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Sample records for juice morinda citrifolia

  1. Microorganisms and antifungal properties associated with noni (Morinda citrifolia) fruit and fermented juice in Hawaii

    Science.gov (United States)

    Noni (Morinda citrifolia), a medicinal plant grown in Hawaii and other Polynesian regions, is reportedly therapeutic for diabetes, high blood pressure, and certain types of cancer. Noni fruit often produce fermented juice that differs in chemical, physical and microbial properties. To determine ho...

  2. Preventive effect of Morinda citrifolia fruit juice on neuronal damage induced by focal ischemia.

    Science.gov (United States)

    Harada, Shinichi; Hamabe, Wakako; Kamiya, Kohei; Satake, Toshiko; Yamamoto, Junichiro; Tokuyama, Shogo

    2009-03-01

    It is known that the fruit juice of Morinda citrifolia (M. citrifolia, Noni, Rubiaceae) has various pharmacological effects such as antioxidant or anti-inflammatory activities, which may help the inhibition of ischemic neuronal damage. Here, we examined the effect of the fruit juice of M. citrifolia (Noni juice) on the brain damage caused by ischemic stress in mice. Noni juice was obtained from the mature fruit grown in Okinawa (about 1.5 l/4 kg of fruit; 100% Okinawa Noni juice (ONJ). Male ddY mice were supplied with 3% or 10% juice in the drinking water for 7 d, and compared to the control group. On the 7th day, mice were subjected to 2 h of middle cerebral artery occlusion (MCAO). Interestingly, the intake of juice reduced the infarct volume as analyzed by 2,3,5-triphenyltetrazolium chloride (TTC) staining on the 3rd day of MCAO when compared to the control group. Furthermore, we found that the neurological deficit scores (NDS) were decreased after the reperfusion in the juice-supplied mice. On the other hand, the intake of juice did not affect the expression levels of antioxidant such as Cu/Zn superoxide dismutase. The present study suggests that Noni juice may have a preventive effect against cerebral ischemic stress, while further studies are needed to explain the detailed mechanism.

  3. Drug-Induced Liver Injury Associated with Noni (Morinda citrifolia) Juice and Phenobarbital

    Science.gov (United States)

    Mrzljak, Anna; Kosuta, Iva; Skrtic, Anita; Kanizaj, Tajana Filipec; Vrhovac, Radovan

    2013-01-01

    Noni (Morinda citrifolia) juice is a popular herbal dietary supplement globally used for preventive or therapeutic purposes in a variety of ailments, claiming to exhibit hepatoprotective properties as well. Herein we present the case of a 38-year-old woman who developed acute liver injury associated with noni juice consumption on a long-term (9 months) anticonvulsant therapy. Clinical presentation and liver biopsy were consistent with severe, predominantly hepatocellular type of injury. Both agents were stopped and corticosteroids were initiated. Five months later the patient had fully recovered. Although in the literature the hepatotoxicity of noni juice remains speculative, sporadic but emerging cases of noni juice-associated liver injury address the need to clarify and investigate potential harmful effects associated with this supplement. PMID:23467452

  4. Drug-Induced Liver Injury Associated with Noni (Morinda citrifolia) Juice and Phenobarbital.

    Science.gov (United States)

    Mrzljak, Anna; Kosuta, Iva; Skrtic, Anita; Kanizaj, Tajana Filipec; Vrhovac, Radovan

    2013-01-01

    Noni (Morinda citrifolia) juice is a popular herbal dietary supplement globally used for preventive or therapeutic purposes in a variety of ailments, claiming to exhibit hepatoprotective properties as well. Herein we present the case of a 38-year-old woman who developed acute liver injury associated with noni juice consumption on a long-term (9 months) anticonvulsant therapy. Clinical presentation and liver biopsy were consistent with severe, predominantly hepatocellular type of injury. Both agents were stopped and corticosteroids were initiated. Five months later the patient had fully recovered. Although in the literature the hepatotoxicity of noni juice remains speculative, sporadic but emerging cases of noni juice-associated liver injury address the need to clarify and investigate potential harmful effects associated with this supplement.

  5. Drug-Induced Liver Injury Associated with Noni (Morinda citrifolia Juice and Phenobarbital

    Directory of Open Access Journals (Sweden)

    Anna Mrzljak

    2013-01-01

    Full Text Available Noni (Morinda citrifolia juice is a popular herbal dietary supplement globally used for preventive or therapeutic purposes in a variety of ailments, claiming to exhibit hepatoprotective properties as well. Herein we present the case of a 38-year-old woman who developed acute liver injury associated with noni juice consumption on a long-term (9 months anticonvulsant therapy. Clinical presentation and liver biopsy were consistent with severe, predominantly hepatocellular type of injury. Both agents were stopped and corticosteroids were initiated. Five months later the patient had fully recovered. Although in the literature the hepatotoxicity of noni juice remains speculative, sporadic but emerging cases of noni juice-associated liver injury address the need to clarify and investigate potential harmful effects associated with this supplement.

  6. Hypoglycemic and Hepatoprotective Activity of Fermented Fruit Juice of Morinda citrifolia (Noni in Diabetic Rats

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    B. Shivananda Nayak

    2011-01-01

    Full Text Available Morinda citrifolia is a medicinal plant used to treat diabetes and liver diseases. The fermented fruit juice of the M. Citrifolia (optical density = 1.25 was used to study the hypoglycemic and hepatoprotective properties in diabetes-induced rats. The rats were randomly distributed into 4 groups (control, diabetic experimental, diabetic standard, and diabetic untreated of 6 each. Diabetes was induced by administering Streptozotocin (50 mg/kg body weight. Fasting blood glucose, body mass, liver tissue glycogen content, and the extent of liver degeneration were assessed. Diabetic experimental animals were treated with M. citrifolia juice (2 ml/kg, twice a day and diabetic standard with reference hypoglycemic drug, glibenclamide orally for 20 days. Both the groups exhibited a significant reduction in blood glucose level of 150 mg/dl ±15.88 and 125 mg/dl ±3.89, respectively, as compared to diabetic untreated with FBS = 360.0 mg/dl ±15.81, (<.003. On 10th day of experiment, diabetic experimental animals exhibited a decrease in body mass (10.2 g, 5.11% which increased significantly by the 20th day (6 g, 3.0%, <.022. Histological study of liver tissue obtained from untreated diabetic animals revealed significant fatty degeneration as compared to other three groups. The data of this study proved the hypoglycemic and hepatoprotective activity of M. citrifolia.

  7. Postharvest ripening of noni fruit (Morinda citrifolia) and the microbial and chemical properties of its fermented juice

    Science.gov (United States)

    Noni (Morinda citrifolia) is a tropical plant used traditionally in Polynesia, Southeast Asia and other regions for medicinal purposes. Noni fruit and juice extracts are reportedly therapeutic for diabetes, high blood pressure, and certain types of cancer. Research was conducted to determine the phy...

  8. Extraction and Characterization of Antioxidant Compositions From Fermented Fruit Juice of Morinda citrifolia(Noni)

    Institute of Scientific and Technical Information of China (English)

    LIU Chang-hong; XUE Ya-rong; YE Yong-hang; YUAN Feng-feng; LIU Jun-yan; SHUANG Jing-lei

    2007-01-01

    Extraction and characterization of antioxidative compositions from the extracts of fermented Xisha Noni(Morinda citrifolia L.)juice were studied.The antioxidative constituents of 184.6g freeze-dried extracts of naturally fermented Xisha Noni juice were isolated successfully by petroleum ether,EtOAc and n-BuOH solvents,and the antioxidative effects were measured according to scavenging activity against hydroxyl generated in Fenton reaction system and superoxide anion radicals in pyrogallol autoxidation system.The EtOAc extract exhibited most significantly higher(P<0.01)antioxidative activity than mannitol or vitamin C,while the petroleum ether and n-BuOH extracts showed lower activities compared to were isolated from the EtOAc extract by several chromatography techniques for the first time.The results suggest that several compounds,in particular,the phenolic compounds,contribute separately or synergistically to the antioxidative activity of fermented Noni fruit juice.

  9. Ultrastructural Changes and Death of Leishmania infantum Promastigotes Induced by Morinda citrifolia Linn. Fruit (Noni Juice Treatment

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    Fernando Almeida-Souza

    2016-01-01

    Full Text Available The search for new treatments against leishmaniasis has increased due to high frequency of drug resistance registered in endemics areas, side effects, and complications caused by coinfection with HIV. Morinda citrifolia Linn., commonly known as Noni, has a rich chemical composition and various therapeutic effects have been described in the literature. Studies have shown the leishmanicidal activity of M. citrifolia; however, its action on the parasite has not yet been elucidated. In this work, we analyzed leishmanicidal activity and ultrastructural changes in Leishmania infantum promastigotes caused by M. citrifolia fruit juice treatment. M. citrifolia fruit extract showed a yield of 6.31% and high performance liquid chromatography identified phenolic and aromatic compounds as the major constituents. IC50 values were 260.5 µg/mL for promastigotes and 201.3 µg/mL for intracellular amastigotes of L. infantum treated with M. citrifolia. Cytotoxicity assay with J774.G8 macrophages showed that M. citrifolia fruit juice was not toxic up to 2 mg/mL. Transmission electron microscopy showed cytoplasmic vacuolization, lipid inclusion, increased exocytosis activity, and autophagosome-like vesicles in L. infantum promastigotes treated with M. citrifolia fruit juice. M. citrifolia fruit juice was active against L. infantum in the in vitro model used here causing ultrastructural changes and has a future potential for treatment against leishmaniasis.

  10. Ultrastructural Changes and Death of Leishmania infantum Promastigotes Induced by Morinda citrifolia Linn. Fruit (Noni) Juice Treatment.

    Science.gov (United States)

    Almeida-Souza, Fernando; Taniwaki, Noemi Nosomi; Amaral, Ana Cláudia Fernandes; de Souza, Celeste da Silva Freitas; Calabrese, Kátia da Silva; Abreu-Silva, Ana Lúcia

    2016-01-01

    The search for new treatments against leishmaniasis has increased due to high frequency of drug resistance registered in endemics areas, side effects, and complications caused by coinfection with HIV. Morinda citrifolia Linn., commonly known as Noni, has a rich chemical composition and various therapeutic effects have been described in the literature. Studies have shown the leishmanicidal activity of M. citrifolia; however, its action on the parasite has not yet been elucidated. In this work, we analyzed leishmanicidal activity and ultrastructural changes in Leishmania infantum promastigotes caused by M. citrifolia fruit juice treatment. M. citrifolia fruit extract showed a yield of 6.31% and high performance liquid chromatography identified phenolic and aromatic compounds as the major constituents. IC50 values were 260.5 µg/mL for promastigotes and 201.3 µg/mL for intracellular amastigotes of L. infantum treated with M. citrifolia. Cytotoxicity assay with J774.G8 macrophages showed that M. citrifolia fruit juice was not toxic up to 2 mg/mL. Transmission electron microscopy showed cytoplasmic vacuolization, lipid inclusion, increased exocytosis activity, and autophagosome-like vesicles in L. infantum promastigotes treated with M. citrifolia fruit juice. M. citrifolia fruit juice was active against L. infantum in the in vitro model used here causing ultrastructural changes and has a future potential for treatment against leishmaniasis.

  11. Ultrastructural Changes and Death of Leishmania infantum Promastigotes Induced by Morinda citrifolia Linn. Fruit (Noni) Juice Treatment

    Science.gov (United States)

    Almeida-Souza, Fernando; Taniwaki, Noemi Nosomi; Amaral, Ana Cláudia Fernandes; de Souza, Celeste da Silva Freitas; Calabrese, Kátia da Silva; Abreu-Silva, Ana Lúcia

    2016-01-01

    The search for new treatments against leishmaniasis has increased due to high frequency of drug resistance registered in endemics areas, side effects, and complications caused by coinfection with HIV. Morinda citrifolia Linn., commonly known as Noni, has a rich chemical composition and various therapeutic effects have been described in the literature. Studies have shown the leishmanicidal activity of M. citrifolia; however, its action on the parasite has not yet been elucidated. In this work, we analyzed leishmanicidal activity and ultrastructural changes in Leishmania infantum promastigotes caused by M. citrifolia fruit juice treatment. M. citrifolia fruit extract showed a yield of 6.31% and high performance liquid chromatography identified phenolic and aromatic compounds as the major constituents. IC50 values were 260.5 µg/mL for promastigotes and 201.3 µg/mL for intracellular amastigotes of L. infantum treated with M. citrifolia. Cytotoxicity assay with J774.G8 macrophages showed that M. citrifolia fruit juice was not toxic up to 2 mg/mL. Transmission electron microscopy showed cytoplasmic vacuolization, lipid inclusion, increased exocytosis activity, and autophagosome-like vesicles in L. infantum promastigotes treated with M. citrifolia fruit juice. M. citrifolia fruit juice was active against L. infantum in the in vitro model used here causing ultrastructural changes and has a future potential for treatment against leishmaniasis. PMID:27313649

  12. Anti-inflammatory and Quinone Reductase Inducing Compounds from Fermented Noni (Morinda citrifolia) Juice Exudates.

    Science.gov (United States)

    Youn, Ui Joung; Park, Eun-Jung; Kondratyuk, Tamara P; Sang-Ngern, Mayuramas; Wall, Marisa M; Wei, Yanzhang; Pezzuto, John M; Chang, Leng Chee

    2016-06-24

    A new fatty acid ester disaccharide, 2-O-(β-d-glucopyranosyl)-1-O-(2E,4Z,7Z)-deca-2,4,7-trienoyl-β-d-glucopyranose (1), a new ascorbic acid derivative, 2-caffeoyl-3-ketohexulofuranosonic acid γ-lactone (2), and a new iridoid glycoside, 10-dimethoxyfermiloside (3), were isolated along with 13 known compounds (4-16) from fermented noni fruit juice (Morinda citrifolia). The structures of the new compounds, together with 4 and 5, were determined by 1D and 2D NMR experiments, as well as comparison with published values. Compounds 2 and 7 showed moderate inhibitory activities in a TNF-α-induced NF-κB assay, and compounds 4 and 6 exhibited considerable quinone reductase-1 (QR1) inducing effects.

  13. A multigeneration reproductive and developmental safety evaluation of authentic Morinda citrifolia (noni) juice.

    Science.gov (United States)

    Wang, Mian-Ying; Hurn, Jenae; Peng, Lin; Nowicki, Diane; Anderson, Gary

    2011-01-01

    The impact of Morinda citrifolia (noni) juice on fertility and offspring health in three generations of ICR mice was evaluated. The authenticity of the source of noni juice in this study was determined by chemical analysis of known marker compounds. Mice were supplied with 5% noni juice at gestation (day 0) until weaning (21 days postpartum). This procedure was followed through three generations of offspring. Three generations of control mice were also evaluated. There were no intergroup differences in gestation and fertility indices or malformation rates. However, litter sizes of the noni group in the first (F1),  second (F2), and third (F3) generations were, respectively, 29.3% (P < 0.01), 19.8% (P < 0.01) and 19.6% (P < 0.01) larger than corresponding controls. Despite larger litter sizes, there were no decreases in fetal weight in any generation of the noni group. Further, maternal health and offspring viability in the noni groups were equal to or greater than the controls. The results of this study suggest that authentic noni juice has no adverse effect on fertility and fetal development, consistent with previous two-generation studies of noni fruit from French Polynesia, Indonesia, and Hainan , China. On the contrary, noni juice appears to facilitate pregnancy and fetal development.

  14. Pulmonary anti-inflammatory effects and spasmolytic properties of Costa Rican noni juice (Morinda citrifolia L.).

    Science.gov (United States)

    Dussossoy, Emilie; Bichon, Florence; Bony, Emilie; Portet, Karine; Brat, Pierre; Vaillant, Fabrice; Michel, Alain; Poucheret, Patrick

    2016-11-04

    Morinda citrifolia L. (Noni) is a medicinal plant used in Polynesia for many properties such as anti-inflammatory, anti-diabetic and antineoplastic effects. Recent studies showed that noni juice have anti-oxidant and acute anti-inflammatory activities likely due to polyphenols, iridoids and vitamin C content. The present study was undertaken to evaluate chronic anti-inflammatory and spasmolytic effects of noni juice. Therefore, we evaluated the effect of oral or intraperitoneal administrations of noni juice in vivo on the lung inflammation in ovalbumin (OVA) sensitized Brown Norway rat (with prednisolone 10mg/kg intraperitoneously as reference compound) and the ex vivo effect of noni juice on BaCl2 (calcium signal) or methacholine (cholinergic signal) induced spasms in jejunum segments. We found that noni juice (intraperitoneously 2.17mL/kg and orally 4.55mL/kg) reduced the inflammation in OVA-sensitized Brown Norway rat with regard to the decreased number of inflammatory cells in lung (macrophages minus 20-26%, lymphocytes minus 58-34%, eosinophils minus 53-30%, neutrophils minus 70-28% respectively). Noni juice demonstrated a dose-dependent NO scavenging effect up to 8.1nmol of nitrites for 50µL of noni juice. In addition noni juice inhibited (up to 90%) calcium and cholinergic induced spasms on the jejunum segments model with a rightward shift of the concentration response curve. We describe for the first time that noni juice demonstrate (1) a chronic anti-inflammatory activity on sensitized lungs along with (2) a spasmolytic effect integrating a calcium channel blocker activity component. Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.

  15. Total polyphenol content and antioxidant activity of commercial Noni (Morinda citrifolia L. juice and its components

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    Adriana Bramorski

    2010-12-01

    Full Text Available The plant Morinda citrifolia L. (noni has been the focus of many recent studies due to its potential effects on treatment and prevention of several diseases. However, there are few in vivo and in vitro studies concerning its composition and antioxidant capacity. The aim of the present study was to determine the total polyphenol content (TPC and antioxidant capacity of a juice commercialized as noni juice, but containing grape, blueberry and noni fruits. Commercial noni juice was compared against its separate constituents of blueberry and grape juice. Folin-Ciocalteu and DPPH• methods were used to determine the concentration of total polyphenol content and antioxidant activity, respectively. Commercial noni juice presented higher values of TPC (91.90 mg of gallic acid/100 mL juice and antioxidant activity (5.85 mmol/L compared to its 5% diluted constituents. Concentrated blueberry juice presented higher TPC and antioxidant activity than the other juices analyzed. Considering that the blueberry and grape juices account for only 10% in the composition of commercial noni juice, it can be inferred that these two components contribute significantly to the antioxidant activity. Therefore, additional studies are necessary in order to elucidate the contribution of the noni juice as an antioxidant.A planta Morinda citrifolia L. tem sido objeto de muitas pesquisas decorrente de seus efeitos benéficos no tratamento e prevenção de muitas doenças. No entanto, são escassos os estudos in vivo e in vitro sobre os compostos presentes e sua capacidade de atuar como antioxidante. Objetivou-se com este trabalho determinar o índice de polifenóis totais (IPT e a capacidade antioxidante do suco de noni comercial, constituído de uva, mirtilo e a fruta do noni. O suco de noni comercial foi comparado com seus constituintes (mirtilo e suco de uva separadamente. Os métodos Folin-Ciocalteu e DPPH• foram utilizados para determinar a concentração de polifen

  16. The transversal strength of acrylic resin plate after being immersed soaking in noni fruit (Morinda citrifolia Linn. juice

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    Sri Redjeki Indiani

    2008-06-01

    Full Text Available The disadvantages of acrylic resin plate are liquid absorption and porosity, allowing microorganisms to grow and multiply resulting in inflammation in the oral cavity. The juice of the noni fruit (Morinda citrifolia Linn. contains active flavonoid and atsiri oil. Flavonoid is a phenol substance that degrades acrylic resin plate in prolonged contact. The purpose of this study was to examine the transversal strength of acrylic resin plate after being immersed in noni fruit juice. An acrylic resin plate of 65 × 10 × 2.5 mm was immersed in 4%, 6%, 8%, 10%, and 12% concentrations of noni fruit juice and distilled water in a control group for 31, 46, and 61 days. The transversal strength of acrylic resin plate was tested using an autograph with a crosshead speed of 1/10mm/second; the distance for the two supporting parts was 50 mm. The data was analyzed by using a One–Way ANOVA test. There was no significant difference in the transversal strength of the plate after being soaked for 31, 46, and 61 days in 4%, 6%, 8%, 10%, and 12% concentrations of the juice. This study showed that the soaking of acrylic resin plate for 31, 46, and 61 days in 4%, 6%, 8%, 10%, and 12% concentrations of noni fruit juice does not decrease the transversal strength.

  17. Morinda citrifolia fruit juice prevents ischemic neuronal damage through suppression of the development of post-ischemic glucose intolerance.

    Science.gov (United States)

    Harada, Shinichi; Fujita-Hamabe, Wakako; Kamiya, Kohei; Mizushina, Yoshiyuki; Satake, Toshiko; Tokuyama, Shogo

    2010-10-01

    Fruit juice of Morinda citrifolia (Noni juice) is a well-known health drink and has various pharmacological properties including antioxidant and anti-inflammatory effects. We have hitherto found the protective effect of Noni juice on brain damage caused by ischemic stress in mice. In addition, we also recently reported that regulation of post-ischemic glucose intolerance might be important for good prognosis. Here, we focused on the effect of Noni juice on the development of the post-ischemic glucose intolerance as a cerebral protective mechanism. Noni juice was obtained from the mature fruit grown in Okinawa (about 1.5 L/4 kg of fruit; 100% ONJ). Male ddY mice were given 10% ONJ in drinking water for 7 days. Then, mice were subjected to 2 h of middle cerebral artery occlusion (MCAO). Ingestion of 10% ONJ suppressed the development of neuronal damage after MCAO. Interestingly, glucose intolerance observed on the 1st day after MCAO completely disappeared after 10% ONJ administration. Furthermore, ONJ treatment significantly increased serum insulin levels much further than the control group on the 1st day, while serum adiponectin levels were not affected at all. These results suggest that ONJ could facilitate insulin secretion after ischemic stress and may attenuate the development of glucose intolerance. These mechanisms may contribute to the neuronal protective effect of ONJ against ischemic stress.

  18. Cancer preventive effect of Morinda citrifolia (Noni).

    Science.gov (United States)

    Wang, M Y; Su, C

    2001-12-01

    Morinda citrifolia (Noni) has been extensively used in folk medicine by Polynesians for over 2,000 years. It has been reported to have broad therapeutic effects, including anticancer activity, in both clinical practice and laboratory animal models. The mechanism for these effects remains unknown. The hypothesis that Morinda citrifolia possesses a cancer preventive effect at the initiation stage of carcinogenesis was studied. Our preliminary data indicated that 10% Tahitian Noni Liquid Dietary Supplement or Tahitian Noni Juice (TNJ), made from Morinda citrifolia fruit by Morinda Inc, in drinking water for one week was able to prevent DMBA-DNA adduct formation. The levels of DMBA-DNA adducts were reduced by 30% in the heart, 41% in the lung, 42% in the liver, and 80% in the kidney of female SD rats. Even more dramatic results were obtained in male C57 BL-6 mice: 10% TNJ was able to reduce DMBA-DNA adduct formation by 60% in the heart, 50% in the lung, 70% in the liver, and 90% in the kidney. In order to explore the mechanism of this preventive effect, the antioxidant activity of TNJ was examined in vitro by lipid hydroperoxide (LPO) and tetrazolium nitroblue (TNB) assays. In the LPO assay, LPO oxidizes leucomethylene blue to methylene blue in the presence of hemoglobin. The resultant blue color was quantified at 660 nm spectrophotometrically. In the TNB assay, superoxide anion radicals (SAR) reduce TNB into formazan blue that was also measured by absorption at 602 nm. TNJ showed a dose-dependent inhibition of both LPO and SAR in our system. The antioxidant activity of TNJ was compared to the effects of vitamin C, grape seed powder (GSP), and pycnogenol (PYC) at the daily dose per serving level recommended by U.S.RDAs or manufacturers. The results suggest that prevention of carcinogen-DNA adduct formation and the antioxidant activity of TNJ may contribute to the cancer preventive effect of Morinda citrifolia.

  19. Ultrastructural Changes and Death of Leishmania infantum Promastigotes Induced by Morinda citrifolia Linn. Fruit (Noni) Juice Treatment

    OpenAIRE

    Fernando Almeida-Souza; Noemi Nosomi Taniwaki; Ana Cláudia Fernandes Amaral; Celeste da Silva Freitas de Souza; Kátia da Silva Calabrese; Ana Lúcia Abreu-Silva

    2016-01-01

    The search for new treatments against leishmaniasis has increased due to high frequency of drug resistance registered in endemics areas, side effects, and complications caused by coinfection with HIV. Morinda citrifolia Linn., commonly known as Noni, has a rich chemical composition and various therapeutic effects have been described in the literature. Studies have shown the leishmanicidal activity of M. citrifolia; however, its action on the parasite has not yet been elucidated. In this work,...

  20. Noni (Morinda citrifolia Linn.) fruit juice attenuates the rewarding effect of ethanol in conditioned place preference in mice.

    Science.gov (United States)

    Pandy, Vijayapandi; Khan, Yasmin

    2016-11-01

    Morinda citrifolia L. commonly known as noni or Indian mulberry belongs to the family Rubiaceae. Noni fruit juice has recently become a very popular remedy for the treatment of several diseases, including psychiatric disorders. This study aimed to investigate the anticraving effect of Tahitian Noni® Juice (TNJ) against ethanol seeking behavior in ICR male mice using the conditioned place preference (CPP) test. The CPP procedure consisted of four phases: preconditioning, conditioning, extinction, and reinstatement. During conditioning, intraperitoneal (i.p.) injections of ethanol (2 g/kg body weight (bw)) and normal saline (10 ml/kg bw) were given on alternate days for 12 days. Then, the animals were subjected to extinction trials for the next 12 days to weaken CPP. Finally, CPP was reinstated in the extinguished animals by a single low-dose priming injection of ethanol (0.4 g/kg bw, i.p.). The effect of TNJ (as a source of drinking water) on different phases of ethanol CPP in mice was studied. TNJ-treated mice showed a significant reduction in ethanol seeking behavior in the CPP test. The reference drug, acamprosate (ACAM) also showed a similar effect in the CPP test. The outcome of this study suggests that TNJ is effective in attenuating ethanol craving in mice and could be utilized for the treatment of alcohol dependence. Further clinical studies in this direction are warranted to support the present preclinical findings.

  1. Morinda citrifolia Linn. fruit (Noni) juice induces an increase in NO production and death of Leishmania amazonensis amastigotes in peritoneal macrophages from BALB/c.

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    Almeida-Souza, Fernando; de Souza, Celeste da Silva Freitas; Taniwaki, Noemi Nosomi; Silva, João José Mendes; de Oliveira, Renata Mondêgo; Abreu-Silva, Ana Lúcia; Calabrese, Kátia da Silva

    2016-08-31

    Leishmaniasis is a complex disease that is considered a serious public health problem. Due to the absence of an effective vaccine and debilitating chemotherapy better therapies are urgently needed. This situation has stimulated the search for alternative treatments such as the use of herbal medicines. Several studies conducted with Morinda citrifolia Linn. have shown various biological activities such as antitumor, immunomodulation and antileishmanial activity, however its mechanisms of action are still unknown. This study aimed to analyze the activity of M. citrifolia fruit juice against Leishmania amazonensis and its action on peritoneal macrophages from BALB/c infected with L. amazonensis. Activity against the promastigote forms showed IC50 at 275.3 μg/mL. Transmission electron microscopy was used to evaluate the ultrastructural alterations in the promastigotes treated with the juice and the results showed cytoplasmic vacuolization, lipid inclusion and increased activity of exocytosis. The juice treatment presented an IC50 at 208.4 μg/mL against intracellular amastigotes and led to an increased nitrite production in infected and non-infected macrophages. When macrophages were pre-treated with iNOS inhibitors, aminoguanidine or 1400W, the intracellular amastigotes increased, demonstrating the important role of NO production in M. citrifolia fruit activity. In conclusion, our results reveal that treatment with M. citrifolia fruit juice can increase NO production in peritoneal macrophages and this ability has an important role in the killing of L. amazonensis intracellular amastigotes.

  2. MEMPELAJARI KARAKTERISTIK SARI BUAH DARI MENGKUDU (Morinda citrifolia Linn. YANG DIHASILKAN MELALUI FERMENTASI [Characteristic of Noni (Morinda citrifolia Linn Fruit Juice Produced by Fermentation

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    Ivonne P Kusuma3

    2003-08-01

    Full Text Available Fermentation is one of the methods to get noni juice. In this research, noni is fermented in hanged system and submerged system for 1, 2, 3, and 4 weeks. The result showed the longer fermentation increased pH, alcohol content, turbidity, viscosity, microbial content, and decrease ascorbic acid and acid content, soluble solid content, color and flavor acceptance. Differennt system fermentation affected percent yield, soluble solid content, turbidity of juice, panels opinion to color significantly. Noni juice of submerged system has higher percent yield, higher soluble solid content, turbidity, but provided lighter color than noni juice of hanged system. Juice of submerged system is better than hanged system. Juice from three weeks fermentation indicated changes of metabolism activity which is shown by some characteristic changes. Fruit juice made by submerged fermentation system had more diverse compounds than fruit juice without fermentation.

  3. Beneficial effects of noni (Morinda citrifolia L.) juice on livers of high-fat dietary hamsters.

    Science.gov (United States)

    Lin, Yi-Ling; Chang, Yuan-Yen; Yang, Deng-Jye; Tzang, Bor-Show; Chen, Yi-Chen

    2013-09-01

    Polyphenols in noni juice (NJ) are mainly composed of phenolic acids, mainly gentisic, p-hydroxybenoic, and chlorogenic acids. To investigate the beneficial effects of NJ on the liver, hamsters were fed with two diets, normal-fat and high-fat diets. Furthermore, high-fat dietary hamsters were received distilled water, and 3, 6, and 9 mL NJ/kg BW, respectively. After a 6-week feeding period, the increased (p<0.05) sizes of liver and visceral fat in high-fat dietary hamsters compared to the control hamsters were ameliorated (p<0.05) by NJ supplementation. NJ also decreased (p<0.05) serum/liver lipids but enhanced (p<0.05) daily faecal lipid/bile acid outputs in the high-fat dietary hamsters. High-fat dietary hamsters supplemented with NJ had higher (p<0.05) liver antioxidant capacities but lowered (p<0.05) liver iNOS, COX-2, TNF-α, and IL-1β expressions, gelatinolytic levels of MMP9, and serum ALT values compared to those without NJ. Hence, NJ protects liver against a high-fat dietary habit via regulations of antioxidative and anti-inflammatory responses.

  4. First report of association of Mucor circinelloides on noni (Morinda citrifolia) in Hawaii

    Science.gov (United States)

    Noni (Morinda citrifolia) is a popular medicinal plant found in tropical or subtropical regions of the world. The fruit and juice extracts have properties that are reportedly therapeutic for diabetes, high blood pressure, and certain types of cancer (1,4). In our studies on noni juice produced from ...

  5. Antitumour potential of a polysaccharide-rich substance from the fruit juice of Morinda citrifolia (Noni) on sarcoma 180 ascites tumour in mice.

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    Furusawa, Eiichi; Hirazumi, Anne; Story, Stephen; Jensen, Jarakae

    2003-12-01

    An immunomodulatory polysaccharide-rich substance (Noni-ppt) from the fruit juice of Morinda citrifolia has been found to possess both prophylactic and therapeutic potentials against the immunomodulator sensitive Sarcoma 180 tumour system. The antitumour activity of Noni-ppt produced a cure rate of 25%-45% in allogeneic mice and its activity was completely abolished by the concomitant administration of specific inhibitors of macrophages (2-chloroadenosine), T cells (cyclosporine) or natural killer (NK) cells (anti-asialo GM1 antibody). Noni-ppt showed synergistic or additive beneficial effects when combined with a broad spectrum of chemotherapeutic drugs, including cisplatin, adriamycin, mitomycin-C, bleomycin, etoposide, 5- fl uorouracil, vincristine or camptothecin. It was not beneficial when combined with paclitaxel, cytosine arabinoside, or immunosuppressive anticancer drugs such as cyclophosphamide, methotrexate or 6-thioguanine. Noni-ppt also demonstrated beneficial effects when combined with the Th1 cytokine, interferon gamma, but its activity was abolished when combined with Th2 cytokines, interleukin-4 or interleukin-10, thereby suggesting that Noni-ppt induces a Th1 dominant immune status in vivo. The combination of Noni-ppt with imexon, a synthetic immunomodulator, also demonstrated beneficial effects, but not when combined with the MVE-2 copolymer, a high molecular weight immunomodulator. It was also not effective when combined with interleukin-2 or interleukin-12. Copyright 2003 John Wiley & Sons, Ltd.

  6. Morinda citrifolia (Noni Juice Augments Mammary Gland Differentiation and Reduces Mammary Tumor Growth in Mice Expressing the Unactivated c-erbB2 Transgene

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    William P. Clafshenkel

    2012-01-01

    Full Text Available Morinda citrifolia (noni is reported to have many beneficial properties, including on immune, inflammatory, quality of life, and cancer endpoints, but little is known about its ability to prevent or treat breast cancer. To test its anticancer potential, the effects of Tahitian Noni Juice (TNJ on mammary carcinogenesis were examined in MMTV-neu transgenic mice. Mammary tumor latency, incidence, multiplicity, and metastatic incidence were unaffected by TNJ treatment, which suggests that it would not increase or decrease breast cancer risk in women taking TNJ for its other benefits. However, noni may be useful to enhance treatment responses in women with existing HER2/neu breast cancer since TNJ resulted in significant reductions in tumor weight and volume and in longer tumor doubling times in mice. Remarkably, its ability to inhibit the growth of this aggressive form of cancer occurred with the mouse equivalent of a recommended dose for humans (<3 oz/day. A 30-day treatment with TNJ also induced significant changes in mammary secondary ductule branching and lobuloalveolar development, serum progesterone levels, and estrous cycling. Additional studies investigating TNJ-induced tumor growth suppression and modified reproductive responses are needed to characterize its potential as a CAM therapy for women with and without HER2+ breast cancer.

  7. Morinda citrifolia (Noni) Fruit Juice Reduces Inflammatory Cytokines Expression and Contributes to the Maintenance of Intestinal Mucosal Integrity in DSS Experimental Colitis

    Science.gov (United States)

    Coutinho de Sousa, Beatriz; da Costa, Thiago Alvares; Lazo-Chica, Javier Emilio; Degasperi, Thatiane do Prado; Rodrigues Junior, Virmondes; Uber Bucek, Elizabeth

    2017-01-01

    Morinda citrifolia L. (noni) has been shown to treat different disorders. However, data concerning its role in the treatment of intestinal inflammation still require clarification. In the current study, we investigated the effects of noni fruit juice (NFJ) in the treatment of C57BL/6 mice, which were continuously exposed to dextran sulfate sodium (DSS) for 9 consecutive days. NFJ consumption had no impact on the reduction of the clinical signs of the disease or on weight loss. Nonetheless, when a dilution of 1 : 10 was used, the intestinal architecture of the mice was preserved, accompanied by a reduction in the inflammatory infiltrate. Regardless of the concentration of NFJ, a decrease in both the activity of myeloperoxidase and the key inflammatory cytokines, TNF-α and IFN-γ, was also observed in the intestine. Furthermore, when NFJ was diluted 1 : 10 and 1 : 100, a reduction in the production of nitric oxide and IL-17 was detected in gut homogenates. Overall, the treatment with NFJ was effective in different aspects associated with disease progression and worsening. These results may point to noni fruit as an important source of anti-inflammatory molecules with a great potential to inhibit the progression of inflammatory diseases, such as inflammatory bowel disease. PMID:28194046

  8. Morinda citrifolia (Noni Fruit Juice Reduces Inflammatory Cytokines Expression and Contributes to the Maintenance of Intestinal Mucosal Integrity in DSS Experimental Colitis

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    Beatriz Coutinho de Sousa

    2017-01-01

    Full Text Available Morinda citrifolia L. (noni has been shown to treat different disorders. However, data concerning its role in the treatment of intestinal inflammation still require clarification. In the current study, we investigated the effects of noni fruit juice (NFJ in the treatment of C57BL/6 mice, which were continuously exposed to dextran sulfate sodium (DSS for 9 consecutive days. NFJ consumption had no impact on the reduction of the clinical signs of the disease or on weight loss. Nonetheless, when a dilution of 1 : 10 was used, the intestinal architecture of the mice was preserved, accompanied by a reduction in the inflammatory infiltrate. Regardless of the concentration of NFJ, a decrease in both the activity of myeloperoxidase and the key inflammatory cytokines, TNF-α and IFN-γ, was also observed in the intestine. Furthermore, when NFJ was diluted 1 : 10 and 1 : 100, a reduction in the production of nitric oxide and IL-17 was detected in gut homogenates. Overall, the treatment with NFJ was effective in different aspects associated with disease progression and worsening. These results may point to noni fruit as an important source of anti-inflammatory molecules with a great potential to inhibit the progression of inflammatory diseases, such as inflammatory bowel disease.

  9. Postharvest ripening and fermentation of noni fruit (Morinda citrifolia) in Hawaii

    Science.gov (United States)

    Noni (Morinda citrifolia) is a tropical plant used traditionally in Polynesia, Southeast Asia and other regions for medicinal purposes. Noni fruit and juice extracts are reportedly therapeutic for diabetes, high blood pressure, and certain types of cancer. Research was conducted to determine the phy...

  10. Anti-fungal activity of Morinda citrifolia (noni extracts against Candida albicans: An in vitro study

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    K Barani

    2014-01-01

    Full Text Available Aim: The aim of this study was to investigate the anti-fungal activity of Morinda citrifolia fruit extract on Candida albicans. Materials and Methods: Juice extract from M. citrifolia fruit was lyophilized and used in anti-fungal testing. Anti-fungal activity of M. citrifolia fruit extract against C. albicans was tested in vitro at various concentrations. The inhibitory effect of M. citrifolia extract on C. albicans was determined by agar culture and applied broth dilution test. Results: M. citrifolia extract at 1000 μg/ml concentration effectively inhibited the growth of C. albicans (16.6 ± 0.3 compared with the positive control - amphotericin B (20.6 ± 0.6. It was found to be a dose-dependent reaction. Conclusion: M. citrifolia fruit extract had an anti-fungal effect on C. albicans and the inhibitory effect varied with concentration.

  11. Studies of antiviral activity and cytotoxicity of Wrightia tinctoria and Morinda citrifolia

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    Selvam P

    2009-01-01

    Full Text Available Different extracts of leaf parts of Wrightia tinctoria and fruit powder of Morinda citrifolia have been studied against replication of HIV-1(IIIB in MT-4 cells and HCV in Huh 5.2 cells. Chloroform extract of Wrightia tinctoria exhibited a maximum protection of 48% against the cytopathic effect of HIV-1(IIIB in MT-4 cells. Fruit juice of Morinda citrifolia exhibited a displayed marked cytotoxic activity in lymphocyte (MT-4 cells (CC50: 0.19 mg/ml. The 50% effective concentration for inhibition of HCV subgenomic replicon replication in Huh 5-2 cells by Morinda citrifolia was 0.98 µg/ml and by chloroform extract of Wrightia tinctoria was 10 µg/ml. The concentration that reduced the growth of exponentially proliferating Huh 5-2 cells by 50% was greater than 50 µg/ml.

  12. Anticancer activity of Morinda citrifolia (Noni) fruit: a review.

    Science.gov (United States)

    Brown, Amy C

    2012-10-01

    This review investigated the relationship of noni juice, or its extract (fruit, leaves or root), to anticancer and/or immunostimulant properties. A Medline search was conducted using the key search words 'Morinda citrifolia' and 'Morinda citrifolia and cancer' (1964 to October, 2011) along with cross-referencing. Botanical and chemical indexes were not included. A total of 304 and 29 (10%) articles, respectively, were found under these key terms. Of the 19 studies actually related to cancer, seven publications were in vitro cancer studies, nine were in vivo animal cancer studies, and three were in vivo human cancer studies. Among the in vitro studies, a 'concentrated component' in noni juice and not pure noni juice may (1) stimulate the immune system to 'possibly' assist the body fight the cancer, and (2) kill a small percentage (0-36%) of cancer cells depending on the type. The nine animal studies suggest that a concentrated component in noni juice may stimulate the immune system; but only slightly increases the number (about 1/3; 25-45%) of surviving mice. Other than two case studies, only two human clinical studies existed. The first consisted of testing freeze-dried noni fruit, which reduced pain perception, but did not reverse advanced cancer. The second was on smokers ingesting an unknown concentration of noni juice who experienced decreased aromatic DNA adducts, and decreased levels of plasma superoxide anion radicals and lipid hydroperoxide. Factors to consider in the future are clearly defining the substance being tested, and whether or not the juice is pasteurized. Some reports of hepatotoxicity exist, although there were confounding factors in most of the case reports. More importantly, noni juice is high in potassium and needs to be monitored by patients with kidney, liver or heart problems. In conclusion, a few in vitro and in vivo animal studies suggest a possible unidentified substance in unpasteurized noni fruit juice that may have a small degree of

  13. Effect of soaking in noni (Morinda citrifolia) juice on the microbiological and color behavior of Haden minimally processed mango.

    Science.gov (United States)

    Ulloa, José Armando; González Tapia, Noemí T; Rosas Ulloa, Petra; Ramírez Ramírez, José Carmen; Ulloa Rangel, Blanca E

    2015-05-01

    The purpose of this study was to evaluate the effect of soaking in noni juice on the microbiological and color behavior of minimally processed mango. Two batches of Haden mango cubes were treated by immersion in noni juice for 2.5 or 5.0 min. Each batch was packed in polypropylene boxes and stored at 6 °C for up to 15 days; in addition, a control group of mango cubes was prepared by immersion in sterile water for the same duration. According to the results, the soaking of mango cubes in noni juice had an antimicrobial effect on mesophilic aerobic bacteria, molds and yeasts during storage at 6 °C for 15 days, without significantly (P < 0.05) affecting the CIE L*, a*, b*, chroma and hue angle values, in comparison with the control after 12 days of storage. The noni juice soaking treatment was demonstrated to be a potentially valuable technology for decontamination of fresh-cut fruit surfaces.

  14. Chronic toxicity evaluation of Morinda citrifolia fruit and leaf in mice.

    Science.gov (United States)

    Mohamad Shalan, Nor Aijratul Asikin; Mustapha, Noordin M; Mohamed, Suhaila

    2017-02-01

    Noni (Morinda citrifolia) leaf and fruit are used as food and medicine. This report compares the chronic toxicity of Noni fruit and edible leaf water extracts (two doses each) in female mice. The 6 months study showed the fruit extract produced chronic toxicity effects at the high dose of 2 mg/ml drinking water, evidenced through deteriorated liver histology (hepatocyte necrosis), reduced liver length, increased liver injury marker AST (aspartate aminotransferase) and albumin reduction, injury symptoms (hypoactivity, excessive grooming, sunken eyes and hunched posture) and 40% mortality within 3 months. This hepatotoxicity results support the six liver injury reports in humans which were linked to chronic noni fruit juice consumption. Both doses of the leaf extracts demonstrated no observable toxicity. The hepatotoxicity effects of the M. citrifolia fruit extract in this study is unknown and may probably be due to the anthraquinones in the seeds and skin, which had potent quinone reductase inducer activity that reportedly was 40 times more effective than l-sulforaphane. This report will add to current data on the chronic toxicity cases of Morinda citrifolia fruit. No report on the chronic toxicity of Morinda citrifolia fruit in animal model is available for comparison. Copyright © 2016 Elsevier Inc. All rights reserved.

  15. Action of the juice from the fruit of Morinda citrifolia L. on cells of the plant test system

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    Gleuvânia Marques

    2014-03-01

    Full Text Available In this study, we evaluated the action of noni juice on the meristematic cells of Allium cepa roots, at two concentrations, 0.18 and 0.36 mg/mL, with the exposure times of 24 and 48 h. For each concentration, we used a group of 5 onion bulbs, which were first embedded in distilled water and, then, transferred to their respective concentrations. The radicles were collected and fixed in acetic acid (3:1 for 24 hours. The root tips were collected and fixed in acetic acid (3:1 for 24 h. The slides were prepared through the crushing technique, and stained with 2% acetic orcein. Cells were analyzed throughout the cell cycle, totaling 5,000 for each control and exposure time. The mitotic indices calculated underwent chi-square (p < 0.5 statistical analysis. We found out that both concentrations, including the lowest, regarded as ideal for consumption, significantly increased the mitotic index of meristematic cells of onion roots. Therefore, under the analyzed conditions, both tested concentrations of noni juice have promoted change in the cell division of this plant test system.

  16. Hypolipidemic and antioxidative effects of noni (Morinda citrifolia L.) juice on high- fat/cholesterol-dietary hamsters.

    Science.gov (United States)

    Lin, Yi-Ling; Chou, Chung-Hsi; Yang, Deng-Jye; Chen, Jr-Wei; Tzang, Bor-Show; Chen, Yi-Chen

    2012-09-01

    Noni juice (NJ) is rich in phytochemicals and polysaccharides. Lipid-lowering and antioxidative effects of NJ were investigated in this study. Fifty male hamsters were assigned randomly to one of the following groups: (1) normal diet and distilled water (LFCD); (2) high-fat/cholesterol diet and distilled water (HFCD); (3) HFCD and 3 ml NJ (including 0.20 g solids)/kg BW (NJ_L); (4) HFCD and 6 mL NJ (including 0.40 g solids)/kg BW (NJ_M); (5) HFCD and 9 ml NJ (including 0.60 g solids)/kg BW (NJ_H) for six weeks. NJ supplementation decreased (p < 0.05) serum triacylglycerol, cholesterol, atherogenic index, malondialdehyde levels, and hepatic lipids in HFCD hamsters, whereas serum trolox equivalent antioxidant capacity, glutathione, and fecal lipids in HFCD hamsters were increased (p < 0.05) by NJ supplementation. Although NJ supplementation downregulated (p < 0.05) sterol regulator element binding protein-1c in HFCD hamsters, it upregulated (p < 0.05) hepatic peroxisome proliferator-activated receptor-alpha and uncoupling protein 2 gene expressions in HFCD hamsters. Results demonstrate that NJ promotes cardioprotection in a high-fat/cholesterol diet.

  17. Morinda citrifolia leaves enhance osteogenic differentiation and mineralization of human periodontal ligament cells.

    Science.gov (United States)

    Boonanantanasarn, Kanitsak; Janebodin, Kajohnkiart; Suppakpatana, Prapan; Arayapisit, Tawepong; Rodsutthi, Jit-aree; Chunhabundit, Panjit; Boonanuntanasarn, Surintorn; Sripairojthikoon, Wanida

    2014-01-01

    This present study investigated the potential of Morinda citrifolia leaf aqueous extract to induce osteogenic differentiation and matrix mineralization of human periodontal ligament (hPDL) cells. Human periodontal ligament cells were cultured in complete medium, ascorbic acid with β-glycerophosphate, or Morinda citrifolia leaf aqueous extract. Morinda citrifolia leaf aqueous extract significantly increased alkaline phosphatase activity compared to culturing in complete medium or ascorbic acid with β-glycerophosphate. Matrixcontaining mineralized nodules were formed only when the cells were cultured in the presence of Morinda citrifolia leaf aqueous extract. These nodules showed positive alizarin red S staining and were rich in calcium and phosphorus according to energy dispersive X-ray analysis. In conclusion, Morinda citrifolia leaf extract promoted osteogenic differentiation and matrix mineralization in human periodontal ligament cells, a clear indication of the therapeutic potential of Morinda citrifolia leaves in bone and periodontal tissue regeneration.

  18. PENGUKURAN KANDUNGAN SKOPOLETIN PADA BEBERAPA TINGKAT KEMATANGAN BUAH MENGKUDU (Morinda citrifolia Linn) DENGAN METODE KLT DENSITOMETRI

    OpenAIRE

    Diana Nurus Sholehah

    2010-01-01

    Morinda citrifolia fruit has been used as remedies in Indonesia because many diseases could be healed. Scopoletin, one of major component of Morinda citrifolia was chosen as  marker compound because of  its various activites such as  hypotensive, antiinflamatory and antihistamine. The research aimed to determinate scopoletin in some maturations stage of Morinda citrifolia fruit in order to get best fruit for traditional medicine and phytopharmaca with optimal therapeutical effect . A simple a...

  19. Morinda citrifolia Linn. (Rubiaceae) leaf extracts mitigate UVB-induced erythema.

    Science.gov (United States)

    West, Brett J; Deng, Shixin; Palu, Afa K; Jensen, C Jarakae

    2009-07-01

    Morinda citrifolia Linn. (Rubiaceae) leaves have been used in tropical folk medicine to treat topical inflammation and burns. A carbomer gel base, containing the ethanol extract and juice pressed from the leaves, was evaluated for potential allergenic properties in a repeat-insult patch test in 49 volunteers. To investigate the topical photo-protective properties, the combined ethanol extract and leaf juice were evaluated in a UVB-induced erythema model in 25 volunteers. The crude ethanol extract of M. citrifolia leaves was also evaluated in vitro for potential anti-inflammatory activity in a histamine H-1 receptor antagonism assay. There was no evidence of allergenic potential in the repeat-insult patch test. When the combination of ethanol extract and leaf juice was applied, the UVB dose required to induce erythema was almost 3.5 times greater than with untreated skin (P citrifolia leaves inhibited receptor binding by 57%. These results suggest that M. citrifolia leaves are safe for topical use and may be useful in mitigating UVB-induced injury to the skin.

  20. Pengaruh Pemberian Jus Buah Mengkudu (Morinda citrifolia dalam Air Minum Terhadap Penampilan Ayam Broiler Jantan

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    Nurhayati Nurhayati

    2008-04-01

    Full Text Available Effect of noni (Morinda citrifolia fruit juice in drinking water on male broiler chicken performance ABSTRACT. The present study was conducted to determine the effect of Noni (Morinda citrifolia fruit juice in drinking water on male broiler chicken performance. The study used 100 male broiler chicken at 3 days of age strain Platinum MB 202 produced by PT. Multi Breeder Adhirama Indonesia (Grup PT Jafpa Comfeed Indonesia Bandar Lampung. The study was assigned into The Completely Randomized Design with 5 treatments and 4 replications. Chicken were housed in 20 unit colony cages in size 100 x 50 x 50 cm each. The treatments were level of noni fruit juice in drinking water; R0 (control, chicken were not drunk noni fruit juice, R1 (chicken drank water contained 2,5% noni fruit juice, R2 (chicken drank water contained 5% noni fruit juice, R3 (chicken drank water contained 7,5% noni fruit juice and R4 (chicken drank water contained 10% noni fruit juice. Chicken fed similar ration containing 23.58% crude protein and 3943 kkal/kg gross energy. The selected noni fruit were mature fruit or has color white yellowish. Ration and drinking water were offered ad libitum. During the experiment, chicken were vaccinated only by Vaccine ND on 4 days of age. Chicken were kept for 4 weeks. Parameters measured were feed consumption, drinking water consumption, body weight gain and feed conversion ratio. Collected data were analyzed by analysis of variance and significant effect on the parameters were tested by Duncan’s Multiple Range Test. Result of this study showed that there was not significant (P>0,05 effect noni fruit juice in drinking water on feed consumption, drinking water consumption, body weight gain and feed conversion ratio. It is concluded that noni fruit juice could be mixed into the drinking water of broiler chicken up to 10%.

  1. Efficacy of passive ultrasonic irrigation with natural irrigants (Morinda citrifolia juice, Aloe Vera and Propolis in comparison with 1% sodium hypochlorite for removal of E. faecalis biofilm: An in vitro study

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    Anuj Bhardwaj

    2013-01-01

    Full Text Available Aim: Present study evaluated the efficacy of natural derivative irrigants, Morinda citrifolia juice (MCJ, Aloe Vera and Propolis in comparison to 1% sodium hypochlorite with passive ultrasonic irrigation for removal of the intraradicular E. faecalis biofilms in extracted single rooted human permanent teeth. Materials and Methods: Biofilms of E. faecalis were grown on the prepared root canal walls of 60 standardized root halves which were longitudinally sectioned. These root halves were re-approximated and the samples were divided into five groups of twelve each. The groups were, Group A (1% NaOCl, Group B (MCJ, Group C (Aloe vera, Group D (Propolis and Group E (Saline. These groups were treated with passive ultrasonic irrigation (PUI along with the respective irrigants. The root halves were processed for scanning electron microscopy. Three images (X2.5, coronal, middle and apical, were taken for the twelve root halves in each of the five groups. The images were randomized and biofilm coverage assessed independently by three calibrated examiners, using a four-point scoring system. Results: 1% NaOCl with passive ultrasonic irrigation (PUI was effective in completely removing E. faecalis biofilm and was superior to the natural irrigants like MCJ, Aloe vera and Propolis tested in this study. Conclusion: 1% NaOCl used along with passive ultrasonic irrigation was effective in completely removing E. faecalis biofilm when compared to natural irrigants (MCJ, Aloe Vera and Propolis.

  2. Pre-clinical toxicity of Morinda citrifolia Linn. leaf extract

    OpenAIRE

    Serafini, Mairim Russo; Santos, Rodrigo Correia; Dória, Grace Anne Azevedo; Jesus, Josepha Yohanna de; de Melo, Marcelia Garcez Dória; Santos, João Paulo Almeida dos; Rabelo, Thallita Kelly; Fortes, Vanessa Silveira; FONSECA, Maria José Vieira; Albuquerque Junior, Ricardo Luiz Cavalcanti de; Quintans-Júnior, Lucindo José; Araújo, Adriano Antunes de Souza

    2011-01-01

    This investigation was carried out to evaluate the safety of an aqueous extract of Morinda citrifolia leaves (AEMC) by determining its potential toxicity after acute administration (5 g/kg) in rodents and cytotoxic studies. General behavior adverse effects and mortality were determined for up to 14 days. Biochemical and hematological parameters were determined after 14 days. Cytotoxic effects of the AEMC were determined by spectrophotometric 3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazoli...

  3. Anti Epileptic Activity of Morinda citrifolia Linn Fruit Extract

    OpenAIRE

    P. Muralidharan; Srikanth, J

    2010-01-01

    Fruit extract of Morinda citrifolia (Noni), a medicinal plant used in many neuro protective ayurvedic preparations was evaluated for its protective effect against seizures induced by Maximal Electro Shock (MES) method in rats. A daily dose of 200 and 400 mg/kg of the extract was administered to the animals for 15 days, after which seizures were induced by maximum electro shock method and the duration of various phases of epileptic attacks were recorded and compared with the control animals. A...

  4. New anthraquinones from the stem of Morinda citrifolia Linn.

    Science.gov (United States)

    Siddiqui, Bina S; Sattar, Fouzia A; Begum, Sabira; Gulzar, Tahsin; Ahmad, Fayaz

    2006-10-01

    Studies on the chemical constituents of the stem of Morinda citrifolia, Linn. have led to the isolation of two new compounds, morindicinone (=2-hydroxy-1,8-dimethoxy-7-methoxymethylanthraquinone, 1) and morindicininone (=4-hydroxymethyl-1,3-dimethoxyanthraquinone, 2), as well as two known constituents, 2-hydroxyanthraquinone (3) and 2-methoxyanthraquinone (4). Their structures were elucidated by spectral analysis including 2D-NMR techniques.

  5. Chemical Constituents from the Stems of Morinda citrifolia Linn.

    Science.gov (United States)

    Siddiqui, Bina S; Sattar, Fouzia A; Begum, Sabira; Gulzar, Tahsin; Ahmad, Fayaz

    2007-07-01

    Studies on the chemical constituents of the stems of Morinda citrifolia, Linn. have led to the isolation of two new compounds, morindicone (9-hydroxy-2-methoxy-4-methyl-3,10-anthracenedione, 1) and morinthone (4-methoxy-3-heptadecylxanthone, 2), as well as two known constituents, 1-hydroxy-2-methylanthraquinone (3) and 2-hydroxymethylanthraquinone (4). Their structures were elucidated by spectral analysis including 2D NMR techniques.

  6. Hypolipidemic effect of seed oil of noni (Morinda citrifolia).

    Science.gov (United States)

    Pazos, Diana C; Jiménez, Fabiola E; Garduño, Leticia; López, V Eric; Cruz, M Carmen

    2011-07-01

    Morinda citrifolia, has been reported to posses different biological activities and almost all parts of this have been studied phytochemically. However there are few studies on the seeds of fruit. The objective of present study was investigated the effect to Noni Seed Oil (NSO) on serum lipid levels in normolipidemic and hyperlipidemic induced mice. We find that administration of noni oil causes a reduction in total cholesterol and triglycerides levels in both models. However hypolipidemic effect is higher when hyperlipidemia is presented.

  7. Transformation of Morinda citrifolia via simple mature seed imbibition method.

    Science.gov (United States)

    Lee, J J; Ahmad, S; Roslan, H A

    2013-12-15

    Morinda citrifolia, is a valuable medicinal plant with a wide range of therapeutic properties and extensive transformation study on this plant has yet been known. Present study was conducted to establish a simple and reliable transformation protocol for M. citrifolia utilising Agrobacterium tumefaciens via direct seed exposure. In this study, the seeds were processed by tips clipping and dried and subsequently incubated in inoculation medium. Four different parameters during the incubation such as incubation period, bacterial density, temperature and binary vectors harbouring beta-glucuronidase (GUS) gene (pBI121 and pGSA1131), were tested to examine its effect on transformation efficiency. The leaves from the treated and germinated seedlings were analysed via Polymerase Chain Reaction (PCR), histochemical assay of the GUS gene and reverse transcription-PCR (RT-PCR). Results of the study showed that Agrobacterium strain LBA4404 with optical density of 1.0 and 2 h incubation period were optimum for M. citrifolia transformation. It was found that various co-cultivation temperatures tested and type of vector used did not affect the transformation efficiency. The highest transformation efficiency for M. citrifolia direct seed transformation harbouring pBI121 and pGSA1131 was determined to be 96.8% with 2 h co-cultivation treatment and 80.4% when using bacterial density of 1.0, respectively. The transformation method can be applied for future characterization study of M. citrifolia.

  8. The fruit juice of Morinda citrifolia (noni) downregulates HIF-1α protein expression through inhibition of PKB, ERK-1/2, JNK-1 and S6 in manganese-stimulated A549 human lung cancer cells.

    Science.gov (United States)

    Jang, Byeong-Churl

    2012-03-01

    High exposure of manganese is suggested to be a risk factor for many lung diseases. Evidence suggests anticancerous and antiangiogenic effects by products derived from Morinda citrifolia (noni) fruit. In this study, we investigated the effect of noni fruit juice (NFJ) on the expression of HIF-1α, a tumor angiogenic transcription factor in manganese-chloride (manganese)-stimulated A549 human lung carcinoma cells. Treatment with manganese largely induced expression of HIF-1α protein but did not affect HIF-1α mRNA expression in A549 cells, suggesting the metal-mediated co- and/or post-translational HIF-1α upregulation. Manganese treatment also led to increased phosphorylation of extracellular-regulated protein kinase-1/2 (ERK-1/2), c-Jun N-terminal kinase-1 (JNK-1), protein kinase B (PKB), S6 and eukaryotic translation initiation factor-2α (eIF-2α) in A549 cells. Of note, the exposure of NFJ inhibited the manganese-induced HIF-1α protein upregulation in a concentration-dependent manner. Importantly, as assessed by results of pharmacological inhibition and siRNA transfection studies, the effect of NFJ on HIF-1α protein downregulation seemed to be largely associated with the ability of NFJ to interfere with the metal's signaling to activate PKB, ERK-1/2, JNK-1 and S6 in A549 cells. It was further shown that NFJ could repress the induction of HIF-1α protein by desferoxamine or interleukin-1β (IL-1β), another HIF-1α inducer in A549 cells. Thus, the present study provides the first evidence that NFJ has the ability to strongly downregulate manganese-induced HIF-1α protein expression in A549 human lung cancer cells, which may suggest the NFJ-mediated beneficial effects on lung pathologies in which manganese and HIF-1α overexpression play pathogenic roles.

  9. Two new glycosides from the fruits of Morinda citrifolia L.

    Science.gov (United States)

    Hu, Ming-Xu; Zhang, Hong-Cai; Wang, Yu; Liu, Shu-Min; Liu, Li

    2012-01-01

    To study the chemical constituents of the fruits of noni (Morinda citrifolia L.), and find novel compounds, an n-butanol extract of the ethanol soluble fraction was subjected to repeated silica gel and ODS column chromatography and HPLC. Two new glycosides were isolated and their structures elucidated by NMR and HRFAB-MS spectrometry as (2E,4E,7Z)-deca-2,4,7-trienoate-2-O-β-D-glucopyranosyl-β-D-glucopyranoside and amyl-1-O-β-D-apio-furanosyl-1,6-O-β-D-glucopyranoside, respectively.

  10. Two New Glycosides from the Fruits of Morinda citrifolia L.

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    Yu Wang

    2012-10-01

    Full Text Available To study the chemical constituents of the fruits of noni (Morinda citrifolia L., and find novel compounds, an n-butanol extract of the ethanol soluble fraction was subjected to repeated silica gel and ODS column chromatography and HPLC. Two new glycosides were isolated and their structures elucidated by NMR and HRFAB-MS spectrometry as (2E,4E,7Z-deca-2,4,7-trienoate-2-O-β-D-glucopyranosyl-β-D-glucopyra-noside (1 and amyl-1-O-β-D-apio-furanosyl-1,6-O-β-D-glucopyranoside (2, respectively.

  11. Antibacterial Constituents of Hainan Morinda citrifolia (Noni) Leaves.

    Science.gov (United States)

    Zhang, Wei-Min; Wang, Wei; Zhang, Jing-Jing; Wang, Zhi-Rong; Wang, Yu; Hao, Wang-Jun; Huang, Wu-Yang

    2016-05-01

    Noni (Morinda citrifolia L.) is an edible and medicinal plant distributed in Hainan, China. The antibacterial activities of the extracts of water (WE), petroleum ether (PEE), ethyl acetate (EAE), chloroform (CE), and n-butanol (BE) were assayed by the disk diffusion method. The results showed that the extracts from Noni leaves possessed antibacterial effects against Bacillus subtilis, Escherichia coli, Proteus vulgaris, and Staphylococcus aureus. Among 5 different extracts, the BE produced the best antibacterial activity. The samples were first extracted by ethanol, and the primary compounds in the BE fraction of ethanol extract was further isolated and identified. Six phenolic compounds, including 5, 15-dimethylmorindol, ferulic acid, p-hydroxycinamic acid, methyl 4-hydroxybenzoate, methyl ferulate, and methyl 4-hydroxycinnamate, were identifiedby NMR. The results indicated that the phenolic compounds might significantly contribute to antibacterial activities of Noni leaves.

  12. Efficacy of a Morinda citrifolia Based Skin Care Regimen

    Directory of Open Access Journals (Sweden)

    Brett J. West

    2012-04-01

    Full Text Available A six week clinical trial of a Morinda citrifolia (noni based skin care regimen was conducted with 49 women, ages 38 to 55 years. Daily application of three product formulations to the face and neck resulted in significant reductions in lateral canthal fine lines and wrinkles (crow’s feet, as measured by technician scoring and digital image analysis. Use of the regimen also improved skin elasticity and firmness Cutometer® measurements. No evidence of skin irritation was present in any participant at any time during the trial. A study questionnaire revealed that the measured improvements were visibly perceptible to more than 90% of the participants. The trial results substantiate traditional uses of the noni plant to improve skin health.

  13. Toxicity and Antioxidant Tests of Morinda citrifolia (noni Seed Extract

    Directory of Open Access Journals (Sweden)

    Brett J. West

    2011-08-01

    Full Text Available The aim of the current study was to evaluate Morinda citrifolia (noni seed extract, a food ingredient, for potential toxicity and antioxidant activity. Nitrates, nitrites, phytic acid, oxalic acid, as well as aflatoxins B1, B2, G1 and G2 were not detected in the extract. The extract was also non-cytoxic (LC50 > 1 mg/mL in the 24 and 40 h brine shrimp toxicity test. There were no symptoms of toxicity in a subacute (28 day oral toxicity test in Sprague-Dawley rats. Noni seed extract did not display any genotoxic potential in a primary DNA damage test in E. coli PQ37. The extract did exhibit significant antioxidant activity in the Oxygen Radical Absorbance Capacity (ORAC and Ferric Reducing Antioxidant Power (FRAP tests.

  14. Interval Waktu Penambahan Ampas Mengkudu (Morinda citrifolia L. dalam Ransum terhadap Performans Ayam Broiler

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    Yuni Primandini

    2012-04-01

    Full Text Available Time interval of supplementation of Morinda citrifolia L. by product on the performance broiler chicken  ABSTRACT. The research using Completely Randomized Design with 3x2 factorial arrangement and 4 replicated. Dose of Morinda citrifolia by product (T that is 0 g/kg ( T0; 0.50 g/kg ( T1; 0.75 g/kg ( T2 and 1.00 g/kg (T3 as first factor and time interval that is continue (A1 and 1 week interval (A2 as second factor. The performance parameters observed were : feed intake, body weight and small intestine measure. The result showed that no significant effect of time interval and interaction between time interval with Morinda citrifolia by product dose (p>0.05 on feed intake and small intestine measure. The addition dose of Morinda citrifolia by product increased (p<0.05 body weight, there was interaction (p<0.05 between time interval and Morinda citrifolia by product dose on body weight.

  15. Inhibitory effect of morinda citrifolia L. On lipoprotein lipase activity.

    Science.gov (United States)

    Pak-Dek, M S; Abdul-Hamid, A; Osman, A; Soh, C S

    2008-10-01

    Efficacy of Morinda citrifolia L. leaf (MLE) and fruit extracts (MFE) in inhibiting lipoprotein lipase (LPL) was determined in vitro. The result of the study showed that the highest inhibition on the LPL activity was exhibited by MLE (66%+/- 2.1%), which is significantly higher than that demonstrated by MFE (54.5%+/- 2.5%), green tea extract (GTE) (54.5%+/- 2.6%), and catechin (43.6%+/- 6.1%). Percent of LPL inhibition increase with concentration of the extracts. Quantitative analysis of the extracts revealed the presence of high levels of (+)-catechin at 63.5 +/- 17 and 53.7 +/- 5.7 mg/g in MLE and MFE, respectively, although not as high as that found in GTE (530.6 +/- 42 mg/g). Appreciable amount of epicatechin was found in all extracts tested, while rutin was only found in MLE and MFE. The study suggested that both leaf and fruit of M. citrifolia may be used as antiobesity agents in body weight management.

  16. Antipsychotic-like activity of Noni (Morinda citrifolia Linn. in mice

    Directory of Open Access Journals (Sweden)

    Pandy Vijayapandi

    2012-10-01

    Full Text Available Abstract Background Noni fruit is widely consumed in tropical regions of Indonesia to the Hawaiian Islands. The noni plant has a long history of use as a medicinal plant to treat a wide variety of ailments including CNS disorders. The present investigation was designed to evaluate the antipsychotic effect of noni fruits (Morinda citrifolia Linn. using mouse models of apomorphine-induced climbing behaviour and methamphetamine-induced stereotypy (licking, biting, gnawing and sniffing. Methods In acute study, the methanolic extract of Morinda citrifolia (MMC at different doses 1, 3, 5, 10 g/kg was administered orally one hour prior to apomorphine (5 mg/kg, i.p and methamphetamine ( 5 mg/kg, i.p injection respectively in Swiss albino mice. In chronic studies, (TAHITIAN NONI® Juice, TNJ was made available freely in daily drinking water at 30, 50 and 100% v/v for 7 days; 30 and 50% v/v for 21 days respectively. On the test day, an equivalent average daily divided dose of TNJ was administered by oral gavage one hour prior to apomorphine treatment. Immediately after apomorphine/ methamphetamine administration, the animals were placed in the cylindrical metal cages and observed for climbing behaviour/ stereotypy and climbing time. Results The acute treatment of MMC (1, 3, 5, 10 g/kg, p.o significantly decreased the apomorphine-induced cage climbing behaviour and climbing time in mice in a dose dependent manner. The MMC also significantly inhibited methamphetamine-induced stereotypy behaviour and climbing time in mice dose-dependently. The 7 and 21 days treatment of TNJ in drinking water at 50 and 100%v/v significantly alleviated the apomorphine-induced climbing behaviour and climbing time in mice. Conclusions The present study results demonstrated the antidopaminergic effect of Morinda citrifolia Linn. in mice, suggesting that noni has antipsychotic-like activity which can be utilized in the treatment of psychiatric disorders. However further studies

  17. Anthraquinone Content in Noni (Morinda citrifolia L.).

    Science.gov (United States)

    Bussmann, Rainer W; Hennig, Lothar; Giannis, Athanassios; Ortwein, Jutta; Kutchan, Toni M; Feng, Xi

    2013-01-01

    Noni has been used in traditional medicine and as food for thousands of years. While the fruits serve as food and internal medicine, leaves were traditionally used only topically. In recent years, concern regarding the possible content of anthraquinones in noni has led to scrutiny by the European Food Safety Authority. Little research existed on the content of anthraquinones in different noni preparations, with no information about the potential effect of harvest and preparation methods. Our research focused on lucidin, alizarin, and rubiadin, the most important anthraquinones from a health perspective. We found that the production process (fermentation/juice production versus drying/lyophilization) has no effect on the anthraquinone content. The source product, however, does have implications: noni fruit puree from which seeds had been removed as well as consumer products produced from such puree had no detectable amounts of any anthraquinones. Products that did contain seed or leaf material in all cases did contain partly significant amounts of anthraquinones. To alleviate safety concerns, we suggest that noni products, whether fermented or unfermented juice or powder, should be derived only from fully ripe noni fruits, and that any seed material needs to be removed during the production process.

  18. Anthraquinone Content in Noni (Morinda citrifolia L.

    Directory of Open Access Journals (Sweden)

    Rainer W. Bussmann

    2013-01-01

    Full Text Available Noni has been used in traditional medicine and as food for thousands of years. While the fruits serve as food and internal medicine, leaves were traditionally used only topically. In recent years, concern regarding the possible content of anthraquinones in noni has led to scrutiny by the European Food Safety Authority. Little research existed on the content of anthraquinones in different noni preparations, with no information about the potential effect of harvest and preparation methods. Our research focused on lucidin, alizarin, and rubiadin, the most important anthraquinones from a health perspective. We found that the production process (fermentation/juice production versus drying/lyophilization has no effect on the anthraquinone content. The source product, however, does have implications: noni fruit puree from which seeds had been removed as well as consumer products produced from such puree had no detectable amounts of any anthraquinones. Products that did contain seed or leaf material in all cases did contain partly significant amounts of anthraquinones. To alleviate safety concerns, we suggest that noni products, whether fermented or unfermented juice or powder, should be derived only from fully ripe noni fruits, and that any seed material needs to be removed during the production process.

