Using virtual reality technology for the planning of decommissioning of Fugen NPS

International Nuclear Information System (INIS)

Iguchi, Yukihiro; Sundling, Carl-Victor

1999-01-01

Fugen is a 165 MWe, heavy water moderated, light-water cooled, pressure-tube type reactor, which started the commercial operation in 1979. The operation of the plant will end by March 2003 and the tasks of decommissioning will start under the policy of the Japanese government. The basic requirement shows the Fugen Decommissioning Project (FDP) should be transparent with sharing international and domestic experience and knowledge, rational using existing technologies, economical with detailed waste evaluation, environmentally friendly by resource recycling and beneficial for local community. Based on the requirements, we have started the planning of decommissioning and found that the collection of sufficient data concerning the plant such as radioactivity inventory and weight of the material is important and optimization of the plan is necessary for the cost reduction. On the other hand, based on the experience of the decommissioning of Japan Power Demonstration Reactor (JPDR), Japan Atomic Energy Research Institute (JAERI) developed the Code System for Management of Reactor Decommissioning (COSMARD), which evaluates project management data such as costs, manpower needs, radiation exposure to workers and waste mass based on the plant data and experienced data concerning the decommissioning in the past. COSMARD is used commonly in Japan and contributes to the optimization of the decommissioning planning including the commercial reactors. The progress of the Virtual Reality (VR) technology shows new possibility of the planning of decommissioning. For example, the simulation of the dismantling process with special devices in the high radiation area is possible with the VR technique. The estimation of radiation exposure of workers may be another target for the VR system with human behavior (author) (ml)

Document collection of the 28th technical special committee on Fugen decommissioning

International Nuclear Information System (INIS)

Kutsuna, Hideki; Iwai, Hiroki; Mizui, Hiroyuki; Kadowaki, Haruhiko; Nakamura, Yasuyuki

2014-02-01

Fugen Decommissioning Engineering Center, in planning and carrying out our decommissioning technical development, has been establishing “Technical special committee on Fugen decommissioning” which consists of the members well-informed, aiming to make good use of Fugen as a place for technological development which is opened inside and outside the country, as the central point in the energy research and development base making project of Fukui prefecture, and to utilize the outcome in our decommissioning to the technical development effectively. This report compiles presentation materials “The Current Situation of Fugen Decommissioning”, “The Current Status of the Cutting Test toward the Practical Use of Laser Cutting Technology and the Future Plan”, “Study on Radioactive Substance Osmosis for Basis Concrete of Equipment”, “Verification Tests of the Room-Temperature Vacuum Drying and the Evaluation Method of Residual Amount of Heavy Water in the Tritium Removal” and “Report on the FUGEN Efforts of R and D for Decommissioning of the Fukushima Daiichi NPS - Applicability Test of Thermal and Mechanical Cutting Technology for the Dismantlement of the Internal Core of Fukushima Daiichi NPS -”, presented in the 28th Technical special committee on Fugen decommissioning which was held on September 24, 2013. (author)

Lessons-learned from ongoing decommissioning project of Fugen NPS

International Nuclear Information System (INIS)

Tezuka, M.; Koda, Y.; Iguchi, Y.; Kato, Y.; Yanagihara, S.

2017-01-01

Advanced Thermal Reactor (ATR) Fugen is a 557 MWt, 165 MWe, heavy water moderated, light-water cooled, pressure-tube type reactor. In 2003, Fugen was shut down after ca 25 years operation, and started decommissioning activity from 2008. In the initial period of decommissioning, we have been dismantling from turbine systems because of their little contamination. In general, most difficult process of dismantling of nuclear power plant is the dismantlement of the reactor core because the radiation rate of the reactor core is very high, e.g., it is over 200 Sv/hr in the Fugen's case. Our plan of dismantlement of the core is from about 2022. The core area has some features that the structure is narrow and complicated by tube-cluster structure that contains 224 fuel channels with both the pressure and the calandria tubes coaxially in each channel. The radiation shielding area is laminated structure composed of up to 150 mm thickness of carbon steel. And the structure of the reactor, which is made of various materials such as stainless steel, carbon steel, zirconium alloy and aluminum. In particular, the core area is planning to be dismantled under water by remote controlled machines in order to shield the radiation around the core and prevent airborne dust generated by the cutting considering the usage of Zr alloy which is likely to be oxidized. In consideration of above, the cutting methods were selected for dismantling the reactor core in order to shorten the dismantling term and reduce the secondary waste. The candidate cutting method options were decreased based on the results of the researches on achievement of the cutting methods domestically and internationally. Finally, the laser cutting method was selected for dismantling the core area and shielding area, and diamond wire saw was also selected for dismantling the shielding area applicable to concrete with metal liner, based on the results of some cutting tests. The laser cutting method has many advantages, e

Safety analysis for 'Fugen'

International Nuclear Information System (INIS)

1997-10-01

The improvement of safety in nuclear power stations is an important proposition. Therefore also as to the safety evaluation, it is important to comprehensively and systematically execute it by referring to the operational experience and the new knowledge which is important for the safety throughout the period of use as well as before the construction and the start of operation of nuclear power stations. In this report, the results when the safety analysis for ''Fugen'' was carried out by referring to the newest technical knowledge are described. As the result, it was able to be confirmed that the safety of ''Fugen'' has been secured by the inherent safety and the facilities which were designed for securing the safety. The basic way of thinking on the safety analysis including the guidelines to be conformed to is mentioned. As to the abnormal transient change in operation and accidents, their definition, the events to be evaluated and the standards for judgement are reported. The matters which were taken in consideration at the time of the analysis are shown. The computation programs used for the analysis were REACT, HEATUP, LAYMON, FATRAC, SENHOR, LOTRAC, FLOOD and CONPOL. The analyses of the abnormal transient change in operation and accidents are reported on the causes, countermeasures, protective functions and results. (K.I.)

Improvement of INVS Measurement Uncertainty for Pu and U-Pu Nitrate Solution

Energy Technology Data Exchange (ETDEWEB)

Swinhoe, Martyn Thomas [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Menlove, Howard Olsen [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Marlow, Johnna Boulds [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Makino, Risa [Japan Atomic Energy Agency (JAEA), Tokai (Japan); Nakamura, Hironbu [Japan Atomic Energy Agency (JAEA), Tokai (Japan)

2017-04-27

In the Tokai Reprocessing Plant (TRP) and the Plutonium Conversion Development Facility (PCDF), a large amount of plutonium nitrate solution which is recovered from light water reactor (LWR) and advanced thermal reactor (ATR), FUGEN are being stored. Since the solution is designated as a direct use material, the periodical inventory verification and flow verification are being conducted by Japan Safeguard Government Office (JSGO) and International Atomic Agency (IAEA).

Semi-empirical evaluation studies on PCMI for the Fugen fuel rod

International Nuclear Information System (INIS)

Domoto, Kazushige; Kaneko, Mitsunobu; Takeuchi, Kiyoshi.

1980-03-01

Fugen, 165 MWe prototype of a heavy water moderated boiling water cooled reactor, has been well operated since March 1979. In order to establish PCIOMR for Fugen fuels semi-empirical evaluation code to analyze PCMI during power transient of the fuel rod has been developed. In this paper, followings are described 1) general scope of the development work 2) description of the modelling 3) some results of analysis on out pile and in pile tests. (author)

The plutonium utilization in thermal and fast reactor in Japan

International Nuclear Information System (INIS)

Amanuma, T.; Uematsu, K.

1977-01-01

The nuclear power development in Japan is rather extensive one, and the installed nuclear power capacity is expected to reach 49,000 MWe by 1985 and possibly to reach 170,000 MWe by 2000 according to a prediction. Currently istalled nuclear power is mainly based on Light Water type Reactor, and this trend is expected to persist for the time-being. The plutonium produced by LWR will be accumulated to 20 tons by 1985 and to more than 200 tons by 2000. If the produced plutonium will simply be stored, it will raise the economic pressure to utilities and the management and physical protection problems associated with plutonium storing. Therefore, it is not too wise simply to store plutonium in a locked vault. In Japan, there are three ways of solving these problems which are currently worked out. There is no doubt that the best solution is to use plutonium in fast reactors. To reach this goal, an Experimental Fast Reactor ''JOYO''has been constructed and it is waiting for criticality in very near future. A prototype fast breeder reactor ''MONJU'', which is designed for about 300 MWe, is nearing to the last stage of the design work. The start of its construction will take place in a few yesars. The domonstration fast breeder reactor will come next to ''MONJU'' and the large scale commercial use of fast breeder reactor is expected to start around 1995. To anwer the near-term need for plutonium utilization, two technologies, which are equally important to Japan, are currently developed. One is the recycle use of plutonium into LWR. This technology has long been jointly developed by research organizations and utilities. Some of fuel irradiation data are already obtained and the physics study has also been extensive. The application of this technology is expected to start about 1987. The other is to burn plutonium in an Advanced Thermal Reactor (D 2 O moderated, Boiling Water Cooled) which shows better characteristics of using plutonium. The 160 MWe ''Fugen'' is a prototype

Start-up test of the prototype heavy water reactor 'FUGEN', (1)

International Nuclear Information System (INIS)

Ando, Hideki; Kawahara, Toshio

1982-01-01

The advanced thermal prototype reactor ''Fugen'' is a heavy water-moderated, boiling light water-cooled power reactor with electric output of 165 MW, which has been developed since 1966 as a national project. The start-up test was begun in March, 1978, being scheduled for about one year, and in March, 1979, it passed the final pre-use inspection and began the full scale operation. In this paper, the result of the start-up test of Fugen is reported. From the experience of the start-up test of Fugen, the following matters are important for the execution of start-up test. 1) Exact testing plan and work schedule, 2) the organization to perform the test, 3) the rapid evaluation of test results and the reflection to next testing plan, and 4) the reflection of test results to rated operation, regular inspection and so on. In the testing plan, the core characteristics peculiar to Fugen, and the features of heavy water-helium system, control system and other equipment were added to the contents of the start-up test of BWRs. The items of the start-up test were reactor physics test, plant equipment performance test, plant dynamic characteristic test, chemical and radiation measurement, and combined test. The organization to perform the start-up test, and the progress and the results of the test are reported. (Kako, I.)

Drying of heavy water system and works of charging heavy water in Fugen

International Nuclear Information System (INIS)

Matsushita, Tadashi; Iijima, Setsuo

1980-01-01

The advanced thermal reactor ''Fugen'' is the first heavy water-moderated, boiling light water-cooled nuclear reactor for power generation in Japan. It is a large heavy water reactor having about 130 m 3 of heavy water inventory and about 300 m 3 of helium space as the cover gas of the heavy water system. The heavy water required was purchased from FRG, which had been used for the power output test in the KKN, and the quality was 99.82 mol % mean heavy water concentration. The concentration of heavy water for Fugen used for the nuclear design is 99.70 mol%, and it was investigated how heavy water can be charged without lowering the concentration. The matters of investigation include the method of bringing the heavy water and helium system to perfect dryness after washing and light water test, the method of confirming the sufficient dryness to prevent the deterioration, and the method of charging heavy water safely from its containers. On the basis of the results of investigation, the actual works were started. The works of drying the heavy water and helium system by vacuum drying, the works of sampling heavy water and the result of the degree of deterioration, and the works of charging heavy water and the measures to the heavy water remaing in the containers are described. All the works were completed safely and smoothly. (J.P.N.)

CAI and training system for the emergency operation procedure in the advanced thermal reactor, FUGEN

International Nuclear Information System (INIS)

Kozaki, T.; Imanaga, K.; Nakamura, S.; Maeda, K.; Sakurai, N.; Miyamoto, M.

2003-01-01

In the Advanced Thermal Reactor (ATR ) of the JNC, 'FUGEN', a symptom based Emergency Operating Procedure (EOF) was introduced in order to operate Fugen more safely and it became necessary for the plant operators to master the EOF. However it took a lot of time for the instructor to teach the EOP to operators and to train them. Thus, we have developed a Computer Aided Instruction (CAI) and Training System for the EOP, by which the operators can learn the EOP and can be trained. This system has two major functions, i.e., CAI and training. In the CAI function, there are three learning courses, namely, the EOP procedure, the simulation with guidance and Q and A, and the free simulation. In the training function, all of necessary control instruments (indicators, switches, annunciators and so forth) and physics models for the EOP training are simulated so that the trainees can be trained for all of the EOPs. In addition, 50 kinds of malfunction models are installed in order to perform appropriate accident scenarios for the EOP. The training of the EOP covers the range from AOO (Anticipated Operational Occurrence) to Over-DBAs (Design Based Accidents). This system is built in three personal computers that are connected by the computer network. One of the computers is expected to be used for the instructor and the other two are for the trainees. The EOP is composed of eight guidelines, such as 'Reactor Control' and 'Depression and Cooling', and the operation screens which are corresponded to the guidelines are respectively provided. According to the trial, we have estimated that the efficiency of the learning and the training would be improved about 30% for the trainee and about 75% for the instructor in the actual learning and training. (author)

Mox fuel utilization in ATR

OpenAIRE

下村 和生; 川太 徳夫

1987-01-01

ATR, a heavy-water moderated boiling-light-water cooled reactor developed in Japan, is a unique reactor with out-standing flexibility regarding nuclear fuel utilization, because it has superior properties concerning the utilization of plutonium, recovered uranium and depleted uranium. The development of this type of reactor is expected to contribute both to the stable supply of energy and to the establishment of plutonium utilization in Japan. Much effort has been and will be made on the deve...

A feasibility study on worksite visualization system using augmented reality for Fugen NPP

International Nuclear Information System (INIS)

Izumi, Masanori; Shimoda, Hiroshi; Ishii, Hirotake

2010-01-01

Fugen Nuclear Power Plant, Advanced Thermal Reactor, was permanently shut down in March 2003 and it is at the decommissioning stage. Decommissioning Engineering Support System, DEXUS, has been developed to help planning of the optimal dismantling process and for carrying out the dismantling work safely and efficiently. Worksite Visualization System (WVS), as part of Dismantling Work Support System of DEXUS, has been developed to support the field workers to deal with the information on the dismantling facilities comprehensibly and intuitively. In this article, outline of the dismantling process of Fugen is first introduced, then a feasibility study on WVS is described. (author)

Development of decommissioning engineering support system (DEXUS) of the Fugen Nuclear Power Station

International Nuclear Information System (INIS)

Iguchi, Yukihiro; Kanehira, Yoshiki; Tochibana, Mitsuo

2004-01-01

The Fugen Nuclear Power Station (NPS) was shut down permanently in March 2003, and preparatory activities are underway to decommission the Fugen NPS. An engineering system to support the decommissioning is being developed to create a dismantling plan using state-of-art software such as 3-dimensional computer aided design (3D-CAD) and virtual reality (VR). In particular, an exposure dose evaluation system using VR has been developed and tested. The total system can be used to quantify radioactive waste, to visualize radioactive inventory, to simulate the dismantling plan, to evaluate workload in radiation environments and to optimize the decommissioning plan. The system will also be useful for educating and training workers and for gaining public acceptance. (author)

Operation management of the prototype heavy water reactor 'Fugen'

International Nuclear Information System (INIS)

Muramatsu, Akira; Takei, Hiroaki; Iwanaga, Shigeru; Noda, Masao; Hara, Hidemi

1983-01-01

The advanced thermal reactor Fugen power station has continued almost smooth operation since it began the full scale operation as the first homemade power reactor in Japan in March, 1979. In the initial period of operation, some troubles were experienced, but now, it can be said that the operational techniques of heavy water-moderated, boiling light water-cooled, pressure tube type reactors have been established, through the improvement of the operational method and equipment, and the operational experience. Also, the verification of the operational ability, maintainability, reliability and safety of this new type reactor, that is the mission of the prototype reactor, achieved steadily the good results. Hereafter, the verification of operational performance is the main objective because it is required for the design, construction and operation of the demonstration reactor. The organization for the operation management and operation, the communication at the time of the abnormality, the operation of the plant, that is, start up, stop and the operation at the rated output, the works during plant stoppage, the operation at the time of the plant abnormality, the operation of waste treatment facility and others, the improvement of the operational method, and the education and training of operators are reported. (Kako, I.)

Heavy water radiolysis and chemistry control of the Fugen Nuclear Power Station

International Nuclear Information System (INIS)

Ibuki, Y.; Kitabata, T.; Kato, T.

1989-01-01

A computer analysis for heavy water radiolysis clarified the mechanism of the heavy water radiolysis rate change with impurities in the heavy water and cover gas, helium. The mechanism is supported by over ten years' operational data of the heavy water radiolysis in the Fugen nuclear power station. (author)

Operation management of the prototype heavy water reactor 'Fugen'

Energy Technology Data Exchange (ETDEWEB)

Muramatsu, Akira; Takei, Hiroaki; Iwanaga, Shigeru; Noda, Masao; Hara, Hidemi (Power Reactor and Nuclear Fuel Development Corp., Tokyo (Japan))

1983-09-01

The advanced thermal reactor Fugen power station has continued almost smooth operation since it began the full scale operation as the first homemade power reactor in Japan in March, 1979. In the initial period of operation, some troubles were experienced, but now, it can be said that the operational techniques of heavy water-moderated, boiling light water-cooled, pressure tube type reactors have been established, through the improvement of the operational method and equipment, and the operational experience. Also, the verification of the operational ability, maintainability, reliability and safety of this new type reactor, that is the mission of the prototype reactor, achieved steadily the good results. Hereafter, the verification of operational performance is the main objective because it is required for the design, construction and operation of the demonstration reactor. The organization for the operation management and operation, the communication at the time of the abnormality, the operation of the plant, that is, start up, stop and the operation at the rated output, the works during plant stoppage, the operation at the time of the plant abnormality, the operation of waste treatment facility and others, the improvement of the operational method, and the education and training of operators are reported.

Study on applicability of evaluation model of manpower needs for dismantling of equipments in FUGEN-2. Preparation and clean-up process in 3rd/4th feedwater heater room

International Nuclear Information System (INIS)

Shibahara, Yuji; Izumi, Masanori; Nanko, Takashi; Tachibana, Mitsuo

2011-06-01

Manpower needs for the preparation and clean-up process on the dismantling of equipments in FUGEN 3rd/4th feedwater heater room conducted in 2008 were calculated with the management data evaluation system: PRODIA Code, and it was inspected whether a conventional evaluation model had applicability for large nuclear facilities such as FUGEN or not. It was confirmed that the conventional evaluation model had no applicability for FUGEN causing by the difference in the plant scale between JPDR and FUGEN bringing expansion of working area. The difference between the actual data and the calculated value was improved by reviewing of the evaluation model, and this reviewing process also brought a new evaluation model. (author)

Non-viral genetic transfection of rat Schwann cells with FuGENE HD© lipofection and AMAXA© nucleofection is feasible but impairs cell viability.

Science.gov (United States)

Kraus, Armin; Täger, Joachim; Kohler, Konrad; Haerle, Max; Werdin, Frank; Schaller, Hans-Eberhard; Sinis, Nektarios

2010-11-01

To determine transfection efficiency of FuGENE HD© lipofection and AMAXA© nucleofection on rat Schwann cells (SC). The ischiadic and median nerves of 6-8 week old Lewis rats were cultured in modified melanocyte-growth medium. SCs were genetically transfected with green fluorescent protein (GFP) as reporter gene using FuGENE HD© lipofection and AMAXA© nucleofection. Transfection rates were determined by visualization of GFP fluorescence under fluorescence microscopy and cell counting. Transfected cell to non-transfected cell relation was determined. Purity of Schwann cell culture was 88% as determined by immunohistologic staining. Transfection rate of FuGENE HD© lipofection was 2%, transfection rate of AMAXA© nucleofection was 10%. With both methods, Schwann cells showed pronounced aggregation behavior which made them unfeasible for further cultivation. Settling of Schwann cells on laminin and poly-L-ornithine coated plates was compromised by either method. Non-viral transfection of rat SC with FuGENE HD© lipofection and AMAXA© nucleofection is basically possible with a higher transfection rate for nucleofection than for lipofection. As cell viability is compromised by either method however, viral transfection is to be considered if higher efficiency is required.

Power-up of Fugen reactor and development of demonstration plant

International Nuclear Information System (INIS)

Sawai, Sadamu; Akebi, Michio; Yazaki, Akira.

1979-06-01

The Fugen Nuclear Power Station is the 165 MWe prototype plant characterized by heavy water-moderated, boiling light water-cooled, pressure tube type, and was developed by the Power Reactor and Nuclear Fuel Development Corporation, Japan. The plant went into commercial operation on March 20, 1979, in Tsuruga, Fukui Prefecture. Some delay in the overall schedule occurred due to the shortage of cement caused by the oil crisis, more stringent regulations as the result of stress corrosion cracking experienced in BWRs, and design modifications. All functional tests, the final check-up of the whole plant, and remaining modifying works had been completed by March 10, 1978. The minimum criticality was achieved with 22 mixed oxide fuel assemblies on March 20, 1978. Thereafter, the tests on reactor physics, plant dynamics, the performances of components and systems, and radiation and water chemistry have been carried out. 5 MWe was sent to grid system for the first time on July 29, 1978. The commercial operation of the plant was licenced by the Government on March 30, 1979. The conceptual design of the 600 MWe demonstration plant was finished in early 1979, and the detailed design is to be carried out in 1979 and 1980. The main design principle was incorporated in the conceptual design, but some modifications are to be made to reduce the power cost and to facilitate the easy maintenance. (Kako, I.)

Design and fabrication of fuel for the prototype heavy water reactor Fugen

International Nuclear Information System (INIS)

Hasumi, Takashi; Yamanaka, Ryozi; Osawa, Masahide; Asami, Tomohiro; Kaziyama, Takashi

1983-01-01

For the advanced thermal reactor Fugen, 224 fuel assemblies were charged as the initial charge fuel, of which 96 were uranium-plutonium mixed oxide fuel, and 128 were uranium dioxide fuel. Since the full scale operation was started in March, 1979, fuel exchange was carried out five times, and 240 fuel assemblies were taken out, but fuel breaking was never found, and the fuel for Fugen has shown good result. For 16 mixed oxide fuel assemblies for the third exchange and thereafter, the domestically produced plutonium extracted in the Tokai reprocessing plant has been used, and for 15 UO 2 fuel assemblies for the fifth exchange, the enriched uranium produced in the Ningyo Pass plant was used. These fuels are burning in the core without causing trouble. The course of the development of the fuel is described as follows: trial manufacture, evaluation test outside the core, heat transferring flow characteristic test, irradiation test, design of fuel elements and fuel assemblies, production of fuel and quality assurance, and results of production and use. (Kako, I.)

On three-dimensional nuclear thermo-hydraulic computation techniques for ATR

International Nuclear Information System (INIS)

1997-08-01

The three-dimensional computation code for nuclear thermo-hydraulic combination core LAYMON-2A is used for the calculation of the power distribution and the control rod reactivity value of the ATR. This code possesses various functions which are required for planning the core operation such as the search function for critical boric acid concentration, and can do various simulation calculations such as core burning calculation. Further, the three-dimensional analysis code for xenon dynamic characteristics in the core LAYMON-2C, in which the dynamic characteristic equation of xenon-samarium was incorporated into the LAYMON-2A code can take the change with time lapse of xenon-samarium concentration accompanying the change of power level and power distribution into account, and it is used for the analysis of the spatial vibration characteristics of power and the regional power control characteristics due to xenon in the core. As to the LAYMON-2A, the computation flow, power distribution and thermo-hydraulic computation models, and critical search function are explained. As to the LAYMON-2C, the computation flow is described. The comparison of the calculated values by using the LAYMON-2A code and the operation data of the Fugen is reported. (K.I.)

Experiences of development of IT support systems for decommissioning of Fugen NPS

International Nuclear Information System (INIS)

Iguchi, Y.; Kato, Y.; Yanagihara, I.S.; Tezuka, M.; Koda, Y.

2017-01-01

Fugen (ATR: Advanced Thermal Reactor) is a 165 MWe, heavy water moderated, light water cooled, pressure-tube type reactor. After ca. 25 year operation, Fugen started decommissioning activities after the final shutdown in 2003 and the decommissioning project will lasts at least until 2034. It is important to execute the decommissioning economically and rationally. Therefore, a systems engineering approach is necessary in order to optimize the workload, exposure dose, waste mass and cost by selecting appropriate dismantling plan at the planning stage of the decommissioning. Because the nuclear facilities have a large number of components and structures, it is necessary to evaluate the process effectively. For this reason, in order to make an efficient decommissioning plan, we have developed an IT support system for decommissioning by adopting new technologies such as 3-dimensional computer aided design (3D-CAD) and virtual reality (VR) named DEXUS. After the approval of the decommissioning plan by the regulatory authority, we have started the decommissioning activities from dismantlement of turbine systems (which is slightly contaminated with radioactive material), designing remote-control systems of reactor removal by laser cutting, development of radioactive waste control system and application including the clearance method, etc. By using IT support systems for these activities more than 10 years, we have been accumulating useful experiences and lessons-learned. The most important thing is the financial optimization. The decommissioning of a plant is non-profit and one-time-only activities, then the plan should be rationalized and the budget should be minimized as much as possible. In other words, we should consider cost-benefit for the computer systems development and implementation. A graded approach should be introduced in this point of view. For example, 3D-CAD data is very useful for the planning of dismantling of highly radioactive materials such as reactor

Experience with, and programme of, FBR and HWR development in Japan

International Nuclear Information System (INIS)

Iida, M.; Sawai, S.; Nomoto, S.

1983-01-01

Nuclear power generation in Japan is moving forward on the long-term development programme of nuclear power from the LWR to the FBR, essentially in the same way as in other advanced nuclear countries. In this development programme the unique HWR is also included; it can use plutonium produced in LWRs together with depleted uranium before the introduction of commercial FBRs. This report describes the status of the FBR and HWR development project being carried out by the Power Reactor and Nuclear Fuel Development Corporation (PNC) based upon the Long-Term Programme on Research, Development and Utilization of Nuclear Energy in Japan. Operational experience and technical results are shown for the experimental fast reactor JOYO (100 MW(th)), which reached initial criticality in 1977. The status of the 280 MW(e) prototype reactor MONJU, under construction as of 1982, is described. The conceptual design of the subsequent 1000 MW(e) demonstration plant is outlined, as is additional future planning. Research and development results, mainly carried out at Oarai Engineering Center of PNC, are shown. The 165 MW(e) prototype FUGEN is a heavy-water-moderated, boiling-light-water-cooled, pressure-tube-type reactor which uses plutonium mixed-oxide fuel. This report describes the relationship of the fuel cycle to the HWR in Japan and also discusses the operational experience of the prototype FUGEN, which has operated since 1979. Also described is the design of the 600 MW(e) demonstration plant and the programme of related research and development. (author)

Activities of Knowledge Management for Decommissioning of FUGEN Nuclear Power Station

International Nuclear Information System (INIS)

Tezuka, M.; Iguchi, Y.; Koda, Y.; Kato, Y.; Yanagihara, S.

2016-01-01

Full text: The Fugen nuclear power station is a heavy–water moderated, light–water cooled, pressure– tube type reactor. After ca. 25 year operation, Fugen started decommissioning activities after the final shutdown in 2003 and the decommissioning project will last at least until 2034. In this situation, as the transfer of knowledge and education to the next generation is a crucial issue, integration and implementation of a system for knowledge management is necessary to solve it. For this purpose, a total of knowledge management system (KMS) for decommissioning was proposed. In this system, we have to arrange, organize and systematize the data and information of the plant design, maintenance history, waste management records etc. The collected data, information and records should be organized by computer support system, e.g., data base system, as well as advanced information technologies such as 3D-CAD (Computer Aided Design), VR (Virtual Reality) and AR (Augmented Reality). It will become a base of the explicit knowledge. Moreover, measures for extracting tacit knowledge from retiring employees are necessary. The experience of the retirees should be documented as much as possible through effective questionnaire or interview process. The integrated knowledge mentioned above should be used for the planning, implementation of dismantlement or education for the future generation. (author

77 FR 67557 - Special Conditions: ATR-GIE Avions de Transport Regional, Models ATR42-500 and ATR72-212A...

Science.gov (United States)

2012-11-13

..., Models ATR42-500 and ATR72-212A Airplanes; Aircraft Electronic System Security Protection From... exploited by unauthorized access to airplane systems, data buses, and servers. Therefore, these special... ATR42-500 and ATR72-212A airplanes. 1. Airplane Electronic System Security Protection from Unauthorized...

Development of on-line core performance evaluation system for 'FUGEN'

International Nuclear Information System (INIS)

Natori, Hisahide; Kaneto, Kunikazu; Oteru, Shigeru.

1982-01-01

An on-line core performance evaluation system ATROPOS has been developed in order to carry out safe and efficient reactor operation of ''FUGEN''(a heavy water moderated, boiling light water cooled, pressure tube type reactor). This system offers detailed and useful information on such items of core performance as core thermal power, power distribution and thermal operation limits. The power distribution is calculated first by using a three-dimensional nodal coupling model, employing such process data as control rod position and 10 B concentration in the D 2 O moderator. Then the calculated power distribution is corrected by local power monitor readings. An axial one-dimensional nodal coupling model, which considers radial power distribution, and a localized three-dimensional nodal coupling model are used to predict the core thermal power and the power distribution for the region surrounding the control rods respectively, within a short time in advance of control rod operation. The methods employed in this system are verified by comparison with start-up test data from the FUGEN initial core. The estimated power distribution and channel flow agree with values measured by the power calibration monitor and with channel flow converted from measured values of pressure drop, within 3 and 5% respectively, in their root mean square values. The difference in core thermal power between the predicted value and the value measured by the total power monitor is about 1% for control rod operation. (author)

Heavy water upgrading system in the Fugen heavy water reactor

International Nuclear Information System (INIS)

Matsushita, T.; Susaki, S.

1980-01-01

The heavy water upgrading system, which is installed in the Fugen heavy water reactor (HWR) was designed to reuse degraded heavy water generated from the deuteration-dedeuteration of resin in the ion exchange column of the moderator purification system. The electrolysis method has been applied in this system on the basis of the predicted generation rate and concentration of degraded heavy water. The structural feature of the electrolytic cell is that it consists of dual cylindrical electrodes, instead of a diaphragm as in the case of conventional water electrolysis. 2 refs

Simulated Performance of the Integrated Passive Neutron Albedo Reactivity and Self-Interrogation Neutron Resonance Densitometry Detector Designed for Spent Fuel Measurement at the Fugen Reactor in Japan

Energy Technology Data Exchange (ETDEWEB)

Ulrich, Timothy J. II [Los Alamos National Laboratory; Lafleur, Adrienne M. [Los Alamos National Laboratory; Menlove, Howard O. [Los Alamos National Laboratory; Swinhoe, Martyn T. [Los Alamos National Laboratory; Tobin, Stephen J. [Los Alamos National Laboratory; Seya, Michio [Los Alamos National Laboratory; Bolind, Alan M. [Los Alamos National Laboratory

2012-07-16

An integrated nondestructive assay instrument, which combined the Passive Neutron Albedo Reactivity (PNAR) and the Self-Interrogation Neutron Resonance Densitometry (SINRD) techniques, is the research focus for a collaborative effort between Los Alamos National Laboratory (LANL) and the Japanese Atomic Energy Agency as part of the Next Generation Safeguard Initiative. We will quantify the anticipated performance of this experimental system in two physical environments: (1) At LANL we will measure fresh Low Enriched Uranium (LEU) assemblies for which the average enrichment can be varied from 0.2% to 3.2% and for which Gd laced rods will be included. (2) At Fugen we will measure spent Mixed Oxide (MOX-B) and LEU spent fuel assemblies from the heavy water moderated Fugen reactor. The MOX-B assemblies will vary in burnup from {approx}3 GWd/tHM to {approx}20 GWd/tHM while the LEU assemblies ({approx}1.9% initial enrichment) will vary from {approx}2 GWd/tHM to {approx}7 GWd/tHM. The estimated count rates will be calculated using MCNPX. These preliminary results will help the finalization of the hardware design and also serve a guide for the experiment. The hardware of the detector is expected to be fabricated in 2012 with measurements expected to take place in 2012 and 2013. This work is supported by the Next Generation Safeguards Initiative, Office of Nuclear Safeguards and Security, National Nuclear Security Administration.

ATR performance modeling concepts

Science.gov (United States)

Ross, Timothy D.; Baker, Hyatt B.; Nolan, Adam R.; McGinnis, Ryan E.; Paulson, Christopher R.

2016-05-01

Performance models are needed for automatic target recognition (ATR) development and use. ATRs consume sensor data and produce decisions about the scene observed. ATR performance models (APMs) on the other hand consume operating conditions (OCs) and produce probabilities about what the ATR will produce. APMs are needed for many modeling roles of many kinds of ATRs (each with different sensing modality and exploitation functionality combinations); moreover, there are different approaches to constructing the APMs. Therefore, although many APMs have been developed, there is rarely one that fits a particular need. Clarified APM concepts may allow us to recognize new uses of existing APMs and identify new APM technologies and components that better support coverage of the needed APMs. The concepts begin with thinking of ATRs as mapping OCs of the real scene (including the sensor data) to reports. An APM is then a mapping from explicit quantized OCs (represented with less resolution than the real OCs) and latent OC distributions to report distributions. The roles of APMs can be distinguished by the explicit OCs they consume. APMs used in simulations consume the true state that the ATR is attempting to report. APMs used online with the exploitation consume the sensor signal and derivatives, such as match scores. APMs used in sensor management consume neither of those, but estimate performance from other OCs. This paper will summarize the major building blocks for APMs, including knowledge sources, OC models, look-up tables, analytical and learned mappings, and tools for signal synthesis and exploitation.

Development of data processing system for the start-up test of FUGEN

International Nuclear Information System (INIS)

Nakajima, Ichiro; Kato, Hidemasa

1981-01-01

The data processing in the start-up test of conventional reactors has been carried out by recording data with transient phenomena recorders (e.g. electromagnetic oscillographs) or analog data recorders. On the other hand, the rapid works for detailed comparison and investigation between the test data and the analyzed results have been indispensable because ''Fugen'' is a new type of reactor, for which the results in conventional reactors did not necessarily serve as reference. Therefore, in the start-up test of the ''Fugen'' plant, the test data processing and the forecast analysis were performed by installing on the site a mini-computer capable of independently processing the test data and a terminal equipment connected to a large computer with a special communication line. As soon as the testing was completed, the comparison of the test data with the forecast analysis was presented on a graphic display (CRT), and the analysis was modified until significant differences did not appear between the test data and the analyzed data. In this paper, the system hardware and software are described, and two functions of forecast analysis and test data processing are explained. The time required for printing-out or graphic display from inputting 600 kB analysis code data using the terminal equipment was 10 to 30 minutes, and the evaluation and investigation for the test results were able to be immediately achieved by the data processing using the mini-computer. This is one of the factors to carry out the start-up test satisfactorily together with the forecast analysis works. (Wakatsuki, Y.)

Design, manufacture and installation of measuring and control equipments for the advanced thermal prototype reactor 'Fugen'

International Nuclear Information System (INIS)

Hirota, Shigeo; Kawabata, Yoshinori

1979-01-01

The advanced thermal prototype reactor ''Fugen'' attained the criticality on March 20, 1978, and 100% output operation on November 13, 1978. On March 20, 1979, it passed the final inspection, and since then, it has continued the smooth operation up to now. The measuring and control equipments are provided for grasping the operational conditions of the plant and operating it safely and efficiently. At the time of designing, manufacturing and installing the measuring and control equipments for Fugen, it was required to clarify the requirements of the plant design, to secure the sufficient functions, and to improve the operational process, maintainability and the reliability and accuracy of the equipments. Many design guidelines and criteria were decided in order to coordinate the conditions among five manufacturers and give the unified state as one plant. The outline of the instrumentations for neutrons, radiation monitoring and process data, the control systems for reactivity, reactor output, pressure and water supply, the safety protection system, and the process computer are described. Finally, the installations and tests of the measuring and control equipments are explained. The aseismatic capability of the equipments was confirmed. (Kako, I.)

Development of long-life neutron detectors for the prototype heavy water reactor 'Fugen'

International Nuclear Information System (INIS)

Ohteru, Shigeru; Shirayama, Shimpey.

1981-01-01

The development of long-life neutron detectors as the flux monitors for the prototype heavy water reactor has been made. Three kinds of neutron monitors, namely start-up monitor (SUM), power up monitor (PUM) and local power monitor (LPM), are provided. The LPM consists of 4 ion chamber type neutron detectors and a guide tube of power calibration monitor (PCM). This is useful for reactor control and fuel soundness monitor. The improvement of the neutron detectors was made for the operation under high neutron flux and gamma-ray heating. For the long-life operation, U-234 was mixed into U-235 for the conversion in the detectors. The ratio of U-234 to U-235 is 3 to 1. The PCM is also an ion chamber type detector with U-235. The mixing ratio of U-234 to U-235 was determined by a test with the JMTR. The characteristic performance was also investigated by the JMTR. After the completion of Fugen, various tests on the long-life detectors were performed with Fugen. It was hard to test the output linearity of the detectors with a large scale reactor. Therefore, it was tested that the operation range of the detectors is within the linear region of detector output. The voltage-current characteristics and the correlation of output current and saturation current were measured. The variation of the neutron sensitivity of the detectors with the cumulative dose was also studied. (Kato, T.)

Function of the ATR N-terminal domain revealed by an ATM/ATR chimera

International Nuclear Information System (INIS)

Chen Xinping; Zhao Runxiang; Glick, Gloria G.; Cortez, David

2007-01-01

The ATM and ATR kinases function at the apex of checkpoint signaling pathways. These kinases share significant sequence similarity, phosphorylate many of the same substrates, and have overlapping roles in initiating cell cycle checkpoints. However, they sense DNA damage through distinct mechanisms. ATR primarily senses single stranded DNA (ssDNA) through its interaction with ATRIP, and ATM senses double strand breaks through its interaction with Nbs1. We determined that the N-terminus of ATR contains a domain that binds ATRIP. Attaching this domain to ATM allowed the fusion protein (ATM*) to bind ATRIP and associate with RPA-coated ssDNA. ATM* also gained the ability to localize efficiently to stalled replication forks as well as double strand breaks. Despite having normal kinase activity when tested in vitro and being phosphorylated on S1981 in vivo, ATM* is defective in checkpoint signaling and does not complement cellular deficiencies in either ATM or ATR. These data indicate that the N-terminus of ATR is sufficient to bind ATRIP and to promote localization to sites of replication stress

Outline of design, manufacturing and installation experience of pressure vessel structure for the prototype heavy water moderated boiling light water cooled reactor 'FUGEN'

International Nuclear Information System (INIS)

Shibato, Eizo; Oguchi, Isao; Kishi, Toshikazu; Kitagawa, Yuji

1977-01-01

After component installation completed in June 1977 and various functional tests to be conducted later, the prototype heavy water moderated, boiling light water cooled reactor ''FUGEN'' is scheduled to reach first criticality in March 1978. Since the pressure vessel of ''FUGEN'' is completely different from that of a light water reactor in structure and materials, through research and development work was carried out prior to fabrication and construction. Based on these studies, installation of the actual pressure vessel was completed. Functional tests are now under way. This article describes examples in which our research and development results are reflected on design, manufacture, and installation of the pressure vessel. Also it introduces noteworthy achievements relevant to production techniques in manufacture and installation. (auth.)

Study on applicability of evaluation model of manpower needs for dismantling of equipments in FUGEN-1. Dismantling process in 3rd/4th feedwater heater room

International Nuclear Information System (INIS)

Shibahara, Yuji; Izumi, Masanori; Nanko, Takashi; Tachibana, Mitsuo; Ishigami, Tsutomu

2010-10-01

Manpower needs for the dismantling process on the dismantling of equipments in FUGEN 3rd/4th feedwater heater room was calculated with the management data evaluation system (PRODIA Code), and it was inspected whether the conventional evaluation model had applicability for FUGEN or not. It was confirmed that the conventional evaluation model for feedwater heater had no applicability. In comparison of the calculated value with the actual data, we found two difference: 1) the calculated value were significantly larger than the actual data, 2) the actual data for the dismantling of 3rd feedwater heater was twice larger than that of 4th feedwater heater, though these equipments were almost same weight. It was found that these were brought 1) by the difference in the work descriptions of dismantling between JPDR and FUGEN, and 2) by that in the cutting number between 3rd feedwater heater and 4th one. The manpower needs for the dismantling of both feedwater heaters were calculated with a new calculation equation reflecting the descriptions of dismantling, and it was found that these results showed the good agreement with the actual data. (author)

A state-of-the art on decommissioning of nuclear facilities in Japan

International Nuclear Information System (INIS)

Park, Seung Kook; Kim, Hee Reyoung; Chung, Un Soo; Jung, Ki Jung

2002-05-01

While proceeding the KRR-1 and 2 decommissioning project, we are carried out study for the state of the art on decommissioning of nuclear facilities in Japan. Also, we are studied for the research reactors and commercial power plant that has the object of decommissioning, and for the government and the organization related on decommissioning operation. We are investigated for decommissioning activities of nuclear facilities achieved by JAERI, and collected the information and data for decommissioning techniques and computational system through the JPDR(Japan Power Demonstration Reactor) decommissioning activities. Such techniques are applying for Tokai Power Station began the decommissioning project from last year, and for Fugen Nuclear Power Station to be planned the decommissioning from 2003. Recent techniques for decommissioning was acquired by direct contact. The status of the treatment for decommissioning waste and the disposal facility for the very low-level radioactive concrete wastes was grasped

Radiation exposure of the employes in fiscal 1980 at reactor facilities for testing and research and under development

International Nuclear Information System (INIS)

1982-01-01

The owners of reactors are obligated by the law for the regulation of reactors, etc. to keep the radiation exposure dose of their employes below the permissible level. In fiscal 1980 (from April to March), the exposure dose of employes was largely below the permissible level. Based on the reports made by the owners in accordance with the law, the following data are presented in tables for the whole year and the respective quarters: in the research institutions including Japan Atomic Energy Research Institute (JAERI), Power Reactor and Nuclear Fuel Development Corporation (PNC) and educational institutions, the exposure dose distribution of employes; in the Tokai Research Establishment and Oarai Research Establishment, JAERI, and the Oarai Engineering Center and ATR ''Fugen'' Power Station, PNC, the exposure dose distribution, total exposure dose and average exposure dose of employes and outside workers. (J.P.N.)

Accidents and failures in reactor facilities for test and research and reactor facilities in the stage of research and development in fiscal year 1987

International Nuclear Information System (INIS)

1988-01-01

The number of accidents and failures reported in fiscal year 1987 in conformity with the law on the regulation of nuclear reactors and others was three. One case occurred during operation, and two cases occurred in shutdown state. One case was caused by improper construction management, and two cases were due to improper maintenance management. The effect of radioactivity to the surrounding environment of reactor facilities due to these accidents and failures did not arise. These occurred in the NSRR of Japan Atomic Energy Research Institute (Tokai), the experimental FBR Joyo and the ATR Fugen Power Station of Power Reactor and Nuclear Fuel Development Corp. In addition to these, the light troubles reported on the basis of the notice from the director of Science and Technology Agency dated September 1, 1981, were three cases. (K.I.)

ATR neutron spectral characterization

Energy Technology Data Exchange (ETDEWEB)

Rogers, J.W.; Anderl, R.A.

1995-11-01

The Advanced Test Reactor (ATR) at INEL provides intense neutron fields for irradiation-effects testing of reactor material samples, for production of radionuclides used in industrial and medical applications, and for scientific research. Characterization of the neutron environments in the irradiation locations of the ATR has been done by means of neutronics calculations and by means of neutron dosimetry based on the use of neutron activation monitors that are placed in the various irradiation locations. The primary purpose of this report is to present the results of an extensive characterization of several ATR irradiation locations based on neutron dosimetry measurements and on least-squares-adjustment analyses that utilize both neutron dosimetry measurements and neutronics calculations. This report builds upon the previous publications, especially the reference 4 paper. Section 2 provides a brief description of the ATR and it tabulates neutron spectral information for typical irradiation locations, as derived from the more historical neutron dosimetry measurements. Relevant details that pertain to the multigroup neutron spectral characterization are covered in section 3. This discussion includes a presentation on the dosimeter irradiation and analyses and a development of the least-squares adjustment methodology, along with a summary of the results of these analyses. Spectrum-averaged cross sections for neutron monitoring and for displacement-damage prediction in Fe, Cr, and Ni are given in section 4. In addition, section4 includes estimates of damage generation rates for these materials in selected ATR irradiation locations. In section 5, the authors present a brief discussion of the most significant conclusions of this work and comment on its relevance to the present ATR core configuration. Finally, detailed numerical and graphical results for the spectrum-characterization analyses in each irradiation location are provided in the Appendix.

Development of decommissioning engineering support system for fugen. Development of support system during actual dismantlement works

International Nuclear Information System (INIS)

Masanori Izumi; Yukihiro Iguchi; Yoshiki Kannehira

2005-01-01

The Advanced Thermal Reactor, Fugen Nuclear Power Station was permanently shut down in March 2003, and is now preparing for decommissioning. We have been developing Decommissioning Engineering Support System (DEXUS) aimed at planning optimal dismantlement process and carrying out dismantlement work safely and precisely. DEXUS consists of 'decommissioning planning support system' and 'dismantling support system'. The dismantling support system is developed aiming at using during actual dismantling work. It consists of three subsystems such as 'Worksite Visualization System', 'Dismantling Data Collection System' and 'Generated Waste Management System'. 'Worksite Visualization System' is a support system designed to provide the necessary information to workers during actual dismantlement works. And this system adopts AR (Augmented Reality) technology, overlapping calculation information into real world. 'Dismantling Data Collection System' is to collect necessary data for improving accuracy of decommissioning planning by evaluating work content and worker equipage, work time for dismantlement works. 'Generated Waste Management system' is a system recording necessary information by attaching the barcode to dismantled wastes or the containers. We can get the information of generated waste by recording generation place, generated time, treatment method and the contents. These subsystems enable to carry out reasonable and safe decommissioning of Fugen. In addition, we expect that those systems will be used for decommissioning of other nuclear facilities in the future. (authors)

Development of acoustic leak detection and localization methods for inlet piping of fugen nuclear power plant

International Nuclear Information System (INIS)

Shimanskiy, Sergey; Iijima, Takashi; Naoi, Yosuke

2004-01-01

The development work carried out on Fugen NPP is focused on detection of a small leakage on the reactor's inlet feeder pipes at an early stage by an acoustic leak detection method with usage of high-temperature resistant microphones. Specifically, the leak rate of 0.046m 3 /h has been chosen as a target detection capability for this system. A cross-correlation technique has been studied for leak detection under low signal-noise ratios. The study shows that the sound diffusion on piping causes distortion of leak signals that results in their low correlation. A leak-location estimator and multi-channel correlation value, associated with estimated leak position, have been employed to detect such low-correlated leak signals. A method based on cross-correlation of signal spectral components has been proposed to deal with non-stationary leak signals. Joint-Time-Frequency-Analysis has been applied to analyze such signals, whilst a Wavelet decomposition technique has been used to extract their short-term spectral fluctuations. Since the spectral components are less affected by signal distortion, they provide higher correlation value and can be applied for leak detection under lower signal-noise ratios. The possibility of detecting and locating a small leakage by the methods proposed has been demonstrated by a number of simulation tests conducted on the Fugen NPP site. (author)

Looking back on safety management in construction of advanced thermal nuclear power plant 'Fugen'

International Nuclear Information System (INIS)

Emori, Kengo

1979-01-01

The safety management of the advanced thermal nuclear power plant ''Fugen'' during the period from the preparation of plant construction in October, 1970, to the full power operation in March, 1979, is looked back and explained. Any large human and material accidents did not occur during the long construction time. The total numbers of persons and hours were 1.397 x 10 6 workers and 11.55 x 10 6 hours, respectively. The number of labor accidents was twenty with no dead person, the number of loss days was 645 days, the number of accident rate was 1.73, the intensity rate was 0.06, and the mean rate of labor accidents per year per 1000 workers was 6.4. The radiation exposure dose was 65.27 man-rem for the managed 1804 workers in total, during the testing and operating periods. These data show that the safety management for ''Fugen'' is very excellent, considering the following special features: 1) there were many works which were carried out for the first time, 2) the construction of the plant was conducted by five contractors taking partial charge, there were many kinds of construction works, the construction and testing periods were long, and the workers had to go to the site from Tsuruga city by car and bus. The organization of preventing disasters, the concrete implementation items for safety management, including the planning of activities, various meetings, patrol, education and training, the honoring system, the prevention of traffic accident and so on, and the results of actual safety management are explained with the reflection. (Nakai, Y.)

Cytoplasmic ATR Activation Promotes Vaccinia Virus Genome Replication

Directory of Open Access Journals (Sweden)

Antonio Postigo

2017-05-01

Full Text Available In contrast to most DNA viruses, poxviruses replicate their genomes in the cytoplasm without host involvement. We find that vaccinia virus induces cytoplasmic activation of ATR early during infection, before genome uncoating, which is unexpected because ATR plays a fundamental nuclear role in maintaining host genome integrity. ATR, RPA, INTS7, and Chk1 are recruited to cytoplasmic DNA viral factories, suggesting canonical ATR pathway activation. Consistent with this, pharmacological and RNAi-mediated inhibition of canonical ATR signaling suppresses genome replication. RPA and the sliding clamp PCNA interact with the viral polymerase E9 and are required for DNA replication. Moreover, the ATR activator TOPBP1 promotes genome replication and associates with the viral replisome component H5. Our study suggests that, in contrast to long-held beliefs, vaccinia recruits conserved components of the eukaryote DNA replication and repair machinery to amplify its genome in the host cytoplasm.

Determination of the environmental effect for the sea salt aerosols corrosion (3)

International Nuclear Information System (INIS)

Nakayasu, Fumio; Umehara, Toshihiro; Kato, Akitoshi; Taniguchi, Akihide

2009-01-01

Fukui Prefecture presently hosts 15 reactor units, including PWRs, BWR, FBR (Monjyu) and Japanese ATR (Fugen), all of which are located along the Japan Sea. Under such a circumstange, corrosion due to sea salt aerosols is one of the major factors causing ageing degradation of nuclear power plants facilities. Many scientists have been engaged in research on the corrosion of structures due to sea salt aerosols. In pursuing sea salt aerosol-induced corrosion research, it was necessary to perform corrosion tests under a certain set of environmental conditions since corrosion of structures is highly sensitive to environmental factors. In this respect, we installed the outdoor exposure test facilities along the sea coast in Awara-city and inland in Fukui-city, Fukui Prefecture of Japan. The amounts of sea salt aerosol tend to be lower in the summer season and higher in the winter season. The difference between seasons is expected to relate to the wind speed, the sunshine time, the rain amount and others. We tried to use the multivariate analysis for finding some relations between sea salt aerosol amounts and environment factor. We found the clear relations. (author)

Safety significance of ATR passive safety response attributes

International Nuclear Information System (INIS)

Atkinson, S.A.

1990-01-01

The Advanced Test Reactor (ATR) at the Idaho National Engineering Laboratory was designed with some passive safety response attributes which contribute to the safety of the facility. The three passive safety attributes being evaluated in the paper are: 1) In-core and in-vessel natural convection cooling, 2) a passive heat sink capability of the ATR primary coolant system (PCS) for the transfer of decay power from the uninsulated piping to the confinement, and 3) gravity feed of emergency coolant makeup. The safety significance of the ATR passive safety response attributes is that the reactor can passively respond to most transients, given a reactor scram, to provide adequate decay power removal and a significant time for operator action should the normal active heat removal systems and their backup systems both fail. The ATR Interim Level 1 Probabilistic Risk Assessment (PRA) models and results were used to evaluate the significance to ATR fuel damage frequency (or probability) of the above three passive response attributes. The results of the evaluation indicate that the first attribute is a major safety characteristic of the ATR. The second attribute has a noticeable but only minor safety significance. The third attribute has no significant influence on the ATR firewater injection system (emergency coolant system)

ATR-dependent bystander effects in non-targeted cells

International Nuclear Information System (INIS)

Burdak-Rothkamm, S.

2007-01-01

Complete text of publication follows. Radiation induced non-targeted bystander effects have been reported for a range of endpoints including the induction of γH2AX foci which serve as a marker for DNA double strand breaks. We have recently reported the induction of γH2AX foci in non-targeted bystander cells up to 48 hours after irradiation and the involvement of reactive oxygen species (ROS) and TGF-beta 1 in the induction of γH2AX foci (Oncogene (2007) 26:993-1002). Here, we wanted to determine the role of the PI3-like kinases ATM, ATR and DNA-PK in DNA damage signalling in bystander cells. Conditioned medium from T98G cells irradiated with 2 Gy of X-rays was transferred onto non-irradiated cells that were subsequently analysed for the induction of γH2AX, ATR and 53BP1 foci as well as clonogenic survival. Irradiated T98G glioma cells generated signals that induced γH2AX and 53BP1 foci in cells treated with the conditioned medium from irradiated cells. These foci co-localised with ATR foci. Inhibition of ATM and DNA-PK could not suppress the induction of bystander γH2AX foci whereas the mutation of ATR in Seckel cells abrogated bystander foci induction. A restriction of bystander foci to the S-phase of the cell cycle both in T98G cells and in ATR- proficient fibroblasts was observed. These results identify ATR as a central player within the bystander signalling cascade leading to γH2AX and 53BP1 foci formation, and suggest a mechanism of DNA damage induction in non-targeted cells. Further investigations have shown decreased clonogenic cell survival in bystander T98G and ATR wild-type fibroblasts. ATR mutated Seckel cells and also ATM-/- fibroblasts were resistant to this effect suggesting a role for both ATR and ATM in the bystander signalling cascade with regard to cell survival. Taken together, these observations support a hypothesis of DNA damage-induced accumulation of stalled replication forks in bystander cells which are subsequently processed by

Focusing and matching properties of the ATR transfer line

International Nuclear Information System (INIS)

Tsoupas, N.; Fischer, W.; Kewisch, J.; MacKay, W.W.; Peggs, S.; Pilat, F.; Tepikian, S.; Wei, J.

1997-01-01

The AGS to RHIC (AtR) beam transfer line has been constructed and will be used to transfer beam bunches from the AGS machine into the RHIC machine which is presently under construction at BNL. The original design of the AtR line has been modified. This article will present the optics of the various sections of the existing AtR beam line, as well as the matching capabilities of the AtR line to the RHIC machine

ATR Spent Fuel Options Study

International Nuclear Information System (INIS)

Connolly, Michael James; Bean, Thomas E.; Brower, Jeffrey O.; Luke, Dale E.; Patterson, M. W.; Robb, Alan K.; Sindelar, Robert; Smith, Rebecca E.; Tonc, Vincent F.; Tripp, Julia L.; Winston, Philip L.

2017-01-01

The Advanced Test Reactor (ATR) is a materials and fuels test nuclear reactor that performs irradiation services for the U.S. Department of Energy (DOE) Office of Nuclear Energy (NE), Naval Reactors, the National Nuclear Security Administration (NNSA), and other research programs. ATR achieved initial criticality in 1967 and is expected to operate in support of needed missions until the year 2050 or beyond. It is anticipated that ATR will generate approximately 105 spent nuclear fuel (SNF) elements per year through the year 2050. Idaho National Laboratory (INL) currently stores 2,008 ATR SNF elements in dry storage, 976 in wet storage, and expects to have 1,000 elements in wet storage before January 2017. A capability gap exists at INL for long-term (greater than the year 2050) management, in compliance with the Idaho Settlement Agreement (ISA), of ATR SNF until a monitored retrievable geological repository is open. INL has significant wet and dry storage capabilities that are owned by the DOE Office of Environmental Management (EM) and operated and managed by Fluor Idaho, which include the Idaho Nuclear Technology and Engineering Center's (INTEC's) CPP-666, CPP-749, and CPP-603. In addition, INL has other capabilities owned by DOE-NE and operated and managed by Battelle Energy Alliance, LLC (BEA), which are located at the Materials and Fuel Complex (MFC). Additional storage capabilities are located on the INL Site at the Naval Reactors Facility (NRF). Current INL SNF management planning, as defined in the Fluor Idaho contract, shows INTEC dry fuel storage, which is currently used for ATR SNF, will be nearly full after transfer of an additional 1,000 ATR SNF from wet storage. DOE-NE tasked BEA with identifying and analyzing options that have the potential to fulfill this capability gap. BEA assembled a team comprised of SNF management experts from Fluor Idaho, Savannah River Site (SRS), INL/BEA, and the MITRE Corp with an objective of developing and analyzing

ATR Spent Fuel Options Study

Energy Technology Data Exchange (ETDEWEB)

Connolly, Michael James [Idaho National Lab. (INL), Idaho Falls, ID (United States); Bean, Thomas E. [Idaho National Lab. (INL), Idaho Falls, ID (United States); Brower, Jeffrey O. [Idaho National Lab. (INL), Idaho Falls, ID (United States); Luke, Dale E. [Idaho National Lab. (INL), Idaho Falls, ID (United States); Patterson, M. W. [Idaho National Lab. (INL), Idaho Falls, ID (United States); Robb, Alan K. [Idaho National Lab. (INL), Idaho Falls, ID (United States); Sindelar, Robert [Idaho National Lab. (INL), Idaho Falls, ID (United States); Smith, Rebecca E. [Idaho National Lab. (INL), Idaho Falls, ID (United States); Tonc, Vincent F. [Idaho National Lab. (INL), Idaho Falls, ID (United States); Tripp, Julia L. [Idaho National Lab. (INL), Idaho Falls, ID (United States); Winston, Philip L. [Idaho National Lab. (INL), Idaho Falls, ID (United States)

2017-01-01

The Advanced Test Reactor (ATR) is a materials and fuels test nuclear reactor that performs irradiation services for the U.S. Department of Energy (DOE) Office of Nuclear Energy (NE), Naval Reactors, the National Nuclear Security Administration (NNSA), and other research programs. ATR achieved initial criticality in 1967 and is expected to operate in support of needed missions until the year 2050 or beyond. It is anticipated that ATR will generate approximately 105 spent nuclear fuel (SNF) elements per year through the year 2050. Idaho National Laboratory (INL) currently stores 2,008 ATR SNF elements in dry storage, 976 in wet storage, and expects to have 1,000 elements in wet storage before January 2017. A capability gap exists at INL for long-term (greater than the year 2050) management, in compliance with the Idaho Settlement Agreement (ISA), of ATR SNF until a monitored retrievable geological repository is open. INL has significant wet and dry storage capabilities that are owned by the DOE Office of Environmental Management (EM) and operated and managed by Fluor Idaho, which include the Idaho Nuclear Technology and Engineering Center’s (INTEC’s) CPP-666, CPP-749, and CPP-603. In addition, INL has other capabilities owned by DOE-NE and operated and managed by Battelle Energy Alliance, LLC (BEA), which are located at the Materials and Fuel Complex (MFC). Additional storage capabilities are located on the INL Site at the Naval Reactors Facility (NRF). Current INL SNF management planning, as defined in the Fluor Idaho contract, shows INTEC dry fuel storage, which is currently used for ATR SNF, will be nearly full after transfer of an additional 1,000 ATR SNF from wet storage. DOE-NE tasked BEA with identifying and analyzing options that have the potential to fulfill this capability gap. BEA assembled a team comprised of SNF management experts from Fluor Idaho, Savannah River Site (SRS), INL/BEA, and the MITRE Corp with an objective of developing and analyzing

FLUX SENSOR EVALUATIONS AT THE ATR CRITICAL FACILITY

International Nuclear Information System (INIS)

Unruh, Troy; Rempe, Joy; Nigg, David; Imel, George; Harris, Jason; Bonebrake, Eric

2010-01-01

The Advanced Test Reactor (ATR) and the ATR Critical (ATRC) facilities lack real-time methods for detecting thermal neutron flux and fission reaction rates for irradiation capsules. Direct measurements of the actual power deposited into a test are now possible without resorting to complicated correction factors. In addition, it is possible to directly measure minor actinide fission reaction rates and to provide time-dependent monitoring of the fission reaction rate or fast/thermal flux during transient testing. A joint Idaho State University/Idaho National Laboratory ATR National Scientific User Facility (ATR NSUF) project was recently initiated to evaluate new real-time state-of-the-art in-pile flux detection sensors. Initially, the project is comparing the accuracy, response time, and long duration performance of French Atomic Energy Commission (CEA)-developed miniature fission chambers, specialized self-powered neutron detectors (SPNDs) by the Argentinean National Energy Commission (CNEA), specially developed commercial SPNDs, and back-to-back fission (BTB) chambers developed by Argonne National Laboratory (ANL). As discussed in this paper, specialized fixturing and software was developed by INL to facilitate these joint ISU/INL evaluations. Calculations were performed by ISU to assess the performance of and reduce uncertainties in flux detection sensors and compare data obtained from these sensors with existing integral methods employed at the ATRC. Ultimately, project results will be used to select the detector that can provide the best online regional ATRC power measurement. It is anticipated that project results may offer the potential to increase the ATRC's current power limit and its ability to perform low-level irradiation experiments. In addition, results from this effort will provide insights about the viability of using these detectors in the ATR. Hence, this effort complements current activities to improve ATR software tools, computational protocols

ATR prohibits replication catastrophe by preventing global exhaustion of RPA.

Science.gov (United States)

Toledo, Luis Ignacio; Altmeyer, Matthias; Rask, Maj-Britt; Lukas, Claudia; Larsen, Dorthe Helena; Povlsen, Lou Klitgaard; Bekker-Jensen, Simon; Mailand, Niels; Bartek, Jiri; Lukas, Jiri

2013-11-21

ATR, activated by replication stress, protects replication forks locally and suppresses origin firing globally. Here, we show that these functions of ATR are mechanistically coupled. Although initially stable, stalled forks in ATR-deficient cells undergo nucleus-wide breakage after unscheduled origin firing generates an excess of single-stranded DNA that exhausts the nuclear pool of RPA. Partial reduction of RPA accelerated fork breakage, and forced elevation of RPA was sufficient to delay such "replication catastrophe" even in the absence of ATR activity. Conversely, unscheduled origin firing induced breakage of stalled forks even in cells with active ATR. Thus, ATR-mediated suppression of dormant origins shields active forks against irreversible breakage via preventing exhaustion of nuclear RPA. This study elucidates how replicating genomes avoid destabilizing DNA damage. Because cancer cells commonly feature intrinsically high replication stress, this study also provides a molecular rationale for their hypersensitivity to ATR inhibitors. Copyright © 2013 Elsevier Inc. All rights reserved.

On techniques of ATR lattice computation

International Nuclear Information System (INIS)

1997-08-01

Lattice computation is to compute the average nuclear constants of unit fuel lattice which are required for computing core nuclear characteristics such as core power distribution and reactivity characteristics. The main nuclear constants are infinite multiplying rate, neutron movement area, cross section for diffusion computation, local power distribution and isotope composition. As for the lattice computation code, WIMS-ATR is used, which is based on the WIMS-D code developed in U.K., and for the purpose of heightening the accuracy of analysis, which was improved by adding heavy water scattering cross section considering the temperature dependence by Honeck model. For the computation of the neutron absorption by control rods, LOIEL BLUE code is used. The extrapolation distance of neutron flux on control rod surfaces is computed by using THERMOS and DTF codes, and the lattice constants of adjoining lattices are computed by using the WIMS-ATR code. As for the WIMS-ATR code, the computation flow and nuclear data library, and as for the LOIEL BLUE code, the computation flow are explained. The local power distribution in fuel assemblies determined by the WIMS-ATR code was verified with the measured data, and the results are reported. (K.I.)

ATR Commissioning Software Task Force Report

Energy Technology Data Exchange (ETDEWEB)

D' Ottavio, Ted [Brookhaven National Lab. (BNL), Upton, NY (United States); Kewisch, Jorg [Brookhaven National Lab. (BNL), Upton, NY (United States); Saltmarsh, Chris [Brookhaven National Lab. (BNL), Upton, NY (United States); Sathe, Smita [Brookhaven National Lab. (BNL), Upton, NY (United States); Satogata, Todd [Brookhaven National Lab. (BNL), Upton, NY (United States); Shea, Don [Brookhaven National Lab. (BNL), Upton, NY (United States); Tepikian, Steve [Brookhaven National Lab. (BNL), Upton, NY (United States); Trahern, Garry [Brookhaven National Lab. (BNL), Upton, NY (United States)

1994-12-16

The Beam Injection Tests Software Task Force was charged with studying the software needed for the ATR tests, seen as a stepping stone or template for the larger scope of the full RHIC control system. This report outlines our avenues of exploration so far, presents the current analysis and implementation work in progress, and gives recommendations for the future on the ATR and longer time scales.

Present status and problems of nuclear power generation

International Nuclear Information System (INIS)

Harada, Hiroshi.

1984-01-01

The nuclear power generation in Japan began in 1963 with the successful power generation in the JPDR of the Japan Atomic Energy Research Institute, and since then, more than 20 years have elapsed. The Japan Atomic Power Co. started the operation of an imported Calder Hall type gas-cooled reactor with 166,000 kWe output in Tokai Nuclear Power Station in July, 1966. In 1983, the quantity of nuclear power generation was 113.1 billion kWh, which was equivalent to 21.4 % of the total power generation in Japan. As of April 1, 1984, 25 nuclear power plants with 18.28 million kW output were in operation, 12 plants of 11.8 million kW were under construction, and 7 plants of 6.05 million kW were in preparation phase. Besides, the ATR ''Fugen'' with 165,000 kW output has been in operation, and the FBR ''Monju'' with 280,000 kW output is under construction. The capacity ratio of Japanese nuclear power stations attained 71.5 % in 1983. According to the ''Long term energy demand and supply outlook'' revised in November, 1983, the nuclear power generation in 2000 will be about 62 million kW to cater for about 16 % of primary energy supply. The problems are the improvement of economy, the establishment of independent nuclear fuel cycle, the decommissioning of nuclear reactors and so on. (Kako, I.)

ATR confinement leakage determination

International Nuclear Information System (INIS)

Kuan, P.; Buescher, B.J.

1998-01-01

The air leakage rate from the Advanced Test Reactor (ATR) confinement is an important parameter in estimating hypothesized accidental releases of radiation to the environment. The leakage rate must be determined periodically to assure that the confinement has not degraded with time and such determination is one of the technical safety requirements of ATR operation. This paper reviews the methods of confinement leakage determination and presents an analysis of leakage determination under windy conditions, which can complicate the interpretation of the determined leakage rates. The paper also presents results of analyses of building air exchange under windy conditions. High wind can enhance air exchange and this could increase the release rates of radioisotopes following an accident

Atlantic Test Range (ATR)

Data.gov (United States)

Federal Laboratory Consortium — ATR controls fully-instrumented and integrated test ranges that provide full-service support for cradle-to-grave testing. Airspace and surface target areas are used...

Station power supply by residual steam of Fugen

Energy Technology Data Exchange (ETDEWEB)

Kamiya, Y.; Kato, H.; Hattori, S. (Power Reactor and Nuclear Fuel Development Corp., Tokyo (Japan))

1981-09-01

In the advanced thermal reactor ''Fugen'', when the sudden decrease of load more than 40% occurs due to the failure of power system, the turbine regulating valve is rapidly shut, and the reactor is brought to scrum. However, the operation of turbo-generators is continued with the residual steam in the reactor, and the power for inside the station is supplied for 30 sec by the limiting timer, then the power-generating plant is automatically stopped. The reasons why such design was adopted are to reduce manual operation at the time of emergency, to continue water supply for cooling the reactor and to maintain the water level in the steam drum, and to reduce steam release from the safety valve and the turbine bypass valve. The output-load unbalance relay prevents the everspeed of the turbo-generator when load decreased suddenly, but when the failure of power system is such that recovers automatically in course of time, it does not work. The calculation for estimating the dynamic characteristics at the time of the sole operation within the station is carried out by the analysis code FATRAC. The input conditions for the calculation and the results are reported. Also the dynamic characteristics were actually tested to confirm the set value of the limiting timer and the safe working of turbine and generator trips. The estimated and tested results were almost in agreement.

A mitosis-specific and R loop-driven ATR pathway promotes faithful chromosome segregation.

