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Sample records for junction tj proteins

  1. Tight junction proteins contribute to barrier properties in human pleura.

    Science.gov (United States)

    Markov, Alexander G; Voronkova, Maria A; Volgin, George N; Yablonsky, Piotr K; Fromm, Michael; Amasheh, Salah

    2011-03-15

    The permeability of pleural mesothelium helps to control the volume and composition of the liquid lubricating pleural surfaces. Information on pleural barrier function in health and disease, however, is scarce. Tissue specimens of human pleura were mounted in Ussing chambers for measurement of transmesothelial resistance. Expression of tight junction (TJ) proteins was studied by Western blots and immune fluorescence confocal microscopy. Both visceral and parietal pleura showed barrier properties represented by transmesothelial resistance. Occludin, claudin-1, -3, -5, and -7, were detected in visceral pleura. In parietal pleura, the same TJ proteins were detected, except claudin-7. In tissues from patients with pleural inflammation these tightening claudins were decreased and in visceral pleura claudin-2, a paracellular channel former, became apparent. We report that barrier function in human pleura coincides with expression of claudins known to be key determinants of epithelial barrier properties. In inflamed tissue, claudin expression indicates a reduced barrier function. Copyright © 2010 Elsevier B.V. All rights reserved.

  2. Gap junctions and connexin-interacting proteins

    NARCIS (Netherlands)

    Giepmans, Ben N G

    2004-01-01

    Gap junctions form channels between adjacent cells. The core proteins of these channels are the connexins. Regulation of gap junction communication (GJC) can be modulated by connexin-associating proteins, such as regulatory protein phosphatases and protein kinases, of which c-Src is the

  3. Autophagy and tight junction proteins in the intestine and intestinal diseases

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    Chien-An A. Hu

    2015-09-01

    Full Text Available The intestinal epithelium (IE forms an indispensible barrier and interface between the intestinal interstitium and the luminal environment. The IE regulates water, ion and nutrient transport while providing a barrier against toxins, pathogens (bacteria, fungi and virus and antigens. The apical intercellular tight junctions (TJ are responsible for the paracellular barrier function and regulate trans-epithelial flux of ions and solutes between adjacent cells. Increased intestinal permeability caused by defects in the IE TJ barrier is considered an important pathogenic factor for the development of intestinal inflammation, diarrhea and malnutrition in humans and animals. In fact, defects in the IE TJ barrier allow increased antigenic penetration, resulting in an amplified inflammatory response in inflammatory bowel disease (IBD, necrotizing enterocolitis and ischemia-reperfusion injury. Conversely, the beneficial enhancement of the intestinal TJ barrier has been shown to resolve intestinal inflammation and apoptosis in both animal models of IBD and human IBD. Autophagy (self-eating mechanism is an intracellular lysosome-dependent degradation and recycling pathway essential for cell survival and homeostasis. Dysregulated autophagy has been shown to be directly associated with many pathological processes, including IBD. Importantly, the crosstalk between IE TJ and autophagy has been revealed recently. We showed that autophagy enhanced IE TJ barrier function by increasing transepithelial resistance and reducing the paracellular permeability of small solutes and ions, which is, in part, by targeting claudin-2, a cation-selective, pore-forming, transmembrane TJ protein, for lysosome (autophagy-mediated degradation. Interestingly, previous studies have shown that the inflamed intestinal mucosa in patients with active IBD has increased claudin-2 expression. In addition, inflammatory cytokines (for example, tumor necrosis factor-α, interleukin-6

  4. Actin-interacting protein 1 controls assembly and permeability of intestinal epithelial apical junctions.

    Science.gov (United States)

    Lechuga, Susana; Baranwal, Somesh; Ivanov, Andrei I

    2015-05-01

    Adherens junctions (AJs) and tight junctions (TJs) are crucial regulators of the integrity and restitution of the intestinal epithelial barrier. The structure and function of epithelial junctions depend on their association with the cortical actin cytoskeleton that, in polarized epithelial cells, is represented by a prominent perijunctional actomyosin belt. The assembly and stability of the perijunctional cytoskeleton is controlled by constant turnover (disassembly and reassembly) of actin filaments. Actin-interacting protein (Aip) 1 is an emerging regulator of the actin cytoskeleton, playing a critical role in filament disassembly. In this study, we examined the roles of Aip1 in regulating the structure and remodeling of AJs and TJs in human intestinal epithelium. Aip1 was enriched at apical junctions in polarized human intestinal epithelial cells and normal mouse colonic mucosa. Knockdown of Aip1 by RNA interference increased the paracellular permeability of epithelial cell monolayers, decreased recruitment of AJ/TJ proteins to steady-state intercellular contacts, and attenuated junctional reassembly in a calcium-switch model. The observed defects of AJ/TJ structure and functions were accompanied by abnormal organization and dynamics of the perijunctional F-actin cytoskeleton. Moreover, loss of Aip1 impaired the apico-basal polarity of intestinal epithelial cell monolayers and inhibited formation of polarized epithelial cysts in 3-D Matrigel. Our findings demonstrate a previously unanticipated role of Aip1 in regulating the structure and remodeling of intestinal epithelial junctions and early steps of epithelial morphogenesis. Copyright © 2015 the American Physiological Society.

  5. Tight junction protein ZO-2 expression and relative function of ZO-1 and ZO-2 during mouse blastocyst formation

    International Nuclear Information System (INIS)

    Sheth, Bhavwanti; Nowak, Rachael L.; Anderson, Rebecca; Kwong, Wing Yee; Papenbrock, Thomas; Fleming, Tom P.

    2008-01-01

    Apicolateral tight junctions (TJs) between epithelial cells are multiprotein complexes regulating membrane polarity and paracellular transport and also contribute to signalling pathways affecting cell proliferation and gene expression. ZO-2 and other ZO family members form a sub-membranous scaffold for binding TJ constituents. We investigated ZO-2 contribution to TJ biogenesis and function during trophectoderm epithelium differentiation in mouse preimplantation embryos. Our data indicate that ZO-2 is expressed from maternal and embryonic genomes with maternal ZO-2 protein associated with nuclei in zygotes and particularly early cleavage stages. Embryonic ZO-2 assembled at outer blastomere apicolateral junctional sites from the late 16-cell stage. Junctional ZO-2 first co-localised with E-cadherin in a transient complex comprising adherens junction and TJ constituents before segregating to TJs after their separation from the blastocyst stage (32-cell onwards). ZO-2 siRNA microinjection into zygotes or 2-cell embryos resulted in specific knockdown of ZO-2 mRNA and protein within blastocysts. Embryos lacking ZO-2 protein at trophectoderm TJs exhibited delayed blastocoel cavity formation but underwent normal cell proliferation and outgrowth morphogenesis. Quantitative analysis of trophectoderm TJs in ZO-2-deficient embryos revealed increased assembly of ZO-1 but not occludin, indicating ZO protein redundancy as a compensatory mechanism contributing to the mild phenotype observed. In contrast, ZO-1 knockdown, or combined ZO-1 and ZO-2 knockdown, generated a more severe inhibition of blastocoel formation indicating distinct roles for ZO proteins in blastocyst morphogenesis

  6. Garlic (Allium sativum) feeding impairs Sertoli cell junctional proteins in male Wistar rat testis: microscopy study.

    Science.gov (United States)

    Hammami, I; Nahdi, A; Atig, F; El May, A; El May, M V

    2016-12-01

    Sertoli cell junctions, such as adhesion junction (AJ), gap junction (GJ) and tight junction (TJ), are important for maintaining spermatogenesis. In previous studies, we showed the inhibitory effect of crude garlic (Allium sativum, As) on spermatogenesis and steroidogenesis. The aim of this work was to complete our investigation on the impact of this plant, especially on Sertoli cell junctional proteins (SCJPs). During 1 month, 24 male rats were divided into groups: group control (0% of As) and treated groups fed 5%, 10% and 15% of As. Light and electron microscopy observations were performed to localise junctional proteins: connexin-43, Zona Occluding-1 and N-cadherin (immunohistochemistry) and to describe junctions. We showed that the specific cells involved in the localisation of the SCJP were similar in both control and treated groups, but with different immunoreactivity intensity between them. The electron microscopy observation focused on TJs between Sertoli cells, constituting the blood-testis barrier, showed ultrastructural changes such as fragmentation of TJs between adjacent Sertoli cell membranes and dilatation of rough endoplasmic reticulum saccules giving an aspect of scale to these junctions. We concluded that crude garlic consumption during 1 month induces perturbations on Sertoli cell junctions. These alterations can explain apoptosis in testicular germ cells previously showed. © 2016 Blackwell Verlag GmbH.

  7. Hydrothermal fluid flow within a tectonically active rift-ridge transform junction: Tjörnes Fracture Zone, Iceland

    Science.gov (United States)

    Lupi, M.; Geiger, S.; Graham, C. M.

    2010-05-01

    We investigate the regional fluid flow dynamics in a highly faulted transform area, the Tjörnes Fracture Zone in northern Iceland which is characterized by steep geothermal gradients, hydrothermal activity, and strong seismicity. We simulate fluid flow within the Tjörnes Fracture Zone using a high-resolution model that was based on the available geological and geophysical data and has the aim to represent the complex geological structures and the thermodynamical processes that drive the regional fluid flow in a physically realistic way. Our results show that convective heat flow and mixing of cold and saline seawater with deep hydrothermal fluids controls the large-scale fluid flow. The distribution of faults has a strong influence on the local hydrodynamics by focusing flow around clusters of faults. This explains the nature of isolated upflow zones of hot hydrothermal fluids which are observed in the Tjörnes Fracture Zone. An important emergent characteristic of the regional fluid flow in the Tjörnes Fracture Zone are two separate flow systems: one in the sedimentary basins, comprising more vigorous convection, and one in the crystalline basement, which is dominated by conduction. These two flow systems yield fundamental insight into the connection between regional hydrothermal fluid flow and seismicity because they form the basis of a toggle switch mechanism that is thought to have caused the hydrogeochemical anomalies recorded at Húsavik before and after the 5.8 M earthquake in September 2002.

  8. Lymphocytes accelerate epithelial tight junction assembly: role of AMP-activated protein kinase (AMPK.

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    Xiao Xiao Tang

    2010-08-01

    Full Text Available The tight junctions (TJs, characteristically located at the apicolateral borders of adjacent epithelial cells, are required for the proper formation of epithelial cell polarity as well as for sustaining the mucosal barrier to the external environment. The observation that lymphocytes are recruited by epithelial cells to the sites of infection [1] suggests that they may play a role in the modulation of epithelial barrier function and thus contribute to host defense. To test the ability of lymphocytes to modulate tight junction assembly in epithelial cells, we set up a lymphocyte-epithelial cell co-culture system, in which Madin-Darby canine kidney (MDCK cells, a well-established model cell line for studying epithelial TJ assembly [2], were co-cultured with mouse lymphocytes to mimic an infection state. In a typical calcium switch experiment, the TJ assembly in co-culture was found to be accelerated compared to that in MDCK cells alone. This accelaration was found to be mediated by AMP-activated protein kinase (AMPK. AMPK activation was independent of changes in cellular ATP levels but it was found to be activated by the pro-inflammatory cytokine TNF-alpha. Forced suppression of AMPK, either with a chemical inhibitor or by knockdown, abrogated the accelerating effect of lymphocytes on TJ formation. Similar results were also observed in a co-culture with lymphocytes and Calu-3 human airway epithelial cells, suggesting that the activation of AMPK may be a general mechanism underlying lymphocyte-accelerated TJ assembly in different epithelia. These results suggest that signals from lymphocytes, such as cytokines, facilitate TJ assembly in epithelial cells via the activation of AMPK.

  9. A high-grain diet alters the omasal epithelial structure and expression of tight junction proteins in a goat model.

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    Liu, Jun-Hua; Xu, Ting-Ting; Zhu, Wei-Yun; Mao, Sheng-Yong

    2014-07-01

    The omasal epithelial barrier plays important roles in maintaining nutrient absorption and immune homeostasis in ruminants. However, little information is currently available about the changes in omasal epithelial barrier function at the structural and molecular levels during feeding of a high-grain (HG) diet. Ten male goats were randomly assigned to two groups, fed either a hay diet (0% grain; n = 5) or HG diet (65% grain; n = 5). Changes in omasal epithelial structure and expression of tight junction (TJ) proteins were determined via electron microscopy and Western blot analysis. After 7 weeks on each diet, omasal contents in the HG group showed significantly lower pH (P diet showed profound alterations in omasal epithelial structure and TJ proteins, corresponding to depression of thickness of total epithelia, stratum granulosum, and the sum of the stratum spinosum and stratum basale, marked epithelial cellular damage, erosion of intercellular junctions and down-regulation in expression of the TJ proteins, claudin-4 and occludin. The study demonstrates that feeding a HG diet is associated with omasal epithelial cellular damage and changes in expression of TJ proteins. These research findings provide an insight into the possible significance of diet on the omasal epithelial barrier in ruminants. Copyright © 2014 Elsevier Ltd. All rights reserved.

  10. Possible role of HIWI2 in modulating tight junction proteins in retinal pigment epithelial cells through Akt signaling pathway.

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    Sivagurunathan, Suganya; Palanisamy, Karthikka; Arunachalam, Jayamuruga Pandian; Chidambaram, Subbulakshmi

    2017-03-01

    PIWI subfamily of proteins is shown to be primarily expressed in germline cells. They maintain the genomic integrity by silencing the transposable elements. Although the role of PIWI proteins in germ cells has been documented, their presence and function in somatic cells remains unclear. Intriguingly, we detected all four members of PIWI-like proteins in human ocular tissues and somatic cell lines. When HIWI2 was knocked down in retinal pigment epithelial cells, the typical honeycomb morphology was affected. Further analysis showed that the expression of tight junction (TJ) proteins, CLDN1, and TJP1 were altered in HIWI2 knockdown. Moreover, confocal imaging revealed disrupted TJP1 assembly at the TJ. Previous studies report the role of GSK3β in regulating TJ proteins. Accordingly, phospho-kinase proteome profiler array indicated increased phosphorylation of Akt and GSK3α/β in HIWI2 knockdown, suggesting that HIWI2 might affect TJ proteins through Akt-GSK3α/β signaling axis. Moreover, treating the HIWI2 knockdown cells with wortmannin increased the levels of TJP1 and CLDN1. Taken together, our study demonstrates the presence of PIWI-like proteins in somatic cells and the possible role of HIWI2 in preserving the functional integrity of epithelial cells probably by modulating the phosphorylation status of Akt.

  11. Rescue of perfluorooctanesulfonate (PFOS)-mediated Sertoli cell injury by overexpression of gap junction protein connexin 43

    Science.gov (United States)

    Li, Nan; Mruk, Dolores D.; Chen, Haiqi; Wong, Chris K. C.; Lee, Will M.; Cheng, C. Yan

    2016-07-01

    Perfluorooctanesulfonate (PFOS) is an environmental toxicant used in developing countries, including China, as a stain repellent for clothing, carpets and draperies, but it has been banned in the U.S. and Canada since the late 2000s. PFOS perturbed the Sertoli cell tight junction (TJ)-permeability barrier, causing disruption of actin microfilaments in cell cytosol, perturbing the localization of cell junction proteins (e.g., occluden-ZO-1, N-cadherin-ß-catenin). These changes destabilized Sertoli cell blood-testis barrier (BTB) integrity. These findings suggest that human exposure to PFOS might induce BTB dysfunction and infertility. Interestingly, PFOS-induced Sertoli cell injury associated with a down-regulation of the gap junction (GJ) protein connexin43 (Cx43). We next investigated if overexpression of Cx43 in Sertoli cells could rescue the PFOS-induced cell injury. Indeed, overexpression of Cx43 in Sertoli cells with an established TJ-barrier blocked the disruption in PFOS-induced GJ-intercellular communication, resulting in the re-organization of actin microfilaments, which rendered them similar to those in control cells. Furthermore, cell adhesion proteins that utilized F-actin for attachment became properly distributed at the cell-cell interface, resealing the disrupted TJ-barrier. In summary, Cx43 is a good target that might be used to manage PFOS-induced reproductive dysfunction.

  12. The tight junction protein Z O-2 has several functional nuclear export signals

    International Nuclear Information System (INIS)

    Gonzalez-Mariscal, Lorenza; Ponce, Arturo; Alarcon, Lourdes; Jaramillo, Blanca Estela

    2006-01-01

    The tight junction (TJ) protein ZO-2 changes its subcellular distribution according to the state of confluency of the culture. Thus in confluent monolayers, it localizes at the TJ region whereas in sparse cultures it concentrates at the nucleus. The canine sequence of ZO-2 displays four putative nuclear export signals (NES), two at the second PDZ domain (NES-0 and NES-1) and the rest at the GK region (NES-2 and NES-3). The functionality of NES-0 and NES-3 was unknown, hence here we have explored it with a nuclear export assay, injecting into the nucleus of MDCK cells peptides corresponding to the ZO-2 NES sequences chemically coupled to ovalbumin. We show that both NES-0 and NES-3 are functional and sensitive to leptomycin B. We also demonstrate that NES-1, previously characterized as a non functional NES, is rendered capable of nuclear export upon the acquisition of a negative charge at its Ser369 residue. Experiments performed injecting at the nucleus WT and mutated ZO-2-GST fusion proteins revealed the need of both NES-0 and NES-1, and NES-2 and NES-3 for attaining an efficient nuclear exit of the respective amino and middle segments of ZO-2. Moreover, the transfection of MDCK cells with full-length ZO-2 revealed that the mutation of any of the NES present in the molecule was sufficient to induce nuclear accumulation of the protein

  13. Decaffeinated coffee consumption induces expression of tight junction proteins in high fat diet fed rats

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    Mazzone G

    2016-09-01

    Full Text Available Background: Recent evidence indicates that gut microbiota plays a key role in the development of NAFLD through the gut-liver axis. An altered gut permeability induced by alterations of tight junction (TJ proteins allows the passage of bacteria and substances leading to liver inflammation, hepatocyte damage and fibrosis. This study aims to evaluate the influence of decaffeinated coffee on gut permeability in a rat model of fat liver damage induced by a high fat diet (HFD. Methods: Twelve male Wistar rats were assigned to 3 groups. The first group received HFD for 5 months and drank water. The second group received HFD for 5 months and drank water added with 1.2mL decaffeinated coffee/day starting from the 4th month. The third group received standard diet (SD and drank water. Protein and mRNA expression levels of Toll-Like Receptor- 4 (TLR-4, Occludin and Zonula occludens-1 (ZO-1 were assessed in rat intestines. Results: A significant reduction of Occludin and ZO-1 was observed in HFD fed rats (0.97±0.05 vs 0.15±0.08 p˂0.01, and 0.97±0.05 vs 0.57±0.14 p˂0.001 respectively. This reduction was reverted in HFD+COFFEE rats (0.15±0.08 vs 0.83±0.27 p˂0.01 and 0.57±0.14 vs 0.85±0.12 p˂0.01 respectively. The TLR-4 expression up-regulated by HFD was partially reduced by coffee administration. Conclusions: HFD impairs the intestinal TJ barrier integrity. Coffee increases the expression of TJ proteins, reverting the altered gut permeability and reducing TLR-4 expression.

  14. Attenuated expression of the tight junction proteins is involved in clopidogrel-induced gastric injury through p38 MAPK activation

    International Nuclear Information System (INIS)

    Wu, Hai-Lu; Gao, Xin; Jiang, Zong-Dan; Duan, Zhao-Tao; Wang, Shu-Kui; He, Bang-Shun; Zhang, Zhen-Yu; Xie, Hong-Guang

    2013-01-01

    Highlights: ► Clopidogrel suppressed GES-1 cell viability in a concentration- and time-dependent manner. ► Clopidogrel significantly increased dextran permeability, reduced occludin and ZO-1 expression, and induced cell apoptosis. ► Clopidogrel activated p38 MAPK signaling pathway. ► Activation of p38 activity was involved in clopidogrel-induced increase in gastric epithelial cells permeability and disruption of TJ. -- Abstract: Bleeding complications and delayed healing of gastric ulcer associated with use of clopidogrel is a common clinical concern; however, the underlying mechanisms remain to be determined. This study aimed to clarify whether clopidogrel could cause the damage of the human gastric epithelial cells and to further elucidate the mechanisms involved. After human gastric epithelial cell line GES-1 had been treated with clopidogrel (0.5–2.5 mM), the cell proliferation was examined by MTT assay, apoptosis was measured with DAPI staining and flow cytometry analysis, and the barrier function of the tight junctions (TJ) was evaluated by permeability measurement and transmission electron microscopy. Moreover, expression of the TJ proteins occludin and ZO-1 and the phosphorylation of the mitogen-activated protein kinases (MAPK) p38, ERK, and JNK were examined by western blot. In addition, three MAPK inhibitors specific to p38, ERK and JNK were used, respectively, to verify the signaling pathways responsible for regulating the expression of the TJ proteins being tested. Results showed that clopidogrel significantly increased dextran permeability, induced apoptosis, suppressed GES-1 cell viability, and reduced the expression of the TJ proteins (occludin and ZO-1), acting through p38 MAPK phosphorylation. Furthermore, these observed effects were partially abolished by SB-203580 (a p38 MAPK inhibitor), rather than by either U-0126 (an ERK inhibitor) or SP-600125 (a JNK inhibitor), suggesting that clopidogrel-induced disruption in the gastric

  15. Dietary fat and bile juice, but not obesity, are responsible for the increase in small intestinal permeability induced through the suppression of tight junction protein expression in LETO and OLETF rats

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    Suzuki Takuya

    2010-03-01

    Full Text Available Abstract Background An increase in the intestinal permeability is considered to be associated with the inflammatory tone and development in the obesity and diabetes, however, the pathogenesis of the increase in the intestinal permeability is poorly understood. The present study was performed to determine the influence of obesity itself as well as dietary fat on the increase in intestinal permeability. Methods An obese rat strain, Otsuka Long Evans Tokushima Fatty (OLETF, and the lean counter strain, Long Evans Tokushima Otsuka (LETO, were fed standard or high fat diets for 16 weeks. Glucose tolerance, intestinal permeability, intestinal tight junction (TJ proteins expression, plasma bile acids concentration were evaluated. In addition, the effects of rat bile juice and dietary fat, possible mediators of the increase in the intestinal permeability in the obesity, on TJ permeability were explored in human intestinal Caco-2 cells. Results The OLETF rats showed higher glucose intolerance than did the LETO rats, which became more marked with the prolonged feeding of the high fat diet. Intestinal permeability in the OLETF rats evaluated by the urinary excretion of intestinal permeability markers (Cr-EDTA and phenolsulfonphthalein was comparable to that in the LETO rats. Feeding the high fat diet increased intestinal permeability in both the OLETF and LETO rats, and the increases correlated with decreases in TJ proteins (claudin-1, claudin-3, occludin and junctional adhesion molecule-1 expression in the small, but not in the large intestine (cecum or colon. The plasma bile acids concentration was higher in rats fed the high fat diet. Exposure to bile juice and the fat emulsion increased TJ permeability with concomitant reductions in TJ protein expression (claudin-1, claudin-3, and junctional adhesion molecule-1 in the Caco-2 cell monolayers. Conclusion Excessive dietary fat and/or increased levels of luminal bile juice, but not genetic obesity, are

  16. Volatile anesthetics influence blood-brain barrier integrity by modulation of tight junction protein expression in traumatic brain injury.

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    Serge C Thal

    Full Text Available Disruption of the blood-brain barrier (BBB results in cerebral edema formation, which is a major cause for high mortality after traumatic brain injury (TBI. As anesthetic care is mandatory in patients suffering from severe TBI it may be important to elucidate the effect of different anesthetics on cerebral edema formation. Tight junction proteins (TJ such as zonula occludens-1 (ZO-1 and claudin-5 (cl5 play a central role for BBB stability. First, the influence of the volatile anesthetics sevoflurane and isoflurane on in-vitro BBB integrity was investigated by quantification of the electrical resistance (TEER in murine brain endothelial monolayers and neurovascular co-cultures of the BBB. Secondly brain edema and TJ expression of ZO-1 and cl5 were measured in-vivo after exposure towards volatile anesthetics in native mice and after controlled cortical impact (CCI. In in-vitro endothelial monocultures, both anesthetics significantly reduced TEER within 24 hours after exposure. In BBB co-cultures mimicking the neurovascular unit (NVU volatile anesthetics had no impact on TEER. In healthy mice, anesthesia did not influence brain water content and TJ expression, while 24 hours after CCI brain water content increased significantly stronger with isoflurane compared to sevoflurane. In line with the brain edema data, ZO-1 expression was significantly higher in sevoflurane compared to isoflurane exposed CCI animals. Immunohistochemical analyses revealed disruption of ZO-1 at the cerebrovascular level, while cl5 was less affected in the pericontusional area. The study demonstrates that anesthetics influence brain edema formation after experimental TBI. This effect may be attributed to modulation of BBB permeability by differential TJ protein expression. Therefore, selection of anesthetics may influence the barrier function and introduce a strong bias in experimental research on pathophysiology of BBB dysfunction. Future research is required to investigate

  17. Mast Cell Tryptase Reduces Junctional Adhesion Molecule-A (JAM-A) Expression in Intestinal Epithelial Cells: Implications for the Mechanisms of Barrier Dysfunction in Irritable Bowel Syndrome.

    LENUS (Irish Health Repository)

    Wilcz-Villega, Ewa M

    2013-07-01

    The objective of this study was to investigate how mast cell tryptase may influence intestinal permeability and tight junction (TJ) proteins in vitro and explore translation to irritable bowel syndrome (IBS).

  18. The Effects of Glucagon-like Peptide-2 on the Tight Junction and Barrier Function in IPEC-J2 Cells through Phosphatidylinositol 3-kinase–Protein Kinase B–Mammalian Target of Rapamycin Signaling Pathway

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    Changsong Yu

    2016-05-01

    Full Text Available Glucagon-like peptide-2 (GLP-2 is important for intestinal barrier function and regulation of tight junction (TJ proteins, but the intracellular mechanisms of action remain undefined. The purpose of this research was to determine the protective effect of GLP-2 mediated TJ and transepithelial electrical resistance (TER in lipopolysaccharide (LPS stressed IPEC-J2 cells and to test the hypothesis that GLP-2 regulate TJ and TER through the phosphatidylinositol 3-kinase (PI3K-protein kinase B (Akt-mammalian target of rapamycin (mTOR signaling pathway in IPEC-J2 cells. Wortmannin and LY294002 are specific inhibitors of PI3K. The results showed that 100 μg/mL LPS stress decreased TER and TJ proteins occludin, claudin-1 and zonula occludens protein 1 (ZO-1 mRNA, proteins expressions (p<0.01 respectively. GLP-2 (100 nmol/L promote TER and TJ proteins occludin, claudin-1, and zo-1 mRNA, proteins expressions in LPS stressed and normal IPEC-J2 cells (p<0.01 respectively. In normal cells, both wortmannin and LY294002, PI3K inhibitors, prevented the mRNA and protein expressions of Akt and mTOR increase induced by GLP-2 (p<0.01 following with the significant decreasing of occludin, claudin-1, ZO-1 mRNA and proteins expressions and TER (p<0.01. In conclusion, these results indicated that GLP-2 can promote TJ’s expression and TER in LPS stressed and normal IPEC-J2 cells and GLP-2 could regulate TJ and TER through the PI3K/Akt/mTOR pathway.

  19. Gap junction protein connexin-43 interacts directly with microtubules

    NARCIS (Netherlands)

    Giepmans, B N; Verlaan, I; Hengeveld, T; Janssen, H; Calafat, J; Falk, M M; Moolenaar, W H

    2001-01-01

    Gap junctions are specialized cell-cell junctions that mediate intercellular communication. They are composed of connexin proteins, which form transmembrane channels for small molecules [1, 2]. The C-terminal tail of connexin-43 (Cx43), the most widely expressed connexin member, has been implicated

  20. Arsenic downregulates tight junction claudin proteins through p38 and NF-κB in intestinal epithelial cell line, HT-29

    International Nuclear Information System (INIS)

    Jeong, Chang Hee; Seok, Jin Sil; Petriello, Michael C.; Han, Sung Gu

    2017-01-01

    Arsenic is a naturally occurring metalloid that often is found in foods and drinking water. Human exposure to arsenic is associated with the development of gastrointestinal problems such as fluid loss, diarrhea and gastritis. Arsenic is also known to induce toxic responses including oxidative stress in cells of the gastrointestinal track. Tight junctions (TJs) regulate paracellular permeability and play a barrier role by inhibiting the movement of water, solutes and microorganisms in the paracellular space. Since oxidative stress and TJ damage are known to be associated, we examined whether arsenic produces TJ damage such as downregulation of claudins in the human colorectal cell line, HT-29. To confirm the importance of oxidative stress in arsenic-induced TJ damage, effects of the antioxidant compound (e.g., N-acetylcysteine (NAC)) were also determined in cells. HT-29 cells were treated with arsenic trioxide (40 μM, 12 h) to observe the modified expression of TJ proteins. Arsenic decreased expression of TJ proteins (i.e., claudin-1 and claudin-5) and transepithelial electrical resistance (TEER) whereas pretreatment of NAC (5–10 mM, 1 h) attenuated the observed claudins downregulation and TEER. Arsenic treatment produced cellular oxidative stress via superoxide generation and lowering glutathione (GSH) levels, while NAC restored cellular GSH levels and decreased oxidative stress. Arsenic increased phosphorylation of p38 and nuclear translocation of nuclear factor-kappa B (NF-κB) p65, while NAC attenuated these intracellular events. Results demonstrated that arsenic can damage intestinal epithelial cells by proinflammatory process (oxidative stress, p38 and NF-κB) which resulted in the downregulation of claudins and NAC can protect intestinal TJs from arsenic toxicity.

  1. Nance-Horan syndrome protein, NHS, associates with epithelial cell junctions.

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    Sharma, Shiwani; Ang, Sharyn L; Shaw, Marie; Mackey, David A; Gécz, Jozef; McAvoy, John W; Craig, Jamie E

    2006-06-15

    Nance-Horan syndrome, characterized by congenital cataracts, craniofacial, dental abnormalities and mental disturbances, is an X-linked disorder with significant phenotypic heterogeneity. Affected individuals have mutations in the NHS (Nance-Horan syndrome) gene typically resulting in premature truncation of the protein. This report underlines the complexity of the regulation of the NHS gene that transcribes several isoforms. We demonstrate the differential expression of the two NHS isoforms, NHS-A and NHS-1A, and differences in the subcellular localization of the proteins encoded by these isoforms. This may in part explain the pleiotropic features of the syndrome. We show that the endogenous and exogenous NHS-A isoform localizes to the cell membrane of mammalian cells in a cell-type-dependent manner and that it co-localizes with the tight junction (TJ) protein ZO-1 in the apical aspect of cell membrane in epithelial cells. We also show that the NHS-1A isoform is a cytoplasmic protein. In the developing mammalian lens, we found continuous expression of NHS that became restricted to the lens epithelium in pre- and postnatal lens. Consistent with the in vitro findings, the NHS-A isoform associates with the apical cell membrane in the lens epithelium. This study suggests that disturbances in intercellular contacts underlie cataractogenesis in the Nance-Horan syndrome. NHS is the first gene localized at TJs that has been implicated in congenital cataracts.

  2. The effects of electromagnetic pulse on the protein levels of tight junction associated-proteins in the cerebral cortex, hippocampus, heart, lung, and testis of rats.

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    Qiu, LianBo; Chen, Chen; Ding, GuiRong; Zhou, Yan; Zhang, MengYao

    2011-08-01

    To investigate changes in the expression of tight junction (TJ) proteins in the cerebral cortex, hippocampus, heart, lung, and testes of rats after exposure to electromagnetic pulse (EMP). Eighteen adult male Sprague-Dawley rats were divided into sham and exposure groups. The exposure groups received EMP at 200 kV/m for 200 pulses with a repetition rate of 1 Hz. The expression of TJ proteins (ZO-1, occludin, actin) in the several organs was examined by western blotting. ZO-1 levels in the cerebral cortex decreased 1 h and 3 h after EMP exposure compared with sham group (P<0.05). No significant difference was observed for occludin and actin. ZO-1 levels in the hippocampus increased 1 h and 3 h post-exposure (P<0.05), and occludin decreased after 3 h (P<0.05); however, actin was unaffected. ZO-1 levels in the heart increased 3 h post-exposure (P<0.05), occludin decreased 3 h post-exposure (P<0.05), and actin increased 1 h and 3 h post-exposure (P<0.05). ZO-1, occludin and actin levels in the lung decreased compared with those in the sham group (P<0.05). ZO-1 and occludin levels in the testes decreased 1 h and 3 h post-exposure (P<0.05), but actin showed no significant change. Exposure to EMP altered the expression levels of TJ proteins, particularly ZO-1, in the organs of adult male rats, which may induce changes in barrier structure and function. Copyright © 2011 The Editorial Board of Biomedical and Environmental Sciences. Published by Elsevier B.V. All rights reserved.

  3. Sodium caprate transiently opens claudin-5-containing barriers at tight junctions of epithelial and endothelial cells

    DEFF Research Database (Denmark)

    Del Vecchio, Giovanna; Tscheik, Christian; Tenz, Kareen

    2012-01-01

    Claudin-5 is a tight junction (TJ) protein which limits the diffusion of small hydrophilic molecules. Thus, it represents a potential pharmacological target to improve drug delivery to the tissues protected by claudin-5-dependent barriers. Sodium caprate is known as an absorption enhancer which...... opens the paracellular space acting on TJ proteins and actin cytoskeleton. Its action on claudin-5 is not understood so far. Epithelial and endothelial systems were used to evaluate the effect of caprate on claudin-5 in TJ-free cells and on claudin-5 fully integrated in TJ. To this aim, confocal...... of endothelial and epithelial cells. In conclusion, the study further elucidates the cellular effects of caprate at the tight junctions....

  4. Claudin Loss-of-Function Disrupts Tight Junctions and Impairs Amelogenesis.

    Science.gov (United States)

    Bardet, Claire; Ribes, Sandy; Wu, Yong; Diallo, Mamadou Tidiane; Salmon, Benjamin; Breiderhoff, Tilman; Houillier, Pascal; Müller, Dominik; Chaussain, Catherine

    2017-01-01

    Claudins are a family of proteins that forms paracellular barriers and pores determining tight junctions (TJ) permeability. Claudin-16 and -19 are pore forming TJ proteins allowing calcium and magnesium reabsorption in the thick ascending limb of Henle's loop (TAL). Loss-of-function mutations in the encoding genes, initially identified to cause Familial Hypomagnesemia with Hypercalciuria and Nephrocalcinosis (FHHNC), were recently shown to be also involved in Amelogenesis Imperfecta (AI). In addition, both claudins were expressed in the murine tooth germ and Claudin-16 knockout (KO) mice displayed abnormal enamel formation. Claudin-3, an ubiquitous claudin expressed in epithelia including kidney, acts as a barrier-forming tight junction protein. We determined that, similarly to claudin-16 and claudin-19, claudin-3 was expressed in the tooth germ, more precisely in the TJ located at the apical end of secretory ameloblasts. The observation of Claudin-3 KO teeth revealed enamel defects associated to impaired TJ structure at the secretory ends of ameloblasts and accumulation of matrix proteins in the forming enamel. Thus, claudin-3 protein loss-of-function disturbs amelogenesis similarly to claudin-16 loss-of-function, highlighting the importance of claudin proteins for the TJ structure. These findings unravel that loss-of-function of either pore or barrier-forming TJ proteins leads to enamel defects. Hence, the major structural function of claudin proteins appears essential for amelogenesis.

  5. Claudin Loss-of-Function Disrupts Tight Junctions and Impairs Amelogenesis

    Directory of Open Access Journals (Sweden)

    Claire Bardet

    2017-05-01

    Full Text Available Claudins are a family of proteins that forms paracellular barriers and pores determining tight junctions (TJ permeability. Claudin-16 and -19 are pore forming TJ proteins allowing calcium and magnesium reabsorption in the thick ascending limb of Henle's loop (TAL. Loss-of-function mutations in the encoding genes, initially identified to cause Familial Hypomagnesemia with Hypercalciuria and Nephrocalcinosis (FHHNC, were recently shown to be also involved in Amelogenesis Imperfecta (AI. In addition, both claudins were expressed in the murine tooth germ and Claudin-16 knockout (KO mice displayed abnormal enamel formation. Claudin-3, an ubiquitous claudin expressed in epithelia including kidney, acts as a barrier-forming tight junction protein. We determined that, similarly to claudin-16 and claudin-19, claudin-3 was expressed in the tooth germ, more precisely in the TJ located at the apical end of secretory ameloblasts. The observation of Claudin-3 KO teeth revealed enamel defects associated to impaired TJ structure at the secretory ends of ameloblasts and accumulation of matrix proteins in the forming enamel. Thus, claudin-3 protein loss-of-function disturbs amelogenesis similarly to claudin-16 loss-of-function, highlighting the importance of claudin proteins for the TJ structure. These findings unravel that loss-of-function of either pore or barrier-forming TJ proteins leads to enamel defects. Hence, the major structural function of claudin proteins appears essential for amelogenesis.

  6. STIM proteins and the endoplasmic reticulum-plasma membrane junctions.

    Science.gov (United States)

    Carrasco, Silvia; Meyer, Tobias

    2011-01-01

    Eukaryotic organelles can interact with each other through stable junctions where the two membranes are kept in close apposition. The junction that connects the endoplasmic reticulum to the plasma membrane (ER-PM junction) is unique in providing a direct communication link between the ER and the PM. In a recently discovered signaling process, STIM (stromal-interacting molecule) proteins sense a drop in ER Ca(2+) levels and directly activate Orai PM Ca(2+) channels across the junction space. In an inverse process, a voltage-gated PM Ca(2+) channel can directly open ER ryanodine-receptor Ca(2+) channels in striated-muscle cells. Although ER-PM junctions were first described 50 years ago, their broad importance in Ca(2+) signaling, as well as in the regulation of cholesterol and phosphatidylinositol lipid transfer, has only recently been realized. Here, we discuss research from different fields to provide a broad perspective on the structures and unique roles of ER-PM junctions in controlling signaling and metabolic processes.

  7. Tunneling explains efficient electron transport via protein junctions.

    Science.gov (United States)

    Fereiro, Jerry A; Yu, Xi; Pecht, Israel; Sheves, Mordechai; Cuevas, Juan Carlos; Cahen, David

    2018-05-15

    Metalloproteins, proteins containing a transition metal ion cofactor, are electron transfer agents that perform key functions in cells. Inspired by this fact, electron transport across these proteins has been widely studied in solid-state settings, triggering the interest in examining potential use of proteins as building blocks in bioelectronic devices. Here, we report results of low-temperature (10 K) electron transport measurements via monolayer junctions based on the blue copper protein azurin (Az), which strongly suggest quantum tunneling of electrons as the dominant charge transport mechanism. Specifically, we show that, weakening the protein-electrode coupling by introducing a spacer, one can switch the electron transport from off-resonant to resonant tunneling. This is a consequence of reducing the electrode's perturbation of the Cu(II)-localized electronic state, a pattern that has not been observed before in protein-based junctions. Moreover, we identify vibronic features of the Cu(II) coordination sphere in transport characteristics that show directly the active role of the metal ion in resonance tunneling. Our results illustrate how quantum mechanical effects may dominate electron transport via protein-based junctions.

  8. Behavior of tight-junction, adherens-junction and cell polarity proteins during HNF-4α-induced epithelial polarization

    International Nuclear Information System (INIS)

    Satohisa, Seiro; Chiba, Hideki; Osanai, Makoto; Ohno, Shigeo; Kojima, Takashi; Saito, Tsuyoshi; Sawada, Norimasa

    2005-01-01

    We previously reported that expression of tight-junction molecules occludin, claudin-6 and claudin-7, as well as establishment of epithelial polarity, was triggered in mouse F9 cells expressing hepatocyte nuclear factor (HNF)-4α [H. Chiba, T. Gotoh, T. Kojima, S. Satohisa, K. Kikuchi, M. Osanai, N. Sawada. Hepatocyte nuclear factor (HNF)-4α triggers formation of functional tight junctions and establishment of polarized epithelial morphology in F9 embryonal carcinoma cells, Exp. Cell Res. 286 (2003) 288-297]. Using these cells, we examined in the present study behavior of tight-junction, adherens-junction and cell polarity proteins and elucidated the molecular mechanism behind HNF-4α-initiated junction formation and epithelial polarization. We herein show that not only ZO-1 and ZO-2, but also ZO-3, junctional adhesion molecule (JAM)-B, JAM-C and cell polarity proteins PAR-3, PAR-6 and atypical protein kinase C (aPKC) accumulate at primordial adherens junctions in undifferentiated F9 cells. In contrast, CRB3, Pals1 and PATJ appeared to exhibit distinct subcellular localization in immature cells. Induced expression of HNF-4α led to translocation of these tight-junction and cell polarity proteins to beltlike tight junctions, where occludin, claudin-6 and claudin-7 were assembled, in differentiated cells. Interestingly, PAR-6, aPKC, CRB3 and Pals1, but not PAR-3 or PATJ, were also concentrated on the apical membranes in differentiated cells. These findings indicate that HNF-4α provokes not only expression of tight-junction adhesion molecules, but also modulation of subcellular distribution of junction and cell polarity proteins, resulting in junction formation and epithelial polarization

  9. Scutellaria barbata attenuates diabetic retinopathy by preventing retinal inflammation and the decreased expression of tight junction protein

    Directory of Open Access Journals (Sweden)

    Xi-Yu Mei

    2017-06-01

    Full Text Available AIM: To observe the attenuation of ethanol extract of Herba Scutellaria barbata (SE against diabetic retinopathy (DR and its engaged mechanism. METHODS: C57BL/6J mice were intraperitoneally injected with streptozotocin (STZ, 55 mg/kg for 5 consecutive days to induce diabetes. The diabetic mice were orally given with SE (100, 200 mg/kg for 1mo at 1mo after STZ injection. Blood-retinal barrier (BRB breakdown was detected by using Evans blue permeation assay. Real-time polymerase chain reaction (RT-PCR, Western blot and immunofluorescence staining were used to detect mRNA and protein expression. Enzyme-linked immunosorbent assay (ELISA was used to detect serum contents of tumor necrosis factor-α (TNF-α and interleukin (IL-1β. RESULTS: SE (100, 200 mg/kg reversed the breakdown of BRB in STZ-induced diabetic mice. The decreased expression of retinal claudin-1 and claudin-19, which are both tight junction (TJ proteins, was reversed by SE. SE decreased the increased serum contents and retinal mRNA expression of TNF-α and IL-1β. SE also decreased the increased retinal expression of intercellular cell adhesion molecule-1 (ICAM-1. SE reduced the increased phosphorylation of nuclear factor kappa B (NFκB p65 and its subsequent nuclear translocation in retinas from STZ-induced diabetic mice. Results of Western blot and retinal immunofluorescence staining of ionized calcium-binding adapter molecule 1 (Iba1 demonstrated that SE abrogated the activation of microglia cells in STZ-induced diabetic mice. CONCLUSION: SE attenuates the development of DR by inhibiting retinal inflammation and restoring the decreased expression of TJ proteins including claudin-1 and claudin-19.

  10. Expressions of tight junction proteins Occludin and Claudin-1 are under the circadian control in the mouse large intestine: implications in intestinal permeability and susceptibility to colitis.

    Directory of Open Access Journals (Sweden)

    Oh-oka Kyoko

    Full Text Available BACKGROUND & AIMS: The circadian clock drives daily rhythms in behavior and physiology. A recent study suggests that intestinal permeability is also under control of the circadian clock. However, the precise mechanisms remain largely unknown. Because intestinal permeability depends on tight junction (TJ that regulates the epithelial paracellular pathway, this study investigated whether the circadian clock regulates the expression levels of TJ proteins in the intestine. METHODS: The expression levels of TJ proteins in the large intestinal epithelium and colonic permeability were analyzed every 4, 6, or 12 hours between wild-type mice and mice with a mutation of a key clock gene Period2 (Per2; mPer2(m/m. In addition, the susceptibility to dextran sodium sulfate (DSS-induced colitis was compared between wild-type mice and mPer2(m/m mice. RESULTS: The mRNA and protein expression levels of Occludin and Claudin-1 exhibited daily variations in the colonic epithelium in wild-type mice, whereas they were constitutively high in mPer2(m/m mice. Colonic permeability in wild-type mice exhibited daily variations, which was inversely associated with the expression levels of Occludin and Claudin-1 proteins, whereas it was constitutively low in mPer2(m/m mice. mPer2(m/m mice were more resistant to the colonic injury induced by DSS than wild-type mice. CONCLUSIONS: Occludin and Claudin-1 expressions in the large intestine are under the circadian control, which is associated with temporal regulation of colonic permeability and also susceptibility to colitis.

  11. TJ-II project

    International Nuclear Information System (INIS)

    Alejaldre, C.; Gozalo, J.J.A.; Perez, J.B.; Magaria, F.C.; Diaz, J.R.C.; Perez, J.G.; Lopez-Fraguas, A.; Garcia, L.; Krivenski, V.I.; Martin, R.; Navarro, A.P.; Perea, A.; Rodriguez-Yunta, A.; Ayza, M.S.; Varias, A.

    1990-01-01

    The TJ-II device is a medium-size helical-axis stellarator to be built in Madrid. Its main characteristics are potential for high-beta operation; flexibility, i.e., its rotational transform can be varied over a wide range and its shear to some extent; and bean-shaped plasma cross section. The latest understanding of TJ-II physics in the fields of electron cyclotron resonance heating, transport, and magneto-hydrodynamics, and the engineering solutions introduced in its final design are discussed

  12. Claudin-11 and occludin are major contributors to Sertoli cell tight junction function, in vitro

    Directory of Open Access Journals (Sweden)

    Mark J McCabe

    2016-01-01

    Full Text Available The Sertoli cell tight junction (TJ is the key component of the blood-testis barrier, where it sequesters developing germ cells undergoing spermatogenesis within the seminiferous tubules. Hormonally regulated claudin-11 is a critical transmembrane protein involved in barrier function and its murine knockout results in infertility. We aimed to assess quantitatively the significance of the contribution of claudin-11 to TJ function, in vitro, using siRNA-mediated gene silencing. We also conducted an analysis of the contribution of occludin, another intrinsic transmembrane protein of the TJ. Silencing of claudin-11 and/or occludin was conducted using siRNA in an immature rat Sertoli cell culture model. Transepithelial electrical resistance was used to assess quantitatively TJ function throughout the culture. Two days after siRNA treatment, cells were fixed for immunocytochemical localization of junction proteins or lyzed for RT-PCR assessment of mRNA expression. Silencing of claudin-11, occludin, or both resulted in significant decreases in TJ function of 55% (P < 0.01, 51% (P < 0.01, and 62% (P < 0.01, respectively. Data were concomitant with significant decreases in mRNA expression and marked reductions in the localization of targeted proteins to the Sertoli cell TJ. We provide quantitative evidence that claudin-11 contributes significantly (P < 0.01 to Sertoli cell TJ function in vitro. Interestingly, occludin, which is hormonally regulated but not implicated in infertility until late adulthood, is also a significant (P < 0.01 contributor to barrier function. Our data are consistent with in vivo studies that clearly demonstrate a role for these proteins in maintaining normal TJ barrier structure and function.

  13. Modulation of Tight Junction Structure and Function by Kinases and Phosphatases Targeting Occludin

    Directory of Open Access Journals (Sweden)

    Max Johannes Dörfel

    2012-01-01

    Full Text Available Tight junctions (TJs typically represent the most apical contacts in epithelial and endothelial cell layers where they play an essential role in the separation of extracellular or luminal spaces from underlying tissues in the body. Depending on the protein composition, TJs define the barrier characteristics and in addition maintain cell polarity. Two major families of integral membrane proteins form the typical TJ strand network, the tight junction-associated MARVEL protein (TAMP family members occludin, tricellulin, and MarvelD3 as well as a specific set of claudins. Occludin was the first identified member of these tetraspanins and is now widely accepted as a regulator of TJ assembly and function. Therefore, occludin itself has to be tightly regulated. Phosphorylation of occludin appears to be of central importance in this context. Here we want to summarize current knowledge on the kinases and phosphatases directly modifying occludin, and their role in the regulation of TJ structure, function, and dynamics.

  14. Role of the Adherens Junction Protein Fascin in the Regulation of Tight Junction Permeability in the Mouse Mammary Gland

    National Research Council Canada - National Science Library

    Beeman, Neal

    2001-01-01

    .... Transduced cells are morphologically normal and produce milk. This gene delivery system was used to express an N-terminally truncated mutant of the tight junction protein occluding in the mammary gland and in cultured cells...

  15. Moderate hypoxia followed by reoxygenation results in blood-brain barrier breakdown via oxidative stress-dependent tight-junction protein disruption.

    Directory of Open Access Journals (Sweden)

    Christoph M Zehendner

    Full Text Available Re-canalization of cerebral vessels in ischemic stroke is pivotal to rescue dysfunctional brain areas that are exposed to moderate hypoxia within the penumbra from irreversible cell death. Goal of the present study was to evaluate the effect of moderate hypoxia followed by reoxygenation (MHR on the evolution of reactive oxygen species (ROS and blood-brain barrier (BBB integrity in brain endothelial cells (BEC. BBB integrity was assessed in BEC in vitro and in microvessels of the guinea pig whole brain in situ preparation. Probes were exposed to MHR (2 hours 67-70 mmHg O2, 3 hours reoxygenation, BEC or towards occlusion of the arteria cerebri media (MCAO with or without subsequent reperfusion in the whole brain preparation. In vitro BBB integrity was evaluated using trans-endothelial electrical resistance (TEER and transwell permeability assays. ROS in BEC were evaluated using 2',7'-dichlorodihydrofluorescein diacetate (DCF, MitoSox and immunostaining for nitrotyrosine. Tight-junction protein (TJ integrity in BEC, stainings for nitrotyrosine and FITC-albumin extravasation in the guinea pig brain preparation were assessed by confocal microscopy. Diphenyleneiodonium (DPI was used to investigate NADPH oxidase dependent ROS evolution and its effect on BBB parameters in BEC. MHR impaired TJ proteins zonula occludens 1 (ZO-1 and claudin 5 (Cl5, decreased TEER, and significantly increased cytosolic ROS in BEC. These events were blocked by the NADPH oxidase inhibitor DPI. MCAO with or without subsequent reoxygenation resulted in extravasation of FITC-albumin and ROS generation in the penumbra region of the guinea pig brain preparation and confirmed BBB damage. BEC integrity may be impaired through ROS in MHR on the level of TJ and the BBB is also functionally impaired in moderate hypoxic conditions followed by reperfusion in a complex guinea pig brain preparation. These findings suggest that the BBB is susceptible towards MHR and that ROS play a key role

  16. Alterations of Intercellular Junctions in Peritoneal Mesothelial Cells from Patients Undergoing Dialysis: Effect of Retinoic Acid

    Science.gov (United States)

    Retana, Carmen; Sanchez, Elsa; Perez-Lopez, Alejandro; Cruz, Armando; Lagunas, Jesus; Cruz, Carmen; Vital, Socorro; Reyes, Jose L.

    2015-01-01

    ♦ Background: Dialysis patients are classified according to their peritoneal permeability as low transporter (LT, low solute permeability) or high transporter (HT, high solute permeability). Tight junction (TJ) proteins are critical to maintain ions, molecules and water paracellular transport through peritoneum. Exposure to peritoneal dialysis solutions causes damage to TJ in human peritoneal mesothelial cells (HPMCs). We analyzed the quantity, distribution and function of TJ proteins: claudin-1, -2 and -8, ZO-1 and occludin, in HPMC cultures from LT and HT patients. Since all-trans retinoic acid (ATRA) might modify the expression of TJ proteins, we studied its effect on HPMCs. ♦ Methods: Control HPMCs were isolated from human omentum, while HT or LT cells were obtained from dialysis effluents. Cells were cultured in presence of ATRA 0, 50 or 100 nM. Transepithelial electrical resistance (TER) measurement, immunostaining and Western blot analyses were performed. ♦ Results: HT exhibited lower TER than control and LT monolayers. Immunofluorescence for TJ was weak and discontinuous along the cell contour, in LT and HT. Furthermore, claudin-1, occludin and ZO-1 expressions were decreased. In all groups, claudin-2 was localized at nuclei. We observed that ATRA improved TJ distribution and increased TJ expression in HT. This retinoid did not modify claudin-2 and -8 expressions. All-trans retinoic acid decreased TER in HT, but had no effect in LT. ♦ Conclusions: Tight junctions were altered in HPMCs from dialyzed patients. The HT monolayer has lower TER than LT, which might be associated with the peritoneal permeability in these patients. ATRA might be a therapeutic alternative to maintain mesothelial integrity, since it improved TJ localization and expression. PMID:24584604

  17. Protective Effect of 1,25-Dihydroxy Vitamin D3 on Pepsin-Trypsin-Resistant Gliadin-Induced Tight Junction Injuries.

    Science.gov (United States)

    Dong, Shouquan; Singh, Tikka Prabhjot; Wei, Xin; Yao, Huang; Wang, Hongling

    2018-01-01

    Tight junction (TJ) injuries induced by pepsin-trypsin-resistant gliadin (PT-G) play an important role in the pathogenesis of celiac disease. Previously, 1,25-dihydroxy vitamin D3 (VD3) was reported to be a TJ regulator that attenuates lipopolysaccharide- and alcohol-induced TJ injuries. However, whether VD3 can attenuate PT-G-induced TJ injuries is unknown. The aim of this study was to evaluate the effects of VD3 on PT-G-induced TJ injuries. Caco-2 monolayers were used as in vitro models. After being cultured for 21 days, the monolayers were treated with PT-G plus different concentrations of VD3. Then, the changes in trans-epithelial electrical resistance and FITC-dextran 4000 (FD-4) flux were determined to evaluate the monolayer barrier function. TJ protein levels were measured to assess TJ injury severity, and myeloid differentiation factor 88 (MyD88) expression and zonulin release levels were determined to estimate zonulin release signaling pathway activity. Additionally, a gluten-sensitized mouse model was established as an in vivo model. After the mice were treated with VD3 for 7 days, we measured serum FD-4 concentrations, TJ protein levels, MyD88 expression, and zonulin release levels to confirm the effect of VD3. Both in vitro and in vivo, VD3 significantly attenuated the TJ injury-related increase in intestinal mucosa barrier permeability. Moreover, VD3 treatment up-regulated TJ protein expression levels and significantly decreased MyD88 expression and zonulin release levels. VD3 has protective effects against PT-G-induced TJ injuries both in vitro and in vivo, which may correlate with the disturbance of the MyD88-dependent zonulin release signaling pathway.

  18. Specific protein-protein interactions of calsequestrin with junctional sarcoplasmic reticulum of skeletal muscle

    International Nuclear Information System (INIS)

    Damiani, E.; Margreth, A.

    1990-01-01

    Minor protein components of triads and of sarcoplasmic reticulum (SR) terminal cisternae (TC), i.e. 47 and 37 kDa peptides and 31-30 kDa and 26-25 kDa peptide doublets, were identified from their ability to bind 125 I calsequestrin (CS) in the presence of EGTA. The CS-binding peptides are specifically associated with the junctional membrane of TC, since they could not be detected in junctional transverse tubules and in longitudinal SR fragments. The 31-30 kDa peptide doublet, exclusively, did not bind CS in the presence of Ca 2+ . Thus, different types of protein-protein interactions appear to be involved in selective binding of CS to junctional TC

  19. Gap junction protein connexin43 exacerbates lung vascular permeability.

    Directory of Open Access Journals (Sweden)

    James J O'Donnell

    Full Text Available Increased vascular permeability causes pulmonary edema that impairs arterial oxygenation and thus contributes to morbidity and mortality associated with Acute Respiratory Distress Syndrome and sepsis. Although components of intercellular adhesive and tight junctions are critical for maintaining the endothelial barrier, there has been limited study of the roles of gap junctions and their component proteins (connexins. Since connexins can modulate inflammatory signaling in other systems, we hypothesized that connexins may also regulate pulmonary endothelial permeability. The relationships between connexins and the permeability response to inflammatory stimuli were studied in cultured human pulmonary endothelial cells. Prolonged treatment with thrombin, lipopolysaccharide, or pathological cyclic stretch increased levels of mRNA and protein for the major connexin, connexin43 (Cx43. Thrombin and lipopolysaccharide both increased intercellular communication assayed by transfer of microinjected Lucifer yellow. Although thrombin decreased transendothelial resistance in these cells, the response was attenuated by pretreatment with the connexin inhibitor carbenoxolone. Additionally, the decreases of transendothelial resistance produced by either thrombin or lipopolysaccharide were attenuated by reducing Cx43 expression by siRNA knockdown. Both carbenoxolone and Cx43 knockdown also abrogated thrombin-induced phosphorylation of myosin light chain. Taken together, these data suggest that increased lung vascular permeability induced by inflammatory conditions may be amplified via increased expression of Cx43 and intercellular communication among pulmonary endothelial cells.

  20. Cardiotonic steroid ouabain stimulates expression of blood-testis barrier proteins claudin-1 and -11 and formation of tight junctions in Sertoli cells.

    Science.gov (United States)

    Dietze, Raimund; Shihan, Mazen; Stammler, Angelika; Konrad, Lutz; Scheiner-Bobis, Georgios

    2015-04-15

    The interaction of ouabain with the sodium pump induces signalling cascades resembling those triggered by hormone/receptor interactions. In the rat Sertoli cell line 93RS2, ouabain at low concentrations stimulates the c-Src/c-Raf/Erk1/2 signalling cascade via its interaction with the α4 isoform of the sodium pump expressed in these cells, leading to the activation of the transcription factor CREB. As a result of this signalling sequence, ouabain stimulates expression of claudin-1 and claudin-11, which are also controlled by a CRE promoter. Both of these proteins are known to be essential constituents of tight junctions (TJ) between Sertoli cells, and as a result of the ouabain-induced signalling TJ formation between neighbouring Sertoli cells is significantly enhanced by the steroid. Thus, ouabain-treated cell monolayers display higher transepithelial resistance and reduced free diffusion of FITC-coupled dextran in tracer diffusion assays. Taking into consideration that the formation of TJ is indispensable for the maintenance of the blood-testis barrier (BTB) and therefore for male fertility, the actions of ouabain described here and the fact that this and other related cardiotonic steroids (CTS) are produced endogenously suggest a direct influence of ouabain/sodium pump interactions on the maintenance of the BTB and thereby an effect on male fertility. Since claudin-1 and claudin-11 are also present in other blood-tissue barriers, one can speculate that ouabain and perhaps other CTS influence the dynamics of these barriers as well. Copyright © 2015 Elsevier Ireland Ltd. All rights reserved.

  1. Methamphetamine transiently increases the blood-brain barrier permeability in the hippocampus: role of tight junction proteins and matrix metalloproteinase-9.

    Science.gov (United States)

    Martins, Tânia; Baptista, Sofia; Gonçalves, Joana; Leal, Ermelindo; Milhazes, Nuno; Borges, Fernanda; Ribeiro, Carlos F; Quintela, Oscar; Lendoiro, Elena; López-Rivadulla, Manuel; Ambrósio, António F; Silva, Ana P

    2011-09-09

    Methamphetamine (METH) is a powerful stimulant drug of abuse that has steadily gained popularity worldwide. It is known that METH is highly neurotoxic and causes irreversible damage of brain cells leading to neurological and psychiatric abnormalities. Recent studies suggested that METH-induced neurotoxicity might also result from its ability to compromise blood-brain barrier (BBB) function. Due to the crucial role of BBB in the maintenance of brain homeostasis and protection against toxic molecules and pathogenic organisms, its dysfunction could have severe consequences. In this study, we investigated the effect of an acute high dose of METH (30mg/kg) on BBB permeability after different time points and in different brain regions. For that, young adult mice were sacrificed 1h, 24h or 72h post-METH administration. METH increased BBB permeability, but this effect was detected only at 24h after administration, being therefore a transitory effect. Interestingly, we also found that the hippocampus was the most susceptible brain region to METH, comparing to frontal cortex and striatum. Moreover, in an attempt to identify the key players in METH-induced BBB dysfunction we further investigated potential alterations in tight junction (TJ) proteins and matrix metalloproteinase-9 (MMP-9). METH was able to decrease the protein levels of zonula occludens (ZO)-1, claudin-5 and occludin in the hippocampus 24h post-injection, and increased the activity and immunoreactivity of MMP-9. The pre-treatment with BB-94 (30mg/kg), a matrix metalloproteinase inhibitor, prevented the METH-induced increase in MMP-9 immunoreactivity in the hippocampus. Overall, the present data demonstrate that METH transiently increases the BBB permeability in the hippocampus, which can be explained by alterations on TJ proteins and MMP-9. Copyright © 2011 Elsevier B.V. All rights reserved.

  2. Interleukin-4 and interleukin-13 compromise the sinonasal epithelial barrier and perturb intercellular junction protein expression.

    Science.gov (United States)

    Wise, Sarah K; Laury, Adrienne M; Katz, Elizabeth H; Den Beste, Kyle A; Parkos, Charles A; Nusrat, Asma

    2014-05-01

    Altered expression of epithelial intercellular junction proteins has been observed in sinonasal biopsies from nasal polyps and epithelial layers cultured from nasal polyp patients. These alterations comprise a "leaky" epithelial barrier phenotype. We hypothesize that T helper 2 (Th2) cytokines interleukin (IL)-4 and IL-13 modulate epithelial junction proteins, thereby contributing to the leaky epithelial barrier. Differentiated primary sinonasal epithelial layers cultured at the air-liquid interface were exposed to IL-4, IL-13, and controls for 24 hours at 37°C. Epithelial resistance measurements were taken every 4 hours during cytokine exposure. Western blot and immunofluorescence staining/confocal microscopy were used to assess changes in a panel of tight and adherens junction proteins. Western blot densitometry was quantified with image analysis. IL-4 and IL-13 exposure resulted in a mean decrease in transepithelial resistance at 24 hours to 51.6% (n = 6) and 68.6% (n = 8) of baseline, respectively. Tight junction protein junctional adhesion molecule-A (JAM-A) expression decreased 42.2% with IL-4 exposure (n = 9) and 37.5% with IL-13 exposure (n = 9). Adherens junction protein E-cadherin expression decreased 35.3% with IL-4 exposure (n = 9) and 32.9% with IL-13 exposure (n = 9). Tight junction protein claudin-2 showed more variability but had a trend toward higher expression with Th2 cytokine exposure. There were no appreciable changes in claudin-1, occludin, or zonula occludens-1 (ZO-1) with IL-4 or IL-13 exposure. Sinonasal epithelial exposure to Th2 cytokines IL-4 and IL-13 results in alterations in intercellular junction proteins, reflecting increased epithelial permeability. Such changes may explain some of the phenotypic manifestations of Th2-mediated sinonasal disease, such as edema, nasal discharge, and environmental reactivity. © 2014 ARS-AAOA, LLC.

  3. Tight junction-associated MARVEL proteins marveld3, tricellulin, and occludin have distinct but overlapping functions.

    Science.gov (United States)

    Raleigh, David R; Marchiando, Amanda M; Zhang, Yong; Shen, Le; Sasaki, Hiroyuki; Wang, Yingmin; Long, Manyuan; Turner, Jerrold R

    2010-04-01

    In vitro studies have demonstrated that occludin and tricellulin are important for tight junction barrier function, but in vivo data suggest that loss of these proteins can be overcome. The presence of a heretofore unknown, yet related, protein could explain these observations. Here, we report marvelD3, a novel tight junction protein that, like occludin and tricellulin, contains a conserved four-transmembrane MARVEL (MAL and related proteins for vesicle trafficking and membrane link) domain. Phylogenetic tree reconstruction; analysis of RNA and protein tissue distribution; immunofluorescent and electron microscopic examination of subcellular localization; characterization of intracellular trafficking, protein interactions, dynamic behavior, and siRNA knockdown effects; and description of remodeling after in vivo immune activation show that marvelD3, occludin, and tricellulin have distinct but overlapping functions at the tight junction. Although marvelD3 is able to partially compensate for occludin or tricellulin loss, it cannot fully restore function. We conclude that marvelD3, occludin, and tricellulin define the tight junction-associated MARVEL protein family. The data further suggest that these proteins are best considered as a group with both redundant and unique contributions to epithelial function and tight junction regulation.

  4. CAMAC Software for TJ-I and TJ-IU

    Energy Technology Data Exchange (ETDEWEB)

    Milligen, B Ph. van

    1993-07-01

    A user-friendly software package for control of CAMAC data acquisition modules for the TJ-I and TJ-IU experiments at the Asociacion CIEMAT para Fusion has been developed. The CAMAC control software operates in synchronisation with the pre-existing VME-based data acquisition system. The control software controls the setup of the CAMAC modules and manages the data flow from the lacking to the storage of data. Data file management is performed largely automatically. Further, user software is provided for viewing and analysing the data. (Author) 9 refs.

  5. CAMAC Software for TJ-I and TJ-IU

    International Nuclear Information System (INIS)

    Milligen, B. Ph. van

    1994-01-01

    A user-friendly software package for control of CAMAC data acquisition modules for the TJ-I and TJ-IU experiments at the Asociacion CIEMAT para Fusion has been developed. The CAMAC control software operates in synchronisation with the pre-existing VME-based data acquisition system. The control software controls the setup of the CAMAC modules and manages the data flow from the lacking to the storage of data. Data file management is performed largely automatically. Further, user software is provided for viewing and analysing the data. (Author) 9 refs

  6. Camac Software for TJ-I and TJ-IU

    International Nuclear Information System (INIS)

    Milligen, B. Ph. van.

    1994-01-01

    A user-friendly software package for control of CAMAC data acquisition modules for the TJ-I and TJ-IU experiments at the Association CIEMAT para Fusion has been developed. The CAMAC control software operates in Synchronization with the pre-existing VME-based data-acquisition system. The control software controls the setup of the CAMAC modules and manages the data flow from the taking to the storage of data. Data file management is performed largely automatically. Further, user software is provided for viewing and analysing the data

  7. [6]-Gingerol Prevents Disassembly of Cell Junctions and Activities of MMPs in Invasive Human Pancreas Cancer Cells through ERK/NF-κB/Snail Signal Transduction Pathway

    Directory of Open Access Journals (Sweden)

    Sung Ok Kim

    2013-01-01

    Full Text Available To study the effects of [6]-gingerol, a ginger phytochemical, on tight junction (TJ molecules, we investigated TJ tightening and signal transduction pathways in human pancreatic duct cell-derived cancer cell line PANC-1. The following methods were utilized: MTT assay to determine cytotoxicity; zymography to examine matrix metalloproteinase (MMP activities; transepithelial electrical resistance (TER and paracellular flux for TJ measurement; RT-PCR and immunoblotting for proteins related to TJ and invasion; and EMSA for NF-κB activity in PANC-1 cells. Results revealed that TER significantly increased and claudin 4 and MMP-9 decreased compared to those of the control. TJ protein levels, including zonula occludens (ZO- 1, occludin, and E-cadherin, increased in [6]-gingerol-treated cells, which correlated with a decrease in paracellular flux and MMP activity. Furthermore, NF-κB/Snail nuclear translocation was suppressed via downregulation of the extracellular signal-regulated kinase (ERK pathway in response to [6]-gingerol treatment. Moreover, treatment with U0126, an ERK inhibitor, completely blocked NF-κB activity. In conclusion, these findings demonstrate that [6]-gingerol regulates TJ-related proteins and suppresses invasion and metastasis through NF-κB/Snail inhibition via inhibition of the ERK pathway. Therefore, [6]-gingerol may suppress the invasive activity of PANC-1 cells.

  8. IL-4 and IL-13 Compromise the Sinonasal Epithelial Barrier and Perturb Intercellular Junction Protein Expression

    Science.gov (United States)

    Wise, Sarah K.; Laury, Adrienne M.; Katz, Elizabeth H.; Den Beste, Kyle A.; Parkos, Charles A.; Nusrat, Asma

    2014-01-01

    Introduction Altered expression of epithelial intercellular junction proteins has been observed in sinonasal biopsies from nasal polyps and epithelial layers cultured from nasal polyp patients. These alterations comprise a “leaky” epithelial barrier phenotype. We hypothesize that Th2 cytokines IL-4 and IL-13 modulate epithelial junction proteins thereby contributing to the leaky epithelial barrier. Methods Differentiated primary sinonasal epithelial layers cultured at the air-liquid interface were exposed to IL-4, IL-13, and controls for 24 hours at 37°C. Epithelial resistance measurements were taken every 4 hours during cytokine exposure. Western blot and immunofluorescence staining/confocal microscopy were used to assess changes in a panel of tight and adherens junction proteins. Western blot densitometry was quantified with image analysis. Results IL-4 and IL-13 exposure resulted in a mean decrease in transepithelial resistance at 24 hours to 51.6% (n=6) and 68.6% (n=8) of baseline, respectively. Tight junction protein JAM-A expression decreased 42.2% with IL-4 exposure (n=9) and 37.5% with IL-13 exposure (n=9). Adherens junction protein E-cadherin expression decreased 35.3% with IL-4 exposure (n=9) and 32.9% with IL-13 exposure (n=9). Tight junction protein claudin-2 showed more variability but had a trend toward higher expression with Th2 cytokine exposure. There were no appreciable changes in claudin-1, occludin, or ZO-1 with IL-4 or IL-13 exposure. Conclusion Sinonasal epithelial exposure to Th2 cytokines IL-4 and IL-13 results in alterations in intercellular junction proteins, reflecting increased epithelial permeability. Such changes may explain some of the phenotypic manifestations of Th2-mediated sinonasal disease, such as edema, nasal discharge, and environmental reactivity. PMID:24510479

  9. TJ-II Library Manual (Version 2)

    International Nuclear Information System (INIS)

    Tribaldos, V.; Milligen, B. Ph. van; Lopez-Fraguas, A.

    2001-01-01

    This is a manual of use of the TJ2 Numerical Library that has been developed for making numerical computations of different TJ-II configurations. This manual is a new version of the earlier manual CIEMAT report 806. (Author)

  10. Trends in drug delivery through tissue barriers containing tight junctions.

    Science.gov (United States)

    Tscheik, Christian; Blasig, Ingolf E; Winkler, Lars

    2013-04-01

    A limitation in the uptake of many drugs is the restricted permeation through tissue barriers. There are two general ways to cross barriers formed by cell layers: by transcytosis or by diffusion through the intercellular space. In the latter, tight junctions (TJs) play the decisive role in the regulation of the barrier permeability. Thus, transient modulation of TJs is a potent strategy to improve drug delivery. There have been extensive studies on surfactant-like absorption enhancers. One of the most effective enhancers found is sodium caprate. However, this modulates TJs in an unspecific fashion. A novel approach would be the specific modulation of TJ-associated marvel proteins and claudins, which are the main structural components of the TJs. Recent studies have identified synthetic peptidomimetics and RNA interference techniques to downregulate the expression of targeted TJ proteins. This review summarizes current progress and discusses the impact on TJs' barrier function.

  11. TJ-II Library Manual

    International Nuclear Information System (INIS)

    Tribaldos, V.; Milligen, B.Ph., van; Lopez-Fraguas, A.

    1996-01-01

    This report contains a detailed description of the TJ-II library and its routines. The library is written in FORTRAN 77 language and is available in the CRAY J916 and DEC Alpha 8400 computers at CIEMAT. This document also contains some examples of its use. (Author)

  12. Process of tight junction recovery in the injured vocal fold epithelium: Morphological and paracellular permeability analysis.

    Science.gov (United States)

    Suzuki, Ryo; Katsuno, Tatsuya; Kishimoto, Yo; Nakamura, Ryosuke; Mizuta, Masanobu; Suehiro, Atsushi; Yamashita, Masaru; Nakamura, Tatsuo; Tateya, Ichiro; Omori, Koichi

    2018-04-01

    The vocal fold epithelium that includes tight junction (TJ)-based barrier function protects underlying connective tissues from external insults. TJs play an important role to control paracellular permeability of not only solutes but also ions, and preserve the vocal fold homeostasis. However, the distribution of TJs and paracellular diffusion barrier across the entire vocal fold epithelium are still unknown. The aim of this study was to identify the distribution of TJs in the vocal fold epithelium and to characterize the recovery process of TJ-based paracellular diffusion barrier in a rat model of vocal fold injury. Animal experiments with controls. Normal and vocal fold-injured rats were used. Larynges were harvested for immunohistochemical examination of TJ proteins. For functional analysis, a tracer permeability assay was performed using EZ-Link Sulfo-NHS-LC-Biotin. TJ proteins occludin and zonula occludens 1 signals were localized to the junctional regions of the most luminal cell layers of the vocal fold epithelium. The injured region had been recovered with epithelium at 5 days postinjury, but the paracellular diffusion barrier assays revealed that biotinylation reagents diffused into the lamina propria at 5 days postinjury, and were blocked at the epithelium at 14 and 28 days postinjury. It was strongly suggested that TJs in the vocal fold epithelium exist at the junctional regions of the first layer of stratified squamous epithelium. TJ-based paracellular diffusion barrier following vocal fold injury is recovered by 14 days postinjury, and this period corresponds with the time course of structural changes in the regenerating epithelium layer. NA. Laryngoscope, 128:E150-E156, 2018. © 2017 The American Laryngological, Rhinological and Otological Society, Inc.

  13. Visualization and quantitative analysis of reconstituted tight junctions using localization microscopy.

    Directory of Open Access Journals (Sweden)

    Rainer Kaufmann

    Full Text Available Tight Junctions (TJ regulate paracellular permeability of tissue barriers. Claudins (Cld form the backbone of TJ-strands. Pore-forming claudins determine the permeability for ions, whereas that for solutes and macromolecules is assumed to be crucially restricted by the strand morphology (i.e., density, branching and continuity. To investigate determinants of the morphology of TJ-strands we established a novel approach using localization microscopy.TJ-strands were reconstituted by stable transfection of HEK293 cells with the barrier-forming Cld3 or Cld5. Strands were investigated at cell-cell contacts by Spectral Position Determination Microscopy (SPDM, a method of localization microscopy using standard fluorophores. Extended TJ-networks of Cld3-YFP and Cld5-YFP were observed. For each network, 200,000 to 1,100,000 individual molecules were detected with a mean localization accuracy of ∼20 nm, yielding a mean structural resolution of ∼50 nm. Compared to conventional fluorescence microscopy, this strongly improved the visualization of strand networks and enabled quantitative morphometric analysis. Two populations of elliptic meshes (mean diameter <100 nm and 300-600 nm, respectively were revealed. For Cld5 the two populations were more separated than for Cld3. Discrimination of non-polymeric molecules and molecules within polymeric strands was achieved. For both subtypes of claudins the mean density of detected molecules was similar and estimated to be ∼24 times higher within the strands than outside the strands.The morphometry and single molecule information provided advances the mechanistic analysis of paracellular barriers. Applying this novel method to different TJ-proteins is expected to significantly improve the understanding of TJ on the molecular level.