  19. The effect of antibiotic and Morinda citrifolia waste bioactive as feed additive in layer ration

    Directory of Open Access Journals (Sweden)

    I.A. K Bintang

    2008-06-01

    Full Text Available A study on the use of zinc bacitracin antibiotic (ZnB and Morinda citrifolia waste as feed additive in layer ration was conducted. One hundred and twenty pullet of Isa Brown strain were allocated into 5 treatments with 6 replications with 4 birds/replication. The treatment were: control, control + antibiotic (50 ppm Zinc bacitracin and control + M. citrifolia waste at 3 levels (5, 10 and 15 g/kg ration. Analysis of variance was conducted in a completely randomized design. Variables measured were: feed intake, hen day (% HD, egg weight and feed conversion ratio (FCR. The results showed that feed intake of hens with M. citrifolia waste 5g/kg for 6 month was significantly (P0.05. The percentage of HD with antibiotic and M. citrifolia waste did not significantly (P>0.05 differ, but tent to be higher than that of the control. Egg weight with M. citrifolia waste 5 g/kg was significantly (P0.05.differ. FCR with antibiotic and M. citrifolia waste 5 g/kg was significantly lower than that of control. Between antibiotic and M. citrifolia waste was not significantly different (P<0.05, but FCR with antibiotic and M. citrifolia waste 5 g/kg tent to be lower than that of M. citrifolia waste 10 and 15g/kg. It is concluded that the use of M. citrifolia waste 5 g/kg can substituted antibiotic in layer ration.

  20. KOMPISISI MINYAK BIJI MENGKUDU (Morinda citrifolia L. [Oil Composition of Mengkudu (Morinda Citrofolia L Seeds

    Directory of Open Access Journals (Sweden)

    K H Timotius

    2003-12-01

    Full Text Available Mengkudu (Morinda citrifolia L. seeds were macerated or Soxhlet-extracted with various extractants (MeOH, EtAc, Chl : MeOH (2:1, v/v, hexane, and petroleum ether. Fatty acid profile was determined by using GC-MS.The amount of total lipid was varied from 4 - 8 % (w/w of seed weight depends on the extraction method and the polarity of extractant. The obtained major fatty acids were linoleic acid ( 55-63 % of the total fatty acid methyl esters followed by oleic acid, palmitic acid, and stearic acid. Chromatography on a silica column with solvent of increasing polarity yielded 85 % neutral lipids, 10 % glycolipids, and 5 % phospholipids.

  1. Anti Epileptic Activity of Morinda citrifolia Linn Fruit Extract

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    P. Muralidharan

    2010-01-01

    Full Text Available Fruit extract of Morinda citrifolia (Noni, a medicinal plant used in many neuro protective ayurvedic preparations was evaluated for its protective effect against seizures induced by Maximal Electro Shock (MES method in rats. A daily dose of 200 and 400 mg/kg of the extract was administered to the animals for 15 days, after which seizures were induced by maximum electro shock method and the duration of various phases of epileptic attacks were recorded and compared with the control animals. A significant (P<0.01 and P<0.001 reduction in the time taken for righting reflex (recovery was noted in the experimental animals. The levels of biogenic amines such as dopamine, serotonin and nor-adrenaline in the forebrain region were also estimated and a significant level of restoration was opserved in the extract treated animals. Significant results were opserved in the estimated parameters thereby justifying the use of this medicinal plant in the treatment of epilepsy.

  2. Isolation and structural elucidation of chemical constituents from the fruits of Morinda citrifolia Linn.

    Science.gov (United States)

    Siddiqui, Bina S; Sattar, Fouzia A; Ahmad, Fayaz; Begum, Sabira

    2007-08-01

    The fruits of Morinda citrifolia, Linn. afforded a new constituent, morinaphthalenone (1), and three known constituents, scopoletin (2), 1, 3-dimethoxy-anthraquinone (3) and 1, 2-dihydroxy-anthraquinone (4). The structures of these isolated compounds were determined by spectroscopic methods, including 1D- and 2D-NMR (COSY-45, HMQC, HMBC) techniques, as well as by comparison with published values.

  3. Surfactant-induced non-lethal release of anthraquinones from suspension culture of Morinda citrifolia.

    NARCIS (Netherlands)

    Bassetti, L.; Hagendoorn, M.J.M.; Tramper, J.

    1995-01-01

    A new approach based on the use of the surfactant Pluronic F-68 to obtain non-lethal release of plant cell intracellular products was investigated. Suspension cultures of Morinda citrifolia (Rubiaceae), producing anthraquinones as secondary metabolites, were selected as model system. By supplementin

  4. In vitro erythrocyte oxidative damage of Morinda citrifolia L (noni leaves extract

    Directory of Open Access Journals (Sweden)

    Alicia Lagarto

    2013-06-01

    Full Text Available Slight decrease of hemoglobin and erythrocyte count was observed previously after subchronic oral dosing of Morinda citrifolia L leaves extract in rats. Induction of erythrocyte membrane damage could be the cause for these effects. Aims: The objective of this investigation was to assess the in vitro cytotoxicity of Morinda citrifolia L leaves extract and fractions on rat erythrocytes. Methods: Hemolytic damage was assayed in rat erythrocytes. Oxidative stress was assessed by measuring methemoglobin formation, thiobarbituric acid reactive substances (TBARS and enzyme antioxidant activities, superoxide dismutase (SOD and catalase (CAT. Results: Morinda citrifolia L extract caused no hemolysis and induced oxidative damage to red cells in vitro. Methemoglobin increase was observed at concentration between 2 and 8 mg/ml of the extract. Lipid peroxidation was increased and CAT and SOD activities were depleted indicating a possible increase of hydrogen peroxide and superoxide radicals in erythrocytes. Ethyl acetate, dichloromethane and butanol fraction did not cause methemoglobin formation while water fraction increased methemoglobin level at doses up to 6 mg/ml. Conclusions: We concluded that high doses of Morinda citrifolia L extract promote erythrocyte oxidative damage due to metabolites present in water fraction. These could be the cause of decreased erythrocyte and hemoglobin levels observed. [J Intercult Ethnopharmacol 2013; 2(3.000: 135-140

  5. Surfactant-induced non-lethal release of anthraquinones from suspension culture of Morinda citrifolia.

    NARCIS (Netherlands)

    Bassetti, L.; Hagendoorn, M.J.M.; Tramper, J.

    1995-01-01

    A new approach based on the use of the surfactant Pluronic F-68 to obtain non-lethal release of plant cell intracellular products was investigated. Suspension cultures of Morinda citrifolia (Rubiaceae), producing anthraquinones as secondary metabolites, were selected as model system. By

  6. Isolation and detection of taxol, an anticancer drug produced from Lasiodiplodia theobromae, an endophytic fungus of the medicinal plant Morinda citrifolia

    National Research Council Canada - National Science Library

    Mohan Pandi; Rangarajulu Senthil Kumaran; Yong-Keun Choi; Hyung Joo Kim; Johnpaul Muthumary

    2011-01-01

      To determine the production of taxol from an endophytic fungus, Lasiodiplodia theobromae isolated from the medicinal plant Morinda citrifolia and also, to evaluate its cytotoxicity against human...

  7. Probing occurrence of phenylpropanoids in Morinda citrifolia in relation to foliar diseases.

    Science.gov (United States)

    Mandal, Sudhamoy; Rath, Chiranjibi; Gupta, Chandan Kumar; Nath, Vishal; Singh, Hari Shankar

    2015-01-01

    Accumulation of phenolic compounds in cell walls of different plant organs leading to increased lignification is an early defence response of plants against biotic stress. The aim of this work was to delineate occurrence of cell wall-bound (CWB) phenolic compounds in Morinda citrifolia leaves. Alkaline hydrolysis of the cell wall material of leaf tissues yielded 4-coumaric acid (4-CA) as the major bulk of the phenolic compounds in all Morinda germplasms. Next in line was 4-hydroxybenzoic acid. Other phenolics identified were vanillic acid, 4-hydroxybenzaldehyde, vanillin and ferulic acid. Concentrations of all the CWB phenolics were highest in the germplasm CHN-5, followed by the germplasm CHN-1. Incidentally, these two Morinda germplasms recorded lowest incidence of foliar diseases. Significantly higher amounts of 4-CA in combination with other phenolics may be the reasons for lowest incidence of foliar diseases in CHN-5 and CHN-1 germplasms of M. citrifolia.

  8. Interactions of Orthosiphon stamineus and Morinda citrifolia with hepatic aminopyrine metabolism by CYP3A in rats

    Directory of Open Access Journals (Sweden)

    J H Chin

    2009-01-01

    Full Text Available Herb-drug interaction studies have getting attention recently due to the increasingly use of herbal products worldwide. The porpose of the present study was to examine the in vitro effect of methanol leaf extract of Orthosiphon stamineus and Morinda citrofolia fruit juice extract on hepatic aminopyrine metabolism by CYP 3A in different age of normal and STZ-induced diabetic Sprague Dawley (SD rats. Isolated rat hepatocytes were prepared using the collagenase perfusion technique. Aminopyrine was used as a probe substrate to determine the hepatic levels of CYP 3A by measuring the activity of N-demethylation of aminopyrine in rat hepatocytes according to the method described by Nash. Results obtained showed that aminopyrine N-demethylase activity measured from all diabetic rat hepatocytes was not affected by 0. stamineus and M. citrifolia extract. A significant decrease in the aminopyrine N-demethylase activity was observed in the normal old male SD rat hepatocytes preincubated with 0.1 mg/kg (P< 0.01 of methanol extract of 0. stamineus when compared to the respective control group. M. citrifolia juice extract at 0.1 mg/ml (P< 0.01 significantly increased aminopyrine N-demethylase activity in normal adult male SD rat hepatocytes as compared to the control group. For conclusion, both M. citrifolia and 0. stamineus extract could affect the in vitro metabolism of aminopyrine by CYP3A in normal rat hepatocytes. No significant change in the hepatic aminopyrine metabolism was observed in the diabetic rats after incubating with different concentrations of M. citrifolia and 0. stamineus extracts. The observed herb-drug interactions in the present study was age- and disease-dependent.

  9. Sphingomonas morindae sp. nov., isolated from Noni (Morinda citrifolia L.) branch.

    Science.gov (United States)

    Liu, Yang; Yao, Su; Lee, Yong-Jae; Cao, Yanhua; Zhai, Lei; Zhang, Xin; Su, Jiaojiao; Ge, Yuanyuan; Kim, Song-Gun; Cheng, Chi

    2015-09-01

    Two yellow bacterial strains, designated NBD5(T) and NBD8, isolated from Noni (Morinda citrifolia L.) branch were investigated using a polyphasic taxonomic approach. Cells were Gram-stain-negative, aerobic, non-spore-forming, non-motile and short rod-shaped. Phylogenetic analysis based on 16S rRNA gene sequences suggested that the strains were members of a novel species of the genus Sphingomonas, the seven closest neighbours being Sphingomonas oligoaromativorans SY-6(T) (96.9% similarity), Sphingomonas polyaromaticivorans B2-7(T) (95.8%), Sphingomonas yantingensis 1007(T) (94.9%), Sphingomonas sanguinis IFO 13937(T) (94.7%), Sphingomonas ginsenosidimutans Gsoil 1429(T) (94.6%), Sphingomonas wittichii RW1(T) (94.6%) and Sphingomonas formosensis CC-Nfb-2(T) (94.5%). Strains NBD5T and NBD8 had sphingoglycolipid, phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol and phosphatidylcholine as the major polar lipids, ubiquinone 10 as the predominant respiratory quinone, and sym-homospermidine as the major polyamine. Strains NBD5(T) and NBD8 were clearly distinguished from reference type strains based on phylogenetic analysis, DNA-DNA hybridization, fatty acid composition data analysis, and comparison of a range of physiological and biochemical characteristics. It is evident from the genotypic and phenotypic data that strains NBD5(T) and NBD8 represent a novel species of the genus Sphingomonas, for which the name Sphingomonas morindae sp. nov. is proposed. The type strain is NBD5(T) ( = DSM 29151(T) = KCTC 42183(T) = CICC 10879(T)).

  10. Morinda citrifolia (Noni: A comprehensive review on its industrial uses, pharmacological activities, and clinical trials

    Directory of Open Access Journals (Sweden)

    Reem Abou Assi

    2017-07-01

    Full Text Available Traditional medical practitioners in Hawaii and Polynesia have used Morinda citrifolia L. (Noni for centuries to cure or prevent varieties of illnesses. The popularity of M. citrifolia as a dietary supplement, a food functional ingredient, or as a natural health enhancer is increasing throughout the world. M. citrifolia contains phytochemicals that own antibacterial, antiviral, antifungal, antitumor, anthelminthic, analgesic, hypotensive, anti-inflammatory and immune enhancing effects. Moreover, the increasing vogue of M. citrifolia has attracted industries to employ it as a part of various products and for wide applications such as a natural source of medicines and chemical reagents as well as a green insecticidal. The wide spread of M. citrifolia in tropical climate of the globe, from USA to Brazil reaching to Tahiti, Malaysia and Australia, contributed in enriching its uses and potentials due to the variation in harvest locations. M. citrifolia parts including fruits, seeds, barks, leaves, and flowers are utilized on their own for individual nutritional and therapeutical values, however, the fruit is considered to contain the most valuable chemical compounds. This review discusses in details the industrial uses and the pharmacological activities of M. citrifolia fruit, seed, leaf and root, along with their isolated phytochemical compounds, through describing the conducted in vitro and in vivo studies as well as clinical data.

  11. Do the health claims made for Morinda citrifolia (Noni) harmonize with current scientific knowledge and evaluation of its biological effects.

    Science.gov (United States)

    Gupta, Rakesh Kumar; Patel, Amit Kumar

    2013-01-01

    Morinda citrifolia, also known as Great Morinda, Indian Mulberry, or Noni, is a plant belonging to the family Rubiaceae. A number of major chemical compounds have been identified in the leaves, roots, and fruits of Noni plant. The fruit juice is in high demand in alternative medicine for different kinds for illnesses such as arthritis, diabetes, high blood pressure, muscle ached and pains, menstrual difficulties, headache, heart diseases, AIDS, gastric ulcer, sprains, mental depression, senility, poor digestion, arteriosclerosis, blood vessel problems, and drug addiction. Several studies have also demonstrated the anti-inflammatory, antioxidant and apoptosis-inducing effect of Noni in various cancers. Based on a toxicological assessment, Noni juice was considered as safe. Though a large number of in vitro, and, to a certain extent, in vivo studies demonstrated a range of potentially beneficial effects, clinical data are essentially lacking. To what extent the findings from experimental pharmacological studies are of potential clinical relevance is not clear at present and this question needs to be explored in detail before an recommendations can be made.

  12. Effect of Morinda citrifolia fruit extract and its iridoid glycosides on blood fluidity.

    Science.gov (United States)

    Murata, Kazuya; Abe, Yumi; Futamura-Masuda, Megumi; Uwaya, Akemi; Isami, Fumiyuki; Deng, Shixin; Matsuda, Hideaki

    2014-07-01

    The aim of this study was to investigate the effect of Morinda citrifolia fruit on blood fluidity. M. citrifolia fruit extract (MCF-ext) was investigated for its influence on blood aggregation and fibrinolysis. MCF-ext inhibited polybrene-induced erythrocyte aggregation and thrombin activity. The fibrinolytic activity of MCF-ext, in the euglobulin lysis time test and fibrin plate assay, is reported here for the first time. One of the active compounds was an iridoid glycoside, asperulosidic acid. The results indicated that MCF-ext is a potentially useful health food which is capable of improving blood flow and preventing lifestyle-related diseases.

  13. Chemical components of the roots of Noni (Morinda citrifolia) and their cytotoxic effects.

    Science.gov (United States)

    Lv, Lishuang; Chen, Huadong; Ho, Chi-Tang; Sang, Shengmin

    2011-06-01

    Roots of Morinda citrifolia (Noni or Yor in Thai) have been used traditionally for thousands of years to treat chronic diseases such as cancer and heart disease. In this study, three new anthraquinones together with 15 known compounds were isolated from the roots of M. citrifolia (Rubiaceae). Their structures were established by spectroscopic methods, particularly 1D and 2D NMR techniques. Six known compounds, together with two new compounds (2 and 3) showed significant inhibitory effects on the proliferation of human lung and colon cancer cells.

  14. Morinda citrifolia L. leaf extract as antibacterial Salmonella typhimurium to increase productivity of quail (Coturnix coturnix japonica).

    Science.gov (United States)

    Retnani, Y; Dan, T M Wardiny; Taryati

    2014-04-01

    The objective of this study was to apply effect of Morinda citrifolia L. citrifolia L. leaf extract as antibacterial of Salmonella typhimurium on mortality of Day Old Quail (DOQ), egg production and Hen day, hatchability of layer quail. This research was conducted at Laboratory of microbiology and laboratory of poultry nutrition, faculty of animal science, bogor agricultural university and slamet quail farms cilangkap, sukabumi, west java, Indonesia on March-July 2012. Two hundred and forty heads of quail were randomly assigned to four dietary treatments (sixty heads of quail/treatment). Experimental design used was Completely Randomized Design (CRD). The treatments consist of level of biscuit Morinda citrifolia L. Citrifolia L. leaf extract i.e R1 = 0%, R2 = 5%, R3 = 10%, R4 = 15%. The results indicated the treatments had significant effect (p Quail (DOQ). The average mortality of Day Old Quail (DOQ) was given extract Morinda citrifolia L. leaf were R1 (4.00%), R2 (1.00%), R3 (1.33%), R4 (0.67%). The average mortality of Day Old Quail (DOQ) was given 15% extract Morinda citrifolia L. leaf (R4) was lowest than control treatment (R1). The results of the analysis indicated that Morinda citrifolia L. leaf of quail drink had not significant effect (p > 0.05) on egg production, hen day and hatchability. It was concluded that the Morinda citrifolia L. leaf extract 15% can reduce mortality of Day Old Quail (DOQ) and can increase its egg production, hen day and hatchability.

  15. Application of chromatography and mass spectrometry to the characterization of cobalt, copper, manganese and molybdenum in Morinda citrifolia.

    Science.gov (United States)

    Rybak, Justyna; Ruzik, Lena

    2013-03-15

    An analytical procedure was proposed to determine the manganese species and to study the fractionation of microelements such as copper, cobalt and molybdenum in Noni juice. Morinda citrifolia is known as a noni fruit, Indian mulberry, nunaakai, dog dumpling, mengkudu, beach mulberry, vomit fruit and cheese fruit. It is a tropical plant with a long tradition of medicinal use in Polynesia and tropical parts of eastern Asia and Australia. This article covers the determination of manganese species in Noni juice and established by fractionation by size exclusion chromatography inductively coupled plasma mass spectrometry (SEC ICP MS) and next characterization of species by electrospray ionization mass spectrometry (ESI MS). Also presented the fractionation analysis of copper, cobalt and molybdenum in Noni juice sample using SEC ICP MS - juice was treated with buffer and enzymatic extraction media and analyzed. For the evaluation of the amounts of the metal fractions distinguished, the ICP MS was used off-line prior to the determination of copper, cobalt, molybdenum and manganese concentrations in the juice. It was established that elements are present in the analyzed samples in different species and their concentration is μg mL(-1) and ng mL(-1) range in fruit. The accuracy of the entire fractionation scheme and sample preparation procedures involved was verified by the performance of the recovery test. For the information about the bioavailability of these elements, in vitro bioavailability investigation was used by SEC ICP MS technique. Two step digestion model simulating gastric (pepsin digestion) and intestinal (pancreatin digestion) juices. In Noni juice, manganese is complexed from flavonoids - rutin, from dye like anthraquinone (alizarin) and glycosides - asperulosidic acid (ESI MS - characterization). The study shows that copper and molybdenum contained in Noni juice are complexed by peptides, and cobalt by organic acids (which are 3.6% of juice). Molybdenum in

  16. Antipsychotic-like activity of Noni (Morinda citrifolia Linn.) in mice

    OpenAIRE

    Pandy Vijayapandi; Narasingam Megala; Mohamed Zahurin

    2012-01-01

    Abstract Background Noni fruit is widely consumed in tropical regions of Indonesia to the Hawaiian Islands. The noni plant has a long history of use as a medicinal plant to treat a wide variety of ailments including CNS disorders. The present investigation was designed to evaluate the antipsychotic effect of noni fruits (Morinda citrifolia Linn.) using mouse models of apomorphine-induced climbing behaviour and methamphetamine-induced stereotypy (licking, biting, gnawing and sniffing). Methods...

  17. Morinda citrifolia (Noni): A literature review and recent advances in Noni research

    Institute of Scientific and Technical Information of China (English)

    WANG Mian-Ying; Brett J WEST; C Jarakae JENSEN; Diane NOWICKI; SU Chen; Afa K PALU; Gary ANDERSON

    2002-01-01

    Morinda citrifolia L (Noni) has been used in folk remedies by Polynesians for over 2000 years, and is reported to have a broad range of therapeutic effects, including antibacterial, antiviral, antifungal, antitumor, antihelmin,analgesic, hypotensive, anti-inflammatory, and immune enhancing effects. In order to reveal the nutritional and medicinal value of the Noni plant, and to summarize scientific evidence that supports the Polynesians' claim, a literature review and recent advances in Noni research is given below.

  18. Optimisation of spray drying operating conditions of Morinda citrifolia L. fruit extract using response surface methodology

    OpenAIRE

    2015-01-01

    A conventional solvent extract of Morinda citrifolia L. fruit was spray dried using adjuvant maltodextrin (5 wt.%). Spray drying was carried out according to the D-optimal design, and the independent variables selected were temperature and Mcore/Mwall. The spray drying process was optimised by using response surface methodology (RSM) for four different responses: moisture content (MC), DPPH scavenging activity, total phenolic content (TPC), and total flavonoid (TF). The effects of temperature...

  19. Performances of broiler supplemented with antibiotic and Morinda citrifolia waste as sources of bioactive compounds

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    I.A.K Bintang

    2008-03-01

    Full Text Available A study on the use of Zinc bacitracin antibiotic (ZnB and dried Morinda citrifolia waste as feed additive in broiler ration was conducted. Two hundred and sixteen day old chicks (DOC was allocated into 4 treatments: control; control + 50 ppm ZnB, and control + M. citrifolia waste at 2 levels (5 and 10 g/kg ration with 9 replications. Each replicatation consist of 6 birds. The treatments were allocated in a completely randomized design. Variables measured were feed intake, live weight, feed conversion ratio (FCR, percentages of carcass and internal organs (liver, gizard, abdominal fat, and thickness of intestine. The results showed that feed intake of M. citrifolia waste was significantly (P0.05 from that of control. The use of M. citrifolia waste (5 and 10 g/kg gave significantly lower (P0.05, but was significantly lower (P0,05 than that of control, control + antibiotic ZnB and M. citrifolia waste 5g/kg. It is concluded that the supplementation of M. citrifolia waste (5 g/kg could substitute ZnB to improve feed eficiency in broiler ration.

  20. PENGUKURAN KANDUNGAN SKOPOLETIN PADA BEBERAPA TINGKAT KEMATANGAN BUAH MENGKUDU (Morinda citrifolia Linn DENGAN METODE KLT DENSITOMETRI

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    Diana Nurus Sholehah

    2010-03-01

    Full Text Available Morinda citrifolia fruit has been used as remedies in Indonesia because many diseases could be healed. Scopoletin, one of major component of Morinda citrifolia was chosen as  marker compound because of  its various activites such as  hypotensive, antiinflamatory and antihistamine. The research aimed to determinate scopoletin in some maturations stage of Morinda citrifolia fruit in order to get best fruit for traditional medicine and phytopharmaca with optimal therapeutical effect . A simple and reliable TLC-Densitometry which has been validated before, was choosen to this aim. Fruit at stage 1,2,3 and 4 were taken  20, 45, 105 and 120 days after flowering. TLC was held on silica gel plates as stationary and ether : toluene : acetic acid 10 % (58:45:0,4 as mobile phase of fruit at stage 1, 2 and 4 and also ether : toluene : acetic acid 10 % (58:45:0,8 as mobile phase of fruit at stage 3.  Mean concentration of scopoletin at stage 1 was found to be (10,72±0,45 ppm, stage 2 was found to be (19,19±0,68 ppm, stage 3 found to be (57,94±0,79 ppm and stage 4 (14,11±0,39 ppm respectively.

  1. Mechanical scarification in seeds of Morinda citrifolia aiming to speed up the process of germination

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    M. J. C. Gomes

    2013-07-01

    Full Text Available Abstract: Morinda Citrifolia are plants known as important properties with pharmacological and medicine, and the growth of this kind of plant has been increased in the last years. This work has as objective of developing different methods of seed germination of the Morinda citrifolia. The work was carried out in the laboratory of seed analyses at federal university of mato grosso, Sinop. The seeds were collected in plants of Bianchi farm. The material was benefited in the shade and stored in a container by 30 days. Used DIC as design, with treatment and 4 reprises; T1 witness; T2 with smashed seeds part; T3 seeds in water for 6 hours and T4 with smashed seeds part in water for 6 hours. For each treatment was studied with 4 reprises of 25 seeds, The seeds were treated with hypochlorite in 2%. The germination was conducted in special papers of germination, in greenhouse BOD at 30C. The tests of rate germination and speed was started in 24 hours after the installation of experiment. T1 and T3 doesnt present germination, the treatment T4 and presents germination between 42 % and 30% respective. T4 presented IUV of 1,77 and T2 of 2,77, been this treatments recommended for germination of Morinda citrifolia.Key words: Germination, numb, seeds.

  2. Anti-angiogenic activity of Morinda citrifolia extracts and its chemical constituents.

    Science.gov (United States)

    Beh, Hooi-Kheng; Seow, Lay-Jing; Asmawi, Mohd Zaini; Abdul Majid, Amin Malik Shah; Murugaiyah, Vikneswaran; Ismail, Norhayati; Ismail, Zhari

    2012-01-01

    Morinda citrifolia L. has been used for the treatment of a wide variety of diseases, including cancer. This study was undertaken to evaluate the anti-angiogenic effect of M. citrifolia fruits and leaves. Anti-angiogenic activity was evaluated in vivo using the chick chorioallantoic membrane assay. Bioactivity-guided fractionation and isolation were performed to identify the active constituent, and high-performance liquid chromatography analysis was then used to quantify the amount of this active constituent in the active extracts and fraction. The methanol extracts of fruits and leaves of M. citrifolia and the subsequent chloroform fraction of the fruit methanolic extract were found to have potential anti-angiogenic activity and were more potent compared to suramin. Scopoletin was identified as one of the chemical constituents that may be partly responsible for the anti-angiogenic activity of M. citrifolia fruits. The present findings further support the use of M. citrifolia in cancer or other pathological conditions related to angiogenesis.

  3. Supplementation of Morinda citrifolia waste as bioactive compound on the performances of broiler

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    I.A.K Bintang

    2007-03-01

    Full Text Available A study on the use of dried Morinda citrifolia waste as feed additive in broiler ration was conducted. One hundred day old chicks (doc were allocated into 4 levels (control; 0.0; 1.2; 2.4 and 4.8 g/kg feed of M. citrifolia waste with 5 replication. Each replication had 5 birds. The treatments were allocated in a completely randomized design. Variables measured were: feed intake, live weight, feed conversion ratio (FCR, percentages of carcass and internal organs ( liver. gizard, abdominal fat, and thickness of intestine. The results showed that M citrifolia waste supply did not significantly (P>0.05 affect all variables measured, but feed intake of those fed with high levels (4,8 g/kg of M. citrifolia waste was significantly (P<0.05 lower than those fed with 1.2 g/kg. FCR was significantly (P<0.05 lower as compared to the control; 1.2 and 2.4 g/kg. It is concluded that the best treatment was the supplementation of M. citrifolia waste of 4.8 g/kg, this treatment improved feed efficiency by 5% as compared to the control.

  4. Effect of dietary supplementation with Morinda citrifolia on productivity and egg quality of laying hens

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    Dairon Más-Toro

    2015-07-01

    Full Text Available In order to evaluate the dietary supplementation of powdered leaves of Morinda citrifolia on productivity and egg quality of laying hens, a total of 160 White Leghorn birds (Hybrid L-33 of 27 weeks of age were allotted during 70 days, according to completely randomized design. Dietary treatments consisted of a control diet fed without or with 0.5, 1.0 and 1.5% of powdered leaves of M. citrifolia. Supplementation of 1.0 and 1.5% of M. citrifolia powder increased the egg weight (P0.05 among treatments. Also, supplementation of 0.5 and 1.0% of M. citrifolia increased the shell thickness and the yolk color was pigmented by this medicinal plant. It recommended the dietary supplementation of 1.0% of powdered leaves of M. citrifolia on laying hen diets to improve the egg weight, shell thickness and yolk color.

  5. Breast cancer prevention with Morinda citrifolia (noni at the initiation stage

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    Mian-Ying Wang

    2013-06-01

    significantly increased compared with positive controls at different time points. Histological examination showed that the malignancy of lesions in TNJ groups did not show a significant change when compared with that in positive and placebo groups.Conclusion: In conclusion, this is the first study which indicates that TNJ possesses a cancer preventive effect at the initiation stage of chemical carcinogenesis induced by DMBA in female SD rates.Key words: Morinda citrifolia, Tahitian noni® juice, breast cancer, cancer prevention

  6. UVA-UVB Photoprotective Activity of Topical Formulations Containing Morinda citrifolia Extract

    Science.gov (United States)

    Serafini, Mairim Russo; Detoni, Cassia Britto; Menezes, Paula dos Passos; Pereira Filho, Rose Nely; Fortes, Vanessa Silveira; Vieira, Maria José Fonseca; Guterres, Sílvia Stanisçuaski; de Albuquerque Junior, Ricardo Luiz Cavalcanti; Araújo, Adriano Antunes de Souza

    2014-01-01

    Exposure to solar radiation, particularly its ultraviolet (UV) component, has a variety of harmful effects on human health. Some of these effects include sunburn cell formations, basal and squamous cell cancers, melanoma, cataracts, photoaging of the skin, and immune suppression. The beneficial photoprotective effects of topical formulations with the extract, Morinda citrifolia, have not been investigated. This present study aims to investigate the potential benefits of M. citrifolia topical application on the dorsal skin of mice, exposed to UVA-UVB light. Using 7 days of treatment, [before (baseline values) and 20 h after UV exposure], the thickness, skin barrier damage (TEWL), erythema, and histological alterations were evaluated. The results showed that the formulations containing the extract protected the skin against UV-induced damage. PMID:25133171

  7. UVA-UVB photoprotective activity of topical formulations containing Morinda citrifolia extract.

    Science.gov (United States)

    Serafini, Mairim Russo; Detoni, Cassia Britto; Menezes, Paula dos Passos; Pereira Filho, Rose Nely; Fortes, Vanessa Silveira; Vieira, Maria José Fonseca; Guterres, Sílvia Stanisçuaski; Cavalcanti de Albuquerque Junior, Ricardo Luiz; Araújo, Adriano Antunes de Souza

    2014-01-01

    Exposure to solar radiation, particularly its ultraviolet (UV) component, has a variety of harmful effects on human health. Some of these effects include sunburn cell formations, basal and squamous cell cancers, melanoma, cataracts, photoaging of the skin, and immune suppression. The beneficial photoprotective effects of topical formulations with the extract, Morinda citrifolia, have not been investigated. This present study aims to investigate the potential benefits of M. citrifolia topical application on the dorsal skin of mice, exposed to UVA-UVB light. Using 7 days of treatment, [before (baseline values) and 20 h after UV exposure], the thickness, skin barrier damage (TEWL), erythema, and histological alterations were evaluated. The results showed that the formulations containing the extract protected the skin against UV-induced damage.

  8. Inhibitory effects of constituents of Morinda citrifolia seeds on elastase and tyrosinase.

    Science.gov (United States)

    Masuda, Megumi; Murata, Kazuya; Fukuhama, Akiko; Naruto, Shunsuke; Fujita, Tadashi; Uwaya, Akemi; Isami, Fumiyuki; Matsuda, Hideaki

    2009-07-01

    A 50% ethanolic extract (MCS-ext) from seeds of Morinda citrifolia ("noni" seeds) showed more potent in vitro inhibition of elastase and tyrosinase, and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity than extracts of M. citrifolia leaves or flesh. Activity-guided fractionation of MCS-ext using in vitro assays led to the isolation of ursolic acid as an active constituent of elastase inhibitory activity. 3,3'-Bisdemethylpinoresinol, americanin A, and quercetin were isolated as active constituents having both tyrosinase inhibitory and radical scavenging activities. Americanin A and quercetin also showed superoxide dismutase (SOD)-like activity. These active compounds were isolated from noni seeds for the first time.

  9. Immunostimulant activity of noni (Morinda citrifolia) on T and B lymphocytes.

    Science.gov (United States)

    Nayak, Smita; Mengi, Sushma

    2010-07-01

    Morinda citrifolia Linn (Rubiaceae) is a traditional medicinal herb that has been purported to be beneficial in the treatment of infections due to its immune enhancing properties. However, detailed studies highlighting the effect of different compounds isolated from the plant on the immune system are lacking. In this study, the stimulatory effects of the extracts and fractions of M. citrifolia fruits on important components of the adaptive immune system such as T lymphocytes and B lymphocytes were studied. The effects of the plant extracts on lymphocytes were assessed by in vitro (MTT assay) and in vivo (cell mediated immune response) techniques. Results of the MTT study indicated that the hydroalcoholic (0.5 and 1.0 mg/mL) and aqueous extracts (0.5 and 1.0 mg/mL) significantly (p citrifolia fruits on B-cells was measured by the delayed type hypersensitivity method. The study revealed that the hydroalcoholic extract (200 mg/kg) and fraction F I (40 mg/kg) significantly increased the humoral response to the extent of 33.33 and 35.12%, respectively. The results of this study confirm the cellular and humoral immunostimulant properties of M. citrifolia fruits and justify its usage in traditional medicine.

  10. Antidiabetic Effect of Morinda citrifolia (Noni Fermented by Cheonggukjang in KK-Ay Diabetic Mice

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    So-Young Lee

    2012-01-01

    Full Text Available Antidiabetic effects of Morinda citrifolia (aka Noni fermented by Cheonggukjang (fast-fermented soybean paste were evaluated using a T2DM (type 2 diabetes mellitus murine model. Six-week-old KK-Ay/TaJcl mice were randomly divided into four groups: (1 the diabetic control (DC group, provided with a normal mouse diet; (2 the positive control (PC group, provided with a functional health food diet; (3 the M. citrifolia (MC group, provided with an MC-based diet; (4 the fermented M. citrifolia (FMC group, provided with an FMC-based diet. Over a testing period of 90 days, food and water intake decreased significantly in the FMC and PC groups compared with the DC group. Blood glucose levels in the FMC group were 211.60–252.20 mg/dL after 90 days, while those in the control group were over 400 mg/dL after 20 days. In addition, FMC supplementation reduced glycosylated hemoglobin (HbA1c levels, enhanced insulin sensitivity, and significantly decreased serum triglycerides and low-density lipoprotein (LDL cholesterol. Furthermore, a fermented M. citrifolia 70% ethanolic extract (FMCE activated peroxisome proliferator-activated receptor-(PPAR- γ and stimulated glucose uptake via stimulation of AMP-activated protein kinase (AMPK in cultured C2C12 cells. These results suggest that FMC can be employed as a functional health food for T2DM management.

  11. Activation of cell-mediated immunity by Morinda citrifolia fruit extract and its constituents.

    Science.gov (United States)

    Murata, Kazuya; Abe, Yumi; Futamura-Masudaa, Megumi; Uwaya, Akemi; Isami, Fumiyuki; Matsuda, Hideaki

    2014-04-01

    Morinda citrifolia, commonly known as noni, is a traditional natural medicine in French Polynesia and Hawaii. Functional foods derived from M. citrifolia fruit have been marketed to help prevent diseases and promote good health. The objective of this study was to assess the effects of M. citrifolia fruit on cell-mediated immunity. In the picryl chloride-induced contact dermatitis test, M. citrifolia fruit extract (Noni-ext) inhibited the suppression of cell-mediated immunity by immunosuppressive substances isolated from freeze-dried ascites of Ehrlich carcinoma-bearing mice (EC-sup). In addition, Noni-ext inhibited reduction of IL-2 production in EC-sup-treated mice and activated natural killer cells in normal mice. These results suggest that Noni-ext has multiple effects on the recovery of cell-mediated immunity. Furthermore, we investigated the active principles of Noni-ext and identified an iridoid glycoside, deacetylasperulosidic acid. Oral administration of deacetylasperulosidic acid inhibited the reduction of ear swelling, and also cancelled the suppression of IL-2 production along with the activation of natural killer cells in the same manner as that of Noni-ext.

  12. Antidiabetic Effect of Morinda citrifolia (Noni) Fermented by Cheonggukjang in KK-A(y) Diabetic Mice.

    Science.gov (United States)

    Lee, So-Young; Park, So-Lim; Hwang, Jin-Taek; Yi, Sung-Hun; Nam, Young-Do; Lim, Seong-Il

    2012-01-01

    Antidiabetic effects of Morinda citrifolia (aka Noni) fermented by Cheonggukjang (fast-fermented soybean paste) were evaluated using a T2DM (type 2 diabetes mellitus) murine model. Six-week-old KK-Ay/TaJcl mice were randomly divided into four groups: (1) the diabetic control (DC) group, provided with a normal mouse diet; (2) the positive control (PC) group, provided with a functional health food diet; (3) the M. citrifolia (MC) group, provided with an MC-based diet; (4) the fermented M. citrifolia (FMC) group, provided with an FMC-based diet. Over a testing period of 90 days, food and water intake decreased significantly in the FMC and PC groups compared with the DC group. Blood glucose levels in the FMC group were 211.60-252.20 mg/dL after 90 days, while those in the control group were over 400 mg/dL after 20 days. In addition, FMC supplementation reduced glycosylated hemoglobin (HbA1c) levels, enhanced insulin sensitivity, and significantly decreased serum triglycerides and low-density lipoprotein (LDL) cholesterol. Furthermore, a fermented M. citrifolia 70% ethanolic extract (FMCE) activated peroxisome proliferator-activated receptor-(PPAR-) γ and stimulated glucose uptake via stimulation of AMP-activated protein kinase (AMPK) in cultured C2C12 cells. These results suggest that FMC can be employed as a functional health food for T2DM management.

  13. ANTIOXIDANT ACTIVITY, TOTAL PHENOLIC AND FLAVONOID CONTENT OF MORINDA CITRIFOLIA FRUIT EXTRACTS FROM VARIOUS EXTRACTION PROCESSES

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    PRAVEEN K. RAMAMOORTHY

    2007-04-01

    Full Text Available Soxhlet, Ultrasonic extract of Morinda citrifolia L. fruit and four extracts from high pressure extraction at 10 MPa using ethanol, ethyl acetate as solvent and dried by vacuum oven and spray dryer were analyzed for their antioxidant activity by peroxide value method and diphenylpicrylhydrazyl radical scavenging method. The five extracts along with the reference samples, butylated hydroxyl toluene and tannic acid were further analyzed to determine their total phenolic content by Folin-Ciocalteau method and total flavonoid content by Dowd method. The M. citrifolia extract by high pressure extraction with ethyl acetate as solvent and spray dried was found to exhibit highest antioxidant activity and total flavonoid content. High total phenolic content was determined in the high pressure extract using ethyl acetate as solvent and vacuum dried. It was interesting to note that ultrasonic extract exhibited significant antioxidant activity, total phenolic and flavonoid content. High pressure extracted M. citrifolia in ethanol was found to express lesser values comparatively. The significant difference in activity among the high pressure extracts was found to be due to the polarity of the solvents used for extraction as M. citrifolia fruit contains relatively larger quantity of non-polar antioxidant compounds. It was also found that the drying methods had significant impact on the antioxidant activity, total phenolic and flavonoid content of the extracts.

  14. Antitubercular constituents from the hexane fraction of Morinda citrifolia Linn. (Rubiaceae).

    Science.gov (United States)

    Saludes, Jonel P; Garson, Mary J; Franzblau, Scott G; Aguinaldo, Alicia M

    2002-11-01

    A crude ethanol extract and hexane fraction from Morinda citrifolia Linn. (Rubiaceae) show antitubercular activity. The major constituents of the hexane fraction are E-phytol, cycloartenol, stigmasterol, beta-sitosterol, campesta-5,7,22-trien-3beta-ol and the ketosteroids stigmasta-4-en-3-one and stigmasta-4-22-dien-3-one. E-Phytol, a mixture of the two ketosteroids, and the epidioxysterol derived from campesta-5,7,22-trien-3beta-ol all show pronounced antitubercular activity.

  15. Morinda citrifolia linn leaf extract possesses antioxidant activities and reduces nociceptive behavior and leukocyte migration

    OpenAIRE

    Serafini, Mairim Russo; Santos, Rodrigo Correia; Guimarães, Adriana Gibara; Santos, João Paulo Almeida dos; Santos, Alan Diego da Conceição; Alves, Izabel Almeida; Gelain, Daniel Pens; Nogueira, Paulo Cesar de Lima; Quintans-Júnior, Lucindo José; Bonjardim, Leonardo Rigoldi; Araújo, Adriano Antunes de Souza

    2011-01-01

    This is a copy of an article published in the Journal of Medicinal Food© 2011 - copyright Mary Ann Liebert, Inc.; Journal of Medicinal Food is available online at: http://online.liebertpub.com Herbal drugs have been used since ancient times to treat a wide range of diseases. Morinda citrifolia Linn (popularly known as ‘‘Noni’’) has been used in folk medicine by Polynesians for over 2,000 years. It is reported to have a broad range of therapeutic effects, including effects against headache,...

  16. Isolation and structure determination of two new constituents from the fruits of Morinda citrifolia Linn.

    Science.gov (United States)

    Siddiqui, Bina S; Sattar, Fouzia A; Ahmad, Fayaz; Begum, Sabira

    2008-01-01

    Studies on the chemical constituents of the fruits of Morinda citrifolia Linn. have led to the isolation of two new compounds, morinaphthalene (=1,3,6,7-tetrahydroxy-2-hydroxymethyl-1,2,3,4-tetrahydronaphthalene, (1); and morindafurone (=5-hydroxy-1,10b-dihydro-6H-anthra [1,9-bc] furan-6-one, (2); as well as two known constituents, 1,8-dihydroxy-6-methoxy-3-methyl-9-anthrone (3) and 2,4-dimethoxy-9-anthrone (4). Their structures were elucidated by spectral analysis including 2D NMR techniques.

  17. Phyto chemical Screening, Antibacterial, Antifungal and Anthelmintic Activity of Morinda citrifolia stem

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    Dr. D. Gopala Krishna

    2013-05-01

    Full Text Available In the present study, the Petroleum Ether and Alcoholic extract of Morinda citrifolia L. (Noni stem were subjected to preliminary screening for Antimicrobial and Aanthelmintic activity. The alcoholic extract exhibited significant Anti bacterial, Antifungal activity, comparable to the standard drug Tetracycline. The Petroleum Ether and Alcoholic extract were evaluated for Anthelmintic activity on adult Indian Earthworms, ‘Pheretima posithuma’. The Alcoholic extract produced more significant Anthelmintic activity than Petroleum ether extract and the activities are comparable with the reference drug Piperazine citrate

  18. Anti-jugular vein thrombotic effect of Morinda citrifolia L. [noni] in male SD rats

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    Mian-Ying Wang

    2011-09-01

    Full Text Available Background: Venous thromboembolism (VTE is a common and serious medical condition, which is estimably responsible for more than 300,000 hospital admissions annually in the USA. Pulmonary embolism (PE is a major complication of VTE, which contributes to 12% death of hospitalized patients. Heparin is the most common anti-coagulant, but severe allergic reactions, bleeding, and thrombocytopenia limit its use. Thus, seeking a botanical, nontoxic antithrombotic alternative is an interesting area. Morinda citrifolia L. [noni] is a medicinal plant used in folk remedies by Polynesians for over 2,000 years. It has been reported to have a broad range of therapeutic and preventive effects. The bioactivities of NJ have been continuously discovered with antioxidative, anti-inflammatory, analgesic, and immune modifying activities. Our novel hypothesis is whether NJ has an anti-venous thrombotic effect in rodents. To examine our hypothesis, this study was designed to examine the anti-thrombotic effect of NJ on the jugular vein thrombosis model induced by ferric chloride in SD rats.Material and Methods: NJ and placebo used in this study were donated by Morinda Holding Inc. NJ was formulated with grape juice and blue berry juice. Placebo was prepared by using the same procedure of NJ preparation, but without NJ in it. Thirty-six male SD rats were divided into six groups. Anti-venous thrombotic activities of 5% NJ, 10% NJ, heparin, and 10% NJ plus heparin were examined and compared with the positive and blank controls. Thrombosis was induced by application of a filter paper soaked in 50% ferric chloride on the right jugular vein. AFunctional Foods in Health and Disease 2011; 9:297-3092-cm fragment of the occluded vein (thrombus was removed and weighed after 1-hour maturation. Blood samples were collected for platelet count, aPTT, and PT tests.Results: The weight of a 2-cm fragment of normal jugular vein was 9.9 ± 2.1 mg, while the weight of the occluded vein in

  19. Studies on antidyslipidemic effects of Morinda citrifolia (Noni fruit, leaves and root extracts

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    Mandukhail Saf-ur

    2010-08-01

    Full Text Available Abstract Background The objective of present study was to provide the pharmacological basis for the medicinal use of Morinda citrifolia Linn in dyslipidemia using the aqueous-ethanolic extracts of its fruits (Mc.Cr.F, leaves (Mc.Cr.L and roots (Mc.Cr.R. Results Mc.Cr.F, Mc.Cr.L and Mc.Cr.R showed antidyslipidemic effects in both triton (WR-1339 and high fat diet-induced dyslipidemic rat models to variable extents. All three extracts caused reduction in total cholesterol and triglyceride levels in triton-induced dyslipidemia. In high fat diet-induced dyslipidemia all these extracts caused significant reduction in total cholesterol, triglyceride, low density lipoprotein-cholesterol (LDL-C, atherogenic index and TC/HDL ratio. Mc.Cr.R extract also caused increase in high density lipoprotein-cholesterol (HDL-C. The Mc.Cr.L and Mc.Cr.R reduced gain in body weight with a reduction in daily diet consumption but Mc.Cr.F had no effect on body weight and daily diet consumption. Conclusions These data indicate that the antidyslipidemic effect of the plant extracts was meditated through the inhibition of biosynthesis, absorption and secretion of lipids. This may be possibly due partly to the presence of antioxidant constituents in this plant. Therefore, this study rationalizes the medicinal use of Morinda citrifolia in dyslipidemia.

  20. ENVIRONMENTAL SCANNING ELECTRON MICROSCOPY (ESEM OF Morinda citrifolia L. (Rubiaceae COLLETERS

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    Sergimar Kennedy de Paiva PINHEIRO

    2015-12-01

    Full Text Available This study describes the colleters of Morinda citrifolia L. (Rubiaceae by using environmental scanning electron microscopy (ESEM and energy dispersive x-ray spectroscopy (EDS. Two different developmental stages were characterized as well as the chemical composition of secretion. Colleters are secretory structures that produce mucilage protecting the meristems and leaf primordia against desiccation and/or pathogens. Although these secretory structures are common on Rubiaceae, the results reported here is to the best of our knowledge the first record of colleters on Morinda genus. Colleters are present at the stipule adaxial surface, distributed in lines. These secretory structures are standard type and have no base constriction, differently from all studied species until now. In order to better understand the colleters structure and secretion, two phases were distinguished: a secretory phase and a senescence one. On secretory phase standard type colleters were visualized between leaf primordia and stipule, emerge on secretion. They present smooth surface, however was also possible to observe the contours of secretory cells anticlinal walls. In senescent phase colleters morphology was alternated exhibiting rough surface and blunt to point tips. The surface was rough and on stipule sections was possible to observe idioblasts with raphides bundles. The secretion process of M. citrifolia colleters occurs with the disruption of cuticle and the chemical elements are mostly dominated by carbon and oxygen.

  1. Morinda citrifolia L. (noni Improves the Quality of Life (QoL in Adults with Osteoarthritis

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    Martin S. Lipsky

    2011-02-01

    Full Text Available Background: Morinda citrifolia Linn (noni, as a “pain killer”, has been used as a traditional medicine by Polynesians for over 2000 years. It was reported to have a broad range of therapeutic effects including analgesic and anti-inflammation. The in-vitro and in vivo antiinflammatory and analgesic properties of noni juice (NJ suggest that NJ may be a useful adjunctive treatment for osteoarthritis (OA. In this pilot study we explored whether NJimproves the symptoms and Quality of Life (QoL for adults with OA. We also sought toevaluate the tolerability and safety of NJ for patients with OA in a primary care setting. Methods: This was an open label three-month intervention pilot study. Data were collected by pre/post intervention survey and laboratory testing. Inclusion criteria were: adults of both sexes aged 40 to 75, with a diagnosis of OA on the hip or knee by x-ray examination provided by theirprimary care physician, not on prescription medicine for OA, and who were willing to drink 3 oz of NJ a day for 90 days. Results: Of the 64 questions measuring different aspects of QoL asked on the pre/post survey, 49 (77% had significant pre/post mean scale differences as measured by independent t-test. The OA patients reported being significantly more satisfied with their current health conditions including mobility, walking and bending, hand, finger, and arm functions, household tasks, social activity, arthritis pain, work ability, level of tension, and mood. The study participantswere also more positive about their future health and reported taking less over-the-counter (OTC pain relievers. Pre/post laboratory testing including: lipid panel, liver and kidney functions were in the normal ranges. High Sensitive C Reactive Protein (hsCRP, an inflammatory biomarker, was reduced by 10% after the intervention. Conclusion: As a nutritional supplement, NJ demonstrated a potential therapeutic effect and improved the symptoms and the QoL for adults with OA

  2. Morinda citrifolia Linn leaf extract possesses antioxidant activities and reduces nociceptive behavior and leukocyte migration.

    Science.gov (United States)

    Serafini, Mairim Russo; Santos, Rodrigo Correia; Guimarães, Adriana Gibara; Dos Santos, João Paulo Almeida; da Conceicão Santos, Alan Diego; Alves, Izabel Almeida; Gelain, Daniel Pens; de Lima Nogueira, Paulo Cesar; Quintans-Júnior, Lucindo José; Bonjardim, Leonardo Rigoldi; de Souza Araújo, Adriano Antunes

    2011-10-01

    Herbal drugs have been used since ancient times to treat a wide range of diseases. Morinda citrifolia Linn (popularly known as "Noni") has been used in folk medicine by Polynesians for over 2,000 years. It is reported to have a broad range of therapeutic effects, including effects against headache, fever, arthritis, gingivitis, respiratory disorders, infections, tuberculosis, and diabetes. The aim of this study was to investigate the antioxidant, anti-inflammatory, antinociceptive, and antibacterial properties of the aqueous extract from M. citrifolia leaves (AEMC). Antioxidant activity was observed against lipid peroxidation, nitric oxide, and hydroxyl radicals. The antinociceptive effect of AEMC was observed in the acetic acid-induced writhing test at the higher dose. Moreover, AEMC significantly reduced the leukocyte migration in doses of 200 and 400 mg/kg and showed mild antibacterial activity. Together, the results suggest that properties of M. citrifolia leaf extract should be explored further in order to achieve newer tools for managing painful and inflammation conditions, including those related to oxidant states.

  3. Optimal Binary Solvent Extraction System for Phenolic Antioxidants from Mengkudu (Morinda citrifolia Fruit

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    Chin Ping Tan

    2013-06-01

    Full Text Available Antioxidants have been widely used in the food industry to enhance product quality by preventing oxidation of susceptible substances. This work was carried out to maximise the recovery of total phenolic content (TPC, total flavonoid content (TFC, 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS radical-scavenging capacity and 2,2′-diphenyl-1-picrylhydrazyl (DPPH radical-scavenging capacity from Morinda citrifolia fruit via modification of the ethanol concentration, extraction time and extraction temperature at minimal processing cost. The optimised conditions yielded values of 881.57 ± 17.74 mg GAE/100 g DW for TPC, 552.53 ± 34.16 mg CE/100 g DW for TFC, 799.20 ± 2.97 µmol TEAC/100 g DW for ABTS and 2,317.01 ± 18.13 µmol TEAC/100 g DW for DPPH were 75% ethanol, 40 min of time and 57 °C. The four responses did not differ significantly (p > 0.05 from predicted values, indicating that models obtained are suitable to the optimisation of extraction conditions for phenolics from M. citrifolia. The relative amounts of flavonoids were 0.784 ± 0.01 mg quercetin/g of extract and 1.021 ± 0.04 mg rutin/g of extract. On the basis of the results obtained, M. citrifolia extract can be used as a valuable bioactive source of natural antioxidants.

  4. Optimal binary solvent extraction system for phenolic antioxidants from mengkudu (Morinda citrifolia) fruit.

    Science.gov (United States)

    Thoo, Yin Yin; Ho, Swee Kheng; Abas, Faridah; Lai, Oi Ming; Ho, Chun Wai; Tan, Chin Ping

    2013-06-14

    Antioxidants have been widely used in the food industry to enhance product quality by preventing oxidation of susceptible substances. This work was carried out to maximise the recovery of total phenolic content (TPC), total flavonoid content (TFC), 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radical-scavenging capacity and 2,2'-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging capacity from Morinda citrifolia fruit via modification of the ethanol concentration, extraction time and extraction temperature at minimal processing cost. The optimised conditions yielded values of 881.57 ± 17.74 mg GAE/100 g DW for TPC, 552.53 ± 34.16 mg CE/100 g DW for TFC, 799.20 ± 2.97 µmol TEAC/100 g DW for ABTS and 2,317.01 ± 18.13 µmol TEAC/100 g DW for DPPH were 75% ethanol, 40 min of time and 57 °C. The four responses did not differ significantly (p > 0.05) from predicted values, indicating that models obtained are suitable to the optimisation of extraction conditions for phenolics from M. citrifolia. The relative amounts of flavonoids were 0.784 ± 0.01 mg quercetin/g of extract and 1.021 ± 0.04 mg rutin/g of extract. On the basis of the results obtained, M. citrifolia extract can be used as a valuable bioactive source of natural antioxidants.

  5. Buah Mengkudu (Morinda citrifolia Meningkatkan Respon Imun Mencit (Mus musculus Terhadap Infeksi Bakteri Staphylococcus aureus

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    Mufidah Zumrotul

    2015-11-01

    Full Text Available Noni (Morinda citrifolia Increase Immune Response in Mice (Mus musculus Infected Staphylococcus aureus. Infection disease caused by bacteria is one of the illness in several developing countries including in Indonesia, with high mortality rate. Infection of  S. aureus as the cause of problem resistance for antibiotic or multi drug resistance are giving the therapy of drug itself with change to medical herbal. The aim of this study is known the role of M.citrifolia extract to increase immune response of mice with infectioned of S. aureus. Mice were divided into two groups there are Non Infection and infection. Non Infection is without S. aureus and than infection has S. aureus. The each groups are including control, dose 1 (25 mg/kg BW, dose 2 (100 mg/kg BW, and dose 3 (300 mg/kg BW. Relative number of lymphocyte T cell (CD4+ subsets was measured  using the BD FACSCaliburTM Flowcytometer. Data were analyzed using Analysis of Varians (p<0,05 and using SPSS 16 for windows. The result showed that administration of  M. citrifolia crude extract in non infection groups was significantly increase the relative amounts T cell subsets (CD4+. Noni fruit extract can used as prevention therapy on infection disease of S. aureus bacteria because it contains active compounds that are anti-inflammation

  6. Morinda citrifolia Linn. Reduces Parasite Load and Modulates Cytokines and Extracellular Matrix Proteins in C57BL/6 Mice Infected with Leishmania (Leishmania) amazonensis.

    Science.gov (United States)

    Almeida-Souza, Fernando; Cardoso, Flávia de Oliveira; Souza, Bruno Vinicius da Conceição; do Valle, Tânia Zaverucha; de Sá, Joicy Cortez; Oliveira, Iara Dos Santos da Silva; de Souza, Celeste da Silva Freitas; Moragas Tellis, Carla Junqueira; Chagas, Maria do Socorro Dos Santos; Behrens, Maria Dutra; Abreu-Silva, Ana Lúcia; Calabrese, Kátia da Silva

    2016-08-01

    The absence of an effective vaccine and the debilitating chemotherapy for Leishmaniasis demonstrate the need for developing alternative treatments. Several studies conducted with Morinda citrifolia have shown various biological activities, including antileishmanial activity, however its mechanisms of action are unknown. This study aimed to analyze the in vivo activity of M. citrifolia fruit juice (Noni) against Leishmania (Leishmania) amazonensis in C57BL/6 mice. M. citrifolia fruit juice from the Brazilian Amazon has shown the same constitution of other juices produced around the world and liquid chromatography-mass spectrometry analysis identified five compounds: deacetylasperulosidic acid, asperulosidic acid, rutin, nonioside B and nonioside C. Daily intragastric treatment with Noni was carried out after 55 days of L. (L.) amazonensis infection in C57BL/6 mice. Parasitic loads, cytokine and extracellular protein matrix expressions of the lesion site were analyzed by qPCR. Histopathology of the lesion site, lymph nodes and liver were performed to evaluate the inflammatory processes. Cytokines and biochemical parameters of toxicity from sera were also evaluated. The Noni treatment at 500 mg.kg-1.day-1 for 60 days decreased the lesion size and parasitic load in the footpad infected with L. (L.) amazonensis. The site of infection also showed decreased inflammatory infiltrates and decreased cytokine expressions for IL-12, TNF-α, TGF-β and IL-10. On the other hand, Noni treatment enhanced the extracellular matrix protein expressions of collagen IV, fibronectin and laminin in the infected footpad as well collagen I and II, fibronectin and laminin in the mock-infected footpads. No toxicity was observed at the end of treatment. These data show the efficacy of Noni treatment.

  7. Morinda citrifolia Linn. Reduces Parasite Load and Modulates Cytokines and Extracellular Matrix Proteins in C57BL/6 Mice Infected with Leishmania (Leishmania) amazonensis

    Science.gov (United States)

    Almeida-Souza, Fernando; Cardoso, Flávia de Oliveira; Souza, Bruno Vinicius da Conceição; do Valle, Tânia Zaverucha; de Sá, Joicy Cortez; Oliveira, Iara dos Santos da Silva; de Souza, Celeste da Silva Freitas; Moragas Tellis, Carla Junqueira; Chagas, Maria do Socorro dos Santos; Behrens, Maria Dutra

    2016-01-01

    The absence of an effective vaccine and the debilitating chemotherapy for Leishmaniasis demonstrate the need for developing alternative treatments. Several studies conducted with Morinda citrifolia have shown various biological activities, including antileishmanial activity, however its mechanisms of action are unknown. This study aimed to analyze the in vivo activity of M. citrifolia fruit juice (Noni) against Leishmania (Leishmania) amazonensis in C57BL/6 mice. M. citrifolia fruit juice from the Brazilian Amazon has shown the same constitution of other juices produced around the world and liquid chromatography–mass spectrometry analysis identified five compounds: deacetylasperulosidic acid, asperulosidic acid, rutin, nonioside B and nonioside C. Daily intragastric treatment with Noni was carried out after 55 days of L. (L.) amazonensis infection in C57BL/6 mice. Parasitic loads, cytokine and extracellular protein matrix expressions of the lesion site were analyzed by qPCR. Histopathology of the lesion site, lymph nodes and liver were performed to evaluate the inflammatory processes. Cytokines and biochemical parameters of toxicity from sera were also evaluated. The Noni treatment at 500 mg.kg-1.day-1 for 60 days decreased the lesion size and parasitic load in the footpad infected with L. (L.) amazonensis. The site of infection also showed decreased inflammatory infiltrates and decreased cytokine expressions for IL-12, TNF-α, TGF-β and IL-10. On the other hand, Noni treatment enhanced the extracellular matrix protein expressions of collagen IV, fibronectin and laminin in the infected footpad as well collagen I and II, fibronectin and laminin in the mock-infected footpads. No toxicity was observed at the end of treatment. These data show the efficacy of Noni treatment. PMID:27579922

  8. Analysis of Organic Acids, Deacetyl Asperulosidic Acid and Polyphenolic Compounds as a Potential Tool for Characterization of Noni (Morinda citrifolia) Products.

    Science.gov (United States)

    Bittová, Miroslava; Hladůkova, Dita; Roblová, Vendula; Krácmar, Stanislav; Kubán, Petr; Kubán, Vlastimil

    2015-11-01

    Organic acids, deacetyl asperulosidic acid (DAA) and polyphenolic compounds in various noni (Morinda citrifolia L.) products (4 juices, 4 dry fruit powders and 2 capsules with dry fruit powder) were analyzed. Reversed-phase high-performance liquid chromatography (RP-HPLC) coupled with a variable wavelength detector (VWD) and electrospray ionization time-of-flight mass spectrometer (ESI-TOF MS) was applied for simultaneous analysis of organic acids (malic, lactic, citric and succinic acid) and DAA. An RP-HPLC method with diode-array detector (DAD) was developed for the analysis of polyphenolic compound content (rutin, catechin, quercitrin, kaempferol, gallic acid, caffeic acid and p-coumaric acid). The developed methods can contribute to better characterization of available noni products that is required from the consumers. In our study, we discovered significant dissimilarities in the content of DAA, citric acid and several phenolic compounds in some samples.

  9. ACTIVIDAD ANTIMICROBIANA EFECTOS MODULADORES EN MEDICAMENTOS ANTIFÚNGICOS Y ANTIBACTERIANOS DE LA FRUTO DE LA Morinda citrifolia L.

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    Saulo Relison TINTINO

    2015-07-01

    Full Text Available The surveillance of resistant microrganisms and the toxicity related with antimicrobial drugs enhances the necessity of research about new remedies. Morinda citrifolia is a plant commonly used as antibacterial, analgesic, and several other bioactivities, presenting aminoacids, anthraquinones, flavonoids, sterols, triterpenes and alkaloids. The aim of this work was evaluate the antimicrobial and the antibiotic-modulatory effect of the fruit extract. The extract was assayed alone and in association with antibiotic and antifungal drugs against clinical isolates by microdilution method. The fruit extract of M. citrifolia demonstrate an interesting modulatory activity, enhancing the effect of the assayed antibiotics against P. aeroginosa and E. coli, but not against Candida strains.

  10. EFFECT OF NONI (Morinda citrifolia EXTRACT ON TREATMENT OF ETHYLENE GLYCOL AND AMMONIUM CHLORIDE INDUCED KIDNEY DISEASE

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    R Bhavani

    2014-06-01

    Full Text Available Morinda citrifolia is one of the important medicinal plants having a lot of phytochemicals, which plays very important role in medicines. In this present investigation, the animals are induced to kidney stone by giving ethylene glycol mixed with water and given orally, and 0.5 % ammonium chloride are mixed with water and given orally for 28 days. The induced rats are treated with Noni (1 in 10 ml mixed with H2O and given to the rats for 28 days, and induced to kidney stone + ammonium chloride is treated with Noni extract mixed with water and given to the rats for 28 days. After the treatment the urinary parameters like creatinine, protein, calcium, oxalate, phosphate are decreased except magnesium, its level is increased and serum creatinine level is decreased. The results are shows the good medicinal properties of noni extract of Morinda citrifolia.

  11. Identification of major aroma compounds in the leaf of Morinda citrifolia Linn.

    Science.gov (United States)

    West, Brett J; Zhou, Bing-nan

    2008-10-01

    Morinda citrifolia, commonly named noni, has been used as food and as a folk medicine throughout the tropics. The use of the leaves to make hot water beverages is increasing in popularity, especially in Japan and the United States. To better understand the effects of processing on the content of the major aroma compounds, volatile oils were collected from samples of frozen, dried and roasted leaves by steam distillation and then analyzed by GC-MS. Drying of the leaves reduces the quantity of aroma compounds by more than half. Palmitic acid and E-phytol were identified as the major components of the volatile oil. With the exception of E-phytol, all of the known volatile compounds identified in the leaf samples were done so for the first time.

  12. The Green synthesis of gold nanoparticles using an aqueous root extract of Morinda citrifolia L.

    Science.gov (United States)

    Suman, T Y; Rajasree, S R Radhika; Ramkumar, R; Rajthilak, C; Perumal, P

    2014-01-24

    In the present work, we describe the synthesis of gold nanoparticles using an aqueous root extract of Morinda citrifolia. UV-vis spectroscopy, XRD, FTIR, FE-SEM, EDX and TEM were performed to characterize the formation of gold nanoparticles. The synthesized gold nanoparticles were characterized by a peak at 540 nm in the UV-vis spectrum. The XRD peaks at 38°, 44°, 64° and 77° can be indexed to the (111), (200), (220) and (311) Bragg's reflections of cubic structure of metallic gold, respectively. The FTIR result showed that extract containing protein might be responsible for the formation of the nanoparticles and may play an important role in the stabilization of the formed nanoparticles. FESEM images revealed that the particles were triangle and mostly spherical in shape. TEM images clearly revealed the size of the nanoparticles were 12.17-38.26 nm in size.

  13. Aktivitas Antimikroba Ekstrak Bubuk Buah Mengkudu (Morinda citrifolia L. Terhadap Pertumbuhan Bakteri Patogen

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    Nyoman Semadi Antara

    2015-04-01

    Full Text Available Penelitian yang dilakukan bertujuan untuk mengkaji aktivitas antimikroba ekstrak bubuk buah mengkudu (Morinda citrifolia L. terhadap pertumbuhan beberapa bakteri patogen. Bubuk buah mengkudu diolah dari buah mentah dan matang yang kemudian diekstrak menggunakan berbagai jenis larutan pengekstrak. Larutan pengekstrak yang dicoba dalam penelitian ini adalah etanol, petroleum eter (PE, dan aquades. Hasil percobaan menunjukkan bahwa ekstrak bubuk mengkudu mentah dan matang yang diekstraksi dengan pelarut etanol memperlihatkan spektrum penghambatan yang lebih luas dibandingkan dengan menggunakan larutan pengekstrak PE dan aquades. Ekstrak buah mengkudu yang menggunakan etanol sebagai larutan pengekstrak dapat menghambat pertumbuhan Escherichia coli, Salmonella typhi dan Bacillus cereus. Sementara ekstrak menggunakan pelarut PE hanya memberikan penghambatan terhadap B. cereus, dan ekstrak bubuk mengkudu yang diekstrak dengan aquades tidak terdeteksi memberikan penghambatan terhadap ketiga bakteri uji.Kata Kunci: antimikroba, mengkudu, larutan pengekstrak, bakteri pathogen.

  14. The Green synthesis of gold nanoparticles using an aqueous root extract of Morinda citrifolia L

    Science.gov (United States)

    Suman, T. Y.; Radhika Rajasree, S. R.; Ramkumar, R.; Rajthilak, C.; Perumal, P.

    2014-01-01

    In the present work, we describe the synthesis of gold nanoparticles using an aqueous root extract of Morinda citrifolia. UV-vis spectroscopy, XRD, FTIR, FE-SEM, EDX and TEM were performed to characterize the formation of gold nanoparticles. The synthesized gold nanoparticles were characterized by a peak at 540 nm in the UV-vis spectrum. The XRD peaks at 38°, 44°, 64° and 77° can be indexed to the (1 1 1), (2 0 0), (2 2 0) and (3 1 1) Bragg's reflections of cubic structure of metallic gold, respectively. The FTIR result showed that extract containing protein might be responsible for the formation of the nanoparticles and may play an important role in the stabilization of the formed nanoparticles. FESEM images revealed that the particles were triangle and mostly spherical in shape. TEM images clearly revealed the size of the nanoparticles were 12.17-38.26 nm in size.

  15. To trace the active compound in mengkudu (morinda citrifolia with anthelmintic acvtivity against Haemonchus contortus

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    T.B Murdiati

    2000-12-01

    Full Text Available Intestinal parasites such Haemonchus contortus is usually controlled by management improvement and regular administration of anthelmintic. However, there is an indication of H. contortus resistance to several anthelmintic available in the market, which makes medicinal plants as an alternative anthelmintic and mengkudu or noni fruit (Morinda citrifolia have been reported as an effective anthelmintic. To trace the active compounds responsible for anthelmintic activity against H. contortus, the mengkudu fruit was continuosly extracted into hexane, chloroform, metanol and water, followed by in-vitro study on the anthelmintic activity. The in-vitro anthelmintic activity was base on the ability of the extracts to kill the worm and the ability of the extracts to prevent egg development. The study suggested that chloroform fraction which contains alkaloid and anthraquinon have the highest anthelmintic activity and showed significant different compared to control (P≤ 0.05.

  16. Antimicrobial Activity of an Iridoid Rich Extract from Morinda citrifolia Fruit

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    Brett J. West

    2012-01-01

    Full Text Available The aim of the current study was to evaluate the antibacterial activity of iridoids in Morinda citrifolia (noni fruits. An iridoid rich extract from noni fruits was prepared and incubated with aliquots of twenty-four hour cultures of Candida albicans, Escherichia coli and Staphylococcus aureus at total iridoid concentrations of 0.096, 0.19, 0.45, 0.82 and 1.41 mg/mL. was monitored by Optical Density (OD at 600 nm. Iridoid concentration dependent decreases in cell growth were observed in all organisms. The results suggest that deacetylasperulosidic acid and asperulosidic acid, the major phytochemical constituents of noni fruit, possess antibacterial activity.

  17. Recovery of Phytochemical Components from Various Parts of Morinda citrifolia Extracts by Using Membrane Separator

    Science.gov (United States)

    Krishnaiah, Duduku; Sarbatly, Rosalam; Nah, Ng Lee

    In this study, extracts from various Morinda Citrifolia parts (leaf, fruit and root) by methanol was separated into permeate and retentate fractions using a membrane system equipped with a nanofiltration (NF) membrane. NF was carried on a ceramic membrane with MWCO of 5 kD. Effect of NF transmembrane pressure at 0.1, 0.12 and 0.17 bar was examined at constant temperature 45EC with constant flow rate. The influence of transmembrane pressure on the efficiency of antioxidant activity and total phenolic content of permeate retentate concentration was examined. The antioxidant activities of crude mengkudu extracts, NF permeate and retentate were evaluated by using the DPPH radical scavenging activity and total phenolic content.

