WorldWideScience

Sample records for investigating bacterial sources

  1. Investigating Primary Source Literacy

    Science.gov (United States)

    Archer, Joanne; Hanlon, Ann M.; Levine, Jennie A.

    2009-01-01

    Primary source research requires students to acquire specialized research skills. This paper presents results from a user study testing the effectiveness of a Web guide designed to convey the concepts behind "primary source literacy". The study also evaluated students' strengths and weaknesses when conducting primary source research. (Contains 3…

  2. Investigating scintillometer source areas

    Science.gov (United States)

    Perelet, A. O.; Ward, H. C.; Pardyjak, E.

    2017-12-01

    Scintillometry is an indirect ground-based method for measuring line-averaged surface heat and moisture fluxes on length scales of 0.5 - 10 km. These length scales are relevant to urban and other complex areas where setting up traditional instrumentation like eddy covariance is logistically difficult. In order to take full advantage of scintillometry, a better understanding of the flux source area is needed. The source area for a scintillometer is typically calculated as a convolution of point sources along the path. A weighting function is then applied along the path to compensate for a total signal contribution that is biased towards the center of the beam path, and decreasing near the beam ends. While this method of calculating the source area provides an estimate of the contribution of the total flux along the beam, there are still questions regarding the physical meaning of the weighted source area. These questions are addressed using data from an idealized experiment near the Salt Lake City International Airport in northern Utah, U.S.A. The site is a flat agricultural area consisting of two different land uses. This simple heterogeneity in the land use facilitates hypothesis testing related to source areas. Measurements were made with a two wavelength scintillometer system spanning 740 m along with three standard open-path infrared gas analyzer-based eddy-covariance stations along the beam path. This configuration allows for direct observations of fluxes along the beam and comparisons to the scintillometer average. The scintillometer system employed measures the refractive index structure parameter of air for two wavelengths of electromagnetic radiation, 880 μm and 1.86 cm to simultaneously estimate path-averaged heat and moisture fluxes, respectively. Meteorological structure parameters (CT2, Cq2, and CTq) as well as surface fluxes are compared for various amounts of source area overlap between eddy covariance and scintillometry. Additionally, surface

  3. Wautersia: The Contingency Water Container Bacterial Contamination Investigation

    Science.gov (United States)

    Shkedi, Brienne; Labuda, Laura; Bruce, Rebekah

    2009-01-01

    The Orbiter delivers water to the International Space Station (ISS) in Contingency Water Containers (CWCs) on each flight to the ISS. These CWCs are routinely sampled during each mission to verify the quality of the delivered water. Of the 5 samples returned on STS118/ 13A.1 in August 2007, two exhibited microbial growth exceeding potable water acceptability limits and historical data by orders of magnitude . The microbe was identified as Wautersia species and an investigation was launched to find the source of the contamination. Since then, samples collected on subsequent flights indicated additional CWCs had the same bacteria, as well as several on-orbit systems. An investigation was launched to try to find and address the source of the bacterial contamination. This paper will discuss how Wautersia was found, what Wautersia is, the investigation, and resolution.

  4. Investigation on Ion Source Parameters

    CERN Document Server

    M. Cheikh Mhamed, S. Essabaa, C. Lau

    The EURISOL multi-mega-watt target station requires dedicated radioactive ion sources. Notably, they must be capable of operating under extremely hard radiations and with a larger fission target producing over 1014 fissions/s. The realisation of next-generation ion sources suitable for such operating conditions needs exhaustive studies and developments. In order to take up such a challenge, a review on radioactive ion sources was achieved and the investigation on ion source parameters was in particular focused on a plasma ion source through a R&D program.

  5. Elevator buttons as unrecognized sources of bacterial colonization in hospitals.

    Science.gov (United States)

    Kandel, Christopher E; Simor, Andrew E; Redelmeier, Donald A

    2014-01-01

    Elevators are ubiquitous and active inside hospitals, potentially facilitating bacterial transmission. The objective of this study was to estimate the prevalence of bacterial colonization on elevator buttons in large urban teaching hospitals. A total of 120 elevator buttons and 96 toilet surfaces were swabbed over separate intervals at 3 tertiary care hospitals on weekdays and weekends in Toronto, Ontario. For the elevators, swabs were taken from 2 interior buttons (buttons for the ground floor and one randomly selected upper-level floor) and 2 exterior buttons (the "up" button from the ground floor and the "down" button from the upper-level floor). For the toilet surfaces, swabs were taken from the exterior and interior handles of the entry door, the privacy latch, and the toilet flusher. Samples were obtained using standard bacterial collection techniques, followed by plating, culture, and species identification by a technician blind to sample source. The prevalence of colonization of elevator buttons was 61% (95% confidence interval 52%-70%). No significant differences in colonization prevalence were apparent in relation to location of the buttons, day of the week, or panel position within the elevator. Coagulase-negative staphylococci were the most common organisms cultured, whereas Enterococcus and Pseudomonas species were infrequent. Elevator buttons had a higher prevalence of colonization than toilet surfaces (61% v. 43%, p = 0.008). Hospital elevator buttons were commonly colonized by bacteria, although most pathogens were not clinically relevant. The risk of pathogen transmission might be reduced by simple countermeasures.

  6. A CASE STUDY OF NONPOINT SOURCES BACTERIAL CONTRIBUTION TO RURAL SURFACE WATER

    Science.gov (United States)

    The presentation will address several bacterial issues affecting the Turkey Creek (TC) watershed, in north central Ok. Our results from seasonal stream Escherichia coli (E. coli) analysis, bacterial source tracking, and antibiotic resistance will be shared and discussed in relat...

  7. Kisameet Glacial Clay: an Unexpected Source of Bacterial Diversity.

    Science.gov (United States)

    Svensson, Sarah L; Behroozian, Shekooh; Xu, Wanjing; Surette, Michael G; Li, Loretta; Davies, Julian

    2017-05-23

    Widespread antibiotic resistance among bacterial pathogens is providing the impetus to explore novel sources of antimicrobial agents. Recently, the potent antibacterial activity of certain clay minerals has stimulated scientific interest in these materials. One such example is Kisameet glacial clay (KC), an antibacterial clay from a deposit on the central coast of British Columbia, Canada. However, our understanding of the active principles of these complex natural substances is incomplete. Like soils, clays may possess complex mixtures of bacterial taxa, including the Actinobacteria , a clade known to be rich in antibiotic-producing organisms. Here, we present the first characterization of both the microbial and geochemical characteristics of a glacial clay deposit. KC harbors surprising bacterial species richness, with at least three distinct community types. We show that the deposit has clines of inorganic elements that can be leached by pH, which may be drivers of community structure. We also note the prevalence of Gallionellaceae in samples recovered near the surface, as well as taxa that include medically or economically important bacteria such as Actinomycetes and Paenibacillus These results provide insight into the microbial taxa that may be the source of KC antibacterial activity and suggest that natural clays may be rich sources of microbial and molecular diversity. IMPORTANCE Identifying and characterizing the resident microbial populations (bacteria, viruses, protozoa, and fungi) is key to understanding the ecology, chemistry, and homeostasis of virtually all sites on Earth. The Kisameet Bay deposit in British Columbia, Canada, holds a novel glacial clay with a history of medicinal use by local indigenous people. We previously showed that it has potent activity against a variety of antibiotic-resistant bacteria, suggesting it could complement our dwindling arsenal of antibiotics. Here, we have characterized the microbiome of this deposit to gain insight

  8. Investigation Of Information Sources And Communication Channels ...

    African Journals Online (AJOL)

    Investigation Of Information Sources And Communication Channels In Ipm Rice ... the information accessibility of farmer groups seems as empowerment strategy. ... information sources and communication channels, in order of importance, ...

  9. Investigation of bacterial repopulation after sinus surgery and perioperative antibiotics.

    Science.gov (United States)

    Hauser, Leah J; Ir, Diana; Kingdom, Todd T; Robertson, Charles E; Frank, Daniel N; Ramakrishnan, Vijay R

    2016-01-01

    Endoscopic sinus surgery (ESS) enjoys high success rates, but repopulation with pathogenic bacteria is 1 of the hallmarks of poorer outcomes. There are many hypothesized sources of repopulating bacteria; however, this process remains largely unexplored. This study examined changes in the sinus microbiome after ESS and medical therapies to identify potential sources for postsurgical microbial repopulation. Samples from the anterior nares, ethmoid sinus, and nasopharynx were taken at the time of surgery from 13 subjects undergoing ESS for chronic rhinosinusitis (CRS). Patients were treated postoperatively with 2 weeks of oral antibiotics and saline rinses. The ethmoid sinus was sampled at 2 and 6 weeks postoperatively; microbiota were characterized using quantitative polymerase chain reaction (qPCR) and 16S ribosomal RNA (rRNA) gene sequencing. The Morisita-Horn beta-diversity index (M-H) was used to compare similarity between samples. The bacterial burden of the ethmoid was higher 2 weeks postoperatively than 6 weeks postoperatively (p = 0.01). The 6-week samples most closely represented the anterior nares and ethmoid at surgery (M-H = 0.58 and 0.59, respectively), and were least similar to the nasopharynx (M-H = 0.28). Principal coordinates analysis (PCoA) plots illustrate that the ethmoid microbiota temporarily shifted after surgery and antibiotics but returned toward baseline in many subjects. Bacterial communities colonizing the ethmoid 6 weeks postoperatively were most similar to anterior nasal cavity and pretreatment sinus microbial profiles, indicating a high degree of resilience in the sinonasal microbiome of most subjects. Interestingly, surgery and postoperative antibiotic therapy does not appear to reduce bacterial burden, but rather, shifts the microbial consortia. © 2015 ARS-AAOA, LLC.

  10. A computer investigation of chemically mediated detachment in bacterial biofilms.

    Science.gov (United States)

    Hunt, Stephen M; Hamilton, Martin A; Sears, John T; Harkin, Gary; Reno, Jason

    2003-05-01

    A three-dimensional computer model was used to evaluate the effect of chemically mediated detachment on biofilm development in a negligible-shear environment. The model, BacLAB, combines conventional diffusion-reaction equations for chemicals with a cellular automata algorithm to simulate bacterial growth, movement and detachment. BacLAB simulates the life cycle of a bacterial biofilm from its initial colonization of a surface to the development of a mature biofilm with cell areal densities comparable to those in the laboratory. A base model founded on well established transport equations that are easily adaptable to investigate conjectures at the biological level has been created. In this study, the conjecture of a detachment mechanism involving a bacterially produced chemical detachment factor in which high local concentrations of this detachment factor cause the bacteria to detach from the biofilm was examined. The results show that the often observed 'mushroom'-shaped structure can occur if detachment events create voids so that the remaining attached cells look like mushrooms.

  11. Bacterial biofilms investigated by atomic force microscopy and electrochemistry

    DEFF Research Database (Denmark)

    Hu, Yifan

    Bacterial biofilms are aggregates of microorganisms in which cells adhere to each other and adhere to a solid surface or an animal host cavity. Bacterial biofilms play important roles in human life, and cause serious harm for human society and huge economic losses. The complex composition of bact...

  12. Bacterial composition in a metropolitan drinking water distribution system utilizing different source waters.

    Science.gov (United States)

    Gomez-Alvarez, Vicente; Humrighouse, Ben W; Revetta, Randy P; Santo Domingo, Jorge W

    2015-03-01

    We investigated the bacterial composition of water samples from two service areas within a drinking water distribution system (DWDS), each associated with a different primary source of water (groundwater, GW; surface water, SW) and different treatment process. Community analysis based on 16S rRNA gene clone libraries indicated that Actinobacteria (Mycobacterium spp.) and α-Proteobacteria represented nearly 43 and 38% of the total sequences, respectively. Sequences closely related to Legionella, Pseudomonas, and Vibrio spp. were also identified. In spite of the high number of sequences (71%) shared in both areas, multivariable analysis revealed significant differences between the GW and SW areas. While the dominant phylotypes where not significantly contributing in the ordination of samples, the populations associated with the core of phylotypes (1-10% in each sample) significantly contributed to the differences between both service areas. Diversity indices indicate that the microbial community inhabiting the SW area is more diverse and contains more distantly related species coexisting with local assemblages as compared with the GW area. The bacterial community structure of SW and GW service areas were dissimilar, suggesting that their respective source water and/or water quality parameters shaped by the treatment processes may contribute to the differences in community structure observed.

  13. Investigation of hydraulic transmission noise sources

    Science.gov (United States)

    Klop, Richard J.

    Advanced hydrostatic transmissions and hydraulic hybrids show potential in new market segments such as commercial vehicles and passenger cars. Such new applications regard low noise generation as a high priority, thus, demanding new quiet hydrostatic transmission designs. In this thesis, the aim is to investigate noise sources of hydrostatic transmissions to discover strategies for designing compact and quiet solutions. A model has been developed to capture the interaction of a pump and motor working in a hydrostatic transmission and to predict overall noise sources. This model allows a designer to compare noise sources for various configurations and to design compact and inherently quiet solutions. The model describes dynamics of the system by coupling lumped parameter pump and motor models with a one-dimensional unsteady compressible transmission line model. The model has been verified with dynamic pressure measurements in the line over a wide operating range for several system structures. Simulation studies were performed illustrating sensitivities of several design variables and the potential of the model to design transmissions with minimal noise sources. A semi-anechoic chamber has been designed and constructed suitable for sound intensity measurements that can be used to derive sound power. Measurements proved the potential to reduce audible noise by predicting and reducing both noise sources. Sound power measurements were conducted on a series hybrid transmission test bench to validate the model and compare predicted noise sources with sound power.

  14. Investigation of multimodal forward scatter phenotyping from bacterial colonies

    Science.gov (United States)

    Kim, Huisung

    A rapid, label-free, and elastic light scattering (ELS) based bacterial colony phenotyping technology, bacterial rapid detection using optical scattering technology (BARDOT) provides a successful classification of several bacterial genus and species. For a thorough understanding of the phenomena and overcoming the limitations of the previous design, five additional modalities from a bacterial colony: 3D morphology, spatial optical density (OD) distribution, spectral forward scattering pattern, spectral OD, and surface backward reflection pattern are proposed to enhance the classification/identification ratio, and the feasibilities of each modality are verified. For the verification, three different instruments: integrated colony morphology analyzer (ICMA), multi-spectral BARDOT (MS-BARDOT) , and multi-modal BARDOT (MM-BARDOT) are proposed and developed. The ICMA can measure 3D morphology and spatial OD distribution of the colony simultaneously. A commercialized confocal displacement meter is used to measure the profiles of the bacterial colonies, together with a custom built optical density measurement unit to interrogate the biophysics behind the collective behavior of a bacterial colony. The system delivers essential information related to the quantitative growth dynamics (height, diameter, aspect ratio, optical density) of the bacterial colony, as well as, a relationship in between the morphological characteristics of the bacterial colony and its forward scattering pattern. Two different genera: Escherichia coli O157:H7 EDL933, and Staphylococcus aureus ATCC 25923 are selected for the analysis of the spatially resolved growth dynamics, while, Bacillus spp. such as B. subtilis ATCC 6633, B. cereus ATCC 14579, B. thuringiensis DUP6044, B. polymyxa B719W, and B. megaterium DSP 81319, are interrogated since some of the Bacillus spp. provides strikingly different characteristics of ELS patterns, and the origin of the speckle patterns are successfully correlated with

  15. Investigation of In vitro Mineral Forming Bacterial Isolates from ...

    African Journals Online (AJOL)

    Aim: Although it is known that bacterial mechanisms are involved in dental calculus formation, which is a predisposing factor in periodontal diseases, there have been few studies of such associations, and therefore, information available is limited. The purpose of this study was to isolate and identify aerobic bacteria ...

  16. Modification of anti-bacterial surface properties of textile polymers by vacuum arc ion source implantation

    International Nuclear Information System (INIS)

    Nikolaev, A.G.; Yushkov, G.Yu.; Oks, E.M.; Oztarhan, A.; Akpek, A.; Hames-Kocabas, E.; Urkac, E.S.; Brown, I.G.

    2014-01-01

    Highlights: • Ion implantation. • Anti-bacterial properties. • Textile polymer. • Vacuum arc ion source. - Abstract: Ion implantation provides an important technology for the modification of material surface properties. The vacuum arc ion source is a unique instrument for the generation of intense beams of metal ions as well as gaseous ions, including mixed metal–gas beams with controllable metal:gas ion ratio. Here we describe our exploratory work on the application of vacuum arc ion source-generated ion beams for ion implantation into polymer textile materials for modification of their biological cell compatibility surface properties. We have investigated two specific aspects of cell compatibility: (i) enhancement of the antibacterial characteristics (we chose to use Staphylococcus aureus bacteria) of ion implanted polymer textile fabric, and (ii) the “inverse” concern of enhancement of neural cell growth rate (we chose Rat B-35 neuroblastoma cells) on ion implanted polymer textile. The results of both investigations were positive, with implantation-generated antibacterial efficiency factor up to about 90%, fully comparable to alternative conventional (non-implantation) approaches and with some potentially important advantages over the conventional approach; and with enhancement of neural cell growth rate of up to a factor of 3.5 when grown on suitably implanted polymer textile material

  17. Modification of anti-bacterial surface properties of textile polymers by vacuum arc ion source implantation

    Energy Technology Data Exchange (ETDEWEB)

    Nikolaev, A.G., E-mail: nik@opee.hcei.tsc.ru [High Current Electronics Institute, Siberian Branch of the Russian Academy of Sciences, Tomsk 634055 (Russian Federation); Yushkov, G.Yu.; Oks, E.M. [High Current Electronics Institute, Siberian Branch of the Russian Academy of Sciences, Tomsk 634055 (Russian Federation); Oztarhan, A. [Izmir University, Izmir 35140 (Turkey); Akpek, A.; Hames-Kocabas, E.; Urkac, E.S. [Bioengineering Department, Ege University, Bornova 35100, Izmir (Turkey); Brown, I.G. [Lawrence Berkeley National Laboratory, Berkeley, CA 94708 (United States)

    2014-08-15

    Highlights: • Ion implantation. • Anti-bacterial properties. • Textile polymer. • Vacuum arc ion source. - Abstract: Ion implantation provides an important technology for the modification of material surface properties. The vacuum arc ion source is a unique instrument for the generation of intense beams of metal ions as well as gaseous ions, including mixed metal–gas beams with controllable metal:gas ion ratio. Here we describe our exploratory work on the application of vacuum arc ion source-generated ion beams for ion implantation into polymer textile materials for modification of their biological cell compatibility surface properties. We have investigated two specific aspects of cell compatibility: (i) enhancement of the antibacterial characteristics (we chose to use Staphylococcus aureus bacteria) of ion implanted polymer textile fabric, and (ii) the “inverse” concern of enhancement of neural cell growth rate (we chose Rat B-35 neuroblastoma cells) on ion implanted polymer textile. The results of both investigations were positive, with implantation-generated antibacterial efficiency factor up to about 90%, fully comparable to alternative conventional (non-implantation) approaches and with some potentially important advantages over the conventional approach; and with enhancement of neural cell growth rate of up to a factor of 3.5 when grown on suitably implanted polymer textile material.

  18. Bacterial community diversity and variation in spray water sources and the tomato fruit surface.

    Science.gov (United States)

    Telias, Adriana; White, James R; Pahl, Donna M; Ottesen, Andrea R; Walsh, Christopher S

    2011-04-21

    Tomato (Solanum lycopersicum) consumption has been one of the most common causes of produce-associated salmonellosis in the United States. Contamination may originate from animal waste, insects, soil or water. Current guidelines for fresh tomato production recommend the use of potable water for applications coming in direct contact with the fruit, but due to high demand, water from other sources is frequently used. We sought to describe the overall bacterial diversity on the surface of tomato fruit and the effect of two different water sources (ground and surface water) when used for direct crop applications by generating a 454-pyrosequencing 16S rRNA dataset of these different environments. This study represents the first in depth characterization of bacterial communities in the tomato fruit surface and the water sources commonly used in commercial vegetable production. The two water sources tested had a significantly different bacterial composition. Proteobacteria was predominant in groundwater samples, whereas in the significantly more diverse surface water, abundant phyla also included Firmicutes, Actinobacteria and Verrucomicrobia. The fruit surface bacterial communities on tomatoes sprayed with both water sources could not be differentiated using various statistical methods. Both fruit surface environments had a high representation of Gammaproteobacteria, and within this class the genera Pantoea and Enterobacter were the most abundant. Despite the major differences observed in the bacterial composition of ground and surface water, the season long use of these very different water sources did not have a significant impact on the bacterial composition of the tomato fruit surface. This study has provided the first next-generation sequencing database describing the bacterial communities living in the fruit surface of a tomato crop under two different spray water regimes, and therefore represents an important step forward towards the development of science

  19. Bacterial community diversity and variation in spray water sources and the tomato fruit surface

    Directory of Open Access Journals (Sweden)

    Ottesen Andrea R

    2011-04-01

    Full Text Available Abstract Background Tomato (Solanum lycopersicum consumption has been one of the most common causes of produce-associated salmonellosis in the United States. Contamination may originate from animal waste, insects, soil or water. Current guidelines for fresh tomato production recommend the use of potable water for applications coming in direct contact with the fruit, but due to high demand, water from other sources is frequently used. We sought to describe the overall bacterial diversity on the surface of tomato fruit and the effect of two different water sources (ground and surface water when used for direct crop applications by generating a 454-pyrosequencing 16S rRNA dataset of these different environments. This study represents the first in depth characterization of bacterial communities in the tomato fruit surface and the water sources commonly used in commercial vegetable production. Results The two water sources tested had a significantly different bacterial composition. Proteobacteria was predominant in groundwater samples, whereas in the significantly more diverse surface water, abundant phyla also included Firmicutes, Actinobacteria and Verrucomicrobia. The fruit surface bacterial communities on tomatoes sprayed with both water sources could not be differentiated using various statistical methods. Both fruit surface environments had a high representation of Gammaproteobacteria, and within this class the genera Pantoea and Enterobacter were the most abundant. Conclusions Despite the major differences observed in the bacterial composition of ground and surface water, the season long use of these very different water sources did not have a significant impact on the bacterial composition of the tomato fruit surface. This study has provided the first next-generation sequencing database describing the bacterial communities living in the fruit surface of a tomato crop under two different spray water regimes, and therefore represents an

  20. A New Phylogenetic Approach to Investigate the Intestinal Bacterial Composition

    DEFF Research Database (Denmark)

    Hermann-Bank, Marie Louise; Mølbak, Lars

    literature, meaning their use had already been verified. DNA from reference bacteria and DNA from faecal samples were used to check specificity and sensitivity of the assay when analysing bacterial communities. The heat map showed nice dilution colour series consistent with the diluted sample concentrations...... reactions in a few hours. Subsequently the 16S rDNA amplicons can be harvested and used for next generation sequencing. The experimental setup was designed so that the primers represented a phylogenetic overview of the biggest groups of intestinal bacteria. Primers were mainly chosen from published...... ranging from 50 to 0.005 ng/μl and in agreement to the expected results. This implies that the AA48.48 is a useful tool when examining the bacterial composition of faeces and maybe superior to competitive methods, as it gives a quantitative measurement of the bacteria in the sample and afterwards...

  1. Investigation of bacterial diversity of hot springs of Odisha, India

    Directory of Open Access Journals (Sweden)

    Rajesh Kumar Sahoo

    2015-12-01

    Full Text Available 16S rRNA deep sequencing analysis, targeting V3 region was performed using Illumina bar coded sequencing. Sediment samples from two hot springs (Atri and Taptapani were collected. Atri and Taptapani metagenomes were classified into 50 and 51 bacterial phyla. Proteobacteria (45.17% dominated the Taptapani sample metagenome followed by Bacteriodetes (23.43% and Cyanobacteria (10.48% while in the Atri sample, Chloroflexi (52.39%, Nitrospirae (10.93% and Proteobacteria (9.98% dominated. A large number of sequences remained taxonomically unresolved in both hot springs, indicating the presence of potentially novel microbes in these two unique habitats thus unraveling the importance of the current study. Metagenome sequence information is now available at NCBI, SRA database accession no. SRP057428.

  2. Field investigations of bacterial contaminants and their effects on extended porcine semen.

    Science.gov (United States)

    Althouse, G C; Kuster, C E; Clark, S G; Weisiger, R M

    2000-03-15

    Field investigations (n=23) were made over a 3-yr period at North American boar studs and farms in which the primary complaint was sperm agglutination in association with decreased sperm longevity of extended semen, and increased regular returns to estrus and/or vaginal discharges across parity. Microscopic examination of extended semen from these units revealed depressed gross motility (usually semen collection and processing regardless of the semen extender used. The extended semen exhibited a high number of induced acrosome abnormalities (>20%). Sample pH was acidic (5.7 to 6.4) in 93% of the submitted samples. Aerobic culture yielded a variety of bacteria from different genera. A single bacterial contaminant was obtained from 66% of the submitted samples (n=37 doses); 34% contained 2 or more different bacterial genera. The most frequently isolated contaminant bacteria from porcine extended semen were Alcaligenes xylosoxydans (n=3), Burkholderia cepacia (n=6), Enterobacter cloacae (n=6), Escherichia coli (n=6), Serratia marcescens (n=5), and Stenotrophomonas [Xanthomonas] maltophilia (n=6); these 6 bacteria accounted for 71% of all contaminated samples, and were spermicidal when re-inoculated and incubated in fresh, high quality extended semen. All contaminant bacteria were found to be resistant to the aminoglycoside gentamicin, a common preservative antibiotic used in commercial porcine semen extenders. Eleven genera were spermicidal in conjunction with an acidic environment, while 2 strains (E. coli, S. maltophilia) were spermicidal without this characteristic acidic environment. Bacteria originated from multiple sources at the stud/farm, and were of animal and nonanimal origin. A minimum contamination technique (MCT) protocol was developed to standardize hygiene and sanitation. This protocol focused on MCT's during boar preparation, semen collection, semen processing and laboratory sanitation. Implementation of the MCT, in addition to specific recommendations

  3. Investigating source processes of isotropic events

    Science.gov (United States)

    Chiang, Andrea

    explosion. In contrast, recovering the announced explosive yield using seismic moment estimates from moment tensor inversion remains challenging but we can begin to put error bounds on our moment estimates using the NSS technique. The estimation of seismic source parameters is dependent upon having a well-calibrated velocity model to compute the Green's functions for the inverse problem. Ideally, seismic velocity models are calibrated through broadband waveform modeling, however in regions of low seismicity velocity models derived from body or surface wave tomography may be employed. Whether a velocity model is 1D or 3D, or based on broadband seismic waveform modeling or the various tomographic techniques, the uncertainty in the velocity model can be the greatest source of error in moment tensor inversion. These errors have not been fully investigated for the nuclear discrimination problem. To study the effects of unmodeled structures on the moment tensor inversion, we set up a synthetic experiment where we produce synthetic seismograms for a 3D model (Moschetti et al., 2010) and invert these data using Green's functions computed with a 1D velocity mode (Song et al., 1996) to evaluate the recoverability of input solutions, paying particular attention to biases in the isotropic component. The synthetic experiment results indicate that the 1D model assumption is valid for moment tensor inversions at periods as short as 10 seconds for the 1D western U.S. model (Song et al., 1996). The correct earthquake mechanisms and source depth are recovered with statistically insignificant isotropic components as determined by the F-test. Shallow explosions are biased by the theoretical ISO-CLVD tradeoff but the tectonic release component remains low, and the tradeoff can be eliminated with constraints from P wave first motion. Path-calibration to the 1D model can reduce non-double-couple components in earthquakes, non-isotropic components in explosions and composite sources and improve

  4. Sterile paper points as a bacterial DNA-contamination source in microbiome profiles of clinical samples

    NARCIS (Netherlands)

    van der Horst, J.; Buijs, M.J.; Laine, M.L.; Wismeijer, D.; Loos, B.G.; Crielaard, W.; Zaura, E.

    2013-01-01

    Objectives High throughput sequencing of bacterial DNA from clinical samples provides untargeted, open-ended information on the entire microbial community. The downside of this approach is the vulnerability to DNA contamination from other sources than the clinical sample. Here we describe

  5. Bacterial community composition and structure in an Urban River impacted by different pollutant sources.

    Science.gov (United States)

    Ibekwe, A Mark; Ma, Jincai; Murinda, Shelton E

    2016-10-01

    Microbial communities in terrestrial fresh water are diverse and dynamic in composition due to different environmental factors. The goal of this study was to undertake a comprehensive analysis of bacterial composition along different rivers and creeks and correlate these to land-use practices and pollutant sources. Here we used 454 pyrosequencing to determine the total bacterial community composition, and bacterial communities that are potentially of fecal origin, and of relevance to water quality assessment. The results were analyzed using UniFrac coupled with principal coordinate analysis (PCoA) to compare diversity, abundance, and community composition. Detrended correspondence analysis (DCA) and canonical correspondence analysis (CCA) were used to correlate bacterial composition in streams and creeks to different environmental parameters impacting bacterial communities in the sediment and surface water within the watershed. Bacteria were dominated by the phyla Proteobacteria, Bacteroidetes, Acidobacteria, and Actinobacteria, with Bacteroidetes significantly (PPCoA and DCA showed that bacterial composition in sediment and surface water was significantly different (Pmicrobial community compositions were influenced by several environmental factors, and pH, NO2, and NH4 were the major environmental factors driving FIB in surface water based on CCA analysis, while NO3 was the only factor in sediment. Published by Elsevier B.V.

  6. Investigation of bacterial hopanoid inputs to soils from Western Canada

    Energy Technology Data Exchange (ETDEWEB)

    Shunthirasingham, Chubashini [Department of Physical and Environmental Sciences, University of Toronto, Scarborough College, 1265 Military Trail, Toronto, Ont., M1C1A4 (Canada); Simpson, Myrna J. [Department of Physical and Environmental Sciences, University of Toronto, Scarborough College, 1265 Military Trail, Toronto, Ont., M1C1A4 (Canada)]. E-mail: myrna.simpson@utoronto.ca

    2006-06-15

    Hopanoids have been widely used as characteristic biomarkers to study inputs of bacterial biomass to sediments because they are preserved in the geologic record. A limited number of studies have been performed on hopanoid biomarkers in soils. The present study examined the distribution and potential preservation of hopanoids in soils that are developed under different climatic conditions and varying vegetative inputs. Solvent extraction and sequential chemical degradation methods were employed to extract both 'free' and 'bound' hopanoids, from three grassland soils, a grassland-forest transition soil, and a forest soil from Western Canada. Identification and quantification of hopanoids in the soil samples were carried out by gas chromatography-mass spectrometry. Methylbishomohopanol, bishomohopanol and bishomohopanoic acid were detected in all solvent extracts. The base hydrolysis and ruthenium tetroxide extracts contained only bishomohopanoic acid at a concentration range of 0.8-8.8 {mu}g/gC and 2.2-28.3 {mu}g/gC, respectively. The acid hydrolysis procedure did not release detectable amounts of hopanoids. The solvent extraction yielded the greatest amounts of 'free' hopanoids in two of the grassland soils (Dark Brown and Black Chernozems) and in the forest soil (Gray Luvisol). In contrast, the chemical degradation methods resulted in higher amounts of 'bound' hopanoids in the third grassland soil (Brown Chernozem) and the transition soil (Dark Gray Chernozem), indicating that more hopanoids exist in the 'bound' form in these soils. Overall, the forest and the transition soils contained more hopanoids than the grassland soils. This is hypothesized to be due to the greater degradation of hopanoids in the grassland soils and or sorption to clay minerals, as compared to the forest and transition soils.

  7. Investigation of bacterial hopanoid inputs to soils from Western Canada

    International Nuclear Information System (INIS)

    Shunthirasingham, Chubashini; Simpson, Myrna J.

    2006-01-01

    Hopanoids have been widely used as characteristic biomarkers to study inputs of bacterial biomass to sediments because they are preserved in the geologic record. A limited number of studies have been performed on hopanoid biomarkers in soils. The present study examined the distribution and potential preservation of hopanoids in soils that are developed under different climatic conditions and varying vegetative inputs. Solvent extraction and sequential chemical degradation methods were employed to extract both 'free' and 'bound' hopanoids, from three grassland soils, a grassland-forest transition soil, and a forest soil from Western Canada. Identification and quantification of hopanoids in the soil samples were carried out by gas chromatography-mass spectrometry. Methylbishomohopanol, bishomohopanol and bishomohopanoic acid were detected in all solvent extracts. The base hydrolysis and ruthenium tetroxide extracts contained only bishomohopanoic acid at a concentration range of 0.8-8.8 μg/gC and 2.2-28.3 μg/gC, respectively. The acid hydrolysis procedure did not release detectable amounts of hopanoids. The solvent extraction yielded the greatest amounts of 'free' hopanoids in two of the grassland soils (Dark Brown and Black Chernozems) and in the forest soil (Gray Luvisol). In contrast, the chemical degradation methods resulted in higher amounts of 'bound' hopanoids in the third grassland soil (Brown Chernozem) and the transition soil (Dark Gray Chernozem), indicating that more hopanoids exist in the 'bound' form in these soils. Overall, the forest and the transition soils contained more hopanoids than the grassland soils. This is hypothesized to be due to the greater degradation of hopanoids in the grassland soils and or sorption to clay minerals, as compared to the forest and transition soils

  8. Investigation of In vitro Mineral Forming Bacterial Isolates from ...

    African Journals Online (AJOL)

    2018-01-30

    Jan 30, 2018 ... Keywords: Bacteria, biomineralization, supragingival dental calculus. Investigation of In vitro ... of damage that is associated with plaque‑forming ... Materials and Methods ... Amplification reaction mixture was prepared in a 30 μL .... gingiva, palatine tonsils, and throat), skin (right and .... Neisseria mucosa.

  9. Exploring the effects of photon correlations from thermal sources on bacterial photosynthesis

    OpenAIRE

    Manrique, Pedro D.; Caycedo-Soler, Felipe; De Mendoza, Adriana; Rodríguez, Ferney; Quiroga, Luis; Johnson, Neil F.

    2016-01-01

    Thermal light sources can produce photons with strong spatial correlations. We study the role that these correlations might potentially play in bacterial photosynthesis. Our findings show a relationship between the transversal distance between consecutive absorptions and the efficiency of the photosynthetic process. Furthermore, membranes where the clustering of core complexes (so-called RC-LH1) is high, display a range where the organism profits maximally from the spatial correlation of the ...

  10. Investigation Of Information Sources And Communication Channels ...

    African Journals Online (AJOL)

    Extension of integrated pest management (IPM) as a component of sustainable agricultural development, involves empowering farmers. Facilitating the information accessibility of farmer groups seems as empowerment strategy. This strategy is based on identification of related patterns, including information sources and ...

  11. Investigating electrochemical removal of bacterial biofilms from stainless steel substrates.

    Science.gov (United States)

    Dargahi, Mahdi; Hosseinidoust, Zeinab; Tufenkji, Nathalie; Omanovic, Sasha

    2014-05-01

    Electrochemical removal of biofilms deserves attention because of its ease of use and environmentally friendly nature. We investigated the influence of electrode potential and treatment time on the removal of a 10-day old Pseudomonas aeruginosa biofilm formed on stainless steel 316 L substrates. At electrode potentials more positive than -1.5 V vs. Ag/AgCl, lower removal rates were observed and only partial removal of the biofilm was achieved during a 1-min time interval. Electrostatic repulsion between the film and electrode surface is believed to drive biofilm detachment under these conditions. However, when the biofilm-coated substrates were treated at potentials negative of -1.5 V vs. Ag/AgCl, complete removal of a biofilm was achieved within seconds. Under these conditions, vigorous evolution of hydrogen gas is believed to be responsible for the film removal, mechanically detaching the bacteria and extracellular polymeric matrix from the substrate. Stainless steel substrates were also subjected to repeated cycles of biofilm formation and electrochemical removal. High removal efficiencies were maintained throughout this process suggesting the potential of the proposed technology for application on conductive surfaces in various industrial settings. Copyright © 2014 Elsevier B.V. All rights reserved.

  12. Seaweed as source of energy. 1: effect of a specific bacterial strain on biogas production

    Energy Technology Data Exchange (ETDEWEB)

    Sreenivasa R.P.; Tarwade, S.J.; Sarma, K.S.R.

    1980-09-01

    Only certain marine bacteria capable of digesting the special type of polysaccharide - agar and alginic acid can bring about the biodegradation of these substances and utilise them as carbon source to produce the organics which will be utilised by the methane bacteria to produce methane. When bacterial strain was used in conjunction with cowdung as a source of methane bacteria in seaweed digester, production of biogas from seaweed was accelerated. Adding of small amount of Ulva to seaweed digester increased the output of gas. (Refs. 4).

  13. Recent innovation in microbial source tracking using bacterial real-time PCR markers in shellfish

    International Nuclear Information System (INIS)

    Mauffret, A.; Mieszkin, S.; Morizur, M.; Alfiansah, Y.; Lozach, S.; Gourmelon, M.

    2013-01-01

    Highlights: ► DNA extraction from intravalvular liquid is promising for microbial source tracking in oysters. ► Host-associated bacterial markers in shellfish digestive tissues were difficult to assess with real-time PCR. ► DNA extracts from shellfish flesh appeared to have low inhibitor levels but low marker levels. ► Protocol transfer from one shellfish species to another does not appear possible. -- Abstract: We assessed the capacity of real-time PCR markers to identify the origin of contamination in shellfish. Oyster, cockles or clams were either contaminated with fecal materials and host-associated markers designed from Bacteroidales or Catellicoccus marimammalium 16S RNA genes were extracted from their intravalvular liquid, digestive tissues or shellfish flesh. Extraction of bacterial DNA from the oyster intravalvular liquid with FastDNA spin kit for soil enabled the selected markers to be quantified in 100% of artificially contaminated samples, and the source of contamination to be identified in 13 out of 38 naturally contaminated batches from European Class B and Class C areas. However, this protocol did not enable the origin of the contamination to be identified in cockle or clam samples. Although results are promising for extracts from intravalvular liquid in oyster, it is unlikely that a single protocol could be the best across all bacterial markers and types of shellfish

  14. Investigators’ Guide to Sources of Information.

    Science.gov (United States)

    1997-04-01

    pathology, soil science, veterinary medicine , and zoology. A separate listing of book review citations follows the subject entries. Gale Directory of This...federal contracting, state and local government, and directories are listed in the index, subtopics such as counterfeiting , criminal history, income tax...responsible for investigating the counterfeiting of currency and securities; forgery and altering of government checks and bonds; thefts and fraud relating to

  15. Investigation of Solar and Solar-Gas Thermal Energy Sources

    OpenAIRE

    Ivan Herec; Jan Zupa

    2003-01-01

    The article deals with the investigation of solar thermal sources of electrical and heat energy as well as the investigation of hybrid solar-gas thermal sources of electrical and heat energy (so called photothermal sources). Photothermal sources presented here utilize computer-controlled injection of the conversion fluid into special capillary porous substance that is adjusted to direct temperature treatment by the concentrated thermal radiation absorption.

  16. Investigation of bacterial communities in peat land of the Gahai Lake natural conservation area

    Science.gov (United States)

    Bai, Yani; Wang, Jinchang; Zhan, Zhigao; Guan, Limei; Jin, Liang; Zheng, Guohua

    2017-10-01

    Peat is involved in the global carbon cycle and water conservation; therefore, it is implicated in global environmental change. Microorganisms play an important role in the function of peat. To investigate the bacterial communities in peat of Gahai Lake, different locations and depths were sampled and Illumina Miseq sequencing was used to analyze the microbial community. Chemical properties of peat samples were analyzed by China state standard methods (GB methods). The results showed that bacterial communities were affected by depth, with bacterial diversity and community structure at 90 and 120 cm significantly different from that at 10, 30 and 50 cm depth from the peat surface. Chemical properties of peat land including organic matter, total nitrogen and humus content did not significantly influence bacterial community structure in peat, with only one group from genus Rhizomicrobium that was significantly correlated with total nitrogen. A substantial proportion of the bacterial sequences were unclassified (1.4%), which indicates the great application potential of peat in the Gahai Lake natural conservation area in the future.

  17. Investigation of the bacterial communities associated with females of Lutzomyia sand fly species from South America.

    Directory of Open Access Journals (Sweden)

    Mauricio R V Sant'Anna

    Full Text Available Phlebotomine sand flies are vectors of Leishmania that are acquired by the female sand fly during blood feeding on an infected mammal. Leishmania parasites develop exclusively in the gut lumen during their residence in the insect before transmission to a suitable host during the next blood feed. Female phlebotomine sand flies are blood feeding insects but their life style of visiting plants as well as animals, and the propensity for larvae to feed on detritus including animal faeces means that the insect host and parasite are exposed to a range of microorganisms. Thus, the sand fly microbiota may interact with the developing Leishmania population in the gut. The aim of the study was to investigate and identify the bacterial diversity associated with wild adult female Lutzomyia sand flies from different geographical locations in the New World. The bacterial phylotypes recovered from 16S rRNA gene clone libraries obtained from wild caught adult female Lutzomyia sand flies were estimated from direct band sequencing after denaturing gradient gel electrophoresis of bacterial 16 rRNA gene fragments. These results confirm that the Lutzomyia sand flies contain a limited array of bacterial phylotypes across several divisions. Several potential plant-related bacterial sequences were detected including Erwinia sp. and putative Ralstonia sp. from two sand fly species sampled from 3 geographically separated regions in Brazil. Identification of putative human pathogens also demonstrated the potential for sand flies to act as vectors of bacterial pathogens of medical importance in addition to their role in Leishmania transmission.

  18. Exploring the effects of photon correlations from thermal sources on bacterial photosynthesis

    Directory of Open Access Journals (Sweden)

    Pedro D. Manrique

    Full Text Available Thermal light sources can produce photons with strong spatial correlations. We study the role that these correlations might potentially play in bacterial photosynthesis. Our findings show a relationship between the transversal distance between consecutive absorptions and the efficiency of the photosynthetic process. Furthermore, membranes where the clustering of core complexes (so-called RC-LH1 is high, display a range where the organism profits maximally from the spatial correlation of the incoming light. By contrast, no maximum is found for membranes with low core-core clustering. We employ a detailed membrane model with state-of-the-art empirical inputs. Our results suggest that the organization of the membrane’s antenna complexes may be well-suited to the spatial correlations present in an natural light source. Future experiments will be needed to test this prediction. Keywords: Photo-bunching, Spatial correlation, Photosynthesis, Purple bacteria

  19. Exploring the effects of photon correlations from thermal sources on bacterial photosynthesis

    Science.gov (United States)

    Manrique, Pedro D.; Caycedo-Soler, Felipe; De Mendoza, Adriana; Rodríguez, Ferney; Quiroga, Luis; Johnson, Neil F.

    Thermal light sources can produce photons with strong spatial correlations. We study the role that these correlations might potentially play in bacterial photosynthesis. Our findings show a relationship between the transversal distance between consecutive absorptions and the efficiency of the photosynthetic process. Furthermore, membranes where the clustering of core complexes (so-called RC-LH1) is high, display a range where the organism profits maximally from the spatial correlation of the incoming light. By contrast, no maximum is found for membranes with low core-core clustering. We employ a detailed membrane model with state-of-the-art empirical inputs. Our results suggest that the organization of the membrane's antenna complexes may be well-suited to the spatial correlations present in an natural light source. Future experiments will be needed to test this prediction.

  20. Study of the Bioremediation of Atrazine under Variable Carbon and Nitrogen Sources by Mixed Bacterial Consortium Isolated from Corn Field Soil in Fars Province of Iran

    Science.gov (United States)

    Nasseri, Simin; Hashemi, Hassan

    2013-01-01

    Atrazine herbicide that is widely used in corn production is frequently detected in water resources. The main objectives of this research were focused on assessing the effects of carbon and nitrogen sources on atrazine biodegradation by mixed bacterial consortium and by evaluating the feasibility of using mixed bacterial consortium in soil culture. Shiraz corn field soil with a long history of atrazine application has been explored for their potential of atrazine biodegradation. The influence of different carbon compounds and the effect of nitrogen sources and a different pH (5.5–8.5) on atrazine removal efficiency by mixed bacterial consortium in liquid culture were investigated. Sodium citrate and sucrose had the highest atrazine biodegradation rate (87.22%) among different carbon sources. Atrazine biodegradation rate decreased more quickly by the addition of urea (26.76%) compared to ammonium nitrate. Based on the data obtained in this study, pH of 7.0 is optimum for atrazine biodegradation. After 30 days of incubation, the percent of atrazine reduction rates were significantly enhanced in the inoculated soils (60.5%) as compared to uninoculated control soils (12%) at the soil moisture content of 25%. In conclusion, bioaugmentation of soil with mixed bacterial consortium may enhance the rate of atrazine degradation in a highly polluted soil. PMID:23533452

  1. Study of the Bioremediation of Atrazine under Variable Carbon and Nitrogen Sources by Mixed Bacterial Consortium Isolated from Corn Field Soil in Fars Province of Iran

    Directory of Open Access Journals (Sweden)

    Mansooreh Dehghani

    2013-01-01

    Full Text Available Atrazine herbicide that is widely used in corn production is frequently detected in water resources. The main objectives of this research were focused on assessing the effects of carbon and nitrogen sources on atrazine biodegradation by mixed bacterial consortium and by evaluating the feasibility of using mixed bacterial consortium in soil culture. Shiraz corn field soil with a long history of atrazine application has been explored for their potential of atrazine biodegradation. The influence of different carbon compounds and the effect of nitrogen sources and a different pH (5.5–8.5 on atrazine removal efficiency by mixed bacterial consortium in liquid culture were investigated. Sodium citrate and sucrose had the highest atrazine biodegradation rate (87.22% among different carbon sources. Atrazine biodegradation rate decreased more quickly by the addition of urea (26.76% compared to ammonium nitrate. Based on the data obtained in this study, pH of 7.0 is optimum for atrazine biodegradation. After 30 days of incubation, the percent of atrazine reduction rates were significantly enhanced in the inoculated soils (60.5% as compared to uninoculated control soils (12% at the soil moisture content of 25%. In conclusion, bioaugmentation of soil with mixed bacterial consortium may enhance the rate of atrazine degradation in a highly polluted soil.

  2. Seaweed as source of energy. I: effect of a specific bacterial strain on biogas production

    Energy Technology Data Exchange (ETDEWEB)

    Rao, P.S.; Tarwade, S.J.; Sarma, K.S.R.

    1980-01-01

    Biogas was produced from seaweed by making use of alginate-digesting marine bacteria that were isolated from decomposing seaweed and can digest seaweed carbohydrates (agar and alginic acid). Laboratory digesters containing 100 g seaweed were inoculated with 50 mL broth cultures of different seaweed-derived bacterial strains, and the maximum amount of degradation obtained was 28% (compared with 13% for a bacteria-free digestion). Cow dung was added as a source of methanogenic bacteria, and the amount of biogas produced was more than double the amount obtained when seaweed and cow dung were digested in the absence of the seaweed-derived bacteria. Adding a small amount of Ulva to the seaweed digester increased the production of biogas.

  3. International seminar on structural investigations on pulsed neutron sources. Proceedings

    International Nuclear Information System (INIS)

    Aksenov, V.L.; Balagurov, A.M.; Taran, Yu.V.

    1993-01-01

    The proceedings of the International seminar on structural investigations using pulsed neutron sources are presented. The seminar is dedicated to the memory of Dr. Yu.M. Ostanevich, a world acknowledged physicist. The problems of structural analysis using pulsed neutron source at the IBR-2 reactor are discussed

  4. Production of bacterial cellulose using different carbon sources and culture media.

    Science.gov (United States)

    Mohammadkazemi, Faranak; Azin, Mehrdad; Ashori, Alireza

    2015-03-06

    In this work, the effects of carbon sources and culture media on the production and structural properties of bacterial cellulose (BC) have been studied. BC nanofibers were synthesized using Gluconacetobacter xylinus strain PTCC 1734. Media used were Hestrin-Schramm (H), Yamanaka (Y), and Zhou (Z). Five different carbon sources, namely date syrup, glucose, mannitol, sucrose, and food-grade sucrose were used in these media. All the produced BC pellicles were characterized in terms of dry weight production, biomass yield, thermal stability, crystallinity and morphology by thermogravimetric analysis (TGA), x-ray diffraction (XRD), and field emission scanning electron microscopy (FE-SEM). The obtained results showed that mannitol lead to the highest yield, followed by sucrose. The highest production efficiency of mannitol might be due to the nitrogen source, which plays an important role. The maximum improvement on the thermal stability of the composites was achieved when mannitol was used in H medium. In addition, the crystallinity was higher in BC formed in H medium compared to other media. FE-SEM micrographs illustrated that the BC pellicles, synthesized in the culture media H and Z, were stable, unlike those in medium Y that were unstable. The micrographs of BC produced in media containing mannitol and sucrose provided evidence of the strong interfacial adhesion between the BC fibers without noticeable aggregates. Copyright © 2014 Elsevier Ltd. All rights reserved.

  5. Investigation of rf plasma light sources for dye laser excitation

    International Nuclear Information System (INIS)

    Kendall, J.S.; Jaminet, J.F.

    1975-06-01

    Analytical and experimental studies were performed to assess the applicability of radio frequency (rf) induction heated plasma light sources for potential excitation of continuous dye lasers. Experimental efforts were directed toward development of a continuous light source having spectral flux and emission characteristics approaching that required for pumping organic dye lasers. Analytical studies were performed to investigate (1) methods of pulsing the light source to obtain higher radiant intensity and (2) methods of integrating the source with a reflective cavity for pumping a dye cell. (TFD)

  6. Sequencing-Based Analysis of the Bacterial and Fungal Composition of Kefir Grains and Milks from Multiple Sources

    Science.gov (United States)

    Marsh, Alan J.; O’Sullivan, Orla; Hill, Colin; Ross, R. Paul; Cotter, Paul D.

    2013-01-01

    Kefir is a fermented milk-based beverage to which a number of health-promoting properties have been attributed. The microbes responsible for the fermentation of milk to produce kefir consist of a complex association of bacteria and yeasts, bound within a polysaccharide matrix, known as the kefir grain. The consistency of this microbial population, and that present in the resultant beverage, has been the subject of a number of previous, almost exclusively culture-based, studies which have indicated differences depending on geographical location and culture conditions. However, culture-based identification studies are limited by virtue of only detecting species with the ability to grow on the specific medium used and thus culture-independent, molecular-based techniques offer the potential for a more comprehensive analysis of such communities. Here we describe a detailed investigation of the microbial population, both bacterial and fungal, of kefir, using high-throughput sequencing to analyse 25 kefir milks and associated grains sourced from 8 geographically distinct regions. This is the first occasion that this technology has been employed to investigate the fungal component of these populations or to reveal the microbial composition of such an extensive number of kefir grains or milks. As a result several genera and species not previously identified in kefir were revealed. Our analysis shows that the bacterial populations in kefir are dominated by 2 phyla, the Firmicutes and the Proteobacteria. It was also established that the fungal populations of kefir were dominated by the genera Kazachstania, Kluyveromyces and Naumovozyma, but that a variable sub-dominant population also exists. PMID:23894461

  7. Sequencing-based analysis of the bacterial and fungal composition of kefir grains and milks from multiple sources.

    Directory of Open Access Journals (Sweden)

    Alan J Marsh

    Full Text Available Kefir is a fermented milk-based beverage to which a number of health-promoting properties have been attributed. The microbes responsible for the fermentation of milk to produce kefir consist of a complex association of bacteria and yeasts, bound within a polysaccharide matrix, known as the kefir grain. The consistency of this microbial population, and that present in the resultant beverage, has been the subject of a number of previous, almost exclusively culture-based, studies which have indicated differences depending on geographical location and culture conditions. However, culture-based identification studies are limited by virtue of only detecting species with the ability to grow on the specific medium used and thus culture-independent, molecular-based techniques offer the potential for a more comprehensive analysis of such communities. Here we describe a detailed investigation of the microbial population, both bacterial and fungal, of kefir, using high-throughput sequencing to analyse 25 kefir milks and associated grains sourced from 8 geographically distinct regions. This is the first occasion that this technology has been employed to investigate the fungal component of these populations or to reveal the microbial composition of such an extensive number of kefir grains or milks. As a result several genera and species not previously identified in kefir were revealed. Our analysis shows that the bacterial populations in kefir are dominated by 2 phyla, the Firmicutes and the Proteobacteria. It was also established that the fungal populations of kefir were dominated by the genera Kazachstania, Kluyveromyces and Naumovozyma, but that a variable sub-dominant population also exists.

  8. Sequencing-based analysis of the bacterial and fungal composition of kefir grains and milks from multiple sources.

    Science.gov (United States)

    Marsh, Alan J; O'Sullivan, Orla; Hill, Colin; Ross, R Paul; Cotter, Paul D

    2013-01-01

    Kefir is a fermented milk-based beverage to which a number of health-promoting properties have been attributed. The microbes responsible for the fermentation of milk to produce kefir consist of a complex association of bacteria and yeasts, bound within a polysaccharide matrix, known as the kefir grain. The consistency of this microbial population, and that present in the resultant beverage, has been the subject of a number of previous, almost exclusively culture-based, studies which have indicated differences depending on geographical location and culture conditions. However, culture-based identification studies are limited by virtue of only detecting species with the ability to grow on the specific medium used and thus culture-independent, molecular-based techniques offer the potential for a more comprehensive analysis of such communities. Here we describe a detailed investigation of the microbial population, both bacterial and fungal, of kefir, using high-throughput sequencing to analyse 25 kefir milks and associated grains sourced from 8 geographically distinct regions. This is the first occasion that this technology has been employed to investigate the fungal component of these populations or to reveal the microbial composition of such an extensive number of kefir grains or milks. As a result several genera and species not previously identified in kefir were revealed. Our analysis shows that the bacterial populations in kefir are dominated by 2 phyla, the Firmicutes and the Proteobacteria. It was also established that the fungal populations of kefir were dominated by the genera Kazachstania, Kluyveromyces and Naumovozyma, but that a variable sub-dominant population also exists.

  9. Linkage of the Nit1C gene cluster to bacterial cyanide assimilation as a nitrogen source.

    Science.gov (United States)

    Jones, Lauren B; Ghosh, Pallab; Lee, Jung-Hyun; Chou, Chia-Ni; Kunz, Daniel A

    2018-05-21

    A genetic linkage between a conserved gene cluster (Nit1C) and the ability of bacteria to utilize cyanide as the sole nitrogen source was demonstrated for nine different bacterial species. These included three strains whose cyanide nutritional ability has formerly been documented (Pseudomonas fluorescens Pf11764, Pseudomonas putida BCN3 and Klebsiella pneumoniae BCN33), and six not previously known to have this ability [Burkholderia (Paraburkholderia) xenovorans LB400, Paraburkholderia phymatum STM815, Paraburkholderia phytofirmans PsJN, Cupriavidus (Ralstonia) eutropha H16, Gluconoacetobacter diazotrophicus PA1 5 and Methylobacterium extorquens AM1]. For all bacteria, growth on or exposure to cyanide led to the induction of the canonical nitrilase (NitC) linked to the gene cluster, and in the case of Pf11764 in particular, transcript levels of cluster genes (nitBCDEFGH) were raised, and a nitC knock-out mutant failed to grow. Further studies demonstrated that the highly conserved nitB gene product was also significantly elevated. Collectively, these findings provide strong evidence for a genetic linkage between Nit1C and bacterial growth on cyanide, supporting use of the term cyanotrophy in describing what may represent a new nutritional paradigm in microbiology. A broader search of Nit1C genes in presently available genomes revealed its presence in 270 different bacteria, all contained within the domain Bacteria, including Gram-positive Firmicutes and Actinobacteria, and Gram-negative Proteobacteria and Cyanobacteria. Absence of the cluster in the Archaea is congruent with events that may have led to the inception of Nit1C occurring coincidentally with the first appearance of cyanogenic species on Earth, dating back 400-500 million years.

  10. Investigation of the Rate of the Bacterial Contamination of the Ice Factories in Bandar Abbas, Iran

    Directory of Open Access Journals (Sweden)

    Nahid Moradi

    2016-09-01

    Full Text Available Background: Pollution of drinking water and ice is one of the most serious ways of water borne diseases spread. The purpose of this study was to investigate the bacterial contamination of the ice produced by ice factories in Bandar Abbas. Methods: In this descriptive ,cross-sectional study samples were collected from seven ice factories in Bandar Abbas. Sampling was done by standard method. Amount of the Contamination of ice and water to coliforms was investigated by the Multiple Tube method to determine the MPN, isolate bacteria and identify the microorganisms using conventional bacteriological techniques and counting the total count of bacteria by the Plate Count method on a nutrient agar medium. The data was analyzed by the SPSS software. Results: In this study, a total of 84 samples were investigated. Gram-positive bacteria (65.5% and gram-negative bacteria(34.5% were separated from each other. The MPN rate in samples and total count of bacteria were 0->1100 and 2×101×104 CFU/mL, respectively. Conclusion: The results suggest that necessary precautions be taken by environmental health specialists and other public health authorities in production, transportation and distribution of the ice blocks to reduce the rate of bacterial contamination.

  11. Medicinal plants - a potent antibacterial source against bacterial leaf blight (BLB) of rice

    International Nuclear Information System (INIS)

    Jabeen, R.

    2011-01-01

    The antibacterial potential of indigenous medicinal plants as alternative chemical pesticides for controlling bacterial leaf blight (BLB) of rice was investigated. Twenty-five different species of medicinal plants were collected from various sites in Pakistan. Decoctions of all medicinal plant species were screened by the disc plate diffusion method for testing the susceptibility of an aggressive isolate of Xanthomonas oryzae pv. oryzae (Xoo 105). Out of twenty five medicinal plants, Thuja orientalis (cone + leaves), Azadirachta indica (seeds + fruits), Amomum subulatum (fruits), Terminalia chebula (fruits), Terminalia bellirica (fruits), Anethum graveolens (fruits) and Ferula assa-foetida (fruits) decoctions showed significant activity. The efficacy of decoctions from six promising plants were further tested through detached leaf, glasshouse and field assays. A decoction of Terminalia chebula demonstrated the highest effectiveness in terms of regulating BLB in the plants both under laboratory and field conditions. Bioactive fractions of Terminalia chebula were purified, characterized and tentatively identified as allegic acid. (author)

  12. Utilization of Glyphosate as Phosphate Source: Biochemistry and Genetics of Bacterial Carbon-Phosphorus Lyase

    Science.gov (United States)

    Zechel, David L.; Jochimsen, Bjarne

    2014-01-01

    SUMMARY After several decades of use of glyphosate, the active ingredient in weed killers such as Roundup, in fields, forests, and gardens, the biochemical pathway of transformation of glyphosate phosphorus to a useful phosphorus source for microorganisms has been disclosed. Glyphosate is a member of a large group of chemicals, phosphonic acids or phosphonates, which are characterized by a carbon-phosphorus bond. This is in contrast to the general phosphorus compounds utilized and metabolized by microorganisms. Here phosphorus is found as phosphoric acid or phosphate ion, phosphoric acid esters, or phosphoric acid anhydrides. The latter compounds contain phosphorus that is bound only to oxygen. Hydrolytic, oxidative, and radical-based mechanisms for carbon-phosphorus bond cleavage have been described. This review deals with the radical-based mechanism employed by the carbon-phosphorus lyase of the carbon-phosphorus lyase pathway, which involves reactions for activation of phosphonate, carbon-phosphorus bond cleavage, and further chemical transformation before a useful phosphate ion is generated in a series of seven or eight enzyme-catalyzed reactions. The phn genes, encoding the enzymes for this pathway, are widespread among bacterial species. The processes are described with emphasis on glyphosate as a substrate. Additionally, the catabolism of glyphosate is intimately connected with that of aminomethylphosphonate, which is also treated in this review. Results of physiological and genetic analyses are combined with those of bioinformatics analyses. PMID:24600043

  13. Effects of alternative energy sources on bacterial cellulose characteristics produced by Komagataeibacter medellinensis.

    Science.gov (United States)

    Molina-Ramírez, Carlos; Enciso, Carla; Torres-Taborda, Mabel; Zuluaga, Robin; Gañán, Piedad; Rojas, Orlando J; Castro, Cristina

    2018-05-27

    Bacterial cellulose (BC) was produced by Komagataeibacter medellinensis using Hestrin and Schramm modified medium in the presence of alternative energy sources (AES), such as ethanol and acetic acid, to explore the effect of AES on the characteristics and properties of the resulting BC. In this study, the physicochemical and structural characteristics of the obtained BC were determined using Fourier-transform infrared spectroscopy, X-ray diffraction spectrometry, thermogravimetric analysis, and mechanical testing analysis. Ethanol and acetic acid (at 0.1 wt%) were proven to improve the BC yield by K. medellinensis by 279% and 222%, respectively. However, the crystallinity index (%), the degree of polymerization, and maximum rate of degradation temperatures decreased by 9.2%, 36%, and 4.96%, respectively, by the addition of ethanol and by 7.2%, 27%, and 4.21%, respectively, by the addition of acetic acid. The significance of this work, lies on the fact that there is not any report about how BC properties change when substances like ethanol or acetic acid are added to culture medium, and which is the mechanism that provokes those changes, that in our case we could demonstrate the relationship of a higher BC production rate (provoked by ethanol and acetic acid adding) and changes in BC properties. Copyright © 2018 Elsevier B.V. All rights reserved.

  14. Bacterial source tracking guides management of boat head waste in a coastal resort area.

    Science.gov (United States)

    Mallin, Michael A; Haltom, Mary I; Song, Bongkeun; Tavares, Mary E; Dellies, Stephen P

    2010-12-01

    Fecal contamination of water bodies causes a public health problem and economic loss. To control such contamination management actions need to be guided by sound science. From 2007-2009 a study was undertaken to determine the sources of fecal bacteria contamination to the marine waters adjoining the Town of Wrightsville Beach, North Carolina, USA. The research effort included sampling for fecal coliform and Enterococcus bacteria, sampling for optical brighteners, dye studies, and use of molecular bacterial source tracking techniques including polymerase chain reaction (PCR) and terminal restriction fragment polymorphism (T-RFLP) fingerprinting of the Bacteroides-Prevotella group. Of the 96 samples collected from nine locations during the study, the water contact standard for Enterococcus was exceeded on 13 occasions. The T-RFLP fingerprint analyses demonstrated that the most widespread source of fecal contamination was human, occurring in 38% of the samples, with secondary ruminant and avian sources also detected. Optical brightener concentrations were low, reflecting a lack of sewage line leakage or spills. A lack of sewer leaks and lack of septic systems in the town pointed toward discharge from boat heads into the marine waters as the major cause of fecal contamination; this was supported by dye studies. Based on these data, the Town initiated action to have the U.S. Environmental Protection Agency declare the coastal waters (out to 3 nautical miles), the nearby Atlantic Intracoastal Waterway and its tributaries a no-discharge zone (NDZ) to alleviate the human fecal pollution. The Town garnered supporting resolutions from other local communities who jointly petitioned the North Carolina Department of Environmental and Natural Resources. This State regulatory agency supported the local government resolutions and sent an application for an NDZ to the EPA in April 2009. The EPA concurred, and in February 2010 the coastal waters of New Hanover County, NC, became the

  15. Glyphosate Shapes a Dinoflagellate-Associated Bacterial Community While Supporting Algal Growth as Sole Phosphorus Source

    Directory of Open Access Journals (Sweden)

    Cong Wang

    2017-12-01

    Full Text Available Glyphosate is a widely used herbicide that can potentially be a phosphorus (P source for phytoplankton and microbes when discharged into the coastal ocean. In contrast to bacteria, few eukaryotic phytoplankton species appear capable of directly utilizing glyphosate. In this study, we observed, after a long delay (>60 days, Prorocentrum donghaiense, a dinoflagellate known to cause major harmful algal blooms in the East China Sea, could grow in a medium with glyphosate as the sole P source; suggesting that P. donghaiense growth was through bacterial mediation. To understand how the bacteria community might respond to glyphosate, we analyzed the 16S rRNA genes of the microbial community present in P. donghaiense cultures when grown under lower (36 μM and higher (360 μM glyphosate concentrations. Based on both Sanger and Illumina high throughput sequencing, we obtained more than 55,323 good-quality sequences, which were classified into six phyla. As the concentration of glyphosate rose, our results showed a significant increase in the phyla Proteobacteria and Firmicutes and a decrease in the phylum Bacteroidetes. Further qPCR (Quantitative PCR analysis showed higher abundances of two specific phylotypes in the higher-glyphosate P. donghaiense cultures when compared to the lower-glyphosate and no-glyphosate cultures. Correspondingly, qPCR displayed the same trend for the abundance of a gammaproteobacterial type of phnJ, a gene encoding Alpha-D-ribose 1-methylphosphonate 5-phosphate C-P lyase, which is responsible for phosphonate degradation. In addition, Tax4Fun analysis based on our 16S rRNA gene sequences results in higher predicted abundances of phosphonate metabolizing genes in glyphosate-treated cultures. This study demonstrates that glyphosate could selectively promote the growth of particular groups of bacteria within an algal culture and in glyphosate enriched coastal waters, this interaction may potentially further facilitate the growth of

  16. Micro Corona Ionizer as an Ozone Source for Bacterial Cell Lysis

    Science.gov (United States)

    Lee, Eun-Hee; Lim, Hyun Jeong; Chua, Beelee; Son, Ahjeong

    2015-04-01

    DNA extraction is a critical process of DNA assays including polymerase chain reaction (PCR), microarrays, molecular cloning, and DNA hybridization which has been well established and can be implemented by commercial kits. DNA extraction involves cell lysis, precipitation, and purification through the combination of physical and chemical processes. Cell lysis is essential to high DNA recovery yield which can be achieved via a variety of physical, chemical, and enzymatic methods. However, these methods were originally developed for bioassays that were labor intensive, time consuming, and vulnerable to contamination and inhibition. Here, we proposed to employ a micro corona ionizer as an ozone source to lyse bacterial cells. Ozone has been well known and used as a disinfectant which allows cell lysis and DNA extraction. Previously, we have shown that a micro corona ionizer is capable of generating a significant amount of ozone. In this study, we employed the micro corona ionizer for the bacterial cell lysis which consists of a 50 μm diameter cantilever wire as the discharge cathode and a 50 μm thick copper foil as anode. Applied voltages varied from 1900 to 2200 V with corresponding corona currents from 16 to 28 μA. The resultant ozone (concentration > 0.14 ppm) generated from the micro corona ionizer was bubbled into the sample via a miniature pump. We demonstrated the cell lysis of Pseudomonas putida as the target bacterium using the micro corona ionizer. At a flow rate of 38 ml/min and applied corona voltage of 2000 V, 98.5 ± 0.2% lysis (normalized to sonication result) was achieved after 10 min. In comparison, untreated and air-treated samples showed normalized % lysis of 11.9 ± 2.4 and 36.1 ± 1.7%, respectively. We also showed that the cell lysis efficiency could be significantly increased by increasing the flow rate and the applied corona voltage. By comparing the experimental results for continuous and pulsed treatment, we verified that the percentage of

  17. Investigation of an incident due to orphan sources in Iran

    Energy Technology Data Exchange (ETDEWEB)

    Mehdizadeh, S. [Shiraz Univ. (Iran, Islamic Republic of); Kardan, M.R.; Abdollah Miangi, F.; Rastkhah, N. [National Radiation Protection Dept., Tehran (Iran, Islamic Republic of)

    2006-07-01

    This paper discusses an incident occurred in one of the radiation application centers in Iran, and follow up investigations as well as lessons learnt. In January 2004 the Regulatory Authority was informed through Shiraz University R.P.O. of an incident regarding orphan radioactive sources which belonged to a Radiation Application Center (R.A.C.) in Shiraz. In this incident a metallic box containing a neutron emitting and three gamma emitting sources belonging to the above mentioned center were unearthed from a burial site outside of Shiraz by some person or persons and the box was eventually set on fire. Investigations conducted later on, showed that despite the melting of the paraffin shield of neutron emitting source, the capsules containing sources were intact and unharmed, and no radioactive leaking had occurred. Further investigations showed that this box contained one Am-Be source and three Cs-137 sources which had been given to the above mentioned center, long time before by a foreign well logging company from former Czech Republic and without notifying the Regulatory Authority. Follow up measurements indicated the maximum dose rate of 111 GBq Am-Be and 3.7 GBq Cs-137 sources to be 13 mSv/h and 7 mSv/hr accordingly. Therefore the maximum dose for the people involved in this incident is estimated to have been about 100 mSv, consequently no severe deterministic effects to individuals is expected: the findings showed that the main reasons for the incident were as follows: 1. Lack of professionalism in working with radioactive materials. 2. Violation of obligation under Radiation protection act and related regulations by the owner of the sources which was a well logging foreign company. 3. Leaving the sources in an improper storage condition. 4. Unauthorized access to the radiation sources in the owner center. 5. Lack of an effective national register system in the Regulatory Authority. 6. Lack of a well scheduled and risk base inspection program for the

  18. Bacterial Species and Biochemical Characteristic Investigations of Nostoc flagelliforme Concentrates during its Storage.

    Science.gov (United States)

    Yue, Lifang; Lv, Hexin; Zhen, Jing; Jiang, Shengping; Jia, Shiru; Shen, Shigang; Gao, Lu; Dai, Yujie

    2016-04-28

    Preservation of fresh algae plays an important role in algae seed subculture and aquaculture. The determination and examination of the changes of cell viability, composition, and bacterial species during storage would help to take suitable preservation methods to prolong the preservation time of fresh algae. Nostoc flagelliforme is a kind of edible cyanobacterium with important herbal and dietary values. This article investigated the changes of bacterial species and biochemical characteristics of fresh N. flagelliforme concentrate during natural storage. It was found that the viability of cells decreased along with the storage time. Fourteen bacteria strains in the algae concentrate were identified by PCR-DGGE and were grouped into four phyla, including Cyanobacteria, Firmicutes, Proteobacteria, and Bacteroidetes. Among them, Enterococcus viikkiensis may be a concern in the preservation. Eleven volatile organic compounds were identified from N. flagelliforme cells, in which geosmin could be treated as an indicator of the freshness of N. flagelliforme. The occurrence of indole compound may be an indicator of the degradation of cells.

  19. Investigating source confusion in PMN J1603-4904

    Science.gov (United States)

    Krauß, F.; Kreter, M.; Müller, C.; Markowitz, A.; Böck, M.; Burnett, T.; Dauser, T.; Kadler, M.; Kreikenbohm, A.; Ojha, R.; Wilms, J.

    2018-02-01

    PMN J1603-4904 is a likely member of the rare class of γ-ray emitting young radio galaxies. Only one other source, PKS 1718-649, has been confirmed so far. These objects, which may transition into larger radio galaxies, are a stepping stone to understanding AGN evolution. It is not completely clear how these young galaxies, seen edge-on, can produce high-energy γ rays. PMN J1603-4904 has been detected by TANAMI Very Long Baseline Interferometry (VLBI) observations and has been followed-up with multiwavelength observations. A Fermi Gamma-ray Space Telescope Large Area Telescope (Fermi-LAT) γ-ray source has been associated with this young galaxy in the LAT catalogs. We have obtained Chandra observations of the source to consider the possibility of source confusion due to the relatively large positional uncertainty of Fermi-LAT. The goal was to investigate the possibility of other X-ray bright sources in the vicinity of PMN J1603-4904 that could be counterparts to the γ-ray emission. With Chandra/ACIS, we find no other sources in the uncertainty ellipse of Fermi-LAT data, which includes an improved localization analysis of eight years of data. We further study the X-ray fluxes and spectra. We conclude that PMN J1603-4904 is indeed the second confirmed γ-ray bright young radio galaxy.

  20. Molecular characterization and potential sources of aqueous humor bacterial contamination during phacoemulsification with intraocular lens implantation in dogs.

    Science.gov (United States)

    Lacerda, Luciana C C; de Souza-Pollo, Andressa; Padua, Ivan Ricardo M; Conceição, Luciano F; da Silveira, Camila P Balthazar; Silva, Germana A; Maluta, Renato P; Laus, José L

    2018-01-01

    Bacterial contamination of the anterior chamber during cataract surgery is one of the main responsible for endophthalmitis postoperative. Phacoemulsification is a less invasive technique for cataract treatment, although it does not exclude the possibility of contamination. In this study, bacterial contaminants of aqueous humor collected pre- and post-phacoemulsification with intraocular lens implantation (IOL) of twenty dogs were identified. As the conjunctival microbiota constitute a significant source of anterior chamber contamination, bacterial isolates from aqueous humor were genetically compared with those present in the conjunctival surface of the patients. Three dogs presented bacterial growth in both aqueous humor and conjunctival surface samples. Bacterial isolates from these samples were grouped according to their genetic profiles by repetitive-element PCR (rep-PCR) and their representatives were identified by 16S rRNA sequencing. Isolates from conjunctival surface were identified as Enterobacter spp., Staphylococcus spp. and S. aureus; and from aqueous humor samples as Enterobacter spp., Pantoea spp., Streptococcus spp. and Staphylococcus spp., respectively in decreasing order of prevalence. According to the rep-PCR analysis, 16.6% of Enterobacter spp. isolates from conjunctival surface were genetically similar to those from aqueous humor. The rest of isolates encountered in aqueous humor were genetically distinct from those of conjunctival surface. The significant genetic diversity of bacterial isolates found in the aqueous humor samples after surgery denoted the possibility of anterior chamber contamination during phacoemulsification by bacteria not only from conjunctival surface but also from different sources related to surgical environment. Copyright © 2017 Elsevier B.V. All rights reserved.

  1. Phase I Source Investigation, Heckathorn Superfund Site, Richmond, California

    Energy Technology Data Exchange (ETDEWEB)

    Kohn, Nancy P; Evans, Nathan R

    2002-12-18

    This report represents Phase I of a multi-phase approach to a source investigation of DDT at the Heckathorn Superfund Site, Richmond, California, the former site of a pesticide packaging plant, and the adjacent waterway, the Lauritzen Channel. Potential identified sources of contamination were from sloughed material from undredged areas (such as side banks) and from outfall pipes. Objectives of Phase I included the (1) evaluation of pesticide concentrations associated with discharge from outfalls, (2) identification of additional outfalls in the area, (3) identification of type, quantity, and distribution of sediment under the Levin pier, (4) quantification of pesticide concentrations in sediment under the pier, and (5) evaluation of sediment structure and slope stability under the pier. Field operations included the collection of sediment directly from inside the mouths of outfall pipes, when possible, or the deployment of specially designed particle traps where direct sampling was problematic. Passive water samplers were placed at the end of known outfall pipes and analyzed for DDT and other pesticides of concern. Underwater dive surveys were conducted beneath the Levin pier to document type, slope, and thickness of sediment. Samples were collected at locations of interest and analyzed for contaminants. Also sampled was soil from bank areas, which were suspected of potentially contributing to continued DDT contamination of the Lauritzen Channel through erosion and groundwater leaching. The Phase I Source Investigation was successful in identifying significant sources of DDT contamination to Lauritzen Channel sediment. Undredged sediment beneath the Levin pier that has been redistributed to the channel is a likely source. Two outfalls tested bear further investigation. Not as well-defined are the contributions of bank erosional material and groundwater leaching. Subsequent investigations will be based on the results of this first phase.

  2. Fabrication and investigation of a biocompatible microfilament with high mechanical performance based on regenerated bacterial cellulose and bacterial cellulose.