  14. Characterization of cytoskeletal and junctional proteins expressed by cells cultured from human arachnoid granulation tissue

    Directory of Open Access Journals (Sweden)

    Mehta Bhavya C

    2005-10-01

    Full Text Available Abstract Background The arachnoid granulations (AGs are projections of the arachnoid membrane into the dural venous sinuses. They function, along with the extracranial lymphatics, to circulate the cerebrospinal fluid (CSF to the systemic venous circulation. Disruption of normal CSF dynamics may result in increased intracranial pressures causing many problems including headaches and visual loss, as in idiopathic intracranial hypertension and hydrocephalus. To study the role of AGs in CSF egress, we have grown cells from human AG tissue in vitro and have characterized their expression of those cytoskeletal and junctional proteins that may function in the regulation of CSF outflow. Methods Human AG tissue was obtained at autopsy, and explanted to cell culture dishes coated with fibronectin. Typically, cells migrated from the explanted tissue after 7–10 days in vitro. Second or third passage cells were seeded onto fibronectin-coated coverslips at confluent densities and grown to confluency for 7–10 days. Arachnoidal cells were tested using immunocytochemical methods for the expression of several common cytoskeletal and junctional proteins. Second and third passage cultures were also labeled with the common endothelial markers CD-31 or VE-cadherin (CD144 and their expression was quantified using flow cytometry analysis. Results Confluent cultures of arachnoidal cells expressed the intermediate filament protein vimentin. Cytokeratin intermediate filaments were expressed variably in a subpopulation of cells. The cultures also expressed the junctional proteins connexin43, desmoplakin 1 and 2, E-cadherin, and zonula occludens-1. Flow cytometry analysis indicated that second and third passage cultures failed to express the endothelial cell markers CD31 or VE-cadherin in significant quantities, thereby showing that these cultures did not consist of endothelial cells from the venous sinus wall. Conclusion To our knowledge, this is the first report of

  15. Effect of Glucagon-like Peptide 2 on Tight Junction in Jejunal Epithelium of Weaned Pigs though MAPK Signaling Pathway

    Directory of Open Access Journals (Sweden)

    Changsong Yu

    2014-05-01

    Full Text Available The glucagon-like peptide 2 (GLP-2 that is expressed in intestine epithelial cells of mammals, is important for intestinal barrier function and regulation of tight junction (TJ proteins. However, there is little known about the intracellular mechanisms of GLP-2 in the regulation of TJ proteins in piglets’ intestinal epithelial cells. The purpose of this study is to test the hypothesis that GLP-2 regulates the expressions of TJ proteins in the mitogen-activated protein kinase (MAPK signaling pathway in piglets’ intestinal epithelial cells. The jejunal tissues were cultured in a Dulbecco’s modified Eagle’s medium/high glucose medium containing supplemental 0 to 100 nmol/L GLP-2. At 72 h after the treatment with the appropriate concentrations of GLP-2, the mRNA and protein expressions of zonula occludens-1 (ZO-1, occludin and claudin-1 were increased (p<0.05. U0126, an MAPK kinase inhibitor, prevented the mRNA and protein expressions of ZO-1, occludin, claudin-1 increase induced by GLP-2 (p<0.05. In conclusion, these results indicated that GLP-2 could improve the expression of TJ proteins in weaned pigs’ jejunal epithelium, and the underlying mechanism may due to the MAPK signaling pathway.

  16. Roles of neuro-exocytotic proteins at the neuromuscular junction

    NARCIS (Netherlands)

    Sons-Michel, Michèle S.

    2011-01-01

    The aim of the studies described in the thesis was to elucidate the roles of several neuro-exocytotic proteins at the motor nerve terminal in neuromuscular synaptic transmission, making use of genetic knockout (KO) mice, each missing one (or more) neuro-exocytotic proteins. In addition, it was

  17. Protein kinase C isoforms at the neuromuscular junction: localization and specific roles in neurotransmission and development.

    Science.gov (United States)

    Lanuza, Maria A; Santafe, Manel M; Garcia, Neus; Besalduch, Núria; Tomàs, Marta; Obis, Teresa; Priego, Mercedes; Nelson, Phillip G; Tomàs, Josep

    2014-01-01

    The protein kinase C family (PKC) regulates a variety of neural functions including neurotransmitter release. The selective activation of a wide range of PKC isoforms in different cells and domains is likely to contribute to the functional diversity of PKC phosphorylating activity. In this review, we describe the isoform localization, phosphorylation function, regulation and signalling of the PKC family at the neuromuscular junction. Data show the involvement of the PKC family in several important functions at the neuromuscular junction and in particular in the maturation of the synapse and the modulation of neurotransmission in the adult. © 2013 Anatomical Society.

  18. The gap junction protein connexin43 interacts with the second PDZ domain of the zona occludens-1 protein

    NARCIS (Netherlands)

    Giepmans, B N; Moolenaar, W H

    1998-01-01

    Gap junctions mediate cell-cell communication in almost all tissues and are composed of channel-forming integral membrane proteins, termed connexins [1-3]. Connexin43 (Cx43) is the most widely expressed and the most well-studied member of this family. Cx43-based cell-cell communication is regulated

  19. Expression of Tight Junction Protein Claudin-1 in Human Crescentic Glomerulonephritis

    Directory of Open Access Journals (Sweden)

    Ryo Koda

    2014-01-01

    Full Text Available The origin of crescent forming cells in human glomerulonephritis (GN remains unknown. Some animal studies demonstrated that parietal epithelial cells of Bowman’s capsule (PECs were the main component of proliferating cells and PEC-specific tight junction protein claudin-1 was expressed in crescentic lesions. We investigated the expression of claudin-1 in human GN. Immunohistochemistry for claudin-1 was performed on 17 kidney biopsy samples with crescent formation. Colocalization of claudin-1 with intracellular tight junction protein ZO-1 was also evaluated by immunofluorescence double staining. Claudin-1 is expressed mainly at the cell to cell contact site of proliferating cells in cellular crescentic lesions in patients with these forms of human GN. Small numbers of crescent forming cells showed extrajunctional localization of claudin-1. Colocalization of claudin-1 with ZO-1 was found at cell to cell contact sites of adjacent proliferating cells. In control samples, staining of claudin-1 was positive in PECs, but not in podocytes. Our findings suggest that claudin-1 contributes to crescent formation as a component of the tight junction protein complex that includes ZO-1. Co-localization of claudin-1 with ZO-1 implies the formation of functional tight junction complexes in crescentic lesions to prevent the interstitial damage caused by penetration of filtered molecules from Bowman’s space.

  20. Protein kinase C-dependent regulation of connexin43 gap junctions and hemichannels

    DEFF Research Database (Denmark)

    Alstrøm, Jette Skov; Stroemlund, Line Waring; Nielsen, Morten Schak

    2015-01-01

    Connexin43 (Cx43) generates intercellular gap junction channels involved in, among others, cardiac and brain function. Gap junctions are formed by the docking of two hemichannels from neighbouring cells. Undocked Cx43 hemichannels can upon different stimuli open towards the extracellular matrix...... and allow transport of molecules such as fluorescent dyes and ATP. A range of phosphorylated amino acids have been detected in the C-terminus of Cx43 and their physiological role has been intensively studied both in the gap junctional form of Cx43 and in its hemichannel configuration. We present the current...... knowledge of protein kinase C (PKC)-dependent regulation of Cx43 and discuss the divergent results....

  1. Evidence for differential changes of junctional complex proteins in murine neurocysticercosis dependent upon CNS vasculature.

    Science.gov (United States)

    Alvarez, Jorge I; Teale, Judy M

    2007-09-12

    The delicate balance required to maintain homeostasis of the central nervous system (CNS) is controlled by the blood-brain barrier (BBB). Upon injury, the BBB is disrupted compromising the CNS. BBB disruption has been represented as a uniform event. However, our group has shown in a murine model of neurocysticercosis (NCC) that BBB disruption varies depending upon the anatomical site/vascular bed analyzed. In this study further understanding of the mechanisms of BBB disruption was explored in blood vessels located in leptomeninges (pial vessels) and brain parenchyma (parenchymal vessels) by examining the expression of junctional complex proteins in murine brain infected with Mesocestoides corti. Both pial and parenchymal vessels from mock infected animals showed significant colocalization of junctional proteins and displayed an organized architecture. Upon infection, the patterned organization was disrupted and in some cases, particular tight junction and adherens junction proteins were undetectable or appeared to be undergoing proteolysis. The extent and timing of these changes differed between both types of vessels (pial vessel disruption within days versus weeks for parenchymal vessels). To approach potential mechanisms, the expression and activity of matrix metalloproteinase-9 (MMP-9) were evaluated by in situ zymography. The results indicated an increase in MMP-9 activity at sites of BBB disruption exhibiting leukocyte infiltration. Moreover, the timing of MMP activity in pial and parenchymal vessels correlated with the timing of permeability disruption. Thus, breakdown of the BBB is a mutable process despite the similar structure of the junctional complex between pial and parenchymal vessels and involvement of MMP activity.

  2. Phylogenetic and bioinformatic analysis of gap junction-related proteins, innexins, pannexins and connexins.

    Science.gov (United States)

    Fushiki, Daisuke; Hamada, Yasuo; Yoshimura, Ryoichi; Endo, Yasuhisa

    2010-04-01

    All multi-cellular animals, including hydra, insects and vertebrates, develop gap junctions, which communicate directly with neighboring cells. Gap junctions consist of protein families called connexins in vertebrates and innexins in invertebrates. Connexins and innexins have no homology in their amino acid sequence, but both are thought to have some similar characteristics, such as a tetra-membrane-spanning structure, formation of a channel by hexamer, and transmission of small molecules (e.g. ions) to neighboring cells. Pannexins were recently identified as a homolog of innexins in vertebrate genomes. Although pannexins are thought to share the function of intercellular communication with connexins and innexins, there is little information about the relationship among these three protein families of gap junctions. We phylgenetically and bioinformatically examined these protein families and other tetra-membrane-spanning proteins using a database and three analytical softwares. The clades formed by pannexin families do not belong to the species classification but do to paralogs of each member of pannexins. Amino acid sequences of pannexins are closely related to those of innexins but less to those of connexins. These data suggest that innexins and pannexins have a common origin, but the relationship between innexins/pannexins and connexins is as slight as that of other tetra-membrane-spanning members.

  3. Identification of MarvelD3 as a tight junction-associated transmembrane protein of the occludin family

    Directory of Open Access Journals (Sweden)

    Balda Maria S

    2009-12-01

    Full Text Available Abstract Background Tight junctions are an intercellular adhesion complex of epithelial and endothelial cells, and form a paracellular barrier that restricts the diffusion of solutes on the basis of size and charge. Tight junctions are formed by multiprotein complexes containing cytosolic and transmembrane proteins. How these components work together to form functional tight junctions is still not well understood and will require a complete understanding of the molecular composition of the junction. Results Here we identify a new transmembrane component of tight junctions: MarvelD3, a four-span transmembrane protein. Its predicted transmembrane helices form a Marvel (MAL and related proteins for vesicle traffic and membrane link domain, a structural motif originally discovered in proteins involved in membrane apposition and fusion events, such as the tight junction proteins occludin and tricellulin. In mammals, MarvelD3 is expressed as two alternatively spliced isoforms. Both isoforms exhibit a broad tissue distribution and are expressed by different types of epithelial as well as endothelial cells. MarvelD3 co-localises with occludin at tight junctions in intestinal and corneal epithelial cells. RNA interference experiments in Caco-2 cells indicate that normal MarvelD3 expression is not required for the formation of functional tight junctions but depletion results in monolayers with increased transepithelial electrical resistance. Conclusions Our data indicate that MarvelD3 is a third member of the tight junction-associated occludin family of transmembrane proteins. Similar to occludin, normal expression of MarvelD3 is not essential for the formation of functional tight junctions. However, MarvelD3 functions as a determinant of epithelial paracellular permeability properties.

  4. Tight junctions: a barrier to the initiation and progression of breast cancer?

    LENUS (Irish Health Repository)

    Brennan, Kieran

    2010-01-01

    Breast cancer is a complex and heterogeneous disease that arises from epithelial cells lining the breast ducts and lobules. Correct adhesion between adjacent epithelial cells is important in determining the normal structure and function of epithelial tissues, and there is accumulating evidence that dysregulated cell-cell adhesion is associated with many cancers. This review will focus on one cell-cell adhesion complex, the tight junction (TJ), and summarize recent evidence that TJs may participate in breast cancer development or progression. We will first outline the protein composition of TJs and discuss the functions of the TJ complex. Secondly we will examine how alterations in these functions might facilitate breast cancer initiation or progression; by focussing on the regulatory influence of TJs on cell polarity, cell fate and cell migration. Finally we will outline how pharmacological targeting of TJ proteins may be useful in limiting breast cancer progression. Overall we hope to illustrate that the relationship between TJ alterations and breast cancer is a complex one; but that this area offers promise in uncovering fundamental mechanisms linked to breast cancer progression.

  5. Tjæreborg Wind Turbine (Esbjerg)

    DEFF Research Database (Denmark)

    Øye, Stig

    1991-01-01

    This paper presents the first measured timeseries for the Tjæreborg (Tjaereborg) Wind Turbine during operation with stepwise pitch angle changes.......This paper presents the first measured timeseries for the Tjæreborg (Tjaereborg) Wind Turbine during operation with stepwise pitch angle changes....

  6. Indigenous lactobacilli strains of food and human sources reverse enteropathogenic E. coli O26:H11-induced damage in intestinal epithelial cell lines: effect on redistribution of tight junction proteins.

    Science.gov (United States)

    Jariwala, Ruchi; Mandal, Hemanti; Bagchi, Tamishraha

    2017-09-01

    The aim of the study was to investigate the neutralizing effect of lactobacilli isolated from indigenous food and human sources on enteropathogenic Escherichia coli (EPEC) O26 : H11-induced epithelial barrier dysfunction in vitro. This was assessed by transepithelial electrical resistance (TEER) and permeability assays using intestinal cell lines, HT-29 and Caco-2. Furthermore, the expression and distribution of tight junction (TJ) proteins were analysed by qRT-PCR and immunofluorescence assay, respectively. The nine strains used in the study were from different species viz. Lactobacillus fermentum, Lactobacillushelveticus, Lactobacillus salivarius and Lactobacillus plantarum. All strains were able to reverse the decrease in TEER and corresponding increase in permeability across E. coli-infected monolayers. Maximum reversal was observed after 18 h [up to 93.8±2.0 % by L. rhamnosus GG followed by L. fermentum IIs11.2 (92.6±2.2 %) and L. plantarum GRI-2 (91.9±0.9 %)] of lactobacilli exposure following EPEC O26 : H11 infection. All strains were able to redistribute the TJ proteins to the cell periphery either partially or completely. Moreover, L. helveticus FA-7 was also able to significantly increase the mRNA expression of ZO-1 and claudin-1 (2.5-fold and 3.0-fold, respectively; PGRI-2 were good in all the aspects studied, and the other strains were good in some aspects. L. helveticus FA-7, L. fermentum FA-1 and L. plantarum GRI-2 can therefore be used for potential therapeutic purpose against intestinal epithelial dysfunction.

  7. Effect of kamikihito (TJ-137) on paclitaxel-induced olfactory neuropathy in vivo

    International Nuclear Information System (INIS)

    Yamamoto, Junpei

    2012-01-01

    A Kampo product, kamikihito (product name code: TJ-137), has ingredients that promote nerve growth. Paclitaxel, a cancer chemotherapeutic agent, is toxic to olfactory nerve cells in vivo. We found that TJ-137 is effective in reducing paclitaxel induced olfactory neuropathy in vivo. Female 7-week-old Bulb/c mice were fed food containing TJ-137, or control food, for 14 days before and after intravenous paclitaxel administration. 201 Tl uptake in nasal turbinates of TJ-137 treated mice (n=8) and control mice (n=9) was assessed by gamma spectrometry 6 hrs after nasal administration of 201 Tl. The epithelial changes in the nasal turbinates of mice were assessed by H and E and immunohistochemical staining for the olfactory marker protein (OMP). The accumulation of the neuronal tracer (Dextran tetramethylrhodamine) in the olfactory bulb was assessed in frozen sections of mice 48 hrs after nasal administration of the tracer. The epithelium of nasal turbinates of TJ-137 treated mice was less injured than that of the control mice after paclitaxel administration. The nasal epithelium was significantly thicker in TJ-137 treated mice compared to control mice (P=0.019). The accumulation of the neuronal tracer in the olfactory bulb was higher in the TJ-137 treated mice compared to controls. 201 Tl uptake per weight in nasal turbinate of TJ-137 treated mice was significantly higher than that of control mice (P=0.008). Pre-medication with TJ-137 (kamikihito) was effective in increasing olfactory nerve viability after paclitaxel administration in vivo. (author)

  8. The Ly6 protein coiled is required for septate junction and blood brain barrier organisation in Drosophila.

    Directory of Open Access Journals (Sweden)

    Assia Hijazi

    Full Text Available BACKGROUND: Genetic analysis of the Drosophila septate junctions has greatly contributed to our understanding of the mechanisms controlling the assembly of these adhesion structures, which bear strong similarities with the vertebrate tight junctions and the paranodal septate junctions. These adhesion complexes share conserved molecular components and have a common function: the formation of paracellular barriers restraining the diffusion of solutes through epithelial and glial envelopes. METHODOLOGY/PRINCIPAL FINDINGS: In this work we characterise the function of the Drosophila cold gene, that codes for a protein belonging to the Ly6 superfamily of extracellular ligands. Analysis of cold mutants shows that this gene is specifically required for the organisation of the septate junctions in epithelial tissues and in the nervous system, where its contribution is essential for the maintenance of the blood-brain barrier. We show that cold acts in a cell autonomous way, and we present evidence indicating that this protein could act as a septate junction component. CONCLUSION/SIGNIFICANCE: We discuss the specific roles of cold and three other Drosophila members of the Ly6 superfamily that have been shown to participate in a non-redundant way in the process of septate junction assembly. We propose that vertebrate Ly6 proteins could fulfill analogous roles in tight junctions and/or paranodal septate junctions.

  9. The Ly6 protein coiled is required for septate junction and blood brain barrier organisation in Drosophila.

    Science.gov (United States)

    Hijazi, Assia; Haenlin, Marc; Waltzer, Lucas; Roch, Fernando

    2011-03-15

    Genetic analysis of the Drosophila septate junctions has greatly contributed to our understanding of the mechanisms controlling the assembly of these adhesion structures, which bear strong similarities with the vertebrate tight junctions and the paranodal septate junctions. These adhesion complexes share conserved molecular components and have a common function: the formation of paracellular barriers restraining the diffusion of solutes through epithelial and glial envelopes. In this work we characterise the function of the Drosophila cold gene, that codes for a protein belonging to the Ly6 superfamily of extracellular ligands. Analysis of cold mutants shows that this gene is specifically required for the organisation of the septate junctions in epithelial tissues and in the nervous system, where its contribution is essential for the maintenance of the blood-brain barrier. We show that cold acts in a cell autonomous way, and we present evidence indicating that this protein could act as a septate junction component. We discuss the specific roles of cold and three other Drosophila members of the Ly6 superfamily that have been shown to participate in a non-redundant way in the process of septate junction assembly. We propose that vertebrate Ly6 proteins could fulfill analogous roles in tight junctions and/or paranodal septate junctions.

  10. The effect of direct-fed microbial supplementation, as an alternative to antibiotics, on growth performance, intestinal immune status and epithelial barrier protein expression in broiler chickens

    Science.gov (United States)

    The objective of this study was to investigate the effects of Bacillus subtilis-based probiotic supplementation in broiler chicken diets on growth performance, feed efficiency, intestinal cytokine and tight junction (TJ) protein mRNA expression. Day-old broiler chicks (n = 140) were randomly assigne...

  11. The effects of direct-fed microbial supplementation, as alternative to antibiotics, on growth performance, intestinal immune status and epithelial barrier protein expression in broiler chickens

    Science.gov (United States)

    This study was conducted to investigate the effects of Bacillus subtilis supplementation in broiler chicken diets on growth performance, feed efficiency, intestinal cytokine and tight junction (TJ) protein mRNA expression. Day-old broiler chicks (n = 140) were assigned five dietary treatments: basal...

  12. Unique cell type-specific junctional complexes in vascular endothelium of human and rat liver sinusoids.

    Directory of Open Access Journals (Sweden)

    Cyrill Géraud

    Full Text Available Liver sinusoidal endothelium is strategically positioned to control access of fluids, macromolecules and cells to the liver parenchyma and to serve clearance functions upstream of the hepatocytes. While clearance of macromolecular debris from the peripheral blood is performed by liver sinusoidal endothelial cells (LSECs using a delicate endocytic receptor system featuring stabilin-1 and -2, the mannose receptor and CD32b, vascular permeability and cell trafficking are controlled by transcellular pores, i.e. the fenestrae, and by intercellular junctional complexes. In contrast to blood vascular and lymphatic endothelial cells in other organs, the junctional complexes of LSECs have not yet been consistently characterized in molecular terms. In a comprehensive analysis, we here show that LSECs express the typical proteins found in endothelial adherens junctions (AJ, i.e. VE-cadherin as well as α-, β-, p120-catenin and plakoglobin. Tight junction (TJ transmembrane proteins typical of endothelial cells, i.e. claudin-5 and occludin, were not expressed by rat LSECs while heterogenous immunreactivity for claudin-5 was detected in human LSECs. In contrast, junctional molecules preferentially associating with TJ such as JAM-A, B and C and zonula occludens proteins ZO-1 and ZO-2 were readily detected in LSECs. Remarkably, among the JAMs JAM-C was considerably over-expressed in LSECs as compared to lung microvascular endothelial cells. In conclusion, we show here that LSECs form a special kind of mixed-type intercellular junctions characterized by co-occurrence of endothelial AJ proteins, and of ZO-1 and -2, and JAMs. The distinct molecular architecture of the intercellular junctional complexes of LSECs corroborates previous ultrastructural findings and provides the molecular basis for further analyses of the endothelial barrier function of liver sinusoids under pathologic conditions ranging from hepatic inflammation to formation of liver metastasis.

  13. Altered expressions of endothelial junction protein of placental capillaries in premature infants with intraventricular hemorrhage

    Directory of Open Access Journals (Sweden)

    Maria Ekawati

    2016-10-01

    Full Text Available Background: Placental hypoxia may lead to oxidative stress, which inflicts damage to capillary protein junction. The aim of this study was to evaluate altered expression of endothelial junction protein of capillaries in hypoxia condition and to observe its correlation with the incidence of  intraventricular hemorrhage in premature infants.Methods: A cross-sectional study was conducted by using placental tissues of premature infants as amodel of capillary integrity (29 hypoxic and 29 non-hypoxic. Hypoxia inducible factor (HIF-1α was measured to define placental tissue response to hypoxia; malondialdehyde (MDA and glutathione (GSH served as markers of oxidative stress. The expressions of junctional proteins, N-cadherin and occludin were analyzed by immunohistochemistry. Intraventricular hemorrhage (IVH was detected by cranial ultrasound at the third day. Unpaired t test, Mann-Whitney, and Chi-square tests were used to analyze the data.Results: The HIF-1α and MDA levels were slightly, but not significantly, higher in hypoxia group {13.64±8.70 pg/mg protein and 10.31 pmol/mg tissue (ranged 1.92–93.61, respectively}  compared to non- hypoxia group {10.65±5.35 pg/mg protein and 9.77 pmol/mg tissue (ranged 2.42–93.31}. GSH levels were not different in both groups (38.14 (ranged 9.44–118.91 and  38.47(ranged 16.49–126.76 ng/mg protein, respectively. mRNA expression of N-cadherin (0.13 and occludin (0.096 were significantly lower in hypoxia comparedto non-hypoxia group (p=0,001, while protein expression of  N-cadherin (3.4; 75.9; 6.9; 13.8% and occludin  (20.7; 3.4; 69.0; 3.4; 6.9%  in hypoxia group was not associated with IVH (p=0.783 and p=0.743.Conclusion: Hypoxia altered expression of endothelial junction protein in placental capillaries, but no association with intraventricular hemorrhage was observed.

  14. The novel protein kinase C epsilon isoform modulates acetylcholine release in the rat neuromuscular junction.

    Science.gov (United States)

    Obis, Teresa; Hurtado, Erica; Nadal, Laura; Tomàs, Marta; Priego, Mercedes; Simon, Anna; Garcia, Neus; Santafe, Manel M; Lanuza, Maria A; Tomàs, Josep

    2015-12-01

    Various protein kinase C (PKC) isoforms contribute to the phosphorylating activity that modulates neurotransmitter release. In previous studies we showed that nPKCε is confined in the presynaptic site of the neuromuscular junction and its presynaptic function is activity-dependent. Furthermore, nPKCε regulates phorbol ester-induced acetylcholine release potentiation, which further indicates that nPKCε is involved in neurotransmission. The present study is designed to examine the nPKCε involvement in transmitter release at the neuromuscular junction. We use the specific nPKCε translocation inhibitor peptide εV1-2 and electrophysiological experiments to investigate the involvement of this isoform in acetylcholine release. We observed that nPKCε membrane translocation is key to the synaptic potentiation of NMJ, being involved in several conditions that upregulate PKC isoforms coupling to acetylcholine (ACh) release (incubation with high Ca(2+), stimulation with phorbol esters and protein kinase A, stimulation with adenosine 3',5'-cyclic monophosphorothioate, 8-Bromo-, Rp-isomer, sodium salt -Sp-8-BrcAMP-). In all these conditions, preincubation with the nPKCε translocation inhibitor peptide (εV1-2) impairs PKC coupling to acetylcholine release potentiation. In addition, the inhibition of nPKCε translocation and therefore its activity impedes that presynaptic muscarinic autoreceptors and adenosine autoreceptors modulate transmitter secretion. Together, these results point to the importance of nPKCε isoform in the control of acetylcholine release in the neuromuscular junction.

  15. Particulate matter air pollution disrupts endothelial cell barrier via calpain-mediated tight junction protein degradation

    Directory of Open Access Journals (Sweden)

    Wang Ting

    2012-08-01

    Full Text Available Abstract Background Exposure to particulate matter (PM is a significant risk factor for increased cardiopulmonary morbidity and mortality. The mechanism of PM-mediated pathophysiology remains unknown. However, PM is proinflammatory to the endothelium and increases vascular permeability in vitro and in vivo via ROS generation. Objectives We explored the role of tight junction proteins as targets for PM-induced loss of lung endothelial cell (EC barrier integrity and enhanced cardiopulmonary dysfunction. Methods Changes in human lung EC monolayer permeability were assessed by Transendothelial Electrical Resistance (TER in response to PM challenge (collected from Ft. McHenry Tunnel, Baltimore, MD, particle size >0.1 μm. Biochemical assessment of ROS generation and Ca2+ mobilization were also measured. Results PM exposure induced tight junction protein Zona occludens-1 (ZO-1 relocation from the cell periphery, which was accompanied by significant reductions in ZO-1 protein levels but not in adherens junction proteins (VE-cadherin and β-catenin. N-acetyl-cysteine (NAC, 5 mM reduced PM-induced ROS generation in ECs, which further prevented TER decreases and atteneuated ZO-1 degradation. PM also mediated intracellular calcium mobilization via the transient receptor potential cation channel M2 (TRPM2, in a ROS-dependent manner with subsequent activation of the Ca2+-dependent protease calpain. PM-activated calpain is responsible for ZO-1 degradation and EC barrier disruption. Overexpression of ZO-1 attenuated PM-induced endothelial barrier disruption and vascular hyperpermeability in vivo and in vitro. Conclusions These results demonstrate that PM induces marked increases in vascular permeability via ROS-mediated calcium leakage via activated TRPM2, and via ZO-1 degradation by activated calpain. These findings support a novel mechanism for PM-induced lung damage and adverse cardiovascular outcomes.

  16. Transport with Astra in TJ-II

    International Nuclear Information System (INIS)

    Lopez-Bruna, D.; Castejon, F.; Fontdecaba, J. M.

    2004-01-01

    This report describes the adaptation of the numerical transport shell ASTRA for performing plasma calculations in the TJ-II stellarator device. Firstly, an approximation to the TJ-II geometry is made and a simple transport model is shared with two other codes in order to compare these codes (PROCTR, PRETOR-Stellarator) with ASTRA as calculation tool for TJ-II plasmas are provided: interpretative and predictive transport. The first consists in estimating the transport coefficients from real experimental data, thes being taken from three TJ-II discharges. The predictive facet is illustrated using a model that is able to includes self-consistently the dynamics of transport barriers. The report includes this model, written in the ASTRA programming language, to illustrate the use of ASTRA. (Author) 26 refs

  17. Uterine inactivation of muscle segment homeobox (Msx) genes alters epithelial cell junction proteins during embryo implantation.

    Science.gov (United States)

    Sun, Xiaofei; Park, Craig B; Deng, Wenbo; Potter, S Steven; Dey, Sudhansu K

    2016-04-01

    Embryo implantation requires that the uterus differentiate into the receptive state. Failure to attain uterine receptivity will impede blastocyst attachment and result in a compromised pregnancy. The molecular mechanism by which the uterus transitions from the prereceptive to the receptive stage is complex, involving an intricate interplay of various molecules. We recently found that mice with uterine deletion ofMsxgenes (Msx1(d/d)/Msx2(d/d)) are infertile because of implantation failure associated with heightened apicobasal polarity of luminal epithelial cells during the receptive period. However, information on Msx's roles in regulating epithelial polarity remains limited. To gain further insight, we analyzed cell-type-specific gene expression by RNA sequencing of separated luminal epithelial and stromal cells by laser capture microdissection fromMsx1(d/d)/Msx2(d/d)and floxed mouse uteri on d 4 of pseudopregnancy. We found that claudin-1, a tight junction protein, and small proline-rich (Sprr2) protein, a major component of cornified envelopes in keratinized epidermis, were substantially up-regulated inMsx1(d/d)/Msx2(d/d)uterine epithelia. These factors also exhibited unique epithelial expression patterns at the implantation chamber (crypt) inMsx1(f/f)/Msx2(f/f)females; the patterns were lost inMsx1(d/d)/Msx2(d/d)epithelia on d 5, suggesting important roles during implantation. The results suggest thatMsxgenes play important roles during uterine receptivity including modulation of epithelial junctional activity.-Sun, X., Park, C. B., Deng, W., Potter, S. S., Dey, S. K. Uterine inactivation of muscle segment homeobox (Msx) genes alters epithelial cell junction proteins during embryo implantation. © FASEB.

  18. Dendrobium chrysotoxum Lindl. Alleviates Diabetic Retinopathy by Preventing Retinal Inflammation and Tight Junction Protein Decrease

    Science.gov (United States)

    Yu, Zengyang; Gong, Chenyuan; Lu, Bin; Yang, Li; Sheng, Yuchen; Ji, Lili; Wang, Zhengtao

    2015-01-01

    Diabetic retinopathy (DR) is a serious complication of diabetes mellitus. This study aimed to observe the alleviation of the ethanol extract of Dendrobium chrysotoxum Lindl. (DC), a traditional Chinese herbal medicine, on DR and its engaged mechanism. After DC (30 or 300 mg/kg) was orally administrated, the breakdown of blood retinal barrier (BRB) in streptozotocin- (STZ-) induced diabetic rats was attenuated by DC. Decreased retinal mRNA expression of tight junction proteins (including occludin and claudin-1) in diabetic rats was also reversed by DC. Western blot analysis and retinal immunofluorescence staining results further confirmed that DC reversed the decreased expression of occludin and claudin-1 proteins in diabetic rats. DC reduced the increased retinal mRNA expressions of intercellular adhesion molecule-1 (ICAM-1), tumor necrosis factor α (TNFα), interleukin- (IL-) 6, and IL-1β in diabetic rats. In addition, DC alleviated the increased 1 and phosphorylated p65, IκB, and IκB kinase (IKK) in diabetic rats. DC also reduced the increased serum levels of TNFα, interferon-γ (IFN-γ), IL-6, IL-1β, IL-8, IL-12, IL-2, IL-3, and IL-10 in diabetic rats. Therefore, DC can alleviate DR by inhibiting retinal inflammation and preventing the decrease of tight junction proteins, such as occludin and claudin-1. PMID:25685822

  19. Petri Net-Based Model of Helicobacter pylori Mediated Disruption of Tight Junction Proteins in Stomach Lining during Gastric Carcinoma

    Directory of Open Access Journals (Sweden)

    Anam Naz

    2017-09-01

    Full Text Available Tight junctions help prevent the passage of digestive enzymes and microorganisms through the space between adjacent epithelial cells lining. However, Helicobacter pylori encoded virulence factors negatively regulate these tight junctions and contribute to dysfunction of gastric mucosa. Here, we have predicted the regulation of important tight junction proteins, such as Zonula occludens-1, Claudin-2 and Connexin32 in the presence of pathogenic proteins. Molecular events such as post translational modifications and crosstalk between phosphorylation, O-glycosylation, palmitoylation and methylation are explored which may compromise the integrity of these tight junction proteins. Furthermore, the signaling pathways disrupted by dysregulated kinases, proteins and post-translational modifications are reviewed to design an abstracted computational model showing the situation-dependent dynamic behaviors of these biological processes and entities. A qualitative hybrid Petri Net model is therefore constructed showing the altered host pathways in the presence of virulence factor cytotoxin-associated gene A, leading to the disruption of tight junction proteins. The model is qualitative logic-based, which does not depend on any kinetic parameter and quantitative data and depends on knowledge derived from experiments. The designed model provides insights into the tight junction disruption and disease progression. Model is then verified by the available experimental data, nevertheless formal in vitro experimentation is a promising way to ensure its validation. The major findings propose that H. pylori activated kinases are responsible to trigger specific post translational modifications within tight junction proteins, at specific sites. These modifications may favor alterations in gastric barrier and provide a route to bacterial invasion into host cells.

  20. Estrogen decreases tight junction protein ZO-1 expression in human primary gut tissues.

    Science.gov (United States)

    Zhou, Zejun; Zhang, Lumin; Ding, Miao; Luo, Zhenwu; Yuan, Shao; Bansal, Meena B; Gilkeson, Gary; Lang, Ren; Jiang, Wei

    2017-10-01

    Females have a higher prevalence of most autoimmune diseases; however, the mechanism is unknown. In this study, we examined the expression of tight junction protein zonula occludens 1 (ZO-1) and estrogen receptor (ER)-α/β in human primary gut tissues by immunohistochemistry, immunofluorescence and qPCR. The expression of ZO-1 and ER-β but not ER-α was present in both male and female gut tissues. There was no sex difference in ER-β expression, but ZO-1 expression was decreased in females compared to males. In vitro, estrogen treatment decreased ZO-1 mRNA and protein expression, ZO-1 promoter activity, IL-6 production, and NF-κB activation in human primary gut tissues or the Caco-2 cells, but increased the ER-β expression in Caco-2 cells. Consistently, plasma IL-6 levels in females were reduced relative to males in vivo. Our finding indicates that estrogen may play a role in gut tight junction expression and permeability. Copyright © 2017 Elsevier Inc. All rights reserved.

  1. Junction region of EWS-FLI1 fusion protein has a dominant negative effect in Ewing's sarcoma in vitro.

    Science.gov (United States)

    Jully, Babu; Vijayalakshmi, Ramshankar; Gopal, Gopisetty; Sabitha, Kesavan; Rajkumar, Thangarajan

    2012-11-12

    Ewing's sarcoma is a malignancy characterized by a specific 11:22 chromosomal translocation which generates a novel EWS-FLI1 fusion protein functioning as an aberrant transcription factor. In the present study, we have further characterized the junction region of the EWS-FLI1 fusion protein. In-silico model of EWS-FLI1 fusion protein was analysed for ligand binding sites, and a putative region (amino acid (aa) 251-343 of the type 1 fusion protein) in the vicinity of the fusion junction was cloned and expressed using bacterial expression. The recombinant protein was characterized by Circular Dichroism (CD). We then expressed aa 251-280 ectopically in Ewing's sarcoma cell-line and its effect on cell proliferation, tumorigenicity and expression of EWS-FLI1 target genes were analysed. Our modelling analysis indicated that Junction region (aa 251-343) encompasses potential ligand biding sites in the EWS-FLI1 protein and when expressed in bacteria was present as soluble form. Ectopically expressing this region in Ewing's sarcoma cells inhibited tumorigenicity, and EWS-FLI1 target genes indicating a dominant negative biological effect. Junction region can be exploited further as target for drug development in future to specifically target EWS-FLI1 in Ewing's Sarcoma.

  2. Intestinal epithelial barrier function and tight junction proteins with heat and exercise

    DEFF Research Database (Denmark)

    Dokladny, Karol; Zuhl, Micah N; Moseley, Pope L

    2016-01-01

    A single layer of enterocytes and tight junctions (intercellular multiprotein complexes) form the intestinal epithelial barrier that controls transport of molecules through transcellular and paracellular pathways. A dysfunctional or "leaky" intestinal tight junction barrier allows augmented perme...

  3. Tjæreborg Wind Turbine

    DEFF Research Database (Denmark)

    Øye, Stig

    1991-01-01

    This paper presents results from the fourth measurement camapign at the Tjæreborg (Tjaereborg) WInd Turbine during operation with stepwise pitch angle changes. The measurements cover one hour of operation at wind speeds between 7 and 10 m/s aceraging approximately 8.7 m/s.......This paper presents results from the fourth measurement camapign at the Tjæreborg (Tjaereborg) WInd Turbine during operation with stepwise pitch angle changes. The measurements cover one hour of operation at wind speeds between 7 and 10 m/s aceraging approximately 8.7 m/s....

  4. Claudin-4 Overexpression in Epithelial Ovarian Cancer Is Associated with Hypomethylation and Is a Potential Target for Modulation of Tight Junction Barrier Function Using a C-Terminal Fragment of Clostridium perfringens Enterotoxin

    Directory of Open Access Journals (Sweden)

    Babak Litkouhi

    2007-04-01

    Full Text Available BACKGROUND: Claudin-4, a tight junction (TJ protein and receptor for the C-terminal fragment of Clostridium perfringens enterotoxin (C-CPE, is overexpressed in epithelial ovarian cancer (EOC. Previous research suggests DNA methylation is a mechanism for claudin-4 overexpression in cancer and that C-CPE acts as an absorption-enhancing agent in claudin-4expressing cells. We sought to correlate claudin-4 overexpression in EOC with clinical outcomes and TJ barrier function, investigate DNA methylation as a mechanism for overexpression, and evaluate the effect of C-CPE on the TJ. METHODS: Claudin-4 expression in EOC was quantified and correlated with clinical outcomes. Claudin-4 methylation status was determined, and claudin-4-negative cell lines were treated with a demethylating agent. Electric cell-substrate impedance sensing was used to calculate junctional (paracellular resistance (Rb in EOC cells after claudin-4 silencing and after C-CPE treatment. RESULTS: Claudin4 overexpression in EOC does not correlate with survival or other clinical endpoints and is associated with hypomethylation. Claudin-4 overexpression correlates with Rb and C-CPE treatment of EOC cells significantly decreased Rb in a dose- and claudin-4-dependent noncytotoxic manner. CONCLUSIONS: C-CPE treatment of EOC cells leads to altered TJ function. Further research is needed to determine the potential clinical applications of C-CPE in EOC drug delivery strategies.

  5. Dioscorin protects tight junction protein expression in A549 human airway epithelium cells from dust mite damage.

    Science.gov (United States)

    Fu, Lin Shien; Ko, Ying Hsien; Lin, Kuo Wei; Hsu, Jeng Yuan; Chu, Jao Jia; Chi, Chin Shiang

    2009-12-01

    In addition to being an allergen, the trypsin activity of dust mite extract also destroys the tight junctions of bronchial epithelium. Such damage can lead to airway leakage, which increases airway exposure to allergens, irritants, and other pathogens. Dioscorin, the storage protein of yam, demonstrates anti-trypsin activity, as well as other potential anti-inflammatory effects. This study investigated the protective role of dioscorin for tight junctions. The immunofluorescence stains of zonula occludens (ZO-1), E-cadherin (EC) and desmoplakin (DP) proteins were compared. A cultured A549 cell line was used as a control and A549 cells were incubated with mite extract 100 mg/mL for 16 h, with or without dioscorin 100 mg/mL pretreatment for 8 h and with dioscorin 100 mg/mL alone for 16 h. Western blot was performed to detect changes in ZO-1, EC, and DP in the treated A549 cell lines. Loss of tight junction protein expression (ZO-1, EC, DP) was demonstrated after 16-h mite extract incubation. The defect could be restored if cells were pretreated with dioscorin for 8 h. In addition, dioscorin did not cause damage to the A549 cell lines in terms of cell survival or morphology. Western blot showed no change in the amount of tight junction protein under various conditions. Dioscorin is a potential protector of airway damage caused by mite extract.

  6. Transport with Astra in TJ-II; Transporte con Astra en TJ-II

    Energy Technology Data Exchange (ETDEWEB)

    Lopez-Bruna, D; Castejon, F; Fontdecaba, J M

    2004-07-01

    This report describes the adaptation of the numerical transport shell ASTRA for performing plasma calculations in the TJ-II stellarator device. Firstly, an approximation to the TJ-II geometry is made and a simple transport model is shared with two other codes in order to compare these codes (PROCTR, PRETOR-Stellarator) with ASTRA as calculation tool for TJ-II plasmas are provided: interpretative and predictive transport. The first consists in estimating the transport coefficients from real experimental data, thes being taken from three TJ-II discharges. The predictive facet is illustrated using a model that is able to includes self-consistently thedynamics of transport barriers. The report includes this model, written in the ASTRA programming language, to illustrate the use of ASTRA. (Author) 26 refs.

  7. Eps homology domain endosomal transport proteins differentially localize to the neuromuscular junction

    Directory of Open Access Journals (Sweden)

    Mate Suzanne E

    2012-09-01

    Full Text Available Abstract Background Recycling of endosomes is important for trafficking and maintenance of proteins at the neuromuscular junction (NMJ. We have previously shown high expression of the endocytic recycling regulator Eps15 homology domain-containing (EHD1 proteinin the Torpedo californica electric organ, a model tissue for investigating a cholinergic synapse. In this study, we investigated the localization of EHD1 and its paralogs EHD2, EHD3, and EHD4 in mouse skeletal muscle, and assessed the morphological changes in EHD1−/− NMJs. Methods Localization of the candidate NMJ protein EHD1 was assessed by confocal microscopy analysis of whole-mount mouse skeletal muscle fibers after direct gene transfer and immunolabeling. The potential function of EHD1 was assessed by specific force measurement and α-bungarotoxin-based endplate morphology mapping in EHD1−/− mouse skeletal muscle. Results Endogenous EHD1 localized to primary synaptic clefts of murine NMJ, and this localization was confirmed by expression of recombinant green fluorescent protein labeled-EHD1 in murine skeletal muscle in vivo. EHD1−/− mouse skeletal muscle had normal histology and NMJ morphology, and normal specific force generation during muscle contraction. The EHD 1–4 proteins showed differential localization in skeletal muscle: EHD2 to muscle vasculature, EHD3 to perisynaptic regions, and EHD4 to perinuclear regions and to primary synaptic clefts, but at lower levels than EHD1. Additionally, specific antibodies raised against mammalian EHD1-4 recognized proteins of the expected mass in the T. californica electric organ. Finally, we found that EHD4 expression was more abundant in EHD1−/− mouse skeletal muscle than in wild-type skeletal muscle. Conclusion EHD1 and EHD4 localize to the primary synaptic clefts of the NMJ. Lack of obvious defects in NMJ structure and muscle function in EHD1−/− muscle may be due to functional compensation by other EHD paralogs.

  8. The t-J model at small t/j: Numerical, perturbative, and supersymmetric results

    International Nuclear Information System (INIS)

    Barnes, T.; Tennessee Univ., Knoxville, TN

    1991-02-01

    We discuss some recent results for one- and two-hole states in the t-J model at small t/J. These include numerical results (bandwidth determinations and accurate t/J values for 4 x 4 lattice one-hole ground-state level crossings), hopping-parameter perturbation theory (which gives the small-t/J one-hole bandwidth in terms of the static-vacancy ground state), and results at the supersymmetric point t/J = 1/2 (exact results for energies and bandwidths.) The perturbative results leads us to a new conjecture regarding the staggered magnetization of higher-spin states in the two-dimensional Heisenberg model. We also discuss extrapolation of small-t/J results to high-T c parameter values; in the two-hole ground states we find (t/J) λ behavior in the rms hole-hole separation, and an extrapolation to t/J = 3 gives a bulk-limit rms hole-hole separation of ∼ 7 angstrom. 18 refs., 6 figs

  9. Proinflammatory cytokines tumor necrosis factor-alpha and interferon-gamma alter tight junction structure and function in the rat parotid gland Par-C10 cell line.

    Science.gov (United States)

    Baker, Olga J; Camden, Jean M; Redman, Robert S; Jones, Jonathan E; Seye, Cheikh I; Erb, Laurie; Weisman, Gary A

    2008-11-01

    Sjögren's syndrome (SS) is an autoimmune disorder characterized by inflammation and dysfunction of salivary glands, resulting in impaired secretory function. The production of the proinflammatory cytokines tumor necrosis factor-alpha (TNF-alpha) and interferon-gamma (IFN-gamma) is elevated in exocrine glands of patients with SS, although little is known about the effects of these cytokines on salivary epithelial cell functions necessary for saliva secretion, including tight junction (TJ) integrity and the establishment of transepithelial ion gradients. The present study demonstrates that chronic exposure of polarized rat parotid gland (Par-C10) epithelial cell monolayers to TNF-alpha and IFN-gamma decreases transepithelial resistance (TER) and anion secretion, as measured by changes in short-circuit current (I(sc)) induced by carbachol, a muscarinic cholinergic receptor agonist, or UTP, a P2Y(2) nucleotide receptor agonist. In contrast, TNF-alpha and IFN-gamma had no effect on agonist-induced increases in the intracellular calcium concentration [Ca(2+)](i) in Par-C10 cells. Furthermore, treatment of Par-C10 cell monolayers with TNF-alpha and IFN-gamma increased paracellular permeability to normally impermeant proteins, altered cell and TJ morphology, and downregulated the expression of the TJ protein, claudin-1, but not other TJ proteins expressed in Par-C10 cells. The decreases in TER, agonist-induced transepithelial anion secretion, and claudin-1 expression caused by TNF-alpha, but not IFN-gamma, were reversible by incubation of Par-C10 cell monolayers with cytokine-free medium for 24 h, indicating that IFN-gamma causes irreversible inhibition of cellular activities associated with fluid secretion in salivary glands. Our results suggest that cytokine production is an important contributor to secretory dysfunction in SS by disrupting TJ integrity of salivary epithelium.

  10. A role for tight junction-associated MARVEL proteins in larval sea lamprey (Petromyzon marinus) osmoregulation.

    Science.gov (United States)

    Kolosov, Dennis; Bui, Phuong; Donini, Andrew; Wilkie, Mike P; Kelly, Scott P

    2017-10-15

    This study reports on tight junction-associated MARVEL proteins of larval sea lamprey ( Petromyzon marinus ) and their potential role in ammocoete osmoregulation. Two occludin isoforms (designated Ocln and Ocln-a) and a tricellulin (Tric) were identified. Transcripts encoding ocln , ocln-a and tric were broadly expressed in larval lamprey, with the greatest abundance of ocln in the gut, liver and kidney, ocln-a in the gill and skin, and tric in the kidney. Ocln and Ocln-a resolved as ∼63 kDa and ∼35 kDa MW proteins, respectively, while Tric resolved as a ∼50 kDa protein. Ocln immunolocalized to the gill vasculature and in gill mucous cells while Ocln-a localized to the gill pouch and gill epithelium. Both Ocln and Ocln-a localized in the nephron, the epidermis and the luminal side of the gut. In branchial tissue, Tric exhibited punctate localization, consistent with its presence at regions of tricellular contact. Following ion-poor water (IPW) acclimation of ammocoetes, serum [Na + ] and [Cl - ] decreased, but not [Ca 2+ ], and carcass moisture content increased. In association, Ocln abundance increased in the skin and kidney, but reduced in the gill of IPW-acclimated ammocoetes while Ocln-a abundance reduced in the kidney only. Tric abundance increased in the gill. Region-specific alterations in ocln , ocln-a and tric mRNA abundance were also observed in the gut. Data support a role for Ocln, Ocln-a and Tric in the osmoregulatory strategies of a basal vertebrate. © 2017. Published by The Company of Biologists Ltd.

  11. Oncostatin M induces upregulation of claudin-2 in rodent hepatocytes coinciding with changes in morphology and function of tight junctions

    International Nuclear Information System (INIS)

    Imamura, Masafumi; Kojima, Takashi; Lan, Mengdong; Son, Seiichi; Murata, Masaki; Osanai, Makoto; Chiba, Hideki; Hirata, Koichi; Sawada, Norimasa

    2007-01-01

    In rodent livers, integral tight junction (TJ) proteins claudin-1, -2, -3, -5 and -14 are detected and play crucial roles in the barrier to keep bile in bile canaculi away from the blood circulation. Claudin-2 shows a lobular gradient increasing from periportal to pericentral hepatocytes, whereas claudin-1 and -3 are expressed in the whole liver lobule. Although claudin-2 expression induces cation-selective channels in tight junctions of epithelial cells, the physiological functions and regulation of claudin-2 in hepatocytes remain unclear. Oncostatin M (OSM) is a multifunctional cytokine implicated in the differentiation of hepatocytes that induces formation of E-cadherin-based adherens junctions in fetal hepatocytes. In this study, we examined whether OSM could induce expression and function of claudin-2 in rodent hepatocytes, immortalized mouse and primary cultured proliferative rat hepatocytes. In the immortalized mouse and primary cultured proliferative rat hepatocytes, treatment with OSM markedly increased mRNA and protein of claudin-2 together with formation of developed networks of TJ strands. The increase of claudin-2 enhanced the paracellular barrier function which depended on molecular size. The increase of claudin-2 expression induced by OSM in rodent hepatocytes was regulated through distinct signaling pathways including PKC. These results suggest that expression of claudin-2 in rodent hepatocytes may play a specific role as controlling the size of paracellular permeability in the barrier to keep bile in bile canaculi

  12. TJ-II operation tracking from Cadarache

    International Nuclear Information System (INIS)

    Vega, J.; Sanchez, E.; Portas, A.; Pereira, A.; Lopez, A.; Ascasibar, E.; Balme, S.; Buravand, Y.; Lebourg, P.; Theis, J.M.; Utzel, N.; Ruiz, M.; Barrera, E.; Lopez, S.; Machon, D.; Castro, R.; Lopez, D.; Mollinedo, A.; Munoz, J.A.

    2005-01-01

    The TJ-II remote participation system was designed to follow the TJ-II discharge production, even allowing the physicist in charge of operation to be in a remote location. The system has been based on both web servers and Java technology. These elements were chosen due to its open character, security properties, platform independency and technological maturity. Web pages and Java applications permit users to access experimental systems, data servers and the operation logbook. Security resources are provided by the PAPI system, a distributed authentication and authorization system. The TJ-II remote participation tools have allowed us to command and follow the stellarator operation from Cadarache. Over 1,000 digitizer channels and more than 20 diagnostic control systems were remotely available from web pages for monitoring/programming purposes. One Java application provided on-line information about the acquisition status of channels and acquisition cards. A second Java application showed temporal evolution signals that were refreshed in an automated way on the screen after each shot. A third Java application provided access to the operation logbook. In addition to these tools, we used the VRVS videoconferencing system (FUSION community, X-Point room) and the EFDA Messenger Service for instant messaging (Jabber client). (author)

  13. TJ-II operation tracking from Cadarache

    International Nuclear Information System (INIS)

    Vega, J.; Sanchez, E.; Portas, A.; Pereira, A.; Lopez, A.; Ascasibar, E.

    2005-01-01

    The TJ-II remote participation system was designed to follow the TJ-II discharge production, even allowing the physicist in charge of operation to be in a remote location. The system has been based on both web servers and Java technology. These elements were chosen due to its open character, security properties, platform independence and technological maturity. Web pages and Java applications permit users to access experimental systems, data servers and the operation logbook. Security resources are provided by the PAPI system, a distributed authentication and authorization system. The TJ-II remote participation tools have allowed us to command and follow the stellarator operation from Cadarache. Over 1,000 digitizer channels and more than 20 diagnostic control systems were remotely available from web pages for monitoring/programming purposes. One Java application provided on-line information about the acquisition status of channels and acquisition cards. A second Java application showed temporal evolution signals that were refreshed in an automated way on the screen after each shot. A third Java application provided access to the operation logbook. In addition to these tools, we used the VRVS video conferencing system (FUSION community, X-Point room) and the EFDA Messenger Service for instant messaging (Jabber client). (Author)

  14. Leptospira interrogans causes quantitative and morphological disturbances in adherens junctions and other biological groups of proteins in human endothelial cells.

    Science.gov (United States)

    Sato, Hiromi; Coburn, Jenifer

    2017-07-01

    Pathogenic Leptospira transmits from animals to humans, causing the zoonotic life-threatening infection called leptospirosis. This infection is reported worldwide with higher risk in tropical regions. Symptoms of leptospirosis range from mild illness to severe illness such as liver damage, kidney failure, respiratory distress, meningitis, and fatal hemorrhagic disease. Invasive species of Leptospira rapidly disseminate to multiple tissues where this bacterium damages host endothelial cells, increasing vascular permeability. Despite the burden in humans and animals, the pathogenic mechanisms of Leptospira infection remain to be elucidated. The pathogenic leptospires adhere to endothelial cells and permeabilize endothelial barriers in vivo and in vitro. In this study, human endothelial cells were infected with the pathogenic L. interrogans serovar Copenhageni or the saprophyte L. biflexa serovar Patoc to investigate morphological changes and other distinctive phenotypes of host cell proteins by fluorescence microscopy. Among those analyzed, 17 proteins from five biological classes demonstrated distinctive phenotypes in morphology and/or signal intensity upon infection with Leptospira. The affected biological groups include: 1) extracellular matrix, 2) intercellular adhesion molecules and cell surface receptors, 3) intracellular proteins, 4) cell-cell junction proteins, and 5) a cytoskeletal protein. Infection with the pathogenic strain most profoundly disturbed the biological structures of adherens junctions (VE-cadherin and catenins) and actin filaments. Our data illuminate morphological disruptions and reduced signals of cell-cell junction proteins and filamentous actin in L. interrogans-infected endothelial cells. In addition, Leptospira infection, regardless of pathogenic status, influenced other host proteins belonging to multiple biological classes. Our data suggest that this zoonotic agent may damage endothelial cells via multiple cascades or pathways

  15. Leptospira interrogans causes quantitative and morphological disturbances in adherens junctions and other biological groups of proteins in human endothelial cells.

    Directory of Open Access Journals (Sweden)

    Hiromi Sato

    2017-07-01

    Full Text Available Pathogenic Leptospira transmits from animals to humans, causing the zoonotic life-threatening infection called leptospirosis. This infection is reported worldwide with higher risk in tropical regions. Symptoms of leptospirosis range from mild illness to severe illness such as liver damage, kidney failure, respiratory distress, meningitis, and fatal hemorrhagic disease. Invasive species of Leptospira rapidly disseminate to multiple tissues where this bacterium damages host endothelial cells, increasing vascular permeability. Despite the burden in humans and animals, the pathogenic mechanisms of Leptospira infection remain to be elucidated. The pathogenic leptospires adhere to endothelial cells and permeabilize endothelial barriers in vivo and in vitro. In this study, human endothelial cells were infected with the pathogenic L. interrogans serovar Copenhageni or the saprophyte L. biflexa serovar Patoc to investigate morphological changes and other distinctive phenotypes of host cell proteins by fluorescence microscopy. Among those analyzed, 17 proteins from five biological classes demonstrated distinctive phenotypes in morphology and/or signal intensity upon infection with Leptospira. The affected biological groups include: 1 extracellular matrix, 2 intercellular adhesion molecules and cell surface receptors, 3 intracellular proteins, 4 cell-cell junction proteins, and 5 a cytoskeletal protein. Infection with the pathogenic strain most profoundly disturbed the biological structures of adherens junctions (VE-cadherin and catenins and actin filaments. Our data illuminate morphological disruptions and reduced signals of cell-cell junction proteins and filamentous actin in L. interrogans-infected endothelial cells. In addition, Leptospira infection, regardless of pathogenic status, influenced other host proteins belonging to multiple biological classes. Our data suggest that this zoonotic agent may damage endothelial cells via multiple cascades or

  16. Leptospira interrogans causes quantitative and morphological disturbances in adherens junctions and other biological groups of proteins in human endothelial cells

    Science.gov (United States)

    Sato, Hiromi

    2017-01-01

    Pathogenic Leptospira transmits from animals to humans, causing the zoonotic life-threatening infection called leptospirosis. This infection is reported worldwide with higher risk in tropical regions. Symptoms of leptospirosis range from mild illness to severe illness such as liver damage, kidney failure, respiratory distress, meningitis, and fatal hemorrhagic disease. Invasive species of Leptospira rapidly disseminate to multiple tissues where this bacterium damages host endothelial cells, increasing vascular permeability. Despite the burden in humans and animals, the pathogenic mechanisms of Leptospira infection remain to be elucidated. The pathogenic leptospires adhere to endothelial cells and permeabilize endothelial barriers in vivo and in vitro. In this study, human endothelial cells were infected with the pathogenic L. interrogans serovar Copenhageni or the saprophyte L. biflexa serovar Patoc to investigate morphological changes and other distinctive phenotypes of host cell proteins by fluorescence microscopy. Among those analyzed, 17 proteins from five biological classes demonstrated distinctive phenotypes in morphology and/or signal intensity upon infection with Leptospira. The affected biological groups include: 1) extracellular matrix, 2) intercellular adhesion molecules and cell surface receptors, 3) intracellular proteins, 4) cell-cell junction proteins, and 5) a cytoskeletal protein. Infection with the pathogenic strain most profoundly disturbed the biological structures of adherens junctions (VE-cadherin and catenins) and actin filaments. Our data illuminate morphological disruptions and reduced signals of cell-cell junction proteins and filamentous actin in L. interrogans-infected endothelial cells. In addition, Leptospira infection, regardless of pathogenic status, influenced other host proteins belonging to multiple biological classes. Our data suggest that this zoonotic agent may damage endothelial cells via multiple cascades or pathways

  17. First plasmas in the TJ-II flexible Heliac

    International Nuclear Information System (INIS)

    Alejaldre, C.; Alonso, J.; Almoguera, L.

    1999-01-01

    First plasmas have been successfully achieved in the TJ-II stellarator using electron cyclotron resonance heating (f = 53.2 GHz, P ECRH = 250 kW). Initial experiments have explored the TJ-II flexibility in a wide range of plasma volumes, different rotational transform and magnetic well values. In this paper, the main results of this campaign are presented and, in particular, the influence of plasma wall interaction phenomena on TJ-II operation is discussed briefly. (author)

  18. Impact of obesity on 7,12-dimethylbenz[a]anthracene-induced altered ovarian connexin gap junction proteins in female mice

    International Nuclear Information System (INIS)

    Ganesan, Shanthi; Nteeba, Jackson; Keating, Aileen F.

    2015-01-01

    The ovarian gap junction proteins alpha 4 (GJA4 or connexin 37; CX37), alpha 1 (GJA1 or connexin 43; CX43) and gamma 1 (GJC1 or connexin 45; CX45) are involved in cell communication and folliculogenesis. 7,12-dimethylbenz[a]anthracene (DMBA) alters Cx37 and Cx43 expression in cultured neonatal rat ovaries. Additionally, obesity has an additive effect on DMBA-induced ovarian cell death and follicle depletion, thus, we investigated in vivo impacts of obesity and DMBA on CX protein levels. Ovaries were collected from lean and obese mice aged 6, 12, 18, or 24 wks. A subset of 18 wk old mice (lean and obese) were dosed with sesame oil or DMBA (1 mg/kg; ip) for 14 days and ovaries collected 3 days thereafter. Cx43 and Cx45 mRNA and protein levels decreased (P < 0.05) after 18 wks while Cx37 mRNA and protein levels decreased (P < 0.05) after 24 wks in obese ovaries. Cx37 mRNA and antral follicle protein staining intensity were reduced (P < 0.05) by obesity while total CX37 protein was reduced (P < 0.05) in DMBA exposed obese ovaries. Cx43 mRNA and total protein levels were decreased (P < 0.05) by DMBA in both lean and obese ovaries while basal protein staining intensity was reduced (P < 0.05) in obese controls. Cx45 mRNA, total protein and protein staining intensity level were decreased (P < 0.05) by obesity. These data support that obesity temporally alters gap junction protein expression and that DMBA-induced ovotoxicity may involve reduced gap junction protein function. - Highlights: • Ovarian gap junction proteins are affected by ovarian aging and obesity. • DMBA exposure negatively impacts gap junction proteins. • Altered gap junction proteins may contribute to infertility

  19. Impact of obesity on 7,12-dimethylbenz[a]anthracene-induced altered ovarian connexin gap junction proteins in female mice

    Energy Technology Data Exchange (ETDEWEB)

    Ganesan, Shanthi, E-mail: shanthig@iastate.edu; Nteeba, Jackson, E-mail: nteeba@iastate.edu; Keating, Aileen F., E-mail: akeating@iastate.edu

    2015-01-01

    The ovarian gap junction proteins alpha 4 (GJA4 or connexin 37; CX37), alpha 1 (GJA1 or connexin 43; CX43) and gamma 1 (GJC1 or connexin 45; CX45) are involved in cell communication and folliculogenesis. 7,12-dimethylbenz[a]anthracene (DMBA) alters Cx37 and Cx43 expression in cultured neonatal rat ovaries. Additionally, obesity has an additive effect on DMBA-induced ovarian cell death and follicle depletion, thus, we investigated in vivo impacts of obesity and DMBA on CX protein levels. Ovaries were collected from lean and obese mice aged 6, 12, 18, or 24 wks. A subset of 18 wk old mice (lean and obese) were dosed with sesame oil or DMBA (1 mg/kg; ip) for 14 days and ovaries collected 3 days thereafter. Cx43 and Cx45 mRNA and protein levels decreased (P < 0.05) after 18 wks while Cx37 mRNA and protein levels decreased (P < 0.05) after 24 wks in obese ovaries. Cx37 mRNA and antral follicle protein staining intensity were reduced (P < 0.05) by obesity while total CX37 protein was reduced (P < 0.05) in DMBA exposed obese ovaries. Cx43 mRNA and total protein levels were decreased (P < 0.05) by DMBA in both lean and obese ovaries while basal protein staining intensity was reduced (P < 0.05) in obese controls. Cx45 mRNA, total protein and protein staining intensity level were decreased (P < 0.05) by obesity. These data support that obesity temporally alters gap junction protein expression and that DMBA-induced ovotoxicity may involve reduced gap junction protein function. - Highlights: • Ovarian gap junction proteins are affected by ovarian aging and obesity. • DMBA exposure negatively impacts gap junction proteins. • Altered gap junction proteins may contribute to infertility.

  20. Wetting and premelting of triple junctions and grain boundaries in the Al-Zn alloys

    International Nuclear Information System (INIS)

    Straumal, B.; Kogtenkova, O.; Protasova, S.; Mazilkin, A.; Zieba, P.; Czeppe, T.; Wojewoda-Budka, J.; Faryna, M.

    2008-01-01

    Phase transitions in grain boundaries (GBs) and GB triple junctions (TJs) can change drastically the properties of polycrystals. The GB and TJ wetting phase transition can occur in the two-phase area of the bulk phase diagram where the liquid and solid phases are in equilibrium. The GB and TJ wetting tie-lines can continue in one-phase area of the bulk phase diagram as a GB or TJ solidus line. This line represents the GB or TJ premelting phase transition. The structure and composition of grain boundaries and GB triple junctions were studied by high-resolution electron microscopy and analytical transmission electron microscopy in the Al-5 at.% Zn polycrystals and by differential scanning calorimetry (DSC) in the Al-7.5 at.% Zn polycrystals. Between bulk solidus and GB or TJ solidus the metastable Zn-rich β m -phase was observed in the GB triple junctions of quenched samples. This phase appears neither in the samples annealed above the bulk solidus nor in those annealed below the GB solidus. Zn-content in this β m -phase corresponds to that of bulk liquidus. This is a structural indication that if the melt wets the GBs or TJs, the GB (or TJ) solidus line appears in the bulk phase diagram, and the liquid-like phase exists in GBs and TJs between bulk solidus and GB (or TJ) solidus lines. The structural observation of this phase is also supported by our data obtained by means of DSC

  1. Effects of topical steroids on tight junction proteins and spongiosis in esophageal epithelia of patients with eosinophilic esophagitis.

    Science.gov (United States)

    Katzka, David A; Tadi, Ravikanth; Smyrk, Thomas C; Katarya, Eesha; Sharma, Anamay; Geno, Deborah M; Camilleri, Michael; Iyer, Prasad G; Alexander, Jeffrey A; Buttar, Navtej S

    2014-11-01

    The allergic response associated with eosinophilic esophagitis (EoE) occurs when food antigens permeate tight junction-mediated epithelial dilated intercellular spaces. We assessed whether levels of tight junction proteins correlate with the dilation of intercellular spaces (spongiosis) and the effects of topical steroids on these parameters. We assessed esophageal biopsy samples from 10 patients with active EoE treated with topical fluticasone, 10 untreated patients, and 10 patients without esophageal disease (controls) for degree of spongiosis. Immunohistochemical assays were used to determine the levels of the tight junction proteins filaggrin, zonula occludens (ZO)-1, ZO-2, ZO-3, and claudin-1. Histology and immunohistochemistry results were assessed blindly, with levels of tight junction proteins and degree of spongiosis rated on scales of 0 to 3. The mean degrees of spongiosis in untreated and treated patients with EoE were 1.3 and 0.4, respectively (P = .016). Esophageal epithelia did not stain significantly for ZO-1 or ZO-2. Filaggrin was observed in a predominant cytoplasmic pattern, compared with the cytoplasmic and membranous patterns of ZO-3 and claudin-1. In biopsy specimens from patients with active EoE, the mean staining intensities for filaggrin, ZO-3, and claudin-1 were 1.6, 1.4, and 0.7, respectively. In biopsy specimens from patients treated with fluticasone, levels of filaggrin, ZO-3, and claudin-1 were 2.8 (P = .002 compared with untreated patients), 1.7 (P = .46 compared with untreated patients), and 1.3 (P = .25 compared with untreated patients), respectively. The correlation between the level of filaggrin and the degree of spongiosis was r = 0.23, and between ZO-3 staining and the degree of spongiosis was r = .016 (P = .001 for filaggrin vs ZO-3 staining). Filaggrin, ZO-3, and claudin-1 (but not ZO-1 or ZO-2) are detected in the esophageal mucosa of patients with EoE treated with steroids and individuals without esophageal disease

  2. Overview of TJ-II experiments

    International Nuclear Information System (INIS)

    Sanchez, J.; Acedo, M.; Alonso, A.

    2007-01-01

    This paper presents an overview of experimental results and progress made in investigating the link between magnetic topology, electric fields and transport in the TJ-II stellarator. The smooth change from positive to negative electric field observed in the core region as the density is raised is correlated with global and local transport data. A statistical description of transport is emerging as a new way to describe the coupling between profiles, plasma flows and turbulence. TJ-II experiments show that the location of rational surfaces inside the plasma can, in some circumstances, provide a trigger for the development of core transitions, providing a critical test for the various models that have been proposed to explain the appearance of transport barriers in relation to magnetic topology. In the plasma core, perpendicular rotation is strongly coupled to plasma density, showing a reversal consistent with neoclassical expectations. In contrast, spontaneous sheared flows in the plasma edge appear to be coupled strongly to plasma turbulence, consistent with the expectation for turbulent driven flows. The local injection of hydrocarbons through a mobile limiter and the erosion produced by plasmas with well-known edge parameters opens the possibility of performing carbon transport studies, relevant for understanding co-deposit formation in fusion devices

  3. Oligomeric structure and functional characterization of Caenorhabditis elegans Innexin-6 gap junction protein.

    Science.gov (United States)

    Oshima, Atsunori; Matsuzawa, Tomohiro; Nishikawa, Kouki; Fujiyoshi, Yoshinori

    2013-04-12

    Innexin is the molecular component of invertebrate gap junctions. Here we successfully expressed and purified Caenorhabditis elegans innexin-6 (INX-6) gap junction channels and characterized the molecular dimensions and channel permeability using electron microscopy (EM) and microinjection of fluorescent dye tracers, respectively. Negative staining and thin-section EM of isolated INX-6 gap junction membranes revealed a loosely packed hexagonal lattice and a greater cross-sectional width than that of connexin26 and connexin43 (Cx43)-GFP. In gel filtration analysis, the elution profile of purified INX-6 channels in dodecyl maltoside solution exhibited a peak at ∼400 kDa that was shifted to ∼800 kDa in octyl glucose neopentyl glycol. We also obtained the class averages of purified INX-6 channels from these peak fractions by single particle analysis. The class average from the ∼800-kDa fraction showed features of the junction form with a longitudinal height of 220 Å, a channel diameter of 110 Å in the absence of detergent micelles, and an extracellular gap space of 60 Å, whereas the class averages from the ∼400-kDa fraction showed diameters of up to 140 Å in the presence of detergent micelles. These findings indicate that the purified INX-6 channels are predominantly hemichannels in dodecyl maltoside and docked junction channels in octyl glucose neopentyl glycol. Dye transfer experiments revealed that the INX-6-GFP-His channels are permeable to 3- and 10-kDa tracers, whereas no significant amounts of these tracers passed through the Cx43-GFP channels. Based on these findings, INX-6 channels have a larger overall structure and greater permeability than connexin channels.

  4. Oligomeric Structure and Functional Characterization of Caenorhabditis elegans Innexin-6 Gap Junction Protein*

    Science.gov (United States)

    Oshima, Atsunori; Matsuzawa, Tomohiro; Nishikawa, Kouki; Fujiyoshi, Yoshinori

    2013-01-01

    Innexin is the molecular component of invertebrate gap junctions. Here we successfully expressed and purified Caenorhabditis elegans innexin-6 (INX-6) gap junction channels and characterized the molecular dimensions and channel permeability using electron microscopy (EM) and microinjection of fluorescent dye tracers, respectively. Negative staining and thin-section EM of isolated INX-6 gap junction membranes revealed a loosely packed hexagonal lattice and a greater cross-sectional width than that of connexin26 and connexin43 (Cx43)-GFP. In gel filtration analysis, the elution profile of purified INX-6 channels in dodecyl maltoside solution exhibited a peak at ∼400 kDa that was shifted to ∼800 kDa in octyl glucose neopentyl glycol. We also obtained the class averages of purified INX-6 channels from these peak fractions by single particle analysis. The class average from the ∼800-kDa fraction showed features of the junction form with a longitudinal height of 220 Å, a channel diameter of 110 Å in the absence of detergent micelles, and an extracellular gap space of 60 Å, whereas the class averages from the ∼400-kDa fraction showed diameters of up to 140 Å in the presence of detergent micelles. These findings indicate that the purified INX-6 channels are predominantly hemichannels in dodecyl maltoside and docked junction channels in octyl glucose neopentyl glycol. Dye transfer experiments revealed that the INX-6-GFP-His channels are permeable to 3- and 10-kDa tracers, whereas no significant amounts of these tracers passed through the Cx43-GFP channels. Based on these findings, INX-6 channels have a larger overall structure and greater permeability than connexin channels. PMID:23460640

  5. Remote Control of TJ-II Diagnostics

    International Nuclear Information System (INIS)

    Lopez Sanchez, A.; Vega, J.; Montoro, A.; Encabo, J.

    2001-01-01

    The present paper is about the design and development of ten remote control diagnostic systems used in the study of plasma fusion in the TJ-II device installed at CIEMAT. This development goes from the definition of sensors and devices necessary in carrying out these remote controls, to its assembly, wiring, development of electronic circuits inserted between sensors and PLC, development of programs for these PLC, connections and administration of the real time automation network, and later development of the necessary programs via the appropriate software tools for web access through a navigator to a specific web page, allowing visual and real time access over the auxiliary systems that make up all the diagnostics. (Author)

  6. Elucidating the principles of the molecular organization of heteropolymeric tight junction strands

    OpenAIRE

    Piontek, Jörg; Fritzsche, Susanne; Cording, Jimmi; Richter, Sandra; Hartwig, Jens; Walter, Maria; Yu, Dan; Turner, Jerrold R.; Gehring, Claudia; Rahn, Hans-Peter; Wolburg, Hartwig; Blasig, Ingolf E.

    2011-01-01

    Paracellular barrier properties of tissues are mainly determined by the composition of claudin hetero-polymers. To analyze the molecular organization of tight junctions (TJ), we investigated the ability of claudins (Cld) to form homo- and heteromers. Cld1, -2, -3, -5, and -12 expressed in cerebral barriers were investigated. TJ-strands were reconstituted by claudin-transfection of HEK293-cells. cis-Interactions and/or spatial proximity were analyzed by fluorescence resonance energy transfer i...

  7. Present status of the TJ-II remote participation system

    International Nuclear Information System (INIS)

    Vega, J.; Sanchez, E.; Lopez, A.; Portas, A.; Ochando, M.; Ascasibar, E.; Mollinedo, A.; Munoz, J.; Sanchez, A.; Ruiz, M.; Barrera, E.; Lopez, S.; Castro, R.; Lopez, D.

    2005-01-01

    The TJ-II remote participation system (RPS) was designed to extend to Internet the working capabilities provided in the TJ-II local environment, i.e., tracking the TJ-II operation, monitoring/programming data acquisition and control systems, and accessing databases. The TJ-II RPS was based on web and Java technologies because of their open character, security properties and technological maturity. A web server acts as a communication front-end between remote participants and local TJ-II elements. From the server side, web services are provided by means of resources supplied by JSP pages. The client part makes use of web browsers and ad hoc Java applications. The operation requires the use of a distributed authentication and authorization system. This development employs the PAPI System. At present, approximately 1000 digitisation channels can be managed from the TJ-II RPS. Furthermore, processing software based on a 4GL language (LabView) can be downloaded to multiprocessor data acquisition systems. Also, 15 diagnostic control systems, databases and the operation logbook are available from the RPS. The system even allows for the physicist in charge of operation to be in a remote location. Four Spanish universities make use of the TJ-II remote participation system capabilities for joint collaborations: these are the Universidad Politecnica de Madrid (UPM), Universidad Nacional de Educacion a Distancia (UNED), Universidad Complutense de Madrid (UCM) and Universidad Politecnica de Cataluna (UPC)

  8. Overview of TJ-II flexible heliac results

    Energy Technology Data Exchange (ETDEWEB)

    Ascasibar, E. E-mail: enrique.ascasibar@ciemat.es; Alejaldre, C.; Alonso, J.; Almoguera, L.; Baciero, A.; Balbin, R.; Blaumoser, M.; Botija, J.; Branas, B.; Cal, E. de la; Cappa, A.; Castellano, J.; Carrasco, R.; Castejon, F.; Cepero, J.R.; Cremy, C.; Doncel, J.; Eguilior, S.; Estrada, T.; Fernandez, A.; Fuentes, C.; Garcia, A.; Garcia-Cortes, I.; Guasp, J.; Herranz, J.; Hidalgo, C.; Jimenez, J.A.; Kirpitchev, I.; Krivenski, V.; Labrador, I.; Lapayese, F.; Likin, K.; Liniers, M.; Lopez-Fraguas, A.; Lopez-Sanchez, A.; Luna, E. de la; Martin, R.; Martinez-Laso, L.; Medrano, M.; Mendez, P.; McCarthy, K.J.; Medina, F.; Milligen, B. van; Ochando, M.; Pacios, L.; Pastor, I.; Pedrosa, M.A.; Pena, A. de la; Portas, A.; Qin, J.; Rodriguez-Rodrigo, L.; Romero, J.; Salas, A.; Sanchez, E.; Sanchez, J.; Tabares, F.; Tafalla, D.; Tribaldos, V.; Vega, J.; Zurro, B

    2001-10-01

    The TJ-II is a four period, low magnetic shear stellarator, with high degree of configuration flexibility (rotational transform from 0.9 to 2.5) which has been operating in Madrid since 1998 (R=1.5 m, a<0.22 m, B{sub 0}=1 T, P{sub ECRH}{<=}600 kW, P{sub NBI}{<=}3 MW under installation). This paper reviews the main technical aspects of the TJ-II heliac as well as the principal physics results obtained in the most recent TJ-II experimental campaign carried out in 2000.