Science.gov (United States)

Kabeche, Lilian; Nguyen, Hai Dang; Buisson, Rémi; Zou, Lee

2018-01-05

The ataxia telangiectasia mutated and Rad3-related (ATR) kinase is crucial for DNA damage and replication stress responses. Here, we describe an unexpected role of ATR in mitosis. Acute inhibition or degradation of ATR in mitosis induces whole-chromosome missegregation. The effect of ATR ablation is not due to altered cyclin-dependent kinase 1 (CDK1) activity, DNA damage responses, or unscheduled DNA synthesis but to loss of an ATR function at centromeres. In mitosis, ATR localizes to centromeres through Aurora A-regulated association with centromere protein F (CENP-F), allowing ATR to engage replication protein A (RPA)-coated centromeric R loops. As ATR is activated at centromeres, it stimulates Aurora B through Chk1, preventing formation of lagging chromosomes. Thus, a mitosis-specific and R loop-driven ATR pathway acts at centromeres to promote faithful chromosome segregation, revealing functions of R loops and ATR in suppressing chromosome instability. Copyright © 2018, American Association for the Advancement of Science.

Safety significance of ATR [Advanced Test Reactor] passive safety response attributes

International Nuclear Information System (INIS)

Atkinson, S.A.

1989-01-01

The Advanced Test Reactor (ATR) at the Idaho National Engineering Laboratory was designed with some passive safety response attributes which contribute to the safety posture of the facility. The three passive safety attributes being evaluated in the paper are: (1) In-core and in-vessel natural convection cooling, (2) a passive heat sink capability of the ATR primary coolant system (PCS) for the transfer of decay power from the uninsulated piping to the confinement, and (3) gravity feed of emergency coolant makeup. The safety significance of the ATR passive safety response attributes is that the reactor can passively respond for most transients, given a reactor scram, to provide adequate decay power removal and a significant time for operator action should the normal active heat removal systems and their backup systems both fail. The ATR Interim Level 1 Probabilistic Risk Assessment (PRA) model ands results were used to evaluate the significance to ATR fuel damage frequency (or probability) of the above three passive response attributes. The results of the evaluation indicate that the first attribute is a major safety characteristic of the ATR. The second attribute has a noticeable but only minor safety significance. The third attribute has no significant influence on the ATR Level 1 PRA because of the diversity and redundancy of the ATR firewater injection system (emergency coolant system). 8 refs., 4 figs., 1 tab

Activation of the ATR kinase by the RPA-binding protein ETAA1

DEFF Research Database (Denmark)

Haahr, Peter; Hoffmann, Saskia; Tollenaere, Maxim A X

2016-01-01

Activation of the ATR kinase following perturbations to DNA replication relies on a complex mechanism involving ATR recruitment to RPA-coated single-stranded DNA via its binding partner ATRIP and stimulation of ATR kinase activity by TopBP1. Here, we discovered an independent ATR activation pathway...... in vertebrates, mediated by the uncharacterized protein ETAA1 (Ewing's tumour-associated antigen 1). Human ETAA1 accumulates at DNA damage sites via dual RPA-binding motifs and promotes replication fork progression and integrity, ATR signalling and cell survival after genotoxic insults. Mechanistically...

Assessment of COTS IR image simulation tools for ATR development

Science.gov (United States)

Seidel, Heiko; Stahl, Christoph; Bjerkeli, Frode; Skaaren-Fystro, Paal

2005-05-01

Following the tendency of increased use of imaging sensors in military aircraft, future fighter pilots will need onboard artificial intelligence e.g. ATR for aiding them in image interpretation and target designation. The European Aeronautic Defence and Space Company (EADS) in Germany has developed an advanced method for automatic target recognition (ATR) which is based on adaptive neural networks. This ATR method can assist the crew of military aircraft like the Eurofighter in sensor image monitoring and thereby reduce the workload in the cockpit and increase the mission efficiency. The EADS ATR approach can be adapted for imagery of visual, infrared and SAR sensors because of the training-based classifiers of the ATR method. For the optimal adaptation of these classifiers they have to be trained with appropriate and sufficient image data. The training images must show the target objects from different aspect angles, ranges, environmental conditions, etc. Incomplete training sets lead to a degradation of classifier performance. Additionally, ground truth information i.e. scenario conditions like class type and position of targets is necessary for the optimal adaptation of the ATR method. In Summer 2003, EADS started a cooperation with Kongsberg Defence & Aerospace (KDA) from Norway. The EADS/KDA approach is to provide additional image data sets for training-based ATR through IR image simulation. The joint study aims to investigate the benefits of enhancing incomplete training sets for classifier adaptation by simulated synthetic imagery. EADS/KDA identified the requirements of a commercial-off-the-shelf IR simulation tool capable of delivering appropriate synthetic imagery for ATR development. A market study of available IR simulation tools and suppliers was performed. After that the most promising tool was benchmarked according to several criteria e.g. thermal emission model, sensor model, targets model, non-radiometric image features etc., resulting in a

Requirement of ATR for maintenance of intestinal stem cells in aging Drosophila.

Science.gov (United States)

Park, Joung-Sun; Na, Hyun-Jin; Pyo, Jung-Hoon; Jeon, Ho-Jun; Kim, Young-Shin; Yoo, Mi-Ae

2015-05-01

The stem cell genomic stability forms the basis for robust tissue homeostasis, particularly in high-turnover tissues. For the genomic stability, DNA damage response (DDR) is essential. This study was focused on the role of two major DDR-related factors, ataxia telangiectasia-mutated (ATM) and ATM- and RAD3-related (ATR) kinases, in the maintenance of intestinal stem cells (ISCs) in the adultDrosophila midgut. We explored the role of ATM and ATR, utilizing immunostaining with an anti-pS/TQ antibody as an indicator of ATM/ATR activation, γ-irradiation as a DNA damage inducer, and the UAS/GAL4 system for cell type-specific knockdown of ATM, ATR, or both during adulthood. The results showed that the pS/TQ signals got stronger with age and after oxidative stress. The pS/TQ signals were found to be more dependent on ATR rather than on ATM in ISCs/enteroblasts (EBs). Furthermore, an ISC/EB-specific knockdown of ATR, ATM, or both decreased the number of ISCs and oxidative stress-induced ISC proliferation. The phenotypic changes that were caused by the ATR knockdown were more pronounced than those caused by the ATM knockdown; however, our data indicate that ATR and ATM are both needed for ISC maintenance and proliferation; ATR seems to play a bigger role than does ATM.

Reconstitution of RPA-covered single-stranded DNA-activated ATR-Chk1 signaling.

Science.gov (United States)

Choi, Jun-Hyuk; Lindsey-Boltz, Laura A; Kemp, Michael; Mason, Aaron C; Wold, Marc S; Sancar, Aziz

2010-08-03

ATR kinase is a critical upstream regulator of the checkpoint response to various forms of DNA damage. Previous studies have shown that ATR is recruited via its binding partner ATR-interacting protein (ATRIP) to replication protein A (RPA)-covered single-stranded DNA (RPA-ssDNA) generated at sites of DNA damage where ATR is then activated by TopBP1 to phosphorylate downstream targets including the Chk1 signal transducing kinase. However, this critical feature of the human ATR-initiated DNA damage checkpoint signaling has not been demonstrated in a defined system. Here we describe an in vitro checkpoint system in which RPA-ssDNA and TopBP1 are essential for phosphorylation of Chk1 by the purified ATR-ATRIP complex. Checkpoint defective RPA mutants fail to activate ATR kinase in this system, supporting the conclusion that this system is a faithful representation of the in vivo reaction. Interestingly, we find that an alternative form of RPA (aRPA), which does not support DNA replication, can substitute for the checkpoint function of RPA in vitro, thus revealing a potential role for aRPA in the activation of ATR kinase. We also find that TopBP1 is recruited to RPA-ssDNA in a manner dependent on ATRIP and that the N terminus of TopBP1 is required for efficient recruitment and activation of ATR kinase.

MIR-ATR sensor for process monitoring

International Nuclear Information System (INIS)

Geörg, Daniel; Schalk, Robert; Beuermann, Thomas; Methner, Frank-Jürgen

2015-01-01

A mid-infrared attenuated total reflectance (MIR-ATR) sensor has been developed for chemical reaction monitoring. The optical setup of the compact and low-priced sensor consists of an IR emitter as light source, a zinc selenide (ZnSe) ATR prism as boundary to the process, and four thermopile detectors, each equipped with an optical bandpass filter. The practical applicability was tested during esterification of ethanol and formic acid to ethyl formate and water as a model reaction with subsequent distillation. For reference analysis, a Fourier transform mid-infrared (FT-MIR) spectrometer with diamond ATR module was applied. On-line measurements using the MIR-ATR sensor and the FT-MIR spectrometer were performed in a bypass loop. The sensor was calibrated by multiple linear regression in order to link the measured absorbance in the four optical channels to the analyte concentrations. The analytical potential of the MIR-ATR sensor was demonstrated by simultaneous real-time monitoring of all four chemical substances involved in the esterification and distillation process. The temporal courses of the sensor signals are in accordance with the concentration values achieved by the commercial FT-MIR spectrometer. The standard error of prediction for ethanol, formic acid, ethyl formate, and water were 0.38 mol L   −  1 , 0.48 mol L   −  1 , 0.38 mol L   −  1 , and 1.12 mol L   −  1 , respectively. A procedure based on MIR spectra is presented to simulate the response characteristics of the sensor if the transmission ranges of the filters are varied. Using this tool analyte specific bandpass filters for a particular chemical reaction can be identified. By exchanging the optical filters, the sensor can be adapted to a wide range of processes in the chemical, pharmaceutical, and beverage industries. (paper)

Grayscale optical correlator for real-time onboard ATR

Science.gov (United States)

Chao, Tien-Hsin; Zhou, Hanying; Reyes, George F.

2001-03-01

Jet Propulsion Laboratory has been developing grayscale optical correlator (GOC) for a variety of automatic target recognition (ATR) applications. As reported in previous papers, a 128 X 128 camcorder-sized GOC has been demonstrated for real-time field ATR demos. In this paper, we will report the recent development of a prototype 512 X 512 GOC utilizing a new miniature ferroelectric liquid crystal spatial light modulator with a 7-micrometers pixel pitch. Experimental demonstration of ATR applications using this new GOC will be presented. The potential of developing a matchbox-sized GOC will also be discussed. A new application of synthesizing new complex-valued correlation filters using this real-axis 512 X 512 SLM will also be included.

Mislocalization of the MRN complex prevents ATR signaling during adenovirus infection

DEFF Research Database (Denmark)

Carson, Christian T; Orazio, Nicole I; Lee, Darwin V

2009-01-01

The protein kinases ataxia-telangiectasia mutated (ATM) and ATM-Rad3 related (ATR) are activated in response to DNA damage, genotoxic stress and virus infections. Here we show that during infection with wild-type adenovirus, ATR and its cofactors RPA32, ATRIP and TopBP1 accumulate at viral...... during virus infection, which is independent of Mre11 nuclease activity and recruitment of RPA/ATR/ATRIP/TopBP1. Unlike other damage scenarios, we found that ATM and ATR signaling are not dependent on each other during infection. We identify a region of the viral E4orf3 protein responsible...

An ATR architecture for algorithm development and testing

Science.gov (United States)

Breivik, Gøril M.; Løkken, Kristin H.; Brattli, Alvin; Palm, Hans C.; Haavardsholm, Trym

2013-05-01

A research platform with four cameras in the infrared and visible spectral domains is under development at the Norwegian Defence Research Establishment (FFI). The platform will be mounted on a high-speed jet aircraft and will primarily be used for image acquisition and for development and test of automatic target recognition (ATR) algorithms. The sensors on board produce large amounts of data, the algorithms can be computationally intensive and the data processing is complex. This puts great demands on the system architecture; it has to run in real-time and at the same time be suitable for algorithm development. In this paper we present an architecture for ATR systems that is designed to be exible, generic and efficient. The architecture is module based so that certain parts, e.g. specific ATR algorithms, can be exchanged without affecting the rest of the system. The modules are generic and can be used in various ATR system configurations. A software framework in C++ that handles large data ows in non-linear pipelines is used for implementation. The framework exploits several levels of parallelism and lets the hardware processing capacity be fully utilised. The ATR system is under development and has reached a first level that can be used for segmentation algorithm development and testing. The implemented system consists of several modules, and although their content is still limited, the segmentation module includes two different segmentation algorithms that can be easily exchanged. We demonstrate the system by applying the two segmentation algorithms to infrared images from sea trial recordings.

ATR, Radiation Transport Models in Atmosphere at Various Altitudes

International Nuclear Information System (INIS)

1981-01-01

1 - Description of problem or function: ATR is a user-oriented code for calculating quickly and simply radiation environment problems at all altitudes in the atmosphere. The code is based on parametric models of a comprehensive data base of air transport results which were generated using discrete ordinates transport techniques for infinite homogeneous air. The effects of air-ground interface and non-uniform air density are treated as perturbation corrections on homogeneous air results. ATR includes parametric models for neutrons and secondary gamma rays as a function of space, energy and source- target angle out to angles of 550 g/cm 2 of air. ATR contains parameterizations of infinite medium air transport of neutrons and secondary gamma rays and correction factors for the air-ground interface and high altitude exponential air. It responds to a series of user-oriented commands which specify the source, geometry and print options to output a variety of useful air transport information, including energy-angle dependent fluence, dose, current, and isodose ranges. 2 - Method of solution: The version 3 differs from earlier versions in that version 3 contains the parameterization of the new neutron and secondary gamma rays data base that was calculated using the latest DNA approved cross sections for air. Other improvements to the ATR code include: parameterization and inclusion into ATR of new air- over-ground correction factors, low energy x-rays calculations, new fission source, and new convenience options. 3 - Restrictions on the complexity of the problem: ATR takes approximately 36,000 decimal words of storage. This can be lessened by overlaying different parts of the code

Functional analyses of ATM, ATR and Fanconi anemia proteins in lung carcinoma

International Nuclear Information System (INIS)

Beumer, Jan H.; Fu, Katherine Y.; Anyang, Bean N.; Siegfried, Jill M.; Bakkenist, Christopher J.

2015-01-01

ATM and ATR are kinases implicated in a myriad of DNA-damage responses. ATM kinase inhibition radiosensitizes cells and selectively kills cells with Fanconi anemia (FA) gene mutations. ATR kinase inhibition sensitizes cells to agents that induce replication stress and selectively kills cells with ATM and TP53 mutations. ATM mutations and FANCF promoter-methylation are reported in lung carcinomas. We undertook functional analyses of ATM, ATR, Chk1 and FA proteins in lung cancer cell lines. We included Calu6 that is reported to be FANCL-deficient. In addition, the cancer genome atlas (TCGA) database was interrogated for alterations in: 1) ATM, MRE11A, RAD50 and NBN; 2) ATR, ATRIP and TOPBP1; and 3) 15 FA genes. No defects in ATM, ATR or Chk1 kinase activation, or FANCD2 monoubiquitination were identified in the lung cancer cell lines examined, including Calu6, and major alterations in these pathways were not identified in the TCGA database. Cell lines were radiosensitized by ATM kinase inhibitor KU60019, but no cell killing by ATM kinase inhibitor alone was observed. While no synergy between gemcitabine or carboplatin and ATR kinase inhibitor ETP-46464 was observed, synergy between gemcitabine and Chk1 kinase inhibitor UCN-01 was observed in 54 T, 201 T and H460, and synergy between carboplatin and Chk1 kinase inhibitor was identified in 201 T and 239 T. No interactions between ATM, ATR and FA activation were observed by either ATM or ATR kinase inhibition in the lung cancer cell lines. Analyses of ATM serine 1981 and Chk1 serine 345 phosphorylation, and FANCD2 monoubiquitination revealed that ATM and ATR kinase activation and FA pathway signaling are intact in the lung cancer cell lines examined. As such, these posttranslational modifications may have utility as biomarkers for the integrity of DNA damage signaling pathways in lung cancer. Different sensitization profiles between gemcitabine and carboplatin and ATR kinase inhibitor ETP-46464 and Chk1 kinase inhibitor

In-situ ATR-FTIR for characterization of thin biorelated polymer films

International Nuclear Information System (INIS)

Müller, M.; Torger, B.; Bittrich, E.; Kaul, E.; Ionov, L.; Uhlmann, P.; Stamm, M.

2014-01-01

We present and review in-situ-attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopic data from thin biorelated polymer films useful for the modification and functionalization of polymer and inorganic materials and discuss their applications related to life sciences. A special ATR mirror attachment operated by the single-beam-sample-reference (SBSR) concept and housing a homebuilt thermostatable flow cell was used, which allows for appropriate background compensation and signal to noise ratio. ATR-FTIR data on the reactive deposition of dopamine on inorganic model surfaces are shown. Information on the structure and deposition pathway for such bioinspired melanin-like films is provided. ATR-FTIR data on thermosensitive polymer brushes of poly(N-isopropylacrylamide) (PNIPAAM) is then presented. The thermotropic hydration and hydrogen bonding behavior of PNIPAAM brush films is described. Finally, ATR-FTIR data on biorelated polyelectrolyte multilayers (PEM) are given together with details on PEM growth and detection. Applications of these latter films for biopassivation/activation and local drug delivery are addressed

Mutation of serine 1333 in the ATR HEAT repeats creates a hyperactive kinase.

Directory of Open Access Journals (Sweden)

Jessica W Luzwick

Full Text Available Subcellular localization, protein interactions, and post-translational modifications regulate the DNA damage response kinases ATR, ATM, and DNA-PK. During an analysis of putative ATR phosphorylation sites, we found that a single mutation at S1333 creates a hyperactive kinase. In vitro and in cells, mutation of S1333 to alanine (S1333A-ATR causes elevated levels of kinase activity with and without the addition of the protein activator TOPBP1. S1333 mutations to glycine, arginine, or lysine also create a hyperactive kinase, while mutation to aspartic acid decreases ATR activity. S1333A-ATR maintains the G2 checkpoint and promotes completion of DNA replication after transient exposure to replication stress but the less active kinase, S1333D-ATR, has modest defects in both of these functions. While we find no evidence that S1333 is phosphorylated in cultured cells, our data indicate that small changes in the HEAT repeats can have large effects on kinase activity. These mutants may serve as useful tools for future studies of the ATR pathway.

ATR Prohibits Replication Catastrophe by Preventing Global Exhaustion of RPA

DEFF Research Database (Denmark)

Toledo Lazaro, Luis Ignacio; Altmeyer, Matthias; Rask, Maj-Britt

2013-01-01

origin firing generates an excess of single-stranded DNA that exhausts the nuclear pool of RPA. Partial reduction of RPA accelerated fork breakage, and forced elevation of RPA was sufficient to delay such "replication catastrophe" even in the absence of ATR activity. Conversely, unscheduled origin firing...... induced breakage of stalled forks even in cells with active ATR. Thus, ATR-mediated suppression of dormant origins shields active forks against irreversible breakage via preventing exhaustion of nuclear RPA. This study elucidates how replicating genomes avoid destabilizing DNA damage. Because cancer cells...

Development of leak detection system using high temperature-resistant microphones

International Nuclear Information System (INIS)

Morishita, Yoshitsugu; Mochizuki, Hiroyasu; Watanabe, Kenshiu; Nakamura, Takahisa; Nakazima, Yoshiaki; Yamauchi, Tatsuya

1995-01-01

This report describes the development and testing of a coolant leak detection system for an inlet feeder pipe of an advanced thermal reactor (ATR) using high temperature-resistant microphones. Such microphones must be resistant to both high temperatures and high radiation doses. Leakage sound characteristics, attenuation of the sound level in a heat insulating box for the inlet feeder pipes, and background noise were investigated using the experimental facility and the prototype ATR 'FUGEN'. The optimum frequency ranges for the microphone were then determined based on the observed leakage sound and background noise. The ability of the microphone to discriminate between leaks and other burst-type noises was also investigated by statistical analyses. Finally, it was confirmed that the present method could detect a leak within a couple of seconds. (author)

ATR National Scientific User Facility 2009 Annual Report

Energy Technology Data Exchange (ETDEWEB)

Todd R. Allen; Mitchell K. Meyer; Frances Marshall; Mary Catherine Thelen; Jeff Benson

2010-11-01

This report describes activities of the ATR NSUF from FY-2008 through FY-2009 and includes information on partner facilities, calls for proposals, users week and education programs. The report also contains project information on university research projects that were awarded by ATR NSUF in the fiscal years 2008 & 2009. This research is university-proposed researcher under a user facility agreement. All intellectual property from these experiments belongs to the university per the user agreement.

ATR Technical Specification Upgrade Program

International Nuclear Information System (INIS)

McCracken, R.T.; Durney, J.L.; Freund, G.A.

1990-01-01

The Advanced Test Reactor (ATR) is a 250 MW, uranium-aluminum fueled test reactor which began full power operation in 1969. The initial operation was controlled by an Operating Limits document based on the original Safety Analysis Report. Additional safety bases were later developed to support Technical Specifications which were approved and implemented in 1977. The Technical Specifications which were initially developed with content and format specified in ANSI/ANS--15.1, ''The Development of Technical Specifications for Research Reactors.'' The safety basis documentation and the Technical Specifications have been updated as required to maintain them current with the ATR facility configuration. All revisions have been made with a content, format and style consistent with the original. A major, two-phase program to upgrade the content, format and style is in progress. This paper describes the first phase of this program

Web-Enabled ATR/Fusion Development System

National Research Council Canada - National Science Library

Ruda, Harald

2001-01-01

.... We have designed a Web-Enabled ATR/Fusion Development System (WEADS) that will allow distributed development and execution of AIR and fusion algorithms using currently available infrastructures...

ATR National Scientific User Facility 2009 Annual Report

International Nuclear Information System (INIS)

Allen, Todd R.; Meyer, Mitchell K.; Marshall, Frances; Thelen, Mary Catherine; Benson, Jeff

2010-01-01

This report describes activities of the ATR NSUF from FY-2008 through FY-2009 and includes information on partner facilities, calls for proposals, users week and education programs. The report also contains project information on university research projects that were awarded by ATR NSUF in the fiscal years 2008 and 2009. This research is university-proposed researcher under a user facility agreement. All intellectual property from these experiments belongs to the university per the user agreement.

ATR kinase regulates its attenuation via PPM1D phosphatase ...

Indian Academy of Sciences (India)

In eukaryotes, in response to replication stress, DNA damage response kinase, ATR is activated, whose signalling abrogationleads to cell lethality due to aberrant fork remodelling and excessive origin firing. Here we report that inhibition ofATR kinase activity specifically during replication stress recovery results in persistent ...

Research on decommissioning of nuclear facilities 2. Study on optimum scenario using the AHP (Joint research)

International Nuclear Information System (INIS)

Shibahara, Yuji; Morishita, Yoshitsugu; Ishigami, Tsutomu; Yanagihara, Satoshi; Arita, Yuji

2013-01-01

To implement a decommissioning project reasonably, it is necessary and important to calculate project management data beforehand as well as to select an optimum dismantling scenario among various scenarios postulated. Little study on the subject of selecting an optimum scenario has been carried out, and it is one of the most important issues in terms of decision making. In FY 2009, Japan Atomic Energy Agency and University of Fukui launched the joint research of a decision making method which is important to determine a decommissioning plan. The purpose of this research is to formulate a methodology for selecting an optimum dismantling scenario among various scenarios postulated based on calculated results of project management data for FUGEN. Project management data for several dismantling scenarios postulated at FUGEN were evaluated based on actual dismantling work for feedwater heater at FUGEN, and an optimum scenario was discussed using the AHP, one of Multi-Criteria Decision Analysis Methods. This report describes the results of the joint research in FY 2010. (author)

Blueprint for domestic uranium enrichment

International Nuclear Information System (INIS)

1981-01-01

The AEC advisory committee on domestic production of uranium enrichment has studied for more than a year how to achieve the domestic enrichment of uranium by the construction and operation of a commercial enriching plant using centrifugal separation method, and the report was submitted to the Atomic Energy Commission on August 18, 1980. Japan has depended wholly on overseas services for her uranium enrichment needs, but the development of domestic enrichment has been carried on in parallel. The AEC decided to construct a uranium enrichment pilot plant using centrifuges, and it has been forwarded as a national project. The plant is operated by the Power Reactor and Nuclear Fuel Development Corp. since 1979. The capacity of the plant will be raised to approximately 75 ton SWU a year. The centrifuges already operated have provided the first delivery of fuel of about 1 ton for the ATR ''Fugen''. The demand-supply balance of uranium enrichment service, the significance of the domestic enrichment of uranium, the evaluation of uranium enrichment technology, the target for domestic enrichment plan, the measures to promote domestic uranium enrichment, and the promotion of the construction of a demonstration plant are reported. (Kako, I.)

Status report of the ATR SPING-3A and detailed calibration guideline

International Nuclear Information System (INIS)

Koeppen, L.D.; Rogers, J.W.

1985-12-01

The Radiation Measurements Laboratory (RML) onducted testing and an evaluation of the ATR SPING-3 after installation of the new ''A'' series software (firmware) and retrofit kit. The purpose of this report is to provide the ATR SPING-3A users with a document describing the present status of the unit, how the system was tested, a suggested calibration guideline, how the system should be operated for the ATR application, and why it should be used in this manner

Present status and future outlook of nuclear power generation in Japan

International Nuclear Information System (INIS)

Kunikazu Aisaka

1987-01-01

The structure of energy consumption in Japan is heavily dependent on imported oil, therefore Japan has been making its greatest effort in developing nuclear power among other alternatives of oil. The capacity factor of the nuclear power plants in Japan marked 76% in FY 1986, exceeding 70% level for the past several years. The share of nuclear power is expected to increase steadily in the future. Future scale of the nuclear power generation is projected as 62,000 MW in year 2000 and as 137,000 MW in 2030. Nuclear power is expected to produce 58% of the nation's total power generation in 2030. Under the present circumstances, Janpan is executing a nuclear energy policy based on the following guidelines: 1. Promoting the safety advancement program; 2. Improving LWR technologies; 3. Program on use of plutonium in thermal reactors; 4. Advanced thermal reactors (ATRs); 5. Promotion of FBR development; 6. Nuclear fuel cycle. (Liu)

Homeostatic regulation of meiotic DSB formation by ATM/ATR

International Nuclear Information System (INIS)

Cooper, Tim J.; Wardell, Kayleigh; Garcia, Valerie; Neale, Matthew J.

2014-01-01

Ataxia–telangiectasia mutated (ATM) and RAD3-related (ATR) are widely known as being central players in the mitotic DNA damage response (DDR), mounting responses to DNA double-strand breaks (DSBs) and single-stranded DNA (ssDNA) respectively. The DDR signalling cascade couples cell cycle control to damage-sensing and repair processes in order to prevent untimely cell cycle progression while damage still persists [1]. Both ATM/ATR are, however, also emerging as essential factors in the process of meiosis; a specialised cell cycle programme responsible for the formation of haploid gametes via two sequential nuclear divisions. Central to achieving accurate meiotic chromosome segregation is the introduction of numerous DSBs spread across the genome by the evolutionarily conserved enzyme, Spo11. This review seeks to explore and address how cells utilise ATM/ATR pathways to regulate Spo11-DSB formation, establish DSB homeostasis and ensure meiosis is completed unperturbed

Homeostatic regulation of meiotic DSB formation by ATM/ATR

Energy Technology Data Exchange (ETDEWEB)

Cooper, Tim J.; Wardell, Kayleigh; Garcia, Valerie; Neale, Matthew J., E-mail: m.neale@sussex.ac.uk

2014-11-15

Ataxia–telangiectasia mutated (ATM) and RAD3-related (ATR) are widely known as being central players in the mitotic DNA damage response (DDR), mounting responses to DNA double-strand breaks (DSBs) and single-stranded DNA (ssDNA) respectively. The DDR signalling cascade couples cell cycle control to damage-sensing and repair processes in order to prevent untimely cell cycle progression while damage still persists [1]. Both ATM/ATR are, however, also emerging as essential factors in the process of meiosis; a specialised cell cycle programme responsible for the formation of haploid gametes via two sequential nuclear divisions. Central to achieving accurate meiotic chromosome segregation is the introduction of numerous DSBs spread across the genome by the evolutionarily conserved enzyme, Spo11. This review seeks to explore and address how cells utilise ATM/ATR pathways to regulate Spo11-DSB formation, establish DSB homeostasis and ensure meiosis is completed unperturbed.

ARF and ATM/ATR cooperate in p53-mediated apoptosis upon oncogenic stress

International Nuclear Information System (INIS)

Pauklin, Siim; Kristjuhan, Arnold; Maimets, Toivo; Jaks, Viljar

2005-01-01

Induction of apoptosis is pivotal for eliminating cells with damaged DNA or deregulated proliferation. We show that tumor suppressor ARF and ATM/ATR kinase pathways cooperate in the induction of apoptosis in response to elevated expression of c-myc, β-catenin or human papilloma virus E7 oncogenes. Overexpression of oncogenes leads to the formation of phosphorylated H2AX foci, induction of Rad51 protein levels and ATM/ATR-dependent phosphorylation of p53. Inhibition of ATM/ATR kinases abolishes both induction of Rad51 and phosphorylation of p53, and remarkably reduces the level of apoptosis induced by co-expression of oncogenes and ARF. However, the induction of apoptosis is downregulated in p53-/- cells and does not depend on activities of ATM/ATR kinases, indicating that efficient induction of apoptosis by oncogene activation depends on coordinated action of ARF and ATM/ATR pathways in the regulation of p53

Protein phosphatase 5 is necessary for ATR-mediated DNA repair

International Nuclear Information System (INIS)

Kang, Yoonsung; Cheong, Hyang-Min; Lee, Jung-Hee; Song, Peter I.; Lee, Kwang-Ho; Kim, Sang-Yong; Jun, Jae Yeoul; You, Ho Jin

2011-01-01

Research highlights: → Serine/threonine protein phosphatase 5 (PP5) has been shown to participate in ataxia telangiectasia-mutated (ATM)- and ATR (ATM- and Rad3-related)-mediated checkpoint pathways, which plays an important role in the DNA damage response and maintenance of genomic stability. → However, it is not clear exactly how PP5 participates in this process. → Our results indicate that PP5 is more closely related with ATR-mediated pathway than ATM-mediated pathway in DNA damage repair. -- Abstract: Several recent studies have shown that protein phosphatase 5 (PP5) participates in cell cycle arrest after DNA damage, but its roles in DNA repair have not yet been fully characterized. We investigated the roles of PP5 in the repair of ultraviolet (UV)- and neocarzinostatin (NCS)-induced DNA damage. The results of comet assays revealed different repair patterns in UV- and NCS-exposed U2OS-PS cells. PP5 is only essential for Rad3-related (ATR)-mediated DNA repair. Furthermore, the phosphorylation of 53BP1 and BRCA1, important mediators of DNA damage repair, and substrates of ATR and ATM decreased in U2OS-PS cells exposed to UV radiation. In contrast, the cell cycle arrest proteins p53, CHK1, and CHK2 were normally phosphorylated in U2OS and U2OS-PS cells exposed to UV radiation or treated with NCS. In view of these results, we suggest that PP5 plays a crucial role in ATR-mediated repair of UV-induced DNA damage.

ATM and ATR play complementary roles in the behavior of excitatory and inhibitory vesicle populations.

Science.gov (United States)

Cheng, Aifang; Zhao, Teng; Tse, Kai-Hei; Chow, Hei-Man; Cui, Yong; Jiang, Liwen; Du, Shengwang; Loy, Michael M T; Herrup, Karl

2018-01-09

ATM (ataxia-telangiectasia mutated) and ATR (ATM and Rad3-related) are large PI3 kinases whose human mutations result in complex syndromes that include a compromised DNA damage response (DDR) and prominent nervous system phenotypes. Both proteins are nuclear-localized in keeping with their DDR functions, yet both are also found in cytoplasm, including on neuronal synaptic vesicles. In ATM- or ATR-deficient neurons, spontaneous vesicle release is reduced, but a drop in ATM or ATR level also slows FM4-64 dye uptake. In keeping with this, both proteins bind to AP-2 complex components as well as to clathrin, suggesting roles in endocytosis and vesicle recycling. The two proteins play complementary roles in the DDR; ATM is engaged in the repair of double-strand breaks, while ATR deals mainly with single-strand damage. Unexpectedly, this complementarity extends to these proteins' synaptic function as well. Superresolution microscopy and coimmunoprecipitation reveal that ATM associates exclusively with excitatory (VGLUT1 + ) vesicles, while ATR associates only with inhibitory (VGAT + ) vesicles. The levels of ATM and ATR respond to each other; when ATM is deficient, ATR levels rise, and vice versa. Finally, blocking NMDA, but not GABA, receptors causes ATM levels to rise while ATR levels respond to GABA, but not NMDA, receptor blockade. Taken together, our data suggest that ATM and ATR are part of the cellular "infrastructure" that maintains the excitatory/inhibitory balance of the nervous system. This idea has important implications for the human diseases resulting from their genetic deficiency.

Initial characterization of the ATR [Advanced Test Reactor] Large Gamma Facility

International Nuclear Information System (INIS)

Schnitzler, B.G.; Rogers, J.W.

1986-05-01

Radiation fields in the ATR Large Gamma Facility test volume are characterized. The preliminary characterization efforts described in this report include total dose rate measurements in the facility, development of a simple methodology for calculating radiation fields from the ATR fuel element power histories, and a comparison of the measured and calculated values

On civil and architectural engineering works of the new prototype converter FUGEN

International Nuclear Information System (INIS)

Kiri, Akira; Yamagata, Takayasu.

1976-01-01

The relatively featured engineering work of FUGEN is reviewed, and the outline of ventilation system is illustrated. Civil engineering work commenced in 1970 and almost ended after 5 years. Test grout led to the practicability of injecting about 40 kg of milky cement per 1 meter of a drilled well. Ten meter long wells around the reactor building base and 6 meter long wells inside the base were staggeredly arranged at 3 meter intervals. They were drilled with a 46 mm boring machine. The drifter drilling method could not be employed because of hard rock joint. The place and method of disposing dredged soil, the securing of time and place for setting marine pipes, and the prevention of turbid current inflow during dredging had to be solved for cooling water intake. The turbid current inflow was avoided with a large oil fence and chemical solution. The reinforcing steel for concrete was prefabricated. Dry pack method was employed in the works under the containment vessel. The ventilation system covers whole buildings except the air recirculating system for the containment vessel. For the purpose of removing radioactively contaminated dusts, cooling was performed, as a rule, by once-through ventilation system with 100% outdoor air. The air flows in the direction from clean zone to high contamination zone through low contamination zone. The iodine-removing efficiency of a filter system is 90%. (Iwakiri, K.)

The application of AI-based refueling control system to the Fugen NPP

International Nuclear Information System (INIS)

Maeda, Koichi; Nakamura, Shinji; Kawasaki, Noboru; Nanko, Takashi; Nishimura, Hiroshi; Tanaka, Koichi

1996-01-01

In a refueling operation of the Advanced Thermal Reactor Fugen, it is essential to precisely handle fuel assemblies to guarantee quality and from the standpoint of safety guard. In order to plan and execute efficient operations, knowledge of experienced operators based on experiences is required. Therefore, an automatic control system based on the Artificial Intelligence (AI) technology has been developed, and has been put into operation in actual refueling operations. This system prepares an optimum refueling plan, controls the fuel handling facilities by that plan, displays and prints out the history of transfer and storage of items by automatically sampled data on a work station, a network to express fuel handling facilities and operational rules acquired through operating experiences. The development of this system has made it possible to optimize refueling schedule, save control labor of items and improve reliability of refueling. Firstly the development started the off-line system that prepares optimum refueling plan and gives to the operator step by step instructions for operation, secondly developed the on-line system that automatically controls facilities by that plan. The development of this system started in 1989, the off-line system has been put into practical operation in the 17th refueling in 1991, the 22nd (12th annual inspection) refueling in 1995 has been done by using the on-line system automatically

PUREX new substation ATR

International Nuclear Information System (INIS)

Nelson, D.E.

1997-01-01

This document is the acceptance test report (ATR) for the New PUREX Main and Minisubstations. It covers the factory and vendor acceptance and commissioning test reports. Reports are presented for the Main 5 kV substation building, the building fire system, switchgear, and vacuum breaker; the minisubstation control building and switch gear; commissioning test; electrical system and loads inspection; electrical utilities transformer and cable; and relay setting changes based on operational experience

ATR and transmission analysis of pigments by means of far infrared spectroscopy.

Science.gov (United States)

Kendix, Elsebeth L; Prati, Silvia; Joseph, Edith; Sciutto, Giorgia; Mazzeo, Rocco

2009-06-01

In the field of FTIR spectroscopy, the far infrared (FIR) spectral region has been so far less investigated than the mid-infrared (MIR), even though it presents great advantages in the characterization of those inorganic compounds, which are inactive in the MIR, such as some art pigments, corrosion products, etc. Furthermore, FIR spectroscopy is complementary to Raman spectroscopy if the fluorescence effects caused by the latter analytical technique are considered. In this paper, ATR in the FIR region is proposed as an alternative method to transmission for the analyses of pigments. This methodology was selected in order to reduce the sample amount needed for analysis, which is a must when examining cultural heritage materials. A selection of pigments have been analyzed in both ATR and transmission mode, and the resulting spectra were compared with each other. To better perform this comparison, an evaluation of the possible effect induced by the thermal treatment needed for the preparation of the polyethylene pellets on the transmission spectra of the samples has been carried out. Therefore, pigments have been analyzed in ATR mode before and after heating them at the same temperature employed for the polyethylene pellet preparation. The results showed that while the heating treatment causes only small changes in the intensity of some bands, the ATR spectra were characterized by differences in both intensity and band shifts towards lower frequencies if compared with those recorded in transmission mode. All pigments' transmission and ATR spectra are presented and discussed, and the ATR method was validated on a real case study.

Development of a leak detection system using high temperature-resistant microphones

International Nuclear Information System (INIS)

Morishita, Yoshitsugu; Mochizuki, Hiroyasu; Watanabe, Kenshiu; Nakamura, Takahisa; Nakajima, Yoshiaki; Yamauchi, Tatsuya

1991-01-01

This report describes the development of a detection system of coolant leak from an inlet feeder pipe of an Advanced Thermal Reactor (ATR) with high temperature-resistant microphones. A microphone having resistance to both high temperature and high radiation dose has been developed at first. The characteristics with regard to leakage sound, attenuation of sound level in a heat insulating box for the inlet feeder pipes and background noise were clarified by laboratory experiments and measurements in the prototype ATR 'Fugen'. On the basis of these experimental findings, appropriate frequency ranges were surveyed to detect the leakage sound with a high S/N ratio under the background noise. Reliability of the system to a malfunction caused by burst-type noises observed in the plant was also investigated by statistical analyses. Finally, it was confirmed that the present method could detect a leak within a couple of seconds. (author)

Research on decommissioning of nuclear facilities (Joint research)

International Nuclear Information System (INIS)

Shibahara, Yuji; Morishita, Yoshitsugu; Ishigami, Tsutomu; Yanagihara, Satoshi; Arita, Yuji

2011-07-01

To implement a decommissioning project reasonably, it is necessary and important to beforehand evaluate project management data as well as to select an optimum dismantling scenario among various scenarios postulated. Little study on the subject of selecting an optimum scenario has been carried out, and it is one of the most important subjects in terms of decision making. In FY 2009, Japan Atomic Energy Agency and University of Fukui launched the joint research of a decision making method which is important to determine a decommissioning plan. The purpose of the research is to construct a methodology for selecting an optimum dismantling scenario among various scenarios postulated based on calculated results of project management data for FUGEN. Project management data for several dismantling scenarios postulated at FUGEN were evaluated based on actual dismantling work for feed water heater at FUGEN, and an optimum scenario was discussed using the SMART, one of Multi-Criteria Decision Analysis Method. This report describes the results of the joint research in FY 2009. (author)

DNA-PKcs, ATM, and ATR Interplay Maintains Genome Integrity during Neurogenesis.

Science.gov (United States)

Enriquez-Rios, Vanessa; Dumitrache, Lavinia C; Downing, Susanna M; Li, Yang; Brown, Eric J; Russell, Helen R; McKinnon, Peter J

2017-01-25

The DNA damage response (DDR) orchestrates a network of cellular processes that integrates cell-cycle control and DNA repair or apoptosis, which serves to maintain genome stability. DNA-PKcs (the catalytic subunit of the DNA-dependent kinase, encoded by PRKDC), ATM (ataxia telangiectasia, mutated), and ATR (ATM and Rad3-related) are related PI3K-like protein kinases and central regulators of the DDR. Defects in these kinases have been linked to neurodegenerative or neurodevelopmental syndromes. In all cases, the key neuroprotective function of these kinases is uncertain. It also remains unclear how interactions between the three DNA damage-responsive kinases coordinate genome stability, particularly in a physiological context. Here, we used a genetic approach to identify the neural function of DNA-PKcs and the interplay between ATM and ATR during neurogenesis. We found that DNA-PKcs loss in the mouse sensitized neuronal progenitors to apoptosis after ionizing radiation because of excessive DNA damage. DNA-PKcs was also required to prevent endogenous DNA damage accumulation throughout the adult brain. In contrast, ATR coordinated the DDR during neurogenesis to direct apoptosis in cycling neural progenitors, whereas ATM regulated apoptosis in both proliferative and noncycling cells. We also found that ATR controls a DNA damage-induced G 2 /M checkpoint in cortical progenitors, independent of ATM and DNA-PKcs. These nonoverlapping roles were further confirmed via sustained murine embryonic or cortical development after all three kinases were simultaneously inactivated. Thus, our results illustrate how DNA-PKcs, ATM, and ATR have unique and essential roles during the DDR, collectively ensuring comprehensive genome maintenance in the nervous system. The DNA damage response (DDR) is essential for prevention of a broad spectrum of different human neurologic diseases. However, a detailed understanding of the DDR at a physiological level is lacking. In contrast to many in

Advanced Test Reactor (ATR) Facility 10CFR830 Safety Basis Related to Facility Experiments

International Nuclear Information System (INIS)

Tomberlin, T.A.

2002-01-01

The Idaho National Engineering and Environmental Laboratory (INEEL) Advanced Test Reactor (ATR), a DOE Category A reactor, was designed to provide an irradiation test environment for conducting a variety of experiments. The ATR Safety Analysis Report, determined by DOE to meet the requirements of 10 CFR 830, Subpart B, provides versatility in types of experiments that may be conducted. This paper addresses two general types of experiments in the ATR facility and how safety analyses for experiments are related to the ATR safety basis. One type of experiment is more routine and generally represents greater risks; therefore this type of experiment is addressed with more detail in the safety basis. This allows individual safety analyses for these experiments to be more routine and repetitive. The second type of experiment is less defined and is permitted under more general controls. Therefore, individual safety analyses for the second type of experiment tend to be more unique from experiment to experiment. Experiments are also discussed relative to ''major modifications'' and DOE-STD-1027-92. Application of the USQ process to ATR experiments is also discussed

L'Atrésie colique:À propos de deux cas

African Journals Online (AJOL)

... dans un tableau d'occlusion néonatale haute. L'atrésie colique était de découverte fortuite en per-opératoire, associée à une atrésie grêlique. Le patient a bénéficié d'une double stomie, mais les suites opératoires ont été marquées par le décès dans un tableau de choc septique. A la lumière des ces deux observations ...

A Shld1-controlled POT1a provides support for repression of ATR signaling at telomeres through RPA exclusion.

Science.gov (United States)

Gong, Yi; de Lange, Titia

2010-11-12

We previously proposed that POT1 prevents ATR signaling at telomeres by excluding RPA from the single-stranded TTAGGG repeats. Here, we use a Shld1-stabilized degron-POT1a fusion (DD-POT1a) to study the telomeric ATR kinase response. In the absence of Shld1, DD-POT1a degradation resulted in rapid and reversible activation of the ATR pathway in G1 and S/G2. ATR signaling was abrogated by shRNAs to ATR and TopBP1, but shRNAs to the ATM kinase or DNA-PKcs did not affect the telomere damage response. Importantly, ATR signaling in G1 and S/G2 was reduced by shRNAs to RPA. In S/G2, RPA was readily detectable at dysfunctional telomeres, and both POT1a and POT1b were required to exclude RPA and prevent ATR activation. In G1, the accumulation of RPA at dysfunctional telomeres was strikingly less, and POT1a was sufficient to repress ATR signaling. These results support an RPA exclusion model for the repression of ATR signaling at telomeres. Copyright © 2010 Elsevier Inc. All rights reserved.

Generation and assessment of turntable SAR data for the support of ATR development

Science.gov (United States)

Cohen, Marvin N.; Showman, Gregory A.; Sangston, K. James; Sylvester, Vincent B.; Gostin, Lamar; Scheer, C. Ruby

1998-10-01

Inverse synthetic aperture radar (ISAR) imaging on a turntable-tower test range permits convenient generation of high resolution two-dimensional images of radar targets under controlled conditions for testing SAR image processing and for supporting automatic target recognition (ATR) algorithm development. However, turntable ISAR images are often obtained under near-field geometries and hence may suffer geometric distortions not present in airborne SAR images. In this paper, turntable data collected at Georgia Tech's Electromagnetic Test Facility are used to begin to assess the utility of two- dimensional ISAR imaging algorithms in forming images to support ATR development. The imaging algorithms considered include a simple 2D discrete Fourier transform (DFT), a 2-D DFT with geometric correction based on image domain resampling, and a computationally-intensive geometric matched filter solution. Images formed with the various algorithms are used to develop ATR templates, which are then compared with an eye toward utilization in an ATR algorithm.

Characterization and sintering of niobium-ATR alumina

International Nuclear Information System (INIS)

Sibuya, N.H.; Iwasaki, H.; Suzuki, C.K.; Pinatti, D.G.

1987-01-01

In the niobium aluminothermy a slag is produced, composed mostly of alumina and other compounds such as niobium oxide and silica. The phase composition of this ATR alumina was characterized by X-ray powder diffractometry, and afterwards this alumina was subjected to leaching processes. It was noticed that the original content of 70% α-alumina in slag rose to 95% after the calcination. ATR alumina (leached and calcined, and without any treatment) was used to make pressed bodies which were fired in air at 1200 to 1400 0 C for 1 to 10,5 hours; and in vacuum at 1550 to 1800$0C for 2 hours. Characterization was done by density measurements, X-ray diffractometry and ultrasonic analysis. Ultrasonic analysis of some vacuum fired bodies showed londitudinal velocities close to the value found in literature. Correlation of several techniques measurements disclosed the niobium oxide interference in sintering. (Author) [pt

Efficacy of ATR inhibitors as single agents in Ewing sarcoma

DEFF Research Database (Denmark)

Nieto-Soler, Maria; Morgado-Palacin, Isabel; Lafarga, Vanesa

2016-01-01

Ewing sarcomas (ES) are pediatric bone tumors that arise from a driver translocation, most frequently EWS/FLI1. Current ES treatment involves DNA damaging agents, yet the basis for the sensitivity to these therapies remains unknown. Oncogene-induced replication stress (RS) is a known source...... efficacy in ES xenografts as single agents. Expression of EWS/FLI1 or EWS/ERG oncogenic translocations sensitizes non-ES cells to ATR inhibitors. Our data shed light onto the sensitivity of ES to genotoxic agents, and identify ATR inhibitors as a potential therapy for Ewing Sarcomas....

Roles of ATR1 paralogs YMR279c and YOR378w in boron stress tolerance

International Nuclear Information System (INIS)

Bozdag, Gonensin Ozan; Uluisik, Irem; Gulculer, Gulce Sila; Karakaya, Huseyin C.; Koc, Ahmet

2011-01-01

Highlights: → ATR1 paralog YMR279c plays role in boron detoxification. → YMR279c overexpression lowers cytoplasmic boron levels. → ATR1 paralog YOR378w has no roles in boron stress response. -- Abstract: Boron is a necessary nutrient for plants and animals, however excess of it causes toxicity. Previously, Atr1 and Arabidopsis Bor1 homolog were identified as the boron efflux pump in yeast, which lower the cytosolic boron concentration and help cells to survive in the presence of toxic amount of boron. In this study, we analyzed ATR1 paralogs, YMR279c and YOR378w, to understand whether they participate in boron stress tolerance in yeast. Even though these genes share homology with ATR1, neither their deletion rendered cells boron sensitive nor their expression was significantly upregulated by boron treatment. However, expression of YMR279, but not YOR378w, from the constitutive GAPDH promoter on a high copy plasmid provided remarkable boron resistance by decreasing intracellular boron levels. Thus our results suggest the presence of a third boron exporter, YMR279c, which functions similar to ATR1 and provides boron resistance in yeast.

Pressure-modulation dynamic attenuated-total-reflectance (ATR) FT-IR spectroscopy

Science.gov (United States)

Marcott, C.; Story, G. M.; Noda, I.; Bibby, A.; Manning, C. J.

1998-06-01

A single-reflectance attenuated-total-reflectance (ATR) accessory with a diamond internal-reflection element was modified by the addition of a piezoelectric transducer. Initial dynamic pressure-modulation experiments have been performed in the sample compartment of a step-scanning FT-IR spectrometer. A sinusoidal pressure modulation applied to samples of isotactic polypropylene and linear low density polyethylene resulted in dynamic responses which appear to be similar to those observed in previous dynamic 2D IR experiments. Preliminary pressure-modulation dynamic ATR results are also reported for a styrene-butadiene-styrene triblock copolymer. The new method has the advantages that a much wider variety of sample types and geometries can be studied and less sample preparation is required. Dynamic 2D IR experiments carried out by ATR no longer require thin films of large area and sufficient strength to withstand the dynamic strain applied by a rheometer. The ability to obtain dynamic IR spectroscopic information from a wider variety of sample types and thicknesses would greatly expand the amount of useful information that could be extracted from normally complicated, highly overlapped IR spectra.

SAR image dataset of military ground targets with multiple poses for ATR

Science.gov (United States)

Belloni, Carole; Balleri, Alessio; Aouf, Nabil; Merlet, Thomas; Le Caillec, Jean-Marc

2017-10-01

Automatic Target Recognition (ATR) is the task of automatically detecting and classifying targets. Recognition using Synthetic Aperture Radar (SAR) images is interesting because SAR images can be acquired at night and under any weather conditions, whereas optical sensors operating in the visible band do not have this capability. Existing SAR ATR algorithms have mostly been evaluated using the MSTAR dataset.1 The problem with the MSTAR is that some of the proposed ATR methods have shown good classification performance even when targets were hidden,2 suggesting the presence of a bias in the dataset. Evaluations of SAR ATR techniques are currently challenging due to the lack of publicly available data in the SAR domain. In this paper, we present a high resolution SAR dataset consisting of images of a set of ground military target models taken at various aspect angles, The dataset can be used for a fair evaluation and comparison of SAR ATR algorithms. We applied the Inverse Synthetic Aperture Radar (ISAR) technique to echoes from targets rotating on a turntable and illuminated with a stepped frequency waveform. The targets in the database consist of four variants of two 1.7m-long models of T-64 and T-72 tanks. The gun, the turret position and the depression angle are varied to form 26 different sequences of images. The emitted signal spanned the frequency range from 13 GHz to 18 GHz to achieve a bandwidth of 5 GHz sampled with 4001 frequency points. The resolution obtained with respect to the size of the model targets is comparable to typical values obtained using SAR airborne systems. Single polarized images (Horizontal-Horizontal) are generated using the backprojection algorithm.3 A total of 1480 images are produced using a 20° integration angle. The images in the dataset are organized in a suggested training and testing set to facilitate a standard evaluation of SAR ATR algorithms.

RPA70 depletion induces hSSB1/2-INTS3 complex to initiate ATR signaling

OpenAIRE

Kar, Ananya; Kaur, Manpreet; Ghosh, Tanushree; Khan, Md. Muntaz; Sharma, Aparna; Shekhar, Ritu; Varshney, Akhil; Saxena, Sandeep

2015-01-01

The primary eukaryotic single-stranded DNA-binding protein, Replication protein A (RPA), binds to single-stranded DNA at the sites of DNA damage and recruits the apical checkpoint kinase, ATR via its partner protein, ATRIP. It has been demonstrated that absence of RPA incapacitates the ATR-mediated checkpoint response. We report that in the absence of RPA, human single-stranded DNA-binding protein 1 (hSSB1) and its partner protein INTS3 form sub-nuclear foci, associate with the ATR-ATRIP comp...

VRdose: an exposure dose evaluation system based on virtual reality technology - current status and future possibilities

International Nuclear Information System (INIS)

Iguchi, Yukihiro; Louka, Michael; Johnsen, Terje

2004-01-01

The Fugen Nuclear Power Station (NPS) was shut down permanently in March 2003, and preparatory activities are underway to decommission the Fugen NPS. It is necessary to accomplish the decommissioning economically and rationally by optimizing the workload, exposure dose and waste mass. This is important at the planning stage of the decommissioning. Virtual reality (VR) technology may prove beneficial to this process with regard to minimizing the workers' radiation exposure as well as contributing towards achieving efficient use of manpower. It could also be a valuable tool in the actual dismantling phase. In addition to this, VR provides an effective medium in presentations for public acceptance as well as for communication with relevant engineers. The VRdose project conducted by Japan Nuclear Cycle Development Institute (JNC) and Halden Virtual Reality Centre is doing research and development of VR technology for use in the decommissioning process at the Fugen NPS. This is technically an extensive project, touching on many of the present challenges in the VR area such as visual simulation and animation, interaction with objects in a virtual environment and scenario generation and optimisation. This paper describes the present status and future of the system. (Author)

Small-molecule inhibitors of Ataxia Telangiectasia and Rad3 related kinase (ATR) sensitize lymphoma cells to UVA radiation

DEFF Research Database (Denmark)

Biskup, Edyta; Naym, David Gram; Gniadecki, Robert

2016-01-01

inhibited by small molecule antagonists VE-821, VE-822 or Chir-124, or by small interfering RNAs (siRNAs). Cell cycle and viability were assessed by flow cytometry. RESULTS: Small molecule inhibitors of ATR and Chk1 potently sensitized all cell lines to PUVA and, importantly, also to UVA, which by itself...... did not cause apoptotic response. VE-821/2 blocked ATR pathway activation and released the cells from the G2/M block caused by UVA and PUVA, but did not affect apoptosis caused by other chemotherapeutics (etoposide, gemcitabine, doxorubicine) or by hydrogen peroxide. Knockdown of ATR and Chk1 with si......RNA also blocked the ATR pathway and released the cells from G2/M block but did not sensitize the cells to UVA as observed with the small molecule inhibitors. The latter suggested that the synergism between VE-821/2 or Chir-124 and UVA was not solely caused by specific blocking of ATR kinase but also ATR...

ATM/ATR-mediated phosphorylation of PALB2 promotes RAD51 function

DEFF Research Database (Denmark)

Ahlskog, Johanna K; Larsen, Brian D; Achanta, Kavya

2016-01-01

DNA damage activates the ATM and ATR kinases that coordinate checkpoint and DNA repair pathways. An essential step in homology-directed repair (HDR) of DNA breaks is the formation of RAD51 nucleofilaments mediated by PALB2-BRCA2; however, roles of ATM and ATR in this critical step of HDR are poor...... function, as the PALB2-dependent checkpoint response is normal in cells expressing the phospho-deficient PALB2 mutant. Collectively, our findings highlight a critical importance of PALB2 phosphorylation as a novel regulatory step in genome maintenance after genotoxic stress....

AGR-3/4 Final Data Qualification Report for ATR Cycles 151A through 155B-1

Energy Technology Data Exchange (ETDEWEB)

Pham, Binh T. [Idaho National Lab. (INL), Idaho Falls, ID (United States)

2015-03-01

This report provides the qualification status of experimental data for the entire Advanced Gas Reactor 3/4 (AGR 3/4) fuel irradiation. AGR-3/4 is the third in a series of planned irradiation experiments conducted in the Advanced Test Reactor (ATR) at Idaho National Laboratory (INL) for the AGR Fuel Development and Qualification Program, which supports development of the advanced reactor technology under the INL ART Technology Development Office (TDO). The main objective of AGR-3/4 irradiation is to provide a known source of fission products for subsequent transport through compact matrix and structural graphite materials due to the presence of designed-to-fail fuel particles. Full power irradiation of the AGR 3/4 test began on December 14, 2011 (ATR Cycle 151A), and was completed on April 12, 2014 (end of ATR Cycle 155B) after 369.1 effective full power days of irradiation. The AGR-3/4 test was in the reactor core for eight of the ten ATR cycles between 151A and 155B. During the unplanned outage cycle, 153A, the experiment was removed from the ATR northeast flux trap (NEFT) location and stored in the ATR canal. This was to prevent overheating of fuel compacts due to higher than normal ATR power during the subsequent Powered Axial Locator Mechanism cycle, 153B. The AGR 3/4 test was inserted back into the ATR NEFT location during the outage of ATR Cycle 154A on April 26, 2013. Therefore, the AGR-3/4 irradiation data received during these 2 cycles (153A and 153B) are irrelevant and their qualification status isnot included in this report. Additionally, during ATR Cycle 152A the ATR core ran at low power for a short enough duration that the irradiation data are not used for physics and thermal calculations. However, the qualification status of irradiation data for this cycle is still covered in this report. As a result, this report includes data from 8 ATR Cycles: 151A, 151B, 152A, 152B, 154A, 154B, 155A, and 155B, as recorded in the Nuclear Data Management and

Human papillomavirus detection using PCR and ATR-FTIR for cervical cancer screening

Science.gov (United States)

Rymsza, Taciana; Ribeiro, Eliane Aline; de Carvalho, Luis Felipe das Chagas e. Silva; Bhattacharjee, Tanmoy; de Azevedo Canevari, Renata

2018-05-01

The human papillomavirus (HPV) genital infection is considered one of the most common sexually transmitted diseases worldwide, and has been associated with cervical cancer. The objective of this study was to investigate the efficacy of the diagnostic methods: polymerase chain reaction (PCR) and Fourier transform infrared (FTIR) equipped with an ATR (Attenuated Total Reflectance) unit (Pike Tech) spectroscopy, to diagnose HPV infection in women undergoing gynecological examination. Seventeen patients (41.46%) of the 41 patients analyzed were diagnosed with exophytic/condyloma acuminate lesions by clinical analysis, 29 patients (70.7%) (G1 group) of the 41 patients, showed positive result for HPV cell injury by oncotic colpocitology and 12 patients (29.3%) (G2 group), presented negative result for cellular lesion and absence of clinical HPV lesion. Four samples were obtained per patient, which were submitted oncotic colpocitology analysis (Papanicolau staining, two samples), PCR (one sample) and ATR-FTIR analysis (one sample). L1 gene was amplified by PCR technique with specific GP5+/GP6+ and MY09/MY11 primers. PCR results were uniformly positive for presence of HPV in all analyzed samples. Multivariate analysis of ATR-FTIR spectra suggests no significant biochemical changes between groups and no clustering formed, concurring with results of PCR. This study suggests that PCR and ATR-FTIR are highly sensitive technique for HPV detection.

RPA-Binding Protein ETAA1 Is an ATR Activator Involved in DNA Replication Stress Response.

Science.gov (United States)

Lee, Yuan-Cho; Zhou, Qing; Chen, Junjie; Yuan, Jingsong

2016-12-19

ETAA1 (Ewing tumor-associated antigen 1), also known as ETAA16, was identified as a tumor-specific antigen in the Ewing family of tumors. However, the biological function of this protein remains unknown. Here, we report the identification of ETAA1 as a DNA replication stress response protein. ETAA1 specifically interacts with RPA (Replication protein A) via two conserved RPA-binding domains and is therefore recruited to stalled replication forks. Interestingly, further analysis of ETAA1 function revealed that ETAA1 participates in the activation of ATR signaling pathway via a conserved ATR-activating domain (AAD) located near its N terminus. Importantly, we demonstrate that both RPA binding and ATR activation are required for ETAA1 function at stalled replication forks to maintain genome stability. Therefore, our data suggest that ETAA1 is a new ATR activator involved in replication checkpoint control. Copyright © 2016 Elsevier Ltd. All rights reserved.

Analysis of the ATR fuel element swaging process

International Nuclear Information System (INIS)

Richins, W.D.; Miller, G.K.

1995-12-01

This report documents a detailed evaluation of the swaging process used to connect fuel plates to side plates in Advanced Test Reactor (ATR) fuel elements. The swaging is a mechanical process that begins with fitting a fuel plate into grooves in the side plates. Once a fuel plate is positioned, a lip on each of two side plate grooves is pressed into the fuel plate using swaging wheels to form the joints. Each connection must have a specified strength (measured in terms, of a pullout force capacity) to assure that these joints do not fail during reactor operation. The purpose of this study is to analyze the swaging process and associated procedural controls, and to provide recommendations to assure that the manufacturing process produces swaged connections that meet the minimum strength requirement. The current fuel element manufacturer, Babcock and Wilcox (B ampersand W) of Lynchburg, Virginia, follows established procedures that include quality inspections and process controls in swaging these connections. The procedures have been approved by Lockheed Martin Idaho Technologies and are designed to assure repeatability of the process and structural integrity of each joint. Prior to July 1994, ATR fuel elements were placed in the Hydraulic Test Facility (HTF) at the Idaho National Engineering Laboratory (AGNAIL), Test Reactor Area (TRA) for application of Boehmite (an aluminum oxide) film and for checking structural integrity before placement of the elements into the ATR. The results presented in this report demonstrate that the pullout strength of the swaged connections is assured by the current manufacturing process (with several recommended enhancements) without the need for- testing each element in the HTF

The ATM and Rad3-Related (ATR) Protein Kinase Pathway Is Activated by Herpes Simplex Virus 1 and Required for Efficient Viral Replication.

Science.gov (United States)

Edwards, Terri G; Bloom, David C; Fisher, Chris

2018-03-15

The ATM and Rad3-related (ATR) protein kinase and its downstream effector Chk1 are key sensors and organizers of the DNA damage response (DDR) to a variety of insults. Previous studies of herpes simplex virus 1 (HSV-1) showed no evidence for activation of the ATR pathway. Here we demonstrate that both Chk1 and ATR were phosphorylated by 3 h postinfection (h.p.i.). Activation of ATR and Chk1 was observed using 4 different HSV-1 strains in multiple cell types, while a specific ATR inhibitor blocked activation. Mechanistic studies point to early viral gene expression as a key trigger for ATR activation. Both pATR and pChk1 localized to the nucleus within viral replication centers, or associated with their periphery, by 3 h.p.i. Significant levels of pATR and pChk1 were also detected in the cytoplasm, where they colocalized with ICP4 and ICP0. Proximity ligation assays confirmed that pATR and pChk1 were closely and specifically associated with ICP4 and ICP0 in both the nucleus and cytoplasm by 3 h.p.i., but not with ICP8 or ICP27, presumably in a multiprotein complex. Chemically distinct ATR and Chk1 inhibitors blocked HSV-1 replication and infectious virion production, while inhibitors of ATM, Chk2, and DNA-dependent protein kinase (DNA-PK) did not. Together our data show that HSV-1 activates the ATR pathway at early stages of infection and that ATR and Chk1 kinase activities play important roles in HSV-1 replication fitness. These findings indicate that the ATR pathway may provide insight for therapeutic approaches. IMPORTANCE Viruses have evolved complex associations with cellular DNA damage response (DDR) pathways, which sense troublesome DNA structures formed during infection. The first evidence for activation of the ATR pathway by HSV-1 is presented. ATR is activated, and its downstream target Chk1 is robustly phosphorylated, during early stages of infection. Both activated proteins are found in the nucleus associated with viral replication compartments and in

Advanced Test Reactor National Scientific User Facility (ATR NSUF) Monthly Report November 2014

Energy Technology Data Exchange (ETDEWEB)

Soelberg, Renae [Idaho National Lab. (INL), Idaho Falls, ID (United States)

2014-11-01

Advanced Test Reactor National Scientific User Facility (ATR NSUF) Monthly Report November 2014 Highlights Rory Kennedy and Sarah Robertson attended the American Nuclear Society Winter Meeting and Nuclear Technology Expo in Anaheim, California, Nov. 10-13. ATR NSUF exhibited at the technology expo where hundreds of meeting participants had an opportunity to learn more about ATR NSUF. Dr. Kennedy briefed the Nuclear Engineering Department Heads Organization (NEDHO) on the workings of the ATR NSUF. • Rory Kennedy, James Cole and Dan Ogden participated in a reactor instrumentation discussion with Jean-Francois Villard and Christopher Destouches of CEA and several members of the INL staff. • ATR NSUF received approval from the NE-20 office to start planning the annual Users Meeting. The meeting will be held at INL, June 22-25. • Mike Worley, director of the Office of Innovative Nuclear Research (NE-42), visited INL Nov. 4-5. Milestones Completed • Recommendations for the Summer Rapid Turnaround Experiment awards were submitted to DOE-HQ Nov. 12 (Level 2 milestone due Nov. 30). Major Accomplishments/Activities • The University of California, Santa Barbara 2 experiment was unloaded from the GE-2000 at HFEF. The experiment specimen packs will be removed and shipped to ORNL for PIE. • The Terrani experiment, one of three FY 2014 new awards, was completed utilizing the Advanced Photon Source MRCAT beamline. The experiment investigated the chemical state of Ag and Pd in SiC shell of irradiated TRISO particles via X-ray Absorption Fine Structure (XAFS) spectroscopy. Upcoming Meetings/Events • The ATR NSUF program review meeting will be held Dec. 9-10 at L’Enfant Plaza. In addition to NSUF staff and users, NE-4, NE-5 and NE-7 representatives will attend the meeting. Awarded Research Projects Boise State University Rapid Turnaround Experiments (14-485 and 14-486) Nanoindentation and TEM work on the T91, HT9, HCM12A and 9Cr ODS specimens has been completed at

Leak detection in the primary reactor coolant piping of nuclear power plant by applying beam-microphone technology

International Nuclear Information System (INIS)

Kasai, Yoshimitsu; Shimanskiy, Sergey; Naoi, Yosuke; Kanazawa, Junichi

2004-01-01

A microphone leak detection method was applied to the inlet piping of the ATR-prototype reactor, Fugen. Statistical analysis results showed that the cross-correlation method provided the effective results for detection of a small leakage. However, such a technique has limited application due to significant distortion of the signals on the reactor site. As one of the alternative methods, the beam-microphone provides necessary spatial selectivity and its performance is less affected by signal distortion. A prototype of the beam-microphone was developed and then tested at the O-arai Engineering Center of the Japan Nuclear Cycle Development Institute (JNC). On-site testing of the beam-microphone was carried out in the inlet piping room of an RBMK reactor of the Leningrad Nuclear Power Plant (LNPP) in Russia. A leak sound imitator was used to simulate the leakage sound under the leakage flow condition of 1-3 gpm (0.23-0.7 m 3 /h). Analysis showed that signal distortion does not seriously affect the performance of this method, and that sound reflection may result in the appearance of ghost sound sources. The test results showed that the influences of sound reflection and background noise were smaller at the high frequencies where the leakage location could be estimated with an angular accuracy of 5deg which is the range of localization accuracy required for the leak detection system. (author)

Seeded growth fabrication of Cu-on-Si electrodes for in situ ATR-SEIRAS applications

Energy Technology Data Exchange (ETDEWEB)

Wang, Hui-Feng [Shanghai Key Laboratory for Molecular Catalysis and Innovative Materials and Department of Chemistry, Fudan University, Shanghai 200433 (China); Yan, Yan-Gang [Shanghai Key Laboratory for Molecular Catalysis and Innovative Materials and Department of Chemistry, Fudan University, Shanghai 200433 (China); Huo, Sheng-Juan [Shanghai Key Laboratory for Molecular Catalysis and Innovative Materials and Department of Chemistry, Fudan University, Shanghai 200433 (China); Cai, Wen-Bin [Shanghai Key Laboratory for Molecular Catalysis and Innovative Materials and Department of Chemistry, Fudan University, Shanghai 200433 (China); Department of Enviromental Engineering, Shanghai University of Electric Power, Shanghai 200090 (China); E-mail: wbcai@fudan.edu.cn; Xu, Qun-Jie [Department of Enviromental Engineering, Shanghai University of Electric Power, Shanghai 200090 (China); Osawa, Masatoshi [Catalysis Research Center, Hokkaido University, Sapporo 001-0021 (Japan)

2007-05-25

A seeded-growth approach has been developed to fabricate a Cu nanoparticle film (simplified hereafter with nanofilm) on Si for electrochemical ATR surface-enhanced infrared absorption spectroscopy (ATR-SEIRAS). The approach comprises an initial activation of the reflecting plane of hemicylindrical Si prism by introducing a Cu seed layer in a CuSO{sub 4}-HF solution and the subsequent electroless deposition of the Cu nanofilms from an electroless Cu plating bath. The as-deposited Cu nanofilm exhibited strong SEIRA effect for the CO probe and interfacial free H{sub 2}O. ATR-SEIRAS was also applied to characterize the adsorbed geometries of pyridine at the Cu/electrolyte interface. Only vibrational bands assignable to the A {sub 1} symmetry modes were detected in the entire potential window investigated, suggestive of an end-on adsorption via the ring N-atom on a Cu electrode.