    Science.gov (United States)

    Wu, Huan-Ling; Bremner, David H; Wang, Hai-Jun; Wu, Jun-Zi; Li, He-Yu; Wu, Jian-Rong; Niu, Shi-Wei; Zhu, Li-Min

    2017-10-01

    A high-strength regenerated bacterial cellulose (RBC)/bacterial cellulose (BC) microfilament of potential use as a biomaterial was successfully prepared via a wet spinning process. The BC not only consists of a 3-D network composed of nanofibers with a diameter of several hundred nanometers but also has a secondary structure consisting of highly oriented nanofibrils with a diameter ranging from a few nanometers to tens of nanometers which explains the reason for the high mechanical strength of BC. Furthermore, a strategy of partially dissolving BC was used and this greatly enhanced the mechanical performance of spun filament and a method called post-treatment was utilized to remove residual solvents from the RBC/BC filaments. A comparison of structure, properties, as well as cytocompatibility between BC nanofibers and RBC/BC microfilaments was achieved using morphology, mechanical properties, X-ray Diffraction (XRD) and an enzymatic hydrolysis assay. The RBC/BC microfilament has a uniform groove structure with a diameter of 50-60μm and XRD indicated that the crystal form was transformed from cellulose Iα to cellulose III I and the degree of crystallinity of RBC/BC (33.22%) was much lower than the original BC (60.29%). The enzymatic hydrolysis assay proved that the RBC/BC material was more easily degraded than BC. ICP detection indicated that the residual amount of lithium was 0.07mg/g (w/w) and GC-MS analysis showed the residual amount of DMAc to be 8.51μg/g (w/w) demonstrating that the post-treatment process is necessary and effective for removal of residual materials from the RBC/BC microfilaments. Also, a cell viability assay demonstrated that after post-treatment the RBC/BC filaments had good cytocompatibility. Copyright © 2017 Elsevier B.V. All rights reserved.

  3. Investigation of bacterial adherence to a non-precious alloy with radiolabeling method

    International Nuclear Information System (INIS)

    Sonugelen, M.; Iyiyapici Destan, U.; Oeztuerk, B.; Yurt Lambrecht, F.

    2006-01-01

    The objective of this study was to investigate the bacterial adherence to a non-precious alloy with radiolabeling method. S. mutans, E. coliand C. albicanswere labeled with 99m Tc by using stannous chloride and their radiolabeling yields were calculated. After the labeling procedure, metal disks (3 mm x 10 mm) were treated with microorganisms. The amount of labeled microorganisms adhered on metal surfaces was determined by activity measurements. The labeling yields for S. mutans, E. coliand C. albicanswere 69.95 ± 7.58%, 78.84 ± 0.44% and 79.71 ± 10.17%, respectively. The mean values for adherence for S. mutans, E. coliand C. albicans on metal samples were 7.02 ± 2.18%, 0.96 ± 0.49% and 8.80 ± 8.24%, respectively. The radiolabeling method could be considered as safe and precise for determining the adherence of microorganisms. (author)

  4. Production of Bacterial Cellulose by Gluconacetobacter hansenii Using Corn Steep Liquor As Nutrient Sources

    Directory of Open Access Journals (Sweden)

    Andrea F. S. Costa

    2017-10-01

    Full Text Available Cellulose is mainly produced by plants, although many bacteria, especially those belonging to the genus Gluconacetobacter, produce a very peculiar form of cellulose with mechanical and structural properties that can be exploited in numerous applications. However, the production cost of bacterial cellulose (BC is very high to the use of expensive culture media, poor yields, downstream processing, and operating costs. Thus, the purpose of this work was to evaluate the use of industrial residues as nutrients for the production of BC by Gluconacetobacter hansenii UCP1619. BC pellicles were synthesized using the Hestrin–Schramm (HS medium and alternative media formulated with different carbon (sugarcane molasses and acetylated glucose and nitrogen sources [yeast extract, peptone, and corn steep liquor (CSL]. A jeans laundry was also tested. None of the tested sources (beside CSL worked as carbon and nutrient substitute. The alternative medium formulated with 1.5% glucose and 2.5% CSL led to the highest yield in terms of dry and hydrated mass. The BC mass produced in the alternative culture medium corresponded to 73% of that achieved with the HS culture medium. The BC pellicles demonstrated a high concentration of microfibrils and nanofibrils forming a homogenous, compact, and three-dimensional structure. The biopolymer produced in the alternative medium had greater thermal stability, as degradation began at 240°C, while degradation of the biopolymer produced in the HS medium began at 195°C. Both biopolymers exhibited high crystallinity. The mechanical tensile test revealed the maximum breaking strength and the elongation of the break of hydrated and dry pellicles. The dry BC film supported up to 48 MPa of the breaking strength and exhibited greater than 96.98% stiffness in comparison with the hydrated film. The dry film supported up to 48 MPa of the breaking strength and exhibited greater than 96.98% stiffness in comparison with the hydrated film

  5. Starting material radiation source for Moessbauer investigations of tellurium compounds

    International Nuclear Information System (INIS)

    Alexandrov, A.J.; Grushko, J.S.; Makarov, E.F.; Mishin, K.Y.; Baltrunas, D.A.J.

    1977-01-01

    A method is described of preparing a radiation source for Mossbauer investigations of tellurium compounds manufactured on the basis of 5 MgO . Te 124 O 3 . 5 MgO . Te 124 O 3 is irradiated in a reactor by means of thermal neutrons, followed by annealing at a temperature ranging from 600 0 to 1,100 0 C for a period of from 5 to 10 hours

  6. Investigation of bacterial diversity in the feces of cattle fed different diets.

    Science.gov (United States)

    Kim, M; Kim, J; Kuehn, L A; Bono, J L; Berry, E D; Kalchayanand, N; Freetly, H C; Benson, A K; Wells, J E

    2014-02-01

    The objective of this study is to investigate individual animal variation of bovine fecal microbiota including as affected by diets. Fecal samples were collected from 426 cattle fed 1 of 3 diets typically fed to feedlot cattle: 1) 143 steers fed finishing diet (83% dry-rolled corn, 13% corn silage, and 4% supplement), 2) 147 steers fed late growing diet (66% dry-rolled corn, 26% corn silage, and 8% supplement), and 3) 136 heifers fed early growing diet (70% corn silage and 30% alfalfa haylage). Bacterial 16S rRNA gene amplicons were determined from individual fecal samples using next-generation pyrosequencing technology. A total of 2,149,008 16S rRNA gene sequences from 333 cattle with at least 2,000 sequences were analyzed. Firmicutes and Bacteroidetes were dominant phyla in all fecal samples. At the genus level, Oscillibacter, Turicibacter, Roseburia, Fecalibacterium, Coprococcus, Clostridium, Prevotella, and Succinivibrio were represented by more than 1% of total sequences. However, numerous sequences could not be assigned to a known genus. Dominant unclassified groups were unclassified Ruminococcaceae and unclassified Lachnospiraceae that could be classified to a family but not to a genus. These dominant genera and unclassified groups differed (P < 0.001) with diets. A total of 176,692 operational taxonomic units (OTU) were identified in combination across all the 333 cattle. Only 2,359 OTU were shared across 3 diet groups. UniFrac analysis showed that bacterial communities in cattle feces were greatly affected by dietary differences. This study indicates that the community structure of fecal microbiota in cattle is greatly affected by diet, particularly between forage- and concentrate-based diets.

  7. Thermal Performance and Efficiency Investigation of Conventional Boost, Z-source and Y-source Converters

    DEFF Research Database (Denmark)

    Gadalla, Brwene Salah Abdelkarim; Schaltz, Erik; Siwakoti, Yam Prasad

    2016-01-01

    Boost converters are needed in many applications that require the output voltage to be higher than the input voltage. Recently, boost type converters have been attracted by the industrial applications, and hence it has become an extremely hot topic of research. Recently, many researchers proposed...... the impedance source converters with their unique advantages as having a high voltage gain in a small range of duty cycle ratio. However, the thermal behaviour of the semiconductor devices and passive elements in the impedance source converter is an important issue from a reliability point of view and has...... not been investigated yet. Therefore this paper presents a comparison between the conventional boost, the Z-source, and the Y-source converters based on the thermal evaluation of semiconductors. In addition, the three topologies are also compared with respect to their efficiency. The operational principle...

  8. Investigating the Antimicrobial Bioactivity of Cyano bacterial Extracts on Some Plant and Human Pathogens

    International Nuclear Information System (INIS)

    El-Semary, N.A.; Osman, M.E.; Ahmed, A.S.; Botros, H.W.; Farag, A.T.

    2014-01-01

    The search for broad spectrum antimicrobial agents against microbial pathogens, as the available bioactive compounds, has decreasing efficacy and the multidrug resistance trait is spreading among pathogens. Accordingly, the study was carried out to investigate the antimicrobial bioactivity of extracts derived from a cyano bacterial strain from Egypt. The solvents used were diethyl ether, chloroform and methanol. The antimicrobial bioassay of the lipophilic fraction dissolved in diethyl ether of Synechococcus spp. (isolated from Wadi El-Natroun, Egypt) showed the highest broad spectrum bioactivity as it inhibited the growth of both plant and human pathogens. The extract was also effective on the filamentous plant pathogenic fungi Aspergillus flavus and Aspergillus niger. The effects of incubation periods, growth media and pH values on both growth and antimicrobial activity of Synechococcus spp. were investigated. Chu medium was the medium that gave the highest growth followed by BG11 medium then Oscillatoria medium and all these three media showed antibacterial activities but only BG11 showed both antibacterial and antifungal activities after 18 days of incubation. The pH value 10 proved to be the best for growth and antimicrobial activities of Synechococcus spp. in BG11 medium

  9. Response of soil bacterial communities to lead and zinc pollution revealed by Illumina MiSeq sequencing investigation.

    Science.gov (United States)

    Xu, Xihui; Zhang, Zhou; Hu, Shunli; Ruan, Zhepu; Jiang, Jiandong; Chen, Chen; Shen, Zhenguo

    2017-01-01

    Soil provides a critical environment for microbial community development. However, microorganisms may be sensitive to substances such as heavy metals (HMs), which are common soil contaminants. This study investigated bacterial communities using 16S ribosomal RNA (rRNA) gene fragment sequencing in geographic regions with and without HM pollution to elucidate the effects of soil properties and HMs on bacterial communities. No obvious changes in the richness or diversity of bacterial communities were observed between samples from mining and control areas. Significant differences in bacterial richness and diversity were detected between samples from different geographic regions, indicating that the basic soil characteristics were the most important factors affecting bacterial communities other than HMs. However, the abundances of several phyla and genera differed significantly between mining and control samples, suggesting that Zn and Pb pollution may impact the soil bacterial community composition. Moreover, regression analyses showed that the relative abundances of these phyla and genera were correlated significantly with the soil-available Zn and Pb contents. Redundancy analysis indicated that the soil K, ammoniacal nitrogen (NH 4 + -N), total Cu, and available Zn and Cu contents were the most important factors. Our results not only suggested that the soil bacteria were sensitive to HM stresses but also indicated that other soil properties may affect soil microorganisms to a greater extent.

  10. A kinetic Monte Carlo approach to investigate antibiotic translocation through bacterial porins

    International Nuclear Information System (INIS)

    Ceccarelli, Matteo; Ruggerone, Paolo; Vargiu, Attilio V

    2012-01-01

    Many relevant biological processes take place on time scales not reachable by standard all-atom computer simulations. The translocation of antibiotics through non-specific bacterial porins is an example. Microscopic effects compete to determine penetration routes and, consequently, free energy barriers to be overcome. Since bacteria can develop resistance to treatment also by reducing their antibiotic permeability, to understand the microscopic aspects of antibiotic translocation is an important step to rationalize drug design. Here, to investigate the translocation we propose a complete numerical model that combines the diffusion-controlled rate theory and a kinetic Monte Carlo scheme based on both experimental data and microscopically well-founded all-atom simulations. Within our model, an antibiotic translocating through an hour-glass-shaped channel can be described as a molecule moving on a potential of mean force featuring several affinity sites and a high central barrier. The implications of our results for the characterization of antibiotic translocation at in vivo concentrations are discussed. The presence of an affinity site close to the mouth of the channel seems to favor the translocation of antibiotics, the affinity site acting as a particle reservoir. Possible connections between results and the appearance of mutations in clinical strains are also outlined. (paper)

  11. Investigation of the source of residual phthalate in sundried salt.

    Science.gov (United States)

    Kim, Jin Hyo; Lee, Jin Hwan; Kim, So-Young

    2014-03-01

    Phthalate contamination in sundried salt has recently garnered interest in Korea. Phthalate concentrations were investigated in Korean sundried salts, source waters, and aqueous extracts from polyvinyl chloride materials used in salt ponds. Preliminary screening results for phthalates in Korean sundried salts revealed that only di(2-ethylhexyl)phthalate (DEHP) was over the limit of detection, with an 8.6% detection rate, and the concentration ranged from below the limit of detection to 0.189 mg/kg. The tolerable daily intake contribution ratio of the salt was calculated to be only 0.001%. Residual phthalates were below 0.026 mg/liter in source water, and the aqueous extracted di-n-butylphthalate, benzylbutylphthalate, and DEHP, which are considered endocrine disruptors, were below 0.029 mg/kg as derived from the polyvinyl chloride materials in salt ponds. The transfer ratios of the six phthalates from seawater to sundried salts were investigated; transfer ratio was correlated with vapor pressure (r(2) = 0.9875). Thus, di-n-butylphthalate, benzylbutylphthalate, DEHP, and di-n-octylphthalate can be considered highly likely residual pollutants in some consumer salts.

  12. An investigation of total bacterial communities, culturable antibiotic-resistant bacterial communities and integrons in the river water environments of Taipei city.

    Science.gov (United States)

    Yang, Chu-Wen; Chang, Yi-Tang; Chao, Wei-Liang; Shiung, Iau-Iun; Lin, Han-Sheng; Chen, Hsuan; Ho, Szu-Han; Lu, Min-Jheng; Lee, Pin-Hsuan; Fan, Shao-Ning

    2014-07-30

    The intensive use of antibiotics may accelerate the development of antibiotic-resistant bacteria (ARB). The global geographical distribution of environmental ARB has been indicated by many studies. However, the ARB in the water environments of Taiwan has not been extensively investigated. The objective of this study was to investigate the communities of ARB in Huanghsi Stream, which presents a natural acidic (pH 4) water environment. Waishuanghsi Stream provides a neutral (pH 7) water environment and was thus also monitored to allow comparison. The plate counts of culturable bacteria in eight antibiotics indicate that the numbers of culturable carbenicillin- and vancomycin-resistant bacteria in both Huanghsi and Waishuanghsi Streams are greater than the numbers of culturable bacteria resistant to the other antibiotics tested. Using a 16S rDNA sequencing approach, both the antibiotic-resistant bacterial communities (culture-based) and the total bacterial communities (metagenome-based) in Waishuanghsi Stream exhibit a higher diversity than those in Huanghsi Stream were observed. Of the three classes of integron, only class I integrons were identified in Waishuanghsi Stream. Our results suggest that an acidic (pH 4) water environment may not only affect the community composition of antibiotic-resistant bacteria but also the horizontal gene transfer mediated by integrons. Copyright © 2013 Elsevier B.V. All rights reserved.

  13. Physical therapy clinic therapeutic ultrasound equipment as a source for bacterial contamination.

    Science.gov (United States)

    Spratt, Henry G; Levine, David; Tillman, Larry

    2014-10-01

    A procedure commonly used in physical therapy (PT) clinics is therapeutic ultrasound (US). This equipment and associated gel comes in contact with patient skin, potentially serving as a reservoir for bacteria. In this study, we sampled US heads, gel bottle tips and gel from nine outpatient PT clinics in Southeastern Tennessee. Samples were collected using sterile swabs. At the microbiology laboratory, these swabs were used to inoculate mannitol salt agar and CHROM-MRSA agar (for Staphylococcal species) and tryptic soy broth to determine non-specific bacterial contamination. US heads, gel bottle tips and gel had variable levels of contamination. Tips of gel bottles had the highest contamination, with 52.7% positive for non-specific bacterial contamination and 3.6% positive for methicillin-resistant Staphylococcus aureus (MRSA). Contamination of gel by non-specific bacteria was found in 14.5% of bottles sampled. US heads (35.5% of those sampled) had non-specific bacterial contamination, with no MRSA detected. Disinfecting US heads after initial swabbing resulted in removal of 90.9% of non-specific contamination. Gel storage at temperatures below 40 °C was found to encourage the growth of mesophilic bacteria. This study demonstrates the need for better cleaning and storage protocols for US heads and gel bottles in PT clinics.

  14. Investigations on a hybrid positron source with a granular converter

    Energy Technology Data Exchange (ETDEWEB)

    Artru, X. [Institut de Physique Nucleaire de Lyon, Universite Lyon 1, CNRS/IN2P3, Villeurbanne (France); Chaikovska, I. [Laboratoire de l’Accelerateur Lineaire (LAL), Universite Paris-Sud, Bat. 200, 91898 Orsay (France); Chehab, R., E-mail: chehab@lal.in2p3.fr [Institut de Physique Nucleaire de Lyon, Universite Lyon 1, CNRS/IN2P3, Villeurbanne (France); Chevallier, M. [Institut de Physique Nucleaire de Lyon, Universite Lyon 1, CNRS/IN2P3, Villeurbanne (France); Dadoun, O. [Laboratoire de l’Accelerateur Lineaire (LAL), Universite Paris-Sud, Bat. 200, 91898 Orsay (France); Furukawa, K. [Accelerator Laboratory (KEK), Oho, Tsukuba, Ibaraki 305-0801 (Japan); Guler, H. [Laboratoire de l’Accelerateur Lineaire (LAL), Universite Paris-Sud, Bat. 200, 91898 Orsay (France); Kamitani, T.; Miyahara, F.; Satoh, M. [Accelerator Laboratory (KEK), Oho, Tsukuba, Ibaraki 305-0801 (Japan); Sievers, P. [CERN, Geneva (Switzerland); Suwada, T.; Umemori, K. [Accelerator Laboratory (KEK), Oho, Tsukuba, Ibaraki 305-0801 (Japan); Variola, A. [Laboratoire de l’Accelerateur Lineaire (LAL), Universite Paris-Sud, Bat. 200, 91898 Orsay (France)

    2015-07-15

    Promising results obtained with crystal targets for positron production led to the elaboration of a hybrid source made of an axially oriented tungsten crystal, as a radiator, and an amorphous tungsten converter. If the converter is granular, made of small spheres, the heat dissipation is greatly enhanced and the thermal shocks reduced, allowing the consideration of such device for the future linear colliders. A positron source of this kind is investigated. Previous simulations have shown very promising results for the yield as for the energy deposition and the PEDD (Peak Energy Deposition Density). Here, we present detailed simulations made in this granular converter with emphasis on the energy deposition density, which is a critical parameter as learned from the breakdown of the SLC target. A test on the KEKB linac is foreseen; it will allow a determination of the energy deposited and the PEDD in the converter through temperature measurements. Four granular converters, made of W spheres of mm radius have been built at LAL-Orsay; they will be installed at KEK and compared to compact converters. A description of the experimental layout at KEK is provided. Applications to future linear colliders as CLIC and ILC are considered.

  15. Investigating goal conflict as a source of mixed emotions.

    Science.gov (United States)

    Berrios, Raul; Totterdell, Peter; Kellett, Stephen

    2015-01-01

    This research investigated whether (1) the experience of mixed emotions is a consequence of activating conflicting goals and (2) mixed emotions are distinct from emotional conflict. A preliminary experiment (Study 1, N = 35) showed that an elicited goal conflict predicted more mixed emotions than a condition where the same goals were not in conflict. The second experiment was based on naturally occurring goal activation (Study 2, N = 57). This illustrated that mixed emotions were experienced more following conflicting goals compared with a facilitating goals condition-on both a direct self-report measure of mixed emotions and a minimum index measure. The results also showed that mixed emotions were different to emotional conflict. Overall, goal conflict was found to be a source of mixed emotions, and it is feasible that such states have a role in resolving personal dilemmas.

  16. Exploring the sources of bacterial spoilers in beefsteaks by culture-independent high-throughput sequencing.

    Directory of Open Access Journals (Sweden)

    Francesca De Filippis

    Full Text Available Microbial growth on meat to unacceptable levels contributes significantly to change meat structure, color and flavor and to cause meat spoilage. The types of microorganisms initially present in meat depend on several factors and multiple sources of contamination can be identified. The aims of this study were to evaluate the microbial diversity in beefsteaks before and after aerobic storage at 4°C and to investigate the sources of microbial contamination by examining the microbiota of carcasses wherefrom the steaks originated and of the processing environment where the beef was handled. Carcass, environmental (processing plant and meat samples were analyzed by culture-independent high-throughput sequencing of 16S rRNA gene amplicons. The microbiota of carcass swabs was very complex, including more than 600 operational taxonomic units (OTUs belonging to 15 different phyla. A significant association was found between beef microbiota and specific beef cuts (P<0.01 indicating that different cuts of the same carcass can influence the microbial contamination of beef. Despite the initially high complexity of the carcass microbiota, the steaks after aerobic storage at 4°C showed a dramatic decrease in microbial complexity. Pseudomonas sp. and Brochothrix thermosphacta were the main contaminants, and Acinetobacter, Psychrobacter and Enterobacteriaceae were also found. Comparing the relative abundance of OTUs in the different samples it was shown that abundant OTUs in beefsteaks after storage occurred in the corresponding carcass. However, the abundance of these same OTUs clearly increased in environmental samples taken in the processing plant suggesting that spoilage-associated microbial species originate from carcasses, they are carried to the processing environment where the meat is handled and there they become a resident microbiota. Such microbiota is then further spread on meat when it is handled and it represents the starting microbial association

  17. Investigation of noise sources and propagation in external gear pumps

    Science.gov (United States)

    Opperwall, Timothy J.

    Oil hydraulics is widely accepted as the best technology for transmitting power in many engineering applications due to its advantages in power density, control, layout flexibility, and efficiency. Due to these advantages, hydraulic systems are present in many different applications including construction, agriculture, aerospace, automotive, forestry, medical, and manufacturing, just to identify a few. Many of these applications involve the systems in close proximity to human operators and passengers where noise is one of the main constraints to the acceptance and spread of this technology. As a key component in power transfer, displacement machines can be major sources of noise in hydraulic systems. Thus, investigation into the sources of noise and discovering strategies to reduce noise is a key part of applying fluid power systems to a wider range of applications, as well as improving the performance of current hydraulic systems. The present research aims to leverage previous efforts and develop new models and experimental techniques in the topic of noise generation caused by hydrostatic units. This requires challenging and surpassing current accepted methods in the understanding of noise in fluid power systems. This research seeks to expand on the previous experimental and modeling efforts by directly considering the effect that system and component design changes apply on the total sound power and the sound frequency components emitted from displacement machines and the attached lines. The case of external gear pumps is taken as reference for a new model to understand the generation and transmission of noise from the sources out to the environment. The lumped parameter model HYGESim (HYdraulic GEar machine Simulator) was expanded to investigate the dynamic forces on the solid bodies caused by the pump operation and to predict interactions with the attached system. Vibration and sound radiation were then predicted using a combined finite element and boundary

  18. Investigation of the effects of oleuropein rich diet on rat enteric bacterial flora.

    Science.gov (United States)

    Kiraz, A; Simsek, T; Tekin, S Z; Elmas, S; Tekin, M; Sahin, H; Altinisik, H B; Pala, C

    2016-01-01

    Oleuropein is a phenolic compound of olive leaves. Enteric bacterial flora is very important for human health and diet is a directly affecting factor of enteric bacterial flora composition. In this study, it was hypothesized that oleuropein could reduce total aerobic bacterial count in rat caecal flora. Twenty adult, male, Wistar albino rats were randomly divided into two groups. Group C (n=10) was fed with standard rat chow and water for 30 days. Group O (n=10) received olive leaf extract 20 mg/kg/day by intragastric gavage in addition to standard rat chow and water for 30 days. One gram of caecal content was collected from each rat and then consecutive 10-fold serial dilutions were prepared with a final concentration of 10-8. Then 0.1 ml of each dilution were spread onto the surfaces of Plate Count Agar and Violet Red Bile Glucose Agar to enumerate the aerobic enteric bacteria. Total aerobic bacterial counts of Group O were significantly lower than of Group C in all agar plates inoculated with ceacal samples for every dilution (pbacterial translocation by reducing enteric bacterial counts (Tab. 1, Ref. 32).

  19. An investigation into the bacterial contamination of goniolenses in use in clinical practice.

    Science.gov (United States)

    Grundon, Rachael; Scurrell, Emma; Mould, John; Hayton-Lee, Emma; Heinrich, Christine

    2017-12-18

    Objectives To report the incidence and evaluate the clinical significance of goniolens bacterial contamination in clinical use in dogs with three different usage protocols and one with an added cleaning protocol. Animals Studied and Methods Three groups of twenty dogs undergoing gonioscopy at a private practice in the UK had the goniolenses swabbed for bacteriology culture and identification prior to placement on the cornea. Three protocols of lens use, with 2 different types of goniolens, were studied. One protocol was then repeated with 21 dogs with a lens cleaning protocol prior to storage. Results Low levels of bacterial contamination were found in all 3 initial groups (10-15%). No correlation was found between usage protocol used and rate of contamination and no correlation was found between length of storage between use and contamination. All bacteria cultured were considered naturally occurring commensals for the canine eye and environment. The group with a cleaning protocol had a 4.7% contamination rate. This was not statistically different from the non-cleaning groups. Conclusions The rate of bacterial contamination of goniolenses in clinical practice is low and the bacterial contaminants consist of commensal bacteria, unlikely to be of detriment to the eye. Minimal contamination of the goniolenses was found and this did not appear to be of clinical significance. The introduction of a simple cleaning protocol did not produce a statistically significant reduction in bacterial contamination. © 2017 American College of Veterinary Ophthalmologists.

  20. Nitrate source apportionment using a combined dual isotope, chemical and bacterial property, and Bayesian model approach in river systems

    Science.gov (United States)

    Xia, Yongqiu; Li, Yuefei; Zhang, Xinyu; Yan, Xiaoyuan

    2017-01-01

    Nitrate (NO3-) pollution is a serious problem worldwide, particularly in countries with intensive agricultural and population activities. Previous studies have used δ15N-NO3- and δ18O-NO3- to determine the NO3- sources in rivers. However, this approach is subject to substantial uncertainties and limitations because of the numerous NO3- sources, the wide isotopic ranges, and the existing isotopic fractionations. In this study, we outline a combined procedure for improving the determination of NO3- sources in a paddy agriculture-urban gradient watershed in eastern China. First, the main sources of NO3- in the Qinhuai River were examined by the dual-isotope biplot approach, in which we narrowed the isotope ranges using site-specific isotopic results. Next, the bacterial groups and chemical properties of the river water were analyzed to verify these sources. Finally, we introduced a Bayesian model to apportion the spatiotemporal variations of the NO3- sources. Denitrification was first incorporated into the Bayesian model because denitrification plays an important role in the nitrogen pathway. The results showed that fertilizer contributed large amounts of NO3- to the surface water in traditional agricultural regions, whereas manure effluents were the dominant NO3- source in intensified agricultural regions, especially during the wet seasons. Sewage effluents were important in all three land uses and exhibited great differences between the dry season and the wet season. This combined analysis quantitatively delineates the proportion of NO3- sources from paddy agriculture to urban river water for both dry and wet seasons and incorporates isotopic fractionation and uncertainties in the source compositions.

  1. Investigation and usage of renewable energy sources. First conference proceedings

    International Nuclear Information System (INIS)

    Tiit, Valdur

    2000-01-01

    The conference was devoted to sustainable development promotion in Estonia. Modern lifestyle and outstanding technical achievements are strongly based on usage of fossil energy sources, especially oil products. Development demands an increasing amount of energy, but the supplies of non-renewable natural resources are limited. Moreover, their usage pollutes the environment and conveys vital oxygen out of the atmosphere. Due to supplies run out the production of fossil fuels will inevitably decrease already after 20 years. The same is likely to happen with Estonian oil shale resources, which is not only a fuel for electric power stations, but also an important staple of chemical industry. And after the rise of oil prices and its fall in market share, oil shale will have even greater value. To satisfy mankind's energy demand we have to take solar energy and processes started by solar (wind, flowing water, synthesis of plants biomass) more into use. The spread of their usage is determined by natural and social situation of the region, and economic expediency. Although rapid growth in using renewable energy sources has started, it will take decades to raise their share over half of total energy use. Estonia has chosen the path of sustainable development, which should guarantee development and healthy environment also in the farther future. There are great solar and wind energy resources plus good assumptions for producing biomass in Estonia. However, for efficient use of national natural resources we need more knowledge and skills, people with environment friendly attitude and extensive scientific, technical and applied investigations. In addition we have to advance sensible international cooperation as well as national industry of this field. The technical progress concerning renewable energy usage has a long way to go, which could apply also Estonians

  2. Investigation of critical inter-related factors affecting the efficacy of pulsed light for inactivating clinically relevant bacterial pathogens.

    Science.gov (United States)

    Farrell, H P; Garvey, M; Cormican, M; Laffey, J G; Rowan, N J

    2010-05-01

    To investigate critical electrical and biological factors governing the efficacy of pulsed light (PL) for the in vitro inactivation of bacteria isolated from the clinical environment. Development of this alternative PL decontamination approach is timely, as the incidence of health care-related infections remains unacceptably high. Predetermined cell numbers of clinically relevant Gram-positive and Gram-negative bacteria were inoculated separately on agar plates and were flashed with lamp discharge energy (range 3.2-20 J per pulse), the amount of pulsing applied (range 0-60 pulses) and the distance between light source and treatment surface (range 8-20 cm) used. Greater decontamination levels were achieved using a combination of higher lamp discharge energies, increased number of pulses and shorter distances between treatment surface and the xenon light source. Levels of microbial sensitivity also varied depending on the population type, size and age of cultures treated. Production of pigment pyocynanin and alginate slime in mucoid strains of Pseudomonas aeruginosa afforded some protection against lethal action of PL; however, this was evident only by using a combination of reduced amount of pulsing at the lower lamp discharge energies tested. A clear pattern was observed where Gram-positive bacterial pathogens were more resistant to cidal effects of PL compared to Gram negatives. While negligible photoreactivation of PL-treated bacterial strains occurred after full pulsing regimes at the different lamp discharge energies tested, some repair was evident when using a combination of reduced pulsing at the lower lamp discharge energies. Strains harbouring genes for multiple resistances to antibiotics were not significantly more resistant to PL treatments. Slight temperature rises (lamp discharge energies. Presence of organic matter on treatment surface did not significantly affect PL decontamination efficacy, nor did growth of PL-treated bacteria on selective agar

  3. Behind Linus's Law: Investigating Peer Review Processes in Open Source

    Science.gov (United States)

    Wang, Jing

    2013-01-01

    Open source software has revolutionized the way people develop software, organize collaborative work, and innovate. The numerous open source software systems that have been created and adopted over the past decade are influential and vital in all aspects of work and daily life. The understanding of open source software development can enhance its…

  4. A New Experimental Design for Bacterial Microleakage Investigation at the Implant-Abutment Interface: An In Vitro Study.

    Science.gov (United States)

    Zipprich, Holger; Miatke, Sven; Hmaidouch, Rim; Lauer, Hans-Christoph

    2016-01-01

    regarding the bacterial seal of different implant systems. Conical IACs offer a better bacterial seal compared with flat IACs, which showed increased microleakage after dynamic loading. IAC design plays a crucial role in terms of bacterial colonization. Taking samples of the implant interior without abutment disconnection eliminates an error source.

  5. Investigation of 6-[¹⁸F]-fluoromaltose as a novel PET tracer for imaging bacterial infection.

    Directory of Open Access Journals (Sweden)

    Gayatri Gowrishankar

    Full Text Available Despite advances in the field of nuclear medicine, the imaging of bacterial infections has remained a challenge. The existing reagents suffer from poor sensitivity and specificity. In this study we investigate the potential of a novel PET (positron emission tomography tracer that overcomes these limitations.6-[¹⁸F]-fluoromaltose was synthesized. Its behavior in vitro was evaluated in bacterial and mammalian cultures. Detailed pharmacokinetic and biodistribution profiles for the tracer were obtained from a murine model.6-[¹⁸F]-fluoromaltose is taken up by multiple strains of pathogenic bacteria. It is not taken up by mammalian cancer cell lines. 6-[¹⁸F]-fluoromaltose is retained in infected muscles in a murine model of bacterial myositis. It does not accumulate in inflamed tissue.We have shown that 6-[¹⁸F]-fluoromaltose can be used to image bacterial infection in vivo with high specificity. We believe that this class of agents will have a significant impact on the clinical management of patients.

  6. Investigation of spore forming bacterial flooding for enhanced oil recovery in a North Sea chalk Reservoir

    DEFF Research Database (Denmark)

    Halim, Amalia Yunita; Nielsen, Sidsel Marie; Eliasson Lantz, Anna

    2015-01-01

    Little has been done to study microbial enhanced oil recovery (MEOR) in chalk reservoirs. The present study focuses on core flooding experiments designed to see microbial plugging and its effect on oil recovery. A pressure tapped core holder was used for this purpose. A spore forming bacteria...... Bacillus licheniformis 421 was used as it was shown to be a good candidate in a previous study. Bacterial spore can penetrate deeper into the chalk rock, squeezing through the pore throats. Our results showed that injection of B. licheniformis 421 as a tertiary oil recovery method, in the residual oil...... saturation state, was able to produce additionally 1.0-2.3% original oil in place (OOIP) in homogeneous cores and 6.9-8.8% OOIP in heterogeneous cores. In addition, the pressure gradient was much higher in the heterogeneous cores, which confirms that bacterial selective plugging plays an important role...

  7. Investigation of In vitro Mineral forming bacterial isolates from supragingival calculus.

    Science.gov (United States)

    Baris, O; Demir, T; Gulluce, M

    2017-12-01

    Although it is known that bacterial mechanisms are involved in dental calculus formation, which is a predisposing factor in periodontal diseases, there have been few studies of such associations, and therefore, information available is limited. The purpose of this study was to isolate and identify aerobic bacteria responsible for direct calcification from supragingival calculus samples. The study was conducted using supragingival calculus samples from patients with periodontal disease, which was required as part of conventional treatment. Isolations were performed by sampling the supragingival calculus with buffer and inoculating the samples on media on which crystallization could be observed. The 16S recombinant DNA of the obtained pure cultures was then amplified and sequenced. A few bacterial species that have not previously been associated with mineralization or identified on bacterial plaque or calculus were detected. The bacteria that caused mineralization an aerobic environment are identified as Neisseria flava, Aggregatibacter segnis, Streptococcus tigurinus, and Morococcus cerebrosus. These findings proved that bacteria potentially play a role in the etiopathology of supragingival calculus. The association between the effects of the identified bacteria on periodontal diseases and calculus formation requires further studies.

  8. Exploiting rRNA operon copy number to investigate bacterial reproductive strategies.

    Science.gov (United States)

    Roller, Benjamin R K; Stoddard, Steven F; Schmidt, Thomas M

    2016-09-12

    The potential for rapid reproduction is a hallmark of microbial life, but microbes in nature must also survive and compete when growth is constrained by resource availability. Successful reproduction requires different strategies when resources are scarce and when they are abundant 1,2 , but a systematic framework for predicting these reproductive strategies in bacteria has not been available. Here, we show that the number of ribosomal RNA operons (rrn) in bacterial genomes predicts two important components of reproduction-growth rate and growth efficiency-which are favoured under contrasting regimes of resource availability 3,4 . We find that the maximum reproductive rate of bacteria doubles with a doubling of rrn copy number, and the efficiency of carbon use is inversely related to maximal growth rate and rrn copy number. We also identify a feasible explanation for these patterns: the rate and yield of protein synthesis mirror the overall pattern in maximum growth rate and growth efficiency. Furthermore, comparative analysis of genomes from 1,167 bacterial species reveals that rrn copy number predicts traits associated with resource availability, including chemotaxis and genome streamlining. Genome-wide patterns of orthologous gene content covary with rrn copy number, suggesting convergent evolution in response to resource availability. Our findings imply that basic cellular processes adapt in contrasting ways to long-term differences in resource availability. They also establish a basis for predicting changes in bacterial community composition in response to resource perturbations using rrn copy number measurements 5 or inferences 6,7 .

  9. Organic carbon source and salinity shape sediment bacterial composition in two China marginal seas and their major tributaries.

    Science.gov (United States)

    Wang, Kai; Zou, Li; Lu, Xinxin; Mou, Xiaozhen

    2018-08-15

    Marginal sea sediments receive organic substrates of different origins, but whether and to what extent sediment microbial communities are reflective of the different sources of organic substrates remain unclear. To address these questions, sediment samples were collected in two connected China marginal seas, i.e., Bohai Sea and Yellow Sea, and their two major tributaries (Yellow River and Liao River). Sediment bacterial community composition (BCC) was examined using 16S rRNA gene pyrosequencing. In addition, physicochemical variables that describe environmental conditions and sediment features were measured. Our results revealed that BCCs changed with salinity and organic carbon (OC) content. Members of Gaiellaceae and Comamonadaceae showed a rapid decrease as salinity and phytoplankton-derived OC increased, while Piscirickettsiaceae and Desulfobulbaceae exhibited an opposite distribution pattern. Differences of riverine vs. marginal sea sediment BCCs could be mostly explained by salinity. However, within the marginal seas, sediment BCC variations were mainly explained by OC-related variables, including terrestrial-derived fatty acids (Terr_FA), phytoplankton-derived polyunsaturated fatty acids (Phyto_PUFA), stable carbon isotopes (δ 13 C), and carbon to nitrogen ratio (C/N). In addition to environmental variables, network analysis suggested that interactions among individual bacterial taxa might be important in shaping sediment BCCs in the studied areas. Copyright © 2018 Elsevier B.V. All rights reserved.

  10. Ancient bacterial endosymbionts of insects: Genomes as sources of insight and springboards for inquiry.

    Science.gov (United States)

    Wernegreen, Jennifer J

    2017-09-15

    Ancient associations between insects and bacteria provide models to study intimate host-microbe interactions. Currently, a wealth of genome sequence data for long-term, obligately intracellular (primary) endosymbionts of insects reveals profound genomic consequences of this specialized bacterial lifestyle. Those consequences include severe genome reduction and extreme base compositions. This minireview highlights the utility of genome sequence data to understand how, and why, endosymbionts have been pushed to such extremes, and to illuminate the functional consequences of such extensive genome change. While the static snapshots provided by individual endosymbiont genomes are valuable, comparative analyses of multiple genomes have shed light on evolutionary mechanisms. Namely, genome comparisons have told us that selection is important in fine-tuning gene content, but at the same time, mutational pressure and genetic drift contribute to genome degradation. Examples from Blochmannia, the primary endosymbiont of the ant tribe Camponotini, illustrate the value and constraints of genome sequence data, and exemplify how genomes can serve as a springboard for further comparative and experimental inquiry. Copyright © 2017. Published by Elsevier Inc.

  11. Investigating Salmonella Eko from Various Sources in Nigeria by Whole Genome Sequencing to Identify the Source of Human Infections.

    Directory of Open Access Journals (Sweden)

    Pimlapas Leekitcharoenphon

    Full Text Available Twenty-six Salmonella enterica serovar Eko isolated from various sources in Nigeria were investigated by whole genome sequencing to identify the source of human infections. Diversity among the isolates was observed and camel and cattle were identified as the primary reservoirs and the most likely source of the human infections.

  12. Bacteriological investigation of ground water sources in selected ...

    African Journals Online (AJOL)

    cml

    2012-06-16

    Jun 16, 2012 ... Microbial contamination of ground water sources is a common problem in all the big cities, which endangers ... include leakage of pipes, pollution from sewerage pipes ..... and Quality Control Authority, Karachi, Pakistan.

  13. Neutron sources for neutrino investigations with the lithium converter

    International Nuclear Information System (INIS)

    Lyashuk, V.I.; Lutostansky, Yu.S.

    2012-01-01

    Creation of the powerful antineutrino source with a hard spectrum is possible on the base of β - -decay of the short lived 8 Li (T 1/2 = 0.84 s) isotope formed in the reaction 7 Li(n,γ) 8 Li. The 8 Li. isotope is a prime perspective antineutrino source taking into account that neutrino cross section depends as σ ∼ E ν 2 at the considered energy. The creation of this type powerful neutrino source (neutrino factory) is possible by (n,γ)-activation of high-purified 7 Li isotope under intensive neutron flux. As a neutron source for this purpose can be used the nuclear reactors (of steady-state flux and pulsed one), neutron sources on the base of accelerators and neutron generating targets, beam-dumps of large accelerators. The capabilities and perspectives of neutron sources are considered for the purpose of creation of the neutrino factory. Different realizations of lithium antineutrino sources (lithium converter on the base of high purified 7 Li isotope) are discussed: static regime (i.e., without transport of 8 Li isotope to the detector); dynamic regime (pumping of activated lithium to a remote detector in a closed cycle); lithium converter on the base of (a) a pulse reactors and (b) constructed as tandem of an antineutrino source and accelerator with a neutron-producing target. Heavy water solution of LiOD is proposed as a substance for the lithium converter. The expressions for neutrino fluxes in the detector position are obtained

  14. Utilization of glyphosate as phosphate source: biochemistry and genetics of bacterial carbon-phosphorous lyase

    DEFF Research Database (Denmark)

    Hove-Jensen, Bjarne; Zechel, David L; Jochimsen, Bjarne

    2014-01-01

    After several decades of use of glyphosate, the active ingredient in weed killers such as Roundup, in fields, forests, and gardens, the biochemical pathway of transformation of glyphosate phosphorus to a useful phosphorus source for microorganisms has been disclosed. Glyphosate is a member of a l...

  15. Assessment of Equine Fecal Contamination: The Search for Alternative Bacterial Source-tracking Targets

    Science.gov (United States)

    16S rDNA clone libraries were evaluated for detection of fecal source-identifying bacteria from a collapsed equine manure pile. Libraries were constructed using universal eubacterial primers and Bacteroides-Prevotella group-specific primers. Eubacterial sequences indicat...

  16. Investigations of the structure and function of bacterial communities associated with Sphagnum mosses.

    Science.gov (United States)

    Opelt, Katja; Chobot, Vladimir; Hadacek, Franz; Schönmann, Susan; Eberl, Leo; Berg, Gabriele

    2007-11-01

    High acidity, low temperature and extremely low concentration of nutrients form Sphagnum bogs into extreme habitats for organisms. Little is known about the bacteria associated with living Sphagnum plantlets, especially about their function for the host. Therefore, we analysed the endo- and ectophytic bacterial populations associated with two widely distributed Sphagnum species, Sphagnum magellanicum and Sphagnum fallax, by a multiphasic approach. The screening of 1222 isolates for antagonistic activity resulted in 326 active isolates. The bacterial communities harboured a high proportion of antifungal (26%) but a low proportion of antibacterial isolates (0.4%). Members of the genus Burkholderia (38%) were found to be the most dominant group of antagonistic bacteria. The finding that a large proportion (89%) of the antagonistic bacteria produced antifungal compounds may provide an explanation for the well-known antimicrobial activity of certain Sphagnum species. The secondary metabolites of the Sphagnum species themselves were analysed by HPLC-PDA. The different spectra of detected compounds may not only explain the antifungal activity but also the species specificity of the microbial communities. The latter was analysed using cultivation-independent single-stranded conformation polymorphism (SSCP) analysis. Using Burkholderia-specific primers we found a high diversity of Burkholderia isolates in the endophytic and ectophytic habitats of Sphagnum. Furthermore, a high diversity of nitrogen-fixing bacteria was detected by using nifH-specific primers, especially inside Sphagnum mosses. In conclusion, this study provides evidence that both Sphagnum species were colonized by characteristic bacterial populations, which appear to be important for pathogen defence and nitrogen fixation.

  17. Persistence of antibiotic resistance genes and bacterial community changes in drinking water treatment system: From drinking water source to tap water.

    Science.gov (United States)

    Su, Hao-Chang; Liu, You-Sheng; Pan, Chang-Gui; Chen, Jun; He, Liang-Ying; Ying, Guang-Guo

    2018-03-01

    As emerging contaminants, antibiotic resistance genes (ARGs) have become a public concern. This study aimed to investigate the occurrence and diversity of ARGs, and variation in the composition of bacterial communities in source water, drinking water treatment plants, and tap water in the Pearl River Delta region, South China. Various ARGs were present in the different types of water. Among the 27 target ARGs, floR and sul1 dominated in source water from three large rivers in the region. Pearson correlation analysis suggested that sul1, sul2, floR, and cmlA could be potential indicators for ARGs in water samples. The total abundance of the detected ARGs in tap water was much lower than that in source water. Sand filtration and sedimentation in drinking water treatment plants could effectively remove ARGs; in contrast, granular activated carbon filtration increased the abundance of ARGs. It was found that Pseudomonas may be involved in the proliferation and dissemination of ARGs in the studied drinking water treatment system. Bacteria and ARGs were still present in tap water after treatment, though they were significantly reduced. More research is needed to optimize the water treatment process for ARG removal. Copyright © 2017 Elsevier B.V. All rights reserved.

  18. Investigating Diet as the Source of Tetrodotoxin in Pleurobranchaea maculata

    Directory of Open Access Journals (Sweden)

    Serena Khor

    2013-12-01

    Full Text Available The origin of tetrodotoxin (TTX is highly debated; researchers have postulated either an endogenous or exogenous source with the host accumulating TTX symbiotically or via food chain transmission. The aim of this study was to determine whether the grey side-gilled sea slug (Pleurobranchaea maculata could obtain TTX from a dietary source, and to attempt to identify this source through environmental surveys. Eighteen non-toxic P. maculata were maintained in aquariums and twelve were fed a TTX-containing diet. Three P. maculata were harvested after 1 h, 24 h, 17 days and 39 days and TTX concentrations in their stomach, gonad, mantle and remaining tissue/fluids determined using liquid chromatography-mass spectrometry. Tetrodotoxin was detected in all organs/tissue after 1 h with an average uptake of 32%. This decreased throughout the experiment (21%, 15% and 9%, respectively. Benthic surveys at sites with dense populations of toxic P. maculata detected very low or no TTX in other organisms. This study demonstrates that P. maculata can accumulate TTX through their diet. However, based on the absence of an identifiable TTX source in the environment, in concert with the extremely high TTX concentrations and short life spans of P. maculata, it is unlikely to be the sole TTX source for this species.

  19. Investigation of bacterial transport in the large-block test, a thermally perturbed block of Topopah Spring tuff

    Energy Technology Data Exchange (ETDEWEB)

    Chen, C.I.; Meike, A.; Chuu, Y.J.; Sawvel, A.; Lin, W.

    1999-07-01

    Transport of bacteria is investigated as part of the Large-Block Test (LBT), a thermally perturbed block of Topopah Spring tuff. Two bacterial species, Bacillus subtilis and Arthrobacter oxydans, were isolated from the Yucca Mountain Tuff. Natural mutants that can grow under the simultaneous presence of the two antibiotics, streptomycin and rifampicin, were selected from these species by laboratory procedures, cultured, and injected into the five heater boreholes of the large block hours before heating was initiated. The temperature, as measured 5 cm above one of the heater boreholes, rose slowly over a matter of months to a maximum of 142 C and to 60 C at the top and bottom of the block. Samples were collected from boreholes located approximately 5 ft below the injection points. Double-drug-resistant microbes also appeared in the heater boreholes where the temperature had been sustainably high throughout the test. The number of double-drug-resistant bacteria that were identified in the collection boreholes increased with time until the heater was deactivated. Negative indications in the collection holes after the heater was deactivated support the supposition that these bacteria were the species that were injected. An apparent homogeneous distribution among the collection boreholes suggests no pattern to the migration of bacteria through the block. The relationship between bacterial migration and the movement of water is not yet understood. These observations indicate the possibility of rapid bacterial transport in a thermally perturbed geologic setting. The implications for colloid transport need to be reviewed.

  20. Investigation of bacterial transport in the large-block test, a thermally perturbed block of Topopah Spring tuff

    International Nuclear Information System (INIS)

    Chen, C.I.; Meike, A.; Chuu, Y.J.; Sawvel, A.; Lin, W.

    1999-01-01

    Transport of bacteria is investigated as part of the Large-Block Test (LBT), a thermally perturbed block of Topopah Spring tuff. Two bacterial species, Bacillus subtilis and Arthrobacter oxydans, were isolated from the Yucca Mountain Tuff. Natural mutants that can grow under the simultaneous presence of the two antibiotics, streptomycin and rifampicin, were selected from these species by laboratory procedures, cultured, and injected into the five heater boreholes of the large block hours before heating was initiated. The temperature, as measured 5 cm above one of the heater boreholes, rose slowly over a matter of months to a maximum of 142 C and to 60 C at the top and bottom of the block. Samples were collected from boreholes located approximately 5 ft below the injection points. Double-drug-resistant microbes also appeared in the heater boreholes where the temperature had been sustainably high throughout the test. The number of double-drug-resistant bacteria that were identified in the collection boreholes increased with time until the heater was deactivated. Negative indications in the collection holes after the heater was deactivated support the supposition that these bacteria were the species that were injected. An apparent homogeneous distribution among the collection boreholes suggests no pattern to the migration of bacteria through the block. The relationship between bacterial migration and the movement of water is not yet understood. These observations indicate the possibility of rapid bacterial transport in a thermally perturbed geologic setting. The implications for colloid transport need to be reviewed

  1. Investigation of Endophytic Bacterial Community in Supposedly Axenic Cultures of Pineapple and Orchids with Evidence on Abundant Intracellular Bacteria.

    Science.gov (United States)

    Esposito-Polesi, Natalia Pimentel; de Abreu-Tarazi, Monita Fiori; de Almeida, Cristina Vieira; Tsai, Siu Mui; de Almeida, Marcílio

    2017-01-01

    Asepsis, defined as the absence of microbial contamination, is one of the most important requirements of plant micropropagation. In long-term micropropagated cultures, there may occasionally occur scattered microorganism growth in the culture medium. These microorganisms are common plant components and are known as latent endophytes. Thus, the aim of this research was to investigate the presence of endophytic bacteria in asymptomatic pineapple and orchid microplants, which were cultivated in three laboratories for 1 year. Isolation and characterization of bacterial isolates, PCR-DGGE from total genomic DNA of microplants and ultrastructural analysis of leaves were performed. In the culture-dependent technique, it was only possible to obtain bacterial isolates from pineapple microplants. In this case, the bacteria genera identified in the isolation technique were Bacillus, Acinetobacter, and Methylobacterium. The scanning electron microscopy and transmission electron microscopy (SEM and TEM) analyses revealed the presence of endophytic bacteria in intracellular spaces in the leaves of pineapple and orchid microplants, independent of the laboratory or cultivation protocol. Our results strongly indicate that there are endophytic bacterial communities inhabiting the microplants before initiation of the in vitro culture and that some of these endophytes persist in their latent form and can also grow in the culture medium even after long-term micropropagation, thus discarding the concept of "truly axenic plants."

  2. A study investigating sound sources and noise levels in neonatal ...

    African Journals Online (AJOL)

    Background. Exposure to noise in the neonatal intensive care unit (NICU) has the potential to affect neonatal auditory development, sleep patterns and physiological stability, thus impacting on developmental progress. Objectives. This study aimed to identify noise sources in three NICUs in Johannesburg, South Africa, and ...

  3. Cultivation-independent comprehensive investigations on bacterial communities in serofluid dish, a traditional Chinese fermented food

    Directory of Open Access Journals (Sweden)

    Peng Chen

    2016-03-01

    Full Text Available Serofluid dish (or Jiangshui, in Chinese, a traditional food in the Chinese culture, is made from vegetables by fermentation. In this study, bacterial community of the fermented serofluid dish was assessed by Illumina amplicon sequencing. The metagenome comprised of 49,589 average raw reads with an average 11,497,917 bp and G+C content is 52.46%. This is the first report on V4 hyper-variable region of the 16S rRNA metagenome sequence employing Illumina platform to profile the microbial community of this little known fermented food from Gansu Province, China. The metagenome sequence can be accessed at NCBI, SRA database accession no. SRP065370. Keywords: Serofluid dish, Jiangshui, 16S rRNA, Cultivation-independent, Microbial diversity

  4. Humidity-dependent bacterial cells functional morphometry investigations using atomic force microscope.

    Science.gov (United States)

    Nikiyan, Hike; Vasilchenko, Alexey; Deryabin, Dmitry

    2010-01-01

    The effect of a relative humidity (RH) in a range of 93-65% on morphological and elastic properties of Bacillus cereus and Escherichia coli cells was evaluated using atomic force microscopy. It is shown that gradual dehumidification of bacteria environment has no significant effect on cell dimensional features and considerably decreases them only at 65% RH. The increasing of the bacteria cell wall roughness and elasticity occurs at the same time. Observed changes indicate that morphological properties of B. cereus are rather stable in wide range of relative humidity, whereas E. coli are more sensitive to drying, significantly increasing roughness and stiffness parameters at RH bacterial cells.

  5. Humidity-Dependent Bacterial Cells Functional Morphometry Investigations Using Atomic Force Microscope

    Directory of Open Access Journals (Sweden)

    Hike Nikiyan

    2010-01-01

    Full Text Available The effect of a relative humidity (RH in a range of 93–65% on morphological and elastic properties of Bacillus cereus and Escherichia coli cells was evaluated using atomic force microscopy. It is shown that gradual dehumidification of bacteria environment has no significant effect on cell dimensional features and considerably decreases them only at 65% RH. The increasing of the bacteria cell wall roughness and elasticity occurs at the same time. Observed changes indicate that morphological properties of B. cereus are rather stable in wide range of relative humidity, whereas E. coli are more sensitive to drying, significantly increasing roughness and stiffness parameters at RH ≤ 84% RH. It is discussed the dependence of the response features on differences in cell wall structure of gram-positive and gram-negative bacterial cells.

  6. Molecular hydrogen: an energy source for bacterial activity in nuclear waste disposal

    International Nuclear Information System (INIS)

    Libert, M.; Esnault, L.

    2010-01-01

    Document available in extended abstract form only. Hydrogen is a common product of microbial metabolism, large number of bacteria are able to use it as energetic substrate in subsurface ecosystems. Moreover H 2 is known as one of the most energetic substrates for deep subsurface ecosystem. It could be produced in different ways mainly volcanic activity (basalts iron rich volcanic rocks) or natural radiolysis of water or even fermentation. The millimolar concentrations of H 2 observed in the ground waters are consistent with the activity of a large variety of hydrogen-oxidising bacteria as described in the following Table. Electron acceptors are identified as O 2 , CO 2 , NO 3 , SO 4 or Fe +++ . Aerobic, anaerobic, obligate and facultative autotrophs are included. Numerous of these bacteria are thermophilic bacteria. This bacterial activity leads to the production of methane, acetate, nitrogen, hydrogen sulphur or ferrous oxides. In anoxic environments, H 2 concentrations are governed by microbial metabolism. In most cases, H 2 producing microorganisms are thermodynamically controlled by the abundance of H 2 , and survive thanks to H 2 consumers, a metabolism called inter-species H 2 transfer. Metabolism of H 2 is catalysed by hydrogenase as cytoplasmic enzymes or membrane bound enzymes. Several situations of H 2 production will occur in nuclear waste repository: - Radiolysis of water. - Radiolysis of organic matter (such as bitumen, in case of B waste), H 2 production due to gamma radiolysis of bitumen is evaluated to 1 L H 2 /kg of bitumen /MGy. - Corrosion of metal containers (in deaerated solutions). Large amount of H 2 are predicted in some situations, and will select the development of hydrogen species. Then, aerobic hydrogen bacteria oxidising hydrogen could be found in basins containing irradiating waste, or during the oxic period of storage, denitrifying bacteria or sulfate reducing bacteria will develop near the bitumen waste. Groundwater of the Callovo

  7. Investigating bacterial populations in styrene-degrading biofilters by 16S rDNA tag pyrosequencing.

    Science.gov (United States)

    Portune, Kevin J; Pérez, M Carmen; Álvarez-Hornos, F Javier; Gabaldón, Carmen

    2015-01-01

    Microbial biofilms are essential components in the elimination of pollutants within biofilters, yet still little is known regarding the complex relationships between microbial community structure and biodegradation function within these engineered ecosystems. To further explore this relationship, 16S rDNA tag pyrosequencing was applied to samples taken at four time points from a styrene-degrading biofilter undergoing variable operating conditions. Changes in microbial structure were observed between different stages of biofilter operation, and the level of styrene concentration was revealed to be a critical factor affecting these changes. Bacterial genera Azoarcus and Pseudomonas were among the dominant classified genera in the biofilter. Canonical correspondence analysis (CCA) and correlation analysis revealed that the genera Brevundimonas, Hydrogenophaga, and Achromobacter may play important roles in styrene degradation under increasing styrene concentrations. No significant correlations (P > 0.05) could be detected between biofilter operational/functional parameters and biodiversity measurements, although biological heterogeneity within biofilms and/or technical variability within pyrosequencing may have considerably affected these results. Percentages of selected bacterial taxonomic groups detected by fluorescence in situ hybridization (FISH) were compared to results from pyrosequencing in order to assess the effectiveness and limitations of each method for identifying each microbial taxon. Comparison of results revealed discrepancies between the two methods in the detected percentages of numerous taxonomic groups. Biases and technical limitations of both FISH and pyrosequencing, such as the binding of FISH probes to non-target microbial groups and lack of classification of sequences for defined taxonomic groups from pyrosequencing, may partially explain some differences between the two methods.

  8. Preliminary investigation of processes that affect source term identification

    International Nuclear Information System (INIS)

    Wickliff, D.S.; Solomon, D.K.; Farrow, N.D.

    1991-09-01

    Solid Waste Storage Area (SWSA) 5 is known to be a significant source of contaminants, especially tritium ( 3 H), to the White Oak Creek (WOC) watershed. For example, Solomon et al. (1991) estimated the total 3 H discharge in Melton Branch (most of which originates in SWSA 5) for the 1988 water year to be 1210 Ci. A critical issue for making decisions concerning remedial actions at SWSA 5 is knowing whether the annual contaminant discharge is increasing or decreasing. Because (1) the magnitude of the annual contaminant discharge is highly correlated to the amount of annual precipitation (Solomon et al., 1991) and (2) a significant lag may exist between the time of peak contaminant release from primary sources (i.e., waste trenches) and the time of peak discharge into streams, short-term stream monitoring by itself is not sufficient for predicting future contaminant discharges. In this study we use 3 H to examine the link between contaminant release from primary waste sources and contaminant discharge into streams. By understanding and quantifying subsurface transport processes, realistic predictions of future contaminant discharge, along with an evaluation of the effectiveness of remedial action alternatives, will be possible. The objectives of this study are (1) to characterize the subsurface movement of contaminants (primarily 3 H) with an emphasis on the effects of matrix diffusion; (2) to determine the relative strength of primary vs secondary sources; and (3) to establish a methodology capable of determining whether the 3 H discharge from SWSA 5 to streams is increasing or decreasing

  9. Technical and experimental investigations of a plasma focus neutron source

    International Nuclear Information System (INIS)

    Rapp, H.K.

    The results obtained from two plasma-focus devices of different size allow to report on the technical and physical properties of such neutron flash sources. The results of some diagnostic methods used for the control of the gas discharge and for the measurement of the neutron production are included. The planning of plasma focus devices is illustrated with the aid of snow-plow calculations

  10. Hospital effluents are one of several sources of metal, antibiotic resistance genes and bacterial markers disseminated in Sub-Saharan urban rivers

    Directory of Open Access Journals (Sweden)

    Amandine Laffite

    2016-07-01

    Full Text Available Data concerning the occurrence of emerging biological contaminants such as antibiotic resistance genes (ARGs and fecal indicator bacteria (FIB in aquatic environments in Sub-Saharan African countries is limited. On the other hand, antibiotic resistance remains a worldwide problem which may pose serious potential risks to human and animal health. Consequently, there is a growing number of reports concerning the prevalence and dissemination of these contaminants into various environmental compartments. Sediments provide the opportunity to reconstruct the pollution history and evaluate impacts so this study investigates the abundance and distribution of toxic metals, FIB, and ARGs released from hospital effluent wastewaters and their presence in river sediments receiving systems. ARGs (blaTEM, blaCTX-M, blaSHV, and aadA, total bacterial load, and selected bacterial species FIB (E. coli, Enterococcus (ENT and Pseudomonas species (Psd were quantified by targeting species specific genes using quantitative PCR (qPCR in total DNA extracted from the sediments recovered from 4 hospital outlet pipes (HOP and their river receiving systems in the City of Kinshasa in the Democratic Republic of the Congo. The results highlight the great concentration of toxic metals in HOP, reaching the values (in mg kg-1 of 47.9 (Cr, 213.6 (Cu, 1434.4 (Zn, 2.6 (Cd, 281.5 (Pb, and 13.6 (Hg. The results also highlight the highest (P˂0.05 values of 16S rRNA, FIB, and ARGs copy numbers in all sampling sites including upstream (control site, discharge point, and downstream of receiving rivers, indicating that the hospital effluent water is not an exclusive source of the biological contaminants entering the urban rivers. Significant correlation were observed between (i all analyzed ARGs and total bacterial load (16S rRNA 0.51 to 0.72 (p<0.001, n=65; (ii ARGs (except blaTEM and FIB and Psd 0.57 < r < 0.82 (p<0.001, n=65; and (iii ARGs (except blaTEM and toxic metals (Cd, Cr, Cu

  11. Host-Derived Sialic Acids Are an Important Nutrient Source Required for Optimal Bacterial Fitness In Vivo

    Directory of Open Access Journals (Sweden)

    Nathan D. McDonald

    2016-04-01

    Full Text Available A major challenge facing bacterial intestinal pathogens is competition for nutrient sources with the host microbiota. Vibrio cholerae is an intestinal pathogen that causes cholera, which affects millions each year; however, our knowledge of its nutritional requirements in the intestinal milieu is limited. In this study, we demonstrated that V. cholerae can grow efficiently on intestinal mucus and its component sialic acids and that a tripartite ATP-independent periplasmic SiaPQM strain, transporter-deficient mutant NC1777, was attenuated for colonization using a streptomycin-pretreated adult mouse model. In in vivo competition assays, NC1777 was significantly outcompeted for up to 3 days postinfection. NC1777 was also significantly outcompeted in in vitro competition assays in M9 minimal medium supplemented with intestinal mucus, indicating that sialic acid uptake is essential for fitness. Phylogenetic analyses demonstrated that the ability to utilize sialic acid was distributed among 452 bacterial species from eight phyla. The majority of species belonged to four phyla, Actinobacteria (members of Actinobacillus, Corynebacterium, Mycoplasma, and Streptomyces, Bacteroidetes (mainly Bacteroides, Capnocytophaga, and Prevotella, Firmicutes (members of Streptococcus, Staphylococcus, Clostridium, and Lactobacillus, and Proteobacteria (including Escherichia, Shigella, Salmonella, Citrobacter, Haemophilus, Klebsiella, Pasteurella, Photobacterium, Vibrio, and Yersinia species, mostly commensals and/or pathogens. Overall, our data demonstrate that the ability to take up host-derived sugars and sialic acid specifically allows V. cholerae a competitive advantage in intestinal colonization and that this is a trait that is sporadic in its occurrence and phylogenetic distribution and ancestral in some genera but horizontally acquired in others.

  12. COMPASS: A source term code for investigating capillary barrier performance

    International Nuclear Information System (INIS)

    Zhou, Wei; Apted, J.J.

    1996-01-01

    A computer code COMPASS based on compartment model approach is developed to calculate the near-field source term of the High-Level-Waste repository under unsaturated conditions. COMPASS is applied to evaluate the expected performance of Richard's (capillary) barriers as backfills to divert infiltrating groundwater at Yucca Mountain. Comparing the release rates of four typical nuclides with and without the Richard's barrier, it is shown that the Richard's barrier significantly decreases the peak release rates from the Engineered-Barrier-System (EBS) into the host rock

  13. Practical Investigation for Road Lighting using Renewable Energy Sources

    Directory of Open Access Journals (Sweden)

    Maged A. Abu Adma

    2017-12-01

    Full Text Available Abstract - Hybrid renewable energy systems are recently used to counteract the limitations of solar and wind as solo renewable energy sources due to adverse weather conditions. This study explains a design of a fully independent -off grid- hybrid solar and wind road lighting system according to geography and weather conditions recorded from the National Research Institute of Astronomy and Geophysics. The computerized model is designed step by step by the aid of Simulink-Matlab and the simulation was successfully run to show the performance of each module.

  14. Molecular hydrogen: An abundant energy source for bacterial activity in nuclear waste repositories

    Science.gov (United States)

    Libert, M.; Bildstein, O.; Esnault, L.; Jullien, M.; Sellier, R.

    A thorough understanding of the energy sources used by microbial systems in the deep terrestrial subsurface is essential since the extreme conditions for life in deep biospheres may serve as a model for possible life in a nuclear waste repository. In this respect, H 2 is known as one of the most energetic substrates for deep terrestrial subsurface environments. This hydrogen is produced from abiotic and biotic processes but its concentration in natural systems is usually maintained at very low levels due to hydrogen-consuming bacteria. A significant amount of H 2 gas will be produced within deep nuclear waste repositories, essentially from the corrosion of metallic components. This will consequently improve the conditions for microbial activity in this specific environment. This paper discusses different study cases with experimental results to illustrate the fact that microorganisms are able to use hydrogen for redox processes (reduction of O 2, NO3-, Fe III) in several waste disposal conditions. Consequences of microbial activity include: alteration of groundwater chemistry and shift in geochemical equilibria, gas production or consumption, biocorrosion, and potential modifications of confinement properties. In order to quantify the impact of hydrogen bacteria, the next step will be to determine the kinetic rate of the reactions in realistic conditions.

  15. Molecular hydrogen: An abundant energy source for bacterial activity in nuclear waste repositories

    International Nuclear Information System (INIS)

    Libert, M.; Bildstein, O.; Esnault, L.; Jullien, M.; Sellier, R.

    2011-01-01

    A thorough understanding of the energy sources used by microbial systems in the deep terrestrial subsurface is essential since the extreme conditions for life in deep biospheres may serve as a model for possible life in a nuclear waste repository. In this respect, H 2 is known as one of the most energetic substrates for deep terrestrial subsurface environments. This hydrogen is produced from abiotic and biotic processes but its concentration in natural systems is usually maintained at very low levels due to hydrogen-consuming bacteria. A significant amount of H 2 gas will be produced within deep nuclear waste repositories, essentially from the corrosion of metallic components. This will consequently improve the conditions for microbial activity in this specific environment. This paper discusses different study cases with experimental results to illustrate the fact that microorganisms are able to use hydrogen for redox processes (reduction of O 2 , NO 3- , Fe III) in several waste disposal conditions. Consequences of microbial activity include: alteration of groundwater chemistry and shift in geochemical equilibria, gas production or consumption, bio-corrosion, and potential modifications of confinement properties. In order to quantify the impact of hydrogen bacteria, the next step will be to determine the kinetic rate of the reactions in realistic conditions. (authors)

  16. Investigation of radiofrequency plasma sources for space travel

    International Nuclear Information System (INIS)

    Charles, C; Boswell, R W; Takahashi, K

    2012-01-01

    Optimization of radiofrequency (RF) plasma sources for the development of space thrusters differs from other applications such as plasma processing of materials since power efficiency, propellant usage, particle acceleration or heating become driving parameters. The development of two RF (13.56 MHz) plasma sources, the high-pressure (∼1 Torr) capacitively coupled ‘pocket rocket’ plasma micro-thruster and the low-pressure (∼1 mTorr) inductively coupled helicon double layer thruster (HDLT), is discussed within the context of mature and emerging electric propulsion devices. The density gradient in low-pressure expanding RF plasmas creates an electric field that accelerates positive ions out of the plasma. Generally, the total potential drop is similar to that of a wall sheath allowing the plasma electrons to neutralize the ion beam. A high-pressure expansion with no applied magnetic field can result in large dissociation rates and/or a collimated beam of ions of small area and a flowing heated neutral beam (‘pocket rocket’). A low-pressure expansion dominated by a magnetic field can result in the formation of electric double layers which produce a very directed neutralized beam of ions of large area (HDLT). (paper)

  17. Investigation of radiofrequency plasma sources for space travel

    Science.gov (United States)

    Charles, C.; Boswell, R. W.; Takahashi, K.

    2012-12-01

    Optimization of radiofrequency (RF) plasma sources for the development of space thrusters differs from other applications such as plasma processing of materials since power efficiency, propellant usage, particle acceleration or heating become driving parameters. The development of two RF (13.56 MHz) plasma sources, the high-pressure (˜1 Torr) capacitively coupled ‘pocket rocket’ plasma micro-thruster and the low-pressure (˜1 mTorr) inductively coupled helicon double layer thruster (HDLT), is discussed within the context of mature and emerging electric propulsion devices. The density gradient in low-pressure expanding RF plasmas creates an electric field that accelerates positive ions out of the plasma. Generally, the total potential drop is similar to that of a wall sheath allowing the plasma electrons to neutralize the ion beam. A high-pressure expansion with no applied magnetic field can result in large dissociation rates and/or a collimated beam of ions of small area and a flowing heated neutral beam (‘pocket rocket’). A low-pressure expansion dominated by a magnetic field can result in the formation of electric double layers which produce a very directed neutralized beam of ions of large area (HDLT).

  18. Experimental investigation of jojoba as a renewable energy source

    International Nuclear Information System (INIS)

    Al-Widyan, Mohamad I.; Al-Muhtaseb, Mu'taz A.

    2010-01-01

    This work examined jojoba (oil and cake) as possible alternative fuel sources. Jojoba is a shrub that grows very well in deserts and its cake is the solid part produced upon processing of the jojoba seeds for oil extraction. In this study, pure jojoba oil and 50/50 blends with diesel fuel were tested as fuels in a single cylinder diesel engine. The diesel fuel was the baseline of comparison throughout the runs. The cake was tested for both direct combustion (pellets) and as a substrate for biogas production. The pellets were formed by compacting the cake in cylindrical dies using a hydraulic press while the biogasification was conducted in an anaerobic digester model Bioflo 110 from Brunswick. The findings indicate that the optimum injection pressure for jojoba oil in the engine is 210 bars at which engine speed was maximum (2700 rpm), NO x and CO emissions as well as exhaust temperature were minimum. Using jojoba cake for biogasification resulted in a yield of about 600 ml biogas per 400 g of jojoba cake. Burning jojoba compacted cake (pellets) for direct combustion in a stove indicated that jojoba cake sustained a temperature in excess of 300 deg. C for a reasonable amount of time and that the cake was very competitive to wood and has energy content more than most types of wood. Overall, it may be stated that both jojoba oil and cake hold real promise as alternative energy sources.

  19. Experimental investigation of jojoba as a renewable energy source

    Energy Technology Data Exchange (ETDEWEB)

    Al-Widyan, Mohamad I. [Mechanical Engineering Department, Jordan University of Science and Technology, PO Box 3030, Irbid 22110 (Jordan); Al-Muhtaseb, Mu' taz A. [Mechanical Engineering Department, Faculty of Engineering, University of Jordan, PO Box 961060, Amman 11196 (Jordan)

    2010-08-15

    This work examined jojoba (oil and cake) as possible alternative fuel sources. Jojoba is a shrub that grows very well in deserts and its cake is the solid part produced upon processing of the jojoba seeds for oil extraction. In this study, pure jojoba oil and 50/50 blends with diesel fuel were tested as fuels in a single cylinder diesel engine. The diesel fuel was the baseline of comparison throughout the runs. The cake was tested for both direct combustion (pellets) and as a substrate for biogas production. The pellets were formed by compacting the cake in cylindrical dies using a hydraulic press while the biogasification was conducted in an anaerobic digester model Bioflo 110 from Brunswick. The findings indicate that the optimum injection pressure for jojoba oil in the engine is 210 bars at which engine speed was maximum (2700 rpm), NO{sub x} and CO emissions as well as exhaust temperature were minimum. Using jojoba cake for biogasification resulted in a yield of about 600 ml biogas per 400 g of jojoba cake. Burning jojoba compacted cake (pellets) for direct combustion in a stove indicated that jojoba cake sustained a temperature in excess of 300 C for a reasonable amount of time and that the cake was very competitive to wood and has energy content more than most types of wood. Overall, it may be stated that both jojoba oil and cake hold real promise as alternative energy sources. (author)

  20. [Investigation of bacterial and viral etiology in community acquired central nervous system infections with molecular methods].

    Science.gov (United States)

    Kahraman, Hasip; Tünger, Alper; Şenol, Şebnem; Gazi, Hörü; Avcı, Meltem; Örmen, Bahar; Türker, Nesrin; Atalay, Sabri; Köse, Şükran; Ulusoy, Sercan; Işıkgöz Taşbakan, Meltem; Sipahi, Oğuz Reşat; Yamazhan, Tansu; Gülay, Zeynep; Alp Çavuş, Sema; Pullukçu, Hüsnü

    2017-07-01

    In this multicenter prospective cohort study, it was aimed to evaluate the bacterial and viral etiology in community-acquired central nervous system infections by standart bacteriological culture and multiplex polymerase chain reaction (PCR) methods. Patients hospitalized with central nervous system infections between April 2012 and February 2014 were enrolled in the study. Demographic and clinical information of the patients were collected prospectively. Cerebrospinal fluid (CSF) samples of the patients were examined by standart bacteriological culture methods, bacterial multiplex PCR (Seeplex meningitis-B ACE Detection (Streptococcus pneumoniae, Neisseria meningitidis, Haemophilus influenzae, Listeria monocytogenes, Group B streptococci) and viral multiplex PCR (Seeplex meningitis-V1 ACE Detection kits herpes simplex virus-1 (HSV1), herpes simplex virus-2 (HSV2), varicella zoster virus (VZV), cytomegalovirus (CMV), Epstein Barr virus (EBV) and human herpes virus 6 (HHV6)) (Seeplex meningitis-V2 ACE Detection kit (enteroviruses)). Patients were classified as purulent meningitis, aseptic meningitis and encephalitis according to their clinical, CSF (leukocyte level, predominant cell type, protein and glucose (blood/CSF) levels) and cranial imaging results. Patients who were infected with a pathogen other than the detection of the kit or diagnosed as chronic meningitis and other diseases during the follow up, were excluded from the study. A total of 79 patients (28 female, 51 male, aged 42.1 ± 18.5) fulfilled the study inclusion criteria. A total of 46 patients were classified in purulent meningitis group whereas 33 were in aseptic meningitis/encephalitis group. Pathogens were detected by multiplex PCR in 41 patients. CSF cultures were positive in 10 (21.7%) patients (nine S.pneumoniae, one H.influenzae) and PCR were positive for 27 (58.6%) patients in purulent meningitis group. In this group one type of bacteria were detected in 18 patients (14 S.pneumoniae, two N

  1. The Water Cycle, a Potential Source of the Bacterial Pathogen Bacillus cereus

    Directory of Open Access Journals (Sweden)

    Julien Brillard

    2015-01-01

    Full Text Available The behaviour of the sporulating soil-dwelling Bacillus cereus sensu lato (B. cereus sl which includes foodborne pathogenic strains has been extensively studied in relation to its various animal hosts. The aim of this environmental study was to investigate the water compartments (rain and soil water, as well as groundwater closely linked to the primary B. cereus sl reservoir, for which available data are limited. B. cereus sl was present, primarily as spores, in all of the tested compartments of an agricultural site, including water from rain to groundwater through soil. During rain events, leachates collected after transfer through the soil eventually reached the groundwater and were loaded with B. cereus sl. In groundwater samples, newly introduced spores of a B. cereus model strain were able to germinate, and vegetative cells arising from this event were detected for up to 50 days. This first B. cereus sl investigation in the various types of interrelated environments suggests that the consideration of the aquatic compartment linked to soil and to climatic events should provide a better understanding of B. cereus sl ecology and thus be relevant for a more accurate risk assessment of food poisoning caused by B. cereus sl pathogenic strains.