  18. Iridoids from Morinda citrifolia%诺丽青果中的环烯醚萜

    Institute of Scientific and Technical Information of China (English)

    汤建国; 刘悍; 周忠玉; 刘吉开

    2009-01-01

    目的 研究诺丽(Morinda citrifolia)青果的化学成分.方法 利用硅胶、RP-18、Sephadex LH-20等柱色谱手段进行分离,根据理化性质和波谱分析鉴定化合物的结构.结果与结论 从其乙醇提取物的乙酸乙酯部分和正丁醇部分中分离得到5个环烯醚萜类化合物,分别鉴定为4-epi-dunnisinin(1)、车叶草苷(2)、borreriagenin(3)、去乙酰车叶草苷(4)和车叶草酸(5).其中,化合物1为新化合物.

  19. Morinda citrifolia leaf enhanced performance by improving angiogenesis, mitochondrial biogenesis, antioxidant, anti-inflammatory & stress responses.

    Science.gov (United States)

    Mohamad Shalan, Nor Aijratul Asikin; Mustapha, Noordin M; Mohamed, Suhaila

    2016-12-01

    Morinda citrifolia fruit, (noni), enhanced performances in athletes and post-menopausal women in clinical studies. This report shows the edible noni leaves water extract enhances performance in a weight-loaded swimming animal model better than the fruit or standardized green tea extract. The 4weeks study showed the extract (containing scopoletin and epicatechin) progressively prolonged the time to exhaustion by threefold longer than the control, fruit or tea extract. The extract improved (i) the mammalian antioxidant responses (MDA, GSH and SOD2 levels), (ii) tissue nutrient (glucose) and metabolite (lactate) management, (iii) stress hormone (cortisol) regulation; (iv) neurotransmitter (dopamine, noradrenaline, serotonin) expressions, transporter or receptor levels, (v) anti-inflammatory (IL4 & IL10) responses; (v) skeletal muscle angiogenesis (VEGFA) and (v) energy and mitochondrial biogenesis (via PGC, UCP3, NRF2, AMPK, MAPK1, and CAMK4). The ergogenic extract helped delay fatigue by enhancing energy production, regulation and efficiency, which suggests benefits for physical activities and disease recovery.

  20. Biosynthesis, characterization and cytotoxic effect of plant mediated silver nanoparticles using Morinda citrifolia root extract.

    Science.gov (United States)

    Suman, T Y; Radhika Rajasree, S R; Kanchana, A; Elizabeth, S Beena

    2013-06-01

    Silver has been used since time to control bodily infection, prevent food spoilage and heal wounds by preventing infection. The present study aims at an environmental friendly method of synthesizing silver nanoparticles, from the root of Morinda citrifolia; without involving chemical agents associated with environmental toxicity. The obtained nanoparticles were characterized by UV-vis absorption spectroscopy with an intense surface plasmon resonance band at 413 nm clearly reveals the formation of silver nanoparticles. Fourier transmission infra red spectroscopy (FTIR) showed nanopartilces were capped with plant compounds. Field emission-scanning electron microscopy (FE-SEM) and Transmission electron microscopy (TEM) showed that the spherical nature of the silver nanoparticles with a size of 30-55 nm. The X-ray diffraction spectrum XRD pattern clearly indicates that the silver nanoparticles formed in the present synthesis were crystalline in nature. In addition these biologically synthesized nanoparticles were also proved to exhibit excellent cytotoxic effect on HeLa cell.

  1. 诺丽(Morinda citrifolia Linn.)离体培养研究%STUDY ON CULTURE IN VITRO OF NONI (MORINDA CITRIFOLIA LINN.)

    Institute of Scientific and Technical Information of China (English)

    吴田; 蓝增全

    2011-01-01

    为诺丽遗传转化奠定基础及诺丽工厂化育苗提供依据,本实验以诺丽茎尖及带芽茎段为外植体,以3%MS培养基添加不同的激素组合进行离体培养,选择最佳的培养组合.实验结果表明,茎尖在MS或添加NAA(0.3 mg/L)的MS培养基中均可得到完整植株,且在后者中的长势更好.该研究成功建立了诺丽离体再生体系,为规模化生产及遗传转化提供了技术支撑.%To provided a theory foundation of genetic transformation and industrialized breeding of noni ( Morinda citrifolia Linn.), the perfect regeneration system was established using shoot tip and stem with buds of noni as explants in MS culture medium with different hormones combinations.The result showed that the complete plantlet was obtained by shoot tip cultured in MS or MS + NAA (0.3 mg/L), and the growth vigour was more better in the latter medium.The study established the regeneration system of noni, and provided a technology foundation for large scale production.

  2. Morinda citrifolia edible leaf extract enhanced immune response against lung cancer.

    Science.gov (United States)

    Lim, Swee-Ling; Goh, Yong-Meng; Noordin, M Mustapha; Rahman, Heshu S; Othman, Hemn H; Abu Bakar, Nurul Ain; Mohamed, Suhaila

    2016-02-01

    Lung cancer causes 1.4 million deaths annually. In the search for functional foods as complementary therapies against lung cancer, the immuno-stimulatory properties of the vegetable Morinda citrifolia leaves were investigated and compared with the anti-cancer drug erlotinib. Lung tumour-induced BALB/c mice were fed with 150 mg kg(-1) or 300 mg kg(-1) body weight of the leaf extract, or erlotinib (50 mg kg(-1) body-weight) for 21 days. The 300 mg kg(-1) body weight extract significantly (and dose-dependently) suppressed lung tumour growth; the extract worked more effectively than the 50 mg kg(-1) body weight erlotinib treatment. The extract significantly increased blood lymphocyte counts, and spleen tissue B cells, T cells and natural killer cells, and reduced the epidermal growth factor receptor (EGFR) which is a lung adenocarcinoma biomarker. The extract also suppressed the cyclooxygenase 2 (COX2) inflammatory markers, and enhanced the tumour suppressor gene (phosphatase and tensin homolog, PTEN). It inhibited tumour growth cellular gene (transformed mouse 3T3 cell double minute 2 (MDM2), V-raf-leukemia viral oncogene 1 (RAF1), and mechanistic target of rapamycin (MTOR)) mRNA expression in the tumours. The extract is rich in scopoletin and epicatechin, which are the main phenolic compounds. The 300 mg kg(-1)Morinda citrifolia leaf 50% ethanolic extract showed promising potential as a complementary therapeutic dietary supplement which was more effective than the 50 mg kg(-1) erlotinib in suppressing lung adenocarcinoma. Part of the mechanisms involved enhancing immune responses, suppressing proliferation and interfering with various tumour growth signalling pathways.

  3. Pengaruh Ekstrak Buah Morinda Citrifolia Linn Terhadap Kualitas, Kuantitas Sperma Dan Kadar Malondialdehyde Testis Tikus Wistar Diabetes Mellitus

    OpenAIRE

    Meldawati

    2011-01-01

    Hyperglycemia in Diabetes Mellitus causes increased levels of free radicals. Autooxydation in the process of hyperglycemia triggers the formation of free radicals. Free radicals can damage cell membranes, a lipid peroxide or Malondialdehyde (MDA), if was continued lead to system damage cell membranes and cell death. Morinda Citrifolia Linn contain secondary metabolic compounds that contain nutrients that are also diverse as Vitamins A, C, Niacin, Thiamine and Riboflavin, and minerals such of ...

  4. Anti-proliferative and antioxidative activities of Thai noni/Yor (Morinda citrifolia Linn.) leaf extract.

    Science.gov (United States)

    Thani, Wasina; Vallisuta, Omboon; Siripong, Pongpan; Ruangwises, Nongluck

    2010-03-01

    In this study the leaves of the Thai noni/Yor, (Morinda citrifolia Linn.) were extracted by several methods and evaluated against human cancer cell lines: KB (human epidermoid carcinoma), HeLa (human cervical carcinoma), MCF-7 (human breast carcinoma) and HepG2 (human hepatocellular carcinoma) cell lines as well as a Vero (African green monkey kidney) cell line, employing the MTT colorimetric method, comparing it to damnacanthal, rutin, and scopoletin. The dichloromethane extract of the fresh leaf showed a better inhibitory effect against KB and HeLa cells with IC50 values of 21.67 and 68.50 microg/ml, respectively. The dichloromethane extract of dried leaves revealed cytotoxicity against the KB cell line with an IC50 value of 39.00 microg/ml. Other extracts, as well as rutin and scopoletin, showed reduced anti-proliferative effects on all cancer cell lines (IC50 103 to over 600 microg/ml). Interestingly, the damnacanthal had potent cytotoxicity against all cancer cell lines and Vero cell lines. These results suggest Thai noni extracts may be safer than the pure compounds, due to their higher safety ratios, which is a good indicator for possible cancer treatment. Several non-aqueous extracts from the leaves showed antioxidant properties, giving IC50 values of 0.20-0.35 mg/ml. It can be concluded the leaves of M. citrifolia may have benefit as a food supplement for chemoprevention against epidermoid and cervical cancers.

  5. Optimisation of spray drying operating conditions of Morinda citrifolia L. fruit extract using response surface methodology

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    Duduku Krishnaiah

    2015-01-01

    Full Text Available A conventional solvent extract of Morinda citrifolia L. fruit was spray dried using adjuvant maltodextrin (5 wt.%. Spray drying was carried out according to the D-optimal design, and the independent variables selected were temperature and Mcore/Mwall. The spray drying process was optimised by using response surface methodology (RSM for four different responses: moisture content (MC, DPPH scavenging activity, total phenolic content (TPC, and total flavonoid (TF. The effects of temperature and of the core to wall material ratio were found to be significant for all responses. The optimal spray drying condition for maltodextrin as binding material was found to be 1:1.5 (Mcore/Mwall, volume ratio of M. citrifolia L. extract to additive solution at 95 °C. The experimental values of the response variables correspond well to the predicted values. The microparticles obtained in this study represent an interesting food additive for incorporation into functional foods due to the presence of antioxidants.

  6. Antioxidant and antiangiogenic activities of the essential oils of Myristica fragrans and Morinda citrifolia

    Institute of Scientific and Technical Information of China (English)

    Suthagar Pillai Piaru; Roziahanim Mahmud; Amin Malik Shah Abdul Majid; Zeyad Daoud Mahmoud Nassar

    2012-01-01

    ABSTRACT Objective:Toinvestigate the anti-angiogenic activity and antioxidant properties ofMyristica fragrans (M. fragrans) (nutmeg) andMorinda citrifolia (M. citrifolia)(mengkudu) oils. Methods: The nutmeg and megkudu essential oils were obtained by steam distillation. The antioxidant activities of both essential oils were determined by beta-carotene/linoleic acid bleaching assay and reducing power while the anti-angiogenic activity was investigated using rat aortic ring assay using various concentrations.Results:The results showed that nutmeg oil has higher antioxidant activity than mengkudu oil. The nutmeg oil effectively inhibited the oxidation of linoleic acid with (88.68±0.1)% while the inhibition percentage of oxidation of linoleic acid of the mengkudu oil is (69.44±0.4)%. The nutmeg oil and mengkudu oil showed reducing power with anEC50 value of 181.4 μg/mL and 3 043.0μg/mL, respectively. The antiangiogenic activity of nutmeg oil showed significant antiangiogenic activity withIC50 of77.64μg/mL comparing to mengkudu oil which exhibits IC50 of109.30 μg/mL.Conclusion: Bioactive compound(s) will be isolated from the nutmeg essential oil to be developed as antiangiogenic drugs.

  7. Studies on the Micropropagation of Noni (Morinda citrifolia L.)%海巴戟(Morinda citrifolia L.)组织培养研究

    Institute of Scientific and Technical Information of China (English)

    黄骐; 何文锦; 叶冰莹; 陈由强; 陈如凯

    2006-01-01

    以海巴戟(Morinda citrifolia L.)种子为试材,在不剥除种皮的情况下,在MS无激素培养基上播种1年内未见发芽,在剥除种皮的情况下,在MS无激素培养基上发芽率最高,50 d内可达75%.海巴戟子叶和下胚轴均能单独由细胞分裂素BA 0.7~2.0 mg/L诱导不定芽发生,不定芽可直接从外植体发生,也可从愈伤组织发生,添加生长素NAA 0.05~0.1 mg/L则完全抑制不定芽发生,同时强烈促进愈伤组织生长和不定根发生.带芽茎段在BA1.5 mg/L配合低浓度生长素时均能通过腋芽萌发和不定芽发生而增殖.芽梢在NAA 0.1 mg/L、IBA 0.1mg/L或IAA0.1 mg/L均有根群发生,但NAA0.1 mg/L诱导生根时切口愈伤组织较多,部分不定根由愈伤组织发生,而IAA 0.1 mg/L诱导生根时根群欠发达,以IBA 0.1 mg/L最佳.

  8. Studies on the Micropropagation of Noni(Morinda citrifolia L.)%诺丽(Morinda citrifolia L.)离体快速繁殖研究

    Institute of Scientific and Technical Information of China (English)

    黄骐; 何文锦; 叶冰莹; 陈由强; 陈如凯

    2007-01-01

    以诺丽(Morinda citrifolia L.)种子为试材.诺丽子叶和下胚轴均能单独由细胞分裂素BA 0.7~2.0 mg/L诱导不定芽发生,不定芽可直接从外植体发生,也可从愈伤组织发生, 添加生长素NAA 0.05~0.1 mg/L则完全抑制不定芽发生,同时强烈促进愈伤组织和不定根发生.茎段在BA 0.5~5.0 mg/L或ZT 1.5 mg/L或KN 1.5 mg/L时均能通过腋芽萌发和不定芽发生而增殖.芽梢在NAA 0.1 mg/L、IBA 0.1 mg/L或IAA 0.1 mg/L均有根群发生,但NAA 0.1 mg/L诱导生根时切口愈伤组织较多,部分不定根由愈伤组织发生,而IAA 0.1 mg/L诱导生根时根群欠发达,以IBA 0.1 mg/L为最佳.试管苗移植到泥炭上10 d后即可在全光照下生长.

  9. Safety evaluation of Morinda citrifolia (noni leaves extract: assessment of genotoxicity, oral short term and subchronic toxicity

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    Alicia Lagarto

    2013-02-01

    Full Text Available Morinda citrifolia L (noni is an evergreen or small tree that grows in many tropical regions of the world. The use of the noni leaves has not been so studied however; there are reports of its pharmacological benefits. Aims: The objective of this investigation was to assess the genotoxicity, short-term, and subchronic oral toxicity of Morinda citrifolia L leaves aqueous extract. Methods: The genotoxicity of the M. citrifolia extract was investigated by measuring the frequency of micronuclei in mice bone marrow cells. The animals were treated with three doses of the extract (500, 1000, and 2000 mg/kg. For short-term toxicity, both sexes Wistar rats received 1000 mg/kg/day for 28 days. Animals were sacrificed for hematological and biochemical evaluation. For the subchronic study, Wistar rats were administered with three doses of M. citrifolia extract (100, 300, and 1000 mg/kg by oral route for 90 days. Mortalities, clinical signs, body weight changes, food and water consumption, hematological and biochemical parameters, gross findings, organ weights, and histological examination were monitored during the study period. Results: Genotoxicity and short-term toxicity test resulted in absence of toxicity at doses between 500 and 2000 mg/kg. Significant differences were observed in hemoglobin, and differential leukocyte count after subchronic dosing of the extract. Histology evaluation did not reveal treatment-related abnormalities. Variations observed were within to normal range and reversible. Conclusions: In summary, 1000 mg/kg orally was the NOAEL for M. citrifolia extract for effects other than transient variations in some hematological parameters within normal range. [J Intercult Ethnopharmacol 2013; 2(1.000: 15-22

  10. Mengkudu (Morinda citrifolia Linn. gel affect on post-extraction fibroblast acceleration

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    Christian Khoswanto

    2010-03-01

    Full Text Available Background: Tooth extraction is one of treatment frequently done by dentists in clinics, hospital, and even private practices. One thing that is needed to be observed after the treatment is the speed of wound recovery process. Mengkudu is commonly used as medicinal treatments, some of them to heal wounds, but there had never been research of the use of mengkudu fruit on wound recovery after tooth extraction. Purpose: The aim of this study was to investigate the effect of mengkudu gel in accelerating the escalation of fibroblast post tooth extraction on Dawley rats. Method: This study was used post test only control group design. Thirty male Dawley rats weigh between 250-300 grams, 3 months of age are being used. Tooth extraction is being done on lower left incisor. The 30 rats are divided into three groups, there are mengkudu (Morinda citrifolia Linn. gel, poviclone iodine, and control group. The data were analyzed statistically using One-Way ANOVA and LSD. Result: The result of every tested group with Kolmogorof-Smirnov test showed p > 0.05. Examination showed there was significant difference in fibroblast amount between the group with mengkudu gel and two other groups (p < 0.05. Conclusion: The application of mengkudu gel can accelerate the escalation of fibroblast after the tooth extraction on Dawley rats.Latar Belakang: Ekstraksi gigi merupakan perawatan yang sering dilakukan oleh dokter gigi baik di klinik, rumah sakit, dan praktekpribadi. Satu hal yang perlu diperhatikan setelahpencabutan gigi adalah kecepatanpenyembuhan luka bekas cabut. Mengkudu merupakan bahan yang sering digunakan untukpengobatan, salah satu diantaranya adalah untukpenyembuhan luka, namunpenelitian untuk kesembuhan luka pasca cabut gigi yang menggunakan mengkudu belum pernah dilakukan sebelumnya. Tujuan: Tujuanpenelitian ini untuk mengetahui efek gel mengkudu dalam mempercepat peningkatan jumlah fibroblas setelah pencabutan gigi tikus Dawley. Metode: Penelitian ini

  11. Morinda citrifolia Linn: potencialidades para su utilización en la salud humana

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    Nirda E González Lavaut

    2003-12-01

    Full Text Available Noni es el nombre común de la Morinda citrifolia Linn. originaria de la Polinesia, Malasia, Australia, India y el Sudeste de Asia, pero se ha extendido a casi todas las regiones del mundo. En este trabajo se muestran los aspectos más importantes que sobre esta planta aparecen en publicaciones, fundamentalmente de la vía Internet y del Chemical Abstracts en los últimos 5 años, que permitan valorar las potencialidades para su uso en la salud humana. En la medicina tradicional las frutas, flores, hojas, corteza y raíz de esta planta han sido utilizadas para diversos propósitos medicinales. Entre las afecciones más tratadas se encuentran la alergia, artritis, asma, cáncer, diabetes, depresión, debilidad física, desórdenes menstruales, obesidad y estrés. Si bien se ha encontrado en los extractos de varias partes de la planta una elevada cantidad de componentes e incluso algunos se han llegado a aislar, se considera que la acción farmacológica y beneficiosa se alcanza únicamente por el efecto sinérgico de todos los componentes.Noni is the common name given to Morinda citrifolia Linn. It is originating from Polinesia, Malasia, Australia, India and Southeast Asia, but it has extended to almost every region of the world. In this paper, the most important aspects of this plant appearing in publications, Internet and the Chemical Abstracts in the last 5 years that allow to assess the potentialities for its use in human health, are shown. In traditional medicine, the fruit, flowers, leaves, cortex and root of this plant have been used for diverse medicinal purposes. Allergy, arthritis, asthma, cancer, diabetes, depression, physical weakness, menstrual disorders, obesity and stress are among the most treated affections. Although in the extracts of various parts of the plant, a high number of components has been found and even some of them have been isolated, it is considered that the pharmacological and beneficial action is only reached by

  12. Anxiolytic, sedative, and hypnotic activities of aqueous extract of Morinda citrifolia fruit.

    Science.gov (United States)

    Kannan, Sridharan; Manickam, Shanti; RajaMohammed, Meher Ali

    2014-04-01

    Morinda citrifolia (Indian mulberry or noni) fruit has been long used as a folk medicine for a wide range of health purposes as it is claimed to have analgesic, antiinflammatory, antioxidant, detoxifier, and cell-rejuvenator properties. A recent study has revealed central nervous system suppressant nature of its extract. Hence, the present study has evaluated the anxiolytic, sedative, and hypnotic effects of the aqueous extracts of Morinda citrifolia in rodents in comparison to diazepam. Anxiety was assessed by 'Isolation-induced aggression' model, sedation by 'Spontaneous locomotor activity using actophotometer' and hypnotic activity by 'Prolongation of ketamine-induced sleeping time'. Six male mice were used for each of the groups and postdose, all the six that received diazepam had shown an inhibition of aggression, whereas in the test group, five of six mice and none in the control group had shown an inhibition of aggression (P = 0.0007). Similarly, for the sedative activity, the total number of spontaneous locomotor activity at 30 min following drug administration was found to be 364.67 ± 10.74, 123.16 ± 8.33, and 196.67 ± 3.7, while at 60 min it was found to be 209 ± 12.98, 49 ± 5.78, and 92 ± 2.5 (mean ± SD) for the control, standard, and test groups of mice respectively (P < 0.001). Hypnotic activity was measured by prolongation of ketamine-induced sleeping time wherein the onset and duration of loss of righting reflex were compared among each group of mice. The time in minutes for the onset in control, standard, and test groups was 4.01 ± 0.22, 1.23 ± 0.05, and 2.23 ± 0.07, respectively. The duration of loss of righting reflex was 44.23 ± 0.59, 56.03 ± 1.34, and 50.57 ± 0.36, respectively. Both these were statistically significant (P < 0.001). However, more clinical studies are needed to assess the long-term effects of the extract in humans.

  13. Anxiolytic, sedative, and hypnotic activities of aqueous extract of Morinda citrifolia fruit

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    Sridharan Kannan

    2014-01-01

    Full Text Available Morinda citrifolia (Indian mulberry or noni fruit has been long used as a folk medicine for a wide range of health purposes as it is claimed to have analgesic, antiinflammatory, antioxidant, detoxifier, and cell-rejuvenator properties. A recent study has revealed central nervous system suppressant nature of its extract. Hence, the present study has evaluated the anxiolytic, sedative, and hypnotic effects of the aqueous extracts of Morinda citrifolia in rodents in comparison to diazepam. Anxiety was assessed by ′Isolation-induced aggression′ model, sedation by ′Spontaneous locomotor activity using actophotometer′ and hypnotic activity by ′Prolongation of ketamine-induced sleeping time′. Six male mice were used for each of the groups and postdose, all the six that received diazepam had shown an inhibition of aggression, whereas in the test group, five of six mice and none in the control group had shown an inhibition of aggression (P = 0.0007. Similarly, for the sedative activity, the total number of spontaneous locomotor activity at 30 min following drug administration was found to be 364.67 + 10.74, 123.16 + 8.33, and 196.67 + 3.7, while at 60 min it was found to be 209 + 12.98, 49 + 5.78, and 92 + 2.5 (mean + SD for the control, standard, and test groups of mice respectively (P < 0.001. Hypnotic activity was measured by prolongation of ketamine-induced sleeping time wherein the onset and duration of loss of righting reflex were compared among each group of mice. The time in minutes for the onset in control, standard, and test groups was 4.01 + 0.22, 1.23 + 0.05, and 2.23 + 0.07, respectively. The duration of loss of righting reflex was 44.23 + 0.59, 56.03 + 1.34, and 50.57 + 0.36, respectively. Both these were statistically significant (P < 0.001. However, more clinical studies are needed to assess the long-term effects of the extract in humans.

  14. Anti-allergic activity of the Morinda citrifolia extract and its constituents

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    Kazuya Murata

    2014-01-01

    Full Text Available Background: Morinda citrifolia (Rubiaceae, commonly known as noni is distributed throughout tropical and sub-tropical regions of the world. Anti-allergic effects of noni have not been reported despite the clinical usage as an anti-allergic agent. Materials and Methods: To investigate the anti-allergic effects of the 50% ethanolic extract of M. citrifolia fruits and leaves (MCF-ext and MCL-ext, dinitrofluorobenzene (DNFB-induced triphasic cutaneous reaction and picryl chloride-induced contact dermatitis (PC-CD tests were performed. Results: In DNFB-induced triphasic cutaneous reaction, oral administration of MCF-ext and MCL-ext exhibited dose-dependent inhibition of cutaneous reaction at 1 h (immediate phase response after the DNFB challenge. MCF-ext also inhibited ear swelling at 24 h (late phase response and 8 days (very late phase response after the DNFB challenge. The effect of MCL-ext on the immediate phase response was attributed to the anti-degranulation from RBL-2H3 cells, while MCF-ext had no significant effect on degranulation. The active components of anti-degranulation activity in MCL-ext were determined to be ursolic acid, rutin and kaempferol-3-O-α-L-rhamnopyranosyl-(1→6-β-D-glucopyranoside. In the PC-CD test, both MCF-ext and MCL-ext showed an anti-swelling effect but the potency of MCF-ext was stronger than MCL-ext. Conclusion: These data suggest that noni fruits and leaves can be a daily consumable material for the prevention of allergic symptoms.

  15. Morinda citrifolia Linn. (Noni and Its Potential in Obesity-Related Metabolic Dysfunction

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    Aline Carla Inada

    2017-05-01

    Full Text Available Cultural and economic shifts in the early 19th century led to the rapid development of companies that made good profits from technologically-produced commodities. In this way, some habits changed in society, such as the overconsumption of processed and micronutrient-poor foods and devices that gave rise to a sedentary lifestyle. These factors influenced host-microbiome interactions which, in turn, mediated the etiopathogenesis of “new-era” disorders and diseases, which are closely related, such as obesity, type 2 diabetes mellitus, non-alcoholic fatty liver disease, hypertension, and inflammatory bowel disease, which are characterized by chronic dysregulation of metabolic and immune processes. These pathological conditions require novel and effective therapeutic approaches. Morinda citrifolia (noni is well known as a traditional healing plant due to its medicinal properties. Thus, many studies have been conducted to understand its bioactive compounds and their mechanisms of action. However, in obesity and obesity-related metabolic (dysfunction syndrome, other studies are necessary to better elucidate noni’s mechanisms of action, mainly due to the complexity of the pathophysiology of obesity and its metabolic dysfunction. In this review, we summarize not only the clinical effects, but also important cell signaling pathways in in vivo and in vitro assays of potent bioactive compounds present in the noni plant which have been reported in studies of obesity and obesity-associated metabolic dysfunction.

  16. Evaluation of the Wound-Healing Activity of Ethanolic Extract of Morinda citrifolia L. Leaf

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    B. Shivananda Nayak

    2009-01-01

    Full Text Available Morinda citrifolia L. (noni is one of the most important traditional Polynesian medicinal plants. The primary indigenous use of this plant appears to be of the leaves, as a topical treatment for wound healing. The ethanol extract of noni leaves (150 mg kg−1 day−1 was used to evaluate the wound-healing activity on rats, using excision and dead space wound models. Animals were randomly divided into two groups of six for each model. Test group animals in each model were treated with the ethanol extract of noni orally by mixing in drinking water and the control group animals were maintained with plain drinking water. Healing was assessed by the rate of wound contraction, time until complete epithelialization, granulation tissue weight and hydoxyproline content. On day 11, the extract-treated animals exhibited 71% reduction in the wound area when compared with controls which exhibited 57%. The granulation tissue weight and hydroxyproline content in the dead space wounds were also increased significantly in noni-treated animals compared with controls (P < 0.002. Enhanced wound contraction, decreased epithelialization time, increased hydroxyproline content and histological characteristics suggest that noni leaf extract may have therapeutic benefits in wound healing.

  17. Induction of mitochondrial-mediated apoptosis by Morinda citrifolia (Noni) in human cervical cancer cells.

    Science.gov (United States)

    Gupta, Rakesh Kumar; Banerjee, Ayan; Pathak, Suajta; Sharma, Chandresh; Singh, Neeta

    2013-01-01

    Cervical cancer is the second most common cause of cancer in women and has a high mortality rate. Cisplatin, an antitumor agent, is generally used for its treatment. However, the administration of cisplatin is associated with side effects and intrinsic resistance. Morinda citrifolia (Noni), a natural plant product, has been shown to have anti-cancer properties. In this study, we used Noni, cisplatin, and the two in combination to study their cytotoxic and apoptosis-inducing effects in cervical cancer HeLa and SiHa cell lines. We demonstrate here, that Noni/Cisplatin by themselves and their combination were able to induce apoptosis in both these cell lines. Cisplatin showed slightly higher cell killing as compared to Noni and their combination showed additive effects. The observed apoptosis appeared to be mediated particularly through the up-regulation of p53 and pro-apoptotic Bax proteins, as well as down- regulation of the anti-apoptotic Bcl-2, Bcl-XL proteins and survivin. Augmentation in the activity of caspase-9 and -3 was also observed, suggesting the involvement of the intrinsic mitochondrial pathway of apoptosis for both Noni and Cisplatin in HeLa and SiHa cell lines.

  18. First isolation and antinociceptive activity of a lipid transfer protein from noni (Morinda citrifolia) seeds.

    Science.gov (United States)

    Campos, Dyély C O; Costa, Andrea S; Lima, Amanda D R; Silva, Fredy D A; Lobo, Marina D P; Monteiro-Moreira, Ana Cristina O; Moreira, Renato A; Leal, Luzia K A M; Miron, Diogo; Vasconcelos, Ilka M; Oliveira, Hermógenes D

    2016-05-01

    In this study a novel heat-stable lipid transfer protein, designated McLTP1, was purified from noni (Morinda citrifolia L.) seeds, using four purification steps which resulted in a high-purified protein yield (72 mg McLTP1 from 100g of noni seeds). McLTP1 exhibited molecular masses of 9.450 and 9.466 kDa, determined by electrospray ionisation mass spectrometry. The N-terminal sequence of McLTP1 (AVPCGQVSSALSPCMSYLTGGGDDPEARCCAGV), as analysed by NCBI-BLAST database, revealed a high degree of identity with other reported plant lipid transfer proteins. In addition, this protein proved to be resistant to pepsin, trypsin and chymotrypsin digestion. McLTP1 given intraperitoneally (1, 2, 4 and 8 mg/kg) and orally (8 mg/kg) caused an inhibition of the writhing response induced by acetic acid in mice. This protein displayed thermostability, retaining 100% of its antinociceptive activity after 30 min incubation at 80 °C. Pretreatment of mice with McLTP1 (8 mg/kg, i.p. and p.o.) also decreased neurogenic and inflammatory phases of nociception in the formalin test. Naloxone (2 mg/kg, i.p.) antagonised the antinociceptive effect of McLTP1 suggesting that the opioid mechanisms mediate the analgesic properties of this protein.

  19. Flow injection spectrophotometry using natural reagent from Morinda citrifolia root for determination of aluminium in tea.

    Science.gov (United States)

    Tontrong, Sopa; Khonyoung, Supada; Jakmunee, Jaroon

    2012-05-01

    A flow injection (FI) spectrophotometric method with using natural reagent extracted from Morinda citrifolia root has been developed for determination of aluminium. The extract contained anthraquinone compounds which could react with Al(3+) to form reddish complexes which had maximum absorption wavelength at 499.0nm. The extract could be used as a reagent in FI system without further purification to obtain pure compound. A sensitive method for determination of aluminium in concentration range of 0.1-1.0mgL(-1), with detection limit of 0.05mgL(-1) was achieved. Relative standard deviations of 1.2% and 1.7% were obtained for the determination of 0.1 and 0.6mgL(-1) Al(3+) (n=11). Sample throughput of 35h(-1) was achieved with the consumption of 3mL each of carrier and reagent solutions per injection. The developed method was successfully applied to tea samples, validated by the FAAS standard method. The method is simple, fast, economical and could be classified as a greener analytical method.

  20. Aqueous Morinda citrifolia Leaves Extract Enhancing Glutathione Peroxidase Activity and α2-Macroglobulin Gene Expression on Macrobrachium rosenbergii

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    Atika Marisa Halim

    2017-06-01

    Full Text Available Morinda citrifolia, known commercially as noni is often used for enhancing immunity, these plant-rich phenolic compound with antioxidant properties. In the present study, Macrobrachium rosenbergii were fed diets containing aqueous M. citrifolia leaves extract (AMLE at 0.6, 4 and 6 g kg-1. Glutathione peroxidase (GPx and α2-macroglubulin (α2-M  activity were conducted to measure an immune parameter, which was evaluated before and after 7, 21, 35, 49 and 63 days of feeding trial. The results showed that after 63 days of feeding treatment, significantly increased in GPx activity. Moreover, the gene expressions of α2-macroglubulin was significantly upregulated. These results recommend that administration of AMLE can be used as an immunostimulant and regulated immune response and immune gene expression in M. rosenbergii.

  1. PEMURNIAN PROTEASE DARI BUAH DAN DAUN MENGKUDU (Morinda citrifolia L. [Purification of Proteases from Fruits and Leaves of Noni (Morinda citrifolia L.

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    Dwi Ishartani1,2*

    2011-06-01

    Full Text Available Proteases have been widely used in cancer treatment, wounds healing, overcoming digestion disorder and other modern pharmaceutical applications. Proteases may be present in fruits and leaves of noni (Morinda citrifolia L. since the plant has been used traditionally in wound healing. This research aimed to purify proteases from noni’s fruits and leaves at two maturity stages, i.e. fruits with green-yellow (TK2 and white-yellow (TK4 skin colour, also leaves from shoot and base. Proteases were purified through several steps consisting of extraction, precipitation using saturated ammonium sulfate and dialysis followed by electrophoresis under denaturing conditions (SDS-PAGE and zymography. The specific activity of the four extracts showed different trend during purification. The specific activity of TK2 fruit, shoot and base leaves decreased whereas TK4 fruits increased. TK2 crude extract had a higher specific activity (3.79 U/mg than the other crude extracts. SDS-PAGE and zymogram using 12% acrylamide indicated that the dialysates were not pure proteases. The molecular weight profiles of the TK2 dialysates were similar to TK4 dialysates, while those of shoot leaves dialysates were similar to the base leaves dialysates. The SDS-PAGE separated the enzymes in the fruit dialysates into several bands of polypeptides,i.e. 24-26kDa, 14-15kDa, 12-13kDa and the smaller ones, and also separated the enzymes in the dialysates of leaves into two bands, 70kDa and 58-61.5kDa. Protease bands detected in the zymogram of fruits were estimated at ~25kDa, ~27kDa, and 37-38kDa, whereas of those of leaves were estimated at ~29kDa and ~50kDa. The ~25kDa and ~29kDa protein bands appearing in zymogram were similar to papain’s and bromelain’s molecular weight.

  2. A double-blind, randomized, clinical trial on the antileishmanial activity of a Morinda citrifolia (Noni) stem extract and its major constituents.

    Science.gov (United States)

    Sattar, Fouzia A; Ahmed, Fayaz; Ahmed, Nadeem; Sattar, Samina A; Malghani, Muhammad A K; Choudhary, Muhammad I

    2012-02-01

    A controlled study was conducted to determine the efficiency of a topical ointment prepared from the stem extract of Morinda citrifolia against cutaneous leishmaniasis. Similarly, the in vitro antileishmanial activity of morindicone and morinthone isolated from the extract were investigated against Leishmania major. These compounds displayed good activity. Out of 40 patients, 50% showed an excellent response and 30% exhibited good improvement.

  3. Anthelmintic action of Morinda citrifolia (noni on Heterakis gallinarumAção anti-helmíntica da Morinda citrifolia (noni sobre Heterakis gallinarum

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    Danilo Rodrigues Barros Brito

    2013-09-01

    Full Text Available The anthelmintic effect of Morinda citrifolia (noni on Heterakis gallinarum was evaluated in chicken naturally infected. The anthelmintic activity in vitro was determined in adults helminthes in disposable petri dishes, containing Tyrode solution, pre warmed in which aqueous or ethanolic extracts were added. The material was maintained in a BOD at 37ºC (±1. The aqueous and ethanolic extracts presented the following concentrations: 1.69; 3.37; 6.74; 13.48 e 26.96 mg.mL-1 and 4.17; 8.34; 16.68; 33.36 e 66.72 mg.mL-1, respectively. It was used as positive control, a solution of tetrahidrate citrate of piperazin in the concentration of 50 mg/mL. The anthelmintic activity in vivo was determined by the administration of aqueous or ethanolic extracts (10 mL/Kg/PV during three consecutive days. The feces were collected during four days in each group, washed in water and sifted. In the fifth day post-treatment, the chickens were slaughtered and necropsy was performed in order to count and identify remaining helminthes. The data were analyzed by the Student-Newman-Keuls test. In the in vivo test there was no significant difference between the aqueous extract and the control group (water (p>0.05 in the elimination of H. gallinarum. The ethanolic extract presented an elimination of 20.35%, differing statistically from the control group (p A ação anti-helmíntica da Morinda citrifolia (noni sobre Heterakis gallinarum foi avaliada em galinhas poedeiras naturalmente infectadas. A atividade anti-helmíntica in vitro foi determinada em helmintos adultos colocados em placas de petri descartáveis, contendo solução Tyrode pré-aquecida, nas quais foi adicionado o extrato aquoso ou etanólico e mantidas em BOD a uma temperatura de 37oC (±1. Os extratos aquoso e etanólico foram usados nas seguintes concentrações: 1,69; 3,37; 6,74; 13,48 e 26,96 mg.mL-1 e 4,17; 8,34; 16,68; 33,36 e 66,72 mg.mL-1, respectivamente. Como controle positivo usou-se uma solu

  4. Two New Saccharide Fatty Acid Esters from the Fruit of Morinda citrifolia L. and Their ABTS Radical Scavenging Activities

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    Hong-Cai Zhang

    2014-01-01

    Full Text Available Two n ew saccharide fatty acid esters (1 and (2, and six other compounds were isolated from the fruit of Morinda citrifolia L. (Rubiaceae The structures were established as (2E-oct-2-enoate-2-O-β-D - glucopyranosyl-β-D-glucopyranoside(1, (2E-2,6-dimethyl -6-hydroxyl-oct-2,7-dienoate-2-O-β-D-glucopyranosyl-β-D-glucopyranoside(2, saccharumoside C(3, O-β-D-apiofuranosyl-(1→6-O-β-D-glucopyranosides of 3-methyl-2-butenol(4, 3-methyl-but-2-en-1-yl β-D-glucopyranosyl (1→6-β-D-glucopyranoside(5, benzyl - glucopyranoside(6, Hexyl-O-β-D-glucopyranoside(7, and caproic acid(8. Compound 1 and 2 showed moderate activity against ABTS (2,2’-azino-bis(3-ethylbenzothiazoline-6-sulphonate radical in concentration of 0.1-3.2 mg/mL. Morinda citrifolia L., saccharide fatty acid ester, ABTS radical scavenging activity

  5. The morphological characterization of the dry seeds and reserve mobilization during germination in Morinda citrifolia L.

    Directory of Open Access Journals (Sweden)

    Stelamaris de Oliveira Paula

    2016-09-01

    Full Text Available ABSTRACT Information about the morphology, chemical composition and reserve mobilization is important in understanding the establishment of native and exotic species. The purpose of this study was to describe the morphology, chemical composition, and mobilization of reserves during germination of noni (Morinda citrifolia L.. Biometric and morphological analyzes were performed with 100 randomly selected dried seeds. Other seeds were treated with sulfuric acid PA and soaked in Petri dishes. Collected seeds of five different times of germination were used for cytochemical and chemical analysis. For cytochemical analysis, the cuts of 5μm were submitted to dyes TB at pH 4.0; XP at pH 2.5, reaction of PAS and Sudan IV. The seeds were crushed for chemical analysis with lipids, proteins and soluble sugars extraction that were determined gravimetrically, by the Bradford method and the Antrona method, respectively. The fatty acid composition of the dry seed was determined by gas chromatography. Noni seeds are albuminous and have a thick seed coat, rich in lignin. Lipid and protein bodies were observed inside the endosperm cells, representing 43.50% and 9.15% respectively, while the reservoir of soluble sugars was less than 5%. Linoleic acid was the most prevalent with 68.1%. The lipids were mobilized during germination, suffering a reduction of up to 38% of its total. Proteins, as well as lipids decreased by 25.78% during the germination period observed. The main reserves of noni seeds are lipids and proteins that are mobilized during germination to provide energy and matter to the developing embryo and synthesis of more complex compounds.

  6. Ethnoveterinary application of Morinda citrifolia fruit puree on a commercial heifer rearing facility with endemic salmonellosis.

    Science.gov (United States)

    Brooks, V J; De Wolfe, T J; Paulus, T J; Xu, J; Cai, J; Keuler, N S; Godbee, R G; Peek, S F; McGuirk, S M; Darien, B J

    2012-01-01

    We have previously reported that Morinda citrifolia (noni) puree modulates neonatal calves developmental maturation of the innate and adaptive immune system. In this study, the effect of noni puree on respiratory and gastrointestinal (GI), health in preweaned dairy calves on a farm with endemic salmonellosis was examined. Two clinical trials were conducted whereby each trial evaluated one processing technique of noni puree. Trials 1 and 2 tested noni versions A and B, respectively. Puree analysis and trial methods were identical to each other, with the calf as the experimental unit. Calves were designated to 1 of 3 treatment groups in each trial and received either: 0, 15 or 30 mL every 12 hr of noni supplement for the first 3 weeks of life. Health scores, weaning age, weight gain from admission to weaning, and weaned by 6 weeks, were used as clinical endpoints for statistical analysis. In trial 1, calves supplemented with 15 mL noni puree of version A every 12 hr had a higher probability of being weaned by 6 weeks of age than control calves (P = 0.04). In trial 2, calves receiving 30 mL of version B every 12 hr had a 54.5% reduction in total medical treatments by 42 days of age when compared to controls (P = 0.02). There was a trend in reduced respiratory (61%), and GI (52%) medical treatments per calf when compared to controls (P = 0.06 and 0.08, respectively). There were no differences in weight gain or mortality for any treatment group in either trial.

  7. Anticancer Effects of Extracts from the Fruit of Morinda Citrifolia (Noni) in Breast Cancer Cell Lines.

    Science.gov (United States)

    Sharma, K; Pachauri, S D; Khandelwal, K; Ahmad, H; Arya, A; Biala, P; Agrawal, S; Pandey, R R; Srivastava, A; Srivastav, A; Saxena, J K; Dwivedi, A K

    2016-03-01

    Morinda citrifolia L. (NONI) fruits have been used for thousands of years for the treatment of many health problems including cancer, cold, diabetes, flu, hypertension, and pain. Plant extracts have reported several therapeutic benefits, but extraction of individual compound from the extract often exhibits limited clinical utility as the synergistic effect of various natural ingredients gets lost. They generally constitute polyphenols and flavonoids. Studies have suggested that these phytochemicals, especially polyphenols, display high antioxidant properties, which help to reduce the risk of degenerative diseases, such as cancer and cardiovascular diseases. Several in-vitro and in-vivo studies have shown that Noni fruits have antioxidant, anti-inflammatory, anti-dementia, liver-protective, anticancer, analgesic, and immunomodulatory effects. Till date about 7 in vitro cancer studies have been done, but a detailed in vitro study including cell cycle and caspase activation assay on breast cancer cell line has not been done. In the present study different Noni fruit fractions have tested on cancer cell lines MCF-7, MDA-MB-231 (breast adenocarcinoma) and one non-cancer cell line HEK-293 (Human embryonic kidney). Out of which ethylacetate extract showed a higher order of in vitro anticancer activity profile. The ethylacetate extract strongly inhibited the proliferation of MCF-7, MDA-MB-231 and HEK-293 cell lines with IC50 values of 25, 35, 60 µg/ml respectively. The extract showed increase in apoptotic cells in MCF-7 and MDA-MB-231 cells and arrested the cell cycle in the G1/S phase in MCF-7 and G0/G1 phase in MDA-MB-231 cells. Noni extract also decreases the intracellular ROS generation and mitochondrial membrane potential.

  8. Antibacterial Activity of Ethyl Acetate the Extract of Noni Fruit (Morinda citrifolia L.) Against Bacterial Spoilage in Fish

    Science.gov (United States)

    Nugraheni, E. R.; Adriani, G. R.; Munawaroh, H.

    2017-04-01

    Noni fruit (Morinda citrifolia L.) contains compounds that have potential as antibacterial agent. Antibacterial compounds produced noni fruit (M. citrifolia L.) can inhibit bacterial growth. This study was conducted to test the antibacterial activity of ethyl acetate extract of noni fruit (M. citrifolia L.) against spoilage bacterial in fish. Pseudomonas aeruginosa, Bacillus cereus, Escherichia coli, Klebsiella oxytoca, and Enterobacter aerogenes isolates and examine antibacterial phytochemical profile. Extraction of noni compounds was done by maceration, followed by partition with ethyl acetate to obtain the soluble and insoluble ethyl acetate fraction. Previews result show that the ethyl acetate extract had very strong activity. Extraction process continued by separation and isolation used preparative thin layer chromatography method, so that obtained five isolates and mark them as A, B, C, D and E. Antibacterial activity assay performed on isolates A, B, C, D, and E with 20 and 30% concentration. The test results showed that isolates A could not be inhibit the growth of bacteria, isolates B, C, D, and E has antibacterial activity with weak to strong inhibition. Isolate B had the greatest inhibition activity against the B. cereus, whereas isolates E had the greatest inhibition activity against P. aeroginosa. MIC (Minimum Inhibitor Concentration) and MBC (Minimum Bactericidal Concentration) test result showed that MIC and MBC values could not be determined. Analysis of compounds by TLC showed that isolate B suspected contains coumarin or flavonoids compounds that have antibacterial activity.

  9. Effect of Morinda citrifolia leaf as saponin sources on fermentation characteristic, protozoa defaunated, gas and methane production of ruminal fluid in vitro

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    Hendra Herdian

    2011-06-01

    Full Text Available Many studies have reported that the Morinda citrifolia (pace plant was a useful material for human health. However the exploration of this plant on rumen fermentation is still needed. Therefore, a research was done to study the effect of M. citrifolia leaf on fermentation characteristics of rumen fluid consisted of protozoa defaunated process, VFA composition, NH3 content, rumen microbial protein content, gas and methane production using in vitro techniques. Rumen fluid obtained from two fistulated Ongole crossbreed cattle fed with forage and concentrate feed ration (70 : 30. The fluid was incubated at 39ºC for 48 hours. The treatment on the rumen fluid consisted of control treatment: 100% (200 mg DM kolonjono forage substrate (Penisetum purpureum and M. citrifolia treatments: kolonjono forage plus M. citrifolia (equivalent saponin 3; 6; 9; and 12 mg DM, respectively. The treatment of M. citrifolia leaf addition showed declined patterns in the number of protozoa population (P 0.05. Microbial protein content in rumen fluid increased (P 0.05 compared to control, while M. citrifolia treatments reduced the methane gas production of (P < 0.05 compared to control. It was concluded that M. citrifolia leaf has potential as a limiting agent of protozoa population and methane gas production in rumen.

  10. Atividade antioxidante da polpa, casca e sementes do noni (Morinda citrifolia Linn

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    Adriana Barbosa Costa

    2013-06-01

    Full Text Available Na busca pela identificação de novas fontes de antioxidantes naturais e de esclarecer lacunas acerca das reais propriedades benéficas atribuídas ao Noni (Morinda citrifolia Linn, este trabalho teve como objetivo realizar a caracterização química e avaliar a atividade antioxidante da polpa, casca e sementes do noni. Foram determinadas a composição centesimal (umidade, cinzas, proteínas, carboidratos e lipídios; os compostos bioativos (fenólicos totais, carotenoides totais e vitamina C e a atividade in vitro em extratos aquoso, etanólico e acetônico. Os resultados demonstraram que o Noni possui quantidades significativas de carboidratos (27,21%; 9,70% e 8,37% e de proteínas (2,64%; 2,23%; e 2,24% nas sementes, casca e polpa, respectivamente. A polpa apresentou maior teor de vitamina C (23,1 mg/100g e de carotenoides totais (3,90 mg/100g. No extrato acetônico da polpa, foram quantificados 109,81 mg/100g de fenólicos totais, seguidos pelos extratos acetônicos da casca (76,01 mg/100g, das sementes (28,75 mg/100g e do extrato etanólico da polpa (20,33 mg/100g. Todos os extratos avaliados apresentaram atividade antioxidante in vitro; os extratos acetônico e etanólico da casca e das sementes do Noni apresentaram maior atividade pelo método β-caroteno/ ácido linoleico, enquanto o extrato etanólico da polpa teve maior atividade antioxidante pelo ensaio DPPH e ABTS, e o extrato acetônico da polpa, pelo método ABTS. O noni é um fruto com significativo teor de compostos fenólicos totais que apresentam atividade antioxidante in vitro.

  11. Polysaccharide-Rich Fraction of Noni Fruit (Morinda citrifolia L.) as Doxorubicin Co-Chemotherapy: Evaluation of Catalase, Macrophages, and TCD8+ Lymphocytes.

    Science.gov (United States)

    Sasmito, Ediati; Hertiani, Triana; Novlita Renggani, Tiya; Jaya Laksana, Brata

    2015-01-01

    Noni fruit (Morinda citrifolia L.) has been acknowledged for its cytotoxic and immunostimulatory activity. Our previous results on the immunomodulatory effect of a noni juice polysaccharide-rich fraction encouraged this research to evaluate the potency of the polysaccharide-rich fraction as co-chemotherapy with doxorubicin (DOX) administration. Macrophage activity (MA) was evaluated with the latex bead method. The phagocytic index (PI) was measured as the number of latex beads ingested by 100 macrophages, while the phagocytosis ratio (PR) was indicated by the percentage of macrophages that ingested three or more latex beads. The CEC was evaluated by using a commercial assay kit, while CD8+ T lymphocyte proliferation was evaluated using a flowcytometry method following in vivo administration. Thirty male Wistar rats were divided into five groups (n = 6 each). The control group received DOX via i.p. at a concentration of 4.67 mg/kg BW on days 1 and 4; four treatment groups received PF p.o. at a concentration of 25; 50; 100; 200 mg/kg BW daily, respectively, and additionally DOX i.p. 4.67 mg/kg BW (days 1 and 4) for 7 days. The phagocytic activity was not affected significantly by PF administration compared to the Dox control, but PF administration at a dose of 25 and 50 mg/kg BW has been proven to increase TCD8+ cell proliferation in combination with DOX. The catalase concentration, on the other hand, significantly decreased following PF administration at a dose of 100 mg/kg BW. The results suggest that the polysaccharide-rich fraction of noni juice might induce immunomodulatory effects via TCD8+ activation, have antioxidant activity, and thus might be a potential candidate to be used as an adjuvant to DOX chemotherapy.

  12. The Exotic Plants of Indonesia: Mahkota Dewa (Phaleria macrocarpa, Sikaduduak (Melastoma malabathricum Linn and Mengkudu (Morinda citrifolia as Potent Antioxidant Sources

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    Tuty Anggraini

    2015-04-01

    Full Text Available The present study aims to determine the antioxidant activity of of three unique plants from Indonesia used for medicine of dried and etahnolic extract. Plants that are commonly used as medicine is Phaleria macrocarpa known as mahkota dewa (In West Sumatra, Melastoma malabathricum known as sikaduduak and Morinda citrifolia known as mengkudu. The DPPH methods used to the antioxidant assay. The dried Melastoma malabathricum dried and ethanolic extract shows the highest antioxidant activity than Phaleria macrocarpa and Morinda citrifolia both powder  and ethanolic extract. Started from 0,125mg/L for PMm and 0,25mg/L for PPm can be used for antioxidant measurement with DPPH.

  13. Study Progress of Morinda citrifolia%诺丽研究进展(一)——国内外研究进展

    Institute of Scientific and Technical Information of China (English)

    李法营; 蓝增全; 刘昌芬; 李青红

    2009-01-01

    Based on the description of Noni's biotical and ecological characters, the study progress of the development and utilization, the propagation techniques, the introduction and cultivation of Morinda citrifolia at home and abroad were summarized, at the same time , pointed out that problems and deficiency in Morinda citrifolia research process, in order to provide theoretical foundation for the further utilization.%通过对诺丽植物学、生态学特征的描述,以及对诺丽开发利用、繁殖技术、引种栽培等方面的国内外研究进展进行了综合概述,同时指出了诺丽研究进程中发现的问题和不足,以期为进一步开发利用该植物提供理论依据.

  14. Noni (Morinda citrifolia L.) Fruit Extracts Improve Colon Microflora and Exert Anti-Inflammatory Activities in Caco-2 Cells.

    Science.gov (United States)

    Huang, Hsin-Lun; Liu, Cheng-Tzu; Chou, Ming-Chih; Ko, Chien-Hui; Wang, Chin-Kun

    2015-06-01

    Intestinal microflora and inflammation are associated with the risk of inflammatory bowel diseases. Noni (Morinda citrifolia L.) has various bioactivities, but its effect on colon health remains unknown. This study focused on the effects of fermented noni fruit extracts on colon microflora and inflammation of colon epithelial cells. The anti-inflammatory activities of ethanol and ethyl acetate extracts on Caco-2 cells were evaluated including interleukin-8 (IL-8) and cyclooxygenase-2 (COX-2). The growth of Lactobacillus and Bifidobacterium species was promoted by ethanol extract. Ethyl acetate extract decreased intracellular reactive oxygen species and significantly suppressed COX-2, IL-8, and prostaglandin E2 production and neutrophil chemotaxis by suppressing the translocation of the p65 subunit. Quercetin was the main contributor to the anti-inflammatory activity. The fermented noni fruit promoted probiotic growths and downregulated the intracellular oxidation and inflammation in Caco-2 cells. These results suggest that fermented noni fruit might protect against inflammatory diseases of the colon.

  15. Genetic Diversity Analysis of Morinda citrifolia by ISSR Markers%海巴戟ISSR遗传多样性分析

    Institute of Scientific and Technical Information of China (English)

    廖丹; 程江波; 聂风琴; 李明; 林道哲; 符文英

    2016-01-01

    The genetic diversity and cluster analysis of the 202 Morinda citrifolia germplasm resources were an-alyzed by using ISSR molecular markers.The results showed that 13 ISSR primers which amplified 65 loci,meaned the average of each primer could amplified five loci.Among them,63 bands (96.92%)were polymorphic.Cluster analysis result indicated that 202 germplasm resources could be clustered into five groups when the genetic similari-ty coefficient was 0.63,revealedthe characteristics of geographical distribution.The average of Shannon information index (I),Neis genetic diversity (H)and effective number of alleles (Ne)was 0.5206,0.3488 and 1.6042, respectively,which indicated there was highly genetic diversity in these 202 Morinda citrifolia resource .%利用ISSR分子标记对202份海巴戟种质材料进行遗传多样性和聚类分析。结果表明:13条ISSR引物共扩增出65个位点,每个引物平均扩增出5个位点,其中多态性位点63个,占总数的96.92%。在相似系数为0.63时,202份海巴戟种质分为5个类,表现出一定的地域分布规律。检测到有效等位基因数 Ne =1.6042,基因多样性 H=0.3488,Shannon′s信息指数 I=0.5206,说明202份海巴戟种质间存在着较高的遗传多样性。

  16. Effect of Noni Leaves Extract (Morinda citrifolia L. Supplementation in Feed on Physical Quality of Broiler Breast Meat

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    Aji Sukoco

    2015-08-01

    Full Text Available This research was conducted to know the effect of noni leaves extract (Morinda citrifolia L. supplementation in feed on physical quality of broiler breast meat such as pH, Water Holding Capacity (WHC, Cooking Loss (CL, and tenderness. Ninety six 8-days old broiler chickens strain Lohmann and of undifferentiated sex (unsexed were used in this research. The broiler chickens will be reared until 35-days old. The research method was experimental using Completely Randomized Design (CRD with six treatments and four replications, each replication consisted of four broiler chickens. The treatments consisted of P0 (Basal Feed, P1 (Basal Feed + tetracycline 0.05%, P2 (Basal Feed + noni leaves extract 0.05%, P3 (Basal feed + noni leaves extract 0.1%, P4 (Basal feed + noni leaves extract 0.15%, P5 (Basal feed + noni leaves extract 0.2%. The data were analyzed by ANOVA and continued by Least Significant Difference (LSD test if there was significantly different result. The results showed that noni leaves extract did not give significant effect (P>0.05 on meat pH, water holding capacity (WHC, cooking loss (CL, and tenderness. However, these results were still acceptable normally such as pH between 5.38-5.57, water holding capacity 34.13-45.64%, cooking loss 33.05-36.97%, but tenderness 16.22-20.57N were less acceptable. The research concluded that supplementation of noni leaves extract (Morinda citrifolia L. in feed did not increase physical quality of broiler breast meat on pH, Water Holding Capacity (WHC, Cooking Loss (CL, and tenderness.

  17. Peran Ekstrak Etanol Topikal Daun Mengkudu (Morinda citrifolia L.) pada Penyembuhan Luka Ditinjau dari Imunoekspresi CD34 dan Kolagen pada Tikus Galur Wistar

    OpenAIRE

    Indah Puti Rahmayani S; Ani Melani Maskoen; Bethy S. Hernowo

    2013-01-01

    Problems in wound healing occurred if proper care is not given and the wound develops into a chronic wound. Noni (Morinda citrifolia L.) is one of the most common plants in tropical areas, including Indonesia, which fruit, leaves dan root are used in traditional treatment, for example wound healing. This experimental research with post test-only control group design identified the effect of topical application of noni leaves ethanol extract on wound healing by examining the histopathological ...

  18. PENGARUH PENGGUNAAN TEPUNG SILASE DAUN MENGKUDU (Morinda citrifolia DALAM FORMULA PAKAN TERHADAP PERTUMBUHAN IKAN SIDAT (Anguilla bicolor STADIA ELVER

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    Dewi Cholifah

    2012-10-01

    Full Text Available Penelitian ini dilakukan untuk mengetahui pengaruh dan dosis terbaik penggunaan tepung silase daun Mengkudu (M. citrifolia dalam formula pakan terhadap pertumbuhan ikan Sidat (A. bicolor stadia elver. Penelitian dibagi menjadi dua tahap, tahap pertama formulasi pakan percobaan, dan tahap kedua uji in vivo/uji formula pakan percobaan skala laboratorium pada ikan Sidat. Pelaksanaan penelitian tahap 1 adalah membuat formula pakan iso protein (40% dan iso energi (3.600 kkal/kg pakan dengan memanfaatkan tepung silase daun Mengkudu sesuai perlakuan, menggunakan metode lembar kerja. Penelitian tahap 2 berdasarkan Rancangan Acak Lengkap (RAL dengan tiga ulangan. Empat perlakuan substitusi protein tepung silase daun Mengkudu terhadap protein tepung ikan yang berbeda, meliputi perlakuan A 0%(0:100; B 10%(10:90; C 20%(20:80 dan D 30%(30:70. Parameter utama adalah kelulushidupan (survival rate, laju pertumbuhan spesifik (specific growth rate, rasio konversi pakan (feed convertion ratio dan rasio efisiensi protein (protein efficiency ratio. Hasil penelitian menunjukkan bahwa penggunaan tepung silase daun Mengkudu dalam formula pakan berpengaruh nyata terhadap parameter laju pertumbuhan spesifik, rasio konversi pakan dan rasio efisiensi protein. Dosis terbaik tepung silase daun Mengkudu  mensubstitusi protein tepung ikan dalam formula pakan sebesar 14,71 – 15% yang menghasilkan nilai laju pertumbuhan spesifik 0,72 %BB/hari, rasio konversi pakan 3,38, dan rasio efisiensi protein 0,718.Kata kunci: formula pakan, silase daun Morinda citrifolia, Anguilla bicolor

  19. Melanogenesis-inhibitory saccharide fatty acid esters and other constituents of the fruits of Morinda citrifolia (noni).

    Science.gov (United States)

    Akihisa, Toshihiro; Tochizawa, Shun; Takahashi, Nami; Yamamoto, Ayako; Zhang, Jie; Kikuchi, Takashi; Fukatsu, Makoto; Tokuda, Harukuni; Suzuki, Nobutaka

    2012-06-01

    Five new saccharide fatty acid esters, named nonioside P (3), nonioside Q (4), nonioside R (8), nonioside S (10), and nonioside T (14), and one new succinic acid ester, butyl 2-hydroxysuccinate (=4-butoxy-3-hydroxy-4-oxobutanoic acid) (31), were isolated, along with 26 known compounds, including eight saccharide fatty acid esters, 1, 2, 5, 6, 7, 9, 12, and 13, three hemiterpene glycosides, 15, 17, and 18, six iridoid glycosides, 21-25, and 27, and nine other compounds, 20, 28, 29, and 32-37, from a MeOH extract of the fruit of Morinda citrifolia (noni). Upon evaluation of these and five other glycosidic compounds, 11, 16, 19, 26, and 30, from M. citrifolia fruit extract for their inhibitory activities against melanogenesis in B16 melanoma cells induced with α-melanocyte-stimulating hormone (α-MSH), most of the saccharide fatty acid esters, hemiterpene glycosides, and iridoid glycosides showed inhibitory effects with no or almost no toxicity to the cells. These compounds were further evaluated with respect to their cytotoxic activities against two human cancer cell lines (HL-60 and AZ521) and their inhibitory effects on Epstein-Barr virus early antigen (EBV-EA) activation induced with 12-O-tetradecanoylphorbol-13-acetate (TPA) in Raji cells.

  20. Regulation of glucose metabolism via hepatic forkhead transcription factor 1 (FoxO1) by Morinda citrifolia (noni) in high-fat diet-induced obese mice.

    Science.gov (United States)

    Nerurkar, Pratibha V; Nishioka, Adrienne; Eck, Philip O; Johns, Lisa M; Volper, Esther; Nerurkar, Vivek R

    2012-07-01

    Renewed interest in alternative medicine among diabetic individuals prompted us to investigate anti-diabetic effects of Morinda citrifolia (noni) in high-fat diet (HFD)-fed mice. Type 2 diabetes is associated with increased glucose production due to the inability of insulin to suppress hepatic gluconeogenesis and promote glycolysis. Insulin inhibits gluconeogenesis by modulating transcription factors such as forkhead box O (FoxO1). Based on microarray analysis data, we tested the hypothesis that fermented noni fruit juice (fNJ) improves glucose metabolism via FoxO1 phosphorylation. C57BL/6 male mice were fed a HFD and fNJ for 12 weeks. Body weights and food intake were monitored daily. FoxO1 expression was analysed by real-time PCR and Western blotting. Specificity of fNJ-associated FoxO1 regulation of gluconeogenesis was confirmed by small interfering RNA (siRNA) studies using human hepatoma cells, HepG2. Supplementation with fNJ inhibited weight gain and improved glucose and insulin tolerance and fasting glucose in HFD-fed mice. Hypoglycaemic properties of fNJ were associated with the inhibition of hepatic FoxO1 mRNA expression, with a concomitant increase in FoxO1 phosphorylation and nuclear expulsion of the proteins. Gluconeogenic genes, phosphoenolpyruvate C kinase (PEPCK) and glucose-6-phosphatase (G6P), were significantly inhibited in mice fed a HFD+fNJ. HepG2 cells demonstrated more than 80 % inhibition of PEPCK and G6P mRNA expression in cells treated with FoxO1 siRNA and fNJ. These data suggest that fNJ improves glucose metabolism via FoxO1 regulation in HFD-fed mice.

  1. Methanolic Extract of Morinda citrifolia L. (Noni) Unripe Fruit Attenuates Ethanol-Induced Conditioned Place Preferences in Mice.

    Science.gov (United States)

    Khan, Yasmin; Pandy, Vijayapandi

    2016-01-01

    Phytotherapy is an emerging field successfully utilized to treat various chronic diseases including alcohol dependence. In the present study, we examined the effect of the standardized methanolic extract of Morinda citrifolia Linn. unripe fruit (MMC), on compulsive ethanol-seeking behavior using the mouse conditioned place preference (CPP) test. CPP was established by injections of ethanol (2 g/kg, i.p.) in a 12-day conditioning schedule in mice. The effect of MMC and the reference drug, acamprosate (ACAM), on the reinforcing properties of ethanol in mice was studied by the oral administration of MMC (1, 3, and 5 g/kg) and ACAM (300 mg/kg) 60 min prior to the final CPP test postconditioning. Furthermore, CPPs weakened with repeated testing in the absence of ethanol over the next 12 days (extinction), during which the treatment groups received MMC (1, 3, and 5 g/kg, p.o.) or ACAM (300 mg/kg, p.o.). Finally, a priming injection of a low dose of ethanol (0.4 g/kg, i.p.) in the home cage (Reinstatement) was sufficient to reinstate CPPs, an effect that was challenged by the administration of MMC or ACAM. MMC (3 and 5 g/kg, p.o.) and ACAM (300 mg/kg, p.o.) significantly reversed the establishment of ethanol-induced CPPs and effectively facilitated the extinction of ethanol CPP. In light of these findings, it has been suggested that M. citrifolia unripe fruit could be utilized for novel drug development to combat alcohol dependence.

  2. Methanolic extract of Morinda citrifolia L. (noni unripe fruit attenuates ethanol-induced conditioned place preferences in mice

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    Yasmin Khan

    2016-09-01

    Full Text Available Phytotherapy is an emerging field successfully utilized to treat various chronic diseases including alcohol dependence. In the present study, we examined the effect of the standardized methanolic extract of Morinda citrifolia Linn. unripe fruit (MMC, on compulsive ethanol-seeking behaviour using the mouse conditioned place preference (CPP test. CPP was established by injections of ethanol (2g/kg, i.p. in a 12-day conditioning schedule in mice. The effect of MMC and the reference drug, acamprosate (ACAM, on the reinforcing properties of ethanol in mice was studied by the oral administration of MMC (1, 3 and 5g/kg and ACAM (300 mg/kg 60 min prior to the final CPP test postconditioning. Furthermore, CPPs weakened with repeated testing in the absence of ethanol over the next 12 days (extinction, during which the treatment groups received MMC (1, 3 and 5g/kg, p.o. or ACAM (300 mg/kg, p.o.. Finally, a priming injection of a low dose of ethanol (0.4g/kg, i.p. in the home cage (Reinstatement was sufficient to reinstate CPPs, an effect that was challenged by the administration of MMC or ACAM. MMC (3 and 5g/kg, p.o and ACAM (300 mg/kg, p.o. significantly reversed the establishment of ethanol-induced CPPs and effectively facilitated the extinction of ethanol CPP. In light of these findings, it has been suggested that M. citrifolia unripe fruit could be utilized for novel drug development to combat alcohol dependence.

  3. Antiviral activity of extracts from Morinda citrifolia leaves and chlorophyll catabolites, pheophorbide a and pyropheophorbide a, against hepatitis C virus.

    Science.gov (United States)

    Ratnoglik, Suratno Lulut; Aoki, Chie; Sudarmono, Pratiwi; Komoto, Mari; Deng, Lin; Shoji, Ikuo; Fuchino, Hiroyuki; Kawahara, Nobuo; Hotta, Hak

    2014-03-01

    The development of complementary and/or alternative drugs for treatment of hepatitis C virus (HCV) infection is still needed. Antiviral compounds in medicinal plants are potentially good targets to study. Morinda citrifolia is a common plant distributed widely in Indo-Pacific region; its fruits and leaves are food sources and are also used as a treatment in traditional medicine. In this study, using a HCV cell culture system, it was demonstrated that a methanol extract, its n-hexane, and ethyl acetate fractions from M. citrifolia leaves possess anti-HCV activities with 50%-inhibitory concentrations (IC(50)) of 20.6, 6.1, and 6.6 μg/mL, respectively. Bioactivity-guided purification and structural analysis led to isolation and identification of pheophorbide a, the major catabolite of chlorophyll a, as an anti-HCV compound present in the extracts (IC(50) = 0.3 μg/mL). It was also found that pyropheophorbide a possesses anti-HCV activity (IC(50) = 0.2 μg/mL). The 50%-cytotoxic concentrations (CC(50)) of pheophorbide a and pyropheophorbide a were 10.0 and 7.2 μg/mL, respectively, their selectivity indexes being 33 and 36, respectively. On the other hand, chlorophyll a, sodium copper chlorophyllin, and pheophytin a barely, or only marginally, exhibited anti-HCV activities. Time-of-addition analysis revealed that pheophorbide a and pyropheophorbide a act at both entry and the post-entry steps. The present results suggest that pheophorbide a and its related compounds would be good candidates for seed compounds for developing antivirals against HCV.

  4. Experiment on Tissue Culture of Morinda citrifolia%海滨木巴戟组织培养研究

    Institute of Scientific and Technical Information of China (English)

    郭佳; 朱鹏飞; 房丹; 高中海; 徐梁

    2013-01-01

    The axillary buds of Morinda citrifolia were used as the explants to establish the micro-propagation system. The orthogonal experiment of bud differentiation was carried out by TDZ, BA and IBA. The results showed that three hormones had no significant(p < 0.05) effect on bud differentiation, but IBA inhibited the differentiation. The number of vitrification shoots increased with the concentration of BA when the medium was only added BA. Experiment on rooting induction demonstrated that the influences of NAA and IBA had no obvious difference (p<0.05). However, more callus were induced from the base of explant in the medium with IBA.%以海滨木巴戟(Morinda citrifolia)的嫩芽为外植体进行组培研究,以1/2MS+蔗糖30 g/L+琼脂6 g/L为基本培养基,以TDZ、BA、IBA三种激素进行不定芽的诱导分化实验,以IBA、NAA进行生根实验。结果表明,各因素对外植体分化的影响皆不显著(p<0.05),IBA抑制外植体分化;在只添加BA的培养基中,随着BA浓度的升高,外植体玻璃化的现象有增加的趋势;生根实验结果表明,NAA和IBA诱导生根效果相差不大(p<0.05),但是IBA在外植体基部诱导出更多的愈伤组织。

  5. Morinda citrifolia L. fruit extracts modulates H2O2-induced oxidative stress in human liposarcoma SW872 cells.

    Science.gov (United States)

    Ruhomally, Z; Somanah, J; Bahorun, T; Neergheen-Bhujun, V S

    2016-07-01

    Morinda citrifolia L. commonly known as noni is used by the pharmaceutical and cosmetic industries due to the plethora of pharmacological activities of its metabolites. In Mauritius, the fruits of M. citrifolia are used in folk medicine against a number of indications. The present study aimed at evaluating the antioxidant activity of ripe and unripe noni fruit at both biochemical and cellular levels. Using an array of established assay systems, the fruit antioxidant propensity was assessed in terms of its radical scavenging, iron reducing and metal chelating potentials. Ascorbic acid, total phenolic and total flavonoid contents of the fruits were also determined. The ascorbic acid content of ripe noni was 76.24 ± 1.13 mg/100 g while total phenolics of ripe and unripe fruit extracts were 748.40 ± 8.85 μg and 770.34 ± 2.27 μg GAE g(-1) FW respectively. Both the ripe and unripe extracts of M. citrifolia were potent scavengers of nitric oxide, superoxide and hydroxyl radicals. The ferric reducing capacity ranged from 11.26 ± 0.33 to 11.90 ± 0.20 mM Fe(2+) g(-1) FW while the IC50 values for the iron (II) chelating power were 0.50 ± 0.01 and 1.74 ± 0.01 g FW/mL for the ripe and unripe fruit extracts respectively. Cellular studies additionally demonstrated that noni were able to dose-dependently counteract accumulation of reactive oxygen species (ROS)-induced oxidative stress, a potential obesogenic factor within human liposarcoma SW872 cells as well as significantly restore cell death within the concentration range of 0.106-0.813 g/mL. Results reported herein suggest noni as an interesting source of prophylactic antioxidants modulated by its polyphenol composition.

