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Sample records for isoformes neuronales des

  1. Classification neuronale des textures de milieux terrestres naturels en télédétection radar

    Directory of Open Access Journals (Sweden)

    Ph. Mainguenaud

    2000-05-01

    Full Text Available L’image radar présente une granularité (speckle qui rend impossible l’utilisation des algorithmes classiques du traitement d’images pour la classification. Nous avons cherché à définir la notion de texture. Certaines méthodes de filtrage peuvent modifier la distribution spatiale des pixels et créer "artificiellement" une information d’évolution de la texture pour aider à la segmentation des différentes classes.

  2. Soziales Funktionsniveau und dessen neuronale Korrelate bei gesunden Menschen und bei Menschen mit Schizophrenie

    OpenAIRE

    2015-01-01

    Defizite des sozialen Funktionsniveaus sind ein Hauptmerkmal schizophrener Erkrankungen. Diese Defizite sind gut beschrieben, aber ihre neurobiologischen Grundlagen sind bislang kaum verstanden. Übergeordnetes Ziel dieser Arbeit ist die Erweiterung des Wissens über neuronale Korrelate des sozialen Funktionsniveaus sowie ein besseres Verständnis sozialer Funktionsdefizite. Ein weiteres Ziel ist die Validierung und Etablierung einer deutschen Übersetzung der Social Functioning Scale (SFS), eine...

  3. Modélisation du changement d’échelles en télédétection par une méthode neuronale : application a l’étude de l’évolution de l’occupation hivernale des sols en Bretagne

    Directory of Open Access Journals (Sweden)

    Thomas Houet

    2004-05-01

    Full Text Available Le suivi de la couverture hivernale des sols constitue un des principaux enjeux de la réduction de la pollution de la ressource en eau en Bretagne, la présence de la végétation entravant les transferts de flux polluants vers les cours d’eau. Le suivi du ratio « sols nus / sols couverts » peut être réalisé à l’aide d’instruments satellitaires de façon quotidienne à une échelle régionale, mais à une résolution spatiale grossière par des capteurs de type SPOT VEGETATION (1 km. Il peut également être effectué à une résolution spatiale fine par des capteurs tel que SPOT HRVIR (20 m mais avec une acquisition d’images une ou deux fois par hiver seulement sur un territoire plus limité. Toute évolution de ce ratio, détectée à l’échelle régionale, peut être issue de variations de la couverture hivernale des sols, mais également de l’influence de facteurs climatiques. Les changements observés doivent alors être validés par une analyse à l’échelle locale. Le lien entre les images des deux capteurs est établi à partir du développement d’une méthode neuronale basée sur une carte de Kohonen. L’originalité de cette méthode réside dans l’utilisation de la dimension temporelle pour résoudre ce problème de changement d’échelles.

  4. Die Wirkung von Tolperison auf muskuläre, kardiale und neuronale Natriumkanäle

    OpenAIRE

    Rohde, Doris

    2011-01-01

    Die Wirkung von Tolperison, einem oral applizierten Natriumkanalblocker zur Behandlung schmerzhafter Muskelverspannungen, auf muskuläre, kardiale und neuronale Natriumkanäle Hintergrund und Ziele Tolperison (Mydocalm®) wird als „Lokalanästhesie zum Schlucken“ vermarktet und ist zugelassen zur Behandlung von schmerzhaften Muskelverspannungen bei degenerativen Veränderungen des Bewegungsapparates sowie zur Behandlung von Spastizität bei neurologischen Erkrankungen. Tolperison weist strukturelle...

  5. neuronales

    Directory of Open Access Journals (Sweden)

    Israel Espinosa Martínez

    2005-01-01

    Full Text Available En los sistemas eléctricos de potencia, existen diferentes fenómenos que distorsionan la forma de onda del voltaje que se utiliza en aplicaciones críticas, estas distorsiones han cobrado muchas vidas por no saber controlarlas, reducirlas o eliminarlas de forma segura. Es necesario suministrar energía de forma eficiente y segura, no sólo a procesos automatizados y de precisión sino también a aplicaciones de calidad y médicas como una sala quirúrgica o en algún instrumento cuya precisión en sus reportes de lectura dictaminan si una persona sigue con vida o necesita someterse a una operación.

  6. Las bases celulares de las oscilaciones neuronales

    OpenAIRE

    Mario Treviño; Rafael Gutiérrez

    2007-01-01

    Una de las características de los circuitos neuronales es que sus componentes, las neuronas, pueden presentar actividad eléctrica sincrónica y, gracias a ésta, generar actividad oscilatoria. Esta actividad se ha asociado a diversas funciones fisiológicas como el procesamiento de información sensorial, la memoria, el ciclo vigiliasueño y la conciencia. La actividad oscilatoria de un circuito neuronal está mediada por i) las propiedades intrínsecas de sus células, ii) la arquitectura de sus con...

  7. Control adaptable utilizando Redes Neuronales Artificiales Polinomiales

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    E. Gómez

    2000-01-01

    Full Text Available Existen en la literatura de Control Adaptable, diferentes procedimientos en los que es posible identificar un sistema lineal. El problema fundamental es que una cantidad importante de fenómenos de la vida real son de tipo no lineal y no es tan sencillo el modelar este tipo de dinámicas. En este trabajo se presenta una forma de identificar sistemas no lineales utilizando las propiedades de las Redes Neuronales Artificiales y las técnicas de Algoritmo Genético en la optimización de su arquitectura. Adicionalmente se presenta una técnica novedosa de control adaptable para cancelar la dinámica no lineal del sistema y colocar los polos en el punto de operación deseado. Se presenta el comportamiento del algoritmo para el caso lineal yno lineal y finalmente se analiza la importancia teórica y operacional de estas técnicas.

  8. Hypnose, sommeil, placebo ? : Les réponses de la Théorie neuronale de la Cognition – Tome 2

    OpenAIRE

    Touzet, Claude

    2014-01-01

    International audience; Initialement uniquement dédié aux quelques « faits » comme :l'hypnose, le plaisir, le sommeil et les rêves, la responsabilité, l'effet placebo et la schizophréniequi n'avaient pas reçu d'explications de la part de la Théorie neuronale de la Cognition (tome 1 – Conscience, intelligence, libre-arbitre), ce second opus a rapidement dévié de sa mission originale pour intégrer des aspects encore plus controversés, comme :- le paranormal,- l'influence de l'intention sur la r...

  9. Rôles respectifs des isoformes de ferrédoxine-NADP-oxydoréductase dans la cyanobactérie Synechocystis sp. PCC 6803

    OpenAIRE

    Korn, Anja

    2010-01-01

    In photosynthetic organisms, ferredoxin:NADP oxidoreductase (FNR) provides NADPH for CO2 assimilation, but it also utilizes NADPH to provide reduced ferredoxin (Fd). The cyanobacterium Synechocystis sp. strain PCC6803 contains two FNR isoforms, a small (FNRS, 34 kDa) and a large one (FNRL, 46 kDa) that is associated with the phycobilisome (PBS), a light-harvesting complex. We purified a PBS subcomplex comprising FNRL (FNRL-PC) and compared the enzymatic properties of FNRL-PC to FNRS. FNRL-PC ...

  10. Reconocimiento automático del habla con redes neuronales artificiales

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    Diego H. Milone

    2005-01-01

    Full Text Available En la última década se han investigado intensamente los fundamentos y aplicaciones de las redes neuronales artificiales y se ha invertido un gran esfuerzo en el área del reconocimiento automático del habla. Este trabajo presenta una extensa revisión y un análisis crítico de la aplicación de redes neuronales artificiales al reconocimiento automático del habla. Se mencionan los antecedentes de la década de los 80 y se describen los trabajos que constituyen una aplicación directa de técnicas clásicas de redes neuronales artificiales. Se discuten aquellos en los que se han desarrollado nuevas arquitecturas neuronales, orientadas a resolver el problema del reconocimiento automático del habla, y se presentan también numerosos trabajos que consisten en la combinación de redes neuronales artificiales y modelos ocultos de Markov. Finalmente se realiza un análisis comparativo de los diferentes enfoques y los puntos clave que guían las investigaciones en torno a nuevos paradigmas sobre este problema. ´

  11. Un nuevo enfoque para la resolución de problemas: redes neuronales

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    Jonatán Gómez Perdomo

    2015-05-01

    Full Text Available RESUMEN El siguiente artículo  expone los lineamientos generales de un nuevo enfoque para la resolución de problemas mediante computador. Inspiradas en las redes neuronales biológicas, las redes neuronales artifíciales han  mostrado mayor efectividad para resolver problemas que los enfoques tradicionales no han  solucionado o lo han hecho de manera parcial. En la actualidad este enfoque ha ganado un gran auge, lo cual ha generado en el país grupos de investigación que están trabajando sobre sí mismo. Como parte de su trabajo  en el grupo de la Universidad Nacional, los autores desarrollan una herramienta para el diseño, entrenamiento y simulación de redes neuronales artificiales. Las características generales de esta herramienta  se exponen al final.

  12. Predicción de series de tiempo aplicando redes neuronales artificiales

    OpenAIRE

    Miguel Angel Pérez Chavarría; Hugo Homero Hidalgo Silva; Francisco J. Ocampo Torres

    2002-01-01

    En este trabajo se presentan algoritmos basados en redes neuronales artificiales (RNA) para la predicción de series temporales de las variables oceanográficas Índice de Oscilación del Sur (IOS) y anomalías de temperatura superficial del mar (ATSM). Se realizaron experimentos de predicción utilizando la arquitectura de redes neuronales conocida como redes con respuesta finita al impulso (RNRFI). Se hicieron experimentos variando los diferentes parámetros de la RNRFI, para determinar aquéllos q...

  13. Reconocimiento de patrones proyectivo-invariantes mediante redes neuronales de variable compleja

    OpenAIRE

    Anta González, Pablo J.

    2015-01-01

    El Proyecto desarrolla un sistema de reconocimiento de figuras planas (descritas por sus contornos) en imágenes, robusto a las transformaciones en la forma de las figuras por cambios en el punto de vista (proyectividades). El sistema se basa en la teoría de redes neuronales de variable compleja.

  14. Reconocimiento de patrones proyectivo-invariantes mediante redes neuronales de variable compleja

    OpenAIRE

    Anta González, Pablo J.

    2015-01-01

    El Proyecto desarrolla un sistema de reconocimiento de figuras planas (descritas por sus contornos) en imágenes, robusto a las transformaciones en la forma de las figuras por cambios en el punto de vista (proyectividades). El sistema se basa en la teoría de redes neuronales de variable compleja.

  15. Neuronale Mechanismen der posturalen Kontrolle und der Einfluss von Gleichgewichtstraining

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    Taube W

    2013-01-01

    Full Text Available Für die Aufrechterhaltung des Gleichgewichts findet auf mehreren Stufen des zentralen Nervensystems (ZNS die Integration sensorischer Informationen statt, auf deren Basis motorische Areale adäquate (Kompensations- Reaktionen initiieren. Der vorliegende Überblicksartikel stellt in einem ersten Schritt wichtige Bereiche des ZNS vor, die für die sensomotorische Verarbeitung gleichgewichtsrelevanter Informationen bedeutsam sind. Im Anschluss daran wird ein Überblick über die heutzutage bekannten neuronalen Anpassungsvorgänge an Gleichgewichtstraining gegeben. Dabei wird der Fokus auf Adaptationen auf kortikaler (Motorkortex und Hippokampus und spinaler Ebene gelegt. Zum Schluss werden funktionelle Anpassungen des Gleichgewichtstrainings aufgezeigt, die nicht unmittelbar im Zusammenhang mit der Gleichgewichtsfähigkeit stehen. Diese umfassen die Sensomotorik der Halswirbelsäule, Kraft, Sprungvermögen und Verletzungsprophylaxe.

  16. SISTEMA PARA EL ENTRENAMIENTO PARALELO DE REDES NEURONALES DE PROPAGACIÓN HACIA ATRÁS

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    Alejandro Rosete Suárez

    2006-04-01

    Full Text Available

    El objetivo principal de este trabajo es la exploración del uso de la programación paralela en la instrumentación de redes neuronales, específicamente este artículo trata del uso de tal procesamiento para acelerar la fase de entrenamiento de una red de propagación hacia atrás (BPN. Se describe la implementación y los resultados obtenidos con la red neuronal antes mencionada. El sistema está realizado de manera tal, que el usuario pueda ejecutarlo eficientemente con un amplio rango de aplicaciones neuronales. Para probar el algoritmo paralelo implementado se utilizó una aplicación neuronal real y los resultados obtenidos muestran lo beneficioso de usar el entrenamiento paralelo.

  17. Empleo de redes neuronales artificiales en redes de distribución eléctrica

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    Alexander Cabrera

    2011-10-01

    Full Text Available En el trabajo se muestran las experiencias alcanzadas  en la aplicación de las Redes Neuronales Artificiales para resolver problemas tales como  la predicción de carga y de pérdidas de energía en las líneas y la localización de zonas en falla en circuitos de distribución de la ciudad de Santiago de Cuba.

  18. CODIFICACIÓN DE ESTADOS FUNCIONALES EN REDES NEURONALES BIOLÓGICAS

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    Luis Carrillo-Reid

    2008-01-01

    Full Text Available Un tema central para las neurociencias consiste en descifrar la forma en la cual las redes neuronales biológicas procesan la información y guardan memorias. En 1949 Donald Hebb propuso que la actividad reverberante de pequeños circuitos neuronales llamados "ensambles" funcionaría como el sustrato sobre el cual se construyen las rutas de la memoria. Sin embargo, para poder almacenar cualquier tipo de información compleja, los ensambles neuronales deberían tener la habilidad de organizarse en jerarquías, es decir, pequeñas subrutinas que componen procedimientos más elaborados. Nuestros experimentos muestran que los circuitos con estas capacidades pueden encontrarse en pequeños pedazos de cerebro aislado in vitro y estudiarse con detalle. De esta manera, el cerebro como cualquier sistema puede ser entendido y estudiado de acuerdo con sus elementos básicos y sus reglas de composición; permitiéndonos entender desde la forma en la cual nos comunicamos hasta los hábitos que determinan nuestra vida cotidiana. Los hallazgos experimentales aquí descritos podrían sentar las bases para entender tanto los estados cerebrales normales como los patológicos, con lo cual será posible proponer soluciones para distintas enfermedades neurodegenerativas que tienen un gran impacto en la sociedad.

  19. Subpoblaciones neuronales presentes en el ganglio de la raíz dorsal

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    Marlén Martínez

    2000-09-01

    Full Text Available La función principal de las neuronas del ganglio de la raiz dorsal (GRD es transmitir la información sensorial desde la periferia hasta el sistema nervioso central. Dos clases de células están presentes en el ganglio: las células no neuronales y las neuronales. La heterogeneidad morfológica, fisiológica y bioquimica de la población neuronal permite diferenciarla en subpoblaciones. Morfológicarnente, se distinguen tres tipos neuronales (A, B y C segun el tamaño y las caracteristicas ultraestructurales. Fisiológicamente, hay una relación directa entre el tamaño, el diámetro de las fibras nerviosas y la velocidad con que conducen el impulso nervioso. Finalmente, el uso de marcadores (neuropéptidos, enzimas, receptores, etc. permite realizar una clasificación bioquímica, que es la más utilizada para estudiar la función neuronal. Este articulo revisa la evidencia experimental sobre el tema de la heterogeneidad neuronal del GRD v wresenta una correlación desde el punto de vista bioauimico v fisioló-o ico en los casos en dondé'hay información disponible. El esiudio de subpoblaciones en este ganglio resulta de bastante interés para investigaciones en neurociencias, principalmente en infecciones por virus neurotrópicos, traurnatismos del nervio periférico y el estudio de factores neurotróficos, entre otros.

  20. Redes Neuronales. Una Aplicación para la Predicción de Ozono

    OpenAIRE

    1994-01-01

    Este trabajo presenta una investigación y un análisis sobre las capacidades y limitaciones prácticas de dos modelos neuronales convencionales: el modelo de retropropagación y la memoria asociativa bidireccional (BAM). El fundamento teórico para cada modelo antecede a una aplicación general sobre contaminación ambiental para el pronóstico del nivel de ozono en la ciudad de México. Se realizaron pruebas sobre cada uno de estos modelos, especialmente sobre la BAM. La información necesaria provin...

  1. Identificación de sistemas dinámicos utilizando redes neuronales rbf

    OpenAIRE

    Gachet Páez, Diego; Valverde Gil, Ricardo

    2007-01-01

    La identificación de sistemas complejos y no-lineales ocupa un lugar importante en las arquitecturas de neurocontrol, como por ejemplo el control inverso, control adaptativo directo e indirecto, etc. Es habitual en esos enfoques utilizar redes neuronales “feedforward” con memoria en la entrada (Tapped Delay) o bien redes recurrentes (modelos de Elman o Jordan) entrenadas off-line para capturar la dinámica del sistema (directa o inversa) y utilizarla en el lazo de control. En este artículo pre...

  2. Clasificación de los elementos de una muestra de sangre utilizando Redes Neuronales

    OpenAIRE

    Lanzarini, Laura Cristina; Vargas Camacho, María Teresa; Badrán, Amado F.; De Giusti, Armando Eduardo

    1996-01-01

    El análisis de una muestra de sangre es una de las prácticas más comunes solicitadas por los médicos para un diagnóstico y pronóstico adecuados. Mediante su estudio es posible detectar distintas. patologías que van desde los más simples procesos fisiológicos o inflamatorios a los más complejos procesos neoplásicos (leucemias) y de allí su importancia. El presente trabajo es una aplicación de reconocimiento de patrones utilizando redes neuronales actualmente en desarrollo, que permite clasi...

  3. Predicción de la demanda eléctrica horaria mediante redes neuronales artificiales.

    OpenAIRE

    Mallo González, Carlos

    2004-01-01

    En este trabajo se realiza una introducción a los modelos neuronales y se explora su aplicabilidad en el terreno de la economía cuantitativa como instrumento de modelización y predicción no paramétrica. A tal objeto hemos desarrollado un modelo de Red Neuronal Artificial que posteriormente aplicaríamos en la predicción de la demanda horaria de energía eléctrica con datos reales. Los resultados así obtenidos con los ofrecidos por las metodologías convencionales de ajuste y predicción

  4. Predicción de la demanda eléctrica horaria mediante redes neuronales artificiales.

    Directory of Open Access Journals (Sweden)

    Mallo González, Carlos

    2004-01-01

    Full Text Available En este trabajo se realiza una introducción a los modelos neuronales y se explora su aplicabilidad en el terreno de la economía cuantitativa como instrumento de modelización y predicción no paramétrica. A tal objeto hemos desarrollado un modelo de Red Neuronal Artificial que posteriormente aplicaríamos en la predicción de la demanda horaria de energía eléctrica con datos reales. Los resultados así obtenidos con los ofrecidos por las metodologías convencionales de ajuste y predicción

  5. Lógica borrosa y redes neuronales artificiales aplicadas a las Ciencias Sociales

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    José A. Amozurrutia

    2006-01-01

    Full Text Available Se presenta una introducción a la Lógica Borrosa y a las Redes Neuronales Artificiales como dos teorías que enriquecen la perspectiva analítica de la sociología. Su aplicación abarca desde el modelado de sistemas de interacción y sistemas de organizaciones, hasta la simulación de agentes y fenómenos sociales. Se parte de una postura crítica hacia la mirada clásica del sociólogo que descansa en las teorías de la probabilidad y la estadística para formalizar sus razonamientos. Se toma en cuenta la complejidad de objetos de estudio derivados de una observación de segundo orden, es decir a partir de una reflexión del investigador que se observa investigar, y sostengo que, mediante la lógica borrosa y varios componentes derivados de la teoría y de los modelos de redes neuronales artificiales, es posible establecer nuevas formas de «comprensión/explicación» de fenómenos sociales.

  6. Uso de redes neuronales para optimizar simulaciones en un modelo de flujo de pasajeros en aeropuertos

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    Joaquin Pacheco

    2002-01-01

    Full Text Available Uno de nuestros principales objetivos es el diseño de métodos de aprendizaje de redes neuronales que sean rápidos a la vez que den buenas soluciones o vectores de pesos. Una de las aplicaciones donde se necesita un método rápido (on-line de aprendizaje de las redes neuronales es en la optimización de simulaciones. En este informe se proponen un sencillo algoritmo de búsqueda local para optimizar simulaciones, con una aplicación a un interesante modelo de flujo de pasajeros en un aeropuerto. En este problema se contemplan la minimización del tiempo de recorrido de los pasajeros con una adecuada distribución de los recursos. Este método incorpora el uso de una red neuronal para acelerar la optimización. Se va a comparar el efecto del uso de los pesos obtenidos por diferentes métodos de aprendizaje de la red neuronal, propuestos recientemente para este fin.

  7. LAS REDES NEURONALES Y LA EVALUACIÓN DEL RIESGO DE CRÉDITO

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    Fredy Ocaris Pérez Ramírez

    2007-01-01

    Full Text Available A pesar del escepticismo del mundo académico sobre los avances de la inteligencia artificial, las redes neuronales han abierto un campo de exploración bursátil que aún tiene mucho por investigar. Atendiendo a las ventajas del uso de las redes neuronales artificiales (ANN, por sus siglas en inglés y a su capacidad para estimar modelos no lineales, en este artículo se muestra la aplicación de las redes neuronales a la cuantificación del riesgo de crédito. Además, se hace el desarrollo teórico de los fundamentos básicos de las redes neuronales. Para presentar las metodologías de medición de riesgo de crédito basados en redes neuronales, y aplicarlas a la base de datos de una cartera comercial, fue necesario elaborar un análisis exploratorio de cada una de las variables e investigar la correlación entre ellas. El objetivo del análisis es encontrar algunas relaciones para grupos determinados de la población, de acuerdo con sus características particulares. Por tanto, se cruzan variables de cada cliente, del crédito y del comportamiento contra la variable default (fallidos y no fallidos. Variable que establece un procedimiento de clasificación, y permite determinar las ponderaciones necesarias y, además, establece la probabilidad de fallido.In spite of the skepticism of the academic world on the advances of artificial intelligence, the neuronal networks have opened up a field of stock-exchange exploration that has still so much to research. Upon expounding the advantages of the usage of artificial neuronal network (ANN and its capacity to estimate nonlinear models this article shows the application of the neuronal networks on the quantification of the credit risk. Furthermore, the article carries out a theoretical development of the basic foundations of neuronal networks. In order to present the methodologies of measurements of credit risk, based upon the neuronal networks and to apply the to the data base of a commercial

  8. Lógica borrosa y redes neuronales artificiales aplicadas a las Ciencias Sociales

    OpenAIRE

    José A. Amozurrutia

    2006-01-01

    Se presenta una introducción a la Lógica Borrosa y a las Redes Neuronales Artificiales como dos teorías que enriquecen la perspectiva analítica de la sociología. Su aplicación abarca desde el modelado de sistemas de interacción y sistemas de organizaciones, hasta la simulación de agentes y fenómenos sociales. Se parte de una postura crítica hacia la mirada clásica del sociólogo que descansa en las teorías de la probabilidad y la estadística para formalizar sus razonamientos. Se toma en cuenta...

  9. Aproximación de los problemas de optimización por redes neuronales artificiales

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    Caballero Fernandez, Rafael

    2001-01-01

    Full Text Available En el presente artículo, pretendemos aportar un conocimiento de cómo puede aproximarse un problema de optimización mediante la dinámica de las redes neuronales artificiales, específicamente mediante las redes de tipo Hopfield. La elección de este tipo de redes radica en el objetivo que nos hemos propuesto en el presente trabajo, la resolución on-line de los problemas de optimización lineal, cuadrática, no lineal y combinatoria. Construimos, desarrollamos y justificamos formalmente una metodología integral que permite recoger los distintos tipos de problemas de optimización y construir una red neuronal de tipo Hopfield que los resuelva de una manera computacionalmente adecuada y formalmente correcta, comparando los resultados obtenidos con los publicados más recientemente.

  10. Modelado del indice de precios al consumidor usando un modelo hibrido basado en redes neuronales artificiales

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    JUAN DAVID VELÁSQUEZ HENAO

    2005-01-01

    Full Text Available Un nuevo modelo híbrido es propuesto para pronosticar el índice colombiano de precios al consumidor. Este es basado en una descomposición estructural de la serie temporal con el objetivo de remover cualquier patrón fácilmente detectable en los datos, y en el uso de un perceptron multicapa para modelar las relaciones ocultas en la serie de tiempo. Los resultados superan las aproximaciones clásicas basadas en la aproximación de Box y Jenkins, y los modelos convencionales de Redes Neuronales, e incentivan el estudio de este tipo de aproximación híbrida para modelar otras series temporales.

  11. SIMULACIÓN MEDIANTE REDES NEURONALES DEL ENFRIAMIENTO DEL ARCO ELÉCTRICO EN DISYUNTORES DE ALTA TENSIÓN

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    ALEXANDRO BRYANTSEVICH

    2010-07-01

    Full Text Available La formulación de los modelos analíticos de enfriamiento de los arcos eléctricos en disyuntores de alta tensión todavía presenta muchas dificultades, y requiere nuevas hipótesis que los simplifique sustancialmente en relación con la realidad. Desde un punto de vista técnico, la aplicación de las redes neuronales podría ser una fuente objetiva para la simulación del enfriamiento del arco eléctrico, y para obtener resultados más cercanos a las propiedades reales del interruptor. En este trabajo se describe la problemática del desarrollo de modelos analíticos vinculados al enfriamiento del arco eléctrico de disyuntores de alta tensión mediante redes neuronales.

  12. Neural correlates of working memory deficits in schizophrenic patients. Ways to establish neurocognitive endophenotypes of psychiatric disorders; Neuronale Korrelate gestoerter Arbeitsgedaechtnisfunktionen bei schizophrenen Patienten. Ansaetze zur Etablierung neurokognitiver Endophaenotypen psychiatrischer Erkrankungen

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    Gruber, O. [Universitaet des Saarlandes, Klinik fuer Psychiatrie und Psychotherapie, Homburg (Saar) (Germany); Max-Planck-Institut fuer Kognitions- und Neurowissenschaften, Leipzig (Germany); Gruber, E.; Falkai, P. [Universitaet des Saarlandes, Klinik fuer Psychiatrie und Psychotherapie, Homburg (Saar) (Germany)

    2005-02-01

    This article briefly reviews some methodological limitations of functional neuroimaging studies in psychiatric patients. We argue that the investigation of the neural substrates of cognitive deficits in psychiatric disorders requires a combination of functional neuroimaging studies in healthy subjects with corresponding behavioral experiments in patients. In order to exemplify this methodological approach we review recent findings regarding the functional neuroanatomy of distinct components of human working memory and provide evidence for selective dysfunctions of cortical networks that underlie specific working memory deficits in schizophrenia. This identification of subgroups of schizophrenic patients according to neurocognitive parameters may facilitate the establishment of behavioral and neurophysiological endophenotypes and the development of a neurobiological classification of psychiatric disorders. (orig.) [German] Dieser Beitrag befasst sich mit einigen methodischen Problemen funktionell-bildgebender Studien mit psychiatrischen Patienten, aufgrund derer die Untersuchung der neuronalen Korrelate kognitiver Defizite bei psychiatrischen Erkrankungen einer Kombination funktionell-bildgebender Studien bei gesunden Normalprobanden mit Verhaltensuntersuchungen bei Patienten bedarf. Dieser methodische Ansatz wird am Beispiel von Arbeitsgedaechtnisfunktionen erlaeutert, wobei zunaechst neuere Erkenntnisse zur funktionellen Neuroanatomie verschiedener Komponenten des menschlichen Arbeitsgedaechtnisses referiert werden. Anschliessend werden bei schizophrenen Patienten erhobene Befunde vorgestellt, die auf spezifische Stoerungen der funktionellen Integritaet neuronaler Netzwerke mit Arbeitsgedaechtnisfunktionen hinweisen. Die damit verbundene Identifikation von Subgruppen schizophrener Patienten koennte zur Etablierung verhaltensneurophysiologisch definierter Endophaenotypen psychiatrischer Stoerungsbilder fuehren und die Entwicklung einer neurowissenschaftlich

  13. Redes neuronales artificiales en la estimación de la evapotranspiración de referencia Artificial neural networks in the estimation of reference evapotranspiration

    Directory of Open Access Journals (Sweden)

    Rocío Cervantes-Osornio

    2011-06-01

    Full Text Available Las redes neuronales artificiales representan un vasto campo de investigación, puesto que han demostrado tener aplicación en varios campos de la ciencia, su capacidad de lidiar con no linealidades en diversos fenómenos, y los diferentes trabajos realizados en la estimación y/o pronóstico para predecir variables climáticas, que inciden directa e indirectamente en la evapotranspiración de referencia y la propia evapotranspiración ha originado el desarrollo de este trabajo. El objetivo fue presentar una revisión de literatura sobre redes neuronales artificiales, para la estimación de la evapotranspiración de referencia y variables relacionadas, que incluye: la teoría y fundamentos de las redes neuronales artificiales y el algoritmo backpropagation; algunas similitudes y diferencias entre los modelos estadísticos tradicionales y las redes neuronales artificiales; aplicaciones de las redes neuronales artificiales en la estimación de la evapotranspiración de referencia; y variables que se asocian con las perspectivas de las redes neuronales artificiales en la predicción de variables agroclimáticas. Las redes neuronales artificiales estáticas multicapa, son hasta ahora las más comunes en la estimación de evapotranspiración de referencia, y se vislumbra un cambio en la tendencia de aplicar redes neuronales artificiales de tipo dinámico.Artificial neural networks represent a vast research field, since they have demonstrated application in various fields of science. Its ability to cope with nonlinearities in several different phenomena and work in the estimation or forecast meteorological variables, which act directly and indirectly in reference evapotranspiration and actual evapotranspiration, have led to this work development. The aim was to present a literature review on artificial neural networks for reference evapotranspiration estimating and related variables, including: theory and artificial neural networks foundations and

  14. Redes neuronales analógicas para la computación paralela masiva de señales en tiempo real

    OpenAIRE

    Cancelo, Gustavo Indalecio Eugenio

    1996-01-01

    En esta tesis se realiza un estudio sobre las posibilidades de la electrónica para modelar unidades y sistemas neuronales. En particular, se proponen implementaciones de redes neuronales analógicas. La primera a nivel microelectrónico, comprende el diseño de una neurona computacional con función de activación gaussiana. Primeramente, se demostrará que las unidades computacionales con función de activación gaussiana forman una base para aproximadores universales de funciones continuas y discon...

  15. Redes neuronales analógicas para la computación paralela masiva de señales en tiempo real

    OpenAIRE

    Cancelo, Gustavo Indalecio Eugenio

    1996-01-01

    En esta tesis se realiza un estudio sobre las posibilidades de la electrónica para modelar unidades y sistemas neuronales. En particular, se proponen implementaciones de redes neuronales analógicas. La primera a nivel microelectrónico, comprende el diseño de una neurona computacional con función de activación gaussiana. Primeramente, se demostrará que las unidades computacionales con función de activación gaussiana forman una base para aproximadores universales de funciones continuas y discon...

  16. Evaluación de las Redes Neuronales Artificiales Perceptron Multicapa y Fuzzy-Artmap en la Clasificación de Imágenes Satelitales

    Directory of Open Access Journals (Sweden)

    Guillermo Antonio Toro Bayona

    2012-06-01

    Full Text Available Este documento reporta una comparación cuantitativa y cualitativa del desempeño de las redes neuronales artificiales Perceptron Multicapa y Fuzzy-Artmap para clasificación de coberturas del suelo a partir de imágenes satelitales multi-espectrales. Se describen parámetros y condiciones que ayudan a producir imágenes clasificadas con buena exactitud temática. Adicionalmente, se hace una comparación entre los dos modelos de redes neuronales que describen sus ventajas y desventajas.

  17. Realidad Virtual Acústica: El Abordaje de las Redes Neuronales Artificiales

    Directory of Open Access Journals (Sweden)

    Jose Francisco Lucio

    2015-05-01

    Full Text Available En este trabajo se presenta un nuevo abordaje para obtener las respuestas impulsivas biauriculares (BIRs para un sistema de aurilización utilizando un conjunto de redes neuronales artificiales (RNAs. El método propuesto es capaz de reconstruir las respuestas impulsivas asociadas a la cabeza humana (HRIRs por medio de modificación espectral y de interpolación espacial. Para poder cubrir todo el espacio auditivo de recepción, sin aumentar la complejidad de la arquitectura de la red, una estructura con varias RNAs (conjunto fue adoptada, donde cada red opera en una región específica del espacio (gomo. El error de modelaje en el dominio de la frecuencia es investigado considerando la naturaleza logarítmica de la audición humana. A través de la metodología propuesta se obtuvo un ahorro del tiempo de procesamiento computacional de aproximadamente 62% en relación al método tradicional de procesamiento de señales utilizado para aurilización. La aplicabilidad del nuevo método en sistemas de aurilización es reforzada mediante un análisis comparativo de los resultados, que incluyen la generación de las BIRs y el cálculo de un parámetro acústico biauricular (IACF, los cuales muestran errores con magnitudes reducidas.

  18. Estrategias para el entrenamiento de redes neuronales de números difusos

    Directory of Open Access Journals (Sweden)

    Edwin Villarreal López

    2014-04-01

    Full Text Available El propósito de este artículo es presentar estrategias generales de entrenamiento para redes neuronales de números difusos utilizadas en el aprendizaje de sistemas a partir de información lingüística. Se exponen brevemente las principales tendencias en el entrenamiento de este tipo de sistemas y con base en ellas se proponen nuevas estrategias. La primera de ellas se basa en la retro-propagación del error cuadrático medio en todos los a-cortes para pesos crisp. La segunda hace uso de un algoritmo genético con codificación real para redes con pesos crisp. La tercera consiste en la retro-propagación del error en el valor promedio y la ambigüedad en todos los a-cortes para pesos difusos. Por último, se presenta una basada en la retro-propagación de una medida difusa del error para redes con pesos difusos. Se realiza una etapa experimental en la que se implementan los algoritmos desarrollados junto con algunos de los más representativos reportados en el estado del arte, permitiendo identificar para qué conjuntos de datos particulares resulta útil cada una de las estrategias. Finalmente, se aplican dichas estrategias para la implementación de un sistema de evaluación de impacto ambiental en vertederos.

  19. Geothermics and neural networks: A first approach; Geotermia y redes neuronales: Una primera aproximacion

    Energy Technology Data Exchange (ETDEWEB)

    Flores Armenta, Magaly del Carmen [Gerencia de Proyectos Geotermoelectricos de la Comision Federal de Electricidad, Morelia (Mexico); Barragan Orbe, Carlos [Comision Federal de Electricidad, Mexico, D. F. (Mexico)

    1995-09-01

    Neural networks have been hailed as the greatest technological advance since the transistor. They are predicted to be a common household item by the year 2000. This new form of machine intelligence is able to solve problems, without using rules of math, they only need examples to learn from. The first geothermal problem, to solve with this powerful tool, is the prediction of liquid and steam production, as well as the well`s termination in Los Humeros, Puebla, Mexico. The first attempt shows the learning`s capacity of the developed model, and its precision on the predictions that were done. [Espanol] Las redes neuronales se consideran como uno de los mas grandes avances tecnologicos desde la invencion del transistor, de tal forma que se perfilan como una de las herramientas mas comunes del ano 2000. Esta nueva forma de inteligencia artificial es capaz de resolver problemas sin utilizar reglas matematicas, solamente requiere de ejemplos para aprender de ellos. El primer problema geotermico, atacado con esta poderosa herramienta, es la prediccion de las producciones de vapor y liquido, asi como la terminacion de pozos en el campo geotermico de Los Humeros, Puebla, Mexico. Los primeros intentos demuestran la capacidad de aprender del modelo desarrollado, y su certeza en las predicciones realizadas.

  20. Redes neuronales recurrentes para el análisis de secuencias

    Directory of Open Access Journals (Sweden)

    Sain Salazar Martínez

    2011-10-01

    Full Text Available En este trabajo se realiza un estudio de la importancia de las redes recurrentes y de su naturaleza en análisis de secuenciales o señales donde es muy importante tener en cuenta el pasado o el futuro. Se muestra la fortaleza de estos métodos para analizar secuencias de tamaño variable, por su despliegue en el tiempo en función del tamaño de la entrada.  Se construyen específicamente redes neuronales recurrentes bidireccionales, como una especificación de redes recurrentes, mostrando la potencialidad de las mismas en sistemas no causales, donde las entradas pueden depender de entradas de tiempos pasados y futuros. Además se desarrolla una plataforma para implementar redes recurrentes dinámicas, con algoritmo de aprendizaje Backpropagation Trough Time; que permiten desarrollar redes para cualquier problema donde las entradas son secuencias analizadas en el tiempo y la salida son otras secuencias o simplemente descriptores de funciones o propiedades de las mismas.

  1. Un método cuantitativo para analizar redes neuronales marcadas por histoquímica para acetilcolinesterasa

    Directory of Open Access Journals (Sweden)

    Luz H. Camargo

    2004-12-01

    Full Text Available El análisis cualitativo de las redes neuronales por histoquímica enzimática se usa comúnmente en los estudios morfológicos tradicionales. Una limitante de este tipo de estudios consiste en la dificultad de obtener resultados cuantitativos. Este artículo presenta dos técnicas originales de procesamiento de imágenes para realizar estudios cuantitativos y un análisis comparativo entre ellas. Los resultados preliminares presentados permiten verificar la utilidad de la metodología aplicada.

  2. Redes neuronales artificiales : Aplicaciones en minería de datos y procesamiento de señales temporales

    OpenAIRE

    Lanzarini, Laura Cristina; Hasperué, Waldo; Estrebou, César Armando; Ronchetti, Franco; Villa Monte, Augusto; Aquino, Germán Osvaldo; Quiroga, Facundo; Rojas, Luis; Jimbo Santana, Patricia

    2015-01-01

    Esta línea de investigación se centra en el estudio y desarrollo de Sistemas Inteligentes basados en Redes Neuronales Artificiales aplicadas al Procesamiento de Señales Temporales y a la Minería de Datos. Con respecto al Procesamiento de Señales Temporales, el énfasis está puesto en el reconocimiento de acciones humanas con el objetivo de mejorar la interfaz hombre/máquina y en el reconocimiento de señales oculares como herramienta de ayuda al diagnóstico médico semi-automático de alteraci...

  3. Calibración automática de modelos neuronales para su uso en circuitos híbridos

    OpenAIRE

    Reyes Sánchez, Manuel

    2016-01-01

    Este proyecto tiene como objetivo la calibración automática en tiempo real de modelos neuronales para su uso en circuitos híbridos, compuestos por neuronas vivas procedentes de sistemas biológicos y neuronas software. Estas conexiones se realizan por medio de sinapsis artificiales bidireccionales entre las neuronas vivas y las neuronas artificiales. La calibración automática supone un paso importante en este tipo de conexiones dada la corta vida útil durante la cual pueden util...

  4. Análisis del error en redes neuronales: Corrección de los datos y distribuciones no balanceadas

    OpenAIRE

    Alejo Eleuterio, Roberto

    2010-01-01

    El problema del desbalance de las clases aparece cuando existen muchos más elementos de una o algunas clases, que de la otra u otras clases (dos o múltiples clases). Esta desproporción en el tamaño de las diferentes clases en un mismo conjunto de datos, puede ocasionar una disminución en la efectividad del clasificación sobre las clases menos representadas. En el caso específico de las redes neuronales artificiales, el desbalance de las clases ocasiona lentitud en la convergencia de las clase...

  5. La evolución del aprendizaje: más allá de las redes neuronales

    OpenAIRE

    Alma Dzib Goodin

    2013-01-01

    El tema del aprendizaje, ha sido ampliamente estudiado desde distintas perspectivas y en años recientes gracias al avance tecnológico, ha sido parte de diversos estudios con redes neuronales artificiales, las cuales tienen un punto de engranaje con la evolución de sistemas complejos. El desarrollo de éstas ha permitido comprender cómo la naturaleza ha dotado a los sistemas con la habilidad para adaptarse al medio, que es la línea que permite la pervivencia de las especies, lo cual puede ser c...

  6. Modelo director no paramétrico de amortiguador magneto-reológico basado en redes neuronales

    OpenAIRE

    Muñoz Águila, Felipe Borja

    2012-01-01

    El objetivo de este Proyecto Fin de Carrera es el de desarrollar un modelo de amortiguador magneto-reológico basado en redes neuronales que prediga su comportamiento no lineal e hiterético. Se pretende encontrar el algoritmo adecuado para relacionar amplitud, frecuencia y corriente con la fuerza; para ello se dispone de una serie de datos de partida con los que se procederá de la siguiente forma: · Análisis de los datos obtenidos en los ensayos experimentales. · Diseño de la red neuronal arti...

  7. GENOMIQUE ET LIPIDES Génomique et métabolisme des lipides des plantes

    Directory of Open Access Journals (Sweden)

    Delseny Michel

    2002-03-01

    Full Text Available Il existe dans les bases de données publiques une énorme quantité de séquences d’ADN dérivées de plantes, et notamment la séquence complète du génome d’Arabidopsis thaliana, une plante modèle pour les oléagineux, proche parente du colza. Ces données constituent une ressource importante non seulement pour la compréhension de métabolisme lipidique et de sa régulation, mais aussi pour la sélection et le développement de variétés nouvelles d’oléagineux produisant davantage d’huiles ou des huiles de composition nouvelle. Cette abondance de séquences peut être exploitée, en utilisant les recherches d’homologies, pour identifier les gènes, pour obtenir des informations sur leur fonction, comme pour repérer des gènes candidats codant des fonctions nouvelles. L’analyse de ces bases de données a révélé que la majeure partie des gènes codant des enzymes impliquées dans le métabolisme lipidique appartient à des petites familles multigéniques, reflétant la diversification des fonctions des isoformes. Une analyse du catalogue des ADNc séquencés en aveugle reflète les niveaux d’expression des différents gènes et fournit un aperçu des régulations des flux au travers des voies métaboliques conduisant à la biosynthèse des lipides de réserve. La disponibilité de mutants et de lignées transgéniques d’Arabidopsis et le développement de puces à ADN qui permettent l’analyse simultanée de plusieurs milliers de gènes conduiront à une meilleure compréhension des facteurs qui régulent le métabolisme des huiles dans les graines. Une telle connaissance facilitera la manipulation de la composition des huiles et des quantités produites dans les graines.

  8. REDES NEURONALES PARA LA PREDICCIÓN DE PRECIOS EN EL SECTOR LÁCTEO ARGENTINO

    Directory of Open Access Journals (Sweden)

    Jimena Vicentin Masaro

    2017-01-01

    Full Text Available Realizar pronósticos sobre precios resulta muy impo rtante no sólo para la toma de decisiones de un sector productivo, sino también pa ra la elaboración de políticas públicas; ya que permite reducir la incertidumbre de los posi bles escenarios a los que se puede enfrentar dicho sector. Los precios son una variabl e clave en el análisis sectorial, y contar con estimaciones confiables de los mismos no es tar ea fácil, mucho menos en países con alta volatilidad como lo es Argentina. A partir de una revisión de antecedentes, se ha encontrado que los métodos lineales multivariados h an liderado en esta área de predicciones económicas. Sin embargo, trabajos reci entes empiezan a implementar mecanismos no lineales que, mediante un diseño senc illo, han logrado una performance predictiva competentes a las primeras, al menos en el área económica. Dentro de estos mecanismos no lineales se encuentran las redes neur onales. El presente trabajo tiene como objetivo ajustar un modelo de red neuronal par a realizar predicciones aplicadas al sector lácteo argentino, y comparar los resultados con las predicciones que arroja un modelo multivariado de series de tiempo. Sobre una base de datos mensual disponible para variables del sector desde 2000 a 2012, se log ra obtener una red con tres capas, cuya capa interna tiene sólo dos nodos, para predecir lo s precios mensuales pagados a los productores tamberos. Los resultados obtenidos mues tran que mediante una estructura sencilla y parsimoniosa de redes neuronales, pueden obtenerse mejores resultados predictivos respecto a alternativas más tradicional es de series de tiempo.

  9. Estimación de la resistencia a la penetración de suelos usando redes neuronales artificiales

    Directory of Open Access Journals (Sweden)

    Nidia Johana Valdés Holguín

    2011-07-01

    Full Text Available Las redes neuronales artificiales, simuladoras del proceso de aprendizaje de las neuronas biológicas, han sido utilizadas con éxito en el cálculo de parámetros en diversos problemas de ingeniería en que las variables involucradas tienen una alta relación no lineal entre sí y la modelación no permite representar el problema mediante una función matemática de fácil deducción. En la ciencia del suelo la predicción de algunas propiedades involucra diversas variables que hacen de su estimación por medio de modelos matemáticos un proceso complejo, y trasladan la solución del problema al campo de la inteligencia artificial. En el presente artículo se reporta la elaboración de redes neuronales artificiales para la estimación de la resistencia a la penetración a diferentes profundidades de un suelo; se consideran como variables influyentes el contenido de humedad, la densidad, la carga estática y la presión de inflado. Los resultados muestran una mejor estimación para profundidades entre 20 cm y 30 cm.

  10. Redes Neuronales y su aplicación predictiva en la Bolsa de Valores española

    Directory of Open Access Journals (Sweden)

    Ibarra Alfaraz, J.A.

    1999-01-01

    Full Text Available Este trabajo recoge una visión general de las redes neuronales y su tendencia en la investigación actual. Además de las aplicaciones conocidas donde las redes neuronales han demostrado su aplicabilidad se abren nuevos campos de investigación. En nuestro caso, nos centraremos en el área económica y más concretamente en la financiera. Esta metodología se aplica al caso concreto del análisis predictivo de la bolsa de valores, concretamente se utiliza el índice del mercado continuo de la bolsa española, Ibex-35, y los recientemente aparecidos índices sectoriales del Ibex: Servicios, Financiero, Utilities y Complementario. La red neuronal utilizada, Perceptron, ha sido entrenada con los datos reales procedentes de la bolsa de valores utilizando diferentes periodos de tiempo y efectuando cambios en los parámetros que condicionan la capacidad predictiva de la red. Los resultado se han contrastado con los obtenidos en otros trabajos empíricos realizados con metodologías clásicas.

  11. Estimación de la resistencia a la penetración de suelos usando redes neuronales artificiales

    Directory of Open Access Journals (Sweden)

    Valdés Holguín Nidia Johana

    2011-09-01

    Full Text Available Las redes neuronales artificiales, simuladoras del proceso de aprendizaje de las neuronas biológicas, han sido utilizadas con éxito en el cálculo de parámetros en diversos problemas de ingeniería en que las variables involucradas tienen una alta relación no lineal entre sí y la modelación no permite representar el problema mediante una función matemática de fácil deducción. En la ciencia del suelo la predicción de algunas propiedades involucra diversas variables que hacen de su estimación por medio de modelos matemáticos un proceso complejo, y trasladan la solución del problema al campo de la inteligencia artificial. En el presente articulo se reporta la elaboración de redes neuronales artificiales para la estimación de la resistencia a la penetración a diferentes profundidades de un suelo; se consideran como variables infiuyentes el contenido de humedad, la densidad, la carga estãtica y la presión de infiado. Los resultados muestran una mejor estimación para profundidades entre 20 cm y 30 cm.

  12. Histamine H3-receptor isoforms.

    Science.gov (United States)

    Bakker, R A

    2004-10-01

    Increasing evidence supports a role for HA as a neurotransmitter and neuromodulator in various brain functions, including emotion, cognition, and feeding. The recent cloning of the histamine H3 receptor allowed for the subsequent cloning of a variety of H3 receptor isoforms from different species as well as the H4 receptor. As a result a wide variety of H3-receptor isoforms are now known that display differential brain expression patterns and signalling properties. These recent discoveries are discussed in view of the growing interest of the H3 receptor as a target for the development of potential therapeutics.

  13. Pronóstico de los índices accionarios DAX y S&P 500 con redes neuronales diferenciales

    Directory of Open Access Journals (Sweden)

    Francisco Ortiz Arango

    2013-01-01

    Full Text Available En este trabajo se utiliza una red neuronal diferencial (RND para describir las series de valores de cierre diarios de los índices accionarios DAX de Alemania y S&P 500 de Estados Unidos entre el periodo del 3 de julio de 2000 y el 13 de enero de 2012. Con la RND se lleva a cabo el pronóstico de los valores de cierre diarios de esos índices durante un periodo de cuatro semanas (del 16 de enero al 10 de febrero de 2012. Los resultados obtenidos confirman el hecho de que las redes neuronales diferenciales pueden constituirse en una de las herramientas más poderosas y precisas para poder pronosticar valores futuros de activos financieros.

  14. Órdenes de flujo, tasa de interés y tasa de cambio nominal: un ejemplo de redes neuronales para Colombia 2005

    OpenAIRE

    Salcedo Parra, Octavio José; Aguilera Prado, Marco

    2006-01-01

    El presente documento muestra un modelo de Redes Neuronales Artificiales, RNA, para el pronóstico de la tasa de cambio nominal en Colombia, que incluye órdenes de flujo y el diferencial de las tasas de interés como variables de entrada al modelo, hallando relaciones no lineales entre las mismas. Adicionalmente se plantean algunas conclusiones metodológicas a partir de los tradicionales esquemas econométricos para el manejo de las series de tiempo.

  15. ANÁLISIS Y PREDICCIÓN DE LA SERIE DE TIEMPO DEL PRECIO EXTERNO DEL CAFÉ COLOMBIANO UTILIZANDO REDES NEURONALES ARTIFICIALES

    Directory of Open Access Journals (Sweden)

    Ismael García Martín

    2003-12-01

    Full Text Available En el presente artículo se busca diseñar un modelo no lineal para el análisis y predicción de la serie de tiempo del precio externo del café colombiano utilizando Redes Neuronales Artificiales. El objetivo escomparar los alcances y limitaciones de este modelo frente a un modelo clásico de predicción lineal ARIMA.

  16. Diagnóstico automático del síndrome coronario agudo utilizando un sistema multiagente basado en redes neuronales

    Directory of Open Access Journals (Sweden)

    John Jaime Sprockel Díaz

    2017-05-01

    Conclusiones: Es posible desarrollar una herramienta para el diagnóstico automático del síndrome coronario agudo a partir de un sistema multiagente que ensamble la disposición tomada por un conjunto de redes neuronales artificiales, cuyo rendimiento permite su consideración para su implementación dentro de un sistema de soporte a las decisiones clínicas.

  17. Le diagnostic des encéphalopathies spongiformes chez les ruminants

    OpenAIRE

    2004-01-01

    Le diagnostic des Encéphalopathies Spongiformes Transmissibles (EST) des ruminants est aujourd’hui basé sur la mise en évidence de l’isoforme pathologique, résistante à la protéinase K, de la protéine PrP, la PrPres. La PrPres peut être mise en évidence in situ, à l’aide de techniques immunohistochimiques ou, bien après extraction et traitement à la protéinase K, par des techniques immunochimiques (western blot, Enzyme Linked ImmunoSorbent Assay). Si le diagnostic de routine des encéphalopath...

  18. Des tuiles, des toits et des couleurs

    OpenAIRE

    Bonnot, Thierry

    2007-01-01

    La Bourgogne est une des régions françaises dont l’image de marque est le plus imprégnée par la notion de tradition. Elle est présentée comme un terroir où il fait bon vivre, où la gastronomie demeure à travers les siècles une valeur de base, où les paysages sont majestueux et où la gloire passée est sans cesse rappelée par de somptueux monuments. Parmi quelques emblèmes, l’hôtel-Dieu de Beaune et ses toitures de tuiles colorées sont très souvent mobilisés pour représenter ces valeurs « tradi...

  19. Inference of Isoforms from Short Sequence Reads

    Science.gov (United States)

    Feng, Jianxing; Li, Wei; Jiang, Tao

    Due to alternative splicing events in eukaryotic species, the identification of mRNA isoforms (or splicing variants) is a difficult problem. Traditional experimental methods for this purpose are time consuming and cost ineffective. The emerging RNA-Seq technology provides a possible effective method to address this problem. Although the advantages of RNA-Seq over traditional methods in transcriptome analysis have been confirmed by many studies, the inference of isoforms from millions of short sequence reads (e.g., Illumina/Solexa reads) has remained computationally challenging. In this work, we propose a method to calculate the expression levels of isoforms and infer isoforms from short RNA-Seq reads using exon-intron boundary, transcription start site (TSS) and poly-A site (PAS) information. We first formulate the relationship among exons, isoforms, and single-end reads as a convex quadratic program, and then use an efficient algorithm (called IsoInfer) to search for isoforms. IsoInfer can calculate the expression levels of isoforms accurately if all the isoforms are known and infer novel isoforms from scratch. Our experimental tests on known mouse isoforms with both simulated expression levels and reads demonstrate that IsoInfer is able to calculate the expression levels of isoforms with an accuracy comparable to the state-of-the-art statistical method and a 60 times faster speed. Moreover, our tests on both simulated and real reads show that it achieves a good precision and sensitivity in inferring isoforms when given accurate exon-intron boundary, TSS and PAS information, especially for isoforms whose expression levels are significantly high.

  20. Identificación del estado de madurez de las frutas con redes neuronales artificiales, una revisión

    Directory of Open Access Journals (Sweden)

    Gustavo Andrés Figueredo-Ávila

    2016-01-01

    Full Text Available La aplicación de las Redes Neuronales Artificiales (RNA y de la visión artificial tiene cada vez más acogida en la industria de productos alimenticios; estas técnicas priorizan la clasificación, el reconocimiento de patrones y la predicción de las cosechas y de los cambios físicos de sus productos. En este artículo se define el concepto de red neuronal y se describen sus principales características y modelos, y, por otro lado, se define el concepto de procesamiento de imágenes digitales y las diferentes etapas que lo componen. Complementariamente, se exponen las generalidades de la inspección de frutas (enfocada en Colombia y sus técnicas. Finalmente, se especifican diferentes trabajos en los que se aplicaron técnicas de RNA y visión artificial en el campo de los productos alimenticios, dispuestos por áreas de  aplicación, y se identifica de manera concluyente el impacto que estas dos técnicas tienen para la clasificación, el reconocimiento de patrones y la predicción en el sector de productos alimenticios.

  1. Isolation and characterization of patatin isoforms

    NARCIS (Netherlands)

    Pots, A.M.; Gruppen, H.; Hessing, M.; Boekel, M.A.J.S. van; Voragen, A.G.J.

    1999-01-01

    Patatin has, so far, been considered a homogeneous group of proteins. A comparison of the isoforms in terms of structural properties or stability has not been reported. A method to obtain various isoform fractions as well as a comparison of the physicochemical properties of these pools is presented.

  2. EXPLORACIÓN CON REDES NEURONALES ARTIFICIALES PARA ESTIMAR LA RESISTENCIA A LA COMPRESIÓN, EN CONCRETOS FIBROREFORZADOS CON ACERO

    Directory of Open Access Journals (Sweden)

    Luis Octavio González Salcedo

    2012-01-01

    Full Text Available En diseño y construcción de estructuras de concreto, la resistencia a la compresión a 28 días de curado es la especificación de control de estabilidad de la obra. La inclusión de fibras como reforzamiento de la matriz cementicia permite una ganancia en sus propiedades, además de obtener un material de alto desempeño. En las normativas, se plantean formulaciones predictivas de la resistencia a la compresión basadas en unos pocos parámetros de composición del concreto, tales como la relación agua/cemento y el contenido de cemento Portland. Por otra parte, también se han planteado métodos de diseños de concreto para definir la ponderación de sus materiales componentes, teniendo como referencia la resistencia a la compresión del concreto simple. Además, las redes neuronales artificiales, como un símil de las neuronas biológicas, han sido utilizadas como herramientas de predicción de la resistencia a la compresión en el concreto, también con referencia al concreto simple, sin reforzamiento con fibras. Los antecedentes en este uso muestran que es interesante desarrollar aplicaciones en los concretos reforzados con fibras. En el presente trabajo se elaboraron redes neuronales artificiales para predecir la resistencia a la compresión en concretos reforzados con fibras de acero. Los resultados de los indicadores de desempeño mostraron que las redes neuronales artificiales elaboradas pueden realizar una aproximación adecuada al valor real de la propiedad mecánica.

  3. Modelado y control basado en redes neuronales artificiales de una planta piloto de desalinización de agua de mar de ósmosis inversa

    OpenAIRE

    Carrasco Banda, Neil Neizer

    2016-01-01

    En este trabajo de tesis se parte de la hipótesis de que: es posible aumentar la eficiencia de las plantas desalinizadoras de agua de mar utilizando sistemas de control inteligente, conformados por controladores multivariables fundamentados en redes neuronales artificiales y modelos matemáticos multivariables basados en las leyes de conservación de la materia y energía. Se empieza describiendo las tecnologías empleadas a nivel industrial para la desalinización de agua, y se continúa con un...

  4. Aplicación de redes neuronales artificiales y técnicas sig para la predicción de coberturas forestales

    OpenAIRE

    Á. Leyva Ovalle; S. Terrazas Domínguez; E. Buendía Rodríguez; E. Vargas Pérez

    2002-01-01

    En México, como en todo el mundo, se tiene la necesidad de realizar un inventario de sus recursos forestales, a fin de llevar a cabo en forma oportuna y adecuada la planeación y la ejecución de programas de manejo forestal. Un método para la obtención de este tipo de información es a través de modelos de predicción. El presente estudio tiene como objetivo evaluar la habilidad de las redes neuronales artificiales (RNA) en la predicción de tipos de coberturas forestales. Las RNA desarrolladas e...

  5. Estudios del rol modulador de ghrelina sobre la actividad neuroendocrina: circuitos neuronales implicados en la activación de las neuronas CRF hipofisiotrópicas

    OpenAIRE

    2014-01-01

    En la presente Tesis Doctoral estudiamos los circuitos neuronales que median los efectos de la ghrelina sobre las neuronas CRF hipofisiotrópicas del NPV y el eje HHA. Esperamos que el esclarecimiento de estos circuitos pueda ayudar a entender cómo funcionan y cómo se integran la homeostasis energética y el estrés. Así como también, contribuir al desarrollo de futuras terapias que intenten manipular las acciones de la ghrelina. Facultad de Ciencias Exactas

  6. Aplicación de las redes neuronales pulsantes en el reconocimiento de patrones y análisis de imágenes

    OpenAIRE

    Matadamas Ortiz, Idarh Claudio

    2014-01-01

    Resumen En la presente tesis se propone un método para la aplicación de Redes Neuronales Pulsantes a problemas de reconocimiento de patrones y análisis de imágenes, la arquitectura empleada para la red neuronal hace uso del modelo neuronal de Izhikevich como unidad de procesamiento; el entrenamiento no supervisado y ajuste de los parámetros libres, se realizan por medio de un algoritmo de Evolución Diferencial programado en lenguaje C++. Usando una arquitectura feedforward con tres ca...

  7. Avances en el diagnóstico de fallas en sistemas eléctricos de transporte mediante redes neuronales: Un enfoque modular

    Directory of Open Access Journals (Sweden)

    Francisco Morant Anglada

    2011-09-01

    Full Text Available Normal 0 21 false false false MicrosoftInternetExplorer4 /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Tabla normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-parent:""; mso-padding-alt:0cm 5.4pt 0cm 5.4pt; mso-para-margin:0cm; mso-para-margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:10.0pt; font-family:"Times New Roman"; mso-ansi-language:#0400; mso-fareast-language:#0400; mso-bidi-language:#0400;} En este trabajo se propone un nuevo método para el diagnóstico de fallas en sistemas eléctricos de potencia basado en módulos neuronales. El método realiza el diagnóstico mediante la asignación de un módulo neuronal genérico para cada tipo de componente que conforma al sistema eléctrico de potencia, ya sea línea de transporte, bus o transformador. En total se diseñan tres módulos neuronales genéricos, uno para cada tipo de componente. Los módulos neuronales para buses y transformadores se componen de dos niveles de diagnóstico tomando en cuenta los estados lógicos de interruptores y relevadores tanto propios como de respaldo, a excepción del módulo neuronal para líneas de transporte, que además de los dos niveles de diagnóstico con los que cuentan los módulos neuronales para buses y transformadores, cuenta con un tercer nivel de diagnóstico que toma en consideración los oscilogramas de voltajes y corrientes de falla, así como los espectros de frecuencia  de estos oscilogramas, para verificar si la línea de transporte realmente estuvo sujeta a una falla y a la vez determinar el tipo de ésta (L-g, LL-g, LL, LLL, LLL-g, esto a través de una estructura neuronal. Este tercer nivel de diagnóstico, es posible llevarlo a cabo ya que toda línea de transporte  que es sometida a una falla presentará corrientes y voltajes de falla, antes de que ésta sea liberada del sistema  por la acción de sus esquemas de protección.

  8. Aplicación de las redes neuronales artificiales a la predicción del resultado a corto, medio y largo plazo del transplante renal pediatrico.

    OpenAIRE

    Serrano Durbá, Agustin

    2004-01-01

    RESUMEN Introducción: Se plantea la aplicación de esta herramienta novedosa en el problema de la indicación del trasplante renal infantil (TRI). Objetivos: 1) Desarrollar un modelo neuronal para identificar, con la mayor sensibilidad y especificidad, la evolución a corto, medio y largo plazo del TRI mediante el empleo de factores pre-trasplante. 2) Contrastar la capacidad clasificatoria de las redes neuronales artificiales (RNA) frente a la regresión logística (RL) en el TRI. 3) Determi...

  9. Tendencias en la predicción y estimación de los intervalos de confianza usando modelos de redes neuronales aplicados a series temporales

    Directory of Open Access Journals (Sweden)

    JUAN DAVID VELÁSQUEZ H.

    2006-01-01

    Full Text Available En este artículo, se discute el estado del arte en la estimación de la predicción varios pasos adelante para modelos de series temporales no lineales basados en perceptrones multicapa. Se revisan las principales tendencias para la estimación de pronósticos puntuales e intervalos de confianza. En adición, se argumenta sobre los principales problemas abiertos para investigación futura en la predicción de series temporales usando redes neuronales.

  10. Loin des sciences sociales.

    Directory of Open Access Journals (Sweden)

    René-Éric Dagorn

    2002-05-01

    Full Text Available Plusieurs numéros de Livres Hebdo et du Monde des Livres des mois de janvier-février 2002 permettent de proposer un rapide survol des grandes ventes de l’année 2001 et de quelques tendances éditoriales de l’année 2002. Quelques points ressortent particulièrement de la lecture des articles de ces deux sources : l’intérêt de la nouvelle méthode d’estimation des ventes proposée par l’institut Ipsos et Livres Hebdo , le tableau des grandes ventes en 2001 (et ...

  11. La metamorphose des cypris femelles des Rhizocephales

    NARCIS (Netherlands)

    Veillet, A.

    1964-01-01

    Depuis la découverte de la métamorphose des cypris de Sacculina carcini Thompson par Delage, peu de biologistes se sont intéressés au développement des Rhizocéphales. On admet aujourd'hui que tous les Cirripèdes parasites ont, comme Sacculina carcini, une forme kentrogone qui inocule le parasite au

  12. Detección en línea de onda-P en sismogramas 3-D utilizando redes neuronales

    Directory of Open Access Journals (Sweden)

    Alzate Carlos

    2003-08-01

    Full Text Available

    We have developed a P-wave arrival time detector of tectonic events over digital three-component records online. This process uses two different methods: the first one works with a LVR detector, and the second one uses a statistical classifier of classes (signal with P-wave and signal with seismic noise. Detection uses multi-layer perceptron neural network where is taken advantage of non-lineal input-output, relation, well-generalization, low computational demands, etc. These appropriate qualities for real time signal processing. The input data for the system becomes a normalized vector of seismic signal and its polarization arguments. Testes were done with data from IRIS and INGEOMINAS database. Although the system has a good perforance, its accuracy is not acceptable.

    Se desarrolla un detector en línea del tiempo de arribo de ondas P sobre registros electrónicos de tres componentes de eventos tectónicos. Se lleva a cabo la detección en línea de la onda-p empleando redes neuronales (RN, utilizando dos diferentes formas: en la primera se sintetiza el detector de LRV, mientras en la segunda se desarrolla un clasificador estadístico con las respectivas clases (señal con onda-p presente y señal con solo ruido presente. La detección se realiza empleando redes neuronales de tipo perceptrón multi-capa, en las cuales se aprovechan las siguientes cualidades: su capacidad de mapeo no lineal de entrada-salida, buena generalización, bajo costo computacional, entre otras. Las anteriores cualidades hacen que las RN sean apropiadas para el proceso de señales en tiempo real. Las entradas a la RN corresponden a

  13. PRONÓSTICO DE VENTAS: COMPARACIÓN DE PREDICCIÓN ENTRE REDES NEURONALES Y MÉTODO ESTADÍSTICO [doi: 10.5329/RECADM.20030201008

    Directory of Open Access Journals (Sweden)

    S. Nojek

    2003-05-01

    Full Text Available Normal 0 21 false false false PT-BR X-NONE X-NONE MicrosoftInternetExplorer4 RESUMEN Actualmente es común que las empresas realicen pronósticos de ventas para poder planificar más acertadamente su producción. El objetivo de este trabajo consiste en estudiar la predicción de ventas mediante el uso de redes neuronales, comparar los resultados obtenidos con los pronósticos de un método estadístico clásico y establecer los entornos más adecuados para su uso. Palabras Clave: Pronostico de Ventas – Predicción basada en Redes Neuronales.   ABSTRACT Companies usually need sales forecast for improving production planning. This research work pursues to study sales forecast based on neural networks and to compare this results with the ones based on classical statistical methods trying to specify the adequate business environment for using them Keywords: Sales Forecast – Forecasting based on Neural Networks

  14. FSH isoform pattern in classic galactosemia

    OpenAIRE

    Gubbels, C.S.; Thomas, C. M.; Wodzig, K.W.H.; Olthaar, A. J.; Jaeken, J.; Sweep, F.C.; Rubio-Gozalbo, M.E.

    2010-01-01

    Female classic galactosemia patients suffer from primary ovarian insufficiency (POI). The cause for this long-term complication is not fully understood. One of the proposed mechanisms is that hypoglycosylation of complex molecules, a known secondary phenomenon of galactosemia, leads to FSH dysfunction. An earlier study showed less acidic isoforms of FSH in serum samples of two classic galactosemia patients compared to controls, indicating hypoglycosylation. In this study, FSH isoform patterns...

  15. Sample displacement chromatography of plasmid DNA isoforms.

    Science.gov (United States)

    Černigoj, Urh; Martinuč, Urška; Cardoso, Sara; Sekirnik, Rok; Krajnc, Nika Lendero; Štrancar, Aleš

    2015-10-02

    Sample displacement chromatography (SDC) is a chromatographic technique that utilises different relative binding affinities of components in a sample mixture and has been widely studied in the context of peptide and protein purification. Here, we report a use of SDC to separate plasmid DNA (pDNA) isoforms under overloading conditions, where supercoiled (sc) isoform acts as a displacer of open circular (oc) or linear isoform. Since displacement is more efficient when mass transfer between stationary and mobile chromatographic phases is not limited by diffusion, we investigated convective interaction media (CIM) monoliths as stationary phases for pDNA isoform separation. CIM monoliths with different hydrophobicities and thus different binding affinities for pDNA (CIM C4 HLD, CIM-histamine and CIM-pyridine) were tested under hydrophobic interaction chromatography (HIC) conditions. SD efficiency for pDNA isoform separation was shown to be dependent on column selectivity for individual isoform, column efficiency and on ammonium sulfate (AS) concentration in loading buffer (binding strength). SD and negative mode elution often operate in parallel, therefore negative mode elution additionally influences the efficiency of the overall purification process. Optimisation of chromatographic conditions achieved 98% sc pDNA homogeneity and a dynamic binding capacity of over 1mg/mL at a relatively low concentration of AS. SDC was successfully implemented for the enrichment of sc pDNA for plasmid vectors of different sizes, and for separation of linear and and sc isoforms, independently of oc:sc isoform ratio, and flow-rate used. This study therefore identifies SDC as a promising new approach to large-scale pDNA purification, which is compatible with continuous, multicolumn chromatography systems, and could therefore be used to increase productivity of pDNA production in the future. Copyright © 2015 Elsevier B.V. All rights reserved.