  2. Investigation of runoff generation from anthropogenic sources with dissolved xenobiotics

    Science.gov (United States)

    Krein, A.; Pailler, J.; Guignard, C.; Iffly, J.; Pfister, L.; Hoffmann, L.

    2009-04-01

    In the experimental Mess basin (35 km2, Luxembourg) dissolved xenobiotics in surface water are used to study the influences of anthropogenic sources like separated sewer systems on runoff generation. Emerging contaminants like pharmaceuticals are of growing interest because of their use in large quantities in human and veterinary medicine. The amounts reaching surface waters depend on rainfall patterns, hydraulic conditions, consumption, metabolism, degradation, and disposal. The behaviour of endocrine disruptors including pharmaceuticals in the aquatic environment is widely unknown. The twelve molecules analyzed belong to three families: the estrogens, the antibiotics (sulfonamides, tetracyclines), and the painkillers (ibuprofen, diclofenac). Xenobiotics can be used as potential environmental tracers for untreated sewerage. Our results show that the concentrations are highly variable during flood events. The highest concentrations are reached in the first flush period, mainly during the rising limb of the flood hydrographs. As a result of the kinematic wave effect the concentration peak occurs in some cases a few hours after the discharge maximum. In floodwater (eleven floods, 66 samples) the highest concentrations were measured for ibuprofen (g/l range), estrone, and diclofenac (all ng/l range). From the tetracycline group, essentially tetracycline itself is of relevance, while the sulfonamides are mainly represented by sulfamethoxazole (all in ng/l range). In the Mess River the pharmaceuticals fluxes during flood events proved to be influenced by hydrological conditions. Different pharmaceuticals showed their concentration peaks during different times of a flood event. An example is the estrone peak that - during summer flash floods - often occurred one to two hours prior to the largest concentrations of the painkillers. This suggests for more sources than the sole storm drainage through the spillway of the single sewage water treatment plant, different

  3. Investigating Salmonella Eko from Various Sources in Nigeria by Whole Genome Sequencing to Identify the Source of Human Infections

    DEFF Research Database (Denmark)

    Leekitcharoenphon, Pimlapas; Raufu, Ibrahim; Thorup Nielsen, Mette

    2016-01-01

    Twenty-six Salmonella enterica serovar Eko isolated from various sources in Nigeria were investigated by whole genome sequencing to identify the source of human infections. Diversity among the isolates was observed and camel and cattle were identified as the primary reservoirs and the most likely...

  4. Investigation of a large volume negative hydrogen ion source

    International Nuclear Information System (INIS)

    Courteille, C.; Bruneteau, A.M.; Bacal, M.

    1995-01-01

    The electron and negative ion densities and temperatures are reported for a large volume hybrid multicusp negative ion source. Based on the scaling laws an analysis is made of the plasma formation and loss processes. It is shown that the positive ions are predominantly lost to the walls, although the observed scaling law is n + ∝I 0.57 d . However, the total plasma loss scales linearly with the discharge current, in agreement with the theoretical model. The negative ion formation and loss is also discussed. It is shown that at low pressure (1 mTorr) the negative ion wall loss becomes a significant part of the total loss. The dependence of n - /n e versus the electron temperature is reported. When the negative ion wall loss is negligible, all the data on n - /n e versus the electron temperatures fit a single curve. copyright 1995 American Institute of Physics

  5. Experimental investigation of statistical density function of decaying radioactive sources

    International Nuclear Information System (INIS)

    Salma, I.; Zemplen-Papp, E.

    1991-01-01

    The validity of the Poisson and the λ P(k) modified Poisson statistical density functions of observing k events in a short time interval is investigated experimentally in radioactive decay detection for various measuring times. The experiments to measure radioactive decay were performed with 89m Y, using a multichannel analyzer. According to the results, Poisson statistics adequately describes the counting experiment for short measuring times. (author) 13 refs.; 4 figs

  6. Glycerol as a Cheaper Carbon Source in Bacterial Cellulose (BC) Production by Gluconacetobacter Xylinus DSM46604 in Batch Fermentation System

    International Nuclear Information System (INIS)

    Azila Adnan; Nair, G.R.; Roslan Umar; Roslan Umar

    2015-01-01

    Bacterial cellulose (BC) is a polymer of glucose monomers, which has unique properties including high crystallinity and high strength. It has potential to be used in biomedical applications such as making artificial blood vessel, wound dressings, and in the paper making industry. Extensive study on BC aimed to improve BC production such as by using glycerol as a cheaper carbon source. BC was produced in shake flask culture using five different concentrations of glycerol (10, 20, 30, 40 and 50 g/ L). Using concentration of glycerol above 20 g/ L inhibited culture growth and BC production. Further experiments were performed in batch culture (3-L bioreactor) using 20 g/ L glycerol. It produced yield and productivity of 0.15 g/ g and 0.29 g/ L/ day BC, respectively. This is compared with the control medium, 50 g/ L glucose, which only gave yield and productivity of 0.05 g/ g and 0.23 g/ L/ day, respectively. Twenty g/ L of glycerol enhanced BC production by Gluconacetobacter xylinus DSM46604 in batch fermentation system. (author)

  7. Chemical synthesis of perfectly isotactic and high melting bacterial poly(3-hydroxybutyrate) from bio-sourced racemic cyclic diolide.

    Science.gov (United States)

    Tang, Xiaoyan; Chen, Eugene Y-X

    2018-06-11

    Bacterial poly(3-hydroxybutyrate) (P3HB) is a perfectly isotactic, crystalline material possessing properties suitable for substituting petroleum plastics, but high costs and low volumes of its production are impractical for commodity applications. The chemical synthesis of P3HB via ring-opening polymerization (ROP) of racemic β-butyrolactone has attracted intensive efforts since the 1960s, but not yet produced P3HB with high isotacticity and molecular weight. Here, we report a route utilizing racemic cyclic diolide (rac-DL) derived from bio-sourced succinate. With stereoselective racemic catalysts, the ROP of rac-DL under ambient conditions produces rapidly P3HB with perfect isotacticity ([mm] > 99%), high melting temperature (T m  = 171 °C), and high molecular weight (M n  = 1.54 × 10 5  g mol -1 , Đ = 1.01). With enantiomeric catalysts, kinetic resolution polymerizations of rac-DL automatically stops at 50% conversion and yields enantiopure (R,R)-DL and (S,S)-DL with >99% e.e. and the corresponding poly[(S)-3HB] and poly[(R)-3HB] with high T m  = 175 °C.

  8. Investigation on paper cup waste degradation by bacterial consortium and Eudrillus eugeinea through vermicomposting.

    Science.gov (United States)

    Arumugam, Karthika; Renganathan, Seenivasagan; Babalola, Olubukola Oluranti; Muthunarayanan, Vasanthy

    2018-04-01

    Disposable Paper cups are a threat to the environment and are composed of 90% high strength paper with 5% thin coating of polyethylene. This polyethylene prevents the paper cup from undergoing degradation in the soil. Hence, in the present study two different approaches towards the management of paper cup waste through vermicomposting technology has been presented. The experimental setup includes 2 plastic reactors namely Vermicompost (VC) (Cow dung + Paper cup waste + Earthworm (Eudrillus eugeinea)) and Vermicompost with bacterial consortium (VCB) (Cow dung + Paper cup waste + Eudrillus eugeinea + Microbial consortia such as Bacillus anthracis, B. endophyticus, B. funiculus, B. thuringiensis, B. cereus, B. toyonensis, Virigibacillius chiquenigi, Acinetobacter baumanni and Lactobacillus pantheries). After treatment the physicochemical parameters were analysed. The results showed that the values of TOC (26.52 and 37.47%), TOM (36.01 and 33.13%) and C/N (15.02 and 11.92%) ratio are reduced in both VC and VCB whereas, the values of pH (8.01 and 7.56), EC (1.2-1.9 µs -1 and 1.4-1.9 µs -1 ), TP (46.1 and 51%), TMg (50.52 and 64.3%), TCa (50 and 64%), TNa (1.39 and 1.75%) and TK (1.75 and 1.86%) have increased. This study substantiates the addition of the microbial consortia augmenting the degradation in VCB reactor by reducing the period of process from 19 to 12 weeks. Further the characterisation of the vermicompost prepared from paper cup with FT-IR shows high degradation of carboxylic and aliphatic group; SEM analysis shows the disaggregation of cellulose and lignin; XRD shows the degradation of cellulose. All these analyses endorse the degradation of the paper cup waste faster with microbes (VCB). Thus, this present study high lights management of the paper cup waste in a relatively short period of time. Copyright © 2017 Elsevier Ltd. All rights reserved.

  9. Host-Derived Sialic Acids Are an Important Nutrient Source Required for Optimal Bacterial Fitness In Vivo.

    Science.gov (United States)

    McDonald, Nathan D; Lubin, Jean-Bernard; Chowdhury, Nityananda; Boyd, E Fidelma

    2016-04-12

    A major challenge facing bacterial intestinal pathogens is competition for nutrient sources with the host microbiota.Vibrio cholerae is an intestinal pathogen that causes cholera, which affects millions each year; however, our knowledge of its nutritional requirements in the intestinal milieu is limited. In this study, we demonstrated that V. cholerae can grow efficiently on intestinal mucus and its component sialic acids and that a tripartite ATP-independent periplasmic SiaPQM strain, transporter-deficient mutant NC1777, was attenuated for colonization using a streptomycin-pretreated adult mouse model. In in vivo competition assays, NC1777 was significantly outcompeted for up to 3 days postinfection. NC1777 was also significantly outcompeted in in vitro competition assays in M9 minimal medium supplemented with intestinal mucus, indicating that sialic acid uptake is essential for fitness. Phylogenetic analyses demonstrated that the ability to utilize sialic acid was distributed among 452 bacterial species from eight phyla. The majority of species belonged to four phyla, Actinobacteria (members of Actinobacillus, Corynebacterium, Mycoplasma, and Streptomyces), Bacteroidetes (mainly Bacteroides, Capnocytophaga, and Prevotella), Firmicutes (members of Streptococcus, Staphylococcus, Clostridium, and Lactobacillus), and Proteobacteria (including Escherichia, Shigella, Salmonella, Citrobacter, Haemophilus, Klebsiella, Pasteurella, Photobacterium, Vibrio, and Yersinia species), mostly commensals and/or pathogens. Overall, our data demonstrate that the ability to take up host-derived sugars and sialic acid specifically allows V. cholerae a competitive advantage in intestinal colonization and that this is a trait that is sporadic in its occurrence and phylogenetic distribution and ancestral in some genera but horizontally acquired in others. Sialic acids are nine carbon amino sugars that are abundant on all mucous surfaces. The deadly human pathogen Vibrio cholerae contains

  10. Methods for dynamic investigations of surface-attached in vitro bacterial and fungal biofilms

    DEFF Research Database (Denmark)

    Sternberg, Claus; Bjarnsholt, Thomas; Shirtliff, Mark

    2014-01-01

    Three dynamic models for the investigation of in vitro biofilm formation are described in this chapter. In the 6-well plate assay presented here, the placing of the plate on a rotating platform provides shear, thereby making the system dynamic with respect to the static microtiter assay.The second...... reported model, especially suitable for harvesting high amounts of cells for transcriptomic or proteomic investigations, is based on numerous glass beads placed in a flask incubated with shaking on a rotating platform, thus increasing the surface area for biofilm formation. Finally, the flow-cell system...

  11. BACTERIAL SOURCE TRACKING

    Science.gov (United States)

    Fecal contamination of surface waters used for recreation, drinking water and aquaculture are a continuous environmental problem and pose significant human health risks. An alarming amount of the United States rivers/streams (39%), lakes (45%), and estuaries (51%) are not safe f...

  12. Bacterial growth on ion exchange resin - investigations with a strong cationic exchanger. Pt. 3. Disinfection with peracetic acid

    Energy Technology Data Exchange (ETDEWEB)

    Flemming, H.C.

    1984-12-01

    The suitability of peracetic acid (PAA) for the disinfection of ion exchangers was investigated. 0.02% PAA is suitable for satisfactory disinfection. In this way corrosive effects are strongly reduced. Ca/sup 2+/-ions seem to protect the bacteria, therefore the disinfection should be done with the Na/sup +/-form. The disinfection has no remanent effect and therefore is not suitable for preventing bacterial aftergrowth during off-periods. A combination of silver and disinfectant can accomplish this, until a new, silver-tolerant microflora has evolved. In this case the use of 0.02% PAA is imperative, because higher concentrations will dissolve the silver. As a principle the effectiveness of disinfection procedure should be monitored bacteriologically.

  13. Liver is the major source of elevated serum lipocalin-2 levels after bacterial infection or partial hepatectomy

    DEFF Research Database (Denmark)

    Xu, Ming-Jiang; Feng, Dechun; Wu, Hailong

    2015-01-01

    cell type responsible for LCN2 production after bacterial infection or PHx, and this response is dependent on IL-6 activation of the STAT3 signaling pathway. Thus, hepatocyte-derived LCN2 plays an important role in inhibiting bacterial infection and promoting liver regeneration....... or E. coli. These mice also had increased enteric bacterial translocation from the gut to the mesenteric lymph nodes and exhibited reduced liver regeneration after PHx. Treatment with interleukin (IL)-6 stimulated hepatocytes to produce LCN2 in vitro and in vivo. Hepatocyte-specific ablation of the IL...

  14. Intense resonance neutron source (IREN) - new pulsed source for nuclear physical and applied investigations

    International Nuclear Information System (INIS)

    Anan'ev, V.D.; Furman, W.I.; Kobets, V.V.; Meshkov, I.N.; Pyataev, V.G.; Shirkov, G.D.; Shvets, V.A.; Sumbaev, A.P.; Kuatbekov, R.P.; Tret'yakov, I.T.; Frolov, A.R.; Gurov, S.M.; Logachev, P.V.; Pavlov, V.M.; Skarbo, B.A.

    2005-01-01

    An accelerator-driven subcritical system (200 MeV electron linac + metallic plutonium subcritical core) IREN is constructed at the Joint Institute for Nuclear Research (JINR). The new pulsed neutron source IREN is optimized for maximal yield of resonance neutrons (1-10 5 eV). The S-band electron linac with a pulse duration near 200 ns, repetition rate up to 150 Hz and the mean beam power 10 kW delivers 200-MeV electrons onto a specially designed tungsten target (an electron-neutron converter) situated in the center of a very compact and fast subcritical assembly with K eff 15 per second. A mean fission power of the multiplying target is planned to be near 15 kW. The current status of the project is presented

  15. Investigations Concerning Possibilities of Diagnosis and Treatement in One Pesthole of Bacterial Haemorrhagic Septicemia in Carp (Cyprinus Carpio

    Directory of Open Access Journals (Sweden)

    Octavian Negrea

    2010-10-01

    Full Text Available Investigations were done on 7 alive samples in agonic or death stage, taken from an extensive exploitation pond, innorth-east Transylvanian zone, with an water surface of 0,2 ha and a total fish quantity of about 200 kg, secondsummer carp of an average weight of 300 g/individual. As a result of clinical, bacterioscopy and bacteriology andalso necropsy laboratory exam it were put in evidence some aspects. So, clinical exam has revealed, by the presenceof integument hemorrhagic ulcerous lesions, also the presence of some crustacaee ectoparasites, Argulus foliaceusspecies, which have made easier the disease spreading in whole effective. Necropsy exam puts in evidence, anatomyclinic,the presence of integument hemorrhagic lesions under a point or diffuse form, but also ulcers and muscle –cutaneous necrosis. As a following of bacterioscopy exam of smears done from pathologic material taken incutaneous lesions and colored by Gram method it was ascertained the presence of a bacterial polymorph flora, ofbacillus and cocobacillus type, Gram negative. Bacteriologic exam (cultural, realized on ordinary culture mediums(sauce, water and selective mediums (agar with blood isolates in pure culture germs from Aeromonas kind, basis onmorphological and cultural characters, which are framed in Aeromonas genus patterns described by specialityliterature. As a following of biochemical characters exam, with the help of multitest API20E system it wasestablished that Aeromonas spp. strains isolated, belong to hydrophila group 1 species, species which is responsibleof bacterial hemorrhagic septicemia performing in carp. By testing the sensitiveness to antibodies and chemiotherapyin strains which have been found as diagnosis (diffusiometric method of antibiogram, it was ascertained that group 1Aeromonas hydrophila strains, isolated from disease pesthole, were in decreasing order sensitive to: Eurofloxocin,Florfenicol (Floron and Oxitetracycline and resistant to

  16. Investigation of bacterial transport in the large-block test, a thermally perturbed block of Topopah Spring Tuff

    International Nuclear Information System (INIS)

    Chen, C. I.; Chuu, Y. J.; Lin, W.; Meike, A.; Sawvel, A.

    1998-01-01

    This study investigates the transport of bacteria in a large, thermally perturbed block of Topopah Spring tuff. The study was part of the Large-Block Test (LBT), thermochemical and physical studies conducted on a 10 ft x 10 ft x 14 ft block of volcanic tuff excavated on 5 of 6 sides out of Fran Ridge, Nevada. Two bacterial species, Bacillus subtilis and Arthrobacter oxydans, were isolated from the Yucca Mountain tuff. Natural mutants that can grow under the simultaneous presence of the two antibiotics, streptomycin and rifampicin, were selected from these species by laboratory procedures. The double-drug-resistant mutants, which could be thus distinguished from the indigenous species, were injected into the five heater boreholes of the large block hours before heating was initiated. The temperature, as measured 5 cm above one of the heater boreholes, rose slowly and steadily over a matter of months to a maximum of 142 C. Samples (cotton cloths inserted the length of the hole, glass fiber swabs, and filter papers) were collected from the boreholes that were approximately 5 ft below the injection points. Double-drug-resistant bacteria were found in the collection boreholes nine months after injection. Surprisingly, they also appeared in the heater boreholes where the temperature had been sustainably high throughout the test. These bacteria appear to be the species that were injected. The number of double-drug-resistant bacteria that were identified in the collection boreholes increased with time. An apparent homogeneous distribution among the observation boreholes and heater boreholes suggests that a random motion could be the pattern that the bacteria migrated in the block. These observations indicated the possibility of rapid bacterial transport in a thermally perturbed geologic setting

  17. Structural Variation in Bacterial Glyoxalase I Enzymes: Investigation of the Metalloenzyme Glyoxalase I from Clostridium acetobutylicum

    Energy Technology Data Exchange (ETDEWEB)

    Suttisansanee U.; Swaminathan S.; Lau, K.; Lagishetty, S.; Rao, K. N.; Sauder, J. M.; Burley, S. K.; Honek, J. F.

    2011-11-04

    The glyoxalase system catalyzes the conversion of toxic, metabolically produced {alpha}-ketoaldehydes, such as methylglyoxal, into their corresponding nontoxic 2-hydroxycarboxylic acids, leading to detoxification of these cellular metabolites. Previous studies on the first enzyme in the glyoxalase system, glyoxalase I (GlxI), from yeast, protozoa, animals, humans, plants, and Gram-negative bacteria, have suggested two metal activation classes, Zn{sup 2+} and non-Zn{sup 2+} activation. Here, we report a biochemical and structural investigation of the GlxI from Clostridium acetobutylicum, which is the first GlxI enzyme from Gram-positive bacteria that has been fully characterized as to its three-dimensional structure and its detailed metal specificity. It is a Ni{sup 2+}/Co{sup 2+}-activated enzyme, in which the active site geometry forms an octahedral coordination with one metal atom, two water molecules, and four metal-binding ligands, although its inactive Zn{sup 2+}-bound form possesses a trigonal bipyramidal geometry with only one water molecule liganded to the metal center. This enzyme also possesses a unique dimeric molecular structure. Unlike other small homodimeric GlxI where two active sites are located at the dimeric interface, the C. acetobutylicum dimeric GlxI enzyme also forms two active sites but each within single subunits. Interestingly, even though this enzyme possesses a different dimeric structure from previously studied GlxI, its metal activation characteristics are consistent with properties of other GlxI. These findings indicate that metal activation profiles in this class of enzyme hold true across diverse quaternary structure arrangements.

  18. Bleeding Sap and Old Wood Are the Two Main Sources of Contamination of Merging Organs of Vine Plants by Xylophilus ampelinus, the Causal Agent of Bacterial Necrosis

    OpenAIRE

    Grall, S.; Roulland, C.; Guillaumès, J.; Manceau, C.

    2005-01-01

    The spatial distribution of vine plants contaminated by Xylophilus ampelinus, the agent responsible for bacterial necrosis, was studied over a 5-year period within two vineyards in the Cognac area. Both vineyards were planted with Vitis vinifera cv. Ugni blanc but were different in age and agronomic location. The emission of X. ampelinus in contaminated bleeding sap was observed during vine sprouting. Contaminated bleeding sap is an important source of inoculum for external contamination due ...

  19. The hypertext information system on pulsed neutron sources and scientific investigations based on these sources

    Energy Technology Data Exchange (ETDEWEB)

    Litvinenko, E I; Astakhov, Yu A [Frank Laboratory of Neutron Physics, Joint Institute for Nuclear Research, Dubna (Russian Federation); Akishina, E P [Moscow State Engineering Physics Institute (Technical University), Moscow (Russian Federation); Semenov, R N; Smol` kov, I S [International University ` Dubna` , Dubna (Russian Federation)

    1998-10-01

    The work on the creation of the hypertext information system has been performed on the basis of the web-server of the Frank Laboratory of Neutron Physics, JINR. The initial project proposed the creation of HTML information resources and did not consider the usage of any database for the information management. During the project implementation it became obvious that the system should have well defined structured informational model and it might be helpful to imply the relational database as a part of the system. The ORACLE server at the Laboratory of Computing Techniques and Automation (LCTA) of the JINR has been used for this task. Now we have a set of ORACLE tables designed using CASE tools for the informational model of the system, structured information about neutron sources, neutron instruments, printed publications and URL addresses. We have also the web interface to these tables using free ware gateway ORALINK installed on our Pentium PC with Windows NT and some tools to administer database and view pictures stored in the tables. We took into account NeXuS specifications while tried to design the informational model of the system, and we continue to work on its creation 15 refs., 2 figs., 1 tab. Submitted to the Proceedings of the International Workshop on New Opportunities for Better User Group Software (NOBUGS`97), 10-12 Dec 1997, USA

  20. The hypertext information system on pulsed neutron sources and scientific investigations based on these sources

    International Nuclear Information System (INIS)

    Litvinenko, E.I; Astakhov, Yu.A.; Akishina, E.P.; Semenov, R.N.; Smol'kov, I.S.

    1998-01-01

    The work on the creation of the hypertext information system has been performed on the basis of the web-server of the Frank Laboratory of Neutron Physics, JINR. The initial project proposed the creation of HTML information resources and did not consider the usage of any database for the information management. During the project implementation it became obvious that the system should have well defined structured informational model and it might be helpful to imply the relational database as a part of the system. The ORACLE server at the Laboratory of Computing Techniques and Automation (LCTA) of the JINR has been used for this task. Now we have a set of ORACLE tables designed using CASE tools for the informational model of the system, structured information about neutron sources, neutron instruments, printed publications and URL addresses. We have also the web interface to these tables using free ware gateway ORALINK installed on our Pentium PC with Windows NT and some tools to administer database and view pictures stored in the tables. We took into account NeXuS specifications while tried to design the informational model of the system, and we continue to work on its creation

  1. Investigations of Orchestra Auralizations Using the Multi-Channel Multi-Source Auralization Technique

    DEFF Research Database (Denmark)

    Vigeant, Michelle; Wang, Lily M.; Rindel, Jens Holger

    2008-01-01

    a multi-channel multi-source auralization technique, involving individual five-channel anechoic recordings of each instrumental part of two symphonies. In the first study, these auralizations were subjectively compared to orchestra auralizations made using (a) a single omni-directional source, (b......) a surface source, and (c) single-channel multi-source method. Results show that the multi-source auralizations were rated to be more realistic than the surface source ones and to have larger source width than the single omni-directional source auralizations. No significant differences were found between......Room acoustics computer modeling is a tool for generating impulse responses and auralizations from modeled spaces. The auralizations are commonly made from a single-channel anechoic recording of solo instruments. For this investigation, auralizations of an entire orchestra were created using...

  2. Investigating the influence of DNAPL spill characteristics on source zone architecture and mass removal in pool-dominated source zones

    Science.gov (United States)

    Wallace, K. A.; Abriola, L.; Chen, M.; Ramsburg, A.; Pennell, K. D.; Christ, J.

    2009-12-01

    Multiphase, compositional simulators were employed to investigate the spill characteristics and subsurface properties that lead to pool-dominated, dense non-aqueous phase liquid (DNAPL) source zone architectures. DNAPL pools commonly form at textural interfaces where low permeability lenses restrict the vertical migration of DNAPL, allowing for DNAPL to accumulate, reaching high saturation. Significant pooling has been observed in bench-scale experiments and field settings. However, commonly employed numerical simulations rarely predict the pooling suspected in the field. Given the importance of pooling on the efficacy of mass recovery and the down-gradient contaminant signal, it is important to understand the predominant factors affecting the creation of pool-dominated source zones and their subsequent mass discharge. In this work, contaminant properties, spill characteristics and subsurface permeability were varied to investigate the factors contributing to the development of a pool-dominated source zone. DNAPL infiltration and entrapment simulations were conducted in two- and three-dimensional domains using the University of Texas Chemical Compositional (UTCHEM) simulator. A modified version of MT3DMS was then used to simulate DNAPL dissolution and mass discharge. Numerical mesh size was varied to investigate the importance of numerical model parameters on simulations results. The temporal evolution of commonly employed source zone architecture metrics, such as the maximum DNAPL saturation, first and second spatial moments, and fraction of DNAPL mass located in pools, was monitored to determine how the source zone architecture evolved with time. Mass discharge was monitored to identify the link between source zone architecture and down-gradient contaminant flux. Contaminant characteristics and the presence of extensive low permeability lenses appeared to have the most influence on the development of a pool-dominated source zone. The link between DNAPL mass

  3. Zinc source and concentration altered physiological responses of beef heifers during a combined viral-bacterial respiratory challenge

    Science.gov (United States)

    Three treatments were evaluated in feedlot heifers to determine the effects of zinc supplementation on the immune response to a combined viral-bacterial respiratory disease challenge. Thirty-two beef heifers (255+/-15 kg) were subjected to a 30d period of Zn depletion, then randomly assigned to one ...

  4. Effect of Different Carbon Sources on Bacterial Nanocellulose Production and Structure Using the Low pH Resistant Strain Komagataeibacter Medellinensis

    Directory of Open Access Journals (Sweden)

    Carlos Molina-Ramírez

    2017-06-01

    Full Text Available Bacterial cellulose (BC is a polymer obtained by fermentation with microorganism of different genera. Recently, new producer species have been discovered, which require identification of the most important variables affecting cellulose production. In this work, the influence of different carbon sources in BC production by a novel low pH-resistant strain Komagataeibacter medellinensis was established. The Hestrin-Schramm culture medium was used as a reference and was compared to other media comprising glucose, fructose, and sucrose, used as carbon sources at three concentrations (1, 2, and 3% w/v. The BC yield and dynamics of carbon consumption were determined at given fermentation times during cellulose production. While the carbon source did not influence the BC structural characteristics, different production levels were determined: glucose > sucrose > fructose. These results highlight considerations to improve BC industrial production and to establish the BC property space for applications in different fields.

  5. Bacterial indicator occurrence and the use of an F+ specific RNA coliphage assay to identify fecal sources in Homosassa Springs, Florida

    Science.gov (United States)

    Griffin, Dale W.; Stokes, Rodger; Rose, J.B.; Paul, J.H.

    2000-01-01

    A microbiological water quality study of Homosassa Springs State Wildlife Park (HSSWP) and surrounding areas was undertaken. Samples were collected in November of 1997 (seven sites) and again in November of 1998 (nine sites). Fecal bacterial concentrations (total and fecal coliforms, Clostridium perfringens, and enterococci) were measured as relative indicators of fecal contamination. F+-specific coliphage genotyping was performed to determine the source of fecal contamination at the study sites. Bacterial levels were considerably higher at most sites in the 1997 sampling compared to the 1998 sampling, probably because of the greater rainfall that year. In November of 1997, 2 of the 7 sites were in violation of all indicator standards and guidance levels. In November of 1998, 1 of 9 sites was in violation of all indicator standard and guidance levels. The highest concentrations of all fecal indicators were found at a station downstream of the animal holding pens in HSSWP. The lowest levels of indicators were found at the Homosassa Main Spring vent. Levels of fecal indicators downstream of HSSWP (near the point of confluence with the river) were equivalent to those found in the Southeastern Fork and areas upstream of the park influences. F+ specific RNA coliphage analysis indicated that fecal contamination at all sites that tested positive was from animal sources (mammals and birds). These results suggest that animal (indigenous and those in HSSWP) and not human sources influenced microbial water quality in the area of Homosassa River covered by this study.

  6. Upstream Freshwater and Terrestrial Sources Are Differentially Reflected in the Bacterial Community Structure along a Small Arctic River and Its Estuary

    DEFF Research Database (Denmark)

    Hauptmann, Aviaja Zenia Edna Lyberth; Markussen, Thor N; Stibal, Marek

    2016-01-01

    of different water sources on the microbial communities in Arctic rivers and estuaries remains unknown. In this study we used 16S rRNA gene amplicon sequencing to assess a small river and its estuary on the Disko Island, West Greenland (69°N). Samples were taken in August when there is maximum precipitation...... and temperatures are high in the Disko Bay area. We describe the bacterial community through a river into the estuary, including communities originating in a glacier and a proglacial lake. Our results show that water from the glacier and lake transports distinct communities into the river in terms of diversity...

  7. Bacterial, viral and turbidity removal by intermittent slow sand filtration for household use in developing countries: experimental investigation and modeling.

    Science.gov (United States)

    Jenkins, Marion W; Tiwari, Sangam K; Darby, Jeannie

    2011-11-15

    .5%) and mean 0.94 log removal of MS2 viruses (88.5%) were achieved. Results point to new recommendations regarding filter design, manufacture, and operation for implementing ISSFs in local settings in developing countries. Sand size emerged as a critical design factor on performance. A single layer of river sand used in this investigation demonstrated removals comparable to those reported for 2 layers of crushed sand. Pause time and increased residence time each emerged as highly beneficial for improving removal performance on all four outcomes. A relatively large and significant negative effect of influent turbidity on MS2 viral removal in the ISSF was measured in parallel with a much smaller weaker positive effect of influent turbidity on FC bacterial removal. Disturbance of the schmutzdecke by wet harrowing showed no effect on virus removal and a modest reductive effect on the bacterial and turbidity removal as measured 7 days or more after the disturbance. For existing coarse sand ISSFs, this research indicates that a reduction in batch feed volume, effectively reducing the operating head and increasing the pore:batch volume ratio, could improve their removal performance by increasing batch residence time. Copyright © 2011 Elsevier Ltd. All rights reserved.

  8. Investigating the effects of different factors on development of open source enterprise resources planning software packages

    Directory of Open Access Journals (Sweden)

    Mehdi Ghorbaninia

    2014-08-01

    Full Text Available This paper investigates the effects of different factors on development of open source enterprise resources planning software packages. The study designs a questionnaire in Likert scale and distributes it among 210 experts in the field of open source software package development. Cronbach alpha has been calculated as 0.93, which is well above the minimum acceptable level. Using Pearson correlation as well as stepwise regression analysis, the study determines three most important factors including fundamental issues, during and after implementation of open source software development. The study also determines a positive and strong relationship between fundamental factors and after implementation factors (r=0.9006, Sig. = 0.000.

  9. An investigation of the closure problem applied to reactor accident source terms

    International Nuclear Information System (INIS)

    Brearley, I.R.; Nixon, W.; Hayns, M.R.

    1987-01-01

    The closure problem, as considered here, focuses attention on the question of when in current research programmes enough has been learned about the source terms for reactor accident releases. Noting that current research is tending to reduce the estimated magnitude of the aerosol component of atmospheric, accidental releases, several possible criteria for closure are suggested. Moreover, using the reactor accident consequence model CRACUK, the effect of gradually reducing the aerosol release fractions of a pressurized water reactor (PWR2) source term (as defined in the WASH-1400 study) is investigated and the implications of applying the suggested criteria to current source term research discussed. (author)

  10. Electrical conductivity measurements of bacterial nanowires from Pseudomonas aeruginosa

    International Nuclear Information System (INIS)

    Maruthupandy, Muthusamy; Anand, Muthusamy; Beevi, Akbar Sait Hameedha; Priya, Radhakrishnan Jeeva; Maduraiveeran, Govindhan

    2015-01-01

    The extracellular appendages of bacteria (flagella) that transfer electrons to electrodes are called bacterial nanowires. This study focuses on the isolation and separation of nanowires that are attached via Pseudomonas aeruginosa bacterial culture. The size and roughness of separated nanowires were measured using transmission electron microscopy (TEM) and atomic force microscopy (AFM), respectively. The obtained bacterial nanowires indicated a clear image of bacterial nanowires measuring 16 nm in diameter. The formation of bacterial nanowires was confirmed by microscopic studies (AFM and TEM) and the conductivity nature of bacterial nanowire was investigated by electrochemical techniques. Cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS), which are nondestructive voltammetry techniques, suggest that bacterial nanowires could be the source of electrons—which may be used in various applications, for example, microbial fuel cells, biosensors, organic solar cells, and bioelectronic devices. Routine analysis of electron transfer between bacterial nanowires and the electrode was performed, providing insight into the extracellular electron transfer (EET) to the electrode. CV revealed the catalytic electron transferability of bacterial nanowires and electrodes and showed excellent redox activities. CV and EIS studies showed that bacterial nanowires can charge the surface by producing and storing sufficient electrons, behave as a capacitor, and have features consistent with EET. Finally, electrochemical studies confirmed the development of bacterial nanowires with EET. This study suggests that bacterial nanowires can be used to fabricate biomolecular sensors and nanoelectronic devices. (paper)

  11. Corn cob biochar increases soil culturable bacterial abundance without enhancing their capacities in utilizing carbon sources in Biolog Eco-plates

    Institute of Scientific and Technical Information of China (English)

    JIANG Lin-lin; HAN Guang-ming; LAN Yu; LIU Sai-nan; GAO Ji-ping; YANG Xu; MENG Jun; CHEN Wen-fu

    2017-01-01

    Biochar has been shown to influence soil microbial communities in terms of their abundance and diversity.However,the relationship among microbial abundance,structure and C metabolic traits is not well studied under biochar application.Here it was hypothesized that the addition of biochar with intrinsic properties (i.e.,porous structure) could affect the proliferation of culturable microbes and the genetic structure of soil bacterial communities.In the meantime,the presence of available organic carbon in biochar may influence the C utilization capacities of microbial community in Biolog Eco-plates.A pot experiment was conducted with differenct biochar application (BC) rates:control (0 t ha-1),BC1 (20 t ha-1) and BC2 (40 t ha-1).Culturable microorganisms were enumerated via the plate counting method.Bacterial diversity was examined using denaturing gradient gel electrophoresis (DGGE).Microbial capacity in using C sources was assessed using Biolog Eco-plates.The addition of biochar stimulated the growth of actinomyces and bacteria,especially the ammonifying bacteria and azotobacteria,but had no significant effect on fungi proliferation.The phylogenetic distribution of the operational taxonomic units could be divided into the following groups with the biochar addition:Firmicutes,Acidobacteria,Gemmatimonadetes,Actinobacteria,Cyanobacteria and α-,β-,γ-and δ-Proteobacteria (average similarity >95%).Biochar application had a higher capacity utilization for L-asparagine,Tween 80,D-mannitol,L-serine,γ-hydroxybutyric acid,N-acetyI-D-glucosamine,glycogen,itaconic acid,glycyl-L-glutamic acid,α-ketobutyricacid and putrescine,whereas it had received decreased capacities in using the other 20 carbon sources in Biolog Eco-plates.Redundancy analysis (RDA) revealed that the physico-chemical properties,indices of bacterial diversity,and C metabolic traits were positively correlated with the appearance of novel sequences under BC2 treatment.Our study indicates that the

  12. Investigation of environmental drivers of antimicrobial resistance in foodborne bacterial pathogens in antibiotic-free, all natural, pastured poultry flocks.

    Science.gov (United States)

    Question: In the absence of antibiotic use within pastured poultry production, what are potential environmental variables that drive the antimicrobial sensitivity patterns of bacterial foodborne pathogens isolated from these flocks? Purpose: The objective of this study is to examine environmental f...

  13. An international point source outbreak of typhoid fever: a European collaborative investigation*

    Science.gov (United States)

    Stanwell-Smith, R. E.; Ward, L. R.

    1986-01-01

    A point source outbreak of Salmonella typhi, degraded Vi-strain 22, affecting 32 British visitors to Kos, Greece, in 1983 was attributed by a case—control study to the consumption of a salad at one hotel. This represents the first major outbreak of typhoid fever in which a salad has been identified as the vehicle. The source of the infection was probably a carrier in the hotel staff. The investigation demonstrates the importance of national surveillance, international cooperation, and epidemiological methods in the investigation and control of major outbreaks of infection. PMID:3488842

  14. Next-generation sequencing identification of pathogenic bacterial genes and their relationship with fecal indicator bacteria in different water sources in the Kathmandu Valley, Nepal.

    Science.gov (United States)

    Ghaju Shrestha, Rajani; Tanaka, Yasuhiro; Malla, Bikash; Bhandari, Dinesh; Tandukar, Sarmila; Inoue, Daisuke; Sei, Kazunari; Sherchand, Jeevan B; Haramoto, Eiji

    2017-12-01

    Bacteriological analysis of drinking water leads to detection of only conventional fecal indicator bacteria. This study aimed to explore and characterize bacterial diversity, to understand the extent of pathogenic bacterial contamination, and to examine the relationship between pathogenic bacteria and fecal indicator bacteria in different water sources in the Kathmandu Valley, Nepal. Sixteen water samples were collected from shallow dug wells (n=12), a deep tube well (n=1), a spring (n=1), and rivers (n=2) in September 2014 for 16S rRNA gene next-generation sequencing. A total of 525 genera were identified, of which 81 genera were classified as possible pathogenic bacteria. Acinetobacter, Arcobacter, and Clostridium were detected with a relatively higher abundance (>0.1% of total bacterial genes) in 16, 13, and 5 of the 16 samples, respectively, and the highest abundance ratio of Acinetobacter (85.14%) was obtained in the deep tube well sample. Furthermore, the bla OXA23-like genes of Acinetobacter were detected using SYBR Green-based quantitative PCR in 13 (35%) of 37 water samples, including the 16 samples that were analyzed for next-generation sequencing, with concentrations ranging 5.3-7.5logcopies/100mL. There was no sufficient correlation found between fecal indicator bacteria, such as Escherichia coli and total coliforms, and potential pathogenic bacteria, as well as the bla OXA23-like gene of Acinetobacter. These results suggest the limitation of using conventional fecal indicator bacteria in evaluating the pathogenic bacteria contamination of different water sources in the Kathmandu Valley. Copyright © 2017 Elsevier B.V. All rights reserved.

  15. Reproducible Biofilm Cultivation of Chemostat-Grown Escherichia coli and Investigation of Bacterial Adhesion on Biomaterials Using a Non-Constant-Depth Film Fermenter

    Science.gov (United States)

    Lüdecke, Claudia; Jandt, Klaus D.; Siegismund, Daniel; Kujau, Marian J.; Zang, Emerson; Rettenmayr, Markus; Bossert, Jörg; Roth, Martin

    2014-01-01

    Biomaterials-associated infections are primarily initiated by the adhesion of microorganisms on the biomaterial surfaces and subsequent biofilm formation. Understanding the fundamental microbial adhesion mechanisms and biofilm development is crucial for developing strategies to prevent such infections. Suitable in vitro systems for biofilm cultivation and bacterial adhesion at controllable, constant and reproducible conditions are indispensable. This study aimed (i) to modify the previously described constant-depth film fermenter for the reproducible cultivation of biofilms at non-depth-restricted, constant and low shear conditions and (ii) to use this system to elucidate bacterial adhesion kinetics on different biomaterials, focusing on biomaterials surface nanoroughness and hydrophobicity. Chemostat-grown Escherichia coli were used for biofilm cultivation on titanium oxide and investigating bacterial adhesion over time on titanium oxide, poly(styrene), poly(tetrafluoroethylene) and glass. Using chemostat-grown microbial cells (single-species continuous culture) minimized variations between the biofilms cultivated during different experimental runs. Bacterial adhesion on biomaterials comprised an initial lag-phase I followed by a fast adhesion phase II and a phase of saturation III. With increasing biomaterials surface nanoroughness and increasing hydrophobicity, adhesion rates increased during phases I and II. The influence of materials surface hydrophobicity seemed to exceed that of nanoroughness during the lag-phase I, whereas it was vice versa during adhesion phase II. This study introduces the non-constant-depth film fermenter in combination with a chemostat culture to allow for a controlled approach to reproducibly cultivate biofilms and to investigate bacterial adhesion kinetics at constant and low shear conditions. The findings will support developing and adequate testing of biomaterials surface modifications eventually preventing biomaterial

  16. Non-invasive shallow seismic source comparison for hazardous waste site investigations

    International Nuclear Information System (INIS)

    Doll, W.E.

    1994-01-01

    Many commonly used shallow seismic sources are unacceptable for hazardous waste site investigations because they risk exhumation of contaminants in the soil, they add contaminants (e.g. lead) which are not allowed by regulations, or they add new migration paths for contaminants. Furthermore, recently developed high frequency vibrators for shallow investigations could be more effective at some sites than non-invasive impulsive sources because of their ability to tailor the source spectrum and reduce interference. The authors show preliminary results of a comparison test of eight non-invasive impulsive and swept sources in preparation for seismic reflection profiling on the Oak Ridge Reservation, Tennessee. Well log data are used to determine geologic contacts and to generate synthetic seismograms for the site. Common midpoint (CMP) seismic data for each source were collected at 95 geophone groups from 125 shot points along a 400m test line. Hydrophone data were obtained at 1.5m spacing between 61m and 133m depth in a hole near the center of the CMP line. As of March, 1994, brute stacks have been completed for three of the eight sources. Depth penetration is demonstrated in brute stacks and shot gathers, which show a 200ms reflector for all of the sources tested along portions of the line. Source effectiveness will also be evaluated by comparing images of several shallower reflectors (40--150ms) which are apparent in many of the records. Imaging of these reflectors appears to depend upon the ability of the source to generate sufficient high frequency energy (>100 Hz)

  17. Application of Multi-Source Remote Sensing Image in Yunnan Province Grassland Resources Investigation

    Science.gov (United States)

    Li, J.; Wen, G.; Li, D.

    2018-04-01

    Trough mastering background information of Yunnan province grassland resources utilization and ecological conditions to improves grassland elaborating management capacity, it carried out grassland resource investigation work by Yunnan province agriculture department in 2017. The traditional grassland resource investigation method is ground based investigation, which is time-consuming and inefficient, especially not suitable for large scale and hard-to-reach areas. While remote sensing is low cost, wide range and efficient, which can reflect grassland resources present situation objectively. It has become indispensable grassland monitoring technology and data sources and it has got more and more recognition and application in grassland resources monitoring research. This paper researches application of multi-source remote sensing image in Yunnan province grassland resources investigation. First of all, it extracts grassland resources thematic information and conducts field investigation through BJ-2 high space resolution image segmentation. Secondly, it classifies grassland types and evaluates grassland degradation degree through high resolution characteristics of Landsat 8 image. Thirdly, it obtained grass yield model and quality classification through high resolution and wide scanning width characteristics of MODIS images and sample investigate data. Finally, it performs grassland field qualitative analysis through UAV remote sensing image. According to project area implementation, it proves that multi-source remote sensing data can be applied to the grassland resources investigation in Yunnan province and it is indispensable method.

  18. Investigating sources and pathways of perfluoroalkyl acids (PFAAs) in aquifers in Tokyo using multiple tracers

    International Nuclear Information System (INIS)

    Kuroda, Keisuke; Murakami, Michio; Oguma, Kumiko; Takada, Hideshige; Takizawa, Satoshi

    2014-01-01

    We employed a multi-tracer approach to investigate sources and pathways of perfluoroalkyl acids (PFAAs) in urban groundwater, based on 53 groundwater samples taken from confined aquifers and unconfined aquifers in Tokyo. While the median concentrations of groundwater PFAAs were several ng/L, the maximum concentrations of perfluorooctane sulfonate (PFOS, 990 ng/L), perfluorooctanoate (PFOA, 1800 ng/L) and perfluorononanoate (PFNA, 620 ng/L) in groundwater were several times higher than those of wastewater and street runoff reported in the literature. PFAAs were more frequently detected than sewage tracers (carbamazepine and crotamiton), presumably owing to the higher persistence of PFAAs, the multiple sources of PFAAs beyond sewage (e.g., surface runoff, point sources) and the formation of PFAAs from their precursors. Use of multiple methods of source apportionment including principal component analysis–multiple linear regression (PCA–MLR) and perfluoroalkyl carboxylic acid ratio analysis highlighted sewage and point sources as the primary sources of PFAAs in the most severely polluted groundwater samples, with street runoff being a minor source (44.6% sewage, 45.7% point sources and 9.7% street runoff, by PCA–MLR). Tritium analysis indicated that, while young groundwater (recharged during or after the 1970s, when PFAAs were already in commercial use) in shallow aquifers (< 50 m depth) was naturally highly vulnerable to PFAA pollution, PFAAs were also found in old groundwater (recharged before the 1950s, when PFAAs were not in use) in deep aquifers (50–500 m depth). This study demonstrated the utility of multiple uses of tracers (pharmaceuticals and personal care products; PPCPs, tritium) and source apportionment methods in investigating sources and pathways of PFAAs in multiple aquifer systems. - Highlights: • Aquifers in Tokyo had high levels of perfluoroalkyl acids (up to 1800 ng/L). • PFAAs were more frequently detected than sewage

  19. Investigating sources and pathways of perfluoroalkyl acids (PFAAs) in aquifers in Tokyo using multiple tracers

    Energy Technology Data Exchange (ETDEWEB)

    Kuroda, Keisuke, E-mail: keisukekr@gmail.com [Department of Urban Engineering, Graduate School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo, Tokyo 113-8656 (Japan); Murakami, Michio [Institute of Industrial Science, The University of Tokyo, 4-6-1 Komaba, Meguro, Tokyo 153-8505 (Japan); Oguma, Kumiko [Department of Urban Engineering, Graduate School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo, Tokyo 113-8656 (Japan); Takada, Hideshige [Laboratory of Organic Geochemistry (LOG), Institute of Symbiotic Science and Technology, Tokyo University of Agriculture and Technology, Fuchu, Tokyo 183-8509 (Japan); Takizawa, Satoshi [Department of Urban Engineering, Graduate School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo, Tokyo 113-8656 (Japan)

    2014-08-01

    We employed a multi-tracer approach to investigate sources and pathways of perfluoroalkyl acids (PFAAs) in urban groundwater, based on 53 groundwater samples taken from confined aquifers and unconfined aquifers in Tokyo. While the median concentrations of groundwater PFAAs were several ng/L, the maximum concentrations of perfluorooctane sulfonate (PFOS, 990 ng/L), perfluorooctanoate (PFOA, 1800 ng/L) and perfluorononanoate (PFNA, 620 ng/L) in groundwater were several times higher than those of wastewater and street runoff reported in the literature. PFAAs were more frequently detected than sewage tracers (carbamazepine and crotamiton), presumably owing to the higher persistence of PFAAs, the multiple sources of PFAAs beyond sewage (e.g., surface runoff, point sources) and the formation of PFAAs from their precursors. Use of multiple methods of source apportionment including principal component analysis–multiple linear regression (PCA–MLR) and perfluoroalkyl carboxylic acid ratio analysis highlighted sewage and point sources as the primary sources of PFAAs in the most severely polluted groundwater samples, with street runoff being a minor source (44.6% sewage, 45.7% point sources and 9.7% street runoff, by PCA–MLR). Tritium analysis indicated that, while young groundwater (recharged during or after the 1970s, when PFAAs were already in commercial use) in shallow aquifers (< 50 m depth) was naturally highly vulnerable to PFAA pollution, PFAAs were also found in old groundwater (recharged before the 1950s, when PFAAs were not in use) in deep aquifers (50–500 m depth). This study demonstrated the utility of multiple uses of tracers (pharmaceuticals and personal care products; PPCPs, tritium) and source apportionment methods in investigating sources and pathways of PFAAs in multiple aquifer systems. - Highlights: • Aquifers in Tokyo had high levels of perfluoroalkyl acids (up to 1800 ng/L). • PFAAs were more frequently detected than sewage

  20. Biosurfactant production from marine hydrocarbon-degrading consortia and pure bacterial strains using crude oil as carbon source

    Directory of Open Access Journals (Sweden)

    Eleftheria eAntoniou

    2015-04-01

    Full Text Available Biosurfactants (BS are green amphiphilic molecules produced by microorganisms during biodegradation, increasing the bioavailability of organic pollutants. In this work, the BS production yield of marine hydrocarbon degraders isolated from Elefsina bay in Eastern Mediterranean Sea has been investigated. The drop collapse test was used as a preliminary screening test to confirm biosurfactant producing strains or mixed consortia. The community structure of the best consortia based on the drop collapse test was determined by 16S-rDNA pyrotag screening. Subsequently, the effect of incubation time, temperature, substrate and supplementation with inorganic nutrients, on biosurfactant production, was examined. Two types of BS - lipid mixtures were extracted from the culture broth; the low molecular weight BS Rhamnolipids and Sophorolipids. Crude extracts were purified by silica gel column chromatography and then identified by thin layer chromatography (TLC and Fourier transform infrared spectroscopy (FT-IR. Results indicate that biosurfactant production yield remains constant and low while it is independent of the total culture biomass, carbon source, and temperature. A constant BS concentration in a culture broth with continuous degradation of crude oil implies that the BS producing microbes generate no more than the required amount of biosurfactants that enables biodegradation of the crude oil. Isolated pure strains were found to have higher specific production yields than the complex microbial marine community-consortia. The heavy oil fraction of crude oil has emerged as a promising substrate for BS production (by marine BS producers with fewer impurities in the final product. Furthermore, a particular strain isolated from sediments, Paracoccus marcusii, may be an optimal choice for bioremediation purposes as its biomass remains trapped in the hydrocarbon phase, not suffering from potential dilution effects by sea currents.

  1. Biosurfactant production from marine hydrocarbon-degrading consortia and pure bacterial strains using crude oil as carbon source.

    Science.gov (United States)

    Antoniou, Eleftheria; Fodelianakis, Stilianos; Korkakaki, Emmanouela; Kalogerakis, Nicolas

    2015-01-01

    Biosurfactants (BSs) are "green" amphiphilic molecules produced by microorganisms during biodegradation, increasing the bioavailability of organic pollutants. In this work, the BS production yield of marine hydrocarbon degraders isolated from Elefsina bay in Eastern Mediterranean Sea has been investigated. The drop collapse test was used as a preliminary screening test to confirm BS producing strains or mixed consortia. The community structure of the best consortia based on the drop collapse test was determined by 16S-rDNA pyrotag screening. Subsequently, the effect of incubation time, temperature, substrate and supplementation with inorganic nutrients, on BS production, was examined. Two types of BS - lipid mixtures were extracted from the culture broth; the low molecular weight BS Rhamnolipids and Sophorolipids. Crude extracts were purified by silica gel column chromatography and then identified by thin layer chromatography and Fourier transform infrared spectroscopy. Results indicate that BS production yield remains constant and low while it is independent of the total culture biomass, carbon source, and temperature. A constant BS concentration in a culture broth with continuous degradation of crude oil (CO) implies that the BS producing microbes generate no more than the required amount of BSs that enables biodegradation of the CO. Isolated pure strains were found to have higher specific production yields than the complex microbial marine community-consortia. The heavy oil fraction of CO has emerged as a promising substrate for BS production (by marine BS producers) with fewer impurities in the final product. Furthermore, a particular strain isolated from sediments, Paracoccus marcusii, may be an optimal choice for bioremediation purposes as its biomass remains trapped in the hydrocarbon phase, not suffering from potential dilution effects by sea currents.

  2. Biosurfactant production from marine hydrocarbon-degrading consortia and pure bacterial strains using crude oil as carbon source

    Science.gov (United States)

    Antoniou, Eleftheria; Fodelianakis, Stilianos; Korkakaki, Emmanouela; Kalogerakis, Nicolas

    2015-01-01

    Biosurfactants (BSs) are “green” amphiphilic molecules produced by microorganisms during biodegradation, increasing the bioavailability of organic pollutants. In this work, the BS production yield of marine hydrocarbon degraders isolated from Elefsina bay in Eastern Mediterranean Sea has been investigated. The drop collapse test was used as a preliminary screening test to confirm BS producing strains or mixed consortia. The community structure of the best consortia based on the drop collapse test was determined by 16S-rDNA pyrotag screening. Subsequently, the effect of incubation time, temperature, substrate and supplementation with inorganic nutrients, on BS production, was examined. Two types of BS – lipid mixtures were extracted from the culture broth; the low molecular weight BS Rhamnolipids and Sophorolipids. Crude extracts were purified by silica gel column chromatography and then identified by thin layer chromatography and Fourier transform infrared spectroscopy. Results indicate that BS production yield remains constant and low while it is independent of the total culture biomass, carbon source, and temperature. A constant BS concentration in a culture broth with continuous degradation of crude oil (CO) implies that the BS producing microbes generate no more than the required amount of BSs that enables biodegradation of the CO. Isolated pure strains were found to have higher specific production yields than the complex microbial marine community-consortia. The heavy oil fraction of CO has emerged as a promising substrate for BS production (by marine BS producers) with fewer impurities in the final product. Furthermore, a particular strain isolated from sediments, Paracoccus marcusii, may be an optimal choice for bioremediation purposes as its biomass remains trapped in the hydrocarbon phase, not suffering from potential dilution effects by sea currents. PMID:25904907

  3. Investigation of gas discharge ion sources for on-line mass separation

    International Nuclear Information System (INIS)

    Kirchner, R.

    1976-03-01

    The development of efficient gas discharge ion sources with axial beam extraction for on-line mass separation is described. The aim of the investigation was to increase the ion source temperature, the lifetime and the ionisation yield in comparison to present low-pressure are discharge ion sources and to reduce the ion current density from usually 1 to 100 mA/cm 3 . In all ion sources the pressure range below the minimal ignition pressure of the arc discharge was investigated. As a result an ion source was developed which works at small changes in geometry and in electric device of a Nielsen source with high ionization yield (up to 50% for xenon) stabil and without ignition difficulties up to 10 -5 Torr. At a typical pressure of 3 x 10 -5 Torr ion current and ion current density are about 1 μA and 0.1 mA/cm 3 respectively besides high yield and a great emission aperture (diameter 1.2 mm). (orig.) [de

  4. Investigations into the Optimization of Multi-Source Strength Brachytherapy Treatment Procedures

    International Nuclear Information System (INIS)

    Henderson, D. L.; Yoo, S.; Thomadsen, B.R.

    2002-01-01

    The goal of this project is to investigate the use of multi-strength and multi-specie radioactive sources in permanent prostate implant brachytherapy. In order to fulfill the requirement for an optimal dose distribution, the prescribed dose should be delivered to the target in a nearly uniform dose distribution while simultaneously sparing sensitive structures. The treatment plan should use a small number of needles and sources while satisfying the treatment requirements. The hypothesis for the use of multi-strength and/or multi-specie sources is that a better treatment plan using fewer sources and needles could be obtained than by treatment plans using single-strength sources could reduce the overall number of sources used for treatment. We employ a recently developed greedy algorithm based on the adjoint concept as the optimization search engine. The algorithm utilizes and ''adjoint ratio'', which provides a means of ranking source positions, as the pseudo-objective function. It ha s been shown that the greedy algorithm can solve the optimization problem efficiently and arrives at a clinically acceptable solution in less than 10 seconds. Our study was inclusive, that is there was no combination of sources that clearly stood out from the others and could therefore be considered the preferred set of sources for treatment planning. Source strengths of 0.2 mCi (low), 0.4 mCi (medium), and 0.6 mCi (high) of 125 I in four different combinations were used for the multi-strength source study. The combination of high- and medium-strength sources achieved a more uniform target dose distribution due to few source implants whereas the combination of low-and medium-strength sources achieved better sparing of sensitive tissues including that of the single-strength 0.4 mCi base case. 125 I at 0.4 mCi and 192 Ir at 0.12 mCi and 0.25 mCi source strengths were used for the multi-specie source study. This study also proved inconclusive , Treatment plans using a combination of two 0

  5. Investigation of the process of vacuum freeze drying of bacterial concentrates for the meat industry with cryogenic freezing

    Directory of Open Access Journals (Sweden)

    V. V. Poymanov

    2016-01-01

    Full Text Available The research results of the nutritional value of the products manufactured are presented in the article. The main directions of bacterial concentrates application in the meat industry were determined. The analysis of starter cultures was given. The range of products manu-factured with bacterial concentrates was analyzed. It was shown that the introduction of innovative technologies will enable dynamic development of both large and small enterprises, which will create prerequisites for the growth of the Russian market of meat products. Economic efficiency of the studied substances treatment methods was proved. The relevance of the development of technology of pro-duction of dry bacterial concentrates with cryogenic freezing was proved. An integrated approach to the development of competitive domestic technologies and equipment for cryofreezing and sublimation dehydration by the use of granulation for the intensification of the internal heat and mass transfer, reducing specific energy consumption through the use of a combined cold supply system was pro-posed. Results of the study of the kinetics of the freezing process with the traditional method and cryofreezing are given in the paper. Rational parameters of the cryofreezing process were proposed. The optimum composition of cryoprotective medium was recommended. The research of the process of bacterial concentrate vacuum sublimation dehydration in the layer and granular form were conducted. The research confirmed that the use of the cryofreezing and granulation can increase the number of viable microorganisms in the bacterial concentrate and reduce the drying time. Rational vacuum sublimation dehydration modes were proposed. Methods of reduc-ing the defects of the processed products and improvement of the efficiency of production facilities were specified. Quality indicators of dried bacterial concentrates were given. The results obtained allow to carry out engineering calculations

  6. Sources of the Geomagnetic Field and the Modern Data That Enable Their Investigation

    DEFF Research Database (Denmark)

    Olsen, Nils; Hulot, Gauthier; Sabaka, Terence J.

    2014-01-01

    has a large and complicated magnetic field, a major part of which is produced by a self-sustaining dynamo operating in the fluid outer core. What is measured at or near the surface of the Earth, however, is the superposition of the core field and of additional fields caused by magnetized rocks......The geomagnetic field one can measure at the Earth’s surface or on board satellites is the sum of contributions from many different sources. These sources have different physical origins and can be found both below (in the form of electrical currents and magnetized material) and above (only...... in the form of electrical currents) the Earth’s surface. Each source happens to produce a contribution with rather specific spatio-temporal properties. This fortunate situation is what makes the identification and investigation of the contribution of each source possible, provided appropriate observational...

  7. A quasi-monochromatic X-rays source for art painting pigments investigation

    Energy Technology Data Exchange (ETDEWEB)

    Albertin, F.; Franconieri, A.; Gambaccini, M.; Petrucci, F.; Chiozzi, S. [University of Ferrara, Department of Physics and INFN, Ferrara (Italy); Moro, D. [University of Padova, Department of Physics, Padova (Italy); LNL - INFN, Legnaro, Padova (Italy)

    2009-08-15

    Monochromatic X-ray sources can be used for several applications, like in medicine or in studying our cultural heritage. We are investigating imaging systems based on a tuneable energy band X-ray source, to obtain an element mapping of painting layers using the K-edge technique. The narrow energy band beams are obtained with conventional X-ray source via Bragg diffraction on a mosaic crystal; such an analysis has been performed at different diffraction angles, tuning the energy to investigate spectra of interest from the artistic point of view, like zinc and copper. In this paper the characteristics of the system in terms of fluence rate are reported, and first results of this technique on canvas samples and painting are presented. (orig.)

  8. Investigations into the Optimization of Multi-Source Strength Brachytherapy Treatment Procedures

    CERN Document Server

    Henderson, D L; Yoo, S

    2002-01-01

    The goal of this project is to investigate the use of multi-strength and multi-specie radioactive sources in permanent prostate implant brachytherapy. In order to fulfill the requirement for an optimal dose distribution, the prescribed dose should be delivered to the target in a nearly uniform dose distribution while simultaneously sparing sensitive structures. The treatment plan should use a small number of needles and sources while satisfying the treatment requirements. The hypothesis for the use of multi-strength and/or multi-specie sources is that a better treatment plan using fewer sources and needles could be obtained than by treatment plans using single-strength sources could reduce the overall number of sources used for treatment. We employ a recently developed greedy algorithm based on the adjoint concept as the optimization search engine. The algorithm utilizes and ''adjoint ratio'', which provides a means of ranking source positions, as the pseudo-objective function. It ha s been shown that the gre...

  9. An Investigation of an Open-Source Software Development Environment in a Software Engineering Graduate Course

    Science.gov (United States)

    Ge, Xun; Huang, Kun; Dong, Yifei

    2010-01-01

    A semester-long ethnography study was carried out to investigate project-based learning in a graduate software engineering course through the implementation of an Open-Source Software Development (OSSD) learning environment, which featured authentic projects, learning community, cognitive apprenticeship, and technology affordances. The study…

  10. Investigation of Pseudo-Active State in Z-Source Inverter

    DEFF Research Database (Denmark)

    Schaltz, Erik; Oprea, Octavian; Larsen, Lasse

    2005-01-01

    This paper introduces a new operating state of the Z-source inverter denoted the pseudo-active state. The pseudoactive state is a state that may boost the output voltage of the Zsource inverter to a level higher than expected. The influence of the pseudo-active state is investigated and an equation...

  11. The Self-Taught Career Musician: Investigating Learning Sources and Experiences

    Science.gov (United States)

    Watson, Leah

    2016-01-01

    This article reports early findings from a qualitative study of 10 full-time musicians who are self-taught, to investigate their learning biographies. The aim is to identify, define and explore learning sources and experiences across the musician's learning biography. Conducted in Melbourne, Australia, the musicians were recruited through snowball…

  12. Energy intake and sources of energy intake in the European Prospective Investigation into Cancer and Nutrition

    NARCIS (Netherlands)

    Ocke, M. C.; Larranaga, N.; Grioni, S.; van den Berg, S. W.; Ferrari, P.; Salvini, S.; Benetou, V.; Linseisen, J.; Wirfalt, E.; Rinaldi, S.; Jenab, M.; Halkjaer, J.; Jakobsen, M. U.; Niravong, M.; Clavel-Chapelon, F.; Kaaks, R.; Bergmann, M.; Moutsiou, E.; Trichopoulou, A.; Lauria, C.; Sacerdote, C.; Bueno-de-Mesquita, H. B.; Peeters, P. H. M.; Hjartaker, A.; Parr, C. L.; Tormo, M. J.; Sanchez, M. J.; Manjer, J.; Hellstrom, V.; Mulligan, A.; Spencer, E. A.; Riboli, E.; Bingham, S.; Slimani, N.

    Objectives: To describe energy intake and its macronutrient and food sources among 27 regions in 10 countries participating in the European Prospective Investigation into Cancer and Nutrition (EPIC) study. Methods: Between 1995 and 2000, 36 034 subjects aged 35-74 years were administered a

  13. Investigation of ruminal bacterial diversity in dairy cattle fed supplementary monensin alone and in combination with fat, using pyrosequencing analysis.

    Science.gov (United States)

    Kim, M; Eastridge, M L; Yu, Z

    2014-02-01

    The objective of this study was to examine and compare the effects of monensin, both alone and together with dietary fat, on ruminal bacterial communities in dairy cattle fed the following 3 diets: a control diet, the control diet supplemented with monensin, and the control diet supplemented with both monensin and fat. Bacterial communities in the liquid and the adherent fractions of rumen content were analyzed using 454 pyrosequencing analysis of 16S rRNA gene amplicons. Most sequences were assigned to phyla Firmicutes and Bacteroidetes, irrespective of diets and fractions. Prevotella was the most dominant genus, but most sequences could not be classified at the genus level. The proportion of Gram-positive Firmicutes was reduced by 4.5% in response to monensin but increased by 12.8% by combination of monensin and fat, compared with the control diet. Some of the operational taxonomic units in Firmicutes and Bacteroidetes were also affected by monensin or by the combination of monensin with fat. The proportion of numerous bacteria potentially involved in lipolysis and (or) biohydrogenation was increased by both monensin and fat. The Shannon diversity index was decreased in the control diet supplemented with both monensin and fat, compared with the other 2 diet groups. Supplementary fats hinder bacterial attachment to plant particles and then result in decreased bacterial diversity in the rumen. The finding of this study may help in understanding the effect of monensin and fat on ruminant nutrition and the adverse effect of monensin and fat, such as milk fat depression and decreased feed digestibility.

  14. Investigation of specificity determinants in bacterial tRNA-guanine transglycosylase reveals queuine, the substrate of its eucaryotic counterpart, as inhibitor.

    Directory of Open Access Journals (Sweden)

    Inna Biela

    Full Text Available Bacterial tRNA-guanine transglycosylase (Tgt catalyses the exchange of the genetically encoded guanine at the wobble position of tRNAs(His,Tyr,Asp,Asn by the premodified base preQ1, which is further converted to queuine at the tRNA level. As eucaryotes are not able to synthesise queuine de novo but acquire it through their diet, eucaryotic Tgt directly inserts the hypermodified base into the wobble position of the tRNAs mentioned above. Bacterial Tgt is required for the efficient pathogenicity of Shigella sp, the causative agent of bacillary dysentery and, hence, it constitutes a putative target for the rational design of anti-Shigellosis compounds. Since mammalian Tgt is known to be indirectly essential to the conversion of phenylalanine to tyrosine, it is necessary to create substances which only inhibit bacterial but not eucaryotic Tgt. Therefore, it seems of utmost importance to study selectivity-determining features within both types of proteins. Homology models of Caenorhabditis elegans Tgt and human Tgt suggest that the replacement of Cys158 and Val233 in bacterial Tgt (Zymomonas mobilis Tgt numbering by valine and accordingly glycine in eucaryotic Tgt largely accounts for the different substrate specificities. In the present study we have created mutated variants of Z. mobilis Tgt in order to investigate the impact of a Cys158Val and a Val233Gly exchange on catalytic activity and substrate specificity. Using enzyme kinetics and X-ray crystallography, we gained evidence that the Cys158Val mutation reduces the affinity to preQ1 while leaving the affinity to guanine unaffected. The Val233Gly exchange leads to an enlarged substrate binding pocket, that is necessary to accommodate queuine in a conformation compatible with the intermediately covalently bound tRNA molecule. Contrary to our expectations, we found that a priori queuine is recognised by the binding pocket of bacterial Tgt without, however, being used as a substrate.

  15. Investigations of incorporating source directivity into room acoustics computer models to improve auralizations

    Science.gov (United States)

    Vigeant, Michelle C.

    Room acoustics computer modeling and auralizations are useful tools when designing or modifying acoustically sensitive spaces. In this dissertation, the input parameter of source directivity has been studied in great detail to determine first its effect in room acoustics computer models and secondly how to better incorporate the directional source characteristics into these models to improve auralizations. To increase the accuracy of room acoustics computer models, the source directivity of real sources, such as musical instruments, must be included in the models. The traditional method for incorporating source directivity into room acoustics computer models involves inputting the measured static directivity data taken every 10° in a sphere-shaped pattern around the source. This data can be entered into the room acoustics software to create a directivity balloon, which is used in the ray tracing algorithm to simulate the room impulse response. The first study in this dissertation shows that using directional sources over an omni-directional source in room acoustics computer models produces significant differences both in terms of calculated room acoustics parameters and auralizations. The room acoustics computer model was also validated in terms of accurately incorporating the input source directivity. A recently proposed technique for creating auralizations using a multi-channel source representation has been investigated with numerous subjective studies, applied to both solo instruments and an orchestra. The method of multi-channel auralizations involves obtaining multi-channel anechoic recordings of short melodies from various instruments and creating individual channel auralizations. These auralizations are then combined to create a total multi-channel auralization. Through many subjective studies, this process was shown to be effective in terms of improving the realism and source width of the auralizations in a number of cases, and also modeling different

  16. Effects of different nitrogen sources on the biogas production - a lab-scale investigation.

    Science.gov (United States)

    Wagner, Andreas Otto; Hohlbrugger, Peter; Lins, Philipp; Illmer, Paul

    2012-12-20

    For anaerobic digestion processes nitrogen sources are poorly investigated although they are known as possible process limiting factors (in the hydrolysis phase) but also as a source for fermentations for subsequent methane production by methanogenic archaea. In the present study different complex and defined nitrogen sources were investigated in a lab-scale experiment in order to study their potential to build up methane. The outcome of the study can be summarised as follows: from complex nitrogen sources yeast extract and casamino acids showed the highest methane production with approximately 600 ml methane per mole of nitrogen, whereas by the use of skim milk no methane production could be observed. From defined nitrogen sources L-arginine showed the highest methane production with almost 1400 ml methane per mole of nitrogen. Moreover it could be demonstrated that the carbon content and therefore C/N-ratio has only minor influence for the methane production from the used substrates. Copyright © 2011 Elsevier GmbH. All rights reserved.

  17. Advances in high-order harmonic generation sources for time-resolved investigations

    Energy Technology Data Exchange (ETDEWEB)

    Reduzzi, Maurizio [Dipartimento di Fisica, Politecnico di Milano, Piazza Leonardo da Vinci 32, 20133 Milano (Italy); Institute of Photonics and Nanotechnologies, CNR-IFN, Piazza Leonardo da Vinci 32, 20133 Milano (Italy); Carpeggiani, Paolo [Dipartimento di Fisica, Politecnico di Milano, Piazza Leonardo da Vinci 32, 20133 Milano (Italy); Kühn, Sergei [ELI-ALPS, ELI-Hu Kft., Dugonics ter 13, H-6720 Szeged (Hungary); Calegari, Francesca [Institute of Photonics and Nanotechnologies, CNR-IFN, Piazza Leonardo da Vinci 32, 20133 Milano (Italy); Nisoli, Mauro; Stagira, Salvatore [Dipartimento di Fisica, Politecnico di Milano, Piazza Leonardo da Vinci 32, 20133 Milano (Italy); Institute of Photonics and Nanotechnologies, CNR-IFN, Piazza Leonardo da Vinci 32, 20133 Milano (Italy); Vozzi, Caterina [Institute of Photonics and Nanotechnologies, CNR-IFN, Piazza Leonardo da Vinci 32, 20133 Milano (Italy); Dombi, Peter [ELI-ALPS, ELI-Hu Kft., Dugonics ter 13, H-6720 Szeged (Hungary); Wigner Research Center for Physics, 1121 Budapest (Hungary); Kahaly, Subhendu [ELI-ALPS, ELI-Hu Kft., Dugonics ter 13, H-6720 Szeged (Hungary); Tzallas, Paris; Charalambidis, Dimitris [ELI-ALPS, ELI-Hu Kft., Dugonics ter 13, H-6720 Szeged (Hungary); Foundation for Research and Technology – Hellas, Institute of Electronic Structure and Lasers, P.O. Box 1527, GR-711 10 Heraklion, Crete (Greece); Varju, Katalin [ELI-ALPS, ELI-Hu Kft., Dugonics ter 13, H-6720 Szeged (Hungary); Department of Optics and Quantum Electronics, University of Szeged, Dóm tér 9, 6720 Szeged (Hungary); Osvay, Karoly [ELI-ALPS, ELI-Hu Kft., Dugonics ter 13, H-6720 Szeged (Hungary); and others

    2015-10-15

    We review the main research directions ongoing in the development of extreme ultraviolet sources based on high-harmonic generation for the synthesization and application of trains and isolated attosecond pulses to time-resolved spectroscopy. A few experimental and theoretical works will be discussed in connection to well-established attosecond techniques. In this context, we present the unique possibilities offered for time-resolved investigations on the attosecond timescale by the new Extreme Light Infrastructure Attosecond Light Pulse Source, which is currently under construction.

  18. Advances in high-order harmonic generation sources for time-resolved investigations

    International Nuclear Information System (INIS)

    Reduzzi, Maurizio; Carpeggiani, Paolo; Kühn, Sergei; Calegari, Francesca; Nisoli, Mauro; Stagira, Salvatore; Vozzi, Caterina; Dombi, Peter; Kahaly, Subhendu; Tzallas, Paris; Charalambidis, Dimitris; Varju, Katalin; Osvay, Karoly

    2015-01-01

    We review the main research directions ongoing in the development of extreme ultraviolet sources based on high-harmonic generation for the synthesization and application of trains and isolated attosecond pulses to time-resolved spectroscopy. A few experimental and theoretical works will be discussed in connection to well-established attosecond techniques. In this context, we present the unique possibilities offered for time-resolved investigations on the attosecond timescale by the new Extreme Light Infrastructure Attosecond Light Pulse Source, which is currently under construction.

  19. Investigation of Grid-connected Voltage Source Converter Performance under Unbalanced Faults

    DEFF Research Database (Denmark)

    Jia, Jundi; Yang, Guangya; Nielsen, Arne Hejde

    2016-01-01

    Renewable energy sources (RES) and HVDC links are typically interfaced with the grid by power converters, whose performance during grid faults is significantly different from that of traditional synchronous generators. This paper investigates the performance of grid-connected voltage source...... that the performance of VSCs varies with their control strategies. Negative-sequence current control is necessary to restrict converter current in each phase under unbalanced faults. Among presented control strategies, the balanced current control strategy complies with the present voltage support requirement best...

  20. Upstream Freshwater and Terrestrial Sources Are Differentially Reflected in the Bacterial Community Structure along a Small Arctic River and Its Estuary

    Science.gov (United States)

    Hauptmann, Aviaja L.; Markussen, Thor N.; Stibal, Marek; Olsen, Nikoline S.; Elberling, Bo; Bælum, Jacob; Sicheritz-Pontén, Thomas; Jacobsen, Carsten S.