  9. Overview of TJ-II flexible heliac results

    International Nuclear Information System (INIS)

    Ascasibar, E.; Alejaldre, C.; Alonso, J.; Almoguera, L.; Baciero, A.; Balbin, R.; Blaumoser, M.; Botija, J.; Branas, B.; Cal, E. de la; Cappa, A.; Castellano, J.; Carrasco, R.; Castejon, F.; Cepero, J.R.; Cremy, C.; Doncel, J.; Eguilior, S.; Estrada, T.; Fernandez, A.; Fuentes, C.; Garcia, A.; Garcia-Cortes, I.; Guasp, J.; Herranz, J.; Hidalgo, C.; Jimenez, J.A.; Kirpitchev, I.; Krivenski, V.; Labrador, I.; Lapayese, F.; Likin, K.; Liniers, M.; Lopez-Fraguas, A.; Lopez-Sanchez, A.; Luna, E. de la; Martin, R.; Martinez-Laso, L.; Medrano, M.; Mendez, P.; McCarthy, K.J.; Medina, F.; Milligen, B. van; Ochando, M.; Pacios, L.; Pastor, I.; Pedrosa, M.A.; Pena, A. de la; Portas, A.; Qin, J.; Rodriguez-Rodrigo, L.; Romero, J.; Salas, A.; Sanchez, E.; Sanchez, J.; Tabares, F.; Tafalla, D.; Tribaldos, V.; Vega, J.; Zurro, B.

    2001-01-01

    The TJ-II is a four period, low magnetic shear stellarator, with high degree of configuration flexibility (rotational transform from 0.9 to 2.5) which has been operating in Madrid since 1998 (R=1.5 m, a 0 =1 T, P ECRH ≤600 kW, P NBI ≤3 MW under installation). This paper reviews the main technical aspects of the TJ-II heliac as well as the principal physics results obtained in the most recent TJ-II experimental campaign carried out in 2000

  10. The effect of platelet rich plasma from bone marrow aspirate with added bone morphogenetic protein-2 on the Achilles tendon-bone junction in rabbits.

    Science.gov (United States)

    Kim, Hak Jun; Nam, Hyok-Woo; Hur, Chang-Yong; Park, Misu; Yang, Hee Seok; Kim, Byung-Soo; Park, Jung-Ho

    2011-12-01

    To determine if exogenously injected bone marrow derived platelet-rich plasma (PRP) plus bone morphogenetic protein (BMP)-2 could accelerate the healing of bone-tendon junction injuries and increase the junction holding strength during the early regeneration period. A direct injury model of the bone-tendon junction was made using an Achilles tendon-calcaneus bone junction in a rabbit. In the PRP/BMP-2/fibrin group, 0.05 mL of bone marrow derived PRP and 100 ng/mL of BMP-2 both incorporated into 0.1 mL of fibrin glue were injected into Achilles tendon-calcaneus bone junctions. The effect of the intervention was tested by comparing the results of an intervention group to a control group. The results of biomechanical testing, and histological and gross analyses were compared between the 2 groups at the following time points after surgery: 2 weeks, 4 weeks, and 8 weeks. Histologic examinations showed that woven bone developed in tendon-bone junctions at 2 weeks after surgery in the PRP/BMP-2/fibrin group. Mechanical test results showed no significant difference between the PRP/BMP-2/fibrin and control groups at 2 and 4 weeks after surgery, but the mean maximal load in the PRP/BMP-2/fibrin group was significantly higher than in the control group (p rabbit model of tendon-bone junction injury.

  11. Junction region of EWS-FLI1 fusion protein has a dominant negative effect in Ewing’s Sarcoma in vitro

    International Nuclear Information System (INIS)

    Jully, Babu; Vijayalakshmi, Ramshankar; Gopal, Gopisetty; Sabitha, Kesavan; Rajkumar, Thangarajan

    2012-01-01

    Ewing’s sarcoma is a malignancy characterized by a specific 11:22 chromosomal translocation which generates a novel EWS-FLI1 fusion protein functioning as an aberrant transcription factor. In the present study, we have further characterized the junction region of the EWS-FLI1 fusion protein. In-silico model of EWS-FLI1 fusion protein was analysed for ligand binding sites, and a putative region (amino acid (aa) 251–343 of the type 1 fusion protein) in the vicinity of the fusion junction was cloned and expressed using bacterial expression. The recombinant protein was characterized by Circular Dichroism (CD). We then expressed aa 251–280 ectopically in Ewing’s sarcoma cell-line and its effect on cell proliferation, tumorigenicity and expression of EWS-FLI1 target genes were analysed. Our modelling analysis indicated that Junction region (aa 251–343) encompasses potential ligand biding sites in the EWS-FLI1 protein and when expressed in bacteria was present as soluble form. Ectopically expressing this region in Ewing’s sarcoma cells inhibited tumorigenicity, and EWS-FLI1 target genes indicating a dominant negative biological effect. Junction region can be exploited further as target for drug development in future to specifically target EWS-FLI1 in Ewing’s Sarcoma

  12. Microfluidic chips with multi-junctions: an advanced tool in recovering proteins from inclusion bodies.

    Science.gov (United States)

    Yamaguchi, Hiroshi; Miyazaki, Masaya

    2015-01-01

    Active recombinant proteins are used for studying the biological functions of genes and for the development of therapeutic drugs. Overexpression of recombinant proteins in bacteria often results in the formation of inclusion bodies, which are protein aggregates with non-native conformations. Protein refolding is an important process for obtaining active recombinant proteins from inclusion bodies. However, the conventional refolding method of dialysis or dilution is time-consuming and recovered active protein yields are often low, and a cumbersome trial-and-error process is required to achieve success. To circumvent these difficulties, we used controllable diffusion through laminar flow in microchannels to regulate the denaturant concentration. This method largely aims at reducing protein aggregation during the refolding procedure. This Commentary introduces the principles of the protein refolding method using microfluidic chips and the advantage of our results as a tool for rapid and efficient recovery of active recombinant proteins from inclusion bodies.

  13. Confinement studies in the TJ-II stellarator

    NARCIS (Netherlands)

    Alejaldre, C.; Alonso, J.; Almoguera, L.; Ascasibar, E.; Baciero, A.; Balbin, R.; Blaumoser, M.; Botija, J.; Branas, B.; De La Cal, E.; Cappa, A.; Carrasco, R.; Castejon, F.; Cepero, J. R.; Cremy, C.; Delgrado, J. M.; Doncel, J.; Dulya, C.; Estrada, T.; Fernandez, A.; Fuentes, C.; Garcia, A.; Garcia-Cortes, I.; Guasp, J.; Herranz, J.; Hidalgo, C.; Jimenez, J. A.; Kirpitchev, I.; Krivenski, V.; Labrador, I.; Lapayese, F.; Likin, K.; Linier, M.; Lopez-Fraguas, A.; Lopez-Sanchez, A.; de la Luna, E.; Martin, R.; Martinez, A.; Martinez-Laso, L.; Medrano, M.; Mendez, P.; McCarthy, K. J.; Medina, F.; van Milligen, B.; Ochando, M.; Pacios, L.; Pastor, I.; Pedrosa, M. A.; de la Pena, A.; Portas, A.; Qin, J.; Rodriguez-Rodrigo, L.; Salas, A.; Sanchez, E.; Sanchez, J.; Tabares, F.; Tafalla, D.; Tribaldos, V.; Vega, J.; Zurro, B.; Akulina, D.; Fedyanin, O. I.; Grebenshchikov, S.; Kharchev, N.; Meshcheryakov, A.; Sarksian, K. A.; Barth, R.; van Dijk, G.; van der Meiden, H.

    1999-01-01

    ECR (electron cyclotron resonance) heated plasmas have been studied in the low magnetic shear TJ-II stellarator (R = 1.5 m, a < 0.22 m, B = 1 T, f = 53.2 GHz, P-ECRH = 300 kW, power density = 1-25 W cm(-3)). Recent experiments have explored the flexibility of the TJ-II across a wide range of

  14. Type 2 innate lymphoid cells disrupt bronchial epithelial barrier integrity by targeting tight junctions through IL-13 in asthmatic patients.

    Science.gov (United States)

    Sugita, Kazunari; Steer, Catherine A; Martinez-Gonzalez, Itziar; Altunbulakli, Can; Morita, Hideaki; Castro-Giner, Francesc; Kubo, Terufumi; Wawrzyniak, Paulina; Rückert, Beate; Sudo, Katsuko; Nakae, Susumu; Matsumoto, Kenji; O'Mahony, Liam; Akdis, Mübeccel; Takei, Fumio; Akdis, Cezmi A

    2018-01-01

    Bronchial epithelial barrier leakiness and type 2 innate lymphoid cells (ILC2s) have been separately linked to asthma pathogenesis; however, the influence of ILC2s on the bronchial epithelial barrier has not been investigated previously. We investigated the role of ILC2s in the regulation of bronchial epithelial tight junctions (TJs) and barrier function both in bronchial epithelial cells of asthmatic patients and healthy subjects and general innate lymphoid cell- and ILC2-deficient mice. Cocultures of human ILC2s and bronchial epithelial cells were used to determine transepithelial electrical resistance, paracellular flux, and TJ mRNA and protein expressions. The effect of ILC2s on TJs was examined by using a murine model of IL-33-induced airway inflammation in wild-type, recombination-activating gene 2 (Rag2) -/- , Rag2 -/- Il2rg -/- , and Rora sg/sg mice undergoing bone marrow transplantation to analyze the in vivo relevance of barrier disruption by ILC2s. ILC2s significantly impaired the epithelial barrier, as demonstrated by reduced transepithelial electrical resistance and increased fluorescein isothiocyanate-dextran permeability in air-liquid interface cultures of human bronchial epithelial cells. This was in parallel to decreased mRNAs and disrupted protein expression of TJ proteins and was restored by neutralization of IL-13. Intranasal administration of recombinant IL-33 to wild-type and Rag2 -/- mice lacking T and B cells triggered TJ disruption, whereas Rag2 -/- Il2rg -/- and Rora sg/sg mice undergoing bone marrow transplantation that lack ILC2s did not show any barrier leakiness. Direct nasal administration of IL-13 was sufficient to induce deficiency in the TJ barrier in the bronchial epithelium of mice in vivo. These data highlight an essential mechanism in asthma pathogenesis by demonstrating that ILC2s are responsible for bronchial epithelial TJ barrier leakiness through IL-13. Copyright © 2017 American Academy of Allergy, Asthma & Immunology

  15. Tight junction regulates epidermal calcium ion gradient and differentiation

    International Nuclear Information System (INIS)

    Kurasawa, Masumi; Maeda, Tetsuo; Oba, Ai; Yamamoto, Takuya; Sasaki, Hiroyuki

    2011-01-01

    Research highlights: → We disrupted epidermal tight junction barrier in reconstructed epidermis. → It altered Ca 2+ distribution and consequentially differentiation state as well. → Tight junction should affect epidermal homeostasis by maintaining Ca 2+ gradient. -- Abstract: It is well known that calcium ions (Ca 2+ ) induce keratinocyte differentiation. Ca 2+ distributes to form a vertical gradient that peaks at the stratum granulosum. It is thought that the stratum corneum (SC) forms the Ca 2+ gradient since it is considered the only permeability barrier in the skin. However, the epidermal tight junction (TJ) in the granulosum has recently been suggested to restrict molecular movement to assist the SC as a secondary barrier. The objective of this study was to clarify the contribution of the TJ to Ca 2+ gradient and epidermal differentiation in reconstructed human epidermis. When the epidermal TJ barrier was disrupted by sodium caprate treatment, Ca 2+ flux increased and the gradient changed in ion-capture cytochemistry images. Alterations of ultrastructures and proliferation/differentiation markers revealed that both hyperproliferation and precocious differentiation occurred regionally in the epidermis. These results suggest that the TJ plays a crucial role in maintaining epidermal homeostasis by controlling the Ca 2+ gradient.

  16. Overview of TJ-II experiments

    International Nuclear Information System (INIS)

    Alejaldre, C.; Alonso, J.; Almoguera, L.

    2005-01-01

    This paper presents an overview of experimental results and progress made in investigating the role of magnetic configuration on stability and transport in the TJ-II stellarator. Global confinement studies have revealed a positive dependence of energy confinement on the rotational transform and plasma density, together with different parametric dependences for metallic and boronised wall conditions. Spontaneous and biasing-induced improved confinement transitions, with some characteristics that resemble those of previously reported H-mode regimes in other stellarator devices, have been observed. Also, magnetic configuration scan experiments have shown an interplay between magnetic structure (rationals, magnetic shear), transport and electric fields. Although the DC radial electric fields are comparable with those expected from neoclassical calculations, additional mechanisms based on neoclassical/turbulent bifurcations and kinetic effects are needed to explain the impact of magnetic topology on flows and radial electric fields. Local transport studies have demonstrated a dependence of plasma diffusivities and convective velocities on plasma density and heating power in consistency with global confinement properties. Hydrocarbon fuelling experiments in configurations with a low order rational value in the rotational transform located in the proximity of the last close flux surface (n = 4/m = 2) have shown the impurity screening properties related to the expected divertor effect. First experiments in NBI plasmas are reported. (author)

  17. Reduced-droop green III-nitride light-emitting diodes utilizing GaN tunnel junction

    Science.gov (United States)

    Alhassan, Abdullah I.; Young, Erin C.; Alyamani, Ahmed Y.; Albadri, Abdulrahman; Nakamura, Shuji; DenBaars, Steven P.; Speck, James S.

    2018-04-01

    We report the fabrication of low-droop high-efficiency green c-plane light-emitting diodes (LEDs) utilizing GaN tunnel junction (TJ) contacts. The LED epitaxial layers with a top p-GaN layer were grown by metal organic chemical vapor deposition and an n++-GaN layer was deposited by molecular beam epitaxy to form a TJ. The TJ LEDs were then compared with equivalent LEDs having a tin-doped indium oxide (ITO) contact. The TJ LEDs exhibited a higher performance and a lower efficiency droop than did the ITO LEDs. At 35 A/cm2, the external quantum efficiencies for the TJ and ITO LEDs were 31.2 and 27%, respectively.

  18. Micro-light-emitting diodes with III–nitride tunnel junction contacts grown by metalorganic chemical vapor deposition

    KAUST Repository

    Hwang, David

    2017-12-13

    Micro-light-emitting diodes (µLEDs) with tunnel junction (TJ) contacts were grown entirely by metalorganic chemical vapor deposition. A LED structure was grown, treated with UV ozone and hydrofluoric acid, and reloaded into the reactor for TJ regrowth. The silicon doping level of the n++-GaN TJ was varied to examine its effect on voltage. µLEDs from 2.5 × 10−5 to 0.01 mm2 in area were processed, and the voltage penalty of the TJ for the smallest µLED at 20 A/cm2 was 0.60 V relative to that for a standard LED with indium tin oxide. The peak external quantum efficiency of the TJ LED was 34%.

  19. Micro-light-emitting diodes with III–nitride tunnel junction contacts grown by metalorganic chemical vapor deposition

    KAUST Repository

    Hwang, David; Mughal, Asad J.; Wong, Matthew S.; Alhassan, Abdullah I.; Nakamura, Shuji; DenBaars, Steven P.

    2017-01-01

    Micro-light-emitting diodes (µLEDs) with tunnel junction (TJ) contacts were grown entirely by metalorganic chemical vapor deposition. A LED structure was grown, treated with UV ozone and hydrofluoric acid, and reloaded into the reactor for TJ regrowth. The silicon doping level of the n++-GaN TJ was varied to examine its effect on voltage. µLEDs from 2.5 × 10−5 to 0.01 mm2 in area were processed, and the voltage penalty of the TJ for the smallest µLED at 20 A/cm2 was 0.60 V relative to that for a standard LED with indium tin oxide. The peak external quantum efficiency of the TJ LED was 34%.

  20. Regional differences in endothelial cell cytoskeleton, junctional proteins and phosphorylated tyrosine labeling in the porcine vortex vein system.

    Science.gov (United States)

    Tan, Priscilla Ern Zhi; Yu, Paula K; Yang, Hongfang; Cringle, Stephen J; Yu, Dao-Yi

    2018-07-01

    We previously demonstrated endothelial phenotype heterogeneity in the vortex vein system. This study is to further determine whether regional differences are present in the cytoskeleton, junctional proteins and phosphorylated tyrosine labeling within the system. The vortex vein system of twenty porcine eyes was perfused with labels for f-actin, claudin-5, VE-Cadherin, phosphorylated tyrosine and nucleic acid. The endothelial cells of eight different regions (choroidal veins, pre-ampulla, anterior ampulla, mid-ampulla, posterior ampulla, post-ampulla, intra-scleral canal and the extra-ocular vortex vein) were studied using confocal microscopy. There were regional differences in the endothelial cell structures. Cytoskeleton labeling was relatively even in intensity throughout Regions 1 to 6. Overall VE-Cadherin had a non-uniform distribution and thicker width endothelial cell border staining than claudin-5. Progressing downstream there was an increased variation in thickness of VE-cadherin labeling. There was an overlap in phosphorylated tyrosine and VE-Cadherin labeling in the post-ampulla, intra-scleral canal and extra-ocular vortex vein. Intramural cells were observed that were immune-positive for VE-Cadherin and phosphorylated tyrosine. There were significant differences in the number of intramural cells in different regions. Significant regional differences with endothelial cell labeling of cytoskeleton, junction proteins, and phosphorylated tyrosine were found within the vortex vein system. These findings support existing data on endothelial cell phenotype heterogeneity, and may aid in the knowledge of venous pathologies by understanding regions of vulnerability to endothelial damage within the vortex vein system. It could be valuable to further investigate and characterize the VE-cadherin and phosphotyrosine immune-positive intramural cells. Copyright © 2018. Published by Elsevier Ltd.

  1. Splenectomy after partial hepatectomy accelerates liver regeneration in mice by promoting tight junction formation via polarity protein Par 3-aPKC.

    Science.gov (United States)

    Liu, Guoxing; Xie, Chengzhi; Fang, Yu; Qian, Ke; Liu, Qiang; Liu, Gao; Cao, Zhenyu; Du, Huihui; Fu, Jie; Xu, Xundi

    2018-01-01

    Several experimental studies have demonstrated that removal of the spleen accelerates liver regeneration after partial hepatectomy. While the mechanism of splenectomy promotes liver regeneration by the improvement of the formation of tight junction and the establishment of hepatocyte polarity is still unknown. We analyzed the cytokines, genes and proteins expression between 70% partial hepatectomy mice (PHx) and simultaneous 70% partial hepatectomy and splenectomy mice (PHs) at predetermined timed points. Compared with the PHx group mice, splenectomy accelerated hepatocyte proliferation in PHs group. The expression of Zonula occludens-1 (ZO-1) indicated that splenectomy promotes the formation of tight junction during liver regeneration. TNF-α, IL-6, HGF, TSP-1 and TGF-β1 were essential factors for the formation of tight junction and the establishment of hepatocytes polarity in liver regeneration. After splenectomy, Partitioning defective 3 homolog (Par 3) and atypical protein kinase C (aPKC) regulate hepatocyte localization and junctional structures in regeneration liver. Our data suggest that the time course expression of TNF-α, IL-6, HGF, TSP-1, and TGF-β1 and the change of platelets take part in liver regeneration. Combination with splenectomy accelerates liver regeneration by improvement of the tight junction formation which may help to establish hepatocyte polarity via Par 3-aPKC. This may provide a clue for us that splenectomy could accelerate liver regeneration after partial hepatectomy of hepatocellular carcinoma and living donor liver transplantation. Copyright © 2017 Elsevier Inc. All rights reserved.

  2. ICRH studies in TJ-IU torsatron

    International Nuclear Information System (INIS)

    Castejon, F.; Longinov, A.V.; Rodriguez R, L.

    1993-01-01

    Preliminary studies for Ion Cyclotron Resonance Heating (ICRH) in the frequency range f=3-150 MHz are presented for TJ-IU torsatron. This wide range implies the use of two different theoretical models. The first valid for high frequency, where the WKB approximation is applicable, and the second one which solves the full wave equation in one dimension. The high frequency calculations have been made using a ray tracing code and taking into account the magnetic field and plasma 3-D inhomogeneity. The results obtained in this case are presented in the first paper of this report, being the most important the criterion to avoid Fast Wave (fw)-slow wave (SW) coupling at Lower Hybrid Resonance, near the plasma edge, and the existence of so called Localized Modes. for the low frequency range wave-length is of the size of the plasma radius, therefore, the WKB approximation cannot be used. In this case a 1-D model is used which disregards toroidal effects, to study the main available heating scenarios which are presented in the second work of this report. the studies are made for hydrogen, deuterium and mixed plasmas with and without He 3 minority. Finally, the antenna designs to reach these several scenarios are presented in the third paper. Two different antenna models are provided for SW excitation, one of the current type and the other one of potential type. A third antenna is designed to excite FW which is similar to the current type antenna for SW, but rotated 90 degree Celsius

  3. ICRH studies in TJ-IU torsatron

    International Nuclear Information System (INIS)

    Castejon, F.

    1993-01-01

    Preliminary studies for ion Cyclotron Resonance Heating (ICRH) in the frequency range f=3-150 MHz are presented for TJ-IU torsatron. This wide range implies the use of two different theoretical models. The first valid for high frequency, where the WKB approximation is applicable, and the second one which solves the full wave equation in one dimension. The high frequency calculations have been made using a ray tracing code and taking into account the magnetic field and plasma 3-D inhomogeneity. The results obtained in this case are presented in the first paper of this report, being the most important the criterion to avoid Fast Wave (FW)-Slow Wave (SW) coupling at Lower Hybrid Resonance, near the plasma edge, and the existence of so called Localized Modes. For the low frequency range wave-length is of the size of the plasma radius, there fore, the WKB approximation cannot be used. In this case a 1-D model is used which disregards toroidal effects, to study the main available heating scenarios which are presented in the second work of this report. The studies are made for hydrogen, deuterium and mixed plasmas with and without He3 majority. Finally, the antenna designs to reach these several scenarios are presented in the third paper. Two different antenna models are provided for SW excitation, one of the current type and the other one of potential type. A third antenna is designed to excite FW which is similar to the current type antenna for SW, but rotated 90 degree centigree. (Author)11 refs

  4. Nonmechanical Roles of Dystrophin and Associated Proteins in Exercise, Neuromuscular Junctions, and Brains

    Directory of Open Access Journals (Sweden)

    Bailey Nichols

    2015-07-01

    Full Text Available Dystrophin-glycoprotein complex (DGC is an important structural unit in skeletal muscle that connects the cytoskeleton (f-actin of a muscle fiber to the extracellular matrix (ECM. Several muscular dystrophies, such as Duchenne muscular dystrophy, Becker muscular dystrophy, congenital muscular dystrophies (dystroglycanopathies, and limb-girdle muscular dystrophies (sarcoglycanopathies, are caused by mutations in the different DGC components. Although many early studies indicated DGC plays a crucial mechanical role in maintaining the structural integrity of skeletal muscle, recent studies identified novel roles of DGC. Beyond a mechanical role, these DGC members play important signaling roles and act as a scaffold for various signaling pathways. For example, neuronal nitric oxide synthase (nNOS, which is localized at the muscle membrane by DGC members (dystrophin and syntrophins, plays an important role in the regulation of the blood flow during exercise. DGC also plays important roles at the neuromuscular junction (NMJ and in the brain. In this review, we will focus on recently identified roles of DGC particularly in exercise and the brain.

  5. Specific deletion of AMP-activated protein kinase (α1AMPK in murine oocytes alters junctional protein expression and mitochondrial physiology.

    Directory of Open Access Journals (Sweden)

    Michael J Bertoldo

    Full Text Available Oogenesis and folliculogenesis are dynamic processes that are regulated by endocrine, paracrine and autocrine signals. These signals are exchanged between the oocyte and the somatic cells of the follicle. Here we analyzed the role of AMP-activated protein kinase (AMPK, an important regulator of cellular energy homeostasis, by using transgenic mice deficient in α1AMPK specifically in the oocyte. We found a decrease of 27% in litter size was observed in ZP3-α1AMPK-/- (ZP3-KO female mice. Following in vitro fertilization, where conditions are stressful for the oocyte and embryo, ZP3-KO oocytes were 68% less likely to pass the 2-cell stage. In vivo and in cumulus-oocyte complexes, several proteins involved in junctional communication, such as connexin37 and N-cadherin were down-regulated in the absence of α1AMPK. While the two signalling pathways (PKA and MAPK involved in the junctional communication between the cumulus/granulosa cells and the oocyte were stimulated in control oocytes, ZP3-KO oocytes exhibited only low phosphorylation of MAPK or CREB proteins. In addition, MII oocytes deficient in α1AMPK had a 3-fold lower ATP concentration, an increase in abnormal mitochondria, and a decrease in cytochrome C and PGC1α levels, suggesting perturbed energy production by mitochondria. The absence of α1AMPK also induced a reduction in histone deacetylase activity, which was associated with an increase in histone H3 acetylation (K9/K14 residues. Together, the results of the present study suggest that absence of AMPK, modifies oocyte quality through energy processes and oocyte/somatic cell communication. The limited effect observed in vivo could be partly due to a favourable follicle microenvironment where nutrients, growth factors, and adequate cell interaction were present. Whereas in a challenging environment such as that of in vitro culture following IVF, the phenotype is revealed.

  6. Intraluminal polyethylene glycol stabilizes tight junctions and improves intestinal preservation in the rat.

    Science.gov (United States)

    Oltean, M; Joshi, M; Björkman, E; Oltean, S; Casselbrant, A; Herlenius, G; Olausson, M

    2012-08-01

    Rapidly progressing mucosal breakdown limits the intestinal preservation time below 10 h. Recent studies indicate that intraluminal solutions containing polyethylene glycol (PEG) alleviate preservation injury of intestines stored in UW-Viaspan. We investigated whether a low-sodium PEG solution is beneficial for intestines stored in histidine-tryptophane-ketoglutarate (HTK) preservation solution. Rat intestines used as control tissue (group 1) were perfused with HTK, groups 2 and 3 received either a customized PEG-3350 (group 2) or an electrolyte solution (group 3) intraluminally before cold storage. Tissue injury, brush-border maltase activity, zonula occludens-1 (ZO-1) and claudin-3 expression in the tight junctions (TJ) were analyzed after 8, 14 and 20 h. We measured epithelial resistance and permeability (Ussing chamber) after 8 and 14 h. Group 2 had superior morphology while maltase activity was similar in all groups. TJ proteins rapidly decreased and decolocalized in groups 1 3; these negative events were delayed in group 2, where colocalization persisted for about 14 h. Intestines in group 2 had higher epithelial resistance and lower permeability than the other groups. These results suggest that a customized PEG solution intraluminally reduces the intestinal preservation injury by improving several major epithelial characteristics without negatively affecting the brush-border enzymes or promoting edema. © Copyright 2012 The American Society of Transplantation and the American Society of Transplant Surgeons.

  7. Tight junctions at the blood brain barrier: physiological architecture and disease-associated dysregulation

    Directory of Open Access Journals (Sweden)

    Luissint Anny-Claude

    2012-11-01

    Full Text Available Abstract The Blood–brain barrier (BBB, present at the level of the endothelium of cerebral blood vessels, selectively restricts the blood-to-brain paracellular diffusion of compounds; it is mandatory for cerebral homeostasis and proper neuronal function. The barrier properties of these specialized endothelial cells notably depend on tight junctions (TJs between adjacent cells: TJs are dynamic structures consisting of a number of transmembrane and membrane-associated cytoplasmic proteins, which are assembled in a multimolecular complex and acting as a platform for intracellular signaling. Although the structural composition of these complexes has been well described in the recent years, our knowledge about their functional regulation still remains fragmentary. Importantly, pericytes, embedded in the vascular basement membrane, and perivascular microglial cells, astrocytes and neurons contribute to the regulation of endothelial TJs and BBB function, altogether constituting the so-called neurovascular unit. The present review summarizes our current understanding of the structure and functional regulation of endothelial TJs at the BBB. Accumulating evidence points to a correlation between BBB dysfunction, alteration of TJ complexes and progression of a variety of CNS diseases, such as stroke, multiple sclerosis and brain tumors, as well as neurodegenerative diseases like Parkinson’s and Alzheimer’s diseases. Understanding how TJ integrity is controlled may thus help improve drug delivery across the BBB and the design of therapeutic strategies for neurological disorders.

  8. Molecular and cellular mechanisms of tight junction dysfunction in the irritable bowel syndrome.

    Science.gov (United States)

    Cheng, Peng; Yao, Jianning; Wang, Chunfeng; Zhang, Lianfeng; Kong, Wuming

    2015-09-01

    The pathophysiological mechanisms of the irritable bowel syndrome (IBS), one of the most prevalent gastrointestinal disorders, are complex and have not been fully elucidated. The present study aimed to investigate the molecular and cellular mechanisms of tight junction (TJ) dysfunction in IBS. Intestinal tissues of IBS and non‑IBS patients were examined to observe cellular changes by cell chemical tracer electron microscopy and transmission electron microscopy, and intestinal claudin‑1 protein was detected by immunohistochemistry, western blot analysis and fluorescence quantitative polymerase chain reaction. Compared with the control group, TJ broadening and the tracer extravasation phenomenon were observed in the diarrhea‑predominant IBS group, and a greater number of neuroendocrine cells and mast cells filled with high‑density particles in the endocrine package pulp as well as a certain extent of vacuolization were present. The expression of claudin‑1 in diarrhea‑predominant IBS patients was decreased, while it was increased in constipation‑predominant IBS patients. In conclusion, the results of the present study indicated that changes in cellular structure and claudin‑1 levels were associated with Tjs in IBS.

  9. Overview of the TJ-II remote participation system

    International Nuclear Information System (INIS)

    Vega, J.; Sanchez, E.; Portas, A.; Pereira, A.; Mollinedo, A.; Munoz, J.A.; Ruiz, M.; Barrera, E.; Lopez, S.; Machon, D.; Castro, R.; Lopez, D.

    2006-01-01

    The TJ-II remote participation system (RPS) is focused on providing remote access to elements that depend exclusively on characteristics of the TJ-II environment: data acquisition, diagnostics control systems and TJ-II operation tracking. Four key points were taken into account prior to starting the software design: access security, software execution platforms, software maintenance and distribution and delivery of operation events. The first, access security, was addressed by means of a distributed authentication and authorization system, PAPI. Regarding the other points, the development was based on the use of web servers (due to their standard character, flexibility and scalability) and Java technologies (due to their open nature, security properties and technological maturity). Software deployment was prepared to make use of the Java Network Launching Protocol (JNLP). On-line message distribution was planned according to a message oriented middleware. At present, the TJ-II RPS manages over 1000 digitization channels and 20 diagnostic control systems. The TJ-II RPS architecture is flexible, scalable and powerful enough to be applied to distributed environments and, in particular, it could be used in the ITER environment

  10. Overview of the TJ-II remote participation system

    Energy Technology Data Exchange (ETDEWEB)

    Vega, J. [Asociacion EURATOM/CIEMAT para Fusion, Avda. Complutense, 22, 28040 Madrid (Spain)]. E-mail: jesus.vega@ciemat.es; Sanchez, E. [Asociacion EURATOM/CIEMAT para Fusion, Avda. Complutense, 22, 28040 Madrid (Spain); Portas, A. [Asociacion EURATOM/CIEMAT para Fusion, Avda. Complutense, 22, 28040 Madrid (Spain); Pereira, A. [Asociacion EURATOM/CIEMAT para Fusion, Avda. Complutense, 22, 28040 Madrid (Spain); Mollinedo, A. [Computer Centre, CIEMAT, Avda. Complutense, 22, 28040 Madrid (Spain); Munoz, J.A. [Computer Centre, CIEMAT, Avda. Complutense, 22, 28040 Madrid (Spain); Ruiz, M. [Dpto. De Sistemas Electronicos y de Control, UPM, Campus Sur, Ctra. Valencia km 7, 28031 Madrid (Spain); Barrera, E. [Dpto. De Sistemas Electronicos y de Control, UPM, Campus Sur, Ctra. Valencia km 7, 28031 Madrid (Spain); Lopez, S. [Dpto. De Sistemas Electronicos y de Control, UPM, Campus Sur, Ctra. Valencia km 7, 28031 Madrid (Spain); Machon, D. [Dpto. De Sistemas Electronicos y de Control, UPM, Campus Sur, Ctra. Valencia km 7, 28031 Madrid (Spain); Castro, R. [Red.es-RedIRIS, Edificio Bronce, Plaza Manuel Gomez Moreno s/n, 28020 Madrid (Spain); Lopez, D. [Red.es-RedIRIS, Edificio Bronce, Plaza Manuel Gomez Moreno s/n, 28020 Madrid (Spain)

    2006-07-15

    The TJ-II remote participation system (RPS) is focused on providing remote access to elements that depend exclusively on characteristics of the TJ-II environment: data acquisition, diagnostics control systems and TJ-II operation tracking. Four key points were taken into account prior to starting the software design: access security, software execution platforms, software maintenance and distribution and delivery of operation events. The first, access security, was addressed by means of a distributed authentication and authorization system, PAPI. Regarding the other points, the development was based on the use of web servers (due to their standard character, flexibility and scalability) and Java technologies (due to their open nature, security properties and technological maturity). Software deployment was prepared to make use of the Java Network Launching Protocol (JNLP). On-line message distribution was planned according to a message oriented middleware. At present, the TJ-II RPS manages over 1000 digitization channels and 20 diagnostic control systems. The TJ-II RPS architecture is flexible, scalable and powerful enough to be applied to distributed environments and, in particular, it could be used in the ITER environment.

  11. Prophylactic effect of rebamipide on aspirin-induced gastric lesions and disruption of tight junctional protein zonula occludens-1 distribution.

    Science.gov (United States)

    Suzuki, Takahiro; Yoshida, Norimasa; Nakabe, Nami; Isozaki, Yutaka; Kajikawa, Hirokazu; Takagi, Tomohisa; Handa, Osamu; Kokura, Satoshi; Ichikawa, Hiroshi; Naito, Yuji; Matsui, Hirofumi; Yoshikawa, Toshikazu

    2008-03-01

    Aspirin and nonsteroidal anti-inflammatory agents are known to induce gastroduodenal complications such as ulcer, bleeding, and dyspepsia. In this study, we examined the prophylactic effect of rebamipide, an anti-ulcer agent with free-radical scavenging and anti-inflammatory effect, on acidified aspirin-induced gastric mucosal injury in rats. In addition, we investigated the mucosal barrier functions disrupted by aspirin. Oral administration of acidified aspirin resulted in linear hemorrhagic erosions with increasing myeloperoxidase activity and thiobarbituric acid-reactive substance concentrations in the gastric mucosa. Rebamipide suppressed these acidified aspirin-induced gastric lesions and inflammatory changes significantly, and its protective effect was more potent in the case of repeated (twice daily for 3 days) treatment than single treatment before aspirin administration. Immunostaining of zonula occludens (ZO)-1, one of the tight junctional proteins, was strengthened in rat gastric mucosa after repeated administration of rebamipide. In addition, aspirin induced the increasing transport of fluorescine isothiocyanate-labeled dextrans with localized disruption and decreased expression of ZO-1 protein on rat gastric mucosal cell line RGM-1. Rebamipide effectively prevented aspirin-induced permeability changes and disruption of ZO-1 distribution. These results suggest that rebamipide protects against aspirin-induced gastric mucosal lesions by preserving gastric epithelial cell-to cell integrity in addition to the anti-inflammatory effects.

  12. Resolution of Holliday junctions in Escherichia coli: identification of the ruvC gene product as a 19-kilodalton protein.

    Science.gov (United States)

    Sharples, G J; Lloyd, R G

    1991-12-01

    The ruvC gene of Escherichia coli specifies a nuclease that resolves Holliday junction intermediates in genetic recombination (B. Connolly, C.A. Parsons, F.E. Benson, H.J. Dunderdale, G.J. Sharples, R.G. Lloyd, and S.C. West, Proc. Natl. Acad, Sci. USA 88:6063-6067, 1991). The gene was located between aspS and the ruvAB operon by DNA sequencing and deletion analysis of ruvC plasmids and was shown to encode a protein of 18,747 Da. Analysis of the DNA flanking ruvC indicated that the gene is transcribed independently of the LexA-regulated ruvAB operon and is not under direct SOS control. ruvC lies downstream of an open reading frame, orf-33, for a protein which migrates during sodium dodecyl sulfate-polyacrylamide gel electrophoresis as a 33-kDa polypeptide. These two genes probably form an operon. However, expression of ruvC was found to be very poor relative to that of orf-33. A double ribosomal frameshift between these genes is proposed as a possible reason for the low level of RuvC. Two further open reading frames of unknown function were identified, one on either side of the orf-33-ruvC operon.

  13. Remote Control of TJ-II Diagnostics; Control Remoto de Diagnosticos del Dispositivo TJ-II

    Energy Technology Data Exchange (ETDEWEB)

    Lopez Sanchez, A.; Vega, J.; Montoro, A.; Encabo, J.

    2001-07-01

    The present paper is about the design and development of ten remote control diagnostic systems used in the study of plasma fusion in the TJ-II device installed at CIEMAT. This development goes from the definition of sensors and devices necessary in carrying out these remote controls, to its assembly, wiring, development of electronic circuits inserted between sensors and PLC, development of programs for these PLC, connections and administration of the real time automation network, and later development of the necessary programs via the appropriate software tools for web access through a navigator to a specific web page, allowing visual and real time access over the auxiliary systems that make up all the diagnostics. (Author)

  14. Solar cell junction temperature measurement of PV module

    KAUST Repository

    Huang, B.J.

    2011-02-01

    The present study develops a simple non-destructive method to measure the solar cell junction temperature of PV module. The PV module was put in the environmental chamber with precise temperature control to keep the solar PV module as well as the cell junction in thermal equilibrium with the chamber. The open-circuit voltage of PV module Voc is then measured using a short pulse of solar irradiation provided by a solar simulator. Repeating the measurements at different environment temperature (40-80°C) and solar irradiation S (200-1000W/m2), the correlation between the open-circuit voltage Voc, the junction temperature Tj, and solar irradiation S is derived.The fundamental correlation of the PV module is utilized for on-site monitoring of solar cell junction temperature using the measured Voc and S at a short time instant with open circuit. The junction temperature Tj is then determined using the measured S and Voc through the fundamental correlation. The outdoor test results show that the junction temperature measured using the present method, Tjo, is more accurate. The maximum error using the average surface temperature Tave as the junction temperature is 4.8 °C underestimation; while the maximum error using the present method is 1.3 °C underestimation. © 2010 Elsevier Ltd.

  15. Application for TJ-II Signals Visualization: User's Guide

    International Nuclear Information System (INIS)

    Sanchez, E.; Portas, A. B.; Cremy, C.; Vega, J.

    2000-01-01

    In this documents are described the functionalities of the application developed by the Data Acquisition Group for TJ-II signal visualization. There are two versions of the application, the On-line version, used for signal visualization during TJ-II operation, and the Off-line version, used for signal visualization without TJ-II operation. Both versions of the application consist in a graphical user interface developed for X/Motif, in which most of the actions can be done using the mouse buttons. The functionalities of both versions of the application are described in this user's guide, beginning at the application start-up and explaining in detail all the options that it provides and the actions that can be done with each graphic control. (Author) 8 refs

  16. Confinement studies in the TJ-II stellarator

    International Nuclear Information System (INIS)

    Alejaldre, C.; Alonso, J.; Almoguera, L.; Ascasibar, E.; Baciero, A.; Balbin, R.; Blaumoser, M.; Botija, J.; Branas, B.; Cal, E. de la; Cappa, A.; Carrasco, R.; Castejon, F.; Cepero, J.R.; Cremy, C.; Delgado, J.M.; Doncel, J.; Dulya, C.; Estrada, T.; Fernandez, A.; Fuentes, C.; Garcia, A.; Garcia-Cortes, I.; Guasp, J.; Herranz, J.; Hidalgo, C.; Jimenez, J.A.; Kirpitchev, I.; Krivenski, V.; Labrador, I.; Lapayese, F.; Likin, K.; Linier, M.; Lopez-Fraguas, A.; Lopez-Sanchez, A.; Luna, E. de la; Martin, R.; Martinez, A.; Martinez-Laso, L.; Medrano, M.; Mendez, P.; McCarthy, K.J.; Medina, F.; Milligen, B. van; Ochando, M.; Pacios, L.; Pastor, I.; Pedrosa, M.A.; Pena, A. de la; Portas, A.; Qin, J.; Rodriguez-Rodrigo, L.; Salas, A.; Sanchez, E.; Sanchez, J.; Tabares, F.; Tafalla, D.; Tribaldos, V.; Vega, J.; Zurro, B.; Akulina, D.; Fedyanin, O.I.; Grebenshchikov, S.; Kharchev, N.; Meshcheryakov, A.; Sarksian, K.A.; Barth, R.; Dijk, G. van; Meiden, H. van der

    1999-01-01

    ECR (electron cyclotron resonance) heated plasmas have been studied in the low magnetic shear TJ-II stellarator (R = 1.5 m, a ECRH = 300 kW, power density = 1-25 W cm -3 ). Recent experiments have explored the flexibility of the TJ-II across a wide range of plasma volumes with different rotational transforms and rational surface densities. In this paper, the main results of this campaign are presented and, in particular, the influence of iota and rational surfaces on plasma profiles is discussed. (author)

  17. First plasmas in the TJ-II flexible Heliac

    International Nuclear Information System (INIS)

    Alejaldre, C.; Alonso, J.; Almoguera, L.; Ascasibar, E.; Baciero, A.; Balbin, R.; Blaumoser, M.; Botija, J.; Branas, B.; Cal, E. de la; Cappa, A.; Carrasco, R.; Castejon, F.; Cepero, J.R.; Cremy, C.; Doncel, J.; Dulya, C.; Estrada, T.; Fernandez, A.; Frances, M.; Fuentes, C.; Garcia, A.; Garcia-Cortes, I.; Guasp, J.; Herranz, J.; Hidalgo, C.; Jimenez, J.A.; Kirpitchev, I.; Krivenski, V.; Labrador, I.; Lapayese, F.; Likin, K.; Liniers, M.; Lopez-Fraguas, A.; Lopez-Sanchez, A.; Luna, E. de la; Martin, R.; Martinez, A.; Medrano, M.; Mendez, P.; McCarthy, K.; Medina, F.; Milligen, B. van; Ochando, M.; Pacios, L.; Pastor, I.; Pedrosa, M.A.; Pena, A. de la; Portas, A.; Qin, J.; Rodriguez-Rodrigo, L.; Salas, A.; Sanchez, E.; Sanchez, J.; Tabares, F.; Tafalla, D.; Tribaldos, V.; Vega, J.; Zurro, B.; Akulina, D.; Fedyanin, O.I.; Grebenshchicov, S.; Kharchev, N.; Meshcheryakov, A.; Barth, R.; Dijk, G. van; Meiden, H. van der; Petrov, S.

    1999-01-01

    The first experimental campaign of the TJ-II stellarator has been conducted using electron cyclotron resonance heating (f=53.2 GHz, P ECRH approx. 250 kW) with a pulse length of Δt approx. (80-200) ms. The flexibility of the device has been used to study five different configurations varying plasma volume and rotational transform. In this paper, the main results of this campaign are presented and, in particular, the influence of plasma-wall interaction phenomena on TJ-II confinement is briefly discussed. (author)

  18. SENP3 grants tight junction integrity and cytoskeleton architecture in mouse Sertoli cells.

    Science.gov (United States)

    Wu, Di; Huang, Chun-Jie; Khan, Faheem Ahmed; Jiao, Xiao-Fei; Liu, Xiao-Ming; Pandupuspitasari, Nuruliarizki Shinta; Brohi, Rahim Dad; Huo, Li-Jun

    2017-08-29

    Germ cells develop in a sophisticated immune privileged microenvironment provided by specialized junctions contiguous the basement membrane of the adjacent Sertoli cells that constituted the blood-testis barrier (BTB) in seminiferous epithelium of testis in mammals. Deciphering the molecular regulatory machinery of BTB activity is central to improve male fertility and the role of post-translational modification including SUMOylation pathway is one of the key factors. Herein, we unveiled the mystery of the SUMO-2/3 specific protease SENP3 (Sentrin-specific protease 3) in BTB dynamics regulation. SENP3 is predominantly expressed in the nucleus of Sertoli and spermatocyte cells in adult mouse testis, and knockdown of SENP3 compromises tight junction in Sertoli cells by destructing the permeability function with a concomitant decline in trans-epithelial electrical resistance in primary Sertoli cells, which could attribute to the conspicuous dysfunction of tight junction (TJ) proteins (e.g., ZO-1, occludin) at the cell-cell interface due to the inactivation of STAT3. Moreover, SENP3 knockdown disrupts F-actin architecture in Sertoli cells through intervening Rac1/CDC42-N-WASP-Arp2/3 signaling pathway and Profilin-1 abundance. Our study pinpoints SENP3 might be a novel determinant of multiple pathways governing BTB dynamics in testis to support germ cells development in mammals.

  19. Cell polarity development and protein trafficking in hepatocytes lacking E-cadherin/beta-catenin-based adherens junctions

    NARCIS (Netherlands)

    Theard, Delphine; Steiner, Magdalena; Kalicharan, Dharamdajal; Hoekstra, Dick; van IJzendoorn, Sven C. D.

    Using a mutant hepatocyte cell line in which E-cadherin and ss-catenin are completely depleted from the cell surface, and, consequently, fail to form adherens junctions, we have investigated adherens junction requirement for apical-basolateral polarity development and polarized membrane trafficking.

  20. MOSFET-BJT hybrid mode of the gated lateral bipolar junction transistor for C-reactive protein detection.

    Science.gov (United States)

    Yuan, Heng; Kwon, Hyurk-Choon; Yeom, Se-Hyuk; Kwon, Dae-Hyuk; Kang, Shin-Won

    2011-10-15

    In this study, we propose a novel biosensor based on a gated lateral bipolar junction transistor (BJT) for biomaterial detection. The gated lateral BJT can function as both a BJT and a metal-oxide-semiconductor field-effect transistor (MOSFET) with both the emitter and source, and the collector and drain, coupled. C-reactive protein (CRP), which is an important disease marker in clinical examinations, can be detected using the proposed device. In the MOSFET-BJT hybrid mode, the sensitivity, selectivity, and reproducibility of the gated lateral BJT for biosensors were evaluated in this study. According to the results, in the MOSFET-BJT hybrid mode, the gated lateral BJT shows good selectivity and reproducibility. Changes in the emitter (source) current of the device for CRP antigen detection were approximately 0.65, 0.72, and 0.80 μA/decade at base currents of -50, -30, and -10 μA, respectively. The proposed device has significant application in the detection of certain biomaterials that require a dilution process using a common biosensor, such as a MOSFET-based biosensor. Copyright © 2011 Elsevier B.V. All rights reserved.

  1. Changes of Tight Junction Protein Claudins in Small Intestine and Kidney Tissues of Mice Fed a DDC Diet.

    Science.gov (United States)

    Abiko, Yukie; Kojima, Takashi; Murata, Masaki; Tsujiwaki, Mitsuhiro; Takeuchi, Masaya; Sawada, Norimasa; Mori, Michio

    2013-12-01

    DDC (3,5-diethoxycarbonyl-1,4-dihydrocollidine)-fed mice are widely used as a model for cholestatic liver disease. We examined the expression of tight junction protein claudin subspecies by immunofluorescent histochemistry in small intestine and kidney tissues of mice fed a DDC diet for 12 weeks. In the small intestine, decreases in claudin-3, claudin-7 and claudin-15 were observed in villous epithelial cells corresponding to the severity of histological changes while leaving the abundance of these claudin subspecies unchanged in crypt cells. Nevertheless, the proliferative activity of intestinal crypt cells measured by immunohistochemistry for Ki-67 decreased in the mice fed the DDC diet compared with that of control mice. These results suggest the possibility that DDC feeding affects the barrier function of villous epithelial cells and thus inhibits the proliferative activity of crypt epithelial cells. On the other hand, in the kidney, remarkable changes were found in the subcellular localization of claudin subspecies in a segment-specific manner, although histological changes of renal epithelial cells were quite minimal. These results indicate that immunohistochemistry for claudin subspecies can serve as a useful tool for detecting minute functional alterations of intestinal and renal epithelial cells.

  2. Profile structures of TJ-II stellarator plasmas

    NARCIS (Netherlands)

    Herranz, J.; Pastor, I.; Castejon, F.; de la Luna, E.; Garcia-Cortes, I.; Barth, C. J.; Ascasibar, E.; Sanchez, J.; Tribaldos, V.

    2000-01-01

    Fine structures are found in the TJ-II stellarator electron temperature and density profiles, when they are measured using a high spatial resolution Thomson scattering system. These structures consist of peaks and valleys superimposed to a smooth average. Some irregularities remain in an ensemble

  3. A synchronization system to digitalize TJ-1 Tokamak data

    International Nuclear Information System (INIS)

    Guasp, J.; Perez-Navarro, A.; Pacios, L.

    1983-01-01

    At TJ-1 Tokamak signals are stored on a 60-channel magnetic memory. In this report, a system to address those channels and synchronize readout is presented. Digitalized signals are stored in structured files on PDP-11/34 magnetic disks. (author)

  4. Measurements of plasma position in TJ-I Tokamak

    International Nuclear Information System (INIS)

    Qin, J.; Ascasibar, E.; Navarro, A.P.; Ochando, M.A.; Pastor, I.; Pedrosa, M.A.; Rodriguez, L.; Sanchez, J.; Team, TJ-I.

    1994-01-01

    This report presents the experimental measurements of plasma position in TJ-I tokamak by using small magnetic probes. The basis of method has been described in our previous work (1) in which the plasma current is considered as a filament current. The observed relations between the disruptive instabilities and plasma displacements are also show here. (Author) 7 refs

  5. Peripheral Codes in ASTRA for the TJ-II

    International Nuclear Information System (INIS)

    Lopez-Bruna, D.; Reynolds, J. M.; Cappa, A.; Martinell, J.; Garcia, J.; Gutierrez-Tapia, C.

    2010-01-01

    The study of data from the TJ-II device is often done with transport calculations based on the ASTRA transport system. However, complicated independent codes are used to obtain fundamental ingredients in these calculations, such as the particle and/or energy sources. These codes are accessible from ASTRA through the procedures explained in this report. (Author) 37 refs.

  6. First plasmas in the TJ-II flexible Heliac

    NARCIS (Netherlands)

    Alejaldre, C.; Alonso, J.; Almoguera, L.; Ascasibar, E.; Baciero, A.; Balbin, R.; Blaumoser, M.; Botija, J.; Branas, B.; De La Cal, E.; Cappa, A.; Carrasco, R.; Castejon, F.; Cepero, J. R.; Cremy, C.; Doncel, J.; Dulya, C.; Estrada, T.; Fernandez, A.; Frances, M.; Fuentes, C.; Garcia, A.; Garcia-Cortes, I.; Guasp, J.; Herranz, J.; Hidalgo, C.; Jimenez, J. A.; Kirpitchev, I.; Krivenski, V.; Labrador, I.; Lapayese, F.; Likin, K.; Liniers, M.; Lopez-Fraguas, A.; Lopez-Sanchez, A.; de la Luna, E.; Martin, R.; Martinez, A.; Medrano, M.; Mendez, P.; McCarthy, K.; Medina, F.; van Milligen, B.; Ochando, M.; Pacios, L.; Pastor, I.; Pedrosa, M. A.; de la Pena, A.; Portas, A.; Qin, J.; Rodriguez-Rodrigo, L.; Salas, A.; Sanchez, E.; Sanchez, J.; Tabares, F.; Tafalla, D.; Tribaldos, V.; Vaga, J.; Zurro, B.; Akulina, D.; Fadyanin, O. I.; Grebenshchicov, S.; Kharchev, N.; Meshcheryakov, A.; Barth, R.; van Dijk, G.; van der Meiden, H.; Petrov, S.

    1999-01-01

    The first experimental campaign of the TJ-II stellarator has been conducted using electron cyclotron resonance heating (f = 53.2 GHz, P-ECRH approximate to 250 kW) with a pulse length of Delta t approximate to (80-200) ms. The flexibility of the device has been used to study five different

  7. Peripheral Codes in ASTRA for the TJ-II; Programas Perifericos de ASTRA para el TJ-II

    Energy Technology Data Exchange (ETDEWEB)

    Lopez-Bruna, D.; Reynolds, J. M.; Cappa, A.; Martinell, J.; Garcia, J.; Gutierrez-Tapia, C.

    2010-05-01

    The study of data from the TJ-II device is often done with transport calculations based on the ASTRA transport system. However, complicated independent codes are used to obtain fundamental ingredients in these calculations, such as the particle and/or energy sources. These codes are accessible from ASTRA through the procedures explained in this report. (Author) 37 refs.

  8. Gonadotropin suppression in men leads to a reduction in claudin-11 at the Sertoli cell tight junction.

    Science.gov (United States)

    McCabe, M J; Tarulli, G A; Laven-Law, G; Matthiesson, K L; Meachem, S J; McLachlan, R I; Dinger, M E; Stanton, P G

    2016-04-01

    Are Sertoli cell tight junctions (TJs) disrupted in men undergoing hormonal contraception? Localization of the key Sertoli cell TJ protein, claudin-11, was markedly disrupted by 8 weeks of gonadotropin suppression, the degree of which was related to the extent of adluminal germ cell suppression. Sertoli cell TJs are vital components of the blood-testis barrier (BTB) that sequester developing adluminal meiotic germ cells and spermatids from the vascular compartment. Claudin-11 knockout mice are infertile; additionally claudin-11 is spatially disrupted in chronically gonadotropin-suppressed rats coincident with a loss of BTB function, and claudin-11 is disorganized in various human testicular disorders. These data support the Sertoli cell TJ as a potential site of hormonal contraceptive action. BTB proteins were assessed by immunohistochemistry (n = 16 samples) and mRNA (n = 18 samples) expression levels in available archived testis tissue from a previous study of 22 men who had undergone 8 weeks of gonadotropin suppression and for whom meiotic and post-meiotic germ cell numbers were available. The gonadotropin suppression regimens were (i) testosterone enanthate (TE) plus the GnRH antagonist, acyline (A); (ii) TE + the progestin, levonorgestrel, (LNG); (iii) TE + LNG + A or (iv) TE + LNG + the 5α-reductase inhibitor, dutasteride (D). A control group consisted of seven additional men, with three archived samples available for this study. Immunohistochemical localization of claudin-11 (TJ) and other junctional type markers [ZO-1 (cytoplasmic plaque), β-catenin (adherens junction), connexin-43 (gap junction), vinculin (ectoplasmic specialization) and β-actin (cytoskeleton)] and quantitative PCR was conducted using matched frozen testis tissue. Claudin-11 formed a continuous staining pattern at the BTB in control men. Regardless of gonadotropin suppression treatment, claudin-11 localization was markedly disrupted and was broadly associated with the extent of meiotic

  9. Role of gap junctions and protein kinase A during the development of oocyte maturational competence in Ayu (Plecoglossus altivelis)

    Science.gov (United States)

    Yamamoto, Y.; Yoshizaki, G.; Takeuchi, T.; Soyano, K.; Patino, R.

    2008-01-01

    Meiotic resumption in teleost oocytes is induced by a maturation-inducing hormone (MIH). The sensitivity of oocytes to MIH, also known as oocyte maturational competence (OMC), is induced by LH via mechanisms that are not fully understood. A previous study of Ayu (Plecoglossus altivelis) showed the presence of functional heterologous gap junctions (GJs) between oocytes and their surrounding granulosa cells. The objectives of this study were to determine the role of ovarian GJs and of protein kinase A (PKA) during the acquisition of OMC. We examined the effects of the specific GJ inhibitor carbenoxolone (CBX) and 18??-glycyrrhetinic acid (??-GA) on the LH-(hCG)-dependent acquisition of OMC and on MIH-(17,20??-dihydroxy-4-pregnen-3-one)-dependent meiotic resumption; measured the cAMP content of ovarian follicles during the hCG-dependent acquisition of OMC; and determined the effects of PK activators and inhibitors on hCG-dependent OMC. Production of follicular cAMP increased during the hCG-dependent acquisition of OMC. Both GJ inhibitors and the PKA inhibitor H8-dihydrochloride, but not the PKC inhibitor GF109203X, suppressed the hCG-dependent acquisition of OMC in a dose-dependent manner. The PKA activator forskolin induced OMC with a similar potency to hCG. Unlike previous observations with teleosts where disruption of heterologous GJ either blocks or stimulates meiotic resumption, treatment with GJ inhibitors did not affect MIH-dependent meiotic resumption in maturationally competent follicles of Ayu. These observations suggest that ovarian GJs are essential for LH-dependent acquisition of OMC but not for MIH-dependent meiotic resumption, and that the stimulation of OMC by LH is mediated by cAMP-dependent PKA. They are also consistent with the view that a precise balance between GJ-mediated signals (positive or negative) and oocyte maturational readiness is required for hormonally regulated meiotic resumption. ?? 2007 Elsevier Inc. All rights reserved.

  10. Gap Junctions

    Science.gov (United States)

    Nielsen, Morten Schak; Axelsen, Lene Nygaard; Sorgen, Paul L.; Verma, Vandana; Delmar, Mario; Holstein-Rathlou, Niels-Henrik

    2013-01-01

    Gap junctions are essential to the function of multicellular animals, which require a high degree of coordination between cells. In vertebrates, gap junctions comprise connexins and currently 21 connexins are known in humans. The functions of gap junctions are highly diverse and include exchange of metabolites and electrical signals between cells, as well as functions, which are apparently unrelated to intercellular communication. Given the diversity of gap junction physiology, regulation of gap junction activity is complex. The structure of the various connexins is known to some extent; and structural rearrangements and intramolecular interactions are important for regulation of channel function. Intercellular coupling is further regulated by the number and activity of channels present in gap junctional plaques. The number of connexins in cell-cell channels is regulated by controlling transcription, translation, trafficking, and degradation; and all of these processes are under strict control. Once in the membrane, channel activity is determined by the conductive properties of the connexin involved, which can be regulated by voltage and chemical gating, as well as a large number of posttranslational modifications. The aim of the present article is to review our current knowledge on the structure, regulation, function, and pharmacology of gap junctions. This will be supported by examples of how different connexins and their regulation act in concert to achieve appropriate physiological control, and how disturbances of connexin function can lead to disease. © 2012 American Physiological Society. Compr Physiol 2:1981-2035, 2012. PMID:23723031

  11. Gap junctions and motor behavior

    DEFF Research Database (Denmark)

    Kiehn, Ole; Tresch, Matthew C.

    2002-01-01

    The production of any motor behavior requires coordinated activity in motor neurons and premotor networks. In vertebrates, this coordination is often assumed to take place through chemical synapses. Here we review recent data suggesting that electrical gap-junction coupling plays an important role...... in coordinating and generating motor outputs in embryonic and early postnatal life. Considering the recent demonstration of a prevalent expression of gap-junction proteins and gap-junction structures in the adult mammalian spinal cord, we suggest that neuronal gap-junction coupling might also contribute...... to the production of motor behavior in adult mammals....

  12. Regulation of intercellular tight junctions by zonula occludens toxin and its eukaryotic analogue zonulin.

    Science.gov (United States)

    Fasano, A

    2000-01-01

    The intestinal epithelium represents the largest interface between the external environment and the internal host milieu and constitutes the major barrier through which molecules can either be absorbed or secreted. There is now substantial evidence that tight junctions (tj) play a major role in regulating epithelial permeability by influencing paracellular flow of fluid and solutes. Tj are one of the hallmarks of absorptive and secretory epithelia. Evidence now exists that tj are dynamic rather than static structures and readily adapt to a variety of developmental, physiological, and pathological circumstances. These adaptive mechanisms are still incompletely understood. Activation of PKC either by Zonula occludens toxin (Zot) or by phorbol esters increases paracellular permeability. Alteration of epithelial tj is a recently described property for infectious agents. Clostridium difficile toxin A and B and influenza and vesicular stomatitis viruses have been shown to loosen tj in tissue culture monolayers. Unlike what occurs after the Zot stimulus, these changes appear to be irreversible and are associated with destruction of the tj complex. On the basis of this observation, we postulated that Zot may mimic the effect of a functionally and immunologically related endogenous modulator of epithelial tj. We were able to identify an intestinal Zot analogue, which we named zonulin. It is conceivable that the zonulins participate in the physiological regulation of intercellular tj not only in the small intestine, but also throughout a wide range of extraintestinal epithelia as well as the ubiquitous vascular endothelium, including the blood-brain barrier. Disregulation of this hypothetical zonulin model may contribute to disease states that involve disordered intercellular communication, including developmental and intestinal disorders, tissue inflammation, malignant transformation, and metastasis.

  13. Generalized flux states of the t-J model

    International Nuclear Information System (INIS)

    Nori, F.; Abrahams, E.; Zimanyi, G.T.

    1990-01-01

    We investigate certain generalized flux phases arising in a mean-field approach to the t-J model. First, we establish that the energy of noninteracting electrons moving in a uniform magnetic field has an absolute minimum as a function of the flux at exactly one flux quantum per particle. Using this result, we show that if the hard-core nature of the hole bosons is taken into account, then the slave-boson mean-field approximation for the t-J Hamiltonian allows for a solution where both the spinons and the holons experience an average flux of one flux quantum per particle. This enables them to achieve the lowest possible energy within the manifold of spatially uniform flux states. In the case of the continuum model, this is possible only for certain fractional fillings and we speculate that the system may react to this frustration effect by phase separation

  14. Neutral beam injection optimization at TJ-II

    International Nuclear Information System (INIS)

    Fuentes, C.; Liniers, M.; Wolfers, G.; Alonso, J.; Marcon, G.; Carrasco, R.; Guasp, J.; Acedo, M.; Sanchez, E.; Medrano, M.; Garcia, A.; Doncel, J.; Alejaldre, C.; Tsai, C.C.; Barber, G.; Sparks, D.

    2005-01-01

    Neutral beam injection (NBI) heating has been used on the TJ-II stellarator for the first time. The beam has a port-through power between 200 and 400 kW and injection energy 28 kV. Beam transmission is limited by beam interception at the injection port and the first toroidal field coil, therefore, beam steering optimization is of critical importance. The beam interaction areas inside TJ-II vacuum chamber are surveyed by infrared thermography. Beam reionization can be a problem due to the presence of residual gas in the duct region. Halpha emission is used to monitor the reionization at the duct. A careful optimization of the injected gas has been carried out

  15. Interaction of Ddc1 and RPA with single-stranded/double-stranded DNA junctions in yeast whole cell extracts: Proteolytic degradation of the large subunit of replication protein A in ddc1Δ strains.

    Science.gov (United States)

    Sukhanova, Maria V; D'Herin, Claudine; Boiteux, Serge; Lavrik, Olga I

    2014-10-01

    To characterize proteins that interact with single-stranded/double-stranded (ss/ds) DNA junctions in whole cell free extracts of Saccharomyces cerevisiae, we used [(32)P]-labeled photoreactive partial DNA duplexes containing a 3'-ss/ds-junction (3'-junction) or a 5'-ss/ds-junction (5'-junction). Identification of labeled proteins was achieved by MALDI-TOF mass spectrometry peptide mass fingerprinting and genetic analysis. In wild-type extract, one of the components of the Ddc1-Rad17-Mec3 complex, Ddc1, was found to be preferentially photocrosslinked at a 3'-junction. On the other hand, RPAp70, the large subunit of the replication protein A (RPA), was the predominant crosslinking product at a 5'-junction. Interestingly, ddc1Δ extracts did not display photocrosslinking of RPAp70 at a 5'-junction. The results show that RPAp70 crosslinked to DNA with a 5'-junction is subject to limited proteolysis in ddc1Δ extracts, whereas it is stable in WT, rad17Δ, mec3Δ and mec1Δ extracts. The degradation of the RPAp70-DNA adduct in ddc1Δ extract is strongly reduced in the presence of the proteasome inhibitor MG 132. We also addressed the question of the stability of free RPA, using anti-RPA antibodies. The results show that RPAp70 is also subject to proteolysis without photocrosslinking to DNA upon incubation in ddc1Δ extract. The data point to a novel property of Ddc1, modulating the turnover of DNA binding proteins such as RPAp70 by the proteasome. Copyright © 2014 Elsevier B.V. All rights reserved.

  16. Control system for the Spanish Stellarator TJ-II

    International Nuclear Information System (INIS)

    Pacios, L.; Blaumoser, M.; Pena, A. de la; Carrasco, R.; Labrador, I.; Lapayese, F.; Diaz, J.C.; Laso, L.M.

    1995-01-01

    We describe the distributed control and monitoring system for the Spanish Stellarator TJ-II, which is under construction at CIEMAT in Madrid. It consists of one central UNIX workstation and several autonomous subsystems based on VME crates with embedded processors under OS-9 real-time operating system and PLCs. The system integrates the machine and discharge control. An operator can perform the control and plasma discharge by means of a user-friendly graphic interface. (orig.)

  17. Tight junctions and human diseases.

    Science.gov (United States)

    Sawada, Norimasa; Murata, Masaki; Kikuchi, Keisuke; Osanai, Makoto; Tobioka, Hirotoshi; Kojima, Takashi; Chiba, Hideki

    2003-09-01

    Tight junctions are intercellular junctions adjacent to the apical end of the lateral membrane surface. They have two functions, the barrier (or gate) function and the fence function. The barrier function of tight junctions regulates the passage of ions, water, and various macromolecules, even of cancer cells, through paracellular spaces. The barrier function is thus relevant to edema, jaundice, diarrhea, and blood-borne metastasis. On the other hand, the fence function maintains cell polarity. In other words, tight junctions work as a fence to prevent intermixing of molecules in the apical membrane with those in the lateral membrane. This function is deeply involved in cancer cell biology, in terms of loss of cell polarity. Of the proteins comprising tight junctions, integral membrane proteins occludin, claudins, and JAMs have been recently discovered. Of these molecules, claudins are exclusively responsible for the formation of tight-junction strands and are connected with the actin cytoskeleton mediated by ZO-1. Thus, both functions of tight junctions are dependent on the integrity of the actin cytoskeleton as well as ATP. Mutations in the claudin14 and the claudin16 genes result in hereditary deafness and hereditary hypomagnesemia, respectively. Some pathogenic bacteria and viruses target and affect the tight-junction function, leading to diseases. In this review, the relationship between tight junctions and human diseases is summarized.

  18. The TJ-II data acquisition system: an overview

    International Nuclear Information System (INIS)

    Vega, J.; Cremy, C.; Sanchez, E.; Portas, A.

    1999-01-01

    The data acquisition system for the TJ-II fusion machine has been developed to coordinate actions among the several experimental systems devoted to data capture and storage: instrumentation mainframes (VXI, VME, CAMAC), control systems of diagnostics and a host-centralized database. Connectivity between these elements is achieved through local area networks, which ensure both good connections and system growth capability. Three hundred VXI based digitizer channels have been developed for TJ-II diagnostics. They are completely software programmable and provide signal analog conditioning. In addition, some of them supply a programmable DSP for real time signal processing. Data will be stored in a central server using a special compression technique that allows compaction rates of over 80%. A specific application software has been developed to provide user interface for digitizer programming, signal visualization and data processing during TJ-II discharges. The software is an event based application that can be remotely launched from any X terminal An authentication mechanism restricts access to authorised users only. (orig.)

  19. Electron cyclotron emission measurements at the stellarator TJ-K

    Energy Technology Data Exchange (ETDEWEB)

    Sichardt, Gabriel; Ramisch, Mirko [Institut fuer Grenzflaechenverfahrenstechnik und Plasmatechnologie, Universitaet Stuttgart (Germany); Koehn, Alf [Max-Planck-Institut fuer Plasmaphysik, Garching (Germany)

    2016-07-01

    Electron temperature (T{sub e}) measurements in the magnetised plasmas of the stellarator TJ-K are currently performed by means of Langmuir probes. The use of these probes is restricted to relatively low temperatures and the measurement of temperature profiles requires the acquisition of the local current-voltage characteristics which limits strongly the sampling rate. As an alternative, T{sub e} can be measured using the electron cyclotron emission (ECE) that is generated by the gyration of electrons in magnetised plasmas. Magnetic field gradients in the plasma lead to a spatial distribution of emission frequencies and thus the measured intensity at a given frequency can be related to its point of origin. The T{sub e} dependence of the intensity then leads to a temperature profile along the line of sight for Maxwellian velocity distributions. A diagnostic system for T{sub e} measurements using ECE is currently being set up at TJ-K. When non-thermal electrons are present the emission spectrum changes dramatically. Therefore, the ECE can also be used to investigate the contribution of fast electrons to previously observed toroidal net currents in TJ-K. Simulations are used to examine the role of electron drift orbits in generating these currents.

  20. Search for Quasi-isodynamic Effects in TJ-II

    International Nuclear Information System (INIS)

    Guasp, J.; Liniers, M.

    2000-01-01

    The possibility of quasi-isodynamics effects (QID) in the TJ-II helical axis Stellarator has been explored maintaining the present setting for the toroidal field coils (TFC). In order to do this it has been necessary to implement a new method of calculation, using real space coordinates to follow the particle trajectories, instated the Boozer coordinates as was usual formerly. The result for the exploration of the flexibility diagram of TJ-II, including magnetic axis a shift effects, has been negative. It seems that there are not useful QID regions in TJ-II with the present setting of TFC carrying equal currents in all coils. Nevertheless, in spite of this negative result, the calculation in real space and, mainly, the grater number of configurations analysed, have produced a series of new important results, some of them unexpected. The influence of rational surfaces is very important. Optima and minima of confinement alternate at both sides of the rational values (mainly for the 1/2 by period) in a way very similar to the radial electric field resonance cases. This effect originates in the peculiar orbit topology in the presence of diffusion. Some lines of study are proposed to deal with this problem. Finally, the negative result of the QID search suggests the convenience to start a similar search without the restriction of equal currents on all the TEC. (Author) 18 refs

  1. Enhanced Protein Production in Escherichia coli by Optimization of Cloning Scars at the Vector-Coding Sequence Junction

    DEFF Research Database (Denmark)

    Mirzadeh, Kiavash; Martinez, Virginia; Toddo, Stephen

    2015-01-01

    are poorly expressed even when they are codon-optimized and expressed from vectors with powerful genetic elements. In this study, we show that poor expression can be caused by certain nucleotide sequences (e.g., cloning scars) at the junction between the vector and the coding sequence. Since these sequences...

  2. On the role of the gap junction protein Cx43 (GJA1 in human cardiac malformations with Fallot-pathology. a study on paediatric cardiac specimen.

    Directory of Open Access Journals (Sweden)

    Aida Salameh

    Full Text Available INTRODUCTION: Gap junction channels are involved in growth and differentiation. Therefore, we wanted to elucidate if the main cardiac gap junction protein connexin43 (GJA1 is altered in patients with Tetralogy of Fallot or double-outlet right ventricle of Fallot-type (62 patients referred to as Fallot compared to other cardiac anomalies (21 patients referred to as non-Fallot. Patients were divided into three age groups: 0-2years, 2-12years and >12years. Myocardial tissue samples were collected during corrective surgery and analysis of cell morphology, GJA1- and N-cadherin (CDH2-distribution, as well as GJA1 protein- and mRNA-expression was carried out. Moreover, GJA1-gene analysis of 16 patients and 20 healthy subjects was performed. RESULTS: Myocardial cell length and width were significantly increased in the oldest age group compared to the younger ones. GJA1 distribution changed significantly during maturation with the ratio of polar/lateral GJA1 increasing from 2.93±0.68 to 8.52±1.41. While in 0-2years old patients ∼6% of the lateral GJA1 was co-localised with CDH2 this decreased with age. Furthermore, the changes in cell morphology and GJA1-distribution were not due to the heart defect itself but were significantly dependent on age. Total GJA1 protein expression decreased during growing-up, whereas GJA1-mRNA remained unchanged. Sequencing of the GJA1-gene revealed only few heterozygous single nucleotide polymorphisms within the Fallot and the healthy control group. CONCLUSION: During maturation significant changes in gap junction remodelling occur which might be necessary for the growing and developing heart. In our study point mutations within the Cx43-gene could not be identified as a cause of the development of TOF.

  3. Disruption of Intracellular ATP Generation and Tight Junction Protein Expression during the Course of Brain Edema Induced by Subacute Poisoning of 1,2-Dichloroethane

    Directory of Open Access Journals (Sweden)

    Gaoyang Wang

    2018-01-01

    Full Text Available The aim of this study was to explore changes in intracellular ATP generation and tight junction protein expression during the course of brain edema induced by subacute poisoning of 1,2-dichloroethane (1,2-DCE. Mice were exposed to 1.2 g/m3 1,2-DCE for 3.5 h per day for 1, 2, or 3 days, namely group A, B, and C. Na+-K+-ATPase and Ca2+-ATPase activity, ATP and lactic acid content, intracellular free Ca2+ concentration and ZO-1 and occludin expression in the brain were measured. Results of present study disclosed that Ca2+-ATPase activities in group B and C, and Na+/K+-ATPase activity in group C decreased, whereas intracellular free Ca2+ concentrations in group B and C increased significantly compared with control. Moreover, ATP content decreased, whereas lactic acid content increased significantly in group C compared with control. On the other hand, expressions of ZO-1 and occludin at both the protein and gene levels in group B and C decreased significantly compared with control. In conclusion, findings from this study suggest that calcium overload and depressed expression of tight junction associated proteins, such as ZO-1 and occludin might play an important role in the early phase of brain edema formation induced by subacute poisoning of 1,2-DCE.

  4. Entamoeba histolytica EhCP112 Dislocates and Degrades Claudin-1 and Claudin-2 at Tight Junctions of the Intestinal Epithelium

    Directory of Open Access Journals (Sweden)

    Patricia Cuellar

    2017-08-01

    Full Text Available During intestinal invasion, Entamoeba histolytica opens tight junctions (TJs reflected by transepithelial electrical resistance (TEER dropping. To explore the molecular mechanisms underlying this, we studied in vitro and in vivo the damage produced by the recombinant E. histolytica cysteine protease (rEhCP112 on TJ functions and proteins. rEhCP112 reduced TEER in Caco-2 cells in a dose- and time-dependent manner; and EhCP112-overexpressing trophozoites provoked major epithelial injury compared to control trophozoites. rEhCP112 penetrated through the intercellular space, and consequently the ion flux increased and the TJs fence function was disturbed. However, macromolecular flux was not altered. Functional in vitro assays revealed specific association of rEhCP112 with claudin-1 and claudin-2, that are both involved in regulating ion flux and fence function. Of note, rEhCP112 did not interact with occludin that is responsible for regulating macromolecular flux. Moreover, rEhCP112 degraded and delocalized claudin-1, thus affecting interepithelial adhesion. Concomitantly, expression of the leaky claudin-2 at TJ, first increased and then it was degraded. In vivo, rEhCP112 increased intestinal epithelial permeability in the mouse colon, likely due to apical erosion and claudin-1 and claudin-2 degradation. In conclusion, we provide evidence that EhCP112 causes epithelial dysfunction by specifically altering claudins at TJ. Thus, EhCP112 could be a potential target for therapeutic approaches against amoebiasis.

  5. Additive Effects of Rebamipide Plus Proton Pump Inhibitors on the Expression of Tight Junction Proteins in a Rat Model of Gastro-Esophageal Reflux Disease.

