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Sample records for isoelectric point iep

  1. Morphology and the isoelectric point of nanosized aqueous ceria sols

    International Nuclear Information System (INIS)

    Gulicovski, Jelena J.; Bračko, Ines; Milonjić, Slobodan K.

    2014-01-01

    Ceria sols were synthesized by a forced hydrolysis method using cerium sulphate or cerium nitrate as precursors. High-resolution TEM was used to determine the microstructure and particle size of CeO 2 . A polycrystalline structure of ceria sols with crystallite sizes of 4.4 and 8.6 nm was obtained. The particles had a well-defined polyhedral shape. Direct bandgaps for ceria nanoparticles were 2.80 and 3.31 eV, respectively. The isoelectric point, pH iep , of ceria sols in KNO 3 solutions was investigated. The isoelectric point of ceria was found to be pH iep  = 7.6 ± 0.2. The obtained results indicate that the isoelectric point in the presence of SO 4 2− ions shifts to lower pH value. The particle size of ceria increases with pH, reaching the maximum value at pH iep as the result of agglomeration . - Highlights: • Ceria sols with cubic phase nanoparticles have been successfully synthesized. • The isoelectric point of ceria oxide in KNO 3 solutions was p Hiep  = 7.6 ± 0.2. • The specific adsorption of SO 4 2− ions causes a shift in IEP to lower pH (pH iep  = 6.7). • The correlation between IEP and the maximum diameter of particles was observed

  2. Morphology and the isoelectric point of nanosized aqueous ceria sols

    Energy Technology Data Exchange (ETDEWEB)

    Gulicovski, Jelena J. [The Vinča Institute of Nuclear Sciences, University of Belgrade, POB 522, 11001 Belgrade (Serbia); Bračko, Ines [Advanced Materials Department, Jozef Stefan Institute, Ljubljana (Slovenia); Milonjić, Slobodan K., E-mail: smiloni@vinca.rs [The Vinča Institute of Nuclear Sciences, University of Belgrade, POB 522, 11001 Belgrade (Serbia)

    2014-12-15

    Ceria sols were synthesized by a forced hydrolysis method using cerium sulphate or cerium nitrate as precursors. High-resolution TEM was used to determine the microstructure and particle size of CeO{sub 2}. A polycrystalline structure of ceria sols with crystallite sizes of 4.4 and 8.6 nm was obtained. The particles had a well-defined polyhedral shape. Direct bandgaps for ceria nanoparticles were 2.80 and 3.31 eV, respectively. The isoelectric point, pH{sub iep}, of ceria sols in KNO{sub 3} solutions was investigated. The isoelectric point of ceria was found to be pH{sub iep} = 7.6 ± 0.2. The obtained results indicate that the isoelectric point in the presence of SO{sub 4}{sup 2−} ions shifts to lower pH value. The particle size of ceria increases with pH, reaching the maximum value at pH{sub iep} as the result of agglomeration{sub .} - Highlights: • Ceria sols with cubic phase nanoparticles have been successfully synthesized. • The isoelectric point of ceria oxide in KNO{sub 3} solutions was p{sub Hiep} = 7.6 ± 0.2. • The specific adsorption of SO{sub 4}{sup 2−} ions causes a shift in IEP to lower pH (pH{sub iep} = 6.7). • The correlation between IEP and the maximum diameter of particles was observed.

  3. Lipoprotein lipase isoelectric point isoforms in humans

    DEFF Research Database (Denmark)

    Badia-Villanueva, M.; Carulla, P.; Carrascal, M.

    2014-01-01

    -heparin plasma (PHP), LPL consists of a pattern of more than 8 forms of the same apparent molecular weight, but different isoelectric point (pI). In the present study we describe, for the first time, the existence of at least nine LPL pI isoforms in human PHP, with apparent pI between 6.8 and 8.6. Separation...

  4. Effect of particle size on colloidal zirconia rheology at the isoelectric point

    International Nuclear Information System (INIS)

    Leong, Y.K.; Scales, P.J.; Healy, T.W.; Boger, D.V.

    1995-01-01

    This paper examines the effects of particle concentration and size on the yield stress of ZrO 2 suspensions at a well-defined surface chemistry condition of the isoelectric point (IEP). At the IEP, the relationship between yield stress τ y max and particulate volume fraction φ s , and mean particle size d was evaluated to be τ y max = K φ s 4.0 /d 2.0 . The difference in size distribution of the various ZrO 2 suspensions examined causes some degree of scatter in the data used to establish the τ y max , φ s , and d relation. The use of particle concentration n t based on the fine size fraction instead of volume fraction φ s provided a better correlation, because the fine particles govern the properties of the flocculated network structure

  5. Using Gel Electrophoresis To Illustrate Protein Diversity and Isoelectric Point.

    Science.gov (United States)

    Browning, Mark; Vanable, Joseph

    2002-01-01

    Demonstrates the differences in protein structures by focusing on isoelectric point with an experiment that is observable under certain pH levels in gel electrophoresis. Explains the electrophoresis procedure and reports results of the experiments. (YDS)

  6. Prediction of Isoelectric Point of Manganese and Cobalt Lamellar Oxides: Application to Controlled Synthesis of Mixed Oxides.

    Science.gov (United States)

    Tang, Céline; Giaume, Domitille; Guerlou-Demourgues, Liliane; Lefèvre, Grégory; Barboux, Philippe

    2018-05-30

    To design novel layered materials, bottom-up strategy is very promising. It consists of (1) synthesizing various layered oxides, (2) exfoliating them, then (3) restacking them in a controlled way. The last step is based on electrostatic interactions between different layered oxides and is difficult to control. The aim of this study is to facilitate this step by predicting the isoelectric point (IEP) of exfoliated materials. The Multisite Complexation model (MUSIC) was used for this objective and was shown to be able to predict IEP from the mean oxidation state of the metal in the (hydr)oxides, as the main parameter. Moreover, the effect of exfoliation on IEP has also been calculated. Starting from platelets with a high basal surface area over total surface area, we show that the exfoliation process has no impact on calculated IEP value, as verified with experiments. Moreover, the restacked materials containing different monometallic (hydr)oxide layers also have an IEP consistent with values calculated with the model. This study proves that MUSIC model is a useful tool to predict IEP of various complex metal oxides and hydroxides.

  7. Nanoscale roughness and morphology affect the IsoElectric Point of titania surfaces.

    Directory of Open Access Journals (Sweden)

    Francesca Borghi

    Full Text Available We report on the systematic investigation of the role of surface nanoscale roughness and morphology on the charging behaviour of nanostructured titania (TiO2 surfaces in aqueous solutions. IsoElectric Points (IEPs of surfaces have been characterized by direct measurement of the electrostatic double layer interactions between titania surfaces and the micrometer-sized spherical silica probe of an atomic force microscope in NaCl aqueous electrolyte. The use of a colloidal probe provides well-defined interaction geometry and allows effectively probing the overall effect of nanoscale morphology. By using supersonic cluster beam deposition to fabricate nanostructured titania films, we achieved a quantitative control over the surface morphological parameters. We performed a systematical exploration of the electrical double layer properties in different interaction regimes characterized by different ratios of characteristic nanometric lengths of the system: the surface rms roughness Rq, the correlation length ξ and the Debye length λD. We observed a remarkable reduction by several pH units of IEP on rough nanostructured surfaces, with respect to flat crystalline rutile TiO2. In order to explain the observed behavior of IEP, we consider the roughness-induced self-overlap of the electrical double layers as a potential source of deviation from the trend expected for flat surfaces.

  8. The differences in the isoelectric points of biofilm-positive and biofilm-negative Candida parapsilosis strains.

    Science.gov (United States)

    Ruzicka, Filip; Horka, Marie; Hola, Veronika; Kubesova, Anna; Pavlik, Tomas; Votava, Miroslav

    2010-03-01

    The isoelectric points of 39 Candida parapsilosis strains were determined by means of capillary isoelectric focusing. The value of the isoelectric point corresponded well with cell surface hydrophobicity, as well as with the ability to form biofilm in these yeasts. Copyright 2010 Elsevier B.V. All rights reserved.

  9. Effect of cations in the background electrolyte on the adsorption kinetics of copper and cadmium and the isoelectric point of imogolite

    International Nuclear Information System (INIS)

    Arancibia-Miranda, Nicolás; Silva-Yumi, Jorge; Escudey, Mauricio

    2015-01-01

    Highlights: • Effect of various cations on the IEP of imogolite was studied. • Studied adsorption kinetics of Cd and Cu on imogolite in the presence of cations. • K"+ acted as an indifferent electrolyte and did not affect the IEP of imogolite. • Adsorption in the presence of K"+ is described well by three of the four models. • These include pseudo-second order, Elovich equation, and Weber–Morris model. - Abstract: Modification of surface charge and changes in the isoelectric point (IEP) of synthetic imogolite were studied for various cations in the background electrolyte (K"+, NH_4"+, Mg"2"+, and Ca"2"+). From the electrophoretic mobility data, it was established that the K"+ (KCl) concentration does not affect the IEP of imogolite; therefore, KCl is a suitable background electrolyte. In terms of the magnitude of changes in the IEP and surface charge, the cations may be ranked in the following order: Mg"2"+ ≈ Ca"2"+ >> NH_4"+ >> K"+. Four different kinetic models were used to evaluate the influence of Mg"2"+, Ca"2"+, NH_4"+, and K"+ on the adsorption of Cd and Cu on synthetic imogolite. When adsorption occurs in the presence of cations with the exception of K"+, the kinetics of the process is well described by the pseudo-first order model. On the other hand, when adsorption is conducted in the presence of K"+, the adsorption kinetics is well described by the pseudo-second order, Elovich, and Weber–Morris models. From the surface charge measurements, the affinity between imogolite and the cations and their effect on the adsorption of trace elements, namely Cu and Cd, were established.

  10. Effect of cations in the background electrolyte on the adsorption kinetics of copper and cadmium and the isoelectric point of imogolite

    Energy Technology Data Exchange (ETDEWEB)

    Arancibia-Miranda, Nicolás, E-mail: nicolas.arancibia@usach.cl [Center for the Development of Nanoscience and Nanotechnology, CEDENNA, 9170124, Santiago (Chile); Facultad de Química y Biología, Universidad de Santiago de Chile, Av. B. O' Higgins, 3363, Santiago (Chile); Silva-Yumi, Jorge [Center for the Development of Nanoscience and Nanotechnology, CEDENNA, 9170124, Santiago (Chile); Escudey, Mauricio [Center for the Development of Nanoscience and Nanotechnology, CEDENNA, 9170124, Santiago (Chile); Facultad de Química y Biología, Universidad de Santiago de Chile, Av. B. O' Higgins, 3363, Santiago (Chile)

    2015-12-15

    Highlights: • Effect of various cations on the IEP of imogolite was studied. • Studied adsorption kinetics of Cd and Cu on imogolite in the presence of cations. • K{sup +} acted as an indifferent electrolyte and did not affect the IEP of imogolite. • Adsorption in the presence of K{sup +} is described well by three of the four models. • These include pseudo-second order, Elovich equation, and Weber–Morris model. - Abstract: Modification of surface charge and changes in the isoelectric point (IEP) of synthetic imogolite were studied for various cations in the background electrolyte (K{sup +}, NH{sub 4}{sup +}, Mg{sup 2+}, and Ca{sup 2+}). From the electrophoretic mobility data, it was established that the K{sup +} (KCl) concentration does not affect the IEP of imogolite; therefore, KCl is a suitable background electrolyte. In terms of the magnitude of changes in the IEP and surface charge, the cations may be ranked in the following order: Mg{sup 2+} ≈ Ca{sup 2+} >> NH{sub 4}{sup +} >> K{sup +}. Four different kinetic models were used to evaluate the influence of Mg{sup 2+}, Ca{sup 2+}, NH{sub 4}{sup +}, and K{sup +} on the adsorption of Cd and Cu on synthetic imogolite. When adsorption occurs in the presence of cations with the exception of K{sup +}, the kinetics of the process is well described by the pseudo-first order model. On the other hand, when adsorption is conducted in the presence of K{sup +}, the adsorption kinetics is well described by the pseudo-second order, Elovich, and Weber–Morris models. From the surface charge measurements, the affinity between imogolite and the cations and their effect on the adsorption of trace elements, namely Cu and Cd, were established.

  11. Gradient-free determination of isoelectric points of proteins on chip.

    Science.gov (United States)

    Łapińska, Urszula; Saar, Kadi L; Yates, Emma V; Herling, Therese W; Müller, Thomas; Challa, Pavan K; Dobson, Christopher M; Knowles, Tuomas P J

    2017-08-30

    The isoelectric point (pI) of a protein is a key characteristic that influences its overall electrostatic behaviour. The majority of conventional methods for the determination of the isoelectric point of a molecule rely on the use of spatial gradients in pH, although significant practical challenges are associated with such techniques, notably the difficulty in generating a stable and well controlled pH gradient. Here, we introduce a gradient-free approach, exploiting a microfluidic platform which allows us to perform rapid pH change on chip and probe the electrophoretic mobility of species in a controlled field. In particular, in this approach, the pH of the electrolyte solution is modulated in time rather than in space, as in the case for conventional determinations of the isoelectric point. To demonstrate the general approachability of this platform, we have measured the isoelectric points of representative set of seven proteins, bovine serum albumin, β-lactoglobulin, ribonuclease A, ovalbumin, human transferrin, ubiquitin and myoglobin in microlitre sample volumes. The ability to conduct measurements in free solution thus provides the basis for the rapid determination of isoelectric points of proteins under a wide variety of solution conditions and in small volumes.

  12. Isoelectric Point, Electric Charge, and Nomenclature of the Acid-Base Residues of Proteins

    Science.gov (United States)

    Maldonado, Andres A.; Ribeiro, Joao M.; Sillero, Antonio

    2010-01-01

    The main object of this work is to present the pedagogical usefulness of the theoretical methods, developed in this laboratory, for the determination of the isoelectric point (pI) and the net electric charge of proteins together with some comments on the naming of the acid-base residues of proteins. (Contains 8 figures and 4 tables.)

  13. The mechanism of uranium adsorption on Resin 508 and isoelectric point of the resin

    International Nuclear Information System (INIS)

    Han Qingping; Lu Weichang; Su Huijuan; Hu Jinbo; Zhang Liqin; Chen Banglin

    1990-01-01

    The adsorption process of uranium by Resin 508 at the solid-liquid interface was investigated and the mechanism of uranium adsorption including adsorption dynamics, adsorption thermodynamics and isoelectric point of resin was studied. The results are as follows: The maximum of uranium adsorption is attained at pH5-7; Uranium adsorption isotherm by Resin 508 in experimental conditions agrees with Langmuir's adsorption isotherm, the maximum of uranium adsorbed (Vm) is 716 mg U/g-dried resin; The adsorption of uranium by Resin 508 is an endothermic reaction and ΔH = 16.87 kJ/mol; The exchange-adsorption rate is mainly controlled by liquid film diffusion; The isoelectric points of Resin 508 before and after uranium adsorption are found to be pH7.5 and pH5.7 respectively. It is a specific adsorption for uranium

  14. Multijunction Capillary Isoelectric Focusing Device Combined with Online Membrane-Assisted Buffer Exchanger Enables Isoelectric Point Fractionation of Intact Human Plasma Proteins for Biomarker Discovery.

    Science.gov (United States)

    Pirmoradian, Mohammad; Astorga-Wells, Juan; Zubarev, Roman A

    2015-12-01

    Prefractionation of proteins is often employed to improve analysis specificity in proteomics. Prefractionation based on the isoelectric point (pI) is particularly attractive because pI is a well-defined parameter and it is orthogonal to hydrophobicity on which reversed-phase chromatography is based. However, direct capillary electrophoresis of blood proteins is challenging due to its high content of salts and charged small molecules. Here, we couple an online desalinator device to our multijunction capillary isoelectric focusing (MJ-CIEF) instrument and perform direct isoelectric separation of human blood plasma. In a proof-of-principle experiment, pooled samples of patients with progressive mild cognitive impairment and corresponding healthy controls were investigated. Injection of 3 μL of plasma containing over 100 μg of proteins into the desalinator was followed by pI fractionation with MJ-CIEF in less than 1 h. Shotgun proteomics of 12 collected fractions from each of the 5 replicates of pooled samples resulted in the identification and accurate quantification (median CV between the replicates is <4%) of nearly 365 protein groups from 4030 unique peptides (with <1% FDR for both peptides and proteins). The obtained results include several proteins previously reported as AD markers. The isoelectric point of each quantified protein was calculated using a set of 7 synthetic peptides spiked into the samples. Several proteins with a significant pI shift between their isoforms in the patient and control samples were identified. The presented method is straightforward, robust, and scalable; therefore, it can be used in both biological and clinical applications.

  15. Study of the solubility of a modified Bacillus licheniformis alpha-amylase around the isoelectric point

    DEFF Research Database (Denmark)

    Faber, Cornilius; Hobley, Timothy John; Mollerup, Jørgen

    2007-01-01

    The solubility of a modified recombinant Bacillus licheniformis alpha-amylase (mBLA) has been studied by batch crystallization. A semi-pure preparation was chosen containing five isoforms with pI values from 6 to 7.3 (weighted average of 6.6). Small amounts (... sodium sulfate at all pH values and increased with 0.5 mol.L-1 sodium thiocyanate at pH 7 and pH 8. The effect of anions on alpha-amylase solubility followed the Hofmeister series, and only weak evidence of reversal was seen below the isoelectric point. Cations had little effect on solubility. The sign...... and magnitude of the alpha-amylase zeta potential was determined in the presence and absence of 0.1 mol.L-1 salt. Qualitatively, zeta potential correctly predicted the different salts influence on mBLA solubility....

  16. Monte Carlo simulations of flexible polyanions complexing with whey proteins at their isoelectric point.

    Science.gov (United States)

    de Vries, R

    2004-02-15

    Electrostatic complexation of flexible polyanions with the whey proteins alpha-lactalbumin and beta-lactoglobulin is studied using Monte Carlo simulations. The proteins are considered at their respective isoelectric points. Discrete charges on the model polyelectrolytes and proteins interact through Debye-Huckel potentials. Protein excluded volume is taken into account through a coarse-grained model of the protein shape. Consistent with experimental results, it is found that alpha-lactalbumin complexes much more strongly than beta-lactoglobulin. For alpha-lactalbumin, strong complexation is due to localized binding to a single large positive "charge patch," whereas for beta-lactoglobulin, weak complexation is due to diffuse binding to multiple smaller charge patches. Copyright 2004 American Institute of Physics

  17. IEP as a parameter characterizing the pH-dependent surface charging of materials other than metal oxides.

    Science.gov (United States)

    Kosmulski, Marek

    2012-01-01

    The numerical values of points of zero charge (PZC, obtained by potentiometric titration) and of isoelectric points (IEP) of various materials reported in the literature have been analyzed. In sets of results reported for the same chemical compound (corresponding to certain chemical formula and crystallographic structure), the IEP are relatively consistent. In contrast, in materials other than metal oxides, the sets of PZC are inconsistent. In view of the inconsistence in the sets of PZC and of the discrepancies between PZC and IEP reported for the same material, it seems that IEP is more suitable than PZC as the unique number characterizing the pH-dependent surface charging of materials other than metal oxides. The present approach is opposite to the usual approach, in which the PZC and IEP are considered as two equally important parameters characterizing the pH-dependent surface charging of materials other than metal oxides. Copyright © 2012 Elsevier B.V. All rights reserved.

  18. HPLC separation of human serum albumin isoforms based on their isoelectric points

    Science.gov (United States)

    Bonilla, Lucía; Torres, María José; Schopfer, Francisco; Freeman, Bruce A.; Armas, Larissa; Ricciardi, Alejandro; Alvarez, Beatriz; Radi, Rafael

    2014-01-01

    Human serum albumin (HSA) is the most abundant protein in plasma. Cys34, the only free Cys residue, is the predominant plasma thiol and a relevant sacrificial antioxidant. Both in vivo circulating HSA and pharmaceutical preparations are heterogeneous with respect to the oxidation state of Cys34. In this work, we developed an external pH gradient chromatofocusing procedure that allows the analysis of the oxidation status of HSA in human plasma and biopharmaceutical products based on the different apparent isoelectric points and chemical properties of the redox isoforms. Specifically, reduced-mercury blocked HSA (HSA–SHg+), HSA with Cys34 oxidized to sulfenic acid (HSA–SOH) and HSA oxidized to sulfinate anion (HSA–SO2−) can be separated with resolutions of 1.4 and 3.1 (first and last pair) and hence quantified and purified. In addition, an N-terminally degraded isoform (HSA3–585) in different redox states can be resolved as well. Confirmation of the identity of the chromatofocusing isolated isoforms was achieved by high resolution whole protein MS. It is proposed that the chromatofocusing procedure can be used to produce more exact and complete descriptions of the redox status of HSA in vivo and in vitro. Finally, the scalability capabilities of the chromatofocusing procedure allow for the preparation of highly pure standards of several redox isoforms of HSA PMID:24316526

  19. Measuring protein isoelectric points by AFM-based force spectroscopy using trace amounts of sample

    Science.gov (United States)

    Guo, Shifeng; Zhu, Xiaoying; Jańczewski, Dominik; Lee, Serina Siew Chen; He, Tao; Teo, Serena Lay Ming; Vancso, G. Julius

    2016-09-01

    Protein charge at various pH and isoelectric point (pI) values is important in understanding protein function. However, often only trace amounts of unknown proteins are available and pI measurements cannot be obtained using conventional methods. Here, we show a method based on the atomic force microscope (AFM) to determine pI using minute quantities of proteins. The protein of interest is immobilized on AFM colloidal probes and the adhesion force of the protein is measured against a positively and a negatively charged substrate made by layer-by-layer deposition of polyelectrolytes. From the AFM force-distance curves, pI values with an estimated accuracy of ±0.25 were obtained for bovine serum albumin, myoglobin, fibrinogen and ribonuclease A over a range of 4.7-9.8. Using this method, we show that the pI of the ‘footprint’ of the temporary adhesive proteins secreted by the barnacle cyprid larvae of Amphibalanus amphitrite is in the range 9.6-9.7.

  20. Electroacoustic isoelectric point determinations of bauxite refinery residues: different neutralization techniques and minor mineral effects.

    Science.gov (United States)

    Freire, Tiago S S; Clark, Malcolm W; Comarmond, M Josick; Payne, Timothy E; Reichelt-Brushett, Amanda J; Thorogood, Gordon J

    2012-08-14

    Bauxite refinery residue (BRR) is a highly caustic, iron hydroxide-rich byproduct from alumina production. Some chemical treatments of BRR reduce soluble alkalinity and lower residue pH (to values work shows that minor mineral components in complex mineral systems may have a disproportionate effect on the observable bulk IEP. Furthermore, this work shows the appropriateness of electroacoustic techniques in investigating samples with significant soluble mineral components (e.g., ANC).

  1. Binding affinity and adhesion force of organophosphate hydrolase enzyme with soil particles related to the isoelectric point of the enzyme.

    Science.gov (United States)

    Islam, Shah Md Asraful; Yeasmin, Shabina; Islam, Md Saiful; Islam, Md Shariful

    2017-07-01

    The binding affinity of organophosphate hydrolase enzyme (OphB) with soil particles in relation to the isoelectric point (pI) was studied. Immobilization of OphB with soil particles was observed by confocal microscopy, Fourier transform infrared spectroscopy (FT-IR), and Atomic force microscopy (AFM). The calculated pI of OphB enzyme was increased from 8.69 to 8.89, 9.04 and 9.16 by the single, double and triple mutant of OphB enzyme, respectively through the replacement of negatively charged aspartate with positively charged histidine. Practically, the binding affinity was increased to 5.30%, 11.50%, and 16.80% for single, double and triple mutants, respectively. In contrast, enzyme activity of OphB did not change by the mutation of the enzyme. On the other hand, adhesion forces were gradually increased for wild type OphB enzyme (90 pN) to 96, 100 and 104 pN for single, double and triple mutants of OphB enzyme, respectively. There was an increasing trend of binding affinity and adhesion force by the increase of isoelectric point (pI) of OphB enzyme. Copyright © 2017 Elsevier Inc. All rights reserved.

  2. Purification, enzymatic characterization, and nucleotide sequence of a high-isoelectric-point alpha-glucosidase from barley malt

    DEFF Research Database (Denmark)

    Frandsen, T P; Lok, F; Mirgorodskaya, E

    2000-01-01

    in the transition state complex. Mass spectrometry of tryptic fragments assigned the 92-kD protein to a barley cDNA (GenBank accession no. U22450) that appears to encode an alpha-glucosidase. A corresponding sequence (HvAgl97; GenBank accession no. AF118226) was isolated from a genomic phage library using a c......High-isoelectric-point (pI) alpha-glucosidase was purified 7, 300-fold from an extract of barley (Hordeum vulgare) malt by ammonium sulfate fractionation, ion-exchange, and butyl-Sepharose chromatography. The enzyme had high activity toward maltose (k(cat) = 25 s(-1)), with an optimum at pH 4...

  3. A lower isoelectric point increases signal sequence-mediated secretion of recombinant proteins through a bacterial ABC transporter.

    Science.gov (United States)

    Byun, Hyunjong; Park, Jiyeon; Kim, Sun Chang; Ahn, Jung Hoon

    2017-12-01

    Efficient protein production for industrial and academic purposes often involves engineering microorganisms to produce and secrete target proteins into the culture. Pseudomonas fluorescens has a TliDEF ATP-binding cassette transporter, a type I secretion system, which recognizes C-terminal LARD3 signal sequence of thermostable lipase TliA. Many proteins are secreted by TliDEF in vivo when recombined with LARD3, but there are still others that cannot be secreted by TliDEF even when LARD3 is attached. However, the factors that determine whether or not a recombinant protein can be secreted through TliDEF are still unknown. Here, we recombined LARD3 with several proteins and examined their secretion through TliDEF. We found that the proteins secreted via LARD3 are highly negatively charged with highly-acidic isoelectric points (pI) lower than 5.5. Attaching oligo-aspartate to lower the pI of negatively-charged recombinant proteins improved their secretion, and attaching oligo-arginine to negatively-charged proteins blocked their secretion by LARD3. In addition, negatively supercharged green fluorescent protein (GFP) showed improved secretion, whereas positively supercharged GFP did not secrete. These results disclosed that proteins' acidic pI and net negative charge are major factors that determine their secretion through TliDEF. Homology modeling for TliDEF revealed that TliD dimer forms evolutionarily-conserved positively-charged clusters in its pore and substrate entrance site, which also partially explains the pI dependence of the TliDEF-dependent secretions. In conclusion, lowering the isoelectric point improved LARD3-mediated protein secretion, both widening the range of protein targets for efficient production via secretion and signifying an important aspect of ABC transporter-mediated secretions. © 2017 by The American Society for Biochemistry and Molecular Biology, Inc.

  4. Determination of the osmotic second virial coefficient and the demerization of beta-lactoglobulin in aqueous solutions with added salt at the isoelectric point

    NARCIS (Netherlands)

    Schaink, H.M.; Smit, J.A.M.

    2000-01-01

    Aqueous solutions of β-lactoglobulin (at the isoelectric point pH=5.18) have been studied by membrane osmometry. The osmotic second virial coefficient as well as the monomer–dimer equilibrium of β-lactoglobulin have been found to depend significantly on the salt concentration. At low salt

  5. The pH dependent surface charging and points of zero charge. VII. Update.

    Science.gov (United States)

    Kosmulski, Marek

    2018-01-01

    The pristine points of zero charge (PZC) and isoelectric points (IEP) of metal oxides and IEP of other materials from the recent literature, and a few older results (overlooked in previous searches) are summarized. This study is an update of the previous compilations by the same author [Surface Charging and Points of Zero Charge, CRC, Boca Raton, 2009; J. Colloid Interface Sci. 337 (2009) 439; 353 (2011) 1; 426 (2014) 209]. The field has been very active, but most PZC and IEP are reported for materials, which are very well-documented already (silica, alumina, titania, iron oxides). IEP of (nominally) Gd 2 O 3 , NaTaO 3 , and SrTiO 3 have been reported in the recent literature. Their IEP were not reported in older studies. Copyright © 2017 Elsevier B.V. All rights reserved.

  6. Design of a Software for Calculating Isoelectric Point of a Polypeptide According to Their Net Charge Using the Graphical Programming Language LabVIEW

    Science.gov (United States)

    Tovar, Glomen

    2018-01-01

    A software to calculate the net charge and to predict the isoelectric point (pI) of a polypeptide is developed in this work using the graphical programming language LabVIEW. Through this instrument the net charges of the ionizable residues of the chains of the proteins are calculated at different pH values, tabulated, pI is predicted and an Excel…

  7. Shift in the isoelectric-point of milk proteins as a consequence of adaptive divergence between the milks of mammalian species.

    LENUS (Irish Health Repository)

    Khaldi, Nora

    2011-07-29

    Abstract Background Milk proteins are required to proceed through a variety of conditions of radically varying pH, which are not identical across mammalian digestive systems. We wished to investigate if the shifts in these requirements have resulted in marked changes in the isoelectric point and charge of milk proteins during evolution. Results We investigated nine major milk proteins in 13 mammals. In comparison with a group of orthologous non-milk proteins, we found that 3 proteins κ-casein, lactadherin, and muc1 have undergone the highest change in isoelectric point during evolution. The pattern of non-synonymous substitutions indicate that selection has played a role in the isoelectric point shift, since residues that show significant evidence of positive selection are much more likely to be charged (p = 0.03 for κ-casein; p < 10-8 for muc1). However, this selection does not appear to be solely due to adaptation to the diversity of mammalian digestive systems, since striking changes are seen among species that resemble each other in terms of their digestion. Conclusion The changes in charge are most likely due to changes of other protein functions, rather than an adaptation to the different mammalian digestive systems. These functions may include differences in bioactive peptide releases in the gut between different mammals, which are known to be a major contributing factor in the functional and nutritional value of mammalian milk. This raises the question of whether bovine milk is optimal in terms of particular protein functions, for human nutrition and possibly disease resistance. This article was reviewed by Fyodor Kondrashov, David Liberles (nominated by David Ardell), and Christophe Lefevre (nominated by Mark Ragan).

  8. Isoelectric point-based fractionation by HiRIEF coupled to LC-MS allows for in-depth quantitative analysis of the phosphoproteome.

    Science.gov (United States)

    Panizza, Elena; Branca, Rui M M; Oliviusson, Peter; Orre, Lukas M; Lehtiö, Janne

    2017-07-03

    Protein phosphorylation is involved in the regulation of most eukaryotic cells functions and mass spectrometry-based analysis has made major contributions to our understanding of this regulation. However, low abundance of phosphorylated species presents a major challenge in achieving comprehensive phosphoproteome coverage and robust quantification. In this study, we developed a workflow employing titanium dioxide phospho-enrichment coupled with isobaric labeling by Tandem Mass Tags (TMT) and high-resolution isoelectric focusing (HiRIEF) fractionation to perform in-depth quantitative phosphoproteomics starting with a low sample quantity. To benchmark the workflow, we analyzed HeLa cells upon pervanadate treatment or cell cycle arrest in mitosis. Analyzing 300 µg of peptides per sample, we identified 22,712 phosphorylation sites, of which 19,075 were localized with high confidence and 1,203 are phosphorylated tyrosine residues, representing 6.3% of all detected phospho-sites. HiRIEF fractions with the most acidic isoelectric points are enriched in multiply phosphorylated peptides, which represent 18% of all the phospho-peptides detected in the pH range 2.5-3.7. Cross-referencing with the PhosphoSitePlus database reveals 1,264 phosphorylation sites that have not been previously reported and kinase association analysis suggests that a subset of these may be functional during the mitotic phase.

  9. The surface chemistry of sapphire-c: A literature review and a study on various factors influencing its IEP.

    Science.gov (United States)

    Lützenkirchen, J; Franks, G V; Plaschke, M; Zimmermann, R; Heberling, F; Abdelmonem, A; Darbha, G K; Schild, D; Filby, A; Eng, P; Catalano, J G; Rosenqvist, J; Preocanin, T; Aytug, T; Zhang, D; Gan, Y; Braunschweig, B

    2018-01-01

    A wide range of isoelectric points (IEPs) has been reported in the literature for sapphire-c (α-alumina), also referred to as basal plane, (001) or (0001), single crystals. Interestingly, the available data suggest that the variation of IEPs is comparable to the range of IEPs encountered for particles, although single crystals should be much better defined in terms of surface structure. One explanation for the range of IEPs might be the obvious danger of contaminating the small surface areas of single crystal samples while exposing them to comparatively large solution reservoirs. Literature suggests that factors like origin of the sample, sample treatment or the method of investigation all have an influence on the surfaces and it is difficult to clearly separate the respective, individual effects. In the present study, we investigate cause-effect relationships to better understand the individual effects. The reference IEP of our samples is between 4 and 4.5. High temperature treatment tends to decrease the IEP of sapphire-c as does UV treatment. Increasing the initial miscut (i.e. the divergence from the expected orientation of the crystal) tends to increase the IEP as does plasma cleaning, which can be understood assuming that the surfaces have become less hydrophobic due to the presence of more and/or larger steps with increasing miscut or due to amorphisation of the surface caused by plasma cleaning. Pre-treatment at very high pH caused an increase in the IEP. Surface treatments that led to IEPs different from the stable value of reference samples typically resulted in surfaces that were strongly affected by subsequent exposure to water. The streaming potential data appear to relax to the reference sample behavior after a period of time of water exposure. Combination of the zeta-potential measurements with AFM investigations support the idea that atomically smooth surfaces exhibit lower IEPs, while rougher surfaces (roughness on the order of nanometers) result

  10. Use of a Spreadsheet to Calculate the Net Charge of Peptides and Proteins as a Function of pH: An Alternative to Using "Canned" Programs to Estimate the Isoelectric Point of These Important Biomolecules

    Science.gov (United States)

    Sims, Paul A.

    2010-01-01

    An approach is presented that utilizes a spreadsheet to allow students to explore different means of calculating and visualizing how the charge on peptides and proteins varies as a function of pH. In particular, the concept of isoelectric point is developed to allow students to compare the results of their spreadsheet calculations with those of…

  11. Red de iep

    NARCIS (Netherlands)

    Hiemstra, J.A.; Kopinga, J.; Buiteveld, J.

    2007-01-01

    Het gebruik van de iep - ook resistente iepen - is in de afgelopen decennia dan ook sterk afgenomen. Hierdoor verdwijnt de iep steeds meer uit het Nederlandse landschap. De onvrede van groenbeheerders en boomkwekers leidde in de afgelopen jaren tot een aantal discussiebijeenkomsten over de toekomst

  12. Design of a software for calculating isoelectric point of a polypeptide according to their net charge using the graphical programming language LabVIEW.

    Science.gov (United States)

    Tovar, Glomen

    2018-01-01

    A software to calculate the net charge and to predict the isoelectric point (pI) of a polypeptide is developed in this work using the graphical programming language LabVIEW. Through this instrument the net charges of the ionizable residues of the polypeptide chains of the proteins are calculated at different pH values, tabulated, pI is predicted and an Excel (-xls) type file is generated. In this work, the experimental values of the pIs (pI) of different proteins are compared with the values of the pIs (pI) calculated graphically, achieving a correlation coefficient (R) of 0.934746 which represents a good reliability for a p program can constitute an instrument applicable in the laboratory, facilitating the calculation to graduate students and junior researchers. © 2017 by The International Union of Biochemistry and Molecular Biology, 46(1):39-46, 2018. © 2017 The International Union of Biochemistry and Molecular Biology.

  13. Alternative splicing studies of the reactive oxygen species gene network in Populus reveal two isoforms of high-isoelectric-point superoxide dismutase.

    Science.gov (United States)

    Srivastava, Vaibhav; Srivastava, Manoj Kumar; Chibani, Kamel; Nilsson, Robert; Rouhier, Nicolas; Melzer, Michael; Wingsle, Gunnar

    2009-04-01

    Recent evidence has shown that alternative splicing (AS) is widely involved in the regulation of gene expression, substantially extending the diversity of numerous proteins. In this study, a subset of expressed sequence tags representing members of the reactive oxygen species gene network was selected from the PopulusDB database to investigate AS mechanisms in Populus. Examples of all known types of AS were detected, but intron retention was the most common. Interestingly, the closest Arabidopsis (Arabidopsis thaliana) homologs of half of the AS genes identified in Populus are not reportedly alternatively spliced. Two genes encoding the protein of most interest in our study (high-isoelectric-point superoxide dismutase [hipI-SOD]) have been found in black cottonwood (Populus trichocarpa), designated PthipI-SODC1 and PthipI-SODC2. Analysis of the expressed sequence tag libraries has indicated the presence of two transcripts of PthipI-SODC1 (hipI-SODC1b and hipI-SODC1s). Alignment of these sequences with the PthipI-SODC1 gene showed that hipI-SODC1b was 69 bp longer than hipI-SODC1s due to an AS event involving the use of an alternative donor splice site in the sixth intron. Transcript analysis showed that the splice variant hipI-SODC1b was differentially expressed, being clearly expressed in cambial and xylem, but not phloem, regions. In addition, immunolocalization and mass spectrometric data confirmed the presence of hipI-SOD proteins in vascular tissue. The functionalities of the spliced gene products were assessed by expressing recombinant hipI-SOD proteins and in vitro SOD activity assays.

  14. Alternative Splicing Studies of the Reactive Oxygen Species Gene Network in Populus Reveal Two Isoforms of High-Isoelectric-Point Superoxide Dismutase1[C][W

    Science.gov (United States)

    Srivastava, Vaibhav; Srivastava, Manoj Kumar; Chibani, Kamel; Nilsson, Robert; Rouhier, Nicolas; Melzer, Michael; Wingsle, Gunnar

    2009-01-01

    Recent evidence has shown that alternative splicing (AS) is widely involved in the regulation of gene expression, substantially extending the diversity of numerous proteins. In this study, a subset of expressed sequence tags representing members of the reactive oxygen species gene network was selected from the PopulusDB database to investigate AS mechanisms in Populus. Examples of all known types of AS were detected, but intron retention was the most common. Interestingly, the closest Arabidopsis (Arabidopsis thaliana) homologs of half of the AS genes identified in Populus are not reportedly alternatively spliced. Two genes encoding the protein of most interest in our study (high-isoelectric-point superoxide dismutase [hipI-SOD]) have been found in black cottonwood (Populus trichocarpa), designated PthipI-SODC1 and PthipI-SODC2. Analysis of the expressed sequence tag libraries has indicated the presence of two transcripts of PthipI-SODC1 (hipI-SODC1b and hipI-SODC1s). Alignment of these sequences with the PthipI-SODC1 gene showed that hipI-SODC1b was 69 bp longer than hipI-SODC1s due to an AS event involving the use of an alternative donor splice site in the sixth intron. Transcript analysis showed that the splice variant hipI-SODC1b was differentially expressed, being clearly expressed in cambial and xylem, but not phloem, regions. In addition, immunolocalization and mass spectrometric data confirmed the presence of hipI-SOD proteins in vascular tissue. The functionalities of the spliced gene products were assessed by expressing recombinant hipI-SOD proteins and in vitro SOD activity assays. PMID:19176719

  15. In-capillary enrichment, proteolysis and separation using capillary electrophoresis with discontinuous buffers: application on proteins with moderately acidic and basic isoelectric points.

    Science.gov (United States)

    Nesbitt, Chandra A; Yeung, Ken K-C

    2009-01-01

    Advances in mass spectrometry and capillary-format separation continue to improve the sensitivity of protein analysis. Of equal importance is the miniaturization of sample pretreatment such as enrichment and proteolysis. In a previous report (Nesbitt et al., Electrophoresis, 2008, 29, 466-474), nanoliter-volume protein enrichment, tryptic digestion, and partial separation was demonstrated in capillary electrophoresis followed by MALDI mass spectral analysis. A discontinuous buffer system, consisting of ammonium (pH 10) and acetate (pH 4), was used to create a pH junction inside the capillary, trapping a protein with a neutral isoelectric point, myoglobin (pI 7.2). Moreover, co-enrichment of myoglobin with trypsin led to an in-capillary digestion. In this paper, the ability of this discontinuous buffer system to perform similar in-capillary sample pretreatment on proteins with moderately acidic and basic pI was studied and reported. Lentil lectin (pI 8.6) and a multi-phosphorylated protein, beta-casein (pI 5.1), were selected as model proteins. In addition to the previously shown tryptic digestion, proteolysis with endoproteinase Asp-N was also performed. Digestion of these acidic and basic pI proteins produced a few peptides with extreme pI values lying outside the trapping range of the discontinuous buffer. An alteration in the peptide trapping procedure was made to accommodate these analytes. Offline MALDI mass spectral analysis confirmed the presence of the expected peptides. The presented miniaturized sample pretreatment methodology was proven to be applicable on proteins with a moderately wide range of pI. Flexibility in the choice of protease was also evident.

  16. Resistentie geeft Iep weer toekomst

    NARCIS (Netherlands)

    Hiemstra, J.A.

    2013-01-01

    De iep was lang één van de meest gekweekte boomsoorten in Nederland, totdat deze door problemen met de iepziekte bij veel afnemers een slechte naam kreeg. Onderzoek door PPO en Alterra laat nu echter zien dat de iep weer volop toekomst heeft. De nieuwe rassen die inmiddels op de markt zijn, hebben

  17. Desarrollando el IEP de su Hijo. Guia para Padres (Developing Your Child's IEP. A Parent's Guide).

    Science.gov (United States)

    Rebhorn, Theresa

    This Spanish language guide for parents of children with disabilities explains the basics of the special education process, especially parent participation developing the child's individualized education program (IEP). The first section reviews the IEP process, including what's involved, the IEP meeting, who attends the IEP meeting, the role of…

  18. 34 CFR 300.321 - IEP Team.

    Science.gov (United States)

    2010-07-01

    ... 34 Education 2 2010-07-01 2010-07-01 false IEP Team. 300.321 Section 300.321 Education Regulations... Placements Individualized Education Programs § 300.321 IEP Team. (a) General. The public agency must ensure that the IEP Team for each child with a disability includes— (1) The parents of the child; (2) Not less...

  19. De iep heeft weer toekomst

    NARCIS (Netherlands)

    Hiemstra, J.A.

    2013-01-01

    Door de iepziekte is de iep grotendeels uit het landschap verdwenen. Zonde, want het is voor veel moeilijke plekken de ideale boom. Onderzoek van PPO en Alterra laat nu echter zien dat er voldoende resistente iepen zijn om de boom weer volop te gaan gebruiken.

  20. Increasing Student Involvement in IEPs

    Science.gov (United States)

    Nolan-Spohn, Hannah

    2016-01-01

    In this article, the author proposes that students who led their Individual Education Program (IEP) meetings were better informed about their own disabilities, rights, and accommodations, and that the act of leading the meeting resulted in improved self-advocacy and self-confidence. Also, in understanding their list of accommodations and…

  1. Capillary isoelectric focusing-useful tool for detection and quantification of lactic acid bacteria in milk

    Czech Academy of Sciences Publication Activity Database

    Růžička, F.; Horká, Marie; Holá, V.; Mlynariková, K.; Dráb, V.

    2016-01-01

    Roč. 9, č. 12 (2016), s. 3251-3257 ISSN 1936-9751 R&D Projects: GA MZd(CZ) NV16-29916A Institutional support: RVO:68081715 Keywords : capillary isoelectric focusing * enterococcus * isoelectric point * lactic acid bacteria Subject RIV: CB - Analytical Chemistry, Separation Impact factor: 2.038, year: 2016

  2. Facilitated IEP Meetings. PHP-c90

    Science.gov (United States)

    PACER Center, 2004

    2004-01-01

    To help special education planning teams reach agreements, the Minnesota Department of Education and the Minnesota Special Education Mediation Service (MNSEMS) provide the option of facilitated IEP meetings. This option is available for IEP (Individualized Education Program), IIIP (Individual Interagency Intervention Plan), and IFSP (Individual…

  3. Six Tips for Successful IEP Meetings

    Science.gov (United States)

    Diliberto, Jennifer A.; Brewer, Denise

    2012-01-01

    Individuals with Disabilities Education Improvement Act (IDEIA, 2004) mandates that each student with a disability has an individualized education program (IEP). The IEP serves as the curriculum roadmap for special education services. In order to generate a clear roadmap, full team communication is necessary. The purpose of this paper is to…

  4. Weer volop toekomst voor de iep

    NARCIS (Netherlands)

    Hiemstra, J.A.

    2014-01-01

    De iep is eeuwenlang een van de beeldbepalende bomen geweest in het Nederlandse landschap en de Nederlandse steden. Door de opkomst van de iepziekte zijn in de loop van de vorige eeuw veel van deze Hollandse iepen verdwenen. Door de voortdurende problemen met de iepziekte kreeg de iep bij veel

  5. IEP Use by General and Special Education Teachers

    OpenAIRE

    Kathleen Rotter

    2014-01-01

    The role of general and special educators in implementing the Individualized Education Program (IEP) is critical. This study investigated the perceptions of those teachers on when they read the IEP, how they measure attainment of IEP goals and objectives, and the IEPs usefulness in instructional planning. Four hundred twenty-six teachers from suburban school districts in central New Jersey were respondents. Results ind...

  6. The inadequacy of Individual Educational Program (IEP) goals for high school students with word-level reading difficulties.

    Science.gov (United States)

    Catone, William V; Brady, Susan A

    2005-06-01

    This investigation analyzed goals from the Individual Educational Programs (IEPs) of 54 high school students with diagnosed reading disabilities in basic skills (decoding and/or word identification). Results showed that for 73% of the students, the IEPs written when they were in high school failed to specify any objectives regarding their acute difficulties with basic skills. IEPs from earlier points in the students' educations were also reviewed, as available. For 23 of the students, IEPs were present in the students' files for three time points: elementary school (ES), middle school (MS), and high school (HS). Another 20 students from the sample of 54 had IEPs available for two time points (HS and either MS or ES). Comparisons with the IEPs from younger years showed a pattern of decline from ES to MS to HS in the percentage of IEPs that commented on or set goals pertaining to weaknesses in decoding. These findings suggest that basic skills deficits that persist into the upper grade levels are not being sufficiently targeted for remediation, and help explain why older students frequently fail to resolve their reading problems.

  7. Individualized Education Plans (IEPs) (For Parents)

    Science.gov (United States)

    ... of specific school skills, such as reading or math, as well as more general developmental skills, such ... to update the goals and make sure the levels of service meet your child's needs. However, IEPs ...

  8. Examining the Quality of IEPs for Young Children with Autism

    OpenAIRE

    Ruble, Lisa A.; McGrew, John; Dalrymple, Nancy; Jung, Lee Ann

    2010-01-01

    The purpose of this study was to develop an Individual Education Program (IEP) evaluation tool based on Individuals with Disabilities Education Act (IDEA) requirements and National Research Council recommendations for children with autism; determine the tool’s reliability; test the tool on a pilot sample of IEPs of young children; and examine associations between IEP quality and school, teacher, and child characteristics. IEPs for 35 students with autism (Mage = 6.1 years; SD = 1.6) from 35 d...

  9. My IEP: A Student-Directed Individualized Education Program Model

    Science.gov (United States)

    Royer, David J.

    2017-01-01

    Students with disabilities need to be more involved in planning and presenting individualized education program (IEP) meetings, and teachers need an effective, efficient curriculum to teach students how. "My IEP" curriculum uses folding graphic organizers to teach students to self-direct IEP meetings, targeting self-advocacy and…

  10. 34 CFR 300.112 - Individualized education programs (IEP).

    Science.gov (United States)

    2010-07-01

    ... 34 Education 2 2010-07-01 2010-07-01 false Individualized education programs (IEP). 300.112 Section 300.112 Education Regulations of the Offices of the Department of Education (Continued) OFFICE OF... education programs (IEP). The State must ensure that an IEP, or an IFSP that meets the requirements of...

  11. IEP Use by General and Special Education Teachers

    Directory of Open Access Journals (Sweden)

    Kathleen Rotter

    2014-04-01

    Full Text Available The role of general and special educators in implementing the Individualized Education Program (IEP is critical. This study investigated the perceptions of those teachers on when they read the IEP, how they measure attainment of IEP goals and objectives, and the IEPs usefulness in instructional planning. Four hundred twenty-six teachers from suburban school districts in central New Jersey were respondents. Results indicated that the majority of general and special education teachers are reading their students’ IEPs in a fairly timely manner and that they find them moderately useful in planning instruction. Results also suggested that teachers relied heavily on grades to document attainment of IEP goals and that teachers felt that the IEPs themselves could be improved if they were shorter with student-specific, critical information that was more relevant to classroom instruction.

  12. Music in the IEP: Therapy/Education.

    Science.gov (United States)

    Alley, Jayne M.

    1979-01-01

    The article discusses the roles of music education and music therapy in special education, specifically with reference to the concept of the individualized education program (IEP) as mandated by the Education for All Handicapped Children Act (P.L. 94-142). Journal availability: National Association for Music Therapy, Inc., P.O. Box 610, Lawrence,…

  13. De iep is weer te vertrouwen

    NARCIS (Netherlands)

    Hiemstra, J.A.; Beintema, N.

    2012-01-01

    De iep is door de iepziekte grotendeels uit Europa verdwenen. Mede dankzij Wagenings onderzoek zijn er nu resistente rassen op de markt. Wat nog ontbreekt is het vertrouwen van beheerders om de karakteristieke boom weer aan te planten. ‘Iepen waren synoniem met ellende.’

  14. IEP Documentation for Effective Systematic Facilitation

    Science.gov (United States)

    Eng, Peng-Sim

    2015-01-01

    This paper discusses how the process for IEP documentation was used in a training program for a group of young inexperienced teachers and teaching aides to effectively address the educational needs of children with diverse disabilities. Teachers at Kianh Centre in Vietnam received explicit instructions for writing effective functional individual…

  15. IEP Meetings: Building Compassion and Conversation

    Science.gov (United States)

    Fialka, Janice; Fialka-Feldman, Emma

    2017-01-01

    Why are IEP meetings often uncomfortable, awkward, and stiff? Educators and families all have a common goal--to support the growth of a child. Yet educators often feel confused that families act like reluctant participants who are just fulfilling a requirement. And families often feel that they're viewed as intruders, whose insights and dreams for…

  16. Capillary isoelectric focusing--useful tool for detection of the biofilm formation in Staphylococcus epidermidis.

    Science.gov (United States)

    Ruzicka, Filip; Horka, Marie; Hola, Veronika; Votava, Miroslav

    2007-03-01

    The biofilm formation is an important factor of S. epidermidis virulence. Biofilm-positive strains might be clinically more important than biofilm-negative ones. Unlike biofilm-negative staphylococci, biofilm-positive staphylococci are surrounded with an extracellular polysaccharide substance. The presence of this substance on the surface can affect physico-chemical properties of the bacterial cell, including surface charge. 73 S. epidermidis strains were examined for the presence of ica operon, for the ability to form biofilm by Christensen test tube method and for the production of slime by Congo red agar method. Isoelectric points (pI) of these strains were determined by means of Capillary Isoelectric Focusing. The biofilm negative strains focused near pI value 2.3, while the pI values of the biofilm positive strains were near 2.6. Isoelectric point is a useful criterion for the differentiation between biofilm-positive and biofilm-negative S. epidermidis strains.