    2016-01-01

    Glacier melting and altered precipitation patterns influence Arctic freshwater and coastal ecosystems. Arctic rivers are central to Arctic water ecosystems by linking glacier meltwaters and precipitation with the ocean through transport of particulate matter and microorganisms. However, the impact of different water sources on the microbial communities in Arctic rivers and estuaries remains unknown. In this study we used 16S rRNA gene amplicon sequencing to assess a small river and its estuary on the Disko Island, West Greenland (69°N). Samples were taken in August when there is maximum precipitation and temperatures are high in the Disko Bay area. We describe the bacterial community through a river into the estuary, including communities originating in a glacier and a proglacial lake. Our results show that water from the glacier and lake transports distinct communities into the river in terms of diversity and community composition. Bacteria of terrestrial origin were among the dominating OTUs in the main river, while the glacier and lake supplied the river with water containing fewer terrestrial organisms. Also, more psychrophilic taxa were found in the community supplied by the lake. At the river mouth, the presence of dominant bacterial taxa from the lake and glacier was unnoticeable, but these taxa increased their abundances again further into the estuary. On average 23% of the estuary community consisted of indicator OTUs from different sites along the river. Environmental variables showed only weak correlations with community composition, suggesting that hydrology largely influences the observed patterns. PMID:27708629

  1. Upstream freshwater and terrestrial sources are differentially reflected in the bacterial community structure along a small Arctic river and its estuary

    Directory of Open Access Journals (Sweden)

    Aviaja Lyberth Hauptmann

    2016-09-01

    Full Text Available Glacier melting and altered precipitation patterns influence Arctic freshwater and coastal ecosystems. Arctic rivers are central to Arctic water ecosystems by linking glacier meltwaters and precipitation with the ocean through transport of particulate matter and microorganisms. However, the impact of different water sources on the microbial communities in Arctic rivers and estuaries remains unknown. In this study we used 16S rRNA gene amplicon sequencing to assess a small river and its estuary on the Disko Island, West Greenland (69°N. Samples were taken in August when there is maximum precipitation and temperatures are high in the Disko Bay area. We describe the bacterial community through a river into the estuary, including communities originating in a glacier and a proglacial lake. Our results show that water from the glacier and lake transports distinct communities into the river in terms of diversity and community composition. Bacteria of terrestrial origin were among the dominating OTUs in the main river, while the glacier and lake supplied the river with water containing fewer terrestrial organisms. Also, more psychrophilic taxa were found in the community supplied by the lake. At the river mouth, the presence of dominant bacterial taxa from the lake and glacier was unnoticeable, but these taxa increased their abundances again further into the estuary. On average 23% of the estuary community consisted of indicator OTUs from different sites along the river. Environmental variables showed only weak correlations with community composition, suggesting that hydrology largely influences the observed patterns.

  2. Sealed source and device design safety testing. Volume 4: Technical report on the findings of Task 4, Investigation of sealed source for paper mill digester

    International Nuclear Information System (INIS)

    Benac, D.J.; Iddings, F.A.

    1995-10-01

    This report covers the Task 4 activities for the Sealed Source and Device Safety testing program. SwRI was contracted to investigate a suspected leaking radioactive source that was installed in a gauge that was on a paper mill digester. The actual source that was leaking was not available, therefore, SwRI examined another source. SwRI concluded that the encapsulated source examined by SwRI was not leaking. However, the presence of Cs-137 on the interior and exterior of the outer encapsulation and hending tube suggests that contamination probably occurred when the source was first manufactured, then installed in the handling tube

  3. Partial Characterization of α-Galactosidic Activity from the Antarctic Bacterial Isolate, . LX-20 as a Potential Feed Enzyme Source

    Directory of Open Access Journals (Sweden)

    Inkyung Park

    2012-06-01

    Full Text Available An Antarctic bacterial isolate displaying extracellular α-galactosidic activity was named Paenibacillus sp. LX-20 based on 16S rRNA gene sequence analysis. Optimal activity for the LX-20 α-galactosidase occurred at pH 6.0–6.5 and 45°C. The enzyme immobilized on the smart polymer Eudragit L-100 retained 70% of its original activity after incubation for 30 min at 50°C, while the free enzyme retained 58% of activity. The enzyme had relatively high specificity for α-D-galactosides such as p-nitrophenyl-α-galactopyranoside, melibiose, raffinose and stachyose, and was resistant to some proteases such as trypsin, pancreatin and pronase. Enzyme activity was almost completely inhibited by Ag+, Hg2+, Cu2+, and sodium dodecyl sulfate, but activity was not affected by β-mercaptoethanol or EDTA. LX-20 α-galactosidase may be potentially useful as an additive for soybean processing in the feed industry.

  4. Bacterial synergism in lignocellulose biomass degradation : Complementary roles of degraders as influenced by complexity of the carbon source

    NARCIS (Netherlands)

    Cortes Tolalpa, Larisa; Falcao Salles, Joana; van Elsas, Jan

    2017-01-01

    Lignocellulosic biomass (LCB) is an attractive source of carbon for the production of sugars and other chemicals. Due to its inherent complexity and heterogeneity, efficient biodegradation requires the actions of different types of hydrolytic enzymes. In nature, complex microbial communities that

  5. Decay of Fecal Indicator Bacterial Populations and Bovine-Associated Source-Tracking Markers in Freshly Deposited Cow Pats

    Science.gov (United States)

    Understanding the survival of fecal indicator bacteria (FIB) and microbial source-tracking (MST) markers is critical to developing pathogen fate and transport models. Although pathogen survival in water microcosms and manure-amended soils is well documented, little is known about...

  6. Upstream Freshwater and Terrestrial Sources Are Differentially Reflected in the Bacterial Community Structure along a Small Arctic River and Its Estuary

    DEFF Research Database (Denmark)

    Hauptmann, Aviaja Zenia Edna Lyberth; Markussen, Thor N; Stibal, Marek

    2016-01-01

    of different water sources on the microbial communities in Arctic rivers and estuaries remains unknown. In this study we used 16S rRNA gene amplicon sequencing to assess a small river and its estuary on the Disko Island, West Greenland (69°N). Samples were taken in August when there is maximum precipitation......Glacier melting and altered precipitation patterns influence Arctic freshwater and coastal ecosystems. Arctic rivers are central to Arctic water ecosystems by linking glacier meltwaters and precipitation with the ocean through transport of particulate matter and microorganisms. However, the impact...... and temperatures are high in the Disko Bay area. We describe the bacterial community through a river into the estuary, including communities originating in a glacier and a proglacial lake. Our results show that water from the glacier and lake transports distinct communities into the river in terms of diversity...

  7. Marine organisms as source of extracts to disrupt bacterial communication: bioguided isolation and identification of quorum sensing inhibitors from Ircinia felix

    Directory of Open Access Journals (Sweden)

    Jairo Quintana

    Full Text Available AbstractIn this study, 39 extracts from marine organisms were evaluated as quorum sensing inhibitors, collected in the Colombian Caribbean Sea and the Brazilian Coast including 26 sponges, seven soft corals, five algae and one zooanthid. The results showed that crude extracts from the soft coral Eunicea laciniata, and the sponges Svenzea tubulosa, Ircinia felix and Neopetrosia carbonaria were the most promising source of quorum sensing inhibitors compounds without affecting bacterial growth, unlike the raw extracts of Agelas citrina, Agelas tubulata, Iotrochota arenosa, Topsentia ophiraphidites, Niphates caycedoi, Cliona tenuis, Ptilocaulis walpersi, Petrosia pellasarca, and the algae Laurencia catarinensis and Laurencia obtusa, which displayed potent antibacterial activity against the biosensors employed. The crude extract from the sponge I. felix was fractionated, obtaining furanosesterterpenes which were identified and evaluated as quorum sensing inhibitors, showing a moderate activity without affecting the biosensor's growth.

  8. Systematic investigation of background sources in neutron flux measurements with a proton-recoil silicon detector

    Energy Technology Data Exchange (ETDEWEB)

    Marini, P., E-mail: marini@cenbg.in2p3.fr [CENBG, CNRS/IN2P3-Université de Bordeaux, Chemin du Solarium B.P. 120, 33175 Gradignan (France); Mathieu, L. [CENBG, CNRS/IN2P3-Université de Bordeaux, Chemin du Solarium B.P. 120, 33175 Gradignan (France); Acosta, L. [Instituto de Física, Universidad Nacional Autónoma de México, Apartado Postal 20-364, México D.F. 01000 (Mexico); Aïche, M.; Czajkowski, S.; Jurado, B.; Tsekhanovich, I. [CENBG, CNRS/IN2P3-Université de Bordeaux, Chemin du Solarium B.P. 120, 33175 Gradignan (France)

    2017-01-01

    Proton-recoil detectors (PRDs), based on the well known standard H(n,p) elastic scattering cross section, are the preferred instruments to perform precise quasi-absolute neutron flux measurements above 1 MeV. The limitations of using a single silicon detector as PRD at a continuous neutron beam facility are investigated, with the aim of extending such measurements to neutron energies below 1 MeV. This requires a systematic investigation of the background sources affecting the neutron flux measurement. Experiments have been carried out at the AIFIRA facility to identify these sources. A study on the role of the silicon detector thickness on the background is presented and an energy limit on the use of a single silicon detector to achieve a neutron flux precision better than 1% is given.

  9. Selection of potent bacterial strain for over-production of PHB by using low cost carbon source for eco-friendly bioplastics

    Directory of Open Access Journals (Sweden)

    Rahat Abdul Rehman

    2015-11-01

    Full Text Available Background: The microbial PHB production is a promising tool for the plastic industry for the synthesis of environmental friendly, biodegradable plastic in contrast to the conventional petro-chemical based non-degradable plastics. The selection of potent bacterial strains, inexpensive carbon source, efficient fermentation and recovery processes are important aspects that were taken into account during this study. Methods: Different bacterial strains i.e. Bacillus Spp, P. putida and P. fluorescens were screened for maximum PHB production. Under media optimization, various carbon and nitrogen sources (alone or in combination were used to achieve the maximum PHB production. Finally the degradation tests of the PHB sheet were also performed to test its biodegradability potential. Results: Shake flask studies have shown the PHB concentrations upto 7.02, 4.50 and 34.4 mg/g of dry cell mass of P. putida, P. fluorescens and Bacillus Spp. respectively. Almost same results were observed at laboratory scale production of PHB in 10 L fermenter i.e. 6.28, 6.23 and 39.5 mg/g of dry cell mass by P. putida, P. fluorescens and Bacillus Spp. respectively. On the basis of these observations, Bacillus Spp. was chosen for laboratory scale PHB production. Corn steep liquor (4% was chosen as the best medium to achieve the highest PHB contents. Isolated PHB has shown biodegradation in soil up to 86.7% at 37oC. Conclusion: The Bacillus Spp. Proved to be the best strain for PHB production on only 4% CSL which is cheapest and easily available.

  10. Effect of bacterial root symbiosis and urea as source of nitrogen on performance of soybean plants grown hydroponically for Bioregenerative Life Support Systems (BLSSs).

    Science.gov (United States)

    Paradiso, Roberta; Buonomo, Roberta; Dixon, Mike A; Barbieri, Giancarlo; De Pascale, Stefania

    2015-01-01

    Soybean is traditionally grown in soil, where root symbiosis with Bradyrhizobium japonicum can supply nitrogen (N), by means of bacterial fixation of atmospheric N2. Nitrogen fertilizers inhibit N-fixing bacteria. However, urea is profitably used in soybean cultivation in soil, where urease enzymes of telluric microbes catalyze the hydrolysis to ammonium, which has a lighter inhibitory effect compared to nitrate. Previous researches demonstrated that soybean can be grown hydroponically with recirculating complete nitrate-based nutrient solutions. In Space, urea derived from crew urine could be used as N source, with positive effects in resource procurement and waste recycling. However, whether the plants are able to use urea as the sole source of N and its effect on root symbiosis with B. japonicum is still unclear in hydroponics. We compared the effect of two N sources, nitrate and urea, on plant growth and physiology, and seed yield and quality of soybean grown in closed-loop Nutrient Film Technique (NFT) in growth chamber, with or without inoculation with B. japonicum. Urea limited plant growth and seed yield compared to nitrate by determining nutrient deficiency, due to its low utilization efficiency in the early developmental stages, and reduced nutrients uptake (K, Ca, and Mg) throughout the whole growing cycle. Root inoculation with B. japonicum did not improve plant performance, regardless of the N source. Specifically, nodulation increased under fertigation with urea compared to nitrate, but this effect did not result in higher leaf N content and better biomass and seed production. Urea was not suitable as sole N source for soybean in closed-loop NFT. However, the ability to use urea increased from young to adult plants, suggesting the possibility to apply it during reproductive phase or in combination with nitrate in earlier developmental stages. Root symbiosis did not contribute significantly to N nutrition and did not enhance the plant ability to use

  11. Hormone-dependent bacterial growth, persistence and biofilm formation--a pilot study investigating human follicular fluid collected during IVF cycles.

    Directory of Open Access Journals (Sweden)

    Elise S Pelzer

    Full Text Available Human follicular fluid, considered sterile, is aspirated as part of an in vitro fertilization (IVF cycle. However, it is easily contaminated by the trans-vaginal collection route and little information exists in its potential to support the growth of microorganisms. The objectives of this study were to determine whether human follicular fluid can support bacterial growth over time, whether the steroid hormones estradiol and progesterone (present at high levels within follicular fluid contribute to the in vitro growth of bacterial species, and whether species isolated from follicular fluid form biofilms. We found that bacteria in follicular fluid could persist for at least 28 weeks in vitro and that the steroid hormones stimulated the growth of some bacterial species, specifically Lactobacillus spp., Bifidobacterium spp. Streptococcus spp. and E. coli. Several species, Lactobacillus spp., Propionibacterium spp., and Streptococcus spp., formed biofilms when incubated in native follicular fluids in vitro (18/24, 75%. We conclude that bacteria aspirated along with follicular fluid during IVF cycles demonstrate a persistent pattern of growth. This discovery is important since it can offer a new avenue for investigation in infertile couples.

  12. Using a Molecular-Genetic Approach to Investigate Bacterial Physiology in a Continuous, Research-Based, Semester-Long Laboratory for Undergraduates

    Directory of Open Access Journals (Sweden)

    Jeremiah Foster Ault

    2011-09-01

    Full Text Available Designing investigative laboratory exercises that encourage critical thinking, problem solving, and independent thought for upper-division biology courses is a difficult but worthwhile task. In an effort to do so, we developed a semester-long, continuous, research-based investigative laboratory that integrates numerous genetic and molecular biology methods into the investigation of a bacterial physiological process. In this lab, students use random Tn5 transposon mutagenesis to create prodigiosin pigment mutants in the bacterium, Serratia marcescens. This is followed by phenotypic characterization, cloning, and sequencing the Tn insertion site to identify genes involved in pigment biosynthesis. During this lab, students gain ample experience performing basic lab techniques while learning about — and applying — methods for elucidating gene function. The approach to the laboratory and the outcomes are intimately integrated into the teaching of many fundamental physiological processes underlying prodigiosin production in bacteria. The result is a cohesive course that integrates the theory and application of molecular genetic techniques with the study of bacterial physiology. Assessments of student learning objectives demonstrated that students greatly improved their understanding of both physiological processes and the genetic techniques used to investigate them. In addition, students felt that this semester-long exercise provided the necessary laboratory experience they needed and desired in preparation for careers in molecular biology, microbiology, and biochemistry.

  13. Investigating Ammonia Emission Sources in a Coastal Urban Air Shed Using Stable Isotope Techniques

    Science.gov (United States)

    Berner, A.; Felix, J. D. D.

    2017-12-01

    For nearly 100 years, mankind has met the food demands of a growing population by commercially producing and consuming reactive nitrogen fertilizers. So much so, that now 40-60% of the population relies on them. This increase has drastically altered the global nitrogen (N) cycle. Specifically, ammonia (NH3) emissions to the atmosphere have increased, resulting in wet and dry NHx (NH3 + NH4+) deposition products that can be substantial sources of N to sensitive ecosystems. Excess N can wreak havoc on these environments, causing soil acidification, water body eutrophication, and decreases in biodiversity. Despite these effects, NH3 remains generally unregulated in the U.S. Should policymakers elect to regulate NH3, quantification of NH3 emission sources and transport is essential. This has proven to be particularly difficult in urban regions, where ambient NH3 may result from local urban sources and/or NH3 transport from rural agricultural sources. The presented work investigates potential NH3 emission sources within a South Texas coastal urban air shed, Corpus Christi, TX, U.S.A. Previous work has shown an increasing fine particulate matter (PM2.5) trend within the region, which may be attributable to NH3 emissions from a variety of local sources, including vehicle traffic, shipping traffic, the petrochemical industry, and/or surrounding agricultural cropland and livestock. NH3 was collected monthly at a set of 8 sites within the Corpus Christi air shed, analyzed for NH3 concentration and N isotopic composition (d15N-NH3), and compared to known isotopic compositions of NH3 sources. Low and seasonally variable d15N-NH3 values are associated with varying agricultural sources (fertilizer, livestock waste, etc.), while higher and more seasonally constant d15N-NH3 values are associated with non-agricultural sources (vehicles, industry, etc.). Several other physical and chemical atmospheric components (e.g. SO2, NO2, O3, PM2.5, temperature, relative humidity) were also

  14. Use of DNA Markers for Investigating Sources of Bacteria in Contaminated Ground Water: Wooster Township, Wayne County, Ohio

    Science.gov (United States)

    Dumouchelle, Denise H.

    2006-01-01

    In 2004, a public-health nuisance was declared by the Wayne County Board of Health in the Scenic Heights Drive-Batdorf Road area of Wooster Township, Wayne County, Ohio, because of concerns about the safety of water from local wells. Repeated sampling had detected the presence of fecal-indicator bacteria and elevated nitrate concentrations. In June 2006, the U.S. Geological Survey (USGS), in cooperation with the Ohio Environmental Protection Agency (Ohio EPA), collected and analyzed samples from some of the affected wells to help investigate the possibility of human-origin bacterial contamination. Water samples from 12 wells and 5 home sewage-treatment systems (HSTS) were collected. Bromide concentrations were determined in samples from the 12 wells. Samples from 5 of the 12 wells were analyzed for wastewater compounds. Total coliform, enterococci and Escherichia coli (E. coli) bacteria concentrations were determined for samples from 8 of the 12 wells. In addition, two microbial source-tracking tools that employ DNA markers were used on samples from several wells and a composite sample of water from five septic tanks. The DNA markers from the Enterococcus faecium species and the order Bacteroidales are associated with specific sources, either human or ruminant sources. Bromide concentrations ranged from 0.04 to 0.18 milligrams per liter (mg/L). No wastewater compounds were detected at concentrations above the reporting limits. Samples from the 12 wells also were collected by Ohio EPA and analyzed for chloride and nitrate. Chloride concentrations ranged from 12.6 to 61.6 mg/L and nitrate concentrations ranged from 2.34 to 11.9 mg/L (as N). Total coliforms and enterococci were detected in samples from 8 wells, at concentrations from 2 to 200 colony-forming units per 100 milliliters (CFU/100 mL) and 0.5 to 17 CFU/100 mL, respectively. E. coli were detected in samples from three of the eight wells, at concentrations of 1 or 2 CFU/100 mL. Tests for the human

  15. Analytical investigation of low temperature lift energy conversion systems with renewable energy source

    International Nuclear Information System (INIS)

    Lee, Hoseong; Hwang, Yunho; Radermacher, Reinhard

    2014-01-01

    The efficiency of the renewable energy powered energy conversion system is typically low due to its moderate heat source temperature. Therefore, improving its energy efficiency is essential. In this study, the performance of the energy conversion system with renewable energy source was theoretically investigated in order to explore its design aspect. For this purpose, a computer model of n-stage low temperature lift energy conversion (LTLEC) system was developed. The results showed that under given operating conditions such as temperatures and mass flow rates of heat source and heat sink fluids the unit power generation of the system increased with the number of stage, and it became saturated when the number of staging reached four. Investigation of several possible working fluids for the optimum stage LTLEC system revealed that ethanol could be an alternative to ammonia. The heat exchanger effectiveness is a critical factor on the system performance. The power generation was increased by 7.83% for the evaporator and 9.94% for the condenser with 10% increase of heat exchanger effectiveness. When these low temperature source fluids are applied to the LTLEC system, the heat exchanger performance would be very critical and it has to be designed accordingly. - Highlights: •Energy conversion system with renewable energy is analytically investigated. •A model of multi-stage low temperature lift energy conversion systems was developed. •The system performance increases as the stage number is increased. •The unit power generation is increased with increase of HX effectiveness. •Ethanol is found to be a good alternative to ammonia

  16. Investigation of Biogenic and Non-biogenic Methane Sources in Texas

    Science.gov (United States)

    Yang, S.; Talbot, R. W.; Liu, L.; Lan, X.

    2016-12-01

    Methane (CH4) is a potent greenhouse gas with its mixing ratio increasing in the global atmosphere. Texas is one of the significant areas where underestimation of CH4 emissions by the U.S. inventories are pronounced. This study focused on two areas of Texas: Houston, the energy capital of the world, and the Barnett Shale area which is one of the largest onshore natural gas fields in the United States. The investigation of background CH4 and the fingerprints of CH4 emissions are critical to understanding potential impacts of extensive nature gas operations in these two areas. One-year of stationary CH4 measurements were studied to deduce the regional background CH4 characteristics and to identify the principle CH4 sources in Houston. Key information concerning CH4 sources can be obtained through the stable carbon isotope δ13CH4 during two field campaigns using a state-of-the-art mobile laboratory. CH4 mixing ratio and δ13C in CH4 were sampled by two cavity ring-down spectrometers (CRDS), one of which is coupled to a custom air core 13C sampling device which can supply more than 600 measurements for each plume analysis. Here, we report results of the overall δ13CH4 values and CH4 emission signatures derived from thirty-three sources in the two studied areas, ranging from oil production and processing, waste managements and landfills, to livestock. δ13CH4 signatures of these sources vary from -76‰ to -23‰. Several repeated measurements were sampled to investigate the variability of source signatures. We investigated a case of unexpected massive CH4 leaking detected near the San Jacinto River Fleet site. At the regional scale, the comparison of background δ13CH4 signatures were conducted to obtain the difference of dominate CH4 sources in two study areas. At the local scale, the combination of tower and mobile lab measurements were used to estimate and characterize CH4 emissions in Houston. The results and findings can supply valuable references for the local

  17. Well water quality in rural Nicaragua using a low-cost bacterial test and microbial source tracking.

    Science.gov (United States)

    Weiss, Patricia; Aw, Tiong Gim; Urquhart, Gerald R; Galeano, Miguel Ruiz; Rose, Joan B

    2016-04-01

    Water-related diseases, particularly diarrhea, are major contributors to morbidity and mortality in developing countries. Monitoring water quality on a global scale is crucial to making progress in terms of population health. Traditional analytical methods are difficult to use in many regions of the world in low-resource settings that face severe water quality issues due to the inaccessibility of laboratories. This study aimed to evaluate a new low-cost method (the compartment bag test (CBT)) in rural Nicaragua. The CBT was used to quantify the presence of Escherichia coli in drinking water wells and aimed to determine the source(s) of any microbial contamination. Results indicate that the CBT is a viable method for use in remote rural regions. The overall quality of well water in Pueblo Nuevo, Nicaragua was deemed unsafe, and results led to the conclusion that animal fecal wastes may be one of the leading causes of well contamination. Elevation and depth of wells were not found to impact overall water quality. However rope-pump wells had a 64.1% reduction in contamination when compared with simple wells.

  18. The Lag-Luminosity Relation in the GRB Source Frame: An Investigation with Swift BAT Bursts

    Science.gov (United States)

    Ukwatta, T. N.; Dhuga, K. S.; Stamatikos, M.; Dermer, C. D.; Sakamoto, T.; Sonbas, E.; Parke, W. C.; Maximon, L. C.; Linnemann, J. T.; Bhat, P. N.; hide

    2011-01-01

    Spectral lag. which is defined as the difference in time of arrival of high- and low-energy photons. is a common feature in gamma-ray bursts (GRBs). Previous investigations have shown a correlation between this lag and the isotropic peak luminosity for long duration bursts. However. most of the previous investigations used lags extracted in the observer frame only. In this work (based on a sample of 43 Swift long GRBs with known redshifts). we present an analysis of the lag-luminosity relation in the GRB source frame. Our analysis indicates a higher degree of correlation -0.82 +/- 0.05 (chance probability of approx. 5.5 x 10(exp -5) between the spectral lag and the isotropic peak luminosity, L(sub iso). with a best-fitting power-law index of -1.2 +/- 0.2. In addition, there is an anticorrelation between the source-frame spectral lag and the source-frame peak energy of the burst spectrum.

  19. Methylobacterium genome sequences: a reference blueprint to investigate microbial metabolism of C1 compounds from natural and industrial sources.

    Science.gov (United States)

    Vuilleumier, Stéphane; Chistoserdova, Ludmila; Lee, Ming-Chun; Bringel, Françoise; Lajus, Aurélie; Zhou, Yang; Gourion, Benjamin; Barbe, Valérie; Chang, Jean; Cruveiller, Stéphane; Dossat, Carole; Gillett, Will; Gruffaz, Christelle; Haugen, Eric; Hourcade, Edith; Levy, Ruth; Mangenot, Sophie; Muller, Emilie; Nadalig, Thierry; Pagni, Marco; Penny, Christian; Peyraud, Rémi; Robinson, David G; Roche, David; Rouy, Zoé; Saenampechek, Channakhone; Salvignol, Grégory; Vallenet, David; Wu, Zaining; Marx, Christopher J; Vorholt, Julia A; Olson, Maynard V; Kaul, Rajinder; Weissenbach, Jean; Médigue, Claudine; Lidstrom, Mary E

    2009-01-01

    Methylotrophy describes the ability of organisms to grow on reduced organic compounds without carbon-carbon bonds. The genomes of two pink-pigmented facultative methylotrophic bacteria of the Alpha-proteobacterial genus Methylobacterium, the reference species Methylobacterium extorquens strain AM1 and the dichloromethane-degrading strain DM4, were compared. The 6.88 Mb genome of strain AM1 comprises a 5.51 Mb chromosome, a 1.26 Mb megaplasmid and three plasmids, while the 6.12 Mb genome of strain DM4 features a 5.94 Mb chromosome and two plasmids. The chromosomes are highly syntenic and share a large majority of genes, while plasmids are mostly strain-specific, with the exception of a 130 kb region of the strain AM1 megaplasmid which is syntenic to a chromosomal region of strain DM4. Both genomes contain large sets of insertion elements, many of them strain-specific, suggesting an important potential for genomic plasticity. Most of the genomic determinants associated with methylotrophy are nearly identical, with two exceptions that illustrate the metabolic and genomic versatility of Methylobacterium. A 126 kb dichloromethane utilization (dcm) gene cluster is essential for the ability of strain DM4 to use DCM as the sole carbon and energy source for growth and is unique to strain DM4. The methylamine utilization (mau) gene cluster is only found in strain AM1, indicating that strain DM4 employs an alternative system for growth with methylamine. The dcm and mau clusters represent two of the chromosomal genomic islands (AM1: 28; DM4: 17) that were defined. The mau cluster is flanked by mobile elements, but the dcm cluster disrupts a gene annotated as chelatase and for which we propose the name "island integration determinant" (iid). These two genome sequences provide a platform for intra- and interspecies genomic comparisons in the genus Methylobacterium, and for investigations of the adaptive mechanisms which allow bacterial lineages to acquire methylotrophic

  20. An investigation of energy harvesting from renewable sources with PVDF and PZT

    International Nuclear Information System (INIS)

    Vatansever, D; Hadimani, R L; Shah, T; Siores, E

    2011-01-01

    Piezoelectric materials have been in use for many years; however, with an increasing concern about global warming, piezoelectricity has gained significant importance in research and development for extracting energy from the environment. In this work the voltage responses of ceramic based piezoelectric fibre composite structures (PFCs) and polymer based piezoelectric strips, PVDF (polyvinylidene fluoride), were evaluated when subjected to various wind speeds and water droplets in order to investigate the possibility of energy generation from these two natural renewable energy sources for utilization in low power electronic devices. The effects of material dimensions, drop mass, releasing height of the drops and wind speed on the voltage output were studied and the power was calculated. This work showed that piezoelectric polymer materials can generate higher voltage/power than ceramic based piezoelectric materials and it was proved that producing energy from renewable sources such as rain drops and wind is possible by using piezoelectric polymer materials

  1. Biosurfactant production from marine hydrocarbon-degrading consortia and pure bacterial strains using crude oil as carbon source

    OpenAIRE

    Antoniou, Eleftheria; Fodelianakis, Stilianos; Korkakaki, Emmanouela; Kalogerakis, Nicolas

    2015-01-01

    Biosurfactants (BS) are green amphiphilic molecules produced by microorganisms during biodegradation, increasing the bioavailability of organic pollutants. In this work, the BS production yield of marine hydrocarbon degraders isolated from Elefsina bay in Eastern Mediterranean Sea has been investigated. The drop collapse test was used as a preliminary screening test to confirm biosurfactant producing strains or mixed consortia. The community structure of the best consortia based on the drop c...

  2. Investigating the association of cardiovascular effects with personal exposure to particle components and sources

    Energy Technology Data Exchange (ETDEWEB)

    Wu, Chang-fu, E-mail: changfu@ntu.edu.tw [Department of Public Health, National Taiwan University, Taipei 100, Taiwan (China); Institute of Environmental Health, National Taiwan University, Taipei 100, Taiwan (China); Institute of Occupational Medicine and Industrial Hygiene, Taipei 100, Taiwan (China); Li, Ya-Ru; Kuo, I-Chun [Institute of Environmental Health, National Taiwan University, Taipei 100, Taiwan (China); Hsu, Shih-Chieh [Research Center for Environmental Changes, Academia Sinica, Taipei 115, Taiwan (China); Lin, Lian-Yu; Su, Ta-Chen [Department of Internal Medicine, National Taiwan University Hospital, Taipei 100, Taiwan (China)

    2012-08-01

    Background: Few studies included information on components and sources when exploring the cardiovascular health effects from personal exposure to particulate matters (PM). We previously reported that exposure to PM between 1.0 and 2.5 {mu}m (PM{sub 2.5-1}) was associated with increased cardio-ankle vascular index (CAVI, an arterial stiffness index), while exposure to PM smaller than 0.25 {mu}m (PM{sub 0.25}) decreased the heart rate variability (HRV) indices. The purpose of this study was to investigate the association between PM elements and cardiovascular health effects and identify responsible sources. Methods: In a panel study of seventeen mail carriers, the subjects were followed for 5-6 days while delivering mail outdoors. Personal filter samples of PM{sub 2.5-1} and PM{sub 0.25} were analyzed for their elemental concentrations. The source-specific exposures were further estimated by using absolute principal factor analysis. We analyzed the component- and source-specific health effects on HRV indices and CAVI using mixed models. Results: Several elements in PM{sub 2.5-1} (e.g., cadmium and strontium) were associated with the CAVI. Subsequent analyses showed that an interquartile range increase in exposure to PM from regional sources was significantly associated with a 3.28% increase in CAVI (95% confidence interval (CI), 1.47%-5.13%). This significant effect remained (3.35%, CI: 1.62%-5.11%) after controlling for the ozone exposures. For exposures to PM{sub 0.25}, manganese, calcium, nickel, and chromium were associated with the CAVI and/or the HRV indices. Conclusions: Our study suggests that PM{sub 2.5-1} and PM{sub 0.25} components may be associated with different cardiovascular effects. Health risks from exposure to PM from sources other than vehicle exhaust should not be underappreciated. - Highlights: Black-Right-Pointing-Pointer Increased arterial stiffness was related to the components in particles between 1.0 and 2.5 {mu}m. Black

  3. Bacterial meningitis

    NARCIS (Netherlands)

    Roos, Karen L.; van de Beek, Diederik

    2010-01-01

    Bacterial meningitis is a neurological emergency. Empiric antimicrobial and adjunctive therapy should be initiated as soon as a single set of blood cultures has been obtained. Clinical signs suggestive of bacterial meningitis include fever, headache, meningismus, vomiting, photophobia, and an

  4. Democracy, open source and music education? : A Deweyan investigation of music education in digital domains.

    OpenAIRE

    Thorgersen, Ketil

    2010-01-01

    Democracy, open source and music education? A Deweyan investigation of music education in digital domains.   Music has not been solely temporal for more than a century, and musical performance has not been created exclusively in real time by humans since the piano roll entered the stage in the late 19th century.  The mechanical, and later the digital, music industry has changed music as a social phenomena, increasing the availability of music to listen to, tools to create music with as well a...

  5. Investigating The Integral Control Rod Worth Of The Miniature Neutron Source Reactor MNSR

    International Nuclear Information System (INIS)

    Nguyen Hoang Hai; Do Quang Binh

    2011-01-01

    Determining control rod characteristics is an essential problem of nuclear reactor analysis. In this research, the integral control rod worth of the miniature neutron source reactor MNSR is investigated. Some other parameters of the nuclear reactor, such as core excess reactivity, shut down margin, are also calculated. Group constants for all reactor components are generated by the WIMSD code and then are used in the CITATION code to solve the neutron diffusion equations. The maximum relative error of the calculated results compared with the measurement data is about 3.5%. (author)

  6. Investigating The Neutron Flux Distribution Of The Miniature Neutron Source Reactor MNSR Type

    International Nuclear Information System (INIS)

    Nguyen Hoang Hai; Do Quang Binh

    2011-01-01

    Neutron flux distribution is the important characteristic of nuclear reactor. In this article, four energy group neutron flux distributions of the miniature neutron source reactor MNSR type versus radial and axial directions are investigated in case the control rod is fully withdrawn. In addition, the effect of control rod positions on the thermal neutron flux distribution is also studied. The group constants for all reactor components are generated by the WIMSD code, and the neutron flux distributions are calculated by the CITATION code. The results show that the control rod positions only affect in the planning area for distribution in the region around the control rod. (author)

  7. In vitro and in vivo investigation of bacterial cellulose dressing containing uniform silver sulfadiazine nanoparticles for burn wound healing

    Directory of Open Access Journals (Sweden)

    Xiaoxiao Wen

    2015-06-01

    Full Text Available Silver sulfadiazine (SSD particles in homogeneous dispersion state were prepared by an ultrasonic method and then nano- and microparticles were separated using centrifugation. SSD particles with narrow size distribution were impregnated with bacterial cellulose (BC to produce BC–SSD composite membrane used as burn wound dressing. A scanning electron microscope (SEM was used to examine the surface morphology of BC–SSD membranes. The incorporation of SSD in BC–SSD was confirmed by X-ray diffraction (XRD. Antimicrobial tests in vitro indicated that BC–SSD showed excellent antibacterial activity against Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli. The effects of BC–SSD on burn wound healing were assessed by rat models. The comparative study confirmed that the wound treated with BC–SSD showed high healing rate. The bacteria count in BC–SSD group was far less than control group. Histological analysis showed that epithelialization progressed better in wound treated with BC–SSD. These values demonstrated that the BC–SSD composite membrane could be a promising wound dressing for burn.

  8. Silver nanoparticle/bacterial cellulose gel membranes for antibacterial wound dressing: investigation in vitro and in vivo

    International Nuclear Information System (INIS)

    Wu, Jian; Zheng, Yudong; Wen, Xiaoxiao; Lin, Qinghua; Chen, Xiaohua; Wu, Zhigu

    2014-01-01

    Bacterial cellulose (BC) has attracted increasing attention as a novel wound dressing material, but its antimicrobial activity, which is one of the critical skin-barrier functions in wound healing, is not sufficient for use in practical applications. To overcome such a deficiency, silver nanoparticles were generated and self-assembled on the surface of BC nanofibers, forming a stable and evenly distributed Ag nanoparticle coated BC nanofiber (AgNP-BC). The performance of AgNP-BC was systematically studied in terms of antibacterial activities, cytocompatibility and effects on wound healing. The results showed that AgNP-BC exhibited significant antibacterial activity against Staphylococcus aureus. Moreover, AgNP-BC allowed attachment, and growth of rat fibroblasts with low cytotoxicity emerged. Based on these advantages, AgNP-BC samples were applied in a second-degree rat wound model. Wound flora showed a significant reduction during the healing. The fresh epidermal and dermis thicknesses with AgNP-BC samples were 111 and 855 µm respectively, higher than 74 and 619 µm for BC groups and 57 and 473 µm for untreated control wounds. The results demonstrated that AgNP-BC could reduce inflammation and promote scald wound healing. (paper)

  9. Source-case investigation of Mycobacterium wolinskyi cardiac surgical site infection.

    Science.gov (United States)

    Dupont, C; Terru, D; Aguilhon, S; Frapier, J-M; Paquis, M-P; Morquin, D; Lamy, B; Godreuil, S; Parer, S; Lotthé, A; Jumas-Bilak, E; Romano-Bertrand, S

    2016-07-01

    The non-tuberculous mycobacteria (NTM) Mycobacterium wolinskyi caused bacteraemia and massive colonization of an aortic prosthesis in a patient 16 days after cardiac surgery, necessitating repeat surgery and targeted antimicrobial chemotherapy. The infection control team investigated the source and conditions of infection. Peri-operative management of the patient complied with recommendations. The environmental investigation showed that although M. wolinskyi was not recovered, diverse NTM species were present in water from point-of-use taps and heater-cooler units for extracorporeal circulation. This case and increasing evidence of emerging NTM infections in cardiac surgery led to the implementation of infection control procedures in cardiac surgery wards. Copyright © 2016 The Healthcare Infection Society. Published by Elsevier Ltd. All rights reserved.

  10. Experimental investigation on water quality standard of Yangtze River water source heat pump.

    Science.gov (United States)

    Qin, Zenghu; Tong, Mingwei; Kun, Lin

    2012-01-01

    Due to the surface water in the upper reaches of Yangtze River in China containing large amounts of silt and algae, high content of microorganisms and suspended solids, the water in Yangtze River cannot be used for cooling a heat pump directly. In this paper, the possibility of using Yangtze River, which goes through Chongqing, a city in southwest China, as a heat source-sink was investigated. Water temperature and quality of the Yangtze River in the Chongqing area were analyzed and the performance of water source heat pump units in different sediment concentrations, turbidity and algae material conditions were tested experimentally, and the water quality standards, in particular surface water conditions, in the Yangtze River region that adapt to energy-efficient heat pumps were also proposed. The experimental results show that the coefficient of performance heat pump falls by 3.73% to the greatest extent, and the fouling resistance of cooling water in the heat exchanger increases up to 25.6% in different water conditions. When the sediment concentration and the turbidity in the river water are no more than 100 g/m3 and 50 NTU respectively, the performance of the heat pump is better, which can be used as a suitable river water quality standard for river water source heat pumps.

  11. Multipoint investigation of the source region of storm-time chorus

    Directory of Open Access Journals (Sweden)

    O. Santolík

    2004-07-01

    Full Text Available In this case study we investigate the source region of whistler-mode chorus located close to the geomagnetic equator at a radial distance of 4.4 Earth radii. We use measurements from the four Cluster spacecraft at separations of less than a few hundreds of km, recorded during the geomagnetic storm of 18 April 2002. The waveforms of the electric field fluctuations were obtained by the WBD instruments in the frequency range 50Hz-9.5kHz. Using these data, we calculate linear and rank correlation coefficients of the frequency averaged power-spectral density measured by the different spacecraft. Those coefficients have been recently shown to decrease with spacecraft separation distance perpendicular to the static magnetic field cchor03 with a characteristic scale length of 100km. We find this characteristic scale varying between 60 and 200km for different data intervals inside the source region. We examine possible explanations for the observed large scatter of the correlation coefficients, and we suggest a simultaneously acting effect of random positions of locations at which the individual chorus wave packets are generated. The statistical properties of the observations are approximately reproduced by a simple 2-D model of the source region, assuming a perpendicular half-width of 35km (approximately one wavelength of the whistler-mode waves for the distribution of power radiated from individual active areas.

  12. INR TRIGA Research Reactors: A Neutron Source for Radioisotopes and Materials Investigation

    International Nuclear Information System (INIS)

    Barbos, D.; Ciocanescu, M.; Paunoiu, C.; Bucsa, A.F.

    2013-01-01

    At the INR there are 2 high intensity neutron sources. These sources are in fact the two nuclear TRIGA reactors: TRIGA SSR 14 MW and TRIGA ACPR. TRIGA stationary reactor is provided with several in-core irradiation channels. Other several out-of-core irradiation channels are located in the vertical channels in the beryllium reflector blocks. The maximum value of the thermal neutron flux (E 14 cm -2 s -1 and of fast neutron flux (E>1 MeV) is 6.89×10 13 cm -2 s -1 . For neutron activation analysis both reactors are used and k0-NAA method has been implemented. At INR Pitesti a prompt gamma ray neutron activation analysis devices has been designed, manufactured ant put into operation. For nuclear materials properties investigation neutron radiography methods was developed in INR. For these purposes two neutron radiography devices were manufacture, one of them underwater and other one dry. The neutron beams are used for investigation of materials properties and components produced or under development for applications in the energy sector (fission and fusion). At TRIGA 14 MW reactor a neutron difractormeter and a SANS devices are available for material residual stress and texture measurements. TRIGA 14 MW reactor is used for medical and industrial radioisotopes production ( 131 I, 125 I, 192 Ir, etc) and a method for 99 Mo- 99 Tc production from fission is under developing. At INR Pitesti several special programmes for new types of nuclear fuel behavior characterization are under development. (author)

  13. Investigation of source location determination from Magsat magnetic anomalies: The Euler method approach

    Science.gov (United States)

    Ravat, Dhananjay

    1996-01-01

    The applicability of the Euler method of source location determination was investigated on several model situations pertinent to satellite-data scale situations as well as Magsat data of Europe. Our investigations enabled us to understand the end-member cases for which the Euler method will work with the present satellite magnetic data and also the cases for which the assumptions implicit in the Euler method will not be met by the present satellite magnetic data. These results have been presented in one invited lecture at the Indo-US workshop on Geomagnetism in Studies of the Earth's Interior in August 1994 in Pune, India, and at one presentation at the 21st General Assembly of the IUGG in July 1995 in Boulder, CO. A new method, called Anomaly Attenuation Rate (AAR) Method (based on the Euler method), was developed during this study. This method is scale-independent and is appropriate to locate centroids of semi-compact three dimensional sources of gravity and magnetic anomalies. The method was presented during 1996 Spring AGU meeting and a manuscript describing this method is being prepared for its submission to a high-ranking journal. The grant has resulted in 3 papers and presentations at national and international meetings and one manuscript of a paper (to be submitted shortly to a reputable journal).

  14. Postviral Complications: Bacterial Pneumonia.

    Science.gov (United States)

    Prasso, Jason E; Deng, Jane C

    2017-03-01

    Secondary bacterial pneumonia after viral respiratory infection remains a significant source of morbidity and mortality. Susceptibility is mediated by a variety of viral and bacterial factors, and complex interactions with the host immune system. Prevention and treatment strategies are limited to influenza vaccination and antibiotics/antivirals respectively. Novel approaches to identifying the individuals with influenza who are at increased risk for secondary bacterial pneumonias are urgently needed. Given the threat of further pandemics and the heightened prevalence of these viruses, more research into the immunologic mechanisms of this disease is warranted with the hope of discovering new potential therapies. Published by Elsevier Inc.

  15. Sources

    International Nuclear Information System (INIS)

    Duffy, L.P.

    1991-01-01

    This paper discusses the sources of radiation in the narrow perspective of radioactivity and the even narrow perspective of those sources that concern environmental management and restoration activities at DOE facilities, as well as a few related sources. Sources of irritation, Sources of inflammatory jingoism, and Sources of information. First, the sources of irritation fall into three categories: No reliable scientific ombudsman to speak without bias and prejudice for the public good, Technical jargon with unclear definitions exists within the radioactive nomenclature, and Scientific community keeps a low-profile with regard to public information. The next area of personal concern are the sources of inflammation. This include such things as: Plutonium being described as the most dangerous substance known to man, The amount of plutonium required to make a bomb, Talk of transuranic waste containing plutonium and its health affects, TMI-2 and Chernobyl being described as Siamese twins, Inadequate information on low-level disposal sites and current regulatory requirements under 10 CFR 61, Enhanced engineered waste disposal not being presented to the public accurately. Numerous sources of disinformation regarding low level radiation high-level radiation, Elusive nature of the scientific community, The Federal and State Health Agencies resources to address comparative risk, and Regulatory agencies speaking out without the support of the scientific community

  16. Bleeding sap and old wood are the two main sources of contamination of merging organs of vine plants by Xylophilus ampelinus, the causal agent of bacterial necrosis.

    Science.gov (United States)

    Grall, S; Roulland, C; Guillaumès, J; Manceau, C

    2005-12-01

    The spatial distribution of vine plants contaminated by Xylophilus ampelinus, the agent responsible for bacterial necrosis, was studied over a 5-year period within two vineyards in the Cognac area. Both vineyards were planted with Vitis vinifera cv. Ugni blanc but were different in age and agronomic location. The emission of X. ampelinus in contaminated bleeding sap was observed during vine sprouting. Contaminated bleeding sap is an important source of inoculum for external contamination due to the high susceptibility of young merging shoots to the pathogen. X. ampelinus emission by bleeding sap was not affected by the age of the plants or the location of the vineyards. However, its emission was irregular with time, and it varied between two fruit canes from individual plants and between plants as well as between years. Moreover, the two vineyards appeared to be entirely contaminated. Consequently, the behavior of the pathogen is not predictable. The distribution of the pathogen inside vine plant organs was analyzed through the four growing seasons. The old wood was contaminated throughout the year and constituted a stock inoculum for endophytic contamination of crude sap during the winter and the spring. Despite the fact that most of the young green shoots were contaminated in May, X.ampelinus was not found in green shoots in June and September, refuting the hypothesis of an epiphytic life of the pathogen under natural conditions. Although all plants were entirely contaminated in both vineyards, symptoms were rare and were observed on different plants each year.

  17. Energy intake and sources of energy intake in the European Prospective Investigation into Cancer and Nutrition.

    Science.gov (United States)

    Ocké, M C; Larrañaga, N; Grioni, S; van den Berg, S W; Ferrari, P; Salvini, S; Benetou, V; Linseisen, J; Wirfält, E; Rinaldi, S; Jenab, M; Halkjaer, J; Jakobsen, M U; Niravong, M; Clavel-Chapelon, F; Kaaks, R; Bergmann, M; Moutsiou, E; Trichopoulou, A; Lauria, C; Sacerdote, C; Bueno-de-Mesquita, H B; Peeters, P H M; Hjartåker, A; Parr, C L; Tormo, M J; Sanchez, M J; Manjer, J; Hellstrom, V; Mulligan, A; Spencer, E A; Riboli, E; Bingham, S; Slimani, N

    2009-11-01

    To describe energy intake and its macronutrient and food sources among 27 regions in 10 countries participating in the European Prospective Investigation into Cancer and Nutrition (EPIC) study. Between 1995 and 2000, 36 034 subjects aged 35-74 years were administered a standardized 24-h dietary recall. Intakes of macronutrients (g/day) and energy (kcal/day) were estimated using standardized national nutrient databases. Mean intakes were weighted by season and day of the week and were adjusted for age, height and weight, after stratification by gender. Extreme low- and high-energy reporters were identified using Goldberg's cutoff points (ratio of energy intake and estimated basal metabolic rate 2.72), and their effects on macronutrient and energy intakes were studied. Low-energy reporting was more prevalent in women than in men. The exclusion of extreme-energy reporters substantially lowered the EPIC-wide range in mean energy intake from 2196-2877 to 2309-2866 kcal among men. For women, these ranges were 1659-2070 and 1873-2108 kcal. There was no north-south gradient in energy intake or in the prevalence of low-energy reporting. In most centres, cereals and cereal products were the largest contributors to energy intake. The food groups meat, dairy products and fats and oils were also important energy sources. In many centres, the highest mean energy intakes were observed on Saturdays. These data highlight and quantify the variations and similarities in energy intake and sources of energy intake among 10 European countries. The prevalence of low-energy reporting indicates that the study of energy intake is hampered by the problem of underreporting.

  18. An investigation of sources of Campylobacter in a poultry production and packing operation in Barbados.

    Science.gov (United States)

    Workman, Suzanne N; Mathison, George E; Lavoie, Marc C

    2008-01-15

    Chicken meat is frequently contaminated with Campylobacter jejuni and is thought to be the major source of organisms causing human Campylobacter enteritis. Genotypic similarities between Campylobacter isolates from chicken meat at retail outlets and patients with gastroenteritis in Barbados suggested that it is a vehicle for infection of humans on the island and prompted this investigation of transmission of Campylobacter in a local poultry operation. Campylobacter testing was conducted at the hatchery, on the broiler farm and in the processing plant for two consecutive production cycles. The genetic relatedness of Campylobacter isolates was determined by RAPD typing with primer OPA 11. Hatchery samples and week-old chicks were negative for Campylobacter. Flocks became colonized as early as three weeks after introduction to the farm. Ten distinct RAPD genotypes were identified among isolates. Some genotypes were similar and may be of clonal origin. There was no evidence of vertical transmission of Campylobacter. The results suggest that the broiler flock was infected from more than one source in the farm environment.

  19. Investigations on microbial leaching of zircon by means of spark source mass spectrometry

    International Nuclear Information System (INIS)

    Becker, S.; Dietze, H.J.; Bullmann, M.; Iske, U.

    1985-01-01

    Spark source mass spectrometry is a useful method for chemical element analysis of geological and biological samples. This sensitive technique (detection limit down to the ppb-range) is used to analyze leaching processes by means of several microorganisms. The problem of microbial leaching of chemical resistent materials was tested under laboratory condition with regard to possible analytical and technical applications. Leaching of metalls with chemolithotrophic and heterotrophic, organic acids producing microorganisms has been investigated with zircon from Baltic Shield containing 0.7% rare earth elements and 1.67% hafnium. When zircon is leached with strains of Thiobacillus ferroxidans the rare earth elements, Hf, Th, and U mostly (about 80%) can be recovered. (author)

  20. Investigation on main source of dioxin analogues in human breast milk (second report)

    Energy Technology Data Exchange (ETDEWEB)

    Miyata, H.; Nakao, T.; Aozasa, O.; Ohta, S. [Setsunan Univ., Hirakata (Japan); Iwamatsu, T. [Teijin Eco Science, Co. Ltd., Matsuyama (Japan); Fujimine, Y. [Otsuka Pharmaceutical Co. Ltd., Tokushima (Japan); Fukui, S. [Fukui Lactation Consultation, Amagasaki (Japan)

    2004-09-15

    In many countries, the breast milk sample has been used as a suitable indicator in order to examine human exposure level to Dioxins. In general, the breast milk level is considered to be reflecting to their accumulation level in the body. In addition, it is considered that ca. 60% of the accumulation amount of Dioxins is excreted to the baby through breast milk by nursing for a year. However, are these things true? In 1989, Frust et al. reported a time course of concentrations of Dioxins (abbreviated as Dioxins) in breast milk of one German during a period of 1 - 60 weeks after delivery. In the case of PCDFs, the level of 10 - 13 weeks after delivery was remarkably higher than that of 5 weeks. In addition, the PCBs level on the 10 to 13 weeks was also higher in comparison with on the 1 week. Thus, their pollution levels did not always decrease with a passing of time after childbirth. This suggests that all Dioxins in breast milk might be not derived from their storage in the body. Therefore, in 2001, we investigated the time alteration on the pollution level of Dioxins in breast milk from nine mothers and on their infants' daily intake of Dioxins by nursing. Consequently, it was revealed that the average daily intake of PCDD/DFs (PCDDs + PCDFs) was roughly constant during a period of 5 to 180 days after delivery. If all PCDD/DFs in breast milk are derived from only their body storage, the pollution level in milk must decrease in a linear course during a period of 5 to 180 days after delivery. However, thus linear decrease of pollution level was not observed in all tested mothers. These results indicated that PCDD/DFs in milk might be also delivered from other sources except for their storage in the body. Therefore, in this study, we tried to investigate the source of Dioxins in human breast milk.

  1. Effects of corn-based diet starch content and neutral detergent fiber source on lactation performance, digestibility, and bacterial protein flow in dairy cows.

    Science.gov (United States)

    Fredin, S M; Akins, M S; Ferraretto, L F; Shaver, R D

    2015-01-01

    An experiment was conducted to evaluate the effects of corn-based dietary starch content and source of neutral detergent fiber (NDF) on lactation performance, nutrient digestion, bacterial protein flow, and ruminal parameters in lactating dairy cows. Eight ruminally cannulated multiparous Holstein cows averaging 193±11d in milk were randomly assigned to treatments in a replicated 4×4 Latin square design with 21-d periods. Treatment diets were high corn grain (HCG; 38% corn silage, 19% dry ground corn, and 4% soy hulls), high soy hulls (HSH; 38% corn silage, 11% dry ground corn, and 13% soy hulls), high corn silage (HCS; 50% corn silage, 6% dry ground corn, and 4% soy hulls), and low corn silage (LCS; 29% corn silage, 15% corn, and 19% soy hulls). The HCG, HSH, HCS, and LCS diets contained 29, 23, 24, and 22% starch; 27, 32, 30, and 32% total NDF; and 21, 21, 25, and 17% forage NDF (dry matter basis), respectively. Mean dry matter intake and milk yield were unaffected by treatment. Cows fed LCS had reduced milk fat content compared with HSH and HCS. The concentration of milk urea nitrogen was greater for cows fed HCS compared with the other treatments. Total-tract digestion of NDF was reduced for cows fed the HCG diet. Total-tract starch digestion was increased for cows fed the HSH and HCS compared with HCG and LCS diets. Bacterial protein flow was unaffected by treatment. Ruminal ammonia concentration was reduced in cows fed the HCG and LCS diets compared with the HCS diet. Ruminal propionate increased and the acetate:propionate ratio decreased in cows fed the LCS diet compared with the HCS diet. Ruminal pH was greater for cows fed the HCS diet compared with cows fed the LCS diet. Diet digestibility and performance of mid- to late-lactation cows fed reduced-starch diets by partially replacing corn grain with soy hulls or corn silage was similar to or improved compared with cows fed a normal-starch diet. Copyright © 2015 American Dairy Science Association

  2. Effect of Sucrose as Carbon Source and Probiotic Administrations on Bacterial Population Dinamic and Water Quality in White Shrimp, Litopenaeus vannamei Culture

    Directory of Open Access Journals (Sweden)

    . Sukenda

    2007-07-01

    Full Text Available Disinfection and nutrient enrichment prior stocking of  post larvae in the pond will be affected on the growth and composition of microbe.  Attention should be taken to some factors related to deterministic and stochastic factors of aquaculture environment  in order to develop microbe community.  This study was performed to determine effect of sucrose and probiotic supplementation to shrimp culture pond on water quality profile and population dynamic on shrimp culture media.  The treatments were supplementation of sucrose as carbon source, probiotic, and sucrose + probiotic into 25 L culture medium containing white shrimp, Litopenaeus vannamei.  Shrimp were fed commercial diet containing 30% protein by 5% body weight every day.  The result of study showed that bacterial population was increased by increasing time of shrimp rearing.  Increased of bacterial population was contrary to DO value.   Bacteria grew was heterotrop and vibrio that its intensity varied during experiment.  Supplementation of sucrose supported proliferation of bacteria including heterotrop, probiotik and vibrio groups.  Specifically, interaction between probiotic bacteria and vibrio was also found.  The presence of probiotic bacteria showed a negative impact on vibrio population.  Further, development of bacteria in general was also implicated to fluctuation of ammonia concentration in pond. Keywords: carbon, sucrose, probiotic, white shrimp, Litopenaeus vannamei   ABSTRAK Kegiatan disinfeksi dan pengkayaan nutrien sebelum penebaran PL akan mempengaruhi pola pertumbuhan dan komposisi mikroba di tambak. Hal-hal yang perlu diperhatikan dalam pengembangan komunitas mikroba adalah faktor-faktor deterministic dan sthocastic masing-masing lingkungan budidaya. Penelitian ini dilakukan untuk mengetahui pengaruh pemberian bahan berkarbon (sukrosa dan probiotik di tambak terhadap profil kualitas air serta dinamika populasi pada perairan budidaya. Pada penelitian ini

  3. Investigation of fingerprints for small polar molecules by using a tunable monochromatic THz source

    Science.gov (United States)

    Sun, Hongqian

    Over the past 20 years, considerable efforts have been dedicated to the generation and the application of electromagnetic waves in the Terahertz (THz) regime. Among all of the proposed applications, the THz spectroscopy is probably the most mature and promising one. According to the previous reports, the THz spectroscopy has been extensively applied into many analysis fields, including the investigation of vibrational modes for the crystalline solids, the characterization of electron transport in the condense matters and the identification of explosive materials at a standoff distance. More interestingly, since most gas phase chemicals exhibit unique transition peaks in the THz spectra, one could in principle achieve highly accurate molecular fingerprinting and chemical sensing as well. However, all of the practical THz spectroscopy applications were still greatly hampered by the lack of suitable sources and detectors. In this thesis, a unique approach to measure the THz spectrum is developed based on a novel tunable narrowband source. Unlike the previous THz systems, high power THz pulses were generated by the difference frequency generation processes between two collinearly propagated near infrared laser beams. To tune the output THz signal frequency, one can simply adjust one of the incident beam frequencies. Therefore, based on a convenient wavelength tuning scheme, the transmission spectra can be measured for a series of polar gases with either similar or distinct molecular structures. According to the measured spectra, it is found that the obtained transition frequencies, absorption intensities and molecular constants are all in good agreement with the theoretical results tabulated in the molecular spectroscopic databases, such as the HITRAN database. By further analyzing the transition frequencies, it is also discovered that one can confidently identify each polar molecule and differentiate between various isotopic variants based on their characteristic

  4. Methylobacterium genome sequences: a reference blueprint to investigate microbial metabolism of C1 compounds from natural and industrial sources.

    Directory of Open Access Journals (Sweden)

    Stéphane Vuilleumier

    Full Text Available Methylotrophy describes the ability of organisms to grow on reduced organic compounds without carbon-carbon bonds. The genomes of two pink-pigmented facultative methylotrophic bacteria of the Alpha-proteobacterial genus Methylobacterium, the reference species Methylobacterium extorquens strain AM1 and the dichloromethane-degrading strain DM4, were compared.The 6.88 Mb genome of strain AM1 comprises a 5.51 Mb chromosome, a 1.26 Mb megaplasmid and three plasmids, while the 6.12 Mb genome of strain DM4 features a 5.94 Mb chromosome and two plasmids. The chromosomes are highly syntenic and share a large majority of genes, while plasmids are mostly strain-specific, with the exception of a 130 kb region of the strain AM1 megaplasmid which is syntenic to a chromosomal region of strain DM4. Both genomes contain large sets of insertion elements, many of them strain-specific, suggesting an important potential for genomic plasticity. Most of the genomic determinants associated with methylotrophy are nearly identical, with two exceptions that illustrate the metabolic and genomic versatility of Methylobacterium. A 126 kb dichloromethane utilization (dcm gene cluster is essential for the ability of strain DM4 to use DCM as the sole carbon and energy source for growth and is unique to strain DM4. The methylamine utilization (mau gene cluster is only found in strain AM1, indicating that strain DM4 employs an alternative system for growth with methylamine. The dcm and mau clusters represent two of the chromosomal genomic islands (AM1: 28; DM4: 17 that were defined. The mau cluster is flanked by mobile elements, but the dcm cluster disrupts a gene annotated as chelatase and for which we propose the name "island integration determinant" (iid.These two genome sequences provide a platform for intra- and interspecies genomic comparisons in the genus Methylobacterium, and for investigations of the adaptive mechanisms which allow bacterial lineages to acquire

  5. sources

    Directory of Open Access Journals (Sweden)

    Shu-Yin Chiang

    2002-01-01

    Full Text Available In this paper, we study the simplified models of the ATM (Asynchronous Transfer Mode multiplexer network with Bernoulli random traffic sources. Based on the model, the performance measures are analyzed by the different output service schemes.

  6. Reconnaissance of the hydrology, water quality, and sources of bacterial and nutrient contamination in the Ozark Plateaus aquifer system and Cave Springs Branch of Honey Creek, Delaware County, Oklahoma, March 1999-March 2000

    Science.gov (United States)

    Schlottmann, Jamie L.; Tanner, Ralph S.; Samadpour, Mansour

    2000-01-01

    A reconnaissance investigation of hydrology and water quality was conducted to evaluate possible sources of bacteria and nutrient contamination in the Cave Springs Branch basin and the underlying karstic Ozark Plateau aquifer system. Objectives were to: (1) determine the directions of ground-water flow in the basin and determine whether Cave Springs Branch interacts with ground water, (2) compare water quality in Cave Springs Branch with water quality in nearby wells to determine whether the stream is contaminating nearby wells, and (3) determine sources of fecal coliform bacteria and nitrate contamination in Cave Springs Branch and ground water. Potential sources of bacteria and nitrate in the area include cultivated agriculture, cow and horse on pasture, poultry production, households, and wildlife. Presence of fecal coliform and fecal streptococcal bacteria directly indicate fecal contamination and the potential for the presence of other pathogenic organisms in a water supply. Nitrate in drinking water poses health risks and may indicate the presence of additional contaminants. Fecal coliform bacteria colony counts were least in wells, intermediate in the poultry-processing plant wastewater outfall and Honey Creek above the confluence with Cave Springs Branch, and greatest in Cave Springs Branch. Bacteria strains and resistance to antibiotics by some bacteria indicate that livestock may have been sources of some bacteria in the water samples. Multiple antibiotic resistances were not present in the isolates from the water samples, indicating that the bacteria may not be from human or poultry sources. Ribotyping indicates that Escherichia coli bacteria in water samples from the basin were from bird, cow, horse, dog, deer, and human sources. The presence of multiple ribotypes from each type of animal source except bird indicates that most of the bacteria are from multiple populations of source animals. Identifiable sources of bacteria in Cave Springs Branch at the

  7. Waste Area Grouping 2 Remedial Investigation Phase 1 Seep Task data report: Contaminant source area assessment

    International Nuclear Information System (INIS)

    Hicks, D.S.

    1996-03-01

    This report presents the findings of the Waste Area Grouping (WAG) 2, Phase 1 Remedial Investigation (RI) Seep Task efforts during 1993 and 1994 at Oak Ridge National Laboratory (ORNL). The results presented here follow results form the first year of sampling, 1992, which are contained in the Phase 1 RI report for WAG 2 (DOE 1995a). The WAG 2 Seep Task efforts focused on contaminants in seeps, tributaries, and main streams within the White Oak Creek (WOC) watershed. This report is designed primarily as a reference for contaminants and a resource for guiding remedial decisions. Additional in-depth assessments of the Seep Task data may provide clearer understandings of contaminant transport from the different source areas in the WOC watershed. WAG 2 consists of WOC and its tributaries downstream of the ORNL main plant area, White Oak Lake, the White Oak Creek Embayment of the Clinch River, and the associated flood plains and subsurface environment. The WOC watershed encompasses ORNL and associated WAGs. WAG 2 acts as an integrator for contaminant releases from the contaminated sites at ORNL and as the conduit transporting contaminants to the Clinch River. The main objectives of the Seep Task were to identify and characterize seeps, tributaries and source areas that are responsible for the contaminant releases to the main streams in WAG 2 and to quantify their input to the total contaminant release from the watershed at White Oak Dam (WOD). Efforts focused on 90 Sr, 3 H, and 137 Cs because these contaminants pose the greatest potential human health risk from water ingestion at WOD. Bimonthly sampling was conducted throughout the WOC watershed beginning in March 1993 and ending in August 1994. Samples were also collected for metals, anions, alkalinity, organics, and other radionuclides

  8. Molecular investigation on the binding of Cd(II) by the binary mixtures of montmorillonite with two bacterial species

    Energy Technology Data Exchange (ETDEWEB)

    Du, Huihui; Qu, ChenChen; Liu, Jing; Chen, Wenli; Cai, Peng; Shi, Zhihua; Yu, Xiao-Ying; Huang, Qiaoyun

    2017-10-01

    Bacteria and phyllosilicate commonly coexist in the natural environment, producing various bacteria–clay complexes that are capable of immobilizing heavy metals, such as cadmium, via adsorption. However, the molecular binding mechanisms of heavy metals on these complex aggregates still remain poorly understood. This study investigated Cd adsorption on Gram-positive B. subtilis, Gram-negative P. putida and their binary mixtures with montmorillonite (Mont) using the Cd K-edge x-ray absorption spectroscopy (XAS) and isothermal titration calorimetry (ITC). We observed a lower adsorptive capacity for P. putida than B. subtilis, whereas P. putida–Mont and B. subtilis–Mont mixtures showed nearly identical Cd adsorption behaviors. EXAFS fits and ITC measurements demonstrated more phosphoryl binding of Cd in P. putida. The decreased coordination of C atoms around Cd and the reduced adsorption enthalpies and entropies for the binary mixtures compared to that for individual bacteria suggested that the bidentate Cd-carboxyl complexes in pure bacteria systems were probably transformed into monodentate complexes that acted as ionic bridging structure between bacteria and motmorillonite. This study clarified the binding mechanism of Cd at the bacteria–phyllosilicate interfaces from a molecular and thermodynamic view, which has an environmental significance for predicting the chemical behavior of trace elements in complex mineral–organic systems.

  9. Investigation Of The Origin Of Various Water Sources In The Vicinity Of Ngancar Dam, Wonogiri Using Natural Isotopes

    International Nuclear Information System (INIS)

    Sidauruk, Paston; Indrojoyo; Wibagoyo; Pratikno, Bungkus; Evarista Ristin, P.I.

    2000-01-01

    The investigation of the origin of various water sources in the vicinity of Ngancar Dam, Wonogiri, using natural isotopes technique has been conducted. The study includes collecting and analyzing water samples from various sources in the vicinity of the dam such as reservoir water, water discharges, springs, local water well, rain water, water from piezometer and observation wells. For this investigation, natural isotopes composition and hydro chemical ions of the samples have been analyzed and interpreted. From the data interpretation, it is concluded that most of the water in various sources originated from water reservoir

  10. Investigation on the electron flux to the wall in the VENUS ion source

    Energy Technology Data Exchange (ETDEWEB)

    Thuillier, T., E-mail: thuillier@lpsc.in2p3.fr; Angot, J. [LPSC, Université Grenoble-Alpes, CNRS/IN2P3, 53 rue des Martyrs, 38026 Grenoble Cedex (France); Benitez, J. Y.; Hodgkinson, A.; Lyneis, C. M.; Todd, D. S.; Xie, D. Z. [Nuclear Science Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720 (United States)

    2016-02-15

    The long-term operation of high charge state electron cyclotron resonance ion sources fed with high microwave power has caused damage to the plasma chamber wall in several laboratories. Porosity, or a small hole, can be progressively created in the chamber wall which can destroy the plasma chamber over a few year time scale. A burnout of the VENUS plasma chamber is investigated in which the hole formation in relation to the local hot electron power density is studied. First, the results of a simple model assuming that hot electrons are fully magnetized and strictly following magnetic field lines are presented. The model qualitatively reproduces the experimental traces left by the plasma on the wall. However, it is too crude to reproduce the localized electron power density for creating a hole in the chamber wall. Second, the results of a Monte Carlo simulation, following a population of scattering hot electrons, indicate a localized high power deposited to the chamber wall consistent with the hole formation process. Finally, a hypervapotron cooling scheme is proposed to mitigate the hole formation in electron cyclotron resonance plasma chamber wall.

  11. Investigation of phosphorus atomization using high-resolution continuum source electrothermal atomic absorption spectrometry

    International Nuclear Information System (INIS)

    Dessuy, Morgana B.; Vale, Maria Goreti R.; Lepri, Fabio G.; Welz, Bernhard; Heitmann, Uwe

    2007-01-01

    The atomization of phosphorus in electrothermal atomic absorption spectrometry has been investigated using a high-resolution continuum source atomic absorption spectrometer and atomization from a graphite platform as well as from a tantalum boat inserted in a graphite tube. A two-step atomization mechanism is proposed for phosphorus, where the first step is a thermal dissociation, resulting in a fast atomization signal early in the atomization stage, and the second step is a slow release of phosphorus atoms from the graphite tube surface following the adsorption of molecular phosphorus at active sites of the graphite surface. Depending on experimental conditions only one of the mechanisms or both might be active. In the absence of a modifier and with atomization from a graphite or tantalum platform the second mechanism appears to be dominant, whereas in the presence of sodium fluoride as a modifier both mechanisms are observed. Intercalation of phosphorus into the graphite platform in the condensed phase has also been observed; this phosphorus, however, appears to be permanently trapped in the structure of the graphite and does not contribute to the absorption signal

  12. Investigation of the composition of an ion beam produced using a multi arc ion source

    Energy Technology Data Exchange (ETDEWEB)

    Engelko, V [Efremov Institute of Electrophysical Apparatus, St. Petersburg (Russian Federation); Giese, H; Schalk, S [Forschungszentrum Karlsruhe (Germany). INR; Mishin, M; Tsibin, O Y [St. Petersburg State Technical Univ. (Russian Federation)

    1997-12-31

    Complementing the diode and beam transport optimization studies currently performed at FZK Karlsruhe on the proton-beam-facility PROFA, supplementary investigations were run at the St. Petersburg State University, focusing on ion beam divergence and composition measurements using the TOF techniques. To ensure direct transferability of the results to the PROFA facility, these measurements were made on a scaled-down replica of the PROFA diode, comprising an array of small polyethylene flash-over plasma sources and a grid extraction system. Only the results of the beam composition measurements are presented, and the following conclusions are made: (i) The ion beam contains H and C ions and heavier constituents that remain to be identified. (ii) The beam composition changes significantly with the total number of shots: While H{sup +} ions predominated in the starting phase of the experiments, heavier components outweighed them later on, presumably due to hydrogen depletion of the surface layer of the anode polyethylene units. (iii) Reconditioning of the polyethylene units may be possible by running the diode at higher currents (self-cleaning) or by cutting off a surface layer. (author). 7 figs., 3 refs.

  13. Experimental investigation of nondipole effects in photoemission at the advanced light source

    International Nuclear Information System (INIS)

    Guillemin, Renaud; Hemmers, Oliver; Lindle, Dennis W.; Manson, Steven T.

    2005-01-01

    Breakdowns in the dipole approximation in the soft-X-ray photon-energy range (hν<5keV) were first observed 30 years ago and have been studied theoretically for many years. However, only recently their significance at low energies has been appreciated when advances in gas-phase-photoemission experiments using synchrotron radiation began to highlight numerous examples of significant nondipole effects at photon energies as low as tens of eV. In a previous publication [Hemmers et al., 2004a. Radiat. Phys. Chem. 70, 123-147], we presented a description of the recent advances made in the investigation of nondipole effects in photoionization from an experimental perspective. In this article, we report the results obtained by the X-ray atomic and molecular spectroscopy (XAMS) group at the Advanced Light Source, over the last 10 years, on the limits of the dipole approximation, probed by angle-resolved photoelectron spectroscopy for atoms and molecules in gas phase

  14. Synthesis, aggregation and spectroscopic studies of novel water soluble metal free, zinc, copper and magnesium phthalocyanines and investigation of their anti-bacterial properties

    Science.gov (United States)

    Bayrak, Rıza; Akçay, Hakkı Türker; Beriş, Fatih Şaban; Şahin, Ertan; Bayrak, Hacer; Demirbaş, Ümit

    2014-12-01

    In this study, novel phthalonitrile derivative (3) was synthesized by the reaction between 4-nitrophthalonitrile (2) and a triazole derivative (1) containing pyridine moiety. Crystal structure of compound (3) was characterized by X-ray diffraction. New metal free and metallo-phthalocyanine complexes (Zn, Cu, and Mg) were synthesized using the phthalonitrile derivative (3). Cationic derivatives of these phthalocyanines (5, 7, 9, and 11) were prepared from the non-ionic phthalocyanines (4, 6, 8, and 10). All proposed structures were supported by instrumental methods. The aggregation behaviors of the phthalocyanines (4-11) were investigated in different solvents such as dimethylsulfoxide (DMSO), N,N-dimethylformamide (DMF), chloroform and water. Water soluble cationic Pcs (5, 7, 9, and 11) aggregated in water and sodium dodecyl sulfate was used to prevent the aggregation. The second derivatives of the UV-Vis spectra of aggregated Pcs were used for analyzing the Q and B bands of aggregated species. Thermal behaviors of the phthalocyanines were also studied. In addition, anti-bacterial properties of the phthalocyanines were investigated. We used four gram negative and two gram positive bacteria to determine antibacterial activity of these compounds. Compound 7 has the best activity against the all bacteria with 125 μg/mL of MIC value. Compounds 4, 6, and 10 have the similar effect on the bacteria with 250 μg/mL of MIC value.

  15. Exogenous contrast agents for thermoacoustic imaging: An investigation into the underlying sources of contrast

    International Nuclear Information System (INIS)

    Ogunlade, Olumide; Beard, Paul

    2015-01-01

    Purpose: Thermoacoustic imaging at microwave excitation frequencies is limited by the low differential contrast exhibited by high water content tissues. To overcome this, exogenous thermoacoustic contrast agents based on gadolinium compounds, iron oxide, and single wall carbon nanotubes have previously been suggested and investigated. However, these previous studies did not fully characterize the electric, magnetic, and thermodynamic properties of these agents thus precluding identification of the underlying sources of contrast. To address this, measurements of the complex permittivity, complex permeability, DC conductivity, and Grüneisen parameter have been made. These measurements allowed the origins of the contrast provided by each substance to be identified. Methods: The electric and magnetic properties of the contrast agents were characterized at 3 GHz using two rectangular waveguide cavities. The DC conductivity was measured separately using a conductivity meter. Thermoacoustic signals were then acquired and compared to those generated in water. Finally, 3D electromagnetic simulations were used to decouple the different contributions to the absorbed power density. Results: It was found that the gadolinium compounds provided appreciable electric contrast but not originating from the gadolinium itself. The contrast was either due to dissociation of the gadolinium salt which increased ionic conductivity or its nondissociated polar fraction which increased dielectric polarization loss or a combination of both. In addition, very high concentrations were required to achieve appreciable contrast, to the extent that the Grüneisen parameter increased significantly and became a source of contrast. Iron oxide particles were found to produce low but measurable dielectric contrast due to dielectric polarization loss, but this is attributed to the coating of the particles not the iron oxide. Single wall carbon nanotubes did not provide measurable contrast of any type

  16. Exogenous contrast agents for thermoacoustic imaging: An investigation into the underlying sources of contrast

    Energy Technology Data Exchange (ETDEWEB)

    Ogunlade, Olumide, E-mail: o.ogunlade@ucl.ac.uk; Beard, Paul [Department of Medical Physics and Biomedical Engineering, University College London, London WC1E 6BT (United Kingdom)

    2015-01-15

    Purpose: Thermoacoustic imaging at microwave excitation frequencies is limited by the low differential contrast exhibited by high water content tissues. To overcome this, exogenous thermoacoustic contrast agents based on gadolinium compounds, iron oxide, and single wall carbon nanotubes have previously been suggested and investigated. However, these previous studies did not fully characterize the electric, magnetic, and thermodynamic properties of these agents thus precluding identification of the underlying sources of contrast. To address this, measurements of the complex permittivity, complex permeability, DC conductivity, and Grüneisen parameter have been made. These measurements allowed the origins of the contrast provided by each substance to be identified. Methods: The electric and magnetic properties of the contrast agents were characterized at 3 GHz using two rectangular waveguide cavities. The DC conductivity was measured separately using a conductivity meter. Thermoacoustic signals were then acquired and compared to those generated in water. Finally, 3D electromagnetic simulations were used to decouple the different contributions to the absorbed power density. Results: It was found that the gadolinium compounds provided appreciable electric contrast but not originating from the gadolinium itself. The contrast was either due to dissociation of the gadolinium salt which increased ionic conductivity or its nondissociated polar fraction which increased dielectric polarization loss or a combination of both. In addition, very high concentrations were required to achieve appreciable contrast, to the extent that the Grüneisen parameter increased significantly and became a source of contrast. Iron oxide particles were found to produce low but measurable dielectric contrast due to dielectric polarization loss, but this is attributed to the coating of the particles not the iron oxide. Single wall carbon nanotubes did not provide measurable contrast of any type

  17. [Investigation of the presence of Cryptosporidium spp. in different water sources in Mersin province, Turkey].