    Science.gov (United States)

    Gweon, Tae-Geun; Park, Jong-Hyung; Kim, Byung-Wook; Choi, Yang Kyu; Kim, Joon Sung; Park, Sung Min; Kim, Chang Whan; Kim, Hyung-Gil; Chung, Jun-Won

    2018-01-15

    The aim of this study was to investigate the effects of rebamipide on tight junction proteins in the esophageal mucosa in a rat model of gastroesophageal reflux disease (GERD). GERD was created in rats by tying the proximal stomach. The rats were divided into a control group, a proton pump inhibitor (PPI) group, and a PPI plus rebamipide (PPI+R) group. Pantoprazole (5 mg/kg) was administered intraperitoneally to the PPI and PPI+R groups. An additional dose of rebamipide (100 mg/kg) was administered orally to the PPI+R group. Mucosal erosions, epithelial thickness, and leukocyte infiltration into the esophageal mucosa were measured in isolated esophagi 14 days after the procedure. A Western blot analysis was conducted to measure the expression of claudin-1, -3, and -4. The mean surface area of mucosal erosions, epithelial thickness, and leukocyte infiltration were lower in the PPI group and the PPI+R group than in the control group. Western blot analysis revealed that the expression of claudin-3 and -4 was significantly higher in the PPI+R group than in the control group. Rebamipide may exert an additive effect in combination with PPI to modify the tight junction proteins of the esophageal mucosa in a rat model of GERD. This treatment might be associated with the relief of GERD symptoms.

  6. Disorder effects in the t-J model

    International Nuclear Information System (INIS)

    Caprara, S.; De Palo, S.; Castellani, C.; Di Castro, C.; Grilli, M.

    1995-01-01

    We investigate the effects of disorder in the single-band t-J model, mainly devoting our analysis to the superconducting phases with d-wave or s-wave symmetry. We present evidence that, in the presence of strong correlation with reduced bandwidth and Van Hove singularity in the density of states, a self-consistent approach for the self-energy associated to the impurities is required. Numerical estimates of the reduction of the critical temperature with disorder are given. When a constant imaginary part of the self-energy is taken, avoiding self-consistency, the reduction of the critical temperature is overestimated

  7. Theoretical provisions for the discharge at TJ-1 (Preliminary study)

    International Nuclear Information System (INIS)

    Guasp, J.

    1981-01-01

    Using the transport code PLASMATOR a numerical study about the TJ-1 discharge (a Tokamak close to be installed at JEN) has been made, observing the behaviour under huge variations on the transport coefficients as well as on density and current. Noteworthy a scaling law of the kind τ E ∼n θ has been contested at not too high density, The model insensibility upon the initial values has been confirmed and the effects of variations on the recycling coefficient and the rate rise of current studied too. Finally comparisons with alternative models have been accomplished. (Author) 29 refs

  8. Neoclassical transport and radial electric fields in TJ-K

    International Nuclear Information System (INIS)

    Rahbarnia, K.; Greiner, F.; Ramisch, M.; Stroth, U.; Greiner, F.

    2003-01-01

    The neoclassical transport is investigated in the torsatron TJ-K, which is operated with a low-temperature plasma. In the low-collisionality regime neoclassical losses are not intrinsically ambipolar, leading to the formation of a radial electric field which acts on both neoclassical and turbulent transport. This electric field is measured with a combination of Langmuir and emissive probes. The data are compared with the ambipolar electric field calculated with an analytic model. The experimental fields are positive and larger than the calculated ones. Direct losses of the fast electrons might explain this discrepancy. (orig.)

  9. The NBI control system for the TJ-II

    Energy Technology Data Exchange (ETDEWEB)

    Carrasco, R. [Asociacion EURATOM-CIEMAT para Fusion, Avda. Complutense 22, 28040 Madrid (Spain)]. E-mail: ricardo.carrasco@ciemat.es; Liniers, M. [Asociacion EURATOM-CIEMAT para Fusion, Avda. Complutense 22, 28040 Madrid (Spain); Pacios, L. [Asociacion EURATOM-CIEMAT para Fusion, Avda. Complutense 22, 28040 Madrid (Spain); De la Pena, A. [Asociacion EURATOM-CIEMAT para Fusion, Avda. Complutense 22, 28040 Madrid (Spain); Lapayese, F. [Asociacion EURATOM-CIEMAT para Fusion, Avda. Complutense 22, 28040 Madrid (Spain); Wolfers, G. [Asociacion EURATOM-CIEMAT para Fusion, Avda. Complutense 22, 28040 Madrid (Spain); Alonso, J. [Asociacion EURATOM-CIEMAT para Fusion, Avda. Complutense 22, 28040 Madrid (Spain); Marcon, G. [Asociacion EURATOM-CIEMAT para Fusion, Avda. Complutense 22, 28040 Madrid (Spain); Fuentes, C. [Asociacion EURATOM-CIEMAT para Fusion, Avda. Complutense 22, 28040 Madrid (Spain)

    2006-07-15

    A description of the control system software and hardware architecture for the TJ-II Neutral Beam Injectors is given. The platform chosen is VMEbus, with controller boards running OS9 (Microware) real-time operating system. Three VME crates house several boards for performing analogue signal acquisition, signal conditioning, analogue voltage generation, digital input detection and digital output generation. A specific timing system for the injectors has been developed. At present, a user interface for monitoring and programming purposes is provided by html pages, using a web server running under the OS9 operating system. A few subsystems are now using a graphical user interface built using the Java programming language.

  10. The NBI control system for the TJ-II

    International Nuclear Information System (INIS)

    Carrasco, R.; Liniers, M.; Pacios, L.; De la Pena, A.; Lapayese, F.; Wolfers, G.; Alonso, J.; Marcon, G.; Fuentes, C.

    2006-01-01

    A description of the control system software and hardware architecture for the TJ-II Neutral Beam Injectors is given. The platform chosen is VMEbus, with controller boards running OS9 (Microware) real-time operating system. Three VME crates house several boards for performing analogue signal acquisition, signal conditioning, analogue voltage generation, digital input detection and digital output generation. A specific timing system for the injectors has been developed. At present, a user interface for monitoring and programming purposes is provided by html pages, using a web server running under the OS9 operating system. A few subsystems are now using a graphical user interface built using the Java programming language

  11. Demonstration of a III-nitride vertical-cavity surface-emitting laser with a III-nitride tunnel junction intracavity contact

    International Nuclear Information System (INIS)

    Leonard, J. T.; Young, E. C.; Yonkee, B. P.; Cohen, D. A.; Margalith, T.; Speck, J. S.; DenBaars, S. P.; Nakamura, S.

    2015-01-01

    We report on a III-nitride vertical-cavity surface-emitting laser (VCSEL) with a III-nitride tunnel junction (TJ) intracavity contact. The violet nonpolar VCSEL employing the TJ is compared to an equivalent VCSEL with a tin-doped indium oxide (ITO) intracavity contact. The TJ VCSEL shows a threshold current density (J th ) of ∼3.5 kA/cm 2 , compared to the ITO VCSEL J th of 8 kA/cm 2 . The differential efficiency of the TJ VCSEL is also observed to be significantly higher than that of the ITO VCSEL, reaching a peak power of ∼550 μW, compared to ∼80 μW for the ITO VCSEL. Both VCSELs display filamentary lasing in the current aperture, which we believe to be predominantly a result of local variations in contact resistance, which may induce local variations in refractive index and free carrier absorption. Beyond the analyses of the lasing characteristics, we discuss the molecular-beam epitaxy (MBE) regrowth of the TJ, as well as its unexpected performance based on band-diagram simulations. Furthermore, we investigate the intrinsic advantages of using a TJ intracavity contact in a VCSEL using a 1D mode profile analysis to approximate the threshold modal gain and general loss contributions in the TJ and ITO VCSEL

  12. Demonstration of a III-nitride vertical-cavity surface-emitting laser with a III-nitride tunnel junction intracavity contact

    Energy Technology Data Exchange (ETDEWEB)

    Leonard, J. T., E-mail: jtleona01@gmail.com; Young, E. C.; Yonkee, B. P.; Cohen, D. A.; Margalith, T.; Speck, J. S. [Materials Department, University of California, Santa Barbara, California 93106 (United States); DenBaars, S. P.; Nakamura, S. [Materials Department, University of California, Santa Barbara, California 93106 (United States); Department of Electrical and Computer Engineering, University of California, Santa Barbara, California 93106 (United States)

    2015-08-31

    We report on a III-nitride vertical-cavity surface-emitting laser (VCSEL) with a III-nitride tunnel junction (TJ) intracavity contact. The violet nonpolar VCSEL employing the TJ is compared to an equivalent VCSEL with a tin-doped indium oxide (ITO) intracavity contact. The TJ VCSEL shows a threshold current density (J{sub th}) of ∼3.5 kA/cm{sup 2}, compared to the ITO VCSEL J{sub th} of 8 kA/cm{sup 2}. The differential efficiency of the TJ VCSEL is also observed to be significantly higher than that of the ITO VCSEL, reaching a peak power of ∼550 μW, compared to ∼80 μW for the ITO VCSEL. Both VCSELs display filamentary lasing in the current aperture, which we believe to be predominantly a result of local variations in contact resistance, which may induce local variations in refractive index and free carrier absorption. Beyond the analyses of the lasing characteristics, we discuss the molecular-beam epitaxy (MBE) regrowth of the TJ, as well as its unexpected performance based on band-diagram simulations. Furthermore, we investigate the intrinsic advantages of using a TJ intracavity contact in a VCSEL using a 1D mode profile analysis to approximate the threshold modal gain and general loss contributions in the TJ and ITO VCSEL.

  13. Zonulin, regulation of tight junctions, and autoimmune diseases.

    Science.gov (United States)

    Fasano, Alessio

    2012-07-01

    Recent studies indicate that besides digestion and absorption of nutrients and water and electrolytes homeostasis, another key function of the intestine is to regulate the trafficking of environmental antigens across the host mucosal barrier. Intestinal tight junctions (TJs) create gradients for the optimal absorption and transport of nutrients and control the balance between tolerance and immunity to nonself antigens. To meet diverse physiological challenges, intestinal epithelial TJs must be modified rapidly and in a coordinated fashion by regulatory systems that orchestrate the state of assembly of the TJ multiprotein network. While considerable knowledge exists about TJ ultrastructure, relatively little is known about their physiological and pathophysiological regulation. Our discovery of zonulin, the only known physiologic modulator of intercellular TJs described so far, has increased our understanding of the intricate mechanisms that regulate the intestinal epithelial paracellular pathway and has led us to appreciate that its upregulation in genetically susceptible individuals leads to autoimmune diseases. © 2012 New York Academy of Sciences.

  14. Theoretical provisions for the discharge at TJ-1 (Preliminary study); Previsiones teoricas para la descarga del TJ-1 (Estudio preliminar)

    Energy Technology Data Exchange (ETDEWEB)

    Guasp, J

    1981-07-01

    Using the transport code PLASMATOR a numerical study about the TJ-1 discharge (a Tokamak close to be installed at JEN) has been made, observing the behaviour under huge variations on the transport coefficients as well as on density and current. Noteworthy a scaling law of the kind {tau}{sub E}{approx}n{sub {theta}} has been contested at not too high density, The model insensibility upon the initial values has been confirmed and the effects of variations on the recycling coefficient and the rate rise of current studied too. Finally comparisons with alternative models have been accomplished. (Author) 29 refs.

  15. Runaway transport studies in the TJ-I tokamak

    International Nuclear Information System (INIS)

    Rodriguez, L.; Vega, J.; Rodriguez-Yunta, A.; Castejon, F.; Pardo, C.; Navarro, A. P.

    1994-01-01

    Runaway diffusion coefficient, Dr, for TJ-I tokamak has been deduced using two different methods: In the first one, Dr is obtained using the steady state approach for values of the runaway confinement time Tτ r deduced from hard-X-ray bremsstrahlung spectra; in the second method, D r is deduced from sawteeth oscillations of HXR flux, and of SXR intensity, recorded simultaneously. Data have been taken in a scan with the toroidal magnetic field Bp Values obtained for Dr=[5-10] m 2 s ,decreasing with B Γ , are in both cases consistent. Assuming that magnetic turbulence is responsible for their transport, from D r we can deduce b/B Γ , the magnetic fluctuations level, and then infer the thermal conductivity coefficient χ c . The radial energy resolution of runaway electrons allow us to infer for b B Γ and χ c values at the plasma edge and for an inner position about r=a/2. Results are in good agreement with those ones obtained in TJ-I using probes, spectroscopic methods, power balance analysis and a coupled analysis for temperature and density pulses. (Author) 32 refs

  16. Experimental study of TJ-1 plasma using scattering and radiation emission techniques; Analisis experimental del plasma TJ-1 con tecnicas de scattering y emision de radiacion

    Energy Technology Data Exchange (ETDEWEB)

    Pardo, C; Zurro, B

    1987-07-01

    The Thomson scattering system of TJ-1 is described in detail. The radial profiles of Te and ne obtained in TJ-1 discharges are presented. This data make possible to deduce characteristic parameters of the plasma confinement in this machine, as energy confinement times, Zeff B. Using also radiation measurements (global and in the visible range) we obtained the particle confinement time and Zeff without non experimental assumptions. (Author) 52 refs.

  17. Web-based ground loop supervision system for the TJ-II Stellarator

    International Nuclear Information System (INIS)

    Pena, A. de la; Lapayese, F.; Pacios, L.; Carrasco, R.

    2005-01-01

    To minimize electromagnetic interferences in diagnostic and control signals, and to guarantee safe operation of TJ-II, ground loops must be avoided. In order to meet this goal, the whole grounding system of the TJ-II was split into multiple single branches that are connected at a single earth point located near the TJ-II structure in the torus hall. A real-time ground loop supervision system (GLSS) has been designed, manufactured and tested by the TJ-II control group for detecting unintentional short circuits between isolated grounded parts. A web server running on the real-time operating system OS-9 provides remote access to the real-time ground loops measurement. Ground loops monitoring and different operation modes can be configured via any web browser. This paper gives the detailed design of the whole TJ-II ground loop supervision system and its results during its operation

  18. Feasibility Study on a Neutral Beam Diagnostic Injector for TJ-II

    International Nuclear Information System (INIS)

    McCarthy, K. J.; Balbin, R.; Lopez-Fraguas, A.

    2003-01-01

    A diagnostic neutral beam system is proposed for the TJ-II stellarator. The main goal of installing such a system in TJ-II is to increase the signal to noise ratio and provide spatial resolution in diagnostic systems based on Charge Exchange Recombination Spectroscopy and Neutral Particle Analysis, while also opening up new opportunities for physics studies in this magnetically confined plasma device. After outlining the unique characteristics of the TJ-II and reviewing available diagnostic injector systems, the compact system selected for TJ-II is presented together with estimates of the resulting increased signal levels Finally other important aspects are discussed, in particular its location and orientation, as well as possible solutions to avoid perturbing the TJ-II magnetic configurations in the heliac device. (Author) 31 refs

  19. Feasibility Study on a Neutral Beam Diagnostic Injector for TJ-II

    Energy Technology Data Exchange (ETDEWEB)

    McCarthy, K. J.; Balbin, R.; Lopez-Fraguas, A.

    2003-07-01

    A diagnostic neutral beam system is proposed for the TJ-II stellarator. The main goal of installing such a system in TJ-II is to increase the signal to noise ratio and provide spatial resolution in diagnostic systems based on Charge Exchange Recombination Spectroscopy and Neutral Particle Analysis, while also opening up new opportunities for physics studies in this magnetically confined plasma device. After outlining the unique characteristics of the TJ-II and reviewing available diagnostic injector systems, the compact system selected for TJ-II is presented together with estimates of the resulting increased signal levels Finally other important aspects are discussed, in particular its location and orientation, as well as possible solutions to avoid perturbing the TJ-II magnetic configurations in the heliac device. (Author) 31 refs.

  20. Web-based ground loop supervision system for the TJ-II Stellarator

    Energy Technology Data Exchange (ETDEWEB)

    Pena, A. de la [Asociacion EURATOM-CIEMAT Para Fusion, Avd. Complutense 22, 28040 Madrid (Spain)]. E-mail: a.delapena@ciemat.es; Lapayese, F. [Asociacion EURATOM-CIEMAT Para Fusion, Avd. Complutense 22, 28040 Madrid (Spain); Pacios, L. [Asociacion EURATOM-CIEMAT Para Fusion, Avd. Complutense 22, 28040 Madrid (Spain); Carrasco, R. [Asociacion EURATOM-CIEMAT Para Fusion, Avd. Complutense 22, 28040 Madrid (Spain)

    2005-11-15

    To minimize electromagnetic interferences in diagnostic and control signals, and to guarantee safe operation of TJ-II, ground loops must be avoided. In order to meet this goal, the whole grounding system of the TJ-II was split into multiple single branches that are connected at a single earth point located near the TJ-II structure in the torus hall. A real-time ground loop supervision system (GLSS) has been designed, manufactured and tested by the TJ-II control group for detecting unintentional short circuits between isolated grounded parts. A web server running on the real-time operating system OS-9 provides remote access to the real-time ground loops measurement. Ground loops monitoring and different operation modes can be configured via any web browser. This paper gives the detailed design of the whole TJ-II ground loop supervision system and its results during its operation.

  1. Autonomous acquisition systems for TJ-II: controlling instrumentation with a fourth generation language

    International Nuclear Information System (INIS)

    Sanchez, E.; Portas, A.B.; Vega, J.; Agudo, J.M.; McCarthy, K.J.; Ruiz, M.; Barrera, E.; Lopez, S.

    2004-01-01

    Recently, 536 new acquisition channels, made-up of three different channel types, have been incorporated into the TJ-II data acquisition system (DAQ). Dedicated software has also been developed to permit experimentalists to program and control the data acquisition in these systems. The software has been developed using LabView and runs under the Windows 2000 operating system in both personal computer (PC) and PXI controllers. In addition, LabView software has been developed to control TJ-II VXI channels from a PC using a MXI connection. This new software environment will also aid future integration of acquisition channels into the TJ-II remote participation system. All of these acquisition devices work autonomously and are connected to the TJ-II central server via a local area network. In addition, they can be remotely controlled from the TJ-II control-room using Virtual Network Computing (VNC) software

  2. High performance as-grown and annealed high band gap tunnel junctions: Te behavior at the interface

    Energy Technology Data Exchange (ETDEWEB)

    Bedair, S. M., E-mail: bedair@ncsu.edu; Harmon, Jeffrey L.; Carlin, C. Zachary; Hashem Sayed, Islam E.; Colter, P. C. [Department of Electrical and Computer Engineering, North Carolina State University, Raleigh, North Carolina 27695 (United States)

    2016-05-16

    The performance of n{sup +}-InGaP(Te)/p{sup +}-AlGaAs(C) high band gap tunnel junctions (TJ) is critical for achieving high efficiency in multijunction photovoltaics. Several limitations for as grown and annealed TJ can be attributed to the Te doping of InGaP and its behavior at the junction interface. Te atoms in InGaP tend to get attached at step edges, resulting in a Te memory effect. In this work, we use the peak tunneling current (J{sub pk}) in this TJ as a diagnostic tool to study the behavior of the Te dopant at the TJ interface. Additionally, we used our understanding of Te behavior at the interface, guided by device modeling, to modify the Te source shut-off procedure and the growth rate. These modifications lead to a record performance for both the as-grown (2000 A/cm{sup 2}) and annealed (1000 A/cm{sup 2}) high band gap tunnel junction.

  3. Green tea polyphenols alleviate early BBB damage during experimental focal cerebral ischemia through regulating tight junctions and PKCalpha signaling.

    Science.gov (United States)

    Liu, Xiaobai; Wang, Zhenhua; Wang, Ping; Yu, Bo; Liu, Yunhui; Xue, Yixue

    2013-07-21

    It has been supposed that green tea polyphenols (GTPs) have neuroprotective effects on brain damage after brain ischemia in animal experiments. Little is known regarding GTPs' protective effects against the blood-brain barrier (BBB) disruption after ischemic stroke. We investigated the effects of GTPs on the expression of claudin-5, occludin, and ZO-1, and the corresponding cellular mechanisms involved in the early stage of cerebral ischemia. Male Wistar rats were subjected to a middle cerebral artery occlusion (MCAO) for 0, 30, 60, and 120 min. GTPs (400 mg/kg/day) or vehicle was administered by intragastric gavage twice a day for 30 days prior to MCAO. At different time points, the expression of claudin-5, occludin, ZO-1, and PKCα signaling pathway in microvessel fragments of cerebral ischemic tissue were evaluated. GTPs reduced BBB permeability at 60 min and 120 min after ischemia as compared with the vehicle group. Transmission electron microscopy also revealed that GTPs could reverse the opening of tight junction (TJ) barrier at 60 min and 120 min after MACO. The decreased mRNA and protein expression levels of claudin-5, occludin, and ZO-1 in microvessel fragments of cerebral ischemic tissue were significantly prevented by treatment with GTPs at the same time points after ischemia in rats. Furthermore, GTPs could attenuate the increase in the expression levels of PKCα mRNA and protein caused by cerebral ischemia. These results demonstrate that GTPs may act as a potential neuroprotective agent against BBB damage at the early stage of focal cerebral ischemia through the regulation of TJ and PKCα signaling.

  4. Immunohistochemical quantification of expression of a tight junction protein, claudin-7, in human lung cancer samples using digital image analysis method.

    Science.gov (United States)

    Lu, Zhe; Liu, Yi; Xu, Junfeng; Yin, Hongping; Yuan, Haiying; Gu, Jinjing; Chen, Yan-Hua; Shi, Liyun; Chen, Dan; Xie, Bin

    2018-03-01

    Tight junction proteins are correlated with cancer development. As the pivotal proteins in epithelial cells, altered expression and distribution of different claudins have been reported in a wide variety of human malignancies. We have previously reported that claudin-7 was strongly expressed in benign bronchial epithelial cells at the cell-cell junction while expression of claudin-7 was either altered with discontinued weak expression or completely absent in lung cancers. Based on these results, we continued working on the expression pattern of claudin-7 and its relationship with lung cancer development. We herein proposed a new Digital Image Classification, Fragmentation index, Morphological analysis (DICFM) method for differentiating the normal lung tissues and lung cancer tissues based on the claudin-7 immunohistochemical staining. Seventy-seven lung cancer samples were obtained from the Second Affiliated Hospital of Zhejiang University and claudin-7 immunohistochemical staining was performed. Based on C++ and Open Source Computer Vision Library (OpenCV, version 2.4.4), the DICFM processing module was developed. Intensity and fragmentation of claudin-7 expression, as well as the morphological parameters of nuclei were calculated. Evaluation of results was performed using Receiver Operator Characteristic (ROC) analysis. Agreement between these computational results and the results obtained by two pathologists was demonstrated. The intensity of claudin-7 expression was significantly decreased while the fragmentation was significantly increased in the lung cancer tissues compared to the normal lung tissues and the intensity was strongly positively associated with the differentiation of lung cancer cells. Moreover, the perimeters of the nuclei of lung cancer cells were significantly greater than that of the normal lung cells, while the parameters of area and circularity revealed no statistical significance. Taken together, our DICFM approach may be applied as an

  5. Acrolein Disrupts Tight Junction Proteins and Causes Endoplasmic Reticulum Stress-Mediated Epithelial Cell Death Leading to Intestinal Barrier Dysfunction and Permeability.

    Science.gov (United States)

    Chen, Wei-Yang; Wang, Min; Zhang, Jingwen; Barve, Shirish S; McClain, Craig J; Joshi-Barve, Swati

    2017-12-01

    Increasing evidence suggests that environmental and dietary factors can affect intestinal epithelial integrity leading to gut permeability and bacterial translocation. Intestinal barrier dysfunction is a pathogenic process associated with many chronic disorders. Acrolein is an environmental and dietary pollutant and a lipid-derived endogenous metabolite. The impact of acrolein on the intestine has not been investigated before and is evaluated in this study, both in vitro and in vivo. Our data demonstrate that oral acrolein exposure in mice caused damage to the intestinal epithelial barrier, resulting in increased permeability and subsequently translocation of bacterial endotoxin-lipopolysaccharide into the blood. Similar results were seen in vitro using established Caco-2 cell monolayers wherein acrolein decreased barrier function and increased permeability. Acrolein also caused the down-regulation and/or redistribution of three representative tight junction proteins (ie, zonula occludens-1, Occludin, Claudin-1) that critically regulate epithelial paracellular permeability. In addition, acrolein induced endoplasmic reticulum stress-mediated death of epithelial cells, which is an important mechanism contributing to intestinal barrier damage/dysfunction, and gut permeability. Overall, we demonstrate that exposure to acrolein affects the intestinal epithelium by decrease/redistribution of tight junction proteins and endoplasmic reticulum stress-mediated epithelial cell death, thereby resulting in loss of barrier integrity and function. Our findings highlight the adverse consequences of environmental and dietary pollutants on intestinal barrier integrity/function with relevance to gut permeability and the development of disease. Copyright © 2017 American Society for Investigative Pathology. Published by Elsevier Inc. All rights reserved.

  6. Downregulation of tight junction-associated MARVEL protein marvelD3 during epithelial-mesenchymal transition in human pancreatic cancer cells.

    Science.gov (United States)

    Kojima, Takashi; Takasawa, Akira; Kyuno, Daisuke; Ito, Tatsuya; Yamaguchi, Hiroshi; Hirata, Koichi; Tsujiwaki, Mitsuhiro; Murata, Masaki; Tanaka, Satoshi; Sawada, Norimasa

    2011-10-01

    The novel tight junction protein marvelD3 contains a conserved MARVEL (MAL and related proteins for vesicle trafficking and membrane link) domain like occludin and tricellulin. However, little is yet known about the detailed role and regulation of marvelD3 in normal epithelial cells and cancer cells, including pancreatic cancer. In the present study, we investigated marvelD3 expression in well and poorly differentiated human pancreatic cancer cell lines and normal pancreatic duct epithelial cells in which the hTERT gene was introduced into human pancreatic duct epithelial cells in primary culture, and the changes of marvelD3 during Snail-induced epithelial-mesenchymal transition (EMT) under hypoxia, TGF-β treatment and knockdown of FOXA2 in well differentiated pancreatic cancer HPAC cells. MarvelD3 was transcriptionally downregulated in poorly differentiated pancreatic cancer cells and during Snail-induced EMT of pancreatic cancer cells in which Snail was highly expressed and the fence function downregulated, whereas it was maintained in well differentiated human pancreatic cancer cells and normal pancreatic duct epithelial cells. Depletion of marvelD3 by siRNAs in HPAC cells resulted in downregulation of barrier functions indicated as a decrease in transepithelial electric resistance and an increase of permeability to fluorescent dextran tracers, whereas it did not affect fence function of tight junctions. In conclusion, marvelD3 is transcriptionally downregulated in Snail-induced EMT during the progression for the pancreatic cancer. Copyright © 2011 Elsevier Inc. All rights reserved.

  7. Identification of new interacting partners of the shuttling protein ubinuclein (Ubn-1)

    Energy Technology Data Exchange (ETDEWEB)

    Lupo, Julien [Unit of Virus Host Cell Interactions (UVHCI), UMI 3265 UJF-EMBL-CNRS, 6 rue Jules Horowitz, BP 181, F-38042 Grenoble Cedex 9 (France); CHU de Grenoble, BP217, 38043 Grenoble Cedex 9 (France); Conti, Audrey [Unit of Virus Host Cell Interactions (UVHCI), UMI 3265 UJF-EMBL-CNRS, 6 rue Jules Horowitz, BP 181, F-38042 Grenoble Cedex 9 (France); Sueur, Charlotte [Unit of Virus Host Cell Interactions (UVHCI), UMI 3265 UJF-EMBL-CNRS, 6 rue Jules Horowitz, BP 181, F-38042 Grenoble Cedex 9 (France); CHU de Grenoble, BP217, 38043 Grenoble Cedex 9 (France); Coly, Pierre-Alain [Unit of Virus Host Cell Interactions (UVHCI), UMI 3265 UJF-EMBL-CNRS, 6 rue Jules Horowitz, BP 181, F-38042 Grenoble Cedex 9 (France); Coute, Yohann [CEA, IRTSV, Laboratoire Biologie a Grande Echelle, F-38054 Grenoble (France); INSERM, U1038, F-38054 Grenoble (France); Universite Joseph Fourier, Grenoble 1, F-38000 Grenoble Cedex 09 (France); Hunziker, Walter [Institute of Molecular and Cell Biology, Epithelial Cell Biology Laboratory, Singapore 1386473 (Singapore); Burmeister, Wim P. [Unit of Virus Host Cell Interactions (UVHCI), UMI 3265 UJF-EMBL-CNRS, 6 rue Jules Horowitz, BP 181, F-38042 Grenoble Cedex 9 (France); Germi, Raphaelle [Unit of Virus Host Cell Interactions (UVHCI), UMI 3265 UJF-EMBL-CNRS, 6 rue Jules Horowitz, BP 181, F-38042 Grenoble Cedex 9 (France); CHU de Grenoble, BP217, 38043 Grenoble Cedex 9 (France); Manet, Evelyne; Gruffat, Henri [INSERM U758, Unite de Virologie humaine, Lyon, 46 allee d' Italie F-69007 France (France); Ecole Normale Superieure de Lyon, F-69007 France (France); Universite Lyon1, F-69007, Lyon (France); and others

    2012-03-10

    We have previously characterized ubinuclein (Ubn-1) as a NACos (Nuclear and Adherent junction Complex components) protein which interacts with viral or cellular transcription factors and the tight junction (TJ) protein ZO-1. The purpose of the present study was to get more insights on the binding partners of Ubn-1, notably those present in the epithelial junctions. Using an in vivo assay of fluorescent protein-complementation assay (PCA), we demonstrated that the N-terminal domains of the Ubn-1 and ZO-1 proteins triggered a functional interaction inside the cell. Indeed, expression of both complementary fragments of venus fused to the N-terminal parts of Ubn-1 and ZO-1 was able to reconstitute a fluorescent venus protein. Furthermore, nuclear expression of the chimeric Ubn-1 triggered nuclear localization of the chimeric ZO-1. We could localize this interaction to the PDZ2 domain of ZO-1 using an in vitro pull-down assay. More precisely, a 184-amino acid region (from amino acids 39 to 223) at the N-terminal region of Ubn-1 was responsible for the interaction with the PDZ2 domain of ZO-1. Co-imunoprecipitation and confocal microscopy experiments also revealed the tight junction protein cingulin as a new interacting partner of Ubn-1. A proteomic approach based on mass spectrometry analysis (MS) was then undertaken to identify further binding partners of GST-Ubn-1 fusion protein in different subcellular fractions of human epithelial HT29 cells. LYRIC (Lysine-rich CEACAM1-associated protein) and RACK-1 (receptor for activated C-kinase) proteins were validated as bona fide interacting partners of Ubn-1. Altogether, these results suggest that Ubn-1 is a scaffold protein influencing protein subcellular localization and is involved in several processes such as cell-cell contact signalling or modulation of gene activity.

  8. Searching for patterns in TJ-II time evolution signals

    International Nuclear Information System (INIS)

    Farias, G.; Dormido-Canto, S.; Vega, J.; Sanchez, J.; Duro, N.; Dormido, R.; Ochando, M.; Santos, M.; Pajares, G.

    2006-01-01

    Since fusion plasma experiments generate hundreds of signals, it is important for their analysis to have automatic mechanisms for searching for similarities and retrieving specific data from the signal database. This paper describes a technique for searching in the TJ-II database that combines support vector machines and similarity query methods. Firstly, plasma signals are pre-processed by wavelet transform or discrete Fourier transform to reduce the dimensionality of the problem and to extract their main features. Secondly, support vector machines are used to classify a set of signals by reference to an input signal. Finally, similarity query methods (Euclidean distance and bounding envelope) are used to search the set of signals that best matches the input signal

  9. Radial electrical field effects in TJ-II. (Preliminary study)

    International Nuclear Information System (INIS)

    Guasp, J.

    1996-01-01

    The influence of the radial electric field upon the neoclassical transport coefficients of TJ-II helical axis Stellarator has been calculated as well on the microwave heating stage (ECRH) as on the neutral injection one (NBI). The influence of the solutions for the self-consistent ambipolar field on confinement times and temperatures has been studied by means of a zero-dimensional energy balance. The simultaneous presence of two roots, the electronic and the ionic one, is observed for the ECRH phase, while for NBI only the ionic root appears, although with a strong field intensity that could produce a favourable effect on confinement. The interest and need of the extension of these calculations to include radial profile effects by using spatial dependent transport codes in stressed

  10. Perturbative Heat Transport Experiments on TJ-II

    International Nuclear Information System (INIS)

    Eguilor, S.; Castejon, F.; Luna, E. de la; Cappa, A.; Likin, K.; Fernandez, A.; Tj-II, T.

    2002-01-01

    Heat wave experiments are performed on TJ-II stellarator plasmas to estimate both heat diffusivity and power deposition profiles. High frequency ECRH modulation experiments are used to obtain the power deposition profiles, which is observed to be wider and duller than estimated by tracing techniques. The causes of this difference are discussed in the paper. Fourier analysis techniques are used to estimate the heat diffusivity in low frequency ECRH modulation experiments. This include the power deposition profile as a new ingredient. ECHR switch on/off experiments are exploited to obtain power deposition and heat diffusivities profile. Those quantities are compared with the obtained by modulation experiments and transport analysis, showing a good agreement. (Author) 18 refs

  11. Power supply for the Spanish Stellarator TJ-II

    International Nuclear Information System (INIS)

    Almoguera, L. de; Blaumoser, M.; Mendez, P.; Kirpitchev, I.

    1995-01-01

    TJ-II is a flexible, medium size, heliac under construction at the EURATOM-CIEMAT Association in Madrid, Spain. Its main data are: major plasma radius: 1,5 m; minor plasma dimension: 0,2 m by 0,4 m; toroidal magnetic field: 1 T; rotational transform: 0,9 to 2,5; shear: -1% to 10 %; magnetic well: 0 to 6 %. This device requires an entirely new electrical system to power its magnetic field coils and the plasma heating systems. As the local grid is not powerful enough, a pulse power system will be installed which consists of a DC-pony motor, a synchronous generator and seven 12-pulse controlled thyristor rectifiers. A contract has been placed with JEMA, San Sebastian, Spain, as the general contractor which provides the entire power supply system as a turn-key package. (orig.)

  12. Anomalous superconductivity in the tJ model; moment approach

    DEFF Research Database (Denmark)

    Sørensen, Mads Peter; Rodriguez-Nunez, J.J.

    1997-01-01

    By extending the moment approach of Nolting (Z, Phys, 225 (1972) 25) in the superconducting phase, we have constructed the one-particle spectral functions (diagonal and off-diagonal) for the tJ model in any dimensions. We propose that both the diagonal and the off-diagonal spectral functions...... Hartree shift which in the end result enlarges the bandwidth of the free carriers allowing us to take relative high values of J/t and allowing superconductivity to live in the T-c-rho phase diagram, in agreement with numerical calculations in a cluster, We have calculated the static spin susceptibility......, chi(T), and the specific heat, C-v(T), within the moment approach. We find that all the relevant physical quantities show the signature of superconductivity at T-c in the form of kinks (anomalous behavior) or jumps, for low density, in agreement with recent published literature, showing a generic...

  13. Perturbative Heat Transport Experiments on TJ-II

    Energy Technology Data Exchange (ETDEWEB)

    Eguilor, S.; Castejon, F.; Luna, E. de la; Cappa, A.; Likin, K.; Fernandez, A.; Tj-II, T.

    2002-07-01

    Heat wave experiments are performed on TJ-II stellarator plasmas to estimate both heat diffusivity and power deposition profiles. High frequency ECRH modulation experiments are used to obtain the power deposition profiles, which is observed to be wider and duller than estimated by tracing techniques. The causes of this difference are discussed in the paper. Fourier analysis techniques are used to estimate the heat diffusivity in low frequency ECRH modulation experiments. This include the power deposition profile as a new ingredient. ECHR switch on/off experiments are exploited to obtain power deposition and heat diffusivities profile. Those quantities are compared with the obtained by modulation experiments and transport analysis, showing a good agreement. (Author) 18 refs.

  14. Ischemic preconditioning enhances integrity of coronary endothelial tight junctions

    International Nuclear Information System (INIS)

    Li, Zhao; Jin, Zhu-Qiu

    2012-01-01

    Highlights: ► Cardiac tight junctions are present between coronary endothelial cells. ► Ischemic preconditioning preserves the structural and functional integrity of tight junctions. ► Myocardial edema is prevented in hearts subjected to ischemic preconditioning. ► Ischemic preconditioning enhances translocation of ZO-2 from cytosol to cytoskeleton. -- Abstract: Ischemic preconditioning (IPC) is one of the most effective procedures known to protect hearts against ischemia/reperfusion (IR) injury. Tight junction (TJ) barriers occur between coronary endothelial cells. TJs provide barrier function to maintain the homeostasis of the inner environment of tissues. However, the effect of IPC on the structure and function of cardiac TJs remains unknown. We tested the hypothesis that myocardial IR injury ruptures the structure of TJs and impairs endothelial permeability whereas IPC preserves the structural and functional integrity of TJs in the blood–heart barrier. Langendorff hearts from C57BL/6J mice were prepared and perfused with Krebs–Henseleit buffer. Cardiac function, creatine kinase release, and myocardial edema were measured. Cardiac TJ function was evaluated by measuring Evans blue-conjugated albumin (EBA) content in the extravascular compartment of hearts. Expression and translocation of zonula occludens (ZO)-2 in IR and IPC hearts were detected with Western blot. A subset of hearts was processed for the observation of ultra-structure of cardiac TJs with transmission electron microscopy. There were clear TJs between coronary endothelial cells of mouse hearts. IR caused the collapse of TJs whereas IPC sustained the structure of TJs. IR increased extravascular EBA content in the heart and myocardial edema but decreased the expression of ZO-2 in the cytoskeleton. IPC maintained the structure of TJs. Cardiac EBA content and edema were reduced in IPC hearts. IPC enhanced the translocation of ZO-2 from cytosol to cytoskeleton. In conclusion, TJs occur in

  15. Claudin-8d is a cortisol-responsive barrier protein in the gill epithelium of trout.

    Science.gov (United States)

    Kolosov, Dennis; Kelly, Scott P

    2017-10-01

    The influence of claudin (Cldn) 8 tight junction (TJ) proteins on cortisol-mediated alterations in gill epithelium permeability was examined using a primary cultured trout gill epithelium model. Genes encoding three Cldn-8 proteins ( cldn-8b, -8c and -8d ) have been identified in trout and all are expressed in the model gill epithelium. Cortisol treatment 'tightened' the gill epithelium, as indicated by increased transepithelial resistance (TER) and reduced paracellular [ 3 H]polyethylene glycol (MW 400 Da; PEG-400) flux. This occurred in association with elevated cldn-8d mRNA abundance, but no alterations in cldn-8b and -8c mRNA abundance were observed. Transcriptional knockdown (KD) of cldn-8d inhibited a cortisol-induced increase in Cldn-8d abundance and reduced the 'epithelium tightening' effect of cortisol in association with increased paracellular PEG-400 flux. Under simulated in vivo conditions (i.e. apical freshwater), cldn-8d KD hindered a cortisol-mediated reduction in basolateral to apical Na + and Cl - flux (i.e. reduced the ability of cortisol to mitigate ion loss). However, cldn-8d KD did not abolish the tightening effect of cortisol on the gill epithelium. This is likely due, in part, to the effect of cortisol on genes encoding other TJ proteins, which in some cases appeared to exhibit a compensatory response. Data support the idea that Cldn-8d is a barrier protein of the gill epithelium TJ that contributes significantly to corticosteroid-mediated alterations in gill epithelium permeability. © 2017 Society for Endocrinology.

  16. Structure modeling and mutational analysis of gap junction beta 2 ...

    African Journals Online (AJOL)

    Yomi

    2012-04-03

    Apr 3, 2012 ... Three dimensional (3 D) structure is very useful for understanding biological functions. Gap junction beta 2 (GJB2), human gene encoding for gap junction beta 2 protein is involved in ... Research in deafness became real.

  17. Effect of low and staggered gap quantum wells inserted in GaAs tunnel junctions

    Science.gov (United States)

    Louarn, K.; Claveau, Y.; Marigo-Lombart, L.; Fontaine, C.; Arnoult, A.; Piquemal, F.; Bounouh, A.; Cavassilas, N.; Almuneau, G.

    2018-04-01

    In this article, we investigate the impact of the insertion of either a type I InGaAs or a type II InGaAs/GaAsSb quantum well on the performances of MBE-grown GaAs tunnel junctions (TJs). The devices are designed and simulated using a quantum transport model based on the non-equilibrium Green’s function formalism and a 6-band k.p Hamiltonian. We experimentally observe significant improvements of the peak tunneling current density on both heterostructures with a 460-fold increase for a moderately doped GaAs TJ when the InGaAs QW is inserted at the junction interface, and a 3-fold improvement on a highly doped GaAs TJ integrating a type II InGaAs/GaAsSb QW. Thus, the simple insertion of staggered band lineup heterostructures enables us to reach a tunneling current well above the kA cm‑2 range, equivalent to the best achieved results for Si-doped GaAs TJs, implying very interesting potential for TJ-based components, such as multi-junction solar cells, vertical cavity surface emitting lasers and tunnel-field effect transistors.

  18. Export of a Toxoplasma gondii rhoptry neck protein complex at the host cell membrane to form the moving junction during invasion.

    Directory of Open Access Journals (Sweden)

    Sébastien Besteiro

    2009-02-01

    Full Text Available One of the most conserved features of the invasion process in Apicomplexa parasites is the formation of a moving junction (MJ between the apex of the parasite and the host cell membrane that moves along the parasite and serves as support to propel it inside the host cell. The MJ was, up to a recent period, completely unknown at the molecular level. Recently, proteins originated from two distinct post-Golgi specialised secretory organelles, the micronemes (for AMA1 and the neck of the rhoptries (for RON2/RON4/RON5 proteins, have been shown to form a complex. AMA1 and RON4 in particular, have been localised to the MJ during invasion. Using biochemical approaches, we have identified RON8 as an additional member of the complex. We also demonstrated that all RON proteins are present at the MJ during invasion. Using metabolic labelling and immunoprecipitation, we showed that RON2 and AMA1 were able to interact in the absence of the other members. We also discovered that all MJ proteins are subjected to proteolytic maturation during trafficking to their respective organelles and that they could associate as non-mature forms in vitro. Finally, whereas AMA1 has previously been shown to be inserted into the parasite membrane upon secretion, we demonstrated, using differential permeabilization and loading of RON-specific antibodies into the host cell, that the RON complex is targeted to the host cell membrane, where RON4/5/8 remain associated with the cytoplasmic face. Globally, these results point toward a model of MJ organization where the parasite would be secreting and inserting interacting components on either side of the MJ, both at the host and at its own plasma membranes.

  19. Application for TJ-II Signals Visualization: User's Guide; Aplicacion para la Visualizacion de Senales de TJ-II: Guia del Usuario

    Energy Technology Data Exchange (ETDEWEB)

    Sanchez, E.; Portas, A. B.; Vega, J. [Ciemat, Madrid (Spain)

    2000-07-01

    In this documents are described the functionalities of the application developed by the Data Acquisition Group for TJ-II signal visualization. There are two versions of the application, the On-line version, used for signal visualization during TJ-II operation, and the Off-line version, used for signal visualization without TJ-II operation. Both versions of the application consist in a graphical user interface developed for X/Motif, in which most of the actions can be done using the mouse buttons. The functionalities of both versions of the application are described in this user's guide, beginning at the application start-up and explaining in detail all the options that it provides and the actions that can be done with each graphic control. (Author) 8 refs.

  20. Description of the heliac TJ-II and its ECRH system

    International Nuclear Information System (INIS)

    Perea, A.; Martin, R.; Alvarez Rivas, J.L.

    1986-01-01

    This paper describes the main engineering aspects of the flexible heliac TJ-II, as well as the ECRH scheme that will be used for initial operation. Previous papers have described the physics of the device

  1. Comparison of nonpolar III-nitride vertical-cavity surface-emitting lasers with tunnel junction and ITO intracavity contacts

    KAUST Repository

    Leonard, J. T.

    2016-03-01

    We report on the lasing of III-nitride nonpolar, violet, vertical-cavity surface-emitting lasers (VCSELs) with III-nitride tunnel-junction (TJ) intracavity contacts and ion implanted apertures (IIAs). The TJ VCSELs are compared to similar VCSELs with tin-doped indium oxide (ITO) intracavity contacts. Prior to analyzing device results, we consider the relative advantages of III-nitride TJs for blue and green emitting VCSELs. The TJs are shown to be most advantageous for violet and UV VCSELs, operating near or above the absorption edge for ITO, as they significantly reduce the total internal loss in the cavity. However, for longer wavelength III-nitride VCSELs, TJs primarily offer the advantage of improved cavity design flexibility, allowing one to make the p-side thicker using a thick n-type III-nitride TJ intracavity contact. This offers improved lateral current spreading and lower loss, compare to using ITO and p-GaN, respectively. These aspects are particularly important for achieving high-power CW VCSELs, making TJs the ideal intracavity contact for any III-nitride VCSEL. A brief overview of III-nitride TJ growth methods is also given, highlighting the molecular-beam epitaxy (MBE) technique used here. Following this overview, we compare 12 mu m aperture diameter, violet emitting, TJ and ITO VCSEL experimental results, which demonstrate the significant improvement in differential efficiency and peak power resulting from the reduced loss in the TJ design. Specifically, the TJ VCSEL shows a peak power of similar to 550 mu W with a threshold current density of similar to 3.5 kA/cm(2), while the ITO VCSELs show peak powers of similar to 80 mu W and threshold current densities of similar to 7 kA/cm

  2. Comparison of nonpolar III-nitride vertical-cavity surface-emitting lasers with tunnel junction and ITO intracavity contacts

    KAUST Repository

    Leonard, J. T.; Young, E. C.; Yonkee, B. P.; Cohen, D. A.; Shen, Chao; Margalith, T.; Ng, Tien Khee; Denbaars, S. P.; Ooi, Boon S.; Speck, J. S.; Nakamura, S.

    2016-01-01

    We report on the lasing of III-nitride nonpolar, violet, vertical-cavity surface-emitting lasers (VCSELs) with III-nitride tunnel-junction (TJ) intracavity contacts and ion implanted apertures (IIAs). The TJ VCSELs are compared to similar VCSELs with tin-doped indium oxide (ITO) intracavity contacts. Prior to analyzing device results, we consider the relative advantages of III-nitride TJs for blue and green emitting VCSELs. The TJs are shown to be most advantageous for violet and UV VCSELs, operating near or above the absorption edge for ITO, as they significantly reduce the total internal loss in the cavity. However, for longer wavelength III-nitride VCSELs, TJs primarily offer the advantage of improved cavity design flexibility, allowing one to make the p-side thicker using a thick n-type III-nitride TJ intracavity contact. This offers improved lateral current spreading and lower loss, compare to using ITO and p-GaN, respectively. These aspects are particularly important for achieving high-power CW VCSELs, making TJs the ideal intracavity contact for any III-nitride VCSEL. A brief overview of III-nitride TJ growth methods is also given, highlighting the molecular-beam epitaxy (MBE) technique used here. Following this overview, we compare 12 mu m aperture diameter, violet emitting, TJ and ITO VCSEL experimental results, which demonstrate the significant improvement in differential efficiency and peak power resulting from the reduced loss in the TJ design. Specifically, the TJ VCSEL shows a peak power of similar to 550 mu W with a threshold current density of similar to 3.5 kA/cm(2), while the ITO VCSELs show peak powers of similar to 80 mu W and threshold current densities of similar to 7 kA/cm

  3. Experimental study of TJ-1 plasma using scattering and radiation emission techniques

    International Nuclear Information System (INIS)

    Pardo, C.; Zurro, B.

    1987-01-01

    The Thomson scattering system of TJ-1 is described in detail. The radial profiles of Te and ne obtained in TJ-1 discharges are presented. This data make possible to deduce characteristic parameters of the plasma confinement in this machine, as energy confinement times, Zeff B. Using also radiation measurements (global and in the visible range) we obtained the particle confinement time and Zeff without non experimental assumptions. (Author) 52 refs

  4. Study of TJ-1 Tokamak plasmas with Thomson scattering and radiation diagnostics

    International Nuclear Information System (INIS)

    Pardo, C.; Zurro, B.

    1987-06-01

    The Thomson scattering system of TJ-1 is described in detail. The radial profiles of T e and n e obtained in TJ-1 discharges are presented. This data makes possible to deduce characteristic parameters of the plasma confinement in this machine, as energy confinement times, Z eff B. Using also radiation measurements (global and in the visible range) we obtained the particle confinement time and Z eff without non experimental assumptions. (author) 56 figs., 52 refs

  5. Automated System for Control of the Vacuum Diagnostic System for the TJ-II

    International Nuclear Information System (INIS)

    Lopez Sanchez, A.; Montoro Peinado, A.; Encabo Fernandez, J.; Gama de la Serrano, J.; Sanchez Sarabia, E.

    1999-12-01

    This report describes the monitoring and remote control systems belonging to the high vacuum systems of the TJ-II diagnostics. These systems are part of each diagnostic and their control has been integrated into the automata that carries out this task. All the controllers are connected through a Profibus network, so as to interchange data between themselves as well as between the general system of TJ-II. (Author)

  6. Recent results with NBI plasmas in TJ-II stellarator

    Energy Technology Data Exchange (ETDEWEB)

    Liniers, M.; Ascasibar, E.; Estrada, T.; Tabares, F. L.; Acedo, M.; Alonso, J.; Balbin, R.; Blanco, B.; Branas, B.; Cappa, A.; Carrasco, R.; Castejon, F.; Fernandez, A.; Fontdecaba, J. M.; Fuentes, C.; Garcia, A.; Garcia-Cortes, I.; Guasp, J.; Hidalgo, A.; Hidalgo, C.; Jimenez, R.; Lopez-Bruna, D.; Lopez-Fraguas, A.; Marcon, G.; McCarthy, K. J.; Medina, F.; Medrano, M.; Ochando, M.; Pastor, I.; Pedrosa, M. A.; Rapisarda, D.; Sanchez, E.; Sanchez, M.; Sanchez, J.; Tafalla, D.; Wolfers, G.; Zurro, B.

    2005-07-01

    During the last experimental campaign Neutral Beam Injection into TJ-II plasmas has been available, with a single H0 beam aiming tangentially in the Co-direction. As the ion source conditioning was improved along the campaign, the injected power increased from 200 kW to 400 kW port-through, and the beam energy was raised from 26 kV to 30 kV [1]. Target plasmas are created by ECR heating, using two gyrotrons of 200 kW power, at the second harmonic frequency (53 GHz). The injection direction of the microwaves can be steered by means of movable mirrors placed inside the vacuum chamber, making it possible to vary the power deposition region from the plasma core (on-axis) to the outer regions near ?=0.5 (off-axis). The plasma temperature and density profiles obtained with on-axis or off axis ECRH are seen to differ widely, allowing us to study the neutral beam absorption in two qualitatively different plasma target scenarios. Other factors affecting the plasma profiles have also been investigated, such as the magnetic configuration or the OH-driven current. TJ-II has the capability of varying the magnetic configuration by changing the ratio of the current through the circular and helical conductors that form the Central Conductor. The iota values can be swept between 0.9 and 2.2 and the magnetic well between -1% and 6% giving rise to configurations with different confinement properties. A negative OH driven current has the effect of increasing the magnetic shear value, allowing low-order rationals in the central region which have been seen to modify density profiles in ECH plasmas. In most NBI discharges the central plasma density increases continuously from ECH typical values below 1.1 x 10 19 m-3 up to 6.5 x 10 19 m-3, as the beam is injected, until a thermal collapse that terminates the discharge is reached. So far, density control with NBI plasmas has not been achieved, although an improved behaviour is observed with wall cleaning. (Author)

  7. Recent results with NBI plasmas in TJ-II stellarator

    International Nuclear Information System (INIS)

    Liniers, M.; Ascasibar, E.; Estrada, T.; Tabares, F. L.; Acedo, M.; Alonso, J.; Balbin, R.; Blanco, B.; Branas, B.; Cappa, A.; Carrasco, R.; Castejon, F.; Fernandez, A.; Fontdecaba, J. M.; Fuentes, C.; Garcia, A.; Garcia-Cortes, I.; Guasp, J.; Hidalgo, A.; Hidalgo, C.; Jimenez, R.; Lopez-Bruna, D.; Lopez-Fraguas, A.; Marcon, G.; McCarthy, K. J.; Medina, F.; Medrano, M.; Ochando, M.; Pastor, I.; Pedrosa, M. A.; Rapisarda, D.; Sanchez, E.; Sanchez, M.; Sanchez, J.; Tafalla, D.; Wolfers, G.; Zurro, B.

    2005-01-01

    During the last experimental campaign Neutral Beam Injection into TJ-II plasmas has been available, with a single H0 beam aiming tangentially in the Co-direction. As the ion source conditioning was improved along the campaign, the injected power increased from 200 kW to 400 kW port-through, and the beam energy was raised from 26 kV to 30 kV [1]. Target plasmas are created by ECR heating, using two gyrotrons of 200 kW power, at the second harmonic frequency (53 GHz). The injection direction of the microwaves can be steered by means of movable mirrors placed inside the vacuum chamber, making it possible to vary the power deposition region from the plasma core (on-axis) to the outer regions near ?=0.5 (off-axis). The plasma temperature and density profiles obtained with on-axis or off axis ECRH are seen to differ widely, allowing us to study the neutral beam absorption in two qualitatively different plasma target scenarios. Other factors affecting the plasma profiles have also been investigated, such as the magnetic configuration or the OH-driven current. TJ-II has the capability of varying the magnetic configuration by changing the ratio of the current through the circular and helical conductors that form the Central Conductor. The iota values can be swept between 0.9 and 2.2 and the magnetic well between -1% and 6% giving rise to configurations with different confinement properties. A negative OH driven current has the effect of increasing the magnetic shear value, allowing low-order rationals in the central region which have been seen to modify density profiles in ECH plasmas. In most NBI discharges the central plasma density increases continuously from ECH typical values below 1.1 x 10 19 m-3 up to 6.5 x 10 19 m-3, as the beam is injected, until a thermal collapse that terminates the discharge is reached. So far, density control with NBI plasmas has not been achieved, although an improved behaviour is observed with wall cleaning. (Author)

  8. Impact of 7,12-dimethylbenz[a]anthracene exposure on connexin gap junction proteins in cultured rat ovaries

    Energy Technology Data Exchange (ETDEWEB)

    Ganesan, Shanthi, E-mail: shanthig@iastate.edu; Keating, Aileen F., E-mail: akeating@iastate.edu

    2014-01-15

    7,12-Dimethylbenz[a]anthracene (DMBA) destroys ovarian follicles in a concentration-dependent manner. The impact of DMBA on connexin (CX) proteins that mediate communication between follicular cell types along with pro-apoptotic factors p53 and Bax were investigated. Postnatal day (PND) 4 Fisher 344 rat ovaries were cultured for 4 days in vehicle medium (1% DMSO) followed by a single exposure to vehicle control (1% DMSO) or DMBA (12.5 nM or 75 nM) and cultured for 4 or 8 days. RT-PCR was performed to quantify Cx37, Cx43, p53 and Bax mRNA level. Western blotting and immunofluorescence staining were performed to determine CX37 or CX43 level and/or localization. Cx37 mRNA and protein increased (P < 0.05) at 4 days of 12.5 nM DMBA exposure. Relative to vehicle control-treated ovaries, mRNA encoding Cx43 decreased (P < 0.05) but CX43 protein increased (P < 0.05) at 4 days by both DMBA exposures. mRNA expression of pro-apoptotic p53 was decreased (P < 0.05) but no changes in Bax expression were observed after 4 days of DMBA exposures. In contrast, after 8 days, DMBA decreased Cx37 and Cx43 mRNA and protein but increased both p53 and Bax mRNA levels. CX43 protein was located between granulosa cells, while CX37 was located at the oocyte cell surface of all follicle stages. These findings support that DMBA exposure impacts ovarian Cx37 and Cx43 mRNA and protein prior to both observed changes in pro-apoptotic p53 and Bax and follicle loss. It is possible that such interference in follicular cell communication is detrimental to follicle viability, and may play a role in DMBA-induced follicular atresia. - Highlights: • DMBA increases Cx37 and Cx43 expression prior to follicle loss. • During follicle loss both Cx37 and Cx43 expressions are reduced. • CX43 protein is absent in follicle remnants lacking an oocyte.

  9. Could tight junctions regulate the barrier function of the aged skin?

    Science.gov (United States)

    Svoboda, Marek; Bílková, Zuzana; Muthný, Tomáš

    2016-03-01

    The skin is known to be the largest organ in human organism creating interface with outer environment. The skin provides protective barrier against pathogens, physical and chemical insults, and against uncontrolled loss of water. The barrier function was primarily attributed to the stratum corneum (SC) but recent studies confirmed that epidermal tight junctions (TJs) also play important role in maintaining barrier properties of the skin. Independent observations indicate that barrier function and its recovery is impaired in aged skin. However, trans-epidermal water loss (TEWL) values remains rather unchanged in elderly population. UV radiation as major factor of photoageing impairs TJ proteins, but TJs have great self-regenerative potential. Since it may be possible that TJs can compensate TEWL in elderly due to its regenerative and compensatory capabilities, important question remains to be answered: how are TJs regulated during skin ageing? This review provides an insight into TJs functioning as epidermal barrier and summarizes current knowledge about the impact of ageing on the barrier function of the skin and epidermal TJs. Copyright © 2015 Elsevier Ireland Ltd. All rights reserved.

  10. Experimental Electron Heat Diffusion in TJ-II ECRH Plasmas

    Energy Technology Data Exchange (ETDEWEB)

    Vargas, V.I.; Lopez-Bruna, D.; Herranz, J.; Castejon, F.

    2006-07-01

    Interpretative transport has been used to revisit the global scalings of TJ-II ECRH plasmas from a local perspective. Density, rotational transform and ERCH power scans were analysed based upon Thomson Scattering data (electron density and temperature) in steady state discharges. A simple formula to obtain the thermal conductivity, assuming pure diffusion and negligible convective heat fluxes was used in a set of 161 discharges. All the analysis was performed with the ASTRA transport shell. The density scan indicates that inside n=0,4 there is no significant change of e with density in the range studied (0.4 (1019m-3) 1.0), while in 0,5 <0,8 approximately, e decreases with density. In the rotational transform scan it is found that the values of e when a low order rational of the rotational transform is present locally seem to be smaller for the corresponding range, although it is apparent a general beneficial effect of the corresponding change in magnetic structure. Finally, in the ECRH power scan, e is found to have an overall increment in 0,2

  11. Transient Particle Transport Analysis on TJ-II Stellarator

    Energy Technology Data Exchange (ETDEWEB)

    Eguilior, S.; Castejon, F.; Guasp, J.; Estrada, T.; Medina, F.; Tabares, F.L.; Branas, B.

    2006-12-18

    Particle diffusivity and convective velocity have been determined in ECRH plasmas confined in the stellarator TJ-II by analysing the evolving density profile. This is obtained from an amplitude modulation reflectometry system in addition to an X-ray tomographic reconstruction. The source term, which is needed as an input for transport equations, is obtained using EIRENE code. In order to discriminate between the diffusive and convective contributions, the dynamics of the density evolution has been analysed in several perturbative experiments. This evolution has been considered in discharges with injection of a single pulse of H2 as well as in those that present a spontaneous transition to an enhanced confinement mode and whose confinement properties are modified by inducing an ohmic current. The pinch velocity and diffusivity are parameterized by different expressions in order to fit the experimental time evolution of density profile. The profile evolution is very different from one case to another due to the different values of convective velocities and diffusivities, besides the different source terms. (Author) 19 refs.

  12. Particle Transport in ECRH Plasmas of the TJ-II

    International Nuclear Information System (INIS)

    Vargas, V. I.; Lopez-Bruna, D.; Estrada, T.; Guasp, J.; Reynolds, J. M.; Velasco, J. L.; Herranz, J.

    2007-01-01

    We present a systematic study of particle transport in ECRH plasmas of TJ-II with different densities. The goal is to fi nd particle confinement time and electron diffusivity dependence with line-averaged density. The experimental information consists of electron temperature profiles, T e (Thomson Scattering TS) and electron density, n e , (TS and reflectometry) and measured puffing data in stationary discharges. The profile of the electron source, Se, was obtained by the 3D Monte-Carlo code EIRENE. The analysis of particle balance has been done by linking the results of the code EIRENE with the results of a model that reproduces ECRH plasmas in stationary conditions. In the range of densities studied (0.58 ≤n e > (10 1 9m - 3) ≤0.80) there are two regions of confinement separated by a threshold density, e > ∼0.65 10 1 9m - 3. Below this threshold density the particle confinement time is low, and vice versa. This is reflected in the effective diffusivity, D e , which in the range of validity of this study, 0.5 e are flat for ≥0,63(10 1 9m - 3). (Author) 35 refs

  13. Experimental Electron Heat Diffusion in TJ-II ECRH Plasmas

    International Nuclear Information System (INIS)

    Vargas, V.I.; Lopez-Bruna, D.; Herranz, J.; Castejon, F.

    2006-01-01

    Interpretative transport has been used to revisit the global scalings of TJ-II ECRH plasmas from a local perspective. Density, rotational transform and ERCH power scans were analysed based upon Thomson Scattering data (electron density and temperature) in steady state discharges. A simple formula to obtain the thermal conductivity, assuming pure diffusion and negligible convective heat fluxes was used in a set of 161 discharges. All the analysis was performed with the ASTRA transport shell. The density scan indicates that inside n=0,4 there is no significant change of e with density in the range studied (0.4 (1019m-3) 1.0), while in 0,5 <0,8 approximately, e decreases with density. In the rotational transform scan it is found that the values of e when a low order rational of the rotational transform is present locally seem to be smaller for the corresponding range, although it is apparent a general beneficial effect of the corresponding change in magnetic structure. Finally, in the ECRH power scan, e is found to have an overall increment in 0,2< n0,6 when QECH increases from 200 to 400 kW, although it is less significant in the density gradient region (n 0,7). (Author) 22 refs

  14. NBI Calculations for the TJ-II Experimental discharges

    International Nuclear Information System (INIS)

    Guasp, J.; Fuentes, C.; Liniers, M.

    2004-01-01

    Calculations for NBI losses, absorption and power deposition radial profiles, corresponding to the experimental TJ-II campaigns 2003-2004, have been fitted to simple functionals in order to allow a fast approximative evaluation for any given density. The average difference between the calculations for the individual discharges using the experimental density and temperature radial profiles and the fit predictions are between 10 and 15% and the behaviour with density is the expected one: nonotonic decrease of shine through losses and increase of absorption with incipient saturation for high densities. The fast ion birth radial profile narrows initially at low densities but later starts to widen, although, for the average line density range analysed (0.51 a 4.1x10''13 cm''-3), never are wide enough to induce an increase of direct orbit losses neither to produce hollow radial profiles. The power absorption radial profile widens nonotonically. There exist Fortran subroutines, available at the three CIEMAT computers, allowing the fast approximative evaluation of all these values. (Author) 8 refs

  15. NBI Calculations for the TJ-II Experimental Discharges

    International Nuclear Information System (INIS)

    Guasp, J.; Fuentes, C.; Liniers, M.

    2005-01-01

    The density and electron temperature radial profiles, corresponding to the experimental TJ-II campaigns 2003-2004, with NBI, have been fitted to simple functionals in order to allow a fast approximative evaluation for any given density and injected power... The fits have been calculated, separately, for the four possibilities: ECRH and NBI Phases as well as On and Off Axis ECRH injection. The average difference between the experimental profiles for the individual discharges and the fit predictions are around 8% for the density and 10% for the temperature. The behaviour of the predicted profiles with average line density and injected power has been analysed. The central electron temperature decreases monotonically with increasing density and the ECRH phase On Axis central value is clearly higher than the Off axis one. The radial density profiles narrow with increasing density and the NBI On axis case is clearly wider than de Off one. The electron temperature profile widens slightly with increasing density and the width of the On Axix case is lesser than for the Off case in all phases. There exist Fortran subroutines, available at the three CIEMAT computers, allowing the fast approximative evaluation of all these profiles. (Author) 8 refs

  16. A novel biosorbent for dye removal: Extracellular polymeric substance (EPS) of Proteus mirabilis TJ-1

    Energy Technology Data Exchange (ETDEWEB)

    Zhang Zhiqiang [State Key Laboratory of Pollution Control and Resource Reuse, Key Laboratory of Yangtze River Water Environment of Ministry of Education, College of Environmental Science and Engineering, Tongji University, Shanghai 200092 (China); Laboratoire de Sciences Analytiques (UMR CNRS 5180), Universite Claude Bernard Lyon 1, Universite de Lyon, 69622 Villeurbanne Cedex (France); Xia Siqing [State Key Laboratory of Pollution Control and Resource Reuse, Key Laboratory of Yangtze River Water Environment of Ministry of Education, College of Environmental Science and Engineering, Tongji University, Shanghai 200092 (China)], E-mail: siqingxia@mail.tongji.edu.cn; Wang Xuejiang; Yang Aming; Xu Bin; Chen Ling; Zhu Zhiliang; Zhao Jianfu [State Key Laboratory of Pollution Control and Resource Reuse, Key Laboratory of Yangtze River Water Environment of Ministry of Education, College of Environmental Science and Engineering, Tongji University, Shanghai 200092 (China); Jaffrezic-Renault, Nicole; Leonard, Didier [Laboratoire de Sciences Analytiques (UMR CNRS 5180), Universite Claude Bernard Lyon 1, Universite de Lyon, 69622 Villeurbanne Cedex (France)

    2009-04-15

    This paper deals with the extracellular polymeric substance (EPS) of Proteus mirabilis TJ-1 used as a novel biosorbent to remove dye from aqueous solution in batch systems. As a widely used and hazardous dye, basic blue 54 (BB54) was chosen as the model dye to examine the adsorption performance of the EPS. The effects of pH, initial dye concentration, contact time and temperature on the sorption of BB54 to the EPS were examined. At various initial dye concentrations (50-400 mg/L), the batch sorption equilibrium can be obtained in only 5 min. Kinetic studies suggested that the sorption followed the internal transport mechanism. According to the Langmuir model, the maximum BB54 uptake of 2.005 g/g was obtained. Chemical analysis of the EPS indicated the presence of protein (30.9%, w/w) and acid polysaccharide (63.1%, w/w). Scanning electron microscopy (SEM) images showed that the EPS with a crystal-linear structure was whole enwrapped by adsorbed dye molecules. FTIR spectrum result revealed the presence of adsorbing groups such as carboxyl, hydroxyl and amino groups in the EPS. High-molecular weight of the EPS with more binding-sites and stronger van der Waals forces together with its specific construct leads to the excellent performance of dye adsorption. The EPS shows potential board application as a biosorbent for both environmental protection and dye recovery.

  17. A novel biosorbent for dye removal: Extracellular polymeric substance (EPS) of Proteus mirabilis TJ-1

    International Nuclear Information System (INIS)

    Zhang Zhiqiang; Xia Siqing; Wang Xuejiang; Yang Aming; Xu Bin; Chen Ling; Zhu Zhiliang; Zhao Jianfu; Jaffrezic-Renault, Nicole; Leonard, Didier

    2009-01-01

    This paper deals with the extracellular polymeric substance (EPS) of Proteus mirabilis TJ-1 used as a novel biosorbent to remove dye from aqueous solution in batch systems. As a widely used and hazardous dye, basic blue 54 (BB54) was chosen as the model dye to examine the adsorption performance of the EPS. The effects of pH, initial dye concentration, contact time and temperature on the sorption of BB54 to the EPS were examined. At various initial dye concentrations (50-400 mg/L), the batch sorption equilibrium can be obtained in only 5 min. Kinetic studies suggested that the sorption followed the internal transport mechanism. According to the Langmuir model, the maximum BB54 uptake of 2.005 g/g was obtained. Chemical analysis of the EPS indicated the presence of protein (30.9%, w/w) and acid polysaccharide (63.1%, w/w). Scanning electron microscopy (SEM) images showed that the EPS with a crystal-linear structure was whole enwrapped by adsorbed dye molecules. FTIR spectrum result revealed the presence of adsorbing groups such as carboxyl, hydroxyl and amino groups in the EPS. High-molecular weight of the EPS with more binding-sites and stronger van der Waals forces together with its specific construct leads to the excellent performance of dye adsorption. The EPS shows potential board application as a biosorbent for both environmental protection and dye recovery

  18. Inhibition of gap-junctional intercellular communication and activation of mitogen-activated protein kinases by cyanobacterial extracts--indications of novel tumor-promoting cyanotoxins?

    Science.gov (United States)

    Bláha, Ludĕk; Babica, Pavel; Hilscherová, Klára; Upham, Brad L

    2010-01-01

    Toxicity and liver tumor promotion of cyanotoxins microcystins have been extensively studied. However, recent studies document that other metabolites present in the complex cyanobacterial water blooms may also have adverse health effects. In this study we used rat liver epithelial stem-like cells (WB-F344) to examine the effects of cyanobacterial extracts on two established markers of tumor promotion, inhibition of gap-junctional intercellular communication (GJIC) and activation of mitogen-activated protein kinases (MAPKs) - ERK1/2. Extracts of cyanobacteria (laboratory cultures of Microcystis aeruginosa and Aphanizomenon flos-aquae and water blooms dominated by these species) inhibited GJIC and activated MAPKs in a dose-dependent manner (effective concentrations ranging 0.5-5mgd.w./mL). Effects were independent of the microcystin content and the strongest responses were elicited by the extracts of Aphanizomenon sp. Neither pure microcystin-LR nor cylindrospermopsin inhibited GJIC or activated MAPKs. Modulations of GJIC and MAPKs appeared to be specific to cyanobacterial extracts since extracts from green alga Chlamydomonas reinhardtii, heterotrophic bacterium Klebsiella terrigena, and isolated bacterial lipopolysaccharides had no comparable effects. Our study provides the first evidence on the existence of unknown cyanobacterial toxic metabolites that affect in vitro biomarkers of tumor promotion, i.e. inhibition of GJIC and activation of MAPKs.

  19. Neuropeptide Y, substance P, and human bone morphogenetic protein 2 stimulate human osteoblast osteogenic activity by enhancing gap junction intercellular communication

    International Nuclear Information System (INIS)

    Ma, W.H.; Liu, Y.J.; Wang, W.; Zhang, Y.Z.

    2015-01-01

    Bone homeostasis seems to be controlled by delicate and subtle “cross talk” between the nervous system and “osteo-neuromediators” that control bone remodeling. The purpose of this study was to evaluate the effect of interactions between neuropeptides and human bone morphogenetic protein 2 (hBMP2) on human osteoblasts. We also investigated the effects of neuropeptides and hBMP2 on gap junction intercellular communication (GJIC). Osteoblasts were treated with neuropeptide Y (NPY), substance P (SP), or hBMP2 at three concentrations. At various intervals after treatment, cell viability was measured by the MTT assay. In addition, cellular alkaline phosphatase (ALP) activity and osteocalcin were determined by colorimetric assay and radioimmunoassay, respectively. The effects of NPY, SP and hBMP on GJIC were determined by laser scanning confocal microscopy. The viability of cells treated with neuropeptides and hBMP2 increased significantly in a time-dependent manner, but was inversely associated with the concentration of the treatments. ALP activity and osteocalcin were both reduced in osteoblasts exposed to the combination of neuropeptides and hBMP2. The GJIC of osteoblasts was significantly increased by the neuropeptides and hBMP2. These results suggest that osteoblast activity is increased by neuropeptides and hBMP2 through increased GJIC. Identification of the GJIC-mediated signal transduction capable of modulating the cellular activities of bone cells represents a novel approach to studying the biology of skeletal innervation

  20. Neuropeptide Y, substance P, and human bone morphogenetic protein 2 stimulate human osteoblast osteogenic activity by enhancing gap junction intercellular communication

    Energy Technology Data Exchange (ETDEWEB)

    Ma, W.H.; Liu, Y.J.; Wang, W.; Zhang, Y.Z. [The Third Hospital of Hebei Medical University, The Provincial Key Laboratory for Orthopedic Biomechanics of Hebei, Shijiazhuang, Hebei Province (China)

    2015-02-13

    Bone homeostasis seems to be controlled by delicate and subtle “cross talk” between the nervous system and “osteo-neuromediators” that control bone remodeling. The purpose of this study was to evaluate the effect of interactions between neuropeptides and human bone morphogenetic protein 2 (hBMP2) on human osteoblasts. We also investigated the effects of neuropeptides and hBMP2 on gap junction intercellular communication (GJIC). Osteoblasts were treated with neuropeptide Y (NPY), substance P (SP), or hBMP2 at three concentrations. At various intervals after treatment, cell viability was measured by the MTT assay. In addition, cellular alkaline phosphatase (ALP) activity and osteocalcin were determined by colorimetric assay and radioimmunoassay, respectively. The effects of NPY, SP and hBMP on GJIC were determined by laser scanning confocal microscopy. The viability of cells treated with neuropeptides and hBMP2 increased significantly in a time-dependent manner, but was inversely associated with the concentration of the treatments. ALP activity and osteocalcin were both reduced in osteoblasts exposed to the combination of neuropeptides and hBMP2. The GJIC of osteoblasts was significantly increased by the neuropeptides and hBMP2. These results suggest that osteoblast activity is increased by neuropeptides and hBMP2 through increased GJIC. Identification of the GJIC-mediated signal transduction capable of modulating the cellular activities of bone cells represents a novel approach to studying the biology of skeletal innervation.

  1. Influence of Th2 Cytokines on the Cornified Envelope, Tight Junction Proteins, and ß-Defensins in Filaggrin-Deficient Skin Equivalents.

    Science.gov (United States)

    Hönzke, Stefan; Wallmeyer, Leonie; Ostrowski, Anja; Radbruch, Moritz; Mundhenk, Lars; Schäfer-Korting, Monika; Hedtrich, Sarah

    2016-03-01

    Atopic dermatitis is a chronic skin condition with complex etiology. It is characterized by skin barrier defects and T helper type 2 (Th2)-polarized inflammation. Although mutations in the filaggrin gene are known to be prominent genetic risk factors for the development of atopic dermatitis, the interdependency between these and an altered cytokine milieu is not fully understood. In this study, we evaluated the direct effects of filaggrin deficiency on the cornified envelope, tight junction proteins, and innate immune response, and report the effects of Th2 cytokines in normal and filaggrin-deficient skin equivalents. Supplementation with IL-4 and IL-13 led to distinct histologic changes and significantly increased skin surface pH, both of which were enhanced in filaggrin knockdown skin equivalents. We detected a compensatory up-regulation of involucrin and occludin in filaggrin-deficient skin that was dramatically disturbed when simultaneous inflammation occurred. Furthermore, we found that a lack of filaggrin triggered an up-regulation of human ?-defensin 2 via an unknown mechanism, which was abolished by Th2 cytokine supplementation. Taken together, these results indicate that defects in the epidermal barrier, skin permeability, and cutaneous innate immune response are not primarily linked to filaggrin deficiency but are rather secondarily induced by Th2 inflammation. Copyright © 2015 The Authors. Published by Elsevier Inc. All rights reserved.

  2. Effect of Chum Salmon Egg Lectin on Tight Junctions in Caco-2 Cell Monolayers

    Directory of Open Access Journals (Sweden)

    Ryo Nemoto

    2015-05-01

    Full Text Available The effect of a chum salmon egg lectin (CSL3 on tight junction (TJ of Caco-2 cell monolayers was investigated. The lectin opened TJ as indicated by the decrease of the transepithelial electrical resistance (TER value and the increase of the permeation of lucifer yellow, which is transported via the TJ-mediated paracellular pathway. The effects of CSL3 were inhibited by the addition of 10 mM L-rhamnose or D-galactose which were specific sugars for CSL3. The lectin increased the intracellular Ca2+ of Caco-2 cell monolayers, that could be inhibited by the addition of L-rhamnose. The fluorescence immunostaining of β-actin in Caco-2 cell monolayers revealed that the cytoskeleton was changed by the CSL3 treatment, suggesting that CSL3 depolymerized β-actin to cause reversible TJ structural and functional disruption. Although Japanese jack bean lectin and wheat germ lectin showed similar effects in the decrease of the TER values and the increase of the intracellular Ca2+, they could not be inhibited by the same concentrations of simple sugars, such as D-glucose and N-acetyl-D-glucosamine.

