Role of TRIM33 in Wnt signaling during mesendoderm differentiation.

Science.gov (United States)

Xia, Xiaojie; Zuo, Feifei; Luo, Maoguo; Sun, Ye; Bai, Jianbo; Xi, Qiaoran

2017-10-01

Tripartite motif 33 (TRIM33), a member of the transcription intermediate factor 1 (TIF1) family of transcription cofactors, mediates transforming growth factor-beta (TGF-β) signaling through its PHD-Bromo cassette in mesendoderm differentiation during early mouse embryonic development. However, the role of the TRIM33 RING domain in embryonic differentiation is less clear. Here, we report that TRIM33 mediates Wnt signaling by directly regulating the expression of a specific subset of Wnt target genes, and this action is independent of its RING domain. We show that TRIM33 interacts with β-catenin, a central player in Wnt signaling in mouse embryonic stem cells (mESCs). In contrast to previous reports in cancer cell lines, the RING domain does not appear to function as the E3 ligase for β-catenin, since neither knockout nor overexpression of TRIM33 had an effect on β-catenin protein levels in mESCs. Furthermore, we show that although TRIM33 seems to be dispensable for Wnt signaling through a reporter assay, loss of TRIM33 significantly impairs the expression of a subset of Wnt target genes, including Mixl1, in a Wnt signaling-dependent manner. Together, our results indicate that TRIM33 regulates Wnt signaling independent of the E3 ligase activity of its RING domain for β-catenin in mESCs.

Measuring core inflation in India: An asymmetric trimmed mean approach

Directory of Open Access Journals (Sweden)

Naresh Kumar Sharma

2015-12-01

Full Text Available The paper seeks to obtain an optimal asymmetric trimmed mean-based core inflation measure in the class of trimmed mean measures when the distribution of price changes is leptokurtic and skewed to the right for any given period. Several estimators based on asymmetric trimmed mean approach are constructed and estimates generated by use of these estimators are evaluated on the basis of certain established empirical criteria. The paper also provides the method of trimmed mean expression “in terms of percentile score.” This study uses 69 monthly price indices which are constituent components of Wholesale Price Index for the period, April 1994 to April 2009, with 1993–1994 as the base year. Results of the study indicate that an optimally trimmed estimator is found when we trim 29.5% from the left-hand tail and 20.5% from the right-hand tail of the distribution of price changes.

TRIM5α association with cytoplasmic bodies is not required for antiretroviral activity

International Nuclear Information System (INIS)

Song, Byeongwoon; Diaz-Griffero, Felipe; Park, Do Hyun; Rogers, Thomas; Stremlau, Matthew; Sodroski, Joseph

2005-01-01

The tripartite motif (TRIM) protein, TRIM5α, restricts infection by particular retroviruses. Many TRIM proteins form cytoplasmic bodies of unknown function. We investigated the relationship between cytoplasmic body formation and the structure and antiretroviral activity of TRIM5α. In addition to diffuse cytoplasmic staining, the TRIM5α proteins from several primate species were located in cytoplasmic bodies of different sizes; by contrast, TRIM5α from spider monkeys did not form cytoplasmic bodies. Despite these differences, all of the TRIM5α proteins exhibited the ability to restrict infection by particular retroviruses. Treatment of cells with geldanamycin, an Hsp90 inhibitor, resulted in disappearance or reduction of the TRIM5α-associated cytoplasmic bodies, yet exerted little effect on the restriction of retroviral infection. Studies of green fluorescent protein-TRIM5α fusion proteins indicated that no TRIM5α domain is specifically required for association with cytoplasmic bodies. Apparently, the formation of cytoplasmic bodies is not required for the antiretroviral activity of TRIM5α

The molluscan RING-finger protein L-TRIM is essential for neuronal outgrowth

NARCIS (Netherlands)

van Diepen, M. T.; Spencer, G.E.; Minnen, J.; Gouwenberg, Y.; Bouwman, J.G.; Smit, A. B.; van Kesteren, R.E.

2005-01-01

The tripartite motif proteins TRIM-2 and TRIM-3 have been put forward as putative organizers of neuronal outgrowth and structural plasticity. Here, we identified a molluscan orthologue of TRIM-2/3, named L-TRIM, which is up-regulated during in vitro neurite outgrowth of central neurons. In adult

Wrinkling reduction of membrane structure by trimming edges

Directory of Open Access Journals (Sweden)

Mingjun Liu

2017-05-01

Full Text Available Thin membranes have negligible bending stiffness, compressive stresses inevitably lead to wrinkling. Therefore, it is important to keep the surface of membrane structures flat in order to guarantee high precision. Edge-trimming is an effective method to passively diminish wrinkles, however a key difficulty in this process is the determination of the optimal trimming level. In this paper, regular polygonal membrane structures subjected to equal radial forces were analyzed, and a new stress field distribution model for arc-edge square membrane structure was proposed to predict the optimal trimming level. This model is simple and applicable to any polygonal membrane structures. Comparison among the results of the finite element analysis, and the experimental and analytical results showed that the proposed model accurately described the stress field distribution and guaranteed that there are no wrinkles appear inside the effective inscribed circle region for the optimal trimming level.

Utilization of smoked salmon trim in extruded smoked salmon jerky.

Science.gov (United States)

Kong, J; Dougherty, M P; Perkins, L B; Camire, M E

2012-06-01

During smoked salmon processing, the dark meat along the lateral line is removed before packaging; this by-product currently has little economic value. In this study, the dark meat trim was incorporated into an extruded jerky. Three formulations were processed: 100% smoked trim, 75% : 25% smoked trim : fresh salmon fillet, and 50% : 50% smoked trim : fresh salmon blends (w/w basis). The base formulation contained salmon (approximately 83.5%), tapioca starch (8%), pregelatinized potato starch (3%), sucrose (4%), salt (1.5%), sodium nitrate (0.02%), and ascorbyl palmitate (0.02% of the lipid content). Blends were extruded in a laboratory-scale twin-screw extruder and then hot-smoked for 5 h. There were no significant differences among formulations in moisture, water activity, and pH. Protein was highest in the 50 : 50 blend jerky. Ash content was highest in the jerky made with 100% trim. Total lipids and salt were higher in the 100% trim jerky than in the 50 : 50 blend. Hot smoking did not adversely affect docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) content in lipids from 100% smoked trim jerky. Servings of salmon jerky made with 75% and 100% smoked trim provided at least 500 mg of EPA and DHA. The 50 : 50 formulation had the highest Intl. Commission on Illumination (CIE) L*, a*, and b* color values. Seventy consumers rated all sensory attributes as between "like slightly" and "like moderately." With some formulation and processing refinements, lateral line trim from smoked salmon processors has potential to be incorporated into acceptable, healthful snack products. Dark meat along the lateral line is typically discarded by smoked salmon processors. This omega-3 fatty acid rich by-product can be used to make a smoked salmon jerky that provides a convenient source of these healthful lipids for consumers. © 2012 Institute of Food Technologists®

Microstructural investigations of the trimmed edge of DP980 steel sheets

Science.gov (United States)

Bhattacharya, S.; Green, D. E.; Sohmshetty, R.; Alpas, A. T.

2017-10-01

In order to reduce vehicle weight while maintaining crashworthiness, advanced high strength steels (AHSSs), such as DP980, are extensively used for manufacturing automotive body components. During trimming operations, the high tensile strength of DP980 sheets tends to cause damage of the trim edge of D2 die inserts, which result in deterioration of the edge quality. The objective of this work is to study the damage microstructures at the trimmed edge of DP980 steel sheets as a function of the number of trimming cycles. A mechanical press equipped with AISI D2 tool steel inserts was used to continuously trim 1.4 mm thick sheets of DP980 at a rate of 30 strokes/min. Cross-sectional SEM images of the trimmed edges revealed that the sheared edge quality of the DP980 sheets decreased, indicated by an increase in the burr width, with an increase in the number of trims from 40,000 to 70,000. Plastic strains were estimated using the displacements of the martensite plates within plastic flow fields of ferrite. Site-specific cross-sectional TEM samples, excised from the trimmed edge using the in-situ `lift-out' technique by focused ion-beam (FIB)-milling, revealed cracking at the ferrite/martensite interfaces after 70,000 cycles indicating an increase in the depth of deformation zone possibly due to trimming with a chipped and blunted die edge.

General Model for Retroviral Capsid Pattern Recognition by TRIM5 Proteins.

Science.gov (United States)

Wagner, Jonathan M; Christensen, Devin E; Bhattacharya, Akash; Dawidziak, Daria M; Roganowicz, Marcin D; Wan, Yueping; Pumroy, Ruth A; Demeler, Borries; Ivanov, Dmitri N; Ganser-Pornillos, Barbie K; Sundquist, Wesley I; Pornillos, Owen

2018-02-15

Restriction factors are intrinsic cellular defense proteins that have evolved to block microbial infections. Retroviruses such as HIV-1 are restricted by TRIM5 proteins, which recognize the viral capsid shell that surrounds, organizes, and protects the viral genome. TRIM5α uses a SPRY domain to bind capsids with low intrinsic affinity ( K D of >1 mM) and therefore requires higher-order assembly into a hexagonal lattice to generate sufficient avidity for productive capsid recognition. TRIMCyp, on the other hand, binds HIV-1 capsids through a cyclophilin A domain, which has a well-defined binding site and higher affinity ( K D of ∼10 μM) for isolated capsid subunits. Therefore, it has been argued that TRIMCyp proteins have dispensed with the need for higher-order assembly to function as antiviral factors. Here, we show that, consistent with its high degree of sequence similarity with TRIM5α, the TRIMCyp B-box 2 domain shares the same ability to self-associate and facilitate assembly of a TRIMCyp hexagonal lattice that can wrap about the HIV-1 capsid. We also show that under stringent experimental conditions, TRIMCyp-mediated restriction of HIV-1 is indeed dependent on higher-order assembly. Both forms of TRIM5 therefore use the same mechanism of avidity-driven capsid pattern recognition. IMPORTANCE Rhesus macaques and owl monkeys are highly resistant to HIV-1 infection due to the activity of TRIM5 restriction factors. The rhesus macaque TRIM5α protein blocks HIV-1 through a mechanism that requires self-assembly of a hexagonal TRIM5α lattice around the invading viral core. Lattice assembly amplifies very weak interactions between the TRIM5α SPRY domain and the HIV-1 capsid. Assembly also promotes dimerization of the TRIM5α RING E3 ligase domain, resulting in synthesis of polyubiquitin chains that mediate downstream steps of restriction. In contrast to rhesus TRIM5α, the owl monkey TRIM5 homolog, TRIMCyp, binds isolated HIV-1 CA subunits much more tightly

Improvement of Springback Predictability after forming and trimming

NARCIS (Netherlands)

Avetisyan, Marianna; Meinders, Vincent T.; Huetink, Han; Storen, S.

2004-01-01

When a sheet metal forming operation, such as deep drawing, is followed by a trimming operation the numerical springback after trimming is influenced by physical parameters and loss of product stiffness due to cutting off the material. Moreover, it is affected by the finite element mesh disturbance

Cyclophilin A potentiates TRIM5α inhibition of HIV-1 nuclear import without promoting TRIM5α binding to the viral capsid.

Directory of Open Access Journals (Sweden)

Mallori Burse

Full Text Available The host immunophilin cyclophilin A (CypA binds to the capsid protein (CA of HIV-1 and regulates its infectivity. Depending on the target cell type, CypA can either promote or inhibit HIV-1 infection. The ability of CypA to promote HIV-1 infection has been extensively studied and linked to several steps in early replication including uncoating, reverse transcription and nuclear import. By contrast, the mechanism by which CypA inhibits infection is less well understood. We investigated the mechanism by which CypA potentiates restriction of HIV-1 by the tripartite motif-containing protein 5 (TRIM5α. Depletion of TRIM5α in the African green monkey cell line Vero, resulted in a loss of inhibition of infection by CypA, demonstrating that inhibition by CypA is mediated by TRIM5α. Complementary genetic and biochemical assays failed to demonstrate an ability of CypA to promote binding of TRIM5α to the viral capsid. TRIM5α inhibits HIV-1 reverse transcription in a proteasome-dependent manner; however, we observed that inhibition of proteasome activity did not reduce the ability of CypA to inhibit infection, suggesting that CypA acts at a step after reverse transcription. Accordingly, we observed a CypA-dependent reduction in the accumulation of nuclear HIV-1 DNA, indicating that CypA specifically promotes TRIM5α inhibition of HIV-1 nuclear import. We also observed that the ability of CypA to inhibit HIV-1 infection is abolished by amino acid substitutions within the conserved CPSF6-binding surface in CA. Our results indicate that CypA inhibits HIV-1 infection in Vero cells not by promoting TRIM5α binding to the capsid but by blocking nuclear import of the HIV-1 preintegration complex.

A Methodology for Integrating Maintainability Using Software Metrics

OpenAIRE

Lewis, John A.; Henry, Sallie M.

1989-01-01

Maintainability must be integrated into software early in the development process. But for practical use, the techniques used must be as unobtrusive to the existing software development process as possible. This paper defines a methodology for integrating maintainability into large-scale software and describes an experiment which implemented the methodology into a major commercial software development environment.

Loss of heterozygosity of TRIM3 in malignant gliomas

International Nuclear Information System (INIS)

Boulay, Jean-Louis; Stiefel, Urs; Taylor, Elisabeth; Dolder, Béatrice; Merlo, Adrian; Hirth, Frank

2009-01-01

Malignant gliomas are frequent primary brain tumors associated with poor prognosis and very limited response to conventional chemo- and radio-therapies. Besides sharing common growth features with other types of solid tumors, gliomas are highly invasive into adjacent brain tissue, which renders them particularly aggressive and their surgical resection inefficient. Therefore, insights into glioma formation are of fundamental interest in order to provide novel molecular targets for diagnostic purposes and potential anti-cancer drugs. Human Tripartite motif protein 3 (TRIM3) encodes a structural homolog of Drosophila brain tumor (brat) implicated in progenitor cell proliferation control and cancer stem cell suppression. TRIM3 is located within the loss of allelic heterozygosity (LOH) hotspot of chromosome segment 11p15.5, indicating a potential role in tumor suppression. ... Here we analyze 70 primary human gliomas of all types and grades and report somatic deletion mapping as well as single nucleotide polymorphism analysis together with quantitative real-time PCR of chromosome segment 11p15.5. Our analysis identifies LOH in 17 cases (24%) of primary human glioma which defines a common 130 kb-wide interval within the TRIM3 locus as a minimal area of loss. We further detect altered genomic dosage of TRIM3 in two glioma cases with LOH at 11p15.5, indicating homozygous deletions of TRIM3. Loss of heterozygosity of chromosome segment 11p15.5 in malignant gliomas suggests TRIM3 as a candidate brain tumor suppressor gene

Printed board dipole trim magnet design for 20 MeV LIA

Energy Technology Data Exchange (ETDEWEB)

Liu, Chengjun; Zhu, Wenjun; Zhang, Kaizhi; Zhang, Wenwei; Yu, Haijun [China Academy of Engineering Physics., Chengdu (China). Inst. of Fluid Physics

1997-12-31

The printed board dipole trim magnet design for a 20 MeV LIA is presented. The prototype dipole magnet with the sin/cos distributed windings has demonstrated more than 650 Gs-cm integrated dipole field and 1% integrated dipole field homogeneity within 5 cm in radius, which is about 40% of the magnet radius. Numerical modeling of two prototype magnet designs using the 3D magnetic field code SCMAG is presented as well as data from magnetic field measurements of the two magnets. The agreement between the calculations and measurements is accurate to 2-3%. (author). 3 figs., 4 refs.

Application of Various Lasers to Laser Trimming Resistance System

Institute of Scientific and Technical Information of China (English)

SUN Ji-feng

2007-01-01

Though the laser trimming resistance has been an old laser machining industry for over 30 years, the development of technology brings new alternative lasers which can be used for the traditional machining. The paper describes application of various lasers to laser trimming resistance system including early traditional krypton arc lamp pumped Nd:YAG to laser, modern popular diode pumped solid state laser and the present advanced harmonic diode pumped solid state laser. Using the new alternative lasers in the laser trimming resistance system can dramatically improve the yields and equipment performance.

Non-local means filter for trim statics

KAUST Repository

Huang, Yunsong

2014-08-05

Structures will be mispositioned across prestack migration gathers in the presence of inaccuracies in the velocity model. Stacking these misaligned gathers runs the risk of destroying important structures in the stacked migration image. To mitigate this problem, we propose a trim statics inspired by the non-local means algorithm originally developed for image denoising. This method differs from the conventional one in two fundamental respects. First, the trim statics are computed by comparing image patches instead of individual image traces. Second, no global pilot trace is needed because only two migration images at a time participate in trim statics and are stacked into one image. A multitude of migration images are stacked recursively in this two-to-one fashion. Tests with a Gulf of Mexico dataset show a noticeable improvement in the feature coherency of the stacked migration image.

Trim waste minimization at the Pinellas Plant

International Nuclear Information System (INIS)

DeLaneuville, D.

1992-01-01

Bacteria counts and several methods of slowing bacterial growth in machine trim coolant are suggested to reduce the frequency of coolant replacement without risking employee health or the longevity of the product or machinery. On-site treatment and disposal of waste trim are recommended to further reduce waste volume. This paper discusses the benefits of these efforts, including projected cost savings based on partial implementation at the Department of Energy's Pinellas Plant

Loss of heterozygosity of TRIM3 in malignant gliomas

Directory of Open Access Journals (Sweden)

Dolder Béatrice

2009-02-01

Full Text Available Abstract Background Malignant gliomas are frequent primary brain tumors associated with poor prognosis and very limited response to conventional chemo- and radio-therapies. Besides sharing common growth features with other types of solid tumors, gliomas are highly invasive into adjacent brain tissue, which renders them particularly aggressive and their surgical resection inefficient. Therefore, insights into glioma formation are of fundamental interest in order to provide novel molecular targets for diagnostic purposes and potential anti-cancer drugs. Human Tripartite motif protein 3 (TRIM3 encodes a structural homolog of Drosophila brain tumor (brat implicated in progenitor cell proliferation control and cancer stem cell suppression. TRIM3 is located within the loss of allelic heterozygosity (LOH hotspot of chromosome segment 11p15.5, indicating a potential role in tumor suppression. ... Methods Here we analyze 70 primary human gliomas of all types and grades and report somatic deletion mapping as well as single nucleotide polymorphism analysis together with quantitative real-time PCR of chromosome segment 11p15.5. Results Our analysis identifies LOH in 17 cases (24% of primary human glioma which defines a common 130 kb-wide interval within the TRIM3 locus as a minimal area of loss. We further detect altered genomic dosage of TRIM3 in two glioma cases with LOH at 11p15.5, indicating homozygous deletions of TRIM3. Conclusion Loss of heterozygosity of chromosome segment 11p15.5 in malignant gliomas suggests TRIM3 as a candidate brain tumor suppressor gene.

A brief history of TRIM5alpha.

Science.gov (United States)

Newman, Ruchi M; Johnson, Welkin E

2007-01-01

In spite of the fact that the first isolates of HIV-1 became available more than 20 years ago, there is still no robust animal model for HIV-1 replication and pathogenesis. This is largely due to the existence of multiple genetic barriers to HIV-1 replication in most nonhuman primates, including a severe block targeting the early, post-entry phase of the viral replication cycle. It is now known that a protein called TRIM5alpha mediates this early restriction in nonhuman primate cells. Tissue culture experiments, together with genetic association studies involving multiple HIV/AIDS cohorts, indicate that the human orthologue of TRIM5alpha does not have a significant impact on HIV-1 replication. However, most human alleles encode a functional protein that can restrict at least one retrovirus unrelated to HIV-1 (N-tropic murine leukemia virus), although one deleterious mutation (H43Y) is present at high frequency in human populations. Phylogenetic analyses of the TRIM5 locus reveal that prehistoric retroviral epidemics, not unlike the current HIV/AIDS pandemic, played a significant role in the evolutionary history of humans and their primate relatives. The discovery of TRIM5alpha's antiretroviral activity sparked the imaginations of many laboratories, and considerable effort has now been channeled into characterizing the protein and determining its possible mechanism(s) of action. It is hoped that research on TRIM5alpha will contribute to the establishment of new and improved models for HIV replication and AIDS pathogenesis, point the way towards novel therapeutic targets to stem the tide of the human AIDS epidemic, provide an experimental window onto the early, post-entry stages of the retroviral replication cycle, and even inspire the search for other cellular factors that modulate retroviral infection.

Compensation of the AKT signaling by ERK signaling in transgenic mice hearts overexpressing TRIM72

Energy Technology Data Exchange (ETDEWEB)

Ham, Young-Mi, E-mail: youngmi_ham@hms.harvard.edu [College of Life Science and Biotechnology, Korea University, Seoul (Korea, Republic of); Department of Cell Biology, Harvard Medical School, Boston, MA 02115 (United States); Mahoney, Sarah Jane [Department of Cell Biology, Harvard Medical School, Boston, MA 02115 (United States)

2013-06-10

The AKT and ERK signaling pathways are known to be involved in cell hypertrophy, proliferation, survival and differentiation. Although there is evidence for crosstalk between these two signaling pathways in cellulo, there is less evidence for cross talk in vivo. Here, we show that crosstalk between AKT and ERK signaling in the hearts of TRIM72-overexpressing transgenic mice (TRIM72-Tg) with alpha-MHC promoter regulates and maintains their heart size. TRIM72, a heart- and skeletal muscle-specific protein, downregulates AKT-mTOR signaling via IRS-1 degradation and reduces the size of rat cardiomyocytes and the size of postnatal TRIM72-Tg hearts. TRIM72 expression was upregulated by hypertrophic inducers in cardiomyocytes, while IRS-1 was downregulated by IGF-1. TRIM72 specifically regulated IGF-1-dependent AKT-mTOR signaling, resulting in a reduction of the size of cardiomyocytes. Postnatal TRIM72-Tg hearts were smaller than control-treated hearts with inhibition of AKT-mTOR signaling. However, adult TRIM72-Tg hearts were larger than of control despite the suppression of AKT-mTOR signaling. Activation of ERK, PKC-α, and JNK were observed to be elevated in adult TRIM72-Tg, and these signals were mediated by ET-1 via the ET receptors A and B. Altogether, these results suggest that AKT signaling regulates cardiac hypertrophy in physiological conditions, and ERK signaling compensates for the absence of AKT signaling during TRIM72 overexpression, leading to pathological hypertrophy. -- Highlights: • TRIM72 inhibits AKT signaling through ubiquitination of IRS-1 in cardiac cells. • TRIM72 regulates the size of cardiac cells. • TRIM72 regulates size of postnatal TRIM72-overexpressing transgenic mice hearts. • Adult TRIM72-overexpressing transgenic mice hearts showed cardiac dysfunction. • Adult TRIM72 transgenic mice hearts showed higher expression of endothelin receptors.

Compensation of the AKT signaling by ERK signaling in transgenic mice hearts overexpressing TRIM72

International Nuclear Information System (INIS)

Ham, Young-Mi; Mahoney, Sarah Jane

2013-01-01

The AKT and ERK signaling pathways are known to be involved in cell hypertrophy, proliferation, survival and differentiation. Although there is evidence for crosstalk between these two signaling pathways in cellulo, there is less evidence for cross talk in vivo. Here, we show that crosstalk between AKT and ERK signaling in the hearts of TRIM72-overexpressing transgenic mice (TRIM72-Tg) with alpha-MHC promoter regulates and maintains their heart size. TRIM72, a heart- and skeletal muscle-specific protein, downregulates AKT-mTOR signaling via IRS-1 degradation and reduces the size of rat cardiomyocytes and the size of postnatal TRIM72-Tg hearts. TRIM72 expression was upregulated by hypertrophic inducers in cardiomyocytes, while IRS-1 was downregulated by IGF-1. TRIM72 specifically regulated IGF-1-dependent AKT-mTOR signaling, resulting in a reduction of the size of cardiomyocytes. Postnatal TRIM72-Tg hearts were smaller than control-treated hearts with inhibition of AKT-mTOR signaling. However, adult TRIM72-Tg hearts were larger than of control despite the suppression of AKT-mTOR signaling. Activation of ERK, PKC-α, and JNK were observed to be elevated in adult TRIM72-Tg, and these signals were mediated by ET-1 via the ET receptors A and B. Altogether, these results suggest that AKT signaling regulates cardiac hypertrophy in physiological conditions, and ERK signaling compensates for the absence of AKT signaling during TRIM72 overexpression, leading to pathological hypertrophy. -- Highlights: • TRIM72 inhibits AKT signaling through ubiquitination of IRS-1 in cardiac cells. • TRIM72 regulates the size of cardiac cells. • TRIM72 regulates size of postnatal TRIM72-overexpressing transgenic mice hearts. • Adult TRIM72-overexpressing transgenic mice hearts showed cardiac dysfunction. • Adult TRIM72 transgenic mice hearts showed higher expression of endothelin receptors

Neuroanatomy-based matrix-guided trimming protocol for the rat brain.

Science.gov (United States)

Defazio, Rossella; Criado, Ana; Zantedeschi, Valentina; Scanziani, Eugenio

2015-02-01

Brain trimming through defined neuroanatomical landmarks is recommended to obtain consistent sections in rat toxicity studies. In this article, we describe a matrix-guided trimming protocol that uses channels to reproduce coronal levels of anatomical landmarks. Both setup phase and validation study were performed on Han Wistar male rats (Crl:WI(Han)), 10-week-old, with bodyweight of 298 ± 29 (SD) g, using a matrix (ASI-Instruments(®), Houston, TX) fitted for brains of rats with 200 to 400 g bodyweight. In the setup phase, we identified eight channels, that is, 6, 8, 10, 12, 14, 16, 19, and 21, matching the recommended landmarks midway to the optic chiasm, frontal pole, optic chiasm, infundibulum, mamillary bodies, midbrain, middle cerebellum, and posterior cerebellum, respectively. In the validation study, we trimmed the immersion-fixed brains of 60 rats using the selected channels to determine how consistently the channels reproduced anatomical landmarks. Percentage of success (i.e., presence of expected targets for each level) ranged from 89 to 100%. Where 100% success was not achieved, it was noted that the shift in brain trimming was toward the caudal pole. In conclusion, we developed and validated a trimming protocol for the rat brain that allow comparable extensiveness, homology, and relevance of coronal sections as the landmark-guided trimming with the advantage of being quickly learned by technicians. © 2014 by The Author(s).

On the optimal trimming of high-throughput mRNA sequence data

Directory of Open Access Journals (Sweden)

Matthew D MacManes

2014-01-01

Full Text Available The widespread and rapid adoption of high-throughput sequencing technologies has afforded researchers the opportunity to gain a deep understanding of genome level processes that underlie evolutionary change, and perhaps more importantly, the links between genotype and phenotype. In particular, researchers interested in functional biology and adaptation have used these technologies to sequence mRNA transcriptomes of specific tissues, which in turn are often compared to other tissues, or other individuals with different phenotypes. While these techniques are extremely powerful, careful attention to data quality is required. In particular, because high-throughput sequencing is more error-prone than traditional Sanger sequencing, quality trimming of sequence reads should be an important step in all data processing pipelines. While several software packages for quality trimming exist, no general guidelines for the specifics of trimming have been developed. Here, using empirically derived sequence data, I provide general recommendations regarding the optimal strength of trimming, specifically in mRNA-Seq studies. Although very aggressive quality trimming is common, this study suggests that a more gentle trimming, specifically of those nucleotides whose Phred score < 2 or < 5, is optimal for most studies across a wide variety of metrics.

TRIM45 negatively regulates NF-κB-mediated transcription and suppresses cell proliferation

International Nuclear Information System (INIS)

Shibata, Mio; Sato, Tomonobu; Nukiwa, Ryota; Ariga, Tadashi; Hatakeyama, Shigetsugu

2012-01-01

Highlights: ► NF-κB plays an important role in cell survival and carcinogenesis. ► TRIM45 negatively regulates TNFα-induced NF-κB-mediated transcription. ► TRIM45 overexpression suppresses cell growth. ► TRIM45 acts as a repressor for the NF-κB signal and regulates cell growth. -- Abstract: The NF-κB signaling pathway plays an important role in cell survival, immunity, inflammation, carcinogenesis, and organogenesis. Activation of NF-κB is regulated by several posttranslational modifications including phosphorylation, neddylation and ubiquitination. The NF-κB signaling pathway is activated by two distinct signaling mechanisms and is strictly modulated by the ubiquitin–proteasome system. It has been reported that overexpression of TRIM45, one of the TRIM family ubiquitin ligases, suppresses transcriptional activities of Elk-1 and AP-1, which are targets of the MAPK signaling pathway. In this study, we showed that TRIM45 also negatively regulates TNFα-induced NF-κB-mediated transcription by a luciferase reporter assay and that TRIM45 lacking a RING domain also has an activity to inhibit the NF-κB signal. Moreover, we found that TRIM45 overexpression suppresses cell growth. These findings suggest that TRIM45 acts as a repressor for the NF-κB signal and regulates cell growth.

Performance of trim coils made by a novel method

International Nuclear Information System (INIS)

Wanderer, P.; Anerella, M.; Cottingham, J.; Ganetis, G.; Garber, M.; Ghosh, A.; Goodzeit, C.; Greene, A.; Gupta, R.; Herrera, J.; Kahn, S.; Kelly, E.; Meade, A.; Morgan, G.; Muratore, J.; Prodell, A.; Rehak, M.; Rohrer, E.P.; Sampson, W.; Shutt, R.; Skaritka, J.; Thompson, P.; Willen, E.

1991-01-01

A precision, automated method of manufacturing trim coils based on printed circuit technology has been developed. Excellent quench performance and increased radiation resistance have been achieved in recently-tested models of sextupole trim coils developed for operation inside 40 mm-aperture SSC Main Collider dipoles. 6 refs., 2 figs

METHODOLOGY FRAMEWORK FOR PROCESS INTEGRATION AND SERVICE MANAGEMENT

Directory of Open Access Journals (Sweden)

Darko Galinec

2007-06-01

Full Text Available History of information systems development was driven by business system's functions automation and mergers and acquisitions - business subjects integration into a whole. Modern business requires business processes integration through their dynamics and thus enterprise application integration (EAI as well. In this connection it is necessary to find ways and means of application integration and interaction in a consistent and reliable way. The real-time enterprise (RTE monitors, captures and analyzes root causes and overt events that are critical to its success the instant those events occur [6]. EAI is determined by business needs and business requirements. It must be based on business process repository and models, business integration methodology (BIM and information flow as well. Decisions concerning technology must be in function of successful application integration. In this paper EAI methodological framework and technological concepts for its achievements are introduced.

TRIM37 defective in mulibrey nanism is a novel RING finger ubiquitin E3 ligase

International Nuclear Information System (INIS)

Kallijaervi, Jukka; Lahtinen, Ulla; Haemaelaeinen, Riikka; Lipsanen-Nyman, Marita; Palvimo, Jorma J.; Lehesjoki, Anna-Elina

2005-01-01

Mulibrey nanism is an autosomal recessive prenatal-onset growth disorder characterized by dysmorphic features, cardiomyopathy, and hepatomegaly. Mutations in TRIM37 encoding a tripartite motif (TRIM, RING-B-box-coiled-coil)-family protein underlie mulibrey nanism. We investigated the ubiquitin ligase activity predicted for the RING domain of TRIM37 by analyzing its autoubiquitination. Full-length TRIM37 and its TRIM domain were highly polyubiquitinated when co-expressed with ubiquitin. Polyubiquitination was decreased in a mutant protein with disrupted RING domain (Cys35Ser;Cys36Ser) and in the Leu76Pro mutant protein, a disease-associated missense mutation affecting the TRIM domain of TRIM37. Bacterially produced GST-TRIM domain fusion protein, but not its Cys35Ser;Cys36Ser or Leu76Pro mutants, were polyubiquitinated in cell-free conditions, implying RING-dependent modification. Ubiquitin was also identified as an interaction partner for TRIM37 in a yeast two-hybrid screen. Ectopically expressed TRIM37 rapidly formed aggregates that were ubiquitin-, proteasome subunit-, and chaperone-positive in immunofluorescence analysis, defining them as aggresomes. The Cys35Ser;Cys36Ser mutant and the Leu76Pro and Gly322Val patient mutant proteins were markedly less prone to aggregation, implying that aggresomal targeting reflects a physiological function of TRIM37. These findings suggest that TRIM37 acts as a TRIM domain-dependent E3 ubiquitin ligase and imply defective ubiquitin-dependent degradation of an as-yet-unidentified target protein in the pathogenesis of mulibrey nanism

Effects of high voltage pulse trimming on structural properties of thick-film resistors

Directory of Open Access Journals (Sweden)

Stanimirović Zdravko

2017-01-01

Full Text Available Nowadays, compact and reliable electronic devices including up-to-date ceramic micro-electro-mechanical systems require thick-film resistors with significantly reduced dimensions and stable and precise resistance values. For that reason, instead of standard laser trimming method, high voltage pulse trimming of thick-film resistors is being introduced. This method allows controlled and reliable resistance adjustment regardless of resistor position or dimensions and without the presence of cuts. However, it causes irreversible structural changes in the pseudorandom network formed during sintering causing the changes in conducting mechanisms. In this paper results of the experimental investigation of high voltage pulse trimming of thick-film resistors are presented. Obtained results are analyzed and correlations between resistance and low-frequency noise changes and changes in conducting mechanisms in resistors due to high voltage pulse trimming are observed. Sources of measured fluctuations are identified and it is shown that this type of trimming is a valid alternative trimming method to the dominant laser trimming. [Project of the Serbian Ministry of Education, Science and Technological Development, Grant no. III44003 and III45007

Genetic parameters for claw disorders and the effect of preselecting cows for trimming.

Science.gov (United States)

van der Spek, D; van Arendonk, J A M; Vallée, A A A; Bovenhuis, H

2013-09-01

Claw disorders are important traits relevant to dairy cattle breeding from an economical and welfare point of view. Selection for reduced claw disorders can be based on hoof trimmer records. Typically, not all cows in a herd are trimmed. Our objectives were to estimate heritabilities and genetic correlations for claw disorders and investigate the effect of selecting cows for trimming. The data set contained 50,238 cows, of which 20,474 cows had at least one claw trimming record, with a total of 29,994 records. Six claw trimmers scored 14 different claw disorders: abscess (AB), corkscrew claw (CC), (inter-)digital dermatitis or heel erosion (DER), double sole (DS), hardship groove (HG), interdigital hyperplasia (IH), interdigital phlegmon (IP), sand crack (SC), super-foul (SF), sole hemorrhage (SH), sole injury (SI), sole ulcer (SU), white line separation (WLS), yellow discoloration of the sole (YD), and a combined claw disorder trait. Frequencies of the claw disorders for trimmed cows ranged from 0.1% (CC, YD, HG) to 23.8% (DER). More than half of the cows scored had at least one claw disorder. Heritability on the observed scale ranged from 0.02 (DS, SH) to 0.14 (IH) and on the underlying scale from 0.05 to 0.43 in trimmed cows. Genetic correlations between laminitis-related claw disorders were moderate to high, and the same was found for hygiene-related claw disorders. The effect of selecting cows for trimming was first investigated by including untrimmed cows in the analyses and assuming they were not affected by claw disorders. Heritabilities on the underlying scale showed only minor changes. Second, different subsets of the data were created based on the percentage of trimmed cows in the herd. Heritabilities for IH, DER, and SU tended to decrease when a higher percentage of cows in the herd were trimmed. Finally, a bivariate model with a claw disorder and the trait "trimming status" was used, but heritabilities were similar. Heritability for trimming status was

SUMO-interacting motifs of human TRIM5α are important for antiviral activity.

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Gloria Arriagada

2011-04-01

Full Text Available Human TRIM5α potently restricts particular strains of murine leukemia viruses (the so-called N-tropic strains but not others (the B- or NB-tropic strains during early stages of infection. We show that overexpression of SUMO-1 in human 293T cells, but not in mouse MDTF cells, profoundly blocks N-MLV infection. This block is dependent on the tropism of the incoming virus, as neither B-, NB-, nor the mutant R110E of N-MLV CA (a B-tropic switch are affected by SUMO-1 overexpression. The block occurred prior to reverse transcription and could be abrogated by large amounts of restricted virus. Knockdown of TRIM5α in 293T SUMO-1-overexpressing cells resulted in ablation of the SUMO-1 antiviral effects, and this loss of restriction could be restored by expression of a human TRIM5α shRNA-resistant plasmid. Amino acid sequence analysis of human TRIM5α revealed a consensus SUMO conjugation site at the N-terminus and three putative SUMO interacting motifs (SIMs in the B30.2 domain. Mutations of the TRIM5α consensus SUMO conjugation site did not affect the antiviral activity of TRIM5α in any of the cell types tested. Mutation of the SIM consensus sequences, however, abolished TRIM5α antiviral activity against N-MLV. Mutation of lysines at a potential site of SUMOylation in the CA region of the Gag gene reduced the SUMO-1 block and the TRIM5α restriction of N-MLV. Our data suggest a novel aspect of TRIM5α-mediated restriction, in which the presence of intact SIMs in TRIM5α, and also the SUMO conjugation of CA, are required for restriction. We propose that at least a portion of the antiviral activity of TRIM5α is mediated through the binding of its SIMs to SUMO-conjugated CA.

TRIM56-mediated monoubiquitination of cGAS for cytosolic DNA sensing.

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Seo, Gil Ju; Kim, Charlotte; Shin, Woo-Jin; Sklan, Ella H; Eoh, Hyungjin; Jung, Jae U

2018-02-09

Intracellular nucleic acid sensors often undergo sophisticated modifications that are critical for the regulation of antimicrobial responses. Upon recognition of DNA, the cytosolic sensor cyclic GMP-AMP (cGAMP) synthase (cGAS) produces the second messenger cGAMP, which subsequently initiates downstream signaling to induce interferon-αβ (IFNαβ) production. Here we report that TRIM56 E3 ligase-induced monoubiquitination of cGAS is important for cytosolic DNA sensing and IFNαβ production to induce anti-DNA viral immunity. TRIM56 induces the Lys335 monoubiquitination of cGAS, resulting in a marked increase of its dimerization, DNA-binding activity, and cGAMP production. Consequently, TRIM56-deficient cells are defective in cGAS-mediated IFNαβ production upon herpes simplex virus-1 (HSV-1) infection. Furthermore, TRIM56-deficient mice show impaired IFNαβ production and high susceptibility to lethal HSV-1 infection but not to influenza A virus infection. This adds TRIM56 as a crucial component of the cytosolic DNA sensing pathway that induces anti-DNA viral innate immunity.

TRIM E3 ligases interfere with early and late stages of the retroviral life cycle.

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Pradeep D Uchil

2008-02-01

Full Text Available Members of the TRIpartite interaction Motif (TRIM family of E3 ligases have been shown to exhibit antiviral activities. Here we report a near comprehensive screen for antiretroviral activities of 55 TRIM proteins (36 human, 19 mouse. We identified approximately 20 TRIM proteins that, when transiently expressed in HEK293 cells, affect the entry or release of human immunodeficiency virus 1 (HIV, murine leukemia virus (MLV, or avian leukosis virus (ALV. While TRIM11 and 31 inhibited HIV entry, TRIM11 enhanced N-MLV entry by interfering with Ref1 restriction. Strikingly, many TRIM proteins affected late stages of the viral life cycle. Gene silencing of endogenously expressed TRIM 25, 31, and 62 inhibited viral release indicating that they play an important role at late stages of the viral life cycle. In contrast, downregulation of TRIM11 and 15 enhanced virus release suggesting that these proteins contribute to the endogenous restriction of retroviruses in cells.

Project INTEGRATE - a common methodological approach to understand integrated health care in Europe

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Lucinda Cash-Gibson

2014-12-01

Full Text Available Background: The use of case studies in health services research has proven to be an excellent methodology for gaining in-depth understanding of the organisation and delivery of health care. This is particularly relevant when looking at the complexity of integrated healthcare programmes, where multifaceted interactions occur at the different levels of care and often without a clear link between the interventions (new and/or existing and their impact on outcomes (in terms of patients health, both patient and professional satisfaction and cost-effectiveness. Still, integrated care is seen as a core strategy in the sustainability of health and care provision in most societies in Europe and beyond. More specifically, at present, there is neither clear evidence on transferable factors of integrated care success nor a method for determining how to establish these specific success factors. The drawback of case methodology in this case, however, is that the in-depth results or lessons generated are usually highly context-specific and thus brings the challenge of transferability of findings to other settings, as different health care systems and different indications are often not comparable. Project INTEGRATE, a European Commission-funded project, has been designed to overcome these problems; it looks into four chronic conditions in different European settings, under a common methodology framework (taking a mixed-methods approach to try to overcome the issue of context specificity and limited transferability. The common methodological framework described in this paper seeks to bring together the different case study findings in a way that key lessons may be derived and transferred between countries, contexts and patient-groups, where integrated care is delivered in order to provide insight into generalisability and build on existing evidence in this field.Methodology: To compare the different integrated care experiences, a mixed-methods approach has

Trim coil power supplies

International Nuclear Information System (INIS)

Haisler, R.; Peeler, H.; Zajicek, W.

1985-01-01

The 18 trim coil power supplies have been constructed and are now in place in the K500 pit and pit mezzanine. Final wiring of the primary power and control power is proceeding along with installation of cooling water supplies. The supplies are expected to be ready for final testing into resistive loads at the beginning of June, 1985

Integration of infrared thermography into various maintenance methodologies

Science.gov (United States)

Morgan, William T.

1993-04-01

Maintenance methodologies are in developmental stages throughout the world as global competitiveness drives all industries to improve operational efficiencies. Rapid progress in technical advancements has added an additional strain on maintenance organizations to progressively change. Accompanying needs for advanced training and documentation is the demand for utilization of various analytical instruments and quantitative methods. Infrared thermography is one of the primary elements of engineered approaches to maintenance. Current maintenance methodologies can be divided into six categories; Routine ('Breakdown'), Preventive, Predictive, Proactive, Reliability-Based, and Total Productive (TPM) maintenance. Each of these methodologies have distinctive approaches to achieving improved operational efficiencies. Popular though is that infrared thermography is a Predictive maintenance tool. While this is true, it is also true that it can be effectively integrated into each of the maintenance methodologies for achieving desired results. The six maintenance strategies will be defined. Infrared applications integrated into each will be composed in tabular form.

TRIM8 downregulation in glioma affects cell proliferation and it is associated with patients survival

International Nuclear Information System (INIS)

Micale, Lucia; Fusco, Carmela; Fontana, Andrea; Barbano, Raffaela; Augello, Bartolomeo; De Nittis, Pasquelena; Copetti, Massimiliano; Pellico, Maria Teresa; Mandriani, Barbara; Cocciadiferro, Dario; Parrella, Paola; Fazio, Vito Michele; Dimitri, Lucia Maria Cecilia; D’Angelo, Vincenzo; Novielli, Chiara; Larizza, Lidia; Daga, Antonio; Merla, Giuseppe

2015-01-01

Human gliomas are a heterogeneous group of primary malignant brain tumors whose molecular pathogenesis is not yet solved. In this regard, a major research effort has been directed at identifying novel specific glioma-associated genes. Here, we investigated the effect of TRIM8 gene in glioma. TRIM8 transcriptional level was profiled in our own glioma cases collection by qPCR and confirmed in the independent TCGA glioma cohort. The association between TRIM8 expression and Overall Survival and Progression-free Survival in TCGA cohort was determined by using uni-multivariable Cox regression analysis. The effect of TRIM8 on patient glioma cell proliferation was evaluated by performing MTT and clonogenic assays. The mechanisms causing the reduction of TRIM8 expression were explored by using qPCR and in vitro assays. We showed that TRIM8 expression correlates with unfavorable clinical outcome in glioma patients. We found that a restored TRIM8 expression induced a significant reduction of clonogenic potential in U87MG and patient’s glioblastoma cells. Finally we provide experimental evidences showing that miR-17 directly targets the 3′ UTR of TRIM8 and post-transcriptionally represses the expression of TRIM8. Our study provides evidences that TRIM8 may participate in the carcinogenesis and progression of glioma and that the transcriptional repression of TRIM8 might have potential value for predicting poor prognosis in glioma patients. The online version of this article (doi:10.1186/s12885-015-1449-9) contains supplementary material, which is available to authorized users

TRIM15 is a focal adhesion protein that regulates focal adhesion disassembly

Science.gov (United States)

Uchil, Pradeep D.; Pawliczek, Tobias; Reynolds, Tracy D.; Ding, Siyuan; Hinz, Angelika; Munro, James B.; Huang, Fang; Floyd, Robert W.; Yang, Haitao; Hamilton, William L.; Bewersdorf, Joerg; Xiong, Yong; Calderwood, David A.; Mothes, Walther

2014-01-01

ABSTRACT Focal adhesions are macromolecular complexes that connect the actin cytoskeleton to the extracellular matrix. Dynamic turnover of focal adhesions is crucial for cell migration. Paxillin is a multi-adaptor protein that plays an important role in regulating focal adhesion dynamics. Here, we identify TRIM15, a member of the tripartite motif protein family, as a paxillin-interacting factor and a component of focal adhesions. TRIM15 localizes to focal contacts in a myosin-II-independent manner by an interaction between its coiled-coil domain and the LD2 motif of paxillin. Unlike other focal adhesion proteins, TRIM15 is a stable focal adhesion component with restricted mobility due to its ability to form oligomers. TRIM15-depleted cells display impaired cell migration and reduced focal adhesion disassembly rates, in addition to enlarged focal adhesions. Thus, our studies demonstrate a cellular function for TRIM15 as a regulatory component of focal adhesion turnover and cell migration. PMID:25015296

Peak-Seeking Control for Trim Optimization

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National Aeronautics and Space Administration — Innovators have developed a peak-seeking algorithm that can reduce drag and improve performance and fuel efficiency by optimizing aircraft trim in real time. The...

AN INTEGRATED METHODOLOGY FOR CUSTOMER RELATIONSHIP MANAGEMENT CUSTOMIZATION

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Ricardo Colomo Palacios

2008-02-01

Full Text Available The importance and presence of technological solutions in organizations supporting CRM are a vital business fact from the late nineties. Presently, the manufacturers figure in the market has dramatically decreased because of continuous takeovers and merges, but it has on the other hand gained momentum because of the sudden open-source and on-demand solutions appearance. In this scope, a unified methodology centered on CRM solutions is of paramount importance since it has traditionally been linked to either system integration or overall solution design. Based on the two de-facto complementary standards for the implementation and development of Information Systems, namely the ESA and Dyché CRM systems implementation methodology, in this paper, we provide a CRM business solutions customization methodology which pertains independently to the integration and tool maker perspective.

Trimming and clustering sugarcane ESTs

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Guilherme P. Telles

2001-12-01

Full Text Available The original clustering procedure adopted in the Sugarcane Expressed Sequence Tag project (SUCEST had many problems, for instance too many clusters, the presence of ribosomal sequences, etc. We therefore redesigned the clustering procedure entirely, including a much more careful initial trimming of the reads. In this paper the new trimming and clustering strategies are described in detail and we give the new official figures for the project, 237,954 expressed sequence tags and 43,141 clusters.O método de clustering adotado no Projeto SUCEST (Sugarcane EST Project tinha vários problemas (muitos clusters, presença de seqüências de ribossomo etc. Nós assumimos a tarefa de reprojetar todo o processo de clustering, propondo uma "limpeza" inicial mais cuidadosa das seqüências. Neste artigo as estratégias de limpeza das seqüências e de clustering são descritas em detalhe, incluindo os números oficiais do projeto (237,954 ESTs e 43,141 clusters.

Potential role of TRIM3 as a novel tumour suppressor in colorectal cancer (CRC) development.

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Piao, Mei-Yu; Cao, Hai-Long; He, Na-Na; Xu, Meng-Que; Dong, Wen-Xiao; Wang, Wei-Qiang; Wang, Bang-Mao; Zhou, Bing

2016-01-01

Colorectal cancer (CRC) is the third leading cause of cancer-related mortality in the United States. Recent cancer genome-sequencing efforts and complementary functional studies have led to the identification of a collection of candidate 'driver' genes involved in CRC tumorigenesis. Tripartite motif (TRIM3) is recently identified as a tumour suppressor in glioblastoma but this tumour-suppressive function has not been investigated in CRC. In this study, we investigated the potential role of TRIM3 as a tumour suppressor in CRC development by manipulating the expression of TRIM3 in two authentic CRC cell lines, HCT116 and DLD1, followed by various functional assays, including cell proliferation, colony formation, scratch wound healing, soft agar, and invasion assays. Xenograft experiment was performed to examine in vivo tumour-suppressive properties of TRIM3. Small-interfering RNA (siRNA) mediated knockdown of TRIM3 conferred growth advantage in CRC cells. In contrast, overexpression of TRIM3 affected cell survival, cell migration, anchorage independent growth and invasive potential in CRC cells. In addition, TRIM3 was found to be down-regulated in human colon cancer tissues compared with matched normal colon tissues. Overexpression of TRIM3 significantly inhibited tumour growth in vivo using xenograft mouse models. Mechanistic investigation revealed that TRIM3 can regulate p53 protein level through its stabilisation. TRIM3 functions as a tumour suppressor in CRC progression. This tumour-suppressive function is exerted partially through regulation of p53 protein. Therefore, this protein may represent a novel therapeutic target for prevention or intervention of CRC.

Environmental impact assessment for energy pathways: an integrated methodology

International Nuclear Information System (INIS)

Sommereux-Blanc, Isabelle

2010-01-01

This document presents the synthesis of my research work contributing to the development of an integrated methodology of environmental impact assessment for energy pathways. In the context of world globalization, environmental impact assessments issues are highly linked with the following questioning: Which environmental impacts? for which demand? at which location? at which temporal scale? My work is built upon the definition of a conceptual framework able to handle these issues and upon its progressive implementation. The integration of the spatial and temporal issues within the methodology are key elements. Fundamental cornerstones of this framework are presented along the DPSIR concept (Driving forces, Pressures, State, Impacts, Responses). They cover a comprehensive analysis of the limits and the relevance of life cycle analysis and the development of a geo-spatialized environmental performance approach for an electrical production pathway. Perspectives linked with the development of this integrated methodology are detailed for energy pathways. (author)

Genetic evaluation of claw health traits accounting for potential preselection of cows to be trimmed.

Science.gov (United States)

Croué, Iola; Fikse, Freddy; Johansson, Kjell; Carlén, Emma; Thomas, Gilles; Leclerc, Hélène; Ducrocq, Vincent

2017-10-01

Claw lesions are one of the most important health issues in dairy cattle. Although the frequency of claw lesions depends greatly on herd management, the frequency can be lowered through genetic selection. A genetic evaluation could be developed based on trimming records collected by claw trimmers; however, not all cows present in a herd are usually selected by the breeder to be trimmed. The objectives of this study were to investigate the importance of the preselection of cows for trimming, to account for this preselection, and to estimate genetic parameters of claw health traits. The final data set contained 25,511 trimming records of French Holstein cows. Analyzed claw lesion traits were digital dermatitis, heel horn erosion, interdigital hyperplasia, sole hemorrhage circumscribed, sole hemorrhage diffused, sole ulcer, and white line fissure. All traits were analyzed as binary traits in a multitrait linear animal model. Three scenarios were considered: including only trimmed cows in a 7-trait model (scenario 1); or trimmed cows and contemporary cows not trimmed but present at the time of a visit (considering that nontrimmed cows were healthy) in a 7-trait model (scenario 2); or trimmed cows and contemporary cows not trimmed but present at the time of a visit (considering lesion records for trimmed cows only), in an 8-trait model, including a 0/1 trimming status trait (scenario 3). For scenario 3, heritability estimates ranged from 0.02 to 0.09 on the observed scale. Genetic correlations clearly revealed 2 groups of traits (digital dermatitis, heel horn erosion, and interdigital hyperplasia on the one hand, and sole hemorrhage circumscribed, sole hemorrhage diffused, sole ulcer, and white line fissure on the other hand). Heritabilities on the underlying scale did not vary much depending on the scenario: the effect of the preselection of cows for trimming on the estimation of heritabilities appeared to be negligible. However, including untrimmed cows as healthy

Structure and catalytic activation of the TRIM23 RING E3 ubiquitin ligase: DAWIDZIAK et al.

Energy Technology Data Exchange (ETDEWEB)

Dawidziak, Daria M. [Department of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville Virginia; Sanchez, Jacint G. [Department of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville Virginia; Wagner, Jonathan M. [Department of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville Virginia; Ganser-Pornillos, Barbie K. [Department of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville Virginia; Pornillos, Owen [Department of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville Virginia

2017-07-24

Tripartite motif (TRIM) proteins comprise a large family of RING-type ubiquitin E3 ligases that regulate important biological processes. An emerging general model is that TRIMs form elongated antiparallel coiled-coil dimers that prevent interaction of the two attendant RING domains. The RING domains themselves bind E2 conjugating enzymes as dimers, implying that an active TRIM ligase requires higher-order oligomerization of the basal coiled-coil dimers. Here, we report crystal structures of the TRIM23 RING domain in isolation and in complex with an E2–ubiquitin conjugate. Our results indicate that TRIM23 enzymatic activity requires RING dimerization, consistent with the general model of TRIM activation.

Nuclear TRIM25 Specifically Targets Influenza Virus Ribonucleoproteins to Block the Onset of RNA Chain Elongation.

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Meyerson, Nicholas R; Zhou, Ligang; Guo, Yusong R; Zhao, Chen; Tao, Yizhi J; Krug, Robert M; Sawyer, Sara L

2017-11-08

TRIM25 is an E3 ubiquitin ligase that activates RIG-I to promote the antiviral interferon response. The NS1 protein from all strains of influenza A virus binds TRIM25, although not all virus strains block the interferon response, suggesting alternative mechanisms for TRIM25 action. Here we present a nuclear role for TRIM25 in specifically restricting influenza A virus replication. TRIM25 inhibits viral RNA synthesis through a direct mechanism that is independent of its ubiquitin ligase activity and the interferon pathway. This activity can be inhibited by the viral NS1 protein. TRIM25 inhibition of viral RNA synthesis results from its binding to viral ribonucleoproteins (vRNPs), the structures containing individual viral RNA segments, the viral polymerase, and multiple viral nucleoproteins. TRIM25 binding does not inhibit initiation of capped-RNA-primed viral mRNA synthesis by the viral polymerase. Rather, the onset of RNA chain elongation is inhibited because TRIM25 prohibits the movement of RNA into the polymerase complex. Copyright © 2017 Elsevier Inc. All rights reserved.

Reliability evaluation methodologies for ensuring container integrity of stored transuranic (TRU) waste

International Nuclear Information System (INIS)

Smith, K.L.

1995-06-01

This report provides methodologies for providing defensible estimates of expected transuranic waste storage container lifetimes at the Radioactive Waste Management Complex. These methodologies can be used to estimate transuranic waste container reliability (for integrity and degradation) and as an analytical tool to optimize waste container integrity. Container packaging and storage configurations, which directly affect waste container integrity, are also addressed. The methodologies presented provide a means for demonstrating Resource Conservation and Recovery Act waste storage requirements

HIRFL-SSC trim coil currents calculation by conjugate gradients method

International Nuclear Information System (INIS)

Liu, W.

2005-01-01

For accelerating different kinds of ions to various energies, the HIRFL-SSC should form the corresponding isochronous magnetic field by its main coil and trim coils. Previously, there were errors in fitting the theoretical isochronous magnetic field in the small radius region, which led to some operation difficulties for ion acceleration in the inject region. After further investigation of the restrictive condition of the maximum current limitation, the trim coil currents for fitting the theoretical isochronous magnetic field were recalculated by the conjugate gradients method. Better results were obtained in the operation of HIRFL-SSC. This article introduces the procedure to calculate the trim coil currents. The calculation method of conjugate gradients is introduced and the fitting error is analysed. (author)

CFD-Based Over-Determined Trim Analysis for Optimum Aerodynamic Efficiency, Phase I

Data.gov (United States)

National Aeronautics and Space Administration — The overall objective of this Phase I project is to develop a nonlinear trim module in FUN3D for enabling the determined and over-determined trim analyses to be...

The effect of Trim5 polymorphisms on the clinical course of HIV-1 infection.

Directory of Open Access Journals (Sweden)

Daniëlle van Manen

2008-02-01

Full Text Available The antiviral factor tripartite interaction motif 5alpha (Trim5alpha restricts a broad range of retroviruses in a species-specific manner. Although human Trim5alpha is unable to block HIV-1 infection in human cells, a modest inhibition of HIV-1 replication has been reported. Recently two polymorphisms in the Trim5 gene (H43Y and R136Q were shown to affect the antiviral activity of Trim5alpha in vitro. In this study, participants of the Amsterdam Cohort studies were screened for polymorphisms at amino acid residue 43 and 136 of the Trim5 gene, and the potential effects of these polymorphisms on the clinical course of HIV-1 infection were analyzed. In agreement with the reported decreased antiviral activity of Trim5alpha that contains a Y at amino acid residue 43 in vitro, an accelerated disease progression was observed for individuals who were homozygous for the 43Y genotype as compared to individuals who were heterozygous or homozygous for the 43H genotype. A protective effect of the 136Q genotype was observed but only after the emergence of CXCR4-using (X4 HIV-1 variants and when a viral load of 10(4.5 copies per ml plasma was used as an endpoint in survival analysis. Interestingly, naive CD4 T cells, which are selectively targeted by X4 HIV-1, revealed a significantly higher expression of Trim5alpha than memory CD4 T cells. In addition, we observed that the 136Q allele in combination with the -2GG genotype in the 5'UTR was associated with an accelerated disease progression. Thus, polymorphisms in the Trim5 gene may influence the clinical course of HIV-1 infection also underscoring the antiviral effect of Trim5alpha on HIV-1 in vivo.