  17. 34 CFR 300.323 - When IEPs must be in effect.

    Science.gov (United States)

    2010-07-01

    ... disability within its jurisdiction, an IEP, as defined in § 300.320. (b) IEP or IFSP for children aged three...), the IEP Team must consider an IFSP that contains the IFSP content (including the natural environments... accordance with the IEP procedures under this part. The IFSP may serve as the IEP of the child, if using the...

  18. Free flow and capillary isoelectric focusing of bacteria from the tomatoes plant tissues

    Czech Academy of Sciences Publication Activity Database

    Horká, Marie; Horký, J.; Matoušková, H.; Šlais, Karel

    2009-01-01

    Roč. 1216, č. 6 (2009), s. 1019-1024 ISSN 0021-9673 R&D Projects: GA AV ČR IAAX00310701 Institutional research plan: CEZ:AV0Z40310501 Keywords : free flow and capillary IEF * isoelectric point of microbes * tomatoes plant tissue suspension Subject RIV: CB - Analytical Chemistry, Separation Impact factor: 4.101, year: 2009

  19. Student award for outstanding research winner in the Ph.D. category for the 2017 society for biomaterials annual meeting and exposition, april 5-8, 2017, Minneapolis, Minnesota: Characterization of protein interactions with molecularly imprinted hydrogels that possess engineered affinity for high isoelectric point biomarkers.

    Science.gov (United States)

    Clegg, John R; Zhong, Justin X; Irani, Afshan S; Gu, Joann; Spencer, David S; Peppas, Nicholas A

    2017-06-01

    Molecularly imprinted polymers (MIPs) with selective affinity for protein biomarkers could find extensive utility as environmentally robust, cost-efficient biomaterials for diagnostic and therapeutic applications. In order to develop recognitive, synthetic biomaterials for prohibitively expensive protein biomarkers, we have developed a molecular imprinting technique that utilizes structurally similar, analogue proteins. Hydrogel microparticles synthesized by molecular imprinting with trypsin, lysozyme, and cytochrome c possessed an increased affinity for alternate high isoelectric point biomarkers both in isolation and plasma-mimicking adsorption conditions. Imprinted and non-imprinted P(MAA-co-AAm-co-DEAEMA) microgels containing PMAO-PEGMA functionalized polycaprolactone nanoparticles were net-anionic, polydisperse, and irregularly shaped. MIPs and control non-imprinted polymers (NIPs) exhibited regions of Freundlich and BET isotherm adsorption behavior in a range of non-competitive protein solutions, where MIPs exhibited enhanced adsorption capacity in the Freundlich isotherm regions. In a competitive condition, imprinting with analogue templates (trypsin, lysozyme) increased the adsorption capacity of microgels for cytochrome c by 162% and 219%, respectively, as compared to a 122% increase provided by traditional bulk imprinting with cytochrome c. Our results suggest that molecular imprinting with analogue protein templates is a viable synthetic strategy for enhancing hydrogel-biomarker affinity and promoting specific protein adsorption behavior in biological fluids. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 1565-1574, 2017. © 2017 Wiley Periodicals, Inc.

  20. The Effectiveness of Alternative IEP Dispute Resolution Practices

    Science.gov (United States)

    Scanlon, David; Saenz, Lauren; Kelly, Michael P.

    2018-01-01

    Alternative Individualized Education Program (IEP) dispute resolution models should respond to limitations of current options. An experimental IEP dispute resolution program provides parents and schools with an evidence-supported neutral perspective on what is needed for free and appropriate education (FAPE) and least restrictive environment…

  1. Promoting IEP Participation: Effects of Interventions, Considerations for CLD Students

    Science.gov (United States)

    Griffin, Megan M.

    2011-01-01

    Various interventions have been developed to promote student individualized education program (IEP) participation. Although they are generally endorsed by educators and researchers, critics argue that interventions to promote self-determination and IEP participation may be counter to the values of culturally and linguistically diverse (CLD)…

  2. Examining the Quality of IEPs for Young Children with Autism

    Science.gov (United States)

    Ruble, Lisa A.; McGrew, John; Dalrymple, Nancy; Jung, Lee Ann

    2010-01-01

    The purpose of this study was to develop an Individual Education Program (IEP) evaluation tool based on Individuals with Disabilities Education Act (IDEA) requirements and National Research Council recommendations for children with autism; determine the tool's reliability; test the tool on a pilot sample of IEPs of young children; and examine…

  3. Investigation of Health Care Components in Transition IEPs

    Science.gov (United States)

    Repetto, Jeanne B.; Jaress, Jennifer; Lindsey, Jenna; Bae, Jungah

    2016-01-01

    The purpose of this study was to assess the inclusion of health care components in transition Individualized Education Programs (IEPs) for students diagnosed with Other Health Impaired (OHI). In this study, we analyzed 50 IEPs of students with an OHI diagnosis to establish whether there are health-related components or other common health care…

  4. Back to the Basics: Practical Tips for IEP Writing

    Science.gov (United States)

    Patti, Angela L.

    2016-01-01

    The individualized education program (IEP) is the foundation for the provision of special education services for a child with a disability. While special education teachers learn about IEP writing in their teacher preparation programs, it can still be difficult to translate this knowledge into practice. Therefore, when faced with the task of…

  5. How Individualised Are the Individualised Education Programmes (IEPs): An Analysis of the Contents and Quality of the IEPs Goals

    Science.gov (United States)

    Sanches-Ferreira, Manuela; Lopes-dos-Santos, Pedro; Alves, Sílvia; Santos, Miguel; Silveira-Maia, Mónica

    2013-01-01

    The Individualised Education Programme (IEP) is a fundamental document that describes all educational responses to the additional support needs of students, setting up the guideline for their learning and developmental experiences. Specifically, the IEP goals represent the personal destination translated into desirable behaviours and skills that…

  6. Crystallization of isoelectrically homogeneous cholera toxin

    International Nuclear Information System (INIS)

    Spangler, B.D.; Westbrook, E.M.

    1989-01-01

    Past difficulty in growing good crystals of cholera toxin has prevented the study of the crystal structure of this important protein. The authors have determined that failure of cholera toxin to crystallize well has been due to its heterogeneity. They have now succeeded in overcoming the problem by isolating a single isoelectric variant of this oligomeric protein (one A subunit and five B subunits). Cholera toxin purified by their procedure readily forms large single crystals. The crystal form has been described previously. They have recorded data from native crystals of cholera toxin to 3.0-angstrom resolution with our electronic area detectors. With these data, they have found the orientation of a 5-fold symmetry axis within these crystals, perpendicular to the screw dyad of the crystal. They are now determining the crystal structure of cholera toxin by a combination of multiple heavy-atom isomorphous replacement and density modification techniques, making use of rotational 5-fold averaging of the B subunits

  7. Making MEPA-IEP work: tools for professionals.

    Science.gov (United States)

    McRoy, Ruth; Mica, Maryanne; Freundlich, Madelyn; Kroll, Joe

    2007-01-01

    The Multiethnic Placement Act of 1994 and the Interethnic Adoption Provisions of 1996 (MEPA-IEP) require states to develop plans that "provide for the diligent recruitment of potential foster and adoptive families that reflect the ethnic and racial diversity of children in the state for whom foster and adoptive homes are needed." This paper explores the background of MEPA-IEP, describes the disparate outcomes for minority children in the child welfare system, and identifies agency challenges in finding permanent families for African American children. Tools are provided for successfully recruiting families while following MEPA-IEP and avoiding potentially discriminatory practices in placement decisionmaking.

  8. Preparative isoelectric focusing of microorganisms in cellulose-based separation medium and subsequent analysis by CIEF and MALDI-TOF MS

    Czech Academy of Sciences Publication Activity Database

    Horká, Marie; Šlais, Karel; Šalplachta, Jiří; Růžička, F.

    2017-01-01

    Roč. 990, OCT (2017), s. 185-193 ISSN 0003-2670 R&D Projects: GA ČR(CZ) GA16-03749S; GA MV(CZ) VI20172020069; GA MZd(CZ) NV16-29916A Institutional support: RVO:68081715 Keywords : preparative isoelectric focusing * colored microorganisms * isoelectric points * CIEF and MALDI-TOF MS Subject RIV: CB - Analytical Chemistry, Separation OBOR OECD: Analytical chemistry Impact factor: 4.950, year: 2016

  9. Comparison of polyacrylamide and agarose gel thin-layer isoelectric focusing for the characterization of beta-lactamases.

    Science.gov (United States)

    Vecoli, C; Prevost, F E; Ververis, J J; Medeiros, A A; O'Leary, G P

    1983-08-01

    Plasmid-mediated beta-lactamases from strains of Escherichia coli and Pseudomonas aeruginosa were separated by isoelectric focusing on a 0.8-mm thin-layer agarose gel with a pH gradient of 3.5 to 9.5. Their banding patterns and isoelectric points were compared with those obtained with a 2.0-mm polyacrylamide gel as the support medium. The agarose method produced banding patterns and isoelectric points which corresponded to the polyacrylamide gel data for most samples. Differences were observed for HMS-1 and PSE-1 beta-lactamases. The HMS-1 sample produced two highly resolvable enzyme bands in agarose gels rather than the single faint enzyme band observed on polyacrylamide gels. The PSE-1 sample showed an isoelectric point shift of 0.2 pH unit between polyacrylamide and agarose gel (pI 5.7 and 5.5, respectively). The short focusing time, lack of toxic hazard, and ease of formulation make agarose a practical medium for the characterization of beta-lactamases.

  10. Individualized Education Program (IEP) Facilitation. A Guide for Parents of Children & Youth (Ages 3-21)

    Science.gov (United States)

    Center for Appropriate Dispute Resolution in Special Education (CADRE), 2014

    2014-01-01

    Individualized Education Program (IEP) Facilitation is an optional process, not required by the Individuals with Disabilities Education Act (IDEA), that state educational agencies (SEA) or school districts may provide to parents and schools. The goal of a Facilitated IEP meeting is to develop an IEP that is supported by team members and benefits…

  11. Beyond Legal: Crafting High-Quality IEPs for Children With Complex Communication Needs

    Science.gov (United States)

    Rowland, Charity Mary; Quinn, Emily Dayle; Steiner, Sandra A. M.

    2015-01-01

    The Individualized Education Program (IEP) is a legal document developed for each student with a disability. The IEP outlines the student's learning needs and associated educational goals, as well as the program placement and services required to support the attainment of these goals in the least restrictive environment. Most IEPs include all…

  12. Toekomst voor de iep : Resistentie, vermeerdering en gebruikswaarde van het huidige sortiment

    NARCIS (Netherlands)

    Hiemstra, J.A.; Buitenveld [sic], J.; Kopinga, J.

    2012-01-01

    De iep was aan het begin van de 20e eeuw de belangrijkste laanboomsoort in Nederland. Door de problemen met iepziekte nam het belang van de iep in de loop van de 20e eeuw sterk af. De iep is echter een ideale laan-en straatboom die in grote delen van Nederland niet gemist kan worden. Inmiddels zijn

  13. Tablet Technology to Monitor Physical Education IEP Goals and Benchmarks

    Science.gov (United States)

    Lavay, Barry; Sakai, Joyce; Ortiz, Cris; Roth, Kristi

    2015-01-01

    The Individual with Disabilities Education Act (IDEA) mandates that all children who are eligible for special education services receive an individualized education program (IEP). Adapted physical education (APE) professionals who teach physical education to children with disabilities are challenged with how to best collect and monitor student…

  14. Legal IEPs: A Common Sense Approach with Barbara Bateman. [Videotape].

    Science.gov (United States)

    Council for Exceptional Children, Reston, VA. Div. for Learning Disabilities.

    In this 2-hour videotape workshop designed for teachers, administrators, parents, and others, Dr. Barbara Bateman answers many key questions that have been raised about Individualized Education Programs (IEPs) since the 1997 amendments to the Individuals with Disabilities Education Act (IDEA) and the 1999 regulations. The videotape reviews the…

  15. Transformational Leadership in Special Education: Leading the IEP Team

    Science.gov (United States)

    Lentz, Kirby

    2012-01-01

    Using the principles of transformational leadership, IEP teams become effective tools to ensure student success and achievements. There is a difference of teams that are simply chaired and those that are lead. Teams with transformational leaders promote the best efforts of all participants including parents and students to effectively deliver…

  16. Student IEP Participation and Academic Achievement across Time

    Science.gov (United States)

    Barnard-Brak, Lucy; Lechtenberger, DeAnn

    2010-01-01

    The Individuals with Disabilities Education Act mandates that students with disabilities be provided the necessary special education and related services that will allow them the benefit of a free and appropriate public education. Individualized Education Programs (IEPs) are the product of a team planning process that facilitates the coordination…

  17. Student and Parent IEP Collaboration: A Comparison across School Settings

    Science.gov (United States)

    Williams-Diehm, Kendra L.; Brandes, Joyce A.; Chesnut, Pik Wah; Haring, Kathryn A.

    2014-01-01

    The purpose of this study was to determine if differences existed across rural, urban, and suburban environments when special education teachers reported perceived levels of student and parent involvement and participation during IEP meetings. The investigators surveyed special education teachers (N = 159) across a Southwest state and applied log…

  18. The HAWK Highway: A Vertical Model for Student IEP Participation

    Science.gov (United States)

    Quann, Monica; Lyman, Jennifer; Crumlish, Jamie; Hines, Sally; Williams, Lynn; Pleet-Odle, Amy; Eisenman, Laura

    2015-01-01

    Special educators at an inclusive career-technical high school created a model to support annually increasing expectations for self-determination and levels of student participation in Individualized Education Program (IEP) planning and implementation. The grade-specific components of the model and supporting context are described. Students were…

  19. Belang en toekomst van de iep in Nederland

    NARCIS (Netherlands)

    Hiemstra, J.A.; Buiteveld, J.; Kopinga, J.; Kranenborg, K.G.; Ravesloot, M.B.M.; Sluis, van der B.J.; Vries, de S.M.G.

    2006-01-01

    In dit rapport het resultaat van een door PPO Bomen en Alterra uitgevoerde orienterende studie in opdracht van het PT naar de rol, het belang en de toekomstmogelijkheden van de iep in Nederland. Hierbij aandacht voor de in Nederland inheemse iepensoorten en hun eigenschappen, de gebruikswaarde van

  20. Iep of Olm - Karakterboom van de Lage Landen -

    NARCIS (Netherlands)

    Heybroek, H.; Goudzwaard, L.; Kaljee, H.

    2009-01-01

    Iep of Olm – karakterboom van de Lage Landen vertelt het verhaal van deze karakteristieke bomen in het licht van 7500 jaar Nederlandse cultuurgeschiedenis. Het boek geeft een historisch overzicht van iepen in stad en landschap, vertelt over oeroude gebruiken en schetst de geschiedenis van een eeuw

  1. Characterization of Agrobacterium species by capillary isoelectric focusing

    Czech Academy of Sciences Publication Activity Database

    Süle, S.; Horká, Marie; Matoušková, H.; Kubesová, Anna; Šalplachta, Jiří; Horký, J.

    2012-01-01

    Roč. 132, č. 1 (2012), s. 81-89 ISSN 0929-1873 R&D Projects: GA AV ČR IAAX00310701 Institutional research plan: CEZ:AV0Z40310501 Keywords : capillary isoelectric focusing * Agrobacterium spp. * identification Subject RIV: CB - Analytical Chemistry, Separation Impact factor: 1.610, year: 2012

  2. Simple power supply for power load controlled isoelectric focusing

    Czech Academy of Sciences Publication Activity Database

    Duša, Filip; Šlais, Karel

    2014-01-01

    Roč. 35, č. 8 (2014), s. 1114-1117 ISSN 0173-0835 R&D Projects: GA MV VG20102015023 Institutional support: RVO:68081715 Keywords : isoelectric focusing * power supply * voltage multiplier Subject RIV: CB - Analytical Chemistry, Separation Impact factor: 3.028, year: 2014 http://hdl.handle.net/11104/0231022

  3. New azo dyes as colored isoelectric point markers for isoelectric focusing in acidic pH region

    Czech Academy of Sciences Publication Activity Database

    Šťastná, Miroslava; Trávníček, Martin; Šlais, Karel

    2005-01-01

    Roč. 26, č. 1 (2005), s. 53-59 ISSN 0173-0835 R&D Projects: GA AV ČR(CZ) IBS4031201; GA ČR(CZ) GA203/02/1447; GA AV ČR IAA4031302 Keywords : ampholytic dyes * pI markers Subject RIV: CB - Analytical Chemistry, Separation Impact factor: 3.850, year: 2005

  4. The Effects of Using the Transition Planning Inventory and a Structured IEP Process as a Transition Planning Intervention on IEP Meeting Outcomes

    Science.gov (United States)

    Rehfeldt, Jason D.; Clark, Gary M.; Lee, Steven W.

    2012-01-01

    This study examined two areas. First, the authors looked at whether secondary individualized education program (IEP) teams who used the "Transition Planning Inventory" (TPI) in conjunction with a structured IEP meeting that based discussions and decisions on TPI data as a planning intervention generated significantly more…

  5. Perceptions of Self-Determination by Special Education and Rehabilitation Practitioners Based on Viewing a Self-Directed IEP versus an External-Directed IEP Meeting

    Science.gov (United States)

    Branding, Dave; Bates, Paul; Miner, Craig

    2009-01-01

    This study investigated perception of self-determination by special education and rehabilitation practitioners following their exposure to a videotaped simulation of a self-directed IEP meeting and an external-directed IEP meeting involving an adolescent with mild mental retardation. Groups of special education practitioners and rehabilitation…

  6. Human Brain Activity Patterns beyond the Isoelectric Line of Extreme Deep Coma

    Science.gov (United States)

    Kroeger, Daniel; Florea, Bogdan; Amzica, Florin

    2013-01-01

    The electroencephalogram (EEG) reflects brain electrical activity. A flat (isoelectric) EEG, which is usually recorded during very deep coma, is considered to be a turning point between a living brain and a deceased brain. Therefore the isoelectric EEG constitutes, together with evidence of irreversible structural brain damage, one of the criteria for the assessment of brain death. In this study we use EEG recordings for humans on the one hand, and on the other hand double simultaneous intracellular recordings in the cortex and hippocampus, combined with EEG, in cats. They serve to demonstrate that a novel brain phenomenon is observable in both humans and animals during coma that is deeper than the one reflected by the isoelectric EEG, and that this state is characterized by brain activity generated within the hippocampal formation. This new state was induced either by medication applied to postanoxic coma (in human) or by application of high doses of anesthesia (isoflurane in animals) leading to an EEG activity of quasi-rhythmic sharp waves which henceforth we propose to call ν-complexes (Nu-complexes). Using simultaneous intracellular recordings in vivo in the cortex and hippocampus (especially in the CA3 region) we demonstrate that ν-complexes arise in the hippocampus and are subsequently transmitted to the cortex. The genesis of a hippocampal ν-complex depends upon another hippocampal activity, known as ripple activity, which is not overtly detectable at the cortical level. Based on our observations, we propose a scenario of how self-oscillations in hippocampal neurons can lead to a whole brain phenomenon during coma. PMID:24058669

  7. Individualized Education Program (IEP Mata Pelajaran Kimia untuk Siswa Slow Learner

    Directory of Open Access Journals (Sweden)

    Rovik Rovik

    2017-06-01

    [Siswa slow learner menempati populasi tertinggi untuk siswa berkebutuhan khusus. Sebagai salah satu jenis learning disability, slow learner masih dapat belajar dengan teman sebayanya asalkan guru mempersiapkan program pembelajaran khusus yang telah dimodifikasi dari pembelajaran reguler. Program ini disebut Individualized Education Program (IEP. Penelitian ini mencoba mengembangkan IEP mata pelajaran kimia untuk slow learner, mengidentifikasi komponen yang dibutuhkan dalam menyusun IEP untuk slow learner, dan menganalisis judgement reviewers (guru kimia dan guru pendamping khusus dan peer reviewers terhadap IEP yang dikembangkan. Hasil penelitian menunjukkan slow learner membutuhkan IEP sebagai dokumen utama panduan guru dalam pembelajaran kimia di kelas. Komponen pengembangan IEP meliputi identitas peserta didik, tim pengembangan dan pelaksana, asesmen yang pernah dilakukan, hambatan dan kekuatan, kebutuhan dan perlakuan, faktor pendukung dan penghambat, rencana perlakuan, dan modifikasi terhadap perangkat pembelajaran kimia reguler

  8. Preparative isoelectric focusing in a cellulose-based separation medium

    Czech Academy of Sciences Publication Activity Database

    Šalplachta, Jiří; Horká, Marie; Šlais, Karel

    2017-01-01

    Roč. 40, č. 11 (2017), s. 2498-2505 ISSN 1615-9306 R&D Projects: GA MZd(CZ) NV16-29916A; GA ČR(CZ) GA16-03749S; GA MV(CZ) VI20172020069 Institutional support: RVO:68081715 Keywords : isoelectric focusing * preparative * proteins * separation Subject RIV: CB - Analytical Chemistry, Separation OBOR OECD: Analytical chemistry Impact factor: 2.557, year: 2016

  9. Compilation of PZC and IEP of sparingly soluble metal oxides and hydroxides from literature.

    Science.gov (United States)

    Kosmulski, Marek

    2009-11-30

    The values of PZC and IEP of metal oxides reported in the literature are affected by the choice of the specimens to be studied. The specimens, which have PZC and IEP similar to the "recommended" value, are preferred by the scientists. The biased choice leads to accumulation of results for a few specimens, and the other specimens are seldom studied or they are subjected to washing procedures aimed at shift of the original IEP toward the "recommended" value. Taking the average or median of all published PZC and IEP for certain oxide as the "recommended" value leads to substantiation of previously published results due to overrepresentation of certain specimens in the sample.

  10. Local anti-fertility effect of inhibin-enriched preparation (IEP) in female hamsters.

    Science.gov (United States)

    Bapat, B V; Nandedkar, T D; Sheth, A R

    1984-04-01

    An inhibin-enriched preparation (IEP) involved in the regulation of follicle stimulating hormone (FSH) is known to play an important role in the normal ovarian cycle. In utero administration of 10 micrograms of IEP on day 3 of pregnancy completely prevented implantation in hamsters. No toxic effect of IEP was observed on the blastocysts as indicated by the dye exclusion test performed with Trypan blue. Thus, the results of the present study indicate an extra-pituitary site of action for the anti-implantation effect of IEP.

  11. A comparative analysis of phloem exudate proteins from Cucumis melo, Cucumis sativus and Cucurbita maxima by polyacrylamide gel electrophoresis and isoelectric focusing.

    Science.gov (United States)

    Sabnis, D D; Hart, J W

    1976-01-01

    Proteins in sieve tube exudate from Cucumis melo L., Cucumis sativus L. and Cucurbita maxima Duch. were analysed by gel electrophoresis and isoelectric focusing. Estimated molecular weights and isoelectric points for the major and minor proteins from each plant species are presented. Electrophoresis revealed striking differences between the protein complements of exudatc from the two genera investigated. Similarly, although a few exudate proteins from the two species of Cucumis possessed identical molecular weights, several major proteins were peculiar to each species. Isoelectric focusing of proteins in exudate samples from the three plants confirmed the marked differences in their protein complements. Furthermore, focusing also revealed differences between cultivars of Cucumis sativus. Both Cucumis sativus and Cucurbita maxima possessed relatively large amounts of basic proteins; these were absent in exudate from Cucumis melo. The implications of these results are discussed in relation to present concepts regarding the interrelationships and possible functional roles of P-proteins.

  12. BC Hydro's integrated resource planning : the 2004 IEP and beyond

    International Nuclear Information System (INIS)

    Soulsby, R.

    2004-01-01

    An outline of BC Hydro Integrated Electricity Plan (IEP) was presented in this paper, along with details of its environmental, social and business performance and statistics of its net income and revenue for 2003-2004. The IEP was created to match long term load to supply in the most cost-effective way and is also the basis for resource acquisition plans. In addition, the IEP models performance of portfolios of resources on a system-wide basis, considers uncertainties through sensitivity analysis and measures and evaluates multiple attributes from various portfolios. A flow chart of processes informed by the IEP was presented. Guiding principles behind the creation of the plan include low electricity rates and public ownership; secure, reliable supply; private sector development; environmental responsibility, with no nuclear power sources; ensuring energy self-sufficiency; and maintaining a balanced portfolio of resources. A pie chart of BC Hydro's current portfolio mix was presented as well as a supply and demand outlook and details of plans and new capacity resources required before 2013. Various resource options were presented. Key outcomes of First Nations and stakeholder engagement include the acceptance of BC Hydro as a sustainable energy company; a desire for higher priority on reliability and low cost; an agreement over the Power Smart 10 year plan; a general agreement that the current, balanced approach to resource acquisition was appropriate; and a desire for more rate options. There was an assurance that BC Hydro would continue to engage First Nations and stakeholders in integrated electricity planning. Other outcomes included requests for more information about outage and outage planning and process improvements; support to maintain low rates; a preference for maximizing use of existing facilities; support for wood waste cogeneration. A broad range of options were reviewed, but no superior portfolio was identified within the parameters of this paper

  13. Differential potentiometric titration: development of a methodology for determining the point of zero charge of metal (hydr)oxides by one titration curve.

    Science.gov (United States)

    Bourikas, Kyriakos; Kordulis, Christos; Lycourghiotis, Alexis

    2005-06-01

    A new methodology is presented, called differential potentiometric titration (DPT), which allows the determination of the point of zero charge (pzc) of metal (hydr)oxides using only one potentiometric curve. By performing extensive simulations of potentiometric titrations for various model (hydr)oxides, we found that an inflection point in a H+(cons,surf) versus pH potentiometric curve (H+(cons,surf): hydrogen ions consumed on the surface of the (hydr)oxide) and a peak in the corresponding differential curve, dH+(cons,surf)/dpH versus pH, appear at a pH equal to the pzc assumed for a model (hydr)oxide. This distinguishable peak appears at the same position irrespective of the surface ionization and the interfacial model adopted as well as the assumed ionic strength. It was found that the aforementioned peak also appears in the high-resolution differential potentiometric curves experimentally determined for four oxides (SiO2, TiO2, gamma-Al2O3, and MgO) that are widely used in various environmental and other technological applications. The application of DPT to the above-mentioned oxides provided practically the same pzc values as the corresponding ones achieved by using four different techniques as well as the corresponding isoelectric point (iep) values determined by microelectrophoresis. Differences between the pzc and iep values determined using various techniques in the case of MgO were attributed to the increasing dissolution of this oxide as pH decreases and the adsorption of cations (Mg2+, Na+) on the MgO/electrolytic solution interface.

  14. Correlation Between Physicochemical Characteristics and Toxicological Properties of Nanomaterials

    Science.gov (United States)

    2012-01-25

    the isoelectric point ( IEP ) which is important for toxicological investigation as discussed earlier. The nanoparticles all synthesized by flame...that dopant addition can change surface charge and hydrodynamic diameter and also shift the IEP to higher or lower pH than pristine TiO2, depending on...electric point ( IEP ) compared to the pristine TiO2. Vanadium and platinum doping shifted the IEP to lower pH values, whereas copper doping shifted it to

  15. Understanding, Developing, and Writing Effective IEPs: A Step-by-Step Guide for Educators

    Science.gov (United States)

    Pierangelo, Roger; Giuliani, George A.

    2007-01-01

    Creating and evaluating Individualized Education Programs (IEPs) for students with disabilities is a major responsibility for teachers and school leaders, yet the process involves legal components not always understood by educators. In "Understanding, Developing, and Writing Effective IEPs," legal and special education experts Roger…

  16. Culturally and Linguistically Diverse Parents' Perceptions of the IEP Process

    Science.gov (United States)

    Wolfe, Katie; Durán, Lillian K.

    2013-01-01

    Many parents of students with disabilities face barriers to meaningful participation in Individualized Education Plan (IEP) meetings; parents who are culturally and linguistically diverse (CLD) encounter additional challenges. Given the changing demographics of the United States and the central role of the IEP in special education, it is important…

  17. The IEP Meeting: Perceptions of Parents of Students Who Receive Special Education Services

    Science.gov (United States)

    Fish, Wade W.

    2008-01-01

    The author investigated parental perceptions of the individualized education program (IEP) meeting among 51 parents of students who were receiving special education services from 1 family support service agency. Survey questions pertained to the following areas: (a) IEP meeting experiences, (b) knowledge level of special education law, (c)…

  18. Weer volop toekomst voor de iep Nieuwe iepen: zeer hoge mate van resistentie en algemeen bruikbaar

    NARCIS (Netherlands)

    Hiemstra, J.A.

    2013-01-01

    De iep is eeuwenlang een van de beeldbepalende bomen geweest in het Nederlandse landschap en de Nederlandse steden. Door de opkomst van de iepziekte zijn in de loop van de vorige eeuw veel van deze Hollandse iepen verdwenen. Door de voortdurende problemen met de iepziekte kreeg de iep bij veel

  19. Facilitated IEP Meetings. PACER Center ACTion Information Sheets: PHP-c90

    Science.gov (United States)

    PACER Center, 2014

    2014-01-01

    To help special education planning teams reach agreements, the Minnesota Department of Education, Compliance and Assistance, Alternative Dispute Resolution Services provide the option of facilitated IEP (Individualized Education Program) meetings. This option is available for IEP, IIIP (Individual Interagency Intervention Plan), and IFSP…

  20. Teachers' Perceptions of the Efficacy of Standards-Based IEP Goals

    Science.gov (United States)

    Smith, Traci Nicole

    2013-01-01

    Although standards-based IEP goals have been mandated in many states for almost a decade, their effectiveness is unknown. Standards-based IEP goals were first created to meet the requirements of No Child Left Behind and Individuals with Disabilities Education Improvement Act, which increased accountability for all students as well as those with…

  1. The Relationship of IEP Quality to Curricular Access and Academic Achievement for Students with Disabilities

    Science.gov (United States)

    La Salle, Tamika P.; Roach, Andrew T.; McGrath, Dawn

    2013-01-01

    The purpose of this study was to investigate the quality of Individualized Education Programs (IEPs) and its influence on academic achievement, inclusion in general education classrooms, and curricular access for students with disabilities. 130 teachers from the state of Indiana were asked to submit the most recent IEP of one of their students in…

  2. Self-Advocacy Skills as a Predictor of Student IEP Participation among Adolescents with Autism

    Science.gov (United States)

    Barnard-Brak, Lucy; Fearon, Danielle D.

    2012-01-01

    The importance of student IEP participation has been indicated by both legislative mandates such as IDEA and research literature. The purpose of the current study was to examine those variables that predict student IEP participation among adolescents with autism spectrum disorders as compared to adolescents with disabilities other than autism…

  3. Which Procedural Parts of the IEP Process Are the Most Judicially Vulnerable?

    Science.gov (United States)

    Zirkel, Perry A.; Hetrick, Allyse

    2017-01-01

    To provide a missing piece to the legal foundation of professional development and practice for the individualized education program (IEP) process, the authors report the results of a comprehensive systematic analysis of court decisions specific to IEP-related procedural violations after the 2004 amendments of the Individuals With Disabilities…

  4. Let's Get Parents Ready for Their Initial IEP Meeting

    Science.gov (United States)

    Hammond, Helen; Ingalls, Lawrence

    2017-01-01

    Parental participation in the initial Individual Education Program (IEP) meeting is a critical component of the process. Even though parents have rights to be equally involved in making decisions at the IEP meetings, frequently parents aren't prepared to be equal members on the team with school personnel. This study focused on a preparation…

  5. Hearing Parents of Children with Hearing Loss: Perceptions of the IEP Process

    Science.gov (United States)

    Stegman, Robin Fern

    2016-01-01

    Under federal guidelines, parents of school-aged children with hearing loss are required to attend an individualized education program (IEP) meeting on behalf of their child. However, it remains unclear how prepared hearing parents are to oversee development of IEPs that guarantee their children the best educational outcomes, as well as how much…

  6. The Impact of Professional Development on the Quality of the Transition Components of IEPs

    Science.gov (United States)

    Flannery, K. Brigid; Lombardi, Allison; Kato, Mimi McGrath

    2015-01-01

    Under the Individuals With Disabilities Education Act (IDEA), transition needs and services are to be discussed as part of the Individual Education Program (IEP) planning process, and decisions based on students' future goals are to be documented in the IEP. These transition requirements were included in IDEA in order to plan with the student,…

  7. The IEP from A to Z: How to Create Meaningful and Measurable Goals and Objectives

    Science.gov (United States)

    Twachtman-Cullen, Diane; Twachtman-Bassett, Jennifer

    2011-01-01

    With the skyrocketing diagnoses of ADHD, autism spectrum disorders, and related conditions in U.S. schools, there is a growing need for information on creating effective IEPs for exceptional students. The "IEP From A to Z" is a step-by-step guide showing teachers and parents how to get the right education plan in place for students with ADHD,…

  8. An Investigation into the Involvement of California Central Valley High School Students with Disabilities in the IEP Process

    Science.gov (United States)

    Anderson, Cheryle Ann

    2012-01-01

    The purpose of this study was to investigate the involvement of California Central Valley high school students with disabilities in the Individual Education Plan (IEP) process. Specifically, this study investigated the involvement of students with disabilities in the development of the IEP and IEP meetings. In addition, this study explored the…

  9. Perceptions of Parents of Students with Autism towards the IEP Meeting: A Case Study of One Family Support Group Chapter

    Science.gov (United States)

    Fish, Wade W.

    2006-01-01

    This case study investigated parental perceptions of students with autism towards the IEP meeting from one family support group chapter in the north Texas area. Participants were asked to share their experiences of previous IEP meetings and to provide input regarding not only measures that school districts may take towards improving IEP meetings,…

  10. Immunoperoxidase staining and radioimmunobinding of human tumor markers separated by direct tissue agarose isoelectric focusing

    International Nuclear Information System (INIS)

    Saravis, C.A.; Cunningham, C.G.; Marasco, P.V.; Cook, R.B.; Zamcheck, N.; FMC Corp., Rockland, ME

    1980-01-01

    The new technique of agarose isoelectric focusing is used to identify, quantitate, and characterize specific tumor markers. After fixation of the isoelectric focusing patterns these are reacted with specific anti-tumor marker antisera, then with second antibody either peroxidase conjugated or radiolabellad (radioiodine). (RB) [de

  11. Perceptions of self-determination by special education and rehabilitation practitioners based on viewing a self-directed IEP versus an external-directed IEP meeting.

    Science.gov (United States)

    Branding, Dave; Bates, Paul; Miner, Craig

    2009-01-01

    This study investigated perception of self-determination by special education and rehabilitation practitioners following their exposure to a videotaped simulation of a self-directed IEP meeting and an external-directed IEP meeting involving an adolescent with mild mental retardation. Groups of special education practitioners and rehabilitation practitioners did not differ from each other in their perceptions of self-determination before or after viewing either the self-directed or external-directed IEP meeting simulation. However, both groups of respondents had higher perceptions of the self-determination capability of the confederate student when they viewed her in a self-directed meeting. In addition, respondents consistently rated the self-directed meeting simulation as being of higher overall quality than the external-directed meeting. Results are discussed in relation to practitioner recommendations and future research in regard to the development and enabling of self-determination skills involving persons with disabilities.

  12. The isoelectric region of proteins: a systematic analysis.

    Directory of Open Access Journals (Sweden)

    Michael Widmann

    Full Text Available BACKGROUND: Binding of proteins in ion exchange chromatography is dominated by electrostatic interactions and can be tuned by adjusting pH and ionic strength of the solvent. Therefore, the isoelectric region (IER, the pH region of almost zero charge near the pI, has been used to predict the binding properties of proteins. PRINCIPAL FINDINGS: Usually the IER is small and binding and elution is carried out at pH values near to the pI. However, some proteins with an extended IER have been shown to bind and elute far away from its pI. To analyze factors that mediate the size of the IER and to identify proteins with an extended IER, two protein families consisting of more than 7000 proteins were systematically investigated. Most proteins were found to have a small IER and thus are expected to bind or elute near to their pI, while only a small fraction of less than 2% had a large IER. CONCLUSIONS: Only four factors, the number of histidines, the pI, the number of titratable amino acids and the ratio of acidic to basic residues, are sufficient to reliably classify proteins by their IER based on their sequence only, and thus to predict their binding and elution behaviour in ion exchange chromatography.

  13. Cloning and characterization of Bacillus subtilis iep, which has positive and negative effects on production of extracellular proteases.

    Science.gov (United States)

    Tanaka, T; Kawata, M

    1988-08-01

    We have isolated a DNA fragment from Bacillus subtilis 168 which, when present in a high-copy plasmid, inhibited production of extracellular alkaline and neutral proteases. The gene responsible for this activity was referred to as iep. The open reading frame of iep was found to be incomplete in the cloned DNA fragment. When the intact iep gene was reconstructed after the missing part of the iep gene had been cloned, it showed an enhancing effect on the production of the extracellular proteases. The open reading frame encodes a polypeptide of 229 amino acids with a molecular weight of ca. 25,866. Deletion of two amino acids from the N-terminal half of the putative iep protein resulted in dual effects, i.e., a decrease in the inhibitory activity shown by the incomplete iep gene and a slight increase in the enhancing activity shown by the complete iep gene. These results show that the iep gene product is a bifunctional protein, containing inhibitory and enhancing activities for the exoprotease production in the N-terminal and C-terminal regions, respectively. It was found by genetic and functional analyses that iep lies very close to sacU.

  14. IEP goals for school-age children with speech sound disorders.

    Science.gov (United States)

    Farquharson, Kelly; Tambyraja, Sherine R; Justice, Laura M; Redle, Erin E

    2014-01-01

    The purpose of the current study was to describe the current state of practice for writing Individualized Education Program (IEP) goals for children with speech sound disorders (SSDs). IEP goals for 146 children receiving services for SSDs within public school systems across two states were coded for their dominant theoretical framework and overall quality. A dichotomous scheme was used for theoretical framework coding: cognitive-linguistic or sensory-motor. Goal quality was determined by examining 7 specific indicators outlined by an empirically tested rating tool. In total, 147 long-term and 490 short-term goals were coded. The results revealed no dominant theoretical framework for long-term goals, whereas short-term goals largely reflected a sensory-motor framework. In terms of quality, the majority of speech production goals were functional and generalizable in nature, but were not able to be easily targeted during common daily tasks or by other members of the IEP team. Short-term goals were consistently rated higher in quality domains when compared to long-term goals. The current state of practice for writing IEP goals for children with SSDs indicates that theoretical framework may be eclectic in nature and likely written to support the individual needs of children with speech sound disorders. Further investigation is warranted to determine the relations between goal quality and child outcomes. (1) Identify two predominant theoretical frameworks and discuss how they apply to IEP goal writing. (2) Discuss quality indicators as they relate to IEP goals for children with speech sound disorders. (3) Discuss the relationship between long-term goals level of quality and related theoretical frameworks. (4) Identify the areas in which business-as-usual IEP goals exhibit strong quality.

  15. Attitudes of Special Education Teachers and School Psychologists toward Individualized Education Plan IEPs Developed Using Traditional Assessments versus IEPs Developed Using a Multiple Intelligence Assessment

    Science.gov (United States)

    Alhajri, Meshari A SH A.

    2011-01-01

    The purpose of this research was to determine the usefulness of Multiple Intelligence for educational planning for students in special education. More specifically, this study applied the Multiple Intelligences Developmental Assessment Scales (MIDAS) to a sample of students receiving special education services who had IEPs developed using…

  16. Two-dimensional capillary electrophoresis: capillary isoelectric focusing and capillary zone electrophoresis with laser-induced fluorescence detection

    Science.gov (United States)

    Dickerson, Jane A.; Ramsay, Lauren M.; Dada, Oluwatosin O.; Cermak, Nathan

    2011-01-01

    Capillary isoelectric focusing and capillary zone electrophoresis are coupled with laser-induced fluorescence detection to create an ultrasensitive two-dimensional separation method for proteins. In this method, two capillaries are joined through a buffer filled interface. Separate power supplies control the potential at the injection end of the first capillary and at the interface; the detector is held at ground potential. Proteins are labeled with the fluorogenic reagent Chromeo P503, which preserves the isoelectric point of the labeled protein. The labeled proteins were mixed with ampholytes and injected into the first dimension capillary. A focusing step was performed with the injection end of the capillary at high pH and the interface at low pH. To mobilize components, the interface was filled with a high pH buffer, which was compatible with the second dimension separation. A fraction was transferred to the second dimension capillary for separation. The process of fraction transfer and second dimension separation was repeated two dozen times. The separation produced a spot capacity of 125. PMID:20603830

  17. Species identification of smoked and gravad fish products by sodium dodecylsulphate polyacrylamide gel electrophoresis, urea isoelectric focusing and native isoelectric focusing : a collaborative study

    DEFF Research Database (Denmark)

    Mackie, I.; Craig, A.; Etienne, M.

    2000-01-01

    A collaborative study on the use of sodium dodecylsulphate polyacrylamide gel electrophoresis (SDS-PAGE), urea-isoelectric focusing (urea-IEF) and native isoelectric focusing for the identification of species of smoked salmonids, gravad salmonids and smoked eels was carried out by eight laborator......A collaborative study on the use of sodium dodecylsulphate polyacrylamide gel electrophoresis (SDS-PAGE), urea-isoelectric focusing (urea-IEF) and native isoelectric focusing for the identification of species of smoked salmonids, gravad salmonids and smoked eels was carried out by eight...... laboratories. With SDS-PAGE, minor changes took place in the profiles of the processed salmonid species making it impossible or Very difficult to identify closely related species. With urea-IEF, there were fewer changes in the profiles due to processing and the system generally had greater species......-discriminating power for the processed salmonids than SDS-PAGE. The profiles of the eel species as obtained on SDS-PAGE or urea-IEF were not affected by smoking. Urea-IEF had greater species- discriminating power than SDS-PAGE for the eel species. Native IEF was useful in providing supplementary identification...

  18. Do the symptoms of language disorder align with treatment goals? An exploratory study of primary-grade students' IEPs.

    Science.gov (United States)

    Schmitt, Mary Beth; Justice, Laura M; Logan, Jessica A R; Schatschneider, Christopher; Bartlett, Christopher W

    2014-01-01

    Individual Education Plans (IEPs) are legal documents that guide the treatment of students with language disorder (LD). This exploratory study investigated the extent to which students' symptoms of LD align with goals on their IEPs. A total of 99 kindergarten and first-grade students receiving treatment for LD in the public schools participated. IEPs were collected and coded for each student and norm-referenced measures were used to assess students' grammar, vocabulary, listening comprehension, and literacy skills in the fall of the academic year. Results showed there to be alignment between students' symptoms and IEP goals only in the area of vocabulary, such that students who had an IEP goal for vocabulary had lower scores on a vocabulary assessment than those without a goal. In general, there is limited alignment between observed symptoms of LD and treated symptoms as identified on students' IEPs. The limited alignment found in this study suggests more investigation is needed to understand the extent to which IEP goals, as potential indicators of treatment foci, should map on to students' symptoms. Readers will be able to: (1) explain the theoretical and practical relevance of treatment goals aligning to symptoms for children with language impairment; (2) identify three analytic methods used to investigate alignment between treatment goals and symptoms; and (3) describe the extent to which IEP goals align to children's symptoms in a sample of children receiving services in the public schools. Copyright © 2014 Elsevier Inc. All rights reserved.

  19. The Development and Evaluation of a Professional Development Model to Build Meaningful and Effective IEPs for Transition-Aged Students

    Science.gov (United States)

    Doren, Bonnie; Flannery, K. Brigid; Lombardi, Allison

    2012-01-01

    The purpose of the study was to examine the potential efficacy of a professional development training model targeting IEP case managers of transition-age students. A training model was developed and a pilot study conducted to understand the promise of the model to improve the development of critical components within the IEP document that support…

  20. Structured Intervention as a Tool to Shift Views of Parent-Professional Partnerships: Impact on Attitudes toward the IEP

    Science.gov (United States)

    Mereoiu, Mariana; Abercrombie, Sarah; Murray, Mary M.

    2016-01-01

    The Individualized Education Program (IEP) is the roadmap that helps educators and families drive the education of students with disabilities, improve outcomes, and fulfill each child's potential. However, the IEP can be challenging due to the large number and diversity of stakeholders, dynamics and culture of collaboration, and the complex…

  1. Writing IEPs for the Audience of Teachers, Parents, and Students: The Case for the Communicative Individual Education Program

    Science.gov (United States)

    Lucido, Richard J.

    2013-01-01

    Our current paradigm of Individualized Education Program (IEP) construction, the creation of a legal document or contract between parents and schools, needs rethinking. This paradigm's unintended consequences have resulted in vast inefficiencies in special education and have detracted from the core purpose of the IEP document, the communication of…

  2. Accessing the Common Core Standards for Students with Learning Disabilities: Strategies for Writing Standards-Based IEP Goals

    Science.gov (United States)

    Caruana, Vicki

    2015-01-01

    Since the reauthorization of the Individuals With Disabilities Education Act (IDEA) in 2004, standards-based individualized education plans (IEPs) have been an expectation for serving students with disabilities in the K-12 public school setting. Nearly a decade after the mandates calling for standards-based IEPs, special educators still struggle…

  3. CORPORATE STRATEGY: RELATIONSHIP BETWEEN THE IDP, IEP AND PLANNING STRATEGIC IN IFB

    Directory of Open Access Journals (Sweden)

    Pedro Henrique Rodrigues de Camargo Dias

    2017-08-01

    Full Text Available This study aims to identify and characterize the relationship of the Institutional Development Plan - IDP -, Institutional Educational Project - IEP, - and the strategic planning, specifically in the context of organizational effectiveness.  IDP, IEP and Strategic Planning at the Federal Institution of Brasilia (Instituto Federal de Brasilia IFB interrelate and interact due to its strategic nature and focus on organizational results. The qualitative study of applied nature and exploratory personality, is instrumentalized by documentary techniques  and semi-structured interviews and content analysis. The results showed that the efficient integration of IDP, IEP and Strategic Planning, and also communicate the mission, objectives and institutional goals, corroborate the construction of a reference institution with the quality of education, stating its social function, guiding the action of servers and managers.

  4. Teacher and child predictors of achieving IEP goals of children with autism.

    Science.gov (United States)

    Ruble, Lisa; McGrew, John H

    2013-12-01

    It is encouraging that children with autism show a strong response to early intervention, yet more research is needed for understanding the variability in responsiveness to specialized programs. Treatment predictor variables from 47 teachers and children who were randomized to receive the COMPASS intervention (Ruble et al. in The collaborative model for promoting competence and success for students with ASD. Springer, New York, 2012a) were analyzed. Predictors evaluated against child IEP goal attainment included child, teacher, intervention practice, and implementation practice variables based on an implementation science framework (Dunst and Trivette in J Soc Sci 8:143-148, 2012). Findings revealed one child (engagement), one teacher (exhaustion), two intervention quality (IEP quality for targeted and not targeted elements), and no implementation quality variables accounted for variance in child outcomes when analyzed separately. When the four significant variables were compared against each other in a single regression analysis, IEP quality accounted for one quarter of the variance in child outcomes.

  5. Islam - Science Integration Approach in Developing Chemistry Individualized Education Program (IEP for Students with Disabilities

    Directory of Open Access Journals (Sweden)

    Jamil Suprihatiningrum

    2017-11-01

    Full Text Available The paper is based on a research which tries to explore, explain and describe Islam - science integration approach to develop an Individualized Education Program (IEP for students with disabilities in chemistry lesson. As a qualitative case study, this paper is aimed at investigating how Islam - science integration approach can be underpinned for developing the IEP for Chemistry. Participants were recruited purposively and data were collected by interviews; documents’ analysis; and experts’ assessment (i.e. material experts, inclusive education experts, media experts, chemistry teachers and support teachers, then analyzed using content-analysis. The result shows Islam - science integration approach can be a foundation to develop the chemistry IEP by seeking support for the verses of the Qur'an and corresponding hadiths. Even although almost all the subject matter in chemistry can be integrated with Islamic values, this study only developed two contents, namely Periodic System of Elements and Reaction Rate.

  6. Immunoevasive protein (IEP)-containing surface layer covering polydnavirus particles is essential for viral infection.

    Science.gov (United States)

    Furihata, Shunsuke; Tanaka, Kohjiro; Ryuda, Masasuke; Ochiai, Masanori; Matsumoto, Hitoshi; Csikos, Gyorge; Hayakawa, Yoichi

    2014-01-01

    Polydnaviruses (PDVs) are unique symbiotic viruses associated with parasitoid wasps: PDV particles are injected into lepidopteran hosts along with the wasp eggs and express genes that interfere with aspects of host physiology such as immune defenses and development. Recent comparative genomic studies of PDVs have significantly improved our understanding of their origin as well as the genome organization. However, the structural features of functional PDV particles remain ambiguous. To clear up the structure of Cotesia kariyai PDV (CkPDV) particles, we focused on immunoevasive protein (IEP), which is a mediator of immunoevasion by the wasp from the encapsulation reaction of the host insect's hemocytes, because it has been demonstrated to be present on the surface of the virus particle. We discovered that IEP tends to polymerize and constitutes a previously unidentified thin surface layer covering CkPDV particles. This outermost surface layer looked fragile and was easily removed from CkPVD particles by mechanical stressors such as shaking, which prevented CkPDV from expressing the encoded genes in the host target tissues such as fat body or hemocytes. Furthermore, we detected IEP homologue gene expression in the wasp's venom reservoirs, implying IEP has another unknown biological function in the wasp or parasitized hosts. Taken together, the present results demonstrated that female C. kariyai wasps produce the fragile thin layer partly composed of IEP to cover the outer surfaces of CkPDV particles; otherwise, they cannot function as infectious agents in the wasp's host. The fact that IEP family proteins are expressed in both venom reservoirs and oviducts suggests an intimate relationship between both tissues in the development of the parasitism strategy of the wasp. Copyright © 2013 Elsevier Inc. All rights reserved.