  3. E. coli O124 K72 alters the intestinal barrier and the tight junctions proteins of guinea pig intestine.

    Science.gov (United States)

    Ren, Xiaomeng; Zhu, Yanyan; Gamallat, Yaser; Ma, Shenhao; Chiwala, Gift; Meyiah, Abdo; Xin, Yi

    2017-10-01

    Our research group previously isolated and identified a strain of pathogenic Escherichia coli from clinical samples called E. coli O124 K72. The present study was aimed at determining the potential effects of E. coli O124 K72 on intestinal barrier functions and structural proteins integrity in guinea pig. Guinea pigs were grouped into three groups; control (CG); E. coli O124 K72 (E. coli); and probiotics Lactobacillus rhamnosus (LGG). Initially, we create intestinal dysbiosis by giving all animals Levofloxacin for 10days, but the control group (CG) received the same volume of saline. Then, the animals received either E. coli O124 K72 (E. coli) or Lactobacillus rhamnosus (LGG) according to their assigned group. E. coli O124 K72 treatment significantly affected colon morphology and distorted intestinal barrier function by up-regulating Claudin2 and down-regulating Occludin. In addition, E. coli upregulated the mRNA expression of MUC1, MUC2, MUC13 and MUC15. Furthermore, suspected tumor was found in the E. coli treated animals. Our results suggested that E. coli O124 K72 strain has adverse effects on intestinal barrier functions and is capable of altering integrity of structural proteins in guinea pig model while at same time it may have a role in colon carcinogenesis. Copyright © 2017 Elsevier Masson SAS. All rights reserved.

  4. Microcomputer based system to control the load of a capacitor array in the TJ-1 Tokamak; Sistema de control de carga de condensadores del TJ-1

    Energy Technology Data Exchange (ETDEWEB)

    Alberdi, J.; Asenjo, L.; Sanz, J.A.

    1990-12-31

    The power to create the magnetic field in the TJ-1 TOKAMAK is provide by an array of 16 capacitor sets. The total capacity of this array is 8.1F. This work describes a computer system based on the Motorola M-6800 microprocessor which controls the load of the capacitor set-and stablishes the conditions for the reactor trigger. (author)

  5. Microcomputer based system to control the load of a capacitor array in the TJ-1 Tokamak. Sistema de control de carga de condensadores del TJ-1

    Energy Technology Data Exchange (ETDEWEB)

    Alberdi, J.; Asenjo, L.; Sanz, J.A.

    1990-01-01

    The power to create the magnetic field in the TJ-1 TOKAMAK is provide by an array of 16 capacitor sets. The total capacity of this array is 8.1F. This work describes a computer system based on the Motorola M-6800 microprocessor which controls the load of the capacitor set-and stablishes the conditions for the reactor trigger. (author)

  6. Microcomputer Based System to control the Load of a Capacitor Array in the TJ-1 Tokamak; Sistema de Control de Carga de Condensadores del TJ-1

    Energy Technology Data Exchange (ETDEWEB)

    Alberdi, J; Asenso, L; Sanz, J A

    1990-07-01

    The power to create the magnetic fields in the TJ-1 Tokamak is provides by an array of 16 capacitor sets. The total capacity of this array is 8. 1F. This work describes a computer system based on the Motorola M-6800 micro- processor which controls the load of the capacitor set and stablished the conditions for the reactor trigger. (Author)

  7. Particle Transport in ECRH Plasmas of the TJ-II; Transporte de Particulas en Plasmas ECRH del TJ-II

    Energy Technology Data Exchange (ETDEWEB)

    Vargas, V. I.; Lopez-Bruna, D.; Estrada, T.; Guasp, J.; Reynolds, J. M.; Velasco, J. L.; Herranz, J.

    2007-07-01

    We present a systematic study of particle transport in ECRH plasmas of TJ-II with different densities. The goal is to fi nd particle confinement time and electron diffusivity dependence with line-averaged density. The experimental information consists of electron temperature profiles, T{sub e} (Thomson Scattering TS) and electron density, n{sub e}, (TS and reflectometry) and measured puffing data in stationary discharges. The profile of the electron source, Se, was obtained by the 3D Monte-Carlo code EIRENE. The analysis of particle balance has been done by linking the results of the code EIRENE with the results of a model that reproduces ECRH plasmas in stationary conditions. In the range of densities studied (0.58 {<=}n{sub e}> (10{sup 1}9m{sup -}3) {<=}0.80) there are two regions of confinement separated by a threshold density, {approx}0.65 10{sup 1}9m{sup -}3. Below this threshold density the particle confinement time is low, and vice versa. This is reflected in the effective diffusivity, D{sub e}, which in the range of validity of this study, 0.5 <{rho}<0.9 being {rho} normalized plasma radius, decreased significantly above the threshold density. The profiles of D{sub e} are flat for {>=}0,63(10{sup 1}9m{sup -}3). (Author) 35 refs.

  8. You Shall Not Pass – Tight junctions of the Blood Brain Barrier.

    Directory of Open Access Journals (Sweden)

    Hans-Christian eBauer

    2014-12-01

    Full Text Available Tissue barriers restricting the free diffusion of substances between the central nervous system and the systemic circulation are of great medical interest. Excessive leakage of blood-borne molecules into the parenchyma and the concomitant fluctuations in the microenvironment following a transient breakdown of the blood-brain barrier (BBB during ischemic/hypoxic conditions or due to an autoimmune disease are detrimental to the physiology of nervous tissue.On the other hand, the treatment of neurological disorders is often hampered as only minimal amounts of therapeutic agents are able to penetrate a functional BBB or blood cerebrospinal fluid barrier. At the basis of the BBB are, next to an elaborate transporting machinery, intimate cell-cell contacts (tight junctions creating not only a paracellular diffusion constraint but also enabling the vectorial transport across cell monolayers.More recent findings indicate that functional barriers are already established during development, protecting the fetal brain. As an understanding of the biogenesis of TJs might reveal the underlying mechanisms of barrier formation during ontogenic development numerous in vitro systems have been developed to study the assembly and disassembly of TJs. In addition, monitoring the stage-specific expression of TJ proteins during development has brought much insight into the developmental tightening of tissue barriers. Further, over the last two decades a detailed molecular map of tight junctions has emerged.TJs not only represent a cell-cell adhesion structure, but integrate various signaling pathways, thereby directly or indirectly impacting upon processes such as cell proliferation, cytoskeletal rearrangement, and transcriptional control.This review will provide a brief overview on the establishment of the BBB during embryonic development in mammals and a detailed description of the ultrastructure, biogenesis, and molecular composition of epithelial and endothelial

  9. Proximal tubule proteins are significantly elevated in bladder urine of patients with ureteropelvic junction obstruction and may represent novel biomarkers: A pilot study.

    Science.gov (United States)

    Gerber, Claire; Harel, Miriam; Lynch, Miranda L; Herbst, Katherine W; Ferrer, Fernando A; Shapiro, Linda H

    2016-04-01

    Ureteropelvic junction obstruction (UPJO) is the major cause of hydronephrosis in children and may lead to renal injury and early renal dysfunction. However, diagnosis of the degree of obstruction and severity of renal injury relies on invasive and often inconclusive renal scans. Biomarkers from voided urine that detect early renal injury are highly desirable because of their noninvasive collection and their potential to assist in earlier and more reliable diagnosis of the severity of obstruction. Early in response to UPJO, increased intrarenal pressure directly impacts the proximal tubule brush border. We hypothesize that single-pass, apically expressed proximal tubule brush border proteins will be shed into the urine early and rapidly and will be reliable noninvasive urinary biomarkers, providing the tools for a more reliable stratification of UPJO patients. We performed a prospective cohort study at Connecticut Children's Medical Center. Bladder urine samples from 12 UPJO patients were obtained prior to surgical intervention. Control urine samples were collected from healthy pediatric patients presenting with primary nocturnal enuresis. We determined levels of NGAL, KIM-1 (previously identified biomarkers), CD10, CD13, and CD26 (potentially novel biomarkers) by ELISA in control and experimental urine samples. Urinary creatinine levels were used to normalize the urinary protein levels measured by ELISA. Each of the proximal tubule proteins outperformed the previously published biomarkers. No differences in urinary NGAL and KIM-1 levels were observed between control and obstructed patients (p = 0.932 and p = 0.799, respectively). However, levels of CD10, CD13, and CD26 were significantly higher in the voided urine of obstructed individuals when compared with controls (p = 0.002, p = 0.024, and p = 0.007, respectively) (Figure). Targeted identification of reliable, noninvasive biomarkers of renal injury is critical to aid in diagnosing patients at risk, guiding

  10. Carbachol ameliorates lipopolysaccharide-induced intestinal epithelial tight junction damage by down-regulating NF-{kappa}{beta} and myosin light-chain kinase pathways

    Energy Technology Data Exchange (ETDEWEB)

    Zhang, Ying [Department of Anesthesia, Critical Care Medicine and Emergency Medicine Center, Zhongnan Hospital, Wuhan University, Wuhan 430071, Hubei Province, People' s Republic of China (China); Li, Jianguo, E-mail: 2010lijianguo@sina.cn [Department of Anesthesia, Critical Care Medicine and Emergency Medicine Center, Zhongnan Hospital, Wuhan University, Wuhan 430071, Hubei Province, People' s Republic of China (China)

    2012-11-16

    Highlights: Black-Right-Pointing-Pointer Carbachol reduced the lipopolysaccharide-induced intestinal barrier breakdown. Black-Right-Pointing-Pointer Carbachol ameliorated the lipopolysaccharide-induced ileal tight junction damage. Black-Right-Pointing-Pointer Carbachol prevented the LPS-induced NF-{kappa}{beta} and myosin light-chain kinase activation. Black-Right-Pointing-Pointer Carbachol exerted its beneficial effects in an {alpha}7 nicotinic receptor-dependent manner. -- Abstract: Carbachol is a cholinergic agonist that protects the intestines after trauma or burn injury. The present study determines the beneficial effects of carbachol and the mechanisms by which it ameliorates the lipopolysaccharide (LPS)-induced intestinal barrier breakdown. Rats were injected intraperitoneally with 10 mg/kg LPS. Results showed that the gut barrier permeability was reduced, the ultrastructural disruption of tight junctions (TJs) was prevented, the redistribution of zonula occludens-1 and claudin-2 proteins was partially reversed, and the nuclear factor-kappa beta (NF-{kappa}{beta}) and myosin light-chain kinase (MLCK) activation in the intestinal epithelium were suppressed after carbachol administration in LPS-exposed rats. Pretreatment with the {alpha}7 nicotinic acetylcholine receptor ({alpha}7nAchR) antagonist {alpha}-bungarotoxin blocked the protective action of carbachol. These results suggested that carbachol treatment can protect LPS-induced intestinal barrier dysfunction. Carbachol exerts its beneficial effect on the amelioration of the TJ damage by inhibiting the NF-{kappa}{beta} and MLCK pathways in an {alpha}7nAchR-dependent manner.

  11. Hypoxic Stress and Inflammatory Pain Disrupt Blood-Brain Barrier Tight Junctions: Implications for Drug Delivery to the Central Nervous System.

    Science.gov (United States)

    Lochhead, Jeffrey J; Ronaldson, Patrick T; Davis, Thomas P

    2017-07-01

    A functional blood-brain barrier (BBB) is necessary to maintain central nervous system (CNS) homeostasis. Many diseases affecting the CNS, however, alter the functional integrity of the BBB. It has been shown that various diseases and physiological stressors can impact the BBB's ability to selectively restrict passage of substances from the blood to the brain. Modifications of the BBB's permeability properties can potentially contribute to the pathophysiology of CNS diseases and result in altered brain delivery of therapeutic agents. Hypoxia and/or inflammation are central components of a number of diseases affecting the CNS. A number of studies indicate hypoxia or inflammatory pain increase BBB paracellular permeability, induce changes in the expression and/or localization of tight junction proteins, and affect CNS drug uptake. In this review, we look at what is currently known with regard to BBB disruption following a hypoxic or inflammatory insult in vivo. Potential mechanisms involved in altering tight junction components at the BBB are also discussed. A more detailed understanding of the mediators involved in changing BBB functional integrity in response to hypoxia or inflammatory pain could potentially lead to new treatments for CNS diseases with hypoxic or inflammatory components. Additionally, greater insight into the mechanisms involved in TJ rearrangement at the BBB may lead to novel strategies to pharmacologically increase delivery of drugs to the CNS.

  12. Resonant Effects in Neutral beam Moderation at TJ-II

    International Nuclear Information System (INIS)

    Guasp, J.; Liniers, M.; Fuentes, C.

    1999-01-01

    The structure of fast ion losses in neutral beam moderation at TJ-II is analysed theoretically, in particular the influence of resonant effects and the radial electric field dependence. The direct losses show strong resonant effects when the ratio of the poloidal and toroidal rotation velocities pass near the values -4/3, -2 or 0. These effects are visible as strong maxima on the loss fractions and also as characteristic trajectory behaviours. The delayed losses present resonant effects also, generally at intermediate energies (5 to 20 KeV for 40 keV injection). Near the resonances the population of passing particles in these losses is very high and the loss fraction can equal or even surpass the direct losses. In these delayed losses the particles concentrate along vertical strips on the loss cone diagrams of roughly constant parallel velocity. This parallel velocity increases with the electric field, the loss maxima are reached usually when the pitch and energy of these strips are near the initial injection values. The trapped particle population in these delayed losses is maximal at null electric and decreases with the field intensity for both potential signs. The corresponding final energies are usually low (1 to 5 keV). Except at extreme potentials, where the -4 resonance can appear, no resonant effects are observed for this population. The resonance ordering is similar for all configurations and follows reasonably the predictions of a simple model. The extreme configurations are dominated by the effects of the 0 and -2 resonances, giving very high loss fractions even for null electric field. In contrast the intermediate configurations, near the Reference one, do not present resonant effects and the losses are moderate at low potentials. Only above 2000 v the resonant effects start to appear. (Author) 11 refs

  13. Molecular cloning, functional expression, and tissue distribution of a novel human gap junction-forming protein, connexin-31.9. Interaction with zona occludens protein-1

    NARCIS (Netherlands)

    Nielsen, Peter A; Beahm, Derek L; Giepmans, Ben N G; Baruch, Amos; Hall, James E; Kumar, Nalin M

    2002-01-01

    A novel human connexin gene (GJA11) was cloned from a genomic library. The open reading frame encoded a hypothetical protein of 294 amino acid residues with a predicted molecular mass of 31,933, hence referred to as connexin-31.9 (Cx31.9) or alpha 11 connexin. A clone in GenBank containing the

  14. Data management in the TJ-II multi-layer database

    International Nuclear Information System (INIS)

    Vega, J.; Cremy, C.; Sanchez, E.; Portas, A.; Fabregas, J.A.; Herrera, R.

    2000-01-01

    The handling of TJ-II experimental data is performed by means of several software modules. These modules provide the resources for data capture, data storage and management, data access as well as general-purpose data visualisation. Here we describe the module related to data storage and management. We begin by introducing the categories in which data can be classified. Then, we describe the TJ-II data flow through the several file systems involved, before discussing the architecture of the TJ-II database. We review the concept of the 'discharge file' and identify the drawbacks that would result from a direct application of this idea to the TJ-II data. In order to overcome these drawbacks, we propose alternatives based on our concepts of signal family, user work-group and data priority. Finally, we present a model for signal storage. This model is in accordance with the database architecture and provides a proper framework for managing the TJ-II experimental data. In the model, the information is organised in layers and is distributed according to the generality of the information, from the common fields of all signals (first layer), passing through the specific records of signal families (second layer) and reaching the particular information of individual signals (third layer)

  15. Membrane Receptor-Induced Changes of the Protein Kinases A and C Activity May Play a Leading Role in Promoting Developmental Synapse Elimination at the Neuromuscular Junction.

    Science.gov (United States)

    Tomàs, Josep M; Garcia, Neus; Lanuza, Maria A; Nadal, Laura; Tomàs, Marta; Hurtado, Erica; Simó, Anna; Cilleros, Víctor

    2017-01-01

    Synapses that are overproduced during histogenesis in the nervous system are eventually lost and connectivity is refined. Membrane receptor signaling leads to activity-dependent mutual influence and competition between axons directly or with the involvement of the postsynaptic cell and the associated glial cell/s. Presynaptic muscarinic acetylcholine (ACh) receptors (subtypes mAChR; M 1 , M 2 and M 4 ), adenosine receptors (AR; A 1 and A 2A ) and the tropomyosin-related kinase B receptor (TrkB), among others, all cooperate in synapse elimination. Between these receptors there are several synergistic, antagonic and modulatory relations that clearly affect synapse elimination. Metabotropic receptors converge in a limited repertoire of intracellular effector kinases, particularly serine protein kinases A and C (PKA and PKC), to phosphorylate protein targets and bring about structural and functional changes leading to axon loss. In most cells A 1 , M 1 and TrkB operate mainly by stimulating PKC whereas A 2A , M 2 and M 4 inhibit PKA. We hypothesize that a membrane receptor-induced shifting in the protein kinases A and C activity (inhibition of PKA and/or stimulation of PKC) in some nerve endings may play an important role in promoting developmental synapse elimination at the neuromuscular junction (NMJ). This hypothesis is supported by: (i) the tonic effect (shown by using selective inhibitors) of several membrane receptors that accelerates axon loss between postnatal days P5-P9; (ii) the synergistic, antagonic and modulatory effects (shown by paired inhibition) of the receptors on axonal loss; (iii) the fact that the coupling of these receptors activates/inhibits the intracellular serine kinases; and (iv) the increase of the PKA activity, the reduction of the PKC activity or, in most cases, both situations simultaneously that presumably occurs in all the situations of singly and paired inhibition of the mAChR, AR and TrkB receptors. The use of transgenic animals and

  16. Membrane Receptor-Induced Changes of the Protein Kinases A and C Activity May Play a Leading Role in Promoting Developmental Synapse Elimination at the Neuromuscular Junction

    Directory of Open Access Journals (Sweden)

    Josep M. Tomàs

    2017-08-01

    Full Text Available Synapses that are overproduced during histogenesis in the nervous system are eventually lost and connectivity is refined. Membrane receptor signaling leads to activity-dependent mutual influence and competition between axons directly or with the involvement of the postsynaptic cell and the associated glial cell/s. Presynaptic muscarinic acetylcholine (ACh receptors (subtypes mAChR; M1, M2 and M4, adenosine receptors (AR; A1 and A2A and the tropomyosin-related kinase B receptor (TrkB, among others, all cooperate in synapse elimination. Between these receptors there are several synergistic, antagonic and modulatory relations that clearly affect synapse elimination. Metabotropic receptors converge in a limited repertoire of intracellular effector kinases, particularly serine protein kinases A and C (PKA and PKC, to phosphorylate protein targets and bring about structural and functional changes leading to axon loss. In most cells A1, M1 and TrkB operate mainly by stimulating PKC whereas A2A, M2 and M4 inhibit PKA. We hypothesize that a membrane receptor-induced shifting in the protein kinases A and C activity (inhibition of PKA and/or stimulation of PKC in some nerve endings may play an important role in promoting developmental synapse elimination at the neuromuscular junction (NMJ. This hypothesis is supported by: (i the tonic effect (shown by using selective inhibitors of several membrane receptors that accelerates axon loss between postnatal days P5–P9; (ii the synergistic, antagonic and modulatory effects (shown by paired inhibition of the receptors on axonal loss; (iii the fact that the coupling of these receptors activates/inhibits the intracellular serine kinases; and (iv the increase of the PKA activity, the reduction of the PKC activity or, in most cases, both situations simultaneously that presumably occurs in all the situations of singly and paired inhibition of the mAChR, AR and TrkB receptors. The use of transgenic animals and various

  17. Baicalin Protects against TNF-α-Induced Injury by Down-Regulating miR-191a That Targets the Tight Junction Protein ZO-1 in IEC-6 Cells.

    Science.gov (United States)

    Wang, Li; Zhang, Ren; Chen, Jian; Wu, Qihui; Kuang, Zaoyuan

    2017-04-01

    Tumor necrosis factor-alpha (TNF-α) plays an important role in the developing process of inflammatory bowel disease. Tight junction protein zonula occludens-1 (ZO-1), one of epithelial junctional proteins, maintains the permeability of intestinal barrier. The objective of this study was to investigate the mechanism of the protective effect of baicalin on TNF-α-induced injury and ZO-1 expression in intestinal epithelial cells (IECs). We found that baicalin pretreatment significantly improved cell viability and cell migration following TNF-α stimulation. miR-191a inhibitor increased the protective effect of baicalin on cell motility injured by TNF-α. In addition, miR-191a down-regulated the mRNA and protein level of its target gene ZO-1. TNF-α stimulation increased miR-191a expression, leading to the decline of ZO-1 mRNA and protein. Moreover, pretreatment with baicalin reversed TNF-α induced decrease of ZO-1 and increase of miR-191a, miR-191a inhibitor significantly enhanced ZO-1 protein expression restored by baicalin. These results indicate that baicalin exerts a protective effect on IEC-6 (rat small intestinal epithelial cells) cells against TNF-α-induced injury, which is at least partly via inhibiting the expression of miR-191a, thus increasing ZO-1 mRNA and protein levels.

  18. GaN-based vertical-cavity surface-emitting lasers with tunnel junction contacts grown by metal-organic chemical vapor deposition

    Science.gov (United States)

    Lee, SeungGeun; Forman, Charles A.; Lee, Changmin; Kearns, Jared; Young, Erin C.; Leonard, John T.; Cohen, Daniel A.; Speck, James S.; Nakamura, Shuji; DenBaars, Steven P.

    2018-06-01

    We report the first demonstration of III–nitride vertical-cavity surface-emitting lasers (VCSELs) with tunnel junction (TJ) intracavity contacts grown completely by metal–organic chemical vapor deposition (MOCVD). For the TJs, n++-GaN was grown on in-situ activated p++-GaN after buffered HF surface treatment. The electrical properties and epitaxial morphologies of the TJs were first investigated on TJ LED test samples. A VCSEL with a TJ intracavity contact showed a lasing wavelength of 408 nm, a threshold current of ∼15 mA (10 kA/cm2), a threshold voltage of 7.8 V, a maximum output power of 319 µW, and a differential efficiency of 0.28%.

  19. Hybrid tunnel junction contacts to III–nitride light-emitting diodes

    KAUST Repository

    Young, Erin C.

    2016-01-26

    In this work, we demonstrate highly doped GaN p–n tunnel junction (TJ) contacts on III–nitride heterostructures where the active region of the device and the top p-GaN layers were grown by metal organic chemical vapor deposition and highly doped n-GaN was grown by NH3 molecular beam epitaxy to form the TJ. The regrowth interface in these hybrid devices was found to have a high concentration of oxygen, which likely enhanced tunneling through the diode. For optimized regrowth, the best tunnel junction device had a total differential resistivity of 1.5 × 10−4 Ω cm2, including contact resistance. As a demonstration, a blue-light-emitting diode on a ($20\\\\bar{2}\\\\bar{1}$) GaN substrate with a hybrid tunnel junction and an n-GaN current spreading layer was fabricated and compared with a reference sample with a transparent conducting oxide (TCO) layer. The tunnel junction LED showed a lower forward operating voltage and a higher efficiency at a low current density than the TCO LED.

  20. Hybrid tunnel junction contacts to III–nitride light-emitting diodes

    KAUST Repository

    Young, Erin C.; Yonkee, Benjamin P.; Wu, Feng; Oh, Sang Ho; DenBaars, Steven P.; Nakamura, Shuji; Speck, James S.

    2016-01-01

    In this work, we demonstrate highly doped GaN p–n tunnel junction (TJ) contacts on III–nitride heterostructures where the active region of the device and the top p-GaN layers were grown by metal organic chemical vapor deposition and highly doped n-GaN was grown by NH3 molecular beam epitaxy to form the TJ. The regrowth interface in these hybrid devices was found to have a high concentration of oxygen, which likely enhanced tunneling through the diode. For optimized regrowth, the best tunnel junction device had a total differential resistivity of 1.5 × 10−4 Ω cm2, including contact resistance. As a demonstration, a blue-light-emitting diode on a ($20\\bar{2}\\bar{1}$) GaN substrate with a hybrid tunnel junction and an n-GaN current spreading layer was fabricated and compared with a reference sample with a transparent conducting oxide (TCO) layer. The tunnel junction LED showed a lower forward operating voltage and a higher efficiency at a low current density than the TCO LED.

  1. Experimental study of poloidal flow effect on magnetic island dynamics in LHD and TJ-II

    International Nuclear Information System (INIS)

    Narushima, Y.; Sakakibara, S.; Castejon, F.

    2010-11-01

    The dynamics of a magnetic island are studied by focusing on the poloidal flows in the helical devices LHD and TJ-II. The temporal increment of the ExB poloidal flow prior to the magnetic island transition from growth to healing is observed. The direction of the poloidal flow is in the electron-diamagnetic direction in LHD and in the ion-diamagnetic direction in TJ-II. From the magnetic diagnostics, it is observed that a current structure flowing in the plasma moves ∼π rad poloidally in the electron-diamagnetic direction during the transition in LHD experiments. These experimental observations from LHD and TJ-II show that the temporal increment of the poloidal flow is followed by the transition (growth to healing) of the magnetic island regardless of the flow direction and clarify the fact that significant poloidal flow affects the magnetic island dynamics. (author)

  2. The TJ-II Relational Database Access Library: A User's Guide

    International Nuclear Information System (INIS)

    Sanchez, E.; Portas, A. B.; Vega, J.

    2003-01-01

    A relational database has been developed to store data representing physical values from TJ-II discharges. This new database complements the existing TJ-EI raw data database. This database resides in a host computer running Windows 2000 Server operating system and it is managed by SQL Server. A function library has been developed that permits remote access to these data from user programs running in computers connected to TJ-II local area networks via remote procedure cali. In this document a general description of the database and its organization are provided. Also given are a detailed description of the functions included in the library and examples of how to use these functions in computer programs written in the FORTRAN and C languages. (Author) 8 refs

  3. Iterative noise removal from temperature and density profiles in the TJ-II Thomson scattering

    International Nuclear Information System (INIS)

    Farias, G.; Dormido-Canto, S.; Vega, J.; Santos, M.; Pastor, I.; Fingerhuth, S.; Ascencio, J.

    2014-01-01

    TJ-II Thomson Scattering diagnostic provides temperature and density profiles of plasma. The CCD camera acquires images that are corrupted with some kind of noise called stray-light. This noise degrades both image contrast and measurement accuracy, which could produce unreliable profiles of the diagnostic. So far, several approaches have been applied in order to decrease the noise in the TJ-II Thomson scattering images. Since the presence of the noise is not global but located in some particular regions of the image, advanced processing techniques are needed. However such methods require of manual fine-tuning of parameters to reach a good performance. In this contribution, an iterative image processing approach is applied in order to reduce the stray light effects in the images of the TJ-II Thomson scattering diagnostic. The proposed solution describes how the noise can be iteratively reduced in the images when a key parameter is automatically adjusted during the iterative process

  4. Iterative noise removal from temperature and density profiles in the TJ-II Thomson scattering

    Energy Technology Data Exchange (ETDEWEB)

    Farias, G., E-mail: gonzalo.farias@ucv.cl [Pontificia Universidad Católica de Valparaíso, Av. Brasil 2147, Valparaíso (Chile); Dormido-Canto, S., E-mail: sebas@dia.uned.es [Departamento de Informática y Automática, UNED, 28040 Madrid (Spain); Vega, J., E-mail: jesus.vega@ciemat.es [Asociación EURATOM/CIEMAT para Fusión, Avd. Complutense 22, 28040 Madrid (Spain); Santos, M., E-mail: msantos@ucm.es [Departamento de Arquitectura de Computadores y Automática, Universidad Complutense de Madrid, 28040 Madrid (Spain); Pastor, I., E-mail: ignacio.pastor@ciemat.es [Asociación EURATOM/CIEMAT para Fusión, Avd. Complutense 22, 28040 Madrid (Spain); Fingerhuth, S., E-mail: sebastian.fingerhuth@ucv.cl [Pontificia Universidad Católica de Valparaíso, Av. Brasil 2147, Valparaíso (Chile); Ascencio, J., E-mail: j_ascencio21@hotmail.com [Pontificia Universidad Católica de Valparaíso, Av. Brasil 2147, Valparaíso (Chile)

    2014-05-15

    TJ-II Thomson Scattering diagnostic provides temperature and density profiles of plasma. The CCD camera acquires images that are corrupted with some kind of noise called stray-light. This noise degrades both image contrast and measurement accuracy, which could produce unreliable profiles of the diagnostic. So far, several approaches have been applied in order to decrease the noise in the TJ-II Thomson scattering images. Since the presence of the noise is not global but located in some particular regions of the image, advanced processing techniques are needed. However such methods require of manual fine-tuning of parameters to reach a good performance. In this contribution, an iterative image processing approach is applied in order to reduce the stray light effects in the images of the TJ-II Thomson scattering diagnostic. The proposed solution describes how the noise can be iteratively reduced in the images when a key parameter is automatically adjusted during the iterative process.

  5. Kauppakaveri : Tjänsteutveckling åt de äldre

    OpenAIRE

    Haarala, Eveliina

    2013-01-01

    Det verkar som att tjänsteutveckling på senare tid blivit något som man anser trendigt. Jag deltog i ett par kurser i ämnet som var samordnade med Arcada och Svenska social- och kommunalhögskolan. I den ena av kurserna fick jag delta i en tjänsteutvecklingsprocess. Det här examensarbetets syfte är att rapportera om utvecklingsprocessen för en tjänst som är formgiven åt åldringar. Problemområdet i arbetet ligger inom de ensamma åldringarna och affärslivet som borde vakna upp och betjäna dem me...

  6. Two-dimensional full-wave code for reflectometry simulations in TJ-II

    International Nuclear Information System (INIS)

    Blanco, E.; Heuraux, S.; Estrada, T.; Sanchez, J.; Cupido, L.

    2004-01-01

    A two-dimensional full-wave code in the extraordinary mode has been developed to simulate reflectometry in TJ-II. The code allows us to study the measurement capabilities of the future correlation reflectometer that is being installed in TJ-II. The code uses the finite-difference-time-domain technique to solve Maxwell's equations in the presence of density fluctuations. Boundary conditions are implemented by a perfectly matched layer to simulate free propagation. To assure the stability of the code, the current equations are solved by a fourth-order Runge-Kutta method. Density fluctuation parameters such as fluctuation level, wave numbers, and correlation lengths are extrapolated from those measured at the plasma edge using Langmuir probes. In addition, realistic plasma shape, density profile, magnetic configuration, and experimental setup of TJ-II are included to determine the plasma regimes in which accurate information may be obtained

  7. Molecular electronic junction transport

    DEFF Research Database (Denmark)

    Solomon, Gemma C.; Herrmann, Carmen; Ratner, Mark

    2012-01-01

    Whenasinglemolecule,oracollectionofmolecules,isplacedbetween two electrodes and voltage is applied, one has a molecular transport junction. We discuss such junctions, their properties, their description, and some of their applications. The discussion is qualitative rather than quantitative, and f...

  8. Electron temperature and density profiles measurement in the TJ-1 tokamak by Thomson scattering

    International Nuclear Information System (INIS)

    Pardo, C.; Zurro, B.

    1986-01-01

    Electron temperature and density profiles of ohmically heated hydrogen plasmas in the TJ-1 tokamak have been measured by Thomson scattering. The temperature profile peaks sharply in the central region while the density profile is very flat. Temperature values between 100 and 390 eV have been measured for densities in the range of 5.10 12 to 2.6.10 13 cm -3 . Parameters characterizing TJ-1 plasma, such as confinement times Z eff , have been deduced from experimental data. Energy confinement times are compared with experimental scaling laws. (author)

  9. Plasma density determination by microwave interferometry. The 2 mm interferometer of the TJ-1 tokamak

    International Nuclear Information System (INIS)

    Manero, F.; Martin, R.

    1984-01-01

    In this paper a description is given of the microwave interferometer used for measuring the plasma electronic density in the TJ-1 Tokamak of Fusion Division of JEN. The principles of the electronic density measurement are discussed in detail, as well as those concerning the determination of density profiles from experimental data. A description of the interferometer used in the TJ-1 Tokamak is given, together with a detailed analysis of the circuits which constitute the measuring chain. The working principles of the klystron reflex and hybrid rings are also presented. (author)

  10. Controlling Confinement with Induced Toroidal Current in the Flexible Heliac TJ-II

    Energy Technology Data Exchange (ETDEWEB)

    Romero, J A; Lopez-Bruna, D; Lopez-Fraguas, A; Ascasibar, E; TJ-II Team

    2002-07-01

    A method to control plasma particle an energy confinement in the TJ-II Heliac devices is reported A small toroidal current is induced in the plasma with the aid of a 0.2 Wb air core transformer. Plasma particle and energy confinement improve (degrade) with negative (positive) plasma current. For typical TJ-II discharges plasma density and temperature broaden considerably when plasma current is sufficiently negative, accounting for a 40% increase in stored energy. The experimental results agree qualitatively with the paradigm of instability growth rate modifications with magnetic shear. (Author) 18 refs.

  11. Plasma-wall Interaction Studies in the Start-up Phase of TJ-II

    International Nuclear Information System (INIS)

    De la Cal, E.; Tabares, F.L.; Tafalla, D.

    1998-01-01

    The aim of this work is to present some first plasma-wall interaction studies made during the first experimental campaign of TJ-II. The different sections contain independent contributions presented orally in the fusion division of the Euratom-Ciemat association during 1998: I. Density limit during the start-up phase of TJ-II : are we limited by radiation?. II. Temporal evolution of oxygen in the plasma during an experimental day. III. The contribution of helium to the plasma electron density IV. First studies of the S.O.L. diffusion coefficient and its dependence with the boundary plasma parameters. (Author) 3 refs

  12. Controlling Confinement with Induced Toroidal Current in the Flexible Heliac TJ-II

    International Nuclear Information System (INIS)

    Romero, J. A.; Lopez-Bruna, D.; Lopez-Fraguas, A.; Ascasibar, E.; TJ-II Team

    2002-01-01

    A method to control plasma particle an energy confinement in the TJ-II Heliac devices is reported A small toroidal current is induced in the plasma with the aid of a 0.2 Wb air core transformer. Plasma particle and energy confinement improve (degrade) with negative (positive) plasma current. For typical TJ-II discharges plasma density and temperature broaden considerably when plasma current is sufficiently negative, accounting for a 40% increase in stored energy. The experimental results agree qualitatively with the paradigm of instability growth rate modifications with magnetic shear. (Author) 18 refs

  13. Plasma density determination by microwave interferometry .- The 2 mm interferometer of the TJ-1 Tokamak

    International Nuclear Information System (INIS)

    Martin, R.; Manero, F.

    1984-01-01

    In this paper a description is given of the microwave interferometer used for measuring the plasma electronic density in the TJ-1 Tokamak of Fusion Division of JEN. The principles of the electronic density measurement are discussed in detail, as well as those concerning the determination of density pro files from experimental data. A description of the interferometer used in the TJ-1 Tokamak is given, together with a detailed analysis of the circuits which constitute the measuring chain. The working principles of the klystron reflex and hybrid rings are also presented. (Author) 23 refs

  14. Exit points, on plasma, of lost fast ions during NBI in TJ-II

    International Nuclear Information System (INIS)

    Guasp, J.

    1995-09-01

    The distribution of the exit points, on plasma border, for the lost fast ions during tangential balanced NBI in TJ-II helical axis Stellarator is theoretically analysed, as well for direct as for delayed losses. The link between the position of those exit points and the corresponding at birth, orbits and drifts is analysed also, it is shown that such relation is rather independent of beam energy and plasma density and is mainly related to the magnetic configuration characteristics. This study is a needed intermediate step to the analysis of impacts of those ions on the vacuum vessel of TJ-II

  15. Exit points, on plasma, of lost fast ions during NBI in TJ-II

    International Nuclear Information System (INIS)

    Guasp, J.

    1995-01-01

    The distribution of the exit points, on plasma border, for the lost fast ions during tangential balanced NBI in TJ-II helical axis Stellarator is theoretically analysed, as well for direct as for delayed losses. The link between, the position of those exit points and the corresponding at birth, orbits and drifts is analysed also. It is shown that such relation is rather independent of beam energy and plasma density and is mainly related to the magnetic configuration characteristics. This study is a needed intermediate step to the analysis of impacts of those ions on the vacuum vessel of TJ-II. (Author) 2 refs

  16. Calculation of the magnetic vector potential in the TJ-II; Calculo del Potencial Magnetico Vector en el TJ-II

    Energy Technology Data Exchange (ETDEWEB)

    Lopez Fraguas, A.; Lopez Bruna, D.; Romero, J. A.

    2005-07-01

    The properties of the vector magnetic potential and its usefulness to calculate magnetic fluxes in both stationary and time-dependent conditions are p revised in this report. We have adapted to the TJ-II Flexible Heliac efficient numerical expressions to calculate the vector potential, calculating in addition the magnetic flux with this formalism in circumstances whose complexity makes very convenient the use of the vector potential. The result on induced voltages offer theoretical support to the measurements of induced voltage due to the OH coils in the plasma, like the measurements provided by the loop voltage diagnostic installed in the TJ-II, as well as to the cylindrical approximation of the plasma often used to interpret experimental data. (Author) 11 refs.

  17. [Effect of eicosapentaenoic acid on mRNA expression of tight junction protein ZO-1 in intestinal epithelial cells after Escherichia coli LF82 infection].

    Science.gov (United States)

    Hao, Li-Jun; Lin, Yan; Zhang, Wei; Tian, Jiao; Wang, Ya; Chen, Peng-De; Hu, Chong-Kang; Zeng, Ling-Chao; Yang, Jie; Wang, Bao-Xi; Jiang, Xun

    2017-06-01

    To investigate the change in the expression of tight junction protein ZO-1 in intestinal epithelial cells (Caco-2 cells) and the protective effect of eicosapentaenoic acid (EPA) after adherent-invasive Escherichia coli (E.coli) LF82 infection. The Caco-2 cell line was used to establish an in vitro model of tight junction of intestinal epithelial cells. Caco-2 cells were divided into EPA treatment groups (0, 25, 50, 100, and 200 μmol/L EPA) and EPA (0, 25, 50, 100, and 200 μmol/L EPA)+E.coli LF82 treatment (0, 6, and 12 hours) groups. A microscope was used to observe the morphological characteristics of the cells. MTT assay was used to determine the cell growth curve. The activity of alkaline phosphatase (ALP) at both sides of the cell membrane was compared to evaluate the Caco-2 cell model. MTT assay and flow cytometry were used to investigate the effects of different concentrations of EPA on the survival rate and apoptosis rate of Caco-2 cells. RT-qPCR was used to measure the mRNA expression of ZO-1 in Caco-2 cells after EPA and/or E.coli LF82 treatment. ELISA was used to measure the change in the level of tumor necrosis factor-α (TNF-α) in culture supernatant. After EPA treatment (25 and 50 μmol/L), the proliferation of Caco-2 cells was induced in a dose-dependent manner. The survival rates of the cells were significantly higher than those in the control group (PE.coli LF82 treatment groups had decreasing mRNA expression of ZO-1 in Caco-2 cells over the time of treatment and had significantly lower mRNA expression of ZO-1 than the untreated group (PE.coli LF82 and 25 or 50 μmol/L EPA for 6 or 12 hours showed an increase in the mRNA expression of ZO-1 with the increasing concentration of EPA, as well as significantly higher mRNA expression of ZO-1 than the Caco-2 cells treated with E.coli LF82 alone (PE.coli LF82 alone for 6 or 12 hours had increasing secretion of TNF-α over the time of treatment and had significantly higher secretion than the untreated

  18. Geometry of the TJ-II in Astra 6.0; Geometria del TJ-II en Astra 6.0

    Energy Technology Data Exchange (ETDEWEB)

    Lopez-Bruna, D.; Romero, J.A.; Castejon, F.

    2006-07-01

    One of the most exploited features of the TJ-II Heliac, a facility in the Laboratorio Nacional de Fusion (CIEMAT, Madrid), is its ability to explore plasmas in different magnetic configurations. For this reason, there are available libraries that provide the metrics and associated magnitudes for many among all possible configurations. On the other hand, the transport codes that can normally be used to perform transport calculations cannot dea properly with these geometries, which is especially delicate when there are induced plasma currents. In the present work we adopt ASTRA, a transport analysis shell, to study the approximations performed when calculations that impose axi-symmetry (as ASTRA does) are performed on magnetic configurations that are not really axi-symmetric. After describing how we obtain those TJ-II metric averages that must be set in ASTRA, we perform two comparisons: (i) we obtain the vacuum rotational transform as deduced from the metric coefficients but imposing axisymmetry, and compare the results with the rotational transform yielded by the existing libraries; and (ii) we build a ID transport code with TJ-II metrics so its results can be compared with those of ASTRA. In both cases, the differences found indicate that evaluating the evolution of the rotational transform under ohmic induction and transport evolution is acceptable assuming that the geometry itself does not evolve. (Author) 11 refs.

  19. Search for Quasi-isodynamic Effects in TJ-II; Busqueda de efectos quasi-isodinamicos en el TJ-II

    Energy Technology Data Exchange (ETDEWEB)

    Guasp, J.; Liniers, M. [Ciemat. Madrid (Spain)

    2000-07-01

    The possibility of quasi-isodynamics effects (QID) in the TJ-II helical axis Stellarator has been explored maintaining the present setting for the toroidal field coils (TFC). In order to do this it has been necessary to implement a new method of calculation, using real space coordinates to follow the particle trajectories, instated the Boozer coordinates as was usual formerly. The result for the exploration of the flexibility diagram of TJ-II, including magnetic axis a shift effects, has been negative. It seems that there are not useful QID regions in TJ-II with the present setting of TFC carrying equal currents in all coils. Nevertheless, in spite of this negative result, the calculation in real space and, mainly, the grater number of configurations analysed, have produced a series of new important results, some of them unexpected. The influence of rational surfaces is very important. Optima and minima of confinement alternate at both sides of the rational values (mainly for the 1/2 by period) in a way very similar to the radial electric field resonance cases. This effect originates in the peculiar orbit topology in the presence of diffusion. Some lines of study are proposed to deal with this problem. Finally, the negative result of the QID search suggests the convenience to start a similar search without the restriction of equal currents on all the TEC. (Author) 18 refs.

  20. Mycobacterium tuberculosis nucleoid-associated DNA-binding protein H-NS binds with high-affinity to the Holliday junction and inhibits strand exchange promoted by RecA protein.

    Science.gov (United States)

    Sharadamma, N; Harshavardhana, Y; Singh, Pawan; Muniyappa, K

    2010-06-01

    A number of studies have shown that the structure and composition of bacterial nucleoid influences many a processes related to DNA metabolism. The nucleoid-associated proteins modulate not only the DNA conformation but also regulate the DNA metabolic processes such as replication, recombination, repair and transcription. Understanding of how these processes occur in the context of Mycobacterium tuberculosis nucleoid is of considerable medical importance because the nucleoid structure may be constantly remodeled in response to environmental signals and/or growth conditions. Many studies have concluded that Escherichia coli H-NS binds to DNA in a sequence-independent manner, with a preference for A-/T-rich tracts in curved DNA; however, recent studies have identified the existence of medium- and low-affinity binding sites in the vicinity of the curved DNA. Here, we show that the M. tuberculosis H-NS protein binds in a more structure-specific manner to DNA replication and repair intermediates, but displays lower affinity for double-stranded DNA with relatively higher GC content. Notably, M. tuberculosis H-NS was able to bind Holliday junction (HJ), the central recombination intermediate, with substantially higher affinity and inhibited the three-strand exchange promoted by its cognate RecA. Likewise, E. coli H-NS was able to bind the HJ and suppress DNA strand exchange promoted by E. coli RecA, although much less efficiently compared to M. tuberculosis H-NS. Our results provide new insights into a previously unrecognized function of H-NS protein, with implications for blocking the genome integration of horizontally transferred genes by homologous and/or homeologous recombination.

  1. Junction and circuit fabrication

    International Nuclear Information System (INIS)

    Jackel, L.D.

    1980-01-01

    Great strides have been made in Josephson junction fabrication in the four years since the first IC SQUID meeting. Advances in lithography have allowed the production of devices with planar dimensions as small as a few hundred angstroms. Improved technology has provided ultra-high sensitivity SQUIDS, high-efficiency low-noise mixers, and complex integrated circuits. This review highlights some of the new fabrication procedures. The review consists of three parts. Part 1 is a short summary of the requirements on junctions for various applications. Part 2 reviews intergrated circuit fabrication, including tunnel junction logic circuits made at IBM and Bell Labs, and microbridge radiation sources made at SUNY at Stony Brook. Part 3 describes new junction fabrication techniques, the major emphasis of this review. This part includes a discussion of small oxide-barrier tunnel junctions, semiconductor barrier junctions, and microbridge junctions. Part 3 concludes by considering very fine lithography and limitations to miniaturization. (orig.)

  2. Ischemic preconditioning enhances integrity of coronary endothelial tight junctions

    Energy Technology Data Exchange (ETDEWEB)

    Li, Zhao [Department of Pharmaceutical Sciences, College of Pharmacy, South Dakota State University, Brookings, SD 57007 (United States); Jin, Zhu-Qiu, E-mail: zhu-qiu.jin@sdstate.edu [Department of Pharmaceutical Sciences, College of Pharmacy, South Dakota State University, Brookings, SD 57007 (United States)

    2012-08-31

    Highlights: Black-Right-Pointing-Pointer Cardiac tight junctions are present between coronary endothelial cells. Black-Right-Pointing-Pointer Ischemic preconditioning preserves the structural and functional integrity of tight junctions. Black-Right-Pointing-Pointer Myocardial edema is prevented in hearts subjected to ischemic preconditioning. Black-Right-Pointing-Pointer Ischemic preconditioning enhances translocation of ZO-2 from cytosol to cytoskeleton. -- Abstract: Ischemic preconditioning (IPC) is one of the most effective procedures known to protect hearts against ischemia/reperfusion (IR) injury. Tight junction (TJ) barriers occur between coronary endothelial cells. TJs provide barrier function to maintain the homeostasis of the inner environment of tissues. However, the effect of IPC on the structure and function of cardiac TJs remains unknown. We tested the hypothesis that myocardial IR injury ruptures the structure of TJs and impairs endothelial permeability whereas IPC preserves the structural and functional integrity of TJs in the blood-heart barrier. Langendorff hearts from C57BL/6J mice were prepared and perfused with Krebs-Henseleit buffer. Cardiac function, creatine kinase release, and myocardial edema were measured. Cardiac TJ function was evaluated by measuring Evans blue-conjugated albumin (EBA) content in the extravascular compartment of hearts. Expression and translocation of zonula occludens (ZO)-2 in IR and IPC hearts were detected with Western blot. A subset of hearts was processed for the observation of ultra-structure of cardiac TJs with transmission electron microscopy. There were clear TJs between coronary endothelial cells of mouse hearts. IR caused the collapse of TJs whereas IPC sustained the structure of TJs. IR increased extravascular EBA content in the heart and myocardial edema but decreased the expression of ZO-2 in the cytoskeleton. IPC maintained the structure of TJs. Cardiac EBA content and edema were reduced in IPC hearts. IPC

  3. A real-time current driving control system for the TJ-II coils

    International Nuclear Information System (INIS)

    Pena, Angel de la; Pacios, Luis; Carrasco, Ricardo; Lapayese, Fernando

    2009-01-01

    Since the start of plasma operation in the TJ-II stellarator, the required values of DC currents that are fed to its different coil sets have been controlled to high precision along the complete discharge flat-top. As a result each current configuration produced a highly stable magnetic-field configuration. Recently, the configurational flexibility of the TJ-II has been broadened by the commissioning of a new mode of operation that allows magnetic configurations to be varied dynamically during the discharge flat-top. In order to achieve this, new hardware and software features have been added to the TJ-II Control System. These new features may also provide new strategies for feedback control in accordance with parameters measured in one or more diagnostics. In this new set-up, coil current profiles are generated and controlled to millisecond timescales by a system based on VMEbus and OS9 real-time operating system. A new communication middleware architecture called XML-based Messages Distribution Service (X-MDS) has been designed to exchange XML-based data with calling clients. Furthermore, with this software, a fully functional Java application for supervision and for current profiles settings has been developed. This paper provides a detailed description of the complete TJ-II real-time current-profile control system and results obtained during its operation.

  4. The spectrometer of the High-Resolution Multi position Thomson Scattering Diagnostic for TJ-II

    International Nuclear Information System (INIS)

    Herranz, J.; Barth, C. J.; Castejon, F.; Lopez-Sanchez, A.; Mirones, E.; Pastor, I.; Perez, D.; Rodriguez, C.

    2001-01-01

    Since 1998, a high-resolution multiposition thompson scattering system is in operation at the stellarator TJ-II, combining high accuracy and excellent spatial resolution. A description of the diagnostic spectrometer is presented. The main characteristics of the spectrometer that allow YJ-II Thomson scattering diagnostic to have high spatial and spectral resolution are described in this paper. (Author)

  5. Applying a message oriented middleware architecture to the TJ-II remote participation system

    International Nuclear Information System (INIS)

    Sanchez, E.; Portas, A.; Pereira, A.; Vega, J.

    2006-01-01

    A message oriented middleware (MOM) has been introduced into the TJ-II data acquisition system to on-line distribute information. Java message service (JMS) has been chosen as the messaging application program interface (API) in order to ensure multiplatform portability. A library of C++ classes providing interface for JMS Java classes has been developed. This allows C++ programs to inter-communicate through JMS. In addition, a set of C wrapper functions has also been developed to provide basic messaging functionalities for C or FORTRAN programs. These functions are used in TJ-II LabView data acquisition applications. Several software applications that take advantage of the MOM architecture have been developed. Firstly, a general-user application allows monitoring of the data acquisition systems. Secondly, a simple application permits the visualization of TJ-II monitor signals with on-line data refreshing. These applications are written in the Java language, thereby ensuring its portability. These software tools provide new functionalities to the TJ-II remote participation system and are equally used in the local environment

  6. Microwave reflectrometry for electron density measurements in the TJ-1 tokamak plasma

    International Nuclear Information System (INIS)

    Anabitarte, E.; Bustamante, E.G.; Calderon, M.A.G.; Vegas, A.

    1986-01-01

    A study about microwave reflectometry to measure the outside profile of the electron plasma density on tokamak TJ-1 is presented. It is also presented the condition of applicability of this method after the characteristic parameters of the plasma and its resolution. The simulation of the plasma in laboratory by means of a metallic mirror causes the whole characterization of the reflectometer. (author)

  7. Dust visualisation in TJ-II with intensified visible fast cameras

    International Nuclear Information System (INIS)

    Cal, E. de la; Pablos, J. L. de; Carralero, D.; Hidalgo, C.

    2010-01-01

    A visible fast camera equipped with an image Intensifier and atomic line filters is used in TJ-II for spectroscopic dust observation. First results show characteristic features depending on filter and clearly differing from those without the filters as is usually done in existing experiments. Preliminary discussions of the observed results are presented. (Author) 5 refs.

  8. Low-temperature specific heat of the degenerate supersymmetric t-J model in one dimension

    International Nuclear Information System (INIS)

    Lee, K.; Schlottmann, P.

    1996-01-01

    We consider the one-dimensional SU(N)-invariant t-J model, which consists of electrons with N spin components on a lattice with nearest-neighbor hopping t constrained by the excluded multiple occupancy of the sites and spin-exchange J between neighboring lattice sites. The model is integrable and has been diagonalized in terms of nested Bethe ansatze at the supersymmetric point t=J. The low-T specific heat is proportional to T. The γ-coefficient is extracted from the thermodynamic Bethe-ansatz equations and is expressed in terms of the spin wave velocities and the group velocity of the charges for arbitrary N, band filling, and splitting of the levels (magnetic and crystalline fields). Our results contain the following special cases: (i) For N=2 the traditional spin-1/2 supersymmetric t-J model, (ii) for exactly one electron per site the SU(N)-Heisenberg chain, and (iii) for N=4 the two-band supersymmetric t-J model with crystalline field splitting. copyright 1996 American Institute of Physics

  9. A real-time current driving control system for the TJ-II coils

    Energy Technology Data Exchange (ETDEWEB)

    Pena, Angel de la [Association EURATOM - CIEMAT, Avda. Complutense 22, 28040 Madrid (Spain)], E-mail: a.delapena@ciemat.es; Pacios, Luis; Carrasco, Ricardo; Lapayese, Fernando [Association EURATOM - CIEMAT, Avda. Complutense 22, 28040 Madrid (Spain)

    2009-06-15

    Since the start of plasma operation in the TJ-II stellarator, the required values of DC currents that are fed to its different coil sets have been controlled to high precision along the complete discharge flat-top. As a result each current configuration produced a highly stable magnetic-field configuration. Recently, the configurational flexibility of the TJ-II has been broadened by the commissioning of a new mode of operation that allows magnetic configurations to be varied dynamically during the discharge flat-top. In order to achieve this, new hardware and software features have been added to the TJ-II Control System. These new features may also provide new strategies for feedback control in accordance with parameters measured in one or more diagnostics. In this new set-up, coil current profiles are generated and controlled to millisecond timescales by a system based on VMEbus and OS9 real-time operating system. A new communication middleware architecture called XML-based Messages Distribution Service (X-MDS) has been designed to exchange XML-based data with calling clients. Furthermore, with this software, a fully functional Java application for supervision and for current profiles settings has been developed. This paper provides a detailed description of the complete TJ-II real-time current-profile control system and results obtained during its operation.

  10. Hazardous Waste Cleanup: IBM Corporation-TJ Watson Research Center in Yorktown Heights, New York

    Science.gov (United States)

    IBM Corporation -TJ Watson Research Center is located in southern Yorktown near the boundary separating the Town of Yorktown from the Town of New Castle. The site occupies an area of approximately 217 acres and adjoins land uses are predominantly residenti

  11. A method for measuring plasma position in TJ-I Tokamak

    International Nuclear Information System (INIS)

    Quin, J.; TJ-I Team.

    1993-01-01

    A method using pairs of Mirnov coils to measure the plasma position in TJ-I is presented. The simple toroidal filament model which neglects the effect of plasma current density profile has proven to be acceptable within the experimental accuracy. The effect of plasma current density profile remains to be small, if the plasma current density profile has a quadratic form. (Author)

  12. On the role of impurity radiation on edge turbulence in the TJ-1 Tokamak

    International Nuclear Information System (INIS)

    Ochando, M.A.; Pedrosa, M.A.; Balbin, R.; Garcia-Cortes, I.; Hidalgo, C.

    1994-01-01

    The correlation between edge radiation and electron temperature and density fluctuations has been studied in the vicinity of the upper poloidal limiter of the TJ-I tokamak. When edge impurity radiation is strongly raked in the proximity of the limiter radius, electron temperature fluctuations are notably higher than density fluctuations. Results provide experimental evidence of edge turbulence driven by thermal instabilities

  13. Monte Carlo estimation of neoclassical transport for the TJ-II stellarator

    International Nuclear Information System (INIS)

    Tribaldos, V.

    2001-01-01

    The neoclassical transport properties of TJ-II stellarator [C. Alejaldre et al., Fusion Technol. 13, 521 (1988)] are studied with the monoenergetic Monte Carlo technique. A compromise between the number of modes and particles and the required computing time to obtain reliable estimates, from the computational point of view, of the monoenergetic diffusion coefficients is shown to be of one thousand particles and one hundred harmonics, because of the rich magnetic-field structure of TJ-II. Although, these requirements are probably too demanding in making the transport estimations. The data base containing the normalized monoenergetic diffusion coefficient for several radial positions, radial electric fields and collisionalities have been fitted using a neural network. This fit reduces the number of points necessary in the data base and allows a smooth interpolation and extrapolation to perform the convolutions of the monoenergetic coefficients with the Maxwellian. For two different typical TJ-II discharges the ambipolar radial electric field, and the neoclassical particle and heat fluxes are presented both showing rather large positive radial electric fields at the plasma core and small negative fields at the edge. The neoclassical particle and energy confinement time are in surprisingly good agreement with the experimental energy balance analysis and the international stellarator scaling. Although no satisfactory explanation is available yet the large neoclassical diffusion caused by the complex ripple structure of TJ-II magnetic field may be an important ingredient

  14. Microcomputer based system to control the load of a capacitor array in the TJ-1 Tokamak

    International Nuclear Information System (INIS)

    Alberdi Primicia, J.; Asenjo, L.; Sanz, J.A.

    1990-01-01

    The power to create the magnetic field in the TJ-1 TOKAMAK is provide by an array of 16 capacitor sets. The total capacity of this array is 8.1F. This work describes a computer system based on the Motorola M-6800 microprocessor which controls the load of the capacitor set-and establishes the conditions for the reactor trigger. (author)

  15. Microcomputer Based System to control the Load of a Capacitor Array in the TJ-1 Tokamak

    International Nuclear Information System (INIS)

    Alberdi, J.; Asenso, L.; Sanz, J. A.

    1990-01-01

    The power to create the magnetic fields in the TJ-1 Tokamak is provides by an array of 16 capacitor sets. The total capacity of this array is 8. 1F. This work describes a computer system based on the Motorola M-6800 micro- processor which controls the load of the capacitor set and stablished the conditions for the reactor trigger. (Author)

  16. Dust visualisation in TJ-II with intensified visible Fast Cameras

    Energy Technology Data Exchange (ETDEWEB)

    Cal, E. de la; Pablos, J. L. de; Carralero, D.; Hidalgo, C.

    2010-10-21

    A visible fast camera equipped with an image Intensifier and atomic line filters is used in TJ-II for spectroscopic dust observation. First results show characteristic features depending on filter and clearly differing from those without the filters as is usually done in existing experiments. Preliminary discussions of the observed results are presented. (Author) 5 refs.

  17. The T-J model and superconductivity | Umo | Global Journal of Pure ...

    African Journals Online (AJOL)

    The t-J model written in terms of Hubbard operators is studied with a view to contributing to the search for the mechanism of high temperature superconductivity in the cuprates. The method of irreducible Green function is used to obtain the spectrum of quasiparticles excitation and d-wave pairing gap function.

  18. Advanced Design of the First Quasi-optical Transmission Line for ECRH at TJ-II

    International Nuclear Information System (INIS)

    Fernandez, A.; Likin, K.; Martin, R.

    1999-01-01

    TJ-II plasma start-up and heating are made by electron cyclotron resonance waves at the second harmonic of the electron cyclotron frequency. The microwave power of the gyrotrons is transmitted by two quasi-optical transmission lines. The first line launches the microwave power under fixed injection geometry, i. e. there is no a possibility to change the launching angle the wave polarization. Due to the long distance between the last focusing mirror and the center TJ-II vessel the beam is quite wide at plasma border. The second line has a moveable mirror installed inside the TJ-II vessel. To get high absorption efficiency and a narrow energy deposition profile the internal mirror focuses the wave beam at plasma center. The beam width is about 2 cm. To get more flexibility in experiments on heating and current drive the first transmission line needs to be upgraded. The designs is presented in this report. It includes and internal mirror to focus the beam and to change the injection angle. A polarizer consisting in two corrugated mirrors will be incorporated to get any wave polarization. Two mirrors with an array of coupling holes and calorimetric measurements of the energy absorbed in the barrier window will permit the estimation of the microwave power launched the TJ-II. (Author) 13 refs

  19. Applying a message oriented middleware architecture to the TJ-II remote participation system

    Energy Technology Data Exchange (ETDEWEB)

    Sanchez, E. [Asociacion EURATOM/CIEMAT para Fusion, Avda Complutense 22, 28040 Madrid (Spain)]. E-mail: edi.sanchez@ciemat.es; Portas, A. [Asociacion EURATOM/CIEMAT para Fusion, Avda Complutense 22, 28040 Madrid (Spain); Pereira, A. [Asociacion EURATOM/CIEMAT para Fusion, Avda Complutense 22, 28040 Madrid (Spain); Vega, J. [Asociacion EURATOM/CIEMAT para Fusion, Avda Complutense 22, 28040 Madrid (Spain)

    2006-07-15

    A message oriented middleware (MOM) has been introduced into the TJ-II data acquisition system to on-line distribute information. Java message service (JMS) has been chosen as the messaging application program interface (API) in order to ensure multiplatform portability. A library of C++ classes providing interface for JMS Java classes has been developed. This allows C++ programs to inter-communicate through JMS. In addition, a set of C wrapper functions has also been developed to provide basic messaging functionalities for C or FORTRAN programs. These functions are used in TJ-II LabView data acquisition applications. Several software applications that take advantage of the MOM architecture have been developed. Firstly, a general-user application allows monitoring of the data acquisition systems. Secondly, a simple application permits the visualization of TJ-II monitor signals with on-line data refreshing. These applications are written in the Java language, thereby ensuring its portability. These software tools provide new functionalities to the TJ-II remote participation system and are equally used in the local environment.

  20. An event-oriented database for continuous data flows in the TJ-II environment

    Energy Technology Data Exchange (ETDEWEB)

    Sanchez, E. [Asociacion Euratom/CIEMAT para Fusion Madrid, 28040 Madrid (Spain)], E-mail: edi.sanchez@ciemat.es; Pena, A. de la; Portas, A.; Pereira, A.; Vega, J. [Asociacion Euratom/CIEMAT para Fusion Madrid, 28040 Madrid (Spain); Neto, A.; Fernandes, H. [Associacao Euratom/IST, Centro de Fusao Nuclear, Avenue Rovisco Pais P-1049-001 Lisboa (Portugal)

    2008-04-15

    A new database for storing data related to the TJ-II experiment has been designed and implemented. It allows the storage of raw data not acquired during plasma shots, i.e. data collected continuously or between plasma discharges while testing subsystems (e.g. during neutral beam test pulses). This new database complements already existing ones by permitting the storage of raw data that are not classified by shot number. Rather these data are indexed according to a more general entity entitled event. An event is defined as any occurrence relevant to the TJ-II environment. Such occurrences are registered thus allowing relationships to be established between data acquisition, TJ-II control-system and diagnostic control-system actions. In the new database, raw data are stored in files on the TJ-II UNIX central server disks while meta-data are stored in Oracle tables thereby permitting fast data searches according to different criteria. In addition, libraries for registering data/events in the database from different subsystems within the laboratory local area network have been developed. Finally, a Shared Data Access System has been implemented for external access to data. It permits both new event-indexed as well as old data (indexed by shot number) to be read from a common event perspective.

  1. Development of enzyme-linked immunosorbent assay (ELISA) for glutathione S-transferase (GST-S) protein in the intertidal copepod Tigriopus japonicus and its application for environmental monitoring.

    Science.gov (United States)

    Rhee, Jae-Sung; Kim, Bo-Mi; Jeong, Chang-Bum; Leung, Kenneth Mei Yee; Park, Gyung Soo; Lee, Jae-Seong

    2013-11-01

    To utilize the GST-S protein as a useful biomarker for environmental contamination, we developed a polyclonal antibody-based enzyme-linked immunosorbent assay (ELISA) in the intertidal copepod Tigriopus japonicus. Two polyclonal antibodies, TJ-GST-S1 and TJ-GST-S2, were raised against two TJ-GST-S synthetic peptides. Also a recombinant TJ-GST-S protein was purified as a standard for ELISA development. Each polyclonal antibody was tested by Western blot analysis and indirect ELISA. Of two polyclonal antibodies, TJ-GST-S2 ELISA was further employed due to its wide range of detection and the limit of specificity compared to those of TJ-GST-S1 ELISA system. After exposure to 4 metals (Ag, As, Cd, and Cu) to T. japonicus, the amount of TJ-GST-S protein was significantly elevated in a concentration-dependent manner. Also, TJ-GST-S protein was upregulated at relative high concentrations of B[α]P, PCB, and TBT. In this paper, we suggest that T. japonicas ELISA for TJ-GST-S2 is useful as a potential indicator system for marine contaminants. Copyright © 2013 Elsevier Ltd. All rights reserved.

  2. TRPA1-dependent reversible opening of tight junction by natural compounds with an α,β-unsaturated moiety and capsaicin.

    Science.gov (United States)

    Kanda, Yusuke; Yamasaki, Youhei; Sasaki-Yamaguchi, Yoshie; Ida-Koga, Noriko; Kamisuki, Shinji; Sugawara, Fumio; Nagumo, Yoko; Usui, Takeo

    2018-02-02

    The delivery of hydrophilic macromolecules runs into difficulties such as penetration of the cell membrane lipid bilayer. Our prior experiment demonstrated that capsaicin induces the reversible opening of tight junctions (TJs) and enhances the delivery of hydrophilic macromolecules through a paracellular route. Herein, we screened paracellular permeability enhancers other than capsaicin. As TJ opening by capsaicin is associated with Ca 2+ influx, we first screened the compounds that induce Ca 2+ influx in layered MDCK II cells, and then we determined the compounds' abilities to open TJs. Our results identified several natural compounds with α,β-unsaturated moiety. A structure-activity relationship (SAR) analysis and the results of pretreatment with reducing reagent DTT suggested the importance of α,β-unsaturated moiety. We also examined the underlying mechanisms, and our findings suggest that the actin reorganization seen in capsaicin treatment is important for the reversibility of TJ opening. Furthermore, our analyses revealed that TRPA1 is involved in the Ca 2+ influx and TJ permeability increase not only by an α,β-unsaturated compound but also by capsaicin. Our results indicate that the α,β-unsaturated moiety can be a potent pharmacophore for TJ opening.

  3. Lecithin-Bound Iodine Prevents Disruption of Tight Junctions of Retinal Pigment Epithelial Cells under Hypoxic Stress

    Directory of Open Access Journals (Sweden)

    Masahiko Sugimoto

    2016-01-01

    Full Text Available Aim. We investigated whether lecithin-bound iodine (LBI can protect the integrity of tight junctions of retinal pigment epithelial cells from hypoxia. Method. Cultured human retinal pigment epithelial (ARPE-19 cells were pretreated with LBI. To mimic hypoxic conditions, cells were incubated with CoCl2. We compared the integrity of the tight junctions (TJs of control to cells with either LBI alone, CoCl2 alone, or LBI + CoCl2. The levels of cytokines in the conditioned media were also determined. Results. Significant decrease in the zonula occludens-1 (ZO-1 intensity in the CoCl2 group compared to the control (5787.7 ± 4126.4 in CoCl2 group versus 29244.6 ± 2981.2 in control; average ± standard deviation. But the decrease was not significant in the LBI + CoCl2 (27189.0 ± 11231.1. The levels of monocyte chemoattractant protein-1 (MCP-1 and Chemokine (C-C Motif Ligand 11 (CCL-11 were significantly higher in the CoCl2 than in the control (340.8 ± 43.3 versus 279.7 ± 68.3 pg/mL for MCP-1, and 15.2 ± 12.9 versus 12.5 ± 6.1 pg/mL for CCL-11. With LBI pretreatment, the levels of both cytokines were decreased to 182.6 ± 23.8 (MCP-1 and 5.46 ± 1.9 pg/mL for CCL-11. Blockade of MCP-1 or CCL-11 also shows similar result representing TJ protection from hypoxic stress. Conclusions. LBI results in a protective action from hypoxia.

  4. Josephson junction arrays

    International Nuclear Information System (INIS)

    Bindslev Hansen, J.; Lindelof, P.E.

    1985-01-01

    In this review we intend to cover recent work involving arrays of Josephson junctions. The work on such arrays falls naturally into three main areas of interest: 1. Technical applications of Josephson junction arrays for high-frequency devices. 2. Experimental studies of 2-D model systems (Kosterlitz-Thouless phase transition, commensurate-incommensurate transition in frustrated (flux) lattices). 3. Investigations of phenomena associated with non-equilibrium superconductivity in and around Josephson junctions (with high current density). (orig./BUD)

  5. Indications of Hydrocarbons in the Tjörnes Basin, North Iceland

    Science.gov (United States)

    Richter, B.; Brandsdóttir, B.; Detrick, R.; Helgadóttir, G.; Kjartansson, E.; Gunnarsson, K.; Driscoll, N.; Kent, G.

    2002-12-01

    The Tjörnes basin, located within the Tjörnes Fracture Zone (TFZ) was initiated during the Miocene (7-9 Ma), following an eastward jump of the spreading axis in N-Iceland. The roughly 150 km long (EW) and 50 km wide (NS) basin has since accumulated a 0.5-4 km thick sedimentary sequence within three extensional grabens, Eyjafjardaráll, Skjálfandadjúp and Öxarfjördur. The transtensional Húsavík-Flatey fault defines the southern margin of the basin. The hangingwalls within the westernmost and deepest graben (Eyjafjardaráll) are transected by series of synthetic and antithetic listric faults. These structures are affiliated with a crustal-scale, listric east- to north-easterly dipping master fault which soles out in the lower crust, at about 7500 m depth. Near shore sediments are exposed on the Tjörnes peninsula, at the eastern margin of the central graben, where a basal unit of Tertiary lava flows, up to 10 Ma old is overlain by 500 m thick Miocene-Pliocene sediments (Tjörnes Beds). Several lignite layers are present within this predominantly marine succession. Multichannel Seismic data show that the Tjörnes Beds extend westwards into the Skjálfandi Bay. In 1989, gas emissions of thermogenic hydrocarbons were detected during a core-drilling in the easternmost basin, Öxarfjördur. Analyses of natural gas-emissions within the geothermal areas at Skógarlón and Skógar in Öxarfjördur also revealed hydrocarbons (methane-hexane) in high concentrations. These gasses probably originate from marine sediments and lignites similar to those found in the Tjörnes Beds. The Tjörnes Beds, as seen in the Tjörnes horst, are thermally immature with respect to petroleum generation. However, based on the tectonic history, this is probably a minimum-maturity indication as organic geochemical analysis and maturation simulations indicate that high geothermal gradient enhances the formation of dry and wet gasses and waxy oil. In 2001 a sidescan sonar imaging was

  6. Equivalent Josephson junctions

    International Nuclear Information System (INIS)

    Boyadzhiev, T.L.; ); Semerdzhieva, E.G.; Shukrinov, Yu.M.; Fiziko-Tekhnicheskij Inst., Dushanbe

    2008-01-01

    The magnetic field dependences of critical current are numerically constructed for a long Josephson junction with a shunt- or resistor-type microscopic inhomogeneities and compared to the critical curve of a junction with exponentially varying width. The numerical results show that it is possible to replace the distributed inhomogeneity of a long Josephson junction by an inhomogeneity localized at one of its ends, which has certain technological advantages. It is also shown that the critical curves of junctions with exponentially varying width and inhomogeneities localized at the ends are unaffected by the mixed fluxon-antifluxon distributions of the magnetic flux [ru

  7. Study on Effect of Junction Temperature Swing Duration on Lifetime of Transfer Molded Power IGBT Modules

    DEFF Research Database (Denmark)

    Choi, Uimin; Blaabjerg, Frede; Jørgensen, Søren

    2017-01-01

    levels are presented. This study enables to include the tΔTj effect on lifetime model of IGBT modules for its lifetime estimation and it may result in improved lifetime prediction of IGBT modules under given mission profiles of converters. A postfailure analysis of the tested IGBT modules is also......In this paper, the effect of junction temperature swing duration on lifetime of transfer molded power insulated gate bipolar transistor (IGBT) modules is studied and a relevant lifetime factor is modeled. This study is based on 39 accelerated power cycling test results under six different...

  8. Surfing the wave, cycle, life history, and genes/proteins expressed by testicular germ cells. Part 5: intercellular junctions and contacts between germs cells and Sertoli cells and their regulatory interactions, testicular cholesterol, and genes/proteins associated with more than one germ cell generation.

    Science.gov (United States)

    Hermo, Louis; Pelletier, R-Marc; Cyr, Daniel G; Smith, Charles E

    2010-04-01

    In the testis, cell adhesion and junctional molecules permit specific interactions and intracellular communication between germ and Sertoli cells and apposed Sertoli cells. Among the many adhesion family of proteins, NCAM, nectin and nectin-like, catenins, and cadherens will be discussed, along with gap junctions between germ and Sertoli cells and the many members of the connexin family. The blood-testis barrier separates the haploid spermatids from blood borne elements. In the barrier, the intercellular junctions consist of many proteins such as occludin, tricellulin, and claudins. Changes in the expression of cell adhesion molecules are also an essential part of the mechanism that allows germ cells to move from the basal compartment of the seminiferous tubule to the adluminal compartment thus crossing the blood-testis barrier and well-defined proteins have been shown to assist in this process. Several structural components show interactions between germ cells to Sertoli cells such as the ectoplasmic specialization which are more closely related to Sertoli cells and tubulobulbar complexes that are processes of elongating spermatids embedded into Sertoli cells. Germ cells also modify several Sertoli functions and this also appears to be the case for residual bodies. Cholesterol plays a significant role during spermatogenesis and is essential for germ cell development. Lastly, we list genes/proteins that are expressed not only in any one specific generation of germ cells but across more than one generation. Copyright 2009 Wiley-Liss, Inc.

  9. Supramolecular tunneling junctions

    NARCIS (Netherlands)

    Wimbush, K.S.

    2012-01-01

    In this study a variety of supramolecular tunneling junctions were created. The basis of these junctions was a self-assembled monolayer of heptathioether functionalized ß-cyclodextrin (ßCD) formed on an ultra-flat Au surface, i.e., the bottom electrode. This gave a well-defined hexagonally packed

  10. TRPV4 Regulates Tight Junctions and Affects Differentiation in a Cell Culture Model of the Corneal Epithelium.

    Science.gov (United States)

    Martínez-Rendón, Jacqueline; Sánchez-Guzmán, Erika; Rueda, Angélica; González, James; Gulias-Cañizo, Rosario; Aquino-Jarquín, Guillermo; Castro-Muñozledo, Federico; García-Villegas, Refugio

    2017-07-01

    TRPV4 (transient receptor potential vanilloid 4) is a cation channel activated by hypotonicity, moderate heat, or shear stress. We describe the expression of TRPV4 during the differentiation of a corneal epithelial cell model, RCE1(5T5) cells. TRPV4 is a late differentiation feature that is concentrated in the apical membrane of the outmost cell layer of the stratified epithelia. Ca 2+ imaging experiments showed that TRPV4 activation with GSK1016790A produced an influx of calcium that was blunted by the specific TRPV4 blocker RN-1734. We analyzed the involvement of TRPV4 in RCE1(5T5) epithelial differentiation by measuring the development of transepithelial electrical resistance (TER) as an indicator of the tight junction (TJ) assembly. We showed that TRPV4 activity was necessary to establish the TJ. In differentiated epithelia, activation of TRPV4 increases the TER and the accumulation of claudin-4 in cell-cell contacts. Epidermal Growth Factor (EGF) up-regulates the TER of corneal epithelial cultures, and we show here that TRPV4 activation mimicked this EGF effect. Conversely, TRPV4 inhibition or knock down by specific shRNA prevented the increase in TER. Moreover, TRPP2, an EGF-activated channel that forms heteromeric complexes with TRPV4, is also concentrated in the outmost cell layer of differentiated RCE1(5T5) sheets. This suggests that the EGF regulation of the TJ may involve a heterotetrameric TRPV4-TRPP2 channel. These results demonstrated TRPV4 activity was necessary for the correct establishment of TJ in corneal epithelia and as well as the regulation of both the barrier function of TJ and its ability to respond to EGF. J. Cell. Physiol. 232: 1794-1807, 2017. © 2016 Wiley Periodicals, Inc. © 2016 Wiley Periodicals, Inc.

  11. Determination of the electronic temperature in the torsatron TJ-I Upgrade by the two filters

    Energy Technology Data Exchange (ETDEWEB)

    Medina, F.; Ochando, M.