The Effect of Trim5 Polymorphisms on the Clinical Course of HIV-1 Infection

Science.gov (United States)

van Manen, Daniëlle; Rits, Maarten A. N; Beugeling, Corrine; van Dort, Karel; Schuitemaker, Hanneke; Kootstra, Neeltje A

2008-01-01

The antiviral factor tripartite interaction motif 5α (Trim5α) restricts a broad range of retroviruses in a species-specific manner. Although human Trim5α is unable to block HIV-1 infection in human cells, a modest inhibition of HIV-1 replication has been reported. Recently two polymorphisms in the Trim5 gene (H43Y and R136Q) were shown to affect the antiviral activity of Trim5α in vitro. In this study, participants of the Amsterdam Cohort studies were screened for polymorphisms at amino acid residue 43 and 136 of the Trim5 gene, and the potential effects of these polymorphisms on the clinical course of HIV-1 infection were analyzed. In agreement with the reported decreased antiviral activity of Trim5α that contains a Y at amino acid residue 43 in vitro, an accelerated disease progression was observed for individuals who were homozygous for the 43Y genotype as compared to individuals who were heterozygous or homozygous for the 43H genotype. A protective effect of the 136Q genotype was observed but only after the emergence of CXCR4-using (X4) HIV-1 variants and when a viral load of 104.5 copies per ml plasma was used as an endpoint in survival analysis. Interestingly, naive CD4 T cells, which are selectively targeted by X4 HIV-1, revealed a significantly higher expression of Trim5α than memory CD4 T cells. In addition, we observed that the 136Q allele in combination with the −2GG genotype in the 5′UTR was associated with an accelerated disease progression. Thus, polymorphisms in the Trim5 gene may influence the clinical course of HIV-1 infection also underscoring the antiviral effect of Trim5α on HIV-1 in vivo. PMID:18248091

Characterization and biological function analysis of the TRIM47 gene from common carp (Cyprinus carpio).

Science.gov (United States)

Wang, Yeda; Kuang, Ming; Lu, Yuanan; Lin, Li; Liu, Xueqin

2017-09-05

The TRIM family protein was known to play an important role in many cellular processes, including potential antiviral activity, which has attracted lots of attention. In this study, a TRIM47 homolog from common carp (Cyprinus carpio) was cloned and the full length coding DNA sequence (CDS) of this gene was analyzed, results showed that there was a 97% similarity between common carp and zebrafish (Danio rerio), but only 18% similarity with that of human (Homo sapiens) and mouse (Mus musculus). The tissue distribution analysis showed TRIM47 had the highest mRNA level in the brain, a few immune related organs such as liver and kidney also had a relatively high level of TRIM47 expression. SVCV infection decreased TRIM47 mRNA level significantly both in vitro and in vivo, but its expression was not affected by the virus at the protein level. The recombinant plasmid pcDNA4-TRIM47-His was constructed, the subcellular localization in FHM cells showed that TRIM47 uniformly distributed in the cytoplasm at the form of tiny spots, and partially localized in the mitochondria. Overexpression TRIM47 in FHM cells significantly decreased the mRNA level of SVCV-G gene, and it was accompanied with the increasing of IFN1, a member of type I IFN, at the case of SVCV stimulation. In summary, our results had first demonstrated that TRIM47 of the common carp played an important role in viral resistance processes as well as the regulation of IFN signaling pathway. Copyright © 2017. Published by Elsevier B.V.

Endoscopic removal and trimming of distal self-expandable metallic biliary stents

Science.gov (United States)

Ishii, Kentaro; Itoi, Takao; Sofuni, Atsushi; Itokawa, Fumihide; Tsuchiya, Takayoshi; Kurihara, Toshio; Tsuji, Shujiro; Ikeuchi, Nobuhito; Umeda, Junko; Moriyasu, Fuminori; Tsuchida, Akihiko

2011-01-01

AIM: To evaluate the efficacy and safety of endoscopic removal and trimming of self-expandable metallic stents (SEMS). METHODS: All SEMS had been placed for distal biliary strictures. Twenty-seven endoscopic procedures were performed in 19 patients in whom SEMS (one uncovered and 18 covered) removal had been attempted, and 8 patients in whom stent trimming using argon plasma coagulation (APC) had been attempted at Tokyo Medical University Hospital. The APC settings were: voltage 60-80 W and gas flow at 1.5 L/min. RESULTS: The mean stent indwelling period for all patients in whom stent removal had been attempted was 113.7 ± 77.6 d (range, 8-280 d). Of the 19 patients in whom removal of the SEMS had been attempted, the procedure was successful in 14 (73.7%) without procedure-related adverse events. The indwelling period in the stent removable group was shorter than that in the unremovable group (94.9 ± 71.5 d vs 166.2 ± 76.2 d, P = 0.08). Stent trimming was successful for all patients with one minor adverse event consisting of self-limited hemorrhage. Trimming time ranged from 11 to 16 min. CONCLUSION: Although further investigations on larger numbers of cases are necessary to accumulate evidence, the present data suggested that stent removal and stent trimming is feasible and effective for stent-related complications. PMID:21677835

S-nitrosylation of TRIM72 at cysteine 144 is critical for protection against oxidation-induced protein degradation and cell death.

Science.gov (United States)

Kohr, Mark J; Evangelista, Alicia M; Ferlito, Marcella; Steenbergen, Charles; Murphy, Elizabeth

2014-04-01

Oxidative stress and membrane damage following myocardial ischemia/reperfusion injury are important contributors to cardiomyocyte death and the loss of myocardial function. Our previous study identified cysteine 144 (C144) of tripartite motif-containing protein 72 (TRIM72) as a potential site for S-nitrosylation (SNO). TRIM72 is a cardioprotective membrane repair protein that can be both activated and targeted for degradation by different oxidative modifications. Consistent with the potential regulation of TRIM72 by various oxidative modifications, we found that SNO levels increased at C144 of TRIM72 with ischemic preconditioning. Therefore, to investigate the role of C144 in the regulation of TRIM72 function, we mutated C144 of TRIM72 to a serine residue (TRIM72(C144S)), and expressed either TRIM72(WT) or TRIM72(C144S) in HEK-293 cells, which lack endogenous TRIM72, in order to examine the effect of this mutation on the functional stability of TRIM72 and on cell survival. We hypothesized that SNO of TRIM72 stabilizes the protein, thus allowing for membrane repair and enhanced cell survival. Upon treatment with hydrogen peroxide (H2O2), we found that TRIM72(WT) levels were decreased, but not TRIM72(C144S) and this correlated with increased H2O2-induced cell death in TRIM72(WT) cells. Additionally, we found that treatment with the cardioprotective S-nitrosylating agent S-nitrosoglutathione (GSNO), was able to preserve TRIM72(WT) protein levels and enhance TRIM72(WT)-mediated cell survival, but had no effect on TRIM72(C144S) levels. Consistent with our hypothesis, GSNO was also found to increase SNO levels and inhibit H2O2-induced irreversible oxidation for TRIM72(WT) without affecting TRIM72(C144S). In further support of our hypothesis, GSNO blocked the ischemia/reperfusion-induced decrease in TRIM72 levels and reduced infarct size in a Langendorff-perfused heart model. The results of these studies have important implications for cardioprotection and suggest that

A 3D Hybrid Integration Methodology for Terabit Transceivers

DEFF Research Database (Denmark)

Dong, Yunfeng; Johansen, Tom Keinicke; Zhurbenko, Vitaliy

2015-01-01

integration are described. An equivalent circuit model of the via-throughs connecting the RF circuitry to the modulator is proposed and its lumped element parameters are extracted. Wire bonding transitions between the driving and RF circuitry were designed and simulated. An optimized 3D interposer design......This paper presents a three-dimensional (3D) hybrid integration methodology for terabit transceivers. The simulation methodology for multi-conductor structures are explained. The effect of ground vias on the RF circuitry and the preferred interposer substrate material for large bandwidth 3D hybrid...

Identification and characterization of a nuclear localization signal of TRIM28 that overlaps with the HP1 box

International Nuclear Information System (INIS)

Moriyama, Tetsuji; Sangel, Percival; Yamaguchi, Hiroki; Obuse, Chikashi; Miyamoto, Yoichi; Oka, Masahiro; Yoneda, Yoshihiro

2015-01-01

Tripartite motif-containing 28 (TRIM28) is a transcription regulator, which forms a repressor complex containing heterochromatin protein 1 (HP1). Here, we report identification of a nuclear localization signal (NLS) within the 462-494 amino acid region of TRIM28 that overlaps with its HP1 binding site, HP1 box. GST-pulldown experiments revealed the interaction of the arginine-rich TRIM28 NLS with various importin α subtypes (α1, α2 and α4). In vitro transport assay demonstrated that nuclear localization of GFP-TRIM28 NLS is mediated by importin αs, in conjunction with importin β1 and Ran. Further, we demonstrated that HP1 and importin αs compete for binding to TRIM28. Together, our findings suggest that importin α has an essential role in the nuclear delivery and preferential HP1 interaction of TRIM28. - Highlights: • TRIM28 contains an NLS within the 462-494 amino acid region. • The nuclear import of TRIM28 is mediated by importin α/importin β1. • TRIM28 NLS overlaps with HP1 Box. • HP1 and importin α compete for binding to TRIM28

Identification and characterization of a nuclear localization signal of TRIM28 that overlaps with the HP1 box

Energy Technology Data Exchange (ETDEWEB)

Moriyama, Tetsuji; Sangel, Percival [Laboratory of Nuclear Transport Dynamics, National Institutes of Biomedical Innovation, Health and Nutrition (NIBIOHN), Osaka 567-0085 (Japan); Yamaguchi, Hiroki [School of Medicine, Osaka University, Osaka 565-0871 (Japan); Obuse, Chikashi [Graduate School of Life Science, Hokkaido University, Sapporo 001-0021 (Japan); Miyamoto, Yoichi [Laboratory of Nuclear Transport Dynamics, National Institutes of Biomedical Innovation, Health and Nutrition (NIBIOHN), Osaka 567-0085 (Japan); Oka, Masahiro, E-mail: moka@nibiohn.go.jp [Laboratory of Nuclear Transport Dynamics, National Institutes of Biomedical Innovation, Health and Nutrition (NIBIOHN), Osaka 567-0085 (Japan); Laboratory of Biomedical Innovation, Graduate School of Pharmaceutical Sciences, Osaka University, Osaka 565-0871 (Japan); Yoneda, Yoshihiro, E-mail: y-yoneda@nibiohn.go.jp [National Institutes of Biomedical Innovation, Health and Nutrition (NIBIOHN), Osaka 567-0085 (Japan); Laboratory of Biomedical Innovation, Graduate School of Pharmaceutical Sciences, Osaka University, Osaka 565-0871 (Japan)

2015-07-03

Tripartite motif-containing 28 (TRIM28) is a transcription regulator, which forms a repressor complex containing heterochromatin protein 1 (HP1). Here, we report identification of a nuclear localization signal (NLS) within the 462-494 amino acid region of TRIM28 that overlaps with its HP1 binding site, HP1 box. GST-pulldown experiments revealed the interaction of the arginine-rich TRIM28 NLS with various importin α subtypes (α1, α2 and α4). In vitro transport assay demonstrated that nuclear localization of GFP-TRIM28 NLS is mediated by importin αs, in conjunction with importin β1 and Ran. Further, we demonstrated that HP1 and importin αs compete for binding to TRIM28. Together, our findings suggest that importin α has an essential role in the nuclear delivery and preferential HP1 interaction of TRIM28. - Highlights: • TRIM28 contains an NLS within the 462-494 amino acid region. • The nuclear import of TRIM28 is mediated by importin α/importin β1. • TRIM28 NLS overlaps with HP1 Box. • HP1 and importin α compete for binding to TRIM28.

Expression of TRIM28 correlates with proliferation and Bortezomib-induced apoptosis in B-cell non-Hodgkin lymphoma.

Science.gov (United States)

Zhang, Pei-Pei; Ding, Da-Zhi; Shi, Bing; Zhang, Shu-Qing; Gu, Ling-Li; Wang, Yu-Chan; Cheng, Chun

2018-03-23

Tripartite motif containing 28 (TRIM28) as a transcriptional co-repressor has been reported playing a role in regulating DNA damage response (DDR), cell differentiation, immune response, and tumorigenesis. The present study was performed to explore the biological function and clinical significance of TRIM28 in B-cell non-Hodgkin lymphoma (B-NHL). Results of the study displayed that high expression of TRIM28 was positively associated with the poorer survival of B-NHL patients as an independent prognostic factor. In addition, TRIM28 could promote the B-NHL cells proliferation through modulating cell cycle progression. The change of cyclinA, P21, and PCNA expression after TRIM28 expression modified further illustrated the mechanism in which TRIM28 participated in cell proliferation progression. Moreover, inhibition TRIM28 expression in B-NHL cells enhanced the sensibility to Bortezomib by regulating p53-mediated apoptosis pathway. Taken together, the present study showed that TRIM28 functions as a tumor promoter in B-NHL and may be a novel target for drug resistance to Bortezomib.

A quality control method by ultrasonic vibration energy and diagnosis system at trimming process

International Nuclear Information System (INIS)

Suh, Chang Min; Song, Gil Ho; Pyoun, Young Shik

2007-01-01

In this paper, the characteristics in mechanical properties of ultrasonic cold forging treatment (UCFT) used for the trimming knife and the effects of ultrasonic vibration energy (UVE) into the trimming process on the state of the strip cutting face were studied. And a diagnosis system to quality control for trimming knife and strip cutting face was developed and installed in plant. By the plant application of UCFT, service life of knife was more increased over 100% than that of conventional knife and using the developed diagnosis system, the knife breakage and saw ear have been perfectly detected and quality control of trimming face is effectively obtained

The ability of White Leghorn hens with trimmed comb and wattles to thermoregulate.

Science.gov (United States)

Al-Ramamneh, D S; Makagon, M M; Hester, P Y

2016-08-01

It is estimated that each year over 19 million pullets in the United States have their combs partially trimmed at a young age to improve egg production and feed efficiency. A possible disadvantage of trimming is that the comb and wattles may be essential for thermoregulation during hot weather allowing for conductive cooling of the blood through vasodilation of superficial vessels in these integumentary tissues. The objective of this study was to investigate the effects of partial comb and wattle removal, performed at 21 d of age, on the ability of White Leghorns to thermoregulate before, during, and after an imposed heating episode that averaged 34.6°C for 50.5 h. An increase in mortality at 20 h and body temperature at 48 h post initiation of the heating episode demonstrated that hens with trimmed comb and wattles were not able to cope with heat stress as effectively as controls. The increase in wattle temperature in controls as compared to trimmed hens during the heating episode and following heat stress provides supportive evidence that blood pools to the peripheral surface for conductive heat loss. During high temperatures typical of summer, trimmed hens attempted to compensate for their lack of ability to transfer heat from their comb and wattles to the environment through increased proportion of panting and wing spreading. Under less extreme conditions with lowered ambient temperatures, the trimming of the comb and wattles did not impair the ability of hens to thermoregulate, as body temperatures and behavior were similar to controls with no mortality. Egg weight was the only production parameter adversely affected by the trimming of the comb and wattles as compared to controls. The implication is that hens need their combs and wattles to thermoregulate effectively during periods of high environmental temperature. Pullets should not be subjected to a comb and wattle trim if they are housed in laying facilities that are not appropriately cooled during the

Matlab Stability and Control Toolbox: Trim and Static Stability Module

Science.gov (United States)

Crespo, Luis G.; Kenny, Sean P.

2006-01-01

This paper presents the technical background of the Trim and Static module of the Matlab Stability and Control Toolbox. This module performs a low-fidelity stability and control assessment of an aircraft model for a set of flight critical conditions. This is attained by determining if the control authority available for trim is sufficient and if the static stability characteristics are adequate. These conditions can be selected from a prescribed set or can be specified to meet particular requirements. The prescribed set of conditions includes horizontal flight, take-off rotation, landing flare, steady roll, steady turn and pull-up/ push-over flight, for which several operating conditions can be specified. A mathematical model was developed allowing for six-dimensional trim, adjustable inertial properties, asymmetric vehicle layouts, arbitrary number of engines, multi-axial thrust vectoring, engine(s)-out conditions, crosswind and gyroscopic effects.

The Timber Resource Inventory Model (TRIM): a projection model for timber supply and policy analysis.

Science.gov (United States)

P.L. Tedder; R.N. La Mont; J.C. Kincaid

1987-01-01

TRIM (Timber Resource Inventory Model) is a yield table projection system developed for timber supply projections and policy analysis. TRIM simulates timber growth, inventories, management and area changes, and removals over the projection period. Programs in the TRIM system, card-by-card descriptions of required inputs, table formats, and sample results are presented...

Networks as integrated in research methodologies in PER

DEFF Research Database (Denmark)

Bruun, Jesper

2016-01-01

of using networks to create insightful maps of learning discussions. To conclude, I argue that conceptual blending is a powerful framework for constructing "mixed methods" methodologies that may integrate diverse theories and other methodologies with network methodologies.......In recent years a number of researchers within the PER community have started using network analysis as a new methodology to extend our understanding of teaching and learning physics by viewing these as complex systems. In this paper, I give examples of social, cognitive, and action mapping...... networks and how they can be analyzed. In so doing I show how a network can be methodologically described as a set of relations between a set of entities, and how a network can be characterized and analyzed as a mathematical object. Then, as an illustrative example, I discuss a relatively new example...

An Integrated Safety Assessment Methodology for Generation IV Nuclear Systems

International Nuclear Information System (INIS)

Leahy, Timothy J.

2010-01-01

The Generation IV International Forum (GIF) Risk and Safety Working Group (RSWG) was created to develop an effective approach for the safety of Generation IV advanced nuclear energy systems. Early work of the RSWG focused on defining a safety philosophy founded on lessons learned from current and prior generations of nuclear technologies, and on identifying technology characteristics that may help achieve Generation IV safety goals. More recent RSWG work has focused on the definition of an integrated safety assessment methodology for evaluating the safety of Generation IV systems. The methodology, tentatively called ISAM, is an integrated 'toolkit' consisting of analytical techniques that are available and matched to appropriate stages of Generation IV system concept development. The integrated methodology is intended to yield safety-related insights that help actively drive the evolving design throughout the technology development cycle, potentially resulting in enhanced safety, reduced costs, and shortened development time.

Synchronization Of TRIMs Principles In The Legislation Of Mining Sector In Indonesia

Directory of Open Access Journals (Sweden)

Agus Lanini

2015-08-01

Full Text Available The aims of this research are to know and explain synchronizing the principles of TRIMs at the foreign direct investment FDI and mining regulation in Indonesia. The research using normative research that conducted through library or document study such legal resources as primary sources secondary and tertiary sources that will be studied with applied legal approach comparative history and conceptual approach. Research results are revealing that synchronizing between the principles of TRIMs and Investment act 2007and Mineral and Coal act 2009 were not harmonized. It should be TRIMs and investment act 2007 have some similarity principles as harmonized even though Mineral and Coal act 2009 does not enough accessibility for the principles of TRIMs. Implication of the Mineral and Coal act 2009 raise a strong and real authority of the state even central or local government. Those policy results an overlap regulation caused state management system over natural resources ineffective.

TRIM29 Overexpression Promotes Proliferation and Survival of Bladder Cancer Cells through NF-κB Signaling.

Science.gov (United States)

Tan, Shu-Tao; Liu, Sheng-Ye; Wu, Bin

2016-10-01

TRIM29 overexpression has been reported in several human malignancies and showed correlation with cancer cell malignancy. The aim of the current study is to examine its clinical significance and biological roles in human bladder cancer tissues and cell lines. A total of 102 cases of bladder cancer tissues were examined for TRIM29 expression by immunohistochemistry. siRNA and plasmid transfection were performed in 5637 and BIU-87 cell lines. Cell Counting Kit-8, flow cytometry, western blot, and real-time polymerase chain reaction were performed to examine its biological roles and mechanism in bladder cancer cells. We found that TRIM29 overexpression showed correlation with invading depth (p=0.0087). Knockdown of TRIM29 expression in bladder cancer cell line 5637 inhibited cell growth rate and cell cycle transition while its overexpression in BIU-87 cells accelerated cell proliferation and cell cycle progression. TRIM29 overexpression also inhibited cell apoptosis induced by cisplatin. In addition, we demonstrated that TRIM29 depletion decreased while its overexpression led to upregulated expression of cyclin D1, cyclin E, and Bcl-2. We also showed that TRIM29 knockdown inhibited protein kinase C (PKC) and nuclear factor κB (NF-κB) signaling while its overexpression stimulated the PKC and NF-κB pathways. BAY 11-7082 (NF-κB inhibitor) partly attenuated the effect of TRIM29 on expression of cyclin and Bcl-2. Treatment with PKC inhibitor staurosporine resulted in ameliorated TRIM29 induced activation of NF-κB. The current study demonstrated that TRIM29 upregulates cyclin and Bcl family proteins level to facilitate malignant cell growth and inhibit drug-induced apoptosis in bladder cancer, possibly through PKC-NF-κB signaling pathways.

TRIM32 promotes retinoic acid receptor α-mediated differentiation in human promyelogenous leukemic cell line HL60

International Nuclear Information System (INIS)

Sato, Tomonobu; Okumura, Fumihiko; Iguchi, Akihiro; Ariga, Tadashi; Hatakeyama, Shigetsugu

2012-01-01

Highlights: ► TRIM32 enhanced RARα-mediated transcriptional activity even in the absence of RA. ► TRIM32 stabilized RARα in the human promyelogenous leukemic cell line HL60. ► Overexpression of TRIM32 in HL60 cells induced granulocytic differentiation. ► TRIM32 may function as a coactivator for RARα-mediated transcription in APL cells. -- Abstract: Ubiquitination, one of the posttranslational modifications, appears to be involved in the transcriptional activity of nuclear receptors including retinoic acid receptor α (RARα). We previously reported that an E3 ubiquitin ligase, TRIM32, interacts with several important proteins including RARα and enhances transcriptional activity of RARα in mouse neuroblastoma cells and embryonal carcinoma cells. Retinoic acid (RA), which acts as a ligand to nuclear receptors including RARα, plays crucial roles in development, differentiation, cell cycles and apoptosis. In this study, we found that TRIM32 enhances RARα-mediated transcriptional activity even in the absence of RA and stabilizes RARα in the human promyelogenous leukemic cell line HL60. Moreover, we found that overexpression of TRIM32 in HL60 cells suppresses cellular proliferation and induces granulocytic differentiation even in the absence of RA. These findings suggest that TRIM32 functions as one of the coactivators for RARα-mediated transcription in acute promyelogenous leukemia (APL) cells, and thus TRIM32 may become a potentially therapeutic target for APL.

Investigation of an automatic trim algorithm for restructurable aircraft control

Science.gov (United States)

Weiss, J.; Eterno, J.; Grunberg, D.; Looze, D.; Ostroff, A.

1986-01-01

This paper develops and solves an automatic trim problem for restructurable aircraft control. The trim solution is applied as a feed-forward control to reject measurable disturbances following control element failures. Disturbance rejection and command following performances are recovered through the automatic feedback control redesign procedure described by Looze et al. (1985). For this project the existence of a failure detection mechanism is assumed, and methods to cope with potential detection and identification inaccuracies are addressed.

Development of the SSC [Superconducting Super Collider] trim coil beam tube assembly

International Nuclear Information System (INIS)

Skaritka, J.; Kelly, E.; Schneider, W.

1987-01-01

The Superconducting Super Collider uses ≅9600 dipole magnets. The magnets have been carefully designed to exhibit minimal magnetic field harmonics. However, because of superconductor magnetization effects, iron saturation and conductor/coil positioning errors, certain harmonic errors are possible and must be corrected by use of multipole correctors called trim coils. For the most efficient use of axial space in the magnet, and lowest possible current, a distributed internal correction coil design is planned. The trim coil assembly is secured to the beam tube, a uhv tube with special strength, size, conductivity and vacuum. The report details the SSC trim coil/beam tube assembly specifications, history, and ongoing development

TRIM24 promotes glioma progression and enhances chemoresistance through activation of the PI3K/Akt signaling pathway.

Science.gov (United States)

Zhang, L-H; Yin, A-A; Cheng, J-X; Huang, H-Y; Li, X-M; Zhang, Y-Q; Han, N; Zhang, X

2015-01-29

The tripartite motif protein TRIM24 (tripartite motif-containing 24) has been found to play distinct roles in tumor development and progression, according to different tumor contexts. However, it remains elusive whether TRIM24 plays a role in malignant gliomas that are the most common and deadly primary brain tumors in adults. We report here that TRIM24 expression is positively correlated with glioma malignancy and is negatively associated with prognosis of patients with newly diagnosed glioblastoma, which is the most malignant form of gliomas but displays highly heterogeneous clinical outcome. The multivariate Cox regression analysis demonstrates the independent predictive value of TRIM24 expression level for overall and progression-free survival. Knockdown of TRIM24 suppresses cell proliferation, cell cycle progression, clone formation and in vivo tumor development, whereas overexpression of TRIM24 promotes cell growth. Chromatin immunoprecipitation, real-time reverse transcription-PCR and mutation analyses demonstrate that TRIM24 binds to the PIK3CA promoter via its PHD-Bromo domain to activate the transcription of PIK3CA gene, thus enhancing phosphatidylinositide 3-kinase (PI3K)/Akt signaling. The pan-PI3K inhibitor LY294002 and small interfering RNA targeting PIK3CA both abrogate the growth-promoting effect of TRIM24. Moreover, TRIM24 regulates the expression of DNA repair enzyme O(6)-methylguanine-DNA methyltransferase (MGMT) through PI3K/Akt/nuclear factor-κB signaling transduction and enhances resistance to temozolomide, the standard chemotherapeutic agent for glioblastoma. Finally, glioblastoma patients with low TRIM24 expression benefit from chemotherapy, whereas those with high TRIM24 expression do not have such benefit. Our results suggest that TRIM24 might serve as a potential prognostic marker and therapeutic target for the management of malignant gliomas.

An Integrated Methodology for Emulsified Formulated Product Design

DEFF Research Database (Denmark)

Mattei, Michele

are mixed together to determine the desired emulsified product. They are still mainly designed and analysed through trial - and - error based exper- imental techniques, therefore a systematic approach , integrating model-based as well a s experiment - based techniques, for design of these products could......The consumer oriented chemical based products are used every day by millions of people. They are structured products constituted of numerous chemicals, and many of them, especially household and personal care products, are emulsions where active ingredients, solvents, additives and surfactants...... significantly reduce both time and cost connected to product development by doing only the necessary experi- ments , and ensuring chances for innovation . The main contribution of this project i s the development of an integrated methodology for the design of emulsified formulated products. The methodology...

Development and Operation of an Automatic Rotor Trim Control System for the UH-60 Individual Blade Control Wind Tunnel Test

Science.gov (United States)

Theodore, Colin R.; Tischler, Mark B.

2010-01-01

An automatic rotor trim control system was developed and successfully used during a wind tunnel test of a full-scale UH-60 rotor system with Individual Blade Control (IBC) actuators. The trim control system allowed rotor trim to be set more quickly, precisely and repeatably than in previous wind tunnel tests. This control system also allowed the rotor trim state to be maintained during transients and drift in wind tunnel flow, and through changes in IBC actuation. The ability to maintain a consistent rotor trim state was key to quickly and accurately evaluating the effect of IBC on rotor performance, vibration, noise and loads. This paper presents details of the design and implementation of the trim control system including the rotor system hardware, trim control requirements, and trim control hardware and software implementation. Results are presented showing the effect of IBC on rotor trim and dynamic response, a validation of the rotor dynamic simulation used to calculate the initial control gains and tuning of the control system, and the overall performance of the trim control system during the wind tunnel test.

TRIM44 Is a Poor Prognostic Factor for Breast Cancer Patients as a Modulator of NF-κB Signaling.

Science.gov (United States)

Kawabata, Hidetaka; Azuma, Kotaro; Ikeda, Kazuhiro; Sugitani, Ikuko; Kinowaki, Keiichi; Fujii, Takeshi; Osaki, Akihiko; Saeki, Toshiaki; Horie-Inoue, Kuniko; Inoue, Satoshi

2017-09-08

Many of the tripartite motif (TRIM) proteins function as E3 ubiquitin ligases and are assumed to be involved in various events, including oncogenesis. In regard to tripartite motif-containing 44 (TRIM44), which is an atypical TRIM family protein lacking the RING finger domain, its pathophysiological significance in breast cancer remains unknown. We performed an immunohistochemical study of TRIM44 protein in clinical breast cancer tissues from 129 patients. The pathophysiological role of TRIM44 in breast cancer was assessed by modulating TRIM44 expression in MCF-7 and MDA-MB-231 breast cancer cells. TRIM44 strong immunoreactivity was significantly associated with nuclear grade ( p = 0.033), distant disease-free survival ( p = 0.031) and overall survival ( p = 0.027). Multivariate analysis revealed that the TRIM44 status was an independent prognostic factor for distant disease-free survival ( p = 0.005) and overall survival ( p = 0.002) of patients. siRNA-mediated TRIM44 knockdown significantly decreased the proliferation of MCF-7 and MDA-MB-231 cells and inhibited the migration of MDA-MB-231 cells. Microarray analysis and qRT-PCR showed that TRIM44 knockdown upregulated CDK19 and downregulated MMP1 in MDA-MB-231 cells. Notably, TRIM44 knockdown impaired nuclear factor-kappa B (NF-κB)-mediated transcriptional activity stimulated by tumor necrosis factor α (TNFα). Moreover, TRIM44 knockdown substantially attenuated the TNFα-dependent phosphorylation of the p65 subunit of NF-κB and IκBα in both MCF-7 and MDA-MB-231 cells. TRIM44 would play a role in the progression of breast cancer by promoting cell proliferation and migration, as well as by enhancing NF-κB signaling.

Over-expression of TRIM37 promotes cell migration and metastasis in hepatocellular carcinoma by activating Wnt/β-catenin signaling

Energy Technology Data Exchange (ETDEWEB)

Jiang, Jianxin; Yu, Chao; Chen, Meiyuan; Tian, She; Sun, Chengyi, E-mail: chenyisun11@163.com

2015-09-04

Hepatocellular carcinoma (HCC) is the most common cancer in the world especially in East Asia and Africa. Advanced stage, metastasis and frequent relapse are responsible for the poor prognosis of HCC. However, the precise mechanisms underlying HCC remained unclear. So it is urgent to identify the pathological processes and relevant molecules of HCC. TRIM37 is an E3 ligase and has been observed deregulated expression in various tumors. Recent studies of TRIM37 have implicated that TRIM37 played critical roles in cell proliferation and other processes. In the present study, we demonstrated that TRIM37 expression was notably up-regulated in HCC samples and was associated with advanced stage and tumor volume, which all indicating the poor outcomes. We also found that TRIM37 could serve as an independent prognostic factor of HCC. During the course of in vitro and in vivo work, we showed that TRIM37 promoted HCC cells migration and metastasis by inducing EMT. Furthermore, we revealed that the effect of TRIM37 mediated EMT in HCC cells was achieved by the activation of Wnt/β-catenin signaling. These finding may provide insight into the understanding of TRIM37 as a novel critical factor of HCC and a candidate target for HCC treatment. - Highlights: • Highly expression of TRIM37 is found in HCC samples compared with nontumorous samples. • TRIM37 expression is correlated with advanced HCC stages and could be an independent prognostic factor. • TRIM37 promotes cell proliferation and metastasis. • We report an E3 ligase TRIM37 affects Wnt/β-catenin signaling.

Molecular Characterization, Tissue Distribution and Expression, and Potential Antiviral Effects of TRIM32 in the Common Carp (Cyprinus carpio)

Science.gov (United States)

Wang, Yeda; Li, Zeming; Lu, Yuanan; Hu, Guangfu; Lin, Li; Zeng, Lingbing; Zhou, Yong; Liu, Xueqin

2016-01-01

Tripartite motif-containing protein 32 (TRIM32) belongs to the tripartite motif (TRIM) family, which consists of a large number of proteins containing a RING (Really Interesting New Gene) domain, one or two B-box domains, and coiled coil motif followed by different C-terminal domains. The TRIM family is known to be implicated in multiple cellular functions, including antiviral activity. However, it is presently unknown whether TRIM32 of common carp (Cyprinus carpio) has the antiviral effect. In this study, the sequence, expression, and antiviral function of TRIM32 homolog from common carp were analyzed. The full-length coding sequence region of trim32 was cloned from common carp. The results showed that the expression of TRIM32 (mRNA) was highest in the brain, remained stably expressed during embryonic development, and significantly increased following spring viraemia of carp virus (SVCV) infection. Transient overexpression of TRIM32 in affected Epithelioma papulosum cyprinid cells led to significant decrease of SVCV production as compared to the control group. These results suggested a potentially important role of common carp TRIM32 in enhancing host immune response during SVCV infection both in vivo and in vitro. PMID:27735853

Identification of TRIM27 as a novel degradation target of herpes simplex virus 1 ICP0.

Science.gov (United States)

Conwell, Sara E; White, Anne E; Harper, J Wade; Knipe, David M

2015-01-01

The herpes simplex virus 1 (HSV-1) immediate early protein ICP0 performs many functions during infection, including transactivation of viral gene expression, suppression of innate immune responses, and modification and eviction of histones from viral chromatin. Although these functions of ICP0 have been characterized, the detailed mechanisms underlying ICP0's complex role during infection warrant further investigation. We thus undertook an unbiased proteomic approach to identifying viral and cellular proteins that interact with ICP0 in the infected cell. Cellular candidates resulting from our analysis included the ubiquitin-specific protease USP7, the transcriptional repressor TRIM27, DNA repair proteins NBN and MRE11A, regulators of apoptosis, including BIRC6, and the proteasome. We also identified two HSV-1 early proteins involved in nucleotide metabolism, UL39 and UL50, as novel candidate interactors of ICP0. Because TRIM27 was the most statistically significant cellular candidate, we investigated the relationship between TRIM27 and ICP0. We observed rapid, ICP0-dependent loss of TRIM27 during HSV-1 infection. TRIM27 protein levels were restored by disrupting the RING domain of ICP0 or by inhibiting the proteasome, arguing that TRIM27 is a novel degradation target of ICP0. A mutant ICP0 lacking E3 ligase activity interacted with endogenous TRIM27 during infection as demonstrated by reciprocal coimmunoprecipitation and supported by immunofluorescence data. Surprisingly, ICP0-null mutant virus yields decreased upon TRIM27 depletion, arguing that TRIM27 has a positive effect on infection despite being targeted for degradation. These results illustrate a complex interaction between TRIM27 and viral infection with potential positive or negative effects of TRIM27 on HSV under different infection conditions. During productive infection, a virus must simultaneously redirect multiple cellular pathways to replicate itself while evading detection by the host's defenses. To

Effect of Hardwood Sawmill Edging and Trimming Practices on Furniture Part Production

Science.gov (United States)

D. Earl Kline; Carmen Regalado; Eugene M. Wengert; Fred M. Lamb; Philip A. Araman

1993-01-01

In a recent edging and trimming study at three hardwood sawmills, it was observed that the lumber volume produced was approximately 10 percent less than would be necessary to make the most valuable lumber. Furthermore, the excess portion of wood that was removed from the edging and trimming process contained a large percentage of clear wood. In light of rising costs...

TRIM32 ubiquitin E3 ligase, one enzyme for several pathologies: From muscular dystrophy to tumours.

Science.gov (United States)

Lazzari, Elisa; Meroni, Germana

2016-10-01

TRIM32 is a member of the TRIpartite Motif family characterised by the presence of an N-terminal three-domain-module that includes a RING domain, which confers E3 ubiquitin ligase activity, one or two B-box domains and a Coiled-Coil region that mediates oligomerisation. Several TRIM32 substrates were identified including muscular proteins and proteins involved in cell cycle regulation and cell motility. As ubiquitination is a versatile post-translational modification that can affect target turnover, sub-cellular localisation or activity, it is likely that diverse substrates may be differentially affected by TRIM32-mediated ubiquitination, reflecting its multi-faceted roles in muscle physiology, cancer and immunity. With particular relevance for muscle physiology, mutations in TRIM32 are associated with autosomal recessive Limb-Girdle Muscular Dystrophy 2H, a muscle-wasting disease with variable clinical spectrum ranging from almost asymptomatic to wheelchair-bound patients. In this review, we will focus on the ability of TRIM32 to mark specific substrates for proteasomal degradation discussing how the TRIM32-proteasome axis may (i) be important for muscle homeostasis and for the pathogenesis of muscular dystrophy; and (ii) define either an oncogenic or tumour suppressive role for TRIM32 in the context of different types of cancer. Copyright © 2016 Elsevier Ltd. All rights reserved.

Moving characteristics of single file passengers considering the effect of ship trim and heeling

Science.gov (United States)

Sun, Jinlu; Lu, Shouxiang; Lo, Siuming; Ma, Jian; Xie, Qimiao

2018-01-01

Ship listing and motion affects the movement pattern of passengers on board, thus pedestrian traffic and evacuation dynamics would be significantly different from those on level ground. To quantify the influence of ship listing and motion on passenger evacuation, we designed a ship corridor simulator, with which we performed single-file pedestrian movement experiments considering the effect of trim and heeling. Results indicated that density is not the only factor that affects pedestrian speed under ship trim or heeling conditions, for that both individual walking speed and group walking speed would be greatly attenuated due to the influence of the trim angles. However, heeling angles show less impact on speed when compared with trim angles. In addition, the speed correlation coefficient between the adjacent experimental subjects would be higher with larger angles and lower speed. Moreover, both female and male experimental subjects need similar distance headway for walking in different trim or heeling conditions. Furthermore, experimental subjects with lower individual walking speed need longer time headway to keep enough distance headway. This work will provide fundamental guidance to the development of evacuation models and the design of evacuation facilities on board.

76 FR 72978 - Premier Trim, LLC, Spectrum Trim, LLC and Grant Products International, Inc. D/B/A Spectrum Grant...

Science.gov (United States)

2011-11-28

..., Spectrum Trim, LLC and Grant Products International, Inc. D/B/A Spectrum Grant De Mexico Including Workers Whose Unemployment Insurance (UI) Wages Are Paid Through Grant Products International, Inc... Brownsville, TX; Amended Certification Regarding Eligibility To Apply for Worker Adjustment Assistance In...

77 FR 67399 - Trim Systems Operating Corp., a Subsidiary of Commercial Vehicle Group, Inc., Including On-Site...

Science.gov (United States)

2012-11-09

... DEPARTMENT OF LABOR Employment and Training Administration [TA-W-81,393] Trim Systems Operating..., applicable to workers and former workers of Trim Systems Operating Corp., a subsidiary of Commercial Vehicle.... The amended notice applicable to TA-W-81,393 is hereby issued as follows: All workers of Trim Systems...

Molecular characterization of a CpTRIM35-like protein and its splice variants from whitespotted bamboo shark (Chiloscyllium plagiosum)

Energy Technology Data Exchange (ETDEWEB)

Zhang, Xinshang, E-mail: sanmaosound@163.com; Zhao, Heng, E-mail: hengzhao2000@gmail.com; Chen, Yeyu, E-mail: cyyleaf@126.com; Luo, Huiying, E-mail: luohuiying@caas.cn; Yao, Bin, E-mail: binyao@caas.cn

2014-10-24

Highlights: • A TRIM gene and three splice variants were firstly cloned from elasmobranch fish. • The genes were constitutively expressed with high levels in spleen and kidney. • The gene products were distributed in cytoplasm alone or cytoplasm and nucleus. • As E3 ubiquitin ligases, the proteins differed in immune responses to challenges. - Abstract: The tripartite motif (TRIM) proteins play important roles in a broad range of biological processes, including apoptosis, cell proliferation and innate immunity response. In this study, a TRIM gene and its three splice variants were cloned from an elasmobranch fish—whitespotted bamboo shark (Chiloscyllium plagiosum Bennett). Phylogenetic analysis indicated that the gene was closely related to TRIM35 homologs, thus termed CpTRIM35-like. Deduced CpTRIM35 has a RBCC-PRY/SPRY structure typical of TRIM proteins, and its splice variants (CpTRIM35-1–3) have different truncations at the C-terminus. The gene products were constitutively expressed in adult sharks with the highest levels in spleen and kidney. The different subcellular locations, upregulation upon LPS and poly I:C stimulation, and significant E3 ubiquitin ligase activities suggested their different roles in immune responses as an E3 ubiquitin ligase. This is the first TRIM protein ever characterized in elasmobranch fish.

Molecular characterization of a CpTRIM35-like protein and its splice variants from whitespotted bamboo shark (Chiloscyllium plagiosum)

International Nuclear Information System (INIS)

Zhang, Xinshang; Zhao, Heng; Chen, Yeyu; Luo, Huiying; Yao, Bin

2014-01-01

Highlights: • A TRIM gene and three splice variants were firstly cloned from elasmobranch fish. • The genes were constitutively expressed with high levels in spleen and kidney. • The gene products were distributed in cytoplasm alone or cytoplasm and nucleus. • As E3 ubiquitin ligases, the proteins differed in immune responses to challenges. - Abstract: The tripartite motif (TRIM) proteins play important roles in a broad range of biological processes, including apoptosis, cell proliferation and innate immunity response. In this study, a TRIM gene and its three splice variants were cloned from an elasmobranch fish—whitespotted bamboo shark (Chiloscyllium plagiosum Bennett). Phylogenetic analysis indicated that the gene was closely related to TRIM35 homologs, thus termed CpTRIM35-like. Deduced CpTRIM35 has a RBCC-PRY/SPRY structure typical of TRIM proteins, and its splice variants (CpTRIM35-1–3) have different truncations at the C-terminus. The gene products were constitutively expressed in adult sharks with the highest levels in spleen and kidney. The different subcellular locations, upregulation upon LPS and poly I:C stimulation, and significant E3 ubiquitin ligase activities suggested their different roles in immune responses as an E3 ubiquitin ligase. This is the first TRIM protein ever characterized in elasmobranch fish

Integrated cost estimation methodology to support high-performance building design

Energy Technology Data Exchange (ETDEWEB)

Vaidya, Prasad; Greden, Lara; Eijadi, David; McDougall, Tom [The Weidt Group, Minnetonka (United States); Cole, Ray [Axiom Engineers, Monterey (United States)

2007-07-01

Design teams evaluating the performance of energy conservation measures (ECMs) calculate energy savings rigorously with established modelling protocols, accounting for the interaction between various measures. However, incremental cost calculations do not have a similar rigor. Often there is no recognition of cost reductions with integrated design, nor is there assessment of cost interactions amongst measures. This lack of rigor feeds the notion that high-performance buildings cost more, creating a barrier for design teams pursuing aggressive high-performance outcomes. This study proposes an alternative integrated methodology to arrive at a lower perceived incremental cost for improved energy performance. The methodology is based on the use of energy simulations as means towards integrated design and cost estimation. Various points along the spectrum of integration are identified and characterized by the amount of design effort invested, the scheduling of effort, and relative energy performance of the resultant design. It includes a study of the interactions between building system parameters as they relate to capital costs. Several cost interactions amongst energy measures are found to be significant.The value of this approach is demonstrated with alternatives in a case study that shows the differences between perceived costs for energy measures along various points on the integration spectrum. These alternatives show design tradeoffs and identify how decisions would have been different with a standard costing approach. Areas of further research to make the methodology more robust are identified. Policy measures to encourage the integrated approach and reduce the barriers towards improved energy performance are discussed.

Genome-wide association study for claw disorders and trimming status in dairy cattle

NARCIS (Netherlands)

Spek, van der D.; Arendonk, van J.A.M.; Bovenhuis, H.

2015-01-01

Performing a genome-wide association study (GWAS) might add to a better understanding of the development of claw disorders and the need for trimming. Therefore, the aim of the current study was to perform a GWAS on claw disorders and trimming status and to validate the results for claw disorders

TRIMS: Validating T2 Molecular Effects for Neutrino Mass Experiments

Science.gov (United States)

Lin, Ying-Ting; Trims Collaboration

2017-09-01

The Tritium Recoil-Ion Mass Spectrometer (TRIMS) experiment examines the branching ratio of the molecular tritium (T2) beta decay to the bound state (3HeT+). Measuring this branching ratio helps to validate the current molecular final-state theory applied in neutrino mass experiments such as KATRIN and Project 8. TRIMS consists of a magnet-guided time-of-flight mass spectrometer with a detector located on each end. By measuring the kinetic energy and time-of-flight difference of the ions and beta particles reaching the detectors, we will be able to distinguish molecular ions from atomic ones and hence derive the ratio in question. We will give an update on the apparatus, simulation software, and analysis tools, including efforts to improve the resolution of our detectors and to characterize the stability and uniformity of our field sources. We will also share our commissioning results and prospects for physics data. The TRIMS experiment is supported by U.S. Department of Energy Office of Science, Office of Nuclear Physics, Award Number DE-FG02-97ER41020.

Qualitative case study methodology in nursing research: an integrative review.

Science.gov (United States)

Anthony, Susan; Jack, Susan

2009-06-01

This paper is a report of an integrative review conducted to critically analyse the contemporary use of qualitative case study methodology in nursing research. Increasing complexity in health care and increasing use of case study in nursing research support the need for current examination of this methodology. In 2007, a search for case study research (published 2005-2007) indexed in the CINAHL, MEDLINE, EMBASE, PsychINFO, Sociological Abstracts and SCOPUS databases was conducted. A sample of 42 case study research papers met the inclusion criteria. Whittemore and Knafl's integrative review method guided the analysis. Confusion exists about the name, nature and use of case study. This methodology, including terminology and concepts, is often invisible in qualitative study titles and abstracts. Case study is an exclusive methodology and an adjunct to exploring particular aspects of phenomena under investigation in larger or mixed-methods studies. A high quality of case study exists in nursing research. Judicious selection and diligent application of literature review methods promote the development of nursing science. Case study is becoming entrenched in the nursing research lexicon as a well-accepted methodology for studying phenomena in health and social care, and its growing use warrants continued appraisal to promote nursing knowledge development. Attention to all case study elements, process and publication is important in promoting authenticity, methodological quality and visibility.

Automatic channel trimming for control systems: A concept

Science.gov (United States)

Vandervoort, R. J.; Sykes, H. A.

1977-01-01

Set of bias signals added to channel inputs automatically normalize differences between channels. Algorithm and second feedback loop compute trim biases. Concept could be applied to regulators and multichannel servosystems for remote manipulators in undersea mining.

Application of a Broadband Active Vibration Control System to a Helicopter Trim Panel

Science.gov (United States)

Cabell, Randolph H.; Schiller, Noah H.; Simon, Frank

2013-01-01

This paper discusses testing of a broadband active vibration control concept on an interior trim panel in a helicopter cabin mockup located at ONERA's Centre de Toulouse. The control system consisted of twelve diamond-shaped piezoelectric actuators distributed around a 1.2m x 1.2m trim panel. Accelerometers were mounted at the four vertices of each diamond. The aspect ratio of the diamond was based on the dielectric constants of the piezoelectric material in order to create an actuator-sensor pair that was collocated over a broad frequency range. This allowed robust control to be implemented using simple, low power analog electronics. Initial testing on a thick acrylic window demonstrated the capability of the controller, but actuator performance was less satisfactory when mounted on a composite sandwich trim panel. This may have been due to the orthotropic nature of the trim panel, or due to its much higher stiffness relative to the acrylic window. Insights gained from a finite element study of the actuator-sensor-structural system are discussed.

Study of Sylvilagus rabbit TRIM5α species-specific domain: how ancient endoviruses could have shaped the antiviral repertoire in Lagomorpha

Directory of Open Access Journals (Sweden)

Areal Helena

2011-10-01

Full Text Available Abstract Background Since the first report of the antiretroviral restriction factor TRIM5α in primates, several orthologs in other mammals have been described. Recent studies suggest that leporid retroviruses like RELIK, the first reported endogenous lentivirus ever, may have imposed positive selection in TRIM5α orthologs of the European rabbit and European brown hare. Considering that RELIK must already have been present in a common ancestor of the leporid genera Lepus, Sylvilagus and Oryctolagus, we extended the study of evolutionary patterns of TRIM5α to other members of the Leporidae family, particularly to the genus Sylvilagus. Therefore, we obtained the TRIM5α nucleotide sequences of additional subspecies and species of the three leporid genera. We also compared lagomorph TRIM5α deduced protein sequences and established TRIM5α gene and TRIM5α protein phylogenies. Results The deduced protein sequence of Iberian hare TRIM5α was 89% identical to European rabbit TRIM5α, although high divergence was observed at the PRYSPRY v1 region between rabbit and the identified alleles from this hare species (allele 1: 50% divergence; allele 2: 53% divergence. A high identity was expected between the Sylvilagus and Oryctolagus TRIM5α proteins and, in fact, the Sylvilagus TRIM5α was 91% identical to the Oryctolagus protein. Nevertheless, the PRYSPRY v1 region was only 50% similar between these genera. Selection analysis of Lagomorpha TRIM5α proteins identified 25 positively-selected codons, 11 of which are located in the PRYSPRY v1 region, responsible for species specific differences in viral capsid recognition. Conclusions By extending Lagomorpha TRIM5α studies to an additional genus known to bear RELIK, we verified that the divergent species-specific pattern observed between the Oryctolagus and Lepus PRYSPRY-domains is also present in Sylvilagus TRIM5α. This work is one of the first known studies that compare the evolution of the

Methodology for dimensional variation analysis of ITER integrated systems

International Nuclear Information System (INIS)

Fuentes, F. Javier; Trouvé, Vincent; Cordier, Jean-Jacques; Reich, Jens

2016-01-01

Highlights: • Tokamak dimensional management methodology, based on 3D variation analysis, is presented. • Dimensional Variation Model implementation workflow is described. • Methodology phases are described in detail. The application of this methodology to the tolerance analysis of ITER Vacuum Vessel is presented. • Dimensional studies are a valuable tool for the assessment of Tokamak PCR (Project Change Requests), DR (Deviation Requests) and NCR (Non-Conformance Reports). - Abstract: The ITER machine consists of a large number of complex systems highly integrated, with critical functional requirements and reduced design clearances to minimize the impact in cost and performances. Tolerances and assembly accuracies in critical areas could have a serious impact in the final performances, compromising the machine assembly and plasma operation. The management of tolerances allocated to part manufacture and assembly processes, as well as the control of potential deviations and early mitigation of non-compliances with the technical requirements, is a critical activity on the project life cycle. A 3D tolerance simulation analysis of ITER Tokamak machine has been developed based on 3DCS dedicated software. This integrated dimensional variation model is representative of Tokamak manufacturing functional tolerances and assembly processes, predicting accurate values for the amount of variation on critical areas. This paper describes the detailed methodology to implement and update the Tokamak Dimensional Variation Model. The model is managed at system level. The methodology phases are illustrated by its application to the Vacuum Vessel (VV), considering the status of maturity of VV dimensional variation model. The following topics are described in this paper: • Model description and constraints. • Model implementation workflow. • Management of input and output data. • Statistical analysis and risk assessment. The management of the integration studies based on

Methodology for dimensional variation analysis of ITER integrated systems

Energy Technology Data Exchange (ETDEWEB)

Fuentes, F. Javier, E-mail: FranciscoJavier.Fuentes@iter.org [ITER Organization, Route de Vinon-sur-Verdon—CS 90046, 13067 St Paul-lez-Durance (France); Trouvé, Vincent [Assystem Engineering & Operation Services, rue J-M Jacquard CS 60117, 84120 Pertuis (France); Cordier, Jean-Jacques; Reich, Jens [ITER Organization, Route de Vinon-sur-Verdon—CS 90046, 13067 St Paul-lez-Durance (France)

2016-11-01

Highlights: • Tokamak dimensional management methodology, based on 3D variation analysis, is presented. • Dimensional Variation Model implementation workflow is described. • Methodology phases are described in detail. The application of this methodology to the tolerance analysis of ITER Vacuum Vessel is presented. • Dimensional studies are a valuable tool for the assessment of Tokamak PCR (Project Change Requests), DR (Deviation Requests) and NCR (Non-Conformance Reports). - Abstract: The ITER machine consists of a large number of complex systems highly integrated, with critical functional requirements and reduced design clearances to minimize the impact in cost and performances. Tolerances and assembly accuracies in critical areas could have a serious impact in the final performances, compromising the machine assembly and plasma operation. The management of tolerances allocated to part manufacture and assembly processes, as well as the control of potential deviations and early mitigation of non-compliances with the technical requirements, is a critical activity on the project life cycle. A 3D tolerance simulation analysis of ITER Tokamak machine has been developed based on 3DCS dedicated software. This integrated dimensional variation model is representative of Tokamak manufacturing functional tolerances and assembly processes, predicting accurate values for the amount of variation on critical areas. This paper describes the detailed methodology to implement and update the Tokamak Dimensional Variation Model. The model is managed at system level. The methodology phases are illustrated by its application to the Vacuum Vessel (VV), considering the status of maturity of VV dimensional variation model. The following topics are described in this paper: • Model description and constraints. • Model implementation workflow. • Management of input and output data. • Statistical analysis and risk assessment. The management of the integration studies based on

The Transcriptional Repressive Activity of KRAB Zinc Finger Proteins Does Not Correlate with Their Ability to Recruit TRIM28.

Directory of Open Access Journals (Sweden)

Kristin E Murphy

Full Text Available KRAB domain Zinc finger proteins are one of the most abundant families of transcriptional regulators in higher vertebrates. The prevailing view is that KRAB domain proteins function as potent transcriptional repressors by recruiting TRIM28 and promoting heterochromatin spreading. However, the extent to which all KRAB domain proteins are TRIM28-dependent transcriptional repressors is currently unclear. Our studies on mouse ZFP568 revealed that TRIM28 recruitment by KRAB domain proteins is not sufficient to warrant transcriptional repressive activity. By using luciferase reporter assays and yeast two-hybrid experiments, we tested the ability of ZFP568 and other mouse KRAB domain proteins to repress transcription and bind TRIM28. We found that some mouse KRAB domain proteins are poor transcriptional repressors despite their ability to recruit TRIM28, while others showed strong KRAB-dependent transcriptional repression, but no TRIM28 binding. Together, our results show that the transcriptional repressive activity of KRAB-ZNF proteins does not correlate with their ability to recruit TRIM28, and provide evidence that KRAB domains can regulate transcription in a TRIM28-independent fashion. Our findings challenge the current understanding of the molecular mechanisms used by KRAB domain proteins to control gene expression and highlight that a high percentage of KRAB domain proteins in the mouse genome differ from the consensus KRAB sequence at amino acid residues that are critical for TRIM28 binding and/or repressive activity.

Innovation and Integrity in Intervention Research: Conceptual Issues, Methodology, and Knowledge Translation.

Science.gov (United States)

Malti, Tina; Beelmann, Andreas; Noam, Gil G; Sommer, Simon

2018-04-01

In this article, we introduce the special issue entitled Innovation and Integrity in Intervention Science. Its focus is on essential problems and prospects for intervention research examining two related topics, i.e., methodological issues and research integrity, and challenges in the transfer of research knowledge into practice and policy. The main aims are to identify how to advance methodology in order to improve research quality, examine scientific integrity in the field of intervention science, and discuss future steps to enhance the transfer of knowledge about evidence-based intervention principles into sustained practice, routine activities, and policy decisions. Themes of the special issue are twofold. The first includes questions about research methodology in intervention science, both in terms of research design and methods, as well as data analyses and the reporting of findings. Second, the issue tackles questions surrounding the types of knowledge translation frameworks that might be beneficial to mobilize the transfer of research-based knowledge into practice and public policies. The issue argues that innovations in methodology and thoughtful approaches to knowledge translation can enable transparency, quality, and sustainability of intervention research.

Robust estimators based on generalization of trimmed mean

Czech Academy of Sciences Publication Activity Database

Adam, Lukáš; Bejda, P.

(2018) ISSN 0361-0918 Institutional support: RVO:67985556 Keywords : Breakdown point * Estimators * Geometric median * Location * Trimmed mean Subject RIV: BA - General Mathematics Impact factor: 0.457, year: 2016 http://library.utia.cas.cz/separaty/2017/MTR/adam-0481224.pdf

Methodological Problems on the Way to Integrative Human Neuroscience.

Science.gov (United States)

Kotchoubey, Boris; Tretter, Felix; Braun, Hans A; Buchheim, Thomas; Draguhn, Andreas; Fuchs, Thomas; Hasler, Felix; Hastedt, Heiner; Hinterberger, Thilo; Northoff, Georg; Rentschler, Ingo; Schleim, Stephan; Sellmaier, Stephan; Tebartz Van Elst, Ludger; Tschacher, Wolfgang

2016-01-01

Neuroscience is a multidisciplinary effort to understand the structures and functions of the brain and brain-mind relations. This effort results in an increasing amount of data, generated by sophisticated technologies. However, these data enhance our descriptive knowledge , rather than improve our understanding of brain functions. This is caused by methodological gaps both within and between subdisciplines constituting neuroscience, and the atomistic approach that limits the study of macro- and mesoscopic issues. Whole-brain measurement technologies do not resolve these issues, but rather aggravate them by the complexity problem. The present article is devoted to methodological and epistemic problems that obstruct the development of human neuroscience. We neither discuss ontological questions (e.g., the nature of the mind) nor review data, except when it is necessary to demonstrate a methodological issue. As regards intradisciplinary methodological problems, we concentrate on those within neurobiology (e.g., the gap between electrical and chemical approaches to neurophysiological processes) and psychology (missing theoretical concepts). As regards interdisciplinary problems, we suggest that core disciplines of neuroscience can be integrated using systemic concepts that also entail human-environment relations. We emphasize the necessity of a meta-discussion that should entail a closer cooperation with philosophy as a discipline of systematic reflection. The atomistic reduction should be complemented by the explicit consideration of the embodiedness of the brain and the embeddedness of humans. The discussion is aimed at the development of an explicit methodology of integrative human neuroscience , which will not only link different fields and levels, but also help in understanding clinical phenomena.