  16. ANALYSE DES MODES DE GESTION DES ENTREPRISES ...

    African Journals Online (AJOL)

    Administrateur

    L'étude de l'art et des biens culturels en général est longtemps restée en ... en économie et management), Université d'Angers / Chef de projet chez Arterial Network. ... culturelles publiques sont apparues à partir de la fin des années 1990.

  17. Des racines et des ailes

    Directory of Open Access Journals (Sweden)

    Stéphanie Vincent-Geslin

    2012-05-01

    Full Text Available Les mobilités pendulaires semblent être en augmentation en Europe depuis une dizaine d’années. Cette croissance du temps passé à se déplacer amène à remettre en question la conjecture de Zahavi et apparaît relativement inexplicable en regard du paradigme classique de l’acteur rationnel traditionnellement utilisé dans le champ des transports. Si, dans la littérature, les temps de déplacements sont principalement expliqués par le contexte résidentiel, la forme urbaine et le travail, ce cadre explicatif ne dit rien des processus de décision eux-mêmes qui amènent aux pendularités intensives.À partir d’une enquête qualitative menée auprès de pendulaires français, suisses et belges, cette contribution propose d’analyser les arbitrages et les éléments déterminants des processus de la grande pendularité. Les mobilités quotidiennes pendulaires apparaissent comme le résultat de compromis entre activité professionnelle, attachement résidentiel et choix de vie et prennent ainsi la forme de stratégies de conciliation entre vie privée et vie professionnelle. Ces mobilités spatiales permettent alors paradoxalement la préservation des ancrages résidentiels, sociaux et familiaux.Roots and wings. Long-distance commuting patterns, or how to conciliate professional and personal lifeLong-distance commuting patterns appear to be increasing in Europe over the last ten years. These raising mobility patterns lead to reappraise the Zahavi conjecture and appear largely inexplicable by the classical rational actor paradigm traditionally used in transportation research. In literature, commuting is mainly explained by residential contexts, urban forms and job. Nevertheless this theoretical frame says little about the decision-making processes themselves. Based on a qualitative survey conducted in three European countries - France, Belgium and Switzerland – among a population of high commuters, this paper proposes an analysis of

  18. Artificial Neural Networks for Predicting Real Estate Prices || Redes neuronales artificiales para la predicción de precios inmobiliarios

    Directory of Open Access Journals (Sweden)

    Núñez Tabales, Julia M.

    2013-01-01

    Full Text Available Econometric models, in the estimation of real estate prices, are a useful and realistic approach for buyers and for local and fiscal authorities. From the classical hedonic models to more data driven procedures, based on Artificial Neural Networks (ANN, many papers have appeared in economic literature trying to compare the results attained with both approaches. We insist on the use of ANN, when there is enough statistical information, and will detail some comparisons to hedonic modeling, in a medium size city in the South of Spain, with an extensive set of data spanning over several years, collected before the actual downturn of the market. Exogenous variables include each dwelling's external and internal data (both numerical and qualitative, and data from the building in which it is located and its surroundings. Alternative models are estimated for several time intervals, and enabling the comparison of the effects of the rising prices during the bull market over the last decade. || Los modelos econométricos en la valoración de precios inmobiliarios constituyen una herramienta útil tanto para los compradores como para las autoridades locales y fiscales. Desde los modelos hedónicos clásicos hasta los planteamientos actuales a través de redes neuronales artificiales (RNA, han tenido lugar numerosas aportaciones en la literatura económica que tratan de comparar los resultados de ambos métodos. Insistimos en el empleo de RNA en el caso de disponer de suficiente información estadística. En este trabajo se aplica dicha metodología en una ciudad de tamaño medio situada en el sur de España, utilizando una extensa muestra de datos que comprende varios años precedentes a la crisis actual. Las variables utilizadas -tanto cuantitativas como cualitativas- incluyen datos externos e internos de la vivienda, del edificio en el que está localizada, así como de su entorno. Se construyen varios modelos alternativos para distintos intervalos de

  19. Neuronal networks for the study of buildings thermal behavior; Redes neuronales para el estudio del comportamiento termico de edificios

    Energy Technology Data Exchange (ETDEWEB)

    Marincic, Irene [Universidad de Sonora (Mexico); Del Rio, J. Antonio [Centro Morelense de Innovacion y Transferencia Tecnologica (Mexico)

    2009-07-15

    The evaluation as well as the prediction of buildings thermal behavior involves complex calculations mainly because of its enormous number of variables, many of them difficult to determine. For this type of problems, a possibility is to resort to models of the black box type, that allow the characterization of the building with very few variables, for then to be able to predict its behavior under other circumstances, with greater certainty. One of the black box models of more and more used in diverse disciplines, is the one of the artificial neuronal networks (ANN). These can be used as an alternative method of analysis and prediction, particularly for cases in which many variables take part. In this work a preliminary study is presented that analyzes the accuracy of a model of neuronal network for the prediction of the inside temperatures of a building, given the outside temperatures. A very simple network, of 3 neurons, located in 2 layers is considered. The results of the inside temperatures obtained in the learning as well as in the prediction stage are analyzed, comparing them with the ones obtained using another model of black box previously developed by the authors, the one of the function responded in the dominion of frequencies. [Spanish] Tanto la evaluacion como la prediccion del comportamiento termico de los edificios involucra calculos complejos sobre todo por su enorme numero de variables, muchas de ellas dificiles de determinar. Para este tipo de problemas, una posibilidad es recurrir a modelos del tipo caja negra, que permiten caracterizar al edificio como muy pocas variables, para luego poder predecir su comportamiento bajo otras circunstancias, como mayor certeza. Uno de los modelos de caja negra cada vez mas utilizado en diversas disciplinas, es el de las redes neuronales artificiales (ANN). Estas pueden ser usadas como metodo alternativo de analisis y sobre todo de prediccion, particularmente para los casos en que intervienen muchas variables. En

  20. Antiangiogenic VEGF Isoform in Inflammatory Myopathies

    Directory of Open Access Journals (Sweden)

    Nila Volpi

    2013-01-01

    Full Text Available Objective. To investigate expression of vascular endothelial growth factor (VEGF antiangiogenic isoform A-165b on human muscle in idiopathic inflammatory myopathies (IIM and to compare distribution of angiogenic/antiangiogenic VEGFs, as isoforms shifts are described in other autoimmune disorders. Subjects and Methods. We analyzed VEGF-A165b and VEGF-A by western blot and immunohistochemistry on skeletal muscle biopsies from 21 patients affected with IIM (polymyositis, dermatomyositis, and inclusion body myositis and 6 control muscle samples. TGF-β, a prominent VEGF inductor, was analogously evaluated. Intergroup differences of western blot bands density were statistically examined. Endomysial vascularization, inflammatory score, and muscle regeneration, as pathological parameters of IIM, were quantitatively determined and their levels were confronted with VEGF expression. Results. VEGF-A165b was significantly upregulated in IIM, as well as TGF-β. VEGF-A was diffusely expressed on unaffected myofibers, whereas regenerating/atrophic myofibres strongly reacted for both VEGF-A isoforms. Most inflammatory cells and endomysial vessels expressed both isoforms. VEGF-A165b levels were in positive correlation to inflammatory score, endomysial vascularization, and TGF-β. Conclusions. Our findings indicate skeletal muscle expression of antiangiogenic VEGF-A165b and preferential upregulation in IIM, suggesting that modulation of VEGF-A isoforms may occur in myositides.

  1. Ikaros isoforms:The saga continues

    Institute of Scientific and Technical Information of China (English)

    Laura; A; Perez-Casellas; Aleksandar; Savic; Sinisa; Dovat

    2011-01-01

    Through alternate splicing,the Ikaros gene produces multiple proteins.Ikaros is essential for normal hematopoiesis and possesses tumor suppressor activity.Ikaros isoforms interact to form dimers and potentially multimeric complexes.Diverse Ikaros complexes produced by the presence of different Ikaros isoforms are hypothesized to confer distinct functions.Small dominantnegative Ikaros isoforms have been shown to inhibit the tumor suppressor activity of full-length Ikaros.Here,we describe how Ikaros activity is regulated by the coordinated expression of the largest Ikaros isoforms IK-1 and IK-H.Although IK-1 is described as full-length Ikaros,IK-H is the longest Ikaros isoform.IK-H,which includes residues coded by exon 3B (60 bp that lie between exons 3 and 4),is abundant in human but not murine hematopoietic cells.Specific residues that lie within the 20 amino acids encoded by exon 3B give IK-H DNA-binding characteristics that are distinct from those of IK-1.Moreover,IK-H can potentiate or inhibit the ability of IK-1 to bind DNA.IK-H binds to the regulatory regions of genes that are upregulated by Ikaros,but not genes that are repressed by Ikaros.Although IK-1 localizes to pericentromeric heterochromatin,IK-H can be found in both pericentromeric and non-pericentromeric locations.Anti-silencing activity of gamma satellite DNA has been shown to depend on the binding of IK-H,but not other Ikaros isoforms.The unique features of IK-H,its influence on Ikaros activity,and the lack of IK-H expression in mice suggest that Ikaros function in humans may be more complex and possibly distinct from that in mice.

  2. Optimización de secciones de vigas y columnas para el cumplimiento de la deriva en edificaciones de concreto reforzado mediante Redes Neuronales Artificiales

    Directory of Open Access Journals (Sweden)

    Jorge Arcila Zea

    2014-01-01

    Full Text Available Este artículo presenta los resultados del uso de Redes Neuronales Artificiales (RNA para la estimación de secciones óptimas de vigas y columnas en concreto reforzado en edificaciones aporticadas simétricas de 1 a 6 pisos teniendo en cuenta los requisitos mínimos exigidos en la NSR-10 relacionados con la deriva y el diseño sísmico. Además se estudió la sensibilidad de la deriva respecto a los valores de dimensiones de vigas y de columnas, para que una vez se tenga una mejor comprensión de dicha relación, se puedan obtener diseños óptimos de manera más rápida, sencilla y confiable en comparación con los procedimientos utilizados actualmente.

  3. APROXIMACIÓN NO LINEAL AL MODELO DE OVERSHOOTING USANDO REDES NEURONALES MULTlCAPA PARA EL TIPO DE CAMBIO DÓLAR-PESO

    Directory of Open Access Journals (Sweden)

    Jaime Villamil

    2009-06-01

    Full Text Available Desde los años setenta muchos trabajos han intentado elaborar una sustentación empírica de algunos modelos que ofrecieron una explicación lineal de la dinámica de la tasa de cambio de un país, entre ellos el de Dornbusch. Hasta el momento ninguno ha sido concluyente y la caminata aleatoria es considerada como el mejor modelo al que puede ajustarse. De Grauwe ha mostrado que, con la presencia de relaciones no-lineales y heterogeneidad de expectativas de los especuladores, el tipo de cambio puede tener un comportamiento aparentemente aleatorio, pero con explicación determinista. Este trabajo presenta el modelo de Dornbusch en la versión no lineal propuesta por De Grauwe y Dewachter (1993, y una aproximación usando redes neuronales multicapa aplicadas al caso del dólar/peso (USD/COP.

  4. Aproximación no lineal al modelo de overshooting usando redes neuronales multicapa para el tipo de cambio dólar - peso

    Directory of Open Access Journals (Sweden)

    Villamil Jaime

    2009-10-01

    Full Text Available Desde los años setenta muchos trabajos han intentado elaborar una sustentación empírica de algunos modelos
    que ofrecieron una explicación lineal de la dinámica de la tasa de cambio de un país, entre ellos el de Dornbusch. Hasta el momento ninguno ha sido concluyente y la caminata aleatoria es considerada como el mejor modelo al que puede ajustarse. De Grauwe ha mostrado que, con la presencia de relaciones no-lineales y heterogeneidad de expectativas de los especuladores, el tipo de cambio puede tener un comportamiento aparentemente aleatorio, pero con explicación determinista. Este trabajo presenta el modelo de Dornbusch en la versión no lineal propuesta por De Grauwe y Dewachter (1993, y una aproximación usando redes neuronales multicapa aplicadas al caso del dólar/peso (USD/COP.

  5. Teoría de precios de arbitraje. Evidencia empírica para Colombia a través de redes neuronales

    Directory of Open Access Journals (Sweden)

    Charle Londoño

    2011-07-01

    Full Text Available Esta investigación utiliza una red neuronal multicapa para relacionar el Índice General de Bolsa de Valores de Colombia (IGBC con fundamentales macroeconómicos y variables financieras. Proponemos dos modelos: un modelo APT (fundamentales macroeconómicos y un modelo APT modificado (fundamentales macroeconómicos + indicador de las bolsas del mundo; de acuerdo a nuestro análisis el APT tradicional se ajusta mejor para predecir el mercado de valores Colombiano. Los resultados confirman que las redes neuronales artificiales (ANN son más efectivas que los modelos estadísticos tradicionales por su capacidad explicativa y precisión.

  6. Modelación del crecimiento de pollitas Lohmann LSL con redes neuronales y modelos de regresión no lineal

    Directory of Open Access Journals (Sweden)

    Luis Galeano-Vasco

    2013-11-01

    Full Text Available Objetivo. Modelar la curva del crecimiento de aves de la línea Lohmann LSL utilizando modelos no lineales (MNL, no lineales mixtos (MNLM y redes neuronales artificiales (RNA. Materiales y métodos. Periódicamente se pesaron 33 aves en promedio, desde el día 21 al 196 de vida para un total de 558 registros individuales de peso. En el ajuste de la curva de crecimiento se utilizaron los modelos: no lineal de Von Bertalanffy (MNL, no lineal Mixto de Von Bertalanffy (MNLM y redes neuronales artificiales (RNA. Los modelos se compararon con coeficiente de correlación y medidas de presicion cuadrado medio del error (CME, desviación media absoluta (MAD y porcentaje de la media absoluta del error (MAPE. Resultados. Los valores de correlación entre los datos reales y estimados, fueron 0.999, 0.990 y 0.986 para MNLM, RNA y MNL respectivamente. El modelo más preciso con base en los criterios MAPE, MAD y CME fue el MNLM, seguido por la RNA. La grafica de predicción generada la RNA es similar a la del MNLM. La RNA presentó un desempeño superior al MLN. Conclusiones. El mejor modelo para la predicción de curvas de crecimiento de aves comerciales de la línea Lohmman LSL hasta los 196 días de edad, con múltiples mediciones por animal en el tiempo, fue el MNLM. La RNA presento un desempeño superior al MNL.

  7. Pronóstico del rendimiento del IPC (Índice de Precios y Cotizaciones mediante el uso de redes neuronales diferenciales

    Directory of Open Access Journals (Sweden)

    Agustín Ignacio Cabrera Llanos

    2012-01-01

    Full Text Available A través de los años, el uso de las redes neuronales artificiales como herramienta de simulación, modelación y descripción de sistemas dinámicos no lineales se ha ido consolidando como una técnica eficaz y relativamente rápida gracias al gran desarrollo experimentado en los sistemas de cómputo. Esta técnica de uso común en algunas áreas de la ingeniería aplicada se empezó a utilizar en aplicaciones financieras a mediados de la década de los noventa. El presente trabajo utiliza una de las técnicas más recientes y poderosas en este ámbito: las redes neuronales diferenciales (RND, usada frecuentemente en análisis de procesos de la biotecnología. Mediante esta técnica se lleva a cabo el análisis y la estimación de la evolución del comportamiento del rendimiento del IPC de la Bolsa Mexicana de Valores (BMV durante el periodo comprendido del 8 de noviembre de 1991 al 27 de enero de 2011. Adicionalmente, el análisis incluye un pronóstico intradía (seis valores durante una jornada de operación de la BMV del rendimiento del IPC, el cual tiene una duración de un día después del último dato de la serie de tiempo del IPC. Cabe señalar que los resultados pronosticados mostraron una gran similitud con los datos reales.

  8. Liste des intrants 2015

    OpenAIRE

    Speiser, Bernhard; Tamm, Lucius; Maurer, Veronika; Berner, Alfred; Schneider, Claudia; Chevillat, Véronique

    2015-01-01

    La liste des intrants contient tous les produits phytosanitaires, les engrais, les substrats du commerce, les produits de lutte contre les mouches des étables, les agents d'ensilage, les aliments minéraux et complémentaires, les produits pour la désinfection des stabulations et les produits contre les maladies des abeilles autorisés pour l'agriculture biologique. Cette liste est contraignante pour les producteurs de Bio Suisse. Sur les fermes Bio Suisse, seuls les produits mentionnés sont aut...

  9. New isoforms of rat Aquaporin-4

    DEFF Research Database (Denmark)

    Moe, Svein Erik; Sorbo, Jan Gunnar; Søgaard, Rikke;

    2008-01-01

    an intracellular localization when expressed in cell lines and do not transport water when expressed in Xenopus oocytes. In contrast, the largest of the new isoforms, AQP4e, which contains a novel N-terminal domain, is localized at the plasma membrane in cell lines and functions as a water transporter in Xenopus...

  10. p53 isoforms change p53 paradigm

    OpenAIRE

    2014-01-01

    Although p53 defines cellular responses to cancer treatment it is not clear how p53 can be used to control cell fate outcome. Data demonstrate that so-called p53 does not exist as a single protein, but is in fact a group of p53 protein isoforms whose expression can be manipulated to control the cellular response to treatment.

  11. Isoforms of receptors of fibroblast growth factors.

    Science.gov (United States)

    Gong, Siew-Ging

    2014-12-01

    The breadth and scope of Fibroblast Growth Factor signaling is immense, with documentation of its role in almost every organism and system studied so far. FGF ligands signal through a family of four distinct tyrosine kinase receptors, the FGF receptors (FGFRs). One contribution to the diversity of function and signaling of FGFs and their receptors arises from the numerous alternative splicing variants that have been documented in the FGFR literature. The present review discusses the types and roles of alternatively spliced variants of the FGFR family members and the significant impact of alternative splicing on the physiological functions of five broad classes of FGFR isoforms. Some characterized known regulatory mechanisms of alternative splicing and future directions in studies of FGFR alternative splicing are also discussed. Presence, absence, and/or the combination of specific exons within each FGFR protein impart upon each individual isoform its unique function and expression pattern during normal function and in diseased states (e.g., in cancers and birth defects). A better understanding of the diversity of FGF signaling in different developmental contexts and diseased states can be achieved through increased knowledge of the presence of specific FGFR isoforms and their impact on downstream signaling and functions. Modern high-throughput techniques afford an opportunity to explore the distribution and function of isoforms of FGFR during development and in diseases.

  12. Absolute quantitation of protein posttranslational modification isoform.

    Science.gov (United States)

    Yang, Zhu; Li, Ning

    2015-01-01

    Mass spectrometry has been widely applied in characterization and quantification of proteins from complex biological samples. Because the numbers of absolute amounts of proteins are needed in construction of mathematical models for molecular systems of various biological phenotypes and phenomena, a number of quantitative proteomic methods have been adopted to measure absolute quantities of proteins using mass spectrometry. The liquid chromatography-tandem mass spectrometry (LC-MS/MS) coupled with internal peptide standards, i.e., the stable isotope-coded peptide dilution series, which was originated from the field of analytical chemistry, becomes a widely applied method in absolute quantitative proteomics research. This approach provides more and more absolute protein quantitation results of high confidence. As quantitative study of posttranslational modification (PTM) that modulates the biological activity of proteins is crucial for biological science and each isoform may contribute a unique biological function, degradation, and/or subcellular location, the absolute quantitation of protein PTM isoforms has become more relevant to its biological significance. In order to obtain the absolute cellular amount of a PTM isoform of a protein accurately, impacts of protein fractionation, protein enrichment, and proteolytic digestion yield should be taken into consideration and those effects before differentially stable isotope-coded PTM peptide standards are spiked into sample peptides have to be corrected. Assisted with stable isotope-labeled peptide standards, the absolute quantitation of isoforms of posttranslationally modified protein (AQUIP) method takes all these factors into account and determines the absolute amount of a protein PTM isoform from the absolute amount of the protein of interest and the PTM occupancy at the site of the protein. The absolute amount of the protein of interest is inferred by quantifying both the absolute amounts of a few PTM

  13. Apolipoprotein (A) Isoform Distribution and Plasma Lipoprotein (a ...

    African Journals Online (AJOL)

    Apolipoprotein (A) Isoform Distribution and Plasma Lipoprotein (a) Levels In Nigerian Subjects With and Without Coronary Heart Disease. ... Plasma lipoprotein (a) Concentrations and apo(a) isoforms were determined in 101 ... Article Metrics.

  14. Contribution à l'étude de la diversité biochimique et biologique des agents des encéphalopathies spongiformes transmissibles

    OpenAIRE

    2012-01-01

    Les encéphalopathies spongiformes transmissibles (EST) sont des maladies neurodégénératives affectant de nombreuses espèces de mammifères dont l’Homme (maladie de Creutzfeldt-Jakob – MCJ), la vache (encéphalopathie spongiforme bovine – ESB) et le mouton (tremblante). Au cours de ces maladies, une protéine, PrPSc, isoforme anormale de la protéine cellulaire PrP C, s’accumule dans le système nerveux central des individus atteints. Selon le concept du prion, la PrPSc elle-même serait l’agent ...

  15. Gestion des risques

    CERN Document Server

    Louisot, Jean-Paul

    2009-01-01

    Depuis le début du lie siècle, la gestion des risques connaît une véritable révolution culturelle. Jusqu'alors fonction technique, centrée autour de l'achat de couverture d'assurances, elle est devenue une discipline managériale et transversale : une valise d'instruments que chaque manager doit connaître et appliquer quels que soient son domaine de compétence et ses missions au sein de l'organisation. En effet, la gestion des risques est une culture qui doit être assimilée par chacun des acteurs. C'est précisément l'ambition des 101 questions rassemblées dans cet ouvrage : apporter à chaque manager d'entreprise, de collectivité, d'établissement de santé..., des réponses claires au " pourquoi " et au " comment " : Comment identifier les risques ? Comment analyser les risques ? Quels sont les objectifs de la gestion des risques ? Une carte des risques pour quoi faire ? Pourquoi faut-il financer les risques ? Les entreprises ont-elles des responsabilités pénales ? En quoi consiste la gestion...

  16. Le dialogisme intertextuel des contes des Grimm

    OpenAIRE

    2012-01-01

    « Le caractère le plus important de l’énoncé, ou en tous cas le plus ignoré, est son dialogisme, c’est-à-dire sa dimension intertextuelle », constate Todorov en référence à la conception dialogique du langage proposée par Bakthine. Cet article introductif postule que ce constat s’applique aussi aux contes des Grimm. En partant des recherches déjà menées sur Apulée, Straporola, Basile, Perrault, La Fontaine et Lhéritier, il présente des concepts (réponse intertextuelle, reconfiguration génériq...

  17. Isoforms of murine and human serum amyloid P component

    DEFF Research Database (Denmark)

    Nybo, Mads; Hackler, R; Kold, B

    1998-01-01

    Isoelectric focusing (IEF) and immunofixation of murine serum amyloid P component (SAP), purified and in serum, showed a distinct and strain-dependent isoform pattern with up to seven bands (pI 5.1-5.7). Neuraminidase treatment caused a shift of the isoforms to more basic pI values, but did...... of isoforms of human SAP required the presence of urea and higher SAP concentrations. TEF and immunofixation of SAP monomers showed five to eight isoforms, ranging from pI 4.7-5.7. IEF of SAP in human serum resulted in a less distinct pattern and more acidic isoforms. As with murine SAP, neuraminidase...

  18. Subpoblaciones neuronales presentes en cultivos primarios de ganglio espinal y su relación con la infección in vitro por virus de rabia

    Directory of Open Access Journals (Sweden)

    Hernán Hurtado

    2000-02-01

    Full Text Available

    El virus de la rabia presenta un fuerte tropismo neuronal y que produce una encefalitis generalmente letal. En un accidente rábico clásico, producido por la mordedura de un animal infectado, el virus es captado por terminaciones nerviosas motoras, autonómicas o sensoriales. Si es captado por estas últimas, el virus se transporta retrógradamente hacia los somas neuronales ubicados en los ganglios espinales y de ahí pasa hasta el Sistema Nervioso Central en donde ejerce su acción patógena. Por este motivo los cultivos de ganglio espinal son un modelo relevante para estudiar la interacción entre el virus de la rabia y las neuronas sensoriales presentes en ellos. En estos cultivos se encuentran dos tipos de células, neuronas y células no neuronales (fibroblastos y células de Schwann. Al inocular los cultivos con virus de rabia cepa CVS (Challenge Virus Standard, a pesar de que las células no neuronales son la mayoría, las que se infectan en mayor proporción son las neuronas, lo que confirma el marcado neurotropismo del virus. Adicionalmente a esto, dentro de la población neuronal, parece existir una subpoblación con mayor susceptibilidad hacia la infección por el virus. Para comprobar esta hipótesis, se caracterizaron las subpoblaciones neuronales presentes en estos cultivos utilizando un criterio morfológico (diámetro neuronal y uno bioquímico (presencia de neuropéptidos como marcadores de subpoblaciones en el ganglio. Se realizó una técnica de doble inmunocitoquímica para virus rábico y los neuropéptidos Sustancia P (SP, Neuropéptido Y (NPY, Galanina (GAL, Péptido Relacionado con el Gen de la Calcitonina (CGRP y Péptido Intestinal Vasoactivo (VIP, que clásicamente se usan para la definición de subpoblaciones. El análisis morfométrico demostró que en nuestros cultivos el 85% de las neuronas presentes son de pequeño diámetro (<25

  19. Functional studies of sodium pump isoforms

    DEFF Research Database (Denmark)

    Clausen, Michael Jakob

    The Na+,K+-ATPase is an essential ion pump found in all animal cells. It uses the energy from ATP hydrolysis to export three Na+ and import two K+, both against their chemical gradients and for Na+ also against the electrical potential. Mammals require four Na+,K+-ATPase isoforms that each have u...... synthesized cohorts of pumps from the Golgi apparatus to the plasma membrane....

  20. FSH isoform pattern in classic galactosemia.

    Science.gov (United States)

    Gubbels, Cynthia S; Thomas, Chris M G; Wodzig, Will K W H; Olthaar, André J; Jaeken, Jaak; Sweep, Fred C G J; Rubio-Gozalbo, M Estela

    2011-04-01

    Female classic galactosemia patients suffer from primary ovarian insufficiency (POI). The cause for this long-term complication is not fully understood. One of the proposed mechanisms is that hypoglycosylation of complex molecules, a known secondary phenomenon of galactosemia, leads to FSH dysfunction. An earlier study showed less acidic isoforms of FSH in serum samples of two classic galactosemia patients compared to controls, indicating hypoglycosylation. In this study, FSH isoform patterns of five classic galactosemia patients with POI were compared to the pattern obtained in two patients with a primary glycosylation disorder (phosphomannomutase-2-deficient congenital disorders of glycosylation, PMM2-CDG) and POI, and in five postmenopausal women as controls. We used FPLC chromatofocussing with measurement of FSH concentration per fraction, and discovered that there were no significant differences between galactosemia patients, PMM2-CDG patients and postmenopausal controls. Our results do not support that FSH dysfunction due to a less acidic isoform pattern because of hypoglycosylation is a key mechanism of POI in this disease.

  1. Photonique des Morphos

    CERN Document Server

    Berthier, Serge

    2010-01-01

    La photonique est déjà présente dans notre vie quotidienne, et on attend maintenant que la manipulation des photons permette aussi le traitement logique des informations. Cependant, l’élément de base qui permet cette manipulation de la lumière, le cristal photonique, est d’une réalisation complexe et mal contrôlée. Dans la course à la maîtrise de la lumière, les structures photoniques naturelles ont beaucoup à nous apprendre. C’est ce que nous montre Serge Berthier qui étudie dans ce livre la structure des écailles des Morphos. Tenant compte de l’essor récent des approches biomimétiques, il présente de manière détaillée plus de dix-huit techniques expérimentales utilisées pour ses analyses, ainsi que les diverses approches théoriques développées pour la modélisation de structures multi-échelles complexes. Première étude quasi-exhaustive des structures fines d’un genre et des propriétés optiques ainsi que colorimétriques générées, ce livre fournit aux entomologiste...

  2. Droit des organisations internationales

    CERN Document Server

    Sorel, Jean-Marc; Ndior, Valère

    2013-01-01

    Cet ouvrage collectif offre aux enseignants et chercheurs en droit international, aux praticiens et aux étudiants, une analyse actualisée du droit des organisations internationales. Il dresse en cinq parties un tableau, illustré par des exemples variés, des problématiques que soulève le phénomène polymorphe d institutionnalisation de la société internationale. La première partie est consacrée au phénomène des « organisations internationales », sous l angle à la fois de l institutionnalisation progressive des relations internationales et de la difficulté à cerner une catégorie unifiée. La deuxième partie rend compte de la création, de la disparition et des mutations des organisations internationales, ici envisagées comme systèmes institutionnels et ordres juridiques dérivés. La troisième partie analyse l autonomie que l acquisition de la personnalité juridique et de privilèges et immunités, un organe administratif intégré, un personnel ou un budget propres confèrent aux organi...

  3. Estimación de datos faltantes en estaciones meteorológicas de Venezuela vía un modelo de redes neuronales

    Directory of Open Access Journals (Sweden)

    Fernando Cedeño

    2008-10-01

    Full Text Available En el presente trabajo se propone un método de reconstrucción de series de tiempo de precipitaciones, para ser aplicado a las estaciones meteorológicas de Venezuela con el propósito de corregir el problema de datos faltantes. La metodología se fundamenta en dos técnicas: la primera reconstruye la dinámica y el tiempo de retardo del sistema dinámico de la serie temporal, y la segunda utiliza un modelo de redes neuronales para predecir los datos faltantes. Los modelos de redes neuronales exploran la dependencia espacio temporal de los atributos meteorológicos de las series y constituyen una herramienta importante para la propagación de la información relacionada con el clima, y además proveen soluciones prácticas de incertidumbre asociados con la interpolación y la captura de la estructura espacio temporal de los datos. Para llevar a cabo estos procedimientos, se ha determinado la dimensión de inmersión del atractor de las series y el tiempo de retardo, y luego se han usado estas medidas para definir la arquitectura de la red neuronal. El algoritmo utilizado para estimar los parámetros de la red neuronal ha sido el de retropropagación, que básicamente actualiza los pesos del modelo en la dirección en que el gradiente decrece más rápidamente. Para seleccionar la arquitectura de la red, se ha usado el criterio de información de Bayes (BIC, que consiste en penalizar el error cuadrático medio de los parámetros utilizados en el ajuste del modelo. Los resultados indican que las series de precipitaciones en Venezuela tienen alguna estructura subyacente no lineal, y provienen de un sistema caótico de bajas dimensiones. Los modelos de redes neuronales se han revelado útiles para la reconstrucción de los datos faltantes de las series.The present work proposes a method of reconstruction of precipitation time series, to be applied to the meteorological stations of Venezuela with the purpose of correcting the problem of missing data

  4. Predicción de la potencialidad de los bosques esclerófilos españoles mediante redes neuronales artificiales

    Directory of Open Access Journals (Sweden)

    Benito Garzón, M.

    2003-12-01

    Full Text Available Holm oak and cork oak forests are between the most important sclerophyllous formations in the Mediterranean Iberia. In order to study their potentiality, an artificial neural network model, with a feedforward BP algorithm, has been applied. The elevation, continentality, insolation, annual rainfall, annual mean temperature, mean temperature of the coldest month and mean temperature of the warmest month are the used bioclimatic variables with a 10 km resolution. The neural networks seem a highly predictive powerful tool. Different patterns in the response of the studied forests have been shown. The holm oak presents a continuous and wide potential simulate range. Meanwhile the cork oak potential area is fragmented and restricted, in accordance with its actual distribution area. The lack of both forests in the eastern and southern warm zones of Iberian Peninsula is the main discrepancy with previous potential vegetation proposals.

    Encinares y alcornocales son dos de las formaciones esclerófilas más importantes de la Iberia mediterránea. Para conocer cual es su potencialidad en el territorio español se ha empleado un modelo generado mediante redes neuronales artificiales con un algoritmo de retropropagación de errores que conduce la información siempre hacia delante. Las variables bioclimáticas empleadas como predictores son: altitud, continentalidad, insolación, precipitación total, temperatura media anual, temperatura media de las mínimas del mes más frío y temperatura media de las máximas del mes más cálido, con una resolución de 10 km. Las redes neuronales se perfilan como una herramienta de gran poder predictivo. Se aprecian patrones de respuesta diferente para las formaciones estudiadas. Mientras que para la encina se simula un área potencial continua y extensa, para el alcornoque se obtiene un área fragmentada y restringida, que se ajusta bastante a su presencia actual. La principal discrepancia del modelo presentado

  5. Dental Encounter System (DES)

    Data.gov (United States)

    Department of Veterans Affairs — Dental Encounter System (DES) is an automated health care application designed to capture critical data about the operations of VA Dental Services. Information on...

  6. Table des illustrations

    OpenAIRE

    2016-01-01

    Tableaux Dates d’inauguration des grands hôtels japonais entre 1860 et 1945… 19 Histoire, tourisme et hôtellerie en Corée depuis les années 1870… 59-60 Dates d’inauguration des grands hôtels chinois depuis 1863… 84 Les hôtels de luxe et leurs capacités d’hébergement en Corée en 2000… 103 Les flux de personnes suscités par les hôtels « super luxe » de Séoul en 2000… 105 L’activité des grands hôtels à Séoul en 1999 (en wons)… 106 Propriété et gestion des grands hôtels à Séoul en 1999…. 110 La c...

  7. Chimie des processus biologiques

    OpenAIRE

    Fontecave, Marc

    2010-01-01

    La chimie du vivant : enzymes et métalloenzymes, des biocatalyseurs fascinants La catalyse enzymatique, ce pouvoir que possèdent certaines protéines d’accélérer de façon extraordinaire et d’orienter avec une précision fascinante les réactions chimiques de la cellule, reste, encore aujourd’hui, l’un des grands mystères des systèmes vivants. Les réactions dont il est question vont de la simple hydrolyse de liaisons peptidiques à des processus poly-électroniques d’une très grande complexité, com...

  8. Vom Ort des Philosophierens

    OpenAIRE

    Damir Barbaric

    2002-01-01

    Im Aufsatz wird versucht, den ursprünglichen Sinn der Platonischen Bestimmung vom Staunen als Anfang der Philosophie zu ermitteln, und zwar durch eine kritischen Abhebung von ihrer Umdeutung bei Aristoteles, und dann insbesondere in der Stoa wie auch bei Spinoza und Hegel. Dabei wird auf die frühgriechische Erfahrung vom Staunen als _______ aufmerksam gemacht, wo es vor allem die Grundstimmung des Dabeiseins des Göttlichen und der menschlichen Teilnahme am Göttlichen bedeutet. Dem entspricht ...

  9. Liste des auteurs

    OpenAIRE

    2015-01-01

    Fariba Adelkhah est chargée de recherche à la Fondation nationale des sciences politiques, Centre d’études et de recherches internationales (CERI) [] Jean-Marie Bouissou est chargé de recherche à la Fondation nationale des sciences politiques, Centre d’études et de recherches internationales (CERI) [] Jean-Louis Briquet est chargé de recherche au CNRS, Centre d’études et de recherches internationales (CERI) [...

  10. La revolution des savants

    CERN Document Server

    Chavanne, A

    1989-01-01

    Premiere cassette : - 1666 : impact de la creation de l'Academie des Sciences par Colbert, trente ans apres le proces de Galile, et au moment des disparitions de Pascal, Descartes et Fermat. Elle dirigee par le hollandais Huyggens jusqu'a sa fuite de France au moment de la revocation de l'Edit de Nantes. - 1750 : l'Encyclopedie (ou "Dictionnaire raisonne des Sciences, des Arts et des Metiers") de Diderot et d'Alembert, soutenus par Malherbes, Buffon, Condorcet et Rousseau. - 1789 : Revolution francaise. - 8 aout 1793 : l'Assemblee, par une declaration de Marat, dissout l'Academie des Sciences. Celle-ci continue cependant ses travaux pour les poids et mesures jusqu'en 1795. - la Terreur : la condamnation a mort, pas au nom d'une "Revolution qui n'a pas besoin de savants" mais pour d'autres raisons, de trois grands hommes de science : Lavoisier, Bailly et Condorcet. - 1793-1794 : Au printemps 93, le Comite de Salut Publique s'inquiete du demi-million de soldats etrangers de toutes les pays frontaliers qui essai...

  11. Expression of Contractile Protein Isoforms in Microgravity

    Science.gov (United States)

    Anderson, Page A. W.

    1996-01-01

    The general objective of this experiment is to determine the effect of space flight parameters, including microgravity, on ontogenesis and embryogenesis of Japanese quail. Nine U.S. and two Russian investigators are cooperating in this study. Specific objectives of the participating scientists include assessing the gross and microscopic morphological and histological development of the embryo, as well as the temporal and spacial development of specific cells, tissues, and organs. Temporally regulated production of specific proteins is also being investigated. Our objective is to determine the effects of microgravity on developmentally programmed expression of Troponin T and I isoforms known to regulate cardiac and skeletal muscle contraction.

  12. Le dialogisme intertextuel des contes des Grimm

    OpenAIRE

    2013-01-01

    Loin d'être « purement allemands », nombre de contes des Grimm réécrivent les contes français largement diffusés en Allemagne au XVIIIe siècle en les « reconfigurant » selon leurs propres paradigmes esthétiques et idéologiques. L'étude introductive de la comparatiste Ute Heidmann montre que ce dialogisme européen est resté peu exploré en raison d’une « scénographie en trompe-l’œil » qui les présente comme issus du « terroir » hessois. Toutefois, les notes des Grimm (constituées en volume auto...

  13. Sobre la utilización del análisis de fourier, análisis espectral singular y redes neuronales artificiales en estratigrafía. parte 2: metodologías de análisis espectral en las formaciones tablazo – simití, sección sáchica (boyacá), colombia

    OpenAIRE

    García Cabrejo, Oscar Javier; Moreno Sánchez, Giovanni

    2014-01-01

    En este trabajo se presentan los resultados de la aplicación del Análisis de Fourier, AnálisisEspectral Singular Tradicional y Neuronal, y Redes Neuronales Artificiales para el análisis deinformación estratigráfica en una sección perteneciente a las formaciones Tablazo y Simití en cercaníasdel municipio de Sáchica, Boyacá, Colombia. Los resultados obtenidos indican que la combinaciónde Redes Neuronales de tipo Hebbiano y el Clasificador de Señal Múltiple permiten discriminar ciclosque no dete...

  14. Maladie des vibrations

    Science.gov (United States)

    Shen, Shixin (Cindy); House, Ronald A.

    2017-01-01

    Résumé Objectif Permettre aux médecins de famille de comprendre l’épidémiologie, la pathogenèse, les symptômes, le diagnostic et la prise en charge de la maladie des vibrations, une maladie professionnelle importante et courante au Canada. Sources d’information Une recherche a été effectuée sur MEDLINE afin de relever les recherches et comptes rendus portant sur la maladie des vibrations. Une recherche a été effectuée sur Google dans le but d’obtenir la littérature grise qui convient au contexte canadien. D’autres références ont été tirées des articles relevés. Message principal La maladie des vibrations est une maladie professionnelle répandue touchant les travailleurs de diverses industries qui utilisent des outils vibrants. La maladie est cependant sous-diagnostiquée au Canada. Elle compte 3 éléments : vasculaire, sous la forme d’un phénomène de Raynaud secondaire; neurosensoriel; et musculosquelettique. Aux stades les plus avancés, la maladie des vibrations entraîne une invalidité importante et une piètre qualité de vie. Son diagnostic exige une anamnèse minutieuse, en particulier des antécédents professionnels, un examen physique, des analyses de laboratoire afin d’éliminer les autres diagnostics, et la recommandation en médecine du travail aux fins d’investigations plus poussées. La prise en charge consiste à réduire l’exposition aux vibrations, éviter les températures froides, abandonner le tabac et administrer des médicaments. Conclusion Pour assurer un diagnostic rapide de la maladie des vibrations et améliorer le pronostic et la qualité de vie, les médecins de famille devraient connaître cette maladie professionnelle courante, et pouvoir obtenir les détails pertinents durant l’anamnèse, recommander les patients aux cliniques de médecine du travail et débuter les demandes d’indemnisation de manière appropriée. PMID:28292812

  15. Desarrollo de Experimentos de un Robot Navegador con Redes Neuronales en un Ambiente Estructurado: Programación de Conductas con Algoritmo de Retropropagación

    Directory of Open Access Journals (Sweden)

    Alejandro Hossian

    2015-02-01

    Full Text Available El presente artículo busca comunicar los avances realizados en el análisis y desarrollo de experimentos realizados en computadora para robots navegadores mediante la aplicación de Tecnologías Inteligentes. Para su realización se dispone de software y hardware apropiados a partir de los cuales se diseña un ambiente de navegación con obstáculos definidos en el marco de un entorno industrial. En este contexto, se diseña un ambiente computarizado con el cual el robot interactúa, en el cual se desplaza hasta llegar a un objetivo predeterminado, tratando de no colisionar. Bajo estas premisas y requisitos de usuario, se analiza el desempeño del robot a los efectos de procurar optimizar su performance dentro de este escenario. Para evaluar las potencialidades y limitaciones que presentan las conductas del robot, y en función de la complejidad del ambiente de navegación y de las tareas que éste debe llevar a cabo, se hace uso de las tecnologías de las Redes Neuronales Artificiales (RNA. A tal efecto, una arquitectura de RNA de tipo Backpropagation es la que se utiliza en el presente trabajo.

  16. Recognition of faults patterns in electric generators using artificial neurons networks; Reconocimiento de patrones de fallas en generadores electricos empleando redes neuronales artificiales

    Energy Technology Data Exchange (ETDEWEB)

    Rocha Sanchez, Martha Alicia

    1999-03-01

    This work present the development of a reprocessing method to reduce the information of the original data and to maintain the essential information of the data that enter the reduction process. The obtaining of these data is performed with the aid of the ICM ++ (ICMsystems), from which vectors or n-uplos of elements are obtained. This investigation allowed to analyze an evaluation of the paradigms of artificial neural networks, with the intention of detecting which of these paradigms would evolve better with the problem of fault diagnosis in electric generators. From this a prototype system was developed called diagnosis of faults in electrical generators, which automatically will recognize faults in electrical generators by means of the interpretation of the recording of partial discharges. [Spanish] El presente trabajo presenta el desarrollo de un metodo de reprocesamiento para reducir informacion de los datos originales y mantener la informacion esencial de los datos que entran al proceso de reduccion. La obtencion de estos datos se realiza con la ayuda del ICM ++ (ICMsystems), de los cuales se obtienen vectores o n-uplos de elementos. Esta investigacion permitio analizar una evaluacion de los paradigmas de redes neuronales artificiales, con el objeto de detectar cual de estos paradigmas se desempenaria mejor con el problema de diagnostico de fallas en generadores electricos. A partir de esto se desarrollo un sistema prototipo llamado diagnostico de fallas en generadores electricos, el cual automaticamente reconocera fallas en los generadores electricos mediante la interpretacion de registro de descargas parciales.

  17. Aplicación de un modelo de redes neuronales en la identificación del daño estructural en un puente vehicular

    Directory of Open Access Journals (Sweden)

    Carlos Alberto González Pérez

    2008-01-01

    Full Text Available En este artículo se presenta el desarrollo y aplicación de un modelo de redes neuronales en la detección del daño estructural a flexión en las trabes de un puente vehicular. El entrenamiento y evaluación de las redes se llevó a cabo a partir de la generación de 12 801 y 2 560 escenarios de daño “artificiales”, respectivamente. En la generación de estos escenarios se modificó aleatoriamente la rigidez a flexión de uno o varios de los elementos en que se discretizaron las trabes del puente. Como parámetro de entrada en el entrenamiento de las redes se utilizaron las diferencias en la energía de deformación modal y, como parámetro de salida, las rigideces a flexión de los elementos en que se discretizaron las trabes del puente. El algoritmo de entrenamiento utilizado fue el Scaled Conjugate Gradient. En general, se observó que las redes son capaces de predecir en forma confiable la localización y severidad del daño

  18. Androgen receptor isoforms in human and rat prostate

    Institute of Scientific and Technical Information of China (English)

    Shu-JieXIA; Gang-YaoHAO; Xiao-DaTANG

    2000-01-01

    Aim: To investigate the androgen receptor (AR) isoforms and its variability of expression in human and rat prostatic tissues. Methods: Human benign prostatic hyperplasia (BPH) and prostatic cancer tissues were obtained from patients undergoing prostatectomy, and rat ventral prostate was incised 3 days after castration. Forty-one AR-positive BPH specimens, 3 prostatic cancer specimens, and 6 rat prostates were used. After processing at 4℃, the tissues were examined by means of high resolution isoelectric focusing (IEF) technique to determine their AR isoforms. Results:From the prostatic specimens, 3 types of AR isoforms were detected with pI values at 6.5, 6.0, and 5.3. In human BPH tissues, 15/41 (36.6%) specimens showed all the three types of isoforms, while 19/41 (46.3%) showed 2 isoforms at various combinations and 7/41(17.1%), 1 isoform. For the 3 prostatic cancer specimens, one showed 3 isoforms, one, 2 isoforms, and the other failed to show any isoform. All rat prostatic tissues showed 2 isoforms at different combinations. Binding of 3H-dihydrotestosterone (DHT) to the isoforms was inhibited by the addition of 100-fold excess of DHT or testosterone, but not progesterone, oestradiol or diethylstilboestrol. Conclusion: AR isoforms are different in different patients. Although their genesis is not clear, the therapeutic implication of the present observation appears to be interesting, that may help clarifying the individual differences in the response to hormonal therapy.(Asian J Androl 2000 Dec;2:307-310)

  19. La convergence des rôles respectifs des relationnistes et des journalistes influence-t-elle la perception qu'ils ont les uns des autres?

    DEFF Research Database (Denmark)

    Valentini, Chiara

    2017-01-01

    la convergence des rôles respectifs des praticiens des relations publiques et des journalistes a un effet favorable sur la perception qu’ils ont les uns des autres. L’effet est plus marqué chez les praticiens des relations publiques, car leur vision de la profession en journalisme correspond à celle...

  20. The landscape of isoform switches in human cancers

    DEFF Research Database (Denmark)

    Vitting-Seerup, Kristoffer; Sandelin, Albin Gustav

    2017-01-01

    developed methods for identification and visualization of isoform switches with predicted functional consequences. Using these methods, we characterized isoform switching in RNA-seq data from >5,500 cancer patients covering 12 solid cancer types. Isoform switches with potential functional consequences were...... highly predictive of patient survival independent of cancer types. Our data constitute an important resource for cancer researchers, available through interactive web tools. Moreover, our methods, available as an R package, enable systematic analysis of isoform switches from other RNA-seq datasets...

  1. À propos des occasionnalismes

    Directory of Open Access Journals (Sweden)

    Dal Georgette

    2016-01-01

    Full Text Available Les occasionnalismes (nonce formations ou contextual formations dans la terminologie anglo-saxonne, qu’on définira provisoirement comme de “new complex word[s] created by a speaker/writer on the spur of the moment to cover some immediate need” (Bauer, 1983 : 45 ont, à notre connaissance, peu retenu l’attention des morphologues du domaine francophone. Pourtant, toutes les conditions sont désormais réunies pour que cet objet, invisible lorsqu’il s’agissait de décrire le système morphologique du français (ou d’autres langues à partir de ressources dictionnairiques, émerge en tant qu’observable dans une morphologie puisant ses données dans le réel langagier des locuteurs. Par définition en effet, on s’attend à ce qu’un occasionalisme soit absent des dictionnaires (nous verrons que, dans les faits, la situation est plus complexe que cela, et que ces contextual formations ne puissent pas être étudiées en dehors du contexte dans lequel elles ont été produites. À cet égard, la Toile et les produits qui en dérivent constituent des ressources de choix. C’est particulièrement vrai des forums, dans lesquels les internautes s’expriment librement, laissant libre cours à leur potentiel créatif (ou ce qu’ils pensent tel. Dans la présente communication, après avoir défini la notion d’occasionalisme, nous utiliserons un corpus constitué au fil d’autres recherches pour dégager des motifs récurrents propices à leur apparition, autrement dit pour établir une grammaire des occasionnalismes.

  2. Des Vents et des Jets Astrophysiques

    Science.gov (United States)

    Sauty, C.

    well expected result from the theory. Although, collimation may be conical, paraboloidal or cylindrical (Part 4), cylindrical collimation is the more likely to occur. The shape of outflows may then be used as a tool to predict physical conditions on the flows or on their source. L'éjection continue de plasma autour d'objets massifs est un phénomène largement répandu en astrophysique, que ce soit sous la forme du vent solaire, de vents stellaires, de jets d'étoiles en formation, de jets stellaires autour d'objets compacts ou de jets extra-galactiques. Cette zoologie diversifiée fait pourtant l'objet d'un commun effort de modélisation. Le but de cette revue est d'abord de présenter qualitativement le développement, depuis leur origine, des diverses théories de vents (Partie 1) et l'inter disciplinarité dans ce domaine. Il s'agit d'une énumération, plus ou moins exhaustive, des idées proposées pour expliquer l'accélération et la morphologie des vents et des jets, accompagnée d'une présentation sommaire des aspects observationnels. Cette partie s'abstient de tout aspect faisant appel au formalisme mathématique. Ces écoulements peuvent être décrits, au moins partiellement, en résolvant les équations magnétohydrodynamiques, axisymétriques et stationnaires. Ce formalisme, à la base de la plupart des théories, est exposé dans la Partie 2. Il permet d'introduire quantitativement les intégrales premières qu'un tel système possède. Ces dernières sont amenées à jouer un rôle important dans la compréhension des phénomènes d'accélération ou de collimation, en particulier le taux de perte de masse, le taux de perte de moment angulaire ou l'énergie du rotateur magnétique. La difficulté de modélisation réside dans l'existence de points critiques, propres aux équations non linéaires, qu'il faut franchir. La nature physique et la localisation de ces points critiques fait l'objet d'un débat important car ils sont la clef de voute de la r

  3. Aplicación de las Redes Neuronales al Pronóstico de Precios en el Mercado de Valores Application of Artificial Neural Networks to Price Forecasting in the Stock Exchange Market

    Directory of Open Access Journals (Sweden)

    Fernando Villada

    2012-01-01

    Full Text Available Este trabajo propone un modelo basado en redes neuronales artificiales para el pronóstico de los precios de dos de las principales acciones transadas en mercado de valores colombiano. El modelo propuesto se aplica al estudio de las acciones de Ecopetrol y Preferencial Bancolombia, empresas que negocian en las bolsas de valores de Colombia y Nueva York. Se utilizan dos estructuras de redes incluyendo como entradas la serie de precios diarios en la primera y la serie de precios más el índice del dólar estadounidense DXY en la segunda. Se prueban diferentes configuraciones de redes neuronales utilizando una serie de seis meses, donde los datos de los primeros cinco se utilizan para entrenamiento dejando el último mes para verificar la capacidad predictiva de la red. Los resultados muestran un buen comportamiento de las redes neuronales con bajos errores en su desempeño tanto en aprendizaje como en predicción.An artificial neural network model to forecast the price of two of the main shares traded in the Colombian stock exchange is proposed in this work. The model is applied to study the shares of Ecopetrol and Preferencial Bancolombia, companies that trade in the stock exchanges of Colombia and New York. Two network structures including the daily price series in the first and the price series plus the dollar index DXY in the latter are used. Different neural networks configurations are trained using a series of six months, where five months are used as training patterns and the next month is left to test the predictive capabilities of the network. The results show good performance of the neural networks with low training and testing errors.

  4. Análisis predictivo basado en redes neuronales no supervisadas aplicando algoritmo de kmedias y crisp-dm para pronóstico de riesgo de morosidad de los alumnos en la Universidad Peruana Unión

    OpenAIRE

    Pacco Palomino, Rodolfo

    2015-01-01

    El presente trabajo de investigación de tesis desarrolla los indicadores de gerencia financiera, capturados de la necesidad de los clientes, éstos son modelados y desarrollados a través de las tecnologías de BA (Business Analytics), las cuales tienen el objetivo de mostrar los riesgos de morosidad. Este proyecto de investigación se ha desarrollado basado sobre redes neuronales y la metodología CRISP-DM, para implementar e implantar el proyecto de BA (Business Analytics). Se ha hecho una optim...

  5. Redes neuronales y algoritmos genéticos en la docencia en las escuelas de ingenieros: Un problema práctico de diseño de pantallas acústicas

    Directory of Open Access Journals (Sweden)

    José Francisco Capdevila

    2010-06-01

    Full Text Available En este trabajo desarrollamos una técnica basada en redes neuronales y algoritmos genéticos para construir un instrumento matemático para el diseño de pantallas acústicas adaptadas. Para hacer esto, usamos la información de una empresa especializada en la construcción de estos dispositivos. En realidad, solucionamos el problema siguiente: si se quiere atenuar el ruido en un cierto punto, ¿cuál es la mejor pantalla para hacerlo?

  6. Aplicación informática de modelización del comportamiento de un motor, mediante la utilización de Redes Neuronales Artificiales (RNA)

    OpenAIRE

    Santolaria Castellanos, Ignacio

    2010-01-01

    El presente proyecto tiene como objetivo desarrollar un modelo de cálculo y control basado en una simulación termodinámica cero-dimensional de la fase cerrada – compresión, combustión y expansión de la mezcla aire-combustible – de un motor alternativo de combustión interna (MACI) de ciclo Otto. En primer lugar el estudio se centra en las redes neuronales, en sus diferentes tipologías y algoritmos de convergencia. Dicho modelo tiene en cuenta los principales parámetros que in...

  7. Alteraciones en la degradación intracelular de proteínas y en la endocitosis en las lipofuscinosis ceroideas neuronales infantil tardía y juvenil

    OpenAIRE

    Vidal Donet, José Manuel

    2014-01-01

    Las lipofuscinosis ceroideas neuronales son un grupo heterogéneo de enfermedades neurodegenerativas hereditarias graves que tienen su inicio en la infancia y que se engloban dentro de las enfermedades de almacenamiento lisosomal. Las características clínicas de estas patologías son una disminución de la capacidad mental acompañada de alteraciones motoras, epilepsia y pérdida de visión. Histológicamente las células muestran una acumulación de material autofluorescente (lipofuscina) en el inte...

  8. Detección y caracterización automáticas basadas en tratamiento digital de imágenes y redes neuronales de señales tonales emitidas por cetáceos

    OpenAIRE

    Sánchez, Antonio; Rodrígo, F. Javier; García, Pedro J; Bueno, Andrés; Muñoz,Patricio; Morales Sánchez, Juan; Larrey Ruiz, Jorge; Bastida Jumilla, María Consuelo; Sancho Gómez, José Luis

    2011-01-01

    Se presenta un nuevo método para la detección y caracterización de señales tonales emitidas por cetáceos. Las señales son atuomáticamente detectadas a partir del espectrograma, aisladas mediante un proceso de segmentación basado en regiones, extraídas tras un proceso de resolución de cruces y caracterizadas automáticamente mediante un número fijo de coeficientes derivados de la aplicación de redes neuronales de funciones de base radial. Estos coeficientes pueden ser empelados para una posteri...

  9. Detección y caracterización automáticas basadas en tratamiento digital de imágenes y redes neuronales de señales tonales emitidas por cetáceos

    OpenAIRE

    Sánchez, Antonio; Rodrígo, F. Javier; García, Pedro J.; Bueno, Andrés; Muñoz,Patricio; Morales Sánchez, Juan; Larrey Ruiz, Jorge; Bastida Jumilla, María Consuelo; Sancho Gómez, José Luis

    2011-01-01

    Se presenta un nuevo método para la detección y caracterización de señales tonales emitidas por cetáceos. Las señales son atuomáticamente detectadas a partir del espectrograma, aisladas mediante un proceso de segmentación basado en regiones, extraídas tras un proceso de resolución de cruces y caracterizadas automáticamente mediante un número fijo de coeficientes derivados de la aplicación de redes neuronales de funciones de base radial. Estos coeficientes pueden ser empelados para una posteri...

  10. Sobre el uso de técnicas chopper para la reducción del ruido flicker en amplificadores para la captación de señales neuronales

    OpenAIRE

    Pérez Prieto, Norberto

    2016-01-01

    La captación de señales neuronales mediante electrodos conectados a circuitos micro-electrónicos es necesaria para aplicaciones clínicas y para el control de prótesis senso-motoras, entre otras muchas aplicaciones bio-médicas. En todas estas aplicaciones, la preservación de la información contenida en las imágenes captadas depende críticamente de las prestaciones de los amplificadores empleados en la cabecera de la cadena de procesamiento electrónica. El problema es que se trata de señales mu...

  11. Redes Neuronales para predecir el rendimiento académico de los alumnos ingresantes a la carrera de Bioquímica de la FACENA-UNNE en función de sus conocimientos matemáticos previos

    OpenAIRE

    López,María Victoria; Ramírez Arballo, M. G.; Porcel, Eduardo; Mata, Liliana E.; Barreto, Silvia E.

    2012-01-01

    Este trabajo tiene por objetivo desarrollar modelos de redes neuronales artificiales (RNA) para analizar la incidencia de los conocimientos matemáticos previos de los alumnos ingresantes a la carrera de Bioquímica de la FACENA-UNNE, en su rendimiento académico en el primer cuatrimestre del primer año. Se analizaron los datos del estado académico de los alumnos y de sus conocimientos matemáticos previos, siendo obtenidos estos últimos a partir de una evaluación diagnóstica, implementada en 200...

  12. Table des illustrations

    OpenAIRE

    2015-01-01

    Intégrer les Tsiganes Hongrie, 1970 (photo DR) 34 Discuter entretient la fraternité. (photo M. Stewart) 35 La veille du mariage (photo M. Stewart) 36 Le « salon » (photo M. Stewart) 37 Les activités économiques des Tsiganes sont conçues comme des jeux. (photo M. Stewart) 39 Lev cheval (photo I. Nemeth) 41 Le cortège d’un mariage (photo M. Stewart) 43 Avant le mariage (photo M. Stewart) 43 C’est par le chant (photo M. Stewart) 45 Des « frères » boivent en harmonie (photo M. Stewart) 46 Les che...

  13. Geschichte des SIN

    CERN Document Server

    Pritzker, Andreas

    2013-01-01

    Dieses Buch erzählt die Geschichte des Schweizerischen Instituts für Nuklearforschung (SIN). Das Institut wurde 1968 gegründet und ging 1988 ins Paul Scherrer Institut (PSI) über. Die Gründung des SIN erfolgte in einer Zeit, als die Physik weitherum als Schlüsseldisziplin für die technologische und gesellschaftliche Entwicklung galt. Der Schritt war für ein kleines Land wie die Schweiz ungewöhnliche und zeugte von Mut und Weitsicht. Ungewöhnlich waren der Folge die Leistungen des SIN im weltweiten Vergleich sowie sein Einfluss auf die schweizerische, teils auf die internationale Wissenschaftspolitik.

  14. Les lueurs des sables

    CERN Multimedia

    Les lueurs des sables

    2013-01-01

    Two CERN ladies are getting ready for the “Trophée Roses des Sables” rally adventure: Julie and Laetitia are finalizing the last details before setting off on Monday 7th October 2013. Julie from EN-MEF group and Laetitia from DGS-SEE group, met at the CERN Rugby club. This year, they are participating in the 100 % female rally which will take place in Morocco from 10 to 20 October. They will be carrying along 100 kg of humanitarian donation for children such as some clothes, books and medical material. Do not hesitate to show your support at their farewell party to be held on Monday 7 October, from 4 to 6 pm in front of the St Genis-Pouilly Mairie (city Hall). Follow their exciting adventure on the blog leslueursdessables.trophee-roses-des-sables.org and on their association’s Facebook page Les Lueurs des Sables.

  15. Des Groupes et des Œuvres

    OpenAIRE

    Grojnowski, Daniel

    2015-01-01

    À la fin du xixe siècle, les groupes de bohèmes se multiplient et se diversifient, des salons privés aux tavernes ou cabarets. Une étude aussi attentive que possible de trois d’entre eux (les Zutiques, les Hydropathes, les Incohérents) montre la diversité de leurs configurations, ainsi que l’importance déterminante que leurs productions revêt au regard de la postérité.

  16. Differentialdiagnose des Ulcus cruris

    Directory of Open Access Journals (Sweden)

    Binder B

    2010-01-01

    Full Text Available Das Ulcus cruris ist eine Erkrankung vor allem des höheren Lebensalters. Ungefähr 1 % der Bevölkerung leidet an einer chronischen Wunde. An die 90 % der Ulzera sind vaskulärer Genese, doch gibt es vielfältige weitere Ursachen. Die Kenntnis der relevanten Differentialdiagnosen ist insbesondere bei therapierefraktären Verläufen von Bedeutung. Dazu gehören Neuropathien, myeloproliferative Erkrankungen, Infektionen, Medikamente oder Hauttumore. Für eine erfolgreiche Behandlung des Ulcus cruris ist die Identifikation der Genese von entscheidender Bedeutung, um eine kausale, interdisziplinäre Therapie zu ermöglichen.

  17. Liste des Auteurs

    OpenAIRE

    2014-01-01

    Amor Hakima, Université Cadi Ayyad, Faculté des sciences Semlalia, Département de biologie, Laboratoire d’écologie humaine, Marrakech, Maroc. Angeli Aurora, Universita degli studi di Bologna, Dipartimento di scienze statiche “Paolo Fortunati”, Bologne, Italie. Baali Abdellatif, Université Cadi Ayyad, Faculté des sciences Semlalia, Département de biologie, Laboratoire d’écologie humaine, Marrakech, Maroc. Bachtarzi T., Hôpital Ibn Sina, CHU Annaba, Algérie. Baudot Patrick, Université de Proven...

  18. Liste des Auteurs

    OpenAIRE

    2014-01-01

    Amor Hakima, Université Cadi Ayyad, Faculté des sciences Semlalia, Département de biologie, Laboratoire d’écologie humaine, Marrakech, Maroc. Angeli Aurora, Universita degli studi di Bologna, Dipartimento di scienze statiche “Paolo Fortunati”, Bologne, Italie. Baali Abdellatif, Université Cadi Ayyad, Faculté des sciences Semlalia, Département de biologie, Laboratoire d’écologie humaine, Marrakech, Maroc. Bachtarzi T., Hôpital Ibn Sina, CHU Annaba, Algérie. Baudot Patrick, Université de Proven...

  19. Isoforms of Melanopsin Mediate Different Behavioral Responses to Light

    Science.gov (United States)

    Jagannath, Aarti; Hughes, Steven; Abdelgany, Amr; Pothecary, Carina A.; Di Pretoro, Simona; Pires, Susana S.; Vachtsevanos, Athanasios; Pilorz, Violetta; Brown, Laurence A.; Hossbach, Markus; MacLaren, Robert E.; Halford, Stephanie; Gatti, Silvia; Hankins, Mark W.; Wood, Matthew J.A.; Foster, Russell G.; Peirson, Stuart N.

    2015-01-01

    Summary Melanopsin (OPN4) is a retinal photopigment that mediates a wide range of non-image-forming (NIF) responses to light [1, 2] including circadian entrainment [3], sleep induction [4], the pupillary light response (PLR) [5], and negative masking of locomotor behavior (the acute suppression of activity in response to light) [6]. How these diverse NIF responses can all be mediated by a single photopigment has remained a mystery. We reasoned that the alternative splicing of melanopsin could provide the basis for functionally distinct photopigments arising from a single gene. The murine melanopsin gene is indeed alternatively spliced, producing two distinct isoforms, a short (OPN4S) and a long (OPN4L) isoform, which differ only in their C terminus tails [7]. Significantly, both isoforms form fully functional photopigments [7]. Here, we show that different isoforms of OPN4 mediate different behavioral responses to light. By using RNAi-mediated silencing of each isoform in vivo, we demonstrated that the short isoform (OPN4S) mediates light-induced pupillary constriction, the long isoform (OPN4L) regulates negative masking, and both isoforms contribute to phase-shifting circadian rhythms of locomotor behavior and light-mediated sleep induction. These findings demonstrate that splice variants of a single receptor gene can regulate strikingly different behaviors. PMID:26320947

  20. Tocopherol isoforms in parenteral lipid emulsions and neutrophil activation.

    NARCIS (Netherlands)

    Wanten, G.J.A.; Beunk, J.; Naber, A.H.J.; Swinkels, D.W.

    2002-01-01

    BACKGROUND AND AIMS: Tocopherol is a lipid-soluble anti-oxidant that exists in several isoforms. Patients on total parenteral nutrition depend on lipid emulsions for their tocopherol intake. In the present study, we analysed the content of tocopherol isoforms in various lipid emulsions. We also

  1. Evidence for leptin receptor isoforms heteromerization at the cell surface.

    Science.gov (United States)

    Bacart, Johan; Leloire, Audrey; Levoye, Angélique; Froguel, Philippe; Jockers, Ralf; Couturier, Cyril

    2010-06-01

    Leptin mediates its metabolic effects through several leptin receptor (LEP-R) isoforms. In humans, long (LEPRb) and short (LEPRa,c,d) isoforms are generated by alternative splicing. Most of leptin's effects are believed to be mediated by the OB-Rb isoform. However, the role of short LEPR isoforms and the possible existence of heteromers between different isoforms are poorly understood. Using BRET1 and optimized co-immunoprecipitation, we observed LEPRa/b and LEPRb/c heteromers located at the plasma membrane and stabilized by leptin. Given the widespread coexpression of LEPRa and LEPRb, our results suggest that LEPRa/b heteromers may represent a major receptor species in most tissues.

  2. Localization and functional characterization of the human NKCC2 isoforms

    DEFF Research Database (Denmark)

    Carota, I; Theilig, F; Oppermann, M;

    2010-01-01

    AIM: Salt reabsorption across the apical membrane of cells in the thick ascending limb (TAL) of Henle is primarily mediated by the bumetanide-sensitive Na(+)/K(+)/2Cl(-) cotransporter NKCC2. Three full-length splice variants of NKCC2 (NKCC2B, NKCC2A and NKCC2F) have been described. The NKCC2...... isoforms have specific localizations and transport characteristics, as assessed for rabbit, rat and mouse. In the present study, we aimed to address the localization and transport characteristics of the human NKCC2 isoforms. METHODS: RT-PCR, in situ hybridization and uptake studies in Xenopus oocytes were...... performed to characterize human NKCC2 isoforms. RESULTS: All three classical NKCC2 isoforms were detected in the human kidney; in addition, we found splice variants with tandem duplicates of the variable exon 4. Contrary to rodents, in which NKCC2F is the most abundant NKCC2 isoform, NKCC2A was the dominant...

  3. Development and characterization of human monoclonal antibodies that neutralize multiple TGFβ isoforms

    National Research Council Canada - National Science Library

    Bedinger, Daniel; Lao, Llewelyn; Khan, Shireen; Lee, Steve; Takeuchi, Toshihiko; Mirza, Amer M

    .... There are 3 main isoforms, TGFβ1, 2, and 3. As multiple TGFβ isoforms are involved in disease processes, maximal therapeutic efficacy may require neutralization of 2 or more of the TGFβ isoforms...

  4. able utilizando redes neuronales artificiales; UTILIZATION OF ARTIFICIAL NEURAL NETWORK IN THE SIMULATION AND CONTROL OF WIND TURBINE GENERATORS WITH VARIABLE SPEED AND VARIABLE PITCH.