    1994-07-01

    A Te monitor for the TJ-IU torsatron, based on the two-filters method, has been designed. It will consist of two surface-barrier silicon detectors looking at the same plasma region through berylium filters of different thickness. Plasma electron temperature is deduced from the ratio of the soft-x-ray fluxes transmitted through the two filters. The flexibility in magnetic configuration of TJ-IU plasmas has been taken into account in the mechanical design of this diagnostic. It will be attached to an upper 1 port of the vacuum vessel and the whole system will be movable both, to change the spatial resolution when needed and to enable the scan of the full plasma cross-section to obtain the radial profile of electron temperature in a shot-to-shot basis. (Author) 7 refs.

  12. Determination of the electronic temperature in the torsatron TJ-I Upgrade by the two filters

    International Nuclear Information System (INIS)

    Medina, F.; Ochando, M.

    1994-01-01

    A Te monitor for the TJ-IU torsatron, based on the two-filters method, has been designed. It will consist of two surface-barrier silicon detectors looking at the same plasma region through berylium filters of different thickness. Plasma electron temperature is deduced from the ratio of the soft-x-ray fluxes transmitted through the two filters. The flexibility in magnetic configuration of TJ-IU plasmas has been taken into account in the mechanical design of this diagnostic. It will be attached to an upper 1 port of the vacuum vessel and the whole system will be movable both, to change the spatial resolution when needed and to enable the scan of the full plasma cross-section to obtain the radial profile of electron temperature in a shot-to-shot basis. (Author) 7 refs

  13. Charge-Exchange Neutral Particle Analyzer Diagnostic of TJ-II

    International Nuclear Information System (INIS)

    Fontdecaba, J.M.; Balbin, R.; Petrov, S.; TJ-II team

    2003-01-01

    A description of the Charge Exchange Neutral Particle Analyzers in operation in the heliac flexible TJ-II is reported. A description of the detectors, as well as the operation characteristics, hardware and software used in the control and analysis of the data obtained with the diagnostic is detailed. Two NPAs are in operation in TJ-II. One of them is a 5-channel analyzer and another one is an Acord-12. The 5-channel analyzer provides measurements of charge exchange neutral fluxes at five energy channels, whereas the Acord-12 can measure simultaneously two different hydrogen isotopes (H and D) at six energy channels. Their lines of sight can be varied poloidally in order to observe the different sections of the plasma. (Author) 10 refs

  14. A method for measuring plasma position in the rectangular tokamak TJ-I

    International Nuclear Information System (INIS)

    Qin, J.; Ascasibar, E.; Pastor, I.; Navarro, A.P.; Ochando, M.A.; Pedrosa, M.A.; Rodriguez, L.; Sanchez, J.

    1993-01-01

    Determinations of plasma position in tokamaks are based on measuring the external magnetic field of the plasma current. Usually, the measurements are performed with magnetic coils along a closed contour around the plasma column, or with small magnetic probes in pairs positioned diametrically. However, such measurements are constrained in TJ-I because of a severe restriction on installing the magnetic probes. It is only possible to install some small magnetic probes inside the vacuum vessel near its top and bottom walls where the limiters stand. In this paper an alternative method of measuring plasma position in TJ-I is presented. The plasma position can be derived from the measurements of those magnetic probes located off the equatorial plane, with the aid of a filament model. The method is proved to be reliable, and would be applied to those small-size devices with similar restrictions. (author) 5 refs., 3 figs., 1 tab

  15. Determination of the electronic temperature in the torsatron TJ-I Upgrade by the two filters

    International Nuclear Information System (INIS)

    Medina, F.; Ochando, M.

    1994-01-01

    A Te monitor for the TJ-IU torsatron, based on the two-filters method, has been designed. It will consist of two surface-barrier silicon detectors looking at the same plasma region through berilium filters of different thickness. Plasma electron temperature is deduced from the ratio of the soft-x-ray fluxes transmitted through the two filters. The flexibility in magnetic configuration of TJ-IU plasmas has been taken into account in the mechanical design of this diagnostic. It will be attached to an upper port of the vacuum vessel and the whole system will be movable both, to change the spatial resolution when needed and to enable the scan of the full plasma cross-section to obtain the radial profile of electron temperature in a shot-to-shot basis. (Author)

  16. Influence of Rotational Transform and Magnetic Shear on the Energy Content of TJ-II Plasmas

    International Nuclear Information System (INIS)

    Estrada, T.; Ascasibar, E.; Castejon, F.; Jimenez, J. A.; Lopez-Bruna, D.; Pastor, I.

    2002-01-01

    In the magnetic configuration scans performed in TJ-II stellarator, low plasma energy content is found to be related to the presence of low order rational surfaces within the confinement region in low plasma density experiments. Plasma currents of about-1 kA (mainly bootstrap driven) can substantially increase the magnetic shear in TJ-II and under these conditions the confinement is no longer deteriorated by low order rational surfaces. Experiments with higher plasma currents (OH induced currents up to +/-10 kA) show a non-symmetric dependence on the sign of the magnetic shear. Preliminary results show a substantial improvement of the confinement in the case of negative plasma current, while minor changes are observed in the plasma energy content when positive current is induced in magnetic configurations that in vacuum exclude low order rational surfaces. (Author) 12 refs

  17. Calculation of the magnetic vector potential in the TJ-II

    International Nuclear Information System (INIS)

    Lopez Fraguas, A.; Lopez Bruna, D.; Romero, J. A.

    2005-01-01

    The properties of the vector magnetic potential and its usefulness to calculate magnetic fluxes in both stationary and time-dependent conditions are p revised in this report. We have adapted to the TJ-II Flexible Heliac efficient numerical expressions to calculate the vector potential, calculating in addition the magnetic flux with this formalism in circumstances whose complexity makes very convenient the use of the vector potential. The result on induced voltages offer theoretical support to the measurements of induced voltage due to the OH coils in the plasma, like the measurements provided by the loop voltage diagnostic installed in the TJ-II, as well as to the cylindrical approximation of the plasma often used to interpret experimental data. (Author) 11 refs

  18. Thermal loads on the TJ-II Vacuum Vessel under Neutral Beam Injection

    International Nuclear Information System (INIS)

    Guasp, J.; Fuentes, C.; Liniers, M.

    1996-01-01

    In this study a numerical analysis of power loads on the complex 3D structure of the TJ-II Vacuum Vessel, moderated with reasonable accuracy, under NBI, is done. To do this it has been necessary to modify deeply the DENSB code for power loads in order to include the TJ-II VV wall parts as targets and as beam scrapers, allowing the possibility of self-shadowing. After a short description of the primitive version of the DENSB code (paragraph 2) and of the visualisation code MOVIE(paragraph 3), the DENSB upgrading are described (paragraphs 4,5) and finally the results are presented (paragraph 6). These code modifications and the improving on the visualization tools provide more realistic load evaluations, both with and without plasma, validating former results and showing clearly the VV zones that will need new protections. (Author)

  19. Charge-Exchange Neutral Particle Analyzer Diagnostic of TJ-II

    Energy Technology Data Exchange (ETDEWEB)

    Fontdecaba, J. M.; Balbin, R.; Petrov, S.; TJ-II team

    2003-07-01

    A description of the Charge Exchange Neutral Particle Analyzers in operation in the heliac flexible TJ-II is reported. A description of the detectors, as well as the operation characteristics, hardware and software used in the control and analysis of the data obtained with the diagnostic is detailed. Two NPAs are in operation in TJ-II. One of them is a 5-channel analyzer and another one is an Acord-12. The 5-channel analyzer provides measurements of charge exchange neutral fluxes at five energy channels, whereas the Acord-12 can measure simultaneously two different hydrogen isotopes (H and D) at six energy channels. Their lines of sight can be varied poloidally in order to observe the different sections of the plasma. (Author) 10 refs.

  20. Manufacturing of a high precision coil system for the Spanish Stellarator TJ-II

    International Nuclear Information System (INIS)

    Alonso, J.; Blaumoser, M.; Bieder, H.E.; Theisen, E.; Jeckle, A.; Brandl, M.; Mione, M.

    1995-01-01

    The flexible Heliac TJ-II is under construction at Ciemat in Madrid, Spain. This experimental device allows the investigation of plasmas in a wide range of magnetic field configurations. The rotational transform can be varied between 0.9 and 2.5. Shear variation is possible from -1% to 10%. The magnetic well ranges from 0 to 6%. The average major plasma radius is 1.5 m and the minor plasma dimensions are 0.4 m by 0.2 m. The average toroidal magnetic field is 1 T. The central part of TJ-II is a coil system called Hard Core. The manufacturing aspects of this component are described hereafter. The narrow range of tolerances of the different elements is consequence of the necessary precision of the coils that are located very close to the plasma. (orig.)

  1. Influence of Rotational Transform and Magnetic Shear on the Energy Content of TJ-II Plasmas

    Energy Technology Data Exchange (ETDEWEB)

    Estrada, T.; Ascasibar, E.; Castejon, F.; Jimenez, J. A.; Lopez-Bruna, D.; Pastor, I.

    2002-07-01

    In the magnetic configuration scans performed in TJ-II stellarator, low plasma energy content is found to be related to the presence of low order rational surfaces within the confinement region in low plasma density experiments. Plasma currents of about-1 kA (mainly bootstrap driven) can substantially increase the magnetic shear in TJ-II and under these conditions the confinement is no longer deteriorated by low order rational surfaces. Experiments with higher plasma currents (OH induced currents up to +/-10 kA) show a non-symmetric dependence on the sign of the magnetic shear. Preliminary results show a substantial improvement of the confinement in the case of negative plasma current, while minor changes are observed in the plasma energy content when positive current is induced in magnetic configurations that in vacuum exclude low order rational surfaces. (Author) 12 refs.

  2. Broad-band time-resolved near infrared spectroscopy in the TJ-II stellarator

    Energy Technology Data Exchange (ETDEWEB)

    Rodriguez, M.C.; Pastor, I.; Cal, E. de la; McCarthy, K.J. [Laboratorio Nacional de Fusion, CIEMAT, Madrid (Spain); Diaz, D. [Universidad Autonoma de Madrid, Dept Quimica Fisica Aplicada, Madrid (Spain)

    2014-11-15

    First experimental results on broad-band, time-resolved Near Infrared (NIR;here loosely defined as covering from 750 to 1650 nm) passive spectroscopy using a high sensitivity InGaAs detector are reported for the TJ-II Stellarator. Experimental set-up is described together with its main characteristics, the most remarkable ones being its enhanced NIR response, broadband spectrum acquisition in a single shot, and time-resolved measurements with up to 1.8 kHz spectral rate. Prospects for future work and more extended physics studies in this newly open spectral region in TJ-II are discussed. (copyright 2014 WILEY-VCH Verlag GmbH and Co. KGaA, Weinheim) (orig.)

  3. Joint diseases: from connexins to gap junctions.

    Science.gov (United States)

    Donahue, Henry J; Qu, Roy W; Genetos, Damian C

    2017-12-19

    Connexons form the basis of hemichannels and gap junctions. They are composed of six tetraspan proteins called connexins. Connexons can function as individual hemichannels, releasing cytosolic factors (such as ATP) into the pericellular environment. Alternatively, two hemichannel connexons from neighbouring cells can come together to form gap junctions, membrane-spanning channels that facilitate cell-cell communication by enabling signalling molecules of approximately 1 kDa to pass from one cell to an adjacent cell. Connexins are expressed in joint tissues including bone, cartilage, skeletal muscle and the synovium. Indicative of their importance as gap junction components, connexins are also known as gap junction proteins, but individual connexin proteins are gaining recognition for their channel-independent roles, which include scaffolding and signalling functions. Considerable evidence indicates that connexons contribute to the function of bone and muscle, but less is known about the function of connexons in other joint tissues. However, the implication that connexins and gap junctional channels might be involved in joint disease, including age-related bone loss, osteoarthritis and rheumatoid arthritis, emphasizes the need for further research into these areas and highlights the therapeutic potential of connexins.

  4. Impurity Dynamics under Neutral Beam Injection at TJ-II (simulation); Dinamica de Impurezas durante la Inyeccion de Haces Neutros en el TJ-II (simulacion)

    Energy Technology Data Exchange (ETDEWEB)

    Guasp, J.; Fuentes, C.; Liniers, M.

    2001-07-01

    In this study the simulations of plasma transport under NBI for TJ-II, previously performed, are extended. Since than a considerable number of important modifications have been introduced in the model: change of magnetic configuration, use of experimental initial profiles, expansion of the Data base from NBI calculations and, mainly, a detailed handling of impurities with inclusion of sputtering effects. Moreover there is now a particular emphasis on the analysis of the conditions for discharge collapse and on the possible effects of single beam injection. This analysis of impurity behaviour with sputtering shows that in the expected usual cases there is no radioactive collapse and that if the recycling coefficients remain lower the unity it is always possible to find a strategy for external gas puffing leading to a stationary state, with densities below the limit and efficient NBI absorption (>50%). The radioactive collapse can appear either at high densities (central value higher than 1.4x10''20 m''3), excessive influx of impurities (i. e. with sputtering rates higher than twice the expected values) o for insufficient injected beam power (less than 45 kW). The present study analyses only the 100{sub 4}4{sub 6}4 configuration of TJ-II, but future works will start a systematic scan of configuration using this same model. (Author) 12 Refs.

  5. Transmission of the Neutral Beam Heating Beams at TJ-II; Transmision del Haz de Neutros de Calentamiento en TJ-II

    Energy Technology Data Exchange (ETDEWEB)

    Fuentes Lopez, C

    2007-09-27

    Neutral beam injection heating has been development for the TJ-II stellarator. The beam has a port-through power between 700-1500 kW and injection energy 40 keV. The sensibility of the injection system to the changes of several parameters is analysed. Beam transmission is limited by losses processes since beam is born into the ions source until is coming into the fusion machine. For the beam transmission optimization several beam diagnostics have been developed. A carbon fiber composite (CFC) target calorimeter has been installed at TJ-II to study in situ the power density distribution of the neutral beams. The thermographic print of the beam can be recorded and analysed in a reliable way due to the highly anisotropic thermal conductivity of the target material. With the combined thermographic and calorimetric measurements it has been possible to determine the power density distribution of the beam. It has been found that a large beam halo is present, which can be explained by the extreme misalignment of the grids. This kind of halo has a deleterious effect on beam transport and must be minimized in order to improve the plasma heating capability of the beams. (Author) 155 refs.

  6. Power supply for the Spanish stellarator TJ-II, design, construction, and tests

    Energy Technology Data Exchange (ETDEWEB)

    Perez, A.; Lucia, C.; Alberdi, B.; Del Rio, J.M. [JEMA SA, Lasarte-Oria (Spain); Almoguera, L.; Blaumoser, M.; Kirpitchev, I.; Mendez, P. [Asociacion EURATOM-CIEMAT para Fusion, Madrid (Spain)

    1995-12-31

    Most of the components of the electrical power supply system of the new TJ-II stellarator, which is under construction in Madrid (Spain), are now constructed and tested. The flywheel synchronous generator is still under construction and its tests are planned for the end of 1995. The power plant is described in detail as well as the tests which have been carried out and their results.

  7. The Mobile Limiters of TJ-II: Power and Particle Control

    International Nuclear Information System (INIS)

    Cal, E. de la

    1998-01-01

    For mobile limiters have been designed for the TJ-II stellerator to reduce thermal loads on the vacuum vessel and its protections at the region of the central hard core (groove) and to characterise the scrap off layer plasma. The role of the mobile limiters for particle and thermal load control is analysed for the different operating phases of TJ-II. The task of impurity control will be treated in a future report. A simplified model has been used to estimate the termal loads on the limiters. The conclusion is that a new design for the limiter heads will be necessary for the neutral beam injection (NBI)-phase at high power density, if acceptable efficiencies of thermal removal is desired. The rexperimental measurements which will be made in the first phase (ECH) with the temperature and Lagmuir probes installed in the diagnosed limiter-heads will be essential for the optimisation of the future limiter-shape. For particle control it will be absolutely necessary to use first wall conditioning techniques (e.g. boronization), since no active pumping method is foreseen for TJ-II. Again, this point will be more critical in the NBI-phase, due to the large particle fluxes to the first wall and due to possible thermal gas, desorption caused by local overheating of plasma-facing surfaces. The role of magnetic topology on plasma-wall interaction is finally analysed. A configuration has been found in which the limiters act as divertor plates (Natural Island Divertor). This inherent flexibility for changing the magnetic topology of TJ-II should be exploited in order to find the most favourable operating scenarios for the high powder injection phase

  8. On the Role of Impurity Radiation on Edge Turbulence in the TJ-I Tokamak

    Energy Technology Data Exchange (ETDEWEB)

    Ochando, M A; Pedrosa, M A; Balbin, R; Garcia-Cortes, I; Hidalgo, C

    1994-07-01

    The correlation between edge radiation and electron temperature and density fluctuations has been studied in the vicinity of the upper poloidal limiter of the TJ-I tokamak. When edge impurity radiation is strongly peaked in the proximity of the limiter radius, electron temperature fluctuations are notably higher than density fluctuations. Results provide experimental evidence of edge turbulence driven by thermal instabilities. (Author) 16 refs.

  9. A method for measuring plasma position in TJ-I Tokamak

    International Nuclear Information System (INIS)

    Quin, J.; TJ-I, Team

    1993-01-01

    A method using pairs of Mirnov coils to measure the plasma position in TJ-I is presented. The simple toroidal filament model which neglects the effect of plasma current density profile has proven to be acceptable within the experimental accuracy. The effect of plasma current density profile remains to be small, if the plasma current density profile has a quadratic form. (Author) 5 refs

  10. On the Role of Impurity Radiation on Edge Turbulence in the TJ-I Tokamak

    International Nuclear Information System (INIS)

    Ochando, M. A.; Pedrosa, M. A.; Balbin, R.; Garcia-Cortes, I.; Hidalgo, C.

    1994-01-01

    The correlation between edge radiation and electron temperature and density fluctuations has been studied in the vicinity of the upper poloidal limiter of the TJ-I tokamak. When edge impurity radiation is strongly peaked in the proximity of the limiter radius, electron temperature fluctuations are notably higher than density fluctuations. Results provide experimental evidence of edge turbulence driven by thermal instabilities. (Author) 16 refs

  11. A method for measuring plasma position in TJ-I Tokamak

    Energy Technology Data Exchange (ETDEWEB)

    Quin, J.; TJ-I, Team

    1993-07-01

    A method using pairs of Mirnov coils to measure the plasma position in TJ-I is presented. The simple toroidal filament model which neglects the effect of plasma current density profile has proven to be acceptable within the experimental accuracy. The effect of plasma current density profile remains to be small, if the plasma current density profile has a quadratic form. (Author) 5 refs.

  12. Experimental investigation of edge sheared flow development and configuration effects in the TJ-II stellarator

    International Nuclear Information System (INIS)

    Pedrosa, M.A.; Hidalgo, C.; Alonso, A.; Calderon, E.; Orozco, O.; Pablos, J.L. de

    2005-01-01

    Experimental results have shown that the generation of spontaneous perpendicular sheared flow (i.e. the naturally occurring shear layer) requires a minimum plasma density or gradient in the TJ-II stellarator. This finding has been observed by means of multiple plasma diagnostics, including probes, fast cameras, reflectometry and HIBP. The obtained shearing rate of the naturally occurring shear layer results in general comparable to the one observed during biasing-improved confinement regimes. It has been found that there is a coupling between the onset of sheared flow development and an increase in the level of plasma edge fluctuations pointing to turbulence as the main ingredient of the radial electric field drive; once the shear flow develops the level of turbulence tends to decrease. The link between the development of sheared flows and plasma density in TJ-II has been observed in different magnetic configurations and plasma regimes. Preliminary results show that the threshold density value depends on the iota value and on the magnetic ripple (plasma volume). Recent experiments carried out in the LHD stellarator have shown that edge sheared flows are also affected by the magnitude of edge magnetic ripple: the threshold density to trigger edge sheared flows increases with magnetic ripple . Those results have been interpreted as an evidence of the importance of neoclassical effect in the physics of ExB sheared flows. For some TJ-II magnetic configurations with higher edge iota (ι/2π≥ 1.8) there is a sharp increase in the edge density gradient simultaneous to a strong reduction of fluctuations and transport and a slight increase of the shearing rate and perpendicular rotation (≥2 km/s) as density increases above the threshold. The role of the edge ripple, the presence of edge rational surfaces and properties of turbulent transport are considered as possible ingredients to explain the spontaneous development of edge sheared flows in TJ-II. (author)

  13. Installation of the advanced heavy ion beam probing diagnostic on the TJ-II stellarator

    International Nuclear Information System (INIS)

    Bondarenko, I.S.; Chmyga, A.A.; Dreval, N.B.

    2000-01-01

    An advanced heavy ion beam diagnostic has been developed for the TJ-II stellarator based on the simultaneous utilisation of two different detection systems for the secondary ions: a multiple cell array detector and a 30 deg Proca-Green electrostatic energy analyser. This innovative design aims at enlarging the HIBD capabilities to allow the instantaneous measurements of electron density and plasma potential profiles together with their respective fluctuations. This paper presents the detailed description of the main parts of HIBD and their characteristics obtained during the first operation on TJ-II. Special attention is paid to the control and data acquisition system built on two VME controllers. The results of the diagnostic beam propagating through the magnetic structure of TJ-II into electrostatic energy analyser are presented and compared with the trajectory calculations. The operation and calibration of a 30 deg electrostatic energy analyser free of guard rings and with a new biased split detector are described. High intensities of the caesium and thallium ions were obtained from thermionic source using new stable and long-time special operation regimes. (author)

  14. Visual Data Analysis in the TJ-II Remote Participation System

    Energy Technology Data Exchange (ETDEWEB)

    Sanchez, E.; Porta, A.; Pereira, A.; Vega, J.

    2007-07-20

    A general-purpose data visualization tool has been developed to provide the TJ-II remote participation system with the same visualization capabilities already available in the TJ-II local environment. The visualization software has been developed in the Java language. It provides a user-friendly graphical interface that permits users on-demand plotting of time traces in a very flexible manner. In order to facilitate on-line tracking of experimental operation, the application also allows automatic refreshing of data. This software has been integrated into the TJ-II remote participation system distributed environment. Data are accessed remotely using web technologies and HTTP protocol and are transferred in a compressed format, which reduces bandwidth requirements. Both metadata and binary compressed data are transported in multi part messages. Message oriented middle ware software is used to distribute information on-line, in particular notifications of data availability for automatic data refreshing or local events. Plot layouts can be stored in a centralized database for subsequent recovery from anywhere. Finally, this software is integrated into the general security framework provided by the PAPI system. (Author) 16 refs.

  15. Dynamical Simulation of Recycling and Particle Fueling in TJ-II Plasmas

    International Nuclear Information System (INIS)

    Lopez-Bruna, D.; Ferreira, J. A.; Tabares, F. L.; Castejon, F.; Guasp, J.

    2007-01-01

    With the aim of improving the calculation tools for transport analysis in TJ-II plasmas, in this work we analyze the simplified model for a kinetic equation that ASTRA uses to calculate the neutral particle distribution in the plasma. Next, we act on the boundary conditions for this kinetic equation (particularly on the neutral density in the plasma boundary) so we can simulate the recycling conditions for the TJ-II in a simple way. With the resulting transport models we can easily analyze the sensibility of these plasmas to the cold gas puffing depending on the recycling conditions. These transport models evidence the problem of density control in the TJ-II. Likewise, we estimate the importance of recycling in the plasmas heated by energetic neutral beam injection. The experimentally observed increments in density when the energetic neutrals are injected would respond, according to the calculations here presented, to a large increment of the neutrals influx that cannot be explained by the beam itself. (Author) 22 refs

  16. Magnetic Configuration Effects Under Neutral Beam Injection at TJ-II

    International Nuclear Information System (INIS)

    Guasp, J.; Liniers, M.

    1998-01-01

    The theoretical analysis of NBI absorption and losses, done for the Reference configuration of TJ-II, has been extended to other magnetic configurations of the flexibility diagram. The main results obtained are the following: Fast ion losses. mainly direct ones, are the determinant factor the absorption behaviour. In the absence of radial electric field, the contribution of the delayed fast ion losses in minimal, as well with CX as without, and corresponds, almost exclusively, to low energy trapped ions (1 to t KeV). There is a strong difference between the direct los behaviour corresponding to both injection directions CO and COUNTER. The first one gives always higher losses in TJ-II. For the extreme configurations the direct losses are very high and are originated by resonant effects, that can be observed even for null electric field, and are due to the 0 and-2 resonances. The intermediate configurations are equally separated from both resonances, in consequence the loss level is lower, producing absorption ratios very, acceptable, higher than 60% of the power entering torus at high density and 40 keV. This corresponds to about 1.2 MW absorbed in plasma under balanced injection. In conclusion, the possible presence of resonant effects on the direct losses is the key element to explain the absorption behaviour for the different magnetic configurations. In addition all the configurations placed inside a wide region around the Reference case in the flexibility diagram seem equally convenient for NBI in TJ-II. (Author) 18 refs

  17. Visual Data Analysis in the TJ-II Remote Participation System

    International Nuclear Information System (INIS)

    Sanchez, E.; Porta, A.; Pereira, A.; Vega, J.

    2007-01-01

    A general-purpose data visualization tool has been developed to provide the TJ-II remote participation system with the same visualization capabilities already available in the TJ-II local environment. The visualization software has been developed in the Java language. It provides a user-friendly graphical interface that permits users on-demand plotting of time traces in a very flexible manner. In order to facilitate on-line tracking of experimental operation, the application also allows automatic refreshing of data. This software has been integrated into the TJ-II remote participation system distributed environment. Data are accessed remotely using web technologies and HTTP protocol and are transferred in a compressed format, which reduces bandwidth requirements. Both metadata and binary compressed data are transported in multi part messages. Message oriented middle ware software is used to distribute information on-line, in particular notifications of data availability for automatic data refreshing or local events. Plot layouts can be stored in a centralized database for subsequent recovery from anywhere. Finally, this software is integrated into the general security framework provided by the PAPI system. (Author) 16 refs

  18. Geometry of the TJ-II in Astra 6.0

    International Nuclear Information System (INIS)

    Lopez-Bruna, D.; Romero, J.A.; Castejon, F.

    2006-01-01

    One of the most exploited features of the TJ-II Heliac, a facility in the Laboratorio Nacional de Fusion (CIEMAT, Madrid), is its ability to explore plasmas in different magnetic configurations. For this reason, there are available libraries that provide the metrics and associated magnitudes for many among all possible configurations. On the other hand, the transport codes that can normally be used to perform transport calculations cannot dea properly with these geometries, which is especially delicate when there are induced plasma currents. In the present work we adopt ASTRA, a transport analysis shell, to study the approximations performed when calculations that impose axi-symmetry (as ASTRA does) are performed on magnetic configurations that are not really axi-symmetric. After describing how we obtain those TJ-II metric averages that must be set in ASTRA, we perform two comparisons: (i) we obtain the vacuum rotational transform as deduced from the metric coefficients but imposing axisymmetry, and compare the results with the rotational transform yielded by the existing libraries; and (ii) we build a ID transport code with TJ-II metrics so its results can be compared with those of ASTRA. In both cases, the differences found indicate that evaluating the evolution of the rotational transform under ohmic induction and transport evolution is acceptable assuming that the geometry itself does not evolve. (Author) 11 refs

  19. A relational database for physical data from TJ-II discharges

    International Nuclear Information System (INIS)

    Sanchez, E.; Portas, A.B.; Vega, J.

    2002-01-01

    A relational database (RDB) has been developed for classifying TJ-II experimental data according to physical criteria. Two objectives have been achieved: the design and the implementation of the database and the software tools for data access depending on a single software driver. TJ-II data were arranged in several tables with a flexible design, speedy performance, efficient search capacity and adaptability to meet present and future, requirements. The software has been developed to allow the access to the TJ-II RDB from a variety of computer platforms (ALPHA AXP/True64 UNIX, CRAY/UNICOS, Intel Linux, Sparc/Solaris and Intel/Windows 95/98/NT) and programming languages (FORTRAN and C/C++). The database resides in a Windows NT Server computer and is managed by Microsoft SQL Server. The access software is based on open network computing remote procedure call and follows client/server model. A server program running in the Windows NT computer controls data access. Operations on the database (through a local ODBC connection) are performed according to predefined permission protocols. A client library providing a set of basic functions for data integration and retrieval has been built in both static and dynamic link versions. The dynamic version is essential in accessing RDB data from 4GL environments (IDL and PV-WAVE among others)

  20. Visual Data Analysis in the TJ-II Remote Participation System

    International Nuclear Information System (INIS)

    Sanchez, E.; Portas, A.; Pereira, A.; Vega, J.

    2006-01-01

    A general-purpose data visualization tool has been developed to provide the TJ-II remote participation system with the same visualization capabilities already available in the TJ-II local environment. The visualization software has been developed in the Java language. It provides a user-friendly graphical interface that permits users on-demand plotting of time traces in a very flexible manner. In order to facilitate on-line tracking of experimental operation, the application also allows automatic refreshing of data. This software has been integrated into the TJ-II remote participation system distributed environment. Data are accessed remotely using web technologies and HTTP protocol and are transferred in a compressed format, which reduces bandwidth requirements. Both metadata and binary compressed data are transported in multipart messages. Message oriented middleware software is used to distribute information on-line, in particular notifications of data availability for automatic data refreshing or local events. Plot layouts can be stored in a centralized database for subsequent recovery from anywhere. Finally, this software is integrated into the general security framework provided by the PAPI system. (Author)

  1. Production of a High Efficiency Microbial Flocculant by Proteus mirabilis TJ-1 Using Compound Organic Wastewater

    Science.gov (United States)

    Zhang, Zhiqiang; Xia, Siqing; Zhang, Jiao

    2010-11-01

    The production of a high efficiency microbial flocculant (MBF) by Proteus mirabilis TJ-1 using compound organic wastewater was investigated. To cut down the cost of the MBF production, several nutritive organic wastewaters were selected to replace glucose and peptone as the carbon source and the nitrogen source in the optimized medium of strain TJ-1, respectively. The compound wastewater of the milk candy and the soybean milk was found to be good carbon source and nitrogen source for this strain to produce MBF. The cost-effective culture medium consists of (per liter): 800 mL wastewater of milk candy, 200 mL wastewater of soybean milk, 0.3 g MgSO4ṡ7 H2O, 5 g K2HPO4, 2 g and KH2PO4, pH 7.0. The economic cost for the MBF production can be cut down over a half by using the developed culture medium. Furthermore, the utilization of the two wastewaters in the preparation of culture medium of strain TJ-1 can not only save their big treatment cost, but also realize their resource reuse.

  2. Visual Data Analysis in the TJ-II Remote Participation System

    Energy Technology Data Exchange (ETDEWEB)

    Sanchez, E.; Portas, A.; Pereira, A.; Vega, J.

    2006-07-01

    A general-purpose data visualization tool has been developed to provide the TJ-II remote participation system with the same visualization capabilities already available in the TJ-II local environment. The visualization software has been developed in the Java language. It provides a user-friendly graphical interface that permits users on-demand plotting of time traces in a very flexible manner. In order to facilitate on-line tracking of experimental operation, the application also allows automatic refreshing of data. This software has been integrated into the TJ-II remote participation system distributed environment. Data are accessed remotely using web technologies and HTTP protocol and are transferred in a compressed format, which reduces bandwidth requirements. Both metadata and binary compressed data are transported in multipart messages. Message oriented middleware software is used to distribute information on-line, in particular notifications of data availability for automatic data refreshing or local events. Plot layouts can be stored in a centralized database for subsequent recovery from anywhere. Finally, this software is integrated into the general security framework provided by the PAPI system. (Author)

  3. Reduced E-cadherin expression is associated with abdominal pain and symptom duration in a study of alternating and diarrhea predominant IBS.

    LENUS (Irish Health Repository)

    Wilcz-Villega, E

    2013-11-29

    Increased intestinal permeability and altered expression of tight junction (TJ) proteins may be implicated in the pathogenesis of irritable bowel syndrome (IBS). This study aimed to investigate the expression of adherens junction (AJ) protein E-cadherin and TJ proteins zonula occludens (ZO)-1 and claudin (CLD)-1 and associations with IBS symptoms.

  4. ‘Gap Junctions and Cancer: Communicating for 50 Years’

    Science.gov (United States)

    Aasen, Trond; Mesnil, Marc; Naus, Christian C.; Lampe, Paul D.; Laird, Dale W.

    2017-01-01

    Fifty years ago, tumour cells were found to lack electrical coupling, leading to the hypothesis that loss of direct intercellular communication is commonly associated with cancer onset and progression. Subsequent studies linked this phenomenon to gap junctions composed of connexin proteins. While many studies support the notion that connexins are tumour suppressors, recent evidence suggests that, in some tumour types, they may facilitate specific stages of tumour progression through both junctional and non-junctional signalling pathways. This Timeline article highlights the milestones connecting gap junctions to cancer, and underscores important unanswered questions, controversies and therapeutic opportunities in the field. PMID:27782134

  5. β-Conglycinin Reduces the Tight Junction Occludin and ZO-1 Expression in IPEC-J2

    Directory of Open Access Journals (Sweden)

    Yuan Zhao

    2014-01-01

    Full Text Available Soybean allergy presents a health threat to humans and animals. The mechanism by which food/feed allergen β-conglycinin injures the intestinal barrier has not been well understood. In this study, the changes of epithelial permeability, integrity, metabolic activity, the tight junction (TJ distribution and expression induced by β-conglycinin were evaluated using IPEC-J2 model. The results showed a significant decrease of trans-epithelial electrical resistance (TEER (p < 0.001 and metabolic activity (p < 0.001 and a remarkable increase of alkaline phosphatase (AP activity (p < 0.001 in a dose-dependent manner. The expression levels of tight junction occludin and ZO-1 were decreased (p < 0.05. The reduced fluorescence of targets and change of cellular morphology were recorded. The tight junction occludin and ZO-1 mRNA expression linearly declined with increasing β-conglycinin (p < 0.001.

  6. Biochemical characterization of a heterotrimeric G(i)-protein activator peptide designed from the junction between the intracellular third loop and sixth transmembrane helix in the m4 muscarinic acetylcholine receptor.

    Science.gov (United States)

    Terawaki, Shin-ichi; Matsubayashi, Rina; Hara, Kanako; Onozuka, Tatsuki; Kohno, Toshiyuki; Wakamatsu, Kaori

    Muscarinic acetylcholine receptors (mAChRs) are G-protein coupled receptors (GPCRs) that are activated by acetylcholine released from parasympathetic nerves. The mAChR family comprises 5 subtypes, m1-m5, each of which has a different coupling selectivity for heterotrimeric GTP-binding proteins (G-proteins). m4 mAChR specifically activates the Gi/o family by enhancing the guanine nucleotide exchange factor (GEF) reaction with the Gα subunit through an interaction that occurs via intracellular segments. Here, we report that the m4 mAChR mimetic peptide m4i3c(14)Gly, comprising 14 residues in the junction between the intracellular third loop (i3c) and transmembrane helix VI (TM-VI) extended with a C-terminal glycine residue, presents GEF activity toward the Gi1 α subunit (Gαi1). The m4i3c(14)Gly forms a stable complex with guanine nucleotide-free Gαi1 via three residues in the VTI(L/F) motif, which is conserved within the m2/4 mAChRs. These results suggest that this m4 mAChR mimetic peptide, which comprises the amino acid of the mAChR intracellular segments, is a useful tool for understanding the interaction between GPCRs and G-proteins. Copyright © 2015 Elsevier Inc. All rights reserved.

  7. Aberrant expression of the tight junction molecules claudin-1 and zonula occludens-1 mediates cell growth and invasion in oral squamous cell carcinoma.

    Science.gov (United States)

    Babkair, Hamzah; Yamazaki, Manabu; Uddin, Md Shihab; Maruyama, Satoshi; Abé, Tatsuya; Essa, Ahmed; Sumita, Yoshimasa; Ahsan, Md Shahidul; Swelam, Wael; Cheng, Jun; Saku, Takashi

    2016-11-01

    We reported that altered cell contact mediated by E-cadherin is an initial event in the pathogenesis of oral epithelial malignancies. To assess other effects of cell adhesion, we examined the expression levels of tight junction (TJ) molecules in oral carcinoma in situ (CIS) and squamous cell carcinoma (SCC). To identify changes in the expression of TJ molecules, we conducted an analysis of the immunohistochemical profiles of claudin-1 (CLDN-1) and zonula occludens-1 (ZO-1) in surgical specimens acquired from patients with oral SCC containing foci of epithelial dysplasia or from patients with CIS. We used immunofluorescence, Western blotting, reverse-transcription polymerase chain reaction, and RNA interference to evaluate the functions of CLDN-1 and ZO-1 in cultured oral SCC cells. TJ molecules were not detected in normal oral epithelial tissues but were expressed in SCC/CIS cells. ZO-1 was localized within the nucleus of proliferating cells. When CLDN-1 expression was inhibited by transfecting cells with specific small interference RNAs, SCC cells dissociated, and their ability to proliferate and invade Matrigel was inhibited. In contrast, although RNA interference-mediated inhibition of ZO-1 expression did not affect cell morphology, it inhibited cell proliferation and invasiveness. Our findings indicated that the detection of TJ molecules in the oral epithelia may serve as a marker for the malignant phenotype of cells in which CLDN-1 regulates proliferation and invasion. Copyright © 2016 Elsevier Inc. All rights reserved.

  8. Origin of the Degradation of Triple Junction Solar Cells at low Temperature

    Directory of Open Access Journals (Sweden)

    Park Seonyong

    2017-01-01

    Full Text Available The degradation of solar cells under irradiation by high energy particles (electrons, protons is the consequence of the introduction of defects trapping minority carriers, which are then not collected by the junction. However, at low temperature, defects located in the space charge region can also induce a tunneling current that results in an apparent decreases of the maximum power. The degradation produced by this tunneling current can depend on temperature, since the concentration of defects created by an irradiation is usually temperature dependent, and can be larger than the degradation associated with carrier recombination. For instance, as we shall see below, an irradiation with 1 MeV electrons at 120 K with a fluence of 3.0 × 1015 /cm2 induces a decrease of less than 10 % in the short-circuit current (Isc and open-circuit voltage (Voc of triple junction (TJ cells, but a decrease of about 40 % in the maximum power (Pmax, which implies that more than half of the total degradation of Pmax should be assigned to another loss mechanism, tunneling in this case. In this work, we demonstrate that this additional degradation must indeed be ascribed to a tunneling process and we investigate the variation of the tunneling current versus fluence induced by electron irradiation in TJ cells, in order to tentatively ascribe the tunneling components to specific sub-cells.

  9. Primary Tunnel Junction Thermometry

    International Nuclear Information System (INIS)

    Pekola, Jukka P.; Holmqvist, Tommy; Meschke, Matthias

    2008-01-01

    We describe the concept and experimental demonstration of primary thermometry based on a four-probe measurement of a single tunnel junction embedded within four arrays of junctions. We show that in this configuration random sample specific and environment-related errors can be avoided. This method relates temperature directly to Boltzmann constant, which will form the basis of the definition of temperature and realization of official temperature scales in the future

  10. Spectroscopic system for impurity measurements in the TJ-1 Tokamak of JEN; Un sistema espectroscopico para medidas de impurezas en el Tokamak TJ-1 de la JEN

    Energy Technology Data Exchange (ETDEWEB)

    Navas, G; Zurro, B

    1982-07-01

    we describe a spectroscopic system with spatial resolution capability that has been configured for plasma diagnostic in the TJ-1 Tokamak of JEN. The experimental system, based on a one meter monochromator, has been absolutely calibrated using a tungsten-halogen lamp. The calibration procedures and the absolute spectral sensitivity are presented as well as its dependence with the polarization. A simplified spectroscopic model of the radiation emitted by the intrinsic plasma impurities (C, 0, . . . ) has been developed. A one dimensional model of the temporal evolution of various ionization stages in coronal equilibrium is used to predict the electron temperature and impurity concentration. This model has been applied to experimental data from several Tokamaks. (Author) 23 refs.

  11. Conditioning of TJ-II Stellarator during the ECRH Plasmas Period; Acondicionamiento del Stellarator TJ-II durante la Etapa de Plasmas ECRH

    Energy Technology Data Exchange (ETDEWEB)

    Tafalla, D.; Tabares, F.L.

    2001-07-01

    The TJ-II stellarator has been conditioned by glow discharge (GD) during the first campaigns of operation, working only with ECR heating and all metal walls. The application of a He GD during the overnight period before the operation has been required in order to obtain reproducible discharges. However, the density control of the ECRH discharges was not possible because of the He implanted on the wall during GS. An short Ar GD({<=}30 min) applied before the operation allows desorbes part of the implanted He. By applying this procedure (HeGD+ArGD), reproducible and density controlled plasmas have been achieved in H{sub 2} and He. (Author) 20 refs.

  12. Quantum Junction Solar Cells

    KAUST Repository

    Tang, Jiang

    2012-09-12

    Colloidal quantum dot solids combine convenient solution-processing with quantum size effect tuning, offering avenues to high-efficiency multijunction cells based on a single materials synthesis and processing platform. The highest-performing colloidal quantum dot rectifying devices reported to date have relied on a junction between a quantum-tuned absorber and a bulk material (e.g., TiO 2); however, quantum tuning of the absorber then requires complete redesign of the bulk acceptor, compromising the benefits of facile quantum tuning. Here we report rectifying junctions constructed entirely using inherently band-aligned quantum-tuned materials. Realizing these quantum junction diodes relied upon the creation of an n-type quantum dot solid having a clean bandgap. We combine stable, chemically compatible, high-performance n-type and p-type materials to create the first quantum junction solar cells. We present a family of photovoltaic devices having widely tuned bandgaps of 0.6-1.6 eV that excel where conventional quantum-to-bulk devices fail to perform. Devices having optimal single-junction bandgaps exhibit certified AM1.5 solar power conversion efficiencies of 5.4%. Control over doping in quantum solids, and the successful integration of these materials to form stable quantum junctions, offers a powerful new degree of freedom to colloidal quantum dot optoelectronics. © 2012 American Chemical Society.

  13. Inhibition of Connexin 26/43 and Extracellular-Regulated Kinase Protein Plays a Critical Role in Melatonin Facilitated Gap Junctional Intercellular Communication in Hydrogen Peroxide-Treated HaCaT Keratinocyte Cells

    Directory of Open Access Journals (Sweden)

    Hyo-Jung Lee

    2012-01-01

    Full Text Available Though melatonin was known to regulate gap junctional intercellular communication (GJIC in chick astrocytes and mouse hepatocytes, the underlying mechanism by melatonin was not elucidated in hydrogen peroxide- (H2O2- treated HaCaT keratinocyte cells until now. In the current study, though melatonin at 2 mM and hydrogen peroxide (H2O2 at 300 μM showed weak cytotoxicity in HaCaT keratinocyte cells, melatonin significantly suppressed the formation of reactive oxygen species (ROS in H2O2-treated HaCaT cells compared to untreated controls. Also, the scrape-loading dye-transfer assay revealed that melatonin enhances the intercellular communication by introducing Lucifer Yellow into H2O2-treated cells. Furthermore, melatonin significantly enhanced the expression of connexin 26 (Cx26 and connexin 43 (Cx43 at mRNA and protein levels, but not that of connexin 30 (Cx30 in H2O2-treated HaCaT cells. Of note, melatonin attenuated the phosphorylation of extracellular signal-regulated protein kinases (ERKs more than p38 MAPK or JNK in H2O2-treated HaCaT cells. Conversely, ERK inhibitor PD98059 promoted the intercellular communication in H2O2-treated HaCaT cells. Furthermore, combined treatment of melatonin (200 μM and vitamin C (10 μg/mL significantly reduced ROS production in H2O2-treated HaCaT cells. Overall, these findings support the scientific evidences that melatonin facilitates gap junctional intercellular communication in H2O2-treated HaCaT keratinocyte cells via inhibition of connexin 26/43 and ERK as a potent chemopreventive agent.

  14. Gap junctions-guards of excitability

    DEFF Research Database (Denmark)

    Stroemlund, Line Waring; Jensen, Christa Funch; Qvortrup, Klaus

    2015-01-01

    Cardiomyocytes are connected by mechanical and electrical junctions located at the intercalated discs (IDs). Although these structures have long been known, it is becoming increasingly clear that their components interact. This review describes the involvement of the ID in electrical disturbances...... of the heart and focuses on the role of the gap junctional protein connexin 43 (Cx43). Current evidence shows that Cx43 plays a crucial role in organizing microtubules at the intercalated disc and thereby regulating the trafficking of the cardiac sodium channel NaV1.5 to the membrane....

  15. Feasibility Studies of the Two Filters Method in TJ-II for Electron Temperature Measurements in High Density Plasmas

    International Nuclear Information System (INIS)

    Baiao, D.; Medina, F.; Ochando, M.; Varandas, C.

    2009-01-01

    The TJ-II plasma soft X-ray emission was studied in order to establish an adequate setup for an electron temperature diagnostic suitable for high density, with spatial and temporal resolutions, based on the two-filters method. The preliminary experimental results reported were obtained with two diagnostics (an X-ray PHA based on a Ge detector and a tomography system) already installed in TJ-II stellarator. These results lead to the conclusion that the two-filters method was a suitable option for an electron temperature diagnostic for high-density plasmas in TJ-II. We present the design and fi rst results obtained with a prototype for the measurement of electron temperature in TJ-II plasmas heated with energetic neutral beams. This system consists in two AXUV20A detectors which measure the soft X-ray plasma emissivity trough beryllium filters of different thickness. From the two-filters technique it is possible to estimate the electron temperature. The analyses carried out allowed concluding which filter thicknesses are most suited for TJ-II plasmas, and enhanced the need of a computer code to simulate signals and plasma compositions. (Author) 7 refs.

  16. Feasibility Studies of the Two Filters Method in TJ-II for Electron Temperature Measurements in High Density Plasmas

    Energy Technology Data Exchange (ETDEWEB)

    Baiao, D.; Medina, F.; Ochando, M.; Varandas, C.

    2009-07-01

    The TJ-II plasma soft X-ray emission was studied in order to establish an adequate setup for an electron temperature diagnostic suitable for high density, with spatial and temporal resolutions, based on the two-filters method. The preliminary experimental results reported were obtained with two diagnostics (an X-ray PHA based on a Ge detector and a tomography system) already installed in TJ-II stellarator. These results lead to the conclusion that the two-filters method was a suitable option for an electron temperature diagnostic for high-density plasmas in TJ-II. We present the design and fi rst results obtained with a prototype for the measurement of electron temperature in TJ-II plasmas heated with energetic neutral beams. This system consists in two AXUV20A detectors which measure the soft X-ray plasma emissivity trough beryllium filters of different thickness. From the two-filters technique it is possible to estimate the electron temperature. The analyses carried out allowed concluding which filter thicknesses are most suited for TJ-II plasmas, and enhanced the need of a computer code to simulate signals and plasma compositions. (Author) 7 refs.

  17. The TJ-II Relational Database Access Library: A User's Guide; Libreria de Acceso a la Base de Datos Relacional de TJ-II: Guia del Usuario

    Energy Technology Data Exchange (ETDEWEB)

    Sanchez, E.; Portas, A. B.; Vega, J.

    2003-07-01

    A relational database has been developed to store data representing physical values from TJ-II discharges. This new database complements the existing TJ-EI raw data database. This database resides in a host computer running Windows 2000 Server operating system and it is managed by SQL Server. A function library has been developed that permits remote access to these data from user programs running in computers connected to TJ-II local area networks via remote procedure cali. In this document a general description of the database and its organization are provided. Also given are a detailed description of the functions included in the library and examples of how to use these functions in computer programs written in the FORTRAN and C languages. (Author) 8 refs.

  18. Review of confinement and transport studies in the TJ-II flexible heliac

    International Nuclear Information System (INIS)

    Alejaldre, C.; Alonso, J.; Almoguera, L.

    2001-01-01

    TJ-II is a four period, low magnetic shear stellarator (R=1.5m, a 0 ≤1.2T) which was designed to have a high degree of magnetic configuration flexibility. In the last experimental campaign, coupling of the full ECH power (P ECRH ≤600kW) to the plasma has been possible using two ECRH transmission lines which have different power densities. Both helium and hydrogen fuelled plasmas have been investigated. This paper reviews the latest physics results in particle control, configuration effects, and transport and fluctuation studies. (author)

  19. Numerical study of the t-J model: Exact ground state and flux phases

    International Nuclear Information System (INIS)

    Hasegawa, Y.; Poilblanc, D.

    1990-01-01

    Strongly correlated 2D electrons described by the t-J model are investigated numerically. Exact ground state for one and two holes in a finite cluster with periodic boundary conditions are obtained by using the Lanczos algorithm. The effects of Coulomb repulsion of the holes on the nearest neighbor sites are taken into account. Commensurate flux phases are investigated for the same size of clusters. They are shown to be a good approximation for the ground state specially in the intermediate value of J/t. (author). 21 refs, 3 figs

  20. Current ripple in the coils of the TJ-II Spanish stellarator

    Energy Technology Data Exchange (ETDEWEB)

    Perez, A.; Acero, J.; Alberdi, B.; Del Rio, J.M. [JEMA SA, Lasarte-Oria (Spain); Almoguera, L.; Blaumoser, M.; Kirpitchev, I.; Mendez, P. [Asociacion EURATOM-CIEMAT para Fusion, Madrid (Spain)

    1995-12-31

    High precision coil current control, stability and ripple content are very important aspects for a stellarator design. The TJ-II coils will be supplied by network commutated current converters and therefore the coil currents will contain harmonics which have to be kept to a very low level. An analytical investigation as well as numerous simulations with EMTP, SABER{reg_sign} and other softwares, have been done in order to predict the harmonic currents and to verify the completion with the specified maximum levels. The calculations and the results are presented.

  1. Slave-particle quantization and sum rules in the t-J model

    International Nuclear Information System (INIS)

    Le Guillou, J.C.; Ragoucy, E.

    1994-12-01

    In the framework of constrained systems, the classical Hamiltonian formulation of slave-particle models and their correct quantization are given. The electron-momentum distribution function in the t-J and Hubbard models is then studied in the framework of slave-particle approaches and within the decoupling scheme. It is shown that criticisms which have been addressed in this context coming from a violation of the sum rule for the physical electron are not valid. Due to the correct quantization rules for the slave-particles, the sum rule for the physical electron is indeed obeyed, both exactly and within the decoupling scheme. (author). 15 refs

  2. Einsatzmöglichkeiten des Hüttlin Kugelcoaters HKC 05-TJ unter Einbeziehung von Simulationen

    OpenAIRE

    Wöstheinrich, Karin

    2000-01-01

    Ein Wirbelschichtgerät für den Labormassstab (Hüttlin Kugelcoater HKC 05-TJ) wird instrumentiert und mit Hilfe von Lactosegranulaten, die anschliessend tablettiert werden, validiert. Ein computergestütztes Simulationsprogramm wird entwickelt, mit dem Temperaturverläufe im Gerät während des Besprühens der Modellsubstanz Glaskugeln mit Wasser sowie das Schichtenwachstum der Glaskugeln nach Besprühen mit Lactoselösung berchnet werden. Vergleiche zwischen Modell und Experiment ergeben eine gute Ü...

  3. Fabrication of the vacuum vessel of the Spanish stellarator TJ-II

    International Nuclear Information System (INIS)

    Botija, J.; Blaumoser, M.; Cal, E. de la; Garcia, A.; Tabares, F.; Molleta, L.; Rigadello, D.; Dal Maso, S.; Bevilacqua, G.

    1995-01-01

    TJ-II is a medium size stellarator under construction in Madrid, Spain. Its major plasma radius is 1.5 m and its minor plasma dimensions are 0.2m by 0.4m. The toroidal magnetic field on the axis is 1T. The bean shaped helical plasma is contained in a stainless steel vacuum vessel with a total of 96 ports, including 8 manholes to have access to its interior. The vacuum vessel will be baked at 150 C. Its complicated geometry along with the high tolerance requirements make this component a difficult manufacturing challenge. (orig.)

  4. Current ripple in the coils of the TJ-II Spanish stellarator

    International Nuclear Information System (INIS)

    Perez, A.; Acero, J.; Alberdi, B.; Del Rio, J.M.; Almoguera, L.; Blaumoser, M.; Kirpitchev, I.; Mendez, P.

    1995-01-01

    High precision coil current control, stability and ripple content are very important aspects for a stellarator design. The TJ-II coils will be supplied by network commutated current converters and therefore the coil currents will contain harmonics which have to be kept to a very low level. An analytical investigation as well as numerous simulations with EMTP, SABER reg-sign and other softwares, have been done in order to predict the harmonic currents and to verify the completion with the specified maximum levels. The calculations and the results are presented

  5. First Toroidal Rotation Measurements of Protons and Impurities in the TJ-II Stellarator

    International Nuclear Information System (INIS)

    Rapisarda, D.; Zurro, B.; Baciero, A.

    2006-01-01

    First absolute toroidal rotation measurements in the TJ-II stellarator, by using passive emission spectroscopy, are presented. The wavelength calibration is performed by using a spectral system which combines the spectra coming from the plasma and from a lamp in real time. Measurements have been made both for protons and some impurity ions (C4+, He+), in discharges created by electron cyclotron resonance heating, and in discharges with neutral beam injection heating. In addition, a description of the systems as well as the calibration procedures an data analysis is addressed. (Author) 10 refs

  6. High voltage power supplies for the neutral beam injectors of the stellarator TJ-II

    International Nuclear Information System (INIS)

    Alonso, J.; Liniers, M.; Martinez Laso, L.; Jauregi, E.; Lucia, C.; Valcarcel, F.

    2001-01-01

    Neutral beam injection will be available for the second experimental phase of TJ-II. Two injectors, set in co-counter configuration, will inject into the plasma two 40 keV H 0 beams, each of up to 1 MW. The two high voltage power supplies to feed the acceleration grids of the injectors, described in this paper, are of the transformer-rectifier type, taking their primary energy from a pulsed flywheel generator, and are coupled to the acceleration grids through a switching device. This environment effectively sets the main operation limits and protection requirements of the power supplies

  7. Exit points, on plasma, of lost fast ions during NBI in TJ-II; Puntos de salida en el plasma de los iones rapidos durante NBI en el TJ-II

    Energy Technology Data Exchange (ETDEWEB)

    Guasp, J.

    1995-07-01

    The distribution of the exit points, on plasma border, for the lost fast ions during tangential balanced NBI in TJ-II helical axis Stellarator is theoretically analysed, as well for direct as for delayed losses. The link between, the position of those exit points and the corresponding at birth, orbits and drifts is analysed also. It is shown that such relation is rather independent of beam energy and plasma density and is mainly related to the magnetic configuration characteristics. This study is a needed intermediate step to the analysis of impacts of those ions on the vacuum vessel of TJ-II. (Author) 2 refs.

  8. Plasma density determination by microwave interferometry .- The 2 mm interferometer of the TJ-1 Tokamak; Determinacion de la densidad de un plasma por interferometria de microondas. El interferometro de 2 mm del Tokamak TJ-1

    Energy Technology Data Exchange (ETDEWEB)

    Martin, R; Manero, F

    1984-07-01

    In this paper a description is given of the microwave interferometer used for measuring the plasma electronic density in the TJ-1 Tokamak of Fusion Division of JEN. The principles of the electronic density measurement are discussed in detail, as well as those concerning the determination of density pro files from experimental data. A description of the interferometer used in the TJ-1 Tokamak is given, together with a detailed analysis of the circuits which constitute the measuring chain. The working principles of the klystron reflex and hybrid rings are also presented. (Author) 23 refs.

  9. Magnetic Field Considerations for the Design and Location of a Diagnostic Neutral Beam Injector for the TJ-II Stellarator

    International Nuclear Information System (INIS)

    McCarthy, K. J.; Lopez Fraguas, A.; Balbin, R.

    2004-01-01

    A diagnostic neutral beam injection system is being developed for the TJ-II stellarator. The principal goal is to increase the signal-to-noise ratio and to provide spatial resolution along the plasma minor radius in Charge Exchange Recombination Spectroscopy and Neutral Particle Analysis diagnostics, while also opening up new opportunities for physics studies. After summarizing the compact diagnostic neutral beam injector system selected as well as the TJ-II vacuum vessel and coil geometry, we address the sensitivity of TJ-II magnetic configurations to the ferromagnetic materials that shield the ion source and neutralizer tubing of the neutral beam injection system using a popular approach in which the field is approximated via magnetic dipole moments, finally, the scientific and design trade-offs made to minimize the impact are discussed. (Author) 24 refs

  10. Transient behaviour in the plasma core of TJ-II stellarator and its relation with rational surfaces

    International Nuclear Information System (INIS)

    Estrada, T.; Luna, E. de la; Ascasibar, E; Jimenez, J.A.; Castejon, F.; Garcia-Cortes, I.; Lopez-Fraguas, A.; Sanchez, J.; Tribaldos, V.

    2002-01-01

    A transient behaviour is observed in the plasma core of TJ-II stellarator with fast drops in the electron temperature. Changes in the line-averaged density are observed synchronized with temperature drops. This phenomenon appears in plasmas created and heated using 300 kW of electron cyclotron heating with high power density. The transient behaviour resembles both, the electric pulsation discovered in CHS and the 'electron root' feature reported by the W7-AS team. The flexibility and low magnetic shear of TJ-II have permitted the identification of the plasma current as the control parameter for the appearance of this phenomenon. The results obtained during the magnetic configuration scans carried out in TJ-II points to the hypothesis that the transient behaviour is connected with the presence of a rational surface close to the plasma centre. Equilibrium calculations performed with the VMEC code reinforce this hypothesis. (author)

  11. Four-junction superconducting circuit

    Science.gov (United States)

    Qiu, Yueyin; Xiong, Wei; He, Xiao-Ling; Li, Tie-Fu; You, J. Q.

    2016-01-01

    We develop a theory for the quantum circuit consisting of a superconducting loop interrupted by four Josephson junctions and pierced by a magnetic flux (either static or time-dependent). In addition to the similarity with the typical three-junction flux qubit in the double-well regime, we demonstrate the difference of the four-junction circuit from its three-junction analogue, including its advantages over the latter. Moreover, the four-junction circuit in the single-well regime is also investigated. Our theory provides a tool to explore the physical properties of this four-junction superconducting circuit. PMID:27356619

  12. Tunable Nitride Josephson Junctions.

    Energy Technology Data Exchange (ETDEWEB)

    Missert, Nancy A. [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); Henry, Michael David [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); Lewis, Rupert M. [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); Howell, Stephen W. [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); Wolfley, Steven L. [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); Brunke, Lyle Brent [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); Wolak, Matthaeus [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)

    2017-12-01

    We have developed an ambient temperature, SiO2/Si wafer - scale process for Josephson junctions based on Nb electrodes and Ta x N barriers with tunable electronic properties. The films are fabricated by magnetron sputtering. The electronic properties of the TaxN barriers are controlled by adjusting the nitrogen flow during sputtering. This technology offers a scalable alternative to the more traditional junctions based on AlOx barriers for low - power, high - performance computing.

  13. The Normal-incidence Vacuum-ultraviolet Spectrometer for the TJ-II and First Experimental Results

    International Nuclear Information System (INIS)

    McCarthy, K. J.; Zurro, B.; Baciero, A.

    2002-01-01

    A normal-incidence spectrometer, operating in the extreme-ultraviolet and ultraviolet wavelength regions, has been commissioned for the TJ-II stellarator. The instrument has been custom built by McPherson, Chelmsford, MA, and has several unique features and accessories that are described here. The instrument and CCD detector has been tested and calibrated, and its performance evaluated, using spectral lines from glow discharges and a RF excited flow lamp. Finally, the first spectra collected with the instrument of TJ-II plasmas are presented and a preliminary estimation of an oxygen ion temperature is made. (Author) 23 refs

  14. The Normal-incidence Vacuum-ultraviolet Spectrometer for the TJ-II and First Experimental Results

    Energy Technology Data Exchange (ETDEWEB)

    McCarthy, K.J.; Zurro, B.; Baciero, A.

    2002-07-01

    A normal-incidence spectrometer, operating in the extreme-ultraviolet and ultraviolet wavelength regions, has been commissioned for the TJ-II stellarator. The instrument has been custom built by McPherson, Chelmsford, MA, and has several unique features and accessories that are described here. The instrument and CCD detector has been tested and calibrated, and its performance evaluated, using spectral lines from glow discharges and a RF excited flow lamp. Finally, the first spectra collected with the instrument of TJ-II plasmas are presented and a preliminary estimation of an oxygen ion temperature is made. (Author) 23 refs.

  15. Self consistent solution of the tJ model in the overdoped regime

    Science.gov (United States)

    Shastry, B. Sriram; Hansen, Daniel

    2013-03-01

    Detailed results from a recent microscopic theory of extremely correlated Fermi liquids, applied to the t-J model in two dimensions, are presented. The theory is to second order in a parameter λ, and is valid in the overdoped regime of the tJ model. The solution reported here is from Ref, where relevant equations given in Ref are self consistently solved for the square lattice. Thermodynamic variables and the resistivity are displayed at various densities and T for two sets of band parameters. The momentum distribution function and the renormalized electronic dispersion, its width and asymmetry are reported along principal directions of the zone. The optical conductivity is calculated. The electronic spectral function A (k , ω) probed in ARPES, is detailed with different elastic scattering parameters to account for the distinction between LASER and synchrotron ARPES. A high (binding) energy waterfall feature, sensitively dependent on the band hopping parameter t' is noted. This work was supported by DOE under Grant No. FG02-06ER46319.

  16. Commissioning of the 28 GHz ECRH power transmission line for the TJ-II stellarator

    Energy Technology Data Exchange (ETDEWEB)

    Martínez-Fernández, J., E-mail: jose.martinez@ciemat.es [Laboratorio Nacional de Fusión (LNF), Centro de Investigaciones Tecnológicas, Medioambientales y Tecnológicas (CIEMAT), Av/Complutense 40, 28040 Madrid (Spain); Cappa, Á. [Laboratorio Nacional de Fusión (LNF), Centro de Investigaciones Tecnológicas, Medioambientales y Tecnológicas (CIEMAT), Av/Complutense 40, 28040 Madrid (Spain); Chirkov, A. [Institute of Applied Physics, Russian Academy of Sciences, Nizhny Novgorod (Russian Federation); Ros, A.; Tolkachev, A.; Catalán, G.; Soleto, A.; Redondo, M. [Laboratorio Nacional de Fusión (LNF), Centro de Investigaciones Tecnológicas, Medioambientales y Tecnológicas (CIEMAT), Av/Complutense 40, 28040 Madrid (Spain); Doane, J.L.; Anderson, J.P. [General Atomics, P.O. Box 85608, San Diego, CA 92186-5608 (United States)

    2015-10-15

    Highlights: • The 28 GHz power transmission line of the TJ-II stellarator is described. • Mismatch and alignment problems are covered, presenting infrared measurements. • Beam distortion in the matching optics unit led to unwanted modes in the waveguide. • After a redesign distortion was eliminated and coupling maximized. • Final measurements suggest finer alignment must be performed. - Abstract: The commissioning of the 28 GHz power transmission line of the TJ-II stellarator, designed for the excitation of electron Bernstein waves (EBW) through the O-X-B mode conversion process, is presented in this paper. Based upon a comprehensive set of thermal measurements, its purpose is to go into details about the several problems that arouse during the whole process, namely higher order modes excitation because of the wider beam size and alignment mismatches at the waveguide mouth. All these drawbacks may have prevented the correct O-X mode conversion, thus providing a reasonable explanation for the unsuccessful EBW heating experiments.

  17. Investigation of three-dimensional turbulent structures in the torsatron TJ-K

    International Nuclear Information System (INIS)

    Mahdizadeh, N.

    2007-01-01

    In this work, for the first time, the three-dimensional nature of drift waves has been verified experimentally inside the confinement region of the toroidal plasma in TJ-K. The perpendicular dynamics of turbulence has been studied with the focus on the poloidal wavenumber spectra and the scaling of the turbulent structure with the drift scale. To this end, a 64 tip Langmuir probe array has been used, which is poloidally positioned on a flux surface. For the first time, the parallel dynamics of turbulence has been investigated in the core of a toroidally confined plasma. In contrast to previous experiments, multi-probe measurements were carried out to get simultaneous information on the shape and the propagation direction of the turbulent structures. The results for the parallel wave number and the parallel propagation velocity have been compared with results from the simulation code GEM3. It is demonstrated that the propagation in the direction parallel to the magnetic field is affected by Alfven dynamics. Together, these results strongly confirm previous investigations, which have demonstrated the importance of drift-wave turbulence in TJ-K and therefore also in fusion edge plasma. (orig.)

  18. Impurity Dynamics under Neutral Beam Injection at TJ-II (simulation)

    International Nuclear Information System (INIS)

    Guasp, J.; Fuentes, C.; Liniers, M.

    2001-01-01

    In this study the simulations of plasma transport under NBI for TJ-II, previously performed, are extended. Since than a considerable number of important modifications have been introduced in the model: change of magnetic configuration, use of experimental initial profiles, expansion of the Data base from NBI calculations and, mainly, a detailed handling of impurities with inclusion of sputtering effects. Moreover there is now a particular emphasis on the analysis of the conditions for discharge collapse and on the possible effects of single beam injection. This analysis of impurity behaviour with sputtering shows that in the expected usual cases there is no radioactive collapse and that if the recycling coefficients remain lower the unity it is always possible to find a strategy for external gas puffing leading to a stationary state, with densities below the limit and efficient NBI absorption (>50%). The radioactive collapse can appear either at high densities (central value higher than 1.4x10''20 m''3), excessive influx of impurities (i. e. with sputtering rates higher than twice the expected values) o for insufficient injected beam power (less than 45 kW). The present study analyses only the 100 4 4 6 4 configuration of TJ-II, but future works will start a systematic scan of configuration using this same model. (Author) 12 Refs

  19. El "añadido" castellano del TJ 1 o las mal llamadas "prosiflcaciones"

    Directory of Open Access Journals (Sweden)

    Montoya Martínez, Jesús

    2002-06-01

    Full Text Available The author suggests to give the name of "addition" to the first twenty-five Cantigas de Santa María, written in prose, of the Ms TJ l. The author thinks that these additions do not come neither from Alfonso X nor from his surrouindings. They should have been added when the cultural reader was different and did not know the language of Galice. What proves this is the fact that the person who wrote this "addition" obtained information from another sources in some miracles (numbers 2 and 3 and those of praise are a theological gloss.

    El autor propone que se llame "añadido" a las hasta ahora denominadas prosificaciones castellans de las primeras veinticinco cantigas de Santa María del Ms TJ l. Él entiende que no se deben a Alfonso X, ni a su entorno. Que debieren ser añadidas en un tiempo en que el lector cultural era distinto y desconocía el gallego. Esto lo demuestra en cuanto el responsable del tal "añadido" acude a fuentes distintas en algunos milagros (números 2 y 3 y las de loor son una glosa teológica.

  20. Performance of Surfactant Methyl Ester Sulphonate solution for Oil Well Stimulation in reservoir sandstone TJ Field

    Science.gov (United States)

    Eris, F. R.; Hambali, E.; Suryani, A.; Permadi, P.

    2017-05-01

    Asphaltene, paraffin, wax and sludge deposition, emulsion and water blocking are kinds ofprocess that results in a reduction of the fluid flow from the reservoir into formation which causes a decrease of oil wells productivity. Oil well Stimulation can be used as an alternative to solve oil well problems. Oil well stimulation technique requires applying of surfactant. Sodium Methyl Ester Sulphonate (SMES) of palm oil is an anionic surfactant derived from renewable natural resource that environmental friendly is one of potential surfactant types that can be used in oil well stimulation. This study was aimed at formulation SMES as well stimulation agent that can identify phase transitions to phase behavior in a brine-surfactant-oil system and altered the wettability of rock sandstone and limestone. Performance of SMES solution tested by thermal stability test, phase behavioral examination and rocks wettability test. The results showed that SMES solution (SMES 5% + xylene 5% in the diesel with addition of 1% NaCl at TJformation water and SMES 5% + xylene 5% in methyl ester with the addition of NaCl 1% in the TJ formation water) are surfactant that can maintain thermal stability, can mostly altered the wettability toward water-wet in sandstone reservoir, TJ Field.

  1. Magnetic configuration and transport interplay in TJ-II flexible heliac

    International Nuclear Information System (INIS)

    Alejaldre, C.; Alonso, J.; Almoguera, L.

    2003-01-01

    This paper presents an overview of experimental results and progress in the investigation of the role of the magnetic configuration on stability and transport in the TJ-II stellarator. Significant improvement in the characterization of confinement and stability properties of TJ-II stellarator plasmas has been recently achieved. Global confinement studies have shown a positive dependence of energy confinement on rotational transform, reinforcing the dependence found with the ISS95 database. Spontaneous transitions in particle and energy confinement have been observed which resemble some characteristics of previously reported H-mode regimes in other stellarator devices. Magnetic configuration scan experiments have shown the interplay between magnetic topology (e.g. rationals), transport and electric fields. Cold pulse as well as the transport events provoked by decreasing magnetic well generates non-diffusive propagation. First measurements of radial electric fields and plasma potential show values that are comparable with those expected from neoclassical calculations. Active biasing experiments have shown an impact both in edge and global plasma parameters. In low magnetic well configurations sheared edge poloidal and parallel flows are linked near marginal stability. (author)

  2. Effects of electrode polarization and particle deposition profile on TJ-I plasma confinement

    International Nuclear Information System (INIS)

    Zurro, B.; Tabares, F.; Pardo, C.; Tafalla, D.; Cal, E. de la; Garcia-Castaner, B.; Pedrosa, M.A.; Sanchez, J.; Rodriguez-Yunta, A.

    1991-01-01

    The role of self-created radial electric field on particle confinement in TJ-I plasmas was addressed using plasma rotation data in conjunction with particle confinement times measured by laser ablation. In this paper following the pioneer work of Taylor, we have started to study the influence of a polarized electrode inserted into the plasma on particle confinement and plasma rotation in this ohmically heated tokamak. To have a supportive frame of reference, the confinement time of background particles and their transport into plasma without electrode, has been studied by measuring with space-time resolution the H α emission on varying plasma conditions. These experiments have been carried out in ohmically heated discharges of the TJ-I tokamak (R 0 =30 cm, a=10 cm) which was operated with plasma currents between 20 and 45 kA and a toroidal field ranging from 0.8 to 1.5 T. In this paper, firstly the experimental plasma and specific diagnostics are described, secondly, the parametric dependence of the particle confinement time and radial transport of background plasma is presented and finally, the influence of polarizing an inserted electrode on a particular discharge is given and discussed in the context of other polarization experiments. (author) 7 refs., 4 figs

  3. Transmission of the Neutral Beam Heating Beams at TJ-II

    International Nuclear Information System (INIS)

    Fuentes Lopez, C.

    2007-01-01

    Neutral beam injection heating has been development for the TJ-II stellarator. The beam has a port-through power between 700-1500 kW and injection energy 40 keV. The sensibility of the injection system to the changes of several parameters is analysed. Beam transmission is limited by losses processes since beam is born into the ions source until is coming into the fusion machine. For the beam transmission optimization several beam diagnostics have been developed. A carbon fiber composite (CFC) target calorimeter has been installed at TJ-II to study in situ the power density distribution of the neutral beams. The thermographic print of the beam can be recorded and analysed in a reliable way due to the highly anisotropic thermal conductivity of the target material. With the combined thermographic and calorimetric measurements it has been possible to determine the power density distribution of the beam. It has been found that a large beam halo is present, which can be explained by the extreme misalignment of the grids. This kind of halo has a deleterious effect on beam transport and must be minimized in order to improve the plasma heating capability of the beams. (Author) 155 refs

  4. Investigation of three-dimensional turbulent structures in the torsatron TJ-K

    Energy Technology Data Exchange (ETDEWEB)

    Mahdizadeh, N.

    2007-02-14

    In this work, for the first time, the three-dimensional nature of drift waves has been verified experimentally inside the confinement region of the toroidal plasma in TJ-K. The perpendicular dynamics of turbulence has been studied with the focus on the poloidal wavenumber spectra and the scaling of the turbulent structure with the drift scale. To this end, a 64 tip Langmuir probe array has been used, which is poloidally positioned on a flux surface. For the first time, the parallel dynamics of turbulence has been investigated in the core of a toroidally confined plasma. In contrast to previous experiments, multi-probe measurements were carried out to get simultaneous information on the shape and the propagation direction of the turbulent structures. The results for the parallel wave number and the parallel propagation velocity have been compared with results from the simulation code GEM3. It is demonstrated that the propagation in the direction parallel to the magnetic field is affected by Alfven dynamics. Together, these results strongly confirm previous investigations, which have demonstrated the importance of drift-wave turbulence in TJ-K and therefore also in fusion edge plasma. (orig.)

  5. Molecular anatomy of interendothelial junctions in human blood-brain barrier microvessels.

    Directory of Open Access Journals (Sweden)

    Andrzej W Vorbrodt

    2004-07-01

    Full Text Available Immunogold cytochemical procedure was used to study the localization at the ultrastructural level of interendothelial junction-associated protein molecules in the human brain blood microvessels, representing the anatomic site of the blood-brain barrier (BBB. Ultrathin sections of Lowicryl K4M-embedded biopsy specimens of human cerebral cortex obtained during surgical procedures were exposed to specific antibodies, followed by colloidal gold-labeled secondary antibodies. All tight junction-specific integral membrane (transmembrane proteins--occludin, junctional adhesion molecule (JAM-1, and claudin-5--as well as peripheral zonula occludens protein (ZO-1 were highly expressed. Immunoreactivity of the adherens junction-specific transmembrane protein VE-cadherin was of almost similar intensity. Immunolabeling of the adherens junction-associated peripheral proteins--alpha-catenin, beta-catenin, and p120 catenin--although positive, was evidently less intense. The expression of gamma-catenin (plakoglobin was considered questionable because solitary immunosignals (gold particles appeared in only a few microvascular profiles. Double labeling of some sections made possible to observe strict colocalization of the junctional molecules, such as occludin and ZO-1 or JAM-1 and VE-cadherin, in the interendothelial junctions. We found that in human brain microvessels, the interendothelial junctional complexes contain molecular components specific for both tight and adherens junctions. It is assumed that the data obtained can help us find the immunodetectable junctional molecules that can serve as sensitive markers of normal or abnormal function of the BBB.

  6. Quantum Junction Solar Cells

    KAUST Repository

    Tang, Jiang; Liu, Huan; Zhitomirsky, David; Hoogland, Sjoerd; Wang, Xihua; Furukawa, Melissa; Levina, Larissa; Sargent, Edward H.

    2012-01-01

    -performing colloidal quantum dot rectifying devices reported to date have relied on a junction between a quantum-tuned absorber and a bulk material (e.g., TiO 2); however, quantum tuning of the absorber then requires complete redesign of the bulk acceptor, compromising

  7. Zinc ions regulate opening of tight junction favouring efflux of macromolecules via the GSK3β/snail-mediated pathway.

    Science.gov (United States)

    Xiao, Ruyue; Yuan, Lan; He, Weijiang; Yang, Xiaoda

    2018-01-24

    Zinc is an essential trace element presenting in particularly high concentration in the brain. In some regions, e.g. lateral amygdala, subiculum and hippocampus, rapidly-exchangeable zinc may transiently reach even up to 600 μM. To explore the possible roles of high-concentration Zn 2+ in regulating the blood-brain barrier (BBB), we investigated the effects of Zn 2+ on the functions and structures of the tight junction (TJ) with an in vitro model of a Madin-Darby canine kidney (MDCK) cell monolayer. The experimental results indicated that high concentrations (>200 μM) of Zn 2+ can affect the TJ integrity in a polarized manner. Basolateral addition of Zn 2+ led to reversible TJ opening with pore paths of r ∼ 2 nm or more depending on Zn 2+ concentration. The efflux/influx ratios of different sized probes were found to be ∼4.6 for FD4 (M W 4000) and ∼1.8 for Eu-DTPA (M W 560), suggesting that the Zn 2+ -induced paracelluar channels favour efflux especially for macromolecules. Further mechanistic studies revealed that the elevated intracellular Zn 2+ taken from the basolateral side can increase phosphorylation of glycogen synthase kinase (GSK) 3β, primarily due to the inhibition of calcineurin (CaN), thus resulting in the elevation of the snail transcriptional repressors. Subsequently, Zn 2+ can cause the down-regulation of claudin-1, breakage of occludin and ZO-1 rings, and collapse of basolateral F-actin structures. These overall factors result in the formation of a trumpet-like paracellular channel, which allows asymmetric solute permeation. The ERK1/2 and JNK1/2 pathways may also be involved in the Zn 2+ -induced TJ opening process, while the activation of matrix metalloproteinase was not observed. Our results may suggest a potential role of zinc in regulation of BBB permeability associated with brain clearance of metabolites through the glymphatic system.