Seeded growth fabrication of Cu-on-Si electrodes for in situ ATR-SEIRAS applications

International Nuclear Information System (INIS)

Wang, Hui-Feng; Yan, Yan-Gang; Huo, Sheng-Juan; Cai, Wen-Bin; Xu, Qun-Jie; Osawa, Masatoshi

2007-01-01

A seeded-growth approach has been developed to fabricate a Cu nanoparticle film (simplified hereafter with nanofilm) on Si for electrochemical ATR surface-enhanced infrared absorption spectroscopy (ATR-SEIRAS). The approach comprises an initial activation of the reflecting plane of hemicylindrical Si prism by introducing a Cu seed layer in a CuSO 4 -HF solution and the subsequent electroless deposition of the Cu nanofilms from an electroless Cu plating bath. The as-deposited Cu nanofilm exhibited strong SEIRA effect for the CO probe and interfacial free H 2 O. ATR-SEIRAS was also applied to characterize the adsorbed geometries of pyridine at the Cu/electrolyte interface. Only vibrational bands assignable to the A 1 symmetry modes were detected in the entire potential window investigated, suggestive of an end-on adsorption via the ring N-atom on a Cu electrode

Volvulus and bowel obstruction in ATR-X syndrome-clinical report and review of literature.

Science.gov (United States)

Horesh, Nir; Pery, Ron; Amiel, Imri; Shwaartz, Chaya; Speter, Chen; Guranda, Larisa; Gutman, Mordechai; Hoffman, Aviad

2015-11-01

Alpha thalassemia-mental retardation, X-linked (ATR-X) syndrome is a rare genetic disorder with a variety of clinical manifestations. Gastrointestinal symptoms described in this syndrome include difficulties in feeding, regurgitation and vomiting which may lead to aspiration pneumonia, abdominal pain, distention, and constipation. We present a 19-year-old male diagnosed with ATR-X syndrome, who suffered from recurrent colonic volvulus that ultimately led to bowel necrosis with severe septic shock requiring emergent surgical intervention. During 1 year, the patient was readmitted four times due to poor oral intake, dehydration and abdominal distention. Investigation revealed partial small bowel volvulus which resolved with non-operative treatment. Small and large bowel volvulus are uncommon and life-threatening gastrointestinal manifestations of ATR-X patients, which may contribute to the common phenomenon of prolonged food refusal in these patients. © 2015 Wiley Periodicals, Inc.

In vitro analysis of the role of replication protein A (RPA) and RPA phosphorylation in ATR-mediated checkpoint signaling.

Science.gov (United States)

Lindsey-Boltz, Laura A; Reardon, Joyce T; Wold, Marc S; Sancar, Aziz

2012-10-19

Replication protein A (RPA) plays essential roles in DNA metabolism, including replication, checkpoint, and repair. Recently, we described an in vitro system in which the phosphorylation of human Chk1 kinase by ATR (ataxia telangiectasia mutated and Rad3-related) is dependent on RPA bound to single-stranded DNA. Here, we report that phosphorylation of other ATR targets, p53 and Rad17, has the same requirements and that RPA is also phosphorylated in this system. At high p53 or Rad17 concentrations, RPA phosphorylation is inhibited and, in this system, RPA with phosphomimetic mutations cannot support ATR kinase function, whereas a non-phosphorylatable RPA mutant exhibits full activity. Phosphorylation of these ATR substrates depends on the recruitment of ATR and the substrates by RPA to the RPA-ssDNA complex. Finally, mutant RPAs lacking checkpoint function exhibit essentially normal activity in nucleotide excision repair, revealing RPA separation of function for checkpoint and excision repair.

In Vitro Analysis of the Role of Replication Protein A (RPA) and RPA Phosphorylation in ATR-mediated Checkpoint Signaling*

Science.gov (United States)

Lindsey-Boltz, Laura A.; Reardon, Joyce T.; Wold, Marc S.; Sancar, Aziz

2012-01-01

Replication protein A (RPA) plays essential roles in DNA metabolism, including replication, checkpoint, and repair. Recently, we described an in vitro system in which the phosphorylation of human Chk1 kinase by ATR (ataxia telangiectasia mutated and Rad3-related) is dependent on RPA bound to single-stranded DNA. Here, we report that phosphorylation of other ATR targets, p53 and Rad17, has the same requirements and that RPA is also phosphorylated in this system. At high p53 or Rad17 concentrations, RPA phosphorylation is inhibited and, in this system, RPA with phosphomimetic mutations cannot support ATR kinase function, whereas a non-phosphorylatable RPA mutant exhibits full activity. Phosphorylation of these ATR substrates depends on the recruitment of ATR and the substrates by RPA to the RPA-ssDNA complex. Finally, mutant RPAs lacking checkpoint function exhibit essentially normal activity in nucleotide excision repair, revealing RPA separation of function for checkpoint and excision repair. PMID:22948311

[Relationship between PMI and ATR-FTIR Spectral Changes in Swine Costal Cartilages and Ribs].

Science.gov (United States)

Yao, Yao; Wang, Qi; Jing, Xiao-li; Li, Bing; Zhang, Yin-ming; Wang, Zhi-jun; Li, Cheng-zhi; Lin, Han-cheng; Zhang, Ji; Huang, Ping; Wang, Zhen-yuan

2016-02-01

To analyze postmortem chemical changes in Landrace costal cartilages and ribs using attenuated total reflection-Fourier transform infrared (ATR-FTIR) spectroscopy, and to provide a novel technique for estimation of postmortem interval (PMI). The swines were sacrificed by hemorrhage and their costal cartilages and ribs were kept in 20 degrees C. The chemical analysis of the costal cartilages and ribs were performed using ATR-FTIR every 72 h. The correlation between the certain spectral parameters and PMI was also analyzed. The time-dependent changes of costal cartilages were more significant than ribs. There were no obvious changes for the main absorbance bands position, and some absorbance band ratios showed time-dependent changes and significant correlations with the PMI. ATR-FTIR has the ability to analyze postmortem chemical changes of the swine costal cartilages and ribs, and it can be a new method to estimate PMI based on spectroscopy.

Analysis of H2O in silicate glass using attenuated total reflectance (ATR) micro-FTIR spectroscopy

Science.gov (United States)

Lowenstern, Jacob B.; Pitcher, Bradley W.

2013-01-01

We present a calibration for attenuated total reflectance (ATR) micro-FTIR for analysis of H2O in hydrous glass. A Ge ATR accessory was used to measure evanescent wave absorption by H2O within hydrous rhyolite and other standards. Absorbance at 3450 cm−1 (representing total H2O or H2Ot) and 1630 cm−1 (molecular H2O or H2Om) showed high correlation with measured H2O in the glasses as determined by transmission FTIR spectroscopy and manometry. For rhyolite, wt%H2O=245(±9)×A3450-0.22(±0.03) and wt%H2Om=235(±11)×A1630-0.20(±0.03) where A3450 and A1630 represent the ATR absorption at the relevant infrared wavelengths. The calibration permits determination of volatiles in singly polished glass samples with spot size down to ~5 μm (for H2O-rich samples) and detection limits of ~0.1 wt% H2O. Basaltic, basaltic andesite and dacitic glasses of known H2O concentrations fall along a density-adjusted calibration, indicating that ATR is relatively insensitive to glass composition, at least for calc-alkaline glasses. The following equation allows quantification of H2O in silicate glasses that range in composition from basalt to rhyolite: wt%H2O=(ω×A3450/ρ)+b where ω = 550 ± 21, b = −0.19 ± 0.03, ρ = density, in g/cm3, and A3450 is the ATR absorbance at 3450 cm−1. The ATR micro-FTIR technique is less sensitive than transmission FTIR, but requires only a singly polished sample for quantitative results, thus minimizing time for sample preparation. Compared with specular reflectance, it is more sensitive and better suited for imaging of H2O variations in heterogeneous samples such as melt inclusions. One drawback is that the technique can damage fragile samples and we therefore recommend mounting of unknowns in epoxy prior to polishing. Our calibration should hold for any Ge ATR crystals with the same incident angle (31°). Use of a different crystal type or geometry would require measurement of several H2O-bearing standards to provide a crystal

ATR kinase regulates its attenuation via PPM1D phosphatase ...

Indian Academy of Sciences (India)

Debadrita Bhattacharya

2018-02-07

Feb 7, 2018 ... generated in response to ultraviolet and ionizing radiation (Lu et al. 2005a, b; Cha ... nocopy' each other's effects by uncovering persistent ATR signalling that in ...... been shown to indirectly stabilize PPM1D by mediating the.

10 CFR 830 Major Modification Determination for the ATR Diesel Bus (E-3) and Switchgear Replacement

International Nuclear Information System (INIS)

Duckwitz, Noel

2011-01-01

Near term replacement of aging and obsolescent original ATR equipment has become important to ensure ATR capability in support of NE's long term national missions. To that end, a mission needs statement has been prepared for a non-major system acquisition which is comprised of three interdependent subprojects. The first project, subject of this determination, will replace the existent diesel-electrical bus (E-3) and associated switchgear. More specifically, INL proposes transitioning ATR to 100% commercial power with appropriate emergency backup to include: (1) Provide commercial power as the normal source of power to the ATR loads currently supplied by diesel-electric power. (2) Provide backup power to the critical ATR loads in the event of a loss of commercial power. (3) Replace obsolescent critical ATR power distribution equipment, e.g., switchgear, transformers, motor control centers, distribution panels. Completion of this and two other age-related projects (primary coolant pump and motor replacement and emergency firewater injection system replacement) will resolve major age related operational issues plus make a significant contribution in sustaining the ATR safety and reliability profile. The major modification criteria evaluation of the project pre-conceptual design identified several issues make the project a major modification: (1) Evaluation Criteria No.2 (Footprint change). The addition of a new PC-4 structure to the ATR Facility to house safety-related SSCs requires careful attention to maintaining adherence to applicable engineering and nuclear safety design criteria (e.g., structural qualification, fire suppression) to ensure no adverse impacts to the safety-related functions of the housed equipment. (2) Evaluation Criteria No.3 (Change of existing process). The change to the strategy for providing continuous reliable power to the safety-related emergency coolant pumps requires careful attention and analysis to ensure it meets a project primary

Analysis of cosmetic residues on a single human hair by ATR FT-IR microspectroscopy.

Science.gov (United States)

Pienpinijtham, Prompong; Thammacharoen, Chuchaat; Naranitad, Suwimol; Ekgasit, Sanong

2018-05-15

In this work, ATR FT-IR spectra of single human hair and cosmetic residues on hair surface are successfully collected using a homemade dome-shaped Ge μIRE accessary installed on an infrared microscope. By collecting ATR spectra of hairs from the same person, the spectral patterns are identical and superimposed while different spectral features are observed from ATR spectra of hairs collected from different persons. The spectral differences depend on individual hair characteristics, chemical treatments, and cosmetics on hair surface. The "Contact-and-Collect" technique that transfers remarkable materials on the hair surface to the tip of the Ge μIRE enables an identification of cosmetics on a single hair. Moreover, the differences between un-split and split hairs are also studied in this report. These highly specific spectral features can be employed for unique identification or for differentiation of hairs based on the molecular structures of hairs and cosmetics on hairs. Copyright © 2018 Elsevier B.V. All rights reserved.

Analysis of cosmetic residues on a single human hair by ATR FT-IR microspectroscopy

Science.gov (United States)

Pienpinijtham, Prompong; Thammacharoen, Chuchaat; Naranitad, Suwimol; Ekgasit, Sanong

2018-05-01

In this work, ATR FT-IR spectra of single human hair and cosmetic residues on hair surface are successfully collected using a homemade dome-shaped Ge μIRE accessary installed on an infrared microscope. By collecting ATR spectra of hairs from the same person, the spectral patterns are identical and superimposed while different spectral features are observed from ATR spectra of hairs collected from different persons. The spectral differences depend on individual hair characteristics, chemical treatments, and cosmetics on hair surface. The "Contact-and-Collect" technique that transfers remarkable materials on the hair surface to the tip of the Ge μIRE enables an identification of cosmetics on a single hair. Moreover, the differences between un-split and split hairs are also studied in this report. These highly specific spectral features can be employed for unique identification or for differentiation of hairs based on the molecular structures of hairs and cosmetics on hairs.

Gauvain e il corpo smembrato: allegorie nell'«Atre périlleux»

Directory of Open Access Journals (Sweden)

Maurizio Virdis

2013-07-01

Full Text Available L’Atre Périlleux è un romanzo anonimo francese del XIII secolo e fa parte della corrente letteraria che rimettere in questione il romanzo cortese di tradizione chrestieniana. Piú che all’ideologia o alle istanze della classe cavalleresca e della nobiltà minore, il nostro romanzo risponde alle prospettive e alle necessità intellettuali della clergie, che si fa gioco dei valori cavallereschi tradizionali. L’Atre Périlleux è condotto, con toni di humour e una trama complessa, su binari metaletterari e allegorici (di cui il corpo smembrato di Gauvain è il simbolo piú eminente, e si pone come critica della decadenza della società cortese, animato però dal proposito del rinnovamento morale di essa.L’Atre Périlleux is an anonymous French romance of the thirteenth century and it is part of the literary movement that calls into question the tradition of courtly romance dating back to Chrétien de Troyes. More than to the ideology and instances of the class of minor nobility and chivalry, our romance responds to the clergie’s needs and intellectual perspectives, and it mocks the traditional values of chivalry. L’Atre Périlleux is conducted, with a hint of humour and a complicated plot, on metaliterary and allegorical tracks (and Gauvain’s dismembered body is the most prominent symbol of this allegorical tone, so that our romance stands as a critique of courtly society decadence, nevertheless animated by the purpose of its moral renewal.

ATR- and ATM-Mediated DNA Damage Response Is Dependent on Excision Repair Assembly during G1 but Not in S Phase of Cell Cycle.

Science.gov (United States)

Ray, Alo; Blevins, Chessica; Wani, Gulzar; Wani, Altaf A

2016-01-01

Cell cycle checkpoint is mediated by ATR and ATM kinases, as a prompt early response to a variety of DNA insults, and culminates in a highly orchestrated signal transduction cascade. Previously, we defined the regulatory role of nucleotide excision repair (NER) factors, DDB2 and XPC, in checkpoint and ATR/ATM-dependent repair pathway via ATR and ATM phosphorylation and recruitment to ultraviolet radiation (UVR)-induced damage sites. Here, we have dissected the molecular mechanisms of DDB2- and XPC- mediated regulation of ATR and ATM recruitment and activation upon UVR exposures. We show that the ATR and ATM activation and accumulation to UVR-induced damage not only depends on DDB2 and XPC, but also on the NER protein XPA, suggesting that the assembly of an active NER complex is essential for ATR and ATM recruitment. ATR and ATM localization and H2AX phosphorylation at the lesion sites occur as early as ten minutes in asynchronous as well as G1 arrested cells, showing that repair and checkpoint-mediated by ATR and ATM starts early upon UV irradiation. Moreover, our results demonstrated that ATR and ATM recruitment and H2AX phosphorylation are dependent on NER proteins in G1 phase, but not in S phase. We reasoned that in G1 the UVR-induced ssDNA gaps or processed ssDNA, and the bound NER complex promote ATR and ATM recruitment. In S phase, when the UV lesions result in stalled replication forks with long single-stranded DNA, ATR and ATM recruitment to these sites is regulated by different sets of proteins. Taken together, these results provide evidence that UVR-induced ATR and ATM recruitment and activation differ in G1 and S phases due to the existence of distinct types of DNA lesions, which promote assembly of different proteins involved in the process of DNA repair and checkpoint activation.

The Conference Proceedings of the 2001 Air Transport Research Society (ATRS) of the WCTR Society. Volume 2

Science.gov (United States)

Lee, Yeong-Heok (Editor); Bowen, Brent D. (Editor); Tarry, Scott E. (Editor)

2001-01-01

The ATRS held its 5th Annual conference at the City University of Hong Kong Campus in July 2001. The conference was a success with nearly 140 participants including 70 presenters. Titles that comprise Volume 2 include: Intelligent Airport Gate Assignment System; A Study on the Effects of the Personality Compatibility to the Job Performance; ITS/CVO Application for Air cargo Transportation in Korea; An Airport as a Logistics and Economic Hub: The Case of Incheon International Airport; The Impact Of Aviation Safety over the Consumer's Behavior; The Integration of China and Taiwan Air Networks for Direct Air Cargo Services; Quality perception and carrier choice in Civil Aviation; Future Trends in Business Travel Decision Making; Cooperation Among German Airports in Europe; Inbound and Outbound Air Passenger Traffic Forecasting between the United States and Selected Asian countries; An Evaluation of Alternative Facilities for Airport Redevelopment using Fuzzy Linguistic Approach; Economic Analysis of Airline Alliances; The Aviation Cooperation between the two Koreas Preparing for the Reunification of the Peninsula; and A Study on the Air Transport Cooperation in Northeast Asia between China, Japan and Korea.

Multivariate analysis of ATR-FTIR spectra for assessment of oil shale organic geochemical properties

Science.gov (United States)

Washburn, Kathryn E.; Birdwell, Justin E.

2013-01-01

In this study, attenuated total reflectance (ATR) Fourier transform infrared spectroscopy (FTIR) was coupled with partial least squares regression (PLSR) analysis to relate spectral data to parameters from total organic carbon (TOC) analysis and programmed pyrolysis to assess the feasibility of developing predictive models to estimate important organic geochemical parameters. The advantage of ATR-FTIR over traditional analytical methods is that source rocks can be analyzed in the laboratory or field in seconds, facilitating more rapid and thorough screening than would be possible using other tools. ATR-FTIR spectra, TOC concentrations and Rock–Eval parameters were measured for a set of oil shales from deposits around the world and several pyrolyzed oil shale samples. PLSR models were developed to predict the measured geochemical parameters from infrared spectra. Application of the resulting models to a set of test spectra excluded from the training set generated accurate predictions of TOC and most Rock–Eval parameters. The critical region of the infrared spectrum for assessing S1, S2, Hydrogen Index and TOC consisted of aliphatic organic moieties (2800–3000 cm−1) and the models generated a better correlation with measured values of TOC and S2 than did integrated aliphatic peak areas. The results suggest that combining ATR-FTIR with PLSR is a reliable approach for estimating useful geochemical parameters of oil shales that is faster and requires less sample preparation than current screening methods.

A review of fast reactor program in Japan

International Nuclear Information System (INIS)

1992-01-01

full efficient usage of the test results. R and D on following tasks are also in progress for development of the DFBR, for excellent technology to attain FBR commercialization, and for technological breakthrough: development of advanced fuels; development of advanced large core; higher plant operating temperature; simplified advanced piping and components; development of rational confinement facilities; develop rent of seismic isolation structures; development of simplified system without secondary loops; development of highly reliable decay-heat removal system; development of advanced operational and maintenance technology; establishment of rational safety logic. In addition to the MOX fuel fabrication at the Plutonium Fuel Fabrication Facility for Joyo, Fugen (AIR), and BWRs in Japan, a new Plutonium Fuel Production Facility (PFPF) was constructed at Tokai Works of PNC and MOX fuel production for Monju is in progress. On the FBR fuel recycling, adding to the experiences at the Tokai Reprocessing Plant, R and D are underway at three Engineering Demonstration Facilities (EDF-I, II, and III) and Chemical Processing Facility (CPF), integrating the results to the design of Recycling Equipment Test Facility (RETF) and future FBR Fuel Recycling Pilot Plant. Following the national program on waste management, PNC is also actively contributing to the area of vitrification of high level liquid waste, geological disposal of it, and low level transuranium bearing waste treatment, and promotion of construction of a storage engineering center in Hokkaido. Aiming to the age of future FBR commercialization, further extensive and effective collaboration with foreign institutions will also have to play an important role

A review of fast reactor program in Japan

Energy Technology Data Exchange (ETDEWEB)

NONE

1992-07-01

full efficient usage of the test results. R and D on following tasks are also in progress for development of the DFBR, for excellent technology to attain FBR commercialization, and for technological breakthrough: development of advanced fuels; development of advanced large core; higher plant operating temperature; simplified advanced piping and components; development of rational confinement facilities; develop rent of seismic isolation structures; development of simplified system without secondary loops; development of highly reliable decay-heat removal system; development of advanced operational and maintenance technology; establishment of rational safety logic. In addition to the MOX fuel fabrication at the Plutonium Fuel Fabrication Facility for Joyo, Fugen (AIR), and BWRs in Japan, a new Plutonium Fuel Production Facility (PFPF) was constructed at Tokai Works of PNC and MOX fuel production for Monju is in progress. On the FBR fuel recycling, adding to the experiences at the Tokai Reprocessing Plant, R and D are underway at three Engineering Demonstration Facilities (EDF-I, II, and III) and Chemical Processing Facility (CPF), integrating the results to the design of Recycling Equipment Test Facility (RETF) and future FBR Fuel Recycling Pilot Plant. Following the national program on waste management, PNC is also actively contributing to the area of vitrification of high level liquid waste, geological disposal of it, and low level transuranium bearing waste treatment, and promotion of construction of a storage engineering center in Hokkaido. Aiming to the age of future FBR commercialization, further extensive and effective collaboration with foreign institutions will also have to play an important r0008.

Measured Thermal and Fast Neutron Fluence Rates for ATF-1 Holders During ATR Cycle 157D

Energy Technology Data Exchange (ETDEWEB)

Smith, Larry Don [Idaho National Lab. (INL), Idaho Falls, ID (United States); Miller, David Torbet [Idaho National Lab. (INL), Idaho Falls, ID (United States)

2016-03-01

This report contains the thermal (2200 m/s) and fast (E>1MeV) neutron fluence rate data for the ATF-1 holders located in core for ATR Cycle 157D which were measured by the Radiation Measurements Laboratory (RML) as requested by the Power Reactor Programs (ATR Experiments) Radiation Measurements Work Order. This report contains measurements of the fluence rates corresponding to the particular elevations relative to the 80-ft. core elevation. The data in this report consist of (1) a table of the ATR power history and distribution, (2) a hard copy listing of all thermal and fast neutron fluence rates, and (3) plots of both the thermal and fast neutron fluence rates. The fluence rates reported are for the average power levels given in the table of power history and distribution.

In situ detection of cancerous kidney tissue by means of fiber ATR-FTIR spectroscopy

Science.gov (United States)

Sablinskas, Valdas; Velicka, Martynas; Pucetaite, Milda; Urboniene, Vidita; Ceponkus, Justinas; Bandzeviciute, Rimante; Jankevicius, Feliksas; Sakharova, Tatiana; Bibikova, Olga; Steiner, Gerald

2018-02-01

The crucial goal of kidney-sparing surgical resection of a malignant tumor is complete removal of the cancerous tissue. The exact border between the cancerous and normal tissues is not always possible to identify by naked eye, therefore, a supplementary intraoperative diagnosis is needed. Unfortunately, intraoperative pathology methods used nowadays are time consuming and of inadequate quality rendering not definitive diagnosis. It has recently been shown that ATR-FTIR spectroscopy can be used for fast discrimination between cancerous and normal kidney tissues by analyzing the collected spectra of the tissue touch imprint smears. Most prominent differences are obtained in the wavenumber region from 950 cm-1 to 1250 cm-1, where the spectral bands due to the molecular vibrations of glycogen arise in the spectra of cancerous tissue smears. Such method of detection of cancerous tissue is limited by requirement to transfer the suspected tissue from the body to the FTIR instrument and stamp it on an ATR crystal of the spectrometer. We propose a spectroscopic tool which exploits the same principle of detection of cancerous cells as mentioned above, but does not require the tissue to be transferred from the body to the spectrometer. The portable spectrometer used in this design is equipped with fiber ATR probe and a sensitive liquid nitrogen cooled MCT detector. The design of the fiber probe allows the ATR tip to be changed easily in order to use only new sterilized tips for each measurement point of the tissue. It also enables sampling multiple areas of the suspected tissue with high lateral resolution which, in turn, increases accuracy with which the marginal regions between normal and cancerous tissues can be identified. Due to the loss of optical signal in the fiber probe the spectra have lower signal-to-noise ratio than in the case of standard ATR sampling setup. However, software for the spectral analysis used with the fiber probe design is still able to distinguish

Achieving safety/risk goals for less ATR backup power upgrades

International Nuclear Information System (INIS)

Atkinson, S.A.

1995-01-01

The Advanced Test Reactor probabilistic risk assessment for internal fire and flood events defined a relatively high risk for a total loss of electric power possibly leading to core damage. Backup power sources were disabled due to fire and flooding in the diesel generator area with propagation of the flooding to a common switchgear room. The ATR risk assessment was employed to define options for relocation of backup power system components to achieve needed risk reduction while minimizing costs. The risk evaluations were performed using sensitivity studies and importance measures. The risk-based evaluations of relocation options for backup power systems saved over $3 million from what might have been otherwise considered open-quotes necessaryclose quotes for safety/risk improvement. The ATR experience shows that the advantages of a good risk assessment are to define risk significance, risk specifics, and risk solutions which enable risk goals to be achieved at the lowest cost

Diamond-coated ATR prism for infrared absorption spectroscopy of surface-modified diamond nanoparticles

Energy Technology Data Exchange (ETDEWEB)

Remes, Z., E-mail: remes@fzu.cz [Institute of Physics of the ASCR, v.v.i., Cukrovarnicka 10, Praha 6 (Czech Republic); Kozak, H.; Rezek, B.; Ukraintsev, E.; Babchenko, O.; Kromka, A. [Institute of Physics of the ASCR, v.v.i., Cukrovarnicka 10, Praha 6 (Czech Republic); Girard, H.A.; Arnault, J.-C.; Bergonzo, P. [CEA, LIST, Diamond Sensors Laboratory, F-91191 Gif-sur-Yvette (France)

2013-04-01

Linear antenna microwave chemical vapor deposition process was used to homogeneously coat a 7 cm long silicon prism by 85 nm thin nanocrystalline diamond (NCD) layer. To show the advantages of the NCD-coated prism for attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR) of nanoparticles, we apply diamond nanoparticles (DNPs) of 5 nm nominal size with various surface modifications by a drop-casting of their methanol dispersions. ATR-FTIR spectra of as-received, air-annealed, plasma-oxidized, and plasma-hydrogenated DNPs were measured in the 4000–1500 cm{sup −1} spectral range. The spectra show high spectral resolution, high sensitivity to specific DNP surface moieties, and repeatability. The NCD coating provides mechanical protection against scratching and chemical stability of the surface. Moreover, unlike on bare Si surface, NCD hydrophilic properties enable optically homogeneous coverage by DNPs with some aggregation on submicron scale as evidenced by scanning electron microscopy and atomic force microscopy. Compared to transmission FTIR regime with KBr pellets, direct and uniform deposition of DNPs on NCD-ATR prism significantly simplifies and speeds up the analysis (from days to minutes). We discuss prospects for in situ monitoring of surface modifications and molecular grafting.

Diamond-coated ATR prism for infrared absorption spectroscopy of surface-modified diamond nanoparticles

Science.gov (United States)

Remes, Z.; Kozak, H.; Rezek, B.; Ukraintsev, E.; Babchenko, O.; Kromka, A.; Girard, H. A.; Arnault, J.-C.; Bergonzo, P.

2013-04-01

Linear antenna microwave chemical vapor deposition process was used to homogeneously coat a 7 cm long silicon prism by 85 nm thin nanocrystalline diamond (NCD) layer. To show the advantages of the NCD-coated prism for attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR) of nanoparticles, we apply diamond nanoparticles (DNPs) of 5 nm nominal size with various surface modifications by a drop-casting of their methanol dispersions. ATR-FTIR spectra of as-received, air-annealed, plasma-oxidized, and plasma-hydrogenated DNPs were measured in the 4000-1500 cm-1 spectral range. The spectra show high spectral resolution, high sensitivity to specific DNP surface moieties, and repeatability. The NCD coating provides mechanical protection against scratching and chemical stability of the surface. Moreover, unlike on bare Si surface, NCD hydrophilic properties enable optically homogeneous coverage by DNPs with some aggregation on submicron scale as evidenced by scanning electron microscopy and atomic force microscopy. Compared to transmission FTIR regime with KBr pellets, direct and uniform deposition of DNPs on NCD-ATR prism significantly simplifies and speeds up the analysis (from days to minutes). We discuss prospects for in situ monitoring of surface modifications and molecular grafting.

In Vivo and In Vitro Effects of ATM/ATR Signaling Pathway on Proliferation, Apoptosis, and Radiosensitivity of Nasopharyngeal Carcinoma Cells.

Science.gov (United States)

Wang, Ming; Liu, Gang; Shan, Guo-Ping; Wang, Bing-Bing

2017-08-01

The study investigated the ability of ataxia-telangiectasia mutated (ATM)/Rad3-related (ATR) signaling pathway to influence the proliferation, apoptosis, and radiosensitivity of nasopharyngeal carcinoma (NPC) cells. NPC tissues and corresponding adjacent normal tissues were collected from 143 NPC patients. The NPC CNE2 cells were assigned into a control group, X-ray group, CGK-733 group, and X-ray+CGK-733 group. The mRNA levels of ATM and ATR were evaluated using quantitative real-time polymerase chain reaction (qRT-PCR) and the protein levels of ATM and ATR using western blotting. The positive expression of ATM and ATR in tissues and nude mouse tumor tissues was determined by immunohistochemistry. Cell proliferation, migration, invasion, and apoptosis rates were analyzed by the 3-(4,5)-dimethylthiahiazo (-z-y1)-3,5-di- phenytetrazoliumromide (MTT) assay, scratch test, transwell assay, and flow cytometry, respectively. A nude mouse model of NPC was established to observe tumor volume and growth. The mRNA levels of ATR and ATM and the expression of ATR and ATM protein in NPC tissues were significantly higher than those in adjacent normal tissues. The colony formation assay showed that the colony-forming rate decreased, showing radiation dose-dependent and CGK-733 concentration-dependent manners. Expression of ATM, ATR, Chk1, and Chk2 was evidently increased in the X-ray, CGK-733, and X-ray+CGK-733groups compared with the control group, and the aforementioned expression was highest in the X-ray+CGK-733 group among the four groups. The cell proliferation, invasion, and migration were decreased, tumor volume decreased and cell apoptosis increased in the X-ray, CGK-733, and X-ray+CGK-733 groups compared with the control group; the X-ray+CGK-733 group exhibited lowest cell proliferation, invasion and migration, smallest tumor volume, and highest cell apoptosis among the four groups. Inhibition of ATM/ATR signaling pathway reduces proliferation and enhances apoptosis and

30 CFR 75.209 - Automated Temporary Roof Support (ATRS) systems.

Science.gov (United States)

2010-07-01

... of temporary support shall be used, as specified in the roof control plan, when— (1) Mining... 30 Mineral Resources 1 2010-07-01 2010-07-01 false Automated Temporary Roof Support (ATRS) systems... COAL MINE SAFETY AND HEALTH MANDATORY SAFETY STANDARDS-UNDERGROUND COAL MINES Roof Support § 75.209...

Mutation analysis and characterization of ATR sequence variants in breast cancer cases from high-risk French Canadian breast/ovarian cancer families

Directory of Open Access Journals (Sweden)

Pichette Roxane

2006-09-01

Full Text Available Abstract Background Ataxia telangiectasia-mutated and Rad3-related (ATR is a member of the PIK-related family which plays, along with ATM, a central role in cell-cycle regulation. ATR has been shown to phosphorylate several tumor suppressors like BRCA1, CHEK1 and TP53. ATR appears as a good candidate breast cancer susceptibility gene and the current study was designed to screen for ATR germline mutations potentially involved in breast cancer predisposition. Methods ATR direct sequencing was performed using a fluorescent method while widely available programs were used for linkage disequilibrium (LD, haplotype analyses, and tagging SNP (tSNP identification. Expression analyses were carried out using real-time PCR. Results The complete sequence of all exons and flanking intronic sequences were analyzed in DNA samples from 54 individuals affected with breast cancer from non-BRCA1/2 high-risk French Canadian breast/ovarian families. Although no germline mutation has been identified in the coding region, we identified 41 sequence variants, including 16 coding variants, 3 of which are not reported in public databases. SNP haplotypes were established and tSNPs were identified in 73 healthy unrelated French Canadians, providing a valuable tool for further association studies involving the ATR gene, using large cohorts. Our analyses led to the identification of two novel alternative splice transcripts. In contrast to the transcript generated by an alternative splicing site in the intron 41, the one resulting from a deletion of 121 nucleotides in exon 33 is widely expressed, at significant but relatively low levels, in both normal and tumoral cells including normal breast and ovarian tissue. Conclusion Although no deleterious mutations were identified in the ATR gene, the current study provides an haplotype analysis of the ATR gene polymorphisms, which allowed the identification of a set of SNPs that could be used as tSNPs for large-scale association

Evaluation of [18F]-ATRi as PET tracer for in vivo imaging of ATR in mouse models of brain cancer

International Nuclear Information System (INIS)

Carlucci, Giuseppe; Carney, Brandon; Sadique, Ahmad; Vansteene, Axel; Tang, Jun; Reiner, Thomas

2017-01-01

Rationale: Ataxia telangiectasia and Rad3-related (ATR) threonine serine kinase is one of the key elements in orchestrating the DNA damage response (DDR). As such, inhibition of ATR can amplify the effects of chemo- and radiation-therapy, and several ATR inhibitors (ATRi) have already undergone clinical testing in cancer. For more accurate patient selection, monitoring and staging, real-time in vivo imaging of ATR could be invaluable; the development of appropriate imaging agents has remained a major challenge. Methods: 3-amino-N-(4-[ 18 F]phenyl)-6-(4-(methylsulfonyl)phenyl) pyrazine-2-carboxamide ([ 18 F]-ATRi), a close analogue of Ve-821, (a clinical ATRi candidate), was readily accomplished similarly to already established synthetic procedures. Structurally, 18 F was introduced at the 4-position of the aromatic ring of Ve-821 for generating a labeled ATR inhibitor. In vitro experiments were conducted in U251 MG glioblastoma cell lines and ex vivo biodistribution were performed in subcutaneous U251 MG xenograft bearing athymic nude mice following microPET imaging. Results: [ 18 F]-ATRi has a similar pharmacokinetic profile to that of Ve-821. Using an U251 MG glioblastoma mouse model, we evaluated the in vivo binding efficiency of [ 18 F]-ATRi. Blood and tumor showed a statistically significant difference between mice injected with only the probe or following blocking experiment with Ve-821 (1.48 ± 0.40%ID/g vs. 0.46 ± 0.12%ID/g in tumor and 1.85 ± 0.47%ID/g vs. 0.84 ± 0.3%ID/g in blood respectively). Conclusions: [ 18 F]-ATRi represents the first 18 F positron emission tomography (PET) ATR imaging agent, and is designed on a low nanomolar and clinically relevant ATR inhibitor.

ATR-101, a selective ACAT1 inhibitor, decreases ACTH-stimulated cortisol concentrations in dogs with naturally occurring Cushing's syndrome.

Science.gov (United States)

Langlois, Daniel K; Fritz, Michele C; Schall, William D; Bari Olivier, N; Smedley, Rebecca C; Pearson, Paul G; Bailie, Marc B; Hunt, Stephen W

2018-05-02

Cushing's syndrome in humans shares many similarities with its counterpart in dogs in terms of etiology (pituitary versus adrenal causes), clinical signs, and pathophysiologic sequelae. In both species, treatment of pituitary- and adrenal-dependent disease is met with limitations. ATR-101, a selective inhibitor of ACAT1 (acyl coenzyme A:cholesterol acyltransferase 1), is a novel small molecule therapeutic currently in clinical development for the treatment of adrenocortical carcinoma, congenital adrenal hyperplasia, and Cushing's syndrome in humans. Previous studies in healthy dogs have shown that ATR-101 treatment led to rapid, dose-dependent decreases in adrenocorticotropic hormone (ACTH) stimulated cortisol levels. The purpose of this clinical study was to investigate the effects of ATR-101 in dogs with Cushing's syndrome. ATR-101 pharmacokinetics and activity were assessed in 10 dogs with naturally-occurring Cushing's syndrome, including 7 dogs with pituitary-dependent disease and 3 dogs with adrenal-dependent disease. ATR-101 was administered at 3 mg/kg PO once daily for one week, followed by 30 mg/kg PO once daily for one (n = 4) or three (n = 6) weeks. Clinical, biochemical, adrenal hormonal, and pharmacokinetic data were obtained weekly for study duration. ATR-101 exposure increased with increasing dose. ACTH-stimulated cortisol concentrations, the primary endpoint for the study, were significantly decreased with responders (9 of 10 dogs) experiencing a mean ± standard deviation reduction in cortisol levels of 50 ± 17% at study completion. Decreases in pre-ACTH-stimulated cortisol concentrations were observed in some dogs although overall changes in pre-ACTH cortisol concentrations were not significant. The compound was well-tolerated and no serious drug-related adverse effects were reported. This study highlights the potential utility of naturally occurring canine Cushing's syndrome as a model for human disease and provides proof of

Open source tools for ATR development and performance evaluation

Science.gov (United States)

Baumann, James M.; Dilsavor, Ronald L.; Stubbles, James; Mossing, John C.

2002-07-01

Early in almost every engineering project, a decision must be made about tools; should I buy off-the-shelf tools or should I develop my own. Either choice can involve significant cost and risk. Off-the-shelf tools may be readily available, but they can be expensive to purchase and to maintain licenses, and may not be flexible enough to satisfy all project requirements. On the other hand, developing new tools permits great flexibility, but it can be time- (and budget-) consuming, and the end product still may not work as intended. Open source software has the advantages of both approaches without many of the pitfalls. This paper examines the concept of open source software, including its history, unique culture, and informal yet closely followed conventions. These characteristics influence the quality and quantity of software available, and ultimately its suitability for serious ATR development work. We give an example where Python, an open source scripting language, and OpenEV, a viewing and analysis tool for geospatial data, have been incorporated into ATR performance evaluation projects. While this case highlights the successful use of open source tools, we also offer important insight into risks associated with this approach.

Radioresistance of chordoma cells is associated with the ATM/ATR pathway, in which RAD51 serves as an important downstream effector.

Science.gov (United States)

Zhang, Chao; Wang, Bing; Li, Lei; Li, Yawei; Li, Pengzhi; Lv, Guohua

2017-09-01

Surgery followed by radiotherapy is the standard treatment for chordomas, which are a rare but low-grade type of bone cancer arising from remnants of the embryonic notochord. However, disease recurrence following radiotherapy is common, most likely due to endogenous DNA repair mechanisms that promote cell survival upon radiation strikes. The ataxia telangiectasia mutated/ataxia telangiectasia mutated and Rad3 related (ATM/ATR)-mediated pathway has a critical role in DNA repair mechanisms; however, it has rarely been investigated in chordomas. In the present study, the expression of signal molecules related to the ATM/ATR pathway in chordoma tissues and adjacent normal tissues were initially examined using immunohistochemistry and western blot analysis. Chordoma U-CH1 and U-CH2 cells were subsequently used to investigate cell responses to ionizing radiation and the potential protective actions mediated by the ATM/ATR pathway. Phosphorylated (p)-ATM, p-ATR, γ-H2A histone family, member X (H2AX) and RAD51 were significantly upregulated in chordoma tissues relative to adjacent normal tissues (PATM, γ-H2AX and RAD51 expression in U-CH1 cells (PATM, p-ATR and RAD51 levels in U-CH2 cells (PATM/ATR pathway, in which RAD51 serves as an important downstream effector. Thus, RAD51 presents a promising therapeutic target for improving the outcome of radiotherapy treatment in chordomas.

Inspection of the UO2 special fuel for the prototype heavy water reactor 'FUGEN'

International Nuclear Information System (INIS)

Miura, Makoto; Ohmori, Takuro; Yoshino, Hiroyuki; Matsui, Hiromasa; Hirosawa, Naonori

1979-01-01

UO 2 special fuel assemblies are the fuel for material irradiation incorporating irradiation specimens, for the prototype heavy water reactor ''FUGEN''. In order to monitor the behavior of the pressure tube material irradiated with neutrons for long time, monitoring specimens were equipped in the core. This special fuel was fabricated by the Nuclear Fuel Industries, Ltd. (NFI), and the fuel cladding tubes, the capsule guide tubes and the capsule tubes were furnished by PNC. The irradiation specimens were prepared by PNC, and incorporated into the assemblies by NFI. The inspection by PNC on the special fuel assemblies was conducted following the inspection by the maker, which was made on UO 2 pellets, fuel element and assembly parts except cladding tubes, after completing the fabrication. The specifications of the special fuel, especially for the outer and inner layer pellets, the outer and inner layer fuel elements and the fuel assemblies, are presented. The flow sheet for the inspection process and surveillance test of special fuel assemblies is illustrated. The inspection items, the materials and the quantity inspection are tabulated for the fuel elements, the fuel assemblies and the irradiation capsules, respectively. The structure of a special type fuel assembly is shown. For each inspection, the inspection methods and items and the results are explained. As for the results of inspection of the special fuel, the UO 2 pellets, fuel element parts, fuel elements, fuel assembly parts, fuel assemblies, capsules and irradiation specimens were in accordance with the specifications. Regarding the situation of the quality control in the processes, check was made with many documents, and it was recognized that the quality control was performed in the quality assurance program. (Nakai, Y.)

VE-821, an ATR inhibitor, causes radiosensitization in human tumor cells irradiated with high LET radiation

International Nuclear Information System (INIS)

Fujisawa, Hiroshi; Nakajima, Nakako Izumi; Sunada, Shigeaki; Lee, Younghyun; Hirakawa, Hirokazu; Yajima, Hirohiko; Fujimori, Akira; Uesaka, Mitsuru; Okayasu, Ryuichi

2015-01-01

High linear energy transfer (LET) radiation such as carbon ion particles is successfully used for treatment of solid tumors. The reason why high LET radiation accomplishes greater tumor-killing than X-rays is still not completely understood. One factor would be the clustered or complex-type DNA damages. We previously reported that complex DNA double-strand breaks produced by high LET radiation enhanced DNA end resection, and this could lead to higher kinase activity of ATR protein recruited to RPA-coated single-stranded DNA. Although the effect of ATR inhibition on cells exposed to low LET gamma-rays has recently been reported, little is known regarding the effect of ATR inhibitor on cells treated with high LET radiation. The purpose of this study is to investigate the effects of the ATR inhibitor VE-821 in human tumor and normal cells irradiated with high LET carbon ions. HeLa, U2OS, and 1BR-hTERT (normal) cells were pre-treated with 1 μM VE-821 for 1 hour and irradiated with either high LET carbon ions or X-rays. Cell survival, cell cycle distribution, cell growth, and micronuclei formation were evaluated. VE-821 caused abrogation of G2/M checkpoint and forced irradiated cells to divide into daughter cells. We also found that carbon ions caused a higher number of multiple micronuclei than X-rays, leading to decreased cell survival in tumor cells when treated with VE-821, while the survival of irradiated normal cells were not significantly affected by this inhibitor. ATR inhibitor would be an effective tumor radiosensitizer with carbon ion irradiation. The online version of this article (doi:10.1186/s13014-015-0464-y) contains supplementary material, which is available to authorized users

Inherited germline ATRX mutation in two brothers with ATR-X syndrome and osteosarcoma.

Science.gov (United States)

Ji, Jianling; Quindipan, Catherine; Parham, David; Shen, Lishuang; Ruble, David; Bootwalla, Moiz; Maglinte, Dennis T; Gai, Xiaowu; Saitta, Sulagna C; Biegel, Jaclyn A; Mascarenhas, Leo

2017-05-01

We report a family in which two brothers had an undiagnosed genetic disorder comprised of dysmorphic features, microcephaly, severe intellectual disability (non-verbal), mild anemia, and cryptorchidism. Both developed osteosarcoma. Trio exome sequencing (using blood samples from the younger brother and both parents) was performed and a nonsense NM_000489.4:c.7156C>T (p.Arg2386*) mutation in the ATRX gene was identified in the proband (hemizygous) and in the mother's peripheral blood DNA (heterozygous). The mother is healthy, does not exhibit any clinical manifestations of ATR-X syndrome and there was no family history of cancer. The same hemizygous pathogenic variant was confirmed in the affected older brother's skin tissue by subsequent Sanger sequencing. Chromosomal microarray studies of both brothers' osteosarcomas revealed complex copy number alterations consistent with the clinical diagnosis of osteosarcoma. Recently, somatic mutations in the ATRX gene have been observed as recurrent alterations in both osteosarcoma and brain tumors. However, it is unclear if there is any association between osteosarcoma and germline ATRX mutations, specifically in patients with constitutional ATR-X syndrome. This is the first report of osteosarcoma diagnosed in two males with ATR-X syndrome, suggesting a potential increased risk for cancer in patients with this disorder. © 2017 Wiley Periodicals, Inc.

Advanced analysis technology for MOX fuel

International Nuclear Information System (INIS)

Hiyama, T.; Kamimura, K.

1997-01-01

PNC has developed MOX fuels for advanced thermal reactor (ATR) and fast breeder reactor (FBR). The MOX samples have been chemically analysed to characterize the MOX fuel for JOYO, MONJU, FUGEN and so on. The analysis of the MOX samples in glove box has required complicated and highly skilled operations. Therefore, for quality control analysis of the MOX fuel in a fabrication plant, simple, rapid and accurate analysis methods are necessary. To solve the above problems instrumental analysis and techniques were developed. This paper describes some of the recent developments in PNC. 2. Outline of recently developed analysis methods by PNC. 2.1 Determination of oxygen to metal atomic ratio (O/M) in MOX by non-dispersive infrared spectrophotometry after inert gas fusion. 7 refs, 9 figs, 4 tabs

Measured thermal and fast neutron fluence rates ATR Cycle 101-B, October 11, 1993--November 27, 1993

International Nuclear Information System (INIS)

Murray, R.K.; Rogers, J.W.

1994-01-01

This report contains the thermal (2200 m/s) and fast (E>lMeV) neutron fluence rate data for ATR Cycle 101-B which were measured by the Radiation Measurements Laboratory (RML) as requested by the Power Reactor Programs (ATR Experiments) Radiation Measurements Work Order. This report contains fluence rate values corresponding to the particular elevations (relative to the 80 ft. core elevation) where the measurements were taken. The data in this report consists of (1) a table of the ATR power history and distribution, (2) a hard copy listing of all thermal and fast neutron fluence rates, (3) plots of both the thermal and fast neutron fluence rates, and (4) a magnetic record (3.5 inch diskette) containing a listing of only the fast neutron fluence rates, their assigned elevations proper header identification of all monitor positions contained herein

ON-LINE MONITORING OF I&C TRANSMITTERS AND SENSORS FOR CALIBRATION VERIFICATION AND RESPONSE TIME TESTING WAS SUCCESSFULLY IMPLEMENTED AT ATR

Energy Technology Data Exchange (ETDEWEB)

Erickson, Phillip A.; O' Hagan, Ryan; Shumaker, Brent; Hashemian, H. M.

2017-03-01

The Advanced Test Reactor (ATR) has always had a comprehensive procedure to verify the performance of its critical transmitters and sensors, including RTDs, and pressure, level, and flow transmitters. These transmitters and sensors have been periodically tested for response time and calibration verification to ensure accuracy. With implementation of online monitoring techniques at ATR, the calibration verification and response time testing of these transmitters and sensors are verified remotely, automatically, hands off, include more portions of the system, and can be performed at almost any time during process operations. The work was done under a DOE funded SBIR project carried out by AMS. As a result, ATR is now able to save the manpower that has been spent over the years on manual calibration verification and response time testing of its temperature and pressure sensors and refocus those resources towards more equipment reliability needs. More importantly, implementation of OLM will help enhance the overall availability, safety, and efficiency. Together with equipment reliability programs of ATR, the integration of OLM will also help with I&C aging management goals of the Department of Energy and long-time operation of ATR.

Solid Acid-Catalyzed Cellulose Hydrolysis Monitored by In Situ ATR-IR Spectroscopy

NARCIS (Netherlands)

Zakzeski, J.; Grisel, R.J.H.; Smit, A.T.; Weckhuysen, B.M.

2012-01-01

The solid acid-catalyzed hydrolysis of cellulose was studied under elevated temperatures and autogenous pressures using in situ ATR-IR spectroscopy. Standards of cellulose and pure reaction products, which include glucose, fructose, hydroxymethylfurfural (HMF), levulinic acid (LA), formic acid, and

Attenuated Total Reflection Fourier Transform Infrared (ATR-FTIR) in the discrimination of normal and oral cancer blood plasma

Science.gov (United States)

Pachaiappan, Rekha; Prakasarao, Aruna; Singaravelu, Ganesan

2017-02-01

Oral cancer is the most frequent type of cancer that occurs with 75000 to 80000 new cases reported every year in India. The carcinogens from tobacco and related products are the main cause for the oral cancer. ATR-FTIR method is label free, fast and cost-effective diagnostic method would allow for rapid diagnostic results in earlier stages by the minimal chemical changes occur in the biological metabolites available in the blood plasma. The present study reports the use of ATR-FTIR data with advanced statistical model (LDA-ANN) in the diagnosis of oral cancer from normal with better accuracy. The infrared spectra were acquired on ATR-FTIR Jasco spectrophotometer at 4 cm-1 resolution, 30 scans, in the 1800-900 cm-1 spectral range. Each sample had 5 spectra recorded from each blood plasma sample. The spectral data were routed through the multilayer perception of artificial neural network to evaluate for the statistical efficacy. Among the spectral data it was found that amide II (1486 cm-1) and lipid (1526 cm-1) affords about 90 % in the discrimination between groups using LDA. These preliminary results indicate that ATR-FTIR is useful to differentiate normal subject from oral cancer patients using blood plasma.

Dual inhibition of ATR and ATM potentiates the activity of trabectedin and lurbinectedin by perturbing the DNA damage response and homologous recombination repair.

Science.gov (United States)

Lima, Michelle; Bouzid, Hana; Soares, Daniele G; Selle, Frédéric; Morel, Claire; Galmarini, Carlos M; Henriques, João A P; Larsen, Annette K; Escargueil, Alexandre E

2016-05-03

Trabectedin (Yondelis®, ecteinascidin-743, ET-743) is a marine-derived natural product approved for treatment of advanced soft tissue sarcoma and relapsed platinum-sensitive ovarian cancer. Lurbinectedin is a novel anticancer agent structurally related to trabectedin. Both ecteinascidins generate DNA double-strand breaks that are processed through homologous recombination repair (HRR), thereby rendering HRR-deficient cells particularly sensitive. We here characterize the DNA damage response (DDR) to trabectedin and lurbinectedin in HeLa cells. Our results show that both compounds activate the ATM/Chk2 (ataxia-telangiectasia mutated/checkpoint kinase 2) and ATR/Chk1 (ATM and RAD3-related/checkpoint kinase 1) pathways. Interestingly, pharmacological inhibition of Chk1/2, ATR or ATM is not accompanied by any significant improvement of the cytotoxic activity of the ecteinascidins while dual inhibition of ATM and ATR strongly potentiates it. Accordingly, concomitant inhibition of both ATR and ATM is an absolute requirement to efficiently block the formation of γ-H2AX, MDC1, BRCA1 and Rad51 foci following exposure to the ecteinascidins. These results are not restricted to HeLa cells, but are shared by cisplatin-sensitive and -resistant ovarian carcinoma cells. Together, our data identify ATR and ATM as central coordinators of the DDR to ecteinascidins and provide a mechanistic rationale for combining these compounds with ATR and ATM inhibitors.

Rabi like angular splitting in Surface Plasmon Polariton - Exciton interaction in ATR configuration

Science.gov (United States)

Hassan, Heba; Abdallah, T.; Negm, S.; Talaat, H.

2018-05-01

We have studied the coupling of propagating Surface Plasmon Polaritons (SPP) on silver films and excitons in CdS quantum dots (QDs). We employed the Kretschmann-Raether configuration of the attenuated total reflection (ATR) to propagate the SPP on silver film of thickness 47.5 nm at three different wavelengths. The CdS QD have been chemically synthesized with particular size such that its exciton of energy would resonate with SPP. High resolution transmission electron microscopy (HRTEM) and scan tunneling microscopy (STM) were used to measure the corresponding QDs size and confirm its shape. Further confirmation of the size has been performed by the effective mass approximation (EMA) model utilizing the band gap of the prepared QDs. The band gaps have been measured through UV-vis absorption spectra as well as scan tunneling spectroscopy (STS). The coupling has been observed as two branching dips in the ATR spectra indicating Rabi like splitting. To the best of our knowledge, this is the first time that Rabi interaction is directly observed in an ATR angular spectra. This observation is attributed to the use a high resolution angular scan (±0.005°), in addition to the Doppler width of the laser line as well as the energy distribution of the excitons. The effect of three different linker molecules (TOPO, HDA), (Pyridine) and (Tri-butylamine) as surface ligands, on SPP-Exciton interaction has been examined.

Investigation on the adsorption characteristics of sodium benzoate and taurine on gold nanoparticle film by ATR-FTIR spectroscopy

Science.gov (United States)

Kumar, Naveen; Thomas, S.; Tokas, R. B.; Kshirsagar, R. J.

2014-01-01

Fourier transform infrared (FTIR) spectroscopic studies of sodium benzoate and taurine adsorbed on gold nanoparticle (AuNp) film on silanised glass slides have been studied by attenuated total reflection technique (ATR). The surface morphology of the AuNp films has been measured by Atomic Force Microscopy. The ATR spectra of sodium benzoate and taurine deposited on AuNp film are compared with ATR spectra of their powdered bulk samples. A new red-shifted band appeared along with the symmetric and asymmetric stretches of carboxylate group of sodium benzoate leading to a broadening of the above peaks. Similar behavior is also seen in the case of symmetric and asymmetric stretches of sulphonate group of taurine. The results indicate presence of both chemisorbed and physisorbed layers of both sodium benzoate and taurine on the AuNp film with bottom layer chemically bound to AuNp through carboxylate and sulphonate groups respectively.

Neutronics analysis of the DHCE experiment in ATR-ITV

Energy Technology Data Exchange (ETDEWEB)

Gomes, I.C.; Smith, D.L.; Tsai, H. [Argonne National Lab., IL (United States)

1997-08-01

The preliminary analysis of the DHCE experiments in the ITV and ATR was performed and its was concluded that such a vehicle is suitable for this kind of experiment. It is recommended to place an extra filter material in the thermocouple sleeve (such as B-10), to improve the helium to dpa ratio profile during irradiation. Also, it was concluded that a preliminary estimation of period of time for replacement of the external filter would be around 5 dps`s.

Context and Quasi-Invariants in Automatic Target Recognition (ATR) with Synthetic Aperture Radar (SAR) Imagery

National Research Council Canada - National Science Library

Binford, Thomas

2000-01-01

.... Experiments based on conventional recognition techniques were conducted for comparisons. Study of persistent scattering confirms the feasibility of implementing a SAR ATR system using physical image features...

Raman and IR-ATR spectroscopy studies of heteroepitaxial structures with a GaN:C top layer

Science.gov (United States)

Cerqueira, M. F.; Vieira, L. G.; Alves, A.; Correia, R.; Huber, M.; Andreev, A.; Bonanni, A.; Vasilevskiy, M. I.

2017-09-01

This work, motivated by the technologically important task of determination of carbon dopant location in the GaN crystal lattice, employed Raman spectroscopy, with both resonant and non-resonant excitation, and infrared (IR) spectroscopy, in the attenuated total reflection (ATR) configuration, to study lattice vibration modes in a set of carbon-doped GaN (GaN:C) epilayers grown by metalorganic vapour phase epitaxy. We analyse Raman and IR-ATR spectra from the point of view of possible effects of the carbon doping, namely: (i) local vibration mode of C atoms in the nitrogen sublattice (whose frequency we theoretically estimate as 768 cm-1 using an isotope defect model), and (ii) shift in the positions of longitudinal modes owing to the phonon-plasmon coupling. We find only indirect hints of the doping effect on the resonant Raman spectra. However, we show theoretically and confirm experimentally that the IR-ATR spectroscopy can be a much more sensitive tool for this purpose, at least for the considered structures. A weak perturbation of the dielectric function of GaN:C, caused by the substitutional carbon impurity, is shown to produce a measurable dip in the ATR reflectivity spectra at  ≈770 cm-1 for both p- and s-polarizations. Moreover, it influences a specific (guided-wave type) mode observed at  ≈737 cm-1, originating from the GaN layer, which appears in the narrow frequency window where the real parts of the two components of the dielectric tensor of the hexagonal crystal have opposite signs. This interpretation is supported by our modelling of the whole multilayer structure, using a transfer matrix formalism.

ATR Mediates a Checkpoint at the Nuclear Envelope in Response to Mechanical Stress

Czech Academy of Sciences Publication Activity Database

Kumar, A.; Mazzanti, M.; Mistrik, M.; Košař, Martin; Beznoussenko, G.V.; Mironov, A. A.; Garrè, M.; Parazolli, D.; Shivashankar, G. V.; Scita, G.; Bartek, Jiří; Foiani, M.

2014-01-01

Roč. 158, č. 3 (2014), s. 633-646 ISSN 0092-8674 Grant - others:Marie Curie Intra-European(IT) 274093 Institutional support: RVO:68378050 Keywords : ATR * Mechanical Stress * cell cycle Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 32.242, year: 2014

Improving precursor adsorption characteristics in ATR-FTIR spectroscopy with a ZrO{sub 2} nanoparticle coating

Energy Technology Data Exchange (ETDEWEB)

Park, Jaeseo [Korea Research Institute of Standards and Science, Center for Vacuum Technology (Korea, Republic of); Mun, Jihun [University of Science and Technology, Department of Advanced Device Technology (Korea, Republic of); Shin, Jae-Soo; Kim, Jongho; Park, Hee Jung [Daejeon University, Department of Advanced Materials Engineering (Korea, Republic of); Kang, Sang-Woo, E-mail: swkang@kriss.re.kr [Korea Research Institute of Standards and Science, Center for Vacuum Technology (Korea, Republic of)

2017-02-15

Nanoparticles were applied to a crystal surface to increase its precursor adsorption efficiency in an attenuated total reflection Fourier transform infrared (ATR-FTIR) spectrometer. Nanoparticles with varying dispersion stabilities were employed and the resulting precursor adsorption characteristics were assessed. The size of the nanoparticles was <100 nm (TEM). In order to vary the dispersion stability, ZrO{sub 2} nanoparticles were dispersed in aqueous solutions of different pH. The ZrO{sub 2} dispersion solutions were analyzed using scanning electron microscopy (SEM) while particle distribution measurements were analyzed using electrophoretic light scattering (ELS) and dynamic light scattering (DLS) techniques. ZrO{sub 2} nanoparticles dispersed in solutions of pH 3 and 11 exhibited the most stable zeta potentials (≥+30 or ≤−30 mV); these observations were confirmed by SEM analysis and particle distribution measurements. Hexamethyldisilazane (HMDS) was used as a precursor for ATR-FTIR spectroscopy. Consequently, when ZrO{sub 2} nanoparticle solutions with the best dispersion stabilities (pH 3 and 11) were applied to the adsorption crystal surface, the measurement efficiency of ATR-FTIR spectroscopy improved by ∼200 and 300%, respectively.

The orally active and bioavailable ATR kinase inhibitor AZD6738 potentiates the anti-tumor effects of cisplatin to resolve ATM-deficient non-small cell lung cancer in vivo.

Science.gov (United States)

Vendetti, Frank P; Lau, Alan; Schamus, Sandra; Conrads, Thomas P; O'Connor, Mark J; Bakkenist, Christopher J

2015-12-29

ATR and ATM are DNA damage signaling kinases that phosphorylate several thousand substrates. ATR kinase activity is increased at damaged replication forks and resected DNA double-strand breaks (DSBs). ATM kinase activity is increased at DSBs. ATM has been widely studied since ataxia telangiectasia individuals who express no ATM protein are the most radiosensitive patients identified. Since ATM is not an essential protein, it is widely believed that ATM kinase inhibitors will be well-tolerated in the clinic. ATR has been widely studied, but advances have been complicated by the finding that ATR is an essential protein and it is widely believed that ATR kinase inhibitors will be toxic in the clinic. We describe AZD6738, an orally active and bioavailable ATR kinase inhibitor. AZD6738 induces cell death and senescence in non-small cell lung cancer (NSCLC) cell lines. AZD6738 potentiates the cytotoxicity of cisplatin and gemcitabine in NSCLC cell lines with intact ATM kinase signaling, and potently synergizes with cisplatin in ATM-deficient NSCLC cells. In contrast to expectations, daily administration of AZD6738 and ATR kinase inhibition for 14 consecutive days is tolerated in mice and enhances the therapeutic efficacy of cisplatin in xenograft models. Remarkably, the combination of cisplatin and AZD6738 resolves ATM-deficient lung cancer xenografts.

DNA-PK, ATM and ATR collaboratively regulate p53-RPA interaction to facilitate homologous recombination DNA repair.

Science.gov (United States)

Serrano, M A; Li, Z; Dangeti, M; Musich, P R; Patrick, S; Roginskaya, M; Cartwright, B; Zou, Y

2013-05-09

Homologous recombination (HR) and nonhomologous end joining (NHEJ) are two distinct DNA double-stranded break (DSB) repair pathways. Here, we report that DNA-dependent protein kinase (DNA-PK), the core component of NHEJ, partnering with DNA-damage checkpoint kinases ataxia telangiectasia mutated (ATM) and ATM- and Rad3-related (ATR), regulates HR repair of DSBs. The regulation was accomplished through modulation of the p53 and replication protein A (RPA) interaction. We show that upon DNA damage, p53 and RPA were freed from a p53-RPA complex by simultaneous phosphorylations of RPA at the N-terminus of RPA32 subunit by DNA-PK and of p53 at Ser37 and Ser46 in a Chk1/Chk2-independent manner by ATR and ATM, respectively. Neither the phosphorylation of RPA nor of p53 alone could dissociate p53 and RPA. Furthermore, disruption of the release significantly compromised HR repair of DSBs. Our results reveal a mechanism for the crosstalk between HR repair and NHEJ through the co-regulation of p53-RPA interaction by DNA-PK, ATM and ATR.

ATR-FTIR for rapid detection and quantification of counterfeit medicines

OpenAIRE

Ogwu, John; Lawson, Graham; Tanna, Sangeeta

2015-01-01

From therapeutic to lifestyle medicines, the counterfeiting of medicines has been on the rise in recent times [1]. Estimates indicate that about 10% of medicines worldwide are counterfeits with much higher figures in developing countries [2]. Currently, the rapid screening of medicines is a challenge leaving many patients at risk [1]. This study considered the potential use of Attenuated Total Reflectance-Fourier Transform Infrared Spectroscopy (ATR-FTIR) for rapid quantitative analysis of ta...

Direct Binding to Replication Protein A (RPA)-coated Single-stranded DNA Allows Recruitment of the ATR Activator TopBP1 to Sites of DNA Damage*

Science.gov (United States)

Acevedo, Julyana; Yan, Shan; Michael, W. Matthew

2016-01-01

A critical event for the ability of cells to tolerate DNA damage and replication stress is activation of the ATR kinase. ATR activation is dependent on the BRCT (BRCA1 C terminus) repeat-containing protein TopBP1. Previous work has shown that recruitment of TopBP1 to sites of DNA damage and stalled replication forks is necessary for downstream events in ATR activation; however, the mechanism for this recruitment was not known. Here, we use protein binding assays and functional studies in Xenopus egg extracts to show that TopBP1 makes a direct interaction, via its BRCT2 domain, with RPA-coated single-stranded DNA. We identify a point mutant that abrogates this interaction and show that this mutant fails to accumulate at sites of DNA damage and that the mutant cannot activate ATR. These data thus supply a mechanism for how the critical ATR activator, TopBP1, senses DNA damage and stalled replication forks to initiate assembly of checkpoint signaling complexes. PMID:27129245

AGR-2 Final Data Qualification Report for U.S. Capsules - ATR Cycles 147A Through 154B

Energy Technology Data Exchange (ETDEWEB)

Pham, Binh T. [Idaho National Lab. (INL), Idaho Falls, ID (United States). Very High-Temperature Reactor Technology Development Office; Einerson, Jeffrey J. [Idaho National Lab. (INL), Idaho Falls, ID (United States). Very High-Temperature Reactor Technology Development Office

2014-07-01

This report provides the data qualification status of AGR-2 fuel irradiation experimental data in four U.S. capsules from all 15 Advanced Test Reactor (ATR) Cycles 147A, 148A, 148B, 149A, 149B, 150A, 150B, 151A, 151B, 152A, 152B, 153A, 153B, 154A, and 154B, as recorded in the Nuclear Data Management and Analysis System (NDMAS). Thus, this report covers data qualification status for the entire AGR-2 irradiation and will replace four previously issued AGR-2 data qualification reports (e.g., INL/EXT-11-22798, INL/EXT-12-26184, INL/EXT-13-29701, and INL/EXT-13-30750). During AGR-2 irradiation, two cycles, 152A and 153A, occurred when the ATR core was briefly at low power, so AGR-2 irradiation data are not used for physics and thermal calculations. Also, two cycles, 150A and 153B, are Power Axial Locator Mechanism (PALM) cycles when the ATR power is higher than during normal cycles. During the first PALM cycle, 150A, the experiment was temporarily moved from the B-12 location to the ATR water canal and during the second PALM cycle, 153B, the experiment was temporarily moved from the B-12 location to the I-24 location to avoid being overheated. During the “Outage” cycle, 153A, seven flow meters were installed downstream from seven Fission Product Monitoring System (FPMS) monitors to measure flows from the monitors and these data are included in the NDMAS database.

Attenuated Total Reflection Fourier Transform Infrared (ATR FT-IR) for Rapid Determination of Microbial Cell Lipid Content: Correlation with Gas Chromatography-Mass Spectrometry (GC-MS).

Science.gov (United States)

Millan-Oropeza, Aaron; Rebois, Rolando; David, Michelle; Moussa, Fathi; Dazzi, Alexandre; Bleton, Jean; Virolle, Marie-Joelle; Deniset-Besseau, Ariane

2017-10-01

There is a growing interest worldwide for the production of renewable oil without mobilizing agriculture lands; fast and reliable methods are needed to identify highly oleaginous microorganisms of potential industrial interest. The aim of this study was to demonstrate the relevance of attenuated total reflection (ATR) spectroscopy to achieve this goal. To do so, the total lipid content of lyophilized samples of five Streptomyces strains with varying lipid content was assessed with two classical quantitative but time-consuming methods, gas chromatography-mass spectrometry (GC-MS) and ATR Fourier transform infrared (ATR FT-IR) spectroscopy in transmission mode with KBr pellets and the fast ATR method, often questioned for its lack of reliability. A linear correlation between these three methods was demonstrated allowing the establishment of equations to convert ATR values expressed as CO/amide I ratio, into micrograms of lipid per milligram of biomass. The ATR method proved to be as reliable and quantitative as the classical GC-MS and FT-IR in transmission mode methods but faster and more reproducible than the latter since it involves far less manipulation for sample preparation than the two others. Attenuated total reflection could be regarded as an efficient fast screening method to identify natural or genetically modified oleaginous microorganisms by the scientific community working in the field of bio-lipids.

Centromeric DNA replication reconstitution reveals DNA loops and ATR checkpoint suppression.

Science.gov (United States)

Aze, Antoine; Sannino, Vincenzo; Soffientini, Paolo; Bachi, Angela; Costanzo, Vincenzo

2016-06-01

Half of the human genome is made up of repetitive DNA. However, mechanisms underlying replication of chromosome regions containing repetitive DNA are poorly understood. We reconstituted replication of defined human chromosome segments using bacterial artificial chromosomes in Xenopus laevis egg extract. Using this approach we characterized the chromatin assembly and replication dynamics of centromeric alpha-satellite DNA. Proteomic analysis of centromeric chromatin revealed replication-dependent enrichment of a network of DNA repair factors including the MSH2-6 complex, which was required for efficient centromeric DNA replication. However, contrary to expectations, the ATR-dependent checkpoint monitoring DNA replication fork arrest could not be activated on highly repetitive DNA due to the inability of the single-stranded DNA binding protein RPA to accumulate on chromatin. Electron microscopy of centromeric DNA and supercoil mapping revealed the presence of topoisomerase I-dependent DNA loops embedded in a protein matrix enriched for SMC2-4 proteins. This arrangement suppressed ATR signalling by preventing RPA hyper-loading, facilitating replication of centromeric DNA. These findings have important implications for our understanding of repetitive DNA metabolism and centromere organization under normal and stressful conditions.