    Directory of Open Access Journals (Sweden)

    Osley López González

    2011-02-01

    Full Text Available Con el objetivo de aprovechar al máximo la energía del viento y, a la vez, llevar a cabo un control rápido ypreciso de la potencia máxima suministrada al aumentar la misma se han venido utilizando cada vez mássistemas de control capaces de operar en el punto óptimo de entrega de potencia para determinadosvalores de velocidad del viento y limitarla cuando éste supera su valor máximo. Este sistema de control,considerado en su conjunto, debe responder con la exactitud, estabilidad y rapidez necesaria ante lavariabilidad y aleatoriedad del viento. La relación entre sus variables de salida (velocidad de la turbina ypaso de la pala y la de entrada (velocidad del viento que sea capaz de aprovechar la máxima potenciadisponible en el viento en una zona de trabajo y de limitarla en otra, es altamente complicada puesdepende de factores constructivos y de diseño de la turbina y del generador. Esta característica es muydifícil de representar (sea con el objetivo de simulación o con el de control mediante relacionesfuncionales matemáticas convencionales.En este trabajo los autores proponen representar dicha relaciónmediante la utilización de Redes Neuronales Artificiales entrenadas para ser capaces de responderadecuadamente ante cualquier entrada. Basados en los parámetros y características de un aerogeneradorreal de velocidad y paso variables y utilizando el toolbox de Redes Neuronales del MATLAB SIMULINK losautores obtuvieron un modelo neuronal del sistema de control de la velocidad y el paso de la turbinacomprobando su correcta operación ante diferentes perturbaciones de la red eléctrica mediante estemismo lenguaje de simulación. Se demostró que estas redes pueden ser utilizadas con éxito en lasimulación y el control de este tipo de máquinas en cualquier condición de operación. In order to capture the maximum energy from the wind, control systems operating always at an optimumpower has been utilized. This control system

  5. Die lebensvision des semonides

    Directory of Open Access Journals (Sweden)

    Milivoj Sironić

    1980-12-01

    Full Text Available Der Autor analysiert die Weibersatire des Semonides und andere seiner kurzen Fragmente. Dabei kommt er zu der Schlussfolgerung, dass sich die Verse der Satire durch eine gute Technik auszeich­ nen, dass aber auch eine unnötige Weitschweifigkeit nicht zu Ubersehen ist. Trotzdem kann man jedoch dem Dichter nicht die künstlerische Überzeugungskraft absprechen, wie auch die Ten­ denz nach Detailschilderungen und die Feinheit geistreicher Charakterisierungen. Die Aussage beinhaltet manchmal eine be­ stimmte innere Ironie in der Einfachheit der Wörter.In kurzen Fragmenten wird die Fähigkeit des Semonides sichtbar, sich sehr präzise auszudrücken. Seine ironisch-epische Sprache ist sehr reich  und verdient vom linguistischen Standpunkt aus Auf­ merksamkeit wegen ihres reichhaltigen lexischen und termino­ logischen Materials, insbesondere was die Bereiche der Tier­ welt und des Essens anbelangt.Die Weltanschauung des Dichters ist in dem ersten Fragment recht deutlich erkennbar. Da sieht man, dass er düster und pessimistisch auf das Leben blickt. Das Fehlen jeglicher positiven Einstellung hat dann den Sar­ kasmus zur Folge, mit dem der Dichter die Darstellung der Weiber einkleidet, ihn aber auch auf die Menschen schlechthin ausweitet.

  6. La physique des infinis

    CERN Document Server

    Bernardeau, Francis; Laplace, Sandrine; Spiro, Michel

    2013-01-01

    Écrire l'histoire de l'Univers, tel est l'objectif commun des physiciens des particules et des astrophysiciens. Pour y parvenir, deux approches s'épaulent : la voie de l'infiniment petit, que l'on emprunte via de gigantesques accélérateurs de particules, et celle de l'infiniment grand, dont le laboratoire est l'Univers. Un Univers qui est bien loin d'avoir livré tous ses secrets. On connaît à peine 4,8 % de la matière qui le constitue, le reste étant composé de matière noire (25,8 %) et d'énergie noire (69,4 %), toutes deux de nature inconnue. Et si la récente découverte du boson de Higgs valide le Modèle standard de la physique des particules, celui-ci est toujours incomplet et doit être étendu à ou dépassé. Est-on arrivé au bout du jeu de poupées russes de la matière ? Quelles sont les particules manquantes ? Faut-il revoir les lois fondamentales ? Quels instruments faut-il mettre en œuvre pour accéder à cette « nouvelle physique » ? Comment parler de Super Big Science aux citoye...

  7. CHAINE DES ROTISSEUR @ HILTON

    Institute of Scientific and Technical Information of China (English)

    2005-01-01

    The annual gala dinner of Chaine des Rotisseurs was hosted by Hilton Beijing. The “Ballet Dinner” - a great creation for both art and fine dining lovers was taken place in varies places within the hotel, including hotel lobby, 3rd floor and lobby lounge.

  8. REPRISE DES COURS - Yoga

    CERN Multimedia

    Club de Yoga

    2015-01-01

    REPRISE DES COURS – Venez nombreux ! Yoga, Sophrologie, Tai Chi La liste des cours pour le semestre allant du 1er septembre 2015 au 31 janvier 2016 est disponible sur notre site web : http://club-yoga.web.cern.ch Lieu Les cours ont lieu dans la salle des clubs, à l’entresol du restaurant No 2, Bât. 504 (dans la salle no 3 pour la Sophrologie). Prix des cours Le prix pour le semestre (environ 18 leçons) est fixé à 220 CHF plus 10 CHF d’adhésion annuelle au Club. Couple : 200 CHF par personne. 2 cours par semaine : 400 CHF. Inscriptions Les inscriptions aux cours seront prises directement auprès du professeur, lors de la 1ère séance. Avant de s’inscrire pour le semestre, il est possible d’essayer une séance gratuitement. Informations : http://club-yoga.web.cern.ch ----------------------------------------- cern.ch/club-yoga/

  9. Potenziale des Mobile Commerce

    OpenAIRE

    Turowski, Klaus

    2004-01-01

    Potenziale des Mobile Commerce / B. Bazijanec, K. Turowski. - In: Mobile economy : 4. Workshop Mobile Commerce, 02.-03. Februar 2004, Univ. Augsburg / Key Pousttchi ... (Hrsg.). - Bonn : Ges. für Informatik, 2004. - S. 7-11. - (GI-Edition : Proceedings ; 42)

  10. Vom Ort des Philosophierens

    Directory of Open Access Journals (Sweden)

    Damir Barbaric

    2002-12-01

    Full Text Available Im Aufsatz wird versucht, den ursprünglichen Sinn der Platonischen Bestimmung vom Staunen als Anfang der Philosophie zu ermitteln, und zwar durch eine kritischen Abhebung von ihrer Umdeutung bei Aristoteles, und dann insbesondere in der Stoa wie auch bei Spinoza und Hegel. Dabei wird auf die frühgriechische Erfahrung vom Staunen als _______ aufmerksam gemacht, wo es vor allem die Grundstimmung des Dabeiseins des Göttlichen und der menschlichen Teilnahme am Göttlichen bedeutet. Dem entspricht die bei Platon stark hervorgehobene Rolle des “Pathetischen” am Staunen, das in der nachkommenden Tradition grundsätzlich verlorengegangen ist. Für Platon entspringt das Staunen einem nie zu überwindenden Zustand der wesentlichen philosophischen “Weglosigkeit” (_____, welche schon bei Aristoteles nur noch im Sinne eines unbedingt zu verlassenden Ausgangspunkts der methodisch sicher fortfahrenden philosophischen Wissenschaft verstanden wird. Durch ein Zurückgreifen auf die Diotimas Lehre im Dialog Symposion wird dementgegen die Sokratisch-Platonische “Weglosigkeit” als der einzig angemessene “Ort” des echten Philosophierens bestimmt, welchen es, als das “Zwischen” für das Weltspiel der Unsterblichen und Sterblichen, um jeden Preis zu bewahren und offen zu halten gilt.

  11. Análisis del electrocardiograma de la ballena jorobada con técnicas de procesamiento de señales y redes neuronales

    Directory of Open Access Journals (Sweden)

    Yolanda Plazas

    2000-02-01

    Full Text Available

    Se analizaron 199 complejos del electrocardiograma (ECG de ballena jorobada, con técnicas en el dominio del tiempo, de la frecuencia, identificación de sistemas. Se clasificaron con redes neuronales y análisis de cluster. Se obtuvo promedio típico del ECG de la ballena, se comprobó la existencia de la onda P, se dio una nueva explicación a la bradicardia de estos animales y se obtuvo un modelo matemático para simular el ECG.

    Metodología: Los complejos del ECG fueron obtenidos por el grupo S.C.V.S. Se obtuvo la señal promedio de los 199 complejos. Se realizó una correlación cruzada, entre el promedio y los 199 complejos para identificar y promediar los complejos más típicos. Se realizaron análisis espectrales de la señal promediada, de cada complejo y de segmentos. Se hizo un diezmado de la señal y una compresión con transformada discreta coseno. Se clasificaron las señales con redes neuronales tipo Kohonen, y análisis de cluster. Se hizo identificación de sistemas con modelos paramétricos tipo ARX, como entrada se tuvo un pulso rectangular de 300 milisegundos y como salida, la señal promediada. Se hallaron los polos, los ceros y la respuesta en frecuencia y al escalón del sistema. Se realizó una prueba de chi cuadrado para establecer la asociación entre la clasificación realizada por las redes neuronales y el análisis de cluster y un análisis de la varianza, para establecer la significancia de las diferencias encontradas entre los grupos.

    Resultados: La frecuencia cardíaca promedio fue de 7 +3 latidos por minuto. Se observaron ondas similares a la P del ECG humano

  12. Engineering of insulin receptor isoform-selective insulin analogues.

    Directory of Open Access Journals (Sweden)

    Tine Glendorf

    Full Text Available BACKGROUND: The insulin receptor (IR exists in two isoforms, A and B, and the isoform expression pattern is tissue-specific. The C-terminus of the insulin B chain is important for receptor binding and has been shown to contact the IR just adjacent to the region where the A and B isoforms differ. The aim of this study was to investigate the importance of the C-terminus of the B chain in IR isoform binding in order to explore the possibility of engineering tissue-specific/liver-specific insulin analogues. METHODOLOGY/PRINCIPAL FINDINGS: Insulin analogue libraries were constructed by total amino acid scanning mutagenesis. The relative binding affinities for the A and B isoform of the IR were determined by competition assays using scintillation proximity assay technology. Structural information was obtained by X-ray crystallography. Introduction of B25A or B25N mutations resulted in analogues with a 2-fold preference for the B compared to the A isoform, whereas the opposite was observed with a B25Y substitution. An acidic amino acid residue at position B27 caused an additional 2-fold selective increase in affinity for the receptor B isoform for analogues bearing a B25N mutation. Furthermore, the combination of B25H with either B27D or B27E also resulted in B isoform-preferential analogues (2-fold preference even though the corresponding single mutation analogues displayed no differences in relative isoform binding affinity. CONCLUSIONS/SIGNIFICANCE: We have discovered a new class of IR isoform-selective insulin analogues with 2-4-fold differences in relative binding affinities for either the A or the B isoform of the IR compared to human insulin. Our results demonstrate that a mutation at position B25 alone or in combination with a mutation at position B27 in the insulin molecule confers IR isoform selectivity. Isoform-preferential analogues may provide new opportunities for developing insulin analogues with improved clinical benefits.

  13. Injectabilite des coulis de ciment dans des milieux fissures

    Science.gov (United States)

    Mnif, Thameur

    Le travail presente ici est un bilan du travaux de recherche effectues sur l'injectabilite des coulis de ciment dans lu milieux fissures. Un certain nombre de coulis a base de ciment Portland et microfin ont ete selectionnes afin de caracteriser leur capacite a penetrer des milieux fissures. Une partie des essais a ete menee en laboratoire. L'etude rheologique des differents melanges a permis de tester l'influence de l'ajout de superplastifiant et/ou de fumee de silice sur la distribution granulometrique des coulis et par consequent sur leur capacite a injecter des colonnes de sable simulant un milieu fissure donne. La classe granulometrique d'un coulis, sa stabilite et sa fluidite sont apparus comme les trois facteurs principaux pour la reussite d'une injection. Un facteur de finesse a ete defini au cours de cette etude: base sur la classe granulometrique du ciment et sa stabilite, il peut entrer dans la formulation theorique du debit d'injection avant application sur chantier. La deuxieme et derniere partie de l'etude presente les resultats de deux projets de recherche sur l'injection realises sur chantier. L'injection de dalles de beton fissurees a permis le suivi de l'evolution des pressions avec la distance au point d'injection. L'injection de murs de maconnerie a caractere historique a montre l'importance de la definition de criteres de performance des coulis a utiliser pour traiter un milieu donne et pour un objectif donne. Plusieurs melanges peuvent ainsi etre predefinis et mis a disposition sur le chantier. La complementarite des ciments traditionnels et des ciments microfins devient alors un atout important. Le choix d'utilisation de ces melanges est fonction du terrain rencontre. En conclusion, cette recherche etablit une methodologie pour la selection des coulis a base de ciment et des pressions d'injection en fonction de l'ouverture des fissures ou joints de construction.

  14. Relación entre la creación de valor y la inversión en I+D: una aproximación mediante redes neuronales artificiales

    Directory of Open Access Journals (Sweden)

    Ángel Samaniego Alcántar

    2013-11-01

    Full Text Available Este documento proporciona nueva evidencia sobre la interacción de los efectos del tipo de interés a cinco años, el nivel de apalancamiento (la relación del pasivo y activo, las pérdidas históricas, el crecimiento del PIB, la intensidad del gasto en I+D y los ingresos con el rendimiento de la acción en el corto plazo. El modelo no lineal utilizado (combinación de modelos mediante redes neuronales artificiales explica 31.59% de la varianza del rendimiento de las acciones en los primeros 5 meses del año, entre 2000-2006, en los mercados AMEX, NASDAQ y NYSE. Existe evidencia en la literatura que el 32% de explicación es alto, en comparación al estudio de la literatura realizado por Coad (2009 donde se utilizan modelos lineales que buscan explicar el crecimiento de la empresa. Se observa que los modelos lineales explican entre el 17% al 32% de la variabilidad de los datos. Al igual que en los trabajos de Bode (1998, Tsai (2005, Wang y Chien (2006 y Chien et al. (2010 se utilizan las redes neuronales artificiales para el estudio de la interacción entre la I+D y la empresa.

  15. Adiponectin isoforms: a potential therapeutic target in rheumatoid arthritis?

    Science.gov (United States)

    Frommer, Klaus W; Schäffler, Andreas; Büchler, Christa; Steinmeyer, Jürgen; Rickert, Markus; Rehart, Stefan; Brentano, Fabia; Gay, Steffen; Müller-Ladner, Ulf; Neumann, Elena

    2012-10-01

    Several clinical studies have suggested the adipocytokine adiponectin is involved in the progression of rheumatoid arthritis (RA). From this point of view, adiponectin might present a new therapeutic target. However, as adiponectin also exerts beneficial effects in the human organism, a strategy that would allow its detrimental effects to be abolished while maintaining the positive effects would be highly favourable. To elucidate such a strategy, the authors analysed whether the different adiponectin isoforms induce diverging effects, especially with regard to rheumatoid arthritis synovial fibroblasts (RASF), a central cell type in RA pathogenesis capable of invading into and destroying cartilage. Affymetrix microarrays were used to screen for changes in gene expression of RASF. Messenger RNA levels were quantified by real-time PCR, protein levels by immunoassay. The migration of RASF and primary human lymphocytes was analysed using a two-chamber migration assay. In RASF, the individual adiponectin isoforms induced numerous genes/proteins relevant in RA pathogenesis to clearly different extents. In general, the most potent isoforms were the high molecular weight/middle molecular weight isoforms and the globular isoform, while the least potent isoform was the adiponectin trimer. The chemokines secreted by RASF upon adiponectin stimulation resulted in an increased migration of RASF and lymphocytes. The results clearly suggest a pro-inflammatory and joint-destructive role of all adiponectin isoforms in RA pathophysiology, indicating that in chronic inflammatory joint diseases the detrimental effects outweigh the beneficial effects of adiponectin.

  16. Comparison of Regression and Neural Networks Models to Estimate Solar Radiation Comparación de Regresión y Modelos de Redes Neuronales para Estimar la Radiación Solar

    Directory of Open Access Journals (Sweden)

    Mónica Bocco

    2010-09-01

    Full Text Available The incident solar radiation on soil is an important variable used in agricultural applications; it is also relevant in hydrology, meteorology and soil physics, among others. To estimate this variable, empirical models have been developed using several parameters and, recently, prognostic and prediction models based on artificial intelligence techniques such as neural networks. The aim of this work was to develop linear models and neural networks, multilayer perceptron, to estimate daily global solar radiation and compare their efficiency in its application to a region of the Province of Salta, Argentina. Relative sunshine duration, maximum and minimum temperature, rainfall, binary rainfall and extraterrestrial solar radiation data for the period 1996-2002, were used. All data were supplied by Experimental Station Salta, Instituto Nacional de Tecnología Agropecuaria (INTA, Argentina. For both, neural networks models and linear regressions, three alternative combinations of meteorological parameters were considered. Good results with both prediction methods were obtained, with root mean square error (RMSE values between 1.99 and 1.66 MJ m-2 d-1 for linear regressions and neural networks, and coefficients of correlation (r² between 0.88 and 0.92, respectively. Even though neural networks and linear regression models can be used to predict the daily global solar radiation appropriately, neural networks produced better estimates.La radiación solar incidente en el suelo es una variable importante usada en aplicaciones agronómicas, además es relevante en hidrología, meteorología y física del suelo, entre otros. Para estimarla se han desarrollado modelos empíricos que utilizan distintos parámetros meteorológicos y, recientemente, modelos de pronóstico y predicción basados en técnicas de inteligencia artificial tales como redes neuronales. El objetivo de este trabajo fue desarrollar modelos lineales y de redes neuronales, del tipo perceptr

  17. Nonmuscle myosin II isoforms coassemble in living cells.

    Science.gov (United States)

    Beach, Jordan R; Shao, Lin; Remmert, Kirsten; Li, Dong; Betzig, Eric; Hammer, John A

    2014-05-19

    Nonmuscle myosin II (NM II) powers myriad developmental and cellular processes, including embryogenesis, cell migration, and cytokinesis [1]. To exert its functions, monomers of NM II assemble into bipolar filaments that produce a contractile force on the actin cytoskeleton. Mammalian cells express up to three isoforms of NM II (NM IIA, IIB, and IIC), each of which possesses distinct biophysical properties and supports unique as well as redundant cellular functions [2-8]. Despite previous efforts [9-13], it remains unclear whether NM II isoforms assemble in living cells to produce mixed (heterotypic) bipolar filaments or whether filaments consist entirely of a single isoform (homotypic). We addressed this question using fluorescently tagged versions of NM IIA, IIB, and IIC, isoform-specific immunostaining of the endogenous proteins, and two-color total internal reflection fluorescence structured-illumination microscopy, or TIRF-SIM, to visualize individual myosin II bipolar filaments inside cells. We show that NM II isoforms coassemble into heterotypic filaments in a variety of settings, including various types of stress fibers, individual filaments throughout the cell, and the contractile ring. We also show that the differential distribution of NM IIA and NM IIB typically seen in confocal micrographs of well-polarized cells is reflected in the composition of individual bipolar filaments. Interestingly, this differential distribution is less pronounced in freshly spread cells, arguing for the existence of a sorting mechanism acting over time. Together, our work argues that individual NM II isoforms are potentially performing both isoform-specific and isoform-redundant functions while coassembled with other NM II isoforms.

  18. Dopage et protection des jeunes sportifs : Loin des affaires

    OpenAIRE

    Guy, Daniel

    2002-01-01

    National audience; Loin des affaires et des révélations polémiques de la presse, les jeunes plébiscitent massivement les activités sportives. Sport compétition, sport participation, sport détente... Pourtant, quand la pratique devient intense, que les entraînements et les compétitions s'enchaînent à un rythme soutenu, l'ombre des conduites dopantes vient déchirer la quiétude des uns et des autres. Or, que savons-nous réellement de la pratique des jeunes sportifs ? Comment préparent-ils les co...

  19. p53 Family: Role of Protein Isoforms in Human Cancer

    Directory of Open Access Journals (Sweden)

    Jinxiong Wei

    2012-01-01

    Full Text Available TP53, TP63, and TP73 genes comprise the p53 family. Each gene produces protein isoforms through multiple mechanisms including extensive alternative mRNA splicing. Accumulating evidence shows that these isoforms play a critical role in the regulation of many biological processes in normal cells. Their abnormal expression contributes to tumorigenesis and has a profound effect on tumor response to curative therapy. This paper is an overview of isoform diversity in the p53 family and its role in cancer.

  20. Targeted Proteomics Enables Simultaneous Quantification of Folate Receptor Isoforms and Potential Isoform-based Diagnosis in Breast Cancer.

    Science.gov (United States)

    Yang, Ting; Xu, Feifei; Fang, Danjun; Chen, Yun

    2015-11-17

    The distinct roles of protein isoforms in cancer are becoming increasingly evident. FRα and FRβ, two major isoforms of the folate receptor family, generally have different cellular distribution and tissue specificity. However, the presence of FRβ in breast tumors, where FRα is normally expressed, complicates this situation. Prior to applying any FR isoform-based diagnosis and therapeutics, it is essential to monitor the expression profile of FR isoforms in a more accurate manner. An LC-MS/MS-based targeted proteomics assay was developed and validated in this study because of the lack of suitable methodology for the simultaneous and specific measurement of highly homologous isoforms occurring at low concentrations. FRα and FRβ monitoring was achieved by measuring their surrogate isoform-specific peptides. Five human breast cell lines, isolated macrophages and 60 matched pairs of breast tissue samples were subjected to the analysis. The results indicated that FRβ was overexpressed in tumor-associated macrophages (TAMs) but not epithelial cells, in addition to an enhanced level of FRα in breast cancer cells and tissue samples. Moreover, the levels of the FR isoforms were evaluated according to the histology, histopathological features and molecular subtypes of breast cancer. Several positive associations with PR/ER and HER2 status and metastasis were revealed.

  1. Le corps des anges

    OpenAIRE

    Margel,Serge

    2017-01-01

    Ce séminaire fait suite à un cycle de conférences données en 2014 sur la notion de corps de chair dans le premier christianisme. Cette année la recherche a porté sur le corps spécifique des anges dans la patristique grecque et latine. Sur la base d’un corpus de textes délimité, j’ai essayé de montrer deux choses principales : d’un côté, on ne peut pas comprendre la spécificité du corps des anges sans l’inscrire dans le champ de l’angélologie chrétienne, qui assimile l’ange et l’âme, et d’un a...

  2. Typologie des Accidents Cyclistes

    OpenAIRE

    Amoros, Emmanuelle; BILLOT-GRASSET, Alice; Hours, Martine

    2015-01-01

    L'usage du vélo est en hausse en ville ; cette pratique est encouragée dans le cadre du développement durable et de la lutte contre la sédentarité. Pour accompagner cela, il faut réduire les risques d'accident, et pour ce faire, mieux les connaître. Nous utilisons le Registre des victimes de la circulation routière du Rhône, basé sur les services hospitaliers (dont les urgences) ; il est quasi-exhaustif : env. 1100 blessés à vélo/an versus 120 dans les données officielles. L'ensemble des cycl...

  3. Table des tableaux

    OpenAIRE

    2014-01-01

    Présentation schématique de la production et de la transformation de l’acier 16 Chronologie des nationalisations en France 33 Importations de coke de la France 48 Comparaison entre les prix de l’acier en Allemagne et en France, août 1949 99 Prix du marché intérieur et du marché mondial pour les laminés marchands (qualité Thomas) 101 Évolution de la production allemande et française d’acier brut 119 Comparaison des coûts de l’extraction de charbon en Allemagne et en France, 1949 138 Évolution ...

  4. Table des illustrations

    OpenAIRE

    2017-01-01

    Illustration de couverture : Dolmen n° 2 des Géandes avec le Mont Ventoux en arrière-plan, octobre 2006 (cliché Wolfgang Pape). Illustration de la quatrième de couverture : René Gilles sur le dolmen n° 5 des Géandes en mars 1989 (cliché Wolfgang Pape). Fig. 1 - Gorges de l’Ardèche au niveau de la grotte de Saint-Marcel (cliché Bernard Gély) 21 Fig. 2 - Doline de Bidon (cliché Wolfgang Pape) 21 Fig. 3 - Paysage de garrigue (cliché Wolfgang Pape) 22 Fig. 4 - Données radio-chronométriques de l’A...

  5. Direction des Publications

    African Journals Online (AJOL)

    Synthese

    accroissement des pressions sur les écosystèmes et les espèces qu‟ils renferment. La pollution de ... Exacerbée par le changement climatique, cette anthropisation .... majeure partie de son aire de répartition. .... de cette région. .... savoir les impacts à plus grande échelle et ..... habitats et la totalité de l'aire géographique.

  6. Le vernis des apparences

    Directory of Open Access Journals (Sweden)

    Grazia Nicosia

    2010-04-01

    Full Text Available Le but de cet article est de comprendre l’incidence sémiotique du nettoyage d’un tableau ainsi que la résultante cognitive induite sur l’observateur. Cette étude confronte l’analyse des phénomènes visuels engendrés par le jaunissement du vernis et son retrait, à des entretiens réalisés à dessein. Le retrait d’un vernis jauni change considérablement l’image. L’heure, le climat, la saison et l’activité même des personnages s’en trouvent ainsi modifié. La patine peut être perçue, soit comme un obstacle à l’exploration, soit pour ceux qui l’apprécient, comme le medium d’une relation plus intime entre l’œuvre et l’observateur.The purpose of this article is to understand the semiotic impact of the cleaning of a painting and the cognitive effects induced on the observer. This study analyses conjointly the visual phenomenon generated by yellowing varnish and its removal and controlled interviews of observers.The removal of yellowing varnish modifies considerably the perception of the picture. Daytime, climate, season and characters activities are modified. The patina may be perceived either as an obstacle to the painting exploration, or, for those who appreciate it, as a medium for a closer relationship between the painting and the observer.

  7. Dictionnaire des risques psychosociaux

    CERN Document Server

    Zawieja, Philippe

    2014-01-01

    Stress, suicide, harcèlement, épuisement professionnel, workaholism... Au-delà de la souffrance qu'elles désignent, ces notions souvent récentes constituent une approche inédite, et demandent à mieux être comprises, dans leur ensemble et isolément. C'est tout l'enjeu de ce dictionnaire, pionnier en son genre. Le lecteur y trouvera représentés, avec les 314 entrées (rédigées par 251 contributeurs) qui le composent, tous les champs disciplinaires s'intéressant à la souffrance au travail : psychologie du travail et des organisations, psychologie sociale et psychosociologie, psychanalyse, psychopathologie et psychiatrie, ergonomie, sociologie du travail et des organisations, médecine du travail, droit du travail et de la sécurité sociale, sciences de gestion, philosophie... Y sont détaillés les principaux concepts, notions, approches, méthodes, théories, outils, études, etc., ayant cours dans l'étude des risques psychosociaux, mais aussi certaines professions emblématiques (infirmières,...

  8. Grundlagen des Tissue Engineering

    Science.gov (United States)

    Mayer, Jörg; Blum, Janaki; Wintermantel, Erich

    Die Organtransplantation stellt eine verbreitete Therapie dar, um bei krankheitsoder unfallbedingter Schädigung eines Organs die Gesamtheit seiner Funktionen wieder herzustellen, indem es durch ein Spenderorgan ersetzt wird. Organtransplantationen werden für die Leber, die Niere, die Lunge, das Herz oder bei schweren grossflächigen Verbrennungen der Haut vorgenommen. Der grosse apparative, personelle und logistische Aufwand und die Risiken der Transplantationschirurgie (Abstossungsreaktionen) sowie die mangelnde Verfügbarkeit von immunologisch kompatiblen Spenderorganen führen jedoch dazu, dass der Bedarf an Organtransplantaten nur zu einem sehr geringen Teil gedeckt werden kann. Sind Spenderorgane nicht verfügbar, können in einzelnen Fällen lebenswichtige Teilfunktionen, wie beispielsweise die Filtrationsfunktion der Niere durch die Blutreinigung mittels Dialyse ersetzt oder, bei mangelnder Funktion der Bauchspeicheldrüse (Diabetes), durch die Verabreichung von Insulin ein normaler Zustand des Gesamtorganismus auch über Jahre hinweg erhalten werden. Bei der notwendigen lebenslangen Anwendung apparativer oder medikamentöser Therapie können für den Patienten jedoch häufig schwerwiegende, möglicherweise lebensverkürzende Nebenwirkungen entstehen. Daher werden in der Forschung Alternativen gesucht, um die Funktionen des ausgefallenen Organs durch die Implantation von Zellen oder in vitro gezüchteten Geweben möglichst umfassend wieder herzustellen. Dies erfordert biologisch aktive Implantate, welche die für den Stoffwechsel des Organs wichtigen Zellen enthalten und einen organtypischen Stoffwechsel entfalten.

  9. Isoform-specific targeting of ROCK proteins in immune cells

    OpenAIRE

    Zanin-Zhorov, Alexandra; Flynn, Ryan; Waksal, Samuel D.; Blazar, Bruce R.

    2016-01-01

    ABSTRACT Rho-associated kinase 1 (ROCK1) and ROCK2 are activated by Rho GTPase and control cytoskeleton rearrangement through modulating the phosphorylation of their down-stream effector molecules. Although these 2 isoforms share more than 90% homology within their kinase domain the question of whether ROCK proteins function identically in different cell types is not clear. By using both pharmacological inhibition and genetic knockdown approaches recent studies suggest that the ROCK2 isoform ...

  10. Neuronal profilin isoforms are addressed by different signalling pathways.

    Directory of Open Access Journals (Sweden)

    Kai Murk

    Full Text Available Profilins are prominent regulators of actin dynamics. While most mammalian cells express only one profilin, two isoforms, PFN1 and PFN2a are present in the CNS. To challenge the hypothesis that the expression of two profilin isoforms is linked to the complex shape of neurons and to the activity-dependent structural plasticity, we analysed how PFN1 and PFN2a respond to changes of neuronal activity. Simultaneous labelling of rodent embryonic neurons with isoform-specific monoclonal antibodies revealed both isoforms in the same synapse. Immunoelectron microscopy on brain sections demonstrated both profilins in synapses of the mature rodent cortex, hippocampus and cerebellum. Both isoforms were significantly more abundant in postsynaptic than in presynaptic structures. Immunofluorescence showed PFN2a associated with gephyrin clusters of the postsynaptic active zone in inhibitory synapses of embryonic neurons. When cultures were stimulated in order to change their activity level, active synapses that were identified by the uptake of synaptotagmin antibodies, displayed significantly higher amounts of both isoforms than non-stimulated controls. Specific inhibition of NMDA receptors by the antagonist APV in cultured rat hippocampal neurons resulted in a decrease of PFN2a but left PFN1 unaffected. Stimulation by the brain derived neurotrophic factor (BDNF, on the other hand, led to a significant increase in both synaptic PFN1 and PFN2a. Analogous results were obtained for neuronal nuclei: both isoforms were localized in the same nucleus, and their levels rose significantly in response to KCl stimulation, whereas BDNF caused here a higher increase in PFN1 than in PFN2a. Our results strongly support the notion of an isoform specific role for profilins as regulators of actin dynamics in different signalling pathways, in excitatory as well as in inhibitory synapses. Furthermore, they suggest a functional role for both profilins in neuronal nuclei.

  11. Cell-specific expression of TLR9 isoforms in inflammation.

    Science.gov (United States)

    McKelvey, Kelly J; Highton, John; Hessian, Paul A

    2011-02-01

    Toll-like receptors (TLRs) are key pattern recognition receptors during an immune response. With five isoforms of human TLR9 described, we hypothesised that differential expression of TLR9 isoforms in different cell types would result in variable contributions to the overall input from TLR9 during inflammation. We assessed the molecular expression of the TLR9 isoforms, TLR9-A, -C and -D. In normal peripheral blood mononuclear cells, B-lymphocytes express ∼100-fold more TLR9-A transcript than monocytes or T-lymphocytes, which predominantly express the TLR9-C transcript. Switches in isoform predominance accompany B-lymphocyte development. TLR9 protein expression in rheumatoid inflammatory lesions reflected the TLR9 isoform expression by immune cells. Herein we suggest that B-lymphocytes and plasmacytoid dendritic cells contribute the ∼3-fold higher TLR9-A transcript levels observed in inflamed synovium when compared to subcutaneous rheumatoid nodules. In contrast, macrophages and T-lymphocytes contribute the ∼4-fold higher TLR9-C transcript levels seen in nodules, compared to synovia. From protein sequence, predictions of subcellular localisation suggest TLR9-B may locate to the mitochondria, whereas TLR9-D adopts an opposing orientation in the endoplasmic reticulum. Consistent with this, structure models raise the possibility of alternative ligands for the TLR9-B and TLR9-D variants. Our results highlight differences in the expression of human TLR9 isoforms in normal and inflamed tissues, with differing contributions to inflammation.

  12. Yeast ADP/ATP Carrier Isoform 2

    Science.gov (United States)

    Clémençon, Benjamin; Rey, Martial; Trézéguet, Véronique; Forest, Eric; Pelosi, Ludovic

    2011-01-01

    The mitochondrial ADP/ATP carrier, or Ancp, is a member of the mitochondrial carrier family responsible for exchanging ADP and ATP across the mitochondrial inner membrane. ADP/ATP transport involves Ancp switching between two conformational states. These can be analyzed using specific inhibitors, carboxyatractyloside (CATR) and bongkrekic acid (BA). The high resolution three-dimensional structure of bovine Anc1p (bAnc1p), as a CATR-carrier complex, has been solved. However, because the structure of the BA-carrier complex has not yet been determined, the detailed mechanism of transport remains unknown. Recently, sample processing for hydrogen/deuterium exchange experiments coupled to mass spectrometry was improved, providing novel insights into bAnc1p conformational transitions due to inhibitor binding. In this work we performed both hydrogen/deuterium exchange-mass spectrometry experiments and genetic manipulations. Because these are very difficult to apply with bovine Anc1p, we used Saccharomyces cerevisiae Anc isoform 2 (ScAnc2p). Significant differences in solvent accessibility were observed throughout the amino acid sequence for ScAnc2p complexed to either CATR or BA. Interestingly, in detergent solution, the conformational dynamics of ScAnc2p were dissimilar to those of bAnc1p, in particular for the upper half of the cavity, toward the intermembrane space, and the m2 loop, which is thought to be easily accessible to the solvent from the matrix in bAnc1p. Our study then focused on the methionyl residues of the Ancp signature sequence, RRRMMM. All our results indicate that the methionine cluster is involved in the ADP/ATP transport mechanism and confirm that the Ancp cavity is a highly dynamic structure. PMID:21868387

  13. Reticulation des fibres lignocellulosiques

    Science.gov (United States)

    Landrevy, Christel

    Pour faire face à la crise économique la conception de papier à valeur ajoutée est développée par les industries papetières. Le but de se projet est l'amélioration des techniques actuelles de réticulation des fibres lignocellulosiques de la pâte à papier visant à produire un papier plus résistant. En effet, lors des réactions de réticulation traditionnelles, de nombreuses liaisons intra-fibres se forment ce qui affecte négativement l'amélioration anticipée des propriétés physiques du papier ou du matériau produit. Pour éviter la formation de ces liaisons intra-fibres, un greffage sur les fibres de groupements ne pouvant pas réagir entre eux est nécessaire. La réticulation des fibres par une réaction de « click chemistry » appelée cycloaddition de Huisgen entre un azide et un alcyne vrai, catalysée par du cuivre (CuAAC) a été l'une des solutions trouvée pour remédier à ce problème. De plus, une adaptation de cette réaction en milieux aqueux pourrait favoriser son utilisation en milieu industriel. L'étude que nous désirons entreprendre lors de ce projet vise à optimiser la réaction de CuAAC et les réactions intermédiaires (propargylation, tosylation et azidation) sur la pâte kraft, en milieu aqueux. Pour cela, les réactions ont été adaptées en milieu aqueux sur la cellulose microcristalline afin de vérifier sa faisabilité, puis transférée à la pâte kraft et l'influence de différents paramètres comme le temps de réaction ou la quantité de réactifs utilisée a été étudiée. Dans un second temps, une étude des différentes propriétés conférées au papier par les réactions a été réalisée à partir d'une série de tests papetiers optiques et physiques. Mots Clés Click chemistry, Huisgen, CuAAC, propargylation, tosylation, azidation, cellulose, pâte kraft, milieu aqueux, papier.

  14. Recombinant erythropoietin in humans has a prolonged effect on circulating erythropoietin isoform distribution

    DEFF Research Database (Denmark)

    Aachmann-Andersen, Niels Jacob; Just Christensen, Søren; Lisbjerg, Kristian

    2014-01-01

    The membrane-assisted isoform immunoassay (MAIIA) quantitates erythropoietin (EPO) isoforms as percentages of migrated isoforms (PMI). We evaluated the effect of recombinant human EPO (rhEPO) on the distribution of EPO isoforms in plasma in a randomized, placebo-controlled, double-blinded, cross...

  15. Sistema de servocontrol visual empleando redes neuronales y filtros en el dominio de CIELAB//Visual servo-control system using neural networks and filters based on CIELAB

    Directory of Open Access Journals (Sweden)

    Germán Buitrago Salazar

    2015-05-01

    Full Text Available En este trabajo se presentan los resultados de un sistema servocontrol visual de un brazo robótico de seis grados de libertad. Para esto, se utiliza una red neuronal de tipo feed forward, entrenada por back propagation, para determinar la distancia entre el brazo robótico y un objeto de referencia, que permite ubicarlo en un espacio de trabajo. Las entradas de la red corresponden a la información obtenida de las imágenes capturadas por el Kinect, utilizando un filtro que discrimina la posición de los elementos, en el espacio de color CIELAB (Commission Internationale de l'Eclairage L*a*b components. El resultado de esta investigación demostró que la distancia estimada por la red tiene un margen de error menor, que el algoritmo propuesto en otros trabajos. Igualmente, se probó que el sistema de procesamiento de imágenes es más robusto a ruidos digitales, en comparación con los sistemas que utilizan filtros en el dominio RGB (Red-Green-Blue.Palabras claves: sistema de servocontrol visual, CIELAB, redes neuronales, filtrado de imágenes.______________________________________________________________________________AbstractIn this paper the results of visual servo-control system for a robotic arm with six degrees of freedom are presented. For this purpose, a feed fordward neural network, which was trained by back propagation, is used to determine the distance between the robot arm and a reference object and sitting the robot in the workspace. The inputs of neural network correspond to the information obtained from the images captured by the Kinect, using a filter that discriminates the position of the elements in the CIELAB (Commission Internationale de l'Eclairage L*a*bcomponents color space. The result of this research showed that the estimated distance with the network has an errorless than the algorithm proposed in other works. Similarly, it was proved that the image processing system is more robust to digital noise, compared to

  16. MODELACIÓN DE LA ESTRUCTURA JERÁRQUICA DE MACROINVERTEBRADOS BENTÓNICOS A TRAVÉS DE REDES NEURONALES ARTIFICIALES

    Directory of Open Access Journals (Sweden)

    CLAUDIA RICO

    2009-01-01

    Full Text Available El estudio de la estructura jerárquica de comunidades ecológicas, se ha sintetizado de manera regular a través de técnicas multivariadas de ordenación o clasificación. Sin embargo, al contarse actualmente con herramientas analíticas de computación bioinspirada provenientes de la inteligencia artificial, existe la oportunidad de establecer modelos ecológicos, con características deseables como flexibilidad, exactitud, robustez y confiabilidad. En este contexto, esta investigación utilizó dos métodos computacionales de utilidad en ecoinformática, referidos a redes neuronales artificiales (RNARs para la modelación de la estructura jerárquica de una comunidad de macroinvertebrados bentónicos en términos de auto-organización y predicción. El primer método de modelación consistió en un mapa de auto-organización (MAU, una herramienta de aprendizaje no supervisado que clasificó las especies de macroinvertebrados; este MAU tomó en la capa de entrada la abundancia de cada taxa, y en la de salida proyectó su clasificación en 15 unidades y cuatro agrupamientos jerárquicos. La segunda RNA, correspondió a un Perceptrón multicapa de alimentación adelantada con algoritmo de retropropagación, que modeló separadamente la riqueza y la abundancia de Ephemeroptera, Coleoptera y Trichoptera (ECT, en función de nueve variables fisicoquímicas; la arquitectura del perceptrón correspondió a una constitución de nueve, siete, y una neurona en las capas de entrada, intermedia y salida, respectivamente. Los resultados sugieren que las RNARs utilizadas evidenciaron tanto los patrones jerárquicos, como los de riqueza y abundancia de ECT de manera adecuada, al tiempo que facilitaron el análisis de los datos y el entendimiento de la dinámica de la comunidad de macroinvertebrados, objeto de estudio.

  17. MODELACIÓN DE LA ESTRUCTURA JERÁRQUICA DE MACROINVERTEBRADOS BENTÓNICOS A TRAVÉS DE REDES NEURONALES ARTIFICIALES

    Directory of Open Access Journals (Sweden)

    Fernandez Nelson

    2009-12-01

    Full Text Available El estudio de la estructura jerárquica de comunidades ecológicas, se ha sintetizado de manera regular a través de técnicas multivariadas de ordenación o clasificación. Sin embargo, al contarse actualmente con herramientas analíticas de computación bioinspirada provenientes de la inteligencia artificial, existe la oportunidad de establecer modelos ecológicos, con características deseables como flexibilidad, exactitud, robustez y confiabilidad. En este contexto, esta investigación utilizó dos métodos computacionales de utilidad en ecoinformática, referidos a redes neuronales artificiales (RNARs para la modelación de la estructura jerárquica de una comunidad de macroinvertebrados bentónicos en términos de auto-organización y predicción. El primer método de modelación consistió en un mapa de auto-organización (MAU, una herramienta de aprendizaje no supervisado que clasificó las especies de macroinvertebrados; este MAU tomó en la capa de entrada la abundancia de cada taxa, y en la de salida proyectó su clasificación en 15 unidades y cuatro agrupamientos jerárquicos. La segunda RNA, correspondió a un Perceptrón multicapa de alimentación adelantada con algoritmo de retropropagación, que modeló separadamente la riqueza y la abundancia de Ephemeroptera, Coleoptera y Trichoptera (ECT, en función de nueve variables fisicoquímicas; la arquitectura del perceptrón correspondió a una constitución de nueve, siete, y una neurona en las capas de entrada, intermedia y salida, respectivamente. Los resultados sugieren que las RNARs utilizadas evidenciaron tanto los patrones jerárquicos, como los de riqueza y abundancia de ECT de manera adecuada, al tiempo que facilitaron el análisis de los datos y el entendimiento de la dinámica de la comunidad de macroinvertebrados, objeto de estudio.

  18. Structural diversity and evolution of the N-terminal isoform-specific region of ecdysone receptor-A and -B1 isoforms in insects

    Directory of Open Access Journals (Sweden)

    Kubo Takeo

    2010-02-01

    Full Text Available Abstract Background The ecdysone receptor (EcR regulates various cellular responses to ecdysteroids during insect development. Insects have multiple EcR isoforms with different N-terminal A/B domains that contain the isoform-specific activation function (AF-1 region. Although distinct physiologic functions of the EcR isoforms have been characterized in higher holometabolous insects, they remain unclear in basal direct-developing insects, in which only A isoform has been identified. To examine the structural basis of the EcR isoform-specific AF-1 regions, we performed a comprehensive structural comparison of the isoform-specific region of the EcR-A and -B1 isoforms in insects. Results The EcR isoforms were newly identified in 51 species of insects and non-insect arthropods, including direct-developing ametabolous and hemimetabolous insects. The comprehensive structural comparison revealed that the isoform-specific region of each EcR isoform contained evolutionally conserved microdomain structures and insect subgroup-specific structural modifications. The A isoform-specific region generally contained four conserved microdomains, including the SUMOylation motif and the nuclear localization signal, whereas the B1 isoform-specific region contained three conserved microdomains, including an acidic activator domain-like motif. In addition, the EcR-B1 isoform of holometabolous insects had a novel microdomain at the N-terminal end. Conclusions Given that the nuclear receptor AF-1 is involved in cofactor recruitment and transcriptional regulation, the microdomain structures identified in the isoform-specific A/B domains might function as signature motifs and/or as targets for cofactor proteins that play essential roles in the EcR isoform-specific AF-1 regions. Moreover, the novel microdomain in the isoform-specific region of the holometabolous insect EcR-B1 isoform suggests that the holometabolous insect EcR-B1 acquired additional transcriptional

  19. Etude des cas: Gestion alternatîves des conflits

    OpenAIRE

    Lo, H.

    2006-01-01

    Metadata only record Pour mieux consolider les acquis consensuels pour la gestion alternative des conflits, il nous paraît essentiel de maîtriser un certain nombre de facteurs ayant trait au "partage" des gommeraies entre autres: Available in SANREM office, FS

  20. Multiple isoform recovery (MIR)-PCR: a simple method for the isolation of related mRNA isoforms.

    OpenAIRE

    Fagotti, A; Gabbiani, G.; Pascolini, R; Neuville, P

    1998-01-01

    We present a rapid and efficient method for the detection of related transcripts with different expression levels. This approach combines the rapid amplification of cDNA ends (RACE) method with a cDNA subtractive technique. The strategy is based on successive subtractions of prevalent isoforms resulting in enrichment of less expressed transcripts. For each subtraction, a biotinylated primer specific for the prevalent isoform is hybridized on the total cDNA and the hybrid is retained on a stre...

  1. Analysis and modelling of the use of a nickel/metal hydride battery in the Autarkic Hybrid; Analyse und Modellierung des Einsatzes einer Nickel/Metallhydrid-Batterie im Autarken Hybrid

    Energy Technology Data Exchange (ETDEWEB)

    Angloher, J.; Wagner, U.

    1999-07-01

    The optimal operation of traction batteries in hybrid drive concepts requires a battery analysis and modelling in order to prepare the vehicle integration of the battery. In order to use a Ni/MH battery in the Autarkic Hybrid a battery management was set-up in the frame of the special research field 365, whose development depends on the measuring-technical analysis of the energetic battery performance and on a new method to determine the load condition. The contribution describes how neuronal networks can be used by extending conventional methods in order to better determine the actual load condition of the battery. (orig.) [German] Der optimale Betrieb von Traktionsbatterien in hybriden Antriebskonzepten erfordert die vorherige Batterieanalyse und -modellierung, um die Fahrzeugintegration der Batterie vorzubereiten. Fuer den Einsatz einer Ni/MH-Batterie im Autarken Hybrid wurde im Rahmen des Sonderforschungsbereichs 365 ein Batteriemanagement aufgebaut, dessen Entwicklung auf der messtechnischen Analyse des energetischen Batterieverhaltens und einem neuen Verfahren der Ladezustandsbestimmung basiert. Der Beitrag beschreibt, wie in Erweiterung herkoemmlicher Methoden neuronale Netze verwendet werden koennen, um den aktuellen Ladezustand der Batterie praeziser zu bestimmen. (orig.)

  2. Missile Aerodynamics (Aerodynamique des Missiles)

    Science.gov (United States)

    1998-11-01

    guerre froide la production des missiles a baisse’, avec pour consequence une diminution des budgets de d6veloppement. Les nouveaux types de conflits ...Roma) Directeur - Gestion de l’information LUXEMBOURG (Recherche et developpement) - DRDGI 3 Voir Belgique Ministbre de la Difense nationale NORVEGE

  3. Distinct functional interactions between actin isoforms and nonsarcomeric myosins.

    Directory of Open Access Journals (Sweden)

    Mirco Müller

    Full Text Available Despite their near sequence identity, actin isoforms cannot completely replace each other in vivo and show marked differences in their tissue-specific and subcellular localization. Little is known about isoform-specific differences in their interactions with myosin motors and other actin-binding proteins. Mammalian cytoplasmic β- and γ-actin interact with nonsarcomeric conventional myosins such as the members of the nonmuscle myosin-2 family and myosin-7A. These interactions support a wide range of cellular processes including cytokinesis, maintenance of cell polarity, cell adhesion, migration, and mechano-electrical transduction. To elucidate differences in the ability of isoactins to bind and stimulate the enzymatic activity of individual myosin isoforms, we characterized the interactions of human skeletal muscle α-actin, cytoplasmic β-actin, and cytoplasmic γ-actin with human myosin-7A and nonmuscle myosins-2A, -2B and -2C1. In the case of nonmuscle myosins-2A and -2B, the interaction with either cytoplasmic actin isoform results in 4-fold greater stimulation of myosin ATPase activity than was observed in the presence of α-skeletal muscle actin. Nonmuscle myosin-2C1 is most potently activated by β-actin and myosin-7A by γ-actin. Our results indicate that β- and γ-actin isoforms contribute to the modulation of nonmuscle myosin-2 and myosin-7A activity and thereby to the spatial and temporal regulation of cytoskeletal dynamics. FRET-based analyses show efficient copolymerization abilities for the actin isoforms in vitro. Experiments with hybrid actin filaments show that the extent of actomyosin coupling efficiency can be regulated by the isoform composition of actin filaments.

  4. Differential activities of glucocorticoid-induced leucine zipper protein isoforms.

    Science.gov (United States)

    Soundararajan, Rama; Wang, Jian; Melters, Daniël; Pearce, David

    2007-12-14

    Glucocorticoid-induced leucine zipper protein (GILZ) is expressed in both epithelial and immune tissues and modulates a variety of cellular functions, including proliferation and epithelial sodium channel (ENaC) activity. A number of reports have described various GILZ activities, focusing on a single isoform with molecular mass of approximately 17 kDa, now termed GILZ1. In GILZ immunoblots using a newly developed antiserum, we detected multiple species in extracts from cultured kidney cells. Mass spectrometric analysis revealed that one of these represented a previously uncharacterized distinct isoform of GILZ, GILZ2. Rapid amplification of cDNA ends was used to clone cDNAs corresponding to four isoforms, which, in addition to GILZ1 and GILZ2, included new isoforms GILZ3 and GILZ4. Heterologous expression of these four GILZ isoforms in cultured cells revealed striking functional differences. Notably, GILZ1 was the only isoform that significantly stimulated ENaC-mediated Na+ current in a kidney collecting duct cell line, although GILZ2 and GILZ3 also stimulated ENaC surface expression in HEK 293 cells. GILZ1 and GILZ3, and to a lesser extent GILZ2, inhibited ERK phosphorylation. Interestingly, GILZ4, which had no effect on either ENaC or ERK, potently suppressed cellular proliferation, as did GILZ1, but not GILZ2 or GILZ3. Finally, rat and mouse tissues all expressed multiple GILZ species but varied in the relative abundance of each. These data suggest that multiple GILZ isoforms are expressed in most cells and tissues and that these play distinct roles in regulating key cellular functions, including proliferation and ion transport. Furthermore, GILZ inhibition of ERK appears to play an essential role in stimulation of cell surface ENaC but not in inhibition of proliferation.

  5. Therapie des Status epilepticus

    Directory of Open Access Journals (Sweden)

    Trinka E

    2009-01-01

    Full Text Available Der Status epilepticus (SE ist nach dem Schlaganfall der häufigste neurologische Notfall. Er stellt als generaliserter konvulsiver SE (GKSE die schwerste Ausprägung eines epileptischen Anfalls dar, der mit einer signifikanten Morbidität und einer ca. 20%igen Letalität verbunden ist. Nur bei der Hälfte der Patienten mit SE besteht zuvor eine Epilepsie; die meisten Fälle sind symptomatisch, wobei Schädel- Hirn-Traumata, zerebrovaskuläre Erkrankungen, ZNS-Infektionen und metabolische Ursachen führend sind. Bei Patienten mit vorbestehender Epilepsie lässt sich eine frühe Phase des GKSE erkennen, in der die Anfälle crescendoartig zunehmen, bis sie in kontinuierliche Anfallsaktivität münden (etablierte Phase. Das Management eines GKSE verlangt rasches und beherztes Vorgehen. Neben der sofort einzuleitenden Therapie muss gleichzeitig die artdiagnostische Zuordnung des SE und die Ursache erkannt und behandelt werden. Als Therapie der ersten Wahl sind Benzodiazepine etabliert, wobei intravenösem (i.v. Lorazepam gegenüber Diazepam der Vorzug zu geben ist. Versagt die Therapie mit Benzodiazepinen, muss rasch und konsequent nach einem Stufenschema vorgegangen werden. Phenytoin/Fosphenytoin, Valproinsäure, Levetiracetam und Lacosamid sind als i.v. Formulierung erhältliche Antiepileptika. Obwohl Vergleichsstudien hier fehlen, wird Phenytoin bevorzugt. Valproat und Levetiracetam sind nicht sedierend und kardiovaskulär gut verträglich, sodass hier Alternativen zu Phenytoin vorhanden sind. Lacosamid ist eine neue Therapieoption, deren Stellenwert erst bestimmt werden muss. Versagt auch die zweite Therapiestufe, so muss der Patient intubiert und in Allgemeinanästhesie intensivmedizinisch behandelt werden. Dafür stehen Thiopental, Propofol oder hoch dosiertes Midazolam zu Verfügung. Durch den eklatanten Mangel an randomisierten Studien bleibt die Therapie des GKSE empirisch und durch den Off-label-Einsatz gekennzeichnet.

  6. Mécanique des fluides

    CERN Document Server

    Cengel, Y A

    2017-01-01

    La mécanique des fluides est un outil performant qui permet d'expliquer les phénomènes qui nous entourent de l'échelle microscopique à l'échelle macroscopique. Elle est aussi à la base du développement de nombreuses technologies. Cet ouvrage à destination des étudiants donne une vision complète de la mécanique des fluides. Bien que la mécanique des fluides puisse souvent paraître rébarbative aux yeux des étudiants, cet ouvrage valorise ce domaine d'enseignement en l'illustrant de nombreux exemples issus de l'ingénierie navale, l'aéronautique, la météorologie, etc.

  7. Uso de redes neuronales artificiales en predicción de morfología mandibular a través de variables craneomaxilares en una vista posteroanterior / Use of Artificial Neural Networks for Mandibular Morphology Prediction through Craniomaxillar Variables...

    OpenAIRE

    Niño Sandoval, Tania Camila; Universidad Nacional de Colombia, Bogotá; Guevara Pérez, Sonia Victoria; Universidad Nacional de Colombia, Bogotá; González, Fabio Augusto; Universidad Nacional de Colombia, Bogotá; Jaque, Robinson Andrés; Universidad Nacional de Colombia, Bogotá; Infante Contreras, Clementina; Universidad Nacional de Colombia, Bogotá

    2016-01-01

    RESUMEN. Antecedentes: la predicción de la morfología mandibular es importante tanto en la reconstrucción facial con fines forenses, como en ortodoncia y cirugía maxilofacial. Dicho proceso se ha realizado a través de métodos paramétricos y lineales basándose en poblaciones caucásicas; asimismo, dichos análisis se realizan en radiografías de perfil más no se tiene en cuenta una predicción mandibular desde una vista posteroanterior. Propósito: predecir a través de redes neuronales artificiales...

  8. Elaboración de un modelo evolutivo híbrido de algoritmos genéticos y redes neuronales artificiales para dosificaciones de mezclas de concreto reforzadas con fibras metálicas

    Directory of Open Access Journals (Sweden)

    Luis Octavio González Salcedo

    2016-06-01

    Full Text Available En un ambiente computacional, un modelo evolutivo es desarrollado para proponer dosificaciones de mezclas de concretos reforzados con fibras metálicas, para solicitudes de resistencia a la compresión. El modelo es híbrido al incluir un sistema de dosificación basado en algoritmos genéticos y un sistema de estimación de propiedades basado en redes neuronales artificiales. Los resultados obtenidos son comparados con un sistema de dosificaciones reportado experimentalmente. Estas comparaciones muestran una aproximación en el proceso de simulación. Además, por las características del modelo, se considera de aporte en la tecnología del concreto.

  9. ¿Cómo estás atención? Cuáles son y cómo se evalúan las redes neuronales de la atención

    Directory of Open Access Journals (Sweden)

    Fernando Gabriel Luna

    2016-01-01

    Full Text Available Nuestro sistema cognitivo cuenta con redes neuronales que desarrollan diferentes funciones atencionales para afrontar la complejidad estimular del medio ambiente y adaptar nuestro comportamiento a la naturaleza y exigencia de múltiples situaciones. Las redes de la atención conforman un sistema de estructuras cerebrales específicas conectadas y sincronizadas entre sí que nos permiten orientarnos, alertarnos ante algunas señales, sostener nuestra atención, y seleccionar y controlar ciertos estímulos. A continuación se describe la estructura de estas redes, cómo operan en diversas situaciones, y cómo es posible obtener una medida confiable del rendimiento de cada una de las funciones atencionales.

  10. Molekulare Formen des PSA in der Diagnostik des Prostatakarzinoms

    Directory of Open Access Journals (Sweden)

    Lein M

    2003-01-01

    Full Text Available Das prostataspezifische Antigen ist die wichtigste Kenngröße in der Diagnostik und Therapieüberwachung des Prostatakarzinoms. Zur besseren Abgrenzung zwischen Männern mit und ohne Prostatakarzinom, aber auch zur Früherkennung, haben sich die molekularen Formen des PSA als wertvolle Entscheidungshilfen erwiesen. Es wird eine Übersicht über bisher vorliegende Informationen zur Aussagekraft des freien PSA, des an alpha 1-Antichymotrypsin gebundenen PSA und des komplexierten PSA (cPSA zusammen mit eigenen Resultaten gegeben. Der Quotient aus freiem und Gesamt-PSA hat sich als eine wichtige Entscheidungsgröße in der urologischen Praxis etabliert, mit der Sensitivität und Spezifität der Prostatakarzinomdiagnostik verbessert werden. Die Zahl von Prostatastanzbiopsien im Gesamt-PSA-Bereich von 4–10 ng/ml kann damit reduziert werden. Ein diagnostischer Vorteil der alleinigen Bestimmung des komplexierten PSA bzw. der entsprechenden Quotienten im Vergleich zum Gesamt-PSA und des Quotienten freies PSA zu Gesamt-PSA konnte bisher nicht sicher nachgewiesen werden.

  11. Mécanique des sols et des roches

    CERN Document Server

    Vullier, Laurent; Zhao, Jian

    2016-01-01

    La mécanique des sols et la mécanique des roches sont des disciplines généralement traitées séparément dans la littérature. Pour la première fois, un traité réunit ces deux spécialités, en intégrant également les connaissances en lien avec les écoulements souterrains et les transferts thermiques. A la fois théorique et pratique, cet ouvrage propose tout d'abord une description détaillée de la nature et de la composition des sols et des roches, puis s'attache à la modélisation de problèmes aux conditions limites et présente les essais permettant de caractériser les sols et les roches, tant d'un point de vue mécanique qu'hydraulique et thermique. La problématique des sols non saturés et des écoulements multiphasiques est également abordée. Une attention particulière est portée aux lois de comportement mécanique et à la détermination de leurs paramètres par des essais in situ et en laboratoire, et l'ouvrage offre également une présentation détaillée des systèmes de classi...

  12. Chemical origins of isoform selectivity in histone deacetylase inhibitors.

    Science.gov (United States)

    Butler, Kyle V; Kozikowski, Alan P

    2008-01-01

    Histones undergo extensive posttranslational modifications that affect gene expression. Acetylation is a key histone modification that is primarily regulated by two enzymes, one of which is histone deacetylase (HDAC). The activity of HDAC causes transcriptional silencing of DNA. Eleven distinct zinc-dependent histone deacetylase isoforms have been identified in humans. Each isoform has a unique structure and function, and regulates a unique set of genes. HDAC is responsible for the regulation of many genes involved in cancer cell proliferation, and it has been implicated in the pathogenesis of many neurological conditions. HDAC inhibitors are known to be very effective anti-cancer agents, and research has shown them to be potential treatments for many other conditions. Histone deacetylase inhibitors modify the expression of many genes, and it is possible that inhibition of one isoform could cause epigenetic changes that are beneficial to treatment of a disease, while inhibition of another isoform could cause contradictory changes. Selective HDAC inhibitors will be better able to avoid these types of situations than non-specific inhibitors, and may also be less toxic than pan-HDAC inhibitors. Many potent pan-HDAC inhibitors have already been developed, leaving the development of selective inhibitors at the forefront of HDAC drug development. Certain structural moieties may be added to HDAC inhibitors to give isoform selectivity, and these will be discussed in this review. This review will focus on the applications of selective HDAC inhibitors, inhibitors reported to show selectivity, and the relationship between inhibitor structure and selectivity.

  13. Isoform-targeted regulation of cardiac adenylyl cyclase.

    Science.gov (United States)

    Ishikawa, Yoshihiro

    2003-01-01

    Numerous attempts have been made to develop strategies for regulating the intracellular cyclic AMP signal pharmacologically, with an intention to establish either new medical therapeutic methods or experimental tools. In the past decades, many pharmacological reagents have been identified that regulate this pathway at the level of the receptor. G protein, adenylyl cyclase, cyclic AMP, protein kinase A and phosphodiesterase. Since the cloning of adenylyl cyclase isoforms during the 1990s, investigators including ourselves have tried to find reagents that regulate the activity of this enzyme directly in an isoform-dependent manner. The ultimate goal of developing such reagents would be to regulate the cyclic AMP signal in an organ-dependent manner. Ourselves and other workers have reported that such reagents may vary from a simple cation to kinases. In a more recent study, using the results from crystallographic studies and computer-assisted drug design programs, we have identified subtype-selective regulators of adenylyl cyclase. Such regulators are mostly based upon forskolin, a diterpene compound obtained from Coleus forskolii, that acts directly on adenylyl cyclase to increase the intracellular levels of cyclic AMP. Similarly, novel reagents have been identified that inhibit a specific adenylyl cyclase isoform (e.g. type 5 adenylyl cyclase). Such reagents would potentially provide a new therapeutic strategy to treat hypertension, for example, as well as methods to selectively stimulate or inhibit this adenylyl cyclase isoform, which may be reminiscent of overexpression or knocking out of the cardiac adenylyl cyclase isoform by the use of a pharmacological method.

  14. p53 isoform profiling in glioblastoma and injured brain.

    Science.gov (United States)

    Takahashi, R; Giannini, C; Sarkaria, J N; Schroeder, M; Rogers, J; Mastroeni, D; Scrable, H

    2013-06-27

    The tumor suppressor p53 has been found to be the most commonly mutated gene in human cancers; however, the frequency of p53 mutations varies from 10 to 70% across different cancer types. This variability can partly be explained by inactivating mechanisms aside from direct genomic polymorphisms. The p53 gene encodes 12 isoforms, some of which can modulate full-length p53 activity in cancer. In this study, we characterized p53 isoform expression patterns in glioblastoma, gliosis, non-tumor brain and neural progenitor cells by SDS-PAGE, immunoblot, mass spectrometry and reverse transcription-PCR. We found that the most consistently expressed isoform in glioblastoma, Δ40p53, was uniquely expressed in regenerative processes, such as those involving neural progenitor cells and gliosis compared with tumor samples. Isoform profiling of glioblastoma tissues revealed the presence of both Δ40p53 and full-length p53, neither of which were detected in non-tumor cerebral cortex. Upon xenograft propagation of tumors, p53 levels increased. The variability of overall p53 expression and relative levels of isoforms suggest fluctuations in subpopulations of cells with greater or lesser capacity for proliferation, which can change as the tumor evolves under different growth conditions.

  15. Le silence des agneaux

    Directory of Open Access Journals (Sweden)

    BERNARD ROY

    2012-01-01

    Full Text Available Ce texte est avant tout une réflexion sur la notion d'obéissance, initiée à partir de deux évènements impliquant étroitement des membres de la profession infirmière. L'auteur se réjouit de la prise de parole et de l'implication directe d'infirmières dans le contexte du printemps érable. Il estime que la posture de ces infirmières s'inscrit dans ce que l'éthicien Guy Durand, appelle une obéissance autonome qui peut, du coup, mener à la désobéissance civile, à l'objection de conscience. En prenant exemple sur le silence des infirmières dans le contexte de la fermeture de postes d'infirmières en Minganie, l'auteur estime que cette posture est marginale chez les infirmières qui, majoritairement, adoptent une position de soumission et d'obéissance hétéronome.

  16. La médicalisation des humeurs des enfants

    OpenAIRE

    Monzée, Joël

    2015-01-01

    Les enfants qui ont des comportements dérangeants ou qui souffrent de problèmes neurologiques, comme le trouble déficitaire de l’attention avec ou sans hyperactivité (TDAH), remettent en question nos moyens d’intervention pour les aider. Alors que le contexte européen tend à privilégier une intervention psychoéducative, les milieux nord-américains recourent davantage aux psychostimulants pour réduire les effets des problèmes comportementaux des enfants. Cet article aborde quelques problématiq...

  17. Determining ozone flux in plants using a model for calculating stoma opening width; Bestimmung des Ozonflusses in Pflanzen anhand eines Modells zur Berechnung der Spaltoeffnungsweite

    Energy Technology Data Exchange (ETDEWEB)

    Werner, W.; Bueker, P. [Trier Univ. (Germany). Inst. fuer Geobotanik

    2000-07-01

    To investigate the dose response of clover-leaves to ozone exposure a model of the stoma opening width was developed. It was based on leaf conductance measurements of white clover-clones who were carried out under field-conditions and under defined conditions for the parameters temperature, photosynthetic active radiation and vapour pressure deficit in the laboratory. The field-model predicts the leaf conductance with a smaller coefficient of determination (r{sup 2}=0,61 respectively 0,57) than the lab-model (r{sup 2}=0,98) but is more suitable to estimate potential ozone fluxes. (orig.) [German] Zur Bestimmung des Ozonflusses in Weisskleeblaettern wurde mit Hilfe Kuenstlicher Neuronaler Netze (ANN) ein Modell der Spaltoeffnungsweite entwickelt, das auf Messungen der Blattleitfaehigkeit basiert. Diese Messungen wurden einerseits im Labor unter definierten Bedingungen der Parameter Temperatur, Photosynthetisch Aktive Strahlung und Wasserdampfsaettigungsdefizit der Luft und andererseits unter Freiland-Bedingungen durchgefuehrt. Das Modell aus den Labormessungen sagt mit hohem Bestimmtheitsmass (r{sup 2}=0.98) Blattleitfaehigkeiten vorher, zeigt allerdings Schwaechen in der Uebertragbarkeit auf Freilandmessungen, waehrend das freiland-Modell bei geringerem Bestimmtheismass (r{sup 2}=0,61 bzw. 0,57) realistischere Blattleitfaehigkeit modelliert und sich besser zur Berechnung potentieller Ozonfluesse eignet. (orig.)

  18. Laminin isoforms in endothelial and perivascular basement membranes.

    Science.gov (United States)

    Yousif, Lema F; Di Russo, Jacopo; Sorokin, Lydia

    2013-01-01

    Laminins, one of the major functional components of basement membranes, are found underlying endothelium, and encasing pericytes and smooth muscle cells in the vessel wall. Depending on the type of blood vessel (capillary, venule, postcapillary venule, vein or artery) and their maturation state, both the endothelial and mural cell phenotype vary, with associated changes in laminin isoform expression. Laminins containing the α4 and α5 chains are the major isoforms found in the vessel wall, with the added contribution of laminin α2 in larger vessels. We here summarize current data on the precise localization of these laminin isoforms and their receptors in the different layers of the vessel wall, and their potential contribution to vascular homeostasis.

  19. Proteogenomic Analysis Identifies a Novel Human SHANK3 Isoform

    Directory of Open Access Journals (Sweden)

    Fahad Benthani

    2015-05-01

    Full Text Available Mutations of the SHANK3 gene have been associated with autism spectrum disorder. Individuals harboring different SHANK3 mutations display considerable heterogeneity in their cognitive impairment, likely due to the high SHANK3 transcriptional diversity. In this study, we report a novel interaction between the Mutated in colorectal cancer (MCC protein and a newly identified SHANK3 protein isoform in human colon cancer cells and mouse brain tissue. Hence, our proteogenomic analysis identifies a new human long isoform of the key synaptic protein SHANK3 that was not predicted by the human reference genome. Taken together, our findings describe a potential new role for MCC in neurons, a new human SHANK3 long isoform and, importantly, highlight the use of proteomic data towards the re-annotation of GC-rich genomic regions.

  20. Kryoelektronenmikroskopie von Proteinen: 3D-Struktur (12 A) des Hämocyanins der Schnecke Haliotis

    OpenAIRE

    Meißner, Ulrich

    2000-01-01

    Zusammenfassung:Die Quartärstruktur des respiratorischen Proteins Hämocyanin (Isoform HtH1) aus der marinen Schnecke Haliotis tuberculata wurde vermittels Kryoelektronen-mikroskopie und 3D-Rekonstruktion untersucht. Das Molekül ist zylinderförmig, hat einen Durchmesser von ca. 35 nm und besteht aus einer Zylinderwand und einem internen Kragenkomplex. Dieser wiederum besteht aus einem Collar und einem Arc.Die kryoelektronenmikroskopischen Aufnahmen von in glasartigem Eis fixierten HtH1-Molekül...