  8. NBI Calculations for the TJ-II Experimental Discharges; Ajustes de los Perfiles Radiales de Densidad y Temperatura para las Descargas con NBI del TJ-II

    Energy Technology Data Exchange (ETDEWEB)

    Guasp, J.; Fuentes, C.; Liniers, M.

    2005-07-01

    The density and electron temperature radial profiles, corresponding to the experimental TJ-II campaigns 2003-2004, with NBI, have been fitted to simple functionals in order to allow a fast approximative evaluation for any given density and injected power... The fits have been calculated, separately, for the four possibilities: ECRH and NBI Phases as well as On and Off Axis ECRH injection. The average difference between the experimental profiles for the individual discharges and the fit predictions are around 8% for the density and 10% for the temperature. The behaviour of the predicted profiles with average line density and injected power has been analysed. The central electron temperature decreases monotonically with increasing density and the ECRH phase On Axis central value is clearly higher than the Off axis one. The radial density profiles narrow with increasing density and the NBI On axis case is clearly wider than de Off one. The electron temperature profile widens slightly with increasing density and the width of the On Axix case is lesser than for the Off case in all phases. There exist Fortran subroutines, available at the three CIEMAT computers, allowing the fast approximative evaluation of all these profiles. (Author) 8 refs.

  9. Competing States in the t-J Model: Uniform d-Wave State versus Stripe State versus Stripe State

    NARCIS (Netherlands)

    Corboz, P.R.; Rice, T.M.; Troyer, M.

    2014-01-01

    Variational studies of the t-J model on the square lattice based on infinite projected-entangled pair states confirm an extremely close competition between a uniform d-wave superconducting state and different stripe states. The site-centered stripe with an in-phase d-wave order has an equal or only

  10. 热应激对奶山羊瘤胃黏膜紧密连接蛋白表达的影响%Effects of Heat Stress on Expressions of Tight Junction Proteins in Ruminal Mucosa of Dairy Goats

    Institute of Scientific and Technical Information of China (English)

    马燕芬; 杜瑞平; 高民

    2014-01-01

    本试验旨在研究热应激对奶山羊瘤胃黏膜紧密连接蛋白表达的影响。将8只体况良好,体重、年龄、胎次和产奶量相近,处于泌乳中后期的奶山羊随机分为对照组和热应激组,每组4只羊。饲养环境采取人工控温控湿。试验期45 d,在试验30和45 d时,采集瘤胃背囊和腹囊组织,分别采用免疫组化法、实时定量PCR法和蛋白质免疫印迹法检测其闭合蛋白( claudin )、闭锁小带蛋白1( ZO-1)蛋白的结构分布、基因mRNA表达量和蛋白表达量。结果表明:与对照组相比,热应激组(30 d)背囊和腹囊中claudin、ZO-1蛋白的信号强度和表达量均有所下降,而热应激组(45 d)下降更为严重。与对照组相比,热应激组(30 d)背囊和腹囊中claudin、ZO-1基因mRNA表达量均显著下降(P<0.05),而热应激组(45 d)则显著低于热应激组(30 d)(P<0.05)。结果提示,热应激对瘤胃黏膜的结构和完整性的破坏与瘤胃黏膜紧密连接蛋白表达量下降有关。%This study was conducted to study the effects of heat stress ( HS) on expressions of tight junction proteins in ruminal mucosa of dairy goats. Eight healthy dietary goats with similar body weight, age, parity and milk yield at mid to late-locating period were randomly divided into control group and heat stress ( HS ) group with 4 goats in each group. Environmental temperature and humidity during feeding period were artifi-cially controlled. The experiment lasted for 45 days. Knapsack and abdominal sac samples were collected at 30 and 45 days of experiment to determine protein structural distribution, gene mRNA expression levels and pro-tein expression levels of claudin and zonula occluden 1 ( ZO-1 ) using the methods of immunohistochemisty, real-time quantitative PCR and West-bloting, respectively. The results showed as follows: compared with the control group, expression levels and signal intensity of claudin and ZO-1 proteins in knapsack and abdominal in HS

  11. Density Dependence of Particle Transport in ECH Plasmas of the TJ-II Stellarator

    Energy Technology Data Exchange (ETDEWEB)

    Vargas, V. I.; Lopez-Bruna, D.; Guasp, J.; Herranz, J.; Estrada, T.; Medina, F.; Ochando, M.A.; Velasco, J.L.; Reynolds, J.M.; Ferreira, J.A.; Tafalla, D.; Castejon, F.; Salas, A.

    2009-05-21

    We present the experimental dependence of particle transport on average density in electron cyclotron heated (ECH) hydrogen plasmas of the TJ-II stellarator. The results are based on: (I) electron density and temperature data from Thomson Scattering and reflectometry diagnostics; (II) a transport model that reproduces the particle density profiles in steady state; and (III) Eirene, a code for neutrals transport that calculates the particle source in the plasma from the particle confinement time and the appropriate geometry of the machine/plasma. After estimating an effective particle diffusivity and the particle confinement time, a threshold density separating qualitatively and quantitatively different plasma transport regimes is found. The poor confinement times found below the threshold are coincident with the presence of ECH-induced fast electron losses and a positive radial electric field all over the plasma. (Author) 40 refs.

  12. Software package evaluation for the TJ-II Data Acquisition System

    International Nuclear Information System (INIS)

    Cremy, C.; Sanchez, E.; Portas, A.; Vega, J.

    1996-01-01

    The TJ-II Data Acquisition System (DAS) has to provide a user interface which will allow setup for sampling channels, discharge signal visualization and reduce data processing, all in run time. On the other hand, the DAS will provide a high level software capability for signal analysis, processing and data visualization either in run time or off line. A set of software packages including Builder Xcessory, X-designer, llog Builder, Toolmaster, AVS 5, AVS/Express, PV-WAVE and Iris Explorer, have been evaluated by the Data Acquisition Group of the Fusion Division. the software evaluation, resumed in this paper, has resulted in a global solution being found which meets all of the DAS requirements. (Author)

  13. Image processing methods for noise reduction in the TJ-II Thomson Scattering diagnostic

    Energy Technology Data Exchange (ETDEWEB)

    Dormido-Canto, S., E-mail: sebas@dia.uned.es [Departamento de Informatica y Automatica, UNED, Madrid 28040 (Spain); Farias, G. [Pontificia Universidad Catolica de Valparaiso, Valparaiso (Chile); Vega, J.; Pastor, I. [Asociacion EURATOM/CIEMAT para Fusion, Madrid 28040 (Spain)

    2012-12-15

    Highlights: Black-Right-Pointing-Pointer We describe an approach in order to reduce or mitigate the stray-light on the images and show the exceptional results. Black-Right-Pointing-Pointer We analyze the parameters to take account in the proposed process. Black-Right-Pointing-Pointer We report a simplified exampled in order to explain the proposed process. - Abstract: The Thomsom Scattering diagnostic of the TJ-II stellarator provides temperature and density profiles. The CCD camera acquires images corrupted with noise that, in some cases, can produce unreliable profiles. The main source of noise is the so-called stray-light. In this paper we describe an approach that allows mitigation of the effects that stray-light has on the images: extraction regions with connected-components. In addition, the robustness and effectiveness of the noise reduction technique is validated in two ways: (1) supervised classification and (2) comparison of electron temperature profiles.

  14. Control of the Superconducting Magnets current Power Supplies of the TJ-II Gyrotrons

    International Nuclear Information System (INIS)

    Ros, A.; Fernandez, A.; Tolkachev, A.; Catalan, G.

    2006-01-01

    The TJ-II ECRH heating system consists of two gyrotrons, which can deliver a maximum power of 300 kW at a frequency of 53.2 GHz. Another 28 GHz gyrotron is going to be used in the Bernstein waves heating system. In order to get the required frequency, the gyrotrons need and homogeneous magnetic field of several tesla, which is generated by a superconducting coil field by a current source. This document describes the current source control as well as the high precision ammeters control. These ammeters measure the current in the superconducting coils. The user interface and the programming of the control system are described. The communication between devices is also explained. (author) 9 Refs

  15. Experimental investigation of edge sheared flow development and configuration effects in the TJ-II stellarator

    Energy Technology Data Exchange (ETDEWEB)

    Pedrosa, M. A.; Hidalgo, C.; Alonso, A.; Calderon, E.; Orozco, R. O.; Pablos, J. L. de

    2005-07-01

    It is well known the importance of the shear as a stabilizing mechanism to control plasma fluctuations in magnetically confined plasmas [1]. It has been clearly established that Ex B shear stabilization mechanisms are an important piece for the improvement of confinement on fusion devices. In particular both edge and core transport barriers are related to a large increase in the Ex B sheared flow. As a consequence clarifying the driving mechanisms of sheared flow in fusion plasmas is a main issue. The existence of parallel and perpendicular sheared flows at the plasma edge, and the interplay between them in different plasma conditions has been studied in the TJ-II [2]. Recent experiments carried out by means of different approaches in the TJ-II stellarator have shown that the generation of spontaneous edge perpendicular sheared flow can be externally controlled by means of plasma density with good reproducibility and reliability [3, 4]. Although experimentally the plasma density has been used as an external control knob, it would be more appropriate to characterize experimental results in terms of edge plasma gradient (e.g. ion saturation current gradient) [3]. It has also been found that there exists a coupling between the onset of sheared flow development and an increase in the level of plasma edge turbulence; once sheared flow is fully developed the level of fluctuations and turbulent transport slightly decreases whereas edge gradients and plasma density increase. It has been experimentally established that the minimum plasma density (or/and minimum level of plasma turbulence) essential for the development of the shear layer depends on the plasma magnetic configuration [5, 6]. For some plasma magnetic configurations with high iota value a sheared flow-induced regime with characteristics resembling those of an improved confinement one has been found. The similarity in the structure of the velocity shear layer and in the turbulence characteristics [7] in different

  16. Modification of the Heating Position Using a Moveable Mirror in the TJ-II ECRH System

    International Nuclear Information System (INIS)

    Cappa, A.; Tribaldos, V.; Likin, K.; Fernandez, A.; Martin, R.

    1999-01-01

    During the first stages of operation, start-up and heating of plasmas in TJ-II stellarator are being produced by EC waves. These are launched by two 1/2-MW type gyrotrons at 53.2 GHz and transmitted to the plasma by two quasi-optical transmission lines located at two symmetrical stellarator positions. The last mirror of both lines, placed inside the vacuum vessel, is a moveable mirror allowing for changes in the final direction of the microwave beam and therefore in the heating position. This report is devoted to the calculations describing the movement of this mirror and its influence in the position of the reflected beam. (Author)

  17. Remote Control System of the TJ-II Microwave Transmission Lines Mirrors

    International Nuclear Information System (INIS)

    Lopez Sanchez, A.; Fernandez, A.; Cappa, A.; Gama, J. de la; Olivares, J.; Garcia, R.; Chamorro, M.

    2007-01-01

    The ECRH system of the TJ-II stellarator has two gyrotrons, which deliver a maximum power of 300 kW each at a frequency of 53.2 GHz. Another 28 GHz gyrotron will be used to heat the plasma by electron Bernstein waves (EBWH). The microwave power is transmitted from the gyrotrons to the vacuum chamber by two quasi-optical transmission lines for ECRH and a corrugated waveguide for EBWH. All transmission lines have an internal movable mirror inside the vacuum chamber to focus the beam and to be able to change the launching angle. The control of the beam polarization is very important and the lines have two corrugated mirrors, which actuate as polarizers. In this report the control system of the position of these three internal mirrors and the polarizers of the EBWH transmission line is described. (Author) 20 refs

  18. Quasi-Optical Transmission Lines for ECRH on TJ-II Stellarator

    International Nuclear Information System (INIS)

    Fernandez, A.; Likin, K.; Martin, R.; Cappa, A.; Cepero, J.R.

    1999-01-01

    Two mirror lines are used to transmit ht microwave power from the powerful microwave generators to the TJ-II plasmas. Both lines have been tested at nominal power level and they are now in operation. This paper is devoted to the final design of the transmission lines and their testing. Before starting operation at high power level measurements of the wave beam parameters at low power level were made. Two horn antennae were designed to simulate the gyrotron output. Numerical simulations of the far field radiation pattern of the antennae were made, a computer code based on Huygens diffraction theory was developed to simulate the propagation of the beam along a mirror line. A comparison of the theoretical and the experimental results is also presented here. (Author)

  19. Sensibility Studies for the Neutral Beam Injection System in TJ-II

    International Nuclear Information System (INIS)

    Fuentes, C.; Liniers, M.; Guasp, J.

    1999-01-01

    The sensibility of the Neutral Beam Injection system of TJ-II to the changes of several parameters is analysed. Transmission, absorption and power loads at the intercepting structures are evaluated. The adopted values for the ion source distance, focal length and divergence are confirmed as optimal, showing a small sensitivity to changes, except for the divergence. The operational margins for beam misalignments has been found to be small but feasible, confirming also the reference directions as optimal. Finally four possible alternatives, intended to reduce the power loads at the beam entering structures, are analysed. All of them have been discarded since lead to the appearance of new risk zones, with unacceptable load levels, and reduce the transmitted power. (Author) 13 refs

  20. Spectroscopic system for impurity measurements in the TJ-1 Tokamak of JEN

    International Nuclear Information System (INIS)

    Navas, G.; Zurro, B.

    1982-01-01

    we describe a spectroscopic system with spatial resolution capability that has been configured for plasma diagnostic in the TJ-1 Tokamak of JEN. The experimental system, based on a one meter monochromator, has been absolutely calibrated using a tungsten-halogen lamp. The calibration procedures and the absolute spectral sensitivity are presented as well as its dependence with the polarization. A simplified spectroscopic model of the radiation emitted by the intrinsic plasma impurities (C, 0, . . . ) has been developed. A one dimensional model of the temporal evolution of various ionization stages in coronal equilibrium is used to predict the electron temperature and impurity concentration. This model has been applied to experimental data from several Tokamaks. (Author) 23 refs

  1. Application of the OPTIMUS Code to the Neutral Beam Injection System of TJ-II

    International Nuclear Information System (INIS)

    Fuentes, C.; Liniers, M.; Guasp, J.

    1998-01-01

    The different losses processes affecting a neutral beam since is born into the ions source until is coming into the fusion machine, are dependent of the residual gas pressure distribution inside injector. The OPTIMUS code analyzes that losses and calculates the pressure distribution inside one injector with specific geometry. The adaptation of injector to TJ-II has not required important design changes, only the operating range of the gas flow and the pumping speed have modified. The calculations show that the required gas flows for the optimal operation of the system ned an independent pumping system for the calorimeter box with a pumping speed of 1200001/s. The system efficiency is not affected by an hypothetical beaming effect and it is found also that with a proper conditioning of the injector walls, so that the absorption coefficients do not surpass excessively the unity value, the injector operation remains optimal. (Author) 8 refs

  2. Conditioning of TJ-II Stellarator during the ECRH Plasmas Period

    International Nuclear Information System (INIS)

    Tafalla, D.; Tabares, F. L.

    2001-01-01

    The TJ-II stellarator has been conditioned by glow discharge (GD) during the first campaigns of operation, working only with ECR heating and all metal walls. The application of a He GD during the overnight period before the operation has been required in order to obtain reproducible discharges. However, the density control of the ECRH discharges was not possible because of the He implanted on the wall during GS. An short Ar GD(≤30 min) applied before the operation allows desorbes part of the implanted He. By applying this procedure (HeGD+ArGD), reproducible and density controlled plasmas have been achieved in H 2 and He. (Author) 20 refs

  3. Electron internal transport barriers and magnetic topology in the stellarator TJ-II

    Energy Technology Data Exchange (ETDEWEB)

    Estrada, T.; Lopez-Bruna, D.; Alosno, A.; Ascasibar, E.; Baciero, A.; Cappa, A.; Castejon, F.; Fernandez, A.; Herranz, J.; Hidalgo, C.; Pablos, J. L. de; Pastor, I.; Sanchez, E.; Sanchez, J.

    2005-07-01

    In most helical systems electron Internal Transport Barriers (e-ITB) are observed in Electron Cyclotron Heated (ECH) plasmas with high heating power density. In the stellarator TJ-II, e- ITBs are easily achievable by positioning a low order rational surface close to the plasma core, because this increases the density range in which the e-ITB can form. Experiments with different low order rationals show a dependence of the threshold density and barrier quality on the order of the rational (3/2, 4/2, 5/3, ...). In addition, during the formation of e-ITB quasicoherent modes are frequently observed in the plasma core region. The mode can exist before or after the e-ITB phenomenon at the radial location of the transport barrier foot but vanishes as the barrier is fully developed. (Author)

  4. Electron internal transport barrier in the core of TJ-II ECH plasmas

    Energy Technology Data Exchange (ETDEWEB)

    Estrada, T.; Hidalgo, C. [Laboratorio Nacional de Fusion por Confinamiento Magnetico. Asociacion EURATOM CIEMAT, Madrid (Spain); Dreval, N. [and others

    2003-07-01

    The influence of the magnetic topology on the formation of electron internal transport barriers (e-ITB) has been experimentally studied in the stellarator TJ-II. The formation of e-ITBs in electron cyclotron heated plasmas can be triggered by positioning a low order rational surface close to the plasma core region, while in configurations without any low order rational there are no indications of barrier formation within the available heating power. The e-ITB formation is characterized by an increase in the core electron temperature and plasma potential. Positive radial electric field increases in a factor of three in the plasma central region when the e-ITB forms. The results demonstrate that low order rational surfaces modify radial electric fields and electron heat transport. (orig.)

  5. Bosonization of the two-dimensional t-J model in the continuum limit

    International Nuclear Information System (INIS)

    Schmeltzer, D.; Bishop, A.R.

    1996-01-01

    The t-J model in two dimensions is bosonized using a set of N, coupled two-dimensional Fermi-surface patches. Ignoring tunneling between the patches, the coherent tunneling of holes and the superfluid phase are suppressed. Within this scheme the system remains in the normal phase when temperature T→0. The main feature of this construction is the absence of screening of the dissipative transversal gauge field generated by the spinons. This dissipative gauge field is responsible for the non-Fermi-liquid behavior, which is manifested in the free energy and single-particle Green function. The deviation from Fermi-liquid behavior is due to the U(1) gauge field, and at long distances a new exponent due to the holes is identified. Experimental consequences are discussed. copyright 1996 The American Physical Society

  6. Magnetic Shear and Transport in ECRH Discharges of the TJ-II under Ohmic Induction

    Energy Technology Data Exchange (ETDEWEB)

    Lopez-Bruna, D.; Castejon, F.; Romero, J. A.; Estrada, T.; Medina, F.; Ochando, M.; Lopez-Fraguas, A.; Ascasibar, E.; Herranz, J.; Sanchez, E.; Luna, E. de la; Pastor, I.

    2006-07-01

    TJ-II is ha heliac type stellarator characterised by high, but almost constant, vacuum rotational transform throughout the confining volume. In ECRH plasmas, moderate induced ohmic currents (negligible heating and modification of the magnetic field nodules) are enough to disregard the bootstrap contribution, which allows us performing a fair calculation of the evolution of the rotational transform. We use the loop voltage diagnostic to estimate the plasma electrical conductivity. Then the evolution of the rotational transform and shear is related to changes in the profiles of electron and thermal diffusivities: negative shear correlates with decreasing diffusivities in the region of steepest density gradient; transport increases toward zero shear but the achieved positive values are too small to draw conclusions. The radial sweeping of lowest order rational magnetic surfaces does not determine the observed trends in transport. (Author)43 refs.

  7. Nonplanar spiral states of the t-J model with classical spins

    International Nuclear Information System (INIS)

    Hamada, M.; Shimahara, H.; Mori, H.

    1995-01-01

    The spiral state in the two-dimensional t-J model is studied by numerical diagonalization of an effective Hamiltonian. We examine all possibilities of the spiral spin states including the nonplanar states. It is found that nonplanar spiral states occur, but the deviations from the planar spiral state in the nonplanar spiral states are small for small hole concentrations where our effective Hamiltonian is valid. The modulation of the spin configuration increases continuously from the antiferromagnetic order as the hole concentration increases, and discontinuously changes at a critical hole concentration. Then the state undergoes the first-order phase transition either to the (π,0) phase or to the ferromagnetic phase, depending on the value of J/t

  8. Los campos fantasmas en la renormalización de un modelo Lagrangiano t-J

    Directory of Open Access Journals (Sweden)

    Oscar P. Zandron

    2011-01-01

    Full Text Available En presente trabajo hemos tratado el rol de los campos fantasmas en el proceso de renormalización del formalismo Lagrangiano perturbativo para un modelo t-J. Mostramos que con la incorporación de campos fantasmas apropiados en los propagadores y vértices de la teoría, la misma puede ser renormalizada a todo orden. Esto se pone en evidencia en el estudio detallado de la renormalización del propagador ferromagnético del magnón, mostrando el ablandamiento térmico de la frecuencia. También hemos analizado el caso antiferromagnético, y estos resultados fueron confrontados con estudios previos en teorías del "spin-polarón".

  9. Magnetic Shear and Transport in ECRH Discharges of the TJ-II under Ohmic Induction

    International Nuclear Information System (INIS)

    Lopez-Bruna, D.; Castejon, F.; Romero, J. A.; Estrada, T.; Medina, F.; Ochando, M.; Lopez-Fraguas, A.; Ascasibar, E.; Herranz, J.; Sanchez, E.; Luna, E. de la; Pastor, I.

    2006-01-01

    TJ-II is ha heliac type stellarator characterised by high, but almost constant, vacuum rotational transform throughout the confining volume. In ECRH plasmas, moderate induced ohmic currents (negligible heating and modification of the magnetic field nodules) are enough to disregard the bootstrap contribution, which allows us performing a fair calculation of the evolution of the rotational transform. We use the loop voltage diagnostic to estimate the plasma electrical conductivity. Then the evolution of the rotational transform and shear is related to changes in the profiles of electron and thermal diffusivities: negative shear correlates with decreasing diffusivities in the region of steepest density gradient; transport increases toward zero shear but the achieved positive values are too small to draw conclusions. The radial sweeping of lowest order rational magnetic surfaces does not determine the observed trends in transport. (Author)43 refs

  10. Behaviour of direct and delayed fast ion losses during NBI on TJ-II

    International Nuclear Information System (INIS)

    Guasp, J.; Liniers; M.

    1995-09-01

    The dependence with density and beam energy of the different kind of fast ion losses, direct and delayed, during tangential balanced NBI injection in TJ-II helical axis stellerator has been analysed. Direct losses increase with energy and a strong difference between the two injection directions appears, are produced by passing particles that loss confinement in a dew mu,sec and the influence of birth profiles produces an increase with density. Delayed losses are very well separated in time from direct ones, are produced by particles experimenting pitch angle scattering an,d, most o them, correspond to trapped particles. Are much less important than the direct ones (about 1/3), decrease slowly with energy and, with CX, increase with density (an effect of initial profile). The absorption is rather independent of energy with low values at low density in reason of high shine trough and CX losses, but reovers quickly with the density increase

  11. Impacts of lost fast ions on the TJ-II Vacuum Vessel during NBI

    International Nuclear Information System (INIS)

    Guasp, J.

    1995-01-01

    The possible deposition patterns, on the Vacuum Vessel, of lost fast ions during the balanced tangential NBI in TJ-II helical axis Stellarator are analysed theoretically, establishing the relation between those impact points, the plasma exit and birth positions and the magnetic configuration characteristics. It is shown that direct losses are the most important, mainly those produced by the beam injected with the same direction that the magnetic field, increasing with beam energy and plasma density but with impacts remaining fixed on well defined zones, a periodically distributed along the Hard Core cover plates, producing high loads at high densities. The remaining losses, except for the shine through ones that predominate at low density, are periodically distributed, with smooth maxima and produce very low loads. No overlapping between the different kind of losses or beams is observed. (Author) 6 refs

  12. Behaviour of direct and delayed fast ion losses during NBI on TJ-II

    International Nuclear Information System (INIS)

    Guasp, J.; Liniers, M.

    1995-01-01

    The dependence with density and beam energy of the different kind of fast ion losses, direct and delayed, during tangential balanced NBI injection in TJ-II helical axis stellarator has been analysed. Direct losses increase with energy and a strong difference between the two injection directions appears, are produced by passing particles that loss confinement in a few μsec and the influence of birth profiles produces an increase with density. Delayed losses are very well separated in time from direct ones, are produced by particles experimenting pitch angle scattering and, most o them, correspond to trapped particles. Are much less important than the direct ones (about 1/3), decrease slowly with energy and, with C X, increase with density (an effect of initial profile). The absorption is rather independent of energy with low values at low density in reason of high shine through and C X losses, but recovers quickly with the density increase. (Author) 4 refs

  13. Impacts of the CX neutrals on the Vacuum Vessel of TJ-II during NBI

    International Nuclear Information System (INIS)

    Guasp, J.

    1995-09-01

    A numerical analysis of the impact patterns on the Vacuum Vessel produced by CX neutrals during the tangential balanced NBI in TJ-II Helical Axis Stellerator has been done. The results show periodical distribution with smooth maxima and mild loads, concentrated prefentlyon the HC plates. A certain preference of these neutral to emerge downwards from the plasma appears, as consequence of a similar trend for the trapped particles. The differences between the impacts produced by the beam paralel to the magnetic field and the opposite one are small, once more as a consequence of the loss of memory of trapped particles to initial direction. The dependence of loads with plasma density and beam energy follows the trend of CX losses, decreasing strongly with increasing density and decreasing, more smoothly, with energy

  14. Impacts of the Shine Through neutrals on the Vacuum Vessel of TJ-II during NBI

    International Nuclear Information System (INIS)

    Guasp, J.; Liniers, M.

    1995-01-01

    A numerical analysis of the impact patterns on the Vacuum Vessel produced by Shine Through neutrals during the tangential balanced NBI in TJ-II Helical Axis Stellarator has been done. The results show two main concentrations. The first one the circular part of the Hard Core, near the zone of closest approach of the beam. The second one, the most important, around the beam exit, on the border between the plate of the HC cover and the sector wall. As expected, the Shine Through loads decrease strongly with plasma density, predominating at low density at NBI start, decreasing quickly when density increases and increasing slightly with the beam energy. No overlapping with lost fast ions impacts is observed, that, in addition, show an opposite behaviour with density. (Author) 3 refs

  15. Impacts of the Shine Through neutrals on the Vacuum Vessel of TJ-II during NBI

    International Nuclear Information System (INIS)

    Guasp, J.; Liniers, M.

    1995-09-01

    A numerical analysis of the impact patterns on the Vacuum Vessel produced by Shine through neutrals during the tangential balanced NBI in TJ-II Helical Axis Stellarator has been done. The results show two main concentrations. The first one the circular part of the Hard Core, near the zone of closest approach of the beam. The second one, the most important, around the beam exit, on the border between the plate of the HC cover and the sector wall. As expected, the Shine through loads decrease strongly with plasma density, predominating at low density at NBI start, decreasing quickly when density increases and increasing slightly with the beam energy. No overlapping with lost fast ions impacts is observed, that, in addition, show an opposite behaviour with density

  16. Impacts of the CX neutrals on the Vacuum Vessel of TJ-II during NBI

    International Nuclear Information System (INIS)

    Guasp, J.

    1995-01-01

    A numerical analysis of the impact patterns on the Vacuum Vessel produced by CX neutrals during the tangential balanced NBI in TJ-II Helical Axis Stellarator has been done. The results show periodical distributions with smooth maxima and mild loads, concentrated preferential on the HC plates. A certain preference of these neutral to emerge down wards from the plasma appears, as a consequence of a similar trend for the trapped particles. The differences between the impacts produced by the beam parallel to the magnetic field and the opposite one are small, once more as a consequence of the loss of memory of trapped particles to initial direction. The dependence of loads with plasma density and beam energy follows the trend of CX losses, decreasing strongly with increasing density and decreasing, more smoothly, with energy. (Author) 3 refs

  17. Impacts of lost fast ions on the TJ-II Vacuum vessel during NBI

    International Nuclear Information System (INIS)

    Guasp, J.

    1995-09-01

    The possible deposition patterns, on the Vacuum Vessel, of lost fast ions during the balanced tangential NBI in TJ-II helical axis Stellarator are analysed theoretically, establishing the relation between those impact points, the plasma exit and birth positions and positions and the magnetic configuration characteristics. It is shown that direct losses are the most important, mainly those produced by the beam injected with the same direction that the magnetic field, increasing with beam energy and plasma density but with impacts remaining fixed on well defined zones, a periodically distributed along the Hard Core cover plates, producing high loads at high densities. The remaining losses, except for the shine through ones that predominate at low density, are periodically distributed, with smooth maxima and produce very low loads. No overlapping between the different kind of losses or beams is observed

  18. 3D effects on transport and plasma control in the TJ-II stellarator

    Science.gov (United States)

    Castejón, F.; Alegre, D.; Alonso, A.; Alonso, J.; Ascasíbar, E.; Baciero, A.; de Bustos, A.; Baiao, D.; Barcala, J. M.; Blanco, E.; Borchardt, M.; Botija, J.; Cabrera, S.; de la Cal, E.; Calvo, I.; Cappa, A.; Carrasco, R.; Castro, R.; De Castro, A.; Catalán, G.; Chmyga, A. A.; Chamorro, M.; Dinklage, A.; Eliseev, L.; Estrada, T.; Fernández-Marina, F.; Fontdecaba, J. M.; García, L.; García-Cortés, I.; García-Gómez, R.; García-Regaña, J. M.; Guasp, J.; Hatzky, R.; Hernanz, J.; Hernández, J.; Herranz, J.; Hidalgo, C.; Hollmann, E.; Jiménez-Denche, A.; Kirpitchev, I.; Kleiber, R.; Komarov, A. D.; Kozachoek, A. S.; Krupnik, L.; Lapayese, F.; Liniers, M.; Liu, B.; López-Bruna, D.; López-Fraguas, A.; López-Miranda, B.; López-Razola, J.; Losada, U.; de la Luna, E.; Martín de Aguilera, A.; Martín-Díaz, F.; Martínez, M.; Martín-Gómez, G.; Martín-Hernández, F.; Martín-Rojo, A. B.; Martínez-Fernández, J.; McCarthy, K. J.; Medina, F.; Medrano, M.; Melón, L.; Melnikov, A. V.; Méndez, P.; Merino, R.; Miguel, F. J.; van Milligen, B.; Molinero, A.; Momo, B.; Monreal, P.; Moreno, R.; Navarro, M.; Narushima, Y.; Nedzelskiy, I. S.; Ochando, M. A.; Olivares, J.; Oyarzábal, E.; de Pablos, J. L.; Pacios, L.; Panadero, N.; Pastor, I.; Pedrosa, M. A.; de la Peña, A.; Pereira, A.; Petrov, A.; Petrov, S.; Portas, A. B.; Poveda, E.; Rattá, G. A.; Rincón, E.; Ríos, L.; Rodríguez, C.; Rojo, B.; Ros, A.; Sánchez, J.; Sánchez, M.; Sánchez, E.; Sánchez-Sarabia, E.; Sarksian, K.; Satake, S.; Sebastián, J. A.; Silva, C.; Solano, E. R.; Soleto, A.; Sun, B. J.; Tabarés, F. L.; Tafalla, D.; Tallents, S.; Tolkachev, A.; Vega, J.; Velasco, G.; Velasco, J. L.; Wolfers, G.; Yokoyama, M.; Zurro, B.

    2017-10-01

    The effects of 3D geometry are explored in TJ-II from two relevant points of view: neoclassical transport and modification of stability and dispersion relation of waves. Particle fuelling and impurity transport are studied considering the 3D transport properties, paying attention to both neoclassical transport and other possible mechanisms. The effects of the 3D magnetic topology on stability, confinement and Alfvén Eigenmodes properties are also explored, showing the possibility of controlling Alfvén modes by modifying the configuration; the onset of modes similar to geodesic acoustic modes are driven by fast electrons or fast ions; and the weak effect of magnetic well on confinement. Finally, we show innovative power exhaust scenarios using liquid metals.

  19. Pado, a fluorescent protein with proton channel activity can optically monitor membrane potential, intracellular pH, and map gap junctions.

    Science.gov (United States)

    Kang, Bok Eum; Baker, Bradley J

    2016-04-04

    An in silico search strategy was developed to identify potential voltage-sensing domains (VSD) for the development of genetically encoded voltage indicators (GEVIs). Using a conserved charge distribution in the S2 α-helix, a single in silico search yielded most voltage-sensing proteins including voltage-gated potassium channels, voltage-gated calcium channels, voltage-gated sodium channels, voltage-gated proton channels, and voltage-sensing phosphatases from organisms ranging from mammals to bacteria and plants. A GEVI utilizing the VSD from a voltage-gated proton channel identified from that search was able to optically report changes in membrane potential. In addition this sensor was capable of manipulating the internal pH while simultaneously reporting that change optically since it maintains the voltage-gated proton channel activity of the VSD. Biophysical characterization of this GEVI, Pado, demonstrated that the voltage-dependent signal was distinct from the pH-dependent signal and was dependent on the movement of the S4 α-helix. Further investigation into the mechanism of the voltage-dependent optical signal revealed that inhibiting the dimerization of the fluorescent protein greatly reduced the optical signal. Dimerization of the FP thereby enabled the movement of the S4 α-helix to mediate a fluorescent response.

  20. Josephson junctions array resonators

    Energy Technology Data Exchange (ETDEWEB)

    Gargiulo, Oscar; Muppalla, Phani; Mirzaei, Iman; Kirchmair, Gerhard [Institute for Quantum Optics and Quantum Information, Innsbruck (Austria)

    2016-07-01

    We present an experimental analysis of the self- and cross-Kerr effect of extended plasma resonances in Josephson junction chains. The chain consists of 1600 individual junctions and we can measure quality factors in excess of 10000. The Kerr effect manifests itself as a frequency shift that depends linearly on the number of photons in a resonant mode. By changing the input power we are able to measure this frequency shift on a single mode (self-kerr). By changing the input power on another mode while measuring the same one, we are able to evaluate the cross-kerr effect. We can measure the cross-Kerr effect by probing the resonance frequency of one mode while exciting another mode of the array with a microwave drive.

  1. Curved Josephson junction

    International Nuclear Information System (INIS)

    Dobrowolski, Tomasz

    2012-01-01

    The constant curvature one and quasi-one dimensional Josephson junction is considered. On the base of Maxwell equations, the sine–Gordon equation that describes an influence of curvature on the kink motion was obtained. It is showed that the method of geometrical reduction of the sine–Gordon model from three to lower dimensional manifold leads to an identical form of the sine–Gordon equation. - Highlights: ► The research on dynamics of the phase in a curved Josephson junction is performed. ► The geometrical reduction is applied to the sine–Gordon model. ► The results of geometrical reduction and the fundamental research are compared.

  2. Transport Calculations for the reference configuration under neutral bean injection in TJ-II

    International Nuclear Information System (INIS)

    Guasp, J.; Castejon, F.; Liniers, M.

    1999-01-01

    Transport calculations for the Reference Configuration under Neutral Beam Injection in TJ-II are discussed. For all these analysis the Transport Code PROCTR has been used but, in reason of the complex geometry of TJ-II, some modifications to the code have been needed, not only for the absorption, losses and deposition radial profile evaluations, but also for the treatment of the transition between ECRH and NBI or the fit of Transport Coefficients to the different Scaling Laws. The attained centralβ values for high density ( central value around 11 x 10''13 cm''3), in steady, range between a minimum of 1.9% for the GRB law up to 3.6% or 4.2% for those laws that show an explicit dependence with the rotational transform (ISS and LGS), with an intermediate value of 2.8% for the LHD case. Global energy confinement times range between 3.9 and 8.8 ms for the two extreme cases and 5.6 ms for LHD. As well ions as electrons are clearly in the plateau regime, in contrast to the ECRH phase where the electrons are well inside the 1/ν regime, dominated by helical ripple effects. The effect of impurities is to decrease slightly the absorption and the attainable β levels, but only for Zeff values higher than 4 this degradation becomes important. For the stationary state the density remains always below the semiempirical limit, independently of the Zeff value. Even along the first stages of injection, where absorption can be rather low, the limit is not reached, at least for Zeff < 4, so that radioactive collapse along this critical phase should not to be expected. (Author) 14 refs

  3. Design and testing of an electron Bernstein wave emission radiometer for the TJ-II Stellarator

    International Nuclear Information System (INIS)

    Caughman, J.B.O.; Rasmussen, D.A.; Carter, M.C.; Wilgen, J.B.; Cappa, A.; Castejon, F.; Fernandez, A.

    2005-01-01

    Efficient Electron Bernstein wave (EBW) mode conversion is important for heating dense plasmas in TJ-II. The O-X-B mode conversion scenario is being considered for heating plasmas with densities over 1,3 x 10 19 m -3 , which will be very interesting to study high-density physics and for heating NBI plasmas. Measurement of the thermal EBW emission from the plasma allows the EBW mode conversion efficiency to be determined, and also has the potential to offer a diagnostic for measuring electron temperature profile evolution in overdense plasmas. A dual-polarized quad-ridged broadband horn with a focusing lens will be used to measure the EBW emission at 28 GHz on TJ-II. A focused beam is needed to achieve efficient coupling at the mode conversion layer. Emission from the plasma is reflected from a steerable internal mirror, propagates through a glass lens, and is focused on the horn. The field pattern from the horn-lens combination has been measured as a function of horn-lens spacing and lens focal length with a 3-D scanning system in an effort to minimize the beam waist at the plasma edge. Beam waist sizes have been measured at distances of up to 80 cm from the lens. Details of the experimental results and future plans will be presented. [Oak Ridge National Laboratory is managed by UT-Battelle, LLC, for the U.S. Dept. of Energy under contract DE-AC05-00OR22725. A part of this work is performed under support of Spanish 'Subdireccion General de Proyectos de Investigacion, Ministerio de Educacion y Ciencia' with reference ENE2004-06957]. (author)

  4. Impact of gas puffing location on density control and plasma parameters in TJ-II

    International Nuclear Information System (INIS)

    Tabares, F.L.; Garcia-Cortes, I.; Estrada, T.; Tafalla, D.; Hidalgo, A.; Ferreira, J.A.; Pastor, I.; Herranz, J.; Ascasibar, E.

    2005-01-01

    Under pure Electron Cyclotron Resonance Heating (ECRH) conditions in TJ-II plasmas (P<300 kW, 53.2 GHz, 2nd harmonic X-mode, power density < 25 W/m''3), plasma start-up and good density control are obtained only by the proper combination of wall conditions and gas puffing characteristics. Such a control is particularly critical for the optimisation of the NBI power transfer to the target plasmas. The relatively low cut-off limit is easily reached due not only to the unfavourable wall/puffing-fuelling ratio but also due to the steep density profiles developed during the Enhanced Particle Confinement (EPC) modes. These modes are triggered by the gas puffing waveform, and they cannot be achieved for high iota magnetic configurations in TJ-II. Comparative experiments under metallic and boronised wall conditions have shown that the sensitivity of the EPC modes to the puffing rate is at least partially related to the energy balance at the plasma periphery under central heating scenarios. In this work, the impact of gas-fuelling location on the plasma parameters and density control is described. For that purpose, three different fuelling locations have been investigated; broad distribution from a side ports, localized injection from long tubes at different poloidal positions and highly localized injection through a movable limiter. Edge density and temperature profiles from a broad set of diagnostics (atomic beams, reflectometry, Thompson Scattering ECE, etc...) are analysed and compared. It has been found that preventing from transition to the EPC mode is achieved by using slow puffing rates, while neutral penetration into the plasma core can be enhanced for highly localized gas sources. Wall inventory, however, has been found to pl ay a dominant role in the fuelling of the plasma under most conditions. (author)

  5. Electron internal transport barriers and magnetic topology in the stellarator TJ-II

    International Nuclear Information System (INIS)

    Estrada, T.; Alonso, A.; Castejon, F.; Hidalgo, C.; Pablos, J.L. de; Tereshin, V.; Krupnik, L.; Chmyga, A.A.; Dreval, N.; Khrebtov, S.M.; Komarov, A.D.; Kozachok, A.S.; Eliseev, L.; Melnikov, A.V.

    2005-01-01

    Electron Internal Transport Barriers (e-ITBs) are frequently observed in helical systems. e-ITBs are characterized by an increase in core electron temperature and plasma potential as well as an improvement in core electron heat confinement. A comparative study of transport barriers in different helical devices will be presented by Yokoyama et al at this conference. In most helical systems, and in particular in TJ-II stellarator, the formation of e-ITBs is observed in Electron Cyclotron Heated plasmas with high heating power density. In TJ-II, e-ITBs are also formed in magnetic configurations having a low order rational surface close to the plasma core where the ECH power is deposited. In such configurations the key element to improve heat confinement, i.e. the strong radial electric field, results from a synergistic effect between enhanced electron heat fluxes through the low order rational surface and pump-out mechanisms in the heat deposition zone. Recent experiments show a quasi-coherent mode associated with a rational surface that triggers the formation of the e-ITB. This quasi-coherent mode is observed by both ECE and HIBP diagnostics. The mode is found to be localized within the radial range ρ: 0.0 - 0.4, with a maximum amplitude around ρ: 0.25 - 0.35, close to the foot of the e- ITB. The quasi-coherent mode evolves during the formation/annihilation of the e-ITB and vanishes as the barrier is fully developed. These observations indicate that the quasi-coherent modes are modified by the radial electric fields that develop at the transitions, thereby showing the importance of ExB flows in the evolution of MHD instabilities linked to low-order rational surfaces. Further studies are in progress to investigate the influence of the order of the low rational surfaces (3/2, 5/3,...) in triggering core transitions. (author)

  6. Anchored PKA as a gatekeeper for gap junctions.

    Science.gov (United States)

    Pidoux, Guillaume; Taskén, Kjetil

    2015-01-01

    Anchored protein kinase A (PKA) bound to A Kinase Anchoring Protein (AKAP) mediates effects of localized increases in cAMP in defined subcellular microdomains and retains the specificity in cAMP-PKA signaling to distinct extracellular stimuli. Gap junctions are pores between adjacent cells constituted by connexin proteins that provide means of communication and transfer of small molecules. While the PKA signaling is known to promote human trophoblast cell fusion, the gap junction communication through connexin 43 (Cx43) is a prerequisite for this process. We recently demonstrated that trophoblast fusion is regulated by ezrin, a known AKAP, which binds to Cx43 and delivers PKA in the vicinity gap junctions. We found that disruption of the ezrin-Cx43 interaction abolished PKA-dependent phosphorylation of Cx43 as well as gap junction communication and subsequently cell fusion. We propose that the PKA-ezrin-Cx43 macromolecular complex regulating gap junction communication constitutes a general mechanism to control opening of Cx43 gap junctions by phosphorylation in response to cAMP signaling in various cell types.

  7. Protein: FBA7 [TP Atlas

    Lifescience Database Archive (English)

    Full Text Available FBA7 claudin-zona occluden Tjp1 Zo1 Tight junction protein ZO-1 Tight junction protein 1, Zona occludens pr...otein 1, Zonula occludens protein 1 10090 Mus musculus 21872 P39447 2RRM P39447 21431884 ...

  8. Lipid rafts regulate PCB153-induced disruption of occludin and brain endothelial barrier function through protein phosphatase 2A and matrix metalloproteinase-2

    Energy Technology Data Exchange (ETDEWEB)

    Eum, Sung Yong, E-mail: seum@miami.edu; Jaraki, Dima; András, Ibolya E.; Toborek, Michal

    2015-09-15

    Occludin is an essential integral transmembrane protein regulating tight junction (TJ) integrity in brain endothelial cells. Phosphorylation of occludin is associated with its localization to TJ sites and incorporation into intact TJ assembly. The present study is focused on the role of lipid rafts in polychlorinated biphenyl (PCB)-induced disruption of occludin and endothelial barrier function. Exposure of human brain endothelial cells to 2,2′,4,4′,5,5′-hexachlorobiphenyl (PCB153) induced dephosphorylation of threonine residues of occludin and displacement of occludin from detergent-resistant membrane (DRM)/lipid raft fractions within 1 h. Moreover, lipid rafts modulated the reduction of occludin level through activation of matrix metalloproteinase 2 (MMP-2) after 24 h PCB153 treatment. Inhibition of protein phosphatase 2A (PP2A) activity by okadaic acid or fostriecin markedly protected against PCB153-induced displacement of occludin and increased permeability of endothelial cells. The implication of lipid rafts and PP2A signaling in these processes was further defined by co-immunoprecipitation of occludin with PP2A and caveolin-1, a marker protein of lipid rafts. Indeed, a significant MMP-2 activity was observed in lipid rafts and was increased by exposure to PCB153. The pretreatment of MMP-2 inhibitors protected against PCB153-induced loss of occludin and disruption of lipid raft structure prevented the increase of endothelial permeability. Overall, these results indicate that lipid raft-associated processes, such as PP2A and MMP-2 activation, participate in PCB153-induced disruption of occludin function in brain endothelial barrier. This study contributes to a better understanding of the mechanisms leading to brain endothelial barrier dysfunction in response to exposure to environmental pollutants, such as ortho-substituted PCBs. - Highlights: • PCB153 disturbed human brain endothelial barrier through disruption of occludin. • Lipid raft-associated PP

  9. Virus interaction with the apical junctional complex.

    Science.gov (United States)

    Gonzalez-Mariscal, Lorenza; Garay, Erika; Lechuga, Susana

    2009-01-01

    In order to infect pathogens must breach the epithelial barriers that separate the organism from the external environment or that cover the internal cavities and ducts of the body. Epithelia seal the passage through the paracellular pathway with the apical junctional complex integrated by tight and adherens junctions. In this review we describe how viruses like coxsackie, swine vesicular disease virus, adenovirus, reovirus, feline calcivirus, herpes viruses 1 and 2, pseudorabies, bovine herpes virus 1, poliovirus and hepatitis C use as cellular receptors integral proteins present at the AJC of epithelial cells. Interaction with these proteins contributes in a significant manner in defining the particular tropism of each virus. Besides these proteins, viruses exhibit a wide range of cellular co-receptors among which proteins present in the basolateral cell surface like integrins are often found. Therefore targeting proteins of the AJC constitutes a strategy that might allow viruses to bypass the physical barrier that blocks their access to receptors expressed on the basolateral surface of epithelial cells.

  10. The human myotendinous junction

    DEFF Research Database (Denmark)

    Knudsen, A B; Larsen, M; Mackey, Abigail

    2015-01-01

    The myotendinous junction (MTJ) is a specialized structure in the musculotendinous system, where force is transmitted from muscle to tendon. Animal models have shown that the MTJ takes form of tendon finger-like processes merging with muscle tissue. The human MTJ is largely unknown and has never...... been described in three dimensions (3D). The aim of this study was to describe the ultrastructure of the human MTJ and render 3D reconstructions. Fourteen subjects (age 25 ± 3 years) with isolated injury of the anterior cruciate ligament (ACL), scheduled for reconstruction with a semitendinosus...

  11. Charge Transport Phenomena in Peptide Molecular Junctions

    International Nuclear Information System (INIS)

    Luchini, A.; Petricoin, E.F.; Geho, D.H.; Liotta, L.A.; Long, D.P.; Vaisman, I.I.

    2008-01-01

    Inelastic electron tunneling spectroscopy (IETS) is a valuable in situ spectroscopic analysis technique that provides a direct portrait of the electron transport properties of a molecular species. In the past, IETS has been applied to small molecules. Using self-assembled nano electronic junctions, IETS was performed for the first time on a large polypeptide protein peptide in the phosphorylated and native form, yielding interpretable spectra. A reproducible 10-fold shift of the I/V characteristics of the peptide was observed upon phosphorylation. Phosphorylation can be utilized as a site-specific modification to alter peptide structure and thereby influence electron transport in peptide molecular junctions. It is envisioned that kinases and phosphatases may be used to create tunable systems for molecular electronics applications, such as biosensors and memory devices.

  12. Junctional epidermolysis bullosa(non-herlitz type)

    International Nuclear Information System (INIS)

    Bhinder, M. A.; Arshad, M. W.; Shabbir, M. I.; Zahoor, M. Y.; Shehzad, W.; Tariq, M.

    2017-01-01

    Junctional epidermolysis bullosa (JEB) is a recessively inherited skin blistering disease and is caused due to abnormalities in proteins that hold layers of the skin. Herlitz JEB is the severe form and non-Herlitz JEB is the milder form. This report describes a case of congenitally affected male child aged 5 years, with skin blistering. He has mitten-like hands and soft skin blistering on hands, legs and knees. Symptoms almost disappeared at the age of 3 years but reappeared with increased severity after 6 months. Histopathological examination showed epidermal detachment with intact basal cell layer and sparse infiltrate of lymphocytes with few eosinophils in the dermis. There was no blistering on the moist lining of the mouth and digestive tract. Localized symptoms with less lethality and histopathological examination indicated the presence of non-Herlitz type of JEB. This is the first report which confirms the presence of non-Herlitz junctional epidermolysis bullosa in Pakistan. (author)

  13. Junctional Epidermolysis Bullosa (Non-Herlitz Type).

    Science.gov (United States)

    Bhinder, Munir Ahmad; Arshad, Muhammad Waqar; Zahoor, Muhammad Yasir; Shehzad, Wasim; Tariq, Muhammad; Shabbir, Muhammad Imran

    2017-05-01

    Junctional epidermolysis bullosa (JEB) is a recessively inherited skin blistering disease and is caused due to abnormalities in proteins that hold layers of the skin. Herlitz JEB is the severe form and non-Herlitz JEB is the milder form. This report describes a case of congenitally affected male child aged 5 years, with skin blistering. He has mitten-like hands and soft skin blistering on hands, legs and knees. Symptoms almost disappeared at the age of 3 years but reappeared with increased severity after 6 months. Histopathological examination showed epidermal detachment with intact basal cell layer and sparse infiltrate of lymphocytes with few eosinophils in the dermis. There was no blistering on the moist lining of the mouth and digestive tract. Localized symptoms with less lethality and histopathological examination indicated the presence of non-Herlitz type of JEB. This is the first report which confirms the presence of non-Herlitz junctional epidermolysis bullosa in Pakistan.

  14. Cell junction protein armadillo repeat gene deleted in velo-cardio-facial syndrome is expressed in the skin and colocalizes with autoantibodies of patients affected by a new variant of endemic pemphigus foliaceus in Colombia.

    Science.gov (United States)

    Abreu-Velez, Ana Maria; Yi, Hong; Howard, Michael S

    2017-10-01

    We previously described a new variant of endemic pemphigus foliaceus in El Bagre, Colombia, South America (El Bagre-EPF, or pemphigus Abreu-Manu). El Bagre-EPF differs from other types of EPF clinically, epidemiologically, immunologically and in its target antigens. We reported the presence of patient autoantibodies colocalizing with armadillo repeat gene deleted in velo-cardio-facial syndrome (ARVCF), a catenin cell junction protein colocalizing with El Bagre-EPF autoantibodies in the heart and within pilosebaceous units along their neurovascular supply routes. Here we investigate the presence of ARVCF in skin and its possibility as a cutaneous El Bagre-EPF antigen. We used a case-control study, testing sera of 45 patients and 45 controls via direct and indirect immunofluorescence (DIF/IIF), confocal microscopy, immunoelectron microscopy and immunoblotting for the presence of ARVCF and its relationship with El Bagre-EPF autoantibodies in the skin. We also immunoadsorbed samples with desmoglein 1 (Dsg1) ectodomain (El Bagre-EPF antigen) by incubating with the positive ARVCF samples from DIF and IIF. ARVCF was expressed in all the samples from the cases and controls. Immunoadsorption with Dsg1 on positive ARVCF immunofluorescence DIF/IIF cases showed that the immune response was present against non-desmoglein 1 antigen(s). Overall, 40/45 patients showed colocalization of their autoantibodies with ARVCF in the epidermis; no controls from the endemic area displayed colocalization. We demonstrate that ARVCF is expressed in many areas of human skin, and colocalizes with the majority of El Bagre-EPF autoantibodies as a putative antigen.

  15. Dynamical Simulation of Recycling and Particle Fueling in TJ-II Plasmas; Simulacion Dinamica del Reciclado y de la Inyeccion de Particulas en los Plasmas del TJ-II

    Energy Technology Data Exchange (ETDEWEB)

    Lopez-Bruna, D; Ferreira, J A; Tabares, F L; Castejon, F; Guasp, J

    2007-07-20

    With the aim of improving the calculation tools for transport analysis in TJ-II plasmas, in this work we analyze the simplified model for a kinetic equation that ASTRA uses to calculate the neutral particle distribution in the plasma. Next, we act on the boundary conditions for this kinetic equation (particularly on the neutral density in the plasma boundary) so we can simulate the recycling conditions for the TJ-II in a simple way. With the resulting transport models we can easily analyze the sensibility of these plasmas to the cold gas puffing depending on the recycling conditions. These transport models evidence the problem of density control in the TJ-II. Likewise, we estimate the importance of recycling in the plasmas heated by energetic neutral beam injection. The experimentally observed increments in density when the energetic neutrals are injected would respond, according to the calculations here presented, to a large increment of the neutrals influx that cannot be explained by the beam itself. (Author) 22 refs.

  16. Effect of fast electrons on the stability of resistive interchange modes in the TJ-II stellarator

    Energy Technology Data Exchange (ETDEWEB)

    García, L. [Universidad Carlos III de Madrid, Avda. de la Universidad 30, 28911 Leganés, Madrid (Spain); Ochando, M. A.; Hidalgo, C.; Milligen, B. Ph. van [CIEMAT - Laboratorio Nacional de Fusión, Avda. Complutense 40, 28040 Madrid (Spain); Carreras, B. A. [BACV Solutions, 110 Mohawk Road, Oak Ridge, Tennessee 37830 (United States); Carralero, D. [Max-Planck-Institute for Plasma Physics, Boltzmannstr. 2, Garching (Germany)

    2016-06-15

    In this paper, we report on electromagnetic phenomena in low-β plasmas at the TJ-II stellarator, controlled by external heating. To understand the observations qualitatively, we introduce a simple modification of the standard resistive MHD equations, to include the potential impact of fast electrons on instabilities. The dominant instabilities of the modeling regime are resistive interchange modes, and calculations are performed in a configuration with similar characteristics as the TJ-II stellarator. The main effect of the trapping of fast electrons by magnetic islands induced by MHD instabilities is to increase the magnetic component of the fluctuations, changing the character of the instability to tearing-like and modifying the frequency of the modes. These effects seem to be consistent with some of the experimental observations.

  17. Transitions to improved core electron heat confinement triggered by low order rational magnetic surfaces in the stellarator TJ-II

    International Nuclear Information System (INIS)

    Estrada, T.; Medina, F.; Lopez-Bruna, D.; AscasIbar, E.; BalbIn, R.; Cappa, A.; Castejon, F.; Eguilior, S.; Fernandez, A.; Guasp, J.; Hidalgo, C.; Petrov, S.

    2007-01-01

    Transitions to improved core electron heat confinement are triggered by low order rational magnetic surfaces in TJ-II electron cyclotron heated (ECH) plasmas. Experiments are performed changing the magnetic shear around the rational surface n = 3/m = 2 to study its influence on the transition; ECH power modulation is used to look at transport properties. The improvement in the electron heat confinement shows no obvious dependence on the magnetic shear. Transitions triggered by the rational surface n = 4/m = 2 show, in addition, an increase in the ion temperature synchronized with the increase in the electron temperature. Ion temperature changes had not been previously observed either in TJ-II or in any other helical device. SXR measurements demonstrate that, under certain circumstances, the rational surface positioned inside the plasma core region precedes and provides a trigger for the transition

  18. Företagens behov av resebyråtjänster : Case: Karleby Resebyrå Ab

    OpenAIRE

    Björkroth, Pinja

    2016-01-01

    Syftet med arbetet är att utreda orsaker till varför resebyråtjänster används samt utreda vilka andra möjliga behov företagen har gällande resebyråtjänster. Undersökningen görs till Karleby Resebyrå. I den teoretiska delen behandlas marknadsmixerna 4P, 4C och 7P, business to business och kundlojaliteten. Som forskningsmetod i arbetet användes en kvantitativ metod med hjälp av en webbenkät. Enkäten består av 21 frågor varav 11 är öppna frågor. Webbenkäten skickades per e-post till 50 föret...

  19. Claudin-2 knockout by TALEN-mediated gene targeting in MDCK cells: claudin-2 independently determines the leaky property of tight junctions in MDCK cells.

    Directory of Open Access Journals (Sweden)

    Shinsaku Tokuda

    Full Text Available Tight junctions (TJs regulate the movements of substances through the paracellular pathway, and claudins are major determinants of TJ permeability. Claudin-2 forms high conductive cation pores in TJs. The suppression of claudin-2 expression by RNA interference in Madin-Darby canine kidney (MDCK II cells (a low-resistance strain of MDCK cells was shown to induce a three-fold increase in transepithelial electrical resistance (TER, which, however, was still lower than in high-resistance strains of MDCK cells. Because RNA interference-mediated knockdown is not complete and only reduces gene function, we considered the possibility that the remaining claudin-2 expression in the knockdown study caused the lower TER in claudin-2 knockdown cells. Therefore, we investigated the effects of claudin-2 knockout in MDCK II cells by establishing claudin-2 knockout clones using transcription activator-like effector nucleases (TALENs, a recently developed genome editing method for gene knockout. Surprisingly, claudin-2 knockout increased TER by more than 50-fold in MDCK II cells, and TER values in these cells (3000-4000 Ω·cm2 were comparable to those in the high-resistance strains of MDCK cells. Claudin-2 re-expression restored the TER of claudin-2 knockout cells dependent upon claudin-2 protein levels. In addition, we investigated the localization of claudin-1, -2, -3, -4, and -7 at TJs between control MDCK cells and their respective knockout cells using their TALENs. Claudin-2 and -7 were less efficiently localized at TJs between control and their knockout cells. Our results indicate that claudin-2 independently determines the 'leaky' property of TJs in MDCK II cells and suggest the importance of knockout analysis in cultured cells.

  20. Electrostatic and magnetic fluctuations in the proximity of the velocity shear layer in the TJ-I Tokamak

    International Nuclear Information System (INIS)

    Garcia-Cortes, I.; Pedrosa, M.A.; Hidalgo, C.

    1992-01-01

    The structure of the electrostatic and magnetic turbulence changes in the proximity of the naturally velocity shear layer in the TJ-I tokamak. A decorrelation in the broad-band magnetic fluctuations and a decreasing in the density fluctuation levels have been observed in the proximity (scrape-off layer side) of the shear layer. The results are interpreted in terms of turbulence characteristics modified by sheared poloidal flows or/and magnetic configuration. (author) 8 fig. 16 ref

  1. Characterization of the Tetraspan Junctional Complex (4JC) superfamily.

    Science.gov (United States)

    Chou, Amy; Lee, Andre; Hendargo, Kevin J; Reddy, Vamsee S; Shlykov, Maksim A; Kuppusamykrishnan, Harikrishnan; Medrano-Soto, Arturo; Saier, Milton H

    2017-03-01

    Connexins or innexins form gap junctions, while claudins and occludins form tight junctions. In this study, statistical data, derived using novel software, indicate that these four junctional protein families and eleven other families of channel and channel auxiliary proteins are related by common descent and comprise the Tetraspan (4 TMS) Junctional Complex (4JC) Superfamily. These proteins all share similar 4 transmembrane α-helical (TMS) topologies. Evidence is presented that they arose via an intragenic duplication event, whereby a 2 TMS-encoding genetic element duplicated tandemly to give 4 TMS proteins. In cases where high resolution structural data were available, the conclusion of homology was supported by conducting structural comparisons. Phylogenetic trees reveal the probable relationships of these 15 families to each other. Long homologues containing fusions to other recognizable domains as well as internally duplicated or fused domains are reported. Large "fusion" proteins containing 4JC domains proved to fall predominantly into family-specific patterns as follows: (1) the 4JC domain was N-terminal; (2) the 4JC domain was C-terminal; (3) the 4JC domain was duplicated or occasionally triplicated and (4) mixed fusion types were present. Our observations provide insight into the evolutionary origins and subfunctions of these proteins as well as guides concerning their structural and functional relationships. Copyright © 2016 Elsevier B.V. All rights reserved.

  2. Effects of the radial electrical field on the drifts, trapping and particle orbits in TJ-II

    International Nuclear Information System (INIS)

    Guasp, J.; Liniers, M.

    1997-01-01

    In this study a detailed analysis of the effect of radial electric fields on drifts, trapping and trajectories for ions of low and intermediate energy (0.1-1 keV) in the helical axis stellarator TJ-II has been performed. In TJ-II the drift velocities have the same rotation direction than the Hard Core (HC, the same than the plasma) with predominance of the vertical downwards component. The intensity is higher near the HC and in the outwards direction. These trends create strong asymmetries in losses even in the absence of electric field. When an electric field is present the poloidal components of the drift velocity predominates modifying deeply the orbit behaviour. Positive electric fields produce internal radial trapping barriers and have a tendency to eliminate the external ones. The opposite happens for negative fields. These facts alterate deeply the tapping and confinement properties of the particles. All these analysis will be used as a basis for the understanding of the modifications on the loss distribution, trapping regions and loss cones for TJ-II that will be addressed in forthcoming studies. (Author)

  3. Software and hardware developments for remote participation in TJ-II operation. Proof of concept using the NPA diagnostic

    International Nuclear Information System (INIS)

    Lopez, A.; Vega, J.; Montoro, A.; Sanchez, E.; Encabo, J.; Portas, A.; Balbin, R.; Fontdecaba, J.M.; Jimenez, J.A.; Dies, J.

    2002-01-01

    The operation protocols for the TJ-II require that all diagnostics be controlled remotely during machine operation. For this reason, most diagnostics have control systems that permit remote actions to be performed on their various subsystems. An Internet browser, which connects to a server where the human machine interfaces (HMIs) for all diagnostics are situated, is used for this. Real time control has been resolved using programmable automates for each diagnostic. Dedicated application software is in operation to provide a user interface for programming digitisers, for signal visualisation and for data processing during TJ-II discharges. This software is an event-based application that can be remotely launched from any X terminal. Other resources provided to users are graphical and computational tools for data analysis, data compression, off-line access to databases, reception of data and its integration in the databases at any moment. Two neutral particle analysers (NPAs) are operating on TJ-II to measure the energy spectra of charge exchange (CX) neutral particles. Protocols require remote control over several actions and subsystems of this diagnostic. With all these tools a proof of the concept of remote participation has being performed during the present experimental campaign. The NPA diagnostic has been operated from UPC-ETSEIB in Barcelona, 600 km of distance from the machine

  4. Junction detection and pathway selection

    Science.gov (United States)

    Peck, Alex N.; Lim, Willie Y.; Breul, Harry T.

    1992-02-01

    The ability to detect junctions and make choices among the possible pathways is important for autonomous navigation. In our script-based navigation approach where a journey is specified as a script of high-level instructions, actions are frequently referenced to junctions, e.g., `turn left at the intersection.' In order for the robot to carry out these kind of instructions, it must be able (1) to detect an intersection (i.e., an intersection of pathways), (2) know that there are several possible pathways it can take, and (3) pick the pathway consistent with the high level instruction. In this paper we describe our implementation of the ability to detect junctions in an indoor environment, such as corners, T-junctions and intersections, using sonar. Our approach uses a combination of partial scan of the local environment and recognition of sonar signatures of certain features of the junctions. In the case where the environment is known, we use additional sensor information (such as compass bearings) to help recognize the specific junction. In general, once a junction is detected and its type known, the number of possible pathways can be deduced and the correct pathway selected. Then the appropriate behavior for negotiating the junction is activated.

  5. Mixing in T-junctions

    NARCIS (Netherlands)

    Kok, Jacobus B.W.; van der Wal, S.

    1996-01-01

    The transport processes that are involved in the mixing of two gases in a T-junction mixer are investigated. The turbulent flow field is calculated for the T-junction with the k- turbulence model by FLOW3D. In the mathematical model the transport of species is described with a mixture fraction

  6. Dynamics of Josephson junction arrays

    International Nuclear Information System (INIS)

    Hadley, P.

    1989-01-01

    The dynamics of Josephson junction arrays is a topic that lies at the intersection of the fields of nonlinear dynamics and Josephson junction technology. The series arrays considered here consist of several rapidly oscillating Josephson junctions where each junction is coupled equally to every other junction. The purpose of this study is to understand phaselocking and other cooperative dynamics of this system. Previously, little was known about high dimensional nonlinear systems of this sort. Numerical simulations are used to study the dynamics of these arrays. Three distinct types of periodic solutions to the array equations were observed as well as period doubled and chaotic solutions. One of the periodic solutions is the symmetric, in-phase solution where all of the junctions oscillate identically. The other two periodic solutions are symmetry-broken solutions where all of the junction do not oscillate identically. The symmetry-broken solutions are highly degenerate. As many as (N - 1) stable solutions can coexist for an array of N junctions. Understanding the stability of these several solutions and the transitions among them is vital to the design of useful devices

  7. Effects of the Electric Field on the Direct Loss Asymmetries on TJ-II

    International Nuclear Information System (INIS)

    Guasp, J.

    1997-01-01

    The effects of the radial electric fields upon the direct loss asymmetries have been studied for low energy (0.1-1keV)ions. The analysis shows the permanence of the predominant downwards asymmetry that is only slightly modified in the poloidal rotation direction for moderate electric fields. In TJ-II this poloidal rotation is directed towards decreasing poloidal angles for positive (outward) electric fields and the ions drift along the same direction. Nevertheless for very strong fields the lost particles concentrate in the extreme plasma periphery (at the same time that the rate loss decreases strongly) and have no time, any more, to feel the poloidal rotation before escaping. The gaps between the TF coils receive the most of the losses. On the Vacuum Vessel the losses have a very strong concentration along the Hard Core, in particular on the PLT-2 plane plate (the one that is placed down at Phi=0 degree centigree) and a moderate preference for the 2''nd toroidal octant of each period. The poloidal rotation is much less visible than on the plasma border. The resulting power loads are small. (Author)

  8. The structure and dynamics of blob filaments in the stellarator TJ-K

    Energy Technology Data Exchange (ETDEWEB)

    Garland, Stephen; Ramisch, Mirko [Institut fuer Grenzflaechenverfahrenstechnik und Plasmatechnologie, Universitaet Stuttgart (Germany); Fuchert, Golo [Max-Planck-Institut fuer Plasmaphysik, Greifswald (Germany)

    2016-07-01

    Filamental structures with higher pressure than the background plasma are commonly observed in the scrape-off layer (SOL) of toroidal magnetic confinement devices. These structures, often referred to as blobs, propagate radially outwards and poloidally, contributing significantly to SOL transport. It is therefore important to study the properties of blobs in order to be able to predict heat loads on the plasma facing components of future reactors, as well as to better understand particle transport and plasma confinement. Detailed experiments have been carried out into blob dynamics and structure using Langmuir probes at the stellarator TJ-K. By means of the conditional averaging technique, blob dynamics in a poloidal cross section have been studied, and the influence of geodesic curvature on poloidal blob drive are shown. In addition, the result of simultaneous measurements at two toroidally separated locations is presented, providing information on the 3D structure of blob filaments and their alignment to the magnetic field as they propagate through the SOL.

  9. Edge and Plasma -Wall Interaction Diagnostics in the TJ-II Stellarator

    Energy Technology Data Exchange (ETDEWEB)

    Tabares, F. L.; Tafalla, D.; Branas, B.; Hidalgo, A.; Garcia-Cortes, I.; Lopez-Fraguas, A.; Ortiz, P.

    2003-07-01

    The operation of the TJ-II stellarator, carried out under ECR heating conditions until now, the plasma edge parameters and those processes has been identified. Therefore, an important , has implieda careful control of partied e sources and the associated plasma-wall interaction processes. A clear coupling between the plasma edge parameters and those processes has been identified. Therefore, an important effort has been devoted to the development of dedicated diagnostics in both fields. Remarkable success has been attained in the development of atomic-beam based edge diagnostics, namely, thermal Li and supersonic He beams. In particular, fast (up to 200 Hz) sampling of temperature and density profiles has been made possible thorough an upgraded version of the pulsed, supersonic He beam diagnostic. In this paper, whorl devoted to the upgrading of these techniques is described. Also, preliminary experiments oriented to the validation of the collisional radiative models use din the beam-based diagnostic interpretaron as well as simulations of Laser Induced Fluorescence (LIF) studies of level populations of electronically excited He atoms are shown. (Author) 17 refs.

  10. Flat-histogram methods in quantum Monte Carlo simulations: Application to the t-J model

    International Nuclear Information System (INIS)

    Diamantis, Nikolaos G.; Manousakis, Efstratios

    2016-01-01

    We discuss that flat-histogram techniques can be appropriately applied in the sampling of quantum Monte Carlo simulation in order to improve the statistical quality of the results at long imaginary time or low excitation energy. Typical imaginary-time correlation functions calculated in quantum Monte Carlo are subject to exponentially growing errors as the range of imaginary time grows and this smears the information on the low energy excitations. We show that we can extract the low energy physics by modifying the Monte Carlo sampling technique to one in which configurations which contribute to making the histogram of certain quantities flat are promoted. We apply the diagrammatic Monte Carlo (diag-MC) method to the motion of a single hole in the t-J model and we show that the implementation of flat-histogram techniques allows us to calculate the Green's function in a wide range of imaginary-time. In addition, we show that applying the flat-histogram technique alleviates the “sign”-problem associated with the simulation of the single-hole Green's function at long imaginary time. (paper)

  11. Characterization of the quasi-coherent oscillations by HIBP diagnostic in the TJ-II stellarator

    International Nuclear Information System (INIS)

    Krupnik, L.; Chmyga, A.A.; Dreval, N.; Khrebtov, S.M.; Komarov, A.D.; Kozachok, A.S.; Eliseev, L.; Melnikov, A.; Perfilov, S.V.; Alonso, A.; Pablos, J.L. de; Hidalgo, C.; Pedrosa, M.A.

    2005-01-01

    Quasicoherent oscillations have been observed in TJ-II plasma with different diagnostic. A recent improvement in the signal to noise ratio of the Heavy Ion Beam Probe (HIBP) diagnostic has allowed to observe the radial structure of these oscillations from the edge to the plasma core region. Edge quasi-coherent fluctuations (with frequencies near 20 kHz) have been observed in some configuration windows when plasma density / heating power are above a threshold. The amplitude of those modes tends to be larger in the low field side region. This result suggests the role of configuration (related to the presence of low order rationals in the plasma edge) and threshold gradients to trigger quasi-coherence modes. HIBP signals are strongly correlated with probe signals. When rationals move towards the plasma core (ρ ∼ 0.3), the modes are clearly seen in ECE emission and in HIBP secondary current and potential signals. These quasi-coherent oscillations (in range 20 kHz) have been connected with the development of electron internal transport barriers (e-ITB). Recent results show a decreasing in the mode amplitude as e-ITBs are fully developed. (author)

  12. Dependence of direct losses and trapping properties with the magnetic configuration in TJ-II

    International Nuclear Information System (INIS)

    Guasp, J.; Liniers, M.

    1998-05-01

    The former studies concerning direct losses, disymmetries, trapping and radial electric field effects for intermediate energy ions have been extended to several magnetic configurations in TJ-II. In the absence of electric field there are strong similarities in the behaviour of all configurations: disymmetries, loss distributions at plasma border, radial and angular profiles, etc. Generally the differences are only quantitative and dominated by the magnetic ripple at border, that is clearly related with the configuration radius. This qualitative similarity disappears in the presence of a radial electric field. The field resonance are at the origin of these differences. A simple model reproduces correctly the ordering and degree of influence of these resonances. Except when the 0 resonance predominates the los distributions at plasma border move always in the direction of the induced poloidal rotation. The los radial profiles are strongly affected by the -2 Resonance, that can provoke the appearance of lost passing ions well inside the plasma. Instead the radial and angular profiles for trapping are only slightly affected by the -2 Resonance, while the 0 Resonance has a very strong influence there

  13. Influence of Plasma Biasing on Coherent Structures in TJ-K

    Science.gov (United States)

    Ramisch, M.; Greiner, F.; Lechte, C.; Mahdizadeh, N.; Rahbarnia, K.; Stroth, U.

    2003-10-01

    Poloidal shear flows play an important role in the improvement of plasma confinement in fusion devices. They limit the radial correlation length via the shear decorrelation mechanism [1] and can trigger transitions into transport barriers. External biasing can be used to drive poloidal shear flows [2] in order to study the decorrelation mechanism. The torsatron TJ-K is operated with low-temperature plasmas produced by ECRH. Coherent and quasi-coherent structures have been observed [3]. Their structure size varies according to the drift scale ρ_s. The influence of biasing on these structures is investigated by means of electrostatic probes. Electron density fluctuations as well as fluctuations of the floating potential tend to decrease in the presence of a positively biased probe. The evolution of radial electric field, poloidal flow and radial transport are investigated for different plasma parameters using a 2D Langmuir probe array with 64 tips in comparison with two-point correlation measurements. First results are presented. [1] H. Biglari et al., Phys. Fluids B 2, p. 1 (1990); [2] R. J. Taylor et al., Phys. Rev. Lett. 63, 21, p. 2365 (1989); [3] C. Lechte, PhD-Thesis, CAU Kiel (2003)

  14. Distributed real time data processing architecture for the TJ-II data acquisition system

    International Nuclear Information System (INIS)

    Ruiz, M.; Barrera, E.; Lopez, S.; Machon, D.; Vega, J.; Sanchez, E.

    2004-01-01

    This article describes the performance of a new model of architecture that has been developed for the TJ-II data acquisition system in order to increase its real time data processing capabilities. The current model consists of several compact PCI extension for instrumentation (PXI) standard chassis, each one with various digitizers. In this architecture, the data processing capability is restricted to the PXI controller's own performance. The controller must share its CPU resources between the data processing and the data acquisition tasks. In the new model, distributed data processing architecture has been developed. The solution adds one or more processing cards to each PXI chassis. This way it is possible to plan how to distribute the data processing of all acquired signals among the processing cards and the available resources of the PXI controller. This model allows scalability of the system. More or less processing cards can be added based on the requirements of the system. The processing algorithms are implemented in LabVIEW (from National Instruments), providing efficiency and time-saving application development when compared with other efficient solutions

  15. Edge and Plasma-Wall Interaction Diagnostics in the TJ-II Stellarator

    International Nuclear Information System (INIS)

    Tabares, F.L.; Tafalla, D.; Branas, B.; Hidalgo, A.; Garcia-Cortes, I.; Lopez-Fraguas, A.; Ortiz, P.