Methodological Problems on the Way to Integrative Human Neuroscience

Science.gov (United States)

Kotchoubey, Boris; Tretter, Felix; Braun, Hans A.; Buchheim, Thomas; Draguhn, Andreas; Fuchs, Thomas; Hasler, Felix; Hastedt, Heiner; Hinterberger, Thilo; Northoff, Georg; Rentschler, Ingo; Schleim, Stephan; Sellmaier, Stephan; Tebartz Van Elst, Ludger; Tschacher, Wolfgang

2016-01-01

Neuroscience is a multidisciplinary effort to understand the structures and functions of the brain and brain-mind relations. This effort results in an increasing amount of data, generated by sophisticated technologies. However, these data enhance our descriptive knowledge, rather than improve our understanding of brain functions. This is caused by methodological gaps both within and between subdisciplines constituting neuroscience, and the atomistic approach that limits the study of macro- and mesoscopic issues. Whole-brain measurement technologies do not resolve these issues, but rather aggravate them by the complexity problem. The present article is devoted to methodological and epistemic problems that obstruct the development of human neuroscience. We neither discuss ontological questions (e.g., the nature of the mind) nor review data, except when it is necessary to demonstrate a methodological issue. As regards intradisciplinary methodological problems, we concentrate on those within neurobiology (e.g., the gap between electrical and chemical approaches to neurophysiological processes) and psychology (missing theoretical concepts). As regards interdisciplinary problems, we suggest that core disciplines of neuroscience can be integrated using systemic concepts that also entail human-environment relations. We emphasize the necessity of a meta-discussion that should entail a closer cooperation with philosophy as a discipline of systematic reflection. The atomistic reduction should be complemented by the explicit consideration of the embodiedness of the brain and the embeddedness of humans. The discussion is aimed at the development of an explicit methodology of integrative human neuroscience, which will not only link different fields and levels, but also help in understanding clinical phenomena. PMID:27965548

Integrated structure/control design - Present methodology and future opportunities

Science.gov (United States)

Weisshaar, T. A.; Newsom, J. R.; Zeiler, T. A.; Gilbert, M. G.

1986-01-01

Attention is given to current methodology applied to the integration of the optimal design process for structures and controls. Multilevel linear decomposition techniques proved to be most effective in organizing the computational efforts necessary for ISCD (integrated structures and control design) tasks. With the development of large orbiting space structures and actively controlled, high performance aircraft, there will be more situations in which this concept can be applied.

GO-FLOW methodology. Basic concept and integrated analysis framework for its applications

International Nuclear Information System (INIS)

Matsuoka, Takeshi

2010-01-01

GO-FLOW methodology is a success oriented system analysis technique, and is capable of evaluating a large system with complex operational sequences. Recently an integrated analysis framework of the GO-FLOW has been developed for the safety evaluation of elevator systems by the Ministry of Land, Infrastructure, Transport and Tourism, Japanese Government. This paper describes (a) an Overview of the GO-FLOW methodology, (b) Procedure of treating a phased mission problem, (c) Common cause failure analysis, (d) Uncertainty analysis, and (e) Integrated analysis framework. The GO-FLOW methodology is a valuable and useful tool for system reliability analysis and has a wide range of applications. (author)

Assessment of gamma irradiation heating and damage in miniature neutron source reactor vessel using computational methods and SRIM - TRIM code

International Nuclear Information System (INIS)

Appiah-Ofori, F. F.

2014-07-01

its location within the vessel. The Numerical Algorithms were simulated using MATLAB Code developed for solution by Gaussian Elimination method. The methodology also employed SRIM - TRIM Code, to determine the number of defects produced by a primary knock-on atom (pka). In the SRIM - TRIM code, Detailed Calculation with Full Damage Cascades option was selected as the damage type with Tables 3.1 and 3.2 as the Ion and Target Data and input parameters. Cobalt ion of energy 5000 keV was directed to the target atom, AI, to interact with the lattice atoms (500) which produced 14052 vacancies and 14783 interstitials as microscopic defects. The vessel absorbed energy of 1.09 MeV and lattice atoms of the vessel (AI) were shifted or displaced from their site by 0.0207 times. DPA is a measure of amount of radiation damage in a solid induced by gamma irradiation. The Analytical and Numerical (CVFDM) solutions obtained from the models developed were far below the value of the melting point 933.47 K while the result obtained from the thermal stresses were also far below the yield stress 480 MPa (properties of the vessel) indicated that the induced maximum effective thermal stress due to the absorption of gamma ray of energy 5.0 MeV originating from the reactor core had relatively no effect or can posed no danger on the structural integrity of GHARR-I reactor vessel during operation. (au)

Development and Operation of an Automatic Rotor Trim Control System for use During the UH-60 Individual Blade Control Wind Tunnel Test

Science.gov (United States)

Theodore, Colin R.

2010-01-01

A full-scale wind tunnel test to evaluate the effects of Individual Blade Control (IBC) on the performance, vibration, noise and loads of a UH-60A rotor was recently completed in the National Full-Scale Aerodynamics Complex (NFAC) 40- by 80-Foot Wind Tunnel [1]. A key component of this wind tunnel test was an automatic rotor trim control system that allowed the rotor trim state to be set more precisely, quickly and repeatably than was possible with the rotor operator setting the trim condition manually. The trim control system was also able to maintain the desired trim condition through changes in IBC actuation both in open- and closed-loop IBC modes, and through long-period transients in wind tunnel flow. This ability of the trim control system to automatically set and maintain a steady rotor trim enabled the effects of different IBC inputs to be compared at common trim conditions and to perform these tests quickly without requiring the rotor operator to re-trim the rotor. The trim control system described in this paper was developed specifically for use during the IBC wind tunnel test

Trauma Risk Management (TRiM): Promoting Help Seeking for Mental Health Problems Among Combat-Exposed U.K. Military Personnel.

Science.gov (United States)

Jones, Norman; Burdett, Howard; Green, Kevin; Greenberg, Neil

2017-01-01

Trauma Risk Management (TRiM) is a peer-led, occupational mental health support process that aims to identify and assist U.K. military personnel with persistent mental ill health related to potentially traumatic events (PTEs). This study compared help seeking, mental disorder symptoms, and alcohol use between TRiM recipients and personnel experiencing similar combat events who did not receive TRiM; an unexposed group provided context. Records of TRiM activity during a U.K. military deployment in Afghanistan were linked to contemporaneous survey data assessing mental health and combat experiences. The resulting deployment data set was amalgamated with mental health, alcohol use, and help-seeking data collected within 12 weeks of homecoming and again one to two years later. Mental health and help-seeking outcomes were compared between a nonexposed, non-TRiM sample (n = 161), an exposed, non-TRiM sample (n = 149), and an exposed, TRiM-recipient sample (n = 328) using logistic regression analyses. At follow-up, TRiM recipients were significantly more likely to seek help from mental health services than exposed, non-TRiM personnel. At baseline, TRiM recipients had significantly greater adjusted odds of reporting possible posttraumatic stress disorder (PTSD) symptoms than exposed non-TRiM personnel; the difference was not significant at follow-up. TRiM recipients were significantly more likely to report persistent mental disorder and alcohol misuse caseness over the follow-up period. TRiM recipients were significantly more likely to seek help from mental health services than a similar PTE-exposed group that did not receive TRiM; however, TRiM recipients experienced more persistent mental ill-health symptoms and hazardous alcohol use over the period of follow-up despite seeking help.

A Comparison of Various Software Development Methodologies: Feasibility and Methods of Integration

Directory of Open Access Journals (Sweden)

Samir Abou El-Seoud

2016-12-01

Full Text Available System development methodologies which have being used in the academic and commercial environments during last two decades have advantages and disadvantages. Researchers had tried to identify objectives, scope …etc. of the methodologies by following different approaches. Each approach has its Limitation, specific interest, coverage …etc. In this paper, we tried to perform a comparative study of those methodologies which are popular and commonly used in banking and commercial environment. We tried in our study to determine objectives, scope, tools and other features of the methodologies. We also, tried to determine how and to what extent the methodologies incorporate the facilities such as project management, cost benefit analysis, documentation …etc. One of the most important aspects of our study was how to integrate the methodologies and develop a global methodology which covers the complete span of the software development life cycle? A prototype system which integrates the selected methodologies has been developed. The developed system helps analysts and designers how to choose suitable tools or to obtain guidelines on what to do in a particular situation. The prototype system has been tested during the development of a software for an ATM “Auto Teller Machine” by selecting and applying SASD methodology during software development. This resulted in the development of high quality and well documented software system.

Automatic Edging and Trimming of Hardwood Lumber

Science.gov (United States)

D. Earl Kline; Eugene M. Wengert; Philip A. Araman

1990-01-01

Studies have shown that there is a potential to increase hardwood lumber value by more than 20 percent through optimum edging and trimming. Even a small portion of this percentage can boost the profitability of hardwood lumber manufacturers substantially. The objective of this research project is to develop an automated system which would assist in correct edging and...

Trim9 Deletion Alters the Morphogenesis of Developing and Adult-Born Hippocampal Neurons and Impairs Spatial Learning and Memory.

Science.gov (United States)

Winkle, Cortney C; Olsen, Reid H J; Kim, Hyojin; Moy, Sheryl S; Song, Juan; Gupton, Stephanie L

2016-05-04

During hippocampal development, newly born neurons migrate to appropriate destinations, extend axons, and ramify dendritic arbors to establish functional circuitry. These developmental stages are recapitulated in the dentate gyrus of the adult hippocampus, where neurons are continuously generated and subsequently incorporate into existing, local circuitry. Here we demonstrate that the E3 ubiquitin ligase TRIM9 regulates these developmental stages in embryonic and adult-born mouse hippocampal neurons in vitro and in vivo Embryonic hippocampal and adult-born dentate granule neurons lacking Trim9 exhibit several morphological defects, including excessive dendritic arborization. Although gross anatomy of the hippocampus was not detectably altered by Trim9 deletion, a significant number of Trim9(-/-) adult-born dentate neurons localized inappropriately. These morphological and localization defects of hippocampal neurons in Trim9(-/-) mice were associated with extreme deficits in spatial learning and memory, suggesting that TRIM9-directed neuronal morphogenesis may be involved in hippocampal-dependent behaviors. Appropriate generation and incorporation of adult-born neurons in the dentate gyrus are critical for spatial learning and memory and other hippocampal functions. Here we identify the brain-enriched E3 ubiquitin ligase TRIM9 as a novel regulator of embryonic and adult hippocampal neuron shape acquisition and hippocampal-dependent behaviors. Genetic deletion of Trim9 elevated dendritic arborization of hippocampal neurons in vitro and in vivo Adult-born dentate granule cells lacking Trim9 similarly exhibited excessive dendritic arborization and mislocalization of cell bodies in vivo These cellular defects were associated with severe deficits in spatial learning and memory. Copyright © 2016 the authors 0270-6474/16/364940-19$15.00/0.

A Methodology for Conducting Integrative Mixed Methods Research and Data Analyses

Science.gov (United States)

Castro, Felipe González; Kellison, Joshua G.; Boyd, Stephen J.; Kopak, Albert

2011-01-01

Mixed methods research has gained visibility within the last few years, although limitations persist regarding the scientific caliber of certain mixed methods research designs and methods. The need exists for rigorous mixed methods designs that integrate various data analytic procedures for a seamless transfer of evidence across qualitative and quantitative modalities. Such designs can offer the strength of confirmatory results drawn from quantitative multivariate analyses, along with “deep structure” explanatory descriptions as drawn from qualitative analyses. This article presents evidence generated from over a decade of pilot research in developing an integrative mixed methods methodology. It presents a conceptual framework and methodological and data analytic procedures for conducting mixed methods research studies, and it also presents illustrative examples from the authors' ongoing integrative mixed methods research studies. PMID:22167325

Effect of two trimming methods of dairy cattle on concrete or rubber-covered slatted floors

NARCIS (Netherlands)

Ouweltjes, W.; Holzhauer, M.; Tol, van der P.P.J.; Werf, van der J.T.N.

2009-01-01

This study monitored claw health, claw conformation, locomotion, activity, and step traits of cows from a single dairy herd that were trimmed according to the standard Dutch method or with an alternative "concave" trimming method. Half of the cows were kept in a stall section with concrete slatted

An optimization methodology for identifying robust process integration investments under uncertainty

Energy Technology Data Exchange (ETDEWEB)

Svensson, Elin; Berntsson, Thore [Department of Energy and Environment, Division of Heat and Power Technology, Chalmers University of Technology, SE-412 96 Goeteborg (Sweden); Stroemberg, Ann-Brith [Fraunhofer-Chalmers Research Centre for Industrial Mathematics, Chalmers Science Park, SE-412 88 Gothenburg (Sweden); Patriksson, Michael [Department of Mathematical Sciences, Chalmers University of Technology and Department of Mathematical Sciences, University of Gothenburg, SE-412 96 Goeteborg (Sweden)

2009-02-15

Uncertainties in future energy prices and policies strongly affect decisions on investments in process integration measures in industry. In this paper, we present a five-step methodology for the identification of robust investment alternatives incorporating explicitly such uncertainties in the optimization model. Methods for optimization under uncertainty (or, stochastic programming) are thus combined with a deep understanding of process integration and process technology in order to achieve a framework for decision-making concerning the investment planning of process integration measures under uncertainty. The proposed methodology enables the optimization of investments in energy efficiency with respect to their net present value or an environmental objective. In particular, as a result of the optimization approach, complex investment alternatives, allowing for combinations of energy efficiency measures, can be analyzed. Uncertainties as well as time-dependent parameters, such as energy prices and policies, are modelled using a scenario-based approach, enabling the identification of robust investment solutions. The methodology is primarily an aid for decision-makers in industry, but it will also provide insight for policy-makers into how uncertainties regarding future price levels and policy instruments affect the decisions on investments in energy efficiency measures. (author)

An optimization methodology for identifying robust process integration investments under uncertainty

International Nuclear Information System (INIS)

Svensson, Elin; Berntsson, Thore; Stroemberg, Ann-Brith; Patriksson, Michael

2009-01-01

Uncertainties in future energy prices and policies strongly affect decisions on investments in process integration measures in industry. In this paper, we present a five-step methodology for the identification of robust investment alternatives incorporating explicitly such uncertainties in the optimization model. Methods for optimization under uncertainty (or, stochastic programming) are thus combined with a deep understanding of process integration and process technology in order to achieve a framework for decision-making concerning the investment planning of process integration measures under uncertainty. The proposed methodology enables the optimization of investments in energy efficiency with respect to their net present value or an environmental objective. In particular, as a result of the optimization approach, complex investment alternatives, allowing for combinations of energy efficiency measures, can be analyzed. Uncertainties as well as time-dependent parameters, such as energy prices and policies, are modelled using a scenario-based approach, enabling the identification of robust investment solutions. The methodology is primarily an aid for decision-makers in industry, but it will also provide insight for policy-makers into how uncertainties regarding future price levels and policy instruments affect the decisions on investments in energy efficiency measures. (author)

[Tripartite motif-containing protein 34 (TRIM34) colocalized with micronuclei chromosome and hampers its movement to equatorial plate during the metaphase stage of mitosis].

Science.gov (United States)

Sun, Dakang; An, Xinye; Ji, Bing; Cheng, Yanli; Gao, Honglian; Tian, Mingming

2016-06-01

Objective To examine whether tripartite motif-containing protein 34 (TRIM34) is colocalized with micronuclei and investigate the influence on the movement of micronuclei chromosome in mitosis. Methods The eukaryotic expression vector TRIM34-pEGFP-N3 was constructed, identified and then transfected into HEK293T cells. With 4', 6-diamidino-2-phenylindole 2HCI (DAPI) staining, the colocalization between TRIM34 and micronuclei was observed under a fluorescence microscope. Moreover, MitoTracker(R)Deep Red was used to identify the colocalization between the complex of TRIM34-micronulei and mitochondria under a confocal microscope. Finally, the effect of TRIM34 on the movement of micronuclei chromosome in mitosis was examined. Results DNA sequencing confirmed that the vector TRIM34-pEGFP-N3 was constructed successfully. A fluorescence microscope revealed that TRIM34 could be colocalized with micronuclei in HEK293T cells transfected with TRIM34-pEGFP-N3. In the same manner, a confocal microscope distinctly showed that TRIM34 was colocalized with micronuclei similarly in appearance. However, there was no distinguished colocalization relationship between the complex of TRIM34-micronulei and mitochondria. Interestingly, the micronuclei chromosome conjugated with TRIM34 was hardly transferred to equatorial plate during the metaphase stage of mitosis. Conclusion TRIM34 is colocalized with micronuclei chromosome and hampers its movement to equatorial plate in mitosis.

Integrated Management System - Scope, Possibilities And Methodology

Science.gov (United States)

Čekanová, Katarína

2015-06-01

Organizations are becoming more aware of the importance of integrated management systems (IMS). Interest in this subject indicates that IMS are seen as "management systems of the future". Based on this, the aim of this articles characterizes the possibility of building IMS through the identification of common elements and specific requirements in accordance with the ISO 9001, ISO 14001 and OHSAS 18001 professional references. Part of the article is the methodology of building IMS in the organization.

Influence of an accurate trimming operation on springback

NARCIS (Netherlands)

Avetisyan, Marianna; Meinders, Vincent T.; Huetink, Han

2006-01-01

When a sheet metal forming operation, such as deep drawing, is followed by a trimming operation the numerical springback is influenced by various physical parameters and numerical factors. Two techniques of taking into the account the stress state change due to cutting in the FE plate model are

The E3 Ubiquitin Ligase TRIM40 Attenuates Antiviral Immune Responses by Targeting MDA5 and RIG-I

Directory of Open Access Journals (Sweden)

Chunyuan Zhao

2017-11-01

Full Text Available Retinoic acid-inducible gene-I (RIG-I-like receptors (RLRs, including melanoma differentiation-associated gene 5 (MDA5 and RIG-I, are crucial for host recognition of non-self RNAs, especially viral RNA. Thus, the expression and activation of RLRs play fundamental roles in eliminating the invading RNA viruses and maintaining immune homeostasis. However, how RLR expression is tightly regulated remains to be further investigated. In this study, we identified a major histocompatibility complex (MHC-encoded gene, tripartite interaction motif 40 (TRIM40, as a suppressor of RLR signaling by directly targeting MDA5 and RIG-I. TRIM40 binds to MDA5 and RIG-I and promotes their K27- and K48-linked polyubiquitination via its E3 ligase activity, leading to their proteasomal degradation. TRIM40 deficiency enhances RLR-triggered signaling. Consequently, TRIM40 deficiency greatly enhances antiviral immune responses and decreases viral replication in vivo. Thus, we demonstrate that TRIM40 limits RLR-triggered innate activation, suggesting TRIM40 as a potential therapeutic target for the control of viral infection.

Methodology and integrated studies for the strategic regional uranium prospects

International Nuclear Information System (INIS)

Andrade Tolentino, J. de.

1984-01-01

An integrated methodology alternative to the traditional one used for uranium prospecting is presented. For this purpose a detailed revision of some methods for finding uranium was made. Then a geological unit located in Minas Gerais, Brazil, was chosen due to its geographical proximity to the Lagoa Real deposit in the Brazilian Bahia State. The methodology applied to this unit gave a preliminary indications of areas to be prospected in detail. 36 refs, 44 figs, 20 tabs

33 CFR 401.30 - Ballast water and trim.

Science.gov (United States)

2010-07-01

... 33 Navigation and Navigable Waters 3 2010-07-01 2010-07-01 false Ballast water and trim. 401.30... OF TRANSPORTATION SEAWAY REGULATIONS AND RULES Regulations Seaway Navigation § 401.30 Ballast water... exclusive economic zone must agree to comply with the “Code of Best Practices for Ballast Water Management...

tclust: An R Package for a Trimming Approach to Cluster Analysis

Directory of Open Access Journals (Sweden)

2012-04-01

Full Text Available Outlying data can heavily influence standard clustering methods. At the same time, clustering principles can be useful when robustifying statistical procedures. These two reasons motivate the development of feasible robust model-based clustering approaches. With this in mind, an R package for performing non-hierarchical robust clustering, called tclust, is presented here. Instead of trying to “fit” noisy data, a proportion α of the most outlying observations is trimmed. The tclust package efficiently handles different cluster scatter constraints. Graphical exploratory tools are also provided to help the user make sensible choices for the trimming proportion as well as the number of clusters to search for.

Trimming algorithm of frequency modulation for CIAE-230 MeV proton superconducting synchrocyclotron model cavity

Energy Technology Data Exchange (ETDEWEB)

Li, Pengzhan, E-mail: lipengzhan@ciae.ac.cn; Zhang, Tianjue; Ji, Bin; Hou, Shigang; Guo, Juanjuan; Yin, Meng; Xing, Jiansheng; Lv, Yinlong; Guan, Fengping; Lin, Jun

2017-01-21

A new project, the 230 MeV proton superconducting synchrocyclotron for cancer therapy, was proposed at CIAE in 2013. A model cavity is designed to verify the frequency modulation trimming algorithm featuring a half-wave structure and eight sets of rotating blades for 1 kHz frequency modulation. Based on the electromagnetic (EM) field distribution analysis of the model cavity, the variable capacitor works as a function of time and the frequency can be written in Maclaurin series. Curve fitting is applied for theoretical frequency and original simulation frequency. The second-order fitting excels at the approximation given its minimum variance. Constant equivalent inductance is considered as an important condition in the calculation. The equivalent parameters of theoretical frequency can be achieved through this conversion. Then the trimming formula for rotor blade outer radius is found by discretization in time domain. Simulation verification has been performed and the results show that the calculation radius with minus 0.012 m yields an acceptable result. The trimming amendment in the time range of 0.328–0.4 ms helps to reduce the frequency error to 0.69% in Simulation C with an increment of 0.075 mm/0.001 ms, which is half of the error in Simulation A (constant radius in 0.328–0.4 ms). The verification confirms the feasibility of the trimming algorithm for synchrocyclotron frequency modulation. - Highlights: • A model cavity is designed to verify the trimming algorithm of frequency modulation. • The RF frequency is expressed by fitting approximation and Maclaurin series. • The variable capacitor of the cavity works as a function of time. • The trimming formula for blade radius is found by discretization in time domain. • The amendment solution helps to reduce the frequency error.

Integral Design methodology for Industrial Collaboration Design of Sustainable Industrial Flexible Demountable buildings

NARCIS (Netherlands)

Zeiler, W.; Quanjel, E.M.C.J.; Bauer, M.; Lima, C.

2007-01-01

Starting in 1998 from developing and designing their own office Kropman, a major Dutch Building Services contractor, developed a new methodology for structuring and documenting integral design processes. Integral design is meant to integrate the different disciplines involved in the building design

Design of an Integrated Methodology for Analytical Design of Complex Supply Chains

Directory of Open Access Journals (Sweden)

Shahid Rashid

2012-01-01

Full Text Available A literature review and gap analysis indentifies key limitations of industry best practice when modelling of supply chains. To address these limitations the paper reports on the conception and development of an integrated modelling methodology designed to underpin the analytical design of complex supply chains. The methodology is based upon a systematic deployment of EM, CLD, and SM techniques; the integration of which is achieved via common modelling concepts and decomposition principles. Thereby the methodology facilitates: (i graphical representation and description of key “processing”, “resourcing” and “work flow” properties of supply chain configurations; (ii behavioural exploration of currently configured supply chains, to facilitate reasoning about uncertain demand impacts on supply, make, delivery, and return processes; (iii predictive quantification about relative performances of alternative complex supply chain configurations, including risk assessments. Guidelines for the application of each step of the methodology are described. Also described are recommended data collection methods and expected modelling outcomes for each step. The methodology is being extensively case tested to quantify potential benefits & costs relative to current best industry practice. The paper reflects on preliminary benefits gained during industry based case study modelling and identifies areas of potential improvement.

FTR83, a Member of the Large Fish-Specific finTRIM Family, Triggers IFN Pathway and Counters Viral Infection

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Christelle Langevin

2017-05-01

Full Text Available Tripartite motif (TRIM proteins are involved in various cellular functions and constitute key factors of the antiviral innate immune response. TRIM proteins can bind viral particles directly, sending them to degradation by the proteasome, or ubiquitinate signaling molecules leading to upregulation of innate immunity. TRIM proteins are present in across metazoans but are particularly numerous in vertebrates where genes comprising a B30.2 domain have been often duplicated. In fish, a TRIM subset named finTRIM is highly diversified, with large gene numbers and clear signatures of positive selection in the B30.2 domain suggesting they may be involved in antiviral mechanisms. finTRIM provides a beautiful model to investigate the primordial implication of B30.2 TRIM subsets in the arsenal of vertebrate antiviral defenses. We show here that ftr83, a zebrafish fintrim gene mainly expressed in the gills, skin and pharynx, encodes a protein affording a potent antiviral activity. In vitro, overexpression of FTR83, but not of its close relative FTR82, induced IFN and IFN-stimulated gene expression and afforded protection against different enveloped and non-enveloped RNA viruses. The kinetics of IFN induction paralleled the development of the antiviral activity, which was abolished by a dominant negative IRF3 mutant. In the context of a viral infection, FTR83 potentiated the IFN response. Expression of chimeric proteins in which the B30.2 domain of FTR83 and the non-protective FTR82 had been exchanged, showed that IFN upregulation and antiviral activity requires both the Ring/BBox/Coiled coil domain (supporting E3 ubiquitin ligase and the B30.2 domain of FTR83. Finally, loss of function experiments in zebrafish embryos confirms that ftr83 mediates antiviral activity in vivo. Our results show that a member of the largest TRIM subset observed in fish upregulates type I IFN response and afford protection against viral infections, supporting that TRIMs are key

TRIM30α Is a Negative-Feedback Regulator of the Intracellular DNA and DNA Virus-Triggered Response by Targeting STING.

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Yanming Wang

2015-06-01

Full Text Available Uncontrolled immune responses to intracellular DNA have been shown to induce autoimmune diseases. Homeostasis regulation of immune responses to cytosolic DNA is critical for limiting the risk of autoimmunity and survival of the host. Here, we report that the E3 ubiquitin ligase tripartite motif protein 30α (TRIM30α was induced by herpes simplex virus type 1 (HSV-1 infection in dendritic cells (DCs. Knockdown or genetic ablation of TRIM30α augmented the type I IFNs and interleukin-6 response to intracellular DNA and DNA viruses. Trim30α-deficient mice were more resistant to infection by DNA viruses. Biochemical analyses showed that TRIM30α interacted with the stimulator of interferon genes (STING, which is a critical regulator of the DNA-sensing response. Overexpression of TRIM30α promoted the degradation of STING via K48-linked ubiquitination at Lys275 through a proteasome-dependent pathway. These findings indicate that E3 ligase TRIM30α is an important negative-feedback regulator of innate immune responses to DNA viruses by targeting STING.

The Salmonella Effector Protein SopA Modulates Innate Immune Responses by Targeting TRIM E3 Ligase Family Members.

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Jana Kamanova

2016-04-01

Full Text Available Salmonella Typhimurium stimulates inflammatory responses in the intestinal epithelium, which are essential for its ability to replicate within the intestinal tract. Stimulation of these responses is strictly dependent on the activity of a type III secretion system encoded within its pathogenicity island 1, which through the delivery of effector proteins, triggers signaling pathways leading to inflammation. One of these effectors is SopA, a HECT-type E3 ligase, which is required for the efficient stimulation of inflammation in an animal model of Salmonella Typhimurium infection. We show here that SopA contributes to the stimulation of innate immune responses by targeting two host E3 ubiquitin ligases, TRIM56 and TRIM65. We also found that TRIM65 interacts with the innate immune receptor MDA5 enhancing its ability to stimulate interferon-β signaling. Therefore, by targeting TRIM56 and TRIM65, SopA can stimulate signaling through two innate immune receptors, RIG-I and MDA5. These findings describe a Salmonella mechanism to modulate inflammatory responses by directly targeting innate immune signaling mechanisms.

Evaluation of the UnTRIM model for 3-D tidal circulation

Science.gov (United States)

Cheng, R.T.; Casulli, V.; ,

2001-01-01

A family of numerical models, known as the TRIM models, shares the same modeling philosophy for solving the shallow water equations. A characteristic analysis of the shallow water equations points out that the numerical instability is controlled by the gravity wave terms in the momentum equations and by the transport terms in the continuity equation. A semi-implicit finite-difference scheme has been formulated so that these terms and the vertical diffusion terms are treated implicitly and the remaining terms explicitly to control the numerical stability and the computations are carried out over a uniform finite-difference computational mesh without invoking horizontal or vertical coordinate transformations. An unstructured grid version of TRIM model is introduced, or UnTRIM (pronounces as "you trim"), which preserves these basic numerical properties and modeling philosophy, only the computations are carried out over an unstructured orthogonal grid. The unstructured grid offers the flexibilities in representing complex study areas so that fine grid resolution can be placed in regions of interest, and coarse grids are used to cover the remaining domain. Thus, the computational efforts are concentrated in areas of importance, and an overall computational saving can be achieved because the total number of grid-points is dramatically reduced. To use this modeling approach, an unstructured grid mesh must be generated to properly reflect the properties of the domain of the investigation. The new modeling flexibility in grid structure is accompanied by new challenges associated with issues of grid generation. To take full advantage of this new model flexibility, the model grid generation should be guided by insights into the physics of the problems; and the insights needed may require a higher degree of modeling skill.

Lead intoxication in dogs: risk assessment of feeding dogs trimmings of lead-shot game.

Science.gov (United States)

Høgåsen, Helga R; Ørnsrud, Robin; Knutsen, Helle K; Bernhoft, Aksel

2016-07-25

Expanding lead-based bullets, commonly used for hunting of big game, produce a scattering of lead particles in the carcass around the wound channel. Trimmings around this channel, which are sometimes fed to dogs, may contain lead particles. The aim of this study was to assess potential health effects of feeding dogs such trimmings. Lead ingestion most commonly causes gastrointestinal and neurological clinical signs, although renal, skeletal, haematological, cardiovascular and biochemical effects have also been reported. Experimental data indicate that a daily dose of around 1 mg lead as lead acetate/kg body weight for ten days may be considered as a Lowest Observed Effect Level in dogs. Acute toxicity documentation from the Centers for Disease Control and Prevention indicates 300 mg/kg body weight as the lowest dose of lead acetate causing death in dogs after oral ingestion. Our assessment suggests that dogs fed trimmings of lead-shot game may be affected by the amounts of lead present, and that even deadly exposure could occasionally occur. The intestinal absorption of lead from bullets was assumed to be 10-80 % of that of lead acetate, reflecting both the variability in particle size and uncertainty about the bioavailability of metallic lead in dogs. Despite data gaps, this study indicates that feeding dogs trimmings of lead-shot game may represent a risk of lead intoxication. More research is needed to assess the exact consequences, if lead-based bullets are still to be used. Meanwhile, we recommend that trimmings close to the wound channel should be made inaccessible to dogs, as well as to other domestic or wild animals.

Body-induced vortical flows: a common mechanism for self-corrective trimming control in boxfishes.

Science.gov (United States)

Bartol, Ian K; Gharib, Morteza; Webb, Paul W; Weihs, Daniel; Gordon, Malcolm S

2005-01-01

Boxfishes (Teleostei: Ostraciidae) are marine fishes having rigid carapaces that vary significantly among taxa in their shapes and structural ornamentation. We showed previously that the keels of the carapace of one species of tropical boxfish, the smooth trunkfish, produce leading edge vortices (LEVs) capable of generating self-correcting trimming forces during swimming. In this paper we show that other tropical boxfishes with different carapace shapes have similar capabilities. We conducted a quantitative study of flows around the carapaces of three morphologically distinct boxfishes (spotted boxfish, scrawled cowfish and buffalo trunkfish) using stereolithographic models and three separate but interrelated analytical approaches: digital particle image velocimetry (DPIV), pressure distribution measurements, and force balance measurements. The ventral keels of all three forms produced LEVs that grew in circulation along the bodies, resembling the LEVs produced around delta-winged aircraft. These spiral vortices formed above the keels and increased in circulation as pitch angle became more positive, and formed below the keels and increased in circulation as pitch angle became more negative. Vortices also formed along the eye ridges of all boxfishes. In the spotted boxfish, which is largely trapezoidal in cross section, consistent dorsal vortex growth posterior to the eye ridge was also present. When all three boxfishes were positioned at various yaw angles, regions of strongest concentrated vorticity formed in far-field locations of the carapace compared with near-field areas, and vortex circulation was greatest posterior to the center of mass. In general, regions of localized low pressure correlated well with regions of attached, concentrated vorticity, especially around the ventral keels. Although other features of the carapace also affect flow patterns and pressure distributions in different ways, the integrated effects of the flows were consistent for all forms

The Host E3-Ubiquitin Ligase TRIM6 Ubiquitinates the Ebola Virus VP35 Protein and Promotes Virus Replication.

Science.gov (United States)

Bharaj, Preeti; Atkins, Colm; Luthra, Priya; Giraldo, Maria Isabel; Dawes, Brian E; Miorin, Lisa; Johnson, Jeffrey R; Krogan, Nevan J; Basler, Christopher F; Freiberg, Alexander N; Rajsbaum, Ricardo

2017-09-15

Ebola virus (EBOV), a member of the Filoviridae family, is a highly pathogenic virus that causes severe hemorrhagic fever in humans and is responsible for epidemics throughout sub-Saharan, central, and West Africa. The EBOV genome encodes VP35, an important viral protein involved in virus replication by acting as an essential cofactor of the viral polymerase as well as a potent antagonist of the host antiviral type I interferon (IFN-I) system. By using mass spectrometry analysis and coimmunoprecipitation assays, we show here that VP35 is ubiquitinated on lysine 309 (K309), a residue located on its IFN antagonist domain. We also found that VP35 interacts with TRIM6, a member of the E3-ubiquitin ligase tripartite motif (TRIM) family. We recently reported that TRIM6 promotes the synthesis of unanchored K48-linked polyubiquitin chains, which are not covalently attached to any protein, to induce efficient antiviral IFN-I-mediated responses. Consistent with this notion, VP35 also associated noncovalently with polyubiquitin chains and inhibited TRIM6-mediated IFN-I induction. Intriguingly, we also found that TRIM6 enhances EBOV polymerase activity in a minigenome assay and TRIM6 knockout cells have reduced replication of infectious EBOV, suggesting that VP35 hijacks TRIM6 to promote EBOV replication through ubiquitination. Our work provides evidence that TRIM6 is an important host cellular factor that promotes EBOV replication, and future studies will focus on whether TRIM6 could be targeted for therapeutic intervention against EBOV infection. IMPORTANCE EBOV belongs to a family of highly pathogenic viruses that cause severe hemorrhagic fever in humans and other mammals with high mortality rates (40 to 90%). Because of its high pathogenicity and lack of licensed antivirals and vaccines, EBOV is listed as a tier 1 select-agent risk group 4 pathogen. An important mechanism for the severity of EBOV infection is its suppression of innate immune responses. The EBOV VP35

UPCaD: A Methodology of Integration Between Ontology-Based Context-Awareness Modeling and Relational Domain Data

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Vinícius Maran

2018-01-01

Full Text Available Context-awareness is a key feature for ubiquitous computing scenarios applications. Currently, technologies and methodologies have been proposed for the integration of context-awareness concepts in intelligent information systems to adapt them to the execution of services, user interfaces and data retrieval. Recent research proposed conceptual modeling alternatives to the integration of the domain modeling in RDBMS and context-awareness modeling. The research described using highly expressiveness ontologies. The present work describes the UPCaD (Unified Process for Integration between Context-Awareness and Domain methodology, which is composed of formalisms and processes to guide the data integration considering RDBMS and context modeling. The methodology was evaluated in a virtual learning environment application. The evaluation shows the possibility to use a highly expressive context ontology to filter the relational data query and discusses the main contributions of the methodology compared with recent approaches.

Recovering probabilities for nucleotide trimming processes for T cell receptor TRA and TRG V-J junctions analyzed with IMGT tools

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Lefranc Marie-Paule

2008-10-01

Full Text Available Abstract Background Nucleotides are trimmed from the ends of variable (V, diversity (D and joining (J genes during immunoglobulin (IG and T cell receptor (TR rearrangements in B cells and T cells of the immune system. This trimming is followed by addition of nucleotides at random, forming the N regions (N for nucleotides of the V-J and V-D-J junctions. These processes are crucial for creating diversity in the immune response since the number of trimmed nucleotides and the number of added nucleotides vary in each B or T cell. IMGT® sequence analysis tools, IMGT/V-QUEST and IMGT/JunctionAnalysis, are able to provide detailed and accurate analysis of the final observed junction nucleotide sequences (tool "output". However, as trimmed nucleotides can potentially be replaced by identical N region nucleotides during the process, the observed "output" represents a biased estimate of the "true trimming process." Results A probabilistic approach based on an analysis of the standardized tool "output" is proposed to infer the probability distribution of the "true trimmming process" and to provide plausible biological hypotheses explaining this process. We collated a benchmark dataset of TR alpha (TRA and TR gamma (TRG V-J rearranged sequences and junctions analysed with IMGT/V-QUEST and IMGT/JunctionAnalysis, the nucleotide sequence analysis tools from IMGT®, the international ImMunoGeneTics information system®, http://imgt.cines.fr. The standardized description of the tool output is based on the IMGT-ONTOLOGY axioms and concepts. We propose a simple first-order model that attempts to transform the observed "output" probability distribution into an estimate closer to the "true trimming process" probability distribution. We use this estimate to test the hypothesis that Poisson processes are involved in trimming. This hypothesis was not rejected at standard confidence levels for three of the four trimming processes: TRAV, TRAJ and TRGV. Conclusion By

Recovering probabilities for nucleotide trimming processes for T cell receptor TRA and TRG V-J junctions analyzed with IMGT tools.

Science.gov (United States)

Bleakley, Kevin; Lefranc, Marie-Paule; Biau, Gérard

2008-10-02

Nucleotides are trimmed from the ends of variable (V), diversity (D) and joining (J) genes during immunoglobulin (IG) and T cell receptor (TR) rearrangements in B cells and T cells of the immune system. This trimming is followed by addition of nucleotides at random, forming the N regions (N for nucleotides) of the V-J and V-D-J junctions. These processes are crucial for creating diversity in the immune response since the number of trimmed nucleotides and the number of added nucleotides vary in each B or T cell. IMGT sequence analysis tools, IMGT/V-QUEST and IMGT/JunctionAnalysis, are able to provide detailed and accurate analysis of the final observed junction nucleotide sequences (tool "output"). However, as trimmed nucleotides can potentially be replaced by identical N region nucleotides during the process, the observed "output" represents a biased estimate of the "true trimming process." A probabilistic approach based on an analysis of the standardized tool "output" is proposed to infer the probability distribution of the "true trimmming process" and to provide plausible biological hypotheses explaining this process. We collated a benchmark dataset of TR alpha (TRA) and TR gamma (TRG) V-J rearranged sequences and junctions analysed with IMGT/V-QUEST and IMGT/JunctionAnalysis, the nucleotide sequence analysis tools from IMGT, the international ImMunoGeneTics information system, http://imgt.cines.fr. The standardized description of the tool output is based on the IMGT-ONTOLOGY axioms and concepts. We propose a simple first-order model that attempts to transform the observed "output" probability distribution into an estimate closer to the "true trimming process" probability distribution. We use this estimate to test the hypothesis that Poisson processes are involved in trimming. This hypothesis was not rejected at standard confidence levels for three of the four trimming processes: TRAV, TRAJ and TRGV. By using trimming of rearranged TR genes as a benchmark, we

The effect of preventive trimming on weight bearing and force balance on the claws of dairy cattle

NARCIS (Netherlands)

Tol, van der P.P.J.; Beek, van der S.S.; Metz, J.H.M.; Noordhuizen-Stassen, E.N.; Back, E.; Braam, C.R.; Weijs, W.A.

2004-01-01

Claw disorders and lameness in dairy cattle are an increasing problem of the modern dairy industry. To prevent claw disorders from evolving from the subclinical to the clinical stage, trimming is the management practice most routinely applied. The goal of preventive trimming (Toussaint-Raven method)

Frequent silencing of the candidate tumor suppressor TRIM58 by promoter methylation in early-stage lung adenocarcinoma.

Science.gov (United States)

Kajiura, Koichiro; Masuda, Kiyoshi; Naruto, Takuya; Kohmoto, Tomohiro; Watabnabe, Miki; Tsuboi, Mitsuhiro; Takizawa, Hiromitsu; Kondo, Kazuya; Tangoku, Akira; Imoto, Issei

2017-01-10

In this study, we aimed to identify novel drivers that would be epigenetically altered through aberrant methylation in early-stage lung adenocarcinoma (LADC), regardless of the presence or absence of tobacco smoking-induced epigenetic field defects. Through genome-wide screening for aberrantly methylated CpG islands (CGIs) in 12 clinically uniform, stage-I LADC cases affecting six non-smokers and six smokers, we identified candidate tumor-suppressor genes (TSGs) inactivated by hypermethylation. Through systematic expression analyses of those candidates in panels of additional tumor samples and cell lines treated or not treated with 5-aza-deoxycitidine followed by validation analyses of cancer-specific silencing by CGI hypermethylation using a public database, we identified TRIM58 as the most prominent candidate for TSG. TRIM58 was robustly silenced by hypermethylation even in early-stage primary LADC, and the restoration of TRIM58 expression in LADC cell lines inhibited cell growth in vitro and in vivo in anchorage-dependent and -independent manners. Our findings suggest that aberrant inactivation of TRIM58 consequent to CGI hypermethylation might stimulate the early carcinogenesis of LADC regardless of smoking status; furthermore, TRIM58 methylation might be a possible early diagnostic and epigenetic therapeutic target in LADC.

TRIM5 suppresses cross-species transmission of a primate immunodeficiency virus and selects for emergence of resistant variants in the new species.

Directory of Open Access Journals (Sweden)

Andrea Kirmaier

2010-08-01

Full Text Available Simian immunodeficiency viruses of sooty mangabeys (SIVsm are the source of multiple, successful cross-species transmissions, having given rise to HIV-2 in humans, SIVmac in rhesus macaques, and SIVstm in stump-tailed macaques. Cellular assays and phylogenetic comparisons indirectly support a role for TRIM5alpha, the product of the TRIM5 gene, in suppressing interspecies transmission and emergence of retroviruses in nature. Here, we investigate the in vivo role of TRIM5 directly, focusing on transmission of primate immunodeficiency viruses between outbred primate hosts. Specifically, we retrospectively analyzed experimental cross-species transmission of SIVsm in two cohorts of rhesus macaques and found a significant effect of TRIM5 genotype on viral replication levels. The effect was especially pronounced in a cohort of animals infected with SIVsmE543-3, where TRIM5 genotype correlated with approximately 100-fold to 1,000-fold differences in viral replication levels. Surprisingly, transmission occurred even in individuals bearing restrictive TRIM5 genotypes, resulting in attenuation of replication rather than an outright block to infection. In cell-culture assays, the same TRIM5 alleles associated with viral suppression in vivo blocked infectivity of two SIVsm strains, but not the macaque-adapted strain SIVmac239. Adaptations appeared in the viral capsid in animals with restrictive TRIM5 genotypes, and similar adaptations coincide with emergence of SIVmac in captive macaques in the 1970s. Thus, host TRIM5 can suppress viral replication in vivo, exerting selective pressure during the initial stages of cross-species transmission.

Prognostic indicators and the importance of trimming in noninfective ...

African Journals Online (AJOL)

Prognostic indicators and the importance of trimming in noninfective claw disorders in cattle. ... features of chronic laminitis occurred concurrently with heel or sole erosion, white line separation, sole ulcers, horizontal hoof wall fissures and double soles. In most smallholder farms, cows with these disorders were neglected to ...

Development of a methodology for conducting an integrated HRA/PRA --

Energy Technology Data Exchange (ETDEWEB)

Luckas, W.J.; Barriere, M.T.; Brown, W.S. (Brookhaven National Lab., Upton, NY (United States)); Wreathall, J. (Wreathall (John) and Co., Dublin, OH (United States)); Cooper, S.E. (Science Applications International Corp., McLean, VA (United States))

1993-01-01

During Low Power and Shutdown (LP S) conditions in a nuclear power plant (i.e., when the reactor is subcritical or at less than 10--15% power), human interactions with the plant's systems will be more frequent and more direct. Control is typically not mediated by automation, and there are fewer protective systems available. Therefore, an assessment of LP S related risk should include a greater emphasis on human reliability than such an assessment made for power operation conditions. In order to properly account for the increase in human interaction and thus be able to perform a probabilistic risk assessment (PRA) applicable to operations during LP S, it is important that a comprehensive human reliability assessment (HRA) methodology be developed and integrated into the LP S PRA. The tasks comprising the comprehensive HRA methodology development are as follows: (1) identification of the human reliability related influences and associated human actions during LP S, (2) identification of potentially important LP S related human actions and appropriate HRA framework and quantification methods, and (3) incorporation and coordination of methodology development with other integrated PRA/HRA efforts. This paper describes the first task, i.e., the assessment of human reliability influences and any associated human actions during LP S conditions for a pressurized water reactor (PWR).

Old trims for new: the cheaper solution to valve problems

Energy Technology Data Exchange (ETDEWEB)

Katz, B [Control Components, Inc., Rancho Santa Margarita, CA (United States)

1994-01-01

Rather than replacing a faulty valve - or one that is simply not up to the job - it has been possible to retrofit a variety of valve types used in nuclear plants with new trims which are described briefly. (Author).

Old trims for new: the cheaper solution to valve problems

International Nuclear Information System (INIS)

Katz, B.

1994-01-01

Rather than replacing a faulty valve - or one that is simply not up to the job - it has been possible to retrofit a variety of valve types used in nuclear plants with new trims which are described briefly. (Author)

PENGARUH KARAKTERISTIK PEKERJAAN DAN IKLIM KOMUNIKASI TERHADAP KEPUASAN KERJA KARYAWAN OPERATOR BAGIAN TRIMMING I PT KRAMA YUDHA RATU MOTOR

Directory of Open Access Journals (Sweden)

Agung AWS Waspodo

2013-09-01

Full Text Available The purpose of the research are: 1 To know the description of job characteristics, communication climate, and job satisfaction employees at trimming 1 PT Krama Yudha Ratu Motor. 2 To know about the influence of job characteristics toward job satisfaction trimming 1 employee’s at PT Krama Yudha Ratu Motor. 3 To know about the influence of communication climate toward job satisfaction trimming 1 employee’s at PT Krama Yudha Ratu Motor. 4 To know about the influence of job characteristics and communication climate toward job satisfaction trimming 1 employee’s at PT Krama Yudha Ratu Motor. The analysis implemented in this research are descriptive and explanatory analysis. This research is taken 71 trimming 1 employee’s at PT Krama Yudha Ratu Motor. While data collection by using questionnaire and cultivated processed using SPSS 16. The result showed that: 1 Job characteristics has a significant influence on job satisfaction. 2 communication climate has a significant influence on job satisfaction. 3 job characteristics and communication climate has a significant influence on job satisfaction.

Some aspects of Trim-algorithm modernization for Monte-Carlo method

International Nuclear Information System (INIS)

Dovnar, S.V.; Grigor'ev, V.V.; Kamyshan, M.A.; Leont'ev, A.V.; Yanusko, S.V.

2001-01-01

Some aspects of Trim-algorithm modernization in Monte-Carlo method are discussed. This modification permits to raise the universality of program work with various potentials of ion-atom interactions and to improve the calculation precision for scattering angle θ c

Aircraft interior noise models - Sidewall trim, stiffened structures, and cabin acoustics with floor partition

Science.gov (United States)

Pope, L. D.; Wilby, E. G.; Willis, C. M.; Mayes, W. H.

1983-01-01

As part of the continuing development of an aircraft interior noise prediction model, in which a discrete modal representation and power flow analysis are used, theoretical results are considered for inclusion of sidewall trim, stiffened structures, and cabin acoustics with floor partition. For validation purposes, predictions of the noise reductions for three test articles (a bare ring-stringer stiffened cylinder, an unstiffened cylinder with floor and insulation, and a ring-stringer stiffened cylinder with floor and sidewall trim) are compared with measurements.

SseK3 Is a Salmonella Effector That Binds TRIM32 and Modulates the Host's NF-κB Signalling Activity.

Directory of Open Access Journals (Sweden)

Zhe Yang

Full Text Available Salmonella Typhimurium employs an array of type III secretion system effectors that facilitate intracellular survival and replication during infection. The Salmonella effector SseK3 was originally identified due to amino acid sequence similarity with NleB; an effector secreted by EPEC/EHEC that possesses N-acetylglucoasmine (GlcNAc transferase activity and modifies death domain containing proteins to block extrinsic apoptosis. In this study, immunoprecipitation of SseK3 defined a novel molecular interaction between SseK3 and the host protein, TRIM32, an E3 ubiquitin ligase. The conserved DxD motif within SseK3, which is essential for the GlcNAc transferase activity of NleB, was required for TRIM32 binding and for the capacity of SseK3 to suppress TNF-stimulated activation of NF-κB pathway. However, we did not detect GlcNAc modification of TRIM32 by SseK3, nor did the SseK3-TRIM32 interaction impact on TRIM32 ubiquitination that is associated with its activation. In addition, lack of sseK3 in Salmonella had no effect on production of the NF-κB dependent cytokine, IL-8, in HeLa cells even though TRIM32 knockdown suppressed TNF-induced NF-κB activity. Ectopically expressed SseK3 partially co-localises with TRIM32 at the trans-Golgi network, but SseK3 is not recruited to Salmonella induced vacuoles or Salmonella induced filaments during Salmonella infection. Our study has identified a novel effector-host protein interaction and suggests that SseK3 may influence NF-κB activity. However, the lack of GlcNAc modification of TRIM32 suggests that SseK3 has further, as yet unidentified, host targets.

The KNOMAD Methodology for Integration of Multi-Disciplinary Engineering Knowledge within Aerospace Production

NARCIS (Netherlands)

Curran, R.; Verhagen, W.J.C.; Van Tooren, M.J.L.

2010-01-01

The paper is associated with the integration of multi-disciplinary knowledge within a Knowledge Based Engineering (KBE)-enabled design framework. To support this integration effort, the KNOMAD methodology has been devised. KNOMAD stands for Knowledge Optimized Manufacture And Design and is a

Integrating Shamanic Methodology into the Spirituality of Addictions Recovery Work

Science.gov (United States)

Rich, Marcia L.

2012-01-01

Responding to an increased recognition of the importance of spirituality in the aetiology and treatment of addictions, this article provides an overview of the potential contributions of both transpersonal psychology and shamanic methodology for the addictions field. A case study is provided to illustrate the integration of conventional,…

Applied methodology for replacement pipe arcs in integral pipelines TE 'Oslomej'

Directory of Open Access Journals (Sweden)

Temelkoska Bratica K.

2016-01-01

Full Text Available The integral pipelines in thermal power plants present a linear spatial bearing construction with high operating parameters, complex static and dynamic load. The integral pipelines along its entire length are hanging on construction spring hangers from the boiler building, where the boiler is placed, next to the machine hall where the turbine is placed. Therefore, it is important to monitor the condition and to remove any possible defects from the applied methods. This paper describes the methodology of replacement of the pipe arch on one of the integral pipelines-the line for hot superheated steam. In addition, in this paper are given the method methods that led to this methodology for testing and evaluation of the condition of the pipe arch material that had been in exploitation and the new pipe arch that will be embedded. Furthermore the approach, the technology of replacement, anchoring of the steam line, technology of welding etc., as well as the preparation of the final design of constructed condition are also covered in this paper.

An integrated methodology for characterizing flow and transport processes in fractured rock

International Nuclear Information System (INIS)

Wu, Yu-Shu

2007-01-01

To investigate the coupled processes involved in fluid and heat flow and chemical transport in the highly heterogeneous, unsaturated-zone (UZ) fractured rock of Yucca Mountain, we present an integrated modeling methodology. This approach integrates a wide variety of moisture, pneumatic, thermal, and geochemical isotopic field data into a comprehensive three-dimensional numerical model for modeling analyses. The results of field applications of the methodology show that moisture data, such as water potential and liquid saturation, are not sufficient to determine in situ percolation flux, whereas temperature and geochemical isotopic data provide better constraints to net infiltration rates and flow patterns. In addition, pneumatic data are found to be extremely valuable in estimating large-scale fracture permeability. The integration of hydrologic, pneumatic, temperature, and geochemical data into modeling analyses is thereby demonstrated to provide a practical modeling approach for characterizing flow and transport processes in complex fractured formations

Targeting TRIM5α in HIV Cure Strategies for the CRISPR-Cas9 Era

Directory of Open Access Journals (Sweden)

Daryl Anne Victoria Weatherley

2017-11-01

Full Text Available In the past decade, studies of innate immune activity against HIV-1 and other retroviruses have revealed a powerful array of host factors that can attack the virus at various stages of its life cycle in human and primate cells, raising the prospect that these antiviral factors could be manipulated in immunotherapeutic strategies for HIV infection. This has not proved straightforward: while HIV accessory genes encode proteins that subvert or destroy many of these restriction factors, others, such as human TRIM5α show limited potency against HIV-1. However, HIV-1 is much more susceptible to simian versions of TRIM5α: could this information be translated into the development of an effective gene therapy for HIV infection? Reigniting research into the restriction factor TRIM5α in the era of superior gene editing technology such as CRISPR-Cas9 presents an exciting opportunity to revisit this prospect.

UrQt: an efficient software for the Unsupervised Quality trimming of NGS data.

Science.gov (United States)

Modolo, Laurent; Lerat, Emmanuelle

2015-04-29

Quality control is a necessary step of any Next Generation Sequencing analysis. Although customary, this step still requires manual interventions to empirically choose tuning parameters according to various quality statistics. Moreover, current quality control procedures that provide a "good quality" data set, are not optimal and discard many informative nucleotides. To address these drawbacks, we present a new quality control method, implemented in UrQt software, for Unsupervised Quality trimming of Next Generation Sequencing reads. Our trimming procedure relies on a well-defined probabilistic framework to detect the best segmentation between two segments of unreliable nucleotides, framing a segment of informative nucleotides. Our software only requires one user-friendly parameter to define the minimal quality threshold (phred score) to consider a nucleotide to be informative, which is independent of both the experiment and the quality of the data. This procedure is implemented in C++ in an efficient and parallelized software with a low memory footprint. We tested the performances of UrQt compared to the best-known trimming programs, on seven RNA and DNA sequencing experiments and demonstrated its optimality in the resulting tradeoff between the number of trimmed nucleotides and the quality objective. By finding the best segmentation to delimit a segment of good quality nucleotides, UrQt greatly increases the number of reads and of nucleotides that can be retained for a given quality objective. UrQt source files, binary executables for different operating systems and documentation are freely available (under the GPLv3) at the following address: https://lbbe.univ-lyon1.fr/-UrQt-.html .

Genome-wide association study for claw disorders and trimming status in dairy cattle.

Science.gov (United States)

van der Spek, D; van Arendonk, J A M; Bovenhuis, H

2015-02-01

Performing a genome-wide association study (GWAS) might add to a better understanding of the development of claw disorders and the need for trimming. Therefore, the aim of the current study was to perform a GWAS on claw disorders and trimming status and to validate the results for claw disorders based on an independent data set. Data consisted of 20,474 cows with phenotypes for claw disorders and 50,238 cows with phenotypes for trimming status. Recorded claw disorders used in the current study were double sole (DS), interdigital hyperplasia (IH), sole hemorrhage (SH), sole ulcer (SU), white line separation (WLS), a combination of infectious claw disorders consisting of (inter-)digital dermatitis and heel erosion, and a combination of laminitis-related claw disorders (DS, SH, SU, and WLS). Of the cows with phenotypes for claw disorders, 1,771 cows were genotyped and these cow data were used for the GWAS on claw disorders. A SNP was considered significant when the false discovery rate≤0.05 and suggestive when the false discovery rate≤0.20. An independent data set of 185 genotyped bulls having at least 5 daughters with phenotypes (6,824 daughters in total) for claw disorders was used to validate significant and suggestive SNP detected based on the cow data. To analyze the trait "trimming status" (i.e., the need for claw trimming), a data set with 327 genotyped bulls having at least 5 daughters with phenotypes (18,525 daughters in total) was used. Based on the cow data, in total 10 significant and 45 suggestive SNP were detected for claw disorders. The 10 significant SNP were associated with SU, and mainly located on BTA8. The suggestive SNP were associated with DS, IH, SU, and laminitis-related claw disorders. Three of the suggestive SNP were validated in the data set of 185 bulls, and were located on BTA13, BTA14, and BTA17. For infectious claw disorders, SH, and WLS, no significant or suggestive SNP associations were detected. For trimming status, 1 significant

Integral Methodological Pluralism in Science Education Research: Valuing Multiple Perspectives

Science.gov (United States)

Davis, Nancy T.; Callihan, Laurie P.

2013-01-01

This article examines the multiple methodologies used in educational research and proposes a model that includes all of them as contributing to understanding educational contexts and research from multiple perspectives. The model, based on integral theory (Wilber in a theory of everything. Shambhala, Boston, 2000) values all forms of research as…

Reduced expression of TRIM21/Ro52 predicts poor prognosis in diffuse large B-cell lymphoma patients with and without rheumatic disease

DEFF Research Database (Denmark)

Brauner, S; Zhou, W; Backlin, C

2015-01-01

OBJECTIVE: TRIM21 (also known as Ro52) is an autoantigen in rheumatic disease and is predominantly expressed in leucocytes. Overexpression is associated with decreased proliferation, and the TRIM21 gene maps to a tumour suppressor locus. We therefore investigated the expression of TRIM21...... in patients with diffuse large B-cell lymphoma (DLBCL) and its potential usefulness as a prognostic biomarker. MATERIALS AND METHODS: TRIM21 expression levels were assessed by immunohistochemistry in lymphoma biopsies from three cohorts of patients with DLBCL: 42 patients with rheumatic disease treated...... with a cyclophosphamide, vincristine, doxorubicin and prednisone (CHOP)-like regimen, 76 CHOP-treated and 196 rituximab-CHOP-treated nonrheumatic patients. Expression was correlated with clinical and biomedical parameters. TRIM21 expression was assessed in relation to lymphocyte proliferation by quantitative PCR...

Scoring of digital dermatitis during milking as an alternative to scoring in a hoof trimming chute

DEFF Research Database (Denmark)

Thomsen, Peter; Klaas, Ilka Christine; Bach, K.