  1. Autocrine VEGF isoforms differentially regulate endothelial cell behavior

    Directory of Open Access Journals (Sweden)

    Hideki Yamamoto

    2016-09-01

    Full Text Available Vascular endothelial growth factor A (VEGF is involved in all the essential biology of endothelial cells, from proliferation to vessel function, by mediating intercellular interactions and monolayer integrity. It is expressed as three major alternative spliced variants. In mice, these are VEGF120, VEGF164, and VEGF188, each with different affinities for extracellular matrices and cell surfaces, depending on the inclusion of heparin-binding sites, encoded by exons 6 and 7. To determine the role of each VEGF isoform in endothelial homeostasis, we compared phenotypes of primary endothelial cells isolated from lungs of mice expressing single VEGF isoforms in normoxic and hypoxic conditions. The differential expression and distribution of VEGF isoforms affect endothelial cell functions, such as proliferation, adhesion, migration and integrity, which are dependent on the stability of and affinity to VEGF receptor 2 (VEGFR2. We found a correlation between autocrine VEGF164 and VEGFR2 stability, which is also associated with increased expression of proteins involved in cell adhesion. Endothelial cells expressing only VEGF188, which localizes to extracellular matrices or cell surfaces, presented a mesenchymal morphology and weakened monolayer integrity. Cells expressing only VEGF120 lacked stable VEGFR2 and dysfunctional downstream processes, rendering the cells unviable. Endothelial cells expressing these different isoforms in isolation also had differing rates of apoptosis, proliferation, and signaling via nitric oxide (NO synthesis. These data indicate that autocrine signaling of each VEGF isoform has unique functions on endothelial homeostasis and response to hypoxia, due to both distinct VEGF distribution and VEGFR2 stability, which appears to be, at least partly, affected by differential NO production. This study demonstrates that each autocrine VEGF isoform has a distinct effect on downstream functions, namely VEGFR2-regulated endothelial cell

  2. Identification and characterization of novel NuMA isoforms

    Energy Technology Data Exchange (ETDEWEB)

    Wu, Jin, E-mail: petersdu2112@hotmail.com [Key Laboratory for Cell Proliferation and Regulation of the Ministry of Education, Beijing Normal University, Beijing (China); Xu, Zhe [Department of Clinical Laboratory Diagnosis, Beijing Tiantan Hospital, Capital Medical University, Beijing (China); Core Laboratory for Clinical Medical Research, Beijing Tiantan Hospital, Capital Medical University, Beijing (China); He, Dacheng [Key Laboratory for Cell Proliferation and Regulation of the Ministry of Education, Beijing Normal University, Beijing (China); Lu, Guanting, E-mail: guantlv@126.com [Beijing DnaLead Science and Technology Co., LTD, Beijing (China)

    2014-11-21

    Highlights: • Seven NuMA isoforms generated by alternative splicing were categorized into 3 groups: long, middle and short. • Both exons 15 and 16 in long NuMA were “hotspot” for alternative splicing. • Lower expression of short NuMA was observed in cancer cells compared with nonneoplastic controls. • Distinct localization pattern of short isoforms indicated different function from that of long and middle NuMA. - Abstract: The large nuclear mitotic apparatus (NuMA) has been investigated for over 30 years with functions related to the formation and maintenance of mitotic spindle poles during mitosis. However, the existence and functions of NuMA isoforms generated by alternative splicing remains unclear. In the present work, we show that at least seven NuMA isoforms (categorized into long, middle and short groups) generated by alternative splicing from a common NuMA mRNA precursor were discovered in HeLa cells and these isoforms differ mainly at the carboxyl terminus and the coiled-coil domains. Two “hotspot” exons with molecular mass of 3366-nt and 42-nt tend to be spliced during alternative splicing in long and middle groups. Furthermore, full-length coding sequences of long and middle NuMA obtained by using fusion PCR were constructed into GFP-tagged vector to illustrate their cellular localization. Long NuMA mainly localized in the nucleus with absence from nucleoli during interphase and translocated to the spindle poles in mitosis. Middle NuMA displayed the similar cell cycle-dependent distribution pattern as long NuMA. However, expression of NuMA short isoforms revealed a distinct subcellular localization. Short NuMA were present in the cytosol during the whole cycle, without colocalization with mitotic apparatus. These results have allowed us tentatively to explore a new research direction for NuMA’s various functions.

  3. Habilitations à diriger des recherches

    Directory of Open Access Journals (Sweden)

    2007-04-01

    Full Text Available Pierre Corbin, Avec des dictionnaires pour compagnons.Habilitation à diriger des recherches soutenue le 24 novembre 2006.Composition du juryHenri Béjoint (Lyon 2Franz Josef Hausmann (Erlangen-NürnbergBenoît Habert (Paris 10Pierre Rézeau (CNRSMichel Roché (Toulouse 2Danièle Van de Velde (Lille 3, UMR STL, Directrice d'habilitation.Philippe Sabot, La philosophie, entre pratiques de lecture et pratiques d'écritureHabilitation à diriger des recherches soutenue le 24 novembre 2006.Compositio...

  4. Des cartes dans la classe…

    Directory of Open Access Journals (Sweden)

    R. Gimeno

    1990-09-01

    Full Text Available La majorité des enseignants qui veulent faire des cartes — et les faire réaliser aux élèves — pour répondre aux exigences des instructions officielles, doivent surmonter leur manque de compétences en cartographie et en didactique ainsi que les difficultés propres aux logiciels de cartographie encore peu performants. Ces compétences et la réflexion qui les accompagne sont pourtant accessibles aux enfants de l’école élémentaire…

  5. Wider die Verherrlichung des Weiblichen : Kritik des Ökofeminismus

    OpenAIRE

    Attia, Iman

    1991-01-01

    Die Studie ist eine Kritik am Frauen- und Gesellschaftsbild des Ökofeminismus. Nach der Darstellung wesentlicher theoretischer Kategorien des Ökofeminismus, die sich zentrieren im Ideal der Hausarbeit als wichtigste Form von Subsistenzarbeit, stellt die Autorin zusammenfassend fest, daß der Ökofeminismus die gesellschaftlichen Unterschiede und historischen Veränderungen ignoriert, indem er den Weiblichkeitsbegriff aus der Gebärfähigkeit ableitet. Jede Ausbeutungsform wird reduziert auf biolog...

  6. Modelo no lineal basado en redes neuronales de unidades producto para clasificación. Una aplicación a la determinación del riesgo en tarjetas de crédito||Non-linear model for classification based on product-unit neural networks. An application to determine credit card risk

    Directory of Open Access Journals (Sweden)

    Martínez-Estudillo, F. J.

    2007-01-01

    Full Text Available El principal objetivo de este trabajo es mostrar un tipo de redes neuronales denominadas redes neuronales basadas en unidades producto (RNUP como un modelo no lineal que puede ser utilizado para la resolución de problemas de clasificación en aprendizaje. Proponemos un método evolutivo en el que simultáneamente se diseña la estructura de la red y se calculan los correspondientes pesos. La metodología que presentamos se basa, por tanto, en la combinación del modelo no lineal RNUP y del algoritmo evolutivo; se aplica a la resolución de un problema de clasificación de índole económica, surgido del mundo de las finanzas. Para evaluar el rendimiento de los modelos de clasificación obtenidos, comparamos nuestra propuesta con varias técnicas clásicas, como la regresión logística o el análisis discriminante, y con el clásico modelo de perceptrón multicapa de redes neuronales basado en unidades sigmoides y el algoritmo de aprendizaje de retropropagación (MLPBP.

  7. La fabrique des extraterrestres

    OpenAIRE

    Poulain, Sébastien

    2012-01-01

    Grâce à différentes stratégies discursives et médiatiques, Radio Ici et Maintenant joue un rôle dans la fabrication de la rumeur de l’existence des extraterrestres. Cette rumeur, teintée de complot, doit servir à légitimer l’utopie messianique et millénariste de cette radio New Age qui dispose de 5 000 auditeurs au quotidien. Cette radio prophétique et thérapeutique participe donc, jusqu’à un certain degré, au renouvellement de l’espace du politico-religieux. Thanks to various media and di...

  8. Therapie des Harnwegsinfekts

    Directory of Open Access Journals (Sweden)

    Stoiser B

    2010-01-01

    Full Text Available Harnwegsinfektionen gehören zu den häufigsten entzündlichen Erkrankungen im niedergelassenen Bereich. Eine intelligente Therapiestrategie stellt damit nicht nur eine klinische Herausforderung dar, sondern ermöglicht rasche Heilung, Vermeidung von Resistenzbildungen sowie oft unnötiger, teurer diagnostischer Schritte. Der erste entscheidende Schritt ist die genaue Klassifizierung des Harnweginfektes – asymptomatische Bakteriurie bis zur komplizierten Pyelonephritis. Hier entscheiden sich bereits Aggressivität der Behandlung sowie diagnostischer Aufwand. Für die Entwicklung einer empirischen antimikrobiellen Therapie ist die Kenntnis der häufigsten Erreger sowie lokaler Resistenzmuster wichtig. Bei gezieltem Vorgehen können die meisten Harnwegsinfekte ohne erhöhten diagnostischen Aufwand therapiert werden.

  9. LA TRADUCTION DES PREPOSITIONS

    Directory of Open Access Journals (Sweden)

    Samira MOUTAKIL

    2013-05-01

    Full Text Available L’idée de cet article nous est venue d’un cours qu’on assure pour les futurs guides touristiques en Jordanie. En traduisant des textes - français-arabe ou arabe-français, les étudiants se heurtent à certains problèmes pour rendre le texte vers la langue cible et qui soit convenable syntaxiquement et sémantiquement. On a remarqué donc que la traduction des prépositions vers l’une ou l’autre langue présente un dilemme pour les apprenants. On a décidé de composer un corpus de phrases contenant les propositions en français, « à » avec toutes ses formes, « dans et sur » et de l'arabe "على", "في" . Dans un premier temps, on a constitué le corpus avec 25 phrases dans les deux langues et on les a soumises aux étudiants, la liste en français aux étudiants du niveau A2 et la liste ne arabe aux étudiants du niveau A1. Par la suite, on a dégagé ce qui ressort de leurs traductions ce qui nous a permis de faire une analyse sémantico-syntaxique assez détaillée. En conclusion de cet article, on présente les résultats de cette expérience très intéressante.

  10. Marais Des Cygnes Wildlife Area

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This brochure is for the Marais des Cygnes Wildlife Area, managed by Kansas Department of Wildlife, Parks and Tourism, and located in the floodplain of the Marais...

  11. Digitalisierung des Kulturellen Erbes (Europas)

    NARCIS (Netherlands)

    Gruber, Marion

    2011-01-01

    Gruber, M. R. (2011, 13 December). Digitalisierung des Kulturellen Erbes (Europas). Guest lecture at the IPMZ - Institute of Mass Communication and Media Research, Devision Media Change & Innovation, University of Zurich, Switzerland.

  12. Systematically differentiating functions for alternatively spliced isoforms through integrating RNA-seq data.

    Science.gov (United States)

    Eksi, Ridvan; Li, Hong-Dong; Menon, Rajasree; Wen, Yuchen; Omenn, Gilbert S; Kretzler, Matthias; Guan, Yuanfang

    2013-01-01

    Integrating large-scale functional genomic data has significantly accelerated our understanding of gene functions. However, no algorithm has been developed to differentiate functions for isoforms of the same gene using high-throughput genomic data. This is because standard supervised learning requires 'ground-truth' functional annotations, which are lacking at the isoform level. To address this challenge, we developed a generic framework that interrogates public RNA-seq data at the transcript level to differentiate functions for alternatively spliced isoforms. For a specific function, our algorithm identifies the 'responsible' isoform(s) of a gene and generates classifying models at the isoform level instead of at the gene level. Through cross-validation, we demonstrated that our algorithm is effective in assigning functions to genes, especially the ones with multiple isoforms, and robust to gene expression levels and removal of homologous gene pairs. We identified genes in the mouse whose isoforms are predicted to have disparate functionalities and experimentally validated the 'responsible' isoforms using data from mammary tissue. With protein structure modeling and experimental evidence, we further validated the predicted isoform functional differences for the genes Cdkn2a and Anxa6. Our generic framework is the first to predict and differentiate functions for alternatively spliced isoforms, instead of genes, using genomic data. It is extendable to any base machine learner and other species with alternatively spliced isoforms, and shifts the current gene-centered function prediction to isoform-level predictions.

  13. Systematically differentiating functions for alternatively spliced isoforms through integrating RNA-seq data.

    Directory of Open Access Journals (Sweden)

    Ridvan Eksi

    Full Text Available Integrating large-scale functional genomic data has significantly accelerated our understanding of gene functions. However, no algorithm has been developed to differentiate functions for isoforms of the same gene using high-throughput genomic data. This is because standard supervised learning requires 'ground-truth' functional annotations, which are lacking at the isoform level. To address this challenge, we developed a generic framework that interrogates public RNA-seq data at the transcript level to differentiate functions for alternatively spliced isoforms. For a specific function, our algorithm identifies the 'responsible' isoform(s of a gene and generates classifying models at the isoform level instead of at the gene level. Through cross-validation, we demonstrated that our algorithm is effective in assigning functions to genes, especially the ones with multiple isoforms, and robust to gene expression levels and removal of homologous gene pairs. We identified genes in the mouse whose isoforms are predicted to have disparate functionalities and experimentally validated the 'responsible' isoforms using data from mammary tissue. With protein structure modeling and experimental evidence, we further validated the predicted isoform functional differences for the genes Cdkn2a and Anxa6. Our generic framework is the first to predict and differentiate functions for alternatively spliced isoforms, instead of genes, using genomic data. It is extendable to any base machine learner and other species with alternatively spliced isoforms, and shifts the current gene-centered function prediction to isoform-level predictions.

  14. ApoE isoform-dependent changes in hippocampal synaptic function

    Directory of Open Access Journals (Sweden)

    Sullivan Patrick M

    2009-05-01

    Full Text Available Abstract The lipoprotein receptor system in the hippocampus is intimately involved in the modulation of synaptic transmission and plasticity. The association of specific apoE isoform expression with human neurodegenerative disorders has focused attention on the role of these apoE isoforms in lipoprotein receptor-dependent synaptic modulation. In the present study, we used the apoE2, apoE3 and apoE4 targeted replacement (TR mice along with recombinant human apoE isoforms to determine the role of apoE isoforms in hippocampus area CA1 synaptic function. While synaptic transmission is unaffected by apoE isoform, long-term potentiation (LTP is significantly enhanced in apoE4 TR mice versus apoE2 TR mice. ApoE isoform-dependent differences in LTP induction require NMDA-receptor function, and apoE isoform expression alters activation of both ERK and JNK signal transduction. Acute application of specific apoE isoforms also alters LTP induction while decreasing NMDA-receptor mediated field potentials. Furthermore, acute apoE isoform application does not have the same effects on ERK and JNK activation. These findings demonstrate specific, isoform-dependent effects of human apoE isoforms on adult hippocampus synaptic plasticity and highlight mechanistic differences between chronic apoE isoform expression and acute apoE isoform exposure.

  15. Conformational Flexibility Differentiates Naturally Occurring Bet v 1 Isoforms.

    Science.gov (United States)

    Grutsch, Sarina; Fuchs, Julian E; Ahammer, Linda; Kamenik, Anna S; Liedl, Klaus R; Tollinger, Martin

    2017-06-03

    The protein Bet v 1 represents the main cause for allergic reactions to birch pollen in Europe and North America. Structurally homologous isoforms of Bet v 1 can have different properties regarding allergic sensitization and Th2 polarization, most likely due to differential susceptibility to proteolytic cleavage. Using NMR relaxation experiments and molecular dynamics simulations, we demonstrate that the initial proteolytic cleavage sites in two naturally occurring Bet v 1 isoforms, Bet v 1.0101 (Bet v 1a) and Bet v 1.0102 (Bet v 1d), are conformationally flexible. Inaccessible cleavage sites in helices and strands are highly flexible on the microsecond-millisecond time scale, whereas those located in loops display faster nanosecond-microsecond flexibility. The data consistently show that Bet v 1.0102 is more flexible and conformationally heterogeneous than Bet v 1.0101. Moreover, NMR hydrogen-deuterium exchange measurements reveal that the backbone amides in Bet v 1.0102 are significantly more solvent exposed, in agreement with this isoform's higher susceptibility to proteolytic cleavage. The differential conformational flexibility of Bet v 1 isoforms, along with the transient exposure of inaccessible sites to the protein surface, may be linked to proteolytic susceptibility, representing a potential structure-based rationale for the observed differences in Th2 polarization and allergic sensitization.

  16. Protein tyrosine phosphatase PTPRR isoforms in cellular signaling and trafficking

    NARCIS (Netherlands)

    Dilaver, Gönül

    2005-01-01

    Previous work has revealed the existence of two Protein Tyrosine Phosphatases in mouse, PTPBR7 and PTP-SL, that were in part identical, suggesting that they originated from the same gene, termed Ptprr (1,5,6). In this thesis, I report on the characterization of the various PTPRR isoforms in neuronal

  17. Murine Sirt3 protein isoforms have variable half-lives

    Science.gov (United States)

    Sirt3 is a NAD+-dependent protein deacetylase mainly localized in mitochondria. Recent studies indicate that the murine Sirt3 gene expresses different transcript variants resulting in three possible Sirt3 protein isoforms with variable lengths at the N-terminus: M1 (aa 1-334), M2 (aa 15-334), and M3...

  18. Isoforms of transferrin in psoriasis patients abusing alcohol

    NARCIS (Netherlands)

    P. Hoefkens (Peter); E.M. Higgins; R.J. Ward (Roberta); H.G. van Eijk (Henk)

    1997-01-01

    textabstractThe different isoforms of transferrin have been quantified by isoelectric focusing in the sera of psoriasis patients with and without a history of abusing alcohol. In both male and female psoriasis subjects abusing alcohol, there were significant increases in the 2-sial

  19. Differential water permeability and regulation of three aquaporin 4 isoforms

    DEFF Research Database (Denmark)

    Fenton, Robert A.; Moeller, Hanne B; Zelenina, Marina

    2010-01-01

    Aquaporin 4 (AQP4) is expressed in the perivascular glial endfeet and is an important pathway for water during formation and resolution of brain edema. In this study, we examined the functional properties and relative unit water permeability of three functional isoforms of AQP4 expressed in the b...

  20. Bacteria-Induced Dscam Isoforms of the Crustacean, Pacifastacus leniusculus.

    Directory of Open Access Journals (Sweden)

    Apiruck Watthanasurorot

    2011-06-01

    Full Text Available The Down syndrome cell adhesion molecule, also known as Dscam, is a member of the immunoglobulin super family. Dscam plays an essential function in neuronal wiring and appears to be involved in innate immune reactions in insects. The deduced amino acid sequence of Dscam in the crustacean Pacifastacus leniusculus (PlDscam, encodes 9(Ig-4(FNIII-(Ig-2(FNIII-TM and it has variable regions in the N-terminal half of Ig2 and Ig3 and the complete Ig7 and in the transmembrane domain. The cytoplasmic tail can generate multiple isoforms. PlDscam can generate more than 22,000 different unique isoforms. Bacteria and LPS injection enhanced the expression of PlDscam, but no response in expression occurred after a white spot syndrome virus (WSSV infection or injection with peptidoglycans. Furthermore, PlDscam silencing did not have any effect on the replication of the WSSV. Bacterial specific isoforms of PlDscam were shown to have a specific binding property to each tested bacteria, E. coli or S. aureus. The bacteria specific isoforms of PlDscam were shown to be associated with bacterial clearance and phagocytosis in crayfish.

  1. Distinct Functions of Endophilin Isoforms in Synaptic Vesicle Endocytosis

    Directory of Open Access Journals (Sweden)

    Jifeng Zhang

    2015-01-01

    Full Text Available Endophilin isoforms perform distinct characteristics in their interactions with N-type Ca2+ channels and dynamin. However, precise functional differences for the endophilin isoforms on synaptic vesicle (SV endocytosis remain unknown. By coupling RNA interference and electrophysiological recording techniques in cultured rat hippocampal neurons, we investigated the functional differences of three isoforms of endophilin in SV endocytosis. The results showed that the amplitude of normalized evoked excitatory postsynaptic currents in endophilin1 knockdown neurons decreased significantly for both single train and multiple train stimulations. Similar results were found using endophilin2 knockdown neurons, whereas endophilin3 siRNA exhibited no change compared with control neurons. Endophilin1 and endophilin2 affected SV endocytosis, but the effect of endophilin1 and endophilin2 double knockdown was not different from that of either knockdown alone. This result suggested that endophilin1 and endophilin2 functioned together but not independently during SV endocytosis. Taken together, our results indicate that SV endocytosis is sustained by endophilin1 and endophilin2 isoforms, but not by endophilin3, in primary cultured hippocampal neurons.

  2. Identification and characterization of a novel isoform of hepatopoietin

    Institute of Scientific and Technical Information of China (English)

    Jun Lu; Wang-Xiang Xu; Yi-Qun Zhan; Xiao-Lin Cui; Wei-Min Cai; Fu-Chu He; Xiao-Ming Yang

    2002-01-01

    AIM: To isolate a novel isoform of human HPO (HPO-205)human fetal liver Marathon-reedy cDNA andcharacterize its primary biological function.METHODS: 5'-RACE (rapid amplification of cDNA 5' ends)was used to isolate a novel isoform of hHPO in this paperThe constructed pcDNAHPO-205, pcDNAHPO and pcDNA eukaryotic expression vectors were respectively transfectedby lipofectamine method and the stimulation of DNAsynthesis was observed by 3H-TdR incorporation assay.Proteins extracted from different cells were analyzed byWestern blot.RESULTS: A novel isoform of hHPO (HPO-205) encoding a205 amino acid ORF corresponding to a translatedproduction of 23 kDa was isolated and distinguished fromthe previous HPO that lacked the N-terminal 80 amino acids.The dnse-dspendent stimulation of DNA synthesis of HepG2hepatoma cells by HPO-205 demonstrated its similarbiological activity with HPO in vitro. The level of MAPK(Mitogen-activated protein kinase) phnsphorylarion byWestern blot analysis revealed that HPO-205 might have thestronger activity of stimulating hepatic cell proliferation thanthat of HPO.CONCLUSION: A novel isoform of hHPO (HPO-205) wasisolated from hepatic-derived cells. The comparison of HPO-205 and HPO will lead to a new insight into the structure andfunction of hHPO, and provide the new way of thinking todeeply elucidate the biological roles of HPO/ALR.

  3. Cloning, expression and alternative splicing of the novel isoform of hTCP11 gene

    DEFF Research Database (Denmark)

    Ma, Yong-xin; Zhang, Si-zhong; Wu, Qia-qing;

    2003-01-01

    To identify a novel isoform of hTCP11 gene and investigate its expression and alternative splicing.......To identify a novel isoform of hTCP11 gene and investigate its expression and alternative splicing....

  4. Mast cells express novel functional IL-15 receptor alpha isoforms.

    Science.gov (United States)

    Bulanova, Elena; Budagian, Vadim; Orinska, Zane; Krause, Hans; Paus, Ralf; Bulfone-Paus, Silvia

    2003-05-15

    Mast cells previously have been reported to be regulated by IL-15 and to express a distinct IL-15R, termed IL-15RX. To further examine IL-15 binding and signaling in mast cells, we have studied the nature of the IL-15R and some of its biological activities in these cells. In this study, we report the existence of three novel isoforms of the IL-15R alpha chain in murine bone marrow-derived mast cells as a result of an alternative exon-splicing mechanism within the IL-15R alpha gene. These correspond to new mRNA transcripts lacking exon 4; exons 3 and 4; or exons 3, 4, and 5 (IL-15R alpha Delta 4, IL-15R alpha Delta 3,4, IL-15R alpha Delta 3,4,5). After transient transfection in COS-7 cells, all IL-15R alpha isoforms associate with the Golgi apparatus, the endoplasmic reticulum, the perinuclear space, and the cell membrane. Analysis of glycosylation pattern demonstrates the usage of a single N-glycosylation site, while no O-glycosylation is observed. Importantly, IL-15 binds with high affinity to, and promotes the survival of, murine BA/F3 cells stably transfected with the IL-15R alpha isoforms. Furthermore, we report that signaling mediated by IL-15 binding to the newly identified IL-15R alpha isoforms involves the phosphorylation of STAT3, STAT5, STAT6, Janus kinase 2, and Syk kinase. Taken together, our data indicate that murine mast cells express novel, fully functional IL-15R alpha isoforms, which can explain the selective regulatory effects of IL-15 on these cells.

  5. Growth hormone isoforms in a girl with gigantism.

    Science.gov (United States)

    Ng, L L; Chasalow, F I; Escobar, O; Blethen, S L

    1999-01-01

    Several previous investigations have suggested that there may be different growth hormone isoforms in patients with acromegaly. We used three different site-specific monoclonal antibodies (MAbs) to investigate growth hormone (GH) isoforms in serum from an 8 year-old girl with a GH and prolactin secreting adenoma. The pattern of GH-immunoreactivity was dependent on the circumstances of collection. Serum obtained after oral glucose had very little cross reactivity with MAb 352 although concentrations of up to 15 micrograms/l were found with two other MAbs, 033 and 665. MAb 352 does not recognize the 20,000 dalton isoform of GH (20K) while both MAb 033 and 665 do. The same pattern of GH immunoreactivity (low MAb 352, equal and higher MAb 033 and 665) was seen in other baseline samples. In contrast, samples obtained after TRH/GnRH showed immunoreactivity patterns expected for a mixture of 22,000 dalton isoform of GH (22K) with only a small amount of 20K. GH samples obtained during sleep showed both patterns with episodic peaks with equal immunoreactivity superimposed on the basal pattern (decreased activity with MAb 352). Affinity chromatography of basal samples showed that a portion of the GH immunoreactivity was neither 22K nor 20K, although in stimulated samples, over 70% of GH was 22K or 20K GH. In conclusion, the nature of GH isoforms present in serum varies with GH concentration. These differences may contribute to the known difficulty in correlating disease activity and random GH measurements in patients with GH secreting adenomas.

  6. Determination of the class and isoform selectivity of small-molecule histone deacetylase inhibitors

    DEFF Research Database (Denmark)

    Khan, N.; Jeffers, M.; Kumar, S.;

    2008-01-01

    ) against a panel of rhHDAC (recombinant human HDAC) isoforms. Eight rhHDACs were expressed using a baculoviral system, and a Fluor de Lystrade mark (Biomol International) HDAC assay was optimized for each purified isoform. The potency and selectivity of ten HDACs on class I isoforms (rhHDAC1, rhHDAC2, rh...

  7. Identification of cardiac myofilament protein isoforms using multiple mass spectrometry based approaches

    NARCIS (Netherlands)

    Kooij, V.; Venkatraman, V.; Kirk, J.A.; Ubaida-Mohien, C.; Graham, D.R.; Faber, M.J.; Eyk, J.E. Van

    2014-01-01

    PURPOSE: The identification of protein isoforms in complex biological samples is challenging. We, therefore, used an MS approach to unambiguously identify cardiac myofilament protein isoforms based on the observation of a tryptic peptide consisting of a sequence unique to a particular isoform. EXPER

  8. Expression, purification and enzymatic characterization of the catalytic domains of human tryptophan hydroxylase isoforms

    DEFF Research Database (Denmark)

    Windahl, Michael Skovbo; Boesen, Jane; Karlsen, Pernille Efferbach;

    2009-01-01

    Tryptophan hydroxylase exists in two isoforms: Isoform 1 catalyses the first and rate-limiting step in the synthesis of serotonin in the peripheral parts of the body while isoform 2 catalyses this step in the brain. The catalytic domains of human tryptophan hydroxylase 1 and 2 have been expressed...

  9. Terre des hommes

    CERN Multimedia

    Staff Association

    2012-01-01

    Transformez votre téléphone portable en geste de solidarité ! Collecte du 12 au 23 novembre 2012   Faites un geste simple et utile en déposant vos téléphones portables inutilisés dans les urnes installées dans les trois restaurants du CERN. En Suisse, une personne change tous les 12 à 18 mois de téléphone portable. La plupart de nos vieux appareils sont simplement laissés à l’abandon avec comme seule fonction de parer une éventuelle panne à venir. On  estime ainsi que 8 millions de portables sont inutilisés, alors qu'entre 30 et 50% peuvent être réutilisés. L'action Solidarcomm leur offre une deuxième vie ! Terre des Hommes Suisse, dans le cadre de la campagne Solidarcomm, collecte et valorise vos téléphones inutilis&...

  10. Onlinespieler abseits des Mainstreams

    Directory of Open Access Journals (Sweden)

    Harald Baumgartlinger

    2012-12-01

    Full Text Available MMO(RPGs „Massively Multiplayer Online (Role-Playing Games“ nehmen einen Sonderstatus unter den Onlinespielen ein, welcher durch den wachsenden wirtschaftlichen Erfolg, insbesondere durch den „breakthrough hit“ (Duchenaut et al. 2006: 407 World of Warcraft (WoW, auch an Bedeutung für die Medien- und Kommunikationswissenschaft gewinnt (vgl. Inderst 2009: 15; vgl. Seifert/Jöckel 2008: 297. Der Primus des Genres, WoW, repräsentiert gemeinsam mit einigen nahezu identen Titeln insgesamt 85 Prozent der gespielten MMOGs (vgl. Williams et al. 2008: 999. Daher befasst sich auch die Mehrheit der vorliegenden Studien mit eben diesen Spielen. Neue Formen von MMO(Gs können jedoch zu ebenso neuartigen Spielerfahrungen führen und damit unterschiedliche Nutzungsmotive befriedigen (vgl. Seifert/Jöckel 2008: 309 und folglich zu einem gänzlich anderen Spielerleben führen. Während im Mainstream die kooperativen Spielerbeziehungen überwiegen, dominiert in Darkfall Online der soziale Wettbewerb. Der von Williams et al. 2008 verwendete Fragebogen diente als Rohling für die Kreation eines für die speziellen Anforderungen adaptierten Erhebungstools zur Erforschung der Spielertypologie und der Motive der Darkfall-Online-SpielerInnen. Die in der Onlinebefragung (N = 506 gesammelten Daten belegen, dass sich sowohl die Spielerdemographie, als auch die Motive der Spieler von den Mainstream MMOs unterscheiden. Zudem konnten realweltliche Eigenschaften der Spieler als signifikante Einflussfaktoren für die Spielzuwendung identifiziert werden.

  11. Peste des petits ruminants.

    Science.gov (United States)

    Parida, S; Muniraju, M; Mahapatra, M; Muthuchelvan, D; Buczkowski, H; Banyard, A C

    2015-12-14

    Peste des petits ruminants virus causes a highly infectious disease of small ruminants that is endemic across Africa, the Middle East and large regions of Asia. The virus is considered to be a major obstacle to the development of sustainable agriculture across the developing world and has recently been targeted by the World Organisation for Animal Health (OIE) and the Food and Agriculture Organisation (FAO) for eradication with the aim of global elimination of the disease by 2030. Fundamentally, the vaccines required to successfully achieve this goal are currently available, but the availability of novel vaccine preparations to also fulfill the requisite for differentiation between infected and vaccinated animals (DIVA) may reduce the time taken and the financial costs of serological surveillance in the later stages of any eradication campaign. Here, we overview what is currently known about the virus, with reference to its origin, updated global circulation, molecular evolution, diagnostic tools and vaccines currently available to combat the disease. Further, we comment on recent developments in our knowledge of various recombinant vaccines and on the potential for the development of novel multivalent vaccines for small ruminants. Copyright © 2015 The Authors. Published by Elsevier B.V. All rights reserved.

  12. CCC/WPA study : Des Lacs NWR

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — Summary of the Civilian Conservation Corps (CCC) camp at Des Lacs National Wildlife Refuge from July 1935-May 1942 to carry on restoration and development of Des...

  13. EGFR soluble isoforms and their transcripts are expressed in meningiomas.

    Science.gov (United States)

    Guillaudeau, Angélique; Durand, Karine; Bessette, Barbara; Chaunavel, Alain; Pommepuy, Isabelle; Projetti, Fabrice; Robert, Sandrine; Caire, François; Rabinovitch-Chable, Hélène; Labrousse, François

    2012-01-01

    The EGFR (epidermal growth factor receptor) is involved in the oncogenesis of many tumors. In addition to the full-length EGFR (isoform a), normal and tumor cells produce soluble EGFR isoforms (sEGFR) that lack the intracellular domain. sEGFR isoforms b, c and d are encoded by EGFR variants 2 (v2), 3 (v3) and 4 (v4) mRNA resulting from gene alternative splicing. Accordingly, the results of EGFR protein expression analysis depend on the domain targeted by the antibodies. In meningiomas, EGFR expression investigations mainly focused on EGFR isoform a. sEGFR and EGFRvIII mutant, that encodes a constitutively active truncated receptor, have not been studied. In a 69 meningiomas series, protein expression was analyzed by immunohistochemistry using extracellular domain targeted antibody (ECD-Ab) and intracellular domain targeted antibody (ICD-Ab). EGFRv1 to v4 and EGFRvIII mRNAs were quantified by RT-PCR and EGFR amplification revealed by MLPA. Results were analyzed with respect to clinical data, tumor resection (Simpson grade), histological type, tumor grade, and patient outcome.Immunochemical staining was stronger with ECD-Ab than with ICD-Ab. Meningiomas expressed EGFRv1 to -v4 mRNAs but not EGFRvIII mutant. Intermediate or high ECD-Ab staining and high EGFRv1 to v4 mRNA levels were associated to a better progression free survival (PFS). PFS was also improved in women, when tumor resection was evaluated as Simpson 1 or 2, in grade I vs. grade II and III meningiomas and when Ki67 labeling index was lower than 10%. Our results suggest that, EGFR protein isoforms without ICD and their corresponding mRNA variants are expressed in meningiomas in addition to the whole isoform a. EGFRvIII was not expressed. High expression levels seem to be related to a better prognosis. These results indicate that the oncogenetic mechanisms involving the EGFR pathway in meningiomas could be different from other tumor types.

  14. EGFR soluble isoforms and their transcripts are expressed in meningiomas.

    Directory of Open Access Journals (Sweden)

    Angélique Guillaudeau

    Full Text Available The EGFR (epidermal growth factor receptor is involved in the oncogenesis of many tumors. In addition to the full-length EGFR (isoform a, normal and tumor cells produce soluble EGFR isoforms (sEGFR that lack the intracellular domain. sEGFR isoforms b, c and d are encoded by EGFR variants 2 (v2, 3 (v3 and 4 (v4 mRNA resulting from gene alternative splicing. Accordingly, the results of EGFR protein expression analysis depend on the domain targeted by the antibodies. In meningiomas, EGFR expression investigations mainly focused on EGFR isoform a. sEGFR and EGFRvIII mutant, that encodes a constitutively active truncated receptor, have not been studied. In a 69 meningiomas series, protein expression was analyzed by immunohistochemistry using extracellular domain targeted antibody (ECD-Ab and intracellular domain targeted antibody (ICD-Ab. EGFRv1 to v4 and EGFRvIII mRNAs were quantified by RT-PCR and EGFR amplification revealed by MLPA. Results were analyzed with respect to clinical data, tumor resection (Simpson grade, histological type, tumor grade, and patient outcome.Immunochemical staining was stronger with ECD-Ab than with ICD-Ab. Meningiomas expressed EGFRv1 to -v4 mRNAs but not EGFRvIII mutant. Intermediate or high ECD-Ab staining and high EGFRv1 to v4 mRNA levels were associated to a better progression free survival (PFS. PFS was also improved in women, when tumor resection was evaluated as Simpson 1 or 2, in grade I vs. grade II and III meningiomas and when Ki67 labeling index was lower than 10%. Our results suggest that, EGFR protein isoforms without ICD and their corresponding mRNA variants are expressed in meningiomas in addition to the whole isoform a. EGFRvIII was not expressed. High expression levels seem to be related to a better prognosis. These results indicate that the oncogenetic mechanisms involving the EGFR pathway in meningiomas could be different from other tumor types.

  15. Current algebra; Algebre des courants

    Energy Technology Data Exchange (ETDEWEB)

    Jacob, M. [Commissariat a l' Energie Atomique, Saclay (France). Centre d' Etudes Nucleaires

    1967-07-01

    The first three chapters of these lecture notes are devoted to generalities concerning current algebra. The weak currents are defined, and their main properties given (V-A hypothesis, conserved vector current, selection rules, partially conserved axial current,...). The SU (3) x SU (3) algebra of Gell-Mann is introduced, and the general properties of the non-leptonic weak Hamiltonian are discussed. Chapters 4 to 9 are devoted to some important applications of the algebra. First one proves the Adler- Weisberger formula, in two different ways, by either the infinite momentum frame, or the near-by singularities method. In the others chapters, the latter method is the only one used. The following topics are successively dealt with: semi leptonic decays of K mesons and hyperons, Kroll- Ruderman theorem, non leptonic decays of K mesons and hyperons ( {delta}I = 1/2 rule), low energy theorems concerning processes with emission (or absorption) of a pion or a photon, super-convergence sum rules, and finally, neutrino reactions. (author) [French] La premiere partie de ce cours (trois premiers chapitres), traite des generalites concernant l'algebre de courants. Apres une definition rapide des courants faibles et un rappel de leurs proprietes (hypothese V-A, conservation du courant vecteur, regles de selection, courant axial partiellement conserve,...), l'on introduit l'algebre de Gell-Mann SU (3) x SU (3), et discute les proprietes generales de l'Hamiltonien faible non leptonique. Les chapitres IV a IX sont consacres a des applications importantes de l'algebre des courants. En premier lieu l'on demontre la formule de Adler et Weisberger, par deux methodes differentes, celle dite du repere de moment infini et celle des singularites proches. Cette derniere est seule utilisee dans la suite. Puis, l'on traite successivement les problemes suivants: desintegrations semi-leptoniques des mesons K et des hyperons, theoreme de Kroll

  16. La théorie des industries culturelles (et informationnelles, composante des SIC

    Directory of Open Access Journals (Sweden)

    Bernard Miège

    2012-09-01

    Full Text Available Inaugurer le Cahier central de la Revue des SIC est certes une forme de reconnaissance, mais cela comporte des obligations, au premier rang desquelles la nécessité d’intéresser des lecteurs a priori pas immédiatement concernés par la thématique.Si la théorie des industries culturelles est devenue progressivement une approche marquante des SIC, en France, en Europe et plus largement encore (avec des dénominations variables et des modalités spécifiques, ce n’est en effet ni en le proclamant ha...

  17. Neologismen des Lettischen anhand des schriftstellerischen Werkes von Zigmunds Skujins

    OpenAIRE

    Javojss, Skaidrite

    2010-01-01

    Untersuchung: Aus acht Werken des zeitgenössischen Schriftstellers Zigmunds Skujins wurden 500 lettische Wörter untersucht, die in den drei umfangreichsten lettischen Wörterbüchern nicht verzeichnet und somit möglicherweise Neologismen des Autors sind. Analysiert werden die Wortarten, die Komposita, die Herkunft der assimilierten Lehnwörter, orthographische und andere Varianten bereits lexikalisierter Wörter, die Arten der Diminutivbildung und der Präfigierung. Außerdem werden Aussagen über d...

  18. Estimación de la resistencia a la penetración de suelos usando redes neuronales artificiales Prediction of the soils penetration strength using artificial neural networks

    Directory of Open Access Journals (Sweden)

    Nidia Johana Valdés Holguín

    2011-07-01

    Full Text Available Las redes neuronales artificiales, simuladoras del proceso de aprendizaje de las neuronas biológicas, han sido utilizadas con éxito en el cálculo de parámetros en diversos problemas de ingeniería en que las variables involucradas tienen una alta relación no lineal entre sí y la modelación no permite representar el problema mediante una función matemática de fácil deducción. En la ciencia del suelo la predicción de algunas propiedades involucra diversas variables que hacen de su estimación por medio de modelos matemáticos un proceso complejo, y trasladan la solución del problema al campo de la inteligencia artificial. En el presente artículo se reporta la elaboración de redes neuronales artificiales para la estimación de la resistencia a la penetración a diferentes profundidades de un suelo; se consideran como variables influyentes el contenido de humedad, la densidad, la carga estática y la presión de inflado. Los resultados muestran una mejor estimación para profundidades entre 20 cm y 30 cm.Artificial Neural Networks simulate the learning process of biological neurons, and these have been successfully used in the computation of parameters on several engineering problems where exist a strong nonlinear relation among the variables. In soil science, estimation of some properties involves variables that are complicated to estimate using mathematical models, so the solution for the problems fall into the field of Artificial Intelligence. The present paper reports the elaboration of an Artificial Neural Network for the estimation of penetration resistance of soil at different depths, considering as influential variables humidity, density, static load, and inflate pressure. The best estimation results were obtained at a depth of 20-30 cm.

  19. Proteomic Validation of Transcript Isoforms, Including Those Assembled from RNA-Seq Data

    DEFF Research Database (Denmark)

    Tay, Aidan P; Pang, Chi Nam Ignatius; Twine, Natalie a;

    2015-01-01

    data, and proteomic analysis of the same sample, can identify protein isoforms. RNA-seq data from human mesenchymal (hMSC) stem cells were analyzed with our new TranscriptCoder tool to generate a database of protein isoform sequences. MS/MS data from matching hMSC samples were then matched against...... the TranscriptCoder-derived database, along with Ensembl and the neXtProt database. Querying the TranscriptCoder-derived or Ensembl database could unambiguously identify ∼450 protein isoforms, with isoform-specific proteotypic peptides, including candidate hMSC-specific isoforms for the genes DPYSL2 and FXR1...

  20. Evaluer des études de gestion des entreprises : combiner des éléments principales avec des éléments pratiques

    NARCIS (Netherlands)

    Pennink, B.J.W.

    2002-01-01

    Evaluer la recherche de gestion demande une combinaison des critères classiques et des critères pratiques. Par cette combinaison il est possible de trouver une réponse cerrecte concernant la validité et l'effet de la recherche. Dans cet article nous allons décrire comment une combinaison des

  1. Characterization of four hemocyanin isoforms in Litopenaeus vannamei

    Institute of Scientific and Technical Information of China (English)

    XU Jingxiang; RUAN Lingwei; LI Zhen; YU Xiaoman; LI Sedong; SHI Hong; XU Xun

    2015-01-01

    In this study, the gene encoding hemocyanin subunit L, LvHcL, was cloned from Litopenaeus vannamei and the genomic organization was characterized. This gene was diverse with many SNPs and also had at least four isoforms, while one of them (LvHcL4) only had two exons and the exon2 was missed. Transcription analysis showed that these isoforms of LvHcL were up-regulated after WSSV challenge in WSSV-resistant shrimp, while the transcriptions were decreased constantly in WSSV-susceptible shrimp. It is suggested that the hemocyanin had rich polymorphism and was involved in the antiviral response. These results could extend our previous findings and provide insights into the immune feature of hemocyanin, which would be helpful for further studies aimed at antiviral mechanism in inver-tebrate.

  2. Disulfide isoforms of recombinant glia maturation factor beta.

    Science.gov (United States)

    Zaheer, A; Lim, R

    1990-09-14

    Recombinant human glia maturation factor beta (r-hGMF-beta) is a single-chain polypeptide (141 amino acid residues) containing three cysteines, at positions 7, 86 and 95. Nascent r-hGMF-beta exists in the reduced state and has no biological activity. The protein can be activated through oxidative refolding by incubation with a mixture of reduced and oxidized glutathione. Reverse-phase HPLC analysis of the refolded r-hGMF-beta shows the presence of four peaks, corresponding to the reduced form plus three newly generated intrachain disulfide-containing isoforms predicted from the number of cysteine residues. Only one isoform shows biological activity when tested for growth suppression on C6 glioma cells. We infer from the HPLC elution pattern that the active form contains the disulfide bridge Cys86-Cys95.

  3. Alimentation des tortues terrestres herbivores

    OpenAIRE

    Morin, Nicolas

    2015-01-01

    Chez les tortues, la plupart des affections rencontrées sont directement ou indirectement liées à un défaut dans les conditions d’entretien et notamment à une alimentation inadéquate. Les ouvrages de référence ne proposent généralement que des rations qualitatives déterminées empiriquement. Ce travail se veut une synthèse bibliographique des différents travaux qui ont été menés sur les tortues terrestres herbivores, dont le but est de dégager les paramètres physiologiques sous-jacents au ...

  4. LES APPROCHES PSYCHOSOCIOLOGIQUES DES ORGANISATIONS

    Directory of Open Access Journals (Sweden)

    Deaconu Alecxandrina

    2008-05-01

    Full Text Available Les préoccupations pour bien comprendre la complexité des organisations sont bien connues dans la théorie et la pratique du management. La motivation la plus fréquente pour toutes les recherches et les investigationes faites a été fondée sur le besoin de savoir gérer les situations diverses en vue de maximiser la performance organisationnelle. En ce qui nous concerne, pour enrichir les informations disponibles, nous voulons élargir, dans notre communication, les approches traditionelles, focaliser l’attention sur la dimension psychologiques des organisations et présenter les mécanismes qui favorisent l’implication des salariés.

  5. L’Internet des objets

    OpenAIRE

    Benghozi, Pierre-Jean; Bureau, Sylvain; Massit-Folléa, Françoise

    2012-01-01

    L’ « internet des objets » est une dimension majeure de l’internet du futur. Mais tout le monde ne s’accorde pas encore sur sa définition, ni sur la mesure de son importance économique ou des risques qu’il induit. L’étude de nombreux rapports prospectifs et l’observation des innovations d’ores et déjà engagées a permis de mettre en relief les incertitudes techniques, économiques et socio-politiques qui pèsent sur cette véritable mutation programmée de l’internet et de proposer une approche eu...

  6. L’Internet des objets

    OpenAIRE

    Benghozi, Pierre-Jean; Bureau, Sylvain; Massit-Folléa, Françoise

    2012-01-01

    L’ « internet des objets » est une dimension majeure de l’internet du futur. Mais tout le monde ne s’accorde pas encore sur sa définition, ni sur la mesure de son importance économique ou des risques qu’il induit. L’étude de nombreux rapports prospectifs et l’observation des innovations d’ores et déjà engagées a permis de mettre en relief les incertitudes techniques, économiques et socio-politiques qui pèsent sur cette véritable mutation programmée de l’internet et de proposer une approche eu...

  7. Method for analysing glycoprotein isoforms by capillary electrophoresis

    OpenAIRE

    Frutos, Mercedes de; Díez-Masa, José Carlos; Morales-Cid, Gabriel

    2011-01-01

    [EN] The present invention relates to a new method for the purification, concentration, separation and determination of the isoforms of alpha-1-acid glycoprotein (AGP) in human blood serum samples using capillary electrophoresis. The new method is based on the immunocapture and preconcentration of the sample within the separation capillary by using an immunoadsorbent phase magnetically immobilized within the electrophoresis capillary and the subsequent desorption and separation of the glycopr...

  8. Functional differences between L- and T-plastin isoforms.

    Science.gov (United States)

    Arpin, M; Friederich, E; Algrain, M; Vernel, F; Louvard, D

    1994-12-01

    Fimbrins/plastins are a family of highly conserved actin-bundling proteins. They are present in all eukaryotic cells including yeast, but each isoform displays a remarkable tissue specificity. T-plastin is normally found in epithelial and mesenchymal cells while L-plastin is present in hematopoietic cells. However, L-plastin has been also found in tumor cells of non-hematopoietic origin (Lin, C.-S., R. H. Aebersold, S. B. Kent, M. Varma, and J. Leavitt. 1988. Mol. Cell. Biol. 8:4659-4668; Lin, C.-S., R. H. Aebersold, and J. Leavitt. 1990. Mol. Cell. Biol. 10: 1818-1821). To learn more about the biological significance of their tissue specificity, we have overproduced the T- and L-plastin isoforms in a fibroblast-like cell line, CV-1, and in a polarized epithelial cell line, LLC-PK1. In CV-1 cells, overproduction of T- and L-plastins induces cell rounding and a concomitant reorganization of actin stress fibers into geodesic structures. L-plastin remains associated with microfilaments while T-plastin is almost completely extracted after treatment of the cells with non-ionic detergent. In LLC-PK1 cells, T-plastin induces shape changes in microvilli and remains associated with microvillar actin filaments after detergent extraction while L-plastin has no effect on these structures and is completely extracted. The effect of T-plastin on the organization of microvilli differs from that of villin, another actin-bundling protein. Our experiments indicate that these two isoforms play differing roles in actin filament organization, and do so in a cell type-specific fashion. Thus it is likely that these plastin isoforms play fundamentally different roles in cell function.

  9. [Clinical relevance of myosin isoforms in the diaphragm].

    Science.gov (United States)

    Gayan-Ramirez, G; Decramer, M

    2000-06-01

    The diaphragm as a striated muscle is characterized by the repetition of a single element arranged in series: the sarcomere containing two kinds of myofilaments: a thick one constituted by the myosin, and a thin one primarily composed of actin. The myosin molecule consists of two heads where two myosin heavy chains (MHC) are fixed, a flexible hinge with two light (MLC) chains, and long rod-shaped tails. The diaphragm contains 4 MHC isoforms (MHC-slow, MHC-2A, MHC-2B, MHC-2X) and 6 MLC isoforms (MLC-1f, MLC-3f, MLC-1sa, MLC-1sb, MLC-2f, MLC-2s/v). In humans, the diaphragm contains mainly fibers expressing the isoforms MHC-slow, MHC-2A, and MLC-2f, MLC-2s et MLC-1f. For the mechanical properties of the different isoforms, there is a gradient from the MHC-slow to the MHC-2A, MHC-2B and MHC-2X/2B. According to the circumstances, the diaphragm will adapt towards a slow profile (COPD, cardiac failure and in animals: Duchenne muscular dystrophy, denervation-1 week, age-female, corticosteroids, chronic stimulation), or a fast profile (in animals: chronic hypoxia, denervation-2 weeks, age-males) or a more oxidative profile (in animals: cachexia, obesity). The reasons why the diaphragm adapts towards a slower or a faster muscle are not known. In fact, for a given pathological situation, several factors are able to influence the fiber composition of the diaphragm. Therefore, the net result of the influence of these different factors in terms of MHC and MLC diaphragm adaptation is difficult to predict.

  10. Characterization of the Human LPIN1-encoded Phosphatidate Phosphatase Isoforms*

    Science.gov (United States)

    Han, Gil-Soo; Carman, George M.

    2010-01-01

    The human LPIN1 gene encodes the protein lipin 1, which possesses phosphatidate (PA) phosphatase (3-sn-phosphatidate phosphohydrolase; EC 3.1.3.4) activity (Han, G.-S., Wu, W.-I., and Carman, G. M. (2006) J. Biol. Chem. 281, 9210–9218). In this work, we characterized human lipin 1 α, β, and γ isoforms that were expressed in Escherichia coli and purified to near homogeneity. PA phosphatase activities of the α, β, and γ isoforms were dependent on Mg2+ or Mn2+ ions at pH 7.5 at 37 °C. The activities were inhibited by concentrations of Mg2+ and Mn2+ above their optimums and by Ca2+, Zn2+, N-ethylmaleimide, propranolol, and the sphingoid bases sphingosine and sphinganine. The activities were thermally labile at temperatures above 40 °C. The α, β, and γ activities followed saturation kinetics with respect to the molar concentration of PA (Km values of 0.35, 0.24, and 0.11 mm, respectively) but followed positive cooperative (Hill number ∼2) kinetics with respect to the surface concentration of PA (Km values of 4.2, 4.5, and 4.3 mol %, respectively) in Triton X-100/PA-mixed micelles. The turnover numbers (kcat) for the α, β, and γ isoforms were 68.8 ± 3.5, 42.8 ± 2.5, and 5.7 ± 0.2 s−1, respectively, whereas their energy of activation values were 14.2, 15.5, and 18.5 kcal/mol, respectively. The isoform activities were dependent on PA as a substrate and required at least one unsaturated fatty acyl moiety for maximum activity. PMID:20231281

  11. Regulation of NADPH oxidase 5 by protein kinase C isoforms.

    Directory of Open Access Journals (Sweden)

    Feng Chen

    Full Text Available NADPH oxidase5 (Nox5 is a novel Nox isoform which has recently been recognized as having important roles in the pathogenesis of coronary artery disease, acute myocardial infarction, fetal ventricular septal defect and cancer. The activity of Nox5 and production of reactive oxygen species is regulated by intracellular calcium levels and phosphorylation. However, the kinases that phosphorylate Nox5 remain poorly understood. Previous studies have shown that the phosphorylation of Nox5 is PKC dependent, but this contention was based on the use of pharmacological inhibitors and the isoforms of PKC involved remain unknown. Thus, the major goals of this study were to determine whether PKC can directly regulate Nox5 phosphorylation and activity, to identify which isoforms are involved in the process, and to understand the functional significance of this pathway in disease. We found that a relatively specific PKCα inhibitor, Ro-32-0432, dose-dependently inhibited PMA-induced superoxide production from Nox5. PMA-stimulated Nox5 activity was significantly reduced in cells with genetic silencing of PKCα and PKCε, enhanced by loss of PKCδ and the silencing of PKCθ expression was without effect. A constitutively active form of PKCα robustly increased basal and PMA-stimulated Nox5 activity and promoted the phosphorylation of Nox5 on Ser490, Thr494, and Ser498. In contrast, constitutively active PKCε potently inhibited both basal and PMA-dependent Nox5 activity. Co-IP and in vitro kinase assay experiments demonstrated that PKCα directly binds to Nox5 and modifies Nox5 phosphorylation and activity. Exposure of endothelial cells to high glucose significantly increased PKCα activation, and enhanced Nox5 derived superoxide in a manner that was in prevented by a PKCα inhibitor, Go 6976. In summary, our study reveals that PKCα is the primary isoform mediating the activation of Nox5 and this maybe of significance in our understanding of the vascular

  12. Isoform expression in the multiple soluble malate dehydrogenase of Hoplias malabaricus (Erythrinidae, Characiformes

    Directory of Open Access Journals (Sweden)

    M. R. Aquino-Silva

    Full Text Available Kinetic properties and thermal stabilities of Hoplias malabaricus liver and skeletal muscle unfractionated malate dehydrogenase (MDH, EC 1.1.1.37 and its isolated isoforms were analyzed to further study the possible sMDH-A* locus duplication evolved from a recent tandem duplication. Both A (A1 and A2 and B isoforms had similar optima pH (7.5-8.0. While Hoplias A isoform could not be characterized as thermostable, B could as thermolabile. A isoforms differed from B isoform in having higher Km values for oxaloacetate. The possibly duplicated A2 isoform showed higher substrate affinity than the A1. Hoplias duplicated A isoforms may influence the direction of carbon flow between glycolisis and gluconeogenesis.

  13. Isoform expression in the multiple soluble malate dehydrogenase of Hoplias malabaricus (Erythrinidae, Characiformes

    Directory of Open Access Journals (Sweden)

    Aquino-Silva M. R.

    2003-01-01

    Full Text Available Kinetic properties and thermal stabilities of Hoplias malabaricus liver and skeletal muscle unfractionated malate dehydrogenase (MDH, EC 1.1.1.37 and its isolated isoforms were analyzed to further study the possible sMDH-A* locus duplication evolved from a recent tandem duplication. Both A (A1 and A2 and B isoforms had similar optima pH (7.5-8.0. While Hoplias A isoform could not be characterized as thermostable, B could as thermolabile. A isoforms differed from B isoform in having higher Km values for oxaloacetate. The possibly duplicated A2 isoform showed higher substrate affinity than the A1. Hoplias duplicated A isoforms may influence the direction of carbon flow between glycolisis and gluconeogenesis.

  14. Characterisation of CDKL5 Transcript Isoforms in Human and Mouse.

    Directory of Open Access Journals (Sweden)

    Ralph D Hector

    Full Text Available Mutations in the X-linked Cyclin-Dependent Kinase-Like 5 gene (CDKL5 cause early onset infantile spasms and subsequent severe developmental delay in affected children. Deleterious mutations have been reported to occur throughout the CDKL5 coding region. Several studies point to a complex CDKL5 gene structure in terms of exon usage and transcript expression. Improvements in molecular diagnosis and more extensive research into the neurobiology of CDKL5 and pathophysiology of CDKL5 disorders necessitate an updated analysis of the gene. In this study, we have analysed human and mouse CDKL5 transcript patterns both bioinformatically and experimentally. We have characterised the predominant brain isoform of CDKL5, a 9.7 kb transcript comprised of 18 exons with a large 6.6 kb 3'-untranslated region (UTR, which we name hCDKL5_1. In addition we describe new exonic regions and a range of novel splice and UTR isoforms. This has enabled the description of an updated gene model in both species and a standardised nomenclature system for CDKL5 transcripts. Profiling revealed tissue- and brain development stage-specific differences in expression between transcript isoforms. These findings provide an essential backdrop for the diagnosis of CDKL5-related disorders, for investigations into the basic biology of this gene and its protein products, and for the rational design of gene-based and molecular therapies for these disorders.

  15. Characterisation of CDKL5 Transcript Isoforms in Human and Mouse.

    Science.gov (United States)

    Hector, Ralph D; Dando, Owen; Landsberger, Nicoletta; Kilstrup-Nielsen, Charlotte; Kind, Peter C; Bailey, Mark E S; Cobb, Stuart R

    2016-01-01

    Mutations in the X-linked Cyclin-Dependent Kinase-Like 5 gene (CDKL5) cause early onset infantile spasms and subsequent severe developmental delay in affected children. Deleterious mutations have been reported to occur throughout the CDKL5 coding region. Several studies point to a complex CDKL5 gene structure in terms of exon usage and transcript expression. Improvements in molecular diagnosis and more extensive research into the neurobiology of CDKL5 and pathophysiology of CDKL5 disorders necessitate an updated analysis of the gene. In this study, we have analysed human and mouse CDKL5 transcript patterns both bioinformatically and experimentally. We have characterised the predominant brain isoform of CDKL5, a 9.7 kb transcript comprised of 18 exons with a large 6.6 kb 3'-untranslated region (UTR), which we name hCDKL5_1. In addition we describe new exonic regions and a range of novel splice and UTR isoforms. This has enabled the description of an updated gene model in both species and a standardised nomenclature system for CDKL5 transcripts. Profiling revealed tissue- and brain development stage-specific differences in expression between transcript isoforms. These findings provide an essential backdrop for the diagnosis of CDKL5-related disorders, for investigations into the basic biology of this gene and its protein products, and for the rational design of gene-based and molecular therapies for these disorders.

  16. Differential expression of syndecan isoforms during mouse incisor amelogenesis.

    Science.gov (United States)

    Muto, Taro; Miyoshi, Keiko; Munesue, Seiichi; Nakada, Hiroshi; Okayama, Minoru; Matsuo, Takashi; Noma, Takafumi

    2007-08-01

    Syndecans are transmembranous heparan sulfate proteoglycans (HSPGs) with covalently attached glycosaminoglycan side-chains located on the cell surface. The mammalian syndecan family is composed of four types of syndecans (syndecan-1 to -4). Syndecans interact with the intracellular cytoskeleton through the cytoplasmic domains of their core proteins and membrane proteins, extracellular enzymes, growth factors, and matrix components, through their heparan-sulfate chains, to regulate developmental processes.Here, as a first step to assess the possible roles of syndecan proteins in amelogenesis, we examined the expression patterns of all syndecan isoforms in continuously growing mouse incisors, in which we can overview major differentiation stages of amelogenesis at a glance. Understanding the expression domain of each syndecan isoform during specific developmental stages seems useful for investigating their physiological roles in amelogenesis. Immunohistochemical analysis of syndecan core proteins in the lower incisors from postnatal day 1 mice revealed spatially and temporally specific expression patterns, with syndecan-1 expressed in undifferentiated epithelial and mesenchymal cells, and syndecan-2, -3, and -4 in more differentiated cells. These findings suggest that each syndecan isoform functions distinctly during the amelogenesis of the incisors of mice.

  17. Antagonistic functions of two stardust isoforms in Drosophila photoreceptor cells.

    Science.gov (United States)

    Bulgakova, Natalia A; Rentsch, Michaela; Knust, Elisabeth

    2010-11-15

    Membrane-associated guanylate kinases (MAGUKs) are scaffolding proteins that organize supramolecular protein complexes, thereby partitioning the plasma membrane into spatially and functionally distinct subdomains. Their modular organization is ideally suited to organize protein complexes with cell type- or stage-specific composition, or both. Often more than one MAGUK isoform is expressed by one gene in the same cell, yet very little is known about their individual in vivo functions. Here, we show that two isoforms of Drosophila stardust, Sdt-H (formerly called Sdt-B2) and Sdt-D, which differ in their N terminus, are expressed in adult photoreceptors. Both isoforms associate with Crumbs and PATJ, constituents of the conserved Crumbs-Stardust complex. However, they form distinct complexes, localized at the stalk, a restricted region of the apical plasma membrane. Strikingly, Sdt-H and Sdt-D have antagonistic functions. While Sdt-H overexpression increases stalk membrane length and prevents light-dependent retinal degeneration, Sdt-D overexpression reduces stalk length and enhances light-dependent retinal degeneration. These results suggest that a fine-tuned balance of different Crumbs complexes regulates photoreceptor homeostasis.

  18. Géographie des cryptarchies

    Directory of Open Access Journals (Sweden)

    Gilles Fumey

    2002-06-01

    Full Text Available Si toute la surface du globe est appropriée, certains territoires sont encore revendiqués par des personnes ou petits groupes usurpateurs, conquérants ou aventuriers. Leur «conquête» porte sur des espaces physiques ou virtuels (internet. Elle traduit avec constance dans l'histoire le rôle considérable de l'ancrage territorial qui reste, pour l'homme, un antidote à toutes les formes d'organisation qui le dépassent.

  19. Cardiotoxicité des psychotropes

    OpenAIRE

    TAHIRI, Abdallah

    2013-01-01

    Même à dose thérapeutique, les médicaments psychotropes sont susceptibles d'engendrer des troubles du rythme cardiaque graves avec risque létal concourant à expliquer la pré valence de la mort subite dans la population psychiatrique. Les situations cliniques à risque telles que poly médication (des psychotropes entre eux ou d'un psychotrope avec un non psychotrope allongeur de QTc), interactions médicamenteuses aussi bien pharmacodynamiques que pharmacocinétiques, traitement pa...

  20. Physique statistique des Fluides Classiques

    OpenAIRE

    Aslangul, Claude

    2006-01-01

    0 - Préambule.Préambule, table des matières1 - Rappels sur la description d'un système à l'équilibre thermodynamiqueQuelques propriétés des systèmes macroscopiques. Nature statistique de l'entropie. Grandeurs internes et grandeurs externes. Relations thermodynamiques fondamentales. Potentiels thermodynamiques. Ensembles microcanonique, canonique, grand-canonique et isotherme - isobare. Exemple: fonction de partition d'un fluide classique. Principe variationnel pour l'énergie libre.2 - Stabili...

  1. La maison des mathématiques

    CERN Document Server

    Villani, Cédric; Moncorgé, Vincent

    2014-01-01

    Comment travaillent les mathématiciens ? C'est peut-être en se promenant dans les couloirs de la première des " maisons des mathématiques " de France, l'institut Henri Poincaré, que l'on trouvera quelques réponses. Le mathématicien Cédric Villani et le physicien Jean-Philippe Uzan nous invitent à découvrir cette discipline et ses acteurs. Au fil des pages on suit, à travers de superbes images signées du photographe Vincent Moncorgé, la façon dont se fabrique cette science qui reste souvent mystérieuse. Toutes les dimensions, scientifique, esthétique et poétique, des mathématiques sont convoquées grâce à des regards croisés : la diversité des inspirations des chercheurs, la source de leur créativité, l'imaginaire littéraire et artistique des mathématiques, la drôle de tribu des mathématiciens. Un voyage au cœur de cette " auberge espagnole " des mathématiques, campus " à la française " accueillant des centaines de chercheurs du monde entier, devenu un lieu d'émulation et d'éc...

  2. Diseño de redes neuronales con aprendizaje combinado de retropropagación y búsqueda aleatoria progresiva aplicado a la determinación de austenita retenida en aceros TRIP

    Directory of Open Access Journals (Sweden)

    Capdevila, C.

    2010-12-01

    Full Text Available At the beginning of the decade of the nineties, the industrial interest for TRIP steels leads to a significant increase of the investigation and application in this field. In this work, the flexibility of neural networks for the modelling of complex properties is used to tackle the problem of determining the retained austenite content in TRIP-steel. Applying a combination of two learning algorithms (backpropagation and creeping-random-search for the neural network, a model has been created that enables the prediction of retained austenite in low-Si / low-Al multiphase steels as a function of processing parameters.

    A partir de los años noventa, el interés que los aceros TRIP despiertan en la industria ha producido un incremento considerable de su estudio y aplicación. En este trabajo, aprovechando la flexibilidad que las redes neuronales proporcionan para la modelización de propiedades complejas, se ha abordado el problema de la determinación de la austenita retenida en los aceros TRIP. Una combinación de dos algoritmos de aprendizaje (retropropagación y búsqueda aleatoria progresiva de la red neuronal ha permitido crear un modelo que predice la cantidad de austenita retenida en aceros multifase con bajo contenido en aluminio y silicio en función de los parámetros de procesado.

  3. Diagnostico de Fallas en Motores de Inducción Mediante la Aplicación de Redes Neuronales Artificiales Fault Diagnosis in Induction Motors Using Artificial Neuronal Networks

    Directory of Open Access Journals (Sweden)

    Fernando Villada

    2007-01-01

    Full Text Available En este trabajo se presenta un algoritmo para diagnosticar fallas entre espiras del estator de motores de inducción mediante la aplicación de redes neuronales artificiales (RNA. Los patrones de entrenamiento de las RNA son obtenidos a partir de un modelo de máquina que permite simular fallas internas bajo diferentes condiciones de carga y desequilibrio de tensión. Se muestra la implementación del método utilizando un analizador de redes eléctricas y un procesador digital de señales (DSP. Los resultados obtenidos experimentalmente en dos motores de 2 Hp y 3 HP permiten concluir la fortaleza del algoritmo al permitir detectar fallas incipientes en motores de inducción y la factibilidad de implementación del mismo a nivel industrial.A new algorithm to diagnose inter-turn faults in induction motors based on Artificial Neural Networks (ANN is presented in this work. A machine model able to simulate internal faults under different load conditions and voltage unbalance was implemented and tested, in order to generate the training patterns of the ANN. An electrical network analyzer and a digital signal processor (DSP are used to show the implementation of the method. Experimental results in a 2 Hp and 3 Hp induction motors show the robustness of the algorithm allowing detect incipient faults and its implementation feasibility at industrial plants.

  4. An application of neural network in geophysical prospecting. Electrical resistivity at Las Virgenes geothermal field, Baja California Sur, Mexico; Una aplicacion de las redes neuronales a la prospeccion geofisica. Resistividad electrica en las Tres Virgenes, Baja California Sur, Mexico

    Energy Technology Data Exchange (ETDEWEB)

    Palma Guzman, Sergio Hugo [Comision Federal de Electricidad, Morelia, Michoacan (Mexico)

    2000-12-01

    The technology of the neural network is presented with geophysical focus in the Las Virgenes geothermal field, Baja California Sur, Mexico. The results obtained when extrapolating the associative data of the prospecting magnetoteluria and Vertical Electric Sounding, on the area of the geothermal wells to the rest of the area, allows to classify zones of interest for the geothermal exploitation. Also, the use of these associative parameters with the information of the stabilized temperature of the wells, they allow to predict temperatures for the rest of the area. [Spanish] Se presenta una aplicacion de la tecnologia de las redes neuronales con enfoque geofisico en el campo geotermico de Las Virgenes, Baja California Sur, Mexico. Los resultados obtenidos al extrapolar los datos asociativos de las prospecciones geoelectricas de magnetoteluria y sondeos electricos verticales, en la zona de los pozos geotermicos al resto del area, permiten clasificar zonas de interes para la explotacion geotermica. Tambien, la utilizacion de estos parametros asociativos con la informacion de la temperatura estabilizada de los pozos, permiten predecir temperaturas para la misma area.