    Science.gov (United States)

    Aslan, Gönül; Bayram, Gül; Otağ, Feza; Direkel, Sahin; Taylan Özkan, Ayşegül; Ceber, Kemal; Emekdaş, Gürol

    2012-01-01

    Cryptosporidium is an intracellular protozoon that causes enteritis in human and animals. Contaminated water and food are the major sources for the transmission of oocysts via oral-fecal route. It is reported that the prevalence of cryptosporidiosis is higher in developing countries than developed countries because of inefficient sanitation and disinfection facilities for drinking water. The most frequently detected species is Cryptosporidium parvum leading to high morbidity in healthy subjects and also fatal infections in immunocompromised patients. The acid-fast staining method is widely used in the diagnosis of cryptosporidiosis. Nowadays, Cryptosporidium could easily be detected in water supplies and asymptomatic carriers by molecular techniques to obtain epidemiological data. In this study it was aimed to detect and identify Cryptosporidium oocysts in different water sources in Mersin province, Turkey. A total of 135 water samples (70 taps, 50 wells and 15 sewage) collected from city center (n= 25) and from Tarsus (n= 32), Mezitli (n= 33) and Karaduvar (n= 45) counties between March 2007 and May 2009 were included in the study. Water samples in 10 liter volumes, were filtered by 0.45 µm pore-sized membrane filter vacuum/ pressure pumping technique. Cryptosporidium oocysts in filtrates were detected by modified cold Kinyoun acid-fast stain (MCK) technique and also identified and typed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. MCK yielded three and PCR yielded seven positive results. All the strains were identified as C.parvum by PCR-RFLP method. All of the three MCK-positive samples were also found positive with PCR, however four PCR positive samples were MCK-negative. Thus, the prevalence of C.parvum was estimated as 5.2% (7/135) in our region. Of seven positive samples, one was a sewage water sample collected from the city center, while the remaining (two tap water, two well water and two sewage water samples

  18. Bacterial prostatitis.

    Science.gov (United States)

    Gill, Bradley C; Shoskes, Daniel A

    2016-02-01

    The review provides the infectious disease community with a urologic perspective on bacterial prostatitis. Specifically, the article briefly reviews the categorization of prostatitis by type and provides a distillation of new findings published on bacterial prostatitis over the past year. It also highlights key points from the established literature. Cross-sectional prostate imaging is becoming more common and may lead to more incidental diagnoses of acute bacterial prostatitis. As drug resistance remains problematic in this condition, the reemergence of older antibiotics such as fosfomycin, has proven beneficial. With regard to chronic bacterial prostatitis, no clear clinical risk factors emerged in a large epidemiological study. However, bacterial biofilm formation has been associated with more severe cases. Surgery has a limited role in bacterial prostatitis and should be reserved for draining of a prostatic abscess or the removal of infected prostatic stones. Prostatitis remains a common and bothersome clinical condition. Antibiotic therapy remains the basis of treatment for both acute and chronic bacterial prostatitis. Further research into improving prostatitis treatment is indicated.

  19. Source evaluation report phase 2 investigation: Limited field investigation. Final report: United States Air Force Environmental Restoration Program, Eielson Air Force Base, Alaska

    Energy Technology Data Exchange (ETDEWEB)

    1994-10-01

    This report describes the limited field investigation work done to address issues and answer unresolved questions regarding a collection of potential contaminant sources at Eielson Air Force Base (AFB), near Fairbanks, Alaska. These sources were listed in the Eielson AFB Federal Facility Agreement supporting the Comprehensive Environmental Response, Compensation, and Liability Act (CERCLA) cleanup of the base. The limited field investigation began in 1993 to resolve all remaining technical issues and provide the data and analysis required to evaluate the environmental hazard associated with these sites. The objective of the limited field investigation was to allow the remedial project managers to sort each site into one of three categories: requiring remedial investigation/feasibility study, requiring interim removal action, or requiring no further remedial action.

  20. Energetic and exergetic investigation of an organic Rankine cycle at different heat source temperatures

    International Nuclear Information System (INIS)

    Li, Jing; Pei, Gang; Li, Yunzhu; Wang, Dongyue; Ji, Jie

    2012-01-01

    The energetic and exergetic performance of an updated ORC (organic Rankine cycle) is investigated. The thermal efficiencies of the ORC at different heat source temperatures of about 100, 90, 80, and 70 °C are explored. The thermodynamic irreversibility that takes place in the evaporator, condenser, turbine, pump, and separator is revealed. The ORC feasibility for low-temperature applications is demonstrated. With a hot side temperature of around 80 °C, a thermal efficiency of 7.4% and a turbine isentropic efficiency of 0.68 can be achieved. The present research further indicates that exergy destruction caused by heat transfer through an appreciable temperature difference in the evaporator is the largest in the energy conversion process, followed by that in the condenser. The exergy destroyed in the heat exchangers amounts to 74% of the overall exergy loss. The total system exergy efficiency is approximately 40%; thus, ways to improve exergy efficiency are required. HCFC-123, a dry fluid, is experimentally confirmed to be highly superheated after expansion in this study. A regenerator should be used to preheat HCFC-123 prior to entering the evaporator. Meanwhile the heat-transfer configuration with two oil cycles can be a good solution to overcome the thermodynamic disadvantage of a one-stage evaporator. -- Highlights: ► An updated ORC system is introduced. ► The ORC feasibility for low-temperature applications is experimentally demonstrated. ► Thermodynamic irreversibility in ORC components is revealed. ► Suggestions are given to reduce the exergy destruction.

  1. The Visual Code Navigator : An Interactive Toolset for Source Code Investigation

    NARCIS (Netherlands)

    Lommerse, Gerard; Nossin, Freek; Voinea, Lucian; Telea, Alexandru

    2005-01-01

    We present the Visual Code Navigator, a set of three interrelated visual tools that we developed for exploring large source code software projects from three different perspectives, or views: The syntactic view shows the syntactic constructs in the source code. The symbol view shows the objects a

  2. Sources of the Geomagnetic Field and theModern Data That Enable Their Investigation

    DEFF Research Database (Denmark)

    Olsen, Nils; Hulot, Gauthier; Sabaka, Terence J.

    2010-01-01

    Abstract The geomagnetic field one canmeasure at the Earth’s surface or on board satellites is the sumof contributions frommany different sources.These sources have different physical origins and can be found both below (in the form of electrical currents and magnetized material) and above (only...

  3. Bacterial Vaginosis

    Science.gov (United States)

    ... Archive STDs Home Page Bacterial Vaginosis (BV) Chlamydia Gonorrhea Genital Herpes Hepatitis HIV/AIDS & STDs Human Papillomavirus ( ... of getting other STDs, such as chlamydia and gonorrhea . These bacteria can sometimes cause pelvic inflammatory disease ( ...

  4. Remembering the color of objects: an ERP investigation of source memory.

    Science.gov (United States)

    Cycowicz, Y M; Friedman, D; Snodgrass, J G

    2001-04-01

    Subjects studied pictures of common objects outlined in either red or green and were asked to memorize the objects and their associated colors. Event-related potentials (ERPs) were recorded during subsequent inclusion (i.e. item) and exclusion (i.e. source) memory tasks. The main goal of the experiment was to determine if brain signatures for familiarity and recollection, two behavioral processes thought to account for episodic memory performance, would be observed in the pattern of ERP results. For correctly recognized items, early, posterior old/new effects were recorded (approximately 300--600 ms) that did not differ in magnitude or scalp distribution between item and source memory tasks. A subsequent long-duration occipitally focused negativity (approximately 800 ms peak) was evident in the source but not the item memory task. The ERPs associated with 'source errors' in the source memory task also showed robust early old/new effects. However, 'source error' ERPs lacked frontal scalp activity compared to those associated with correct source attribution. The data suggest that a recollective response may require frontal involvement whereas a decision based on familiarity may not.

  5. Supply risk management functions of sourcing intermediaries – an investigation of the clothing industry

    DEFF Research Database (Denmark)

    Vedel, Mette; Ellegaard, Chris

    2013-01-01

    Purpose: The purpose of this research is to uncover how buying companies use sourcing intermediaries to manage supply risks in global sourcing. Design/methodology/approach: We carry out an explorative qualitative study of the clothing industry, interviewing key respondents that occupy different...... intermediary types, characterised by the set of functions they handle. Research limitations/implications: By analysing a limited set of in-depth interviews in one industry we have traded off broader analytical generalization for in-depth exploration and theory building. Therefore, future research should test...... by identifying the supply risk management functions that sourcing intermediaries carry out for buying companies. We also contribute by uncovering different types of sourcing intermediaries, determined by the collection of functions handled....

  6. Investigation of Near-Field Electromagnetic Source Imaging Using Inverse Green's Function Integrations

    National Research Council Canada - National Science Library

    Steenman, Daryl

    1999-01-01

    .... In the far-field of these tested objects, actual sources of high reflectivity or "Hot Spots" on the tested objects can be isolated to within only one half the wavelength of the electromagnetic wave used for testing...

  7. Source terms: an investigation of uncertainties, magnitudes, and recommendations for research. [PWR; BWR

    Energy Technology Data Exchange (ETDEWEB)

    Levine, S.; Kaiser, G. D.; Arcieri, W. C.; Firstenberg, H.; Fulford, P. J.; Lam, P. S.; Ritzman, R. L.; Schmidt, E. R.

    1982-03-01

    The purpose of this document is to assess the state of knowledge and expert opinions that exist about fission product source terms from potential nuclear power plant accidents. This is so that recommendations can be made for research and analyses which have the potential to reduce the uncertainties in these estimated source terms and to derive improved methods for predicting their magnitudes. The main reasons for writing this report are to indicate the major uncertainties involved in defining realistic source terms that could arise from severe reactor accidents, to determine which factors would have the most significant impact on public risks and emergency planning, and to suggest research and analyses that could result in the reduction of these uncertainties. Source terms used in the conventional consequence calculations in the licensing process are not explicitly addressed.

  8. Longitudinal investigation of source memory reveals different developmental trajectories for item memory and binding

    OpenAIRE

    Riggins, Tracy

    2013-01-01

    The present study used a cohort-sequential design to examine developmental changes in children's ability to bind items in memory during early and middle childhood. Three cohorts of children (aged 4, 6, or 8 years) were followed longitudinally for three years. Each year, children completed a source memory paradigm assessing memory for items and binding. Results suggest linear increases in memory for individual items (facts or sources) between 4 and 10 years of age, but that memory for correct ...

  9. Investigating the feasibility of geo-Tagged photographs as sources of land cover input data

    OpenAIRE

    Antoniou, Vyron; Fonte, Cidália Costa; See, Linda; Estima, Jacinto; Arsanjani, Jamal Jokar; Lupia, Flavio; Minghini, Marco; Foody, Giles; Fritz, Steffen

    2016-01-01

    Geo-Tagged photographs are used increasingly as a source of Volunteered Geographic Information (VGI), which could potentially be used for land use and land cover applications. The purpose of this paper is to analyze the feasibility of using this source of spatial information for three use cases related to land cover: Calibration, validation and verification. We first provide an inventory of the metadata that are collected with geo-Tagged photographs and then consider what elements would be es...

  10. Data compilation task report for the source investigation of the 300-FF-1 operable unit phase 1 remedial investigation

    International Nuclear Information System (INIS)

    Young, J.S.; Fruland, R.M.; Fruchter, J.S.

    1990-02-01

    This report provides additional information on facility and waste characteristics for the 300-FF-1 operable unit. The additional information gathered and reported includes meetings and on-site visits with current and past personnel having knowledge of operations in the operable unit, a more precise determination of the location of the Process Sewer lines and Retired Radioactive Liquid Waste Sewer, a better understanding of the phosphoric acid spill at the 340 Complex, and a search for engineering plans and environmental reports related to the operable unit. As a result of this data-gathering effort, recommendations for further investigation include characterization of the 307 Trenches to determine the origin of an underlying uranium plume in the groundwater, more extensive sampling of near-surface and dike sediments in the North and South Process Ponds to better define the extent of horizontal contamination, and detection of possible leaks in the abandoned Radioactive Waste Sewer by either electromagnetic induction or remote television camera inspection techniques. 16 refs., 4 figs., 5 tabs

  11. Investigating the effects of point source and nonpoint source pollution on the water quality of the East River (Dongjiang) in South China

    Science.gov (United States)

    Wu, Yiping; Chen, Ji

    2013-01-01

    Understanding the physical processes of point source (PS) and nonpoint source (NPS) pollution is critical to evaluate river water quality and identify major pollutant sources in a watershed. In this study, we used the physically-based hydrological/water quality model, Soil and Water Assessment Tool, to investigate the influence of PS and NPS pollution on the water quality of the East River (Dongjiang in Chinese) in southern China. Our results indicate that NPS pollution was the dominant contribution (>94%) to nutrient loads except for mineral phosphorus (50%). A comprehensive Water Quality Index (WQI) computed using eight key water quality variables demonstrates that water quality is better upstream than downstream despite the higher level of ammonium nitrogen found in upstream waters. Also, the temporal (seasonal) and spatial distributions of nutrient loads clearly indicate the critical time period (from late dry season to early wet season) and pollution source areas within the basin (middle and downstream agricultural lands), which resource managers can use to accomplish substantial reduction of NPS pollutant loadings. Overall, this study helps our understanding of the relationship between human activities and pollutant loads and further contributes to decision support for local watershed managers to protect water quality in this region. In particular, the methods presented such as integrating WQI with watershed modeling and identifying the critical time period and pollutions source areas can be valuable for other researchers worldwide.

  12. [Experimental investigation of laser plasma soft X-ray source with gas target].

    Science.gov (United States)

    Ni, Qi-liang; Gong, Yan; Lin, Jing-quan; Chen, Bo; Cao, Jian-lin

    2003-02-01

    This paper describes a debris-free laser plasma soft X-ray source with a gas target, which has high operating frequency and can produce strong soft X-ray radiation. The valve of this light source is drived by a piezoelectrical ceramic whose operating frequency is up to 400 Hz. In comparison with laser plasma soft X-ray sources using metal target, the light source is debris-free. And it has higher operating frequency than gas target soft X-ray sources whose nozzle is controlled by a solenoid valve. A channel electron multiplier (CEM) operating in analog mode is used to detect the soft X-ray generated by the laser plasma source, and the CEM's output is fed to to a charge-sensitive preamplifier for further amplification purpose. Output charges from the CEM are proportional to the amplitude of the preamplifier's output voltage. Spectra of CO2, Xe and Kr at 8-14 nm wavelength which can be used for soft X-ray projection lithography are measured. The spectrum for CO2 consists of separate spectral lines originate mainly from the transitions in Li-like and Be-like ions. The Xe spectrum originating mainly from 4d-5f, 4d-4f, 4d-6p and 4d-5p transitions in multiply charged xenon ions. The spectrum for Kr consists of separate spectral lines and continuous broad spectra originating mainly from the transitions in Cu-, Ni-, Co- and Fe-like ions.

  13. Investigation of the effective components of the flowers of Trollius chinensis from the perspectives of intestinal bacterial transformation and intestinal absorption.

    Science.gov (United States)

    Guo, Lina; Qiao, Shanshan; Hu, Junhong; Li, Deli; Zheng, Shiqi; Shi, Duozhi; Liu, Junxiu; Wang, Rufeng

    2017-12-01

    The flowers of Trollius chinensis Bunge (Ranunculaceae), used for respiratory tract infections, mainly contain flavonoids, phenolic acids, and alkaloids; however, the effective components are debatable because of their unclear in vivo activities. This study investigates the effective components from the perspectives of biotransformation and absorption. Both single person derived- and multiple people-derived intestinal florae were used to investigate the biotransformation of aqueous extract of the flowers of T. chinensis (AEOF) at the concentrations of 15.0, 30.0, and 60.0 mg/mL, respectively, for 72 h. Both human colon adenocarcinoma cell line (Caco-2) monolayers and everted gut sacs were employed to evaluate the intestinal absorption of the intestinal bacterial transformed AEOF at the concentrations of 10, 20, and 30 mg/mL, respectively, for 180 min. 2″-O-β-l-Galactopyranosylorientin, orientin, vitexin, quercetin, veratric acid, proglobeflowery acid, and trolline in AEOF were not transformed by intestinal bacteria, while isoquercetin and trollioside were completely transformed. The P app values of 2″-O-β-l-galactopyranosylorientin, orientin, and vitexin calculated based on the experimental data of intestinal absorption were at the levels of 10 -5 , whereas those of veratric acid, proglobeflowery acid, and trolline were at 10 -4 . The mass ratio of flavonoids to phenolic acids to alkaloids changed from 16:10:7 to 9:12:8 before and after absorption. The dominant position of flavonoids was replaced by phenolic acids after absorption. In addition to flavonoids which are usually considered as the dominant effective ones, phenolic acids and alkaloids should be also very important for the efficacy of these flowers.

  14. Moderators of Framing Effects on Political Attitudes: Is Source Credibility Worth Investigating?

    Directory of Open Access Journals (Sweden)

    Dana Raluca Buturoiu

    2015-08-01

    Full Text Available This research paper focuses on indirect (mediated media effects. In particular, we discuss which independent variables might intervene in and moderate the impact of framing effects on public attitudes (namely political trust, both in short-term and medium-term contexts. Among these, we focus on source credibility as a possible moderator of framing effects over time. The purpose of this study was to examine if and how source credibility influences individuals’ political trust. The moderator role of source credibility is analysed according to the exposure to different types of frames (repetitive or competitive at different moments (one week or one month. By means of a framing experiment (N=769 on political topics, we argue that media frames could influence political trust: Source credibility has a marginal influence, which suggests that, with stronger stimulus material (video, as opposed to written press articles, the source could play an important role in the willingness of people to trust political figures in general. Thus, we might argue that the media play a significant role not only in offering information about politics and politicians, but also in altering people’s perceptions about them. On the other hand, time seems to matter, since framing effects are more powerful after competitive media exposures. This study proposes new theoretical insights into framing effects, in the sense that classical theories should be revisited in various cultural or political contexts

  15. Nonpolar organic compounds as PM2.5 source tracers: Investigation of their sources and degradation in the Pearl River Delta, China

    Science.gov (United States)

    Wang, Qiongqiong; Feng, Yongming; Huang, X. H. Hilda; Griffith, Stephen M.; Zhang, Ting; Zhang, Qingyan; Wu, Dui; Yu, Jian Zhen

    2016-10-01

    A group of nonpolar organic compounds (NPOCs) in five compound classes including alkanes, polycyclic aromatic hydrocarbons (PAHs), hopanes, steranes, and 1,3,5-triphenylbenzene were quantified in samples of particulate matter of aerodynamic diameter less than 2.5 μm collected at four sites in the Pearl River Delta (PRD) region, China, over a 2 year period from 2011 to 2012. The four sites include industrial (Nanhai), urban (Guangzhou), urban outskirt (Dongguan), and suburban (Nansha) locations. Some NPOCs are uniquely emitted from particular combustion sources and thereby serving as markers in source apportionment. Based on this multiyear and multisite NPOC data set, spatial and seasonal variations, correlation analysis, and ratio-ratio plots were used to investigate the source information and degradation of NPOC tracers. In summer, NPOCs showed distinct local emission characteristics, with urban sites having much higher concentrations than suburban sites. In winter, regional transport was an important influence on NPOC levels, driving up concentrations at all sampling sites and diminishing an urban-suburban spatial gradient. The lighter NPOCs exhibited more prominent seasonal variations. Such spatiotemporal features suggest that their particle-phase abundance is more influenced by temperature, which is a critical factor in controlling the extent of semivolatile organics partitioned into the aerosol phase. The heavier NPOCs, especially PAHs, showed negligible correlation among the four sites, suggesting more influence from local emissions. Ratio-ratio plots indicate photodegradation and mixing of various sources for the NPOCs in the PRD. A positive matrix factorization (PMF) analysis of this large NPOC data set suggests that heavier NPOCs are more suitable source indicators than lighter NPOCs. Incorporating particle-phase light NPOC concentrations in PMF produces a separate factor, which primarily contains those light NPOCs and likely is not a source factor. Total

  16. Non-polar organic compounds as PM2.5 source tracers: Investigation of their sources and degradation in the Pearl River Delta, China

    Science.gov (United States)

    Wang, Q.; Feng, Y.; Huang, X. H. H.; Griffith, S.; Zhang, T.; Zhang, Q.; Wu, D.; Yu, J.

    2016-12-01

    Nonpolar organic compounds (NPOCs) including alkanes, polycyclic aromatic hydrocarbons (PAHs), hopanes, steranes, and 1,3,5-triphenylbenzene, were quantified in PM2.5 samples at four sites in the Pearl River Delta (PRD) region, China over a two-year period from 2011 to 2012. The four sites include one industrial zone (Nanhai), one urban (Guangzhou), one urban outskirt (Dongguan) and one suburban (Nansha) locations. Some NPOCs are uniquely emitted from particular combustion sources, and thereby serving as convenient markers in source apportionment. Based on this multi-year and multi-site data set, spatial and seasonal variations, correlation analysis and ratio-ratio plots were used to investigate the source information and degradation of NPOC tracers. In summer, NPOCs showed distinct local emission characteristics, with urban sites having much higher concentrations than suburban site. In winter, regional transport was an important influence on NPOC levels, driving up concentrations at all sampling sites and diminishing an urban-suburban spatial gradient. The lighter NPOCs exhibited more prominent seasonal variations, suggesting their particle-phase abundance is more influenced by temperature, a critical factor in controlling the extent of semi-volatile organics partitioned into the aerosol phase. The heavier NPOCs, especially PAHs, showed negligible correlation among the four sites, suggesting more influence from local emissions. Ratio-ratio plots indicate photo-degradation and mixing of various sources for the NPOCs in the PRD. A positive matrix factorization (PMF) analysis of this large NPOC data set suggests that heavier NPOCs are more suitable source indicators than lighter NPOCs. Incorporating particle-phase light NPOC concentrations in PMF produces a separate factor, which primarily contains those light NPOCs and likely is not a source factor. Total NPOC concentrations predicted using Pankow partitioning theory were explored as PMF inputs, however, the PMF

  17. Opportunities of Gallium Sage experiment with artificial neutrino sources for investigation of neutrino to sterile states

    International Nuclear Information System (INIS)

    Gavrin, V.N.; Gorbachiev, V.V.; Veretenkin, E.P.

    2011-01-01

    The unexpectedly low capture rate of neutrino in Ga source experiments in SAGE and GALLEX can be explained assuming electron neutrino transitions to sterile states with a mass-squared difference ∼ 1eV 2 . To test this oscillation hypothesis, we propose to place a very intense 51 Cr source at the center of a 50 tonne target of gallium metal that is divided into two zones and to measure the neutrino capture rate in each zone. The Experiment has the potential to test neutrino oscillation transitions with mass-squared difference Δm 2 > 0.5 eV 2 . An optimized SAGE setup and 3 MCi source of 51 Cr would provide a sensitivity to electron neutrino disappearance of a few percent.

  18. Investigation of source position uncertainties & balloon deformation in MammoSite brachytherapy on treatment effectiveness

    International Nuclear Information System (INIS)

    Bensaleh, S.

    2010-01-01

    The MammoSite ® breast high dose rate brachytherapy is used in treatment of early-stage breast cancer. The tumour bed volume is irradiated with high dose per fraction in a relatively small number of fractions. Uncertainties in the source positioning and MammoSite balloon deformation will alter the prescribed dose within the treated volume. They may also expose the normal tissues in balloon proximity to excessive dose. The purpose of this work is to explore the impact of these two uncertainties on the MammoSite dose distribution in the breast using dose volume histograms and Monte Carlo simulations. The Lyman–Kutcher and relative seriality models were employed to estimate the normal tissues complications associated with the MammoSite dose distributions. The tumour control probability was calculated using the Poisson model. This study gives low probabilities for developing heart and lung complications. The probability of complications of the skin and normal breast tissues depends on the location of the source inside the balloon and the volume receiving high dose. Incorrect source position and balloon deformation had significant effect on the prescribed dose within the treated volume. A 4 mm balloon deformation resulted in reduction of the tumour control probability by 24%. Monte Carlo calculations using EGSnrc showed that a deviation of the source by 1 mm caused approximately 7% dose reduction in the treated target volume at 1 cm from the balloon surface. In conclusion, accurate positioning of the 192 Ir source at the balloon centre and minimal balloon deformation are critical for proper dose delivery with the MammoSite brachytherapy applicator. On the basis of this study, we suggest that the MammoSite treatment protocols should allow for a balloon deformation of ≤2 mm and a maximum source deviation of ≤1 mm.

  19. An Investigation of Customer Accounting Systems as a Source of Sustainable Competitive Advantage

    DEFF Research Database (Denmark)

    Holm, Morten; Kumar, V.; Plenborg, Thomas

    2016-01-01

    This study examines whether using customer accounting systems for resource allocation purposes is a source of sustainable competitive advantage. Based on a longitudinal data set comparing the performance of firms that adopt customer accounting and their industry benchmarks, we find that financial...... accounting systems is a source of temporary rather than sustainable competitive advantage. The results are robust to other strategic events around the time of adoption, different matching of peers, and the influence of other factors that could be expected to influence firm performance. We discuss...

  20. Investigation of high-energy sources in optical light by ESA Gaia

    Czech Academy of Sciences Publication Activity Database

    Hudec, R.; Šimon, Vojtěch; Hudec, L.; Hudcová, Věra

    2010-01-01

    Roč. 81, č. 1 (2010), s. 476-481 ISSN 0037-8720. [Multifrequency behaviour of high energy cosmic sources. Vulcano, 25.05.2009-30.05. 2009] R&D Projects: GA ČR GA205/08/1207 Grant - others:ESA(XE) ESA- PECS project No. 98058; GA ČR(CZ) GA102/09/0997; GA MŠk(CZ) ME09027; ESA(XE) ESA- PECS project No. 98023 Institutional research plan: CEZ:AV0Z10030501 Keywords : high-energy sources * cataclysmic variables * low-dispersion spectra Subject RIV: BN - Astronomy, Celestial Mechanics, Astrophysics

  1. Investigation of the sources and fate of radioactive discharges to public sewers

    International Nuclear Information System (INIS)

    Titley, J.; Carey, A.; Crockett, G.

    2000-01-01

    The practise of disposing of small amounts of liquid radioactive waste from non-nuclear organisations to public sewer systems, is currently being reviewed by the Environment Agency, Scottish Environmental Protection Agency and the Industrial Pollution and Radiochemical Inspectorate. Changing sewage treatment and disposal practises, particularly the increasing use of incineration to treat sewage sludge in urban areas, have prompted this review. This study was undertaken as part of the review process to determine whether disposal into the public sewer system remains a safe disposal option. This report describes a study to investigate the sources and fates of authorised discharges of radionuclides into the sewer system. Sewage transport, treatment, discharge and incineration systems are described and the fate of radionuclides in the systems considered. A model (SMART) was developed to predict radionuclide behaviour through these systems and calculate the resulting doses. Case studies of two large urban sewerage systems were carried out in Leeds and Central London. Leeds is served by one sewage treatment works at Knostrop, which receives disposals of ten radionuclides from six disposers. Up to seven of the radionuclides were measurable in effluents, sludges or incinerator ash at the works. Naturally occurring and fallout radionuclides were also detected. Central London is served by one large works at Beckton which receives disposals of twenty radionuclides from 65 disposers. Up to ten of the disposed radionuclides, plus naturally occurring and fallout radionuclides were detected in effluents, sludges, incinerator cake or ash at Beckton. For both sites the largest authorised disposals were of Tc-99m, C-14, I-125, I-131 and H-3. The behaviour of radionuclides during incineration was assessed using measurements and literature values. The radiological implications of disposals in Leeds and Central London were assessed using the model SMART and by measuring dose rates

  2. Biodegradability of bacterial surfactants.

    Science.gov (United States)

    Lima, Tânia M S; Procópio, Lorena C; Brandão, Felipe D; Carvalho, André M X; Tótola, Marcos R; Borges, Arnaldo C

    2011-06-01

    This work aimed at evaluating the biodegradability of different bacterial surfactants in liquid medium and in soil microcosms. The biodegradability of biosurfactants by pure and mixed bacterial cultures was evaluated through CO(2) evolution. Three bacterial strains, Acinetobacter baumanni LBBMA ES11, Acinetobacter haemolyticus LBBMA 53 and Pseudomonas sp. LBBMA 101B, used the biosurfactants produced by Bacillus sp. LBBMA 111A (mixed lipopeptide), Bacillus subtilis LBBMA 155 (lipopeptide), Flavobacterium sp. LBBMA 168 (mixture of flavolipids), Dietzia Maris LBBMA 191(glycolipid) and Arthrobacter oxydans LBBMA 201(lipopeptide) as carbon sources in minimal medium. The synthetic surfactant sodium dodecyl sulfate (SDS) was also mineralized by these microorganisms, but at a lower rate. CO(2) emitted by a mixed bacterial culture in soil microcosms with biosurfactants was higher than in the microcosm containing SDS. Biosurfactant mineralization in soil was confirmed by the increase in surface tension of the soil aqueous extracts after incubation with the mixed bacterial culture. It can be concluded that, in terms of biodegradability and environmental security, these compounds are more suitable for applications in remediation technologies in comparison to synthetic surfactants. However, more information is needed on structure of biosurfactants, their interaction with soil and contaminants and scale up and cost for biosurfactant production.

  3. Longitudinal Investigation of Source Memory Reveals Different Developmental Trajectories for Item Memory and Binding

    Science.gov (United States)

    Riggins, Tracy

    2014-01-01

    The present study used a cohort-sequential design to examine developmental changes in children's ability to bind items in memory during early and middle childhood. Three cohorts of children (aged 4, 6, or 8 years) were followed longitudinally for 3 years. Each year, children completed a source memory paradigm assessing memory for items and…

  4. An investigation of the atmospheric sources and sinks of methyl bromide

    International Nuclear Information System (INIS)

    Singh, H.B.; Kanakidou, M.

    1993-01-01

    Methyl Bromide (CH 3 Br) is a ubiquitous component of the atmosphere and has been implicated as an important player in the depletion of stratospheric ozone. Atmospheric CH 3 Br abundances, interhemispheric gradients, oceanic concentrations, man-made emissions, and removal processes have been analyzed and interpreted with the help of a simple box model and a 2-D global photochemical model. Its calculated atmospheric lifetime (T) of 1.7-1.9 years, based on reaction with OH radicals, is consistent with a global source of 90-110 Gg (10 9 g)/year. Consequences of a much shorter lifetime of 1.2 years, due to possible deposition/hydrolysis losses, are also considered. Available data are used to estimate a CH 3 Br source that is 35% (20-50%) man-made and 65% (80-50%) natural. Oceans are substantially supersaturated and provide the most important natural source of ∼60 (40-80) Gg/year. Within the oceans 200-300 Gg/year of CH 3 Br may be produced. Indirect emissions from automobile exhaust and biomass burning can not be well quantified (1-10 Gg/year). A global trend of 0.1-0.2 ppt/year is predicted. Model results show significant vertical and seasonal variations in the atmospheric abundances and interhemispheric gradients of CH 3 Br. Substantial uncertainties in calibrations, source estimates, and deposition processes are present. 12 refs., 3 figs., 2 tabs

  5. The analytical investigation of the super-Gaussian pump source on ...

    Indian Academy of Sciences (India)

    In this paper, we assumed that the fiber core and first clad are exposed to a pump source with a super-Gaussian profile of order four. The effects of this non-uniform heat deposition on thermal, stress and thermo-optics properties such as temperature-dependent change of refractive index and thermally induced stress have ...

  6. An Experimental Investigation of News Source and the Hostile Media Effect.

    Science.gov (United States)

    Arpan, Laura M.; Raney, Arthur A.

    2003-01-01

    Examines the interaction among different news sources, individual levels of partisanship, and the hostile media effect in sports news. Explains that university students read a balanced story about their home-town college football team in one of three newspapers: the home-town, the cross-state rival university's town, or a neutral town paper.…

  7. Sources of Stress among Parents of Children with Intellectual Disabilities: A Preliminary Investigation in Saudi Arabia

    Science.gov (United States)

    Aldosari, Mubarak S.; Pufpaff, Lisa A.

    2014-01-01

    This study identified differences in sources of stress between parents of male children with intellectual disabilities in Saudi Arabia. Seventeen pairs of parents completed the Parent Stress Index (Abidin, 1995). Each pair of parents had a male child diagnosed with intellectual disability who either attended an institute for male children with…

  8. 76 FR 1173 - Draft Guidance for Industry on Electronic Source Documentation in Clinical Investigations...

    Science.gov (United States)

    2011-01-07

    ... Web page at http://www.fda.gov/RegulatoryInformation/Guidances/default.htm . FDA guidances are issued and updated regularly. We recommend you check the Web site to ensure that you have the most up-to-date... electronic diaries provided by study subjects. When paper source documents are available for review, tracing...

  9. BACTERIAL CONSORTIUM

    Directory of Open Access Journals (Sweden)

    Payel Sarkar

    2013-01-01

    Full Text Available Petroleum aromatic hydrocarbons like benzen e, toluene, ethyl benzene and xylene, together known as BTEX, has almost the same chemical structure. These aromatic hydrocarbons are released as pollutants in th e environment. This work was taken up to develop a solvent tolerant bacterial cons ortium that could degrade BTEX compounds as they all share a common chemical structure. We have isolated almost 60 different types of bacterial strains from different petroleum contaminated sites. Of these 60 bacterial strains almost 20 microorganisms were screene d on the basis of capability to tolerate high concentration of BTEX. Ten differe nt consortia were prepared and the compatibility of the bacterial strains within the consortia was checked by gram staining and BTEX tolerance level. Four successful mi crobial consortia were selected in which all the bacterial strains concomitantly grew in presence of high concentration of BTEX (10% of toluene, 10% of benzene 5% ethyl benzene and 1% xylene. Consortium #2 showed the highest growth rate in pr esence of BTEX. Degradation of BTEX by consortium #2 was monitored for 5 days by gradual decrease in the volume of the solvents. The maximum reduction observed wa s 85% in 5 days. Gas chromatography results also reveal that could completely degrade benzene and ethyl benzene within 48 hours. Almost 90% degradation of toluene and xylene in 48 hours was exhibited by consortium #2. It could also tolerate and degrade many industrial solvents such as chloroform, DMSO, acetonitrile having a wide range of log P values (0.03–3.1. Degradation of aromatic hydrocarbon like BTEX by a solvent tolerant bacterial consortium is greatly significant as it could degrade high concentration of pollutants compared to a bacterium and also reduces the time span of degradation.

  10. Investigation on the MOC with a linear source approximation scheme in three-dimensional assembly

    International Nuclear Information System (INIS)

    Zhu, Chenglin; Cao, Xinrong

    2014-01-01

    Method of characteristics (MOC) for solving neutron transport equation has already become one of the fundamental methods for lattice calculation of nuclear design code system. At present, MOC has three schemes to deal with the neutron source of the transport equation: the flat source approximation of the step characteristics (SC) scheme, the diamond difference (DD) scheme and the linear source (LS) characteristics scheme. The MOC for SC scheme and DD scheme need large storage space and long computing time when they are used to calculate large-scale three-dimensional neutron transport problems. In this paper, a LS scheme and its correction for negative source distribution were developed and added to DRAGON code. This new scheme was compared with the SC scheme and DD scheme which had been applied in this code. As an open source code, DRAGON could solve three-dimensional assembly with MOC method. Detailed calculation is conducted on two-dimensional VVER-1000 assembly under three schemes of MOC. The numerical results indicate that coarse mesh could be used in the LS scheme with the same accuracy. And the LS scheme applied in DRAGON is effective and expected results are achieved. Then three-dimensional cell problem and VVER-1000 assembly are calculated with LS scheme and SC scheme. The results show that less memory and shorter computational time are employed in LS scheme compared with SC scheme. It is concluded that by using LS scheme, DRAGON is able to calculate large-scale three-dimensional problems with less storage space and shorter computing time

  11. Marine and land active-source seismic investigation of geothermal potential, tectonic structure, and earthquake hazards in Pyramid Lake, Nevada

    Energy Technology Data Exchange (ETDEWEB)

    Eisses, A.; Kell, A.; Kent, G. [UNR; Driscoll, N. [UCSD; Karlin, R.; Baskin, R. [USGS; Louie, J. [UNR; Pullammanappallil, S. [Optim

    2016-08-01

    Amy Eisses, Annie M. Kell, Graham Kent, Neal W. Driscoll, Robert E. Karlin, Robert L. Baskin, John N. Louie, Kenneth D. Smith, Sathish Pullammanappallil, 2011, Marine and land active-source seismic investigation of geothermal potential, tectonic structure, and earthquake hazards in Pyramid Lake, Nevada: presented at American Geophysical Union Fall Meeting, San Francisco, Dec. 5-9, abstract NS14A-08.

  12. Spectral investigation of neutron radiation in three-sectional concrete labyrinth from a californium-252 source

    International Nuclear Information System (INIS)

    Belogorlov, E.A.; Britvich, G.I.; Getmanov, V.B.

    1985-01-01

    Construction of labyrinths in points of communication output from the storage-ring under construction is accompanied by numerous difficulties due to a considerable number of gas and cryogenic pipelines, which require large cross sections at the minimal length of the pipelines proper for their location. It results in unfavourable for radiation attenuation ratios between cross section and length of the labyrinth separate sections. Neutron spectra in a model concrete labyrinth, at the entrance to which a neutron source with fission spectrum (californium-252) and the same source in a polyethylene moderator are located, are measured. On the basis of the spectra obtained the formation of fluence and equivalent dose along the labyrinth geometric axis is analyzed. Conditions permitting actually to reduce radiation dose in the labyrinth (dead end provision, the use of cover materials, construction of diaphragms and shielding plates) are simulated

  13. Creation and investigation of powerful EUV sources (λ ∼ 13.5 nm)

    International Nuclear Information System (INIS)

    Borisov, V. M.; Borisova, G. N.; Vinokhodov, A. Yu.; Ivanov, A. S.; Kiryukhin, Yu. B.; Mishchenko, V. A.; Prokofiev, A. V.; Khristoforov, O. B.

    2010-01-01

    Results are presented from experimental studies of repetitively pulsed EUV (λ = 13.5 ± 0.135 nm) sources based on a laser-initiated discharge in tin vapor between rotating disk electrodes. Radiative characteristics of two sources with different systems of tin supply onto the electrode surface and different types of power supply have been compared. A number of new effects have been revealed at pulse repetition rates as high as ∼4000 Hz. A mean radiation power of 520 W into the 2π solid angle has been achieved in the spectral band 13.5 ± 0.135 nm at a deposited electrical power of 24 kW.

  14. Creation and investigation of powerful EUV sources (λ ≈ 13.5 nm)

    Science.gov (United States)

    Borisov, V. M.; Borisova, G. N.; Vinokhodov, A. Yu.; Ivanov, A. S.; Kiryukhin, Yu. B.; Mishchenko, V. A.; Prokofiev, A. V.; Khristoforov, O. B.

    2010-03-01

    Results are presented from experimental studies of repetitively pulsed EUV (λ = 13.5 ± 0.135 nm) sources based on a laser-initiated discharge in tin vapor between rotating disk electrodes. Radiative characteristics of two sources with different systems of tin supply onto the electrode surface and different types of power supply have been compared. A number of new effects have been revealed at pulse repetition rates as high as ˜4000 Hz. A mean radiation power of 520 W into the 2π solid angle has been achieved in the spectral band 13.5 ± 0.135 nm at a deposited electrical power of 24 kW.

  15. Investigating the Feasibility of Geo-Tagged Photographs as Sources of Land Cover Input Data

    Directory of Open Access Journals (Sweden)

    Vyron Antoniou

    2016-05-01

    Full Text Available Geo-tagged photographs are used increasingly as a source of Volunteered Geographic Information (VGI, which could potentially be used for land use and land cover applications. The purpose of this paper is to analyze the feasibility of using this source of spatial information for three use cases related to land cover: Calibration, validation and verification. We first provide an inventory of the metadata that are collected with geo-tagged photographs and then consider what elements would be essential, desirable, or unnecessary for the aforementioned use cases. Geo-tagged photographs were then extracted from Flickr, Panoramio and Geograph for an area of London, UK, and classified based on their usefulness for land cover mapping including an analysis of the accompanying metadata. Finally, we discuss protocols for geo-tagged photographs for use of VGI in relation to land cover applications.

  16. Beam heat load investigations with a cold vacuum chamber for diagnostics in a synchrotron light source

    Energy Technology Data Exchange (ETDEWEB)

    Voutta, Robert

    2016-04-22

    The beam heat load is a crucial input parameter for the cryogenic design of superconducting insertion devices. To understand the discrepancies between the predicted heat load of an electron beam to a cold bore and the heat load observed in superconducting devices, a cold vacuum chamber for diagnostics has been built. Extensive beam heat load measurements were performed at the Diamond light source. They are analysed systematically and combined with complementary impedance bench measurements.

  17. Investigating microearthquake finite source attributes with IRIS Community Wavefield Demonstration Experiment in Oklahoma

    Science.gov (United States)

    Fan, Wenyuan; McGuire, Jeffrey J.

    2018-05-01

    An earthquake rupture process can be kinematically described by rupture velocity, duration and spatial extent. These key kinematic source parameters provide important constraints on earthquake physics and rupture dynamics. In particular, core questions in earthquake science can be addressed once these properties of small earthquakes are well resolved. However, these parameters of small earthquakes are poorly understood, often limited by available datasets and methodologies. The IRIS Community Wavefield Experiment in Oklahoma deployed ˜350 three component nodal stations within 40 km2 for a month, offering an unprecedented opportunity to test new methodologies for resolving small earthquake finite source properties in high resolution. In this study, we demonstrate the power of the nodal dataset to resolve the variations in the seismic wavefield over the focal sphere due to the finite source attributes of a M2 earthquake within the array. The dense coverage allows us to tightly constrain rupture area using the second moment method even for such a small earthquake. The M2 earthquake was a strike-slip event and unilaterally propagated towards the surface at 90 per cent local S- wave speed (2.93 km s-1). The earthquake lasted ˜0.019 s and ruptured Lc ˜70 m by Wc ˜45 m. With the resolved rupture area, the stress-drop of the earthquake is estimated as 7.3 MPa for Mw 2.3. We demonstrate that the maximum and minimum bounds on rupture area are within a factor of two, much lower than typical stress drop uncertainty, despite a suboptimal station distribution. The rupture properties suggest that there is little difference between the M2 Oklahoma earthquake and typical large earthquakes. The new three component nodal systems have great potential for improving the resolution of studies of earthquake source properties.

  18. Investigating Diesel Engines as an Atmospheric Source of Isocyanic Acid in Urban Areas

    Science.gov (United States)

    Farmer, D.; Jathar, S.; Heppding, C.; Link, M.; Akherati, A.; Kleeman, M.; De Gouw, J. A.; Veres, P. R.; Roberts, J. M.

    2017-12-01

    Isocyanic acid (HNCO), an acidic gas found in tobacco smoke, urban environments and biomass burning-affected regions, has been linked to adverse health outcomes. Gasoline- and diesel-powered engines and biomass burning are known to emit HNCO and hypothesized to emit precursors such as amides that can photochemically react to produce HNCO in the atmosphere. Increasingly, diesel engines in developed countries like the United States are required to use Selective Catalytic Reduction (SCR) systems to reduce tailpipe emissions of oxides of nitrogen. SCR chemistry is known to produce HNCO as an intermediate product, and SCR systems have been implicated as an atmospheric source of HNCO. In this work, we measure HNCO emissions from an SCR system-equipped diesel engine and, in combination with earlier data, use a three-dimensional chemical transport model (CTM) to simulate the ambient concentrations and source/pathway contributions to HNCO in an urban environment. Engine tests were conducted at three different engine loads, using two different fuels and at multiple operating points. HNCO was measured using an acetate chemical ionization mass spectrometer. The diesel engine was found to emit primary HNCO (3-90 mg kg-fuel-1) but we did not find any evidence that the SCR system or other aftertreatment devices (i.e., oxidation catalyst and particle filter) produced or enhanced HNCO emissions. The CTM predictions compared well with the only available observational data sets for HNCO in urban areas but under-predicted the contribution from secondary processes. The comparison implied that diesel-powered engines were the largest source of HNCO in urban areas. The CTM also predicted that daily-averaged concentrations of HNCO reached a maximum of 110 pptv but were an order of magnitude lower than the 1 ppbv level that could be associated with physiological effects in humans. Precursor contributions from other combustion sources (gasoline and biomass burning) and wintertime conditions

  19. Simulation based investigation of source-detector configurations for non-invasive fetal pulse oximetry

    Directory of Open Access Journals (Sweden)

    Böttrich Marcel

    2015-09-01

    Full Text Available Transabdominal fetal pulse oximetry is a method to monitor the oxygen supply of the unborn child non-invasively. Due to the measurement setup, the received signal of the detector is composed of photons coding purely maternal and photons coding mixed fetal-maternal information. To analyze the wellbeing of the fetus, the fetal signal is extracted from the mixed component. In this paper we assess source-detector configurations, such that the mixed fetal-maternal components of the acquired signals are maximized. Monte-Carlo method is used to simulate light propagation and photon distribution in tissue. We use a plane layer and a spherical layer geometry to model the abdomen of a pregnant woman. From the simulations we extracted the fluence at the detector side for several source-detector distances and analyzed the ratio of the mixed fluence component to total fluence. Our simulations showed that the power of the mixed component depends on the source-detector distance as expected. Further we were able to visualize hot spot areas in the spherical layer model where the mixed fluence ratio reaches the highest level. The results are of high importance for sensor design considering signal composition and quality for non-invasive fetal pulse oximetry.

  20. Investigation of particle transport through the measurement of the electron source in the Texas Experimental Tokamak

    International Nuclear Information System (INIS)

    Klepper, C.C.

    1985-01-01

    The spatial distribution of the electron source was measured spectroscopically in the Texas Experimental Tokamak. The method used involves the measurement of the emissivity of the Balmer α and β lines of neutral hydrogen. Modeling of the corresponding atomic transitions provides a relation between the emissivities and the electron source from the ionization of neutrals. Toroidal distributions were obtained by means of a set of relatively calibrated photodiode amplifier-filter packages referred to as plasma light monitors. Such monitors were distributed toroidally, and attached primarily to radial ports. Specially constructed, absolutely calibrated monitors provided absolute calibration. A scanning, rotating mirror system provided in-out brightness profiles. A TV camera system, viewing the limiter through a tangential port, provided a qualitative description of the poloidal asymmetry. Such description was necessary for the inversion of the rotating mirror data. Using electron density profiles obtained by means of far-infrared interferometry, and integrating the electron sources, the global particle confinement time (tau/sub p/) was computed. Parameter scans were performed in ohmically heated plasmas, varying the toroidal field, the plasma current, the electron density, and the plasma position with respect to the center of the poloidal ring limiter. It was found that tau/sub p/ peaks for a critical density that is independent of the other parameters

  1. Bacterial Adhesion & Blocking Bacterial Adhesion

    DEFF Research Database (Denmark)

    Vejborg, Rebecca Munk

    2008-01-01

    , which influence the transition from a planktonic lifestyle to a sessile lifestyle, have been studied. Protein conditioning film formation was found to influence bacterial adhesion and subsequent biofilm formation considerable, and an aqueous extract of fish muscle tissue was shown to significantly...... tract to the microbial flocs in waste water treatment facilities. Microbial biofilms may however also cause a wide range of industrial and medical problems, and have been implicated in a wide range of persistent infectious diseases, including implantassociated microbial infections. Bacterial adhesion...... is the first committing step in biofilm formation, and has therefore been intensely scrutinized. Much however, still remains elusive. Bacterial adhesion is a highly complex process, which is influenced by a variety of factors. In this thesis, a range of physico-chemical, molecular and environmental parameters...

  2. Bacterial meningitis

    NARCIS (Netherlands)

    Heckenberg, Sebastiaan G. B.; Brouwer, Matthijs C.; van de Beek, Diederik

    2014-01-01

    Bacterial meningitis is a neurologic emergency. Vaccination against common pathogens has decreased the burden of disease. Early diagnosis and rapid initiation of empiric antimicrobial and adjunctive therapy are vital. Therapy should be initiated as soon as blood cultures have been obtained,

  3. Bacterial lipases

    NARCIS (Netherlands)

    Jaeger, Karl-Erich; Ransac, Stéphane; Dijkstra, Bauke W.; Colson, Charles; Heuvel, Margreet van; Misset, Onno

    Many different bacterial species produce lipases which hydrolyze esters of glycerol with preferably long-chain fatty acids. They act at the interface generated by a hydrophobic lipid substrate in a hydrophilic aqueous medium. A characteristic property of lipases is called interfacial activation,

  4. Bacterial stress

    Indian Academy of Sciences (India)

    First page Back Continue Last page Graphics. Bacterial stress. Physicochemical and chemical parameters: temperature, pressure, pH, salt concentration, oxygen, irradiation. Nutritional depravation: nutrient starvation, water shortage. Toxic compounds: Antibiotics, heavy metals, toxins, mutagens. Interactions with other cells: ...

  5. Investigation of Seismic Waves from Non-Natural Sources: A Case Study for Building Collapse and Surface Explosion

    Science.gov (United States)

    Houng, S.; Hong, T.

    2013-12-01

    The nature and excitation mechanism of incidents or non-natural events have been widely investigated using seismological techniques. With introduction of dense seismic networks, small-sized non-natural events such as building collapse and chemical explosions are well recorded. Two representative non-natural seismic sources are investigated. A 5-story building in South Korea, Sampoong department store, was collapsed in June 25, 1995, causing casualty of 1445. This accident is known to be the second deadliest non-terror-related building collapse in the world. The event was well recorded by a local station in ~ 9 km away. P and S waves were recorded weak, while monotonic Rayleigh waves were observed well. The origin time is determined using surface-wave arrival time. The magnitude of event is determined to be 1.2, which coincides with a theoretical estimate based on the mass and volume of building. Synthetic waveforms are modeled for various combinations of velocity structures and source time functions, which allow us to constrain the process of building collapse. It appears that the building was collapsed once within a couple of seconds. We also investigate a M2.1 chemical explosion at a fertilizer plant in Texas on April 18, 2013. It was reported that more than one hundred people were dead or injured by the explosion. Seismic waveforms for nearby stations are collected from Incorporated Research Institution of Seismology (IRIS). The event was well recorded at stations in ~500 km away from the source. Strong acoustic signals were observed at stations in a certain great-circle direction. This observation suggests preferential propagation of acoustic waves depending on atmospheric environment. Waveform cross-correlation, spectral analysis and waveform modeling are applied to understand the source physics. We discuss the nature of source and source excitation mechanism.

  6. AN INVESTIGATION OF THE SOURCES OF EARTH-DIRECTED SOLAR WIND DURING CARRINGTON ROTATION 2053

    Energy Technology Data Exchange (ETDEWEB)

    Fazakerley, A. N.; Harra, L. K.; Van Driel-Gesztelyi, L., E-mail: a.fazakerley@ucl.ac.uk [Mullard Space Science Laboratory, University College London (United Kingdom)

    2016-06-01

    In this work we analyze multiple sources of solar wind through a full Carrington Rotation (CR 2053) by analyzing the solar data through spectroscopic observations of the plasma upflow regions and the in situ data of the wind itself. Following earlier authors, we link solar and in situ observations by a combination of ballistic backmapping and potential-field source-surface modeling. We find three sources of fast solar wind that are low-latitude coronal holes. The coronal holes do not produce a steady fast wind, but rather a wind with rapid fluctuations. The coronal spectroscopic data from Hinode ’s Extreme Ultraviolet Imaging Spectrometer show a mixture of upflow and downflow regions highlighting the complexity of the coronal hole, with the upflows being dominant. There is a mix of open and multi-scale closed magnetic fields in this region whose (interchange) reconnections are consistent with the up- and downflows they generate being viewed through an optically thin corona, and with the strahl directions and freeze-in temperatures found in in situ data. At the boundary of slow and fast wind streams there are three short periods of enhanced-velocity solar wind, which we term intermediate based on their in situ characteristics. These are related to active regions that are located beside coronal holes. The active regions have different magnetic configurations, from bipolar through tripolar to quadrupolar, and we discuss the mechanisms to produce this intermediate wind, and the important role that the open field of coronal holes adjacent to closed-field active regions plays in the process.

  7. An Investigation of the Sources of Earth-directed Solar Wind during Carrington Rotation 2053

    Science.gov (United States)

    Fazakerley, A. N.; Harra, L. K.; van Driel-Gesztelyi, L.

    2016-06-01

    In this work we analyze multiple sources of solar wind through a full Carrington Rotation (CR 2053) by analyzing the solar data through spectroscopic observations of the plasma upflow regions and the in situ data of the wind itself. Following earlier authors, we link solar and in situ observations by a combination of ballistic backmapping and potential-field source-surface modeling. We find three sources of fast solar wind that are low-latitude coronal holes. The coronal holes do not produce a steady fast wind, but rather a wind with rapid fluctuations. The coronal spectroscopic data from Hinode’s Extreme Ultraviolet Imaging Spectrometer show a mixture of upflow and downflow regions highlighting the complexity of the coronal hole, with the upflows being dominant. There is a mix of open and multi-scale closed magnetic fields in this region whose (interchange) reconnections are consistent with the up- and downflows they generate being viewed through an optically thin corona, and with the strahl directions and freeze-in temperatures found in in situ data. At the boundary of slow and fast wind streams there are three short periods of enhanced-velocity solar wind, which we term intermediate based on their in situ characteristics. These are related to active regions that are located beside coronal holes. The active regions have different magnetic configurations, from bipolar through tripolar to quadrupolar, and we discuss the mechanisms to produce this intermediate wind, and the important role that the open field of coronal holes adjacent to closed-field active regions plays in the process.

  8. Investigating the Origins of Two Extreme Solar Particle Events: Proton Source Profile and Associated Electromagnetic Emissions

    Energy Technology Data Exchange (ETDEWEB)

    Kocharov, Leon; Usoskin, Ilya [Sodankylä Geophysical Observatory/Oulu Unit, University of Oulu, P.O.B. 3000, Oulu FI-90014 (Finland); Pohjolainen, Silja [Tuorla Observatory, University of Turku, Piikkiö FI-21500 (Finland); Mishev, Alexander [Space Climate Research Unit, University of Oulu, Oulu FI-90014 (Finland); Reiner, Mike J. [The Catholic University of America, Washington, DC, and NASA/Goddard Space Flight Center, Greenbelt, MD (United States); Lee, Jeongwoo [Department of Physics and Astronomy, Seoul National University, Seoul 151-747 (Korea, Republic of); Laitinen, Timo [Jeremiah Horrocks Institute, University of Central Lancashire, Preston PR1 2HE (United Kingdom); Didkovsky, Leonid V. [University of Southern California Space Sciences Center, 835 Bloom Walk, Los Angeles CA 90089 (United States); Pizzo, Victor J. [NOAA Space Weather Prediction Center, Boulder, CO 80305 (United States); Kim, Roksoon; Cho, Kyung-Suk [Korea Astronomy and Space Science Institute, Daejeon 305-348 (Korea, Republic of); Klassen, Andreas [Institut für Experimentelle und Angewandte Physik, Christian-Albrechts-Universität, Kiel D-24118 (Germany); Karlicky, Marian [Astronomical Institute of the Czech Academy of Sciences, Fričova 258, Ondřejov 251 65 (Czech Republic); Gary, Dale E. [Center for Solar-Terrestrial Research, New Jersey Institute of Technology, Newark NJ 07102-1982 (United States); Valtonen, Eino; Vainio, Rami [Space Research Laboratory, University of Turku, Turku FI-20014 (Finland)

    2017-04-20

    We analyze the high-energy particle emission from the Sun in two extreme solar particle events in which protons are accelerated to relativistic energies and can cause a significant signal even in the ground-based particle detectors. Analysis of a relativistic proton event is based on modeling of the particle transport and interaction, from a near-Sun source through the solar wind and the Earth’s magnetosphere and atmosphere to a detector on the ground. This allows us to deduce the time profile of the proton source at the Sun and compare it with observed electromagnetic emissions. The 1998 May 2 event is associated with a flare and a coronal mass ejection (CME), which were well observed by the Nançay Radioheliograph, thus the images of the radio sources are available. For the 2003 November 2 event, the low corona images of the CME liftoff obtained at the Mauna Loa Solar Observatory are available. Those complementary data sets are analyzed jointly with the broadband dynamic radio spectra, EUV images, and other data available for both events. We find a common scenario for both eruptions, including the flare’s dual impulsive phase, the CME-launch-associated decimetric-continuum burst, and the late, low-frequency type III radio bursts at the time of the relativistic proton injection into the interplanetary medium. The analysis supports the idea that the two considered events start with emission of relativistic protons previously accelerated during the flare and CME launch, then trapped in large-scale magnetic loops and later released by the expanding CME.

  9. Investigation of the sources and evolution processes of severe haze pollution in Beijing in January 2013

    Science.gov (United States)

    Sun, Yele; Jiang, Qi; Wang, Zifa; Fu, Pingqing; Li, Jie; Yang, Ting; Yin, Yan

    2014-04-01

    China experienced severe haze pollution in January 2013. Here we have a detailed characterization of the sources and evolution mechanisms of this haze pollution with a focus on four haze episodes that occurred during 10-14 January in Beijing. The main source of data analyzed is from submicron aerosol measurements by an Aerodyne Aerosol Chemical Speciation Monitor. The average PM1 mass concentration during the four haze episodes ranged from 144 to 300 µg m-3, which was more than 10 times higher than that observed during clean periods. All submicron aerosol species showed substantial increases during haze episodes with sulfate being the largest. Secondary inorganic species played enhanced roles in the haze formation as suggested by their elevated contributions during haze episodes. Positive matrix factorization analysis resolved six organic aerosol (OA) factors including three primary OA (POA) factors from traffic, cooking, and coal combustion emissions, respectively, and three secondary OA (SOA) factors. Overall, SOA contributed 41-59% of OA with the rest being POA. Coal combustion OA (CCOA) was the largest primary source, on average accounting for 20-32% of OA, and showed the most significant enhancement during haze episodes. A regional SOA (RSOA) was resolved for the first time which showed a pronounced peak only during the record-breaking haze episode (Ep3) on 12-13 January. The regional contributions estimated based on the steep evolution of air pollutants were found to play dominant roles for the formation of Ep3, on average accounting for 66% of PM1 during the peak of Ep3 with sulfate, CCOA, and RSOA being the largest fractions (> ~ 75%). Our results suggest that stagnant meteorological conditions, coal combustion, secondary production, and regional transport are four main factors driving the formation and evolution of haze pollution in Beijing during wintertime.

  10. Investigating diesel engines as an atmospheric source of isocyanic acid in urban areas

    Directory of Open Access Journals (Sweden)

    S. H. Jathar

    2017-07-01

    Full Text Available Isocyanic acid (HNCO, an acidic gas found in tobacco smoke, urban environments, and biomass-burning-affected regions, has been linked to adverse health outcomes. Gasoline- and diesel-powered engines and biomass burning are known to emit HNCO and hypothesized to emit precursors such as amides that can photochemically react to produce HNCO in the atmosphere. Increasingly, diesel engines in developed countries like the United States are required to use selective catalytic reduction (SCR systems to reduce tailpipe emissions of oxides of nitrogen. SCR chemistry is known to produce HNCO as an intermediate product, and SCR systems have been implicated as an atmospheric source of HNCO. In this work, we measure HNCO emissions from an SCR system-equipped diesel engine and, in combination with earlier data, use a three-dimensional chemical transport model (CTM to simulate the ambient concentrations and source/pathway contributions to HNCO in an urban environment. Engine tests were conducted at three different engine loads, using two different fuels and at multiple operating points. HNCO was measured using an acetate chemical ionization mass spectrometer. The diesel engine was found to emit primary HNCO (3–90 mg kg fuel−1 but we did not find any evidence that the SCR system or other aftertreatment devices (i.e., oxidation catalyst and particle filter produced or enhanced HNCO emissions. The CTM predictions compared well with the only available observational datasets for HNCO in urban areas but underpredicted the contribution from secondary processes. The comparison implied that diesel-powered engines were the largest source of HNCO in urban areas. The CTM also predicted that daily-averaged concentrations of HNCO reached a maximum of ∼ 110 pptv but were an order of magnitude lower than the 1 ppbv level that could be associated with physiological effects in humans. Precursor contributions from other combustion sources (gasoline and biomass

  11. Experimental investigation of thermal limits in parallel plate configuration for the Advanced Neutron Source Reactor

    International Nuclear Information System (INIS)

    Siman-Tov, M.; Felde, D.K.; Kaminaga, M.; Yoder, G.L.

    1993-01-01

    The Advanced Neutron Source Reactor (ANSR) is currently being designed to become the world's highest-flux, steady-state, thermal neutron source for scientific experiments. Highly subcooled, heavy-water coolant flows vertically upward at a very high velocity of 25 m/s through parallel aluminum fuel-plates. The core has average and peak heat fluxes of 5.9 and 12 MW/m 2 , respectively. In this configuration, both flow excursion (FE) and true critical heat flux (CHF), represent potential thermal limitations. The availability of experimental data for both FE and true CHF at the conditions applicable to the ANSR is very limited. A Thermal Hydraulic Test Loop (THTL) facility was designed and built to simulate a full-length coolant subchannel of the core, allowing experimental determination of both thermal limits under the expected ANSR T/H conditions. A series of FE tests with water flowing vertically upward was completed over a nominal heat flux range of 6 to 14 MW/m 2 and a corresponding velocity range of 8 to 21 m/s. Both the exit pressure (1.7 MPa) and inlet temperature (45 degrees C) were maintained constant for these tests, while the loop was operated in a ''stiff''(constant flow) mode. Limited experiments were also conducted at 12 MW/m 2 using a ''soft'' mode (near constant pressure-drop) for actual FE burnout tests and using a ''stiff' mode for true CHF tests, to compare with the original FE experiments

  12. Investigation of gamma-ray fingerprint identifying mechanism for the types of radiation sources

    CERN Document Server

    Liu Su Ping; Gu Dang Chang; Gong-Jian; Hao Fan Hua; Hu Guang Chun

    2002-01-01

    Radiation fingerprints sometimes can be used to label and identify the radiation resources. For instance, in a future nuclear reduction treaty that requires verification of irreversible dismantling of reduced nuclear warheads, the radiation fingerprints of nuclear warheads are expected to play a key role in labelling and identifying the reduced warheads. It would promote the development of nuclear warheads deep-cuts verification technologies if authors start right now some investigations on the issues related to the radiation fingerprints. The author dedicated to the investigation of gamma-ray fingerprint identifying mechanism for the types of radiation resources. The purpose of the identifying mechanism investigation is to find a credible way to tell whether any two gamma-ray spectral fingerprints that are under comparison are radiated from the same resource. The authors created the spectrum pattern comparison (SPC) to study the comparability of the two radiation fingerprints. Guided by the principle of SPC,...

  13. A functional magnetic resonance imaging investigation of short-term source and item memory for negative pictures.

    Science.gov (United States)

    Mitchell, Karen J; Mather, Mara; Johnson, Marcia K; Raye, Carol L; Greene, Erich J

    2006-10-02

    We investigated the hypothesis that arousal recruits attention to item information, thereby disrupting working memory processes that help bind items to context. Using functional magnetic resonance imaging, we compared brain activity when participants remembered negative or neutral picture-location conjunctions (source memory) versus pictures only. Behaviorally, negative trials showed disruption of short-term source, but not picture, memory; long-term picture recognition memory was better for negative than for neutral pictures. Activity in areas involved in working memory and feature integration (precentral gyrus and its intersect with superior temporal gyrus) was attenuated on negative compared with neutral source trials relative to picture-only trials. Visual processing areas (middle occipital and lingual gyri) showed greater activity for negative than for neutral trials, especially on picture-only trials.

  14. Organic matters: investigating the sources, transport, and fate of organic matter in Fanno Creek, Oregon

    Science.gov (United States)

    Sobieszczyk, Steven; Keith, Mackenzie K.; Goldman, Jami H.; Rounds, Stewart A.

    2015-01-01

    The term organic matter refers to the remnants of all living material. This can include fallen leaves, yard waste, animal waste, downed timber, or the remains of any other plant and animal life. Organic matter is abundant both on land and in water. Investigating organic matter is necessary for understanding the fate and transport of carbon (a major constituent of organic matter).

  15. (CH4)-C-14 Measurements in Greenland Ice: Investigating Last Glacial Termination CH4 Sources

    DEFF Research Database (Denmark)

    Petrenko, V. V.; Smith, A. M.; Brook, E. J.

    2009-01-01

    by direct cosmogenic C-14 production in ice. C-14 of CO was measured to better understand this process and correct the sample (CH4)-C-14. Corrected results suggest that wetland sources were likely responsible for the majority of the Younger Dryas-Preboreal CH4 rise.......The cause of a large increase of atmospheric methane concentration during the Younger Dryas-Preboreal abrupt climatic transition (similar to 11,600 years ago) has been the subject of much debate. The carbon-14 (C-14) content of methane ((CH4)-C-14) should distinguish between wetland and clathrate...... contributions to this increase. We present measurements of (CH4)-C-14 in glacial ice, targeting this transition, performed by using ice samples obtained from an ablation site in west Greenland. Measured (CH4)-C-14 values were higher than predicted under any scenario. Sample (CH4)-C-14 appears to be elevated...

  16. Cytogenic investigations of serious overexposures to an industrial gamma radiography source

    International Nuclear Information System (INIS)

    Sevan'kaev, A.V.; Lloyd, D.C.; Edwards, A.A.; Moquet, J.E.; Nugis, V.Yu.; Mikhailova, G.M.; Potetnya, O.I.; Khvostunov, I.K.; Guskova, A.K.; Baranov, A.E.; Nadejina, N.M.

    2002-01-01

    This paper describes the sequence of events, medical aspects and dose estimations for two radiographers and their driver who were seriously exposed to an iridium-192 industrial radiography source that became detached from its wind-out cable. The men came to medical attention about 1 month later by which time all three were severely leucopenic and one had skin burns on both hands. Doses were estimated by (i) physics calculations combined with their accounts of the event, (ii) the levels of depression of their blood neutrophils, (iii) electron spin resonance on tooth enamel and (iv) blood lymphocyte chromosomal analyses by the conventional dicentric and the fluorescence in situ hybridisation methods. Intercomparison of these methods for estimating doses showed a good level of agreement. In brief, the averaged whole body dose for the most seriously exposed man was about 2.5-3.0 Gy and for the others it was 1.0-2.0 Gy. (author)

  17. Investigating the Keratinolytic Activities of the Bacteria Isolated from Different Sources

    Science.gov (United States)

    Chen, S. X.

    2017-12-01

    Background InformationThe development of microbial keratinase has gained increasing attention over the million tons of keratinous wastes generated by the worldwide livestock population. Keratinous wastes not only serve as the niche for pathogenic microorganisms, but also they collapse environmental balance for being the source of air, soil, and water pollutions. Conversely, microbial keratinases can convert the unexploited keratinous wastes into bioaccessible animal feed. However, because of the recalcitrant structure of keratin, the complete keratin degradation is difficult to achieve with one keratinase as opposed to recombinant keratinases. Therefore, this study is designed to isolate the bacteria from different sources and to evaluate their keratinolytic activities using azokeratin substrates. Materials and Methods Soil, horse hooves, penguin stool, and chicken stool are collected from different sites. These samples are serially diluted and then streaked on feather-meal agar plates. Single colonies are screened for the ability to hydrolyze keratin. The isolate of single colonies is cultivated in a whole-feather medium. The sample is then centrifuged, and the supernatant is used for crude enzyme preparation. Azokeratin is synthesized by coupling bristle keratin with diazotized laurent's acid, and it is used as a substrate for assaying keratinase activity by using a spectrophotometer. ConclusionThis study will compare the keratinase activities on bristle azokeratin substrates from three different samples and thus evaluate the bacterium with the best ability to hydrolyze keratin amongst the three. Further steps will be taken to produce a blend of enzymes generated and select the optimum combination that can most efficiently degrade keratin.

  18. INVESTIGATING THE OPTICAL COUNTERPART CANDIDATES OF FOUR INTEGRAL SOURCES LOCALIZED WITH CHANDRA

    International Nuclear Information System (INIS)

    Özbey Arabacı, Mehtap; Kalemci, Emrah; Tomsick, John A.; Bodaghee, Arash; Halpern, Jules; Chaty, Sylvain; Rodriguez, Jerome; Rahoui, Farid

    2012-01-01

    We report on the optical spectroscopic follow-up observations of the candidate counterparts to four INTEGRAL sources: IGR J04069+5042, IGR J06552–1146, IGR J21188+4901, and IGR J22014+6034. The candidate counterparts were determined with Chandra, and the optical observations were performed with 1.5 m RTT-150 telescope (TÜBİTAK National Observatory, Antalya, Turkey) and 2.4 m Hiltner Telescope (MDM Observatory, Kitt Peak, Arizona). Our spectroscopic results show that one of the two candidates of IGR J04069+5042 and the one observed for IGR J06552–1146 could be active late-type stars in RS CVn systems. However, according to the likelihood analysis based on Chandra and INTEGRAL, two optically weaker sources in the INTEGRAL error circle of IGR J06552–1146 have higher probabilities to be the actual counterpart. The candidate counterparts of IGR J21188+4901 are classified as an active M-type star and a late-type star. Among the optical spectra of four candidates of IGR J22014+6034, two show Hα emission lines, one is a late-type star, and the other is an M type. The likelihood analysis favors a candidate with no distinguishing features in the optical spectrum. Two of the candidates classified as M-type dwarfs, are similar to some IGR candidates claimed to be symbiotic stars. However, some of the prominent features of symbiotic systems are missing in our spectra, and their NIR colors are not consistent with those expected for giants. We consider the IR colors of all IGR candidates claimed to be symbiotic systems and find that low-resolution optical spectrum may not be enough for conclusive identification.

  19. [Bacterial vaginosis].

    Science.gov (United States)

    Romero Herrero, Daniel; Andreu Domingo, Antonia

    2016-07-01

    Bacterial vaginosis (BV) is the main cause of vaginal dysbacteriosis in the women during the reproductive age. It is an entity in which many studies have focused for years and which is still open for discussion topics. This is due to the diversity of microorganisms that cause it and therefore, its difficult treatment. Bacterial vaginosis is probably the result of vaginal colonization by complex bacterial communities, many of them non-cultivable and with interdependent metabolism where anaerobic populations most likely play an important role in its pathogenesis. The main symptoms are an increase of vaginal discharge and the unpleasant smell of it. It can lead to serious consequences for women, such as an increased risk of contracting sexually transmitted infections including human immunodeficiency virus and upper genital tract and pregnancy complications. Gram stain is the gold standard for microbiological diagnosis of BV, but can also be diagnosed using the Amsel clinical criteria. It should not be considered a sexually transmitted disease but it is highly related to sex. Recurrence is the main problem of medical treatment. Apart from BV, there are other dysbacteriosis less characterized like aerobic vaginitis of which further studies are coming slowly but are achieving more attention and consensus among specialists. Copyright © 2016 Elsevier España, S.L.U. All rights reserved.

  20. Investigation of gamma-ray fingerprint identifying mechanism for the types of radiation sources

    International Nuclear Information System (INIS)

    Liu Suping; Wu Huailong; Gu Dangchang; Gong Jian; Hao Fanhua; Hu Guangchun

    2002-01-01

    Radiation fingerprints sometimes can be used to label and identify the radiation resources. For instance, in a future nuclear reduction treaty that requires verification of irreversible dismantling of reduced nuclear warheads, the radiation fingerprints of nuclear warheads are expected to play a key role in labelling and identifying the reduced warheads. It would promote the development of nuclear warheads deep-cuts verification technologies if authors start right now some investigations on the issues related to the radiation fingerprints. The author dedicated to the investigation of gamma-ray fingerprint identifying mechanism for the types of radiation resources. The purpose of the identifying mechanism investigation is to find a credible way to tell whether any two gamma-ray spectral fingerprints that are under comparison are radiated from the same resource. The authors created the spectrum pattern comparison (SPC) to study the comparability of the two radiation fingerprints. Guided by the principle of SPC, the authors programmed a software dedicated to identify the types of radiation resources. The efficiency of the software was tested by a series of experiments with some laboratory gamma-ray resources. The experiments were designed to look into the relations between comparability and radioactive statistics, and the relations between comparability and some measurement conditions such as real time, resource activity and background etc. Two main results can be drawn from the investigation: 1) it is quite feasible to use the concept of spectral comparability to answer the question whether any two gamma-ray fingerprints are identity or not; 2) the identifying mechanism can only identify the types of radiation resources, and cannot identify the individuals with the same type and small differences

  1. Cybervetting internet searches for vetting, investigations, and open-source intelligence

    CERN Document Server

    Appel, Edward J

    2014-01-01

    Section I Behavior and TechnologyThe Internet's Potential for Investigators and Intelligence OfficersIntroductionGrowth of Internet UseA Practitioner's PerspectiveThe SearchInternet Posts and the People They ProfileFinding the NeedlesThe Need for SpeedSufficiency of SearchesNotesBehavior OnlineInternet Use GrowthEvolution of Internet UsesPhysical World, Virtual ActivitiesConnections and DisconnectingNotesUse and Abuse: Crime and Mis

  2. INVESTIGATION OF THE PETROLEUM POLLUTION CAUSED BY THE SHIP SOURCE IN ISTANBUL STRAIT

    OpenAIRE

    Salihoglu, Eren

    2018-01-01

    Introduction.The Istanbul Strait is one of theworld's most dangerous natural waterways in terms of sea traffic. Istanbulstraıt, which have great strategic importance, can cause environmentaldisasters because of the sea accidents that the may occur. Petroleum pollutionthat can arise from these vessels can negatively affect the structure of seawater and the marine life.Aim of the study In this study, oil pollution inİstanbul Strait, accidents and investigated scales were researched, the ef...

  3. Experimental investigation of 1 GW repeatable ultra-wide band pulse radiating source

    International Nuclear Information System (INIS)

    Meng Fanbao; Ma Hongge; Zhou Chuanming; Yang Zhoubing; Lu Wei; Ju Bingquan; Yu Huilong

    2001-01-01

    The single cycle pulse of 1.6 GW peak power with 20 Hz repetition-rate was generated. It radiated a peak power of more than 500 MW with a coaxial biconical antenna. The technological problems of the insulation and energy loss during generating and radiating high peak power ultra-wide band (UWB) pulse have been resolved. The experiments show that the material insulation and dispersion in sub-nanosecond pulse should be investigated deeply

  4. Experimental investigation of 1 GW repeatable ultra-wide band pulse radiating source

    Energy Technology Data Exchange (ETDEWEB)

    Fanbao, Meng; Hongge, Ma; Chuanming, Zhou; Zhoubing, Yang; Wei, Lu; Bingquan, Ju; Huilong, Yu [China Academy of Engineering Physics, Chengdu (China)

    2000-11-01

    The single cycle pulse of 1.6 GW peak power with 20 Hz repetition-rate was generated. It radiated a peak power of more than 500 MW with a coaxial biconical antenna. The technological problems of the insulation and energy loss during generating and radiating high peak power ultra-wide band (UWB) pulse have been resolved. The experiments show that the material insulation and dispersion in subnanosecond pulse should be investigated deeply.

  5. Investigation on the development of the cryogenic thermosiphon for the cold neutron source

    International Nuclear Information System (INIS)

    Cheong, S. G.; Rho, C. H.; Kim, S. H.; Chang, G. T.; Cheong, J. H.; In, S. H.; Choi, C. H.; Kim, Y. G.; Park, I. U.; Choi, E. J.