  6. Larvicidal activity of Morinda citrifolia L. (Noni) (Family: Rubiaceae) leaf extract against Anopheles stephensi, Culex quinquefasciatus, and Aedes aegypti.

    Science.gov (United States)

    Kovendan, Kalimuthu; Murugan, Kadarkarai; Shanthakumar, Shanmugam Perumal; Vincent, Savariar; Hwang, Jiang-Shiou

    2012-10-01

    Morinda citrifolia leaf extract was tested for larvicidal activity against three medically important mosquito vectors such as malarial vector Anopheles stephensi, dengue vector Aedes aegypti, and filarial vector Culex quinquefasciatus. The plant material was shade dried at room temperature and powdered coarsely. From the leaf, 1-kg powder was macerated with 3.0 L of hexane, chloroform, acetone, methanol, and water sequentially for a period of 72 h each and filtered. The yield of extracts was hexane (13.56 g), chloroform (15.21 g), acetone (12.85 g), methanol (14.76 g), and water (12.92 g), respectively. The extracts were concentrated at reduced temperature on a rotary vacuum evaporator and stored at a temperature of 4°C. The M. citrifolia leaf extract at 200, 300, 400, 500, and 600 ppm caused a significant mortality of three mosquito species. Hexane, chloroform, acetone, and water caused moderate considerable mortality; however, the highest larval mortality was methanolic extract, observed in three mosquito vectors. The larval mortality was observed after 24-h exposure. No mortality was observed in the control. The third larvae of Anopheles stephensi had values of LC(50) = 345.10, 324.26, 299.97, 261.96, and 284.59 ppm and LC(90) = 653.00, 626.58, 571.89, 505.06, and 549.51 ppm, respectively. The Aedes aegypti had values of LC(50) = 361.75, 343.22, 315.40, 277.92, and 306.98 ppm and LC(90) = 687.39, 659.02, 611.35, 568.18, and 613.25 ppm, respectively. The Culex quinquefasciatus had values of LC(50) = 382.96, 369.85, 344.34, 330.42, and 324.64 ppm and LC(90) = 726.18, 706.57, 669.28, 619.63, and 644.47 ppm, respectively. The results of the leaf extract of M. citrifolia are promising as good larvicidal activity against the mosquito vector Anopheles stephensi, Aedes aegypti, and Culex quinquefasciatus. This is a new eco-friendly approach for the control of vector control programs. Therefore, this study provides first report on the larvicidal activities against three

  7. Chrysin, Abundant in Morinda citrifolia Fruit Water-EtOAc Extracts, Combined with Apigenin Synergistically Induced Apoptosis and Inhibited Migration in Human Breast and Liver Cancer Cells.

    Science.gov (United States)

    Huang, Cheng; Wei, Yu-Xuan; Shen, Ma-Ching; Tu, Yu-Hsuan; Wang, Chia-Chi; Huang, Hsiu-Chen

    2016-06-01

    The composition of Morinda citrifolia (M. citrifolia) was determined using high-performance liquid chromatography (HPLC), and the anticancer effects of M. citrifolia extract evaluated in HepG2, Huh7, and MDA-MB-231 cancer cells. M. citrifolia fruit extracts were obtained by using five different organic solvents, including hexane (Hex), methanol (MeOH), ethyl acetate (EtOAc), chloroform (CHCl3), and ethanol (EtOH). The water-EtOAc extracts from M. citrifolia fruits was found to have the highest anticancer activity. HPLC data revealed the predominance of chrysin in water-EtOAc extracts of M. citrifolia fruit. Furthermore, the combined effects of cotreatment with apigenin and chrysin on liver and breast cancer were investigated. Treatment with apigenin plus chrysin for 72-96 h reduced HepG2 and MDA-MB-231 cell viability and induced apoptosis through down-regulation of S-phase kinase-associated protein-2 (Skp2) and low-density lipoprotein receptor-related protein 6 (LRP6) expression. However, the combination treatment for 36 h synergistically decreased MDA-MB-231 cell motility but not cell viability through down-regulation of MMP2, MMP9, fibronectin, and snail in MDA-MB-231 cells. Additionally, chrysin combined with apigenin also suppressed tumor growth in human MDA-MB-231 breast cancer cells xenograft through down-regulation of ki-67 and Skp2 protein. The experimental results showed that chrysin combined with apigenin can reduce HepG2 and MDA-MB-231 proliferation and cell motility and induce apoptosis. It also offers opportunities for exploring new drug targets, and further investigations are underway in this regard.

  8. Morinda citrifolia L.(noni) and memantine attenuate periventricular tissue injury of the fourth ventricle in hydrocephalic rabbits

    Institute of Scientific and Technical Information of China (English)

    Sibel K(o)ktürk; Süreyya Ceylan; Volkan Etus; Nezih Yasa; Sava(s) Ceylan

    2013-01-01

    This study was designed to evaluate the neuroprotective effects of Morinda citrifolia L. (Rubiaceae), commonly known as noni, and memantine (a N-methy-D-aspartate receptor inhibitor) on hydrocephalus-induced neurodegenerative disorders. Kaolin was injected into the cistern magna of male adult New Zealand rabbits to establish a hydrocephalus animal model. Memantine (20 mg/kg, intraperitoneally; memantine-treated group) or noni (5 mL/kg, intragastrically; noni-treated group) was administered daily for 2 weeks. Microtubule-associated protein-2 and caspase-3 immunohistochemistry were performed to detect neuronal degeneration and apoptosis in the periventricular tissue of the fourth ventricle of rabbits. Microtubule-associated protein-2 staining density was significantly decreased in the hydrocephalic group, while the staining density was significantly increased in the memantine- and noni-treated groups, especially in the noni-treated group. Noni treatment decreased the number of caspase-3-positive cells in rabbits with hydrocephalus, while memantine had no effect. These findings suggest that noni exhibits more obvious inhibitory effects on hydrocephalus-induced neurodegenerative disorders than memantine in periventricular tissue of the fourth ventricle.

  9. ATIVIDADE ANTIMICROBIANA E EFEITO COMBINADO SOBRE DROGAS ANTIFÚNGICAS E ANTIBACTERIANAS DO FRUTO DE Morinda citrifolia L.

    Directory of Open Access Journals (Sweden)

    Saulo R. TINTINO

    2015-01-01

    Full Text Available La aparición de microorganismos resistentes y la toxicidad asociada a los antimicrobianos aumenta la necesidad de investigar nuevos principios activos. Morinda citrifolia L., es una planta fructífera utilizada popularmente como antibacteriano y con otros diversos usos farmacológicos. Posee diversos metabolitos primarios y secundarios, principalmente flavonoides, triterpenoides y alcaloides. Este estudio tuvo como objetivo ensayar la potencial actividad antimicrobiana y moduladora de los extractos obtenidos de su fruta. Los extractos de las frutas fueron testados tanto para determinar el efecto antimicrobiano como también utilizándolo en combinación con agentes antibacterianos y antifúngicos contra microorganismos patógenos, por el método de microdilución en caldo. Se observó que la asociación entre antibióticos y extractos dió resultados clínicamente significativos en pruebas con bacterias Pseudomonas aeruginosa y Escherichia coli.

  10. Protein tyrosine phosphatase 1B (PTP1B) inhibitors from Morinda citrifolia (Noni) and their insulin mimetic activity.

    Science.gov (United States)

    Nguyen, Phi-Hung; Yang, Jun-Li; Uddin, Mohammad N; Park, So-Lim; Lim, Seong-Il; Jung, Da-Woon; Williams, Darren R; Oh, Won-Keun

    2013-11-22

    As part of our ongoing search for new antidiabetic agents from medicinal plants, we found that a methanol extract of Morinda citrifolia showed potential stimulatory effects on glucose uptake in 3T3-L1 adipocyte cells. Bioassay-guided fractionation of this active extract yielded two new lignans (1 and 2) and three new neolignans (9, 10, and 14), as well as 10 known compounds (3-8, 11-13, and 15). The absolute configurations of compounds 9, 10, and 14 were determined by ECD spectra analysis. Compounds 3, 6, 7, and 15 showed inhibitory effects on PTP1B enzyme with IC50 values of 21.86 ± 0.48, 15.01 ± 0.20, 16.82 ± 0.42, and 4.12 ± 0.09 μM, respectively. Furthermore, compounds 3, 6, 7, and 15 showed strong stimulatory effects on 2-NBDG uptake in 3T3-L1 adipocyte cells. This study indicated the potential of compounds 3, 6, 7, and 15 as lead molecules for antidiabetic agents.

  11. Antiadhesion and anti-inflammation effects of noni (Morinda citrifolia) fruit extracts on AGS cells during Helicobacter pylori infection.

    Science.gov (United States)

    Huang, Hsin-Lun; Ko, Chien-Hui; Yan, Yeong-Yu; Wang, Chin-Kun

    2014-03-19

    Helicobacter pylori is a human gastric pathogen that adheres to host cells and injects cytotoxin-associated gene A (CagA) to induce interleukin-8 (IL-8), inducible nitric oxide (iNOS), and cyclooxygenase 2 (COX-2). Noni (Morinda citrifolia) is found to possess antibacteria, anti-inflammation, and antioxidation activities, but its effect on H. pylori infection is still unknown. Ethanol and ethyl acetate extracts of noni fruit were used in this study. The inhibitory effect on CagA and H. pylori-induced IL-8, iNOS, and COX-2 were determined. The coculture medium was collected for measuring neutrophil chemotaxis. Both extracts of noni fruit showed weak inhibition on H. pylori. Both ethanol and ethyl acetate extracts provided antiadhesion of H. pylori to AGS cells and down-regulation on the CagA, IL-8, COX-2, and iNOS expressions. Results also indicated both extracts relieved neutrophil chemotaxis. Noni fruit extracts down-regulated inflammatory responses during H. pylori infection, and the phenolic compounds play key role in antiadhesion.

  12. Morinda citrifolia L. (noni) and memantine attenuate periventricular tissue injury of the fourth ventricle in hydrocephalic rabbits.

    Science.gov (United States)

    Köktürk, Sibel; Ceylan, Süreyya; Etus, Volkan; Yasa, Nezih; Ceylan, Savaş

    2013-03-25

    This study was designed to evaluate the neuroprotective effects of Morinda citrifolia L. (Rubiaceae), commonly known as noni, and memantine (a N-methy-D-aspartate receptor inhibitor) on hydrocephalus-induced neurodegenerative disorders. Kaolin was injected into the cistern magna of male adult New Zealand rabbits to establish a hydrocephalus animal model. Memantine (20 mg/kg, intraperitoneally; memantine-treated group) or noni (5 mL/kg, intragastrically; noni-treated group) was administered daily for 2 weeks. Microtubule-associated protein-2 and caspase-3 immunohistochemistry were performed to detect neuronal degeneration and apoptosis in the periventricular tissue of the fourth ventricle of rabbits. Microtubule-associated protein-2 staining density was significantly decreased in the hydrocephalic group, while the staining density was significantly increased in the memantine- and noni-treated groups, especially in the noni-treated group. Noni treatment decreased the number of caspase-3-positive cells in rabbits with hydrocephalus, while memantine had no effect. These findings suggest that noni exhibits more obvious inhibitory effects on hydrocephalus-induced neurodegenerative disorders than memantine in periventricular tissue of the fourth ventricle.

  13. Enhancement of anthraquinone production in Morinda citrifolia cell suspension cultures after stimulation of the proline cycle with two proline analogs.

    Science.gov (United States)

    Quevedo, Carla V; Perassolo, María; Giulietti, Ana M; Rodríguez Talou, Julián

    2012-03-01

    Synthesis of anthraquinones (AQs) involves the shikimate and 2-C-methyl-D-erythritol 4-phosphate pathways. The proline cycle is linked to the pentose phosphate pathway (PPP) to generate NADPH needed in the first steps of this pathway. The effect of two proline analogs, azetidine-2-carboxylic acid (A2C) and thiazolidine-4-carboxylic acid (T4C), were evaluated in Morinda citrifolia suspension cultures. Both analogs gave higher proline accumulation after 6 and 10 days (68 and 179% after 6 days with A2C at 25 and 50 μM, respectively, and 111% with T4C added at 100 μM). Induction of the proline cycle increased the AQ content after 6 days (~40% for 50 μM A2C and 100 μM T4C). Whereas A2C (50 μM) increased only AQ production, T4C also enhanced total phenolics. However, no induction of the PPP was observed with any of the treatments. This pathway therefore does not limit the supply of carbon skeletons to secondary metabolic pathways.

  14. Phyto-synthesis of silver nanoscale particles using Morinda citrifolia L. and its inhibitory activity against human pathogens.

    Science.gov (United States)

    Sathishkumar, Gnanasekar; Gobinath, Chandrakasan; Karpagam, Karuppiah; Hemamalini, Vedagiri; Premkumar, Kumpati; Sivaramakrishnan, Sivaperumal

    2012-06-15

    Leaf extract of Morinda citrifolia L. was assessed for the synthesis of silver nanoscale particles under different temperature and reaction time. Synthesized nanoscale (MCAgNPs) particles were confirmed by analysing the excitation of surface plasmon resonance (SPR) using UV-visible spectrophotometer at 420 nm. Further SEM, HRTEM analysis confirmed the range of particle size between 10 and 60 nm and SEAD pattern authorizes the face centered cubic (fcc) crystalline nature of the MCAgNPs. Fourier transform infrared spectrum (FTIR) of synthesized MCAgNPs confirms the presence of high amount of phenolic compounds in the plant extract which may possibly influence the reduction process and stabilization of nanoparticles. Further, inhibitory activity of MCAgNPs and plant extract were tested against human pathogens like Eschericia coli, Pseudomonas aeroginosa, Klebsiella pneumoniae, Enterobacter aerogenes, Bacillus cereus and Enterococci sp. The results indicated that the MCAgNPs showed moderate inhibitory actions against human pathogens than crude plant extract, demonstrating its antimicrobial value against pathogenic diseases.

  15. Matrix metalloproteinase-1 inhibitory activities of Morinda citrifolia seed extract and its constituents in UVA-irradiated human dermal fibroblasts.

    Science.gov (United States)

    Masuda, Megumi; Murata, Kazuya; Naruto, Shunsuke; Uwaya, Akemi; Isami, Fumiyuki; Matsuda, Hideaki

    2012-01-01

    The objective of this study was to examine whether a 50% ethanolic extract (MCS-ext) of the seeds of Morinda citrifolia (noni) and its constituents have matrix metalloproteinase-1 (MMP-1) inhibitory activity in UVA-irradiated normal human dermal fibroblasts (NHDFs). The MCS-ext (10 μg/mL) inhibited MMP-1 secretion from UVA-irradiated NHDFs, without cytotoxic effects, at 48 h after UV exposure. The ethyl acetate-soluble fraction of MCS-ext was the most potent inhibitor of MMP-1 secretion. Among the constituents of the fraction, a lignan, 3,3'-bisdemethylpinoresinol (1), inhibited the MMP-1 secretion at a concentration of 0.3 μM without cytotoxic effects. Furthermore, 1 (0.3 μM) reduced the level of intracellular MMP-1 expression. Other constituents, namely americanin A (2), quercetin (3) and ursolic acid (4), were inactive. To elucidate inhibition mechanisms of MMP-1 expression and secretion, the effect of 1 on mitogen-activated protein kinases (MAPKs) phosphorylation was examined. Western blot analysis revealed that 1 (0.3 μM) reduced the phosphorylations of p38 and c-Jun-N-terminal kinase (JNK). These results suggested that 1 suppresses intracellular MMP-1 expression, and consequent secretion from UVA-irradiated NHDFs, by down-regulation of MAPKs phosphorylation.

  16. Inhibitory effects of Morinda citrifolia extract and its constituents on melanogenesis in murine B16 melanoma cells.

    Science.gov (United States)

    Masuda, Megumi; Itoh, Kimihisa; Murata, Kazuya; Naruto, Shunsuke; Uwaya, Akemi; Isami, Fumiyuki; Matsuda, Hideaki

    2012-01-01

    The objective of this study was to examine the effects of Morinda citrifolia (noni) extract and its constituents on α-melanocyte stimulating hormone (α-MSH)-stimulated melanogenesis in cultured murine B16 melanoma cells (B16 cells). A 50% ethanolic extract of noni seeds (MCS-ext) showed significant inhibition of melanogenesis with no effect on cell proliferation. MCS-ext was more active than noni leaf and fruit flesh extracts. Activity guided fractionation of MCS-ext led to the isolation of two lignans, 3,3'-bisdemethylpinoresinol (1) and americanin A (2), as active constituents. To elucidate the mechanism of melanogenesis inhibition by the lignans, α-MSH-stimulated B16 cells were treated with 1 (5 μM) and 2 (200 μM). Time-dependent increases of intracellular melanin content and tyrosinase activity, during 24 to 72 h, were inhibited significantly by treatment with the lignans. The activity of 1 was greater than that of 2. Western blot analysis suggested that the lignans inhibited melanogenesis by down regulation of the levels of phosphorylation of p38 mitogen-activated protein kinase, resulting in suppression of tyrosinase expression.

  17. Isolation and structure determination of a benzofuran and a bis-nor-isoprenoid from Aspergillus niger grown on the water soluble fraction of Morinda citrifolia Linn. leaves.

    Science.gov (United States)

    Siddiqui, Bina S; Ismail, Fouzia A Sattar; Gulzar, Tahsin; Begum, Sabira

    2003-10-01

    The leaves of Morinda citrifolia, Linn. afforded a new benzofuran and a bis-nor-isoprenoid, blumenol C, hitherto unreported from this source. The structures of these have been elucidated as 5-benzofuran carboxylic acid-6-formyl methyl ester (1) and 4-(3'(R)-hydroxybutyl)-3,5,5, trimethyl-cyclohex-2-en-1-one (2) respectively through spectroscopic studies. The NMR data (including 1D, 2D techniques) and stereochemistry at C-3' of Compound 2 is also being reported for the first time.

  18. 海巴戟的组织培养及快速繁殖%Tissue Culture and Rapid Propagation of Morinda citrifolia L.

    Institute of Scientific and Technical Information of China (English)

    韦丽君; 吕平; 苏文潘; 俞奔驰; 叶启腾

    2006-01-01

    @@ 1植物名称海巴戟(Morinda citrifolia L.). 2材料类别从美国加里福尼亚叶启恩先生处得到大溪种海巴戟种,擦伤外壳后播种于砂盆中,可比未用粗砂擦伤外壳的提前4个月发芽(后者为6个月)植株长出2片完全叶时,取茎段和项芽作实验材料.

  19. Amelioration of apoptotic events in the skeletal muscle of intra-nigrally rotenone-infused Parkinsonian rats by Morinda citrifolia--up-regulation of Bcl-2 and blockage of cytochrome c release.

    Science.gov (United States)

    Narasimhan, Kishore Kumar S; Paul, Liya; Sathyamoorthy, Yogesh Kanna; Srinivasan, Ashokkumar; Chakrapani, Lakshmi Narasimhan; Singh, Abhilasha; Ravi, Divya Bhavani; Krishnan, Thulasi Raman; Velusamy, Prema; Kaliappan, Kathiravan; Radhakrishnan, Rameshkumar; Periandavan, Kalaiselvi

    2016-02-01

    Parkinson's disease is a progressive neurodegenerative movement disorder with the cardinal symptoms of bradykinesia, resting tremor, rigidity, and postural instability, which lead to abnormal movements and lack of activity, which in turn cause muscular damage. Even though studies have been carried out to elucidate the causative factors that lead to muscular damage in Parkinson's disease, apoptotic events that occur in the skeletal muscle and a therapeutical approach to culminate the muscular damage have not been extensively studied. Thus, this study evaluates the impact of rotenone-induced SNPc lesions on skeletal muscle apoptosis and the efficacy of an ethyl acetate extract of Morinda citrifolia in safeguarding the myocytes. Biochemical assays along with apoptotic markers studied by immunoblot and reverse transcription-polymerase chain reaction in the current study revealed that the supplementation of Morinda citrifolia significantly reverted alterations in both biochemical and histological parameters in rotenone-infused PD rats. Treatment with Morinda citrifolia also reduced the expression of pro-apoptotic proteins Bax, caspase-3 and caspase-9 and blocked the release of cytochrome c from mitochondria induced by rotenone. In addition, it augmented the expression of Bcl2 both transcriptionally and translationally. Thus, this preliminary study paves a way to show that the antioxidant and anti-apoptotic activities of Morinda citrifolia can be exploited to alleviate skeletal muscle damage induced by Parkinsonism.

  20. Comparison of Antibacterial Efficacy of Turmeric Extract, Morinda Citrifolia and 3% Sodium Hypochlorite on Enterococcus faecalis: An In-vitro Study

    Science.gov (United States)

    Somisetty, Kusum Valli; Diwan, Abhinav; Pasha, Shiraz; Shetty, Nandaprasad; Reddy, Yashwanth; Nadigar, Shankar

    2016-01-01

    Introduction Sodium hypochlorite (NaOCl), the most commonly used irrigant, has many potential properties like its unique ability to dissolve pulp tissue, excellent antimicrobial activity, but has a cytotoxic effect when injected into periapical tissues. It is also known to produce allergic reactions, foul smell and taste, and potential for corrosion. Facultative organisms such as Enterococcus faecalis and aerobes like Staphylococcus aureus are considered to be the most resistant species and one of the possible causes of root canal treatment failure. So there is a need to find an alternative to sodium hypochlorite to act against these resistant microorganisms. Aim To evaluate and compare the antibacterial efficacy of morinda citrifolia and turmeric extract with 3% NaOCl as a root canal irrigant, against E. faecalis and S.aureus. Materials and Methods The antimicrobial efficacy was assessed in vitro using agar well diffusion method. Agar plates were prepared using Brain-Heart Infusion (BHI) agar. Cultures of E.faecalis and S.aureus were grown in nutrient broth at 37°C. Plates were incubated for 24 hours at 37°C and microbial zones of inhibition were recorded. Statistical analysis was performed using ANOVA. Results NaOCl (3%) showed larger zones of inhibition than herbal irrigants against both the microorganisms. Among the herbal irrigants, morinda citrifolia showed larger zones of inhibition than turmeric hydro-alcoholic extract and turmeric water extract which was statistically significant (pendodontics might prove to be advantageous. PMID:27891459

  1. Comparative evaluation of antimicrobial effect of herbal root canal irrigants (Morinda citrifolia, Azadirachta indica, Aloe vera) with sodium hypochlorite: An in vitro study

    Science.gov (United States)

    Babaji, Prashant; Jagtap, Kiran; Lau, Himani; Bansal, Nandita; Thajuraj, S.; Sondhi, Priti

    2016-01-01

    Aim and Objectives: Successful root canal treatment involves the complete elimination of microorganism from the root canal and the three-dimensional obturation of the canal space. Enterococcus faecalis is the most commonly found bacteria in failed root canal. Chemical irrigation of canals along with biomechanical preparation helps in the elimination of microorganisms. The present study was aimed to evaluate the antimicrobial effect of herbal root canal irrigants (Morinda citrifolia, Azadirachta indica extract, Aloe vera) with sodium hypochlorite (NaOCl). Materials and Methods: The bacterial E. faecalis (ATCC) culture was grown overnight in brain heart infusion (BHI) broth and inoculated in Mueller–Hinton agar plates. Antibacterial inhibition was assessed using agar well diffusion method. All five study irrigants were added to respective wells in agar plates and incubated at 37°C for 24 h. Bacterial inhibition zone around each well was recorded. Results were tabulated and statistically analyzed using Statistical Package for the Social Sciences software for Windows, version 19.0. (IBM Corp., Armonk, NY. Results: Highest inhibitory zone against E. faecalis was seen in NaOCl fallowed by M. citrifolia and A. indica extract, and the least by A. vera extract. Conclusion: Tested herbal medicine (A. indica extract, M. citrifolia, A. vera) showed inhibitory zone against E. faecalis. Hence, these irrigants can be used as root canal irrigating solutions. PMID:27382533

  2. Chemopreventive Effects of Morindia Citrifolia Juice (noni on Experimental Breast Cancer in Rats: Preliminary Study

    Directory of Open Access Journals (Sweden)

    Ana Milena Serrano Contreras

    2014-06-01

    Full Text Available This study determines the effect of Morindia citrifolia juice (Tahitian Noni® in the development of breast cancer induced by carcinogen agent 7.12-dimethylbenzanthracene (DMBA in rats. For this purpose, the breast cancer induction model 1.7-DMBA was used on Spraguey Dawley nulliparous rats of 35 days of age, randomly divided into three groups: group 1 control, which received no treatment, and groups 2 and 3, induced with DMBA at a dose of 55 mg/kg. The latter received a dose of noni juice of 4 ml/kg per day for 90 days. The results showed that a significant percentage (83.33% of the rats from the group induced with DMBA not treated with noni juice developed palpable breast tumors ( ≤ 2 cm of the ductal carcinoma in situ type and atypical ductal hyperplasia, compared to the other groups that did not develop any kind of tumors. In addition, it was found that rats that developed breast cancer had a lower weight gain and significantly increased water consumption (p < 0.05 compared to the other two groups. The results of the hematological and biochemical parameters showed no significant changes between groups. Histopathological changes compatible with liver toxicity were found in rats treated with noni juice. In conclusion, it was found in this preliminary study that noni juice has positive effects in modulating the development of breast cancer induced by DMBA.

  3. Role of aqueous extract of morinda citrifolia (Indian noni ripe fruits in inhibiting dental caries-causing streptococcus mutans and streptococcus mitis.

    Directory of Open Access Journals (Sweden)

    Barani Kumarasamy

    2014-12-01

    Full Text Available Use of alternative medicine to control oral streptococci is a new topic worthy of further investigation. This study aimed to elucidate the dose-dependent anti-bacterial activity of crude aqueous extract of ripe Morinda citrifolia L. (Family: Rubiaceae fruits against oral streptococci i.e. Streptococcus mutans and Streptococcus mitis, that cause dental caries in humans.Fresh ripe M. citrifolia fruits (750g were ground in an electronic blender with sterile water (500ml. The crude aqueous extract was lyophilized to yield a brown colored powder. Various concentrations (1000-100μg/ ml of the extract were tested for its antibacterial activity (Kirby and Bauer method against whole cells of S. mutans and S. mitis. Minimum Inhibitory Concentration (MIC was determined by micro-dilution method, using serially diluted (2 folds fruit extract, according to the National Committee for Clinical Laboratory Standards (NCCLS.Crude aqueous extract (1000μg/ ml of ripe M. citrifolia fruits effectively inhibited the growth of S. mutans (19±0.5 mm and S. mitis (18.6±0.3 mm compared to the streptomycin control (21.6±0.3 mm. The growth inhibition was clearly evident with "nil" bacteriostasis, even after 48 hours of incubation at 37°C. The MIC of the extract for S. mutans and S. mitis was 125 μg and 62.5 μg, respectively.Our results suggest that phytochemicals naturally synthesized by M. citrifolia have an inhibitory effect on oral streptococci. Furthermore, purification and molecular characterization of the "bioactive principle" would enable us to formulate a sustainable oral hygiene product.

  4. HIGH HYDROSTATIC PRESSURE EXTRACTION OF ANTIOXIDANTS FROM MORINDA CITRIFOLIA FRUIT – PROCESS PARAMETERS OPTIMIZATION

    Directory of Open Access Journals (Sweden)

    PRAVEEN KUMAR

    2006-06-01

    Full Text Available A modified version of high hydrostatic pressure extraction has been performed for extraction of antioxidants from M. citrifolia fruit at 5, 15, 25 bar and temperature 30° to 70°C for time duration 1, 2, 4 and 6 hours. The antioxidant activity of the extracts was determined by di-phenylpicrylhydrazyl radical scavenging method. The process parameters were optimized for antioxidant activity by central composite design method of response surface methodology using the statistical package, design expert. The results are expressed as 3D surface graphs. The optimum antioxidant activity was achieved at 58°C and 5 hours for 25bar. The optimal result achieved was within the region of response surface methodology. The statistical results were compared with the experimental result at 25bar, 2hour and 30° to 70°C and were found to be in proximate. The antioxidant activities of the extracts were found to increase with increase in pressure. It was also found that the response surface methodology works effectively for shorter range of parameters considered.

  5. Regeneration of Stem Segments of Noni (Morinda citrifolia Linn.)%诺丽茎段的离体再生

    Institute of Scientific and Technical Information of China (English)

    潘晓晴; 吴田; 蓝增全; 吴腾超; 陶燕蓝

    2014-01-01

    We established two regeneration systems ( Pattern Ⅰ:adventitious roots were induced after adventitious bud;PatternⅡ:adventitious roots were induced before adventitious bud ) with stem segments as explant in MS culture medium with dif-ferent hormones combinations. The best medium for the inducement of callus was 3%MS+1.0 mg/L 6-BA+0.05 mg/L NAA.In the Pattern Ⅰ, the optimal culture medium for the inducement of adventitious bud from the callus was 3%MS+3.0 mg/L 6-BA, and 3%MS+0.2 mg/L NAA was best for the inducement of adventitious roots .In the PatternⅡ, the op-timal culture medium was 3%MS+0.05 mg/L NAA.%以诺丽( Morinda citrifolia Linn.)不含腋芽的茎段薄片为外植体,在添加不同种类和质量浓度的3%MS培养基上离体培养,建立模式Ⅰ为先诱导不定芽后诱导不定根、模式Ⅱ为先诱导不定根后诱导不定芽两种离体再生模式。结果表明:诱导诺丽茎薄片产生愈伤组织的最优培养基为3%MS+1.0 mg/L 6-BA+0.05 mg/L NAA;在模式Ⅰ中,诱导诺丽茎薄片愈伤组织分化出不定芽的最优培养基为3%MS+3.0 mg/L 6-BA,诱导不定芽生根的最优培养基为3%MS+0.2 mg/L NAA;在模式Ⅱ中,诱导诺丽茎薄片的愈伤组织先分化出不定根再分化出不定芽的最优培养基为3%MS+0.05 mg/L NAA。

  6. Effects of Morinda citrifolia aqueous fruit extract and its biomarker scopoletin on reflux esophagitis and gastric ulcer in rats.

    Science.gov (United States)

    Mahattanadul, Sirima; Ridtitid, Wibool; Nima, Sawpheeyah; Phdoongsombut, Narubodee; Ratanasuwon, Pranee; Kasiwong, Srirat

    2011-03-24

    The present study was carried out to evaluate the effect of dried mature unripe Morinda citrifolia L. (Rubiaceae) fruit, commonly known as "Noni", in an aqueous extract preparation (AFE) as used in Thai traditional medicine and its biomarker scopoletin on gastro-esophageal inflammatory models that are related to the claimed pharmacological properties of AFE and/or resembled the human esophagitis or gastric ulcer. The powder of dried mature unripe Noni fruit was boiled in water until it became a sticky paste and was then dried into a powder by lyophilization. The pharmacological activity of AFE and pure scopoletin at the same equivalent dose present in AFE was investigated in rat on gastro-esophageal inflammatory models (acid reflux esophagitis, acute gastritis induced by ethanol and serotonin, and chronic gastric ulcer induced by acetic acid); gastric biochemical parameters and gastrointestinal motility. AFE (0.63-2.50 g/kg) significantly prevented the formation of acid reflux esophagitis, reduced the formation of ethanol-induced acute gastric lesions, suppressed the development of gastric lesions in response to serotonin, and accelerated the healing of acetic acid-induced chronic gastric ulcer in rats with equal potency to those obtained by standard antisecretory agents (ranitidine and lansoprazole). AFE also significantly inhibited gastric acid secretion and pepsin activity in pylorus ligated rats. Additionally, AFE strongly increased the gastrointestinal transit of charcoal meal with a higher potency than cisapride. Pure scopoletin, when compared at the same equivalent dose containing in AFE, possessed similar antiulcer and antisecretory properties to that of AFE although it exerted a less prokinetic activity than AFE. The findings indicated that AFE as well as its biomarker: scopoletin may be beneficial as a potential preventive and therapeutic agent for gastro-esophageal inflammatory diseases, mainly through its antisecretory and prokinetic activities

  7. 169 海巴戟(Morinda)

    Institute of Scientific and Technical Information of China (English)

    梅欣

    2007-01-01

    @@ 又名 Indian Mulberry, Noni, Hog Apple, Menkoedoe,Ruibarbo Caribe, Wild Pine, Tahitian Noni Juice, Bois Douleur, Pau-Azeitona, Mora De La India, Mengkudu,Nhau, Nonu, Nono. 植物名 Morinda citrifolia. 活性成分与药理作用海巴戟的药用部位是其果实、叶和根.海巴戟果实含精油、己酸和辛酸、石蜡以及乙醇酯和甲醇酯.成熟的果实含正己酸.

  8. 海南海巴戟根结线虫病及综合防治%IPM of Morinda citrifolia in Hainan province

    Institute of Scientific and Technical Information of China (English)

    符美英; 陈绵才; 肖彤斌; 王会芳

    2009-01-01

    海巴戟(Morinda citrifolia Linn.)又称诺丽(Noni),是茜草科的常绿小乔木或灌木。主要生长在夏威夷、南太平洋岛屿和东南亚等热带地区。我国海南岛、西沙群岛和台湾岛也有分布,是海南4大南药之一。其抗逆性极强,耐干旱、潮湿,可生长于贫瘠的酸性或碱性土壤上。但是,近年来陆续出现树势衰弱。产量和品质下降问题。

  9. The Role of Endophytic Fungi in the Anticancer Activity of Morinda citrifolia Linn. (Noni)

    OpenAIRE

    Yougen Wu; Sisay Girmay; Vitor Martins da Silva; Brian Perry; Xinwen Hu; Tan, Ghee T.

    2015-01-01

    We hypothesize that the fungal endophytes of noni may possibly play a role in its overall pharmacological repertoire, especially since the perceived efficacy of the fruit in ethnomedicinal use is associated with the fermented juice. The foremost goal of this study is to explore the role of endophyte-derived secondary metabolites in the purported anticancer properties of noni. To that end, culturable endophytic fungi resident within the healthy leaves and fruit of the plant were isolated and i...

  10. Protective effect of Morinda citrifolia fruits on beta-amyloid (25-35) induced cognitive dysfunction in mice: an experimental and biochemical study.

    Science.gov (United States)

    Muralidharan, P; Kumar, V Ravi; Balamurugan, G

    2010-02-01

    The neuroprotective effect of an ethyl acetate extract of Morinda citrifolia (Rubiaceae) Linn. fruits (EMC, ethyl acetate extract of Morinda citrifolia) at doses of 200 and 400 mg/kg, p.o. was studied on beta-amyloid (25-35) peptide induced cognitive dysfunction in mice. In the step-down inhibitory avoidance, EMC exhibited a significant increase in short-term memory and long-term memory (p < 0.05). A significant decrease (p < 0.01) in escape latency was noticed in the animals in the water maze. A significant increase (p < 0.01) in alteration of behavior was exhibited upon administration of EMC 200 and 400 mg/kg on the Y maze. Exploratory parameters such as line crossings, head dipping and rearing were increased significantly in EMC treated groups in a dose-dependent manner (p < 0.05 and p < 0.01). A significant reduction (p < 0.05) in acetyl cholinesterase activity was noticed in the EMC 200 and 400 mg/kg treated groups. The level of monoamine oxidase-A was decreased by the administration of EMC 200 and 400 mg/kg (p < 0.05 and p < 0.01, respectively). EMC at a dose of 400 mg/kg exhibited a significant increase (p < 0.01) in the levels of serotonin and dopamine. Antioxidant enzymes such as superoxide dismutase, glutathione reductase, glutathione peroxidase and ascorbic acid were decreased significantly in the b-amyloid peptide injected group, whose levels were restored significantly (p < 0.01) by the administration of EMC (400 mg/kg).

  11. Comparison of antimicrobial efficacy of propolis, Morinda citrifolia, Azadirachta indica (Neem) and 5% sodium hypochlorite on Candida albicans biofilm formed on tooth substrate: An in-vitro study

    Science.gov (United States)

    Tyagi, Shashi Prabha; Sinha, Dakshita Joy; Garg, Paridhi; Singh, Udai Pratap; Mishra, Chandrakar Chaman; Nagpal, Rajni

    2013-01-01

    Introduction: Endodontic infections are polymicrobial in nature. Candida albicans is the most common fungus isolated from failed endodontic cases. The constant increase in antibiotic resistant strains and side-effects caused by synthetic drugs has prompted researchers to look for herbal alternatives such as propolis, Morinda citrifolia and Azadirachta indica (Neem) etc., since, the gold standard for irrigation, i.e., sodium hypochlorite has many disadvantages. Materials and Methods: Extracted human mandibular premolars were biomechanically prepared, vertically sectioned, placed in tissue culture wells exposing the root canal surface to C. albicans grown on Sabouraud Dextrose Agar to form a biofilm. At the end of 2 days, all groups were treated with test solutions and control for 10 min and evaluated for Candida growth and number of colony forming units. The readings were subjected to statistical analysis using analysis of variance and post hoc Tukey tests. Results: Sodium hypochlorite and propolis groups exhibited highest antimicrobial efficacy against C. albicans with no statistically significant difference. It was followed by the A. indica (Neem) group. M. citrifolia had limited antifungal action followed by the negative control group of saline. Conclusion: According to the results of this study, propolis can be used as an effective antifungal agent similar to that of sodium hypochlorite, although long-term in vivo studies are warranted. PMID:24347888

  12. Comparison of antimicrobial efficacy of propolis, Morinda citrifolia, Azadirachta indica (Neem and 5% sodium hypochlorite on Candida albicans biofilm formed on tooth substrate: An in-vitro study

    Directory of Open Access Journals (Sweden)

    Shashi Prabha Tyagi

    2013-01-01

    Full Text Available Introduction: Endodontic infections are polymicrobial in nature. Candida albicans is the most common fungus isolated from failed endodontic cases. The constant increase in antibiotic resistant strains and side-effects caused by synthetic drugs has prompted researchers to look for herbal alternatives such as propolis, Morinda citrifolia and Azadirachta indica (Neem etc., since, the gold standard for irrigation, i.e., sodium hypochlorite has many disadvantages. Materials and Methods: Extracted human mandibular premolars were biomechanically prepared, vertically sectioned, placed in tissue culture wells exposing the root canal surface to C. albicans grown on Sabouraud Dextrose Agar to form a biofilm. At the end of 2 days, all groups were treated with test solutions and control for 10 min and evaluated for Candida growth and number of colony forming units. The readings were subjected to statistical analysis using analysis of variance and post hoc Tukey tests. Results: Sodium hypochlorite and propolis groups exhibited highest antimicrobial efficacy against C. albicans with no statistically significant difference. It was followed by the A. indica (Neem group. M. citrifolia had limited antifungal action followed by the negative control group of saline. Conclusion: According to the results of this study, propolis can be used as an effective antifungal agent similar to that of sodium hypochlorite, although long-term in vivo studies are warranted.

  13. The Role of Endophytic Fungi in the Anticancer Activity of Morinda citrifolia Linn. (Noni)

    Science.gov (United States)

    Wu, Yougen; Girmay, Sisay; da Silva, Vitor Martins; Perry, Brian; Hu, Xinwen; Tan, Ghee T.

    2015-01-01

    We hypothesize that the fungal endophytes of noni may possibly play a role in its overall pharmacological repertoire, especially since the perceived efficacy of the fruit in ethnomedicinal use is associated with the fermented juice. The foremost goal of this study is to explore the role of endophyte-derived secondary metabolites in the purported anticancer properties of noni. To that end, culturable endophytic fungi resident within the healthy leaves and fruit of the plant were isolated and identified by molecular sequence analysis of the 5.8S gene and internal transcribed spacers (ITS). Purified organisms were subjected to in vitro fermentation in malt extract broth for 8 weeks under anaerobic conditions at room temperature (25°C), in order to simulate the conditions under which traditional fermented noni juice is prepared. The cytotoxic potential of organic extracts derived from the fermented broths of individual endophytes was then tested against three major cancers that afflict humans. Twelve distinct endophytic fungal species were obtained from the leaves and 3 from the fruit. Three of the leaf endophytes inhibited the growth of human carcinoma cell lines LU-1 (lung), PC-3 (prostate), and MCF-7 (breast) with IC50 values of ≤10 μg/mL. PMID:26783408

  14. The Role of Endophytic Fungi in the Anticancer Activity of Morinda citrifolia Linn. (Noni

    Directory of Open Access Journals (Sweden)

    Yougen Wu

    2015-01-01

    Full Text Available We hypothesize that the fungal endophytes of noni may possibly play a role in its overall pharmacological repertoire, especially since the perceived efficacy of the fruit in ethnomedicinal use is associated with the fermented juice. The foremost goal of this study is to explore the role of endophyte-derived secondary metabolites in the purported anticancer properties of noni. To that end, culturable endophytic fungi resident within the healthy leaves and fruit of the plant were isolated and identified by molecular sequence analysis of the 5.8S gene and internal transcribed spacers (ITS. Purified organisms were subjected to in vitro fermentation in malt extract broth for 8 weeks under anaerobic conditions at room temperature (25°C, in order to simulate the conditions under which traditional fermented noni juice is prepared. The cytotoxic potential of organic extracts derived from the fermented broths of individual endophytes was then tested against three major cancers that afflict humans. Twelve distinct endophytic fungal species were obtained from the leaves and 3 from the fruit. Three of the leaf endophytes inhibited the growth of human carcinoma cell lines LU-1 (lung, PC-3 (prostate, and MCF-7 (breast with IC50 values of ≤10 μg/mL.

  15. The Role of Endophytic Fungi in the Anticancer Activity of Morinda citrifolia Linn. (Noni).

    Science.gov (United States)

    Wu, Yougen; Girmay, Sisay; da Silva, Vitor Martins; Perry, Brian; Hu, Xinwen; Tan, Ghee T

    2015-01-01

    We hypothesize that the fungal endophytes of noni may possibly play a role in its overall pharmacological repertoire, especially since the perceived efficacy of the fruit in ethnomedicinal use is associated with the fermented juice. The foremost goal of this study is to explore the role of endophyte-derived secondary metabolites in the purported anticancer properties of noni. To that end, culturable endophytic fungi resident within the healthy leaves and fruit of the plant were isolated and identified by molecular sequence analysis of the 5.8S gene and internal transcribed spacers (ITS). Purified organisms were subjected to in vitro fermentation in malt extract broth for 8 weeks under anaerobic conditions at room temperature (25°C), in order to simulate the conditions under which traditional fermented noni juice is prepared. The cytotoxic potential of organic extracts derived from the fermented broths of individual endophytes was then tested against three major cancers that afflict humans. Twelve distinct endophytic fungal species were obtained from the leaves and 3 from the fruit. Three of the leaf endophytes inhibited the growth of human carcinoma cell lines LU-1 (lung), PC-3 (prostate), and MCF-7 (breast) with IC50 values of ≤10 μg/mL.

  16. Metastasized lung cancer suppression by Morinda citrifolia (Noni) leaf compared to Erlotinib via anti-inflammatory, endogenous antioxidant responses and apoptotic gene activation.

    Science.gov (United States)

    Lim, Swee-Ling; Mustapha, Noordin M; Goh, Yong-Meng; Bakar, Nurul Ain Abu; Mohamed, Suhaila

    2016-05-01

    Metastasized lung and liver cancers cause over 2 million deaths annually, and are amongst the top killer cancers worldwide. Morinda citrifolia (Noni) leaves are traditionally consumed as vegetables in the tropics. The macro and micro effects of M. citrifolia (Noni) leaves on metastasized lung cancer development in vitro and in vivo were compared with the FDA-approved anti-cancer drug Erlotinib. The extract inhibited the proliferation and induced apoptosis in A549 cells (IC50 = 23.47 μg/mL) and mouse Lewis (LL2) lung carcinoma cells (IC50 = 5.50 μg/mL) in vitro, arrested cancer cell cycle at G0/G1 phases and significantly increased caspase-3/-8 without changing caspase-9 levels. The extract showed no toxicity on normal MRC5 lung cells. Non-small-cell lung cancer (NSCLC) A549-induced BALB/c mice were fed with 150 and 300 mg/kg M. citrifolia leaf extract and compared with Erlotinib (50 mg/kg body weight) for 21 days. It significantly increased the pro-apoptotic TRP53 genes, downregulated the pro-tumourigenesis genes (BIRC5, JAK2/STAT3/STAT5A) in the mice tumours, significantly increased the anti-inflammatory IL4, IL10 and NR3C1 expression in the metastasized lung and hepatic cancer tissues and enhanced the NFE2L2-dependent antioxidant responses against oxidative injuries. The extract elevated serum neutrophils and reduced the red blood cells, haemoglobin, corpuscular volume and cell haemoglobin concentration in the lung cancer-induced mammal. It suppressed inflammation and oedema, and upregulated the endogenous antioxidant responses and apoptotic genes to suppress the cancer. The 300 mg/kg extract was more effective than the 50 mg/kg Erlotinib for most of the parameters measured.

  17. Noni juice is not hepatotoxic

    Institute of Scientific and Technical Information of China (English)

    Brett J West; C Jarakae Jensen; Johannes Westendorf

    2006-01-01

    Noni juice (Morinda citrifolia) has been approved for use as a safe food within the European Union, following a review of safety. Since approval, three cases of acute hepatitis in Austrian noni juice consumers have been published, where a causal link is suggested between the liver dysfunction and ingestion of anthraquinones from the plant. Measurements of liver function in a human clinical safety study of TAHITIAN NONI(R) Juice,as well as subacute and subchronic animal toxicity tests revealed no evidence of adverse liver effects at doses many times higher than those reported in the case studies. Additionally, M. Citrifolia anthraquinones occur in the fruit in quantities too small to be of any toxicological significance. Further, these do not have chemical structures capable of being reduced to reactive anthrone radicals, which were implicated in previous cases of herbal hepototoxicity. The available data reveals no evidence of liver toxicity.

  18. 基于ISSR技术研究诺丽种质资源的亲缘关系%Genetic Relationship ofMorinda citrifolia Germplasms by ISSR

    Institute of Scientific and Technical Information of China (English)

    吴田; 蓝增全

    2014-01-01

    In order to understand the genetic relationship ofMorinda citrifolia(noni), the DNA fingerprints of 13 noni germplasm resources of were studied by ISSR markers. The results showed that ten ISSR primers could amplify 183 bands, of which 159 bands were polymorphic, accounting for 86.9%. The genetic similarity coefifcients of 13 noni germplasm resources ranged from 0.464 to 0.784. Cluster analysis showed that 13 noni germplasm resources could be divided into two clads, in which Small fruit as a single group had distant relation with other germplasm resources. Although all noni germplasm resources could not completely separated according external morphological characteristics, the most with the same characteristics could be clustered into the same group or sub-group.%为探讨我国诺丽(Morinda citrifolia)种质资源的亲缘关系,采用ISSR技术对诺丽种质资源的遗传关系进行分析。结果表明,10条ISSR引物对13份诺丽种质资源共扩增出183条带,其中多态性条带有159条,占86.9%。13份诺丽种质的遗传相似系数为0.464~0.784。聚类分析将13份诺丽种质资源聚为两类,其中诺丽小黑种单独聚为一类,与其他12份诺丽种质资源的亲缘关系较远。虽然按照外部形态特征不能将所有诺丽种质完全区分,但具有相同特征的多数种质还是聚在同一类或亚类中。

  19. Study on Free Radical Scavenging in vitro and Antioxidative Activity of Extracts from Cook Noni (Morinda citrifolia ) Juice%库克诺你果汁提取物体外清除自由基及抗氧化活性研究

    Institute of Scientific and Technical Information of China (English)

    李昌; 谢明勇; 聂少平; 陈军辉; Barbara Zimmermann

    2006-01-01

    This study was conducted to investigate the antioxidant and radical scavenging activity of different extracts from Cook noni juice, including noni polysaccharide (noni-ppt), ethanol solute and ethyl acetate extract. Superoxide anion radicals and hydroxyl radicals were generated from autoxidation of pyrogallol (PR) and Fenton reaction (Fe2 +/H2O2). Furthermore 2, 2-Diphenyl-1-picrylhydrazyl (DPPH) method was applied to evaluate the scavenging activity of the extracts. Malondialdehyde (MDA) ,the product of lipid peroxidation (LPO) was induced by adding Fe2+ into yolk homogenate. The ability of preventing LPO was measured by thibarbituric acid (TBA) method. All measurements were performed by spectrophotometric methods. The results suggested that the fraction of ethanol solute and ethyl acetate extract showed significant scavenging and antioxidant ability; L-ascorbic acid served as the standard. Noni-ppt had weaker activity on hydroxyl radical, DPPH radical, and LPO, and could accelerate the procedure of PR autoxidation. The abilities of the extracts may be related to their polarity.%本文对诺你果汁多糖、乙醇溶出物和乙酸乙酯萃取物体外对超氧阴离子(O2-·)、羟自由基(·OH)、DPPH和脂质过氧化(LPO)的抑制作用进行了研究.超氧阴离子(O2-·)由邻苯三酚自氧化产生;羟自由基(·OH)由Fenton反应产生;利用Fe2+诱发卵黄脂蛋白产生丙二醛(NDA),TBA法测定.所有测定均为分光光度法.结果表明,与已知抗氧化剂L-抗坏血酸相比,乙醇溶出物和乙酸乙酯萃取物均有明显的捕捉自由基和抗氧化能力,而多糖捕捉自由基和抗氧化能力很低,且对O2-·没有抑制作用,反而会增加其生成速度.

  20. Effect of noni (Morinda citrifolia Linn.) fruit and its bioactive principles scopoletin and rutin on rat vas deferens contractility: an ex vivo study.

    Science.gov (United States)

    Pandy, Vijayapandi; Narasingam, Megala; Kunasegaran, Thubasni; Murugan, Dharmani Devi; Mohamed, Zahurin

    2014-01-01

    This study examined the effect of methanolic extract of Morinda citrifolia Linn. (MMC) and its bioactive principles, scopoletin and rutin, on dopamine- and noradrenaline-evoked contractility in isolated rat vas deferens preparations. MMC (1-40 mg/mL), scopoletin (1-200 μg/mL), and rutin hydrate (0.6-312.6 μg/mL) dose-dependently inhibited the contractility evoked by submaximal concentrations of both dopamine and noradrenaline, respectively. Haloperidol and prazosin, reference dopamine D2, and α 1-adrenoceptors antagonists significantly reversed the dopamine- and noradrenaline-induced contractions, respectively, in a dose-dependent manner. Interestingly, MMC per se at higher doses (60-100 mg/mL) showed dose-dependent contractile response in rat vas deferens which was partially inhibited by high doses of haloperidol but not by prazosin. These results demonstrated the biphasic effects of MMC on dopaminergic system; that is, antidopaminergic effect at lower concentrations (60 mg/mL). However, similar contractile response at high doses of scopoletin (0.5-5 mg/mL) and rutin hydrate (0.5-5 mg/mL) per se was not observed. Therefore, it can be concluded that the bioactive principles of MMC, scopoletin, and rutin might be responsible for the antidopaminergic and antiadrenergic activities of MMC.

  1. Noni (Morinda citrifolia L.) fruit extract attenuates the rewarding effect of heroin in conditioned place preference but not withdrawal in rodents.

    Science.gov (United States)

    Narasingam, Megala; Pandy, Vijayapandi; Mohamed, Zahurin

    2016-05-20

    The present study was designed to investigate the effect of a methanolic extract of Morinda citrifolia Linn. fruit (MMC) on the rewarding effect of heroin in the rat conditioned place preference (CPP) paradigm and naloxone-precipitated withdrawal in mice. In the first experiment, following a baseline preference test (preconditioning score), the rats were subjected to conditioning trials with five counterbalanced escalating doses of heroin versus saline followed by a preference test conducted under drug-free conditions (post-conditioning score) using the CPP test. Meanwhile, in the second experiment, withdrawal jumping was precipitated by naloxone administration after heroin dependence was induced by escalating doses for 6 days (3×/ day). The CPP test results revealed that acute administration of MMC (1, 3, and 5 g/kg body weight (bw), p.o.), 1 h prior to the CPP test on the 12th day significantly reversed the heroin-seeking behavior in a dose-dependent manner, which was similar to the results observed with a reference drug, methadone (3 mg/kg bw, p.o.). On the other hand, MMC (0.5, 1, and 3 g/kg bw, p.o.) did not attenuate the heroin withdrawal jumps precipitated by naloxone. These findings suggest that the mechanism by which MMC inhibits the rewarding effect of heroin is distinct from naloxone-precipitated heroin withdrawal.

  2. Effect of Noni (Morinda citrifolia Linn. Fruit and Its Bioactive Principles Scopoletin and Rutin on Rat Vas Deferens Contractility: An Ex Vivo Study

    Directory of Open Access Journals (Sweden)

    Vijayapandi Pandy

    2014-01-01

    Full Text Available This study examined the effect of methanolic extract of Morinda citrifolia Linn. (MMC and its bioactive principles, scopoletin and rutin, on dopamine- and noradrenaline-evoked contractility in isolated rat vas deferens preparations. MMC (1–40 mg/mL, scopoletin (1–200 μg/mL, and rutin hydrate (0.6–312.6 μg/mL dose-dependently inhibited the contractility evoked by submaximal concentrations of both dopamine and noradrenaline, respectively. Haloperidol and prazosin, reference dopamine D2, and α1-adrenoceptors antagonists significantly reversed the dopamine- and noradrenaline-induced contractions, respectively, in a dose-dependent manner. Interestingly, MMC per se at higher doses (60–100 mg/mL showed dose-dependent contractile response in rat vas deferens which was partially inhibited by high doses of haloperidol but not by prazosin. These results demonstrated the biphasic effects of MMC on dopaminergic system; that is, antidopaminergic effect at lower concentrations (60 mg/mL. However, similar contractile response at high doses of scopoletin (0.5–5 mg/mL and rutin hydrate (0.5–5 mg/mL per se was not observed. Therefore, it can be concluded that the bioactive principles of MMC, scopoletin, and rutin might be responsible for the antidopaminergic and antiadrenergic activities of MMC.

  3. The use of morinda citrifolia as a green corrosion inhibitor for low carbon steel in 3.5% NaCl solution

    Science.gov (United States)

    Kusumastuti, Rahayu; Pramana, Rakhmad Indra; Soedarsono, Johny W.

    2017-03-01

    The effect and mechanism of green corrosion inhibitor of Morinda Citrifolia (Noni) toward low carbon steel material has been researched. The general background is to develop the cheap and eco-friendly corrosion inhibitor based on components taken from tropical plants that grow +in Indonesia. This research aims to determine the effectiveness of the use of the extracts of noni as green corrosion inhibitor of carbon steel material in aggressive environment. The medium applied for this experiment is 3.5% natrium chloride solution. The variation of the concentration and immersion time duration has been applied as the experimental parameters. All the work was done at room temperature. The corrosion rate was measured by electrochemical polarization method with CMS 600-Gamry instruments and weight loss. The adsorption of inhibitor into the metal surface, which induced bonding formation after immersion was observed by using FTIR method. Inhibition mechanism was observed by polarization curves and fitted by the Langmuir adsorption models. The experimental results show that the higher concentration of inhibitor increasing the inhibition effect. The optimum inhibition is obtained at 3 ppm noni fruit extract, after immersion for about 288 hours. The corrosion rates obtained was 1.385 mpy, with the inhibitor efficiency of 76.92%. The monolayer film is formed coating the surface material as a result of mixed type corrosion inhibitor behavior of Noni. It can be concluded that this green inhibitor is effective to be used for low carbon steel material.

  4. An Investigation into the Antiobesity Effects of Morinda citrifolia L. Leaf Extract in High Fat Diet Induced Obese Rats Using a 1H NMR Metabolomics Approach

    Directory of Open Access Journals (Sweden)

    Najla Gooda Sahib Jambocus

    2016-01-01

    Full Text Available The prevalence of obesity is increasing worldwide, with high fat diet (HFD as one of the main contributing factors. Obesity increases the predisposition to other diseases such as diabetes through various metabolic pathways. Limited availability of antiobesity drugs and the popularity of complementary medicine have encouraged research in finding phytochemical strategies to this multifaceted disease. HFD induced obese Sprague-Dawley rats were treated with an extract of Morinda citrifolia L. leaves (MLE 60. After 9 weeks of treatment, positive effects were observed on adiposity, fecal fat content, plasma lipids, and insulin and leptin levels. The inducement of obesity and treatment with MLE 60 on metabolic alterations were then further elucidated using a 1H NMR based metabolomics approach. Discriminating metabolites involved were products of various metabolic pathways, including glucose metabolism and TCA cycle (lactate, 2-oxoglutarate, citrate, succinate, pyruvate, and acetate, amino acid metabolism (alanine, 2-hydroxybutyrate, choline metabolism (betaine, creatinine metabolism (creatinine, and gut microbiome metabolism (hippurate, phenylacetylglycine, dimethylamine, and trigonelline. Treatment with MLE 60 resulted in significant improvement in the metabolic perturbations caused obesity as demonstrated by the proximity of the treated group to the normal group in the OPLS-DA score plot and the change in trajectory movement of the diseased group towards the healthy group upon treatment.

  5. Morinda citrifolia (Noni as an Anti-Inflammatory Treatment in Women with Primary Dysmenorrhoea: A Randomised Double-Blind Placebo-Controlled Trial

    Directory of Open Access Journals (Sweden)

    H. M. Fletcher

    2013-01-01

    Full Text Available Introduction. Noni (Morinda citrifolia has been used for many years as an anti-inflammatory agent. We tested the efficacy of Noni in women with dysmenorrhea. Method. We did a prospective randomized double-blind placebo-controlled trial in 100 university students of 18 years and older over three menstrual cycles. Patients were invited to participate and randomly assigned to receive 400 mg Noni capsules or placebo. They were assessed for baseline demographic variables such as age, parity, and BMI. They were also assessed before and after treatment, for pain, menstrual blood loss, and laboratory variables: ESR, hemoglobin, and packed cell volume. Results. Of the 1027 women screened, 100 eligible women were randomized. Of the women completing the study, 42 women were randomized to Noni and 38 to placebo. There were no significant differences in any of the variables at randomization. There were also no significant differences in mean bleeding score or pain score at randomization. Both bleeding and pain scores gradually improved in both groups as the women were observed over three menstrual cycles; however, the improvement was not significantly different in the Noni group when compared to the controls. Conclusion. Noni did not show a reduction in menstrual pain or bleeding when compared to placebo.

  6. Cell cycle arrest and mechanism of apoptosis induction in H400 oral cancer cells in response to Damnacanthal and Nordamnacanthal isolated from Morinda citrifolia.

    Science.gov (United States)

    Shaghayegh, Gohar; Alabsi, Aied M; Ali-Saeed, Rola; Ali, Abdul Manaf; Vincent-Chong, Vui King; Zain, Rosnah Binti

    2016-10-01

    Oral cancer is the eleventh most prevalent cancer worldwide. The most prevalent oral cancer is oral squamous cell carcinoma (OSCC). Damnacanthal (DAM) and nordamnacanthal (NDAM), the anthraquinone compounds, are isolated from the root of Morinda citrifolia L. (Noni), which has been used for the treatment of several chronic diseases including cancer. The objectives of this study were to evaluate the cytotoxicity, cell death mode, cell cycle, and the molecular mechanism of apoptosis induced by DAM and NDAM on OSCC. The cytotoxic effects of these compounds against OSCC cell lines were determined by MTT assay. The cell death mode was analysed by DNA laddering and FITC-annexin V/PI flow cytometric assays. In addition, the mechanism of apoptosis induced by DAM and NDAM was detected using mitochondrial membrane potential, Cytochrome c, and caspases assays. Finally, the effect of DAM and NDAM on cell cycle phase distribution of OSCC cells was detected by flow cytometry. In the present study, DAM and NDAM showed cytotoxicity towards OSCC cell lines and the maximum growth inhibition for both compounds was observed in H400 cells with IC50 value of 1.9 and 6.8 μg/ml, respectively, after 72 h treatment. The results also demonstrated the inhibition of H400 OSCC cells proliferation, internucleosomal cleavage of DNA, activation of intrinsic apoptosis pathway, and cell cycle arrest caused by DAM and NDAM. Therefore, these findings suggest that DAM and NDAM can be potentially used as antitumor agents for oral cancer therapy.

  7. An Investigation into the Antiobesity Effects of Morinda citrifolia L. Leaf Extract in High Fat Diet Induced Obese Rats Using a (1)H NMR Metabolomics Approach.

    Science.gov (United States)

    Gooda Sahib Jambocus, Najla; Saari, Nazamid; Ismail, Amin; Khatib, Alfi; Mahomoodally, Mohamad Fawzi; Abdul Hamid, Azizah

    2016-01-01

    The prevalence of obesity is increasing worldwide, with high fat diet (HFD) as one of the main contributing factors. Obesity increases the predisposition to other diseases such as diabetes through various metabolic pathways. Limited availability of antiobesity drugs and the popularity of complementary medicine have encouraged research in finding phytochemical strategies to this multifaceted disease. HFD induced obese Sprague-Dawley rats were treated with an extract of Morinda citrifolia L. leaves (MLE 60). After 9 weeks of treatment, positive effects were observed on adiposity, fecal fat content, plasma lipids, and insulin and leptin levels. The inducement of obesity and treatment with MLE 60 on metabolic alterations were then further elucidated using a (1)H NMR based metabolomics approach. Discriminating metabolites involved were products of various metabolic pathways, including glucose metabolism and TCA cycle (lactate, 2-oxoglutarate, citrate, succinate, pyruvate, and acetate), amino acid metabolism (alanine, 2-hydroxybutyrate), choline metabolism (betaine), creatinine metabolism (creatinine), and gut microbiome metabolism (hippurate, phenylacetylglycine, dimethylamine, and trigonelline). Treatment with MLE 60 resulted in significant improvement in the metabolic perturbations caused obesity as demonstrated by the proximity of the treated group to the normal group in the OPLS-DA score plot and the change in trajectory movement of the diseased group towards the healthy group upon treatment.

  8. Morinda citrifolia (Noni) as an Anti-Inflammatory Treatment in Women with Primary Dysmenorrhoea: A Randomised Double-Blind Placebo-Controlled Trial.

    Science.gov (United States)

    Fletcher, H M; Dawkins, J; Rattray, C; Wharfe, G; Reid, M; Gordon-Strachan, G

    2013-01-01

    Introduction. Noni (Morinda citrifolia) has been used for many years as an anti-inflammatory agent. We tested the efficacy of Noni in women with dysmenorrhea. Method. We did a prospective randomized double-blind placebo-controlled trial in 100 university students of 18 years and older over three menstrual cycles. Patients were invited to participate and randomly assigned to receive 400 mg Noni capsules or placebo. They were assessed for baseline demographic variables such as age, parity, and BMI. They were also assessed before and after treatment, for pain, menstrual blood loss, and laboratory variables: ESR, hemoglobin, and packed cell volume. Results. Of the 1027 women screened, 100 eligible women were randomized. Of the women completing the study, 42 women were randomized to Noni and 38 to placebo. There were no significant differences in any of the variables at randomization. There were also no significant differences in mean bleeding score or pain score at randomization. Both bleeding and pain scores gradually improved in both groups as the women were observed over three menstrual cycles; however, the improvement was not significantly different in the Noni group when compared to the controls. Conclusion. Noni did not show a reduction in menstrual pain or bleeding when compared to placebo.

  9. An Investigation into the Antiobesity Effects of Morinda citrifolia L. Leaf Extract in High Fat Diet Induced Obese Rats Using a 1H NMR Metabolomics Approach

    Science.gov (United States)

    Gooda Sahib Jambocus, Najla; Saari, Nazamid; Ismail, Amin; Mahomoodally, Mohamad Fawzi; Abdul Hamid, Azizah

    2016-01-01

    The prevalence of obesity is increasing worldwide, with high fat diet (HFD) as one of the main contributing factors. Obesity increases the predisposition to other diseases such as diabetes through various metabolic pathways. Limited availability of antiobesity drugs and the popularity of complementary medicine have encouraged research in finding phytochemical strategies to this multifaceted disease. HFD induced obese Sprague-Dawley rats were treated with an extract of Morinda citrifolia L. leaves (MLE 60). After 9 weeks of treatment, positive effects were observed on adiposity, fecal fat content, plasma lipids, and insulin and leptin levels. The inducement of obesity and treatment with MLE 60 on metabolic alterations were then further elucidated using a 1H NMR based metabolomics approach. Discriminating metabolites involved were products of various metabolic pathways, including glucose metabolism and TCA cycle (lactate, 2-oxoglutarate, citrate, succinate, pyruvate, and acetate), amino acid metabolism (alanine, 2-hydroxybutyrate), choline metabolism (betaine), creatinine metabolism (creatinine), and gut microbiome metabolism (hippurate, phenylacetylglycine, dimethylamine, and trigonelline). Treatment with MLE 60 resulted in significant improvement in the metabolic perturbations caused obesity as demonstrated by the proximity of the treated group to the normal group in the OPLS-DA score plot and the change in trajectory movement of the diseased group towards the healthy group upon treatment. PMID:26798649

  10. PENGARUH PENGGUNAAN TEPUNG SILASE DAUN MENGKUDU (Morinda citrifolia) DALAM FORMULA PAKAN TERHADAP PERTUMBUHAN IKAN SIDAT (Anguilla bicolor) STADIA ELVER

    OpenAIRE

    Dewi Cholifah; Mivida Febriani; Ekawati, Arning W; Yenny Risjani

    2012-01-01

    Penelitian ini dilakukan untuk mengetahui pengaruh dan dosis terbaik penggunaan tepung silase daun Mengkudu (M. citrifolia) dalam formula pakan terhadap pertumbuhan ikan Sidat (A. bicolor) stadia elver. Penelitian dibagi menjadi dua tahap, tahap pertama formulasi pakan percobaan, dan tahap kedua uji in vivo/uji formula pakan percobaan skala laboratorium pada ikan Sidat. Pelaksanaan penelitian tahap 1 adalah membuat formula pakan iso protein (40% ) dan iso energi (3.600 kkal/kg pakan) dengan m...

  11. 海巴戟DNA提取方法及SRAP分子标记聚类分析%DNA Extraction and SRAP Clustering Analysis of Morinda citrifolia

    Institute of Scientific and Technical Information of China (English)

    赖茂良; 孙仁毅; 程江波; 聂风琴; 邢诒旺; 林道哲; 符文英

    2014-01-01

    应用SRAP分子标记技术开展海巴戟(Morinda citrifolia Linn,又名Noni)种质资源遗传多样性研究.结果表明,采用改良DNA提取方法能获得质量好,纯度高的海巴戟DNA.应用12对引物对78份海巴戟种质的SRAP分子标记扩增,共获得11 154条带,其中多态性条带有3 792条,多态性为33.99%,种质间具有很好的多态性.聚类分析结果表明,遗传距离为0.66时,78份海巴戟种质可归为2类,即海南本地种和西沙群岛种聚为一类,其余种质则聚为另一类;以0.86为阈值,78份海巴戟种质材料可聚为6类,从上至下依次是:万维2号种、新加坡种、大溪地种、万维1号种、海南本地种、西沙群岛种.其中,大溪地种和万维1号种的相似度较高,可以推断这2个种质亲缘关系较近,新加坡种与万维2号种的亲缘关系次之.

  12. Aktivitas Imunomodulator Ekstrak Buah Mengkudu pada Mencit yang Diinfeksi Staphylococcus aureus (IMMUNOMODULATORS ACTIVITY OF NONI (MORINDA CITRIFOLIA L. FRUIT EXTRACT IN MICE INFECTED WITH STAPHYLOCOCCUS AUREUS

    Directory of Open Access Journals (Sweden)

    Zumrotul Mufidah

    2013-12-01

    Full Text Available This study aim was to determine the immunomodulatory activity of noni (Morinda citrifolia L. fruitextract in mice infected with Staphylococcus aureus. Mice were divided into two group :  non-infected  andinfected. Non Infected group was without S. aureus infection whereas the infected group was infected withS. aureus. Group contain control, dose 1 (25 mg/kg BW, dose 2 (100 mg/kg BW, and dose 3 (300 mg/kg BW.Oral treatment carried out for 20 days in every morning and each sample was injected with  S. aureus atday 21 with 109 cell/mL. Relative number of T cell (CD4+, CD4+CD25+, and cytokine interferon-ã fromCD4+ T cell (CD4+IFN-ã+ subsets was measured using the BD FACSCaliburTM Flowcytometer. Data wereanalyzed by using Analysis of Varians (p<0,05 and SPSS 16 for windows. The result showed thatadministration of noni crude extract was significantly change the relative number of CD4+, CD4+IFN-ã+,and CD4+CD25+ T cells. Treatment of noni crude extract in non-infection group could increase  relativenumber of CD4+, CD4+IFN-ã+  and CD4+CD25+ T cells that might be caused by active compounds of noni asmitogen.  Giving of noni crude extract in infected group could reduce  the relative number of CD4+, CD4+CD25+and CD4+IFN-ã+ T cells due to it  active compounds as anti-inflamation. Noni fruit extract can be used aspreventive therapy on S. aureus infection  because it contains active compounds as an anti-inflammationeffect.