PRP19 transforms into a sensor of RPA-ssDNA after DNA damage and drives ATR activation via a ubiquitin-mediated circuitry.

Science.gov (United States)

Maréchal, Alexandre; Li, Ju-Mei; Ji, Xiao Ye; Wu, Ching-Shyi; Yazinski, Stephanie A; Nguyen, Hai Dang; Liu, Shizhou; Jiménez, Amanda E; Jin, Jianping; Zou, Lee

2014-01-23

PRP19 is a ubiquitin ligase involved in pre-mRNA splicing and the DNA damage response (DDR). Although the role for PRP19 in splicing is well characterized, its role in the DDR remains elusive. Through a proteomic screen for proteins that interact with RPA-coated single-stranded DNA (RPA-ssDNA), we identified PRP19 as a sensor of DNA damage. PRP19 directly binds RPA and localizes to DNA damage sites via RPA, promoting RPA ubiquitylation in a DNA-damage-induced manner. PRP19 facilitates the accumulation of ATRIP, the regulatory partner of the ataxia telangiectasia mutated and Rad3-related (ATR) kinase, at DNA damage sites. Depletion of PRP19 compromised the phosphorylation of ATR substrates, recovery of stalled replication forks, and progression of replication forks on damaged DNA. Importantly, PRP19 mutants that cannot bind RPA or function as an E3 ligase failed to support the ATR response, revealing that PRP19 drives ATR activation by acting as an RPA-ssDNA-sensing ubiquitin ligase during the DDR. Copyright © 2014 Elsevier Inc. All rights reserved.

Fission yeast shelterin regulates DNA polymerases and Rad3(ATR kinase to limit telomere extension.

Directory of Open Access Journals (Sweden)

Ya-Ting Chang

2013-11-01

Full Text Available Studies in fission yeast have previously identified evolutionarily conserved shelterin and Stn1-Ten1 complexes, and established Rad3(ATR/Tel1(ATM-dependent phosphorylation of the shelterin subunit Ccq1 at Thr93 as the critical post-translational modification for telomerase recruitment to telomeres. Furthermore, shelterin subunits Poz1, Rap1 and Taz1 have been identified as negative regulators of Thr93 phosphorylation and telomerase recruitment. However, it remained unclear how telomere maintenance is dynamically regulated during the cell cycle. Thus, we investigated how loss of Poz1, Rap1 and Taz1 affects cell cycle regulation of Ccq1 Thr93 phosphorylation and telomere association of telomerase (Trt1(TERT, DNA polymerases, Replication Protein A (RPA complex, Rad3(ATR-Rad26(ATRIP checkpoint kinase complex, Tel1(ATM kinase, shelterin subunits (Tpz1, Ccq1 and Poz1 and Stn1. We further investigated how telomere shortening, caused by trt1Δ or catalytically dead Trt1-D743A, affects cell cycle-regulated telomere association of telomerase and DNA polymerases. These analyses established that fission yeast shelterin maintains telomere length homeostasis by coordinating the differential arrival of leading (Polε and lagging (Polα strand DNA polymerases at telomeres to modulate Rad3(ATR association, Ccq1 Thr93 phosphorylation and telomerase recruitment.

Inhibition of DNA-PKcs enhances radiosensitivity and increases the levels of ATM and ATR in NSCLC cells exposed to carbon ion irradiation.

Science.gov (United States)

Yang, Lina; Liu, Yuanyuan; Sun, Chao; Yang, Xinrui; Yang, Zhen; Ran, Juntao; Zhang, Qiuning; Zhang, Hong; Wang, Xinyu; Wang, Xiaohu

2015-11-01

Non-small cell lung cancer (NSCLC) exhibits radioresistance to conventional rays, due to its DNA damage repair systems. NSCLC may potentially be sensitized to radiation treatment by reducing those factors that continuously enhance the repair of damaged DNA. In the present study, normal lung fibroblast MRC-5 and lung cancer A549 cells were treated with NU7026 and CGK733, which are inhibitors of the DNA-dependent protein kinase catalytic subunit (PKcs) and ataxia telangiectasia mutated (ATM) and ataxia telangiectasia and Rad3-related (ATR), respectively, followed by exposure to X-rays and carbon ion irradiation. The cytotoxic activity, cell survival rate, DNA damage repair ability, cell cycle arrest and apoptosis rate of the treated cells were analyzed with MTT assay, colony formation assay, immunofluorescence and flow cytometry, respectively. The transcription and translation levels of the ATM, ATR and DNA-PKcs genes were detected by reverse transcription-quantitative polymerase chain reaction and western blotting, respectively. The results indicated that the radiosensitivity and DNA repair ability of A549 cells were reduced, and the percentages of apoptotic cells and those arrested at the G 2 /M phase of the cell cycle were significantly increased, following ionizing radiation with inhibitor-pretreatment. The expression levels of ATM, ATR, DNA-PKcs and phosphorylated histone H2AX, a biomarker for DNA double-strand breaks, were all upregulated at the transcriptional or translational level in A549 cells treated with carbon ion irradiation, compared with the control and X-rays-treated cells. In addition, the treatment with 5-50 µM NU7026 or CGK733 did not produce any obvious cytotoxicity in MRC-5 cells, and the effect of the DNA-PKcs-inhibitor on enhancing the radiosensitivity of A549 cells was stronger than that observed for the ATM and ATR-inhibitor. These findings demonstrated a minor role for ATM and ATR in radiation-induced cell death, since the upregulation of

Decommissioning project feedback experience in the Japan Atomic Energy Research Institut

International Nuclear Information System (INIS)

Yanagihara, S.; Tachibana, M.; Miyajima, K.

2003-01-01

Since starting the research and development program for peaceful use of nuclear energy in 1950's, various research and demonstration facilities have been constructed in research organizations, universities and commercial sectors in Japan. Some of the nuclear facilities constructed in the early stage of research and development have been retired to be decommissioned because of completion of the initial objectives in the Japan Atomic Energy Research Institute (JAERI). On the other hand, since the first commercial operation of nuclear power plant (1968), the number of nuclear power plants has increased up to 52 plants operating as of August 2003 in Japan. The shear of nuclear energy accounts approximately for 35% of electricity generation in total at present time. However, several nuclear power plants have been operated for more than 25 years and two nuclear power plants are expected to be finally shutdown by 2010 to be eventually decommissioned. The Tokai Power Station, the oldest Japanese nuclear power plant operated by the Japan Atomic Power Company, was permanently shutdown from March 1998 and it is in decommissioning stage at this time. The Fugen, which is advanced thermal reactor operated by the Japan Nuclear Cycle Development Institute (JNC), was finally shutdown on March, 2003 after 25 years operation to be decommissioned. In addition, relating to planned unification between JAERI and JNC in 2005, the studies have been in progress on decommissioning and radioactive waste treatment and disposal; the cost was estimated to be 10 to 30 billion Japanese yens per year during 80 years for decommissioning of nearly 200 nuclear facilities. Decommissioning of nuclear facilities is thus getting to be one of important issues in Japan. Decommissioning of nuclear facilities might be possible using conventional and/or partially improved technology. However, reviewing project feedback experience on decommissioning and decontamination might contribute to solve various issues

Application of FTIR-ATR Spectroscopy to Determine the Extent of Lipid Peroxidation in Plasma during Haemodialysis

Directory of Open Access Journals (Sweden)

Adam Oleszko

2015-01-01

Full Text Available During a haemodialysis (HD, because of the contact of blood with the surface of the dialyser, the immune system becomes activated and reactive oxygen species (ROS are released into plasma. Particularly exposed to the ROS are lipids and proteins contained in plasma, which undergo peroxidation. The main breakdown product of oxidized lipids is the malondialdehyde (MDA. A common method for measuring the concentration of MDA is a thiobarbituric acid reactive substances (TBARS method. Despite the formation of MDA in plasma during HD, its concentration decreases because it is removed from the blood in the dialyser. Therefore, this research proposes the Fourier Transform Infrared Attenuated Total Reflectance (FTIR-ATR spectroscopy, which enables determination of primary peroxidation products. We examined the influence of the amount of hydrogen peroxide added to lipid suspension that was earlier extracted from plasma specimen on lipid peroxidation with use of TBARS and FTIR-ATR methods. Linear correlation between these methods was shown. The proposed method was effective during the evaluation of changes in the extent of lipid peroxidation in plasma during a haemodialysis in sheep. A measurement using the FTIR-ATR showed an increase in plasma lipid peroxidation after 15 and 240 minutes of treatment, while the TBARS concentration was respectively lower.

HMGA2 Inhibits Apoptosis through Interaction with ATR-CHK1 Signaling Complex in Human Cancer Cells

Directory of Open Access Journals (Sweden)

Suchitra Natarajan

2013-03-01

Full Text Available The non-histone chromatin binding protein high mobility group AT-hook 2 (HMGA2 is expressed in stem cells and many cancer cells, including tumor initiating cells, but not translated in normal human somatic cells. The presence of HMGA2 is correlated with advanced neoplastic disease and poor prognosis for patients. We had previously demonstrated a role of HMGA2 in DNA repair pathways. In the present study, we employed different human tumor cell models with endogenous and exogenous expression of HMGA2 and show that upon DNA damage, the presence of HMGA2 caused an increased and sustained phosphorylation of the ataxia telangiectasia and Rad3-related kinase (ATR and its downstream target checkpoint kinase 1 (CHK1. The presence of activated pCHK1Ser296 coincided with prolonged G2/M block and increased tumor cell survival, which was enhanced further in the presence of HMGA2. Our study, thus, identifies a novel relationship between the ATR-CHK1 DNA damage response pathway and HMGA2, which may support the DNA repair function of HMGA2 in cancer cells. Furthermore, our data provide a rationale for the use of inhibitors to ATR or CHK1 and HMGA2 in the treatment of HMGA2-positive human cancer cells.

Assessment of the applicability of AWJ technique for dismantling the reactor of Fugen. Performance of underwater-cutting thick plate and testing of sound-based monitoring for underwater-cutting process

International Nuclear Information System (INIS)

Maruyama, Shin-ichiro; Nishio, Shin-ichi

2010-01-01

The reactor of Fugen is characterized by its double-walled pressure tube construction that is composed of pressure tubes and calandria tubes. The reactor dismantlement has been planning on dismantling it under water and the abrasive water jet (AWJ) underwater-cutting method is chosen as an option among simultaneous double tubes cutting technologies. For assessing the applicability of the AWJ cutting technology, a thick plate was cut under water by the small AWJ cutting machine. In addition, since cutting causes muddiness in water, cutting was monitored by the sound-based monitoring system which was adopted as a secondary cutting monitoring method. As a results, it was demonstrated that one-phase cutting was possible under water for a stainless-steel plate with 150mm thickness and that the relationship between cutting depth and capable cutting speed could be predictable. As for the sound-based cutting monitoring, the predictability whether or not cutting would be successful was verified by checking the change of sounds level. (author)

Validation of ATR FT-IR to identify polymers of plastic marine debris, including those ingested by marine organisms

Science.gov (United States)

Jung, Melissa R.; Horgen, F. David; Orski, Sara V.; Rodriguez, Viviana; Beers, Kathryn L.; Balazs, George H.; Jones, T. Todd; Work, Thierry M.; Brignac, Kayla C.; Royer, Sarah-Jeanne; Hyrenbach, David K.; Jensen, Brenda A.; Lynch, Jennifer M.

2018-01-01

Polymer identification of plastic marine debris can help identify its sources, degradation, and fate. We optimized and validated a fast, simple, and accessible technique, attenuated total reflectance Fourier transform infrared spectroscopy (ATR FT-IR), to identify polymers contained in plastic ingested by sea turtles. Spectra of consumer good items with known resin identification codes #1–6 and several #7 plastics were compared to standard and raw manufactured polymers. High temperature size exclusion chromatography measurements confirmed ATR FT-IR could differentiate these polymers. High-density (HDPE) and low-density polyethylene (LDPE) discrimination is challenging but a clear step-by-step guide is provided that identified 78% of ingested PE samples. The optimal cleaning methods consisted of wiping ingested pieces with water or cutting. Of 828 ingested plastics pieces from 50 Pacific sea turtles, 96% were identified by ATR FT-IR as HDPE, LDPE, unknown PE, polypropylene (PP), PE and PP mixtures, polystyrene, polyvinyl chloride, and nylon.

Roles of nibrin and ATM/ATR kinases on the G2 checkpoint under endogenous or radio-induced DNA damage

Directory of Open Access Journals (Sweden)

Katherine Marcelain

2005-01-01

Full Text Available Checkpoint response to DNA damage involves the activation of DNA repair and G2 lengthening subpathways. The roles of nibrin (NBS1 and the ATM/ATR kinases in the G2 DNA damage checkpoint, evoked by endogenous and radio-induced DNA damage, were analyzed in control, A-T and NBS lymphoblast cell lines. Short-term responses to G2 treatments were evaluated by recording changes in the yield of chromosomal aberrations in the ensuing mitosis, due to G2 checkpoint adaptation, and also in the duration of G2 itself. The role of ATM/ATR in the G2 checkpoint pathway repairing chromosomal aberrations was unveiled by caffeine inhibition of both kinases in G2. In the control cell lines, nibrin and ATM cooperated to provide optimum G2 repair for endogenous DNA damage. In the A-T cells, ATR kinase substituted successfully for ATM, even though no G2 lengthening occurred. X-ray irradiation (0.4 Gy in G2 increased chromosomal aberrations and lengthened G2, in both mutant and control cells. However, the repair of radio-induced DNA damage took place only in the controls. It was associated with nibrin-ATM interaction, and ATR did not substitute for ATM. The absence of nibrin prevented the repair of both endogenous and radio-induced DNA damage in the NBS cells and partially affected the induction of G2 lengthening.

Differential Processing of Low and High LET Radiation Induced DNA Damage: Investigation of Switch from ATM to ATR Signaling

Science.gov (United States)

Saha, Janapriya; Wang, Minli; Hada, Megumi; Cucinotta, Francis A.

2011-01-01

The members of the phosphatidylinositol kinase-like kinase family of proteins namely ataxia-telangiectasia mutated (ATM) and ATM- and Rad3-related (ATR) are directly responsible for the maintenance of genomic integrity by mounting DDR through signaling and facilitating the recruitment of repair factors at the sites of DNA damage along with coordinating the deployment of cell cycle checkpoints to permit repair by phosphorylating Checkpoint kinase Chk1, Chk2 and p53. High LET radiation from GCR (Galactic Cosmic Rays) consisting mainly of protons and high energy and charged (HZE) particles from SPE (Solar Particle Event) pose a major health risk for astronauts on their space flight missions. The determination of these risks and the design of potential safeguards require sound knowledge of the biological consequences of lesion induction and the capability of the cells to counter them. We here strive to determine the coordination of ATM and ATR kinases at the break sites directly affecting checkpoint signaling and DNA repair and whether differential processing of breaks induced by low and high LET radiation leads to possible augmentation of swap of these damage sensors at the sites of DNA damage. Exposure of cells to IR triggers rapid autophosphorylation of serine-1981 that causes dimer dissociation and initiates monomer formation of ATM. ATM kinase activity depends on the disruption of the dimer, which allows access and phosphorylation of downstream ATM substrates like Chk2. Evidence suggests that ATM is activated by the alterations in higher-order chromatin structure although direct binding of ATM to DSB ends may be a crucial step in its activation. On the other hand, in case of ATR, RPA (replication protein A)-coated ssDNA (single-stranded DNA) generated as a result of stalled DNA replication or during processing of chromosomal lesions is crucial for the localization of ATR to sites of DNA damage in association with ATR-interacting protein (ATRIP). Although the

Monitoring of biofilm growth using ATR-leaky mode spectroscopy

International Nuclear Information System (INIS)

Leitz, M.; Franke, H.; Grattan, K.T.V.; Tamachkiarow, A.

2002-01-01

An approach to the in situ monitoring of biofilm formation using the technique of ATR-leaky mode spectroscopy is given as an example for the case of Cytophaga. The biofilm growth was studied on an aluminium layer and on a bilayer of the hydrogel agarose on aluminium. This metal was chosen because of its chemical stability in aqueous systems. The spectra obtained have been recorded using a flow cell to contain the suspension and nutrients over a period of several days. In the case considered using a prism surface coated with agarose, the experiments were performed by breeding in an incubator. (author)

Structure-Based Drug Design of Novel Potent and Selective Tetrahydropyrazolo[1,5- a ]pyrazines as ATR Inhibitors

Energy Technology Data Exchange (ETDEWEB)

Barsanti, Paul A; Aversa, Robert J.; Jin, Xianming; Pan, Yue; Lu, Yipin; Elling, Robert; Jain, Rama; Knapp, Mark; Lan, Jiong; Lin, Xiaodong; Rudewicz, Patrick; Sim, Janet; Taricani, Lorena; Thomas, George; Xiao, Linda; Yue, Qin (Novartis)

2015-01-08

A saturation strategy focused on improving the selectivity and physicochemical properties of ATR inhibitor HTS hit 1 led to a novel series of highly potent and selective tetrahydropyrazolo[1,5-a]pyrazines. Use of PI3Kα mutants as ATR crystal structure surrogates was instrumental in providing cocrystal structures to guide the medicinal chemistry designs. Detailed DMPK studies involving cyanide and GSH as trapping agents during microsomal incubations, in addition to deuterium-labeled compounds as mechanistic probes uncovered the molecular basis for the observed CYP3A4 TDI in the series.

Cadmium depletion impacts on hardening neutron spectrum for advanced fuel testing in ATR

International Nuclear Information System (INIS)

Chang, Gray S.

2011-01-01

For transmuting long-lived isotopes contained in spent nuclear fuel into shorter-lived fission products effectively is in a fast neutron spectrum reactor. In the absence of a fast spectrum test reactor in the United States of America (USA), initial irradiation testing of candidate fuels can be performed in a thermal test reactor that has been modified to produce a test region with a hardened neutron spectrum. A test region is achieved with a Cadmium (Cd) filter which can harden the neutron spectrum to a spectrum similar (although still somewhat softer) to that of the liquid metal fast breeder reactor (LMFBR). A fuel test loop with a Cd-filter has been installed within the East Flux Trap (EFT) of the Advanced Test Reactor (ATR) at the Idaho National Laboratory (INL). A detailed comparison analyses between the cadmium (Cd) filter hardened neutron spectrum in the ATR and the LMFBR fast neutron spectrum have been performed using MCWO. MCWO is a set of scripting tools that are used to couple the Monte Carlo transport code MCNP with the isotope depletion and buildup code ORIGEN-2.2. The MCWO-calculated results indicate that the Cd-filter can effectively flatten the Rim-Effect and reduce the linear heat rate (LHGR) to meet the advanced fuel testing project requirements at the beginning of irradiation (BOI). However, the filtering characteristics of Cd as a strong absorber quickly depletes over time, and the Cd-filter must be replaced for every two typical operating cycles within the EFT of the ATR. The designed Cd-filter can effectively depress the LHGR in experimental fuels and harden the neutron spectrum enough to adequately flatten the Rim-Effect in the test region. (author)

RNA-processing proteins regulate Mec1/ATR activation by promoting generation of RPA-coated ssDNA.

Science.gov (United States)

Manfrini, Nicola; Trovesi, Camilla; Wery, Maxime; Martina, Marina; Cesena, Daniele; Descrimes, Marc; Morillon, Antonin; d'Adda di Fagagna, Fabrizio; Longhese, Maria Pia

2015-02-01

Eukaryotic cells respond to DNA double-strand breaks (DSBs) by activating a checkpoint that depends on the protein kinases Tel1/ATM and Mec1/ATR. Mec1/ATR is activated by RPA-coated single-stranded DNA (ssDNA), which arises upon nucleolytic degradation (resection) of the DSB. Emerging evidences indicate that RNA-processing factors play critical, yet poorly understood, roles in genomic stability. Here, we provide evidence that the Saccharomyces cerevisiae RNA decay factors Xrn1, Rrp6 and Trf4 regulate Mec1/ATR activation by promoting generation of RPA-coated ssDNA. The lack of Xrn1 inhibits ssDNA generation at the DSB by preventing the loading of the MRX complex. By contrast, DSB resection is not affected in the absence of Rrp6 or Trf4, but their lack impairs the recruitment of RPA, and therefore of Mec1, to the DSB. Rrp6 and Trf4 inactivation affects neither Rad51/Rad52 association nor DSB repair by homologous recombination (HR), suggesting that full Mec1 activation requires higher amount of RPA-coated ssDNA than HR-mediated repair. Noteworthy, deep transcriptome analyses do not identify common misregulated gene expression that could explain the observed phenotypes. Our results provide a novel link between RNA processing and genome stability. © 2014 The Authors.

Applications of diamond crystal ATR FTIR spectroscopy to the characterization of ambers.

Science.gov (United States)

Guiliano, Michel; Asia, Laurence; Onoratini, Gérard; Mille, Gilbert

2007-08-01

Diamond crystal ATR FTIR spectroscopy is a rapid technique with virtually no sample preparation which requires small sample amounts and showed potential in the study of ambers. FTIR spectra of ambers present discriminating patterns and can be used to distinguish amber from immature resins as copal, to determine local or Baltic origin of archaeological ambers and to detect most of the falsifications encountered in the amber commercialisation.

Artificial intelligence in nuclear reactor operation

International Nuclear Information System (INIS)

Da Ruan; Benitez-Read, J.S.

2005-01-01

Assessment of four real fuzzy control applications at the MIT research reactor in the US, the FUGEN heavy water reactor in Japan, the BR1 research reactor in Belgium, and a TRIGA Mark III reactor in Mexico will be examined through a SWOT analysis (strengths, weakness, opportunities, and threats). Special attention will be paid to the current cooperation between the Belgian Nuclear Research Centre (SCK·CEN) and the Mexican Nuclear Centre (ININ) on AI-based intelligent control for nuclear reactor operation under the partial support of the National Council for Science and Technology of Mexico (CONACYT). (authors)

Structural Analysis of Spiropyran Polimers using ATR Spectroscopy

Energy Technology Data Exchange (ETDEWEB)

Delgado Macui, R; Rojas Lopez, M; Gayou, V L; Orduna Diaz, A [Centro de Investigacion en BiotecnologIa Aplicada del IPN, San Juan de los Molinos km. 1.5 Tepetitla de Lardizabal, A.P. 90700, Tlaxcala (Mexico)

2006-01-01

We have used infrared spectroscopy in attenuated total reflection (ATR) mode to analyze the interactions between the polymeric base and solvent with a photochromic material (spyropiran). We used cellulose acetate as polymeric base and the spyropiran; 1,3,3 trimethyl indoline-5-nitro benzopyrane. Thin films with different weight concentrations of SP were deposited in the polymeric base. The infrared spectra show bands whose frequencies are associated to several molecular bondings. It was observed a decreasing in intensity of absorbance for C = O stretching mode of the acetate group at 1720 cm{sup -1} and for C = C stretching mode for the main chain at 823 and 982 cm{sup -1} both associated to the presence of SP in polymeric films.

Analysis code for large rupture accidents in ATR. SENHOR/FLOOD/HEATUP

International Nuclear Information System (INIS)

1997-08-01

In the evaluation of thermo-hydraulic transient change, the behavior of core reflooding and the transient change of fuel temperature in the events which are classified in large rupture accidents of reactor coolant loss, that is the safety evaluation event of the ATR, the analysis codes for thermo-hydraulic transient change at the time of large rupture SENHOR, for core reflooding characteristics FLOOD and for fuel temperature HEATUP are used, respectively. The analysis code system for loss of coolant accident comprises the analysis code for thermo-hydraulic transient change at the time of medium and small ruptures LOTRAC in addition to the above three codes. Based on the changes with time lapse of reactor thermal output and steam drum pressure obtained by the SENHOR, average reflooding rate is analyzed by the FLOOD, and the time of starting the turnaround of fuel cladding tube temperature and the heat transfer rate after the turnaround are determined. Based on these data, the detailed temperature change of fuel elements is analyzed by the HEATUP, and the highest temperature and the amount of oxidation of fuel cladding tubes are determined. The SENHOR code, the FLOOD code and the HEATUP code and various models for these codes are explained. The example of evaluation and the sensitivity analysis of the ATR plant are reported in the Appendix. (K.I.)

Japan

DEFF Research Database (Denmark)

Hansen, Annette Skovsted

2013-01-01

I 1500- og 1600-tallet dannedes tidligt moderne stater i Europa, men samtidig eksisterede der ligeså avancerede statsdannelser uden for Europa, bl.a. i Kina, Indien og Japan. I det følgende ser vi nærmere på dannelsen af den moderne stat i Japan. Hvorfor blev Japan aldrig en europæisk koloni......? Hvordan havde japanske magthavere igennem 300 år forberedt Japan og de mennesker, der boede på de japanske øer, til at kunne udvikle en nation, der skulle blive den stærkeste og rigeste i Asien i mere end 100 år? Hvem bestemte i Japan? Kejseren eller shogunen?...

Analysis of European honeybee (Apis mellifera) wings using ATR-FTIR and Raman spectroscopy: A pilot study

Czech Academy of Sciences Publication Activity Database

Machovič, Vladimír; Lapčák, L.; Havelcová, Martina; Borecká, Lenka; Novotná, M.; Novotná, M.; Javůrková, I.; Langrová, I.; Hájková, Š.; Brožová, A.; Titěra, D.

2017-01-01

Roč. 48, č. 1 (2017), s. 22-29 ISSN 1211-3174 Institutional support: RVO:67985891 Keywords : honeybee wings * ATR-FTIR * Raman spectroscopy * protein * lipid * chitin Subject RIV: CB - Analytical Chemistry, Separation OBOR OECD: Analytical chemistry

A report of the overall working group of the AEC Committee on Development of Advanced Power Reactors

International Nuclear Information System (INIS)

1979-01-01

The AEC Committee on Development of Advanced Power Reactors was set up in April, 1978, following on the previous AEC Special Committee on Development of Advanced Power Reactors, in order to study on the complementary power reactors between current LWRs and future FBRs. The subjects of study by the overall working group are the status of advanced power reactors in views of the nuclear fuel cycle, the impacts on industries, the selection of reactor types under present international circumstances, and the evaluation of advanced power reactors in their technology and economy. The following matters are described: evaluations in view of the nuclear fuel cycle, i.e. the features of the ATR of Japan and CANDU reactors of Canada; international problems concerning nuclear nonproliferation and securing of uranium; problems in the diversification of power reactor types concerning the expenditure and technology; problems of technology in the ATR of Japan, CANDU reactors of Canada and Pu utilization for LWRs; and the economy of D 2 O power reactors, i.e. the ATR of Japan and CANDU reactors of Canada. (J.P.N.)

Study of consumer fireworks post-blast residues by ATR-FTIR.

Science.gov (United States)

Martín-Alberca, Carlos; Zapata, Félix; Carrascosa, Héctor; Ortega-Ojeda, Fernando E; García-Ruiz, Carmen

2016-03-01

Specific analytical procedures are requested for the forensic analysis of pre- and post-blast consumer firework samples, which present significant challenges. Up to date, vibrational spectroscopic techniques such as Fourier transform infrared spectroscopy (FTIR) have not been tested for the analysis of post-blast residues in spite of their interesting strengths for the forensic field. Therefore, this work proposes a simple and fast procedure for the sampling and analysis of consumer firework post-blast residues by a portable FTIR instrument with an Attenuated Total Reflection (ATR) accessory. In addition, the post-blast residues spectra of several consumer fireworks were studied in order to achieve the identification of their original chemical compositions. Hence, this work analysed 22 standard reagents usually employed to make consumer fireworks, or because they are related to their combustion products. Then, 5 different consumer fireworks were exploded, and their residues were sampled with dry cotton swabs and directly analysed by ATR-FTIR. In addition, their pre-blast fuses and charges were also analysed in order to stablish a proper comparison. As a result, the identification of the original chemical compositions of the post-blast samples was obtained. Some of the compounds found were potassium chlorate, barium nitrate, potassium nitrate, potassium perchlorate or charcoal. An additional study involving chemometric tools found that the results might greatly depend on the swab head type used for the sampling, and its sampling efficiency. The proposed procedure could be used as a complementary technique for the analysis of consumer fireworks post-blast residues. Copyright © 2015 Elsevier B.V. All rights reserved.

Proteomics Reveals Global Regulation of Protein SUMOylation by ATM and ATR Kinases during Replication Stress

Directory of Open Access Journals (Sweden)

Stephanie Munk

2017-10-01

Full Text Available The mechanisms that protect eukaryotic DNA during the cumbersome task of replication depend on the precise coordination of several post-translational modification (PTM-based signaling networks. Phosphorylation is a well-known regulator of the replication stress response, and recently an essential role for SUMOs (small ubiquitin-like modifiers has also been established. Here, we investigate the global interplay between phosphorylation and SUMOylation in response to replication stress. Using SUMO and phosphoproteomic technologies, we identify thousands of regulated modification sites. We find co-regulation of central DNA damage and replication stress responders, of which the ATR-activating factor TOPBP1 is the most highly regulated. Using pharmacological inhibition of the DNA damage response kinases ATR and ATM, we find that these factors regulate global protein SUMOylation in the protein networks that protect DNA upon replication stress and fork breakage, pointing to integration between phosphorylation and SUMOylation in the cellular systems that protect DNA integrity.

Radiation Measurements Laboratory (RML) calibration and assessment of the ATR SPING-3 stack effluent monitor

International Nuclear Information System (INIS)

Koeppen, L.D.; Rogers, J.W.; Simpson, O.D.

1983-12-01

An evaluation, calibration and assessment of the Eberline SPING-3 ATR stack effluent monitor was conducted. This unit which monitors particulate, iodine and noble gas effluents was producing abnormal results following the initial installation and operational testing. The purposes of this work were to find the causes of the abnormal results and correct them if possible; check the calibrations and adjust them if necessary; and to provide a better in-depth understanding of what the unit is monitoring and how well it performs under this application. Results have shown that there were some problems associated with the unit as initially installed and tested. These problems have been identified and suggested alternatives shown, the monitor was found to be applicable to some extent under the current conditions. The calibrations have been checked and adjustments made. More operation testing and evaluation is needed to assess how well this works under a variety of ATR operating conditions. 2 references, 10 figures, 3 tables

ATR-IR study of skin components: Lipids, proteins and water. Part I: Temperature effect

Science.gov (United States)

Olsztyńska-Janus, S.; Pietruszka, A.; Kiełbowicz, Z.; Czarnecki, M. A.

2018-01-01

In this work we report the studies of the effect of temperature on skin components, such as lipids, proteins and water. Modifications of lipids structure induced by increasing temperature (from 20 to 90 °C) have been studied using ATR-IR (Attenuated Total Reflectance Infrared) spectroscopy, which is a powerful tool for characterization of the molecular structure and properties of tissues, such as skin. Due to the small depth of penetration (0.6-5.6 μm), ATR-IR spectroscopy probes only the outermost layer of the skin, i.e. the stratum corneum (SC). The assignment of main spectral features of skin components allows for the determination of phase transitions from the temperature dependencies of band intensities [e.g. νas(CH2) and νs(CH2)]. The phase transitions were determined by using two methods: the first one was based on the first derivative of the Boltzmann function and the second one employed tangent lines of sigmoidal, aforementioned dependencies. The phase transitions in lipids were correlated with modifications of the structure of water and proteins.

Measured thermal and fast neutron fluence rates for ATF-1 holders during ATR cycle 160A

International Nuclear Information System (INIS)

Walker, B. J.; Miller, D. T.

2017-01-01

This report contains the thermal (2200 m/s) and fast (E>1MeV) neutron fluence rate data for the ATF-1 holders located in core for ATR Cycle 160A which were measured by the Radiation Measurements Laboratory (RML).

Measured thermal and fast neutron fluence rates for ATF-1 holders during ATR cycle 160A

Energy Technology Data Exchange (ETDEWEB)

Walker, B. J. [Idaho National Lab. (INL), Idaho Falls, ID (United States); Miller, D. T. [Idaho National Lab. (INL), Idaho Falls, ID (United States)

2017-06-06

This report contains the thermal (2200 m/s) and fast (E>1MeV) neutron fluence rate data for the ATF-1 holders located in core for ATR Cycle 160A which were measured by the Radiation Measurements Laboratory (RML).

Sobre el iuspositivismo que hemos de dejar atrás. Una crítica iusnaturalista a "Dejemos atrás el positivismo jurídico" de Atienza y de Ruiz Manero

Directory of Open Access Journals (Sweden)

JOSÉ CHÁVEZ-FERNÁNDEZ

2011-01-01

Full Text Available A través de la confrontación de las tesis más relevantes que Atienza y Ruiz Manero proponen en su artículo "Dejemos atrás el positivismo jurídico", el autor pretende mostrar-a partir de la respuesta a las preguntas "¿qué iuspositivismo hemos de dejar atrás?", "¿por qué?" y "¿cómo?"- que existen en el iuspositivismo debilidades explicativas graves respecto de la realidad jurídica, y que estas están vinculadas tanto al escepticismo ético como a la negación de la existencia de un Derecho natural en sentido propio, es decir, de raigambre antropológico-metafísica. Por otro lado, se tratará de evidenciar que dichas debilidades explicativas le son comunes también a los intentos pragmático-constructivistas de superación del iuspositivismo por lo que, en última instancia, ninguno de dichos esquemas conceptuales puede dar cuenta cabal de una realidad como los derechos humanos que se presentan como deudas ético-jurídicas inexcusables fundamentadas en la dignidad ontológica de todos los seres humanos.

Diamond-coated ATR prism for infrared absorption spectroscopy of surface-modified diamond nanoparticles

Czech Academy of Sciences Publication Activity Database

Remeš, Zdeněk; Kozak, Halyna; Rezek, Bohuslav; Ukraintsev, Egor; Babchenko, Oleg; Kromka, Alexander; Girard, H.A.; Arnault, J.-C.; Bergonzo, P.

2013-01-01

Roč. 270, APR (2013), s. 411-417 ISSN 0169-4332 R&D Projects: GA ČR GAP108/12/0910; GA ČR GPP205/12/P331; GA MŠk LH12236; GA MŠk LH12186 Institutional support: RVO:68378271 Keywords : ATR FTIR * CVD * hydrogenation * microwave * nanocrystalline diamond * nanopowder Subject RIV: BM - Solid Matter Physics ; Magnetism Impact factor: 2.538, year: 2013

Preliminary Assessment of ATR-C Capabilities to Provide Integral Benchmark Data for Key Structural/Matrix Materials that May be Used for Nuclear Data Testing and Analytical Methods Validation

Energy Technology Data Exchange (ETDEWEB)

John D. Bess

2009-07-01

The purpose of this document is to identify some suggested types of experiments that can be performed in the Advanced Test Reactor Critical (ATR-C) facility. A fundamental computational investigation is provided to demonstrate possible integration of experimental activities in the ATR-C with the development of benchmark experiments. Criticality benchmarks performed in the ATR-C could provide integral data for key matrix and structural materials used in nuclear systems. Results would then be utilized in the improvement of nuclear data libraries and as a means for analytical methods validation. It is proposed that experiments consisting of well-characterized quantities of materials be placed in the Northwest flux trap position of the ATR-C. The reactivity worth of the material could be determined and computationally analyzed through comprehensive benchmark activities including uncertainty analyses. Experiments were modeled in the available benchmark model of the ATR using MCNP5 with the ENDF/B-VII.0 cross section library. A single bar (9.5 cm long, 0.5 cm wide, and 121.92 cm high) of each material could provide sufficient reactivity difference in the core geometry for computational modeling and analysis. However, to provide increased opportunity for the validation of computational models, additional bars of material placed in the flux trap would increase the effective reactivity up to a limit of 1$ insertion. For simplicity in assembly manufacture, approximately four bars of material could provide a means for additional experimental benchmark configurations, except in the case of strong neutron absorbers and many materials providing positive reactivity. Future tasks include the cost analysis and development of the experimental assemblies, including means for the characterization of the neutron flux and spectral indices. Oscillation techniques may also serve to provide additional means for experimentation and validation of computational methods and acquisition of

Application of FTIR-ATR Spectroscopy for the quality control of wine spirits, brandies and grape marc spirits

OpenAIRE

Anjos, O.; Caldeira, I.

2013-01-01

This study attempted to apply a rapid method for the determination of methanol, acetaldehyde and ethyl acetate in wine spirits, brandies and grape marc spirits, using Fourier Transform Infrared – Attenuated Total Reflectance (ATR) Spectroscopy.

Gene therapy: a lipofection approach for gene transfer into primary endothelial cells.

Science.gov (United States)

Young, A T L; Lakey, J R T; Murray, A G; Moore, R B

2002-01-01

Despite the great potential of gene therapy to become a new treatment modality in future medicine, there are still many limitations to overcome before this gene approach can pass to the stage of human trial. The foremost obstacle is the development of a safe, efficient, and efficacious vector system for in vivo gene application. This study evaluated the efficacy of lipofection as a gene delivery vehicle into primary endothelial cells. Transfection efficiency of several lipid-based reagents (Effectene, Fugene 6, DOTAP) was examined at experimental temperatures of 37 degrees C, 24 degrees C, and 6 degrees C. Human umbilical vein endothelial cells (HUVECs) were transfected with the enhanced green fluorescent protein (EGFP) using precise amounts of DNA (Effectene, 0.2 microg; Fugene 6, 0.5 microg; DOTAP, 2.5 microg) and lipids (Effectene, 10 microl; Fugene 6, 6 microl; DOTAP, 15 microl) optimized in our laboratory. Duration of incubation in the DNA/lipid transfection mixture varied for each lipid transfectant as follows: 5 h for both Fugene 6 and DOTAP and 3 h for Effectene. Efficiency of transfection was quantified by microscopic evaluation of EFGP expression in a minimum of 100 cells per group. Transfection efficiencies achieved with these lipofection agents were 34 +/- 1.3% (mean +/- SEM), 33 +/- 1.4%, and 18 +/- 1.5% for Effectene, Fugene 6, and DOTAP, respectively, at 37 degrees C. Transfection results were lower at 24 degrees C with mean efficiencies of 26 +/- 2.4% for Effectene, 14 +/- 2.9% for Fugene 6, and 15 +/- 3.2% for DOTAP. Furthermore, mean efficiencies at 6 degrees C were 6 +/- 0.5%, 8 +/- 1.5%, and 6 +/- 0.0% for Effectene, Fugene 6, and DOTAP, respectively. Efficiency of transfection appeared to be temperature dependent (ANOVA; p lipofection a potential gene delivery strategy for in vivo gene therapy.

Radar automatic target recognition (ATR) and non-cooperative target recognition (NCTR)

CERN Document Server

Blacknell, David

2013-01-01

The ability to detect and locate targets by day or night, over wide areas, regardless of weather conditions has long made radar a key sensor in many military and civil applications. However, the ability to automatically and reliably distinguish different targets represents a difficult challenge. Radar Automatic Target Recognition (ATR) and Non-Cooperative Target Recognition (NCTR) captures material presented in the NATO SET-172 lecture series to provide an overview of the state-of-the-art and continuing challenges of radar target recognition. Topics covered include the problem as applied to th

Preliminary Assessment of ATR-C Capabilities to Provide Integral Benchmark Data for Key Structural/Matrix Materials that May be Used for Nuclear Data Testing and Analytical Methods Validation

Energy Technology Data Exchange (ETDEWEB)

John D. Bess

2009-03-01

The purpose of this research is to provide a fundamental computational investigation into the possible integration of experimental activities with the Advanced Test Reactor Critical (ATR-C) facility with the development of benchmark experiments. Criticality benchmarks performed in the ATR-C could provide integral data for key matrix and structural materials used in nuclear systems. Results would then be utilized in the improvement of nuclear data libraries and as a means for analytical methods validation. It is proposed that experiments consisting of well-characterized quantities of materials be placed in the Northwest flux trap position of the ATR-C. The reactivity worth of the material could be determined and computationally analyzed through comprehensive benchmark activities including uncertainty analyses. Experiments were modeled in the available benchmark model of the ATR using MCNP5 with the ENDF/B-VII.0 cross section library. A single bar (9.5 cm long, 0.5 cm wide, and 121.92 cm high) of each material could provide sufficient reactivity difference in the core geometry for computational modeling and analysis. However, to provide increased opportunity for the validation of computational models, additional bars of material placed in the flux trap would increase the effective reactivity up to a limit of 1$ insertion. For simplicity in assembly manufacture, approximately four bars of material could provide a means for additional experimental benchmark configurations, except in the case of strong neutron absorbers and many materials providing positive reactivity. Future tasks include the cost analysis and development of the experimental assemblies, including means for the characterization of the neutron flux and spectral indices. Oscillation techniques may also serve to provide additional means for experimentation and validation of computational methods and acquisition of integral data for improving neutron cross sections. Further assessment of oscillation

Increased Rrm2 gene dosage reduces fragile site breakage and prolongs survival of ATR mutant mice

DEFF Research Database (Denmark)

Lopez-Contreras, Andres J; Specks, Julia; Barlow, Jacqueline H

2015-01-01

In Saccharomyces cerevisiae, absence of the checkpoint kinase Mec1 (ATR) is viable upon mutations that increase the activity of the ribonucleotide reductase (RNR) complex. Whether this pathway is conserved in mammals remains unknown. Here we show that cells from mice carrying extra alleles of the...

Analytical characterization of polymers used in conservation and restoration by ATR-FTIR spectroscopy.

Science.gov (United States)

Chércoles Asensio, Ruth; San Andrés Moya, Margarita; de la Roja, José Manuel; Gómez, Marisa

2009-12-01

In the last few decades many new polymers have been synthesized that are now being used in cultural heritage conservation. The physical and chemical properties and the long-term behaviors of these new polymers are determined by the chemical composition of the starting materials used in their synthesis along with the nature of the substances added to facilitate their production. The practical applications of these polymers depend on their composition and form (foam, film, sheets, pressure-sensitive adhesives, heat-seal adhesives, etc.). Some materials are used in restoration works and others for the exhibition, storage and transport of works of art. In all cases, it is absolutely necessary to know their compositions. Furthermore, many different materials that are manufactured for other objectives are also used for conservation and restoration. The technical information about the materials provided by the manufacturer is usually incomplete, so it is necessary to analytically characterize such materials. FTIR spectrometry is widely used for polymer identification, and, more recently, ATR-FTIR has been shown to give excellent results. This paper reports the ATR-FTIR analysis of samples of polymeric materials used in the conservation of artworks. These samples were examined directly in the solid material without sample preparation.

Prediction of the oversulphated chondroitin sulphate contamination of unfractionated heparin by ATR-IR spectrophotometry.

Science.gov (United States)

Norwig, J; Beyer, T; Brinz, D; Holzgrabe, U; Diller, M; Manns, D

2009-03-01

The detection of a contamination of heparin with oversulphated chondroitin sulphate (OSCS) was first analysed in an unfractionated heparin batch supplied to the US API-market in April 2006. OSCS is a semi-synthetic derivative of the natural occuring glycosaminoglycan chondroitin sulphate. Moreover some spectroscopic characteristics of the substance overlap with those of heparin, so that the infrared (IR) spectra are visually difficult to distinguish whereas (1)H-NMR (Nuclear Magnetic Resonance) spectroscopy or capillary electrophoresis (CE) provides identification by a simple visual inspection of either the spectrum or the electropherogram respectively. However, applying special tools of Multivariate Data Analysis (MVA) to the IR spectra an identification of the contaminated samples is possible. In detail a rapid Attenuation Total Reflectance-Infrared (ATR-IR) measurement was selected, which does not require any sample preparation. The result (contaminated or not contaminated) is predicted within a few minutes. A method transfer to mobile ATR-IR spectrometers seems to be possible. The analysis is based on the fact that the fingerprint of the OSCS IR spectrum (1st derivative) complies with a theoretically calculated principal component in the MVA.

Characterization of Printing Inks Using DART-Q-TOF-MS and Attenuated Total Reflectance (ATR) FTIR.

Science.gov (United States)

Williamson, Rhett; Raeva, Anna; Almirall, Jose R

2016-05-01

The rise in improved and widely accessible printing technology has resulted in an interest to develop rapid and minimally destructive chemical analytical techniques that can characterize printing inks for forensic document analysis. Chemical characterization of printing inks allows for both discrimination of inks originating from different sources and the association of inks originating from the same source. Direct analysis in real-time mass spectrometry (DART-MS) and attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR) were used in tandem to analyze four different classes of printing inks: inkjets, toners, offset, and intaglio. A total of 319 samples or ~ 80 samples from each class were analyzed directly on a paper substrate using the two methods. DART-MS was found to characterize the semi-volatile polymeric vehicle components, while ATR-FTIR provided chemical information associated with the bulk components of these inks. Complimentary data results in improved discrimination when both techniques are used in succession resulting in >96% discrimination for all toners, 95% for all inkjets, >92% for all offset, and >54% for all intaglio inks. © 2016 American Academy of Forensic Sciences.

Determination of alkaloids in capsules, milk and ethanolic extracts of poppy (Papaver somniferum L.) by ATR-FT-IR and FT-Raman spectroscopy.

Science.gov (United States)

Schulz, Hartwig; Baranska, Malgorzata; Quilitzsch, Rolf; Schütze, Wolfgang

2004-10-01

Fourier transform (FT) infrared spectroscopy using a diamond composite ATR crystal and NIR-FT-Raman spectroscopy techniques were applied for the simultaneous identification and quantification of the most important alkaloids in poppy capsules. Most of the characteristic Raman signals of the alkaloids can be identified in poppy milk isolated from unripe capsules. But also poppy extracts present specific bands relating clearly to the alkaloid fraction. Raman spectra obtained by excitation with a Nd:YAG laser at 1064 nm show no disturbing fluorescence effects; therefore the plant tissue can be recorded without any special preparation. The used diamond ATR technique allows to measure very small sample amounts (5-10 microL or 2-5 mg) without the necessity to perform time-consuming pre-treatments. When applying cluster analysis a reliable discrimination of "low-alkaloid" and "high-alkaloid" poppy single-plants can be easily achieved. The examples presented in this study provide clear evidence of the benefits of Raman and ATR-IR spectroscopy in efficient quality control, forensic analysis and high-throughput evaluation of poppy breeding material.

Electrooxidation of ethanol on Pt and PtRu surfaces investigated by ATR surface-enhanced infrared absorption spectroscopy

Energy Technology Data Exchange (ETDEWEB)

Silva, Marcio F.; Camara, Giuseppe A., E-mail: giuseppe.silva@ufms.br [Departamento de Quimica, Universidade Federal do Mato Grosso do Sul, Campo Grande-MS (Brazil); Batista, Bruno C.; Boscheto, Emerson [Instituto de Quimica de Sao Carlos, Universidade de Sao Paulo, Sao Carlos-SP, (Brazil); Varela, Hamilton, E-mail: varela@iqsc.usp.br [Ertl Center for Electrochemistry and Catalysis, Gwangju Institute of Science and Technology (GIST), Gwangju (Korea, Republic of)

2012-05-15

Herein, it was investigated for the first time the electro-oxidation of ethanol on Pt and PtRu electrodeposits in acidic media by using in situ surface enhanced infrared absorption spectroscopy with attenuated total reflection (ATR-SEIRAS). The experimental setup circumvents the weak absorbance signals related to adsorbed species, usually observed for rough, electrodeposited surfaces, and allows a full description of the CO coverage with the potential for both catalysts. The dynamics of adsorption-oxidation of CO was accessed by ATR-SEIRAS experiments (involving four ethanol concentrations) and correlated with expressions derived from a simple kinetic model. Kinetic analysis suggests that the growing of the CO adsorbed layer is nor influenced by the presence of Ru neither by the concentration of ethanol. The results suggest that the C-C scission is not related to the presence of Ru and probably happens at Pt sites. (author)

Characterization and sintering of ATR aluminia from niobium

International Nuclear Information System (INIS)

Shibuya, N.H.

1987-01-01

The characterization of resultante slag from Aluminothermic Reduction (ATR) process to obtain metallic niobium is presented. The slag was characterized for concentration and phases of aluminia by X-ray diffractometry. The results show that 70% of the slag is constituted by α aluminia. The lixiviation and calcination of the slag increased the α aluminia concentration to 95%, the slag was used for producing samples to be burning in three furnaces: electrical resistance furnace in the air, and two furnaces in the vacuum. The burned samples were characterized by microscopy, ultrasonic analysis, density measurements and X-ray diffractometry. The sintering in the vacuum is possible because the samples burned in vacuum presented major density. The formation of NbO 2 and mullite was observed, by X-ray diffractometry. The data from optical microscopy, density measurements and X-ray diffractometry show high porosity. (M.C.K.) [pt

Measured thermal and fast neutron fluence rates ATR Cycle 99-A, November 23, 1992--January 23, 1993

International Nuclear Information System (INIS)

Murray, R.K.; Rogers, J.W.

1993-03-01

This report contains the thermal (2200 m/s) and fast (E>me) neutron fluence rate data for ATR Cycle 99-A which were measured by the Radiation Measurements Laboratory (RML) as requested by the Power ReactorPrograms (ATR Experiments) Radiation Measurements Work Order. This report contains fluence rate values corresponding to the particular elevations (relative to the 80 ft. core elevation) where the measurements were taken. The data in this report consists of (1) a table of the ATR power history and distribution, (2) a hard copy listing of all thermal and fast neutron fluence rates, (3) plots of both the thermal and fast neutron fluence rates, and (4) a magnetic record (3.5 inch diskette) containing a listing of only the fast neutron fluence rates, their assigned elevations and proper header identification of all monitor positions contained herein. The fluence rates reported are for the average power levels given in the table of power history and distribution. All ''H'' holder monitor wires for this cycle are 54 inches long. All ''SR'' holder monitor wires for this cycle are 55 inches long. This length allows measurement of the full core region and makes the first count elevation 24.73 inches above core midplane. Due to the safety rod problems in the west lobe, ''BR'' holders were used in the W-1, 2, 3, and 4 positions. All ''BR'' holder monitor wires for this cycle are 56.25 inches long. The distance from the end of the wires to the first count position was 4.25 inches for all wires counted from this cycle

Pharmacologic ATM but not ATR kinase inhibition abrogates p21-dependent G1 arrest and promotes gastrointestinal syndrome after total body irradiation.

Science.gov (United States)

Vendetti, Frank P; Leibowitz, Brian J; Barnes, Jennifer; Schamus, Sandy; Kiesel, Brian F; Abberbock, Shira; Conrads, Thomas; Clump, David Andy; Cadogan, Elaine; O'Connor, Mark J; Yu, Jian; Beumer, Jan H; Bakkenist, Christopher J

2017-02-01

We show that ATM kinase inhibition using AZ31 prior to 9 or 9.25 Gy total body irradiation (TBI) reduced median time to moribund in mice to 8 days. ATR kinase inhibition using AZD6738 prior to TBI did not reduce median time to moribund. The striking finding associated with ATM inhibition prior to TBI was increased crypt loss within the intestine epithelium. ATM inhibition reduced upregulation of p21, an inhibitor of cyclin-dependent kinases, and blocked G1 arrest after TBI thereby increasing the number of S phase cells in crypts in wild-type but not Cdkn1a(p21 CIP/WAF1 )-/- mice. In contrast, ATR inhibition increased upregulation of p21 after TBI. Thus, ATM activity is essential for p21-dependent arrest while ATR inhibition may potentiate arrest in crypt cells after TBI. Nevertheless, ATM inhibition reduced median time to moribund in Cdkn1a(p21 CIP/WAF1 )-/- mice after TBI. ATM inhibition also increased cell death in crypts at 4 h in Cdkn1a(p21 CIP/WAF1 )-/-, earlier than at 24 h in wild-type mice after TBI. In contrast, ATR inhibition decreased cell death in crypts in Cdkn1a(p21 CIP/WAF1 )-/- mice at 4 h after TBI. We conclude that ATM activity is essential for p21-dependent and p21-independent mechanisms that radioprotect intestinal crypts and that ATM inhibition promotes GI syndrome after TBI.

The ATM and ATR inhibitors CGK733 and caffeine suppress cyclin D1 levels and inhibit cell proliferation

International Nuclear Information System (INIS)

Alao, John P; Sunnerhagen, Per

2009-01-01

The ataxia telangiectasia mutated (ATM) and the ATM- related (ATR) kinases play a central role in facilitating the resistance of cancer cells to genotoxic treatment regimens. The components of the ATM and ATR regulated signaling pathways thus provide attractive pharmacological targets, since their inhibition enhances cellular sensitivity to chemo- and radiotherapy. Caffeine as well as more specific inhibitors of ATM (KU55933) or ATM and ATR (CGK733) have recently been shown to induce cell death in drug-induced senescent tumor cells. Addition of these agents to cancer cells previously rendered senescent by exposure to genotoxins suppressed the ATM mediated p21 expression required for the survival of these cells. The precise molecular pharmacology of these agents however, is not well characterized. Herein, we report that caffeine, CGK733, and to a lesser extent KU55933, inhibit the proliferation of otherwise untreated human cancer and non-transformed mouse fibroblast cell lines. Exposure of human cancer cell lines to caffeine and CGK733 was associated with a rapid decline in cyclin D1 protein levels and a reduction in the levels of both phosphorylated and total retinoblastoma protein (RB). Our studies suggest that observations based on the effects of these compounds on cell proliferation and survival must be interpreted with caution. The differential effects of caffeine/CGK733 and KU55933 on cyclin D1 protein levels suggest that these agents will exhibit dissimilar molecular pharmacological profiles

ATR-FTIR Spectroscopy Highlights the Problem of Distinguishing Between Exophiala dermatitidis and E. phaeomuriformis Using MALDI-TOF MS

NARCIS (Netherlands)

Ergin, C.; Gok, Y.; Baygu, Y.; Gumral, R.; Ozhak-Baysan, B.; Dogen, A.; Ogunc, D.; Ilkit, M.; Seyedmousavi, S.

2016-01-01

The present study compared two chemical-based methods, namely, matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF MS) and attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy, to understand the misidentification of Exophiala

Atròfia muscular espinal: mecanismes patogènics i estratègies terapèutiques en models murins de la malaltia

OpenAIRE

Cerveró Cebrià, Clàudia

2016-01-01

L’atròfia muscular espinal (AME) és una malaltia genètica que cursa amb mort de motoneurones espinals i atròfia muscular. S’ha caracteritzat un model murí d’AME, l’Smn2B/-, amb una clínica menys severa que la mostrada per altres models més extensament utilitzats. S’ha evidenciat una alteració multisistèmica acompanyant a la clàssicament coneguda del sistema neuromuscular. S’han estudiat les sinapsis colinèrgiques tipus C en l’AME i testat el paper del PRE-084 (agonista del receptor sigm...

ATR-IR spectroscopic cell for in situ studies at solid-liquid interface at elevated temperatures and pressures

NARCIS (Netherlands)

Koichumanova, Kamila; Visan, Aura; Geerdink, Bert; Lammertink, Rob G.H.; Mojet, Barbara; Seshan, Kulathuiyer; Lefferts, Leonardus

2017-01-01

An in situ ATR-IR spectroscopic cell suitable for studies at solid-liquid interface is described including the design and experimental details in continuous flow mode at elevated temperatures (230 °C) and pressures (30 bar). The design parameters considered include the cell geometry, the procedure

Competition in Japan

OpenAIRE

Michael E. Porter; Mariko Sakakibara

2004-01-01

This article examines competition in Japan and its link to postwar economic prosperity. While Japan's industrial structure and competition policy seem to indicate that competition in Japan has been less intense, the empirical evidence does not support this conclusion. The sectors in which competition was restricted prove to be those where Japan was not internationally successful. In the internationally successful sectors, internal competition in Japan was invariably fierce. While the level of...

In-column ATR-FTIR spectroscopy to monitor affinity chromatography purification of monoclonal antibodies

Science.gov (United States)

Boulet-Audet, Maxime; Kazarian, Sergei G.; Byrne, Bernadette

2016-01-01

In recent years many monoclonal antibodies (mAb) have entered the biotherapeutics market, offering new treatments for chronic and life-threatening diseases. Protein A resin captures monoclonal antibody (mAb) effectively, but the binding capacity decays over repeated purification cycles. On an industrial scale, replacing fouled Protein A affinity chromatography resin accounts for a large proportion of the raw material cost. Cleaning-in-place (CIP) procedures were developed to extend Protein A resin lifespan, but chromatograms cannot reliably quantify any remaining contaminants over repeated cycles. To study resin fouling in situ, we coupled affinity chromatography and Fourier transform infrared (FTIR) spectroscopy for the first time, by embedding an attenuated total reflection (ATR) sensor inside a micro-scale column while measuring the UV 280 nm and conductivity. Our approach quantified the in-column protein concentration in the resin bed and determined protein conformation. Our results show that Protein A ligand leached during CIP. We also found that host cell proteins bound to the Protein A resin even more strongly than mAbs and that typical CIP conditions do not remove all fouling contaminants. The insights derived from in-column ATR-FTIR spectroscopic monitoring could contribute to mAb purification quality assurance as well as guide the development of more effective CIP conditions to optimise resin lifespan. PMID:27470880

In-column ATR-FTIR spectroscopy to monitor affinity chromatography purification of monoclonal antibodies

Science.gov (United States)

Boulet-Audet, Maxime; Kazarian, Sergei G.; Byrne, Bernadette

2016-07-01

In recent years many monoclonal antibodies (mAb) have entered the biotherapeutics market, offering new treatments for chronic and life-threatening diseases. Protein A resin captures monoclonal antibody (mAb) effectively, but the binding capacity decays over repeated purification cycles. On an industrial scale, replacing fouled Protein A affinity chromatography resin accounts for a large proportion of the raw material cost. Cleaning-in-place (CIP) procedures were developed to extend Protein A resin lifespan, but chromatograms cannot reliably quantify any remaining contaminants over repeated cycles. To study resin fouling in situ, we coupled affinity chromatography and Fourier transform infrared (FTIR) spectroscopy for the first time, by embedding an attenuated total reflection (ATR) sensor inside a micro-scale column while measuring the UV 280 nm and conductivity. Our approach quantified the in-column protein concentration in the resin bed and determined protein conformation. Our results show that Protein A ligand leached during CIP. We also found that host cell proteins bound to the Protein A resin even more strongly than mAbs and that typical CIP conditions do not remove all fouling contaminants. The insights derived from in-column ATR-FTIR spectroscopic monitoring could contribute to mAb purification quality assurance as well as guide the development of more effective CIP conditions to optimise resin lifespan.

Modeling Microalgal Biosediment Formation Based on Attenuated Total Reflection Fourier Transform Infrared (ATR FT-IR) Monitoring.

Science.gov (United States)

Ogburn, Zachary L; Vogt, Frank

2018-03-01

With increasing amounts of anthropogenic pollutants being released into ecosystems, it becomes ever more important to understand their fate and interactions with living organisms. Microalgae play an important ecological role as they are ubiquitous in marine environments and sequester inorganic pollutants which they transform into organic biomass. Of particular interest in this study is their role as a sink for atmospheric CO 2 , a greenhouse gas, and nitrate, one cause of harmful algal blooms. Novel chemometric hard-modeling methodologies have been developed for interpreting phytoplankton's chemical and physiological adaptations to changes in their growing environment. These methodologies will facilitate investigations of environmental impacts of anthropogenic pollutants on chemical and physiological properties of marine microalgae (here: Nannochloropsis oculata). It has been demonstrated that attenuated total reflection Fourier transform infrared (ATR FT-IR) spectroscopy can gain insights into both and this study only focuses on the latter. From time-series of spectra, the rate of microalgal biomass settling on top of a horizontal ATR element is derived which reflects several of phytoplankton's physiological parameters such as growth rate, cell concentrations, cell size, and buoyancy. In order to assess environmental impacts on such parameters, microalgae cultures were grown under 25 different chemical scenarios covering 200-600 ppm atmospheric CO 2 and 0.35-0.75 mM dissolved NO 3 - . After recording time-series of ATR FT-IR spectra, a multivariate curve resolution-alternating least squares (MCR-ALS) algorithm extracted spectroscopic and time profiles from each data set. From the time profiles, it was found that in the considered concentration ranges only NO 3 - has an impact on the cells' physiological properties. In particular, the cultures' growth rate has been influenced by the ambient chemical conditions. Thus, the presented spectroscopic�

Optical fingerprints of solid-liquid interfaces: a joint ATR-IR and first principles investigation

Science.gov (United States)

Yang, L.; Niu, F.; Tecklenburg, S.; Pander, M.; Nayak, S.; Erbe, A.; Wippermann, S.; Gygi, F.; Galli, G.

Despite the importance of understanding the structural and bonding properties of solid-liquid interfaces for a wide range of (photo-)electrochemical applications, there are presently no experimental techniques available to directly probe the microscopic structure of solid-liquid interfaces. To develop robust strategies to interpret experiments and validate theory, we carried out attenuated total internal reflection (ATR-IR) spectroscopy measurements and ab initio molecular dynamics (AIMD) simulations of the vibrational properties of interfaces between liquid water and well-controlled prototypical semiconductor substrates. We show the Ge(100)/H2O interface to feature a reversible potential-dependent surface phase transition between Ge-H and Ge-OH termination. The Si(100)/H2O interface is proposed as a model system for corrosion and oxidation processes. We performed AIMD calculations under finite electric fields, revealing different pathways for initial oxidation. These pathways are predicted to exhibit unique spectral signatures. A significant increase in surface specificity can be achieved utilizing an angle-dependent ATR-IR experiment, which allows to detect such signatures at the interfacial layer and consequently changes in the hydrogen bond network. Funding from DOE-BES Grant No. DE-SS0008939 and the Deutsche Forschungsgemeinschaft (RESOLV, EXC 1069) are gratefully acknowledged.

Characterization of Satsuma mandarin (Citrus unshiu Marc.) nectar-to-honey transformation pathway using FTIR-ATR spectroscopy.

Science.gov (United States)

Svečnjak, Lidija; Prđun, Saša; Rogina, Josip; Bubalo, Dragan; Jerković, Igor

2017-10-01

Samples of Satsuma mandarin (Citrus unshiu Marc.) nectar, honey sac content and honey were analyzed by FTIR-ATR spectroscopy and reference methods. The spectral analysis allowed detection of the major chemical constituents in C. unshiu nectar-to-honey transformation pathway thus providing information on the intensity and location of the compositional changes occurring during this process. The preliminary results showed that in average more than one-third of sugar-related nectar-to-honey conversion takes place directly in the honey sac; the average sugar content (w/w) was 17.93% (nectar), 47.03% (honey sac) and 79.63% (honey). FTIR-ATR results showed great spectral similarity of analyzed honey samples and small degree variations in both sugar and water content in nectar samples. The spectral data revealed distinctive differences in the chemical composition of individual honey sac contents with the most intensive and complex absorption envelope in the spectral region between 1175 and 950cm -1 (glucose, fructose and sucrose absorption bands). Copyright © 2017 Elsevier Ltd. All rights reserved.

Encefalomenigocele atrésico parietal Parietal atresic encephalomeningocele

Directory of Open Access Journals (Sweden)

Liliana Rivera Oliva

2011-09-01

Full Text Available El encefalocele es una anomalía congénita rara, en la que una porción del encéfalo protruye a través de un orificio craneal (evaginación, generalmente situado en la línea media. Clínicamente se caracteriza por una masa epicraneal, de consistencia blanda, muchas veces acompañada de trastornos psicomotores, convulsiones y trastornos de la visión. Se presenta el caso de un recién nacido con diagnóstico de encefalomeningocele atrésico parietal, intervenido quirúrgicamente y con evolución satisfactoria.The encephalocele is a uncommon congenital anomaly where a portion of encephalon protrudes through a cranial orifice (evagination, generally located in the middle line. Clinically, it is characterized by a soft epicranial mass often accompanied or psychomotor disorders, convulsions and vision disorders. This is the case of a newborn diagnosed with parietal atresic encephalomeningocele operated on with a satisfactory evolution.

Measured thermal and fast neutron fluence rates for ATF-1 holders during ATR cycle 158B/159A

Energy Technology Data Exchange (ETDEWEB)

Smith, Larry Don [Idaho National Lab. (INL), Idaho Falls, ID (United States); Miller, David Torbet [Idaho National Lab. (INL), Idaho Falls, ID (United States); Walker, Billy Justin [Idaho National Lab. (INL), Idaho Falls, ID (United States)

2016-11-01

This report contains the thermal (2200 m/s) and fast (E>1MeV) neutron fluence rate data for the ATF-1 holders located in core for ATR Cycle 158B/159A which were measured by the Radiation Measurements Laboratory (RML).

Development of C-lignin with G/S-lignin and lipids in orchid seed coats – an unexpected diversity exposed by ATR-FT-IR spectroscopy

DEFF Research Database (Denmark)

Barsberg, Søren Talbro; Lee, Y.-I.; Rasmussen, Hanne Nina

2018-01-01

Cite this article: Barsberg ST, Lee Y-I, Rasmussen HN. Development of C-lignin with G/S-lignin and lipids in orchid seed coats – an unexpected diversity exposed by ATR-FT-IR spectroscopy. Seed Science Research https:// doi.org/10.1017/S0960258517000344......Cite this article: Barsberg ST, Lee Y-I, Rasmussen HN. Development of C-lignin with G/S-lignin and lipids in orchid seed coats – an unexpected diversity exposed by ATR-FT-IR spectroscopy. Seed Science Research https:// doi.org/10.1017/S0960258517000344...

Rapid quantification of TBP and TBP degradation product ratios by FTIR-ATR

International Nuclear Information System (INIS)

Gillens, A.R.; Powell, B.A.; Clemson University, Clemson, SC

2013-01-01

Tri-n-butyl phosphate (TBP) is the key complexant within the plutonium and uranium reduction extraction process used to extract uranium and plutonium from used nuclear fuel. During reprocessing TBP degrades to dibutyl phosphate (DBP), butyl acid phosphate (MBP), butanol, and phosphoric acid over time. A method for rapidly monitoring TBP degradation is needed for the support of nuclear forensics. Therefore, a Fourier transform infrared spectrometry-attenuated total reflectance (FTIR-ATR) technique was developed to determine approximate peak intensity ratios of TBP and its degradation products. The technique was developed by combining variable concentrations of TBP, DBP, and MBP to simulate TBP degradation. This method is achieved by analyzing selected peak positions and peak intensity ratios of TBP and DBP at different stages of degradation. The developed technique was tested on TBP samples degraded with nitric acid. In mock degradation samples, the 1,235 cm -1 peak position shifts to 1,220 cm -1 as the concentration of TBP decreases and DBP increases. Peak intensity ratios of TBP positions at 1,279 and 1,020 cm -1 relative to DBP positions at 909 and 1,003 cm -1 demonstrate an increasing trend as the concentration of DBP increases. The same peak intensity ratios were used to analyze DBP relative to MBP whereas a decreasing trend is seen with increasing DBP concentrations. The technique developed from this study may be used as a tool to determine TBP degradation in nuclear reprocessing via a rapid FTIR-ATR measurement without gas chromatography analysis. (author)

In-line ATR-UV and Raman Spectroscopy for Monitoring API Dissolution Process During Liquid-Filled Soft-Gelatin Capsule Manufacturing.

Science.gov (United States)

Wan, Boyong; Zordan, Christopher A; Lu, Xujin; McGeorge, Gary

2016-10-01

Complete dissolution of the active pharmaceutical ingredient (API) is critical in the manufacturing of liquid-filled soft-gelatin capsules (SGC). Attenuated total reflectance UV spectroscopy (ATR-UV) and Raman spectroscopy have been investigated for in-line monitoring of API dissolution during manufacturing of an SGC product. Calibration models have been developed with both techniques for in-line determination of API potency. Performance of both techniques was evaluated and compared. The ATR-UV methodology was found to be able to monitor the dissolution process and determine the endpoint, but was sensitive to temperature variations. The Raman technique was also capable of effectively monitoring the process and was more robust to the temperature variation and process perturbations by using an excipient peak for internal correction. Different data preprocessing methodologies were explored in an attempt to improve method performance.