  7. Using laser technological unit ALTI "Karavella" for precision components of IEP production

    Science.gov (United States)

    Labin, N. A.; Chursin, A. D.; Paramonov, V. S.; Klimenko, V. I.; Paramonova, G. M.; Kolokolov, I. S.; Vinogradov, K. Y.; Betina, L. L.; Bulychev, N. A.; Dyakov, Yu. A.; Zakharyan, R. A.; Kazaryan, M. A.; Koshelev, K. K.; Kosheleva, O. K.; Grigoryants, A. G.; Shiganov, I. N.; Krasovskii, V. I.; Sachkov, V. I.; Plyaka, P. S.; Feofanov, I. N.; Chen, C.

    2015-12-01

    The paper revealed the using of industrial production equipment ALTI "Karavella-1", "Karavella-1M", "Karavella-2" and "Karavella-2M" precision components of IEP production [1-4]. The basis for the ALTI using in the IEP have become the positive results of research and development of technologies of foil (0.01-0.2 mm) and thin sheets (0.3-1 mm) materials micromachining by pulsed radiation CVL [5, 6]. To assess the micromachining quality and precision the measuring optical microscope (UHL VMM200), projection microscope (Mitutoyo PV5100) and Carl Zeiss microscope were used.

  8. [Randomized clinical trial of IEP and EP regimens in the treatment of patients with small cell lung cancer].

    Science.gov (United States)

    Zhou, Hui; Wang, Anlan; Huang, Zhihua; Zhou, Wenwei

    2004-06-20

    To observe and compare the efficacy and safety of IEP and EP regimens for small cell lung cancer (SCLC). Sixty-four patients with SCLC pathologically proved were randomly divided into IEP group ( n =32) and EP group ( n =32). All the 64 patients were evaluable for response and toxicity. In IEP group, the total responsive rate, responsive rates of limited-stage patients and extensive-stage patients were 84.4%(27/32), 100.0%(15/15) and 70.6%(12/17) respectively; while in EP group, those were 75.0%(24/32), 85.7%(12/14) and 66.7% (12/18) respectively. The median duration of remission was 6 months and 1-year survival rate was 62.5% in IEP group, and 5 months and 56.2% in EP group. There was no significant difference in response rate, median duration of remission and 1-year survival between the two groups ( P > 0.05). The main toxicity was myelosuppression. Incidences of leukopenia at grade III-IV, nausea, vomiting and alopecia were significantly higher in the IEP arm than those in the EP arm ( P IEP and EP. IEP regimen shows a similar response rate compared with EP regimen. They might be considered as relevant regimens in initial patients with small cell lung cancer.

  9. Toward Authentic IEPs and Transition Plans: Student, Parent, and Teacher Perspectives

    Science.gov (United States)

    Cavendish, Wendy; Connor, David

    2018-01-01

    This mixed-methods study examined perspectives on factors that influence meaningful student and parent involvement in Individualized Education Program (IEP) transition planning. Survey data and open-ended qualitative interviews with urban high school students with a learning disability (LD; n = 16), their parents (n = 9), and their teachers (n =…

  10. "Doug C. v. Hawaii Department of Education": Parental Participation in IEP Development

    Science.gov (United States)

    Yell, Mitchell L.; Katsiyannis, Antonis; Losinski, Mickey

    2015-01-01

    Parental participation is a crucial component of the Individuals with Disabilities Education Act. When developing students' Individualized Education Programs (IEPs), school-based teams must place a high priority on involving students' parents in a collaborative effort to develop their children's educational programs and determine their placements.…

  11. The Essential and Interrelated Components of Evidenced-Based IEPs: A User's Guide

    Science.gov (United States)

    Hauser, Marc D.

    2017-01-01

    Individualized education program (IEP) goals and objectives often provide illogical or insufficient methodological detail (e.g., Capizzi, 2008). Insufficient methods can lead to ambiguity among the staff members responsible for data collection or to data that fail to take into account factors that can affect performance or behavioral expression…

  12. State of the Art Student Support Services in an IEP Learning Center

    Science.gov (United States)

    Hanson, Jessica; Maxwell, Jeffrey; Mulder, Monika

    2015-01-01

    Intensive English language programs (IEPs) at American universities have the task of recruiting, retaining, and preparing international students for mainstream classes. In order to achieve these tasks, many programs have explored using supplemental instruction (SI) in the form of learning centers (LCs) to support their students. In this study, we…

  13. Collaboration during IEP and IFSP Meetings in a Refugee Resettlement Community: Lessons from Cultural Liaisons

    Science.gov (United States)

    Hurley, Jennifer J.; Clark, David W.; Fonseca-Foster, Katherine A.; Pyne, Sabina K.; Warren, Rachel A.

    2017-01-01

    Teachers working with refugee families who are culturally and linguistically diverse (CLD) and receiving special education services often rely on cultural liaisons to provide interpreter and translator services during Individualized Educational Program (IEP) and Individualized Family Service Plan (IFSP) meetings. The purpose of this qualitative…

  14. Background of Individual Education Plans (IEPs) Policy in Some Countries: A Review

    Science.gov (United States)

    Alkahtani, Mohammed Ali; Kheirallah, Sahar Abdelfattah

    2016-01-01

    This paper seeks to provide a cogent outline of the current policies that six separate countries have on Individual Education Plans (IEPs), identifying the key features in each system. The chosen countries are Australia (Queen Island), Canada (British Columbia), New Zealand, the United Kingdom, the United States of America, and Saudi Arabia. The…

  15. Improvement of radiological penumbra using intermediate energy photons (IEP) for stereotactic radiosurgery

    International Nuclear Information System (INIS)

    O'Malley, Lauren; Pignol, Jean-Philippe; Beachey, David J; Keller, Brian M; Presutti, Joseph; Sharpe, Michael

    2006-01-01

    Using efficient immobilization and dedicated beam collimation devices, stereotactic radiosurgery ensures highly conformal treatment of small tumours with limited microscopic extension. One contribution to normal tissue irradiation remains the radiological penumbra. This work aims at demonstrating that intermediate energy photons (IEP), above orthovoltage but below megavoltage, improve dose distribution for stereotactic radiosurgery for small irradiation field sizes due to a dramatic reduction of radiological penumbra. Two different simulation systems were used: (i) Monte Carlo simulation to investigate the dose distribution of monoenergetic IEP between 100 keV and 1 MeV in water phantom; (ii) the Pinnacle 3 TPS including a virtual IEP unit to investigate the dosimetry benefit of treating with 11 non-coplanar beams a 2 cm tumour in the middle of a brain adjacent to a 1 mm critical structure. Radiological penumbrae below 300 μm are generated for field size below 2 x 2 cm 2 using monoenergetic IEP beams between 200 and 400 keV. An 800 kV beam generated in a 0.5 mm tungsten target maximizes the photon intensity in this range. Pinnacle 3 confirms the dramatic reduction in penumbra size. DVHs show for a constant dose distribution conformality, improved dose distribution homogeneity and better sparing of critical structures using a 800 kV beam compared to a 6 MV beam

  16. Improvement of radiological penumbra using intermediate energy photons (IEP) for stereotactic radiosurgery

    Science.gov (United States)

    O'Malley, Lauren; Pignol, Jean-Philippe; Beachey, David J.; Keller, Brian M.; Presutti, Joseph; Sharpe, Michael

    2006-05-01

    Using efficient immobilization and dedicated beam collimation devices, stereotactic radiosurgery ensures highly conformal treatment of small tumours with limited microscopic extension. One contribution to normal tissue irradiation remains the radiological penumbra. This work aims at demonstrating that intermediate energy photons (IEP), above orthovoltage but below megavoltage, improve dose distribution for stereotactic radiosurgery for small irradiation field sizes due to a dramatic reduction of radiological penumbra. Two different simulation systems were used: (i) Monte Carlo simulation to investigate the dose distribution of monoenergetic IEP between 100 keV and 1 MeV in water phantom; (ii) the Pinnacle3 TPS including a virtual IEP unit to investigate the dosimetry benefit of treating with 11 non-coplanar beams a 2 cm tumour in the middle of a brain adjacent to a 1 mm critical structure. Radiological penumbrae below 300 µm are generated for field size below 2 × 2 cm2 using monoenergetic IEP beams between 200 and 400 keV. An 800 kV beam generated in a 0.5 mm tungsten target maximizes the photon intensity in this range. Pinnacle3 confirms the dramatic reduction in penumbra size. DVHs show for a constant dose distribution conformality, improved dose distribution homogeneity and better sparing of critical structures using a 800 kV beam compared to a 6 MV beam.

  17. Advancing Inclusive Mathematics Education: Strategies and Resources for Effective IEP Practices

    Science.gov (United States)

    Tan, Paulo

    2017-01-01

    Personal experiences promoting inclusive mathematics education for my own child have mostly been met with staunch resistance on the part of educators, and a resulting breakdown in collaborative efforts during individualized education program (IEP) meetings. However, I found that utilizing certain strategies and introducing innovative mathematics…

  18. Demystifying the IEP Process for Diverse Parents of Children with Disabilities

    Science.gov (United States)

    Lo, Lusa

    2012-01-01

    The complicated individualized education program (IEP) process and annual team meetings can be very stressful for families of children with disabilities, especially for those whose primary language is not English and those who are unfamiliar with the American special education system. Cultural and language barriers and lack of information…

  19. High School General Education English Teachers' Perception of IEP Accommodations for Students with Asperger Syndrome

    Science.gov (United States)

    Krones, Mary Patricia

    2016-01-01

    The purpose of this qualitative design study was to better understand the experiences of high school general education English teachers who have students with Asperger Syndrome in their classes. More specifically, this researcher wanted to better understand the teacher's perception of the IEP-denoted accommodations the general education teachers…

  20. Social Media Literacy as an IEP Intervention for Social and Emotional Learning

    Science.gov (United States)

    Probst, Donnell

    2017-01-01

    Media literacy and special education communities have largely ignored the impact of digital media useonspecial education students with Autism spectrum disorder and Emotional and Behavioral Disorder. This paper investigates the possibility of using social media literacy education as part of an individualized education plan (IEP) intervention for…

  1. An Investigation of Student Involvement in IEP Meetings in Suffolk County, New York

    Science.gov (United States)

    Jurgens, Matthew A.

    2016-01-01

    The purpose of this study was to identify the possible barriers as to why student involvement in IEP meetings has not been occurring in the past and to subsequently suggest further action and research as to how to potentially combat these barriers so students with disabilities in Suffolk County, Long Island can begin to be offered these kinds of…

  2. Six Things to Never Say or Hear during an IEP Meeting: Educators as Advocates for Families

    Science.gov (United States)

    Cheatham, Gregory A.; Hart, Juliet E.; Malian, Ida; McDonald, Joan

    2012-01-01

    The purpose of this paper is to support school professionals in their work with parents as they meet IDEA (2004) requirements during IEP meetings. The authors share tools to help recognize when IDEA principles are violated and provide alternative responses that school professionals and parents can offer. Definitions and brief explanations of the…

  3. Opinions of Prospective Classroom Teachers about Their Competence for Individualized Education Program (IEP)

    Science.gov (United States)

    Debbag, Murat

    2017-01-01

    This research aims to determine the opinions of prospective classroom teachers about preparation and implementation of Individualized Education Program (IEP). In this study, a qualitative research method was used. The participants were 20 classroom-teaching students that had been selected through the purposive sampling method. In the study, the…

  4. How Well Does Your IEP Measure Up?: Quality Indicators for Effective Service Delivery.

    Science.gov (United States)

    Twachtman-Cullen, Diane; Twachtman-Reilly, Jennifer

    This book is intended to offer guidance in writing individualized education programs (IEPs) that deliver high-quality, need-based educational programming for students with autism spectrum disorders. Following an introductory historical overview of special education law, the remaining chapters in part 1 address the quality indicators for each of…

  5. Dextrose-mediated aggregation of therapeutic monoclonal antibodies in human plasma: Implication of isoelectric precipitation of complement proteins.

    Science.gov (United States)

    Luo, Shen; Zhang, Baolin

    2015-01-01

    Many therapeutic monoclonal antibodies (mAbs) are clinically administered through intravenous infusion after mixing with a diluent, e.g., saline, 5% dextrose. Such a clinical setting increases the likelihood of interactions among mAb molecules, diluent, and plasma components, which may adversely affect product safety and efficacy. Avastin® (bevacizumab) and Herceptin® (trastuzumab), but not Remicade® (infliximab), were shown to undergo rapid aggregation upon dilution into 5% dextrose when mixed with human plasma in vitro; however, the biochemical pathways leading to the aggregation were not clearly defined. Here, we show that dextrose-mediated aggregation of Avastin or Herceptin in plasma involves isoelectric precipitation of complement proteins. Using mass spectrometry, we found that dextrose-induced insoluble aggregates were composed of mAb itself and multiple abundant plasma proteins, namely complement proteins C3, C4, factor H, fibronectin, and apolipoprotein. These plasma proteins, which are characterized by an isoelectronic point of 5.5-6.7, lost solubility at the resulting pH in the mixture with formulated Avastin (pH 6.2) and Herceptin (pH 6.0). Notably, switching formulation buffers for Avastin (pH 6.2) and Remicade (pH 7.2) reversed their aggregation profiles. Avastin formed little, if any, insoluble aggregates in dextrose-plasma upon raising the buffer pH to 7.2 or above. Furthermore, dextrose induced pH-dependent precipitation of plasma proteins, with massive insoluble aggregates being detected at pH 6.5-6.8. These data show that isoelectric precipitation of complement proteins is a prerequisite of dextrose-induced aggregation of mAb in human plasma. This finding highlights the importance of assessing the compatibility of a therapeutic mAb with diluent and human plasma during product development.

  6. Isoelectric focusing in continuously tapered fused silica capillary prepared by etching with supercritical water

    Czech Academy of Sciences Publication Activity Database

    Šlais, Karel; Horká, Marie; Karásek, Pavel; Planeta, Josef; Roth, Michal

    2013-01-01

    Roč. 85, č. 9 (2013), s. 4296-4300 ISSN 0003-2700 R&D Projects: GA ČR(CZ) GAP106/12/0522; GA MV VG20102015023 Institutional support: RVO:68081715 Keywords : capillary isoelectric focusing * resolution of ampholytes * supercritical water Subject RIV: CB - Analytical Chemistry, Separation Impact factor: 5.825, year: 2013

  7. Highly sensitive and specific protein detection via combined capillary isoelectric focusing and proximity ligation

    NARCIS (Netherlands)

    Padhan, N.; Yan, J.; Boge, A.; Scrivener, E.; Birgisson, H.; Zieba, A.; Gullberg, M.; Kamali-Moghaddam, M.; Claesson-Welsh, L.; Landegren, U.

    2017-01-01

    Detection and quantification of proteins and their post-translational modifications are crucial to decipher functions of complex protein networks in cell biology and medicine. Capillary isoelectric focusing together with antibody-based detection can resolve and identify proteins and their isoforms

  8. Analysis of recombinant proteins by isoelectric focusing in immobilized pH gradients

    NARCIS (Netherlands)

    Bischoff, Rainer; Roecklin, D.; Roitsch, C.

    1992-01-01

    Isoelectric focusing in immobilized pH gradients (IEF-IPG) was used to analyze three different recombinant proteins. Recombinant leech hirudin (65 amino acids, three disulfide bonds) expressed in Saccharomyces cerevisiae as a secreted protein and purified by anion-exchange and reversed-phase

  9. Commercial ampholytes used for isoelectric focusing may interfere with bioactivity based purification of antimicrobial peptides

    OpenAIRE

    Riazi, Shadi; Dover, Sara; Turovskiy, Yevgeniy; Chikindas, Michael L.

    2007-01-01

    BioRad's Rotofor® system has been frequently used for the purification of proteins and smaller peptides such as bacteriocins. In this study, we report that some commercially available ampholytes used with the Rotofor® isoelectric focusing system possess antimicrobial activity, which may interfere with the purification of bacteriocins and bacteriocin-like substances.

  10. Cortical neurons and networks are dormant but fully responsive during isoelectric brain state.

    Science.gov (United States)

    Altwegg-Boussac, Tristan; Schramm, Adrien E; Ballestero, Jimena; Grosselin, Fanny; Chavez, Mario; Lecas, Sarah; Baulac, Michel; Naccache, Lionel; Demeret, Sophie; Navarro, Vincent; Mahon, Séverine; Charpier, Stéphane

    2017-09-01

    A continuous isoelectric electroencephalogram reflects an interruption of endogenously-generated activity in cortical networks and systematically results in a complete dissolution of conscious processes. This electro-cerebral inactivity occurs during various brain disorders, including hypothermia, drug intoxication, long-lasting anoxia and brain trauma. It can also be induced in a therapeutic context, following the administration of high doses of barbiturate-derived compounds, to interrupt a hyper-refractory status epilepticus. Although altered sensory responses can be occasionally observed on an isoelectric electroencephalogram, the electrical membrane properties and synaptic responses of individual neurons during this cerebral state remain largely unknown. The aim of the present study was to characterize the intracellular correlates of a barbiturate-induced isoelectric electroencephalogram and to analyse the sensory-evoked synaptic responses that can emerge from a brain deprived of spontaneous electrical activity. We first examined the sensory responsiveness from patients suffering from intractable status epilepticus and treated by administration of thiopental. Multimodal sensory responses could be evoked on the flat electroencephalogram, including visually-evoked potentials that were significantly amplified and delayed, with a high trial-to-trial reproducibility compared to awake healthy subjects. Using an analogous pharmacological procedure to induce prolonged electro-cerebral inactivity in the rat, we could describe its cortical and subcortical intracellular counterparts. Neocortical, hippocampal and thalamo-cortical neurons were all silent during the isoelectric state and displayed a flat membrane potential significantly hyperpolarized compared with spontaneously active control states. Nonetheless, all recorded neurons could fire action potentials in response to intracellularly injected depolarizing current pulses and their specific intrinsic

  11. Quasi-isoelectric buffers for protein analysis in a fast alternative to conventional capillary zone electrophoresis.

    Science.gov (United States)

    Antonioli, Paolo; Mendieta, Martha E; Sebastiano, Roberto; Citterio, Attilio; Peltre, Gabriel; Busnel, Jean-Marc; Descroix, Stephanie; Candiano, Giovanni; Righetti, Pier Giorgio

    2006-03-20

    Two different approaches are here reported for obtaining ultra-narrow pI cuts from 2-pH unit wide carrier ampholyte ranges, as commercially available, for use as quasi-isoelectric buffers in capillary electrophoresis separations of proteins. One of them uses multicompartment electrolyzers endowed with isoelectric membranes (Immobiline technology); the other employs the Rotofor equipment. Although the first approach results in more precise pI cuts, the latter technique is much faster, easier to handle and permits the immediate collection of 20 fractions in a single run. This results in ultra-narrow, ca. 0.1-pH unit intervals, uniformly spaced apart along the original wider gradient utilized for the fractionation. It is here shown that such quasi-isoelectric buffers, especially those in the pH 8-9 interval, have the unique property of coating the silica wall, thus preventing interaction of the proteins with the silica surface, that would otherwise totally disrupt the separation. On the contrary, such a shielding is not obtained in control, non isoelectric buffers (such as phosphate), that give very poor separations in uncoated capillaries. It is hypothesized that such a unique shielding effect is due to the oligo-amino backbone of the carrier ampholytes, typically composed (in the Vesterberg's synthetic approach) of 4-6 nitrogens spaced apart by ethylene moieties. Although such oligoprotic buffers should bear, in the isoelectric state, just one positive and one negative charge, they might be transiently ionized upon contact with the silanols, thus inducing a cooperative binding to the silica wall.

  12. Divergent flow isoelectric focusing: fast and efficient method for protein sample preparation for mass spectrometry

    Czech Academy of Sciences Publication Activity Database

    Mazanec, Karel; Bobálová, Janette; Šlais, Karel

    2009-01-01

    Roč. 393, 6-7 (2009), s. 1769-1778 ISSN 1618-2642 R&D Projects: GA MŠk 1M0570; GA AV ČR IAAX00310701 Institutional research plan: CEZ:AV0Z40310501 Keywords : Isoelectric focusing * proteins * MALDI-TOF/TOF MS Subject RIV: CB - Analytical Chemistry, Separation Impact factor: 3.480, year: 2009

  13. Analysis of glycated hemoglobin A1c by capillary electrophoresis and capillary isoelectric focusing

    Czech Academy of Sciences Publication Activity Database

    Koval, Dušan; Kašička, Václav; Cottet, H.

    2011-01-01

    Roč. 413, č. 1 (2011), s. 8-15 ISSN 0003-2697 R&D Projects: GA ČR GP203/09/P485; GA ČR(CZ) GA203/08/1428 Institutional research plan: CEZ:AV0Z40550506 Keywords : capillary zone electrophoresis * capillary isoelectric focusing * glycated hemoglobin HbA1c Subject RIV: CB - Analytical Chemistry, Separation Impact factor: 2.996, year: 2011

  14. Molecular Design of Low-Density Multifunctional Hybrid Materials

    Science.gov (United States)

    2016-01-01

    scale inversely with the isoelectric point ( IEP ) of the underlying surface (Fig. 19). If the IEP of the substrate surface (e.g. metal oxide) or sol...gel solution ion is less than the solution pH, the surface will take on a net negative charge; conversely, for IEP values greater than the solution pH...as a function of substrate IEP . 1 2 3 4 5 6 7 8 9 10 11 0 10 20 30 40 50 60 70 C ri ti c a l F ra c tu re E n e rg y , G C ( J m -2

  15. Are We Practicing What We Are Preaching? An Evaluation of Self-Determination Instructional Components in IEPs at a Midwestern High School

    Science.gov (United States)

    Lopez, Nancy; Stoner, Julia B.

    2017-01-01

    Promoting self-determination in students with disabilities is recognized as an integral component of education today. For most students with disabilities, self-determination should be addressed through instruction and in the Individualized Education Program (IEP). Yet, IEPs are rarely evaluated to determine if goals targeting self-determination…

  16. Comparison of isoelectric focusing and polymerase chain reaction for the detection of β-lactamases

    Directory of Open Access Journals (Sweden)

    Sharma J

    2010-01-01

    Full Text Available Extended spectrum β-lactamases (ESBLs have been observed in virtually all the species of family Enterobacteriaceae. Threat posed by antibiotic resistance because of ESBLs is more serious as a number of technical problems are associated with the detection of these enzymes. Although a number of detection methods have been designed for ESBLs, every method has its own benefits and shortcomings as well. In earlier days, isoelectric focusing (IEF was used as the gold standard for ESBL detection. This study was undertaken to compare IEF with polymerase chain reaction, a method which has been extensively used for ESBL detection these days.

  17. From Image Processing to Classification: 1. Modelling Disturbances of Isoelectric Focusing Patterns

    DEFF Research Database (Denmark)

    Jensen, Karsten; Søndergaard, I.; Skovgaard, I. M.

    1995-01-01

    In order to optimize the conditions for evaluation of isoelectric focusing (IEF) patterns by digital image processing, the sources of error in determination of the pi values were analyzed together with the influence of a varying background. The effects of band distortions, in the spectra...... of the individual lanes, were examined. In order to minimize the effect of these distortions, optimal conditions for handling IEF patterns by digital image processing were elucidated. The systematic part of the global deformation on the gels was investigated and an algorithm was developed by which it was possible...

  18. Carrier ampholyte-free isoelectric focusing on a paper-based analytical device for the fractionation of proteins.

    Science.gov (United States)

    Xie, Song-Fang; Gao, Han; Niu, Li-Li; Xie, Zhen-Sheng; Fang, Fang; Wu, Zhi-Yong; Yang, Fu-Quan

    2018-01-25

    Isoelectric focusing plays a critical role in the analysis of complex protein samples. Conventionally, isoelectric focusing is implemented with carrier ampholytes in capillary or immobilized pH gradient gel. In this study, we successfully exhibited a carrier ampholyte-free isoelectric focusing on paper-based analytical device. Proof of the concept was visually demonstrated with color model proteins. Experimental results showed that not only a pH gradient was well established along the open paper fluidic channel as confirmed by pH indicator strip, the pH gradient range could also be tuned by the catholyte or anolyte. Furthermore, the isoelectric focusing fractions from the paper channel can be directly cut and recovered into solutions for post analysis with sodium dodecyl sulfate-polyacrylamide gel electrophoresis and matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry. This paper-based isoelectric focusing method is fast, cheap, simple and easy to operate, and could potentially be used as a cost-effective protein sample clean-up method for target protein analysis with mass spectrometry. © 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  19. Gc protein-derived macrophage activating factor (GcMAF): isoelectric focusing pattern and tumoricidal activity.

    Science.gov (United States)

    Mohamad, Saharuddin Bin; Nagasawa, Hideko; Sasaki, Hideyuki; Uto, Yoshihiro; Nakagawa, Yoshinori; Kawashima, Ken; Hori, Hitoshi

    2003-01-01

    Gc protein is the precursor for Gc protein-derived macrophage activating factor (GcMAF), with three phenotypes: Gc1f, Gc1s and Gc2, based on its electrophoretic mobility. The difference in electrophoretic mobility is because of the difference in its posttranslational sugar moiety composition. We compared the difference between Gc protein and GcMAF electrophoretic mobility using the isoelectric focusing (IEF) method. The tumoricidal activity of GcMAF-treated macrophage was evaluated after coculture with L-929 cell. The tumoricidal mechanism was investigated using TNF bioassay and nitric oxide (NO) release. The difference in Gc protein and GcMAF electrophoretic mobility was detected. The tumoricidal activity of GcMAF-treated macrophage was detected, but no release of TNF and NO was detected. The difference of isoelectric focusing mobility in Gc protein and GcMAF would be useful to develop a GcMAF detection method. GcMAF increased macrophage tumoricidal activity but TNF and NO release were not involved in the mechanism.

  20. Can Appreciative Inquiry Increase Positive Interactions, Student Self-Advocacy and Turn-Taking during IEP Meetings?

    Science.gov (United States)

    Kozik, Peter L.

    2018-01-01

    This comparative research study in the context of action research documents the effects of Appreciative Inquiry (AI) on positive participant interactions, student turn-taking and self-advocacy interactions during IEP meetings that focused on student transition to post-secondary outcomes. AI was implemented as a written protocol for conducting IEP…

  1. Has Technology Been Considered? A Guide for IEP Teams. CASE/TAM Assistive Technology Policy and Practice Series.

    Science.gov (United States)

    Chambers, A. C.

    This guide compiles information essential to a working knowledge of assistive technology for children with disabilities. It addresses the definition of assistive technology and provides information on laws which direct the provision of assistive technology. The manual provides a framework to guide the Individualized Education Program (IEP) team as…

  2. Enhancing Recognition of High Quality, Functional IEP Goals: A Training Activity for Early Childhood Special Education Staff

    Science.gov (United States)

    Lucas, Anne; Gillaspy, Kathi; Peters, Mary Louise; Hurth, Joicey

    2014-01-01

    This training activity was created to support participants' understanding of the criteria needed to develop and write high quality, participation-based Individualized Education Program (IEP) goals. The term "functional" is often used to describe what goals ought to be, yet many Early Childhood Special Education (ECSE) staff (e.g.,…

  3. In the Best Interests of the Child: Individualized Education Program (IEP) Meetings When Parents Are in Conflict

    Science.gov (United States)

    Feinberg, Edward; Moses, Philip; Engiles, Anita; Whitehorne, Amy; Peter, Marshall

    2014-01-01

    Individual Education Program (IEP) teams are composed of diverse individuals, each bringing a unique set of experiences, knowledge, and skills to the table. Given this, it is not uncommon for team members to have different views on the special education and related services needs of the student whose plan is being developed. When divergent views…

  4. The Use of a Rubric as a Tool to Guide Pre-Service Teachers in the Development of IEPs

    Science.gov (United States)

    Rosas, Clarissa E.; Winterman, Kathleen G.

    2012-01-01

    The challenge of developing Individualized Education Program documents that are representative of a team decision making process and are in compliance with IDEA 2004 is well documented in the literature. One of the main objectives of IEPs is to serve as the foundation of a child's academic program. Inclusion of children with disabilities in the…

  5. Effects of School Counselor Supervised Peer Tutoring in Inclusive Settings on Meeting IEP Outcomes of Students with Developmental Disabilities

    Science.gov (United States)

    Odluyurt, Serhat; Tekin-Iftar, Elif; Ersoy, Gulhan

    2014-01-01

    The purpose of this study was to investigate the effects of school counselor supervised peer tutoring intervention on meeting IEP outcomes of six inclusion students with developmental disabilities in a public elementary and secondary school. The effectiveness of this intervention was evaluated by using multiple probe design across students.…

  6. Using isoelectric focussing and neutron activation analysis to study protein-bound tracer elements

    International Nuclear Information System (INIS)

    Schmelzer, W.

    1976-01-01

    A method to determine protein-bound tracer elements was determined by combining a protein separation method with neutron activation analysis. Gel filtration, disk electrophoresis, and isoelectric focussing were studied with regard to their suitability as separation methods. Using isoelectric focussing, human serum protein could be separated with good resolution on a preparative scale. The Se, Cr, Ag, Sc, Fe, Zn, Co, Br, Na, Rb, and Cs contents of the various protein fractions were determined by instrumental neutron activation analysis and by gamma-spectroscopic identification of their long-lived nuclides. Particular attention was paid to the main source of error with this method, i.e. contamination of the proteins in the course of the separation process. Information on the binding of the elements to protein was obtained by comparing the contents in the serum and in the protein separated by gel chromatography. For example, 75% of the Se and 30% of the Cs are bound to protein. Contamination of the protein fractions was studied by means of tracer elements with the element Se, errors due to contamination could be ruled out. The method was modified for the special imvestigation of Se-protein complexes in the serum. The Se content was determined by activation analysis via the short-lived radionuclide sup(77m)Se, this considerably reducing the duration of analysis. With regard to focussing, discrimination was improved in the pH region in which specific Se complexes were found. The activity distribution in fractionated serum protein labelled in vitro with 75 Se in the presence of erythrocytes showed that specific labelling is possible in this way. It is thus possible to study the distribution of Se carrier proteins with the aid of a radiotracer technique. (orig./RB) [de

  7. A three-prong strategy to develop functional food using protein isolates recovered from chicken processing by-products with isoelectric solubilization/precipitation.

    Science.gov (United States)

    Tahergorabi, Reza; Sivanandan, Litha; Beamer, Sarah K; Matak, Kristen E; Jaczynski, Jacek

    2012-09-01

    Skin-on bone-in chicken drumsticks were processed with isoelectric solubilization/precipitation to recover muscle proteins. The drumsticks were used as a model for dark chicken meat processing by-products. The main objective of this study was conversion of dark chicken meat processing by-products to restructured functional food product. An attempt was made to develop functional food product that would resemble respective product made from boneless skinless chicken breast meat. A three-prong strategy to address diet-driven cardiovascular disease (CVD)with a functional food was used in this study. The strategy included addition of three ingredients with well-documented cardiovascular benefits: (i) ω-3 polyunsaturated fatty acid-rich oil (flaxseed-algae, 9:1); (ii) soluble fiber; and (iii) salt substitute. Titanium dioxide, potato starch, polyphosphate, and transglutaminase were also added. The batters were formulated and cooked resulting in heat-set gels. Color (L*a*b*), texture (torsion test, Kramer shear test, and texture profile analysis), thermal denaturation (differential scanning calorimetry), and gelation (dynamic rheology) of chicken drumstick gels and chicken breast gels were determined and compared. Chicken drumstick gels generally had comparable color and texture properties to the gels made from chicken breast meat. The endothermic transition (thermal denaturation) of myosin was more pronounced and gelation properties were better for the drumstick gels. This study demonstrated a feasibility to develop functional food made of muscle proteins recovered with isoelectric solubilization/precipitation from low-value dark chicken meat processing by-products. The functional food developed in this study was enriched with CVD-beneficial nutrients and had comparable instrumental quality attributes to respective products made of chicken breast meat. Although the results of this study point towards the potential for a novel, marketable functional food product, sensory

  8. Reduction in Friction and Wear of Alumina Surfaces as Assisted with Surface-Adsorbing Polymers in Aqueous Solutions

    DEFF Research Database (Denmark)

    Røn, Troels; Lee, Seunghwan

    2016-01-01

    We have investigated the aqueous lubricating effects of various polymers for the sliding contacts of self-mated alumina surfaces in neutral aqueous environment. Given that isoelectric point (IEP) of alumina is ca. pH 9, polyanions can readily adsorb onto alumina surface at neutral pH via electros......We have investigated the aqueous lubricating effects of various polymers for the sliding contacts of self-mated alumina surfaces in neutral aqueous environment. Given that isoelectric point (IEP) of alumina is ca. pH 9, polyanions can readily adsorb onto alumina surface at neutral pH via...

  9. The trace analysis of microorganisms in real samples by combination of a filtration microcartridge and capillary isoelectric focusing

    Czech Academy of Sciences Publication Activity Database

    Horká, Marie; Horký, J.; Kubesová, Anna; Zapletalová, E.; Šlais, Karel

    2011-01-01

    Roč. 400, č. 9 (2011), s. 3133-3140 ISSN 1618-2642 R&D Projects: GA AV ČR IAAX00310701 Institutional research plan: CEZ:AV0Z40310501 Keywords : capillary isoelectric focusing * real samples * spores * fungi Subject RIV: CB - Analytical Chemistry, Separation Impact factor: 3.778, year: 2011

  10. Isoelectric point is an inadequate descriptor of MS2, Phi X 174 and PRD1 phages adhesion on abiotic surfaces.

    Science.gov (United States)

    Dika, Christelle; Duval, Jérôme F L; Francius, Gregory; Perrin, Aline; Gantzer, Christophe

    2015-05-15

    MS2, Phi X 174 and PRD1 bacteriophages are commonly used as surrogates to evaluate pathogenic virus behavior in natural aquatic media. The interfacial properties of these model soft bioparticles are herein discussed in connection with their propensities to adhere onto abiotic surfaces that differ in terms of surface charges and hydrophobicities. The phages considered in this work exhibit distinct multilayered surface structures and their electrostatic charges are evaluated from the dependence of their electrophoretic mobilities on electrolyte concentration at neutral pH on the basis of electrokinetic theory for soft (bio)particles. The charges of the viruses probed by electrokinetics vary according to the sequence Phi X 174⩽PRD1≪MS2, where 'Phi X 174≪MS2Phi X 174 and PRD1 bacteriophages. Copyright © 2014 Elsevier Inc. All rights reserved.

  11. Inteligência emocional percebida (IEP ajuste emocional e actividade física na terceira idade

    Directory of Open Access Journals (Sweden)

    Maria Manuela Lima de Figueiredo Queirós

    2007-06-01

    Full Text Available O interesse deste estudo está centrado na forma como interage a inteligência emocional percebida (IEP com a prática da actividade física e que peso específi co tem cada uma das variáveis estudadas sobre o ajuste emocional das pessoas nesta faixa etária e, consequentemente, na sua qualidade de vida.

  12. Optically directed molecular transport and 3D isoelectric positioning of amphoteric biomolecules

    International Nuclear Information System (INIS)

    Hafeman, Dean G.; Harkins, James B.; Witkowski, Charles E. II; Lewis, Nathan S.; Brown, Gilbert M.; Warmack, Robert J. Bruce; Thundat, Thomas George

    2006-01-01

    We demonstrate the formation of charged molecular packets and their transport within optically created electrical force-field traps in a pH-buffered electrolyte. We call this process photoelectrophoretic localization and transport (PELT). The electrolyte is in contact with a photoconductive semiconductor electrode and a counterelectrode that are connected through an external circuit. A light beam directed to coordinates on the photoconductive electrode surface produces a photocurrent within the circuit and electrolyte. Within the electrolyte, the photocurrent creates localized force-field traps centered at the illuminated coordinates. Charged molecules, including polypeptides and proteins, electrophoretically accumulate into the traps and subsequently can be transported in the electrolyte by moving the traps over the photoconductive electrode in response to movement of the light beam. The molecules in a single trap can be divided into aliquots, and the aliquots can be directed along multiple routes simultaneously by using multiple light beams. This photoelectrophoretic transport of charged molecules by PELT resembles the electrostatic transport of electrons within force-field wells of solid-state charge-coupled devices. The molecules, however, travel in a liquid electrolyte rather than a solid. Furthermore, we have used PELT to position amphoteric biomolecules in three dimensions. A 3D pH gradient was created in an electrolyte medium by controlling the illumination position on a photoconductive anode where protons were generated electrolytically. Photoelectrophoretic transport of amphoteric molecules through the pH gradient resulted in accumulation of the molecules at their apparent 3D isoelectric coordinates in the medium.

  13. ISOELECTRIC FOCUSING OF MEMBRANE PROTEINS OF PROBIOTIC B. COAGULANS AND ITS BACTERIOPHAGE RESISTANT MUTANTS

    Directory of Open Access Journals (Sweden)

    Kavita Rajesh Pandey

    2016-09-01

    Full Text Available Bacteriophages are the most notorious type of infection in the probiotic and dairy fermentations. Two phage resistant mutants viz. B. co PIII and B. co MIII (B. coagulans mutants PIII and MIII obtained in previous studies (Dubey and Vakil, 2010, were further characterized for their protein profile in comparison with the parental probiotic strain –B. coagulans. The cell lysates were subjected to ultra-centrifugation and the purified membrane fractions were resolved using 2D gel electrophoresis. The Isoelectric focussing showed 187, 202 and 154 protein spots for the parental strain, mutant B. co PIII and mutant B. co MIII, respectively. Ten and 18 protein spots were missing as compared to parent for mutants B.co PIII and B.co MIII whereas there were 21 and 14 new spots noticed for these two mutants. Eight membrane proteins present only in the phage sensitive parental culture could be tentatively identified by comparison with the complete proteome of B. coagulans by use of UniprotKB and then CELLO database It is quite likely that some of these identified membrane proteins may be also functioning as receptors for phage adsorption followed by entry of nucleic acid into the phage sensitive host cell.

  14. Detection of ST-T Episode Based on the Global Curvature of Isoelectric Level in ECG

    Energy Technology Data Exchange (ETDEWEB)

    Kang, D. W.; Jun, D. G.; Lee, K. J.; Yoon, H. R. [Yonsei University, Seoul (Korea)

    2001-04-01

    This paper describes an automated detection algorithm of ST-T episodes using global curvature which can connect the isoelectric level in ECG and can eliminate not only the slope of ST segment, but also difference of the baseline and global curve. This above method of baseline correction is very faster than classical baseline correction methods. The optimal values of parameters for baseline correction were found as the value having the highest detection rate of ST episode. The features as input of backpropagation Neural Network were extracted from the whole ST segment. The European ST-T database was used as training and test data. Finally, ST elevation, ST depression and normal ST were classified. The average ST episode sensitivity and predictivity were 85.42%, 80.29%, respectively. This result shows the high speed and reliability in ST episode detection. In conclusion, the proposed method showed the possibility in various applications for the Holter system. (author). 17 refs., 5 figs., 4 tabs.

  15. Use of quasi-isoelectric buffers to limit protein adsorption in capillary zone electrophoresis.

    Science.gov (United States)

    Poitevin, Martine; Hammad, Karim; Ayed, Ichraf; Righetti, Pier Giorgio; Peltre, Gabriel; Descroix, Stephanie

    2008-08-01

    The use of quasi-isoelectric buffers consisting of narrow pH cuts of carrier ampholytes (NC) has been investigated to limit protein adsorption on capillary walls during capillary zone electrophoresis experiments. To quantify protein adsorption on the silica surface, a method derived from that of Towns and Regnier has been developed. alpha-Lactalbumin (14 kDa, pI 4.8) and alpha-chymotrypsinogen A (25 kDa, pI 9.2) have been used as model proteins. Acidic narrow pH cuts of carrier ampholytes (NC, pH 3.0) obtained from fractionation of Serva 4-9 carrier ampholytes were used as BGE in bare-silica capillaries, and allowed to decrease significantly protein adsorption, as compared to experiments performed with classical formate buffer. The use of NC as BGE appeared to be as efficient as the use of polydimethylacrylamide coating to prevent protein adsorption. This increase of protein recovery when using NC was attributed to the interaction of carrier ampholytes with the silica surface, leading to a shielding of the capillary wall.

  16. Performing Isoelectric Focusing and Simultaneous Fractionation of Proteins on A Rotary Valve Followed by Sodium Dodecyl – Polyacrylamide Gel Electrophoresis

    Science.gov (United States)

    Wang, Wei; Lu, Joann J.; Gu, Congying; Zhou, Lei; Liu, Shaorong

    2013-01-01

    In this technical note, we design and fabricate a novel rotary valve and demonstrate its feasibility for performing isoelectric focusing and simultaneous fractionation of proteins, followed by sodium dodecyl – polyacrylamide gel electrophoresis. The valve has two positions. In one position, the valve routes a series of capillary loops together into a single capillary tube where capillary isoelectric focusing (CIEF) is performed. By switching the valve to another position, the CIEF-resolved proteins in all capillary loops are isolated simultaneously, and samples in the loops are removed and collected in vials. After the collected samples are briefly processed, they are separated via sodium dodecyl – polyacrylamide gel electrophoresis (SDS-PAGE, the 2nd-D separation) on either a capillary gel electrophoresis instrument or a slab-gel system. The detailed valve configuration is illustrated, and the experimental conditions and operation protocols are discussed. PMID:23819755

  17. Diagnosis of common hemoglobinopathies among South East Asian population using capillary isoelectric focusing system.

    Science.gov (United States)

    Srivorakun, H; Fucharoen, G; Sanchaisuriya, K; Fucharoen, S

    2017-02-01

    We have evaluated an automated capillary isoelectric focusing (cIEF)-based Hb analyzer in diagnosis of hemoglobinopathies commonly found among South East Asian population. Study was performed on a cohort of 665 adult Thai subjects and 13 fetal blood specimens obtained at routine thalassemia diagnostic laboratory. Hb analysis was performed using the cIEF system. Thalassemia genotypes were defined by DNA analysis. The system revealed satisfactorily within-run and between-run precision for quantitation of Hb A 2 and Hb E (CV: 0.02-0.09%). The reference ranges of Hb A 2 and Hb E were 2.6-4.0% and 25.7-33.1%, respectively. The system identified the cases of β-thalassemia and Hb E disorders correctly. Several thalassemia genotypes and Hb variants were identifiable. However, Hb Constant Spring was separated closely to Hb A 2 and Hbs Bart's and H were relatively difficult to be reported due to interfering peaks separating at the same regions. Prenatal diagnosis by fetal blood analysis was found to be accurate for Hb Bart's hydrops fetalis and Hb E-β 0 -thalassemia disease. The cIEF system could accurately diagnose β-thalassemia and Hb E carriers and demonstrate many Hb variants found in the region. The system cannot report Hb A 2 in the presence of Hb E whereas Hbs Lepore and F are comigrated. Diagnosis of α-thalassemia disease based on Hb H and Hb Bart's might be difficult. © 2016 John Wiley & Sons Ltd.

  18. OFFGEL isoelectric focusing and polyacrylamide gel electrophoresis separation of platinum-binding proteins.

    Science.gov (United States)

    Mena, Ma Luz; Moreno-Gordaliza, Estefanía; Moraleja, Irene; Cañas, Benito; Gómez-Gómez, Ma Milagros

    2011-03-04

    In this work a 2D electrophoretic separation procedure able to maintain the integrity of platinum-protein bonds has been developed. The method is based on the use of sequential OFFGEL isoelectric focussing (IEF) and PAGE. A systematic study of the reagents used for PAGE, for OFFGEL-IEF separation, and post-separation treatment of gels (such as enzymatic digestion and sample preparation for MS analysis) was tackled regarding their suitability for the identification of platinum binding proteins using standard proteins incubated with cisplatin. The distribution of platinum in high and low molecular weight fractions (separated by cut-off filters) was determined by ICP-MS, which allows evaluating platinum-protein bond stability under the conditions studied. SDS-PAGE in the absence of β-mercaptoethanol or dithiotreitol preserved the platinum-protein bonds. In addition, neither the influence of the electric field during the electrophoretic separation, nor the processes of fixing, staining and destaining of proteins in the gel did result in the loss of platinum from platinum binding proteins. SDS-PAGE under non-reducing conditions provides separation of platinum-binding proteins in very narrow bands with quantitative recoveries. Different amounts of platinum-bound proteins covering the range 0.3-2.0 μg were separated and mineralised for platinum determination, showing good platinum linearity. Limits of detection for a mixture of five standard proteins incubated with cisplatin were between the range of 2.4 and 13.9 pg of platinum, which were satisfactory for their application to biological samples. Regarding OFFGEL-IEF, a denaturing solution without thiourea and without dithiotreitol is recommended. The suitability of the OFFGEL-IEF for the separation of platinum binding proteins of a kidney cytosol was demonstrated. Copyright © 2011 Elsevier B.V. All rights reserved.

  19. Torsades de Pointes

    Directory of Open Access Journals (Sweden)

    Richard J Chen, MD

    2018-04-01

    Full Text Available History of present illness: 70-year-old male with a history ventricular arrhythmia, AICD (automated implantable cardioverter defibrillator, coronary artery disease and cardiac stents presented to the Emergency Department after three AICD discharges with dyspnea but no chest pain. During triage, he was found to have an irregular radial pulse and was placed on a cardiac monitor. Significant findings: The patient was found to be in a polymorphic ventricular tachycardia; he was alert, awake and asymptomatic. A rhythm strip showed a wide complex tachycardia with the QRS complex varying in amplitude around the isoelectric line consistent with Torsades de Pointes. Discussion: Torsades de Pointes (TdP is a specific type of polymorphic ventricular tachycardia. The arrhythmia’s characteristic morphology consists of the QRS complex “twisting” around the isoelectric line with gradual variation of the amplitude, reflecting its literal translation of “twisting of the points.”1 This arrhythmia occurs in the context of prolonged QT. The most common form of acquired QT prolongation is medication induced. Common causes include antiarrhythmics, antipsychotics, antiemetics, and antibiotics.2 Patient specific risk factors include female sex, bradycardia, hypokalemia, hypomagnesemia, hypocalcemia, hypothermia and heart disease.3 In the setting of prolonged QT, the repolarization phase is extended. TdP is initiated when a PVC (premature ventricular contraction occurs during this repolarization, known as an ‘R on T’ phenomenon. TdP is often asymptomatic and self-limited. The danger in TdP is its potential to deteriorate into ventricular fibrillation. A mainstay of management of TdP is prevention of risk factors when possible.4 Unstable patients should be treated with synchronized cardioversion. Magnesium sulfate should be administered in all cases of TdP.1 If a patient is not responsive to magnesium, consider isoproterenol, amiodarone, and overdrive

  20. Recycling isoelectric focusing with computer controlled data acquisition system. [for high resolution electrophoretic separation and purification of biomolecules

    Science.gov (United States)

    Egen, N. B.; Twitty, G. E.; Bier, M.

    1979-01-01

    Isoelectric focusing is a high-resolution technique for separating and purifying large peptides, proteins, and other biomolecules. The apparatus described in the present paper constitutes a new approach to fluid stabilization and increased throughput. Stabilization is achieved by flowing the process fluid uniformly through an array of closely spaced filter elements oriented parallel both to the electrodes and the direction of the flow. This seems to overcome the major difficulties of parabolic flow and electroosmosis at the walls, while limiting the convection to chamber compartments defined by adjacent spacers. Increased throughput is achieved by recirculating the process fluid through external heat exchange reservoirs, where the Joule heat is dissipated.

  1. High-resolution slab gel isoelectric focusing: methods for quantitative electrophoretic transfer and immunodetection of proteins as applied to the study of the multiple isoelectric forms of ornithine decarboxylase.

    Science.gov (United States)

    Reddy, S G; Cochran, B J; Worth, L L; Knutson, V P; Haddox, M K

    1994-04-01

    A high-resolution isoelectric focusing vertical slab gel method which can resolve proteins which differ by a single charge was developed and this method was applied to the study of the multiple isoelectric forms of ornithine decarboxylase. Separation of proteins at this high level of resolution was achieved by increasing the ampholyte concentration in the gels to 6%. Various lots of ampholytes, from the same or different commercial sources, differed significantly in their protein binding capacity. Ampholytes bound to proteins interfered both with the electrophoretic transfer of proteins from the gel to immunoblotting membranes and with the ability of antibodies to interact with proteins on the immunoblotting membranes. Increasing the amount of protein loaded into a gel lane also decreased the efficiency of the electrophoretic transfer and immunodetection. To overcome these problems, both gel washing and gel electrophoretic transfer protocols for disrupting the ampholyte-protein binding and enabling a quantitative electrophoretic transfer of proteins were developed. Two gel washing procedures, with either thiocyanate or borate buffers, and a two-step electrophoretic transfer method are described. The choice of which method to use to optimally disrupt the ampholyte-protein binding was found to vary with each lot of ampholytes employed.

  2. Effects of the CD-Rom Version of the "Self-Advocacy Strategy" on Quality of Contributions in IEP Meetings of High School Students with Intellectual Disability

    Science.gov (United States)

    Cease-Cook, Jennifer; Test, David W.; Scroggins, La' Shawndra

    2013-01-01

    This study used a multiple-probe across participants design to examine the effects of the CD-Rom version of the "Self-Advocacy Strategy" on quality of contributions in Individual Education Plan (IEP) meetings of five high school students with intellectual disability. Results indicated a functional relationship between using the CD-Rom…

  3. Creating a "Win-Win IEP" for Students with Autism: A How-To Manual for Parents and Educators. Second Edition.

    Science.gov (United States)

    Fouse, Beth

    This book is intended to provide parents of students with autism with necessary information for the Individualized Education Program (IEP) process and includes relevant information from the 1997 Individuals with Disabilities Education Act. Chapters discuss the following topics: (1) introductory information; (2) definitions of autism, the basic…

  4. Comparison of Transition-Related IEP Content for Young Adults with Disabilities Who Do or Do Not Have a Legal Guardian

    Science.gov (United States)

    Millar, Dorothy Squatrito

    2009-01-01

    IEP transition-related content was compared between young adults with developmental disabilities who had or did not have legal guardians. It was found that students with guardians were more likely to earn a certificate of completion, and wanted to remain living with their families, in comparison to students without guardians who were more likely…

  5. Motor control and cardiovascular responses during isoelectric contractions of the upper trapezius muscle: evidence for individual adaptation strategies.