  13. Peran Ekstrak Etanol Topikal Daun Mengkudu (Morinda citrifolia L. pada Penyembuhan Luka Ditinjau dari Imunoekspresi CD34 dan Kolagen pada Tikus Galur Wistar

    Directory of Open Access Journals (Sweden)

    Indah Puti Rahmayani S

    2013-12-01

    Full Text Available Problems in wound healing occurred if proper care is not given and the wound develops into a chronic wound. Noni (Morinda citrifolia L. is one of the most common plants in tropical areas, including Indonesia, which fruit, leaves dan root are used in traditional treatment, for example wound healing. This experimental research with post test-only control group design identified the effect of topical application of noni leaves ethanol extract on wound healing by examining the histopathological appearance of fibroblas count, inflammatory cell infiltration, cluster of differentiation 34 (CD34 immunoexpression, and collagen deposition. The research was performed between November 2010 until September 2011 at the Pharmacology and Pathology Anatomy laboratories of Dr. Hasan Sadikin General Hospital Bandung. Excisions were made on each back of the rat of the 36 rats that were divided into control and test groups where the test group received topical application of noni leaves ethanol extract. The wound was examined on day 3, 7, and 14 using a light microscope. The result showed a tendency of better wound healing in the test group for all parameters based on the data on day 3, 7, and 14. Mann-Whitney Test with 95% confidence interval (p<0.05 showed that the p value for fibroblast count, inflammation cell infiltration, CD34 immunoexpression and collagen subsequently were p=0.319, p=0.290, p=0.251, and p=0.245, respectively. In conclusion, topical application of noni leaves ethanol extract has a benefit on wound healing although the results are not statistically significant.

  14. Larvicidal activity of titanium dioxide nanoparticles synthesized using Morinda citrifolia root extract against Anopheles stephensi, Aedes aegypti and Culex quinquefasciatus and its other effect on non-target fish

    Directory of Open Access Journals (Sweden)

    Thodhal Yoganandham Suman

    2015-03-01

    Full Text Available Objective: To assess the larvicidal activity of titanium dioxide nanoparticles (TiO 2NPS synthesized from the root aqueous extract of Morinda citrifolia (M. citrifolia against the larvae of Anopheles stephensi (An. stephensi, Aedes aegypti (Ae. aegypti and Culex quinquefasciatus (Cx. quinquefasciatus. Methods: The M. citrifolia broth solution was prepared by taking 8 g of the powdered root of M. citrifolia in 250 mL Erlenmeyer flask along with 100 mL of distilled water and boiled for 5 min. About 20 mL of M. citrifolia root extract was added into the 80 mL of an aqueous solution of 5 mmol/L TiO(OH2 for the reduction under continuous stirring for 4 h at 50 °C. Synthesized TiO2NPS were characterized by X-ray diffraction, Fourier transform infrared spectroscopy, field emission scanning electron microscopy and energy dispersive X-ray spectroscopy. X-ray diffraction confirmed the crystalline nature of the nanoparticles. Toxicity studies were carried out against non-target fish species Poecilia reticulata, the most common organism in the habitats of An. stephensi, Ae. aegypti and Cx. quinquefasciatus. Results: The Fourier transform infrared spectroscopy for TiO2NPS synthesized by M. citrifolia root extract showed band at 3 426 cm-1, 1 637 cm-1 and 714 cm-1. The 3 426 cm-1 showed O-H stretching due to alcoholic group; 1 637 cm-1 showed N-H bend due to alcoholic group. In particular, the 1 637 cm-1 indicated the presence of H bend bond for 1° for proteins. A peak was observed around 714 cm-1 due to Ti-O-O bond. Field emission scanning electron microscopy and transmission electron microscopy showed the spherical nature of the nanoparticles with a size of 20.46-39.20 nm. The biosynthesized TiO2NPS showed maximum activity against the larvae of An. stephensi, Ae. aegypti and Cx. quinquefasciatus when compared to the aqueous extract of M. citrifolia. Toxicity studies revealed no toxicity towards Poecilia reticulata at LC50 and LC90 doses of TiO 2NPS

  15. Hepatotoxicity of NONI juice: Report of two cases

    Institute of Scientific and Technical Information of China (English)

    Vanessa Stadlbauer; Peter Fickert; Carolin Lackner; Jutta Schmerlaib; Peter Krisper; Michael Trauner; Rudolf E Stauber

    2005-01-01

    NONI juice (Morinda citrifolia) is an increasingly popular wellness drink claimed to be beneficial for many illnesses.No overt toxicity has been reported to date. We present two cases of novel hepatotoxicity of NONI juice. Causality of liver injury by NONI juice was asses-sed. Routine laboratory tests and transjugular or percutaneous liver biopsy were performed. The first patient underwent successful liver transplantation while the second patient recovered spontaneously after cessation of NONI juice.A 29-year-old man with previous toxic hepatitis associated with small doses of paracetamol developed sub-acute hepatic failure following consumption of 1.5 L NONI juice over 3 wk necessitating urgent liver transplantation. A 62-year-old woman without evidence of previous liver disease developed an episode of self-limited acutehepatitis following consumption of 2 L NONI juice for over 3 mo. The most likely hepatotoxic components of Morinda citrifolia were anthraquinones. Physicians should be aware of potential hepatotoxicity of NONI juice.

  16. 诺丽籽的化学成分%Chemical Constituents from the Seeds of Morinda citrifolia

    Institute of Scientific and Technical Information of China (English)

    杨小龙; 蒋孟圆; 谢坤龙; 刘吉开

    2009-01-01

    目的:研究诺丽籽化学成分.方法:利用硅胶柱层析、反相RP-18、半制备薄层层析和凝胶柱层析对诺丽籽进行分离纯化,借助光谱学手段鉴定其结构.结果:分离得到20个已知化合物:胡萝卜甾醇(1),乌苏酸(2),19-羟基-乌苏酸(3),1,5,15-trimethylmorindol(4),5,15-dimethyl-morindol(5),东茛菪亭(6),3,3′-双二甲基松脂素(7),3,4,3′4′-四羟基-9,7′α-过氧化木脂素-7α,9′-丁内酯(8),americanin D(9),americanin A(10),americanin(11),isoprincepin(12),去乙酰车叶草苷酸(13),落干酸(14),车叶草苷酸(15),rhodolatouside(16),芦丁(17),4-乙基-2羟基-丁二酸(18),5-羟甲基-2-呋喃甲醛(19),3-甲基丁基-3-烯基海藻糖(20).结论:化合物9、11、14、16和18首次从该植物中分离得到.%AIM: To investigate the chemical constituents of the seeds of M.Citrifolia.METHODS: The chemical constituents from the seed of M.Citrifolia were separated and purified by silica gel.Rp-18, Sephadex LH-20 column chroma- tography and Pre-TLC.Their structures were elucidated on the basis of extensive spectroscopic analysis (including 2D NMR).RESULTS: Twenty known compounds were identified as daucosterol (1), ursolic acid (2), 19-hydroxyl-ursolic acid (3), 1, 5, 15-trimethylmorindol (4), 5, 15-dimethyl-morindol (5), scopoletin (6), 3, 3'-bisdemethylpinoresinoi (7), 3, 4, 3' 4'- tetrahy- droxy-9, 7'α-epoxylignano-7α, 9'-lactone (8), americanin D (9), americanin A (10), americanin (11), isoprincepin (12), deace- tyl-asperulosidic acid (13), loganic acid (14), asperulosidic acid (15), rhodolatouside (16), quercetin-3-O-α-L- rhamnopyranosyl-(1→6)-β-D-glucopyranoside (17), 4-ethyl-2-hydroxyl-succinate (18), 5-hydroxymethyl-2-furancarbox- aldehyde (19), 3-methylbut-3-enyl-6-O-β-D-glucopyranosyl-β-D-glucopyranoside (20).

  17. Composição centesimal, compostos bioativos e atividade antioxidante de frutos de Morinda citrifolia Linn (noni cultivados no Paraná

    Directory of Open Access Journals (Sweden)

    G.F. PALIOTO

    2015-03-01

    Full Text Available O fruto de noni (Morinda citrifolia L. é consumido há milênios na medicina popular polinésia devido aos benefícios nutricionais e terapêuticos. O consumo de noni em outros países, incluindo o Brasil, cresceu vertiginosamente nos últimos anos em decorrência das atividades biológicas atribuídas a ingestão do suco da fruta, principalmente pela propriedade anticâncer. Contudo, a composição química da planta, que está relacionada com suas propriedades biológicas, é determinada pelo seu local de origem, e por influência do clima e do solo onde é cultivada. Neste sentido, este trabalho teve como objetivo analisar a polpa extraída de frutos maduros de noni cultivados em Maringá-PR. A análise da polpa in natura apresentou 89,16% de umidade, 0,75% de cinzas, 2,10% de proteínas, 2,19% de lipídios e 5,81% de carboidratos. Dos compostos bioativos, foram analisados antocianinas (1,39 mg.100 g-1 polpa, flavonoides amarelos (13,01 mg.100 g-1 polpa, carotenóides (0,45 mg.100 g-1 polpa e vitamina C (12,16 mg.100 g-1 polpa. Para fenólicos totais e atividade antioxidante foram preparados diferentes extratos, sendo que os maiores teores de fenólicos totais foram encontrados no extrato aquoso (1143,56 mg equivalente de ácido gálico (EAG.100 g-1, seguido do extrato etanólico (966,96 mg EAG.100 g-1, metanol/acetona (820,88 mg EAG.100 g-1 e metanólico (306,33 mg EAG.100 g-1. Os melhores resultados para antioxidantes, determinado pelo EC50 - concentração do extrato necessária para reduzir 50% do radical DPPH, foram encontrados nos extratos metanol/acetona (EC50 de 25,18 mg.mL-1 e metanólico (EC50 de 25,96 mg.mL-1. A atividade antioxidante dos frutos pode estar relacionada com o conteúdo de vitamina C, uma vez que os extratos com um menor conteúdo de fenóis totais foram aqueles que apresentaram menores valores de EC50.

  18. Chemical constituents from fruits of Morinda citrifolia%诺丽果的化学成分研究

    Institute of Scientific and Technical Information of China (English)

    刘树民; 王宇; 张洪财; 苗静囡; 邱军强; 王可心

    2012-01-01

    目的 对诺丽Morinda citrifolia新鲜果实进行化学成分研究,为其开发利用提供依据.方法 应用硅胶柱色谱、ODS、制备型高效液相色谱等方法进行分离纯化,通过波谱解析进行结构鉴定.结果 从新鲜诺丽果正丁醇萃取组分中分离得到11个化合物,分别鉴定为3-甲基丁-3-烯基-6-O-β-D-吡喃葡萄糖基-β-D-吡喃葡糖苷(1)、xeroboside (2)、3-甲氧基-4-羟基-5-[(8'S)-3-甲氧基-4-羟苯丙基醇]-E-肉桂醇-4-O-β-D-吡喃葡萄糖苷(3)、canthoside A(4)、(2R,3S)-2,3-dihydro-2-(4'-hydroxy-3'-methoxy-phenyl)-3-(hydroxymethyl)-7-methoxy-5-benzofuranpropapo1-4'-O-β-glucopyranoside (5)、异落叶松脂素-2a-O-β-D-吡喃葡萄糖苷(6)、淫羊藿次苷D2 (7)、松柏苷(8)、2-甲氧基-4-(2'-羟乙基)-苯酚-1-O-β-D-吡喃葡萄糖苷(9)、七叶苷(10)、3-甲基丁-3-烯基-β-D-吡喃葡萄糖苷(11).结论 化合物2~4为首次从该属植物中分离得到,化合物5~10为首次从该植物中分离得到.

  19. 诺丽叶片DNA的提取及ISSR-PCR 反应体系的建立%Isolation of Genomic DNA in Morinda citrifolia Linn. and Establishment of Its ISSR-PCR Reaction System

    Institute of Scientific and Technical Information of China (English)

    吴田; 蓝增全; 李青红

    2010-01-01

    [目的]提取诺丽(Morinda citrifolia Linn.)叶片DNA,并建立ISSR-PCR反应体系.[方法]以3份采自海南、4份采自美国的诺丽种质的叶片为材料,对其DNA提取和ISSR分子标记方法进行了研究.[结果]采用改进的CTAB DNA微量提取法,可以得到高质量的诺丽叶片基因组DNA.用14条不同的ISSR引物对所提取的诺丽基因组DNA进行了ISSR分子标记分析,其中7条引物在诺丽DNA中可扩增出多态性产物.[结论]建立了诺丽叶片基因组DNA快速、高效的提取方法和ISSR标记体系,可为ISSR分析应用于诺丽遗传研究奠定良好的基础.

  20. Progress in research on the physiological function of Morinda citrifolia L.%海巴戟果实诺丽(NONI)生理功能研究进展

    Institute of Scientific and Technical Information of China (English)

    于纯淼; 李煦照; 于栋华; 陈佳; 刘树民

    2011-01-01

    Morinda citrifolia L. (Noni)had been widely used as folk drugs by Polynesian,which had more than 2000 years. In recent years,the scholars at home and abroad had done a more in-depth research on its physiological function. The research of advancement in noni were reviewed,such as protection of liver damage,immune regulation,anti-tumor and anti-oxidation. And put forward the prospect of its application.%海巴戟(诺丽)被波利尼西亚人作为民间药物而广泛使用已有2000多年历史。近几年,国内外学者对其生理功能做了较为深入的研究。现就其对肝损伤的保护、免疫调节、抗肿瘤、抗氧化等方面的研究进展做一综述,并对应用前景进行了展望。

  1. Uji Aktivitas Beberapa Konsentrasi Ekstrak Daun Dan Buah Mengkudu (Morinda citrifolia L.) Terhadap Ulat Tritip Plutella Xylostella L. (Lepidoptera : Plutellidae) Di Laboratorium

    OpenAIRE

    Ginting, Maria

    2012-01-01

    This research is aim to to know activity from leaf extract and mengkudu fruit (M L citrifolia.) to tritip caterpillar ( P. xylostella L.). This Research is executed on Laboratory Pest Plant Faculty Of Agriculture University North Sumatra with height + 25 metre highland and was done on April - May 2005. This research use Factorial Complete Random Device with 2 Factor that is DB (Extract Leaf and Mengkudu fruit) and K ( Concentration). Consist with 6 treatment and 4 replicates. That is DK...

  2. Uji Aktivitas Beberapa Konsentasi Ekstrak Daun dan Buah Mengkudu (Morinda citrifolia L.) Terhadap Ulat Tritip Plutella xyU>stella L. (Lepidoptera : Plutellidae) Di Laboratorium

    OpenAIRE

    Ginting, Maria

    2012-01-01

    This research is aim to to know activity from leaf extract and mengkudu fruit (M L citrifolia.) to tritip caterpillar ( P. xylostella L.). This Research is executed on Laboratory Pest Plant Faculty Of Agriculture University North Sumatra with height + 25 metre highland and was done on April - May 2005. This research use Factorial Complete Random Device with 2 Factor that is DB (Extract Leaf and Mengkudu fruit) and K ( Concentration). Consist with 6 treatment and 4 replicates. That is...

  3. Antioxidant activity of noni juice in heavy smokers

    Directory of Open Access Journals (Sweden)

    Wang Mian-Ying

    2009-10-01

    Full Text Available Abstract Background Noni (Morinda citrifolia juice has demonstrated antioxidant activity in vitro and in vivo. To evaluate this activity in humans, noni juice from Tahiti (TNJ was evaluated in a 30 day, double-blind, and placebo controlled clinical trial with 285 current heavy smokers. Research participants were randomly assigned to three daily treatment groups: 118 mL placebo, 29.5 mL TNJ, and 118 mL TNJ. Plasma superoxide anion radicals (SAR and lipid hydroperoxide (LOOH levels were measured pre and post-intervention. Results After 30 days, mean SAR decreased from 0.26 ± 0.14 to 0.19 ± 0.10 μmol/mL in the 29.5 mL dose group (P P P P Conclusion The results suggest an antioxidant activity from noni juice in humans exposed to tobacco smoke, thereby replicating the results found previous chemical and in vivo tests.

  4. Effectivity of water soluble granule from kenikir leaves extract (Cosmos caudatus, noni leaves extract (Morinda citrifolia, and earthworm meal extract (Lumbricus rubellus as a natural coccidiostat for broiler chickens against infection caused by Eimeri

    Directory of Open Access Journals (Sweden)

    Karimy MF

    2013-06-01

    Full Text Available The aim of this research was to study effectivity of water soluble granule from kenikir leaves extract (Cosmos caudatus, noni leaves extract (Morinda citrifolia, and earthworm meal extract (Lumbricus rubellus as a natural coccidiostat for broiler chickens against infection caused by Eimeria tenella. One hundred day old chick (DOC of the Cobb strain broiler were randomly devided into 10 groups and each group consisted of 10 chickens. All groups were orally infected by 5000 sporulated oocyst of E. tenella on the 25th days old as a challenge infection. The chickens was treated by granule of kenikir leaves extract, noni leaves extract and granule of earthworm meal extract which level dosage was 100, 200 and 300 mg/kgbw, respectively on each treatment (K1, K2, K3; M1, M2, M3 and T1, T2, T3. Control (K0 did not treated by feed additive. Treatment was administered on drinking water. On the 5th days after challenge infection 5 chickens of each groups were slaughtered and necropted to evaluate lession score and histopatology of caeca. Oocyst per gram excreta was count on 7th days until 10th days after challenge infection of the others 5 chickens of each groups. The results showed that the lowest score of lession was obtained on M2 and M3 whereas the lowest total oocyst per gram excreta was obtained on M3. Histopathological observation revealed that there was no stadia development of E. tenella in M2 treatment. It was concluded that granule of noni leaves extract at 200 mg/kgbw (M2 was the most effective natural coccidiostat.

  5. Influência da matéria orgânica e do ambiente no crescimento e acúmulo de solutos em Morinda citrifolia submetida à salinidade

    Directory of Open Access Journals (Sweden)

    Maria Cristina Martins Ribeiro de Souza

    2014-09-01

    Full Text Available O Noni (Morinda citrifolia L. é uma planta tolerante aos efeitos salinos e alcalinos dos solos e desenvolve-se em regiões de clima seco ou úmido. Apesar da adaptabilidade intercontinental, ainda são poucas as informações sobre sua tolerância aos sais no solo brasileiro. Baseado no exposto, objetivou-se com o presente trabalho avaliar os efeitos da água salina no crescimento inicial do Noni. Para isso, foi conduzido um experimento em vasos de polietileno com capacidade para 20 L, em Sobral, Ceará. O delineamento estatístico adotado foi em blocos ao acaso, disposto no esquema de parcelas subsubdivididas, com 5 repetições. As parcelas foram constituídas pelos ambientes de cultivo (céu aberto e telado, as subparcelas pelos níveis de salinidade da água de irrigação (CEa: 0,3;1,5; 3,0; 4,5 e 6,0 dS m-1 e as subsubparcelas foram representadas pela ausência e presença de matéria orgânica. Aos 110 dias após a aplicação dos tratamentos (DAT, avaliaram-se: altura, massa seca da parte aérea (MSPA, da raiz (MSR, total (MST e área foliar (AF, e aos 90 (DAT os teores foliares de solutos orgânicos. Todas as variáveis de crescimento estudadas foram afetadas negativamente pela salinidade, sendo que o efeito observado a céu aberto foi mais expressivo do que no ambiente telado. A matéria orgânica não teve efeito atenuante sobre os efeitos deletérios da salinidade em plantas de noni. O acúmulo de prolina mostrou-se um sinalizador de dano causado pelo estresse, visto que os maiores acúmulos ocorreram nas plantas que sofreram maiores reduções no crescimento.

  6. 诺尼抗肿瘤作用及其机制的研究进展%Progress on antitumor activity and its mechanisms of Morinda citrifolia

    Institute of Scientific and Technical Information of China (English)

    陈建国; 李金霞(综述); 程池(审校)

    2014-01-01

    本文概述了近十多年来国内外有关诺尼抗肿瘤作用及其机制的研究进展,大量试验表明诺尼果汁、果汁提取物、叶子和根提取物均有抗肿瘤作用,可通过诱导肿瘤细胞凋亡、激活宿主免疫系统、抑制血管生成、抗氧化、抑制环氧化酶和阻止致癌物-DNA加合物形成等机制来抑制肿瘤生长。本文希望能为研发以诺尼为原料辅助治疗癌症的药物和食品提供参考。%This paper summarizes the progress on anti -tumor effect and its mechanism of Noni over the past decade.Plenty of tests indicated that noni juice , juice extracts, leaf and root extracts have anti -tumor effects.It can inhibit tumor growth through inducing tumor cell apoptosis ,activating host immune system ,inhibi-tion of angiogenesis and cyclooxygenase ,anti -oxidation and blocking carcinogen -DNA adduct formation.This paper provides a reference for the adjuvant therapy of anticancer drugs and food form Noni .

  7. Beverages of lemon juice and exotic noni and papaya with potential for anticholinergic effects.

    Science.gov (United States)

    Gironés-Vilaplana, Amadeo; Valentão, Patrícia; Andrade, Paula B; Ferreres, Federico; Moreno, Diego A; García-Viguera, Cristina

    2015-03-01

    Lemon (Citrus limon (L.) Burm. f.) juice beverages enriched either with noni (Morinda citrifolia L.) (LN) or papaya (Carica papaya L.) (LP), were characterized by HPLC-DAD-ESI/MS(n), the antioxidant capacity was evaluated by (DPPH·), superoxide (O2(·-)), hydroxyl radicals (·OH) and hypochlorous acid (HOCl) assays, and their potential as acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE) inhibitors was also assessed. The fruits are rich in a wide range of bioactive phenolics. Regarding DPPH·, ·OH and HOCl assays, the LP displayed strong activity, and LN was the most active against O2(·-). Concerning cholinesterases, LP was the most active, mainly due to lemon juice contribution. The effect on the cholinesterases was not as strong as in previous reports on purified extracts, but the bioactive-rich beverages offer the possibility of dietary coadjutants for daily consumption of health-promoting substances by adults with aging-related cognitive or physical disorders.

  8. Noni Juice Improves Serum Lipid Profiles and Other Risk Markers in Cigarette Smokers

    Directory of Open Access Journals (Sweden)

    Mian-Ying Wang

    2012-01-01

    Full Text Available Cigarette smoke-induced oxidative stress leads to dyslipidemia and systemic inflammation. Morinda citrifolia (noni fruit juice has been found previously to have a significant antioxidant activity. One hundred thirty-two adult heavy smokers completed a randomized, double blind, placebo-controlled clinical trial designed to investigate the effect of noni juice on serum cholesterol, triglyceride, low density lipoprotein cholesterol (LDL, high density lipoprotein cholesterol (HDL, high-sensitivity C-reactive protein (hs-CRP, and homocysteine. Volunteers drank noni juice or a fruit juice placebo daily for one month. Drinking 29.5 mL to 188 mL of noni juice per day significantly reduced cholesterol levels, triglycerides, and hs-CRP. Decreases in LDL and homocysteine, as well increases in HDL, were also observed among noni juice drinkers. The placebo, which was devoid of iridoid glycosides, did not significantly influence blood lipid profiles or hs-CRP. Noni juice was able to mitigate cigarette smoke-induced dyslipidemia, an activity associated with the presence of iridoids.

  9. Toxicidade reprodutiva da Morinda citrifolia Linn /

    OpenAIRE

    Muller, Juliane Centeno

    2007-01-01

    Orientador : Paulo Roberto Dalsener Dissertação (mestrado) - Universidade Federal do Paraná, Setor de Ciências Biológicas, Programa de Pós-Graduação em Farmacologia. Defesa: Curitiba, 2007 Inclui bibliografia

  10. 诺丽果汁的抗氧化性研究%Antioxidant Activity of the Fresh Juice from Morinda citrifolia

    Institute of Scientific and Technical Information of China (English)

    李奕星; 袁德保; 郑晓燕; 陈娇; 李芬芳; 郑丽丽; 马蔚红; 臧小平; 谭琳

    2013-01-01

    采用分光光度计法,以维他命C(Vc)为对照,考察诺丽果汁的多酚含量及其对1,1-二苯基-2-三硝基苯肼(DPPH)自由基、2,2-联氮双(3-乙基-苯并噻唑-6-磺酸)二铵盐(ABTS)自由基、羟自由基和过氧化氢(H2O2)等的抗氧化活性.结果表明;诺丽果汁中多酚含量较高(1.934 mg/mL),对DPPH自由基、ABTS自由基、羟自由基和过氧化氢等均具有很好的清除活性,且在清除羟自由基能力试验中,其活性显著高于阳性对照(Vc),表明诺丽果汁对人体具有良好的营养保健功效.

  11. Noni 果汁饮料黄酮的分析研究%Determination of content of flavone in fruit juice of Morinda Citrifolia

    Institute of Scientific and Technical Information of China (English)

    孔德霞; 蒋蓉芬; 易美华

    2008-01-01

    目的 研究NONI果汁饮料中黄酮含量的测定方法.方法 采用分光光度法,对Noni果汁中黄酮洗脱剂种类和浓度进行选择和优化.结果 以40%左右的乙醇对Noni果汁中黄酮进行洗脱,分离效果最好,并测试Noni冻干粉、野生Noni发酵果汁、菠萝Noni复合饮料、种植Noni果汁的黄酮含量分别为0.38%、0.11%、0.069%与0.035%.结论 建立的方法简便、易于操作,能准确检测各类Noni果汁饮料中黄酮的含量.

  12. 诺丽、西番莲复合果汁的研制%Preparation of Compound Fruit Juice with Morinda citrifolia and Passiflora coerulea

    Institute of Scientific and Technical Information of China (English)

    阚欢; 蓝增全; 刘惠民

    2009-01-01

    对以诺丽果汁、西番莲果汁为主要原料生产的一种复合果汁进行研究,确定不同果汁的添加比例、生产工艺及产品质量标准.结果表明,当诺丽汁为30%、西番莲果汁为50%时生产的复合果汁综合质量最好.

  13. Generation of Reactive Oxygen and Anti-Oxidant Species by Hydrodynamically-Stressed Suspensions of Morinda citrofolia

    Science.gov (United States)

    The generation of reactive oxygen species (ROS) by plant cell suspension cultures, in response to the imposition of both biotic and abiotic stress, is well-documented. This study investigated the generation of hydrogen peroxide by hydrodynamically-stressed cultures of Morinda citrifolia, over a 5-ho...

  14. Antimutagenic and antirecombinagenic activities of noni fruit juice in somatic cells of Drosophila melanogaster

    Directory of Open Access Journals (Sweden)

    LEONARDO P. FRANCHI

    2013-06-01

    Full Text Available Noni, a Hawaiian name for the fruit of Morinda citrifolia L., is a traditional medicinal plant from Polynesia widely used for the treatment of many diseases including arthritis, diabetes, asthma, hypertension and cancer. Here, a commercial noni juice (TNJ was evaluated for its protective activities against the lesions induced by mitomycin C (MMC and doxorrubicin (DXR using the Somatic Mutation and Recombination Test (SMART in Drosophila melanogaster. Three-day-old larvae, trans-heterozygous for two genetic markers (mwh and flr3 , were co-treated with TNJ plus MMC or DXR. We have observed a reduction in genotoxic effects of MMC and DXR caused by the juice. TNJ provoked a marked decrease in all kinds of MMC- and DXR-induced mutant spots, mainly due to its antirecombinagenic activity. The TNJ protective effects were concentration-dependent, indicating a dose-response correlation, that can be attributed to a powerful antioxidant and/or free radical scavenger ability of TNJ.

  15. Pre-clinical toxicity of Morinda citrifolia Linn. leaf extract

    African Journals Online (AJOL)

    Jane

    2011-10-24

    Oct 24, 2011 ... alternative therapies has raised markedly in a worldwide shape. .... Experimental protocols were approved by the Committee on Animal. Research at the .... In the acute toxicity test, 5 g/kg of AEMC did not cause the death ...

  16. Effect of Morinda citrifolia Fruit Extract Capsule on Total Cholesterol ...

    African Journals Online (AJOL)

    smoking, obesity, lack of exercise and the consumption of high-fat meals .... was found in children and adolescents. Indonesian Health ... explained that physical activity reduces most of ... NCEP-ATP III (National Cholesterol Education Program.

  17. Antioxidative activity and antimicrobial activity in fruit and fermented juice of Morinda citrifolia Linn.%诺丽鲜果与诺丽发酵汁的抗氧化及抑菌活性的对比研究

    Institute of Scientific and Technical Information of China (English)

    龚敏; 苏奎; 邓世明

    2010-01-01

    采用DPPH·自由基法对诺丽鲜果和诺丽发酵汁的抗氧化活性进行了对比研究,研究发现诺丽鲜果的抗氧化活性大于诺丽发酵汁.还采用了滤纸片法对诺丽鲜果及诺丽发酵汁的抑菌活性进行对比研究,研究发现诺丽发酵汁的抑菌活性高于诺丽鲜果的抑菌活性.

  18. GC-MS analysis of volatile components in fruit and fermented juice of Morinda citrifolia%诺丽鲜果与诺丽发酵汁的挥发性成分的对比研究

    Institute of Scientific and Technical Information of China (English)

    龚敏; 符文英; 周静; 邓世明

    2009-01-01

    采用气相色谱-质谱联用方法对诺丽鲜果和诺丽发酵汁中的挥发性成分进行检测和分析.结果显示,诺丽鲜果与诺丽发酵汁中的挥发性成分主要是脂肪酸和酯类.而在诺丽鲜果中脂肪酸是主要的挥发性成分,占总挥发性成分43.34%,十八烯酸就占了20.34%;在诺丽发酵汁中主要的挥发性成分是酯类,占了总挥发性成分的74.92%,而2-羟基丙酸丁酯就占了64.36%.

  19. Determination of Free Water-soluble Vitamins in Cook Noni (Morinda citrifolia) Juice by HPLC%HPLC法测定库克Noni果汁中游离态水溶性维生素

    Institute of Scientific and Technical Information of China (English)

    李昌; 谢明勇; 陈军辉; 聂少平; 黄优生

    2006-01-01

    采用HPLC法测定Noni果汁中游离态的抗坏血酸(Vc)、硫胺素(VB1)和烟酰胺(VB5),其测定条件为:色谱柱为Alltima C18柱,流动相为甲醇-0.01%磷酸(3:7,V/V),流速为1.0 mL/min,进样量为20 μL,柱温为室温,采用二极管阵列检测器在251 nm下检测.在上述色谱测定条件下三种维生素能得到良好分离.结果表明,该方法灵敏度高,定量准确,重现性好,回收率高.所测Noni果汁中三种维生素的含量分别为:Vc 0.354 mg/mL,VB1 0.066 3 mg/mL,VB5 0.131 mg/mL.说明Noni果汁中三种必需维生素含量丰富,可作为维生素缺乏的营养补充剂;所建立的测定方法可作为一种Noni果汁生产质量控制方法.

  20. Content and Relative Molecular Weight Determination of the Polysaccharide in Cook Noni(Morinda citrifolia) Juice%库克Noni果汁多糖含量及分子量测定

    Institute of Scientific and Technical Information of China (English)

    李昌; 谢明勇; 聂少平; 万茵

    2007-01-01

    建立测定库克Noni果汁中多糖含量及分子量的方法.通过醇沉法分离Noni果汁多糖,进一步分离纯化得到均一多糖组分;用精制Noni果汁多糖测得该多糖对葡萄糖的换算因子,对多糖含量进行定量测定;并通过SE-HPLC(Size Exclusion-High Performance Liquid Chromatography)法测定了该多糖的相对分子量.结果表明,多糖含量测定方法简便可行,供试液在4 h内显色稳定,重现性较好,平均回收率为99.3%,RSD为1.93%(n=3);测得该多糖的相对分子量为1140 KDa.

  1. 诺丽鲜果与诺丽发酵汁化学成分研究%Studies on the Chemical Constituents of Fruit and Fermented Juice of Morinda citrifolia L.

    Institute of Scientific and Technical Information of China (English)

    龚敏; 苏奎; 杨先会; 符文英; 邓世明

    2011-01-01

    目的 对诺丽鲜果与诺丽发酵汁化学成分进行对比研究.方法 应用硅胶柱层析、葡聚糖凝胶柱层析、结晶等方法对诺丽鲜果及诺丽发酵汁的化学成分进行了分离纯化,并结合现代波谱技术对诺丽鲜果和诺丽发酵汁的化学成分进行了鉴定.结果 从诺丽鲜果中分离并鉴定出了β-谷甾醇、异东莨菪辛内脂、熊果酸、熊果酸甲酯共4个化合物.而从诺丽发酵汁中分离鉴定出了β-谷甾醇、双-[(2-乙基)乙基]邻苯二甲酸酯、东莨菪亭内脂共3个化合物.结论 诺丽鲜果与诺丽发酵汁中共有的化学成分为β-谷苗醇.

  2. The Genetics of Resistance to Morinda Fruit Toxin During the Postembryonic Stages in Drosophila sechellia.

    Science.gov (United States)

    Huang, Yan; Erezyilmaz, Deniz

    2015-10-01

    Although a great deal has been learned regarding the genetic changes that give rise to adaptation in bacteria and yeast, an understanding of how new complex traits arise in multicellular organisms is far less complete. Many phytophagous insect species are ecological specialists that have adapted to utilize a single host plant. Drosophila sechellia is a specialist that utilizes the ripe fruit of Morinda citrifolia, which is toxic to its sibling species, D. simulans. Here we apply multiplexed shotgun genotyping and QTL analysis to examine the genetic basis of resistance to M. citrifolia fruit toxin in interspecific hybrids. We identify a locus of large effect on the third chromosome (QTL-IIIsima) in the D. simulans backcross that was not detected in previous analyses. We also identify a highly significant QTL of large effect on the X chromosome, QTL-Xsim. Additional smaller-effect loci were also identified in the D. simulans and D. sechellia backcrosses. We did not detect significant epistasis between loci. Instead, our analysis reveals large and smaller-effect loci that contribute to M. citrifolia resistance additively. The additive effect of each locus suggests that partial resistance to lower levels of M. citrifolia toxin could be passed through introgression from D. sechellia to D. simulans in nature. The identification of the major effect loci, QTL-IIIsima and QTL-Xsim, is an important step toward identifying the molecular basis of adaptation in a multicellular organism.

  3. Ability of Lactobacillus plantarum JR64 isolated from noni juice in lowering Cholesterol in vivo

    Directory of Open Access Journals (Sweden)

    Lanjar Sumarno

    2011-07-01

    Full Text Available Recently public’s attention to the importance of healthy food increases rapidly. Probiotic based food exploiting lactic acid bacteria is among the healthy food. Lactobacillus plantarum JR64 isolate from Morinda citrifolia fruit was assessed for its probiotic in-vivo by using Wistar  Rat. The purpose of this research was to study the ability of probiotic Lactobacillus plantarum JR64 in lowering serum LDL (Low Density Lipoprotein of Wistar Rat. Twenty Rats were grouped into 4, each group consisted of 5 Rats. First Group was a negative control  given standard normal diet of 20 gr/day plus aquadest. Second Grup was a positive control given cholesterol normal diet 20 gr /day plus Propil Tio Urasil (PTU 60 mg/kg body weight/day. Third Group was supplemented with normal diet 20 gr /day plus Propil Tio Urasil (PTU 60 mg  kg body weight /day and  1012 CFU Lactobacillus plantarum JR64. Fourth Group was the same as third Group unless the probiotic using commercial probiotic Lactobacillus bulgariccus at 1012 CFU. Blood samples were withdrawn for measurement of total cholesterol, triglyceride, High Density Lipoprotein (HDL, and Low Density Lipoprotein (LDL cholesterol every week and measured by using spectrophotometer with 546 nanometers wavelength. The results show that probiotic Lactobacillus plantarum JR64 isolated from noni juice significantly (p < 0,01 reduce Low Density Lipoprotein (LDL and Triglyceride in vivo  and tend to reduce High Density Lipoprotein (HDL and total cholesterol.

  4. Desponte e embebição em sementes de noni (Morinda citrifolia L. Lopping and soaking in seeds of noni (Morinda citrifolia L.

    Directory of Open Access Journals (Sweden)

    K.P Oliveira

    2011-01-01

    Full Text Available O trabalho teve como objetivo testar a germinação em sementes de noni sob diferentes tempos de embebição, com e sem o desponte das sementes. Para isso, instalou-se um experimento em casa de vegetação no delineamento inteiramente casualizado com 10 tratamentos e 4 repetições de 25 sementes , sendo constituídos por: 1. Testemunha; 2. Sementes com desponte do lado oposto ao embrião; 3. Sementes intactas com 12 horas de embebição; 4. Sementes intactas com 24 horas de embebição; 5. Sementes intactas com 36 horas de embebição; 6. Sementes intactas com 48 horas de embebição; 7. Sementes com desponte com 12 horas de embebição; 8. Sementes com desponte com 24 horas de embebição; 9. Sementes com desponte com 36 horas de embebição e 10. Sementes com desponte com 48 horas de embebição. As características avaliadas foram: porcentagem de emergência, Índice de velocidade de emergência, altura de plântulas, comprimento de raiz e peso de massa seca das plântulas aos 46 dias após a instalação do experimento. Houve diferença significativa entre os diferentes tempos de embebição e o tratamento 10 (Desponte com 48 hs de embebição que obteve os melhores resultados para a percentagem de emergência, índice e velocidade de emergência e peso seco. Quanto a altura de plântula, o tratamento 1 (Testemunha foi o maior, enquanto no tratamento 2 (desponte se verificou o maior comprimento de raiz. Embora não tenha diferido da testemunha, o melhor tratamento foi o uso do desponte + 48 horas de embebição.The study aimed to test the germination of seeds of noni under different soaking times, with and without the seed emerges. For this, an experiment was installed in a greenhouse in a completely randomized design with 10 treatments and 4 replicates of 25 seeds, being composed of: 1. Witness 2. Seeds with the blunt side opposite the embryo 3. Intact seeds with 12 hours of soaking, 4. Intact seeds with 24 hours of soaking, 5. Intact seeds with 36 hours of soaking, 6. Intact seeds with 48 hours of soaking; 7. Seeds with cutting with 12 hours of soaking; 8. Seeds with cutting with 24 hours of soaking; 9. Seeds with cutting with 36 hours of soaking and 10. Seeds with cutting with 48 hours of soaking. The characteristics evaluated were: percentage of emergence, speed of emergence index, seedling height, root length and weight of the dry mass of seedlings 46 days after the experiment. There were significant differences between the different times of soaking and treatment 10 (cutting with 48 hours of soaking who obtained the best results for the percentage of emergency and emergency speed index and dry weight. The height of the seedling, one treatment (control was the highest, while in treatment 2 (blunt registered the largest root length. Although the witness did not differ, the best treatment was the use of blunt + 48 hours of soaking.

  5. ACTIVIDADE ANTIMICROBIANA E EFEITO COMBIANDO SOBRE DROGAS ANTIFUNGICAS Y ANTIBACTERIANAS DO FRUTO DE Morinda citrifolia L

    National Research Council Canada - National Science Library

    Saulo Relison TINTINO; Abel Alves de Carvalho NETO; Irwin R A MENEZES; Cícera Datiane de M OLIVEIRA; Henrique D M COUTINHO

    2015-01-01

    .... Possui diversos metabólitos primários e secundários, principalmente flavonóides, triterpenóides e alcalóides. O presente trabalho visou testar o potencial antimicrobiano e modulador dos extratos do seu fruto...

  6. Biochemistry and Cell Wall Changes Associated with Noni (Morinda citrifolia L.) Fruit Ripening.

    Science.gov (United States)

    Cárdenas-Coronel, Wendy G; Carrillo-López, Armando; Vélez de la Rocha, Rosabel; Labavitch, John M; Báez-Sañudo, Manuel A; Heredia, José B; Zazueta-Morales, José J; Vega-García, Misael O; Sañudo-Barajas, J Adriana

    2016-01-13

    Quality and compositional changes were determined in noni fruit harvested at five ripening stages, from dark-green to thaslucent-grayish. Fruit ripening was accompanied by acidity and soluble solids accumulation but pH diminution, whereas the softening profile presented three differential steps named early (no significant softening), intermediate (significant softening), and final (dramatic softening). At early step the extensive depolymerization of hydrosoluble pectins and the significantly increment of pectinase activities did not correlate with the slight reduction in firmness. The intermediate step showed an increment of pectinases and hemicellulases activities. The final step was accompanied by the most significant reduction in the yield of alcohol-insoluble solids as well as in the composition of uronic acids and neutral sugars; pectinases increased their activity and depolymerization of hemicellulosic fractions occurred. Noni ripening is a process conducted by the coordinated action of pectinases and hemicellulases that promote the differential dissasembly of cell wall polymers.

  7. Noni puree (Morinda citrifolia) mixed in beef patties enhanced color stability.

    Science.gov (United States)

    Tapp, W Nathan; Yancey, Janeal W S; Apple, Jason K; Dikeman, Michael E; Godbee, Richard G

    2012-06-01

    Ground beef, mixed with 0, 2, 4, and 6% Noni puree, was formed into 150-g patties, aerobically packaged, and displayed in retail for 5d. After 2 and 3d, patties with higher concentrations of Noni were perceived as redder and less discolored (P<0.05) by visual panelists. Noni patties were found to have greater (P<0.05) a* values than controls, even though all patties became less red during display. After 3 and 5d of retail display, patties with higher concentrations of Noni puree also had lower TBARS (were less oxidized; P<0.05). In fresh taste panels, panelists perceived the patties to have less beef flavor and greater incidence of off-flavors (P<0.05) as Noni puree concentration increased. The potential of Noni puree to improve the color stability and shelf life of fresh ground beef is very promising, but the flavors produced by the addition of Noni in ground beef may be detrimental to its use.

  8. Evaluation of noni (Morinda citrifolia) volatile profile by dynamic headspace and gas chromatography-mass spectrometry

    OpenAIRE

    Sousa, A.; M. A. SOUZA NETO; GARRUTI, D. dos S.; Sousa, J. A.; BRITO, E. S. de

    2010-01-01

    Noni is a fruit that has interested the scientific community due to its medicinal and functional activities. Different products that contain noni are already in the market, but their consumption could be impaired by their distinctive unpleasant aroma and flavor. The aim of this work was to evaluate the noni pulp volatile profile by dynamic headspace and gas chromatography-mass spectrometry. Thirty seven volatile compounds were detected, mainly alcohols (63.3%), esters (26.9%), cetones (7.4%),...

  9. Fruit Juice.

    African Journals Online (AJOL)

    The Effect of Homogenization, Stabilizer and Amylase Treatment on Viscosity of Passion. Fruit Juice. ... viscosity during storage of sweetened, pasteurized passion fruit juice were investigated. .... minutes after which the temperature was.

  10. Modulatory Effect of Morinda lucida Aqueous Stem Bark Extract on ...

    African Journals Online (AJOL)

    Dr Olaleye

    analyses of the aqueous stem bark of Morinda lucida were conducted using standard procedures. Results showed that the ... disease and stroke, neuropathy, nephropathy, cataracts, .... β-cells destruction (Gupta et al., 1984). Fasting blood ...

  11. Volatile compounds in noni (Morinda citrifolia L. at two ripening stages Compostos voláteis em noni (Morinda citrifolia L. em dois estágios de maturação

    Directory of Open Access Journals (Sweden)

    Jorge Antonio Pino

    2010-03-01

    Full Text Available The volatile components of noni at two ripening stages were isolated by headspace solid-phase microextraction using 65 µm Polydimethylsiloxane-Divinylbenzene (PDMS/DVB fibers and analyzed using GC/MS. Both maturation stages had several compounds in common. Ninety-six compounds were identified, from which octanoic acid ( 70% of total extract and hexanoic acid ( 8% of total extract were found to be the major constituents. Due to noni maturation, octanoic acid, decanoic acid and 2E-nonenal decreased their concentrations, while some esters (methyl hexanoate, methyl octanoate, ethyl octanoate and methyl 4E-decenoate, which their fruity odor notes, increased their contents. Two unsaturated esters, reported for the first time in this fruit, 3-methyl-3-buten-1-yl hexanoate and 3-methyl-3-buten-1-yl octanoate, significantly decreased their concentration in the ripe to over-ripe fruits.Los compuestos volátiles del noni en los dos estados de madurez se aislaron mediante microextracción en fase sólida por headspace con fibras de 65 mm Polidimetilsiloxano-Divinilbenceno (PDMS/DVB y se analizaron por cromatografía de gases/espectrometría de masas. Ambos estados de madurez poseen numerosos constituyentes en común. Se identificaron 96 compuestos, de los cuales el ácido octanoico ( 70% del extracto total y ácido hexanoico ( 8% del extracto total fueron los componentes mayoritarios. Debido a la maduración del noni, el ácido octanoico, ácido decanoico y 2E-nonenal disminuyeron su contenido, mientras que algunos ésteres (hexanoato de metilo, octanoato de metilo, octanoato de etilo y 4E-decenoato de metilo, con sus olororosas notas frutales, incrementaron la concentración. Dos ésteres insaturados, reportados por primera vez en esta fruta, hexanoato de 3-metil-3-buten-1-ilo y octanoato de 3-metil-3-buten-1-ilo, disminuyeron significativamente su concentración al pasar la fruta del estado maduro al sobremaduro.

  12. The impact of convective drying on the color, phenolic content and antioxidant capacity of noni (Morinda citrifolia L.

    Directory of Open Access Journals (Sweden)

    Ana Isabel MIRELES-ARRIAGA

    Full Text Available Abstract The effect of thin-layer drying temperature on color, phenolic content and antioxidant capacity of noni slices was investigated. Noni slices were air-dried at 50, 60 and 70 °C under natural convection conditions. Drying curves were fitted to thin-layer drying models in order to evaluate the drying characteristics of the product. In addition, an unsteady-state diffusion equation was numerically solved considering both product shrinkage and a variable mass Biot number in boundary condition to accurately estimate water diffusivity in a process with external resistance to mass transfer. Results revealed that the Aghbashlo model accurately reproduced the experimental behavior. As expected, water diffusivities, corrected for shrinkage, increased with the use of higher drying temperatures, with values in the range of 1.80-3.19 to ×10-9 m2/s. On the other hand, while drying caused a reduction in product lightness (24-31% and total phenolic content (20-28%, the antioxidant capacity of dried noni was high (82-93% DPPH inhibition, which is advantageous for the further storage or processing of this fruit.

  13. The planting and use of Morinda citrifolia%海巴戟天的栽培及其利用

    Institute of Scientific and Technical Information of China (English)

    甘炳春; 何明军

    2004-01-01

    介绍了药用植物海巴戟天的自然分布、形态特征、栽培管理和病虫害的防治技术,报道了海巴戟天的药用历史和现代医学研究结果以及产品开发现状,提出种植开发利用建议.

  14. Effect of fermented noni leaf (Morinda citrifolia L. in diets on cholesterol content of broiler chicken carcass

    Directory of Open Access Journals (Sweden)

    Erman Syahruddin

    2011-12-01

    Full Text Available Chicken meat is very nutritious. It is sometimes blamed to cause strock attack and coronary heart disease in human, because of high fat and cholesterol contents in the chicken meat. Therefore, the aim of this experiment is to evaluate the effect of fermented noni leaf levels in diets on the cholesterol content of broiler chicken carcass. The experiment was based on completely randomized design with eight experimental diets containing 0, 3, 6, 9, 12, 15, 18 and 21% of fermented noni leaf powder. All diets were formulated to contain 22% crude protein and 3000 kcal/kg. Each treatment had three replicates with ten chickens per replicate. Two hundred and forty day old unsex broiler chicks Arbor Acress were fed ad lib. for eight weeks and then sacrificed. Feed consumption, body weight gain, feed conversion ratio, and cholesterol content of carcass were taken as variable responses. Data were analyzed based on analysis of variance and orthogonal comparisons. Results showed that feed consumption, daily weight gain, FCR and carcass content were not affected by the levels of fermented noni leaf in the diet. However, cholesterol content of broiler carcass was significantly (P < 0.05 affected by the dietary treatments. Cholesterol content of the carcass was reduced processed 26.18% 73.06 to 53.76 mg/100g mg/100g chicken meat. The lowest cholesterol level was obtained by feeding the chickens with diets containing 21% fermented noni leaf.

  15. 诺丽果肉化学成分研究%Chemical constituents of the sarcocarp of Morinda citrifolia

    Institute of Scientific and Technical Information of China (English)

    何其伟; 吴培云; 梁益敏; 项晨; 杜子伟; 王刚

    2012-01-01

    目的 研究诺丽果肉的化学成分.方法 利用硅胶、RP-18、Sephadex LH-20等柱色谱手段进行分离,根据理化性质和波谱分析鉴定化合物的结构.结果 从乙醇提取物的乙酸乙酯部分和正丁醇部分中分离得到12个化合物,分别鉴定为β-谷甾醇(1)、乌苏酸(2)、莨菪亭(3)、邻苯二甲酸二甲酯(4)、4-epi-dunnisinin (5)、(24S) -ergost7-en-3β-ol (6)、19-羟基-乌苏酸(7)、胡萝卜苷(8)、丁二酸(9)、1-O-(3'-methylbut-3'-enyl)-β-D-glucopyranose (10)、borreriagenin (11)、3-methylbut-3-enyl-6-O-β-D-glucopyranosyl-β-D-glucopyranoside( 12).结论 化合物4为首次从该属植物中分离得到.

  16. Nutraceutical properties of Thai

    Directory of Open Access Journals (Sweden)

    Chareonchai Jeamchanya

    2005-08-01

    Full Text Available Morinda citrifolia Linn. is known in Thailand as "Yor" and commonly called "Noni" in Hawaii, Tahiti and USA. This paper reports the results of chemical analysis of Thai "Yor" (M. citrifolia juice extract. The juice from "Yor" fruit, was found to be high in vitamin C, vitamin B-complex contents (HPLC method, and was also high in anti-oxidants, anthraquinones, flavonoids, saponins and scopoletin (TLC method. Sodium, potassium, calcium, iron and selenium contents of the juice (AAS method are also reported. All the results of Thai "yor" juice are comparable to those of the commercially distributed 'Noni' juice. The Thai "Yor" juice was free of microorganisms and could safely be consumed in the recommended amount of 30 ml/day. The results of this study only apply to the Thai "Yor" juice preparation. The commercial "Noni" juice may be processed differently and may contain other ingredients.

  17. Morinda lucida reduces contractility of isolated uterine smooth ...

    African Journals Online (AJOL)

    Morinda lucida reduces contractility of isolated uterine smooth muscle of ... (OXY; 10-5–10-2 mol/L), acetylcholine (ACh; 10-9-10-5 mol/L) and M. lucida extract ... by L-NAME suggesting that the action of the compound on uterine muscle is not ...

  18. Antispermatogenic activity of Morinda lucida extract in male rats

    Institute of Scientific and Technical Information of China (English)

    Yinusa Raji; Olumide S. Akinsomisoye; Toyin M. Salman

    2005-01-01

    Aim: To investigate the effect of Morinda lucida Benth (Rubiaceae) on the reproductive activity of male albino rats.respectively. The control rats received the vehicle. All the treated rats had corresponding recovery groups. At the end of each experimental period, animals were killed and organ weights, sperm characteristics, serum testosterone levels,histology of the testes and fertility were assessed. Results: Morinda lucida leaf extract did not cause any changes in body and somatic organ weights, but significantly increased the testis weight (P < 0.05). The sperm motility and viability, and the epididymal sperm counts of rats treated for 13 weeks were significantly reduced (P < 0.05). Sperm morphological abnormalities and serum testosterone levels were significantly increased (P < 0.05). There were various degrees of damage to the seminiferous tubules. The extract reduced the fertility of the treated rats by reducing the litter size. Reversal of these changes, however, occurred after a period of time. Conclusion: The extract of Morinda lucida has reversible antispermatogenic properties.

  19. Grapefruit Juice and Statins.

    Science.gov (United States)

    Lee, Jonathan W; Morris, Joan K; Wald, Nicholas J

    2016-01-01

    We determined the validity of current medical advice to avoid grapefruit juice consumption while taking 3 widely used statins. A daily glass of grapefruit juice increases blood levels of simvastatin and lovastatin by about 260% if taken at the same time (about 90% if taken 12 hours apart), and atorvastatin by about 80% (whenever taken). Simvastatin 40 mg, lovastatin 40 mg, and atorvastatin 10 mg daily reduce low-density lipoprotein (LDL) cholesterol levels in a 60-year-old man with an LDL cholesterol of 4.8 mmol/L by 37%, reducing ischemic heart disease risk by 61%. When simvastatin or lovastatin are taken at the same time as grapefruit juice, the estimated reduction in LDL cholesterol is 48%, and in heart disease is 70%. If the juice is taken 12 hours before these statins, the reductions are, respectively, 43% and 66%, and for atorvastatin, 42% and 66%. The increased rhabdomyolysis risk from grapefruit juice consumption due to the increased effective statin dose is minimal compared with the greater effect in preventing heart disease. Grapefruit juice should not be contraindicated in people taking statins.

  20. 21 CFR 146.185 - Pineapple juice.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 2 2010-04-01 2010-04-01 false Pineapple juice. 146.185 Section 146.185 Food and... CONSUMPTION CANNED FRUIT JUICES Requirements for Specific Standardized Canned Fruit Juices and Beverages § 146.185 Pineapple juice. (a) Identity. (1) Pineapple juice is the juice, intended for direct consumption...

  1. 21 CFR 146.135 - Orange juice.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 2 2010-04-01 2010-04-01 false Orange juice. 146.135 Section 146.135 Food and... CONSUMPTION CANNED FRUIT JUICES Requirements for Specific Standardized Canned Fruit Juices and Beverages § 146.135 Orange juice. (a) Orange juice is the unfermented juice obtained from mature oranges of the...

  2. 21 CFR 146.114 - Lemon juice.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 2 2010-04-01 2010-04-01 false Lemon juice. 146.114 Section 146.114 Food and... CONSUMPTION CANNED FRUIT JUICES Requirements for Specific Standardized Canned Fruit Juices and Beverages § 146.114 Lemon juice. (a) Identity—(1) Description. Lemon juice is the unfermented juice, obtained by...

  3. Cranberry juice: effects on health

    Science.gov (United States)

    Cranberries have long been used as a part of traditional and folk medicine. Most cranberry juice is consumed as a product containing 27% v/v with sweeteners derived from other fruit juices or other sweeteners. Cranberry juice contains a rich profile of phenolic compounds, especially proanthocyanidin...

  4. 四种处理方法对诺丽(Morinda citrifolia Linn.)叶片褐化影响的研究

    Institute of Scientific and Technical Information of China (English)

    潘晓晴; 吴田; 蓝增全; 吴腾超; 陶燕蓝

    2013-01-01

    本研究以诺丽叶片为研究材料,通过柠檬酸处理、热激法处理、CaC12处理和采用较嫩叶片比较法处理,并使用75%的酒精脱色,与对照组材料对比,观察叶片的褐化程度.结果表明:采用0.2%柠檬酸溶液处理过的叶片褐化程度最轻;采用90℃左右热水中烫5~7s处理的叶片褐化程度次之;采用6%CaCl2溶液中浸泡15min处理的叶片褐化程度与对照组的叶片褐化程度相当;而采用茎尖下第一对叶片的褐化程度最严重.

  5. The Seeds Structure and Germination Test of Morinda citrifolia Linn.%海巴戟的种子结构及发芽试验

    Institute of Scientific and Technical Information of China (English)

    邢诒旺; 符懋修; 李承武; 符文英

    2007-01-01

    对海巴戟种子的形态和结构进行了观察研究,并从环境温度、播种基质、物理处理等方面,探讨了其种子的萌发特性及生理.试验结果表明:海巴戟种子的萌发特性与其结构特点有关;高温能显著提高其种子的发芽率、发芽势和发芽指数,且高温条件下变温更有利于种子的萌发,最适宜的发芽温度是28~35 ℃,28℃和33℃则是发芽的最佳变温;海巴戟种子本身存在着某种抑制萌发的特殊物质,但在土壤中这种拟制萌发的状态可被打破而诱导萌发;透气和保水性能良好的播种基质对海巴戟种子的萌发有利,以河沙加泥炭土作为播种基质的海巴戟种子,发芽率最高,达77.0%,其相应的发芽势和发芽指数也最高,分别为63.5%和12.532;温水处理对海巴戟种子的萌发具有明显的促进作用,能提高它的出苗整齐度,且其发芽率和发芽势均最高,分别达74.0%和62.5%,其相应的发芽指数为12.271.

  6. 海巴戟天的离体快速繁殖(简报)%Rapid Propagation of Morinda citrifolia Linn. in vitro

    Institute of Scientific and Technical Information of China (English)

    陈雄庭; 张秀娟; 王颖; 吴坤鑫

    2007-01-01

    从多果无病虫害的母树中选取的幼嫩侧枝,经表面消毒后切取侧芽并接种到MS基本培养基,附加蔗糖浓度30 g/L,BA 2 mg/L,NAA 0.1 mg/L初代培养基上,培养40 d后,侧芽萌发率达80%以上.增殖培养基与初代培养基相同,以40~50 d为一增殖周期,增殖系数达2~3倍.当新芽增殖到足够数量时,切取2~3 cm的新芽接种到1/2MS大量元素,全量微量元素,蔗糖浓度为30 g/L,IBA 1 mg/L,活性炭1 g/L的生根培养基生根培养基上,生根率高达100%,植株在沙床的移栽成活率均达95%以上.30 cm高的植株便可种植到大田.

  7. Comparison of antimicrobial efficacy of propolis, Morinda citrifolia, Azadirachta indica, triphala, green tea polyphenols and 5.25% sodium hypochlorite against Enterococcus fecalis biofilm

    Directory of Open Access Journals (Sweden)

    Paridhi Garg

    2014-01-01

    Full Text Available Introduction: Endodontic infections are polymicrobial in nature. Enterococcus fecalis is the most common micro-organism isolated from failed endodontic cases. The constant increase in antibiotic resistant strains and side effects caused by synthetic drugs has prompted researchers to look for herbal alternatives since the gold standard for irrigation i.e., sodium hypochlorite (NaOCl has many disadvantages. Objective: The present study was aimed to explore newer irrigation solutions, which would probably be as effective as NaOCl. Materials and Methods: Extracted human single rooted premolar teeth were biomechanically prepared, vertically sectioned, placed in tissue culture wells exposing the root canal surface to E. fecalis is grown on Mueller Hinton agar plates to form a biofilm for 6 weeks. At the end of 6 th week, all seven groups were treated with 3 ml of test solutions and control for 10 minutes and evaluated for E. fecalis growth and number of colony forming units. Results: Propolis, NaOCl and triphala showed no statistically significant difference, whereas all the other inter-group differences were statistically significant (Tukey′s honest significant difference (HSD (P < 0.001. Conclusion: Propolis and triphala were found to be as efficacious as NaOCl. The use of herbal alternatives as root canal irrigation solutions might prove to be advantageous considering several unfavorable properties of NaOCl.

  8. CARACTERIZAÇÃO QUÍMICA E FÍSICO-QUÍMICA DA POLPA DO NONI (MORINDA CITRIFOLIA CULTIVADO NO ESTADO DO CEARÁ

    Directory of Open Access Journals (Sweden)

    Antonia Alaís da Silva CORREIA

    2011-12-01

    Full Text Available O presente trabalho teve por objetivo avaliar as características químicas e físico-químicas da polpa do noni cultivado no estado do Ceará. Frutos maduros provenientes de um plantio localizado na cidade Horizonte (Ceará-Brasil foram despolpados e a polpa extraída, embalada em sacos de polietileno, selados e armazenados a -20°C para posteriores análises. Foi determinada a composição centesimal: umidade (91,91%, carboidratos (0,63%, lipídeos totais (0,08%, proteínas (1,06%, cinzas (6,32%; fibra (1,76%, valor energético (30,25kcal.100g-1 e análises químicas e físico-químicas: pH (4,25, sólidos solúveis totais (9,2°Brix, acidez total titulável (0,63g.100g-1, SST/ AAT (14,66, vitamina C (122,54mg de AA.100g-1, açúcares totais (5,45g.100g-1, açúcares redutores (5,32g.100g-1; fenólicos totais (216,67mg de EAG.100g-1, antioxidante (6,27μmol. TEAC g-1, atividade de água (0,93; (AIR resíduo insolúvel em álcool (2,8g.100g-1, pectina (1,14g.100g-1, hemicelulose (0,25g.100g-1 e celulose +lignina (0,45g.100g-1. Os resultados mostram que a polpa de noni possui um baixo teor de proteína e lipídeo, é rica em carboidratos e apresenta uma significativa quantidade de antioxidantes, com destaque para a vitamina C e compostos fenólicos.

  9. Lipid profiles of blood serum and fatty acid composition of meat of hybrid duck fed diet supplemented with Noni (Morinda citrifolia fruit meal

    Directory of Open Access Journals (Sweden)

    David Kurniawan

    2015-09-01

    Full Text Available Noni fruit is a medicinal plant with biological activity like antioxidant that could potentially be used as a feed additive in poultry. This research investigated the effect of noni fruit powder as feed additive on lipid profiles of blood and meat fatty acid compositions of meat of hybrid duck. One hundred twenty 2-week-old hybrid ducks crossing between Peking and Khaki Campbell duck were subjected. They were randomly allotted to 24 experimental units. Each experimental unit was 70x80x40 cm in size and it was used for 5 ducks up to they reached 56 days of age. Each unit was equipped with waterer and feeder. The ducks were raised on litter-type floor. The basal experimental diet was formulated according to the standards of National Research Council (1994. The method used for this study was experimental with 4 different treatments in 6 replications. The treatments were as follow: P0: basal feed without supplementation of noni fruit powder as control; P1: basal feed + 1 % noni fruit powder; P2: basal feed + 2 % noni fruit powder; P3: basal feed + 3 % noni fruit powder. Data were analyzed by one-way of Completely Randomized Design ANOVA and if there was significant effect followed by Duncan’s Multiple Range Test. Result showed that using noni fruit powder as feed additive had no significant effect (P>0.05 on lipid profiles of blood and fatty acid composition of meat.

  10. 诺丽种子的生活力测定与发芽试验%Test on Seed Viability and Germination of Morinda citrifolia

    Institute of Scientific and Technical Information of China (English)

    李青红; 蓝增全; 李法营

    2010-01-01

    [目的]研究影响诺丽种子萌发的因素.[方法]以诺丽种子为材料,用四唑染色法测定种子的生活力,采用8种不同的处理方法进行种子发芽试验.[结果]诺丽种子的平均生活力为17%,8种处理中,aBE(不消毒、浸种、剪口)和ABD(消毒、浸种、毒口)对诺丽种子发芽影响显著,其平均发芽率分别为13.3%、12%;平均发芽势分别为5.7%、7.7%.单独采用消毒、浸种、剪口处理对诺丽种子发芽影响不大.[结论]诺丽种子萌发困难受到多个因素的影响,而非单一因素作用的结果,温水浸种并剪口处理促进诺丽种子的萌发,能提高它的发芽整齐度.

  11. Água salina e biofertilizante bovino na produção de frutos e alocação de biomassa em noni (Morinda citrifolia L.

    Directory of Open Access Journals (Sweden)

    A.G.L. SOUTO

    2015-06-01

    Full Text Available RESUMO:Têm sido evidenciado que os compostos orgânicos exercem ação mitigadora dos efeitos tóxicos dos sais às plantas, promovendo maior crescimento e desenvolvimento em solos com excesso de sais. Nessa direção, um experimento foi desenvolvido para avaliar os efeitos do biofertilizante bovino na produção de frutos e alocação de biomassa pelos diversos órgãos de plantas de noni, irrigadas com águas salinas. Os tratamentos foram distribuídos em blocos ao acaso, com quatro repetições e duas plantas por parcela, adotando o arranjo fatorial 5 x 2, correspondente a salinidade das águas de irrigação de 0,5; 1,5; 3,0; 4,5; 6,0 dS m-1, em substratos sem e com biofertilizante bovino, aplicado uma única vez, em volume correspondente a 10% do volume do substrato, um dia antes da instalação do experimento. As variáveis avaliadas foram: salinidade do solo, expressa pela condutividade elétrica do extrato de saturação do solo; número de frutos; massa média de fruto; produção de frutos por planta; e alocação de biomassa pelas raízes, caules, folhas e frutos. O aumento da salinidade da água de irrigação elevou o caráter salino do solo desde "não salino" para "fortemente salino" e, inibiu a produção de frutos e a acumulação de biomassa seca das plantas de noni em geral, mas, com maior intensidade nos tratamentos sem o insumo orgânico. O biofertilizante estimula o crescimento e a produção de plantas de noni cultivadas sob irrigação com águas de alta salinidade.

  12. Determination of Mineral Elements in Morinda citrifolia L.%海巴戟中7种矿物质元素的测定

    Institute of Scientific and Technical Information of China (English)

    聂风琴; 于文辉; 廖丹; 刘伯宏; 李彩凤; 符文英

    2015-01-01

    利用空气-乙炔火焰原子吸收光谱法对海巴戟果肉、叶、果实冻干粉及发酵果汁中的钾、钙、钠、镁、铁、锌、锰等7种重要矿物质元素进行测定.结果表明:实验回收率为94% ~ 107.2%;海巴戟中钾、钙、锌、铁、锰元素含量丰富;海巴戟果肉中钾元素含量高,为24 215.81 μg·g-1,钠元素含量相对较低,仅为789.40 μg·g-1,是一种典型的低钠高钾保健食品,具有良好的保健养生价值.除钾元素外,叶中其他元素含量均高于果肉中的,尤其是微量元素铁、锌、锰的含量.海巴戟产品冻干粉中的7种元素含量与诺丽果肉中的接近.海巴戟发酵果汁与同一品种海巴戟果肉中的矿物质元素含量成一定比例,7种矿物质元素均能够满足人体需求.

  13. Genome Sequence of Paenibacillus polymyxa Strain CICC 10580, Isolated from the Fruit of Noni (Morinda citrifolia L.) Grown in the Paracel Islands.

    Science.gov (United States)

    Xu, Youqiang; Liu, Yang; Yao, Su; Li, Jinxia; Cheng, Chi

    2014-01-01

    Noni is a plant reported to have nutritional and therapeutic properties. Paenibacillus polymyxa CICC 10580 is a strain that was isolated from the fruit of noni and showed comprehensive antagonistic activity against many pathogens. Its genome was sequenced and assembled (6.10 Mb). The coding sequences (CDSs) correlated with antagonistic activity were annotated.

  14. 海巴戟良种‘万维1号’%An Elite Variety of Noni(Morinda citrifolia) ‘Wanwei No. 1’

    Institute of Scientific and Technical Information of China (English)

    邢诒旺; 符传贤; 林道哲; 李琼义; 符文英

    2014-01-01

    Noni“Wanwei No. 1”of noni that was introduced from the Big Island of Hawaii,USA and has been selected through series of selective breeding measures including seedling propagation and regional screening to select for superior growth,excellent fruition,strong resistant to diseases and wind,and well-adapted to new climate conditions. It has such bears the desired features of early maturity,high fruit yield with large fruits and high quality,and resilience to harsh growth condition; thus it is especially suitable for growing in tropical region of south and west of Hainan island and Yongxing Island,along the shoreline and coastal saline areas both as windbreaks and orchard. The fruits can be processed as healthy supplement.%‘万维1号’是从美国夏威夷大岛引种,采用选择育种方法经选株、选果、选种、选苗、实生苗繁殖测定和区域化试验选育而成,具有早产、高产、稳产、优质、抗递性强等特性。适于在海南岛的南部和西部、永兴岛等偏热带地区尤其是海岸线盐碱地区营建果园兼海防林,生产果实供食品加工。

  15. Progress in researches on the mechanisms of Morinda citrifolia L.%诺丽作用机制的研究进展

    Institute of Scientific and Technical Information of China (English)

    许国平; 张春妮

    2007-01-01

    在民间,诺丽早已作为药物被广泛应用,它对多种疾病有显著疗效.最近通过对诺丽的深入研究,鉴定出一些有效的药物成分,初步阐明了诺丽抗癌、抗氧化机制.现就诺丽作用机制的研究进展作一综述.

  16. 海巴戟干粉90 d喂养实验研究%EXPERIMENTAL STUDY ON NINETY DAYS FEEDING EXPERIMENT OF MORINDA CITRIFOLIA POWDER

    Institute of Scientific and Technical Information of China (English)

    冯丁山; 郑定仙; 林卫华; 黄业宇; 张晓昕

    2010-01-01

    [目的]为了判定海巴戟对人体长期服用的安全性,并为以后能将其开发成为功能性食品提供毒理学安全性的依据. [方法]将受试样品按1.7%、3.3%、8.O%的比例加入基础饲料中(分别相当于人群推荐日摄入量的50、100、240倍),采用经口给药方式进行90 d喂养试验检测. [结果]受试样品各剂量组对SD大鼠体重、进食量、食物利用率、血液学、血液生化、脏器重量以及脏体比等指标与正常对照组比较,均无统计学差异.未见与喂食样品有关的病理改变.大鼠的NOAEL为8.00 g/kg体重(相当于人体推荐摄入量的240倍). [结论]在实验研究剂量范围内此海巴戟干粉人体长期使用是安全可靠的.

  17. 海巴戟核心种质的构建方法%A Method for Establishing Core Collection of Morinda citrifolia

    Institute of Scientific and Technical Information of China (English)

    程江波; 邢诒旺; 赖茂良; 聂风琴; 林道哲; 孙仁毅; 符文英

    2014-01-01

    以126份海巴戟种质资源为材料,利用生物学性状、农艺学性状、SRAP分子标记等遗传多样性数据,采用逐步聚类法,开展海巴戟核心种质构建机制的研究.结果表明:海巴戟核心种质的最佳构建方法为Average系统聚类,G策略取样,15%的组内取样比例.应用该构建方法初步构建了由21个不同种质株系构成的海巴戟核心种质,该核心种质能够代表原种质的遗传多样性.

  18. Studies on anthraquinone biosynthesis in Morinda citrifolia suspension cells%海巴戟天悬浮细胞合成蒽醌类物质的研究

    Institute of Scientific and Technical Information of China (English)

    张志强; 李永成

    2014-01-01

    目的 研究外源激素、水杨酸、茉莉酸甲酯对海巴戟天悬浮细胞生长及蒽醌类化合物合成的影响.方法 建立海巴戟天细胞悬浮培养体系,并向海巴戟天细胞悬浮培养物中添加外源激素、水杨酸和茉莉酸甲酯,测定细胞比生长速率和蒽醌产量.结果 外源激素萘乙酸(NAA)、激动素(KT)对海巴戟天悬浮细胞的生长和蒽醌类化合物的合成有显著影响,采用B5培养基时,较佳激素组合为NAA2 mg/L+ KT 0.1 mg/L.在对数生长初期(培养9d)时,添加200 μmol/L水杨酸和50 μmol/L茉莉酸甲酯,蒽醌质量浓度分别为588.34 mg/L和595.49 mg/L,比对照分别提高了17.05%和18.47%.结论 适宜激素配比结合一定质量浓度的水杨酸和茉莉酸甲酯外源诱导对蒽醌类化合物的合成有促进作用.