2008-01-01

Digital dermatitis is a serious problem in dairy production in many countries. In many settings, it is important to evaluate the digital dermatitis status of individual cows or an entire dairy herd. Such an evaluation has traditionally been done in a hoof trimming chute. An evaluation in the milk......Digital dermatitis is a serious problem in dairy production in many countries. In many settings, it is important to evaluate the digital dermatitis status of individual cows or an entire dairy herd. Such an evaluation has traditionally been done in a hoof trimming chute. An evaluation...... in the milking parlor can take place without disturbing the cows to a large extent, it can be done using less labor compared with an evaluation in a hoof trimming chute, and is cheaper than using a chute. The objective was to evaluate the sensitivity and specificity of a rapid screening method for digital...... dermatitis in the milking parlor, without using any specialized tools and taking approximately 15 s/cow. All lactating cows in 3 commercial Danish dairy herds were included. Cows were first scored for the presence of digital dermatitis during milking and the next day all cows were scored during hoof trimming...

Integrated Management System – Scope, Possibilities And Methodology

Directory of Open Access Journals (Sweden)

Čekanová Katarína

2015-06-01

Full Text Available Organizations are becoming more aware of the importance of integrated management systems (IMS. Interest in this subject indicates that IMS are seen as “management systems of the future”. Based on this, the aim of this articles characterizes the possibility of building IMS through the identification of common elements and specific requirements in accordance with the ISO 9001, ISO 14001 and OHSAS 18001 professional references. Part of the article is the methodology of building IMS in the organization.

Development of a methodology for conducting an integrated HRA/PRA --

International Nuclear Information System (INIS)

Luckas, W.J.; Barriere, M.T.; Brown, W.S.; Wreathall, J.; Cooper, S.E.

1993-01-01

During Low Power and Shutdown (LP ampersand S) conditions in a nuclear power plant (i.e., when the reactor is subcritical or at less than 10--15% power), human interactions with the plant's systems will be more frequent and more direct. Control is typically not mediated by automation, and there are fewer protective systems available. Therefore, an assessment of LP ampersand S related risk should include a greater emphasis on human reliability than such an assessment made for power operation conditions. In order to properly account for the increase in human interaction and thus be able to perform a probabilistic risk assessment (PRA) applicable to operations during LP ampersand S, it is important that a comprehensive human reliability assessment (HRA) methodology be developed and integrated into the LP ampersand S PRA. The tasks comprising the comprehensive HRA methodology development are as follows: (1) identification of the human reliability related influences and associated human actions during LP ampersand S, (2) identification of potentially important LP ampersand S related human actions and appropriate HRA framework and quantification methods, and (3) incorporation and coordination of methodology development with other integrated PRA/HRA efforts. This paper describes the first task, i.e., the assessment of human reliability influences and any associated human actions during LP ampersand S conditions for a pressurized water reactor (PWR)

Static Aeroelastic and Longitudinal Trim Model of Flexible Wing Aircraft Using Finite-Element Vortex-Lattice Coupled Solution

Science.gov (United States)

Ting, Eric; Nguyen, Nhan; Trinh, Khanh

2014-01-01

This paper presents a static aeroelastic model and longitudinal trim model for the analysis of a flexible wing transport aircraft. The static aeroelastic model is built using a structural model based on finite-element modeling and coupled to an aerodynamic model that uses vortex-lattice solution. An automatic geometry generation tool is used to close the loop between the structural and aerodynamic models. The aeroelastic model is extended for the development of a three degree-of-freedom longitudinal trim model for an aircraft with flexible wings. The resulting flexible aircraft longitudinal trim model is used to simultaneously compute the static aeroelastic shape for the aircraft model and the longitudinal state inputs to maintain an aircraft trim state. The framework is applied to an aircraft model based on the NASA Generic Transport Model (GTM) with wing structures allowed to flexibly deformed referred to as the Elastically Shaped Aircraft Concept (ESAC). The ESAC wing mass and stiffness properties are based on a baseline "stiff" values representative of current generation transport aircraft.

TRIMe7-CypA, an alternative splicing isoform of TRIMCyp in rhesus macaque, negatively modulates TRIM5α activity

Energy Technology Data Exchange (ETDEWEB)

Na, Lei [Harbin Veterinary Research Institute of the Chinese Academy of Agricultural Sciences, Harbin 150001 (China); Tang, Yan-Dong [Harbin Veterinary Research Institute of the Chinese Academy of Agricultural Sciences, Harbin 150001 (China); Biotechnology Institute of Southern Medical University, Guangzhou 510515 (China); Liu, Jian-Dong; Yu, Chang-Qing; Sun, Liu-Ke; Lin, Yue-Zhi; Wang, Xue-Feng [Harbin Veterinary Research Institute of the Chinese Academy of Agricultural Sciences, Harbin 150001 (China); Wang, Xiaojun, E-mail: xjw@hvri.ac.cn [Harbin Veterinary Research Institute of the Chinese Academy of Agricultural Sciences, Harbin 150001 (China); Zhou, Jian-Hua, E-mail: jianhua_uc@126.com [Harbin Veterinary Research Institute of the Chinese Academy of Agricultural Sciences, Harbin 150001 (China); Harbin Pharmaceutical Group Biovaccine Company, Harbin 150069 (China)

2014-04-04

Highlights: • TRIMe7-CypA expresses in rhesus and pig-tailed, but not long-tailed macaques. • TRIMe7-CypA does not show the restriction to a HIV-GFP report virus in vitro. • It acts as a negative modulator to TRIM5α likely by competitive inhibition. - Abstract: The existence of innate, host-specific restriction factors is a major obstacle to the development of nonhuman primate models for AIDS studies, and TRIM5α is one of the most important of these restriction factors. In recent years, a TRIM5 chimeric gene that was retrotransposed by a cyclophilin A (CypA) cDNA was identified in certain macaque species. The TRIM5α-CypA fusion protein, TRIMCyp, which was expressed in these monkeys, had lost its restriction ability toward HIV-1. We previously found that TRIMe7-CypA, an alternative splicing isoform of the TRIMCyp transcripts, was expressed in pig-tailed and rhesus macaques but absent in long-tailed macaques. In this study, the anti-HIV-1 activity of TRIMe7-CypA in the rhesus macaque (RhTRIMe7-CypA) was investigated. The over-expression of RhTRIMe7-CypA in CrFK, HeLa and HEK293T cells did not restrict the infection or replication of an HIV-1-GFP reporter virus in these cells. As a positive control, rhesus (rh)TRIM5α strongly inhibited the reporter virus. Intriguingly, the anti-HIV-1 activity of RhTRIM5α was significantly reduced in a dose-dependent manner by the co-repression of RhTRIMe7-CypA. Our data indicate that although the RhTRIMe7-CypA isoform does not appear to restrict HIV-1, it may act as a negative modulator of TRIM family proteins, presumably by competitive inhibition.

TRIMe7-CypA, an alternative splicing isoform of TRIMCyp in rhesus macaque, negatively modulates TRIM5α activity

International Nuclear Information System (INIS)

Na, Lei; Tang, Yan-Dong; Liu, Jian-Dong; Yu, Chang-Qing; Sun, Liu-Ke; Lin, Yue-Zhi; Wang, Xue-Feng; Wang, Xiaojun; Zhou, Jian-Hua

2014-01-01

Highlights: • TRIMe7-CypA expresses in rhesus and pig-tailed, but not long-tailed macaques. • TRIMe7-CypA does not show the restriction to a HIV-GFP report virus in vitro. • It acts as a negative modulator to TRIM5α likely by competitive inhibition. - Abstract: The existence of innate, host-specific restriction factors is a major obstacle to the development of nonhuman primate models for AIDS studies, and TRIM5α is one of the most important of these restriction factors. In recent years, a TRIM5 chimeric gene that was retrotransposed by a cyclophilin A (CypA) cDNA was identified in certain macaque species. The TRIM5α-CypA fusion protein, TRIMCyp, which was expressed in these monkeys, had lost its restriction ability toward HIV-1. We previously found that TRIMe7-CypA, an alternative splicing isoform of the TRIMCyp transcripts, was expressed in pig-tailed and rhesus macaques but absent in long-tailed macaques. In this study, the anti-HIV-1 activity of TRIMe7-CypA in the rhesus macaque (RhTRIMe7-CypA) was investigated. The over-expression of RhTRIMe7-CypA in CrFK, HeLa and HEK293T cells did not restrict the infection or replication of an HIV-1-GFP reporter virus in these cells. As a positive control, rhesus (rh)TRIM5α strongly inhibited the reporter virus. Intriguingly, the anti-HIV-1 activity of RhTRIM5α was significantly reduced in a dose-dependent manner by the co-repression of RhTRIMe7-CypA. Our data indicate that although the RhTRIMe7-CypA isoform does not appear to restrict HIV-1, it may act as a negative modulator of TRIM family proteins, presumably by competitive inhibition

Analyzing the Feasibility of Using Secure Application Integration Methodology (SAIM) for Integrating DON Enterprise Resource Planning (ERP) Applications

National Research Council Canada - National Science Library

Marin, Ramon

2004-01-01

...) would provide useful information about a beneficial methodology. SAIM is analyzed, by accessing its step by step directions, for suitability in the integration of the Enterprise Resource Planning (ERP...

Stable isogeometric analysis of trimmed geometries

Science.gov (United States)

Marussig, Benjamin; Zechner, Jürgen; Beer, Gernot; Fries, Thomas-Peter

2017-04-01

We explore extended B-splines as a stable basis for isogeometric analysis with trimmed parameter spaces. The stabilization is accomplished by an appropriate substitution of B-splines that may lead to ill-conditioned system matrices. The construction for non-uniform knot vectors is presented. The properties of extended B-splines are examined in the context of interpolation, potential, and linear elasticity problems and excellent results are attained. The analysis is performed by an isogeometric boundary element formulation using collocation. It is argued that extended B-splines provide a flexible and simple stabilization scheme which ideally suits the isogeometric paradigm.

Internal trim coils for CBA superconducting magnets

International Nuclear Information System (INIS)

Thompson, P.A.; Aronson, S.; Cottingham, J.G.; Garber, M.; Hahn, H.; Sampson, W.B.

1983-01-01

In order to correct iron saturation effects and shape the beam working line, superconducting trim coils have been constructed, which operate inside the main coils. Detailed studies of mechanical properties, quench behavior, fields produced, and hysteresis have lead to the production of accelerator-quality coils generating the required-strength harmonics up to cos (7theta). These are routinely installed in CBA main magnets and operate at 80% of short sample with negligible training in an ambient field of more than 5.3T

Comparison of some properties of four provisional restorations resin: Trim, Tempron, Acropars TRII & Duralay (Aria Dent

Directory of Open Access Journals (Sweden)

Monzavi A.

2006-08-01

Full Text Available Background and Aim: Use of provisional restorations is one of the important phases in the treatment of patients who need fixed prosthesis. Some physical properties are required for these materials. The purpose of this study was the comparison of some physical properties of 4 kinds of resins: Trim, Tempron, Duralay (Aria Dent and Acropars TRII. Materials and Methods: In this experimental study, transverse-strength (TS, setting time (ST, polymerization shrinkage (PS and temperature rise (TR during polymerization were tested. In transverse strength test, 5 samples of each resin were made. TS testing was done with an Instron testing machine. ST testing (5 samples in each group was done with Gilmore needle. In PS test, 11 cylindric samples were prepared from each resin and the shrinkage was examined with digital micrometer (up to 24 hours. In TR test, after mixing the powder and liquid of resins (8 samples in each group, the temperature rise was recorded with 10 seconds interval (up to 15 minutes. Data were analyzed with one-way ANOVA and Tukey HSD tests with p<0.05 as the limit of significance. Results: In TS test, all samples of Trim were bent and no fracture was observed, while Tempron and Duralay showed no significant difference. Both Tempron and Duralay revealed high significant difference with Acropars TRII (P <0.01. In ST test, the difference between groups was significant (P<0.01. The lowest ST was observed in Acropars TRII (7 min, and the highest in Tempron (9.53 min. In PS test, significant difference was observed between Trim and Acropars TRII (P<0.01. PS was completed after 120 min in Tempron and Trim groups. However, PS continued to increase in other groups. In TR test, the difference between groups was significant (P<0.05. Duralay showed the highest TR and the lowest was seen in Trim. Conclusion: In this study, Trim showed better properties than other studied groups. Acropars TRII had the lowest strength. The highest temperature rise

Application of integrated fuzzy VIKOR & AHP methodology to contractor ranking

Directory of Open Access Journals (Sweden)

Mohamad Rahim Ramezaniyan

2012-08-01

Full Text Available Contractor selection is a critical activity, which plays an important role in the overall success of any construction project. The implementation of fuzzy multiple criteria decision attribute (MCDA in selecting contractors has the advantage of rendering subjective and implicit decision making more objective and transparent. An additional merit of fuzzy MCDA is the ability to accommodate quantitative and qualitative information. In this paper, an integrated VIKOR–AHP methodology is proposed to make a selection among the alternative contractors in one of Iranian construction industry projects. In the proposed methodology, the weights of the selection criteria are determined by fuzzy pairwise comparison matrices of AHP.

The effect of routine hoof trimming on locomotion score, ruminating time, activity and milk yield of dairy cows

NARCIS (Netherlands)

Hertem, van T.; Parmet, Y.; Steensels, M.; Maltz, E.; Antler, A.; Schlageter Tello, A.A.; Lokhorst, C.; Romanini, C.E.B.; Viazzi, S.; Bahr, C.; Berckmans, D.; Halachmi, I.

2014-01-01

The objective of this study was to quantify the effect of hoof trimming on cow behavior (ruminating time, activity, and locomotion score) and performance (milk yield) over time. Data were gathered from a commercial dairy farm in Israel where routine hoof trimming is done by a trained hoof trimmer

A case on vendor selection methodology: An integrated approach

Directory of Open Access Journals (Sweden)

Nikhil C. Shil

2009-11-01

Full Text Available Vendor selection methodology is a highly researched area in supply chain management l terature and a very significant decision taken by supply chain managers due to technological advances in the manufacturing process. Such research has two basic dimensions: one is related to the identification of variables affecting the performance of the vendors and the other deals with the methodology to be applied. Most of the research conducted in this area deal with the upfront selection of vendors. However, it is very common to have a list of dedicated vendors due to the development of sophisticated production technologies like just in time (JIT, a lean or agile manufacturing process where continuous flow of materials is a requirement. This paper addresses the issue of selecting the optimal vendor from the internal database of a company. Factor analysis, analytical hierarchy process and regression analysis is used in an integrated way to supplement the vendor selection process. The methodology presented here is simply a proposal where every possible room for adjustment is available.

Resampling nucleotide sequences with closest-neighbor trimming and its comparison to other methods.

Directory of Open Access Journals (Sweden)

Kouki Yonezawa

Full Text Available A large number of nucleotide sequences of various pathogens are available in public databases. The growth of the datasets has resulted in an enormous increase in computational costs. Moreover, due to differences in surveillance activities, the number of sequences found in databases varies from one country to another and from year to year. Therefore, it is important to study resampling methods to reduce the sampling bias. A novel algorithm-called the closest-neighbor trimming method-that resamples a given number of sequences from a large nucleotide sequence dataset was proposed. The performance of the proposed algorithm was compared with other algorithms by using the nucleotide sequences of human H3N2 influenza viruses. We compared the closest-neighbor trimming method with the naive hierarchical clustering algorithm and [Formula: see text]-medoids clustering algorithm. Genetic information accumulated in public databases contains sampling bias. The closest-neighbor trimming method can thin out densely sampled sequences from a given dataset. Since nucleotide sequences are among the most widely used materials for life sciences, we anticipate that our algorithm to various datasets will result in reducing sampling bias.

Integrating rock mechanics issues with repository design through design process principles and methodology

International Nuclear Information System (INIS)

Bieniawski, Z.T.

1996-01-01

A good designer needs not only knowledge for designing (technical know-how that is used to generate alternative design solutions) but also must have knowledge about designing (appropriate principles and systematic methodology to follow). Concepts such as open-quotes design for manufactureclose quotes or open-quotes concurrent engineeringclose quotes are widely used in the industry. In the field of rock engineering, only limited attention has been paid to the design process because design of structures in rock masses presents unique challenges to the designers as a result of the uncertainties inherent in characterization of geologic media. However, a stage has now been reached where we are be able to sufficiently characterize rock masses for engineering purposes and identify the rock mechanics issues involved but are still lacking engineering design principles and methodology to maximize our design performance. This paper discusses the principles and methodology of the engineering design process directed to integrating site characterization activities with design, construction and performance of an underground repository. Using the latest information from the Yucca Mountain Project on geology, rock mechanics and starter tunnel design, the current lack of integration is pointed out and it is shown how rock mechanics issues can be effectively interwoven with repository design through a systematic design process methodology leading to improved repository performance. In essence, the design process is seen as the use of design principles within an integrating design methodology, leading to innovative problem solving. In particular, a new concept of open-quotes Design for Constructibility and Performanceclose quotes is introduced. This is discussed with respect to ten rock mechanics issues identified for repository design and performance

A trim-loss minimization in a produce-handling vehicle production plant

Directory of Open Access Journals (Sweden)

Apichai Ritvirool

2007-01-01

Full Text Available How to cut out the required pieces from raw materials by minimizing waste is a trim-loss problem. The integer linear programming (ILP model was developed to solve this problem. In addition, this ILPmodel could be used for planning an order over some future time period. Time horizon of ordering raw material including weekly, monthly, quarterly, and annually could be planned to reduce the trim loss. Thenumerical examples using an industrial case study of a produce-handling vehicle production plant were presented to illustrate how the proposed ILP model could be applied to actual systems and the types ofinformation that was obtained relative to implementation. The results showed that the proposed ILP model can be used as a decision support tool for selecting time horizon of order planning and cutting patterns todecrease material cost and waste from cutting raw material.

Helicopter Non-Unique Trim Strategies for Blade-Vortex Interaction (BVI) Noise Reduction

Science.gov (United States)

Malpica, Carlos; Greenwood, Eric; Sim, Ben W.

2016-01-01

An acoustics parametric analysis of the effect of fuselage drag and pitching moment on the Blade-Vortex Interaction (BVI) noise radiated by a medium lift helicopter (S-70UH-60) in a descending flight condition was conducted. The comprehensive analysis CAMRAD II was used for the calculation of vehicle trim, wake geometry and integrated air loads on the blade. The acoustics prediction code PSU-WOPWOP was used for calculating acoustic pressure signatures for a hemispherical grid centered at the hub. This paper revisits the concept of the X-force controller for BVI noise reduction, and investigates its effectiveness on an S-70 helicopter. The analysis showed that further BVI noise reductions were achievable by controlling the fuselage pitching moment. Reductions in excess of 6 dB of the peak BVI noise radiated towards the ground were demonstrated by compounding the effect of airframe drag and pitching moment simultaneously.

Propulsion integration of hypersonic air-breathing vehicles utilizing a top-down design methodology

Science.gov (United States)

Kirkpatrick, Brad Kenneth

In recent years, a focus of aerospace engineering design has been the development of advanced design methodologies and frameworks to account for increasingly complex and integrated vehicles. Techniques such as parametric modeling, global vehicle analyses, and interdisciplinary data sharing have been employed in an attempt to improve the design process. The purpose of this study is to introduce a new approach to integrated vehicle design known as the top-down design methodology. In the top-down design methodology, the main idea is to relate design changes on the vehicle system and sub-system level to a set of over-arching performance and customer requirements. Rather than focusing on the performance of an individual system, the system is analyzed in terms of the net effect it has on the overall vehicle and other vehicle systems. This detailed level of analysis can only be accomplished through the use of high fidelity computational tools such as Computational Fluid Dynamics (CFD) or Finite Element Analysis (FEA). The utility of the top-down design methodology is investigated through its application to the conceptual and preliminary design of a long-range hypersonic air-breathing vehicle for a hypothetical next generation hypersonic vehicle (NHRV) program. System-level design is demonstrated through the development of the nozzle section of the propulsion system. From this demonstration of the methodology, conclusions are made about the benefits, drawbacks, and cost of using the methodology.

International Comprehensive Ocean-Atmosphere Data Set (ICOADS) with Enhanced Trimming, Release 3

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — This dataset contains the latest official release of International Comprehensive Ocean-Atmosphere Data Set (ICOADS) with Enhanced Trimming, provided in a common...

Trimming and procrastination as inversion techniques

Science.gov (United States)

Backus, George E.

1996-12-01

By examining the processes of truncating and approximating the model space (trimming it), and by committing to neither the objectivist nor the subjectivist interpretation of probability (procrastinating), we construct a formal scheme for solving linear and non-linear geophysical inverse problems. The necessary prior information about the correct model xE can be either a collection of inequalities or a probability measure describing where xE was likely to be in the model space X before the data vector y0 was measured. The results of the inversion are (1) a vector z0 that estimates some numerical properties zE of xE; (2) an estimate of the error δz = z0 - zE. As y0 is finite dimensional, so is z0, and hence in principle inversion cannot describe all of xE. The error δz is studied under successively more specialized assumptions about the inverse problem, culminating in a complete analysis of the linear inverse problem with a prior quadratic bound on xE. Our formalism appears to encompass and provide error estimates for many of the inversion schemes current in geomagnetism, and would be equally applicable in geodesy and seismology if adequate prior information were available there. As an idealized example we study the magnetic field at the core-mantle boundary, using satellite measurements of field elements at sites assumed to be almost uniformly distributed on a single spherical surface. Magnetospheric currents are neglected and the crustal field is idealized as a random process with rotationally invariant statistics. We find that an appropriate data compression diagonalizes the variance matrix of the crustal signal and permits an analytic trimming of the idealized problem.

Fusion integral experiments and analysis and the determination of design safety factors - I: Methodology

International Nuclear Information System (INIS)

Youssef, M.Z.; Kumar, A.; Abdou, M.A.; Oyama, Y.; Maekawa, H.

1995-01-01

The role of the neutronics experimentation and analysis in fusion neutronics research and development programs is discussed. A new methodology was developed to arrive at estimates to design safety factors based on the experimental and analytical results from design-oriented integral experiments. In this methodology, and for a particular nuclear response, R, a normalized density function (NDF) is constructed from the prediction uncertainties, and their associated standard deviations, as found in the various integral experiments where that response, R, is measured. Important statistical parameters are derived from the NDF, such as the global mean prediction uncertainty, and the possible spread around it. The method of deriving safety factors from many possible NDFs based on various calculational and measuring methods (among other variants) is also described. Associated with each safety factor is a confidence level, designers may choose to have, that the calculated response, R, will not exceed (or will not fall below) the actual measured value. An illustrative example is given on how to construct the NDFs. The methodology is applied in two areas, namely the line-integrated tritium production rate and bulk shielding integral experiments. Conditions under which these factors could be derived and the validity of the method are discussed. 72 refs., 17 figs., 4 tabs

Precision-Trimming 2D Inverse-Opal Lattice on Elastomer to Ordered Nanostructures with Variable Size and Morphology.

Science.gov (United States)

Zhan, Haoran; Chen, Yanqiu; Liu, Yu; Lau, Woonming; Bao, Chao; Li, Minggan; Lu, Yunlong; Mei, Jun; Hui, David

2017-05-23

A low-cost and scalable method is developed for producing large-area elastomer surfaces having ordered nanostructures with a variety of lattice features controllable to nanometer precision. The method adopts the known technique of molding a PDMS precursor film with a close-packed monolayer of monodisperse submicron polystyrene beads on water to form an inverse-opal dimple lattice with the dimple size controlled by the bead selection and the dimple depth by the molding condition. The subsequent novel precision engineering of the inverse-opal lattice comprises trimming the PDMS precursor by a combination of polymer curing temperature/time and polymer dissolution parameters. The resultant ordered surface nanostructures, fabricated with an increasing degree of trimming, include (a) submicron hemispherical dimples with nanothin interdimple rims and walls; (b) nanocones with variable degrees of tip-sharpness by trimming off the top part of the nanothin interdimple walls; and (c) soup-plate-like submicron shallow dimples with interdimple rims and walls by anisotropically trimming off the nanocones and forming close-packed shallow dimples. As exemplars of industrial relevance of these lattice features, tunable Young's modulus and wettability are demonstrated.

Environmental integrated impact assessment for waste treatment activity: methodology and case-study application

International Nuclear Information System (INIS)

Lonati, G.; Panzeri, A.

2008-01-01

A literature method for the environmental integrated impact assessment, according to the IPPC Directive, has been critically analysed and adjusted in order to be used for the environmental performance assessment of waste treatment activities. The assessment parameters, sorted in eight treatment and combined pollution categories, have been partly redefined and re balanced. The adjusted methodology has been applied to a real case-study, a chemical- physical waste treatment plant, in order to calculate the current performance (Actual Integrated Index) and the ideal performance (Actual Integrated Index) achievable by technical and operational improvements. The adjusted methodology has also been used as a decision support system, in order to estimate the value of the expected environmental performances improvement after the execution achievable from the introduction of a single one or a set of improvement actions. The valuation of the Integrated Index percentage reduction, along with the action achievable, made the best actions able to be identified, both in comparative way and in the cost-effective one. The results, 50 as Effective Integrated Index and 42 as Ideal Integrated Index, in a 10-100 scale, show a medium impact level and point out an appreciable improvement margin on all the environmental performances, especially in air emission control and water consumption [it

The Integration of Project-Based Methodology into Teaching in Machine Translation

Science.gov (United States)

Madkour, Magda

2016-01-01

This quantitative-qualitative analytical research aimed at investigating the effect of integrating project-based teaching methodology into teaching machine translation on students' performance. Data was collected from the graduate students in the College of Languages and Translation, at Imam Muhammad Ibn Saud Islamic University, Riyadh, Saudi…

Statistical Methodologies to Integrate Experimental and Computational Research

Science.gov (United States)

Parker, P. A.; Johnson, R. T.; Montgomery, D. C.

2008-01-01

Development of advanced algorithms for simulating engine flow paths requires the integration of fundamental experiments with the validation of enhanced mathematical models. In this paper, we provide an overview of statistical methods to strategically and efficiently conduct experiments and computational model refinement. Moreover, the integration of experimental and computational research efforts is emphasized. With a statistical engineering perspective, scientific and engineering expertise is combined with statistical sciences to gain deeper insights into experimental phenomenon and code development performance; supporting the overall research objectives. The particular statistical methods discussed are design of experiments, response surface methodology, and uncertainty analysis and planning. Their application is illustrated with a coaxial free jet experiment and a turbulence model refinement investigation. Our goal is to provide an overview, focusing on concepts rather than practice, to demonstrate the benefits of using statistical methods in research and development, thereby encouraging their broader and more systematic application.

A robust regression based on weighted LSSVM and penalized trimmed squares

International Nuclear Information System (INIS)

Liu, Jianyong; Wang, Yong; Fu, Chengqun; Guo, Jie; Yu, Qin

2016-01-01

Least squares support vector machine (LS-SVM) for nonlinear regression is sensitive to outliers in the field of machine learning. Weighted LS-SVM (WLS-SVM) overcomes this drawback by adding weight to each training sample. However, as the number of outliers increases, the accuracy of WLS-SVM may decrease. In order to improve the robustness of WLS-SVM, a new robust regression method based on WLS-SVM and penalized trimmed squares (WLSSVM–PTS) has been proposed. The algorithm comprises three main stages. The initial parameters are obtained by least trimmed squares at first. Then, the significant outliers are identified and eliminated by the Fast-PTS algorithm. The remaining samples with little outliers are estimated by WLS-SVM at last. The statistical tests of experimental results carried out on numerical datasets and real-world datasets show that the proposed WLSSVM–PTS is significantly robust than LS-SVM, WLS-SVM and LSSVM–LTS.

TRIM timber projections: an evaluation based on forest inventory measurements.

Science.gov (United States)

John R. Mills

1989-01-01

Two consecutive timberland inventories collected from permanent plots in the natural pine type in North Carolina were used to evaluate the timber resource inventory model (TRIM). This study compares model predictions with field measurements and examines the effect of inventory data aggregation on the accuracy of projections. Projections were repeated for two geographic...

Design methodology for integrated downstream separation systems in an ethanol biorefinery

Science.gov (United States)

Mohammadzadeh Rohani, Navid

and obtaining energy security. On the other hand, Process Integration (PI) as defined by Natural Resource Canada as the combination of activities which aim at improving process systems, their unit operations and their interactions in order to maximize the efficiency of using water, energy and raw materials can also help biorefineries lower their energy consumptions and improve their economics. Energy integration techniques such as pinch analysis adopted by different industries over the years have ensured using heat sources within a plant to supply the demand internally and decrease the external utility consumption. Therefore, adopting energy integration can be one of the ways biorefinery technology owners can consider in their process development as well as their business model in order to improve their overall economics. The objective of this thesis is to propose a methodology for designing integrated downstream separation in a biorefinery. This methodology is tested in an ethanol biorefinery case study. Several alternative separation techniques are evaluated in their energy consumption and economics in three different scenarios; stand-alone without energy integration, stand-alone with internal energy integration and integrated-with Kraft. The energy consumptions and capital costs of separation techniques are assessed in each scenario and the cost and benefit of integration are determined and finally the best alternative is found through techno-economic metrics. Another advantage of this methodology is the use of a graphical tool which provides insights on decreasing energy consumption by modifying the process condition. The pivot point of this work is the use of a novel energy integration method called Bridge analysis. This systematic method which originally is intended for retrofit situation is used here for integration with Kraft process. Integration potentials are identified through this method and savings are presented for each design. In stand-alone with

Methodology of the Integrated Analysis of Company's Financial Status and Its Performance Results

OpenAIRE

Mackevičius, Jonas; Valkauskas, Romualdas

2010-01-01

Information about company's financial status and its performance results is very important for the objective evaluation of company's position in the market and competitive possibilities in the future. Such information is provided in the financial statement. It is important to apply and investigate this information properly. The methodology of company's financial status and performance results integrated analysis is recommended in this article. This methodology consists of these three elements...

Automotive Body Trim and Glass; Automotive Mechanics 2: 9045.05.

Science.gov (United States)

Dade County Public Schools, Miami, FL.

This document presents an outline for a 135-hour course designed to help students become employable with skills, knowledge, attitudes, and values necessary for performing the required service of the automotive trim and glass mechanic. The course of study includes an orientation to the course, service tools and bench skills development, and a study…

An integrated methodology for the dynamic performance and reliability evaluation of fault-tolerant systems

International Nuclear Information System (INIS)

Dominguez-Garcia, Alejandro D.; Kassakian, John G.; Schindall, Joel E.; Zinchuk, Jeffrey J.

2008-01-01

We propose an integrated methodology for the reliability and dynamic performance analysis of fault-tolerant systems. This methodology uses a behavioral model of the system dynamics, similar to the ones used by control engineers to design the control system, but also incorporates artifacts to model the failure behavior of each component. These artifacts include component failure modes (and associated failure rates) and how those failure modes affect the dynamic behavior of the component. The methodology bases the system evaluation on the analysis of the dynamics of the different configurations the system can reach after component failures occur. For each of the possible system configurations, a performance evaluation of its dynamic behavior is carried out to check whether its properties, e.g., accuracy, overshoot, or settling time, which are called performance metrics, meet system requirements. Markov chains are used to model the stochastic process associated with the different configurations that a system can adopt when failures occur. This methodology not only enables an integrated framework for evaluating dynamic performance and reliability of fault-tolerant systems, but also enables a method for guiding the system design process, and further optimization. To illustrate the methodology, we present a case-study of a lateral-directional flight control system for a fighter aircraft

How to conduct a qualitative meta-analysis: Tailoring methods to enhance methodological integrity.

Science.gov (United States)

Levitt, Heidi M

2018-05-01

Although qualitative research has long been of interest in the field of psychology, meta-analyses of qualitative literatures (sometimes called meta-syntheses) are still quite rare. Like quantitative meta-analyses, these methods function to aggregate findings and identify patterns across primary studies, but their aims, procedures, and methodological considerations may vary. This paper explains the function of qualitative meta-analyses and their methodological development. Recommendations have broad relevance but are framed with an eye toward their use in psychotherapy research. Rather than arguing for the adoption of any single meta-method, this paper advocates for considering how procedures can best be selected and adapted to enhance a meta-study's methodological integrity. Through the paper, recommendations are provided to help researchers identify procedures that can best serve their studies' specific goals. Meta-analysts are encouraged to consider the methodological integrity of their studies in relation to central research processes, including identifying a set of primary research studies, transforming primary findings into initial units of data for a meta-analysis, developing categories or themes, and communicating findings. The paper provides guidance for researchers who desire to tailor meta-analytic methods to meet their particular goals while enhancing the rigor of their research.

Laser Trimming of CuAlMo Thin-Film Resistors: Effect of Laser Processing Parameters

Science.gov (United States)

Birkett, Martin; Penlington, Roger

2012-08-01

This paper reports the effect of varying laser trimming process parameters on the electrical performance of a novel CuAlMo thin-film resistor material. The films were prepared on Al2O3 substrates by direct-current (DC) magnetron sputtering, before being laser trimmed to target resistance value. The effect of varying key laser parameters of power, Q-rate, and bite size on the resistor stability and tolerance accuracy were systematically investigated. By reducing laser power and bite size and balancing this with Q-rate setting, significant improvements in resistor stability and resistor tolerance accuracies of less than ±0.5% were achieved.

Limit law of the iterated logarithm for B-valued trimmed sums

Indian Academy of Sciences (India)

Limit law of the iterated logarithm for B-valued trimmed sums. KE-ANG FU1, YUYANG QIU1,∗ and YELING TONG2. 1School of Statistics and Mathematics, Zhejiang Gongshang University,. Hangzhou 310018, China. 2Zhejiang Institute of Traditional Chinese Medicine, Hangzhou 310028, China. *Corresponding author.

Integral Design Methodology of Photocatalytic Reactors for Air Pollution Remediation

Directory of Open Access Journals (Sweden)

Claudio Passalía

2017-06-01

Full Text Available An integral reactor design methodology was developed to address the optimal design of photocatalytic wall reactors to be used in air pollution control. For a target pollutant to be eliminated from an air stream, the proposed methodology is initiated with a mechanistic derived reaction rate. The determination of intrinsic kinetic parameters is associated with the use of a simple geometry laboratory scale reactor, operation under kinetic control and a uniform incident radiation flux, which allows computing the local superficial rate of photon absorption. Thus, a simple model can describe the mass balance and a solution may be obtained. The kinetic parameters may be estimated by the combination of the mathematical model and the experimental results. The validated intrinsic kinetics obtained may be directly used in the scaling-up of any reactor configuration and size. The bench scale reactor may require the use of complex computational software to obtain the fields of velocity, radiation absorption and species concentration. The complete methodology was successfully applied to the elimination of airborne formaldehyde. The kinetic parameters were determined in a flat plate reactor, whilst a bench scale corrugated wall reactor was used to illustrate the scaling-up methodology. In addition, an optimal folding angle of the corrugated reactor was found using computational fluid dynamics tools.

Sample Size Determination for One- and Two-Sample Trimmed Mean Tests

Science.gov (United States)

Luh, Wei-Ming; Olejnik, Stephen; Guo, Jiin-Huarng

2008-01-01

Formulas to determine the necessary sample sizes for parametric tests of group comparisons are available from several sources and appropriate when population distributions are normal. However, in the context of nonnormal population distributions, researchers recommend Yuen's trimmed mean test, but formulas to determine sample sizes have not been…

Top-down design and verification methodology for analog mixed-signal integrated circuits

NARCIS (Netherlands)

Beviz, P.

2016-01-01

The current report contains the introduction of a novel Top-Down Design and Verification methodology for AMS integrated circuits. With the introduction of new design and verification flow, more reliable and efficient development of AMS ICs is possible. The assignment incorporated the research on the

THERP and HEART integrated methodology for human error assessment

Science.gov (United States)

Castiglia, Francesco; Giardina, Mariarosa; Tomarchio, Elio

2015-11-01

THERP and HEART integrated methodology is proposed to investigate accident scenarios that involve operator errors during high-dose-rate (HDR) treatments. The new approach has been modified on the basis of fuzzy set concept with the aim of prioritizing an exhaustive list of erroneous tasks that can lead to patient radiological overexposures. The results allow for the identification of human errors that are necessary to achieve a better understanding of health hazards in the radiotherapy treatment process, so that it can be properly monitored and appropriately managed.

Methodological Bases for Describing Risks of the Enterprise Business Model in Integrated Reporting

Directory of Open Access Journals (Sweden)

Nesterenko Oksana O.

2017-12-01

Full Text Available The aim of the article is to substantiate the methodological bases for describing the business and accounting risks of an enterprise business model in integrated reporting for their timely detection and assessment, and develop methods for their leveling or minimizing and possible prevention. It is proposed to consider risks in the process of forming integrated reporting from two sides: first, risks that arise in the business model of an organization and should be disclosed in its integrated report; second, accounting risks of integrated reporting, which should be taken into account by members of the cross-sectoral working group and management personnel in the process of forming and promulgating integrated reporting. To develop an adequate accounting and analytical tool for disclosure of information about the risks of the business model and integrated reporting, their leveling or minimization, in the article a terminological analysis of the essence of entrepreneurial and accounting risks is carried out. The entrepreneurial risk is defined as an objective-subjective economic category that characterizes the probability of negative or positive consequences of economic-social-ecological activity within the framework of the business model of an enterprise under uncertainty. The accounting risk is suggested to be understood as the probability of unfavorable consequences as a result of organizational, methodological errors in the integrated accounting system, which present threat to the quality, accuracy and reliability of the reporting information on economic, social and environmental activities in integrated reporting as well as threat of inappropriate decision-making by stakeholders based on the integrated report. For the timely identification of business risks and maximum leveling of the influence of accounting risks on the process of formation and publication of integrated reporting, in the study the place of entrepreneurial and accounting risks in

Limit law of the iterated logarithm for B-valued trimmed sums

Indian Academy of Sciences (India)

Annual Meetings · Mid Year Meetings · Discussion Meetings · Public Lectures · Lecture Workshops · Refresher Courses · Symposia · Live Streaming. Home; Journals; Proceedings – Mathematical Sciences; Volume 125; Issue 2. Limit law of the iterated logarithm for -valued trimmed sums. Ke-Ang Fu Yuyang Qiu Yeling ...

Development and validation of the Treatment Related Impact Measure of Weight (TRIM-Weight

Directory of Open Access Journals (Sweden)

Lessard Suzanne

2010-02-01

Full Text Available Abstract Background The use of prescription anti-obesity medication (AOM is becoming increasingly common as treatment options grow and become more accessible. However, AOM may not be without a wide range of potentially negative impacts on patient functioning and well being. The Treatment Related Impact Measure (TRIM-Weight is an obesity treatment-specific patient reported outcomes (PRO measure designed to assess the key impacts of prescription anti-obesity medication. This paper will present the validation findings for the TRIM-Weight. Methods The online validation battery survey was administered in four countries (the U.S., U.K., Australia, and Canada. Eligible subjects were over age eighteen, currently taking a prescription AOM and were currently or had been obese during their life. Validation analyses were conducted according to an a priori statistical analysis plan. Item level psychometric and conceptual criteria were used to refine and reduce the preliminary item pool and factor analysis to identify structural domains was performed. Reliability and validity testing was then performed and the minimally importance difference (MID explored. Results Two hundred and eight subjects completed the survey. Twenty-one of the 43 items were dropped and a five-factor structure was achieved: Daily Life, Weight Management, Treatment Burden, Experience of Side Effects, and Psychological Health. A-priori criteria for internal consistency and test-retest coefficients for the total score and all five subscales were met. All pre-specified hypotheses for convergent and known group validity were also met with the exception of the domain of Daily Life (proven in an ad hoc analysis as well as the 1/2 standard deviation threshold for the MID. Conclusion The development and validation of the TRIM-Weight has been conducted according to well-defined principles for the creation of a PRO measure. Based on the evidence to date, the TRIM-Weight can be considered a brief

Trim24 (Tif1 alpha): an essential 'brake' for retinoic acid-induced transcription to prevent liver cancer.

Science.gov (United States)

Khetchoumian, Konstantin; Teletin, Marius; Tisserand, Johan; Herquel, Benjamin; Ouararhni, Khalid; Losson, Régine

2008-12-01

Retinoic acid (RA), the active derivative of vitamin A, is an important signaling molecule that controls various developmental processes and influence the proliferation and differentiation of a variety of cell types. RA exerts its biological functions primarily through binding to and activating nuclear RA receptors (RARs, which include the RAR alpha, beta and gamma isotypes RARA, RARB and RARC). Aberrant expression or impaired function of these nuclear receptors has been linked to diverse types of cancer. RARs are RA-dependent transcription factors that regulate gene expression through the recruitment of different co-regulators (co-activators and co-repressors). TRIM24 (formerly known as TIF1 alpha) was among the first co-regulators identified as interacting with RARs in a ligand-dependent fashion, and it was recently shown to function in mice as a potent liver-specific tumor suppressor by attenuating Rara-mediated transcription. The fact that Trim24(-/-), but not Trim24(-/-)Rara(+/-), mutant mice are highly predisposed to the development of hepatocellular carcinoma (HCC) has significant implications in cancer research. This result, along with the observation that in response to pharmacological inhibition of the RA signaling, hepatocytes lacking Trim24 loose their ability to proliferate, strongly implicates Rara as a proto-oncogene in hepatocytes and demonstrates that overactivated RA signaling is deleterious to liver homeostasis.

Introducing trimming and function ranking to Solid Works based on function analysis

NARCIS (Netherlands)

Chechurin, Leonid S.; Wits, Wessel Willems; Bakker, Hans M.; Cascini, G.; Vaneker, Thomas H.J.

2011-01-01

TRIZ based Function Analysis models existing products based on functional interactions between product parts. Such a function model description is the ideal starting point for product innovation. Design engineers can apply (TRIZ) methods such as trimming and function ranking to this function model

Introducing Trimming and Function Ranking to SolidWorks based on Function Analysis

NARCIS (Netherlands)

Chechurin, L.S.; Wits, Wessel Willems; Bakker, Hans M.; Vaneker, Thomas H.J.

2015-01-01

TRIZ based Function Analysis models existing products based on functional interactions between product parts. Such a function model description is the ideal starting point for product innovation. Design engineers can apply (TRIZ) methods such as trimming and function ranking to this function model

Integrating FMEA in a Model-Driven Methodology

Science.gov (United States)

Scippacercola, Fabio; Pietrantuono, Roberto; Russo, Stefano; Esper, Alexandre; Silva, Nuno

2016-08-01

Failure Mode and Effects Analysis (FMEA) is a well known technique for evaluating the effects of potential failures of components of a system. FMEA demands for engineering methods and tools able to support the time- consuming tasks of the analyst. We propose to make FMEA part of the design of a critical system, by integration into a model-driven methodology. We show how to conduct the analysis of failure modes, propagation and effects from SysML design models, by means of custom diagrams, which we name FMEA Diagrams. They offer an additional view of the system, tailored to FMEA goals. The enriched model can then be exploited to automatically generate FMEA worksheet and to conduct qualitative and quantitative analyses. We present a case study from a real-world project.

The Formulation of Binding Agent Concentration on Raw Restructured Goat Meat Trimming Product

Directory of Open Access Journals (Sweden)

Rini Mastuti

2012-02-01

Full Text Available The utilization of raw materials and formulation method would affect the quality of the processed meat product. Therefore, a research on the processing method to produce a raw structured product should be conducted. The research was testing a single factor comprises of following parameters: control (without binding agent; 0.3%, 0.5% and 0.7% sodium alginat and 0.3%, 0.5% and 0.7% carragenan, respectively. The results showed that the goat meat trimming could be used as raw materials in the production of the best restructured meat using 0.5% carragenan. This raw restructured meat produced had a loss weight of 1.67%, pH value  7.89, WHC  48.345, and moisture content  75.20%.   Keywords : sodium alginat, carragenan, raw restructured goat meat trimming.

A Methodology to Integrate Security and Cost-effectiveness in ATM

OpenAIRE

Matarese, Francesca; Montefusco, Patrizia; Neves, José; Rocha, André

2014-01-01

The objective of this paper is the definition of a new methodology for carrying out security risk assessment in the air traffic management (ATM) domain so as to enhance security awareness and integrate secure and cost-effective design objectives. This process is carried out by modelling the system, identifying the assets, threats and vulnerabilities, prioritizing the threats and proposing cost-effective countermeasures for the weaknesses found. ATM security is concerned with securing ATM a...

Testing multistage gain and offset trimming in a single photon counting IC with a charge sharing elimination algorithm

International Nuclear Information System (INIS)

Krzyżanowska, A.; Gryboś, P.; Szczygieł, R.; Maj, P.

2015-01-01

Designing a hybrid pixel detector readout electronics operating in a single photon counting mode is a very challenging process, where many main parameters are optimized in parallel (e.g. gain, noise, and threshold dispersion). Additional requirements for a smaller pixel size with extended functionality push designers to use new deep sub-micron technologies. Minimizing the channel size is possible, however, with a decreased pixel size, the charge sharing effect becomes a more important issue. To overcome this problem, we designed an integrated circuit prototype produced in CMOS 40 nm technology, which has an extended functionality of a single pixel. A C8P1 algorithm for the charge sharing effect compensation was implemented. In the algorithm's first stage the charge is rebuilt in a signal rebuilt hub fed by the CSA (charge sensitive amplifier) outputs from four neighbouring pixels. Then, the pixel with the biggest amount of charge is chosen, after a comparison with all the adjacent ones. In order to process the data in such a complicated way, a certain architecture of a single channel was proposed, which allows for: ⋅ processing the signal with the possibility of total charge reconstruction (by connecting with the adjacent pixels), ⋅ a comparison of certain pixel amplitude to its 8 neighbours, ⋅ the extended testability of each block inside the channel to measure CSA gain dispersion, shaper gain dispersion, threshold dispersion (including the simultaneous generation of different pulse amplitudes from different pixels), ⋅ trimming all the necessary blocks for proper operation. We present a solution for multistage gain and offset trimming implemented in the IC prototype. It allows for minimization of the total charge extraction errors, minimization of threshold dispersion in the pixel matrix and minimization of errors of comparison of certain pixel pulse amplitudes with all its neighbours. The detailed architecture of a single channel is presented

Method and apparatus for balancing the magnetic field detecting loops of a cryogenic gradiometer using trimming coils and superconducting disks

International Nuclear Information System (INIS)

Lutes, C.L.

1982-01-01

An apparatus for and a method of measuring the difference in intensity between two coplanar magnetic field vector components at two different points in space. The device is comprised of two interconnected, relatively large, loop patterns of opposite, flux cancelling, winding sense. One or both loops include a trimming element that is itself formed of two interconnected, relatively small, loop patterns of opposite, flux cancelling, winding sense. The device is analyzed for imbalance between the two large loops and is then balanced by placing a balancing superconducting disk of the proper characteristic in or near one of the two small loops of the trimming element. The so-trimmed apparatus forms a gradiometer of substantially improved mensuration

Determining the number of kanbans for dynamic production systems: An integrated methodology

Directory of Open Access Journals (Sweden)

Özlem Uzun Araz

2016-08-01

Full Text Available Just-in-time (JIT is a management philosophy that reduces the inventory levels and eliminates manufacturing wastes by producing only the right quantity at the right time. A kanban system is one of the key elements of JIT philosophy. Kanbans are used to authorize production and to control movement of materials in JIT systems. In Kanban systems, the efficiency of the manufacturing system depends on several factors such as number of kanbans, container size etc. Hence, determining the number of kanbans is a critical decision in Kanban systems. The aim of this study is to develop a methodology that can be used in order to determine the number of kanbans in a dynamic production environment. In this methodology, the changes in system state is monitored in real time manner, and the number of the kanbans are dynamically re-arranged. The proposed methodology integrates simulation, neural networks and Mamdani type fuzzy inference system. The methodology is modelled in simulation environment and applied on a hypothetic production system. We also performed several comparisons for different control policies to show the effectiveness of the proposed methodology.

Methodologies of health impact assessment as part of an integrated approach to reduce effects of air pollution

OpenAIRE

Aunan, Kristin; Seip, Hans Martin

1995-01-01

Quantification of average frequencies of health effects on a population level is an essential part of an integrated assessment of pollution effects. Epidemiological studies seem to provide the best basis for such estimates. This paper gives an introduction to a methodology for health impact assessment. It also gives results from some selected parts of a case-study in Hungary. This study is aimed at testing and improving the methodology for integrated assessment and focuses on energy productio...

Fundamentals of equine podiatry: balanced trimming and physiological shoeing

OpenAIRE

Estrada, Manuel

2014-01-01

The concepts of balanced trimming and physiological shoeing of the equine hoof as a way to prevent asymmetrical weight bearing and related orthopedic problems are discussed in this paper. Suggestions are given for the technical training of the personnel involved.  Considering the quantity and quality of the existing equine herd in Costa Rica, it is recommended to create a Farrier School as well as to coordinate the necessary efforts with other educational institutions related to the animal in...

Integrated Methodology for Estimating Water Use in Mediterranean Agricultural Areas

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George C. Zalidis

2009-08-01

Full Text Available Agricultural use is by far the largest consumer of fresh water worldwide, especially in the Mediterranean, where it has reached unsustainable levels, thus posing a serious threat to water resources. Having a good estimate of the water used in an agricultural area would help water managers create incentives for water savings at the farmer and basin level, and meet the demands of the European Water Framework Directive. This work presents an integrated methodology for estimating water use in Mediterranean agricultural areas. It is based on well established methods of estimating the actual evapotranspiration through surface energy fluxes, customized for better performance under the Mediterranean conditions: small parcel sizes, detailed crop pattern, and lack of necessary data. The methodology has been tested and validated on the agricultural plain of the river Strimonas (Greece using a time series of Terra MODIS and Landsat 5 TM satellite images, and used to produce a seasonal water use map at a high spatial resolution. Finally, a tool has been designed to implement the methodology with a user-friendly interface, in order to facilitate its operational use.

Hypermethylation of TRIM59 and KLF14 Influences Cell Death Signaling in Familial Alzheimer’s Disease

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Michalina Wezyk

2018-01-01

Full Text Available Epigenetic mechanisms play an important role in the development and progression of various neurodegenerative diseases. Abnormal methylation of numerous genes responsible for regulation of transcription, DNA replication, and apoptosis has been linked to Alzheimer’s disease (AD pathology. We have recently performed whole transcriptome profiling of familial early-onset Alzheimer’s disease (fEOAD patient-derived fibroblasts. On this basis, we demonstrated a strong dysregulation of cell cycle checkpoints and DNA damage response (DDR in both fibroblasts and reprogrammed neurons. Here, we show that the aging-correlated hypermethylation of KLF14 and TRIM59 genes associates with abnormalities in DNA repair and cell cycle control in fEOAD. Based on the resulting transcriptome networks, we found that the hypermethylation of KLF14 might be associated with epigenetic regulation of the chromatin organization and mRNA processing followed by hypermethylation of TRIM59 likely associated with the G2/M cell cycle phase and p53 role in DNA repair with BRCA1 protein as the key player. We propose that the hypermethylation of KLF14 could constitute a superior epigenetic mechanism for TRIM59 hypermethylation. The methylation status of both genes affects genome stability and might contribute to proapoptotic signaling in AD. Since this study combines data obtained from various tissues from AD patients, it reinforces the view that the genetic methylation status in the blood may be a valuable predictor of molecular processes occurring in affected tissues. Further research is necessary to define a detailed role of TRIM59 and KLF4 in neurodegeneration of neurons.

Computation of the Distribution of the Fiber-Matrix Interface Cracks in the Edge Trimming of CFRP

Science.gov (United States)

Wang, Fu-ji; Zhang, Bo-yu; Ma, Jian-wei; Bi, Guang-jian; Hu, Hai-bo

2018-04-01

Edge trimming is commonly used to bring the CFRP components to right dimension and shape in aerospace industries. However, various forms of undesirable machining damage occur frequently which will significantly decrease the material performance of CFRP. The damage is difficult to predict and control due to the complicated changing laws, causing unsatisfactory machining quality of CFRP components. Since the most of damage has the same essence: the fiber-matrix interface cracks, this study aims to calculate the distribution of them in edge trimming of CFRP, thereby to obtain the effects of the machining parameters, which could be helpful to guide the optimal selection of the machining parameters in engineering. Through the orthogonal cutting experiments, the quantitative relation between the fiber-matrix interface crack depth and the fiber cutting angle, cutting depth as well as cutting speed is established. According to the analysis on material removal process on any location of the workpiece in edge trimming, the instantaneous cutting parameters are calculated, and the formation process of the fiber-matrix interface crack is revealed. Finally, the computational method for the fiber-matrix interface cracks in edge trimming of CFRP is proposed. Upon the computational results, it is found that the fiber orientations of CFRP workpieces is the most significant factor on the fiber-matrix interface cracks, which can not only change the depth of them from micrometers to millimeters, but control the distribution image of them. Other machining parameters, only influence the fiber-matrix interface cracks depth but have little effect on the distribution image.

New methodology for analytical calculation of resonance integrals in an heterogeneous medium

International Nuclear Information System (INIS)

Campos, T.P.R. de; Martinez, A.S.

1986-01-01

A new methodology for analytical calculation of Resonance Integral in a typical fuel cell is presented. The expression obtained for the Resonance Integral presents the advantage of being analytical. Its constituent terms are combinations of the well known function J(xi,β) with its partial derivatives in regard to β. This is a general expression for all types of resonance. The parameters used in this method depend on the resonance type and are obtained as a function of the parameter lambda. A simple expression, depending on resonance parameters is proposed for this variable. (Author) [pt

Effects of Beak Trimming, Stocking Density and Sex on Carcass Yield, Carcass Components, Plasma Glucose and Triglyceride Levels in Large White Turkeys

Science.gov (United States)

Kiraz, Selahattin

2015-01-01

This study was conducted to determine the effects of beak trimming, stocking density (D) and sex (S) on live weight (LW), carcass yield and its component, and plasma glucose (PG) and triglyceride levels in Large White turkeys. To accomplish this aims, totally 288 d old large white turkey chicks (144 in each sex) were used. Beaks of 77 male and female poults were trimmed when 8 d old with an electrical beak trimmer. The birds were fed by commercial turkey rasion. Experiment was designed as 2 × 2 × 2 factorial arrangement with 3 replications in each group. Beak trimming and stocking density did not affect live weight, carcass composition and its components. The higher LW and carcass weight observed in trimmed groups. As expected, male birds are heavier than female, and carcass percentage (CP) would be adverse. However, in this study, CP of male was higher in trimmed, in 0.25 m2/bird. (D) × sex (S) interaction had an effect on both CP and thigh weights (pcarcass and its some components were higher in male. S × D interaction had an effect on plasma glucose level (p<0.05). Triglyceride level was affected (p<0.05) by sex. Significant relationships were found between percentage of thighs (r=0.447, p<0.01) and percentage of breast (r=0.400, p<0.01). According to this study, it can be said that trimming is useful with density of 0.25 m2/bird in turkey fattening. PMID:26877630

Framework methodology for increased energy efficiency and renewable feedstock integration in industrial clusters

International Nuclear Information System (INIS)

Hackl, Roman; Harvey, Simon

2013-01-01

Highlights: • Framework methodology for energy efficiency of process plants and total sites. • Identification of suitable biorefinery based on host site future energy systems. • Case study results show large energy savings of site wide heat integration. • Case study on refrigeration systems: 15% shaft work savings potential. • Case study on biorefinery integration: utility savings potential of up to 37%. - Abstract: Energy intensive industries, such as the bulk chemical industry, are facing major challenges and adopting strategies to face these challenges. This paper investigates options for clusters of chemical process plants to decrease their energy and emission footprints. There is a wide range of technologies and process integration opportunities available for achieving these objectives, including (i) decreasing fossil fuel and electricity demand by increasing heat integration within individual processes and across the total cluster site; (ii) replacing fossil feedstocks with renewables and biorefinery integration with the existing cluster; (iii) increasing external utilization of excess process heat wherever possible. This paper presents an overview of the use of process integration methods for development of chemical clusters. Process simulation, pinch analysis, Total Site Analysis (TSA) and exergy concepts are combined in a holistic approach to identify opportunities to improve energy efficiency and integrate renewable feedstocks within such clusters. The methodology is illustrated by application to a chemical cluster in Stenungsund on the West Coast of Sweden consisting of five different companies operating six process plants. The paper emphasizes and quantifies the gains that can be made by adopting a total site approach for targeting energy efficiency measures within the cluster and when investigating integration opportunities for advanced biorefinery concepts compared to restricting the analysis to the individual constituent plants. The

Genotyping of TRIM5 locus in northern pig-tailed macaques (Macaca leonina, a primate species susceptible to Human Immunodeficiency Virus type 1 infection

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Jiang Xue-Long

2009-06-01

Full Text Available Abstract Background The pig-tailed macaques are the only Old World monkeys known to be susceptible to human immunodeficiency virus type 1 (HIV-1 infection. We have previously reported that the TRIM5-Cyclophilin A (TRIMCyp fusion in pig-tailed macaques (Macaca nemestrina is dysfunctional in restricting HIV-1, which may explain why pig-tailed macaques are susceptible to HIV-1 infection. Similar results have also been reported by other groups. However, according to the current primate taxonomy, the previously reported M. nemestrina are further classified into three species, which all belong to the Macaca spp. This calls for the need to look into the previous studies in more details. Results The local species Northern pig-tailed macaque (M. leonina was analyzed for the correlation of TRIM5 structure and HIV-1 infection. Eleven M. leonina animals were analyzed, and all of them were found to possess TRIM5-CypA fusion at the TRIM5 locus. The transcripts encoding the dysfunctional TRIM5-CypA should result from the G-to-T mutation in the 3'-splicing site of intron 6. Polymorphism in the putative TRIMCyp recognition domain was observed. The peripheral blood mononuclear cells (PBMCs of M. leonina were susceptible to HIV-1 infection. Consistent with the previous results, expression of the M. leonina TRIMCyp in HeLa-T4 cells rendered the cells resistant to HIV-2ROD but not to SIVmac239 infection. Conclusion The susceptibility of M. leonina to HIV-1 infection is due to the dysfunctional TRIM5-CypA fusion in the TRIM5 locus. This finding should broaden our perspective in developing better HIV/AIDS non-human primate animal models.

Anatomical characterization of hoof growth pattern in six Iranian sheep breeds and its possible implication for trimming recommendations.