ATR-FTIR Spectroscopy on intact dried leaves of sage (Salvia officinalis L. – chemotaxonomic discrimination and essential oil composition

Directory of Open Access Journals (Sweden)

Gudi, Gennadi

2016-07-01

Full Text Available Sage (Salvia officinalis L. is cultivated worldwide for its aromatic leaves which are used as herbal spice and for phytopharmaceutical applications. Fast analytical strategies for essential oil analysis, performed directly on plant material would reduce the delay between sampling and analytical results. This would enhance product quality by improving technical control of cultivation. The attenuated total reflectance Fourier transform infrared (ATR-FTIR spectroscopy method described here provides a reliable calibration model for quantification of essential oil components (EOC and its main constituents (e.g. -thujone and -thujone directly on dried, intact leaves of sage. Except for drying no further sample preparation is required for ATR-FTIR and the measurement time of less than 5 min per sample contrasts with the most common alternative of hydro-distillation followed by GC analysis which can take several hours per sample.

Glycation in human fingernail clippings using ATR-FTIR spectrometry, a new marker for the diagnosis and monitoring of diabetes mellitus.

Science.gov (United States)

Coopman, Renaat; Van de Vyver, Thijs; Kishabongo, Antoine Sadiki; Katchunga, Philippe; Van Aken, Elisabeth H; Cikomola, Justin; Monteyne, Tinne; Speeckaert, Marijn M; Delanghe, Joris R

2017-01-01

Although HbA1c is a good diagnostic tool for diabetes, the precarity of the health system and the costs limit the use of this biomarker in developing countries. Fingernail clippings contain ±85% of keratins, which are prone to glycation. Nail keratin glycation may reflect the average glycemia over the last months. We explored if attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR) can be used as a non-invasive tool for assessing glycation in diabetes. Using ATR-FTIR spectroscopy, glycation and deglycation experiments with fructosamine 3-kinase allowed to identify the spectrum that corresponds with keratin glycation in fingernail clippings. Clippings of 105 healthy subjects and 127 diabetics were subjected to the standardized ATR-FTIR spectroscopy method. In vitro glycation resulted in an increased absorption at 1047cm -1 . Following enzymatic deglycation, this peak diminished significantly, proving that the AUC between 970 and 1140cm -1 corresponded with glycated proteins. Within-run CV of the assay was 3%. Storage of nail clippings at 37°C for 2weeks did not significantly change results. In diabetics, glycated nail protein concentrations (median: 1.51μmol/g protein, IQR: 1.37-1.85μmol/g protein) were significantly higher than in the controls (median: 1.19μmol/g protein, IQR: 1.09-1.26μmol/g protein) (p<0.0001). ROC analysis yielded an AUC of 0.92 at a cut-off point of 1.28μmol/g nail (specificity: 82%; sensitivity: 90%). No correlation was observed between the glycated nail protein concentrations and HbA1c. Protein glycation analysis in fingernails with ATR-FTIR spectroscopy could be an alternative affordable technique for diagnosing and monitoring diabetes. As the test does not consume reagents, and the preanalytical phase is extremely robust, the test could be particularly useful in developing countries. Copyright © 2016 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.

Overview of MOX fuel fabrication achievements

International Nuclear Information System (INIS)

Bairiot, H.; Vliet, J. van; Chiarelli, G.; Edwards, J.; Nagai, S.H.; Reshetnikov, F.

2000-01-01

Such overview having been adequately covered in an OECD/NEA publication providing the situation as of end 1994, this paper is mainly devoted to an update as of end 1998. The Belgian plant, Belgonucleaire/Dessel, is now dedicated exclusively to the fabrication of MOX fuel and has operated consistently around its nameplate capacity (35tHM/a) through the 1990s involving a large variety of PWR and BWR fuels. The two French plants have also achieved routine operation during the 1990s. CFCa, historically the largest FBR MOX fuel manufacturer, is utilizing the genuine COCA process for that type of fuel and the MIMAS process for LWR fuel: a nominal capacity (40 tHM/a) has been gradually approached. MELOX has operated at 100 tHM/a, as defined in the operating licence granted originally. The British plant, MDF/Sellafield with 8tHM/a nameplate capacity is devoted to fuel and has manufactured several small fabrication campaigns. In Japan, JNC operates three facilities located at Tokai: PFDF, devoted to basic research and fabrication of test fuels, PFFF/ATR line, for the fabrication of Fugen fuel and of corresponding fuel for the critical facility DCA, and PFPF for the fabrication of FBR fuel. In Russia, fabrication techniques have been developed to fuel four BN-800 FBRs contemplated to be constructed and be fuelled with the civilian Pu stockpile. Two demonstration facilities Paket (Mayak) and RIAR (Dimitrovgrad) fabricated respectively pellet and vipac type FBR MOX fuel for BR-5, BOR-60, BN-350 and BN-600. The paper includes a brief description of each of the fabrication routes mentioned, as well as the production of respectively LWR and FBR MOX fuel in each fabrication facility, since the start-up of the plant, since 1 January 1993 and since 1 January 1998 up to 31 December 1998. (author)

Rapid, nondestructive estimation of surface polymer layer thickness using attenuated total reflection fourier transform infrared (ATR FT-IR) spectroscopy and synthetic spectra derived from optical principles.

Science.gov (United States)

Weinstock, B André; Guiney, Linda M; Loose, Christopher

2012-11-01

We have developed a rapid, nondestructive analytical method that estimates the thickness of a surface polymer layer with high precision but unknown accuracy using a single attenuated total reflection Fourier transform infrared (ATR FT-IR) measurement. Because the method is rapid, nondestructive, and requires no sample preparation, it is ideal as a process analytical technique. Prior to implementation, the ATR FT-IR spectrum of the substrate layer pure component and the ATR FT-IR and real refractive index spectra of the surface layer pure component must be known. From these three input spectra a synthetic mid-infrared spectral matrix of surface layers 0 nm to 10,000 nm thick on substrate is created de novo. A minimum statistical distance match between a process sample's ATR FT-IR spectrum and the synthetic spectral matrix provides the thickness of that sample. We show that this method can be used to successfully estimate the thickness of polysulfobetaine surface modification, a hydrated polymeric surface layer covalently bonded onto a polyetherurethane substrate. A database of 1850 sample spectra was examined. Spectrochemical matrix-effect unknowns, such as the nonuniform and molecularly novel polysulfobetaine-polyetherurethane interface, were found to be minimal. A partial least squares regression analysis of the database spectra versus their thicknesses as calculated by the method described yielded an estimate of precision of ±52 nm.

Major Roles for Pyrimidine Dimers, Nucleotide Excision Repair, and ATR in the Alternative Splicing Response to UV Irradiation

Directory of Open Access Journals (Sweden)

Manuel J. Muñoz

2017-03-01

Full Text Available We have previously found that UV irradiation promotes RNA polymerase II (RNAPII hyperphosphorylation and subsequent changes in alternative splicing (AS. We show now that UV-induced DNA damage is not only necessary but sufficient to trigger the AS response and that photolyase-mediated removal of the most abundant class of pyrimidine dimers (PDs abrogates the global response to UV. We demonstrate that, in keratinocytes, RNAPII is the target, but not a sensor, of the signaling cascade initiated by PDs. The UV effect is enhanced by inhibition of gap-filling DNA synthesis, the last step in the nucleotide excision repair pathway (NER, and reduced by the absence of XPE, the main NER sensor of PDs. The mechanism involves activation of the protein kinase ATR that mediates the UV-induced RNAPII hyperphosphorylation. Our results define the sequence UV-PDs-NER-ATR-RNAPII-AS as a pathway linking DNA damage repair to the control of both RNAPII phosphorylation and AS regulation.

Wind Energy Japan

Energy Technology Data Exchange (ETDEWEB)

Komatsubara, Kazuyo [Embassy of the Kingdom of the Netherlands, Tokyo (Japan)

2012-06-15

An overview is given of wind energy in Japan: Background; Wind Energy in Japan; Japanese Wind Energy Industry; Government Supports; Useful Links; Major Japanese Companies; Profiles of Major Japanese Companies; Major Wind Energy Projects in Japan.

Aplicación de las espectroscopias IR/ATR y Raman al estudio de la superficie de vidrios sometidos a molturación

Directory of Open Access Journals (Sweden)

Pérez-Villar, S.

2008-04-01

Full Text Available The grinding process of vítreous silica and a conventional glass has been studied by means of Raman and IR/ATR spectroscopies. By using the obtained spectra, the positions and areas of the different bands have been calculated. The Raman spectra have been analysed using the Polymerisation Index (PI that indicates the depolymerization degree achieved on the particle surface after grinding. On the other hand, the IR/ATR spectra have been used to follow the positions of the symmetric and antisymmetric Si-O-Si bands. It has been observed that both techniques give similar results. These indicate that the depolymerisation degree increases with the grinding process. This depolymerisation is due to the broke of Si-O-Si bonds.

El proceso de molienda de sílice vítrea y un vidrio convencional se ha estudiado mediante espectroscopias IR/ATR y Raman. A partir de los espectros obtenidos para diferentes tamaños de partícula se calcularon tanto las posiciones como las áreas de las bandas que aparecían en cada espectro. Utilizando los espectros Raman se ha hecho uso del denominado Índice de Polimerización (IP el cual es indicativo del grado de despolimerización producido en la superficie de las partículas tras el proceso de molienda. Por otro lado, mediante los espectros IR/ATR y analizando las posiciones de las bandas de tensión simétrica y asimétrica de los enlaces Si-O-Si se ha podido seguir el citado proceso de molturación. Se ha observado, mediante ambas técnicas, como la superficie de las partículas se despolimeriza al disminuir el tamaño de partícula. Esta despolimerización es debida a la rotura de los enlaces Si-O-Si.

Parvovirus B19 NS1 protein induces cell cycle arrest at G2-phase by activating the ATR-CDC25C-CDK1 pathway.

Directory of Open Access Journals (Sweden)

Peng Xu

2017-03-01

Full Text Available Human parvovirus B19 (B19V infection of primary human erythroid progenitor cells (EPCs arrests infected cells at both late S-phase and G2-phase, which contain 4N DNA. B19V infection induces a DNA damage response (DDR that facilitates viral DNA replication but is dispensable for cell cycle arrest at G2-phase; however, a putative C-terminal transactivation domain (TAD2 within NS1 is responsible for G2-phase arrest. To fully understand the mechanism underlying B19V NS1-induced G2-phase arrest, we established two doxycycline-inducible B19V-permissive UT7/Epo-S1 cell lines that express NS1 or NS1mTAD2, and examined the function of the TAD2 domain during G2-phase arrest. The results confirm that the NS1 TAD2 domain plays a pivotal role in NS1-induced G2-phase arrest. Mechanistically, NS1 transactivated cellular gene expression through the TAD2 domain, which was itself responsible for ATR (ataxia-telangiectasia mutated and Rad3-related activation. Activated ATR phosphorylated CDC25C at serine 216, which in turn inactivated the cyclin B/CDK1 complex without affecting nuclear import of the complex. Importantly, we found that the ATR-CHK1-CDC25C-CDK1 pathway was activated during B19V infection of EPCs, and that ATR activation played an important role in B19V infection-induced G2-phase arrest.

Japan 2003

DEFF Research Database (Denmark)

Ørstrup, Finn Rude; Hvass, Sven

2003-01-01

Kompendium udarbejdet til en studierejse til Japan  2003 Kunstakademiets Arkitektskole, Studieafdeling 10......Kompendium udarbejdet til en studierejse til Japan  2003 Kunstakademiets Arkitektskole, Studieafdeling 10...

UV-induced nuclear import of XPA is mediated by importin-α4 in an ATR-dependent manner.

Directory of Open Access Journals (Sweden)

Zhengke Li

Full Text Available Xeroderma pigmentosum Group A (XPA is a crucial factor in mammalian nucleotide excision repair (NER and nuclear import of XPA from the cytoplasm for NER is regulated in cellular DNA damage responses in S-phase. In this study, experiments were carried out to determine the transport mechanisms that are responsible for the UV (ultraviolet-induced nuclear import of XPA. We found that, in addition to the nuclear localization signal (NLS of XPA, importin-α4 or/and importin-α7 are required for the XPA nuclear import. Further investigation indicated that, importin-α4 and importin-α7 directly interacted with XPA in cells. Interestingly, the binding of importin-α4 to XPA was dependent on UV-irradiation, while the binding of importin-α7 was not, suggesting a role for importin-α7 in nuclear translocation of XPA in the absence of DNA damage, perhaps with specificity to certain non-S-phases of the cell-cycle. Consistent with the previous report of a dependence of UV-induced XPA nuclear import on ataxia telangiectasia and Rad3-related protein (ATR in S-phase, knockdown of ATR reduced the amount of XPA interacting with importin-α4. In contrast, the GTPase XPA binding protein 1 (XAB1, previously proposed to be required for XPA nuclear import, showed no effect on the nuclear import of XPA in our siRNA knockdown analysis. In conclusion, our results suggest that upon DNA damage transport adaptor importin-α4 imports XPA into the nucleus in an ATR-dependent manner, while XAB1 has no role in this process. In addition, these findings reveal a potential new therapeutic target for the sensitization of cancer cells to chemotherapy.

Arecoline-induced phosphorylated p53 and p21(WAF1) protein expression is dependent on ATM/ATR and phosphatidylinositol-3-kinase in clone-9 cells.

Science.gov (United States)

Chou, Wen-Wen; Guh, Jinn-Yuh; Tsai, Jung-Fa; Hwang, Chi-Ching; Chiou, Shean-Jaw; Chuang, Lea-Yea

2009-06-01

Betel-quid use is associated with liver cancer whereas its constituent arecoline is cytotoxic, genotoxic, and induces p53-dependent p21(WAF1) protein expression in Clone-9 cells (rat hepatocytes). The ataxia telangiectasia mutated (ATM)/rad3-related (ATR)-p53-p21(WAF1) and the phosphatidylinositol-3-kinase (PI3K)-mammalian target of rapamycin (mTOR) pathways are involved in the DNA damage response and the pathogenesis of cancers. Thus, we studied the role of ATM/ATR and PI3K in arecoline-induced p53 and p21(WAF1) protein expression in Clone-9 cells. We found that arecoline (0.5 mM) activated the ATM/ATR kinase at 30 min. The arecoline-activated ATM/ATR substrate contained p-p53Ser15. Moreover, arecoline only increased the levels of the p-p53Ser6, p-p53Ser15, and p-p53Ser392 phosphorylated p53 isoforms among the known isoforms. ATM shRNA attenuated arecoline-induced p-p53Ser15 and p21(WAF1) at 24 h. Arecoline (0.5 mM) increased phosphorylation levels of p-AktSer473 and p-mTORSer2448 at 30-60 min. Dominant-negative PI3K plasmids attenuated arecoline-induced p21(WAF1), but not p-p53Ser15, at 24 h. Rapamycin attenuated arecoline-induced phosphrylated p-p53Ser15, but not p21(WAF1), at 24 h. ATM shRNA, but not dominant-negative PI3K plasmids, attenuated arecoline-induced p21(WAF1) gene transcription. We conclude that arecoline activates the ATM/ATR-p53-p21(WAF1) and the PI3K/Akt-mTOR-p53 pathways in Clone-9 cells. Arecoline-induced phosphorylated p-p53Ser15 expression is dependent on ATM whereas arecoline-induced p21(WAF1) protein expression is dependent on ATM and PI3K. Moreover, p21(WAF1) gene is transcriptionally induced by arecoline-activated ATM. (c) 2009 Wiley-Liss, Inc.

An ATR-dependent function for the Ddx19 RNA helicase in nuclear R-loop metabolism.

Science.gov (United States)

Hodroj, Dana; Recolin, Bénédicte; Serhal, Kamar; Martinez, Susan; Tsanov, Nikolay; Abou Merhi, Raghida; Maiorano, Domenico

2017-05-02

Coordination between transcription and replication is crucial in the maintenance of genome integrity. Disturbance of these processes leads to accumulation of aberrant DNA:RNA hybrids (R-loops) that, if unresolved, generate DNA damage and genomic instability. Here we report a novel, unexpected role for the nucleopore-associated mRNA export factor Ddx19 in removing nuclear R-loops formed upon replication stress or DNA damage. We show, in live cells, that Ddx19 transiently relocalizes from the nucleopore to the nucleus upon DNA damage, in an ATR/Chk1-dependent manner, and that Ddx19 nuclear relocalization is required to clear R-loops. Ddx19 depletion induces R-loop accumulation, proliferation-dependent DNA damage and defects in replication fork progression. Further, we show that Ddx19 resolves R-loops in vitro via its helicase activity. Furthermore, mutation of a residue phosphorylated by Chk1 in Ddx19 disrupts its interaction with Nup214 and allows its nuclear relocalization. Finally, we show that Ddx19 operates in resolving R-loops independently of the RNA helicase senataxin. Altogether these observations put forward a novel, ATR-dependent function for Ddx19 in R-loop metabolism to preserve genome integrity in mammalian cells. © 2017 The Authors.

Measured thermal and fast neutron fluence rates, ATR Cycle 102-A, 11/28/93 thru 1/16/94

International Nuclear Information System (INIS)

Murray, R.K.; Rogers, J.W.

1994-02-01

This report contains the thermal (2,200 m/s) and fast (E > 1MeV) neutron fluence rate data for ATR Cycle 102-A which were measured by the Radiation Measurements Laboratory (RML) as requested by the Power Reactor Programs (ATR Experiments) Radiation Measurements Work Order. This report contains fluence rate values corresponding to the particular elevations (relative to the 80 ft. core elevation) where the measurements were taken. The data in this report consists of (1) a table of the ATR power history and distribution, (2) a hard copy listing of all thermal and fast neutron fluence rates, (3) plots of both the thermal and fast neutron fluence rates, and (4) a magnetic record (3.5 inch diskette) containing a listing of only the fast neutron fluence rates, their assigned elevations and proper header identification of all monitor positions contained herein. The fluence rates reported are for the average power levels given in the table of power history and distribution. All ''H'' holder monitoring wires for this cycle are 54 inches long. All ''SR'' holder monitor wires for this cycle are 55 inches long. This length allows measurement of the full core region and makes the first count elevation 24.73 inches above core midplane. Due to the safety rod problems in the west lobe, ''BR'' holders were used in the W-1, 2, 3, and 4 positions. All ''BR'' holder monitor wires for this cycle are 56.25 inches long. The distance from the end of the wires to the first count position was 4.25 inches for all wires counted from this cycle

The application of ATR-FTIR spectroscopy and multivariate data analysis to study drug crystallisation in the stratum corneum.

Science.gov (United States)

Goh, Choon Fu; Craig, Duncan Q M; Hadgraft, Jonathan; Lane, Majella E

2017-02-01

Drug permeation through the intercellular lipids, which pack around and between corneocytes, may be enhanced by increasing the thermodynamic activity of the active in a formulation. However, this may also result in unwanted drug crystallisation on and in the skin. In this work, we explore the combination of ATR-FTIR spectroscopy and multivariate data analysis to study drug crystallisation in the skin. Ex vivo permeation studies of saturated solutions of diclofenac sodium (DF Na) in two vehicles, propylene glycol (PG) and dimethyl sulphoxide (DMSO), were carried out in porcine ear skin. Tape stripping and ATR-FTIR spectroscopy were conducted simultaneously to collect spectral data as a function of skin depth. Multivariate data analysis was applied to visualise and categorise the spectral data in the region of interest (1700-1500cm -1 ) containing the carboxylate (COO - ) asymmetric stretching vibrations of DF Na. Spectral data showed the redshifts of the COO - asymmetric stretching vibrations for DF Na in the solution compared with solid drug. Similar shifts were evident following application of saturated solutions of DF Na to porcine skin samples. Multivariate data analysis categorised the spectral data based on the spectral differences and drug crystallisation was found to be confined to the upper layers of the skin. This proof-of-concept study highlights the utility of ATR-FTIR spectroscopy in combination with multivariate data analysis as a simple and rapid approach in the investigation of drug deposition in the skin. The approach described here will be extended to the study of other actives for topical application to the skin. Copyright © 2016 Elsevier B.V. All rights reserved.

Application of fuzzy logic control system for reactor feed-water control

International Nuclear Information System (INIS)

Iijima, T.; Nakajima, Y.

1994-01-01

The successful actual application of a fuzzy logic control system to the a nuclear Fugen nuclear power reactor is described. Fugen is a heavy-water moderated, light-water cooled reactor. The introduction of fuzzy logic control system has enabled operators to control the steam drum water level more effectively in comparison to a conventional proportional-integral (PI) control system

Proteomics Reveals Global Regulation of Protein SUMOylation by ATM and ATR Kinases during Replication Stress

DEFF Research Database (Denmark)

Munk, Stephanie; Sigurðsson, Jón Otti; Xiao, Zhenyu

2017-01-01

The mechanisms that protect eukaryotic DNA during the cumbersome task of replication depend on the precise coordination of several post-translational modification (PTM)-based signaling networks. Phosphorylation is a well-known regulator of the replication stress response, and recently an essentia....... They analyze changes in the SUMO and phosphoproteome after MMC and hydroxyurea treatments and find that the DNA damage response kinases ATR and ATM globally regulate SUMOylation upon replication stress and fork breakage....

The Conference Proceedings of the 2003 Air Transport Research Society (ATRS) World Conference, Volume 3

Science.gov (United States)

Bowen, Brent (Editor); Gudmundsson, Sveinn (Editor); Oum, Tae (Editor)

2003-01-01

Volume 3 of the 2003 Air Transport Reserch Society (ATRS) World Conference includes papers on topics relevant to airline operations worldwide. Specific topics include: European Union and civil aviation regimens;simulating decision making in airline operations, passenger points of view on convenient airports; route monopolies and nonlinear pricing; cooperation among airports in Europe; fleet modernizaiton in Brazil;the effects of deregulation on the growth of air transportation in Europe and the United States.

A Novel Zn2-Cys6 Transcription Factor AtrR Plays a Key Role in an Azole Resistance Mechanism of Aspergillus fumigatus by Co-regulating cyp51A and cdr1B Expressions

Science.gov (United States)

Shimizu, Kiminori; Paul, Sanjoy; Ohba, Ayumi; Gonoi, Tohru; Watanabe, Akira; Gomi, Katsuya

2017-01-01

Successful treatment of aspergillosis caused by Aspergillus fumigatus is threatened by an increasing incidence of drug resistance. This situation is further complicated by the finding that strains resistant to azoles, the major antifungal drugs for aspergillosis, have been widely disseminated across the globe. To elucidate mechanisms underlying azole resistance, we identified a novel transcription factor that is required for normal azole resistance in Aspergillus fungi including A. fumigatus, Aspergillus oryzae, and Aspergillus nidulans. This fungal-specific Zn2-Cys6 type transcription factor AtrR was found to regulate expression of the genes related to ergosterol biosynthesis, including cyp51A that encodes a target protein of azoles. The atrR deletion mutant showed impaired growth under hypoxic conditions and attenuation of virulence in murine infection model for aspergillosis. These results were similar to the phenotypes for a mutant strain lacking SrbA that is also a direct regulator for the cyp51A gene. Notably, AtrR was responsible for the expression of cdr1B that encodes an ABC transporter related to azole resistance, whereas SrbA was not involved in the regulation. Chromatin immunoprecipitation assays indicated that AtrR directly bound both the cyp51A and cdr1B promoters. In the clinically isolated itraconazole resistant strain that harbors a mutant Cyp51A (G54E), deletion of the atrR gene resulted in a hypersensitivity to the azole drugs. Together, our results revealed that AtrR plays a pivotal role in a novel azole resistance mechanism by co-regulating the drug target (Cyp51A) and putative drug efflux pump (Cdr1B). PMID:28052140

Trasmission and Reflection (ATR)Far-Infrared Spectroscopy Applied in the Analysis of Cultural Heritage Materials

OpenAIRE

Kendix, Elsebeth Langholz

2009-01-01

FIR spectroscopy is an alternative way of collecting spectra of many inorganic pigments and corrosion products found on art objects, which is not normally observed in the MIR region. Most FIR spectra are traditionally collected in transmission mode but as a real novelty it is now also possible to record FIR spectra in ATR (Attenuated Total Reflectance) mode. In FIR transmission we employ polyethylene (PE) for preparation of pellets by embedding the sample in PE. Unfortunately, ...

A Feasibility Study to Determine Cooling Time and Burnup of ATR Fuel Using a Nondestructive Technique and Three Types of Gamma-ray Detectors

International Nuclear Information System (INIS)

Navarro, Jorge; Aryaeinejad, Rahmat; Nigg, David W.

2011-01-01

Effective and efficient Advanced Test Reactor (ATR) fuel management require state of the art core modeling tools. These new tools will need isotopic and burnup validation data before they are put into production. To create isotopic, burn up validation libraries and to determine the setup for permanent fuel scanner system a feasibility study was perform. The study consisted in measuring short and long cooling time fuel elements at the ATR canal. Three gamma spectroscopy detectors (HPGe, LaBr3, and HPXe) and two system configurations (above and under water) were used in the feasibility study. The first stage of the study was to investigate which detector and system configuration would be better suited for different scenarios. The second stage of the feasibility study was to create burnup and cooling time calibrations using experimental isotopic data collected and ORIGEN 2.2 burnup data. The results of the study establish that a better spectra resolution is achieve with an above the water configuration and that three detectors can be used in the permanent fuel scanner system for different situations. In addition it was conclude that a number of isotopic ratios and absolute measurements could be used to predict ATR fuel burnup and cooling times.

Overcoming Endocrine Resistance by Targeting ER/FoxA1/IL-8 Axis

Science.gov (United States)

2015-10-01

Creighton, Fugen Li, Martin Shea, Agostina Nardone, Tao Wang, Laura M. Heiser , Pavana Anur, Nicholas Wang, Catie Grasso, Paul Spellman, Carolina...Xiaoyong Fu, Rinath Jeselsohn, Resel Pereira, Emporia F. Hollingsworth, Chad J. Creighton, Fugen Li, Martin Shea, Agostina Nardone, Laura M. Heiser , Pavana...Rinath Jeselsohn, Emporia F. Hollingsworth, Dolores Lopez-Terrada, Chad J. Creighton, Agostina Nardone, Martin Shea, Laura M. Heiser , Pavana Anur

Quantitative analysis of glycated albumin in serum based on ATR-FTIR spectrum combined with SiPLS and SVM.

Science.gov (United States)

Li, Yuanpeng; Li, Fucui; Yang, Xinhao; Guo, Liu; Huang, Furong; Chen, Zhenqiang; Chen, Xingdan; Zheng, Shifu

2018-08-05

A rapid quantitative analysis model for determining the glycated albumin (GA) content based on Attenuated total reflectance (ATR)-Fourier transform infrared spectroscopy (FTIR) combining with linear SiPLS and nonlinear SVM has been developed. Firstly, the real GA content in human serum was determined by GA enzymatic method, meanwhile, the ATR-FTIR spectra of serum samples from the population of health examination were obtained. The spectral data of the whole spectra mid-infrared region (4000-600 cm -1 ) and GA's characteristic region (1800-800 cm -1 ) were used as the research object of quantitative analysis. Secondly, several preprocessing steps including first derivative, second derivative, variable standardization and spectral normalization, were performed. Lastly, quantitative analysis regression models were established by using SiPLS and SVM respectively. The SiPLS modeling results are as follows: root mean square error of cross validation (RMSECV T ) = 0.523 g/L, calibration coefficient (R C ) = 0.937, Root Mean Square Error of Prediction (RMSEP T ) = 0.787 g/L, and prediction coefficient (R P ) = 0.938. The SVM modeling results are as follows: RMSECV T  = 0.0048 g/L, R C  = 0.998, RMSEP T  = 0.442 g/L, and R p  = 0.916. The results indicated that the model performance was improved significantly after preprocessing and optimization of characteristic regions. While modeling performance of nonlinear SVM was considerably better than that of linear SiPLS. Hence, the quantitative analysis model for GA in human serum based on ATR-FTIR combined with SiPLS and SVM is effective. And it does not need sample preprocessing while being characterized by simple operations and high time efficiency, providing a rapid and accurate method for GA content determination. Copyright © 2018 Elsevier B.V. All rights reserved.

Department of Energy's Advanced Test Reactor (ATR), July 14--18, 1980: An independent on-site safety review

International Nuclear Information System (INIS)

1981-02-01

The intent of this review was not to conduct a detailed in-depth audit, but rather to make a broad management assessment of ATR operations. The results of the review should only be considered as having identified trends or indications. The Team's observations and recommendations for the most part are based upon standards used for licensed reactor facility practices. These standards form the basis for many of the comments in this report. The Team believes that a uniform minimum standard of performance should be achieved in the operation of DOE reactors. In order to assure that this is accomplished, clear standards are necessary. Consistent with the past AEC and ERDA policy, the Team has used the standards of the commercial nuclear power industry. It is recognized that this approach is conservative, in that the ATR reactor has a significantly greater degree of inherent safety (lower pressure, temperature, power, etc.) than a licensed reactor. Although the Review Team found no indications or evidence that the plant is being operated in an unsafe manner, various areas were identified where improvements are either needed or should be considered to increase the safety of reactor operations

Networking Japan

DEFF Research Database (Denmark)

Hansen, Annette Skovsted

Human Resource Development was the first and remains an important pillar in Japanese foreign aid. I will argue that Japan has access to a global network of alumni who will co-define Japanese foreign aid in the future, because Japan has encouraged alumni societies and networking since 1965. A total...... of more than a million people in more than 100 countries have attended courses in Japan funded fully or partly by Japanese ODA since the inception of the technical assistance programs in 1954 through the Colombo Plan and since 1959 through the Association of Overseas Technical Scholarships (AOTS from 2009...... HIDA). Many of these alumni have and will in the future exchange ideas and keep contact not only to Japan, but also to fellow alumni around the globe and, thereby, practice south-south exchanges, which are made possible and traceable by their established alumni network and the World Network of Friends...

IRBA SERIES : Accounting In Japan

OpenAIRE

Arai, Kiyomitsu

1994-01-01

Preface / Legal and Conceptual Framework of Accounting in Japan / Setting Accounting Standards in Japan - The American Influence and The Present Status / Accounting Education and Profession in Japan / The International Harmonization of Accounting Standards / The Accounting Standard Setting in Japan and Its Responses to International Accounting Standards / Selected Bibliography for Accounting in Japan

Characterization of southern yellow pine bark layers by Attenuated Total Reflectance (ATR) and Fourier Transform Infrared (FT-IR) Spectroscopy

Science.gov (United States)

Thomas L. Eberhardt

2009-01-01

The outer bark (rhytidome) of the southern yellow pines is a complex structure comprised of alternating layers of obliterated phloem and periderm tissues, with the latter comprised of three layers, those being phellem, phellogen, and phelloderm. An attenuated total reflectance (ATR) sampling accessory, coupled with a Fourier transform infrared (FTIR) spectrometer,...

Measured thermal and fast neutron fluence rates, ATR Cycle 100-BC, April 23, 1993--May 13, 1993

International Nuclear Information System (INIS)

Smith, L.D.; Murray, R.K.; Rogers, J.W.

1993-07-01

This report contains the thermal (2200 m/s) and fast (E>1MeV) neutron fluence rate data for ATR Cycle 100-BC which were measured by the Radiation Measurements Laboratory (RML) as requested by the Power Reactor Programs (ATR Experiments) Radiation Measurements Work Order. This report contains fluence rate values corresponding to the particular elevations (relative to the 80 ft. core elevation) where the measurements were taken. The data in this report consists of (1) a table of the ATR power history and distribution, (2) a hard copy listing of all thermal and fast neutron fluence rates, (3) plots of both the thermal and fast neutron fluence rates, and (4) a magnetic record (3.5 inch diskette) containing a listing of only the fast neutron fluence rates, their assigned elevations and proper header identification of all monitor positions contained herein. The fluence rates reported are for the average power levels given in the table of power history and distribution. All open-quotes Hclose quotes holder monitor wires for this cycle are 54 inches long. All open-quotes SRclose quotes holder monitor wires for this cycle are 55 inches long. This length allows measurement of the full core region and makes the first count elevation 24.73 inches above core midplane. Due to the safety rod problems in the west lobe, open-quotes BRclose quotes holders were used in the W-1, 2, 3, and 4 positions. All open-quotes BRclose quotes holder monitor wires for this cycle are 56.25 inches long. The distance from the end of the wires to the first count position was 4.25 inches for all wires counted from this cycle. The results from the measurements in the W-1, 2, 3, 4 monitor positions indicate that the safety rod followers were rotated to a different azimuthal orientation relative to the normal orientation. The results indicate that the rotation was counterclockwise from their normal orientation. This is the same condition observed starting with Cycle 99-B

Small Molecules Targeting Ataxia Telangiectasia and Rad3-Related (ATR) Kinase: An Emerging way to Enhance Existing Cancer Therapy

Czech Academy of Sciences Publication Activity Database

Andrs, M.; Korábečný, J.; Nepovimova, E.; Jun, D.; Hodný, Zdeněk; Kuca, K.

2016-01-01

Roč. 16, č. 3 (2016), s. 200-208 ISSN 1568-0096 Institutional support: RVO:68378050 Keywords : Ataxia telangiectasia and Rad3-related kinase (ATR) * cancer * chemosensitization * DNA damage response * phosphatidylinositol 3-kinase-related protein kinases (PIKK) * radiosensitization * synthetic lethality Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 2.992, year: 2016

Identification of herbarium whole-leaf samples of Epilobium species by ATR-IR spectroscopy.

Science.gov (United States)

Strgulc Krajsek, Simona; Buh, Primoz; Zega, Anamarija; Kreft, Samo

2008-02-01

A simple, high-accuracy FT-IR method based on attenuated total reflection (ATR) was developed for the rapid determination of leaf samples of Epilobium species. The method is superior to other analytical techniques, since there is no need of laborious sample preparation such as grinding or extraction and solvent removal. A total of 70 herbarium specimens, belonging to all 13 Epilobium and to 2 Chamerion species growing in Slovenia, were analyzed. With the 100 most-informative wavenumbers in the range 700-1800 cm(-1), we obtained over 90% accuracy of species identification, with discriminant multivariate statistical analysis on the measurements made on whole dried leaves.

Dynamic dependence on ATR and ATM for double-strand break repair in human embryonic stem cells and neural descendants.

Science.gov (United States)

Adams, Bret R; Golding, Sarah E; Rao, Raj R; Valerie, Kristoffer

2010-04-02

The DNA double-strand break (DSB) is the most toxic form of DNA damage. Studies aimed at characterizing DNA repair during development suggest that homologous recombination repair (HRR) is more critical in pluripotent cells compared to differentiated somatic cells in which nonhomologous end joining (NHEJ) is dominant. We have characterized the DNA damage response (DDR) and quality of DNA double-strand break (DSB) repair in human embryonic stem cells (hESCs), and in vitro-derived neural cells. Resolution of ionizing radiation-induced foci (IRIF) was used as a surrogate for DSB repair. The resolution of gamma-H2AX foci occurred at a slower rate in hESCs compared to neural progenitors (NPs) and astrocytes perhaps reflective of more complex DSB repair in hESCs. In addition, the resolution of RAD51 foci, indicative of active homologous recombination repair (HRR), showed that hESCs as well as NPs have high capacity for HRR, whereas astrocytes do not. Importantly, the ATM kinase was shown to be critical for foci formation in astrocytes, but not in hESCs, suggesting that the DDR is different in these cells. Blocking the ATM kinase in astrocytes not only prevented the formation but also completely disassembled preformed repair foci. The ability of hESCs to form IRIF was abrogated with caffeine and siRNAs targeted against ATR, implicating that hESCs rely on ATR, rather than ATM for regulating DSB repair. This relationship dynamically changed as cells differentiated. Interestingly, while the inhibition of the DNA-PKcs kinase (and presumably non-homologous endjoining [NHEJ]) in astrocytes slowed IRIF resolution it did not in hESCs, suggesting that repair in hESCs does not utilize DNA-PKcs. Altogether, our results show that hESCs have efficient DSB repair that is largely ATR-dependent HRR, whereas astrocytes critically depend on ATM for NHEJ, which, in part, is DNA-PKcs-independent.

Investigation of ATR-FTIR spectroscopy as an alternative to the Water Leach Free Acidity test for cellulose acetate-based film

DEFF Research Database (Denmark)

Johansen, Karin Bonde; Shashoua, Yvonne

2005-01-01

Cellulose acetate film loses acetate groups on ageing which results in the formation of damaging acetic acid. Water-Leach Free Acidity Test (WLFAT) is the definitive technique to quantify acidity, but requires 1g film and 26 hours. ATR-FTIR spectroscopy is a non-destructive, rapid technique which...

Reconsidering Japan's underperformance in pharmaceuticals: evidence from Japan's anticancer drug sector.

Science.gov (United States)

Umemura, Maki

2010-01-01

Unlike its automobile or electronics industries, Japan's pharmaceutical industry did not become a global leader. Japan remains a net importer of pharmaceuticals and has introduced few global blockbuster drugs. Alfred Chandler argued that Japan's pharmaceutical firms remained relatively weak because Western firms enjoyed an insurmountable first first-mover advantage. However, this case study of the anticancer drug sector illustrates that Chandler's explanation is incomplete. Japanese medical culture, government policy, and research environment also played a substantial role in shaping the industry. In the 1970s and 1980s, these factors encouraged firms to develop little few effective drugs with low side effects, and profit from Japan's domestic market. But, these drugs were unsuitable to foreign markets with more demanding efficacy standards. As a result, Japan not only lost more than a decade in developing ineffective drugs, but also neglected to create the infrastructure necessary to develop innovative drugs and build a stronger pharmaceutical industry.

International development of Japan's Nuclear Industry. Indispensable Japan-U.S. cooperation

International Nuclear Information System (INIS)

Saigo, Masao

2006-01-01

It is significant to internationally develop the nuclear power plants technology that has been fostered by Japan's nuclear industry. It is also important to work with taking the degree of development of nuclear power plants of the recipient country into consideration. ''Forum on International Development of Nuclear Industry'' organized by the Japan Atomic Industrial Forum, Inc. (JAIF) proposed it would be indispensable for a Japan's nuclear industry to establish a Japan-U.S. Cooperation with the support of Government in order to develop the nuclear technology internationally. In November 2005, the investigating team including utilities and nuclear industry visited U.S. and exchanged opinions on its possibility. Investigating results and their evaluation were described. (T.Tanaka)

21 CFR 186.1555 - Japan wax.

Science.gov (United States)

2010-04-01

... 21 Food and Drugs 3 2010-04-01 2009-04-01 true Japan wax. 186.1555 Section 186.1555 Food and Drugs... Substances Affirmed as GRAS § 186.1555 Japan wax. (a) Japan wax (CAS Reg. No. 8001-39-6), also known as Japan... fruits of the oriental sumac, Rhus succedanea (Japan, Taiwan, and Indo-China), R. vernicifera (Japan...

Annual report of the Japan Atomic Energy Research Institute, Apr. 1987 - Mar. 1988

International Nuclear Information System (INIS)

1988-01-01

In fiscal 1987, the Atomic Energy Commission of Japan revised the 'Long term program for development and utilization of nuclear energy', which is done every five years. It determined the future direction of the research and development in JAERI. Three basic objectives are indicated in it, that is, to qualitatively upgrade nuclear technology, to tackle the frontiers of the technology, and to promote international collaboration. The new 'Long term task plan' of JAERI based on the 'Long term program' was established in August, 1987. The integral experiment on loss of coolant accident, the test on reactivity-initiated accident, the irradiation and post-irradiation examination of fuel, the development of safety analysis codes, the design and safety analysis of the Nuclear Fuel Cycle Safety Engineering Research Facility, the treatment and disposal of radioactive wastes, the preparatory work for constructing the High Temperature Engineering Test Reactor, the full scale plasma heating experiment with JT-60, the study on high efficiency confinement mode and current drive, the construction of the new home port for the nuclear ship 'Mutsu', the design of an advanced marine reactor, the reactor physics of FBRs and ATRs and other fundamental studies, and the operation of JRR-2, JRR-4 and JMTR are reported. (K.I.)

New particle formation events measured on board the ATR-42 aircraft during the EUCAARI campaign

Directory of Open Access Journals (Sweden)

S. Crumeyrolle

2010-07-01

Full Text Available Aerosol properties were studied during an intensive airborne measurement campaign that took place at Rotterdam in Netherlands in May 2008 within the framework of the European Aerosol Cloud Climate and Air Quality Interactions project (EUCAARI. The objective of this study is to illustrate seven events of new particle formation (NPF observed with two Condensation Particle Counters (CPCs operated on board the ATR-42 research aircraft in airsectors around Rotterdam, and to provide information on the spatial extent of the new particle formation phenomenon based on 1-s resolution measurements of ultra-fine particle (in the size range 3–10 nm diameter, denoted N_3-10 hereafter concentrations. The results show that particle production occurred under the influence of different air mass origins, at different day times and over the North Sea as well as over the continent. The number concentration of freshly nucleated particles (N_3-10 varied between 5000 and 100 000 cm⁻³ within the boundary layer (BL. Furthermore the vertical extension for all nucleation events observed on the ATR-42 never exceeded the upper limit of the BL. The horizontal extent of N_3-10 could not be delimited due to inflexible flight plans which could not be modified to accommodate real-time results. However, the NPF events were observed over geographically large areas; typically the horizontal extension was about 100 km and larger.

Galiellalactone induces cell cycle arrest and apoptosis through the ATM/ATR pathway in prostate cancer cells.

Science.gov (United States)

García, Víctor; Lara-Chica, Maribel; Cantarero, Irene; Sterner, Olov; Calzado, Marco A; Muñoz, Eduardo

2016-01-26

Galiellalactone (GL) is a fungal metabolite that presents antitumor activities on prostate cancer in vitro and in vivo. In this study we show that GL induced cell cycle arrest in G2/M phase, caspase-dependent apoptosis and also affected the microtubule organization and migration ability in DU145 cells. GL did not induce double strand DNA break but activated the ATR and ATM-mediated DNA damage response (DDR) inducing CHK1, H2AX phosphorylation (fH2AX) and CDC25C downregulation. Inhibition of the ATM/ATR activation with caffeine reverted GL-induced G2/M cell cycle arrest, apoptosis and DNA damage measured by fH2AX. In contrast, UCN-01, a CHK1 inhibitor, prevented GL-induced cell cycle arrest but enhanced apoptosis in DU145 cells. Furthermore, we found that GL did not increase the levels of intracellular ROS, but the antioxidant N-acetylcysteine (NAC) completely prevented the effects of GL on fH2AX, G2/M cell cycle arrest and apoptosis. In contrast to NAC, other antioxidants such as ambroxol and EGCG did not interfere with the activity of GL on cell cycle. GL significantly suppressed DU145 xenograft growth in vivo and induced the expression of fH2AX in the tumors. These findings identify for the first time that GL activates DDR in prostate cancer.

ATR FT-IR spectroscopy on Vmh2 hydrophobin self-assembled layers for Teflon membrane bio-functionalization

International Nuclear Information System (INIS)

Portaccio, M.; Gravagnuolo, A.M.; Longobardi, S.; Giardina, P.; Rea, I.; De Stefano, L.; Cammarota, M.; Lepore, M.

2015-01-01

Graphical abstract: - Highlights: • Hydrophobin self-assembled layers on Teflon in different preparation conditions were investigated. • ATR collection data geometry allowed samples examination without any particular preparation. • Amide content, lipid/amide and carbohydrate/amide ratios of the protein layer were estimated. • Secondary structure of protein was determined for the examined samples. • FT-IR demonstrated to be of extreme relevance in monitoring hydrophobin self-assembled layers preparation. - Abstract: Surface functionalization by layers of hydrophobins, amphiphilic proteins produced by fungi offers a promising and green strategy for fabrication of biomedical and bioanalytical devices. The layering process of the Vmh2 hydrophobin from Pleurotus ostreatus on Teflon membrane has been investigated by Attenuated Total Reflection Fourier Transform Infrared (ATR FT-IR) spectroscopy. In particular, protein layers obtained with hydrophobin purified with two different procedures and in various coating conditions have been examined. The layers have been characterized by quantifying the amide I and amide II band area together with the lipid/amide ratio and carbohydrate/amide ratio. This characterization can be very useful in evaluating the best purification strategy and coating conditions. Moreover the analysis of the secondary structure of the layered protein using the deconvolution procedure of amide I band indicate the prevalent contribution from β-sheet state. The results inferred by infrared spectroscopy have been also confirmed by scanning electron microscopy imaging

ATR FT-IR spectroscopy on Vmh2 hydrophobin self-assembled layers for Teflon membrane bio-functionalization

Energy Technology Data Exchange (ETDEWEB)

Portaccio, M., E-mail: marianna.portaccio@unina2.it [Dipartimento di Medicina Sperimentale – Seconda Università di Napoli, Via S.M. di Costantinopoli, 16-80134 Napoli (Italy); Gravagnuolo, A.M., E-mail: alfredomaria.gravagnuolo@unina.it [Dipartimento di Scienze Chimiche, Università “Federico II”, Via Cintia, 21- 80126 Napoli (Italy); Longobardi, S., E-mail: sara.longobardi@unina.it [Dipartimento di Scienze Chimiche, Università “Federico II”, Via Cintia, 21- 80126 Napoli (Italy); Giardina, P., E-mail: paola.giardina@unina.it [Dipartimento di Scienze Chimiche, Università “Federico II”, Via Cintia, 21- 80126 Napoli (Italy); Rea, I., E-mail: ilaria.rea@na.imm.cnr.it [Institute for Microelectronics and Microsystems, CNR, Via P. Castellino, 111-80131 Napoli (Italy); De Stefano, L., E-mail: luca.destefano@na.imm.cnr.it [Institute for Microelectronics and Microsystems, CNR, Via P. Castellino, 111-80131 Napoli (Italy); Cammarota, M., E-mail: marcella.cammarota@unina2.it [Dipartimento di Medicina Sperimentale – Seconda Università di Napoli, Via S.M. di Costantinopoli, 16-80134 Napoli (Italy); Lepore, M., E-mail: maria.lepore@unina2.it [Dipartimento di Medicina Sperimentale – Seconda Università di Napoli, Via S.M. di Costantinopoli, 16-80134 Napoli (Italy)

2015-10-01

Graphical abstract: - Highlights: • Hydrophobin self-assembled layers on Teflon in different preparation conditions were investigated. • ATR collection data geometry allowed samples examination without any particular preparation. • Amide content, lipid/amide and carbohydrate/amide ratios of the protein layer were estimated. • Secondary structure of protein was determined for the examined samples. • FT-IR demonstrated to be of extreme relevance in monitoring hydrophobin self-assembled layers preparation. - Abstract: Surface functionalization by layers of hydrophobins, amphiphilic proteins produced by fungi offers a promising and green strategy for fabrication of biomedical and bioanalytical devices. The layering process of the Vmh2 hydrophobin from Pleurotus ostreatus on Teflon membrane has been investigated by Attenuated Total Reflection Fourier Transform Infrared (ATR FT-IR) spectroscopy. In particular, protein layers obtained with hydrophobin purified with two different procedures and in various coating conditions have been examined. The layers have been characterized by quantifying the amide I and amide II band area together with the lipid/amide ratio and carbohydrate/amide ratio. This characterization can be very useful in evaluating the best purification strategy and coating conditions. Moreover the analysis of the secondary structure of the layered protein using the deconvolution procedure of amide I band indicate the prevalent contribution from β-sheet state. The results inferred by infrared spectroscopy have been also confirmed by scanning electron microscopy imaging.

Revisiting the Brønsted acid catalysed hydrolysis kinetics of polymeric carbohydrates in ionic liquids by in situ ATR-FTIR spectroscopy

DEFF Research Database (Denmark)

Kunov-Kruse, Andreas Jonas; Riisager, Anders; Shunmugavel, Saravanamurugan

2013-01-01

A new versatile method to measure rates and determine activation energies for the Brønsted acid catalysed hydrolysis of cellulose and cellobiose (and other polymeric carbohydrates) in ionic liquids is demonstrated by following the C–O stretching band of the glycoside bond with in situ ATR...

Development of decommissioning technologies in Sumitomo Mitsui Construction Co., Ltd

International Nuclear Information System (INIS)

Maruyama, Shinichiro; Suzuki, Toru; Ogane, Daisuke

2011-01-01

The decommissioning program of nuclear reactors in Japan first started in December 2001 on the Japan's first commercial nuclear power station Tokai Power Plant. In February 2008, the decommissioning of 'Fugen' was first approved as the program on a large-scale water reactor in Japan, and was started. From now on, decommissioning programs of LWRs constructed in the early stage of nuclear development will gradually increase. Decommissioning projects are required more than 20 years for completing the entire processes, because of its characteristics to placing the utmost priority to safety. Diverse types of element technologies are fully utilized in decommissioning projects, such as technology of evaluating remaining radioactivity, decontamination, dismantling/remote control, and treatment/disposal/recycling. Also there are a lot of civil engineering or building technologies and its applied technologies in these element technologies. Sumitomo Mitsui Construction Co., Ltd. has been committed to contributing to the promotion of decommissioning projects in Japan, and has carried out investigation/evaluation of applicability of the existing dismantling technologies to dismantling of reactors, seismic evaluation of the buildings for dismantling the reactor zone, development of recycling of concrete, and discussion of rational waste treatment/disposal methods. In this thesis, we present our decommissioning technologies focusing on the element technologies that our company has investigated and developed so far. (author)

Cutaneous approach towards clinical and pathophysiological aspects of hyperglycemia by ATR FTIR spectroscopy

Science.gov (United States)

Eikje, Natalja Skrebova; Sota, Takayuki; Aizawa, Katsuo

2007-07-01

Attempts were made to non-invasively detect glucose-specific spectral signals in the skin by ATR-FTIR spectroscopy. In vivo spectra were collected from the inner wrists of healthy, prediabetes and diabetes subjects in the 750-4000 cm -1 region, with a closer assessment of the glucose-related region between 1000 and 1180 cm -1. Spectra in vivo showed glucose-specific peaks at 1030, 1080, 1118 and 1151 cm -1, as a variety of glucose solutions are found in vitro. Based on the differences of intensities at 1030 and 1118 cm -1 two spectral patterns were seen: I 1118 > I 1030 for a diabetes and I 1030> I 1118 for non-diabetes subjects. The peak at 1030 cm -1 was used to assess glucose concentrations in the skin due to its good correlation with glucose concentrations in vitro. Calculated mean values of the peak at 1030 cm -1 showed evidence of correlation with blood glucose levels when grouped as = 200 mg/dL, though there was no constant correlation between them when compared before/after OGTT or at the fasting/postprandial states. Absorbances at 1030 cm -1 were not only increased in a dose-dependent manner in a diabetes patient, but were also generally higher than in non-diabetes subjects at 30 min OGTT assessment. Also we could monitor absorbances at 1030 cm -1 and determine their changes in the skin tissue at different times of OGTT. We assume that our approach to in vivo measurement and monitoring of glucose concentrations at 1030 cm -1 may be one of the indicators to assess glucose activity level and its changes in the skin tissue, and has further implications in the study of clinical and pathophysiological aspects of hyperglycemia in diabetes and non-diabetes subjects by ATR-FTIR spectroscopy.

Water channel reactor fuels and fuel channels: Design, performance, research and development. Proceedings of a technical committee meeting

Energy Technology Data Exchange (ETDEWEB)

NONE

1998-01-01

The International Working Group on Water Reactor Fuel Performance and Technology (IWGFPT) recommended holding a Technical Committee Meeting on Water Channel Reactor Fuel including into this category fuels and pressure tubes/fuel channels for Atucha-I and II, BWR, CANDU, FUGEN and RBMK reactors. The IWGFPT considered that even if the characteristics of Atucha, CANDUs, BWRs, FUGEN and RBMKs differ considerably, there are also common features. These features include materials aspects, as well as core, fuel assembly and fuel rod design, and some safety issues. There is also some similarity in fuel power history and operating conditions (Atucha-I and II, FUGEN and RBMK). Experts from 11 countries participated at the meeting and presented papers on technology, performance, safety and design, and materials aspects of fuels and pressure tubes/fuel channels for the above types of water channel reactors. Refs, figs, tabs.

Water channel reactor fuels and fuel channels: Design, performance, research and development. Proceedings of a technical committee meeting

International Nuclear Information System (INIS)

1998-01-01

The International Working Group on Water Reactor Fuel Performance and Technology (IWGFPT) recommended holding a Technical Committee Meeting on Water Channel Reactor Fuel including into this category fuels and pressure tubes/fuel channels for Atucha-I and II, BWR, CANDU, FUGEN and RBMK reactors. The IWGFPT considered that even if the characteristics of Atucha, CANDUs, BWRs, FUGEN and RBMKs differ considerably, there are also common features. These features include materials aspects, as well as core, fuel assembly and fuel rod design, and some safety issues. There is also some similarity in fuel power history and operating conditions (Atucha-I and II, FUGEN and RBMK). Experts from 11 countries participated at the meeting and presented papers on technology, performance, safety and design, and materials aspects of fuels and pressure tubes/fuel channels for the above types of water channel reactors

A new Density Functional Theory (DFT) based method for supporting the assignment of vibrational signatures of mannan and cellulose—Analysis of palm kernel cake hydrolysis by ATR-FT-IR spectroscopy as a case study

DEFF Research Database (Denmark)

Barsberg, Søren Talbro; Sanadi, Anand Ramesh; Jørgensen, Henning

2011-01-01

Attenuated Total Reflectance (ATR) FT-IR spectroscopy gives in situ information on molecular concentration, organization and interactions in plant cell walls. We demonstrate its potential for further developments by a case study which combines ATR-FT-IR spectroscopy with a recently published DFT...... a decreasing degree of polymerization to be a plausible cause, although others may interfere. Keywords: Cellulose; Mannan; FT-IR; DFT; Molecular modelling; Palm kernel...

Liquid natural gas. Japan

International Nuclear Information System (INIS)

Van Kooij, E.

1998-01-01

An overview is given of the technical know-how and expertise in Japan with respect to the supply, transport, storage and use of LNG. First the overall energy supply in Japan is outlined. Next, the reasons for the use of LNG as an energy source in Japan are discussed. As an example of a typical LNG-installation in Japan the construction of the Himeji Terminal of Osaka Gas Company is described. Finally, attention is paid to the world's largest and modern below-surface LNG-tanks (capacity of 200,000 m 3 ), installed at the Negishi Terminal of Tokyo Gas Company

Barbarian medicine in feudal Japan.

Science.gov (United States)

Fodstad, Harald; Hariz, Marwan I; Hirabayashi, Hidehiro; Ohye, Chihiro

2002-10-01

THE FIRST EUROPEANS to discover Japan were Portuguese traders who arrived in 1542. Fifteen years later, the Portuguese Jesuit priest and surgeon Luis De Almeida (1525-1583) founded the first Western hospital in Japan, for the care of lepers, syphilitics, and orphans. Because the hospital had a negative influence on the spread of Christianity, the Jesuits closed it in 1586. During the Tokugawa Shogunate (1600-1868), when Japan was secluded from the rest of the world, the only foreign physicians allowed to enter Japan were those employed by the Dutch factory at Dejima in Nagasaki. Only four of those physicians left behind seeds for the foundation of Western medicine in Japan, namely Caspar Schambergen, who founded a Japanese school of surgery in 1650; Engelbert Kämpfer, who visited Japan in 1691 to 1692; Carl Peter Thunberg, who botanically explored Japan in 1775 to 1776; and Philipp Franz Balthasar von Siebold, who practiced medicine in Nagasaki in 1823 to 1829 and 1859 to 1861. On the whole, Western medicine and surgery never established a real foothold in Japan until the fall of the shogunate and the restoration of the emperor in 1868.

Usage of FTIR-ATR as Non-Destructive Analysis of Selected Toxic Dyes

Directory of Open Access Journals (Sweden)

Bartošová Alica

2017-06-01

Full Text Available The degradation of the environment which is due to the discharge of polluting wastewater from industrial sources poses a real problem in several countries. Textile industries use large volumes of water in their operations, discharging thus large volume of wastewater into the environment, most of which is untreated. The wastewater contains a variety of chemicals from various stages of process operations, including desizing, scouring, bleaching and dyeing. The main purpose of this paper is to introduce Infrared Spectrometry with Fourier transformation as a non-destructive method for study, identifation and rapid determination of selected representatives of cationic (Methylene Blue, azo (Congo Red, Eriochrome Black T and nitroso (Naphthol Green B dyes. In conjunction with the ATR technique, FTIR offers a reliable detection method of dyes without extraction by other dangerous substances.

Convolutional neural network using generated data for SAR ATR with limited samples

Science.gov (United States)

Cong, Longjian; Gao, Lei; Zhang, Hui; Sun, Peng

2018-03-01

Being able to adapt all weather at all times, it has been a hot research topic that using Synthetic Aperture Radar(SAR) for remote sensing. Despite all the well-known advantages of SAR, it is hard to extract features because of its unique imaging methodology, and this challenge attracts the research interest of traditional Automatic Target Recognition(ATR) methods. With the development of deep learning technologies, convolutional neural networks(CNNs) give us another way out to detect and recognize targets, when a huge number of samples are available, but this premise is often not hold, when it comes to monitoring a specific type of ships. In this paper, we propose a method to enhance the performance of Faster R-CNN with limited samples to detect and recognize ships in SAR images.

Sensing cocaine in saliva with attenuated total reflection infrared (ATR-IR) spectroscopy combined with a one-step extraction method

Science.gov (United States)

Hans, Kerstin M.-C.; Gianella, Michele; Sigrist, Markus W.

2012-03-01

On-site drug tests have gained importance, e.g., for protecting the society from impaired drivers. Since today's drug tests are majorly only positive/negative, there is a great need for a reliable, portable and preferentially quantitative drug test. In the project IrSens we aim to bridge this gap with the development of an optical sensor platform based on infrared spectroscopy and focus on cocaine detection in saliva. We combine a one-step extraction method, a sample drying technique and infrared attenuated total reflection (ATR) spectroscopy. As a first step we have developed an extraction technique that allows us to extract cocaine from saliva to an almost infrared-transparent solvent and to record ATR spectra with a commercially available Fourier Transform-infrared spectrometer. To the best of our knowledge this is the first time that such a simple and easy-to-use one-step extraction method is used to transfer cocaine from saliva into an organic solvent and detect it quantitatively. With this new method we are able to reach a current limit of detection around 10 μg/ml. This new extraction method could also be applied to waste water monitoring and controlling caffeine content in beverages.

Japan and the global environment

International Nuclear Information System (INIS)

Miller, A.S.; Moore, C.

1993-01-01

In many areas, the word most often used to describe Japanese policy is open-quotes enigma.close quotes In some ways, Japan's record on environmental policy also has elements of mystery and contradiction. On the one hand, Japan's history and culture often are associated with a reverence for nature. Indeed, Japan does lead the world in certain environmental areas, such as reduction of conventional air pollutants and compensation of air pollution victims. On the other hand, Japan has been widely criticized for its poor record in preserving its domestic environment, contribution to tropical deforestation, and unwillingness to protect endangered species. Today, the international community clamors for Japan to take its share of responsibility, as an economic superpower, for the global environment. To secure its place in world affairs, Japan slowly has begun to respond to this pressure on issues ranging from ivory importation to reduction of CFC emissions. There is some hope that the government's particular willingness to address global warming may be a sign of significant changes to come in Japanese environmental policy. However, international pressure remains on the many environmental issues Japan has yet to resolve, including tropical deforestation and financing of Third World development projects that harm the environment. Japan's environmental policy is most effective when government and industry cooperate to find technical solutions to environmental problems. Although in recent years Japan's energy consumption has risen sharply, the Japanese have developed numerous technologies to reduce pollution and increase economic growth by improving energy efficiency. It is in these technologies that Japan has made the greatest strides and has the most to offer the global environment

Attenuated Total Reflection Fourier Transform Infrared (ATR FT-IR) Spectroscopy as an Analytical Method to Investigate the Secondary Structure of a Model Protein Embedded in Solid Lipid Matrices.

Science.gov (United States)

Zeeshan, Farrukh; Tabbassum, Misbah; Jorgensen, Lene; Medlicott, Natalie J

2018-02-01

Protein drugs may encounter conformational perturbations during the formulation processing of lipid-based solid dosage forms. In aqueous protein solutions, attenuated total reflection Fourier transform infrared (ATR FT-IR) spectroscopy can investigate these conformational changes following the subtraction of spectral interference of solvent with protein amide I bands. However, in solid dosage forms, the possible spectral contribution of lipid carriers to protein amide I band may be an obstacle to determine conformational alterations. The objective of this study was to develop an ATR FT-IR spectroscopic method for the analysis of protein secondary structure embedded in solid lipid matrices. Bovine serum albumin (BSA) was chosen as a model protein, while Precirol AT05 (glycerol palmitostearate, melting point 58 ℃) was employed as the model lipid matrix. Bovine serum albumin was incorporated into lipid using physical mixing, melting and mixing, or wet granulation mixing methods. Attenuated total reflection FT-IR spectroscopy and size exclusion chromatography (SEC) were performed for the analysis of BSA secondary structure and its dissolution in aqueous media, respectively. The results showed significant interference of Precirol ATO5 with BSA amide I band which was subtracted up to 90% w/w lipid content to analyze BSA secondary structure. In addition, ATR FT-IR spectroscopy also detected thermally denatured BSA solid alone and in the presence of lipid matrix indicating its suitability for the detection of denatured protein solids in lipid matrices. Despite being in the solid state, conformational changes occurred to BSA upon incorporation into solid lipid matrices. However, the extent of these conformational alterations was found to be dependent on the mixing method employed as indicated by area overlap calculations. For instance, the melting and mixing method imparted negligible effect on BSA secondary structure, whereas the wet granulation mixing method promoted

Uso de plasma rico en plaquetas autólogo en el tratamiento quirúrgico de pseudoartrosis atrófica de fémur y tibia

Directory of Open Access Journals (Sweden)

Julio C Vejarano-Solano

2013-04-01

Full Text Available Objetivo: Describir y evaluar los resultados del tratamiento quirúrgico de pseudoartrosis atrófica de fémur o tibia mediante el uso de plasma rico en plaquetas (PRP autólogo. Material y métodos: Estudio descriptivo longitudinal de 20 casos (19 pacientes de pseudoartrosis atrófica de fémur o tibia tratados quirúrgicamente con PRP autólogo en el Hospital Nacional Cayetano Heredia de Lima, desde enero del 2008 hasta enero del 2012. El PRP fue preparado en el banco de sangre del hospital a partir de una unidad de sangre autóloga. Se evaluó el tiempo de consolidación ósea mediante radiografías seriadas y se registraron las complicaciones. Resultados: Se incluyeron 12 varones y 7 mujeres, con edad promedio de 33,8 años (15 a 67 años. Se evidenció consolidación en el 95% de los pacientes, el tiempo promedio para la consolidación fue 25 semanas. Se observó un caso de persistencia de infección con cultivo positivo a E. coli y un caso de refractura. Conclusiones: El PRP autólogo tiene un efecto beneficioso en la reparación del tejido óseo en casos de pseudoartrosis atrófica.

Structural dependence of the efficiency of functionalization of silica-coated FeOx magnetic nanoparticles studied by ATR-IR

International Nuclear Information System (INIS)

Vargas, Angelo; Shnitko, Ivan; Teleki, Alexandra; Weyeneth, Stephen; Pratsinis, Sotiris E.; Baiker, Alfons

2011-01-01

The efficiency of propylamino functionalization of magnetic silica-coated FeO x nanoparticles prepared by different methods, including coprecipitation and flame aerosol synthesis, has been evaluated by attenuated total reflection infrared spectroscopy (ATR-IR) combined with a specific surface reaction, thus revealing the availability of the grafted functional groups. Large differences in the population of reactive groups were observed for the investigated materials, underlining the tight relation between the structure of nanoparticles and their suitability for organic functionalization. The materials possessed different core structure, surface area, and porosity, as evidenced by transmission electron microscopy and nitrogen adsorption-desorption isotherms. Grafting of aminopropyl groups using a standard procedure based on reaction with (3-aminopropyl)trimethoxysilane as source of the propylamino groups was performed, followed by classical dry analysis methods to determine the specific concentration of the organic functional groups (in mmol g -1 of material). ATR-IR spectroscopy in a specially constructed reactor cell was applied as wet methodology to determine the chemically available amount of such functional groups, showing that the materials possess largely different loading capacity, with a variability of up to 70% in the chemical availability of the organic functional group. The amount of (3-aminopropyl)trimethoxysilane used for functionalization was optimized, thus reaching a saturation limit characteristic of the material.

In-situ monitoring of blood glucose level for dialysis machine by AAA-battery-size ATR Fourier spectroscopy

Science.gov (United States)

Hosono, Satsuki; Sato, Shun; Ishida, Akane; Suzuki, Yo; Inohara, Daichi; Nogo, Kosuke; Abeygunawardhana, Pradeep K.; Suzuki, Satoru; Nishiyama, Akira; Wada, Kenji; Ishimaru, Ichiro

2015-07-01

For blood glucose level measurement of dialysis machines, we proposed AAA-battery-size ATR (Attenuated total reflection) Fourier spectroscopy in middle infrared light region. The proposed one-shot Fourier spectroscopic imaging is a near-common path and spatial phase-shift interferometer with high time resolution. Because numerous number of spectral data that is 60 (= camera frame rare e.g. 60[Hz]) multiplied by pixel number could be obtained in 1[sec.], statistical-averaging improvement realize high-accurate spectral measurement. We evaluated the quantitative accuracy of our proposed method for measuring glucose concentration in near-infrared light region with liquid cells. We confirmed that absorbance at 1600[nm] had high correlations with glucose concentrations (correlation coefficient: 0.92). But to measure whole-blood, complex light phenomenon caused from red blood cells, that is scattering and multiple reflection or so, deteriorate spectral data. Thus, we also proposed the ultrasound-assisted spectroscopic imaging that traps particles at standing-wave node. Thus, if ATR prism is oscillated mechanically, anti-node area is generated around evanescent light field on prism surface. By elimination complex light phenomenon of red blood cells, glucose concentration in whole-blood will be quantify with high accuracy. In this report, we successfully trapped red blood cells in normal saline solution with ultrasonic standing wave (frequency: 2[MHz]).

Japan`s civil use of foreign military plutonium

Energy Technology Data Exchange (ETDEWEB)

Suzuki, A. [Tokyo Univ. (Japan). Dept. of Quantum Engineering and Systems Sciences

1995-12-31

This paper is intended to propose one of the MOX options of international cooperation for safer and more secured management of excess military plutonium. The proposal was made with special reference to the Japanese public`s view. Owing to the domestic plutonium shortage anticipated soon after the 200 in Japan, some specific reactors will be available to get rid of foreign excess weapon plutonium. According to the Japan AEC`s new long-term programme, the shortage will be approximately 0.5 tonne of plutonium per annum, which is a sort of the least amount that Japan can buy from a certain external source. With international requests for a more positive Japanese contribution, however, the amount of Japanese purchase would be increased. It follows from the preliminary estimate shown in this paper that roughly 2 tonnes of plutonium can be burned annually in the reactors without any major modifications concerning safe reactor operation. (author) 10 refs.

Dynamic dependence on ATR and ATM for double-strand break repair in human embryonic stem cells and neural descendants.

Directory of Open Access Journals (Sweden)

Bret R Adams

2010-04-01

Full Text Available The DNA double-strand break (DSB is the most toxic form of DNA damage. Studies aimed at characterizing DNA repair during development suggest that homologous recombination repair (HRR is more critical in pluripotent cells compared to differentiated somatic cells in which nonhomologous end joining (NHEJ is dominant. We have characterized the DNA damage response (DDR and quality of DNA double-strand break (DSB repair in human embryonic stem cells (hESCs, and in vitro-derived neural cells. Resolution of ionizing radiation-induced foci (IRIF was used as a surrogate for DSB repair. The resolution of gamma-H2AX foci occurred at a slower rate in hESCs compared to neural progenitors (NPs and astrocytes perhaps reflective of more complex DSB repair in hESCs. In addition, the resolution of RAD51 foci, indicative of active homologous recombination repair (HRR, showed that hESCs as well as NPs have high capacity for HRR, whereas astrocytes do not. Importantly, the ATM kinase was shown to be critical for foci formation in astrocytes, but not in hESCs, suggesting that the DDR is different in these cells. Blocking the ATM kinase in astrocytes not only prevented the formation but also completely disassembled preformed repair foci. The ability of hESCs to form IRIF was abrogated with caffeine and siRNAs targeted against ATR, implicating that hESCs rely on ATR, rather than ATM for regulating DSB repair. This relationship dynamically changed as cells differentiated. Interestingly, while the inhibition of the DNA-PKcs kinase (and presumably non-homologous endjoining [NHEJ] in astrocytes slowed IRIF resolution it did not in hESCs, suggesting that repair in hESCs does not utilize DNA-PKcs. Altogether, our results show that hESCs have efficient DSB repair that is largely ATR-dependent HRR, whereas astrocytes critically depend on ATM for NHEJ, which, in part, is DNA-PKcs-independent.