  19. Studies on the Extraction of Total Flavonoids from Morinda citrifolia%海巴戟总黄酮提取工艺的研究

    Institute of Scientific and Technical Information of China (English)

    楚冬海; 许又凯; 高锦明; 纳智

    2008-01-01

    研究了海巴戟总黄酮最佳提取工艺.采用正交试验,以提取物中总黄酮的得率作为考察指标,优选出最佳提取工艺.试验设计的四因素中,提取时间对结果有极显著的影响,乙醇浓度对结果有显著的影响,提取温度和乙醇用量对结果影响不显著.海巴戟总黄酮的最佳提取工艺条件为:提取时间1.5 h,乙醇浓度90%,提取温度60~65℃,乙醇用量1:20(g/mL),按照最佳提取工艺条件提取海巴戟果实中的总黄酮,得率可达2.936%.

  20. Morinda citrifolia and Its Agronomic Characteristics in Xishuangbanna%诺丽在西双版纳的主要农艺性状表现

    Institute of Scientific and Technical Information of China (English)

    黄菁; 孙小龙; 刘昌芬; 蓝增全; 李海泉

    2010-01-01

    对引种的诺丽种质进行14个月的观测,结果表明4份种质在西双版纳均能正常开花结果.大田定植14个月的诺丽平均株高86.97cm,平均地径32.65mm,4个月的测产结果显示,平均单月结果数达11.74个,单果平均重量62.73g.

  1. Study on extracting method of pectin in Morinda Citrifolia fruit segiments%诺丽果渣中果胶的提取工艺研究

    Institute of Scientific and Technical Information of China (English)

    云韵琴

    2009-01-01

    分析研究了"万维1号"Noni(诺丽)果渣中的主要功能性成分,同时利用L9(34)正交实验法研究了Noni果渣中果胶的最佳提取工艺条件.结果表明:(1)"万维1号"Noni果渣中的主要功能性成分为多糖、总黄酮、果胶、黄酮甙和皂甙,其含量分别为249.8、87.80、43.80、9.9 mg/g和2.27 mg/g;(2)对"万维1号"Noni果渣中果胶的提取效果影响最大的因素是提取时间,其次是料液比.影响最小的是提取温度.果胶的最佳提取工艺条件为提取温度为90℃、提取时间为4.0 h、提取溶剂为0.05 mol/L HCI、料液比为1∶8,Noni果渣中果胶的含量为4.38%.

  2. Studies on jicama juice processing.

    Science.gov (United States)

    Juarez, M S; Paredes-Lopez, O

    1994-09-01

    Juice was extracted from jicama (Pachyrrizus erosus Urban) and clarified using a 10,000 daltons molecular weight cut-off membrane to improve its stability. Ultrafiltered juice was tested for general composition and Hunter color. Ultrafiltration (UF) retentate and UF permeate showed some changes, compared to fresh juice, in total and soluble solids, total sugars, and nitrogen, whereas ash and pH remained constant. Hunter color of juice samples exhibited some variation by UF. Results suggest that UF has potential to produce jicama juice with desirable and stable aroma and flavor.

  3. Natural Antioxidants: Piper sarmentosum (Kadok) and Morinda elliptica (Mengkudu).

    Science.gov (United States)

    Subramaniam, Vimala; Adenan, Mohd Ilham; Ahmad, Abdull Rashih; Sahdan, Rohana

    2003-03-01

    The antioxidant activity of two edible medicinal plants commonly used in Malaysian traditional medicine i.e. Piper sarmentosum (kadok) and Morinda elliptica (mengkudu) were tested for antioxidant activity. The methanolic leave extracts of kadok and mengkudu, at 250ug/ml, were tested using the Xanthine/Xanthine Oxidase (X/XOD) Superoxide Scavenging assay. Both extracts showed high superoxide scavenging assay, 88% and 80% respectively compared to superoxide dismutase (SOD) standard. The crude extracts were further fractionated using column chromatography and tested for superoxide scavenging activity, to obtain antioxidant active fractions. Two active fractions were obtained from kadok, PsFr6-71.3%, PsFr7-71.3%, and one active fraction from mengkudu, MeFr3-86.6%. These active fractions were compared against 14 phenolic compound standards. After a series of HPLC analysis of samples and standards, a natural antioxidant compound was identified in kadok and mengkudu i.e. Naringenin (4',5,7-Trihydroxyflavanone) with 75.7% superoxide scavenging activity. Naringenin is a highly potent natural antioxidant that has been reported in the raw materials of larch and grapefruit extracts. Thus, kadok and mengkudu which contain Naringenin, could be used as antioxidant dietary supplements.

  4. 21 CFR 156.145 - Tomato juice.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 2 2010-04-01 2010-04-01 false Tomato juice. 156.145 Section 156.145 Food and... CONSUMPTION VEGETABLE JUICES Requirements for Specific Standardized Vegetable Juices § 156.145 Tomato juice. (a) Identity—(1) Definition. Tomato juice is the food intended for direct consumption, obtained...

  5. 21 CFR 73.250 - Fruit juice.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 1 2010-04-01 2010-04-01 false Fruit juice. 73.250 Section 73.250 Food and Drugs... ADDITIVES EXEMPT FROM CERTIFICATION Foods § 73.250 Fruit juice. (a) Identity. (1) The color additive fruit juice is prepared either by expressing the juice from mature varieties of fresh, edible fruits, or by...

  6. 21 CFR 73.260 - Vegetable juice.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 1 2010-04-01 2010-04-01 false Vegetable juice. 73.260 Section 73.260 Food and... ADDITIVES EXEMPT FROM CERTIFICATION Foods § 73.260 Vegetable juice. (a) Identity. (1) The color additive vegetable juice is prepared either by expressing the juice from mature varieties of fresh, edible vegetables...

  7. Evaluation of noni (Morinda citrifolia volatile profile by dynamic headspace and gas chromatography-mass spectrometry Avaliação do perfil de voláteis em noni (Morinda citrifolia por headspace dinâmico e cromatografia gasosa-espectrometria de massas

    Directory of Open Access Journals (Sweden)

    Adriana Sousa

    2010-09-01

    Full Text Available Noni is a fruit that has interested the scientific community due to its medicinal and functional activities. Different products that contain noni are already in the market, but their consumption could be impaired by their distinctive unpleasant aroma and flavor. The aim of this work was to evaluate the noni pulp volatile profile by dynamic headspace and gas chromatography-mass spectrometry. Thirty seven volatile compounds were detected, mainly alcohols (63.3%, esters (26.9%, cetones (7.4%, and acids (1.2%.O noni é um fruto que tem interessado à comunidade científica por sua atividade funcional e medicinal. Já se encontram no mercado diferentes produtos que contêm noni em sua composição, mas seu consumo tem sido prejudicado por seu aroma e sabor desagradáveis. O objetivo deste trabalho foi o de avaliar o perfil de voláteis da polpa de noni pela técnica de headspace dinâmico e cromatografia gasosa-espectrometria de massas. Foram detectados 37 compostos voláteis, sendo os principais: alcoóis (63,3%, ésteres (26,9%, cetonas (7,4% e ácidos (1,2%.

  8. Inhibitory Effects of Morinda officinalis Extract on Bone Loss in Ovariectomized Rats

    Directory of Open Access Journals (Sweden)

    Qiao-Yan Zhang

    2009-06-01

    Full Text Available The present study was undertaken to investigate the protective effects of ethanol extract from the root of Morinda Officinalis (RMO on ovariectomy-induced bone loss. Administration of RMO extract increased trabecular bone mineral content and bone mineral density of tibia, improved the levels of phosphorus (P, calcium (Ca and OPG, decreased the levels of DPD/Cr, TRAP, ACTH and corticosterone, but did not reverse the levels of ALP, TNF-α and IL-6 in serum of ovariectomized rats. These findings demonstrated that RMO extract reduced bone loss in ovariectomized rats, probably via the inhibition of bone resorption, but was not involved with bone formation. Anthraquinones and polysaccharides from Morinda officinals could be responsible for their antiosteoporotic activity, and the action mechanism of these constituents needs to be further studied. Therefore, RMO has the potential to develop a clinically useful antiosteoporotic agent.

  9. Study on Oligosaccharides from Morinda officinalis%巴戟天寡糖研究

    Institute of Scientific and Technical Information of China (English)

    冯峰; 王羚郦; 赖小平; 李远彬; 曹志梅; 周应军

    2012-01-01

    Objective: To study the oligosaccharides from Morinda officinalis How. Methods: Compounds were isolated by chroma-tography, and their structures were identified by spectral analysis and chemical evidences. Results: six compounds were isolated and i-dentified as sucrose( I ) , inulin-type trisaecharide( II ) , inulin-type hexasaccharide( III) , inulotriose( IV) , inulotetraose( V ) , inu-lopentaose( VI).Conclusion: Compound IV ,V and VI are isolated from Morinda officinalis for the first time. Morinda officinalis How; Oligosaccharides; Inulin-type oligosaccharide; Inulo-type oligosaccharide%目的:对巴戟天(Morinda officinalis)的糖类成分进行化学研究.方法:用活性炭、纤维素和硅胶等色谱法分离,用波谱法对化合物结构进行鉴定.结果:从巴戟天活性部位中分离出6个寡糖类化合物,分别为蔗糖(Ⅰ)、耐斯糖(Ⅱ)、菊粉六糖(Ⅲ)、β-D-果吡喃糖-(2→1)-β-D-果呋喃糖-(2→1)-β-D-果呋喃糖(Ⅳ)、β-D-果吡喃糖-(2→1) -β-D-果呋喃糖-(2→1)-β-D-果呋喃糖-(2→1)-β-D-果呋喃糖(Ⅴ)、β-D-果吡喃糖-(2→1)-β-D-果呋喃糖-( 2→1)-β-D-果呋喃糖-(2→1)-β-D-果呋喃糖-(2→1)-β-D-果呋喃糖(Ⅵ).结论:化合物Ⅳ-Ⅵ为首次从巴戟天属中分离出的化合物.

  10. Hepatotoxicity and nephrotoxicity evaluation in Wistar albino rats exposed to Morinda lucida leaf extract

    Directory of Open Access Journals (Sweden)

    Taofeeq Oduola

    2010-05-01

    Full Text Available Background: Aqueous extract of Morinda lucida benth leaf is consumed in Southern Nigeria in the treatment of malaria without any regard for its safety. Aim: The aim of the study was to investigate the effects of ingestion of the ethanolic leaf extract of the plant on liver and kidney functions in Wistar albino rats. Materials and Methods: Acute oral toxicity test was performed to determine the LD50; sub-chronic toxicity study was then carried out by oral administration of different doses of the extract on daily basis to different groups of rats for 42 days. The animals were subsequently sacrificed, and liver and kidney functions assessed biochemically using standard techniques. Results: The acute oral toxicity result, LD50, revealed Morinda lucida leaf extract to be non-lethal at 6400mg/kg body weight. The results obtained for liver and kidney function parameters indicated that ingestion of Morinda lucida leaf extract has no toxic effect on liver and kidney functions. Conclusion: The results can form the basis for clinical trials in humans.

  11. Hepatotoxicity and nephrotoxicity evaluation in Wistar albino rats exposed to Morinda lucida leaf extract

    Directory of Open Access Journals (Sweden)

    Taofeeq Oduola

    2010-01-01

    Full Text Available Background: Aqueous extract of Morinda lucida benth leaf is consumed in Southern Nigeria in the treatment of malaria without any regard for its safety. Aim: The aim of the study was to investigate the effects of ingestion of the ethanolic leaf extract of the plant on liver and kidney functions in Wistar albino rats. Materials and Methods: Acute oral toxicity test was performed to determine the LD 50 ; sub-chronic toxicity study was then carried out by oral administration of different doses of the extract on daily basis to different groups of rats for 42 days. The animals were subsequently sacrificed, and liver and kidney functions assessed biochemically using standard techniques. Results: The acute oral toxicity result, LD 50, revealed Morinda lucida leaf extract to be non-lethal at 6400mg/kg body weight. The results obtained for liver and kidney function parameters indicated that ingestion of Morinda lucida leaf extract has no toxic effect on liver and kidney functions. Conclusion: The results can form the basis for clinical trials in humans.

  12. Composition of pomegranate juice.

    Science.gov (United States)

    Krueger, Dana A

    2012-01-01

    A database of 793 commercial pomegranate juices was analyzed to produce a profile for authentication of pure pomegranate juice. The database consisted of data from a mix of authentic and adulterated samples. Statistical tools were used to reduce the database to a stable sample set of 477 presumably authentic samples. The profile obtained (mean, SD at 16 Brix) are as follows: fructose (g/100 g) 6.83, 0.50; glucose (g/100 g) 6.66, 0.44; sucrose (g/100 g) 0.00, 0.00; sorbitol (g/100 g) 0.00, 0.01; acidity (g/100 g as citric acid) 1.25, 0.32; citric acid (g/100 g) 1.19, 0.30; malic acid (g/100 g) 0.065, 0.034; tartaric acid (g/100 g) 0.00, 0.00; isocitric acid (mg/kg) 63, 21; potassium (mg/kg) 2320, 400; proline (mg/kg) 7, 5; formol value [milliequivalents/100 g] 1.00, 0.24; 13C/12C ratio [o/oo Pee Dee belemnite]-26.4, 0.8. The profile samples had a consistent anthocyanin pattern consisting of four major peaks corresponding to delphinidin-3,5-diglucoside, delphinidin-3-glucoside, cyanidin-3,5-diglucoside, and cyanidin-3-glucoside. Minor peaks corresponding to pelargonidin-3,5-diglucoside and pelargonidin-3-glucoside were also generally present. No maltose, D-malic acid, or tartaric acid were detected in any of the samples. The profile obtained corresponds closely with previously published data.

  13. 21 CFR 146.137 - Frozen orange juice.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 2 2010-04-01 2010-04-01 false Frozen orange juice. 146.137 Section 146.137 Food... HUMAN CONSUMPTION CANNED FRUIT JUICES Requirements for Specific Standardized Canned Fruit Juices and Beverages § 146.137 Frozen orange juice. (a) Frozen orange juice is orange juice as defined in § 146.135...

  14. How Much Cranberry Juice Is in Cranberry-Apple Juice? A General Chemistry Spectrophotometric Experiment

    Science.gov (United States)

    Edionwe, Etinosa; Villarreal, John R.; Smith, K. Christopher

    2011-01-01

    A laboratory experiment that spectrophotometrically determines the percent of cranberry juice in cranberry-apple juice is described. The experiment involves recording an absorption spectrum of cranberry juice to determine the wavelength of maximum absorption, generating a calibration curve, and measuring the absorbance of cranberry-apple juice.…

  15. Questions and Answers: Apple Juice and Arsenic

    Science.gov (United States)

    ... Home Food Resources for You Consumers Questions & Answers: Apple Juice and Arsenic Share Tweet Linkedin Pin it ... MMA), may also be a health concern. Are apple and other fruit juices safe to drink? The ...

  16. Det globale marked for juice

    DEFF Research Database (Denmark)

    Hansen, Henning Otte

    2015-01-01

    Den internationale handel med juice har været i kraftig vækst, omend der siden finanskrisens start har været en mere jævn udvikling. Væksten kan især tilskrives Kina, som har haft en meget stor stigning i både produktion og eksport, og som i dag er centrum for international afsætning af juice. Især...... inden for æblejuice er Kina vigtig og står for 35 pct. af verdens samlede eksport. Den internationale handel med juice er meget omfattende, og mange lande er store både importører og eksportører. Den danske import af æblejuice kommer især fra Tyskland og Østrig, som dog også har en stor import fra Kina...

  17. Freeze concentration of lime juice

    Directory of Open Access Journals (Sweden)

    Ampawan Tansakul

    2008-11-01

    Full Text Available The main objective of this research was to study the effects of processing conditions, i.e. cooling medium temperature (-6, -12 and -18C and scraper blade rotational speed (50, 100 and 150 rpm on the freeze concentration of lime juice. The initial soluble solid content of lime juice was 7.6 Brix. Results showed that soluble solid content of lime juice increased as cooling medium temperature decreased while scraper blade rotational speed increased. It was also found that the processing condition with -18˚C cooling medium temperature and 150 rpm rotational speed of the scraper blade was the best among all studied conditions, although the loss of the soluble solids with ice crystals during ice separation was relatively high at 35%.

  18. Structure-activity studies: in vitro antileishmanial and antimalarial activities of anthraquinones from Morinda lucida

    DEFF Research Database (Denmark)

    Sittie, A A; Lemmich, E; Olsen, C E

    1999-01-01

    Anthraquinones have been isolated by bioassay-guided fractionation from Morinda lucida. Structure-activity studies show that an aldehyde group at C-2 and a phenolic hydroxy group at C-3 enhance the activity of the anthraquinones against the growth of Plasmodium falciparum and promastigotes of Lei...

  19. Meroterpenos (Antraquinonas en diferentes partes de la Planta de Morinda Royoc L

    Directory of Open Access Journals (Sweden)

    Janetsy Borroto Blanco

    2005-01-01

    Full Text Available Las antraquinonas son productos del metabolismo secundario con gran importancia en la industria farmaceútica. En el presente trabajo se determina la posible presencia de estos compuestos en diferentes partes de la Morinda royoc L. El análisis cualitativo (Reacción de Bortranger mostró una fuerte coloración roja en las raíces (médula y corteza indicativo de la presencia de antraquinonas y en mucha menor intensidad en las hojas (coloración rosada muy tenue. En el análisis cuantitativo, las raíces (médula y corteza mostraron los mayores valores, seguidos por las hojas, y prácticamente nula la presencia en el resto de los órganos estudiados. El procedimiento de purificación utilizado para el estudio más detallado de las raíces mostró la presencia de antraquinonas en las fracciones más polares (3-6. De todos los resultados obtenidos quedó demostrada la presencia de compuestos isoprenoides relacionados con las antraquinonas en las raíces de la Morinda royoc L. Llegar a establecer su composición y estructura requiere de estudios más especializados como HPLC-UV/DAP, RMN y Espectroscopia de Masa.

  20. Antimicrobial Activity and Phytochemical Analysis of Morinda tinctoria Roxb. Leaf Extracts

    Institute of Scientific and Technical Information of China (English)

    K Deepti; PUmadevi; GVijayalakshmi; BVinod polarao

    2012-01-01

    Objective: The objective of the present work is to evaluate the presence of Phytochemical constituents and antimicrobial activity of different extracts of leaves of Morinda tinctoria Roxb. Methods: The serial exhaustive extraction was done with a series of solvents: Hexane, Chloroform, Ethylacetate and Methanol with increasing polarity using soxhlet apparatus. The Phytochemical analysis was done by using the standard procedures. Antimicrobial activity was evaluated by Agar well diffusion method against nine human pathogens. Results: The results revealed that the leaf extracts contain a broad spectrum of secondary metabolites: Alkaloids, Phytosterols, Flavonoids, Phenols and Triterpenes in major proportion. Methanol extract was shown to be more effective against all the organisms followed by Ethylacetate, Chloroform and Hexane extracts. Proteus vulgaris (24mm) was found to be most sensitive organism followed by Klebsiella pneumonia (21mm) and Enterococcus feacelis (21mm). Conclusions: The present study concludes that the different extracts of M. tinctoria leaves contain a broad spectrum of secondary metabolites and also exhibit antimicrobial activity against all the tested microorganisms. It can also be concluded that Morinda tinctoria plant can be exploited to discover the bioactive natural products that may serve as leads in the development of new pharmaceuticals.

  1. Advertising and Product Confusion: A Case Study of Grapefruit Juice

    OpenAIRE

    Mark G. Brown; Lee, Jonq-Ying; Behr, Robert M.

    1990-01-01

    Demand relationships for two closely related products -- grapefruit juice and grapefruit-juice cocktail -- were estimated from grocery-store scanner data to analyze the contention that consumer confusion exists between the two products. Results suggest confusion may exist, with grapefruit-juice advertising not only increasing the demand for grapefruit juice but also for grapefruit-juice cocktail.

  2. 21 CFR 146.187 - Canned prune juice.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 2 2010-04-01 2010-04-01 false Canned prune juice. 146.187 Section 146.187 Food... HUMAN CONSUMPTION CANNED FRUIT JUICES Requirements for Specific Standardized Canned Fruit Juices and Beverages § 146.187 Canned prune juice. (a) Canned prune juice is the food prepared from a water extract of...

  3. 21 CFR 146.145 - Orange juice from concentrate.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 2 2010-04-01 2010-04-01 false Orange juice from concentrate. 146.145 Section 146... Juices and Beverages § 146.145 Orange juice from concentrate. (a) Orange juice from concentrate is the food prepared by mixing water with frozen concentrated orange juice as defined in § 146.146 or...

  4. 21 CFR 146.151 - Orange juice for manufacturing.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 2 2010-04-01 2010-04-01 false Orange juice for manufacturing. 146.151 Section... Fruit Juices and Beverages § 146.151 Orange juice for manufacturing. (a) Orange juice for manufacturing is the food prepared for further manufacturing use. It is prepared from unfermented juice obtained...

  5. Change of juice color during raspberry processing in fruit juice and fruit juice concentrate

    Directory of Open Access Journals (Sweden)

    Vukosavljević Predrag

    2006-01-01

    Full Text Available A change of anthocyanins under different conditions of enzymatic treatments, clarification and concentration was determined. A pectin preparation Klerzyme®120, manufactured by DSM, France, specific for "sour fruits" with pH below 3.2, was used for maceration and depectinization. Experiments were carried out by a laboratory hydraulic press. Raw raspberry juice was clarified either by membranes separation processes or by traditional treatments using gelatin and bentonite. For microfiltration and ultrafiltration processes, membrane cut-off should not be below 30,000 g/mol to prevent any color loss. Experiments with membrane separations processes were carried out with five different membranes. A raw depectinized raspberry juice was clarified by cross-flow microfiltration and ultrafiltration using ceramic tubular membranes and hollow fiber polymeric membranes of a molecular weight cut-off of 300, 50 and 30 kg /mol or with a mean pore size of 0.2 urn in the case of microfiltration. Fruit juice concentrations were carried out by a laboratory equipment for vacuum evaporation. Extraction yield by a laboratory hydraulic press was the same in case of single-stage maceration and two-stage maceration. However, due to a lower viscosity, it was observed that single-stage process provides raspberry juice with more color and high efficiency of extraction. It was noticed that thermal breaks of raspberry pulp provide juice containing more total anthocyanins. The clarification using gelatin and bentonite removed about 50% total anthocyanins, while a clarification by a cross-flow ultrafiltration using Carbosep M9, M8 and M7 membranes achieved the highest level of color loss. Total color loss after concentration was 70%. The best results in color protection were observed by a microfiltration through Kerasep membrane, due to its relatively large pores (0.2μm. .

  6. Antioxidant activity of potato juice

    Directory of Open Access Journals (Sweden)

    Przemysław Kowalczewski

    2012-06-01

    Full Text Available Background. The interest in potato juice as a therapeutic agent goes back to the 19th century but its application was not supported by any knowledge about biological activity of this raw material. Factors restricting the medical application of potato juice include its inattractive sensory and functional properties. The aim of the presented investigations was preliminary evaluation of the biological activity of potato juice and the impact on it of some technological operations such as: cryoconcentration and hydrolysis in a membrane reactor. Material and methods. Experiments comprised investigations of antioxidative potentials of fresh potato juice, products of its processing as well as fractions separated because of the size of their molecules using, for this purpose, Folin-Ciocalteu methods and reactions with the ABTS cation radical. Results. The value of the antioxidative potential of fresh potato juice determined by means of the ABTS reagent corresponded to approximately 330 μmol/100 g which is in keeping with literature data. As a result of the cryoconcentration process, the value determined by the Folin-Ciocalteu method was found to increase only slightly whereas the value determined by means of the ABTS reagent almost tripled. The antioxidative potential was found to grow even more strongly in the case of the application of both methods when the process of enzymatic hydrolysis was employed. The total of 5 protein fractions of molecular masses ranging from 11 000 Da to over 600 000 Da, as well as an organic non-protein fraction of the molecular mass of 600 Da, were obtained as a result of the performed separation. All the examined fractions exhibited antioxidative activities. The highest values determined by the Folin-Ciocalteu method were recorded for the protein fraction of 80 000 Da mean molecular mass, while using the ABTS reagent – for the organic, non-protein fraction. Conclusions. Potato juice possesses antioxidative activity which

  7. Grapefruit Juice and Some Drugs Don't Mix

    Science.gov (United States)

    ... For Consumers Home For Consumers Consumer Updates Grapefruit Juice and Some Drugs Don't Mix Share Tweet ... a similar way to grapefruit juice. How Grapefruit Juice Can Interfere With Medications With most drugs that ...

  8. 27 CFR 24.237 - Spirits added to juice or concentrated fruit juice.

    Science.gov (United States)

    2010-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2010-04-01 2010-04-01 false Spirits added to juice or concentrated fruit juice. 24.237 Section 24.237 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE BUREAU, DEPARTMENT OF THE TREASURY LIQUORS WINE Spirits § 24.237 Spirits added to juice or...

  9. Effect of Morinda Tinctoria Leaves Extract on the Corrosion Inhibition of Mild Steel in Acid Medium

    Institute of Scientific and Technical Information of China (English)

    K.Krishnaveni; J.Ravichandran; A.Selvaraj

    2013-01-01

    The Morinda tinctoria (MT) plant leaves extract was prepared in aqueous and hydrochloric acid media and was used as corrosion inhibitor for mild steel in hydrochloric acid medium.MT is found to be an efficient inhibitor at room temperature and the efficiency decreases with increase in temperature.Results from colorimetric studies predict the amount of iron present in the test solution and the percentage inhibition efficiency values calculated from this data fit well with the weight loss experiments.The AC impedance studies reveal that the mild steel surface is positively charged and the process of inhibition is through charge transfer.Polarisation studies indicate the mixed nature of the inhibitor.Thermodynamic parameters obtained predict that the process of inhibition is a spontaneous one.

  10. Multi-elements determination in medical and edible Alpinia oxyphylla and Morinda officinalis and their decoctions by ICP-MS.

    Science.gov (United States)

    Zhao, Xiangsheng; Wei, Jianhe; Shu, Xiaoyan; Kong, Weijun; Yang, Meihua

    2016-12-01

    Contents of twenty elements (Mg, K, Ca, Na, Fe, Al, Zn, Ba, Mn, Cu, Mo, Cr, Ni, As, Se, Cd, Hg, Tl, Pb and V) in two medical and edible plant species, Alpinia oxyphylla and Morinda officinalis were simultaneously determined by inductively coupled plasma-mass spectrometry (ICP-MS) method after microwave digestion with HNO3-H2O2 (6:1, v/v) as the digestion solvent. Certified standard reference material Poplar leaf was used to assess the accuracy of the method. The greatest contents of Mg, K, Ca, Al, Fe and Na were found in dried Alpinia oxyphylla and Morinda officinalis samples. The contents of five heavy metals including Pb, Cd, As, Hg and Cu in Alpinia oxyphylla did not exceed the limits. The contents of Pb in 76.67% samples and Cd in two batches of Morinda officinalis samples exceeded the limits set by Chinese Pharmacopeia. The contents of the selected elements in different parts (leaves, stems, roots and fruits) of Alpinia oxyphylla varied considerably. The highest concentrations of Mg, Ca, Mn and Se were found in the leaves of Alpinia oxyphylla, at the same time, while, the contents of 9 elements including Cd, Cr, Cu, As, Pb in the roots were the highest. The transfer ratios of selected elements from both species of herbs into their decoctions were reduced. Especially for the heavy metals, the transfer ratios were below 30% except As (79.73%) in Morinda officinalis. The results showed that decoction of the samples may reduce the intake of heavy metals. Copyright © 2016 Elsevier Ltd. All rights reserved.

  11. [Effect of Morinda lucida Benth. (Rubiaceae) and Newbouldia leavis P. Beauv. (Bignoniaceae) on sickling of red blood cells].

    Science.gov (United States)

    Joppa, K M; Vovor, A; Eklu-Gadegbeku, K; Agbonon, A; Aklikokou, K; Gbeassor, M

    2008-06-01

    The purpose of this study was to evaluate the In vitro anti-sickling activity of two plants widely used for treatment of sickle cell disease in Togo, i.e., Morinda lucida et Newbouldia leavis. A concentration-dependent decrease in the rate of sickling was observed after incubation of red blood cells with plant extracts and 2% sodium metabisulfite as compared to incubation with 0.9% NaCl. On samples with a SS blood genotype the inhibition rate of Morinda lucida was 17.30% at a concentration of 1 mg/ml and 92.31% at a concentration of 30 mg/ml. On samples with an AS blood genotype, the inhibition rate of Morinda lucida 48.10% at a concentration of 1 mg/ml and 99.34% at a concentration of 30 mg/ml. Using Newbouldia leavis the inhibition rates at concentrations of 1 mg/ml and 30 mg/ml were 15.66% and 90.42% respectively on samples with a SS blood genotype and 64.03% and 99.02% respectively on samples with an AS blood genotype. The study protocol appeared to be adequate for both SS and AS blood genotypes since the Pearson correlation coefficient between rates measured on the two types of samples was 0.92 for Newuboulida and 0.89 for Morinda. These findings show that these two plants have clear-cut in vitro anti-sickling activity and support their use in traditional medicine.

  12. Modes of Inhibition of α-Amylase and α-Glucosidase by Aqueous Extract of Morinda lucida Benth Leaf

    Directory of Open Access Journals (Sweden)

    M. I. Kazeem

    2013-01-01

    Full Text Available Diabetes mellitus is a metabolic disorder of glucose metabolism. The management of blood glucose level is the hallmark in the treatment of this disease. This may be achieved through the use of oral hypoglycemic drugs such as biguanides, insulin secretagogues, and α-glucosidase inhibitors. The purpose of the present study was to investigate the inhibitory effect of Morinda lucida leaf extracts on the activities of α-amylase and α-glucosidase. This was performed using α-amylase from Aspergillus oryzae and α-glucosidase from Saccharomyces cerevisiae. Aqueous extract of Morinda lucida gave the highest percentage yield (9.99% of the plant out of the three extracts (compared to acetone and ethanolic extracts and possesses the highest inhibitory activity against α-amylase (IC50 value of 2.30 mg/mL and α-glucosidase (IC50 value of 2.00 mg/mL. Kinetic analysis revealed that the aqueous extract of this plant leaf inhibited the α-amylase competitively but displayed mixed noncompetitive mode of inhibition towards α-glucosidase. It can be concluded that aqueous extract of Morinda lucida exhibited the best inhibitory activity on the two enzymes studied and the presence of phytochemicals like flavonoids, saponins, and tannins may have contributed greatly to the inhibitory activity of the plant extract.

  13. Clementine juice has the potential for drug interactions - In vitro comparison with grapefruit and mandarin juice.

    Science.gov (United States)

    Theile, Dirk; Hohmann, Nicolas; Kiemel, Dominik; Gattuso, Giuseppe; Barreca, Davide; Mikus, Gerd; Haefeli, Walter Emil; Schwenger, Vedat; Weiss, Johanna

    2017-01-15

    Adverse drug interactions due to grapefruit juice are well known prompting warnings even in drug labels. Similar issues have not been reported for clementines and available data is scarce, despite of genetic descent. We observed substantially increased tacrolimus trough concentrations in a renal transplant patient consuming high clementine amounts and, thus, scrutinised the effects of clementine juice on drug metabolism and drug transporters in vitro and compared it to the effects of mandarin and grapefruit juice. All citrus juices profoundly induced several drug transporters and drug metabolising enzymes, whereas the effects of grapefruit juice were most pronounced (e.g. 156-fold and 34-fold induction of cytochrome P450 (CYP) 3A4 mRNA by grapefruit juice and clementine juice, respectively). However, the juices also inhibited e.g. CYP3A4, raising the question which effect prevails in vivo. Using an enzymatic activity assay, we demonstrated that at least in vitro CYP3A4 inhibition prevails for both grapefruit and clementine juice, whereas for CYP1A2 induction appears to predominate. Thus, inhibition of CYP3A4 is presumably the underlying reason for the observed increase in the concentrations of the CYP3A4 substrate tacrolimus in the patient. Taken together, our data indicate that clementine juice as well as grapefruit juice and to a lesser extent also mandarin juice can induce several important drug metabolising enzymes and drug transporters, but also inhibit some of these proteins. Our data indicate that clementine juice similar to grapefruit juice bears the potential for profound interactions with drugs potentially leading to adverse drug effects e.g. through over-exposure to CYP3A4 substrates. Copyright © 2016 Elsevier B.V. All rights reserved.

  14. 76 FR 5822 - Orange Juice From Brazil

    Science.gov (United States)

    2011-02-02

    ... to consider include technology; production methods; development efforts; ability to increase... COMMISSION Orange Juice From Brazil AGENCY: United States International Trade Commission. ACTION: Institution of a five-year review concerning the antidumping duty order on certain orange juice from Brazil...

  15. Spoilage of fruit juices by filamentous fungi

    Science.gov (United States)

    Contamination of molds in fruit juices has risen in recent years. Even though there are many critical control points in the processing protocols that are noted and maintained, there remains a problem with dairy and juices packed in paperboard cartons. This talk discusses the work involved in the dis...

  16. Vitamin C Content of Commercial Orange Juices

    Science.gov (United States)

    Haddad, Paul

    1977-01-01

    Describes an experiment designed to confirm that newly purchased commercial orange juice contains sufficient ascorbic acid to meet government standards, and to establish the rate of aerial oxidation of this ascorbic acid when the juice is stored in a refrigerator. (MLH)

  17. Microbiological Quality of Fresh Nopal Juice.

    Science.gov (United States)

    Hernández-Anguiano, Ana María; Landa-Salgado, Patricia; Eslava-Campos, Carlos Alberto; Vargas-Hernández, Mateo; Patel, Jitendra

    2016-12-10

    The consumption of fresh nopal cactus juice is widely popular among health-conscious consumers in Mexico. The juice is prepared from fresh cladodes that have only been rinsed with tap water and are not subjected to a pasteurization or terminal bacterial reduction process. The aim of this study was to evaluate the microbial quality of commercially available fresh juices (n = 162) made with nopal in Texcoco, State of Mexico, during the summer and spring season. Standard microbiological methods, the PCR technique and the serological method were used for isolation and identification of bacteria. All samples contained total coliforms and 91% were positive for Escherichia coli. Although total coliforms and E. coli were detected throughout the study, their populations were significantly lower (p nopal juices is unacceptable due to its health significance. The information generated in this study is relevant for human health risk assessment associated with the consumption of unpasteurized nopal juices and potential interventions to minimize pathogen contamination.

  18. Effect of Processing on the Quality of Pineapple Juice

    NARCIS (Netherlands)

    Hounhouigan, M.H.; Linnemann, A.R.; Soumanou, M.M.; Boekel, van M.A.J.S.

    2014-01-01

    Pineapple processing plays an important role in juice preservation. Because the quality of the pineapple juice is affected by the processing technology applied, the effects of pasteurization and other preservation methods on the overall juice quality were discussed. During juice processing,

  19. 21 CFR 146.146 - Frozen concentrated orange juice.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 2 2010-04-01 2010-04-01 false Frozen concentrated orange juice. 146.146 Section... (CONTINUED) FOOD FOR HUMAN CONSUMPTION CANNED FRUIT JUICES Requirements for Specific Standardized Canned Fruit Juices and Beverages § 146.146 Frozen concentrated orange juice. (a) Frozen concentrated orange...

  20. Effect of Processing on the Quality of Pineapple Juice

    NARCIS (Netherlands)

    Hounhouigan, M.H.; Linnemann, A.R.; Soumanou, M.M.; Boekel, van M.A.J.S.

    2014-01-01

    Pineapple processing plays an important role in juice preservation. Because the quality of the pineapple juice is affected by the processing technology applied, the effects of pasteurization and other preservation methods on the overall juice quality were discussed. During juice processing, microorg

  1. 21 CFR 146.153 - Concentrated orange juice for manufacturing.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 2 2010-04-01 2010-04-01 false Concentrated orange juice for manufacturing. 146... Canned Fruit Juices and Beverages § 146.153 Concentrated orange juice for manufacturing. (a) Concentrated orange juice for manufacturing is the food that complies with the requirements of composition and label...

  2. Selección de semillas y capacidad de germinación de Ficus citrifolia Mill.

    OpenAIRE

    2014-01-01

    Se analizó la selección por método gravitacional y la capacidad de germinación de semillas del higuerón (Ficus citrifolia Mill), especie identificada con la protección de vertientes en los bosques secos de la provincia de Loja. Se evidenció la germinación de semilla sedimentada con 34,5% de promedio con un máximo poder de germinación a los 15 días.

  3. Production and Preservation of Passion Fruit Juice

    Directory of Open Access Journals (Sweden)

    U. G. AKPAN

    2005-06-01

    Full Text Available Production and preservation of Passion Fruit Juice was examined to reduce the spoilage and to increase the shelf life of the juice using chemical preservatives. The preservation of the juice was carried out using sugar, benzoic acid, citric and a combination of citric and benzoic acid under room temperature.The result revealed that the juice maintained its color, aroma and tastes for at least one month when 30% benzoic acid was used as preservative. This happens to be the best among all. The juice under other preservation like 4% sugar went bad after three days, while that of 4% citric acid maintained its qualities for one week and some days, but thereafter the aroma started to fade. The combination of 3% benzoic acid and 4% citric acid maintained the qualities of the juice fairly between two to three weeks. The alcoholic content was also estimated and it was discovered that the juice containing citric acid and sugar has the highest percentage of alcohol. The preservation used also altered the pH so that it is impossible for pathogens to exist at such a low pH environment.

  4. Photosynthetic Daily Changes Characteristics of Four Different Morinda citrifolia Linn. Species in Xishuangbanna%西双版纳引种的四个诺丽品系光合日变化研究

    Institute of Scientific and Technical Information of China (English)

    李法营; 高进波; 蓝增全; 刘昌芬; 李青红

    2010-01-01

    应用Li-Cor6400便携式光合测定仪对生长在西双版纳的4个不同诺丽品系光合作用日变化进行测定.结果表明:‘小黑’和‘美国’品系的Pn呈单峰,而‘印尼’和‘海南’品系的呈现双峰,有明显的“午休”现象;‘印尼’品系的Gs日变化趋势与Pn大致一致,表明Pn的变化主要受气孔因素作用的结果;‘海南’品系的Gs日变化趋势与Pn不太一致;表明Pn的变化主要受气孔和非气孔因素共同作用的结果;PARi和Tr是对光合特性影响最大的因子.

  5. Determination of Trace Elements in Morinda Citrifolia by Flame Atomic Absorption Spectrometry%火焰原子吸收光谱法测定海巴戟中的微量元素

    Institute of Scientific and Technical Information of China (English)

    赵俊凌

    2013-01-01

    利用火焰原子吸收光谱法研究海巴戟中微量元素的含量.将海巴戟用微波消解后,采用火焰原子吸收光谱法分别测定海巴戟中8种微量元素.海巴戟中K,Ca,Na,Mg,Zn,Fe,Mn及Cu的含量分别为695.12,223.15,871.06,363.08,581.55,225.32,86.77,13.00μg/g,回收率在94.35%-97.96%之间.海巴戟含有丰富的人体必需微量元素.

  6. 不同栽培基质对海巴戟幼苗生长的影响%Effects of Different Cultivation Substrates on the Growth of Morinda citrifolia Seedlings

    Institute of Scientific and Technical Information of China (English)

    谭运洪; 张艳军; 张远辉

    2009-01-01

    [目的]筛选比较适合海巴戟幼苗生长的基质配方.[方法]试验采用随机区组设计,以生土为对照,设6个处理:①园土+草炭土+农家肥、②椰糠+草炭土+农家肥、③椰糠+园土+农家肥、④椰糠+珍珠岩+草炭土+农家肥、⑤椰糠+珍珠岩+园土+草炭土+农家肥、⑥园土,研究6种不同栽培基质对海巴戟幼苗生长的影响.[结果]处理④的植株生长最好,与处理⑥和对照差异极显著.处理③、④的平均株高分别为22.90和21.64 cm;处理④、⑤的平均茎基分别为0.69和0.66 cm;处理③、④的平均叶片数分别为24.53和24.13;处理④的单株地上、地下部分鲜重分别达86.3和25.9 g.[结论]椰糠+珍珠岩+草炭土+农家肥(1∶1∶1∶1)为海巴戟幼苗生长的最佳配方.

  7. 海巴戟总黄酮提取工艺的响应面法优化%Optimization of Extraction Conditions of Flavonoids from Morinda citrifolia by Response Surface Methodology

    Institute of Scientific and Technical Information of China (English)

    赵俊凌

    2013-01-01

    目的 采用响应面法对海巴戟总黄酮的提取工艺进行优化.方法 在单因素实验的基础上,以乙醇浓度、提取温度、提取时间及料液比为自变量,总黄酮含量为响应值,研究各因素及其交互作用对总黄酮得率的影响.结果 最优工艺条件为,乙醇浓度95%,液料比为1∶30 g.ml-1,提取温度为87.4℃,浸提时间为120 min,提取实际值为13.991 mg·g-1.结论 采用响应面法对海巴戟黄酮提取条件进行优化合理可行.

  8. Determination of 18 amino acids in Morinda citrifolia L.by pre-column derivation RP-HPLC%柱前衍生法测定海巴戟中18种氨基酸

    Institute of Scientific and Technical Information of China (English)

    李戈; 赵俊凌

    2015-01-01

    目的:建立柱前衍生高效液相色谱法测定海巴戟中18种氨基酸的方法.方法:以2,4-二硝基氟苯为柱前衍生剂,梯度洗脱,流速1.0mL/min,检测波长为360nm,柱温为30℃.结果:18种氨基酸的线性范围分别为32-609μg/mL,r值均0.9990-0.9998,平均回收率分别为91.87%-108.56%.结论:本方法可用于海巴戟中氨基酸的测定.

  9. Study on the flower structure and flowering rule of Morinda citrifolia (Noni)%海巴戟(Noni)的花器结构和花期规律的研究

    Institute of Scientific and Technical Information of China (English)

    符洁; 何书奋; 蔡开朗

    2015-01-01

    采用田间和室内相结合,固定植株跟踪观察法,观察研究海巴戟(Noni)花器结构和花期规律.结果表明:不同品种间其花器结构无明显差异;在海南,海巴戟周年开花,单花的开花周期为35h,初开、盛开和谢花三个过程,每年的7月份为盛花期;单花序从初花至尾花开放持续时间平均45d,平均开花数20朵,授粉方式为自花授粉.论文还就花器的结构特例,花器结构对授粉方式的影响和海巴戟的果实形态是否为聚合果进行了讨论.

  10. 响应面法优化诺丽多糖提取工艺%Optimization of Extraction Conditions of Polysaccharide from Morinda citrifolia by Response Surface Methodology

    Institute of Scientific and Technical Information of China (English)

    李戈; 赵俊凌

    2015-01-01

    目 的采用响应面法对诺丽多糖的提取工艺进行优化.方法 在单因素实验的基础上,以提取温度、液料比、提取时间为自变量,总多糖含量为响应值,研究各因素及其交互作用对总多糖得率的影响.结果 最优工艺条件为,液料比为1∶40g· mL-1,提取温度为100C,浸提时间为4h,提取实际值为9.677mg·g-1.结论 采用响应面法优化诺丽总多糖提取工艺是合理可行的,实验结果将为诺丽多糖新产品研发提供物质基础.

  11. Investigation on Diversity of Endophytic Bacterial Community in Xisha Wild Noni (Morinda citrifolia L.) Seed%西沙野生诺尼种子内生细菌群落多样性的初步研究

    Institute of Scientific and Technical Information of China (English)

    刘洋; 李辉; 李金霞; 曹艳花; 姚粟; 白飞荣; 谭望桥; 程池

    2013-01-01

    通过构建16S rDNA克隆文库的非培养方法对我国西沙野生诺尼种子内生细菌群落结构多样性进行研究,共涉及Proteobacteria、Firmicutes、Bacteroidetes及Actinobacteria四大类群,包含33个属,42个OTU,优势菌属(丰度)依次为Enterobacter sp.(17.55%)、Bacillus sp.(16.79%)、Acinetobacter sp.(6.11%)、Pseudomonas sp.(6.11%)及Piscinibacter sp.(6.11%).这是国内外首次利用非培养方法针对诺尼种子内生细菌群落多样性进行研究的报道.

  12. Determination of polysaccharide in fruits of morinda citrifolia and analysi s of its component%海巴戟果水提物中多糖含量的测定及组成分析

    Institute of Scientific and Technical Information of China (English)

    刘海青; 张娜; 徐剑锋; 徐桂云

    2008-01-01

    采用苯酚-硫酸法测定了海巴戟果水提物中多糖的含量.测定波长490 nm,多糖换算因子f=1.26;在8~56 mg/L 范围内,其浓度与吸收度线性关系良好,R2=0.9984;多糖的平均回收率为100.15 %,RSD为0.8%(n=9);测定海巴戟果水提物中多糖的含量为11.7 7 %.气相色谱法分析结果表明,海巴戟果多糖是由岩藻糖、木糖、甘露糖、半乳糖、果糖等单糖组成.

  13. 海巴戟天愈伤组织诱导及细胞悬浮培养试验%Study on callus induction and cell suspension culture of Morinda citrifolia

    Institute of Scientific and Technical Information of China (English)

    张志强; 李永成

    2014-01-01

    以海巴戟天叶脉作为外植体,在B5培养基上诱导出淡黄色、疏松愈伤组织,通过增殖后接种到液体培养基中,建立细胞悬浮培养体系,并对影响细胞悬浮培养的培养基种类、接种量、pH值、蔗糖含量等因素进行了研究.结果表明:B5+ 1mg/L2,4-D+ 0.1 mg/L 6-BA能诱导出淡黄色、质地疏松的愈伤组织;较佳愈伤组织增殖培养基为B5+2 mg/L NAA+ 0.2 mg/L KT;细胞在B5+2 mg/L NAA+ 0.2 mg/L KT培养基中悬浮培养,接种量60 g/L(细胞鲜重)、蔗糖4%、pH值5.8、100 r/min、27℃下暗培养,海巴戟天悬浮细胞比生长速率为0.0836 d-1,总蒽醌含量367.8 mg/L,培养周期27~30 d.

  14. Distribution and Nutritional Evaluation of Primary Functional Components in Morinda citrifolia%诺丽果实和叶中主要功能性物质的分布与营养评价

    Institute of Scientific and Technical Information of China (English)

    张伟敏; 符文英; 施瑞城; 张海德

    2008-01-01

    为了综合开发诺丽系列食品,分析研究诺丽果和叶中主要营养与功能性物质的分布与营养价值.结果表明:叶子中的蛋白质、脂肪和水分的含量要明显高于果实(p<0.05),而还原糖和VC的含量则明显低于果实(p<0.05).且叶中必需氨基酸的含量要明显高于果实(p<0.05),诺丽果实和叶中均含有丰富的微量元素.而叶中的微量元素要明显高于诺丽果实.在同一诺丽品种中,其不同部位中的主要营养成分与功能性成分有所不同;且诺丽果和叶中所含有这些成分和物质,表明诺丽果和叶具有较好的开发潜力和价值.

  15. Isolation and identification of endophytic bacteria from the fruit of Morinda citrifolia(Noni)%海巴戟天(诺尼)果内生细菌的初步分离与鉴定

    Institute of Scientific and Technical Information of China (English)

    程池; 曹艳花; 姚粟; 李金霞; 李辉; 刘洋

    2014-01-01

    首次对采自我国海南三亚市诺尼植物果实内生细菌进行初步分离与鉴定研究,相关研究为诺尼果内生菌研究奠定基础.采用传统微生物学分离培养方法对28℃培养条件下分离得到的15株细菌进行16S rDNA扩增和系统发育分析.研究表明,分离得到的15株诺尼果内生细菌分属于变形菌门(Proteobacteria)、厚壁菌门(Firmicutes)和放线菌门(Actinobacteria)3个类群,包括甲基杆菌属(Methylobacterium sp.)、鞘氨醇单胞菌属(Sphingomonas sp.)、芽孢杆菌属(Bacillus sp.)、节杆菌属(Arthrobacter sp.)、棒杆菌属(Corynebacterium sp.)、考克氏菌属(Kocuria sp.)和链霉菌属(Streptomyces sp.)7个属的11个已知种.研究结果表明,诺尼果中内生细菌具有较为丰富的多样性,获得了一批应用前景广泛的诺尼果内生细菌资源.

  16. 干燥条件对海巴戟天果中水分和蒽醌类化合物含量的影响%The Effects of Drying Conditions on Moisture Transfer and Anthraquinones Contents in Morinda Citrifolia

    Institute of Scientific and Technical Information of China (English)

    2016-01-01

    在真空干燥、密闭干燥、热风干燥条件下,研究干燥温度为50 ℃、60 ℃、70 ℃和样品厚度(1 mm、2 mm、3 mm)对海巴戟天果的干燥动力学和蒽醌类化合物含量的影响.水分的干燥曲线表明干燥动力学和有效水分扩散率的关系,阿伦尼乌斯方程则表示扩散系数与温度的关系.干燥条件、样品厚度(1 mm、2 mm和3 mm厚度)以及干燥温度影响干燥率和蒽醌类化合物含量.真空干燥条件具有更高的干燥效率,水分扩散率和葸醌类化合物含量均较高.当样品厚度为3 mm,真空条件下扩散系数8.82×10-10 m2/S,活化能为36.52 K J/mol.

  17. The Analysis on Correlation between Yield and Growth Vigor Factors of Morinda Citrifolia%诺丽果结果量与生长势因素相关性分折

    Institute of Scientific and Technical Information of China (English)

    杨丽薇

    2010-01-01

    文章以海南省林业科学研究所云龙种苗基地15月生诺丽果林6个样方共36株为观测对象,分别以树高、地-径、平均冠幅、分枝数为生长势指标,探讨各项指标与诺丽果结果量的相关性,通过总体相关性方差分析结果表明:诺丽果树高、地径和冠幅大小对结果量的影响达到极显著水平,分枝数对结果量的影响达到显著水平.在此基础上,采用进入和逐步回归分析法进行分析,建立树高、地径、平均冠幅、分枝数预测诺丽果结果量方程模型为:y=-72.584-0.015x1+25.127x2+0.352x3-1.004x4或y=-72.561+24.854x2+0.346x3-1.001x4,对预测诺丽果结果量具有科学参考价值,可为评价诺丽果种植水平及诺丽果产品开发和市场拓展提供科学决策依据.

  18. Observation and study on Morinda citrifolia blossom habit and pollination methods%海巴戟开花习性与授粉方式的观察和研究

    Institute of Scientific and Technical Information of China (English)

    何书奋; 符洁; 林道哲; 邢诒旺; 符文英

    2013-01-01

    采用了田间和室内相结合,固定植株跟踪观察的方法,对海巴戟(Noni)开花习性与授粉方式进行了观察研究.结果表明:海巴戟在海南周年开花,开花的数量和质量一年四季有所不同,开花盛期为每年的4-7月.每朵花从开放到凋谢的时阃平均2d,开花高峰期在每天清晨9-10h,下午也有开花,授粉后花辩掉落.单花序从初花至尾花开放持续时间平均42d,平均开花数20朵,海巴戟授粉方式为自花授粉.文中就双受精对海巴戟果实膨大的影响,花器结构对授粉方式的影响等展开了讨论.

  19. Communities Diversity of Endophytic Bacteria from Fruit of Morinda citrifolia (Noni)%西沙野生诺尼内生细菌群落多样性初步研究

    Institute of Scientific and Technical Information of China (English)

    曹艳花; 刘洋; 姚粟; 李金霞; 谭望桥; 程池

    2014-01-01

    采用构建16S rDNA克隆文库方法,首次对我国海南永兴岛西沙野生诺尼果内生细菌的群落结构和多样性进行初步研究.首次采用Mothur软件对诺尼果内生细菌克隆文库数据进行分析,构建Mothur软件数据分析平台.构建的西沙野生诺尼果内生细菌16S rDNA克隆文库中,93个克隆分属于12个不同可操作分类单元(operational taxonomic units,OTU),序列比对结果表明大部分克隆与已知细菌16S rDNA序列相似性较高.分别属于变形菌门(Proteobacteria)的Alpha、Beta、Gamma亚群,放线菌门(Actinobacteria)和厚壁菌门(Firmicutes).水库杆菌属(Piscinibacter sp.)细菌为野生诺尼果内生细菌优势菌群.优势菌属分别为水库杆菌属(Piscinibacter sp.)为80.65%,蛋白胨菌属(Peptoniphilus sp.)和链球菌属(Streptococcus sp.)均为3.23%,甲基杆菌属(Methylobacterium sp.)为2.15%.研究结果表明西沙野生诺尼果中存在较为丰富的内生细菌资源,将为进一步研究奠定理论基础.

  20. 诺丽(NONI)果与叶中总黄酮的提取工艺%Study on extracting method of flavonoid from fruit and leaf of morinda citrifolia

    Institute of Scientific and Technical Information of China (English)

    云韵琴; 符文英; 符传贤

    2009-01-01

    利用L9 (34) 正交试验法研究了Noni果与叶中总黄酮的提取工艺条件及其含量测定.结果表明:对Noni果中总黄酮的提取效果影响最大的因素是提取时间,其次是乙醇浓度和提取温度,影响最小的是料液比.总黄酮的最佳提取工艺条件为提取温度为75℃,提取时间为4.0h,提取溶剂为75%的乙醇,料液比为1 ∶ 30,Noni果粗提物中总黄酮的含量为22.91mg/g.对Noni果叶中总黄酮的提取效果影响最大的因素是提取温度,其次是料液比和乙醇浓度,影响最小的是提取时间.总黄酮的最佳提取工艺条件为提取温度为85℃,提取时间为4.0h,提取溶剂为80%的乙醇,料液比为1 ∶ 35,其总黄酮的得率为33.04mg/g.

  1. Investigation on diversity of entophytic fungus community in Xisha wild Noni (Morinda citrifolia L.) seed%西沙野生诺尼种子内生真菌的群落多样性

    Institute of Scientific and Technical Information of China (English)

    程池; 李辉; 刘洋; 李金霞; 姚粟; 白飞荣; 谭望桥

    2013-01-01

    采用种子表面灭菌、菌种分离与纯化、ITS rDNA和26S rDNA D1/D2区序列测定和系统发育分析方法,对西沙野生诺尼种子内生真菌群落多样性进行了初步研究.从诺尼种子中共分离到内生真菌48株,其中酵母菌39株,霉菌9株;39株酵母菌分属于4个属内的5个种,其中榛针孢酵母(Eremothecium coryli)、Pseudozyma aphidis和浅黄隐球酵母(Cryptococcus flavescens)为优势种,分别占总菌株数的31.25%、27.08%和16.67%;9株霉菌分属于4个属内的4个种,其中Phaeoacremonium sp.和赤霉属(Gibberella sp.)是霉菌中相对优势的种,分别占总菌株数的8.33%和6.25%.研究结果表明,西沙野生诺尼种子中存在大量内生真菌,且种类丰富,其中酵母菌占绝对优势,部分优势种具有潜在的药用价值.

  2. Changes of Total Flavonoids Content in Vegetative Organs of Morinda citrifolia (Noni) in Different Growth Periods%诺丽营养器官内的总黄酮含量及消长规律

    Institute of Scientific and Technical Information of China (English)

    孟蕲翾; 崔孟媛; 吴友根; 杨东梅; 张军锋

    2014-01-01

    利用紫外分光光度法对诺丽(Noni)营养器官及枝叶不同生长阶段中总黄酮含量的消长规律进行研究,以探索诺丽营养器官内总黄酮的动态积累,为生产中确定适宜的采收期及其采收部位提供依据.结果表明:(1)该方法测定诺丽中总黄酮含量重复性好,稳定性强,加标回收率达99.66%,测试结果稳定可靠;(2)诺丽各器官总黄酮的含量顺序为:90 d叶>270 d叶>30 d叶>毛状根>210 d叶>150 d叶>侧根>330 d叶>主根>嫩枝>老枝>2年生枝>多年生枝>1年生枝.结果表明,对诺丽中总黄酮的开发利用应重点选择叶片,采摘生长270 d的叶入药,有利于植株生长,并可提高诺丽资源的综合利用率.

  3. Separation and Screening of Endophytic Bacteria in Xisha Wild Noni (Morinda citrifolia L.) Seed%西沙野生诺尼种子内生细菌的分离与筛选

    Institute of Scientific and Technical Information of China (English)

    程池; 刘洋; 曹艳花; 李辉; 李金霞; 姚粟; 谭望桥

    2013-01-01

    通过微生物培养方法首次对我国西沙野生诺尼种子内生细菌进行分离及筛选,共得到20株内生细菌,经形态学观察、16S rDNA扩增及系统发育分析,将其归为变形菌门(Proteobacteria)中的肠杆菌(Enterobacter sp.)、伯克霍尔德氏菌(Burkholderia sp.)和泛菌(Pantoea sp.)3个菌属.

  4. GC-MS Analysis of the Ethyl Ether Soaking Extraction from Morinda citrifolia Seeds%诺丽籽乙醚冷浸提取物的GC-MS分析

    Institute of Scientific and Technical Information of China (English)

    张伟敏; 却志群; 云韵琴; 施瑞城

    2009-01-01

    为分析诺丽(Noni)籽乙醚冷浸提取物中的成分,采用乙醚冷浸提取法提取诺丽籽中的成分,应用气相色谱-质谱联用法对化学成分进行鉴定,用峰面积归一化法测定各个化合物在乙醚冷浸提取物中的相对百分含量.鉴定出45个峰中的35个化合物,占提取物总含量的96.52%.主要化合物为酸和酯类化合物,其中酸类化合物占42.89%;酯类化合物占45.92%;苯衍生物占2.69%,另外,还含有少量的烷烃占0.56%等.

  5. Estimating bergamot juice adulteration of lemon juice by high-performance liquid chromatography (HPLC) analysis of flavanone glycosides.

    Science.gov (United States)

    Cautela, Domenico; Laratta, Bruna; Santelli, Francesca; Trifirò, Antonio; Servillo, Luigi; Castaldo, Domenico

    2008-07-09

    The chemical composition of 30 samples of juices obtained from bergamot (Citrus bergamia Risso and Poit.) fruits is reported and compared to the genuineness parameters adopted by Association of the Industry of Juice and Nectars (AIJN) for lemon juice. It was found that the compositional differences between the two juices are distinguishable, although with difficulty. However, these differences are not strong enough to detect the fraudulent addition of bergamot juice to lemon juice. Instead, we found the high-performance liquid chromatography (HPLC) analysis of the flavanones naringin, neohesperidin, and neoeriocitrin, which are present in bergamot juice and practically absent in the lemon juice, is a convenient way to detect and quantify the fraudulent addition of bergamot juice. The method has been validated by calculating the detection and quantification limits according to Eurachem procedures. Employing neoeriocitrin (detection limit = 0.7 mg/L) and naringin (detection limit = 1 mg/L) as markers, it is possible to detect the addition of bergamot juice to lemon juice at the 1% level. When using neohesperidin as a marker (detection limit = 1 mg/L), the minimal percentage of detectable addition of bergamot juice was about 2%. Finally, it is reported that the pattern of flavonoid content of the bergamot juice is similar to those of chinotto (Citrus myrtifolia Raf) and bitter orange (Citrus aurantium L.) juices and that it is possible to distinguish the three kinds of juices by HPLC analysis.

  6. Grape Juice: Same Heart Benefits as Wine?

    Science.gov (United States)

    ... juice offer the same heart benefits as red wine? Answers from Katherine Zeratsky, R.D., L.D. ... some of the same heart benefits of red wine, including: Reducing the risk of blood clots Reducing ...

  7. for a cottage cashew juice factory.

    African Journals Online (AJOL)

    facilities under the small-scale enterprise scheme to adopt this technology. Introduction ... export earnings in Nigeria which increased to N743 ... areas and further increase in world .... Cost, Returns and Probability of the juice Extraction Factory.

  8. Spray Drying of Mosambi Juice in Lab

    Science.gov (United States)

    Singh, S. V.; Verma, A.

    2014-01-01

    The studies on spray drying of mosambi juice were carried out with Laboratory spray dryer set-up (LSD-48 MINI SPRAY DRYER-JISL). Inlet and outlet air temperature and maltodextrin (drying agent) concentration was taken as variable parameters. Experiments were conducted by using 110 °C to 140 °C inlet air temperature, 60 °C to 70 °C outlet air temperature and 5-7 % maltodextrin concentration. The free flow powder of mosambi juice was obtained with 7 % maltodextrin at 140 °C inlet air temperature and 60 °C outlet air temperature. Fresh and reconstituted juices were evaluated for vitamin C, titrable acidity and sensory characteristics. The reconstituted juice was found slightly acceptable by taste panel.

  9. FUNCTIONAL BEVERAGES BASED ON VEGETABLE JUICES

    Directory of Open Access Journals (Sweden)

    Limareva N. S.

    2014-01-01

    Full Text Available This article covers development of functional beverages technology based on using vegetable juice with apple and beetroot pectin concentrates, content of vitamins, minerals and functional properties

  10. The Orange Juice Distribution Channel: Some Characteristics ...

    African Journals Online (AJOL)

    PROMOTING ACCESS TO AFRICAN RESEARCH ... in retailing and also in the FCOJ (Frozen Concentrated Orange Juice) supply. These changes are threatening and also bringing opportunities to companies. This paper has two objectives.

  11. Shaji Juice, Healthy Drink from Desert

    Institute of Scientific and Technical Information of China (English)

    Bai Yifeng; Sun Yongjian

    2007-01-01

    @@ We are going to promote the Shaji juice to the world. At present, it has been sold to overseas markets including Japan and Taiwan. The Shaji products, such as Shaji juice and Shaji milk, are organic beverages which have not only very nice tastes, but also be very good to health.Therefore, I am very confident that it will be accepted by the international market.Standard Chartered Bank Outlines China

  12. The effective use of acai juice, blueberry juice and pineapple juice as negative contrast agents for magnetic resonance cholangiopancreatography in children

    Energy Technology Data Exchange (ETDEWEB)

    Bittman, Mark E. [Cohen Children' s Medical Center of New York, North Shore Long Island Jewish Health System, Department of Radiology, New Hyde Park, NY (United States); Callahan, Michael J. [Boston Children' s Hospital, Department of Radiology, Boston, MA (United States)

    2014-07-15

    Magnetic resonance cholangiopancreatography (MRCP) is commonly performed in the evaluation of known or suspected pancreaticobiliary disease in children. The administration of a negative oral contrast agent can improve the quality of the examination without significant additional cost. We describe our experience with certain brands of acai juice, blueberry juice and pineapple juice as negative oral contrast agents in children. We believe these fruit juices are safe, palatable and may improve MRCP image quality. (orig.)

  13. Morphological and mineral analysis of dental enamel after erosive challenge in gastric juice and orange juice.

    Science.gov (United States)

    Braga, Sheila Regina Maia; De Faria, Dalva Lúcia Araújo; De Oliveira, Elisabeth; Sobral, Maria Angela Pita

    2011-12-01

    This study evaluated and compared in vitro the morphology and mineral composition of dental enamel after erosive challenge in gastric juice and orange juice. Human enamel specimens were submitted to erosive challenge using gastric juice (from endoscopy exam) (n = 10), and orange juice (commercially-available) (n = 10), as follows: 5 min in 3 mL of demineralization solution, rinse with distilled water, and store in artificial saliva for 3 h. This cycle was repeated four times a day for 14 days. Calcium (Ca) loss after acid exposure was determined by atomic emission spectroscopy. The presence of carbonate (CO) and phosphate (PO) in the specimens was evaluated before and after the erosive challenge by FT-Raman spectroscopy. Data were tested using t-tests (P enamel was observed in scanning electron microscopy (SEM). The mean loss of Ca was: 12.74 ± 3.33 mg/L Ca (gastric juice) and 7.07 ± 1.44 mg/L Ca (orange juice). The analysis by atomic emission spectroscopy showed statistically significant difference between erosive potential of juices (P = 0.0003). FT-Raman spectroscopy found no statistically significant difference in the ratio CO/PO after the erosive challenge. The CO/PO ratios values before and after the challenge were: 0.16/0.17 (gastric juice) (P = 0.37) and 0.18/0.14 (orange juice) (P = 0.16). Qualitative analysis by SEM showed intense alterations of enamel surface. The gastric juice caused more changes in morphology and mineral composition of dental enamel than orange juice. The atomic emission spectroscopy showed to be more suitable to analyze small mineral loss after erosive challenge than FT-Raman. Copyright © 2011 Wiley Periodicals, Inc.

  14. Research on the Influencing Factors of China Apple Juice Trade

    Directory of Open Access Journals (Sweden)

    Du Juan

    2013-06-01

    Full Text Available China is the first country in the world in which apple juice is produced and exited and the main producing area is concentrated in the north of China. Some domestic companies which export apple juice are founded. China’s apple juice, mainly exported to USA, Japan and the Europe, has a strong international competitiveness. However, due to the breed and raw material, Chinese apple juice export faces some challenge, like the loss happening in the transport process. The objective of this study is to research China's apple juice export situation and problem using the comparative analysis method. To cut down the loss, this study is trying to offer a relative scientific research for fruit juice industry by analyzing how temperature and concentration influence on thermal conductivity of apple juice, affecting the whole juice industry. It is with great significance to solve the realistic problems and promote China apple juice industry and its international trade.

  15. Phytochemical screening and chemical variability in volatile oils of aerial parts of Morinda morindoides.

    Science.gov (United States)

    Kiazolu, J Boima; Intisar, Azeem; Zhang, Lingyi; Wang, Yun; Zhang, Runsheng; Wu, Zhongping; Zhang, Weibing

    2016-10-01

    Morinda morindoides is an important Liberian traditional medicine for the treatment of malaria, fever, worms etc. The plant was subjected to integrated approaches including phytochemical screening and gas chromatography mass spectrometry (GC-MS) analyses. Phytochemical investigation of the powdered plant revealed the presence of phenolics, tannins, flavonoids, saponins, terpenes, steroidal compounds and volatile oil. Steam distillation followed by GC-MS resulted in the identification of 47 volatiles in its aerial parts: 28 were in common including various bioactive volatiles. Major constituents of leaves were phytol (43.63%), palmitic acid (8.55%) and geranyl linalool (6.95%) and stem were palmitic acid (14.95%), eicosane (9.67%) and phytol (9.31%), and hence, a significant difference in the percentage composition of aerial parts was observed. To study seasonal changes, similarity analysis was carried out by calculating correlation coefficient (r) and vector angle cosine (z) that were more than 0.91 for stem-to-stem and leaf-to-leaf batches indicating considerable consistency.

  16. In vitro multiplication of Morinda royoc L. in Temporary Immersion Systems

    Directory of Open Access Journals (Sweden)

    Elio Jiménez

    2011-04-01

    Full Text Available Morinda royoc L. is a medicinal plant which has identified numerous secondary metabolites important for medical and pharmaceutical industry. The use of in vitro culture techniques could contribute to the production of these. The aim of this work was to multiply M. royoc using Temporary Immersion System (TIS. It was used TIS of 1000 ml capacity. Each contained 250 ml MS medium with 4.4 μM benzyladenine (BA and 2.9 μM indole acetic acid (IAA. The SIT were inoculated with 30 individual explants (shoot tips and nodal segments. It was determined the effect of the immersion frequency (two, four and six immersions of two minutes per day and the type of explants (shoot tips, nodal segments on the multiplication of shoots and biomass production. It was found that with four and six immersions per day the highest values of multiplication coefficient and shoot length were obtained. The maximum biomass production was achieved with six immersions per day. No hyperhidricity symptoms were observed in shoots. It was noted that the nodal segments produced more shoots per explant, increased multiplication coefficient and biomass than the apex, while the latter resulted in longer shoots. Keywords: micropropagation, shoots multiplication

  17. [Study on intestinal absorption features of oligosaccharides in Morinda officinalis How. with sigle-pass perfusion].

    Science.gov (United States)

    Deng, Shao-Dong; Zhang, Peng; Lin, Li; Xiao, Feng-Xia; Lin, Jing-Ran

    2015-01-01

    To study the in situ intestinal absorption of five oligosaccharides contained in Morinda officinalis How. (sucrose, kestose, nystose, 1F-Fructofuranosyinystose and Bajijiasu). The absorption of the five oligosaccharides in small intestine (duodenum, jejunum and ileum) and colon of rats and their contents were investigated by using in situ single-pass perfusion model and HPLC-ELSD. The effects of drug concentration, pH in perfusate and P-glycoprotein inhibitor on the intestinal absorption were investigated to define the intestinal absorption mechanism of the five oligosaccharides in rats. According to the results, all of the five oligosaccharides were absorbed in the whole intestine, and their absorption rates were affected by the pH of the perfusion solution, drug concentration and intestinal segments. Verapamil Hydrochloride could significantly increase the absorptive amount of sucrose and Bajijiasu, suggesting sucrose and Bajijiasu are P-gp's substrate. The five oligosaccharides are absorbed mainly through passive diffusion in the intestinal segments, without saturated absorption. They are absorbed well in all intestines and mainly in duodenum and jejunum.

  18. Rapid bioassay for the study of growth promoting activity of Morinda pubescens leaf extract

    Institute of Scientific and Technical Information of China (English)

    Desai Nivas; Gaikwad DK

    2013-01-01

    Objective: To study the influence of aqueous extracts of Morinda pubescens Smith. (M. pubescens) on the germination (%) and seedling growth (fresh and dry weight) of wheat and fenugreek. Methods:The various concentrations (0.05%, 0.1%, 0.15%, 0.25%, 0.50%, 1%, 1.25% and 2.5%) of these AE were prepared and used for the germination trials. Distilled water was used as control. Results:Aqueous extracts at the concentration of 0.15% and 0.25% shows significant stimulatory effect on seed germination and seedling growth of wheat and fenugreek which also found significant in the significant enhancement in root and shoot legth, vigour index and mobilization efficiency of wheat as compared to control, while fenugreek seeds exhibits stimulatory response at these concentrations. It was also noticed that seed germination and seedling growth is sensitive to higher concentrations of leaf extract showing its inhibitory allelopathic effect. Conclusion: These findings indicate that aqueous leaf extract of M. pubescens possess biotonic potential.