Science.gov (United States)

Azarpajouh, S; Marchewka, J; Segura Correa, J C; Calderón Díaz, J A

2018-03-11

The objective of this study was to compare hoof anatomy, hoof growth pattern, and hoof weight-bearing surface of six different Iranian sheep breeds to identify possible differences in the hoof anatomical features that could help to minimize adverse effects of hoof trimming methods. Front and hind hooves of 2-year-old, previously untrimmed, pastured dairy ewes of six Iranian breeds (Afshari, Moghani, Kurdi, Makoui, Chaleshtori, and Lori-Bakhtiari; n = 180 ewes; 30 ewes per breed) were collected after slaughter. Medial and lateral claws were incised sagittally and anatomical measurements such as toe length, heel height, toe height, sole thickness, sole length, and toe angle were recorded in each claw. Data were analyzed using mixed model equations including breed, claw (lateral or medial), hoof (front or hind) and their interactions as fixed effects, and ewe as random effect. Breed differences were observed for all hoof measurements (P < 0.05). Chaleshtori sheep had higher measurements for most of the traits studied while Afshari and Makoui sheep had lower measurements. All measurements, except for toe length and toe height to solar surface to heel height ratio, were significantly greater in the front hooves than in the hind hooves (P < 0.05). Soles were longer in the medial claws compared to the lateral claws of the front hooves (P < 0.05). Results suggest the observed breed differences could interfere with establishing a standard, uniform hoof trimming method for sheep. For instance, it might be possible that while Afshari and Makoui sheep could require more conservative trimming, Chaleshtori sheep could require to be trimmed more. In consequence, hoof trimming methods might need to be adjusted to specific breed characteristics to avoid possible tissue damage.

The IDEAL (Integrated Design and Engineering Analysis Languages) modeling methodology: Capabilities and Applications

Science.gov (United States)

Evers, Ken H.; Bachert, Robert F.

1987-01-01

The IDEAL (Integrated Design and Engineering Analysis Languages) modeling methodology has been formulated and applied over a five-year period. It has proven to be a unique, integrated approach utilizing a top-down, structured technique to define and document the system of interest; a knowledge engineering technique to collect and organize system descriptive information; a rapid prototyping technique to perform preliminary system performance analysis; and a sophisticated simulation technique to perform in-depth system performance analysis.

A solar reserve methodology for renewable energy integration studies based on sub-hourly variability analysis

Energy Technology Data Exchange (ETDEWEB)

Ibanez, Eduardo; Brinkman, Gregory; Hummon, Marissa [National Renewable Energy Lab. (NREL), Golden, CO (United States); Lew, Debra

2012-07-01

Increasing penetration of wind and solar energy are raising concerns among electric system operators because of the variability and uncertainty associated with the power sources. Previous work focused on the quantification of reserves for systems with wind power. This paper presents a new methodology that allows the determination of necessary reserves for high penetrations of photovoltaic power and compares it to the wind-based methodology. The solar reserve methodology was applied to Phase 2 of the Western Wind and Solar Integration Study. A summary of the results is included. (orig.)

Methodology for Selecting Best Management Practices Integrating Multiple Stakeholders and Criteria. Part 1: Methodology

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Mauricio Carvallo Aceves

2016-02-01

Full Text Available The implementation of stormwater Best Management Practices (BMPs could help re-establish the natural hydrological cycle of watersheds after urbanization, with each BMP presenting a different performance across a range of criteria (flood prevention, pollutant removal, etc.. Additionally, conflicting views from the relevant stakeholders may arise, resulting in a complex selection process. This paper proposes a methodology for BMP selection based on the application of multi-criteria decision aid (MCDA methods, integrating multiple stakeholder priorities and BMP combinations. First, in the problem definition, the MCDA methods, relevant criteria and design guidelines are selected. Next, information from the preliminary analysis of the watershed is used to obtain a list of relevant BMPs. The third step comprises the watershed modeling and analysis of the BMP alternatives to obtain performance values across purely objective criteria. Afterwards, a stakeholder analysis based on survey applications is carried out to obtain social performance values and criteria priorities. Then, the MCDA methods are applied to obtain the final BMP rankings. The last step considers the sensitivity analysis and rank comparisons in order to draw the final conclusions and recommendations. Future improvements to the methodology could explore inclusion of multiple objective analysis, and alternative means for obtaining social performance values.

Multicriteria decision methodology for selecting technical alternatives in the Mixed Waste Integrated Program

International Nuclear Information System (INIS)

Ferrada, J.J.; Berry, J.B.

1993-11-01

The US Department of Energy (DOE) Mixed Waste Integrated Program (MWIP) has as one of its tasks the identification of a decision methodology and key decision criteria for the selection methodology. The aim of a multicriteria analysis is to provide an instrument for a systematic evaluation of distinct alternative projects. Determination of this methodology will clarify (1) the factors used to evaluate these alternatives, (2) the evaluator's view of the importance of the factors, and (3) the relative value of each alternative. The selected methodology must consider the Comprehensive Environmental Response Compensation and Liability Act (CERCLA) decision-making criteria for application to the analysis technology subsystems developed by the DOE Office of Technology Development. This report contains a compilation of several decision methodologies developed in various national laboratories, institutions, and universities. The purpose of these methodologies may vary, but the core of the decision attributes are very similar. Six approaches were briefly analyzed; from these six, in addition to recommendations made by the MWIP technical support group leaders and CERCLA, the final decision methodology was extracted. Slight variations are observed in the many methodologies developed by different groups, but most of the analyzed methodologies address similar aspects for the most part. These common aspects were the core of the methodology suggested in this report for use within MWIP for the selection of technologies. The set of criteria compiled and developed for this report have been grouped in five categories: (1) process effectiveness, (2) developmental status, (3) life-cycle cost, (4) implementability, and (5) regulatory compliance

Methodology for optimization of process integration schemes in a biorefinery under uncertainty

International Nuclear Information System (INIS)

Marta Abreu de las Villas (Cuba))" data-affiliation=" (Departamento de Ingeniería Química. Facultad de Química y Farmacia. Universidad Central Marta Abreu de las Villas (Cuba))" >González-Cortés, Meilyn; Marta Abreu de las Villas (Cuba))" data-affiliation=" (Departamento de Ingeniería Química. Facultad de Química y Farmacia. Universidad Central Marta Abreu de las Villas (Cuba))" >Martínez-Martínez, Yenisleidys; Marta Abreu de las Villas (Cuba))" data-affiliation=" (Departamento de Ingeniería Química. Facultad de Química y Farmacia. Universidad Central Marta Abreu de las Villas (Cuba))" >Albernas-Carvajal, Yailet; Marta Abreu de las Villas (Cuba))" data-affiliation=" (Departamento de Ingeniería Química. Facultad de Química y Farmacia. Universidad Central Marta Abreu de las Villas (Cuba))" >Pedraza-Garciga, Julio; Marta Abreu de las Villas (Cuba))" data-affiliation=" (Departamento de Ingeniería Química. Facultad de Química y Farmacia. Universidad Central Marta Abreu de las Villas (Cuba))" >Morales-Zamora, Marlen

2017-01-01

The uncertainty has a great impact in the investment decisions, operability of the plants and in the feasibility of integration opportunities in the chemical processes. This paper, presents the steps to consider the optimization of process investment in the processes integration under conditions of uncertainty. It is shown the potentialities of the biomass cane of sugar for the integration with several plants in a biorefinery scheme for the obtaining chemical products, thermal and electric energy. Among the factories with potentialities for this integration are the pulp and paper and sugar factories and other derivative processes. Theses factories have common resources and also have a variety of products that can be exchange between them so certain products generated in a one of them can be raw matter in another plant. The methodology developed guide to obtaining of feasible investment projects under uncertainty. As objective function was considered the maximization of net profitable value in different scenarios that are generated from the integration scheme. (author)

Severe trimming and enhanced competition of laterals as a tool to delay ripening in Tempranillo vineyards under semiarid conditions

Directory of Open Access Journals (Sweden)

Luis Gonzaga Santesteban

2017-05-01

Significance and impact of the study: this study proves the potentiality of the joint use of trimming and increased irrigation to delay ripening, although it is necessary to analyze the implications the obtained delay has on other quality aspects. The lower anthocyanin and phenolic values observed in trimmed vines were not solely due to delayed ripening, as lower values were observed even when data were compared for a given total soluble solid content.

Mountain Plains Learning Experience Guide: Electrical Wiring. Course: Electrical Wiring Trim-Out.

Science.gov (United States)

Arneson, R.; And Others

One of two individualized courses included in an electrical wiring curriculum, this course covers electrical materials installation for the trim-out stage. The course is comprised of five units: (1) Outlets, (2) Fixtures, (3) Switches, (4) Appliances, and (5) Miscellaneous. Each unit begins with a Unit Learning Experience Guide that gives…

An integrated methodology for process improvement and delivery system visualization at a multidisciplinary cancer center.

Science.gov (United States)

Singprasong, Rachanee; Eldabi, Tillal

2013-01-01

Multidisciplinary cancer centers require an integrated, collaborative, and stream-lined workflow in order to provide high quality of patient care. Due to the complex nature of cancer care and continuing changes to treatment techniques and technologies, it is a constant struggle for centers to obtain a systemic and holistic view of treatment workflow for improving the delivery systems. Project management techniques, Responsibility matrix and a swim-lane activity diagram representing sequence of activities can be combined for data collection, presentation, and evaluation of the patient care. This paper presents this integrated methodology using multidisciplinary meetings and walking the route approach for data collection, integrated responsibility matrix and swim-lane activity diagram with activity time for data representation and 5-why and gap analysis approach for data analysis. This enables collection of right detail of information in a shorter time frame by identifying process flaws and deficiencies while being independent of the nature of the patient's disease or treatment techniques. A case study of a multidisciplinary regional cancer centre is used to illustrate effectiveness of the proposed methodology and demonstrates that the methodology is simple to understand, allowing for minimal training of staff and rapid implementation. © 2011 National Association for Healthcare Quality.

Poly(A-Specific Ribonuclease Mediates 3′-End Trimming of Argonaute2-Cleaved Precursor MicroRNAs

Directory of Open Access Journals (Sweden)

Mayuko Yoda

2013-11-01

Full Text Available MicroRNAs (miRNAs are typically generated as ∼22-nucleotide double-stranded RNAs via the processing of precursor hairpins by the ribonuclease III enzyme Dicer, after which they are loaded into Argonaute (Ago proteins to form an RNA-induced silencing complex (RISC. However, the biogenesis of miR-451, an erythropoietic miRNA conserved in vertebrates, occurs independently of Dicer and instead requires cleavage of the 3′ arm of the pre-miR-451 precursor hairpin by Ago2. The 3′ end of the Ago2-cleaved pre-miR-451 intermediate is then trimmed to the mature length by an unknown nuclease. Here, using a classical chromatographic approach, we identified poly(A-specific ribonuclease (PARN as the enzyme responsible for the 3′–5′ exonucleolytic trimming of Ago2-cleaved pre-miR-451. Surprisingly, our data show that trimming of Ago2-cleaved precursor miRNAs is not essential for target silencing, indicating that RISC is functional with miRNAs longer than the mature length. Our findings define the maturation step in the miRNA biogenesis pathway that depends on Ago2-mediated cleavage.

Effect of laceration and trimming of a tendon on the coefficient of friction along the A2 pulley: an in vitro study on turkey tendon.

Science.gov (United States)

Hajipour, L; Gulihar, A; Dias, J

2010-08-01

We carried out lacerations of 50%, followed by trimming, in ten turkey flexor tendons in vitro and measured the coefficient of friction at the tendon-pulley interface with loads of 200 g and 400 g and in 10 degrees , 30 degrees, 50 degrees and 70 degrees of flexion. Laceration increased the coefficient of friction from 0.12 for the intact tendon to 0.3 at both the test loads. Trimming the laceration reduced the coefficient of friction to 0.2. An exponential increase in the gliding resistance was found at 50 degrees and 70 degrees of flexion (p = 0.02 and p = 0.003, respectively) following trimming compared to that of the intact tendon. We concluded that trimming partially lacerated flexor tendons will reduce the gliding resistance at the tendon-pulley interface, but will lead to fragmentation and triggering of the tendon at higher degrees of flexion and loading. We recommend that higher degrees of flexion be avoided during early post-operative rehabilitation following trimming of a flexor tendon.

An integrated impact assessment and weighting methodology: evaluation of the environmental consequences of computer display technology substitution.

Science.gov (United States)

Zhou, Xiaoying; Schoenung, Julie M

2007-04-01

Computer display technology is currently in a state of transition, as the traditional technology of cathode ray tubes is being replaced by liquid crystal display flat-panel technology. Technology substitution and process innovation require the evaluation of the trade-offs among environmental impact, cost, and engineering performance attributes. General impact assessment methodologies, decision analysis and management tools, and optimization methods commonly used in engineering cannot efficiently address the issues needed for such evaluation. The conventional Life Cycle Assessment (LCA) process often generates results that can be subject to multiple interpretations, although the advantages of the LCA concept and framework obtain wide recognition. In the present work, the LCA concept is integrated with Quality Function Deployment (QFD), a popular industrial quality management tool, which is used as the framework for the development of our integrated model. The problem of weighting is addressed by using pairwise comparison of stakeholder preferences. Thus, this paper presents a new integrated analytical approach, Integrated Industrial Ecology Function Deployment (I2-EFD), to assess the environmental behavior of alternative technologies in correlation with their performance and economic characteristics. Computer display technology is used as the case study to further develop our methodology through the modification and integration of various quality management tools (e.g., process mapping, prioritization matrix) and statistical methods (e.g., multi-attribute analysis, cluster analysis). Life cycle thinking provides the foundation for our methodology, as we utilize a published LCA report, which stopped at the characterization step, as our starting point. Further, we evaluate the validity and feasibility of our methodology by considering uncertainty and conducting sensitivity analysis.

Integrated vehicle-based safety systems light-vehicle field operational test, methodology and results report.

Science.gov (United States)

2010-12-01

"This document presents the methodology and results from the light-vehicle field operational test conducted as part of the Integrated Vehicle-Based Safety Systems program. These findings are the result of analyses performed by the University of Michi...

Evolutionary and Functional Analysis of Old World Primate TRIM5 Reveals the Ancient Emergence of Primate Lentiviruses and Convergent Evolution Targeting a Conserved Capsid Interface.

Directory of Open Access Journals (Sweden)

Kevin R McCarthy

2015-08-01

Full Text Available The widespread distribution of lentiviruses among African primates, and the lack of severe pathogenesis in many of these natural reservoirs, are taken as evidence for long-term co-evolution between the simian immunodeficiency viruses (SIVs and their primate hosts. Evidence for positive selection acting on antiviral restriction factors is consistent with virus-host interactions spanning millions of years of primate evolution. However, many restriction mechanisms are not virus-specific, and selection cannot be unambiguously attributed to any one type of virus. We hypothesized that the restriction factor TRIM5, because of its unique specificity for retrovirus capsids, should accumulate adaptive changes in a virus-specific fashion, and therefore, that phylogenetic reconstruction of TRIM5 evolution in African primates should reveal selection by lentiviruses closely related to modern SIVs. We analyzed complete TRIM5 coding sequences of 22 Old World primates and identified a tightly-spaced cluster of branch-specific adaptions appearing in the Cercopithecinae lineage after divergence from the Colobinae around 16 million years ago. Functional assays of both extant TRIM5 orthologs and reconstructed ancestral TRIM5 proteins revealed that this cluster of adaptations in TRIM5 specifically resulted in the ability to restrict Cercopithecine lentiviruses, but had no effect (positive or negative on restriction of other retroviruses, including lentiviruses of non-Cercopithecine primates. The correlation between lineage-specific adaptations and ability to restrict viruses endemic to the same hosts supports the hypothesis that lentiviruses closely related to modern SIVs were present in Africa and infecting the ancestors of Cercopithecine primates as far back as 16 million years ago, and provides insight into the evolution of TRIM5 specificity.

Application of the integrated safety assessment methodology to the protection of electric systems

International Nuclear Information System (INIS)

Hortal, Javier; Izquierdo, Jose M.

1996-01-01

The generalization of classical techniques for risk assessment incorporating dynamic effects is the main objective of the Integrated Safety Assessment Methodology, as practical implementation of Protection Theory. Transient stability, contingency analysis and protection setpoint verification in electric power systems are particularly appropriate domains of application, since the coupling of reliability and dynamic analysis in the protection assessment process is being increasingly demanded. Suitable techniques for dynamic simulation of sequences of switching events in power systems are derived from the use of quasi-linear equation solution algorithms. The application of the methodology, step by step, is illustrated in a simple but representative example

Trimming of mammalian transcriptional networks using network component analysis

Directory of Open Access Journals (Sweden)

Liao James C

2010-10-01

Full Text Available Abstract Background Network Component Analysis (NCA has been used to deduce the activities of transcription factors (TFs from gene expression data and the TF-gene binding relationship. However, the TF-gene interaction varies in different environmental conditions and tissues, but such information is rarely available and cannot be predicted simply by motif analysis. Thus, it is beneficial to identify key TF-gene interactions under the experimental condition based on transcriptome data. Such information would be useful in identifying key regulatory pathways and gene markers of TFs in further studies. Results We developed an algorithm to trim network connectivity such that the important regulatory interactions between the TFs and the genes were retained and the regulatory signals were deduced. Theoretical studies demonstrated that the regulatory signals were accurately reconstructed even in the case where only three independent transcriptome datasets were available. At least 80% of the main target genes were correctly predicted in the extreme condition of high noise level and small number of datasets. Our algorithm was tested with transcriptome data taken from mice under rapamycin treatment. The initial network topology from the literature contains 70 TFs, 778 genes, and 1423 edges between the TFs and genes. Our method retained 1074 edges (i.e. 75% of the original edge number and identified 17 TFs as being significantly perturbed under the experimental condition. Twelve of these TFs are involved in MAPK signaling or myeloid leukemia pathways defined in the KEGG database, or are known to physically interact with each other. Additionally, four of these TFs, which are Hif1a, Cebpb, Nfkb1, and Atf1, are known targets of rapamycin. Furthermore, the trimmed network was able to predict Eno1 as an important target of Hif1a; this key interaction could not be detected without trimming the regulatory network. Conclusions The advantage of our new algorithm

INTEGRATED METHODOLOGY FOR PRODUCT PLANNING USING MULTI CRITERIA ANALYSIS

Directory of Open Access Journals (Sweden)

Tarun Soota

2016-09-01

Full Text Available Integrated approach to multi-criteria decision problems is proposed using quality function deployment and analytical network process. The objective of the work is to rationalize and improve the method of analyzing and interpreting customer needs and technical requirements. The methodology is used to determine, prioritize engineering requirements based on customer needs for development of best product. Framework allows decision maker to decompose a complex problem in a hierarchical structure to show relationship between objective and criteria. Multi-criteria decision modeling is used for extending the hierarchy process to both dependence and feedback. A case study on bikes is presented for the proposed model.

The influence of fat score and fat trimming on primal cut composition ...

African Journals Online (AJOL)

The objectives of this study were to evaluate the primal cut composition of South African lamb carcasses with different fat scores, and to identify cuts suitable for fat trimming. Sixty grain fed Dorper lambs (rams and ewes) were divided into three groups and slaughtered at 30, 36 and 42 kg. Chilled carcass sides were ...

Genetic parameters for claw disorders and the effect of preselecting cows for trimming

NARCIS (Netherlands)

Spek, van der D.; Arendonk, van J.A.M.; Vallee, A.A.A.; Bovenhuis, H.

2013-01-01

Abstract Claw disorders are important traits relevant to dairy cattle breeding from an economical and welfare point of view. Selection for reduced claw disorders can be based on hoof trimmer records. Typically, not all cows in a herd are trimmed. Our objectives were to estimate heritabilities and

Sensitivity of TRIM projections to management, harvest, yield, and stocking adjustment assumptions.

Science.gov (United States)

Susan J. Alexander

1991-01-01

The Timber Resource Inventory Model (TRIM) was used to make several projections of forest industry timber supply for the Douglas-fir region. The sensitivity of these projections to assumptions about management and yields is discussed. A base run is compared to runs in which yields were altered, stocking adjustment was eliminated, harvest assumptions were changed, and...

Construction and assembling of the trim coils for the Milan superconducting cyclotron

International Nuclear Information System (INIS)

Baccaglioni, G.; Cartegni, G.C.; Fusetti, M.; Gini, L.; Grilli, L.

1986-01-01

This paper presents the main characteristics of the trim coils realized for the heavy ions superconducting cyclotron under construction at the Milan University. The guidelines in the choice of the conductor size, of the insulation and cooling parameters are discussed in some details. The main operations in the coils construction, as winding, impregnation, electrical tests and assembling, are described

Water level management of lakes connected to regulated rivers: An integrated modeling and analytical methodology

Science.gov (United States)

Hu, Tengfei; Mao, Jingqiao; Pan, Shunqi; Dai, Lingquan; Zhang, Peipei; Xu, Diandian; Dai, Huichao

2018-07-01

Reservoir operations significantly alter the hydrological regime of the downstream river and river-connected lake, which has far-reaching impacts on the lake ecosystem. To facilitate the management of lakes connected to regulated rivers, the following information must be provided: (1) the response of lake water levels to reservoir operation schedules in the near future and (2) the importance of different rivers in terms of affecting the water levels in different lake regions of interest. We develop an integrated modeling and analytical methodology for the water level management of such lakes. The data-driven method is used to model the lake level as it has the potential of producing quick and accurate predictions. A new genetic algorithm-based synchronized search is proposed to optimize input variable time lags and data-driven model parameters simultaneously. The methodology also involves the orthogonal design and range analysis for extracting the influence of an individual river from that of all the rivers. The integrated methodology is applied to the second largest freshwater lake in China, the Dongting Lake. The results show that: (1) the antecedent lake levels are of crucial importance for the current lake level prediction; (2) the selected river discharge time lags reflect the spatial heterogeneity of the rivers' impacts on lake level changes; (3) the predicted lake levels are in very good agreement with the observed data (RMSE ≤ 0.091 m; R2 ≥ 0.9986). This study demonstrates the practical potential of the integrated methodology, which can provide both the lake level responses to future dam releases and the relative contributions of different rivers to lake level changes.

QFD: a methodological tool for integration of ergonomics at the design stage.

Science.gov (United States)

Marsot, Jacques

2005-03-01

As a marked increase in the number of musculoskeletal disorders was noted in many industrialized countries and more specifically in companies that require the use of hand tools, the French National Research and Safety Institute launched in 1999 a research program on the topic of integrating ergonomics into hand tool design. After a brief review of the problems of integrating ergonomics at the design stage, the paper shows how the "Quality Function Deployment" method has been applied to the design of a boning knife and it highlights the difficulties encountered. Then, it demonstrates how this method can be a methodological tool geared to greater ergonomics consideration in product design.

Conservatism in methodologies for moderator subcooling sufficiency for fuel channel integrity upon pressure tube and calandria tube contact

Energy Technology Data Exchange (ETDEWEB)

Sun, L., E-mail: LSun@nbpower.com [Point Lepreau Generating Station, Lepreau, NB, (Canada)

2015-07-01

During a postulated large LOCA event in CANDU reactors, the pressure tube may balloon to contact with its surrounding calandria tube to transfer heat to the moderator. To confirm the integrity of the fuel channel in this case, many experiments have been performed in the last three decades. Based on the extant database of the pressure tube/calandria tube (PT/CT) contact, an analytical methodology was developed by Canadian Nuclear Industry to determine the sufficiency of moderator subcooling for fuel channel integrity. At the same time a semi-empirical methodology with an idea of Equivalent Moderator Subcooling (EMS) was also developed to judge the sufficiency of the moderator. In this work, some discussions were made over the two methodologies on their conservatism and it is demonstrated that the analytical approach is over conservative comparing with the EMS methodology. By using the EMS methodology, it is demonstrated that applying glass-peened calandria tubes, the requirement to moderator subcooling can be reduced by 10{sup o}C from that for smooth calandria tubes. (author)

Evaluation of agreement among digital dermatitis scoring methods in the milking parlor, pen, and hoof trimming chute.

Science.gov (United States)

Cramer, G; Winders, T; Solano, L; Kleinschmit, D H

2018-03-01

Digital dermatitis (DD) is the most common infectious foot lesion affecting welfare and productivity of dairy cattle. The key to DD control programs is routine and frequent identification of DD lesions. The objective was to evaluate accuracy of detecting and scoring DD lesions in 3 milking parlor designs and in 3 alternative settings compared with scoring in the hoof trimming chute as reference. A total of 552 cows and 1,104 hind feet from 17 freestall farms were scored by 1 observer in the milking parlor and in 1 other setting: pen, headlocks, or management rail. After being scored in the milking parlor and at least 1 other setting, cows were examined in the hoof trimming chute, considered the gold standard. In every setting, all hind feet were inspected visually using a flashlight and without prior washing of feet. Agreement of the scoring settings was assessed using the 5 M-stage scoring system and a dichotomous absence (M0 or M0/M1) or presence (M1 to M4.1 or M2 to M4.1) system. At trimming chute inspection, 44% of feet had a DD lesion, with estimates of 11, 5, 2, 10, and 16% for M1, M2, M3, M4, and M4.1 lesions, respectively. Apparent DD foot-level prevalence at the milking parlor, pen, management rail, and headlocks was 28, 22, 16, and 22%, respectively. M-stages were less discernible in the pen, management rail, and headlocks (apparent prevalence of M1, M2, M3, and M4.1 was ≤1%) compared with the trimming chute and milking parlor. Agreement beyond chance between any scoring setting and trimming chute scoring ranged from 0.48 to 0.70 for the dichotomous scoring system (absence = M0/M1 vs. presence = M2 to M4.1). Diagnostic test performance varied greatly among DD scoring settings but, in general, it had low sensitivity (93%) for detecting any DD lesion. Agreement and test characteristics were not affected by the type of milking parlor. Although the milking parlor and headlocks were the most reliable settings in which to detect DD, none of the settings were

Glucosidase trimming inhibitors preferentially perturb T cell activation induced by CD2 mAb

NARCIS (Netherlands)

van Kemenade, F. J.; Rotteveel, F. T.; van den Broek, L. A.; Baars, P. A.; van Lier, R. A.; Miedema, F.

1994-01-01

Glycosidase trimming inhibitors may be used to study contribution of N-linked glycan moieties in T cell function. We have studied the effects of castanospermine (Cas), swainsonine (Swain), 1-deoxynojirimycin (dNM), and 1-deoxymannojirimycin (dMM) on T cell activation and differentiation. Our

Methodology of structural integrity assesment of CANDU-6 NPP fuel channels

International Nuclear Information System (INIS)

Radu, Vasile

2004-01-01

The paper describes the methodology of assessing the structural integrity in the CANDU-6 fuel channels making use of alternative methods of evaluation. An evaluation of the structural integrity of a CANDU-6 pressure tube made of Zr-2,5%Nb presenting both sharp and blunted defects is done. These analyses are made in compliance with the Canadian guide 'Pressure Tubes Fitness-for-service', and other two Recognizing procedures internationally adopted: the British procedure R6/Rev.4 and the American procedure API 579. Previously, the data base containing the data on materials property as well as the heat and dynamical loads in normal operation in CANDU-6 pressure tubes was established. Obtaining complete diagrams for structural integrity of pressure tubes with sharp and blunted defects in conditions of normal operation and long-term irradiation is the next step of the methodology to be developed. Modelling sharp and blunted defects on the inner side of pressure tubes, which can occur in normal reactor operating conditions is achieved by means of capabilities of pre-processing of two finite element analysis codes namely FEA-Crack and FEA-Flaw. The second part of the work deals with the analyses by finite element method of the fracture mechanics by means of the FEA-Crack code and with the evaluation of sharp and blunted defects by FAD diagrams in compliance with the British procedure R6/Rev.4. For typical models of blunted defects and thermo-mechanical loads specific to normal operation finite element analyses by FEA-Flaw codes were performed. Then FAD-iDHC diagrams were constructed to evaluate the initiation of the slow cracking under hydrogen/deuterium absorption, the known phenomenon of delayed hydride cracking (DHC)

A changing climate: impacts on human exposures to O3 using an integrated modeling methodology

Science.gov (United States)

Predicting the impacts of changing climate on human exposure to air pollution requires future scenarios that account for changes in ambient pollutant concentrations, population sizes and distributions, and housing stocks. An integrated methodology to model changes in human exposu...

An Implentation Methodology for Integrated Resource Management in Urban Water Planning

Science.gov (United States)

Ebrahimi, G.; Thurm, B.; Klein, D. R.; Öberg, G.

2014-12-01

Urban water management requires innovative and integrative approaches to improve sustainability in cities keeping in touch with science progress. Integrated Resource Management (IRM) is one of these strategies and has been developed to integrate various natural and human resources. However, it is becoming increasingly clear that it is challenging to move from vision to implementation. The aim of this paper is to identify strengths and weaknesses of IRM and analyze if the approach might facilitate implementation of sustainability objectives in the water management field. A literature review was performed on peer-reviewed papers that were identified through Google Scholar search for the term 'Integrated Resource Management'. It was found that IRM has been used in a number of contexts such as urban planning, forestry, and management of waste and livestock. Significant implementation challenges are highlighted in the literature. Based on the lessons learned in many different fields, from forestry to communication sciences, important characteristics of IRM approach were found such as the need for adequate governance and strong leaderships, stakeholder's involvement, the learning process and the critical need of appropriate evaluation criteria. We conclude developing an implementation methodology and presenting several recommendations to implement IRM in urban management. While Integrated Water Resource Management (IWRM) is recognized as a fruitful approach to achieve sustainable water management, this study suggests that a shift toward Integrated Resource Management (IRM) can be beneficial as it is designed to facilitate consideration of the interrelationships between various natural and human resources.

Vedic division methodology for high-speed very large scale integration applications

Directory of Open Access Journals (Sweden)

Prabir Saha

2014-02-01

Full Text Available Transistor level implementation of division methodology using ancient Vedic mathematics is reported in this Letter. The potentiality of the ‘Dhvajanka (on top of the flag’ formula was adopted from Vedic mathematics to implement such type of divider for practical very large scale integration applications. The division methodology was implemented through half of the divisor bit instead of the actual divisor, subtraction and little multiplication. Propagation delay and dynamic power consumption of divider circuitry were minimised significantly by stage reduction through Vedic division methodology. The functionality of the division algorithm was checked and performance parameters like propagation delay and dynamic power consumption were calculated through spice spectre with 90 nm complementary metal oxide semiconductor technology. The propagation delay of the resulted (32 ÷ 16 bit divider circuitry was only ∼300 ns and consumed ∼32.5 mW power for a layout area of 17.39 mm^2. Combination of Boolean arithmetic along with ancient Vedic mathematics, substantial amount of iterations were reduced resulted as ∼47, ∼38, 34% reduction in delay and ∼34, ∼21, ∼18% reduction in power were investigated compared with the mostly used (e.g. digit-recurrence, Newton–Raphson, Goldschmidt architectures.

Methodology for the optimal design of an integrated first and second generation ethanol production plant combined with power cogeneration.

Science.gov (United States)

Bechara, Rami; Gomez, Adrien; Saint-Antonin, Valérie; Schweitzer, Jean-Marc; Maréchal, François

2016-08-01

The application of methodologies for the optimal design of integrated processes has seen increased interest in literature. This article builds on previous works and applies a systematic methodology to an integrated first and second generation ethanol production plant with power cogeneration. The methodology breaks into process simulation, heat integration, thermo-economic evaluation, exergy efficiency vs. capital costs, multi-variable, evolutionary optimization, and process selection via profitability maximization. Optimization generated Pareto solutions with exergy efficiency ranging between 39.2% and 44.4% and capital costs from 210M$ to 390M$. The Net Present Value was positive for only two scenarios and for low efficiency, low hydrolysis points. The minimum cellulosic ethanol selling price was sought to obtain a maximum NPV of zero for high efficiency, high hydrolysis alternatives. The obtained optimal configuration presented maximum exergy efficiency, hydrolyzed bagasse fraction, capital costs and ethanol production rate, and minimum cooling water consumption and power production rate. Copyright © 2016 Elsevier Ltd. All rights reserved.

[Problems of world outlook and methodology of science integration in biological studies].

Science.gov (United States)

Khododova, Iu D

1981-01-01

Problems of worldoutlook and methodology of the natural-science knowledge are considered basing on the analysis of tendencies in the development of the membrane theory of cell processes and the use of principles of biological membrane functioning when solving some scientific and applied problems pertaining to different branches of chemistry and biology. The notion scientific knowledge integration is defined as interpenetration of approaches, methods and ideas of different branches of knowledge and enrichment on this basis of their content resulting in knowledge augmentation in each field taken separately. These processes are accompanied by appearance of new branches of knowledge - sciences "on junction" and their subsequent differentiations. The analysis of some gnoseological situations shows that integration of sciences contributes to coordination and some agreement of thinking styles of different specialists, puts forward keen personality of a scientist demanding, in particular, his high professional mobility. Problems of scientific activity organization are considered, which involve social sciences into the integration processes. The role of philosophy in the integration processes is emphasized.

Osteitis of the apex of the third phalanx following foot trimming in a dairy cow : case report

Directory of Open Access Journals (Sweden)

P.N. Thompson

1998-07-01

Full Text Available An adult Ayrshire cow was presented with bilateral hind-limb lameness 5 weeks after routine claw trimming. Examination under intravenous regional anaesthesia revealed ulceration of the sole at the toe of both the right lateral and left medial claws of the hind limbs. Radiographic examination showed osteolysis and pathological fractures of the 3rd phalanx of both affected claws. Wooden blocks were fixed to the 2 healthy claws and the bone fragments were removed using a different technique for each claw. Full recovery followed within 11 weeks. The aetiological factors and treatment options for this condition, which was considered to be caused primarily by incorrect claw trimming, are discussed.

Investigation Of Adhesion Formation In New Stainless Steel Trim Spring Operated Pressure Relief Valves

Energy Technology Data Exchange (ETDEWEB)

Gross, Robert E. [Savannah River Site (SRS), Aiken, SC (United States); Bukowski, Julia V. [Villanova University, Villanova, PA (United States); Goble, William M. [exida, Sellersville, PA (United States)

2013-04-16

Examination of proof test data for new (not previously installed) stainless steel (SS) trim spring operated pressure relief valves (SOPRV) reveals that adhesions form between the seat and disc in about 46% of all such SOPRV. The forces needed to overcome these adhesions can be sufficiently large to cause the SOPRV to fail its proof test (FPT) prior to installation. Furthermore, a significant percentage of SOPRV which are found to FPT are also found to ''fail to open'' (FTO) meaning they would not relief excess pressure in the event of an overpressure event. The cases where adhesions result in FTO or FPT appear to be confined to SOPRV with diameters < 1 in and set pressures < 150 psig and the FTO are estimated to occur in 0.31% to 2.00% of this subpopulation of SS trim SOPRV. The reliability and safety implications of these finding for end-users who do not perform pre-installation testing of SOPRV are discussed.

A fully integrated 16 channel digitally trimmed pulse shaping amplifier

International Nuclear Information System (INIS)

Hearn, W.E.; Wright, M.E.

1993-11-01

A fully integrated CMOS pulse shaping amplifier has been developed at LBL. All frequency dependent networks are included on the chip. Provision is made for tuning to compensate for process variations. The overall architecture and details of the circuitry are discussed. Test results are presented

MODEL - INTEGRAL METHODOLOGY FOR SUCCESSFUL DESIGNING AND IMPLEMENTING OF TQM SYSTEM IN MACEDONIAN COMPANIES

Directory of Open Access Journals (Sweden)

Elizabeta Mitreva

2011-12-01

Full Text Available The subject of this paper is linked with the valorization of the meaning and the perspectives of Total Quality Management (TQM system design and implementation within the domestic companies and creating a model-methodology for improved performance, efficiency and effectiveness. The research is designed as an attempt to depict the existing condition in the Macedonian companies regarding quality system design and implementation, analysed through 4 polls in the "house of quality" whose top is the ultimate management, and as its bases measurement, evaluation, analyzing and comparison of the quality are used. This "house" is being held by 4 subsystems e.g. internal standardization, methods and techniques for flawless work performance, education and motivation and analyses of the quality costs. The data received from the research and the proposal of the integral methodology for designing and implementing of TQM system are designed in turn to help and present useful directions to all Macedonian companies tending to become "world class" organizations. The basis in the creation of this model is the redesign of the business processes which afterword begins as a new phase of the business performance - continued improvement, rolling of Deming's Quality Circle (Plan-Do-Check-Act. The model-methodology proposed in this paper is integral and universal which means that it is applicable to all companies regardless of the business area.

Design and manufacturing status of trim coils for the Wendelstein 7-X stellarator experiment

Energy Technology Data Exchange (ETDEWEB)

Riße, K., E-mail: konrad.risse@ipp.mpg.de [Max-Planck-Institut für Plasmaphysik, Greifswald (Germany); Rummel, Th.; Freundt, S.; Dudek, A.; Renard, S.; Bykov, V.; Köppen, M. [Max-Planck-Institut für Plasmaphysik, Greifswald (Germany); Langish, S.; Neilson, G.H.; Brown, Th.; Chrzanowski, J.; Mardenfeld, M.; Malinowski, F.; Khodak, A.; Zhao, X. [Princeton Plasma Physics Laboratory, Princeton, NJ (United States); Eksaa, G. [Everson Tesla Inc., Nazareth, PA (United States)

2013-10-15

Highlights: ► The trim coil system will fine tune the main magnetic field during plasma operation by reducing the magnetic field errors. ► The coil design and operational parameters are fixed, the manufacturing is running. ► The coils are equipped with temperature sensors and a voltage tap system to monitor the coil temperature. ► The max. operational deflection is in the order of 4.5 mm; the max. shearing stress across bond planes is of order 16 MPa. ► Special clamps equipped with elastomeric pads allow fixing the coils on the outer cryostat wall. -- Abstract: The stellarator fusion experiment Wendelstein 7-X (W7-X) is currently under construction at the Max-Planck-Institut für Plasmaphysik in Greifswald, Germany. The main magnetic field will be provided by a superconducting magnet system which generates a fivefold toroidal periodic magnetic field. However, unavoidable tolerances can result in small deviations of the magnetic field which disturb the toroidal periodicity. In order to have a tool to influence these field errors five additional normal conducting trim coils were designed to allow fine tuning of the main magnetic field during plasma operation. In the frame of an international cooperation the trim coils will be contributed by the US partners. Princeton Plasma Physics Laboratory has accomplished several tasks to develop the final design ready for manufacturing e.g. detailed manufacturing design for the winding and for the coil connection area. The design work was accompanied by a detailed analysis of resulting forces and moments to prove the design. The manufacturing of the coils is running at Everson Tesla Inc; the first two coils were received at IPP.

New gene evolution in the bonus-TIF1-γ/TRIM33 family impacted the architecture of the vertebrate dorsal-ventral patterning network.

Science.gov (United States)

Wisotzkey, Robert G; Quijano, Janine C; Stinchfield, Michael J; Newfeld, Stuart J

2014-09-01

Uncovering how a new gene acquires its function and understanding how the function of a new gene influences existing genetic networks are important topics in evolutionary biology. Here, we demonstrate nonconservation for the embryonic functions of Drosophila Bonus and its newest vertebrate relative TIF1-γ/TRIM33. We showed previously that TIF1-γ/TRIM33 functions as an ubiquitin ligase for the Smad4 signal transducer and antagonizes the Bone Morphogenetic Protein (BMP) signaling network underlying vertebrate dorsal-ventral axis formation. Here, we show that Bonus functions as an agonist of the Decapentaplegic (Dpp) signaling network underlying dorsal-ventral axis formation in flies. The absence of conservation for the roles of Bonus and TIF1-γ/TRIM33 reveals a shift in the dorsal-ventral patterning networks of flies and mice, systems that were previously considered wholly conserved. The shift occurred when the new gene TIF1-γ/TRIM33 replaced the function of the ubiquitin ligase Nedd4L in the lineage leading to vertebrates. Evidence of this replacement is our demonstration that Nedd4 performs the function of TIF1-γ/TRIM33 in flies during dorsal-ventral axis formation. The replacement allowed vertebrate Nedd4L to acquire novel functions as a ubiquitin ligase of vertebrate-specific Smad proteins. Overall our data reveal that the architecture of the Dpp/BMP dorsal-ventral patterning network continued to evolve in the vertebrate lineage, after separation from flies, via the incorporation of new genes. © The Author 2014. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

Total aircraft flight-control system - Balanced open- and closed-loop control with dynamic trim maps

Science.gov (United States)

Smith, G. A.; Meyer, G.

1979-01-01

The availability of the airborne digital computer has made possible a Total Aircraft Flight Control System (TAFCOS) that uses virtually the complete nonlinear propulsive and aerodynamic data for the aircraft to construct dynamic trim maps that represent an inversion of the aircraft model. The trim maps, in series with the aircraft, provide essentially a linear feed-forward path. Basically, open-loop trajectory control is employed with only a small perturbation feedback signal required to compensate for inaccuracy in the aircraft model and for external disturbances. Simulation results for application to an automatic carrier-landing system are presented. Flight-test results for a STOL aircraft operating automatically over a major portion of its flight regime are presented. The concept promises a more rapid and straightforward design from aerodynamic principles, particularly for highly nonlinear configurations, and requires substantially less digital computer capacity than conventional automatic flight-control system designs.

An integrated approach of composting methodologies for solid waste management

Directory of Open Access Journals (Sweden)

K. Kumaresan

2016-03-01

Full Text Available Organic fraction of solid waste, which upon degradation produces foul smell and generates pathogens, if not properly managed. Composting is not a method of waste disposal but it is a method of waste recycling and used for agricultural purposes. An integrated approach of composting methodology was tested for municipal solid waste management. Solid waste first was composted and after 22 days, was further processed by vermicomposting. Samples were routinely taken for analysis of carbon, nitrogen, moisture content, pH and temperature to determine the quality of composting. Decrease in moisture content to 32.1 %, relative decrease in carbon and nitrogen content were also observed. Among the different types of treatment, municipal solid waste + activated sludge integration showed promising results, followed by vermicomposting municipal solid waste + activated sludge combination, compared to the combinations of dried activated sludge, municipal solid waste + activated sludge semisolid and municipal solid waste + sewage water. Thus, windrow composting followed by vermicomposting gave a better result than other methods. Thus this method would serve as a potential alternative for solid waste management.

An integrated approach of composting methodologies for solid waste management

International Nuclear Information System (INIS)

Kumaresan, K.; Balan, R.; Sridhar, A.; Aravind, J.; Kanmani, P.

2016-01-01

Organic fraction of solid waste, which upon degradation produces foul smell and generates pathogens, if not properly managed. Composting is not a method of waste disposal but it is a method of waste recycling and used for agricultural purposes. An integrated approach of composting methodology was tested for municipal solid waste management. Solid waste first was composted and after 22 days, was further processed by vermicomposting. Samples were routinely taken for analysis of carbon, nitrogen, moisture content, p H and temperature to determine the quality of composting. Decrease in moisture content to 32.1 %, relative decrease in carbon and nitrogen content were also observed. Among the different types of treatment, municipal solid waste + activated sludge integration showed promising results, followed by vermicomposting municipal solid waste + activated sludge combination, compared to the combinations of dried activated sludge, municipal solid waste + activated sludge semisolid and municipal solid waste + sewage water. Thus, windrow composting followed by vermicomposting gave a better result than other methods. Thus this method would serve as a potential alternative for solid waste management.

Two impulse trajectory optimization for the RAE-B orbit trim problem

Science.gov (United States)

Payne, M. H.; Pines, S.; Horsewood, J. L.

1972-01-01

The results are reported of work on an appropriate approach to the solution of the optimum two-impulse transfer problem between orbits of specified inclination. The task included a literature search to identify the current state of the art and a definition of the suggested approach for the specific application of a lunar orbit trim. The applications of the results to the problem are included. The formulation for a computer program developed under this task following a more conventional approach is also included.

Safety studies on Korean fusion DEMO plant using integrated safety assessment methodology

International Nuclear Information System (INIS)

Oh, Kyemin; Kang, Myoung-suk; Heo, Gyunyoung; Kim, Hyoung-chan

2014-01-01

Highlights: •The purpose of this paper is to suggest methodology that can investigate safety issues and provides a case study for Korean fusion DEMO plant. •The concepts of integrated safety assessment methodology (ISAM) that can be applied in addressing regulatory requirements and recognizing safety issues for K-DEMO were emphasized. •Phenomena identification and ranking table (PIRT) was proposed. It can recognize vulnerabilities of systems and identify the gaps in technical areas requiring additional researches. •This work is expected to contribute on the conceptual design of safety features for K-DEMO to design engineers and the guidance for regulatory requirements to licensers. -- Abstract: The purpose of this paper is to suggest methodology that can investigate safety issues and provides a case study for Korean fusion DEMO plant (K-DEMO) as a part of R and D program through the National Fusion Research Institute of Korea. Even though nuclear regulation and licensing framework is well setup due to the operating and design experience of Pressurized Water Reactors (PWRs) since 1970s, the regulatory authority of South Korea has concerns on the challenge of facing new nuclear facilities including K-DEMO due to the differences in systems, materials, and inherent safety feature from conventional PWRs. Even though the follow-up of the ITER license process facilitates to deal with significant safety issues of fusion facilities, a licensee as well as a licenser should identify the gaps between ITER and DEMO in terms of safety issues. First we reviewed the methods of conducting safety analysis for unprecedented nuclear facilities such as Generation IV reactors, particularly very high temperature reactor (VHTR), which is called as integrated safety assessment methodology (ISAM). Second, the analysis for the conceptual design of K-DEMO on the basis of ISAM was conducted. The ISAM consists of five analytical tools to develop the safety requirements from licensee

Safety studies on Korean fusion DEMO plant using integrated safety assessment methodology

Energy Technology Data Exchange (ETDEWEB)

Oh, Kyemin; Kang, Myoung-suk [Kyung Hee University, Youngin-si, Gyeonggi-do 446-701 (Korea, Republic of); Heo, Gyunyoung, E-mail: gheo@khu.ac.kr [Kyung Hee University, Youngin-si, Gyeonggi-do 446-701 (Korea, Republic of); Kim, Hyoung-chan [National Fusion Research Institute, Daejeon-si 305-333 (Korea, Republic of)

2014-10-15

Highlights: •The purpose of this paper is to suggest methodology that can investigate safety issues and provides a case study for Korean fusion DEMO plant. •The concepts of integrated safety assessment methodology (ISAM) that can be applied in addressing regulatory requirements and recognizing safety issues for K-DEMO were emphasized. •Phenomena identification and ranking table (PIRT) was proposed. It can recognize vulnerabilities of systems and identify the gaps in technical areas requiring additional researches. •This work is expected to contribute on the conceptual design of safety features for K-DEMO to design engineers and the guidance for regulatory requirements to licensers. -- Abstract: The purpose of this paper is to suggest methodology that can investigate safety issues and provides a case study for Korean fusion DEMO plant (K-DEMO) as a part of R and D program through the National Fusion Research Institute of Korea. Even though nuclear regulation and licensing framework is well setup due to the operating and design experience of Pressurized Water Reactors (PWRs) since 1970s, the regulatory authority of South Korea has concerns on the challenge of facing new nuclear facilities including K-DEMO due to the differences in systems, materials, and inherent safety feature from conventional PWRs. Even though the follow-up of the ITER license process facilitates to deal with significant safety issues of fusion facilities, a licensee as well as a licenser should identify the gaps between ITER and DEMO in terms of safety issues. First we reviewed the methods of conducting safety analysis for unprecedented nuclear facilities such as Generation IV reactors, particularly very high temperature reactor (VHTR), which is called as integrated safety assessment methodology (ISAM). Second, the analysis for the conceptual design of K-DEMO on the basis of ISAM was conducted. The ISAM consists of five analytical tools to develop the safety requirements from licensee

Usefulness of alternative integrative assessment methodologies in public decision making

Energy Technology Data Exchange (ETDEWEB)

Erickson, L. E.; Litchfield, J. W.; Currie, J. W.; McDonald, C. L.; Adams, R. C.

1978-07-01

Many diverse social, economic, and environmental effects are associated with each of the available energy development alternatives. The assessment of the costs, risks, and benefits of these energy development options is an important function of the U. S. Department of Energy. This task is more difficult when no single alternative is better than the others in all respects. This paper compares benefit-cost and multi-attribute utility analysis as decision aids for these more difficult and more common assessment cases. PNL has developed expertise in making these assessments through its involvement since the Calvert Cliffs decision in both the preparation of Environmental Impact Statements and the development of methods to make these statements more thorough and responsive to the spirit of the National Environmental Protection Act (NEPA). Since 1973 PNL has had continuing efforts to quantify, value, and compare all of the major factors which influence the overall impacts of energy development options. An important part of this work has been the measurement and incorporation of the relative values which community groups place on these conflicting factors. Such difficult assessment problems could be approached in many ways including the use of benefit-cost or multi-attribute utility analysis. This paper addresses the following questions: (1) Should an integrative assessment methodology be used for the overall assessment of these costs, risks, and benefits. (2) If an integrative assessment methodology is to be used, what alternative methods are available and what should be the basis for selecting a method. (3) Is it possible to use one of the available alternatives for one portion of the assessment and another for another portion of the assessment. The answers to these questions presented in this report are applicable to most public decision problems.

Integration of an iterative methodology for exergoeconomic improvement of thermal systems with a process simulator

International Nuclear Information System (INIS)

Vieira, Leonardo S.; Donatelli, Joao L.; Cruz, Manuel E.

2004-01-01

In this paper, we present the development and automated implementation of an iterative methodology for exergoeconomic improvement of thermal systems integrated with a process simulator, so as to be applicable to real, complex plants. The methodology combines recent available exergoeconomic techniques with new qualitative and quantitative criteria for the following tasks: (i) identification of decision variables that affect system total cost and exergetic efficiency; (ii) hierarchical classification of components; (iii) identification of predominant terms in the component total cost; and (iv) choice of main decision variables in the iterative process. To show the strengths and potential advantages of the proposed methodology, it is here applied to the benchmark CGAM cogeneration system. The results obtained are presented and discussed in detail and are compared to those reached using a mathematical optimization procedure

Multicriteria renewable energy planning using an integrated fuzzy VIKOR and AHP methodology: The case of Istanbul

International Nuclear Information System (INIS)

Kaya, Tolga; Kahraman, Cengiz

2010-01-01

The purpose of this study is twofold: first, it is aimed at determining the best renewable energy alternative for Istanbul by using an integrated VIKOR-AHP methodology. Second, a selection among alternative energy production sites in this city is made using the same approach. In the proposed VIKOR-AHP methodology, the weights of the selection criteria are determined by pairwise comparison matrices of AHP. In energy decision making problems, the judgments of decision makers are usually vague. As it is relatively difficult for decision makers to provide exact values for the criteria, the evaluation data for the alternative energy policies should be expressed in linguistic terms. In order to model this kind of uncertainty in human preferences, fuzzy logic is applied very successfully. Thus, both classical VIKOR and classical AHP procedures are performed under fuzzy environment. The originality of the paper comes from the application of the proposed integrated VIKOR-AHP methodology to the selection of the best energy policy and production site. It is found that wind energy is the most appropriate renewable energy option and Catalca district is the best area among the alternatives for establishing wind turbines in Istanbul.

High resolution micro ultrasonic machining for trimming 3D microstructures

International Nuclear Information System (INIS)

Viswanath, Anupam; Li, Tao; Gianchandani, Yogesh

2014-01-01

This paper reports on the evaluation of a high resolution micro ultrasonic machining (HR-µUSM) process suitable for post fabrication trimming of complex 3D microstructures made from fused silica. Unlike conventional USM, the HR-µUSM process aims for low machining rates, providing high resolution and high surface quality. The machining rate is reduced by keeping the micro-tool tip at a fixed distance from the workpiece and vibrating it at a small amplitude. The surface roughness is improved by an appropriate selection of abrasive particles. Fluidic modeling is performed to study interaction among the vibrating micro-tool tip, workpiece, and the slurry. Using 304 stainless steel (SS304) tool tips of 50 µm diameter, the machining performance of the HR-µUSM process is characterized on flat fused silica substrates. The depths and surface finish of machined features are evaluated as functions of slurry concentrations, separation between the micro-tool and workpiece, and machining time. Under the selected conditions, the HR-µUSM process achieves machining rates as low as 10 nm s −1  averaged over the first minute of machining of a flat virgin sample. This corresponds to a mass removal rate of ≈20 ng min −1 . The average surface roughness, S a , achieved is as low as 30 nm. Analytical and numerical modeling are used to explain the typical profile of the machined features as well as machining rates. The process is used to demonstrate trimming of hemispherical 3D shells made of fused silica. (paper)

Effect of irradiation of frozen meat/fat trimmings on microbiological and physicochemical quality attributes of dry fermented sausages.

Science.gov (United States)

Chouliara, I; Samelis, J; Kakouri, A; Badeka, A; Savvaidis, I N; Riganakos, K; Kontominas, M G

2006-10-01

Changes in microbiological and physicochemical quality attributes resulting from the use of irradiation in the production of Greek dry fermented sausage were investigated as a function of fermentation/ripening time. Results showed that irradiating meat/fat trimmings at 2 or 4kGy prior to sausage production eliminated natural contamination with Listeria spp., and reduced pseudomonads, enterococci and pathogenic staphylococci, and enterobacteria, to less than 2 and 1logcfug(-1), respectively. Pseudomonads were very sensitive (>3.4 log reduction) to either radiation dose. Yeasts were the most resistant followed by inherent lactic acid bacteria; their reductions on the trimmings were radiation dose-dependent. Residual effects of irradiation were noted against enterococci, but not against gram-negatives which died off fast during fermentation even in non-irradiated samples. Growth of the starter bacteria, Lactobacillus pentosus and Staphylococcus carnosus, inoculated in the sausage batters post-irradiation was unaffected by the 2 or 4kGy pre-treatment of the trimmings. Irradiation had little or no effect at the end of ripening period (28 days) on pH, moisture content and color (parameters L(∗), a(∗), and b(∗)). Changes in TBA values were small but statistically significant with irradiated samples having higher TBA values than control samples.

Conceptual and methodological challenges to integrating SEA and cumulative effects assessment

International Nuclear Information System (INIS)

Gunn, Jill; Noble, Bram F.

2011-01-01

The constraints to assessing and managing cumulative environmental effects in the context of project-based environmental assessment are well documented, and the potential benefits of a more strategic approach to cumulative effects assessment (CEA) are well argued; however, such benefits have yet to be clearly demonstrated in practice. While it is widely assumed that cumulative effects are best addressed in a strategic context, there has been little investigation as to whether CEA and strategic environmental assessment (SEA) are a 'good fit' - conceptually or methodologically. This paper identifies a number of conceptual and methodological challenges to the integration of CEA and SEA. Based on results of interviews with international experts and practitioners, this paper demonstrates that: definitions and conceptualizations of CEA are typically weak in practice; approaches to effects aggregation vary widely; a systems perspective lacks in both SEA and CEA; the multifarious nature of SEA complicates CEA; tiering arrangements between SEA and project-based assessment are limited to non-existing; and the relationship of SEA to regional planning remains unclear.

A putative SUMO interacting motif in the B30.2/SPRY domain of rhesus macaque TRIM5α important for NF-κB/AP-1 signaling and HIV-1 restriction

Directory of Open Access Journals (Sweden)

Marie-Édith Nepveu-Traversy

2016-01-01

Full Text Available TRIM5α from the rhesus macaque (TRIM5αRh is a restriction factor that shows strong activity against HIV-1. TRIM5αRh binds specifically to HIV-1 capsid (CA through its B30.2/PRYSPRY domain shortly after entry of the virus into the cytoplasm. Recently, three putative SUMO interacting motifs (SIMs have been identified in the PRYSPRY domain of human and macaque TRIM5α. However, structural modeling of this domain suggested that two of them were buried in the hydrophobic core of the protein, implying that interaction with SUMO was implausible, while the third one was not relevant to restriction. In light of these results, we re-analyzed the TRIM5αRh PRYSPRY sequence and identified an additional putative SIM (435VIIC438 which we named SIM4. This motif is exposed at the surface of the PRYSPRY domain, allowing potential interactions with SUMO or SUMOylated proteins. Introducing a double mutation in SIM4 (V435K, I436K did not alter stability, unlike mutations in SIM1. SIM4-mutated TRIM5αRh failed to bind HIV-1CA and lost the ability to restrict this virus. Accordingly, SIM4 undergoes significant variation among primates and substituting this motif with naturally occurring SIM4 variants affected HIV-1 restriction by TRIM5αRh, suggesting a direct role in capsid recognition. Interestingly, SIM4-mutated TRIM5αRh also failed to activate NF-κB and AP-1-mediated transcription. Although there is no direct evidence that SIM4 is involved in direct interaction with SUMO or a SUMOylated protein, mutating this motif strongly reduced co-localization of TRIM5αRh with SUMO-1 and with PML, a SUMOylated nuclear protein. In conclusion, this new putative SIM is crucial for both direct interaction with incoming capsids and for NF-κB/AP-1 signaling. We speculate that the latter function is mediated by interactions of SIM4 with a SUMOylated protein involved in the NF-κB/AP-1 signaling pathways.

A New Integrated Threshold Selection Methodology for Spatial Forecast Verification of Extreme Events

Science.gov (United States)

Kholodovsky, V.

2017-12-01

Extreme weather and climate events such as heavy precipitation, heat waves and strong winds can cause extensive damage to the society in terms of human lives and financial losses. As climate changes, it is important to understand how extreme weather events may change as a result. Climate and statistical models are often independently used to model those phenomena. To better assess performance of the climate models, a variety of spatial forecast verification methods have been developed. However, spatial verification metrics that are widely used in comparing mean states, in most cases, do not have an adequate theoretical justification to benchmark extreme weather events. We proposed a new integrated threshold selection methodology for spatial forecast verification of extreme events that couples existing pattern recognition indices with high threshold choices. This integrated approach has three main steps: 1) dimension reduction; 2) geometric domain mapping; and 3) thresholds clustering. We apply this approach to an observed precipitation dataset over CONUS. The results are evaluated by displaying threshold distribution seasonally, monthly and annually. The method offers user the flexibility of selecting a high threshold that is linked to desired geometrical properties. The proposed high threshold methodology could either complement existing spatial verification methods, where threshold selection is arbitrary, or be directly applicable in extreme value theory.