    2003-01-01

    The operation of the TJ-II stellarator, carried out under ECR heating conditions until now, the plasma edge parameters and those processes has been identified. Therefore, an important, has implied a careful control of partied e sources and the associated plasma-wall interaction processes. A clear coupling between the plasma edge parameters and those processes has been identified. Therefore, an important effort has been devoted to the development of dedicated diagnostics in both fields. Remarkable success has been attained in the development of atomic-beam based edge diagnostics, namely, thermal Li and supersonic He beams. In particular, fast (up to 200 Hz) sampling of temperature and density profiles has been made possible thorough an upgraded version of the pulsed, supersonic He beam diagnostic. In this paper, whorl devoted to the upgrading of these techniques is described. Also, preliminary experiments oriented to the validation of the collisional radiative models used in the beam-based diagnostic interpretaron as well as simulations of Laser Induced Fluorescence (LIF) studies of level populations of electronically excited He atoms are shown. (Author) 17 refs

  16. Trichomonas vaginalis perturbs the junctional complex in epithelial cells

    Institute of Scientific and Technical Information of China (English)

    2005-01-01

    Trichomonas vaginalis, a protist parasite of the urogenital tract in humans, is the causative agent of trichomonosis,which in recent years have been associated with the cervical cancer development. In the present study we analyzed the modifications at the junctional complex level of Caco-2 cells after interaction with two isolates of T. vaginalis and the influence of the iron concentration present in the parasite's culture medium on the interaction effects. Our results show that T. vaginalis adheres to the epithelial cell causing alterations in the junctional complex, such as: (a) a decrease in transepithelial electrical resistance; (b) alteration in the pattern of junctional complex proteins distribution as obseryed for E-cadherin, occludin and ZO-1; and (c) enlargement of the spaces between epithelial cells. These effects were dependent on (a) the degree of the parasite virulence isolate, (b) the iron concentration in the culture medium, and (c) the expression of adhesin proteins on the parasite surface.

  17. Intraepithelial lymphocytes express junctional molecules in murine small intestine

    International Nuclear Information System (INIS)

    Inagaki-Ohara, Kyoko; Sawaguchi, Akira; Suganuma, Tatsuo; Matsuzaki, Goro; Nawa, Yukifumi

    2005-01-01

    Intestinal intraepithelial lymphocytes (IEL) that reside at basolateral site regulate the proliferation and differentiation of epithelial cells (EC) for providing a first line of host defense in intestine. However, it remains unknown how IEL interact and communicate with EC. Here, we show that IEL express junctional molecules like EC. We identified mRNA expression of the junctional molecules in IEL such as zonula occludens (ZO)-1, occludin and junctional adhesion molecule (JAM) (tight junction), β-catenin and E-cadherin (adherens junction), and connexin26 (gap junction). IEL constitutively expressed occludin and E-cadherin at protein level, while other T cells in the thymus, spleen, liver, mesenteric lymph node, and Peyer's patches did not. γδ IEL showed higher level of these expressions than αβ IEL. The expression of occludin was augmented by anti-CD3 Ab stimulation. These results suggest the possibility of a novel role of IEL concerning epithelial barrier and communication between IEL and EC

  18. Breaking into the epithelial apical-junctional complex--news from pathogen hackers.

    Science.gov (United States)

    Vogelmann, Roger; Amieva, Manuel R; Falkow, Stanley; Nelson, W James

    2004-02-01

    The epithelial apical-junctional complex is a key regulator of cellular functions. In addition, it is an important target for microbial pathogens that manipulate the cell to survive, proliferate and sometimes persist within a host. Out of a myriad of potential molecular targets, some bacterial and viral pathogens have selected a subset of protein targets at the apical-junctional complex of epithelial cells. Studying how microbes use these targets also teaches us about the inherent physiological properties of host molecules in the context of normal junctional structure and function. Thus, we have learned that three recently uncovered components of the apical-junctional complex of the Ig superfamily--junctional adhesion molecule, Nectin and the coxsackievirus and adenovirus receptor--are important regulators of junction structure and function and represent critical targets of microbial virulence gene products.

  19. Breaking into the epithelial apical–junctional complex — news from pathogen hackers

    Science.gov (United States)

    Vogelmann, Roger; Amieva, Manuel R; Falkow, Stanley; Nelson, W James

    2012-01-01

    The epithelial apical–junctional complex is a key regulator of cellular functions. In addition, it is an important target for microbial pathogens that manipulate the cell to survive, proliferate and sometimes persist within a host. Out of a myriad of potential molecular targets, some bacterial and viral pathogens have selected a subset of protein targets at the apical–junctional complex of epithelial cells. Studying how microbes use these targets also teaches us about the inherent physiological properties of host molecules in the context of normal junctional structure and function. Thus, we have learned that three recently uncovered components of the apical–junctional complex of the Ig superfamily — junctional adhesion molecule, Nectin and the coxsackievirus and adenovirus receptor — are important regulators of junction structure and function and represent critical targets of microbial virulence gene products. PMID:15037310

  20. Vinculin but not alpha-actinin is a target of PKC phosphorylation during junctional assembly induced by calcium

    DEFF Research Database (Denmark)

    Perez-Moreno, M; Avila, A; Islas, S

    1998-01-01

    and alpha-actinin, two actin binding proteins of the adherent junctions. We found that during the junctional sealing induced by Ca2+, both proteins move towards the cell periphery and, while there is a significant increase in the phosphorylation of vinculin, alpha-actinin remains unchanged. The increased...

  1. Human zonulin, a potential modulator of intestinal tight junctions.

    Science.gov (United States)

    Wang, W; Uzzau, S; Goldblum, S E; Fasano, A

    2000-12-01

    Intercellular tight junctions are dynamic structures involved in vectorial transport of water and electrolytes across the intestinal epithelium. Zonula occludens toxin derived from Vibrio cholerae interacts with a specific intestinal epithelial surface receptor, with subsequent activation of a complex intracellular cascade of events that regulate tight junction permeability. We postulated that this toxin may mimic the effect of a functionally and immunologically related endogenous modulator of intestinal tight junctions. Affinity-purified anti-zonula occludens toxin antibodies and the Ussing chamber assay were used to screen for one or more mammalian zonula occludens toxin analogues in both fetal and adult human intestine. A novel protein, zonulin, was identified that induces tight junction disassembly in non-human primate intestinal epithelia mounted in Ussing chambers. Comparison of amino acids in the active zonula occludens toxin fragment and zonulin permitted the identification of the putative receptor binding domain within the N-terminal region of the two proteins. Zonulin likely plays a pivotal role in tight junction regulation during developmental, physiological, and pathological processes, including tissue morphogenesis, movement of fluid, macromolecules and leukocytes between the intestinal lumen and the interstitium, and inflammatory/autoimmune disorders.

  2. Intracellular trafficking pathways of Cx43 gap junction channels.

    Science.gov (United States)

    Epifantseva, Irina; Shaw, Robin M

    2018-01-01

    Gap Junction (GJ) channels, including the most common Connexin 43 (Cx43), have fundamental roles in excitable tissues by facilitating rapid transmission of action potentials between adjacent cells. For instance, synchronization during each heartbeat is regulated by these ion channels at the cardiomyocyte cell-cell border. Cx43 protein has a short half-life, and rapid synthesis and timely delivery of those proteins to particular subdomains are crucial for the cellular organization of gap junctions and maintenance of intracellular coupling. Impairment in gap junction trafficking contributes to dangerous complications in diseased hearts such as the arrhythmias of sudden cardiac death. Of recent interest are the protein-protein interactions with the Cx43 carboxy-terminus. These interactions have significant impact on the full length Cx43 lifecycle and also contribute to trafficking of Cx43 as well as possibly other functions. We are learning that many of the known non-canonical roles of Cx43 can be attributed to the recently identified six endogenous Cx43 truncated isoforms which are produced by internal translation. In general, alternative translation is a new leading edge for proteome expansion and therapeutic drug development. This review highlights recent mechanisms identified in the trafficking of gap junction channels, involvement of other proteins contributing to the delivery of channels to the cell-cell border, and understanding of possible roles of the newly discovered alternatively translated isoforms in Cx43 biology. This article is part of a Special Issue entitled: Gap Junction Proteins edited by Jean Claude Herve. Copyright © 2017 Elsevier B.V. All rights reserved.

  3. Silver nanoparticles increase connexin43-mediated gap junctional intercellular communication in HaCaT cells through activation of reactive oxygen species and mitogen-activated protein kinase signal pathway

    DEFF Research Database (Denmark)

    Qin, Yu; Han, Limin; Yang, Di

    2018-01-01

    Silver nanoparticles (AgNPs) are widely used in health and consumer products that routinely contact skin. However, the biological effects and possible mechanisms of AgNPs on skin remain unclear. Gap junctional intercellular communication (GJIC) plays a critical role in multicellular organisms to ...

  4. TJ-II wave forms analysis with wavelets and support vector machines

    International Nuclear Information System (INIS)

    Dormido-Canto, S.; Farias, G.; Dormido, R.; Vega, J.; Sanchez, J.; Santos, M.

    2004-01-01

    Since the fusion plasma experiment generates hundreds of signals, it is essential to have automatic mechanisms for searching similarities and retrieving of specific data in the wave form database. Wavelet transform (WT) is a transformation that allows one to map signals to spaces of lower dimensionality. Support vector machine (SVM) is a very effective method for general purpose pattern recognition. Given a set of input vectors which belong to two different classes, the SVM maps the inputs into a high-dimensional feature space through some nonlinear mapping, where an optimal separating hyperplane is constructed. In this work, the combined use of WT and SVM is proposed for searching and retrieving similar wave forms in the TJ-II database. In a first stage, plasma signals will be preprocessed by WT to reduce their dimensionality and to extract their main features. In the next stage, and using the smoothed signals produced by the WT, SVM will be applied to show up the efficiency of the proposed method to deal with the problem of sorting out thousands of fusion plasma signals.From observation of several experiments, our WT+SVM method is very viable, and the results seems promising. However, we have further work to do. We have to finish the development of a Matlab toolbox for WT+SVM processing and to include new relevant features in the SVM inputs to improve the technique. We have also to make a better preprocessing of the input signals and to study the performance of other generic and self custom kernels. To reach it, and since the preprocessing stages are very time consuming, we are going to study the viability of using DSPs, RPGAs or parallel programming techniques to reduce the execution time

  5. The visible intensified cameras for plasma imaging in the TJ-II stellarator

    International Nuclear Information System (INIS)

    Cal, E. de la; Carralero, D.; Pablos, J.L. de; Alonso, A.; Rios, L.; Garcia Sanchez, P.; Hidalgo, C.

    2011-01-01

    Visible cameras are widely used in fusion experiments for diagnosis and for machine safety issues. They are generally used to monitor the plasma emission, but are also sensible to surface Blackbody radiation and Bremsstrahlung. Fast or high speed cameras capable of operating in the 10 5 frames per second speed range are today commercially available and offer the opportunity to plasma fusion researchers of two-dimensional (2D) imaging of fast phenomena such as turbulence, ELMs, disruptions, dust, etc. The tracking of these fast phenomena requires short exposure times down to the μ s range and the light intensity can be often near the signal to noise ratio limit especially in low plasma emission regions such as the far SOL Additionally, when using interference filters to monitor, e.g. impurity line emission, the photon flux is strongly reduced and the emission cannot be imaged at high speed. Therefore, the use of image intensifiers that amplify the light intensity onto the camera sensor can be of great help. The present work describes the use of intensifiers in the visible fast cameras of TJ-II stellarator. We have achieved spectroscopic plasma imaging of filtered impurity atomic line emission at short exposure times down to the 10 μ s range depending on atomic line and concentration. Additionally, plasma movies at velocities of 2x10 5 frames per second near the camera operation limit can be recorded with exposure times well below 1 μ s with sufficient signal to noise ratio. Although an increasing degradation of the image quality appears when raising the light amplification, an effective gain of up to two orders of magnitude of the light intensity is feasible for many applications (copyright 2011 WILEY-VCH Verlag GmbH and Co. KGaA, Weinheim) (orig.)

  6. Dependence of the trapping regions with the radial electric field in TJ-II

    International Nuclear Information System (INIS)

    Guasp, J.; Liniers, M.

    1997-01-01

    The influence of the electric field upon the radial and angular distributions of trapped ions of energies between 0.1 and 1 keV in TJ-II is analysed. Near the magnetic axis, with moderate electric fields, the trapped fraction increases for negative potentials and decreases for positive ones. Nevertheless for high potentials this decreasing for positive sign can be reversed. Near the plasma periphery trapping is affected only for high potentials and always increases, independently of the potential sign. The sensibility to negative potentials is always higher than for the positive ones. These trapping changes are almost uniform in poloidal and toroidal angular profiles and affect mainly to marginally trapped or passing particles. Nevertheless for high potentials or energie the resonances modify this behaviour and inhibit the increasing of trapping in the outer side or the torus. For the locally trapped population fraction a steady decrease with the potential appears independently of the sign, except very near the magnetic axis or the plasma periphery. The global result of all these partial effects is the presence of a wide trapping minimum for moderate positive electric potential, potential that increases with the ion energy as well as a strong increase for high electric fields of either sign, more visible for negative potentials and high energies. For the locally trapped population a steady decrease with potential appears independently of the sign. All these effects can be explained by the combined action of the appearance and elimination of radial magnetic barriers and of the electric field resonances. (Author)

  7. Experimental dependence of ECR plasma breakdown on wave polarization in the TJ-II stellarator

    International Nuclear Information System (INIS)

    Cappa, A.; Castejon, F.; Tabares, F.; Fernandez, A.; Tafalla, D.; Cal, E. de la; Estrada, T.; Nagasaki, K.

    2005-01-01

    Recently, second harmonic ECRH plasma breakdown and its dependence on the initial conditions such as neutral gas pressure, injected power or beam polarization has been the subject of theoretical as well as experimental work. Although those studies have been carried out in the context of stellarators, they are particularly relevant in the case of large tokamaks, such as ITER, where conventional inductive breakdown is expected to be strongly improved if ECRH is used. A matter of interest is the dependence of breakdown time on wave polarization. In the existing theoretical models, wave polarization is not taken into account because it is assumed that the injected ECRH power is initially scrambled by the vessel walls and that the energy source for breakdown is only due to the non-linear wave-particle interaction between deeply trapped electrons and the averaged electric field. However, while this seems reasonable for the very beginning of the discharge, it may not be so as we progress towards breakdown. Actually, as experiments in Heliotron J have demonstrated, wave polarization must be taken into account in the description of the energy source. Breakdown experiments in TJ-II were performed in order to get a deep insight into this matter and part of the results of breakdown dependence on wave polarization were already discussed. In particular, it was demonstrated that the hypothesis about the energy source used in the models is failing long before full ionization is completed. But more information in relation with our understanding of the second harmonic ECRH breakdown, such as the toroidal and radial breakdown propagation, which is seen to be non-diffusive, can be extracted. Thus, the experiment results provide valuable data that can be used not only to improve the energy source but also to include spatial dimensions in the breakdown models. (author)

  8. The visible intensified cameras for plasma imaging in the TJ-II stellarator

    Energy Technology Data Exchange (ETDEWEB)

    Cal, E. de la; Carralero, D.; Pablos, J.L. de; Alonso, A.; Rios, L.; Garcia Sanchez, P.; Hidalgo, C. (Laboratorio Nacional de Fusion, Asociacion Euratom-Ciemat, Av. Complutense 22, E-28040 Madrid)

    2011-09-15

    Visible cameras are widely used in fusion experiments for diagnosis and for machine safety issues. They are generally used to monitor the plasma emission, but are also sensible to surface Blackbody radiation and Bremsstrahlung. Fast or high speed cameras capable of operating in the 10{sup 5} frames per second speed range are today commercially available and offer the opportunity to plasma fusion researchers of two-dimensional (2D) imaging of fast phenomena such as turbulence, ELMs, disruptions, dust, etc. The tracking of these fast phenomena requires short exposure times down to the {mu} s range and the light intensity can be often near the signal to noise ratio limit especially in low plasma emission regions such as the far SOL Additionally, when using interference filters to monitor, e.g. impurity line emission, the photon flux is strongly reduced and the emission cannot be imaged at high speed. Therefore, the use of image intensifiers that amplify the light intensity onto the camera sensor can be of great help. The present work describes the use of intensifiers in the visible fast cameras of TJ-II stellarator. We have achieved spectroscopic plasma imaging of filtered impurity atomic line emission at short exposure times down to the 10 {mu} s range depending on atomic line and concentration. Additionally, plasma movies at velocities of 2x10{sup 5} frames per second near the camera operation limit can be recorded with exposure times well below 1 {mu} s with sufficient signal to noise ratio. Although an increasing degradation of the image quality appears when raising the light amplification, an effective gain of up to two orders of magnitude of the light intensity is feasible for many applications (copyright 2011 WILEY-VCH Verlag GmbH and Co. KGaA, Weinheim) (orig.)

  9. Instabilities in thin tunnel junctions

    International Nuclear Information System (INIS)

    Konkin, M.K.; Adler, J.G.

    1978-01-01

    Tunnel junctions prepared for inelastic electron tunneling spectroscopy are often plagued by instabilities in the 0-500-meV range. This paper relates the bias at which the instability occurs to the barrier thickness

  10. The Control of Junction Flows

    National Research Council Canada - National Science Library

    Smith, Charles

    1997-01-01

    An experimental study of the effects of spatially-limited (i.e. localized) surface suction on unsteady laminar and turbulent junction flows was performed using hydrogen bubble flow visualization and Particle Image Velocimetry (PIV...

  11. Josephson junctions with ferromagnetic interlayer

    International Nuclear Information System (INIS)

    Wild, Georg Hermann

    2012-01-01

    We report on the fabrication of superconductor/insulator/ferromagnetic metal/superconductor (Nb/AlO x /Pd 0.82 Ni 0.18 /Nb) Josephson junctions (SIFS JJs) with high critical current densities, large normal resistance times area products, and high quality factors. For these junctions, a transition from 0- to π-coupling is observed for a thickness d F =6 nm of the ferromagnetic Pd 0.82 Ni 0.18 interlayer. The magnetic field dependence of the critical current of the junctions demonstrates good spatial homogeneity of the tunneling barrier and ferromagnetic interlayer. Magnetic characterization shows that the Pd 0.82 Ni 0.18 has an out-of-plane anisotropy and large saturation magnetization indicating negligible dead layers at the interfaces. A careful analysis of Fiske modes up to about 400 GHz provides valuable information on the junction quality factor and the relevant damping mechanisms. Whereas losses due to quasiparticle tunneling dominate at low frequencies, at high frequencies the damping is explained by the finite surface resistance of the junction electrodes. High quality factors of up to 30 around 200 GHz have been achieved. They allow to study the junction dynamics, in particular the switching probability from the zero-voltage into the voltage state with and without microwave irradiation. The experiments with microwave irradiation are well explained within semi-classical models and numerical simulations. In contrast, at mK temperature the switching dynamics without applied microwaves clearly shows secondary quantum effects. Here, we could observe for the first time macroscopic quantum tunneling in Josephson junctions with a ferromagnetic interlayer. This observation excludes fluctuations of the critical current as a consequence of an unstable magnetic domain structure of the ferromagnetic interlayer and affirms the suitability of SIFS Josephson junctions for quantum information processing.

  12. Josephson junctions with ferromagnetic interlayer

    Energy Technology Data Exchange (ETDEWEB)

    Wild, Georg Hermann

    2012-03-04

    We report on the fabrication of superconductor/insulator/ferromagnetic metal/superconductor (Nb/AlO{sub x}/Pd{sub 0.82}Ni{sub 0.18}/Nb) Josephson junctions (SIFS JJs) with high critical current densities, large normal resistance times area products, and high quality factors. For these junctions, a transition from 0- to {pi}-coupling is observed for a thickness d{sub F}=6 nm of the ferromagnetic Pd{sub 0.82}Ni{sub 0.18} interlayer. The magnetic field dependence of the critical current of the junctions demonstrates good spatial homogeneity of the tunneling barrier and ferromagnetic interlayer. Magnetic characterization shows that the Pd{sub 0.82}Ni{sub 0.18} has an out-of-plane anisotropy and large saturation magnetization indicating negligible dead layers at the interfaces. A careful analysis of Fiske modes up to about 400 GHz provides valuable information on the junction quality factor and the relevant damping mechanisms. Whereas losses due to quasiparticle tunneling dominate at low frequencies, at high frequencies the damping is explained by the finite surface resistance of the junction electrodes. High quality factors of up to 30 around 200 GHz have been achieved. They allow to study the junction dynamics, in particular the switching probability from the zero-voltage into the voltage state with and without microwave irradiation. The experiments with microwave irradiation are well explained within semi-classical models and numerical simulations. In contrast, at mK temperature the switching dynamics without applied microwaves clearly shows secondary quantum effects. Here, we could observe for the first time macroscopic quantum tunneling in Josephson junctions with a ferromagnetic interlayer. This observation excludes fluctuations of the critical current as a consequence of an unstable magnetic domain structure of the ferromagnetic interlayer and affirms the suitability of SIFS Josephson junctions for quantum information processing.

  13. First Toroidal Rotation Measurements of Protons and Impurities in the TJ-II Stellarator; Primeras Medidas de Rotacion Toroidal de Protones e Impurezas en el Stellarator TJ-II

    Energy Technology Data Exchange (ETDEWEB)

    Rapisarda, D.; Zurro, B.; Baciero, A.

    2006-07-01

    First absolute toroidal rotation measurements in the TJ-II stellarator, by using passive emission spectroscopy, are presented. The wavelength calibration is performed by using a spectral system which combines the spectra coming from the plasma and from a lamp in real time. Measurements have been made both for protons and some impurity ions (C4+, He+), in discharges created by electron cyclotron resonance heating, and in discharges with neutral beam injection heating. In addition, a description of the systems as well as the calibration procedures an data analysis is addressed. (Author) 10 refs.

  14. Electronic thermometry in tunable tunnel junction

    Science.gov (United States)

    Maksymovych, Petro

    2016-03-15

    A tunable tunnel junction thermometry circuit includes a variable width tunnel junction between a test object and a probe. The junction width is varied and a change in thermovoltage across the junction with respect to the change in distance across the junction is determined. Also, a change in biased current with respect to a change in distance across the junction is determined. A temperature gradient across the junction is determined based on a mathematical relationship between the temperature gradient, the change in thermovoltage with respect to distance and the change in biased current with respect to distance. Thermovoltage may be measured by nullifying a thermoelectric tunneling current with an applied voltage supply level. A piezoelectric actuator may modulate the probe, and thus the junction width, to vary thermovoltage and biased current across the junction. Lock-in amplifiers measure the derivatives of the thermovoltage and biased current modulated by varying junction width.

  15. Peltier cooling in molecular junctions

    Science.gov (United States)

    Cui, Longji; Miao, Ruijiao; Wang, Kun; Thompson, Dakotah; Zotti, Linda Angela; Cuevas, Juan Carlos; Meyhofer, Edgar; Reddy, Pramod

    2018-02-01

    The study of thermoelectricity in molecular junctions is of fundamental interest for the development of various technologies including cooling (refrigeration) and heat-to-electricity conversion1-4. Recent experimental progress in probing the thermopower (Seebeck effect) of molecular junctions5-9 has enabled studies of the relationship between thermoelectricity and molecular structure10,11. However, observations of Peltier cooling in molecular junctions—a critical step for establishing molecular-based refrigeration—have remained inaccessible. Here, we report direct experimental observations of Peltier cooling in molecular junctions. By integrating conducting-probe atomic force microscopy12,13 with custom-fabricated picowatt-resolution calorimetric microdevices, we created an experimental platform that enables the unified characterization of electrical, thermoelectric and energy dissipation characteristics of molecular junctions. Using this platform, we studied gold junctions with prototypical molecules (Au-biphenyl-4,4'-dithiol-Au, Au-terphenyl-4,4''-dithiol-Au and Au-4,4'-bipyridine-Au) and revealed the relationship between heating or cooling and charge transmission characteristics. Our experimental conclusions are supported by self-energy-corrected density functional theory calculations. We expect these advances to stimulate studies of both thermal and thermoelectric transport in molecular junctions where the possibility of extraordinarily efficient energy conversion has been theoretically predicted2-4,14.

  16. GAP junctional communication in brain secondary organizers.

    Science.gov (United States)

    Bosone, Camilla; Andreu, Abraham; Echevarria, Diego

    2016-06-01

    Gap junctions (GJs) are integral membrane proteins that enable the direct cytoplasmic exchange of ions and low molecular weight metabolites between adjacent cells. They are formed by the apposition of two connexons belonging to adjacent cells. Each connexon is formed by six proteins, named connexins (Cxs). Current evidence suggests that gap junctions play an important part in ensuring normal embryo development. Mutations in connexin genes have been linked to a variety of human diseases, although the precise role and the cell biological mechanisms of their action remain almost unknown. Among the big family of Cxs, several are expressed in nervous tissue but just a few are expressed in the anterior neural tube of vertebrates. Many efforts have been made to elucidate the molecular bases of Cxs cell biology and how they influence the morphogenetic signal activity produced by brain signaling centers. These centers, orchestrated by transcription factors and morphogenes determine the axial patterning of the mammalian brain during its specification and regionalization. The present review revisits the findings of GJ composed by Cx43 and Cx36 in neural tube patterning and discuss Cx43 putative enrollment in the control of Fgf8 signal activity coming from the well known secondary organizer, the isthmic organizer. © 2016 The Authors. Development, Growth & Differentiation published by John Wiley & Sons Australia, Ltd on behalf of Japanese Society of Developmental Biologists.

  17. Lamellar granule secretion starts before the establishment of tight junction barrier for paracellular tracers in mammalian epidermis.

    Directory of Open Access Journals (Sweden)

    Akemi Ishida-Yamamoto

    Full Text Available Defects in epidermal barrier function and/or vesicular transport underlie severe skin diseases including ichthyosis and atopic dermatitis. Tight junctions (TJs form a single layered network in simple epithelia. TJs are important for both barrier functions and vesicular transport. Epidermis is stratified epithelia and lamellar granules (LGs are secreted from the stratum granulosum (SG in a sequential manner. Previously, continuous TJs and paracellular permeability barriers were found in the second layer (SG2 of SG in mice, but their fate and correlation with LG secretion have been poorly understood. We studied epidermal TJ-related structures in humans and in mice and found occludin/ZO-1 immunoreactive multilayered networks spanning the first layer of SG (SG1 and SG2. Paracellular penetration tracer passed through some TJs in SG2, but not in SG1. LG secretion into the paracellular tracer positive spaces started below the level of TJs of SG1. Our study suggests that LG-secretion starts before the establishment of TJ barrier in the mammalian epidermis.

  18. Silver nanoparticles induce tight junction disruption and astrocyte neurotoxicity in a rat blood–brain barrier primary triple coculture model

    Directory of Open Access Journals (Sweden)

    Xu L

    2015-09-01

    tight junction (TJ protein ZO-1 was decreased. Discontinuous TJs were also observed between microvascular endothelial cells. After Ag-NPS exposure, severe mitochondrial shrinkage, vacuolations, endoplasmic reticulum expansion, and Ag-NPs were observed in astrocytes by TEM. Global gene expression analysis showed that three genes were upregulated and 20 genes were downregulated in astrocytes treated with Ag-NPS. Gene ontology (GO and Kyoto Encyclopedia of Genes and Genomes (KEGG pathway analysis showed that the 23 genes were associated with metabolic processes, biosynthetic processes, response to stimuli, cell death, the MAPK pathway, and so on. No GO term and KEGG pathways were changed in the released-ion or polystyrene-NP groups. Ag-NPS inhibited the antioxidant defense of the astrocytes by increasing thioredoxin interacting protein, which inhibits the Trx system, and decreasing Nr4a1 and Dusp1. Meanwhile, Ag-NPS induced inflammation and apoptosis through modulation of the MAPK pathway or B-cell lymphoma-2 expression or mTOR activity in astrocytes.Conclusion: These results draw our attention to the importance of Ag-NP-induced toxicity on the neurovascular unit and provide a better understanding of its toxicological mechanisms on astrocytes.Keywords: Ag nanoparticles, astrocytes, BBB model, global gene expression analysis, antioxidant defense

  19. Experimental evidence of significant temperature fluctuations in the plasma EDGE region of the TJ-I Tokamak

    Energy Technology Data Exchange (ETDEWEB)

    Hidalgo, C; Balbin, R; Pedrosa, M A; Garcia-Cortes, I; Ochando, M A

    1993-07-01

    Density and temperature fluctuations have been measured in the plasma bulk side of the velocity shear location of the TJ-I tokamak using a feast swept Langmuir probe technique. Evidence of substantial temperature fluctuations which are in phase close to opposition with the corresponding density fluctuations has been found. This result suggests the possible role of radiation in determining edge fluctuation levels and call into question the determination of the density and potential fluctuations from the Langmuir current-probe and floating potential fluctuations. (Author) 16 refs.

  20. Experimental evidence of significant temperature fluctuations in the plasma edge region of the TJ-I Tokamak

    International Nuclear Information System (INIS)

    Hidalgo, C.; Balbin, R.; Pedrosa, M.A.; Garcia-Cortes, I.; Ochando, M.A.

    1993-01-01

    Density and temperature fluctuations have been measured in the plasma bulk side of the velocity shear location of the TJ-I tokamak using a foast swept Langmuir probe technique. Evidence of sustantial temperature fluctuations which are in phase close to opposition with the corresponding density fluctuations has been found. This result suggests the possible role of radiation in determining edge fluctuation levels and call into question the determination of the density and potential fluctuations from the Langmuir current-probe and floating potential fluctuations. (Author)

  1. Experimental evidence of significant temperature fluctuations in the plasma EDGE region of the TJ-I Tokamak

    International Nuclear Information System (INIS)

    Hidalgo, C.; Balbin, R.; Pedrosa, M. A.; Garcia-Cortes, I.; Ochando, M. A.

    1993-01-01

    Density and temperature fluctuations have been measured in the plasma bulk side of the velocity shear location of the TJ-I tokamak using a feast swept Langmuir probe technique. Evidence of substantial temperature fluctuations which are in phase close to opposition with the corresponding density fluctuations has been found. This result suggests the possible role of radiation in determining edge fluctuation levels and call into question the determination of the density and potential fluctuations from the Langmuir current-probe and floating potential fluctuations. (Author) 16 refs

  2. Comparison of ATF and TJ-II stellarator equilibria as computed by the 3-D VMEC and PIES codes

    International Nuclear Information System (INIS)

    Johnson, J.L.; Monticello, D.A.; Reiman, A.H.; Hirshman, S.P.

    1992-01-01

    A comparison is made of results from the PIES code, which determines the equilibrium properties of three-dimensional toroidal configurations by direct integration along the magnetic field lines, with those from the VMEC code, which uses an energy minimization in a flux representation to determine the equilibrium configuration, for two devices: the ATF stellarator at Oak Ridge and the TJ-11 heliac which is being built in Madrid. The results obtained from the two codes are in good agreement, providing additional validation for the codes

  3. Chlorpromazine reduces the intercellular communication via gap junctions in mammalian cells

    International Nuclear Information System (INIS)

    Orellana, Juan A.; Palacios-Prado, Nicolas; Saez, Juan C.

    2006-01-01

    In the work presented herein, we evaluated the effect of chlorpromazine (CPZ) on gap junctions expressed by two mammalian cell types; Gn-11 cells (cell line derived from mouse LHRH neurons) and rat cortical astrocytes maintained in culture. We also attempted to elucidate possible mechanisms of action of CPZ effects on gap junctions. CPZ, in concentrations comparable with doses used to treat human diseases, was found to reduce the intercellular communication via gap junctions as evaluated with measurements of dye coupling (Lucifer yellow). In both cell types, maximal inhibition of functional gap junctions was reached within about 1 h of treatment with CPZ, an recovery was almost complete at about 5 h after CPZ wash out. In both cell types, CPZ treatment increased the phosphorylation state of connexin43 (Cx43), a gap junction protein subunit. Moreover, CPZ reduced the reactivity of Cx43 (immunofluorescence) at cell interfaces and concomitantly increased its reactivity in intracellular vesicles, suggesting an increased retrieval from and/or reduced insertion into the plasma membrane. CPZ also caused cellular retraction reducing cell-cell contacts in a reversible manner. The reduction in contact area might destabilize existing gap junctions and abrogate formation of new ones. Moreover, the CPZ-induced reduction in gap junctional communication may depend on the connexins (Cxs) forming the junctions. If Cx43 were the only connexin expressed, MAPK-dependent phosphorylation of this connexin would induce closure of gap junction channels

  4. Roles of gap junctions, connexins and pannexins in epilepsy

    Directory of Open Access Journals (Sweden)

    Shanthini eMylvaganam

    2014-05-01

    Full Text Available Enhanced gap junctional communication (GJC between neurons is considered a major factor underlying the neuronal synchrony driving seizure activity. In addition, the hippocampal sharp wave ripple complexes, associated with learning and seizures, are diminished by GJC blocking agents. Although gap junctional blocking drugs inhibit experimental seizures, they all have other nonspecific actions. Besides interneuronal GJC between dendrites, inter-axonal and inter-glial GJC is also considered important for seizure generation. Interestingly, in most studies of cerebral tissue from animal seizure models and from human patients with epilepsy, there is up-regulation of glial, but not neuronal gap junctional mRNA and protein. Significant changes in the expression and post-translational modification of the astrocytic connexin Cx43, and Panx1 were observed in an in vitro Co++ seizure model, further supporting a role for glia in seizure-genesis, although the reasons for this remain unclear. Further suggesting an involvement of astrocytic GJC in epilepsy, is the fact that the expression of astrocytic Cx mRNAs (Cxs 30 and 43 is several fold higher than that of neuronal Cx mRNAs (Cxs 36 and 45, and the number of glial cells outnumber neuronal cells in mammalian hippocampal and cortical tissue. Pannexin expression is also increased in both animal and human epileptic tissues. Specific Cx43 mimetic peptides, Gap 27 and SLS, inhibit the docking of astrocytic connexin Cx43 proteins from forming intercellular gap junctions, diminishing spontaneous seizures. Besides GJs, Cx membrane hemichannels in glia and Panx membrane channels in neurons and glia are also inhibited by gap junctional pharmacological blockers. Although there is no doubt that connexin-based gap junctions and hemichannels, and pannexin-based membrane channels are related to epilepsy, the specific details of how they are involved and how we can modulate their function for therapeutic purposes remain to

  5. Integration of autonomous systems for remote control of data acquisition and diagnostics in the TJ-II device

    International Nuclear Information System (INIS)

    Vega, J.; Mollinedo, A.; Lopez, A.; Pacios, L.; Dormido, S.

    1997-01-01

    The data acquisition system for TJ-II will consist of a central computer, containing the data base of the device, and a set of independent systems (personal computers, embedded ones, workstations, minicomputers, PLCs, and microprocessor systems among others), controlling data collection, and automated diagnostics. Each autonomous system can be used to isolate and manage specific problems in the most efficient manner. These problems are related to data acquisition, hard (μs endash ms) real time requirements, soft (ms endash s) real time requirements, remote control of diagnostics, etc. In the operation of TJ-II, the programming of systems will be carried out from the central computer. Coordination and synchronization will be performed by linking systems to local area networks. Several Ethernet segments and FDDI rings will be used for these purposes. Programmable logic controller devices (PLCs) used for diagnostic low level control will be linked among them through a fast serial link, the RS485 Profibus standard. One VME crate, running on the OS-9 real time operating system, will be assigned as a gateway, so as to connect the PLCs based systems with an Ethernet segment. copyright 1997 American Institute of Physics

  6. Effect of magnetic configuration on frequency of NBI-driven Alfvén modes in TJ-II

    Science.gov (United States)

    Melnikov, A. V.; Ochando, M.; Ascasibar, E.; Castejon, F.; Cappa, A.; Eliseev, L. G.; Hidalgo, C.; Krupnik, L. I.; Lopez-Fraguas, A.; Liniers, M.; Lysenko, S. E.; de Pablos, J. L.; Perfilov, S. V.; Sharapov, S. E.; Spong, D. A.; Jimenez, J. A.; Ufimtsev, M. V.; Breizman, B. N.; HIBP Group; the TJ-II Team

    2014-12-01

    Excitation of modes in the Alfvénic frequency range, 30 kHz values, 1.51advantage of the unique TJ-II capabilities, a dynamic magnetic configuration experiment with \\unicode{7548} (ρ , t) variation during discharges has shown strong effects on the mode frequency via both vacuum \\unicode{7548} changes and induced net plasma current. A drastic frequency increase from ˜50 to ˜250 kHz was observed for some modes when plasma current as low as ±2 kA was induced by small (10%) changes in the vertical field. A comprehensive set of diagnostics including a heavy ion beam probe, magnetic probes and a multi-chord bolometer made it possible to identify the spatial spread of the modes and deduce the internal amplitudes of their plasma density and magnetic field perturbations. A simple analytical model for fAE, based on the local Alfvén eigenmode (AE) dispersion relation, was proposed to characterize the observation. It was shown that all the observations, including vacuum iota and plasma current variations, may be fitted by the model, so the linear mode frequency dependence on \\unicode{7548} (plasma current) and one over square root density dependence present the major features of the NBI-induced AEs in TJ-II, and provide the framework for further experiment-to-theory comparison.

  7. Transport properties of molecular junctions

    CERN Document Server

    Zimbovskaya, Natalya A

    2013-01-01

    A comprehensive overview of the physical mechanisms that control electron transport and the characteristics of metal-molecule-metal (MMM) junctions is presented. As far as possible, methods and formalisms presented elsewhere to analyze electron transport through molecules are avoided. This title introduces basic concepts—a description of the electron transport through molecular junctions—and briefly describes relevant experimental methods. Theoretical methods commonly used to analyze the electron transport through molecules are presented. Various effects that manifest in the electron transport through MMMs, as well as the basics of density-functional theory and its applications to electronic structure calculations in molecules are presented. Nanoelectronic applications of molecular junctions and similar systems are discussed as well. Molecular electronics is a diverse and rapidly growing field. Transport Properties of Molecular Junctions presents an up-to-date survey of the field suitable for researchers ...

  8. NbN tunnel junctions

    International Nuclear Information System (INIS)

    Villegier, J.C.; Vieux-Rochaz, L.; Goniche, M.; Renard, P.; Vabre, M.

    1984-09-01

    All-niobium nitride Josephon junctions have been prepared successfully using a new processing called SNOP: Selective Niobium (nitride) Overlap Process. Such a process involves the ''trilayer'' deposition on the whole wafer before selective patterning of the electrodes by optically controlled dry reactive ion etching. Only two photomask levels are need to define an ''overlap'' or a ''cross-type'' junction with a good accuracy. The properties of the niobium nitride films deposited by DC-magnetron sputtering and the surface oxide growth are analysed. The most critical point to obtain high quality and high gap value junctions resides in the early stage of the NbN counterelectrode growth. Some possibilities to overcome such a handicap exist even if the fabrication needs substrate temperatures below 250 0 C

  9. Copper-induced tight junction mRNA expression changes, apoptosis and antioxidant responses via NF-κB, TOR and Nrf2 signaling molecules in the gills of fish: Preventive role of arginine

    International Nuclear Information System (INIS)

    Wang, Biao; Feng, Lin; Jiang, Wei-Dan; Wu, Pei; Kuang, Sheng-Yao; Jiang, Jun; Tang, Ling; Tang, Wu-Neng; Zhang, Yong-An; Liu, Yang

    2015-01-01

    Highlights: • Cu exposure induced oxidative stress via disruption of antioxidant system. • Cu exposure disrupted TJ mRNA expression through regulation of cytokines in fish. • Cu induced gill apoptosis partly via intrinsic pathway but not extrinsic pathway. • Cu exposure can regulate Nrf2, NF-κB and TOR signaling molecules in fish. • Arginine can effectively prevent Cu-induced fish gill damage. - Abstract: This study explored the possible preventive effects of dietary arginine on copper (Cu)-induced tight junction mRNA expression changes, apoptosis and antioxidant responses in the gills of young grass carp (Ctenopharyngodon idella). The results indicated that exposure to 0.7 mg/L (11.01 μmol/L) Cu for 96 h induced the production of reactive oxygen species (ROS), thereby increasing protein oxidation, lipid peroxidation and DNA damage in the gills of fish. However, these oxidative effects were prevented by arginine supplementation. Arginine also prevented the toxic effects of Cu on the activities of copper/zinc superoxide dismutase (SOD1), glutathione-S-transferase (GST), glutathione peroxidase (GPx), glutathione reductase (GR) and the glutathione (GSH) content (P < 0.05). However, Cu induced an adaptive increase in the activity of catalase (CAT), and arginine supplementation further increased CAT activity (P < 0.05). Moreover, Cu induced increases in the relative mRNA expressions of SOD1, CAT, GPx, GST, caspase-3, caspase-9, NF-E2-related factor 2 (Nrf2), Kelch-like-ECH-associated protein 1a (Keap1a), tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), interleukin-8 (IL-8), transforming growth factor-β (TGF-β) and nuclear transcription factor-κB p65 (NF-κB p65) in the gills of grass carp (P < 0.05). In contrast, the relative mRNA expression levels of occludin, zonula occludens-1 (ZO-1), claudin b, claudin 3, claudin 12, target of rapamycin (TOR) and inhibitor factor κBα (IκBα) in the gills were decreased by Cu (P < 0.05). However, pre

  10. Copper-induced tight junction mRNA expression changes, apoptosis and antioxidant responses via NF-κB, TOR and Nrf2 signaling molecules in the gills of fish: Preventive role of arginine

    Energy Technology Data Exchange (ETDEWEB)

    Wang, Biao [Animal Nutrition Institute, Sichuan Agricultural University, Chengdu 611130, Sichuan (China); Feng, Lin; Jiang, Wei-Dan [Animal Nutrition Institute, Sichuan Agricultural University, Chengdu 611130, Sichuan (China); Fish Nutrition and Safety Production University Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, Sichuan (China); Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Sichuan Agricultural University, Chengdu 611130, Sichuan (China); Wu, Pei [Animal Nutrition Institute, Sichuan Agricultural University, Chengdu 611130, Sichuan (China); Kuang, Sheng-Yao [Animal Nutrition Institute, Sichuan Academy of Animal Science, Chengdu, 610066, Sichuan (China); Jiang, Jun [Animal Nutrition Institute, Sichuan Agricultural University, Chengdu 611130, Sichuan (China); Fish Nutrition and Safety Production University Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, Sichuan (China); Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Sichuan Agricultural University, Chengdu 611130, Sichuan (China); Tang, Ling; Tang, Wu-Neng [Animal Nutrition Institute, Sichuan Academy of Animal Science, Chengdu, 610066, Sichuan (China); Zhang, Yong-An [Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072 (China); Liu, Yang, E-mail: kyckgk@hotmail.com [Animal Nutrition Institute, Sichuan Agricultural University, Chengdu 611130, Sichuan (China); Fish Nutrition and Safety Production University Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, Sichuan (China); Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Sichuan Agricultural University, Chengdu 611130, Sichuan (China); and others

    2015-01-15

    Highlights: • Cu exposure induced oxidative stress via disruption of antioxidant system. • Cu exposure disrupted TJ mRNA expression through regulation of cytokines in fish. • Cu induced gill apoptosis partly via intrinsic pathway but not extrinsic pathway. • Cu exposure can regulate Nrf2, NF-κB and TOR signaling molecules in fish. • Arginine can effectively prevent Cu-induced fish gill damage. - Abstract: This study explored the possible preventive effects of dietary arginine on copper (Cu)-induced tight junction mRNA expression changes, apoptosis and antioxidant responses in the gills of young grass carp (Ctenopharyngodon idella). The results indicated that exposure to 0.7 mg/L (11.01 μmol/L) Cu for 96 h induced the production of reactive oxygen species (ROS), thereby increasing protein oxidation, lipid peroxidation and DNA damage in the gills of fish. However, these oxidative effects were prevented by arginine supplementation. Arginine also prevented the toxic effects of Cu on the activities of copper/zinc superoxide dismutase (SOD1), glutathione-S-transferase (GST), glutathione peroxidase (GPx), glutathione reductase (GR) and the glutathione (GSH) content (P < 0.05). However, Cu induced an adaptive increase in the activity of catalase (CAT), and arginine supplementation further increased CAT activity (P < 0.05). Moreover, Cu induced increases in the relative mRNA expressions of SOD1, CAT, GPx, GST, caspase-3, caspase-9, NF-E2-related factor 2 (Nrf2), Kelch-like-ECH-associated protein 1a (Keap1a), tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), interleukin-8 (IL-8), transforming growth factor-β (TGF-β) and nuclear transcription factor-κB p65 (NF-κB p65) in the gills of grass carp (P < 0.05). In contrast, the relative mRNA expression levels of occludin, zonula occludens-1 (ZO-1), claudin b, claudin 3, claudin 12, target of rapamycin (TOR) and inhibitor factor κBα (IκBα) in the gills were decreased by Cu (P < 0.05). However, pre

  11. Gravitation at the Josephson Junction

    Directory of Open Access Journals (Sweden)

    Victor Atanasov

    2018-01-01

    Full Text Available A geometric potential from the kinetic term of a constrained to a curved hyperplane of space-time quantum superconducting condensate is derived. An energy conservation relation involving the geometric field at every material point in the superconductor is demonstrated. At a Josephson junction the energy conservation relation implies the possibility of transforming electric energy into geometric field energy, that is, curvature of space-time. Experimental procedures to verify that the Josephson junction can act as a voltage-to-curvature converter are discussed.

  12. Oral and Fecal Campylobacter concisus Strains induce Barrier dysfunction by Apoptosis in HT-29/B6 Intestinal Epithelial Cells

    DEFF Research Database (Denmark)

    Nielsen, Hans Linde; Nielsen, Henrik Ib; Ejlertsen, Tove

    in Ussing chambers. Tight junction (TJ) protein expression was determined by Western blotting, and subcellular TJ distribution was analyzed by confocal laser-scanning microscopy. Apoptosis induction was examined by TUNEL-staining and Western blot of caspase-3 activation. All strains invaded confluent HT-29...

  13. Electronic noise of superconducting tunnel junction detectors

    International Nuclear Information System (INIS)

    Jochum, J.; Kraus, H.; Gutsche, M.; Kemmather, B.; Feilitzsch, F. v.; Moessbauer, R.L.

    1994-01-01

    The optimal signal to noise ratio for detectors based on superconducting tunnel junctions is calculated and compared for the cases of a detector consisting of one single tunnel junction, as well as of series and of parallel connections of such tunnel junctions. The influence of 1 / f noise and its dependence on the dynamical resistance of tunnel junctions is discussed quantitatively. A single tunnel junction yields the minimum equivalent noise charge. Such a tunnel junction exhibits the best signal to noise ratio if the signal charge is independent of detector size. In case, signal charge increases with detector size, a parallel or a series connection of tunnel junctions would provide the optimum signal to noise ratio. The equivalent noise charge and the respective signal to noise ratio are deduced as functions of tunnel junction parameters such as tunneling time, quasiparticle lifetime, etc. (orig.)

  14. Current noise in tunnel junctions

    Energy Technology Data Exchange (ETDEWEB)

    Frey, Moritz; Grabert, Hermann [Physikalisches Institut, Universitaet Freiburg, Hermann-Herder-Strasse 3, 79104, Freiburg (Germany)

    2017-06-15

    We study current fluctuations in tunnel junctions driven by a voltage source. The voltage is applied to the tunneling element via an impedance providing an electromagnetic environment of the junction. We use circuit theory to relate the fluctuations of the current flowing in the leads of the junction with the voltage fluctuations generated by the environmental impedance and the fluctuations of the tunneling current. The spectrum of current fluctuations is found to consist of three parts: a term arising from the environmental Johnson-Nyquist noise, a term due to the shot noise of the tunneling current and a third term describing the cross-correlation between these two noise sources. Our phenomenological theory reproduces previous results based on the Hamiltonian model for the dynamical Coulomb blockade and provides a simple understanding of the current fluctuation spectrum in terms of circuit theory and properties of the average current. Specific results are given for a tunnel junction driven through a resonator. (copyright 2016 WILEY-VCH Verlag GmbH and Co. KGaA, Weinheim)

  15. Impacts of the Shine Through neutrals on the Vacuum Vessel of TJ-II during NBI; Impactos de los Neutros de Shine Through en la Camara de Vacio del TJ-II durante NBI

    Energy Technology Data Exchange (ETDEWEB)

    Guasp, J.; Liniers, M.

    1995-07-01

    A numerical analysis of the impact patterns on the Vacuum Vessel produced by Shine Through neutrals during the tangential balanced NBI in TJ-II Helical Axis Stellarator has been done. The results show two main concentrations. The first one the circular part of the Hard Core, near the zone of closest approach of the beam. The second one, the most important, around the beam exit, on the border between the plate of the HC cover and the sector wall. As expected, the Shine Through loads decrease strongly with plasma density, predominating at low density at NBI start, decreasing quickly when density increases and increasing slightly with the beam energy. No overlapping with lost fast ions impacts is observed, that, in addition, show an opposite behaviour with density. (Author) 3 refs.

  16. Remote Control System of the TJ-II Microwave Transmission Lines Mirrors; Sistema de Control Remoto de los Espejos de las Lineas de Transmision de Microondas del TJ-II

    Energy Technology Data Exchange (ETDEWEB)

    Lopez Sanchez, A.; Fernandez, A.; Cappa, A.; Gama, J. de la; Olivares, J.; Garcia, R.; Chamorro, M.

    2007-09-27

    The ECRH system of the TJ-II stellarator has two gyrotrons, which deliver a maximum power of 300 kW each at a frequency of 53.2 GHz. Another 28 GHz gyrotron will be used to heat the plasma by electron Bernstein waves (EBWH). The microwave power is transmitted from the gyrotrons to the vacuum chamber by two quasi-optical transmission lines for ECRH and a corrugated waveguide for EBWH. All transmission lines have an internal movable mirror inside the vacuum chamber to focus the beam and to be able to change the launching angle. The control of the beam polarization is very important and the lines have two corrugated mirrors, which actuate as polarizers. In this report the control system of the position of these three internal mirrors and the polarizers of the EBWH transmission line is described. (Author) 20 refs.

  17. Impacts of lost fast ions on the TJ-II Vacuum Vessel during NBI; Impactos de los iones rapidos en la Camara de Vacio del TJ-II durante NBI

    Energy Technology Data Exchange (ETDEWEB)

    Guasp, J

    1995-07-01

    The possible deposition patterns, on the Vacuum Vessel, of lost fast ions during the balanced tangential NBI in TJ-II helical axis Stellarator are analysed theoretically, establishing the relation between those impact points, the plasma exit and birth positions and the magnetic configuration characteristics. It is shown that direct losses are the most important, mainly those produced by the beam injected with the same direction that the magnetic field, increasing with beam energy and plasma density but with impacts remaining fixed on well defined zones, a periodically distributed along the Hard Core cover plates, producing high loads at high densities. The remaining losses, except for the shine through ones that predominate at low density, are periodically distributed, with smooth maxima and produce very low loads. No overlapping between the different kind of losses or beams is observed. (Author) 6 refs.

  18. Control of the Superconducting Magnets current Power Supplies of the TJ-II Gyrotrons; Control de las Fuentes de Corriente de las Bobinas Superconductoras de los Girotrones del TJ-II

    Energy Technology Data Exchange (ETDEWEB)

    Ros, A; Fernandez, A; Tolkachev, A; Catalan, G

    2006-07-01

    The TJ-II ECRH heating system consists of two gyrotrons, which can deliver a maximum power of 300 kW at a frequency of 53.2 GHz. Another 28 GHz gyrotron is going to be used in the Bernstein waves heating system. In order to get the required frequency, the gyrotrons need and homogeneous magnetic field of several tesla, which is generated by a superconducting coil field by a current source. This document describes the current source control as well as the high precision ammeters control. These ammeters measure the current in the superconducting coils. The user interface and the programming of the control system are described. The communication between devices is also explained. (author) 9 Refs.

  19. Stability of large-area molecular junctions

    NARCIS (Netherlands)

    Akkerman, Hylke B.; Kronemeijer, Auke J.; Harkema, Jan; van Hal, Paul A.; Smits, Edsger C. P.; de Leeuw, Dago M.; Blom, Paul W. M.

    The stability of molecular junctions is crucial for any application of molecular electronics. Degradation of molecular junctions when exposed to ambient conditions is regularly observed. In this report the stability of large-area molecular junctions under ambient conditions for more than two years

  20. Dynamics of pi-junction interferometer circuits

    DEFF Research Database (Denmark)

    Kornkev, V.K.; Mozhaev, P.B.; Borisenko, I.V.

    2002-01-01

    The pi-junction superconducting circuit dynamics was studied by means of numerical simulation technique. Parallel arrays consisting of Josephson junctions of both 0- and pi-type were studied as a model of high-T-c grain-boundary Josephson junction. The array dynamics and the critical current depe...

  1. Upgrade of the automatic analysis system in the TJ-II Thomson Scattering diagnostic: New image recognition classifier and fault condition detection

    NARCIS (Netherlands)

    Makili, L.; Vega, J.; Dormido-Canto, S.; Pastor, I.; Pereira, A.; Farias, G.; Portas, A.; Perez-Risco, D.; Rodriguez-Fernandez, M. C.; Busch, P.

    2010-01-01

    An automatic image classification system based on support vector machines (SVM) has been in operation for years in the TJ-II Thomson Scattering diagnostic. It recognizes five different types of images: CCD camera background, measurement of stray light without plasma or in a collapsed discharge,

  2. Hyperglycaemia and diabetes impair gap junctional communication among astrocytes.

    Science.gov (United States)

    Gandhi, Gautam K; Ball, Kelly K; Cruz, Nancy F; Dienel, Gerald A

    2010-03-15

    Sensory and cognitive impairments have been documented in diabetic humans and animals, but the pathophysiology of diabetes in the central nervous system is poorly understood. Because a high glucose level disrupts gap junctional communication in various cell types and astrocytes are extensively coupled by gap junctions to form large syncytia, the influence of experimental diabetes on gap junction channel-mediated dye transfer was assessed in astrocytes in tissue culture and in brain slices from diabetic rats. Astrocytes grown in 15-25 mmol/l glucose had a slow-onset, poorly reversible decrement in gap junctional communication compared with those grown in 5.5 mmol/l glucose. Astrocytes in brain slices from adult STZ (streptozotocin)-treated rats at 20-24 weeks after the onset of diabetes also exhibited reduced dye transfer. In cultured astrocytes grown in high glucose, increased oxidative stress preceded the decrement in dye transfer by several days, and gap junctional impairment was prevented, but not rescued, after its manifestation by compounds that can block or reduce oxidative stress. In sharp contrast with these findings, chaperone molecules known to facilitate protein folding could prevent and rescue gap junctional impairment, even in the presence of elevated glucose level and oxidative stress. Immunostaining of Cx (connexin) 43 and 30, but not Cx26, was altered by growth in high glucose. Disruption of astrocytic trafficking of metabolites and signalling molecules may alter interactions among astrocytes, neurons and endothelial cells and contribute to changes in brain function in diabetes. Involvement of the microvasculature may contribute to diabetic complications in the brain, the cardiovascular system and other organs.

  3. 7-Ketocholesterol modulates intercellular communication through gap-junction in bovine lens epithelial cells

    Directory of Open Access Journals (Sweden)

    Pereira Paulo

    2004-06-01

    Full Text Available Abstract Background Connexin43 (Cx43 is an integral membrane protein that forms intercellular channels called gap junctions. Intercellular communication in the eye lens relies on an extensive network of gap junctions essential for the maintenance of lens transparency. The association of Cx43 with cholesterol enriched lipid raft domains was recently demonstrated. The objective of this study is to assess if products of cholesterol oxidation (oxysterols affect gap junction intercellular communication (GJIC. Results Primary cultures of lens epithelial cells (LEC were incubated with 7-ketocholesterol (7-Keto, 25-hydroxycholesterol (25-OH or cholesterol and the subcellular distribution of Cx43 was evaluated by immunofluorescence confocal microscopy. The levels of Cx43 present in gap junction plaques were assessed by its insolubility in Triton X-100 and quantified by western blotting. The stability of Cx43 at the plasma membrane following incubation with oxysterols was evaluated by biotinylation of cell surface proteins. Gap junction intercellular communication was evaluated by transfer of the dye Lucifer yellow. The results obtained showed that 7-keto induces an accumulation of Cx43 at the plasma membrane and an increase in intercellular communication through gap junction. However, incubation with cholesterol or 25-OH did not lead to significant alterations on subcellular distribution of Cx43 nor in intercellular communication. Data further suggests that increased intercellular communication results from increased stability of Cx43 at the plasma membrane, presumably forming functional gap-junctions, as suggested by decreased solubility of Cx43 in 1% Triton X-100. The increased stability of Cx43 at the plasma membrane seems to be specific and not related to disruption of endocytic pathway, as demonstrated by dextran uptake. Conclusions Results demonstrate, for the first time, that 7-keto induces an increase in gap junction intercellular communication

  4. Role of rational surfaces on fluctuations and transport in the plasma edge of the TJ-II stellarator

    International Nuclear Information System (INIS)

    Pedrosa, M.A.; Hidalgo, C.; Lopez-Fraguas, A.

    2000-01-01

    It has been shown that transport barriers in toroidal magnetically confined plasmas tend to be linked to regions of unique magnetic topology such as the location of a minimum in the safety factor, rational surfaces or the boundary between closed and open flux surfaces. In the absence of E x B sheared flows, fluctuations are expected to show maximum amplitude near rational surfaces, and plasma confinement might tend to deteriorate. On the other hand, if the generation of E x B sheared flows were linked to low order rational surfaces, these would be beneficial to confinement. Experimental evidence of E x B sheared flows linked to rational surfaces has been obtained in the plasma edge region of the TJ-II stellarator. (author)

  5. Influence of low-order rational magnetic surfaces on heat transport in TJ-II heliac ECRH plasmas

    International Nuclear Information System (INIS)

    Castejon, F.; Lopez-Bruna, D.; Estrada, T.; Ascasibar, E.; Zurro, B.; Baciero, A.

    2004-01-01

    We study the effect of low-order rational surfaces on electron heat transport in plasmas confined in the TJ-II stellarator (Alejaldre et al 1990 Fusion Technol. 17 131) and heated by electron cyclotron waves. Enhancement of core electron heat confinement is observed when the rational surface is placed in the vicinity of the power deposition zone, either by performing a magnetic configuration scan or by inducing Ohmic current in a single discharge. The key to improving heat confinement seems to be a locally strong positive radial electric field, which is made possible by a synergistic effect between enhanced electron heat fluxes through radial positions around low-order rationals and pump out mechanisms in the heat deposition zone. (author)

  6. Ferromagnetic Josephson Junctions for Cryogenic Memory

    Science.gov (United States)

    Niedzielski, Bethany M.; Gingrich, Eric C.; Khasawneh, Mazin A.; Loloee, Reza; Pratt, William P., Jr.; Birge, Norman O.

    2015-03-01

    Josephson junctions containing ferromagnetic materials are of interest for both scientific and technological purposes. In principle, either the amplitude of the critical current or superconducting phase shift across the junction can be controlled by the relative magnetization directions of the ferromagnetic layers in the junction. Our approach concentrates on phase control utilizing two junctions in a SQUID geometry. We will report on efforts to control the phase of junctions carrying either spin-singlet or spin-triplet supercurrent for cryogenic memory applications. Supported by Northorp Grumman Corporation and by IARPA under SPAWAR Contract N66001-12-C-2017.

  7. Method of manufacturing Josephson junction integrated circuits

    International Nuclear Information System (INIS)

    Jillie, D.W. Jr.; Smith, L.N.

    1985-01-01

    Josephson junction integrated circuits of the current injection type and magnetically controlled type utilize a superconductive layer that forms both Josephson junction electrode for the Josephson junction devices on the integrated circuit as well as a ground plane for the integrated circuit. Large area Josephson junctions are utilized for effecting contact to lower superconductive layers and islands are formed in superconductive layers to provide isolation between the groudplane function and the Josephson junction electrode function as well as to effect crossovers. A superconductor-barrier-superconductor trilayer patterned by local anodization is also utilized with additional layers formed thereover. Methods of manufacturing the embodiments of the invention are disclosed

  8. DHT deficiency perturbs the integrity of the rat seminiferous epithelium by disrupting tight and adherens junctions.

    Science.gov (United States)

    Kolasa, Agnieszka; Marchlewicz, Mariola; Wenda-Różewicka, Lidia; Wiszniewska, Barbara

    2011-01-01

    In rats with a DHT deficiency induced by finasteride, morphological changes in the seminiferous epithelium were observed. The structural alterations were manifested by the premature germ cells sloughing into the lumen of seminiferous tubules. The etiology of this disorder could be connected with intercellular junctions disintegration. We showed in the immunohistochemical study the changes in expression of some proteins building tight and adherens junctions. The depression of N-cadherin, β-catenin and occludin immunoexpressions could be the reason for the release of immature germ cells from the seminiferous epithelium. However, the observed increase of the immunohistochemical reaction intensity of vinculin, one of the cadherin/catenin complex regulators, could be insufficient to maintain the proper function of adherens junctions. The hormonal imbalance appears to influence the pattern of expression of junctional proteins in the seminiferous epithelium. It could lead to untimely germ cells sloughing, and ultimately could impair fertility.

  9. ATP- and gap junction-dependent intercellular calcium signaling in osteoblastic cells

    DEFF Research Database (Denmark)

    Jorgensen, N R; Geist, S T; Civitelli, R

    1997-01-01

    mechanically induced calcium waves in two rat osteosarcoma cell lines that differ in the gap junction proteins they express, in their ability to pass microinjected dye from cell to cell, and in their expression of P2Y2 (P2U) purinergic receptors. ROS 17/2.8 cells, which express the gap junction protein......Many cells coordinate their activities by transmitting rises in intracellular calcium from cell to cell. In nonexcitable cells, there are currently two models for intercellular calcium wave propagation, both of which involve release of inositol trisphosphate (IP3)- sensitive intracellular calcium...... stores. In one model, IP3 traverses gap junctions and initiates the release of intracellular calcium stores in neighboring cells. Alternatively, calcium waves may be mediated not by gap junctional communication, but rather by autocrine activity of secreted ATP on P2 purinergic receptors. We studied...

  10. Effects of the radial electric field resonances on the particle orbits and loss cones in TJ-II

    International Nuclear Information System (INIS)

    Guasp, J.; Liniers, M.

    1997-07-01

    The effects of the radial electric field resonances on the trapping and confinement of low and intermediate energy ions (0.1-1 keV) for the Reference configuration of TJ-II have been analysed. In TJ-II these resonances appear for electric potentials that grow with pitch and with the square root of the initial kinetic energy and are placed inside strips whose width increases with the initial radius and with the absolute value of initial pitch. The 0-Resonance is the most important one for particle trapping, it appears for high electric potential (between 1000 and 3000 V for 0.5 keV ions) with the same sign than pitch, inside very wide strips (several thousands of V). Along these band periphery, for potential intensities below the central resonant values, there exists a very strong increase of particle trapping. Instead, around the resonance center, the trapping is inhibited and a very strong increase of the passing particle population appears. This increase is higher for the torus external side (Theta approximately 0 degree centigree) and for small initial radius. For peripherical particles wide loss strips appear along the border of the resonant band corresponding to more positive potential. The 2-Resonance has small effect on trapping but affects strongly to the of peripherical passing particles. It appears for moderate electric potential (between 400 and 1000 V for 0.5 keV ions) with sign opposite than pitch and inside narrow bands than the 0-Resonance. In this case a loss of peripherical passing particles appears, placed also along the more positive potential band side. The other Resonances (except the -4) have much less effect on particle trapping and confinement. All these phenomena have been explained by the action of magnetic barriers and different mechanisms for particle orbit modification. (Author) 8 refs

  11. Heavy ion beam probe investigations of plasma potential in ECRH and NBI in the TJ-II stellarator

    International Nuclear Information System (INIS)

    Melnikov, A.V.; Eliseev, L.; Perfilov, S.V.; Chmyga, A.A.; Dreval, N.; Khrebtov, S.M.; Komarov, A.D.; Kozachok, A.S.; Krupnik, L.; Alonso, A.; Pablos, J.L. de; Cappa, A.; Fernandez, A.; Fuentes, C.; Hidalgo, C.; Liniers, M.; Pedrosa, M.A.

    2005-01-01

    Direct measurements of electric potential and its fluctuations are of a primary importance in magnetic confinement systems. The Heavy Ion Beam Probe (HIBP) diagnostic is used in TJ-2 stellarator to study directly plasma electric potential profiles with spatial (up to 1cm) and temporal (up to 10 ∝s) resolution. The singly charged heavy ions Cs + with energies up to 125 keV are used to probe the plasma column from the edge to the core. Both ECRH and NBI heated plasmas (P ECRH = 200 - 400kW, P NBI = 400kW, E NBI = 28 kV) were studied. The significant improvement in the HIBP beam control system and the acquisition electronics leads us to increase the possibilities of the diagnostic. The most crucial one is the extension of the signal dynamic range, which allows us to have the reliable profiles from the plasma center to the plasma edge both in the high and low field side regions. Low density ECRH (n = 0.5-1.1.10 13 cm -3 ) plasmas in TJ-2 are characterised by core positive plasma potential of order of 500 - 1000 V and positive electric fields up to 50 V/cm. Edge radial electric fields remain positive at low densities and became negative at the threshold density that depends of plasma configuration. NBI plasmas are characterized by negative electric potential in the full plasma column and negative radial electric fields (in the range of 10 - 40 V/cm). The density rise during the NBI phase is accompanied by the decay of core plasma potential. When density is getting the level of n ∼ 2.0.10 13 cm -3 , the potential stops its evolution and remains constant. The evolution of plasma potential near density limit is under investigation. These observations, reported in different magnetic configurations, show the clear link between plasma potential and plasma density. (author)

  12. Molecular series-tunneling junctions.

    Science.gov (United States)

    Liao, Kung-Ching; Hsu, Liang-Yan; Bowers, Carleen M; Rabitz, Herschel; Whitesides, George M

    2015-05-13

    Charge transport through junctions consisting of insulating molecular units is a quantum phenomenon that cannot be described adequately by classical circuit laws. This paper explores tunneling current densities in self-assembled monolayer (SAM)-based junctions with the structure Ag(TS)/O2C-R1-R2-H//Ga2O3/EGaIn, where Ag(TS) is template-stripped silver and EGaIn is the eutectic alloy of gallium and indium; R1 and R2 refer to two classes of insulating molecular units-(CH2)n and (C6H4)m-that are connected in series and have different tunneling decay constants in the Simmons equation. These junctions can be analyzed as a form of series-tunneling junctions based on the observation that permuting the order of R1 and R2 in the junction does not alter the overall rate of charge transport. By using the Ag/O2C interface, this system decouples the highest occupied molecular orbital (HOMO, which is localized on the carboxylate group) from strong interactions with the R1 and R2 units. The differences in rates of tunneling are thus determined by the electronic structure of the groups R1 and R2; these differences are not influenced by the order of R1 and R2 in the SAM. In an electrical potential model that rationalizes this observation, R1 and R2 contribute independently to the height of the barrier. This model explicitly assumes that contributions to rates of tunneling from the Ag(TS)/O2C and H//Ga2O3 interfaces are constant across the series examined. The current density of these series-tunneling junctions can be described by J(V) = J0(V) exp(-β1d1 - β2d2), where J(V) is the current density (A/cm(2)) at applied voltage V and βi and di are the parameters describing the attenuation of the tunneling current through a rectangular tunneling barrier, with width d and a height related to the attenuation factor β.

  13. Flexible 2D layered material junctions

    Science.gov (United States)

    Balabai, R.; Solomenko, A.

    2018-03-01

    Within the framework of the methods of the electron density functional and the ab initio pseudopotential, we have obtained the valence electron density spatial distribution, the densities of electron states, the widths of band gaps, the charges on combined regions, and the Coulomb potentials for graphene-based flexible 2D layered junctions, using author program complex. It is determined that the bending of the 2D layered junctions on the angle α leads to changes in the electronic properties of these junctions. In the graphene/graphane junction, there is clear charge redistribution with different signs in the regions of junctions. The presence in the heterojunctions of charge regions with different signs leads to the formation of potential barriers. The greatest potential jump is in the graphene/fluorographene junction. The greatest value of the band gap width is in the graphene/graphane junction.

  14. Specificity of binding to four-way junctions in DNA by bacteriophage T7 endonuclease I.

    OpenAIRE

    Parsons, C A; West, S C

    1990-01-01

    T7 endonuclease I binds specifically to four-way junctions in duplex DNA and promotes their resolution into linear duplexes. Under conditions in which the nuclease activity is blocked by the absence of divalent cations, the enzyme forms a distinct protein-DNA complex with the junction, as detected by gel retardation and filter binding assays. The formation of this complex is structure-specific and contrasts with the short-lived binding complexes formed on linear duplex DNA. The binding comple...

  15. [Gap junctions: A new therapeutic target in major depressive disorder?].

    Science.gov (United States)

    Sarrouilhe, D; Dejean, C

    2015-11-01

    Major depressive disorder is a multifactorial chronic and debilitating mood disease with high lifetime prevalence and is associated with excess mortality, especially from cardiovascular diseases and through suicide. The treatments of this disease with tricyclic antidepressants and monoamine oxidase inhibitors are poorly tolerated and those that selectively target serotonin and norepinephrine re-uptake are not effective in all patients, showing the need to find new therapeutic targets. Post-mortem studies of brains from patients with major depressive disorders described a reduced expression of the gap junction-forming membrane proteins connexin 30 and connexin 43 in the prefrontal cortex and the locus coeruleus. The use of chronic unpredictable stress, a rodent model of depression, suggests that astrocytic gap junction dysfunction contributes to the pathophysiology of major depressive disorder. Chronic treatments of rats with fluoxetine and of rat cultured cortical astrocytes with amitriptyline support the hypothesis that the upregulation of gap junctional intercellular communication between brain astrocytes could be a novel mechanism for the therapeutic effect of antidepressants. In conclusion, astrocytic gap junctions are emerging as a new potential therapeutic target for the treatment of patients with major depressive disorder. Copyright © 2015 Elsevier Masson SAS. All rights reserved.

  16. The psychostimulant modafinil enhances gap junctional communication in cortical astrocytes.

    Science.gov (United States)

    Liu, Xinhe; Petit, Jean-Marie; Ezan, Pascal; Gyger, Joël; Magistretti, Pierre; Giaume, Christian

    2013-12-01

    Sleep-wake cycle is characterized by changes in neuronal network activity. However, for the last decade there is increasing evidence that neuroglial interaction may play a role in the modulation of sleep homeostasis and that astrocytes have a critical impact in this process. Interestingly, astrocytes are organized into communicating networks based on their high expression of connexins, which are the molecular constituents of gap junction channels. Thus, neuroglial interactions should also be considered as the result of the interplay between neuronal and astroglial networks. Here, we investigate the effect of modafinil, a wakefulness-promoting agent, on astrocyte gap junctional communication. We report that in the cortex modafinil injection increases the expression of mRNA and protein of connexin 30 but not those of connexin 43, the other major astroglial connexin. These increases are correlated with an enhancement of intercellular dye coupling in cortical astrocytes, which is abolished when neuronal activity is silenced by tetrodotoxin. Moreover, gamma-hydroxybutyric acid, which at a millimolar concentration induces sleep, has an opposite effect on astroglial gap junctions in an activity-independent manner. These results support the proposition that astroglia may play an important role in complex physiological brain functions, such as sleep regulation, and that neuroglial networking interaction is modified during sleep-wake cycle. This article is part of the Special Issue Section entitled 'Current Pharmacology of Gap Junction Channels and Hemichannels'. Copyright © 2013. Published by Elsevier Ltd.

  17. Josephson junctions and circle maps

    Energy Technology Data Exchange (ETDEWEB)

    Bak, P; Bohr, T; Jensen, M H; Christiansen, P V

    1984-01-01

    The return map of a differential equation for the current driven Josephson junction, or the damped driven pendulum, is shown numerically to be a circle map. Phase locking, noise and hysteresis, can thus be understood in a simple and coherent way. The transition to chaos is related to the development of a cubic inflection point. Recent theoretical results on universal behavior at the transition to chaos can readily be checked experimentally by studying I-V characteristics. 17 references, 1 figure.

  18. Squeezed States in Josephson Junctions.

    Science.gov (United States)

    Hu, X.; Nori, F.

    1996-03-01

    We have studied quantum fluctuation properties of Josephson junctions in the limit of large Josephson coupling energy and small charging energy, when the eigenstates of the system can be treated as being nearly localized. We have considered(X. Hu and F. Nori, preprints.) a Josephson junction in a variety of situations, e.g., coupled to one or several of the following elements: a capacitor, an inductor (in a superconducting ring), and an applied current source. By solving an effective Shrödinger equation, we have obtained squeezed vacuum (coherent) states as the ground states of a ``free-oscillating'' (linearly-driven) Josephson junction, and calculated the uncertainties of its canonical momentum, charge, and coordinate, phase. We have also shown that the excited states of the various systems we consider are similar to the number states of a simple harmonic oscillator but with different fluctuation properties. Furthermore, we have obtained the time-evolution operators for these systems. These operators can make it easier to calculate the time-dependence of the expectation values and fluctuations of various quantities starting from an arbitrary initial state.

  19. Superconducting tunnel-junction refrigerator

    International Nuclear Information System (INIS)

    Melton, R.G.; Paterson, J.L.; Kaplan, S.B.

    1980-01-01

    The dc current through an S 1 -S 2 tunnel junction, with Δ 2 greater than Δ 1 , when biased with eV 1 +Δ 2 , will lower the energy in S 1 . This energy reduction will be shared by the phonons and electrons. This device is shown to be analogous to a thermoelectric refrigerator with an effective Peltier coefficient π* approx. Δ 1 /e. Tunneling calculations yield the cooling power P/sub c/, the electrical power P/sub e/ supplied by the bias supply, and the cooling efficiency eta=P/sub c//P/sub e/. The maximum cooling power is obtained for eV= +- (Δ 2 -Δ 1 ) and t 1 =T 1 /T/sub c/1 approx. 0.9. Estimates are made of the temperature difference T 2 -T 1 achievable in Al-Pb and Sn-Pb junctions with an Al 2 O 3 tunneling barrier. The performance of this device is shown to yield a maximum cooling efficiency eta approx. = Δ 1 /(Δ 2 -Δ 1 ) which can be compared with that available in an ideal Carnot refrigerator of eta=T 1 /(T 2 -T 1 ). The development of a useful tunnel-junction refrigerator requires a tunneling barrier with an effective thermal conductance per unit area several orders of magnitude less than that provided by the A1 2 O 3 barrier in the Al-Pb and Sn-Pb systems

  20. Theoretical modeling and optimization of III-V GaInP/GaAs/Ge monolithic triple-junction solar cells

    International Nuclear Information System (INIS)

    Leem, Jung Woo; Yu, Jae Su; Kim, Jong Nam; Noh, Sam Kyu

    2014-01-01

    We design and optimize monolithic III-V GaInP/GaAs/Ge triple-junction (TJ) solar cells by using a commercial software Silvaco ATLAS simulator to obtain the maximum short-circuit current density J sc . The maximum J sc , which is a current matching value between the GaInP top and GaAs middle subcells, can be determined by varying the base thicknesses of the GaInP top and GaAs middle subcells. From the numerical simulation results, a matched maximum J sc value of 13.92 mA/cm 2 is obtained at base thicknesses of 0.57 μm and 3 μm for the GaInP top and GaAs middle subcells, respectively, under 1-sun air mass 1.5 global spectrum illumination, leading to a high power conversion efficiency of 30.72%. The open-circuit voltage and the fill factor are 2.55 V and 86.55%, respectively. For the optimized cell structure, the external quantum efficiency and the photogeneration rate distributions are also investigated. To obtain efficient antireflection coatings (ARCs), we perform optical reflectance calculations by using a rigorous coupled-wave analysis method. For this, a silicon oxide/titanium oxide double-layer is used as an ARC on the TJ solar cell.