  5. Prediction of the daily share price fluctuations of SURAMINV. A neural netword model Predicción del comportamiento diario de la acción de SURAMINV. Un modelo de redes neuronales

    Directory of Open Access Journals (Sweden)

    Jaime Enrique Arrieta Bechara

    2009-12-01

    Full Text Available As opposed to the weak form of efficient-market hypothesis, the current study shows that it is possible to do good predictions about the daily share price fluctuations of Suraminv, using artificial neural network models. Furthermore, the forecasts obtained are used to analyze the possibility of gaining extraordinary returns with regard to the Buy & Hold strategy, through negotiation systems with basic rules.La investigación muestra que es posible realizar, por medio de modelos de redes neuronales artificiales, buenas predicciones sobre el comportamiento diario de la acción de SURAMINV. Tales resultados contrarían la hipótesis de la teoría de eficiencia débil de mercado. A partir de dichas predicciones y con el uso de sistemas de negociación, se evalúa la posibilidad de obtener rendimientos extraordinarios sobre la estrategia Buy & Hold teniendo en cuenta costos de transacción y oportunidad.

  6. La compaction des sols forestiers en Wallonie

    OpenAIRE

    Destain, Marie-France

    2014-01-01

    Dans nos pays industrialisés, l’exploitation forestière fait appel à des machines de plus en plus lourdes pour assurer la vidange des coupes et le débardage des grumes. Lorsque des charges élevées sont appliquées sur des sols sensibles, une compaction du sol peut se produire entraînant une réduction de porosité, avec des conséquences néfastes sur la vitalité des peuplements. Au niveau environnemental, du fait de la compaction, l’infiltration de l’eau dans le sol est réduite, principalement...

  7. Etude des erreurs d'estimation des populations par la méthode des captures successives (DeLURY, 2 captures et des captures-recaptures (PETERSEN

    Directory of Open Access Journals (Sweden)

    LAURENT M.

    1978-01-01

    Full Text Available L'estimation des populations naturelles par capture-recapture et par captures successives est souvent entachée d'erreur car, dans de nombreux cas, l'hypothèse fondamentale d'égalité des probabilités de captures pour tous les individus dans le temps et dans l'espace n'est pas respectée. Dans le cas des populations de poissons envisagés ici, les captures ont lieu par la pêche électrique. On a pu chiffrer l'ordre de grandeur des erreurs systématiques faites sur l'estimation des peuplements, en fonction des conditions particulières, biotiques et abiotiques, des différents milieux inventoriés.

  8. Recherche des oscillations de Neutrinos $\

    CERN Document Server

    Gangler, E

    1997-01-01

    Le detecteur nomad, place sur le faisceau de neutrinos wide-band-beam du sps, de contamination en neutrino tau marginale, permet de rechercher des oscillations neutrino muon - tau dans la region de pertinence cosmologique et de distinguer statistiquement les courants charges des neutrinos tau essentiellement par leur mesure cinematique. Une large part du travail de these a donc ete consacree a la reconstruction des evenements dans les chambres a derive, cible instrumentee et cur de l'experience, dont la physique de detection est decrite. Une methode de recherche de traces fut developpee, utilisant certaines informations d'un autre sous-detecteur de nomad, le trd. Pour combler une perte d'efficacite de reconstruction, une methode de recherche de traces courtes s'appuyant sur des vertex deja constitues fut developpee en exploitant les potentialites du filtre de kalman, algorithme iteratif d'ajustement de traces. Ces methodes sont utilisees en production par la collaboration. Cette these porte sur la recherche d...

  9. Reduction des effectifs ou licenciements

    CERN Multimedia

    Maiani, Luciano

    2002-01-01

    "Vous faites un amalgame entre la reduction en cours des effectifs du CERN (organisation europeenne pour la recherche nucleaire) et les economies que le laboratoire doit realiser dans les cinq ans a venir pour financer le projet de grand collisionneur de hadrons (Le Monde du 4 septembre)" (1/2 page).

  10. Bangalore, ville des nouvelles technologies

    Directory of Open Access Journals (Sweden)

    Clarisse Didelon

    2003-06-01

    Full Text Available Bangalore est devenue la Silicon Valley de l’Inde. Une partie de ses habitants y vit à l’occidentale mais le reste de la population souffre de la croissance spectaculaire de la ville. Face à l’insuffisance des infrastructures, Bangalore devient de moins en moins attractive pour les entreprises internationales.

  11. Osteoporose und Genetik des Knochenstoffwechsels

    Directory of Open Access Journals (Sweden)

    Obermayer-Pietsch B

    2002-01-01

    Full Text Available Osteoporose ist in hohem Maß genetisch determiniert. Neue Wege der molekularbiologischen Forschung haben sich in den letzten Jahren auf diesem Gebiet etabliert. "Gene mapping" mit polymorphen genetischen Markern auf der Suche nach Phänotyp-assoziierten Genen ist ein aufwendiges, aber vielversprechendes Verfahren und wird durch die Erkenntnisse des Human Genome Projects beschleunigt. So wurde jüngst u. a. das Low-density Lipoprotein 5-Gen als wichtig für den Knochenstoffwechsel identifiziert. Kandidaten-gene wie Hormonrezeptor-, Cytokin- oder Kollagen-Gene werden hinsichtlich ihrer Gen-Gen- und Gen-Umwelt- Interaktionen untersucht und erlauben neue funktionelle Einsichten in Erkrankungen des Knochenstoffwechsels. Mutationen der Kollagen-Gene sind bei einigen seltenen Erkrankungen, wie dem Osteoporose-Pseudogliom-Syndrom oder der Osteogenesis imperfecta gefunden worden, könnten aber auch für häufige Varianten von Bindegewebsstörungen wie der congenitalen Hüftdysplasie verantwortlich sein, die etwa 10 % der weiblichen kaukasischen Bevölkerung in unterschiedlichem Ausmaß betrifft. Osteoporose am Schenkelhals und erhöhte Gelenkslaxizität sowie andere generalisierte Veränderungen des Knochen- und Kollagenstoffwechsels können hier möglicherweise ebenfalls durch Störungen des Kollagen I alpha 1-Gens erklärt werden. In Summe können wir zahlreiche neue Einsichten in die Pathophysiologie des Skelettsystems erwarten, die uns auch neue Zugangswege für Diagnostik und Therapie unserer Patienten ermöglichen werden.

  12. L'astronomie des Anciens

    Science.gov (United States)

    Nazé, Yaël

    2009-04-01

    Quelle que soit la civilisation à laquelle il appartient, l'être humain cherche dans le ciel des réponses aux questions qu'il se pose sur son origine, son avenir et sa finalité. Le premier mérite de ce livre est de nous rappeler que l'astronomie a commencé ainsi à travers les mythes célestes imaginés par les Anciens pour expliquer l'ordre du monde et la place qu'ils y occupaient. Mais les savoirs astronomiques passés étaient loin d'être négligeables et certainement pas limités aux seuls travaux des Grecs : c'est ce que l'auteur montre à travers une passionnante enquête, de Stonehenge à Gizeh en passant par Pékin et Mexico, fondée sur l'étude des monuments anciens et des sources écrites encore accessibles. Les tablettes mésopotamiennes, les annales chinoises, les chroniques médiévales, etc. sont en outre d'une singulière utilité pour les astronomes modernes : comment sinon remonter aux variations de la durée du jour au cours des siècles, ou percer la nature de l'explosion qui a frappé tant d'observateurs en 1054 ? Ce livre offre un voyage magnifiquement illustré à travers les âges, entre astronomie et archéologie.

  13. Tambov. Le camp des Malgré Nous alsaciens et mosellans prisonniers des Russes

    OpenAIRE

    Claerr-Stamm, Gabrielle

    2012-01-01

    « La faim, le froid, l’injustice. Et puis le silence… Tambov égale silence. Silence de la grande majorité de ceux qui en sont revenus, silence dans les familles, silence des historiens, silence des archives, silence des écrivains et des artistes, silence de l’opinion publique ». « Il a fallu l’opiniâtre courage de poignées d’individus – anciens prisonniers, responsables d’associations, quelques élus, des historiens et des journalistes – pour que la parole et la reconnaissance trouvent progres...

  14. Vers une typologie des formes spatiales des limites de l’Europe

    OpenAIRE

    de Ruffray, Sophie

    2013-01-01

    Penser l’Europe et ses limites consiste souvent à rechercher une identité européenne, un projet ou des comportements communs dans l’espace. L’approche par les représentations mentales permet de compléter par des perspectives plus subjectives, la perception des frontières. Cet article, réalisé à partir des résultats de l’enquête du projet de recherches Eurobroadmap sur la vision de l’Europe dans le monde permet de mettre en évidence une typologie des formes spatiales des limites de l’Europe. D...

  15. Administration et gestion des contrats XL

    CERN Document Server

    Senouf, J

    2000-01-01

    Les grands contrats de travaux génèrent un grand nombre de commandes (OSVC) passées par un grand nombre de responsables techniques. Ils sont généralement basés sur des bordereaux de prix associés à un éventail de conditions économiques adaptées aux besoins complexes et variés des multiples utilisateurs du CERN nécessitant des calculs sophistiqués. L'administration des commandes, le suivi des travaux et de la facturation doivent répondre aux besoins de tous les acteurs. Les responsables techniques doivent jouir de la plus large indépendance administrative dans le respect des règlements CERN et des conditions contractuelles. Le contrôle des métrés et décomptes doit être rigoureux et d'une traçabilité complète. L'utilisation des bases de données Oracle a déjà permis l'intégration des données administratives et techniques. Le Web nous invite à une communication totale et transparente entre les utilisateurs, les services techniques et les contractants. De nouveaux types de contrat so...

  16. Enquête de satisfaction des restaurants

    CERN Multimedia

    Staff Association

    2016-01-01

    Comité de surveillance des restaurants L’Association du personnel est représentée dans plusieurs comités dont le Comité de surveillance des restaurants (CSR) qui a pour mandat : de donner son avis sur toute question relative à la politique générale de l’Organisation en matière de restauration sur le site, y compris en ce qui concerne les termes et l’attribution des contrats d’exploitation des restaurants ; de définir dans le cadre des contrats d’exploitation des restaurants, les prestations correspondant aux besoins et, dans la mesure du possible, aux désirs du personnel ; de surveiller les prestations des restaurants, y compris en ce qui concerne la qualité et la préparation des produits ; de négocier avec les concessionnaires des restaurants au sujet des tarifs et de surveill...

  17. PSA Isoforms' Velocities for Early Diagnosis of Prostate Cancer.

    Science.gov (United States)

    Heidegger, Isabel; Klocker, Helmut; Pichler, Renate; Horninger, Wolfgang; Bektic, Jasmin

    2015-06-01

    Free prostate-specific antigen (fPSA) and its molecular isoforms are suggested for enhancement of PSA testing in prostate cancer (PCa). In the present study we evaluated whether PSA isoforms' velocities might serve as a tool to improve early PCa diagnosis. Our study population included 381 men who had undergone at least one ultrasound-guided prostate biopsy whose pathologic examination yielded PCa or showed no evidence of prostatic malignancy. Serial PSA, fPSA, and proPSA measurements were performed on serum samples covering 7 years prior to biopsy using Beckmann Coulter Access immunoassays. Afterwards, velocities of PSA (PSAV), fPSA% (fPSA%V), proPSA% (proPSA%V) and the ratio proPSA/PSA/V were calculated and their ability to discriminate cancer from benign disease was evaluated. Among 381 men included in the study, 202 (53%) were diagnosed with PCa and underwent radical prostatectomy at our Department. PSAV, fPSA%V, proPSA%V as well as proPSA/PSA/V were able to differentiate significantly between PCa and non-cancerous prostate. The highest discriminatory power between cancer and benign disease has been observed two and one year prior to diagnosis with all measured parameters. Among all measured parameters, fPSA%V showed the best cancer specificity of 45.3% with 90% of sensitivity. In summary, our results highlight the value of PSA isoforms' velocity for early detection of PCa. Especially fPSA%V should be used in the clinical setting to increase cancer detection specificity.

  18. Identification of an alternative splicing isoform of chicken Lmbr1.

    Science.gov (United States)

    Huang, Yanqun; Chen, Wen; Li, Ning; Deng, Xuemei; Kang, Xiangtao; Liu, Xiaojun

    2011-10-01

    Lmbr1 is the key candidate gene for limb development. Until now, at least five and four alternative splicing isoforms of Lmbr1 gene have been found in human and mouse, respectively. However, only two alternative splicing isoforms of this homologous gene have been reported in chicken. In the present study, one novel chicken Lmbr1 transcript variant (designated Lmbr1-1) was identified by 5' RACE and RT-PCR. Chicken Lmbr1-1 possesses one novel transcription start site different from Lmbr1-N, and was predicted to encode one 192 amino acid protein with length variation in comparison with chicken LMBR1-N protein, which was produced by 5' spliced site variation of chicken Lmbr1-N exon 10. Comparing with Lmbr1-N transcript, chicken Lmbr1-1 exhibited restricted tissue distribution of the expression. Comparative sequence analysis revealed a highly conservative intron element between chicken and mammalians from the intron 9 of chicken Lmbr1-N, indicating their possible importance as intronic elements in the regulation of alternative splicing of Lmbr1 in vertebrates. By direct PCR sequencing the exon 10 and its flanking sequences in chicken Lmbr1-N, four variation sites/haplotypes were identified from six chicken breeds. One 797A/G nonsynonymous mutation (266Arg/Gln) locating in exon 10 of chicken Lmbr1-N was predicted to affect the exon splice enhancer motif for serine/arginine-rich protein recognition. These data demonstrated that chicken Lmbr1 was alternatively spliced to generate multiple splice forms, as was the case in mammals and each of the alternative splicing isoforms might function differentially.

  19. The role of wild type RAS isoforms in cancer.

    Science.gov (United States)

    Zhou, Bingying; Der, Channing J; Cox, Adrienne D

    2016-10-01

    Mutationally activated RAS proteins are critical oncogenic drivers in nearly 30% of all human cancers. As with mutant RAS, the role of wild type RAS proteins in oncogenesis, tumour maintenance and metastasis is context-dependent. Complexity is introduced by the existence of multiple RAS genes (HRAS, KRAS, NRAS) and protein "isoforms" (KRAS4A, KRAS4B), by the ever more complicated network of RAS signaling, and by the increasing identification of numerous genetic aberrations in cancers that do and do not harbour mutant RAS. Numerous mouse model carcinogenesis studies and examination of patient tumours reveal that, in RAS-mutant cancers, wild type RAS proteins are likely to serve as tumour suppressors when the mutant RAS is of the same isoform. This evidence is particularly robust in KRAS mutant cancers, which often display suppression or loss of wild type KRAS, but is not as strong for NRAS. In contrast, although not yet fully elucidated, the preponderance of evidence indicates that wild type RAS proteins play a tumour promoting role when the mutant RAS is of a different isoform. In non-RAS mutant cancers, wild type RAS is recognized as a mediator of oncogenic signaling due to chronic activation of upstream receptor tyrosine kinases that feed through RAS. Additionally, in the absence of mutant RAS, activation of wild type RAS may drive cancer upon the loss of negative RAS regulators such as NF1 GAP or SPRY proteins. Here we explore the current state of knowledge with respect to the roles of wild type RAS proteins in human cancers.

  20. Modulation of Progesterone Receptor Isoform Expression in Pregnant Human Myometrium

    Directory of Open Access Journals (Sweden)

    Marina Ilicic

    2017-01-01

    Full Text Available Background. Regulation of myometrial progesterone receptor (PR expression is an unresolved issue central to understanding the mechanism of functional progesterone withdrawal and initiation of labor in women. Objectives. To determine whether pregnant human myometrium undergoes culture-induced changes in PR isoform expression ex situ and, further, to determine if conditions approaching the in vivo environment stabilise PR isoform expression in culture. Methods. Term nonlaboring human myometrial tissues were cultured under specific conditions: serum supplementation, steroids, stretch, cAMP, PMA, PGF2α, NF-κB inhibitors, or TSA. Following 48 h culture, PR-T, PR-A, and PR-B mRNA levels were determined using qRT-PCR. PR-A/PR-B ratios were calculated. Results. PR-T and PR-A expression and the PR-A/PR-B ratio significantly increased in culture. Steroids prevented the culture-induced increase in PR-T and PR-A expression. Stretch blocked the effects of steroids on PR-T and PR-A expression. PMA further increased the PR-A/PR-B ratio, while TSA blocked culture-induced increases of PR-A expression and the PR-A/PR-B ratio. Conclusion. Human myometrial tissue in culture undergoes changes in PR gene expression consistent with transition toward a laboring phenotype. TSA maintained the nonlaboring PR isoform expression pattern. This suggests that preserving histone and/or nonhistone protein acetylation is critical for maintaining the progesterone dependent quiescent phenotype of human myometrium in culture.

  1. Modulation of Progesterone Receptor Isoform Expression in Pregnant Human Myometrium

    Science.gov (United States)

    2017-01-01

    Background. Regulation of myometrial progesterone receptor (PR) expression is an unresolved issue central to understanding the mechanism of functional progesterone withdrawal and initiation of labor in women. Objectives. To determine whether pregnant human myometrium undergoes culture-induced changes in PR isoform expression ex situ and, further, to determine if conditions approaching the in vivo environment stabilise PR isoform expression in culture. Methods. Term nonlaboring human myometrial tissues were cultured under specific conditions: serum supplementation, steroids, stretch, cAMP, PMA, PGF2α, NF-κB inhibitors, or TSA. Following 48 h culture, PR-T, PR-A, and PR-B mRNA levels were determined using qRT-PCR. PR-A/PR-B ratios were calculated. Results. PR-T and PR-A expression and the PR-A/PR-B ratio significantly increased in culture. Steroids prevented the culture-induced increase in PR-T and PR-A expression. Stretch blocked the effects of steroids on PR-T and PR-A expression. PMA further increased the PR-A/PR-B ratio, while TSA blocked culture-induced increases of PR-A expression and the PR-A/PR-B ratio. Conclusion. Human myometrial tissue in culture undergoes changes in PR gene expression consistent with transition toward a laboring phenotype. TSA maintained the nonlaboring PR isoform expression pattern. This suggests that preserving histone and/or nonhistone protein acetylation is critical for maintaining the progesterone dependent quiescent phenotype of human myometrium in culture. PMID:28540297

  2. PKC isoforms interact with and phosphorylate DNMT1

    Directory of Open Access Journals (Sweden)

    Pradhan Sriharsa

    2011-05-01

    Full Text Available Abstract Background DNA methyltransferase 1 (DNMT1 has been shown to be phosphorylated on multiple serine and threonine residues, based on cell type and physiological conditions. Although recent studies have suggested that protein kinase C (PKC may be involved, the individual contribution of PKC isoforms in their ability to phosphorylate DNMT1 remains unknown. The PKC family consists of at least 12 isoforms that possess distinct differences in structure, substrate requirement, expression and localization. Results Here we show that PKCα, βI, βII, δ, γ, η, ζ and μ preferentially phosphorylate the N-terminal domain of human DNMT1. No such phosphorylation of DNMT1 was observed with PKCε. Using PKCζ as a prototype model, we also found that PKC physically interacts with and phosphorylates DNMT1. In vitro phosphorylation assays conducted with recombinant fragments of DNMT1 showed that PKCζ preferentially phosphorylated the N-terminal region of DNMT1. The interaction of PKCζ with DNMT1 was confirmed by GST pull-down and co-immunoprecipitation experiments. Co-localization experiments by fluorescent microscopy further showed that endogenous PKCζ and DNMT1 were present in the same molecular complex. Endogenous PKCζ activity was also detected when DNMT1 was immunoprecipitated from HEK-293 cells. Overexpression of both PKCζ and DNMT1 in HEK-293 cells, but not of either alone, reduced the methylation status of genes distributed across the genome. Moreover, in vitro phosphorylation of DNMT1 by PKCζ reduced its methytransferase activity. Conclusions Our results indicate that phosphorylation of human DNMT1 by PKC is isoform-specific and provides the first evidence of cooperation between PKCζ and DNMT1 in the control of the DNA methylation patterns of the genome.

  3. La territorialisation des politiques environnementales

    Directory of Open Access Journals (Sweden)

    Amédée Mollard

    2010-10-01

    Full Text Available Le point de départ de cet article est la grande variabilité de la pollution nitrique diffuse de l’eau due à l’agriculture. Celle-ci dépend en particulier du climat, des types de sol et des systèmes de production agricole. Nos recherches réalisées de façon interdisciplinaire à partir de deux sites différents en France, montrent que cette hétérogénéité spatio-temporelle conditionne les pratiques agricoles mises en œuvre pour réduire la pollution au niveau de la norme admise. De ce fait, les pratiques les plus « coût-efficaces » diffèrent d’un territoire à l’autre, en fonction des caractéristiques locales. Ces résultats mettent donc en évidence une efficacité potentielle d’une territorialisation des politiques publiques. En théorie, de telles politiques sont considérées comme optimales par les économistes, car elles incitent les agents à moduler leurs efforts en fonction de la sensibilité du milieu. Mais, selon les études empiriques, cet avantage serait annulé par un coût élevé de mise en œuvre, de contrôle et de surveillance. Pour maintenir leur avantage sur des politiques uniformes, les politiques territorialisées devraient être mises en œuvre à un niveau spatial optimal. Un tel niveau devrait au minimum atteindre un compromis entre l’économie réalisée grâce à une modulation adaptée aux conditions locales et les sur-coûts dus à la décentralisation des solutions mises en œuvre. Cet article analyse la pertinence d’une prise en compte de ces spécificités par des politiques territorialisées. L’efficacité d’une régulation différenciée de la pollution nitrique est étudiée ici en évaluant l’importance de la variabilité spatiale des paramètres physiques et des coûts de la territorialisation.The paper starts with the wide variability of nonpoint water nitrogenous pollution generated by agriculture. This variability depends especially on climate, soil types and farming

  4. The FU gene and its possible protein isoforms

    Directory of Open Access Journals (Sweden)

    Nöthen Markus M

    2004-07-01

    Full Text Available Abstract Background FU is the human homologue of the Drosophila gene fused whose product fused is a positive regulator of the transcription factor Cubitus interruptus (Ci. Thus, FU may act as a regulator of the human counterparts of Ci, the GLI transcription factors. Since Ci and GLI are targets of Hedgehog signaling in development and morphogenesis, it is expected that FU plays an important role in Sonic, Desert and/or Indian Hedgehog induced cellular signaling. Results The FU gene was identified on chromosome 2q35 at 217.56 Mb and its exon-intron organization determined. The human developmental disorder Syndactyly type 1 (SD1 maps to this region on chromosome 2 and the FU coding region was sequenced using genomic DNA from an affected individual in a linked family. While no FU mutations were found, three single nucleotide polymorphisms were identified. The expression pattern of FU was thoroughly investigated and all examined tissues express FU. It is also clear that different tissues express transcripts of different sizes and some tissues express more than one transcript. By means of nested PCR of specific regions in RT/PCR generated cDNA, it was possible to verify two alternative splicing events. This also suggests the existence of at least two additional protein isoforms besides the FU protein that has previously been described. This long FU and a much shorter isoform were compared for the ability to regulate GLI1 and GLI2. None of the FU isoforms showed any effects on GLI1 induced transcription but the long form can enhance GLI2 activity. Apparently FU did not have any effect on SUFU induced inhibition of GLI. Conclusions The FU gene and its genomic structure was identified. FU is a candidate gene for SD1, but we have not identified a pathogenic mutation in the FU coding region in a family with SD1. The sequence information and expression analyses show that transcripts of different sizes are expressed and subjected to alternative splicing

  5. Evaluation des systèmes existants pour le suivi à long terme des déformations des ponts

    OpenAIRE

    BURDET, Olivier; Muttoni, Aurelio

    2006-01-01

    Le projet de recherche Evaluation des systèmes existants pour le suivi à long terme des déformations des ponts a été initié dans le but de préparer sous forme condensée une présentation et une évaluation des systèmes de mesure existants pour le suivi à long terme des déformations de ponts ainsi que des recommandations en vue d’applications particulières. Ce sujet est d’actualité car d’une part il importe de gérer au mieux les ouvrages et les ressources et d’autre part parce que plusieurs cas ...

  6. Géographie des interfaces. Une nouvelle vision des territoires

    Directory of Open Access Journals (Sweden)

    Marie Redon

    2011-07-01

    Full Text Available Littéralement, une interface est une surface de séparation entre deux états distincts de la matière. Le terme, ayant cheminé de la physique à la biologie, et aussi désormais d’usage répandu en informatique. En géographie, l’usage du mot s’est développé dans les années 1980 et peut être défini comme un espace permettant la mise en relation de deux espaces/territoires différents, influencé par des échanges entre l’un et l’autre, et se distinguant par là-même des deux espaces contigus. Intégrant...

  7. Evaluation des performances des protocoles de routage Ad hoc

    OpenAIRE

    Boushaba, Abdelali; Oumsis, Mohammed; Benabbou, Rachid

    2010-01-01

    International audience; L'objectif de ce travail est d'une part, de confronter par la simulation, à l'aide de NS-2, les performances de quatre protocoles de routage Ad hoc: DSR, AODV, OLSR et DSDV et d'autre part, d'examiner l'impact de la charge du trafic, de la mobilité et de la densité des nœuds sur le comportement de ces protocoles. Les résultats montrent qu'il n'y a pas un protocole qui est favori pour tous les critères d'évaluation. En effet, chaque protocole a des comportements différe...

  8. Soluble malate dehydrogenase of Geophagus brasiliensis (Cichlidae, Perciformes: isolated isoforms and kinetics properties

    Directory of Open Access Journals (Sweden)

    Maria Regina de Aquino-Silva

    2008-01-01

    Full Text Available Kinetic properties and thermal stabilities of Geophagus brasiliensis skeletal muscle unfractionated malate dehydrogenase (MDH, EC 1.1.1.37 and its isolated isoforms were analyzed to examine a possible sMDH-B* locus duplication in a fixation process influenced by genetic drift. Two optimal pHs were detected: 7.5 for AB1 unfractionated muscle phenotype and its B1 isoform, and 8.0 for AB1B2 unfractionated muscle phenotype, A and B2 isoforms. While G. brasiliensis A isoform could be characterized as thermostable, the duplicated B isoform cannot be assumed as thermolabile. Km values for isolated B2 isoforms were 1.6 times lower than for B1. A duplication event in progress best explains the electrophoretic six-band pattern detected in G. brasiliensis, which would be caused by genetic drift.

  9. Isoform-specific regulation of adenylyl cyclase: a potential target in future pharmacotherapy.

    Science.gov (United States)

    Iwatsubo, Kousaku; Tsunematsu, Takashi; Ishikawa, Yoshihiro

    2003-06-01

    Adenylyl cyclase (AC) is a target enzyme of multiple G-protein-coupled receptors (GPCRs). In the past decade, the cloning, structure and biochemical properties of nine AC isoforms were reported, and each isoform of AC shows distinct patterns of tissue distribution and biochemical/pharmacological properties. In addition to the conventional regulators of this enzyme, such as calmodulin (CaM) or PKC, novel regulators, for example, caveolin, have been identified. Most importantly, these regulators work on AC in an isoform dependent manner. Recent studies have demonstrated that certain classic AC inhibitors, i.e., P-site inhibitors, show an isoform-dependent inhibition of AC. The side chain modifications of forskolin, a diterpene extract from Coleus forskolii, markedly enhance its isoform selectivity. When taken together, these findings suggest that it is feasible to develop new pharmacotherapeutic agents that target AC isoforms to regulate various neurohormonal signals in a highly tissue-/organ-specific manner.

  10. Separation of arginase isoforms by capillary zone electrophoresis and isoelectric focusing in density gradient column.

    Science.gov (United States)

    Pedrosa, M M; Legaz, M E

    1995-04-01

    Four major arginase isoforms, I, II, III and IV, have been detected in Evernia prunastri thallus. They differ in terms of both physical and biochemical properties. The isoelectric point (pI) of these proteins has been determined by both isoelectric focusing in density gradient column and high-performance capillary electrophoresis (HPCE). Isoelectric focusing revealed charge microheterogeneity for isoforms II and IV whereas arginases I and II had the same pI value of 5.8. HPCE separation confirmed this charge microheterogeneity for isoform IV but not for isoform III, and provided evidence of microheterogeneity for isoforms I and II. The effect of various electrolyte buffers and running conditions on the HPCE separation of arginase isoform were investigated. Addition of 0.5 mM spermidine (SPD) to the running buffer reduced the electroosmotic flow (EOF) and permitted discriminating between the native proteins and protein fragments.

  11. Marie-Pierre Lassus, Gaston Bachelard musicien. Une philosophie des silences et des timbres

    OpenAIRE

    Marida Rizzuti

    2012-01-01

    Marie-Pierre Lassus, Gaston Bachelard musicien. Une philosophie des silences et des timbres (Villeneuve d’Ascq, Presses Universitaires du Septentrion, 2010, 268 pp. ISBN: 978-275-740-1613) di     Marida Rizzuti

  12. CD44 variant isoforms in non-Hodgkin's lymphoma : a new independent prognostic factor

    OpenAIRE

    Stauder, R.; Eisterer, W.; Thaler, J; Günthert, U

    1995-01-01

    Isoforms of the transmembrane glycoprotein CD44, generated by alternative RNA splicing, have been correlated to tumor dissemination. For evaluation of the potential role of CD44 variant isoforms in non-Hodgkin's lymphoma (NHL), the presence of CD44 isoforms was analyzed in a large panel of reactive and neoplastic lymphoid tissues by immunohistochemical staining, as well as detection of CD44 variant RNAs by the reverse transcriptase-polymerase chain reaction. Whereas the CD44 standard or hemat...

  13. Identification of signals that facilitate isoform specific nucleolar localization of myosin IC

    Energy Technology Data Exchange (ETDEWEB)

    Schwab, Ryan S.; Ihnatovych, Ivanna; Yunus, Sharifah Z.S.A.; Domaradzki, Tera [Department of Physiology and Biophysics, University at Buffalo—State University of New York, Buffalo, NY (United States); Hofmann, Wilma A., E-mail: whofmann@buffalo.edu [Department of Physiology and Biophysics, University at Buffalo—State University of New York, Buffalo, NY (United States)

    2013-05-01

    Myosin IC is a single headed member of the myosin superfamily that localizes to the cytoplasm and the nucleus, where it is involved in transcription by RNA polymerases I and II, intranuclear transport, and nuclear export. In mammalian cells, three isoforms of myosin IC are expressed that differ only in the addition of short isoform-specific N-terminal peptides. Despite the high sequence homology, the isoforms show differences in cellular distribution, in localization to nuclear substructures, and in their interaction with nuclear proteins through yet unknown mechanisms. In this study, we used EGFP-fusion constructs that express truncated or mutated versions of myosin IC isoforms to detect regions that are involved in isoform-specific localization. We identified two nucleolar localization signals (NoLS). One NoLS is located in the myosin IC isoform B specific N-terminal peptide, the second NoLS is located upstream of the neck region within the head domain. We demonstrate that both NoLS are functional and necessary for nucleolar localization of specifically myosin IC isoform B. Our data provide a first mechanistic explanation for the observed functional differences between the myosin IC isoforms and are an important step toward our understanding of the underlying mechanisms that regulate the various and distinct functions of myosin IC isoforms. - Highlights: ► Two NoLS have been identified in the myosin IC isoform B sequence. ► Both NoLS are necessary for myosin IC isoform B specific nucleolar localization. ► First mechanistic explanation of functional differences between the isoforms.

  14. Amyloid-beta Isoform Metabolism Quantitation by Stable Isotope Labeled Kinetics

    OpenAIRE

    Mawuenyega, Kwasi G.; Kasten, Tom; Sigurdson, Wendy; Bateman, Randall J.

    2013-01-01

    Abundant evidence suggests a central role for the amyloid-β (Aβ) peptide in Alzheimer’s disease (AD) pathogenesis. Production and clearance of different Aβ isoforms have been established as targets of proposed disease-modifying therapeutic treatments of AD. However, previous studies used multiple sequential purification steps to isolate the isoforms individually and quantitate them based on a common mid-domain peptide. We created a method to simultaneously purify Aβ isoforms and quantitate th...

  15. The Role of a Novel Myosin Isoform in Prostate Cancer Metastasis

    Science.gov (United States)

    2013-10-01

    specific N-terminal peptides. Despite the high sequence homology , the isoforms show differences in cellular distribution, in localization to nuclear...Interestingly, despite the sequence homology between the isoforms and the commonality of the NLS, the isoforms show differences in their nucleus to cytoplasm...Goeres J, Sixt KM, Bekes M, Zhang XD, Salvesen GS, Matunis MJ (2009). Protection from isopeptidase-mediated deconjugation regulates paralog -selective

  16. Metodología AED-RNA para la estimación de la eficiencia institucional: El caso de las dependencias de educación superior (DES de ingeniería de México

    Directory of Open Access Journals (Sweden)

    Antonio Altamirano-Corro

    2014-01-01

    Full Text Available Introducción: En la presente investigación se discute la utilización del Análisis Envolvente de Datos (AED combinado con Redes Neuronales Artificiales (RNA para la evaluación de la eficiencia institucional, en este caso de las Dependencias de Educación Superior (DES de Ingeniería en México. Ambas metodologías son ampliamente usadas de manera independiente a nivel mundial en áreas, tales como: gobierno, negocios, industria, atención de salud y educación. Escasos artículos se han encontrado que relacionen ambas metodologías y ninguno que las utilice combinadas para determinar la eficiencia institucional en educación. Método: La contribución de este trabajo es presenter una metodología para medir la eficiencia de las escuelas de ingeniería de México entrenando una red neuronal artificial con la información generada por el AED. Para la evaluación de la eficiencia de de las 51 escuelas de ingeniería se utilizaron los indicadores del Programa Integral de Fortalecimiento Institucional (PIFI 2008- 2009. Resultados: Se obtuvieron resultados bastante satisfactorios, podría decirse que el modelo funciona a la perfección, esto es, introduciendo los valores del PIFI, se puede determinar el nivel de eficiencia educativo utilizando la red neuronal entrenada Conclusión: El impacto inmediato del trabajo propuesto es que no solamente se puede determinar la eficiencia institucional de DES, sino que se puede aplicar la metodología a otras instituciones, tanto públicas como privadas.

  17. Étude des impacts écologiques du dynamisme spatio-temporel des ...

    African Journals Online (AJOL)

    4 nov. 2013 ... du changement des habitats naturels sur les espèces menacées de sa faune. ... le contexte des changements climatiques. La situation de la .... naturels des espèces de la faune menacée dans la région évo- luent au cours du ... distribution des espèces cibles a ensuite été cartographiée sur la couche ...

  18. Beeinflussung des Essentiellen Tremors und des Tremors beim Morbus Parkinson durch transkranielle Gleichstromstimulation

    OpenAIRE

    Hellriegel, Helge

    2010-01-01

    Ziel dieser Arbeit war es die Rolle des primärmotorischen Kortex bei der Entstehung und Weiterleitung des Essentiellen Tremors und des Tremors beim Morbus Parkinson zu untersuchen. Hierzu wurde eine transkranielle Gleichstromstimulation über dem primärmotorischen Kortex bei Patienten mit oben genannten Tremorsyndromen durchgeführt und die klinischen sowie elektrophysiologisch messbaren Änderungen des Tremors beurteilt.

  19. Nuclear progesterone receptor isoforms and their functions in the female reproductive tract.

    Science.gov (United States)

    Rekawiecki, R; Kowalik, M K; Kotwica, J

    2011-01-01

    Progesterone (P4), which is produced by the corpus luteum (CL), creates proper conditions for the embryo implantation, its development, and ensures proper conditions for the duration of pregnancy. Besides the non-genomic activity of P4 on target cells, its main physiological effect is caused through genomic action by the progesterone nuclear receptor (PGR). This nuclear progesterone receptor occurs in two specific isoforms, PGRA and PGRB. PGRA isoform acts as an inhibitor of transcriptional action of PGRB. The inactive receptor is connected with chaperone proteins and attachment of P4 causes disconnection of chaperones and unveiling of DNA binding domain (DBD). After receptor dimerization in the cells' nucleus and interaction with hormone response element (HRE), the receptor coactivators are connected and transcription is initiated. The ratio of these isoforms changes during the estrous cycle and reflects the different levels of P4 effect on the reproductive system. Both isoforms, PGRA and PGRB, also show a different response to the P4 receptor antagonist activity. Connection of the antagonist to PGRA can block PGRB, but acting through the PGRB isoform, P4 receptor antagonist may undergo conversion to a strongly receptor agonist. A third isoform, PGRC, has also been revealed. This isoform is the shortest and does not have transcriptional activity. Alternative splicing and insertion of additional exons may lead to the formation of different PGR isoforms. This paper summarizes the available data on the progesterone receptor isoforms and its regulatory action within the female reproductive system.

  20. Identification of signals that facilitate isoform specific nucleolar localization of myosin IC.

    Science.gov (United States)

    Schwab, Ryan S; Ihnatovych, Ivanna; Yunus, Sharifah Z S A; Domaradzki, Tera; Hofmann, Wilma A

    2013-05-01

    Myosin IC is a single headed member of the myosin superfamily that localizes to the cytoplasm and the nucleus, where it is involved in transcription by RNA polymerases I and II, intranuclear transport, and nuclear export. In mammalian cells, three isoforms of myosin IC are expressed that differ only in the addition of short isoform-specific N-terminal peptides. Despite the high sequence homology, the isoforms show differences in cellular distribution, in localization to nuclear substructures, and in their interaction with nuclear proteins through yet unknown mechanisms. In this study, we used EGFP-fusion constructs that express truncated or mutated versions of myosin IC isoforms to detect regions that are involved in isoform-specific localization. We identified two nucleolar localization signals (NoLS). One NoLS is located in the myosin IC isoform B specific N-terminal peptide, the second NoLS is located upstream of the neck region within the head domain. We demonstrate that both NoLS are functional and necessary for nucleolar localization of specifically myosin IC isoform B. Our data provide a first mechanistic explanation for the observed functional differences between the myosin IC isoforms and are an important step toward our understanding of the underlying mechanisms that regulate the various and distinct functions of myosin IC isoforms.

  1. PRONÓSTICO DE VENTAS: COMPARACIÓN DE PREDICCIÓN ENTRE REDES NEURONALES Y MÉTODO ESTADÍSTICO

    Directory of Open Access Journals (Sweden)

    S. Nojek

    2003-05-01

    -family:"Calibri","sans-serif"; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-fareast-font-family:"Times New Roman"; mso-fareast-theme-font:minor-fareast; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin; mso-bidi-font-family:"Times New Roman"; mso-bidi-theme-font:minor-bidi;}

    RESUMEN

    Actualmente es común que las empresas realicen pronósticos de ventas para poder planificar más acertadamente su producción. El objetivo de este trabajo consiste en estudiar la predicción de ventas mediante el uso de redes neuronales, comparar los resultados obtenidos con los pronósticos de un método estadístico clásico y establecer los entornos más adecuados para su uso.

    Palabras Clave: Pronostico de Ventas – Predicción basada en Redes Neuronales.

     

    ABSTRACT

    Companies usually need sales forecast for improving production planning. This research work pursues to study sales forecast based on neural networks and to compare this results with the ones based on classical statistical methods trying to specify the adequate business environment for using them

    Keywords: Sales Forecast – Forecasting based on Neural Networks

  2. Contribution à la Gestion des Cercosporioses du bananier dues à ...

    African Journals Online (AJOL)

    SARAH

    31 mai 2017 ... the state of disease progression after each treatment. Results and applications ..... 2007, des logiciels SPSS (Statistical Package for Social. Sciences) version ... un facteur suivies d'un test de comparaison des moyennes des ...

  3. La gestion des adoptions internationales

    OpenAIRE

    Boéchat, Hervé

    2011-01-01

    Après les rumeurs nées lors du tsunami et le désastre avéré de L’Arche de Zoé, l’adoption – et plus généralement le sort des enfants – est devenue une préoccupation majeure en cas de catastrophe naturelle ou de conflit. Haïti, pays depuis longtemps ouvert à l’adoption internationale, n’a pas échappé à la polémique. Hervé Boéchat nous livre l’appréciation de son institution sur cet épisode qui n’a sans doute pas contribué à apaiser le ressentiment des Haïtiens contre la « communauté internati...

  4. GABAB(1) receptor subunit isoforms differentially regulate stress resilience

    Science.gov (United States)

    O’Leary, Olivia F.; Felice, Daniela; Galimberti, Stefano; Savignac, Hélène M.; Bravo, Javier A.; Crowley, Tadhg; El Yacoubi, Malika; Vaugeois, Jean-Marie; Gassmann, Martin; Bettler, Bernhard; Dinan, Timothy G.; Cryan, John F.

    2014-01-01

    Stressful life events increase the susceptibility to developing psychiatric disorders such as depression; however, many individuals are resilient to such negative effects of stress. Determining the neurobiology underlying this resilience is instrumental to the development of novel and more effective treatments for stress-related psychiatric disorders. GABAB receptors are emerging therapeutic targets for the treatment of stress-related disorders such as depression. These receptors are predominantly expressed as heterodimers of a GABAB(2) subunit with either a GABAB(1a) or a GABAB(1b) subunit. Here we show that mice lacking the GABAB(1b) receptor isoform are more resilient to both early-life stress and chronic psychosocial stress in adulthood, whereas mice lacking GABAB(1a) receptors are more susceptible to stress-induced anhedonia and social avoidance compared with wild-type mice. In addition, increased hippocampal expression of the GABAB(1b) receptor subunit is associated with a depression-like phenotype in the helpless H/Rouen genetic mouse model of depression. Stress resilience in GABAB(1b)−/− mice is coupled with increased proliferation and survival of newly born cells in the adult ventral hippocampus and increased stress-induced c-Fos activation in the hippocampus following early-life stress. Taken together, the data suggest that GABAB(1) receptor subunit isoforms differentially regulate the deleterious effects of stress and, thus, may be important therapeutic targets for the treatment of depression. PMID:25288769

  5. Entropy-based model for miRNA isoform analysis.

    Directory of Open Access Journals (Sweden)

    Shengqin Wang

    Full Text Available MiRNAs have been widely studied due to their important post-transcriptional regulatory roles in gene expression. Many reports have demonstrated the evidence of miRNA isoform products (isomiRs in high-throughput small RNA sequencing data. However, the biological function involved in these molecules is still not well investigated. Here, we developed a Shannon entropy-based model to estimate isomiR expression profiles of high-throughput small RNA sequencing data extracted from miRBase webserver. By using the Kolmogorov-Smirnov statistical test (KS test, we demonstrated that the 5p and 3p miRNAs present more variants than the single arm miRNAs. We also found that the isomiR variant, except the 3' isomiR variant, is strongly correlated with Minimum Free Energy (MFE of pre-miRNA, suggesting the intrinsic feature of pre-miRNA should be one of the important factors for the miRNA regulation. The functional enrichment analysis showed that the miRNAs with high variation, particularly the 5' end variation, are enriched in a set of critical functions, supporting these molecules should not be randomly produced. Our results provide a probabilistic framework for miRNA isoforms analysis, and give functional insights into pre-miRNA processing.

  6. Expression of aquaporin isoforms during human and mouse tooth development.

    Science.gov (United States)

    Felszeghy, S; Módis, L; Németh, P; Nagy, G; Zelles, T; Agre, P; Laurikkala, J; Fejerskov, O; Thesleff, I; Nielsen, S

    2004-04-01

    Previously, we described the development of hyaluronan (HA) deposition in human tooth germ tissues that are consistent with water transport in different stages of tooth development. The aquaporins (AQP) constitute a family of membrane water channels that are expressed in many organs. However, there are no data available about the expression pattern of aquaporin water channels in dental structures. In the present study we have characterised the expression of six different aquaporin isoforms (AQP1-5, AQP-9) in developing human and mouse tooth germs by immunohistochemistry using isoform specific antibodies. In the "bell stage" AQP1 was expressed in endothelial cells of small vessels whereas no other structures of the tooth primordial were labeled. AQP2, AQP3 and AQP9 immunoreactivity was not observed in tooth germs, whereas strong AQP4 and AQP5 expression was observed in dental lamina, inner enamel epithelium, stratum intermedium, stellate reticulum and the outer enamel epithelium. Oral epithelium also exhibited AQP4 and AQP5 immunolabeling. During development of the matrices of the dental hard tissues AQP4 and AQP5 immunostaining was observed in the odontoblasts and their processes, as well as in the secretory ameloblast and their apical processes. Immunolabeling controls were negative. In conclusion, AQP4 and AQP5 are expressed in tooth germ tissues in early development in cells that previously have been shown to express HA and/or CD44, indicating that AQP water channels may play a role for ECM hydration during tooth development.

  7. A Review of Metallothionein Isoforms and their Role in Pathophysiology

    Directory of Open Access Journals (Sweden)

    Senthil kumar M

    2011-05-01

    Full Text Available Abstract The Metallothionein (MT is a protein which has several interesting biological effects and has been demonstrated increase focus on the role of MT in various biological systems in the past three decades. The studies on the role of MT were limited with few areas like apoptosis and antioxidants in selected organs even fifty years after its discovery. Now acknowledge the exploration of various isoforms of MT such as MT-I, MT-II, MT-III and MT-IV and other isoforms in various biological systems. Strong evidence exists that MT modulates complex diseases and the immune system in the body but the primary function of MT still remains unknown. This review's main objective is to explore the capability to specifically manipulate MT levels in cells and in animals to provide answers regarding how MT could impact those complex disease scenarios. The experimental result mentioned in this review related among MT, zinc, cadmium, diabetic, heart disease, bone retardation, neuro toxicity, kidney dysfunction, cancer, and brain suggest novel method for exploration and contribute significantly to the growing scientist to research further in this field.

  8. Structures of alternatively spliced isoforms of human ketohexokinase.

    Science.gov (United States)

    Trinh, Chi H; Asipu, Aruna; Bonthron, David T; Phillips, Simon E V

    2009-03-01

    A molecular understanding of the unique aspects of dietary fructose metabolism may be the key to understanding and controlling the current epidemic of fructose-related obesity, diabetes and related adverse metabolic states in Western populations. Fructose catabolism is initiated by its phosphorylation to fructose 1-phosphate, which is performed by ketohexokinase (KHK). Here, the crystal structures of the two alternatively spliced isoforms of human ketohexokinase, hepatic KHK-C and the peripheral isoform KHK-A, and of the ternary complex of KHK-A with the substrate fructose and AMP-PNP are reported. The structure of the KHK-A ternary complex revealed an active site with both the substrate fructose and the ATP analogue in positions ready for phosphorylation following a reaction mechanism similar to that of the pfkB family of carbohydrate kinases. Hepatic KHK deficiency causes the benign disorder essential fructosuria. The effects of the disease-causing mutations (Gly40Arg and Ala43Thr) have been modelled in the context of the KHK structure.

  9. BDNF isoforms: a round trip ticket between neurogenesis and serotonin?

    Science.gov (United States)

    Foltran, Rocío Beatriz; Diaz, Silvina Laura

    2016-07-01

    The brain-derived neurotrophic factor, BDNF, was discovered more than 30 years ago and, like other members of the neurotrophin family, this neuropeptide is synthetized as a proneurotrophin, the pro-BDNF, which is further cleaved to yield mature BDNF. The myriad of actions of these two BDNF isoforms in the central nervous system is constantly increasing and requires the development of sophisticated tools and animal models to refine our understanding. This review is focused on BDNF isoforms, their participation in the process of neurogenesis taking place in the hippocampus of adult mammals, and the modulation of their expression by serotonergic agents. Interestingly, around this triumvirate of BDNF, serotonin, and neurogenesis, a series of recent research has emerged with apparently counterintuitive results. This calls for an exhaustive analysis of the data published so far and encourages thorough work in the quest for new hypotheses in the field. BDNF is synthetized as a pre-proneurotrophin. After removal of the pre-region, proBDNF can be cleaved by intracellular or extracellular proteases. Mature BDNF can bind TrkB receptors, promoting their homodimerization and intracellular phosphorylation. Phosphorylated-TrkB can activate three different signaling pathways. Whereas G-protein-coupled receptors can transactivate TrkB receptors, truncated forms can inhibit mBDNF signaling. Pro-BDNF binds p75(NTR) by its mature domain, whereas the pro-region binds co-receptors. © 2016 International Society for Neurochemistry.

  10. Catalogue ostéologique des oiseaux

    NARCIS (Netherlands)

    Oort, van E.D.

    1907-01-01

    Je le crois utile de faire précéder le présent Catalogue par quelques remarques. J’ai énuméré autant que possible les parties, qui font défaut aux squelettes. L’absence de plusieurs osselets, comme des doigts, des vertèbres caudales, des côtes etc. au même échantillon, est indiqué par „incomplet” ou

  11. Le piratage des biens d'information

    OpenAIRE

    Paul Belleflamme

    2003-01-01

    Où se situe le juste milieu entre les propos alarmistes des uns (“le piratage tue la production musicale!”) et les appels libertaires des autres (“l'accès à toute information doit être libre!”) ? Une analyse économique simple nous permet de mieux comprendre les enjeux et de dégager des pistes de réflexion pour l'avenir.

  12. 79 Influences des rejets des eaux usées domestiques dans les ...

    African Journals Online (AJOL)

    PR BOKO

    L'usage agricole d'effluents liquides peut être considéré comme une ... Rares sont celles qui sont alimentées par une eau de source [8]. ... nutriments, des Eléments Traces Métalliques (ETM) [9], des micro- ... essentiels pour la croissance des plantes ; ils provoquent cependant une prolifération excessive d' algues. La.

  13. Présentation des textes

    OpenAIRE

    Freitag, Michel

    2015-01-01

    Les textes choisis n’ont pas pour but la reconstitution ou le survol d’une carrière, mais la mise en valeur des étapes saillantes d’une double éclosion, celle d’Elizabeth Cady Stanton comme féministe et avec elle celle du mouvement de défense des droits des femmes aux États-Unis. Un tel objectif implique donc des limites temporelles en amont et en aval de l’événement fondateur que fut la Convention de Seneca Falls en 1848, origine du texte non moins fondateur de la Déclaration de sentiments r...

  14. Annuaire du Bureau des longitudes - 2006

    Science.gov (United States)

    Imcce; Bureau Des Longitudes

    2005-07-01

    This annual publication provides ephemerides and data to the use of professionnal and amateur astronomers. Divided in 11 chapters it covers concordance of various calendars, explanation of fondamental astronomy and various time scales, explanation for the use of ephemerides; tables provide ephemerides (positions, rise/set/passage) of the Sun and the Moon, planets, planetary satellites, asteroids, comets, bright stars; data and explanation for the physical observation of the surface of the Sun, the Moon, and planets; chart of the sky and a list of constellations and galaxies; prediction and ephemerides for astronomical phenomenon: occultation by the moon, stellar occultations by asteroids and appulses, solar eclipses and lunar eclipses; and an additional review about a hot scientific topic, this year: "Legendre et le méridien terrestre, 200 ans après". Cette publication annuelle fournit des éphémérides et des données à l'usage des astronomes professionnels et des astronomes amateurs. Composée de 11 chapitres elle comprend les rubriques sur les différents calendriers et leurs concordance, les fêtes légales en France, les dates et décrets sur les heures légales en France métropolitaine ; une introduction à l'astronomie fondamentale et aux différentes échelles de temps, des explications sur l'utilisation des éphémérides ; des tables fournissent les éphémérides (positions, heures de lever/coucher/passage) du Soleil et de la Lune, de planètes, de satellites naturels, d'astéroïdes, de comètes, d'étoiles brillantes ; des données pour l'observation de la surface du Soleil, de la Lune, et des planètes ; des cartes du ciel ainsi qu'une liste de constellations et de galaxies ; des prédictions des phénomènes astronomiques : occultation par la Lune, occultation stellaires par des astéroïdes et appulses, éclipses de Soleil et de la Lune; la liste et les coordonnées des observatoires astronomiques les plus connus ; et enfin un cahier th

  15. Vivre et survivre au bord des villes

    Directory of Open Access Journals (Sweden)

    Michel Péraldi

    1996-04-01

    Full Text Available De quels revenus vivent, ou plutôt survivent, les populations des quartiers dits défavorisés ? Telle est, malgré son apparente banalité, la question qui initie aujourd'hui une partie de nos recherches. Question banale en effet puisque les médias et le débat politique nous donnent une réponse sans appel, statistiquement fiable : dans ces univers sociaux où se croisent des communautés issues des plus récentes migrations et les fractions les plus fragiles économiquement des classes populaires fr...

  16. La compaction des sols agricoles en Wallonie

    OpenAIRE

    Destain, Marie-France

    2014-01-01

    Dans nos pays industrialisés, l’agriculture fait appel à des machines de plus en plus lourdes. Lorsque des charges élevées sont appliquées sur des sols sensibles, une compaction du sol peut se produire conduisant à des diminutions de rendements suite aux difficultés que rencontrent les racines pour prélever l’eau et les nutriments et à la perturbation de l’activité de la pédofaune. Au niveau environnemental, du fait de la compaction, l’infiltration de l’eau dans le sol est réduite, princip...

  17. EFFET DES TRAITEMENTS THERMIQUES SUR LA REACTION ENTRE DES COUCHES MINCES DE TITANE ET DES SUBSTRATS EN ACIER

    Directory of Open Access Journals (Sweden)

    D Slimani

    2015-06-01

    Full Text Available Des couches minces du titane pur ont été déposées avec la méthode de pulvérisation cathodique sur des substrats en acier, type FF80 K-1 contenants ~1% mass. en carbone. La réaction entre les deux parties du système substrat-couche mince est activée avec des traitements thermiques sous vide dans l’intervalle de températures de 400 à900°Cpendant 30 minutes. Les Spectres de diffraction de rayons x confirment l’inter- diffusion des éléments  chimiques du système résultants la formation et la croissance des nouvelles phases en particulier le carbure binaire TiC ayant des caractéristiques thermomécaniques importantes. L’analyse morphologique des échantillons traités  avec le microscope électronique à balayage (MEB montre l’augmentation du flux de diffusion atomique avec la température de recuit, notamment la diffusion du manganèse et du fer vers la surface libre des échantillons aux températures élevées provoquant la dégradation des propriétés mécaniques des revêtements contrairement au premiers stades d’interaction où on a obtenu des bonnes valeurs de la microdureté.

  18. PREDICCIÓN DEL RENDIMIENTO DE UN CULTIVO DE NARANJA 'VALENCIA' MEDIANTE REDES NEURONALES DE REGRESIÓN GENERALIZADA // FORECASTING THE YIELD OF A 'VALENCIA' ORANGE´S CROP, BY MEANS OF A GENERALIZED-REGRESSION NEURAL NETWORKS

    Directory of Open Access Journals (Sweden)

    Edwin Hernández-Caraballo

    2015-12-01

    Full Text Available El rendimiento de un cultivo es el resultado de una multiplicidad de variables, cuyas complejas interacciones hacen que sea difícil de predecir por medios convencionales. Las redes neuronales de regresión generalizada constituyen una alternativa prometedora para dicha tarea, gracias a su capacidad para modelar relaciones no lineales, sin la necesidad de conocer la naturaleza explícita de las mismas. El presente trabajo tuvo como propósito evaluar dicha aproximación, en la predicción del rendimiento de un cultivo de naranja 'Valencia' (Citrus sinensis L. Osbeck, a partir de las concentraciones en el tejido foliar de nitrógeno, fósforo, potasio, calcio, y magnesio. Especial énfasis se hizo en el tratamiento de los datos de entrada/salida, utilizando técnicas convencionales (normalización, estandarización, y componentes principales, y otras no convencionales (cálculos de los log-cociente centrado, e índices nutricionales individuales nutricionales individuales y globales, a partir del Sistema de Diagnóstico de la Composición Nutricional. Los resultados mostraron que, entre las estudiadas, la combinación índices nutricionales individuales/rendimiento normalizado (Error de predicción= 0,98 kg·(1/árbol, y componentes principales no rotados/rendimiento normalizado (Error de predicción= 0,51 kg·(1/árbol condujeron al desarrollo de las redes neuronales con las mejores capacidades de predicción del rendimiento, evidenciadas por los menores errores de predicción anteriormente indicados. // ABSTRACT: The yield of a given crop is the result of a multiplicity of variables whose complex interactions make its prediction difficult to achieve by regular means. Generalized regression artificial neural networks represent a promising alternative for such a task, due to its ability to model non-linear relationships, without the need of knowing its explicit nature. The present work aimed at assessing such approximation for predicting the

  19. Aplicación de análisis de conglomerados y redes neuronales artificiales para la clasificación y selección de candidatos a residencias médicas

    Directory of Open Access Journals (Sweden)

    Raúl A. Borracci

    Full Text Available Introducción: Los programas educacionales han recurrido a distintos modelos de regresión lineal múltiple, de selección asistida por computadora y más recientemente, de redes neuronales artificiales para la confección de listados preliminares de mérito entre los postulantes a la residencia. Objetivo: evaluar y rediseñar un sistema para la selección y clasificación de aspirantes a un programa de residencias universitarias por medio de la aplicación de modelos de análisis multivariante y de redes neuronales artificiales. Material y Método: El diseño consistió en un estudio retrospectivo-transversal, realizado en un hospital universitario. Se evaluó una muestra al azar de 213 aspirantes a un programa de residencias médicas universitarias teniendo en cuenta el promedio de la carrera de grado, el resultado del examen de ingreso a la residencia, los antecedentes curriculares y biográficos, el internado y el puntaje de las entrevistas. Se aplicó un análisis de conglomerados jerárquico (clúster análisis para la clasificación y selección de los candidatos en un orden de mérito en base a los puntajes estandarizados de las 5 variables. Resultados: El análisis de conglomerados jerárquico clasificó 209 aspirantes en 12 conglomerados en base al promedio estandarizado de los valores obtenidos de las 5 variables. Este análisis se usó para construir una clasificación descriptiva de los grupos y una lista final por mérito de acuerdo a la posición relativa de cada candidato por encima o debajo de los puntajes promedios. Se imitó la solución de conglomerados por medio de una red perceptrón multicapa con una sensibilidad y especificidad de 94.1 y 99.1% respectivamente. Conclusiones: El análisis de conglomerados jerárquico fue un método útil y novedoso para clasificar una muestra de aspirantes a la residencia en conglomerados de acuerdo a la posición relativa de sus puntajes estandarizados por encima o por debajo de la media

  20. PREDICCIÓN DEL RENDIMIENTO DE UN CULTIVO DE PLÁTANO MEDIANTE REDES NEURONALES ARTIFICIALES DE REGRESIÓN GENERALIZADA // PREDICTION OF THE YIELD OF BANANA CROP BY MEANS OF A GENERALIZED REGRESSION ARTIFICIAL NEURAL NETWORK

    Directory of Open Access Journals (Sweden)

    Rita Ávila de Hernández

    2012-06-01

    Full Text Available Banana is an important crop in Latin America, for both large scale and small farmers. In Venezuela, the main production centers are in the area of Sur del Lago de Maracaibo. Knowing the crop yield is vital due to the need to maximize the investment-pro_t, and availability of such information in advance helps in the decision-making process of the production unit. The purpose of this research is to evaluate the ability of artificial neural networks to predict the yield of a banana crop, employing the best predictors dataset, given the physical characteristics of the soil and the chemical profile of leaf tissue. Generalized regression networks trained with the leave-one-out strategy, and two types of data transformations were used in this study. It was found that neural networks were excellent tools for predicting the yield of banana crop. The physicochemical profiles of soil and leaf tissue were suitable descriptors of the response variable. Among the data evaluated in this study, it was found that the physical data of 20-40 cm of soil was, after standardization of the training data, the best predictor group. // RESUMEN: El plátano es un cultivo importante en Latinoamérica, tanto a gran escala como para pequeños hacendados. En Venezuela, los principales centros productivos están en la zona del Sur del Lago de Maracaibo. Conocer el rendimiento de un cultivo obedece a la necesidad de maximizar la relación inversión-ganancia y la disponibilidad de esa información, con anticipación, permite tomar decisiones sobre el manejo de una plantación. La finalidad de esta investigación es evaluar la capacidad de las redes neuronales artificiales para predecir el rendimiento de un cultivo de plátano, empleando para ello el mejor grupo de datos predictores, determinado entre las características físicas del suelo y el perfil químico del tejido foliar. Se emplean redes de regresión generalizada y la estrategia leave-one-out, así como dos tipos de

  1. Differential sensitivity of rat voltage-sensitive sodium channel isoforms to pyrazoline-type insecticides.

    Science.gov (United States)

    Silver, Kristopher S; Soderlund, David M

    2006-07-15

    Pyrazoline-type insecticides are potent inhibitors of insect and mammalian voltage-sensitive sodium channels. In mammals, there are nine sodium channel alpha subunit isoforms that have unique distributions and pharmacological properties, but no published data exist that compare the relative sensitivity of these different mammalian sodium channel isoforms to inhibition by pyrazoline-type insecticides. This study employed the Xenopus oocyte expression system to examine the relative sensitivity of rat Na(v)1.2a, Na(v)1.4, Na(v)1.5, and Na(v)1.8 sodium channel alpha subunit isoforms to the pyrazoline-type insecticides indoxacarb, DCJW, and RH 3421. Additionally, we assessed the effect of coexpression with the rat beta1 auxiliary subunit on the sensitivity of the Na(v)1.2a and Na(v)1.4 isoforms to these compounds. The relative sensitivity of the four sodium channel alpha subunits differed for each of the three compounds we examined. With DCJW, the order of sensitivity was Na(v)1.4 > Na(v)1.2a > Na(v)1.5 > Na(v)1.8. In contrast, the relative sensitivity of these isoforms to indoxacarb differed from that to DCJW: the Na(v)1.8 isoform was most sensitive, the Na(v)1.4 isoform was completely insensitive, and the sensitivities of the Na(v)1.5 and Na(v)1.2a isoforms were intermediate between these two extremes. Moreover, the pattern of sensitivity to RH 3421 among these four isoforms was different from that for either indoxacarb or DCJW: the Na(v)1.4 isoform was most sensitive to RH 3421, whereas the sensitivities of the remaining three isoforms were substantially less than that of the Na(v)1.4 isoform and were approximately equivalent. The only statistically significant effect of coexpression of either the Na(v)1.2a or Na(v)1.4 isoforms with the beta1 subunit was the modest reduction in the sensitivity of the Na(v)1.2a isoform to RH 3421. These results demonstrate that mammalian sodium channel isoforms differ in their sensitivities to pyrazoline-type insecticides.

  2. Predicción del color y contenido de humedad en café cerezo mediante redes neuronales y regresión de mínimos cuadrados parciales

    Directory of Open Access Journals (Sweden)

    Wilson Manuel Castro Silupu

    2015-12-01

    Full Text Available La presente investigación se enfocó en el desarrollo de modelos de predicción del color en coordenadas CIELab y el contenido de humedad de café cerezo mediante la tecnología de imágenes hiperespectrales; comparando el ajuste por un modelo de regresión lineal múltiple – PLSR (Partialleastsquareregression y un modelo no lineal (ANN – artiftial neural network. La muestra se conformó de 200 granos de café cerezo en diferentes estados de madurez, dividiéndola en 120 granos para calibración y 80 de validación.La  muestra fue caracterizada mediante colorimetría en el espacio CIELab y determinación de la humedad. Posteriormente se adquirieron imágenes hiperespectrales de cada granos y se almacenaron en formato *.bil. El procesamiento de las imágenes se realizó mediante un sistema desarrollado e implementado en el software matemático Matlab 2010a, mediante funciones *.m e interfaces de usuario (GUIs. Se desarrollaron modelos de ajuste para cada una de las coordenadas de color y el contenido de humedad, calculándose los coeficientes de correlación en calibración y validación. Los resultados mostraron que las redes neuronales tienen un mayor ajuste en calibración con coeficientes de correlación superiores a 0,90 mientras que el PLSR genero coeficientes entre 0,42 y 0,48.

  3. Modelamiento matemático y por redes neuronales artificiales del crecimiento de Spirulina sp. en fotobiorreactor con fuente de luz fluorescente e iluminación en estado sólido

    Directory of Open Access Journals (Sweden)

    Víctor Vásquez-Villalobos

    2013-01-01

    Full Text Available Se evaluaron los modelos matemáticos de Gompertz y logístico en la cinética de crecimiento de Spirulina sp., los cuales fueron comparados con un modelamiento por Redes Neuronales Artificiales Backpropagation (RNA-BP. La Spirulina fue cultivada en un fotobiorreactor de laboratorio aireado (3 L/min de 500 mL, con iluminación fluorescente de 40W y en Estado Sólido (LED-Light Emitting Diode de 1W; obteniendo con ambos sistemas 11,0 klx. La iluminación LED, permitió obtener un valor elevado de biomasa (ɑ de 0,90 , en comparación con la obtenida con iluminación fluorescente de 0,82; así como una mayor velocidad de crecimiento μ=0,63 h-1, precedida de un menor tiempo de latencia λ=0,34 h. La RNA-BP mostró buena precisión con respecto al modelo corregido de Gompertz I, tanto para el caso del cultivo de Spirulina sp. con iluminación fluorescente y con LED, mostrando coeficientes de correlación (R del orden de 0,993 y 0,994 respectivamente, con respecto a los datos experimentales. Resulta ventajoso el modelamiento a través del modelo corregido de Gompertz I, porque además de valores de R de 0,987 y 0,990 en los cultivos de Spirulina sp. Con iluminación fluorescente y con LED respectivamente, permite obtener los parámetros de la cinética de crecimiento de manera directa.

  4. Predicción por redes neuronales artificiales de la calidad fisicoquímica de vinagre de melaza de caña por efecto de tiempo-temperatura de alimentación a evaporador-destilador flash

    Directory of Open Access Journals (Sweden)

    Víctor Vásquez V.

    2010-01-01

    Full Text Available Se predijo por Redes Neuronales Artificiales (RNA importantes características fisicoquímicas de vinagre de melaza: pH, densidad, acidez total, etanol, aldehídos totales y furfural; obtenidas mediante operaciones de evaporación flash y clarificación por destilación flash. Melaza fermentada por vía alcohólica y acética, fue alimentada a un evaporador flash a cuatro temperaturas (61, 66, 71 y 76 °C y tres tiempos (25, 35 y 45 min. La predicción se realizó con dos redes: RNA-A y RNA-B, ambas con buen desempeño. La RNA-A fue del tipo feedforward (FF, con algoritmos de entrenamiento Backpropagation (BP y ajuste de pesos Levenberg-Marquardt (LM, topología: 6 entradas (datos de las operaciones de evaporación-destilación flash, 7 salidas lineales (características fisicoquímicas, 9 neuronas tangente sigmoidales en 1 capa oculta, coeficiente de momento 0.5, tasa de aprendizaje 0.01, meta del error 0.0001 y 20 etapas de entrenamiento. La RNA-A mostró mejor desempeño que un modelo estadístico de primer orden. La RNA-B igualmente FF, con algoritmos BP y LM, topología: 2 entradas (datos de la evaporación flash, 7 salidas lineales (características fisicoquímicas, 84 neuronas logaritmo sigmoidales en 1 capa oculta, coeficiente de momento 0.5, tasa de aprendizaje 0.01, meta del error 0.0001 y 300 etapas de entrenamiento. La RNA-B mostró igual capacidad predictiva que un modelo estadístico de primer orden con interacción de términos.