D Integrated Methodologies for the Documentation and the Virtual Reconstruction of AN Archaeological Site

Science.gov (United States)

Balletti, C.; Guerra, F.; Scocca, V.; Gottardi, C.

2015-02-01

Highly accurate documentation and 3D reconstructions are fundamental for analyses and further interpretations in archaeology. In the last years the integrated digital survey (ground-based survey methods and UAV photogrammetry) has confirmed its main role in the documentation and comprehension of excavation contexts, thanks to instrumental and methodological development concerning the on site data acquisition. The specific aim of the project, reported in this paper and realized by the Laboratory of Photogrammetry of the IUAV University of Venice, is to check different acquisition systems and their effectiveness test, considering each methodology individually or integrated. This research focuses on the awareness that the integration of different survey's methodologies can as a matter of fact increase the representative efficacy of the final representations; these are based on a wider and verified set of georeferenced metric data. Particularly the methods' integration allows reducing or neutralizing issues related to composite and complex objects' survey, since the most appropriate tools and techniques can be chosen considering the characteristics of each part of an archaeological site (i.e. urban structures, architectural monuments, small findings). This paper describes the experience in several sites of the municipality of Sepino (Molise, Italy), where the 3d digital acquisition of cities and structure of monuments, sometimes hard to reach, was realized using active and passive techniques (rage-based and image based methods). This acquisition was planned in order to obtain not only the basic support for interpretation analysis, but also to achieve models of the actual state of conservation of the site on which some reconstructive hypotheses can be based on. Laser scanning data were merged with Structure from Motion techniques' clouds into the same reference system, given by a topographical and GPS survey. These 3d models are not only the final results of the metric

Teaching writing of scientific abstracts in English: CLIL methodology in an integrated English and Medicine course

Directory of Open Access Journals (Sweden)

Philippa Mungra

2010-10-01

Full Text Available In the I Faculty at the University of Rome “La Sapienza” Medical School, one of the several methodology courses aims at developing approaches by physicians to patient queries. One such course comprises several disciplines: Pathology, Immunology, Medical Statistics, Internal Medicine and English, with the specific aim of furnishing students, all Italian speakers, with skills for searching and evaluating the medical literature for answers to patient queries regarding risks and effectiveness of therapy. This paper describes the integration of English into a Methodology Course and delineates how the language component uses a Content and Language Integrated Learning (CLIL approach to train students to write the highly specific genre of journal abstracts for medical experimental research articles. A corpus of student writing is presented and discussed with the aim of furnishing one didactic model for language teaching within the Italian Medical Curriculum.

An integrated methodology to evaluate a spent nuclear fuel storage system

International Nuclear Information System (INIS)

Yoon, Jeong Hyoun

2008-02-01

This study introduced a methodology that can be applied for development of a dry storage system for spent nuclear fuels. It consisted of several design activities that includes development of a simplified program to analyze the amount of spent nuclear fuels from reflecting the practical situation in spent nuclear fuel management and a simplified program to evaluate the cost of 4 types of representing storage system to choose the most competitive option considering economic factor. As verification of the implementation of the reference module to practical purpose, a simplified thermal analysis code was suggested that can see fulfillment of limitation of temperature in long term storage and oxidation analysis. From the thermal related results, the reference module can accommodate full range of PHWR spent nuclear fuels and significant portion of PWR ones too. From the results, the reference storage system can be concluded that has fulfilled the important requirements in terms of long term integrity and radiological safety. Also for the purpose of solving scattered radiation along with deep penetration problems in cooling storage system, small but efficient design alternation was suggested together with its efficiency that can reduce scattered radiation by 1/3 from the original design. Along with the countermeasure for the shielding problem, in consideration of PWR spent nuclear fuels, simplified criticality analysis methodology retaining conservativeness was proposed. The results show the reference module is efficient low enrichment PWR spent nuclear fuel and even relatively high enrichment fuels too if burnup credit is taken. As conclusive remark, the methodology is simple but efficient to plan a concept design of convective cooling type of spent nuclear fuels storage. It can be also concluded that the methodology derived in this study and the reference module has feasibility in practical implementation to mitigate the current complex situation in spent fuel

Design of a Rad-Hard eFuse Trimming Circuit for Bandgap Voltage Reference for LHC Experiments Upgrades

CERN Document Server

Besirli, Mustafa; Koukab, Adil; Michelis, Stefano

A precise and stable reference voltage is required to generate a stable output voltage in DC/DC converters. This reference voltage must be independent of temperature, power supply, radiation, intrinsic technology mismatch and process variation. This master's thesis reports the development of a rad-hard bandgap voltage reference with electrical fuse (eFuse) based analog calibration circuit in a commercial 130nm technology. According to the test results, the maximum error in the bandgap voltage (300mV in this application) was reduced from ±30mV to less than ±0.6mV thanks to the eFuse trimming. A temperature, power supply, radiation, mismatch and process-independent reference voltage was generated to provide reference voltage to first (bPOL12V) and second (bPOL2V5) stage DC/DC converters. This circuit will be integrated in bPOL12V and bPOL2V5 converters for high-luminosity LHC upgrades.

Evaluation of beef trim sampling methods for detection of Shiga toxin-producing Escherichia coli (STEC)

Science.gov (United States)

Presence of Shiga toxin-producing Escherichia coli (STEC) is a major concern in ground beef. Several methods for sampling beef trim prior to grinding are currently used in the beef industry. The purpose of this study was to determine the efficacy of the sampling methods for detecting STEC in beef ...

Validation of an Integrative Methodology to Assess and Monitor Reductive Dechlorination of Chlorinated Ethenes in Contaminated Aquifers

Czech Academy of Sciences Publication Activity Database

Tarnawski, S.E.; Rossi, P.; Brennerová, Mária; Stavělová, M.; Holliger, Ch.

2016-01-01

Roč. 4, February (2016), s. 7 E-ISSN 2296-665X R&D Projects: GA TA ČR TA02020534 Institutional support: RVO:61388971 Keywords : dechlorination * integrative methodology * chlorinated ethenes Subject RIV: EE - Microbiology, Virology

Trends in scenario development methodologies and integration in NUMO's approach

International Nuclear Information System (INIS)

Ebashi, Takeshi; Ishiguro, Katsuhiko; Wakasugi, Keiichiro; Kawamura, Hideki; Gaus, Irina; Vomvoris, Stratis; Martin, Andrew J.; Smith, Paul

2011-01-01

The development of scenarios for quantitative or qualitative analysis is a key element of the assessment of the safety of geological disposal systems. As an outcome of an international workshop attended by European and the Japanese implementers, a number of features common to current methodologies could be identified, as well as trends in their evolution over time. In the late nineties, scenario development was often described as a bottom-up process, whereby scenarios were said to be developed in essence from FEP databases. Nowadays, it is recognised that, in practice, the approaches actually adopted are better described as top-down or 'hybrid', taking as their starting point an integrated (top-down) understanding of the system under consideration including uncertainties in initial state, sometimes assisted by the development of 'storyboards'. A bottom-up element remains (hence the term 'hybrid') to the extent that FEP databases or FEP catalogues (including interactions) are still used, but the focus is generally on completeness checking, which occurs parallel to the main assessment process. Recent advances focus on the consistent treatment of uncertainties throughout the safety assessment and on the integration of operational safety and long term safety. (author)

Value and Vision-based Methodology in Integrated Design

DEFF Research Database (Denmark)

Tollestrup, Christian

on empirical data from workshop where the Value and Vision-based methodology has been taught. The research approach chosen for this investigation is Action Research, where the researcher plays an active role in generating the data and gains a deeper understanding of the investigated phenomena. The result...... of this thesis is the value transformation from an explicit set of values to a product concept using a vision based concept development methodology based on the Pyramid Model (Lerdahl, 2001) in a design team context. The aim of this thesis is to examine how the process of value transformation is occurring within...... is divided in three; the systemic unfolding of the Value and Vision-based methodology, the structured presentation of practical implementation of the methodology and finally the analysis and conclusion regarding the value transformation, phenomena and learning aspects of the methodology....

Methodology for the Integration of Safety in the Optimization of the Advanced Reactors Design

International Nuclear Information System (INIS)

Grinblat, P.; Schlamp, M.; Brasnarof, D.; Gimenez, M.

2003-01-01

In this work a new methodology has been developed and implemented for taking into account the safety levels of the reactor in a design optimization process, by using Design Maps.They represent a new technique for comparing critical variables in case an accidental sequenced happened, with limit values set by the design criteria.So a good balance is achieved, without allowing the economic performance search to cause a too risky reactor, and guaranteeing the competitiveness of it in spite of the safety costs.Up to the moment, there is no design tool able to accomplish this task in an integrated way.A computational tool based on this methodology has been implemented.These tool specially programmed routines allow carrying out the mentioned tasks

Information technology security system engineering methodology

Science.gov (United States)

Childs, D.

2003-01-01

A methodology is described for system engineering security into large information technology systems under development. The methodology is an integration of a risk management process and a generic system development life cycle process. The methodology is to be used by Security System Engineers to effectively engineer and integrate information technology security into a target system as it progresses through the development life cycle. The methodology can also be used to re-engineer security into a legacy system.

Shrinkage covariance matrix approach based on robust trimmed mean in gene sets detection

Science.gov (United States)

Karjanto, Suryaefiza; Ramli, Norazan Mohamed; Ghani, Nor Azura Md; Aripin, Rasimah; Yusop, Noorezatty Mohd

2015-02-01

Microarray involves of placing an orderly arrangement of thousands of gene sequences in a grid on a suitable surface. The technology has made a novelty discovery since its development and obtained an increasing attention among researchers. The widespread of microarray technology is largely due to its ability to perform simultaneous analysis of thousands of genes in a massively parallel manner in one experiment. Hence, it provides valuable knowledge on gene interaction and function. The microarray data set typically consists of tens of thousands of genes (variables) from just dozens of samples due to various constraints. Therefore, the sample covariance matrix in Hotelling's T2 statistic is not positive definite and become singular, thus it cannot be inverted. In this research, the Hotelling's T2 statistic is combined with a shrinkage approach as an alternative estimation to estimate the covariance matrix to detect significant gene sets. The use of shrinkage covariance matrix overcomes the singularity problem by converting an unbiased to an improved biased estimator of covariance matrix. Robust trimmed mean is integrated into the shrinkage matrix to reduce the influence of outliers and consequently increases its efficiency. The performance of the proposed method is measured using several simulation designs. The results are expected to outperform existing techniques in many tested conditions.

Epoxy resin casting of trim coils for superconducting cyclotron

International Nuclear Information System (INIS)

Hajra, D.P.; Sarkar, S.C.; Saha, Subimal; Chaudhuri, J.; Bhandari, R.K.

2006-01-01

The life of any magnet depends on the soundness of the coil insulation, its aging properties and initial and final endurance limitations. The insulation of water-cooled trim coils for superconducting cyclotron is made of glass fibre tape with heat cured unfilled epoxy resin combination. This type of insulation has been selected to achieve excellent stability against thermal and electromagnetic stresses, tight dimensional control, good dielectric strength, non-hygroscopic and considerably low vapour-pressure as it will be inside rough vacuum. The process development and the difficulties encountered for appropriate selection of epoxy resin combination, potting, vacuum process, curing cycle, control of coil dimension to achieve a sound coil absolutely free from cracks, trapped air and voids has been discussed. (author)

The application of life cycle assessment to integrated solid waste management. Pt. 1: Methodology

Energy Technology Data Exchange (ETDEWEB)

Clift, R.; Doig, A.; Finnveden, G.

2000-07-01

Integrated Waste Management is one of the holistic approaches to environmental and resource management which are emerging from applying the concept of sustainable development. Assessment of waste management options requires application of Life Cycle Assessment (LCA). This paper summarizes the methodology for applying LCA to Integrated Waste Management of Municipal Solid Wastes (MSW) developed for and now used by the UK Environment Agency, including recent developments in international fora. Particular attention is devoted to system definition leading to rational and clear compilation of the Life Cycle Inventory, with appropriate 'credit' for recovering materials and/or energy from the waste. LCA of waste management is best seen as a way of structuring information to help decision processes. (Author)

Study of the methodology for sensitivity calculations of fast reactors integral parameters

International Nuclear Information System (INIS)

Renke, C.A.C.

1981-06-01

A study of the methodology for sensitivity calculations of integral parameters of fast reactors for the adjustment of multigroup cross sections is presented. A description of several existent methods and theories is given, with special emphasis being regarded to variational perturbation theory, integrant of the sensitivity code VARI-1D used in this work. Two calculational systems are defined and a set of procedures and criteria is structured gathering the necessary conditions for the determination of the sensitivity coefficients. These coefficients are then computed by both the direct method and the variational perturbation theory. A reasonable number of sensitivity coefficients are computed and analyzed for three fast critical assemblies, covering a range of special interest of the spectrum. These coefficients are determined for severa integral parameters, for the capture and fission cross sections of the U-238 and Pu-239, covering all the energy up to 14.5 MeV. The nuclear data used were obtained the CARNAVAL II calculational system of the Instituto de Engenharia Nuclear. An optimization for sensitivity computations in a chainned sequence of procedures is made, yielding the sensitivities in the energy macrogroups as the final stage. (Author) [pt

Evaluating perceptual integration: uniting response-time- and accuracy-based methodologies.

Science.gov (United States)

Eidels, Ami; Townsend, James T; Hughes, Howard C; Perry, Lacey A

2015-02-01

This investigation brings together a response-time system identification methodology (e.g., Townsend & Wenger Psychonomic Bulletin & Review 11, 391-418, 2004a) and an accuracy methodology, intended to assess models of integration across stimulus dimensions (features, modalities, etc.) that were proposed by Shaw and colleagues (e.g., Mulligan & Shaw Perception & Psychophysics 28, 471-478, 1980). The goal was to theoretically examine these separate strategies and to apply them conjointly to the same set of participants. The empirical phases were carried out within an extension of an established experimental design called the double factorial paradigm (e.g., Townsend & Nozawa Journal of Mathematical Psychology 39, 321-359, 1995). That paradigm, based on response times, permits assessments of architecture (parallel vs. serial processing), stopping rule (exhaustive vs. minimum time), and workload capacity, all within the same blocks of trials. The paradigm introduced by Shaw and colleagues uses a statistic formally analogous to that of the double factorial paradigm, but based on accuracy rather than response times. We demonstrate that the accuracy measure cannot discriminate between parallel and serial processing. Nonetheless, the class of models supported by the accuracy data possesses a suitable interpretation within the same set of models supported by the response-time data. The supported model, consistent across individuals, is parallel and has limited capacity, with the participants employing the appropriate stopping rule for the experimental setting.

Human-animal interactions and safety during dairy cattle handling--Comparing moving cows to milking and hoof trimming.

Science.gov (United States)

Lindahl, C; Pinzke, S; Herlin, A; Keeling, L J

2016-03-01

Cattle handling is a dangerous activity on dairy farms, and cows are a major cause of injuries to livestock handlers. Even if dairy cows are generally tranquil and docile, when situations occur that they perceive or remember as aversive, they may become agitated and hazardous to handle. This study aimed to compare human-animal interactions, cow behavior, and handler safety when moving cows to daily milking and moving cows to more rarely occurring and possibly aversive hoof trimming. These processes were observed on 12 Swedish commercial dairy farms. The study included behavioral observations of handler and cows and cow heart rate recordings, as well as recording frequencies of situations and incidents related to an increased injury risk to the handler. At milking, cows were quite easily moved using few interactions. As expected, the cows showed no behavioral signs of stress, fear, or resistance and their heart rate only rose slightly from the baseline (i.e., the average heart rate during an undisturbed period before handling). Moving cows to hoof trimming involved more forceful and gentle interactions compared with moving cows to milking. Furthermore, the cows showed much higher frequencies of behaviors indicative of aversion and fear (e.g., freezing, balking, and resistance), as well as a higher increase in heart rate. The risk of injury to which handlers were exposed also increased when moving cows to hoof trimming rather than to routine milking. Some interactions (such as forceful tactile interactions with an object and pulling a neck strap or halter) appeared to be related to potentially dangerous incidents where the handler was being kicked, head-butted, or run over by a cow. In conclusion, moving cows to hoof trimming resulted in higher frequencies of behaviors indicating fear, more forceful interactions, and increased injury risks to the handler than moving cows to milking. Improving potentially stressful handling procedures (e.g., by better animal handling

Structural integrity assessments of steam generator tubes using the FAD methodology

Energy Technology Data Exchange (ETDEWEB)

Bergant, Marcos A., E-mail: marcos.bergant@cab.cnea.gov.ar [Gerencia CAREM, Centro Atómico Bariloche (CNEA), Av. Bustillo 9500, San Carlos de Bariloche 8400 (Argentina); Yawny, Alejandro A., E-mail: yawny@cab.cnea.gov.ar [División Física de Metales, Centro Atómico Bariloche (CNEA)/CONICET, Av. Bustillo 9500, San Carlos de Bariloche 8400 (Argentina); Perez Ipiña, Juan E., E-mail: juan.perezipina@fain.uncoma.edu.ar [Grupo Mecánica de Fractura, Universidad Nacional del Comahue/CONICET, Buenos Aires 1400, Neuquén 8300 (Argentina)

2015-12-15

Highlights: • The Failure Assessment Diagram (FAD) is used to assess cracked steam generator tubes. • Typical loading conditions and reported tensile and fracture properties are used. • The FAD is capable to predict the failure mode for different cracks and loads. • The FAD can be used to reduce the conservatism of the current plugging criteria. • Appropriate tensile and fracture properties at operating conditions are required. - Abstract: Steam generator tubes (SGTs) represents up to 60% of the total primary pressure retaining boundary area of a nuclear power plant. They have been found susceptible to diverse degradation mechanisms during service. Due to the significance of a SGT failure on the plant safe operation, nuclear regulatory authorities have established tube plugging or repairing criteria which are based on the defect depth. The widespreadly used “40% criterion” proposed in the 70s is an example whose use is still recommended in the last editions of the ASME Boiler and Pressure Vessel Code. In the present work, an alternative, more realistic and less conservative methodology for SGT integrity evaluation is proposed. It is based on the Failure Assessment Diagram (FAD) and takes advantage of the recent developments in non-destructive techniques which allow a more comprehensive characterization of tube defects, i.e., depth, length, orientation and type. The proposed approach has been applied to: the study of the influence of primary and secondary stresses on tube integrity; the prediction of failure mode (i.e., ductile fracture or plastic collapse) of defective SGTs for varied crack geometries and loading conditions; the analysis of the sensibility of tensile and fracture properties with temperature. The potentiality of the FAD as a comprehensive methodology for predicting the failure loads and failure modes of flawed SGTs is highlighted.

Structural integrity assessments of steam generator tubes using the FAD methodology

International Nuclear Information System (INIS)

Bergant, Marcos A.; Yawny, Alejandro A.; Perez Ipiña, Juan E.

2015-01-01

Highlights: • The Failure Assessment Diagram (FAD) is used to assess cracked steam generator tubes. • Typical loading conditions and reported tensile and fracture properties are used. • The FAD is capable to predict the failure mode for different cracks and loads. • The FAD can be used to reduce the conservatism of the current plugging criteria. • Appropriate tensile and fracture properties at operating conditions are required. - Abstract: Steam generator tubes (SGTs) represents up to 60% of the total primary pressure retaining boundary area of a nuclear power plant. They have been found susceptible to diverse degradation mechanisms during service. Due to the significance of a SGT failure on the plant safe operation, nuclear regulatory authorities have established tube plugging or repairing criteria which are based on the defect depth. The widespreadly used “40% criterion” proposed in the 70s is an example whose use is still recommended in the last editions of the ASME Boiler and Pressure Vessel Code. In the present work, an alternative, more realistic and less conservative methodology for SGT integrity evaluation is proposed. It is based on the Failure Assessment Diagram (FAD) and takes advantage of the recent developments in non-destructive techniques which allow a more comprehensive characterization of tube defects, i.e., depth, length, orientation and type. The proposed approach has been applied to: the study of the influence of primary and secondary stresses on tube integrity; the prediction of failure mode (i.e., ductile fracture or plastic collapse) of defective SGTs for varied crack geometries and loading conditions; the analysis of the sensibility of tensile and fracture properties with temperature. The potentiality of the FAD as a comprehensive methodology for predicting the failure loads and failure modes of flawed SGTs is highlighted.

The Socio-ecological Fit of Human Responses to Environmental Degradation: An Integrated Assessment Methodology

Science.gov (United States)

Briassoulis, Helen

2015-12-01

The scientific and policy interest in the human responses to environmental degradation usually focuses on responses sensu stricto and `best practices' that potentially abate degradation in affected areas. The transfer of individual, discrete instruments and `best practices' to different contexts is challenging, however, because socio-ecological systems are complex and environmental degradation is contextual and contingent. To sensibly assess the effectiveness of formal and informal interventions to combat environmental degradation, the paper proposes an integrative, non-reductionist analytic, the `response assemblage', for the study of `responses-in-context,' i.e., products of human decisions to utilize environmental resources to satisfy human needs in socio-ecological systems. Response assemblages are defined as geographically and historically unique, provisional, open, territorial wholes, complex compositions emerging from processes of assembling biophysical and human components, including responses sensu stricto, from affected focal and other socio-ecological systems, to serve human goals, one of which may be combatting environmental degradation. The degree of match among the components, called the socio- ecological fit of the response assemblage, indicates how effectively their contextual and contingent interactions maintain the socio-ecological resilience, promote sustainable development, and secure the continuous provision of ecosystem services in a focal socio-ecological system. The paper presents a conceptual approach to the analysis of the socio-ecological fit of response assemblages and details an integrated assessment methodology synthesizing the resilience, assemblage, and `problem of fit' literature. Lastly, it summarizes the novelty, value, and policy relevance of conceptualizing human responses as response assemblages and of the integrated assessment methodology, reconsiders `best practices' and suggests selected future research directions.

Application of Decomposition Methodology to Solve Integrated Process Design and Controller Design Problems for Reactor-Separator-Recycle System

DEFF Research Database (Denmark)

Abd.Hamid, Mohd-Kamaruddin; Sin, Gürkan; Gani, Rafiqul

2010-01-01

This paper presents the integrated process design and controller design (IPDC) for a reactor-separator-recycle (RSR) system and evaluates a decomposition methodology to solve the IPDC problem. Accordingly, the IPDC problem is solved by decomposing it into four hierarchical stages: (i) pre...... the design of a RSR system involving consecutive reactions, A B -> C and shown to provide effective solutions that satisfy design, control and cost criteria. The advantage of the proposed methodology is that it is systematic, makes use of thermodynamic-process knowledge and provides valuable insights......-analysis, (ii) design analysis, (iii) controller design analysis, and (iv) final selection and verification. The methodology makes use of thermodynamic-process insights and the reverse design approach to arrive at the final process-controller design decisions. The developed methodology is illustrated through...

Demonstration and Methodology of Structural Monitoring of Stringer Runs out Composite Areas by Embedded Optical Fiber Sensors and Connectors Integrated during Production in a Composite Plant.

Science.gov (United States)

Miguel Giraldo, Carlos; Zúñiga Sagredo, Juan; Sánchez Gómez, José; Corredera, Pedro

2017-07-21

Embedding optical fibers sensors into composite structures for Structural Health Monitoring purposes is not just one of the most attractive solutions contributing to smart structures, but also the optimum integration approach that insures maximum protection and integrity of the fibers. Nevertheless this intended integration level still remains an industrial challenge since today there is no mature integration process in composite plants matching all necessary requirements. This article describes the process developed to integrate optical fiber sensors in the Production cycle of a test specimen. The sensors, Bragg gratings, were integrated into the laminate during automatic tape lay-up and also by a secondary bonding process, both in the Airbus Composite Plant. The test specimen, completely representative of the root joint of the lower wing cover of a real aircraft, is comprised of a structural skin panel with the associated stringer run out. The ingress-egress was achieved through the precise design and integration of miniaturized optical connectors compatible with the manufacturing conditions and operational test requirements. After production, the specimen was trimmed, assembled and bolted to metallic plates to represent the real triform and buttstrap, and eventually installed into the structural test rig. The interrogation of the sensors proves the effectiveness of the integration process; the analysis of the strain results demonstrate the good correlation between fiber sensors and electrical gauges in those locations where they are installed nearby, and the curvature and load transfer analysis in the bolted stringer run out area enable demonstration of the consistency of the fiber sensors measurements. In conclusion, this work presents strong evidence of the performance of embedded optical sensors for structural health monitoring purposes, where in addition and most importantly, the fibers were integrated in a real production environment and the ingress

Heteroscedastic Tests Statistics for One-Way Analysis of Variance: The Trimmed Means and Hall's Transformation Conjunction

Science.gov (United States)

Luh, Wei-Ming; Guo, Jiin-Huarng

2005-01-01

To deal with nonnormal and heterogeneous data for the one-way fixed effect analysis of variance model, the authors adopted a trimmed means method in conjunction with Hall's invertible transformation into a heteroscedastic test statistic (Alexander-Govern test or Welch test). The results of simulation experiments showed that the proposed technique…

Methodological procedures and analytical instruments to evaluate an indicators integrated archive for urban management

International Nuclear Information System (INIS)

Del Ciello, R.; Napoleoni, S.

1998-01-01

This guide provides the results of a research developed at ENEA (National Agency for new Technology, Energy and the Environment) Casaccia center (Rome, Italy) aimed to define methodological procedures and analytical instruments needed to carry out an indicators integrated archive for urban management. The guide also defines the scheme of a negotiation process aimed to reach and exchange data and information among governmental and local administrations, non-governmental organizations and scientific bodies [it

Application of the Integrated Safety Assessment methodology to safety margins. Dynamic Event Trees, Damage Domains and Risk Assessment

International Nuclear Information System (INIS)

Ibánez, L.; Hortal, J.; Queral, C.; Gómez-Magán, J.; Sánchez-Perea, M.; Fernández, I.; Meléndez, E.; Expósito, A.; Izquierdo, J.M.; Gil, J.; Marrao, H.; Villalba-Jabonero, E.

2016-01-01

The Integrated Safety Assessment (ISA) methodology, developed by the Consejo de Seguridad Nuclear, has been applied to an analysis of Zion NPP for sequences with Loss of the Component Cooling Water System (CCWS). The ISA methodology proposal starts from the unfolding of the Dynamic Event Tree (DET). Results from this first step allow assessing the sequence delineation of standard Probabilistic Safety Analysis results. For some sequences of interest of the outlined DET, ISA then identifies the Damage Domain (DD). This is the region of uncertain times and/or parameters where a safety limit is exceeded, which indicates the occurrence of certain damage situation. This paper illustrates application of this concept obtained simulating sequences with MAAP and with TRACE. From information of simulation results of sequence transients belonging to the DD and the time-density probability distributions of the manual actions and of occurrence of stochastic phenomena, ISA integrates the dynamic reliability equations proposed to obtain the sequence contribution to the global Damage Exceedance Frequency (DEF). Reported results show a slight increase in the DEF for sequences investigated following a power uprate from 100% to 110%. This demonstrates the potential use of the method to help in the assessment of design modifications. - Highlights: • This paper illustrates an application of the ISA methodology to safety margins. • Dynamic Event Trees are useful tool for verifying the standard PSA Event Trees. • The ISA methodology takes into account the uncertainties in human action times. • The ISA methodology shows the Damage Exceedance Frequency increase in power uprates.

The association of hoof lesions at claw trimming with test-day milk yield in Danish Holsteins

DEFF Research Database (Denmark)

Ettema, Jehan Frans; Capion, Nynne; Hill, Ashley E.

2007-01-01

on the stage of lactation in which the lesions were diagnosed; during the first 100 days in milk, between days 101 and 200 and between days 201 and 305. Associations between the presence of hoof lesions at trimming and milk production were analyzed by linear mixed modeling at the cow level, clustered within...

Influence of tumbling, tumbling time, trim and sodium tripolyphosphate on quality and yield of cured hams

NARCIS (Netherlands)

Krause, R.J.; Ockerman, H.W.; Krol, B.; Moerman, P.C.; et al.

1978-01-01

Forty‐four boneless, cured hams were assigned to treatment groups to study the effect of tumbling, tumbling time (18 hr intermittent, 9.5 hr intermittent, 3 hr continuous), tumbling temperature (5°C and 15°C), sodium tripolyphosphate (TPP) pickle level (0 and 3.3%), and trim (lean, regular, and fat)

Methodology for Supply Chain Integration: A Case Study in the Artisan Industry of Footwear

OpenAIRE

Pérez-Gallardo, Jorge Raúl; Hernández-Vera, Beatriz; Moras Sánchez, Constantino Gerardo; Aguilar Lasserre, Alberto Alfonso; Posada-Gómez, Rubén; Juárez-Martínez, Ulises; Alor-Hernández, Giner

2014-01-01

The supply chain is a major issue in a global market. The efficient relationship between supplier-producer-retailer conforms the perfect combination to achieve a high level of competitiveness according to the needs of increasingly demanding and changing markets. The difficulty of synchronizing the members within the SC is related to the inherent uncertain factors. This paper proposes a methodology for integrating the supply chain that can be applied in any type of business. To demonstrate its...

The Socio-ecological Fit of Human Responses to Environmental Degradation: An Integrated Assessment Methodology.

Science.gov (United States)

Briassoulis, Helen

2015-12-01

The scientific and policy interest in the human responses to environmental degradation usually focuses on responses sensu stricto and 'best practices' that potentially abate degradation in affected areas. The transfer of individual, discrete instruments and 'best practices' to different contexts is challenging, however, because socio-ecological systems are complex and environmental degradation is contextual and contingent. To sensibly assess the effectiveness of formal and informal interventions to combat environmental degradation, the paper proposes an integrative, non-reductionist analytic, the 'response assemblage', for the study of 'responses-in-context,' i.e., products of human decisions to utilize environmental resources to satisfy human needs in socio-ecological systems. Response assemblages are defined as geographically and historically unique, provisional, open, territorial wholes, complex compositions emerging from processes of assembling biophysical and human components, including responses sensu stricto, from affected focal and other socio-ecological systems, to serve human goals, one of which may be combatting environmental degradation. The degree of match among the components, called the socio-ecological fit of the response assemblage, indicates how effectively their contextual and contingent interactions maintain the socio-ecological resilience, promote sustainable development, and secure the continuous provision of ecosystem services in a focal socio-ecological system. The paper presents a conceptual approach to the analysis of the socio-ecological fit of response assemblages and details an integrated assessment methodology synthesizing the resilience, assemblage, and 'problem of fit' literature. Lastly, it summarizes the novelty, value, and policy relevance of conceptualizing human responses as response assemblages and of the integrated assessment methodology, reconsiders 'best practices' and suggests selected future research directions.

Pemanfaatan trimming kulit pikel sebagai flokulan melalui hidrolisis kolagen menggunakan basa untuk penjernihan air

Directory of Open Access Journals (Sweden)

Sugihartono

2015-07-01

Full Text Available Wastes in the form of skin derivatives from leather tanning industries remain serious problems if not managed properly and quickly, since they can cause environmental pollution. This research aimed at studying the influence of base type and concentration for the hydrolysis of trimmed collagen from pickled sheepskin to the yield and properties of gelatin, as well as the gelatin’s potential as flocculant. The hydrolysis of trimmed collagen from pickled sheepskin was performed using KOH or NaOH solution, each with varied concentrations of 1, 2, 3, and 4% w/v, for 16 hours. The extraction of the resulting gelatin was performed using water (2.5 water part : 1 waste part, at 70-80 oC, for 3 hours. The results show that the yield of gelatin from hydrolysis using KOH (16.50-28.60% is lower than that using NaOH (23.68-34.42%. The water and fat contents of the resulting gelatin were relatively similar, while the protein content from KOH treatment was higher than that from NaOH treatment. Hydrolysis with KOH 1% and NaOH 1% and 2% resulted in gelatin that can be used as flocculant. Hydrolysis with NaOH 2% was the best treatment to produce gelatin as flocculant.

A quantitative flood risk analysis methodology for urban areas with integration of social research data

Science.gov (United States)

Escuder-Bueno, I.; Castillo-Rodríguez, J. T.; Zechner, S.; Jöbstl, C.; Perales-Momparler, S.; Petaccia, G.

2012-09-01

Risk analysis has become a top priority for authorities and stakeholders in many European countries, with the aim of reducing flooding risk, considering the population's needs and improving risk awareness. Within this context, two methodological pieces have been developed in the period 2009-2011 within the SUFRI project (Sustainable Strategies of Urban Flood Risk Management with non-structural measures to cope with the residual risk, 2nd ERA-Net CRUE Funding Initiative). First, the "SUFRI Methodology for pluvial and river flooding risk assessment in urban areas to inform decision-making" provides a comprehensive and quantitative tool for flood risk analysis. Second, the "Methodology for investigation of risk awareness of the population concerned" presents the basis to estimate current risk from a social perspective and identify tendencies in the way floods are understood by citizens. Outcomes of both methods are integrated in this paper with the aim of informing decision making on non-structural protection measures. The results of two case studies are shown to illustrate practical applications of this developed approach. The main advantage of applying the methodology herein presented consists in providing a quantitative estimation of flooding risk before and after investing in non-structural risk mitigation measures. It can be of great interest for decision makers as it provides rational and solid information.

Proposition of a modeling and an analysis methodology of integrated reverse logistics chain in the direct chain

Directory of Open Access Journals (Sweden)

Faycal Mimouni

2016-04-01

Full Text Available Purpose: Propose a modeling and analysis methodology based on the combination of Bayesian networks and Petri networks of the reverse logistics integrated the direct supply chain. Design/methodology/approach: Network modeling by combining Petri and Bayesian network. Findings: Modeling with Bayesian network complimented with Petri network to break the cycle problem in the Bayesian network. Research limitations/implications: Demands are independent from returns. Practical implications: Model can only be used on nonperishable products. Social implications: Legislation aspects: Recycling laws; Protection of environment; Client satisfaction via after sale service. Originality/value: Bayesian network with a cycle combined with the Petri Network.

Efficient running of steam generator trims fuel cost

International Nuclear Information System (INIS)

Selim, M.; Eltouny, S.A.

1993-01-01

E scaling energy prices have led to drastic changes in the operating philosophy of the worldwide industry. About 50% of the thermal energy in industry is being consumed in steam boilers. The new energy reduction programs that have been adopted in egypt draw attention to the boilers, not only to trim energy consumption and improve the production of steam but also to save as much money as possible in doing it. Organization for energy planning (OEP) has started a program for 'Boiler efficiency improvement' in industry since 2 years. The program aimed at performing energy audits in a selective number of industries in both public and private sectors using fire tube boilers produced locally by El Nasr company. As a result of audits an evaluation of performance of this type of boilers was,performed. The energy profiles and the common problems affecting the efficiency of boilers were determined. Energy conservation opportunities (ECO) were identified. 9 figs

Crack Growth-Based Predictive Methodology for the Maintenance of the Structural Integrity of Repaired and Nonrepaired Aging Engine Stationary Components

National Research Council Canada - National Science Library

Barron, Michael

1999-01-01

.... Specifically, the FAA's goal was to develop "Crack Growth-Based Predictive Methodologies for the Maintenance of the Structural Integrity of Repaired and Nonrepaired Aging Engine Stationary Components...

A combined reaction class approach with integrated molecular orbital+molecular orbital (IMOMO) methodology: A practical tool for kinetic modeling

International Nuclear Information System (INIS)

Truong, Thanh N.; Maity, Dilip K.; Truong, Thanh-Thai T.

2000-01-01

We present a new practical computational methodology for predicting thermal rate constants of reactions involving large molecules or a large number of elementary reactions in the same class. This methodology combines the integrated molecular orbital+molecular orbital (IMOMO) approach with our recently proposed reaction class models for tunneling. With the new methodology, we show that it is possible to significantly reduce the computational cost by several orders of magnitude while compromising the accuracy in the predicted rate constants by less than 40% over a wide range of temperatures. Another important result is that the computational cost increases only slightly as the system size increases. (c) 2000 American Institute of Physics

Neonatal Whisker Trimming Impairs Fear/Anxiety-Related Emotional Systems of the Amygdala and Social Behaviors in Adult Mice.

Directory of Open Access Journals (Sweden)

Hitomi Soumiya

Full Text Available Abnormalities in tactile perception, such as sensory defensiveness, are common features in autism spectrum disorder (ASD. While not a diagnostic criterion for ASD, deficits in tactile perception contribute to the observed lack of social communication skills. However, the influence of tactile perception deficits on the development of social behaviors remains uncertain, as do the effects on neuronal circuits related to the emotional regulation of social interactions. In neonatal rodents, whiskers are the most important tactile apparatus, so bilateral whisker trimming is used as a model of early tactile deprivation. To address the influence of tactile deprivation on adult behavior, we performed bilateral whisker trimming in mice for 10 days after birth (BWT10 mice and examined social behaviors, tactile discrimination, and c-Fos expression, a marker of neural activation, in adults after full whisker regrowth. Adult BWT10 mice exhibited significantly shorter crossable distances in the gap-crossing test than age-matched controls, indicating persistent deficits in whisker-dependent tactile perception. In contrast to controls, BWT10 mice exhibited no preference for the social compartment containing a conspecific in the three-chamber test. Furthermore, the development of amygdala circuitry was severely affected in BWT10 mice. Based on the c-Fos expression pattern, hyperactivity was found in BWT10 amygdala circuits for processing fear/anxiety-related responses to height stress but not in circuits for processing reward stimuli during whisker-dependent cued learning. These results demonstrate that neonatal whisker trimming and concomitant whisker-dependent tactile discrimination impairment severely disturbs the development of amygdala-dependent emotional regulation.

Measurements of integrated components' parameters versus irradiation doses gamma radiation (60Co) dosimetry-methodology-tests

International Nuclear Information System (INIS)

Fuan, J.

1991-01-01

This paper describes the methodology used for the irradiation of the integrated components and the measurements of their parameters, using Quality Insurance of dosimetry: - Measurement of the integrated dose using the competences of the Laboratoire Central des Industries Electriques (LCIE): - Measurement of irradiation dose versus source/component distance, using a calibrated equipment. - Use of ALANINE dosimeters, placed on the support of the irradiated components. - Assembly and polarization of components during the irradiations. Selection of the irradiator. - Measurement of the irradiated components's parameters, using the competences of the societies: - GenRad: GR130 tests equipement placed in the DEIN/SIR-CEN SACLAY. - Laboratoire Central des Industries Electriques (LCIE): GR125 tests equipment and this associated programmes test [fr

A novel integrated assessment methodology of urban water reuse.

Science.gov (United States)

Listowski, A; Ngo, H H; Guo, W S; Vigneswaran, S

2011-01-01

Wastewater is no longer considered a waste product and water reuse needs to play a stronger part in securing urban water supply. Although treatment technologies for water reclamation have significantly improved the question that deserves further analysis is, how selection of a particular wastewater treatment technology relates to performance and sustainability? The proposed assessment model integrates; (i) technology, characterised by selected quantity and quality performance parameters; (ii) productivity, efficiency and reliability criteria; (iii) quantitative performance indicators; (iv) development of evaluation model. The challenges related to hierarchy and selections of performance indicators have been resolved through the case study analysis. The goal of this study is to validate a new assessment methodology in relation to performance of the microfiltration (MF) technology, a key element of the treatment process. Specific performance data and measurements were obtained at specific Control and Data Acquisition Points (CP) to satisfy the input-output inventory in relation to water resources, products, material flows, energy requirements, chemicals use, etc. Performance assessment process contains analysis and necessary linking across important parametric functions leading to reliable outcomes and results.

Delamination in surface plies of graphite/epoxy caused by the edge trimming process

Science.gov (United States)

Colligan, K.; Ramulu, M.

Delamination in surface plies of graphite/epoxy laminates caused by edge trimming using polycrystalline diamond (PCD) and carbide cutters is investigated. The effect of several machining variables on formation of delaminations in the surface plies of a graphite fiber reinforced composite material is presented. Machining tests were performed to assess the impact of cutter geometry, feedrate, rotation direction, and graphite fiber orientation. Three typical delamination modes were observed and documented. Feedrate was found to have a significant effect on surface ply delamination in graphite/epoxy composite materials.

Energy Level Composite Curves-a new graphical methodology for the integration of energy intensive processes

International Nuclear Information System (INIS)

Anantharaman, Rahul; Abbas, Own Syed; Gundersen, Truls

2006-01-01

Pinch Analysis, Exergy Analysis and Optimization have all been used independently or in combination for the energy integration of process plants. In order to address the issue of energy integration, taking into account composition and pressure effects, the concept of energy level as proposed by [X. Feng, X.X. Zhu, Combining pinch and exergy analysis for process modifications, Appl. Therm. Eng. 17 (1997) 249] has been modified and expanded in this work. We have developed a strategy for energy integration that uses process simulation tools to define the interaction between the various subsystems in the plant and a graphical technique to help the engineer interpret the results of the simulation with physical insights that point towards exploring possible integration schemes to increase energy efficiency. The proposed graphical representation of energy levels of processes is very similar to the Composite Curves of Pinch Analysis-the interpretation of the Energy Level Composite Curves reduces to the Pinch Analysis case when dealing with heat transfer. Other similarities and differences are detailed in this work. Energy integration of a methanol plant is taken as a case study to test the efficacy of this methodology. Potential integration schemes are identified that would have been difficult to visualize without the help of the new graphical representation

Benefits of using an optimization methodology for identifying robust process integration investments under uncertainty-A pulp mill example

Energy Technology Data Exchange (ETDEWEB)

Svensson, Elin [Department of Energy and Environment, Division of Heat and Power Technology, Chalmers University of Technology, SE-412 96 Goeteborg (Sweden)], E-mail: elin.svensson@chalmers.se; Berntsson, Thore [Department of Energy and Environment, Division of Heat and Power Technology, Chalmers University of Technology, SE-412 96 Goeteborg (Sweden); Stroemberg, Ann-Brith [Fraunhofer-Chalmers Research Centre for Industrial Mathematics, Chalmers Science Park, SE-412 88 Gothenburg (Sweden)

2009-03-15

This paper presents a case study on the optimization of process integration investments in a pulp mill considering uncertainties in future electricity and biofuel prices and CO{sub 2} emissions charges. The work follows the methodology described in Svensson et al. [Svensson, E., Berntsson, T., Stroemberg, A.-B., Patriksson, M., 2008b. An optimization methodology for identifying robust process integration investments under uncertainty. Energy Policy, in press, (doi:10.1016/j.enpol.2008.10.023)] where a scenario-based approach is proposed for the modelling of uncertainties. The results show that the proposed methodology provides a way to handle the time dependence and the uncertainties of the parameters. For the analyzed case, a robust solution is found which turns out to be a combination of two opposing investment strategies. The difference between short-term and strategic views for the investment decision is analyzed and it is found that uncertainties are increasingly important to account for as a more strategic view is employed. Furthermore, the results imply that the obvious effect of policy instruments aimed at decreasing CO{sub 2} emissions is, in applications like this, an increased profitability for all energy efficiency investments, and not as much a shift between different alternatives.

Benefits of using an optimization methodology for identifying robust process integration investments under uncertainty. A pulp mill example

Energy Technology Data Exchange (ETDEWEB)

Svensson, Elin; Berntsson, Thore [Department of Energy and Environment, Division of Heat and Power Technology, Chalmers University of Technology, SE-412 96 Goeteborg (Sweden); Stroemberg, Ann-Brith [Fraunhofer-Chalmers Research Centre for Industrial Mathematics, Chalmers Science Park, SE-412 88 Gothenburg (Sweden)

2009-03-15

This paper presents a case study on the optimization of process integration investments in a pulp mill considering uncertainties in future electricity and biofuel prices and CO{sub 2} emissions charges. The work follows the methodology described in Svensson et al. [Svensson, E., Berntsson, T., Stroemberg, A.-B., Patriksson, M., 2008b. An optimization methodology for identifying robust process integration investments under uncertainty. Energy Policy, in press, doi:10.1016/j.enpol.2008.10.023] where a scenario-based approach is proposed for the modelling of uncertainties. The results show that the proposed methodology provides a way to handle the time dependence and the uncertainties of the parameters. For the analyzed case, a robust solution is found which turns out to be a combination of two opposing investment strategies. The difference between short-term and strategic views for the investment decision is analyzed and it is found that uncertainties are increasingly important to account for as a more strategic view is employed. Furthermore, the results imply that the obvious effect of policy instruments aimed at decreasing CO{sub 2} emissions is, in applications like this, an increased profitability for all energy efficiency investments, and not as much a shift between different alternatives. (author)

Benefits of using an optimization methodology for identifying robust process integration investments under uncertainty-A pulp mill example

International Nuclear Information System (INIS)

Svensson, Elin; Berntsson, Thore; Stroemberg, Ann-Brith

2009-01-01

This paper presents a case study on the optimization of process integration investments in a pulp mill considering uncertainties in future electricity and biofuel prices and CO 2 emissions charges. The work follows the methodology described in Svensson et al. [Svensson, E., Berntsson, T., Stroemberg, A.-B., Patriksson, M., 2008b. An optimization methodology for identifying robust process integration investments under uncertainty. Energy Policy, in press, (doi:10.1016/j.enpol.2008.10.023)] where a scenario-based approach is proposed for the modelling of uncertainties. The results show that the proposed methodology provides a way to handle the time dependence and the uncertainties of the parameters. For the analyzed case, a robust solution is found which turns out to be a combination of two opposing investment strategies. The difference between short-term and strategic views for the investment decision is analyzed and it is found that uncertainties are increasingly important to account for as a more strategic view is employed. Furthermore, the results imply that the obvious effect of policy instruments aimed at decreasing CO 2 emissions is, in applications like this, an increased profitability for all energy efficiency investments, and not as much a shift between different alternatives

Integrated HTA-FMEA/FMECA methodology for the evaluation of robotic system in urology and general surgery.

Science.gov (United States)

Frosini, Francesco; Miniati, Roberto; Grillone, Saverio; Dori, Fabrizio; Gentili, Guido Biffi; Belardinelli, Andrea

2016-11-14

The following study proposes and tests an integrated methodology involving Health Technology Assessment (HTA) and Failure Modes, Effects and Criticality Analysis (FMECA) for the assessment of specific aspects related to robotic surgery involving safety, process and technology. The integrated methodology consists of the application of specific techniques coming from the HTA joined to the aid of the most typical models from reliability engineering such as FMEA/FMECA. The study has also included in-site data collection and interviews to medical personnel. The total number of robotic procedures included in the analysis was 44: 28 for urology and 16 for general surgery. The main outcomes refer to the comparative evaluation between robotic, laparoscopic and open surgery. Risk analysis and mitigation interventions come from FMECA application. The small sample size available for the study represents an important bias, especially for the clinical outcomes reliability. Despite this, the study seems to confirm the better trend for robotics' surgical times with comparison to the open technique as well as confirming the robotics' clinical benefits in urology. More complex situation is observed for general surgery, where robotics' clinical benefits directly measured are the lowest blood transfusion rate.

Trimming the UCERF2 hazard logic tree

Science.gov (United States)

Porter, Keith A.; Field, Edward H.; Milner, Kevin

2012-01-01

The Uniform California Earthquake Rupture Forecast 2 (UCERF2) is a fully time‐dependent earthquake rupture forecast developed with sponsorship of the California Earthquake Authority (Working Group on California Earthquake Probabilities [WGCEP], 2007; Field et al., 2009). UCERF2 contains 480 logic‐tree branches reflecting choices among nine modeling uncertainties in the earthquake rate model shown in Figure 1. For seismic hazard analysis, it is also necessary to choose a ground‐motion‐prediction equation (GMPE) and set its parameters. Choosing among four next‐generation attenuation (NGA) relationships results in a total of 1920 hazard calculations per site. The present work is motivated by a desire to reduce the computational effort involved in a hazard analysis without understating uncertainty. We set out to assess which branching points of the UCERF2 logic tree contribute most to overall uncertainty, and which might be safely ignored (set to only one branch) without significantly biasing results or affecting some useful measure of uncertainty. The trimmed logic tree will have all of the original choices from the branching points that contribute significantly to uncertainty, but only one arbitrarily selected choice from the branching points that do not.

PRODUCT SAFETY AND COLOR CHARACTERISTICS OF GROUND BEEF PROCESSED FROM BEEF TRIMMINGS TREATED WITH PEROXYACETIC ACID ALONE OR FOLLOWED BY NOVEL ORGANIC ACIDS

Directory of Open Access Journals (Sweden)

Fred Pohlman

2014-10-01

Full Text Available The objective of this study was to evaluate the effectiveness of antimicrobial interventions using peroxyacetic acetic acid (PAA followed by novel organic acids on beef trimmings prior to grinding with conventional spray (CS or electrostatic spray (ES on ground beef microbial populations and color. Beef trimmings (80/20; 25kg were inoculated with E. coli O157:H7, non- O157:H7 shiga toxin producing (STEC E. coli (EC and Salmonella spp. (SA at 105 CFU/g. Inoculated trimmings (1.5 kg /treatment/replicate, 2 replicates were treated with CS application of 0.02% PAA alone or followed by CS or ES application of 3% octanoic acid (PO, 3% pyruvic acid (PP, 3% malic acid (PM, saturated solution of fumaric acid (PF or deionized water (W. Findings from this study suggest that PA as a single or multiple chemical hurdle approach with malic, pyruvic, octanoic and fumaric acid on beef trimmings may be effective in reducing E. coli O157:H7 as well as non-STEC serotypes and Salmonella in ground beef up to day 2 of simulated retail display. Results of this study showed that instrumental color properties of ground beef treated with peroxyacetic acid followed by organic acids had little or no difference (P > 0.05 compared to the untreated un-inoculated control ground beef samples. The results also indicate that ES application of some organic acids may have similar or greater efficiency in controlling ground beef microbial populations compared to the CS application of the same acid providing a more economical and waste manageable decontamination approach.

A quantitative flood risk analysis methodology for urban areas with integration of social research data

Directory of Open Access Journals (Sweden)

I. Escuder-Bueno

2012-09-01

Full Text Available Risk analysis has become a top priority for authorities and stakeholders in many European countries, with the aim of reducing flooding risk, considering the population's needs and improving risk awareness. Within this context, two methodological pieces have been developed in the period 2009–2011 within the SUFRI project (Sustainable Strategies of Urban Flood Risk Management with non-structural measures to cope with the residual risk, 2nd ERA-Net CRUE Funding Initiative. First, the "SUFRI Methodology for pluvial and river flooding risk assessment in urban areas to inform decision-making" provides a comprehensive and quantitative tool for flood risk analysis. Second, the "Methodology for investigation of risk awareness of the population concerned" presents the basis to estimate current risk from a social perspective and identify tendencies in the way floods are understood by citizens. Outcomes of both methods are integrated in this paper with the aim of informing decision making on non-structural protection measures. The results of two case studies are shown to illustrate practical applications of this developed approach. The main advantage of applying the methodology herein presented consists in providing a quantitative estimation of flooding risk before and after investing in non-structural risk mitigation measures. It can be of great interest for decision makers as it provides rational and solid information.

Optimization of a High Temperature PEMFC micro-CHP System by Formulation and Application of a Process Integration Methodology

DEFF Research Database (Denmark)

Arsalis, Alexandros; Nielsen, Mads Pagh; Kær, Søren Knudsen

2013-01-01

A 1 kWe micro combined heat and power (CHP) system based on high temperature proton exchange membrane fuel cell (PEMFC) technology is modeled and optimized by formulation and application of a process integration methodology. The system can provide heat and electricity for a singlefamily household...

Distribution and detection of Shiga toxin-producing Escherichia coli (STEC) during an industrial grinding process of beef trim

Science.gov (United States)

During the grinding and packaging processes, it is important to understand how Shiga toxin-producing Escherichia coli (STEC) would be distributed and how well it could be detected in beef trim. This study is important because it shows what would happen if contaminated meat is allowed into a commerc...

Aperture correction with an asymmetrically trimmed gaussian weight in SPECT with a fan-beam collimator

International Nuclear Information System (INIS)

Kamiya, Ryo; Ogawa, Koichi

2013-01-01

The aim of the study is to improve the spatial resolution of single photon emission computed tomography (SPECT) images acquired with a fan-beam collimator. The aperture angle of a hole in the fan-beam collimator depends on the position of the collimator. To correct the aperture effect in an iterative image reconstruction, an asymmetrically trimmed Gaussian weight was used for a model. To confirm the validity of our method, point source phantoms and brain phantom were used in the simulation, and we applied the method to the clinical data. The results of the simulation showed that the spatial resolution of point sources improved from about 6 to 2 pixels full width at half maximum, and the corrected point sources were isotropic. The results of the simulation with the brain phantom showed that our proposed method could improve the spatial resolution of the phantom, and our method was effective for different fan-beam collimators with different focal lengths. The results of clinical data showed that the quality of the reconstructed image was improved with our proposed method. Our proposed aperture correction method with the asymmetrically trimmed Gaussian weighting function was effective in improving the spatial resolution of SPECT images acquired with the fan-beam collimator. (author)

Methodologies of health impact assessment as part of an integrated approach to reduce effects of air pollution

Energy Technology Data Exchange (ETDEWEB)

Aunan, K; Seip, H M

1995-12-01

Quantification of average frequencies of health effects on a population level is an essential part of an integrated assessment of pollution effects. Epidemiological studies seem to provide the best basis for such estimates. This paper gives an introduction to a methodology for health impact assessment and also the results from selected parts of a case study in Hungary. This case study is aimed at testing and improving the methodology for integrated assessment and focuses on energy production and consumption and implications for air pollution. Using monitoring data from Budapest, the paper gives estimates of excess frequencies of respiratory illness, mortality and other health end-points. For a number of health end-points, particles probably may serve as a good indicator component. Stochastic simulation is used to illustrate the uncertainties imbedded in the exposure-response function applied. The paper uses the ``bottom up approach`` to find cost-effective abatement strategies against pollution damages, where specific abatement measures such as emission standards for vehicles are explored in detail. It is concluded that in spite of large uncertainties in every step of the analysis, an integrated assessment of costs and benefits of different abatement measures is valuable as it clarifies the main objectives of an abatement policy and explicitly describes the adverse impacts of different activities and their relative importance. 46 refs., 11 figs., 2 tabs.

A METHODOLOGICAL MODEL FOR INTEGRATING CHARACTER WITHIN CONTENT AND LANGUAGE INTEGRATED LEARNING IN SOCIOLOGY OF RELIGION

Directory of Open Access Journals (Sweden)

Moh Yasir Alimi

2014-02-01

Full Text Available AbstractIn this article, I describe a methodological model I used in a experimental study on how to integrate character within the practice of Content and Language Integrated Learning (CLIL at the higher education Indonesia.This research can be added to research about character education and CLIL in tertiary education, giving nuances to the practice of CLIL so far predominantly a practice in primary and secondary schools.The research was conducted in Semarang State University, in the Department of Sociology and Anthropology, in Sociology of Religion bilingual class. The research indicates that the integration of character within CLIL enrich the perspective of CLIL by strengthening the use of CLIL for intellectual growth and moral development. On the other side, the use of CLIL with character education gives methods and perspectives to the practice of character education which so far only emphasise contents reforms without learning methods reforms. The research also reveals that the weakness of CLIL in using text for classroom learning can be overcome by the use of specific reading and writing strategies. I develop a practical text strategy which can be effectively used in highly conceptual subject such as sociology of religion. AbstrakArtikel ini bertujuan untuk mendeskripsikan model metodologis yang saya pakai untuk mengintegrasikannya karakter dalam Content and Language Integrated Learning (CLIL pada pendidikan tinggi di Indonesia. Penelitian ini memperkaya penelitian mengenai pendidikan karakter dan penerapan CLIL di perguruan tinggi, selama ini penelitian semacam itu hanya biasa di level lebih rendah. Penelitian dilakukan di Universitas Negeri Semarang, pada kelas bilingual yang diikuti 25 mahasiswa, dan diujikan pada mata kuliah Sosiologi Agama. Pelajaran dari penelitian ini adalah integrasi karakter dalam CLIL dapat memperkaya CLIL. Sebaliknya penggunaan CLIL untuk mendidikkan karakter di kelas bilingual mampu menjawab berbagai tantangan

Use of neutron diffraction and laser-induced plasma spectroscopy in integrated authentication methodologies of copper alloy artefacts

International Nuclear Information System (INIS)

Siano, S.; Bartol, L.; Mencaglia, A.A.; Agresti, J.; Miccio, M.

2009-01-01

The present study approaches the general problem of the authentication of copper alloy artefacts of art and historical interest using non-invasive analytical techniques. It aims to demonstrate that a suitable combination of time-of-flight neutron diffraction and laser-induced plasma spectroscopy in integrated multidisciplinary authentication methodologies can provide crucial data for discriminating between genuine archaeological objects and modern counterfeits. After introducing the methodology, which is dedicated in particular to copper alloy figurines of ancient style, two representative authentication case studies are discussed. The results of the work provide evidence that the combination of multiphase analysis using TOF-N D and elemental depth profiles provided by Lips makes it possible to solve most of the present authentication problems.

Disruption of the ASTN2 / TRIM32 locus at 9q33.1 is a risk factor in males for Autism Spectrum Disorders, ADHD and other neurodevelopmental phenotypes

DEFF Research Database (Denmark)

Lionel, Anath C; Tammimies, Kristiina; Vaags, Andrea K

2014-01-01

Rare copy number variants (CNVs) disrupting ASTN2 or both ASTN2 and TRIM32 have been reported at 9q33.1 by genome-wide studies in a few individuals with neurodevelopmental disorders. The vertebrate-specific astrotactins, ASTN2 and its paralog ASTN1, have key roles in glial-guided neuronal migration...... during brain development. To determine the prevalence of astrotactin mutations and delineate their associated phenotypic spectrum, we screened ASTN2/TRIM32 and ASTN1 (1q25.2) for exonic CNVs in clinical microarray data from 89,985 individuals across 10 sites, including 64,114 neurodevelopmental disorder...... subjects. In this clinical dataset, we identified 46 deletions and 12 duplications affecting ASTN2. Deletions of ASTN1 were much rarer. Deletions near the 3' terminus of ASTN2, which would disrupt all transcript isoforms (a subset of these deletions also included TRIM32), were significantly enriched...