  19. Potential interaction between pomegranate juice and warfarin.

    Science.gov (United States)

    Komperda, Kathy E

    2009-08-01

    To my knowledge, no published reports have described an interaction between pomegranate juice and warfarin. Investigators from previous animal and in vitro studies have reported a potential for pomegranate juice to inhibit metabolism involving the cytochrome P450 system, an effect that could translate into a clinical drug-diet interaction with warfarin. This case report describes a 64-year-old Caucasian woman who was treated with warfarin for recurrent deep vein thrombosis. She had been receiving a relatively stable dosage of warfarin 4 mg/day for several months, with stable international normalized ratios (INRs). During that time, the patient was consuming pomegranate juice 2-3 times/week. She stopped drinking the juice, and her INRs became subtherapeutic. Her dosage of warfarin was increased to maintain therapeutic anticoagulation. No rechallenge with pomegranate juice was performed. Use of the Drug Interaction Probability Scale indicated a possible relationship between the patient's subtherapeutic INR and the pomegranate juice. Although this potential interaction needs to be explored further, clinicians should be aware of the interaction and thoroughly interview and closely monitor their patients who are receiving warfarin.

  20. [Grapefruit juice and drugs: a hazardous combination?].

    Science.gov (United States)

    Lohezic-Le Devehat, F; Marigny, K; Doucet, M; Javaudin, L

    2002-01-01

    A single glass of grapefruit juice can improve the oral bioavailability of a drug thus either increasing its efficacy or enhancing its adverse effects particularly if the therapeutic index is narrow. Grapefruit juice acts by inhibiting presystemic drug metabolism mediated by CYP P450 3A4 in the small bowel and this interaction would appear to be more relevant if the CYP 3A4 content is high and the drug has a strong first pass degradation. Intestinal P-glycoprotein may also be affected by grapefruit juice. The compounds responsible for this food-drug interaction have not as yet been identified but this phenomenon could result from a complex synergy between flavonoids (naringin, naringenin), furanocoumarins (6',7'-dihydroxybergamottin, bergamottin) and sesquiterpen (nootkatone). In our study, we report the mechanisms of action of grapefruit juice and the interactions between grapefruit juice and 42 drugs; to date, only 12 drugs showed no interaction. Taking these results into consideration, patients should be educated about grapefruit juice intake with medication.

  1. Juicing the Juice: A Laboratory-Based Case Study for an Instrumental Analytical Chemistry Course

    Science.gov (United States)

    Schaber, Peter M.; Dinan, Frank J.; St. Phillips, Michael; Larson, Renee; Pines, Harvey A.; Larkin, Judith E.

    2011-01-01

    A young, inexperienced Food and Drug Administration (FDA) chemist is asked to distinguish between authentic fresh orange juice and suspected reconstituted orange juice falsely labeled as fresh. In an advanced instrumental analytical chemistry application of this case, inductively coupled plasma (ICP) spectroscopy is used to distinguish between the…

  2. Juicing the Juice: A Laboratory-Based Case Study for an Instrumental Analytical Chemistry Course

    Science.gov (United States)

    Schaber, Peter M.; Dinan, Frank J.; St. Phillips, Michael; Larson, Renee; Pines, Harvey A.; Larkin, Judith E.

    2011-01-01

    A young, inexperienced Food and Drug Administration (FDA) chemist is asked to distinguish between authentic fresh orange juice and suspected reconstituted orange juice falsely labeled as fresh. In an advanced instrumental analytical chemistry application of this case, inductively coupled plasma (ICP) spectroscopy is used to distinguish between the…

  3. ORANGE JUICE AND BLOOD PRESSURE

    Directory of Open Access Journals (Sweden)

    M. F. VALIM

    2009-01-01

    Full Text Available

    Blood pressure is the force of blood against artery walls. It is measured in millimeters of mercury (mm Hg and recorded as two numbers: systolic pressure (as the heart contracts over diastolic pressure (as the heart relaxes between beats. High blood pressure (hypertension is defined as chronically elevated high blood pressure, with systolic blood pressure (SBP of 140 mm Hg or greater, and diastolic blood pressure (DBP of 90 mm Hg or greater. High blood pressure (HBP, smoking, abnormal blood lipid levels, obesity and diabetes are risk factors for coronary heart disease, the leading cause of death in the US. Lifestyle modifications such as engaging in regular physical activity, quitting smoking and eating a healthy diet (limiting intake of saturated fat and sodium and increasing consumption of fiber, fruits and vegetables are advocated for the prevention, treatment, and control of HBP. As multiple factors influence blood pressure, the effects of each factor are typically modest, particularly in normotensive subjects, yet the combined effects can be substantial. Nutrition plays an important role in influencing blood pressure. Orange juice should be included as part of any low sodium diet and/or any blood pressure reducing eating plan, as it is sodium free, fat-free and can help meet recommended levels of potassium intake that may contribute to lower BP.

  4. Beetroot juice and exercise performance

    Directory of Open Access Journals (Sweden)

    Ormsbee MJ

    2013-11-01

    Full Text Available Michael J Ormsbee,1 Jon Lox,1 Paul J Arciero2 1Department of Nutrition, Food, and Exercise Sciences, Human Performance Lab, Florida State University, Tallahassee, FL, USA; 2Department of Health and Exercise Sciences, Human Nutrition and Metabolism Lab, Skidmore College, Saratoga Springs, NY, USA Abstract: Increased sales and consumption of organic and natural foods reflect consumers heightened interest in promoting health and improving athletic performance. Of these products, beetroot and its constituents have become increasingly popular in the arena of exercise performance, mainly due to the high concentrations of nitrate. Studies have indicated beetroot juice (BRJ may improve exercise time to exhaustion, running performance, and increase muscular efficiency during moderate intensity exercise. The purpose of this review is to examine the efficacy of BRJ to serve as an ergogenic aid in athletic performance. It appears that BRJ may provide modest performance enhancement; however, more research is needed to clearly identify mechanisms of action and proper dosing patterns to maximize the performance benefits of BRJ. Keywords: beetroot, nitrate, betaine, sports nutrition

  5. Amelioration of oxidative stress in bio-membranes and macromolecules by non-toxic dye from Morinda tinctoria (Roxb.) roots.

    Science.gov (United States)

    Bhakta, Dipita; Siva, Ramamoorthy

    2012-06-01

    Plant dyes have been in use for coloring and varied purposes since prehistoric times. A red dye found in the roots of plants belonging to genus Morinda is a well recognized coloring ingredient. The dye fraction obtained from the methanolic extract of the roots of Morinda tinctoria was explored for its role in attenuating damages caused by H(2)O(2)-induced oxidative stress. The antioxidant potential of the dye fraction was assessed through DPPH radical scavenging, deoxyribose degradation and inhibition of lipid peroxidation in mice liver. It was subsequently screened for its efficiency in extenuating damage incurred to biomembrane (using erythrocytes and their ghost membranes) and macromolecules (pBR322 DNA, lipids and proteins) from exposure to hydrogen peroxide. In addition, the non-toxic nature of the dye was supported by the histological evaluation conducted on the tissue sections from the major organs of Swiss Albino mice as well as effect on Hep3B cell line (human hepatic carcinoma). The LC-MS confirms the dye fraction to be morindone. Our study strongly suggests that morindone present in the root extracts of M. tinctoria, in addition to being a colorant, definitely holds promise in the pharmaceutical industry.

  6. Cytoprotective effects of Morinda officinalis against hydrogen peroxide-induced oxidative stress in Leydig TM3 cells

    Institute of Scientific and Technical Information of China (English)

    Mun-Seog Chang; Won-Nam Kim; Woong-Mo Yang; Hyu-Young Kim; Ji-Hoon Oh; Seong-Kyu Park

    2008-01-01

    Aim: To investigate the antioxidant effects of Morinda officinalis (Morindae radix, MR) on H202-induced oxidative stress in cultured mouse TM3 Leydig cells. Methods: We carded out 2,2-diphenyl-l-picrylhydrazyl free radical scavenging, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, lipid peroxidation, testosterone enzyme immunoassay, superoxide dismutase (SOD), and catalase (CAT) assays in Leydig TM3 cells. Results: MR showed a 47.8% 2,2-diphenyl-1-picrylhydrazyl radical scavenging effect in TM3 cells with no significant cytotoxicity. Oxi- dative stress was induced in TM3 cells with 100 μmol H2O2, and treatment of the cells with 250 μg/mL MR showed the most significant protective effect (64%, P < 0.001) in the cell viability assay with a decreased lipid peroxidation level (1.75 nmol/mg protein, P < 0.05), increased testosterone production (43.5 pg/mL), and improvements in SOD activity (7.49 units of SOD/mg protein, P < 0.001) and CAT activity (74.6 units of CAT/mg protein, P < 0.001). Conclusion: These findings indicate that MR, as an antioxidant, protects functions of cultured mouse TM3 Leydig cells from H2O2-induced oxidative stress. (Asian J Androl 2008 Jul; 10: 667-674)

  7. No Fruit Juice Before Age 1, Pediatricians Say

    Science.gov (United States)

    ... medlineplus.gov/news/fullstory_165826.html No Fruit Juice Before Age 1, Pediatricians Say Plus, they support ... toddlers into tantrums. One recommendation is that fruit juice be limited for toddlers and older children, and ...

  8. Fruit Juice for Kids: A Serving a Day OK

    Science.gov (United States)

    ... page: https://medlineplus.gov/news/fullstory_164260.html Fruit Juice for Kids: A Serving a Day OK Review ... 2017 (HealthDay News) -- Pediatricians have long suggested that fruit juice may prompt weight gain in children, but a ...

  9. apple juice's clarification process by using cassava and rice starch

    African Journals Online (AJOL)

    SARAH

    2015-11-30

    Nov 30, 2015 ... vegetable juices includes steps like extraction, clarification, and stabilization .... given by INRAB. Methods. Juice extraction: The collected cashew fruits were ...... Clarification, Preservation and Shelf Life. Evaluation of Cashew ...

  10. 21 CFR 146.150 - Canned concentrated orange juice.

    Science.gov (United States)

    2010-04-01

    ... of orange juice soluble solids in degrees Brix; for example, a 62° Brix concentrate in 1-gallon cans may be named on the label “canned concentrated orange juice, 62° Brix”. If the food does not...

  11. Inhibition of foodborne pathogens by pomegranate juice.

    Science.gov (United States)

    Haghayeghi, Koorosh; Shetty, Kalidas; Labbé, Ronald

    2013-05-01

    Pomegranates have health-promoting benefits because of their polyphenol constituents. Previous studies have demonstrated the antimicrobial activity of aqueous and organic extracts of pomegranate components and by-products. We sought to determine the antimicrobial activity against 40 foodborne pathogens representing eight bacterial species using juice itself. In addition, we sought to determine the synergistic antimicrobial activity between pomegranate juice and other plant products displaying antimicrobial activity. The antimicrobial activity of pomegranate juice was dependent on the test organism, which varied to highly susceptible (four Gram-positive species) to unaffected (Salmonella and Escherichia coli O157:H7). Two Gram-negative species, which were inhibited were Helicobacter pylori and Vibrio parahemolyticus. No synergistic antimicrobial activity was seen between pomegranate and either barberry, oregano, or cranberry. The antimicrobial activity of pomegranate juice is dependent on the test organism and extraction method. The sensitivity of H. pylori suggests that pomegranate juice may be an alternative or supplemental treatment for gastric ulcers caused by this organism.

  12. Microbiological Quality of Fresh Nopal Juice

    Directory of Open Access Journals (Sweden)

    Ana María Hernández-Anguiano

    2016-12-01

    Full Text Available The consumption of fresh nopal cactus juice is widely popular among health-conscious consumers in Mexico. The juice is prepared from fresh cladodes that have only been rinsed with tap water and are not subjected to a pasteurization or terminal bacterial reduction process. The aim of this study was to evaluate the microbial quality of commercially available fresh juices (n = 162 made with nopal in Texcoco, State of Mexico, during the summer and spring season. Standard microbiological methods, the PCR technique and the serological method were used for isolation and identification of bacteria. All samples contained total coliforms and 91% were positive for Escherichia coli. Although total coliforms and E. coli were detected throughout the study, their populations were significantly lower (p < 0.05 in winter and spring, respectively. Citrobacter youngae was found in 20% of the samples, an unidentified species of Citrobacter in 10%, C. freundii and Proteus mirabilis in 3%, and Salmonella Javiana in 1%. The presence of these microorganisms, especially Salmonella, in the nopal juices is unacceptable due to its health significance. The information generated in this study is relevant for human health risk assessment associated with the consumption of unpasteurized nopal juices and potential interventions to minimize pathogen contamination.

  13. Microbiological Quality of Fresh Nopal Juice

    Science.gov (United States)

    Hernández-Anguiano, Ana María; Landa-Salgado, Patricia; Eslava-Campos, Carlos Alberto; Vargas-Hernández, Mateo; Patel, Jitendra

    2016-01-01

    The consumption of fresh nopal cactus juice is widely popular among health-conscious consumers in Mexico. The juice is prepared from fresh cladodes that have only been rinsed with tap water and are not subjected to a pasteurization or terminal bacterial reduction process. The aim of this study was to evaluate the microbial quality of commercially available fresh juices (n = 162) made with nopal in Texcoco, State of Mexico, during the summer and spring season. Standard microbiological methods, the PCR technique and the serological method were used for isolation and identification of bacteria. All samples contained total coliforms and 91% were positive for Escherichia coli. Although total coliforms and E. coli were detected throughout the study, their populations were significantly lower (p < 0.05) in winter and spring, respectively. Citrobacter youngae was found in 20% of the samples, an unidentified species of Citrobacter in 10%, C. freundii and Proteus mirabilis in 3%, and Salmonella Javiana in 1%. The presence of these microorganisms, especially Salmonella, in the nopal juices is unacceptable due to its health significance. The information generated in this study is relevant for human health risk assessment associated with the consumption of unpasteurized nopal juices and potential interventions to minimize pathogen contamination. PMID:27973398

  14. Utilization of dried roselle calyces extract in fruit juice processing

    OpenAIRE

    Kilima, Beatrice Mgaya

    2014-01-01

    There has been an increased consumption of fruit juice as consumers have become aware of the nutritional and health benefits of fruit juices. This increased consumption goes together with the growth of varieties of fruit juices and beverages offered for sale. Among these products is roselle (Hibiscus sabdariffa L.) juice, or drink, which is a good source of anthocyanins, vitamins (thiamine, riboflavin, niacin, ascorbic acid) and minerals (Ca, P, Fe). In addition, roselle is also used as flavo...

  15. Insecticidal activity and chemical composition of the Morinda lucida essential oil against pulse beetle Callosobruchus maculatus.

    Science.gov (United States)

    Owolabi, Moses S; Padilla-Camberos, Eduardo; Ogundajo, Akintayo L; Ogunwande, Isiaka A; Flamini, Guido; Yusuff, Olaniyi K; Allen, Kirk; Flores-Fernandez, Karen Isabel; Flores-Fernandez, Jose Miguel

    2014-01-01

    Insecticidal activity of essential oil extracted from Morinda lucida was tested on pulse beetle Callosobruchus maculatus, which is a pest that causes serious damage to several pulses. The insecticidal activity was compared with two pesticides, Phostoxin and Primo-ban-20. 120 mixed sex adult C. maculatus were introduced, along with 30 g of cowpeas. Four concentrations (0.40, 0.20, 0.10, and 0.05 μg/mL) of the M. lucida essential oil, Phostoxin, and Primo-ban-20 were tested. Essential oil chemical composition was analyzed by GC-MS. M. lucida essential oil showed a high toxicological effect, producing 100% mortality after 72 hours at a dose of 0.20 μg/mL. M. lucida essential oil had a potent insecticidal activity (LC90 = 0.629 μg/mL) compared to both pesticides, Phostoxin (LC90 = 0.652 μg/mL) and Primo-ban-20 (LC90 = 0.726 μg/mL), at 24 h. The main compounds of the essential oil were the oxygenated monoterpenoids, 1,8-cineole (43.4%), and α-terpinyl acetate (14.5%), and the monoterpene hydrocarbons, mostly sabinene (8.2%) and β-pinene (4.0%). Results clearly indicate that M. lucida essential oil can be used as an effective alternative for pulse beetle C. maculatus control, and it could be tested against other pulse beetles affecting Asia and Africa and throughout the world, thereby reducing use of synthetic pesticides.

  16. Insecticidal Activity and Chemical Composition of the Morinda lucida Essential Oil against Pulse Beetle Callosobruchus maculatus

    Directory of Open Access Journals (Sweden)

    Moses S. Owolabi

    2014-01-01

    Full Text Available Insecticidal activity of essential oil extracted from Morinda lucida was tested on pulse beetle Callosobruchus maculatus, which is a pest that causes serious damage to several pulses. The insecticidal activity was compared with two pesticides, Phostoxin and Primo-ban-20. 120 mixed sex adult C. maculatus were introduced, along with 30 g of cowpeas. Four concentrations (0.40, 0.20, 0.10, and 0.05 μg/mL of the M. lucida essential oil, Phostoxin, and Primo-ban-20 were tested. Essential oil chemical composition was analyzed by GC-MS. M. lucida essential oil showed a high toxicological effect, producing 100% mortality after 72 hours at a dose of 0.20 μg/mL. M. lucida essential oil had a potent insecticidal activity (LC90 = 0.629 μg/mL compared to both pesticides, Phostoxin (LC90 = 0.652 μg/mL and Primo-ban-20 (LC90 = 0.726 μg/mL, at 24 h. The main compounds of the essential oil were the oxygenated monoterpenoids, 1,8-cineole (43.4%, and α-terpinyl acetate (14.5%, and the monoterpene hydrocarbons, mostly sabinene (8.2% and β-pinene (4.0%. Results clearly indicate that M. lucida essential oil can be used as an effective alternative for pulse beetle C. maculatus control, and it could be tested against other pulse beetles affecting Asia and Africa and throughout the world, thereby reducing use of synthetic pesticides.

  17. Liquid chromatographic determination of naringin and neohesperidin as a detector of grapefruit juice in orange juice.

    Science.gov (United States)

    Rouseff, R L

    1988-01-01

    Naringin/neohesperidin ratios can be used to differentiate orange juice which may contain added grapefruit juice from orange juice which may include juices from other naringin-containing cultivars. The naringin/neohesperidin ratios in juice vary from 14 to 83 in grapefruit (C. grandis) and from 1.3 to 2.5 in sour orange (C. aurantium) cultivars; the ratio is always less than 1 for the K-Early tangelo. Concentrations of both naringin and neohesperidin can be determined in orange juice by using a single liquid chromatographic isocratic reverse-phase system with a C-18 column. The detection limit for both compounds is 1 ppm with a linear working range to 500 ppm. Concentration relative standard deviations range from 0.47 to 1.06% for naringin and from 0.4 to 1.27% for neohesperidin. Naringin and neohesperidin recoveries ranged from 93 to 102% at concentrations of 5 and 50 ppm. Naringin values from blind duplicate samples of orange/grapefruit juice blends could be duplicated to +/- 3%.

  18. 78 FR 47006 - Lemon Juice From Argentina and Mexico

    Science.gov (United States)

    2013-08-02

    ... COMMISSION Lemon Juice From Argentina and Mexico Determination On the basis of the record \\1\\ developed in... investigation on lemon juice from Mexico would not be likely to lead to continuation or recurrence of material... USITC Publication 4418 (July 2013), entitled Lemon Juice from Argentina and Mexico: Investigation...

  19. 78 FR 46610 - Lemon Juice From Argentina and Mexico

    Science.gov (United States)

    2013-08-01

    ... COMMISSION Lemon Juice From Argentina and Mexico Determination On the basis of the record \\1\\ developed in... investigation on lemon juice from Mexico would not be likely to lead to continuation or recurrence of material... USITC Publication 4418 (July 2013), entitled Lemon Juice from Argentina and Mexico: Investigation...

  20. Determination of copper in clarified apple juices.

    Science.gov (United States)

    Zeiner, Michaela; Juranović Cindrić, Iva; Kröppl, Michaela; Stingeder, Gerhard

    2010-03-24

    Inorganic copper compounds are not considered as synthetic fertilizers for apple trees as they are traditional fertilizers. Thus, they are used in organic farming for soil or foliar applications. The European Union is for health reasons interested in reducing copper in apple orchards. Because the fertilizer application rate affects the nutrition of apples, the applied copper might also be reflected in the copper concentration of apple juices. Thus, the determination of copper is of concern for investigating the application of copper-containing fertilizers. Samples of clarified apple juice commercially available in the European market were analyzed for their copper content. Prior to quantification by inductively coupled plasma-optical emission spectrometry, the juices were processed by a microwave-assisted digestion system using HNO(3). All samples were also measured directly after dilution with HNO(3). The copper concentrations measured using both methods were all below the limit of detection (17 microg/L).

  1. Gastroprotective and Anti-ulcer activity of Aloe vera juice, Papaya fruit juice and Aloe vera and Papaya fruit combined juice in Ethanol induced Ulcerated Rats

    Directory of Open Access Journals (Sweden)

    S. Gopinathan

    2013-12-01

    Full Text Available Peptic ulcer is the most prevalent gastrointestinal disease. Even though a wide range of drugs are available for the treatment of peptic ulcer, but many of these do not fulfill all the requirements and have side effects. These factors have attracted researchers to investigate the natural products which have more efficacy, less side effects and less expensive for the treatment of peptic ulcer disease. In the present study the anti ulcer activity of (1 Aloe vera juice, (2 papaya fruit juice (3 Aloe vera and papaya fruit combined juice were investigated in the ethanol induced ulcerated rats. The administration of plant juices decreased the offensive factors like ulcer index and acid secretion and also reduced the amount of protein and carbohydrates in the stomach fluid. Further, plant juices increased the defensive factors like activity of oxidative enzymes such as superoxide dismutase and reduced glutathione. Activities of alkaline phosphatase and lipid peroxide were higher in the diseased condition and same were reduced after the treatment with plant juices. Content of haemoglobin and RBC and WBC counts were brought back to normalcy after the treatment with plant juices. The efficacy of plant juices was comparable with the reference drug- Ranitidine. The results of the present study reveal that the plant juices are having efficiency in the gastroprotective activity. It is recommended that the above said plant juices can be further studied for their anti ulcer efficacy in human subjects.

  2. Ochratoxin A in grape juice and wine.

    Science.gov (United States)

    Woese, K

    2000-03-01

    37 different grape juices and 21 red wines were analyzed for their contents of ochratoxin A at the State's Veterinary and Food Research Institute of Saxony-Anhalt in Halle in 1999 and the 1(st) quarter of 2000. Ochratoxin A was found in 78 % of the grape juices and 100 % of the wines. 27 % or 33 % respectively exceeded the EC Commission's lately intended proposal for an ochratoxin A maximum permissible level which is 0.5 ppb. A higher contamination rate for wines originating from southern growing regions of the EC was noticeable.

  3. Clarification of purple carrot juice: analysis of the fouling mechanisms and evaluation of the juice quality.

    Science.gov (United States)

    Ennouri, Monia; Ben Hassan, Ines; Ben Hassen, Hanen; Lafforgue, Christine; Schmitz, Philippe; Ayadi, Abdelmoneim

    2015-05-01

    Purple carrot juice was clarified by microfiltration. Two modes of filtration, batch concentration and total recycle were tested and the effect of microfiltration process on permeate flux and membrane fouling was studied. Intrinsic membrane resistance was negligible compared with the fouling resistances, which was less than 5 % of total resistance. Determination of membrane hydraulic permeability showed that water cleaning could permit a recovery of about 7 % of initial hydraulic flux. The analysis of color parameters of feed, permeate and concentrate juice during filtration shows that the a* and b* values decrease for the permeate corresponding respectively to changes from green to red and from blue to yellow. The total sugar and reducing sugars increase in permeate and decrease in concentrate. This work showed that it was possible to clarify the purple carrot juice by microfiltration with a real amelioration of the juice appearance.

  4. In vitro demineralization of enamel by orange juice, apple juice, Pepsi Cola and Diet Pepsi Cola.

    Science.gov (United States)

    Grobler, S R; Senekal, P J; Laubscher, J A

    1990-12-01

    Enamel demineralization was studied over periods related to normal use of an orange juice, an apple juice, Pepsi Cola and Diet Pepsi Cola. Rectangular blocks of intact human enamel (3 mm x 3 mm) were cut from teeth, coated with nail varnish except for the enamel surface and exposed to the drinks for 2, 4, 5, 6 or 40 minutes. The amount of calcium released from the enamel into solution was determined with the use of an atomic absorption spectrophotometer. The results showed the following degree of enamel demineralization: Pepsi Cola = orange juice greater than apple juice greater than Diet Pepsi Cola. The results suggest that diet colas are less demineralizing than other acid drinks, and complementary plaque studies indicate that they are also less cariogenic. The study emphasized the importance of acid-type, buffer capacity, pH and the presence of other components on the degree of enamel demineralization.

  5. Chemical characterization of tomato juice fermented with bifidobacteria.

    Science.gov (United States)

    Koh, Jong-Ho; Kim, Youngshik; Oh, Jun-Hyun

    2010-06-01

    The objective of this research was to characterize the chemical properties of tomato juice fermented with bifidobacterial species. Tomato juice was prepared from fresh tomatoes and heated at 100 degrees C prior to fermentation. Bifidobacterium breve, Bifidobacterium longum, and Bifidobacterium infantis were inoculated in tomato juice and kept at 35 to 37 degrees C for up to 6 h. Fructooligosaccharide (FOS) was added to tomato juice prior to fermentation. The analyses for brix, total titratable acidity (TTA), pH, color, and lycopene content were conducted to characterize tomato juices fermented with bifidobacterial species. Heat treatment of tomato juice did not cause any significant changes in brix, pH, and TTA. Only the redness of tomato juice was significantly increased, as the heating time increased to 30 min. The tomato juices fermented with B. breve and B. longum exhibited significant decreases in pH (3.51 and 3.80, respectively) and significant increases in TTA (13.50 and 12.50, respectively) (P tomato juice. The addition of FOS further improved the fermentation of tomato juice by bifidobacterial species. The lycopene contents of tomato juice were significantly increased from 88 to 113 microg/g by heat treatment at 100 degrees C (P < 0.05), however did not exhibit any significant change after fermentation with bifidobacterial species.

  6. Antioxidant activity of polyphenol-enriched apple juice

    Directory of Open Access Journals (Sweden)

    Šumić Zdravko M.

    2009-01-01

    Full Text Available This paper shows that it is possible to improve antioxidant activity of apple juice by extraction of polyphenolic compounds from apple pomace, as waste, and their addition to the apple juice. Raw apple juice was prepared by pressing of apple mash. After thermal treatment of raw apple juice, depectinisation, additional clarification and filtration, the clarified juice was obtained. In raw and clarified apple juice soluble solids, acidity, reducing sugar, total sugars and brown component content were determined, as well as total dry matter, ash, acidity, reducing sugar, total sugars, total pectins, cellulose and starch content in apple mash and pomace. The total cotent of phenolics in clarified apple juice and apple pomace extract, determined spectrophotometrically using the Folin- Ciocalteu reagent, was 0.496 mg/ml and 6.505 mg/g, respectively. The antioxidant activity of clarified and polyphenol-enriched clarified juice (with addition of apple pomace extract in the concentrations 0.05 g, 0.1 g, 0.5 g and 1 g of phenolic compounds per liter of clarified apple juice was examined on stable 1,1-diphenyl-2-picrylhydrazyl (DPPH free radicals. Based on the obtained results it can be concluded that polyphenol-enriched clarified juice was more effective on DPPH radicals than the clarified apple juice.

  7. Quality assessment of fruit juices by NIR spectroscopy

    Directory of Open Access Journals (Sweden)

    Petr Šnurkovič

    2013-01-01

    Full Text Available In the article there is described the detection of substances used for adulteration of fruit juices. They were investigated in juice products distributed within the network of retail markets. This detection was performed using the method of near-infrared spectroscopy (NIR. There were analysed samples of orange juices, apple juices, and grapefruit juices. At the beginning, there were assessed quality parameters (soluble dry matter, total acids, formol number, malic acid, citric acid, ascorbic acid, lactic acid, specific density, and ethanol content and their compatibility with the limit values specified by the Association of the Industries of Juices and Nectars. The research covered 45 kinds of fruit juices and there was found 10 cases when at least one parameter was out of the approved limits. After that there was used the discrimination analysis and it enabled to separate pure juices from those containing at least 1 % of admixtures. This method also helped to distinguish between the group of fresh squeezed juices and the juices made of fruit concentrates. Using the diagnostics “Principal Component Scores 3D Display”, there were arranged various numbers of similar groups of juice samples with similar compound profiles.

  8. Phlorizin and sorbitol in Vitis labrusca grape juices

    Directory of Open Access Journals (Sweden)

    Spinelli Fernanda Rodrigues

    2016-01-01

    Full Text Available In Brazil, grape juice is a not fermented beverage, made from Vitis labrusca varieties and their hybrids. The most common form of adulteration is by the addition of apple juice. The adulterated samples can be identified by specific analysis, since apple juice has some compounds that grape has not. A more simplified and assessible way to determine this kind of adulteration is the phlorizin analysis by high performance liquid chromatography (HPLC. Phlorizin is a phenolic compound that has been used to identify adulterations in juices. Besides phlorizin, it can be seen that adulteration by quantifying the levels of sorbitol, present in apples, but absent in healthy grapes. The content of this compounds in grape juices can vary due to the variety of grape and harvest.This study aimed to analyze phlorizin and sorbitol, in 141 experimental samples of Vitis labrusca grape juices, harvest 2016, of 9 varieties and 43 commercial samples (different vintages. The experimental juices from red grapes showed higher sorbitol content than the white grapes. For sorbitol analysis, the juices of Ives differed statistically of the juices of: Isabella, Concord, Niagara Branca and White Muscat. It was detected the presence of apple juice in 5 commercial grape juices.

  9. Fruit Juice in Infants, Children, and Adolescents: Current Recommendations.

    Science.gov (United States)

    Heyman, Melvin B; Abrams, Steven A

    2017-06-01

    Historically, fruit juice was recommended by pediatricians as a source of vitamin C and as an extra source of water for healthy infants and young children as their diets expanded to include solid foods with higher renal solute load. It was also sometimes recommended for children with constipation. Fruit juice is marketed as a healthy, natural source of vitamins and, in some instances, calcium. Because juice tastes good, children readily accept it. Although juice consumption has some benefits, it also has potential detrimental effects. High sugar content in juice contributes to increased calorie consumption and the risk of dental caries. In addition, the lack of protein and fiber in juice can predispose to inappropriate weight gain (too much or too little). Pediatricians need to be knowledgeable about juice to inform parents and patients on its appropriate uses. Copyright © 2017 by the American Academy of Pediatrics.

  10. Microbiological quality of fresh nopal juice

    Science.gov (United States)

    Microbial quality of commercially available fresh juices (n=162) made with nopal cactus in Texcoco, State of Mexico, was evaluated during the summer and spring season. All samples contained total coliforms and 91% were positive for Escherichia coli. Although total coliforms and E. coli were detected...

  11. The Multi - vitamin Nutrient Fresh Vegetable Juice

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    @@ In the Tenth Five- Year Plan period, an important strategy for food industry is to develop fruit vegetable freshening and processing industry. Now,the consumable demand for vegetables turns to fresh,convenient, nutritious, safe and dean ones, while semi-processed vegetables and mixed fresh vegetable juices will meet this market demand exactly.

  12. 21 CFR 146.132 - Grapefruit juice.

    Science.gov (United States)

    2010-04-01

    ..._locations.html. The food may contain one or any combination of the optional ingredients specified in... 21 Food and Drugs 2 2010-04-01 2010-04-01 false Grapefruit juice. 146.132 Section 146.132 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) FOOD FOR...

  13. 巴戟天寡糖抗抑郁作用的研究进展%Research advance of morinda officinalis oligosaccharides in treatment of depression

    Institute of Scientific and Technical Information of China (English)

    邹连勇; 张鸿燕

    2012-01-01

    Traditional Chinese medicine Morinda officinalis How has complicated chemical constituents and pharmacological effects. In the recent studies on the pharmacological effects of morinda officinalis how by modern pharmacological methods, it was found that its extracts had antidepressant activities in several kinds of animal depression models, and the main ingredients were considered as bajitian oligosaccharide. In addition, bajitian oligo-saccharide also could protect the nerve cells from the damage of corticosterone. Confirmed by a number of clinical trials, morinda officinalis oligose capsules have similar effectiveness to fluoxetine in depressive disorder with mild adverse reactions. This article reviewed the progress of the studies on the antidepressant effects and toxicology of morinda officinalis how.%中药巴戟天化学成分及药理作用复杂.近年来采用现代药理学方法研究巴戟天的药理作用,发现巴戟天提取物在多种抑郁动物模型中具有抗抑郁效果,其主要成分为一组菊淀粉型寡糖同系物.另外,巴戟天寡糖还能够保护神经细胞免受皮质酮的损伤.已有多项临床试验证实巴戟天寡糖胶囊能够治疗轻中度抑郁症,疗效与氟西汀相当,且不良反应较轻.本研究综述目前巴戟天的毒理学及抗抑郁研究进展.

  14. Commercial scale pulsed electric field processing of tomato juice.

    Science.gov (United States)

    Min, Seacheol; Jin, Z Tony; Zhang, Q Howard

    2003-05-21

    Effects of commercial scale pulsed electric field (PEF) processing on the quality of tomato juice were studied and compared with those of thermal processing. Tomato juice was prepared by hot break at 88 degrees C for 2 min or by cold break at 68 degrees C for 2 min and then thermally processed at 92 degrees C for 90 s or PEF processed at 40 kV/cm for 57 micros. Thermally processed, PEF processed, and unprocessed control juices were packed into 50 mL sterilized polypropylene tubes in a sanitary glovebox and stored at 4 degrees C for 112 days. Both thermally and PEF processed juices showed microbial shelf life at 4 degrees C for 112 days. The lipoxygenase activities of thermally and PEF processed juices were 0 and 47%, respectively. PEF processed juice retained more ascorbic acid than thermally processed juice at 4 degrees C for 42 days (p Brix, pH, or viscosity between thermally and PEF processed juices during the storage (p > 0.05). Sensory evaluations indicated that flavor and overall acceptability of PEF processed juice were preferred to those of thermally processed juice (p < 0.05).

  15. Habitual intake of fruit juice predicts central blood pressure.

    Science.gov (United States)

    Pase, Matthew P; Grima, Natalie; Cockerell, Robyn; Pipingas, Andrew

    2015-01-01

    Despite a common perception that fruit juice is healthy, fruit juice contains high amounts of naturally occurring sugar without the fibre content of the whole fruit. Frequent fruit juice consumption may therefore contribute to excessive sugar consumption typical of the Western society. Although excess sugar intake is associated with high blood pressure (BP), the association between habitual fruit juice consumption and BP is unclear. The present study investigated the association of fruit juice consumption with brachial and central (aortic) BP in 160 community dwelling adults. Habitual fruit juice consumption was measured using a 12 month dietary recall questionnaire. On the same day, brachial BP was measured and central (aortic) BP was estimated through radial artery applanation. Frequency of fruit juice consumption was classified as rare, occasional or daily. Those who consumed fruit juice daily, versus rarely or occasionally, had significantly higher central systolic BP (F (2, 134) = 6.09, p fruit juice daily rather than rarely or occasionally. In conclusion, more frequent fruit juice consumption was associated with higher central BPs.

  16. Pasta Fortified with Potato Juice: Structure, Quality, and Consumer Acceptance.

    Science.gov (United States)

    Kowalczewski, Przemysław; Lewandowicz, Grażyna; Makowska, Agnieszka; Knoll, Ismena; Błaszczak, Wioletta; Białas, Wojciech; Kubiak, Piotr

    2015-06-01

    The potential of potato juice in relieving gastrointestinal disorders has already been proven. Work continues on implementation of this active component into products that are widely consumed. In this article, results of an attempt to fortify pasta with potato juice are presented and discussed. Fortification is performed using fresh and dried juice. The influence of the addition on culinary properties of the final product, such as cooking weight and cooking loss, as well as microstructure, color, texture, and consumer acceptance were evaluated. It was found that potato juice can be used for fortification of pasta both in its fresh and dried forms, however the effects on different responses depend on the potato juice form used. The addition of potato juice influenced the color of the product reducing its lightness and shifting color balances from green to red, yellow color saturation was decreased as well. Changes in color were more significant in the case of fresh juice addition. The firmness and microstructure of pasta was also influenced. The surface microstructure of pasta containing fresh potato juice was different from that of the other 2 products being a likely explanation of the lower cooking loss observed in its case. In contrast, the consistency of dough was strengthened by addition of dried potato juice. Principal components analysis indicated that the color change had the most pronounced effect on consumer acceptance. Other physicochemical changes were slightly less significant. Nevertheless, sensory evaluation proved that functional pasta produced with fresh potato juice finds consumer acceptance comparable with that of classic pasta.

  17. Microbes Associated with Freshly Prepared Juices of Citrus and Carrots

    Directory of Open Access Journals (Sweden)

    Kamal Rai Aneja

    2014-01-01

    Full Text Available Fruit juices are popular drinks as they contain antioxidants, vitamins, and minerals that are essential for human being and play important role in the prevention of heart diseases, cancer, and diabetes. They contain essential nutrients which support the growth of acid tolerant bacteria, yeasts, and moulds. In the present study, we have conducted a microbiological examination of freshly prepared juices (sweet lime, orange, and carrot by serial dilution agar plate technique. A total of 30 juice samples were examined for their microbiological quality. Twenty-five microbial species including 9 bacterial isolates, 5 yeast isolates, and 11 mould isolates were isolated from juices. Yeasts and moulds were the main cause of spoilage of juices. Aspergillus flavus and Rhodotorula mucilaginosa were observed in the maximum number of juice samples. Among bacteria Bacillus cereus and Serratia were dominant. Escherichia coli and Staphylococcus aureus were detected in few samples. Candida sp., Curvularia, Colletotrichum, and Acetobacter were observed only in citrus juice samples. Alternaria, Aspergillus terreus, A. niger, Cladosporium, and Fusarium were also observed in tested juice samples. Some of the microorganisms detected in these juice samples can cause disease in human beings, so there is need for some guidelines that can improve the quality of fruit juices.

  18. Fluoride content of soft drinks, nectars, juices, juice drinks, concentrates, teas and infusions marketed in Portugal.

    Science.gov (United States)

    Fojo, C; Figueira, M E; Almeida, C M M

    2013-01-01

    A potentiometric method using a fluoride combination ion-selective electrode was validated and used to analyse 183 samples, including soft drinks, juices, nectars, juice drinks, concentrates, teas and infusions marketed in Portugal. The fluoride levels were higher in extract-based soft drinks, juice drinks and juice, with fluoride values of 0.86 ± 0.35, 0.40 ± 0.24 and 0.37 ± 0.11 mg l⁻¹, respectively. The lowest fluoride concentration was found in infusion samples (0.12 ± 0.01 mg l⁻¹), followed by teas and carbonated soft drinks with fluoride concentrations of 0.16 ± 0.12 and 0.18 ± 0.07 mg l⁻¹, respectively. Nectars, concentrates and juice-based drinks had similar fluoride concentrations of 0.33 ± 0.16, 0.29 ± 0.12 and 0.25 ± 0.14 mg l⁻¹, respectively. The fluoride concentrations in all these samples would only contribute intakes below the acceptable daily intake (ADI = 0.05 mg kg⁻¹ body weight day⁻¹), indicating that, individually, these beverages cannot induce fluoride toxicity in the population group of children.

  19. Analytical characterization of some pasteurized apple juices during storage

    Directory of Open Access Journals (Sweden)

    Damian Cristina

    2015-06-01

    Full Text Available The aim of the study was to assess the effect of three weeks of storage on the chemical and rheological properties of apple juices obtained from Idared and Jonatan apples variety. Total antioxidant activity, levels of bio-active compound groups and the viscosity were measured to characterize the investigated juices. The method applied for the determination of ascorbic acid concentration was with 2, 6-diclorophenolindophenol. Total phenols (TP in apple juices were determined using the Folin-Ciocalteau method and antioxidant activity by the use of DPPH free radical method. The viscosity of apple juices was investigated by a rotational viscometer, Brookfield viscometer (Brookfield Engineering Inc., Model RV-DV I Prime with RV spindles. During three weeks of storage, different rates of all measured properties have been observed decreasing for both studied apples varieties juices. The juices from Jonatan apples have higher antioxidant activities that are correlated with the higher content in polyphenols and lower values of viscosity.

  20. Statement on ‘toothkind’ juice drinks

    DEFF Research Database (Denmark)

    Tetens, Inge

    2011-01-01

    or sugar-containing non-alcoholic beverages with an equivalent number of servings of „toothkind‟ juice drink. In the context of the claim, „reduction of tooth demineralisation‟ has a similar meaning to „maintenance of tooth mineralisation‟. © European Food Safety Authority, 2011.......Following a request from the European Commission, the Panel on Dietetic Products, Nutrition and Allergies was asked to deliver advice in relation to „toothkind‟ juice drinks. The Panel was requested to consider whether conclusions for the substantiation of the beneficial effect, i.e. reduction...... of tooth demineralisation, can be drawn on the basis of studies which do not measure directly net demineralisation but measure the potential for demineralisation of enamel, e.g. reduced pH on dental plaque. The Panel considers that reduction of plaque pH immediately (within about 10 min) following a single...

  1. Comprehensive proteomic analysis of human pancreatic juice

    DEFF Research Database (Denmark)

    Grønborg, Mads; Bunkenborg, Jakob; Kristiansen, Troels Zakarias

    2004-01-01

    ). In addition, we identified a number of proteins that have not been previously described in pancreatic juice (e.g., tumor rejection antigen (pg96) and azurocidin). Interestingly, a novel protein that is 85% identical to HIP/PAP was identified, which we have designated as PAP-2. The proteins identified...... in this study could be directly assessed for their potential as biomarkers for pancreatic cancer by quantitative proteomics methods or immunoassays....

  2. Phlorizin and sorbitol in Vitis labrusca grape juices

    OpenAIRE

    Spinelli Fernanda Rodrigues; Dutra Sandra Valduga; Leonardelli Susiane; Carnieli Gilberto João; Vanderlinde Regina

    2016-01-01

    In Brazil, grape juice is a not fermented beverage, made from Vitis labrusca varieties and their hybrids. The most common form of adulteration is by the addition of apple juice. The adulterated samples can be identified by specific analysis, since apple juice has some compounds that grape has not. A more simplified and assessible way to determine this kind of adulteration is the phlorizin analysis by high performance liquid chromatography (HPLC). Phlorizin is a phenolic compound that has been...

  3. Bioethanol production from fermentable sugar juice.

    Science.gov (United States)

    Zabed, Hossain; Faruq, Golam; Sahu, Jaya Narayan; Azirun, Mohd Sofian; Hashim, Rosli; Boyce, Amru Nasrulhaq

    2014-01-01

    Bioethanol production from renewable sources to be used in transportation is now an increasing demand worldwide due to continuous depletion of fossil fuels, economic and political crises, and growing concern on environmental safety. Mainly, three types of raw materials, that is, sugar juice, starchy crops, and lignocellulosic materials, are being used for this purpose. This paper will investigate ethanol production from free sugar containing juices obtained from some energy crops such as sugarcane, sugar beet, and sweet sorghum that are the most attractive choice because of their cost-effectiveness and feasibility to use. Three types of fermentation process (batch, fed-batch, and continuous) are employed in ethanol production from these sugar juices. The most common microorganism used in fermentation from its history is the yeast, especially, Saccharomyces cerevisiae, though the bacterial species Zymomonas mobilis is also potentially used nowadays for this purpose. A number of factors related to the fermentation greatly influences the process and their optimization is the key point for efficient ethanol production from these feedstocks.

  4. Bioethanol Production from Fermentable Sugar Juice

    Science.gov (United States)

    Zabed, Hossain; Faruq, Golam; Sahu, Jaya Narayan; Azirun, Mohd Sofian; Hashim, Rosli; Nasrulhaq Boyce, Amru

    2014-01-01

    Bioethanol production from renewable sources to be used in transportation is now an increasing demand worldwide due to continuous depletion of fossil fuels, economic and political crises, and growing concern on environmental safety. Mainly, three types of raw materials, that is, sugar juice, starchy crops, and lignocellulosic materials, are being used for this purpose. This paper will investigate ethanol production from free sugar containing juices obtained from some energy crops such as sugarcane, sugar beet, and sweet sorghum that are the most attractive choice because of their cost-effectiveness and feasibility to use. Three types of fermentation process (batch, fed-batch, and continuous) are employed in ethanol production from these sugar juices. The most common microorganism used in fermentation from its history is the yeast, especially, Saccharomyces cerevisiae, though the bacterial species Zymomonas mobilis is also potentially used nowadays for this purpose. A number of factors related to the fermentation greatly influences the process and their optimization is the key point for efficient ethanol production from these feedstocks. PMID:24715820

  5. The Branding of Sugarcane Juice in India

    Directory of Open Access Journals (Sweden)

    R. Sinha

    2014-06-01

    Full Text Available Sugarcane juice is traditionally sold in India by roadside vendors, often in unhygienic conditions. That’s why a few entrepreneurs have taken the initiative venturing into the marketing of branded sugarcane juice through a chain of franchised outlets. Initial indications are that this model is headed for success. Pune, Kolhapur, more known for its leather chappals, has also been blessed with an abundance of milk, water and sugar, which has made the region the nation's kitchen for many years. The Warana milk producers' cooperative located here has lived up to this reputation. It has been a contract manufacturer for products such as Cadbury's Bournvita, butter for Britannia Industries and Soya milk for Ruchi Soya. Now, the cooperative is preparing to assert its own identity through the launch of Warana Joy, its national brand. Among its new products is sugarcane juice in aseptic packs (Tetra Pak. This article outlines the development of this business; the opportunities and threats faced and also offer suggestions for the growth in this market.

  6. Study on the development of pomegranate juice processing technology: clarification of pomegranate juice.

    Science.gov (United States)

    Vardin, Hasan; Fenercioğlu, Hasan

    2003-10-01

    The effects of clarification agents and methods on pomegranate juice quality were studied. Fruits were pressed as whole, and extracted juice was clarified with gelatin, polyvinylypolypyrrolidone (PVPP) and natural sedimentation. In order to determine the effects of these clarifying techniques on product quality, pH, total phenolic substances (TPS), anthocyanin, turbidity, and total colour density tests were applied to each sample. In clarification, the main purpose was to reduce the amount of phenolic substances. Phenolic substances were controlled in each clarification method. The most effective method was the application of 1 g/L gelatine for clarification. Sensory evaluation also applied for clarified juices and gelatin application was selected by panelists to be superior.

  7. Diabetic Wound Healing and Activation of Nrf2 by Herbal Medicine

    Science.gov (United States)

    Senger, Donald R.; Cao, Shugeng

    2016-01-01

    Nrf2 defense is a very important cellular mechanism to control oxidative stress, which is implicated in wound healing. Nrf2 can induce many cytoprotective genes, including HO-1, NQO1 and G6PD. Among many natural products that have been reported as Nrf2 activators, sulforaphane and curcumin have been studied more widely than any others, and both are in clinical trials for non-cancerous disorders. Recently, we reported 4-ethyl catechol and 4-vinyl catechol as Nrf2 co-factors that can induce Nrf2 as potently as sulforaphane and curcumin. These new Nrf2 co-factors were identified in hot aqueous extract of an herbal medicine Barleria lupulina, and fermented Noni (Morinda citrifolia) juice, which are used traditionally for diabetic wound healing. PMID:27868087

  8. Detection of some intestinal protozoa in commercial fresh juices.

    Science.gov (United States)

    Mossallam, Shereen F

    2010-04-01

    Fresh fruit juices are popular, but not always safe. For assessing the likelihood of infection with newly emerging intestinal protozoa, commercial fresh orange, lemon, sugar cane, strawberry, and mango juices were screened by wet mounts, Weber's modified trichrome and modified Ziehl-Neelsen stains. Protozoa viability was done by fluorescein-diacetate/propidium-iodide staining, and infectivity was performed in Swiss albino mice. Results showed that 35.43% were contaminated with one or more of Cryptosporidia, Microsporidia, and Cyclospora, as well as Giardia spp. Strawberry was the most contaminated juice (54.28%), while orange was the slightest (22.86%). Cryptosporidia was the highest contaminant (61.29%), and Cyclospora was the least (14.52%). Microsporidia spp. was the most robust contaminant which retained its viability and infectivity in juices in which it was detected. Moderately acidic strawberry and mango juices and alkaline sugar cane juice pose a possible threat, due to harboring the highest viable and infectious protozoa. Regarding highly acidic juices, viability and infectivity decreased in lemon, yet was not still risk free. Orange juice was comparatively safe, as viability dramatically declined, while infectivity was completely abolished. Hence consumers, especially high risk group, are placed at hazard of contracting intestinal protozoa infections, especially through moderately acidic and alkaline juices.

  9. The effects of tomato juice on male infertility.

    Science.gov (United States)

    Yamamoto, Yu; Aizawa, Koichi; Mieno, Makiko; Karamatsu, Mika; Hirano, Yasuko; Furui, Kuniko; Miyashita, Tatsuya; Yamazaki, Kazumitsu; Inakuma, Takahiro; Sato, Ikuo; Suganuma, Hiroyuki; Iwamoto, Teruaki

    2017-01-01

    This study aimed to investigate the effects of tomato juice consumption on seminal plasma lycopene levels and sperm parameters in infertile men. Subjects were male infertility patients with poor sperm concentration (juice group, an antioxidant group, and a control group. The subjects in the tomato juice group and the antioxidant group daily consumed one can of tomato juice (containing 30 mg of lycopene) or one antioxidant capsule (containing vitamin C 600 mg, vitamin E 200 mg, and glutathione 300 mg), respectively, for 12 weeks (feeding period). Seminal plasma lycopene levels and sperm parameters were measured every 6 weeks during the feeding period. Forty-four patients completed the study (control group: 12, antioxidant group: 15, tomato juice group: 17). In the tomato juice group, plasma lycopene level was significantly increased at the 12th week of the feeding period. Moreover, a decrease in seminal plasma white blood cells and an increase in sperm motility in the tomato juice group were statistically significant at the 12th and 6th weeks, respectively, compared to the control group. In the antioxidant capsule group, no significant improvement was observed in semen parameters. In conclusion, regular consumption of tomato juice seems to improve sperm motility in infertile patients. This is the first report to show that commercially available food, such as tomato juice, might be beneficial for male infertility.

  10. The colligative properties of fruit juices by photopyroelectric calorimetry

    Science.gov (United States)

    Frandas, A.; Surducan, V.; Nagy, G.; Bicanic, D.

    1999-03-01

    The photopyroelectric method was used to study the depression of freezing point in juices prepared from selected apple and orange juice concentrates. By using the models for real solutions, the effective molecular weight of the dissolved solids was obtained. The acids concentration in the fruit juice is reflected both in the equivalent molecular weight (by lowering it) and in the interaction coefficients b and C. Using the data for the molecular weight and the characteristic coefficients, prediction curves for the samples investigated can be used in practice. Freezing point depression can also be used as an indicator of the degree of spoilage of fruit juices.

  11. Use of Moringa oleífera Lamarck leaf extract as sugarcane juice clarifier: effects on clarifed juice and sugar

    OpenAIRE

    Gustavo Henrique Gravatim Costa; Igor dos Santos Masson; Lidyane Aline de Freita; Juliana Pelegrini Roviero; Márcia Justino Rossini Mutton

    2014-01-01

    The objective of this study was to evaluate the effect of Moringa oleifera Lam. leaf extract on the sedimentation of impurities in the treatment of sugarcane juice and the effects on sugar quality and on the clarified juice. The experimental design used was a 4x2 factorial arrangement with four replications. The main treatments performed included the extracted original sugarcane juice, the synthetic polyelectrolyte (Flomex 9076), the leaf extract, and a control. The secondary treatments consi...

  12. Antibacterial Activity of the Isolation Ethyl Acetate-Soluble Extract Noni Fruit (Morindra citrifolia L.) against Meat Bacterial Decay

    Science.gov (United States)

    Nugraheni, E. R.; Nurrakhman, M. B. E.; Munawaroh, H.; Saputri, L.

    2017-02-01

    Noni (Morindra citrifolia L.) is native to Indonesia which have medicinal properties. One of them as an antibacterial. This study aims to determine the antibacterial activity of isolates from the ethanol extract noni fruit to bacterial decay meat is Bacillus licheniformis, Klebsiella pneumonia, Bacillus alvei, Acinetobacter calcoaceticus, and Staphylococcus saprophyticus. The extraction process using the maceration method, and then made a partition by centrifugation ethyl acetate. Soluble part partition showed bacterial growth inhibition activity of the strong to very strong. Furthermore, the ethyl acetate soluble partition on preparative thin layer chromatography produced 5 isolates. Isolates obtained antibacterial activity test performed with a concentration of 20% and 30%. The results of antibacterial test against bacteria test isolates, showing isolates A can not inhibit the growth of bacteria, isolates B and C have medium activity and strong, isolates D and E isolates have activity against bacteria that were tested. MIC and MBC test results showed that the isolates B gives an inhibitory effect (bacteriostatic) against all bacteria. Content analysis of compounds by TLC using the reagents cerium (IV) sulfate indicates a phenol group. Isolates B contains a major compound which can be used as an antibacterial candidate in food preservation replace chemical preservatives.

  13. Electrodialytic removal of nitrate from pineapple juice: effect on selected physicochemical properties, amino acids, and aroma components of the juice.

    Science.gov (United States)

    Ackarabanpojoue, Yuwadee; Chindapan, Nathamol; Yoovidhya, Tipaporn; Devahastin, Sakamon

    2015-05-01

    This study aimed at investigating the effect of nitrate removal from pineapple juice by electrodialysis (ED) on selected properties of the ED-treated juice. Single-strength pineapple juice with reduced pulp content was treated by ED to reduce the nitrate concentration to 15, 10, or 5 ppm. After ED, the removed pulp was added to the ED-treated juice and its properties, including electrical conductivity, acidity, pH, total soluble solids (TSS), color, amino acids, and selected aroma compounds, were determined and compared with those of the untreated juice. ED could reduce the nitrate content of 1 L of pineapple juice from an initial value of 50 ppm to less than 5 ppm within 30 min. A significant decrease in the electrical conductivity, acidity, pH, TSS, and yellowness, but a significant increase in the lightness, of the juice was observed upon ED. Concentrations of almost all amino acids of the ED-treated juice significantly decreased. The concentrations of 8 major compound contributors to the pineapple aroma also significantly decreased. Adding the pulp back to the ED-treated juice increased the amino acids concentrations; however, it led to a significant decrease in the concentrations of the aroma compounds.

  14. Stability of enterocin AS-48 in fruit and vegetable juices.

    Science.gov (United States)

    Grande, Maria J; Lucas, Rosario; Valdivia, Eva; Abriouel, Hikmate; Maqueda, Mercedes; Omar, Nabil Ben; Martínez-Cañamero, Magdalena; Gálvezi, Antonio

    2005-10-01

    Enterocin AS-48 is a candidate bacteriocin for food biopreservation. Before addressing application of AS-48 to vegetable-based foods, the interaction between AS-48 and vegetable food components and the stability of AS-48 were studied. Enterocin AS-48 had variable interactions with fruit and vegetable juices, with complete, partial, or negligible loss of activity. For some juices, loss of activity was ameliorated by increasing the bacteriocin concentration, diluting the juice, or applying a heat pretreatment. In juices obtained from cabbage, cauliflower, lettuce, green beans, celery, and avocado, AS-48 was very stable for the first 24 to 48 h of storage under refrigeration, and decay of activity was markedly influenced by storage temperature. In fresh-made fruit juices (orange, apple, grapefruit, pear, pineapple, and kiwi) and juice mixtures, AS-48 was very stable for at least 15 days at 4 degrees C, and bacteriocin activity was still detectable after 30 days of storage. Gradual and variable loss of activity occurred in juices stored at 15 and 28 degrees C; inactivation was faster at higher temperatures. In commercial fruit juices (orange, apple, peach, and pineapple) stored at 4 degrees C, the bacteriocin was completely stable for up to 120 days, and over 60% of initial activity was still present in juices stored at 15 degrees C for the same period. Commercial fruit juices stored at 28 degrees C for 120 days retained between 31.5% (apple) and 67.71% (peach) of their initial bacteriocin activity. Solutions of AS-48 in sterile distilled water were stable (120 days at 4 to 28 degrees C). Limited loss of activity was observed after mixing AS-48 with some food-grade dyes and thickening agents. Enterocin AS-48 added to lettuce juice incubated at 15 degrees C reduced viable counts of Listeria monocytogenes CECT 4032 and Bacillus cereus LWL1 to below detection limits and markedly reduced viable counts of Staphylococcus aureus CECT 976.

  15. The inner quality of orange juice and apple juice. Assessment by the use of microbiological methods.

    NARCIS (Netherlands)

    Ekasari, I.

    1989-01-01

    A modified Salmonella mutagenicity assay and a cytotoxicity test were developed to. determine the concentration of sensorially inactive Maillard Intermediate Products (MIP) in fruit juices. The formation of MIP is initiated by heat treatments necessary for pasteurization and thermal concentration. I

  16. The inner quality of orange juice and apple juice : assessment by the use of microbiological methods

    NARCIS (Netherlands)

    Ekasari, I.

    1989-01-01

    A modified Salmonella mutagenicity assay and a cytotoxicity test were developed to. determine the concentration of sensorially inactive Maillard Intermediate Products (MIP) in fruit juices. The formation of MIP is initiated by heat treatments necessary for

  17. The inner quality of orange juice and apple juice : assessment by the use of microbiological methods

    NARCIS (Netherlands)

    Ekasari, I.

    1989-01-01

    A modified Salmonella mutagenicity assay and a cytotoxicity test were developed to. determine the concentration of sensorially inactive Maillard Intermediate Products (MIP) in fruit juices. The formation of MIP is initiated by heat treatments necessary for pasteurizatio

  18. 21 CFR 146.152 - Orange juice with preservative.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 2 2010-04-01 2010-04-01 false Orange juice with preservative. 146.152 Section... any safe and suitable preservatives or combinations thereof. (c) The name of the food is “Orange juice with preservative”. (d) Label declaration. Each of the ingredients used in the food shall be...

  19. Pancreatic Juice Culture in Acute Pancreatitis and Other Pancreatic Disorders

    Directory of Open Access Journals (Sweden)

    Masataka Kikuyama

    2016-09-01

    Full Text Available We retrospectively evaluated the results of pancreatic juice cultures of patients with acute pancreatitis and other pancreatic disorders. Methods Twenty patients who underwent pancreatic juice culture were studied. Nine had acute pancreatitis due to alcohol (n=5, idiopathic causes (n=2, drugs (n=1, or gallstones (n=1, and remaining 11 had other pancreatic disorders such as an intraductal papillary mucin-producing neoplasm (n=3 and main pancreatic duct dilatation with a stricture due to a tumorous lesion suspected of pancreatic cancer (n=7 or chronic pancreatitis (n=1 without symptoms. Nasopancreatic drainage tubes were placed for pancreatic duct drainage in acute pancreatitis and for pancreatic juice cytology in other disorders. Pancreatic juice was obtained through the drainage tube and cultured. Results Pancreatic juice cultures were positive in all patients with acute pancreatitis for Staphylococcus epidermidis, Streptococcus species, and others. Six among 11 patients (54.5% with other disorders showed positive results for Escherichia coli, Streptococcus salivarius, and others. The rate of positive pancreatic juice cultures was significantly higher in acute pancreatitis (p=0.038. Seven of the 9 patients with acute pancreatitis were classified as having severe acute pancreatitis, and all survived treatment. Conclusions Pancreatic juice culture was highly positive in acute pancreatitis. Further study is needed to confirm the relationship between orally indigenous bacteria identified in the pancreatic juice and acute pancreatitis.

  20. Native protein recovery from potato fruit juice by ultrafiltration

    NARCIS (Netherlands)

    Zwijnenberg, Harmen Jan; Kemperman, Antonius J.B.; Boerrigter, M.E.; Lotz, Martin; Dijksterhuis, Jan F.; Koops, G.H.; Poulsen, Poul Emil

    2002-01-01

    Potato fruit juice, i.e. the stream resulting after the extraction of the starch from the potato, contains up to 2.5% [w/w] of proteins that are potentially valuable for the food market. However, today the recovery of protein from the potato fruit juice with reverse osmosis membranes results in a

  1. The effects of juice processing on black mulberry antioxidants

    NARCIS (Netherlands)

    Tomas, M.; Toydemir, G.; Boyacioglu, D.; Hall, R.D.; Beekwilder, M.J.; Capanoglu, E.

    2015-01-01

    Black mulberry fruit is processed to juice at significant scale in Turkey. The effect of industrial-scale juice production on black mulberry antioxidants was evaluated using samples collected from the main steps of processing; including the selection of fruits, washing, mechanical milling, mashing,

  2. Comparative proteomic analysis of human pancreatic juice : Methodological study

    NARCIS (Netherlands)

    Zhou, Lu; Lu, ZhaoHui; Yang, AiMing; Deng, RuiXue; Mai, CanRong; Sang, XinTing; Faber, Klaas Nico; Lu, XingHua

    Pancreatic cancer is the most lethal of all the common malignancies. Markers for early detection of this disease are urgently needed. Here, we optimized and applied a proteome analysis of human pancreatic juice to identify biomarkers for pancreatic cancer. Pancreatic juice samples, devoid of blood

  3. Bioactive and functional properties of sour cherry juice (Prunus cerasus).

    Science.gov (United States)

    Cásedas, Guillermo; Les, Francisco; Gómez-Serranillos, Maria Pilar; Smith, Carine; López, Víctor

    2016-11-09

    Sour cherry juice (Prunus cerasus) is consumed as a nutritional supplement claiming health effects. The aim of the study was to evaluate the different properties of sour cherry juice in terms of antioxidant activity and inhibition of target enzymes in the central nervous system and diabetes. The content of polyphenols and anthocyanins was quantified. Different experiments were carried out to determine the radical scavenging properties of the juice. The activity of sour cherry juice was also tested in physiological relevant enzymes of the central nervous system (acetylcholinesterase, monoamine oxidase A, tyrosinase) and others involved in type 2 diabetes (α-glucosidase, dipeptidyl peptidase-4). Sour cherry juice showed significant antioxidant effects but the activity of the lyophilized juice was not superior to compounds such as ascorbic, gallic or chlorogenic acid. Furthermore, sour cherry juice and one of its main polyphenols known as chlorogenic acid were also able to inhibit monoamine oxidase A and tyrosinase as well as enzymes involved in diabetes. This is the first time that sour cherry juice is reported to inhibit monoamine oxidase A, α-glucosidase and dipeptidyl peptidase-4 in a dose dependent manner, which may be of interest for human health and the prevention of certain diseases.

  4. 27 CFR 24.241 - Decolorizing juice or wine.

    Science.gov (United States)

    2010-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2010-04-01 2010-04-01 false Decolorizing juice or wine..., DEPARTMENT OF THE TREASURY LIQUORS WINE Storage, Treatment and Finishing of Wine § 24.241 Decolorizing juice or wine. (a) Conditions and limitations. If the proprietor wishes to use activated carbon or...

  5. The mechanism of cloud loss phenomena in orange juice

    NARCIS (Netherlands)

    Krop, J.J.P.

    1974-01-01

    The importance of many factors for the cloud stability of orange juice was investigated. By the determination of methanol, cloud loss of orange juice could be ascribed directly to the action of pectin esterase. However, clarification only occurs if calcium ions are available to precipitate the low m

  6. The effects of juice processing on black mulberry antioxidants

    NARCIS (Netherlands)

    Tomas, M.; Toydemir, G.; Boyacioglu, D.; Hall, R.D.; Beekwilder, M.J.; Capanoglu, E.

    2015-01-01

    Black mulberry fruit is processed to juice at significant scale in Turkey. The effect of industrial-scale juice production on black mulberry antioxidants was evaluated using samples collected from the main steps of processing; including the selection of fruits, washing, mechanical milling, mashing,

  7. 7 CFR 51.1179 - Method of juice extraction.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 2 2010-01-01 2010-01-01 false Method of juice extraction. 51.1179 Section 51.1179 Agriculture Regulations of the Department of Agriculture AGRICULTURAL MARKETING SERVICE (Standards... of Common Sweet Oranges (citrus Sinensis (l) Osbeck) § 51.1179 Method of juice extraction. The...

  8. An overview on the Brazilian orange juice production chain

    Directory of Open Access Journals (Sweden)

    Renato Marcio dos Santos

    2013-03-01

    Full Text Available Brazil is the world's largest producer of oranges and uses more than 70% of the harvested fruits in the production of juices. The amount of processed orange is growing about 10% per year, confirming the trend of the Brazilian citrus for juice production. This research aimed to investigate the Brazilian orange juice production chain from 2005 to 2009. Data from the amount of frozen juice produced and exported, international price of orange juice, and intermediate transactions were assessed in order to make possible selection of all interveners involved in the chain. The study using the Social Network Analysis (SNA showed that the densest relationships in the network are from exporters to importers and from orange growers to the orange processing industry. No difference was found in the values of the network geodesic distance or the clustering coefficients from 2005 to 2009. The degree of centrality increased steadily throughout the years indicating that the processing industry attempts to minimize the risks by centralizing the actions. A decrease in export of orange juice from 2007 (2.07 10(6 t to 2008 (2.05 10(6 t was found, probably due to the world's financial crisis with recovery in 2009. Since 2004, there has been an increase of nearly 10% per year in the market preference of concentrate juice (OFCJ when compared to the "not from concentrated" juice (NFC. Nowadays the NFC market represents nearly 50% of all Brazilian export which impacted in the logistic distribution and transportation issues.

  9. The mechanism of cloud loss phenomena in orange juice

    NARCIS (Netherlands)

    Krop, J.J.P.

    1974-01-01

    The importance of many factors for the cloud stability of orange juice was investigated. By the determination of methanol, cloud loss of orange juice could be ascribed directly to the action of pectin esterase. However, clarification only occurs if calcium ions are available to precipitate the low

  10. Comparative proteomic analysis of human pancreatic juice : Methodological study

    NARCIS (Netherlands)

    Zhou, Lu; Lu, ZhaoHui; Yang, AiMing; Deng, RuiXue; Mai, CanRong; Sang, XinTing; Faber, Klaas Nico; Lu, XingHua

    2007-01-01

    Pancreatic cancer is the most lethal of all the common malignancies. Markers for early detection of this disease are urgently needed. Here, we optimized and applied a proteome analysis of human pancreatic juice to identify biomarkers for pancreatic cancer. Pancreatic juice samples, devoid of blood o

  11. The Chemical and Educational Appeal of the Orange Juice Clock.

    Science.gov (United States)

    Kelter, Paul B.; And Others

    1996-01-01

    Describes the recent history, chemistry, and educational uses of the Orange Juice Clock demonstration in which a galvanic cell is made from the combination of a magnesium strip, a copper strip, and juice in a beaker. Discusses the chemistry basics, extensions for more advanced students, questions for student/teacher workshop participants, and…

  12. Comparative proteomic analysis of human pancreatic juice : Methodological study

    NARCIS (Netherlands)

    Zhou, Lu; Lu, ZhaoHui; Yang, AiMing; Deng, RuiXue; Mai, CanRong; Sang, XinTing; Faber, Klaas Nico; Lu, XingHua

    2007-01-01

    Pancreatic cancer is the most lethal of all the common malignancies. Markers for early detection of this disease are urgently needed. Here, we optimized and applied a proteome analysis of human pancreatic juice to identify biomarkers for pancreatic cancer. Pancreatic juice samples, devoid of blood o

  13. Comparative proteomic analysis of human pancreatic juice: Methodological study

    NARCIS (Netherlands)

    Zhou, Lu; Lu, Z.H.; Yang, A.M.; Deng, R.X.; Mai, C.R.; Sang, X.T.; Faber, Klaas Nico; Lu, X.H.

    2007-01-01

    Pancreatic cancer is the most lethal of all the common malignancies. Markers for early detection of this disease are urgently needed. Here, we optimized and applied a proteome analysis of human pancreatic juice to identify biomarkers for pancreatic cancer. Pancreatic juice samples, devoid of blood o

  14. Storage test on apple juice after ultrasound treatment

    Directory of Open Access Journals (Sweden)

    Filomena Montemurro

    2014-03-01

    Full Text Available Apple juice, for its sensory and nutritional qualities, is consumed by people of all ages. Apples are an excellent source of several phenolic compounds and the presence of polyphenols is recognized for their health promoting antioxidant properties. Thermal pasteurization of fruit juices is the conventional method used for their preservation. Therefore, this constitutes the most extensively available methods for the inactivation of microorganisms in fruit juices but it causes side effects on their flavour and nutritional quality. Consumers tend to prefer recently extracted juices with fresh taste and minimal flavor or vitamin losses. To meet consumers’ demand, among the novel technologies that involve non-thermal processes, power ultrasound have been investigated as an alternative to conventional heat treatments. Objective of this study was to evaluate the effectiveness of the use of ultrasound in an attempt to maintain the organoleptic characteristics typical of a natural apple juice. In particular, it was evaluated the action on the microflora residing and shelf life of the product through microbiological and sensory analyses. Juice treated with ultrasound highlighted a reduction of aerobic mesophilic counts and psychrophilic bacteria respectively about 3 and 5 log CFU/mL and an enhanced yeast growth. The general opinion expressed by the panelist was in favour of the sonicated juice. This preliminary study showed that non-thermal methods such as power ultrasound technology may give new opportunities to develop fresh-like apple juice.

  15. Storage Test on Apple Juice After Ultrasound Treatment

    Science.gov (United States)

    Fasolato, Luca; Balzan, Stefania; De Nardi, Roberta; Marchesini, Giorgio; Cardazzo, Barbara; Novelli, Enrico

    2014-01-01

    Apple juice, for its sensory and nutritional qualities, is consumed by people of all ages. Apples are an excellent source of several phenolic compounds and the presence of polyphenols is recognized for their health promoting antioxidant properties. Thermal pasteurization of fruit juices is the conventional method used for their preservation. Therefore, this constitutes the most extensively available methods for the inactivation of microorganisms in fruit juices but it causes side effects on their flavour and nutritional quality. Consumers tend to prefer recently extracted juices with fresh taste and minimal flavor or vitamin losses. To meet consumers’ demand, among the novel technologies that involve non-thermal processes, power ultrasound have been investigated as an alternative to conventional heat treatments. Objective of this study was to evaluate the effectiveness of the use of ultrasound in an attempt to maintain the organoleptic characteristics typical of a natural apple juice. In particular, it was evaluated the action on the microflora residing and shelf life of the product through microbiological and sensory analyses. Juice treated with ultrasound highlighted a reduction of aerobic mesophilic counts and psychrophilic bacteria respectively about 3 and 5 log CFU/mL and an enhanced yeast growth. The general opinion expressed by the panelist was in favour of the sonicated juice. This preliminary study showed that non-thermal methods such as power ultrasound technology may give new opportunities to develop fresh-like apple juice. PMID:27800306

  16. Changes in polyphenol content during production of grape juice concentrate

    NARCIS (Netherlands)

    Capanoglu, E.; Vos, de R.C.H.; Hall, R.D.; Boyacioglu, D.; Beekwilder, M.J.