    Science.gov (United States)

    Mathiassen, S E; Aminoff, T

    1997-01-01

    Ten females (25-50 years of age) performed isometric shoulder flexions, holding the right arm straight and in a horizontal position. The subjects were able to see the rectified surface electromyogram (EMG) from either one of two electrode pairs above the upper trapezius muscle and were instructed to keep its amplitude constant for 15 min while gradually unloading the arm against a support. The EMG electrodes were placed at positions representing a "cranial" and a "caudal" region of the muscle suggested previously to possess different functional properties. During the two contractions, recordings were made of: (1) EMG root mean square-amplitude and zero crossing (ZC) frequency from both electrode pairs on the trapezius as well as from the anterior part of the deltoideus, (2) supportive force, (3) heart rate (HR) and mean arterial blood pressure (MAP), and (4) perceived fatigue. The median responses during the cranial isoelectric contraction were small as compared to those reported previously in the literature: changes in exerted glenohumeral torque and ZC rate of the isoelectric EMG signal of -2.81% x min(-1) (P = 0.003) and 0.03% x min(-1) (P = 0.54), respectively, and increases in HR and MAP of 0.14 beats x min(-2) (P = 0.10) and 0.06 mmHg x min(-1) (P = 0.33), respectively. During the contraction with constant caudal EMG amplitude, the corresponding median responses were -2.51% x min(-1) (torque), 0.01% x min(-1) (ZC rate), 0.31 beats x min(-2) (HR), and 0.93 mmHg x min(-1) (MAP); P = 0.001, 0.69, 0.005, and 0.003, respectively. Considerable deviations from the "isoelectric" target amplitude were common for both contractions. Individuals differed markedly in response, and three distinct subgroups of subjects were identified using cluster analysis. These groups are suggested to represent different motor control scenarios, including differential engagement of subdivisions of the upper trapezius, alternating motor unit recruitment and, in one group, a gradual

  6. Studies on the oligosaccharide heterogeneity of the isoelectric forms of the lower molecular weight acid phosphatase of frog liver.

    Science.gov (United States)

    Kubicz, A; Szalewicz, A; Chrambach, A

    1991-01-01

    1. The lower molecular weight, heterogeneous acid phosphatase (AcPase) from the frog liver (Rana esculenta) containing AcPase I, II, III and IV was separated into enzymatically active components by isoelectric focusing in an immobilized pH gradient. 2. The blotted enzyme bands were characterized by their different binding patterns obtained with the lectins concanavalin A, wheat germ agglutinin (WGA), Lens culinaris hemagglutinin (LcH) and peanut agglutinin (PNA). 3. In situ neuraminidase treatment reduced the staining intensity of some WGA-bands and increased that of PNA-bands. 4. The finding that AcPases I, II, III and IV differ in their carbohydrate chain composition, together with previous results showing different bioactivities of AcPases III and IV, indicates a correlation between the glycosylation state of enzyme forms and their physiological action.

  7. Electrokinetic behavior of oxide components in Y(La)-Ba-Cu-O systems

    International Nuclear Information System (INIS)

    Chia, Y.H.

    1988-01-01

    Surface charge characterization of Y 2 O 3 , La 2 O 3 , CuO, and BaCO 3 was performed using a colloid vibration potential method. The effect of inorganic electrolyte, dissolved ions of other oxides, particle concentration, and mixing time on the pH of the isoelectric point (IEP) was investigated. The IEP of these oxides will be compared with the points of zero charge (PZC) obtained from the calculated pH of the minimum solubility of the solids and also with the reported PZC predicted by using the modified Parks' equation

  8. Micro-emulsification/encapsulation of krill oil by complex coacervation with krill protein isolated using isoelectric solubilization/precipitation.

    Science.gov (United States)

    Shi, Liu; Beamer, Sarah K; Yang, Hong; Jaczynski, Jacek

    2018-04-01

    This study determined feasibility of krill protein isolated with isoelectric solubilization/precipitation (ISP) as wall material to microencapsulate krill oil by freeze-drying. Effects of krill oil/krill protein ratio on properties of microcapsules were investigated. With increased ratio, crude protein of microcapsules decreased, while total lipid increased. Although microcapsule oil loading capacity increased, loading and encapsulation efficiencies decreased. Thin layer chromatography (TLC) confirmed abundance of phospholipids, which are amphiphilic; and thus, resulted in stable emulsion (emulsion stability index). Microcapsules contained ω-3 polyunsaturated fatty acids (PUFAs) at 43-60, including eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) at 28-41 and 9-11 g/100g of total FAs, respectively. SDS-PAGE electrophoresis revealed proteolysis of ISP krill protein, probably causing reduced loading and encapsulation efficiencies. SEM showed that krill oil/krill protein ratio affected surface microstructure. ISP krill protein showed potential as a wall material to microencapsulate krill oil; and thus, expand application of krill oil/protein for human consumption. Copyright © 2017 Elsevier Ltd. All rights reserved.

  9. A simple method for preparation of macroporous polydimethylsiloxane membrane for microfluidic chip-based isoelectric focusing applications

    Energy Technology Data Exchange (ETDEWEB)

    Ou Junjie [Department of Mechanical and Mechatronics Engineering, University of Waterloo, 200 University Ave West, Waterloo, Ontario, N2L 3G1 (Canada); Department of Chemistry, University of Waterloo, 200 University Ave West, Waterloo, Ontario, N2L 3G1 (Canada); Ren, Carolyn L., E-mail: c3ren@mecheng1.uwaterloo.ca [Department of Mechanical and Mechatronics Engineering, University of Waterloo, 200 University Ave West, Waterloo, Ontario, N2L 3G1 (Canada); Pawliszyn, Janusz [Department of Chemistry, University of Waterloo, 200 University Ave West, Waterloo, Ontario, N2L 3G1 (Canada)

    2010-03-10

    A new, simple method was reported to prepare PDMS membranes with micrometer size pores for microfluidic chip applications. The pores were formed by adding polystyrene and toluene into PDMS prepolymer solution prior to spin-coating and curing. The resulting PDMS membrane has a thickness of around 10 {mu}m and macropores with a diameter ranging from 1 to 2 {mu}m measured using scanning electron microscope (SEM) imaging. This PDMS membrane was validated by integrating it with PDMS microfluidic chips for protein separation using isoelectric focusing mechanism coupled with whole channel imaging detection (IEF-WCID). It has been shown that five standard pI markers and a mixture of two proteins, myoglobin and {beta}-lactoglobulin, can be separated using these chips. The results indicated that this macroporous PDMS membrane can replace the dialysis membrane in PDMS chips for the IEF-WCID technique. The preparation method of macroporous PDMS membrane may be potentially applied in other fields of microfluidic chips.

  10. Combination of capillary isoelectric focusing in a tapered capillary with MALDI-TOF MS for rapid and reliable identification of Dickeya species from plant samples

    Czech Academy of Sciences Publication Activity Database

    Horká, Marie; Šalplachta, Jiří; Karásek, Pavel; Kubesová, Anna; Horký, J.; Matoušková, H.; Šlais, Karel; Roth, Michal

    2013-01-01

    Roč. 85, č. 14 (2013), s. 6806-6812 ISSN 0003-2700 R&D Projects: GA MV VG20112015021; GA ČR(CZ) GAP106/12/0522 Institutional support: RVO:68081715 Keywords : capillary isoelectric focusing * matrix-assisted laser desorption/ionization time-of-flight mass spectrometry * Dickeya spp. bacteria Subject RIV: CB - Analytical Chemistry, Separation Impact factor: 5.825, year: 2013

  11. Novel fluorescent probe for highly sensitive bioassay using sequential enzyme-linked immunosorbent assay-capillary isoelectric focusing (ELISA-cIEF).

    Science.gov (United States)

    Henares, Terence G; Uenoyama, Yuta; Nogawa, Yuto; Ikegami, Ken; Citterio, Daniel; Suzuki, Koji; Funano, Shun-ichi; Sueyoshi, Kenji; Endo, Tatsuro; Hisamoto, Hideaki

    2013-06-07

    This paper presents a novel rhodamine diphosphate molecule that allows highly sensitive detection of proteins by employing sequential enzyme-linked immunosorbent assay and capillary isoelectric focusing (ELISA-cIEF). Seven-fold improvement in the immunoassay sensitivity and a 1-2 order of magnitude lower detection limit has been demonstrated by taking advantage of the combination of the enzyme-based signal amplification of ELISA and the concentration of enzyme reaction products by cIEF.

  12. Radioprotective effect against gamma-irradiation of methylene blue in the rat with reference to serum enzymes and pancreatic protein fractions examined by isoelectric focussing

    Energy Technology Data Exchange (ETDEWEB)

    Chung, S O; Nam, S Y [Kyung Hee Univ., Seoul (Korea). Dept. of Biology

    1975-12-01

    The Sprague-Dawley male rats were given 360 rads of single whole-body gamma-irradiation following an intraperitoneal injection of methylene blue (40 mg/kg). Serum glutamic oxaloacetic transaminase (SGOT), serum glutamic pyruvic transaminase (SGPT), serum amylase, serum lipase, and serum lecithinase A activities, and isoelectric focussing pattern of pancreatic juice proteins were determined at various time intervals after exposure. Methylene blue reduced generally the rise of SGOT, sGPT, amylase, and lipase activities. Methylene blue delayed also the serum lecithinase A fall after exposure. Mean number of protein bands as revealed by isoelectric focussing of pancreatic juice were significantly altered in both the control and the methylene blue-treated group after exposure. Especially methylene blue-treated group showed a marked delay in the decrease in number of protein bands after exposure. The possibility of using the SGOT, the SGPT, the serum amylase, the serum lipase, and the serum lecithinase A levels, and the number of protein bands in isoelectric focussing pattern of pancreatic juice as an early index of radiation injury is suggested.

  13. Preparation and characterization of protein isolate from Yellowfin tuna Thunnus albacares roe by isoelectric solubilization/precipitation process

    Directory of Open Access Journals (Sweden)

    Hyun Ji Lee

    2016-05-01

    Full Text Available Abstract Isoelectric solubilization/precipitation (ISP processing allows selective, pH-induced water solubility of proteins with concurrent separation of lipids and removal of materials not intended for human consumption such as bone, scales, skin, etc. Recovered proteins retain functional properties and nutritional value. Four roe protein isolates (RPIs from yellowfin tuna roe were prepared under different solubilization and precipitation condition (pH 11/4.5, pH 11/5.5, pH 12/4.5 and pH 12/5.5. RPIs contained 2.3–5.0 % moisture, 79.1–87.8 % protein, 5.6–7.4 % lipid and 3.0–3.8 % ash. Protein content of RPI-1 and RPI-2 precipitated at pH 4.5 and 5.5 after alkaline solubilization at pH 11, was higher than those of RPI-3 and RPI-4 after alkaline solubilization at pH 12 (P < 0.05. Lipid content (5.6–7.4 % of RPIs was lower than that of freeze-dried concentrate (10.6 %. And leucine and lysine of RPIs were the most abundant amino acids (8.8–9.4 and 8.5–8.9 g/100 g protein, respectively. S, Na, P, K as minerals were the major elements in RPIs. SDS-PAGE of RPIs showed bands at 100, 45, 25 and 15 K. Moisture and protein contents of process water as a 2’nd byproduct were 98.9–99.0 and 1.3–1.8 %, respectively. Therefore, yellowfin tuna roe isolate could be a promising source of valuable nutrients for human food and animal feeds.

  14. Analysis of human bone alkaline phosphatase isoforms: comparison of isoelectric focusing and ion-exchange high-performance liquid chromatography.

    Science.gov (United States)

    Sharp, Christopher A; Linder, Cecilia; Magnusson, Per

    2007-04-01

    Several isoforms of alkaline phosphatase (ALP) can be identified in human tissues and serum after separation by anion-exchange HPLC and isoelectric focusing (IEF). We purified four soluble bone ALP (BALP) isoforms (B/I, B1x, B1 and B2) from human SaOS-2 cells, determined their specific pI values by broad range IEF (pH 3.5-9.5), compared these with commercial preparations of bone, intestinal and liver ALPs and established the effects of neuraminidase and wheat germ lectin (WGA) on enzyme activity. Whilst the isoforms B1x (pI=4.48), B1 (pI=4.32) and B2 (pI=4.12) resolved as well-defined bands, B/I resolved as a complex (pI=4.85-6.84). Neuraminidase altered the migration of all BALP isoforms to pI=6.84 and abolished their binding to the anion-exchange matrix, but increased their enzymatic activities by 11-20%. WGA precipitated the BALP isoforms in IEF gels and the HPLC column and attenuated their enzymatic activities by 54-73%. IEF resolved the commercial BALP into 2 major bands (pI=4.41 and 4.55). Migration of BALP isoforms is similar in IEF and anion-exchange HPLC and dependent on sialic acid content. HPLC is preferable in smaller scale research applications where samples containing mixtures of BALP isoforms are analysed. Circulating liver ALP (pI=3.85) can be resolved from BALP by either method. IEF represents a simpler approach for routine purposes even though some overlapping of the isoforms may occur.

  15. Capillary Isoelectric Focusing-Mass Spectrometry Method for the Separation and Online Characterization of Intact Monoclonal Antibody Charge Variants.

    Science.gov (United States)

    Dai, Jun; Lamp, Jared; Xia, Qiangwei; Zhang, Yingru

    2018-02-06

    We report a new online capillary isoelectric focusing-mass spectrometry (CIEF-MS) method for monoclonal antibody (mAb) charge variant analysis using an electrokinetically pumped sheath-flow nanospray ion source and a time-of-flight MS with pressure-assisted chemical mobilization. To develop a successful, reliable CIEF-MS method for mAb, we have selected and optimized many critical, interrelating reagents and parameters that include (1) MS-friendly anolyte and catholyte; (2) a glycerol enhanced sample mixture that reduced non-CIEF electrophoretic mobility and band broadening; (3) ampholyte selected for balancing resolution and MS sensitivity; (4) sheath liquid composition optimized for efficient focusing, mobilization, and electrospray ionization; (5) judiciously selected CIEF running parameters including injection amount, field strength, and applied pressure. The fundamental premise of CIEF was well maintained as verified by the linear correlation (R 2 = 0.99) between pI values and migration time using a mixture of pI markers. In addition, the charge variant profiles of trastuzumab, bevacizumab, infliximab, and cetuximab, obtained using this CIEF-MS method, were corroborated by imaged CIEF-UV (iCIEF-UV) analyses. The relative standard deviations (RSD) of absolute migration time of pI markers were all less than 5% (n = 4). Triplicate analyses of bevacizumab showed RSD less than 1% for relative migration time to an internal standard and RSD of 7% for absolute MS peak area. Moreover, the antibody charge variants were characterized using the online intact MS data. To the best of our knowledge, this is the first time that direct online MS detection and characterization were achieved for mAb charge variants resolved by CIEF as indicated by a well-established linear pH gradient and correlated CIEF-UV charge variant profiles.

  16. Microfluidic Isoelectric Focusing of Amyloid Beta Peptides Followed by Micropillar-Matrix-Assisted Laser Desorption Ionization-Mass Spectrometry.

    Science.gov (United States)

    Mikkonen, Saara; Jacksén, Johan; Roeraade, Johan; Thormann, Wolfgang; Emmer, Åsa

    2016-10-18

    A novel method for preconcentration and purification of the Alzheimer's disease related amyloid beta (Aβ) peptides by isoelectric focusing (IEF) in 75 nL microchannels combined with their analysis by micropillar-matrix-assisted laser desorption ionization-time-of-flight-mass spectrometry (MALDI-TOF-MS) is presented. A semiopen chip-based setup, consisting of open microchannels covered by a lid of a liquid fluorocarbon, was used. IEF was performed in a mixture of four small and chemically well-defined amphoteric carriers, glutamic acid, aspartyl-histidine (Asp-His), cycloserine (cSer), and arginine, which provided a stepwise pH gradient tailored for focusing of the C-terminal Aβ peptides with a pI of 5.3 in the boundary between cSer and Asp-His. Information about the focusing dynamics and location of the foci of Aβ peptides and other compounds was obtained using computer simulation and by performing MALDI-MS analysis directly from the open microchannel. With the established configuration, detection was performed by direct sampling of a nanoliter volume containing the focused Aβ peptides from the microchannel, followed by deposition of this volume onto a chip with micropillar MALDI targets. In addition to purification, IEF preconcentration provides at least a 10-fold increase of the MALDI-MS-signal. After immunoprecipitation and concentration of the eluate in the microchannel, IEF-micropillar-MALDI-MS is demonstrated to be a suitable platform for detection of Aβ peptides in human cerebrospinal fluid as well as in blood plasma.

  17. Study on a hidden protein-DNA binding in salmon sperm DNA sample by dynamic kinetic capillary isoelectric focusing

    International Nuclear Information System (INIS)

    Liang Liang; Dou Peng; Dong Mingming; Ke Xiaokang; Bian Ningsheng; Liu Zhen

    2009-01-01

    Nuclease P1 is an important enzyme that hydrolyzes RNA or single-stranded DNA into nucleotides, and complete digestion is an essential basis for assays based on this enzyme. To digest a doubled-stranded DNA, the enzyme is usually combined with heat denaturing, which breaks doubled-stranded DNA into single strands. This paper presents an un-expected phenomenon that nuclease P1, in combination with heat denaturing, fails to completely digest a DNA sample extracted from salmon sperm. Under the experimental conditions used, at which nuclease P1 can completely digest calf thymus DNA, the digestion yield of salmon sperm DNA was only 89.5%. Spectrometric measurement indicated that a total protein of 4.7% is present in the DNA sample. To explain the reason for this phenomenon, the dynamic kinetic capillary isoelectric focusing (DK-CIEF) approach proposed previously, which allows for the discrimination of different types of protein-DNA interactions and the measurement of the individual dissociation rate constants, was modified and applied to examine possible protein-DNA interactions involved. It was found that a non-specific DNA-protein binding occurs in the sample, the dissociation rate constant for which was measured to be 7.05 ± 0.83 x 10 -3 s -1 . The formation of DNA-protein complex was suggested to be the main reason for the incomplete digestion of the DNA sample. The modified DK-CIEF approach can be applied as general DNA samples, with the advantages of fast speed and low sample consumption.

  18. Tipping Point

    Medline Plus

    Full Text Available ... en español Blog About OnSafety CPSC Stands for Safety The Tipping Point Home > 60 Seconds of Safety (Videos) > The Tipping Point The Tipping Point by ... danger death electrical fall furniture head injury product safety television tipover tv Watch the video in Adobe ...

  19. Fixed Points

    Indian Academy of Sciences (India)

    Home; Journals; Resonance – Journal of Science Education; Volume 5; Issue 5. Fixed Points - From Russia with Love - A Primer of Fixed Point Theory. A K Vijaykumar. Book Review Volume 5 Issue 5 May 2000 pp 101-102. Fulltext. Click here to view fulltext PDF. Permanent link:

  20. Tipping Point

    Medline Plus

    Full Text Available ... OnSafety CPSC Stands for Safety The Tipping Point Home > 60 Seconds of Safety (Videos) > The Tipping Point ... 24 hours a day. For young children whose home is a playground, it’s the best way to ...

  1. Tipping Point

    Medline Plus

    Full Text Available ... 60 Seconds of Safety (Videos) > The Tipping Point The Tipping Point by CPSC Blogger September 22, 2009 appliance child Childproofing CPSC danger death electrical fall furniture head injury product safety television tipover tv Watch the video in Adobe Flash ...

  2. Electrokinetic properties of PMAA functionalized NiFe2O4 nanoparticles synthesized by thermal plasma route

    Science.gov (United States)

    Bhosale, Shivaji V.; Mhaske, Pravin; Kanhe, N.; Navale, A. B.; Bhoraskar, S. V.; Mathe, V. L.; Bhatt, S. K.

    2014-04-01

    The magnetic nickel ferrite (NiFe2O4) nanoparticles with an average size of 30nm were synthesised by Transferred arc DC Thermal Plasma route. The synthesized nickel ferrite nanoparticles were characterized by TEM and FTIR techniques. The synthesized nickel ferrite nanoparticles were further functionalized with PMAA (polymethacrylic acid) by self emulsion polymerization method and subsequently were characterized by FTIR and Zeta Analyzer. The variation of zeta potential with pH was systematically studied for both PMAA functionalized (PNFO) and uncoated nickel ferrite nanoparticles (NFO). The IEP (isoelectric points) for PNFO and NFO was determined from the graph of zeta potential vs pH. It was observed that the IEP for NFO was at 7.20 and for PNFO it was 2.52. The decrease in IEP of PNFO was attributed to the COOH functional group of PMAA.

  3. Dew Point

    OpenAIRE

    Goldsmith, Shelly

    1999-01-01

    Dew Point was a solo exhibition originating at PriceWaterhouseCoopers Headquarters Gallery, London, UK and toured to the Centre de Documentacio i Museu Textil, Terrassa, Spain and Gallery Aoyama, Tokyo, Japan.

  4. Tipping Point

    Medline Plus

    Full Text Available ... Point by CPSC Blogger September 22, 2009 appliance child Childproofing CPSC danger death electrical fall furniture head injury product safety television tipover tv Watch the video in Adobe Flash ...

  5. Tipping Point

    Science.gov (United States)

    ... Point by CPSC Blogger September 22, 2009 appliance child Childproofing CPSC danger death electrical fall furniture head injury product safety television tipover tv Watch the video in Adobe Flash ...

  6. Tipping Point

    Medline Plus

    Full Text Available ... Point by CPSC Blogger September 22, 2009 appliance child Childproofing CPSC danger death electrical fall furniture head ... see news reports about horrible accidents involving young children and furniture, appliance and tv tip-overs. The ...

  7. Tipping Point

    Medline Plus

    Full Text Available ... Point by CPSC Blogger September 22, 2009 appliance child Childproofing CPSC danger death electrical fall furniture head ... TV falls with about the same force as child falling from the third story of a building. ...

  8. Tipping Point

    Medline Plus

    Full Text Available ... Tipping Point by CPSC Blogger September 22, 2009 appliance child Childproofing CPSC danger death electrical fall furniture ... about horrible accidents involving young children and furniture, appliance and tv tip-overs. The force of a ...

  9. Possibilities of new materials surface sensibility express determination based on ZnSe-CdS system by pH isoelectric state measurements of the surface state

    Science.gov (United States)

    Kirovskaya, I. A.; Mironova, E. V.; Ushakov, O. V.; Nor, P. E.; Yureva, A. V.; Matyash, Yu I.

    2018-01-01

    A method for determining the hydrogen index of the surfaces isoelectric state (pHiso) at various gases pressures -possible components of the surrounding and technological media has been developed. With its use, changes in pH of binary and more complex semiconductors-components of the new system-ZnSe-CdS under the influence of nitrogen dioxide-have been found. The limiting sensitivity of surfaces - minimum PNO2, causing a change in pH has been estimated. The most active components of ZnSe-CdS system, recommended as materials for measuring cells of NO2, have been revealed. The relationship between the changing patterns with the composition of surface (acid-base) and bulk (in particular, theoretical calculated crystal density) properties has been established, allowing to find the most effective materials for sensor technology and for semiconductor analysis.

  10. Surface properties and dye loading behavior of Zn{sub 2}SnO{sub 4} nanoparticles hydrothermally synthesized using different mineralizers

    Energy Technology Data Exchange (ETDEWEB)

    Annamalai, Alagappan; Eo, Yang Dam [Department of Advanced Technology Fusion, Konkuk University, 1 Hwayang-dong, Kwangjin-gu, Seoul 143-701 (Korea, Republic of); Im, Chan [Department of Chemistry, Konkuk University, 1 Hwayang-dong, Kwangjin-gu, Seoul 143-701 (Korea, Republic of); Lee, Man-Jong, E-mail: leemtx@konkuk.ac.kr [Department of Advanced Technology Fusion, Konkuk University, 1 Hwayang-dong, Kwangjin-gu, Seoul 143-701 (Korea, Republic of)

    2011-10-15

    We present for the first time the influence of different mineralizers on the isoelectric point (IEP) of zinc stannate (Zn{sub 2}SnO{sub 4}) nanoparticles hydrothermally prepared using three different mineralizers, viz., Na{sub 2}CO{sub 3}, KOH and tert-butyl amine, and the effect of the IEPs on the dye loading behavior of Zn{sub 2}SnO{sub 4} based photoelectrodes in dye sensitized solar cells (DSSCs). To produce highly crystalline, uniform sized Zn{sub 2}SnO{sub 4} nanoparticles, hydrothermal processing parameters, such as reaction temperature, time, and the mineralizers used have been critically adjusted. The structural and morphological features of the as-synthesized Zn{sub 2}SnO{sub 4} nanoparticles have been observed using both scanning and transmission electron microscopy. For the surface state characterization of shape- and size-controlled Zn{sub 2}SnO{sub 4} nanoparticles, the IEPs of Zn{sub 2}SnO{sub 4} surfaces were determined through zeta potential measurements. The IEPs were found to be 5.7, 7.4 and 8.1 for Zn{sub 2}SnO{sub 4} nanoparticles formed using Na{sub 2}CO{sub 3}, KOH and tert-butyl amine, respectively, suggesting that the surface properties of Zn{sub 2}SnO{sub 4} nanoparticles can be manipulated through the choice of the mineralizers used during the hydrothermal reaction. The amount of N719 dye loading on the surfaces of Zn{sub 2}SnO{sub 4} electrodes having different IEPs was also evaluated. It was revealed that the higher the IEP, the higher the dye loading amount, which means that the IEP mainly affects the dye loading at the dye-metal oxide interface. - Highlights: {yields} The effect of various mineralizers on the isoelectric point of Zn{sub 2}SnO{sub 4} was discussed. {yields} The IEP of Zn{sub 2}SnO{sub 4} can be modified by the choice of mineralizer. {yields} Change in IEP affects the surface properties and the morphology of Zn{sub 2}SnO{sub 4} particles. {yields} Modified surface affects the N719 dye loading behaviour of the Zn{sub 2

  11. Surface properties and dye loading behavior of Zn2SnO4 nanoparticles hydrothermally synthesized using different mineralizers

    International Nuclear Information System (INIS)

    Annamalai, Alagappan; Eo, Yang Dam; Im, Chan; Lee, Man-Jong

    2011-01-01

    We present for the first time the influence of different mineralizers on the isoelectric point (IEP) of zinc stannate (Zn 2 SnO 4 ) nanoparticles hydrothermally prepared using three different mineralizers, viz., Na 2 CO 3 , KOH and tert-butyl amine, and the effect of the IEPs on the dye loading behavior of Zn 2 SnO 4 based photoelectrodes in dye sensitized solar cells (DSSCs). To produce highly crystalline, uniform sized Zn 2 SnO 4 nanoparticles, hydrothermal processing parameters, such as reaction temperature, time, and the mineralizers used have been critically adjusted. The structural and morphological features of the as-synthesized Zn 2 SnO 4 nanoparticles have been observed using both scanning and transmission electron microscopy. For the surface state characterization of shape- and size-controlled Zn 2 SnO 4 nanoparticles, the IEPs of Zn 2 SnO 4 surfaces were determined through zeta potential measurements. The IEPs were found to be 5.7, 7.4 and 8.1 for Zn 2 SnO 4 nanoparticles formed using Na 2 CO 3 , KOH and tert-butyl amine, respectively, suggesting that the surface properties of Zn 2 SnO 4 nanoparticles can be manipulated through the choice of the mineralizers used during the hydrothermal reaction. The amount of N719 dye loading on the surfaces of Zn 2 SnO 4 electrodes having different IEPs was also evaluated. It was revealed that the higher the IEP, the higher the dye loading amount, which means that the IEP mainly affects the dye loading at the dye-metal oxide interface. - Highlights: → The effect of various mineralizers on the isoelectric point of Zn 2 SnO 4 was discussed. → The IEP of Zn 2 SnO 4 can be modified by the choice of mineralizer. → Change in IEP affects the surface properties and the morphology of Zn 2 SnO 4 particles. → Modified surface affects the N719 dye loading behaviour of the Zn 2 SnO 4 based DSSCs.

  12. Focalização isoelétrica na identificação das hemoglobinas The isoelectric focusing for hemoglobin identification

    Directory of Open Access Journals (Sweden)

    Luciane Cristina Bertholo

    2006-06-01

    Full Text Available INTRODUÇÃO: Considerando a significativa freqüência das hemoglobinopatias na população brasileira e a necessidade do estabelecimento de metodologia confiável, rápida, reprodutível e possível de ser aplicada a um grande número de amostras, foi objetivo desse trabalho analisar as diferenças observadas pelas metodologias eletroforéticas em acetato de celulose e em ágar amido em comparação com a focalização isoelétrica (IEF e o estabelecimento de um padrão amostral apresentando as principais posições das hemoglobinas anormais, com enfoque nas observadas na população brasileira. CASUÍSTICA E MÉTODOS: O material de estudo foi constituído por amostras de sangue de pacientes laboratoriais e doadores pertencentes à região central do estado de São Paulo, Brasil, e nelas aplicamos os testes de rotina laboratorial. Para efeito de comparação e validade das provas laboratoriais, correlacionamos os resultados dos testes com a técnica de focalização isoelétrica. RESULTADOS E DISCUSSÃO: Os resultados observados em cada procedimento, somados aos dados de literatura, permitiram estabelecer padrões de migração para cada sistema eletroforético das hemoglobinas observadas na população brasileira. Dessa forma foram estruturados quadros com a possibilidade do uso dos mesmos evidenciando a facilidade e a viabilidade dessa técnica por diferentes laboratórios.BACKGROUND: Considering the significant frequency of hemoglobinopathies in the Brazilian population and the necessity of establishing a reliable methodology, quick, reproductive and possible to be applied in a large number of samples, it was the objective of this work to analyze the differences on electrophoretic procedures with cellulose acetate and acid agar compared with isoelectric focusing (IEF, and to establish a standard of migration, presenting main positions of abnormal hemoglobin, based on Brazilian population hemoglobin. MATERIAL AND METHOD: The studied material

  13. Isoelectric solubilization/precipitation as a means to recover protein isolate from striped bass (Morone saxatilis) and its physicochemical properties in a nutraceutical seafood product.

    Science.gov (United States)

    Tahergorabi, Reza; Beamer, Sarah K; Matak, Kristen E; Jaczynski, Jacek

    2012-06-13

    Excessive dietary intake of Na (i.e., NaCl) contributes to hypertension, which is a major risk factor for cardiovascular disease. Normally, NaOH and HCl are used to dissolve and precipitate, respectively, fish muscle proteins in isoelectric solubilization/precipitation (ISP), therefore contributing to increased Na content in the recovered fish protein isolates (FPI). Substitution of NaOH with KOH may decrease the Na content in FPI and, thus, allow development of reduced-Na seafood products. In this study, FPI was recovered with ISP using NaOH or KOH. In order to develop a nutraceutical seafood product, the FPI was extracted with NaCl or KCl-based salt substitute and subjected to cold- or heat-gelation. In addition, standard nutraceutical additives (ω-3 fatty acids-rich oil and dietary fiber) along with titanium dioxide (TiO2) were added to FPI. Color, texture, dynamic rheology, Na and K content, and lipid oxidation of the FPI gels were compared to commercial Alaska pollock surimi gels. FPI gels had greater (p color properties (L*a*b*), and generally better textural properties when compared to surimi gels. Although the ISP-recovered FPI and surimi developed similar final gel elasticity, the proteins in FPI and surimi had different gelation pattern. A reduction (p fish for subsequent development of nutraceutical seafood products tailored for reduction of diet-driven cardiovascular disease.

  14. Diagnostic utility of isoelectric focusing and high performance liquid chromatography in neonatal cord blood screening for thalassemia and non-sickling hemoglobinopathies.

    Science.gov (United States)

    Uaprasert, Noppacharn; Settapiboon, Rung; Amornsiriwat, Supaporn; Sarnthammakul, Patsita; Thanapat, Tassanee; Rojnuckarin, Ponlapat; Sutcharitchan, Pranee

    2014-01-01

    Thalassemia syndromes are highly prevalent in Southeast Asia. In Thailand, high performance liquid chromatography (HPLC) is the most common technique routinely performed in diagnosis of thalassemia and hemoglobinopathies, while isoelectric focusing (IEF) is rarely employed. We compared the diagnostic utility of IEF and HPLC in neonatal screening for thalassemia and non-sickling hemoglobinopathies. Two-hundred and forty-one cord blood samples were analyzed using IEF and HPLC, β-thalassemia short program. The results were correlated with red cell indices and molecular analyses. Hemoglobin (Hb) Bart's was quantified only on IEF. Of 241 newborns, IEF and HPLC yielded 85.4% and 76.4% sensitivity to identify α-thalassemia syndrome, respectively. HbBart's≥2% yielded 100% sensitivity to identify 2 α-globin gene deletions and/or mutations, while MCV≤95fl and MCH≤30pg yielded 100% sensitivity to identify 2 α-globin gene deletions. DNA analysis revealed HbE mutation in all 61 subjects with HbA2>1% on both IEF and HPLC. IEF is an effective method in neonatal screening for thalassemia and non-sickling hemoglobinopathies. The HbBart's level, MCV and MCH are helpful for identifying α-thalassemia. The presence of HbA2 higher than 1% in cord blood indicates HbE carriers in Southeast Asian newborns. © 2013.

  15. In situ photo-immobilised pH gradient isoelectric focusing and zone electrophoresis integrated two-dimensional microfluidic chip electrophoresis for protein separation

    International Nuclear Information System (INIS)

    Lin, Fengmin; Yu, Shiyong; Gu, Le; Zhu, Xuetao; Wang, Jianshe; Zhu, Han; Lu, Yi; Wang, Yihua; Deng, Yulin; Geng, Lina

    2015-01-01

    A method is introduced for open-column photo-induced site-selective immobilization of pH gradients in a layer of PEG-methacrylate in a multi-dimensional microfluidic chip for use in electrophoresis. It has several attractive features: (a) mixtures of fluorescently labelled proteins carbonic anhydrase, catalase and myoglobin in their native state can be separated by pH-gradient isoelectric focusing (IEF) and zone electrophoresis (CZE) using integrated 2D chip electrophoresis; (b) compared to strip packing or monolithic photo-immobilization, it overcomes the shortcomings of free carrier ampholyte-based 2D chip electrophoresis in an easy way; (c) larger amount of sample can be loaded into the open column-mode electrophoresis (d) immobilized pH gradients can be re-used and the chip can be recycled; (e) a multilayer 3D pH gradient is established by a layer-by-layer assembly technique to further increase the separation capacity. In our perception, this strategy has a large potential in microfluidic chip-based separation schemes because of its simplicity, separation power, re-usability, and separation capacity. (author)

  16. Three Three-Axis IEPE Accelerometers on the Inner Liner of a Tire for Finding the Tire-Road Friction Potential Indicators.

    Science.gov (United States)

    Niskanen, Arto; Tuononen, Ari J

    2015-08-05

    Direct tire-road contact friction estimation is essential for future autonomous cars and active safety systems. Friction estimation methods have been proposed earlier for driving conditions in the presence of a slip angle or slip ratio. However, the estimation of the friction from a freely-rolling tire is still an unsolved topic. Knowing the existing friction potential would be beneficial since vehicle control systems could be adjusted before any remarkable tire force has been produced. Since accelerometers are well-known and robust, and thus a promising sensor type for intelligent tires, this study uses three three-axis IEPE accelerometers on the inner liner of a tire to detect friction potential indicators on two equally smooth surfaces with different friction levels. The equal roughness was chosen for both surfaces in order to study the friction phenomena by neglecting the effect of surface texture on vibrations. The acceleration data before the contact is used to differentiate the two friction levels between the tire and the road. In addition, the contact lengths from the three accelerometers are used to validate the acceleration data. A method to differentiate the friction levels on the basis of the acceleration signal is also introduced.

  17. Three Three-Axis IEPE Accelerometers on the Inner Liner of a Tire for Finding the Tire-Road Friction Potential Indicators

    Directory of Open Access Journals (Sweden)

    Arto Niskanen

    2015-08-01

    Full Text Available Direct tire-road contact friction estimation is essential for future autonomous cars and active safety systems. Friction estimation methods have been proposed earlier for driving conditions in the presence of a slip angle or slip ratio. However, the estimation of the friction from a freely-rolling tire is still an unsolved topic. Knowing the existing friction potential would be beneficial since vehicle control systems could be adjusted before any remarkable tire force has been produced. Since accelerometers are well-known and robust, and thus a promising sensor type for intelligent tires, this study uses three three-axis IEPE accelerometers on the inner liner of a tire to detect friction potential indicators on two equally smooth surfaces with different friction levels. The equal roughness was chosen for both surfaces in order to study the friction phenomena by neglecting the effect of surface texture on vibrations. The acceleration data before the contact is used to differentiate the two friction levels between the tire and the road. In addition, the contact lengths from the three accelerometers are used to validate the acceleration data. A method to differentiate the friction levels on the basis of the acceleration signal is also introduced.

  18. Capillary isoelectric focusing of probiotic bacteria from cow's milk in tapered fused silica capillary with off-line matrix-assisted laser desorption/ionization time-of-flight mass spectrometry identification

    Czech Academy of Sciences Publication Activity Database

    Horká, Marie; Karásek, Pavel; Šalplachta, Jiří; Růžička, F.; Vykydalová, Marie; Kubesová, Anna; Dráb, V.; Roth, Michal; Šlais, Karel

    2013-01-01

    Roč. 788, JUL (2013), s. 193-199 ISSN 0003-2670 R&D Projects: GA ČR(CZ) GAP106/12/0522; GA MV VG20102015023 Institutional support: RVO:68081715 Keywords : capillary isoelectric focusing * matrix-assisted laser desorption/ionization time-of-flight mass spectrometry * lactic acid bacteria Subject RIV: CB - Analytical Chemistry, Separation Impact factor: 4.517, year: 2013

  19. Highly efficient proteome analysis with combination of protein pre-fractionation by preparative microscale solution isoelectric focusing and identification by μRPLC-MS/MS with serially coupled long microcolumn.

    Science.gov (United States)

    Tao, Dingyin; Sun, Liangliang; Zhu, Guijie; Liang, Yu; Liang, Zhen; Zhang, Lihua; Zhang, Yukui

    2011-01-01

    To improve the efficiency of proteome analysis, a strategy with the combination of protein pre-fractionation by preparative microscale solution isoelectric focusing, peptide separation by μRPLC with serially coupled long microcolumn and protein identification by ESI-MS/MS was proposed. By preparative microscale solution isoelectric focusing technique, proteins extracted from whole cell lysates of Escherichia coli were fractionated into five chambers divided by isoelectric membranes, respectively with pH range from 3.0 to 4.6, 4.6 to 5.4, 5.4 to 6.2, 6.2 to 7.0 and 7.0 to 10.0. Compared to the traditional on-gel IFF, the protein recovery could be obviously improved to over 95%. Subsequently, the enriched and fractionated proteins in each chamber were digested, and further separated by a 30-cm long serially coupled RP microcolumn. Through the detection by ESI-MS/MS, about 200 proteins were identified in each fraction, and in total 835 proteins were identified even with one-dimensional μRPLC-MS/MS system. All these results demonstrate that by such a combination strategy, highly efficient proteome analysis could be achieved, not only due to the in-solution protein enrichment and pre-fractionation with improved protein recovery but also owing to the increased separation capacity of serially coupled long μRPLC columns. Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  20. Preparation of intact mitochondria using free-flow isoelectric focusing with post-pH gradient sample injection for morphological, functional and proteomics studies

    International Nuclear Information System (INIS)

    He, Yu-Chen; Kong, Fan-Zhi; Fan, Liu-Yin; Wu, Jane Y.; Liu, Xiao-Ping; Li, Jun; Sun, Yan; Zhang, Qiang; Yang, Ying; Wu, Xue-Jing; Xiao, Hua; Cao, Cheng-Xi

    2017-01-01

    Mitochondria play essential roles in both energy metabolism and cell signaling, which are critical for cell survival. Although significant efforts have been invested in understanding mitochondrial biology, methods for intact mitochondria preparation are technically challenging and remain to be improved. New methods for heterogeneous mitochondria purification will therefore boost our understanding on their physiological and biophysical properties. Herein, we developed a novel recycling free-flow isoelectric focusing (RFFIEF) with post-pH gradient sample injection (post-PGSI) for preparative separation of mitochondria. Crude mitochondria of rabbit liver obtained from differential centrifugation were purified by the developed method according to their pI values as six fractions. Transmission electron microscope images revealed that intact mitochondria existed in two fractions of pH 6.24 and 6.61, degenerative mitochondria were in two fractions of pH 5.46 and 5.72, and inner membrane vesicles (IMVs) appeared in the fractions of pH 4.70 and 5.04. Membrane potential measurement proved a dramatic difference between intact mitochondria and IMVs, which reflected the bioactivity of obtained populations. Particularly, proteomics analyses revealed that more number of proteins were identified in the intact fractions than that of IMVs or crude mitochondria, which demonstrated that RFFIEF could be powerful tool for the preparation of intact organelle as well as their proteomic and in-depth biological analysis. - Highlights: • Mitochondrial subpopulation was successfully separated according to their pIs via the developed RFFIEF method. • The post-PGSI method was introduced for the first time to achieve higher recovery of intact mitochondria. • Quick preparation of mitochondria subpopulation via the developed RFFIEF for both pI determination and downstream research.

  1. Assessment of Intrathecal Free Light Chain Synthesis: Comparison of Different Quantitative Methods with the Detection of Oligoclonal Free Light Chains by Isoelectric Focusing and Affinity-Mediated Immunoblotting.

    Science.gov (United States)

    Zeman, David; Kušnierová, Pavlína; Švagera, Zdeněk; Všianský, František; Byrtusová, Monika; Hradílek, Pavel; Kurková, Barbora; Zapletalová, Olga; Bartoš, Vladimír

    2016-01-01

    We aimed to compare various methods for free light chain (fLC) quantitation in cerebrospinal fluid (CSF) and serum and to determine whether quantitative CSF measurements could reliably predict intrathecal fLC synthesis. In addition, we wished to determine the relationship between free kappa and free lambda light chain concentrations in CSF and serum in various disease groups. We analysed 166 paired CSF and serum samples by at least one of the following methods: turbidimetry (Freelite™, SPAPLUS), nephelometry (N Latex FLC™, BN ProSpec), and two different (commercially available and in-house developed) sandwich ELISAs. The results were compared with oligoclonal fLC detected by affinity-mediated immunoblotting after isoelectric focusing. Although the correlations between quantitative methods were good, both proportional and systematic differences were discerned. However, no major differences were observed in the prediction of positive oligoclonal fLC test. Surprisingly, CSF free kappa/free lambda light chain ratios were lower than those in serum in about 75% of samples with negative oligoclonal fLC test. In about a half of patients with multiple sclerosis and clinically isolated syndrome, profoundly increased free kappa/free lambda light chain ratios were found in the CSF. Our results show that using appropriate method-specific cut-offs, different methods of CSF fLC quantitation can be used for the prediction of intrathecal fLC synthesis. The reason for unexpectedly low free kappa/free lambda light chain ratios in normal CSFs remains to be elucidated. Whereas CSF free kappa light chain concentration is increased in most patients with multiple sclerosis and clinically isolated syndrome, CSF free lambda light chain values show large interindividual variability in these patients and should be investigated further for possible immunopathological and prognostic significance.

  2. Diagnostic applications of newborn screening for α-thalassaemias, haemoglobins E and H disorders using isoelectric focusing on dry blood spots.

    Science.gov (United States)

    Jindatanmanusan, Punyanuch; Riolueang, Suchada; Glomglao, Waraporn; Sukontharangsri, Yaowapa; Chamnanvanakij, Sangkae; Torcharus, Kitti; Viprakasit, Vip

    2014-03-01

    Neonatal screening for haemoglobin (Hb) disorders is a standard of care in several developed countries with the main objective to detect Hb S. Such practice has not been established in Thailand where α-thalassaemia and haemoglobin E (Hb E) are highly prevalent. Early identification of thalassaemias could be helpful and strengthen the programme for prevention and control for severe thalassaemias. Data from isoelectric focusing (IEF) and Isoscan® for detecting types and amount (%) of each haemoglobin in 350 newborn's dried blood spots were analysed and compared with the comprehensive genotype analysis by DNA studies as a gold standard. Based on genetic profiles, there were 10 different categories: (1) normal (n = 227), (2) α(+)-thalassaemia trait (n = 14), (3) α(0)-thalassaemia trait (n = 13), (4) β(0)-thalassaemia trait (n = 7), (5) Hb E trait (n = 72), (6) Hb E trait with α(0)-thalassaemia or homozygous α(+)-thalassaemia (n = 5), (7) Hb E trait with α(+)-thalassaemia trait (n = 5), (8) homozygous Hb E (n = 3), (9) homozygous Hb E with α(0)-thalassaemia trait (n = 1) and (10) Hb H disease (n = 3). The presence of Hb Bart's and Hb E were used to identify cases with α-thalassaemia and Hb E, respectively. We set 0.25% of Hb Bart's and 1.5% of Hb E as a cut-off level to detect α(+)-thalassaemia trait (sensitivity 92.86% and specificity 74.0%) and Hb E trait with 100% of both sensitivity and specificity for IEF diagnosis. Although molecular diagnosis seems to be better for definitive diagnosis of thalassaemia syndromes at birth, however, using our reference range described herein, IEF can be applied in a resource-limiting setting with acceptable reliability.

  3. Study of colloidal particles behaviour in the PWR primary circuit conditions; Etude du comportement des particules colloidales dans les conditions physicochimiques du circuit primaire des reacteurs a eau sous pression

    Energy Technology Data Exchange (ETDEWEB)

    Barale, M

    2006-12-15

    EDF wants to understand, model and limit primary circuit contamination of Pressurized Water Reactors by colloidal particles resulting from corrosion. The electrostatic behaviour of representative oxide particles (cobalt ferrite, nickel ferrite and magnetite) has been studied in primary circuit conditions with the influence of boric acid and lithium hydroxide. The isoelectric point (IEP) and the point of zero charge (PZC) of particles, measured between 5 C and 320 C, exhibit a minimum towards 200 C. The thermodynamic constants of the protonation equilibrium of surface sites were calculated. When boric acid is added, zeta potential and IEP decrease because of borate ions sorption. On the contrary, there is not effect of lithium ions. The modelling of these results under conditions representative of primary circuit shows that these oxides exhibit a negative surface charge, explaining their sorption and adhesion behaviour. (author)

  4. Study of colloidal particles behaviour in the PWR primary circuit conditions

    International Nuclear Information System (INIS)

    Barale, M.

    2006-12-01

    EDF wants to understand, model and limit primary circuit contamination of Pressurized Water Reactors by colloidal particles resulting from corrosion. The electrostatic behaviour of representative oxide particles (cobalt ferrite, nickel ferrite and magnetite) has been studied in primary circuit conditions with the influence of boric acid and lithium hydroxide. The isoelectric point (IEP) and the point of zero charge (PZC) of particles, measured between 5 C and 320 C, exhibit a minimum towards 200 C. The thermodynamic constants of the protonation equilibrium of surface sites were calculated. When boric acid is added, zeta potential and IEP decrease because of borate ions sorption. On the contrary, there is not effect of lithium ions. The modelling of these results under conditions representative of primary circuit shows that these oxides exhibit a negative surface charge, explaining their sorption and adhesion behaviour. (author)

  5. Quantification of trace elements in protein bands by synchrotron radiation x-ray fluorescence after isoelectric focusing separation of human hemoglobin

    International Nuclear Information System (INIS)

    Gao Yuxi; Chen Chunying; Li Bai; He Wei; Huang Yuying; Chai Zhifang

    2005-01-01

    The role and effects of a trace element in a particular organism strongly depend on its particular chemical forms in which the element is present. Therefore, the bulk content or concentration of an element in the organism of interest is often meaningless in judging its biological significance. To understand bioavailability, transportation, cell uptake, metabolism, toxicity, and other biological behaviors of trace elements in the body, information is needed about speciation of trace element, especially about distribution of metal-containing proteins. Development of appropriate methods for speciation analysis is therefore required. Synchrotron radiation x-ray fluorescence (SRXRF) is a sensitive method for multielemental analysis with detection limit of 10 ng/g. It has been successfully used for imaging and quantifying trace elements in various pathological and healthy tissues, even in a single cell, to help understand the mechanism of diseases and the biochemistry of elements. In our previous work, the technique was combined with electrophoresis to study distribution of metalloproteins in biological samples, but the quantitative analysis of trace elements in protein bands after electrophoresis was still unrealized. In this study, a procedure has been proposed for quantification of Fe, Cu, and Zn in protein bands with SRXRF analysis after isoelectric focusing (IEF) separation. Calibration standards were prepared by adding certain amounts of metal ions and free-metal proteins to electrophoresis gel. Human hemoglobin was separated with IEF, and Fe, Cu, and Zn in protein bands were analyzed by SRXRF. The calibration curves can be obtained in a range of 0-8 mg/kg metals and a linear relationship between dosage of metals and fluorescent intensity can be observed (r 2 > 0.99). The method provides the detection limits of 2.43, 1.12, and 0.96 mg/kg for Fe, Cu and Zn, and the recoveries of 90.4 and 115.7 % for Fe and Zn, respectively. The hyphenated technique of SRXRF and IEF

  6. Interactions between whey proteins and kaolinite surfaces

    International Nuclear Information System (INIS)

    Barral, S.; Villa-Garcia, M.A.; Rendueles, M.; Diaz, M.

    2008-01-01

    The nature of the interactions between whey proteins and kaolinite surfaces was investigated by adsorption-desorption experiments at room temperature, performed at the isoelectric point (IEP) of the proteins and at pH 7. It was found that kaolinite is a strong adsorbent for proteins, reaching the maximum adsorption capacity at the IEP of each protein. At pH 7.0, the retention capacity decreased considerably. The adsorption isotherms showed typical Langmuir characteristics. X-ray diffraction data for the protein-kaolinite complexes showed that protein molecules were not intercalated in the mineral structure, but immobilized at the external surfaces and the edges of the kaolinite. Fourier transform IR results indicate the absence of hydrogen bonding between kaolinite surfaces and the polypeptide chain. The adsorption patterns appear to be related to electrostatic interactions, although steric effects should be also considered

  7. Interactions between whey proteins and kaolinite surfaces

    Energy Technology Data Exchange (ETDEWEB)

    Barral, S. [Department of Chemical Engineering and Environmental Technology, University of Oviedo, Julian Claveria 8, 33006 Oviedo (Spain); Villa-Garcia, M.A. [Department of Organic and Inorganic Chemistry, University of Oviedo, Julian Claveria 8, 33006 Oviedo (Spain)], E-mail: mavg@uniovi.es; Rendueles, M. [Project Management Area, University of Oviedo, Independencia 13, 33004 Oviedo (Spain); Diaz, M. [Department of Chemical Engineering and Environmental Technology, University of Oviedo, Julian Claveria 8, 33006 Oviedo (Spain)

    2008-07-15

    The nature of the interactions between whey proteins and kaolinite surfaces was investigated by adsorption-desorption experiments at room temperature, performed at the isoelectric point (IEP) of the proteins and at pH 7. It was found that kaolinite is a strong adsorbent for proteins, reaching the maximum adsorption capacity at the IEP of each protein. At pH 7.0, the retention capacity decreased considerably. The adsorption isotherms showed typical Langmuir characteristics. X-ray diffraction data for the protein-kaolinite complexes showed that protein molecules were not intercalated in the mineral structure, but immobilized at the external surfaces and the edges of the kaolinite. Fourier transform IR results indicate the absence of hydrogen bonding between kaolinite surfaces and the polypeptide chain. The adsorption patterns appear to be related to electrostatic interactions, although steric effects should be also considered.