Methodologies for Wind Turbine and STATCOM Integration in Wind Power Plant Models for Harmonic Resonances Assessment

DEFF Research Database (Denmark)

Freijedo Fernandez, Francisco Daniel; Chaudhary, Sanjay Kumar; Guerrero, Josep M.

2015-01-01

-domain. As an alternative, a power based averaged modelling is also proposed. Type IV wind turbine harmonic signature and STATCOM active harmonic mitigation are considered for the simulation case studies. Simulation results provide a good insight of the features and limitations of the proposed methodologies.......This paper approaches modelling methodologies for integration of wind turbines and STATCOM in harmonic resonance studies. Firstly, an admittance equivalent model representing the harmonic signature of grid connected voltage source converters is provided. A simplified type IV wind turbine modelling...... is then straightforward. This linear modelling is suitable to represent the wind turbine in the range of frequencies at which harmonic interactions are likely. Even the admittance method is suitable both for frequency and time domain studies, some limitations arise in practice when implementing it in the time...

Probabilistic methodology for turbine missile risk analysis

International Nuclear Information System (INIS)

Twisdale, L.A.; Dunn, W.L.; Frank, R.A.

1984-01-01

A methodology has been developed for estimation of the probabilities of turbine-generated missile damage to nuclear power plant structures and systems. Mathematical models of the missile generation, transport, and impact events have been developed and sequenced to form an integrated turbine missile simulation methodology. Probabilistic Monte Carlo techniques are used to estimate the plant impact and damage probabilities. The methodology has been coded in the TURMIS computer code to facilitate numerical analysis and plant-specific turbine missile probability assessments. Sensitivity analyses have been performed on both the individual models and the integrated methodology, and probabilities have been estimated for a hypothetical nuclear power plant case study. (orig.)

Concerted in vitro trimming of viral HLA-B27-restricted ligands by human ERAP1 and ERAP2 aminopeptidases.

Science.gov (United States)

Lorente, Elena; Barriga, Alejandro; Johnstone, Carolina; Mir, Carmen; Jiménez, Mercedes; López, Daniel

2013-01-01

In the classical human leukocyte antigen (HLA) class I antigen processing and presentation pathway, the antigenic peptides are generated from viral proteins by multiple proteolytic cleavages of the proteasome (and in some cases other cytosolic proteases) and transported to the endoplasmic reticulum (ER) lumen where they are exposed to aminopeptidase activity. In human cells, two different ER-resident enzymes, ERAP1 and ERAP2, can trim the N-terminally extended residues of peptide precursors. In this study, the possible cooperative effect of generating five naturally processed HLA-B27 ligands by both proteases was analyzed. We identified differences in the products obtained with increased detection of natural HLA-B27 ligands by comparing double versus single enzyme digestions by mass spectrometry analysis. These in vitro data suggest that each enzyme can use the degradation products of the other as a substrate for new N-terminal trimming, indicating concerted aminoproteolytic activity of ERAP 1 and ERAP2.

Concerted in vitro trimming of viral HLA-B27-restricted ligands by human ERAP1 and ERAP2 aminopeptidases.

Directory of Open Access Journals (Sweden)

Elena Lorente

Full Text Available In the classical human leukocyte antigen (HLA class I antigen processing and presentation pathway, the antigenic peptides are generated from viral proteins by multiple proteolytic cleavages of the proteasome (and in some cases other cytosolic proteases and transported to the endoplasmic reticulum (ER lumen where they are exposed to aminopeptidase activity. In human cells, two different ER-resident enzymes, ERAP1 and ERAP2, can trim the N-terminally extended residues of peptide precursors. In this study, the possible cooperative effect of generating five naturally processed HLA-B27 ligands by both proteases was analyzed. We identified differences in the products obtained with increased detection of natural HLA-B27 ligands by comparing double versus single enzyme digestions by mass spectrometry analysis. These in vitro data suggest that each enzyme can use the degradation products of the other as a substrate for new N-terminal trimming, indicating concerted aminoproteolytic activity of ERAP 1 and ERAP2.

Characteristics of a continuous denitration by formic acid - electrolytic trimming of residual acid with accompanying the precipitation of metal ions

International Nuclear Information System (INIS)

Kim, G. W.; Kim, S. H.; Lim, J. G.; Lee, I. H.

2003-01-01

This work has studied the characteristics of destruction of nitric acid and precipitation of several metal ions in a continuous denitration process combining a denitration by formic acid and a residual acid-electrolytic trimming system. The metal ions of Zr, Mo, Fe, and Nd did not affect the electrodes at the step of electrolytic trimming of the residual acid after denitration by formic acid. The Mo ion in electrolytic solution enhanced the generation of nitrite ion during the electrolytic reaction. The mole ratio of formic acid to nitric acid fed into the continuous denitration reactor using formic acid affected much the final acidity, the precipitation yields of metal ions, the precipitate morphology. At the ratio of 1.65, the process had the lowest final acidity of less than 0.1 M, and the precipitation yields of Zr and Mo reached 95% and 83%, respectively as the highest values

Integrating environmental restoration management within DOE using an alternative identification and evaluation procedure: A methodology and a case history

International Nuclear Information System (INIS)

Devaurs, M.; Brooks, D.; Kelly, E.; Wagner, S.; Vocke, R.

1992-01-01

The process of identifying and evaluating alternative corrective measures is a fundamental integrating part of Environmental Restoration (ER) activities. The process used in the Los Alamos National Laboratory (the Laboratory) ER Program is based on principles and tools from multiattribute decision analysis, a well-developed and proven method for evaluating options in decision situations involving multiple objectives, uncertainty, multiple interested stakeholders in the final decision, and the need for technical input from disparate disciplines. The process provides a methodology that has been extensively developed and reviewed over the past five decades; it provides a methodological structure for incorporating the concepts espoused in the streamlined approach as well as more specific guidelines such as data quality objectives (DQOs). The application of this methodology to the ER Program at Los Alamos is described in this paper

Vocalist - an international programme for the validation of constraint based methodology in structural integrity

International Nuclear Information System (INIS)

Lidbury, D.; Bass, R.; Gilles, Ph.; Connors, D.; Eisele, U.; Keim, E.; Keinanen, H.; Marie, St.; Nagel, G.; Taylor, N.; Wadier, Y.

2001-01-01

The pattern of crack-tip stresses and strains causing plastic flow and fracture in components is different to that in test specimens. This gives rise to the so-called constraint effect. Crack-tip constraint in components is generally lower than in test specimens. Effective toughness is correspondingly higher. The fracture toughness measured on test specimens is thus likely to underestimate that exhibited by cracks in components. A 36-month programme was initiated in October 2000 as part of the Fifth Framework of the European Atomic Energy Community (EURATOM), with the objective of achieving (i) an improved defect assessment methodology for predicting safety margins; (ii) improved lifetime management arguments. The programme VOCALIST (Validation of Constraint Based Methodology in Structural Integrity) is one of a 'cluster' of Fifth Framework projects in the area of Plant Life Management (Nuclear Fission). VOCALIST is also an associated project of NESC (Network for Evaluating Steel Components). The present paper describes the aims and objectives of VOCALIST, its interactions with NESC, and gives details of its various Work Packages. (authors)

Vocalist - an international programme for the validation of constraint based methodology in structural integrity

Energy Technology Data Exchange (ETDEWEB)

Lidbury, D. [AEA Technology, Consulting (United Kingdom); Bass, R. [Oak Ridge National Lab., TN (United States); Gilles, Ph. [FRAMATOME, 92 - Paris-La-Defence (France); Connors, D. [BNFL Magnox Generation (United Kingdom); Eisele, U. [Multiphoton Absorption, MPA, Stuttgart (Germany); Keim, E. [Framatome ANP GmbH (Germany); Keinanen, H. [VTT Energy, Espoo (Finland); Marie, St. [CEA Saclay, Dept. de Mecanique et de Technologie, 91 - Gif sur Yvette (France); Nagel, G. [E.ON Kernraft (Germany); Taylor, N. [Joint Research Center, JRC-IAM (Netherlands); Wadier, Y. [Electricite de France (EDF), 93 - Saint-Denis (France). Dept. de Radioprotection

2001-07-01

The pattern of crack-tip stresses and strains causing plastic flow and fracture in components is different to that in test specimens. This gives rise to the so-called constraint effect. Crack-tip constraint in components is generally lower than in test specimens. Effective toughness is correspondingly higher. The fracture toughness measured on test specimens is thus likely to underestimate that exhibited by cracks in components. A 36-month programme was initiated in October 2000 as part of the Fifth Framework of the European Atomic Energy Community (EURATOM), with the objective of achieving (i) an improved defect assessment methodology for predicting safety margins; (ii) improved lifetime management arguments. The programme VOCALIST (Validation of Constraint Based Methodology in Structural Integrity) is one of a 'cluster' of Fifth Framework projects in the area of Plant Life Management (Nuclear Fission). VOCALIST is also an associated project of NESC (Network for Evaluating Steel Components). The present paper describes the aims and objectives of VOCALIST, its interactions with NESC, and gives details of its various Work Packages. (authors)

Analysis of cold leg LOCA with failed HPSI by means of integrated safety assessment methodology

International Nuclear Information System (INIS)

Gonzalez-Cadelo, J.; Queral, C.; Montero-Mayorga, J.

2014-01-01

Highlights: • Results of ISA for considered sequences endorse EOPs guidance in an original way. • ISA allows to obtain accurate available times for accident management actions. • RCP-trip adequacy and available time for beginning depressurization are evaluated. • ISA minimizes the necessity of expert judgment to perform safety assessment. - Abstract: The integrated safety assessment (ISA) methodology, developed by the Spanish Nuclear Safety Council (CSN), has been applied to a thermal–hydraulic analysis of cold leg LOCA sequences with unavailable High Pressure Injection System in a Westinghouse 3-loop PWR. This analysis has been performed with TRACE 5.0 patch 1 code. ISA methodology allows obtaining the Damage Domain (the region of space of parameters where a safety limit is exceeded) as a function of uncertain parameters (break area) and operator actuation times, and provides to the analyst useful information about the impact of these uncertain parameters in safety concerns. In this work two main issues have been analyzed: the effect of reactor coolant pump trip and the available time for beginning of secondary-side depressurization. The main conclusions are that present Emergency Operating Procedures (EOPs) are adequate for managing this kind of sequences and the ISA methodology is able to take into account time delays and parameter uncertainties

Developing educational hypermedia applications: a methodological approach

Directory of Open Access Journals (Sweden)

Jose Miguel Nunes

1996-01-01

Full Text Available This paper proposes an hypermedia development methodology with the aim of integrating the work of both educators, who will be primarily responsible for the instructional design, with that of software experts, responsible for the software design and development. Hence, it is proposed that the educators and programmers should interact in an integrated and systematic manner following a methodological approach.

Research note: the performance of spring- and summer-reared broilers as affected by precision beak trimming at seven days of age.

Science.gov (United States)

Christmas, R B

1993-12-01

In each of two duplicate trials approximately 2,500 day-old Peterson x Arbor Acres straight-run broiler chicks were equally divided between two treatments of three pens each. Treatment 1 was maintained as controls (C), and Treatment 2 birds were precision beak-trimmed (PBT) at 7 days of age. Feed and water were supplied for ad libitum consumption to both groups. Trials 1 and 2 were initiated in March and June, respectively. Performance of spring-reared broilers were comparable regardless of beak trimming procedure, except that PBT broilers experienced slightly higher mortality after PBT. Final body weights of the summer-reared broilers were 15% lower than those reared in the spring. Additionally, PBT resulted in significantly reduced final body weights and feed intake. There were no significant differences in mortality or feed conversion due to PBT.

A single amino acid of human immunodeficiency virus type 2 capsid protein affects conformation of two external loops and viral sensitivity to TRIM5α.

Directory of Open Access Journals (Sweden)

Tadashi Miyamoto

Full Text Available We previously reported that human immunodeficiency virus type 2 (HIV-2 carrying alanine or glutamine but not proline at position 120 of the capsid protein (CA could grow in the presence of anti-viral factor TRIM5α of cynomolgus monkey (CM. To elucidate details of the interaction between the CA and TRIM5α, we generated mutant HIV-2 viruses, each carrying one of the remaining 17 possible amino acid residues, and examined their sensitivity to CM TRIM5α-mediated restriction. Results showed that hydrophobic residues or those with ring structures were associated with sensitivity, while those with small side chains or amide groups conferred resistance. Molecular dynamics simulation study revealed a structural basis for the differential TRIM5α sensitivities. The mutations at position 120 in the loop between helices 6 and 7 (L6/7 affected conformation of the neighboring loop between helices 4 and 5 (L4/5, and sensitive viruses had a common L4/5 conformation. In addition, the common L4/5 structures of the sensitive viruses were associated with a decreased probability of hydrogen bond formation between the 97th aspartic acid in L4/5 and the 119th arginine in L6/7. When we introduced aspartic acid-to-alanine substitution at position 97 (D97A of the resistant virus carrying glutamine at position 120 to disrupt hydrogen bond formation, the resultant virus became moderately sensitive. Interestingly, the virus carrying glutamic acid at position 120 showed resistance, while its predicted L4/5 conformation was similar to those of sensitive viruses. The D97A substitution failed to alter the resistance of this particular virus, indicating that the 120th amino acid residue itself is also involved in sensitivity regardless of the L4/5 conformation. These results suggested that a hydrogen bond between the L4/5 and L6/7 modulates the overall structure of the exposed surface of the CA, but the amino acid residue at position 120 is also directly involved in CM TRIM5�

Toll-like receptor 3 stimulation promotes Ro52/TRIM21 synthesis and nuclear redistribution in salivary gland epithelial cells, partially via type I interferon pathway

Science.gov (United States)

Kyriakidis, N C; Kapsogeorgou, E K; Gourzi, V C; Konsta, O D; Baltatzis, G E; Tzioufas, A G

2014-01-01

Up-regulated expression of Ro52/tripartite motif-containing protein 21 (TRIM21), Ro60/TROVE domain family, member 2 (TROVE2) and lupus LA protein/Sjögren's syndrome antigen B (La/SSB) autoantigens has been described in the salivary gland epithelial cells (SGEC) of patients with Sjögren's syndrome (SS). SGECs, the key regulators of autoimmune SS responses, express high levels of surface functional Toll-like receptor (TLR)-3, whereas Ro52/TRIM21 negatively regulates TLR-3-mediated inflammation. Herein, we investigated the effect of TLR-3-signalling on the expression of Ro52/TRIM21, as well as Ro60/TROVE2 and La/SSB autoantigens, by SGECs. The effect of TLR-3 or TLR-4 stimulation on autoantigen expression was evaluated by polyI:C or lipopolysaccharide (LPS) treatment, respectively, of SGEC lines (10 from SS patients, 12 from non-SS controls) or HeLa cells, followed by analysis of mRNA and protein expression. PolyI:C, but not LPS, resulted in a two-step induction of Ro52/TRIM21 mRNA expression by SGECs, a 12-fold increment at 6 h followed by a 2·5-fold increment at 24–48 h, whereas it induced a late two-fold up-regulation of Ro60/TROVE2 and La/SSB mRNAs at 48 h. Although protein expression levels were not affected significantly, the late up-regulation of Ro52/TRIM21 mRNA was accompanied by protein redistribution, from nucleolar-like pattern to multiple coarse dots spanning throughout the nucleus. These late phenomena were mediated significantly by interferon (IFN)-β production, as attested by cognate secretion and specific inhibition experiments and associated with IFN regulatory factor (IRF)3 degradation. TLR-3-signalling had similar effects on SGECs obtained from SS patients and controls, whereas it did not affect the expression of these autoantigens in HeLa cells. TLR-3 signalling regulates the expression of autoantigens by SGECs, implicating innate immunity pathways in their over-expression in inflamed tissues and possibly in their exposure to the immune

Preliminary safety analysis methodology for the SMART

Energy Technology Data Exchange (ETDEWEB)

Bae, Kyoo Hwan; Chung, Y. J.; Kim, H. C.; Sim, S. K.; Lee, W. J.; Chung, B. D.; Song, J. H. [Korea Atomic Energy Research Institute, Taejeon (Korea)

2000-03-01

This technical report was prepared for a preliminary safety analysis methodology of the 330MWt SMART (System-integrated Modular Advanced ReacTor) which has been developed by Korea Atomic Energy Research Institute (KAERI) and funded by the Ministry of Science and Technology (MOST) since July 1996. This preliminary safety analysis methodology has been used to identify an envelope for the safety of the SMART conceptual design. As the SMART design evolves, further validated final safety analysis methodology will be developed. Current licensing safety analysis methodology of the Westinghouse and KSNPP PWRs operating and under development in Korea as well as the Russian licensing safety analysis methodology for the integral reactors have been reviewed and compared to develop the preliminary SMART safety analysis methodology. SMART design characteristics and safety systems have been reviewed against licensing practices of the PWRs operating or KNGR (Korean Next Generation Reactor) under construction in Korea. Detailed safety analysis methodology has been developed for the potential SMART limiting events of main steam line break, main feedwater pipe break, loss of reactor coolant flow, CEA withdrawal, primary to secondary pipe break and the small break loss of coolant accident. SMART preliminary safety analysis methodology will be further developed and validated in parallel with the safety analysis codes as the SMART design further evolves. Validated safety analysis methodology will be submitted to MOST as a Topical Report for a review of the SMART licensing safety analysis methodology. Thus, it is recommended for the nuclear regulatory authority to establish regulatory guides and criteria for the integral reactor. 22 refs., 18 figs., 16 tabs. (Author)

An IMM-Aided ZUPT Methodology for an INS/DVL Integrated Navigation System.

Science.gov (United States)

Yao, Yiqing; Xu, Xiaosu; Xu, Xiang

2017-09-05

Inertial navigation system (INS)/Doppler velocity log (DVL) integration is the most common navigation solution for underwater vehicles. Due to the complex underwater environment, the velocity information provided by DVL always contains some errors. To improve navigation accuracy, zero velocity update (ZUPT) technology is considered, which is an effective algorithm for land vehicles to mitigate the navigation error during the pure INS mode. However, in contrast to ground vehicles, the ZUPT solution cannot be used directly for underwater vehicles because of the existence of the water current. In order to leverage the strengths of the ZUPT method and the INS/DVL solution, an interactive multiple model (IMM)-aided ZUPT methodology for the INS/DVL-integrated underwater navigation system is proposed. Both the INS/DVL and INS/ZUPT models are constructed and operated in parallel, with weights calculated according to their innovations and innovation covariance matrices. Simulations are conducted to evaluate the proposed algorithm. The results indicate that the IMM-aided ZUPT solution outperforms both the INS/DVL solution and the INS/ZUPT solution in the underwater environment, which can properly distinguish between the ZUPT and non-ZUPT conditions. In addition, during DVL outage, the effectiveness of the proposed algorithm is also verified.

An end-users oriented methodology for enhancing the integration of knowledge on soil-water-sediment systems in River Basin Management: an illustration from the AquaTerra project.

Science.gov (United States)

Merly, Corinne; Chapman, Antony; Mouvet, Christophe

2012-01-01

Research results in environmental and socio-economic sciences are often under-used by stakeholders involved in the management of natural resources. To minimise this gap, the FP6 EU interdisciplinary project AquaTerra (AT) developed an end-users' integration methodology in order to ensure that the data, knowledge and tools related to the soil-water-sediment system that were generated by the project were delivered in a meaningful way for end-users, thus improving their uptake. The methodology and examples of its application are presented in this paper. From the 408 project deliverables, 96 key findings were identified, 53 related to data and knowledge, and 43 describing advanced tools. River Basin Management (RBM) stakeholders workshops identified 8 main RBM issues and 25 specific stakeholders' questions related to RBM which were classified into seven groups of cross-cutting issues, namely scale, climate change, non-climatic change, the need for systemic approaches, communication and participation, international and inter-basin coordination and collaboration, and the implementation of the Water Framework Directive. The integration methodology enabled an assessment of how AT key findings meet stakeholders' demands, and for each main RBM issue and for each specific question, described the added-value of the AT project in terms of knowledge and tools generated, key parameters to consider, and recommendations that can be made to stakeholders and the wider scientific community. Added value and limitations of the integration methodology and its outcomes are discussed and recommendations are provided to further improve integration methodology and bridge the gaps between scientific research data and their potential uptake by end-users.

Tornado missile simulation and design methodology. Volume 1: simulation methodology, design applications, and TORMIS computer code. Final report

International Nuclear Information System (INIS)

Twisdale, L.A.; Dunn, W.L.

1981-08-01

A probabilistic methodology has been developed to predict the probabilities of tornado-propelled missiles impacting and damaging nuclear power plant structures. Mathematical models of each event in the tornado missile hazard have been developed and sequenced to form an integrated, time-history simulation methodology. The models are data based where feasible. The data include documented records of tornado occurrence, field observations of missile transport, results of wind tunnel experiments, and missile impact tests. Probabilistic Monte Carlo techniques are used to estimate the risk probabilities. The methodology has been encoded in the TORMIS computer code to facilitate numerical analysis and plant-specific tornado missile probability assessments. Sensitivity analyses have been performed on both the individual models and the integrated methodology, and risk has been assessed for a hypothetical nuclear power plant design case study

Methodological Pluralism and Narrative Inquiry

Science.gov (United States)

Michie, Michael

2013-01-01

This paper considers how the integral theory model of Nancy Davis and Laurie Callihan might be enacted using a different qualitative methodology, in this case the narrative methodology. The focus of narrative research is shown to be on "what meaning is being made" rather than "what is happening here" (quadrant 2 rather than…

Screening radon risks: A methodology for policymakers

International Nuclear Information System (INIS)

Eisinger, D.S.; Simmons, R.A.; Lammering, M.; Sotiros, R.

1991-01-01

This paper provides an easy-to-use screening methodology to estimate potential excess lifetime lung cancer risk resulting from indoor radon exposure. The methodology was developed under U.S. EPA Office of Policy, Planning, and Evaluation sponsorship of the agency's Integrated Environmental Management Projects (IEMP) and State/Regional Comparative Risk Projects. These projects help policymakers understand and use scientific data to develop environmental problem-solving strategies. This research presents the risk assessment methodology, discusses its basis, and identifies appropriate applications. The paper also identifies assumptions built into the methodology and qualitatively addresses methodological uncertainties, the direction in which these uncertainties could bias analyses, and their relative importance. The methodology draws from several sources, including risk assessment formulations developed by the U.S. EPA's Office of Radiation Programs, the EPA's Integrated Environmental Management Project (Denver), the International Commission on Radiological Protection, and the National Institute for Occupational Safety and Health. When constructed as a spreadsheet program, the methodology easily facilitates analyses and sensitivity studies (the paper includes several sensitivity study options). The methodology will be most helpful to those who need to make decisions concerning radon testing, public education, and exposure prevention and mitigation programs.26 references

Thrust Augmentation by Airframe-Integrated Linear-Spike Nozzle Concept for High-Speed Aircraft

Directory of Open Access Journals (Sweden)

Hidemi Takahashi

2018-02-01

Full Text Available The airframe-integrated linear-spike nozzle concept applied to an external nozzle for high-speed aircraft was evaluated with regard to the thrust augmentation capability and the trim balance. The main focus was on the vehicle aftbody. The baseline airframe geometry was first premised to be a hypersonic waverider design. The baseline aftbody case had an external nozzle comprised of a simple divergent nozzle and was hypothetically replaced with linear-spike external nozzle configurations. Performance evaluation was mainly conducted by considering the nozzle thrust generated by the pressure distribution on the external nozzle surface at the aftbody portion calculated by computer simulation at a given cruise condition with zero angle of attack. The thrust performance showed that the proposed linear-spike external nozzle concept was beneficial in thrust enhancement compared to the baseline geometry because the design of the proposed concept had a compression wall for the exhaust flow, which resulted in increasing the wall pressure. The configuration with the boattail and the angled inner nozzle exhibited further improvement in thrust performance. The trim balance evaluation showed that the aerodynamic center location appeared as acceptable. Thus, benefits were obtained by employing the airframe-integrated linear-spike external nozzle concept.

Reliability Centered Maintenance - Methodologies

Science.gov (United States)

Kammerer, Catherine C.

2009-01-01

Journal article about Reliability Centered Maintenance (RCM) methodologies used by United Space Alliance, LLC (USA) in support of the Space Shuttle Program at Kennedy Space Center. The USA Reliability Centered Maintenance program differs from traditional RCM programs because various methodologies are utilized to take advantage of their respective strengths for each application. Based on operational experience, USA has customized the traditional RCM methodology into a streamlined lean logic path and has implemented the use of statistical tools to drive the process. USA RCM has integrated many of the L6S tools into both RCM methodologies. The tools utilized in the Measure, Analyze, and Improve phases of a Lean Six Sigma project lend themselves to application in the RCM process. All USA RCM methodologies meet the requirements defined in SAE JA 1011, Evaluation Criteria for Reliability-Centered Maintenance (RCM) Processes. The proposed article explores these methodologies.

77 FR 58091 - Risk-Based Sampling of Beef Manufacturing Trimmings for Escherichia coli (E. coli) O157:H7 and...

Science.gov (United States)

2012-09-19

... for exposure of carcasses and parts to any contamination or food safety hazard during the removal of... DEPARTMENT OF AGRICULTURE Food Safety and Inspection Service [Docket No. FSIS-2012-0020] Risk-Based Sampling of Beef Manufacturing Trimmings for Escherichia coli (E. coli) O157:H7 and Plans for Beef...

Development Methodology for an Integrated Legal Cadastre

NARCIS (Netherlands)

Hespanha, J.P.

2012-01-01

This Thesis describes the research process followed in order to achieve a development methodology applicable to the reform of cadastral systems with a legal basis. It was motivated by the author’s participation in one of the first surveying and mapping operations for a digital cadastre in Portugal,

Soda-anthraquinone, kraft and organosolv pulping of holm oak trimmings.

Science.gov (United States)

Alaejos, J; López, F; Eugenio, M E; Tapias, R

2006-11-01

The operating conditions for an organosolv (ethyleneglycol) and two alkaline (soda-anthraquinone and kraft) processes for obtaining cellulose pulp and paper from holm oak (Quercus ilex) wood trimmings were optimized. A range of variation for each process variable (viz. temperature, cooking time and soda or ethyleneglycol concentration) was established and a central composite experimental design involving three independent variables at three different variation levels was applied. The results obtained with the three cooking processes used were compared and those provided by the kraft process were found to be the best. Thus, the tensile index values it provided (5.9-16.3 N m/g) were 23.7% and 41.5% better than those obtained with the soda-AQ and ethyleneglycol processes, respectively. Also, the kraft process provided the best burst index, brightness and kappa number values. Based on the optimum working ranges, the temperature and cooking time were the variables resulting in the most and least marked changes, respectively, in pulp properties.

A Methodology for Multiple Rule System Integration and Resolution Within a Singular Knowledge Base

Science.gov (United States)

Kautzmann, Frank N., III

1988-01-01

Expert Systems which support knowledge representation by qualitative modeling techniques experience problems, when called upon to support integrated views embodying description and explanation, especially when other factors such as multiple causality, competing rule model resolution, and multiple uses of knowledge representation are included. A series of prototypes are being developed to demonstrate the feasibility of automating the process of systems engineering, design and configuration, and diagnosis and fault management. A study involves not only a generic knowledge representation; it must also support multiple views at varying levels of description and interaction between physical elements, systems, and subsystems. Moreover, it will involve models of description and explanation for each level. This multiple model feature requires the development of control methods between rule systems and heuristics on a meta-level for each expert system involved in an integrated and larger class of expert system. The broadest possible category of interacting expert systems is described along with a general methodology for the knowledge representation and control of mutually exclusive rule systems.

An integrated probabilistic risk analysis decision support methodology for systems with multiple state variables

International Nuclear Information System (INIS)

Sen, P.; Tan, John K.G.; Spencer, David

1999-01-01

Probabilistic risk analysis (PRA) methods have been proven to be valuable in risk and reliability analysis. However, a weak link seems to exist between methods for analysing risks and those for making rational decisions. The integrated decision support system (IDSS) methodology presented in this paper attempts to address this issue in a practical manner. In consists of three phases: a PRA phase, a risk sensitivity analysis (SA) phase and an optimisation phase, which are implemented through an integrated computer software system. In the risk analysis phase the problem is analysed by the Boolean representation method (BRM), a PRA method that can deal with systems with multiple state variables and feedback loops. In the second phase the results obtained from the BRM are utilised directly to perform importance and risk SA. In the third phase, the problem is formulated as a multiple objective decision making problem in the form of multiple objective reliability optimisation. An industrial example is included. The resultant solutions of a five objective reliability optimisation are presented, on the basis of which rational decision making can be explored

An integrative approach to the design methodology for 3-phase power conditioners in Photovoltaic Grid-Connected systems

International Nuclear Information System (INIS)

Rey-Boué, Alexis B.; García-Valverde, Rafael; Ruz-Vila, Francisco de A.; Torrelo-Ponce, José M.

2012-01-01

Highlights: ► A design methodology for Photovoltaic grid-connected systems is presented. ► Models of the Photovoltaic Generator and the 3-phase Inverter are described. ► The power factor and the power quality are regulated with vector control. ► Simulation and experimental results validate the design methodology. ► The proposed methodology can be extended to any Renewable or Industrial System. - Abstract: A novel methodology is presented in this paper, for the design of the Power and Control Subsystems of a 3-phase Photovoltaic Grid-Connected system in an easy and comprehensive way, as an integrative approach. At the DC side of the Power Subsystem, the Photovoltaic Generator modeling is revised and a simple model is proposed, whereas at the AC side, a vector analysis is done to deal with the instantaneous 3-phase variables of the grid-connected Voltage Source Inverter. A d–q control approach is established in the Control Subsystem, along with its specific tuned parameters, as a vector control alternative which will allow the decoupled control of the instantaneous active and reactive powers. A particular Case of Study is presented to illustrate the behavior of the design methodology regarding the fulfillment of the Photovoltaic plant specifications. Some simulations are run to study the performance of the Photovoltaic Generator together with the exerted d–q control to the grid-connected 3-phase inverter, and some experimental results, obtained from a built flexible platform, are also shown. The simulations and the experimental results validate the overall performance of the 3-phase Photovoltaic Grid-Connected system due to the attained unitary power factor operation together with good power quality. The final validation of the proposed design methodology is also achieved.

Methodology of Adaptive Integrated Accounting System in Information Environment

Directory of Open Access Journals (Sweden)

Bochulya Tetyana V.

2013-12-01

Full Text Available The goal of the article lies in the study of logical and methodological justification of formation of the integrated system of accounting based on realities of the co-ordinated transformation of the society and economy and development of a new knowledge about formation and adjustment of the accounting system in it’s a priori new information competence with expansion of functionality for the justified idea of existence and development of business. Taking developments of the best representatives of the leading scientific society as a basis, the article offers a new vision of organisation of the accounting system, based on the modern projection of information competence and harmonisation of main processes of information service for adaptation of the system for multi-vector inquiries of consumers of information. Pursuant to results of the conducted study, the article makes an effort to change the established opinion about information and professional competences of the accounting system and attach a new qualitative significance to them. The article makes a proposal with respect to calculation of quality of the information system on the basis of key indicators of its information service. It lays the foundation of the prospective study of the problems of building the accounting system in such a projection, so that realities of internal and external processes were maximally co-ordinated based on the idea of their information development.

Comparative economic analysis of the Integral Molten Salt Reactor and an advanced PWR using the G4-ECONS methodology

International Nuclear Information System (INIS)

Samalova, Ludmila; Chvala, Ondrej; Maldonado, G. Ivan

2017-01-01

The assessment of economic viability of a new reactor concept is crucial particularly during the early stages of its concept development. The G4-ECONS methodology provides a standardized top-down estimate of electricity cost and parametric sensitivities, not specifically targeted toward an accurate prediction of the final cost when deployed, but rather seeking an approximation of cost variations relative to other systems. This study presents an analysis of the Integral Molten Salt Reactor (IMSR) concept in comparison with a consistent analysis of an advanced PWR reactor (represented by AP1000). Estimation of levelized unit electricity costs, as well as sensitivity analyses to the discount rate and uranium or SWU prices, are presented using this methodology.

Novel trimming technique for tunable HTS microstrip filters

Energy Technology Data Exchange (ETDEWEB)

Sekiya, N. [Department of Electrical Engineering, Yamanashi University, Nakagawa-Sekiya Laboratory, 4-3-11 Takeda, Kofu 400-8511 (Japan)], E-mail: nsekiya@yamanashi.ac.jp; Nakagawa, Y. [Department of Electrical Engineering, Yamanashi University, Nakagawa-Sekiya Laboratory, 4-3-11 Takeda, Kofu 400-8511 (Japan); Saito, A.; Ohshima, S. [Yamagata University, 4-3-16 Johnan, Yonezawa 992-8510 (Japan)

2008-09-15

We have developed a method using additional electric pads for trimming tunable high-temperature superconducting (HTS) microstrip filters. These filters are generally tuned by adjusting the gap between a dielectric floating plate above the filter. When the floating plate approached the filter, the center frequency was shifted to a lower frequency. However, the insertion loss increases due to variation in the external quality factors varying from the design parameter. The external quality factors are usually controlled by adjusting the length of the input/output (I/O) coupled-line elements and the gap between the elements and the resonator. In our method, additional electric pads are distributed at the open-end of the I/O coupled-line elements of a 3-pole hairpin bandpass filter to enable adjustment of the external quality factors so as to reduce insertion loss. The electric pads consist of line-and-space patterns. They are eclectically connected to the coupled-line elements to adjust the line length and gap width and thereby control the external quality factors. An electromagnetic simulator was used for the design and analysis. The simulation results showed that the additional electric pads are effective in improving the insertion loss of the HTS bandpass filter after tuning.

Novel trimming technique for tunable HTS microstrip filters

International Nuclear Information System (INIS)

Sekiya, N.; Nakagawa, Y.; Saito, A.; Ohshima, S.

2008-01-01

We have developed a method using additional electric pads for trimming tunable high-temperature superconducting (HTS) microstrip filters. These filters are generally tuned by adjusting the gap between a dielectric floating plate above the filter. When the floating plate approached the filter, the center frequency was shifted to a lower frequency. However, the insertion loss increases due to variation in the external quality factors varying from the design parameter. The external quality factors are usually controlled by adjusting the length of the input/output (I/O) coupled-line elements and the gap between the elements and the resonator. In our method, additional electric pads are distributed at the open-end of the I/O coupled-line elements of a 3-pole hairpin bandpass filter to enable adjustment of the external quality factors so as to reduce insertion loss. The electric pads consist of line-and-space patterns. They are eclectically connected to the coupled-line elements to adjust the line length and gap width and thereby control the external quality factors. An electromagnetic simulator was used for the design and analysis. The simulation results showed that the additional electric pads are effective in improving the insertion loss of the HTS bandpass filter after tuning

Transcriptional activator TAp63 is upregulated in muscular atrophy during ALS and induces the pro-atrophic ubiquitin ligase Trim63

Science.gov (United States)

von Grabowiecki, Yannick; Abreu, Paula; Blanchard, Orphee; Palamiuc, Lavinia; Benosman, Samir; Mériaux, Sophie; Devignot, Véronique; Gross, Isabelle; Mellitzer, Georg; Gonzalez de Aguilar, José L; Gaiddon, Christian

2016-01-01

Mechanisms of muscle atrophy are complex and their understanding might help finding therapeutic solutions for pathologies such as amyotrophic lateral sclerosis (ALS). We meta-analyzed transcriptomic experiments of muscles of ALS patients and mouse models, uncovering a p53 deregulation as common denominator. We then characterized the induction of several p53 family members (p53, p63, p73) and a correlation between the levels of p53 family target genes and the severity of muscle atrophy in ALS patients and mice. In particular, we observed increased p63 protein levels in the fibers of atrophic muscles via denervation-dependent and -independent mechanisms. At a functional level, we demonstrated that TAp63 and p53 transactivate the promoter and increased the expression of Trim63 (MuRF1), an effector of muscle atrophy. Altogether, these results suggest a novel function for p63 as a contributor to muscular atrophic processes via the regulation of multiple genes, including the muscle atrophy gene Trim63. DOI: http://dx.doi.org/10.7554/eLife.10528.001 PMID:26919175

Considering axiological integrity: a methodological analysis of qualitative evidence syntheses, and its implications for health professions education.

Science.gov (United States)

Kelly, Martina; Ellaway, Rachel H; Reid, Helen; Ganshorn, Heather; Yardley, Sarah; Bennett, Deirdre; Dornan, Tim

2018-05-14

Qualitative evidence synthesis (QES) is a suite of methodologies that combine qualitative techniques with the synthesis of qualitative knowledge. They are particularly suited to medical education as these approaches pool findings from original qualitative studies, whilst paying attention to context and theoretical development. Although increasingly sophisticated use is being made of qualitative primary research methodologies in health professions education (HPE) the use of secondary qualitative reviews in HPE remains underdeveloped. This study examined QES methods applied to clinical humanism in healthcare as a way of advancing thinking around the use of QES in HPE in general. A systematic search strategy identified 49 reviews that fulfilled the inclusion criteria. Meta-study was used to develop an analytic summary of methodological characteristics, the role of theory, and the synthetic processes used in QES reviews. Fifteen reviews used a defined methodology, and 17 clearly explained the processes that led from data extraction to synthesis. Eight reviews adopted a specific theoretical perspective. Authors rarely described their reflexive relationship with their data. Epistemological positions tended to be implied rather than explicit. Twenty-five reviews included some form of quality appraisal, although it was often unclear how authors acted on its results. Reviewers under-reported qualitative approaches in their review methodologies, and tended to focus on elements such as systematicity and checklist quality appraisal that were more germane to quantitative evidence synthesis. A core concern was that the axiological (value) dimensions of the source materials were rarely considered let alone accommodated in the synthesis techniques used. QES can be used in HPE research but only with careful attention to maintaining axiological integrity.

An integrated quality function deployment and capital budgeting methodology for occupational safety and health as a systems thinking approach: the case of the construction industry.

Science.gov (United States)

Bas, Esra

2014-07-01

In this paper, an integrated methodology for Quality Function Deployment (QFD) and a 0-1 knapsack model is proposed for occupational safety and health as a systems thinking approach. The House of Quality (HoQ) in QFD methodology is a systematic tool to consider the inter-relationships between two factors. In this paper, three HoQs are used to consider the interrelationships between tasks and hazards, hazards and events, and events and preventive/protective measures. The final priority weights of events are defined by considering their project-specific preliminary weights, probability of occurrence, and effects on the victim and the company. The priority weights of the preventive/protective measures obtained in the last HoQ are fed into a 0-1 knapsack model for the investment decision. Then, the selected preventive/protective measures can be adapted to the task design. The proposed step-by-step methodology can be applied to any stage of a project to design the workplace for occupational safety and health, and continuous improvement for safety is endorsed by the closed loop characteristic of the integrated methodology. Copyright © 2013 Elsevier Ltd. All rights reserved.

Longitudinal Trim and Tumble Characteristics of a 0.057-Scale Model of the Chance Vought XF7U-1 Airplane, TED NO. NACA DE311

Science.gov (United States)

Bryant, Robert L.

1948-01-01

Based on results of longitudinal trim and tumble tests of a 0.057-scale model of the Chance Vought XF7U-1 airplane, the following conclusions regarding the trim and tumble characteristics of the airplane have been drawn: 1. The airplane will not trim at any unusual or uncontrolled angles of attack. 2. The airplane will not tumble with the center of gravity located forward of 24 percent of the mean aerodynamic chord. When the center of gravity is located at 24 percent of the mean aerodynamic chord and slats are extended and elevators are deflected full up, the airplane may tumble if given an external positive pitching moment. 3. The tumbling motion obtained will be readily terminated by deflecting the elevators full down so as to oppose the rotation. 4. The accelerations encountered during an established tumble may be dangerous to the pilot and, therefore, action should be taken to terminate a tumble immediately upon its inception. 5. Simultaneous opening of two wing-tip parachutes having diameters of 4 feet or larger and having drag coefficients of approximately 0.7 will effectively terminate the tumble. 6. Model results indicate that the pilot will not be struck by the airplane if it becomes necessary to leave the airplane during a tumble. The pilot may require aid from an ejection-seat arrangement.

Integrated management of operations in Santos Basin: methodology applied to a new philosophy of operations

Energy Technology Data Exchange (ETDEWEB)

Pereira, Leandro Leonardo; Lima, Claudio Benevenuto de Campos [Petroleo Brasileiro S.A. (PETROBRAS), Rio de Janeiro, RJ (Brazil); Derenzi Neto, Dario [Accenture, Rio de Janeiro, RJ (Brazil); Pinto, Vladimir Steffen [Soda IT, Rio de Janeiro, RJ (Brazil); Lima, Gilson Brito Alves [Universidade Federal Fluminense (UFF), Niteroi, RJ (Brazil)

2012-07-01

The objective of this paper is to present the methodology used to develop the Integrated Management of Operations (GIOp) project in Santos Basin Operational Unit (UO-BS) in the South-Southeast Exploration and Production area of PETROBRAS. The following text describes how the activities were carried out to gather improvements opportunities and to design To-Be processes, considering the challenging environment of the Santos Basin in the coming years. At the end of more than 12 months of work, more than 50 processes and sub-processes were redesigned, involving a multidisciplinary team in the areas of operations, maintenance, safety, health and environment, flow assurance, wells, reservoirs and planning. (author)

Methodology for Supply Chain Integration: A Case Study in the Artisan Industry of Footwear

Directory of Open Access Journals (Sweden)

Jorge Raúl Pérez-Gallardo

2014-01-01

Full Text Available The supply chain is a major issue in a global market. The efficient relationship between supplier-producer-retailer conforms the perfect combination to achieve a high level of competitiveness according to the needs of increasingly demanding and changing markets. The difficulty of synchronizing the members within the SC is related to the inherent uncertain factors. This paper proposes a methodology for integrating the supply chain that can be applied in any type of business. To demonstrate its relevance, a case study is performed at a handmade shoe company. An improved demand forecasting, an optimal balanced production line, a proper inventory management of raw materials, and an efficient calculation of the delivery time to the customer represent some of the main results.

Integrated analytical methodologies for the study of corrosion processes in archaeological bronzes

Energy Technology Data Exchange (ETDEWEB)

Alberghina, Maria Francesca; Barraco, Rosita; Brai, Maria; Schillaci, Tiziano, E-mail: tschillaci@unipa.it; Tranchina, Luigi

2011-02-15

The investigations on structure and micro-chemical composition of archaeological metal alloys are needed in archaeometry. The aim of this study is devoted both to acquire information about their provenance and production technology, and to improve our understanding about the corrosion processes. In this paper we present the study of the corrosion phenomena of bronze samples, laboratory-made according to binary, ternary and quaternary alloys typical of Roman archaeometallurgical production through an integrated methodology based on the use of non or micro invasive physical techniques. Among the analysed samples, two were artificially aged through burial in the archaeological site of Tharros, along the west coast of Sardinia (Italy). The corrosion products, typical of the bronzes in archaeological sites near the sea, have been characterized by non invasive and micro-destructive measurements. In particular, the corrosion patinas were examined through optical microscopy, scanning electron microscopy and microanalysis, X-ray fluorescence and laser ablation spectroscopy. The use of integrated technologies allowed us to determine both the elemental composition and surface morphology of the patina, highlighting the correlation between patina nature and chemical composition of the burial context. Moreover, data obtained by the laser-induced breakdown spectroscopy along the depth profile on the samples, have yielded information about the stratigraphic layers of corrosion products and their growth. Finally, the depth profiles allowed us to verify both the chemical elements constituting the patina, the metal ions constituting the alloy and the occurrence of migration phenomena from bulk to the surface.

Integrated analytical methodologies for the study of corrosion processes in archaeological bronzes

International Nuclear Information System (INIS)

Alberghina, Maria Francesca; Barraco, Rosita; Brai, Maria; Schillaci, Tiziano; Tranchina, Luigi

2011-01-01

The investigations on structure and micro-chemical composition of archaeological metal alloys are needed in archaeometry. The aim of this study is devoted both to acquire information about their provenance and production technology, and to improve our understanding about the corrosion processes. In this paper we present the study of the corrosion phenomena of bronze samples, laboratory-made according to binary, ternary and quaternary alloys typical of Roman archaeometallurgical production through an integrated methodology based on the use of non or micro invasive physical techniques. Among the analysed samples, two were artificially aged through burial in the archaeological site of Tharros, along the west coast of Sardinia (Italy). The corrosion products, typical of the bronzes in archaeological sites near the sea, have been characterized by non invasive and micro-destructive measurements. In particular, the corrosion patinas were examined through optical microscopy, scanning electron microscopy and microanalysis, X-ray fluorescence and laser ablation spectroscopy. The use of integrated technologies allowed us to determine both the elemental composition and surface morphology of the patina, highlighting the correlation between patina nature and chemical composition of the burial context. Moreover, data obtained by the laser-induced breakdown spectroscopy along the depth profile on the samples, have yielded information about the stratigraphic layers of corrosion products and their growth. Finally, the depth profiles allowed us to verify both the chemical elements constituting the patina, the metal ions constituting the alloy and the occurrence of migration phenomena from bulk to the surface.

Snavelbehandeling in de kalkoenhouderij : alternatieven via fokkerij en bedrijfsmanagemrnt = Beak trimming in turkey production : alternatives by means of breeding and farm management

NARCIS (Netherlands)

Veldkamp, T.

2010-01-01

A literature review on effects of breeding and management on injurious pecking behaviour and cannibalism has been conducted. The combination of factors to avoid injurious behaviour is not clear and thus no good alternative to beak trimming of the upper beak in day-old turkey poults is available. If

Proposition of a modeling and an analysis methodology of integrated reverse logistics chain in the direct chain

Energy Technology Data Exchange (ETDEWEB)

Mimouni, F.; Abouabdellah, A.

2016-07-01

Propose a modeling and analysis methodology based on the combination of Bayesian networks and Petri networks of the reverse logistics integrated the direct supply chain. Network modeling by combining Petri and Bayesian network. Modeling with Bayesian network complimented with Petri network to break the cycle problem in the Bayesian network. Demands are independent from returns. Model can only be used on nonperishable products. Legislation aspects: Recycling laws; Protection of environment; Client satisfaction via after sale service. Bayesian network with a cycle combined with the Petri Network. (Author)

Modeling of trim panels in the energy finite element analysis

Science.gov (United States)

Moravaeji, Seyed-Javid

Modeling a trim panel is divided into finding the power exchange through two different paths: (i) the connection of the outer and inner panels (ii) through the layers directly. The vibrational power exchanged through the mounts is modeled as the connection of two parallel plates connected via a beam. Wave matrices representing plates and beams are derived separately; then a matrix method is proposed to solve for the wave amplitudes and hence the vibrational power exchange between the plates accordingly. A closed form formula for the case of connection of two identical plates is derived. For the power transmission loss directly through the layers, first transfer matrices representing layers made of different materials is considered. New matrices for a porous layer are derived. A method of finding the layered structure transfer matrix is proposed. It is concluded that in general a single isotropic layer cannot replace a structure accurately. Finally, on the basis of an equivalent transfer matrix, an optimization process for is proposed to replace the panel by a suitable set of layers.

Integration of a solar thermal system in a medium-sized brewery using pinch analysis: Methodology and case study

International Nuclear Information System (INIS)

Eiholzer, Tobias; Olsen, Donald; Hoffmann, Sebastian; Sturm, Barbara; Wellig, Beat

2017-01-01

Highlights: • Methodologies to reduce energy consumption in batch processes are presented. • Pinch analysis is used to improve energy efficiency. • Integration potential for solar heat is presented on a Scottish brewery case study. • Governmental support is important in a company’s investment in renewables. - Abstract: In the food industry a major portion of thermal energy is required for low temperature applications (below 100 °C). As a consequence, there is a significant potential to substitute fossil fuels by the use of solar heat. This paper presents a methodology that first uses pinch analysis to optimize a medium-sized Scottish brewery from a direct heat recovery perspective followed by the integration of a solar thermal system. Both the time average model and time slice model were used to determine direct and indirect heat recovery potentials. In a second stage, an optimization of a chosen integration point was conducted to assess the viability of the resulting design concept. The economic analysis includes an assessment of the impact of restrictions in the UK government’s Renewable Heat Incentive program. It was determined that since solar thermal systems are financially supported up to an installed capacity of 200 kW, solar heat can only account for a maximum of 7.7% of the heat demand based on the investigated brewery. However, if there was no limitation in capacity, from an economic point of view, the solar fraction could almost be doubled drawing into question the need for the restriction. Nevertheless, a CO_2 saving potential of approximately 38 tons per year in conjunction with a payback period of 6.4 years was determined.

CAD-Based Modeling of Advanced Rotary Wing Structures for Integrated 3-D Aeromechanics Analysis

Science.gov (United States)

Staruk, William

This dissertation describes the first comprehensive use of integrated 3-D aeromechanics modeling, defined as the coupling of 3-D solid finite element method (FEM) structural dynamics with 3-D computational fluid dynamics (CFD), for the analysis of a real helicopter rotor. The development of this new methodology (a departure from how rotor aeroelastic analysis has been performed for 40 years), its execution on a real rotor, and the fundamental understanding of aeromechanics gained from it, are the key contributions of this dissertation. This work also presents the first CFD/CSD analysis of a tiltrotor in edgewise flight, revealing many of its unique loading mechanisms. The use of 3-D FEM, integrated with a trim solver and aerodynamics modeling, has the potential to enhance the design of advanced rotors by overcoming fundamental limitations of current generation beam-based analysis tools and offering integrated internal dynamic stress and strain predictions for design. Two primary goals drove this research effort: 1) developing a methodology to create 3-D CAD-based brick finite element models of rotors including multibody joints, controls, and aerodynamic interfaces, and 2) refining X3D, the US Army's next generation rotor structural dynamics solver featuring 3-D FEM within a multibody formulation with integrated aerodynamics, to model a tiltrotor in the edgewise conversion flight regime, which drives critical proprotor structural loads. Prior tiltrotor analysis has primarily focused on hover aerodynamics with rigid blades or forward flight whirl-flutter stability with simplified aerodynamics. The first goal was met with the development of a detailed methodology for generating multibody 3-D structural models, starting from CAD geometry, continuing to higher-order hexahedral finite element meshing, to final assembly of the multibody model by creating joints, assigning material properties, and defining the aerodynamic interface. Several levels of verification and

METHODOLOGY OF ORGANIZATION OF INTEGRATED LESSONS OF NATURAL-SCIENCE CYCLE (ON THE EXAMPLE OF TEACHING SPE STUDENTS

Directory of Open Access Journals (Sweden)

Alsou Raufovna Kamaleeva

2015-09-01

Full Text Available In the process of transition of Russian organizations of secondary professional education to educational standards of the third generation educational process is reduced to formation of students’ competences. This article presents methodology of creating integrated lessons of natural-science cycle (for example, in physics and informatics. These lessons are constructed on the basis of interdisciplinary integration and focused on task solution. The main purpose is to teach students how to solve particular tasks in physics with the use of informatics, in particular on the basis of algorithmization and programming (Pascal language. Didactic conditions, which are the basis of the algorithm of designing corresponding tasks, are described in this article. Structural components of the integrated lessons created on the traditional principle are marked out. During the research we observed that realization of all stages of the corresponding lessons in practice allows the teacher to create educational process over the borders of disciplinary basis. This approach helps to form generalization of knowledge. Being one of the most optimal forms of education, an integrated lesson allows students to solve various educational and professional problems in non-standard situations and stimulates their cognitive activity and their involvement in the process of education and their responsibility for the result which promotes an intensification of educational process.

Development of a standard methodology for integrating non-food crop production in rural areas with niche energy markets. Proceedings

International Nuclear Information System (INIS)

1996-09-01

This project was supported as a Concerted Action under the EC DGVI AIR programme from 1993-1996. It has successfully developed a standard methodology to help integrate non-food crop production in rural areas with niche energy markets. The methodology was used to compare the costs of different energy crop production and conversion options across the six participating nations. The partners provide a representative cross-section of European agriculture and energy expertise. All partners agreed on three niche markets favourable for biomass and biofuels: small-scale heat markets (less than 1 MW th ) for agro-industry, domestic and commercial buildings, medium-scale heat markets (1-10MW th ), including cogeneration for light industry and district heating, and liquid biofuels as substitutes for fossil fuels in transport, heat and power applications. (Author)

A methodology for development of biocatalytic processes

DEFF Research Database (Denmark)

Lima Ramos, Joana

are available. The first case study presents a rational approach for defining a development strategy for multi-enzymatic processes. The proposed methodology requires a profound and structured knowledge of the multi-enzyme systems, integrating chemistry, biological and process engineering. In order to suggest......). These process metrics can often be attained by improvements in the reaction chemistry, the biocatalyst, and/or by process engineering, which often requires a complex process development strategy. Interestingly this complexity, which arises from the need for integration of biological and process technologies...... and their relationship with the overall process is not clear.The work described in this thesis presents a methodological approach for early stage development of biocatalytic processes, understanding and dealing with the reaction, biocatalyst and process constraints. When applied, this methodology has a decisive role...

Imaginative methodologies in the social sciences

DEFF Research Database (Denmark)

Imaginative Methodologies develops, expands and challenges conventional social scientific methodology and language by way of literary, poetic and other alternative sources of inspiration. Sociologists, social workers, anthropologists, criminologists and psychologists all try to rethink, provoke...... and reignite social scientific methodology. Imaginative Methodologies challenges the mainstream social science methodological orthodoxy closely guarding the boundaries between the social sciences and the arts and humanities, pointing out that authors and artists are often engaged in projects parallel to those...... of the social sciences and vice versa, and that artistic and cultural productions today do not constitute a specialist field, but are integral to our social reality. The book will be of interest to scholars and students in the social sciences and across the arts and humanities working with questions...

What the Current System Development Trends tell us about Systems Development Methodologies: Toward explaining SSDAM, Agile and IDEF0 Methodologies

Directory of Open Access Journals (Sweden)

Abdulla F. Ally

2015-03-01

Full Text Available Systems integration, customization and component based development approach are of increasing attention. This trend facilitates the research attention to also focus on systems development methodologies. The availability of systems development tools, rapid change in technologies, evolution of mobile computing and the growth of cloud computing have necessitated a move toward systems integration and customization rather than developing systems from scratch. This tendency encourages component based development and discourages traditional systems development approach. The paper presents and evaluates SSADM, IDEF0 and Agile systems development methodologies. More specifically, it examines how they fit or not fit into the current competitive market of systems development. In the view of this perspective, it is anticipated that despite of its popularity, SSADM methodology is becoming obsolete while Agile and IDEF0 methodologies are still gaining acceptance in the current competitive market of systems development. The present study more likely enrich our understanding of the systems development methodologies concepts and draw attention regarding where the current trends in system development are heading.

Use of the temporal median and trimmed mean mitigates effects of respiratory motion in multiple-acquisition abdominal diffusion imaging

International Nuclear Information System (INIS)

Jerome, N P; Orton, M R; D’Arcy, J A; Leach, M O; Collins, D J; Feiweier, T; Tunariu, N; Koh, D-M

2015-01-01

Respiratory motion commonly confounds abdominal diffusion-weighted magnetic resonance imaging, where averaging of successive samples at different parts of the respiratory cycle, performed in the scanner, manifests the motion as blurring of tissue boundaries and structural features and can introduce bias into calculated diffusion metrics. Storing multiple averages separately allows processing using metrics other than the mean; in this prospective volunteer study, median and trimmed mean values of signal intensity for each voxel over repeated averages and diffusion-weighting directions are shown to give images with sharper tissue boundaries and structural features for moving tissues, while not compromising non-moving structures. Expert visual scoring of derived diffusion maps is significantly higher for the median than for the mean, with modest improvement from the trimmed mean. Diffusion metrics derived from mono- and bi-exponential diffusion models are comparable for non-moving structures, demonstrating a lack of introduced bias from using the median. The use of the median is a simple and computationally inexpensive alternative to complex and expensive registration algorithms, requiring only additional data storage (and no additional scanning time) while returning visually superior images that will facilitate the appropriate placement of regions-of-interest when analysing abdominal diffusion-weighted magnetic resonance images, for assessment of disease characteristics and treatment response. (note)

CI-Miner: A Semantic Methodology to Integrate Scientists, Data and Documents through the Use of Cyber-Infrastructure

Science.gov (United States)

Pinheiro da Silva, P.; CyberShARE Center of Excellence

2011-12-01

Scientists today face the challenge of rethinking the manner in which they document and make available their processes and data in an international cyber-infrastructure of shared resources. Some relevant examples of new scientific practices in the realm of computational and data extraction sciences include: large scale data discovery; data integration; data sharing across distinct scientific domains, systematic management of trust and uncertainty; and comprehensive support for explaining processes and results. This talk introduces CI-Miner - an innovative hands-on, open-source, community-driven methodology to integrate these new scientific practices. It has been developed in collaboration with scientists, with the purpose of capturing, storing and retrieving knowledge about scientific processes and their products, thereby further supporting a new generation of science techniques based on data exploration. CI-Miner uses semantic annotations in the form of W3C Ontology Web Language-based ontologies and Proof Markup Language (PML)-based provenance to represent knowledge. This methodology specializes in general-purpose ontologies, projected into workflow-driven ontologies(WDOs) and into semantic abstract workflows (SAWs). Provenance in PML is CI-Miner's integrative component, which allows scientists to retrieve and reason with the knowledge represented in these new semantic documents. It serves additionally as a platform to share such collected knowledge with the scientific community participating in the international cyber-infrastructure. The integrated semantic documents that are tailored for the use of human epistemic agents may also be utilized by machine epistemic agents, since the documents are based on W3C Resource Description Framework (RDF) notation. This talk is grounded upon interdisciplinary lessons learned through the use of CI-Miner in support of government-funded national and international cyber-infrastructure initiatives in the areas of geo

Use of Vine-Trimming Wastes as Carrier for Amycolatopsis sp. to Produce Vanillin, Vanillyl Alcohol, and Vanillic Acid.