Nuclear power development in Japan

International Nuclear Information System (INIS)

Sugawara, A.

1994-01-01

Energy situation in Japan and Japan's strategy for stable supply of energy are discussed. Benefits of nuclear power in comparison with other energy sources is considered. History of nuclear power development in Japan, modern status and future trends are described. 6 figs

Industrial lasers in Japan

Science.gov (United States)

Karube, Norio

1991-03-01

I am to report on some aspects of industrial lasers in Japan. Mostly centering on the market. In Japan, the history of laser developnent is rather profound. And long. Ever since the first invention of the laser in this country in 1960. This is partly because of the fact that in Japan the spectroscopic studies of the ruby was very popular in the late 1950's. Ever since niost of the work has been done in the research laboratories of the industry, not in the universities or not in the governmental laboratories. And since that time our first activity was mainly centering on the basic research, but after that time we have the evolution of the technology. One of the features in Japan is that the activity of developement and research of laser technology from the very basic phase up to the present commercialization has been done by the same group of people, including ine. We had a national project which ended about six years ago which was sponsored by MITI. MITI is Ministry of International Trade and Industry in Japan. And because of this national project, the effect of this project had a very enlightening effect in Japan. And after that our Japanese laser market became very flourishing.

Institutionalisation of Japan Identity Construction Policy

Directory of Open Access Journals (Sweden)

Zadvornaya Elena S.

2016-09-01

Full Text Available The article is devoted to the development of the modern politics of identity construction in Japan, which actively refers to the practice of designing self-image in international relations. This trend dates back to the 19th century, when there was the end of Sakoku (Japan’s policy of isolation. It is now possible to talk about the institutionalization of the identity construction policy to organized structures and regulations. Enhanced efforts in the field of Japanese traditional and popular culture, education and creative content has led to a number of institutions appearance (like Japan Foundation Fund, Japan Creative Agency, Japan Culture Fund, Agency for Cultural Affairs, Cool Japan Fund, Japan brand Fund and changing idea about the role of culture in foreign policy realization (it is fixed the documents of the Japanese Ministry of Foreign Affairs, the Ministry of Land, Infrastructure, Transport and Tourism, the Ministry of Education, Culture, Sports, Science and Technology, the Ministry of Economy, Trade and Industry, as well as a number of projects (Cool Japan, Visit Japan, Japan Culture Power, Japan Manga Awards, Kawaii Ambassadors, Cosplay International Fest and etc.. These efforts are aimed at forming Japan identity abroad to solve a number of foreign policy challenges of the future and the development of economic cooperation. The Japanese government nearest plans is to increase funding in order to create a positive image of Japan in the region of East Asia. All of these allows us to speak about the policy of the Japanese construction of identity as an institutionalized process in which there was clearance of organizations and regulatory activities.

Bacterial and abiotic decay in waterlogged archaeological Picea abies (L.) Karst studied by confocal Raman imaging and ATR-FTIR spectroscopy

DEFF Research Database (Denmark)

Pedersen, Nanna Bjerregaard; Gierlinger, Notburga; Thygesen, Lisbeth Garbrecht

2015-01-01

Waterlogged archaeological Norway spruce [Picea abies (L.) Karst] poles were studied by means of confocal Raman imaging (CRI) and attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR) analysis to determine lignin and polysaccharide composition and distribution in the cell......, and minor oxidation of the lignin polymer compared to recent reference material. This is evidence for abiotic decay in the course of waterlogging....

ATR technique, an appropriate method for determining the degree of conversion in dental giomers

International Nuclear Information System (INIS)

Prejmerean, Cristina; Prodan, Doina; Vlassa, Mihaela; Prejmerean, Vasile; Cuc, Stanca; Moldovan, Marioara; Streza, Mihaela; Buruiana, Tinca; Colceriu, Loredana

2016-01-01

Dental light-curing giomers were developed to combine the favourable properties of diacrylic resin composites (DRCs) and glass-ionomer cements (GICs) in a single material and to eliminate their inherent drawbacks. Giomers are characterized by their aesthetic appearance, high mechanical properties, adhesion to dental tissues as well as fluoride release and recharge abilities. The qualities of the giomers are greatly influenced by the level of conversion of the component resins. Infrared spectroscopy is one of the most largely used techniques for the determination of the degree of conversion in resin-based dental materials. However different results were obtained due to the performances of the used methods. The present work presents the determination of conversion degree in a series of dental copolymers and their corresponding giomers using transmission Fourier transform infrared spectroscopy (FTIR) and an attenuated total reflection technique (ATR) technique, respectively, the main aim being the study of the influence of the materials composition and of the light curing modes upon the achieved conversion in the cured giomers. Beautifil II commercial giomer was used as a control. A halogen lamp and a diode-blue LED lamp were used for the curing of the materials. The results showed that the composition of the resins greatly influenced the conversion. The highest conversions (up to 79%) were obtained in the case of the experimental giomers which contained the experimental Bis-GMA urethane analogue, followed by the Beautifil II giomer (61%) and experimental giomers based on commercial Bis-GMA (up to 50%), respectively. The resins light-cured by using the diode-blue LED lamp presented slightly higher conversions than the resins cured by halogen lamp. The study demonstrates the possibility to evaluate easily and reproducibly the conversion in light-curing composite materials with complex chemical composition and structure, particularly in the case of giomers by using the

Japan and LPG

International Nuclear Information System (INIS)

Anon.

1993-01-01

About 20 million metric tonnes of Liquefied Petroleum Gases (LPG) are consumed each year in Japan, of which 14,5 million metric tonnes are imported from Saudi Arabia and United Arab Emirates. This paper presents LPG industry in Japan and gives informations on the present status: supply and demand, imports, market, distribution

Nuclear energy in Japan

International Nuclear Information System (INIS)

Guillemard, B.

1978-01-01

After having described the nuclear partners in Japan, the author analyzes the main aspects of Japan's nuclear energy: nuclear power plants construction program; developping of light water reactors; fuel cycle politics [fr

Globalization in Japan

DEFF Research Database (Denmark)

Roesgaard, Marie Højlund

2014-01-01

Abstract for Nichibunken Copenhagen Symposium August 2012 Globalization in Japan – the case of moral education. 日本とグローバル化　－　道徳教育の件 Marie H. Roesgaard, Department of Cross-Cultural and Regional Studies, University of Copenhagen. This paper attempts to trace the history of global influence on Japan......Abstract for Nichibunken Copenhagen Symposium August 2012 Globalization in Japan – the case of moral education. 日本とグローバル化　－　道徳教育の件 Marie H. Roesgaard, Department of Cross-Cultural and Regional Studies, University of Copenhagen. This paper attempts to trace the history of global influence...... adjusting to those of the global currents that cannot be ignored. Further, I would suggest that global, or at least Western, influence is not a new thing in regard to moral education in Japan. The paper will provide an historical overview of the development of moral education since Meiji times and focus...

Determination of drug content in semisolid formulations by non-invasive spectroscopic methods: FTIR - ATR, - PAS, - Raman and PDS

International Nuclear Information System (INIS)

Gotter, B; Hein, J; Neubert, R H H; Faubel, W; Heissler, St

2010-01-01

This study elucidates the potential use of photothermal deflection spectroscopy (PDS), FTIR photoacoustic (FTIR-PAS), FT Raman, and FTIR-attenuated total reflection (FTIR-ATR) spectroscopy as analytical tools for investigating the drug content in semisolid formulations. Regarding the analytical parameters, this study demonstrates the photothermal beam deflection to be definitely comparable to well established spectroscopic methods for this purpose. The correlation coefficients range from 0.990 to 0.999. Likewise, repeatability and limit of detection are comparable.

Microelectronics in Japan

Science.gov (United States)

Boulton, William R.

1995-02-01

The purpose of this JTEC study is to evaluate Japan's electronic manufacturing and packaging capabilities within the context of global economic competition. To carry out this study, the JTEC panel evaluated the framework of the Japanese consumer electronics industry and various technological and organizational factors that are likely to determine who will win and lose in the marketplace. This study begins with a brief overview of the electronics industry, especially as it operates in Japan today. Succeeding chapters examine the electronics infrastructure in Japan and take an in-depth look at the central issues of product development in order to identify those parameters that will determine future directions for electronic packaging technologies.

Rapid detection of NBOME's and other NPS on blotter papers by direct ATR-FTIR spectrometry.

Science.gov (United States)

Coelho Neto, José

2015-07-01

Blotter paper is among the most common forms of consumption of new psychotropic substances (NPS), formerly referred as designer drugs. In many cases, users are misled to believe they are taking LSD when, in fact, they are taking newer and less known drugs like the NBOMEs or other substituted phenethylamines. We report our findings in quick testing of blotter papers for illicit substances like NBOMEs and other NPS by taking ATR-FTIR spectra directly from blotters seized on the streets, without any sample preparation. Both sides (front and back) of each blotter were tested. Collected data were analyzed by single- and multi-component spectral matching and submitted to chemometric discriminant analysis. Our results showed that, on 66.7% of the cases analyzed, seized blotters contained one or more types of NBOMEs, confirming the growing presence of this novel substances on the market. Matching IR signals were detected on both or just one side of the blotters and showed variable strength. Although no quantitative analysis was made, detection of these substances by the proposed approach serves as indication of variable and possibly higher dosages per blotter when compared to LSD, which showed to be below the detection limit of the applied method. Blotters containing a mescaline-like compound, later confirmed by GC-MS and LC-MS to be MAL (methallylescaline), a substance very similar to mescaline, were detected among the samples tested. Validity of direct ATR-FTIR testing was confirmed by checking the obtained results against independent GC-MS or LC-MS results for the same cases/samples. Copyright © 2015 Elsevier Ireland Ltd. All rights reserved.

Formation of carboxy- and amide-terminated alkyl monolayers on silicon(111) investigated by ATR-FTIR, XPS, and X-ray scattering: Construction of photoswitchable surfaces

DEFF Research Database (Denmark)

Rück-Braun, Karola; Petersen, Michael Åxman; Michalik, Fabian

2013-01-01

-FTIR and XPS studies of the fulgimide samples revealed closely covered amide-terminated SAMs. Reversible photoswitching of the headgroup was read out by applying XPS, ATR-FTIR, and difference absorption spectra in the mid-IR. In XPS, we observed a reversible breathing of the amide/imide C1s and N1s signals......We have prepared high-quality, densely packed, self-assembled monolayers (SAMs) of carboxy-terminated alkyl chains on Si(111). The samples were made by thermal grafting of methyl undec-10-enoate under an inert atmosphere and subsequent cleavage of the ester functionality to disclose the carboxylic...... zigzag-like substitution pattern for the ester- and carboxy-terminated monolayer. Hydrolysis of the remaining H-Si(111) bonds at the surface furnished HO-Si(111) groups according to XPS and attenuated total reflection Fourier-transform infrared spectroscopy (ATR-FTIR) studies. The amide-terminated alkyl...

Correlation Study of PVDF Membrane Morphology with Protein Adsorption: Quantitative Analysis by FTIR/ATR Technique

Science.gov (United States)

Ideris, N.; Ahmad, A. L.; Ooi, B. S.; Low, S. C.

2018-05-01

Microporous PVDF membranes were used as protein capture matrices in immunoassays. Because the most common labels in immunoassays were detected based on the colour change, an understanding of how protein concentration varies on different PVDF surfaces was needed. Herein, the correlation between the membrane pore size and protein adsorption was systematically investigated. Five different PVDF membrane morphologies were prepared and FTIR/ATR was employed to accurately quantify the surface protein concentration on membranes with small pore sizes. SigmaPlot® was used to find a suitable curve fit for protein adsorption and membrane pore size, with a high correlation coefficient, R2, of 0.9971.

Sensing molecular properties by ATR-SPP Raman spectroscopy on electrochemically structured sensor chips

International Nuclear Information System (INIS)

Zerulla, D.; Isfort, G.; Koelbach, M.; Otto, A.; Schierbaum, K.

2003-01-01

The use of electrochemically structured Al surfaces as sensor arrays for combinatorial chemistry and its detection via microscopic laser techniques from very small volumes has been explored. The methodology is based on three different techniques which will be discussed separately: firstly, attenuated total reflection (ATR) is used in connection with surface-plasmon-polariton (SPP) excitation. A thin Al layer, evaporated on sapphire or quartz and covered with a naturally grown oxide layer, provides an optimum enhancement and confinement of the electrical field close to the surface. This is revealed by calculations and experimental data. Secondly, a Raman microscope is applied, enabling chemical spot analysis in the visible and UV range with a lateral resolution close to the diffraction limit. Finally, its application to investigate electrochemically structured Al films is discussed

Effect of dissolved oxygen on two bacterial pathogens examined using ATR-FTIR spectroscopy, microelectrophoresis, and potentiometric titration.

Science.gov (United States)

Castro, Felipe D; Sedman, Jacqueline; Ismail, Ashraf A; Asadishad, Bahareh; Tufenkji, Nathalie

2010-06-01

The effects of dissolved oxygen tension during bacterial growth and acclimation on the cell surface properties and biochemical composition of the bacterial pathogens Escherichia coli O157:H7 and Yersinia enterocolitica are characterized. Three experimental techniques are used in an effort to understand the influence of bacterial growth and acclimation conditions on cell surface charge and the composition of the bacterial cell: (i) electrophoretic mobility measurements; (ii) potentiometric titration; and (iii) ATR-FTIR spectroscopy. Potentiometric titration data analyzed using chemical speciation software are related to measured electrophoretic mobilities at the pH of interest. Titration of bacterial cells is used to identify the major proton-active functional groups and the overall concentration of these cell surface ligands at the cell membrane. Analysis of titration data shows notable differences between strains and conditions, confirming the appropriateness of this tool for an overall charge characterization. ATR-FTIR spectroscopy of whole cells is used to further characterize the bacterial biochemical composition and macromolecular structures that might be involved in the development of the net surficial charge of the organisms examined. The evaluation of the integrated intensities of HPO(2)(-) and carbohydrate absorption bands in the IR spectra reveals clear differences between growth protocols. Taken together, the three techniques seem to indicate that the dissolved oxygen tension during cell growth or acclimation can noticeably influence the expression of cell surface molecules and the measurable cell surface charge, though in a strain-dependent fashion.

ATR-p53 restricts homologous recombination in response to replicative stress but does not limit DNA interstrand crosslink repair in lung cancer cells.

Directory of Open Access Journals (Sweden)

Bianca M Sirbu

Full Text Available Homologous recombination (HR is required for the restart of collapsed DNA replication forks and error-free repair of DNA double-strand breaks (DSB. However, unscheduled or hyperactive HR may lead to genomic instability and promote cancer development. The cellular factors that restrict HR processes in mammalian cells are only beginning to be elucidated. The tumor suppressor p53 has been implicated in the suppression of HR though it has remained unclear why p53, as the guardian of the genome, would impair an error-free repair process. Here, we show for the first time that p53 downregulates foci formation of the RAD51 recombinase in response to replicative stress in H1299 lung cancer cells in a manner that is independent of its role as a transcription factor. We find that this downregulation of HR is not only completely dependent on the binding site of p53 with replication protein A but also the ATR/ATM serine 15 phosphorylation site. Genetic analysis suggests that ATR but not ATM kinase modulates p53's function in HR. The suppression of HR by p53 can be bypassed under experimental conditions that cause DSB either directly or indirectly, in line with p53's role as a guardian of the genome. As a result, transactivation-inactive p53 does not compromise the resistance of H1299 cells to the interstrand crosslinking agent mitomycin C. Altogether, our data support a model in which p53 plays an anti-recombinogenic role in the ATR-dependent mammalian replication checkpoint but does not impair a cell's ability to use HR for the removal of DSB induced by cytotoxic agents.

Japan steel mill perspective

Energy Technology Data Exchange (ETDEWEB)

Murase, K. [Kobe Steel Ltd., Tokyo (Japan)

2004-07-01

The international and Japan's steel industry, the coking coal market, and Japan's expectations from Canada's coal industry are discussed. Japan's steel mills are operating at full capacity. Crude steel production for the first half of 2004 was 55.8 million tons. The steel mills are profitable, but costs are high, and there are difficulties with procuring raw materials. Japan is trying to enhance the quality of coke, in order to achieve higher productivity in the production of pig iron. Economic growth is rising disproportionately in the BRICs (Brazil, Russia, India, and China), with a large increase in coking coal demand from China. On the supply side, there are several projects underway in Australia and Canada to increase production. These include new developments by Elk Valley Coal Corporation, Grande Cache Coal, Western Canadian Coal, and Northern Energy and Mining in Canada. The Elga Mine in the far eastern part of Russia is under development. But the market is expected to remain tight for some time. Japan envisions Canadian coal producers will provide a stable coal supply, expansion of production and infrastructure capabilities, and stabilization of price. 16 slides/overheads are included.

Usage of FTIR-ATR as Non-Destructive Analysis of Selected Toxic Dyes

Science.gov (United States)

Bartošová, Alica; Blinová, Lenka; Sirotiak, Maroš; Michalíková, Anna

2017-06-01

The degradation of the environment which is due to the discharge of polluting wastewater from industrial sources poses a real problem in several countries. Textile industries use large volumes of water in their operations, discharging thus large volume of wastewater into the environment, most of which is untreated. The wastewater contains a variety of chemicals from various stages of process operations, including desizing, scouring, bleaching and dyeing. The main purpose of this paper is to introduce Infrared Spectrometry with Fourier transformation as a non-destructive method for study, identifation and rapid determination of selected representatives of cationic (Methylene Blue), azo (Congo Red, Eriochrome Black T) and nitroso (Naphthol Green B) dyes. In conjunction with the ATR technique, FTIR offers a reliable detection method of dyes without extraction by other dangerous substances. Spectral interpretation of dye spectra revealed valuable information about the identification and characterization of each group of dyes.

The Mismatch-Binding Factor MutSβ Can Mediate ATR Activation in Response to DNA Double-Strand Breaks

Czech Academy of Sciences Publication Activity Database

Burdová, Kamila; Mihaljevic, B.; Sturzenegger, A.; Chappidi, N.; Janščák, Pavel

2015-01-01

Roč. 59, č. 4 (2015), s. 603-614 ISSN 1097-2765 R&D Projects: GA ČR GAP305/10/0281; GA ČR(CZ) GA14-05743S Grant - others:Oncosuisse(CH) KLS-02344-02-2009; Swiss National Science Foundation(CH) 31003A_146206; Novartis Foundation for Medical and Biological Research(CH) 11A16 Institutional support: RVO:68378050 Keywords : Ataxia telangiectasia-mutated and Rad3-related (ATR) protein kinase * DNA-damage response * DNA Double-Strand Breaks Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 13.958, year: 2015

[Building epilepsy care network in Japan].

Science.gov (United States)

Otsuki, Taisuke

2012-01-01

Number of epilepsy patient in Japan officially surveyed by our government in 2008 is 219,000, which is only 0.17% of the total population and less than one third of the prevalence rate reported in Western countries. Number of epilepsy surgery per year in Japan is also low and less than half of other countries such as US, UK and Korea. These numbers may suggest that epilepsy care in Japan is not sufficient to cover all hidden medical needs of people with epilepsy at present. To solve this issue, our research group funded by the government have started to build an epilepsy care network among primary care physicians, secondary care neurology specialists and tertiary care epilepsy centers by utilizing a web site: Epilepsy Care Network-Japan (http://www.ecn-japan.com/) from July 2012. We are also proposing an epilepsy care algorithm suitable for our complex medical community consisted with various neurology specialists such as pediatric and adult neurologists, neurosurgeons and psychiatrists. Building Epilepsy Care Network in Japan may facilitate better medical and social support for people with epilepsy in Japan.

Positioning Indian Emigration to Japan

DEFF Research Database (Denmark)

D'Costa, Anthony

2013-01-01

as other IT-strong developing countries, are to supply technical talent, whose availability in Japan is constrained by the secular demographic crisis and changing educational and occupational preferences. The challenges for India are the institutional barriers, in particular, Japanese business practices...... that act as significant barriers to the entry of foreign skilled professionals. The paper brings out the source and pattern of foreign professionals and students in Japan as a proxy for talent. Though India’s presence in Japan is currently limited, its share of technical professionals to the total number....... For Japan, it means access to technical professionals and managed interfacing with the global economy....

78 FR 78338 - Japan-U.S. Decommissioning and Remediation Fukushima Recovery Forum Tokyo, Japan February 18-19...

Science.gov (United States)

2013-12-26

... DEPARTMENT OF COMMERCE International Trade Administration Japan-U.S. Decommissioning and Remediation Fukushima Recovery Forum Tokyo, Japan February 18-19, 2014 AGENCY: International Trade... Japan-United States Decommissioning and Remediation Fukushima Recovery Forum (``Fukushima Recovery Forum...

Measuring Heterogeneous Reaction Rates with ATR-FTIR Spectroscopy to Evaluate Chemical Fates in an Atmospheric Environment: A Physical Chemistry and Environmental Chemistry Laboratory Experiment

Science.gov (United States)

Roberts, Jason E.; Zeng, Guang; Maron, Marta K.; Mach, Mindy; Dwebi, Iman; Liu, Yong

2016-01-01

This paper reports an undergraduate laboratory experiment to measure heterogeneous liquid/gas reaction kinetics (ozone-oleic acid and ozone-phenothrin) using a flow reactor coupled to an attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectrometer. The experiment is specially designed for an upper-level undergraduate Physical…

Japan's nuclear juggernaut

International Nuclear Information System (INIS)

Richner, S.

1984-01-01

A summary of nuclear energy in Japan is presented. Nuclear energy provides 17% of Japan's electricity but could provide much more. 25 more reactors (to add to the existing 27) are planned by 1995. The cooperation between Japanese government and industry and the economic assistance in building new plants means that these are not likely to be cancelled. Public acceptance of new plants has often been obtained by large cash employment inducements. Now, however, there is growing disillusionment when short-term well paid employment building the reactors is followed by unemployment when the reactor is in operation. Also there is a growing opposition to the storage of the nuclear waste. To maintain the growth of its nuclear industry Japan needs to export to provide a steady flow of big orders. (U.K.)

Spent fuel management in Japan

International Nuclear Information System (INIS)

Shirahashi, K.; Maeda, M.; Nakai, T.

1996-01-01

Japan has scarce energy resources and depends on foreign resources for 84% of its energy needs. Therefore, Japan has made efforts to utilize nuclear power as a key energy source since mid-1950's. Today, the nuclear energy produced from 49 nuclear power plants is responsible for about 31% of Japan's total electricity supply. The cumulative amount of spent fuel generated as of March 1995 was about 11,600 Mg U. Japan's policy of spent fuel management is to reprocess spent nuclear fuel and recycle recovered plutonium and uranium as nuclear fuel. The Tokai reprocessing plant continues stable operation keeping the annual treatment capacity or around 90 Mg U. A commercial reprocessing plant is under construction at Rokkasho, northern part of Japan. Although FBR is the principal reactor to use plutonium, LWR will be a major power source for some time and recycling of the fuel in LWRs will be prompted. (author). 3 figs

Characteristics of Wet Deposition in Japan

Science.gov (United States)

Iwasaki, A.; Arakaki, T.

2017-12-01

Acid deposition survey in Japan has started since 1991 by Japan Environmental Laboratories Association (JELA). The JELA has about 60 monitoring sites for wet deposition including remote, rural and urban area. The measured constituents of wet deposition are; precipitation, pH, electric conductivity, major Anions, and major Cations. From those data, we analyze spatial and temporal variations of wet deposition components in Japan. Among the 60 monitoring sites, 39 sampling sites were selected in this study, which have kept sampling continuously between 2003JFY and 2014JFY. All samples were collected by wet-only samplers. To analyze area characteristics, all the areas were divided into 6 regions; Northern part of Japan (NJ), Facing the Japan Sea (JS), Eastern part of Japan (EJ), Central part of Japan (CJ), Western part of Japan (WJ) and Southern West Islands (SW). NO3- and non-sea-salt-SO42- (nss-SO42-) are major components of rain acidification. Especially, between December and February (winter) the air mass from west affected the temporal variations of those acid components and the concentrations were higher in JS and WJ regions than those in other regions. Japanese ministry of the Environment reported that mixing ratio of NO2 in Japan has been less than 0.04ppm since 1976, and that of SO2 has been less than 0.02ppm since 1978. Their concentrations in Japan have remained flat or slowly decreased recently. However the temporal variations of NO3-/nss-SO42- ratio in winter in JS region were significantly increased on average at 2.2% y-1 from 2003JFY to 2014JFY. The results suggest that long-range transboundary air pollutants increased NO3- concentrations and NO3-/nss-SO42- ratio.

Nuclear power development in Japan

International Nuclear Information System (INIS)

Sugawara, A.

1994-01-01

The energy situation in Japan is briefly outlined. Vulnerability in energy structure of the country is shown by a comparison of primary energy supply patterns of Japan and Western countries. Japan's energy policy consists in reducing dependence on oil, promoting efficient use of energy and increasing use of non-fossil fuels. Nuclear power is a core of alternative energy for petroleum because of stable supply of nuclear fuel, low detrimental emissions and less dependence on the fuel. A short historical review of nuclear power development in Japan is presented. Some future issues as development of entire nuclear fuel cycle, social acceptance, reactor safety and nuclear power economics are also discussed. 6 figs. (R.T.)

Advances in food composition tables in Japan-Standard Tables Of Food Composition in Japan - 2015 - (Seventh Revised Edition).

Science.gov (United States)

Watanabe, Tomoko; Kawai, Ryoko

2018-01-01

The latest version of the Standard Tables of Food Composition in Japan-2015- comprises the main food composition table (Standard Tables of Food Composition in Japan-2015-[Seventh revised Edition)) and three supplementary books. The supplementary books are Standard Tables of Food Composition in Japan - 2015 - (Seventh Revised Edition) - Amino Acids -, Standard Tables of Food Composition in Japan - 2015 - (Seventh Revised Edition) - Fatty Acids - and Standard Tables of Food Composition in Japan - 2015 - (Seventh Revised Edition) - Available Carbohydrates, Polyols and Organic Acids-. We believe understanding these food composition tables can give greater insight into Japan's gastronomic culture and changes in eating habits. We expect them to play important roles as part of the East Asia food composition tables. Copyright © 2017. Published by Elsevier Ltd.

Japan's electronic packaging technologies

Science.gov (United States)

Tummala, Rao R.; Pecht, Michael

1995-02-01

The JTEC panel found Japan to have significant leadership over the United States in the strategic area of electronic packaging. Many technologies and products once considered the 'heart and soul' of U.S. industry have been lost over the past decades to Japan and other Asian countries. The loss of consumer electronics technologies and products is the most notable of these losses, because electronics is the United States' largest employment sector and is critical for growth businesses in consumer products, computers, automobiles, aerospace, and telecommunications. In the past there was a distinction between consumer and industrial product technologies. While Japan concentrated on the consumer market, the United States dominated the industrial sector. No such distinction is anticipated in the future; the consumer-oriented technologies Japan has dominated are expected to characterize both domains. The future of U.S. competitiveness will, therefore, depend on the ability of the United States to rebuild its technological capabilities in the area of portable electronic packaging.

Structural dependence of the efficiency of functionalization of silica-coated FeO{sub x} magnetic nanoparticles studied by ATR-IR

Energy Technology Data Exchange (ETDEWEB)

Vargas, Angelo; Shnitko, Ivan [Institute for Chemical and Bioengineering, Department of Chemistry and Applied Biosciences, ETH Zurich, Wolfgang Pauli Strasse 10, Hoenggerberg, HCI, 8093 Zurich (Switzerland); Teleki, Alexandra [Institute of Process Engineering, Department of Mechanical and Process Engineering, ETH Zurich, 8092 Zurich (Switzerland); Weyeneth, Stephen [Physik-Institut der Universitaet Zuerich, Winterthurerstrasse 190, 8057 Zuerich (Switzerland); Pratsinis, Sotiris E. [Institute of Process Engineering, Department of Mechanical and Process Engineering, ETH Zurich, 8092 Zurich (Switzerland); Baiker, Alfons, E-mail: baiker@chem.ethz.ch [Institute for Chemical and Bioengineering, Department of Chemistry and Applied Biosciences, ETH Zurich, Wolfgang Pauli Strasse 10, Hoenggerberg, HCI, 8093 Zurich (Switzerland)

2011-01-15

The efficiency of propylamino functionalization of magnetic silica-coated FeO{sub x} nanoparticles prepared by different methods, including coprecipitation and flame aerosol synthesis, has been evaluated by attenuated total reflection infrared spectroscopy (ATR-IR) combined with a specific surface reaction, thus revealing the availability of the grafted functional groups. Large differences in the population of reactive groups were observed for the investigated materials, underlining the tight relation between the structure of nanoparticles and their suitability for organic functionalization. The materials possessed different core structure, surface area, and porosity, as evidenced by transmission electron microscopy and nitrogen adsorption-desorption isotherms. Grafting of aminopropyl groups using a standard procedure based on reaction with (3-aminopropyl)trimethoxysilane as source of the propylamino groups was performed, followed by classical dry analysis methods to determine the specific concentration of the organic functional groups (in mmol g{sup -1} of material). ATR-IR spectroscopy in a specially constructed reactor cell was applied as wet methodology to determine the chemically available amount of such functional groups, showing that the materials possess largely different loading capacity, with a variability of up to 70% in the chemical availability of the organic functional group. The amount of (3-aminopropyl)trimethoxysilane used for functionalization was optimized, thus reaching a saturation limit characteristic of the material.

ATR-FTIR Spectroscopic Evidence for Biomolecular Phosphorus and Carboxyl Groups Facilitating Bacterial Adhesion to Iron Oxides

Science.gov (United States)

Parikh, Sanjai J.; Mukome, Fungai N.D.; Zhang, Xiaoming

2014-01-01

Attenuated total reflectance (ATR) Fourier transform infrared (FTIR) spectroscopy has been used to probe the binding of bacteria to hematite (α-Fe2O3) and goethite (α-FeOOH). In situ ATR-FTIR experiments with bacteria (Pseudomonas putida, P. aeruginosa, Escherichia coli), mixed amino acids, polypeptide extracts, deoxyribonucleic acid (DNA), and a suite of model compounds were conducted. These compounds represent carboxyl, catecholate, amide, and phosphate groups present in siderophores, amino acids, polysaccharides, phospholipids, and DNA. Due in part to the ubiquitous presence of carboxyl groups in biomolecules, numerous IR peaks corresponding to outer-sphere or unbound (1400 cm−1) and inner-sphere (1310-1320 cm−1) coordinated carboxyl groups are noted following reaction of bacteria and biomolecules with α-Fe2O3 and α-FeOOH. However, the data also reveal that the presence of low-level amounts (i.e., 0.45-0.79%) of biomolecular phosphorous groups result in strong IR bands at ~1043 cm−1, corresponding to inner-sphere Fe-O-P bonds, underscoring the importance of bacteria associated P-containing groups in biomolecule and cell adhesion. Spectral comparisons also reveal slightly greater P-O-Fe contributions for bacteria (Pseudomonad, E. coli) deposited on α-FeOOH, as compared to α-Fe2O3. This data demonstrates that slight differences in bacterial adhesion to Fe oxides can be attributed to bacterial species and Fe-oxide minerals. However, more importantly, the strong binding affinity of phosphate in all bacteria samples to both Fe-oxides results in the formation of inner-sphere Fe-O-P bonds, signifying the critical role of biomolecular P in the initiation of bacterial adhesion. PMID:24859052

ATR-FTIR spectroscopy and quantitative multivariate analysis of paints and coating materials

Science.gov (United States)

Hayes, Philippa Alice; Vahur, Signe; Leito, Ivo

2014-12-01

The applicability of ATR-FTIR spectroscopy with partial least squares (PLS) data analysis was evaluated for quantifying the components of mixtures of paint binding media and pigments, and alkyd resins. PLS methods were created using a number of standard mixtures. Validation and measurement uncertainty estimation was carried out. Binary, ternary and quaternary mixtures of several common binding media and pigments were quantified, with standard measurement uncertainties in most cases below 3 g/100 g. Classes of components - aromatic anhydrides and alcohols - used in alkyd resin synthesis were also successfully quantified, with standard uncertainties in the range of 2-3 g/100 g. This is a more demanding application because in alkyd resins aromatic anhydrides and alcohols have reacted to form a polyester, and are not present in their original forms. Once a PLS method has been calibrated, analysis time and cost are significantly reduced from typical quantitative methods such as GC/MS. This is beneficial in the case of routine analysis where the components are known.

Current Status of Doping in Japan Based on Japan Anti-Doping Disciplinary Panels of the Japan Anti-Doping Agency (JADA): A Suggestion on Anti-Doping Activities by Pharmacists in Japan.

Science.gov (United States)

Imanishi, Takashi; Kawabata, Takayoshi; Takayama, Akira

2017-01-01

In 2009, the Japan Anti-Doping Agency (JADA) established the "Sports Pharmacist Accreditation Program" to prevent doping in sports. Since then, anti-doping activities in Japan have been attracting attention. In this study, we investigated research about the current status of doping from 2007 to 2014 in Japan to make anti-doping activities more concrete, and we also discussed future anti-doping activities by pharmacists. In Japan, bodybuilding was the sporting event with the highest number and rate of doping from 2007 to 2014. Many of the positive doping cases were detected for class S1 (anabolic agents), S5 (diuretics and masking agents), and S6 (stimulants). Within class S1, supplements were the main cause of positive doping. Within class S5, medicines prescribed by medical doctors were the main cause of positive doping. Within class S6, non-prescription medicines (e.g., OTC) were the main cause of positive doping. When we looked at the global statistics on doping, many of the positive doping cases were detected for class S1. On comparing the Japanese statistics with the global statistics, the rate of positive doping caused by class S1 was significantly lower, but that caused by classes S5 and S6 was significantly higher in Japan than in the world. In conclusion, pharmacists in Japan should pay attention to class S1, S5, and S6 prohibited substances and to the sport events of bodybuilding. Based on this study, sports pharmacists as well as common pharmacists should suggest new anti-doping activities to prevent doping in the future.

Japan's advanced medicine.

Science.gov (United States)

Sho, Ri; Narimatsu, Hiroto; Murakami, Masayasu

2013-10-01

Like health care systems in other developed countries, Japan's health care system faces significant challenges due to aging of the population and economic stagnation. Advanced medicine (Senshin Iryou) is a unique system of medical care in Japan offering highly technology-driven medical care that is not covered by public health insurance. Advanced medicine has recently developed and expanded as part of health care reform. Will it work? To answer this question, we briefly trace the historical development of advanced medicine and describe the characteristics and current state of advanced medical care in Japan. We then offer our opinions on the future of advanced medicine with careful consideration of its pros and cons. We believe that developing advanced medicine is an attempt to bring health care reform in line rather than the goal of health care reform.

Development of refueling support system

International Nuclear Information System (INIS)

Nishimura, Hiroshi; Hayashi, Shoichi; Sano, Kazuya; Hochin, Koji; Iguchi, Yukihiro

1992-01-01

The refueling of Fugen Nuclear Power Station requires correct management of fuels, etc. And empirical knowledge is necessary for efficient planning and refueling. Fugen developed refueling support system and put it into practical operation. The system features a network of refueling equipment and AI rules aquired from operators knowledge. The system helps make an optimized plan, displays step-by-step guidance and prints out lists of fuel locations and movements. The system reduced the labor of the operators, optimized the management and improved the reliability of the refueling. (author)

A rapid ATR-FTIR spectroscopic method for detection of sibutramine adulteration in tea and coffee based on hierarchical cluster and principal component analyses.

Science.gov (United States)

Cebi, Nur; Yilmaz, Mustafa Tahsin; Sagdic, Osman

2017-08-15

Sibutramine may be illicitly included in herbal slimming foods and supplements marketed as "100% natural" to enhance weight loss. Considering public health and legal regulations, there is an urgent need for effective, rapid and reliable techniques to detect sibutramine in dietetic herbal foods, teas and dietary supplements. This research comprehensively explored, for the first time, detection of sibutramine in green tea, green coffee and mixed herbal tea using ATR-FTIR spectroscopic technique combined with chemometrics. Hierarchical cluster analysis and PCA principle component analysis techniques were employed in spectral range (2746-2656cm -1 ) for classification and discrimination through Euclidian distance and Ward's algorithm. Unadulterated and adulterated samples were classified and discriminated with respect to their sibutramine contents with perfect accuracy without any false prediction. The results suggest that existence of the active substance could be successfully determined at the levels in the range of 0.375-12mg in totally 1.75g of green tea, green coffee and mixed herbal tea by using FTIR-ATR technique combined with chemometrics. Copyright © 2017 Elsevier Ltd. All rights reserved.

Observations of surface-mediated reduction of Pu(VI) to Pu(IV) on hematite nanoparticles by ATR FT-IR

Energy Technology Data Exchange (ETDEWEB)

Emerson, Hilary P. [Florida International Univ., Applied Research Center, Miami, FL (United States); Powell, Brian A. [Clemson Univ., Dept. of Enviromental Engineering and Earth Sciences, Anderson, SC (United States)

2015-07-01

Previous studies have shown that mineral surfaces may facilitate the reduction of plutonium though the mechanisms of the reduction are still unknown. The objective of this study is to use batch sorption and attenuated total reflectance Fourier transform infrared spectroscopy experiments to observe the surface-mediated reduction of plutonium on hematite nanoparticles. These techniques allow for in situ measurement of reduction of plutonium with time and may lead to a better understanding of the mechanisms of surface mediated reduction of plutonium. For the first time, ATR FT-IR peaks for Pu(VI) sorbed to hematite are measured at ∝ 916 cm{sup -1}, respectively. The decrease in peak intensity with time provides a real-time, direct measurement of Pu(VI) reduction on the hematite surface. In this work pseudo first order rate constants estimated at the high loadings (22 mg{sub Pu}/g{sub hematite}, 1.34 x 10{sup -6} M{sub Pu}/m{sup 2}) for ATR FT-IR are approximately 10 x slower than at trace concentrations based on previous work. It is proposed that the reduced rate constant at higher Pu loadings occurs after the reduction capacity due to trace Fe(II) has been exhausted and is dependent on the oxidation of water and possibly electron shuttling based on the semiconducting nature of hematite. Therefore, the reduction rate at higher loadings is possibly due to the thermodynamic favorability of Pu(IV)-hydroxide complexes.

Detection of creatinine enriched on a surface imprinted polystyrene film using FT-ATR-IR.

Science.gov (United States)

Sreenivasan, K

2006-01-01

The surface of polystyrene (PS) was chemically modified by coating a thin layer of polyaniline (PANI) by oxidizing aniline using ammonium persulfate. Affinity sites for creatinine, a clinically relevant molecule, were created in the coated layer by adding creatinine as print molecules during the oxidation. The imprinted layer adsorbed creatinine was compared to non-imprinted surface reflecting the creation of creatinine-specific sites on the surface. The equilibrium was attained rapidly, indicating that a material of this kind is suitable for sensing applications. The adsorbed creatinine on the surface was detected using the technique of Fourier transform attenuated total internal reflection infra red spectroscopy (FT-ATR-IR). The results show that molecularly imprinted surface can enrich molecules of interest and the enriched molecules can be detected using FT-IR.

Characterisation of 17th-18th centuries damask and gilt leathers by ATR-FTIR

Directory of Open Access Journals (Sweden)

Lina Falcão

2018-01-01

Full Text Available In this paper it is presented the characterisation of tannins used in the vegetable tanning of two types of European historic and decorative leathers from Portuguese collections, damask leathers and gilt leathers, dated from the 17th and 18th centuries. Extracts prepared from collected leather fibres were analysed by attenuated total reflectance – Fourier transform infrared (ATR-FTIR spectroscopy. Results were compared with data collected from different reference vegetable tanning materials which, according to historic and technical literature, were used in Europe during the Modern Age. This study allowed, in most cases, the characterisation of the tannins used to produce the studied decorative vegetable tanned leathers and, at the same time, the detection of chemical deterioration of leathers, particularly collagen hydrolysis, which was not visually perceived.

Urban and spatial planning in Japan

Directory of Open Access Journals (Sweden)

Marin Tominaga

2011-12-01

Full Text Available This paper aims to introduce the urban and spatial planning inJapan. According to the national planning system of Japan, chapter 2, the planning system has 3 administrative levels and each territorial region has its own regulation. This paper introduces especially about planning and regulation system in city region in Japan.

On recycling of nuclear fuel in Japan

International Nuclear Information System (INIS)

1992-01-01

In Japan, atomic energy has become to accomplish the important role in energy supply. Recently the interest in the protection of global environment heightened, and the anxiety on oil supply has been felt due to the circumstances in Mideast. Therefore, the importance of atomic energy as an energy source for hereafter increased, and the future plan of nuclear fuel recycling in Japan must be promoted on such viewpoint. At present in Japan, the construction of nuclear fuel cycle facilities is in progress in Rokkasho, Aomori Prefecture. The prototype FBR 'Monju' started the general functional test in May, this year. The transport of the plutonium reprocessed in U.K. and France to Japan will be carried out in near future. This report presents the concrete measures of nuclear fuel recycling in Japan from the long term viewpoint up to 2010. The necessity and meaning of nuclear fuel recycling in Japan, the effort related to nuclear nonproliferation, the plan of nuclear fuel recycling for hereafter in Japan, the organization of MOX fuel fabrication in Japan and abroad, the method of utilizing recovered uranium and the reprocessing of spent MOX fuel are described. (K.I.)

Liquid hydrogen in Japan

Energy Technology Data Exchange (ETDEWEB)

Yasumi, S. [Iwatani Corp., Osaka (Japan). Dept. of Overseas Business Development

2009-07-01

Japan's Iwatani Corporation has focused its attention on hydrogen as the ultimate energy source in future. Unlike the United States, hydrogen use and delivery in liquid form is extremely limited in the European Union and in Japan. Iwatani Corporation broke through industry stereotypes by creating and building Hydro Edge Co. Ltd., Japan's largest liquid hydrogen plant. It was established in 2006 as a joint venture between Iwatani and Kansai Electric Power Group in Osaka. Hydro Edge is Japan's first combined liquid hydrogen and ASU plant, and is fully operational. Liquid oxygen, liquid nitrogen and liquid argon are separated from air using the cryogenic energy of liquefied natural gas fuel that is used for power generation. Liquid hydrogen is produced efficiently and simultaneously using liquid nitrogen. Approximately 12 times as much hydrogen in liquid form can be transported and supplied as pressurized hydrogen gas. This technology is a significant step forward in the dissemination and expansion of hydrogen in a hydrogen-based economy.

Implementation of the Additional Protocol in Japan

International Nuclear Information System (INIS)

Ogawa, T.

2001-01-01

The Additional Protocol between Japan and the IAEA entered into force in December 1999. To come into force a series of implementation trials of Additional Protocol was carried out at two Japanese representative nuclear research centers, i.e. Tokai Research Establishment of Japan Atomic Energy Research Institute (JAERI) and Oarai Engineering Center of Japan Nuclear Fuel Cycle Development Institute (JNC). These trials were proposed by Japan and were conducted in cooperation with the IAEA Secretariat. In addition, Japan amended 'the Law for the Regulation of Reactors etc.', to collect adequate information to submit to the IAEA, to arrange the surrounding for the complementary access, etc. In addition, Japan Submitted the Initial Declaration of the Additional Protocol within 180 days of the entry into force of the Protocol, in the middle of June, 2000

Proceedings of the Japan-USA Collaborative Workshops on the History of Particle Theory in Japan, 1935-1960

CERN Document Server

Kawabe, Rokuo; Konuma, Michiji; Elementary particle theory in Japan, 1935-1960 : Japan-USA collaboration, second phase

1988-01-01

This volume consists mainly of papers presented at five "workshop", each of two or three days duration, held at various locations in Japan and the United States during 1984-5 by a USA-Japan collaboration for the study of the history of particle physics in Japan, together with some of the discussions and additional interviews.

Nucleofection: A New Method for Cutaneous Gene Transfer?

Directory of Open Access Journals (Sweden)

Frank Jacobsen

2006-01-01

Full Text Available Background. Transfection efficacy after nonviral gene transfer in primary epithelial cells is limited. The aim of this study was to compare transfection efficacy of the recently available method of nucleofection with the established transfection reagent FuGENE6. Methods. Primary human keratinocytes (HKC, primary human fibroblasts (HFB, and a human keratinocyte cell line (HaCaT were transfected with reporter gene construct by FuGENE6 or Amaxa Nucleofector device. At corresponding time points, β-galactosidase expression, cell proliferation (MTT-Test, transduction efficiency (X-gal staining, cell morphology, and cytotoxicity (CASY were determined. Results. Transgene expression after nucleofection was significantly higher in HKC and HFB and detected earlier (3 h vs. 24 h than in FuGENE6. After lipofection 80%–90% of the cells remained proliferative without any influence on cell morphology. In contrast, nucleofection led to a decrease in keratinocyte cell size, with only 20%–42% proliferative cells. Conclusion. Related to the method-dependent increase of cytotoxicity, transgene expression after nucleofection was earlier and higher than after lipofection.

Nucleofection: A New Method for Cutaneous Gene Transfer?

Science.gov (United States)

Jacobsen, Frank; Mertens-Rill, Janine; Beller, Juergen; Hirsch, Tobias; Daigeler, Adrien; Langer, Stefan; Lehnhardt, Marcus; Steinau, Hans-Ulrich; Steinstraesser, Lars

2006-01-01

Background. Transfection efficacy after nonviral gene transfer in primary epithelial cells is limited. The aim of this study was to compare transfection efficacy of the recently available method of nucleofection with the established transfection reagent FuGENE6. Methods. Primary human keratinocytes (HKC), primary human fibroblasts (HFB), and a human keratinocyte cell line (HaCaT) were transfected with reporter gene construct by FuGENE6 or Amaxa Nucleofector device. At corresponding time points, β-galactosidase expression, cell proliferation (MTT-Test), transduction efficiency (X-gal staining), cell morphology, and cytotoxicity (CASY) were determined. Results. Transgene expression after nucleofection was significantly higher in HKC and HFB and detected earlier (3 h vs. 24 h) than in FuGENE6. After lipofection 80%–90% of the cells remained proliferative without any influence on cell morphology. In contrast, nucleofection led to a decrease in keratinocyte cell size, with only 20%–42% proliferative cells. Conclusion. Related to the method-dependent increase of cytotoxicity, transgene expression after nucleofection was earlier and higher than after lipofection. PMID:17489014

Japan's new energy policy

International Nuclear Information System (INIS)

2014-11-01

Japan's energy policy is undergoing fundamental changes. The accident at TEPCO's Fukushima Daiichi nuclear power plant questions the future contribution of nuclear power in the national energy mix. Growing imports of fossil fuels to replace the lost nuclear capacity inflated energy prices and raise economic and energy security challenges. At the same time, the US shale gas and oil revolution is reshaping the global energy scene. Japan expects to take advantage of the trend to eliminate the 'Asian premium' on natural gas prices and expand cheaper natural gas consumption. These developments have driven the Government of Japan to review its energy policy from scratch and adopt a new Strategic Energy Plan. This new policy has far reaching implications for gas and coal development in Japan but also for the international markets as Japan is the world's largest LNG importer and the second largest coal importer. This document summarizes the key findings of a new report by CEDIGAZ 'Japan's new energy policy: In search for stable and competitive energy supply'. The report analyzes the current changes taking place on the gas and coal markets in Japan, in light of the new energy policy adopted in April 2014, and in particular the decision to restart safe nuclear power plants and push forward electricity market reforms

Psychology in Japan.

Science.gov (United States)

Imada, Hiroshi; Tanaka-Matsumi, Junko

2016-06-01

The purpose of this article is to provide information about Japan and its psychology in advance of the 31st International Congress of Psychology (ICP), to be held in Yokohama, Japan, in 2016. The article begins with the introduction of the Japanese Psychological Association (JPA), the hosting organization of the ICP 2016, and the Japanese Union of Psychological Associations consisting of 51 associations/societies, of which the JPA is a member. This is followed by a brief description of a history of psychology of Japan, with emphasis on the variation in our approach to psychology in three different periods, that is, the pre- and post-Pacific War periods, and the post-1960 period. Next, the international contributions of Japanese psychology/psychologists are discussed from the point of view of their visibility. Education and training in psychology in Japanese universities is discussed with a final positive remark about the long-awaited enactment of the Accredited Psychologist Law in September, 2015. © 2016 International Union of Psychological Science.

Chemical transitions of Areca semen during the thermal processing revealed by temperature-resolved ATR-FTIR spectroscopy and two-dimensional correlation analysis

Science.gov (United States)

Wang, Zhibiao; Wang, Xu; Pei, Wenxuan; Li, Sen; Sun, Suqin; Zhou, Qun; Chen, Jianbo

2018-03-01

Areca semen is a common herb used in traditional Chinese medicine, but alkaloids in this herb are categorized as Group I carcinogens by IARC. It has been proven that the stir-baking process can reduce alkaloids in Areca semen while keep the activity for promoting digestion. However, the changes of compositions other than alkaloids during the thermal processing are unclear. Understanding the thermal chemical transitions of Areca semen is necessary to explore the processing mechanisms and optimize the procedures. In this research, FTIR spectroscopy with a temperature-controlled ATR accessory is employed to study the heating process of Areca semen. Principal component analysis and two-dimensional correlation spectroscopy are used to interpret the spectra to reveal the chemical transitions of Areca semen in different temperature ranges. The loss of a few volatile compounds in the testa and sperm happens below 105 °C, while some esters in the sperm decreases above 105 °C. As the heating temperature is close to 210 °C, Areca semen begins to be scorched and the decomposition of many compounds can be observed. This research shows the potential of the temperature-resolved ATR-FTIR spectroscopy in exploring the chemical transitions of the thermal processing of herbal materials.

The Neurospora crassa UVS-3 epistasis group encodes homologues of the ATR/ATRIP checkpoint control system.

Science.gov (United States)

Kazama, Yusuke; Ishii, Chizu; Schroeder, Alice L; Shimada, Hisao; Wakabayashi, Michiyoshi; Inoue, Hirokazu

2008-02-01

The mutagen sensitive uvs-3 and mus-9 mutants of Neurospora show mutagen and hydroxyurea sensitivity, mutator effects and duplication instability typical of recombination repair and DNA damage checkpoint defective mutants. To determine the nature of these genes we used cosmids from a genomic library to clone the uvs-3 gene by complementation for MMS sensitivity. Mutation induction by transposon insertion and RIP defined the coding sequence. RFLP analysis confirmed that this sequence maps in the area of uvs-3 at the left telomere of LG IV. Analysis of the cDNA showed that the UVS-3 protein contains an ORF of 969 amino acids with one intron. It is homologous to UvsD of Aspergillus nidulans, a member of the ATRIP family of checkpoint proteins. It retains the N' terminal coiled-coil motif followed by four basic amino acids typical of these proteins and shows the highest homology in this region. The uvsD cDNA partially complements the defects of the uvs-3 mutation. The uvs-3 mutant shows a higher level of micronuclei in conidia and failure to halt germination and nuclear division in the presence of hydroxyurea than wild type, suggesting checkpoint defects. ATRIP proteins bind tightly to ATR PI-3 kinase (phosphatidylinositol 3-kinase) proteins. Therefore, we searched the Neurospora genome sequence for homologues of the Aspergillus nidulans ATR, UvsB. A uvsB homologous sequence was present in the right arm of chromosome I where the mus-9 gene maps. A cosmid containing this genomic DNA complemented the mus-9 mutation. The putative MUS-9 protein is 2484 amino acids long with eight introns. Homology is especially high in the C-terminal 350 amino acids that correspond to the PI-3 kinase domain. In wild type a low level of constitutive mRNA is present for both genes. It is transiently induced upon UV exposure.

[History of pandemic influenza in Japan].

Science.gov (United States)

Matsumoto, Keizo

2010-09-01

In Japan, influenza like epidemics were described many times since Heian era. However, Spanish flu as the modern medicine invaded Japan in 1918, thus almost infected 390,000 patients died with associated pneumonia. After the discovery of influenza virus in 1933, Japan experienced pandemic influenza--Asian flu(H2N2) in 1957. After about 10 years, Hong Kong flu (H3N2) came to Japan at 1968. However, we had many reliable antibiotics but had not any antiviral drug at the early time. After year 2000, we fortunately obtained reliable three antiviral drugs such as amantadine, oseltamivir and zanamivir. Moreover, very useful rapid test kits for influenza A and B viruses were developed and used in Japan. 2009 H1N1 influenza epidemic occured in Japan after the great epidemic in Mexico and North America but elderly patient was few. With together, host conditions regarding with high risk are changing. Lessons from past several pandemic influenza are those that many issues for changing high risk conditions, viral genetic changes, developing antiviral agents, developing new useful vaccins and determinating bacterial secondary pathogens are important.

IAEA Remediation Mission to Japan Concludes

International Nuclear Information System (INIS)

2011-01-01

Full text: A team of international experts today completed a preliminary assessment of the strategy and plans being considered by the Japanese authorities to remediate the areas off-site the Fukushima Dai-ichi Nuclear Power Plant reported to have elevated levels of radiation. The IAEA dispatched the mission to Japan on 7 October following a request from the country's Government. The mission, comprising 12 international and IAEA experts from several countries, visited numerous locations in the Fukushima Prefecture and conducted meetings in Tokyo and Fukushima with Japanese officials from several Ministries and institutions. ''The meetings held and visits made by the team over the last eight days gave us a first-hand appreciation of the extraordinary efforts and dedication on the part of Japanese people in their effort to remediate the areas affected by elevated levels of radiation in the Fukushima Prefecture,'' says Mr. Juan Carlos Lentijo, Team Leader and General Director for Radiation Protection at Spain's nuclear regulatory authority. ''As Japan continues its current remediation efforts, it is our belief that this work will bring relief to the populations who are affected by the consequences of the nuclear accident at the Fukushima Dai-ichi nuclear power plant.'' In a Preliminary Summary Report delivered to Japanese authorities today, the team prepared a set of conclusions including, though not limited to, the following: - Japan developed an efficient program for remediation - allocating the necessary legal, financial and technological resources to bring relief to the people affected by the accident, with priority being given to children. The Team was impressed with the strong commitment to the remediation effort from all institutions and parties involved, including the public; - Japan has also taken practical measures to inform the public and involve residents and local institutions in the process of defining its remediation strategy; - Japan is advised to avoid

A new integrated TLC/MU-ATR/SERS advanced approach for the identification of trace amounts of dyes in mixtures.

Science.gov (United States)

Sciutto, Giorgia; Prati, Silvia; Bonacini, Irene; Litti, Lucio; Meneghetti, Moreno; Mazzeo, Rocco

2017-10-23

The present research is focused on the setting up of an advanced analytical system for the detection of synthetic dyes. The system is based on the combination of an innovative thin layer chromatography (TLC) plate coupled with enhanced infrared (MU-ATR, metal underlayer attenuated total reflection) and Surface Enhanced Raman (SERS) spectroscopy. In particular, a TLC plate made of silver iodide (AgI) applied onto a gold coated glass slide (AgI@Au) is proposed as an efficient stationary phase for the separation of dyes mixtures. The separated dyes are then identified by means of both enhanced FTIR and SERS, performed directly on the same eluted spots. The use of a mid-IR transparent inorganic salt as stationary phase coupled with the underneath gold layer avoids spectral interferences, enhancing the signal obtained from ATR analyses. At the same time, SERS spectra can be recorded as the TLC plate may act as a SERS active substrate due to the photoreduction of AgI to metallic Ag caused by the exposure to the laser during the Raman analysis. Different mixtures of synthetic dyes of known composition, widely used in dyeing processes, have been tested and the method resulted to be effective in identifying trace amounts in the order of tens nanograms. Moreover, the method has been further evaluated on a real case study represented by dyes extracted from dyed wool. Copyright © 2017 Elsevier B.V. All rights reserved.

75 FR 38119 - Polychloroprene Rubber From Japan

Science.gov (United States)

2010-07-01

... Rubber From Japan AGENCY: United States International Trade Commission. ACTION: Institution of a five-year review concerning the antidumping duty finding on polychloroprene rubber from Japan. SUMMARY: The... on polychloroprene rubber from Japan would be likely to lead to continuation or recurrence of...

Electronic manufacturing and packaging in Japan

Science.gov (United States)

Kelly, Michael J.; Boulton, William R. (Editor); Kukowski, John A.; Meieran, Eugene S.; Pecht, Michael; Peeples, John W.; Tummala, Rao R.

1995-01-01

This report summarizes the status of electronic manufacturing and packaging technology in Japan in comparison to that in the United States, and its impact on competition in electronic manufacturing in general. In addition to electronic manufacturing technologies, the report covers technology and manufacturing infrastructure, electronics manufacturing and assembly, quality assurance and reliability in the Japanese electronics industry, and successful product realization strategies. The panel found that Japan leads the United States in almost every electronics packaging technology. Japan clearly has achieved a strategic advantage in electronics production and process technologies. Panel members believe that Japanese competitors could be leading U.S. firms by as much as a decade in some electronics process technologies. Japan has established this marked competitive advantage in electronics as a consequence of developing low-cost, high-volume consumer products. Japan's infrastructure, and the remarkable cohesiveness of vision and purpose in government and industry, are key factors in the success of Japan's electronics industry. Although Japan will continue to dominate consumer electronics in the foreseeable future, opportunities exist for the United States and other industrial countries to capture an increasingly large part of the market. The JTEC panel has identified no insurmountable barriers that would prevent the United States from regaining a significant share of the consumer electronics market; in fact, there is ample evidence that the United States needs to aggressively pursue high-volume, low-cost electronic assembly, because it is a critical path leading to high-performance electronic systems.

Information on research in progress in Japan publication of 'Nuclear Science Information of Japan-Oral Presentation'

International Nuclear Information System (INIS)

Itabashi, Keizo; Nakajima, Hidemitsu; Yokoo, Hiroshi

1988-01-01

The new journal, 'Nuclear Science Information of Japan-Oral Presentation', which was entirely revised from the previous abstract journal called 'Nuclear Science Information of Japan' was introduced. This is to be published quaterly in principle and compiled information by oral presentation presented at main conferences, symposia and other kind of formal meetings in the field of nuclear science and technology in Japan. Not all of the oral presentation is always contributed later to a proceeding or a journal as a full paper in Japan. In some cases, the pre-conference paper might be a only publication of the oral presentation. In this meaning, this journal could be used as a search tool for the subjects and the projects of nuclear research and development in progress. (author)

Electronics manufacturing and assembly in Japan

Science.gov (United States)

Kukowski, John A.; Boulton, William R.

1995-02-01

In the consumer electronics industry, precision processing technology is the basis for enhancing product functions and for minimizing components and end products. Throughout Japan, manufacturing technology is seen as critical to the production and assembly of advanced products. While its population has increased less than 30 percent over twenty-five years, Japan's gross national product has increase thirtyfold; this growth has resulted in large part from rapid replacement of manual operations with innovative, high-speed, large-scale, continuously running, complex machines that process a growing number of miniaturized components. The JTEC panel found that introduction of next-generation electronics products in Japan goes hand-in-hand with introduction of new and improved production equipment. In the panel's judgment, Japan's advanced process technologies and equipment development and its highly automated factories are crucial elements of its domination of the consumer electronics marketplace - and Japan's expertise in manufacturing consumer electronics products gives it potentially unapproachable process expertise in all electronics markets.

Transport of MOX fuel from Europe to Japan

International Nuclear Information System (INIS)

2002-01-01

The MOX fuel transports from Europe to Japan represent a main part in the implementing of the Japan nuclear program. They complement the 160 transports of spent fuels realized from Japan to Europe and the vitrified residues return from France to Japan. In this framework the document presents the MOX fuel, the use of the MOX fuel in reactor, the proliferation risks, the MOX fuel transport to Japan, the public health, the transport regulations, the safety and the civil liability. (A.L.B.)

The Early Overseas Activities of IBM in Japan

OpenAIRE

吉沢, 正広; 田中, 雅章; Masahiro, YOSHIZAWA; Masaaki, TANAKA; 愛知学院大学; 鈴鹿短期大学; / SUZUKA JUNIOR COLLEGE

1998-01-01

The purpose of this paper is to examine how IBM entered into prewar Japan and ran its wholly-owned subsidiary thereafter. Before examining something noted above, this paper surveys the process of formation of IBM and then traces its early overseas activities. Turning to the initial contacts between Japan and IBM, at first IBM carried out its mere export trade to Japan through Morimura and then Kurosawa. IBM then established its wholly-owned subsidiary, Japan Watson,in Japan to expand the busi...

Non-apoptotic programmed cell death with paraptotic-like features in bleomycin-treated plant cells is suppressed by inhibition of ATM/ATR pathways or NtE2F overexpression

Czech Academy of Sciences Publication Activity Database

Smetana, O.; Široký, Jiří; Houlné, G.; Opatrný, Z.; Chabouté, M.-E.

2012-01-01

Roč. 63, č. 7 (2012), s. 2631-2644 ISSN 0022-0957 Institutional research plan: CEZ:AV0Z50040702 Keywords : ATM /ATR pathways * cell cycle * double-strand break response Subject RIV: BO - Biophysics Impact factor: 5.242, year: 2012

Epidemiology of diabetes mellitus in Japan

Energy Technology Data Exchange (ETDEWEB)

Blackard, W G; Omori, Yoshiaki; Freedman, L R

1964-03-12

The clinical and epidemiological features of diabetes mellitus in Japan have been compiled and compared with data from other countries. Diabetes is basically the same in Japan as elsewhere: however, consideration of important differences has led to the following conclusions: The rarity of ketoacidosis in Japan is due to the mild carbohydrate defect present in most diabetics. The mild carbohydrate intolerance in diabetics is probably in part due to a high carbohydrate intake. Diabetic retinopathy is more common in women than in men in Japan; there are limited and conflicting data from the West on this point, but retinopathy, nephropathy and neuropathy occur about as frequently in Japan as in the West. Because of marked dietary differences between Japan and Western countries, these findings suggest that dietary fat has no significance in the pathogenesis of these lesions. Peripheral gangrene is distinctly unusual in Japanese diabetics. This suggests that either: the responsible vascular lesions are different from those responsible for nephropathy and retinopathy; or that small vessel lesions are the same but the lack of large vessel atherosclerosis in the population accounts for the decreased incidence of gangrene. Men have diabetes 2 or 3 times as commonly as women in Japan. If sex-limited inheritance is discarded as a possible reason, it is likely that adult-onset diabetes is more common in men than women except in those countries (the West) where women gain relatively large amounts of weight. The rarity of juvenile diabetes in Japan is best explained by the infrequency of responsible genetic factors. As a consequence, it is likely that juvenile diabetes is caused by different or additional genetic factors which are not significant in adult-onset diabetes. Diabetes prevalance varies sufficiently between different localities in the same country to render the concept of national prevalance of doubtful usefulness. 55 references, 3 figures, 3 tables.

Influence of alkali metal cations/type of activator on the structure of alkali-activated fly ash - ATR-FTIR studies

Science.gov (United States)

Król, M.; Rożek, P.; Chlebda, D.; Mozgawa, W.

2018-06-01

Coal fly ash as a secondary aluminosiliceous raw material that is commonly used in the so-called geopolymerization process has been activated with different alkali hydroxides solutions: LiOH, NaOH and KOH. Changes in the aluminosilicate structure of the material during alkali-activation have been analyzed in detail on the basis of ATR/FT-IR spectra. These changes mainly affect both the integral intensity and FWHM of bands in the range of 1200-950 cm-1, however dehydration and carbonation process can be also analyzed based on obtaining results.

Recent developments: Japan and Australia

International Nuclear Information System (INIS)

Anon.

1990-01-01

Recent developments in the nuclear industry in Japan and Australia are briefly reviewed. Topics discussed include: the world energy situation; and nuclear power generation trends and completion the nuclear fuel cycle in Japan. Recent events that suggest possible policy changes in Australia are briefly discussed

The Cogema group in Japan

International Nuclear Information System (INIS)

1998-12-01

The partnership between the Cogema group and Japan in the domain of fuel cycle started about 20 years ago and the 10 Japanese nuclear operators are all clients of the Cogema group. The 1997 turnover realized with Japan reached 3.6 billions of francs (11% of the total turnover of the group). This short paper presents briefly the nuclear program of Japan (nuclear park, spent fuels reprocessing-recycling strategy) and the contracts between Cogema and the Japanese nuclear operators (natural uranium, uranium conversion and enrichment, spent fuel reprocessing, plutonium recycle and MOX fuel production markets). (J.S.)

Study of the interface solid/solutions containing PEO-PPO block copolymers and asphaltenes by FTIR/ATR; Estudo de solucoes de copolimeros em bloco de PEO-PPO contendo asfaltenos por FTIR/DTA

Energy Technology Data Exchange (ETDEWEB)

Aguiar, Janaina I.S.; Neto, Jessica S.G.; Mansur, Claudia R.E. [Universidade Federal do Rio de Janeiro, Instituto de Macromoleculas, Laboratorio de Macromoleculas e Coloides na Industria de Petroleo, Rio de Janeiro, RJ (Brazil)], E-mails: janaina_333@hotmail.com, kinha_dac_dm@hotmail.com; celias@ima.ufrj.br

2011-07-01

The formation of water/oil emulsions can cause problems in various stages of production, processing and refining of petroleum. In this study, the technique of Fourier transform infrared spectroscopy (FTIR) using the method of attenuated total reflectance (ATR) was applied to study the solid-solutions of block copolymers based on poly(ethylene oxide)-poly(propylene oxide) (PEO-PPO) interface and its interaction in this interface with asphaltenic fractions of petroleum. The solid is the crystal of the ATR. Initially, we determined the critical micelle concentration values of the copolymers, which were consistent those obtained by a tensiometer. Bottle Test was also performed to correlate the efficiency of PEO-PPO copolymers in the breaking of water/oil emulsions with its adsorption at the interfaces solutions. (author)

Tokai works semi-annual progress report, July--December 1975

International Nuclear Information System (INIS)

1976-09-01

The Power Reactor and Nuclear Fuel Development Corporation (PNC) is a semi-governmental organization responsible for the development of advanced power reactors and nuclear fuels in Japan. The Tokai Works is the PNC center for research and development of nuclear fuels concerned with plutonium fuels fabrication, fuel reprocessing, and centrifugal uranium enrichment. Accomplishments in the activities of Tokai Works during the latter half of 1975 are summarized as follows: (1) Plutonium fuels development--Fabrication of core fuel assemblies is being continued for initial loading of the Experimental Fast Breeder Reactor JOYO and remodeling is being carried out on the facility for fabrication of plutonium fuels for the Prototype Heavy Water Moderated and Boiling Light Water Cooled Reactor FUGEN; (2) Fuel reprocessing--Construction of the Tokai Reprocessing Plant is nearly completed and preparation for its commissioning is being made; (3) Development of centrifugal uranium enrichment is being performed successfully

Nuclear reactor control with fuzzy logic approaches - strengths, weakness, opportunities, and threats

International Nuclear Information System (INIS)

Ruan, Da

2004-01-01

As part of the special track on 'Lessons learned from computational intelligence in nuclear applications' at the forthcoming FLINS 2004 conference on Applied Computational Intelligence (Blankenberge, Belgium, September 1-3, 2004), research experiences on fuzzy logic techniques in applications of nuclear reactor control operation are critically reviewed in this presentation. Assessment of four real fuzzy control applications at the MIT research reactor in the US, the FUGEN heavy water reactor in Japan, the BR1 research reactor in Belgium, and a TRIGA Mark III reactor in Mexico will be examined thought a SWOT analysis (strengths, weakness, opportunities, and threats). Special attention will be paid to the current cooperation between the Belgian Nuclear Research Centre (SCK-CEN) and the Mexican Nuclear Centre (ININ) on the fuzzy logic control for nuclear reactor control project under the partial support of the National Council for Science and Technology of Mexico (CONACYT). (Author)

Quantitative determination of polyphosphate in sediments using Attenuated Total Reflectance-Fourier Transform Infrared (ATR-FTIR) spectroscopy and partial least squares regression.