  8. Incorporation of Tin on copper clad laminate to increase the interface adhesion for signal loss reduction of high-frequency PCB lamination

    Science.gov (United States)

    Wang, Chong; Wen, Na; Zhou, Guoyun; Wang, Shouxu; He, Wei; Su, Xinhong; Hu, Yongsuan

    2017-11-01

    A novel method of improving the adhesion between copper and prepreg in high frequency PCB was proposed and studied in this work. This process which aimed to decrease the IEP (isoelectric point) of the copper to obtain higher adhesion, was achieved by depositing a thin tin layer with lower IEP on copper. It was characterized by scanning electron microscopy (SEM), 3D microscope, peel strength test, X-Ray thickness test, grazing incidence X-ray diffraction (GXRD), X-ray photoelectron spectroscopy (XPS), Agilent vector network analyzer (VNA), which confirmed its excellent adhesion performance and outstanding electrical properties in high-frequency signal transmission compared with traditional brown oxide method. Moreover, the mechanism of achieving high adhesion for this method was also investigated.

  9. Electrokinetic characteristics fused quartz in solutions of 1:1, 2:1 and 3:1 charge electrolytes

    International Nuclear Information System (INIS)

    Bogdanova, N.F.; Sidorova, M.P.; Ermakova, L.Eh.; Savina, I.A.

    1997-01-01

    Electrokinetic characteristics of silicon oxide have been studied using a model system - a plane-parallel capillary in chloride solutions containing mono-(H + , Na + , Cs + ), two-(Ba 2+ ) and three-(La 3+ ) charge counterions in a wide range of pH and concentrations. It has been revealed that isoelectric point (IEP) of silicon oxide studied coincides with the one usually quoted in literature and corresponds to pH2 in the absence of specific adsorption. Specific adsorption of cesium ions resulting in IEP displacement to pH 3.3 at the back-ground of 0.1M CsCl solution has been detected. Specific adsorption of lanthanum ions increases with increase in the surface charge, involving appearance of a positive electrokinetic potential range at pH>3.3 at the background of 0.1g-eq/l LaCl 3 solution

  10. Electrokinetic properties of tantalum oxide deposited on model substrate in NaCl and LiCl solutions

    International Nuclear Information System (INIS)

    Sidorova, M.P.; Bogdanova, N.F.; Ermakova, L.Eh.; Bobrov, P.V.

    1997-01-01

    Electrokinetic characteristics of tantalum oxide have been studied using a model system - a plane-parallel capillary in chloride solutions containing monocharge (H + , Na + , Li + ) counterions in a wide range of pH and concentrations. It is shown that position of isoelectric point (IEP) of Ta 2 O 5 depends on concentration and type of counterion, moreover, the dependence is not explained in the framework of classical notions of the influence of counterion specific adsorption on IEP position. Electrokinetic potential of Ta 2 O-5 surface at the background of diluted LiCl solutions is higher in its absolute value, than at the background of NaCl solutions according to direct lyotropic series. The results of measurements of the capillary resistance dependence on pH at the background of NaCl and LiCl solutions 10 -3 -10 -1 M are used for the calculation of efficiency and specific surface conductivity factors

  11. Development physicochemical and catalytic characteristics of Mo-containing catalysts for hydrotreatment based on various supports. 1. Adsorption of molybdate anions on the support surface

    International Nuclear Information System (INIS)

    Lur'e, M.A.; Kurest, I.Z.; Krasnopol'skaya, S.M.; Reznikov, S.A.; Babikov, A.F.; Shmidt, F.K.

    1994-01-01

    The amounts of basic OH-groups were determined by means of exchange by F-ions and the adsorption of Mo from acid and alkali ammonium paramolybdate (APM) solutions was investigated on the surface of hydrated titanium dioxide, γ-Al 2 O 3 and palygorskite-montmorillonite clay. The process is adequately described by the exchange equation at pH value of APM solution in excess of the isoelectric point (IEP) of the surface. At opposite correlation between pH of the solution and IEP the Langmuir model is adaptable. They concluded, on experimental data, that in the latter case OH-groups replaced by molybdate-anion stage of synthesis of catalyst. 22 refs., 3 figs

  12. Kinetics of adsorption of zinc-ions by doped manganese dioxides

    International Nuclear Information System (INIS)

    Rophael, M.W.; Mourad, W.E.; Khalil, L.B.; Malati, M.A.

    1979-01-01

    Using 65 Zn-labelled solutions, the kinetics of the specific adsorption of Zn 2+ -ions by doped manganese dioxides was studied, at pH values corresponding to their isoelectric points (IEP's), by γ-ray scintillation counting. The rate of the fast adsorption of Zn 2+ -ions by doped dioxides increased in the series: Li + -doped 2+ -ions in about 10 minutes exhibited a similar increase. Compared to the undoped sample, the extent and the rate of adsorption were lower for the samples doped with an ion of valence 4. (author)

  13. Yield stress of alumina-zirconia suspensions

    International Nuclear Information System (INIS)

    Ramakrishnan, V.; Pradip; Malghan, S.G.

    1996-01-01

    The yield stress of concentrated suspensions of alumina, zirconia, and mixed alumina-zirconia powders was measured by the vane technique as a function of solids loading, relative amounts of alumina and zirconia, and pH. At the isoelectric point (IEP), the yield stress varied as the fourth power of the solids loading. The relative ratio of alumina and zirconia particles was important in determining the yield stress of the suspension at the IEP. The yield stress of single and mixed suspensions showed a marked variation with pH. The maximum value occurred at or near the IEP of the suspension. The effect of electrical double-layer forces on the yield stress can be described on the basis of the Derjaguin-Landau-Verwey-Overbeek (DLVO) theory. A normalized yield stress--that is, the ratio of the yield stress at a given pH to the yield stress at the IEP predicted by this model--showed good correlation with experimental data

  14. Programa de coordinación motriz fina en el aprendizaje de la escritura en niños de cinco años de la I.E.P. “Santa María Reina” del distrito de Comas 2016

    OpenAIRE

    Campos Carhuanina, Elizabeth Mercedes

    2017-01-01

    El presente trabajo de investigación, tuvo como problema general: ¿Cuál será el efecto después de aplicar el Programa de Coordinación motriz fina en el aprendizaje de la escritura en niños de cinco años de la I.E.P. Santa María Reina- Comas- 2016? .Y cuyo objetivo general fue Demostrar que efecto produce al aplicar el Programa de Coordinación motriz en el aprendizaje de la escritura en niños de cinco años de la I.E.P. Santa María Reina- Comas- 2016. El tipo de investigaci...

  15. The End of Points

    Science.gov (United States)

    Feldman, Jo

    2018-01-01

    Have teachers become too dependent on points? This article explores educators' dependency on their points systems, and the ways that points can distract teachers from really analyzing students' capabilities and achievements. Feldman argues that using a more subjective grading system can help illuminate crucial information about students and what…

  16. Demerit points systems.

    NARCIS (Netherlands)

    2006-01-01

    In 2012, 21 of the 27 EU Member States had some form of demerit points system. In theory, demerit points systems contribute to road safety through three mechanisms: 1) prevention of unsafe behaviour through the risk of receiving penalty points, 2) selection and suspension of the most frequent

  17. Point specificity in acupuncture

    Directory of Open Access Journals (Sweden)

    Choi Emma M

    2012-02-01

    Full Text Available Abstract The existence of point specificity in acupuncture is controversial, because many acupuncture studies using this principle to select control points have found that sham acupoints have similar effects to those of verum acupoints. Furthermore, the results of pain-related studies based on visual analogue scales have not supported the concept of point specificity. In contrast, hemodynamic, functional magnetic resonance imaging and neurophysiological studies evaluating the responses to stimulation of multiple points on the body surface have shown that point-specific actions are present. This review article focuses on clinical and laboratory studies supporting the existence of point specificity in acupuncture and also addresses studies that do not support this concept. Further research is needed to elucidate the point-specific actions of acupuncture.

  18. Interesting Interest Points

    DEFF Research Database (Denmark)

    Aanæs, Henrik; Dahl, Anders Lindbjerg; Pedersen, Kim Steenstrup

    2012-01-01

    on spatial invariance of interest points under changing acquisition parameters by measuring the spatial recall rate. The scope of this paper is to investigate the performance of a number of existing well-established interest point detection methods. Automatic performance evaluation of interest points is hard......Not all interest points are equally interesting. The most valuable interest points lead to optimal performance of the computer vision method in which they are employed. But a measure of this kind will be dependent on the chosen vision application. We propose a more general performance measure based...... position. The LED illumination provides the option for artificially relighting the scene from a range of light directions. This data set has given us the ability to systematically evaluate the performance of a number of interest point detectors. The highlights of the conclusions are that the fixed scale...

  19. Glucocorticoid effects on hippocampal protein synthesis

    International Nuclear Information System (INIS)

    Schlatter, L.K.

    1988-01-01

    Following subcutaneous injection of rats with 5 mg corticosterone, hippocampal slices in vitro show increased [ 35 S]-methionine labeling of a cytosolic protein with an apparent molecular weight (M r ) of 35,000 and an isoelectric point (IEP) of 6.6. This labeling is temporally consistent with a transcriptional event, and is steroid- and tissue-specific. The pear serum concentration of steroid occurs one hour or less following the injection. Maximal labeling of this protein is reached whenever serum corticosterone values are approximately 100 ng/ml. When endogenous corticosterone levels are elevated to 100 ng/ml through stressors or exogenous ACTH injections the same maximal increase in synthesis of the 35,000 M r protein is observed. Adrenalectomy prevents the observed response from occurring following stressor application or ACTH injections. Comparison of the increases observed after administration of the type 2 receptor agonist RU 28362 and aldosterone, which has a higher affinity for the type 1 receptor, shows a 50-fold greater sensitivity of the response to the type 2 receptor agonist. Synthesis of this protein following serum increases of steroid possibly correlates to the theorized function of the type 2 receptor feedback regulation. The similar protein in the liver has an IEP of 6.8 and a slightly higher M r . A second hippocampal protein with an M r of 46,000 and an IEP of 6.2 is also increased in labeling. Two additional liver proteins, one of Mr 53,000 (IEP of 6.2) and the other with an M r of 45,000 (IEP of 8.7-7.8) are increased in the liver following glucocorticoid administration

  20. Point defects in solids

    International Nuclear Information System (INIS)

    Anon.

    1978-01-01

    The principal properties of point defects are studied: thermodynamics, electronic structure, interactions with etended defects, production by irradiation. Some measuring methods are presented: atomic diffusion, spectroscopic methods, diffuse scattering of neutron and X rays, positron annihilation, molecular dynamics. Then points defects in various materials are investigated: ionic crystals, oxides, semiconductor materials, metals, intermetallic compounds, carbides, nitrides [fr

  1. Indexing Moving Points

    DEFF Research Database (Denmark)

    Agarwal, Pankaj K.; Arge, Lars Allan; Erickson, Jeff

    2003-01-01

    We propose three indexing schemes for storing a set S of N points in the plane, each moving along a linear trajectory, so that any query of the following form can be answered quickly: Given a rectangle R and a real value t, report all K points of S that lie inside R at time t. We first present an...

  2. Generalized zero point anomaly

    International Nuclear Information System (INIS)

    Nogueira, Jose Alexandre; Maia Junior, Adolfo

    1994-01-01

    It is defined Zero point Anomaly (ZPA) as the difference between the Effective Potential (EP) and the Zero point Energy (ZPE). It is shown, for a massive and interacting scalar field that, in very general conditions, the renormalized ZPA vanishes and then the renormalized EP and ZPE coincide. (author). 3 refs

  3. Poisson branching point processes

    International Nuclear Information System (INIS)

    Matsuo, K.; Teich, M.C.; Saleh, B.E.A.

    1984-01-01

    We investigate the statistical properties of a special branching point process. The initial process is assumed to be a homogeneous Poisson point process (HPP). The initiating events at each branching stage are carried forward to the following stage. In addition, each initiating event independently contributes a nonstationary Poisson point process (whose rate is a specified function) located at that point. The additional contributions from all points of a given stage constitute a doubly stochastic Poisson point process (DSPP) whose rate is a filtered version of the initiating point process at that stage. The process studied is a generalization of a Poisson branching process in which random time delays are permitted in the generation of events. Particular attention is given to the limit in which the number of branching stages is infinite while the average number of added events per event of the previous stage is infinitesimal. In the special case when the branching is instantaneous this limit of continuous branching corresponds to the well-known Yule--Furry process with an initial Poisson population. The Poisson branching point process provides a useful description for many problems in various scientific disciplines, such as the behavior of electron multipliers, neutron chain reactions, and cosmic ray showers

  4. The Lagrangian Points

    Science.gov (United States)

    Linton, J. Oliver

    2017-01-01

    There are five unique points in a star/planet system where a satellite can be placed whose orbital period is equal to that of the planet. Simple methods for calculating the positions of these points, or at least justifying their existence, are developed.

  5. Publication point indicators

    DEFF Research Database (Denmark)

    Elleby, Anita; Ingwersen, Peter

    2010-01-01

    ; the Cumulated Publication Point Indicator (CPPI), which graphically illustrates the cumulated gain of obtained vs. ideal points, both seen as vectors; and the normalized Cumulated Publication Point Index (nCPPI) that represents the cumulated gain of publication success as index values, either graphically......The paper presents comparative analyses of two publication point systems, The Norwegian and the in-house system from the interdisciplinary Danish Institute of International Studies (DIIS), used as case in the study for publications published 2006, and compares central citation-based indicators...... with novel publication point indicators (PPIs) that are formalized and exemplified. Two diachronic citation windows are applied: 2006-07 and 2006-08. Web of Science (WoS) as well as Google Scholar (GS) are applied to observe the cite delay and citedness for the different document types published by DIIS...

  6. Multispectral Image Feature Points

    Directory of Open Access Journals (Sweden)

    Cristhian Aguilera

    2012-09-01

    Full Text Available This paper presents a novel feature point descriptor for the multispectral image case: Far-Infrared and Visible Spectrum images. It allows matching interest points on images of the same scene but acquired in different spectral bands. Initially, points of interest are detected on both images through a SIFT-like based scale space representation. Then, these points are characterized using an Edge Oriented Histogram (EOH descriptor. Finally, points of interest from multispectral images are matched by finding nearest couples using the information from the descriptor. The provided experimental results and comparisons with similar methods show both the validity of the proposed approach as well as the improvements it offers with respect to the current state-of-the-art.

  7. Lembranças/memórias de estudante/professora sobre o Instituto de Educação do Pará (IEP em tempos de ditadura, de 1964 a 1985

    Directory of Open Access Journals (Sweden)

    Maria José Aviz do Rosário

    2016-02-01

    Full Text Available Este artigo pretende por meio de lembranças/Memórias de uma estudante/professora, reconstruir passos do IEP, no período, da ditadura militar de 1964 a 1985, com o objetivo de ser mais um elemento de reflexão junto ao projeto de pesquisa, “FORM(AÇÃO DE PROFESSORAS/ES EM TEMPOS DE DITADURA: o Instituto de Educação do Pará no período de 1964 a 1985”. ICED-UFPA-CNPQ e desse modo, contribuir para com a história e memória da educação paraense e brasileira. Os relatos apresentam a forma como era concebida a educação pelos governos do militares no Estado do Pará e como os alunos/professores desse educandário se organizavam para lutar contra a ditadura e por um currículo de ensino menos esvaziado de conhecimento necessário a formação ideológico/política. A organização dos movimentos de resistência criadas no interior da instituição foi fundamental para a formação de professores comprometidos com a justiça social, com a democratização e a qualidade do ensino paraense.

  8. Quantitation of estrogen receptor in seventy-five specimens of breast cancer: comparison between an immunoassay (Abbott ER-EIA monoclonal) and a [3H]estradiol binding assay based on isoelectric focusing in polyacrylamide gel

    International Nuclear Information System (INIS)

    Pousette, A.; Gustafsson, S.A.; Thoernblad, A.M.N.; Nordgren, A.; Saellstroem, J.Li.; Lindgren, A.; Sundelin, P.; Gustafsson, J.A.

    1986-01-01

    Quantitation of estrogen receptor has been performed in cytosol prepared from 75 specimens of breast cancer tissue from patients who had not received hormonal therapy. The study was performed in order to compare an immunoassay (Abbott Laboratories, North Chicago, IL) with our currently used method for estrogen receptor analysis based on isoelectric focusing of [ 3 H]estradiol-receptor complex in polyacrylamide gels. Using linear regression analysis, a regression coefficient (slope) of 1.30 and a correlation coefficient of 0.75 were calculated. The differences in results between the two methods are probably partly explained by the fact that the ligand-based method only measures unoccupied receptor, whereas the immunoassay detects the total amount of receptor, resulting in generally slightly higher concentrations with the latter method. However, in five of 75 specimens the ligand-based method gave a considerably higher concentration of estrogen receptor. This was most probably explained by partial proteolysis resulting in the formation of receptor fragment(s), which was undetectable with the immunoassay but detectable with the ligand-based method. These observations underline the importance of careful handling of specimens during the whole immunoassay procedure

  9. A Chip-Capillary Hybrid Device for Automated Transfer of Sample Pre-Separated by Capillary Isoelectric Focusing to Parallel Capillary Gel Electrophoresis for Two-Dimensional Protein Separation

    Science.gov (United States)

    Lu, Joann J.; Wang, Shili; Li, Guanbin; Wang, Wei; Pu, Qiaosheng; Liu, Shaorong

    2012-01-01

    In this report, we introduce a chip-capillary hybrid device to integrate capillary isoelectric focusing (CIEF) with parallel capillary sodium dodecyl sulfate – polyacrylamide gel electrophoresis (SDS-PAGE) or capillary gel electrophoresis (CGE) toward automating two-dimensional (2D) protein separations. The hybrid device consists of three chips that are butted together. The middle chip can be moved between two positions to re-route the fluidic paths, which enables the performance of CIEF and injection of proteins partially resolved by CIEF to CGE capillaries for parallel CGE separations in a continuous and automated fashion. Capillaries are attached to the other two chips to facilitate CIEF and CGE separations and to extend the effective lengths of CGE columns. Specifically, we illustrate the working principle of the hybrid device, develop protocols for producing and preparing the hybrid device, and demonstrate the feasibility of using this hybrid device for automated injection of CIEF-separated sample to parallel CGE for 2D protein separations. Potentials and problems associated with the hybrid device are also discussed. PMID:22830584

  10. Do acupuncture points exist?

    International Nuclear Information System (INIS)

    Yan Xiaohui; Zhang Xinyi; Liu Chenglin; Dang, Ruishan; Huang Yuying; He Wei; Ding Guanghong

    2009-01-01

    We used synchrotron x-ray fluorescence analysis to probe the distribution of four chemical elements in and around acupuncture points, two located in the forearm and two in the lower leg. Three of the four acupuncture points showed significantly elevated concentrations of elements Ca, Fe, Cu and Zn in relation to levels in the surrounding tissue, with similar elevation ratios for Cu and Fe. The mapped distribution of these elements implies that each acupuncture point seems to be elliptical with the long axis along the meridian. (note)

  11. Do acupuncture points exist?

    Energy Technology Data Exchange (ETDEWEB)

    Yan Xiaohui; Zhang Xinyi [Department of Physics, Surface Physics Laboratory (State Key Laboratory), and Synchrotron Radiation Research Center of Fudan University, Shanghai 200433 (China); Liu Chenglin [Physics Department of Yancheng Teachers' College, Yancheng 224002 (China); Dang, Ruishan [Second Military Medical University, Shanghai 200433 (China); Huang Yuying; He Wei [Beijing Synchrotron Radiation Facility, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing 100039 (China); Ding Guanghong [Shanghai Research Center of Acupuncture and Meridian, Pudong, Shanghai 201203 (China)

    2009-05-07

    We used synchrotron x-ray fluorescence analysis to probe the distribution of four chemical elements in and around acupuncture points, two located in the forearm and two in the lower leg. Three of the four acupuncture points showed significantly elevated concentrations of elements Ca, Fe, Cu and Zn in relation to levels in the surrounding tissue, with similar elevation ratios for Cu and Fe. The mapped distribution of these elements implies that each acupuncture point seems to be elliptical with the long axis along the meridian. (note)

  12. Marine Point Forecasts

    Science.gov (United States)

    will link to the zone forecast and then allow further zooming to the point of interest whereas on the Honolulu, HI Chicago, IL Northern Indiana, IN Lake Charles, LA New Orleans, LA Boston, MA Caribou, ME

  13. Critical-point nuclei

    International Nuclear Information System (INIS)

    Clark, R.M.

    2004-01-01

    It has been suggested that a change of nuclear shape may be described in terms of a phase transition and that specific nuclei may lie close to the critical point of the transition. Analytical descriptions of such critical-point nuclei have been introduced recently and they are described briefly. The results of extensive searches for possible examples of critical-point behavior are presented. Alternative pictures, such as describing bands in the candidate nuclei using simple ΔK = 0 and ΔK = 2 rotational-coupling models, are discussed, and the limitations of the different approaches highlighted. A possible critical-point description of the transition from a vibrational to rotational pairing phase is suggested

  14. National Wetlands Inventory Points

    Data.gov (United States)

    Minnesota Department of Natural Resources — Wetland point features (typically wetlands that are too small to be as area features at the data scale) mapped as part of the National Wetlands Inventory (NWI). The...

  15. Allegheny County Address Points

    Data.gov (United States)

    Allegheny County / City of Pittsburgh / Western PA Regional Data Center — This dataset contains address points which represent physical address locations assigned by the Allegheny County addressing authority. Data is updated by County...

  16. Iowa Geologic Sampling Points

    Data.gov (United States)

    Iowa State University GIS Support and Research Facility — Point locations of geologic samples/files in the IGS repository. Types of samples include well cuttings, outcrop samples, cores, drillers logs, measured sections,...

  17. Characterizing fixed points

    Directory of Open Access Journals (Sweden)

    Sanjo Zlobec

    2017-04-01

    Full Text Available A set of sufficient conditions which guarantee the existence of a point x⋆ such that f(x⋆ = x⋆ is called a "fixed point theorem". Many such theorems are named after well-known mathematicians and economists. Fixed point theorems are among most useful ones in applied mathematics, especially in economics and game theory. Particularly important theorem in these areas is Kakutani's fixed point theorem which ensures existence of fixed point for point-to-set mappings, e.g., [2, 3, 4]. John Nash developed and applied Kakutani's ideas to prove the existence of (what became known as "Nash equilibrium" for finite games with mixed strategies for any number of players. This work earned him a Nobel Prize in Economics that he shared with two mathematicians. Nash's life was dramatized in the movie "Beautiful Mind" in 2001. In this paper, we approach the system f(x = x differently. Instead of studying existence of its solutions our objective is to determine conditions which are both necessary and sufficient that an arbitrary point x⋆ is a fixed point, i.e., that it satisfies f(x⋆ = x⋆. The existence of solutions for continuous function f of the single variable is easy to establish using the Intermediate Value Theorem of Calculus. However, characterizing fixed points x⋆, i.e., providing answers to the question of finding both necessary and sufficient conditions for an arbitrary given x⋆ to satisfy f(x⋆ = x⋆, is not simple even for functions of the single variable. It is possible that constructive answers do not exist. Our objective is to find them. Our work may require some less familiar tools. One of these might be the "quadratic envelope characterization of zero-derivative point" recalled in the next section. The results are taken from the author's current research project "Studying the Essence of Fixed Points". They are believed to be original. The author has received several feedbacks on the preliminary report and on parts of the project

  18. Photoacoustic Point Source

    International Nuclear Information System (INIS)

    Calasso, Irio G.; Craig, Walter; Diebold, Gerald J.

    2001-01-01

    We investigate the photoacoustic effect generated by heat deposition at a point in space in an inviscid fluid. Delta-function and long Gaussian optical pulses are used as sources in the wave equation for the displacement potential to determine the fluid motion. The linear sound-generation mechanism gives bipolar photoacoustic waves, whereas the nonlinear mechanism produces asymmetric tripolar waves. The salient features of the photoacoustic point source are that rapid heat deposition and nonlinear thermal expansion dominate the production of ultrasound

  19. Unconventional Quantum Critical Points

    OpenAIRE

    Xu, Cenke

    2012-01-01

    In this paper we review the theory of unconventional quantum critical points that are beyond the Landau's paradigm. Three types of unconventional quantum critical points will be discussed: (1). The transition between topological order and semiclassical spin ordered phase; (2). The transition between topological order and valence bond solid phase; (3). The direct second order transition between different competing orders. We focus on the field theory and universality class of these unconventio...

  20. SharePoint governance

    OpenAIRE

    Ali, Mudassar

    2013-01-01

    Masteroppgave i informasjons- og kommunikasjonsteknologi IKT590 2013 – Universitetet i Agder, Grimstad SharePoint is a web-based business collaboration platform from Microsoft which is very robust and dynamic in nature. The platform has been in the market for more than a decade and has been adapted by large number of organisations in the world. The platform has become larger in scale, richer in features and is improving consistently with every new version. However, SharePoint ...

  1. Publication point indicators

    DEFF Research Database (Denmark)

    Elleby, Anita; Ingwersen, Peter

    2010-01-01

    The paper presents comparative analyses of two publication point systems, The Norwegian and the in-house system from the interdiscplinary Danish Institute for International Studies (DIIS), used as case in the study for publications published 2006, and compares central citation-based indicators...... with novel publication point indicators (PPIs) that are formalized and exemplified. Two diachronic citation windows are applied: 2006-07 and 2006-08. Web of Science (WoS) as well as Google Scholar (GS) are applied to observe the cite delay and citedness for the different document types published by DIIS...... for all document types. Statistical significant correlations were only found between WoS and GS and the two publication point systems in between, respectively. The study demonstrates how the nCPPI can be applied to institutions as evaluation tools supplementary to JCI in various combinations...

  2. High Concentration Protein Ultrafiltration: a Comparative Fouling Assessment

    Science.gov (United States)

    Lim, Y. P.; Mohammad, A. W.

    2018-05-01

    In this paper, the predominant fouling mechanism via pH manipulation in gelatin ultrafiltration (UF) at constant operating pressure was studied. Two 30 kDa molecular weight cut off (MWCO) UF membranes with different hydrophilic/hydrophobic properties were tested at solution pH near gelatin isoelectric point (IEP), pH below and above gelatin’s IEP. The resistance-in-series model was used to determine quantitatively the contribution of each filtration resistance occurred during gelatin UF. The governing fouling mechanisms were investigated using classical blocking laws. The results demonstrated that concentration polarization remain as dominant fouling resistance in gelatin UF, but exceptional case was observed at pH away from gelatin’s IEP, showing that combined reversible and irreversible fouling resistances contributed around 57% and 37%, respectively to the overall fouling resistances. Under all experimental condition tested, permeate flux decline was accurately predicted by all the models studied. Fouling profile was fitted well with “Standard Blocking”, “Intermediate Blocking” and “Cake Filtration” model for regenerated cellulose acetate (RCA) membrane and “Cake Filtration” model for polyethersulphone (PES) membrane.

  3. Plasma polymer-functionalized silica particles for heavy metals removal.

    Science.gov (United States)

    Akhavan, Behnam; Jarvis, Karyn; Majewski, Peter

    2015-02-25

    Highly negatively charged particles were fabricated via an innovative plasma-assisted approach for the removal of heavy metal ions. Thiophene plasma polymerization was used to deposit sulfur-rich films onto silica particles followed by the introduction of oxidized sulfur functionalities, such as sulfonate and sulfonic acid, via water-plasma treatments. Surface chemistry analyses were conducted by X-ray photoelectron spectroscopy and time-of-flight secondary ion mass spectroscopy. Electrokinetic measurements quantified the zeta potentials and isoelectric points (IEPs) of modified particles and indicated significant decreases of zeta potentials and IEPs upon plasma modification of particles. Plasma polymerized thiophene-coated particles treated with water plasma for 10 min exhibited an IEP of less than 3.5. The effectiveness of developed surfaces in the adsorption of heavy metal ions was demonstrated through copper (Cu) and zinc (Zn) removal experiments. The removal of metal ions was examined through changing initial pH of solution, removal time, and mass of particles. Increasing the water plasma treatment time to 20 min significantly increased the metal removal efficiency (MRE) of modified particles, whereas further increasing the plasma treatment time reduced the MRE due to the influence of an ablation mechanism. The developed particulate surfaces were capable of removing more than 96.7% of both Cu and Zn ions in 1 h. The combination of plasma polymerization and oxidative plasma treatment is an effective method for the fabrication of new adsorbents for the removal of heavy metals.

  4. Zeta potential of Polish copper-bearing shale in the absence and presence of flotation frothers

    Directory of Open Access Journals (Sweden)

    Mengsu Peng

    2014-09-01

    Full Text Available In this paper, zeta potential as a function of pH of copper-bearing shale, which is mined in Poland by KGHM, was investigated. The measurements were conducted in water and aqueous solutions of selected flotation frothers. It was established that for investigated copper-bearing shale, after dispersion in water, the isoelectric point (IEP occurs at pH=3.5. Addition of frothers decreased the IEP on the pH scale and the IEP for butanol was 1.93, for MIBC 2.90 and for eicosaethylene glycol hexadecyl ether (C16E20 2.76. In the case of introducing frothers changed, the zeta potential becomes less negative. An empirical equation, having two adjustable parameters, was used in the paper to approximate the course of the zeta potential-pH curve. The equation showed a very good approximation of the zeta potential of the investigated shale either in water or frother aqueous solutions.

  5. Guided selective deposition of nanoparticles by tuning of the surface potential

    Science.gov (United States)

    Eklöf, J.; Stolaś, A.; Herzberg, M.; Pekkari, A.; Tebikachew, B.; Gschneidtner, T.; Lara-Avila, S.; Hassenkam, T.; Moth-Poulsen, K.

    2017-07-01

    Guided deposition of nanoparticles onto different substrates is of great importance for a variety of applications such as biosensing, targeted cancer therapy, anti-bacterial coatings and single molecular electronics. It is therefore important to gain an understanding of what parameters are involved in the deposition of nanoparticles. In this work we have deposited 60 nm, negatively charged, citrate stabilized gold nanoparticles onto microstructures consisting of six different materials, (vanadium (V), silicon dioxide (SiO2), gold (Au), aluminum (Al), copper (Cu) and nickel (Ni)). The samples have then been investigated by scanning electron microscopy to extract the particle density. The surface potential was calculated from the measured surface charge density maps measured by atomic force microscopy while the samples were submerged in a KCl water solution. These values were compared with literature values of the isoelectric points (IEP) of different oxides formed on the metals in an ambient environment. According to measurements, Al had the highest surface potential followed by Ni and Cu. The same trend was observed for the nanoparticle densities. No particles were found on V, SiO2 and Au. The literature values of the IEP showed a different trend compared to the surface potential measurements concluding that IEP is not a reliable parameter for the prediction of NP deposition. Contribution to the Focus Issue Self-assemblies of Inorganic and Organic Nanomaterials edited by Marie-Paule Pileni.

  6. Charge reversible gold nanoparticles for high efficient absorption and desorption of DNA

    Energy Technology Data Exchange (ETDEWEB)

    Wang Can; Zhuang Jiaqi; Jiang Shan; Li Jun; Yang Wensheng, E-mail: wsyang@jlu.edu.cn [Jilin University, State Key Laboratory of Supramolecular Structure and Materials, College of Chemistry (China)

    2012-10-15

    Mercaptoundecylamine and mercaptoundecanoic acid co-modified Au nanoparticles were prepared by two-step ligand exchange of 6-mercaptohexanoic acid modified gold nanoparticles. Such particles terminated by appropriate ratios of the amine and carboxyl groups (R{sub N/C}) were identified to show reversible charge on their surface, which were switchable by pH of the solution. The isoelectric point (IEP) of the particles is tunable by changing the ratios of the amine and carboxyl groups on the particle surfaces. The particles can absorb DNA effectively at pH lower than the IEP driven by the direct electrostatic interactions between DNA and the particle surface. When pH of the solutions was elevated to be higher than the IEP, the absorbed DNA can be released almost completely due to the electrostatic repulsion between the particle surface and DNA. With appropriate R{sub N/C} ratios of 0.8, the absorption and desorption efficiencies of DNA were 97 and 98%, respectively, corresponding an extraction efficiency of 95 %. Such particles with reversible surface charges allow the high efficient extraction of DNA by simply changing pH instead of by changing salt concentration in the conventional salt bridge method.Graphical Abstract.

  7. Anomalous pH-Dependent Nanofluidic Salinity Gradient Power.

    Science.gov (United States)

    Yeh, Li-Hsien; Chen, Fu; Chiou, Yu-Ting; Su, Yen-Shao

    2017-12-01

    Previous studies on nanofluidic salinity gradient power (NSGP), where energy associated with the salinity gradient can be harvested with ion-selective nanopores, all suggest that nanofluidic devices having higher surface charge density should have higher performance, including osmotic power and conversion efficiency. In this manuscript, this viewpoint is challenged and anomalous counterintuitive pH-dependent NSGP behaviors are reported. For example, with equal pH deviation from its isoelectric point (IEP), the nanopore at pH IEP is shown to have smaller surface charge density but remarkably higher NSGP performance than that at pH > IEP. Moreover, for sufficiently low pH, the NSGP performance decreases with lowering pH (increasing nanopore charge density). As a result, a maximum osmotic power density as high as 5.85 kW m -2 can be generated along with a conversion efficiency of 26.3% achieved for a single alumina nanopore at pH 3.5 under a 1000-fold concentration ratio. Using the rigorous model with considering the surface equilibrium reactions on the pore wall, it is proved that these counterintuitive surface-charge-dependent NSGP behaviors result from the pH-dependent ion concentration polarization effect, which yields the degradation in effective concentration ratio across the nanopore. These findings provide significant insight for the design of next-generation, high-performance NSGP devices. © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  8. Investigation on properties of P((MAA-co-DMAEMA)-g-EG) polyampholyte nanogels

    Energy Technology Data Exchange (ETDEWEB)

    Deng Liandong; Zhai Yinglei; Guo Shutao; Jin Fengmin; Xie Zhaopeng; He Xiaohua; Dong Anjie, E-mail: ajdong@tju.edu.c [Tianjin University, School of Chemical Engineering and Technology (China)

    2009-02-15

    P((MAA-co-DMAEMA)-g-EG) polyampholyte nanogels (PANGs) were prepared by distillation-dispersion copolymerization of poly(ethylene glycol) methyl ether methacrylate (MPEGMA), N,N-dimethylaminoethyl methacrylate (DMAEMA), and methacrylic acid (MAA) using acetonitrile (AN) as dispersion medium. The results of FTIR spectra indicate that the composition of P((MAA-co-DMAEMA)-g-EG) PANGs is consistent with the designed structure. The results of TEM and laser particle size analyzer (LPSA) show that P((MAA-co-DMAEMA)-g-EG) PANGs present spherical morphology and a bimodal size distribution after and before swelling. P((MAA-co-DMAEMA)-g-EG) PANGs have typically amphoteric characters responding to pH, whose isoelectric point (IEP) increases with decreasing the ratio of MAA/DMAEMA and equilibrium swelling degree (ESD) is greater than that at IEP when the pH value is distant from IEP. P((MAA-co-DMAEMA)-g-EG) PANGs also represent ionic strength sensitivity. Using the water-soluble chitosan (CS, Mn = 5 kDa) as model drug, in vitro release indicates that CS can be effectively incorporated into PANGs and the release rate of CS at pH 1.89 is an order of magnitude greater than that at pH 8.36. P((MAA-co-DMAEMA)-g-EG) PANGs may be useful in biomedicine, especially in oral drug delivery of biomacromolecule.

  9. Adsorption of citrate ions on hydroxyapatite synthesized by various methods

    International Nuclear Information System (INIS)

    Skwarek, E.; Janusz, W.; Sternik, D.

    2014-01-01

    The specific adsorption of citric acid ions at hydroxyapatite interface was investigated by the means of radioisotope method ( 14 C) as a function of citric acid ions concentration, NaCl concentration and pH. Application of the hydroxyapatite has become wide in the biomaterial field as the Ca 10 (OH) 2 (PO 4 ) 6 possess biocompatibility with human hard tissue. Hydroxyapatite was synthesized using three different methods. The physical properties of the resulting powder were characterized by DTA/TG, XRD, AFM and SEM microscopy. Physicochemical qualities characterizing the electrical double layer of the hydroxyapatite/NaCl solution interface were determined. The zeta potential and the adsorption of citric acid molecule were studied as a function of pH. The point of zero charge and the isoelectric point of samples were determined. Electrical double layer parameters of hydroxyapatite/NaCl interface are influenced by a synthesis method. The points pH pzc and pH IEP for sample 1 are pH pzc 7.5 and pH IEP 3; for sample 2 pH pzc 7.05 and pH IEP 3, for sample 3 pH pzc 6.7 and pH IEP 3. Temperature has weak influence both on pure substance and with citric acid adsorbed, as derivatographic analysis has shown, and characterization of hydroxyapatite structure may be carried out by this thermal analysis. Two phenomena are responsible for citric acid adsorption: phosphate group's replacement at hydroxyapatite surface by citric ions parallel to intraspherical complexes formation. (author)

  10. New Novae snack point

    CERN Document Server

    2012-01-01

    Located next to the car park by the flag poles, a few metres from the Main CERN Reception (building 33), a new snack point catered by Novae will open to the public on Wednesday 8 August. More information will be available in the next issue of the Bulletin!

  11. [Clinical key points. Gonioscopy].

    Science.gov (United States)

    Hamard, P

    2007-05-01

    Gonioscopy should be performed in all patients with glaucoma or suspected of having glaucoma. Four points are systematically evaluated: the width of the angle, the degree of trabecular pigmentation, the iridotrabecular appositions or synechia, and the level of iris insertion and the shape of the peripheral iris profile.

  12. ACS Zero Point Verification

    Science.gov (United States)

    Dolphin, Andrew

    2005-07-01

    The uncertainties in the photometric zero points create a fundamental limit to the accuracy of photometry. The current state of the ACS calibration is surprisingly poor, with zero point uncertainties of 0.03 magnitudes. The reason for this is that the ACS calibrations are based primarily on semi-emprical synthetic zero points and observations of fields too crowded for accurate ground-based photometry. I propose to remedy this problem by obtaining ACS images of the omega Cen standard field with all nine broadband ACS/WFC filters. This will permit the direct determination of the ACS zero points by comparison with excellent ground-based photometry, and should reduce their uncertainties to less than 0.01 magnitudes. A second benefit is that it will facilitate the comparison of the WFPC2 and ACS photometric systems, which will be important as WFPC2 is phased out and ACS becomes HST's primary imager. Finally, three of the filters will be repeated from my Cycle 12 observations, allowing for a measurement of any change in sensitivity.

  13. Point Lepreau generating station

    International Nuclear Information System (INIS)

    Ganong, G.H.D.; Strang, A.E.; Gunter, G.E.; Thompson, T.S.

    Point Lepreau-1 reactor is a 600 MWe generating station expected to be in service by October 1979. New Brunswick is suffering a 'catch up' phenomenon in load growth and needs to decrease dependence on foreign oil. The site is on salt water and extensive study has gone into corrosion control. Project management, financing and scheduling have unique aspects. (E.C.B.)

  14. Least fixed points revisited

    NARCIS (Netherlands)

    J.W. de Bakker (Jaco)

    1975-01-01

    textabstractParameter mechanisms for recursive procedures are investigated. Contrary to the view of Manna et al., it is argued that both call-by-value and call-by-name mechanisms yield the least fixed points of the functionals determined by the bodies of the procedures concerned. These functionals

  15. Point kinetics modeling

    International Nuclear Information System (INIS)

    Kimpland, R.H.

    1996-01-01

    A normalized form of the point kinetics equations, a prompt jump approximation, and the Nordheim-Fuchs model are used to model nuclear systems. Reactivity feedback mechanisms considered include volumetric expansion, thermal neutron temperature effect, Doppler effect and void formation. A sample problem of an excursion occurring in a plutonium solution accidentally formed in a glovebox is presented

  16. Melting point of yttria

    International Nuclear Information System (INIS)

    Skaggs, S.R.

    1977-06-01

    Fourteen samples of 99.999 percent Y 2 O 3 were melted near the focus of a 250-W CO 2 laser. The average value of the observed melting point along the solid-liquid interface was 2462 +- 19 0 C. Several of these same samples were then melted in ultrahigh-purity oxygen, nitrogen, helium, or argon and in water vapor. No change in the observed temperature was detected, with the exception of a 20 0 C increase in temperature from air to helium gas. Post test examination of the sample characteristics, clarity, sphericity, and density is presented, along with composition. It is suggested that yttria is superior to alumina as a secondary melting-point standard

  17. 'Saddle-point' ionization

    International Nuclear Information System (INIS)

    Gay, T.J.; Hale, E.B.; Irby, V.D.; Olson, R.E.; Missouri Univ., Rolla; Berry, H.G.

    1988-01-01

    We have studied the ionization of rare gases by protons at intermediate energies, i.e., energies at which the velocities of the proton and the target-gas valence electrons are comparable. A significant channel for electron production in the forward direction is shown to be 'saddle-point' ionization, in which electrons are stranded on or near the saddle-point of electric potential between the receding projectile and the ionized target. Such electrons yield characteristic energy spectra, and contribute significantly to forward-electron-production cross sections. Classical trajectory Monte Carlo calculations are found to provide qualitative agreement with our measurements and the earlier measurements of Rudd and coworkers, and reproduce, in detail, the features of the general ionization spectra. (orig.)

  18. High boiling point hydrocarbons

    Energy Technology Data Exchange (ETDEWEB)

    Pier, M

    1929-04-29

    A process is given for the production of hydrocarbons of high boiling point, such as lubricating oils, from bituminous substances, such as varieties of coal, shale, or other solid distillable carbonaceous materials. The process consists of treating the initial materials with organic solvents and then subjecting the products extracted from the initial materials, preferably directly, to a reducing treatment in respect to temperature, pressure, and time. The reduction treatment is performed by means of hydrogen under pressure.

  19. Critical point predication device

    International Nuclear Information System (INIS)

    Matsumura, Kazuhiko; Kariyama, Koji.

    1996-01-01

    An operation for predicting a critical point by using a existent reverse multiplication method has been complicated, and an effective multiplication factor could not be plotted directly to degrade the accuracy for the prediction. The present invention comprises a detector counting memory section for memorizing the counting sent from a power detector which monitors the reactor power, a reverse multiplication factor calculation section for calculating the reverse multiplication factor based on initial countings and current countings of the power detector, and a critical point prediction section for predicting the criticality by the reverse multiplication method relative to effective multiplication factors corresponding to the state of the reactor core previously determined depending on the cases. In addition, a reactor core characteristic calculation section is added for analyzing an effective multiplication factor depending on the state of the reactor core. Then, if the margin up to the criticality is reduced to lower than a predetermined value during critical operation, an alarm is generated to stop the critical operation when generation of a period of more than a predetermined value predicted by succeeding critical operation. With such procedures, forecasting for the critical point can be easily predicted upon critical operation to greatly mitigate an operator's burden and improve handling for the operation. (N.H.)

  20. At the Tipping Point

    Energy Technology Data Exchange (ETDEWEB)

    Wiley, H. S.

    2011-02-28

    There comes a time in every field of science when things suddenly change. While it might not be immediately apparent that things are different, a tipping point has occurred. Biology is now at such a point. The reason is the introduction of high-throughput genomics-based technologies. I am not talking about the consequences of the sequencing of the human genome (and every other genome within reach). The change is due to new technologies that generate an enormous amount of data about the molecular composition of cells. These include proteomics, transcriptional profiling by sequencing, and the ability to globally measure microRNAs and post-translational modifications of proteins. These mountains of digital data can be mapped to a common frame of reference: the organism’s genome. With the new high-throughput technologies, we can generate tens of thousands of data points from each sample. Data are now measured in terabytes and the time necessary to analyze data can now require years. Obviously, we can’t wait to interpret the data fully before the next experiment. In fact, we might never be able to even look at all of it, much less understand it. This volume of data requires sophisticated computational and statistical methods for its analysis and is forcing biologists to approach data interpretation as a collaborative venture.

  1. Maximum power point tracking

    International Nuclear Information System (INIS)

    Enslin, J.H.R.

    1990-01-01

    A well engineered renewable remote energy system, utilizing the principal of Maximum Power Point Tracking can be m ore cost effective, has a higher reliability and can improve the quality of life in remote areas. This paper reports that a high-efficient power electronic converter, for converting the output voltage of a solar panel, or wind generator, to the required DC battery bus voltage has been realized. The converter is controlled to track the maximum power point of the input source under varying input and output parameters. Maximum power point tracking for relative small systems is achieved by maximization of the output current in a battery charging regulator, using an optimized hill-climbing, inexpensive microprocessor based algorithm. Through practical field measurements it is shown that a minimum input source saving of 15% on 3-5 kWh/day systems can easily be achieved. A total cost saving of at least 10-15% on the capital cost of these systems are achievable for relative small rating Remote Area Power Supply systems. The advantages at larger temperature variations and larger power rated systems are much higher. Other advantages include optimal sizing and system monitor and control

  2. Vernal Point and Anthropocene

    Science.gov (United States)

    Chavez-Campos, Teodosio; Chavez S, Nadia; Chavez-Sumarriva, Israel

    2014-05-01

    The time scale was based on the internationally recognized formal chronostratigraphical /geochronological subdivisions of time: The Phanerozoic Eonathem/Eon; the Cenozoic Erathem/Era; the Quaternary System/Period; the Pleistocene and Holocene Series/Epoch. The Quaternary was divided into: (1) The Pleistocene that was characterized by cycles of glaciations (intervals between 40,000 and 100,000 years). (2) The Holocene that was an interglacial period that began about 12,000 years ago. It was believed that the Milankovitch cycles (eccentricity, axial tilt and the precession of the equinoxes) were responsible for the glacial and interglacial Holocene periods. The magnetostratigraphic units have been widely used for global correlations valid for Quaternary. The gravitational influence of the sun and moon on the equatorial bulges of the mantle of the rotating earth causes the precession of the earth. The retrograde motion of the vernal point through the zodiacal band is 26,000 years. The Vernal point passes through each constellation in an average of 2000 years and this period of time was correlated to Bond events that were North Atlantic climate fluctuations occurring every ≡1,470 ± 500 years throughout the Holocene. The vernal point retrogrades one precessional degree approximately in 72 years (Gleissberg-cycle) and approximately enters into the Aquarius constellation on March 20, 1940. On earth this entry was verify through: a) stability of the magnetic equator in the south central zone of Peru and in the north zone of Bolivia, b) the greater intensity of equatorial electrojet (EEJ) in Peru and Bolivia since 1940. With the completion of the Holocene and the beginning of the Anthropocene (widely popularized by Paul Crutzen) it was proposed the date of March 20, 1940 as the beginning of the Anthropocene. The date proposed was correlated to the work presented in IUGG (Italy 2007) with the title "Cusco base meridian for the study of geophysical data"; Cusco was

  3. Point/Counterpoint

    DEFF Research Database (Denmark)

    Ungar, David; Ernst, Erik

    2007-01-01

    Point Argument: "Dynamic Languages (in Reactive Environments) Unleash Creativity," by David Ungar. For the sake of creativity, the profession needs to concentrate more on inventing new and better dynamic languages and environments and less on improving static languages. Counterpoint Argument......: "Explicitly Declared Static Types: The Missing Links," by Erik Ernst. How do we understand software? Using it is a powerful approach, but it provides examples of properties, not general truths. Some static knowledge is needed. This department is part of a special issue on dynamically typed languages....

  4. Mise au point

    African Journals Online (AJOL)

    tomie est replacé et fixé par des fils d'acier, krönlein lais- sait ce fragment pédiculé au fascia temporalis afin d'évi- ter la dépression de la fosse temporale due à la désinser- tion du muscle temporal [20] ; dans notre série, après reconstitution du cadre, le muscle temporal est suturé à son point d'insertion. pour les tumeurs ...

  5. Pointing control for LDR

    Science.gov (United States)

    Yam, Y.; Briggs, C.

    1988-01-01

    One important aspect of the LDR control problem is the possible excitations of structural modes due to random disturbances, mirror chopping, and slewing maneuvers. An analysis was performed to yield a first order estimate of the effects of such dynamic excitations. The analysis involved a study of slewing jitters, chopping jitters, disturbance responses, and pointing errors, making use of a simplified planar LDR model which describes the LDR dynamics on a plane perpendicular to the primary reflector. Briefly, the results indicate that the command slewing profile plays an important role in minimizing the resultant jitter, even to a level acceptable without any control action. An optimal profile should therefore be studied.

  6. Point of Care Ultrasound

    DEFF Research Database (Denmark)

    Dietrich, Christoph F; Goudie, Adrian; Chiorean, Liliana

    2017-01-01

    Over the last decade, the use of portable ultrasound scanners has enhanced the concept of point of care ultrasound (PoC-US), namely, "ultrasound performed at the bedside and interpreted directly by the treating clinician." PoC-US is not a replacement for comprehensive ultrasound, but rather allows...... and critical care medicine, cardiology, anesthesiology, rheumatology, obstetrics, neonatology, gynecology, gastroenterology and many other applications. In the future, PoC-US will be more diverse than ever and be included in medical student training....

  7. The point on.

    International Nuclear Information System (INIS)

    1996-01-01

    In this last article, we find the point of view about the world petroleum activity, the reserves and the recent discoveries, the deep offshore, the technological developments in petroleum upstream. The petroleum situation in China is treated. The trends of world refining are described. The recent technological developments in the petroleum downstream are detailed. The prices of crude oil and the refining margins are the subject of a chapter. The investments of hydrocarbons area are given, the world trade and the lng projects, the gas availability in Western Europe have their place. The trends of European automobile industry and the fuels distribution are also discussed. (N.C.)