  5. Nesprins: tissue-specific expression of epsilon and other short isoforms.

    Directory of Open Access Journals (Sweden)

    Nguyen Thuy Duong

    Full Text Available Nesprin-1-giant and nesprin-2-giant regulate nuclear positioning by the interaction of their C-terminal KASH domains with nuclear membrane SUN proteins and their N-terminal calponin-homology domains with cytoskeletal actin. A number of short isoforms lacking the actin-binding domains are produced by internal promotion. We have evaluated the significance of these shorter isoforms using quantitative RT-PCR and western blotting with site-specific monoclonal antibodies. Within a complete map of nesprin isoforms, we describe two novel nesprin-2 epsilon isoforms for the first time. Epsilon isoforms are similar in size and structure to nesprin-1-alpha. Expression of nesprin isoforms was highly tissue-dependent. Nesprin-2-epsilon-1 was found in early embryonic cells, while nesprin-2-epsilon-2 was present in heart and other adult tissues, but not skeletal muscle. Some cell lines lack shorter isoforms and express only one of the two nesprin genes, suggesting that either of the giant nesprins is sufficient for basic cell functions. For the first time, localisation of endogenous nesprin away from the nuclear membrane was shown in cells where removal of the KASH domain by alternative splicing occurs. By distinguishing between degradation products and true isoforms on western blots, it was found that previously-described beta and gamma isoforms are expressed either at only low levels or with a limited tissue distribution. Two of the shortest alpha isoforms, nesprin-1-alpha-2 and nesprin-2-alpha-1, were found almost exclusively in cardiac and skeletal muscle and a highly conserved and alternatively-spliced exon, available in both nesprin genes, was always included in these tissues. These "muscle-specific" isoforms are thought to form a complex with emerin and lamin A/C at the inner nuclear membrane and mutations in all three proteins cause Emery-Dreifuss muscular dystrophy and/or inherited dilated cardiomyopathy, disorders in which only skeletal muscle and

  6. Expression of phosphoinositide-specific phospholipase C isoforms in native endothelial cells.

    Directory of Open Access Journals (Sweden)

    Delphine M Béziau

    Full Text Available Phospholipase C (PLC comprises a superfamily of enzymes that play a key role in a wide array of intracellular signalling pathways, including protein kinase C and intracellular calcium. Thirteen different mammalian PLC isoforms have been identified and classified into 6 families (PLC-β, γ, δ, ε, ζ and η based on their biochemical properties. Although the expression of PLC isoforms is tissue-specific, concomitant expression of different PLC has been reported, suggesting that PLC family is involved in multiple cellular functions. Despite their critical role, the PLC isoforms expressed in native endothelial cells (ECs remains undetermined. A conventional PCR approach was initially used to elucidate the mRNA expression pattern of PLC isoforms in 3 distinct murine vascular beds: mesenteric (MA, pulmonary (PA and middle cerebral arteries (MCA. mRNA encoding for most PLC isoforms was detected in MA, MCA and PA with the exception of η2 and β2 (only expressed in PA, δ4 (only expressed in MCA, η1 (expressed in all but MA and ζ (not detected in any vascular beds tested. The endothelial-specific PLC expression was then sought in freshly isolated ECs. Interestingly, the PLC expression profile appears to differ across the investigated arterial beds. While mRNA for 8 of the 13 PLC isoforms was detected in ECs from MA, two additional PLC isoforms were detected in ECs from PA and MCA. Co-expression of multiple PLC isoforms in ECs suggests an elaborate network of signalling pathways: PLC isoforms may contribute to the complexity or diversity of signalling by their selective localization in cellular microdomains. However in situ immunofluorescence revealed a homogeneous distribution for all PLC isoforms probed (β3, γ2 and δ1 in intact endothelium. Although PLC isoforms play a crucial role in endothelial signal transduction, subcellular localization alone does not appear to be sufficient to determine the role of PLC in the signalling microdomains found

  7. Transport quantique dans des nanostructures

    Science.gov (United States)

    Naud, C.

    2002-09-01

    structure des oscillations de conductance en fonction du flux du champ magnétique de période h/e dont l'amplitude est beaucoup plus importante que celle mesurée sur un réseau carré de même dimension. Cette différence constitue une signature d'un effet de localisation induit par le champ magnétique sur la topologie mathcal{T}3. Pour des valeurs spécifiques du champ magnétique, du fait des interférences destructives Aharonov-Bohm, la propagation des fonctions d'ondes est limitée à un ensemble fini de cellule du réseau appelé cage. De la dépendance en température des oscillations de période h/e mesurées sur le réseau mathcal{T}3 nous avons tiré une longueur caractéristique qui peut être rattachée au périmètre des cages. Un phénomène inattendu fut l'observation, pour des champs magnétiques plus importants, d'un doublement de fréquence des oscillations. Ces oscillations de période h/2e pouvant avoir une amplitude supérieure aux oscillations de période h/e, une interprétation en terme d'harmonique n'est pas possible. Enfin, l'influence de la largeur électrique des fils constituant le réseau et donc celle du nombre de canaux par brin a été étudiée en réalisant des grilles électrostatique. Les variations de l'amplitude des signaux en h/e et h/2e en fonction de la tension de grille ont été mesurés.

  8. Impact de la preparation des anodes crues et des conditions de cuisson sur la fissuration dans des anodes denses

    Science.gov (United States)

    Amrani, Salah

    La fabrication de l'aluminium est realisee dans une cellule d'electrolyse, et cette operation utilise des anodes en carbone. L'evaluation de la qualite de ces anodes reste indispensable avant leur utilisation. La presence des fissures dans les anodes provoque une perturbation du procede l'electrolyse et une diminution de sa performance. Ce projet a ete entrepris pour determiner l'impact des differents parametres de procedes de fabrication des anodes sur la fissuration des anodes denses. Ces parametres incluent ceux de la fabrication des anodes crues, des proprietes des matieres premieres et de la cuisson. Une recherche bibliographique a ete effectuee sur tous les aspects de la fissuration des anodes en carbone pour compiler les travaux anterieurs. Une methodologie detaillee a ete mise au point pour faciliter le deroulement des travaux et atteindre les objectifs vises. La majorite de ce document est reservee pour la discussion des resultats obtenus au laboratoire de l'UQAC et au niveau industriel. Concernant les etudes realisees a l'UQAC, une partie des travaux experimentaux est reservee a la recherche des differents mecanismes de fissuration dans les anodes denses utilisees dans l'industrie d'aluminium. L'approche etait d'abord basee sur la caracterisation qualitative du mecanisme de la fissuration en surface et en profondeur. Puis, une caracterisation quantitative a ete realisee pour la determination de la distribution de la largeur de la fissure sur toute sa longueur, ainsi que le pourcentage de sa surface par rapport a la surface totale de l'echantillon. Cette etude a ete realisee par le biais de la technique d'analyse d'image utilisee pour caracteriser la fissuration d'un echantillon d'anode cuite. L'analyse surfacique et en profondeur de cet echantillon a permis de voir clairement la formation des fissures sur une grande partie de la surface analysee. L'autre partie des travaux est basee sur la caracterisation des defauts dans des echantillons d'anodes crues

  9. Different phosphoinositide 3-kinase isoforms mediate carrageenan nociception and inflammation.

    Science.gov (United States)

    Pritchard, Rory A; Falk, Lovissa; Larsson, Mathilda; Leinders, Mathias; Sorkin, Linda S

    2016-01-01

    Phosphoinositide 3-kinases (PI3Ks) participate in signal transduction cascades that can directly activate and sensitize nociceptors and enhance pain transmission. They also play essential roles in chemotaxis and immune cell infiltration leading to inflammation. We wished to determine which PI3K isoforms were involved in each of these processes. Lightly anesthetized rats (isoflurane) were injected subcutaneously with carrageenan in their hind paws. This was preceded by a local injection of 1% DMSO vehicle or an isoform-specific antagonist to PI3K-α (compound 15-e), -β (TGX221), -δ (Cal-101), or -γ (AS252424). We measured changes in the mechanical pain threshold and spinal c-Fos expression (4 hours after injection) as indices of nociception. Paw volume, plasma extravasation (Evans blue, 0.3 hours after injection), and neutrophil (myeloperoxidase; 1 hour after injection) and macrophage (CD11b+; 4 hour after injection) infiltration into paw tissue were the measured inflammation endpoints. Only PI3K-γ antagonist before treatment reduced the carrageenan-induced pain behavior and spinal expression of c-Fos (P ≤ 0.01). In contrast, pretreatment with PI3K-α, -δ, and-γ antagonists reduced early indices of inflammation. Plasma extravasation PI3K-α (P ≤ 0.05), -δ (P ≤ 0.05), and -γ (P ≤ 0.01), early (0-2 hour) edema -α (P ≤ 0.05), -δ (P ≤ 0.001), and -γ (P ≤ 0.05), and neutrophil infiltration (all P ≤ 0.001) were all reduced compared to vehicle pretreatment. Later (2-4 hour), edema and macrophage infiltration (P ≤ 0.05) were reduced by only the PI3K-δ and -γ isoform antagonists, with the PI3K-δ antagonist having a greater effect on edema. PI3K-β antagonism was ineffective in all paradigms. These data indicate that pain and clinical inflammation are pharmacologically separable and may help to explain clinical conditions in which inflammation naturally wanes or goes into remission, but pain continues unabated.

  10. Using XML to encode TMA DES metadata

    Directory of Open Access Journals (Sweden)

    Oliver Lyttleton

    2011-01-01

    Full Text Available Background: The Tissue Microarray Data Exchange Specification (TMA DES is an XML specification for encoding TMA experiment data. While TMA DES data is encoded in XML, the files that describe its syntax, structure, and semantics are not. The DTD format is used to describe the syntax and structure of TMA DES, and the ISO 11179 format is used to define the semantics of TMA DES. However, XML Schema can be used in place of DTDs, and another XML encoded format, RDF, can be used in place of ISO 11179. Encoding all TMA DES data and metadata in XML would simplify the development and usage of programs which validate and parse TMA DES data. XML Schema has advantages over DTDs such as support for data types, and a more powerful means of specifying constraints on data values. An advantage of RDF encoded in XML over ISO 11179 is that XML defines rules for encoding data, whereas ISO 11179 does not. Materials and Methods: We created an XML Schema version of the TMA DES DTD. We wrote a program that converted ISO 11179 definitions to RDF encoded in XML, and used it to convert the TMA DES ISO 11179 definitions to RDF. Results: We validated a sample TMA DES XML file that was supplied with the publication that originally specified TMA DES using our XML Schema. We successfully validated the RDF produced by our ISO 11179 converter with the W3C RDF validation service. Conclusions: All TMA DES data could be encoded using XML, which simplifies its processing. XML Schema allows datatypes and valid value ranges to be specified for CDEs, which enables a wider range of error checking to be performed using XML Schemas than could be performed using DTDs.

  11. Using XML to encode TMA DES metadata.

    Science.gov (United States)

    Lyttleton, Oliver; Wright, Alexander; Treanor, Darren; Lewis, Paul

    2011-01-01

    The Tissue Microarray Data Exchange Specification (TMA DES) is an XML specification for encoding TMA experiment data. While TMA DES data is encoded in XML, the files that describe its syntax, structure, and semantics are not. The DTD format is used to describe the syntax and structure of TMA DES, and the ISO 11179 format is used to define the semantics of TMA DES. However, XML Schema can be used in place of DTDs, and another XML encoded format, RDF, can be used in place of ISO 11179. Encoding all TMA DES data and metadata in XML would simplify the development and usage of programs which validate and parse TMA DES data. XML Schema has advantages over DTDs such as support for data types, and a more powerful means of specifying constraints on data values. An advantage of RDF encoded in XML over ISO 11179 is that XML defines rules for encoding data, whereas ISO 11179 does not. We created an XML Schema version of the TMA DES DTD. We wrote a program that converted ISO 11179 definitions to RDF encoded in XML, and used it to convert the TMA DES ISO 11179 definitions to RDF. We validated a sample TMA DES XML file that was supplied with the publication that originally specified TMA DES using our XML Schema. We successfully validated the RDF produced by our ISO 11179 converter with the W3C RDF validation service. All TMA DES data could be encoded using XML, which simplifies its processing. XML Schema allows datatypes and valid value ranges to be specified for CDEs, which enables a wider range of error checking to be performed using XML Schemas than could be performed using DTDs.

  12. APPRENTISSAGE DES LANGUES AUTOUR DES MéDIATIONS ARTISTIQUES (ART-THéRAPIE DE COLORIAGE)

    OpenAIRE

    KONEVA (HAAN) MARINA GENNADIEVNA

    2014-01-01

    L’article est consacré aux problématiques liées à l’acquisition des compétences lexicales à partir des textes littéraires et des œuvres de coloriage de l’art-thérapie. En s’appuyant sur l’analyse psycholinguistique du fonctionnement des émotions dans la production des images mentales des mots, on propose une méthode pédagogique, basée sur la synergie des émotions, sensations et cognition. La méthode peut être utilisée dans le cadre institutionnel (collège, lycée, université), ainsi que pour l...

  13. Vers une meilleure comprehension des savoirs disciplinaires et curriculaires des enseignants ou Ie paradigme retrouve

    Directory of Open Access Journals (Sweden)

    Stephane Martineau

    1999-01-01

    Full Text Available eet article se veut essentieUement un travail de synthese sous la forme d 'une breve revue de litterature au sujet des savoirs disciplinaires et curriculaires en enseignement. Plus specifiquement, il vise a determiner queUes sont les principales conclusions qui se degagent de la lecture des recherches empiriques sur les savoirs disciplinaires et curriculaires des enseignants des ordres d'enseignement primaire et secondaire. Il propose donc une classification des differentes dimensions constitutives du rapport entre les savoirs disciplinaires et curriculaires et la pratique enseignante. Les resultats mis au jour permettent d 'une part, d'identifier les facteurs qui entrainent des differences dans les connaissances disciplinaires et curriculaires des enseignants et d'autre part, de mieux saisir comment ces connaissances de l'enseignant peuvent influencer en retour sa pratique.

  14. Insulin receptor isoforms : an integrated view focused on gestational diabetes mellitus

    NARCIS (Netherlands)

    Westermeier, F.; Sáez, T.; Arroyo, P.; Toledo, F.; Gutierrez, J.; Sanhueza, C.; Pardo, F.; Leiva, A.; Sobrevia, L.

    2016-01-01

    The human insulin receptor (IR) exists in two isoforms that differ by the absence (IR-A) or the presence (IR-B) of a 12-amino acid segment encoded by exon 11. Both isoforms are functionally distinct regarding their binding affinities and intracellular signalling. However, the underlying mechanisms r

  15. Glial fibrillary acidic protein isoform expression in plaque related astrogliosis in Alzheimer's disease.

    Science.gov (United States)

    Kamphuis, Willem; Middeldorp, Jinte; Kooijman, Lieneke; Sluijs, Jacqueline A; Kooi, Evert-Jan; Moeton, Martina; Freriks, Michel; Mizee, Mark R; Hol, Elly M

    2014-03-01

    In Alzheimer's disease (AD), amyloid plaques are surrounded by reactive astrocytes with an increased expression of intermediate filaments including glial fibrillary acidic protein (GFAP). Different GFAP isoforms have been identified that are differentially expressed by specific subpopulations of astrocytes and that impose different properties to the intermediate filament network. We studied transcript levels and protein expression patterns of all known GFAP isoforms in human hippocampal AD tissue at different stages of the disease. Ten different transcripts for GFAP isoforms were detected at different abundancies. Transcript levels of most isoforms increased with AD progression. GFAPδ-immunopositive astrocytes were observed in subgranular zone, hilus, and stratum-lacunosum-moleculare. GFAPδ-positive cells also stained for GFAPα. In AD donors, astrocytes near plaques displayed increased staining of both GFAPα and GFAPδ. The reading-frame-shifted isoform, GFAP(+1), staining was confined to a subset of astrocytes with long processes, and their number increased in the course of AD. In conclusion, the various GFAP isoforms show differential transcript levels and are upregulated in a concerted manner in AD. The GFAP(+1) isoform defines a unique subset of astrocytes, with numbers increasing with AD progression. These data indicate the need for future exploration of underlying mechanisms concerning the functions of GFAPδ and GFAP(+1) isoforms in astrocytes and their possible role in AD pathology. Copyright © 2014 Elsevier Inc. All rights reserved.

  16. Roles of the troponin isoforms during indirect flight muscle development in Drosophila

    Indian Academy of Sciences (India)

    Salam Herojeet Singh; Prabodh Kumar; Nallur B. Ramachandra; Upendra Nongthomba

    2014-08-01

    Troponin proteins in cooperative interaction with tropomyosin are responsible for controlling the contraction of the striated muscles in response to changes in the intracellular calcium concentration. Contractility of the muscle is determined by the constituent protein isoforms, and the isoforms can switch over from one form to another depending on physiological demands and pathological conditions. In Drosophila, amajority of themyofibrillar proteins in the indirect flight muscles (IFMs) undergo post-transcriptional and post-translational isoform changes during pupal to adult metamorphosis to meet the high energy and mechanical demands of flight. Using a newly generated Gal4 strain (UH3-Gal4) which is expressed exclusively in the IFMs, during later stages of development, we have looked at the developmental and functional importance of each of the troponin subunits (troponin-I, troponin-T and troponin-C) and their isoforms. We show that all the troponin subunits are required for normal myofibril assembly and flight, except for the troponin-C isoform 1 (TnC1). Moreover, rescue experiments conducted with troponin-I embryonic isoform in the IFMs, where flies were rendered flightless, show developmental and functional differences of TnI isoforms and importance of maintaining the right isoform.

  17. Molecular cloning and pharmacology of functionally distinct isoforms of the human histamine H(3) receptor

    DEFF Research Database (Denmark)

    Wellendorph, Petrine; Goodman, M W; Burstein, E S

    2002-01-01

    The pharmacology of histamine H(3) receptors suggests the presence of distinct receptor isoforms or subtypes. We herein describe multiple, functionally distinct, alternatively spliced isoforms of the human H(3) receptor. Combinatorial splicing at three different sites creates at least six distinc...

  18. Roles of the troponin isoforms during indirect flight muscle development in Drosophila.

    Science.gov (United States)

    Singh, Salam Herojeet; Kumar, Prabodh; Ramachandra, Nallur B; Nongthomba, Upendra

    2014-08-01

    Troponin proteins in cooperative interaction with tropomyosin are responsible for controlling the contraction of the striated muscles in response to changes in the intracellular calcium concentration. Contractility of the muscle is determined by the constituent protein isoforms, and the isoforms can switch over from one form to another depending on physiological demands and pathological conditions. In Drosophila, amajority of themyofibrillar proteins in the indirect flight muscles (IFMs) undergo post-transcriptional and post-translational isoform changes during pupal to adult metamorphosis to meet the high energy and mechanical demands of flight. Using a newly generated Gal4 strain (UH3-Gal4) which is expressed exclusively in the IFMs, during later stages of development, we have looked at the developmental and functional importance of each of the troponin subunits (troponin-I, troponin-T and troponin-C) and their isoforms. We show that all the troponin subunits are required for normal myofibril assembly and flight, except for the troponin-C isoform 1 (TnC1). Moreover, rescue experiments conducted with troponin-I embryonic isoform in the IFMs, where flies were rendered flightless, show developmental and functional differences of TnI isoforms and importance of maintaining the right isoform.

  19. Development of isoform-specific sensors of polypeptide GalNAc-transferase activity

    DEFF Research Database (Denmark)

    Song, Lina; Bachert, Collin; Schjoldager, Katrine T

    2014-01-01

    Humans express up to 20 isoforms of GalNAc-transferase (herein T1-T20) that localize to the Golgi apparatus and initiate O-glycosylation. Regulation of this enzyme family affects a vast array of proteins transiting the secretory pathway and diseases arise upon misregulation of specific isoforms...

  20. Mitochondrial localization of the OAS1 p46 isoform associated with a common single nucleotide polymorphism

    DEFF Research Database (Denmark)

    Kjær, Karina Hansen; Pahus, Jytte; Hansen, Mariann Fagernæs

    2014-01-01

    vacuoles/lysosomes. By using recombinantly expressed OAS1 mutant proteins, we found that the OAS1 SNP rs1131454 (former rs3741981) did not affect the enzymatic OAS1 activity. Conclusions: The SNP rs10774671 determines differential expression of the OAS1 isoforms. In Daudi and HT1080 cells the p46 isoform...

  1. Comprehensive analysis of tropomyosin isoforms in skeletal muscles by top-down proteomics.

    Science.gov (United States)

    Jin, Yutong; Peng, Ying; Lin, Ziqing; Chen, Yi-Chen; Wei, Liming; Hacker, Timothy A; Larsson, Lars; Ge, Ying

    2016-04-01

    Mammalian skeletal muscles are heterogeneous in nature and are capable of performing various functions. Tropomyosin (Tpm) is a major component of the thin filament in skeletal muscles and plays an important role in controlling muscle contraction and relaxation. Tpm is known to consist of multiple isoforms resulting from different encoding genes and alternative splicing, along with post-translational modifications. However, a systematic characterization of Tpm isoforms in skeletal muscles is still lacking. Therefore, we employed top-down mass spectrometry (MS) to identify and characterize Tpm isoforms present in different skeletal muscles from multiple species, including swine, rat, and human. Our study revealed that Tpm1.1 and Tpm2.2 are the two major Tpm isoforms in swine and rat skeletal muscles, whereas Tpm1.1, Tpm2.2, and Tpm3.12 are present in human skeletal muscles. Tandem MS was utilized to identify the sequences of the major Tpm isoforms. Furthermore, quantitative analysis revealed muscle-type specific differences in the abundance of un-modified and modified Tpm isoforms in rat and human skeletal muscles. This study represents the first systematic investigation of Tpm isoforms in skeletal muscles, which not only demonstrates the capabilities of top-down MS for the comprehensive characterization of skeletal myofilament proteins but also provides the basis for further studies on these Tpm isoforms in muscle-related diseases.

  2. Translational control of C/EBPalpha and C/EBPbeta isoform expression

    NARCIS (Netherlands)

    Calkhoven, C F; Müller, C; Leutz, A

    2000-01-01

    Transcription factors derived from CCAAT/enhancer binding protein (C/EBP)alpha and C/EBPbeta genes control differentiation and proliferation in a number of cell types. Various C/EBP isoforms arise from unique C/EBPbeta and C/EBPalpha mRNAs by differential initiation of translation. These isoforms re

  3. Cloning and characterization of the neural isoforms of human dystonin

    Energy Technology Data Exchange (ETDEWEB)

    Brown, A.; Dalpe, G.; Mathieu, M.; Kothary, R. [Univ. of Montreal, Quebec (Canada)

    1995-10-10

    Dystonia musculorum (dt) is a hereditary neurodegenerative disease in mice that leads to a sensory ataxia. We have identified and cloned a gene encoded at the dt locus. The product of the dt gene, dystonin, is a neural isoform of a hemidesmosomal protein bullous pemphigoid antigen 1 (bpag1). To investigate the potential role of dystonin in human neuropathies, we have cloned the neural-specific 5{prime} exons of the human DT gene that together with the previously cloned BPAG1 sequences comprise human dystonin. The mouse and human dystonin genes demonstrate the same spectrum of alternatively spliced products, and the amino acid sequences of the neural-specific exons in the mouse and human genes are over 96% identical. 17 refs., 2 figs.

  4. Differences in expression, actions and cocaine regulation of two isoforms for the brain transcriptional regulator NAC1.

    Science.gov (United States)

    Korutla, L; Wang, P J; Lewis, D M; Neustadter, J H; Stromberg, M F; Mackler, S A

    2002-01-01

    BTB/POZ proteins can influence the cell cycle and contribute to oncogenesis. Many family members are present in the mammalian CNS. Previous work demonstrated elevated NAC1 mRNA levels in the rat nucleus accumbens in response to cocaine. NAC1 acts like other BTB/POZ proteins that regulate transcription but is unusual because of the absence of identifiable DNA binding domains. cDNAs were isolated encoding two NAC1 isoforms differing by only 27 amino acids (the longer isoform contains 514 amino acids). The mRNAs for both isoforms were simultaneously expressed throughout the rat brain and peripheral tissues. Semi-quantitative reverse transcription-polymerase chain reaction analysis revealed that the mRNA of the longer isoform was more abundant than the mRNA of the shorter isoform. Western blot analysis demonstrated a similar unequal distribution between the isoforms in the CNS. The longer isoform was the more abundant of the two NAC1 proteins and the ratio between them differed throughout the rat brain. The shorter isoform was not detected in most of the examined peripheral tissues, suggesting differences from the CNS in post-transcriptional processing. Both isoforms repressed transcription in H293T cells using a Gal4-luciferase reporter system. However, the shorter isoform did not repress transcription as effectively as the longer isoform. Transfection of different ratios for both isoforms, in order to replicate the relative amounts observed throughout the CNS, supported an interaction between the isoforms. The net effect on transcriptional repression was determined by the ratio of the two NAC1 isoforms. Each isoform exhibited the subnuclear localization that is characteristic of many BTB/POZ proteins. A rapid and transient increase in the level of the shorter isoform occurred in the nucleus accumbens 2 h following a single i.p. cocaine injection. We conclude that the two isoforms of NAC1 may differentially affect neuronal functions, including the regulation of

  5. Troponin T isoform expression is modulated during Atlantic Halibut metamorphosis

    Directory of Open Access Journals (Sweden)

    Llewellyn Lynda

    2007-06-01

    Full Text Available Abstract Background Flatfish metamorphosis is a thyroid hormone (TH driven process which leads to a dramatic change from a symmetrical larva to an asymmetrical juvenile. The effect of THs on muscle and in particular muscle sarcomer protein genes is largely unexplored in fish. The change in Troponin T (TnT, a pivotal protein in the assembly of skeletal muscles sarcomeres and a modulator of calcium driven muscle contraction, during flatfish metamophosis is studied. Results In the present study five cDNAs for halibut TnT genes were cloned; three were splice variants arising from a single fast TnT (fTnT gene; a fourth encoded a novel teleost specific fTnT-like cDNA (AfTnT expressed exclusively in slow muscle and the fifth encoded the teleost specific sTnT2. THs modified the expression of halibut fTnT isoforms which changed from predominantly basic to acidic isoforms during natural and T4 induced metamorphosis. In contrast, expression of red muscle specific genes, AfTnT and sTnT2, did not change during natural metamorphosis or after T4 treatment. Prior to and after metamorphosis no change in the dorso-ventral symmetry or temporal-spatial expression pattern of TnT genes and muscle fibre organization occurred in halibut musculature. Conclusion Muscle organisation in halibut remains symmetrical even after metamorphosis suggesting TH driven changes are associated with molecular adaptations. We hypothesize that species specific differences in TnT gene expression in teleosts underlies different larval muscle developmental programs which better adapts them to the specific ecological constraints.

  6. Functional Cooperativity between ABCG4 and ABCG1 Isoforms.

    Directory of Open Access Journals (Sweden)

    Zoltán Hegyi

    Full Text Available ABCG4 belongs to the ABCG subfamily, the members of which are half transporters composed of a single transmembrane and a single nucleotide-binding domain. ABCG proteins have a reverse domain topology as compared to other mammalian ABC transporters, and have to form functional dimers, since the catalytic sites for ATP binding and hydrolysis, as well as the transmembrane domains are composed of distinct parts of the monomers. Here we demonstrate that ABCG4 can form homodimers, but also heterodimers with its closest relative, ABCG1. Both the full-length and the short isoforms of ABCG1 can dimerize with ABCG4, whereas the ABCG2 multidrug transporter is unable to form a heterodimer with ABCG4. We also show that contrary to that reported in some previous studies, ABCG4 is predominantly localized to the plasma membrane. While both ABCG1 and ABCG4 have been suggested to be involved in lipid transport or regulation, in accordance with our previous results regarding the long version of ABCG1, here we document that the expression of both the short isoform of ABCG1 as well as ABCG4 induce apoptosis in various cell types. This apoptotic effect, as a functional read-out, allowed us to demonstrate that the dimerization between these half transporters is not only a physical interaction but functional cooperativity. Given that ABCG4 is predominantly expressed in microglial-like cells and endothelial cells in the brain, our finding of ABCG4-induced apoptosis may implicate a new role for this protein in the clearance mechanisms within the central nervous system.

  7. Catégorisation aspectuelle des concepts

    Directory of Open Access Journals (Sweden)

    Karolak Stanisław

    2008-01-01

    Full Text Available (francuski Le contenu de l'article est le problème connu en aspectologie comme celui de la dépendance entre le sens lexical des verbes et l'aspect. Les contraintes qu'imposent les langues à la distribution des grammèmes d'aspect ont fait supposer qu'il existe des règles de leur co-occurrence avec les sémantèmes. Le problème est posé ici dans une perspective conceptuelle. Dans cette perspective, les verbes dont les sémantèmes ont tel ou tel aspect n'exigent pas la co-occurrence avec des grammèmes qui véhiculent le même aspect. En revanche, ils peuvent se combiner avec des grammèmes de sens aspectuel opposé pour dériver des catégories de verbes secondaires à partir des catégories primaires. On postule l'existence d'un mécanisme dérivationnel qui engendre des verbes polyaspectuels.

  8. Supply Chain et Gestion des ressources humaines

    OpenAIRE

    Nondonfaz, Robert

    2010-01-01

    Rappels de quelques exigences de la Supply Chain en matière de gestion des ressources humaines; spécificités de certains métiers : chauffeur, magasinier, acheteur, gestionnaire de stock, gestion des imprévus

  9. internationalisation et nouvelles implantations des firmes ...

    African Journals Online (AJOL)

    Administrateur

    de disparités culturelles ou dans les pratiques des affaires ou encore dans le .... plus pertinents afin d'expliquer l'évolution du modèle (cas de la distance psychique par ..... Elles constituent plutôt une lecture globale et intégrée des processus.

  10. La tuberculose des amygdales palatines

    Directory of Open Access Journals (Sweden)

    Oudidi Abdelatif

    2010-01-01

    Full Text Available INTRODUCTION:La localisation tuberculeuse des amygdales palatines est très rare même dans un pays d’endémie tuberculeuse. Le diagnostic peut être suspecté cliniquement dans un contexte évocateur, mais il ne peut être certifié qu’au stade histologique. PATIENTS ET METHODES:Cinq cas de tuberculose des amygdales palatine ont été diagnostiqués sur une période de 5 ans et inclus dans une étude rétrospective. Nous avons recensé trois hommes et deux femmes (âge moyen de 28 ans. La dysphagie haute était le maître symptôme. L’examen a objectivé une hypertrophie amygdalienne chez tous les patients avec des ulcérations chez trois d’entre eux. Une biopsie a été effectuée chez trois patients et une amygdalectomie a été réalisée chez les deux autres. L’étude anatomopathologique a posé le diagnostic chez tous les patients. Un traitement médical antituberculeux selon un régime court allant de 6 à 9 mois a été instauré. Dans tous les cas, l’évolution à long terme a été favorable avec un recul moyen de 13 mois. CONCLUSION: La tuberculose amygdalienne est rare. Son diagnostic repose essentiellement sur le résultat anatomopathologique de la biopsie où de l’amygdalectomie. L’évolution sous traitement anti-bacillaire est souvent favorable, la récidive est exceptionnelle.

  11. Schopenhauer und die Paradoxien des Erhabenen

    Directory of Open Access Journals (Sweden)

    François Arnaud

    2012-08-01

    Full Text Available http://dx.doi.org/10.5007/1677-2954.2012v11nesp1p145Die Hauptunterscheidung zwischen den Schopenhauerschen und Kantschen Auffassungen des Erhabenen liegt nach diesem Artikel darin, dass der Zuschauer im letzten Fall zu einer nur möglichen Gefahr muss unterworfen werden, während er im Ersten wirklich erschreckt sein muss. Die Haupttriebfeder des Gefühls des Erhabenen ist nämlich bei Schopenhauer der Einsatz des Willens, der also wirklich bedroht werden muss. Daraus folgen einige Paradoxien und Originalitäten der Schopenhauerschen Lehre des Erhabenen, hauptsächlich aus dem ethischen Aussichtspunkt, deren Spuren ich zu folgen versuche.

  12. Improved Mask Protected DES using RSA Algorithm

    Directory of Open Access Journals (Sweden)

    Asha Latha S.

    2016-01-01

    Full Text Available The data encryption standard is a pioneering and farsighted standard which helped to set a new paradigm for encryption standards. But now DES is considered to be insecure for some application. Asymmetric mask protected DES is an advanced encryption method for effectively protecting the advanced DES. There are still probabilities to improve its security. This paper propose a method, which introduce a RSA key generation scheme in mask protected DES instead of plain key, which result in enhancement in the security of present asymmetric mask protected DES. We further propose a Vedic mathematical method of RSA implementation which reduce the complexity of computation in RSA block thereby resulting in reduced delay (four timesthat improves the performance of overall system. The software implementation was performed using Xilinx 13.2 and Model-Sim was used for the simulation environment.

  13. Characterization of ductal and lobular breast carcinomas using novel prolactin receptor isoform specific antibodies

    Directory of Open Access Journals (Sweden)

    Heger Christopher D

    2010-12-01

    Full Text Available Abstract Background Prolactin is a polypeptide hormone responsible for proliferation and differentiation of the mammary gland. More recently, prolactin's role in mammary carcinogenesis has been studied with greater interest. Studies from our laboratory and from others have demonstrated that three specific isoforms of the prolactin receptor (PRLR are expressed in both normal and cancerous breast cells and tissues. Until now, reliable isoform specific antibodies have been lacking. We have prepared and characterized polyclonal antibodies against each of the human PRLR isoforms that can effectively be used to characterize human breast cancers. Methods Rabbits were immunized with synthetic peptides of isoform unique regions and immune sera affinity purified prior to validation by Western blot and immunohistochemical analyses. Sections of ductal and lobular carcinomas were stained with each affinity purified isoform specific antibody to determine expression patterns in breast cancer subclasses. Results We show that the rabbit antibodies have high titer and could specifically recognize each isoform of PRLR. Differences in PRLR isoform expression levels were observed and quantified using histosections from xenografts of established human breast cancer cells lines, and ductal and lobular carcinoma human biopsy specimens. In addition, these results were verified by real-time PCR with isoform specific primers. While nearly all tumors contained LF and SF1b, the majority (76% of ductal carcinoma biopsies expressed SF1a while the majority of lobular carcinomas lacked SF1a staining (72% and 27% had only low levels of expression. Conclusions Differences in the receptor isoform expression profiles may be critical to understanding the role of PRL in mammary tumorigenesis. Since these antibodies are specifically directed against each PRLR isoform, they are valuable tools for the evaluation of breast cancer PRLR content and have potential clinical importance in

  14. Des fantômes

    Directory of Open Access Journals (Sweden)

    Sjef Houppermans

    2012-12-01

    Full Text Available La première pièce de théâtre de Laurent Mauvignier, Tout mon amour, est créée en 2012 par la compagnie Les Possédés. La thématique habituelle de Mauvignier (secrets des familles, solitude, violence sociale s’y retrouve, mais tout en utilisant pleinement les moyens de la scène (distanciation, présence physique, espace théâtral, discours rythmés.

  15. Des pauvres de la politique à la politique des pauvres

    Directory of Open Access Journals (Sweden)

    Ratiba Hadj-Moussa

    2012-01-01

    Full Text Available S’attachant aux nouvelles modalités de production du politique issues des études subalternes, l’article situe dans un premier temps ces travaux et, dans un deuxième temps, en fait une présentation en s’appuyant sur les éléments qui y sont privilégiés et qui font le lien entre « les pauvres et la production du politique ». Cette présentation s’appuie essentiellement sur les travaux portant sur les pays du Sud, en particulier l’Inde et quelques pays du Moyen Orient (l’Égypte et l’Iran. Elle réfère aussi au Maghreb qui est le terrain principal de l’analyse. La présentation tient compte en particulier des travaux qui s’appuient sur des recherches empiriques substantielles mais dont la portée théorique est très peu discutée. Enfin, si le renvoi aux études subalternes ou postcoloniales est dans ce texte non négligeable, il est aussi fait recours à des travaux produits dans des contextes pluralistes, comme la France.Poor Policy to the Policy of the PoorThis article has its origins in the new methods of production of policy based on subaltern studies. Firstly, it will locate these studies and then it will present its main arguments as well as the relationship between “the poor and policy production”. This presentation is based essentially on the works on countries of the south, particularly India, and few other countries from the Middle East (Egypt and Iran. It also refers to North Africa, which is the major fieldwork of this analysis. This presentation takes into account the works that rely upon substantial empirical research but for which the theoretical range is very little. Finally, if the referral to subaltern or postcolonial studies is significant in this article, it also refers to works produced in pluralistic contexts such as France.De los pobres de la política a la política de los pobresBasándose en estudios subalternos, el artículo se sitúa dentro del marco de los nuevos modos de producci

  16. Évaluation des émissions de gaz à effet de serre des filières de traitement et de valorisation des boues issues du traitement des eaux usées

    OpenAIRE

    Reverdy, A.L.; Pradel, M.

    2011-01-01

    / Ce rapport présente l'inventaire des différentes consommations énergétiques, en consommables et des GES pour chaque poste de traitement et de valorisation des boues issues du traitement des eaux usées.

  17. Distribution spatiale intra-urbaine des particules fines : monitoring ...

    African Journals Online (AJOL)

    SARAH

    30 sept. 2014 ... Intra-urban spatial distribution of particulate matter: monitoring by leaf Saturation Isothermal Remanent ... les changements microclimatiques au sein des villes ... déterminer l'impact des saisons sur la teneur des ... Ce District jouit d'un climat de type ... Sélection et description des espèces : Un inventaire.

  18. Performances de production des cobayes (Cavia porcellus L.) en ...

    African Journals Online (AJOL)

    Au Cameroun, l'élevage des cobayes se pratique encore dans les cuisines et à même le sol et leur ..... Figure 1 : Evolution des poids des mères allaitantes de la naissance au sevrage des petits. .... lecture critique du manuscrit ; EM a participé.

  19. Dystrophin Dp71 Isoforms Are Differentially Expressed in the Mouse Brain and Retina: Report of New Alternative Splicing and a Novel Nomenclature for Dp71 Isoforms.

    Science.gov (United States)

    Aragón, Jorge; González-Reyes, Mayram; Romo-Yáñez, José; Vacca, Ophélie; Aguilar-González, Guadalupe; Rendón, Alvaro; Vaillend, Cyrille; Montañez, Cecilia

    2017-01-27

    Multiple dystrophin Dp71 isoforms have been identified in rats, mice, and humans and in several cell line models. These Dp71 isoforms are produced by the alternative splicing of exons 71 to 74 and 78 and intron 77. Three main groups of Dp71 proteins are defined based on their C-terminal specificities: Dp71d, Dp71f, and Dp71e. Dp71 is highly expressed in the brain and retina; however, the specific isoforms present in these tissues have not been determined to date. In this work, we explored the expression of Dp71 isoforms in the mouse brain and retina using RT-PCR assays followed by the cloning of PCR products into the pGEM-T Easy vector, which was used to transform DH5α cells. Dp71-positive colonies were later analyzed by PCR multiplex and DNA sequencing to determine the alternative splicing. We thus demonstrated the expression of Dp71 transcripts corresponding to Dp71, Dp71a, Dp71c, Dp71b, Dp71ab, Dp71 Δ110, and novel Dp71 isoforms spliced in exon 74; 71 and 74; 71, 73 and 74; and 74 and 78, which we named Dp71d Δ74 , Dp71d Δ71,74 , Dp71d Δ71,73-74 , and Dp71f Δ74 , respectively. Additionally, we demonstrated that the Dp71d group of isoforms is highly expressed in the brain, while the Dp71f group predominates in the retina, at both the cDNA and protein levels. These findings suggest that distinct Dp71 isoforms may play different roles in the brain and retina.

  20. Isomers chart; Table des isomeres

    Energy Technology Data Exchange (ETDEWEB)

    Dupont-Gautier, P.; Chantelot, S.; Moisson, N. [Commissariat a l' Energie Atomique, Saclay (France). Centre d' Etudes Nucleaires

    1967-07-01

    The nuclear isomers are nuclides offering the same mass number and the same atomic number, but different energy levels. In the following chart the zero energy ground states are omitted and the metastable isomers, i.e. of non-zero energy, known and of measurable lifetime, are listed. The lower limit of this lifetime was set here to 0.1 x 10{sup -6} s. The various isomers were classified in increasing lifetimes. (authors) [French] Les isomeres nucleaires sont des nucleides presentant le meme nombre de masse et le meme numero atomique, mais des niveaux energetiques differents. Dans la table suivante, on a neglige les etats fondamentaux d'energie nulle et on a recense les isomeres metastables, c'est-a-dire d'energie non nulle, connus et de periode mesurable. La limite inferieure de cette periode a ete fixee ici a 0,1 x 10{sup -6} s. Les differents isomeres ont ete classes par periodes croissantes. (auteurs)

  1. Isomers chart; Table des isomeres

    Energy Technology Data Exchange (ETDEWEB)

    Dupont-Gautier, P.; Chantelot, S.; Moisson, N. [Commissariat a l' Energie Atomique, Saclay (France). Centre d' Etudes Nucleaires

    1967-07-01

    The nuclear isomers are nuclides offering the same mass number and the same atomic number, but different energy levels. In the following chart the zero energy ground states are omitted and the metastable isomers, i.e. of non-zero energy, known and of measurable lifetime, are listed. The lower limit of this lifetime was set here to 0.1 x 10{sup -6} s. The various isomers were classified in increasing lifetimes. (authors) [French] Les isomeres nucleaires sont des nucleides presentant le meme nombre de masse et le meme numero atomique, mais des niveaux energetiques differents. Dans la table suivante, on a neglige les etats fondamentaux d'energie nulle et on a recense les isomeres metastables, c'est-a-dire d'energie non nulle, connus et de periode mesurable. La limite inferieure de cette periode a ete fixee ici a 0,1 x 10{sup -6} s. Les differents isomeres ont ete classes par periodes croissantes. (auteurs)

  2. Serum amyloid A isoforms in serum and synovial fluid from spontaneously diseased dogs with joint diseases or other conditions

    DEFF Research Database (Denmark)

    Kjelgaard-Hansen, Mads Jens; Christensen, Michelle B.; Lee, Marcel Huisung

    2007-01-01

    with systemic inflammatory activity, and up to four major isoforms with apparent isoelectric points between 6.1 and 7.9 were identified. In synovial fluid from inflamed joints one or more highly alkaline SAA isoforms (with apparent isoelectric points above 9.3) were identified, with data suggesting local...... production of these isoforms in the canine inflamed joint....

  3. Merlin Isoforms 1 and 2 Both Act as Tumour Suppressors and Are Required for Optimal Sperm Maturation.

    Directory of Open Access Journals (Sweden)

    Ansgar Zoch

    Full Text Available The tumour suppressor Merlin, encoded by the gene NF2, is frequently mutated in the autosomal dominant disorder neurofibromatosis type II, characterised primarily by the development of schwannoma and other glial cell tumours. However, NF2 is expressed in virtually all analysed human and rodent organs, and its deletion in mice causes early embryonic lethality. Additionally, NF2 encodes for two major isoforms of Merlin of unknown functionality. Specifically, the tumour suppressor potential of isoform 2 remains controversial. In this study, we used Nf2 isoform-specific knockout mouse models to analyse the function of each isoform during development and organ homeostasis. We found that both isoforms carry full tumour suppressor functionality and can completely compensate the loss of the other isoform during development and in most adult organs. Surprisingly, we discovered that spermatogenesis is strictly dependent on the presence of both isoforms. While the testis primarily expresses isoform 1, we noticed an enrichment of isoform 2 in spermatogonial stem cells. Deletion of either isoform was found to cause decreased sperm quality as observed by maturation defects and head/midpiece abnormalities. These defects led to impaired sperm functionality as assessed by decreased sperm capacitation. Thus, we describe spermatogenesis as a new Nf2-dependent process. Additionally, we provide for the first time in vivo evidence for equal tumour suppressor potentials of Merlin isoform 1 and isoform 2.

  4. Etude des effets du martelage repetitif sur les contraintes residuelles

    Science.gov (United States)

    Hacini, Lyes

    L'assemblage par soudage peut engendrer des contraintes residuelles. Ces contraintes provoquent des fissurations prematurees et un raccourcissement de la duree de vie des composants. Dans ce contexte, le martelage robotise est utilise pour relaxer ces contraintes residuelles. Trois volets sont presentes: le premier est l'evaluation des effets des impacts unitaires repetes sur le champ de contraintes developpe dans des plaques d'acier inoxydable austenitique 304L vierges ou contenant des contraintes residuelles initiales. Dans la deuxieme partie de ce projet, le martelage est applique grace au robot SCOMPI. Les contraintes residuelles induites et relaxees par martelage sont ensuite mesurees par la methode des contours, qui a ete adaptee a cet effet. Dans la troisieme partie, le martelage est modelise par la methode des elements finis. Un modele axisymetrique developpe grace au logiciel ANSYS permet de simuler des impacts repetes d'un marteau elastique sur une plaque ayant un comportement elastoplastique.

  5. Role of nuclear progesterone receptor isoforms in uterine pathophysiology.

    Science.gov (United States)

    Patel, Bansari; Elguero, Sonia; Thakore, Suruchi; Dahoud, Wissam; Bedaiwy, Mohamed; Mesiano, Sam

    2015-01-01

    Progesterone is a key hormonal regulator of the female reproductive system. It plays a major role to prepare the uterus for implantation and in the establishment and maintenance of pregnancy. Actions of progesterone on the uterine tissues (endometrium, myometrium and cervix) are mediated by the combined effects of two progesterone receptor (PR) isoforms, designated PR-A and PR-B. Both receptors function primarily as ligand-activated transcription factors. Progesterone action on the uterine tissues is qualitatively and quantitatively determined by the relative levels and transcriptional activities of PR-A and PR-B. The transcriptional activity of the PR isoforms is affected by specific transcriptional coregulators and by PR post-translational modifications that affect gene promoter targeting. In this context, appropriate temporal and cell-specific expression and function of PR-A and PR-B are critical for normal uterine function. Relevant studies describing the role of PRs in uterine physiology and pathology (endometriosis, uterine leiomyoma, endometrial cancer, cervical cancer and recurrent pregnancy loss) were comprehensively searched using PubMed, Cochrane Library, Web of Science, and Google Scholar and critically reviewed. Progesterone, acting through PR-A and PR-B, regulates the development and function of the endometrium and induces changes in cells essential for implantation and the establishment and maintenance of pregnancy. During pregnancy, progesterone via the PRs promotes myometrial relaxation and cervical closure. Withdrawal of PR-mediated progesterone signaling triggers menstruation and parturition. PR-mediated progesterone signaling is anti-mitogenic in endometrial epithelial cells, and as such, mitigates the tropic effects of estrogen on eutopic normal endometrium, and on ectopic implants in endometriosis. Similarly, ligand-activated PRs function as tumor suppressors in endometrial cancer cells through inhibition of key cellular signaling pathways

  6. Dynamic expression and localization of c-MET isoforms in the developing rat pancreas.

    Science.gov (United States)

    Wu, Yulong; Cheng, Mei; Shi, Zhen; Feng, Zhenqing; Guan, Xiaohong

    2014-01-01

    Pancreata from Sprague Dawley rats of different developmental stages were studied to determine the expression and cellular localization of different c-MET isoforms in the developing rat pancreas. Pancreatic mRNA and protein expression levels of c-MET at different developmental stages from embryo to adult were detected by reverse transcription-polymerase chain reaction and by western blotting. To identify the cellular localization of c-MET protein in the developing rat pancreas, double immunofluorescent staining was performed using antibodies for cell type-specific markers and for c-MET. The expression of two isoforms of c-MET (190 kDa and 170 kDa) coincided with the development of the pancreas. The 190 kDa isoform of c-MET is expressed during embryonic stages, and its expression is replaced by the expression of the 170 kDa isoform as the pancreas develops. Only the 170 kDa isoform is expressed in the adult rat pancreas. Throughout all stages of pancreatic development, c-MET is expressed by vimentin-positive cells. In contrast, c-MET staining was stronger in rat pancreata from newborn to adult stages and overlapped with insulin-positive beta-cells. The dynamic expression and localization of different c-MET isoforms in the rat pancreas during different developmental stages indicates that distinct c-MET isoform might be involved in different aspects of pancreatic development.

  7. Distinct and shared transcriptomes are regulated by microphthalmia-associated transcription factor isoforms in mast cells.

    Science.gov (United States)

    Shahlaee, Amir H; Brandal, Stephanie; Lee, Youl-Nam; Jie, Chunfa; Takemoto, Clifford M

    2007-01-01

    The Microphthalmia-associated transcription factor (Mitf) is an essential basic helix-loop-helix leucine zipper transcription factor for mast cell development. Mice deficient in Mitf harbor a severe mast cell deficiency, and Mitf-mutant mast cells cultured ex vivo display a number of functional defects. Therefore, an understanding of the genetic program regulated by Mitf may provide important insights into mast cell differentiation. Multiple, distinct isoforms of Mitf have been identified in a variety of cell types; we found that Mitf-a, Mitf-e, and Mitf-mc were the major isoforms expressed in mast cells. To determine the physiologic function of Mitf in mast cells, we restored expression of these isoforms in primary mast cells from Mitf(-/-) mice. We found that these isoforms restored granular morphology and integrin-mediated migration. By microarray analysis, proteases, signaling molecules, cell surface receptor, and transporters comprised the largest groups of genes up-regulated by all isoforms. Furthermore, we found that isoforms also regulated distinct genes sets, suggesting separable biological activities. This work defines the transcriptome regulated by Mitf in mast cells and supports its role as master regulator of mast cell differentiation. Expression of multiple isoforms of this transcription factor may provide for redundancy of biological activities while also allowing diversity of function.

  8. VEGF-A isoforms program differential VEGFR2 signal transduction, trafficking and proteolysis

    Science.gov (United States)

    Fearnley, Gareth W.; Smith, Gina A.; Abdul-Zani, Izma; Yuldasheva, Nadira; Mughal, Nadeem A.; Homer-Vanniasinkam, Shervanthi; Kearney, Mark T.; Zachary, Ian C.; Tomlinson, Darren C.; Harrison, Michael A.; Wheatcroft, Stephen B.; Ponnambalam, Sreenivasan

    2016-01-01

    ABSTRACT Vascular endothelial growth factor A (VEGF-A) binding to the receptor tyrosine kinase VEGFR2 triggers multiple signal transduction pathways, which regulate endothelial cell responses that control vascular development. Multiple isoforms of VEGF-A can elicit differential signal transduction and endothelial responses. However, it is unclear how such cellular responses are controlled by isoform-specific VEGF-A–VEGFR2 complexes. Increasingly, there is the realization that the membrane trafficking of receptor–ligand complexes influences signal transduction and protein turnover. By building on these concepts, our study shows for the first time that three different VEGF-A isoforms (VEGF-A165, VEGF-A121 and VEGF-A145) promote distinct patterns of VEGFR2 endocytosis for delivery into early endosomes. This differential VEGFR2 endocytosis and trafficking is linked to VEGF-A isoform-specific signal transduction events. Disruption of clathrin-dependent endocytosis blocked VEGF-A isoform-specific VEGFR2 activation, signal transduction and caused substantial depletion in membrane-bound VEGFR1 and VEGFR2 levels. Furthermore, such VEGF-A isoforms promoted differential patterns of VEGFR2 ubiquitylation, proteolysis and terminal degradation. Our study now provides novel insights into how different VEGF-A isoforms can bind the same receptor tyrosine kinase and elicit diverse cellular outcomes. PMID:27044325

  9. VEGF-A isoforms program differential VEGFR2 signal transduction, trafficking and proteolysis

    Directory of Open Access Journals (Sweden)

    Gareth W. Fearnley

    2016-05-01

    Full Text Available Vascular endothelial growth factor A (VEGF-A binding to the receptor tyrosine kinase VEGFR2 triggers multiple signal transduction pathways, which regulate endothelial cell responses that control vascular development. Multiple isoforms of VEGF-A can elicit differential signal transduction and endothelial responses. However, it is unclear how such cellular responses are controlled by isoform-specific VEGF-A–VEGFR2 complexes. Increasingly, there is the realization that the membrane trafficking of receptor–ligand complexes influences signal transduction and protein turnover. By building on these concepts, our study shows for the first time that three different VEGF-A isoforms (VEGF-A165, VEGF-A121 and VEGF-A145 promote distinct patterns of VEGFR2 endocytosis for delivery into early endosomes. This differential VEGFR2 endocytosis and trafficking is linked to VEGF-A isoform-specific signal transduction events. Disruption of clathrin-dependent endocytosis blocked VEGF-A isoform-specific VEGFR2 activation, signal transduction and caused substantial depletion in membrane-bound VEGFR1 and VEGFR2 levels. Furthermore, such VEGF-A isoforms promoted differential patterns of VEGFR2 ubiquitylation, proteolysis and terminal degradation. Our study now provides novel insights into how different VEGF-A isoforms can bind the same receptor tyrosine kinase and elicit diverse cellular outcomes.

  10. Estrogen and progesterone receptor isoforms expression in the stomach of Mongolian gerbils

    Institute of Scientific and Technical Information of China (English)

    Milena Saqui-Salces; Teresa Neri-Gómez; Armando Gamboa-Dominguez; Guillermo Ruiz-Palacios; Ignacio Camacho-Arroyo

    2008-01-01

    AIM:We studied the estrogen receptor (ER) and progesterone receptor (PR) isoforms expression in gastric antrum and corpus of female gerbils and their regulation by estradiol (E2) and progesterone (P4).METHODS: Ovariectomized adult female gerbils were subcutaneously treated with E2,and E2 + P4.Uteri and stomachs were removed,the latter were cut along the greater curvature,and antrum and corpus were excised.Proteins were immunoblotted using antibodies that recognize ER-alpha,ER-beta,and PR-A and PR-B receptor isoforms.Tissues from rats treated in the same way were used as controls.RESULTS: Specific bands were detected for ER-alpha (68 KDa),and PR isoforms (85 and 120 KDa for PR-A and PR-B isoforrns,respectively) in uteri,gastric antrum and corpus.We could not detect ER-beta isoform.PR isoforms were not regulated by E2 or P4 in uterus and gastric tissues of gerbils.ER-alpha isoform content was significantly down-regulated by E2 in the corpus,but not affected by hormones in uterus and gastric antrum.CONCLUSION: The presence of ER-alpha and PR isoforms in gerbils stomach suggests that E2 and P4 actions in this organ are in part mediated by their nuclear receptors.

  11. Identification and evolutionary analysis of tissue-specific isoforms of mitochondrial complex I subunit NDUFV3.

    Science.gov (United States)

    Guerrero-Castillo, Sergio; Cabrera-Orefice, Alfredo; Huynen, Martijn A; Arnold, Susanne

    2017-03-01

    Mitochondrial complex I is the largest respiratory chain complex. Despite the enormous progress made studying its structure and function in recent years, potential regulatory roles of its accessory subunits remained largely unresolved. Complex I gene NDUFV3, which occurs in metazoa, contains an extra exon that is only present in vertebrates and thereby evolutionary even younger than the rest of the gene. Alternative splicing of this extra exon gives rise to a short NDUFV3-S and a long NDUFV3-L protein isoform. Complexome profiling revealed that the two NDUFV3 isoforms are constituents of the multi-subunit complex I. Further mass spectrometric analyses of complex I from different murine and bovine tissues showed a tissue-specific expression pattern of NDUFV3-S and NDUFV3-L. Hence, NDUFV3-S was identified as the only isoform in heart and skeletal muscle, whereas in liver, brain, and lung NDUFV3-L was expressed as the dominant isoform, together with NDUFV3-S present in all tissues analyzed. Thus, we identified NDUFV3 as the first out of 30 accessory subunits of complex I present in vertebrate- and tissue-specific isoforms. Interestingly, the tissue-specific expression pattern of NDUFV3-S and NDUFV3-L isoforms was paralleled by changes in kinetic parameters, especially the substrate affinity of complex I. This may indicate a regulatory role of the NDUFV3 isoforms in different vertebrate tissues.

  12. Revisiting the identification of canonical splice isoforms through integration of functional genomics and proteomics evidence.

    Science.gov (United States)

    Li, Hong-Dong; Menon, Rajasree; Omenn, Gilbert S; Guan, Yuanfang

    2014-12-01

    Canonical isoforms in different databases have been defined as the most prevalent, most conserved, most expressed, longest, or the one with the clearest description of domains or posttranslational modifications. In this article, we revisit these definitions of canonical isoforms based on functional genomics and proteomics evidence, focusing on mouse data. We report a novel functional relationship network-based approach for identifying the highest connected isoforms (HCIs). We show that 46% of these HCIs are not the longest transcripts. In addition, this approach revealed many genes that have more than one highly connected isoforms. Averaged across 175 RNA-seq datasets covering diverse tissues and conditions, 65% of the HCIs show higher expression levels than nonhighest connected isoforms at the transcript level. At the protein level, these HCIs highly overlap with the expressed splice variants, based on proteomic data from eight different normal tissues. These results suggest that a more confident definition of canonical isoforms can be made through integration of multiple lines of evidence, including HCIs defined by biological processes and pathways, expression prevalence at the transcript level, and relative or absolute abundance at the protein level. This integrative proteogenomics approach can successfully identify principal isoforms that are responsible for the canonical functions of genes. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  13. Channel properties of the splicing isoforms of the olfactory calcium-activated chloride channel Anoctamin 2.

    Science.gov (United States)

    Ponissery Saidu, Samsudeen; Stephan, Aaron B; Talaga, Anna K; Zhao, Haiqing; Reisert, Johannes

    2013-06-01

    Anoctamin (ANO)2 (or TMEM16B) forms a cell membrane Ca(2+)-activated Cl(-) channel that is present in cilia of olfactory receptor neurons, vomeronasal microvilli, and photoreceptor synaptic terminals. Alternative splicing of Ano2 transcripts generates multiple variants with the olfactory variants skipping exon 14 and having alternative splicing of exon 4. In the present study, 5' rapid amplification of cDNA ends analysis was conducted to characterize the 5' end of olfactory Ano2 transcripts, which showed that the most abundant Ano2 transcripts in the olfactory epithelium contain a novel starting exon that encodes a translation initiation site, whereas transcripts of the publically available sequence variant, which has an alternative and longer 5' end, were present in lower abundance. With two alternative starting exons and alternative splicing of exon 4, four olfactory ANO2 isoforms are thus possible. Patch-clamp experiments in transfected HEK293T cells expressing these isoforms showed that N-terminal sequences affect Ca(2+) sensitivity and that the exon 4-encoded sequence is required to form functional channels. Coexpression of the two predominant isoforms, one with and one without the exon 4 sequence, as well as coexpression of the two rarer isoforms showed alterations in channel properties, indicating that different isoforms interact with each other. Furthermore, channel properties observed from the coexpression of the predominant isoforms better recapitulated the native channel properties, suggesting that the native channel may be composed of two or more splicing isoforms acting as subunits that together shape the channel properties.

  14. Androgen receptor isoforms in human prostatic cancer tissue and LNCaP cell line

    Institute of Scientific and Technical Information of China (English)

    Shu-Jie XIA; Xiao-Da TANG; Qing-Zheng MA

    2001-01-01

    Aim: To investigate the androgen receptor (AR) isoform expressions in human prostatic cancer tissue and LNCaP cell line. Methods: With high resolution isoelectric focusing (IEF) method we demonstrated the different expressions of AR isoforms in human prostatic cancer tissues and LNCaP cell line. Results: Data were obtained from three prostatic cancer specimens and the LNCaP cell line. Three types of AR isoforms were detected with pI values at 6.5,6.0, and 5.3. For the 3 prostatic cancer specimens, 1 sample showed all the three types of AR isoforms, the second specimen expressed at 6.5 and 6.0, and the third failed to show any type of isoforms. The LNCaP cell line expressed all the three AR isoforms. Binding of 3H-dihydrotestosterone (3H-DHT) to these three isoforms was inhibited by the addition ofl00-fold excess of DHT or testosterone, while not by progesterone, oestradiol and diethylstilboestrol. Conclusion: The expression of AR isofonns is different in different prostate cancer tissues, which may be related to the difference in the effect of anti-androgen therapy in different patients.

  15. Differential subcellular distribution of four phospholipase C isoforms and secretion of GPI-PLC activity.

    Science.gov (United States)

    Staudt, Emanuel; Ramasamy, Pathmanaban; Plattner, Helmut; Simon, Martin

    2016-12-01

    Phospholipase C (PLC) is an important enzyme of signal transduction pathways by generation of second messengers from membrane lipids. PLCs are also indicated to cleave glycosylphosphatidylinositol (GPI)-anchors of surface proteins thus releasing these into the environment. However, it remains unknown whether this enzymatic activity on the surface is due to distinct PLC isoforms in higher eukaryotes. Ciliates have, in contrast to other unicellular eukaryotes, multiple PLC isoforms as mammals do. Thus, Paramecium represents a perfect model to study subcellular distribution and potential surface activity of PLC isoforms. We have identified distinct subcellular localizations of four PLC isoforms indicating functional specialization. The association with different calcium release channels (CRCs) argues for distinct subcellular functions. They may serve as PI-PLCs in microdomains for local second messenger responses rather than free floating IP3. In addition, all isoforms can be found on the cell surface and they are found together with GPI-cleaved surface proteins in salt/ethanol washes of cells. We can moreover show them in medium supernatants of living cells where they have access to GPI-anchored surface proteins. Among the isoforms we cannot assign GPI-PLC activity to specific PLC isoforms; rather each PLC is potentially responsible for the release of GPI-anchored proteins from the surface. Copyright © 2016 Elsevier B.V. All rights reserved.

  16. Inulin isoforms differ by repeated additions of one crystal unit cell.

    Science.gov (United States)

    Cooper, Peter D; Barclay, Thomas G; Ginic-Markovic, Milena; Gerson, Andrea R; Petrovsky, Nikolai

    2014-03-15

    Inulin isoforms, especially delta inulin, are important biologically as immune activators and clinically as vaccine adjuvants. In exploring action mechanisms, we previously found regular increments in thermal properties of the seven-member inulin isoform series that suggested regular additions of some energetic structural unit. Because the previous isolates carried additional longer chains that masked defining ranges, these were contrasted with new isoform isolates comprising only inulin chain lengths defining that isoform. The new series began with 19 fructose units per chain (alpha-1 inulin), increasing regularly by 6 fructose units per isoform. Thus the 'energetic unit' equates to 6 fructose residues per chain. All isoforms showed indistinguishable X-ray diffraction patterns that were also identical with known inulin crystals. We conclude that an 'energetic unit' equates to one helix turn of 6 fructose units per chain as found in one unit cell of the inulin crystal. Each isoform chain comprised progressively more helix turns plus one additional fructose and glucose residues per chain.

  17. À l’aube des dieux

    Directory of Open Access Journals (Sweden)

    Pascal Semonsut

    2011-03-01

    Full Text Available De l’avis même des préhistoriens, la Préhistoire assiste à la naissance du sentiment religieux. Cette religion des origines, quelle image en ont les Français ? Telle est la question que cet article aborde à partir de l’étude des manuels scolaires et des œuvres de fiction (littérature, cinéma et bande dessinée de la seconde moitié du XXe siècle. Si l’enseignement et la fiction n’accordent pas la même place à ce thème, en revanche tous deux font des préhistoriques des polythéistes adorant le soleil, construisant des mégalithes et prenant le plus grand soin de leurs morts. Ce mélange d’obscurantisme et de vraie spiritualité n’a qu’un but : montrer que notre espèce ne pouvait être que victorieuse dans ce combat qu’est l’humanisation.

  18. 75 caracteristiques et evolution des operations de fusions ...

    African Journals Online (AJOL)

    Nous décrivons dans un premier temps l'évolution de l'économie tunisienne et ..... grossistes, a réalisé en 2004 près de 88 % du chiffre d'affaires des transactions .... d'actions reste une opération soumise au droit commun des contrats et au droit des ..... tairement des opérations horizontales mettant en relation des entrepri-.

  19. L'espace mondial des grandes manifestations sportives internationales

    Directory of Open Access Journals (Sweden)

    Daniel MATHIEU

    1989-06-01

    Full Text Available La carte des grandes manifestations sportives mondiales montre une forte concentration des épreuves dans un petit nombre de pays développés, en particulier l'Europe occidentale. L'examen des nations concernées par les tournois majeurs du tennis et par le cyclisme professionnel soulignent, avec les nuances propres à chacun des sports, l'étroitesse des espaces intéressés.

  20. De la manipulation des images 3D

    Directory of Open Access Journals (Sweden)

    Geneviève Pinçon

    2012-04-01

    Full Text Available Si les technologies 3D livrent un enregistrement précis et pertinent des graphismes pariétaux, elles offrent également des applications particulièrement intéressantes pour leur analyse. À travers des traitements sur nuage de points et des simulations, elles autorisent un large éventail de manipulations touchant autant à l’observation qu’à l’étude des œuvres pariétales. Elles permettent notamment une perception affinée de leur volumétrie, et deviennent des outils de comparaison de formes très utiles dans la reconstruction des chronologies pariétales et dans l’appréhension des analogies entre différents sites. Ces outils analytiques sont ici illustrés par les travaux originaux menés sur les sculptures pariétales des abris du Roc-aux-Sorciers (Angles-sur-l’Anglin, Vienne et de la Chaire-à-Calvin (Mouthiers-sur-Boëme, Charente.If 3D technologies allow an accurate and relevant recording of rock art, they also offer several interesting applications for its analysis. Through spots clouds treatments and simulations, they permit a wide range of manipulations concerning figurations observation and study. Especially, they allow a fine perception of their volumetry. They become efficient tools for forms comparisons, very useful in the reconstruction of graphic ensemble chronologies and for inter-sites analogies. These analytical tools are illustrated by the original works done on the sculptures of Roc-aux-Sorciers (Angles-sur-l’Anglin, Vienne and Chaire-à-Calvin (Mouthiers-sur-Boëme, Charente rock-shelters.

  1. Detection of VEGF-A(xxx)b isoforms in human tissues.

    Science.gov (United States)

    Bates, David O; Mavrou, Athina; Qiu, Yan; Carter, James G; Hamdollah-Zadeh, Maryam; Barratt, Shaney; Gammons, Melissa V; Millar, Ann B; Salmon, Andrew H J; Oltean, Sebastian; Harper, Steven J

    2013-01-01

    Vascular Endothelial Growth Factor-A (VEGF-A) can be generated as multiple isoforms by alternative splicing. Two families of isoforms have been described in humans, pro-angiogenic isoforms typified by VEGF-A165a, and anti-angiogenic isoforms typified by VEGF-A165b. The practical determination of expression levels of alternative isoforms of the same gene may be complicated by experimental protocols that favour one isoform over another, and the use of specific positive and negative controls is essential for the interpretation of findings on expression of the isoforms. Here we address some of the difficulties in experimental design when investigating alternative splicing of VEGF isoforms, and discuss the use of appropriate control paradigms. We demonstrate why use of specific control experiments can prevent assumptions that VEGF-A165b is not present, when in fact it is. We reiterate, and confirm previously published experimental design protocols that demonstrate the importance of using positive controls. These include using known target sequences to show that the experimental conditions are suitable for PCR amplification of VEGF-A165b mRNA for both q-PCR and RT-PCR and to ensure that mispriming does not occur. We also provide evidence that demonstrates that detection of VEGF-A165b protein in mice needs to be tightly controlled to prevent detection of mouse IgG by a secondary antibody. We also show that human VEGF165b protein can be immunoprecipitated from cultured human cells and that immunoprecipitating VEGF-A results in protein that is detected by VEGF-A165b antibody. These findings support the conclusion that more information on the biology of VEGF-A165b isoforms is required, and confirm the importance of the experimental design in such investigations, including the use of specific positive and negative controls.

  2. Distinctive effects of rat fibroblast growth factor-2 isoforms on PC12 and Schwann cells.

    Science.gov (United States)

    Müller-Ostermeyer, F; Claus, P; Grothe, C

    2001-01-01

    Fibroblast growth factor-2 (FGF-2) is an important modulator of cell growth and differentiation and stimulates cell survival of various cells including neurons. Rat FGF-2 occurs in three isoforms, a low molecular weight 18 kD and two high molecular weight forms (21, 23 kD), representing alternative translation products from a single mRNA. The 18 kD isoform shows mainly cytoplasmatic localization, whereas the 21/23 kD FGF-2 are localized in the nucleus. In addition, the FGF-2 isoforms are differentially regulated in the sensory ganglia and peripheral nerve following nerve injury and in the adrenal medulla during post-natal development and after hormonal stimuli. The distinct intracellular distribution and differential regulation of the different FGF-2 isoforms indicate that they have unique biological roles, however, little is known about the biological effects of the high molecular weight FGF-2 isoforms. Immortalized Schwann cells and PC12 cells, which stably overexpress the different FGF-2 isoforms, showed that the different endogenous-overexpressed FGF-2 isoforms lead to dramatic modifications in cell proliferation and survival, when tested in serum-free and serum-containing medium. In contrast, application of recombinant FGF-2 isoforms on normal PC12 and immortalized Schwann cells results in similar biological effects on the proliferation and survival of the cells. Furthermore, we investigated the potential regulatory effects of endogenous-overexpressed and exogenous-applied FGF-2 isoforms on the mRNA level of the FGF-2 receptors and, additionally, on the tyrosin hydroxylase mRNA expression in PC12 cells.