    2005-03-01

    Survey of international and domestic research activities : examination of the CNS-related thermosiphon research materials to investigate the state of the art, summary of the project work scope based on previous research results. Design and fabrication of the experimental mock-up thermosiphon for the HANARO CNS facility : determination of the design parameters for the experimental apparatus, fabrication of the cryogenic thermosiphon with glass dewar for the purpose of direct visual observation of two-phase phenomena, overall check of the whole fabrication process to investigate potential cryogenic problems and seek the solutions, examination of vacuum insulation method for cryogenic thermosiphon. Performance test of the mock-up thermosiphon : temperature and pressure measurements of the working fluid inside thermosiphon during stable operation under various thermal loads of the moderator, cooling capacity control of the condenser to avoid solidification of the working fluid of thermosiphon, investigation of flooding limit to explore the usefulness of a single transfer tube, liquid quantity and void fraction measurements in the moderator under various thermal loads, experimental verification of self-regulation characteristic with and without buffer tank

  6. Investigation on the development of the cryogenic thermosiphon for the cold neutron source

    Energy Technology Data Exchange (ETDEWEB)

    Cheong, S. G.; Rho, C. H.; Kim, S. H.; Chang, G. T.; Cheong, J. H.; In, S. H.; Choi, C. H.; Kim, Y. G.; Park, I. U.; Choi, E. J. [Korea Advanced Institute of Science and Technology, Daejeon (Korea, Republic of)

    2005-03-15

    Survey of international and domestic research activities : examination of the CNS-related thermosiphon research materials to investigate the state of the art, summary of the project work scope based on previous research results. Design and fabrication of the experimental mock-up thermosiphon for the HANARO CNS facility : determination of the design parameters for the experimental apparatus, fabrication of the cryogenic thermosiphon with glass dewar for the purpose of direct visual observation of two-phase phenomena, overall check of the whole fabrication process to investigate potential cryogenic problems and seek the solutions, examination of vacuum insulation method for cryogenic thermosiphon. Performance test of the mock-up thermosiphon : temperature and pressure measurements of the working fluid inside thermosiphon during stable operation under various thermal loads of the moderator, cooling capacity control of the condenser to avoid solidification of the working fluid of thermosiphon, investigation of flooding limit to explore the usefulness of a single transfer tube, liquid quantity and void fraction measurements in the moderator under various thermal loads, experimental verification of self-regulation characteristic with and without buffer tank.

  7. Sources and contamination rate of port sediments: evidences from dimensional, mineralogical, and chemical investigations

    Science.gov (United States)

    Lucchetti, Gabriella; Cutroneo, Laura; Carbone, Cristina; Consani, Sirio; Vagge, Greta; Canepa, Giuseppe; Capello, Marco

    2017-04-01

    Ports are complex environments due to their complicated geometry (quays, channels, and piers), the presence of human activities (vessel traffic, yards, industries, and discharges), and natural factors (stream and torrent inputs, sea action, and currents). Due to the many activities that take place in a port, sediments and waters are often contaminated by different kinds of chemicals, such as hydrocarbons, dioxins, pesticides, nutrients, and metals. The contamination rate of a port basin is site specific and depends on the sources of contamination in the nearby urban system as well as the port system itself, such as city discharges and sewers, river intake, vessel traffic, factories (Taylor and Owens, 2009). Moreover, two important sources and vehicles of contaminants are: a) anthropogenic road deposited sediments derived from the runoff of the port and city area, and natural road deposited sediments derived from rivers and torrents, and b) airborne particulate matter and sediments (Taylor and Owens, 2009). The Port of Genoa is situated at the apex of the Ligurian Sea in the north western Mediterranean Sea and is characterised by the presence of several commercial activities that have contributed, over the years, and still contribute today, to the contaminant accumulation in both the water column and the bottom sediments. This port basin includes the mouth of several streams and the mouth of the Bisagno and the Polcevera Torrents, along the banks of which can be found several small towns, quarries, factories, and the suburbs of the city of Genoa, a ferry terminal, different container terminals, marinas, dry docks, the coal power plant of Genoa, and different wastewater treatment plant discharges. Starting from these considerations, we have examined the marine environment of a port from the point of view of the water mass circulation, hydrological characteristics, distribution of the sediment grain size, mineralogical characteristics, and metal concentrations of the

  8. Nanoindentation of Pseudomonas aeruginosa bacterial biofilm using atomic force microscopy

    International Nuclear Information System (INIS)

    Baniasadi, Mahmoud; Xu, Zhe; Du, Yingjie; Lu, Hongbing; Minary-Jolandan, Majid; Gandee, Leah; Zimmern, Philippe

    2014-01-01

    Bacterial biofilms are a source of many chronic infections. Biofilms and their inherent resistance to antibiotics are attributable to a range of health issues including affecting prosthetic implants, hospital-acquired infections, and wound infection. Mechanical properties of biofilm, in particular, at micro- and nano-scales, are governed by microstructures and porosity of the biofilm, which in turn may contribute to their inherent antibiotic resistance. We utilize atomic force microscopy (AFM)-based nanoindentation and finite element simulation to investigate the nanoscale mechanical properties of Pseudomonas aeruginosa bacterial biofilm. This biofilm was derived from human samples and represents a medically relevant model. (paper)

  9. Investigation on method of estimating the excitation spectrum of vibration source

    International Nuclear Information System (INIS)

    Zhang Kun; Sun Lei; Lin Song

    2010-01-01

    In practical engineer area, it is hard to obtain the excitation spectrum of the auxiliary machines of nuclear reactor through direct measurement. To solve this problem, the general method of estimating the excitation spectrum of vibration source through indirect measurement is proposed. First, the dynamic transfer matrix between the virtual excitation points and the measure points is obtained through experiment. The matrix combined with the response spectrum at the measure points under practical work condition can be used to calculate the excitation spectrum acts on the virtual excitation points. Then a simplified method is proposed which is based on the assumption that the vibration machine can be regarded as rigid body. The method treats the centroid as the excitation point and the dynamic transfer matrix is derived by using the sub structure mobility synthesis method. Thus, the excitation spectrum can be obtained by the inverse of the transfer matrix combined with the response spectrum at the measure points. Based on the above method, a computing example is carried out to estimate the excitation spectrum acts on the centroid of a electrical pump. By comparing the input excitation and the estimated excitation, the reliability of this method is verified. (authors)

  10. An Investigation on the Effects of Ship Sourced Emissions in Izmir Port, Turkey

    Directory of Open Access Journals (Sweden)

    Halil Saraçoğlu

    2013-01-01

    Full Text Available Maritime transportation is a major source of climate change and air pollution. Shipping emissions cause severe impacts on health and environment. These effects of emissions are emerged especially in territorial waters, inland seas, canals, straits, bays, and port regions. In this paper, exhaust gas emissions from ships in Izmir Port, which is one of the main ports in Turkey, are calculated by the ship activity-based methodology. Total emissions from ships in the port is estimated as 1923 ton y−1 for , 1405 ton y−1 for SO2, 82753 ton y−1 for CO2, ton y−1 for HC, and 165 ton y−1 for PM in the year 2007. These emissions are classified regarding operation modes and types of ships. The results are compared with the other studies including amounts of exhaust pollutants generated by ships. According to the findings, it is clear that the ships calling the Izmir Port are important air polluting causes of the Izmir city and its surroundings.

  11. An Investigation on the Effects of Ship Sourced Emissions in Izmir Port, Turkey

    Science.gov (United States)

    Saraçoğlu, Halil; Kılıç, Alper

    2013-01-01

    Maritime transportation is a major source of climate change and air pollution. Shipping emissions cause severe impacts on health and environment. These effects of emissions are emerged especially in territorial waters, inland seas, canals, straits, bays, and port regions. In this paper, exhaust gas emissions from ships in Izmir Port, which is one of the main ports in Turkey, are calculated by the ship activity-based methodology. Total emissions from ships in the port is estimated as 1923 ton y−1 for NOx, 1405 ton y−1 for SO2, 82753 ton y−1 for CO2, ton y−1 for HC, and 165 ton y−1 for PM in the year 2007. These emissions are classified regarding operation modes and types of ships. The results are compared with the other studies including amounts of exhaust pollutants generated by ships. According to the findings, it is clear that the ships calling the Izmir Port are important air polluting causes of the Izmir city and its surroundings. PMID:24198720

  12. INVESTIGATING PARTICLE ACCELERATION IN PROTOSTELLAR JETS: THE TRIPLE RADIO CONTINUUM SOURCE IN SERPENS

    Energy Technology Data Exchange (ETDEWEB)

    Rodríguez-Kamenetzky, Adriana; Valotto, Carlos [Instituto de Astronomía Teórica y Experimental, (IATE-UNC), X5000BGR Córdoba (Argentina); Carrasco-González, Carlos; Rodríguez, Luis F. [Instituto de Radioastronomía y Astrofísica (IRyA-UNAM), 58089 Morelia, México (Mexico); Araudo, Anabella [University of Oxford, Astrophysics, Keble Road, Oxford OX1 3RH (United Kingdom); Torrelles, José M. [Institut de Ciències de l’Espai (CSIC-IEEC) and Institut de Ciències del Cosmos (UB-IEEC), Martí i Franquès 1, E-08028 Barcelona (Spain); Anglada, Guillem [Instituto de Astrofísica de Andalucía, CSIC, Camino Bajo de Huétor 50, E-18008 Granada (Spain); Martí, Josep [Dept. de Física, EPS de Jaén, Universidad de Jaén, Campus Las Lagunillas s/n, A3-402, E-23071 Jaén (Spain)

    2016-02-10

    While most protostellar jets present free–free emission at radio wavelengths, synchrotron emission has also been proposed to be present in a handful of these objects. The presence of nonthermal emission has been inferred by negative spectral indices at centimeter wavelengths. In one case (the HH 80-81 jet arising from a massive protostar), its synchrotron nature was confirmed by the detection of linearly polarized radio emission. One of the main consequences of these results is that synchrotron emission implies the presence of relativistic particles among the nonrelativistic material of these jets. Therefore, an acceleration mechanism should be taking place. The most probable scenario is that particles are accelerated when the jets strongly impact against the dense envelope surrounding the protostar. Here we present an analysis of radio observations obtained with the Very Large Array of the triple radio source in the Serpens star-forming region. This object is known to be a radio jet arising from an intermediate-mass protostar. It is also one of the first protostellar jets where the presence of nonthermal emission was proposed. We analyze the dynamics of the jet and the nature of the emission and discuss these issues in the context of the physical parameters of the jet and the particle acceleration phenomenon.

  13. Investigation of flow asymmetry and instability in the liquid mercury target of the Spallation Neutron Source

    International Nuclear Information System (INIS)

    Pointer, D.; Ruggles, A.; Wendel, M.; Crye, J.

    2000-01-01

    The Spallation Neutron Source (SNS) will utilize a liquid mercury target placed in the path of a high-energy proton beam to produce neutrons for research activities. As the high-energy protons interact with the mercury target, the majority of the beam energy is converted to thermal energy. The liquid mercury must provide sufficient heat transfer to maintain the temperature of the target structure within the thermal limits of the structural materials. Therefore, the behavior of the liquid mercury flow must be characterized in sufficient detail to ensure accurate evaluation of heat transfer in the mercury target. A combination of experimental and computational methods is utilized to characterize the flow in these preliminary analyses. Preliminary studies of the liquid mercury flow in the SNS target indicate that the flow in the exit channel may exhibit multiple recirculation zones, flow asymmetries, and possibly large-scale flow instabilities. While these studies are not conclusive, they serve to focus the efforts of subsequent CFD modeling and experimental programs to better characterize the flow patterns in the SNS mercury target

  14. Thermal-hydraulics investigations for the Liquid Lead-Bismuth Target of the SINQ spallation source

    International Nuclear Information System (INIS)

    Sigg, B.; Dury, T.; Hudina, M.; Smith, B.

    1991-01-01

    The paper contains a discussion of the thermal-hydraulic problems of the target which require detailed analysis by means of a two- or three-dimensional space- and in part also time-dependent fluid dynamics code. There follows a short description of the general-purpose code ASTEC, which is being used for these investigations, and examples of the target modelling, including results. The final part of the paper is devoted to a short discussion of experiments against which this application of the code has to be validated. (author)

  15. Archival Legacy Investigations of Circumstellar Environments (ALICE): Statistical assessment of point source detections

    Science.gov (United States)

    Choquet, Élodie; Pueyo, Laurent; Soummer, Rémi; Perrin, Marshall D.; Hagan, J. Brendan; Gofas-Salas, Elena; Rajan, Abhijith; Aguilar, Jonathan

    2015-09-01

    The ALICE program, for Archival Legacy Investigation of Circumstellar Environment, is currently conducting a virtual survey of about 400 stars, by re-analyzing the HST-NICMOS coronagraphic archive with advanced post-processing techniques. We present here the strategy that we adopted to identify detections and potential candidates for follow-up observations, and we give a preliminary overview of our detections. We present a statistical analysis conducted to evaluate the confidence level on these detection and the completeness of our candidate search.

  16. Multiobjective optimization of cluster-scale urban water systems investigating alternative water sources and level of decentralization

    Science.gov (United States)

    Newman, J. P.; Dandy, G. C.; Maier, H. R.

    2014-10-01

    In many regions, conventional water supplies are unable to meet projected consumer demand. Consequently, interest has arisen in integrated urban water systems, which involve the reclamation or harvesting of alternative, localized water sources. However, this makes the planning and design of water infrastructure more difficult, as multiple objectives need to be considered, water sources need to be selected from a number of alternatives, and end uses of these sources need to be specified. In addition, the scale at which each treatment, collection, and distribution network should operate needs to be investigated. In order to deal with this complexity, a framework for planning and designing water infrastructure taking into account integrated urban water management principles is presented in this paper and applied to a rural greenfield development. Various options for water supply, and the scale at which they operate were investigated in order to determine the life-cycle trade-offs between water savings, cost, and GHG emissions as calculated from models calibrated using Australian data. The decision space includes the choice of water sources, storage tanks, treatment facilities, and pipes for water conveyance. For each water system analyzed, infrastructure components were sized using multiobjective genetic algorithms. The results indicate that local water sources are competitive in terms of cost and GHG emissions, and can reduce demand on the potable system by as much as 54%. Economies of scale in treatment dominated the diseconomies of scale in collection and distribution of water. Therefore, water systems that connect large clusters of households tend to be more cost efficient and have lower GHG emissions. In addition, water systems that recycle wastewater tended to perform better than systems that captured roof-runoff. Through these results, the framework was shown to be effective at identifying near optimal trade-offs between competing objectives, thereby enabling

  17. Investigation on the ultrasonic propagation mechanism and its application on air-source heat pump defrosting

    International Nuclear Information System (INIS)

    Tan, Haihui; Xu, Guanghua; Tao, Tangfei; Zhang, Sicong; Luo, Ailing

    2016-01-01

    Highlights: • Optimal defrosting mode for finned-tube evaporator is S0 mode. • Stress excited by ultrasonic vibration is larger than ice adhesion stress 0.4 MPa. • Frequency matching can enhance the defrosting efficiency effectively. • Ultrasonic vibration can effectively suppressing the frost deposition. • Thermal comfort and heat transfer efficiency enhanced with ultrasonic vibration. - Abstract: Frosting deposited on the outdoor coil of air-source heat pump (ASHP) units deteriorates the operational performance and energy efficiency. Therefore, periodic defrosting is necessary. First, the dispersion curves for the propagation mechanism of an ultrasonic guided wave in the evaporator are determined through numerical calculation. In addition, the shear stress and vibration characteristics under ultrasonic excitation are analysed using finite element method (FEM). Finally, the vibration amplitude and defrosting performance of ultrasonic vibration is analysed. The numerical calculation results indicate that three guided wave modes exist in the evaporator, including both A0 and S0 modes of the Lamb wave and SH0 mode of the SH wave, with the optimal defrosting mode being S0 of the Lamb wave. The FEM results show that the vibrational shapes of S0 mode and longitudinal mode clearly exists in the fin and tube, the torsional and flexural modes also exist in the tube, and the FEM results are consistent with the numerical calculation results. The impedance analysis and laser vibrometer results indicate that the resonance frequency shifting, electro-acoustic converting efficiency and vibration energy decrease is due to increasing external load. The ultrasonic defrosting experimental results indicate that ultrasonic vibration can suppress frost deposit on the fin surface.

  18. Sources, responses and moderators of childbirth fear in Australian women: a qualitative investigation.

    Science.gov (United States)

    Fenwick, J; Toohill, J; Creedy, D K; Smith, J; Gamble, J

    2015-01-01

    around 20% of women suffer childbirth fear causing them significant distress and often leading to requests for caesarean section. In Sweden, fearful pregnant women are offered counselling; however, in Australia, no dedicated service caters for the specific needs of these women. Indeed scant research has been conducted in Australia and little is known about women's concerns and if these align to those reported in the international literature. to describe the sources, responses and moderators of childbirth fear in a group of pregnant women assessed as having high levels of childbirth fear. comparative analysis was used to identify common concepts and generate themes that represented women's perspectives of childbirth fear. Data consisted of 43 tape recorded telephone conversations with highly fearful pregnant women who were participating in a large randomised controlled trial known as BELIEF (Birth Emotions, Looking to Improve Expectant Fear). women's fears were conceptualised into three themes: fear stimuli; fear responses; and fear moderators. Lack of confidence to birth, fear of the unknown, internalising other women's negative stories, perineal tearing and labour pain were common concerns for first time mothers. For multiparous women, not having had personal feelings resolved following their previous birth and negative experiences of last birth influenced current expectations for their upcoming birth. Themes common to both groups were: unmet information and support needs, feelings of loss of control and lack of input in to decision-making. Some women however, chose to avoid birth planning in order to cope during pregnancy. Australian women had similar childbirth concerns to those reported in the international literature. However unique to this study was finding two opposing discourses; one of preoccupation with negative events and the other; avoidance of planning for labour and birth. Provision of woman centred maternity models that minimise obstetric

  19. Localization of epileptogenic zones in Lennox–Gastaut syndrome using frequency domain source imaging of intracranial electroencephalography: a preliminary investigation

    International Nuclear Information System (INIS)

    Cho, Jae-Hyun; Jung, Young-Jin; Kim, Jeong-Youn; Im, Chang-Hwan; Kang, Hoon-Chul; Kim, Heung Dong; Yoon, Dae Sung; Lee, Yong-Ho

    2013-01-01

    Although intracranial electroencephalography (iEEG) has been widely used to localize epileptogenic zones in epilepsy, visual inspection of iEEG recordings does not always result in a favorable surgical outcome, especially in secondary generalized epilepsy such as Lennox–Gastaut syndrome (LGS). Various computational iEEG analysis methods have recently been introduced to confirm the visual inspection results. Of these methods, high gamma oscillation in iEEG has attracted interest because a series of studies have reported a close relationship between epileptogenic zones and cortical areas with high gamma oscillation. Meanwhile, frequency domain source imaging of EEG and MEG oscillations has proven to be a useful auxiliary tool for identifying rough locations of epileptogenic zones. To the best of our knowledge, however, frequency domain source imaging of high gamma iEEG oscillations has not been studied. In this study, we investigated whether the iEEG-based frequency domain source imaging of high gamma oscillation (60–100 Hz) would be a useful supplementary tool for identifying epileptogenic zones in patients with secondary generalized epilepsy. The method was applied to three successfully operated on LGS patients, whose iEEG contained some ictal events with distinct high gamma oscillations before seizure onset. The resultant cortical source distributions were compared with surgical resection areas and with high gamma spectral power distributions on the intracranial sensor plane. While the results of the sensor-level analyses contained many spurious activities, the results of frequency domain source imaging coincided better with the surgical resection areas, suggesting that the frequency domain source imaging of iEEG high gamma oscillations might help enhance the accuracy of pre-surgical evaluations of patients with secondary generalized epilepsy. (paper)

  20. An Investigation of the Radiative Effects and Climate Feedbacks of Sea Ice Sources of Sea Salt Aerosol

    Science.gov (United States)

    Horowitz, H. M.; Alexander, B.; Bitz, C. M.; Jaegle, L.; Burrows, S. M.

    2017-12-01

    In polar regions, sea ice is a major source of sea salt aerosol through lofting of saline frost flowers or blowing saline snow from the sea ice surface. Under continued climate warming, an ice-free Arctic in summer with only first-year, more saline sea ice in winter is likely. Previous work has focused on climate impacts in summer from increasing open ocean sea salt aerosol emissions following complete sea ice loss in the Arctic, with conflicting results suggesting no net radiative effect or a negative climate feedback resulting from a strong first aerosol indirect effect. However, the radiative forcing from changes to the sea ice sources of sea salt aerosol in a future, warmer climate has not previously been explored. Understanding how sea ice loss affects the Arctic climate system requires investigating both open-ocean and sea ice sources of sea-salt aerosol and their potential interactions. Here, we implement a blowing snow source of sea salt aerosol into the Community Earth System Model (CESM) dynamically coupled to the latest version of the Los Alamos sea ice model (CICE5). Snow salinity is a key parameter affecting blowing snow sea salt emissions and previous work has assumed constant regional snow salinity over sea ice. We develop a parameterization for dynamic snow salinity in the sea ice model and examine how its spatial and temporal variability impacts the production of sea salt from blowing snow. We evaluate and constrain the snow salinity parameterization using available observations. Present-day coupled CESM-CICE5 simulations of sea salt aerosol concentrations including sea ice sources are evaluated against in situ and satellite (CALIOP) observations in polar regions. We then quantify the present-day radiative forcing from the addition of blowing snow sea salt aerosol with respect to aerosol-radiation and aerosol-cloud interactions. The relative contributions of sea ice vs. open ocean sources of sea salt aerosol to radiative forcing in polar regions is

  1. Experimental and Numerical Investigation of Thermoacoustic Sources Related to High-Frequency Instabilities

    Directory of Open Access Journals (Sweden)

    Mathieu Zellhuber

    2014-03-01

    Full Text Available Flame dynamics related to high-frequency instabilities in gas turbine combustors are investigated using experimental observations and numerical simulations. Two different combustor types are studied, a premix swirl combustor (experiment and a generic reheat combustor (simulation. In both cases, a very similar dynamic behaviour of the reaction zone is observed, with the appearance of transverse displacement and coherent flame wrinkling. From these observations, a model for the thermoacoustic feedback linked to transverse modes is proposed. The model splits heat release rate fluctuations into distinct contributions that are related to flame displacement and variations of the mass burning rate. The decomposition procedure is applied on the numerical data and successfully verified by comparing a reconstructed Rayleigh index with the directly computed value. It thus allows to quantify the relative importance of various feedback mechanisms for a given setup.

  2. Investigation of novel shape-controlled linearly and circularly polarized attosecond pulse sources

    International Nuclear Information System (INIS)

    Tóth, György; Tibai, Zoltán; Nagy-Csiha, Zsuzsanna; Márton, Zsuzsanna; Almási, Gábor; Hebling, János

    2016-01-01

    In this article, we investigate the temporal shape of one- or few-cycle, 20–180 nm central wavelength attosecond pulses that are produced in a scheme based on coherent undulator radiation. It is demonstrated, that the carrier–envelope phase (CEP) of the radiated electric field can be chosen arbitrarily by shaping the magnetic field of the radiator undulator appropriately. It is shown that the temporal shape and the spectrum of the generated electric field are influenced by the spatial shape and amplitude of the magnetic field of the radiator undulator for different central wavelength pulses, while both are practically independent of the energy of the initial electron bunch. Shape distortions at high K undulator parameters are also discussed.

  3. Investigation of novel shape-controlled linearly and circularly polarized attosecond pulse sources

    Energy Technology Data Exchange (ETDEWEB)

    Tóth, György [MTA-PTE High-Field Terahertz Research Group, 7624 Pécs (Hungary); Tibai, Zoltán; Nagy-Csiha, Zsuzsanna [Institute of Physics, University of Pécs, 7624 Pécs (Hungary); Márton, Zsuzsanna [MTA-PTE High-Field Terahertz Research Group, 7624 Pécs (Hungary); Institute of Physics, University of Pécs, 7624 Pécs (Hungary); Almási, Gábor; Hebling, János [MTA-PTE High-Field Terahertz Research Group, 7624 Pécs (Hungary); Institute of Physics, University of Pécs, 7624 Pécs (Hungary); Szentágothai Research Centre, 7624 Pécs (Hungary)

    2016-02-15

    In this article, we investigate the temporal shape of one- or few-cycle, 20–180 nm central wavelength attosecond pulses that are produced in a scheme based on coherent undulator radiation. It is demonstrated, that the carrier–envelope phase (CEP) of the radiated electric field can be chosen arbitrarily by shaping the magnetic field of the radiator undulator appropriately. It is shown that the temporal shape and the spectrum of the generated electric field are influenced by the spatial shape and amplitude of the magnetic field of the radiator undulator for different central wavelength pulses, while both are practically independent of the energy of the initial electron bunch. Shape distortions at high K undulator parameters are also discussed.

  4. Investigating the Source of Water and/or Hydroxyl on Asteroid (16) Psyche

    Science.gov (United States)

    Takir, D.; Reddy, V.; Sanchez, J. A.; Shepard, M. K.; Emery, J. P.

    2017-12-01

    Asteroid (16) Psyche will be visited by the Psyche mission, which was selected by NASA and will be launched in 2022 as the 14th Discovery mission. Psyche is thought to be one of the most massive exposed metallic core in the asteroid belt. The high radar albedos, thermal inertia, and density of Psyche revealed that this asteroid is composed of almost entirely of Fe-Ni metal. Psyche is also characterized by moderately red spectra and the presence of weak features (attributed to silicates) in the visible and near-infrared (NIR) region (0.3-2.5 µm). Recent NIR observations also showed rotational spectral variations indicating a possible change in the metal/silicate ratio on the surface of this asteroid. Additionally, we observed Psyche in the 3-µm spectral region using the long-wavelength cross-dispersed (LXD: 1.9-4.2 µm) mode of the SpeX spectrograph/imager at the NASA Infrared Telescope Facility (IRTF). Our observations revealed that Psyche exhibits a 3-µm feature, more likely attributed to water- and/or hydroxyl molecules. While the source of water and/or hydroxyl on Psyche remains unclear, we proposed a few possible mechanisms for their formation: (1) the water/hydroxyl-rich materials detected on Psyche might have been delivered to its surface by carbonaceous impactors (like on Vesta), (2) Psyche may not be entirely exposed metallic, instead, its surface has a core-mantle boundary of a differentiated body that was disrupted by impacts (e.g., Pallasite-like), or (3) the water/hydroxyl-rich materials detected on Psyche is produced by Solar wind implantation (like on the Moon). In this talk we will discuss these three possible mechanisms and hypotheses and how they can be tested prior to the launch of the Psyche spacecraft using predictive laboratory measurements and modeling, and during the spacecraft encounter with the asteroid using the mission main instruments that will include the multispectral imagers, the gamma-ray and neutron spectrometer, and the dual

  5. Characterisation of the bacterial community in expressed prostatic secretions from patients with chronic prostatitis/chronic pelvic pain syndrome and infertile men: a preliminary investigation

    Science.gov (United States)

    Hou, Dong-Sheng; Long, Wen-Min; Shen, Jian; Zhao, Li-Ping; Pang, Xiao-Yan; Xu, Chen

    2012-01-01

    The expressed prostatic secretions (EPSs) of men with chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS), infertile men and normal men were subjected to microbiological study. EPSs were collected from the subjects, which included 26 normal men, 11 infertile patients and 51 CP/CPPS patients. DNA was extracted from each specimen, and the V3 regions of the 16S rRNA genes were amplified using universal bacterial primers. The results showed that the EPS 16S rRNA gene-positive rate in the CP/CPPS and infertile patients was much higher than in the normal men, but without any difference among the three patient groups. The denaturing gradient gel electrophoresis (DGGE) method was used to characterize the EPS bacterial community structure of the prostate fluid from patients with CP/CPPS or infertility issues. Principal component analysis (PCA) and partial least squares (PLS) analyses of PCR-DGGE profiles revealed that the EPS bacterial community structure differed among the three groups. Three bands were identified as the key factors responsible for the discrepancy between CP/CPPS patients and infertile patients (Pprostatitis patients (Pprostate and low urethra tract, when considered as a microenvironment, might play an important role in the maintenance of a healthy male reproductive tract. PMID:22635162

  6. Science Self-Efficacy in the Primary Classroom: Using Mixed Methods to Investigate Sources of Self-Efficacy

    Science.gov (United States)

    Webb-Williams, Jane

    2017-04-01

    Self-efficacy has been shown to influence student engagement, effort and performance as well as course selection and future career choice. Extending our knowledge regarding the development of self-efficacy has important implications for educators and for those concerned about the international uptake of science careers. Previous research has identified four sources that may contribute towards self-efficacy: mastery experiences, vicarious experiences, verbal persuasion and physiological/affective states. Very little research has been conducted within the school environment that looks at the formation of these sources and yet early school experiences have been posited to be a key factor in girls' lack of engagement in post compulsory science education. This paper investigates children's self-efficacy beliefs in science and reports on findings from mixed method research conducted with 182 children aged between 10 and 12 years. Classroom data were collected through focus groups, individual interviews and surveys. Findings revealed that although girls and boys held similar levels of academic performance in science, many girls underestimated their capability. The four sources of self-efficacy identified by Bandura (1997) plus self-regulation as an additional source, were evident in the children's descriptions, with boys being more influenced by mastery experience and girls by a combination of vicarious experience and physiological/affective states. Girl's appraisal of information appeared to operate through a heuristic process whereby girls disregarded salient information such as teacher feedback in favour of reliance on social comparison. Contextual factors were identified. Implications for science teachers are discussed.

  7. The optimisation of an intense Z-pinch discharge as a plasma source for absorption spectroscopy investigations

    International Nuclear Information System (INIS)

    Sandolache, Gabriela; Zoita, Vasile; Iova, Iancu; Fleurier, Claude; Hong, Dunpin; Bauchire, Jean Marc

    2002-01-01

    The characterisation of the low voltage circuit breaker arc from the optical and plasma physics points of view represents an element of importance for understanding the operating mechanism and the current interruption process. The development of the broad band optical absorption spectroscopy method seems to be well adapted in order to perform the circuit breaker arc analysis. A pulsed power device based on a Z-pinch type discharge has been developed as a plasma source for absorption spectroscopy investigations. The spatial extension of this radiation source, its brightness, reproducibility are well adapted to characterize the circuit-breaker arc. In addition, a very short emission period compared to the lifetime of the arc discharge provides an excellent time resolution for the absorption spectroscopy method. The first compression phase of the linear pinch produced in argon has been studied from the point of view of its use as a light source. The initial pressure of argon was varied from 0.5 to 2 mbar and the condenser bank energy from 5.1 to 8.7 kJ. The characterization of the emitted radiation, especially the influence of the condenser bank voltage and the argon pressure on the discharge has been studied. Collapse dynamics of the argon compressional pinch and the spectrally resolved continuum emission at the time of maximum compression have been observed. A very satisfactory plasma source optimisation has been achieved that fulfils the conditions required for the absorption spectroscopy method. (authors)

  8. Typing Style and the Use of Different Sources of Information during Typing: An Investigation Using Self-Reports.

    Science.gov (United States)

    Rieger, Martina; Bart, Victoria K E

    2016-01-01

    We investigated to what extent different sources of information are used in typing on a computer keyboard. Using self-reports 10 finger typists and idiosyncratic typists estimated how much attention they pay to different sources of information during copy typing and free typing and how much they use them for error detection. 10 finger typists reported less attention to the keyboard and the fingers and more attention to the template and the screen than idiosyncratic typists. The groups did not differ in attention to touch/kinaesthesis in copy typing and free typing, but 10 finger typists reported more use of touch/kinaesthesis in error detection. This indicates that processing of tactile/kinaesthetic information may occur largely outside conscious control, as long as no errors occur. 10 finger typists reported more use of internal prediction of movement consequences for error detection than idiosyncratic typists, reflecting more precise internal models. Further in copy typing compared to free typing attention to the template is required, thus leaving less attentional capacity for other sources of information. Correlations showed that higher skilled typists, regardless of typing style, rely more on sources of information which are usually associated with 10 finger typing. One limitation of the study is that only self-reports were used. We conclude that typing task, typing proficiency, and typing style influence how attention is distributed during typing.

  9. Investigating the effect of carbon source on rabies virus glycoprotein production in Pichia pastoris by a transcriptomic approach.

    Science.gov (United States)

    Ben Azoun, Safa; Kallel, Héla

    2017-08-01

    Several factors affect protein expression in Pichia pastoris, one among them is the carbon source. In this work, we studied the effect of this factor on the expression level of rabies virus glycoprotein (RABV-G) in two recombinant clones harboring seven copies of the gene of interest. The expression was driven either by the constitutive glyceraldehyde-3-phosphate dehydrogenase (GAP) promoter or the inducible alcohol oxidase1 (AOX1) promoter. Clones were compared in terms of cell physiology and carbon source metabolism. The transcription levels of 16 key genes involved in the central metabolic pathway, the methanol catabolism, and the oxidative stress were investigated in both clones. Cell size, as a parameter reflecting cell physiological changes, was also monitored. Our results showed that when glucose was used as the sole carbon source, large cells were obtained. Transcript levels of the genes of the central metabolic pathway were also upregulated, whereas antioxidative gene transcript levels were low. By contrast, the use of methanol as a carbon source generated small cells and a shift in carbon metabolism toward the dissimilatory pathway by the upregulation of formaldehyde dehydrogenase gene and the downregulation of those of the central metabolic. These observations are in favor of the use of glucose to enhance the expression of RABV-G in P. pastoris. © 2017 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd.

  10. Gene calling and bacterial genome annotation with BG7.

    Science.gov (United States)

    Tobes, Raquel; Pareja-Tobes, Pablo; Manrique, Marina; Pareja-Tobes, Eduardo; Kovach, Evdokim; Alekhin, Alexey; Pareja, Eduardo

    2015-01-01

    New massive sequencing technologies are providing many bacterial genome sequences from diverse taxa but a refined annotation of these genomes is crucial for obtaining scientific findings and new knowledge. Thus, bacterial genome annotation has emerged as a key point to investigate in bacteria. Any efficient tool designed specifically to annotate bacterial genomes sequenced with massively parallel technologies has to consider the specific features of bacterial genomes (absence of introns and scarcity of nonprotein-coding sequence) and of next-generation sequencing (NGS) technologies (presence of errors and not perfectly assembled genomes). These features make it convenient to focus on coding regions and, hence, on protein sequences that are the elements directly related with biological functions. In this chapter we describe how to annotate bacterial genomes with BG7, an open-source tool based on a protein-centered gene calling/annotation paradigm. BG7 is specifically designed for the annotation of bacterial genomes sequenced with NGS. This tool is sequence error tolerant maintaining their capabilities for the annotation of highly fragmented genomes or for annotating mixed sequences coming from several genomes (as those obtained through metagenomics samples). BG7 has been designed with scalability as a requirement, with a computing infrastructure completely based on cloud computing (Amazon Web Services).

  11. The Lag-Luminosity Relation in the GRB Source-Frame: An Investigation with Swift BAT Bursts

    Science.gov (United States)

    Ukwatta, T. N.; Dhuga, K. S.; Stamatikos, M.; Dermer, C. D.; Sakamoto, T.; Sonbas, E.; Parke, W. C.; Maximon, L. C.; Linnemann, J. T.; Bhat, P. N.; hide

    2012-01-01

    Spectral lag, which is defined as the difference in time of arrival of high and low energy photons, is a common feature in Gamma-ray Bursts (GRBs). Previous investigations have shown a correlation between this lag and the isotropic peak luminosity for long duration bursts. However, most of the previous investigations used lags extracted in the observer-frame only. In this work (based on a sample of 43 Swift long GRBs with known redshifts), we present an analysis of the lag-luminosity relation in the GRB source-frame. Our analysis indicates a higher degree of correlation -0.82+/-0.05 (chance probability of approx 5.5 X 10(exp -5) between the spectral lag and the isotropic peak luminosity, L(sub iso), with a best-fit power-law index of -1.2 +/- 0.2, such that L(sub iso) varies as lag(exp -1.2). In addition, there is an anti-correlation between the source-frame spectral lag and the source-frame peak energy of the burst spectrum, E(sub pk)(1 + z).

  12. Bacterial mitosis

    DEFF Research Database (Denmark)

    Møller-Jensen, Jakob; Borch, Jonas; Dam, Mette

    2003-01-01

    Bacterial DNA segregation takes place in an active and ordered fashion. In the case of Escherichia coli plasmid R1, the partitioning system (par) separates paired plasmid copies and moves them to opposite cell poles. Here we address the mechanism by which the three components of the R1 par system...... act together to generate the force required for plasmid movement during segregation. ParR protein binds cooperatively to the centromeric parC DNA region, thereby forming a complex that interacts with the filament-forming actin-like ParM protein in an ATP-dependent manner, suggesting that plasmid...

  13. Investigations of the physical properties of photoemission polarized electron sources for accelerator applications

    International Nuclear Information System (INIS)

    Dunham, B.M.

    1993-01-01

    This experiment measured the polarization and quantum efficiency as a function of wavelength for the chalcopyrite semiconductor Zn (Ge 0.7 Si 0.3 )As 2 . Also, the onset of space charge growth of a 100 keV electron beam passing through the Illinois/CEBAF polarized electron injection system was studied by measuring the beam emittance as a function of current. Finally the thermal properties of GaAs were investigated by measuring the beam emittance as functions of the excitation laser wavelength and the laser spot size. The experiments were performed of Zn(Ge 0.7 Si 0.3 )As 2 was measured to be ∼19%, much lower than the expected 100%. Also, the expected emittance as a function of current was measured and the onset of space charge effects was found to be ∼0.5 mA, much lower than predicted by the electron gun design program EGUN. Finally, the effective transverse thermal energy of the electrons emitted from GaAs at 100 keV as a function of excitation wavelength was measured by a new method for low beam currents. The electron thermal energy for wavelengths between 840 and 633 nm was found to be ∼33 meV, a factor of 3 lower than for a thermionic electron gun. It was found to increase sharply for photon wavelengths less than 633 nm

  14. Experimental investigation of moderately high temperature water source heat pump with non-azeotropic refrigerant mixtures

    Energy Technology Data Exchange (ETDEWEB)

    Zhang, Shengjun; Wang, Huaixin; Guo, Tao [Department of Thermal Energy and Refrigeration Engineering, School of Mechanical Engineering, Tianjin University, Tianjin 300072 (China)

    2010-05-15

    Experimental investigations were carried out on non-azeotropic refrigerant mixtures, named M1A (mass fraction of 20%R152a and 80%R245fa), M1B (mass fraction of 37% R152a and 63%R245fa) and M1C (mass fraction of 50%R152a and 50%R245fa), based on a water-to-water heat pump system in the condensing temperature range of 70-90 C with a cycle temperature lift of 45 C. Performance of R245fa was tested for comparison. Unfair factors in experimental comparative evaluation research with the same apparatus were identified and corrected. Experimental cycle performance of the mixtures were tested and compared with improved experimental assessment methodology. The results show that all of the mixtures deliver higher discharge temperature, higher heating capacity, higher COP and higher {epsilon}{sub h,c} than R245fa. M1B presents the most excellent cycle performance and is recommended as working fluid for moderate/high temperature heat pump. (author)

  15. Kinetic Investigations of SiMn Slags From Different Mn Sources

    Science.gov (United States)

    Kim, Pyunghwa Peace; Tangstad, Merete

    2018-03-01

    The kinetics of MnO and SiO2 reduction were investigated for Silicomanganese (SiMn) slags using a Thermogravimetric analysis (TGA) between 1773 K and 1923 K (1500 °C and 1650 °C) under CO atmospheric pressure. The charge materials were based on Assmang ore and HC FeMn Slag. Rate models for MnO and SiO2 reduction were applied to describe the metal-producing rates, as shown by the following equations: r_{MnO} = k_{MnO} × A × ( {a_{MnO} - {a_{Mn} }/{K_{T }}} ) r_{{{SiO}2 }} = k_{SiO2} × A × ( {a_{{{SiO}2 }} - {a_{Si} }/{K_{T }}} ). The results show that the choice of raw materials in the charge considerably affected the reduction rate of MnO and SiO2. The highest reduction rate was found to be from charges using HC FeMn slag. The difference in the driving forces was insignificant among the SiMn slags, and the similar slag viscosities could not explain the different reduction rates. Instead, the difference is attributed to small amounts of sulfur and the amount of iron in the charge. In addition, the rate models were applicable to describe the reduction of MnO and SiO2 in SiMn slags.

  16. Rarely reported fungal spores and structures: An overlooked source of probative trace evidence in criminal investigations.

    Science.gov (United States)

    Hawksworth, David L; Wiltshire, Patricia E J; Webb, Judith A

    2016-07-01

    The value of pollen and plant spores as trace evidence has long been established, but it is only in the last eight years that fungal spores have been analysed routinely from the same palynological samples. They have greatly enhanced the specificity of links between people, objects, and places. Most fungal species occupy restricted ecological niches and their distributions can be limited both spatially and geographically. Spores may be dispersed over very short distances from the fungal sporophore,(1) and their presence in any palynological assemblage may indicate a restricted area of ground, or the presence of particular plants (even specific dead plant material). Fungal spores can represent primary, secondary, or even tertiary proxy evidence of a location, and can indicate the presence of a plant even though the plant is not obvious at a crime scene. In some cases, spores from fungi which have rarely been reported, and are considered to be rare, have been of particular value in providing intelligence or evidence of contact. Ten examples are given from case work in which rarely reported or unusual fungi have proved to be important in criminal investigations. Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.

  17. Investigation of A = 152 radioactivities with mass-separated sources: Identification of /sup 152/Lu

    International Nuclear Information System (INIS)

    Toth, K.S.; Sousa, D.C.; Nitschke, J.M.; Wilmarth, P.A.

    1987-01-01

    Nuclides with A = 152 were produced in /sup 58/Ni bombardments of /sup 96/Ru and their decay properties were investigated following on-line mass separation. The isotope /sup 152/Lu (T/sub 1/2/ = 0.7 +- 0.1 s) was identified by γ rays in its β-decay daughter, /sup 152/Yb. Based on its decay characteristics, the parent state has a probable spin and parity assignment of (4,5,6 - ). Several new transitions were observed to follow the β decays of /sup 152/Yb and the /sup 152/Tm low-spin isomer; they established previously unknown levels in both /sup 152/Tm and /sup 152/Er. The additional γ rays in /sup 152/Yb decay reduce from 100% to 88% the direct feeding to the one excited state in /sup 152/Tm that had been known earlier. Nevertheless, the corresponding logft value is calculated to be 3.5, indicating that this is an allowed β transition which connects the 0 + parent with a 1 + excited state in /sup 152/Tm. By comparing the β-decay rates of /sup 148/Dy and /sup 152/Ho, and the α- and β-decay rates of /sup 152/Er, an α branch of 90 +- 4 % was deduced for /sup 152/Er

  18. An electron microscopic investigation into the possible source of new muscle fibres in teleost fish.

    Science.gov (United States)

    Stoiber, W; Sänger, A M

    1996-12-01

    This study is based on transmission electron microscopic (TEM) investigations of deep (fast, 'white') teleost fish muscle proliferation in early developmental stages of three European cyprinid species and the rainbow trout. Our fine structural findings provide evidence that early myotomal growth in these animals may utilize different mechanisms that are activated in close succession during early life history. First, initial enlargement of the deep muscle bulk in the embryo seems to be due to hypertrophy of the somite-cell derived stock of muscle fibres. Second, we suggest that deep muscle growth becomes additionally powered by attachment of presumptive myogenic cells that originate from and proliferate within the adjacent mesenchymal tissue lining. Third, mesenchyme-derived muscle cell precursors are thought to enter the myotomes via the myosepta. After migration between the pre-established muscle fibres these cells may function as myosatellite cells, thus at least partly providing the stem cell population for subsequent rapid hyperplastic growth. Finally, there is evidence that presumptive deep muscle satellite cells also proliferate by mitotic division in situ. A similar process of myogenic cell migration and proliferation may foster intermediate fibre differentiation. The model of myogenic cell migration is discussed in view of in vitro and in vivo data on satellite cell migratory power and with respect to temperature-induced and species dependent differences. As for the latter, our results indicate that patterns of muscle differentiation may diverge between a fast growing salmonid species and a moderately growing cyprinid species of similar final size. The model is compatible with the well-established idea that teleost muscle growth may rely on different subclasses of myosatellite cells.

  19. Investigation of a superthermal ultracold neutron source based on a solid deuterium converter for the TRIGA Mainz reactor

    International Nuclear Information System (INIS)

    Lauer, Thorsten

    2010-01-01

    Research in fundamental physics with the free neutron is one of the key tools for testing the Standard Model at low energies. Most prominent goals in this field are the search for a neutron electric dipole moment (EDM) and the measurement of the neutron lifetime. Significant improvements of the experimental performance using ultracold neutrons (UCN) require reduction of both systematic and statistical errors.The development and construction of new UCN sources based on the superthermal concept is therefore an important step for the success of future fundamental physics with ultracold neutrons. Significant enhancement of today available UCN densities strongly correlates with an efficient use of an UCN converter material. The UCN converter here is to be understood as a medium which reduces the velocity of cold neutrons (CN, velocity of about 600 m/s) to the velocity of UCN (velocity of about 6 m/s).Several big research centers around the world are presently planning or constructing new superthermal UCN sources, which are mainly based on the use of either solid deuterium or superfluid helium as UCN converter.Thanks to the idea of Yu.Pokotilovsky, there exists the opportunity to build competitive UCN sources also at small research reactors of the TRIGA type. Of course these smaller facilities don't promise high UCN densities of several 1000 UCN/cm 3 , but they are able to provide densities around 100 UCN/cm 3 for experiments.In the context of this thesis, it was possible to demonstrate succesfully the feasibility of a superthermal UCN source at the tangential beamport C of the research reactor TRIGA Mainz. Based on a prototype for the future UCN source at the Forschungs-Neutronenquelle Heinz Maier-Leibnitz (FRMII) in Munich, which was planned and built in collaboration with the Technical University of Munich, further investigations and improvements were done and are presented in this thesis. In parallel, a second UCN source for the radial beamport D was designed and

  20. Investigation of a superthermal ultracold neutron source based on a solid deuterium converter for the TRIGA Mainz reactor

    Energy Technology Data Exchange (ETDEWEB)

    Lauer, Thorsten

    2010-12-22

    Research in fundamental physics with the free neutron is one of the key tools for testing the Standard Model at low energies. Most prominent goals in this field are the search for a neutron electric dipole moment (EDM) and the measurement of the neutron lifetime. Significant improvements of the experimental performance using ultracold neutrons (UCN) require reduction of both systematic and statistical errors.The development and construction of new UCN sources based on the superthermal concept is therefore an important step for the success of future fundamental physics with ultracold neutrons. Significant enhancement of today available UCN densities strongly correlates with an efficient use of an UCN converter material. The UCN converter here is to be understood as a medium which reduces the velocity of cold neutrons (CN, velocity of about 600 m/s) to the velocity of UCN (velocity of about 6 m/s).Several big research centers around the world are presently planning or constructing new superthermal UCN sources, which are mainly based on the use of either solid deuterium or superfluid helium as UCN converter.Thanks to the idea of Yu.Pokotilovsky, there exists the opportunity to build competitive UCN sources also at small research reactors of the TRIGA type. Of course these smaller facilities don't promise high UCN densities of several 1000 UCN/cm{sup 3}, but they are able to provide densities around 100 UCN/cm{sup 3} for experiments.In the context of this thesis, it was possible to demonstrate succesfully the feasibility of a superthermal UCN source at the tangential beamport C of the research reactor TRIGA Mainz. Based on a prototype for the future UCN source at the Forschungs-Neutronenquelle Heinz Maier-Leibnitz (FRMII) in Munich, which was planned and built in collaboration with the Technical University of Munich, further investigations and improvements were done and are presented in this thesis. In parallel, a second UCN source for the radial beamport D was

  1. Investigation of bacterial communities within the digestive organs of the hydrothermal vent shrimp Rimicaris exoculata provide insights into holobiont geographic clustering.

    Directory of Open Access Journals (Sweden)

    Dominique A Cowart

    Full Text Available Prokaryotic communities forming symbiotic relationships with the vent shrimp, Rimicaris exoculata, are well studied components of hydrothermal ecosystems at the Mid-Atlantic Ridge (MAR. Despite the tight link between host and symbiont, the observed lack of spatial genetic structure seen in R. exoculata contrasts with the geographic differentiation detected in specific bacterial ectosymbionts. The geographic clustering of bacterial lineages within a seemingly panmictic host suggests either the presence of finer scale restriction to gene flow not yet detected in the host, horizontal transmission (environmental selection of its endosymbionts as a consequence of unique vent geochemistry, or vertically transmitted endosymbionts that exhibit genetic differentiation. To identify which hypothesis best fits, we tested whether bacterial assemblages exhibit differentiation across sites or host populations by performing a 16S rRNA metabarcoding survey on R. exoculata digestive prokaryote samples (n = 31 taken from three geochemically distinct vents across MAR: Rainbow, Trans-Atlantic Geotraverse (TAG and Logatchev. Analysis of communities across two organs (digestive tract, stomach, three molt colors (white, red, black and three life stages (eggs, juveniles, adults also provided insights into symbiont transmission mode. Examining both whole communities and operational taxonomic units (OTUs confirmed the presence of three main epibionts: Epsilonproteobacteria, Mollicutes and Deferribacteres. With these findings, we identified a clear pattern of geographic segregation by vent in OTUs assigned to Epsilonproteobacteria. Additionally, we detected evidence for differentiation among all communities associated to vents and life stages. Overall, results suggest a combination of environmental selection and vertical inheritance of some of the symbiotic lineages.

  2. Characterisation of the bacterial community in expressed prostatic secretions from patients with chronic prostatitis/chronic pelvic pain syndrome and infertile men: a preliminary investigation

    Institute of Scientific and Technical Information of China (English)

    Dong-Sheng Hou; Wen-Min Long; Jian Shen; Li-Ping Zhao; Xiao-Yan Pang; Chen XU

    2012-01-01

    The expressed prostatic secretions (EPSs) of men with chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS),infertile men and normal men were subjected to microbiological study.EPSs were collected from the subjects,which included 26 normal men,11 infertile patients and 51 CP/CPPS patients.DNA was extracted from each specimen,and the V3 regions of the 16S rRNA genes were amplified using universal bacterial primers.The results showed that the EPS 16S rRNA gene-positive rate in the CP/CPPS and infertile patients was much higher than in the normal men,but without any difference among the three patient groups.The denaturing gradient gel electrophoresis (DGGE) method was used to characterize the EPS bacterial community structure of the prostate fluid from patients with CP/CPPS or infertility issues.Principal component analysis (PCA) and partial least squares (PLS) analyses of PCR-DGGE profiles revealed that the EPS bacterial community structure differed among the three groups.Three bands were identified as the key factors responsible for the discrepancy between CP/CPPS patients and infertile patients (P<0.05).Two bands were identified as priority factors in the discrepancy of category ⅢA and category ⅢB prostatitis patients (P<0.05).According to this research,the ecological balance of the prostate and low urethra tract,when considered as a microenvironment,might play an important role in the maintenance of a healthy male reproductive tract.

  3. Experimental investigation and feasibility analysis on a capillary radiant heating system based on solar and air source heat pump dual heat source

    International Nuclear Information System (INIS)

    Zhao, M.; Gu, Z.L.; Kang, W.B.; Liu, X.; Zhang, L.Y.; Jin, L.W.; Zhang, Q.L.

    2017-01-01

    Graphical abstract: (a) Vertical temperature gradient in Case 3, (b) PMV and PPD of the test room in Case 3, (c) operating time of SPCTS and ASHP systems in Case 3 and (d) the proportion of SPCTS operating time. - Highlights: • A capillary heating system based on solar and air source heat pump was developed. • Influence of supply water temperature on solar energy saving rate was investigated. • Heating performance and thermal comfort of capillary heating system were analyzed. • Low temperature heating with capillary is suitable for solar heating system. - Abstract: Due to sustainable development, solar energy has drawn much attention and been widely applied in buildings. However, the application of solar energy is limited because of its instability, intermittency and low energy density in winter. In order to use low density and instable solar energy source for heating and improve the utilization efficiency of solar energy, a solar phase change thermal storage (SPCTS) heating system using a radiant-capillary-terminal (RCT) to effectively match the low temperature hot water, a phase change thermal storage (PCTS) to store and continuously utilize the solar energy, and an air source heat pump (ASHP) as an alternate energy, was proposed and set up in this research. Series of experiments were conducted to obtain the relation between the solar radiation utilization rate and the heating supply temperatures, and to evaluate the performance of the RCT module and the indoor thermal environment of the system for its practical application in a residential building in the north-western City of Xi’an, China. The results show that energy saving of the solar heating system can be significantly improved by reducing the supplied water temperature, and the supplied water temperature of the RCT would be no more than 35 °C. The capillary radiation heating can adopt a lower water temperature and create a good thermal comfort environment as well. These results may lead to the

  4. Heavy metal contamination of surface soil in electronic waste dismantling area: site investigation and source-apportionment analysis.

    Science.gov (United States)

    Jinhui Li; Huabo Duan; Pixing Shi

    2011-07-01

    The dismantling and disposal of electronic waste (e-waste) in developing countries is causing increasing concern because of its impacts on the environment and risks to human health. Heavy-metal concentrations in the surface soils of Guiyu (Guangdong Province, China) were monitored to determine the status of heavy-metal contamination on e-waste dismantling area with a more than 20 years history. Two metalloids and nine metals were selected for investigation. This paper also attempts to compare the data among a variety of e-waste dismantling areas, after reviewing a number of heavy-metal contamination-related studies in such areas in China over the past decade. In addition, source apportionment of heavy metal in the surface soil of these areas has been analysed. Both the MSW open-burning sites probably contained invaluable e-waste and abandoned sites formerly involved in informal recycling activities are the new sources of soil-based environmental pollution in Guiyu. Although printed circuit board waste is thought to be the main source of heavy-metal emissions during e-waste processing, requirement is necessary to soundly manage the plastic separated from e-waste, which mostly contains heavy metals and other toxic substances.

  5. Bacterial Ecology

    DEFF Research Database (Denmark)

    Fenchel, Tom

    2011-01-01

    compounds these must first be undergo extracellular hydrolysis. Bacteria have a great diversity with respect to types of metabolism that far exceeds the metabolic repertoire of eukaryotic organisms. Bacteria play a fundamental role in the biosphere and certain key processes such as, for example......, the production and oxidation of methane, nitrate reduction and fixation of atmospheric nitrogen are exclusively carried out by different groups of bacteria. Some bacterial species – ‘extremophiles’ – thrive in extreme environments in which no eukaryotic organisms can survive with respect to temperature, salinity...... biogeochemical processes are carried exclusively by bacteria. * Bacteria play an important role in all types of habitats including some that cannot support eukaryotic life....

  6. Bacterial Actins.

    Science.gov (United States)

    Izoré, Thierry; van den Ent, Fusinita

    2017-01-01

    A diverse set of protein polymers, structurally related to actin filaments contributes to the organization of bacterial cells as cytomotive or cytoskeletal filaments. This chapter describes actin homologs encoded by bacterial chromosomes. MamK filaments, unique to magnetotactic bacteria, help establishing magnetic biological compasses by interacting with magnetosomes. Magnetosomes are intracellular membrane invaginations containing biomineralized crystals of iron oxide that are positioned by MamK along the long-axis of the cell. FtsA is widespread across bacteria and it is one of the earliest components of the divisome to arrive at midcell, where it anchors the cell division machinery to the membrane. FtsA binds directly to FtsZ filaments and to the membrane through its C-terminus. FtsA shows altered domain architecture when compared to the canonical actin fold. FtsA's subdomain 1C replaces subdomain 1B of other members of the actin family and is located on the opposite side of the molecule. Nevertheless, when FtsA assembles into protofilaments, the protofilament structure is preserved, as subdomain 1C replaces subdomain IB of the following subunit in a canonical actin filament. MreB has an essential role in shape-maintenance of most rod-shaped bacteria. Unusually, MreB filaments assemble from two protofilaments in a flat and antiparallel arrangement. This non-polar architecture implies that both MreB filament ends are structurally identical. MreB filaments bind directly to membranes where they interact with both cytosolic and membrane proteins, thereby forming a key component of the elongasome. MreB filaments in cells are short and dynamic, moving around the long axis of rod-shaped cells, sensing curvature of the membrane and being implicated in peptidoglycan synthesis.

  7. Manganese oxidation and bacterial diversity on different filter media coatings during the start-up of drinking water biofilters

    DEFF Research Database (Denmark)

    Breda, I. L.; Ramsay, L.; Roslev, P.

    2017-01-01

    Manganese removal is a typical concern in drinking water production. Biofiltration may be used when treating groundwater sources but the onset of manganese removal in virgin biofilters can vary considerably. The aim of this study was to investigate the effect of different filter media on manganese...... by manganese oxide, while 48, 57 and 72 days were required by virgin quartz, calcium carbonate and polystyrene, respectively. The bacterial community was investigated using DAPI staining, quantitative polymerase chain reaction (qPCR), 16S rRNA gene pyrosequencing, and bacterial enrichments. Bacterial abundance...

  8. An Investigation of the Correlation among Sources of Stress, Perceived Stress, and Coping styles in High School Students

    Directory of Open Access Journals (Sweden)

    Farhadh Asghari

    2016-07-01

    Full Text Available Background and Objectives: Coping with stress is an important issue, especially in regard to source of stress and perceived stress in adolescence period. In this research, the correlation among stress sources, perceived stress, and coping styles was investigated in high school students. Methods: The present research was a descriptive correlational study. The research sample consisted of 575 high school students from the families of personnel of Bushehr University of Medical Sciences in education year 2014-15 who were selected by multistage cluster sampling method. Stress source scale, perceived stress scale and Tehran coping styles scale, were used for data collection. The data were analyzed using statistical methods, including Pearson correlation coefficient and multivariate regression analysis. Results: In this study, 50% of students had perceived stress higher than cut-off point. Stress sources of students were related to school and maturity. There was a positive correlation between problem-oriented coping style and perceived stress, and there was a negative and inverse correlation between problem-oriented coping style and stress related to school and maturity. There was a positive correlation between positive emotion-oriented coping style and perceived stress, but there was a negative and inverse correlation between problem-oriented coping style and stress related to school and maturity. There was no significant correlation between emotion-oriented coping style and stress related to school, maturity, family, and peers. There was a positive correlation between negative emotion-oriented coping style and stress related to school, maturity, and peers. Conclusion: According to the findings of this study, the level of correlation was not significant and no significant relationship could be found between the variables with this level of correlation. Therefore, it is suggested that more extensive researches be conducted on the relationship between

  9. Investigation of chemical modifiers for phosphorus in a graphite furnace using high-resolution continuum source atomic absorption spectrometry

    International Nuclear Information System (INIS)

    Lepri, Fabio G.; Dessuy, Morgana B.; Vale, Maria Goreti R.; Borges, Daniel L.G.; Welz, Bernhard; Heitmann, Uwe

    2006-01-01

    Phosphorus is not one of the elements that are typically determined by atomic absorption spectrometry, but this technique nevertheless offers several advantages that make it attractive, such as the relatively great freedom from interferences. As the main resonance lines for phosphorus are in the vacuum-ultraviolet, inaccessible by conventional atomic absorption spectrometry equipment, L'vov and Khartsyzov proposed to use the non-resonance doublet at 213.5 / 213.6 nm. Later it turned out that with conventional equipment it is necessary to use a chemical modifier in order to get reasonable sensitivity, and lanthanum was the first one suggested for that purpose. In the following years more than 30 modifiers have been proposed for the determination of this element, and there is no consensus about the best one. In this work high-resolution continuum source atomic absorption spectrometry has been used to investigate the determination of phosphorus without a modifier and with the addition of selected modifiers of very different nature, including the originally recommended lanthanum modifier, several palladium-based modifiers and sodium fluoride. As high-resolution continuum source atomic absorption spectrometry is revealing the spectral environment of the analytical line at high resolution, it became obvious that without the addition of a modifier essentially no atomic phosphorus is formed, even at 2700 deg. C . The absorption measured with line source atomic absorption spectrometry in this case is due to the PO molecule, the spectrum of which is overlapping with the atomic line. Palladium, with or without the addition of calcium or ascorbic acid, was found to be the only modifier to produce almost exclusively atomic phosphorus. Lanthanum and particularly sodium fluoride produced a mixture of P and PO, depending on the atomization temperature. This fact can explain at least some of the discrepancies found in the literature and some of the phenomena observed in the

  10. Bacterial growth kinetics

    International Nuclear Information System (INIS)

    Boonkitticharoen, V.; Ehrhardt, J.C.; Kirchner, P.T.

    1989-01-01

    Quantitative measurement of bacterial growth may be made using a radioassay technique. This method measures, by scintillation counting, the 14 CO 2 derived from the bacterial metabolism of a 14 C-labeled substrate. Mathematical growth models may serve as reliable tools for estimation of the generation rate constant (or slope of the growth curve) and provide a basis for evaluating assay performance. Two models, i.e., exponential and logistic, are proposed. Both models yielded an accurate fit to the data from radioactive measurement of bacterial growth. The exponential model yielded high precision values of the generation rate constant, with an average relative standard deviation of 1.2%. Under most conditions the assay demonstrated no changes in the slopes of growth curves when the number of bacteria per inoculation was changed. However, the radiometric assay by scintillation method had a growth-inhibiting effect on a few strains of bacteria. The source of this problem was thought to be hypersensitivity to trace amounts of toluene remaining on the detector

  11. Diversity of Bacterial Communities of Fitness Center Surfaces in a U.S. Metropolitan Area

    Directory of Open Access Journals (Sweden)

    Nabanita Mukherjee

    2014-12-01

    Full Text Available Public fitness centers and exercise facilities have been implicated as possible sources for transmitting community-acquired bacterial infections. However, the overall diversity of the bacterial community residing on the surfaces in these indoor environments is still unknown. In this study, we investigated the overall bacterial ecology of selected fitness centers in a metropolitan area (Memphis, TN, USA utilizing culture-independent pyrosequencing of the 16S rRNA genes. Samples were collected from the skin-contact surfaces (e.g., exercise instruments, floor mats, handrails, etc. within fitness centers. Taxonomical composition revealed the abundance of Firmicutes phyla, followed by Proteobacter and Actinobacteria, with a total of 17 bacterial families and 25 bacterial genera. Most of these bacterial genera are of human and environmental origin (including, air, dust, soil, and water. Additionally, we found the presence of some pathogenic or potential pathogenic bacterial genera including Salmonella, Staphylococcus, Klebsiella, and Micrococcus. Staphylococcus was found to be the most prevalent genus. Presence of viable forms of these pathogens elevates risk of exposure of any susceptible individuals. Several factors (including personal hygiene, surface cleaning and disinfection schedules of the facilities may be the reasons for the rich bacterial diversity found in this study. The current finding underscores the need to increase public awareness on the importance of personal hygiene and sanitation for public gym users.

  12. Microbial ecology of bacterially mediated PCB biodegradation

    International Nuclear Information System (INIS)

    Pettigrew, C.A. Jr.

    1989-01-01

    The roles of plasmid mediated and consortia mediated polychlorinated biphenyl (PCB) biodegradation by bacterial populations isolated from PCB contaminated freshwater sediments were investigated. PCB degrading bacteria were isolated by DNA:DNA colony hybridization, batch enrichments, and chemostat enrichment. Analysis of substrate removal and metabolite production were done using chlorinated biphenyl spray plates, reverse phase high pressure liquid chromatography, Cl - detection, and 14 C-labeled substrate mineralization methods. A bacterial consortium, designated LPS10, involved in a concerted metabolic attack on chlorinated biphenyls, was shown to mineralize 4-chlorobiphenyl (4CB) and 4,4'-dichlorobiphenyl (4,4' CB). The LPS10 consortium was isolated by both batch and chemostat enrichment using 4CB and biphenyl (BP) as sole carbon source and was found to have tree bacterial isolates that predominated; these included: Pseudomonas, testosteroni LPS10A which mediated the breakdown of 4CB and 4,4' CB to the putative meta-cleavage product and subsequently to 4-chlorobenzoic acid (4CBA), an isolate tentatively identified as an Arthrobacter sp. LPS10B which mediated 4CBA degradation, and Pseudomonas putida by A LPS10C whose role in the consortium has not been determined

  13. An investigation of mercury sources in the Puyango-Tumbes River: Using stable Hg isotopes to characterize transboundary Hg pollution.

    Science.gov (United States)

    Schudel, Gary; Miserendino, Rebecca Adler; Veiga, Marcello M; Velasquez-López, P Colon; Lees, Peter S J; Winland-Gaetz, Sean; Davée Guimarães, Jean Remy; Bergquist, Bridget A

    2018-07-01

    Mercury (Hg) concentrations and stable isotopes along with other trace metals were examined in environmental samples from Ecuador and Peru's shared Puyango-Tumbes River in order to determine the extent to which artisanal- and small-scale gold mining (ASGM) in Portovelo-Zaruma, Ecuador contributes to Hg pollution in the downstream aquatic ecosystem. Prior studies investigated the relationship between ASGM activities and downstream Hg pollution relying primarily on Hg concentration data. In this study, Hg isotopes revealed an isotopically heavy Hg signature with negligible mass independent fractionation (MIF) in downstream sediments, which was consistent with the signature observed in the ASGM source endmember. This signature was traced as far as ∼120 km downstream of Portovelo-Zaruma, demonstrating that Hg stable isotopes can be used as a tool to fingerprint and trace sources of Hg over vast distances in freshwater environments. The success of Hg isotopes as a source tracer in fresh waters is largely due to the particle-reactive nature of Hg. Furthermore, the magnitude and extent of downstream Hg, lead, copper and zinc contamination coupled with the Hg isotopes suggest that it is unlikely that the smaller artisanal-scale activities, which do not use cyanidation, are responsible for the pollution. More likely it is the scale of ores processed and the cyanide leaching, which can release other metals and enhance Hg transport, used during small-scale gold mining that is responsible. Thus, although artisanal- and small-scale gold mining occur in tandem in Portovelo-Zaruma, a distinction should be made between these two activities. Copyright © 2018 Elsevier Ltd. All rights reserved.

  14. CONTROLLING INFLUENCE OF MAGNETIC FIELD ON SOLAR WIND OUTFLOW: AN INVESTIGATION USING CURRENT SHEET SOURCE SURFACE MODEL

    Energy Technology Data Exchange (ETDEWEB)

    Poduval, B., E-mail: bpoduval@spacescience.org [Space Science Institute, Boulder, CO 80303 (United States)

    2016-08-10

    This Letter presents the results of an investigation into the controlling influence of large-scale magnetic field of the Sun in determining the solar wind outflow using two magnetostatic coronal models: current sheet source surface (CSSS) and potential field source surface. For this, we made use of the Wang and Sheeley inverse correlation between magnetic flux expansion rate (FTE) and observed solar wind speed (SWS) at 1 au. During the period of study, extended over solar cycle 23 and beginning of solar cycle 24, we found that the coefficients of the fitted quadratic equation representing the FTE–SWS inverse relation exhibited significant temporal variation, implying the changing pattern of the influence of FTE on SWS over time. A particularly noteworthy feature is an anomaly in the behavior of the fitted coefficients during the extended minimum, 2008–2010 (CRs 2073–2092), which is considered due to the particularly complex nature of the solar magnetic field during this period. However, this variation was significant only for the CSSS model, though not a systematic dependence on the phase of the solar cycle. Further, we noticed that the CSSS model demonstrated better solar wind prediction during the period of study, which we attribute to the treatment of volume and sheet currents throughout the corona and the more accurate tracing of footpoint locations resulting from the geometry of the model.

  15. Cryptococcus neoformans in the koala (Phascolarctos cinereus): colonization by C n. var. gattii and investigation of environmental sources.

    Science.gov (United States)

    Krockenberger, M B; Canfield, P J; Malik, R

    2002-06-01

    This study is the one in a series looking at the relationship among Cryptococcus neoformans var. gattii, koalas and the environment. The koala was used as a natural biological sampler in an attempt to understand the dynamics of C. neoformans var. gattii in Australian environments. Evidence of asymptomatic nasal and skin colonization for extended periods by large numbers of C. n. var. gattii was obtained and geographical factors assessed. The key finding was the ability of koalas to amplify numbers of C. n. var. gattii in certain environments. Koalas were not found to be obligatory for the survival of the organism in all environments. Geographical factors alone could not explain differing rates of nasal and skin colonization in koalas in different environments. A strong association between healthy koalas and C. n. var. gattii was confirmed and C n. var. gattii was isolated from novel sources, including the turpentine gum tree (Syncarpia glomulifera), tallowwood (Eucalyptus microcorys) and flooded gum (E. grandis). It seems likely that as yet undiscovered environmental sources of C. n. var. gattii exist in eastern Australia. Further investigation of host, environmental and organism factors integral to the hostpathogen relationship will assist an understanding of the progression from colonization to tissue invasion and cryptococcosis in all species.

  16. Investigation of Efficiency and Thermal Performance of The Y-source Converters for a Wide Voltage Range

    Directory of Open Access Journals (Sweden)

    Brwene Salah Gadalla

    2015-12-01

    Full Text Available The Y-source topology has a unique advantage of having high voltages gain with small shoot through duty cycles. Furthermore, having the advantage of high modulation index which increase the power density and improve the performance of the converter. In this paper, a collective thermal and efficiency investigation has been performed in order to improve the reliability of the converter. Evaluation of relevant losses as ( switching, conduction, capacitor ESR, core and winding losses , and evaluation of the junction temperature of the devices under 25C ambient temperature. The analysis is done for different voltage gain factors (2, 3, and 4, and different winding factor (4, and 5 using PLECS toolbox. The results shows that the higher the voltage gain and winding factor, the higher power losses and rising in the junction temperature of the device.

  17. An experimental investigation of the shear-layer and acoustic sources produced by a leading edge slat

    Science.gov (United States)

    Wilkins, Stephen; Richard, Patrick; Hall, Joseph; Turbulence; Flow Noise Laboratory Team

    2013-11-01

    Leading edge slats are a common addition to airfoils as part of a high lift configuration employed during take-off and landing; the unsteady flow caused by these slats is a major contributor to the overal airframe noise. As the next generation of aircraft seeks to reduce these noise concerns, a better understanding of the sources of aeroacoustic noise generation is sought. Particle Image Velocimetry (PIV) and simultaneous multipoint measurements of the unsteady surface pressure are used herein to investigate the unsteady flow around a leading edge slat coupled with an airfoil for several different configurations and a range of Reynolds numbers (Re = 156 , 000 to Re = 1 . 2 million based on the wing chord). Shear-layer development off the slat cusp and the related unsteady vortex structures are examined in detail to better establish and understand the mechanisms responsible for the generation of aeroacoustic slat noise. The authors are grateful for the support provided by GARDN.

  18. Bacterial polyhydroxyalkanoates: Still fabulous?

    Science.gov (United States)

    Możejko-Ciesielska, Justyna; Kiewisz, Robert

    2016-11-01

    Bacterial polyhydroxyalkanoates (PHA) are polyesters accumulated as carbon and energy storage materials under limited growth conditions in the presence of excess carbon sources. They have been developed as biomaterials with unique properties for the past many years being considered as a potential substitute for conventional non-degradable plastics. Due to the increasing concern towards global climate change, depleting petroleum resource and problems with an utilization of a growing number of synthetic plastics, PHAs have gained much more attention from industry and research. These environmentally friendly microbial polymers have great potential in biomedical, agricultural, and industrial applications. However, their production on a large scale is still limited. This paper describes the backgrounds of PHAs and discussed the current state of knowledge on the polyhydroxyalkanoates. Ability of bacteria to convert different carbon sources to PHAs, the opportunities and challenges of their introduction to global market as valuable renewable products have been also discussed. Copyright © 2016 Elsevier GmbH. All rights reserved.

  19. Bacterial diversity among four healthcare-associated institutes in Taiwan.

    Science.gov (United States)

    Chen, Chang-Hua; Lin, Yaw-Ling; Chen, Kuan-Hsueh; Chen, Wen-Pei; Chen, Zhao-Feng; Kuo, Han-Yueh; Hung, Hsueh-Fen; Tang, Chuan Yi; Liou, Ming-Li

    2017-08-15

    Indoor microbial communities have important implications for human health, especially in health-care institutes (HCIs). The factors that determine the diversity and composition of microbiomes in a built environment remain unclear. Herein, we used 16S rRNA amplicon sequencing to investigate the relationships between building attributes and surface bacterial communities among four HCIs located in three buildings. We examined the surface bacterial communities and environmental parameters in the buildings supplied with different ventilation types and compared the results using a Dirichlet multinomial mixture (DMM)-based approach. A total of 203 samples from the four HCIs were analyzed. Four bacterial communities were grouped using the DMM-based approach, which were highly similar to those in the 4 HCIs. The α-diversity and β-diversity in the naturally ventilated building were different from the conditioner-ventilated building. The bacterial source composition varied across each building. Nine genera were found as the core microbiota shared by all the areas, of which Acinetobacter, Enterobacter, Pseudomonas, and Staphylococcus are regarded as healthcare-associated pathogens (HAPs). The observed relationship between environmental parameters such as core microbiota and surface bacterial diversity suggests that we might manage indoor environments by creating new sanitation protocols, adjusting the ventilation design, and further understanding the transmission routes of HAPs.

  20. Cognitive and linguistic sources of variance in 2-year-olds’ speech-sound discrimination: a preliminary investigation.

    Science.gov (United States)

    Lalonde, Kaylah; Holt, Rachael Frush

    2014-02-01

    This preliminary investigation explored potential cognitive and linguistic sources of variance in 2-year-olds’ speech-sound discrimination by using the toddler change/ no-change procedure and examined whether modifications would result in a procedure that can be used consistently with younger 2-year-olds. Twenty typically developing 2-year-olds completed the newly modified toddler change/no-change procedure. Behavioral tests and parent report questionnaires were used to measure several cognitive and linguistic constructs. Stepwise linear regression was used to relate discrimination sensitivity to the cognitive and linguistic measures. In addition, discrimination results from the current experiment were compared with those from 2-year-old children tested in a previous experiment. Receptive vocabulary and working memory explained 56.6% of variance in discrimination performance. Performance was not different on the modified toddler change/no-change procedure used in the current experiment from in a previous investigation, which used the original version of the procedure. The relationship between speech discrimination and receptive vocabulary and working memory provides further evidence that the procedure is sensitive to the strength of perceptual representations. The role for working memory might also suggest that there are specific subject-related, nonsensory factors limiting the applicability of the procedure to children who have not reached the necessary levels of cognitive and linguistic development.

  1. Distinct cellular sources of hepoxilin A3 and leukotriene B4 are used to coordinate bacterial-induced neutrophil transepithelial migration.

    Science.gov (United States)

    Pazos, Michael A; Pirzai, Waheed; Yonker, Lael M; Morisseau, Christophe; Gronert, Karsten; Hurley, Bryan P

    2015-02-01

    Neutrophilic infiltration is a leading contributor to pathology in a number of pulmonary disease states, including cystic fibrosis. Hepoxilin A3 (HXA3) is a chemotactic eicosanoid shown to mediate the transepithelial passage of neutrophils in response to infection in several model systems and at multiple mucosal surfaces. Another well-known eicosanoid mediating general neutrophil chemotaxis is leukotriene B4 (LTB4). We sought to distinguish the roles of each eicosanoid in the context of infection of lung epithelial monolayers by Pseudomonas aeruginosa. Using human and mouse in vitro transwell model systems, we used a combination of biosynthetic inhibitors, receptor antagonists, as well as mutant sources of neutrophils to assess the contribution of each chemoattractant in driving neutrophil transepithelial migration. We found that following chemotaxis to epithelial-derived HXA3 signals, neutrophil-derived LTB4 is required to amplify the magnitude of neutrophil migration. LTB4 signaling is not required for migration to HXA3 signals, but LTB4 generation by migrated neutrophils plays a significant role in augmenting the initial HXA3-mediated migration. We conclude that HXA3 and LTB4 serve independent roles to collectively coordinate an effective neutrophilic transepithelial migratory response. Copyright © 2015 by The American Association of Immunologists, Inc.