  8. Relative Critical Points

    Directory of Open Access Journals (Sweden)

    Debra Lewis

    2013-05-01

    Full Text Available Relative equilibria of Lagrangian and Hamiltonian systems with symmetry are critical points of appropriate scalar functions parametrized by the Lie algebra (or its dual of the symmetry group. Setting aside the structures – symplectic, Poisson, or variational – generating dynamical systems from such functions highlights the common features of their construction and analysis, and supports the construction of analogous functions in non-Hamiltonian settings. If the symmetry group is nonabelian, the functions are invariant only with respect to the isotropy subgroup of the given parameter value. Replacing the parametrized family of functions with a single function on the product manifold and extending the action using the (coadjoint action on the algebra or its dual yields a fully invariant function. An invariant map can be used to reverse the usual perspective: rather than selecting a parametrized family of functions and finding their critical points, conditions under which functions will be critical on specific orbits, typically distinguished by isotropy class, can be derived. This strategy is illustrated using several well-known mechanical systems – the Lagrange top, the double spherical pendulum, the free rigid body, and the Riemann ellipsoids – and generalizations of these systems.

  9. Reassessing Function Points

    Directory of Open Access Journals (Sweden)

    G.R. Finnie

    1997-05-01

    Full Text Available Accurate estimation of the size and development effort for software projects requires estimation models which can be used early enough in the development life cycle to be of practical value. Function Point Analysis (FPA has become possibly the most widely used estimation technique in practice. However the technique was developed in the data processing environment of the 1970's and, despite undergoing considerable reassessment and formalisation, still attracts criticism for the weighting scoring it employs and for the way in which the function point score is adapted for specific system characteristics. This paper reviews the validity of the weighting scheme and the value of adjusting for system characteristics by studying their effect in a sample of 299 software developments. In general the value adjustment scheme does not appear to cater for differences in productivity. The weighting scheme used to adjust system components in terms of being simple, average or complex also appears suspect and should be redesigned to provide a more realistic estimate of system functionality.

  10. Bright point study

    International Nuclear Information System (INIS)

    Tang, F.; Harvey, K.; Bruner, M.; Kent, B.; Antonucci, E.

    1982-01-01

    Transition region and coronal observations of bright points by instruments aboard the Solar Maximum Mission and high resolution photospheric magnetograph observations on September 11, 1980 are presented. A total of 31 bipolar ephemeral regions were found in the photosphere from birth in 9.3 hours of combined magnetograph observations from three observatories. Two of the three ephemeral regions present in the field of view of the Ultraviolet Spectrometer-Polarimeter were observed in the C IV 1548 line. The unobserved ephemeral region was determined to be the shortest-lived (2.5 hr) and lowest in magnetic flux density (13G) of the three regions. The Flat Crystal Spectrometer observed only low level signals in the O VIII 18.969 A line, which were not statistically significant to be positively identified with any of the 16 ephemeral regions detected in the photosphere. In addition, the data indicate that at any given time there lacked a one-to-one correspondence between observable bright points and photospheric ephemeral regions, while more ephemeral regions were observed than their counterparts in the transition region and the corona

  11. Referential Zero Point

    Directory of Open Access Journals (Sweden)

    Matjaž Potrč

    2016-04-01

    Full Text Available Perhaps the most important controversy in which ordinary language philosophy was involved is that of definite descriptions, presenting referential act as a community-involving communication-intention endeavor, thereby opposing the direct acquaintance-based and logical proper names inspired reference aimed at securing truth conditions of referential expression. The problem of reference is that of obtaining access to the matters in the world. This access may be forthcoming through the senses, or through descriptions. A review of how the problem of reference is handled shows though that one main practice is to indulge in relations of acquaintance supporting logical proper names, demonstratives, indexicals and causal or historical chains. This testifies that the problem of reference involves the zero point, and with it phenomenology of intentionality. Communication-intention is but one dimension of rich phenomenology that constitutes an agent’s experiential space, his experiential world. Zero point is another constitutive aspect of phenomenology involved in the referential relation. Realizing that the problem of reference is phenomenology based opens a new perspective upon the contribution of analytical philosophy in this area, reconciling it with continental approach, and demonstrating variations of the impossibility related to the real. Chromatic illumination from the cognitive background empowers the referential act, in the best tradition of ordinary language philosophy.

  12. Precipitation and ultimate pH effect on chemical and gelation properties of protein prepared by isoelectric solubilization/precipitation process from pale, soft, exudative (PSE)-like chicken breast meat1.

    Science.gov (United States)

    Zhao, X; Xing, T; Chen, X; Han, M-Y; Li, X; Xu, X-L; Zhou, G-H

    2017-05-01

    Pale, soft, exudative (PSE)-like chicken breast is considered deteriorated raw material in the poultry meat industry that has inferior processing ability. The chemical and gelation properties of PSE-like chicken breast meat paste were studied. These pastes were prepared by the pH adjustment method and protein isolation using the isoelectric solubilization/precipitation (ISP) process from PSE-like chicken meat. The ISP-isolated samples were solubilized at pH 11.0 and recovered at pH 5.5 and 6.2. The ultimate pH of the ISP-isolated protein and meat paste was adjusted to 6.2 and 7.0. The ultimate pH in this article referred to the final pH of the extracted protein and meat paste. Higher reactive sulfhydryl content and surface hydrophobicity were found in the precipitation at pH 6.2 than at pH 5.5. However, various ultimate pH values showed no significant influence on the surface hydrophobicity. The hardness of gel, as measured by textural profile analysis, was improved using 6.2 as the precipitation pH compared with pH 5.5. The viscoelastic modulus (G΄) of gel pastes prior to the thermal gelation was higher with ISP treatment. However, lower G΄ was seen after thermal gelation compared with the control. Dynamic rheological measurement demonstrated a different gel-forming mechanism for protein precipitated at pH values of 5.5 and 6.2 compared with the meat paste. The cooking loss showed that the recovered protein failed to form a gel with good water-retention capacity unless the ultimate pH was adjusted to 7.0. Gels made from protein extracted by the ISP method had higher yellowness and lower redness values, probably due to protein denaturation. Precipitation at pH 6.2 formed a harder gel with lower water-retention ability than that at pH 5.5, and this result was possibly due to higher surface hydrophobicity and S-S bridge formation. Overall, network characteristics of ISP-treated protein gels were strongly dependent on precipitation pH and ultimate pH. © 2016

  13. Point Pollution Sources Dimensioning

    Directory of Open Access Journals (Sweden)

    Georgeta CUCULEANU

    2011-06-01

    Full Text Available In this paper a method for determining the main physical characteristics of the point pollution sources is presented. It can be used to find the main physical characteristics of them. The main physical characteristics of these sources are top inside source diameter and physical height. The top inside source diameter is calculated from gas flow-rate. For reckoning the physical height of the source one takes into account the relation given by the proportionality factor, defined as ratio between the plume rise and physical height of the source. The plume rise depends on the gas exit velocity and gas temperature. That relation is necessary for diminishing the environmental pollution when the production capacity of the plant varies, in comparison with the nominal one.

  14. Critical Points of Contact

    DEFF Research Database (Denmark)

    Jensen, Ole B.; Wind, Simon; Lanng, Ditte Bendix

    2012-01-01

    In this brief article, we shall illustrate the application of the analytical and interventionist concept of ‘Critical Points of Contact’ (CPC) through a number of urban design studios. The notion of CPC has been developed over a span of the last three to four years and is reported in more detail...... elsewhere (Jensen & Morelli 2011). In this article, we will only discuss the conceptual and theoretical framing superficially, since our real interest is to show and discuss the concept's application value to spatial design in a number of urban design studios. The 'data' or the projects presented are seven...... in urban design at Aalborg University, where urban design consists of both an analytical and an interventionist field of operation. Furthermore, the content of the CPC concept links to research in mobilities, the network city, and urban design. These are among the core pillars of both the masters programme...

  15. Point defects in platinum

    International Nuclear Information System (INIS)

    Piercy, G.R.

    1960-01-01

    An investigation was made of the mobility and types of point defect introduced in platinum by deformation in liquid nitrogen, quenching into water from 1600 o C, or reactor irradiation at 50 o C. In all cases the activation energy for motion of the defect was determined from measurements of electrical resistivity. Measurements of density, hardness, and x-ray line broadening were also made there applicable. These experiments indicated that the principal defects remaining in platinum after irradiation were single vacant lattice sites and after quenching were pairs of vacant lattice sites. Those present after deformation In liquid nitrogen were single vacant lattice sites and another type of defect, perhaps interstitial atoms. (author)

  16. Virtual turning points

    CERN Document Server

    Honda, Naofumi; Takei, Yoshitsugu

    2015-01-01

    The discovery of a virtual turning point truly is a breakthrough in WKB analysis of higher order differential equations. This monograph expounds the core part of its theory together with its application to the analysis of higher order Painlevé equations of the Noumi–Yamada type and to the analysis of non-adiabatic transition probability problems in three levels. As M.V. Fedoryuk once lamented, global asymptotic analysis of higher order differential equations had been thought to be impossible to construct. In 1982, however, H.L. Berk, W.M. Nevins, and K.V. Roberts published a remarkable paper in the Journal of Mathematical Physics indicating that the traditional Stokes geometry cannot globally describe the Stokes phenomena of solutions of higher order equations; a new Stokes curve is necessary.

  17. The Temporal Tipping Point

    DEFF Research Database (Denmark)

    Hermann, A. K.

    2016-01-01

    “Slow journalism” is a term anthropologist and sociologists sometimes use to describe their empirical work, ethnography. To journalists and media observers, meanwhile, “slow journalism” signifies a newfound dedication to serious long-form journalism. Not surprisingly, thus, “ethnographic journalism......”—a genre where reporters adopt research strategies from social science—takes “slow” to the extreme. Immersing themselves in communities for weeks, months and years, ethnographic journalists seek to gain what anthropologists call “the native's point of view”. Based on in-depth interviews with practitioners...... and analyses of their journalistic works, this paper offers a study of ethnographic journalism suggesting that slow time operates in at least three separate registers. First, in terms of regimentation, ethnographic journalism is mostly long-form pieces that demand time-consuming research and careful writing...

  18. The Point Mass Concept

    Directory of Open Access Journals (Sweden)

    Lehnert B.

    2011-04-01

    Full Text Available A point-mass concept has been elaborated from the equations of the gravitational field. One application of these deductions results in a black hole configuration of the Schwarzschild type, having no electric charge and no angular momentum. The critical mass of a gravitational collapse with respect to the nuclear binding energy is found to be in the range of 0.4 to 90 solar masses. A second application is connected with the spec- ulation about an extended symmetric law of gravitation, based on the options of positive and negative mass for a particle at given positive energy. This would make masses of equal polarity attract each other, while masses of opposite polarity repel each other. Matter and antimatter are further proposed to be associated with the states of positive and negative mass. Under fully symmetric conditions this could provide a mechanism for the separation of antimatter from matter at an early stage of the universe.

  19. The Point Mass Concept

    Directory of Open Access Journals (Sweden)

    Lehnert B.

    2011-04-01

    Full Text Available A point-mass concept has been elaborated from the equations of the gravitational field. One application of these deductions results in a black hole configuration of the Schwarzschild type, having no electric charge and no angular momentum. The critical mass of a gravitational collapse with respect to the nuclear binding energy is found to be in the range of 0.4 to 90 solar masses. A second application is connected with the speculation about an extended symmetric law of gravitation, based on the options of positive and negative mass for a particle at given positive energy. This would make masses of equal polarity attract each other, while masses of opposite polarity repel each other. Matter and antimatter are further proposed to be associated with the states of positive and negative mass. Under fully symmetric conditions this could provide a mechanism for the separation of antimatter from matter at an early stage of the universe.

  20. May 2002 Lidar Point Data of Southern California Coastline: Dana Point to Point La Jolla

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains lidar point data from a strip of Southern California coastline (including water, beach, cliffs, and top of cliffs) from Dana Point to Point La...

  1. September 2002 Lidar Point Data of Southern California Coastline: Dana Point to Point La Jolla

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains lidar point data from a strip of Southern California coastline (including water, beach, cliffs, and top of cliffs) from Dana Point to Point La...

  2. Learning power point 2000 easily

    Energy Technology Data Exchange (ETDEWEB)

    Mon, In Su; Je, Jung Suk

    2000-05-15

    This book introduces power point 2000, which gives descriptions what power point is, what we can do with power point 2000, is it possible to install power point 2000 in my computer? Let's run power point, basic of power point such as new presentation, writing letter, using text box, changing font size, color and shape, catching power user, insertion of word art and creating of new file. It also deals with figure, chart, graph, making multimedia file, presentation, know-how of power point for teachers and company workers.

  3. ATLAS Point 1 Construction

    CERN Multimedia

    Inigo-Golfin, J

    After 3 years of work in point 1, a number of surface buildings have already been completed and handed over to CERN (the control, the gas and the cooling and ventilation buildings) and, probably more appealing to the public, 60,000 m3 of earth have already been excavated from underground. At present, the technical cavern USA15 and its access shaft are almost finished, leaving only the main cavern and the liaison galleries to be completed in the coming year and a half. The main cavern has been excavated down to the radiation limit and its walls and vault will presently be concreted (see below the picture of the section of the vault with the impressive shell of 1.2 m thickness). The excavation of the bench (27 vertical metres to go yet!) will proceed from August, when some additional civil engineering work in the LHC tunnel will be undertaken. Needless to say many different services are necessary around the detector, both for its installation and future operation for physics. To that end much of the heavy...

  4. Point 1 Updates

    CERN Multimedia

    Inigo-Golfin, J.

    The ATLAS experimental area is located in Point 1, just across the main CERN entrance, in the commune of Meyrin. There people are ever so busy to finish the different infrastructures for ATLAS. Not only has Civil Engineering finished the construction of the USA15 technical cavern, but the excavation of the main UX15 cavern has resumed below the machine tunnel, after a brief halt to allow the construction of the UJ-caverns for the power converters of the LHC machine. The excavation work should end in August 2002. The UX15 hand-over to ATLAS is expected in April 2003. On the surface civil engineering is starting to complete the last two surface buildings (SDX1 and SH1), once the services (cooling pipes, ventilation ducts and the largest item, the lift modules and its lift of course) in the shaft PX15 have been completed. But the civil engineering is not all. A lot more is under way. The site installation of the steel structures in the caverns is to begin in Autumn, along with all the cooling pipes, airconditi...

  5. Rational points on varieties

    CERN Document Server

    Poonen, Bjorn

    2017-01-01

    This book is motivated by the problem of determining the set of rational points on a variety, but its true goal is to equip readers with a broad range of tools essential for current research in algebraic geometry and number theory. The book is unconventional in that it provides concise accounts of many topics instead of a comprehensive account of just one-this is intentionally designed to bring readers up to speed rapidly. Among the topics included are Brauer groups, faithfully flat descent, algebraic groups, torsors, étale and fppf cohomology, the Weil conjectures, and the Brauer-Manin and descent obstructions. A final chapter applies all these to study the arithmetic of surfaces. The down-to-earth explanations and the over 100 exercises make the book suitable for use as a graduate-level textbook, but even experts will appreciate having a single source covering many aspects of geometry over an unrestricted ground field and containing some material that cannot be found elsewhere. The origins of arithmetic (o...

  6. "Point de suspension"

    CERN Multimedia

    2004-01-01

    CERN - Globe of Science and Innovation 20 and 21 October Acrobatics, mime, a cappella singing, projections of images, a magical setting... a host of different tools of a grandeur matching that of the Universe they relate. A camera makes a massive zoom out to reveal the multiple dimensions of Nature. Freeze the frame: half way between the infinitesimally small and the infinitesimally large, a man suspends his everyday life (hence the title "Point de Suspension", which refers to the three dots at the end of an uncompleted sentence) to take a glimpse of the place he occupies in the great history of the Universe. An unusual perspective on what it means to be a human being... This wondrous show in the Globe of Science and Innovation, specially created by the Miméscope* company for the official ceremony marking CERN's fiftieth anniversary, is a gift from the Government of the Republic and Canton of Geneva, which also wishes to share this moment of wonder with the local population. There will be three perfo...

  7. "Point de suspension"

    CERN Multimedia

    2004-01-01

    http://www.cern.ch/cern50/ CERN - Globe of Science and Innovation 20 and 21 October Acrobatics, mime, a cappella singing, projections of images, a magical setting... a host of different tools of a grandeur matching that of the Universe they relate. A camera makes a massive zoom out to reveal the multiple dimensions of Nature. Freeze the frame: half way between the infinitesimally small and the infinitesimally large, a man suspends his everyday life (hence the title "Point de Suspension", which refers to the three dots at the end of an uncompleted sentence) to take a glimpse of the place he occupies in the great history of the Universe. An unusual perspective on what it means to be a human being... This wondrous show in the Globe of Science and Innovation, specially created by the Miméscope* company for the official ceremony marking CERN's fiftieth anniversary, is a gift from the Government of the Republic and Canton of Geneva, which also wishes to share this moment of wonder with the local pop...

  8. "Point de suspension"

    CERN Multimedia

    2004-01-01

    CERN - Globe of Science and Innovation 20 and 21 October Acrobatics, mime, a cappella singing, projections of images, a magical setting... a host of different tools of a grandeur matching that of the Universe they relate. A camera makes a massive zoom out to reveal the multiple dimensions of Nature. Freeze the frame: half way between the infinitesimally small and the infinitesimally large, a man suspends his everyday life (hence the title "Point de Suspension", which refers to the three dots at the end of an uncompleted sentence) to take a glimpse of the place he occupies in the great history of the Universe. An unusual perspective on what it means to be a human being... This spectacle in the Globe of Science and Innovation, specially created by the Miméscope* company for the official ceremony marking CERN's fiftieth anniversary, is a gift from the Government of the Republic and Canton of Geneva, which also wishes to share this moment of wonder with the local population. There will be three performances for...

  9. Curriculum Implementation: Adaptation to the IEP.

    Science.gov (United States)

    Novelli-Olmstead, Tina

    1980-01-01

    The article describes an overall approach used by model Ohio teachers to improve special education instruction for the hearing impaired through individualized education programing as required by P.L. 94-142 (the Education for All Handicapped Children Act). Sections address four program components: prescription, teaching, reassessment, and…

  10. Getting Involved in the IEP Process

    Science.gov (United States)

    Kowalski, Ellen; Lieberman, Lauren J.; Daggett, Sara

    2006-01-01

    Although, in many districts, physical educators are integral members of the Individualized Education Program (designed for students with disabilities such as Down syndrome and autism), in other districts, physical educators are only partially involved in the process or are not given the opportunity to be involved at all. However, the physical…

  11. Solving discrete zero point problems

    NARCIS (Netherlands)

    van der Laan, G.; Talman, A.J.J.; Yang, Z.F.

    2004-01-01

    In this paper an algorithm is proposed to .nd a discrete zero point of a function on the collection of integral points in the n-dimensional Euclidean space IRn.Starting with a given integral point, the algorithm generates a .nite sequence of adjacent integral simplices of varying dimension and

  12. PowerPoint 2010 Bible

    CERN Document Server

    Wempen, Faithe

    2010-01-01

    Master PowerPoint and improve your presentation skills-with one book!. It's no longer enough to have slide after slide of text, bullets, and charts. It's not even enough to have good speaking skills if your PowerPoint slides bore your audience. Get the very most out of all that PowerPoint 2010 has to offer while also learning priceless tips and techniques for making good presentations in this new PowerPoint 2010 Bible. Well-known PowerPoint expert and author Faithe Wempen provides formatting tips; shows you how to work with drawings, tables, and SmartArt; introduces new collaboration tools; wa

  13. Imaging study on acupuncture points

    Science.gov (United States)

    Yan, X. H.; Zhang, X. Y.; Liu, C. L.; Dang, R. S.; Ando, M.; Sugiyama, H.; Chen, H. S.; Ding, G. H.

    2009-09-01

    The topographic structures of acupuncture points were investigated by using the synchrotron radiation based Dark Field Image (DFI) method. Four following acupuncture points were studied: Sanyinjiao, Neiguan, Zusanli and Tianshu. We have found that at acupuncture point regions there exists the accumulation of micro-vessels. The images taken in the surrounding tissue out of the acupuncture points do not show such kind of structure. It is the first time to reveal directly the specific structure of acupuncture points by X-ray imaging.

  14. Imaging study on acupuncture points

    International Nuclear Information System (INIS)

    Yan, X H; Zhang, X Y; Liu, C L; Dang, R S; Ando, M; Sugiyama, H; Chen, H S; Ding, G H

    2009-01-01

    The topographic structures of acupuncture points were investigated by using the synchrotron radiation based Dark Field Image (DFI) method. Four following acupuncture points were studied: Sanyinjiao, Neiguan, Zusanli and Tianshu. We have found that at acupuncture point regions there exists the accumulation of micro-vessels. The images taken in the surrounding tissue out of the acupuncture points do not show such kind of structure. It is the first time to reveal directly the specific structure of acupuncture points by X-ray imaging.

  15. Magic Pointing for Eyewear Computers

    DEFF Research Database (Denmark)

    Jalaliniya, Shahram; Mardanbegi, Diako; Pederson, Thomas

    2015-01-01

    In this paper, we propose a combination of head and eye movements for touchlessly controlling the "mouse pointer" on eyewear devices, exploiting the speed of eye pointing and accuracy of head pointing. The method is a wearable computer-targeted variation of the original MAGIC pointing approach...... which combined gaze tracking with a classical mouse device. The result of our experiment shows that the combination of eye and head movements is faster than head pointing for far targets and more accurate than eye pointing....

  16. Improvement of calcium mineral separation contrast using anionic reagents: electrokinetics properties and flotation

    Science.gov (United States)

    Lafhaj, Z.; Filippov, L. O.; Filippova, I. V.

    2017-07-01

    The flotation separation of salt type calcium minerals is problematic, due to the similarities in their same active Ca2+ related site for interaction with anionic collectors and similar physicochemical characteristics such as solubility, zero-point charge, surface speciation and Ca-site density. The work was performed to achieve effective and selective separation of the calcium-minerals using pure minerals samples: orange calcite with Mg impurities, optic calcite with impurities level and an apatite. The pure samples surface was examined using techniques sensitive near-surface like infrared spectroscopy (FTIR) and chemical composition was obtained by ICPMS. The isoelectric point (IEP) and point of zero charge (PZC) in electrolyte were recorded using electrophoresis method at different ionic strengths of the solution. Mechanisms of charge development at the mineral-water interface are discussed. The time of contact as important parameter for the charge equilibrium was deduced from kinetics study and fixed to 30 minutes. The difference in the values obtained between IEP and PZSE can be explained by the presence of a specific adsorption of cations and anions on the surface. The effect of pure anionic collectors such as oleic and linoleic acid were studied. At low pH, both collectors lead to a good recovery for the calcites. The flotation recovery of optic calcite at pH 9 with sodium oleate is higher than with sodium linoleate. At alkaline pH, apatite showed a better recovery with sodium linoleate.

  17. Laser Dew-Point Hygrometer

    Science.gov (United States)

    Matsumoto, Shigeaki; Toyooka, Satoru

    1995-01-01

    A rough-surface-type automatic dew-point hygrometer was developed using a laser diode and an optical fiber cable. A gold plate with 0.8 µ m average surface roughness was used as a surface for deposition of dew to facilitate dew deposition and prevent supersaturation of water vapor at the dew point. It was shown experimentally that the quantity of dew deposited can be controlled to be constant at any predetermined level, and is independent of the dew point to be measured. The dew points were measured in the range from -15° C to 54° C in which the temperature ranged from 0° C to 60° C. The measurement error of the dew point was ±0.5° C which was equal to below ±2% in relative humidity in the above dew-point range.

  18. Fermat's point from five perspectives

    Science.gov (United States)

    Park, Jungeun; Flores, Alfinio

    2015-04-01

    The Fermat point of a triangle is the point such that minimizes the sum of the distances from that point to the three vertices. Five approaches to study the Fermat point of a triangle are presented in this article. First, students use a mechanical device using masses, strings and pulleys to study the Fermat point as the one that minimizes the potential energy of the system. Second, students use soap films between parallel planes connecting three pegs. The tension on the film will be minimal when the sum of distances is minimal. Third, students use an empirical approach, measuring distances in an interactive GeoGebra page. Fourth, students use Euclidean geometry arguments for two proofs based on the Torricelli configuration, and one using Viviani's Theorem. And fifth, the kinematic method is used to gain additional insight on the size of the angles between the segments joining the Fermat point with the vertices.

  19. The goal of ape pointing.

    Science.gov (United States)

    Halina, Marta; Liebal, Katja; Tomasello, Michael

    2018-01-01

    Captive great apes regularly use pointing gestures in their interactions with humans. However, the precise function of this gesture is unknown. One possibility is that apes use pointing primarily to direct attention (as in "please look at that"); another is that they point mainly as an action request (such as "can you give that to me?"). We investigated these two possibilities here by examining how the looking behavior of recipients affects pointing in chimpanzees (Pan troglodytes) and bonobos (Pan paniscus). Upon pointing to food, subjects were faced with a recipient who either looked at the indicated object (successful-look) or failed to look at the indicated object (failed-look). We predicted that, if apes point primarily to direct attention, subjects would spend more time pointing in the failed-look condition because the goal of their gesture had not been met. Alternatively, we expected that, if apes point primarily to request an object, subjects would not differ in their pointing behavior between the successful-look and failed-look conditions because these conditions differed only in the looking behavior of the recipient. We found that subjects did differ in their pointing behavior across the successful-look and failed-look conditions, but contrary to our prediction subjects spent more time pointing in the successful-look condition. These results suggest that apes are sensitive to the attentional states of gestural recipients, but their adjustments are aimed at multiple goals. We also found a greater number of individuals with a strong right-hand than left-hand preference for pointing.

  20. Anionic 11-mercaptoundecanoic acid capped ZnO nanoparticles

    Energy Technology Data Exchange (ETDEWEB)

    Šimšíková, Michaela, E-mail: michaela.simsikova@ceitec.vutbr.cz [CEITEC BUT, Brno University of Technology, Technická 10, 616 69 Brno (Czech Republic); Antalík, Marián [Department of Biochemistry, Faculty of Science, P.J. Šafárik University, Šrobárova 2, 041 54 Košice (Slovakia); Department of Biophysics, Institute of Experimental Physics, SAS, Watsonova 47, 040 01 Košice (Slovakia); Kaňuchová, Mária; Škvarla, Jiří [Institute of Montaneous Sciences and Environmental Protection, Faculty of Mining, Ecology, Process Control and Geotechnologies, Technical University of Košice, Park Komenského 19, 043 84 Košice (Slovakia)

    2013-10-01

    The anionic zinc oxide nanoparticles have been prepared at room temperature by a precipitation method using ZnCl{sub 2} and NaOH and surface modification with 11-mercaptoundecanoic acid (MUA). Atomic force microscopy (AFM) was used for definition of morphology and size of prepared nanoparticles which was proved by measurements of particle size distribution using Zetasizer. Successful coating with MUA as surfactant was acknowledged by X-ray photoelectron spectroscopy and ATR FT-IR spectroscopy. The isoelectric point (IEP) of ZnO–MUA nanoparticles was obtained by measurements of zeta potential and FT-IR dependence on pH; the obtained value was approximately 3.58. The value of exchanged protons was 2.88 which indicates a positive binding cooperativity of modified nanoparticles.

  1. Novel macroporous amphoteric gels: Preparation and characterization

    Directory of Open Access Journals (Sweden)

    S. Kudaibergenov

    2012-05-01

    Full Text Available Macroporous amphoteric gels based on allylamine, methacrylic acid and acrylamide crosslinked by N,N'-methylenebisacrylamide were synthesized by radical copolymerization of monomers in cryoconditions. The composition of cryogels was determined by combination of potentiometric and conductimetric titrations. The morphology of cryogels was evaluated by scanning electron microscope (SEM. Cryogels exhibited sponge-like porous structure with pore size ranging from 50 to 200 μm. The values of the isoelectric points (IEPs determined from the swelling experiments arranged between 3.5 and 4.3. The high adsorption-desorption capacity of amphoteric cryogels with respect to mM and trace concentrations of copper, nickel, and cobalt ions was demonstrated. It was shown that the macroporous amphoteric cryogels are able to adsorb up to 99.9% of copper, nickel, and cobalt ions from 10–3 mol•L–1 aqueous solution.

  2. AFM study of adsorption of protein A on a poly(dimethylsiloxane) surface

    International Nuclear Information System (INIS)

    Yu Ling; Lu Zhisong; Gan Ye; Liu Yingshuai; Li, C M

    2009-01-01

    In this paper, the morphology and kinetics of adsorption of protein A on a PDMS surface is studied by AFM. The results of effects of pH, protein concentration and contact time of the adsorption reveal that the morphology of adsorbed protein A is significantly affected by pH and adsorbed surface concentration, in which the pH away from the isoelectric point (IEP) of protein A could produce electrical repulsion to change the protein conformation, while the high adsorbed surface protein volume results in molecular networks. Protein A can form an adsorbed protein film on PDMS with a maximum volume of 2.45 x 10 -3 μm 3 . This work enhances our fundamental understanding of protein A adsorption on PDMS, a frequently used substrate component in miniaturized immunoassay devices.

  3. Tridodecylamine, an efficient charge control agent in non-polar media for electrophoretic inks application

    Science.gov (United States)

    Noel, Amélie; Mirbel, Déborah; Cloutet, Eric; Fleury, Guillaume; Schatz, Christophe; Navarro, Christophe; Hadziioannou, Georges; CyrilBrochon

    2018-01-01

    In order to obtain efficient electrophoretic inks, Tridodecylamine (Dod3N), has been studied as charge control agent (CCA) in a non-polar paraffin solvent (Isopar G) for various inorganic pigments (TiO2 and Fe2O3). All hydrophobic mineral oxides, i.e. treated with octyltrimethoxysilane (C8) or dodecyltrimethoxysilane (C12), were found to be negatively charged in presence of Dod3N. The electrophoretic mobilities of inorganic pigments seemed to be strongly dependent of their isoelectric point (IEP) and also of the concentration of dod3N with an optimum range between 10 and 20 mM depending on the pigments. Finally, an electrophoretic ink constituted of hydrophobic mineral oxides in presence of Dod3N was tested in a device. Its efficiency as charge control agent to negatively charge hydrophobic particles was confirmed through good optical properties and fast response time (220 ms at 200 kV m-1).

  4. Mathematical points as didactical ideas

    DEFF Research Database (Denmark)

    Mogensen, Arne

    Mathematics teaching in Denmark was recently recommended better organized in sequences with clear mathematical pedagogical goals and a focus on mathematical points. In this paper I define a mathematical point and inform on coding of transcripts in a video based Danish research study on grade 8 te...

  5. Review of NJ point system.

    Science.gov (United States)

    2013-03-01

    The purpose of this study is to investigate the comparative effectiveness of point-based versus incident-based : negligent driver monitoring systems and to explore how certain changes to the existing point-based system used in : New Jersey might impr...

  6. Heilbronn problem for five points

    International Nuclear Information System (INIS)

    Yang Lu; Zhang Jingzhong; Zeng Zhenbing

    1991-08-01

    Put 5 points in a unit square, then there must be at least one triangle, formed by three of these points, with an area not greater than √3/9. And the upper bound √3/9 is the best. Unexpectedly, this disproves an old conjecture posed twenty years ago. (author). 15 refs, 19 figs

  7. Model plant Key Measurement Points

    International Nuclear Information System (INIS)

    Schneider, R.A.

    1984-01-01

    For IAEA safeguards a Key Measurement Point is defined as the location where nuclear material appears in such a form that it may be measured to determine material flow or inventory. This presentation describes in an introductory manner the key measurement points and associated measurements for the model plant used in this training course

  8. Improved Dynamic Planar Point Location

    DEFF Research Database (Denmark)

    Brodal, Gerth Stølting; Arge, Lars; Georgiadis, Loukas

    2006-01-01

    We develop the first linear-space data structures for dynamic planar point location in general subdivisions that achieve logarithmic query time and poly-logarithmic update time.......We develop the first linear-space data structures for dynamic planar point location in general subdivisions that achieve logarithmic query time and poly-logarithmic update time....

  9. Cleaning Massive Sonar Point Clouds

    DEFF Research Database (Denmark)

    Arge, Lars Allan; Larsen, Kasper Green; Mølhave, Thomas

    2010-01-01

    We consider the problem of automatically cleaning massive sonar data point clouds, that is, the problem of automatically removing noisy points that for example appear as a result of scans of (shoals of) fish, multiple reflections, scanner self-reflections, refraction in gas bubbles, and so on. We...

  10. Endogenizing Prospect Theory's Reference Point

    OpenAIRE

    Ulrich Schmidt; Horst Zank

    2010-01-01

    In previous models of (cumulative) prospect theory reference-dependence of preferences is imposed beforehand and the location of the reference point is exogenously determined. This note provides a foundation of prospect theory, where reference-dependence is derived from preference conditions and a unique reference point arises endogenously.

  11. The Philosophy of Turning Points

    DEFF Research Database (Denmark)

    Turcan, Romeo V.

    2013-01-01

    business and management field, the turning point is seen as a valuable unit of analysis within the research field. It is expected that this paper will encourage a dynamic scholarly conversation about the concept of turning point and how it can aid international business researchers in the development...

  12. Geodetic Control Points - Multi-State Control Point Database

    Data.gov (United States)

    NSGIC State | GIS Inventory — The Multi-State Control Point Database (MCPD) is a database of geodetic and mapping control covering Idaho and Montana. The control were submitted by registered land...

  13. Making Sense of Boiling Points and Melting Points

    Indian Academy of Sciences (India)

    GENERAL | ARTICLE. The boiling and melting points of a pure substance are char- ... bonds, which involves high energy and hence high temperatures. Among the .... with zero intermolecular force at all temperatures and pressures, which ...

  14. NOTE: Do acupuncture points exist?

    Science.gov (United States)

    Yan, Xiaohui; Zhang, Xinyi; Liu, Chenglin; Dang, Ruishan; Huang, Yuying; He, Wei; Ding, Guanghong

    2009-05-01

    We used synchrotron x-ray fluorescence analysis to probe the distribution of four chemical elements in and around acupuncture points, two located in the forearm and two in the lower leg. Three of the four acupuncture points showed significantly elevated concentrations of elements Ca, Fe, Cu and Zn in relation to levels in the surrounding tissue, with similar elevation ratios for Cu and Fe. The mapped distribution of these elements implies that each acupuncture point seems to be elliptical with the long axis along the meridian.

  15. PowerPoint 2013 bible

    CERN Document Server

    Wempen, Faithe

    2013-01-01

    Master PowerPoint and improve your presentation skills with one book! In today's business climate, you need to know PowerPoint inside and out, and that's not all. You also need to be able to make a presentation that makes an impact. From using sophisticated transitions and animation in your PowerPoint presentations to interfacing in person with your audience, this information-packed book helps you succeed. Start creating professional-quality slides that captivate audiences and discover essential tips and techniques for making first-rate presentations, whether you're at a podium or

  16. Calorimetry end-point predictions

    International Nuclear Information System (INIS)

    Fox, M.A.

    1981-01-01

    This paper describes a portion of the work presently in progress at Rocky Flats in the field of calorimetry. In particular, calorimetry end-point predictions are outlined. The problems associated with end-point predictions and the progress made in overcoming these obstacles are discussed. The two major problems, noise and an accurate description of the heat function, are dealt with to obtain the most accurate results. Data are taken from an actual calorimeter and are processed by means of three different noise reduction techniques. The processed data are then utilized by one to four algorithms, depending on the accuracy desired to determined the end-point

  17. Hawaii ESI: NESTS (Nest Points)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains sensitive biological resource data for seabird nesting colonies in coastal Hawaii. Vector points in this data set represent locations of...

  18. Parametric statistical change point analysis

    CERN Document Server

    Chen, Jie

    2000-01-01

    This work is an in-depth study of the change point problem from a general point of view and a further examination of change point analysis of the most commonly used statistical models Change point problems are encountered in such disciplines as economics, finance, medicine, psychology, signal processing, and geology, to mention only several The exposition is clear and systematic, with a great deal of introductory material included Different models are presented in each chapter, including gamma and exponential models, rarely examined thus far in the literature Other models covered in detail are the multivariate normal, univariate normal, regression, and discrete models Extensive examples throughout the text emphasize key concepts and different methodologies are used, namely the likelihood ratio criterion, and the Bayesian and information criterion approaches A comprehensive bibliography and two indices complete the study

  19. Control Point Generated PLS - lines

    Data.gov (United States)

    Minnesota Department of Natural Resources — The Control Point Generated PLS layer contains line and polygon features to the 1/4 of 1/4 PLS section (approximately 40 acres) and government lot level. The layer...

  20. Detecting determinism from point processes.

    Science.gov (United States)

    Andrzejak, Ralph G; Mormann, Florian; Kreuz, Thomas

    2014-12-01

    The detection of a nonrandom structure from experimental data can be crucial for the classification, understanding, and interpretation of the generating process. We here introduce a rank-based nonlinear predictability score to detect determinism from point process data. Thanks to its modular nature, this approach can be adapted to whatever signature in the data one considers indicative of deterministic structure. After validating our approach using point process signals from deterministic and stochastic model dynamics, we show an application to neuronal spike trains recorded in the brain of an epilepsy patient. While we illustrate our approach in the context of temporal point processes, it can be readily applied to spatial point processes as well.

  1. Allegheny County Cell Tower Points

    Data.gov (United States)

    Allegheny County / City of Pittsburgh / Western PA Regional Data Center — This dataset portrays cell tower locations as points in Allegheny County. The dataset is based on outbuilding codes in the Property Assessment Parcel Database used...

  2. Virginia ESI: REPTPT (Reptile Points)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains sensitive biological resource data for sea turtles in Virginia. Vector points in this data set represent nesting sites. Species-specific...

  3. Maryland ESI: NESTS (Nest Points)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains sensitive biological resource data for raptors in Maryland. Vector points in this data set represent bird nesting sites. Species-specific...

  4. Processing Terrain Point Cloud Data

    KAUST Repository

    DeVore, Ronald; Petrova, Guergana; Hielsberg, Matthew; Owens, Luke; Clack, Billy; Sood, Alok

    2013-01-01

    Terrain point cloud data are typically acquired through some form of Light Detection And Ranging sensing. They form a rich resource that is important in a variety of applications including navigation, line of sight, and terrain visualization

  5. Control Point Generated PLS - polygons

    Data.gov (United States)

    Minnesota Department of Natural Resources — The Control Point Generated PLS layer contains line and polygon features to the 1/4 of 1/4 PLS section (approximately 40 acres) and government lot level. The layer...

  6. Calcareous Fens - Source Feature Points

    Data.gov (United States)

    Minnesota Department of Natural Resources — Pursuant to the provisions of Minnesota Statutes, section 103G.223, this database contains points that represent calcareous fens as defined in Minnesota Rules, part...

  7. Louisiana ESI: NESTS (Nest Points)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains sensitive biological resource data for seabird and wading bird nesting colonies in coastal Louisiana. Vector points in this data set represent...

  8. Some remarks on farthest points

    Czech Academy of Sciences Publication Activity Database

    Montesinos, V.; Zizler, P.; Zizler, Václav

    2011-01-01

    Roč. 105, č. 1 (2011), s. 119-131 ISSN 1578-7303 R&D Projects: GA AV ČR IAA100190901 Institutional research plan: CEZ:AV0Z10190503 Keywords : farthest points * strongly exposed points * generic differentiability * convex functions Subject RIV: BA - General Mathematics Impact factor: 0.340, year: 2011 http://link.springer.com/article/10.1007%2Fs13398-011-0012-z

  9. SharePoint User's Guide

    CERN Document Server

    Corporation, Infusion Development

    2009-01-01

    This straightforward guide shows SharePoint users how to create and use web sites for sharing and collaboration. Learn to use the document and picture libraries for adding and editing content, add discussion boards and surveys, receive alerts when documents and information have been added or changed, and enhance security. Designed to help you find answers quickly, the book shows how to make the most of SharePoint for productivity and collaboration.

  10. WHO Standard Acupuncture Point Locations

    Directory of Open Access Journals (Sweden)

    Sabina Lim

    2010-01-01

    Full Text Available ‘WHO Standard Acupuncture Point Locations in the Western Pacific Region (WHO Standard was released in 2008. Initially, there were 92/361 controversial acupuncture points (acupoints. Through seven informal consultations and four task force team meetings, 86 points were agreed upon among the 92 controversial acupoints, leaving 6 remaining controversial acupoints, demanding active research in the future. This will enhance the reproducibility and validity of acupuncture studies. It will also lead to a better understanding of acupuncture mechanisms in order to optimize its clinical efficacy for a range of diseases and syndromes. This book has two parts: General Guidelines for Acupuncture Point Locations and WHO Standard Acupuncture Point Locations. First of all, familiarity with the General Guidelines for Acupuncture Point Locations in this book can help the reader to understand and use the contents of this book in depth. I would like to thank all of the participating experts and scholars for this great work, who have overcome the limits of previous acupuncture references. I also appreciate the dedicated effort and harmonious leadership of Dr Choi Seung-hoon, former Regional Adviser in Traditional Medicine of Western Pacific Office, WHO.

  11. Critical points in magnetic systems

    International Nuclear Information System (INIS)

    Bongaarts, A.L.M.

    1975-01-01

    The magnetical phase transitions of CsCoCl 3 .2H 2 O and CsCoCl 3 .2D 2 O are investigated by neutron diffraction techniques with special attention to the critical points in the phase diagrams. CsCoCl 3 .2H 2 O turned out to be a one-dimentional magnetic antiferromagnet with ferromagnetic and antiferromagnetic interactions. In the vicinity of the Neel point, the critical behavior in zero magnetic field could be described as a three-dimentional long range ordering, while the fluctuations in the system are one-dimensional. In the presence of a magnetic field, the behavior of the system in the critical region of the magnetic phase diagram between the Neel temperature at zero field (3.3degK) and 1.85degK, was in good agreement with the theory. Below 1.85degK, the phase transition in a magnetic field changes into a line of triple points whose end point could be identified as a tricritical point, i.e., an intersection of three critical lines. The parameters derived from observations in the neighborhood of this tricritical point obey the scaling laws but are not in numerical agreement with theoretical predictions

  12. Recent progress in fission at saddle point and scission point

    International Nuclear Information System (INIS)

    Blons, J.; Paya, D.; Signarbieux, C.

    High resolution measurements of 230 Th and 232 Th fission cross sections for neutrons exhibit a fine structure. Such a structure is interpreted as a superposition of two rotational bands in the third, asymmetric, well of the fission barrier. The fragment mass distribution in the thermal fission of 235 U and 233 U does not show any even-odd effect, even at the highest kinetic energies. This is the mark of a strong viscosity in the descent from saddle point to scission point [fr

  13. Digital microwave communication engineering point-to-point microwave systems

    CERN Document Server

    Kizer, George

    2013-01-01

    The first book to cover all engineering aspects of microwave communication path design for the digital age Fixed point-to-point microwave systems provide moderate-capacity digital transmission between well-defined locations. Most popular in situations where fiber optics or satellite communication is impractical, it is commonly used for cellular or PCS site interconnectivity where digital connectivity is needed but not economically available from other sources, and in private networks where reliability is most important. Until now, no book has adequately treated all en

  14. March 2003 Lidar Point Data of Southern California Coastline: Dana Point to Point La Jolla

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains lidar point data (Geodetic Coordinates) from a strip of Southern California coastline (including water, beach, cliffs, and top of cliffs) from...

  15. April 2004 Lidar Point Data of Southern California Coastline: Dana Point to Point La Jolla

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains lidar point data (Geodetic Coordinates) from a strip of Southern California coastline (including water, beach, cliffs, and top of cliffs) from...

  16. December 2002 Lidar Point Data of Southern California Coastline: Dana Point to Point La Jolla

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains lidar point data (latitude and longitude) from a strip of Southern California coastline (including water, beach, cliffs, and top of cliffs)...

  17. Dynamic magnetic x-points

    International Nuclear Information System (INIS)

    Leboeuf, J.N.; Tajima, T.; Dawson, J.M.

    1981-03-01

    Two-and-one-half dimensional magnetostatic and electromagnetic particle simulations of time-varying magnetic x-points and the associated plasma response are reported. The stability and topology depend on the crossing angle of the field lines at the x-point, irrespective of the plasma β. The electrostatic field and finite Larmor radius effects play an important role in current penetration and shaping of the plasma flow. The snapping of the field lines, and dragging of the plasma into, and confinement of the plasma at, an o-point (magnetic island) is observed. Magnetic island coalescence with explosive growth of the coalescence mode occurs and is accompanied by a large increase of kinetic energy and temperature as well as the formation of hot tails on the distribution functions

  18. Fixed points of quantum operations

    International Nuclear Information System (INIS)

    Arias, A.; Gheondea, A.; Gudder, S.

    2002-01-01

    Quantum operations frequently occur in quantum measurement theory, quantum probability, quantum computation, and quantum information theory. If an operator A is invariant under a quantum operation φ, we call A a φ-fixed point. Physically, the φ-fixed points are the operators that are not disturbed by the action of φ. Our main purpose is to answer the following question. If A is a φ-fixed point, is A compatible with the operation elements of φ? We shall show in general that the answer is no and we shall give some sufficient conditions under which the answer is yes. Our results will follow from some general theorems concerning completely positive maps and injectivity of operator systems and von Neumann algebras

  19. Fixed-point signal processing

    CERN Document Server

    Padgett, Wayne T

    2009-01-01

    This book is intended to fill the gap between the ""ideal precision"" digital signal processing (DSP) that is widely taught, and the limited precision implementation skills that are commonly required in fixed-point processors and field programmable gate arrays (FPGAs). These skills are often neglected at the university level, particularly for undergraduates. We have attempted to create a resource both for a DSP elective course and for the practicing engineer with a need to understand fixed-point implementation. Although we assume a background in DSP, Chapter 2 contains a review of basic theory

  20. Implementing floating-point DSP

    Czech Academy of Sciences Publication Activity Database

    Kadlec, Jiří; Chappel, S.

    2006-01-01

    Roč. 2, č. 3 (2006), s. 12-14 R&D Projects: GA AV ČR 1ET400750406; GA MŠk 1M0567 EU Projects: European Commission(XE) 027611 - AETHER Program:FP6 Institutional research plan: CEZ:AV0Z10750506 Keywords : PicoBlaze * floating point * FPGA Subject RIV: JC - Computer Hardware ; Software http://www.xilinx.com/publications/ magazines /emb_03/xc_pdf/p12-14_3emb-point.pdf

  1. Kinetic and Surface Study of Single-Walled Aluminosilicate Nanotubes and Their Precursors

    Directory of Open Access Journals (Sweden)

    Mauricio Molina

    2013-03-01

    Full Text Available The structural and surface changes undergone by the different precursors that are produced during the synthesis of imogolite are reported. The surface changes that occur during the synthesis of imogolite were determined by electrophoretic migration (EM measurements, which enabled the identification of the time at which the critical precursor of the nanoparticles was generated. A critical parameter for understanding the evolution of these precursors is the isoelectric point (IEP, of which variation revealed that the precursors modify the number of active ≡Al-OH and ≡Si-OH sites during the formation of imogolite. We also found that the IEP is displaced to a higher pH level as a consequence of the surface differentiation that occurs during the synthesis. At the same time, we established that the pH of the reaction (pHrx decreases with the evolution and condensation of the precursors during aging. Integration of all of the obtained results related to the structural and surface properties allows an overall understanding of the different processes that occur and the products that are formed during the synthesis of imogolite.

  2. Sodium caseinate stabilized zein colloidal particles.

    Science.gov (United States)

    Patel, Ashok R; Bouwens, Elisabeth C M; Velikov, Krassimir P

    2010-12-08

    The present work deals with the preparation and stabilization of zein colloidal particles using sodium caseinate as electrosteric stabilizer. Colloidal particles with well-defined size range (120-150 nm) and negative surface potential (-29 to -47 mV) were obtained using a simple antisolvent precipitation method. Due to the presence of caseinate, the stabilized colloidal particles showed a shift of isoelectric point (IEP) from 6.0 to around pH 5.0 and thus prevent the aggregation of zein near its native IEP (pH 6.2). The particles also showed good stability to varying ionic strength (15 mM-1.5 M NaCl). Furthermore, stabilized particles retained the property of redispersibility after drying. In vitro protein hydrolysis study confirmed that the presence of caseinate did not alter the digestibility of zein. Such colloidal particles could potentially serve as all-natural delivery systems for bioactive molecules in food, pharmaceutical, and agricultural formulations.

  3. Nuclear waste treatment - Studying the mixed ion type effects and concentration on the behaviour of oxide dispersions

    International Nuclear Information System (INIS)

    Omokanye, Qanitalillahi; Biggs, Simon

    2007-01-01

    In order to gain good control over a particulate dispersion it is necessary to accurately characterise the strength of inter-particle forces that may be operating. Such control is not routinely used, as yet, in the nuclear industry despite the possible benefits. We are investigating the impact of mixed electrolyte systems, for example NaCl and Na 2 SO 4 , on the stability of oxide simulant particle dispersions. The electro-acoustic zeta potentials and shear yield stresses for concentrated dispersions have been measured across a range of pH conditions and electrolyte concentrations (0.001 M - 1.0 M). This paper summarizes initial data from these studies showing how the shear yield stress of concentrated aqueous oxide particle dispersions, can be adjusted through regulation of pH and the addition of background electrolytes (salt). The yield stress as a function of pH for these dispersions in mixed electrolytes showed a direct correlation with corresponding measurements of the zeta potential. Changes in the background electrolyte concentration or type were seen to cause a shift in the position of the isoelectric point (iep). Measurements of the shear yield stress showed a maximum at the iep corresponding to the position of maximum instability in the suspension. The consequences of these data for the efficient treatment of solid-liquid systems will be discussed. (authors)

  4. Heterocoagulation of chalcopyrite and pyrite minerals in flotation separation.

    Science.gov (United States)

    Mitchell, Timothy K; Nguyen, Anh V; Evans, Geoffrey M

    2005-06-30

    Heterocoagulation between various fine mineral particles contained within a mineral suspension with different structural and surface chemistry can interfere with the ability of the flotation processes to selectively separate the minerals involved. This paper examines the interactions between chalcopyrite (a copper mineral) and pyrite (an iron mineral often bearing gold) as they approach each other in suspensions with added chemicals, and relates the results to the experimental data for the flotation recovery and selectivity. The heterocoagulation was experimentally studied using the electrophoretic light scattering (ELS) technique and was modelled by incorporating colloidal forces, including the van der Waals, electrostatic double layer and hydrophobic forces. The ELS results indicated that pyrite has a positive zeta potential (zeta) up to its isoelectric point (IEP) at approximately pH 2.2, while chalcopyrite has a positive zeta up to its IEP at approximately pH 5.5. This produces heterocoagulation of chalcopyrite with pyrite between pH 2.2 and pH 5.5. The heterocoagulation was confirmed by the ELS spectra measured with a ZetaPlus instrument from Brookhaven and by small-scale flotation experiments.