  3. Non-muscle Myosin II Isoforms Co-assemble in Living Cells

    Science.gov (United States)

    Beach, Jordan R.; Shao, Lin; Remmert, Kirsten; Li, Dong; Betzig, Eric; Hammer, John A.

    2014-01-01

    SUMMARY Non-muscle myosin II (NM II) powers myriad developmental and cellular processes, including embryogenesis, cell migration, and cytokinesis [1]. To exert its functions, monomers of NM II assemble into bipolar filaments that produce a contractile force on the actin cytoskeleton. Mammalian cells express up to three isoforms of NM II (NM IIA, IIB and IIC), each of which possesses distinct biophysical properties and supports unique, as well as redundant, cellular functions [2-8]. Despite previous efforts [9-13], it remains unclear if NM II isoforms assemble in living cells to produce mixed (heterotypic) bipolar filaments, or if filaments consist entirely of a single isoform (homotypic). We addressed this question using fluorescently-tagged versions of NM IIA, IIB and IIC, isoform-specific immunostaining of the endogenous proteins, and two-color total internal reflection fluorescence structured-illumination microscopy, or TIRF-SIM, to visualize individual myosin II bipolar filaments inside cells. We show that NM II isoforms co-assemble into heterotypic filaments in a variety of settings, including various types of stress fibers, individual filaments throughout the cell, and the contractile ring. We also show that the differential distribution of NM IIA and NM IIB typically seen in confocal micrographs of well-polarized cells is reflected in the composition of individual bipolar filaments. Interestingly, this differential distribution is less pronounced in freshly-spread cells, arguing for the existence of sorting mechanism acting over time. Together, our work argues that individual NM II isoforms are potentially performing both isoform-specific and isoform-redundant functions while co-assembled with other NM II isoforms. PMID:24814144

  4. Profilin isoforms modulate astrocytic morphology and the motility of astrocytic processes.

    Directory of Open Access Journals (Sweden)

    Stefanie K Schweinhuber

    Full Text Available The morphology of astrocytic processes determines their close structural association with synapses referred to as the 'tripartite synapse'. Concerted morphological plasticity processes at tripartite synapses are supposed to shape neuronal communication. Morphological changes in astrocytes as well as the motility of astrocytic processes require remodeling of the actin cytoskeleton. Among the regulators of fast timescale actin-based motility, the actin binding protein profilin 1 has recently been shown to control the activity-dependent outgrowth of astrocytic processes. Here, we demonstrate that cultured murine astrocytes in addition to the ubiquitous profilin 1 also express the neuronal isoform profilin 2a. To analyze the cellular function of both profilins in astrocytes, we took advantage of a shRNA mediated isoform-specific downregulation. Interestingly, consistent with earlier results in neurons, we found redundant as well as isoform-specific functions of both profilins in modulating cellular physiology. The knockdown of either profilin 1 or profilin 2a led to a significant decrease in cell spreading of astrocytes. In contrast, solely the knockdown of profilin 2a resulted in a significantly reduced morphological complexity of astrocytes in both dissociated and slice culture astrocytes. Moreover, both isoforms proved to be crucial for forskolin-induced astrocytic stellation. Furthermore, forskolin treatment resulted in isoform-specific changes in the phosphorylation level of profilin 1 and profilin 2a, leading to a PKA-dependent phosphorylation of profilin 2a. In addition, transwell assays revealed an involvement of both isoforms in the motility of astrocytic processes, while FRAP analysis displayed an isoform-specific role of profilin 1 in the regulation of actin dynamics in peripheral astrocytic processes. Taken together, we suggest profilin isoforms to be important modulators of astrocytic morphology and motility with overlapping as well as

  5. Learning-dependent gene expression of CREB1 isoforms in the molluscan brain

    Directory of Open Access Journals (Sweden)

    Hisayo Sadamoto

    2010-05-01

    Full Text Available Cyclic AMP-responsive element binding protein1 (CREB1 has multiple functions in gene regulation. Various studies have reported that CREB1-dependent gene induction is necessary for memory formation and long-lasting behavioral changes in both vertebrates and invertebrates. In the present study, we characterized Lymnaea CREB1 (LymCREB1 mRNA isoforms of spliced variants in the central nervous system (CNS of the pond snail Lymnaea stagnalis. Among these spliced variants, the three isoforms that code a whole LymCREB1 protein are considered to be the activators for gene regulation. The other four isoforms, which code truncated LymCREB1 proteins with no kinase inducible domain, are the repressors. For a better understanding of the possible roles of different LymCREB1 isoforms, the expression level of these isoform mRNAs was investigated by a real-time quantitative RT-PCR method. Further, we examined the changes in gene expression for all the isoforms in the CNS after conditioned taste aversion (CTA learning or backward conditioning as a control. The results showed that CTA learning increased LymCREB1 gene expression, but it did not change the activator/repressor ratio. Our findings showed that the repressor isoforms, as well as the activator ones, are expressed in large amounts in the CNS, and the gene expression of CREB1 isoforms appeared to be specific for the given stimulus. This was the first quantitative analysis of the expression patterns of CREB1 isoforms at the mRNA level and their association with learning behavior.

  6. The polysaccharide inulin is characterized by an extensive series of periodic isoforms with varying biological actions.

    Science.gov (United States)

    Cooper, Peter D; Barclay, Thomas G; Ginic-Markovic, Milena; Petrovsky, Nikolai

    2013-10-01

    In studying the molecular basis for the potent immune activity of previously described gamma and delta inulin particles and to assist in production of inulin adjuvants under Good Manufacturing Practice, we identified five new inulin isoforms, bringing the total to seven plus the amorphous form. These isoforms comprise the step-wise inulin developmental series amorphous → alpha-1 (AI-1) → alpha-2 (AI-2) → gamma (GI) → delta (DI) → zeta (ZI) → epsilon (EI) → omega (OI) in which each higher isoform can be made either by precipitating dissolved inulin or by direct conversion from its precursor, both cases using regularly increasing temperatures. At higher temperatures, the shorter inulin polymer chains are released from the particle and so the key difference between isoforms is that each higher isoform comprises longer polymer chains than its precursor. An increasing trend of degree of polymerization is confirmed by end-group analysis using (1)H nuclear magnetic resonance spectroscopy. Inulin isoforms were characterized by the critical temperatures of abrupt phase-shifts (solubilizations or precipitations) in water suspensions. Such (aqueous) "melting" or "freezing" points are diagnostic and occur in strikingly periodic steps reflecting quantal increases in noncovalent bonding strength and increments in average polymer lengths. The (dry) melting points as measured by modulated differential scanning calorimetry similarly increase in regular steps. We conclude that the isoforms differ in repeated increments of a precisely repeating structural element. Each isoform has a different spectrum of biological activities and we show the higher inulin isoforms to be more potent alternative complement pathway activators.

  7. Mécanique des fluides fondamentale

    CERN Document Server

    Zeytounian, Radyadour K

    1991-01-01

    Ce cours de mecanique des fluides est avant tout un cours theorique qui repond aux questions fondamentales de ce sujet de recherche. Les quatre premiers chapitres presentent les equations propres a determiner l'ecoulement de fluide considere et diverses solutions. Les chapitres 5 et 6sont consacres aux problemes lies la stabilite, aux bifurcations et aux comportements chaotiques. Le livre donne un vision globale des questions traitees en mecanique des fluides qui sont a la base de toute la recherche, de la modelisation et de toutes les applications dans ce domaine.

  8. Écriture scientifique et évolution des croyances, des connaissances ou des conceptions des enseignants en reprise d’étude

    Directory of Open Access Journals (Sweden)

    Delarue-Breton Catherine

    2016-01-01

    Full Text Available Si l’on s’accorde assez unanimement – c’est à dire aux plans simultanément scientifique et institutionnel – à reconnaitre que l’initiation à la recherche est devenue nécessaire dans la formation des enseignants, les modalités de cette initiation, ses visées et ses effets font cependant peu l’objet, encore, d’un consensus, et dans certains cas, divisent. Nous avons pu étudier à diverses reprises, à partir de différentes versions des mémoires, le rôle de l’écriture scientifique dans l’appropriation des concepts théoriques par les étudiants de master MEEF (Métiers de l’Enseignement, de l’Éducation, de la Formation en formation initiale, autrement dit par les étudiants issus de licence, se destinant au métier de professeur des écoles. Nous avons ainsi pu mettre au jour d’une part la diversité de ces modes d’appropriation conceptuelle, et, en corrélation avec celle-ci, la diversité des modes d’analyse des situations scolaires, et d’autre part des formes d’évolution ou de stagnation des croyances, connaissances, conceptions de ces futurs enseignants sur le métier, sur les élèves, sur les disciplines, sur l’apprentissage. Notre propos est ici de nous poser des questions comparables, concernant le rapport entre appropriation théorique et évolution des conceptions non pas d’enseignants en formation initiale, mais d’enseignants titulaires en reprise d’étude, parfois eux-mêmes formateurs, inscrits en master MEEF avec validation des acquis. Nous questionnons ainsi le rôle de la recherche et de l’écriture scientifique dans la formation continue des enseignants.

  9. MESURE DE LA PERFORMANCE GLOBALE DES ENTREPRISES

    OpenAIRE

    Renaud, Angèle; Berland, Nicolas

    2007-01-01

    International audience; L'engagement des entreprises dans le développement durable consiste à conjuguer performance et responsabilité. La performance financière ne suffit plus à apprécier la performance d'une entreprise. Dès lors, les entreprises doivent mesurer leurs progrès à partir d'une performance plus globale incluant, en dehors de la dimension économique, des dimensions sociale et environnementale. A présent, comment mesurer cette performance globale ? Existe-il des outils de mesure gl...

  10. A l'écoute des professionnels

    CERN Document Server

    Lebre-Peytard, Monique

    1994-01-01

    Cet ouvrage s'adresse aux étudiants de niveau moyen ou avancé en français. Il propose un travail d'écoute, de réflexion et d'expression autour d'interview authentiques. Des professionnels parlent de leur métier, des entreprises où ils travaillent, des fonctions qu'ils occupent... Ils appartiennent à divers secteurs de l'économie : banque, mode, tourisme, agriculture, télécommunications, etc.

  11. Comprehensive identification of cytochrome p450 isoforms from solubility-based fractionated rat liver microsomes.

    Science.gov (United States)

    Yamanaka, Hidenori; Takeyoshi, Masahiro; Minobe, Yasushi; Yakabe, Yoshikuni; Takatsuki, Mineo; Sato, Hisaya

    2007-12-01

    Cytochrome P450 isoforms from male rat liver microsomes were comprehensively identified using nano liquid chromatography tandem mass spectrometry (nanoLC-MS/MS). The enrichment of P450, an endomembrane-anchored heme protein, was achieved by solubility-based protein fractionation, and greatly improved the total number of identified P450 isoforms. LC-MS/MS analysis of fractions resulted in the identification of total 36 P450 isoforms. The combination of proteomic analysis and the solubility-based fractionation would provide powerful tool for the expression analysis of the superfamily proteins having great similarities between the amino acids sequences.

  12. Purification and Characterization of Two Voltage-Dependent Anion Channel Isoforms from Plant Seeds1

    Science.gov (United States)

    Abrecht, Helge; Wattiez, Ruddy; Ruysschaert, Jean-Marie; Homblé, Fabrice

    2000-01-01

    Mitochondria were isolated from imbibed seeds of lentil (Lens culinaris) and Phaseolus vulgaris. We copurified two voltage-dependent anion channel from detergent solubilized mitochondria in a single purification step using hydroxyapatite. The two isoforms from P. vulgaris were separated by chromatofocusing chromatography in 4 m urea without any loss of channel activity. Channel activity of each isoform was characterized upon reconstitution into diphytanoyl phosphatidylcholine planar lipid bilayers. Both isoforms form large conductance channels that are slightly anion selective and display cation selective substates. PMID:11080295

  13. Intensidad exportadora e interacción entre fortalezas del marketing mix: un análisis basado en redes neuronales artificiales || Export Intensity and Interaction between Marketing Mix: An Analysis based on Artificial Neural Networks

    Directory of Open Access Journals (Sweden)

    Gutiérrez-Villar, Belén

    2014-12-01

    Full Text Available Entre los factores determinantes de la actuación exportadora de las empresas, numerosos estudios han subrayado la relevancia del mix de marketing. Generalmente, la mayoría de los estudios abordan el análisis de las variables centrado en estrategias específicas, en particular fenómenos de estandarización-adaptación. El presente estudio analiza si existe un efecto interactivo de fortalezas generadas en las diferentes variables del mix de marketing que pueda asociarse a diferentes perfiles exportadores. Para ello, se ha empleado el algoritmo Extreme Learning Machine (ELM dentro de los procedimientos Perceptrón Multicapa (MLP de Redes Neuronales Artificiales (RNA. Asimismo, el análisis se combina con un novedoso procedimiento de análisis de sensibilidad desarrollado ad hoc para este estudio, el cual permite conocer los efectos individuales e interactivos de las variables predictoras sobre la variable dependiente en problemas clasificatorios de naturaleza dicotómica. Los resultados obtenidos nos permiten corroborar la existencia de los efectos interactivos postulados, poniendo al mismo tiempo de manifiesto la utilidad de las RNA y del análisis de sensibilidad propuesto para la investigación en el área de marketing y, específicamente, para los estudios de internacionalización de empresas. || Among the determining factors in export activity, many studies have highlighted the relevance of the marketing mix. Generally, the majority of them use a variables analysis to focus on specific strategies, in particular, standardized-adaptations. This paper analyzes if there is an interactive effect of strength generated in different variables of the marketing mix that can be associated with different export profiles. The Extreme Learning Machine (ELM algorithm has been used within the Multilayer Perceptron (MLP of Artificial Neural Networks (ANN. In addition, the analyses combine a novel approach for sensitivity analysis developed ad hoc for this

  14. Utilización de redes neuronales para la determinación de respuestas de sitio a partir de Ondas coda Aplicación para Armenia Colombia

    Directory of Open Access Journals (Sweden)

    Vargas Jiménez Carlos A.

    2001-08-01

    neuronales facilitan el proceso de deducción de funciones de transferencia lineales y no lineales orientadas a la evaluación dinámica de suelos.

  15. Differential susceptibility of RAE-1 isoforms to mouse cytomegalovirus.

    Science.gov (United States)

    Arapovic, Jurica; Lenac, Tihana; Antulov, Ronald; Polic, Bojan; Ruzsics, Zsolt; Carayannopoulos, Leonidas N; Koszinowski, Ulrich H; Krmpotic, Astrid; Jonjic, Stipan

    2009-08-01

    The NKG2D receptor is one of the most potent activating natural killer cell receptors involved in antiviral responses. The mouse NKG2D ligands MULT-1, RAE-1, and H60 are regulated by murine cytomegalovirus (MCMV) proteins m145, m152, and m155, respectively. In addition, the m138 protein interferes with the expression of both MULT-1 and H60. We show here that one of five RAE-1 isoforms, RAE-1delta, is resistant to downregulation by MCMV and that this escape has functional importance in vivo. Although m152 retained newly synthesized RAE-1delta and RAE-1gamma in the endoplasmic reticulum, no viral regulator was able to affect the mature RAE-1delta form which remains expressed on the surfaces of infected cells. This differential susceptibility to downregulation by MCMV is not a consequence of faster maturation of RAE-1delta compared to RAE-1gamma but rather an intrinsic property of the mature surface-resident protein. This difference can be attributed to the absence of a PLWY motif from RAE-1delta. Altogether, these findings provide evidence for a novel mechanism of host escape from viral immunoevasion of NKG2D-dependent control.

  16. Contrôle des Circuits Auxiliaires des PFW (arrêt machines 2002/2003)

    CERN Document Server

    Ottaviani, J

    2003-01-01

    Les PFW sont des nappes polaires installées sur les pôles des aimants du PS. Elles sont au nombre de 4 par unité d aimant et constituées d un circuit principal (dans lequel circule le courant désiré selon le cycle magnétique utilisé) et de circuits auxiliaires. Les circuits auxiliaires sont des enroulements de correction (boucles de tour et pick-up brasés sur les enroulements). Pendant la variation du champ magnétique de l aimant PS, on utilise des tensions induites dans ces circuits auxiliaires pour corriger les erreurs de champ dues aux courants de Foucault dans la chambre à vide. Chaque année, pendant l arrêt machine, on vérifie si les caractéristiques des circuits auxiliaires correspondent aux normes (mesures des résistances des boucles de tours, résistances entre pick-up et isolation des circuits) afin de faire un suivi. Les 404 PFW sont ainsi vérifiées. Dans cette note, on ne relèvera que les PFW ayant des défauts (valeurs hors tolérances, boucles ouvertes ou en court-circuit, déf...

  17. Exploitation des connaissances issues des processus de retour d'expérience industriels

    OpenAIRE

    Jabrouni, Hicham

    2012-01-01

    Depuis plusieurs années, dans le secteur industriel, l’amélioration continue constitue un aspect important de la famille de normes ISO 9000 maintenue par l’organisation ISO (International Organization for Standardization). Elle se concentre sur l’amélioration de la satisfaction du client en passant par des améliorations continues et incrémentales des produits, des services et des processus. Afin de répondre à ces exigences, un point clé consiste à optimiser le processus de résolution de probl...

  18. Des changements concevables pour le secteur des transports dans l'objectif "Facteur 4"

    OpenAIRE

    Raux, Charles

    2007-01-01

    Au niveau mondial, les transports consomment 20% de l'énergie, dont 80% dans les transports routiers et 10% dans les transports aériens, et 95% de cette énergie est à base de pétrole. Le secteur des transports est l'un des principaux émetteurs de gaz à effet de serre dans la plupart des pays, et notamment du CO2 issu de la combustion des carburants fossiles. Pour que les transports contribuent significativement à l'objectif du « Facteur 4 » en 2050, trois pistes différentes mais complémentair...

  19. Typologie des investisseurs institutionnels et performance des firmes dans le contexte français

    OpenAIRE

    Jean-Michel SAHUT; Othmani Gharbi, Hidaya

    2011-01-01

    L’objet de cet article est d’analyser la relation entre les investisseurs institutionnels et la performance financière des firmes. Dans ce contexte, nous proposons une nouvelle typologie fondée sur la variété des comportements des institutionnels (passifs ou engagés (actifs)) vis-à-vis de la gestion et du gouvernement des firmes qu’ils détiennent en portefeuille, et identifions les principaux facteurs pouvant influencer ces comportements. Nos résultats corroborent, en général, un impact posit...

  20. Etude structurale des sites de nodulation et induction des nodules caulinaires chez Sesbania pubescens

    OpenAIRE

    TOMEKPE, Kodjo; Traore, A S; Ndiaye, S.; Spencer-Barreto, M.M.; Détrez, C.

    1992-01-01

    #Sesbania pubescens$ est une légumineuse annuelle voisine de #Sesbania rostrata$ qui se rencontre généralement sur les sols temporairement inondés de l'Afrique de l'Ouest. Des sites de nodulation préformes ont été mis en évidence sur sa tige et ses branches. Ces sites prennent naissance à l'aisselle des cotylédons et des feuilles. Comme chez la plupart des légumineuses à nodules de tige, les sites de nodulation caulinaire de #S. pubescens$ se développent en racines lorsque les tiges sont imme...

  1. Molekularbiologische Untersuchungen des Leitgewebes und der Stressphysiologie des Breitwegerichs (Plantago major L.)

    OpenAIRE

    Pommerrenig, Benjamin

    2008-01-01

    Die Leitbündel Höherer Pflanzen stellen die wesentlichen Gewebe zur Verteilung von Assimilaten, zum Transport von Wasser und zur Übermittlung von Informationen innerhalb und zwischen den einzelnen pflanzlichen Organen dar. Die Zellen des vaskulären Gewebes unterscheiden sich von denen des Mesophylls nicht nur in ihrer Gestalt und Funktion, sondern vor allem in ihrer Genexpression. In dieser Arbeit wurde zur Untersuchung des vaskulären Transkriptoms des Breitwege-richs (Plantago major) eine cD...

  2. Les lexiques des jeunes dans les discours écrits des blogs : pour une approche descriptive

    Directory of Open Access Journals (Sweden)

    Lorenz Paulina

    2014-07-01

    Full Text Available S’il ne faut pas confondre ce qui est appelé communément « langage SMS » et « langage des jeunes », nous avancerons toutefois que les pratiques langagières écrites des jeunes sur les supports modernes de communication, et notamment dans les blogs, présentent des particularités encore peu étudiées. Les traits observables les plus saillants sont ceux de la morphologie graphique, mais il n’en reste pas moins que certains jeunes utilisent des lexiques particuliers dans les blogs qui méritent une attention particulière. Nous nous proposons donc d’étudier les différents lexiques utilisés par des jeunes de 12 à 22 ans dans les blogs de la plateforme skyrock.com aux niveaux de sa diversité, de ses usages sémantiques variable d’un scripteur à l’autre pour un même mot, et de ses particularités morphosyntaxiques. En effet, nous observerons des lexiques divers allant des lexiques perçus comme relevant des cités à des lexiques perçus comme étant « branchés-jeunes », ou encore à des lexiques propres aux supports modernes de communication. Ainsi, nous analyserons des lexiques variés dans leurs usages écrits tels que bolos, bédave, gérer, ouf, tigen, ou encore des lexiques empruntés tels que mouv, full, etc., ainsi que des emplois particuliers de séquences autonomes comme lol. Quelques questions périphériques complèteront notre analyse telle que celles qui concerne l’influence des usages lexicaux entre pairs et le savoir partagé, la distinction de différentes communautés en fonction des lexiques utilisés, ou encore la variabilité des représentations graphiques d’un même mot.

  3. Peste des Petits Ruminants Virus.

    Science.gov (United States)

    Baron, M D; Diallo, A; Lancelot, R; Libeau, G

    2016-01-01

    Peste des petits ruminants virus (PPRV) causes a severe contagious disease of sheep and goats and has spread extensively through the developing world. Because of its disproportionately large impact on the livelihoods of low-income livestock keepers, and the availability of effective vaccines and good diagnostics, the virus is being targeted for global control and eventual eradication. In this review we examine the origin of the virus and its current distribution, and the factors that have led international organizations to conclude that it is eradicable. We also review recent progress in the molecular and cellular biology of the virus and consider areas where further research is required to support the efforts being made by national, regional, and international bodies to tackle this growing threat. © 2016 Elsevier Inc. All rights reserved.

  4. Utilisation des mousses et des pâtes syntactiques pour combler les lacunes des poteries archéologiques

    Directory of Open Access Journals (Sweden)

    Viviane Bechoux

    2008-10-01

    Full Text Available Filling potteries missing parts with plaster raises now complex problems during the de-restoration treatments. Ceramics restorers have recurrently questions on the use of plaster in the field of archaeological ceramics restoration. Some researches point to the choice of other filling materials. At the present time other methods can be devised by proposing plaster replacement by a lightened composite material: syntactic foams and pastes.Le comblement des lacunes des poteries à partir du plâtre pose actuellement des problèmes complexes lors des traitements de dérestauration. Les restaurateurs de céramiques s'interrogent de manière récurrente sur l'utilisation du plâtre dans le domaine de la restauration des céramiques archéologiques. Des recherches s'orientent vers le choix d'autres matériaux d'obturation. Actuellement, d'autres méthodes peuvent être mises en place en proposant le remplacement du plâtre par un matériau composite allégé, les mousses et les pâtes syntactiques.

  5. Effect of ovine luteinizing hormone (oLH) charge isoforms on VEGF and cAMP production.

    Science.gov (United States)

    Montero-Pardo, Arnulfo; Diaz, Daniel; Olivares, Aleida; González-Padilla, Everardo; Murcia, Clara; Gómez-Chavarín, Margarita; Gutiérrez-Ospina, Gabriel; Perera-Marín, Gerardo

    2015-12-01

    Although an increase in VEGF expression and synthesis in association with LH has been established; it is unknown if all LH isoforms act similarly. This study evaluated the production of cAMP and VEGF among LH isoforms in two in vitro bioassays. The LH was obtained from hypophyses and the group of isoforms was isolated by chromatofocusing. cAMP production was assessed using the in vitro bioassay of HEK-293 cells and VEGF production was evaluated in granulosa cells. Immunological activity was measured with a homologous RIA. Immunoactivity and bioactivity for each isoform were compared against a standard, by estimating the IC50 and the EC50. The basic isoforms were more immunoactive than the standard. The neutral and the moderately acidic had an immunological activity similar to the standard. The acidic isoform was the least immunoreactive. cAMP production at the EC50 dose was similar among the basic isoforms, the moderately acidic and the standard; for the neutral and the acidic, the EC50 dose was higher. It was observed that compared with the control, VEGF production at the lowest LH dose was no different in the standard and each isoform. In the intermediate dose, a positive response was caused in the standard and the neutral and basic isoforms. Although the acidic isoform showed a dose-dependent response, it was not significant relative to the control. In conclusion, the basic isoform generated the greatest cAMP and VEGF production, similar to the reference standard, and the acidic the smallest.

  6. Des Balkans About the Balkans

    Directory of Open Access Journals (Sweden)

    Predrag Matvejević

    2012-05-01

    Full Text Available Les Balkans témoignent de contradictions géographiques et politiques, perceptibles dans le changement d’appellation des lieux au cours de l’Histoire : de la péninsule illyrienne, grecque, byzantine à la « Turquie d’Europe » plus récente. Il est resté une dénomination venue des Turcs, les « Balkans ». Cette presqu’île n’offre pas de frontières naturelles difficiles à franchir. Les délimitations des géographes et historiens sont relatives et souvent arbitraires, et donc contestées. Sa définition est difficile, car, étant associée à la progression des troupes ottomanes, cette « zone de turbulences » garde aux yeux de certains un caractère négatif. Marqués par les bouleversements telluriques et historiques, les Balkans témoignent d’une pluralité et d’une variété démographiques, ce qui pose un grand nombre de questions en termes de rapports ethniques, de constructions identitaires et territoriales. Les revendications s’appuient aussi bien sur le mythe que sur l’Histoire. L’apparition de l’État-nation a constitué dans ces régions de multiples fractures à l’image du schisme chrétien de 1054, divisant Églises et croyances, empires et pouvoirs, styles et écritures. L’islam a engendré de nouvelles oppositions ou intolérances. Les puissances étrangères sont intervenues dans ce jeu pour tenter de le stabiliser tout en déterminant leurs sphères d’influence, figeant par le jeu des traités une multitude de questions irrésolues ou conflictuelles, sans apporter de réponse durable. Cet inachevé, dans une histoire faite de partages, suscite des frustrations, et nécessite une réécriture qui tarde à se faire jour, faute d’apaisement entre les différentes sensibilités. La littérature se fait l’écho de ces situations par l’évocation de la cruauté, sous l’occupation ottomane (cf. Ivo Andrić notamment, mais aussi dans l’actualité récente. « Cet espace qui produit

  7. Detection of isoform-specific fibroblast growth factor receptors by whole-mount in situ hybridization in early chick embryos.

    Science.gov (United States)

    Nishita, Junko; Ohta, Sho; Bleyl, Steven B; Schoenwolf, Gary C

    2011-06-01

    We have developed "b" and "c" isoform-specific chicken fibroblast growth factor (FGF) receptor 1-3 probes for in situ hybridization. We rigorously demonstrate the specificity of these probes by using both dot blot hybridization and whole-mount in situ hybridization during neurulation and early postneurulation stages, and we compare expression patterns of each of the three isoform-specific probes to one another and to generic probes to each of the three (non-isoform-specific) FGF receptors. We show that the expression pattern of each receptor is represented by the collective expression of each of its two isoforms, with the expression of each FGF receptor being most similar to that of its "c" isoform at two of the three stages studied, and that tissue and stage differences exist in the patterns of expression of the six isoforms. We demonstrate the usefulness of these probes for defining the differential tissue expression of FGF receptor 1-3 isoforms.

  8. La reputation scientifique contestee des freres Bogdanov

    CERN Multimedia

    Morin, H

    2002-01-01

    "Les celebres jumeaux sont revenus a la television apres avoir obtenu des theses en mathematiques et physique theorique. Depuis, ils sont la cible de virulentes critiques portant sur la qualite reelle de leurs travaux" (1 page).

  9. The enhancement of existing DES Maplet interface

    Science.gov (United States)

    Abdullah, Nur Lina; Mutalip, Rasidah Abdull; Abdullah, Kamilah

    2014-07-01

    This study pertains to the process of Data Encryption Standard, DES. DES consists of encryption and decryption processes linked with mathematical elements such as algebra and number theory. Preliminary, studies revealed that most of mathematics students face a problem in understanding the complicated process of DES. In modern learning methods, learning environment becomes more interesting with the use of computer and a variety of mathematical software packages. Several mathematical softwares such as Maple, Mathematica, Mathlab and Sage were developed in order to fulfill the specific calculation requirements. Correspondingly, motivated from that, this study incorporated with Maple to enhance the existing DES Maplet interface to be more interactive and user-friendly compared to the original version.

  10. GROSSESSE GEMELLA IRE ET EVOLUTION DES JUMEAUX

    OpenAIRE

    SALAH, HANANE; ALLAOUI, AMINA

    2012-01-01

    Cette étude prospective succincte nous a permis de réunir les informations suivantes - La grande fréquence des grossesses gémellaires dans le mois de septembre. - L'âge jeune des mamans. - La plupart des jumeaux occupent la première ou la deuxième place dans la fratrie. - Le bon état de santé des mamans avant la grossesse par l'absence d'antécédents pathologiques chez elles. - Un très faible tôt d'antécédent personnel de grossesse gémellaire antérieure contre une...

  11. Pyrotechnie : une évaluation des risques

    OpenAIRE

    Aufauvre, Lionel; Marlair, Guy; Michot, Christian

    2006-01-01

    Depuis une dizaine d'années, la pyrotechnie tend à se démocratiser, comme en témoigne le développement du marché des artifices de divertissement pour le grand public en France. Elle touche de même l'ensemble de la population dans des applications comme les équipements de sécurité des véhicules automobiles de type airbags ou prétensionneurs de ceinture de sécurité. En effet, ces équipements mettent en oeuvre des matériaux énergétiques adaptés au temps de réaction, extrêmement court, nécessaire...

  12. Wood duck studies : Des Lacs NWR

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This memorandum summarizes wood duck studies on Des Lacs National Wildlife Refuge and provides a brief history on the status of wood ducks on the edge of their...

  13. Table des abréviations

    OpenAIRE

    2015-01-01

    A Textes juridiques al. : alinéa art. : article A.u.-Com. : Acte uniforme portant droit commercial général A.u.-Compt. : Acte uniforme portant harmonisation des comptabilités des entreprises A.u.-Proc.coll. : Acte uniforme portant organisation des procédures collectives d’apurement du passif A.u.-Soc. : Acte uniforme relatif aux sociétés commerciales et au groupement d’intérêt économique C.ass. : Code des assurances C.civ. : Code civil C.com. : Code de commerce C.E.S.D.H. : Convention europée...

  14. VAMP/synaptobrevin isoforms 1 and 2 are widely and differentially expressed in nonneuronal tissues

    Science.gov (United States)

    1996-01-01

    VAMP/synaptobrevin is part of the synaptic vesicle docking and fusion complex and plays a central role in neuroexocytosis. Two VAMP (vesicle- associated membrane protein) isoforms are expressed in the nervous system and are differently distributed among the specialized parts of the tissue. Here, VAMP-1 and -2 are shown to be present in all rat tissues tested, including kidney, adrenal gland, liver, pancreas, thyroid, heart, and smooth muscle. The two isoforms are differentially expressed in various tissues and their level may depend on differentiation. VAMP-1 is restricted to exocrine pancreas and to kidney tubular cells, whereas VAMP-2 is the predominant isoform present in Langerhans islets and in glomerular cells. Both isoforms show a patchy vesicular intracellular distribution in confocal microscopy. The present results provide evidence for the importance of neuronal VAMP proteins in the physiology of all cells. PMID:8567721

  15. [Mechanism of malate dehydrogenase isoform formation in Sphaerotilus natans D-507 under different cultivation conditions].

    Science.gov (United States)

    Eprintsev, A T; Falaleeva, M I; Arabtseva, M A; Lavrinenko, I A; Parfenova, I V; Grechkina, M V; Abud, F S

    2011-01-01

    Electrophoretically homogenous preparations of malate dehydrogenase (MDH) isoforms of the bacteria Sphaerotilus natans D-507 with specific activity 7.46 U/mg and 5.74 U/mg with respect to protein concentration have been obtained. The dimeric isoform of the enzyme was shown to function under organotrophic growth conditions, whereas the tetrameric isoform was induced under mixotrophic cultivation conditions. PCR-analysis revealed a single gene encoding the malate dehydrogenase molecule. The topography of the MDH isoform surface was studied by atomic-force microscopy, and a 3D-structure of the enzyme was obtained. Spectraphotometric analysis data allowed us to suggest that stabilization of the tetrameric form of MDH is due to additional bounds implicated in the quaternary structure formation.

  16. Sensitivity of small myosin II ensembles from different isoforms to mechanical load and ATP concentration

    Science.gov (United States)

    Erdmann, Thorsten; Bartelheimer, Kathrin; Schwarz, Ulrich S.

    2016-11-01

    Based on a detailed crossbridge model for individual myosin II motors, we systematically study the influence of mechanical load and adenosine triphosphate (ATP) concentration on small myosin II ensembles made from different isoforms. For skeletal and smooth muscle myosin II, which are often used in actomyosin gels that reconstitute cell contractility, fast forward movement is restricted to a small region of phase space with low mechanical load and high ATP concentration, which is also characterized by frequent ensemble detachment. At high load, these ensembles are stalled or move backwards, but forward motion can be restored by decreasing ATP concentration. In contrast, small ensembles of nonmuscle myosin II isoforms, which are found in the cytoskeleton of nonmuscle cells, are hardly affected by ATP concentration due to the slow kinetics of the bound states. For all isoforms, the thermodynamic efficiency of ensemble movement increases with decreasing ATP concentration, but this effect is weaker for the nonmuscle myosin II isoforms.

  17. Neuronal and intestinal protein kinase d isoforms mediate Na+ (salt taste)-induced learning.

    Science.gov (United States)

    Fu, Ya; Ren, Min; Feng, Hui; Chen, Lu; Altun, Zeynep F; Rubin, Charles S

    2009-08-11

    Ubiquitously expressed protein kinase D (PKD) isoforms are poised to disseminate signals carried by diacylglycerol (DAG). However, the in vivo regulation and functions of PKDs are poorly understood. We show that the Caenorhabditis elegans gene, dkf-2, encodes not just DKF-2A, but also a second previously unknown isoform, DKF-2B. Whereas DKF-2A is present mainly in intestine, we show that DKF-2B is found in neurons. Characterization of dkf-2 null mutants and transgenic animals expressing DKF-2B, DKF-2A, or both isoforms revealed that PKDs couple DAG signals to regulation of sodium ion (Na+)-induced learning. EGL-8 (a phospholipase Cbeta4 homolog) and TPA-1 (a protein kinase Cdelta homolog) are upstream regulators of DKF-2 isoforms in vivo. Thus, pathways containing EGL-8-TPA-1-DKF-2 enable learning and behavioral plasticity by receiving, transmitting, and cooperatively integrating environmental signals targeted to both neurons and intestine.

  18. Glial fibrillary acidic protein isoform expression in plaque related astrogliosis in Alzheimer's disease

    NARCIS (Netherlands)

    Kamphuis, W.; Middeldorp, Jinte; Kooijman, Lieneke; Sluijs, Jacqueline A; Kooi, Evert-Jan; Moeton, Martina; Freriks, Michel; Mizee, Mark R; Hol, Elly M

    2014-01-01

    In Alzheimer's disease (AD), amyloid plaques are surrounded by reactive astrocytes with an increased expression of intermediate filaments including glial fibrillary acidic protein (GFAP). Different GFAP isoforms have been identified that are differentially expressed by specific subpopulations of ast

  19. Receptor-isoform-selective insulin analogues give tissue-preferential effects

    DEFF Research Database (Denmark)

    Vienberg, Sara Gry; Bouman, Stephan D; Sørensen, Heidi

    2011-01-01

    The relative expression patterns of the two IR (insulin receptor) isoforms, +/- exon 11 (IR-B/IR-A respectively), are tissue-dependent. Therefore we have developed insulin analogues with different binding affinities for the two isoforms to test whether tissue-preferential biological effects can...... be attained. In rats and mice, IR-B is the most prominent isoform in the liver (> 95%) and fat (> 90%), whereas in muscles IR-A is the dominant isoform (> 95%). As a consequence, the insulin analogue INS-A, which has a higher relative affinity for human IR-A, had a higher relative potency [compared with HI...... (human insulin)] for glycogen synthesis in rat muscle strips (26%) than for glycogen accumulation in rat hepatocytes (5%) and for lipogenesis in rat adipocytes (4%). In contrast, the INS-B analogue, which has an increased affinity for human IR-B, had higher relative potencies (compared with HI...

  20. Altered β-Amyloid Precursor Protein Isoforms in Mexican Alzheimer’s Disease Patients

    Directory of Open Access Journals (Sweden)

    V. J. Sánchez-González

    2006-01-01

    Full Text Available Objective: To determine the β-amyloid precursor protein (βAPP isoforms ratio as a risk factor for Alzheimer’s Disease and to assess its relationship with demographic and genetic variables of the disease.

  1. L 'Analyse des Organisations en Afrique:

    African Journals Online (AJOL)

    croissance économique, développement des techniques industrielles, nécessité d'une plus ... légale se caractérise par la consécration des règles de droit et d'un certain nombre ..... mais un système ouvert qui entretient avec son environnement une relation .... C'est l'affaire de tous, que l'on soit supérieur ou subordonné.

  2. Tabanidae (Diptera) des pays-bas

    NARCIS (Netherlands)

    Leclercq, M.

    1967-01-01

    INTRODUCTION Cette mise au point des Tabanides des Pays-Bas a été rendue possible grâce à la collaboration de M. V. S. van der Goot, département d'Entomologie, Zoölogisch Museum, Amsterdam, que nous remercions pour son amabilité. Nous avons pu étudier aussi les collections du Rijksmuseum van Natuurl

  3. Probleme des elektiven Mutismus bei Jugendlichen

    OpenAIRE

    1983-01-01

    Nach einem Exkurs zur Terminologie des Begriffs elektiver Mutismus und zur Symptomatologie dieser Erkrankung wird anhand einer Krankengeschichte die Hauptproblematik der Psychotherapie mit einem jugendlichen Mutisten erörtert. Besondere Erwähnung finden dabei 1. der äußerst schwierige Abschnitt von der Kontaktaufnahme bis zur "regelrechten" Gesprächspsychotherapie und 2. der Konflikt, der in diesem speziellen Fall der Entstehung des Mutismus zugrunde liegt. Hinweise zur Arbeit mit de...

  4. Der Machtmensch: zur Dramatologie des Politikers

    OpenAIRE

    Hitzler, Ronald

    1991-01-01

    Der Beitrag erörtert das Anforderungsprofil des modernen Politikers, wobei unter anderem folgende Aspekte beleuchtet werden: (1) Die Darstellungskompetenz, (2) Machiavellismus, (3) die typische Persönlichkeit des Politikers (Harold D. Laswell), (4) Politik als Beruf (M. Weber), (5) die wichtigsten Bedingungen für eine erfolgreiche Politikerkarriere, (6) politisches Handeln verstanden als Inszenierungsarbeit, (7) Politik als Ritual (Murray Edelman), (8) die Dramatologie der Politik sowie (9) d...

  5. Analyse de la mobilité des paysages et des systèmes sociaux en Thessalie : l’apport des images Landsat TM

    Directory of Open Access Journals (Sweden)

    Bernard Helly

    2000-05-01

    Full Text Available Une équipe pluridisciplinaire tente d’identifier la répartition des sites observables ou enfouis dans la plaine de Larissa. L’analyse d’image Landsat TM a permis d’identifier l’extension réelle des épandages, des systèmes de failles mais aussi des trames anthropiques qu’aucun autre document n’illustre.

  6. Enhanced expression of two discrete isoforms of matrix metalloproteinase-2 in experimental and human diabetic nephropathy

    Science.gov (United States)

    Bae, Sun Sik; Lee, Min Young; Rhee, Harin; Kim, Il Young; Seong, Eun Young; Lee, Dong Won; Lee, Soo Bong; Kwak, Ihm Soo; Lovett, David H.

    2017-01-01

    Background We recently reported on the enhanced expression of two isoforms of matrix metalloproteinase-2 (MMP-2) in human renal transplantation delayed graft function. These consist of the conventional secreted, full length MMP-2 isoform (FL-MMP-2) and a novel intracellular N-Terminal Truncated isoform (NTT-MMP-2) generated by oxidative stress-mediated activation of an alternate promoter in the MMP-2 first intron. Here we evaluated the effect of hyperglycemia and diabetes mellitus on the in vitro and in vivo expression of the two MMP-2 isoforms. Methods We quantified the abundance of the FL-MMP-2 and NTT-MMP-2 transcripts by qPCR in HK2 cells cultured in high glucose or 4-hydroxy-2-hexenal (HHE) and tested the effects of the NF-κB inhibitor pyrrolidine dithiocarbamate (PDTC). The streptozotocin (STZ) murine model of Type I diabetes mellitus and renal biopsies of human diabetic nephropathy were used in this study. Results Both isoforms of MMP-2 in HK2 cells were upregulated by culture in high glucose or with HHE. PDTC treatment did not suppress high glucose-mediated FL-MMP-2 expression but potently inhibited NTT-MMP-2 expression. With STZ-treated mice, renal cortical expression of both isoforms was increased (FL-MMP-2, 1.8-fold; NTT-MMP-2, greater than 7-fold). Isoform-specific immunohistochemical staining revealed low, but detectable levels of the FL-MMP-2 isoform in controls, while NTT-MMP-2 was not detected. While there was a modest increase in tubular epithelial cell staining for FL-MMP-2 in STZ-treated mice, NTT-MMP-2 was intensely expressed in a basolateral pattern. FL-MMP-2 and NTT-MMP-2 isoform expression as quantified by qPCR were both significantly elevated in renal biopsies of human diabetic nephropathy (12-fold and 3-fold, respectively). Conclusions The expression of both isoforms of MMP-2 was enhanced in an experimental model of diabetic nephropathy and in human diabetic nephropathy. Selective MMP-2 isoform inhibition could offer a novel approach for

  7. Caracterisation des proprietes acoustiques des materiaux poreux a cellules ouvertes et a matrice rigide ou souple

    Science.gov (United States)

    Salissou, Yacoubou

    L'objectif global vise par les travaux de cette these est d'ameliorer la caracterisation des proprietes macroscopiques des materiaux poreux a structure rigide ou souple par des approches inverses et indirectes basees sur des mesures acoustiques faites en tube d'impedance. La precision des approches inverses et indirectes utilisees aujourd'hui est principalement limitee par la qualite des mesures acoustiques obtenues en tube d'impedance. En consequence, cette these se penche sur quatre problemes qui aideront a l'atteinte de l'objectif global precite. Le premier probleme porte sur une caracterisation precise de la porosite ouverte des materiaux poreux. Cette propriete en est une de passage permettant de lier la mesure des proprietes dynamiques acoustiques d'un materiau poreux aux proprietes effectives de sa phase fluide decrite par les modeles semi-phenomenologiques. Le deuxieme probleme traite de l'hypothese de symetrie des materiaux poreux selon leur epaisseur ou un index et un critere sont proposes pour quantifier l'asymetrie d'un materiau. Cette hypothese est souvent source d'imprecision des methodes de caracterisation inverses et indirectes en tube d'impedance. Le critere d'asymetrie propose permet ainsi de s'assurer de l'applicabilite et de la precision de ces methodes pour un materiau donne. Le troisieme probleme vise a mieux comprendre le probleme de transmission sonore en tube d'impedance en presentant pour la premiere fois un developpement exact du probleme par decomposition d'ondes. Ce developpement permet d'etablir clairement les limites des nombreuses methodes existantes basees sur des tubes de transmission a 2, 3 ou 4 microphones. La meilleure comprehension de ce probleme de transmission est importante puisque c'est par ce type de mesures que des methodes permettent d'extraire successivement la matrice de transfert d'un materiau poreux et ses proprietes dynamiques intrinseques comme son impedance caracteristique et son nombre d'onde complexe. Enfin, le

  8. Kinetic evaluation of cell membrane hydrolysis during apoptosis by human isoforms of secretory phospholipase A2.

    OpenAIRE

    Olson, Erin D.; Nelson, Jennifer; Griffith, Katalyn; Nguyen, Thaothanh; Streeter, Michael; Wilson-Ashworth, Heather A.; Michael H Gelb; Judd, Allan M.; Bell, John D.

    2010-01-01

    Some isoforms of secretory phospholipase A2 (sPLA2) distinguish between healthy and damaged or apoptotic cells. This distinction reflects differences in membrane physical properties. Because various sPLA2 isoforms respond differently to properties of artificial membranes such as surface charge, they should also behave differently as these properties evolve during a dynamic physiological process such as apoptosis. To test this idea, S49 lymphoma cell death was induced by glucocorticoid (6–48 h...

  9. Role of ROCK Isoforms in Regulation of Stiffness Induced Myofibroblast Differentiation in Lung Fibrosis.

    Science.gov (United States)

    Htwe, Su S; Cha, Byung H; Yue, Kan; Khademhosseini, Ali; Knox, Alan J; Ghaemmaghami, Amir M

    2017-02-22

    Fibrosis is a major cause of progressive organ dysfunction in several chronic pulmonary diseases. Rho associated coiled-coil forming kinase (ROCK) has shown to be involved in myofibroblast differentiation driven by altered matrix stiffness in fibrotic state. There are two known ROCK isoforms in human, ROCK1 (ROKβ) and ROCK2 (ROKα), but specific role of each isoform in myofibroblast differentiation in lung fibrosis remains unknown. To study this, we developed a Gelatin methacryloyl (GelMA) hydrogel based culture system with different stiffness levels relevant to healthy and fibrotic lungs. We have shown that stiff matrix and not soft matrix, can induce myofibroblast differentiation with high αSMA expression. Furthermore, our data confirm that the inhibition of ROCK signalling by a pharmacological inhibitor (i.e. Y27632) attenuates stiffness induced αSMA expression and fibre assembly in myofibroblasts. To assess the role of ROCK isoforms in this process we used siRNA to knock down the expression of each isoform. Our data showed that knocking down either ROCK1 or ROCK2 did not result in a reduction in αSMA expression in myofibroblasts on stiff matrix as opposed to soft matrix where αSMA expression was reduced significantly. Paradoxically, on stiff matrix, the absence of one isoform (particularly ROCK2) exaggerated αSMA expression and led to thick fibre assembly. Moreover complete loss of αSMA fibre assembly was seen only in the absence of both ROCK isoforms suggesting that both isoforms are implicated in this process. Overall our results indicate the differential role of ROCK isoforms in myofibroblast differentiation on soft and stiff matrices.

  10. Recombinant erythropoietin in humans has a prolonged effect on circulating erythropoietin isoform distribution.

    Directory of Open Access Journals (Sweden)

    Niels Jacob Aachmann-Andersen

    Full Text Available The membrane-assisted isoform immunoassay (MAIIA quantitates erythropoietin (EPO isoforms as percentages of migrated isoforms (PMI. We evaluated the effect of recombinant human EPO (rhEPO on the distribution of EPO isoforms in plasma in a randomized, placebo-controlled, double-blinded, cross-over study. 16 healthy subjects received either low-dose Epoetin beta (5000 IU on days 1, 3, 5, 7, 9, 11 and 13; high-dose Epoetin beta (30.000 IU on days 1, 2 and 3 and placebo on days 5, 7, 9, 11 and 13; or placebo on all days. PMI on days 4, 11 and 25 was determined by interaction of N-acetyl glucosamine with the glycosylation dependent desorption of EPO isoforms. At day 25, plasma-EPO in both rhEPO groups had returned to values not different from the placebo group. PMI with placebo, reflecting the endogenous EPO isoforms, averaged 82.5 (10.3 % (mean (SD. High-dose Epoetin beta decreased PMI on days 4 and 11 to 31.0 (4.2% (p<0.00001 and 45.2 (7.3% (p<0.00001. Low-dose Epoetin beta decreased PMI on days 4 and 11 to 46.0 (12.8% (p<0.00001 and 46.1 (10.4% (p<0.00001. In both rhEPO groups, PMI on day 25 was still decreased (high-dose Epoetin beta: 72.9 (19.4% (p=0.029; low-dose Epoetin beta: 73.1 (17.8% (p=0.039. In conclusion, Epoetin beta leaves a footprint in the plasma-EPO isoform pattern. MAIIA can detect changes in EPO isoform distribution up til at least three weeks after administration of Epoetin beta even though the total EPO concentration has returned to normal.

  11. Modulation of estrogen receptor-beta isoforms by phytoestrogens in breast cancer cells.

    Science.gov (United States)

    Cappelletti, Vera; Miodini, Patrizia; Di Fronzo, Giovanni; Daidone, Maria Grazia

    2006-05-01

    High consumption of phytoestrogen-rich food correlates with reduced incidence of breast cancer. However, the effect of phytoestrogens on growth of pre-existing breast tumors presents concerns when planning the use of phytoestrogens as chemoprevention st rategy. Genistein, the active phytoestrogen in soy, displays weak estrogenic activity mediated by estrogen receptor (ER) with a preferential binding for the ER-beta species. However, no information is at present available on the interaction between phytoestrogens and the various isoforms generated by alternative splicing. In two human breast cancer cell lines, T47D and BT20, which express variable levels of ER-beta, the effect of genistein and quercetin was evaluated singly and in comparison with 17beta-estradiol, on mRNA expression of estrogen receptor-beta (ER-beta) isoforms evaluated by a triple primer RT-PCR assay. In T47D cells estradiol caused a 6-fold up-regulation of total ER-beta, and modified the relative expression pattern of the various isoforms, up-regulating the beta2 and down-regulating the beta5 isoform. Genistein up-regulated ER-beta2 and ER-beta1 in T47D cells, and after treatment the ER-beta2 isoform became prevalent, while in BT20 cells it almost doubled the percent contribution of ER-beta1 and ER-beta2 to total ER-beta. Quercetin did not alter the total levels nor the percent distribution of ER-beta isoforms in either cell line. Genistein, through the modulation of ER-beta isoform RNA expression inhibited estrogen-promoted cell growth, without interfering on estrogen-regulated transcription. ER-beta and its ER-beta mRNA isoforms may be involved in a self-limiting mechanism of estrogenic stimulation promoted either by the natural hormone or by weaker estrogen agonists like genistein.

  12. Robust stratification of breast cancer subtypes using differential patterns of transcript isoform expression.

    Directory of Open Access Journals (Sweden)

    Thomas P Stricker

    2017-03-01

    Full Text Available Breast cancer, the second leading cause of cancer death of women worldwide, is a heterogenous disease with multiple different subtypes. These subtypes carry important implications for prognosis and therapy. Interestingly, it is known that these different subtypes not only have different biological behaviors, but also have distinct gene expression profiles. However, it has not been rigorously explored whether particular transcriptional isoforms are also differentially expressed among breast cancer subtypes, or whether transcript isoforms from the same sets of genes can be used to differentiate subtypes. To address these questions, we analyzed the patterns of transcript isoform expression using a small set of RNA-sequencing data for eleven Estrogen Receptor positive (ER+ subtype and fourteen triple negative (TN subtype tumors. We identified specific sets of isoforms that distinguish these tumor subtypes with higher fidelity than standard mRNA expression profiles. We found that alternate promoter usage, alternative splicing, and alternate 3'UTR usage are differentially regulated in breast cancer subtypes. Profiling of isoform expression in a second, independent cohort of 68 tumors confirmed that expression of splice isoforms differentiates breast cancer subtypes. Furthermore, analysis of RNAseq data from 594 cases from the TCGA cohort confirmed the ability of isoform usage to distinguish breast cancer subtypes. Also using our expression data, we identified several RNA processing factors that were differentially expressed between tumor subtypes and/or regulated by estrogen receptor, including YBX1, YBX2, MAGOH, MAGOHB, and PCBP2. RNAi knock-down of these RNA processing factors in MCF7 cells altered isoform expression. These results indicate that global dysregulation of splicing in breast cancer occurs in a subtype-specific and reproducible manner and is driven by specific differentially expressed RNA processing factors.

  13. Kinetics of local and systemic isoforms of serum amyloid A in bovine mastitic milk

    DEFF Research Database (Denmark)

    Jacobsen, Stine; Niewold, T.A.; Kornalijnslijper, E.

    2005-01-01

    The aim of the present study was to characterise the serum amyloid A (SAA) response to intramammary inoculation of Escherichia coli and to examine the distribution of hepatically and extrahepatically pruduced SAA isoforms in plasma and milk fra cows with mastitis.......The aim of the present study was to characterise the serum amyloid A (SAA) response to intramammary inoculation of Escherichia coli and to examine the distribution of hepatically and extrahepatically pruduced SAA isoforms in plasma and milk fra cows with mastitis....

  14. Differential Regulation of Human Thymosin Beta 15 Isoforms by Transforming Growth Factor Beta 1

    Science.gov (United States)

    Banyard, Jacqueline; Barrows, Courtney; Zetter, Bruce R.

    2009-01-01

    We recently identified an additional isoform of human thymosin beta 15 (also known as NB-thymosin beta, gene name TMSB15A) transcribed from an independent gene, and designated TMSB15B. The purpose of this study was to investigate whether these isoforms were differentially expressed and functional. Our data show that the TMSB15A and TMSB15B isoforms have distinct expression patterns in different tumor cell lines and tissues. TMSB15A was expressed at higher levels in HCT116, DU145, LNCaP and LNCaP-LN3 cancer cells. In MCF-7, SKOV-3, HT1080 and PC-3MLN4 cells, TMSB15A and TMSB15B showed approximately equivalent levels of expression, while TMSB15B was the predominant isoform expressed in PC-3, MDA-MB-231, NCI-H322 and Caco-2 cancer cells. In normal human prostate and prostate cancer tissues, TMSB15A was the predominant isoform expressed. In contrast, normal colon and colon cancer tissue expressed predominantly TMSB15B. The two gene isoforms are also subject to different transcriptional regulation. Treatment of MCF-7 breast cancer cells with transforming growth factor beta 1 repressed TMSB15A expression but had no effect on TMSB15B. siRNA specific to the TMSB15B isoform suppressed cell migration of prostate cancer cells to epidermal growth factor, suggesting a functional role for this second isoform. In summary, our data reveal different expression patterns and regulation of a new thymosin beta 15 gene paralog. This may have important consequences in both tumor and neuronal cell motility. PMID:19296525

  15. Myosin heavy-chain isoforms in the flight and leg muscles of hummingbirds and zebra finches.

    Science.gov (United States)

    Velten, Brandy P; Welch, Kenneth C

    2014-06-01

    Myosin heavy chain (MHC) isoform complement is intimately related to a muscle's contractile properties, yet relatively little is known about avian MHC isoforms or how they may vary with fiber type and/or the contractile properties of a muscle. The rapid shortening of muscles necessary to power flight at the high wingbeat frequencies of ruby-throated hummingbirds and zebra finches (25-60 Hz), along with the varied morphology and use of the hummingbird hindlimb, provides a unique opportunity to understand how contractile and morphological properties of avian muscle may be reflected in MHC expression. Isoforms of the hummingbird and zebra finch flight and hindlimb muscles were electrophoretically separated and compared with those of other avian species representing different contractile properties and fiber types. The flight muscles of the study species operate at drastically different contraction rates and are composed of different histochemically defined fiber types, yet each exhibited the same, single MHC isoform corresponding to the chicken adult fast isoform. Thus, despite quantitative differences in the contractile demands of flight muscles across species, this isoform appears necessary for meeting the performance demands of avian powered flight. Variation in flight muscle contractile performance across species may be due to differences in the structural composition of this conserved isoform and/or variation within other mechanically linked proteins. The leg muscles were more varied in their MHC isoform composition across both muscles and species. The disparity in hindlimb MHC expression between hummingbirds and the other species highlights previously observed differences in fiber type composition and thrust production during take-off. Copyright © 2014 the American Physiological Society.

  16. Recombinant erythropoietin in humans has a prolonged effect on circulating erythropoietin isoform distribution.

    Science.gov (United States)

    Aachmann-Andersen, Niels Jacob; Just Christensen, Søren; Lisbjerg, Kristian; Oturai, Peter; Meinild-Lundby, Anne-Kristine; Holstein-Rathlou, Niels-Henrik; Lundby, Carsten; Vidiendal Olsen, Niels

    2014-01-01

    The membrane-assisted isoform immunoassay (MAIIA) quantitates erythropoietin (EPO) isoforms as percentages of migrated isoforms (PMI). We evaluated the effect of recombinant human EPO (rhEPO) on the distribution of EPO isoforms in plasma in a randomized, placebo-controlled, double-blinded, cross-over study. 16 healthy subjects received either low-dose Epoetin beta (5000 IU on days 1, 3, 5, 7, 9, 11 and 13); high-dose Epoetin beta (30.000 IU on days 1, 2 and 3 and placebo on days 5, 7, 9, 11 and 13); or placebo on all days. PMI on days 4, 11 and 25 was determined by interaction of N-acetyl glucosamine with the glycosylation dependent desorption of EPO isoforms. At day 25, plasma-EPO in both rhEPO groups had returned to values not different from the placebo group. PMI with placebo, reflecting the endogenous EPO isoforms, averaged 82.5 (10.3) % (mean (SD)). High-dose Epoetin beta decreased PMI on days 4 and 11 to 31.0 (4.2)% (p<0.00001) and 45.2 (7.3)% (p<0.00001). Low-dose Epoetin beta decreased PMI on days 4 and 11 to 46.0 (12.8)% (p<0.00001) and 46.1 (10.4)% (p<0.00001). In both rhEPO groups, PMI on day 25 was still decreased (high-dose Epoetin beta: 72.9 (19.4)% (p=0.029); low-dose Epoetin beta: 73.1 (17.8)% (p=0.039)). In conclusion, Epoetin beta leaves a footprint in the plasma-EPO isoform pattern. MAIIA can detect changes in EPO isoform distribution up til at least three weeks after administration of Epoetin beta even though the total EPO concentration has returned to normal.

  17. Genomic organization and the tissue distribution of alternatively spliced isoforms of the mouse Spatial gene

    Directory of Open Access Journals (Sweden)

    Mattei Marie-Geneviève

    2004-07-01

    Full Text Available Abstract Background The stromal component of the thymic microenvironment is critical for T lymphocyte generation. Thymocyte differentiation involves a cascade of coordinated stromal genes controlling thymocyte survival, lineage commitment and selection. The "Stromal Protein Associated with Thymii And Lymph-node" (Spatial gene encodes a putative transcription factor which may be involved in T-cell development. In the testis, the Spatial gene is also expressed by round spermatids during spermatogenesis. Results The Spatial gene maps to the B3-B4 region of murine chromosome 10 corresponding to the human syntenic region 10q22.1. The mouse Spatial genomic DNA is organised into 10 exons and is alternatively spliced to generate two short isoforms (Spatial-α and -γ and two other long isoforms (Spatial-δ and -ε comprising 5 additional exons on the 3' site. Here, we report the cloning of a new short isoform, Spatial-β, which differs from other isoforms by an additional alternative exon of 69 bases. This new exon encodes an interesting proline-rich signature that could confer to the 34 kDa Spatial-β protein a particular function. By quantitative TaqMan RT-PCR, we have shown that the short isoforms are highly expressed in the thymus while the long isoforms are highly expressed in the testis. We further examined the inter-species conservation of Spatial between several mammals and identified that the protein which is rich in proline and positive amino acids, is highly conserved. Conclusions The Spatial gene generates at least five alternative spliced variants: three short isoforms (Spatial-α, -β and -γ highly expressed in the thymus and two long isoforms (Spatial-δ and -ε highly expressed in the testis. These alternative spliced variants could have a tissue specific function.

  18. Biological Effects of TMPRSS2/ERG Fusion Isoforms in Human Prostate Cancer

    Science.gov (United States)

    2009-02-01

    TITLE: Biological Effects of TMPRSS2/ERG Fusion Isoforms in Human Prostate Cancer PRINCIPAL INVESTIGATOR: Jianghua Wang, M.D...6 JAN 2009 / / /4. TITLE AND SUBTITLE Biological Effects of TMPRSS2/ERG Fusion Isoforms in Human Prostate Cancer 5a. CONTRACT NUMBER W81XWH...quantitative RT-PCR arrays we have identified candidate mediators of these phenotypic effects . We propose to extend these studies to primary prostate epithelial

  19. The impact of tropomyosins on actin filament assembly is isoform specific.

    Science.gov (United States)

    Janco, Miro; Bonello, Teresa T; Byun, Alex; Coster, Adelle C F; Lebhar, Helene; Dedova, Irina; Gunning, Peter W; Böcking, Till

    2016-07-01

    Tropomyosin (Tpm) is an α helical coiled-coil dimer that forms a co-polymer along the actin filament. Tpm is involved in the regulation of actin's interaction with binding proteins as well as stabilization of the actin filament and its assembly kinetics. Recent studies show that multiple Tpm isoforms also define the functional properties of distinct actin filament populations within a cell. Subtle structural variations within well conserved Tpm isoforms are the key to their functional specificity. Therefore, we purified and characterized a comprehensive set of 8 Tpm isoforms (Tpm1.1, Tpm1.12, Tpm1.6, Tpm1.7, Tpm1.8, Tpm2.1, Tpm3.1, and Tpm4.2), using well-established actin co-sedimentation and pyrene fluorescence polymerization assays. We observed that the apparent affinity (Kd(app)) to filamentous actin varied in all Tpm isoforms between ∼0.1-5 μM with similar values for both, skeletal and cytoskeletal actin filaments. The data did not indicate any correlation between affinity and size of Tpm molecules, however high molecular weight (HMW) isoforms Tpm1.1, Tpm1.6, Tpm1.7 and Tpm2.1, showed ∼3-fold higher cooperativity compared to low molecular weight (LMW) isoforms Tpm1.12, Tpm1.8, Tpm3.1, and Tpm4.2. The rate of actin filament elongation in the presence of Tpm2.1 increased, while all other isoforms decreased the elongation rate by 27-85 %. Our study shows that the biochemical properties of Tpm isoforms are finely tuned and depend on sequence variations in alternatively spliced regions of Tpm molecules.

  20. Kinetic properties of alternatively spliced isoforms of laccase-2 from Tribolium castaneum and Anopheles gambiae.

    Science.gov (United States)

    Gorman, Maureen J; Sullivan, Lucinda I; Nguyen, Thi D T; Dai, Huaien; Arakane, Yasuyuki; Dittmer, Neal T; Syed, Lateef U; Li, Jun; Hua, Duy H; Kanost, Michael R

    2012-03-01

    Laccase-2 is a highly conserved multicopper oxidase that functions in insect cuticle pigmentation and tanning. In many species, alternative splicing gives rise to two laccase-2 isoforms. A comparison of laccase-2 sequences from three orders of insects revealed eleven positions at which there are conserved differences between the A and B isoforms. Homology modeling suggested that these eleven residues are not part of the substrate binding pocket. To determine whether the isoforms have different kinetic properties, we compared the activity of laccase-2 isoforms from Tribolium castaneum and Anopheles gambiae. We partially purified the four laccases as recombinant enzymes and analyzed their ability to oxidize a range of laccase substrates. The predicted endogenous substrates tested were dopamine, N-acetyldopamine (NADA), N-β-alanyldopamine (NBAD) and dopa, which were detected in T. castaneum previously and in A. gambiae as part of this study. Two additional diphenols (catechol and hydroquinone) and one non-phenolic substrate (2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid)) were also tested. We observed no major differences in substrate specificity between the A and B isoforms. Dopamine, NADA and NBAD were oxidized with catalytic efficiencies ranging from 51 to 550 min⁻¹ mM⁻¹. These results support the hypothesis that dopamine, NADA and NBAD are endogenous substrates for both isoforms of laccase-2. Catalytic efficiencies associated with dopa oxidation were low, ranging from 8 to 30 min⁻¹ mM⁻¹; in comparison, insect tyrosinase oxidized dopa with a catalytic efficiency of 201 min⁻¹ mM⁻¹. We found that dopa had the highest redox potential of the four endogenous substrates, and this property of dopa may explain its poor oxidation by laccase-2. We conclude that laccase-2 splice isoforms are likely to oxidize the same substrates in vivo, and additional experiments will be required to discover any isoform-specific functions.

  1. TENDANCES ACTUELLES EN MARKETING DES SERVICES

    Directory of Open Access Journals (Sweden)

    George NICULESCU

    2010-09-01

    Full Text Available Les activités de services sont difficiles à gérer dans le cadre du marketing-mix traditionnel car, contrairement aux biens tangibles produits la qualité des services est très dépendante de facteurs personnels. De même, la nature intangible des services n’est pas prise en compte dans la plupart des analyses du marketing-mix. Par exemple, la distribution physique ne coïncide pas avec la distribution d’éléments invisibles et la politique de communication de l’approche traditionnelle du marketingmix (4P’s ne reconnaît pas la communication des services établie sur le lieu de rencontre entre le personnel de contact de la firme et les clients. Même si les opinions sont partagées et que le débat n’est pas clos, on peut considérer que des éléments supplémentaires s’imposent pour élaborer un marketing qui permette de satisfaire les attentes des parties contractantes de la façon la plus satisfaisante possible

  2. Wiktor Stoczkowski, Des hommes, des dieux et des extraterrestres. Ethnologie d’une croyance moderne

    OpenAIRE

    Lepoutre, David

    2006-01-01

    Voici un ouvrage qui nous fait entrer de plain-pied dans une subculture contemporaine généralement perçue comme marginale et qui s’avère pourtant être d’une importance sociale considérable. Il s’agit de l’univers, exotique s’il en est, des parasciences et de la pensée occultiste. Le lecteur curieux y trouvera un ensemble de développements remarquablement bien documentés et ordonnés sur un certain nombre de sujets et de thèmes liés à cette pensée contemporaine dont les ramifications tentaculai...

  3. Expression of mdr isoforms in mice during estrous cycle and under hormone stimulation

    Directory of Open Access Journals (Sweden)

    Marion Schiengold

    2006-01-01

    Full Text Available The multidrug resistance (MDR phenotype is associated with the expression of P-glycoprotein (Pgp, coded by the multigenic mdr family. Mice present the isoforms mdr1 and mdr3, which are responsible for multidrug resistance, and mdr2, that is involved in the transport of phospholipids. mdr1 expression has more recently been associated also with the secretion of steroid hormones. This work presents an RT-PCR analysis of the expression of mdr isoforms, in several organs of mice during different phases of the estrous cycle. Additionally, females were ovariectomized, submitted to different hormone treatments, and their uterus was analyzed for the expression of mdr isoforms. The results show that in the adrenal gland and ovaries mdr1 is the main isoform during proestrus, and that progesterone or a combination of progesterone and estrogen induce the expression of all mdr isoforms in the uterus of ovariectomized females. We suggest that the functions of mdr1 and mdr3 are overlapping, that mdr3 may be the more efficient isoform in the detoxification function, and that mdr1 may be more closely related to the secretion of steroid hormones.

  4. C/EBPβ Isoforms Expression in the Rat Brain during the Estrous Cycle

    Directory of Open Access Journals (Sweden)

    Valeria Hansberg-Pastor

    2015-01-01

    Full Text Available The CCAAT/enhancer-binding protein beta (C/EBPβ is a transcription factor expressed in different areas of the brain that regulates the expression of several genes involved in cell differentiation and proliferation. This protein has three isoforms (LAP1, LAP2, and LIP with different transcription activation potential. The role of female sex hormones in the expression pattern of C/EBPβ isoforms in the rat brain has not yet been described. In this study we demonstrate by western blot that the expression of the three C/EBPβ isoforms changes in different brain areas during the estrous cycle. In the cerebellum, LAP2 content diminished on diestrus and proestrus and LIP content diminished on proestrus and estrus days. In the prefrontal cortex, LIP content was higher on proestrus and estrus days. In the hippocampus, LAP isoforms presented a switch on diestrus day, since LAP1 content was the highest while that of LAP2 was the lowest. The LAP2 isoform was the most abundant one in all the three brain areas. The LAP/LIP ratio changed throughout the cycle and was tissue specific. These results suggest that C/EBPβ isoforms expression changes in a tissue-specific manner in the rat brain due to the changes in sex steroid hormone levels presented during the estrous cycle.