    2013-01-01

    The production of grape juice concentrate on an industrial scale was evaluated and samples from the main steps of processing have been collected and analyzed. The sampling steps included the selection and washing of grapes (Nevsehir Patlak variety), pressing in order to obtain the juice separate

  17. The Chemical and Educational Appeal of the Orange Juice Clock.

    Science.gov (United States)

    Kelter, Paul B.; And Others

    1996-01-01

    Describes the recent history, chemistry, and educational uses of the Orange Juice Clock demonstration in which a galvanic cell is made from the combination of a magnesium strip, a copper strip, and juice in a beaker. Discusses the chemistry basics, extensions for more advanced students, questions for student/teacher workshop participants, and…

  18. Stabilization of anthocyanins in blackberry juice by glutathione fortification.

    Science.gov (United States)

    Stebbins, Nathan B; Howard, Luke R; Prior, Ronald L; Brownmiller, Cindi; Mauromoustakos, Andy

    2017-09-06

    Blackberry anthocyanins provide attractive color and antioxidant activity. However, anthocyanins degrade during juice processing and storage, so maintaining high anthocyanin concentrations in berry juices may lead to greater antioxidant and health benefits for the consumer. This study evaluated potential additives to stabilize anthocyanins during blackberry juice storage. The anthocyanin stabilizing agents used were: glutathione, galacturonic acid, diethylenetriaminepentaacetic acid and tannic acid, which were added at a level of 500 mg L(-1). Juice anthocyanin, flavonol, and ellagitannin content and percent polymeric color were measured over five weeks of accelerated storage at 30 °C. Glutathione had the greatest protective effect on total anthocyanins and polymeric color. Therefore a second study was performed with glutathione in combination with lipoic and ascorbic acids in an effort to use antioxidant recycling to achieve a synergistic effect. However, the antioxidant recycling system had no protective effect relative to glutathione alone. Glutathione appears to be a promising blackberry juice additive to protect against anthocyanin degradation during storage.

  19. Choice probability for apple juice based on novel processing techniques

    DEFF Research Database (Denmark)

    Olsen, Nina Veflen; Menichelli, E.; Grunert, Klaus G.

    2011-01-01

    and pulsed electric field (PEF) juice are compared with their probability of choice for pasteurized juice and freshly produced apple juice, and consumer choices are tried explained by values and consequences generated from a MEC study. The study support, at least partly, that means-end chain structures’ have......, within the core of academic consumer research, MEC has been almost ignored. One plausible explanation for this lack of interest may be that studies linking MEC data to choice have been few. In this study, we are to investigate how values and consequences generated from a previous MEC study structure can...... be linked to likelihood of choice. Hypotheses about European consumers’ likelihood of choice for novel processed juice are stated and tested in a rating based conjoint study in Norway, Denmark, Hungary and Slovakia. In the study, consumers probability of choice for high pressure processed (HPP) juice...

  20. Colour, phenolic content and antioxidant activity of grape juice

    Directory of Open Access Journals (Sweden)

    Vívian Maria Burin

    2010-12-01

    Full Text Available Viticultural practices in the State of Santa Catarina, Brazil, have shown economic growth, with the production of grapes used to produce wines and grape juice. Grapes are rich in phenolic compounds which have drawn attention not only because of their important role in the development of products derived from grapes, but also for their potential beneficial health effects. The objective of this study was to evaluate commercial, organic and homemade grape juices produced in Santa Catarina. Grape juices were analyzed for total phenolic content, colour, and antioxidant activity. The commercial juices had the highest average values for total monomeric anthocyanins and total phenolics. There was a strong positive correlation (R = 0.9566 between the antioxidant activity and total phenolic content for the commercial juice. In addition, the Principle Components Analysis showed a strong positive correlation between the red colour and total monomeric anthocyanins. However, the total monomeric anthocyanis and polymeric anthocyanins showed a negative correlation.

  1. CFDP Configuration: Enclid and Juice Scenarios

    Science.gov (United States)

    Valverde, Alberto; Taylor, Chris; Montesinos, Juan Antonio; Maiorano, Elena; Colombo, Cyril; Erd, Christian; Magistrati, Giorgio

    2014-08-01

    This paper presents the work done within the ESA ESTEC Data Systems Division, targeting the implementation of CFDP in future ESA Science Missions. EUCLID and JUICE currently include CCSDS File Delivery Protocol (CFDP) as baseline for payload data transfer to ground. The two missions have completely different characteristics, although both present quite demanding scenarios. Using the communication link characteristics as an input, some simulations have been performed to optimize the CFDP configuration and get some preliminary figures on the retransmission overhead, payload data bandwidth and number of parallel transactions needed to maintain full bandwidth utilization. The paper provides some guidelines on CFDP configuration and usage that can be useful in future CFDP implementations.

  2. Nitrile versus Latex for Glove Juice Sampling.

    Science.gov (United States)

    Landers, Timothy F; Dent, Anthony

    2014-01-01

    The objective of this study was to explore the utility of nitrile gloves as a replacement for latex surgical gloves in recovering bacteria from the hands. Two types of nitrile gloves were compared to latex gloves using the parallel streak method. Streaks of Klebsiella pneumoniae and Staphylococcus aureus were made on tryptic soy agar plates, and the zones of inhibition were measured around pieces of glove material placed on the plates. Latex gloves produced a mean zone of inhibition of 0.28 mm, compared to 0.002 mm for nitrile gloves (platex in glove juice sampling methods, since nitrile avoids the risk of latex exposure.

  3. Juice clarification by protease and pectinase treatments indicates new roles of pectin and protein in cherry juice turbidity

    DEFF Research Database (Denmark)

    Meyer, Anne S.; Zeuner, Birgitte; Pinelo-Jiménez, Manuel

    2010-01-01

    Industrial juice clarification is accomplished by a combination of enzymatic depectinization, gelatin–silica sol, and/or bentonite treatment. The gelatin–silica sol treatment step is particularly slow, mischievous, and requires comprehensive downstream processing to obtain clarified juice. In thi...

  4. Removal of fluoride from drinking water using aluminum hydroxide coated activated carbon prepared from bark of Morinda tinctoria

    Science.gov (United States)

    Amalraj, Augustine; Pius, Anitha

    2016-10-01

    The aim of this study is to design and develop a novel cost effective method for fluoride removal, applicable to rural areas of developing countries. Adsorption is widely considered as one of the appropriate technologies for water defluoridation. This study investigates the feasibility of using low-cost biomass based activated carbon from the bark of Morinda tinctoria coated with aluminum hydroxide (AHAC) for water defluoridation, at neutral pH range. Characterization of AHAC was done through IR, SEM with EDAX studies before and after fluoride treatment. The fluoride adsorption capacity of AHAC as a function of contact time, pH and initial fluoride concentration was investigated. The role of co-existing interfering ions also was studied. The isotherm and kinetic models were used to understand the nature of the fluoride adsorption onto AHAC. Freundlich isotherm and intra-particle diffusion were the best-fitting models for the adsorption of fluoride on AHAC. Fluoride adsorption kinetics well fitted with pseudo-second order model. The results showed excellent fluoride adsorption capacity was found to be 26.03 mg g-1 at neutral pH.

  5. Synthesis of eco-friendly silver nanoparticles from Morinda tinctoria leaf extract and its larvicidal activity against Culex quinquefasciatus.

    Science.gov (United States)

    Kumar, K Ramesh; Nattuthurai, N; Gopinath, Ponraj; Mariappan, Tirupathi

    2015-02-01

    Mosquitoes are the major vector for the transmission of malaria, dengue fever, yellow fever, filariasis, chikungunya and Japanese encephalitis, and they accounted for global mortality and morbidity with increased resistance to common insecticides. The aim of this study was to investigate the larvicidal potential of the acetone leaf extracts of Morinda tinctoria and synthesized silver nanoparticles against third instar larvae of Culex quinquefasciatus Say (Diptera: Culicidae). Nanoparticles are being used in many commercial applications. It was found that aqueous silver ions can be reduced by aqueous extract of plant parts to generate extremely stable silver nanoparticles in water. Synthesized AgNPs were characterized by ultraviolet-visible (UV-vis) spectroscopy, Atomic force microscopy (AFM) and Fourier transform infrared spectroscopy (FT-IR) analysis. The synthesized silver nanoparticles have also been tested against the third instar larvae of C. quinquefasciatus. The leaf extract and the AgNPs high mortality values were 50 % lethal concentration (LC50) = 8.088 and 1.442 ppm against C. quinquefasciatus, respectively. The results recorded from ultraviolet-visible spectroscopy, atomic force microscopy and Fourier transform infrared spectroscopy support the biosynthesis and characterization of silver nanoparticles. These results suggest that the leaf extract of M. tinctoria and synthesis of AgNPs have the potential to be used as an ideal eco-friendly approach for the control of C. quinquefasciatus. By this approach, it is suggestive that this rapid synthesis of nanoparticles would be proper for developing a biological process for mosquito control.

  6. EFFECT OF DICHLOROMETHANE-ETHANOL EXTRACT OF MORINDA MORINDOIDES (BACKER MILNE-REDHEAD (RUBIACEAE (ETDE ON RABBIT CAROTID BLOOD PRESSURE

    Directory of Open Access Journals (Sweden)

    Boga Gogo Lucien

    2013-08-01

    Full Text Available Morinda morindoides (Backer Milne-Redhead (Rubiaceae is used by the people of west and central Africa for the treatment of diarrhea. The dichloromethane/ethanol extract of M. morindoides (Back (ETDE known to be used orally, will be in direct contact with the nobles organs. This study is conduced to see if this extract has effects on the body more precisely on blood pressure. ETDE injected intravenously (10.40 mg/kg b.w to 31.19 mg/kg b.w provoked a decrease in the arterial blood pressure (hypotension in a dose-dependent manner (ED50 = 7.08 mg/kg b.w. ETDE at 41.58 mg/kg b.w induce a maximum and irreversible hypotension which leads to the death of the animal. The effects induced by ETDE were inhibited in the presence of atropine at a concentration of 4.46 ×10-4 mg/kg b.w. Our observations, regarding the effects of ETDE on the high blood pressure initiated by adrenaline, showed that the hypertensive effects induced by adrenalin were totally inhibited by ETDE. ETDE induced a dose-dependent hypotension and reversible and his antihypertensive effect could militate for its use in the treatment of hypertension.

  7. Antioxidant and cytotoxic effects of hexane extract ofMorinda pubescens leaves in human liver cancer cell line

    Institute of Scientific and Technical Information of China (English)

    Jaya Kumar D; Jaya Santhi R

    2012-01-01

    ABSTRACT Objective:To evaluate the antioxidant and cytotoxic effects of hexane extract ofMorinda pubescens leaves and to find the primary bioactive compound responsible for antioxidant and cytotoxic activities.Methods: The individual compounds were isolated using column chromatography and were characterized by spectroscopic techniques. The antioxidant activity was evaluated for all individual isolated compounds byDPPH method usingL-Ascorbic acid as standard and cytotoxicity was assessed for the extract and the hyoscyamine byMTT assay, caspase test andRT-PCR study.Results:The antioxidant activity of the isolated compounds and the extract increased as the concentration increased. One of the isolated compound hyoscyamine showed the high antioxidant activity. The extract and the hyoscyamine dose-dependently decreased the cell viability in HepG2 cells. Hyoscyamine induced caspase-3mediated apoptosis. Up regulation of p53 gene expression provides cue for apoptotic activity of hyoscyamine. Conclusions: The results indicate that hexane extract possessed potent antioxidant and cytotoxic activity and hyoscyamine is the principal bioactive compound in hexane extract.

  8. Coca-Cola's Acquisition Over Huiyuan Juice

    Institute of Scientific and Technical Information of China (English)

    Jin Sun

    2009-01-01

    @@ China's Anti-Monopoly Law,the foundation stone of the market economy,started to be implemented since August 1st 2008.After half a year of investigation,.Coca Cola's acquisition of Huiyuan Juice was put to a stop by Ministry of Commerce on March 18th 2009.It became the first abortive case since the implementation of the Anti-Monopoly Law and caused strong repercussions home and abroad.While the nationalists applauded for the decision,overseas media generally took a skeptical attitude,considering it as a case of trade protectionism.This phenomenon requires a rational interpretation and guidance for the domestic and international media.On the one hand,acquisition by foreign capital may bring advanced technology and management experience and promote economic development;on the other hand,it may lead to the monopoly of relevant market,hamper competition and cause some domestic brands to disappear.We should look at Coca Cola's acquisition of Huiyuan Juice from two aspects.

  9. Comparison of the polyphenolic composition and antioxidant activity of European commercial fruit juices.

    Science.gov (United States)

    Borges, Gina; Mullen, William; Crozier, Alan

    2010-10-01

    Thirty six commercial European fruit juices were tested to ascertain their antioxidant capacity and polyphenolic composition. Six of the products were labelled 100% pomegranate juice, the others included 20 brands of diluted pomegranate juice or pomegranate blended with other fruit juices and 10 different non-pomegranate fruit juices. The antioxidant capacity of all the juices was determined while anthocyanin, ellagitannin and ellagic acid profiles of the 26 pomegranate juices and pomegranate juice blends were obtained using HPLC-PDA-MS(2). Additional analysis was conducted on seven of the juices using HPLC with an on-line antioxidant detection system. Three of the "pure" pomegranate juices had the highest ellagitannin content and the highest antioxidant capacity. Only one of these three juices was rich in anthocyanins. The other "pure juices" had differences in their HPLC "pomegranate" fingerprint and also had a lower antioxidant capacity, in some cases lower than that of some of the blended juices. Vitamin C rather than phenolic compounds was the major contributor to the antioxidant capacity for some of the juices. Statistical analysis of both the antioxidant assay and the HPLC on-line antioxidant data demonstrated that the ellagitannins were the major antioxidants in the pomegranate juices. The complexity of the polyphenolic profile of pomegranates necessitates the use of HPLC-PDA-MS(2) for a thorough evaluation of juice composition and authenticity.

  10. 21 CFR 146.148 - Reduced acid frozen concentrated orange juice.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 2 2010-04-01 2010-04-01 false Reduced acid frozen concentrated orange juice. 146... SERVICES (CONTINUED) FOOD FOR HUMAN CONSUMPTION CANNED FRUIT JUICES Requirements for Specific Standardized Canned Fruit Juices and Beverages § 146.148 Reduced acid frozen concentrated orange juice. (a) Reduced...

  11. 27 CFR 24.180 - Use of concentrated and unconcentrated fruit juice.

    Science.gov (United States)

    2010-04-01

    ... unconcentrated fruit juice. 24.180 Section 24.180 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX... concentrated and unconcentrated fruit juice. Concentrated fruit juice reduced with water to its original..., and unconcentrated fruit juice reduced with water to not less than 22 degrees Brix, is considered...

  12. 19 CFR 151.91 - Brix values of unconcentrated natural fruit juices.

    Science.gov (United States)

    2010-04-01

    ... 19 Customs Duties 2 2010-04-01 2010-04-01 false Brix values of unconcentrated natural fruit juices... Juices § 151.91 Brix values of unconcentrated natural fruit juices. The following values have been determined to be the average Brix values of unconcentrated natural fruit juices in the trade and commerce...

  13. Impact of Frozen Storage on the Anthocyanin and Polyphenol Contents of American Elderberry Fruit Juice.

    Science.gov (United States)

    Johnson, Mitch C; Thomas, Andrew L; Greenlief, C Michael

    2015-06-17

    The effects of frozen storage on the anthocyanin and polyphenol content of elderberry fruit juice are investigated. Juice from three genotypes of American elderberry (Adams II, Bob Gordon, and Wyldewood) was screened for total phenolic (TP) and total monomeric anthocyanin (TMA) contents with spectrophotometric methods. The individual anthocyanin content (IAC) of the juice was tested by coupling solid phase extraction with ultraperformance liquid chromatography-tandem mass spectrometry. Juice samples were tested initially upon harvest and then again after 3, 6, and 9 months of frozen storage. Juice from the three different genotypes had significantly different TP, TMA, and IAC profiles initially (p juice from different genotypes were significantly affected (p fruit juice.

  14. Fenton Discoloration of Ultrasonicated Purple Cactus Pear Juice.

    Science.gov (United States)

    Reyes-Hernández, Isidro; Cruz-Cansino, Nelly Del S; Santander-Martínez, Ingrid Renata; Alanís-García, Ernesto; Delgado-Olivares, Luis; Ramírez-Moreno, Esther; Ariza-Ortega, José A; Omaña-Covarrubias, Ariana; Torres-Valencia, Jesús Martín; Manríquez-Torres, José de Jesús

    2017-08-15

    The aim of this study was to evaluate the stability of color, betaxanthin, and betacyanin pigments in the presence of Cu(II)-dependent hydroxyl radicals (HO•) from ultrasonicated purple cactus pear juice at amplitudes of 40%, 60%, and 80%, in comparison to untreated sample. L* parameter of juice treated at 40% and 80% amplitude for 25 and 15 min, respectively (11.3 and 9.3, respectively), were significantly higher compared to the control; b* and hue parameters of juice treated at 80%, 25 min showed values of 1.7 and 0.1, respectively. Color differences (ΔE) were lower (juices treated at high amplitude (80%) and short times (3-5 min). Juice treated at 40% 15 min, 60% 25 min, 80% 15 and 25 min presented high values of betacyanins (281.7 mg·L(-1), 255.9 mg·L(-1), 294.4 mg·L(-1), and 276.7 mg·L(-1), respectively). Betaxanthin values were higher in the juices treated at 40% 5 min and 80% 15 and 25 min (154.2 mg·L(-1), 135.2 mg·L(-1), and 128.5 mg·L(-1), respectively). Purple cactus pear juice exhibited significant chelating activity of copper ions and great stability when exposed to HO•.

  15. Isotope analysis (δ13C of pulpy whole apple juice

    Directory of Open Access Journals (Sweden)

    Ricardo Figueira

    2011-09-01

    Full Text Available The objectives of this study were to develop the method of isotope analysis to quantify the carbon of C3 photosynthetic cycle in pulpy whole apple juice and to measure the legal limits based on Brazilian legislation in order to identify the beverages that do not conform to the Ministry of Agriculture, Livestock and Food Supply (MAPA. This beverage was produced in a laboratory according to the Brazilian law. Pulpy juices adulterated by the addition of sugarcane were also produced. The isotope analyses measured the relative isotope enrichment of the juices, their pulpy fractions (internal standard and purified sugar. From those results, the quantity of C3 source was estimated by means of the isotope dilution equation. To determine the existence of adulteration in commercial juices, it was necessary to create a legal limit according to the Brazilian law. Three brands of commercial juices were analyzed. One was classified as adulterated. The legal limit enabled to clearly identify the juice that was not in conformity with the Brazilian law. The methodology developed proved efficient for quantifying the carbon of C3 origin in commercial pulpy apple juices.

  16. Recovery of alicyclobacillus from inhibitory fruit juice concentrates.

    Science.gov (United States)

    McNamara, Christopher J; Wiebe, Deborah; Gomez, Margarita

    2011-08-01

    Growth of Alicyclobacillus in low-pH fruit juices may result in off-odors and off-flavors due to the production of compounds such as guaiacol (2-methoxy phenol). An important step in preventing Alicyclobacillus contamination of fruit juices is the screening of incoming ingredients. Many fruit juice concentrates contain compounds that inhibit Alicyclobacillus growth, but beverages produced from the concentrates may not contain sufficient amounts of the active component to prevent spoilage. Therefore, accurate screening of juice concentrates is essential to prevent false-negative test results and product spoilage. The objective of this study was to evaluate isolation methods for detection of Alicyclobacillus in inhibitory juice concentrates. Recovery of Alicyclobacillus spores from inoculated and naturally contaminated concentrates was compared by using pour plate, spread plate, and filtration methods. Pour plates consistently recovered the lowest number of spores from inoculated concentrates. Spread plating was the most effective method used to recover spores from inoculated apple and pomegranate juice concentrates, while filtration resulted in the highest recovery from cranberry concentrate. When tested on naturally contaminated concentrates, the pour plate method failed to detect Alicyclobacillus in many samples. Filtration was much more effective. The filtration method increased the likelihood of detecting Alicyclobacillus contamination of fruit juice concentrates containing inhibitory compounds.

  17. Uses of miscanthus press juice within a green biorefinery platform.

    Science.gov (United States)

    Boakye-Boaten, Nana Abayie; Xiu, Shuangning; Shahbazi, Abolghasem; Wang, Lijun; Li, Rui; Schimmel, Keith

    2016-05-01

    This study assesses some uses of nutrient-rich juice mechanically extracted from freshly harvested Miscanthus x giganteus (MxG) as part of a green biorefinery system. The juice was used for culturing Saccharomyces cerevisiae and lactic acid bacteria. MxG juice was further used as substrate for fermentation to produce lactic acid using Lactobacillus brevis and Lactobacillus plantarum. The results show that MxG juice was a highly nutritious source for the cultivation of bacteria. Higher concentrations of MxG juice used as culture media, resulted in higher cell growth both aerobically and anaerobically. The highest ethanol yield of 70% theoretical and concentration of 0.75g/100ml were obtained from S. cerevisiae cultivated with 90% (v/v) MxG juice media and used for miscanthus solid fraction fermentation. 11.91g/L of lactic acid was also successfully produced from MxG juice through SSF. Copyright © 2016 Elsevier Ltd. All rights reserved.

  18. Phenolics content and antioxidant capacity of commercial red fruit juices

    Directory of Open Access Journals (Sweden)

    Mitić Milan N.

    2011-01-01

    Full Text Available The content of phenolics: total phenols (TP, flavonoids (TF, anthocyanins (TA and hydroxicinnamic acid as well as the total antioxidant capacity (TAC in nine commercial red fruit juices (sour cherry, black currant, red grape produced in Serbia were evaluated. The total compounds content was measured by spectrophotometric methods, TAC was determined using DPPH assays, and individual anthocyanins and hydroxycinnamic acids was determined using HPLC-DAD methods. Among the examined fruit juices, the black currant juices contained the highest amounts of all groups of the phenolics and exhibited strong antioxidant capacity. The amount of anthocyanins determined by HPLC method ranged from 92.36 to 512.73 mg/L in red grape and black currant juices, respectively. The anthocyanins present in the investigated red fruit juices were derivatives of cyanidin, delphinidin, petunidin, peonidin and malvidin. The predominant phenolic acid was neoclorogenic acid in sour cherry, caffeic acid in black currant, and p-coumaric acid in black grape juices. Generally, the red fruit juices produced in the Serbia are a rich source of the phenolic, which show evident antioxidant capacity.

  19. Socio-economic status and fruit juice consumption in Canada.

    Science.gov (United States)

    Shupler, Matthew; Raine, Kim D

    2017-06-16

    The role of socio-economic status (SES) in fruit juice and fruit drink consumption is not well understood in a Canadian context. This study examines the relationship between SES and Canadian fruit juice and fruit drink consumption. The Canadian Community Health Survey (2011-2012), a cross-sectional survey that employs multistage cluster sampling, provided relevant data for a sample of 103 125 Canadians, aged 12 and older, living in the 10 provinces. Household income level decile, ranked at the health region level, was used as a surrogate measure of SES. Fruit juice and fruit drink consumption data were collected via self-report in telephone/in-person interviews. Multivariable gamma regression was used to model the relationship between SES and frequency of fruit juice and fruit drink consumption, adjusting for age, sex, diabetes status, daily fruit and vegetable consumption, education level, racial identity and physical activity. A negative relationship was found, with a decreasing daily rate of fruit juice and fruit drink consumption associated with increasing SES. In the adjusted model, Canadians in the lowest SES category consumed fruit juice and fruit drinks at an average daily rate 1.18 times (95% CI: 1.14-1.23) that of Canadians in the highest SES category. The negative association between health region-adjusted SES and fruit juice and fruit drink consumption highlights the potentially important role of socio-economic factors at a local level. Canadian policy that aims to lower fruit juice and fruit drink consumption, and thus sugar intake, should target financial avenues (such as making fruit juice less financially attractive by lowering the cost of whole fruit and vegetables) in addition to communicating health benefits.

  20. Effects of Juice Matrix and Pasteurization on Stability of Black Currant Anthocyanins during Storage.

    Science.gov (United States)

    Dobson, Gary; McDougall, Gordon J; Stewart, Derek; Cubero, Miguel Ángel; Karjalainen, Reijo O

    2017-01-01

    The effects of juice matrix and pasteurization on the stability of total phenols and especially total and individual anthocyanins were examined in black currant (BC) juice and mixtures with apple, persimmon, and peach juices at 4 °C and 20 °C. Total phenol content decreased in all juices at both temperatures but there was a trend to lower levels in unpasteurized over pasteurized juices. Differences in the decline of total anthocyanins between pasteurized and unpasteurized juices varied according to the juice type and the storage temperature. At 4 °C storage, anthocyanins declined in all juices according to pseudo 1st-order kinetics and there were only small differences in the rates between pasteurized and unpasteurized juices. However, at 20 °C, although pasteurized and unpasteurized BC juices and pasteurized mixed juices followed pseudo 1st-order kinetics, there was a different pattern in unpasteurized mixed juices; a rapid initial decline was followed by a slowing down. The effect of the added juice on anthocyanin decline was also different at either temperature. At 4 °C, the anthocyanins decreased faster in mixed juices than BC juice alone, but at 20 °C, at least in pasteurized mixed juices, the decline was similar or even slower than in BC juice; there were only small differences among the 3 mixed juices. At 20 °C, in pasteurized and unpasteurized BC juices, the rate of decrease was essentially the same for all 4 individual anthocyanins but in the mixed juices the 2 glucosides decreased significantly faster than the 2 rutinosides. © 2016 Institute of Food Technologists®.

  1. In Vivo Antioxidant Activity of Deacetylasperulosidic Acid in Noni

    Directory of Open Access Journals (Sweden)

    De-Lu Ma

    2013-01-01

    Full Text Available Deacetylasperulosidic acid (DAA is a major phytochemical constituent of Morinda citrifolia (noni fruit. Noni juice has demonstrated antioxidant activity in vivo and in human trials. To evaluate the role of DAA in this antioxidant activity, Wistar rats were fed 0 (control group, 15, 30, or 60 mg/kg body weight per day for 7 days. Afterwards, serum malondialdehyde concentration and superoxide dismutase and glutathione peroxidase activities were measured and compared among groups. A dose-dependent reduction in malondialdehyde was evident as well as a dose-dependent increase in superoxide dismutase activity. DAA ingestion did not influence serum glutathione peroxidase activity. These results suggest that DAA contributes to the antioxidant activity of noni juice by increasing superoxide dismutase activity. The fact that malondialdehyde concentrations declined with increased DAA dose, despite the lack of glutathione peroxidase-inducing activity, suggests that DAA may also increase catalase activity. It has been previously reported that noni juice increases catalase activity in vivo but additional research is required to confirm the effect of DAA on catalase. Even so, the current findings do explain a possible mechanism of action for the antioxidant properties of noni juice that have been observed in human clinical trials.

  2. Crossflow microfiltration of sugarcane juice: effects of processing conditions and juice quality

    Directory of Open Access Journals (Sweden)

    Katia Rezzadori

    2014-03-01

    Full Text Available Sugarcane juice with passion fruit pulp was clarified using microfiltration under different T (temperature, P (pressure, and V (tangential velocity. The effects of these processing parameters were evaluated applying a rotational central composite experimental design (RCCD and response surface methodology (RSM. The tests were performed at a filtration pilot plant using a polyamide hollow-fiber membrane with an average pore diameter of 0.4 µm and filtration area of 0.723 m². In addition, the resistances to the permeate flux during the microfiltration were investigated according to the series resistance. The final permeate flux ranged from 7.05 to 17.84 L·h- 1·m- 2. There was a rapid decline in flux (50% in the initial stages of microfiltration. T and V were the major variables responsible for the flux increase. The concentration polarization showed the greatest influence on the flux decline, and highest values for the flux decline rate (λ were found when low pressures were used. In the clarified juice there was a reduction in the contents of total solids, proteins, vitamin C, and acidity, while the soluble solids, pH, and ash contents did not change. Finally, membrane process could produce high quality filtered sugarcane juice with substantial flux and increased luminosity improving organoleptical properties.

  3. Use of Moringa oleífera Lamarck leaf extract as sugarcane juice clarifier: effects on clarifed juice and sugar

    Directory of Open Access Journals (Sweden)

    Gustavo Henrique Gravatim Costa

    2014-03-01

    Full Text Available The objective of this study was to evaluate the effect of Moringa oleifera Lam. leaf extract on the sedimentation of impurities in the treatment of sugarcane juice and the effects on sugar quality and on the clarified juice. The experimental design used was a 4x2 factorial arrangement with four replications. The main treatments performed included the extracted original sugarcane juice, the synthetic polyelectrolyte (Flomex 9076, the leaf extract, and a control. The secondary treatments consisted of the sugarcane varieties RB92579 and RB867515. The clarification process used was simple defecation, in which the flocculating agents and the juice, limed and heated, were poured simultaneously into a decanter. The microbiological and chemico-technological characteristics of the extracted and clarified juices were evaluated. The clarified juice was concentrated up to 60° Brix (syrup and subjected to boiling in a pilot pan using seeds to perform the graining: The sugar was recovered by centrifugation and analyzed for microbiological and chemico-technological characteristics. It was concluded that the use of the Moringa oleifera Lam. leaves extract resulted in a better quality of clarified juice and sugar.

  4. Application of membrane separation in fruit and vegetable juice processing: a review.

    Science.gov (United States)

    Ilame, Susmit A; Satyavir, V Singh

    2015-01-01

    Fruit and vegetable juices are used due to convenience. The juices are rich in various minerals, vitamins, and other nutrients. To process the juices and their clarification and/or concentration is required. The membranes are being used for these purposes. These processes are preferred over others because of high efficiency and low temperature. Membranes and their characteristics have been discussed in brief for knowing suitability of membranes for fruit and vegetable juices. Membrane separation is low temperature process in which the organoleptic quality of the juice is almost retained. In this review, different membrane separation methods including Microfiltration, Ultrafiltration, and Reverse osmosis for fruit juices reported in the literature are discussed. The major fruit and vegetable juices using membrane processes are including the Reverse osmosis studies for concentration of Orange juice, Carrot juice, and Grape juice are discusses. The Microfiltration and Ultrafiltration are used for clarification of juices of mosambi juice, apple juice, pineapple juice, and kiwifruit juice. The various optimized parameters in membranes studies are pH, TAA, TSS, and AIS. In this review, in addition to above the OD is also discussed, where the membranes are used.

  5. Statement on ‘toothkind’ juice drinks

    DEFF Research Database (Denmark)

    Tetens, Inge

    2011-01-01

    of tooth demineralisation, can be drawn on the basis of studies which do not measure directly net demineralisation but measure the potential for demineralisation of enamel, e.g. reduced pH on dental plaque. The Panel considers that reduction of plaque pH immediately (within about 10 min) following a single...... consumption of a beverage is an appropriate measure of the potential of beverages for demineralisation of dental enamel. „Toothkind‟ drinks have little or no potential for enamel demineralisation by this process, while typical sugar-containing non-alcoholic beverages do have the potential for demineralisation...... of dental enamel. However, the beneficial effect (reducing net tooth demineralisation) of replacing typical sugar-containing non-alcoholic beverages with „toothkind‟ juice drinks was only shown to occur at a frequency of consumption of typical sugar-containing non-alcoholic beverages of 7 times daily...

  6. Dynamic headspace analysis of fresh tomato juices.

    Science.gov (United States)

    Sucan, M K; Russell, G F

    2001-01-01

    The methods used to isolate volatile compounds for GC analyses can cause profound effects on the quantitative and qualitative composition of the injected sample, and exert a great influence in the resultant bioactivity of volatiles. Especially with plant tissues like tomatoes, the isolation of volatile constituents using classical methods may yield results which are not representative of the chemicals present in the natural material. Headspace sampling methods may be advantageous in capturing the same volatile compounds emitted from tomatoes that are detected by the human nose. This study utilized an extremely sensitive dynamic headspace sampling with thermal desorption method to determine volatile components of fresh tomato juices. The method proved very sensitive for the isolation of tomato volatiles and concentrations of flavor compounds were much greater than related literature studies.

  7. Chemical Composition of Selected Beetroot Juices in Relation to Beetroot Production System and Processing Technology

    Directory of Open Access Journals (Sweden)

    Renata KAZIMIERCZAK

    2016-12-01

    Full Text Available Market offer of vegetable juices in Europe is growing, and the vegetable species and processing technologies used become more diversified resulting in a large range of juice types. At the same time consumers look for natural and safe products with pro-health properties. The aim of this study was to evaluate the nutritional composition of selected juices based on beetroots coming from different agricultural systems and processed according to different technologies. Research material consisted of conventional and organic juices marketed in Poland, in that pure pressed juices, fermented juice and juices from concentrate, pure and combined with apple or lemon juice. The concentration of vitamin C, organic acids, total and reducing sugars, phenolic acids, flavonoids and betalains has been determined in the juices. All beetroot juices were abundant in betalains, with higher concentrations of these compounds found in pure beetroot juices when compared to juices combined with apple or lemon. Highest concentration of betanin-3-O-glucoside was found in pure conventional juice from concentrate, while highest betanidin content was found in pure organic fermented juice. Highest vitamin C contents were found in pressed organic juices combined with apple and lemon. All juices were abundant in polyphenols, mainly phenolic acids, with highest concentrations of these antioxidants found in conventional pure pressed juice. The study shows that beetroot juices, independently on the beetroot processing technology and agricultural production system, are very valuable products due to the presence of numerous bioactive compounds, especially betalains, in their composition. Therefore beetroot juice consumption should be promoted among consumers.

  8. Clarification Effects of Chitosan on Apple Fruit Juice

    Institute of Scientific and Technical Information of China (English)

    WANG Hong-fei; LI He-sheng; ZHANG Xiao-ping; HUANG Xiao-chun

    2003-01-01

    Chitosan is a good flocculant. The paper deals with the clarification of apple juice by means of chitosan. The results showed that the transmittance was over 97% and soluble solid content was stable basically, under the technological condition of chitosan of 0.5 - 1.2 g L-1 , temperature of 45 - 55℃ and pH 4.5. After the orthogonal trial, the optimum technological conditions of apple juice clarification by using chitosan were 0.3 g L-1 chitosan, 45℃C and pH 4.5. The research of the apple juice clarification with chitosan made a basis of the application of chitosan and provided the theoretical basis for the clarification of apple juice with chitosan.

  9. LIFE CYCLE DESIGN OF MILK AND JUICE PACKAGING

    Science.gov (United States)

    A life cycle design demonstration project was initiated between the U.S. Environmental Protection Agency, National Risk Management Research Laboratory, Dow Chemical Company, and the University of Michigan to investigate milk and juice packagie design. The primary objective of ...

  10. PREBIOTICS AS DRYING AIDS FOR SPRAY DRYING FRUIT JUICES

    Directory of Open Access Journals (Sweden)

    R. FREIXO

    2016-10-01

    Full Text Available Natural fruit juices are difficult to transform into powder because they are rich in sugars and acids with low glass transition temperatures, resulting in a sticky behaviour during spray drying. The present research work aimed to test the prebiotics maltodextrin and arabic gum as drying agents in order to be able to produce dehydrated powder from pineapple, strawberry and apple juices. 2 % of maltodextrin and an inlet air temperature of 130 °C are recommended to spray dry apple juice, reaching 60 % yield of powder with a water activity below 0.40. For strawberry juice, 2 % of this carrier is recommended and the inlet air temperature, 120 °C or 130 °C, did not seem relevant. For pineapple, neither the concentration of the carrier nor the inlet air temperature seemed to be relevant.

  11. Enhanced ethanol production from stalk juice of sweet sorghum by ...

    African Journals Online (AJOL)

    user

    2012-03-15

    Mar 15, 2012 ... ethanol production by a Saccharomyces cerevisiae strain because of the high content of sugar. ... Key words: Ethanol, sweet sorghum, stalk juice, medium ..... production from Kinnow mandarin (Citrus reticulata) waste via a.

  12. Spray drying of fruit and vegetable juices--a review.

    Science.gov (United States)

    Verma, Anjali; Singh, Satya Vir

    2015-01-01

    The main cause of spray drying is to increase the shelf life and easy handling of juices. In the present paper, the studies carried out so far on spray drying of various fruits and vegetables are reported. The major fruit juices dried are mango, banana, orange, guava, bayberry, watermelon, pineapple, etc. However, study on vegetable juices is limited. In spray drying, the major optimized parameters are inlet air temperature, relative humidity of air, outlet air temperature, and atomizer speed that are given for a particular study. The juices in spray drying require addition of drying agents that include matlodextrin, liquid glucose, etc. The drying agents are added to increase the glass transition temperature. Different approaches for spray dryer design have also been discussed in the present work.

  13. Orange proteomic fingerprinting: From fruit to commercial juices.

    Science.gov (United States)

    Lerma-García, María Jesús; D'Amato, Alfonsina; Simó-Alfonso, Ernesto F; Righetti, Pier Giorgio; Fasoli, Elisa

    2016-04-01

    Combinatorial peptide ligand library technology, coupled to mass spectrometry, has been applied to extensively map the proteome of orange pulp and peel and, via this fingerprinting, to detect its presence in commercial orange juices and drinks. The native and denaturing extraction protocols have captured 1109 orange proteins, as identified by LC-MS/MS. This proteomic map has been searched in an orange concentrate, from a Spanish juice manufacturer, as well as in commercial orange juices and soft drinks. The presence of numerous orange proteins in commercial juices has demonstrated the genuineness of these products, prepared by using orange fruits as original ingredients. However, the low number of identified proteins in sparkling beverages has suggested that they were prepared with scarce amounts of fruit extract, thus imparting lower quality to the final products. These findings not only increase the knowledge of the orange proteome but also present a reliable analytical method to assess quality and genuineness of commercial products.

  14. Development of functional beverage from wheat grass juice

    Directory of Open Access Journals (Sweden)

    Liana Claudia SALANTA

    2016-11-01

    Full Text Available The juice from wheat grass is called "green blood" and is an excellent detoxifying, facilitating the elimination of toxins and fats from body. In the form of fresh juice, it has high concentrations of chlorophyll, active enzymes, vitamins and other nutrients. The aim of this work was the development and characterization of a functional beverage from green wheat juice by adding apple and limes. The antioxidant capacity, vitamin C, polyphenols and flavonoids content were quantified by using spectrophotometry. The final product was pasteurized and evaluated by the content of bioactive compounds during storage at intervals of 7 and 14 days. During storage there were found slight decreases of the contents of bioactive compounds. The juice obtained has a sweet-sour taste, a unique flavor and a very pleasant smell. This product targets all categories of consumers and represents an ideal morning snack for those who are concerned about a healthy lifestyle.

  15. MICROBIOLOGICAL AND PHYSICAL PROPERTIES OF BEEF MARINATED WITH GARLIC JUICE

    Directory of Open Access Journals (Sweden)

    N. Nurwantoro

    2014-10-01

    Full Text Available The study was carried out to investigate the effect of marination, performed by soaking of beef ingarlic juice, on microbiological and physical properties. The study was committed to a completelyrandomized design, with 5 treatments, i.e.: T0 (unmarinated beef, as a control, T1, T2, T3, and T4 thatbeef were marinated in garlic juice for 5, 10, 15, and 20 minutes, respectively, at room temperature(25⁰C. Each treatment consisted of 4 replications. Examination upon experimental parameters wasconducted after marinated (and control beef was stored for 8 hours at room temperature. Total bacteria,total coliform and water holding capacity of beef were significantly (P<0.05 affected by marinationwith garlic juice. Conversely, cooking loss was not significantly affected (P>0.05 by the treatments. Asa conclusion, marination of beef with garlic juice could reduce total bacteria, total coliform, and waterholding capacity, but could not reduce cooking loss.

  16. Chemical and physicochemical characteristics changes during passion fruit juice processing

    Directory of Open Access Journals (Sweden)

    Aline Gurgel Fernandes

    2011-09-01

    Full Text Available Passion fruit is widely consumed due to its pleasant flavour and aroma acidity, and it is considered very important a source of minerals and vitamins. It is used in many products such as ice-cream, mousses and, especially, juices. However, the processing of passion fruit juice may modify the composition and biodisponibility of the bioactive compounds. Investigations of the effects of processing on nutritional components in tropical juices are scarce. Frequently, only losses of vitamin C are evaluated. The objective of this paper is to investigate how some operations of passion fruit juice processing (formulation/homogeneization/thermal treatment affect this product's chemical and physicochemical characteristics. The results showed that the chemical and physicochemical characteristics are little affected by the processing although a reduction in vitamin C contents and anthocyanin, large quantities of carotenoids was verified even after the pasteurization stage.

  17. LIFE CYCLE DESIGN OF MILK AND JUICE PACKAGING

    Science.gov (United States)

    A life cycle design demonstration project was initiated between the U.S. Environmental Protection Agency, National Risk Management Research Laboratory, Dow Chemical Company, and the University of Michigan to investigate milk and juice packagie design. The primary objective of ...

  18. Phytochemical, antimicrobial, and antioxidant activities of different citrus juice concentrates.

    Science.gov (United States)

    Oikeh, Ehigbai I; Omoregie, Ehimwenma S; Oviasogie, Faith E; Oriakhi, Kelly

    2016-01-01

    The search for new antimicrobial compounds is ongoing. Its importance cannot be overemphasized in an era of emerging resistant pathogenic organisms. This study therefore investigated the phytochemical composition and antioxidant and antimicrobial activities of different citrus juice concentrates. Fruit juices of Citrus tangerine (tangerine), Citrus paradisi (grape), Citrus limon (lemon), and Citrus aurantifolia (lime) were evaluated. Antimicrobial activities against five bacterial and three fungal strains were evaluated. The results revealed the presence of alkaloids, flavonoids, steroids, terpenoids, saponins, cardiac glycosides, and reducing sugars in all the juice concentrates. DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging capacities varied with tangerine and grape juices having better scavenging capacities than lemon and lime juices. Grape juice was observed to have a significantly higher (P < 0.05) ferric-reducing antioxidant potential (FRAP) value (364.2 ± 10.25 μmol/L Fe(II)/g of the extract) than the reference antioxidant, ascorbic acid (312.88 ± 5.61 μmol/L). Antimicrobial studies revealed differential antimicrobial activities against different microbial strains. Zones of inhibition ranging from 4 to 26 mm were observed for the antibacterial tests with 0-24 mm for antifungal test. Minimum inhibitory concentrations (MIC) and minimum bacteriostatic concentrations (MBC) for concentrates against bacterial strains ranged from 12.5 to 200 μg/mL. Lemon and lime juice concentrates had lower MIC and MBC values with orange and tangerine having the highest values. Minimum fungicidal concentrations ranged from 50 to 200 μg/mL. The results of this study suggest that these juice concentrates may have beneficial antimicrobial roles that can be exploited in controlling unwanted microbial growth.

  19. Squeezing Fact from Fiction about 100% Fruit Juice123

    Science.gov (United States)

    Clemens, Roger; Drewnowski, Adam; Ferruzzi, Mario G; Toner, Cheryl D; Welland, Diane

    2015-01-01

    Total fruit intake in the United States is ~1 cup equivalent per day, or one-half of the 2010 Dietary Guidelines for Americans recommendation for adults. Two-thirds of the fruit consumed is whole fruit and one-third is 100% juice. The nutritional value of whole fruit, with the exception of fiber and vitamin C, may be retained with appropriate juice production methods and storage conditions. One-hundred percent fruit juice consumption is associated with a number of health benefits, such as improved cardiovascular health and decreased obesity, although some of these and other potential benefits are controversial. Comprehensive analyses of the evidence by the Academy of Nutrition and Dietetics in 2014, the US Dietary Guidelines Advisory Committee in 2010, and the Australian Dietary Guidelines of 2013 concluded that 100% fruit juice is not related to adiposity in children when consumed in appropriate amounts for age and energy needs. However, some reports suggest the consumption of fruit juice contributes to unhealthful outcomes, particularly among children. A dietary modeling study on the best ways to meet the fruit intake shortfall showed that a combination of whole fruit and 100% juice improved dietary density of potassium and vitamin C without significantly increasing total calories. Notably, 100% juice intake was capped at amounts consistent with the 2001 American Pediatric Association guidance. The preponderance of evidence supports the position that 100% fruit juice delivers essential nutrients and phytonutrients, provides year-round access to a variety of fruits, and is a cost-effective way to help people meet fruit recommendations. PMID:25770266

  20. Squeezing fact from fiction about 100% fruit juice.

    Science.gov (United States)

    Clemens, Roger; Drewnowski, Adam; Ferruzzi, Mario G; Toner, Cheryl D; Welland, Diane

    2015-03-01

    Total fruit intake in the United States is ~1 cup equivalent per day, or one-half of the 2010 Dietary Guidelines for Americans recommendation for adults. Two-thirds of the fruit consumed is whole fruit and one-third is 100% juice. The nutritional value of whole fruit, with the exception of fiber and vitamin C, may be retained with appropriate juice production methods and storage conditions. One-hundred percent fruit juice consumption is associated with a number of health benefits, such as improved cardiovascular health and decreased obesity, although some of these and other potential benefits are controversial. Comprehensive analyses of the evidence by the Academy of Nutrition and Dietetics in 2014, the US Dietary Guidelines Advisory Committee in 2010, and the Australian Dietary Guidelines of 2013 concluded that 100% fruit juice is not related to adiposity in children when consumed in appropriate amounts for age and energy needs. However, some reports suggest the consumption of fruit juice contributes to unhealthful outcomes, particularly among children. A dietary modeling study on the best ways to meet the fruit intake shortfall showed that a combination of whole fruit and 100% juice improved dietary density of potassium and vitamin C without significantly increasing total calories. Notably, 100% juice intake was capped at amounts consistent with the 2001 American Pediatric Association guidance. The preponderance of evidence supports the position that 100% fruit juice delivers essential nutrients and phytonutrients, provides year-round access to a variety of fruits, and is a cost-effective way to help people meet fruit recommendations. © 2015 American Society for Nutrition.

  1. Bioefficacy of Morinda tinctoria and Pongamia glabra plant extracts against the malaria vector Anopheles stephensi (Diptera: Culicidae

    Directory of Open Access Journals (Sweden)

    D. Amerasan

    2015-04-01

    Full Text Available Mosquito-borne diseases have an economic impact, including loss in commercial and labour outputs, particularly in countries with tropical and subtropical climates; however, no part of the world is free from vector-borne diseases. The aim of the present study was to investigate the larvicidal, adulticidal and ovicidal activity of dried leaf chloroform, ethyl acetate, acetone, aqueous, and methanol extracts of Morinda tinctoria and Pongamia glabra against larvae of Anopheles stephensi (Diptera: Culicidae. Larvae were exposed to varying concentrations of plant extracts for 24 h. All extracts showed moderate larvicidal effects after 24 h of exposure; however, the highest larval mortality was found with the leaf methanol extracts of M. tinctoria and P. glabra against the larvae of A. stephensi lethal concentration (LC50=136.24 and 141.05 ppm; LC90=342.67 and 368.89 ppm, respectively. The results of the adulticidal activity assays of chloroform, ethyl acetate, acetone, aqueous, and methanol extracts of M. tinctoria and P. glabra showed significant mortality against larvae of A. stephensi. The methanol extract showed maximum activity compared with the other extracts. The greatest effect on mean percentage hatch in the ovicidal assays was observed 48 h post-treatment. Percent hatch was inversely proportional to the concentration of extract, and directly proportional to the number of eggs. A mortality of 100% was observed with 100-400 ppm methanol extracts and 200-400 ppm aqueous extracts of M. tinctoria, and 200-400 ppm aqueous and methanol extracts of P. glabra. This study provides the first report of the larvicidal, adulticidal and ovicidal activities of M. tinctoria and P. glabra plant extracts against the malaria vector, A. stephensi, representing an ideal eco-friendly approach for its control.

  2. Pseudomonas sp. xylanase for clarification of Mausambi and Orange fruit juice

    Science.gov (United States)

    Sharma, Pawan Kumar; Chand, Duni

    2012-07-01

    Xylanase can be usd for many Industrial applications and juice clarification is one of them. Pseudomonas sp. xylanase was used for fruit juice clarification in free State. Maximum amount of juice clarification was in case of Mausambi juice was observed at 40 C∞ and 52 hours, in case of free enzyme treated juice there is 46.9% increase in clarity and 1.7 fold increase in reducing sugars of the juice and enzyme dose was optimized as 8U with maximum flow rate of 6 ml/min at this dose. In case of orange juice in free enzyme treated juice maximum clarity was observed at 40 C∞ and 52 hours, juice was found to be 42.14 % clear with increase of 1.9 fold of reducing sugars, enzyme dose optimized was 8.06U with maximum flow rate of 0.86 ml/min.

  3. Quality Characteristics and Antioxidant Activity of Yogurt Supplemented with Aronia (Aronia melanocarpa) Juice

    Science.gov (United States)

    Nguyen, Linh; Hwang, Eun-Sun

    2016-01-01

    We investigated the quality characteristics and antioxidant activities of yogurt supplemented with 1%, 2%, and 3% aronia juice and fermented for 24 h at 37°C. The total acidity increased with increasing levels of aronia juice and incubation time. Lightness and yellowness of the yogurt decreased, but redness increased, with increasing aronia juice content and incubation time. The number of lactic acid bacteria (LAB) increased with increased incubation time, and yogurt containing 2% and 3% aronia juice showed higher LAB counts than 1% aroinia juice-supplemented yogurt. The total polyphenol and flavonoid contents increased proportionally with increasing levels of aronia juice. Antioxidant activity of aronia-containing yogurt was significantly higher than that of the control and increased proportionally with aronia juice concentration. Yogurt with 2% aronia juice had the best taste (P<0.05). Aronia juice may be a useful additive for improving the taste and antioxidant potential of yogurt. PMID:28078255

  4. Effect of probiotics on patulin removal from synbiotic apple juice.

    Science.gov (United States)

    Zoghi, Alaleh; Khosravi-Darani, Kianoush; Sohrabvandi, Sara; Attar, Hosein; Alavi, Sayed Abolhasan

    2017-06-01

    Studies have reported the occurrence of the mycotoxin patulin in apple products. The aim of this study was to produce synbiotic apple juice and investigate the detoxification of patulin by Lactobacillus acidophilus and Lactobacillus plantarum as probiotic strains. The impact of seven process variables on efficiency of toxin removal was investigated using Plackett-Burman design and presence of the surface-layer proteins as binding site of probiotics to patulin was confirmed during 6 weeks of cold storage. Results showed that the removal of patulin by probiotic bacteria from apple juice depends significantly (P juice. In the best conditions, 91.23% of initial patulin concentration was removed from juice during 6 weeks refrigerated storage. No significant difference was observed in organoleptic properties of the synbiotic apple juice and raw sample. In the best condition reported in this study, contaminated synbiotic apple juice by patulin will be safe for consumers after the first day of probiotic inoculation. © 2016 Society of Chemical Industry. © 2016 Society of Chemical Industry.

  5. Recovery of volatile fruit juice aroma compounds by membrane technology

    DEFF Research Database (Denmark)

    Bagger-Jørgensen, Rico; Meyer, Anne S.; Pinelo, Manuel

    2011-01-01

    The influence of temperature (10–45°C), feed flow rate (300–500L/h) and sweeping gas flow rate (1.2–2m3/h) on the recovery of berry fruit juice aroma compounds by sweeping gas membrane distillation (SGMD) was examined on an aroma model solution and on black currant juice in a lab scale membrane...... (Cpermeate/Cfeed) of the aroma compounds. At 45°C the most volatile and hydrophobic aroma compounds obtained the highest concentration factors: 12.1–9.3 (black currant juice) and 17.2–12.8 (model solution). With black currant juice a volume reduction of 13.7% (vol.%) at 45°C, 400L/h, resulted in an aroma...... the degradation of anthocyanins and polyphenolic compounds in the juice. Industrial relevanceHigh temperature evaporation is the most widely used industrial technique for aroma recovery and concentration of juices, but membrane distillation (MD) may provide for gentler aroma stripping and lower energy consumption...

  6. Moro orange juice prevents fatty liver in mice

    Institute of Scientific and Technical Information of China (English)

    Federico Salamone; Marco Giorgio; Fabio Galvano; Giovanni Li Volti; Lucilla Titta; Lidia Puzzo; Ignazio Barbagallo; Francesco La Delia; Shira Zelber-Sagi; Michele Malaguarnera; Pier Giuseppe Pelicci

    2012-01-01

    AIM:To establish if the juice of Moro,an anthocyaninrich orange,may improve liver damage in mice with diet-induced obesity.METHODS:Eight-week-old mice were fed a high-fat diet (HFD) and were administrated water or Moro juice for 12 wk.Liver morphology,gene expression of lipid transcription factors,and metabolic enzymes were assessed.RESULTS:Mice fed HFD displayed increased body weight,insulin resistance and dyslipidemia.Moro juice administration limited body weight gain,enhanced insulin sensitivity,and decreased serum triglycerides and total cholesterol.Mice fed HFD showed liver steatosis associated with ballooning.Dietary Moro juice markedly improved liver steatosis by inducing the expression of peroxisome proliferator-activated receptor-a and its target gene acylCoA-oxidase,a key enzyme of lipid oxidation.Consistently,Moro juice consumption suppressed the expression of liver X receptor-o and its target gene fatty acid synthase,and restored liver glycerol-3-phosphate acyltransferase 1 activity.CONCLUSION:Moro juice counteracts liver steatogenesis in mice with diet-induced obesity and thus may represent a promising dietary option for the prevention of fatty liver.

  7. Relationships between nutrients and sucrose concentrations in sugarcane juice and use of juice analysis for nutrient diagnosis in Japan

    Directory of Open Access Journals (Sweden)

    Kenta Watanabe

    2016-04-01

    Full Text Available Sugarcane is an important economic crop in southwest Japan, but its production is decreasing. To increase sugar production, both sugarcane yield and quality should be improved. Fertilizer management is one of the factors that influence sugarcane quality. We accordingly focused on nutrients present in sugarcane juice and attempted to identify the key factors affecting sugarcane quality. We collected sugarcane samples from 2013 to 2015 from all of the sugar mills in Japan and examined the relationships between juice nutrients and sucrose concentration. Juice analysis over 3 year showed that potassium (K+ and chloride (Cl− were the most abundant cation and anion in the juice and that both negatively correlated with the sucrose concentration. K+ and Cl− concentrations significantly varied depending on production areas and those with higher K+ and Cl− concentrations had a low sucrose concentration. This finding suggests that sugarcane in those areas may have been supplied with these two ions in excess. Electrical conductivity (EC in the juice always positively correlated with K+ and Cl− concentrations. EC may thus be a reliable indicator of K+ and Cl− concentrations and could be used for nutrient diagnosis because of its ease of measurement. For improving sugarcane quality, we recommend that potassium chloride, which supplies both K+ and Cl− and is a commonly used potassium fertilizer for sugarcane production in Japan, should be used in lower quantities in a year following one in which the EC of sugarcane juice at harvest is found to be high.

  8. The Effects of Glucose Therapy Agents-Apple Juice, Orange Juice, and Cola-on Enteral Tube Flow and Patency.

    Science.gov (United States)

    Steinberg, Daphna J; Montreuil, Jasmine; Santoro, Andrea L; Zettas, Antonia; Lowe, Julia

    2016-06-01

    To develop evidence-based hypoglycemia treatment protocols in patients receiving total enteral nutrition, this study determined the effect on enteral tube flow of glucose therapy agents: apple juice, orange juice, and cola, and it also examined the effects of tube type and feed type with these glucose therapy agents. For this study, 12 gastrostomy tubes (6 polyethylene and 6 silicone) were set at 50 mL/h. Each feeding set was filled with Isosource HN with fibre or Novasource Renal. Each tube was irrigated with 1 glucose therapy agent, providing approximately 20 g of carbohydrate every 4 h. Flow-rate measurements were collected at 2 h intervals. The results showed that the glucose therapy agent choice affected flow rates: apple juice and cola had higher average flow rates than orange juice (P = 0.01). A significant difference was found between tube type and enteral formula: polyethylene tubes had higher average flow rates than silicone tubes (P rates than Novasource Renal (P = 0.01). We concluded that apple juice and cola have less tube clogging potential than orange juice, and thus may be considered as primary treatment options for hypoglycemia in enterally fed patients. Polyethylene tubes and Isosource HN with fibre were less likely to clog than silicone tubes and Novasource Renal.

  9. Microfiltration of red berry juice with thread filters: Effects of temperature, flow and filter pore size

    DEFF Research Database (Denmark)

    Bagger-Jørgensen, Rico; Casani, Sandra Dobon; Meyer, Anne Boye Strunge

    2002-01-01

    ) on the transmembrane pressure, juice turbidity, protein, sugar, and total phenols levels was evaluated in a lab scale microfiltration unit employing statistically designed factorial experiments. Thread microfiltration reduced significantly the turbidity of both juices. For blackcurrant juice, in all experiments......, the turbidity was immediately reduced to the level required for finished juice without compromising either the protein, the sugar or the phenols content. High flow rates increased the turbidity in blackcurrant juice, but did not affect cherry juice quality. Filtomat(R) thread microfiltration therefore appears...

  10. Glutathione suppresses the enzymatic and non-enzymatic browning in grape juice.

    Science.gov (United States)

    Wu, Shengjun

    2014-10-01

    Browning tends to occur in grape juice during processing and storage and decreases the commercial value of it. Thus, browning inhibition is an important objective for manufacturers. This study aims to investigate the efficacy of glutathione as a browning inhibitor for use on grape juice. Grape juice browning treated with glutathione was monitored during processing and accelerated browning. 0.04% of glutathione inhibited 99.4% of the polyphenoloxidase activity in the grape juice. Consequently, during processing at room temperature and accelerated browning at 80 °C, the browning in the grape juice treated with glutathione was significantly lower than that in the control (pbrowning inhibitor used in grape juice.

  11. Chemical and antioxidant properties of snake tomato (Trichosanthes cucumerina) juice and Pineapple (Ananas comosus) juice blends and their changes during storage.

    Science.gov (United States)

    Bamidele, Oluwaseun P; Fasogbon, Mofoluwaso B

    2017-04-01

    Juice blends made from the mixture of snake tomato (Trichosanthes cucumerina) and Pineapple (Ananas comosus) fruits were analyzed for pH, antioxidant properties, total titratable acidity, vitamin C, lycopene and total phenolic contents after different blend ratios were made. The addition of snake tomato juice increased the vitamin C, total carotene, lycopene and antioxidant properties of the juice blends. The radical scavenging properties of juice blends containing a higher ratio of snake tomato were higher and samples stored at room temperature (29°C) showed an increase in antioxidant properties compared to samples stored at 4°C. In conclusion, snake tomato juice up to 50% may be added to Pineapple juice to make a healthy juice blend. Copyright © 2016 Elsevier Ltd. All rights reserved.

  12. Patulin surveillance in apple cider and juice marketed in Michigan.

    Science.gov (United States)

    Harris, Kerri L; Bobe, Gerd; Bourquin, Leslie D

    2009-06-01

    Patulin is the most common mycotoxin found in apples and apple juices. The objective of this study was to determine the concentrations of patulin in (i) apple cider produced and marketed by Michigan apple cider mills during the fall seasons of 2002 to 2003 and 2003 to 2004 and (ii) apple juice and cider, including shelf-stable products, marketed in retail grocery stores in Michigan throughout 2005 and 2006. End product samples (n=493) obtained from 104 Michigan apple cider mills were analyzed for patulin concentration by using solid-phase extraction followed by high-performance liquid chromatography. Patulin was detected (> or =4 microg/liter) in 18.7% of all cider mill samples, with 11 samples (2.2%) having patulin concentrations of > or =50 microg/liter. A greater percentage of cider samples obtained from mills using thermal pasteurization contained detectable patulin (28.4%) than did those from mills using UV light radiation (13.5%) or no pathogen reduction treatment (17.0%). Among retail grocery store samples (n=159), 23% of apple juice and cider samples contained detectable patulin, with 18 samples (11.3%) having patulin concentrations of > or =50 microg/liter. The U.S. Food and Drug Administration (FDA) action level for patulin is 50 microg/kg. Some apple juice samples obtained from retail grocery stores had exceptionally high patulin concentrations, ranging up to 2700 microg/liter. Collectively, these results indicate that most apple cider and juice test samples from Michigan were below the FDA action level for patulin but that certain apple cider and juice processors have inadequate controls over patulin concentrations in final products. The industry, overall, should focus on improved quality of fruit used in juice production and improve culling procedures to reduce patulin concentrations.

  13. Juice-associated outbreaks of human illness in the United States, 1995 through 2005.

    Science.gov (United States)

    Vojdani, Jazmin D; Beuchat, Larry R; Tauxe, Robert V

    2008-02-01

    Outbreaks of illness associated with consumption of fruit juice have been a growing public health problem since the early 1990s. In response to epidemiologic investigations of outbreaks in which juice was implicated, the U.S. Food and Drug Administration implemented process control measures to regulate the production of fruit juice. The final juice regulation, which became effective in 2002, 2003, and 2004, depending on the size of the business, requires that juice operations comply with a hazard analysis critical control point (HACCP) plan. The Centers for Disease Control and Prevention (CDC) receives reports of food-associated outbreaks of illness. We reviewed fruit juice-associated outbreaks of illness reported to the CDC's Foodborne Outbreak Reporting System. From 1995 through 2005, 21 juice-associated outbreaks were reported to CDC; 10 implicated apple juice or cider, 8 were linked to orange juice, and 3 involved other types of fruit juice. These outbreaks caused 1,366 illnesses, with a median of 21 cases per outbreak (range, 2 to 398 cases). Among the 13 outbreaks of known etiology, 5 were caused by Salmonella, 5 by Escherichia coli O157:H7, 2 by Cryptosporidium, and one by Shiga toxin-producing E. coli O111 and Cryptosporidium. Fewer juice-associated outbreaks have been reported since the juice HACCP regulation was implemented. Some juice operations that are exempt from processing requirements or do not comply with the regulation continue to be implicated in outbreaks of illness.

  14. Flash Profile for rapid descriptive analysis in sensory characterization of passion fruit juice

    Directory of Open Access Journals (Sweden)

    Flávia Daiana Montanuci

    2015-07-01

    Full Text Available The Flash Profile is a descriptive analysis method derived from Free-Choice Profile, in which each taster chooses and uses his/her own words to evaluate the product while comparing several attributes. Four passion fruit juices were analyzed, two juices were produced with concentrated juice, one with pulp and one with reconstituted juice; all juices had different levels of sugar, some had gum and dyes. This study aimed to evaluate the physicochemical properties (color, titratable acidity and solid content as well as sensory analysis like Flash profile and affective test. In physicochemical characterization and in Flash Profile, the juice A (pulp had higher solid content and consistence, the juice B (concentrated juice was the least acidic and presented the lowest value of soluble solids and presented strong aroma and flavor of passion-fruit, the juice C (reconstituted juice was pale yellow and showed artificial flavor and the juice D (concentrated juice was the most acidic, consistent with the natural flavor. In the acceptance test, all the juices scored 5-6, indicating that panelists tasters neither liked nor disliked. Flash Profile proved to be an easy and rapid technique showing a good correlation between panelists and the attributes and confirmed the results of physicochemical characterization.

  15. Identification of specific microorganisms in fresh squeezed street vended fruit juices

    Directory of Open Access Journals (Sweden)

    K Sahithi Reddy

    2016-01-01

    Full Text Available Introduction: In developing country like India, street foods such as salads and fresh cut fruits are widely consumed. Among all street foods, fruit juices are common beverages, consumed more because of higher consumer preference both in terms of taste and health. Moreover, there is a dearth of Indian studies on contamination of street vended fruit juices. Aim: To determine the pH and specific microorganisms in freshly squeezed street vended fruit juices. Materials and Methods: Four fruit juices i.e., Grapes, Sweet Lime, Pineapple and Sapota were chosen for the study. Juices were collected in summer season in months between April and June 2013. Ten samples of 50 ml each fruit juice was collected in sterile bottles from various street vendors of Dilshuknagar area of Hyderabad city. Transportation of samples to Food Toxicology laboratory, National Institute of Nutrition was done in the ice box and processing was done within 2–4 h. Results: All juices showed bacterial contamination except one sample of grape juice. Pineapple juice samples showed the high bacterial contamination with all samples positive for fecal coliforms and Shigella spp. (100%. Salmonella spp. was detected only in one sample of Sapota juice (10%. Significant difference among fruit juices for prevalence of microorganisms was seen only for Escherichia coli (P = 0.03 with least count in Grape juice (20%. Conclusion: Freshly squeezed street vended fruit juices were contaminated with pathogenic bacteria, which significantly attributed to public health problem.

  16. Removal of phenolic compounds in pomegranate juices using ultrafiltration and laccase-ultrafiltration combinations.

    Science.gov (United States)

    Alper, Neslihan; Acar, Jale

    2004-06-01

    Phenolic compounds of fruit juices are responsible for haze and sediment formation as well as for color, bitterness and astringency. The influence of ultrafiltration (UF) and laccase-UF combination was investigated on phenolic contents of pomegranate juices and on filtration output. Laccase-treated and then ultrafiltered pomegranate juices have shown a rapid increase in their color, when compared to only ultrafiltered (control) samples. Kinetic parameters of laccase were also determined. During the oxidation period, the changes occurring in pomegranate juices were estimated from phenolic contents, color and anthocyanin measurements. Results have shown that laccase oxidation produced a significant decrease in phenolic content of pomegranate juices while juice color the increased. However, in recent literatures, the possibility to remove polyphenols in apple juices was reported. We decided in this study that laccase treatment can not be applied due to the loss of natural red color and unwanted dark brownish color formation in pomegranate juice.

  17. Antioxidant effects of Citrus aurantifolia (Christm) juice and peel extract on LDL oxidation.

    Science.gov (United States)

    Boshtam, Maryam; Moshtaghian, Jamal; Naderi, Gholamali; Asgary, Seddigheh; Nayeri, Hashem

    2011-07-01

    We studied the antioxidant effects of fresh juice and peel extract of Citrus aurantifolia (Christm). Low density lipoprotein (LDL) was separated from one hypercholesterolemic human serum by modified Bronzert and Brewer procedure. Oxidation of LDL was measured at 234 nm against 0, 5, 10, 20, 25, 30 and 40 μl of fresh lime juice and 0, 5, 10, 15 and 20 μl of peel polyphenolic extract solution in DMSO. 5 μl of lime juice didn't change LDL oxidation. 10 μl of juice inhibited LDL oxidation, and with increasing the juice concentration, LDL was oxidized faster. The higher concentrations of peel extract prevented LDL oxidation better than the lower ones. Both juice and peel demonstrated antioxidant properties, but the excessive consumption of lime juice seems not to be beneficial. Regarding the intensity and type of flavonoids, lime juice and peel may show different effects.

  18. Quality Characteristics and Antioxidant Activity of Yogurt Supplemented with Aronia (Aronia melanocarpa) Juice.

    Science.gov (United States)

    Nguyen, Linh; Hwang, Eun-Sun

    2016-12-01

    We investigated the quality characteristics and antioxidant activities of yogurt supplemented with 1%, 2%, and 3% aronia juice and fermented for 24 h at 37°C. The total acidity increased with increasing levels of aronia juice and incubation time. Lightness and yellowness of the yogurt decreased, but redness increased, with increasing aronia juice content and incubation time. The number of lactic acid bacteria (LAB) increased with increased incubation time, and yogurt containing 2% and 3% aronia juice showed higher LAB counts than 1% aroinia juice-supplemented yogurt. The total polyphenol and flavonoid contents increased proportionally with increasing levels of aronia juice. Antioxidant activity of aronia-containing yogurt was significantly higher than that of the control and increased proportionally with aronia juice concentration. Yogurt with 2% aronia juice had the best taste (Pjuice may be a useful additive for improving the taste and antioxidant potential of yogurt.

  19. [RP-HPLC fingerprint evaluating different ginger juice as processing material].

    Science.gov (United States)

    Zhang, Li; Wang, Zhi-Min; Wang, Wei-Hao; Gao, Hui-Min

    2008-05-01

    To establish a method for comparing the differences between fresh and dried ginger juice. The RP-HPLC fingerprint method was performed on an Alltech C18 column (4.6 mm x 250 mm, 5 microm) with mobile phase in gradient elution composed of A-acetonitrie and B-water at a flow rate: 0.8 mL x min(-1). The detecting wavelength was 280 nm, and the column temperature 25 degrees C. There was no significant difference among the same breed ginger juice of different batches. But there was significant difference between crushed ginger juice and the boiled juice. Trytophan, 6-gingerol were common constituents of the three kinds of ginger juice, the fresh ginger and the dry ginger. Besides, 6-shogaol emerged in the boiled juice. The RP-HPLC fingerprints spectrum can be used to distinguish different ginger juices. And the crushed juice of fresh ginger have the same chemical consititents with the fresh ginger.

  20. New vegetable and fruit-vegetable juices treated by high pressure

    Science.gov (United States)

    Gabrovská, Dana; Ouhrabková, Jarmila; Rysová, Jana; Laknerová, Ivana; Fiedlerová, Vlasta; Holasová, Marie; Winterová, Renata; Průchová, Jiřina; Strohalm, Jan; Houška, Milan; Landfeld, Aleš; Erban, Vladimír; Eichlerová, Eva; Němečková, Irena; Kejmarová, Marie; Bočková, Pavlína

    2012-03-01

    The aim of this work was to find sensory suitable combinations of not commonly used vegetables, that is, cabbage, celeriac and parsnip, into mixed fruit-vegetable juices, two-species vegetable juices and vegetable juices with whey. These juices might have the potential to offer consumers new, interesting, tasty and nutritional products. Another interesting variation could be preparation of vegetable juices in combination with sweet whey. Nutritional and sensory evaluations were carried out using juices prepared in the laboratory. The total phenolic content, in addition to ascorbic acid and antioxidant activity, was determined. The developed juices with high nutritional value should increase very low fruit and vegetable consumption in the Czech population. The prepared juices were high pressure pasteurized (410 MPa). This technique retains the desired levels of important nutritional substances, while being destructive to live microbial cell structure. The germination of spores is suppressed by low pH value.