  2. The U.S. Navy's Global Wind-Wave Models: An Investigation into Sources of Errors in Low-Frequency Energy Predictions

    National Research Council Canada - National Science Library

    Rogers, W

    2002-01-01

    This report describes an investigation to determine the relative importance of various sources of error in the two global-scale models of wind-generated surface waves used operationally by the U.S. Navy...

  3. Investigation and Applications of In-Source Oxidation in Liquid Sampling-Atmospheric Pressure Afterglow Microplasma Ionization (LS-APAG) Source.

    Science.gov (United States)

    Xie, Xiaobo; Wang, Zhenpeng; Li, Yafeng; Zhan, Lingpeng; Nie, Zongxiu

    2017-06-01

    A liquid sampling-atmospheric pressure afterglow microplasma ionization (LS-APAG) source is presented for the first time, which is embedded with both electrospray ionization (ESI) and atmospheric pressure afterglow microplasma ionization (APAG) techniques. This ion source is capable of analyzing compounds with diverse molecule weights and polarities. An unseparated mixture sample was detected as a proof-of-concept, giving complementary information (both polarities and non-polarities) with the two ionization modes. It should also be noted that molecular mass can be quickly identified by ESI with clean and simple spectra, while the structure can be directly studied using APAG with in-source oxidation. The ionization/oxidation mechanism and applications of the LS-APAG source have been further explored in the analysis of nonpolar alkanes and unsaturated fatty acids/esters. A unique [M + O - 3H] + was observed in the case of individual alkanes (C 5 -C 19 ) and complex hydrocarbons mixture under optimized conditions. Moreover, branched alkanes generated significant in-source fragments, which could be further applied to the discrimination of isomeric alkanes. The technique also facilitates facile determination of double bond positions in unsaturated fatty acids/esters due to diagnostic fragments (the acid/ester-containing aldehyde and acid oxidation products) generated by on-line ozonolysis in APAG mode. Finally, some examples of in situ APAG analysis by gas sampling and surface sampling were given as well. Graphical Abstract ᅟ.

  4. Coupling aerosol-cloud-radiative processes in the WRF-Chem model: Investigating the radiative impact of elevated point sources

    Directory of Open Access Journals (Sweden)

    E. G. Chapman

    2009-02-01

    Full Text Available The local and regional influence of elevated point sources on summertime aerosol forcing and cloud-aerosol interactions in northeastern North America was investigated using the WRF-Chem community model. The direct effects of aerosols on incoming solar radiation were simulated using existing modules to relate aerosol sizes and chemical composition to aerosol optical properties. Indirect effects were simulated by adding a prognostic treatment of cloud droplet number and adding modules that activate aerosol particles to form cloud droplets, simulate aqueous-phase chemistry, and tie a two-moment treatment of cloud water (cloud water mass and cloud droplet number to precipitation and an existing radiation scheme. Fully interactive feedbacks thus were created within the modified model, with aerosols affecting cloud droplet number and cloud radiative properties, and clouds altering aerosol size and composition via aqueous processes, wet scavenging, and gas-phase-related photolytic processes. Comparisons of a baseline simulation with observations show that the model captured the general temporal cycle of aerosol optical depths (AODs and produced clouds of comparable thickness to observations at approximately the proper times and places. The model overpredicted SO2 mixing ratios and PM2.5 mass, but reproduced the range of observed SO2 to sulfate aerosol ratios, suggesting that atmospheric oxidation processes leading to aerosol sulfate formation are captured in the model. The baseline simulation was compared to a sensitivity simulation in which all emissions at model levels above the surface layer were set to zero, thus removing stack emissions. Instantaneous, site-specific differences for aerosol and cloud related properties between the two simulations could be quite large, as removing above-surface emission sources influenced when and where clouds formed within the modeling domain. When summed spatially over the finest

  5. Bacterial contaminations of informally marketed raw milk in Ghana ...

    African Journals Online (AJOL)

    Background: Milk has an outstanding nutritional quality but is also an excellent medium for bacterial growth and an important source of bacterial infection when consumed without pasteurization. Objective: To estimate the bacterial health risk of milk consumption in Accra and Kumasi, the twomajor cities in Ghana. Method: A ...

  6. Investigation on a Novel Discontinuous Pulse-Width Modulation Algorithm for Single-phase Voltage Source Rectifier

    DEFF Research Database (Denmark)

    Qu, Hao; Yang, Xijun; Guo, Yougui

    2014-01-01

    Single-phase voltage source converter (VSC) is an important power electronic converter (PEC), including single-phase voltage source inverter (VSI), single-phase voltage source rectifier (VSR), single-phase active power filter (APF) and single-phase grid-connection inverter (GCI). Single-phase VSC...

  7. INVESTIGATION OF THE NUTRITIONAL REQUIREMENTS AND CORRESPONDING CODING GENES OF HYDROCARBON-DEGRADING BACTERIAL STRAINS FOR THE PRODUCTION OF BIOMASS USEFUL IN BIOREMEDIATION OF PETROLEUM POLLUTION

    OpenAIRE

    Attar, Al Zahraa Omar

    2015-01-01

    Petroleum-derived contamination events constitute one of the most dominant sources of environmental deterioration in the industrialized countries. Hydrocarbon compounds are recognized as toxic and carcinogenic organic pollutants and environmentally persistent. Bioremediation efforts aim to confine, restrain and mitigate the magnitude of contamination, in order to prevent additional decline of the environment and to protect all life forms from exposure to hazardous materials. The aim of this p...

  8. Arsenic uptake in bacterial calcite

    Science.gov (United States)

    Catelani, Tiziano; Perito, Brunella; Bellucci, Francesco; Lee, Sang Soo; Fenter, Paul; Newville, Matthew; Rimondi, Valentina; Pratesi, Giovanni; Costagliola, Pilario

    2018-02-01

    Bio-mediated processes for arsenic (As) uptake in calcite were investigated by means of X-ray Diffraction (XRD) and X-ray Absorption Spectroscopy (XAS) coupled with X-ray Fluorescence measurements. The environmental bacterial strain Bacillus licheniformis BD5, sampled at the Bullicame Hot Springs (Viterbo, Central Italy), was used to synthesize calcite from As-enriched growth media. Both liquid and solid cultures were applied to simulate planktonic and biofilm community environments, respectively. Bacterial calcite samples cultured in liquid media had an As enrichment factor (Kd) 50 times higher than that from solid media. The XRD analysis revealed an elongation of the crystal lattice along the c axis (by 0.03 Å) for biogenic calcite, which likely resulted from the substitution of larger arsenate for carbonate in the crystal. The XAS data also showed a clear difference in the oxidation state of sorbed As between bacterial and abiotic calcite. Abiotic chemical processes yielded predominantly As(V) uptake whereas bacterial precipitation processes led to the uptake of both As(III) and As(V). The presence of As(III) in bacterial calcite is proposed to result from subsequent reduction of arsenate to arsenite by bacterial activities. To the best of our knowledge, this is the first experimental observation of the incorporation of As(III) in the calcite crystal lattice, revealing a critical role of biochemical processes for the As cycling in nature.

  9. Arsenic uptake in bacterial calcite

    Energy Technology Data Exchange (ETDEWEB)

    Catelani, Tiziano; Perito, Brunella; Bellucci, Francesco; Lee, Sang Soo; Fenter, Paul; Newville, Matthew G.; Rimondi, Valentina; Pratesi, Giovanni; Costagliola, Pilario

    2018-02-01

    Bio-mediated processes for arsenic (As) uptake in calcite were investigated by means of X-ray Diffraction (XRD) and Xray Absorption Spectroscopy (XAS) coupled with X-ray Fluorescence measurements. The environmental bacterial strain Bacillus licheniformis BD5, sampled at the Bullicame Hot Springs (Viterbo, Central Italy), was used to synthesize calcite from As-enriched growth media. Both liquid and solid cultures were applied to simulate planktonic and biofilm community environments, respectively. Bacterial calcite samples cultured in liquid media had an As enrichment factor (Kd) 50 times higher than that from solid media. The XRD analysis revealed an elongation of the crystal lattice along the c axis (by 0.03Å) for biogenic calcite, which likely resulted from the substitution of larger arsenate for carbonate in the crystal. The XAS data also showed a clear difference in the oxidation state of sorbed As between bacterial and abiotic calcite. Abiotic chemical processes yielded predominantly As(V) uptake whereas bacterial precipitation processes led to the uptake of both As(III) and As(V). The presence of As(III) in bacterial calcite is proposed to result from subsequent reduction of arsenate to arsenite by bacterial activities. To the best of our knowledge, this is the first experimental observation of the incorporation of As(III) in the calcite crystal lattice, revealing a critical role of biochemical processes for the As cycling in nature.

  10. Bacterial Microcompartments

    Energy Technology Data Exchange (ETDEWEB)

    Kerfeld, Cheryl A.; Heinhorst, Sabine; Cannon, Gordon C.

    2010-06-05

    Bacterialmicrocompartments (BMCs) are organelles composed entirely of protein. They promote specific metabolic processes by encapsulatingand colocalizing enzymes with their substrates and cofactors, by protecting vulnerable enzymes in a defined microenvironment, and bysequestering toxic or volatile intermediates. Prototypes of the BMCsare the carboxysomes of autotrophic bacteria. However, structures of similarpolyhedral shape are being discovered in an ever-increasing number of heterotrophic bacteria, where they participate in the utilization ofspecialty carbon and energy sources.Comparative genomics reveals that the potential for this type of compartmentalization is widespread acrossbacterial phyla and suggests that genetic modules encoding BMCs are frequently laterally transferred among bacteria. The diverse functionsof these BMCs suggest that they contribute to metabolic innovation in bacteria in a broad range of environments.

  11. An experimental investigation on reverse-cycle defrosting performance for an air source heat pump using an electronic expansion valve

    International Nuclear Information System (INIS)

    Qu, Minglu; Xia, Liang; Deng, Shiming; Jiang, Yiqiang

    2012-01-01

    Highlights: ► We experimentally studied the defrost performance under two EEV control strategies. ► The two EEV control strategies were fully open and controlled by a DS controller. ► When the EEV was under the DS controller, higher defrosting efficiency was obtained. -- Abstract: When an air source heat pump (ASHP) operates in heating mode, frost can be accumulated on the surface of its finned outdoor coil. Frosting deteriorates the operation and energy efficiency of the ASHP and periodic defrosting becomes necessary. Currently the most widely used standard defrosting method for ASHPs is reverse cycle defrost. On the other hand, electronic expansion valves (EEVs) are commonly used in heat pump/refrigeration systems, including ASHP units, as throttle regulators of refrigerant flow. This paper reports on an experimental investigation of the performance of a reverse cycle defrost operation for a 6.5 kW experimental heating capacity residential ASHP whose outdoor coil had four-parallel refrigerant circuits, and with an EEV as the refrigerant flow throttle regulator. The detailed descriptions of the experimental ASHP unit and the experimental procedures of ASHP are firstly presented. This is followed by reporting the comparative experiments of two control strategies for the EEV to investigate their effects on defrosting performance: the EEV being fully open and the EEV being regulated by a degree of refrigerant superheat (DS) controller. Experimental results revealed that when the EEV was regulated by the DS controller during defrosting, a higher defrosting efficiency and less heat wastage would be resulted in.

  12. Investigation of natural convection in Miniature Neutron Source Reactor of Isfahan by applying the porous media approach

    Energy Technology Data Exchange (ETDEWEB)

    Abbassi, Yasser, E-mail: y.abbassi@mihanmail.ir [Department of Engineering, University of Shahid Beheshti, Tehran (Iran, Islamic Republic of); Asgarian, Shahla [Department of Chemical Engineering, Isfahan University, Tehran (Iran, Islamic Republic of); Ghahremani, Esmaeel; Abbasi, Mohammad [Department of Engineering, University of Shahid Beheshti, Tehran (Iran, Islamic Republic of)

    2016-12-01

    Highlights: • We carried out a CFD study to investigate transient natural convection in MNSR. • We applied porous media approach to simplify the complex core of MNSR. • Method have been verified with experimental data. • Temperature difference between the core inlet and outlet has been obtained. • Flow pattern and temperature distribution have been presented. - Abstract: The small and complex core of Isfahan Miniature Neutron Source Reactor (MNSR) in addition to its large tank makes a parametric study of natural convection difficult to perform in aspects of time and computational resources. In this study, in order to overcome this obstacle the porous media approximation has been used. This numerical technique includes two steps, (a) calculation of porous media variables such as porosity and pressure drops in the core region, (b) simulation of natural convection in the reactor tank by assuming the core region as a porous medium. Simulation has been carried out with ANSYS FLUENT® Academic Research, Release 16.2. The core porous medium resistance factors have been estimated to be, D{sub ij} = 1850 [1/m] and C{sub ij} = 415 [1/m{sup 2}]. Natural Convection simulation with Boussinesq approximation and variable property assumption have been performed. The experimental data and nuclear codes available in the literature, have verified the method. The average temperature difference between the experimental data and this study results was less than 0.5 °C and 2.0 °C for property variable technique and Boussinesq approximation, respectively. Temperature distribution and flow pattern in the entire reactor have been obtained. Results have shown that the temperature difference between core outlet and inlet is about 18°C and in this situation flow rate is about 0.004 kg/s. A full parametric study could be the topic of future investigations.

  13. Experimental investigation on a small pumpless ORC (organic rankine cycle) system driven by the low temperature heat source

    International Nuclear Information System (INIS)

    Gao, P.; Wang, L.W.; Wang, R.Z.; Jiang, L.; Zhou, Z.S.

    2015-01-01

    A small pumpless ORC (organic rankine cycle) system with different scroll expanders modified from compressors of the automobile air-conditioner is established, and the refrigerant R245fa is chosen as the working fluid. Different hot water temperatures of 80, 85, 90 and 95 °C are employed to drive the pumpless ORC system. Experimental results show that a maximum shaft power of 361.0 W is obtained under the hot water temperature of 95 °C, whereas the average shaft power is 155.8 W. The maximum energy efficiency of 2.3% and the maximum exergy efficiency of 12.8% are obtained at the hot water temperature of 90 °C. Meanwhile a test rig for investigating the mechanical loss of the scroll expander is established. The torque caused by the internal mechanical friction of the expander is about 0.4 N m. Additionally, another scroll expander with a displacement of 86 ml/r is also employed to investigate how scroll expander displacement influences the performance of the pumpless ORC system. Finally, the performance of the pumpless ORC system is compared with that of the conventional ORC system, and experimental results show that the small pumpless ORC system has more advantages for the low-grade heat recovery. - Highlights: • A small pumpless ORC (organic rankine cycle) system is established, and different scroll expanders are tested. • The maximum energy and exergy efficiency are 2.3% and 12.8% respectively. • A maximum shaft power of 361.0 W is obtained under the heat source temperature of 95 °C. • The small pumpless ORC system has characteristics of the high efficiency.

  14. Investigating the Sources and Dynamics of Dissolved Organic Matter in an Agricultural Watershed in California (U.S.A.)

    Science.gov (United States)

    Dyda, R. Y.; Hernes, P. J.; Spencer, R. G.; Ingrum, T. D.; Pellerin, B. A.; Bergamaschi, B. A.

    2007-12-01

    base flow, consistent with an increase in terrestrial signature. Biomolecular markers such as lignin phenols provide diagnostic source information on DOM as they are derived uniquely from vascular plants. Lignin can be used to differentiate angiosperm and gymnosperm tissues, but more importantly for this study, carbon-normalized yields can help to constrain the proportions of the increasing DOC during irrigation that come from vascular plants versus in situ production. In addition to supplying useful source information, dissolved lignin phenols undergo rapid photodegradation when exposed to adequate solar radiation, and this process is "imprinted" through increases in the acidic components of lignin and decreases in the syringyl phenols, thus providing insight into DOM cycling in agriculture-dominated watersheds. Data will be presented highlighting the use of a range of analytical and spectrophotometric measurements including lignin phenols, 13C of DOC and CDOM in the Willow Slough watershed for investigating sources and dynamics of DOM throughout the watershed.

  15. Design and investigations of the superconducting magnet system for the multipurpose superconducting electron cyclotron resonance ion source.

    Science.gov (United States)

    Tinschert, K; Lang, R; Mäder, J; Rossbach, J; Spädtke, P; Komorowski, P; Meyer-Reumers, M; Krischel, D; Fischer, B; Ciavola, G; Gammino, S; Celona, L

    2012-02-01

    The production of intense beams of heavy ions with electron cyclotron resonance ion sources (ECRIS) is an important request at many accelerators. According to the ECR condition and considering semi-empirical scaling laws, it is essential to increase the microwave frequency together with the magnetic flux density of the ECRIS magnet system. A useful frequency of 28 GHz, therefore, requires magnetic flux densities above 2.2 T implying the use of superconducting magnets. A cooperation of European institutions initiated a project to build a multipurpose superconducting ECRIS (MS-ECRIS) in order to achieve an increase of the performances in the order of a factor of ten. After a first design of the superconducting magnet system for the MS-ECRIS, the respective cold testing of the built magnet system reveals a lack of mechanical performance due to the strong interaction of the magnetic field of the three solenoids with the sextupole field and the magnetization of the magnetic iron collar. Comprehensive structural analysis, magnetic field calculations, and calculations of the force pattern confirm thereafter these strong interactions, especially of the iron collar with the solenoidal fields. The investigations on the structural analysis as well as suggestions for a possible mechanical design solution are given.

  16. An investigation of the usability of sound recognition for source separation of packaging wastes in reverse vending machines.

    Science.gov (United States)

    Korucu, M Kemal; Kaplan, Özgür; Büyük, Osman; Güllü, M Kemal

    2016-10-01

    In this study, we investigate the usability of sound recognition for source separation of packaging wastes in reverse vending machines (RVMs). For this purpose, an experimental setup equipped with a sound recording mechanism was prepared. Packaging waste sounds generated by three physical impacts such as free falling, pneumatic hitting and hydraulic crushing were separately recorded using two different microphones. To classify the waste types and sizes based on sound features of the wastes, a support vector machine (SVM) and a hidden Markov model (HMM) based sound classification systems were developed. In the basic experimental setup in which only free falling impact type was considered, SVM and HMM systems provided 100% classification accuracy for both microphones. In the expanded experimental setup which includes all three impact types, material type classification accuracies were 96.5% for dynamic microphone and 97.7% for condenser microphone. When both the material type and the size of the wastes were classified, the accuracy was 88.6% for the microphones. The modeling studies indicated that hydraulic crushing impact type recordings were very noisy for an effective sound recognition application. In the detailed analysis of the recognition errors, it was observed that most of the errors occurred in the hitting impact type. According to the experimental results, it can be said that the proposed novel approach for the separation of packaging wastes could provide a high classification performance for RVMs. Copyright © 2016 Elsevier Ltd. All rights reserved.

  17. An investigation of the physiology and potential role of components of the deep ocean bacterial community (of the NE Atlantic) by enrichments carried out under minimal environmental change

    Science.gov (United States)

    Egan, Simon T.; McCarthy, David M.; Patching, John W.; Fleming, Gerard T. A.

    2012-03-01

    Samples of deep-ocean water (3170 m) taken from the Rockall Trough (North-East Atlantic) were incubated for one-month at atmospheric and in-situ pressure (31 MPa), at 4 °C and in the absence and presence of added nutrients. Prokaryotic abundance (direct cell counts) increased by at least 28-fold in enrichments without added nutrients. However, the magnitude of increase in abundance was less for incubations carried out at in-situ pressure (131-181-fold) than those incubations at surface pressure (163-1714-fold increase in abundance). Changes in the prokaryotic community profile as a result of one-month incubation were measured by means of Denaturing Gel Electrophoresis (DGGE) of extracted 16S rDNA. The profiles of post-incubation samples incubated at in-situ pressure were separated from all other profiles as were those of unpressurised samples with added nutrients. The behaviour (fitness) of individual community members (Operational Taxonomic Units: OTUs) was determined on the basis of change in relative DGGE band intensities between pre- and post-incubation samples. Of twenty-one OTUs examined, six were fitter when incubated in the presence of added nutrients and at in-situ pressure and one of these was advantaged when grown in the absence of added nutrients and at in-situ pressure. These represented autochthonous and active members of the deep-ocean prokaryotic community. In contrast, seven OTUs were disadvantaged when grown under in-situ pressure and were indicative surface-derived allochtonous microorganisms. A further two OTUs came to dominance in incubations with added nutrients (pressurised and unpressurised) and similar to the previous category were probably surface-derived microorganisms. A single OTU showed characteristics of piezophilic and oliogrophic behaviour and four OTUs were disadvantaged under all incubation conditions examined. The twenty-one DGGE bands were sequenced and the bacterial communities were dominated by Gamma proteobactria and to a

  18. Bacterial Prostatitis: Bacterial Virulence, Clinical Outcomes, and New Directions.

    Science.gov (United States)

    Krieger, John N; Thumbikat, Praveen

    2016-02-01

    Four prostatitis syndromes are recognized clinically: acute bacterial prostatitis, chronic bacterial prostatitis, chronic prostatitis/chronic pelvic pain syndrome, and asymptomatic prostatitis. Because Escherichia coli represents the most common cause of bacterial prostatitis, we investigated the importance of bacterial virulence factors and antimicrobial resistance in E. coli strains causing prostatitis and the potential association of these characteristics with clinical outcomes. A structured literature review revealed that we have limited understanding of the virulence-associated characteristics of E. coli causing acute prostatitis. Therefore, we completed a comprehensive microbiological and molecular investigation of a unique strain collection isolated from healthy young men. We also considered new data from an animal model system suggesting certain E. coli might prove important in the etiology of chronic prostatitis/chronic pelvic pain syndrome. Our human data suggest that E. coli needs multiple pathogenicity-associated traits to overcome anatomic and immune responses in healthy young men without urological risk factors. The phylogenetic background and accumulation of an exceptional repertoire of extraintestinal pathogenic virulence-associated genes indicate that these E. coli strains belong to a highly virulent subset of uropathogenic variants. In contrast, antibiotic resistance confers little added advantage to E. coli strains in these healthy outpatients. Our animal model data also suggest that certain pathogenic E. coli may be important in the etiology of chronic prostatitis/chronic pelvic pain syndrome through mechanisms that are dependent on the host genetic background and the virulence of the bacterial strain.

  19. Multiple-source tracking: Investigating sources of pathogens, nutrients, and sediment in the Upper Little River Basin, Kentucky, water years 2013–14

    Science.gov (United States)

    Crain, Angela S.; Cherry, Mac A.; Williamson, Tanja N.; Bunch, Aubrey R.

    2017-09-20

    The South Fork Little River (SFLR) and the North Fork Little River (NFLR) are two major headwater tributaries that flow into the Little River just south of Hopkinsville, Kentucky. Both tributaries are included in those water bodies in Kentucky and across the Nation that have been reported with declining water quality. Each tributary has been listed by the Kentucky Energy and Environment Cabinet—Kentucky Division of Water in the 303(d) List of Waters for Kentucky Report to Congress as impaired by nutrients, pathogens, and sediment for contact recreation from point and nonpoint sources since 2002. In 2009, the Kentucky Energy and Environment Cabinet—Kentucky Division of Water developed a pathogen total maximum daily load (TMDL) for the Little River Basin including the SFLR and NFLR Basins. Future nutrient and suspended-sediment TMDLs are planned once nutrient criteria and suspended-sediment protocols have been developed for Kentucky. In this study, different approaches were used to identify potential sources of fecal-indicator bacteria (FIB), nitrate, and suspended sediment; to inform the TMDL process; and to aid in the implementation of effective watershed-management activities. The main focus of source identification was in the SFLR Basin.To begin understanding the potential sources of fecal contamination, samples were collected at 19 sites for densities of FIB (E. coli) in water and fluvial sediment and at 11 sites for Bacteroidales genetic markers (General AllBac, human HF183, ruminant BoBac, canid BacCan, and waterfowl GFD) during the recreational season (May through October) in 2013 and 2014. Results indicated 34 percent of all E. coli water samples (n=227 samples) did not meet the U.S. Environmental Protection Agency 2012 recommended national criteria for primary recreational waters. No criterion currently exists for E. coli in fluvial sediment. By use of the Spearman’s rank correlation test, densities of FIB in fluvial sediments were observed to have a

  20. Investigating the potential of under-utilised plants from the Asteraceae family as a source of natural antimicrobial and antioxidant extracts.

    Science.gov (United States)

    Kenny, O; Smyth, T J; Walsh, D; Kelleher, C T; Hewage, C M; Brunton, N P

    2014-10-15

    Antimicrobial properties of ethanol and water extracts from eight Asteraceae species were investigated against three Gram positive (Staphylococcus aureus, MRSA and Bacillus cereus) and two Gram negative (Escherichia coli and Salmonella typhimurium) bacterial strains. Ethanol extracts from Centaurea scabiosa, Arctium minus, Taraxacum officinale, Centaurea nigra and Cirsium palustre demonstrated antimicrobial activity against strains of S. aureus, MRSA and B. cereus (MIC=187.5-365μg/ml). Ethanol extracts also had higher antioxidant activities and phenolic content demonstrating a link between these compounds and the bioactivity of these extracts. Further investigation into the phenolic content of ethanol extracts using UPLC-MS/MS lead to the identification and quantification of numerous phenolic compounds in all species including; 18 from Cirsium arvense, 16 from Cirsium vulgare, 19 from C. palustre, 15 from C. nigra, 17 from C. scabiosa, 14 from Sonchus asper, 17 from A. minus and 11 from T. officinale. Copyright © 2014 Elsevier Ltd. All rights reserved.

  1. SU-F-T-281: Monte Carlo Investigation of Sources of Dosimetric Discrepancies with 2D Arrays

    International Nuclear Information System (INIS)

    Afifi, M; Deiab, N; El-Farrash, A; Abd El-Hafez, A; Eldib, A; Veltchev, I; Ma, C

    2016-01-01

    Purpose: Intensity modulated radiation therapy (IMRT) poses a number of challenges for properly measuring commissioning data and quality assurance (QA). Understanding the limitations and use of dosimeters to measure these dose distributions is critical to safe IMRT implementation. In this work, we used Monte Carlo simulations to investigate the possible sources of discrepancy between our measurement with 2D array system and our dose calculation using our treatment planning system (TPS). Material and Methods: MCBEAM and MCSIM Monte Carlo codes were used for treatment head simulation and phantom dose calculation. Accurate modeling of a 6MV beam from Varian trilogy machine was verified by comparing simulated and measured percentage depth doses and profiles. Dose distribution inside the 2D array was calculated using Monte Carlo simulations and our TPS. Then Cross profiles for different field sizes were compared with actual measurements for zero and 90° gantry angle setup. Through the analysis and comparison, we tried to determine the differences and quantify a possible angular calibration factor. Results: Minimum discrepancies was seen in the comparison between the simulated and the measured profiles for the zero gantry angles at all studied field sizes (4×4cm"2, 10×10cm"2, 15×15cm"2, and 20×20cm"2). Discrepancies between our measurements and calculations increased dramatically for the cross beam profiles at the 90° gantry angle. This could ascribe mainly to the different attenuation caused by the layer of electronics at the base behind the ion chambers in the 2D array. The degree of attenuation will vary depending on the angle of beam incidence. Correction factors were implemented to correct the errors. Conclusion: Monte Carlo modeling of the 2D arrays and the derivation of angular dependence correction factors will allow for improved accuracy of the device for IMRT QA.

  2. SU-F-T-281: Monte Carlo Investigation of Sources of Dosimetric Discrepancies with 2D Arrays

    Energy Technology Data Exchange (ETDEWEB)

    Afifi, M [Minia Oncology Center, Minia (Egypt); Deiab, N [National Cancer Institute, Cairo (Egypt); El-Farrash, A [Mansoura University, Mansoura, Mansoura (Egypt); Abd El-Hafez, A [National Institute for Standards, Cairo (Egypt); Eldib, A; Veltchev, I; Ma, C [Fox Chase Cancer Center, Philadelphia, PA (United States)

    2016-06-15

    Purpose: Intensity modulated radiation therapy (IMRT) poses a number of challenges for properly measuring commissioning data and quality assurance (QA). Understanding the limitations and use of dosimeters to measure these dose distributions is critical to safe IMRT implementation. In this work, we used Monte Carlo simulations to investigate the possible sources of discrepancy between our measurement with 2D array system and our dose calculation using our treatment planning system (TPS). Material and Methods: MCBEAM and MCSIM Monte Carlo codes were used for treatment head simulation and phantom dose calculation. Accurate modeling of a 6MV beam from Varian trilogy machine was verified by comparing simulated and measured percentage depth doses and profiles. Dose distribution inside the 2D array was calculated using Monte Carlo simulations and our TPS. Then Cross profiles for different field sizes were compared with actual measurements for zero and 90° gantry angle setup. Through the analysis and comparison, we tried to determine the differences and quantify a possible angular calibration factor. Results: Minimum discrepancies was seen in the comparison between the simulated and the measured profiles for the zero gantry angles at all studied field sizes (4×4cm{sup 2}, 10×10cm{sup 2}, 15×15cm{sup 2}, and 20×20cm{sup 2}). Discrepancies between our measurements and calculations increased dramatically for the cross beam profiles at the 90° gantry angle. This could ascribe mainly to the different attenuation caused by the layer of electronics at the base behind the ion chambers in the 2D array. The degree of attenuation will vary depending on the angle of beam incidence. Correction factors were implemented to correct the errors. Conclusion: Monte Carlo modeling of the 2D arrays and the derivation of angular dependence correction factors will allow for improved accuracy of the device for IMRT QA.

  3. Investigating Ozone Sources in California Using AJAX Airborne Measurements and Models: Implications for Stratospheric Intrusion and Long Range Transport

    Science.gov (United States)

    Ryoo, Ju-Mee; Johnson, Matthew S.; Iraci, Laura T.; Yates, Emma L.; Pierce, R. Bradley; Tanaka, Tomoaki; Gore, Warren

    2016-01-01

    High ozone concentrations at low altitudes near the surface were detected from airborne Alpha Jet Atmospheric eXperiment (AJAX) measurements on May 30, 2012. We investigate the causes of the elevated ozone concentrations using the airborne measurements and various models. GEOSchem and WRF-STILT model simulations show that the contribution from local sources is small. From MERRA reanalysis, it is found that high potential vorticity (PV) is observed at low altitudes. This high PV appears to be only partially coming through the stratospheric intrusions because the air inside the high PV region is moist, which shows that mixing appears to be enhanced in the low altitudes. Considering that diabatic heating can also produce high PV in the lower troposphere, high ozone is partially coming through stratospheric intrusion, but this cannot explain the whole ozone concentration in the target areas of the western U.S. A back-trajectory model is utilized to see where the air masses originated. The air masses of the target areas came from the lower stratosphere (LS), upper (UT), mid- (MT), and lower troposphere (LT). The relative number of trajectories coming from LS and UT is low (7.7% and 7.6%, respectively) compared to that from LT (64.1%), but the relative ozone concentration coming from LS and UT is high (38.4% and 20.95%, respectively) compared to that from LT (17.7%). The air mass coming from LT appears to be mostly coming from Asia. Q diagnostics show that there is sufficient mixing along the trajectory to indicate that ozone from the different origins is mixed and transported to the western U.S. This study shows that high ozone concentrations can be detected by airborne measurements, which can be analyzed by integrated platforms such as models, reanalysis, and satellite data.

  4. Investigation of Ozone Sources in California Using AJAX Airborne Measurements and Models: Implications for Stratospheric Intrusion and Long Range Transport

    Science.gov (United States)

    Ryoo, Ju-Mee; Johnson, Matthew S.; Iraci, Laura T.; Yates, Emma L.; Pierce, R. Bradley; Tanaka, Tomoaki; Gore, Warren

    2015-01-01

    High ozone concentrations at low altitudes near the surface were detected from airborne Alpha Jet Atmospheric eXperiment (AJAX) measurements on May 30, 2012. We investigate the causes of the elevated ozone concentrations using the airborne measurements and various models. GEOS-chem and WRF-STILT model simulations show that the contribution from local sources is small. From MERRA reanalysis, it is found that high potential vorticity (PV) is observed at low altitudes. This high PV appears to be only partially coming through the stratospheric intrusions because the air inside the high PV region is moist, which shows that mixing appears to be enhanced in the low altitudes. Considering that diabatic heating can also produce high PV in the lower troposphere, high ozone is partially coming through stratospheric intrusion, but this cannot explain the whole ozone concentration in the target areas of the western U.S. A back-trajectory model is utilized to see where the air masses originated. The air masses of the target areas came from the lower stratosphere (LS), upper (UT), mid- (MT), and lower troposphere (LT). The relative number of trajectories coming from LS and UT is low (7.7 and 7.6, respectively) compared to that from LT (64.1), but the relative ozone concentration coming from LS and UT is high (38.4 and 20.95, respectively) compared to that from LT (17.7). The air mass coming from LT appears to be mostly coming from Asia. Q diagnostics show that there is sufficient mixing along the trajectory to indicate that ozone from the different origins is mixed and transported to the western U.S. This study shows that high ozone concentrations can be detected by airborne measurements, which can be analyzed by integrated platforms such as models, reanalysis, and satellite data.

  5. Systematic overview finds variation in approaches to investigating and reporting on sources of heterogeneity in systematic reviews of diagnostic studies

    NARCIS (Netherlands)

    Naaktgeboren, Christiana A.; van Enst, Wynanda A.; Ochodo, Eleanor A.; de Groot, Joris A. H.; Hooft, Lotty; Leeflang, Mariska M.; Bossuyt, Patrick M.; Moons, Karel G. M.; Reitsma, Johannes B.

    2014-01-01

    To examine how authors explore and report on sources of heterogeneity in systematic reviews of diagnostic accuracy studies. A cohort of systematic reviews of diagnostic tests was systematically identified. Data were extracted on whether an exploration of the sources of heterogeneity was undertaken,

  6. Source investigation of the tar balls deposited along the Gujarat coast, India, using chemical fingerprinting and transport modeling techniques

    Digital Repository Service at National Institute of Oceanography (India)

    Suneel, V.; Vethamony, P.; Naik, B.G.; VinodKumar, K.; Sreenu, L.; Samiksha, S.V.; Tai,Y.; Sudheesh, K.

    –1339. 14    (6). Brandli, M., Bucheli, T.D., Kupper, T., Mayer, J., Stadelman, F.X., Taradellas, J. Fate of PCBs, PAHs and their source characteristic ratios during composting and digestion of source-separated organic waste in full-scale plants. Environ...

  7. Investigation of the problems associated with the Pt-20 Rh clads of the viking heat sources VF-3 and VF-7

    International Nuclear Information System (INIS)

    Zielinski, R.E.

    1975-01-01

    The investigation was undertaken to determine why the emissive coatings of the Viking Heat Sources VF-3 and VF-7 had flaked off the Pt-20 Rh clad material. During the course of the investigation, two additional, unusual phenomena were observed: a surface reaction zone on the uncoated Pt-20 Rh surfaces and grain boundary reactions in the weld zone of the clad. It seems that all three phenomena were the result of a complicated reaction couple that involved the heat source materials, storage container materials, and environment. Vapor transport mechanisms were responsible for the phenomena which were observed. The vapor transport mechanisms were probably initiated because of the impurities that were in both the heat source and storage container materials. The mechanisms resulted in significant changes in these heat sources because vapor transport was allowed to continue in a static environment for an unusually long period of time. (U.S.)

  8. Investigation of Anisotropy Caused by Cylinder Applicator on Dose Distribution around Cs-137 Brachytherapy Source using MCNP4C Code

    Directory of Open Access Journals (Sweden)

    Sedigheh Sina

    2011-06-01

    Full Text Available Introduction: Brachytherapy is a type of radiotherapy in which radioactive sources are used in proximity of tumors normally for treatment of malignancies in the head, prostate and cervix. Materials and Methods: The Cs-137 Selectron source is a low-dose-rate (LDR brachytherapy source used in a remote afterloading system for treatment of different cancers. This system uses active and inactive spherical sources of 2.5 mm diameter, which can be used in different configurations inside the applicator to obtain different dose distributions. In this study, first the dose distribution at different distances from the source was obtained around a single pellet inside the applicator in a water phantom using the MCNP4C Monte Carlo code. The simulations were then repeated for six active pellets in the applicator and for six point sources.  Results: The anisotropy of dose distribution due to the presence of the applicator was obtained by division of dose at each distance and angle to the dose at the same distance and angle of 90 degrees. According to the results, the doses decreased towards the applicator tips. For example, for points at the distances of 5 and 7 cm from the source and angle of 165 degrees, such discrepancies reached 5.8% and 5.1%, respectively.  By increasing the number of pellets to six, these values reached 30% for the angle of 5 degrees. Discussion and Conclusion: The results indicate that the presence of the applicator causes a significant dose decrease at the tip of the applicator compared with the dose in the transverse plane. However, the treatment planning systems consider an isotropic dose distribution around the source and this causes significant errors in treatment planning, which are not negligible, especially for a large number of sources inside the applicator.

  9. Neutron source investigations in support of the cross section program at the Argonne Fast-Neutron Generator

    International Nuclear Information System (INIS)

    Meadows, J.W.; Smith, D.L.

    1980-05-01

    Experimental methods related to the production of neutrons for cross section studies at the Argonne Fast-Neutron Generator are reviewed. Target assemblies commonly employed in these measurements are described, and some of the relevant physical properties of the neutron source reactions are discussed. Various measurements have been performed to ascertain knowledge about these source reaction that is required for cross section data analysis purposes. Some results from these studies are presented, and a few specific examples of neutron-source-related corrections to cross section data are provided. 16 figures, 3 tables

  10. Airborne bacterial assemblage in a zero carbon building: A case study.

    Science.gov (United States)

    Leung, M H Y; Tong, X; Tong, J C K; Lee, P K H

    2018-01-01

    Currently, there is little information pertaining to the airborne bacterial communities of green buildings. In this case study, the air bacterial community of a zero carbon building (ZCB) in Hong Kong was characterized by targeting the bacterial 16S rRNA gene. Bacteria associated with the outdoor environment dominated the indoor airborne bacterial assemblage, with a modest contribution from bacteria associated with human skin. Differences in overall community diversity, membership, and composition associated with short (day-to-day) and long-term temporal properties were detected, which may have been driven by specific environmental genera and taxa. Furthermore, time-decay relationships in community membership (based on unweighted UniFrac distances) and composition (based on weighted UniFrac distances) differed depending on the season and sampling location. A Bayesian source-tracking approach further supported the importance of adjacent outdoor air bacterial assemblage in sourcing the ZCB indoor bioaerosol. Despite the unique building attributes, the ZCB microbial assemblage detected and its temporal characteristics were not dissimilar to that of conventional built environments investigated previously. Future controlled experiments and microbial assemblage investigations of other ZCBs will undoubtedly uncover additional knowledge related to how airborne bacteria in green buildings may be influenced by their distinctive architectural attributes. © 2017 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

  11. Investigation of the ion beam of the Titan source by the time-of-flight mass spectrometer

    International Nuclear Information System (INIS)

    Bugaev, A.S.; Gushenets, V.V.; Nikolaev, A.G.; Yushkov, G.Yu.

    2000-01-01

    The Titan ion source generates wide-aperture beams of both gaseous and metal ions of various materials. The above possibility is realized on the account of combining two types of arc discharge with cold cathodes in the source discharge system. The vacuum arc, initiated between the cathode accomplished from the ion forming material, and hollow anode, is used for obtaining the metal ions. The pinch-effect low pressure arc discharge, ignited on the same hollow anode, is used for obtaining gaseous ions. The composition of ion beams, generated by the Titan source through the specially designed time-of-flight spectrometer, is studied. The spectrometer design and principle pf operation are presented. The physical peculiarities of the source functioning, influencing the ion beam composition, are discussed [ru

  12. Source investigation of two outbreaks of skin and soft tissue infection by Mycobacterium abscessus subsp. abscessus in Venezuela.

    Science.gov (United States)

    Torres-Coy, J A; Rodríguez-Castillo, B A; Pérez-Alfonzo, R; DE Waard, J H

    2016-04-01

    Outbreaks of soft tissue or skin infection due to non-tuberculous mycobacteria are reported frequently in scientific journals but in general the infection source in these outbreaks remains unknown. In Venezuela, in two distinct outbreaks, one after breast augmentation surgery and another after hydrolipoclasy therapy, 16 patients contracted a soft tissue infection due to Mycobacterium abscessus subsp. abscessus. Searching for the possible environmental infection sources in these outbreaks, initially the tap water (in the hydrolipoclasy therapy outbreak) and a surgical skin marker (in the breast implant surgery outbreak), were identified as the infection sources. Molecular typing of the strains with a variable number tandem repeat typing assay confirmed the tap water as the infection source but the molecular typing technique excluded the skin marker. We discuss the results and make a call for the implementation of stringent hygiene and disinfection guidelines for cosmetic procedures in Venezuela.

  13. Attributing human foodborne illness to food sources and water in Latin America and the Caribbean using data from outbreak investigations

    DEFF Research Database (Denmark)

    Pires, Sara Monteiro; Vieira, Antonio; Perez, Enrique

    2012-01-01

    &C). Foods implicated in outbreaks were classified by their ingredients as simple foods (i.e. belonging to one single food category), or complex foods (i.e. belonging to multiple food categories). For each agent, the data from simple-food outbreaks were summarized, and the proportion of outbreaks caused...... by each category was used to define the probability that an outbreak was caused by a source. For the calculation of the number of outbreaks attributed to each source, simple-food outbreaks were attributed to the single food category in question, and complex-food outbreaks were partitioned to each category......Foodborne pathogens are responsible for an increasing burden of disease worldwide. Knowledge on the contribution of different food sources and water for disease is essential to prioritize food safety interventions and implement appropriate control measures. Source attribution using outbreak data...

  14. An Investigation of Faculty Relationships as a Source of Motivation for STEM Undergraduates at a Historically Black University

    Science.gov (United States)

    Mitchell, Aquila

    2011-12-01

    The expansion of STEM education and career opportunities among underrepresented populations is a national priority. Therefore, more research is needed that examines the institutional, instructional and individual factors related to African American students' success in these fields. This dissertation study was drawn from a larger mixed-methods longitudinal study (Freeman and Winston, 2007). It utilized a concurrent embedded design of mixed methods (Creswell, 2009), to investigate faculty relationships as a source of motivation for STEM undergraduates at a HBCU. Bandura's (1986) social cognitive theory was the theoretical basis for this study. The following research questions were examined: (a) What is the nature of student-faculty relationships among STEM undergraduates attending an HBCU; (b) How does the nature of faculty student relationships vary by gender and STEM major; (c) How do student-faculty relationships influence students' persistence in STEM, self-concept of ability in mathematics and in science and grade point average; and (d) Does the influence of student-faculty relationships on self-concept of ability in mathematics and in science and grade point average vary by gender and STEM major? Freshman college students (N=167) who had a declared major in STEM fall 2009 were participants in this study. Students were predominately Black/African American (82%) and predominately female (71%). The Student-Professor Interaction Scale (SPIS) was used to measure various dimensions of student-faculty interactions (Cokley, Komarraju, Rosales, et al., 2006). The Experiences with Faculty Scale (Pace & Kuh, 1998) was used to measure the frequency of student's experiences with faculty. Self-Concept of Ability Scales in Mathematics and in Science (Marsh, 1999) was used to measure students' global perception of their abilities. An open-ended question was designed to expand and provide breadth to the quantitative results. Findings indicated that student

  15. Risk of bacterial cross infection associated with inspiration through flow-based spirometers.

    Science.gov (United States)

    Bracci, Massimo; Strafella, Elisabetta; Croce, Nicola; Staffolani, Sara; Carducci, Annalaura; Verani, Marco; Valentino, Matteo; Santarelli, Lory

    2011-02-01

    Bacterial contamination of spirometers has been documented in water-sealed devices, mouthpieces, and connection tubes. Little information is available about bacterial contamination of flow-based apparatuses such as turbine-type spirometers and pneumotachographs. Inspiration through contaminated equipment is a potential source of cross infection. To investigate bacteria mobilization (ie, bacteria detachment and aerosolization from the instrument) during routine spirometric testing, 2 types of flow-based spirometers were used. Bacteria mobilization during artificial inspiration through in-line filters or cardboard mouthpieces was evaluated. Nine hundred workers undergoing periodic spirometric testing were enrolled at the occupational physician office in 30 sessions of 30 subjects each. The participants were asked to perform a forced vital capacity test in a turbine-type spirometer and in an unheated pneumotachograph fitted with disposable in-line filters or cardboard mouthpieces. To evaluate bacterial mobilization, an artificial inspiration was performed and bacterial growth determined. The bacterial growth analysis was assessed after the first and the thirtieth spirometric tests of each session without disinfecting the instruments between tests. In addition, instrument bacterial contamination was evaluated. No significant bacterial mobilization and instrument contamination were found in spirometric tests executed with in-line filters. Conversely, a significant bacterial mobilization and instrument contamination were observed in tests performed with cardboard mouthpieces. Differences between the 2 spirometers were not significant. In-line filters may effectively reduce the risk of bacterial cross infection. Inspiration through flow-based spirometers fitted with disposable cardboard mouthpieces is completely safe when combined with spirometer disinfection/sterilization between subjects. Copyright © 2011 Association for Professionals in Infection Control and

  16. Bacterial Diversity in the Digestive Tracts of Four Indian Air-Breathing Fish Species Investigated by PCR Based Denaturing Gradient Gel Electrophoresis

    Directory of Open Access Journals (Sweden)

    Suxu He

    Full Text Available ABSTRACT An investigation was conducted to identify the allochthonous microbiota (entire intestine and the autochthonous microbiota in proximal intestine (PI and distal intestine (DI of four species of Indian air-breathing fish (climbing perch; Anabas testudineus, murrel; Channa punctatus, walking catfish; Clarias batrachus and stinging catfish; Heteropneustes fossilis by PCR based denaturing gradient gel electrophoresis (DGGE. High similarities of the allochthonous microbiota were observed between climbing perch and murrel, walking catfish and stinging catfish, indicating similar food behavior. The autochthonous microbiota of PI and DI from climbing perch and murrel revealed more similarity, than the result obtained from walking catfish and stinging catfish. The autochthonous microbiota of climbing perch and murrel were similar with regard to the allochthonous microbiota, but no such similarity was observed in case of walking catfish and stinging catfish. The fish genotype and intestinal bacteria are well matched and show co-evolutionary relationship. Three fish species has its unique bacteria; autochthonous Enterobacter cloacae, Edwardsiella tarda and Sphingobium sp. in DI of climbing perch, Pseudomonas sp.; allochthonous and autochthonous in PI of walking catfish and uncultured bacterium (EU697160.1, uncultured bacterium (JF018065.1 and uncultured bacterium (EU697160.1 for stinging catfish. In murrel, no unique bacteria were detected.

  17. Isolation of bacterial cellulose nanocrystalline from pineapple peel waste: Optimization of acid concentration in the hydrolysis method

    Science.gov (United States)

    Anwar, Budiman; Rosyid, Nurul Huda; Effendi, Devi Bentia; Nandiyanto, Asep Bayu Dani; Mudzakir, Ahmad; Hidayat, Topik

    2016-02-01

    Isolation of needle-shaped bacterial cellulose nanocrystalline with a diameter of 16-64 nm, a fiber length of 258-806 nm, and a degree of crystallinity of 64% from pineapple peel waste using an acid hydrolysis process was investigated. Experimental showed that selective concentration of acid played important roles in isolating the bacterial cellulose nanocrystalline from the cellulose source. To achieve the successful isolation of bacterial cellulose nanocrystalline, various acid concentrations were tested. To confirm the effect of acid concentration on the successful isolation process, the reaction conditions were fixed at a temperature of 50°C, a hydrolysis time of 30 minutes, and a bacterial cellulose-to-acid ratio of 1:50. Pineapple peel waste was used as a model for a cellulose source because to the best of our knowledge, there is no report on the use of this raw material for producing bacterial cellulose nanocrystalline. In fact, this material can be used as an alternative for ecofriendly and cost-free cellulose sources. Therefore, understanding in how to isolate bacterial cellulose nanocrystalline from pineapple peel waste has the potential for large-scale production of inexpensive cellulose nanocrystalline.

  18. Heme Synthesis and Acquisition in Bacterial Pathogens

    OpenAIRE

    Choby, Jacob E.; Skaar, Eric P.

    2016-01-01

    Bacterial pathogens require the iron-containing cofactor heme to cause disease. Heme is essential to the function of hemoproteins, which are involved in energy generation by the electron transport chain, detoxification of host immune effectors, and other processes. During infection, bacterial pathogens must synthesize heme or acquire heme from the host; however, host heme is sequestered in high-affinity hemoproteins. Pathogens have evolved elaborate strategies to acquire heme from host source...

  19. Investigation and optimisation of mobile NaI(Tl) and 3He-based neutron detectors for finding point sources

    International Nuclear Information System (INIS)

    Nilsson, Jonas M.C.; Finck, Robert R.; Rääf, Christopher

    2015-01-01

    Neutron radiation produces high-energy gamma radiation through (n,γ) reactions in matter. This can be used to detect neutron sources indirectly using gamma spectrometers. The sensitivity of a gamma spectrometer to neutrons can be amplified by surrounding it with polyvinyl chloride (PVC). The hydrogen in the PVC acts as a moderator and the chlorine emits prompt gammas when a neutron is captured. A 4.7-l 3 He-based mobile neutron detector was compared to a 4-l NaI(Tl)-detector covered with PVC using this principle. Methods were also developed to optimise the measurement parameters of the systems. The detector systems were compared with regard to their ability to find 241 AmBe, 252 Cf and 238 Pu– 13 C neutron sources. Results from stationary measurements were used to calculate optimal integration times as well as minimum detectable neutron emission rates. It was found that the 3 He-based detector was more sensitive to 252 Cf sources whereas the NaI(Tl) detector was more sensitive to 241 AmBe and 238 Pu– 13 C sources. The results also indicated that the sensitivity of the detectors to sources at known distances could theoretically be improved by 60% by changing from fixed integration times to list mode in mobile surveys

  20. College Students' Health Information Activities on Facebook: Investigating the Impacts of Health Topic Sensitivity, Information Sources, and Demographics.

    Science.gov (United States)

    Syn, Sue Yeon; Kim, Sung Un

    2016-07-01

    College students tend to lack access to health information. Because social networking sites (SNSs) are popularly adopted by college students, SNSs are considered to be good media channels for college students to obtain health-related information. This study examines the factors that influence college students' health information-seeking and -sharing activities on Facebook. An online survey was distributed to college students between the ages of 18 and 29 to determine intentions pertaining to health information activities according to the factors identified for the study. The factors included both contextual factors (such as health topic sensitivity and health information sources) as well as user factors (such as demographics). Our findings showed that college students are willing to read and post health-related information on Facebook when the health topic is not sensitive. In addition, there are clear differences in preferences between professional sources and personal sources as health information sources. It was found that most user factors, except gender, have no influence on health information activities. The impacts of SNS contexts, awareness of information sources, types of interlocutors, and privacy concerns are further discussed.

  1. Sources of particulate matter components in the Athabasca oil sands region: investigation through a comparison of trace element measurement methodologies

    Science.gov (United States)

    Phillips-Smith, Catherine; Jeong, Cheol-Heon; Healy, Robert M.; Dabek-Zlotorzynska, Ewa; Celo, Valbona; Brook, Jeffrey R.; Evans, Greg

    2017-08-01

    The province of Alberta, Canada, is home to three oil sands regions which, combined, contain the third largest deposit of oil in the world. Of these, the Athabasca oil sands region is the largest. As part of Environment and Climate Change Canada's program in support of the Joint Canada-Alberta Implementation Plan for Oil Sands Monitoring program, concentrations of trace elements in PM2. 5 (particulate matter smaller than 2.5 µm in diameter) were measured through two campaigns that involved different methodologies: a long-term filter campaign and a short-term intensive campaign. In the long-term campaign, 24 h filter samples were collected once every 6 days over a 2-year period (December 2010-November 2012) at three air monitoring stations in the regional municipality of Wood Buffalo. For the intensive campaign (August 2013), hourly measurements were made with an online instrument at one air monitoring station; daily filter samples were also collected. The hourly and 24 h filter data were analyzed individually using positive matrix factorization. Seven emission sources of PM2. 5 trace elements were thereby identified: two types of upgrader emissions, soil, haul road dust, biomass burning, and two sources of mixed origin. The upgrader emissions, soil, and haul road dust sources were identified through both the methodologies and both methodologies identified a mixed source, but these exhibited more differences than similarities. The second upgrader emissions and biomass burning sources were only resolved by the hourly and filter methodologies, respectively. The similarity of the receptor modeling results from the two methodologies provided reassurance as to the identity of the sources. Overall, much of the PM2. 5-related trace elements were found to be anthropogenic, or at least to be aerosolized through anthropogenic activities. These emissions may in part explain the previously reported higher levels of trace elements in snow, water, and biota samples collected

  2. Water Sources and Their Protection from the Impact of Microbial Contamination in Rural Areas of Beijing, China

    Directory of Open Access Journals (Sweden)

    Hairong Li

    2013-03-01

    Full Text Available Bacterial contamination of drinking water is a major public health problem in rural China. To explore bacterial contamination in rural areas of Beijing and identify possible causes of bacteria in drinking water samples, water samples were collected from wells in ten rural districts of Beijing, China. Total bacterial count, total coliforms and Escherichia coli in drinking water were then determined and water source and wellhead protection were investigated. The bacterial contamination in drinking water was serious in areas north of Beijing, with the total bacterial count, total coliforms and Escherichia coli in some water samples reaching 88,000 CFU/mL, 1,600 MPN/100 mL and 1,600 MPN/100 mL, respectively. Water source types, well depth, whether the well was adequately sealed and housed, and whether wellhead is above or below ground were the main factors influencing bacterial contamination levels in drinking water. The bacterial contamination was serious in the water of shallow wells and wells that were not closed, had no well housing or had a wellhead below ground level. The contamination sources around wells, including village dry toilets and livestock farms, were well correlated with bacterial contamination. Total bacterial counts were affected by proximity to sewage ditches and polluting industries, however, proximity to landfills did not influence the microbial indicators.

  3. One, Two, Three, Four, Nothing More: An Investigation of the Conceptual Sources of the Verbal Counting Principles

    Science.gov (United States)

    Le Corre, Mathieu; Carey, Susan

    2007-01-01

    Since the publication of [Gelman, R., & Gallistel, C. R. (1978). "The child's understanding of number." Cambridge, MA: Harvard University Press.] seminal work on the development of verbal counting as a representation of number, the nature of the ontogenetic sources of the verbal counting principles has been intensely debated. The present…

  4. Investigation of black and brown carbon multiple-wavelength-dependent light absorption from biomass and fossil fuel combustion source emissions

    Science.gov (United States)

    Michael R. Olson; Mercedes Victoria Garcia; Michael A. Robinson; Paul Van Rooy; Mark A. Dietenberger; Michael Bergin; James Jay Schauer

    2015-01-01

    Quantification of the black carbon (BC) and brown carbon (BrC) components of source emissions is critical to understanding the impact combustion aerosols have on atmospheric light absorption. Multiple-wavelength absorption was measured from fuels including wood, agricultural biomass, coals, plant matter, and petroleum distillates in controlled combustion settings....

  5. Free and Open Source Tools (FOSTs): An Empirical Investigation of Pre-Service Teachers' Competencies, Attitudes, and Pedagogical Intentions

    Science.gov (United States)

    Asing-Cashman, Joyce G.; Gurung, Binod; Limbu, Yam B.; Rutledge, David

    2014-01-01

    This study examines the digital native pre-service teachers' (DNPSTs) perceptions of their competency, attitude, and pedagogical intention to use free and open source tools (FOSTs) in their future teaching. Participants were 294 PSTs who responded to pre-course surveys at the beginning of an educational technology course. Using the structural…

  6. Investigation of the present management status of calibration source based on the law concerning prevention of radiation hazards due to radioisotopes

    International Nuclear Information System (INIS)

    Takahashi, Yasuyuki; Igarashi, Hiroshi; Hirano, Kunihiro; Kawaharada, Yasuhiro; Igarashi, Hitoshi; Murase, Ken-ya; Mochizuki, Teruhito

    2007-01-01

    An amendment concerning the enforcement of the law on the prevention of radiation hazards due to radioisotopes, etc., and the medical service law enforcement regulations were promulgated on June 1, 2005. This amendment concerned international basic safety standards and the sealing of radiation sources. Sealed radiation sources ≤3.7 MBq, which had been excluded from regulation, were newly included as an object of regulation. Investigation of the single photon emission computed tomography (SPECT) system instituted in hospitals indicated that almost all institutions adhere to the new amendment, and the calibration source, the checking source, etc., corresponding to this amendment were maintained appropriately. Any institutions planning to return sealed radioisotopes should refer to this report. (author)

  7. AN EMPIRICAL INVESTIGATION OF THE RELATIONSHIP BETWEEN DEMOGRAPHICS AND THE USAGE AND PERCEIVED CREDIBILITY OF SOURCES OF INFORMATION ON ACCOMMODATION PROVIDERS

    Directory of Open Access Journals (Sweden)

    Ovidiu I. MOISESCU

    2013-11-01

    Full Text Available The purpose of this paper is to investigate the relationship between the usage and perceived credibility of several sources of information domestic travelers take into consideration when gathering information on potential accommodation providers, on one hand, and, respectively, travelers’ demographic characteristics, on the other hand. After analyzing data from an online questionnaire based study conducted among a sample of 346 young Romanian Facebook users (between 19 and 35 years old, the results showed that, considering types of information sources usually taken into consideration, personal sources and Facebook are more frequently found among travelers with a lower income, travel agencies are more frequently mentioned as usual sources of information among older travelers and among those with a higher level of education, while women are more inclined than men to use leaflets and booklets as sources of information on accommodation providers. Moreover, the research showed that the higher the income, the higher the level of perceived credibility of online portals is. Also, travel agencies and personal sources are more frequently mentioned among the most credible sources by women, than by men, while men are slightly more confident than women in online banners and blogs. The findings can be very useful and relevant from a practical perspective, especially for communication and promotion purposes in the hospitality industry.

  8. Investigation of carbonyl compound sources at a rural site in the Yangtze River Delta region of China.

    Science.gov (United States)

    Wang, Ming; Chen, Wentai; Shao, Min; Lu, Sihua; Zeng, Limin; Hu, Min

    2015-02-01

    Carbonyl compounds are important intermediates in atmospheric photochemistry, but their primary sources are still not understood well. In this work, carbonyls, hydrocarbons, and alkyl nitrates were continuously measured during November 2011 at a rural site in the Yangtze River Delta region of China. Mixing ratios of carbonyls and hydrocarbons showed large fluctuations during the entire measurement. The average level for total measured volatile organic compounds during the pollution episode from 25th to 27th November, 2011 was 91.6 ppb, about 7 times the value for the clean period of 7th-8th, November, 2011. To preliminarily identify toluene sources at this site, the emission ratio of toluene to benzene (T/B) during the pollution episode was determined based on photochemical ages derived from the relationship of alkyl nitrates to their parent alkanes. The calculated T/B was 5.8 ppb/ppb, significantly higher than the values of 0.2-1.7 ppb/ppb for vehicular exhaust and other combustion sources, indicating the dominant influence of industrial emissions on ambient toluene. The contributions of industrial sources to ambient carbonyls were then calculated using a multiple linear regression fit model that used toluene and alkyl nitrates as respective tracers for industrial emission and secondary production. During the pollution episode, 18.5%, 69.0%, and 52.9% of measured formaldehyde, acetaldehyde, and acetone were considered to be attributable to industrial emissions. The emission ratios relative to toluene for formaldehyde, acetaldehyde, and acetone were determined to be 0.10, 0.20 and 0.40 ppb/ppb, respectively. More research on industrial carbonyl emission characteristics is needed to understand carbonyl sources better. Copyright © 2014. Published by Elsevier B.V.

  9. Intake of total, animal and plant proteins, and their food sources in 10 countries in the European Prospective Investigation into Cancer and Nutrition

    NARCIS (Netherlands)

    Halkjaer, J.; Olsen, A.; Bjerregaard, L. J.; Deharveng, G.; Tjonneland, A.; Welch, A. A.; Crowe, F. L.; Wirfalt, E.; Hellstrom, V.; Niravong, M.; Touvier, M.; Linseisen, J.; Steffen, A.; Ocke, M. C.; Peeters, P. H. M.; Chirlaque, M. D.; Larranaga, N.; Ferrari, P.; Contiero, P.; Frasca, G.; Engeset, D.; Lund, E.; Misirli, G.; Kosti, M.; Riboli, E.; Slimani, N.; Bingham, S.

    Objective: To describe dietary protein intakes and their food sources among 27 redefined centres in 10 countries participating in the European Prospective Investigation into Cancer and Nutrition (EPIC). Methods: Between 1995 and 2000, 36 034 persons, aged between 35 and 74 years, were administered a

  10. The Roles of Self-Disclosure, Modesty, and Self-Monitoring in the Mentoring Relationship: A Longitudinal Multi-Source Investigation

    Science.gov (United States)

    Blickle, Gerhard; Schneider, Paula B.; Perrewe, Pamela L.; Blass, Fred R.; Ferris, Gerald R.

    2008-01-01

    Purpose: The purpose of this study was to investigate the role of protege self-presentation by self-disclosure, modesty, and self-monitoring in mentoring. Design/methodology/approach: This study used three data sources (i.e. employees, peers, and mentors) and a longitudinal design over a period of two years. Findings: Employee self-disclosure and…

  11. Annual performance investigation and economic analysis of heating systems with a compression-assisted air source absorption heat pump

    International Nuclear Information System (INIS)

    Wu, Wei; Shi, Wenxing; Wang, Baolong; Li, Xianting

    2015-01-01

    Highlights: • Optimal compression ratio of CASAHP is obtained for the maximum energy saving rate. • Annual performance is improved by 10–20% compared to ASAHP without compression. • Energy saving rate is 17.7–29.2% and investment is reduced to 30–60% for CASAHP. • Both compression and partial-design enhance the economy with given energy saving. • Payback time is reduced from 12–32 to 3–6 years by compression and partial-design. - Abstract: The compression-assisted air source absorption heat pump (CASAHP) is a promising alternative heating system in severe operating conditions. In this research, parameter studies on the annual performance under various compression ratios (CRs) and source temperatures are performed to achieve the maximum energy saving rates (ESRs). Economic analyses of the CASAHP under different CRs and partial-design ratios are conducted to obtain an optimal design that considers both energy savings and economy improvements. The results show that the optimal CR becomes higher in colder regions and with lower heat source temperatures. For a source temperature of 130 °C, the optimal CR values in all of the cities are within 2.0. For source temperatures from 100 to 130 °C, the maximum ESR is in the range of 17.7–29.2% in the studied cities. The efficiency improvement rate (EIR) caused by compression in a severe source condition can reach 10.0–20.0%. From the viewpoint of economy, the relative investment of CASAHP is reduced to 30–60% with a CR of 2.0–3.0. With a 2–6% sacrifice in ESR, the payback period can be reduced from 12–32 to 5–9 years using compression. Partial-design of the CASAHP can further reduce the payback period to 3–6 years with a partial-design ratio of 50% and a CR of 2.8. Additionally, CRs and partial-design ratios are designed comprehensively by seeking the maximum ESR for a given acceptable payback period

  12. Sources of particulate matter components in the Athabasca oil sands region: investigation through a comparison of trace element measurement methodologies

    Directory of Open Access Journals (Sweden)

    C. Phillips-Smith

    2017-08-01

    Full Text Available The province of Alberta, Canada, is home to three oil sands regions which, combined, contain the third largest deposit of oil in the world. Of these, the Athabasca oil sands region is the largest. As part of Environment and Climate Change Canada's program in support of the Joint Canada-Alberta Implementation Plan for Oil Sands Monitoring program, concentrations of trace elements in PM2. 5 (particulate matter smaller than 2.5 µm in diameter were measured through two campaigns that involved different methodologies: a long-term filter campaign and a short-term intensive campaign. In the long-term campaign, 24 h filter samples were collected once every 6 days over a 2-year period (December 2010–November 2012 at three air monitoring stations in the regional municipality of Wood Buffalo. For the intensive campaign (August 2013, hourly measurements were made with an online instrument at one air monitoring station; daily filter samples were also collected. The hourly and 24 h filter data were analyzed individually using positive matrix factorization. Seven emission sources of PM2. 5 trace elements were thereby identified: two types of upgrader emissions, soil, haul road dust, biomass burning, and two sources of mixed origin. The upgrader emissions, soil, and haul road dust sources were identified through both the methodologies and both methodologies identified a mixed source, but these exhibited more differences than similarities. The second upgrader emissions and biomass burning sources were only resolved by the hourly and filter methodologies, respectively. The similarity of the receptor modeling results from the two methodologies provided reassurance as to the identity of the sources. Overall, much of the PM2. 5-related trace elements were found to be anthropogenic, or at least to be aerosolized through anthropogenic activities. These emissions may in part explain the previously reported higher levels of trace elements in snow

  13. Annual investigation of vertical type ground source heat pump system performance on a wall heating and cooling system in Istanbul

    Energy Technology Data Exchange (ETDEWEB)

    Akbulut, U.; Yoru, Y.; Kincay, O. [Department of Mechanical Engineering, Yildiz Technical University (Turkey)], email: akbulutugur@yahoo.com, email: yilmazyoru@gmail.com, email: okincay@yildiz.edu.tr

    2011-07-01

    Wall heating and cooling systems (WHCS) are equipped with heating serpentines or panels for water circulation. These systems operate in a low temperature range so they are preferable to other, conventional systems. Furthermore, when these systems are connected to a ground source heat pump (GSHP) system, energy performance and thermal comfort are further enhanced. The purpose of this paper is to report the results of an annual inspection done on a vertical type ground-coupled heat pump systems (V-GSHP) WHCS in Istanbul and present the results. The performance data from the Yildiz Renewable Energy House at Davutpasa Campus of Yildiz Technical University, Istanbul, Turkey, during the year 2010 were collected and analyzed. The conclusions drawn from the inspection and analysis were listed in this paper. Using renewable energy sources effectively will bring both economic and environmental benefits and it is hoped that the use of these energy efficient WHCS systems will become widespread.

  14. Investigation of a {sup 90}Sr/{sup 90}Y source for intra-ocular treatment of wet age-related macular degeneration

    Energy Technology Data Exchange (ETDEWEB)

    Holmes, Shannon M.; Micka, John A.; DeWerd, Larry A. [University of Wisconsin Medical Radiation Research Center, Madison, Wisconsin 53705 (United States)

    2009-10-15

    Purpose: The purpose of this study is to perform an extensive investigation of an approximately 2.5 mm long {sup 90}Sr/{sup 90}Y source designed for treating wet age-related macular degeneration. Methods: As part of this investigation, a NIST-traceable absorbed dose to water calibration technique was established, and a source deployment verification test was developed. The influence of treatment cannula construction tolerance on the measurements as well as the dose delivered to the patient was investigated using the Monte Carlo code MCNP5. Variation between production cannulae was quantified experimentally using a well-type ionization chamber, and additional measurements along with Monte Carlo calculations of the collimating insert used for source deployment verification were performed to validate the model. Results: Maximum variation in the integrated target dose was seen when the source was shifted laterally within the treatment cannula. For the well chamber measurements, the observed standard deviation in ionization current for a single source placed in different reference cannulae was {+-}0.3%, with a maximum observed range of less than {+-}0.5%. Clinical cannulae in the collimating insert showed an average of 17.8%{+-}0.4% of the reference signal when sources were fully deployed compared to 18.5% predicted by Monte Carlo calculations. This discrepancy has been attributed primarily to construction of the collimator since the collimation gap was observed to be approximately 0.025-0.075 mm smaller than specified. Construction tolerance of the well chamber insert as well as position tolerance of the cannula tip were both investigated, and their influence on the predicted signal was quantified. Additional measurements along with Monte Carlo based calculations of the collimating insert with polyethylene spacers added to the setup were performed to validate the Monte Carlo model. The shimmed Monte Carlo and measured data agree to within 1%, which is a magnitude

  15. Investigation of model based beamforming and Bayesian inversion signal processing methods for seismic localization of underground sources

    DEFF Research Database (Denmark)

    Oh, Geok Lian; Brunskog, Jonas

    2014-01-01

    Techniques have been studied for the localization of an underground source with seismic interrogation signals. Much of the work has involved defining either a P-wave acoustic model or a dispersive surface wave model to the received signal and applying the time-delay processing technique and frequ...... that for field data, inversion for localization is most advantageous when the forward model completely describe all the elastic wave components as is the case of the FDTD 3D elastic model....

  16. Mangroves act as a small methane source: an investigation on 5 pathways of methane emissions from mangroves

    Science.gov (United States)

    Chen, H.; Peng, C.; Guan, W.; Liao, B.; Hu, J.

    2017-12-01

    The methane (CH4) source strength of mangroves is not well understood, especially for integrating all CH4 pathways. This study measured CH4 fluxes by five pathways (sediments, pneumatophores, water surface, leaves, and stems) from four typical mangrove forests in Changning River of Hainan Island, China, including Kandelia candel , Sonneratia apetala, Laguncularia racemosa and Bruguiera gymnoihiza-Bruguiera sexangula. The CH4 fluxes (mean ± SE) from sediments were 4.82 ± 1.46 mg CH4 m-2 h-1 for those without pneumatophores and 1.36 ± 0.17 mg CH4 m-2 h-1 for those with pneumatophores. Among the three communities with pneumatophores, S. apetala community had significantly greater emission rate than the other two. Pneumatophores in S. apetala were found to significantly decrease CH4 emission from sediments (P duck farming. Leaves of mangroves except for K. candel were a weak CH4 sink while stems a weak source. As a whole the 72 ha of mangroves in the Changning river basin emitted about 8.10 Gg CH4 yr-1 with a weighted emission rate of about 1.29 mg CH4 m-2 h-1, therefore only a small methane source to the atmosphere compared to other reported ones. Keywords: Greenhouse Gases; Biogeochemistry; Tropical ecosystems; Methane budget

  17. Supporting the Cybercrime Investigation Process: Effective Discrimination of Source Code Authors Based on Byte-Level Information

    Science.gov (United States)

    Frantzeskou, Georgia; Stamatatos, Efstathios; Gritzalis, Stefanos

    Source code authorship analysis is the particular field that attempts to identify the author of a computer program by treating each program as a linguistically analyzable entity. This is usually based on other undisputed program samples from the same author. There are several cases where the application of such a method could be of a major benefit, such as tracing the source of code left in the system after a cyber attack, authorship disputes, proof of authorship in court, etc. In this paper, we present our approach which is based on byte-level n-gram profiles and is an extension of a method that has been successfully applied to natural language text authorship attribution. We propose a simplified profile and a new similarity measure which is less complicated than the algorithm followed in text authorship attribution and it seems more suitable for source code identification since is better able to deal with very small training sets. Experiments were performed on two different data sets, one with programs written in C++ and the second with programs written in Java. Unlike the traditional language-dependent metrics used by previous studies, our approach can be applied to any programming language with no additional cost. The presented accuracy rates are much better than the best reported results for the same data sets.

  18. Molecular diagnosis of bacterial vaginosis: Does adjustment for total bacterial load or human cellular content improve diagnostic performance?

    Science.gov (United States)

    Plummer, E L; Garland, S M; Bradshaw, C S; Law, M G; Vodstrcil, L A; Hocking, J S; Fairley, C K; Tabrizi, S N

    2017-02-01

    We investigated the utility of quantitative PCR assays for diagnosis of bacterial vaginosis and found that while the best model utilized bacterial copy number adjusted for total bacterial load (sensitivity=98%, specificity=93%, AUC=0.95[95%CI=0.93,0.97]), adjusting for total bacterial or human cell load did not consistently increase the diagnostic performance of the assays. Copyright © 2017 Elsevier B.V. All rights reserved.

  19. Building materials as intrinsic sources of sulphate: A hidden face of salt weathering of historical monuments investigated through multi-isotope tracing (B, O, S)

    International Nuclear Information System (INIS)

    Kloppmann, W.; Bromblet, P.; Vallet, J.M.; Verges-Belmin, V.; Rolland, O.; Guerrot, C.; Gosselin, C.

    2011-01-01

    Sulphate neoformation is a major factor of degradation of stone monuments. Boron, sulphur and oxygen isotope signatures were investigated for five French historical monuments (Bourges, Chartres and Marseille cathedrals, Chenonceau castle, and Versailles garden statues) to investigate the role of intrinsic sulphate sources (gypsum plasters and mortars) in stone degradation, compared to the influence of extrinsic sources such as atmospheric pollution. Gypsum plasters and gypsum-containing mortars fall systematically in the δ 34 S and δ 18 O range of Paris Basin Eocene evaporites indicating the origin of the raw materials (so-called 'Paris plaster'). Black crusts show the typical S and O isotope signatures observed elsewhere in Europe that can be attributed to atmospheric pollution, together with a marine component for Marseille. Boron isotopes for black crusts indicate coal combustion as principal boron source. Mortar isotope compositions discriminate three types, one similar to gypsum plasters, one strongly depleted in 34 S, attributed to pyrite oxidation, and a third one close to atmospheric sulphates. The isotopic composition of sulphates and boron of most degraded building stones of the different monuments is well explained by the identified sulphate sources. In several cases (in particular for Chenonceau and Bourges, to some extent for Chartres), the impact of gypsum plaster as building and restoration material on the degradation of the stones in its vicinity was clearly demonstrated. The study illustrates the usefulness of multi-isotope studies to investigate stone degradation factors, as the combination of several isotope systematics increases the discriminatory power of isotope studies with respect to contaminant sources. - Research Highlights: → Insight in stone weathering mechanisms by multi-isotope fingerprinting (B, S, O). → Intrinsic sulphate sources (gypsum plaster, mortar) contribute to stone degradation. → Origin of building materials

  20. Building materials as intrinsic sources of sulphate: A hidden face of salt weathering of historical monuments investigated through multi-isotope tracing (B, O, S)

    Energy Technology Data Exchange (ETDEWEB)

    Kloppmann, W., E-mail: w.kloppmann@brgm.fr [BRGM, BP 6009, F-45060 Orleans cedex 2 (France); Bromblet, P.; Vallet, J.M. [CICRP, 21, rue Guibal, F-13003 Marseille (France); Verges-Belmin, V. [LRMH, 29, rue de Paris, F-77420 Champs sur Marne (France); Rolland, O. [Independent restorer, 3, rue du Gue, 37270 Montlouis s/Loire (France); Guerrot, C. [BRGM, BP 6009, F-45060 Orleans cedex 2 (France); Gosselin, C. [BRGM, BP 6009, F-45060 Orleans cedex 2 (France); LRMH, 29, rue de Paris, F-77420 Champs sur Marne (France)

    2011-04-01

    Sulphate neoformation is a major factor of degradation of stone monuments. Boron, sulphur and oxygen isotope signatures were investigated for five French historical monuments (Bourges, Chartres and Marseille cathedrals, Chenonceau castle, and Versailles garden statues) to investigate the role of intrinsic sulphate sources (gypsum plasters and mortars) in stone degradation, compared to the influence of extrinsic sources such as atmospheric pollution. Gypsum plasters and gypsum-containing mortars fall systematically in the {delta}{sup 34}S and {delta}{sup 18}O range of Paris Basin Eocene evaporites indicating the origin of the raw materials (so-called 'Paris plaster'). Black crusts show the typical S and O isotope signatures observed elsewhere in Europe that can be attributed to atmospheric pollution, together with a marine component for Marseille. Boron isotopes for black crusts indicate coal combustion as principal boron source. Mortar isotope compositions discriminate three types, one similar to gypsum plasters, one strongly depleted in {sup 34}S, attributed to pyrite oxidation, and a third one close to atmospheric sulphates. The isotopic composition of sulphates and boron of most degraded building stones of the different monuments is well explained by the identified sulphate sources. In several cases (in particular for Chenonceau and Bourges, to some extent for Chartres), the impact of gypsum plaster as building and restoration material on the degradation of the stones in its vicinity was clearly demonstrated. The study illustrates the usefulness of multi-isotope studies to investigate stone degradation factors, as the combination of several isotope systematics increases the discriminatory power of isotope studies with respect to contaminant sources. - Research Highlights: {yields} Insight in stone weathering mechanisms by multi-isotope fingerprinting (B, S, O). {yields} Intrinsic sulphate sources (gypsum plaster, mortar) contribute to stone degradation

  1. Bacterial lung abscess

    International Nuclear Information System (INIS)

    Groskin, S.A.; Panicek, D.M.; Ewing, D.K.; Rivera, F.; Math, K.; Teixeira, J.; Heitzman, E.R.