  5. Characterization of 14-3-3 isoforms expressed in the Echinococcus granulosus pathogenic larval stage.

    Science.gov (United States)

    Teichmann, Aline; Vargas, Daiani M; Monteiro, Karina M; Meneghetti, Bruna V; Dutra, Cristine S; Paredes, Rodolfo; Galanti, Norbel; Zaha, Arnaldo; Ferreira, Henrique B

    2015-04-01

    The 14-3-3 protein family of eukaryotic regulators was studied in Echinococcus granulosus, the causative agent of cystic hydatid disease. These proteins mediate important cellular processes in eukaryotes and are expected to play important roles in parasite biology. Six isoforms of E. granulosus 14-3-3 genes and proteins (Eg14-3-3.1-6) were analyzed, and their phylogenetic relationships were established with bona fide 14-3-3 orthologous proteins from eukaryotic species. Eg14-3-3 isoforms with previous evidence of expression (Eg14-3-3.1-4) in E. granulosus pathogenic larval stage (metacestode) were cloned, and recombinant proteins were used for functional studies. These protein isoforms were detected in different components of E. granulosus metacestode, including interface components with the host. The roles that are played by Eg14-3-3 proteins in parasite biology were inferred from the repertoires of interacting proteins with each isoform, as assessed by gel overlay, cross-linking, and affinity chromatography assays. A total of 95 Eg14-3-3 protein ligands were identified by mass spectrometry. Eg14-3-3 isoforms have shared partners (44 proteins), indicating some overlapping functions; however, they also bind exclusive partners (51 proteins), suggesting Eg14-3-3 functional specialization. These ligand repertoires indicate the involvement of Eg14-3-3 proteins in multiple biochemical pathways in the E. granulosus metacestode and note some degree of isoform specialization.

  6. Profiling alternatively spliced mRNA isoforms for prostate cancer classification

    Directory of Open Access Journals (Sweden)

    Fan Jian-Bing

    2006-04-01

    Full Text Available Abstract Background Prostate cancer is one of the leading causes of cancer illness and death among men in the United States and world wide. There is an urgent need to discover good biomarkers for early clinical diagnosis and treatment. Previously, we developed an exon-junction microarray-based assay and profiled 1532 mRNA splice isoforms from 364 potential prostate cancer related genes in 38 prostate tissues. Here, we investigate the advantage of using splice isoforms, which couple transcriptional and splicing regulation, for cancer classification. Results As many as 464 splice isoforms from more than 200 genes are differentially regulated in tumors at a false discovery rate (FDR of 0.05. Remarkably, about 30% of genes have isoforms that are called significant but do not exhibit differential expression at the overall mRNA level. A support vector machine (SVM classifier trained on 128 signature isoforms can correctly predict 92% of the cases, which outperforms the classifier using overall mRNA abundance by about 5%. It is also observed that the classification performance can be improved using multivariate variable selection methods, which take correlation among variables into account. Conclusion These results demonstrate that profiling of splice isoforms is able to provide unique and important information which cannot be detected by conventional microarrays.

  7. Molecular modeling study on tunnel behavior in different histone deacetylase isoforms.

    Directory of Open Access Journals (Sweden)

    Sundarapandian Thangapandian

    Full Text Available Histone deacetylases (HDACs have emerged as effective therapeutic targets in the treatment of various diseases including cancers as these enzymes directly involved in the epigenetic regulation of genes. However the development of isoform-selective HDAC inhibitors has been a challenge till date since all HDAC enzymes possess conserved tunnel-like active site. In this study, using molecular dynamics simulation we have analyzed the behavior of tunnels present in HDAC8, 10, and 11 enzymes of class I, II, and IV, respectively. We have identified the equivalent tunnel forming amino acids in these three isoforms and found that they are very much conserved with subtle differences to be utilized in selective inhibitor development. One amino acid, methionine of HDAC8, among six tunnel forming residues is different in isoforms of other classes (glutamic acid (E in HDAC10 and leucine (L in HDAC 11 based on which mutations were introduced in HDAC11, the less studied HDAC isoform, to observe the effects of this change. The HDAC8-like (L268M mutation in the tunnel forming residues has almost maintained the deep and narrow tunnel as present in HDAC8 whereas HDAC10-like (L268E mutation has changed the tunnel wider and shallow as observed in HDAC10. These results explained the importance of the single change in the tunnel formation in different isoforms. The observations from this study can be utilized in the development of isoform-selective HDAC inhibitors.

  8. Divergent roles for thyroid hormone receptor β isoforms in the endocrine axis and auditory system

    Science.gov (United States)

    Abel, E. Dale; Boers, Mary-Ellen; Pazos-Moura, Carmen; Moura, Egberto; Kaulbach, Helen; Zakaria, Marjorie; Lowell, Bradford; Radovick, Sally; Liberman, M. Charles; Wondisford, Fredric

    1999-01-01

    Thyroid hormone receptors (TRs) modulate various physiological functions in many organ systems. The TRα and TRβ isoforms are products of 2 distinct genes, and the β1 and β2 isoforms are splice variants of the same gene. Whereas TRα1 and TRβ1 are widely expressed, expression of the TRβ2 isoform is mainly limited to the pituitary, triiodothyronine-responsive TRH neurons, the developing inner ear, and the retina. Mice with targeted disruption of the entire TRβ locus (TRβ-null) exhibit elevated thyroid hormone levels as a result of abnormal central regulation of thyrotropin, and also develop profound hearing loss. To clarify the contribution of the TRβ2 isoform to the function of the endocrine and auditory systems in vivo, we have generated mice with targeted disruption of the TRβ2 isoform. TRβ2-null mice have preserved expression of the TRα and TRβ1 isoforms. They develop a similar degree of central resistance to thyroid hormone as TRβ-null mice, indicating the important role of TRβ2 in the regulation of the hypothalamic-pituitary-thyroid axis. Growth hormone gene expression is marginally reduced. In contrast, TRβ2-null mice exhibit no evidence of hearing impairment, indicating that TRβ1 and TRβ2 subserve divergent roles in the regulation of auditory function. PMID:10430610

  9. Role of acyl carrier protein isoforms in plant lipid metabolism: Progress report

    Energy Technology Data Exchange (ETDEWEB)

    Ohlrogge, J.B.

    1989-01-01

    Previous research from my lab has revealed that several higher plant species have multiple isoforms of acyl carrier protein (ACP) and therefore this trait appears highly conserved among higher plants. This level of conservation suggests that the existence of ACP isoforms is not merely the results of neutral gene duplications. We have developed techniques to examine a wider range of species. Acyl carrier proteins can be labelled very specifically and to high specific activity using H-palmitate and the E. coli enzyme acyl-ACP synthetase. Isoforms were then resolved by western blotting and native PAGE of H-palmitate labelled ACP's. Multiple isoforms of ACP were observed the leaf tissue of the monocots Avena sativa and Hordeum vulgare and dicots including Arabidopsis thallina, Cuphea wrightii, and Brassica napus. Lower vascular plants including the cycad, Dioon edule, Ginkgo biloba, the gymnosperm Pinus, the fern Anernia phyllitidis and Psilotum nudum, the most primitive known extant vascular plant, were also found to have multiple ACP isoforms as were the nonvascular liverwort, Marchantia and moss, Polytrichum. Therefore, the development of ACP isoforms occurred early in evolution. However, the uniellular alge Chlamydomonas and Dunaliella and the photosynthetic cyanobacteria Synechocystis and Agmnellum have only a single elecrophotetic form of ACP. Thus, multiple forms of ACP do not occur in all photosynthetic organisms but may be associated with multicellular plants.

  10. Distribution of tropomyosin isoforms in different types of single fibers isolated from bovine skeletal muscles.

    Science.gov (United States)

    Oe, M; Ojima, K; Nakajima, I; Chikuni, K; Shibata, M; Muroya, S

    2016-08-01

    To clarify the relationship between myosin heavy chain (MyHC) isoforms and tropomyosin (TPM) isoforms in single fibers, 64 single fibers were isolated from each of bovine three muscles (masseter, semispinalis and semitendinosus). mRNA expressions of MyHC and TPM isoforms were analyzed by real-time PCR. All single fibers from the masseter expressed MyHC-slow. The fibers from the semispinalis expressed both MyHC-slow and 2a. The fibers from the semitendinosus expressed MyHC-slow, 2a and 2x. TPM-1 and TPM-2 were co-expressed in 2a and 2x type fibers, and TPM-2 and TPM-3 were co-expressed in slow type fibers. The expression pattern of TPM isoforms in each fiber type was similar between fibers isolated from different muscles. These results suggest that TPM-1 and TPM-3 isoforms correspond to the function of 2a or 2x type fibers and slow type fibers, respectively, with TPM-2 in common. Furthermore, the patterns of MyHC and TPM isoform combinations did not vary among single fibers isolated from the individual muscles examined.

  11. Two novel human NUMB isoforms provide a potential link between development and cancer.

    Science.gov (United States)

    Karaczyn, Aldona; Bani-Yaghoub, Mahmud; Tremblay, Roger; Kubu, Chris; Cowling, Rebecca; Adams, Tamara L; Prudovsky, Igor; Spicer, Douglas; Friesel, Robert; Vary, Calvin; Verdi, Joseph M

    2010-12-01

    We previously identified four functionally distinct human NUMB isoforms. Here, we report the identification of two additional isoforms and propose a link between the expression of these isoforms and cancer. These novel isoforms, NUMB5 and NUMB6, lack exon 10 and are expressed in cells known for polarity and migratory behavior, such as human amniotic fluid cells, glioblastoma and metastatic tumor cells. RT-PCR and luciferase assays demonstrate that NUMB5 and NUMB6 are less antagonistic to NOTCH signaling than other NUMB isoforms. Immunocytochemistry analyses show that NUMB5 and NUMB6 interact and complex with CDC42, vimentin and the CDC42 regulator IQGAP1 (IQ (motif) GTPase activating protein 1). Furthermore, the ectopic expression of NUMB5 and NUMB6 induces the formation of lamellipodia (NUMB5) and filopodia (NUMB6) in a CDC42- and RAC1-dependent manner. These results are complemented by in vitro and in vivo studies, demonstrating that NUMB5 and NUMB6 alter the migratory behavior of cells. Together, these novel isoforms may play a role in further understanding the NUMB function in development and cancer.

  12. Two novel human NUMB isoforms provide a potential link between development and cancer

    Directory of Open Access Journals (Sweden)

    Prudovsky Igor

    2010-12-01

    Full Text Available Abstract We previously identified four functionally distinct human NUMB isoforms. Here, we report the identification of two additional isoforms and propose a link between the expression of these isoforms and cancer. These novel isoforms, NUMB5 and NUMB6, lack exon 10 and are expressed in cells known for polarity and migratory behavior, such as human amniotic fluid cells, glioblastoma and metastatic tumor cells. RT-PCR and luciferase assays demonstrate that NUMB5 and NUMB6 are less antagonistic to NOTCH signaling than other NUMB isoforms. Immunocytochemistry analyses show that NUMB5 and NUMB6 interact and complex with CDC42, vimentin and the CDC42 regulator IQGAP1 (IQ (motif GTPase activating protein 1. Furthermore, the ectopic expression of NUMB5 and NUMB6 induces the formation of lamellipodia (NUMB5 and filopodia (NUMB6 in a CDC42- and RAC1-dependent manner. These results are complemented by in vitro and in vivo studies, demonstrating that NUMB5 and NUMB6 alter the migratory behavior of cells. Together, these novel isoforms may play a role in further understanding the NUMB function in development and cancer.

  13. Glutamic acid decarboxylase isoform distribution in transgenic mouse septum: an anti-GFP immunofluorescence study.

    Science.gov (United States)

    Verimli, Ural; Sehirli, Umit S

    2016-09-01

    The septum is a basal forebrain region located between the lateral ventricles in rodents. It consists of lateral and medial divisions. Medial septal projections regulate hippocampal theta rhythm whereas lateral septal projections are involved in processes such as affective functions, memory formation, and behavioral responses. Gamma-aminobutyric acidergic neurons of the septal region possess the 65 and 67 isoforms of the enzyme glutamic acid decarboxylase. Although data on the glutamic acid decarboxylase isoform distribution in the septal region generally appears to indicate glutamic acid decarboxylase 67 dominance, different studies have given inconsistent results in this regard. The aim of this study was therefore to obtain information on the distributions of both of these glutamic acid decarboxylase isoforms in the septal region in transgenic mice. Two animal groups of glutamic acid decarboxylase-green fluorescent protein knock-in transgenic mice were utilized in the experiment. Brain sections from the region were taken for anti-green fluorescent protein immunohistochemistry in order to obtain estimated quantitative data on the number of gamma-aminobutyric acidergic neurons. Following the immunohistochemical procedures, the mean numbers of labeled cells in the lateral and medial septal nuclei were obtained for the two isoform groups. Statistical analysis yielded significant results which indicated that the 65 isoform of glutamic acid decarboxylase predominates in both lateral and medial septal nuclei (unpaired two-tailed t-test p glutamic acid decarboxylase isoform 65 in the septal region in glutamic acid decarboxylase-green fluorescent protein transgenic mice.

  14. Structure- and isoform-specific glucuronidation of six curcumin analogs.

    Science.gov (United States)

    Lu, Danyi; Liu, Hui; Ye, Wencai; Wang, Ying; Wu, Baojian

    2017-04-01

    1. In the present study, we aimed to characterize the glucuronidation of six curcumin analogs (i.e. RAO-3, RAO-8, RAO-9, RAO-18, RAO-19, and RAO-23) derived from galangal using human liver microsomes (HLM) and twelve expressed UGT enzymes. 2. Formation of glucuronide was confirmed using high-resolution mass spectrometry. Single glucuronide metabolite was generated from each of six curcumin analogs. The fragmentation patterns were analyzed and were found to differ significantly between alcoholic and phenolic glucuronides. 3. All six curcumin analogs except one (RAO-23) underwent significant glucuronidation in HLM and expressed UGT enzymes. In general, the methoxy group (close to the phenolic hydroxyl group) enhanced the glucuronidation liability of the curcumin analogs. 4. UGT1A9 and UGT2B7 were primarily responsible for the glucuronidation of two alcoholic analogs (RAO-3 and RAO-18). By contrast, UGT1A9 and four UGT2Bs (UGT2B4, 2B7, 2B15 and 2B17) played important roles in conjugating three phenolic analogs (RAO-8, RAO-9, and RAO-19). Interestingly, the conjugated double bonds system (in the aliphatic chain) was crucial to the substrate selectivity of gastrointestinal UGTs (i.e. UGT1A7, 1A8 and 1A10). 5. In conclusion, glucuronidation of six curcumin analogs from galangal were structure- and isoform-specific. The knowledge should be useful in identifying a curcumin analog with improved metabolic property.

  15. 127 Impact des rejets urbains et industriels sur la qualité des eaux ...

    African Journals Online (AJOL)

    PR BOKO

    rejets industriels et urbains sur l'évolution de la qualité des eaux de surface et ... 1,0205= 11820, sa principale activité est l'agriculture, visible surtout dans la partie Sud là .... Ils sont également indicateurs d'une pollution par des eaux usées.

  16. Marine Traffic Project, un outil d’observation des routes et des ports maritimes

    Directory of Open Access Journals (Sweden)

    Hervé Théry

    2011-12-01

    Full Text Available Le site Marine Traffic fournit, gratuitement et en temps réel, des informations sur les mouvements des navires dans le monde entier. Il fait partie d’un projet universitaire qui a pour but la collecte et la diffusion de ces données en vue de leur exploitation dans divers domaines…

  17. Services de transfert des savoirs

    Directory of Open Access Journals (Sweden)

    Albert Simard

    2009-03-01

    Full Text Available Le présent article décrit un modèle visant à faciliter la compréhension du processus de transfert du contenu depuis sa source jusqu’à son utilisation. Un groupe de travail de Ressources naturelles Canada a étudié la nature du transfert des savoirs à l’aide d’un modèle de services, afin de découvrir et comprendre les modèles et processus sous-jacents. Les services de savoir sont des programmes qui créent et fournissent des produits organisationnels basés sur un contenu et ayant une valeur intégrée. Ces programmes sont destinés à être utilisés en interne ou transférés pour répondre aux souhaits et besoins d’un utilisateur externe. Les critères de conception du modèle sont les suivants : indépendance par rapport au contenu, concentration sur l’organisation, évolutivité, deux pilotes et deux niveaux de résolution. Le modèle est basé sur une chaîne de valeur dans laquelle la valeur est intégrée, déplacée ou extraite. Un système de services de savoir se divise en neuf étapes : générer, transformer, gérer, utiliser en interne, transférer, ajouter de la valeur, utiliser professionnellement, utiliser personnellement et évaluer. Les services de savoir sont fournis par l’intermédiaire d’un spectre de distribution allant de complexe à accessible. À chaque région du spectre de distribution correspond une série d’attributs qui influencent la manière dont les services sont fournis.This paper describes a model intended to increase understanding of how content is transferred from its source to its use. A Natural Resources Canada task group explored the nature of knowledge transfer with a services paradigm to discover and understand underlying patterns and processes. Knowledge Services are programs that produce and pro-vide content-based organizational outputs with embedded value that are intended to be used internally or transferred to meet external user wants or needs. Model design criteria

  18. Effets perturbateurs endocriniens des pesticides organochlores.

    Science.gov (United States)

    Charlier, C; Plomteux, G

    2002-01-01

    Xenoestrogens such organochlorine pesticides are known to induce changes in reproductive development, function or behaviour in wildlife. Because these compounds are able to modify the estrogens metabolism, or to compete with estradiol for binding to the estrogen receptor, it may be possible that these products affect the risk of developing impaired fertility, precocious puberty or some kinds of cancer in man. Le plus ancien récit de lutte contre la pollution remonte à une légende indienne racontant que la divinité Sing-bonga était incommodée par les émanations des fours dans lesquels les Asuras fondaient leurs métaux (1). Evidemment depuis, la problématique n-a cessé de s-accroître et la contamination de la Terre par de nombreux polluants est devenue aujourd-hui un problème majeur de notre Société. La protection de notre environnement est une question capitale qui doit être respectée malgré la pression économique actuelle et qui ne cessera de croître au cours des prochaines années même si l-identification objective et indiscutable de ce qui est essentiel - donc devant être prioritairement garanti sur la planète - est difficile à cerner (2). « Un oiseau en mauvais état ne pond pas de bons oeufs » disait un proverbe grec. Mais ce n-est qu-à partir de la seconde moitié du XXème siècle que les toxicologues ont commencé à identifier les effets qu-avaient entraînés à l-échelle mondiale les pollutions émises aux XIXème siècle sur la faune sauvage et sur le cheptel (3). L-histoire contemporaine des pesticides industriels commence vers 1874 (synthèse des organochlorés) et se poursuit tout au long de ces 2 siècles en passant par la synthèse des organophosphorés (1950), des carbamates (1970) et des pyréthroïdes (1975) (4). Le dichlorodiphényltrichloroéthane (DDT) a été synthétisé pour la première fois par un étudiant en cours de préparation de sa thèse de doctorat : Othmer Zeidler. La production, reprise par les

  19. Micro Blog:un monde des rumeurs%Micro Blog : un monde des rumeurs

    Institute of Scientific and Technical Information of China (English)

    杨洁

    2012-01-01

    Quel est le divertissement le plus populaire sur Internet depuis ao(u)t 2009 en Chine ? Ce serait le Micro Blog du site web Sina.Avec 100 millions d'utilisateurs,ce Micro Blog est une plate-forme où l'on peut partager,diffuser et obtenir des informations immédiatement.En comparaison d'autres micro blogs tels que twitter et facebook,celui de Sina nous offre plus de services.Avec 140 mots,nous pouvons bien exprimer au moins une idée en chinois.De plus,en diffusant des critiques,des photos,des vidéos et des chansons,chacun devient jourr(n)aliste en observant et enregistrant les nouvelles de la vie.

  20. Inhibition of carbonic anhydrase isoforms I, II, IX and XII with novel Schiff bases: identification of selective inhibitors for the tumor-associated isoforms over the cytosolic ones.

    Science.gov (United States)

    Sarikaya, Busra; Ceruso, Mariangela; Carta, Fabrizio; Supuran, Claudiu T

    2014-11-01

    A series of new Schiff bases was obtained from sulfanilamide, 3-fluorosulfanilamide or 4-(2-aminoethyl)-benzenesulfonamide and aromatic/heterocyclic aldehydes incorporating both hydrophobic and hydrophilic moieties. The obtained sulfonamides were investigated as inhibitors of four physiologically relevant carbonic anhydrase (CA, EC 4.2.1.1) isoforms, the cytosolic CA I and II, as well as the transmembrane, tumor-associated CA IX and XII. Most derivatives were medium potency or weak hCA I/II inhibitors, but several of them showed nanomolar affinity for CA IX and/or XII, making them an interesting example of isoform-selective compounds. The nature of the aryl/hetaryl moiety present in the initial aldehyde was the main factor influencing potency and isoform selectivity. The best and most CA IX-selective compounds incorporated moieties such as 4-methylthiophenyl, 4-cyanophenyl-, 4-(2-pyridyl)-phenyl and the 4-aminoethylbenzenesulfonamide scaffold. The best hCA XII inhibitors, also showing selectivity for this isoform, incorporated 2-methoxy-4-nitrophenyl-, 2,3,5,6-tetrafluorophenyl and 4-(2-pyridyl)-phenyl functionalities and were also derivatives of 4-aminoethylbenzenesulfonamide. The sulfanilamide and 3-fluorosulfanilamide derived Schiff bases were less active compared to the corresponding 4-aminoethyl-benzenesulfonamide derivatives. As hCA IX/XII selective inhibition is attractive for obtaining antitumor agents/diagnostic tools with a new mechanism of action, compounds of the type described here may be considered interesting preclinical candidates.

  1. Vom Bürger zum Kunden. Der Strukturwandel des Bildungssystems und der demokratischen Kultur in Deutschland

    OpenAIRE

    Zymek, Bernd

    2004-01-01

    Der Artikel analysiert aus einer bildungs- und sozialhistorischen Perspektive die Ursachen und Konsequenzen des auffälligen Begriffswandels und Strategiewechsels in den bildungspolitischen Debatten Deutschlands. die aktuelle Konjunktur ökonomischer Begriffe und betriebswirtschaftlicher Strategien wird im Zusammenhang des langfristigen Strukturwandels des deutschen Bildungssystems, des Funktionsverlusts des Berechtigungswesens und der Expansion kommerzieller Bildungsangebote im Umfeld des staa...

  2. Selective expression of myosin IC Isoform A in mouse and human cell lines and mouse prostate cancer tissues.

    Science.gov (United States)

    Ihnatovych, Ivanna; Sielski, Neil L; Hofmann, Wilma A

    2014-01-01

    Myosin IC is a single headed member of the myosin superfamily. We recently identified a novel isoform and showed that the MYOIC gene in mammalian cells encodes three isoforms (isoforms A, B, and C). Furthermore, we demonstrated that myosin IC isoform A but not isoform B exhibits a tissue specific expression pattern. In this study, we extended our analysis of myosin IC isoform expression patterns by analyzing the protein and mRNA expression in various mammalian cell lines and in various prostate specimens and tumor tissues from the transgenic mouse prostate (TRAMP) model by immunoblotting, qRT-PCR, and by indirect immunohistochemical staining of paraffin embedded prostate specimen. Analysis of a panel of mammalian cell lines showed an increased mRNA and protein expression of specifically myosin IC isoform A in a panel of human and mouse prostate cancer cell lines but not in non-cancer prostate or other (non-prostate-) cancer cell lines. Furthermore, we demonstrate that myosin IC isoform A expression is significantly increased in TRAMP mouse prostate samples with prostatic intraepithelial neoplasia (PIN) lesions and in distant site metastases in lung and liver when compared to matched normal tissues. Our observations demonstrate specific changes in the expression of myosin IC isoform A that are concurrent with the occurrence of prostate cancer in the TRAMP mouse prostate cancer model that closely mimics clinical prostate cancer. These data suggest that elevated levels of myosin IC isoform A may be a potential marker for the detection of prostate cancer.

  3. Expression of 14-3-3 protein isoforms in mouse oocytes, eggs and ovarian follicular development

    Directory of Open Access Journals (Sweden)

    De Santanu

    2012-01-01

    Full Text Available Abstract Background The 14-3-3 (YWHA proteins are a highly conserved, ubiquitously expressed family of proteins. Seven mammalian isoforms of 14-3-3 are known (β, γ, ε, ζ, η, τ and, σ. These proteins associate with many intracellular proteins involved in a variety of cellular processes including regulation of the cell cycle, metabolism and protein trafficking. We are particularly interested in the role of 14-3-3 in meiosis in mammalian eggs and the role 14-3-3 proteins may play in ovarian function. Therefore, we examined the expression of 14-3-3 proteins in mouse oocyte and egg extracts by Western blotting after polyacrylamide gel electrophoresis, viewed fixed cells by indirect immunofluorescence, and examined mouse ovarian cells by immunohistochemical staining to study the expression of the different 14-3-3 isoforms. Results We have determined that all of the mammalian 14-3-3 isoforms are expressed in mouse eggs and ovarian follicular cells including oocytes. Immunofluorescence confocal microscopy of isolated oocytes and eggs confirmed the presence of all of the isoforms with characteristic differences in some of their intracellular localizations. For example, some isoforms (β, ε, γ, and ζ are expressed more prominently in peripheral cytoplasm compared to the germinal vesicles in oocytes, but are uniformly dispersed within eggs. On the other hand, 14-3-3η is diffusely dispersed in the oocyte, but attains a uniform punctate distribution in the egg with marked accumulation in the region of the meiotic spindle apparatus. Immunohistochemical staining detected all isoforms within ovarian follicles, with some similarities as well as notable differences in relative amounts, localizations and patterns of expression in multiple cell types at various stages of follicular development. Conclusions We found that mouse oocytes, eggs and follicular cells within the ovary express all seven isoforms of the 14-3-3 protein. Examination of the

  4. La gestion des eaux souterraines et des steppes en Syrie : une ouverture internationale sous contrôle

    OpenAIRE

    Saadé-Sbeih, Myriam; Jaubert, Ronald; Al-Dbiyat, Mohamed

    2014-01-01

    À partir de la fin des années 1990, dans un contexte d’ouverture à l’international, les autorités syriennes adoptèrent une posture conforme au discours des organisations internationales de développement en redéfinissant les politiques d’exploitation des eaux souterraines et des parcours steppiques. Si les programmes mis en œuvre semblent dans un premier temps correspondre à l’adoption des principes et instruments de régulation et de gestion des ressources naturelles promus par les organisatio...

  5. Multistatic Surveillance and Reconnaissance: Sensor, Signals and Data Fusion (Surveillance et Reconnaissance Multistatiques : Fusion des capteurs, des signaux et des donnees)

    Science.gov (United States)

    2009-04-01

    capteurs , des signaux et des données) Research and Technology Organisation (NATO) BP 25, F-92201 Neuilly-sur-Seine Cedex, France RTO-EN-SET-133...this new paradigm, each radar/sonar can receive and process its own signal and/or the signal of other local sources. The application of bi-/multistatic...Multistatiques : Fusion des capteurs , des signaux et des données) The material in this publication was assembled to support a Lecture Series under the

  6. Aimant PS: controle de l'etat des sertissages internes des PFW mesures effectues surl'ensemble des aimants du PS pendant le grand arret 2004/2006

    CERN Document Server

    Ottaviani, J

    2005-01-01

    Les sertissages (douilles), utilisés pour raccorder les méplats du circuit principal des PFW à leurs câbles d’alimentation, présentent parfois des défauts de réalisation qui entraînent une résistance électrique anormale. Pour mettre en évidence ces défauts, la résistance des 4 sertissages des nappes polaires est mesurée. Les nappes sont alimentées avec un courant efficace nominal de 80A.Les mesures sont effectuées entre le bornier de connexions des 4 câbles d’alimentation des PFW et les circuits auxiliaires correspondants (Pick-ups brasés sur les enroulements). De ces mesures (câble d’alimentation et une courte partie du circuit principal) est déduite la valeur de la résistance du sertissage qui en assure la liaison. A la mesure des résistances des sertissages des nappes polaires supérieures s’ajoute la résistance des câbles qui assurent la liaison entre borniers. Dans le cadre de la rénovation des aimants du PS, les 100 unités on été mesurées.

  7. Dans le tourbillon des particules

    CERN Document Server

    Zito, Marco

    2015-01-01

    Accélérateurs géants, détecteurs complexes, particules énigmatiques... La physique subatomique peut sembler bien intimidante pour le novice. Et pourtant, qui n a jamais entendu parler du boson de Higgs et du CERN, le laboratoire européen où il a été découvert en 2012 ? Nul besoin d être un spécialiste pour comprendre de quoi il s agit. Aujourd hui, une théorie extraordinairement élégante, le Modèle Standard, décrit tous les résultats des expériences dans le domaine. Trente-sept particules élémentaires et quatre forces fondamentales : c est tout ce dont nous avons besoin pour expliquer la matière et l Univers ! Ce livre, destiné à un large public, raconte sans équations le long parcours qui a abouti au Modèle Standard. Ce parcours, parfois sinueux, a été entamé lorsque les Grecs anciens, et peut-être d autres avant eux, ont imaginé que la matière est composée de petites « billes ». Il faudra attendre plusieurs siècles pour qu on réalise que la matière, à l échelle micros...

  8. Running Club - Nocturne des Evaux

    CERN Multimedia

    Running club

    2017-01-01

    Les coureurs du CERN sont encore montés sur les plus hautes marches du podium lors de la course interentreprises. Cette course d’équipe qui se déroule de nuit et par équipe de 3 à 4 coureurs est unique dans la région de par son originalité : départ groupé toutes les 30 secondes, les 3 premiers coureurs doivent passer la ligne d’arrivée ensemble. Double victoire pour le running club a la nocturne !!!! 1ère place pour les filles et 22e au classement général; 1ère place pour l'équipe mixte et 4e au général, battant par la même occasion le record de l'épreuve en mixte d'environ 1 minute; 10e place pour l'équipe homme. Retrouvez tous les résultats sur http://www.chp-geneve.ch/web-cms/index.php/nocturne-des-evaux

  9. Identification of alternatively translated Tetherin isoforms with differing antiviral and signaling activities.

    Directory of Open Access Journals (Sweden)

    Luis J Cocka

    2012-09-01

    Full Text Available Tetherin (BST-2/CD317/HM1.24 is an IFN induced transmembrane protein that restricts release of a broad range of enveloped viruses. Important features required for Tetherin activity and regulation reside within the cytoplasmic domain. Here we demonstrate that two isoforms, derived by alternative translation initiation from highly conserved methionine residues in the cytoplasmic domain, are produced in both cultured human cell lines and primary cells. These two isoforms have distinct biological properties. The short isoform (s-Tetherin, which lacks 12 residues present in the long isoform (l-Tetherin, is significantly more resistant to HIV-1 Vpu-mediated downregulation and consequently more effectively restricts HIV-1 viral budding in the presence of Vpu. s-Tetherin Vpu resistance can be accounted for by the loss of serine-threonine and tyrosine motifs present in the long isoform. By contrast, the l-Tetherin isoform was found to be an activator of nuclear factor-kappa B (NF-κB signaling whereas s-Tetherin does not activate NF-κB. Activation of NF-κB requires a tyrosine-based motif found within the cytoplasmic tail of the longer species and may entail formation of l-Tetherin homodimers since co-expression of s-Tetherin impairs the ability of the longer isoform to activate NF-κB. These results demonstrate a novel mechanism for control of Tetherin antiviral and signaling function and provide insight into Tetherin function both in the presence and absence of infection.

  10. Differential CARM1 Isoform Expression in Subcellular Compartments and among Malignant and Benign Breast Tumors.

    Directory of Open Access Journals (Sweden)

    David Shlensky

    Full Text Available Coactivator-associated arginine methyltransferase 1 (CARM1 is a coactivator for ERα and cancer-relevant transcription factors, and can methylate diverse cellular targets including histones. CARM1 is expressed in one of two alternative splice isoforms, full-length CARM1 (CARM1FL and truncated CARM1 (CARM1ΔE15. CARM1FL and CARM1ΔE15 function differently in transcriptional regulation, protein methylation, and mediation of pre-mRNA splicing in cellular models.To investigate the functional roles and the prognosis potential of CARM1 alternative spliced isoforms in breast cancer, we used recently developed antibodies to detect differential CARM1 isoform expression in subcellular compartments and among malignant and benign breast tumors.Immunofluorescence in MDA-MB-231 and BG-1 cell lines demonstrated that CARM1ΔE15 is the dominant isoform expressed in the cytoplasm, and CARM1FL is more nuclear localized. CARM1ΔE15 was found to be more sensitive to Hsp90 inhibition than CARM1FL, indicating that the truncated isoform may be the oncogenic form. Clinical cancer samples did not have significantly higher expression of CARM1FL or CARM1ΔE15 than benign breast samples at the level of mRNA or histology. Furthermore neither CARM1FL nor CARM1ΔE15 expression correlated with breast cancer molecular subtypes, tumor size, or lymph node involvement.The analysis presented here lends new insights into the possible oncogenic role of CARM1ΔE15. This study also demonstrates no obvious association of CARM1 isoform expression and clinical correlates in breast cancer. Recent studies, however, have shown that CARM1 expression correlates with poor prognosis, indicating a need for further studies of both CARM1 isoforms in a large cohort of breast cancer specimens.

  11. Differential dynamics of RAS isoforms in GDP- and GTP-bound states.

    Science.gov (United States)

    Kapoor, Abhijeet; Travesset, Alex

    2015-06-01

    RAS subfamily proteins regulates cell growth promoting signaling processes by cycling between active (GTP-bound) and inactive (GDP-bound) states. Different RAS isoforms, though structurally similar, exhibit functional specificity and are associated with different types of cancers and developmental disorders. Understanding the dynamical differences between the isoforms is crucial for the design of inhibitors that can selectively target a particular malfunctioning isoform. In this study, we provide a comprehensive comparison of the dynamics of all the three RAS isoforms (HRAS, KRAS, and NRAS) using extensive molecular dynamics simulations in both the GDP- (total of 3.06 μs) and GTP-bound (total of 2.4 μs) states. We observed significant differences in the dynamics of the isoforms, which rather interestingly, varied depending on the type of the nucleotide bound and the simulation temperature. Both SwitchI (Residues 25-40) and SwitchII (Residues 59-75) differ significantly in their flexibility in the three isoforms. Furthermore, Principal Component Analysis showed that there are differences in the conformational space sampled by the GTP-bound RAS isoforms. We also identified a previously unreported pocket, which opens transiently during MD simulations, and can be targeted to regulate nucleotide exchange reaction or possibly interfere with membrane localization. Further, we present the first simulation study showing GDP destabilization in the wild-type RAS protein. The destabilization of GDP/GTP occurred only in 1/50 simulations, emphasizing the need of guanine nucleotide exchange factors (GEFs) to accelerate such an extremely unfavorable process. This observation along with the other results presented in this article further support our previously hypothesized mechanism of GEF-assisted nucleotide exchange. © 2015 Wiley Periodicals, Inc.

  12. Development and characterization of human monoclonal antibodies that neutralize multiple TGFβ isoforms.

    Science.gov (United States)

    Bedinger, Daniel; Lao, Llewelyn; Khan, Shireen; Lee, Steve; Takeuchi, Toshihiko; Mirza, Amer M

    2016-01-01

    Transforming growth factor (TGF)β levels are elevated in, and drive the progression of, numerous disease states such as advanced metastatic cancer and systemic and ocular fibrosis. There are 3 main isoforms, TGFβ1, 2, and 3. As multiple TGFβ isoforms are involved in disease processes, maximal therapeutic efficacy may require neutralization of 2 or more of the TGFβ isoforms. Fully human antibody phage display libraries were used to discover a number of antibodies that bind and neutralize various combinations of TGFβ1, 2 or 3. The primary panning did not yield any uniformly potent pan-isoform neutralizing antibodies; therefore, an antibody that displayed potent TGFβ 1, 2 inhibition, but more modest affinity versus TGFβ3, was affinity matured by shuffling with a light chain sub-library and further screening. This process yielded a high affinity pan-isoform neutralizing clone. Antibodies were analyzed and compared by binding affinity, as well as receptor and epitope competition by surface plasmon resonance methods. The antibodies were also shown to neutralize TGFβ effects in vitro in 3 assays: 1) interleukin (IL)-4 induced HT-2 cell proliferation; 2) TGFβ-mediated IL-11 release by A549 cells; and 3) decreasing SMAD2 phosphorylation in Detroit 562 cells. The antibodies' potency in these in vitro assays correlated well with their isoform-specific affinities. Furthermore, the ability of the affinity-matured clone to decrease tumor burden in a Detroit 562 xenograft study was superior to that of the parent clone. This affinity-matured antibody acts as a very potent inhibitor of all 3 main isoforms of TGFβ and may have utility for therapeutic intervention in human disease.

  13. Dictionnaire d'analyse et de gestion des risques

    CERN Document Server

    Desroches, Alain

    2005-01-01

    Ce dictionnaire présente les principaux termes utilisés en analyse et gestion des risques, pour tous les secteurs d'activité industrielle ou de service. Il permet de clarifier des terminologies existantes présentant des variations plus ou moins importantes et parfois des contradictions de sens. Il propose : premièrement, la définition des auteurs, définition correspondant à l'usage le plus courant du terme , deuxièmement, les différences avec les terminologies existantes (essentiellement des normes) , troisièmement, des compléments à la définition, lorsque cela semble nécessaire et notamment lorsqu'il est important de préciser le contexte d'utilisation du terme , en dernier lieu, des exemples pratiques d'application, lorsque cela peut apporter un éclairage complémentaire pertinent.

  14. Refuge development report : Des Lacs Migratory Waterfowl Refuge

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — Report on the development of Des Lacs National Wildlife Refuge. This report introduces the physical and biological characteristics of the Des Lacs area. This report...

  15. [Des Lacs National Wildlife Refuge habitat improvement to combat botulism

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This documents discusses the possibilities of ditching the Upper Des Lacs lake as a means of combating botulism on the Des Lacs National Wildlife Refuge.

  16. Stabilisation de la formule des traces tordue

    CERN Document Server

    Moeglin, Colette

    2016-01-01

    Ce travail en deux volumes donne la preuve de la stabilisation de la formule des trace tordue. Stabiliser la formule des traces tordue est la méthode la plus puissante connue actuellement pour comprendre l'action naturelle du groupe des points adéliques d'un groupe réductif, tordue par un automorphisme, sur les formes automorphes de carré intégrable de ce groupe. Cette compréhension se fait en réduisant le problème, suivant les idées de Langlands, à des groupes plus petits munis d'un certain nombre de données auxiliaires; c'est ce que l'on appelle les données endoscopiques. L'analogue non tordu a été résolu par J. Arthur et dans ce livre on suit la stratégie de celui-ci. Publier ce travail sous forme de livre permet de le rendre le plus complet possible. Les auteurs ont repris la théorie de l'endoscopie tordue développée par R. Kottwitz et D. Shelstad et par J.-P. Labesse. Ils donnent tous les arguments des démonstrations même si nombre d'entre eux se trouvent déjà dans les travaux d'Ar...

  17. Positionsbestimmung des Unternehmens: Interne und externe Analyse

    Science.gov (United States)

    Bergmann, Lars; Crespo, Isabel; Portmann, Stefan

    Die Initiierung und Lenkung von Maßnahmen zur integrierten Modernisierung zielen auf die Verbesserung der Wettbewerbsfähigkeit eines Unternehmens ab. Damit diese Maßnahmen zielgerichtet die Wettbewerbsfähigkeit verbessern können, ist Wissen über die bestehende Wettbewerbsfähigkeit sowie über die bestehenden Fähigkeiten eine zentrale Voraussetzung. Eine zielgerichtete Auswahl problemadäquater Maßnahmen zur Verbesserung der Wettbewerbsfähigkeit bedarf daher im Vorfeld einer Bewertung der aktuellen Situation des Unternehmens im Sinne einer Positionsbestimmung. Erst wenn die internen Stärken und Schwächen sowie die externen Chancen und Risiken identifiziert sind, kann ein ganzheitliches Bild von der Position eines Unternehmens in seiner Umwelt gewonnen werden. Auf Basis der Kenntnisse über die Position des Unternehmens können anschließend zielgerichtet Maßnahmen ausgewählt werden, die einen Beitrag zur Verbesserung der Wettbewerbsfähigkeit des Unternehmens haben. Damit kommt der Positionsbestimmung als initialer Schritt des Prozesses der strategischen Unternehmensplanung eine zentrale Bedeutung im Rahmen der integrierten Modernisierung zu. Erfolgt die Auswahl von Maßnahmen ohne eine vorherige Positionsbestimmung, also lediglich auf Basis drängender Probleme, so besteht die Gefahr einer unbedachten und nur auf das "hier und heute“ bezogenen Schwerpunktbildung ohne Berücksichtigung der mittel- und langfristigen Ziele des Unternehmens.

  18. MODELACIÓN DE LA ESTRUCTURA JERÁRQUICA DE MACROINVERTEBRADOS BENTÓNICOS A TRAVÉS DE REDES NEURONALES ARTIFICIALES Modeling of the Hierarchical Structure of Freshwater Macroinvertebrates Using Artificial Neural Networks

    Directory of Open Access Journals (Sweden)

    CLAUDIA RICO

    Full Text Available El estudio de la estructura jerárquica de comunidades ecológicas, se ha sintetizado de manera regular a través de técnicas multivariadas de ordenación o clasificación. Sin embargo, al contarse actualmente con herramientas analíticas de computación bioinspirada provenientes de la inteligencia artificial, existe la oportunidad de establecer modelos ecológicos, con características deseables como flexibilidad, exactitud, robustez y confiabilidad. En este contexto, esta investigación utilizó dos métodos computacionales de utilidad en ecoinformática, referidos a redes neuronales artificiales (RNARs para la modelación de la estructura jerárquica de una comunidad de macroinvertebrados bentónicos en términos de auto-organización y predicción. El primer método de modelación consistió en un mapa de auto-organización (MAU, una herramienta de aprendizaje no supervisado que clasificó las especies de macroinvertebrados; este MAU tomó en la capa de entrada la abundancia de cada taxa, y en la de salida proyectó su clasificación en 15 unidades y cuatro agrupamientos jerárquicos. La segunda RNA, correspondió a un Perceptrón multicapa de alimentación adelantada con algoritmo de retropropagación, que modeló separadamente la riqueza y la abundancia de Ephemeroptera, Coleoptera y Trichoptera (ECT, en función de nueve variables fisicoquímicas; la arquitectura del perceptrón correspondió a una constitución de nueve, siete, y una neurona en las capas de entrada, intermedia y salida, respectivamente. Los resultados sugieren que las RNARs utilizadas evidenciaron tanto los patrones jerárquicos, como los de riqueza y abundancia de ECT de manera adecuada, al tiempo que facilitaron el análisis de los datos y el entendimiento de la dinámica de la comunidad de macroinvertebrados, objeto de estudio.The study of hierarchical structures of ecological communities has been synthesized in an ordinary way by means of multivariated techniques of

  19. Two isoforms of trimming glucosidase II exist in mammalian tissues and cell lines but not in yeast and insect cells.

    Science.gov (United States)

    Ziak, M; Meier, M; Etter, K S; Roth, J

    2001-01-12

    We previously cloned glucosidase II and provided in vivo evidence for its involvement in protein folding quality control. DNA-sequencing of different clones demonstrated the existence of two isoforms of glucosidase II which differed by 66 nucleotides due to alternative splicing. The existence of two enzyme isoforms in various organs of pig and rat as well as human, bovine, rat, and mouse cell lines could be demonstrated by RT-PCR and Western blotting. Furthermore, the two isoforms of glucosidase II could be detected in embryonic and postnatal rat kidney and liver. In yeast, Saccharomyces cerevisiae, and in insects, Drosophila S2 cells, only one isoforms of the enzyme was detectable. The ubiquitous occurrence of the two glucosidase II isoforms in mammalian tissues and cell lines might be indicative of a special function of each isoform.

  20. LA CONTRIBUTION DES SAVOIRS DANS LPERFORMANCE D’ENTREPRISE

    OpenAIRE

    Lachachi, Abdelhaq

    2014-01-01

    Cette étude traite la problématique de la contribution des savoirs dans la performance d’entreprise. En d’autres termes, quel est l’impact des pratiques du management des connaissances (catalyseurs et processus) dans la performance organisationnelle ? Plusieurs études ont montré que les catalyseurs du management des connaissances (Culture organisationnelle, structure organisationnelle, leadership, stratégie et vision et les technologies de l’information et de la communication) ...

  1. L’impact de la participation bancaire dans le capital des entreprises sur la politique de distribution des dividendes

    Directory of Open Access Journals (Sweden)

    Naoel Mezgani

    2013-11-01

    Full Text Available L’objectif de ce papier est de tester l’incidence de la participation bancaire dans le capital des entreprises non financières sur la politique de distribution des dividendes. En utilisant des données de panel des trente entreprises tunisiennes durant la période de 1999 à 2004, nous vérifions si en détenant le capital et la dette, la banque a la possibilité de contraindre la distribution des dividendes pour garantir le remboursement de ses fonds prêtés. Les résultats empiriques obtenus ne confirment pas l’impact significatif des banques actionnaires majoritaires et principaux créditeurs sur la distribution des dividendes dans les entreprises tunisiennes. Ces dernières soutiennent surtout la stabilité du niveau des dividendes payés d’une année à une autre.

  2. Molecular mechanical differences between isoforms of contractile actin in the presence of isoforms of smooth muscle tropomyosin.

    Directory of Open Access Journals (Sweden)

    Lennart Hilbert

    2013-10-01

    Full Text Available The proteins involved in smooth muscle's molecular contractile mechanism - the anti-parallel motion of actin and myosin filaments driven by myosin heads interacting with actin - are found as different isoforms. While their expression levels are altered in disease states, their relevance to the mechanical interaction of myosin with actin is not sufficiently understood. Here, we analyzed in vitro actin filament propulsion by smooth muscle myosin for [Formula: see text]-actin ([Formula: see text]A, [Formula: see text]-actin-tropomyosin-[Formula: see text] ([Formula: see text]A-Tm[Formula: see text], [Formula: see text]-actin-tropomyosin-[Formula: see text] ([Formula: see text]A-Tm[Formula: see text], [Formula: see text]-actin ([Formula: see text]A, [Formula: see text]-actin-tropomyosin-[Formula: see text] ([Formula: see text]A-Tm[Formula: see text], and [Formula: see text]-actin-tropomoysin-[Formula: see text] ([Formula: see text]A-Tm[Formula: see text]. Actin sliding analysis with our specifically developed video analysis software followed by statistical assessment (Bootstrapped Principal Component Analysis indicated that the in vitro motility of [Formula: see text]A, [Formula: see text]A, and [Formula: see text]A-Tm[Formula: see text] is not distinguishable. Compared to these three 'baseline conditions', statistically significant differences ([Formula: see text] were: [Formula: see text]A-Tm[Formula: see text] - actin sliding velocity increased 1.12-fold, [Formula: see text]A-Tm[Formula: see text] - motile fraction decreased to 0.96-fold, stop time elevated 1.6-fold, [Formula: see text]A-Tm[Formula: see text] - run time elevated 1.7-fold. We constructed a mathematical model, simulated actin sliding data, and adjusted the kinetic parameters so as to mimic the experimentally observed differences: [Formula: see text]A-Tm[Formula: see text] - myosin binding to actin, the main, and the secondary myosin power stroke are accelerated, [Formula: see text

  3. Distinct pharmacological properties and distribution in neurons and endocrine cells of two isoforms of the human vesicular monoamine transporter.

    OpenAIRE

    Erickson, J.D.; Schafer, M K; Bonner, T I; Eiden, L. E.; Weihe, E.

    1996-01-01

    A second isoform of the human vesicular monoamine transporter (hVMAT) has been cloned from a pheochromocytoma cDNA library. The contribution of the two transporter isoforms to monoamine storage in human neuroendocrine tissues was examined with isoform-specific polyclonal antibodies against hVMAT1 and hVMAT2. Central, peripheral, and enteric neurons express only VMAT2. VMAT1 is expressed exclusively in neuroendocrine, including chromaffin and enterochromaffin, cells. VMAT1 and VMAT2 are coexpr...

  4. Dichotomy of short and long thymic stromal lymphopoietin isoforms in inflammatory disorders of the bowel and skin

    Science.gov (United States)

    Fornasa, Giulia; Tsilingiri, Katerina; Caprioli, Flavio; Botti, Fiorenzo; Mapelli, Marina; Meller, Stephan; Kislat, Andreas; Homey, Bernhard; Di Sabatino, Antonio; Sonzogni, Angelica; Viale, Giuseppe; Diaferia, Giuseppe; Gori, Alessandro; Longhi, Renato; Penna, Giuseppe; Rescigno, Maria

    2015-01-01

    Background Thymic stromal lymphopoietin (TSLP) is a cytokine with pleiotropic functions in the immune system. It has been associated with allergic reactions in the skin and lungs but also homeostatic tolerogenic responses in the thymus and gut. Objective In human subjects TSLP is present in 2 isoforms, short and long. Here we wanted to investigate the differential expression of the TSLP isoforms and discern their biological implications under homeostatic or inflammatory conditions. Methods We evaluated the expression of TSLPs in tissues from healthy subjects, patients with ulcerative colitis, patients with celiac disease, and patients with atopic dermatitis and on epithelial cells and keratinocytes under steady-state conditions or after stimulation. We then tested the immune activity of TSLP isoforms both in vitro and in vivo. Results We showed that TSLP isoforms are responsible for 2 opposite immune functions. The short isoform is expressed under steady-state conditions and exerts anti-inflammatory activities by affecting the capacity of PBMCs and dendritic cells to produce inflammatory cytokines. Moreover, the short isoform TSLP ameliorates experimental colitis in mice and prevents endotoxin shock. The long isoform of TSLP is proinflammatory and is only expressed during inflammation. The isoforms are differentially regulated by pathogenic bacteria, such as Salmonella species and adhesive-invasive Escherichia coli. Conclusions We have solved the dilemma of TSLP being both homeostatic and inflammatory. The TSLP isoform ratio is altered during several inflammatory disorders, with strong implications in disease treatment and prevention. Indeed, targeting of the long isoform of TSLP at the C-terminal portion, which is common to both isoforms, might lead to unwanted side effects caused by neutralization of the homeostatic short isoform. PMID:26014813

  5. The Effect of Exercise Training on Skeletal Muscle Glucose Transorter Isoform GLUT4 Concentration in the Obese Zucker Rat

    Science.gov (United States)

    1991-05-01

    NUMBERS The Effect of Exercise Training on Skeletal Muscle Glucose Transorter Isoform GLUT4 Concentration in the Obese Zucker Rat 6. AUTHOR(S) Eric A...Zr) THE EFFECT OF EXERCISE TRAINING ON SKELETAL MUSCLE GLUCOSE TRANSPORTER ISOFORM GLUT4 CONCENTRATION IN THE OBESE ZUCKER RAT by Eric Anthony Banks...laboratory for their help. Eric A. Banks v ABSTRACT THE EFFECT OF EXERCISE TRAINING ON SKELETAL MUSCLE GLUCOSE TRANSPORTER ISOFORM GLUT4 CONCENTRATION IN

  6. ordination et classification de la vegetation des zones humides du ...

    African Journals Online (AJOL)

    USER

    Ivoire appartiennent à la ... géographiques (Gauch, 1982 ; Kent et Coker, ... Le climat de la région est du type sub-équatorial, .... L'influence des ..... efficaces pour la partition des groupes de relevés ... les changements induits par des facteurs.

  7. Inhibition de Phytophthora palmivora, agent de pourriture brune des ...

    African Journals Online (AJOL)

    les pratiques culturales, la sélection des variétés résistances et l'utilisation des antagonistes ... will assess for efficacy biocontrol candidates from cacao agrosystems on the evolution of disease. ..... °C. Après 7 jours d'incubation, la lecture des.

  8. Statutes of limitations: the special problem of DES suits.

    Science.gov (United States)

    Feigin, C A

    1981-01-01

    In 1971, medical studies determined that DES causes a rare type of vaginal cancer in a small number of daughters of mothers who took DES during pregnancy. Subsequently, medical studies determined that exposure to DES can cause other vaginal abnormalities in the daughters, some of which may be precancerous. As a result of these discoveries, many lawsuits have been filed by these daughters against DES manufacturers. Many DES suits may be barred by statutes of limitations, both because the number of years between the daughters' exposure to DES in utero and the discovery that DES can cause injuries exceeds the statutory period, and because the cancer or other injuries caused by DES may not develop for many additional years. This Note discusses two methods that DES plaintiffs may be able to use to overcome the potential statutes of limitations bar: the discovery rule, and state provisions which toll the statute of limitations for minors. The Note contends that courts should apply an expanded discovery rule to DES suits to avoid the unfair result of barring a claim before the plaintiff could have known that she had a cause of action. In addition, the Note argues that the injury which causes the statute of limitations to begin to run in DES suits should not be rigidly defined. Finally, the Note urges that courts allow eligible DES plaintiffs to take advantage of applicable state provisions that toll the statute of limitations for minors.

  9. Les risques industriels et le prix des logements

    DEFF Research Database (Denmark)

    Grislain-Letrémy, Céline; Katossky, Arthur

    2013-01-01

    Le prix des logements peut diminuer du fait de la proximité d’installations industrielles. Cet effet dépend de la perception du risque par les riverains et est donc potentiellement modifié par des événements changeant la perception du risque, tels que les plans de prévention des risques technolog...

  10. Computational Estimates of Binding Affinities for Estrogen Receptor Isoforms in Rainbow Trout

    CERN Document Server

    Shyu, Conrad

    2009-01-01

    Molecular dynamics simulations are performed to determine the binding affinities between the hormone 17 beta-estradiol (E2) and different estrogen receptor (ER) isoforms in the rainbow trout (Oncorhynchus mykiss). Previous studies have demonstrated that a recent, unique gene duplication of the ER alpha subtype created two isoforms ER alpha 1 and ER alpha 2, and an early secondary split of ER beta produced two distinct isoforms of ER beta 1 and ER beta 2 based on the phylogenetic analysis. The objective of our computational studies is to provide insight into the underlying evolutionary selection pressure on the ER isoforms. Our results show that E2 binds preferentially to ER alpha 1. This finding corresponds to the experimental results as the ERs evolved from gene duplication events are frequently free from selective pressure and should exhibit no deleterious effects. The E2, however, only binds slightly better to ER beta 2. Both isoforms remain competitive. This finding reflects the fact that since ER beta 2 ...

  11. MAPA distinguishes genotype-specific variability of highly similar regulatory protein isoforms in potato tuber.

    Science.gov (United States)

    Hoehenwarter, Wolfgang; Larhlimi, Abdelhalim; Hummel, Jan; Egelhofer, Volker; Selbig, Joachim; van Dongen, Joost T; Wienkoop, Stefanie; Weckwerth, Wolfram

    2011-07-01

    Mass Accuracy Precursor Alignment is a fast and flexible method for comparative proteome analysis that allows the comparison of unprecedented numbers of shotgun proteomics analyses on a personal computer in a matter of hours. We compared 183 LC-MS analyses and more than 2 million MS/MS spectra and could define and separate the proteomic phenotypes of field grown tubers of 12 tetraploid cultivars of the crop plant Solanum tuberosum. Protein isoforms of patatin as well as other major gene families such as lipoxygenase and cysteine protease inhibitor that regulate tuber development were found to be the primary source of variability between the cultivars. This suggests that differentially expressed protein isoforms modulate genotype specific tuber development and the plant phenotype. We properly assigned the measured abundance of tryptic peptides to different protein isoforms that share extensive stretches of primary structure and thus inferred their abundance. Peptides unique to different protein isoforms were used to classify the remaining peptides assigned to the entire subset of isoforms based on a common abundance profile using multivariate statistical procedures. We identified nearly 4000 proteins which we used for quantitative functional annotation making this the most extensive study of the tuber proteome to date.

  12. Segmental distribution of myosin heavy chain isoforms within single muscle fibers.

    Science.gov (United States)

    Zhang, Ming; Gould, Maree

    2017-02-18

    Despite many studies looking at the distribution of myosin heavy chain (MHC) isoforms across a transverse section of muscle, knowledge of MHC distribution along the longitudinal axis of a single skeletal muscle fiber has been relatively overlooked. Immunocytochemistry was performed on serial sections of rat extensor digitorum longus (EDL) muscle to identify MHC types I, IIA, IIX, IIY and IIB. Sixteen fascicles which contained a total of 362 fibers were randomly and systematically sampled from the 3 EDL muscles. All MHC type I and type II isoforms were expressed. Segmental expression occurred within a very limited segment. MHC isoform expression followed the accepted traditional order from I&cenveo_unknown_entity_wingdings_F0F3;IIA&cenveo_unknown_entity_wingdings_F0F3;IIX&cenveo_unknown_entity_wingdings_F0F3;IIB, however in some samples expression of an isoform was circumvented from IIB to I or from I to IIB directly. Segmental distribution of MHC isoforms along a single muscle fiber may be due to the myonuclear domain. This article is protected by copyright. All rights reserved.

  13. Silencing GFAP isoforms in astrocytoma cells disturbs laminin-dependent motility and cell adhesion.

    Science.gov (United States)

    Moeton, Martina; Kanski, Regina; Stassen, Oscar M J A; Sluijs, Jacqueline A; Geerts, Dirk; van Tijn, Paula; Wiche, Gerhard; van Strien, Miriam E; Hol, Elly M

    2014-07-01

    Glial fibrillary acidic protein (GFAP) is an intermediate filament protein expressed in astrocytes and neural stem cells. The GFAP gene is alternatively spliced, and expression of GFAP is highly regulated during development, on brain damage, and in neurodegenerative diseases. GFAPα is the canonical splice variant and is expressed in all GFAP-positive cells. In the human brain, the alternatively spliced transcript GFAPδ marks specialized astrocyte populations, such as subpial astrocytes and the neurogenic astrocytes in the human subventricular zone. We here show that shifting the GFAP isoform ratio in favor of GFAPδ in astrocytoma cells, by selectively silencing the canonical isoform GFAPα with short hairpin RNAs, induced a change in integrins, a decrease in plectin, and an increase in expression of the extracellular matrix component laminin. Together, this did not affect cell proliferation but resulted in a significantly decreased motility of astrocytoma cells. In contrast, a down-regulation of all GFAP isoforms led to less cell spreading, increased integrin expression, and a >100-fold difference in the adhesion of astrocytoma cells to laminin. In summary, isoform-specific silencing of GFAP revealed distinct roles of a specialized GFAP network in regulating the interaction of astrocytoma cells with the extracellular matrix through laminin.-Moeton, M., Kanski, R., Stassen, O. M. J. A., Sluijs, J. A., Geerts, D., van Tijn, P., Wiche, G., van Strien, M. E., Hol, E. M. Silencing GFAP isoforms in astrocytoma cells disturbs laminin dependent motility and cell adhesion.

  14. The relationship between gene isoform multiplicity, number of exons and protein divergence.

    Directory of Open Access Journals (Sweden)

    Jordi Morata

    Full Text Available At present we know that phenotypic differences between organisms arise from a variety of sources, like protein sequence divergence, regulatory sequence divergence, alternative splicing, etc. However, we do not have yet a complete view of how these sources are related. Here we address this problem, studying the relationship between protein divergence and the ability of genes to express multiple isoforms. We used three genome-wide datasets of human-mouse orthologs to study the relationship between isoform multiplicity co-occurrence between orthologs (the fact that two orthologs have more than one isoform and protein divergence. In all cases our results showed that there was a monotonic dependence between these two properties. We could explain this relationship in terms of a more fundamental one, between exon number of the largest isoform and protein divergence. We found that this last relationship was present, although with variations, in other species (chimpanzee, cow, rat, chicken, zebrafish and fruit fly. In summary, we have identified a relationship between protein divergence and isoform multiplicity co-occurrence and explained its origin in terms of a simple gene-level property. Finally, we discuss the biological implications of these findings for our understanding of inter-species phenotypic differences.

  15. Quantification of various APP-mRNA isoforms and epistasis in Lesch-Nyhan disease.

    Science.gov (United States)

    Nguyen, Khue Vu; Nyhan, William L

    2017-03-16

    The present work is the development of a simple and specific kinetic method based on RT-PCR technique coupled with direct sequencing for quantification of various amyloid precursor protein-mRNA isoforms (APP-mRNA isoforms) in biological samples, especially for identifying the most abundant one that may decisive for the normal status or disease risk. Application of this kinetic method to the Lesch-Nyhan disease (LND) was performed and results indicated an epistasis between mutated hypoxanthine phosphoribosyltransferase1 (HPRT1) and APP genes. APP-mRNA isoform of 624bp, with a deletion starting after 49bp of the 5' end of exon 3 followed by a complete deletion of exons 4-15, mutations in exon 1: c.22C>T, p.L18F, and exon 3: c.269A>G, p.Q90R encoding APP207 isoform, was the most abundant one in most of the LND patients and would be responsible for the neurobehavioral syndrome in these patients. The method is useful for identifying the defective APP-mRNA isoform in LND patients, and in neurodevelopmental and neurodegenerative disorders in which the APP gene is involved in the pathogenesis of diseases such as autism, fragile X syndrome, amyotrophic lateral sclerosis, and Alzheimer's disease, and may pave the way for new strategies applicable to rational antisense drugs design. Copyright © 2017 Elsevier B.V. All rights reserved.

  16. Tau protein assembles into isoform- and disulfide-dependent polymorphic fibrils with distinct structural properties.

    Science.gov (United States)

    Furukawa, Yoshiaki; Kaneko, Kumi; Nukina, Nobuyuki

    2011-08-05

    Tauopathies are neurodegenerative diseases in which insoluble fibrillar aggregates of a microtubule-binding protein, Tau, are abnormally accumulated. Pathological Tau fibrils often exhibit structural polymorphisms that differ among phenotypically distinct tauopathies; however, a molecular mechanism to generate polymorphic Tau fibrils remains obscure. Here, we note the formation of a disulfide bond in isoforms of full-length Tau and show that the thiol-disulfide status as well as the isoform composition determines structural and morphological properties of Tau fibrils in vitro. Mainly two regions in a Tau primary sequence are found to act as structural blocks for building a protease-resistant core of Tau fibrils. Interactions among those two blocks for building a core structure depend upon the thiol-disulfide status in each isoform of Tau, which results in the formation of polymorphic fibrils with distinct structural properties. Furthermore, we have found that more diverse structures of Tau fibrils emerge through a cross-seeded fibrillation between heterologous pairs of Tau isoforms. We thus propose that isoform- and disulfide-dependent combinatorial interactions among multiple regions in a Tau sequence endow Tau fibrils with various structures, i.e. polymorphism.

  17. Tau Protein Assembles into Isoform- and Disulfide-dependent Polymorphic Fibrils with Distinct Structural Properties*

    Science.gov (United States)

    Furukawa, Yoshiaki; Kaneko, Kumi; Nukina, Nobuyuki

    2011-01-01

    Tauopathies are neurodegenerative diseases in which insoluble fibrillar aggregates of a microtubule-binding protein, Tau, are abnormally accumulated. Pathological Tau fibrils often exhibit structural polymorphisms that differ among phenotypically distinct tauopathies; however, a molecular mechanism to generate polymorphic Tau fibrils remains obscure. Here, we note the formation of a disulfide bond in isoforms of full-length Tau and show that the thiol-disulfide status as well as the isoform composition determines structural and morphological properties of Tau fibrils in vitro. Mainly two regions in a Tau primary sequence are found to act as structural blocks for building a protease-resistant core of Tau fibrils. Interactions among those two blocks for building a core structure depend upon the thiol-disulfide status in each isoform of Tau, which results in the formation of polymorphic fibrils with distinct structural properties. Furthermore, we have found that more diverse structures of Tau fibrils emerge through a cross-seeded fibrillation between heterologous pairs of Tau isoforms. We thus propose that isoform- and disulfide-dependent combinatorial interactions among multiple regions in a Tau sequence endow Tau fibrils with various structures, i.e. polymorphism. PMID:21659525

  18. Mammalian SEPT9 isoforms direct microtubule-dependent arrangements of septin core heteromers.

    Science.gov (United States)

    Sellin, Mikael E; Stenmark, Sonja; Gullberg, Martin

    2012-11-01

    Septin-family proteins assemble into rod-shaped heteromeric complexes that form higher-order arrangements at the cell cortex, where they serve apparently conserved functions as diffusion barriers and molecular scaffolds. There are 13 confirmed septin paralogues in mammals, which may be ubiquitous or tissue specific. Septin hetero-oligomerization appears homology subgroup directed, which in turn determines the subunit arrangement of six- to eight-subunit core heteromers. Here we address functional properties of human SEPT9, which, due to variable mRNA splicing, exists as multiple isoforms that differ between tissues. Myeloid K562 cells express three SEPT9 isoforms, all of which have an equal propensity to hetero-oligomerize with SEPT7-containing hexamers to generate octameric heteromers. However, due to limiting amounts of SEPT9, K562 cells contain both hexameric and octameric heteromers. To generate cell lines with controllable hexamer-to-octamer ratios and that express single SEPT9 isoforms, we developed a gene product replacement strategy. By this means we identified SEPT9 isoform-specific properties that either facilitate septin heteromer polymerization along microtubules or modulate the size range of submembranous septin disks-a prevalent septin structure in nonadhered cells. Our findings show that the SEPT9 expression level directs the hexamer-to-octamer ratio, and that the isoform composition and expression level together determine higher-order arrangements of septins.

  19. A subtle alternative splicing event gives rise to a widely expressed human RNase k isoform.

    Directory of Open Access Journals (Sweden)

    Evangelos D Karousis

    Full Text Available Subtle alternative splicing leads to the formation of RNA variants lacking or including a small number of nucleotides. To date, the impact of subtle alternative splicing phenomena on protein biosynthesis has been studied in frame-preserving incidents. On the contrary, mRNA isoforms derived from frame-shifting events were poorly studied and generally characterized as non-coding. This work provides evidence for a frame-shifting subtle alternative splicing event which results in the production of a novel protein isoform. We applied a combined molecular approach for the cloning and expression analysis of a human RNase κ transcript (RNase κ-02 which lacks four consecutive bases compared to the previously isolated RNase κ isoform. RNase κ-02 mRNA is expressed in all human cell lines tested end encodes the synthesis of a 134-amino-acid protein by utilizing an alternative initiation codon. The expression of RNase κ-02 in the cytoplasm of human cells was verified by Western blot and immunofluorescence analysis using a specific polyclonal antibody developed on the basis of the amino-acid sequence difference between the two protein isoforms. The results presented here show that subtle changes during mRNA splicing can lead to the expression of significantly altered protein isoforms.

  20. Novel frataxin isoforms may contribute to the pathological mechanism of Friedreich ataxia.

    Directory of Open Access Journals (Sweden)

    Haiyan Xia

    Full Text Available Friedreich ataxia (FRDA is an inherited neurodegenerative disease caused by frataxin (FXN deficiency. The nervous system and heart are the most severely affected tissues. However, highly mitochondria-dependent tissues, such as kidney and liver, are not obviously affected, although the abundance of FXN is normally high in these tissues. In this study we have revealed two novel FXN isoforms (II and III, which are specifically expressed in affected cerebellum and heart tissues, respectively, and are functional in vitro and in vivo. Increasing the abundance of the heart-specific isoform III significantly increased the mitochondrial aconitase activity, while over-expression of the cerebellum-specific isoform II protected against oxidative damage of Fe-S cluster-containing aconitase. Further, we observed that the protein level of isoform III decreased in FRDA patient heart, while the mRNA level of isoform II decreased more in FRDA patient cerebellum compared to total FXN mRNA. Our novel findings are highly relevant to understanding the mechanism of tissue-specific pathology in FRDA.