Science.gov (United States)

Khoshmanesh, Aazam; Cook, Perran L M; Wood, Bayden R

2012-08-21

Phosphorus (P) is a major cause of eutrophication and subsequent loss of water quality in freshwater ecosystems. A major part of the flux of P to eutrophic lake sediments is organically bound or of biogenic origin. Despite the broad relevance of polyphosphate (Poly-P) in bioremediation and P release processes in the environment, its quantification is not yet well developed for sediment samples. Current methods possess significant disadvantages because of the difficulties associated with using a single extractant to extract a specific P compound without altering others. A fast and reliable method to estimate the quantitative contribution of microorganisms to sediment P release processes is needed, especially when an excessive P accumulation in the form of polyphosphate (Poly-P) occurs. Development of novel approaches for application of emerging spectroscopic techniques to complex environmental matrices such as sediments significantly contributes to the speciation models of P mobilization, biogeochemical nutrient cycling and development of nutrient models. In this study, for the first time Attenuated Total Reflectance-Fourier Transform Infrared (ATR-FTIR) spectroscopy in combination with partial least squares (PLS) was used to quantify Poly-P in sediments. To reduce the high absorption matrix components in sediments such as silica, a physical extraction method was developed to separate sediment biological materials from abiotic particles. The aim was to achieve optimal separation of the biological materials from sediment abiotic particles with minimum chemical change in the sample matrix prior to ATR-FTIR analysis. Using a calibration set of 60 samples for the PLS prediction models in the Poly-P concentration range of 0-1 mg g(-1) d.w. (dry weight of sediment) (R(2) = 0.984 and root mean square error of prediction RMSEP = 0.041 at Factor-1) Poly-P could be detected at less than 50 μg g(-l) d.w. Using this technique, there is no solvent extraction or chemical

Research of fault activity in Japan

International Nuclear Information System (INIS)

Nohara, T.; Nakatsuka, N.; Takeda, S.

2004-01-01

Six hundreds and eighty earthquakes causing significant damage have been recorded since the 7. century in Japan. It is important to recognize faults that will or are expected to be active in future in order to help reduce earthquake damage, estimate earthquake damage insurance and siting of nuclear facilities. Such faults are called 'active faults' in Japan, the definition of which is a fault that has moved intermittently for at least several hundred thousand years and is expected to continue to do so in future. Scientific research of active faults has been ongoing since the 1930's. Many results indicated that major earthquakes and fault movements in shallow crustal regions in Japan occurred repeatedly at existing active fault zones during the past. After the 1995 Southern Hyogo Prefecture Earthquake, 98 active fault zones were selected for fundamental survey, with the purpose of efficiently conducting an active fault survey in 'Plans for Fundamental Seismic Survey and Observation' by the headquarters for earthquake research promotion, which was attached to the Prime Minister's office of Japan. Forty two administrative divisions for earthquake disaster prevention have investigated the distribution and history of fault activity of 80 active fault zones. Although earthquake prediction is difficult, the behaviour of major active faults in Japan is being recognised. Japan Nuclear Cycle Development Institute (JNC) submitted a report titled 'H12: Project to Establish the. Scientific and Technical Basis for HLW Disposal in Japan' to the Atomic Energy Commission (AEC) of Japan for official review W. The Guidelines, which were defined by AEC, require the H12 Project to confirm the basic technical feasibility of safe HLW disposal in Japan. In this report the important issues relating to fault activity were described that are to understand the characteristics of current fault movements and the spatial extent and magnitude of the effects caused by these movements, and to

Evaluation of Corrosion of the Dummy ''EE'' Plate 19 in YA Type ATR Fuel Element During Reactor PALM Cycles

International Nuclear Information System (INIS)

Brower, Jeffrey Owen; Glazoff, Michael Vasily; Eiden, Thomas John; Rezvoi, Aleksey Victor

2016-01-01

Advanced Test Reactor (ATR) Cycle 153B-1 was a 14-day, high-power, powered axial locator mechanism (PALM) operating cycle that completed on April 12, 2013. Cycle 153B-1 was a typical operating cycle for the ATR and did not result in any unusual plant transients. ATR was started up and shut down as scheduled. The PALM drive physically moves the selected experiments into and out of the core to simulate reactor startup and heat up, and shutdown and cooldown transients, while the reactor remains in steady state conditions. However, after the cycle was over, several thousand of the flow-assisted corrosion pits and ''horseshoeing'' defects were readily observable on the surface of the several YA-type fuel elements (these are ''dummy'' plates that contain no fuel). In order understand these corrosion phenomena a thermal-hydraulic model of coolant channel 20 on a YA-M fuel element was generated. The boundaries of the model were the aluminum EE plate of a YA-M fuel element and a beryllium reflector block with 13 horizontal saw cuts which represented regions of zero flow. The heat generated in fuel plates 1 through 18 was modeled to be passing through the aluminum EE plate. The coolant channel 20 width was set at 0.058 in. (58 mils). It was established that the horizontal saw cuts had a significant effect on the temperature of the coolant. The flow, which was expected to vary linearly with gradual heating of the coolant as it passed through the channel, was extremely turbulent. The temperature rise, which was expected to be a smooth ''S'' curve, was represented by a series temperature rise ''humps,'' which occurred at each horizontal saw cut in the beryllium reflector block. Each of the 13 saw cuts had a chamfered edge which resulted in the coolant flow being re-directed as a jet across the coolant channel into the surface of the EE plate, which explained the temperature rise and the observed sscalloping and possibly pitting degradation on the YA-M fuel elements. In the case

Analysis of energy intensity in Japan

International Nuclear Information System (INIS)

Okajima, Shigeharu; Okajima, Hiroko

2013-01-01

This study discusses the causes of the increase in Japan's energy intensity, defined as energy consumption divided by GDP, since the early 1990s. The significant reduction in Japan's energy intensity ceased in the early 1980s and has even slightly increased since the early 1990s, indicating that Japan seemingly stopped taking aggressive action to improve energy use. However, further analysis at prefecture level and sector level provides additional insight on energy intensity trends. To analyze the causes of the increase in Japan's energy intensity, energy intensity is decomposed into energy efficiency (improvements in energy efficiency) and energy activity (structural changes from the secondary sector to the tertiary sector of the economy). Our result indicates that the non-uniform energy intensity trends between prefectures are attributed to a high variability in energy efficiency. At sector level, we estimate the income elasticity of energy consumption in each sector and find that a structural change in energy consumption behaviors occurred in all sectors at different time points. The industrial sector and commercial sector became less energy efficient after 1981 and 1988, respectively, which is presumably responsible for the deterioration of Japan's energy intensity since the early 1990s. - Highlights: • We examine why the reduction in Japan's energy intensity increased in the early 1990s. • There is a high variability in energy intensity trends between regions. • The structural changes in energy consumption behaviors occurred in sector level. • These changes may be responsible for the deterioration of Japan's energy intensity

Urgent Safety Measures in Japan after Great East Japan Earthquake

International Nuclear Information System (INIS)

Taniura, W.; Otani, H.

2012-01-01

Due to tsunami triggered by the Great East Japan Earthquake on March 11, 2011, the operating and refueling reactor facilities at Fukushima Dai-ichi and Dai-ni Nuclear Power Plants of Tokyo Electric Power Co. caused a nuclear hazard. Japanese electric power companies voluntarily began to compile various urgent measures against tsunami within the week the hazard was caused. As for the urgent safety measures of each licensee, it is clarified that effective measures have been appropriately implemented as a result of the inspection of the national government, the verification based on the guideline of the Japan Society of Maintenology and the stress test. (author)

Recent trends for drug lag in clinical development of oncology drugs in Japan: does the oncology drug lag still exist in Japan?

Science.gov (United States)

Maeda, Hideki; Kurokawa, Tatsuo

2015-12-01

This study exhaustively and historically investigated the status of drug lag for oncology drugs approved in Japan. We comprehensively investigated oncology drugs approved in Japan between April 2001 and July 2014, using publicly available information. We also examined changes in the status of drug lag between Japan and the United States, as well as factors influencing drug lag. This study included 120 applications for approval of oncology drugs in Japan. The median difference over a 13-year period in the approval date between the United States and Japan was 875 days (29.2 months). This figure peaked in 2002, and showed a tendency to decline gradually each year thereafter. In 2014, the median approval lag was 281 days (9.4 months). Multiple regression analysis identified the following potential factors that reduce drug lag: "Japan's participation in global clinical trials"; "bridging strategies"; "designation of priority review in Japan"; and "molecularly targeted drugs". From 2001 to 2014, molecularly targeted drugs emerged as the predominant oncology drug, and the method of development has changed from full development in Japan or bridging strategy to global simultaneous development by Japan's taking part in global clinical trials. In line with these changes, the drug lag between the United States and Japan has significantly reduced to less than 1 year.

Food Irradiation In Vietnam And Japan

International Nuclear Information System (INIS)

Tamikazy Kume

2011-01-01

In 2008, Japan Atomic Energy Commission of Cabinet Office performed the study of current status of food irradiation in the world. The results showed that the total quantity of irradiated foods in 2005 was 405,000 tons. Seven main countries for food irradiation were China, USA, Ukraine, Brazil, South Africa, Vietnam and Japan. In Japan, only the potato irradiation for sprout inhibition is continued more than 35 years since 1974 but the quantity is decreasing. On the other hand, the food irradiation of Vietnam has been developed rapidly in a short time to export the frozen seafood and fruit. This paper shows the status of food irradiation in Vietnam and Japan, and the progress in both countries after 2005. (author)

Japan's National Security: Structures, norms, and policies

International Nuclear Information System (INIS)

Katzenstein, P.J.; Okawara, N.

1993-01-01

Japan's national security policy has two distinctive aspects that deserve analysis. First, Japan's definition of national security goes far beyond traditional military notions. National security is viewed in comprehensive terms that also include economic and political dimensions. The second feature of Japan's security policy worth explanation is a distinctive mixture of flexibility and rigidity in the process of policy adaptation to change: flexibility on issues of economic security, rigidity on issues of military security, and flexibility combined with rigidity on issues of political security. With the end of the Cold War and changes in the structure of the international system, it is only natural that we ask whether and how Japan's national security policy will change as well. Optimists insist that the Asian balance of power and the US-Japan relationship will make Japan aspire to be a competitive, noninterventionist trading state that heeds the universal interest of peace and profit rather than narrow aspirations for national power. Pessimists warn us instead that the new international system will finally confirm Herman Kahn's prediction of 1970: Japan will quickly change to the status of a nuclear superpower, spurred perhaps by what some see as a dangerous rise of Japanese militarism in the 1970s and 1980s

Source Determination of Red Gel Pen Inks using Raman Spectroscopy and Attenuated Total Reflectance Fourier Transform Infrared Spectroscopy combined with Pearson's Product Moment Correlation Coefficients and Principal Component Analysis.

Science.gov (United States)

Mohamad Asri, Muhammad Naeim; Mat Desa, Wan Nur Syuhaila; Ismail, Dzulkiflee

2018-01-01

The potential combination of two nondestructive techniques, that is, Raman spectroscopy (RS) and attenuated total reflectance-fourier transform infrared (ATR-FTIR) spectroscopy with Pearson's product moment correlation (PPMC) coefficient (r) and principal component analysis (PCA) to determine the actual source of red gel pen ink used to write a simulated threatening note, was examined. Eighteen (18) red gel pens purchased from Japan and Malaysia from November to December 2014 where one of the pens was used to write a simulated threatening note were analyzed using RS and ATR-FTIR spectroscopy, respectively. The spectra of all the red gel pen inks including the ink deposited on the simulated threatening note gathered from the RS and ATR-FTIR analyses were subjected to PPMC coefficient (r) calculation and principal component analysis (PCA). The coefficients r = 0.9985 and r = 0.9912 for pairwise combination of RS and ATR-FTIR spectra respectively and similarities in terms of PC1 and PC2 scores of one of the inks to the ink deposited on the simulated threatening note substantiated the feasibility of combining RS and ATR-FTIR spectroscopy with PPMC coefficient (r) and PCA for successful source determination of red gel pen inks. The development of pigment spectral library had allowed the ink deposited on the threatening note to be identified as XSL Poppy Red (CI Pigment Red 112). © 2017 American Academy of Forensic Sciences.

In Defense of Japan

Science.gov (United States)

2018-01-03

potential addition of F-35B STOVL aircraft to their Izumo class helicopter destroyers is a good example. Japan’s conduct during World War II remain locked ...antiwar and antinuclear identity . But after sixty years, Japan’s neighbors still see bayoneted babies. Relations between Japan and its former victims...and the world order Robert Cooper defines Japan as the lone post-modern country surrounded by states firmly locked into an earlier age and that if

Legislative Basis of Pedagogical Education in Japan

Science.gov (United States)

Kuchai, Tetiana

2014-01-01

Legal framework policy of Japan in the field of education has been analyzed. The problem of influence of legislative materials on the development of education in Japan, its legislative support has been considered. It has been defined that directive materials affect the development of education system in Japan. Legislation policy of the country is…

Formulation and drug-content assay of microencapsulated antisense oligonucleotide to NF-κB using ATR-FTIR

International Nuclear Information System (INIS)

Siwale, Rodney; Meadows, Fred; Mody, Vicky V; Shah, Samit

2013-01-01

Antisense oligonucleotide to NF-κB sequence: 5′-GGA AAC ACA TCC TCC ATG-3′, was microencapsulated in an albumin matrix by the method of spray drying TM . Spectral analysis was performed on varying drug loading formulations of both drugs by mid-IR attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR). An out of plane O–H bending vibration at 948 cm −1 , unique to both the native and microencapsulated drugs was identified. The calculated peak areas corresponded to the drug loadings in the microsphere formulations. A standard curve could then be used to determine the drug content of an unknown microsphere formulation. Accuracy and precision were determined to be comparable to other analytical techniques such as HPLC. (paper)

Quantitative analysis of anti-inflammatory drugs using FTIR-ATR spectrometry

Science.gov (United States)

Hassib, Sonia T.; Hassan, Ghaneya S.; El-Zaher, Asmaa A.; Fouad, Marwa A.; Taha, Enas A.

2017-11-01

Four simple, accurate, sensitive and economic Attenuated Total Reflectance-Fourier Transform Infrared Spectroscopic (ATR-FTIR) methods have been developed for the quantitative estimation of some non-steroidal anti-inflammatory drugs. The first method involves the determination of Etodolac by direct measurement of the absorbance at 1716 cm- 1. In the second method, the second derivative of the IR spectra of Tolfenamic acid and its reported degradation product (2-chlorobenzoic acid) was used and the amplitudes were measured at 1084.27 cm- 1 and 1056.02 cm- 1 for Tolfenamic acid and 2-chlorobenzoic acid, respectively. The third method used the first derivative of the IR spectra of Bumadizone and its reported degradation product, N,N-diphenylhydrazine and the amplitudes were measured at 2874.98 cm- 1 and 2160.32 cm- 1 for Bumadizone and N,N-diphenylhydrazine, respectively. The fourth method depends on measuring the amplitude of Diacerein at 1059.18 cm- 1 and of rhein, its reported degradation product, at 1079.32 cm- 1 in their first derivative spectra. The four methods were successfully applied on the pharmaceutical formulations by extracting the active constituent in chloroform and the extract was directly measured in liquid phase mode using a specific cell. Moreover, validation of these methods was carried out following International Conference of Harmonisation (ICH) guidelines.

In situ ATR FTIR studies of SO4 adsorption on goethite in the presence of copper ions.

Science.gov (United States)

Beattie, D A; Chapelet, J K; Gräfe, M; Skinner, W M; Smith, E

2008-12-15

Despite the existence of many single ion sorption studies on iron and aluminum oxides, fewer studies have been reported that describe cosorption reactions. In this work, we present an in situ ATR FTIR study of synergistic adsorption of sulfate (SO4) and copper (Cu) on goethite, which is representative of the minerals and ions present in mine wastes, acid sulfate soils, and other industrial and agricultural settings. Sulfate adsorption was studied as a function of varying pH, and as a function of increasing concentration in the absence and presence of Cu. The presence of Cu ions in solution had a complex effect on the ability of SO4 ions to be retained on the goethite surface with increasing pH, with complete desorption occurring near pH 7 and 9 in the absence and presence of Cu, respectively. In addition, Cu ions altered the balance of inner vs outer sphere adsorbed SO4. The solid phase partitioning of SO4 at pH 3 and pH 5 was elevated by the presence of Cu; in both cases Cu increased the affinity of SO4 for the goethite surface. Complementary ex situ sorption edge studies of Cu on goethite in the absence and presence of SO4 revealed that the Cu adsorption edge shifted to lower pH (6.3 --> 5.6) in the presence of SO4, consistent with a decrease of the electrostatic repulsion between the goethite surface and adsorbing Cu. Based on the ATR FTIR and bulk sorption data we surmise that the cosorption products of SO4 and Cu at the goethite-water interface were not in the nature of ternary complexes under the conditions studied here. This information is critical for the evaluation of the onset of surface precipitates of copper-hydroxy sulfates as a function of pH and solution concentration.

Japan's contribution to nuclear medical research

International Nuclear Information System (INIS)

Rahman, M.; Sakamoto, Junichi; Fukui, Tsuguya

2002-01-01

We investigated the degree of Japan's contribution to the nuclear medical research in the last decade. Articles published in 1991-2000 in highly reputed nuclear medical journals were accessed through the MEDLINE database. The number of articles having affiliation with a Japanese institution was counted along with publication year. In addition, shares of top-ranking countries were determined along with their trends over time. Of the total number of articles (7,788), Japan's share of articles in selected nuclear medical journals was 11.4% (889 articles) and ranked 2nd in the world after the USA (2,645 articles). The recent increase in the share was statistically significant for Japan (p=0.02, test for trend). Japan's share in nuclear medical research output is much higher than that in other biomedical fields. (author)

Kaposi sarcoma herpes virus latency associated nuclear antigen protein release the G2/M cell cycle blocks by modulating ATM/ATR mediated checkpoint pathway.

Directory of Open Access Journals (Sweden)

Amit Kumar

Full Text Available The Kaposi's sarcoma-associated herpesvirus infects the human population and maintains latency stage of viral life cycle in a variety of cell types including cells of epithelial, mesenchymal and endothelial origin. The establishment of latent infection by KSHV requires the expression of an unique repertoire of genes among which latency associated nuclear antigen (LANA plays a critical role in the replication of the viral genome. LANA regulates the transcription of a number of viral and cellular genes essential for the survival of the virus in the host cell. The present study demonstrates the disruption of the host G2/M cell cycle checkpoint regulation as an associated function of LANA. DNA profile of LANA expressing human B-cells demonstrated the ability of this nuclear antigen in relieving the drug (Nocodazole induced G2/M checkpoint arrest. Caffeine suppressed nocodazole induced G2/M arrest indicating involvement of the ATM/ATR. Notably, we have also shown the direct interaction of LANA with Chk2, the ATM/ATR signalling effector and is responsible for the release of the G2/M cell cycle block.

Japan-U.S. cooperation in transport of plutonium

International Nuclear Information System (INIS)

Takeda, Yu

2012-01-01

Japan's effort to utilize plutonium for peaceful purpose since 1950s was caught in a cross fire after the end of the Cold War. Maritime transport of plutonium from France to Japan in 1993, for instance, was criticized by U.S. Congress, nonproliferation specialists and environmental activists. U.S. government, however, compiled with Japan-US Nuclear Cooperation Agreement and cooperated to ship plutonium. This paper focuses on why Washington was supportive for the sealift of plutonium despite of opposition against it. By solving the puzzle, this research will contribute to studies of Japan's plutonium policy and Japan-U.S. nuclear relations. Based on newspaper articles, memoirs and official documents, this paper examines backgrounds and features of controversy surrounding the transport. The analyses show the causes of U.S. cooperation to the transport, such as Japan's efforts toward nuclear nonproliferation. (author)

Attenuated Total Reflection Fourier Transform Infrared (ATR FT-IR) Spectroscopy as a Forensic Method to Determine the Composition of Inks Used to Print the United States One-cent Blue Benjamin Franklin Postage Stamps of the 19th Century.

Science.gov (United States)

Brittain, Harry G

2016-01-01

Through the combined use of infrared (IR) absorption spectroscopy and attenuated total reflectance (ATR) sampling, the composition of inks used to print the many different types of one-cent Benjamin Franklin stamps of the 19th century has been established. This information permits a historical evaluation of the formulations used at various times, and also facilitates the differentiation of the various stamps from each other. In two instances, the ink composition permits the unambiguous identification of stamps whose appearance is identical, and which (until now) have only been differentiated through estimates of the degree of hardness or softness of the stamp paper, or through the presence or absence of a watermark in the paper. In these instances, the use of ATR Fourier transform infrared spectroscopy (FT-IR) spectroscopy effectively renders irrelevant two 100-year-old practices of stamp identification. Furthermore, since the use of ATR sampling makes it possible to obtain the spectrum of a stamp still attached to its cover, it is no longer necessary to identify these blue Franklin stamps using their cancellation dates. © The Author(s) 2015.

Japan and atomic co-operation

International Nuclear Information System (INIS)

1965-01-01

Japan, which is host country for the Ninth Regular Session of the Agency General Conference, has an important programme of nuclear power development to meet future needs. In addition, Japan is active in other applications of atomic energy and is building up a domestic nuclear engineering industry. Japan has profited by the Agency as a channel of international cooperation, and was a party to the first bilateral agreement in which the responsibility for administering safeguards against the diversion of materials to military purposes, was transferred to the Agency. Japan has also lent support to Agency programmes by gifts, training courses, research, and the loan of experts. In 1961, the Japan Atomic Energy Commission (AEC) formulated the 'Long-Range Programme for Development and Utilization of Atomic Energy', on the basis of the economic prospects of nuclear power generation, and the conditions necessary to meet the ever-increasing domestic energy demands. According to this programme, in the light of power reactor development trends overseas, it is expected that nuclear power costs will compete with those of oil burning stations by 1970. On this basis, total nuclear power generating capacity of 1000 MW(e) will be attained by 1970, and 7000 - 9 500M(e) by 1980. As a prelude to the above programme the Japan Atomic Power Company (JAPCO) began construction in 1959 of a graphite-moderated gas-cooled nuclear power station (Improved Calder Hall type) of 165 MW(e) gross capacity. This is now progressing smoothly, and reached criticality in May 1965; it is expected to supply commercial power by the end of this year. The second nuclear power station will be built by the same company on the coast of the Japan Sea, with a light water-moderated reactor of 250 - 300 MW(e) capacity. The construction plan i s currently being pushed forward for completion in 1970. Thereafter three private utility companies - Tokyo, Kansai and Chubu Electric Companies - are doing preparatory work for

Oxidative stress provokes distinct transcriptional responses in the stress-tolerant atr7 and stress-sensitive loh2 Arabidopsis thaliana mutants as revealed by multi-parallel quantitative real-time PCR analysis of ROS marker and antioxidant genes.

Science.gov (United States)

Mehterov, Nikolay; Balazadeh, Salma; Hille, Jacques; Toneva, Valentina; Mueller-Roeber, Bernd; Gechev, Tsanko

2012-10-01

The Arabidopsis thaliana atr7 mutant is tolerant to oxidative stress induced by paraquat (PQ) or the catalase inhibitor aminotriazole (AT), while its original background loh2 and wild-type plants are sensitive. Both, AT and PQ, which stimulate the intracellular formation of H₂O₂ or superoxide anions, respectively, trigger cell death in loh2 but do not lead to visible damage in atr7. To study gene expression during oxidative stress and ROS-induced programmed cell death, two platforms for multi-parallel quantitative real-time PCR (qRT-PCR) analysis of 217 antioxidant and 180 ROS marker genes were employed. The qRT-PCR analyses revealed AT- and PQ-induced expression of many ROS-responsive genes mainly in loh2, confirming that an oxidative burst plays a role in the activation of the cell death in this mutant. Some of the genes were specifically regulated by either AT or PQ, serving as markers for particular types of ROS. Genes significantly induced by both AT and PQ in loh2 included transcription factors (ANAC042/JUB1, ANAC102, DREB19, HSFA2, RRTF1, ZAT10, ZAT12, ethylene-responsive factors), signaling compounds, ferritins, alternative oxidases, and antioxidant enzymes. Many of these genes were upregulated in atr7 compared to loh2 under non-stress conditions at the first time point, indicating that higher basal levels of ROS and higher antioxidant capacity in atr7 are responsible for the enhanced tolerance to oxidative stress and suggesting a possible tolerance against multiple stresses of this mutant. Copyright © 2012 Elsevier Masson SAS. All rights reserved.

Analysis code for pressure in reactor containment vessel of ATR. CONPOL

International Nuclear Information System (INIS)

1997-08-01

For the evaluation of the pressure and temperature in containment vessels in the events which are classified in the abnormal change of pressure, atmosphere and others in reactor containment vessels in accident among the safety evaluation events of the ATR, the analysis code for the pressure in reactor containment vessels CONPOL is used. In this report, the functions of the analysis code and the analysis model are shown. By using this analysis code, the rise of the pressure and temperature in a containment vessel is evaluated when loss of coolant accident occurs, and high temperature, high pressure coolant flows into it. This code possesses the functions of computing blow-down quantity and heat dissipation from reactor cooling facility, steam condensing heat transfer to containment vessel walls, and the cooling effect by containment vessel spray system. As for the analysis techniques, the models of reactor cooling system, containment vessel and steam discharge pool, and the computation models for the pressure and temperature in containment vessels, wall surface temperature, condensing heat transfer, spray condensation and blow-down are explained. The experimental analysis of the evaluation of the pressure and temperature in containment vessels at the time of loss of coolant accident is reported. (K.I.)

Nuclear magnetic resonance (1.40 T) and mid infrared (FTIR-ATR) associated with chemometrics as analytical methods for the analysis of methyl ester yield obtained by esterification reaction

Energy Technology Data Exchange (ETDEWEB)

Kollar, Sara R.M.; Suarez, Paulo A.Z., E-mail: psuarez@unb.br [Universidade de Brasilia (UnB), Brasília, DF (Brazil). Instituto de Química; Novotny, Etelvino H. [Embrapa Solos, Rio de Janeiro, RJ (Brazil); Nascimento, Claudia J. do [Universidade Federal do Estado do Rio de Janeiro (UNIRIO), Rio de Janeiro, RJ, (Brazil). Instituto de Biociências

2017-07-01

In this work, we compared 1.40 T nuclear magnetic resonance (NMR) to 7.05 T (60 and 300 MHz for proton, respectively), and mid-infrared with attenuated total reflectance (FTIR-ATR), associated with chemometrics methods, for the quantification of the reaction yield during esterification of fatty acids with methanol. The results showed that the integrated intensities of the ester C=O stretching region, relative to the total C=O stretching region, is useful to quantify the fatty acid methyl ester (FAME) concentration. Comparing the results obtained by the different final models: NMR (1.40 T and 7.05 T), FTIR-ATR using multivariate partial last squares regression (PLS) with orthogonal signal correction (OSC), and univariate ordinary least squares (OLS), the NMR of 1.40 T (60 MHz for proton) showed more advantages when compared to a high field spectrometer, due to the non-use of cryogenic and solvents and less laborious work for obtaining results. (author)

Japan mellem øst og vest

DEFF Research Database (Denmark)

Hansen, Annette Skovsted; Ipsen, Lene

Skildrer Japans historie gennem de sidste 200 år inklusiv et kapitel om historiebrug ift. Nanjingmassakren i 1937-1938.......Skildrer Japans historie gennem de sidste 200 år inklusiv et kapitel om historiebrug ift. Nanjingmassakren i 1937-1938....

Japan/India. Towards a nuclear cooperation?

International Nuclear Information System (INIS)

Pajon, Celine

2011-10-01

As diplomatic, economic and strategic relationships between Japan and India have been intensively developed for a decade, the author aims at discussing the very sensitive approach to a nuclear cooperation between these two countries as Japan, while taking benefit of the American nuclear umbrella, is a strong defender of nuclear disarmament and non proliferation, and India has been developing its own civilian and military nuclear programme outside of the international regime which it considers as discriminative. The author first discusses factors which incited Japan to build up a strategic partnership with India in front of the evolution of the political context, of the powerful upswing of China, and of the new American orientation with respect to Delhi. She comments the economic and political stakes of the currently negotiated Japan-India nuclear cooperation agreement which not only concerns the relationships between these both countries, but also French and American industrial groups which are present on the Indian market. She also notices that the Fukushima accident which has put Japan energy choices into question again, is a new deal which is to be taken into account

Neurosurgeons in Japan Are Exclusively Brain Surgeons.

Science.gov (United States)

Asamoto, Shunji

2017-03-01

In Japan, neurosurgeons have traditionally mainly treated brain diseases, with most cases involving the spine and spinal diseases historically being treated by orthopedists. Nowadays, spinal surgery is 1 of the many subspecialties in the neurosurgical field in Japan. Most patients with neurological deficits or suspected neurological diseases see board-certified neurosurgeons directly in Japan, not through referrals from family physicians or specialists in other fields. Problems originating in the spine and spinal cord have been overlooked or misdiagnosed in these situations. Neurosurgeons in Japan must rethink the educational program to include advanced trauma life support and spinal surgery. Copyright © 2016. Published by Elsevier Inc.

Evaluation of Corrosion of the Dummy “EE” Plate 19 in YA Type ATR Fuel Element During Reactor PALM Cycles

Energy Technology Data Exchange (ETDEWEB)

Brower, Jeffrey Owen [Idaho National Lab. (INL), Idaho Falls, ID (United States); Glazoff, Michael Vasily [Idaho National Lab. (INL), Idaho Falls, ID (United States); Eiden, Thomas John [Idaho National Lab. (INL), Idaho Falls, ID (United States); Rezvoi, Aleksey Victor [Idaho National Lab. (INL), Idaho Falls, ID (United States)

2016-08-01

Advanced Test Reactor (ATR) Cycle 153B-1 was a 14-day, high-power, powered axial locator mechanism (PALM) operating cycle that completed on April 12, 2013. Cycle 153B-1 was a typical operating cycle for the ATR and did not result in any unusual plant transients. ATR was started up and shut down as scheduled. The PALM drive physically moves the selected experiments into and out of the core to simulate reactor startup and heat up, and shutdown and cooldown transients, while the reactor remains in steady state conditions. However, after the cycle was over, several thousand of the flow-assisted corrosion pits and “horseshoeing” defects were readily observable on the surface of the several YA-type fuel elements (these are “dummy” plates that contain no fuel). In order understand these corrosion phenomena a thermal-hydraulic model of coolant channel 20 on a YA-M fuel element was generated. The boundaries of the model were the aluminum EE plate of a YA-M fuel element and a beryllium reflector block with 13 horizontal saw cuts which represented regions of zero flow. The heat generated in fuel plates 1 through 18 was modeled to be passing through the aluminum EE plate. The coolant channel 20 width was set at 0.058 in. (58 mils). It was established that the horizontal saw cuts had a significant effect on the temperature of the coolant. The flow, which was expected to vary linearly with gradual heating of the coolant as it passed through the channel, was extremely turbulent. The temperature rise, which was expected to be a smooth “S” curve, was represented by a series temperature rise “humps,” which occurred at each horizontal saw cut in the beryllium reflector block. Each of the 13 saw cuts had a chamfered edge which resulted in the coolant flow being re-directed as a jet across the coolant channel into the surface of the EE plate, which explained the temperature rise and the observed sscalloping and possibly pitting degradation on the YA-M fuel elements. In

Mobile Marketing in Japan

OpenAIRE

Noah H. N. Lynn; Paul D. Berger

2014-01-01

In this paper we describe the state of mobile marketing in Japan. We consider the various aspects of mobile marketing in Japan and what has led to the overwhelming adoption by Japanese youth, and to a degree Japanese society as a whole, of social media and associated activities. This growth of mobile marketing has dramatic, positive implications for marketing, in general, as well as for the sale of selected product classes. We also consider markers for suggesting what the future of mobile mar...

Progress on development of nuclear power in Japan

International Nuclear Information System (INIS)

Anon.

2000-01-01

Since three Laws on the nuclear power were published 45 years has passed. Now, development on nuclear power in Japan is at an emergent state. In Japan, 51 units of commercial nuclear reactors with 44.917 GW are in operation, occupy about 37% of total electric power generation, and is positioned at an essential basic energy source supporting economical society in Japan. However, an accident occurred at Tokai Works of the JCO Co., Ltd., one of the uranium reconversion company, on September 30, 1999, was the first critical accident in Japan, and became the worst case in history on development of nuclear power in Japan, because of forming three heavy radiation disabled persons (One of them was dead) in its operators. This was a big crisis with relation to existence on development of nuclear power in Japan, by which anxiety and distrust of the Japanese against the nuclear power were amplified rapidly. On the other side, for Japan short in energy sources and of a big energy consumption, in order to intend for a long term to carry out energy security, global environmental conservation, and sustainable maintenance of essential growth, it remains to be one of important optional methods to further promote nuclear power generation and to establish nuclear fuel cycle. Here were described on progress on peaceful applications of nuclear power in Japan, progress on the field of nuclear power in Japan (from 1955 to 1999), progress on Tokai nuclear power station, introduction of nuclear power generation and effort on its domestic production. (G.K.)

75 FR 67100 - Superalloy Degassed Chromium From Japan

Science.gov (United States)

2010-11-01

... Chromium From Japan AGENCY: United States International Trade Commission. ACTION: Institution of a five-year review concerning the antidumping duty order on superalloy degassed chromium from Japan. SUMMARY... order on superalloy degassed chromium from Japan would be likely to lead to continuation or recurrence...

Analysis code for medium and small rupture accidents in ATR. LOTRAC/HEATUP

International Nuclear Information System (INIS)

1997-08-01

In the evaluation of thermo-hydraulic and fuel temperature transient changes in the events which are classified in medium and small rupture accidents of reactor coolant loss that is the safety evaluation event of the ATR, the analysis code for synthetic thermo-hydraulic transient change at the time of medium and small ruptures LOTRAC and the detailed analysis code for fuel temperature HEATUP are used, respectively. By using the LOTAC, the thermo-hydraulic behavior of reactor cooling facility and the temperature behavior of fuel at the time of blow-down are analyzed, and also the characteristics of changing reactor thermal output is analyzed, considering the functioning characteristics of emergency core cooling system. Based on the data of thermo-hydraulic behavior obtained by the LOTRAC, the time of beginning the turn-around of fuel cladding tube temperature obtained by the data of ECCS pouring characteristics, the heat transfer rate after the turn-around and so on, the detailed temperature change of fuel elements is analyzed by the HEATUP, and the highest temperature and the amount of oxidation of fuel cladding tubes are determined. The LOTRAC code, the HEATUP code, various analysis models, and rupture simulation experiment are reported. (K.I.)

Computer analysis of ATR-FTIR spectra of paint samples for forensic purposes

Science.gov (United States)

Szafarska, Małgorzata; Woźniakiewicz, Michał; Pilch, Mariusz; Zięba-Palus, Janina; Kościelniak, Paweł

2009-04-01

A method of subtraction and normalization of IR spectra (MSN-IR) was developed and successfully applied to extract mathematically the pure paint spectrum from the spectrum of paint coat on different bases, both acquired by the Attenuated Total Reflectance Fourier Transform Infrared (ATR-FTIR) technique. The method consists of several stages encompassing several normalization and subtraction processes. The similarity of the spectrum obtained with the reference spectrum was estimated by means of the normalized Manhattan distance. The utility and performance of the method proposed were tested by examination of five different paints sprayed on plastic (polyester) foil and on fabric materials (cotton). It was found that the numerical algorithm applied is able - in contrast to other mathematical approaches conventionally used for the same aim - to reconstruct a pure paint IR spectrum effectively without a loss of chemical information provided. The approach allows the physical separation of a paint from a base to be avoided, hence a time and work-load of analysis to be considerably reduced. The results obtained prove that the method can be considered as a useful tool which can be applied to forensic purposes.

A Neural Network Controller New Methodology for the ATR-42 Morphing Wing Actuation

Directory of Open Access Journals (Sweden)

Abdallah Ben MOSBAH

2016-06-01

Full Text Available A morphing wing model is used to improve aircraft performance. To obtain the desired airfoils, electrical actuators are used, which are installed inside of the wing to morph its upper surface in order to obtain its desired shape. In order to achieve this objective, a robust position controller is needed. In this research, a design and test validation of a controller based on neural networks is presented. This controller was composed by a position controller and a current controller to manage the current consumed by the electrical actuators to obtain its desired displacement. The model was tested and validated using simulation and experimental tests. The results obtained with the proposed controller were compared to the results given by the PID controller. Wind tunnel tests were conducted in the Price-Païdoussis Wind Tunnel at the LARCASE laboratory in order to calculate the pressure coefficient distribution on an ATR-42 morphing wing model for different flow conditions. The pressure coefficients obtained experimentally were compared with their numerical values given by XFoil software.

Status Summary of FY16 Atom Probe Tomography Studies on UCSB ATR-2 Irradiated RPV Steels

Energy Technology Data Exchange (ETDEWEB)

Wells, Peter [Idaho National Lab. (INL), Idaho Falls, ID (United States); Odette, G. Robert [Idaho National Lab. (INL), Idaho Falls, ID (United States)

2016-05-01

The University of California Santa Barbara-2 RPV Steel Irradiation experiment was awarded in 2010 by the Nuclear Science User Facility (formerly ATR NSUF) through a competitive peer review proposal process. The experiment involved irradiation of nearly 1300 samples distributed over 13 capsules. The major objective of this experiment was to better understand embrittlement behavior of reactor pressure steels at doses beyond which available data exists yet may be achieved if reactor operating licenses are extended beyond 60 years. The experiment was instrumented during irradiation and active temperature control was used to maintain the temperature at the design temperature. Six samples were selected from a large matrix of materials to perform atom probe tomography (APT) to look at formation of high dose phases. The nature and formation behavior of these phases is discussed.

A Cadmium Anionic 1-D Coordination Polymer {[Cd(H2O6][Cd2(atr2(μ2-btc2(H2O4] 2H2O}n within a 3-D Supramolecular Charge-Assisted Hydrogen-Bonded and π-Stacking Network

Directory of Open Access Journals (Sweden)

Anas Tahli

2016-03-01

Full Text Available The hydrothermal reaction of 4,4′-bis(1,2,4-triazol-4-yl (btr and benzene-1,3,5-tricarboxylic acid (H3btc with Cd(OAc2·2H2O at 125 °C in situ forms 4-amino-1,2,4-triazole (atr from btr, which crystallizes to a mixed-ligand, poly-anionic chain of [Cd2(atr2(µ2-btc2(H2O4]2–. Together with a hexaaquacadmium(II cation and water molecules the anionic coordination-polymeric forms a 3-D supramolecular network of hexaaquacadmium(II-catena-[bis(4-amino-1,2,4-triazoletetraaquabis(benzene-1,3,5-tricarboxylatodicadmate(II] dihydrate, 1-D-{[Cd(H2O6][Cd2(atr2(µ2-btc2(H2O4] 2H2O}n which is based on hydrogen bonds (in part charge-assisted and π–π interactions.

Universal varicella vaccine immunization in Japan.

Science.gov (United States)

Yoshikawa, Tetsushi; Kawamura, Yoshiki; Ohashi, Masahiro

2016-04-07

In 1974, Japanese scientists developed a live attenuated varicella vaccine based on the Oka strain. The efficacy of the vaccine for the prevention of varicella has been primarily demonstrated in studies conducted in the United States following the adoption of universal immunization using the Oka strain varicella vaccine in 1996. Although the vaccine was developed by Japanese scientists, until recently, the vaccine has been administered on a voluntary basis in Japan resulting in a vaccine coverage rate of approximately 40%. Therefore, Japan initiated universal immunization using the Oka strain varicella vaccine in November 2014. Given the transition from voluntary to universal immunization in Japan, it will also be important to monitor the epidemiology of varicella and herpes zoster. The efficacy and safety of co-administration of the varicella vaccine and measles, mumps, and rubella vaccine have been demonstrated in many countries; however, there was no data from Japan. In order to adopt the practice of universal immunization using the Oka strain varicella vaccine in Japan, data demonstrating the efficacy and safety of co-administration of varicella vaccine and measles and rubella (MR) vaccine were required. Additionally, we needed to elucidate the appropriate time interval between the first and second administrations of the vaccine. It is also important to differentiate between wild type and Oka vaccine type strains in herpes zoster patient with past history of varicella vaccine. Thus, there are many factors to consider regarding the adoption of universal immunization in Japan to control varicella zoster virus (VZV) infections. Copyright © 2016 Elsevier Ltd. All rights reserved.

Modulation of the DNA repair system and ATR-p53 mediated apoptosis is relevant for tributyltin-induced genotoxic effects in human hepatoma G2 cells.

Science.gov (United States)

Li, Bowen; Sun, Lingbin; Cai, Jiali; Wang, Chonggang; Wang, Mengmeng; Qiu, Huiling; Zuo, Zhenghong

2015-01-01

The toxic effects of tributyltin (TBT) have been extensively documented in several types of cells, but the molecular mechanisms related to the genotoxic effects of TBT have still not been fully elucidated. Our study showed that exposure of human hepatoma G2 cells to 1-4 μmol/L TBT for 3 hr caused severe DNA damage in a concentration-dependent manner. Moreover, the expression levels of key DNA damage sensor genes such as the replication factor C, proliferating cell nuclear antigen and poly (ADP-ribose) polymerase-1 were inhabited in a concentration-dependent manner. We further demonstrated that TBT induced cell apoptosis via the p53-mediated pathway, which was most likely activated by the ataxia telangiectasia mutated and rad-3 related (ATR) protein kinase. The results also showed that cytochrome c, caspase-3, caspase-8, caspase-9, and the B-cell lymphoma 2 were involved in this process. Taken together, we demonstrated for the first time that the inhibition of the DNA repair system might be more responsible for TBT-induced genotoxic effects in cells. Then the generated DNA damage induced by TBT initiated ATR-p53-mediated apoptosis. Copyright © 2014. Published by Elsevier B.V.

Business Ownership and Unemployment in Japan

NARCIS (Netherlands)

J. van Acht; J. Stam; A.R. Thurik (Roy); I. Verheul (Ingrid)

2004-01-01

textabstractThe influence of industrial structure, more specifically of business ownership, is investigated on the level of unemployment in Japan. The question is to what extent business ownership, i.e., entrepreneurship, can reduce the level of unemployment. It will be concluded that Japan is

Information on research in progress in Japan publication of 'Nuclear Science Information of Japan-Oral Presentation'

Energy Technology Data Exchange (ETDEWEB)

Itabashi, Keizo; Nakajima, Hidemitsu; Yokoo, Hiroshi

1988-03-01

The new journal, 'Nuclear Science Information of Japan-Oral Presentation', which was entirely revised from the previous abstract journal called 'Nuclear Science Information of Japan' was introduced. This is to be published quaterly in principle and compiled information by oral presentation presented at main conferences, symposia and other kind of formal meetings in the field of nuclear science and technology in Japan. Not all of the oral presentation is always contributed later to a proceeding or a journal as a full paper in Japan. In some cases, the pre-conference paper might be a only publication of the oral presentation. In this meaning, this journal could be used as a search tool for the subjects and the projects of nuclear research and development in progress.

From sword to chrysanthemum: Japan's culture of anti-miltarism

International Nuclear Information System (INIS)

Berger, T.U.

1993-01-01

The end of the Cold War and the phenomenal increase in Japan's economic and technological power put Japan today in the position to become, if it chooses, a military as well as economic superpower. The diminution of the Soviet threat and the increasing US preoccupation with domestic problems give Japan a latitude for independent action it has not had since the end of World War II. At the same time the US-Japanese security alliance, which has enabled Japan to adopt a minimalist approach to defense and national security, is being weakened by ideologically charged trade and other economic frictions and a growing American perception of Japan as a threat to its interests. Moreover, in the long run Japan faces the prospect of having to deal with other rising regional powers, most notably the People's Republic of China. This changing international security environment thus raises question whether Japan, having become an economic rival of the United States, may not in the future become a military competitor as well; whether, after having adopted a pacifist stance for half a century, Japan may choose to unsheathe its sword once again

U.S.-Japan Quake Prediction Research

Science.gov (United States)

Kisslinger, Carl; Mikumo, Takeshi; Kanamori, Hiroo

For the seventh time since 1964, a seminar on earthquake prediction has been convened under the U.S.-Japan Cooperation in Science Program. The purpose of the seminar was to provide an opportunity for researchers from the two countries to share recent progress and future plans in the continuing effort to develop the scientific basis for predicting earthquakes and practical means for implementing prediction technology as it emerges. Thirty-six contributors, 15 from Japan and 21 from the U.S., met in Morro Bay, Calif.September 12-14. The following day they traveled to nearby sections of the San Andreas fault, including the site of the Parkfield prediction experiment. The conveners of the seminar were Hiroo Kanamori, Seismological Laboratory, California Institute of Technology (Caltech), for the U.S., and Takeshi Mikumo, Disaster Prevention Research Institute, Kyoto University, for Japan . Funding for the participants came from the U.S. National Science Foundation and the Japan Society forthe Promotion of Science, supplemented by other agencies in both countries.

Blood glucose measurement with multiple quantum cascade lasers using hollow-optical fiber-based ATR spectroscopy

Science.gov (United States)

Yoshioka, K.; Kino, S.; Matsuura, Y.

2018-02-01

For non-invasive blood glucose measurement, a measurement system based on mid-infrared ATR spectroscopy equipped with a combination of a QCL as a light source and a hollow-optical fiber as a beam delivery medium is developed. Firstly the measurement sensitivity of the system is evaluated by using glucose solutions and the result shows a significant correlation between optical absorbance and solution concentration. It is also confirmed that the system has a sensitivity that is enough for blood glucose measurement. Then optical absorption of human lips in the mid-infrared wavelength region is measured using a QCL with a wavenumber of 1080 cm-1 where human tissue exhibits strong absorption of glucose and its metabolites. As a result, the measured absorption follows the change of blood glucose well with a time delay of around 10 minutes and correlation factor between the absorbance and the blood glucose level is 0.42.

PLS models for determination of SARA analysis of Colombian vacuum residues and molecular distillation fractions using MIR-ATR

Directory of Open Access Journals (Sweden)

Jorge A. Orrego-Ruiz

2014-06-01

Full Text Available In this work, prediction models of Saturates, Aromatics, Resins and Asphaltenes fractions (SARA from thirty-seven vacuum residues of representative Colombian crudes and eighteen fractions of molecular distillation process were obtained. Mid-Infrared (MIR Attenuated Total Reflection (ATR spectroscopy in combination with partial least squares (PLS regression analysis was used to estimate accurately SARA analysis in these kind of samples. Calibration coefficients of prediction models were for saturates, aromatics, resins and asphaltenes fractions, 0.99, 0.96, 0.97 and 0.99, respectively. This methodology permits to control the molecular distillation process since small differences in chemical composition can be detected. Total time elapsed to give the SARA analysis per sample is 10 minutes.

Fast and calibration free determination of first order reaction kinetics in API synthesis using in-situ ATR-FTIR.

Science.gov (United States)

Rehbein, Moritz C; Husmann, Sascha; Lechner, Christian; Kunick, Conrad; Scholl, Stephan

2018-05-01

In early stages of drug development only sparse amounts of the key substances are available, which is problematic for the determination of important process data like reaction kinetics. Therefore, it is important to perform experiments as economically as possible, especially in regards to limiting compounds. Here we demonstrate the use of a temperature step experiment enabling the determination of complete reaction kinetics in a single non-isothermal experiment. In contrast to the traditionally used HPLC, the method takes advantage of the high measuring rate and the low amount of labor involved in using in-situ ATR-FTIR to determine time-dependent concentration-equivalent data. Copyright © 2017 Elsevier B.V. All rights reserved.

Japan-U.S. Relations: Issues for Congress

Science.gov (United States)

2008-05-23

fields in an area of the East China Sea that both countries claim as their territory. Hu also announced that China would lease two giant pandas to Japan...to replace a recently deceased panda at a Tokyo zoo. Days later, after China was struck by a devastating earthquake, Japan immediately offered...other shipments of U.S. beef from Japan. In May 2007, the World Organization for Animal Health (OIE) announced that the United States was a “controlled

Understanding Great Earthquakes in Japan's Kanto Region

Science.gov (United States)

Kobayashi, Reiji; Curewitz, Daniel

2008-10-01

Third International Workshop on the Kanto Asperity Project; Chiba, Japan, 16-19 February 2008; The 1703 (Genroku) and 1923 (Taisho) earthquakes in Japan's Kanto region (M 8.2 and M 7.9, respectively) caused severe damage in the Tokyo metropolitan area. These great earthquakes occurred along the Sagami Trough, where the Philippine Sea slab is subducting beneath Japan. Historical records, paleoseismological research, and geophysical/geodetic monitoring in the region indicate that such great earthquakes will repeat in the future.

Partnership between Japan and North Atlantic treaty organization

Directory of Open Access Journals (Sweden)

Budimir Željko

2016-01-01

Full Text Available In the paper, the relationship between Japan and the North Atlantic Alliance, the development of their partnership and Japan's contribution to the NATO missions throughout the world, as well as the current status that Japan has in the Alliance, are described and explained. The authors of the paper have presented and explained the models of cooperation between the two sides and the perspectives of their future relationships as well. Apart from the said, the internal political and legal transformation of Japan, directed towards the country's greater and more robust engagement in international missions, including the use of force, which has carefully been avoided in the past seven decades due to constitutional and legal limitations, is also shown in parallel with that. The underlying thesis statement of the paper reads as follows: Japan's coming closer to NATO, i.e. the strengthening of their partnership and the elimination of legal obstacles in Japan itself to the use of force in international politics, are complementary processes. In the paper, the analytical, synthetic and comparative methods are used.

The photovoltaic energy in Japan

International Nuclear Information System (INIS)

Georgel, O.

2005-07-01

Today the Japan is the leader of the photovoltaic energy. The first reason of this success is an action of the government integrating subventions for the installation of photovoltaic systems and a support of the scientific research. To explain this success, the author presents the energy situation in Japan, details the national programs, the industrial sector (market, silicon needs, recycling, manufacturers, building industry) and presents the main actors. (A.L.B.)

Proto-Industrialization in Tokugawa Japan

OpenAIRE

Murphy, Martin J.

2012-01-01

This research examines the process of early capitalism in Japanese history. Generally it is thought that capitalism developed in the years leading to the Meiji Restoration. Like European models of early capitalist development in handicraft and cottage industries, Japan went through a similar process. Development of rural trade and small-scale capitalism sowed the seeds that broke feudal bonds and resulted in the mass factory system that made modern capitalist Japan.

Tratamiento del maxilar posterior atrófico mediante técnica de reconstrucción tridimensional con elevación de seno y abordaje «en tunel»

Directory of Open Access Journals (Sweden)

Andrés Restoy

2015-01-01

Conclusiones: El tratamiento del sector posterior maxilar atrófico mediante reconstrucción tridimensional con autoinjerto óseo, elevación sinusal y abordaje por tunelización es una técnica que proporciona resultados predecibles y estables, permitiendo la rehabilitación con coronas sobre implantes dentales de dimensiones adecuadas.

Radical external beam radiotherapy for prostate cancer in Japan. Differences in the patterns of care between Japan and the United States

International Nuclear Information System (INIS)

Ogawa, Kazuhiko; Nakamura, Katsumasa; Sasaki, Tomonari; Onishi, Hiroshi; Araya, Masayuki; Koizumi, Masahiko; Okamoto, Atsushi; Tejima, Teruki; Mitsumori, Michihide

2007-01-01

This study focused on the differences in the patterns of care for prostate cancer patients treated with radical external beam radiotherapy between Japan and the United States. Results from the 1999-2001 Japanese Patterns of Care Study (PCS) survey were compared with those of the 1999 PCS in the United States. In addition, the changing trends in the patterns of care between Japan and the United States were also analyzed. Patients in Japan were found to have more advanced primary disease than those in the United States, but the proportions of advanced disease have gradually decreased in Japan. The distributions of CT-based treatment planning, conformal therapy and higher doses were higher in the United States, and a drastic change in these parameters occurred in the United States, while only moderate changes occurred in Japan. These results indicate that patterns of care for prostate cancer in Japan are considerably different from those in the United States, and the changing trends in the patterns of care are also different between the two countries. (author)

Organic photovoltaic energy in Japan

International Nuclear Information System (INIS)

2007-01-01

Japan finances research programs on photovoltaic conversion since 1974. Research in this domain is one of the 11 priorities of NEDO, the agency of means of the ministry of economy, trade and industry of Japan. The search for an abatement of production costs and of an increase of cells efficiency is mentioned in NEDO's programs as soon as the beginning of the 1990's. A road map has been defined which foresees photovoltaic energy production costs equivalent to the ones of thermal conversion by 2030, i.e. 7 yen/kWh (4.4 cents of euro/kWh). The use of new materials in dye-sensitized solar cells (DSSC) or organic solar cells, and of new structures (multi-junctions) is explored to reach this objective. The organic photovoltaic technology is more particularly considered for small generation units in mobile or domestic technologies. Japan is particularly in advance in the improvement of DSSC cells efficiency, in particular in the domain of the research on solid electrolytes. Europe seems more in advance in the domain of the new generation of organic solar cells. Therefore, a complementarity may be found between Japan and French teams in the domain of organic solar cells improvement through collaboration programs. (J.S.)

Japan 2014

DEFF Research Database (Denmark)

Selmer, Finn

2014-01-01

In March 2014 a group of teachers and students from the Royal Danish Academy of Fine Arts Schools of Architecture, Design and Conservation, School of Architecture, Study Dept. 3 went on a study trip to Japan. This publication collects observations and reflections that the participants of the trip...

Male acceptance of condoms in Japan.

Science.gov (United States)

Uchida, Y

1981-01-01

Current surveys indicate that more than 70% of married couples in Japan use condoms as their primary contraceptive method. The popularity of condoms is due to the oral contraceptive (OC) pills not being recognized as safe forms of contraception and IUDs not being legalized by the government until 1974. The history of condom use in Japan goes back to 1872 when condoms of thin leather were imported into Japan from England and France. Manufacturing of condoms in Japan began in 1909, mainly as a method for venereal disease prevention. The condom and induced abortion are now the major methods of contraception. 60% of the distribution of condoms is through pharmacies and cosmetic stores; 50% of the buyers are women. Since 1955, teams of family planning workers have distributed condoms by selling on a door-to-door basis, especially to lower middle class couples. Between 1969-75, 75% of contraceptive users were using condoms. Currently there are 81% users. The rhythm method ranks second in popularity at 30% between 1969-75. OCs have gradually increased recently to 3% users, but side effects have deterred people from selecting them. The proportion of IUD users has remained at a consistently low level for the past several years. Japan accounts for 1/3 of the total world production of condoms.

L-Band RFI in Japan

Science.gov (United States)

Soldo, Yan; de Matthaeis, Paolo; Le Vine, David M.

2016-01-01

In recent years, three instruments have been launched into orbit with the aim of producing global maps of sea surface salinity and soil moisture using the 1400-1427 MHz band: SMOS, Aquarius and SMAP. Although this frequency band is allocated to passive measurements only, RFI (Radio-Frequency Interference) is present in the data of all three missions. On a global scale, the three sensors have observed approximately the same distribution of RFI. Japan is an important exception that has implications for the design of RFI detection algorithms. RFI in Japan is caused by a large number of emitters belonging to the same system (TV receivers) and for this reason some traditional RFI detection strategies detect little to no RFI over Japan. The study of this case has led to an improvement of the approach to detect RFI in Aquarius data.

An overview of work, retirement, and pensions in Japan.

Science.gov (United States)

Bass, S A

1996-01-01

Work to retirement in Japan is a sequential transition for the most part, and Japan permits mandatory retirement by firms at age 60. But many older people work beyond the age of 60, many more than in other industrialized countries. A number of hypotheses are examined, having to do with pensions, health, opportunity, interest in working, cultural attitudes (including the concept of ikigai), and public policy initiatives (such as employment policy and the Silver Human Resource Centers). Japan's cultural attitudes and existing policies appear to have set Japan on a unique course in considering the aging of its population. To what extent should other nations emulate Japan?

Japan's oil situation and relationship with the Middle East

International Nuclear Information System (INIS)

Oka, H.

1991-01-01

This paper reports on Japan's energy supply structure and the demand for energy in the country. It focuses on oil relations between Japan and the Middle Eastern oil countries, and Japan's attempt to enhance energy supply-security policy measures

History and structure of Japan-US nuclear alliance

International Nuclear Information System (INIS)

Yoshioka, Hitoshi

2011-01-01

'Japan-US nuclear alliance' for civil use of nuclear energy was used here as technical term for the state Japanese commercial power plants were all water-cooled reactors under the US nuclear engineering umbrella and US admitted Japanese development of all 'SNT: sensitive nuclear technology' in the area of nuclear fuel cycle. 'Japan-US alliance' was used for various areas closely related with national security while 'Japan-US nuclear weapon alliance' was for military use of nuclear energy such as nuclear weapon (transport methods included) and counter weapon system. Military and civil use of nuclear energy relied fundamentally on common technical bases and especially nuclear fuel cycle related technologies were directly connected to both uses and called 'SNT'. Japanese nuclear policy sticking to SNT might come from the axiom: nuclear engineering for national security, that meant Japan refrained from nuclear arms but maintained technical and industrial potential of nuclear arms. This could be called 'nuclear arms standby strategy' and derived from compromise of both countries to play role of stabilizer of 'Japan-US alliance'. History of Japanese nuclear power development could be well understood as formation process of 'Japan-US nuclear alliance'. If Fukushima Daiichi accident forced nuclear power to phase out, nuclear fuel cycle would be obliged to terminate. This meant failure of the axiom and dissolution of 'Japan-US nuclear alliance'. (T. Tanaka)

Japan Sports Arbitration Agency (JSAA

Directory of Open Access Journals (Sweden)

Ekaterina P. Rusakova

2015-12-01

Full Text Available In this article author analyzes the activities of Japan Sports Arbitration Agency. Author considers the goals, objectives and procedure for dealing with disputes relating to the use of performance-enhancing drugs by athletes. Author study the regulation of Japan Sports Arbitration Agency, to resolve disputes relating to the use of doping, as well as the procedure for application and acceptance of its agency, the choice of arbitrators, counterclaim, protection of evidence.

The IAEA's Activities in a Changing World, Foreign Correspondent's Club of Japan, 17 March 2014, Tokyo, Japan

International Nuclear Information System (INIS)

Amano, Y.

2014-01-01

The last time I had the pleasure of speaking to the Foreign Correspondents' Club of Japan was in December 2010. I had been IAEA Director General for about a year. Three months later came the Fukushima Daiichi accident. It was triggered by a powerful tsunami which followed the Great East Japan Earthquake. Human and organizational failings also played a part. Helping Japan deal with the aftermath of the accident has been a top priority for the IAEA, and for me personally, in the past three years. Nuclear safety is a hugely important issue, both in Japan and throughout the world. We have been working with our 162 Member States to implement the IAEA Action Plan on Nuclear Safety, which was adopted soon after the accident. Just recently, the final reports of our international review missions on decommissioning, and on remediation of large contaminated off-site areas, were made public. Both missions observed good progress in their respective areas. The IAEA is preparing a report on the Fukushima Daiichi accident, which will be finalized by the end of this year and shared with our Member States next year. I welcome the way in which Japan is sharing its post-Fukushima experience with the rest of the world and I encourage all States to make full use of IAEA services in order to help raise levels of safety everywhere

Annual report of Power Reactor and Nuclear Fuel Development Corporation, fiscal year 1996

Energy Technology Data Exchange (ETDEWEB)

NONE

1997-09-01

The experimental FBR `Joyo` has continued the irradiation operation at 100 MWt. After the 11th periodic inspection, the 30th cycle operation was carried out. The cumulative operation time as of the end of the fiscal year was 51,630 hours, and the cumulative heat output was about 4.2 billion kWh. The prototype FBR `Monju` has succeeded in electric power generation in August, 1995, but the sodium leak accident occurred in December, 1995. The elucidation of the cause of the sodium leak accident and the total inspection for the safety have been carried out. As for FBRs, the research and development of the reactor physics, the design of a large FBR, the equipment systems, the fuel and materials, the structures and the safety have been advanced. The ATR `Fugen` Power Station has continued the operation smoothly, and as of the end of the fiscal year, the total generated electric power was about 17.3 billion kWh, and the capacity factor was 66.3%. It boasts about the result of using MOX fuel. The exploration of uranium resources, the development of uranium conversion, uranium enrichment and plutonium fuel, the reprocessing of spent fuel, the development of environmental technology for radioactive waste, creative and innovative research and development, safety control and safety research and others are reported. (K.I.)

Japan - IAEA joint Nuclear Energy Management School 2016

International Nuclear Information System (INIS)

Yamaguchi, Mika; Hidaka, Akihide; Ikuta, Yuko; Yamashita, Kiyonobu; Sawai, Tomotsugu; Murakami, Kenta; Uesaka, Mitsuru; Tomita, Akira; Toba, Akio; Hirose, Hiroya; Watanabe, Masanori; Kitabata, Takuya; Ueda, Kinichi; Kita, Tomohiko; Namaizawa, Ken; Onose, Takatoshi

2017-03-01

Since 2010, International Atomic Energy Agency (IAEA) has held the 'Nuclear Energy Management School' so-called 'IAEA-NEM' to develop future leaders who plan and manage nuclear energy utilization in their county. Since 2012, Japan Atomic Energy Agency (JAEA) together with the Japan Nuclear Human Resource Development Network (JN-HRD Net), the University of Tokyo (UT), the Japan Atomic Industrial Forum (JAIF) and JAIF International Cooperation Center (JICC) have cohosted the NEM school in Japan in cooperation with IAEA. Since then, the school has been held every year, with the school in 2016 marking the fifth. In the 2016 NEM school, Japanese nuclear energy technology and experience, such as lessons learned from the Fukushima Daiichi Nuclear Power Station accident, were provided by not only lectures by IAEA experts, but also lectures by Japanese experts and leaders in order to offer a unique opportunity for the participants from other countries to learn about particular cases in Japan. Opportunities to visit a variety of nuclear facilities were offered for the participants in the form of technical tours in Fukui and Kobe. Through the school, we contributed to the internationalization of Japanese young nuclear professionals, development of nuclear human resource of other countries including nuclear newcomers, and enhanced cooperative relationship between IAEA and Japan. Additionally, collaborative relationship with JN-HRD Net was strengthened solidly through the integrated cooperation among ministries, universities, manufacturers and research organizations across the county by holding the school in Japan. In this report, findings obtained during the preparatory work and the school period were reported in order to make a valuable contribution towards effectively and efficiently conducting future international nuclear human resource development activities in Japan. (author)

Establishment of computer aided technology for operation, maintenance, and core management

International Nuclear Information System (INIS)

Iguchi, Masaki; Isomura, Kazutoshi; Okawa, Tsuyoshi; Sakurai, Naoto

2003-01-01

In Fugen, the accumulated know-how of skilled operators, maintenance engineers, and core management engineers have been systematized by using the latest computer technology. These computerized systems have enhanced the technology of operating, maintenance and core management. This report describes the development of a reactor feed water control system with fuzzy logic, a refueling support system, and an automatic refueling planning system. Since operation of reactor feedwater control at low power requires a delicate operational technique and the knowledge and experience of operators, the application of a fuzzy algorithm was deemed effective in Fugen. Its good performance comparable to that of experienced operators can be realized. The fuel-handling operation takes proposed plans, fuel management and efficient operation by skilled operators. AI technology was applied to fuel-handling support system using past operation results and experience of skilled operators. This system is as capable of fuel-handling as skilled operators. Planning an adequate fuel loading pattern is time-consuming even for expert core management engineers. The Automatic Refueling Planning System (ARPS) was developed using Genetic Algorithms (GA) and a Simulated Annealing (SA). It has been verified that long-term fuel loading patterns of the Fugen NPS evaluated by ARPS are equivalent to that of an expert core management engineer. (author)

2012/13 abnormal cold winter in Japan associated with Large-scale Atmospheric Circulation and Local Sea Surface Temperature over the Sea of Japan

Science.gov (United States)

Ando, Y.; Ogi, M.; Tachibana, Y.

2013-12-01

On Japan, wintertime cold wave has social, economic, psychological and political impacts because of the lack of atomic power stations in the era of post Fukushima world. The colder winter is the more electricity is needed. Wintertime weather of Japan and its prediction has come under the world spotlight. The winter of 2012/13 in Japan was abnormally cold, and such a cold winter has persisted for 3 years. Wintertime climate of Japan is governed by some dominant modes of the large-scale atmospheric circulations. Yasunaka and Hanawa (2008) demonstrated that the two dominant modes - Arctic Oscillation (AO) and Western Pacific (WP) pattern - account for about 65% of the interannual variation of the wintertime mean surface air temperature of Japan. A negative AO brings about cold winter in Japan. In addition, a negative WP also brings about cold winter in Japan. Looking back to the winter of 2012/13, both the negative AO and negative WP continued from October through December. If the previous studies were correct, it would have been extremely very cold from October through December. In fact, in December, in accordance with previous studies, it was colder than normal. Contrary to the expectation, in October and November, it was, however, warmer than normal. This discrepancy signifies that an additional hidden circumstance that heats Japan overwhelms these large-scale atmospheric circulations that cool Japan. In this study, we therefore seek an additional cause of wintertime climate of Japan particularly focusing 2012 as well as the AO and WP. We found that anomalously warm oceanic temperature surrounding Japan overwhelmed influences of the AO or WP. Unlike the inland climate, the island climate can be strongly influenced by surrounding ocean temperature, suggesting that large-scale atmospheric patterns alone do not determine the climate of islands. (a) Time series of a 5-day running mean AO index (blue) as defined by Ogi et al., (2004), who called it the SVNAM index. For

A distrofia miotônica ou miotonia atrófica: estudo sintético-crítico

Directory of Open Access Journals (Sweden)

Enzo Azzi

1953-03-01

Full Text Available O autor, baseado em algumas observações clínicas pessoais, individualiza os elementos principais (atróficos, miotônicos, neurendócrino-vegetativos que se referem à distrofia miotônica, insistindo sôbre a riqueza dos sintomas infundíbulo-hipofisários observados (distrofia adiposogenital, policitemia, perturbações do sono, constante hipoplasia da sela turca que falam em favor de uma meiopragia diencéfalo-hipofisária como mecanismo fisiopatogênico da doença. O autor discute também o problema das relações entre distrofia miotônica e miotonia de Thomsen, concluindo, com base em um de seus casos, pela identidade fundamental das duas formas e, portanto, pela sua unificação.

A Simple Approach to Distinguish Classic and Formaldehyde-Free Tannin Based Rigid Foams by ATR FT-IR

Directory of Open Access Journals (Sweden)

Gianluca Tondi

2015-01-01

Full Text Available Tannin based rigid foams (TBRFs have been produced with formaldehyde since 1994. Only recently several methods have been developed in order to produce these foams without using formaldehyde. TBRFs with and without formaldehyde are visually indistinguishable; therefore a method for determining the differences between these foams had to be found. The attenuated total reflectance infrared spectroscopy (ATR FT-IR investigation of the TBRFs presented in this paper allowed discrimination between the formaldehyde-containing (classic and formaldehyde-free TBRFs. The spectra of the formaldehyde-free TBRFs, indeed, present decreased band intensity related to the C–O stretching vibration of (i the methylol groups and (ii the furanic rings. This evidence served to prove the chemical difference between the two TBRFs and explained the slightly higher mechanical properties measured for the classic TBRFs.

Effects of Near Infrared Radiation on DNA. DLS and ATR-FTIR Study

Science.gov (United States)

Szymborska-Małek, Katarzyna; Komorowska, Małgorzata; Gąsior-Głogowska, Marlena

2018-01-01

We presume that the primary effect of Near Infrared (NIR) radiation on aqueous solutions of biological molecules concerns modification of hydrogen bonded structures mainly the global and the hydration shell water molecules. Since water has a significant influence on the DNA structure, we expect that the thermal stability of DNA could be modified by NIR radiation. The herring sperm DNA was exposed to NIR radiation (700-1100 nm) for 5, 10, and 20 min periods. The temperature dependent infrared measurements were done for the thin films formed on the diamond ATR crystal from evaporated DNA solutions exposed and unexposed to NIR radiation. For the NIR-treated samples (at room temperature) the B form was better conserved than in the control sample independently of the irradiation period. Above 50 °C a considerable increase in the A form was only observed for 10 min NIR exposed samples. The hydrodynamic radius, (Rh), studied by the dynamic light scattering, showed drastic decrease with the increasing irradiation time. Principal components analysis (PCA) allowed to detect the spectral features correlated with the NIR effect and thermal stability of the DNA films. Obtained results strongly support the idea that the photoionization of water by NIR radiation in presence of DNA molecules is the main factor influencing on its physicochemical properties.