  5. The role of poly(methacrylic acid) conformation on dispersion behavior of nano TiO2 powder

    Science.gov (United States)

    Singh, Bimal P.; Nayak, Sasmita; Samal, Samata; Bhattacharjee, Sarama; Besra, Laxmidhar

    2012-02-01

    To exploit the advantages of nanoparticles for various applications, controlling the dispersion and agglomeration is of paramount importance. Agglomeration and dispersion behavior of titanium dioxide (TiO2) nanoparticles was investigated using electrokinetic and surface chemical properties. Nanoparticles are generally stabilized by the adsorption of a dispersant (polyelectrolyte) layer around the particle surface and in this connection ammonium salt of polymethacrylic acid (Darvan C) was used as dispersant to stabilize the suspension. The dosages of polyelectrolyte were optimized to get best dispersion stability by techniques namely particle charge detector (13.75 mg/g) and adsorption (14.57 mg/g). The surface charge of TiO2 particles changed significantly in presence of dispersant Darvan C and isoelectric point (iep) shifted significantly towards lower pH from 5.99 to 3.37. The shift in iep has been quantified in terms of free energy of interaction between the surface sites of TiO2 and the adsorbing dispersant Darvan C. Free energies of adsorption were calculated by electrokinetic data (-9.8 RT unit) and adsorption isotherms (-10.56 RT unit), which corroborated well. The adsorption isotherms are of typical Langmuir type and employed for calculation of free energy. The results indicated that adsorption occurs mainly through electrostatic interactions between the dispersant molecule and the TiO2 surface apart from hydrophobic interactions.

  6. Synthesis and surface properties of submicron barium sulfate particles

    International Nuclear Information System (INIS)

    Zhang Ming; Zhang Bao; Li Xinhai; Yin Zhoulan; Guo Xueyi

    2011-01-01

    Barium sulfate particles were synthesized in the presence of EDTA at room temperature. X-ray diffractometry (XRD), Fourier transform infrared resonance (FTIR) and scanning electron microscopy (SEM) were used to characterize the structure and morphology of BaSO 4 particles. The effect of the preparation parameters on the particle size distribution and morphology was investigated. The conditional formation constants of Ba-EDTA at different pH values were calculated. The results show that the size and morphology of BaSO 4 particles can be effectively controlled by adding EDTA in the precipitation process. Among all the operation conditions, the pH value has significant effect on the particle size. The obtained barium sulfate particles are spherical and well dispersed at pH = 9-10. Zeta potentials of BaSO 4 were measured at different pH. The isoelectric point (IEP) of barium sulfate colloid appears at pH 6.92. The model of the solid-solution interface at a particle of BaSO 4 was presented. The FTIR result indicates that the surface of the prepared BaSO 4 absorbs the functional groups of EDTA, which lower the IEP of the barium sulfate particles.

  7. Synthesis and surface properties of submicron barium sulfate particles

    Energy Technology Data Exchange (ETDEWEB)

    Zhang Ming, E-mail: csu.light@yahoo.com.cn [College of Chemistry and Chemical Engineering, Zhaoqing University, Zhaoqing 526061 (China); School of Chemistry and Chemical Engineering, Central South University, Changsha 410083 (China); Zhang Bao; Li Xinhai [School of Metallurgical Science and Engineering, Central South University, Changsha 410083 (China); Yin Zhoulan [School of Chemistry and Chemical Engineering, Central South University, Changsha 410083 (China); Guo Xueyi [School of Metallurgical Science and Engineering, Central South University, Changsha 410083 (China)

    2011-10-15

    Barium sulfate particles were synthesized in the presence of EDTA at room temperature. X-ray diffractometry (XRD), Fourier transform infrared resonance (FTIR) and scanning electron microscopy (SEM) were used to characterize the structure and morphology of BaSO{sub 4} particles. The effect of the preparation parameters on the particle size distribution and morphology was investigated. The conditional formation constants of Ba-EDTA at different pH values were calculated. The results show that the size and morphology of BaSO{sub 4} particles can be effectively controlled by adding EDTA in the precipitation process. Among all the operation conditions, the pH value has significant effect on the particle size. The obtained barium sulfate particles are spherical and well dispersed at pH = 9-10. Zeta potentials of BaSO{sub 4} were measured at different pH. The isoelectric point (IEP) of barium sulfate colloid appears at pH 6.92. The model of the solid-solution interface at a particle of BaSO{sub 4} was presented. The FTIR result indicates that the surface of the prepared BaSO{sub 4} absorbs the functional groups of EDTA, which lower the IEP of the barium sulfate particles.

  8. Estudo da adsorção de dispersante à base de poliacrilato em um sistema varistor comercial: caracterização físico-química Study of polyacrylate adsorption in a commercial varistor system: characterization of the physical-chemistry properties

    Directory of Open Access Journals (Sweden)

    S. L. M. Brito

    2005-03-01

    Full Text Available Um dos grandes desafios no processamento cerâmico é a obtenção de dispersões de partículas estáveis e homogêneas contendo materiais com diferentes características físicas e químicas, como densidade, área de superfície específica, distribuição de tamanho de partículas e ponto isoelétrico. O comportamento de suspensões obtidas a partir da composição de um sistema varistor à base de ZnO (aditivos: Bi2O3, Sb2O3, Co3O4, Mn2O3, NiO, Cr2O3 em meio aquoso, utilizando o poliacrilato de amônio como dispersante foi avaliada para determinar a influência dos diferentes óxidos no processamento. Os óxidos foram caracterizados a partir das técnicas mobilidade eletroforética dinâmica, espectroscopia no infravermelho, distribuição granulométrica e área de superfície específica. Os óxidos aditivos apresentam características de superfície, como ponto isoelétricos (pie, distintos com relação ao óxido base (ZnO - pie 9.4. Os dois mais importantes, devido às fortes diferenças de pie foram o Sb2O3 e o Cr2O3, pie = 3,4 e 4,4, respectivamente. Esta diferença de pie entre o ZnO e os aditivos promovem uma mudança no pH quando em dispersão aquosa, que passa a ser de aproximadamente 8,0 e que pode comprometer a estabilidade da dispersão em algumas etapas do processo de dispersão.One of the greatest challenges of ceramic processing is to obtain a stable and homogeneous dispersion of particles when the components present different physical and chemical characteristics, such as density, specific surface area, size distribution and isoelectric point (iep. The ZnO varistor composition contains 7 different oxides (additives: Bi2O3, Sb2O3, Co3O4, Mn2O3, NiO, and Cr2O3 and the stability of the aqueous dispersion strongly depends on the specific surface area, the iep and the dispersant choice. In this work we evaluated the ammonium polyacrylate (PAA-NH4 as a dispersant to determine the influence of the different oxides on the

  9. Beyond the Point Ps Approximation

    OpenAIRE

    Stepanov, Sergey V.; Zvezhinskiy, Dmitry S.; Byakov, Vsevolod M.

    2012-01-01

    In application to positron annihilation spectroscopy, Ps atom is considered not as a point particle, but as a finite size e+ e- pair localized in a bubble-state in a medium. Variation of the internal Coulombic e+ -e- attraction vs. the bubble radius is estimated.

  10. Smooth random change point models.

    Science.gov (United States)

    van den Hout, Ardo; Muniz-Terrera, Graciela; Matthews, Fiona E

    2011-03-15

    Change point models are used to describe processes over time that show a change in direction. An example of such a process is cognitive ability, where a decline a few years before death is sometimes observed. A broken-stick model consists of two linear parts and a breakpoint where the two lines intersect. Alternatively, models can be formulated that imply a smooth change between the two linear parts. Change point models can be extended by adding random effects to account for variability between subjects. A new smooth change point model is introduced and examples are presented that show how change point models can be estimated using functions in R for mixed-effects models. The Bayesian inference using WinBUGS is also discussed. The methods are illustrated using data from a population-based longitudinal study of ageing, the Cambridge City over 75 Cohort Study. The aim is to identify how many years before death individuals experience a change in the rate of decline of their cognitive ability. Copyright © 2010 John Wiley & Sons, Ltd.

  11. The Mean as Balance Point

    Science.gov (United States)

    O'Dell, Robin S.

    2012-01-01

    There are two primary interpretations of the mean: as a leveler of data (Uccellini 1996, pp. 113-114) and as a balance point of a data set. Typically, both interpretations of the mean are ignored in elementary school and middle school curricula. They are replaced with a rote emphasis on calculation using the standard algorithm. When students are…

  12. Equations for arithmetic pointed tori

    NARCIS (Netherlands)

    Sijsling, J.R.

    2010-01-01

    In 1983, Kisao Takeuchi enumerated all 71 arithmetic (1;e)-groups. This is a special set of discrete subgroups of SL(2,R) of finite covolume and signature (1;e). The corresponding quotients of the upper half plane (called (1;e)-curves) have genus equal to 1 and a single elliptic point of order e.

  13. Flat Coalgebraic Fixed Point Logics

    Science.gov (United States)

    Schröder, Lutz; Venema, Yde

    Fixed point logics are widely used in computer science, in particular in artificial intelligence and concurrency. The most expressive logics of this type are the μ-calculus and its relatives. However, popular fixed point logics tend to trade expressivity for simplicity and readability, and in fact often live within the single variable fragment of the μ-calculus. The family of such flat fixed point logics includes, e.g., CTL, the *-nesting-free fragment of PDL, and the logic of common knowledge. Here, we extend this notion to the generic semantic framework of coalgebraic logic, thus covering a wide range of logics beyond the standard μ-calculus including, e.g., flat fragments of the graded μ-calculus and the alternating-time μ-calculus (such as ATL), as well as probabilistic and monotone fixed point logics. Our main results are completeness of the Kozen-Park axiomatization and a timed-out tableaux method that matches ExpTime upper bounds inherited from the coalgebraic μ-calculus but avoids using automata.

  14. Fixed points of quantum gravity

    OpenAIRE

    Litim, D F

    2003-01-01

    Euclidean quantum gravity is studied with renormalisation group methods. Analytical results for a non-trivial ultraviolet fixed point are found for arbitrary dimensions and gauge fixing parameter in the Einstein-Hilbert truncation. Implications for quantum gravity in four dimensions are discussed.

  15. ACS Photometric Zero Point Verification

    Science.gov (United States)

    Dolphin, Andrew

    2003-07-01

    The uncertainties in the photometric zero points create a fundamental limit to the accuracy of photometry. The current state of the ACS calibration is surprisingly poor, with zero point uncertainties of 0.03 magnitudes in the Johnson filters. The reason for this is that ACS observations of excellent ground-based standard fields, such as the omega Cen field used for WFPC2 calibrations, have not been obtained. Instead, the ACS photometric calibrations are based primarily on semi-emprical synthetic zero points and observations of fields too crowded for accurate ground-based photometry. I propose to remedy this problem by obtaining ACS broadband images of the omega Cen standard field with both the WFC and HRC. This will permit the direct determination of the ACS transformations, and is expected to double the accuracy to which the ACS zero points are known. A second benefit is that it will facilitate the comparison of the WFPC2 and ACS photometric systems, which will be important as WFPC2 is phased out and ACS becomes HST's primary imager.

  16. Determine point-to-point networking interactions using regular expressions

    Directory of Open Access Journals (Sweden)

    Konstantin S. Deev

    2015-06-01

    Full Text Available As Internet growth and becoming more popular, the number of concurrent data flows start to increasing, which makes sense in bandwidth requested. Providers and corporate customers need ability to identify point-to-point interactions. The best is to use special software and hardware implementations that distribute the load in the internals of the complex, using the principles and approaches, in particular, described in this paper. This paper represent the principles of building system, which searches for a regular expression match using computing on graphics adapter in server station. A significant computing power and capability to parallel execution on modern graphic processor allows inspection of large amounts of data through sets of rules. Using the specified characteristics can lead to increased computing power in 30…40 times compared to the same setups on the central processing unit. The potential increase in bandwidth capacity could be used in systems that provide packet analysis, firewalls and network anomaly detectors.

  17. Modelling point patterns with linear structures

    DEFF Research Database (Denmark)

    Møller, Jesper; Rasmussen, Jakob Gulddahl

    2009-01-01

    processes whose realizations contain such linear structures. Such a point process is constructed sequentially by placing one point at a time. The points are placed in such a way that new points are often placed close to previously placed points, and the points form roughly line shaped structures. We...... consider simulations of this model and compare with real data....

  18. Modelling point patterns with linear structures

    DEFF Research Database (Denmark)

    Møller, Jesper; Rasmussen, Jakob Gulddahl

    processes whose realizations contain such linear structures. Such a point process is constructed sequentially by placing one point at a time. The points are placed in such a way that new points are often placed close to previously placed points, and the points form roughly line shaped structures. We...... consider simulations of this model and compare with real data....

  19. Zero Point of Historical Time

    Directory of Open Access Journals (Sweden)

    R.S. Khakimov

    2014-02-01

    Full Text Available Historical studies are based on the assumption that there is a reference-starting point of the space-time – the Zero point of coordinate system. Due to the bifurcation in the Zero Point, the course of social processes changes sharply and the probabilistic causality replaces the deterministic one. For this reason, changes occur in the structure of social relations and statehood form as well as in the course of the ethnic processes. In such a way emerges a new discourse of the national behavior. With regard to the history of the Tatars and Tatarstan, such bifurcation points occurred in the periods of the formation: 1 of the Turkic Khaganate, which began to exist from the 6th century onward and became a qualitatively new State system that reformatted old elements in the new matrix introducing a new discourse of behavior; 2 of the Volga-Kama Bulgaria, where the rivers (Kama, Volga, Vyatka became the most important trade routes determining the singularity of this State. Here the nomadic culture was connected with the settled one and Islam became the official religion in 922; 3 and of the Golden Hordе, a powerful State with a remarkable system of communication, migration of huge human resources for thousands of kilometers, and extensive trade, that caused severe “mutations” in the ethnic terms and a huge mixing of ethnic groups. Given the dwelling space of Tatar population and its evolution within Russia, it can be argued that the Zero point of Tatar history, which conveyed the cultural invariants until today, begins in the Golden Horde. Neither in the Turkic khaganate nor in the Bulgar State, but namely in the Golden Horde. Despite the radical changes, the Russian Empire failed to transform the Tatars in the Russians. Therefore, contemporary Tatars preserved the Golden Horde tradition as a cultural invariant.

  20. Characterization of MIPAS elevation pointing

    Directory of Open Access Journals (Sweden)

    M. Kiefer

    2007-01-01

    Full Text Available Sufficient knowledge of the pointing is essential for analyses of limb emission measurements. The scientific retrieval processor for MIPAS on ENVISAT operated at IMK allows the retrieval of pointing information in terms of tangent altitudes along with temperature. The retrieved tangent altitudes are independent of systematic offsets in the engineering Line-Of-Sight (LOS information delivered with the ESA Level 1b product. The difference of pointing retrieved from the reprocessed high resolution MIPAS spectra and the engineering pointing information was examined with respect to spatial/temporal behaviour. Among others the following characteristics of MIPAS pointing could be identified: Generally the engineering tangent altitudes are too high by 0–1.8 km with conspicuous variations in this range over time. Prior to December of 2003 there was a drift of about 50–100 m/h, which was due to a slow change in the satellite attitude. A correction of this attitude is done twice a day, which leads to discontinuities in the order of 1–1.5 km in the tangent altitudes. Occasionally discontinuities up to 2.5 km are found, as already reported from MIPAS and SCIAMACHY observations. After an update of the orbit position software in December 2003 values of drift and jumps are much reduced. There is a systematic difference in the mispointing between the poles which amounts to 1.5–2 km, i.e. there is a conspicuous orbit-periodic feature. The analysis of the correlation between the instrument's viewing angle azimuth and differential mispointing supports the hypotheses that a major part of this latter phenomenon can be attributed to an error in the roll angle of the satellite/instrument system of approximately 42 mdeg. One conclusion is that ESA level 2 data should be compared to other data exclusively on tangent pressure levels. Complementary to IMK data, ESA operational LOS calibration results were used to characterize MIPAS pointing. For this purpose

  1. Femtosecond photoelectron point projection microscope

    International Nuclear Information System (INIS)

    Quinonez, Erik; Handali, Jonathan; Barwick, Brett

    2013-01-01

    By utilizing a nanometer ultrafast electron source in a point projection microscope we demonstrate that images of nanoparticles with spatial resolutions of the order of 100 nanometers can be obtained. The duration of the emission process of the photoemitted electrons used to make images is shown to be of the order of 100 fs using an autocorrelation technique. The compact geometry of this photoelectron point projection microscope does not preclude its use as a simple ultrafast electron microscope, and we use simple analytic models to estimate temporal resolutions that can be expected when using it as a pump-probe ultrafast electron microscope. These models show a significant increase in temporal resolution when comparing to ultrafast electron microscopes based on conventional designs. We also model the microscopes spectroscopic abilities to capture ultrafast phenomena such as the photon induced near field effect

  2. Sensing with Superconducting Point Contacts

    Directory of Open Access Journals (Sweden)

    Argo Nurbawono

    2012-05-01

    Full Text Available Superconducting point contacts have been used for measuring magnetic polarizations, identifying magnetic impurities, electronic structures, and even the vibrational modes of small molecules. Due to intrinsically small energy scale in the subgap structures of the supercurrent determined by the size of the superconducting energy gap, superconductors provide ultrahigh sensitivities for high resolution spectroscopies. The so-called Andreev reflection process between normal metal and superconductor carries complex and rich information which can be utilized as powerful sensor when fully exploited. In this review, we would discuss recent experimental and theoretical developments in the supercurrent transport through superconducting point contacts and their relevance to sensing applications, and we would highlight their current issues and potentials. A true utilization of the method based on Andreev reflection analysis opens up possibilities for a new class of ultrasensitive sensors.

  3. Leverage points for sustainability transformation.

    Science.gov (United States)

    Abson, David J; Fischer, Joern; Leventon, Julia; Newig, Jens; Schomerus, Thomas; Vilsmaier, Ulli; von Wehrden, Henrik; Abernethy, Paivi; Ives, Christopher D; Jager, Nicolas W; Lang, Daniel J

    2017-02-01

    Despite substantial focus on sustainability issues in both science and politics, humanity remains on largely unsustainable development trajectories. Partly, this is due to the failure of sustainability science to engage with the root causes of unsustainability. Drawing on ideas by Donella Meadows, we argue that many sustainability interventions target highly tangible, but essentially weak, leverage points (i.e. using interventions that are easy, but have limited potential for transformational change). Thus, there is an urgent need to focus on less obvious but potentially far more powerful areas of intervention. We propose a research agenda inspired by systems thinking that focuses on transformational 'sustainability interventions', centred on three realms of leverage: reconnecting people to nature, restructuring institutions and rethinking how knowledge is created and used in pursuit of sustainability. The notion of leverage points has the potential to act as a boundary object for genuinely transformational sustainability science.

  4. EBTR design-point selection

    International Nuclear Information System (INIS)

    Krakowski, R.A.; Bathke, C.G.

    1981-01-01

    The procedure used to select the design point for the ELMO Bumpy Torus Reactor (EBTR) study is described. The models used in each phase of the selection process are described, with an emphasis placed on the parametric design curves produced by each model. The tradeoffs related to burn physics, stability/equilibrium, electron-ring physics, and magnetics design are discussed. The resulting design point indicates a plasma with a 35-m major radius and a 1-m minor radium operating at an average core-plasma beta of 0.17, which at approx. 30 keV produces an average neutron wall loading of 1.4 MW/m 2 while maintaining key magnet (< 10 T) and total power (less than or equal to 4000 MWt) constraints

  5. The point of 6 sigma

    International Nuclear Information System (INIS)

    An, Yeong Jin

    2000-07-01

    This book gives descriptions of the point of 6 sigma. These are the titles of this : what 6 sigma is, sigma conception, motor roller 3.4 ppm, centering error, 6 sigma purpose, 6 sigma principle, eight steps of innovation strategy, 6 sigma innovation strategy of easy system step, measurement standard of 6 sigma outcome, the main role of 6 sigma, acknowledgment and reword, 6 sigma characteristic, 6 sigma effect, 6 sigma application and problems which happen when 6 sigma introduces.

  6. Change point analysis and assessment

    DEFF Research Database (Denmark)

    Müller, Sabine; Neergaard, Helle; Ulhøi, John Parm

    2011-01-01

    The aim of this article is to develop an analytical framework for studying processes such as continuous innovation and business development in high-tech SME clusters that transcends the traditional qualitative-quantitative divide. It integrates four existing and well-recognized approaches...... to studying events, processes and change, mamely change-point analysis, event-history analysis, critical-incident technique and sequence analysis....

  7. Turning points in reactor design

    International Nuclear Information System (INIS)

    Beckjord, E.S.

    1995-01-01

    This article provides some historical aspects on nuclear reactor design, beginning with PWR development for Naval Propulsion and the first commercial application at Yankee Rowe. Five turning points in reactor design and some safety problems associated with them are reviewed: (1) stability of Dresden-1, (2) ECCS, (3) PRA, (4) TMI-2, and (5) advanced passive LWR designs. While the emphasis is on the thermal-hydraulic aspects, the discussion is also about reactor systems

  8. Turning points in reactor design

    Energy Technology Data Exchange (ETDEWEB)

    Beckjord, E.S.

    1995-09-01

    This article provides some historical aspects on nuclear reactor design, beginning with PWR development for Naval Propulsion and the first commercial application at Yankee Rowe. Five turning points in reactor design and some safety problems associated with them are reviewed: (1) stability of Dresden-1, (2) ECCS, (3) PRA, (4) TMI-2, and (5) advanced passive LWR designs. While the emphasis is on the thermal-hydraulic aspects, the discussion is also about reactor systems.

  9. Viviani Polytopes and Fermat Points

    Science.gov (United States)

    Zhou, Li

    2012-01-01

    Given a set of oriented hyperplanes P = {p1, . . . , pk} in R[superscript n], define v : R[superscript n] [right arrow] R by v(X) = the sum of the signed distances from X to p[subscript 1], . . . , p[subscript k], for any point X [is a member of] R[superscript n]. We give a simple geometric characterization of P for which v is constant, leading to…

  10. A fixed-point farrago

    CERN Document Server

    Shapiro, Joel H

    2016-01-01

    This text provides an introduction to some of the best-known fixed-point theorems, with an emphasis on their interactions with topics in analysis. The level of exposition increases gradually throughout the book, building from a basic requirement of undergraduate proficiency to graduate-level sophistication. Appendices provide an introduction to (or refresher on) some of the prerequisite material and exercises are integrated into the text, contributing to the volume’s ability to be used as a self-contained text. Readers will find the presentation especially useful for independent study or as a supplement to a graduate course in fixed-point theory. The material is split into four parts: the first introduces the Banach Contraction-Mapping Principle and the Brouwer Fixed-Point Theorem, along with a selection of interesting applications; the second focuses on Brouwer’s theorem and its application to John Nash’s work; the third applies Brouwer’s theorem to spaces of infinite dimension; and the fourth rests ...

  11. Zeta potentials of the rare earth element fluorcarbonate minerals focusing on bastnäsite and parisite.

    Science.gov (United States)

    Owens, C L; Nash, G R; Hadler, K; Fitzpatrick, R S; Anderson, C G; Wall, F

    2018-06-01

    Rare earth elements (REE) are critical to a wide range of technologies ranging from mobile phones to wind turbines. Processing and extraction of REE minerals from ore bodies is, however, both challenging and relatively poorly understood, as the majority of deposits contain only limited enrichment of REEs. An improved understanding of the surface properties of the minerals is important in informing and optimising their processing, in particular for separation by froth flotation. The measurement of zeta potential can be used to extract information regarding the electrical double layer, and hence surface properties of these minerals. There are over 34 REE fluorcarbonate minerals currently identified, however bastnäsite, synchysite and parisite are of most economic importance. Bastnäsite-(Ce), the most common REE fluorcarbonate, supplies over 50% of the world's REE. Previous studies of bastnäsite have showed a wide range of surface behaviour, with the iso-electric point (IEP), being measured between pH values of 4.6 and 9.3. In contrast, no values of IEP have been reported for parisite or synchysite. In this work, we review previous studies of the zeta potentials of bastnäsite to investigate the effects of different methodologies and sample preparation. In addition, measurements of zeta potentials of parisite under water, collector and supernatant conditions were conducted, the first to be reported. These results showed an iso-electric point for parisite of 5.6 under water, with a shift to a more negative zeta potential with both collector (hydroxamic and fatty acids) and supernatant conditions. The IEP with collectors and supernatant was <3.5. As zeta potential measurements in the presence of reagents and supernatants are the most rigorous way of determining the efficiency of a flotation reagent, the agreement between parisite zeta potentials obtained here and previous work on bastnäsite suggests that parisite may be processed using similar reagent schemes to

  12. Zero-point oscillations, zero-point fluctuations, and fluctuations of zero-point oscillations

    International Nuclear Information System (INIS)

    Khalili, Farit Ya

    2003-01-01

    Several physical effects and methodological issues relating to the ground state of an oscillator are considered. Even in the simplest case of an ideal lossless harmonic oscillator, its ground state exhibits properties that are unusual from the classical point of view. In particular, the mean value of the product of two non-negative observables, kinetic and potential energies, is negative in the ground state. It is shown that semiclassical and rigorous quantum approaches yield substantially different results for the ground state energy fluctuations of an oscillator with finite losses. The dependence of zero-point fluctuations on the boundary conditions is considered. Using this dependence, it is possible to transmit information without emitting electromagnetic quanta. Fluctuations of electromagnetic pressure of zero-point oscillations are analyzed, and the corresponding mechanical friction is considered. This friction can be viewed as the most fundamental mechanism limiting the quality factor of mechanical oscillators. Observation of these effects exceeds the possibilities of contemporary experimental physics but almost undoubtedly will be possible in the near future. (methodological notes)

  13. Point-point and point-line moving-window correlation spectroscopy and its applications

    Science.gov (United States)

    Zhou, Qun; Sun, Suqin; Zhan, Daqi; Yu, Zhiwu

    2008-07-01

    In this paper, we present a new extension of generalized two-dimensional (2D) correlation spectroscopy. Two new algorithms, namely point-point (P-P) correlation and point-line (P-L) correlation, have been introduced to do the moving-window 2D correlation (MW2D) analysis. The new method has been applied to a spectral model consisting of two different processes. The results indicate that P-P correlation spectroscopy can unveil the details and re-constitute the entire process, whilst the P-L can provide general feature of the concerned processes. Phase transition behavior of dimyristoylphosphotidylethanolamine (DMPE) has been studied using MW2D correlation spectroscopy. The newly proposed method verifies that the phase transition temperature is 56 °C, same as the result got from a differential scanning calorimeter. To illustrate the new method further, a lysine and lactose mixture has been studied under thermo perturbation. Using the P-P MW2D, the Maillard reaction of the mixture was clearly monitored, which has been very difficult using conventional display of FTIR spectra.

  14. The Point Lepreau Desktop Simulator

    International Nuclear Information System (INIS)

    MacLean, M.; Hogg, J.; Newman, H.

    1997-01-01

    The Point Lepreau Desktop Simulator runs plant process modeling software on a 266 MHz single CPU DEC Alpha computer. This same Alpha also runs the plant control computer software on an SSCI 125 emulator. An adjacent Pentium PC runs the simulator's Instructor Facility software, and communicates with the Alpha through an Ethernet. The Point Lepreau Desktop simulator is constructed to be as similar as possible to the Point Lepreau full scope training simulator. This minimizes total maintenance costs and enhances the benefits of the desktop simulator. Both simulators have the same modeling running on a single CPU in the same schedule of calculations. Both simulators have the same Instructor Facility capable of developing and executing the same lesson plans, doing the same monitoring and control of simulations, inserting all the same malfunctions, performing all the same overrides, capable of making and restoring all the same storepoints. Both simulators run the same plant control computer software - the same assembly language control programs as the power plant uses for reactor control, heat transport control, annunciation, etc. This is a higher degree of similarity between a desktop simulator and a full scope training simulator than previously reported for a computer controlled nuclear plant. The large quantity of control room hardware missing from the desktop simulator is replaced by software. The Instructor Facility panel override software of the training simulator provides the means by which devices (switches, controllers, windows, etc.) on the control room panels can be controlled and monitored in the desktop simulator. The CRT of the Alpha provides a mouse operated DCC keyboard mimic for controlling the plant control computer emulation. Two emulated RAMTEK display channels appear as windows for monitoring anything of interest on plant DCC displays, including one channel for annunciation. (author)

  15. Growth points in nuclear physics

    CERN Document Server

    Hodgson, Peter Edward

    1980-01-01

    Growth Points in Nuclear Physics, Volume 2 covers the progress in the fields of nuclear structure and nuclear reactions. This book is composed of three chapters. The first chapter is devoted to nuclear forces and potentials, in particular the optical model potential that enables the elastic scattering of many particles by nuclei to be calculated in a very simple manner. This chapter also deals with the three-body forces and the spin dependence of the nuclear potential. The second chapter describes higher order processes involving two or more stages, specifically their intrinsic interest and th

  16. Processing Terrain Point Cloud Data

    KAUST Repository

    DeVore, Ronald

    2013-01-10

    Terrain point cloud data are typically acquired through some form of Light Detection And Ranging sensing. They form a rich resource that is important in a variety of applications including navigation, line of sight, and terrain visualization. Processing terrain data has not received the attention of other forms of surface reconstruction or of image processing. The goal of terrain data processing is to convert the point cloud into a succinct representation system that is amenable to the various application demands. The present paper presents a platform for terrain processing built on the following principles: (i) measuring distortion in the Hausdorff metric, which we argue is a good match for the application demands, (ii) a multiscale representation based on tree approximation using local polynomial fitting. The basic elements held in the nodes of the tree can be efficiently encoded, transmitted, visualized, and utilized for the various target applications. Several challenges emerge because of the variable resolution of the data, missing data, occlusions, and noise. Techniques for identifying and handling these challenges are developed. © 2013 Society for Industrial and Applied Mathematics.

  17. Address Points - COUNTY_ADDRESS_POINTS_IDHS_IN: Address Points Maintained by County Agencies in Indiana (Indiana Department of Homeland Security, Point feature class)

    Data.gov (United States)

    NSGIC State | GIS Inventory — COUNTY_ADDRESS_POINTS_IDHS_IN is an ESRI Geodatabase point feature class that contains address points maintained by county agencies in Indiana, provided by personnel...

  18. Glacial Features (Point) - Quad 168 (EPPING, NH)

    Data.gov (United States)

    University of New Hampshire — The Glacial Features (Point) layer describes point features associated with surficial geology. These glacial features include, but are not limited to, delta forsets,...

  19. Possible refurbishment of Point Lepreau

    International Nuclear Information System (INIS)

    White, R.M.; Groom, S.H.; Thompson, P.D.; Barclay, J.M.; Allen, P.J.

    2001-01-01

    In February 2000, the NB Power Board of Directors approved Phase one of a project to produce a business case including a detailed scope and estimate associated with the possible refurbishment of the Point Lepreau Generating Station (PLGS). The Preliminary plan for refurbishment projects an 18-month outage starting as early as the spring of 2006. If the station were to be refurbished, then it would be run for another 25 to 30 years. The decision on whether or not to refurbish PLGS has not been made and is not expected until the summer of 2002. The results of the first phase of the project will be used to prepare a detailed business case that will be presented to the NB Power board of directors in January of 2002. At that time a decision will be made as to whether to refurbish the unit, or obtain other means of replacing the energy produced by PLGS. The station currently produces about a third of the power generated within the province. If the business case is approved, all-380 Pressure Tubes and Calandria Tubes, along with their related End Fittings and Feeders would be replaced. This material would be stored in new storage vaults to be constructed at the existing on-site Waste Management Facility. Replacement of other station components will be performed as required, as determined from the results of a comprehensive Plant Condition Assessment. The condition assessments build on work done under the Plant Life Management Program. Point Lepreau Generating Station has operated well since start of commercial operation in early 1983. With a lifetime capacity factor of about 84% (up to the end of 2000), it has proven to be an economic and environmentally sound electricity provider. The station has also had a significant positive economic impact in Southern New Brunswick, employing over 600 people. However the Pressure Tubes and Feeders are nearing the point in time in which they will exceed their fitness for service criteria. Although tubes can be replaced on an

  20. Possible refurbishment of Point Lepreau

    International Nuclear Information System (INIS)

    White, R.M.; Groom, S.H.; Thompson, P.D.; Barclay, J.M.; Allen, P.J.

    2001-01-01

    In February 2000, the NB Power Board of Directors approved Phase one of a project to produce a business case including a detailed scope and estimate associated with the possible refurbishment of the Point Lepreau Generating Station (PLGS). The Preliminary plan for refurbishment projects an 18-month outage starting as early as the spring of 2006. If the station were to be refurbished, then it would be run for another 25 to 30 years. The decision on whether or not to refurbish PLGS has not been made and is not expected until the summer of 2002. The results of the first phase of the project will be used to prepare a detailed business case that will be presented to the NB Power board of directors in January of 2002. At that time a decision will be made as to whether to refurbish the unit, or obtain other means of replacing the energy produced by PLGS. The station currently produces about a third of the power generated within the province. If the business case is approved, all-380 Pressure Tubes and Calandria Tubes, along with their related End Fittings and Feeders would be replaced. This material would be stored in new storage vaults to be constructed at the existing on-site Waste Management Facility. Replacement of other station components will be performed as required, as determined from the results of a comprehensive Plant Condition Assessment. The condition assessments build on work done under the Plant Life Management Program. Point Lepreau Generating Station has operated well since start of commercial operation in early 1983. With a lifetime capacity factor of about 84% (up to the end of 2000), it has proven to be an economic and environmentally sound electricity provider. The station has also had a significant positive economic impact in Southern New Brunswick, employing over 600 people. However the Pressure Tubes and Feeders are nearing the point in time in which they will exceed their fitness for service criteria. Although tubes can be replaced on an

  1. Rational points on elliptic curves

    CERN Document Server

    Silverman, Joseph H

    2015-01-01

    The theory of elliptic curves involves a pleasing blend of algebra, geometry, analysis, and number theory. This book stresses this interplay as it develops the basic theory, thereby providing an opportunity for advanced undergraduates to appreciate the unity of modern mathematics. At the same time, every effort has been made to use only methods and results commonly included in the undergraduate curriculum. This accessibility, the informal writing style, and a wealth of exercises make Rational Points on Elliptic Curves an ideal introduction for students at all levels who are interested in learning about Diophantine equations and arithmetic geometry. Most concretely, an elliptic curve is the set of zeroes of a cubic polynomial in two variables. If the polynomial has rational coefficients, then one can ask for a description of those zeroes whose coordinates are either integers or rational numbers. It is this number theoretic question that is the main subject of this book. Topics covered include the geometry and ...

  2. The physics of exceptional points

    International Nuclear Information System (INIS)

    Heiss, W D

    2012-01-01

    A short résumé is given about the nature of exceptional points (EPs) followed by discussions about their ubiquitous occurrence in a great variety of physical problems. EPs feature in classical as well as in quantum mechanical problems. They are associated with symmetry breaking for PT-symmetric Hamiltonians, where a great number of experiments has been performed, in particular in optics, and to an increasing extent in atomic and molecular physics. EPs are involved in quantum phase transition and quantum chaos; they produce dramatic effects in multichannel scattering, specific time dependence and more. In nuclear physics, they are associated with instabilities and continuum problems. Being spectral singularities they also affect approximation schemes. This article is part of a special issue of Journal of Physics A: Mathematical and Theoretical devoted to ‘Quantum physics with non-Hermitian operators’. (paper)

  3. Photovoltaics at Point Pelee Park

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1996-12-01

    Case study of an Ontario Hydro-installed photovoltaic system at Point Pelee Park, a bird sanctuary located on Lake Erie, is described. The system consists of a 1080 W photovoltaic array used to supply electricity to one of the washrooms. The cost for installing the system was $30,000 which was considerably cheaper than the $100,000 estimate for an underground power line. The independent system is the only source of energy for the washroom, therefore it was necessary to reduce the total electrical demand required by the facility. Electricity was used for the water pump, chlorinator and lighting. Motion sensors were installed to further reduce electrical demand. Washroom heaters were converted to propane. 2 figs.

  4. Preferred design of transfer points

    Energy Technology Data Exchange (ETDEWEB)

    Firstbrook, J

    1983-06-01

    The paper reports the results of a study carried out at MRDE on the performance of transfer points. These sites still require a considerable deployment of manpower. The investigations were divided into 3 main sections: mineral handling; chute design; belt cleaning and spillage conveying. In order to achieve a sufficient level of reliability of a transfer system, it is considered that the coal size should not be greater than 250 mm. Different designs of gravity transfer chute were studied and 3 basic designs emerged as being effective : dump chute, curved site entry, and asymmetric side entry. Selection is dependent on conveyor sizes, speeds, and mineral throughput. Individual belt cleaners are not sufficient to clean belts carrying typical run-of-mine material: two cleaners followed by a set of squeeze rollers are recommended. Several designs of spillage conveyor were investigated; at the present time the electro/hydraulic type appears to be the most satisfactory.

  5. Point-source inversion techniques

    Science.gov (United States)

    Langston, Charles A.; Barker, Jeffrey S.; Pavlin, Gregory B.

    1982-11-01

    A variety of approaches for obtaining source parameters from waveform data using moment-tensor or dislocation point source models have been investigated and applied to long-period body and surface waves from several earthquakes. Generalized inversion techniques have been applied to data for long-period teleseismic body waves to obtain the orientation, time function and depth of the 1978 Thessaloniki, Greece, event, of the 1971 San Fernando event, and of several events associated with the 1963 induced seismicity sequence at Kariba, Africa. The generalized inversion technique and a systematic grid testing technique have also been used to place meaningful constraints on mechanisms determined from very sparse data sets; a single station with high-quality three-component waveform data is often sufficient to discriminate faulting type (e.g., strike-slip, etc.). Sparse data sets for several recent California earthquakes, for a small regional event associated with the Koyna, India, reservoir, and for several events at the Kariba reservoir have been investigated in this way. Although linearized inversion techniques using the moment-tensor model are often robust, even for sparse data sets, there are instances where the simplifying assumption of a single point source is inadequate to model the data successfully. Numerical experiments utilizing synthetic data and actual data for the 1971 San Fernando earthquake graphically demonstrate that severe problems may be encountered if source finiteness effects are ignored. These techniques are generally applicable to on-line processing of high-quality digital data, but source complexity and inadequacy of the assumed Green's functions are major problems which are yet to be fully addressed.

  6. Point to point multispectral light projection applied to cultural heritage

    Science.gov (United States)

    Vázquez, D.; Alvarez, A.; Canabal, H.; Garcia, A.; Mayorga, S.; Muro, C.; Galan, T.

    2017-09-01

    Use of new of light sources based on LED technology should allow the develop of systems that combine conservation and exhibition requirements and allow to make these art goods available to the next generations according to sustainability principles. The goal of this work is to develop light systems and sources with an optimized spectral distribution for each specific point of the art piece. This optimization process implies to maximize the color fidelity reproduction and the same time to minimize the photochemical damage. Perceived color under these sources will be similar (metameric) to technical requirements given by the restoration team uncharged of the conservation and exhibition of the goods of art. Depending of the fragility of the exposed art objects (i.e. spectral responsivity of the material) the irradiance must be kept under a critical level. Therefore, it is necessary to develop a mathematical model that simulates with enough accuracy both the visual effect of the illumination and the photochemical impact of the radiation. Spectral reflectance of a reference painting The mathematical model is based on a merit function that optimized the individual intensity of the LED-light sources taking into account the damage function of the material and color space coordinates. Moreover the algorithm used weights for damage and color fidelity in order to adapt the model to a specific museal application. In this work we show a sample of this technology applied to a picture of Sorolla (1863-1923) an important Spanish painter title "woman walking at the beach".

  7. Acid dew point measurement in flue gases

    Energy Technology Data Exchange (ETDEWEB)

    Struschka, M.; Baumbach, G.

    1986-06-01

    The operation of modern boiler plants requires the continuous measurement of the acid dew point in flue gases. An existing measuring instrument was modified in such a way that it can determine acid dew points reliably, reproduceably and continuously. The authors present the mechanisms of the dew point formation, the dew point measuring principle, the modification and the operational results.

  8. 18 CFR 157.211 - Delivery points.

    Science.gov (United States)

    2010-04-01

    ... 18 Conservation of Power and Water Resources 1 2010-04-01 2010-04-01 false Delivery points. 157... for Certain Transactions and Abandonment § 157.211 Delivery points. (a) Construction and operation—(1... delivery point, excluding the construction of certain delivery points subject to the prior notice...

  9. Professional SharePoint 2010 Development

    CERN Document Server

    Rizzo, Tom; Fried, Jeff

    2010-01-01

    Learn to leverage the features of the newest version of SharePoint, in this update to the bestseller. More than simply a portal, SharePoint is Microsoft's popular content management solution for building intranets and Web sites or hosting wikis and blogs. Offering broad coverage on all aspects of development for the SharePoint platform, this comprehensive book shows you exactly what SharePoint does, how to build solutions, and what features are accessible within SharePoint. Written by one of the most recognized names in SharePoint development, Professional SharePoint 2010 Development offers an

  10. MOVING WINDOW SEGMENTATION FRAMEWORK FOR POINT CLOUDS

    Directory of Open Access Journals (Sweden)

    G. Sithole

    2012-07-01

    Full Text Available As lidar point clouds become larger streamed processing becomes more attractive. This paper presents a framework for the streamed segmentation of point clouds with the intention of segmenting unstructured point clouds in real-time. The framework is composed of two main components. The first component segments points within a window shifting over the point cloud. The second component stitches the segments within the windows together. In this fashion a point cloud can be streamed through these two components in sequence, thus producing a segmentation. The algorithm has been tested on airborne lidar point cloud and some results of the performance of the framework are presented.

  11. Yield point of metallic glass

    International Nuclear Information System (INIS)

    Shimizu, Futoshi; Ogata, Shigenobu; Li, Ju

    2006-01-01

    Shear bands form in most bulk metallic glasses (BMGs) within a narrow range of uniaxial strain ε y ≅ 2%. We propose this critical condition corresponds to embryonic shear band (ESB) propagation, not its nucleation. To propagate an ESB, the far-field shear stress τ ∞ ∼ Eε y /2 must exceed the quasi-steady-state glue traction τ glue of shear-alienated glass until the glass transition temperature is approached internally due to frictional heating, at which point ESB matures as a runaway shear crack. The incubation length scale l inc necessary for this maturation is estimated to be ∼10 2 nm for Zr-based BMGs, below which sample size-scale shear localization does not happen. In shear-alienated glass, the last resistance against localized shearing comes from extremely fast downhill dissipative dynamics of timescale comparable to atomic vibrations, allowing molecular dynamics (MD) simulations to capture this recovery process which governs τ glue . We model four metallic glasses: a binary Lennard-Jones system, two binary embedded atom potential systems and a quinternary embedded atom system. Despite vast differences in the structure and interatomic interactions, the four MD calculations give ε y predictions of 2.4%, 2.1%, 2.6% and 2.9%, respectively

  12. Anesthesiology Point of Care project.

    Science.gov (United States)

    McDonald, John S; Noback, Carl R; Cheng, Drew; Lee, T K; Nenov, Val

    2002-01-01

    We are developing a dynamic prototype visual communication system for the operating room environs. This has classically been viewed as an isolated and impenetrable workplace. All medical experiences and all teaching remain in a one to one closed loop with no recall or subsequent sharing for the training and education of other colleagues. The "Anesthesia Point of Care" (APOC) concept embraces the sharing of, recording of, and presentation of various physiological and pharmacological events so that real time memory can be shared at a later time for the edification of other colleagues who were not present at the time of the primary learning event. In addition it also provides a remarkably rapid tool for fellow faculty to respond to obvious stress and crisis events that can be broadcast instantly at the time of happening. Finally, it also serves as an efficient and effective means of paging and general communication throughout the daily routines among various healthcare providers in anesthesiology who work as a team unit; these include the staff, residents, CRNAs, physician assistants, and technicians. This system offers a unique opportunity to eventually develop future advanced ideas that can include training exercises, presurgical evaluations, surgical scheduling and improvements in efficiency based upon earlier than expected case completion or conversely later than expected case completion and even as a unique window to development of improved billing itemization and coordination.

  13. Decision Point 1 Topical Report

    Energy Technology Data Exchange (ETDEWEB)

    Yablonsky, Al; Barsoumian, Shant; Legere, David

    2013-05-01

    This Topical Report addresses accomplishments achieved during Phase 2a of the SkyMine® Carbon Mineralization Pilot Project. The primary objectives of this project are to design, construct, and operate a system to capture CO2 from a slipstream of flue gas from a commercial coal-fired cement kiln, convert that CO2 to products having commercial value (i.e., beneficial use), show the economic viability of the CO2 capture and conversion process, and thereby advance the technology to the point of readiness for commercial scale demonstration and proliferation. The overall process is carbon negative, resulting in mineralization of CO2 that would otherwise be released into the atmosphere. The project will also substantiate market opportunities for the technology by sales of chemicals into existing markets, and identify opportunities to improve technology performance and reduce costs at the commercial scale. The project is being conducted in two phases. The primary objectives of Phase 1 were to elaborate proven SkyMine® process chemistry to commercial pilot-scale operation and complete the preliminary design for the pilot plant to be built and operated in Phase 2, complete a NEPA evaluation, and develop a comprehensive carbon life cycle analysis. The objective of the current Phase (2a) is to complete the detailed design of the pilot plant to be built in Phase 2b.

  14. Gravity and Zero Point Energy

    Science.gov (United States)

    Massie, U. W.

    When Planck introduced the 1/2 hv term to his 1911 black body equation he showed that there is a residual energy remaining at zero degree K after all thermal energy ceased. Other investigators, including Lamb, Casimir, and Dirac added to this information. Today zero point energy (ZPE) is accepted as an established condition. The purpose of this paper is to demonstrate that the density of the ZPE is given by the gravity constant (G) and the characteristics of its particles are revealed by the cosmic microwave background (CMB). Eddies of ZPE particles created by flow around mass bodies reduce the pressure normal to the eddy flow and are responsible for the force of gravity. Helium atoms resonate with ZPE particles at low temperature to produce superfluid helium. High velocity micro vortices of ZPE particles about a basic particle or particles are responsible for electromagnetic forces. The speed of light is the speed of the wave front in the ZPE and its value is a function of the temperature and density of the ZPE.

  15. Tipping Points, Great and Small

    Science.gov (United States)

    Morrison, Foster

    2010-12-01

    The Forum by Jordan et al. [2010] addressed environmental problems of various scales in great detail, but getting the critical message through to the formulators of public policies requires going back to basics, namely, that exponential growth (of a population, an economy, or most anything else) is not sustainable. When have you heard any politician or economist from anywhere across the ideological spectrum say anything other than that more growth is essential? There is no need for computer models to demonstrate “limits to growth,” as was done in the 1960s. Of course, as one seeks more details, the complexity of modeling will rapidly outstrip the capabilities of both observation and computing. This is common with nonlinear systems, even simple ones. Thus, identifying all possible “tipping points,” as suggested by Jordan et al. [2010], and then stopping just short of them, is impractical if not impossible. The main thing needed to avoid environmental disasters is a bit of common sense.

  16. Turkey Point tritium. Progress report

    International Nuclear Information System (INIS)

    Ostlund, H.G.; Dorsey, H.G.

    1976-01-01

    In 1972-73 the Florida Power and Light Company (FPL) began operation of two nuclear reactors at Turkey Point on lower Biscayne Bay. One radioactive by-product resulting from the operation of the nuclear reactors, tritium, provides a unique opportunity to study transport and exchange processes on a local scale. Since the isotope in the form of water is not removed from the liquid effluent, it is discharged to the cooling canal system. By studying its residence time in the canal and the pathways by which it leaves the canals, knowledge of evaporative process, groundwater movement, and bay exchange with the ocean can be obtained. Preliminary results obtained from measurement of tritium levels, both in the canal system and in the surrounding environment are discussed. Waters in lower Biscayne Bay and Card and Barnes Sounds receive only a small portion of the total tritium produced by the nuclear plant. The dominating tritium loss most likely is through evaporation from the canals. The capability of measuring extremely low HTO levels allows the determination of the evaporation rate experimentally by measuring the tritium levels of air after having passed over the canals

  17. Beginning SharePoint 2010 Administration Windows SharePoint Foundation 2010 and Microsoft SharePoint Server 2010

    CERN Document Server

    Husman, Göran

    2010-01-01

    Complete coverage on the latest advances in SharePoint 2010 administration. SharePoint 2010 comprises an abundance of new features, and this book shows you how to take advantage of all SharePoint 2010's many improvements. Written by a four-time SharePoint MVP, Beginning SharePoint 2010 Administration begins with a comparison of SharePoint 2010 compared to the previous version and then examines the differences between WSS 4.0 and MSS 2010. Packed with step-by-step instructions, tips and tricks, and real-world examples, this book dives into the basics of how to install, manage, and administrate

  18. Comparison of Dose When Prescribed to Point A and Point H for Brachytherapy in Cervical Cancer

    Energy Technology Data Exchange (ETDEWEB)

    Gang, Ji Hyeong; Gim, Il Hwan; Hwang, Seon Boong; Kim, Woong; Im, Hyeong Seo; Gang, Jin Mook; Gim, Gi Hwan; Lee, Ah Ram [Dept. of Radiation Oncology, Korea Institute of Radiological and Medical Sciences, Seou (Korea, Republic of)

    2012-09-15

    The purpose of this study is to compare plans prescribed to point A with these prescribed to point H recommended by ABS (American Brachytherapy Society) in high dose rate intracavitary brachytherapy for cervical carcinoma. This study selected 103 patients who received HDR (High Dose Rate) brachytherapy using tandem and ovoids from March 2010 to January 2012. Point A, bladder point, and rectal point conform with Manchester System. Point H conforms with ABS recommendation. Also Sigmoid colon point, and vagina point were established arbitrarily. We examined distance between point A and point H. The percent dose at point A was calculated when 100% dose was prescribed to point H. Additionally, the percent dose at each reference points when dose is prescribed to point H and point A were calculated. The relative dose at point A was lower when point H was located inferior to point A. The relative doses at bladder, rectal, sigmoid colon, and vagina points were higher when point H was located superior to point A, and lower when point H was located inferior to point A. This study found out that as point H got located much superior to point A, the absorbed dose of surrounding normal organs became higher, and as point H got located much inferior to point A, the absorbed dose of surrounding normal organs became lower. This differences dose not seem to affect the treatment. However, we suggest this new point is worth being considered for the treatment of HDR if dose distribution and absorbed dose at normal organs have large differences between prescribed to point A and H.