    1987-01-01

    A retrospective review of patients with bacterial lung abscess was carried out. Demographic, clinical, and radiographical features of this patient group are compared with similar data from patients with empyema and/or cavitated lung carcinoma; differential diagnostic points are stressed. The entity of radiographically occult lung abscess is discussed. Complications associated with bacterial lung abscess are discussed. Current therapeutic options and treatment philosophy for patients with bacterial lung abscess are noted

  2. Peritonitis - spontaneous bacterial

    Science.gov (United States)

    Spontaneous bacterial peritonitis (SBP); Ascites - peritonitis; Cirrhosis - peritonitis ... who are on peritoneal dialysis for kidney failure. Peritonitis may have other causes . These include infection from ...

  3. Investigation on potential landslide sources along the Hyblaean-Malta escarpment for the 1693 tsunami in Eastern Sicily (Southern Italy)

    Science.gov (United States)

    Zaniboni, Filippo; Pagnoni, Gianluca; Armigliato, Alberto; Tinti, Stefano

    2015-04-01

    The study of the source of 1693 tsunami in eastern Sicily (South Italy) is still debated in the scientific community. Macroseismic analyses provide inland location for the epicenter of the earthquake, while historical reports describing 1-2 m waves hitting the coast suggest the existence of at least an offshore extension of the fault. Furthermore, an anomalous water elevation was described in Augusta (between Siracusa and Catania), that was interpreted as the manifestation of a local submarine landslide. The presence of the steep Hyblaean-Malta escarpment, that runs parallel to the eastern coast of Sicily at a short distance from the shoreline and is cut by several canyons and scars, corroborates the hypothesis of a landslide occurrence, though no clear evidence has been found yet. This research, realized in the frame of the project ASTARTE (Assessment, Strategy And Risk Reduction for Tsunamis in Europe - FP7-ENV2013 6.4-3, Grant 603839), aims at assessing the effect of landslide-generated tsunamis on the coastal stretch around Augusta considering different scenarios of collapsing masses along the Hyblaean-Malta escarpment. The slide dynamics is computed by means of the numerical code UBO-BLOCK1 (developed by the University of Bologna Tsunami Research Team), and the corresponding tsunami is simulated via the code UBO-TSUFD. The sliding bodies are placed in different positions in order to assess which of them could produce significant effects on the town of Augusta, providing then clues on the possible source area for the hypothesized slide related to the 1693 tsunami. The sensitivity analysis shows the spatial dependence of the coastal tsunami height on the source volume, position, distance from the coast, and on other parameters.

  4. California GAMA Special Study: An isotopic and dissolved gas investigation of nitrate source and transport to a public supply well in California's Central Valley

    Energy Technology Data Exchange (ETDEWEB)

    Singleton, M J; Moran, J E; Esser, B K; Roberts, S K; Hillegonds, D J

    2010-04-14

    This study investigates nitrate contamination of a deep municipal drinking water production well in Ripon, CA to demonstrate the utility of natural groundwater tracers in constraining the sources and transport of nitrate to deep aquifers in the Central Valley. The goal of the study was to investigate the origin (source) of elevated nitrate and the potential for the deep aquifer to attenuate anthropogenic nitrate. The site is ideal for such an investigation. The production well is screened from 165-325 feet below ground surface and a number of nearby shallow and deep monitoring wells were available for sampling. Furthermore, potential sources of nitrate contamination to the well had been identified, including a fertilizer supply plant located approximately 1000 feet to the east and local almond groves. A variety of natural isotopic and dissolved gas tracers including {sup 3}H-{sup 3}He groundwater age and the isotopic composition of nitrate are applied to identify nitrate sources and to characterize nitrate transport. An advanced method for sampling production wells is employed to help identify contaminant contributions from specific screen intervals. Nitrate transport: Groundwater nitrate at this field site is not being actively denitrified. Groundwater parameters indicate oxic conditions, the dissolved gas data shows no evidence for excess nitrogen as the result of denitrification, and nitrate-N and -O isotope compositions do not display patterns typical of denitrification. Contaminant nitrate source: The ambient nitrate concentration in shallow groundwater at the Ripon site ({approx}12 mg/L as nitrate) is typical of shallow groundwaters affected by recharge from agricultural and urban areas. Nitrate concentrations in Ripon City Well 12 (50-58 mg/L as nitrate) are significantly higher than these ambient concentrations, indicating an additional source of anthropogenic nitrate is affecting groundwater in the capture zone of this municipal drinking water well. This

  5. Investigation of gasoline distributions within petrol stations: spatial and seasonal concentrations, sources, mitigation measures, and occupationally exposed symptoms.

    Science.gov (United States)

    Sairat, Theerapong; Homwuttiwong, Sahalaph; Homwutthiwong, Kritsana; Ongwandee, Maneerat

    2015-09-01

    We measured levels of VOCs and determined the distributions of benzene concentrations over the area of two petrol stations in all three seasons. Using the concentrations and sampling positions, we created isoconcentration contour maps. The average concentrations ranged 18-1288 μg m(-3) for benzene and 12-81 μg m(-3) for toluene. The contour maps indicate that high-level contours of benzene were found not only at the fuel dispenser areas but also at the storage tank refilling points, open drainage areas where gasoline-polluted wastewater was flowing, and the auto service center located within the station area. An assessment of the benzene to toluene ratio contour plots implicates that airborne benzene and toluene near the fuel dispenser area were attributed to gasoline evaporation although one of the studied stations may be influenced by other VOC sources besides gasoline evaporation. Additionally, during the routine refilling of the underground fuel storage tanks by a tank truck, the ambient levels of benzene and toluene increased tremendously. The implementation of source control by replacing old dispensers with new fuel dispensers that have an efficient cutoff feature and increased delivery speed can reduce spatial benzene concentrations by 77%. Furthermore, a questionnaire survey among 63 service attendants in ten stations revealed that headache was the most reported health complaint with a response rate of 32%, followed by fatigue with 20%. These prominent symptoms could be related to an exposure to high benzene concentrations.

  6. Investigation of waste incineration of fluorotelomer-based polymers as a potential source of PFOA in the environment.

    Science.gov (United States)

    Taylor, P H; Yamada, T; Striebich, R C; Graham, J L; Giraud, R J

    2014-09-01

    In light of the widespread presence of perfluorooctanoic acid (PFOA) in the environment, a comprehensive laboratory-scale study has developed data requested by the U.S. Environmental Protection Agency (EPA) to determine whether municipal and/or medical waste incineration of commercial fluorotelomer-based polymers (FTBPs) at end of life is a potential source of PFOA that may contribute to environmental and human exposures. The study was divided into two phases (I and II) and conducted in accordance with EPA Good Laboratory Practices (GLPs) as described in the quality assurance project plan (QAPP) for each phase. Phase I testing determined that the PFOA transport efficiency across the thermal reactor system to be used in Phase II was greater than 90%. Operating at 1000°C over 2s residence time with 3.2-6.6mgdscm(-1) hydrogen fluoride (HF), corrected to 7% oxygen (O2), and continuously monitored exhaust oxygen of 13%, Phase II testing of the FTBP composites in this thermal reactor system yielded results demonstrating that waste incineration of fluorotelomer-based polymers does not result in the formation of detectable levels of PFOA under conditions representative of typical municipal waste combustor (MWC) and medical waste incinerator (MWI) operations in the U.S. Therefore, waste incineration of these polymers is not expected to be a source of PFOA in the environment. Copyright © 2014 Elsevier Ltd. All rights reserved.

  7. Investigating the error sources of the online state of charge estimation methods for lithium-ion batteries in electric vehicles

    Science.gov (United States)

    Zheng, Yuejiu; Ouyang, Minggao; Han, Xuebing; Lu, Languang; Li, Jianqiu

    2018-02-01

    Sate of charge (SOC) estimation is generally acknowledged as one of the most important functions in battery management system for lithium-ion batteries in new energy vehicles. Though every effort is made for various online SOC estimation methods to reliably increase the estimation accuracy as much as possible within the limited on-chip resources, little literature discusses the error sources for those SOC estimation methods. This paper firstly reviews the commonly studied SOC estimation methods from a conventional classification. A novel perspective focusing on the error analysis of the SOC estimation methods is proposed. SOC estimation methods are analyzed from the views of the measured values, models, algorithms and state parameters. Subsequently, the error flow charts are proposed to analyze the error sources from the signal measurement to the models and algorithms for the widely used online SOC estimation methods in new energy vehicles. Finally, with the consideration of the working conditions, choosing more reliable and applicable SOC estimation methods is discussed, and the future development of the promising online SOC estimation methods is suggested.

  8. Prevalence of antibiotic resistance genes in bacterial communities associated with Cladophora glomerata mats along the nearshore of Lake Ontario.

    Science.gov (United States)

    Ibsen, Michael; Fernando, Dinesh M; Kumar, Ayush; Kirkwood, Andrea E

    2017-05-01

    The alga Cladophora glomerata can erupt in nuisance blooms throughout the lower Great Lakes. Since bacterial abundance increases with the emergence and decay of Cladophora, we investigated the prevalence of antibiotic resistance (ABR) in Cladophora-associated bacterial communities up-gradient and down-gradient from a large sewage treatment plant (STP) on Lake Ontario. Although STPs are well-known sources of ABR, we also expected detectable ABR from up-gradient wetland communities, since they receive surface run-off from urban and agricultural sources. Statistically significant differences in aquatic bacterial abundance and ABR were found between down-gradient beach samples and up-gradient coastal wetland samples (ANOVA, Holm-Sidak test, p Cladophora sampled near the STP had the highest bacterial densities overall, including on ampicillin- and vancomycin-treated plates. However, quantitative polymerase chain reaction analysis of the ABR genes ampC, tetA, tetB, and vanA from environmental communities showed a different pattern. Some of the highest ABR gene levels occurred at the 2 coastal wetland sites (vanA). Overall, bacterial ABR profiles from environmental samples were distinguishable between living and decaying Cladophora, inferring that Cladophora may control bacterial ABR depending on its life-cycle stage. Our results also show how spatially and temporally dynamic ABR is in nearshore aquatic bacteria, which warrants further research.

  9. Combining stable isotopes with contamination indicators: A method for improved investigation of nitrate sources and dynamics in aquifers with mixed nitrogen inputs.

    Science.gov (United States)

    Minet, E P; Goodhue, R; Meier-Augenstein, W; Kalin, R M; Fenton, O; Richards, K G; Coxon, C E

    2017-11-01

    Excessive nitrate (NO 3 - ) concentration in groundwater raises health and environmental issues that must be addressed by all European Union (EU) member states under the Nitrates Directive and the Water Framework Directive. The identification of NO 3 - sources is critical to efficiently control or reverse NO 3 - contamination that affects many aquifers. In that respect, the use of stable isotope ratios 15 N/ 14 N and 18 O/ 16 O in NO 3 - (expressed as δ 15 N-NO 3 - and δ 18 O-NO 3 - , respectively) has long shown its value. However, limitations exist in complex environments where multiple nitrogen (N) sources coexist. This two-year study explores a method for improved NO 3 - source investigation in a shallow unconfined aquifer with mixed N inputs and a long established NO 3 - problem. In this tillage-dominated area of free-draining soil and subsoil, suspected NO 3 - sources were diffuse applications of artificial fertiliser and organic point sources (septic tanks and farmyards). Bearing in mind that artificial diffuse sources were ubiquitous, groundwater samples were first classified according to a combination of two indicators relevant of point source contamination: presence/absence of organic point sources (i.e. septic tank and/or farmyard) near sampling wells and exceedance/non-exceedance of a contamination threshold value for sodium (Na + ) in groundwater. This classification identified three contamination groups: agricultural diffuse source but no point source (D+P-), agricultural diffuse and point source (D+P+) and agricultural diffuse but point source occurrence ambiguous (D+P±). Thereafter δ 15 N-NO 3 - and δ 18 O-NO 3 - data were superimposed on the classification. As δ 15 N-NO 3 - was plotted against δ 18 O-NO 3 - , comparisons were made between the different contamination groups. Overall, both δ variables were significantly and positively correlated (p contamination groups revealed that denitrification did not occur in the absence of point

  10. Use of the HadGEM2 climate-chemistry model to investigate interannual variability in methane sources

    Science.gov (United States)

    Hayman, Garry; O'Connor, Fiona; Clark, Douglas; Huntingford, Chris; Gedney, Nicola

    2013-04-01

    The global mean atmospheric concentration of methane (CH4) has more than doubled during the industrial era [1] and now constitutes ? 20% of the anthropogenic climate forcing by greenhouse gases [2]. The globally-averaged CH4 growth rate, derived from surface measurements, has fallen significantly from a high of 16 ppb yr-1 in the late 1970s/early 1980s and was close to zero between 1999 and 2006 [1]. This overall period of declining or low growth was however interspersed with years of positive growth-rate anomalies (e.g., in 1991-1992, 1998-1999 and 2002-2003). Since 2007, renewed growth has been evident [1, 3], with the largest increases observed over polar northern latitudes and the Southern Hemisphere in 2007 and in the tropics in 2008. The observed inter-annual variability in atmospheric methane concentrations and the associated changes in growth rates have variously been attributed to changes in different methane sources and sinks [1, 4]. In this paper, we report results from runs of the HadGEM2 climate-chemistry model [5] using year- and month-specific emission datasets. The HadGEM2 model includes the comprehensive atmospheric chemistry and aerosol package, the UK Chemistry Aerosol community model (UKCA, http://www.ukca.ac.uk/wiki/index.php). The Standard Tropospheric Chemistry scheme was selected for this work. This chemistry scheme simulates the Ox, HOx and NOx chemical cycles and the oxidation of CO, methane, ethane and propane. Year- and month-specific emission datasets were generated for the period from 1997 to 2009 for the emitted species in the chemistry scheme (CH4, CO, NOx, HCHO, C2H6, C3H8, CH3CHO, CH3CHOCH3). The approach adopted varied depending on the source sector: Anthropogenic: The emissions from anthropogenic sources were based on decadal-averaged emission inventories compiled by [6] for the Coupled Carbon Cycle Climate Model Intercomparison Project (C4MIP). These were then used to derive year-specific emission datasets by scaling the

  11. Laser source for dimensional metrology: investigation of an iodine stabilized system based on narrow linewidth 633 nm DBR diode

    Science.gov (United States)

    Rerucha, Simon; Yacoot, Andrew; Pham, Tuan M.; Cizek, Martin; Hucl, Vaclav; Lazar, Josef; Cip, Ondrej

    2017-04-01

    We demonstrated that an iodine stabilized distributed Bragg reflector (DBR) diode based laser system lasing at a wavelength in close proximity to λ =633 nm could be used as an alternative laser source to the helium-neon lasers in both scientific and industrial metrology. This yields additional advantages besides the optical frequency stability and coherence: inherent traceability, wider optical frequency tuning range, higher output power and high frequency modulation capability. We experimentally investigated the characteristics of the laser source in two major steps: first using a wavelength meter referenced to a frequency comb controlled with a hydrogen maser and then on an interferometric optical bench testbed where we compared the performance of the laser system with that of a traditional frequency stabilized He-Ne laser. The results indicate that DBR diode laser system provides a good laser source for applications in dimensional (nano)metrology, especially in conjunction with novel interferometric detection methods exploiting high frequency modulation or multiaxis measurement systems.

  12. Investigation of some possible changes in Am-Be neutron source configuration in order to increase the thermal neutron flux using Monte Carlo code

    Science.gov (United States)

    Basiri, H.; Tavakoli-Anbaran, H.

    2018-01-01

    Am-Be neutrons source is based on (α, n) reaction and generates neutrons in the energy range of 0-11 MeV. Since the thermal neutrons are widely used in different fields, in this work, we investigate how to improve the source configuration in order to increase the thermal flux. These suggested changes include a spherical moderator instead of common cylindrical geometry, a reflector layer and an appropriate materials selection in order to achieve the maximum thermal flux. All calculations were done by using MCNP1 Monte Carlo code. Our final results indicated that a spherical paraffin moderator, a layer of beryllium as a reflector can efficiently increase the thermal neutron flux of Am-Be source.

  13. Chapter 27: Deja vu All Over Again: Using NVO Tools to Re-Investigate a Complete Sample of Texas Radio Survey Sources

    Science.gov (United States)

    Lucas, Ray A.; Rohde, David; Tamura, Takayuki; van Dyne, Jeffrey

    At the first NVO Summer School in September 2004, a complete sample of Texas Radio Survey sources, first derived in 1989 and subsequently observed with the VLA in A-array snapshot mode in 1990, was revisited. The original investigators had never had the occasion to reduce the A-array 5-minute snapshot data, nor to do any other significant follow-up, though the sample still seemed a possibly useful but relatively small study of radio galaxies, AGN, quasars, extragalactic sources, and galaxy clusters, etc. At the time of the original sample definition in late 1989, the best optical material available for the region was the SRC-J plate from the UK Schmidt Telescope in Australia. In much more recent times, the Sloan Digital Sky Survey has included the region in its DR2 data release, so good multicolor optical imaging in a number of standard bandpasses has finally become available. These data, along with other material in the radio, infrared, and (where available) were used to get a better preliminary idea of the nature of the objects in the 1989 sample. We also investigated one of the original questions: whether these radio sources with steeper (or at least non-flat) radio spectra were associated with galaxy clusters, and in some cases higher-redshift galaxy clusters and AGN. A rudimentary web service was created which allowed the user to perform simple cone searches and SIAP image extractions of specified field sizes for multiwavelength data across the electromagnetic spectrum, and a prototype web page was set up which would display the resulting images in wavelength order across the page for sources in the sample. Finally, as an additional investigation, using radio and X-ray IDs as a proxy for AGN which might be associated with large, central cluster galaxies, positional matches of radio and X-ray sources from two much larger catalogs were done using the tool TOPCAT in order to search for the degree of correlation between ID positions, radio luminosity, and cluster

  14. Investigation of the Makeup, Source, and Removal Strategies for Total Organic Carbon in the Oxygen Generation System Recirculation Loop

    Science.gov (United States)

    Bowman, Elizabeth M.; Carpenter, Joyce; Roy, Robert J.; Van Keuren, Steve; Wilson, Mark E.

    2015-01-01

    Since 2007, the Oxygen Generation System (OGS) on board the International Space Station (ISS) has been producing oxygen for crew respiration via water electrolysis. As water is consumed in the OGS recirculating water loop, make-up water is furnished by the ISS potable water bus. A rise in Total Organic Carbon (TOC) was observed beginning in February, 2011, which continues through the present date. Increasing TOC is of concern because the organic constituents responsible for the TOC were unknown and had not been identified; hence their impacts on the operation of the electrolytic cell stack components and on microorganism growth rates and types are unknown. Identification of the compounds responsible for the TOC increase, their sources, and estimates of their loadings in the OGA as well as possible mitigation strategies are presented.

  15. Investigations of the inductively coupled plasma source for analyzing NURE water samples at the Los Alamos Scientific Laboratory

    International Nuclear Information System (INIS)

    Apel, C.T.; Bieniewski, T.M.; Cox, L.E.; Steinhaus, D.W.

    1977-03-01

    A 3.4-meter direct-reading spectrograph is being used with an inductively coupled plasma source for the simultaneous determination of Ag, Bi, Cd, Cu, Nb, Ni, Pb, Sn, and W in water samples. We have attached a small digital computer to the system in order to obtain intensity data on each element once a second. After the intensities during a run on a sample have stabilized, the computer records the intensity data and outputs the average concentration for each element. To approach the published detection limits, a peristaltic pump must be used to force the water sample into the usual cross-flow nebulizer. We have studied several different nebulizer designs with the goal of improving efficiency and hence sensitivity. One design, the fritted-disk nebulizer, has an efficiency over 60 percent, as compared with the 5 percent efficiency of the original nebulizer

  16. Nebulizadores: fonte de contaminação bacteriana em pacientes com fibrose cística? Nebulizers in cystic fibrosis: a source of bacterial contamination in cystic fibrosis patients?

    Directory of Open Access Journals (Sweden)

    Lorena Xavier Costa Brzezinski

    2011-06-01

    were not previously informed of the purpose of the visit. Three samples were collected from each patient: one from the nebulizer chamber, one from the mask/mouthpiece, and one from the patient (oropharyngeal swab /sputum. The samples were properly stored and taken for analyses. The patients, their parents, or their legal guardians completed a questionnaire regarding nebulizer cleaning and disinfecting methods. RESULTS: We collected 84 samples from the 28 patients. Of those 28 patients, 15 (53.5% were male. The median age of the patients was 11 years (range, 1-27 years. Of the 28 patients, 15 presented with positive oropharyngeal swab /sputum sample cultures. The most common bacterial isolates were Staphylococcus aureus (in 8 patients and Pseudomonas aeruginosa (in 4 patients. Although the samples obtained from the nebulizers presented with various pathogens in culture, no specific species predominated. In 27 cases (96.7%, there were no associations between the samples obtained from the nebulizers and those obtained from the patients in terms of the results of the cultures. Cleaning and disinfection of nebulizers were inappropriate in 22 cases (78.6%. CONCLUSIONS: In this sample of patients, despite the inappropriate disinfection techniques, nebulizers were not found to be a source of microbial contamination

  17. Cost-effectiveness modeling for neuropathic pain treatments: investigating the relative importance of parameters using an open-source model.

    Science.gov (United States)

    Hirst, Matthew; Bending, Matthew W; Baio, Gianluca; Yesufu-Udechuku, Amina; Dunlop, William C N

    2018-06-08

    The study objective was to develop an open-source replicate of a cost-effectiveness model developed by National Institute for Health and Care (NICE) in order to explore uncertainties in health economic modeling of novel pharmacological neuropathic pain treatments. The NICE model, consisting of a decision tree with branches for discrete levels of pain relief and adverse event (AE) severities, was replicated using R and used to compare a hypothetical neuropathic pain drug to pregabalin. Model parameters were sourced from NICE's clinical guidelines and associated with probability distributions to account for underlying uncertainty. A simulation-based scenario analysis was conducted to assess how uncertainty in efficacy and AEs affected the net monetary benefit (NMB) for the hypothetical treatment at a cost-effectiveness threshold of £20,000 per QALY. Relative to pregabalin, an increase in efficacy was associated with greater NMB than an improvement in tolerability. A greater NMB was observed when efficacy was marginally higher than that of pregabalin while maintaining the same level of AEs than when efficacy was equivalent to pregabalin but with a more substantial reduction in AEs. In the latter scenario, the NMB was only positive at a low cost-effectiveness threshold. The replicate model shares the limitations described in the NICE guidelines. There is a lack of support in scientific literature for the assumption that increased efficacy is associated with a greater reduction in tolerability. The replicate model also included a single comparator, unlike the NICE model. Pain relief is a stronger driver of NMB than tolerability at a cost-effectiveness threshold of £20,000 per QALY. Health technology assessment decisions which are influenced by NICE's model may reward efficacy gains even if they are associated with more severe AEs. This contrasts with recommendations from clinical guidelines for neuropathic pain which place more equal weighting on improvements in

  18. Investigation of the relationships between DCS cloud properties, lifecycle, and precipitation with meteorological regimes and aerosol sources at the ARM SGP Site

    Energy Technology Data Exchange (ETDEWEB)

    Dong, Xiquan [Univ. of North Dakota, Grand Forks, ND (United States). Dept. of Atmospheric Sciences; Univ. of Arizona, Tucson, AZ (United States). Dept. of Hydrology and Atmospheric Sciences

    2016-10-26

    In this proposed research, we will investigate how different meteorological regimes and aerosol sources affect DCS properties, diurnal and life cycles, and precipitation using multiple observational platforms (surface, satellite, and aircraft) and NARR reanalysis at the ARM SGP site. The Feng et al. (2011, 2012) DCS results will serve as a starting point for this proposed research, and help us to address some fundamental issues of DCSs, such as convective initiation, rain rate, areal extent (including stratiform and convective regions), and longevity. Convective properties will be stratified by meteorological regime (synoptic/mesoscale patterns) identified by reanalysis. Aerosol information obtained from the ARM SGP site will also be stratified by meteorological regimes to understand their effects on convection. Finally, the aircraft in-situ measurements and various radar observations and retrievals during the MC3E campaign will provide a “cloud-truth” dataset and are an invaluable data source for verifying the findings and investigating the proposed hypotheses in Objective 1.

  19. Prevention of bacterial adhesion

    DEFF Research Database (Denmark)

    Klemm, Per; Vejborg, Rebecca Munk; Hancock, Viktoria

    2010-01-01

    . As such, adhesion represents the Achilles heel of crucial pathogenic functions. It follows that interference with adhesion can reduce bacterial virulence. Here, we illustrate this important topic with examples of techniques being developed that can inhibit bacterial adhesion. Some of these will become...

  20. Investigation on the characteristics of liquid wastes depending on their generation sources and study on optimum treatment method

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Sang Guk; Kim, Dong Chan; Shin, Dae Hyun; Son, Seung Geun; Roh, Nam Sun; Woo, Je Kyung [Korea Inst. of Energy Research, Taejon (Korea, Republic of)

    1995-12-01

    The major research contents conducted this year are as follows: (1) environmental regulation with respect to the treatment of the liquid waste in the U.S.A., (2) the present status of the generation and treatment of liquid wastes for large producers(>1,000 ton/year), (3) analysis for heating value element, heavy metal content, halogenated species on collected samples, (4) investigation on estimation method of energy recovery rate from liquid waste, (5) design of a lab. scale reactor which could be capable of conducting thermal decomposition test with small quantity of sample. In this study, present status of liquid waste generation and treatment is investigated, and thermal decomposition characteristics are studied using a lab. scale thermal reactor. The purpose of this research is to divide liquid waste into groups and to present best treatment method for their each group. (author). 24 refs., 21 figs., 23 tabs.

  1. Response of Archaeal and Bacterial Soil Communities to Changes Associated with Outdoor Cattle Overwintering

    OpenAIRE

    Chro??kov?, Alica; Schloter-Hai, Brigitte; Radl, Viviane; Endesfelder, David; Quince, Christopher; Elhottov?, Dana; ?imek, Miloslav; Schloter, Michael

    2015-01-01

    Archaea and bacteria are important drivers for nutrient transformations in soils and catalyse the production and consumption of important greenhouse gases. In this study, we investigate changes in archaeal and bacterial communities of four Czech grassland soils affected by outdoor cattle husbandry. Two show short-term (3 years; STI) and long-term impact (17 years; LTI), one is regenerating from cattle impact (REG) and a control is unaffected by cattle (CON). Cattle manure (CMN), the source of...

  2. Effect of electron beam irradiation on bacterial cellulose membranes used as transdermal drug delivery systems

    Energy Technology Data Exchange (ETDEWEB)

    Stoica-Guzun, Anicuta [Department of Chemical Engineering, ' Politehnica' University Bucharest, 313 Splaiul Independentei, 060042 Bucharest (Romania)], E-mail: astoica@mt.pub.ro; Stroescu, Marta; Tache, Florin [Department of Chemical Engineering, ' Politehnica' University Bucharest, 313 Splaiul Independentei, 060042 Bucharest (Romania); Zaharescu, Traian [Advanced Research Institute for Electrical Engineering, 313 Splaiul Unirii, 030138 Bucharest (Romania)], E-mail: zaharescut@icpe-ca.ro; Grosu, Elena [Department of Chemical Engineering, ' Politehnica' University Bucharest, 313 Splaiul Independentei, 060042 Bucharest (Romania)

    2007-12-15

    Ionizing radiation is an effective energetic source for polymer surfaces modification in order to obtain transdermal systems with different controlled release properties. In this work, gamma rays have been applied to induce changes in bacterial cellulose membranes. Permeation of drug (tetracycline) was theoretically and experimentally investigated starting from the effect of {gamma}-irradiation on membranes permeability. Release and permeation of drug from irradiated and non-irradiated membranes have been performed using a diffusion cell.

  3. Effect of electron beam irradiation on bacterial cellulose membranes used as transdermal drug delivery systems

    International Nuclear Information System (INIS)

    Stoica-Guzun, Anicuta; Stroescu, Marta; Tache, Florin; Zaharescu, Traian; Grosu, Elena

    2007-01-01

    Ionizing radiation is an effective energetic source for polymer surfaces modification in order to obtain transdermal systems with different controlled release properties. In this work, gamma rays have been applied to induce changes in bacterial cellulose membranes. Permeation of drug (tetracycline) was theoretically and experimentally investigated starting from the effect of γ-irradiation on membranes permeability. Release and permeation of drug from irradiated and non-irradiated membranes have been performed using a diffusion cell

  4. Effect of electron beam irradiation on bacterial cellulose membranes used as transdermal drug delivery systems

    Science.gov (United States)

    Stoica-Guzun, Anicuta; Stroescu, Marta; Tache, Florin; Zaharescu, Traian; Grosu, Elena

    2007-12-01

    Ionizing radiation is an effective energetic source for polymer surfaces modification in order to obtain transdermal systems with different controlled release properties. In this work, gamma rays have been applied to induce changes in bacterial cellulose membranes. Permeation of drug (tetracycline) was theoretically and experimentally investigated starting from the effect of γ-irradiation on membranes permeability. Release and permeation of drug from irradiated and non-irradiated membranes have been performed using a diffusion cell.

  5. Bacterial Diversity in Submarine Groundwater along the Coasts of the Yellow Sea

    OpenAIRE

    Ye, Qi; Liu, Jianan; Du, Jinzhou; Zhang, Jing

    2016-01-01

    Submarine groundwater (SGD) is one of the most significant pathways for the exchange of groundwater and/or source of nutrients, metals and carbon to the ocean, subsequently cause deleterious impacts on the coastal ecosystems. Microorganisms have been recognized as the important participators in the biogeochemical processes in the SGD. In this study, by utilizing 16S rRNA-based Illumina Miseq sequencing technology, we investigated bacterial diversity and distribution in both fresh well water a...

  6. Dual character of Sundarban estuary as a source and sink of CO2 during summer: an investigation of spatial dynamics.

    Science.gov (United States)

    Akhand, Anirban; Chanda, Abhra; Dutta, Sachinandan; Manna, Sudip; Sanyal, Pranabes; Hazra, Sugata; Rao, K H; Dadhwal, V K

    2013-08-01

    A comprehensive attempt has been made to evaluate the diurnal and spatial pattern of CO2 exchange between the atmosphere and water along the estuarine track of Indian Sundarbans during the two summer months, April and May, 2011. Rigorous field observations were carried out which included the hourly measurements of total alkalinity, pH, fugacity of CO2 in ambient air and water surface, dissolved oxygen, and chlorophyll a. The estuarine water was found rich in total alkalinity and was oversaturated with CO2 throughout the diurnal cycle in the two stations situated at the inner and middle estuary, respectively, whereas an entirely reverse situation was observed in the outer fringes. The fugacity of CO2 in water ranged from 152 to 657 μatm during the study period. The percentage of over-saturation in inner and middle estuary varied from 103 to 168 and 103 to 176 %, respectively, whereas the degree of under-saturation in the outer estuary lied between 40 and 99 %. Chlorophyll a concentrations were found higher in the outer estuary (12.3 ± 2.2 mg m(-3)) compared to the middle (6.4 ± 0.6 mg m(-3)) and inner parts (1.6 ± 0.2 mg m(-3)), followed by a similar decreasing pattern in nutrient availability from the outer to inner estuary. The sampling stations situated at the inner and middle estuary acted as a net source of 29.69 and 23.62 mg CO2 m(-2) day(-1), respectively, whereas the outer station behaved as a net sink of -33.37 mg CO2 m(-2) day(-1). The study of primary production and community respiration further supports the heterotrophic nature of the estuary in the inner region while the outer periphery was marked by dominant autotrophic character. These contrasting results are in parity with the source characters of many inner estuaries and sinking characters of the outer estuaries situated at the distal continental shelf areas.

  7. Hf and Nd Isotopic and REE Investigations of Magnetite in a Proterozoic IOCG system: Fingerprinting Sources and Timing of Mineralisation

    Science.gov (United States)

    Schaefer, B. F.

    2016-12-01

    The Stuart Shelf on the margin of the Gawler Craton, South Australia, contains numerous economic and sub-econmic IOCG mineralised systems, including the giant Olympic Dam Cu-Au-U deposit. Hematite and magnetite have played a critical in the genesis of all of these deposits, and increasingly it appears that magnetite has been in equilibrium with either the final mineralised assemblage or was critical in transporting metals during the ore forming event. 14 magnetites and one hematite from three separate styles of iron oxide mineralisation associated with the Prominent Hill Cu-Au deposit were selected for detailed analysis. The REE and isotopic separations were all conducted by low blank wet chemistry and isotopes determined by TIMS (Nd) and MC-ICPMS (Hf). Magnetites associated with skarn style mineralsiation proximal to the ore body are unformly depleted in REE, whereas hematite within the ore and magmatic magmatites and whole rock gabbros from the nearby 1590Ma White Hill Gabbro intrusion are all relatively LREE enriched and display a comparable range in REE. Significantly however, magnetite separates almost invariably display more evolved Hf isotopic signatures than the host lithologies adjacent the economic mineralisation (dacites and metasediments at Prominent Hill mine) implying that the magnetites were sourcing their REE inventory dominantly from the local crust rather than a mantle derived source. In contrast, the magmatic magnetites from the White Hill Complex display Nd and Hf isotopes which are slightly more primitive, recording a greater relative mantle component, however still requiring a significant crustal input. Significantly, the hematite which contains the Au mineralisation preserves ɛNd (1590) = -4.04 and ɛHf (1590) = -6.05 essentially identical to the magmatic magnetites and their host gabbros in the White Hill complex and the basalts and dacites of the host Gawler Range Volcanics (ɛNd (1590) = -7.10 - -3.72 and ɛHf (1590) = -7.69 - -1

  8. The Bacterial Sequential Markov Coalescent.

    Science.gov (United States)

    De Maio, Nicola; Wilson, Daniel J

    2017-05-01

    Bacteria can exchange and acquire new genetic material from other organisms directly and via the environment. This process, known as bacterial recombination, has a strong impact on the evolution of bacteria, for example, leading to the spread of antibiotic resistance across clades and species, and to the avoidance of clonal interference. Recombination hinders phylogenetic and transmission inference because it creates patterns of substitutions (homoplasies) inconsistent with the hypothesis of a single evolutionary tree. Bacterial recombination is typically modeled as statistically akin to gene conversion in eukaryotes, i.e. , using the coalescent with gene conversion (CGC). However, this model can be very computationally demanding as it needs to account for the correlations of evolutionary histories of even distant loci. So, with the increasing popularity of whole genome sequencing, the need has emerged for a faster approach to model and simulate bacterial genome evolution. We present a new model that approximates the coalescent with gene conversion: the bacterial sequential Markov coalescent (BSMC). Our approach is based on a similar idea to the sequential Markov coalescent (SMC)-an approximation of the coalescent with crossover recombination. However, bacterial recombination poses hurdles to a sequential Markov approximation, as it leads to strong correlations and linkage disequilibrium across very distant sites in the genome. Our BSMC overcomes these difficulties, and shows a considerable reduction in computational demand compared to the exact CGC, and very similar patterns in simulated data. We implemented our BSMC model within new simulation software FastSimBac. In addition to the decreased computational demand compared to previous bacterial genome evolution simulators, FastSimBac provides more general options for evolutionary scenarios, allowing population structure with migration, speciation, population size changes, and recombination hotspots. FastSimBac is

  9. An Investigation into Spent Coffee Waste as a Renewable Source of Bioactive Compounds and Industrially Important Sugars

    Directory of Open Access Journals (Sweden)

    Damhan S. Scully

    2016-11-01

    Full Text Available Conventional coffee brewing techniques generate vast quantities of spent espresso grounds (SEGs rich in lignocellulose and valuable bioactives. These bioactive compounds can be exploited as a nutraceutical or used in a range of food products, while breakdown of lignocellulose generates metabolizable sugars that can be used for the production of various high-value products such as biofuels, amino acids and enzymes. Response surface methodology (RSM was used to optimize the enzymatic saccharification of lignocellulose in SEGs following a hydrothermal pretreatment. A maximum reducing sugar yield was obtained at the following optimized hydrolysis conditions: 4.97 g of pretreated SEGs, 120 h reaction time, and 1246 and 250 µL of cellulase and hemicellulase, respectively. Industrially important sugars (glucose, galactose and mannose were identified as the principal hydrolysis products under the studied conditions. Total flavonoids (p = 0.0002, total polyphenols (p = 0.03 and DPPH free-radical scavenging activity (p = 0.004 increased significantly after processing. A 14-fold increase in caffeine levels was also observed. This study provides insight into SEGs as a promising source of industrially important sugars and polyphenols.

  10. Sedimentological and geochemical investigations to understand source of sediments and processes of recent past in Schirmacher Oasis, East Antarctica

    Science.gov (United States)

    Choudhary, Shabnam; Tiwari, Anoop Kumar; Nayak, G. N.; Bejugam, Purnima

    2018-03-01

    Three sediment cores collected from GL-1, V-1(Vetehiya) and L-6 lakes of Schirmacher Oasis, East Antarctica were studied for sediment components (sand, silt, clay, total organic carbon, total nitrogen, TOC/TN ratio and biogenic silica), major elements (Aluminium, Iron and Manganese) and trace metals (Chromium, Zinc, Lead, Cobalt, Cadmium and Nickel). High sand content in all the three cores revealed the release of coarser sediments through mechanical weathering in fluvio-glacial environment. Relatively, high biogenic silica along with high total organic carbon associated with high clay in some sections indicated high primary productivity due to the warming and exposure of the lakes to the ice-melt water influx. TOC/TN ratio for all the cores was found to be < 10 which indicated that the major source of organic matter was autochthonous. Metals were found to be strongly associated with clay and organic carbon in core V-1, with sand and clay in core L-6 while, with silt and organic matter in core GL-1 indicating their role in regulating the distribution of metals. Cadmium in lake GL-1 was found to be associated with total organic carbon and showed largely biogenic origin, while, Cd and Pb in lakes L-6 and V-1 were found to be of anthropogenic origin. All the other metals showed signatures of lithogenic origin.

  11. Dosimetric Comparison of Simulated Human Eye And Water Phantom in Investigation of Iodine Source Effects on Tumour And Healthy Tissues

    International Nuclear Information System (INIS)

    Sadi, A.S.; Masoudi, F.S. K.N.Toosi University of Technology

    2011-01-01

    For better clinical analysis in ophthalmic brachytherapy dosimetry, there is a need for the dose determination in different parts of the eye, so simulating the eye and defining the material of any parts of that, is helpful for better investigating dosimetry in human eye. However in brachytherapy dosimetry, it is common to consider the water phantom as human eye globe. In this work, a full human eye is simulated with MCNP-4C code by considering all parts of the eye like; lens, cornea, retina, choroid, sclera, anterior chamber, optic nerve, bulk of the eye comprising vitreous body and tumour. The average dose in different parts of this full model of human eye is determined and the results are compared with the dose calculated in water phantom. The central axes depth dose and the dose in whole of the tumour for these two simulated eye model are calculated too, and the results are compared. At long last, as the aim of this work is comparing the result of investigating dosimetry between two water phantom as human eye and simulated eye globe, the ratios of the absorbed dose by the healthy tissues to the absorbed dose by the tumour are calculated in these simulations and the comparison between results is done eventually.

  12. Experimental and numerical investigation of thermal radiator performances as a source of heat energy in design of dryer simulation

    Science.gov (United States)

    Wiryanta, I. K. E. H.; Adiaksa, I. M. A.

    2018-01-01

    The purposes of this research was to investigate the temperature performance of tube and fins car radiator experimentally and numerically. The experiment research was carried out on a simulation design consists of a reservoir water tank, a heater, pump to circulate hot water to the radiator and a cooling fan. The hot water mass flow rate is 0.486 kg/s, and the cooling air velocity of the fan is 1 m/s. The heat transfer rate and the effectiveness of radiator were investigated. The results showed that the exhaust heat transfer rate from the radiator tended to increase over time, with an average heat transfer rate of 3974.3 Watt. The maximum heat transfer rate was 4680 Watt obtained at 6 minutes. The effectiveness of the radiator (ε) over time tends to increase with an average of ε = 0.3 and the maximum effectiveness value was obtained at 12 minutes i.e. 0.35. The numerical research conducted using CFD method. The geometry and meshing created using ANSYS Workbench and the post processing using Fluent. The simulation result showed the similarity with the experimental research. The temperatures of air-side radiator are about 45°C.

  13. Building a virtual simulation platform for quasistatic breast ultrasound elastography using open source software: A preliminary investigation.

    Science.gov (United States)

    Wang, Yu; Helminen, Emily; Jiang, Jingfeng

    2015-09-01

    Quasistatic ultrasound elastography (QUE) is being used to augment in vivo characterization of breast lesions. Results from early clinical trials indicated that there was a lack of confidence in image interpretation. Such confidence can only be gained through rigorous imaging tests using complex, heterogeneous but known media. The objective of this study is to build a virtual breast QUE simulation platform in the public domain that can be used not only for innovative QUE research but also for rigorous imaging tests. The main thrust of this work is to streamline biomedical ultrasound simulations by leveraging existing open source software packages including Field II (ultrasound simulator), VTK (geometrical visualization and processing), FEBio [finite element (FE) analysis], and Tetgen (mesh generator). However, integration of these open source packages is nontrivial and requires interdisciplinary knowledge. In the first step, a virtual breast model containing complex anatomical geometries was created through a novel combination of image-based landmark structures and randomly distributed (small) structures. Image-based landmark structures were based on data from the NIH Visible Human Project. Subsequently, an unstructured FE-mesh was created by Tetgen. In the second step, randomly positioned point scatterers were placed within the meshed breast model through an octree-based algorithm to make a virtual breast ultrasound phantom. In the third step, an ultrasound simulator (Field II) was used to interrogate the virtual breast phantom to obtain simulated ultrasound echo data. Of note, tissue deformation generated using a FE-simulator (FEBio) was the basis of deforming the original virtual breast phantom in order to obtain the postdeformation breast phantom for subsequent ultrasound simulations. Using the procedures described above, a full cycle of QUE simulations involving complex and highly heterogeneous virtual breast phantoms can be accomplished for the first time

  14. Tracing melioidosis back to the source: using whole-genome sequencing to investigate an outbreak originating from a contaminated domestic water supply.

    Science.gov (United States)

    McRobb, Evan; Sarovich, Derek S; Price, Erin P; Kaestli, Mirjam; Mayo, Mark; Keim, Paul; Currie, Bart J

    2015-04-01

    Melioidosis, a disease of public health importance in Southeast Asia and northern Australia, is caused by the Gram-negative soil bacillus Burkholderia pseudomallei. Melioidosis is typically acquired through environmental exposure, and case clusters are rare, even in regions where the disease is endemic. B. pseudomallei is classed as a tier 1 select agent by the Centers for Disease Control and Prevention; from a biodefense perspective, source attribution is vital in an outbreak scenario to rule out a deliberate release. Two cases of melioidosis within a 3-month period at a residence in rural northern Australia prompted an investigation to determine the source of exposure. B. pseudomallei isolates from the property's groundwater supply matched the multilocus sequence type of the clinical isolates. Whole-genome sequencing confirmed the water supply as the probable source of infection in both cases, with the clinical isolates differing from the likely infecting environmental strain by just one single nucleotide polymorphism (SNP) each. For the first time, we report a phylogenetic analysis of genomewide insertion/deletion (indel) data, an approach conventionally viewed as problematic due to high mutation rates and homoplasy. Our whole-genome indel analysis was concordant with the SNP phylogeny, and these two combined data sets provided greater resolution and a better fit with our epidemiological chronology of events. Collectively, this investigation represents a highly accurate account of source attribution in a melioidosis outbreak and gives further insight into a frequently overlooked reservoir of B. pseudomallei. Our methods and findings have important implications for outbreak source tracing of this bacterium and other highly recombinogenic pathogens. Copyright © 2015, American Society for Microbiology. All Rights Reserved.

  15. In vivo crystalline lens measurements with novel swept-source optical coherent tomography: an investigation on variability of measurement

    Science.gov (United States)

    Shoji, Takuhei; Kato, Naoko; Ishikawa, Sho; Ibuki, Hisashi; Yamada, Norihiro; Kimura, Itaru; Shinoda, Kei

    2017-01-01

    Objective To evaluate the reproducibility of in vivo crystalline lens measurements obtained by novel commercially available swept-source (SS) optical coherence tomography (OCT) specifically designed for anterior segment imaging. Methods and analysis One eye from each of 30 healthy subjects was randomly selected using the CASIA2 (Tomey, Nagoya, Japan) in two separate visits within a week. Each eye was imaged twice. After image scanning, the anterior and posterior lens curvatures and lens thickness were calculated automatically by the CASIA2 built-in program at 0 dioptre (D) (static), −1 D, −3 D and −5 D accommodative stress. The intraobserver and intervisit reproducibility coefficient (RC) and intraclass correlation coefficient (ICC) were calculated. Results The intraobserver and intervisit RCs ranged from 0.824 to 1.254 mm and 0.789 to 0.911 mm for anterior lens curvature, from 0.276 to 0.299 mm and 0.221 to 0.270 mm for posterior lens curvature and from 0.065 to 0.094 mm and 0.054 to 0.132 mm for lens thickness, respectively. The intraobserver and intervisit ICCs ranged from 0.831 to 0.865 and 0.828 to 0.914 for anterior lens curvature, from 0.832 to 0.898 and 0.840 to 0.933 for posterior lens curvature and from 0.980 to 0.992 and 0.942 to 0.995 for lens thickness. High ICC values were observed for each measurement regardless of accommodative stress. RCs in younger subjects tended to be larger than those in older subjects. Conclusions This novel anterior segment SS-OCT instrument produced reliable in vivo crystalline lens measurement with good repeatability and reproducibility regardless of accommodation stress. PMID:29354706

  16. Bacterial contamination of street vending food in Kumasi, Ghana ...

    African Journals Online (AJOL)

    Street vending foods are readily available sources of meals for many people but the biological safe-ty of such food is always in doubt. The aim of this study is to ascertain bacterial isolate and deter-mine total counts of bacterial species responsible for the contamination of the street vending food in Kumasi so as to determine ...

  17. Solution 1H NMR investigation of the active site molecular and electronic structures of substrate-bound, cyanide-inhibited HmuO, a bacterial heme oxygenase from Corynebacterium diphtheriae.

    Science.gov (United States)

    Li, Yiming; Syvitski, Ray T; Chu, Grace C; Ikeda-Saito, Masao; Mar, Gerd N La

    2003-02-28

    The molecular structure and dynamic properties of the active site environment of HmuO, a heme oxygenase (HO) from the pathogenic bacterium Corynebacterium diphtheriae, have been investigated by (1)H NMR spectroscopy using the human HO (hHO) complex as a homology model. It is demonstrated that not only the spatial contacts among residues and between residues and heme, but the magnetic axes that can be related to the direction and magnitude of the steric tilt of the FeCN unit are strongly conserved in the two HO complexes. The results indicate that very similar contributions of steric blockage of several meso positions and steric tilt of the attacking ligand are operative. A distal H-bond network that involves numerous very strong H-bonds and immobilized water molecules is identified in HmuO that is analogous to that previously identified in hHO (Li, Y., Syvitski, R. T., Auclair, K., Wilks, A., Ortiz de Montellano, P. R., and La Mar, G. N. (2002) J. Biol. Chem. 277, 33018-33031). The NMR results are completely consistent with the very recent crystal structure of the HmuO.substrate complex. The H-bond network/ordered water molecules are proposed to orient the distal water molecule near the catalytically key Asp(136) (Asp(140) in hHO) that stabilizes the hydroperoxy intermediate. The dynamic stability of this H-bond network in HmuO is significantly greater than in hHO and may account for the slower catalytic rate in bacterial HO compared with mammalian HO.

  18. Different senescent HDPE pipe-risk: brief field investigation from source water to tap water in China (Changsha City).

    Science.gov (United States)

    Tang, Jing; Tang, Lin; Zhang, Chang; Zeng, Guangming; Deng, Yaocheng; Dong, Haoran; Wang, Jingjing; Wu, Yanan

    2015-10-01

    Semi-volatile organic compounds (SVOCs) derived from plastic pipes widely used in water distribution definitely influence our daily drinking water quality. There are still few scientific or integrated studies on the release and degradation of the migrating chemicals in pipelines. This investigation was carried out at field sites along a pipeline in Changsha, China. Two chemicals, 2, 4-tert-buthylphenol and 1, 3-diphenylguanidine, were found to be migrating from high density polyethylene (HDPE) pipe material. New pipes released more of these two compounds than older pipes, and microorganisms living in older pipes tended to degrade them faster, indicating that the aged pipes were safer for water transmission. Microorganism degradation in water plays a dominant role in the control of these substances. To minimize the potential harm to human, a more detailed study incorporating assessment of their risk should be carried out, along with seeking safer drinking pipes.

  19. Seismic investigation of the Kunlun Fault: Analysis of the INDEPTH IV 2-D active-source seismic dataset

    Science.gov (United States)

    Seelig, William George

    The Tibetan Plateau has experienced significant crustal thickening and deformation since the continental subduction and collision of the Asian and Indian plates in the Eocene. Deformation of the northern Tibetan Plateau is largely accommodated by strike-slip faulting. The Kunlun Fault is a 1000-km long strike-slip fault near the northern boundary of the Plateau that has experienced five magnitude 7.0 or greater earthquakes in the past 100 years and represents a major rheological boundary. Active-source, 2-D seismic reflection/refraction data, collected as part of project INDEPTH IV (International Deep Profiling of Tibet and the Himalaya, phase IV) in 2007, was used to examine the structure and the dip of the Kunlun fault. The INDEPTH IV data was acquired to better understand the tectonic evolution of the northeastern Tibetan Plateau, such as the far-field deformation associated with the continent-continent collision and the potential subduction of the Asian continent beneath northern Tibet. Seismic reflection common depth point (CDP) stacks were examined to look for reflectivity patterns that may be associated with faulting. A possible reflection from the buried North Kunlun Thrust (NKT) is identified at 18-21 km underneath the East Kunlun Mountains, with an estimated apparent dip of 15°S and thrusting to the north. Minimally-processed shot gathers were also inspected for reflections off near-vertical structures such as faults and information on first-order velocity structure. Shot offset and nearest receiver number to reflection was catalogued to increase confidence of picks. Reflections off the North Kunlun (NKF) and South Kunlun Faults (SKF) were identified and analyzed for apparent dip and subsurface geometry. Fault reflection analysis found that the North Kunlun Fault had an apparent dip of approximately 68ºS to an estimated depth of 5 km, while the South Kunlun Fault dipped at approximately 78ºN to an estimated 3.5 km depth. Constraints on apparent dip and

  20. Changes in Soil Bacterial Communities and Diversity in ...

    Science.gov (United States)

    Silver-induced selective pressure is becoming increasingly important due to the growing use of silver (Ag) as an antimicrobial agent in biomedical and commercial products. With demonstrated links between environmental resistomes and clinical pathogens, it is important to identify microbial profiles related to silver tolerance/resistance. We investigated the effects of ionic Ag stress on soil bacterial communities and identified resistant/persistant bacterial populations. Silver treatments of 50 - 400 mg Ag kg-1 soil were established in five soils. Chemical lability measurements using diffusive gradients in thin-film devices confirmed that significant (albeit decreasing) labile Ag concentrations were present throughout the 9-month incubation period. Synchrotron X-ray absorption near edge structure spectroscopy demonstrate that this decreasing lability was due to changes in Ag speciation to less soluble forms such as Ag0 and Ag2S. Real-time PCR and Illumina MiSeq screening of 16S rRNA bacterial genes showed β-diversity in response to Ag pressure, and immediate and significant reductions in 16S rRNA gene counts with varying degrees of recovery. These effects were more strongly influenced by exposure time than by Ag dose at these rates. Ag-selected dominant OTUs principally resided in known persister taxa (mainly Gram positive), including metal-tolerant bacteria and slow-growing Mycobacteria. Soil microbial communities have been implicated as sources of an

  1. Monte Carlo investigation of the dosimetric properties of the new 103Pd BrachySeedTMPd-103 Model Pd-1 source

    International Nuclear Information System (INIS)

    Chan, Gordon H.; Prestwich, William V.

    2002-01-01

    Recently, 103 Pd brachytherapy sources have been increasingly used for interstitial implants as an alternative to 125 I sources. The BrachySeed TM Pd-103 Model Pd-1 seed is one of the latest in a series of new brachytherapy sources that have become available commercially. The dosimetric properties of the seed were investigated by Monte Carlo simulation, which was performed using the Integrated Tiger Series CYLTRAN code. Following the AAPM Task Group 43 formalism, the dose rate constant, radial dose function, and anisotropy parameters were determined. The dose rate constant, Λ, was calculated to be 0.613±3% cGy h -1 U -1 . This air kerma strength was derived from Monte Carlo simulation using the point extrapolation method. The radial dose function, g(r), was computed at distances from 0.15 to 10 cm. The anisotropy function, F(r,θ), and anisotropy factor, φ an (r), were calculated at distances from 0.5 to 7 cm. The anisotropy constant, φ(bar sign) an , was determined to be 0.978, which is closer to unity than most other 103 Pd seeds, indicating a high degree of uniformity in dose distribution. The dose rate constant and the radial dose function were also investigated by analytical modeling, which served as an independent evaluation of the Monte Carlo data, and found to be in good agreement with the Monte Carlo results

  2. Feather barbs as a good source of mtDNA for bird species identification in forensic wildlife investigations.

    Science.gov (United States)

    Speller, Camilla F; Nicholas, George P; Yang, Dongya Y

    2011-07-28

    The ability to accurately identify bird species is crucial for wildlife law enforcement and bird-strike investigations. However, such identifications may be challenging when only partial or damaged feathers are available for analysis. By applying vigorous contamination controls and sensitive PCR amplification protocols, we found that it was feasible to obtain accurate mitochondrial (mt)DNA-based species identification with as few as two feather barbs. This minimally destructive DNA approach was successfully used and tested on a variety of bird species, including North American wild turkey (Meleagris gallopavo), Canada goose (Branta canadensis), blue heron (Ardea herodias) and pygmy owl (Glaucidium californicum). The mtDNA was successfully obtained from 'fresh' feathers, historic museum specimens and archaeological samples, demonstrating the sensitivity and versatility of this technique. By applying appropriate contamination controls, sufficient quantities of mtDNA can be reliably recovered and analyzed from feather barbs. This previously overlooked substrate provides new opportunities for accurate DNA species identification when minimal feather samples are available for forensic analysis.

  3. Surface chemical analysis and ab initio investigations of CsI coated C fiber cathodes for high power microwave sources

    Science.gov (United States)

    Vlahos, Vasilios; Morgan, Dane; LaCour, Matthew; Golby, Ken; Shiffler, Don; Booske, John H.

    2010-02-01

    CsI coated C fiber cathodes are promising electron emitters utilized in field emission applications. Ab initio calculations, in conjunction with experimental investigations on CsI-spray coated C fiber cathodes, were performed in order to better understand the origin of the low turn-on E-field obtained, as compared to uncoated C fibers. One possible mechanism for lowering the turn-on E-field is surface dipole layers reducing the work function. Ab initio modeling revealed that surface monolayers of Cs, CsI, Cs2O, and CsO are all capable of producing low work function C fiber cathodes (1 eV<Φ<1.5 eV), yielding a reduction in the turn-on E-field by as much as ten times, when compared to the bare fiber. Although a CsI-containing aqueous solution is spray deposited on the C fiber surface, energy dispersive x-ray spectroscopy and scanning auger microscopy measurements show coabsorption of Cs and I into the fiber interior and Cs and O on the fiber surface, with no surface I. It is therefore proposed that a cesium oxide (CsxOy) surface coating is responsible, at least in part, for the low turn E-field and superior emission characteristics of this type of fiber cathode. This CsxOy layer could be formed during preconditioning heating. CsxOy surface layers cannot only lower the fiber work function by the formation of surface dipoles (if they are thin enough) but may also enhance surface emission through their ability to emit secondary electrons due to a process of grazing electron impact. These multiple electron emission processes may explain the reported 10-100 fold reduction in the turn-on E-field of coated C fibers.

  4. Population of computational rabbit-specific ventricular action potential models for investigating sources of variability in cellular repolarisation.

    Directory of Open Access Journals (Sweden)

    Philip Gemmell

    Full Text Available Variability is observed at all levels of cardiac electrophysiology. Yet, the underlying causes and importance of this variability are generally unknown, and difficult to investigate with current experimental techniques. The aim of the present study was to generate populations of computational ventricular action potential models that reproduce experimentally observed intercellular variability of repolarisation (represented by action potential duration and to identify its potential causes. A systematic exploration of the effects of simultaneously varying the magnitude of six transmembrane current conductances (transient outward, rapid and slow delayed rectifier K(+, inward rectifying K(+, L-type Ca(2+, and Na(+/K(+ pump currents in two rabbit-specific ventricular action potential models (Shannon et al. and Mahajan et al. at multiple cycle lengths (400, 600, 1,000 ms was performed. This was accomplished with distributed computing software specialised for multi-dimensional parameter sweeps and grid execution. An initial population of 15,625 parameter sets was generated for both models at each cycle length. Action potential durations of these populations were compared to experimentally derived ranges for rabbit ventricular myocytes. 1,352 parameter sets for the Shannon model and 779 parameter sets for the Mahajan model yielded action potential duration within the experimental range, demonstrating that a wide array of ionic conductance values can be used to simulate a physiological rabbit ventricular action potential. Furthermore, by using clutter-based dimension reordering, a technique that allows visualisation of multi-dimensional spaces in two dimensions, the interaction of current conductances and their relative importance to the ventricular action potential at different cycle lengths were revealed. Overall, this work represents an important step towards a better understanding of the role that variability in current conductances may play in

  5. Population of computational rabbit-specific ventricular action potential models for investigating sources of variability in cellular repolarisation.

    Science.gov (United States)

    Gemmell, Philip; Burrage, Kevin; Rodriguez, Blanca; Quinn, T Alexander

    2014-01-01

    Variability is observed at all levels of cardiac electrophysiology. Yet, the underlying causes and importance of this variability are generally unknown, and difficult to investigate with current experimental techniques. The aim of the present study was to generate populations of computational ventricular action potential models that reproduce experimentally observed intercellular variability of repolarisation (represented by action potential duration) and to identify its potential causes. A systematic exploration of the effects of simultaneously varying the magnitude of six transmembrane current conductances (transient outward, rapid and slow delayed rectifier K(+), inward rectifying K(+), L-type Ca(2+), and Na(+)/K(+) pump currents) in two rabbit-specific ventricular action potential models (Shannon et al. and Mahajan et al.) at multiple cycle lengths (400, 600, 1,000 ms) was performed. This was accomplished with distributed computing software specialised for multi-dimensional parameter sweeps and grid execution. An initial population of 15,625 parameter sets was generated for both models at each cycle length. Action potential durations of these populations were compared to experimentally derived ranges for rabbit ventricular myocytes. 1,352 parameter sets for the Shannon model and 779 parameter sets for the Mahajan model yielded action potential duration within the experimental range, demonstrating that a wide array of ionic conductance values can be used to simulate a physiological rabbit ventricular action potential. Furthermore, by using clutter-based dimension reordering, a technique that allows visualisation of multi-dimensional spaces in two dimensions, the interaction of current conductances and their relative importance to the ventricular action potential at different cycle lengths were revealed. Overall, this work represents an important step towards a better understanding of the role that variability in current conductances may play in experimentally

  6. Bacterial Diversity in Submarine Groundwater along the Coasts of the Yellow Sea.

    Science.gov (United States)

    Ye, Qi; Liu, Jianan; Du, Jinzhou; Zhang, Jing

    2015-01-01

    Submarine groundwater (SGD) is one of the most significant pathways for the exchange of groundwater and/or source of nutrients, metals and carbon to the ocean, subsequently cause deleterious impacts on the coastal ecosystems. Microorganisms have been recognized as the important participators in the biogeochemical processes in the SGD. In this study, by utilizing 16S rRNA-based Illumina Miseq sequencing technology, we investigated bacterial diversity and distribution in both fresh well water and brackish recirculated porewater along the coasts in the Yellow Sea. The results showed that Actinobacteria and Betaproteobacteria, especially Comamonas spp. and Limnohabitans spp. were dominated in fresh well samples. Distinct patterns of bacterial communities were found among the porewater samples due to different locations, for examples, Cyanbacteria was the most abundant in the porewater samples far from the algal bloomed areas. The analysis of correlation between representative bacterial taxonomic groups and the contexture environmental parameters showed that fresh well water and brackish porewater might provide different nutrients to the coastal waters. Potential key bacterial groups such as Comamonas spp. may be excellent candidates for the bioremediation of the natural pollutants in the SGD. Our comprehensive understanding of bacterial diversity in the SGD along the coasts of the Yellow Sea will create a basis for designing the effective clean-up approach in-situ, and provide valuable information for the coastal management.

  7. Bacterial Diversity in Submarine Groundwater along the Coasts of the Yellow Sea

    Directory of Open Access Journals (Sweden)

    Qi eYe

    2016-01-01

    Full Text Available Submarine groundwater (SGD is one of the most significant pathways for the exchange of groundwater and/or source of nutrients, metals and carbon to the ocean, subsequently cause deleterious impacts on the coastal ecosystems. Microorganisms have been recognized as the important participators in the biogeochemical processes in the SGD. In this study, by utilizing 16S rRNA-based Illumina Miseq sequencing technology, we investigated bacterial diversity and distribution in both fresh well water and brackish recirculated porewater along the coasts in the Yellow Sea. The results showed that Actinobacteria and Betaproteobacteria, especially Comamonas spp. and Limnohabitans spp. were dominated in fresh well samples. Distinct patterns of bacterial communities were found among the porewater samples due to different locations, for examples, Cyanbacteria was the most abundant in the porewater samples far from the algal bloomed areas. The analysis of correlation between representative bacterial taxonomic groups and the contexture environmental parameters showed that fresh well water and brackish porewater might provide different nutrients to the coastal waters. Potential key bacterial groups such as Comamonas spp. may be excellent candidates for the bioremediation of the natural pollutants in the SGD. Our comprehensive understanding of bacterial diversity in the SGD along the coasts of the Yellow Sea will create a basis for designing the effective clean-up approach in-situ, and provide valuable information for the coastal management.

  8. Bacterial-Fungal Interactions: Hyphens between Agricultural, Clinical, Environmental, and Food Microbiologists

    Science.gov (United States)

    Frey-Klett, P.; Burlinson, P.; Deveau, A.; Barret, M.; Tarkka, M.; Sarniguet, A.

    2011-01-01

    Summary: Bacteria and fungi can form a range of physical associations that depend on various modes of molecular communication for their development and functioning. These bacterial-fungal interactions often result in changes to the pathogenicity or the nutritional influence of one or both partners toward plants or animals (including humans). They can also result in unique contributions to biogeochemical cycles and biotechnological processes. Thus, the interactions between bacteria and fungi are of central importance to numerous biological questions in agriculture, forestry, environmental science, food production, and medicine. Here we present a structured review of bacterial-fungal interactions, illustrated by examples sourced from many diverse scientific fields. We consider the general and specific properties of these interactions, providing a global perspective across this emerging multidisciplinary research area. We show that in many cases, parallels can be drawn between different scenarios in which bacterial-fungal interactions are important. Finally, we discuss how new avenues of investigation may enhance our ability to combat, manipulate, or exploit bacterial-fungal complexes for the economic and practical benefit of humanity as well as reshape our current understanding of bacterial and fungal ecology. PMID:22126995

  9. Bacterial vaginosis - aftercare

    Science.gov (United States)

    Bacterial vaginosis (BV) is a type of vaginal infection. The vagina normally contains both healthy bacteria and unhealthy bacteria. BV occurs when more unhealthy bacteria grow than healthy bacteria. No one knows ...

  10. Bacterial surface adaptation

    Science.gov (United States)

    Utada, Andrew

    2014-03-01

    Biofilms are structured multi-cellular communities that are fundamental to the biology and ecology of bacteria. Parasitic bacterial biofilms can cause lethal infections and biofouling, but commensal bacterial biofilms, such as those found in the gut, can break down otherwise indigestible plant polysaccharides and allow us to enjoy vegetables. The first step in biofilm formation, adaptation to life on a surface, requires a working knowledge of low Reynolds number fluid physics, and the coordination of biochemical signaling, polysaccharide production, and molecular motility motors. These crucial early stages of biofilm formation are at present poorly understood. By adapting methods from soft matter physics, we dissect bacterial social behavior at the single cell level for several prototypical bacterial species, including Pseudomonas aeruginosa and Vibrio cholerae.

  11. Bacterial intermediate filaments

    DEFF Research Database (Denmark)

    Charbon, Godefroid; Cabeen, M.; Jacobs-Wagner, C.

    2009-01-01

    Crescentin, which is the founding member of a rapidly growing family of bacterial cytoskeletal proteins, was previously proposed to resemble eukaryotic intermediate filament (IF) proteins based on structural prediction and in vitro polymerization properties. Here, we demonstrate that crescentin...

  12. Diagnosis of bacterial infection

    African Journals Online (AJOL)

    direct or indirect evidence of a compatible bacterial pathogen. Inflammation may be .... cardinal features (fever, confusion, headache and neck stiffness). .... specificity, inappropriate indications or poor sampling technique may diminish this ...

  13. An investigation into the accuracy of Acuros(TM) BV in heterogeneous phantoms for a (192)Ir HDR source using LiF TLDs.

    Science.gov (United States)

    Manning, Siobhan; Nyathi, Thulani

    2014-09-01

    The aim of this study was to evaluate the accuracy of the new Acuros(TM) BV algorithm using well characterized LiF:Mg,Ti TLD 100 in heterogeneous phantoms. TLDs were calibrated using an (192)Ir source and the AAPM TG-43 calculated dose. The Tölli and Johansson Large Cavity principle and Modified Bragg Gray principle methods confirm the dose calculated by TG-43 at a distance of 5 cm from the source to within 4 %. These calibrated TLDs were used to measure the dose in heterogeneous phantoms containing air, stainless steel, bone and titanium. The TLD results were compared with the AAPM TG-43 calculated dose and the Acuros calculated dose. Previous studies by other authors have shown a change in TLD response with depth when irradiated with an (192)Ir source. This TLD depth dependence was assessed by performing measurements at different depths in a water phantom with an (192)Ir source. The variation in the TLD response with depth in a water phantom was not found to be statistically significant for the distances investigated. The TLDs agreed with Acuros(TM) BV within 1.4 % in the air phantom, 3.2 % in the stainless steel phantom, 3 % in the bone phantom and 5.1 % in the titanium phantom. The TLDs showed a larger discrepancy when compared to TG-43 with a maximum deviation of 9.3 % in the air phantom, -11.1 % in the stainless steel phantom, -14.6 % in the bone phantom and -24.6 % in the titanium phantom. The results have shown that Acuros accounts for the heterogeneities investigated with a maximum deviation of -5.1 %. The uncertainty associated with the TLDs calibrated in the PMMA phantom is ±8.2 % (2SD).

  14. Investigations concerning the source term for the emission of fission products and transuranic elements from the highly radioactive waste in the temperature region between 200 and 11000C

    International Nuclear Information System (INIS)

    Baumgartner, F.; Krebs, K.; Merte, B.

    1984-04-01

    Starting point of these investigations was the research report 290 of the former Institute for Reactor Safety, Cologne. The largely incorrect assumptions made in the chemical statement led to high radioactive doses not only for the closer, but also for the further surroundings of a reprocessing plant in case that the cooling system of the fuel element storage or the storage tank for the highly radioactive waste (HAW) should become inoperative for an extended period of time. However, as a result of this temperature region which we used in our experiments, the investigations concerning the source term of the HAW between 1100 and 1200 0 C became interesting again, because a glass melt of about 1100 0 C is used during the PAMELA-process for the vitrification of the HAW. The aqueous HAW-solution is thereby poured onto a hot glass melt. The solution thereby dries up, the HAW-salt is calcined and finally sinks into the glass melt. From today's point of view an investigation concerning the source term of the fission products and transuranic elements from the HAW at 1100 0 C therefore provides information regarding the fission product- and transuranic element volatilization within the melting furnace during the vitrification process. The results may now therefore also be considered a contribution for the layout of the off-gas purification system during the PAMELA-process

  15. Nuclear techniques to investigate source and origin of groundwater pollutants and their flow path at Indian Rare Earths Ltd., Cochin, Kerala

    International Nuclear Information System (INIS)

    Tirumalesh, K.; Shivanna, K.; Noble, J.

    2007-01-01

    Hydrochemical, environmental isotope and injected radiotracer investigations were carried out in order to investigate the possible source of contaminants and their movement in groundwater at Indian Rare Earths (IRE) site. Water samples were collected from piezometers, dug wells and river in and around IRE site for measurement of physical parameters, chemistry and isotopes. Chemical results show high fluoride, nitrate, sulphate and phosphate concentrations in piezometers whereas dug wells and river samples are free from contamination. Isotope data indicate that the contaminated groundwaters are enriched in δ 2 H and δ 18 O compared to dug well and river samples. Radiotracer experiments were carried out using single-well and multi-well techniques (radiotracers: 82 Br and 3 H) for determining groundwater filtration velocity and flow direction, respectively. Groundwater filtration velocity was found to be about 1.3 cm/day and flow direction is from south to north. Based on the investigations it can be concluded that groundwater is getting contamination from southern part of IRE campus and the possible source for these contaminants could be the Fertilizer and Chemical of Travancore (FACT) industry. (author)

  16. Integrated geophysical investigations for the delineation of source and subsurface structure associated with hydro-uranium anomaly: A case study from South Purulia Shear Zone (SPSZ), India

    Science.gov (United States)

    Sharma, S. P.; Biswas, A.

    2012-12-01

    South Purulia Shear Zone (SPSZ) is an important region for prospecting of uranium mineralization. Geological studies and hydro-uranium anomaly suggest the presence of Uranium deposit around Raghunathpur village which lies about 8 km north of SPSZ. However, detailed geophysical investigations have not been carried out in this region for investigation of uranium mineralization. Since surface signature of uranium mineralization is not depicted near the location, a deeper subsurface source is expected for hydro uranium anomaly. To delineate the subsurface structure and to investigate the origin of hydro-uranium anomaly present in the area, Vertical Electrical Sounding (VES) using Schlumberger array and Gradient Resistivity Profiling (GRP) were performed at different locations along a profile perpendicular to the South Purulia Shear Zone. Apparent resistivity computed from the measured sounding data at various locations shows a continuously increasing trend. As a result, conventional apparent resistivity data is not able to detect the possible source of hydro uranium anomaly. An innovative approach is applied which depicts the apparent conductivity in the subsurface revealed a possible connection from SPSZ to Raghunathpur. On the other hand resistivity profiling data suggests a low resistive zone which is also characterized by low Self-Potential (SP) anomaly zone. Since SPSZ is characterized by the source of uranium mineralization; hydro-uranium anomaly at Raghunathpur is connected with the SPSZ. The conducting zone has been delineated from SPSZ to Raghunathpur at deeper depths which could be uranium bearing. Since the location is also characterized by a low gravity and high magnetic anomaly zone, this conducting zone is likely to be mineralized zone. Keywords: Apparent resistivity; apparent conductivity; Self Potential; Uranium mineralization; shear zone; hydro-uranium anomaly.

  17. Bacterial biofilm and associated infections

    Directory of Open Access Journals (Sweden)

    Muhsin Jamal

    2018-01-01

    Full Text Available Microscopic entities, microorganisms that drastically affect human health need to be thoroughly investigated. A biofilm is an architectural colony of microorganisms, within a matrix of extracellular polymeric substance that they produce. Biofilm contains microbial cells adherent to one-another and to a static surface (living or non-living. Bacterial biofilms are usually pathogenic in nature and can cause nosocomial infections. The National Institutes of Health (NIH revealed that among all microbial and chronic infections, 65% and 80%, respectively, are associated with biofilm formation. The process of biofilm formation consists of many steps, starting with attachment to a living or non-living surface that will lead to formation of micro-colony, giving rise to three-dimensional structures and ending up, after maturation, with detachment. During formation of biofilm several species of bacteria communicate with one another, employing quorum sensing. In general, bacterial biofilms show resistance against human immune system, as well as against antibiotics. Health related concerns speak loud due to the biofilm potential to cause diseases, utilizing both device-related and non-device-related infections. In summary, the understanding of bacterial biofilm is important to manage and/or to eradicate biofilm-related diseases. The current review is, therefore, an effort to encompass the current concepts in biofilm formation and its implications in human health and disease.

  18. Bacterial Biofilms in Jones Tubes.

    Science.gov (United States)

    Ahn, Eric S; Hauck, Matthew J; Kirk Harris, Jonathan; Robertson, Charles E; Dailey, Roger A

    To investigate the presence and microbiology of bacterial biofilms on Jones tubes (JTs) by direct visualization with scanning electron microscopy and polymerase chain reaction (PCR) of representative JTs, and to correlate these findings with inflammation and/or infection related to the JT. In this study, prospective case series were performed. JTs were recovered from consecutive patients presenting to clinic for routine cleaning or recurrent irritation/infection. Four tubes were processed for scanning electron microscopy alone to visualize evidence of biofilms. Two tubes underwent PCR alone for bacterial quantification. One tube was divided in half and sent for scanning electron microscopy and PCR. Symptoms related to the JTs were recorded at the time of recovery. Seven tubes were obtained. Five underwent SEM, and 3 out of 5 showed evidence of biofilms (60%). Two of the 3 biofilms demonstrated cocci and the third revealed rods. Three tubes underwent PCR. The predominant bacteria identified were Pseudomonadales (39%), Pseudomonas (16%), and Staphylococcus (14%). Three of the 7 patients (43%) reported irritation and discharge at presentation. Two symptomatic patients, whose tubes were imaged only, revealed biofilms. The third symptomatic patient's tube underwent PCR only, showing predominantly Staphylococcus (56%) and Haemophilus (36%) species. Two of the 4 asymptomatic patients also showed biofilms. All symptomatic patients improved rapidly after tube exchange and steroid antibiotic drops. Bacterial biofilms were variably present on