The cost of schizophrenia in Japan

Directory of Open Access Journals (Sweden)

Sado M

2013-05-01

Full Text Available Mitsuhiro Sado,1 Ataru Inagaki,2 Akihiro Koreki,1 Martin Knapp,3 Lee Andrew Kissane,4 Masaru Mimura,1 Kimio Yoshimura4 1Department of Neuropsychiatry, Keio University School of Medicine, 2Center for Clinical Psychopharmacology, Institute of Neuropsychiatry, Tokyo, Japan; 3Department of Social Policy, London School of Economics and Political Science, London, UK; 4Department of Health Policy and Management, Keio University School of Medicine, Tokyo, Japan Introduction: Schizophrenia is a disorder that produces considerable burdens due to its often relapsing/remitting or chronic longitudinal course. This burden is felt not only by patients themselves, but also by their families and health care systems. Although the societal burden caused by this disorder has been evaluated in several countries, the magnitude of the societal cost of schizophrenia in Japan has never been estimated. The aim of this study is to clarify the societal burden of schizophrenia by estimating the cost of schizophrenia in Japan in 2008. Methods: A human capital approach was adopted to estimate the cost of schizophrenia. The total cost of schizophrenia was calculated as the sum of the direct, morbidity, and mortality costs. Schizophrenia was defined as disorders coded as F20.0–F20.9 according to the International Classification of Diseases-10. The data required to estimate the total cost was collected from publicly available statistics or previously reported studies. Results: The total cost of schizophrenia in Japan in 2008 was JPY 2.77 trillion (USD 23.8 billion. While the direct cost was JPY 0.770 trillion (USD 6.59 billion, the morbidity and mortality costs were JPY 1.85 trillion (USD 15.8 billion and JPY 0.155 trillion (USD 1.33 billion, respectively. Conclusion: The societal burden caused by schizophrenia is tremendous in Japan, similar to that in other developed countries where published data exist. Compared with other disorders, such as depression or anxiety disorders

Japan country report

Energy Technology Data Exchange (ETDEWEB)

Morisaki, Rieko [Energy Communication Planning, 3-9-16 Aobadai, 818-0137 Dazaifu (Japan)

2008-07-01

1. Nuclear 2007 highlights: - A magnitude 6.8 earthquake occurred in Niigata on July 16 2007. Owing to this earthquake, 3 units operating and 1 unit during start-up were shutdown automatically at TEPCO's Kashiwazaki-Kariwa NPS. Now, all 7 units of the NPS are in an outage for investigation. This influenced the capacity factor of Japanese NPPs in FY 2007, which stood at just 60.7%. - Debate on global warming is more and more active in Japan, as it is the host country of the G8 Hokkaido Toyako Summit in July. The Japan Atomic Energy Commission released 'White Paper on Nuclear Energy 2007' in March 2008. In the paper, they first expressed the view that the expansion of the peaceful use of nuclear energy is indispensable. 2. Nuclear overview: a. Energy policy: Electricity share: 25.4% of nuclear. The energy policy of Japan aims at nuclear power generation being maintained at the current level (30 to 40% of the total electricity generation) or increasing even after 2030, for stable energy supply and as a countermeasure against global warming. - Nuclear Fuel Cycle: The active tests at the JNFL reprocessing plant in Rokkasho-mura are in the final phase for commercial operation in 2008. By FY 2010 Plutonium utilization in LWRs in 16 to 18 NPP units. Around FY 2010 Installment of new centrifuges at the uranium enrichment plant at Rokkasho-mura. In FY 2012 Start of commercial operation of MOX fuel fabrication plant. Fast-breeder reactor cycle: Operation of the prototype reactor 'MONJU' has been suspended since a secondary sodium leak in 1995. JAEA (Japan Atomic Energy Agency) completed full-scale remodeling work and is implementing various tests to confirm the capabilities and soundness of MONJU. They aim to start its operation within FY 2008. Around 2025 Building a demonstration FBR. Before 2050 Development of a commercial FBR. - Electricity production (Operating): BWR: 32 units (including 4 units of APWR), PWR: 23 units. - Electricity production

Middle term prospects for Japan's safeguards

International Nuclear Information System (INIS)

Ogawa, T.

2001-01-01

Japan has responded to IAEA requirements on reinforced safeguard regulations. The IAEA additional protocol entered in force in Japan on December 1999. Japan submitted a preliminary information report to IAEA on June 2000 after joint works with the Nuclear Material Control Center (NMCC) of Japan. The first annual report was submitted to IAEA on May 2001. Another activity for the additional protocol is complementary accesses. The total 36 accesses to facilities have been done from November 2000 to September 2001. Procedures of access to managements are under discussion. MEXT (Ministry of Education, Culture, Sports, Science and Technology) has been constructing the Rokkasho Safeguards On-Site Laboratory from 1997, and the Rokkasho Safeguards Center from 2000. The Design Information Verification (DIV) is now ongoing. Much more personal resources will be needed for future inspections. Therefore, the budget for safeguards is increasing in contrast to the flat base budget for the total atomic energy. As for future activity, a MOX (Mixed Oxide Fuels) fuel processing plant is one of the issues for discussion. The construction of the MOX processing plant is supposed to begin on around 2004. The conclusion of additional protocol will be given by IAEA until end of 2002. Shift to integrated safeguards are under discussions by MEXT, NMCC and utilities of Japan parallel with IAEA. Key issues of discussion are cost saving for safeguards, development of personal resources for inspectors and the role of NMCC. (Y. Tanaka)

75 FR 57980 - Polychloroprene Rubber From Japan

Science.gov (United States)

2010-09-23

... INTERNATIONAL TRADE COMMISSION [Investigation No. AA1921-129 (Third Review)] Polychloroprene Rubber From Japan AGENCY: United States International Trade Commission. ACTION: Termination of five-year... of the antidumping duty finding on polychloroprene rubber from Japan would be likely to lead to...

ATR-SEIRAS study of CO adsorption and oxidation on Rh modified Au(111-25 nm) film electrodes in 0.1 M H2SO4

International Nuclear Information System (INIS)

Xu, Qinqin; Berná, Antonio; Pobelov, Ilya V.; Rodes, Antonio; Feliu, Juan M.; Wandlowski, Thomas; Kuzume, Akiyoshi

2015-01-01

Rh modified Au(111-25 nm) electrodes, prepared by electron beam evaporation and galvanostatic deposition, were employed to study adsorption and electro-oxidation of CO on Rh in 0.1 M sulfuric acid solution by in situ attenuated total reflection surface enhanced infrared absorption spectroscopy (ATR-SEIRAS). The results of ATR-SEIRAS experiments were compared with those obtained by infrared reflection absorption spectroscopy on three low-index Rh single crystal surfaces. The Rh film deposited on Au(111-25 nm) electrode consists of 3D clusters forming a highly stepped [n(111) × (111)]-like surface with narrow (111) terraces. When CO was dosed at the hydrogen adsorption potential region, CO adsorbed in both atop (CO L ) and bridge (CO B ) configurations, as well as coadsorbed water species, were detected on the Rh film electrode. A partial interconversion of spectroscopic bands due to the CO displacement from bridge to atop sites was found during the anodic potential scan, revealing that there is a potential-dependent preference of CO adsorption sites on Rh surfaces. Our data indicate that CO oxidation on Rh electrode surface in acidic media involves coadsorbed water and follows the nucleation and growth model of a Langmuir-Hinshelwood type reaction

Genetic divergence in nuclear genomes between populations of Fagus crenata along the Japan Sea and Pacific sides of Japan.

Science.gov (United States)

Hiraoka, Koichi; Tomaru, Nobuhiro

2009-05-01

Genetic diversity and structure in Fagus crenata were studied by analyzing 14 nuclear microsatellite loci in 23 populations distributed throughout the species' range. Although population differentiation was very low (F (ST) = 0.027; R (ST) = 0.041), both neighbor-joining tree and Bayesian clustering analyses provided clear evidence of genetic divergence between populations along the Japan Sea (Japan Sea lineage) and Pacific (Pacific lineage) sides of Japan, indicating that physical barriers to migration and gene flow, notably the mountain ranges separating the populations along the Japan Sea and Pacific sides, have promoted genetic divergence between these populations. The two lineages of the nuclear genome are generally consistent with those of the chloroplast genome detected in a previous study, with several discrepancies between the two genomes. Within-population genetic diversity was generally very high (average H (E) = 0.839), but decreased in a clinal fashion from southwest to northeast, largely among populations of the Japan Sea lineage. This geographical gradient may have resulted from the late-glacial and postglacial recolonization to the northeast, which led to a loss of within-population genetic diversity due to cumulative founder effects.

APWR - Mitsubishi, Japan/Westinghouse, USA

International Nuclear Information System (INIS)

Aeba, Y.; Weiss, E.H.

1999-01-01

Nuclear power generated by light water reactors accounts for approximately 1/3 of Japan's power supply. Development of the Advanced Pressurized Water Reactor (APWR) was initiated by five PWR electric power companies (Hokkaido, Kansai, Shikoku, Kyushu and Japan Atomic Power), Mitsubishi Heavy Industries, and Westinghouse, with a view to providing a nuclear power source to meet future energy demand in Japan. The APWR was developed based on the results of the Improvement and Standardization Program, promoted by the Ministry of International Trade and Industry, with reconsideration of the needs of age, such as construction cost reduction, enhanced safety and increased reliability. One of the important concepts of the APWR is its large power rating that decreases the construction cost per unit of electric generation capacity. Though the electric output was lower at the early stage of basic design than it is now, uprating to approximately 1530 MW is achieved based on the results of design progress and high efficiency improvements to the steam turbine and reactor coolant pumps. Furthermore, the APWR remarkably enhances reliability, safety operability and maintainability by introducing new technologies that include a radial reflector and advanced accumulators. The first APWR is planned to be built at Tsuruga No. 3 and No. 4 by the Japan Atomic Power Company and will be the largest commercial operation plant in the early 21st century. (author)

Behavior of irradiated ATR/MOX fuel under reactivity initiated accident conditions (Joint research)

International Nuclear Information System (INIS)

Sasajima, Hideo; Fuketa, Toyoshi; Nakamura, Takehiko; Nakamura, Jinichi; Uetsuka, Hiroshi

2000-03-01

Pulse irradiation experiments with irradiated ATR/MOX fuel rods of 20 MWd/kgHM were conducted at the NSRR in JAERI to study the transient behavior of MOX fuel rod under reactivity initiated accident conditions. Four pulse irradiation experiments were performed with peak fuel enthalpy ranging from 335 J/g to 586 J/g, resulted in no failure of fuel rods. Deformation of the fuel rods due to PCMI occurred in the experiments with peak fuel enthalpy above 500 J/g. Significant fission gas release up to 20% was measured by rod puncture measurement. The generation of fine radial cracks in pellet periphery, micro-cracks and boundary separation over the entire region of pellet were observed. These microstructure changes might contribute to the swelling of fuel pellets during the pulse irradiation. This could cause the large radial deformation of fuel rod and high fission gas release when the pulse irradiation conducted at relatively high peak fuel enthalpy. In addition, fine grain structures around the plutonium spot and cauliflower structure in cavity of the plutonium spot were observed in the outer region of the fuel pellet. (author)

Urgent Safety Measures in Japan after Great East Japan Earthquake

International Nuclear Information System (INIS)

Taniura, Wataru; Otani, Hiroyasu

2012-01-01

Due to tsunami triggered by the Great East Japan Earthquake, the operating and refueling reactor facilities at Fukushima Dai-ichi and Dai-ni Nuclear Power Plants caused a nuclear hazard. Given the fact, Japanese electric power companies voluntarily began to compile various urgent measures against tsunami. And then the Nuclear and Industrial Safety Agency (NISA) ordered the licensees to put into practice the voluntarily compiled urgent safety measures, in order to ensure the effectiveness of the means for recovering cooling functions along with avoiding the release of radioactive substances to the possible minimum, even if a huge tsunami following a severe earthquake hits nuclear power plants. The following describes the state and the effect of the urgent safety measures implemented for 44 reactors (under operation) and 1 reactor (under construction) in Japan and also describes the measures to be implemented by the licensees of reactor operation in the future.

Challenge to the enhancement of LWRs in Japan

International Nuclear Information System (INIS)

Inoue, T.

1996-01-01

Japan was way behind western countries in nuclear power development. This is partly because of the defeat in the second world war which bound Japan to the ban on nuclear power development. As a result, Japan was obliged to take the policy of introducing the power reactor, and its technology, from overseas advanced countries in order to promote the development of power reactors for its own country. (orig.)

The internationalization of Japan

International Nuclear Information System (INIS)

Kuroki, T.E.

1989-01-01

There are growing tensions and frictions between the U.S. and Japan. Among them are science and technology issues that relate to the development of superconductor technology, as well as economic, trade and agricultural issues. The structure of this friction is very complex. There are many interconnected issues that cannot be resolved one by one. This article focuses on the relationship between the U.S. and Japan. Some of the complexities behind the issues are discussed by defining different notions of internationalization and by presenting the positive and negative aspects of the Japanese approach that affects the future cooperation and competition between our nations in the area of superconductivity

Divorce in contemporary Japan.

Science.gov (United States)

Fukurai, H; Alston, J

1990-10-01

Data from the 1985-86 Japanese census are analysed to explore the determinants of the divorce rates in Japan's forty-seven prefectures, using two theoretical models: (a) the social integration model, which is shown to have a greater utility in predicting Japanese divorce levels than (b), the human capital model. Female emigration patterns play a significant role in affecting the divorce rate. Population increase and net household income are also important predictors of the Japanese divorce rate and urbanization has a great influence in modern Japan. Demographic and aggregate variables such as migration, urbanization, and socioeconomic factors are useful when organized under a social integration model.

Japan's approach to monetary policy

OpenAIRE

Giovanni P. Olivei

2002-01-01

The goal of monetary policy as conducted by the Bank of Japan is to contribute to the sound development of the national economy through the pursuit of price stability. The objective of price stability, however, is not precisely defined as it has been for other central banks. Following the implementation of the new Bank of Japan Law in 1998, the monetary policy framework is characterized by central bank independence, the primacy of the price stability objective, instrument independence, and po...

Present conditions and future tasks of Japan`s ship machinery industry; Nihon hakuyo kogyo no genjo to kongo no kadai ni tsuite

Energy Technology Data Exchange (ETDEWEB)

Akahoshi, S. [Ministry of Transportation, Tokyo (Japan)

1994-09-01

Although Japan`s shipbuilding industry has been maintaining a seat for the first place in the world over 30 years or more in a contract award quantity for the new shipbuilding work, Korea got ahead of Japan and has come to be a top at the first time in 1993. Even though the various factors could be thought, it has become a turning point to be reconsidered how Japan`s shipbuilding industry should be from now on for the naval architects and persons concerned in Japan. While Japan`s production of the maritime industrial products has been in general showing the recovering tendency by a demand restoration of the large scale vessels and so forth since 1987 afterward, on the other hand, affected by an influence of the recession of the world economic activities, the total production amount in 1993 has turned to a little bit decrease and its amount was 831.8 billion yen (a ratio to the previous year: 96.4%). Though a business environment surrounding the maritime industry, in order to successively continue the work shop site inside this country as an important industry through this industry from now on, it is thought that a restoration of the price competition should be made an effort on one hand by a standardization of the maritime equipments and tools making an exclusion of the wastefulness as an object, and a strengthening of the international competitiveness at the non-price aspects such as a functionality of the products, a reliability and so forth is indispensable on the other hand. 6 figs., 3 tabs.

Situation of nuclear industry in Japan

International Nuclear Information System (INIS)

2002-08-01

This document is a reprint of a note published by the nuclear service of the French embassy in Japan. It evokes the present day situation of nuclear facilities in Japan, the public acceptance and its attitude in front of accidents, the national energy program, the deregulation and competitiveness of nuclear power, the carrying out of the nuclear program, the future reactors, the fast neutron reactors, the dismantling activities, the fuel enrichment and reprocessing of spent fuels, the use of MOX fuel, the off-site storage, the vitrified and radiological wastes, the geological disposal of wastes, the prospects of the nuclear program, the companies involved in the Japan nuclear industry, the French-Japanese bilateral cooperation, and the ITER project in the domain of nuclear fusion. (J.S.)

ATR-FTIR and Raman spectroscopic investigation of the electroporation-mediated transdermal delivery of a nanocarrier system containing an antitumour drug.

Science.gov (United States)

Balázs, Boglárka; Sipos, Péter; Danciu, Corina; Avram, Stefana; Soica, Codruta; Dehelean, Cristina; Varju, Gábor; Erős, Gábor; Budai-Szűcs, Mária; Berkó, Szilvia; Csányi, Erzsébet

2016-01-01

The aim of the present work was the optimization of the transdermal delivery of a lyotropic liquid crystal genistein-based formulation (LLC-GEN). LLC was chosen as medium in view of the poor solubility of GEN in water. Membrane diffusion and penetration studies were carried out with a Franz diffusion cell, through a synthetic membrane in vitro, a chick chorioallantoic membrane ex ovo, and ex vivo excised human epidermis. Thereafter, LLC-GEN was combined with electroporation (EP) to enhance the transdermal drug delivery. The synergistic effect of EP was verified by in vivo ATR-FTIR and ex vivo Raman spectroscopy on hairless mouse skin.

Delphi survey of issues after the Great East Japan Earthquake

International Nuclear Information System (INIS)

Maeda, Yasunobu; Seo, Kami; Motoyoshi, Tadahiro; Okada, Shinya

2011-01-01

The Great East Japan Earthquake on March 11, 2011 has catastrophic impacts on Japan. Japan is currently on the way to recovery. However, as the damage on the country as well as society is so serious, Japanese society is urged to change some systems including hazard management, energy policy, information systems and city planning. These changes are accompanied with social group realignments, thus necessarily followed by various risks. To cope with these risk issues, SRA-Japan established the special research committee for the Great East Japan Earthquake. The aim of the committee is, from viewpoints of risk analysts, to create and relate messages about risk issues in 2-3 years, in ten years and in thirty years from the earthquake. To do this, the committee garners SRA-Japan member's opinions about possible risks in Japan by using Delphi method. In SRA-Japan, there are over 600 members in interdisciplinary fields from various backgrounds, thus the messages are expected to be helpful for Japanese society to lower its risks and to optimize the resource allocation. The research is now underway. An interim report will be presented. (author)

Fuel combustion in thermal power plants in Japan

Energy Technology Data Exchange (ETDEWEB)

Kotler, V.R.

1983-11-01

The position of black coal in the energy balance of Japan is discussed. About 75% of electric energy is produced by thermal power plants. Eighty-five per cent of electricity is produced by power plants fired with liquid fuels and 3% by coal fired plants. Coal production in Japan, the forecast coal import to the country by 1990 (132 Mt/year), proportion of coal imported from various countries, chemical and physical properties of coal from Australia, China and Japan are discussed. Coal classification used in Japan is evaluated. The following topics associated with coal combustion in fossil-fuel power plants in Japan are discussed: coal grindability, types of pulverizing systems, slagging properties of boiler fuel in Japan, systems for slag removal, main types of steam boilers and coal fired furnaces, burner arrangement and design, air pollution control from fly ash, sulfur oxides and nitrogen oxides, utilization of fly ash for cement production, methods for removal of nitrogen oxides from flue gas using ammonia and catalysts or ammonia without catalysts, efficiency of nitrogen oxide control, abatement of nitrogen oxide emission from boilers by flue gas recirculation and reducing combustion temperatures. The results of research into air pollution control carried out by the Nagasaki Technical Institute are reviewed.

Kirishima, Japan Images

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — The shield volcano consists of more than 20 eruptive centers over a 20 x 30 km area that also includes Japan's first national park. Sixty-nine eruptions have been...

78 FR 66767 - Chlorinated Isocyanurates From China and Japan

Science.gov (United States)

2013-11-06

...)] Chlorinated Isocyanurates From China and Japan Determinations On the basis of the record \\1\\ developed in the... reason of imports from China and Japan of chlorinated isocyanurates, provided for in subheadings 2933.69... (LTFV) from Japan and subsidized by the Government of China.\\2\\ \\1\\ The record is defined in sec. 207.2...

The scale of biomass production in Japan

Energy Technology Data Exchange (ETDEWEB)

Matsumura, Yukihiko [School of Engineering, Hiroshima University, 1-4-1 Kagamiyama, Higashihiroshima-shi 739-8527 (Japan); Inoue, Takashi; Fukuda, Katsura [Global Warming Research Department, Mitsubishi Research Institute, Inc., 2-3-6 Ohtemachi, Chiyoda-ku, Tokyo 100-8141 (Japan); Komoto, Keiichi; Hada, Kenichiro [Renewable energy Team, Environment, Natural Resources and Energy Division, Mizuho Information and Research Institute, Inc., 2-3 Kanda-nishikicho, Chiyoda-ku, Tokyo 101-8443 (Japan); Hirata, Satoshi [Technical Institute, Kawasaki Heavy Industries, Ltd., 1-1 Kawasakicho, Akashi-shi, Hyogo 673-8666 (Japan); Minowa, Tomoaki [Biomass Recycle Research Laboratory, National Institute of Advanced and Industrial Science and Technology, 2-2-2 Hiro, Suehiro, Kure-shi, Hiroshima 737-0197 (Japan); Yamamoto, Hiromi [Socioeconomic Research Center, Central Research Institute of Electric Power Industry, 1-6-1 Ohtemachi, Chiyoda-ku, Tokyo 100-8126 (Japan)

2005-11-01

Policymakers working to introduce and promote the use of bioenergy in Japan require detailed information on the scales of the different types of biomass resources generated. In this research, the first of its type in Japan, the investigators reviewed various statistical resources to quantify the scale distribution of forest residues, waste wood from manufacturing, waste wood from construction, cattle manure, sewage sludge, night soil, household garbage, and waste food oil. As a result, the scale of biomass generation in Japan was found to be relatively small, on the average is no more than several tons in dry weight per day. (author)

SUV2, which encodes an ATR-related cell cycle checkpoint and putative plant ATRIP, is required for aluminium-dependent root growth inhibition in Arabidopsis.

Science.gov (United States)

Sjogren, Caroline A; Larsen, Paul B

2017-09-01

A suppressor mutagenesis screen was conducted in order to identify second site mutations that could reverse the extreme hypersensitivity to aluminium (Al) seen for the Arabidopsis mutant, als3-1. From this screen, it was found that a loss-of-function mutation in the previously described SUV2 (SENSITIVE TO UV 2), which encodes a putative plant ATRIP homologue that is a component of the ATR-dependent cell checkpoint response, reversed the als3-1 phenotype. This included prevention of hallmarks associated with als3-1 including Al-dependent terminal differentiation of the root tip and transition to endoreduplication. From this analysis, SUV2 was determined to be required for halting cell cycle progression and triggering loss of the quiescent centre (QC) following exposure to Al. In conjunction with this, SUV2 was found to have a similar role as ATR, ALT2 and SOG1 in Al-dependent stoppage of root growth, all of which are required for promotion of expression of a suite of genes that likely are part of an Al-dependent DNA damage transcriptional response. This work argues that these Al response factors work together to detect Al-dependent damage and subsequently activate a DNA damage response pathway that halts the cell cycle and subsequently promotes QC differentiation and entrance into endocycling. © 2017 John Wiley & Sons Ltd.

Entomological journals and publishing in Japan.

Science.gov (United States)

Fukatsu, Takema

Here I present an overview of entomological journals and publishing in Japan, thereby providing a convenient portal to the valuable scientific resources for the world's entomological researchers and scientific communities. Currently, except for several international journals published fully in English such as Applied Entomology and Zoology and Entomological Science , many entomological and entomology-related journals in Japan are not indexed by major scientific databases like Web of Science, and therefore they are neither conveniently recognizable nor accessible for the world's entomological communities. However, I point out that many of the contents of such journals are freely available via Japan's public platforms for electronic scientific literature, Japan Science and Technology Information Aggregator, Electronic (J-stage) or Citation Information by National Institute of Informatics (CiNii). Here I list 32 entomological and entomology-related societies and their 45 journals, the majority of which belong to either the Union of Japanese Societies for Insect Sciences (UJSIS), the Union of the Japanese Societies for Systematic Biology (UJSSB), the Union of Japanese Societies for Natural History (UJSNH), or the Union of Japanese Societies for Biological Science (UJSBS), with their respective URL and open-access availability.

Japan, new agenda for energy security

International Nuclear Information System (INIS)

Chrisstoffels, J.H.

2007-07-01

The author argues that the Japanese government will actively seek to strengthen security of supply. Japan has almost no domestic energy reserves. It is Asia's leading energy importer, but it faces increasing competition for resources from China and India. This has led to growing concern among Japanese policy makers. In the spring of 2007 a new Basic Energy Law was drafted that offers a strategic change of direction, away from a policy emphasis on free (energy) trade promotion and liberalisation of domestic energy markets. Japan will boost energy diplomacy towards energy producing countries and increase financial guarantees to Japanese oil and gas development companies. At the same time, Japan will raise efforts to lower its dependency on foreign oil and gas. To this end, it has set ambitious targets for further nuclear power development and the promotion of bio-trade. The author points at a multitude of complexities that may impede the success of these policies, Still, EU policy makers should be aware that Japan's energy policy has changed, driven by the conviction that markets alone cannot be relied upon to guarantee a secure supply of energy. [nl

Japan’s Self Defense Forces After the Great East Japan Earthquake: Toward a New Status Quo

Science.gov (United States)

2012-03-01

Retrenchment, SDF Status Quo, Humanitarian Aid Disaster Relief, U.S.– Japan Security Alliance, Japanese Economy , Japanese Norms, Pacifism, Anti...Chemical, Biological, Radiological, and Nuclear CRF Central Readiness Force DDF Dynamic Defense Force DPJ Democratic Party of Japan EAC East...LNG Liquefied Natural Gas METI Ministry of Economy , Trade, and Industry MINUSTAH United Nations Stabilization Mission in Haiti MOD Ministry

Status Report on the Fabrication of Fuel Cladding Chemical Interaction Test Articles for ATR Irradiations

Energy Technology Data Exchange (ETDEWEB)

Field, Kevin G. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Howard, Richard H. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

2015-09-28

FeCrAl alloys are a promising new class of alloys for light water reactor (LWR) applications due to their superior oxidation and corrosion resistance in high temperature environments. The current R&D efforts have focused on the alloy composition and processing routes to generate nuclear grade FeCrAl alloys with optimized properties for enhanced accident tolerance while maintaining properties needed for normal operation conditions. Therefore, the composition and processing routes must be optimized to maintain the high temperature steam oxidation (typically achieved by increasing the Cr and Al content) while still exhibiting properties conducive to normal operation in a LWR (such as radiation tolerance where reducing Cr content is favorable). Within this balancing act is the addition of understanding the influence on composition and processing routes on the FeCrAl alloys for fuel-cladding chemical interactions (FCCI). Currently, limited knowledge exists on FCCI for the FeCrAl-UO₂ clad-fuel system. To overcome the knowledge gaps on the FCCI for the FeCrAl-UO2 clad-fuel system a series of fueled irradiation tests have been developed for irradiation in the Advanced Test Reactor (ATR) housed at the Idaho National Laboratory (INL). The first series of tests has already been reported. These tests used miniaturized 17x17 PWR fuel geometry rodlets of second-generation FeCrAl alloys fueled with industrial Westinghouse UO₂ fuel. These rodlets were encapsulated within a stainless steel housing.To provide high fidelity experiments and more robust testing, a new series of rodlets have been developed deemed the Accident Tolerant Fuel Experiment #1 Oak Ridge National Laboratory FCCI test (ATF-1 ORNL FCCI). The main driving factor, which is discussed in detail, was to provide a radiation environment where prototypical fuel-clad interface temperatures are met while still maintaining constant contact between industrial fuel and the candidate cladding alloys

Central bank capital, financial strength, and the Bank of Japan

OpenAIRE

Thomas F. Cargill

2006-01-01

This Economic Letter addresses central bank capital and financial strength in the context of Bank of Japan policy (Cargill 2005). Specifically, it reviews general considerations about central bank capital and financial strength, discusses recent Bank of Japan policy in the context of capital structure, evaluates the Bank of Japan's concern in the context of the broader issue of central bank independence, and draws some lessons from recent Bank of Japan policy.

Current asthma deaths among adults in Japan

Directory of Open Access Journals (Sweden)

Tsugio Nakazawa

2004-01-01

Full Text Available Recent asthma deaths were examined from yearly reports of the Ministry of Health, Labor and Welfare of Japan and from reports published by the Japan Asthma Death Investigation Committee on 811 deaths over the period 1992–2000. The rate and number of recent asthma deaths in Japan have been decreasing rapidly. Most asthma deaths were of patients aged 70–90 years and there has been a marked trend for increased asthma deaths in the elderly. As for the circumstances surrounding the deaths, sudden death, unstable sudden aggravation and intermittent aggravation were mainly noted. Respiratory infections, fatigue and stress were the major courses of fatal attacks contributing to deaths due to asthma. Many of the patients who died from asthma had been diagnosed as having as moderate to severe asthma and many had non-atopic asthma. There are some reports that suggest that the recent decrease in asthma deaths in Japan is correlated with the use of inhaled cortico- steroids.

Elementary particle theory in Japan, 1930-1960

International Nuclear Information System (INIS)

Brown, L.M.; Kawabe, Rokuo; Konuma, Michiji; Maki, Ziro

1991-01-01

The present volume consists of the combined proceedings of two Japan-USA Collaborative Workshops, organized to explore historical developments of particle theory in Japan during the period 1930-1960, i.e., the three decades that include the birth and development of Meson Theory. The first phase of workshops was held during September 1978-July 1979 and the second during July 1984-September 1985. The original versions of these proceedings were published informally; namely, the former was distributed as a series of preprints of the Yukawa Institute (then called RIFP) entitled 'Particle Physics in Japan, 1930-50 Vol. I, II' (RIFP-407 and -408, September 1980); the latter was issued in the form of camera-ready printing from Yukawa Hall Archival Library (YHAL) in May 1988, under the title 'Elementary Particle Theory in Japan, 1935-1960'. Only a small number of copies were printed for both sets of proceedings due to financial limitations of the project. (author)

Delays in clinical development of neurological drugs in Japan.

Science.gov (United States)

Ikeda, Masayuki

2017-06-28

The delays in the approval and development of neurological drugs between Japan and other countries have been a major issue for patients with neurological diseases. The objective of this study was to analyze factors contributing to the delay in the launching of neurological drugs in Japan. We analyzed data from Japan and the US for the approval of 42 neurological drugs, all of which were approved earlier in the US than in Japan, and examined the potential factors that may cause the delay of their launch. Introductions of the 42 drugs in Japan occurred at a median of 87 months after introductions in the US. The mean review time of new drug applications for the 20 drugs introduced in Japan in January 2011 or later (15 months) was significantly shorter than that for the other 22 drugs introduced in Japan in December 2010 or earlier (24 months). The lag in the Japan's review time behind the US could not explain the approval delays. In the 31 of the 42 drugs, the application data package included overseas data. The mean review time of these 31 drugs (17 months) was significantly shorter than that of the other 11 drugs without overseas data (26 months). The mean approval lag behind the US of the 31 drugs (78 months) was also significantly shorter than that of the other 11 drugs (134 months). These results show that several important reforms in the Japanese drug development and approval system (e.g., inclusion of global clinical trial data) have reduced the delays in the clinical development of neurological drugs.

Superconducting magnet development in Japan

International Nuclear Information System (INIS)

Yasukochi, K.

1983-01-01

The present state of R and D works on the superconducting magnet and its applications in Japan are presented. On electrical rotating machines, 30 MVA superconducting synchronous rotary condenser (Mitsubishi and Fuji) and 50 MVA generator are under construction. Two ways of ship propulsion by superconducting magnets are developing. A superconducting magnetically levitated and linear motor propelled train ''MAGLEV'' was developed by the Japan National Railways (JNR). The superconducting magnet development for fusion is the most active field in Japan. The Cluster Test program has been demonstrated on a 10 T Nb 3 Sn coil and the first coil of Large Coil Task in IEA collaboration has been constructed and the domestic test was completed in JAERI. These works are for the development of toroidal coils of the next generation tokamak machine. R and D works on superconducting ohmic heating coil are in progress in JAERI and ETL. The latter group has constructed 3.8 MJ pulsed coil. A high ramp rate of changing field in pulsed magnet, 200 T/s, has been tested successfully. High Energy Physics Laboratory (KEK) are conducting active works. The superconducting μ meson channel and π meson channel have been constructed and are operating successfully. KEK has also a project of big accelerator named ''TRISTAN'', which is similar to ISABELLE project of BNL. Superconducting synchrotron magnets are developed for this project. The development of superconducting three thin wall solenoid has been started. One of them, CDF, is progressing under USA-Japan collaboration

Japan's new basic energy plan

International Nuclear Information System (INIS)

Duffield, John S.; Woodall, Brian

2011-01-01

In June 2010, the Japanese cabinet adopted a new Basic Energy Plan (BEP). This was the third such plan that the government has approved since the passage of the Basic Act on Energy Policy in 2002, and it represents the most significant statement of Japanese energy policy in more than four years, since the publication of the New National Energy Strategy (NNES) in 2006. Perhaps more than its predecessors, moreover, the new plan establishes a number of ambitious targets as well as more detailed measures for achieving those targets. Among the targets are a doubling of Japan's 'energy independence ratio,' a doubling of the percentage of electricity generated by renewable sources and nuclear power, and a 30 percent reduction in energy-related CO 2 emissions, all by 2030. This paper explains the origins of the 2010 BEP and why it was adopted. It then describes the content of the plan and how it differs from the NNES. A third section analyzes the appropriateness of the new goals and targets contained in the BEP and their feasibility, finding that achievement of many of the targets was likely to be quite challenging even before the March 2011 earthquake, tsunami, and nuclear crisis. - Highlights: → Origins of Japan's new Basic Energy Plan. → Content of Japan's new Basic Energy Plan. → Feasibility of achieving the targets in Japan's new Basic Energy Plan. → Impact of 2011 earthquake and tsunami on Japanese energy policy.

A review of fast reactor programme in Japan

International Nuclear Information System (INIS)

1998-01-01

This report describes the development and activities on fast reactor in Japan for the period of April 1996 - March 1997. During this period, the 30th duty cycle operation has been started in the Experimental Fast Reactor ''''Joyo''''. The cause investigation on the sodium leak incident has completed and the safety examination are being performed in the Prototype Fast Breeder Reactor ''''Monju''''. The three years design study since FY1994 on the plant optimization of the Demonstration FBR has been completed by the Japan Atomic Power Company (JAPC). Related research and development works are underway at several organizations under the discussion and coordination of the Japanese FBR R and D Steering Committee, which is composed of Power Reactor and Nuclear Fuel Development Corporation (PNC), JAPC, Japan Atomic Energy Research Institute (JAERI) and Central Research Institute of Electric Power Industry (CRIEPI). In November 1996, the Japan Atomic Energy Commission (JAEC) established a Social Gathering Meeting to discuss generally the significance of FBR development in Japan for the future. (author)

Japan's Ambivalent Diplomacy on Climate Change

International Nuclear Information System (INIS)

Pajon, C.

2010-01-01

Japan often pictures itself as an environmental leader. While many examples of Japan's actions against climate change are in line with global climate change norms, others can be in opposition to them. This study, based on first hand interviews with Japanese policy makers and actors from the private and civil society sector, gives an informed perspective on the process through which Japan came to integrate and implement, at the domestic level, the international objective of climate change prevention, pointing out the discrepancies, tensions or synergies that emerged. As the current governance on climate change, and particularly after the Copenhagen conference, tends to reassert the importance of the national level against the global one, the Japanese case can serve as an example of tools and strategies that fully integrate the domestic level

ATR-FTIR Based Pre and Post Formulation Compatibility Studies for the Design of Niosomal Drug Delivery System Containing Nonionic Amphiphiles and Chondroprotective Drug

International Nuclear Information System (INIS)

Khan, M.I.; Madni, A.; Ahmad, S.; Rehmanand, M.; Mahmood, M.A.; Khan, A.

2015-01-01

Pharmaceutical compatibility studies are considered as the most important and first screening stage during development of pharmaceutical drug product. Attenuated total reflectance/fourier transform infrared (ATR-FTIR) is one of the techniques currently available to pharmaceutical scientists for investigating the compatibilities between active drug and inactive pharmaceutical ingredients. The present study was designed to assess the interaction among different niosomes forming components i.e nonionic amphiphiles and chondroprotective/antiinflamatory drug Diacerein by ATR-FTIR method. Physical mixtures and niosomes were prepared by physical mixing and thin film hydration method, respectively. The individual niosomal components, physical mixtures as well as niosomal formulations were analyzed. The spectra of Diacerein showed characteristic peaks at 3300 cm/sup -1/(-COOH) and 760 cm/sup -1/(msubstituted benzene), Span 60 at 2916 cm/sup -1/(-OH), Span 80 at 1740 cm/sup -1/(5- membered ring), Span 85 at 1643 cm/sup -1/(ketone with 5-membered ring), Tween 20 at 1734 cm/sup 1/ (5-membered ring) and Tween 80 at 3488 cm/sup -1/(-OH). The characteristic peaks of Diacerein were present in niosomal formulations with slight shift at 3355-3379 cm/sup -1/(-COOH) and 760-770 cm/sup -1/(m-substituted benzene). This work suggested no significant interaction in characteristic peaks of Diacerein after combining with nonionic surfactants as physical mixtures and niosomal formulations which proposed potential for niosomes to encapsulate diacerein in their micro vicinity. (author)

Getah Virus Infection among Racehorses, Japan, 2014

Science.gov (United States)

Bannai, Hiroshi; Tsujimura, Koji; Kobayashi, Minoru; Kikuchi, Takuya; Yamanaka, Takashi; Kondo, Takashi

2015-01-01

An outbreak of Getah virus infection occurred among racehorses in Japan during September and October 2014. Of 49 febrile horses tested by reverse transcription PCR, 25 were positive for Getah virus. Viruses detected in 2014 were phylogenetically different from the virus isolated in Japan in 1978. PMID:25898181

Datafile: [nuclear power in] Japan

International Nuclear Information System (INIS)

Anon.

1989-01-01

Japan is third after the USA and France in terms of the Western World's installed nuclear capacity, but it has by far the largest forward programme. Great effort is also being put into the fuel cycle and advanced reactors. There is close co-operation between the government, utilities and manufacturers, but Japan has not sought to export reactors. The government has responded to the growing public opposition to nuclear power with a massive increase in its budget for public relations. Details of the nuclear power programme are given. (author)

Overview of aerosol properties associated with air masses sampled by the ATR-42 during the EUCAARI campaign (2008

Directory of Open Access Journals (Sweden)

S. Crumeyrolle

2013-05-01

Full Text Available Within the frame of the European Aerosol Cloud Climate and Air Quality Interactions (EUCAARI project, the Météo-France aircraft ATR-42 performed 22 research flights over central Europe and the North Sea during the intensive observation period in May 2008. For the campaign, the ATR-42 was equipped to study the aerosol physical, chemical, hygroscopic and optical properties, as well as cloud microphysics. For the 22 research flights, retroplume analyses along the flight tracks were performed with FLEXPART in order to classify air masses into five sectors of origin, allowing for a qualitative evaluation of emission influence on the respective air parcel. This study shows that the extensive aerosol parameters (aerosol mass and number concentrations show vertical decreasing gradients and in some air masses maximum mass concentrations (mainly organics in an intermediate layer (1–3 km. The observed mass concentrations (in the boundary layer (BL: between 10 and 30 μg m−3; lower free troposphere (LFT: 0.8 and 14 μg m−3 are high especially in comparison with the 2015 European norms for PM2.5 (25 μg m−3 and with previous airborne studies performed over England (Morgan et al., 2009; McMeeking et al., 2012. Particle number size distributions show a larger fraction of particles in the accumulation size range in the LFT compared to BL. The chemical composition of submicron aerosol particles is dominated by organics in the BL, while ammonium sulphate dominates the submicron aerosols in the LFT, especially in the aerosol particles originated from north-eastern Europe (~ 80%, also experiencing nucleation events along the transport. As a consequence, first the particle CCN acting ability, shown by the CCN/CN ratio, and second the average values of the scattering cross sections of optically active particles (i.e. scattering coefficient divided by the optical active particle concentration are increased in the LFT compared to BL.

Japan's fuel recycling policy

International Nuclear Information System (INIS)

Anon.

1991-01-01

The Atomic Energy Commission (AEC) has formulated Japanese nuclear fuel recycling plan for the next 20 years, based on the idea that the supply and demand of plutonium should be balanced mainly through the utilization of plutonium for LWRs. The plan was approved by AEC, and is to be incorporated in the 'Long term program for development and utilization of nuclear energy' up for revision next year. The report on 'Nuclear fuel recycling in Japan' by the committee is characterized by Japanese nuclear fuel recycling plan and the supply-demand situation for plutonium, the principle of the possession of plutonium not more than the demand in conformity with nuclear nonproliferation attitude, and the establishment of a domestic fabrication system of uranium-plutonium mixed oxide fuel. The total plutonium supply up to 2010 is estimated to be about 85 t, on the other hand, the demand will be 80-90 t. The treatment of plutonium is the key to the recycling and utilization of nuclear fuel. By around 2000, the private sector will commercialize the fabrication of the MOX fuel for LWRs at the annual rate of about 100 t. Commitment to nuclear nonproliferation, future nuclear fuel recycling program in Japan, MOX fuel fabrication system in Japan and so on are reported. (K.I.)

Childbirth in aristocratic households of Heian Japan.

Science.gov (United States)

Andreeva, Anna

2014-01-01

This paper focuses on childbirth in Japan's aristocratic households during the Heian period (794-1185). Drawing on various sources, including court diaries, visual sources, literary records, and Japan's first medical collection, with its assortment of gynaecological and obstetric prescriptions, as well as Buddhist and other ritual texts, this short excursion into the cultural history of childbirth offers an insight into how childbirth was experienced and managed in Heian Japan. In particular, it addresses the variety of ideas, knowledge systems and professionals involved in framing and supporting the process of childbirth in elite households. In so doing, it casts light on the complex background of early Japanese medicine and healthcare for women.

Introductory Overview of Stone Heritages in Japan

Science.gov (United States)

Kato, Hirokazu; Oikawa, Teruki; Fujita, Masayo; Yokoyama, Shunji

2013-04-01

As one contribution to 'Global Heritage Stone Resources' (GHSR), some stone heritages in Japan, which are nominated in the interim list, are briefly introduced. The geology of Japanese Islands where are the one of the most active areas in the history of the Earth, is very complicated. Therefore Japanese Islands consist of various kinds of minerals and rocks. Some of them were used to make stone implements and accessories. Japanese people also used to the best possible advantage to built tombstone, gate, pavement ,and the basement and wall of the large building such as temples, shrines, castles and modern buildings. 1. Stone Heritages of Pre-historical age: In the late Pleistocene and the early Holocene, ancient Japanese used obsidian cooled rapidly from rhyolitic magma.to make small implements and accessories. For example, Shirataki, Hokkaido (north island) is the largest place producing obsidian in Japan where Paleolithic people made arrowhead, knives and so on. Another example, Jade yielded in Itoigawa City, Japan Sea coast of central Japan, was made in the metamorphic rock about five hundred million years ago. Itoigawa area is only one place where jade is abundantly produced in Japan. Ancient people had been already collected and processed to ornaments although it is very hard and traded in wide area more than several thousand years ago. 2. Stone Heritages of Historical age: 2.1 Archaeological remains: In the Kofun (old mound) period (250 to 538 AD), stone burial chambers were used for old mounds to preserve against the putrefaction and to protect from the theft. For example, Ishibutai Kofun ("ishi" means "stone" and "butai" means "stage") in Nara old capital city, southwest Japan, is the largest known megalithic structure made of granite in Japan. 2.2 Stone walls of some typical castles Stones used is because of not only the rich reserves of rocks but also restriction of transportation. Osaka (second biggest city) castle, are composed of Cretaceous granite

Explaining Meiji Japans Top Down Revolution

Science.gov (United States)

2017-12-01

Operations and Reports, 1215 Jefferson Davis Highway, Suite 1204, Arlington, VA 22202-4302, and to the Office of Management and Budget, Paperwork Reduction...Modernization in Japanese Culture, ed. Donald H. Shively (Princeton, NJ: Princeton University Press, 1971), 93–97. 24 David J. Lu, Japan: A Documentary ...but also took advantage of international developments. Japan managed to alter the unequal treaties with his oversight. The Japanese began to study an

Japan's Fiscal Policy and Fiscal Reconstruction

OpenAIRE

Ihori, Toshihiro; Nakamoto, Atsushi

2005-01-01

This paper investigates the macroeconomic effects of fiscal policy and the fiscal reconstruction movement in Japan. We first summarize Japan's fiscal policy in recent years and discuss advantages and disadvantages of government deficits. Next, we investigate the macroeconomic effects of Japanese fiscal policy and evaluate the plausibility of non-Keynesian effects. We also analyze the possibility of the crowding-in effect of fiscal policy and investigate the spillover effects of deregulation. ...

Trauma and posttraumatic stress disorder in Japan: Results from the World Mental Health Japan Survey

Science.gov (United States)

Kawakami, Norito; Tsuchiya, Masao; Umeda, Maki; Koenen, Karestan C.; Kessler, Ronald C.

2014-01-01

The purpose of the study was to report the prevalence of trauma exposure and PTSD, conditional risk of PTSD associated with each trauma exposure in the community population in Japan. An interview survey was conducted of a random sample of adult residents in 11 communities of Japan. Among 4134 respondents (response rate, 55%), data from those who completed the part 2 interview (n = 1682) were analyzed with a weight for this subsample. Lifetime experiences of 27 trauma events and PTSD were assessed using the WHO-Composite International Diagnostic Interview version 3.0. Sixty percent of the part 2 sample reported exposure to at least one lifetime traumatic event. Lifetime and 12-month PTSD prevalences were 1.3% and 0.7%, respectively. Percentage of all months lived with PTSD in the population was predominantly accounted for by physical/sexual assaults and having a child with serious illness, and unexpected death of loved one. Ten percent of respondents reported “private events”, for which respondents did not have to describe the content, which accounted for 19% of months with PTSD. The lower prevalence of PTSD in Japan seems attributable to lower conditional risks of PTSD following these events, as well as different distributions of the events. The greater impact of events that occurred to loved ones rather than to oneself and “private events” on PTSD in Japan warrants further research of cross-cultural assessment of trauma exposure and cultural heterogeneity in the trauma-PTSD relationship. PMID:24572682

Atomic energy, fundamental project, 1990, Japan

International Nuclear Information System (INIS)

1990-01-01

The project is composed of the following items: 1. Synthetic strengthening countermeasure for guanrantee of safety. (1) Completion of administration for regulation, (2) promotion of research, (3) disaster prevention. 2. Promotion of nuclear power generation. 3. Establishment of nuclear fuel cycle. (1) Secure the uran-resources, (2) domestic uran-concentration, (3) reprocessing of spent fuel and utilization of recovered uran, (4) radioactive waste disposal. 4. Development of new type power reactors and utilization of plutonium (1) FBR, (2) ATR, (3) plutonium fuel processing technology. 5. Promotion of leading projects. (1) Research of nuclear fusion, (2) radiation utilization, (3) R and D of nuclear ship, (4) high temperature technology testing research. 6. Promotion of fundamental technology development, etc. 7. Subjective and active international contribution. 8. Understanding and cooperation of the nation. (M.T.)

The cause of larger local magnitude (Mj) in western Japan

Science.gov (United States)

Kawamoto, H.; Furumura, T.

2017-12-01

The local magnitude of the Japan Meteorological Agency (JMA) scale (Mj) in Japan sometimes show a significant discrepancy between Mw. The Mj is calculated using the amplitude of the horizontal component of ground displacement recorded by seismometers with the natural period of T0=5 s using Katsumata et al. (2004). A typical example of such a discrepancy in estimating Mj was an overestimation of the 2000 Western Tottori earthquake (Mj=7.3, Mw=6.7; hereafter referred to as event T). In this study, we examined the discrepancy between Mj and Mw for recent large earthquakes occurring in Japan.We found that the most earthquakes with larger Mj (>Mw) occur in western Japan while the earthquakes in northern Japan show reasonable Mj (=Mw). To understand the cause of such larger Mj for western Japan earthquakes we examined the strong motion record from the K-NET and KiK-net network for the event T and other earthquakes for reference. The observed ground displacement record from the event T shows a distinctive Love wave packet in tangential motion with a dominant period of about T=5 s which propagates long distances without showing strong dispersions. On the other hand, the ground motions from the earthquakes in northeastern Japan do not have such surface wave packet, and attenuation of ground motion is significant. Therefore, the overestimation of the Mj for earthquakes in western Japan may be attributed to efficient generation and propagation properties of Love wave probably relating to the crustal structure of western Japan. To explain this, we then conducted a numerical simulation of seismic wave propagation using 3D sedimentary layer model (JIVSM; Koketsu et al., 2012) and the source model of the event T. The result demonstrated the efficient generation of Love wave from the shallow strike-slip source which propagates long distances in western Japan without significant dispersions. On the other hand, the generation of surface wave was not so efficient when using a

Enantioselective skin permeation of ibuprofen enantiomers: mechanistic insights from ATR-FTIR and CLSM studies based on synthetic enantiomers as naphthalimide fluorescent probes.

Science.gov (United States)

Che, Qi-en; Quan, Peng; Mu, Mao; Zhang, Xinfu; Zhao, Hanqing; Zhang, Yu; You, Song; Xiao, Yi; Fang, Liang

2014-10-01

The aim of this study was to investigate the mechanisms of different skin permeability of ibuprofen racemate and enantiomers. The percutaneous permeation of ibuprofen racemate and enantiomers through rabbit normal skin and damaged skin (without stratum corneum [SC]) was investigated in vitro using side-by-side diffusion cells. With the melting temperature-membrane transport model, the flux ratio of enantiomer/racemate was calculated from their thermodynamic properties obtained by differential scanning calorimetry. Attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR) study was performed to evaluate the interaction between the enantiomers and the SC. New fluorescent probes were designed and utilized in confocal laser scanning microscopy (CLSM) study for visualization of the enantioselective permeation of the enantiomers through the intact rabbit skin. The flux of (S)-ibuprofen through normal skin was significantly higher than that of (RS)-ibuprofen and (R)-ibuprofen (p skin, there was no significant difference (p > 0.05). The predicted flux ratio of (S)-ibuprofen/(RS)-ibuprofen (2.50) was in close agreement with the experimentally determined ratio (2.48). These results were supported by ATR-FTIR and CLSM studies that indicated that a chiral environment of the skin led to the enantioselective permeation of enantiomers. The chiral nature of the SC and the different physicochemical properties of the enantiomers should be taken into account in the assessment of different skin permeability of the racemate and enantiomers. The synthetic fluorescent probes used in this study could visualize the enantioselective permeation of the chiral compounds across the skin.

75 FR 67105 - Granular Polytetrafluoroethylene Resin From Italy and Japan

Science.gov (United States)

2010-11-01

... Polytetrafluoroethylene Resin From Italy and Japan AGENCY: United States International Trade Commission. ACTION... resin from Italy and Japan. SUMMARY: The Commission hereby gives notice that it has instituted reviews... revocation of the antidumping duty orders on granular polytetrafluoroethylene resin from Italy and Japan...

Memento. Maritime transport of MOX fuels from Europe to Japan

International Nuclear Information System (INIS)

1999-07-01

The maritime transport of MOX fuels from Europe to Japan represents the last of the 3 steps of transport of the nuclear fuel reprocessing-recycling program settled between ORC (Japan), BNFL (UK) and Cogema (France). This document summarizes the different aspects of this program: the companies concerned, the physical protection measures, the US-Japan agreements (accompanying warship), the in-depth safety, the handling of MOX fuels (containers and ships), and the Japan MOX fuel needs. (J.S.)

Present status of marine environmental radioactivity survey in the sea of Japan

International Nuclear Information System (INIS)

Matsuoka, H.

1994-01-01

Science and Technology Agency has been conducting some Marine Environmental Radioactivity Surveys around Japan in cooperation with the relevant organizations (Maritime Safety Agency, Japan Meteorological Agency, Fishery Agency, National Institute of Radiological Sciences, Japan Marine Science and Technology Center, Japan Chemical Analysis Center and Marine Ecology Research Institute). Several artificial radionuclides have been detected but the main origin is supposed to be fall-out. The level trend of marine environmental radioactivity has no anomalies excepting the effect of Chernobyl Accident. The data summarized here are as follows. 1. Marine Environmental Survey of Fisheries near the Nuclear Power Stations, 2. Past Data of Marine Environmental Radioactivity around Japan (Apr. 1982 - Mar. 1991), 3. Marine Environmental Survey of the Sea of Japan (spring, 1993), 4. Marine Environmental Survey of the Sea of Japan (autumn, 1993). In addition, JAPAN-KOREA-RUSSIA JOINT EXPEDITION in the Sea of Japan will start in the middle of March. We are expecting to get valuable data through the EXPEDITION. (J.P.N.)

Measurement of conjugated linoleic acid (CLA) in CLA-rich soy oil by attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR).

Science.gov (United States)

Kadamne, Jeta V; Jain, Vishal P; Saleh, Mohammed; Proctor, Andrew

2009-11-25

Conjugated linoleic acid (CLA) isomers in oils are currently measured as fatty acid methyl esters by a gas chromatography-flame ionization detector (GC-FID) technique, which requires approximately 2 h to complete the analysis. Hence, we aim to develop a method to rapidly determine CLA isomers in CLA-rich soy oil. Soy oil with 0.38-25.11% total CLA was obtained by photo-isomerization of 96 soy oil samples for 24 h. A sample was withdrawn at 30 min intervals with repeated processing using a second batch of oil. Six replicates of GC-FID fatty acid analysis were conducted for each oil sample. The oil samples were scanned using attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR), and the spectrum was collected. Calibration models were developed using partial least-squares (PLS-1) regression using Unscrambler software. Models were validated using a full cross-validation technique and tested using samples that were not included in the calibration sample set. Measured and predicted total CLA, trans,trans CLA isomers, total mono trans CLA isomers, trans-10,cis-12 CLA, trans-9,cis-11 CLA and cis-10,trans-12 CLA, and cis-9,trans-11 CLA had cross-validated coefficients of determinations (R2v) of 0.97, 0.98, 0.97, 0.98, 0.97, and 0.99 and corresponding root-mean-square error of validation (RMSEV) of 1.14, 0.69, 0.27, 0.07, 0.14, and 0.07% CLA, respectively. The ATR-FTIR technique is a rapid and less expensive method for determining CLA isomers in linoleic acid photo-isomerized soy oil than GC-FID.

Técnicas FT-IR (PAS, UATR e Objetiva ATR Aplicadas à Caracterização de EPDM Modificada com Plasma

Directory of Open Access Journals (Sweden)

Renata Patrícia dos Santos

2014-06-01

Full Text Available Técnicas FT-IR (UATR, PAS e MIC, com objetiva ATR foram escolhidas para a caracterização da superfície da borracha de EPDM vulcanizada, após tratamento em plasma de Ar/O2 e N2/H2/Ar gerado em micro-ondas. Após tratamento, grupos oxigenados foram detectados por UATR e MIC/FT-IR, com objetiva ATR para as duas misturas gasosas, e possíveis grupos nitrogenados foram inseridos na superfície das amostras tratadas com plasma de N2/H2/Ar. A análise MIC/FT-IR sugeriu a formação de grupos em uma camada mais externa, para as amostras tratadas com plasma de N2/H2/Ar, sendo possível observar a redução na intensidade das bandas da absorção do CH2 e CH3, o que pode estar relacionado ao fato de o nitrogênio ser um gás menos permeável que o oxigênio. Diferentes velocidades, 0,05 cm.s-1 e 0,2 cm.s-1, foram utilizadas na análise PAS para avaliar a superfície após tratamento, e apenas para a velocidade 0,05 cm.s-1 com plasma de Ar/O2 alterações espectroscópicas foram detectadas. A redução na medida de ângulo de contato e o aumento na resistência ao descascamento da colagem indicaram melhoras nas propriedades adesivas da superfície. Falhas de adesão foram observadas entre a interface do filme de adesivo de PU e da borracha de EPDM, e confirmados por UATR/FT-IR.

Courtesy and the challenges of implementing smoke-free policies in Japan.

Science.gov (United States)

Bialous, Stella Aguinaga; Mochizuki-Kobayashi, Yumiko; Stillman, Frances

2006-04-01

For decades, the tobacco companies have developed a worldwide campaign to oppose the creation of smoke-free environments. Public health efforts to promote clean indoor air have been uneven throughout the world, and in few places have such efforts faced as many challenges as in Japan. The Japanese market is dominated by Japan Tobacco, which is partly owned by the government, and Philip Morris International is also present in Japan. Japan Tobacco and Philip Morris International have developed campaigns promoting courtesy and tolerance that, until recently, seem to have resonated well with the public. The companies also have supported research promoting ventilation and have funded consultants to act as experts in the area of second-hand smoke exposure. Japan is a critical country to study, partly because of the strength of Japan Tobacco in the country and the growth of Japan Tobacco International in Southeast Asia and the rest of the world, and partly because of Japan's ratification of the World Health Organization Framework Convention on Tobacco Control. This paper uses tobacco industry documents to provide an overview of the tobacco industry's scientific and political efforts to stifle the development of clean indoor measures in Japan. Learning past industry strategies may assist policymakers and advocates in the development of future public health activities.

El diálogo creativo en El cuarto de atrás : La búsqueda de una identidad en la memoria colectiva

OpenAIRE

Muñoz Åhlén, Anna-Maria

2017-01-01

Esta tesina tiene como objetivo analizar la relación entre C. y el interlocutor misterioso en El cuarto de atrás de Carmen Martín Gaite. Intentaremos averiguar qué recuerdos aparecen por medio del diálogo entre los interlocutores. Partiendo de allí, el trabajo enfocará qué recuerdos de infancia aparecen por medio del diálogo y de qué manera han influido en el personaje C. en el tiempo real de la creación de la novela. Como apoyo de interpretar la identidad del personaje nos apoyaremos princip...

Role of Bi promotion and solvent in platinum-catalyzed alcohol oxidation probed by in situ X-ray absorption and ATR-IR spectroscopy

DEFF Research Database (Denmark)

Mondelli, C.; Grunwaldt, Jan-Dierk; Ferri, D.

2010-01-01

the catalysts under working conditions using in situ X-ray absorption spectroscopy (XAS) and attenuated total reflection infrared spectroscopy (ATR-IR), aiming at uncovering the roles of the metal promoter and the reaction medium. XAS confirms that Bi is oxidized more easily than Pt, maintaining the catalytic...... surfaces than on step or kink sites. Side products, CO and benzoate species, appearing during the reaction reveal that the geometric suppression of undesired reactions does not occur to the same extent on Pt-based catalysts as on Pd, suggesting that decarbonylation of the produced aldehyde on Pt may occur...

Strontium isotopic ratios of Tertiary volcanic rocks of northeastern Honshu, Japan: implication for the spreading of the Japan Sea

International Nuclear Information System (INIS)

Kurasawa, Hajime; Konda, Tadashi.

1986-01-01

Strontium isotopic ratios of sixty-seven Tertiary volcanic rocks from the northeastern Honshu, Japan, were determined for the purpose of examining the genesis among the volcanic rocks. Two distince suites of volcanic rocks occur in the northeastern Honshu; the rocks older than 16 Ma (Monzen-Daijima Stege) of predominantly intermediate composition and the rocks younger than 16 Ma (Nishikurosawa-Funakawa Stege) with bimodal suite of mafic and felsic composition. Initial values of 87 Sr/ 86 Sr in the Teriary volcanic rocks from the northeastern Honshu, lie in the range from 0.7033 to 0.7068. High ( 87 Sr/ 86 Sr) I ratios are observed for the rocks older than 16 Ma from the Japan Sea side (H zone). It is noteworthy that the rocks younger than 16 Ma show significantly lower ( 87 Sr/ 86 Sr) I ratios in the Dewa Hill, Japan Sea coast and North Akita areas in the northeastern Honshu (L zone). The rocks younger than 16 Ma from the L zone can also be interpreted as having been originated as a mantle-diapir associated with the spreading of the Japan Sea basin. If the basaltic magma was formed from the diapir, the 87 Sr/ 86 Sr ratio would be close to the range from 0.7033 to 0.7037 as the low-Sr isotopic ratio zone (L zone) in the northeastern Honshu, Japan. (author)

Revitalizing US electronics lessons from Japan

CERN Document Server

Sprague, John

1993-01-01

Written by one of the few executives who have extensive experience in the electronics industry in both the US and Japan, this book compares market research, design, and manufacturing techniques as used in both countries. Unlike other books on theJapanese business culture, Sprague gives specific advice and recommendations about what companies can do now to compete with Japan, clearly pointing out what is and what is not adaptable from the Japanese approach to business.

Testing the Nuclear Will of Japan

Science.gov (United States)

2007-12-01

particularly the United States. This was significant because a soured economic relationship would undoubtedly affect the U.S.-Japan security...around the world, has sometimes soured its image as a serious international player.136 This is because many of the world’s great powers have extended...for International Exchange, 2005. Beer , Lawrence W. “Japan Turning the Corner.” Asian Survey 11, no. 1 (January 1971): 74 – 85. Bueno de Mesquita

Explaining Meiji Japan's top-down revolution

OpenAIRE

McClain, Kendra M.

2017-01-01

Approved for public release; distribution is unlimited Meiji Japanese leaders consisted of an oligarchy that strived to overcome Western imperialist pressures in Asia. They did so by overturning some deeply rooted Tokugawa-Era traditions in Japanese society and replaced them with Western ones. They understood that Western norms dictated world affairs, so they sought to make Japan strong along Western norms. Modeling the West provided enough traction for Japan to meet Western threats and ma...

Status report of shielding investigation in Japan

International Nuclear Information System (INIS)

Shindo, M.

1964-01-01

The Japan Atomic Energy Research Institute (JAERI) was established in 1954, and immediately proceeded with the construction of a research reactor. The first symposium in Japan on nuclear energy was held in 1957. Most of the papers presented in the field of reactor shielding were limited to shielding materials and their fabrication. In the first stage of our investigations, our efforts were devoted to practical design studies of reactor shielding. As a result of these studies, it was found that the formulae at hand for calculations were inadequate, but at that time no electronic computer was available in Japan nor were theoretical calculations very actively undertaken. Problems on nuclear ship shielding had been investigated at the Ship Research Institute, since 1956 and many fruitful results had been obtained. About that time the Japan Atomic Industry Forum started activities and took the initiative in organizing shielding research. Research workers in the shipbuilding industry in particular have been seriously studying shielding problems. Few years after the first symposium, problems concerning more fundamental studies were treated by many research workers. Shielding experiments using radioisotopes were carried out and many fruitful results were obtained. They are described in the this paper. Medium size electronic computers became available in Japan, permitting a theoretical study group to make an active contribution. They produced some codes, and their results are also described in the following sections. This constituted the second stage of our investigations. A swimming-pool reactor, JRR-4 (Japan Research Reactor-4), has been under construction at JAERI since 1962 and will become critical in autumn 1964. After characteristic tests it will be a very powerful tool for the shielding investigations. This id the beginning of the third stage of investigations

Toward freedom from cancer pain in Japan.

Science.gov (United States)

Otsuka, Kuniko; Yasuhara, Hajime

2007-01-01

Life expectancy in Japan is highest in the world. Cancer is the leading cause of mortality in Japan, accounting for about 30 percent of all deaths. Many Japanese cancer patients experience severe pain although they and their families hope to be pain free at the end of their lives. Toward that end, the consumption of morphine in Japan has increased markedly since 1989. The amount of morphine hydrochloride and morphine sulfate consumed in 2001 was 6.1 times that used in Japan in 1989. However, the amount of morphine consumed in Japan is still less than in other developed nations, and was only one-sixth of the amount used in Australia in 2001. As a result, many Japanese cancer patients experience potentially manageable cancer pain, largely because the amount of the drug used by doctors is insufficient for pain control. An increasing number of Japanese doctors now understand that their patients' quality of life is most important in end-of-life care and how to use the three step analgesic ladder of the World Health Organization (WHO). However, other doctors do not understand these issues sufficiently causing some patients to die without good pain control. Both the general population and some medical professionals misunderstand and have prejudice against the use of morphine. Patients often do not participate in decision making about medical treatment because of remaining paternalism in the relationship between Japanese doctors and patients. Thus, cancer pain management in Japan is not as effective as it can be and not all Japanese cancer patients receive appropriate management for their cancer pain. To improve outcomes for Japanese patients, it is necessary for health professional and social work students and practicing professionals to receive contemporary education including an introduction to palliative care and ethics.

Reactor construction programme in Japan

Energy Technology Data Exchange (ETDEWEB)

NONE

1959-04-15

In September last year, the Japanese Government requested the International Atomic Energy Agency to supply three tons of natural uranium for a research reactor, and the Agency has now arranged for its sale to Japan. The metal will be supplied in ingot form and after fabrication it will be used as fuel in a reactor of the natural uranium, heavy water type. The uranium will be obtained from Canada and sold to Japan by IAEA. The Agency had invited tenders for its supply, and after considering the tenders received, the Agency's Board of Governors decided that the Canadian offer to the Agency of three tons of natural uranium free of charge should be accepted and that the selling price to Japan should be US $35. 50 per kilogramme. The price takes into account Article XIV/E of the Agency's Statute which says that the Agency shall establish a scale of charges (including those for storage and handling) for materials furnished to Member States, and that the scale shall be designed to produce revenues to meet expenses in connexion with materials acquired by the Agency and costs of materials and services provided by it under agreements with one or more members. This is the first operation of its kind to be undertaken by the Agency, and the reactor for which the supply is being made will be the first in Japan to be constructed by Japanese scientists and technicians. IAEA's Board of Governors has given the necessary approval to the reactor project for which the Agency is providing assistance

[Gambling disorder in Japan].

Science.gov (United States)

Tanabe, Hitoshi

2015-09-01

Gambling disorder is a psychiatric disorder characterized by persistent and recurrent problematic gambling behavior, associated with impaired functioning, reduced quality of life, and frequent divorce and bankruptcy. Gambling disorder is reclassified in the category Substance-Related and Addictive Disorders in the DSM-5 because its clinical features closely resemble those of substance use disorders, and gambling activates the reward system in brain in much the same way drugs do. Prevalence of gambling disorder in Japan is high rate because of slot machines and pachinko game are very popular in Japan. The author recommend group psychotherapy and self-help group (Gamblers Anonymous), because group dynamics make them accept their wrongdoings related to gambling and believe that they can enjoy their lives without gambling.

China's air pollution and Japan's response to it

International Nuclear Information System (INIS)

Matsuura, S.

1995-01-01

Long-range transport of air pollutants has been a serious global environment problem. Japan's Central Research Institute of Electric Power Industry (CRIEPI) researchers are investigating the possibility of acid rain from China. So far, the acid rain that has appeared in Japan has not had a significant effect. However, because of the high economic growth projected for the coming years in China, acidic fallout from that country is likely to cause serious damage there and in Japan as well. China is one of the largest coal users in the world. One of the biggest factors making air quality worse is the lack of appropriate technologies. Although Japanese desulfurization technologies are well advanced, they cannot be directly applied to stationary pollution sources in China, because of their cost. Japan has sophisticated technologies, but lacks experience in and knowledge of alternative, intermediate, and low-technology solutions. Given this, Japan may attempt to buy pollution control technology equipment from the United States, which provides a wider range of desulfurization technologies, as a part of the Official Development Assistance program. At the same time, international support that includes the establishment of additional global environmental monitoring stations in China are also necessary

Policy of Japan of radioactive waste management

International Nuclear Information System (INIS)

Oyama, A.

1989-01-01

Development and utilization of nuclear power has been steadily pursued in Japan in order to secure a stable source of energy over a long-term period. According to the author, nuclear power plants are operated carefully and safely and have been generating electricity for more than twenty years. In fact, it now accounts for approximately 30% of total electricity generated, and has become an integral part of the energy supply in Japan. The benefits of nuclear technology are now enjoyed not only in the electricity production, but also in the fields such as medicine and other industries. Under such circumstances, radioactive waste management generated in nuclear power plants and reprocessing plants as well as in industrial and medical uses of radiation, is an important part of the nuclear energy policy, and there is also a strong public interest in this field. The government is active in formulating waste management policies based on the basic policies indicated by Japan ATomic Energy Commission (JAEC). This presentation will touch upon the future perspective of radioactive waste management policy in Japan