  19. Point Lepreau refurbishment - update 5

    International Nuclear Information System (INIS)

    White, R.M.; Eagles, E.R.; Hickman, C.N.; Baker, R.; Thompson, P.D.; Howieson, J.Q.; Ichiyen, N.

    2005-01-01

    NB Power Nuclear is planning to conduct a 18-month maintenance outage of the Point Lepreau Generating Station (PLGS) beginning in April 2008. The major activity would be the replacement of all 380 Fuel Channel and Calandria Tube assemblies and connecting feeder pipes. This activity is referred to as Retube. NB Power Nuclear would also take advantage of this outage to conduct a number of repairs, replacements, inspection and upgrades (such as rewinding or replacing the generator, replacement of shutdown system trip computers, replacement of certain valves and expansion joints, inspection of systems not normally accessible, etc.). These collective activities are referred to as Refurbishment. This would allow the station to operate for an additional 25 to 30 years. The scope or the project was determined [mm the outcome of a two year study involving a detailed condition assessment of the station which examined Issues relating to ageing and obsolescence, along with a detailed review or Safety and Licensing issues associated with extended operation. The Refurbishment outage would be preceded by a detailed Engineering Project Phase that would: Finalize details of the Retube process including modeling, tooling development, site facilities and training of personnel. Perform necessary engineering activities related to design modifications. Construct the new waste storage structures to house Retube Waste and other additional waste storage structures for the extended life of the station. Setup necessary temporary construction facilities (offices, storage areas, change moms, decontamination an maintenance areas) to support Retube. Procure equipment and components. Perform detailed outage planning. Initiate development of detailed commissioning as well as lay-up, monitoring and return to service procedures. At the present time, the NB Power Nuclear Board of Directors and the New Brunswick Provincial government are reviewing a proposal for a lease arrangement from Bruce Power

  20. Professional SharePoint 2010 Administration

    CERN Document Server

    Klindt, Todd; Caravajal, Steve

    2010-01-01

    Thorough coverage of the improvements and changes to SharePoint 2010. SharePoint 2010 boasts a variety of incredible new features that will challenge even the most experienced administrator who is upgrading from SharePoint 2007. Written by a team of SharePoint experts, this book places a takes aim at showing you how to make these new features work right for you. Offering an in-depth look at SharePoint 2010, the authors focus on how SharePoint functionality has changed from its earliest version to its newest, and they provide you with detailed coverage of all the new features and capabilities.:

  1. SharePoint 2010 Field Guide

    CERN Document Server

    Mann, Steven; Gazmuri, Pablo; Caravajal, Steve; Wheeler, Christina

    2012-01-01

    Hands-on solutions for common SharePoint 2010 challenges Aimed at the more than 100 million licensed SharePoint 2010 users, this indispensable field guide addresses an abundance of common SharePoint 2010 problems and offers proven solutions. A team of authors encourages you to customize SharePoint beyond the out-of-the-box functionality so that you can build more complex solutions to these challenges. You?ll discover intricate details and specific full-scale solutions that you can then implement to your own SharePoint 2010 solutions.Tackles a variety of SharePoint 2010 problems ranging from si

  2. Professional SharePoint 2010 Development

    CERN Document Server

    Rizzo, Tom; Fried, Jeff; Swider, Paul J; Hillier, Scot; Schaefer, Kenneth

    2012-01-01

    Updated guidance on how to take advantage of the newest features of SharePoint programmability More than simply a portal, SharePoint is Microsoft's popular content management solution for building intranets and websites or hosting wikis and blogs. Offering broad coverage on all aspects of development for the SharePoint platform, this comprehensive book shows you exactly what SharePoint does, how to build solutions, and what features are accessible within SharePoint. Written by a team of SharePoint experts, this new edition offers an extensive selection of field-tested best practices that shows

  3. Professional SharePoint 2013 administration

    CERN Document Server

    Young, Shane; Klindt, Todd

    2013-01-01

    SharePoint admin author gurus return to prepare you for working with the new features of SharePoint 2013! The new iteration of SharePoint boasts exciting new features. However, any new version also comes with its fair share of challenges and that's where this book comes in. The team of SharePoint admin gurus returns to presents a fully updated resource that prepares you for making all the new SharePoint 2013 features work right. They cover all of the administration components of SharePoint 2013 in detail, and present a clear understanding of how they affect the role of the adminis

  4. SharePoint 2010 For Dummies

    CERN Document Server

    Williams, Vanessa L

    2012-01-01

    Here's the bestselling guide on SharePoint 2010, updated to cover Office 365 SharePoint Portal Server is an essential part of the enterprise infrastructure for many businesses. The Office 365 version includes significantly enhanced cloud capabilities. This second edition of the bestselling guide to SharePoint covers getting a SharePoint site up and running, branded, populated with content, and more. It explains ongoing site management and offers plenty of advice for administrators who want to leverage SharePoint and Office 365 in various ways.Many businesses today rely on SharePoint Portal Ser

  5. Testing Local Independence between Two Point Processes

    DEFF Research Database (Denmark)

    Allard, Denis; Brix, Anders; Chadæuf, Joël

    2001-01-01

    Independence test, Inhomogeneous point processes, Local test, Monte Carlo, Nonstationary, Rotations, Spatial pattern, Tiger bush......Independence test, Inhomogeneous point processes, Local test, Monte Carlo, Nonstationary, Rotations, Spatial pattern, Tiger bush...

  6. State Wildlife Management Area Public Facilities - points

    Data.gov (United States)

    Minnesota Department of Natural Resources — This point theme contains facilities and features for WMAs that are best represented as points. WMAs are part of the Minnesota state recreation system created to...

  7. Implementation of Steiner point of fuzzy set.

    Science.gov (United States)

    Liang, Jiuzhen; Wang, Dejiang

    2014-01-01

    This paper deals with the implementation of Steiner point of fuzzy set. Some definitions and properties of Steiner point are investigated and extended to fuzzy set. This paper focuses on establishing efficient methods to compute Steiner point of fuzzy set. Two strategies of computing Steiner point of fuzzy set are proposed. One is called linear combination of Steiner points computed by a series of crisp α-cut sets of the fuzzy set. The other is an approximate method, which is trying to find the optimal α-cut set approaching the fuzzy set. Stability analysis of Steiner point of fuzzy set is also studied. Some experiments on image processing are given, in which the two methods are applied for implementing Steiner point of fuzzy image, and both strategies show their own advantages in computing Steiner point of fuzzy set.

  8. The Science of Drug Use: Discussion Points

    Science.gov (United States)

    ... The Science of Drug Use: Discussion Points The Science of Drug Use: Discussion Points Email Facebook Twitter ... was last updated February 2017 Related Topics Addiction Science Adolescent Brain Comorbidity College-Age & Young Adults Criminal ...

  9. Zero Point Energy of Renormalized Wilson Loops

    OpenAIRE

    Hidaka, Yoshimasa; Pisarski, Robert D.

    2009-01-01

    The quark antiquark potential, and its associated zero point energy, can be extracted from lattice measurements of the Wilson loop. We discuss a unique prescription to renormalize the Wilson loop, for which the perturbative contribution to the zero point energy vanishes identically. A zero point energy can arise nonperturbatively, which we illustrate by considering effective string models. The nonperturbative contribution to the zero point energy vanishes in the Nambu model, but is nonzero wh...

  10. Residual analysis for spatial point processes

    DEFF Research Database (Denmark)

    Baddeley, A.; Turner, R.; Møller, Jesper

    We define residuals for point process models fitted to spatial point pattern data, and propose diagnostic plots based on these residuals. The techniques apply to any Gibbs point process model, which may exhibit spatial heterogeneity, interpoint interaction and dependence on spatial covariates. Ou...... or covariate effects. Q-Q plots of the residuals are effective in diagnosing interpoint interaction. Some existing ad hoc statistics of point patterns (quadrat counts, scan statistic, kernel smoothed intensity, Berman's diagnostic) are recovered as special cases....

  11. Influence of the burning point on the dew point in a diesel engine

    Energy Technology Data Exchange (ETDEWEB)

    Teetz, C.

    1982-06-01

    A computation on the influence of the ignition point on the dew point in a cylinder of a diesel engine is presented. The cylinder-pressure diagrams are shown. The results of computation are given. A later ignition point diminishes the area with cylinder wall temperatures below the dew point. The endangering by cylinder wall temperatures below the dew point is illustrated.

  12. Towards extending IFC with point cloud data

    NARCIS (Netherlands)

    Krijnen, T.F.; Beetz, J.; Ochmann, S.; Vock, R.; Wessel, R.

    2015-01-01

    In this paper we suggest an extension to the Industry Foundation Classes model to integrate point cloud datasets. The proposal includes a schema extension to the core model allowing the storage of points either as Cartesian coordinates, points in parametric space of a surface associated with a

  13. Moving window segmentation framework for point clouds

    NARCIS (Netherlands)

    Sithole, G.; Gorte, B.G.H.

    2012-01-01

    As lidar point clouds become larger streamed processing becomes more attractive. This paper presents a framework for the streamed segmentation of point clouds with the intention of segmenting unstructured point clouds in real-time. The framework is composed of two main components. The first

  14. Computing bubble-points of CO

    NARCIS (Netherlands)

    Ramdin, M.; Balaji, S.P.; Vicent Luna, J.M.; Torres-Knoop, A; Chen, Q.; Dubbeldam, D.; Calero, S; de Loos, T.W.; Vlugt, T.J.H.

    2016-01-01

    Computing bubble-points of multicomponent mixtures using Monte Carlo simulations is a non-trivial task. A new method is used to compute gas compositions from a known temperature, bubble-point pressure, and liquid composition. Monte Carlo simulations are used to calculate the bubble-points of

  15. Inhomogeneous Markov point processes by transformation

    DEFF Research Database (Denmark)

    Jensen, Eva B. Vedel; Nielsen, Linda Stougaard

    2000-01-01

    We construct parametrized models for point processes, allowing for both inhomogeneity and interaction. The inhomogeneity is obtained by applying parametrized transformations to homogeneous Markov point processes. An interesting model class, which can be constructed by this transformation approach......, is that of exponential inhomogeneous Markov point processes. Statistical inference For such processes is discussed in some detail....

  16. RELATIONSHIP BETWEEN FLASH POINTS OF SOME BINARY ...

    African Journals Online (AJOL)

    B. S. Chandravanshi

    Miscellaneous binary blends containing solvent neutral-150 (SN-150), ... viscosity, the flash point test has always been a standard part of a lubricant's specification. ... between structure and flash points of organic compounds [5-12] and fuels [13, 14]. ... in binary mixtures, the gaps between flash points would be high enough.

  17. How PowerPoint Is Killing Education

    Science.gov (United States)

    Isseks, Marc

    2011-01-01

    Although it is essential to incorporate new technologies into the classroom, says Isseks, one trend has negatively affected instruction--the misuse of PowerPoint presentations. The author describes how poorly designed PowerPoint presentations reduce complex thoughts to bullet points and reduce the act of learning to transferring text from slide to…

  18. Characterization of finite spaces having dispersion points

    International Nuclear Information System (INIS)

    Al-Bsoul, A. T

    1997-01-01

    In this paper we shall characterize the finite spaces having dispersion points. Also, we prove that the dispersion point of a finite space with a dispersion points fixed under all non constant continuous functions which answers the question raised by J. C obb and W. Voxman in 1980 affirmatively for finite space. Some open problems are given. (author). 16 refs

  19. The Fermat point for a taxicab triangle

    Science.gov (United States)

    Hanson, J. R.

    2015-04-01

    The Fermat point P for a triangle ABC is the point P the sum of whose distances from the vertices A, B and C is a minimum. This note will show how to find the Fermat point for any triangle using the taxicab metric.

  20. SharePoint 2013 for dummies

    CERN Document Server

    Withee, Ken

    2013-01-01

    The bestselling guide on running SharePoint, now updated to cover all the new features of SharePoint 2013 SharePoint Portal Server is an essential part of the enterprise infrastructure for many businesses. Building on the success of previous versions of SharePoint For Dummies, this new edition covers all the latest features of SharePoint 2013 and provides you with an easy-to-understand resource for making the most of all that this version has to offer. You'll learn how to get a site up and running, branded, and populated with content, workflow, and management. In addition, t

  1. Pro SharePoint 2013 administration

    CERN Document Server

    Garrett, Robert

    2013-01-01

    Pro SharePoint 2013 Administration is a practical guide to SharePoint 2013 for intermediate to advanced SharePoint administrators and power users, covering the out-of-the-box feature set and capabilities of Microsoft's collaboration and business productivity platform. SharePoint 2013 is an incredibly complex product, with many moving parts, new features, best practices, and 'gotchas.' Author Rob Garrett distills SharePoint's portfolio of features, capabilities, and utilities into an in-depth professional guide-with no fluff and copious advice-that is designed from scratch to be the manual Micr

  2. Equipartitioning and balancing points of polygons

    Directory of Open Access Journals (Sweden)

    Shunmugam Pillay

    2010-07-01

    Full Text Available The centre of mass G of a triangle has the property that the rays to the vertices from G sweep out triangles having equal areas. We show that such points, termed equipartitioning points in this paper, need not exist in other polygons. A necessary and sufficient condition for a quadrilateral to have an equipartitioning point is that one of its diagonals bisects the other. The general theorem, namely, necessary and sufficient conditions for equipartitioning points for arbitrary polygons to exist, is also stated and proved. When this happens, they are in general, distinct from the centre of mass. In parallelograms, and only in them, do the two points coincide.

  3. Beginning SharePoint Designer 2010

    CERN Document Server

    Windischman, Woodrow W; Rehmani, Asif

    2010-01-01

    Teaching Web designers, developers, and IT professionals how to use the new version of SharePoint Designer. Covering both the design and business applications of SharePoint Designer, this complete Wrox guide brings readers thoroughly up to speed on how to use SharePoint Designer in an enterprise. You'll learn to create and modify web pages, use CSS editing tools to modify themes, use Data View to create interactivity with SharePoint and other data, and much more. Coverage includes integration points with Visual Studio, Visio, and InfoPath.: Shows web designers, developers, and IT professionals

  4. Zero point energy of renormalized Wilson loops

    International Nuclear Information System (INIS)

    Hidaka, Yoshimasa; Pisarski, Robert D.

    2009-01-01

    The quark-antiquark potential, and its associated zero point energy, can be extracted from lattice measurements of the Wilson loop. We discuss a unique prescription to renormalize the Wilson loop, for which the perturbative contribution to the zero point energy vanishes identically. A zero point energy can arise nonperturbatively, which we illustrate by considering effective string models. The nonperturbative contribution to the zero point energy vanishes in the Nambu model, but is nonzero when terms for extrinsic curvature are included. At one loop order, the nonperturbative contribution to the zero point energy is negative, regardless of the sign of the extrinsic curvature term.

  5. Pro SharePoint 2010 Search

    CERN Document Server

    Noble, J; Bakman-Mikalski, Dan

    2011-01-01

    Pro SharePoint 2010 Search gives you expert advice on planning, deploying and customizing searches in SharePoint 2010. Drawing on the authors' extensive experience of working with real-world SharePoint deployments, this book teaches everything you'll need to know to create well-designed SharePoint solutions that always keep the end-user's experience in mind. Increase your search efficiency with SharePoint 2010's search functionality: extend the search user interface using third-party tools, and utilize analytics to improve relevancy. This practical hands-on book is a must-have resource for any

  6. X-point effect on edge stability

    International Nuclear Information System (INIS)

    Saarelma, S; Kirk, A; Kwon, O J

    2011-01-01

    We study the effects of the X-point configuration on edge localized mode (ELM) triggering peeling and ballooning modes using fixed boundary equilibria and modifying the plasma shape to approach the limit of a true X-point. The current driven pure peeling modes are asymptotically stabilized by the X-point while the stabilizing effect on ballooning modes depends on the poloidal location of the X-point. The coupled peeling-ballooning modes experience the elimination of the peeling component as the X-point is introduced. This can significantly affect the edge stability diagrams used to analyse the ELM triggering mechanisms.

  7. Critical point analysis of phase envelope diagram

    Energy Technology Data Exchange (ETDEWEB)

    Soetikno, Darmadi; Siagian, Ucok W. R. [Department of Petroleum Engineering, Institut Teknologi Bandung, Jl. Ganesha 10, Bandung 40132 (Indonesia); Kusdiantara, Rudy, E-mail: rkusdiantara@s.itb.ac.id; Puspita, Dila, E-mail: rkusdiantara@s.itb.ac.id; Sidarto, Kuntjoro A., E-mail: rkusdiantara@s.itb.ac.id; Soewono, Edy; Gunawan, Agus Y. [Department of Mathematics, Institut Teknologi Bandung, Jl. Ganesha 10, Bandung 40132 (Indonesia)

    2014-03-24

    Phase diagram or phase envelope is a relation between temperature and pressure that shows the condition of equilibria between the different phases of chemical compounds, mixture of compounds, and solutions. Phase diagram is an important issue in chemical thermodynamics and hydrocarbon reservoir. It is very useful for process simulation, hydrocarbon reactor design, and petroleum engineering studies. It is constructed from the bubble line, dew line, and critical point. Bubble line and dew line are composed of bubble points and dew points, respectively. Bubble point is the first point at which the gas is formed when a liquid is heated. Meanwhile, dew point is the first point where the liquid is formed when the gas is cooled. Critical point is the point where all of the properties of gases and liquids are equal, such as temperature, pressure, amount of substance, and others. Critical point is very useful in fuel processing and dissolution of certain chemicals. Here in this paper, we will show the critical point analytically. Then, it will be compared with numerical calculations of Peng-Robinson equation by using Newton-Raphson method. As case studies, several hydrocarbon mixtures are simulated using by Matlab.

  8. Critical point analysis of phase envelope diagram

    International Nuclear Information System (INIS)

    Soetikno, Darmadi; Siagian, Ucok W. R.; Kusdiantara, Rudy; Puspita, Dila; Sidarto, Kuntjoro A.; Soewono, Edy; Gunawan, Agus Y.

    2014-01-01

    Phase diagram or phase envelope is a relation between temperature and pressure that shows the condition of equilibria between the different phases of chemical compounds, mixture of compounds, and solutions. Phase diagram is an important issue in chemical thermodynamics and hydrocarbon reservoir. It is very useful for process simulation, hydrocarbon reactor design, and petroleum engineering studies. It is constructed from the bubble line, dew line, and critical point. Bubble line and dew line are composed of bubble points and dew points, respectively. Bubble point is the first point at which the gas is formed when a liquid is heated. Meanwhile, dew point is the first point where the liquid is formed when the gas is cooled. Critical point is the point where all of the properties of gases and liquids are equal, such as temperature, pressure, amount of substance, and others. Critical point is very useful in fuel processing and dissolution of certain chemicals. Here in this paper, we will show the critical point analytically. Then, it will be compared with numerical calculations of Peng-Robinson equation by using Newton-Raphson method. As case studies, several hydrocarbon mixtures are simulated using by Matlab

  9. Handbook of floating-point arithmetic

    CERN Document Server

    Muller, Jean-Michel; de Dinechin, Florent; Jeannerod, Claude-Pierre; Joldes, Mioara; Lefèvre, Vincent; Melquiond, Guillaume; Revol, Nathalie; Torres, Serge

    2018-01-01

    This handbook is a definitive guide to the effective use of modern floating-point arithmetic, which has considerably evolved, from the frequently inconsistent floating-point number systems of early computing to the recent IEEE 754-2008 standard. Most of computational mathematics depends on floating-point numbers, and understanding their various implementations will allow readers to develop programs specifically tailored for the standard’s technical features. Algorithms for floating-point arithmetic are presented throughout the book and illustrated where possible by example programs which show how these techniques appear in actual coding and design. The volume itself breaks its core topic into four parts: the basic concepts and history of floating-point arithmetic; methods of analyzing floating-point algorithms and optimizing them; implementations of IEEE 754-2008 in hardware and software; and useful extensions to the standard floating-point system, such as interval arithmetic, double- and triple-word arithm...

  10. Interval Mathematics Applied to Critical Point Transitions

    Directory of Open Access Journals (Sweden)

    Benito A. Stradi

    2012-03-01

    Full Text Available The determination of critical points of mixtures is important for both practical and theoretical reasons in the modeling of phase behavior, especially at high pressure. The equations that describe the behavior of complex mixtures near critical points are highly nonlinear and with multiplicity of solutions to the critical point equations. Interval arithmetic can be used to reliably locate all the critical points of a given mixture. The method also verifies the nonexistence of a critical point if a mixture of a given composition does not have one. This study uses an interval Newton/Generalized Bisection algorithm that provides a mathematical and computational guarantee that all mixture critical points are located. The technique is illustrated using several example problems. These problems involve cubic equation of state models; however, the technique is general purpose and can be applied in connection with other nonlinear problems.

  11. SharePoint 2007 Collaboration For Dummies

    CERN Document Server

    Harvey, Greg

    2009-01-01

    If you're looking for a way to help your teams access what they need to know, work together, and get the job done, SharePoint can do just that. SharePoint 2007 Collaboration For Dummies shows you the easiest way to set up and customize SharePoint, manage your data, interact using SharePoint blogs and wikis, integrate Office programs, and make your office more productive. You'll learn what SharePoint can do and how to make it work for your business, understand the technical terms, and enable your people to collaborate on documents and spreadsheets. You'll even discover how to get SharePoint hel

  12. Describing chaotic attractors: Regular and perpetual points

    Science.gov (United States)

    Dudkowski, Dawid; Prasad, Awadhesh; Kapitaniak, Tomasz

    2018-03-01

    We study the concepts of regular and perpetual points for describing the behavior of chaotic attractors in dynamical systems. The idea of these points, which have been recently introduced to theoretical investigations, is thoroughly discussed and extended into new types of models. We analyze the correlation between regular and perpetual points, as well as their relation with phase space, showing the potential usefulness of both types of points in the qualitative description of co-existing states. The ability of perpetual points in finding attractors is indicated, along with its potential cause. The location of chaotic trajectories and sets of considered points is investigated and the study on the stability of systems is shown. The statistical analysis of the observing desired states is performed. We focus on various types of dynamical systems, i.e., chaotic flows with self-excited and hidden attractors, forced mechanical models, and semiconductor superlattices, exhibiting the universality of appearance of the observed patterns and relations.

  13. Algorithms for solving common fixed point problems

    CERN Document Server

    Zaslavski, Alexander J

    2018-01-01

    This book details approximate solutions to common fixed point problems and convex feasibility problems in the presence of perturbations. Convex feasibility problems search for a common point of a finite collection of subsets in a Hilbert space; common fixed point problems pursue a common fixed point of a finite collection of self-mappings in a Hilbert space. A variety of algorithms are considered in this book for solving both types of problems, the study of which has fueled a rapidly growing area of research. This monograph is timely and highlights the numerous applications to engineering, computed tomography, and radiation therapy planning. Totaling eight chapters, this book begins with an introduction to foundational material and moves on to examine iterative methods in metric spaces. The dynamic string-averaging methods for common fixed point problems in normed space are analyzed in Chapter 3. Dynamic string methods, for common fixed point problems in a metric space are introduced and discussed in Chapter ...

  14. Kantian Turning Point in Gadamer's Philosophical Hermeneutics

    Directory of Open Access Journals (Sweden)

    Kristína Bosáková

    2016-11-01

    Full Text Available The paper is treating the theme of a Kantian turning-point in the philosophical hermeneutics of H.- G. Gadamer based on of the harmonic relationship between metaphysics and science in Kantian philosophy from the point of view of the philosophical hermeneutics of Gadamer. The philosophical work of Kant had such an influence on Gadamer that without exaggerating we can talk about the Kantian turning-point in Gadamerian hermeneutics. Grondin, a former student of Gadamer, is talking about Kantian turning-point on the field of aesthetics, but in reality Kantian turning-point means much more than a mere change in the reception of the concept of judgement. It is a discovery of harmonical relationship between the beauty and the moral, between the reason and the sensitivity, between the modern sciences and the metaphysical tradition in the Kantian philosophy, made by Gadamer. This is what we call the Kantian turning-point in Gadamerian hermeneutics.

  15. Fixed points of occasionally weakly biased mappings

    OpenAIRE

    Y. Mahendra Singh, M. R. Singh

    2012-01-01

    Common fixed point results due to Pant et al. [Pant et al., Weak reciprocal continuity and fixed point theorems, Ann Univ Ferrara, 57(1), 181-190 (2011)] are extended to a class of non commuting operators called occasionally weakly biased pair[ N. Hussain, M. A. Khamsi A. Latif, Commonfixed points for JH-operators and occasionally weakly biased pairs under relaxed conditions, Nonlinear Analysis, 74, 2133-2140 (2011)]. We also provideillustrative examples to justify the improvements. Abstract....

  16. Common fixed points for weakly compatible maps

    Indian Academy of Sciences (India)

    Springer Verlag Heidelberg #4 2048 1996 Dec 15 10:16:45

    In 1976, Jungck [4] proved a common fixed point theorem for commuting maps generalizing the Banach's fixed point theorem, which states that, 'let (X, d) be a complete metric space. If T satisfies d(Tx,Ty) ≤ kd(x,y) for each x,y ∈ X where 0 ≤ k < 1, then T has a unique fixed point in X'. This theorem has many applications, ...

  17. Infra-red fixed points in supersymmetry

    Indian Academy of Sciences (India)

    ¾c /font>, and c stands for the color quadratic Casimir of the field. Fixed points arise when R* ¼ or when R*. /nobr>. ´S-½. µ ´r ·b¿µ. The stability of the solutions may be tested by linearizing the system about the fixed points. For the non-trivial fixed points we need to consider the eigenvalues of the stability matrix whose ...

  18. Bayesian analysis of Markov point processes

    DEFF Research Database (Denmark)

    Berthelsen, Kasper Klitgaard; Møller, Jesper

    2006-01-01

    Recently Møller, Pettitt, Berthelsen and Reeves introduced a new MCMC methodology for drawing samples from a posterior distribution when the likelihood function is only specified up to a normalising constant. We illustrate the method in the setting of Bayesian inference for Markov point processes...... a partially ordered Markov point process as the auxiliary variable. As the method requires simulation from the "unknown" likelihood, perfect simulation algorithms for spatial point processes become useful....

  19. Professional SharePoint 2013 development

    CERN Document Server

    Alirezaei, Reza; Ranlett, Matt; Hillier, Scot; Wilson, Brian; Fried, Jeff; Swider, Paul

    2013-01-01

    Thorough coverage of development in SharePoint 2013 A team of well-known Microsoft MVPs joins forces in this fully updated resource, providing you with in-depth coverage of development tools in the latest iteration of the immensely popular SharePoint. From building solutions to building custom workflow and content management applications, this book shares field-tested best practices on all aspect of SharePoint 2013 development. Offers a thorough look at Windows Azure and SharePoint 2013Includes new chapters on Application Life Cycle Management, developing apps in ShareP

  20. Calculation of melting points of oxides

    International Nuclear Information System (INIS)

    Bobkova, O.S.; Voskobojnikov, V.G.; Kozin, A.I.

    1975-01-01

    The correlation between the melting point and thermodynamic parameters characterizing the strength of oxides and compounds is given. Such thermodynamic paramters include the energy and antropy of atomization

  1. Beginning SharePoint 2010 Development

    CERN Document Server

    Fox, Steve

    2010-01-01

    Discover how to take advantage of the many new features in SharePoint 2010. SharePoint provides content management (enterprise content management, Web content management, records management, and more), workflow, and social media features, and the new version boasts enhanced capabilities. This introductory-level book walks you through the process of learning, developing, and deploying SharePoint 2010 solutions. You'll leverage your existing skills and tools to grasp the fundamental programming concepts and practices of SharePoint 2010. The author clearly explains how to develop your first appli

  2. EPA Region 1 Environmentally Sensitive Areas (Points)

    Data.gov (United States)

    U.S. Environmental Protection Agency — This coverage represents point equivalents of environmentally sensitive areas in EPA New England. This coverage represents polygon equivalents of environmentally...

  3. PowerPoint 2007 for Dummies

    CERN Document Server

    Lowe, Doug

    2007-01-01

    New and inexperienced PowerPoint users will discover how to use the latest enhancements to PowerPoint 2007 quickly and efficiently so that they can produce unique and informative presentations PowerPoint continues to be the world's most popular presentation software This updated For Dummies guide shows users different ways to create powerful and effective slideshow presentations that incorporate data from other applications in the form of charts, clip art, sound, and video Shares the key features of PowerPoint 2007 including creating and editing slides, working with hyperlinks and action butt

  4. Blossom Point Satellite Tracking and Command Station

    Data.gov (United States)

    Federal Laboratory Consortium — FUNCTION: The Blossom Point Satellite Command and Tracking Facility (BP) provides engineering and operational support to several complex space systems for the Navy...

  5. Track length estimation applied to point detectors

    International Nuclear Information System (INIS)

    Rief, H.; Dubi, A.; Elperin, T.

    1984-01-01

    The concept of the track length estimator is applied to the uncollided point flux estimator (UCF) leading to a new algorithm of calculating fluxes at a point. It consists essentially of a line integral of the UCF, and although its variance is unbounded, the convergence rate is that of a bounded variance estimator. In certain applications, involving detector points in the vicinity of collimated beam sources, it has a lower variance than the once-more-collided point flux estimator, and its application is more straightforward

  6. Mastering Microsoft SharePoint Foundation 2010

    CERN Document Server

    Callahan, C A

    2011-01-01

    Everything IT professionals need to create collaborative solutions. SharePoint Foundation 2010 is the newest version of a powerful collaboration tool used in many Exchange-enabled organizations. This book gets network professionals and business application administrators up to speed on the updates, features, and installation procedures, preparing them to create powerful collaboration structures for their companies.: Microsoft SharePoint Foundation 2010 is the successor to Windows SharePoint Services 3.0 and is used with Microsoft Office SharePoint Server to enable collaboration; this guide pre

  7. Teach yourself visually PowerPoint 2013

    CERN Document Server

    Wood, William

    2013-01-01

    A straightforward, visual approach to learning the new PowerPoint 2013! PowerPoint 2013 boasts updated features and new possibilities; this highly visual tutorial provides step-by-step instructions to help you learn all the capabilities of PowerPoint 2013. It covers the basics, as well as all the exciting new changes and additions in a series of easy-to-follow, full-color, two-page tutorials. Learn how to create slides, dress them up using templates and graphics, add sound and animation, and more. This book is the ideal ""show me, don't tell me"" guide to PowerPoint 2013.De

  8. Wolf Point Substation, Roosevelt County, Montana

    International Nuclear Information System (INIS)

    1991-05-01

    The Western Area Power Administration (Western), an agency of the United States Department of Energy, is proposing to construct the 115-kV Wolf Point Substation near Wolf Point in Roosevelt County, Montana (Figure 1). As part of the construction project, Western's existing Wolf Point Substation would be taken out of service. The existing 115-kV Wolf Point Substation is located approximately 3 miles west of Wolf Point, Montana (Figure 2). The substation was constructed in 1949. The existing Wolf Point Substation serves as a ''Switching Station'' for the 115-kV transmission in the region. The need for substation improvements is based on operational and reliability issues. For this environmental assessment (EA), the environmental review of the proposed project took into account the removal of the old Wolf Point Substation, rerouting of the five Western lines and four lines from the Cooperatives and Montana-Dakota Utilities Company, and the new road into the proposed substation. Reference to the new proposed Wolf Point Substation in the EA includes these facilities as well as the old substation site. The environmental review looked at the impacts to all resource areas in the Wolf Point area. 7 refs., 6 figs

  9. Parametric Architectural Design with Point-clouds

    DEFF Research Database (Denmark)

    Zwierzycki, Mateusz; Evers, Henrik Leander; Tamke, Martin

    2016-01-01

    This paper investigates the efforts and benefits of the implementation of point clouds into architectural design processes and tools. Based on a study on the principal work processes of designers with point clouds the prototypical plugin/library - Volvox - was developed for the parametric modelling...

  10. Point cloud data management (extended abstract)

    NARCIS (Netherlands)

    Van Oosterom, P.J.M.; Ravada, S.; Horhammer, M.; Martinez Rubi, O.; Ivanova, M.; Kodde, M.; Tijssen, T.P.M.

    2014-01-01

    Point cloud data are important sources for 3D geo-information. The point cloud data sets are growing in popularity and in size. Modern Big Data acquisition and processing technologies, such as laser scanning from airborne, mobile, or static platforms, dense image matching from photos, multi-beam

  11. The Psychic Organ Point of Autistic Syntax

    Science.gov (United States)

    Amir, Dana

    2013-01-01

    This paper deals with autistic syntax and its expressions both in the fully fledged autistic structure and in the autistic zones of other personality structures. The musical notion of the organ point serves as a point of departure in an attempt to describe how autistic syntax transforms what was meant to constitute the substrate for linguistic…

  12. Finite Elements on Point Based Surfaces

    NARCIS (Netherlands)

    Clarenz, U.; Rumpf, M.; Telea, A.

    2004-01-01

    We present a framework for processing point-based surfaces via partial differential equations (PDEs). Our framework efficiently and effectively brings well-known PDE-based processing techniques to the field of point-based surfaces. Our method is based on the construction of local tangent planes and

  13. Magnetic Fixed Points and Emergent Supersymmetry

    DEFF Research Database (Denmark)

    Antipin, Oleg; Mojaza, Matin; Pica, Claudio

    2013-01-01

    We establish in perturbation theory the existence of fixed points along the renormalization group flow for QCD with an adjoint Weyl fermion and scalar matter reminiscent of magnetic duals of QCD [1-3]. We classify the fixed points by analyzing their basin of attraction. We discover that among...

  14. The improbability of Harris interest points

    DEFF Research Database (Denmark)

    Loog, Marco; Lauze, Francois Bernard

    2010-01-01

    An elementary characterization of the map underlying Harris corners, also known as Harris interest points or key points, is provided. Two principal and basic assumptions made are: 1) Local image structure is captured in an uncommitted way, simply using weighted raw image values around every image...

  15. Gravitational Zero Point Energy induces Physical Observables

    OpenAIRE

    Garattini, Remo

    2010-01-01

    We consider the contribution of Zero Point Energy on the induced Cosmological Constant and on the induced Electric/Magnetic charge in absence of matter fields. The method is applicable to every spherically symmetric background. Extensions to a generic $f(R) $ theory are also allowed. Only the graviton appears to be fundamental to the determination of Zero Point Energy.

  16. Zero-point energy in spheroidal geometries

    OpenAIRE

    Kitson, A. R.; Signal, A. I.

    2005-01-01

    We study the zero-point energy of a massless scalar field subject to spheroidal boundary conditions. Using the zeta-function method, the zero-point energy is evaluated for small ellipticity. Axially symmetric vector fields are also considered. The results are interpreted within the context of QCD flux tubes and the MIT bag model.

  17. State estimation for temporal point processes

    NARCIS (Netherlands)

    van Lieshout, Maria Nicolette Margaretha

    2015-01-01

    This paper is concerned with combined inference for point processes on the real line observed in a broken interval. For such processes, the classic history-based approach cannot be used. Instead, we adapt tools from sequential spatial point processes. For a range of models, the marginal and

  18. Metallic and antiferromagnetic fixed points from gravity

    Science.gov (United States)

    Paul, Chandrima

    2018-06-01

    We consider SU(2) × U(1) gauge theory coupled to matter field in adjoints and study RG group flow. We constructed Callan-Symanzik equation and subsequent β functions and study the fixed points. We find there are two fixed points, showing metallic and antiferromagnetic behavior. We have shown that metallic phase develops an instability if certain parametric conditions are satisfied.

  19. Developing a Teachable Point of View

    Science.gov (United States)

    Cameron, Kim

    2007-01-01

    As professors of management and organizational behavior mature in their teaching, they should begin to develop a teachable point of view. In this article, the author describes several attributes of a teachable point of view. Based on his own teaching experience, the author outlines five criteria for the content of the material taught--the what of…

  20. The Improbability of Harris Interest Points

    NARCIS (Netherlands)

    Loog, M.; Lauze, F.B.

    2010-01-01

    An elementary characterization of the map underlying Harris corners, also known as Harris interest points or key points, is provided. Two principal and basic assumptions made are: 1) Local image structure is captured in an uncommitted way, simply using weighted raw image values around every image

  1. Impedance analysis of acupuncture points and pathways

    International Nuclear Information System (INIS)

    Teplan, Michal; Kukucka, Marek; Ondrejkovicová, Alena

    2011-01-01

    Investigation of impedance characteristics of acupuncture points from acoustic to radio frequency range is addressed. Discernment and localization of acupuncture points in initial single subject study was unsuccessfully attempted by impedance map technique. Vector impedance analyses determined possible resonant zones in MHz region.

  2. TOPOLOGY OF RANDOM POINTS YOGESHWARAN. D.

    Indian Academy of Sciences (India)

    Balls grow at unit rate centred at the points of the point cloud/ process. ... Idea of persistence : Keep track of births and deaths of topological features. ..... holes, Betti numbers, etc., one will be more interested in the distribution of such objects on ...

  3. Fixed points in a group of isometries

    NARCIS (Netherlands)

    Voorneveld, M.

    2000-01-01

    The Bruhat-Tits xed point theorem states that a group of isometries on a complete metric space with negative curvature possesses a xed point if it has a bounded orbit. This theorem is extended by a relaxation of the negative curvature condition in terms of the w-distance functions introduced by Kada

  4. Determinants of College Grade Point Averages

    Science.gov (United States)

    Bailey, Paul Dean

    2012-01-01

    Chapter 2: The Role of Class Difficulty in College Grade Point Averages. Grade Point Averages (GPAs) are widely used as a measure of college students' ability. Low GPAs can remove a students from eligibility for scholarships, and even continued enrollment at a university. However, GPAs are determined not only by student ability but also by the…

  5. NIM Realization of the Gallium Triple Point

    Science.gov (United States)

    Xiaoke, Yan; Ping, Qiu; Yuning, Duan; Yongmei, Qu

    2003-09-01

    In the last three years (1999 to 2001), the gallium triple-point cell has been successfully developed, and much corresponding research has been carried out at the National Institute of Metrology (NIM), Beijing, China. This paper presents the cell design, apparatus and procedure for realizing the gallium triple point, and presents studies on the different freezing methods. The reproducibility is 0.03 mK, and the expanded uncertainty of realization of the gallium triple point is evaluated to be 0.17 mK (p=0.99, k=2.9). Also, the reproducibility of the gallium triple point was compared with that of the triple point of water.

  6. MODEL FOR SEMANTICALLY RICH POINT CLOUD DATA

    Directory of Open Access Journals (Sweden)

    F. Poux

    2017-10-01

    Full Text Available This paper proposes an interoperable model for managing high dimensional point clouds while integrating semantics. Point clouds from sensors are a direct source of information physically describing a 3D state of the recorded environment. As such, they are an exhaustive representation of the real world at every scale: 3D reality-based spatial data. Their generation is increasingly fast but processing routines and data models lack of knowledge to reason from information extraction rather than interpretation. The enhanced smart point cloud developed model allows to bring intelligence to point clouds via 3 connected meta-models while linking available knowledge and classification procedures that permits semantic injection. Interoperability drives the model adaptation to potentially many applications through specialized domain ontologies. A first prototype is implemented in Python and PostgreSQL database and allows to combine semantic and spatial concepts for basic hybrid queries on different point clouds.

  7. Model for Semantically Rich Point Cloud Data

    Science.gov (United States)

    Poux, F.; Neuville, R.; Hallot, P.; Billen, R.

    2017-10-01

    This paper proposes an interoperable model for managing high dimensional point clouds while integrating semantics. Point clouds from sensors are a direct source of information physically describing a 3D state of the recorded environment. As such, they are an exhaustive representation of the real world at every scale: 3D reality-based spatial data. Their generation is increasingly fast but processing routines and data models lack of knowledge to reason from information extraction rather than interpretation. The enhanced smart point cloud developed model allows to bring intelligence to point clouds via 3 connected meta-models while linking available knowledge and classification procedures that permits semantic injection. Interoperability drives the model adaptation to potentially many applications through specialized domain ontologies. A first prototype is implemented in Python and PostgreSQL database and allows to combine semantic and spatial concepts for basic hybrid queries on different point clouds.

  8. Thinning spatial point processes into Poisson processes

    DEFF Research Database (Denmark)

    Møller, Jesper; Schoenberg, Frederic Paik

    2010-01-01

    are identified, and where we simulate backwards and forwards in order to obtain the thinned process. In the case of a Cox process, a simple independent thinning technique is proposed. In both cases, the thinning results in a Poisson process if and only if the true Papangelou conditional intensity is used, and......In this paper we describe methods for randomly thinning certain classes of spatial point processes. In the case of a Markov point process, the proposed method involves a dependent thinning of a spatial birth-and-death process, where clans of ancestors associated with the original points......, thus, can be used as a graphical exploratory tool for inspecting the goodness-of-fit of a spatial point process model. Several examples, including clustered and inhibitive point processes, are considered....

  9. Thinning spatial point processes into Poisson processes

    DEFF Research Database (Denmark)

    Møller, Jesper; Schoenberg, Frederic Paik

    , and where one simulates backwards and forwards in order to obtain the thinned process. In the case of a Cox process, a simple independent thinning technique is proposed. In both cases, the thinning results in a Poisson process if and only if the true Papangelou conditional intensity is used, and thus can......This paper describes methods for randomly thinning certain classes of spatial point processes. In the case of a Markov point process, the proposed method involves a dependent thinning of a spatial birth-and-death process, where clans of ancestors associated with the original points are identified...... be used as a diagnostic for assessing the goodness-of-fit of a spatial point process model. Several examples, including clustered and inhibitive point processes, are considered....

  10. SharePoint 2010 Development For Dummies

    CERN Document Server

    Withee, Ken

    2011-01-01

    A much-needed guide that shows you how to leverage SharePoint tools without writing a line of code!. The great news about SharePoint is…you don't need to be a seasoned .NET developer to develop custom functions for it. This book shows you how to use SharePoint Designer, Report Builder, Dashboard Designer, InfoPath, Excel, Word, Visio, and the SharePoint web-based UI to design and develop—without ever writing a line of code! Learn how to customize your site, build SharePoint apps, start social networking, or add Web parts.  This straightforward guide makes everything easier.: Introduces you to

  11. Wrox SharePoint 2010 SharePoint911 three-pack

    CERN Document Server

    Klindt, Todd; Mason, Jennifer; Rogers, Laura; Drisgill, Randy; Ross, John; Riemann, Larry; Perran, Amanda; Perran, Shane; Sanford, Jacob J; Stubbs, Paul; Caravajal, Steve

    2012-01-01

    The Wrox SharePoint 2010 SharePoint911 Three-Pack combines the contents of three full e-books written by the experts from SharePoint911.  That's over 1800 pages of hands-on advice from Todd Klindt, Shane Young, Laura Rogers, Randy Drisgill, Jennifer Mason, John Ross, and Larry Riemann, among others. In Beginning SharePoint 2010: Building Business Solutions with SharePoint (ISBN 978-0-470-61789-2) by Amanda Perran, Shane Perran, Jennifer Mason, and Laura Rogers, readers learn the core concepts, terminology, and features of SharePoint 2010. In Professiona

  12. Beginning SharePoint 2010 Building Business Solutions with SharePoint

    CERN Document Server

    Perran, Amanda; Mason, Jennifer; Rogers, Laura

    2010-01-01

    Two SharePoint MVPs provide the ultimate introduction to SharePoint 2010Beginning SharePoint 2010: Building Team Solutions with SharePoint provides information workers and site managers with extensive knowledge and expert advice, empowering them to become SharePoint champions within their organizations.Provides expansive coverage of SharePoint topics, as well as specialty areas such as forms, excel services, records management, and web content managementDetails realistic usage scenarios, and includes practice examples that highlight best practices for configuration and customizationIncludes de

  13. The power of PowerPoint.

    Science.gov (United States)

    Niamtu , J

    2001-08-01

    Carousel slide presentations have been used for academic and clinical presentations since the late 1950s. However, advances in computer technology have caused a paradigm shift, and digital presentations are quickly becoming standard for clinical presentations. The advantages of digital presentations include cost savings; portability; easy updating capability; Internet access; multimedia functions, such as animation, pictures, video, and sound; and customization to augment audience interest and attention. Microsoft PowerPoint has emerged as the most popular digital presentation software and is currently used by many practitioners with and without significant computer expertise. The user-friendly platform of PowerPoint enables even the novice presenter to incorporate digital presentations into his or her profession. PowerPoint offers many advanced options that, with a minimal investment of time, can be used to create more interactive and professional presentations for lectures, patient education, and marketing. Examples of advanced PowerPoint applications are presented in a stepwise manner to unveil the full power of PowerPoint. By incorporating these techniques, medical practitioners can easily personalize, customize, and enhance their PowerPoint presentations. Complications, pitfalls, and caveats are discussed to detour and prevent misadventures in digital presentations. Relevant Web sites are listed to further update, customize, and communicate PowerPoint techniques.

  14. Detecting Change-Point via Saddlepoint Approximations

    Institute of Scientific and Technical Information of China (English)

    Zhaoyuan LI; Maozai TIAN

    2017-01-01

    It's well-known that change-point problem is an important part of model statistical analysis.Most of the existing methods are not robust to criteria of the evaluation of change-point problem.In this article,we consider "mean-shift" problem in change-point studies.A quantile test of single quantile is proposed based on saddlepoint approximation method.In order to utilize the information at different quantile of the sequence,we further construct a "composite quantile test" to calculate the probability of every location of the sequence to be a change-point.The location of change-point can be pinpointed rather than estimated within a interval.The proposed tests make no assumptions about the functional forms of the sequence distribution and work sensitively on both large and small size samples,the case of change-point in the tails,and multiple change-points situation.The good performances of the tests are confirmed by simulations and real data analysis.The saddlepoint approximation based distribution of the test statistic that is developed in the paper is of independent interest and appealing.This finding may be of independent interest to the readers in this research area.

  15. Interest point detection for hyperspectral imagery

    Science.gov (United States)

    Dorado-Muñoz, Leidy P.; Vélez-Reyes, Miguel; Roysam, Badrinath; Mukherjee, Amit

    2009-05-01

    This paper presents an algorithm for automated extraction of interest points (IPs)in multispectral and hyperspectral images. Interest points are features of the image that capture information from its neighbours and they are distinctive and stable under transformations such as translation and rotation. Interest-point operators for monochromatic images were proposed more than a decade ago and have since been studied extensively. IPs have been applied to diverse problems in computer vision, including image matching, recognition, registration, 3D reconstruction, change detection, and content-based image retrieval. Interest points are helpful in data reduction, and reduce the computational burden of various algorithms (like registration, object detection, 3D reconstruction etc) by replacing an exhaustive search over the entire image domain by a probe into a concise set of highly informative points. An interest operator seeks out points in an image that are structurally distinct, invariant to imaging conditions, stable under geometric transformation, and interpretable which are good candidates for interest points. Our approach extends ideas from Lowe's keypoint operator that uses local extrema of Difference of Gaussian (DoG) operator at multiple scales to detect interest point in gray level images. The proposed approach extends Lowe's method by direct conversion of scalar operations such as scale-space generation, and extreme point detection into operations that take the vector nature of the image into consideration. Experimental results with RGB and hyperspectral images which demonstrate the potential of the method for this application and the potential improvements of a fully vectorial approach over band-by-band approaches described in the literature.

  16. Point Information Gain and Multidimensional Data Analysis

    Directory of Open Access Journals (Sweden)

    Renata Rychtáriková

    2016-10-01

    Full Text Available We generalize the point information gain (PIG and derived quantities, i.e., point information gain entropy (PIE and point information gain entropy density (PIED, for the case of the Rényi entropy and simulate the behavior of PIG for typical distributions. We also use these methods for the analysis of multidimensional datasets. We demonstrate the main properties of PIE/PIED spectra for the real data with the examples of several images and discuss further possible utilizations in other fields of data processing.

  17. Dynamics and mission design near libration points

    CERN Document Server

    Gómez, G; Llibre, J; Martínez, R

    2001-01-01

    It is well known that the restricted three-body problem has triangular equilibrium points. These points are linearly stable for values of the mass parameter, µ , below Routh's critical value, µ 1 . It is also known that in the spatial case they are nonlinearly stable, not for all the initial conditions in a neighborhood of the equilibrium points L 4 , L 5 but for a set of relatively large measures. This follows from the celebrated Kolmogorov-Arnold-Moser theorem. In fact there are neighborhoods of computable size for which one obtains "practical stability" in the sense that the massless partic

  18. PowerPoint 2013 for dummies

    CERN Document Server

    Lowe, Doug

    2013-01-01

    Get up and running with this full-color guide to PowerPoint 2013! PowerPoint, the number one presentation software, has been revised and improved with the introduction of Microsoft Office 2013. With this all-new, full-color book by your side, you will learn how to take full advantage of all of PowerPoint's powerful and dynamic capabilities. Bestselling veteran For Dummies, author Doug Lowe breaks it all down so that you can create a powerful and effective slideshow presentation with the new wide-screen theme and variant that incorporates videos, pictures, and shapes, and all

  19. The Antitriangular Factorization of Saddle Point Matrices

    KAUST Repository

    Pestana, J.

    2014-01-01

    Mastronardi and Van Dooren [SIAM J. Matrix Anal. Appl., 34 (2013), pp. 173-196] recently introduced the block antitriangular ("Batman") decomposition for symmetric indefinite matrices. Here we show the simplification of this factorization for saddle point matrices and demonstrate how it represents the common nullspace method. We show that rank-1 updates to the saddle point matrix can be easily incorporated into the factorization and give bounds on the eigenvalues of matrices important in saddle point theory. We show the relation of this factorization to constraint preconditioning and how it transforms but preserves the structure of block diagonal and block triangular preconditioners. © 2014 Society for Industrial and Applied Mathematics.

  20. The universal cardinal ordering of fixed points

    International Nuclear Information System (INIS)

    San Martin, Jesus; Moscoso, Ma Jose; Gonzalez Gomez, A.

    2009-01-01

    We present the theorem which determines, by a permutation, the cardinal ordering of fixed points for any orbit of a period doubling cascade. The inverse permutation generates the orbit and the symbolic sequence of the orbit is obtained as a corollary. Interestingly enough, it is important to point that this theorem needs no previous information about any other orbit; also the cardinal ordering is achieved automatically with no need to compare numerical values associated with every point of the orbit (as would be the case if kneading theory were used).