Science.gov (United States)

Castañón-Rodríguez, Juan Francisco; Pérez-Rodríguez, Noelia; de Souza Oliveira, Ricardo Pinheiro; Aguilar-Uscanga, María Guadalupe; Domínguez, José Manuel

2016-10-01

Raw vine-trimming wastes or the solid residues obtained after different fractionation treatments were evaluated for their suitability as Amycolatopsis sp. immobilization carriers during the bioconversion of ferulic acid into valuable phenolic compounds such as vanillin, vanillyl alcohol, and vanillic acid, the main flavor components of vanilla pods. Previously, physical-chemical characteristics of the materials were determined by quantitative acid hydrolysis and water absorption index (WAI), and microbiological characteristics by calculating the cell retention in the carrier (λ). Additionally, micrographics of carrier surface were obtained by field emission-scanning electron microscopy to study the influence of morphological changes during pretreatments in the adhesion of cells immobilized. The results point out that in spite of showing the lowest WAI and intermediate λ, raw material was the most appropriated substrate to conduct the bioconversion, achieving up to 262.9 mg/L phenolic compounds after 24 h, corresponding to 42.9 mg/L vanillin, 115.6 mg/L vanillyl alcohol, and 104.4 mg/L vanillic acid. The results showed the potential of this process to be applied for biotechnological production of vanillin from ferulic acid solutions; however, further studies must be carried out to increase vanillin yield. Additionally, the liquors obtained after treatment of vine-trimming wastes could be assayed to replace synthetic ferulic acid.

Methodology of Integration for Competitive Technical Intelligence with Blue Ocean Strategy: Application to an exotic fruit

Directory of Open Access Journals (Sweden)

Marisela Rodríguez Salvador

2011-12-01

Full Text Available This article presents a new methodology that integrates Competitive Technical Intelligence with Blue Ocean Strategy. We explore new business niches taking advantage of the synergy that both areas offer, developing a model based on cyclic interactions through a process developed in two stages: Understanding opportunity that arise from idea formulation to decision making and strategic development. The validity of our approach (first stage was observed in the evaluation of an exotic fruit, Anacardium Occidentale, in the South of the State of Veracruz, Mexico with the support of the university ITESM, Campus Monterrey. We identified critical factors for success, opportunities and threats. Results confirm the attractiveness of this crop.

Development of a methodology for conducting an integrated HRA/PRA --. Task 1, An assessment of human reliability influences during LP&S conditions PWRs

Energy Technology Data Exchange (ETDEWEB)

Luckas, W.J.; Barriere, M.T.; Brown, W.S. [Brookhaven National Lab., Upton, NY (United States); Wreathall, J. [Wreathall (John) and Co., Dublin, OH (United States); Cooper, S.E. [Science Applications International Corp., McLean, VA (United States)

1993-06-01

During Low Power and Shutdown (LP&S) conditions in a nuclear power plant (i.e., when the reactor is subcritical or at less than 10--15% power), human interactions with the plant`s systems will be more frequent and more direct. Control is typically not mediated by automation, and there are fewer protective systems available. Therefore, an assessment of LP&S related risk should include a greater emphasis on human reliability than such an assessment made for power operation conditions. In order to properly account for the increase in human interaction and thus be able to perform a probabilistic risk assessment (PRA) applicable to operations during LP&S, it is important that a comprehensive human reliability assessment (HRA) methodology be developed and integrated into the LP&S PRA. The tasks comprising the comprehensive HRA methodology development are as follows: (1) identification of the human reliability related influences and associated human actions during LP&S, (2) identification of potentially important LP&S related human actions and appropriate HRA framework and quantification methods, and (3) incorporation and coordination of methodology development with other integrated PRA/HRA efforts. This paper describes the first task, i.e., the assessment of human reliability influences and any associated human actions during LP&S conditions for a pressurized water reactor (PWR).

A methodological study of environmental simulation in architecture and engineering. Integrating daylight and thermal performance across the urban and building scales

DEFF Research Database (Denmark)

Sattrup, Peter Andreas; Strømann-Andersen, Jakob Bjørn

2011-01-01

This study presents a methodological and conceptual framework that allows for the integration and creation of knowledge across professional borders in the field of environmental simulation. The framework has been developed on the basis of interviews with leading international practitioners, key...... in pointing out the need for improving metrics, software and not least the performance of the built environment itself....

MATLAB Stability and Control Toolbox Trim and Static Stability Module

Science.gov (United States)

Kenny, Sean P.; Crespo, Luis

2012-01-01

MATLAB Stability and Control Toolbox (MASCOT) utilizes geometric, aerodynamic, and inertial inputs to calculate air vehicle stability in a variety of critical flight conditions. The code is based on fundamental, non-linear equations of motion and is able to translate results into a qualitative, graphical scale useful to the non-expert. MASCOT was created to provide the conceptual aircraft designer accurate predictions of air vehicle stability and control characteristics. The code takes as input mass property data in the form of an inertia tensor, aerodynamic loading data, and propulsion (i.e. thrust) loading data. Using fundamental nonlinear equations of motion, MASCOT then calculates vehicle trim and static stability data for the desired flight condition(s). Available flight conditions include six horizontal and six landing rotation conditions with varying options for engine out, crosswind, and sideslip, plus three take-off rotation conditions. Results are displayed through a unique graphical interface developed to provide the non-stability and control expert conceptual design engineer a qualitative scale indicating whether the vehicle has acceptable, marginal, or unacceptable static stability characteristics. If desired, the user can also examine the detailed, quantitative results.

Isolation and characterization of non-O157 Shiga toxin-producing Escherichia coli from beef carcasses, cuts and trimmings of abattoirs in Argentina

Science.gov (United States)

Brusa, Victoria; Restovich, Viviana; Galli, Lucía; Teitelbaum, David; Signorini, Marcelo; Brasesco, Hebe; Londero, Alejandra; García, Diego; Padola, Nora Lía; Superno, Valeria; Sanz, Marcelo; Petroli, Sandra; Costa, Magdalena; Bruzzone, Mariana; Sucari, Adriana; Ferreghini, Marcela; Linares, Luciano; Suberbie, Germán; Rodríguez, Ricardo

2017-01-01

Several foods contaminated with Shiga toxin-producing Escherichia coli (STEC) are associated with human diseases. Some countries have established microbiological criteria for non-O157 STEC, thus, the absence of serogroups O26, O45, O103, O104, O111, O121, and O145 in sprouts from the European Union or ground beef and beef trimmings from the United States is mandatory. While in Argentina screening for O26, O103, O111, O145 and O121 in ground beef, ready-to-eat food, sausages and vegetables is mandatory, other countries have zero-tolerance for all STEC in chilled beef. The aim of this study was to provide data on the prevalence of non-O157 STEC isolated from beef processed in eight Argentinean cattle slaughterhouses producing beef for export and local markets, and to know the non-O157 STEC profiles through strain characterization and genotypic analysis. Samples (n = 15,965) from 3,205 beef carcasses, 9,570 cuts and 3,190 trimmings collected between March and September 2014 were processed in pools of five samples each. Pools of samples (n = 3,193) from 641 carcasses, 1,914 cuts and 638 trimming were analyzed for non-O157 STEC isolation according to ISO/CEN 13136:2012. Of these, 37 pools of carcasses (5.8%), 111 pools of cuts (5.8%) and 45 pools of trimmings (7.0%) were positive for non-O157 STEC. STEC strains (n = 200) were isolated from 193 pools of samples. The most prevalent serotypes were O174:H21, O185:H7, O8:H19, O178:H19 and O130:H11, and the most prevalent genotypes were stx2c(vh-b) and stx2a/saa/ehxA. O103:H21 strain was eae-positive and one O178:H19 strain was aggR/aaiC-positive. The prevalence of non-O157 STEC in beef carcasses reported here was low. None of the non-O157 STEC strains isolated corresponded to the non-O157 STEC serotypes and virulence profiles isolated from human cases in Argentina in the same study period. The application of microbiological criteria for each foodstuff should be determined by risk analysis in order to have a stringent

Isolation and characterization of non-O157 Shiga toxin-producing Escherichia coli from beef carcasses, cuts and trimmings of abattoirs in Argentina.

Directory of Open Access Journals (Sweden)

Victoria Brusa

Full Text Available Several foods contaminated with Shiga toxin-producing Escherichia coli (STEC are associated with human diseases. Some countries have established microbiological criteria for non-O157 STEC, thus, the absence of serogroups O26, O45, O103, O104, O111, O121, and O145 in sprouts from the European Union or ground beef and beef trimmings from the United States is mandatory. While in Argentina screening for O26, O103, O111, O145 and O121 in ground beef, ready-to-eat food, sausages and vegetables is mandatory, other countries have zero-tolerance for all STEC in chilled beef. The aim of this study was to provide data on the prevalence of non-O157 STEC isolated from beef processed in eight Argentinean cattle slaughterhouses producing beef for export and local markets, and to know the non-O157 STEC profiles through strain characterization and genotypic analysis. Samples (n = 15,965 from 3,205 beef carcasses, 9,570 cuts and 3,190 trimmings collected between March and September 2014 were processed in pools of five samples each. Pools of samples (n = 3,193 from 641 carcasses, 1,914 cuts and 638 trimming were analyzed for non-O157 STEC isolation according to ISO/CEN 13136:2012. Of these, 37 pools of carcasses (5.8%, 111 pools of cuts (5.8% and 45 pools of trimmings (7.0% were positive for non-O157 STEC. STEC strains (n = 200 were isolated from 193 pools of samples. The most prevalent serotypes were O174:H21, O185:H7, O8:H19, O178:H19 and O130:H11, and the most prevalent genotypes were stx2c(vh-b and stx2a/saa/ehxA. O103:H21 strain was eae-positive and one O178:H19 strain was aggR/aaiC-positive. The prevalence of non-O157 STEC in beef carcasses reported here was low. None of the non-O157 STEC strains isolated corresponded to the non-O157 STEC serotypes and virulence profiles isolated from human cases in Argentina in the same study period. The application of microbiological criteria for each foodstuff should be determined by risk analysis in order to have a

Integrating cost information with health management support system: an enhanced methodology to assess health care quality drivers.

Science.gov (United States)

Kohli, R; Tan, J K; Piontek, F A; Ziege, D E; Groot, H

1999-08-01

Changes in health care delivery, reimbursement schemes, and organizational structure have required health organizations to manage the costs of providing patient care while maintaining high levels of clinical and patient satisfaction outcomes. Today, cost information, clinical outcomes, and patient satisfaction results must become more fully integrated if strategic competitiveness and benefits are to be realized in health management decision making, especially in multi-entity organizational settings. Unfortunately, traditional administrative and financial systems are not well equipped to cater to such information needs. This article presents a framework for the acquisition, generation, analysis, and reporting of cost information with clinical outcomes and patient satisfaction in the context of evolving health management and decision-support system technology. More specifically, the article focuses on an enhanced costing methodology for determining and producing improved, integrated cost-outcomes information. Implementation issues and areas for future research in cost-information management and decision-support domains are also discussed.

Design of Sustainable Blended Products using an Integrated Methodology

DEFF Research Database (Denmark)

Yunus, Nor Alafiza Binti; Gernaey, Krist; Woodley, John

2013-01-01

This paper presents a systematic methodology for designing blended products consisting of three stages; product design, process identification and experimental verification. The product design stage is considered in this paper. The objective of this stage is to screen and select suitable chemicals...... to be used as building blocks in the mixture design, and then to propose the blend formulations that fulfill the desired product attributes. The result is a set of blends that match the constraints, the compositions, values of the target properties and information about their miscibility. The methodology has...... been applied to design several blended products. A case study on design of blended lubricants is highlighted. The objective is to identify blended products that satisfy the product attributes with at least similar or better performance compared to conventional products....

Methodology for inferring initial flaw distribution

International Nuclear Information System (INIS)

Jouris, G.M.; Shaffer, D.H.

1980-01-01

It has been common practice in both deterministic and probabilistic assessment of the integrity of a pressure vessel to assume the presence of a rather large flaw (usually 1/4 the thickness of the vessel wall) in the belt-line region. Although it is highly unlikely that such a large flaw would be present, the assumption is adopted in order to be conservative. A more realistic approach, which can be incorporated in the probabilistic analysis of integrity, is to characterize the depth of a flaw as a random variable and thus allow the probabilities associated with the presence of various size flaws to be reflected in the final estimated probability of vessel failure. This is precisely the motivation for developing the methodology to obtain the distribution of initial flaw depth, which is presented in this paper. It should be mentioned that the methodology developed here is not an end in itself but rather provides an input distribution to be used in a comprehensive integrity assessment. (orig.)

MEAPA - Integrated methodology for alternative energy sources map pin in the State of Para - Brazil; MEAPA - Metodologias integradas para o mapeamento de energias alternativas no Estado do Para

Energy Technology Data Exchange (ETDEWEB)

Monteiro, Claudio; Lopes, J. Pecas; Va, Kowk P.; Herold, Helmut [Instituto de Engenharia de Sistemas e Computadores (INESC), Porto (Portugal). Power System Unit. E-mail: cmonteiro@inescn.pt; Rocha, Brigida; Pinheiro, Helten; Rocha, Olavo [Para Univ., Belem, PA (Brazil). Dept. de Engenharia Eletrica. E-mail: rocha@interconect.com.br; Silva, Isa O. [Para Univ., Belem, PA (Brazil). Dept. de Meteorologia; Moraes, Sinfronio [Para Univ., Belem, PA (Brazil). Dept. de Mecanica

1999-07-01

This paper describes the MEAPA project for the development of methodologies which support the renewable energy sources integration in the Marajo island - Para - Brazil. The methodologies used will be described including the construction of a geographical database, renewable resources (wind, solar and biomass) mapping, transport costs, cost of electric power produced by various systems and electrification sceneries and comparison of electrification solutions.

METODOLOGÍA DE DISEÑO ORGANIZACIONAL INTEGRANDO ENFOQUE A PROCESOS Y COMPETENCIAS / METHODOLOGY OF ORGANIZATIONAL DESIGN INTEGRATING PROCESS APPROACH AND COMPETENCIES

Directory of Open Access Journals (Sweden)

Iraida Justina Rodríguez-González

2012-04-01

Full Text Available La Metodología de Diseño Organizacional responde a la introducción de formas organizativas basadas en el diseño de procesos, la estrategia y la gestión por competencias; integradas con los sistemas y tecnologías de la información. El modelo de diseño tiene como objetivo fundamental brindar una herramienta que permita diseñar una organización de nueva creación al tiempo que se va construyendo, sustentado en una plataforma integrada. La aplicación realizada en el Complejo de Investigaciones Tecnológicas Integradas (CITI demuestra la factibilidad de la metodología en las condiciones actuales y los resultados obtenidos avalan los procedimientos que la hacen funcional para Cuba y adaptable a objetivos similares en otros países.AbstractThe Methodology of Organizational Design corresponds to the introduction of organizational forms based on process design, strategy and management by competencies, which are integrated with the systems and information technologies. The design model aims to provide a tool to design a new organization while it is constructed, based on an integrated platform. The application carried out in the Complex of Integrated Technological Investigations (CITI, in its Spanish acronym, demonstrates the feasibility of the methodology under the current conditions and the obtained results endorse the procedures that make it functional for Cuba and adaptable to similar objectives in other countries.

Using a Realist Research Methodology in Policy Analysis

Science.gov (United States)

Lourie, Megan; Rata, Elizabeth

2017-01-01

The article describes the usefulness of a realist methodology in linking sociological theory to empirically obtained data through the development of a methodological device. Three layers of analysis were integrated: 1. the findings from a case study about Maori language education in New Zealand; 2. the identification and analysis of contradictions…

Elastic-Plastic J-Integral Solutions or Surface Cracks in Tension Using an Interpolation Methodology

Science.gov (United States)

Allen, P. A.; Wells, D. N.

2013-01-01

No closed form solutions exist for the elastic-plastic J-integral for surface cracks due to the nonlinear, three-dimensional nature of the problem. Traditionally, each surface crack must be analyzed with a unique and time-consuming nonlinear finite element analysis. To overcome this shortcoming, the authors have developed and analyzed an array of 600 3D nonlinear finite element models for surface cracks in flat plates under tension loading. The solution space covers a wide range of crack shapes and depths (shape: 0.2 less than or equal to a/c less than or equal to 1, depth: 0.2 less than or equal to a/B less than or equal to 0.8) and material flow properties (elastic modulus-to-yield ratio: 100 less than or equal to E/ys less than or equal to 1,000, and hardening: 3 less than or equal to n less than or equal to 20). The authors have developed a methodology for interpolating between the goemetric and material property variables that allows the user to reliably evaluate the full elastic-plastic J-integral and force versus crack mouth opening displacement solution; thus, a solution can be obtained very rapidly by users without elastic-plastic fracture mechanics modeling experience. Complete solutions for the 600 models and 25 additional benchmark models are provided in tabular format.

A hybrid design methodology for structuring an Integrated Environmental Management System (IEMS) for shipping business.

Science.gov (United States)

Celik, Metin

2009-03-01

The International Safety Management (ISM) Code defines a broad framework for the safe management and operation of merchant ships, maintaining high standards of safety and environmental protection. On the other hand, ISO 14001:2004 provides a generic, worldwide environmental management standard that has been utilized by several industries. Both the ISM Code and ISO 14001:2004 have the practical goal of establishing a sustainable Integrated Environmental Management System (IEMS) for shipping businesses. This paper presents a hybrid design methodology that shows how requirements from both standards can be combined into a single execution scheme. Specifically, the Analytic Hierarchy Process (AHP) and Fuzzy Axiomatic Design (FAD) are used to structure an IEMS for ship management companies. This research provides decision aid to maritime executives in order to enhance the environmental performance in the shipping industry.

Nursing home staff members' subjective frames of reference on residents' achievement of ego integrity: A Q-methodology study.

Science.gov (United States)

Lim, Sun-Young; Chang, Sung-Ok

2018-01-01

To discover the structure of the frames of reference for nursing home staff members' subjective judgment of residents' achievement of ego integrity. Q-methodology was applied. Twenty-eight staff members who were working in a nursing home sorted 34 Q-statements into the shape of a normal distribution. A centroid factor analysis and varimax rotation, using the PQ-method program, revealed four factors: identifying clues to residents' positive acceptance of their whole life span, identifying residents' ways of enjoying their current life, referencing residents' attitudes and competencies toward harmonious relationships, and identifying residents' integrated efforts to establish self-esteem. These subjective frames of reference need to be investigated in order to improve the relationships with nursing home residents and their quality of life. Consequently, the fundamental monitoring tools to help staff members make subjective judgments can be formed. © 2017 Japan Academy of Nursing Science.

Transboundary Water: Improving Methodologies and Developing Integrated Tools to Support Water Security

Science.gov (United States)

Hakimdavar, Raha; Wood, Danielle; Eylander, John; Peters-Lidard, Christa; Smith, Jane; Doorn, Brad; Green, David; Hummel, Corey; Moore, Thomas C.

2018-01-01

River basins for which transboundary coordination and governance is a factor are of concern to US national security, yet there is often a lack of sufficient data-driven information available at the needed time horizons to inform transboundary water decision-making for the intelligence, defense, and foreign policy communities. To address this need, a two-day workshop entitled Transboundary Water: Improving Methodologies and Developing Integrated Tools to Support Global Water Security was held in August 2017 in Maryland. The committee that organized and convened the workshop (the Organizing Committee) included representatives from the National Aeronautics and Space Administration (NASA), the US Army Corps of Engineers Engineer Research and Development Center (ERDC), and the US Air Force. The primary goal of the workshop was to advance knowledge on the current US Government and partners' technical information needs and gaps to support national security interests in relation to transboundary water. The workshop also aimed to identify avenues for greater communication and collaboration among the scientific, intelligence, defense, and foreign policy communities. The discussion around transboundary water was considered in the context of the greater global water challenges facing US national security.

Generation of a human immunodeficiency virus type 1 chronically infected monkey B cell line expressing low levels of endogenous TRIM5alpha.

Science.gov (United States)

Ridolfi, Barbara; Catone, Stefania; Sgarbanti, Marco; Sernicola, Leonardo; Battistini, Angela; Parolin, Cristina; Titti, Fausto; Borsetti, Alessandra

2009-12-01

Several innate cellular antiviral factors exist in mammalian cells that prevent the replication of retroviruses. Among them, the tripartite motif protein (TRIM)5alpha has been shown to block human immunodeficiency virus type 1 (HIV-1) infection in several types of Old World monkey cells. Here we report a novel HIV-1 chronically infected monkey B cell line, F6/HIV-1, characterized by very low levels of TRIM5alpha expression that allows HIV-1 to overcome the restriction. Virus produced by F6/HIV-1 cells fails to infect monkey cells but retains the ability to infect human peripheral blood mononuclear cells (PBMCs) and T cell lines, although with a reduced infectivity compared to the input virus. Ultrastructural analyses revealed the presence of budding virions at the F6/HIV-1 cells plasma membrane characterized by a typical conical core shell. To our knowledge F6/HIV-1 is the first monkey cell line chronically infected by HIV-1 and able to release infectious particles thus representing a useful tool to gain further insights into the molecular mechanisms of HIV-1 pathogenesis.

A Physics-Based Engineering Methodology for Calculating Soft Error Rates of Bulk CMOS and SiGe Heterojunction Bipolar Transistor Integrated Circuits

Science.gov (United States)

Fulkerson, David E.

2010-02-01

This paper describes a new methodology for characterizing the electrical behavior and soft error rate (SER) of CMOS and SiGe HBT integrated circuits that are struck by ions. A typical engineering design problem is to calculate the SER of a critical path that commonly includes several circuits such as an input buffer, several logic gates, logic storage, clock tree circuitry, and an output buffer. Using multiple 3D TCAD simulations to solve this problem is too costly and time-consuming for general engineering use. The new and simple methodology handles the problem with ease by simple SPICE simulations. The methodology accurately predicts the measured threshold linear energy transfer (LET) of a bulk CMOS SRAM. It solves for circuit currents and voltage spikes that are close to those predicted by expensive 3D TCAD simulations. It accurately predicts the measured event cross-section vs. LET curve of an experimental SiGe HBT flip-flop. The experimental cross section vs. frequency behavior and other subtle effects are also accurately predicted.

Interrelationships between man, energy, and water quality: a new methodology for integrative analyses

International Nuclear Information System (INIS)

Kaplan, E.; Thode, H.C. Jr.

1979-01-01

The STORET/MSP option was used to obtain county aggregated information on ambient water quality for sixty parameters during the period 1950 to 1978. Masks, extended EXTRACT specifications and bounds on allowable values limited inclusion of erroneous data. Remark codes were required to aggregate STORET parameters to obtain increased numbers of observations. Numerous statistical analyses led to the conclusions that medians were more useful than means, that trimming on number of observations was required to eliminate counties with extreme values, and that many parameters required logarithmic transformation to be useful in regional analyses. County aggregated data for nineteen water quality parameters were examined in terms of their ability to describe qualitative chemical characteristics of water. Anion--cation balances as well as expected relationships between conductivity and other parameters were correctly accounted for. Factor analysis indicated the existence of three principal components describing patterns between metal ions, non-metal ions, and alkalinity-bicarbonate, respectively. These factors were used in place of the original complete set of water quality parameters in a structural equation approach describing relationships between variables of mans activites. It was found that counties with high industrial electric consumption, farming and mineral shipments tended to have increased levels of most water quality parameters. It was also found that simpler path diagrams may be indicated to reduce the effects of redundancy in adequately describing energy--water relationships

Technical Report on Preliminary Methodology for Enhancing Risk Monitors with Integrated Equipment Condition Assessment

Energy Technology Data Exchange (ETDEWEB)

Ramuhalli, Pradeep; Coles, Garill A.; Coble, Jamie B.; Hirt, Evelyn H.

2013-09-17

Small modular reactors (SMRs) generally include reactors with electric output of ~350 MWe or less (this cutoff varies somewhat but is substantially less than full-size plant output of 700 MWe or more). Advanced SMRs (AdvSMRs) refer to a specific class of SMRs and are based on modularization of advanced reactor concepts. AdvSMRs may provide a longer-term alternative to traditional light-water reactors (LWRs) and SMRs based on integral pressurized water reactor concepts currently being considered. Enhancing affordability of AdvSMRs will be critical to ensuring wider deployment. AdvSMRs suffer from loss of economies of scale inherent in small reactors when compared to large (~greater than 600 MWe output) reactors. Some of this loss can be recovered through reduced capital costs through smaller size, fewer components, modular fabrication processes, and the opportunity for modular construction. However, the controllable day-to-day costs of AdvSMRs will be dominated by operation and maintenance (O&M) costs. Technologies that help characterize real-time risk are important for controlling O&M costs. Risk monitors are used in current nuclear power plants to provide a point-in-time estimate of the system risk given the current plant configuration (e.g., equipment availability, operational regime, and environmental conditions). However, current risk monitors are unable to support the capability requirements listed above as they do not take into account plant-specific normal, abnormal, and deteriorating states of active components and systems. This report documents technology developments that are a step towards enhancing risk monitors that, if integrated with supervisory plant control systems, can provide the capability requirements listed and meet the goals of controlling O&M costs. The report describes research results from an initial methodology for enhanced risk monitors by integrating real-time information about equipment condition and POF into risk monitors.

Integrated security system definition

International Nuclear Information System (INIS)

Campbell, G.K.; Hall, J.R. II

1985-01-01

The objectives of an integrated security system are to detect intruders and unauthorized activities with a high degree of reliability and the to deter and delay them until effective response/engagement can be accomplished. Definition of an effective integrated security system requires proper application of a system engineering methodology. This paper summarizes a methodology and describes its application to the problem of integrated security system definition. This process includes requirements identification and analysis, allocation of identified system requirements to the subsystem level and provides a basis for identification of synergistic subsystem elements and for synthesis into an integrated system. The paper discusses how this is accomplished, emphasizing at each step how system integration and subsystem synergism is considered. The paper concludes with the product of the process: implementation of an integrated security system

Dynamic event Tress applied to sequences Full Spectrum LOCA. Calculating the frequency of excedeence of damage by integrated Safety Analysis Methodology

International Nuclear Information System (INIS)

Gomez-Magan, J. J.; Fernandez, I.; Gil, J.; Marrao, H.; Queral, C.; Gonzalez-Cadelo, J.; Montero-Mayorga, J.; Rivas, J.; Ibane-Llano, C.; Izquierdo, J. M.; Sanchez-Perea, M.; Melendez, E.; Hortal, J.

2013-01-01

The Integrated Safety Analysis (ISA) methodology, developed by the Spanish Nuclear Safety council (CSN), has been applied to obtain the dynamic Event Trees (DETs) for full spectrum Loss of Coolant Accidents (LOCAs) of a Westinghouse 3-loop PWR plant. The purpose of this ISA application is to obtain the Damage Excedence Frequency (DEF) for the LOCA Event Tree by taking into account the uncertainties in the break area and the operator actuation time needed to cool down and de pressurize reactor coolant system by means of steam generator. Simulations are performed with SCAIS, a software tool which includes a dynamic coupling with MAAP thermal hydraulic code. The results show the capability of the ISA methodology to obtain the DEF taking into account the time uncertainty in human actions. (Author)

M&A Performance and Economic Impact: Integration and Critical Assessment of Methodological Approach

Directory of Open Access Journals (Sweden)

Karolis Andriuskevicius

2017-11-01

Full Text Available Purpose of the article: Existing methodologies employed within the M&A performance framework are investigated and critically discuss. Methodology/methods: The research has been carried out as a structured assessment of past literature. The findings from scientific articles and studies by various scholars have been categorized, grouped and summarized to discern a meta-analytic view of the work carried out to date. Scientific aim: The conducted research seeks to ascertain and evaluate theoretically existing methodologies used in empirical studies that would allow proper and critical understanding of the results of various findings in the holistic and global M&As area. Findings: The research elaborates on several key developments in M&A methodology and performance studies carried out in empirical works during the last two decades. The findings help to independently and objectively assess performance of M&A from a holistic perspective. Conclusions: Each methodology measuring either M&A performance on a corporate level or effects of M&A on the economy level shall be interpreted and relied on with caution as each of them dispose their limitations whereas application of these methodologies is subject to data availability and case specific.

An integrated methodology to forecast the efficiency of nourishment strategies in eroding deltas.

Science.gov (United States)

Bergillos, Rafael J; López-Ruiz, Alejandro; Principal-Gómez, Daniel; Ortega-Sánchez, Miguel

2018-02-01

Many deltas across the globe are retreating, and nearby beaches are undergoing strong erosion as a result. Among soft and prompt solutions, nourishments are the most heavily used. This paper presents an integrated methodology to forecast the efficiency of nourishment strategies by means of wave climate simulations, wave propagations with downscaling techniques, computation of longshore sediment transport rates and application of the one-line model. It was applied to an eroding deltaic beach (Guadalfeo, southern Spain), where different scenarios as a function of the nourished coastline morphology, input volume and grain size were tested. For that, the evolution of six scenarios of coastline geometry over a two-year period (lifetime of nourishment projects at the study site) was modelled and the uncertainty of the predictions was also quantified through Monte Carlo techniques. For the most efficient coastline shape in terms of gained dry beach area, eight sub-scenarios with different nourished volumes were defined and modelled. The results indicate that an input volume around 460,000m 3 is the best strategy since nourished morphologies with higher volumes are more exposed to the prevailing storm directions, inducing less efficient responses. After setting the optimum coastline morphology and input sediment volume, eleven different nourished grain sizes were modelled; the most efficient coastline responses were obtained for sediment sizes greater than 0.01m. The availability of these sizes in the sediment accumulated upstream of a dam in the Guadalfeo River basin allows for the conclusion that this alternative would not only mitigate coastal erosion problems but also sedimentation issues in the reservoir. The methodology proposed in this work is extensible to other coastal areas across the world and can be helpful to support the decision-making process of artificial nourishment projects and other environmental management strategies. Copyright © 2017 Elsevier B.V. All

Environmental Zoning: Some methodological implications

NARCIS (Netherlands)

Ike, Paul; Voogd, Henk

1991-01-01

The purpose of this article is to discuss some methodological problems of environmental zoning. The principle of environmental zoning will be elaborated. In addition an overview is given of a number of approaches that have been followed in practice to arrive at an integral judgement. Finally some

Hanford Site baseline risk assessment methodology

International Nuclear Information System (INIS)

1993-03-01

This methodology has been developed to prepare human health and environmental evaluations of risk as part of the Comprehensive Environmental Response, Compensation, and Liability Act remedial investigations (RIs) and the Resource Conservation and Recovery Act facility investigations (FIs) performed at the Hanford Site pursuant to the Hanford Federal Facility Agreement and Consent Order referred to as the Tri-Party Agreement. Development of the methodology has been undertaken so that Hanford Site risk assessments are consistent with current regulations and guidance, while providing direction on flexible, ambiguous, or undefined aspects of the guidance. The methodology identifies Site-specific risk assessment considerations and integrates them with approaches for evaluating human and environmental risk that can be factored into the risk assessment program supporting the Hanford Site cleanup mission. Consequently, the methodology will enhance the preparation and review of individual risk assessments at the Hanford Site

Effects of Inlet Modification and Rocket-Rack Extension on the Longitudinal Trim and Low-Lift Drag of the Douglas F5D-1 Airplane as Obtained with a 0.125-Scale Rocket-Boosted Model Between Mach Numbers of 0.81 and 1.64: TED No. NACA AD 399

Science.gov (United States)

Hastings, Earl C., Jr.; Dickens, Waldo L.

1957-01-01

A flight investigation was conducted to determine the effects of inlet modification and rocket-rack extension on the longitudinal trim and low-lift drag of the Douglas F5D-1 airplane. The investigation was conducted with a 0.125-scale rocket-boosted model between Mach Numbers of 0.81 and 1.64. This paper presents the changes in trim angle of attack, trim lift coefficient, and low-lift drag caused by the modified inlets alone over a small part of the test Mach number range and by a combination of the modified inlets and extended rocket racks throughout the remainder of the test.

Methodological study of the adjustment of multigroup cross sections for fast reactors, by means of integral data

International Nuclear Information System (INIS)

Bastos, H.F.B.N.

1979-01-01

In this work a study of the methodology of the adjustment of multigroup cross sections by means of integral data is presented. A synthesis of the principal methods existent and the mathematical development of the adaptation of one of them are made. A calculational system is built from this reference method, with the basic conditions for the operation of the process of adjustment. In order to test the system developed and analyze several problems related to the adjustment, a series of trial adjustments was made with the value of the U 235 fission cross section from the infinite dilution library used in the calculational system for fast reactors of the Instituto de Engenharia Nuclear. (author)

Development of an integrated methodology for the sustainable environmental and socio-economic management of river ecosystems.

Science.gov (United States)

Koundouri, P; Ker Rault, P; Pergamalis, V; Skianis, V; Souliotis, I

2016-01-01

The development of the Water Framework Directive aimed to establish an integrated framework of water management at European level. This framework revolves around inland surface waters, transitional waters, coastal waters and ground waters. In the process of achieving the environment and ecological objectives set from the Directive, the role of economics is put in the core of the water management. An important feature of the Directive is the recovery of total economic cost of water services by all users. The total cost of water services can be disaggregated into environmental, financial and resource costs. Another important aspect of the directive is the identification of major drivers and pressures in each River Basin District. We describe a methodology that is aiming to achieve sustainable and environmental and socioeconomic management of freshwater ecosystem services. The Ecosystem Services Approach is in the core of the suggested methodology for the implementation of a more sustainable and efficient water management. This approach consists of the following three steps: (i) socio-economic characterization of the River Basin area, (ii) assessment of the current recovery of water use cost, and (iii) identification and suggestion of appropriate programs of measures for sustainable water management over space and time. This methodology is consistent with a) the economic principles adopted explicitly by the Water Framework Directive (WFD), b) the three-step WFD implementation approach adopted in the WATECO document, c) the Ecosystem Services Approach to valuing freshwater goods and services to humans. Furthermore, we analyze how the effects of multiple stressors and socio-economic development can be quantified in the context of freshwater resources management. We also attempt to estimate the value of four ecosystem services using the benefit transfer approach for the Anglian River Basin, which showed the significance of such services. Copyright © 2015. Published by

Application of Master Curve Methodology for Structural Integrity Assessments of Nuclear Components

Energy Technology Data Exchange (ETDEWEB)

Sattari-Far, Iradj [Det Norske Veritas, Stockholm (Sweden); Wallin, Kim [VTT, Esbo (Finland)

2005-10-15

The objective was to perform an in-depth investigation of the Master Curve methodology and also based on this method develop a procedure for fracture assessments of nuclear components. The project has sufficiently illustrated the capabilities of the Master Curve methodology for fracture assessments of nuclear components. Within the scope of this work, the theoretical background of the methodology and its validation on small and large specimens has been studied and presented to a sufficiently large extent, as well as the correlations between the charpy-V data and the Master Curve T{sub 0} reference temperature in the evaluation of fracture toughness. The work gives a comprehensive report of the background theory and the different applications of the Master Curve methodology. The main results of the work have shown that the cleavage fracture toughness is characterized by a large amount of statistical scatter in the transition region, it is specimen size dependent and it should be treated statistically rather than deterministically. The Master Curve methodology is able to make use of statistical data in a consistent way. Furthermore, the Master Curve methodology provides a more precise prediction of the fracture toughness of embrittled materials in comparison with the ASME K{sub IC} reference curve, which often gives over-conservative results. The suggested procedure in this study, concerning the application of the Master Curve method in fracture assessments of ferritic steels in the transition region and the low shelf regions, is valid for the temperatures range T{sub 0}-50{<=}T{<=}T{sub 0}+50 deg C. If only approximate information is required, the Master Curve may well be extrapolated outside this temperature range. The suggested procedure has also been illustrated for some examples.

Mechanisms for plasma etching of HfO{sub 2} gate stacks with Si selectivity and photoresist trimming

Energy Technology Data Exchange (ETDEWEB)

Shoeb, Juline; Kushner, Mark J. [Department of Electrical and Computer Engineering, Iowa State University, Ames, Iowa 50011 (United States); Department of Electrical Engineering and Computer Science, University of Michigan, Ann Arbor, Michigan 48109-2122 (United States)

2009-11-15

To minimize leakage currents resulting from the thinning of the insulator in the gate stack of field effect transistors, high-dielectric constant (high-k) metal oxides, and HfO{sub 2} in particular, are being implemented as a replacement for SiO{sub 2}. To speed the rate of processing, it is desirable to etch the gate stack (e.g., metal gate, antireflection layers, and dielectric) in a single process while having selectivity to the underlying Si. Plasma etching using Ar/BCl{sub 3}/Cl{sub 2} mixtures effectively etches HfO{sub 2} while having good selectivity to Si. In this article, results from integrated reactor and feature scale modeling of gate-stack etching in Ar/BCl{sub 3}/Cl{sub 2} plasmas, preceded by photoresist trimming in Ar/O{sub 2} plasmas, are discussed. It was found that BCl{sub n} species react with HfO{sub 2}, which under ion impact, form volatile etch products such as B{sub m}OCl{sub n} and HfCl{sub n}. Selectivity to Si is achieved by creating Si-B bonding as a precursor to the deposition of a BCl{sub n} polymer which slows the etch rate relative to HfO{sub 2}. The low ion energies required to achieve this selectivity then challenge one to obtain highly anisotropic profiles in the metal gate portion of the stack. Validation was performed with data from literature. The effect of bias voltage and key reactant probabilities on etch rate, selectivity, and profile are discussed.

An Integrated Thermal Compensation System for MEMS Inertial Sensors

Directory of Open Access Journals (Sweden)

Sheng-Ren Chiu

2014-03-01

Full Text Available An active thermal compensation system for a low temperature-bias-drift (TBD MEMS-based gyroscope is proposed in this study. First, a micro-gyroscope is fabricated by a high-aspect-ratio silicon-on-glass (SOG process and vacuum packaged by glass frit bonding. Moreover, a drive/readout ASIC, implemented by the 0.25 µm 1P5M standard CMOS process, is designed and integrated with the gyroscope by directly wire bonding. Then, since the temperature effect is one of the critical issues in the high performance gyroscope applications, the temperature-dependent characteristics of the micro-gyroscope are discussed. Furthermore, to compensate the TBD of the micro-gyroscope, a thermal compensation system is proposed and integrated in the aforementioned ASIC to actively tune the parameters in the digital trimming mechanism, which is designed in the readout ASIC. Finally, some experimental results demonstrate that the TBD of the micro-gyroscope can be compensated effectively by the proposed compensation system.

The TE coupled RELAP5/PANTHER/COBRA code package and methodology for integrated PWR accident analysis

International Nuclear Information System (INIS)

Schneidesch, Christophe R.; Zhang, Jinzhao; Ammirabile, Luca; Dalleur, Jean-Paul

2006-01-01

coupled thermal hydraulic-neutronic transient calculations allow realistic simulations and hence better understanding the key physical phenomena. The TE coupled code package will be applied to develop coupled analysis methodologies for integrated safety analysis of other PWR accidents. (authors)

The TE coupled RELAP5/PANTHER/COBRA code package and methodology for integrated PWR accident analysis

Energy Technology Data Exchange (ETDEWEB)

Schneidesch, Christophe R.; Zhang, Jinzhao; Ammirabile, Luca; Dalleur, Jean-Paul [Suez-Tractebel Engineering, Avenue Ariane 7, B-1200 Brussels (Belgium)

2006-07-01

coupled thermal hydraulic-neutronic transient calculations allow realistic simulations and hence better understanding the key physical phenomena. The TE coupled code package will be applied to develop coupled analysis methodologies for integrated safety analysis of other PWR accidents. (authors)

Methodological exemplar of integrating quantitative and qualitative evidence - supportive care for men with prostate cancer:what are the most important components?

OpenAIRE

Huntley, Alyson; King, Anna J L; Moore, Theresa H M; Paterson, Charlotte; Persad, Raj; Sharp, Debbie J; Evans, Maggie A

2017-01-01

AIMS: To present a methodological exemplar of integrating findings from a quantitative and qualitative review on the same topic to provide insight into components of care that contribute to supportive care that is acceptable to men with prostate cancer.BACKGROUND: Men with prostate cancer are likely to live a long time with the disease, experience side effects from treatment and therefore have ongoing supportive care needs. Quantitative and qualitative reviews have been published but the find...

Influência de métodos de debicagem e do tipo de bebedouro no desempenho e na qualidade dos ovos de codornas japonesas Beak trimming method and drinking system and a their effect on the performance and egg quality of japanese quails

Directory of Open Access Journals (Sweden)

Christine Laganá

2011-06-01

(non-trimmed birds; 1/3 of the beak trimmed and cauterized beak, 8 replicates of 28 birds to evaluate the effect of beak trimming and drinking system on productive performance. The birds were beak trimmed at 21 days of age, and at day 35 the weight gain, feed intake, feed conversion and mortality were evaluated. At day 39, 576 quails were transfered to laying cages, and assigned to a 3x2 factorial arrangement, to evaluate three beak trimming methods and two drinking systems (cup and nipple by using four replicates of 24 birds each. The production phase started when the birds reached 50% egg production, at 60 days of age. The quails submitted to beak trimming by cauterization showed smaller weight and lower feed intake during the initial period. Stress pain due to the cauterized beak can justify not only lower feed intake and lower weight gain, but also increased mortality. It can be inferred that once this method takes more time it has caused more pain and higher levels of stress, worsening the performance of birds. The trimmed to 1/3 of the beaks cut can have caused stress to the birds less intense, without effect on performance. No differences on bird performance and egg quality were observed between drinking systems and beak trimming during the laying phase. In the initial phase, beak trimming system reduced performance, however, during the production phase, both drinking and beak trimming systems do not affect performance and egg quality of Japanese quails.

Covariance Evaluation Methodology for Neutron Cross Sections

Energy Technology Data Exchange (ETDEWEB)

Herman,M.; Arcilla, R.; Mattoon, C.M.; Mughabghab, S.F.; Oblozinsky, P.; Pigni, M.; Pritychenko, b.; Songzoni, A.A.

2008-09-01

We present the NNDC-BNL methodology for estimating neutron cross section covariances in thermal, resolved resonance, unresolved resonance and fast neutron regions. The three key elements of the methodology are Atlas of Neutron Resonances, nuclear reaction code EMPIRE, and the Bayesian code implementing Kalman filter concept. The covariance data processing, visualization and distribution capabilities are integral components of the NNDC methodology. We illustrate its application on examples including relatively detailed evaluation of covariances for two individual nuclei and massive production of simple covariance estimates for 307 materials. Certain peculiarities regarding evaluation of covariances for resolved resonances and the consistency between resonance parameter uncertainties and thermal cross section uncertainties are also discussed.

[Quality and integrity in the production and publication of scientific results: data trimming, manipulation and (self) plagiarism].

Science.gov (United States)

Lüscher, T F

2014-08-01

The publication of scientific manuscripts is an essential part in the research process and in the attempt to produce novel knowledge: only what is published exists. It is the aim of research to produce reproducible and sustainable knowledge. Reproducible knowledge is based on precise observation, the use of modern methodologies and an appropriate statistical analysis. As a consequence, it must be the intention of any scientist to report the truth and nothing but the truth. This principle requires precision and honesty. Deviation from such a behavior may lead to scientific misconduct: It encompasses the use of inappropriate methods and/or statistics, double publication of data, sloppy data presentation and processing, up to data massaging, manipulation, data theft or fabrication. Famous examples can be found throughout the history of research but it appears that such behavior has recently become more common possibly due to excessive competition, the crucial role of grants for scientific productivity and funding as well as promotion. Accordingly, in the training of researchers it seems essential to emphasize the importance of precise data acquisition and analysis to ascertain reproducible data. Similarly, it must be assured that data sets are only published once, that authors have contributed technically and/or intellectually in an important manner and that the work of other scientists is cited appropriately. Editors and reviewers should carefully assess the quality of submitted manuscripts. In fact, it is the aim of the peer review process to assure as much as possible that the quality of submitted manuscripts meets current methodological as well as ethical standards.

Methodology for the knowledge preservation in the CSN Proyecto RECO

Energy Technology Data Exchange (ETDEWEB)

Montero, M.A.; Gil, E.; Merino, C.; Gonzalez, N.

2016-07-01

This paper summarizes the RECOR Project carried out by the Spanish Nuclear Regulatory Authority (CSN) to develop a methodology to preserve nuclear knowledge, specifically adapted to the CSN regulatory functions. The project covers the development, implantation and verification of the methodology and the development necessary procedures and tools to align it with the CSN Integrated Management System. The Project is complemented with a number of sessions to motivate the CSN management with the methodology and training the staff in the use and Implementation of the methodology. (Author)

Uma abordagem metodológica para o desenvolvimento de sistemas automatizados e integrados de manufatura A methodological approach to automated and integrated manufacturing systems development

Directory of Open Access Journals (Sweden)

Marco Antonio Busetti de Paula

2008-01-01

Full Text Available Este trabalho apresenta uma metodologia de projeto aplicada a sistemas automatizados e integrados de manufatura. A metodologia consiste em um desenvolvimento cíclico de três etapas - modelagem, síntese e implementação - até o atendimento da aplicação demandada para o sistema real, resultando no projeto do sistema automatizado integrado. Esta forma de desenvolvimento permite uma revisão contínua dos resultados obtidos em cada etapa. Para testar e validar a metodologia, é apresentado um exemplo de re-projeto de um protótipo de sistema de manufatura em função da necessidade de inserção de um novo produto.This paper presents a methodology of project applied to automated and integrated manufacturing systems. The methodology consists of a cyclic three stages development - modeling, synthesis and implementation - till the accomplishment of the application required by the real system, resulting in the project of the automated and integrated system. This kind of development allows a continuous revision of the results of each stage. To submit to a test and to validate the methodology, it is given an example of a re-project of a prototype of a manufacturing system at the time of the introduction of a new product on the market.

IMSF: Infinite Methodology Set Framework

Science.gov (United States)

Ota, Martin; Jelínek, Ivan

Software development is usually an integration task in enterprise environment - few software applications work autonomously now. It is usually a collaboration of heterogeneous and unstable teams. One serious problem is lack of resources, a popular result being outsourcing, ‘body shopping’, and indirectly team and team member fluctuation. Outsourced sub-deliveries easily become black boxes with no clear development method used, which has a negative impact on supportability. Such environments then often face the problems of quality assurance and enterprise know-how management. The used methodology is one of the key factors. Each methodology was created as a generalization of a number of solved projects, and each methodology is thus more or less connected with a set of task types. When the task type is not suitable, it causes problems that usually result in an undocumented ad-hoc solution. This was the motivation behind formalizing a simple process for collaborative software engineering. Infinite Methodology Set Framework (IMSF) defines the ICT business process of adaptive use of methods for classified types of tasks. The article introduces IMSF and briefly comments its meta-model.

Integrated assessment of the global warming problem. A decision-analytical approach

International Nuclear Information System (INIS)

Van Lenthe, J.; Hendrickx, L.; Vlek, C.A.J.

1995-01-01

The project on the title subject aims at developing a policy-oriented methodology for the integrated assessment of the global warming problem. Decision analysis in general and influence diagrams in particular appear to constitute an appropriate integrated assessment methodology. The influence-diagram approach is illustrated at a preliminary integrated modeling of the global warming problem. In next stages of the research, attention will be shifted from the methodology of integrated assessment to the contents of integrated models. 4 figs., 5 refs

An Updated Methodology for Enhancing Risk Monitors with Integrated Equipment Condition Assessment

Energy Technology Data Exchange (ETDEWEB)

Ramuhalli, Pradeep [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Hirt, Evelyn H. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Coles, Garill A. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Bonebrake, Christopher A. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Ivans, William J. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Wootan, David W. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Mitchell, Mark R. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States)

2014-07-18

Small modular reactors (SMRs) generally include reactors with electric output of ~350 MWe or less (this cutoff varies somewhat but is substantially less than full-size plant output of 700 MWe or more). Advanced SMRs (AdvSMRs) refer to a specific class of SMRs and are based on modularization of advanced reactor concepts. Enhancing affordability of AdvSMRs will be critical to ensuring wider deployment, as AdvSMRs suffer from loss of economies of scale inherent in small reactors when compared to large (~greater than 600 MWe output) reactors and the controllable day-to-day costs of AdvSMRs will be dominated by operation and maintenance (O&M) costs. Technologies that help characterize real-time risk are important for controlling O&M costs. Risk monitors are used in current nuclear power plants to provide a point-in-time estimate of the system risk given the current plant configuration (e.g., equipment availability, operational regime, and environmental conditions). However, current risk monitors are unable to support the capability requirements listed above as they do not take into account plant-specific normal, abnormal, and deteriorating states of active components and systems. This report documents technology developments towards enhancing risk monitors that, if integrated with supervisory plant control systems, can provide the capability requirements listed and meet the goals of controlling O&M costs. The report describes research results on augmenting an initial methodology for enhanced risk monitors that integrate real-time information about equipment condition and POF into risk monitors. Methods to propagate uncertainty through the enhanced risk monitor are evaluated. Available data to quantify the level of uncertainty and the POF of key components are examined for their relevance, and a status update of this data evaluation is described. Finally, we describe potential targets for developing new risk metrics that may be useful for studying trade-offs for economic

The Potential Role of Mental Model Methodologies in Multistakeholder Negotiations: Integrated Water Resources Management in South Africa

Directory of Open Access Journals (Sweden)

Derick R. Du Toit

2011-09-01

Full Text Available Equitable redistribution of resources is an emergent phenomenon in democratizing countries, and attempts are often characterized by decentralized decision making within a framework of multistakeholder negotiations. South Africa offers a unique opportunity to explore the manifestations of these relationships, particularly through Integrated Water Resources Management and its National Water Act of 1998. The Integrated Water Resources Management framework provides for collaborative strategic planning, shared visioning, consideration to water resource protection, attention to the regulation of use, operational planning, and implementation of management plans. Water users, with different stakes and views of how the resource should be managed, are expected to arrive at a single strategic plan for a specific hydrological region. Clearly this complex planning situation creates a need for tools that assist in producing a measure of convergence in thinking and enough of a shared rationale to allow stakeholder participation to produce an integrated management outcome. Several such tools are available in the overall catchment management strategy, but these would benefit from clearer understanding of the positions from which different stakeholders are operating and a way of knowing whether these positions are aligning. In this paper challenges posed by differences in meaning and understanding amongst stakeholders are examined against the need to engage stakeholders in water resources management. We deliberate on the prospects of employing mental model methodologies within the context of the strategic management framework for water management described.

A canopy trimming experiment in Puerto Rico: the response of litter invertebrate communities to canopy loss and debris deposition in a tropical forest subject to hurricanes

Science.gov (United States)

Barbara A. Richardson; Michael J. Richardson; Grizelle Gonzalez; Aaron B. Shiels; Diane S. Srivastava

2010-01-01

Hurricanes cause canopy removal and deposition of pulses of litter to the forest floor. A Canopy Trimming Experiment (CTE) was designed to decouple these two factors, and to investigate the separate abiotic and biotic consequences of hurricane-type damage and monitor recovery processes. As part of this experiment, effects on forest floor invertebrate communities were...

Advancement of the methodology for automated integration of external hazards into level 1 PSA modeling. Technical report; Weiterentwicklung der Methodik zur automatisierten Integration uebergreifender Einwirkungen in PSA-Modelle der Stufe 1. Technischer Fachbericht

Energy Technology Data Exchange (ETDEWEB)

Berner, Nadine; Herb, Joachim

2017-03-15

In the course of the research and development project RS1539 funded by the German Federal Ministry for Economics and Energy (BMWi) the methodology for the automated integration of hazards in Level 1 PSA models has been enhanced. Thereby, the analysis tool pyRiskRobot provides the methodological framework for mapping a generic spectrum of internal and external hazards onto complex PSA plant models. The reimplementation of the software tool via the programming language python extends the applicability and facilitates the handling of pyRiskRobot in comparison to the previous Ruby-based version RiskRobot. Moreover, the development of functions to perform the topological modelling of fault trees and the probabilistic specification of modified fault tree elements have been continued. Due to the reimplementation and further developments, the tool enables to systematically generate fault trees of varying complexity, to flexibly integrate fault trees in existing PSA models and to automatically duplicate interconnected topologies. Thus, pyRiskRobot allows the efficient and traceable realization of hazard specific, usually laborious modifications of PSA models. In addition, pyRiskRobot has been extended to serve as a functional interface between the data compilations comprising the potential influences of hazards on PSA relevant components and the data base of a PSA plant model. Based on this conceptual design, additional analyses of the data can be carried out prior to the integration within the PSA model topology. The reimplemented functionalities of pyRiskRobot have been validated with respect to reference applications, such as the modelling of an internal fire scenario, against the previous version RiskRobot. The existing method collection for the automated modification of fault tree topologies has been extended based on the requirements for further applications, among others the modelling of an external flooding scenario. The deduced hazard specific modelling approaches

Web survey methodology

CERN Document Server

Callegaro, Mario; Vehovar, Asja

2015-01-01

Web Survey Methodology guides the reader through the past fifteen years of research in web survey methodology. It both provides practical guidance on the latest techniques for collecting valid and reliable data and offers a comprehensive overview of research issues. Core topics from preparation to questionnaire design, recruitment testing to analysis and survey software are all covered in a systematic and insightful way. The reader will be exposed to key concepts and key findings in the literature, covering measurement, non-response, adjustments, paradata, and cost issues. The book also discusses the hottest research topics in survey research today, such as internet panels, virtual interviewing, mobile surveys and the integration with passive measurements, e-social sciences, mixed modes and business intelligence. The book is intended for students, practitioners, and researchers in fields such as survey and market research, psychological research, official statistics and customer satisfaction research.

Methodology and software to detect viral integration site hot-spots

Science.gov (United States)

2011-01-01

Background Modern gene therapy methods have limited control over where a therapeutic viral vector inserts into the host genome. Vector integration can activate local gene expression, which can cause cancer if the vector inserts near an oncogene. Viral integration hot-spots or 'common insertion sites' (CIS) are scrutinized to evaluate and predict patient safety. CIS are typically defined by a minimum density of insertions (such as 2-4 within a 30-100 kb region), which unfortunately depends on the total number of observed VIS. This is problematic for comparing hot-spot distributions across data sets and patients, where the VIS numbers may vary. Results We develop two new methods for defining hot-spots that are relatively independent of data set size. Both methods operate on distributions of VIS across consecutive 1 Mb 'bins' of the genome. The first method 'z-threshold' tallies the number of VIS per bin, converts these counts to z-scores, and applies a threshold to define high density bins. The second method 'BCP' applies a Bayesian change-point model to the z-scores to define hot-spots. The novel hot-spot methods are compared with a conventional CIS method using simulated data sets and data sets from five published human studies, including the X-linked ALD (adrenoleukodystrophy), CGD (chronic granulomatous disease) and SCID-X1 (X-linked severe combined immunodeficiency) trials. The BCP analysis of the human X-linked ALD data for two patients separately (774 and 1627 VIS) and combined (2401 VIS) resulted in 5-6 hot-spots covering 0.17-0.251% of the genome and containing 5.56-7.74% of the total VIS. In comparison, the CIS analysis resulted in 12-110 hot-spots covering 0.018-0.246% of the genome and containing 5.81-22.7% of the VIS, corresponding to a greater number of hot-spots as the data set size increased. Our hot-spot methods enable one to evaluate the extent of VIS clustering, and formally compare data sets in terms of hot-spot overlap. Finally, we show that the

A vision on methodology for integrated sustainable urban development: bequest

NARCIS (Netherlands)

Bentivegna, V.; Curwell, S.; Deakin, M.; Lombardi, P.; Mitchell, G.; Nijkamp, P.

2002-01-01

The concepts and visions of sustainable development that have emerged in the post-Brundtland era are explored in terms laying the foundations for a common vision of sustainable urban development (SUD). The described vision and methodology for SUD resulted from the activities of an international

Mechatronics methodology: 15 years of experience

Directory of Open Access Journals (Sweden)

Efren Gorrostieta

2015-09-01

Full Text Available This article presents a methodology to teach students to develop mechatronic projects. It was taught in higher education schools, in different universities in Mexico, in courses such as: Robotics, Control Systems, Mechatronic Systems, Artificial Intelligence, etc. The intention of this methodology is not only to achieve the integration of different subjects but also to accomplish synergy between them so that the final result may be the best possible in quality, time and robustness. Since its introduction into the educational area, this methodology was evaluated and modified for approximately five years, were substantial characteristics were adopted. For the next ten years, only minor alterations were carried out. Fifteen years of experience have proven that the methodology is useful not only for training but also for real projects. In this article, we first explain the methodology and its main characteristics, as well as a brief history of its teaching in different educational programs. Then, we present two cases were the methodology was successfully applied. The first project consisted in the design, construction and evaluation of a mobile robotic manipulator which aims to be used as an explosives ordnance device. In the second case, we document the results of a project assignment for robotics tasks carried out by students which were formerly taught with the methodology.

Quantifying biopsychosocial aspects in everyday contexts: an integrative methodological approach from the behavioral sciences

Science.gov (United States)

Portell, Mariona; Anguera, M Teresa; Hernández-Mendo, Antonio; Jonsson, Gudberg K

2015-01-01

Contextual factors are crucial for evaluative research in psychology, as they provide insights into what works, for whom, in what circumstances, in what respects, and why. Studying behavior in context, however, poses numerous methodological challenges. Although a comprehensive framework for classifying methods seeking to quantify biopsychosocial aspects in everyday contexts was recently proposed, this framework does not contemplate contributions from observational methodology. The aim of this paper is to justify and propose a more general framework that includes observational methodology approaches. Our analysis is rooted in two general concepts: ecological validity and methodological complementarity. We performed a narrative review of the literature on research methods and techniques for studying daily life and describe their shared properties and requirements (collection of data in real time, on repeated occasions, and in natural settings) and classification criteria (eg, variables of interest and level of participant involvement in the data collection process). We provide several examples that illustrate why, despite their higher costs, studies of behavior and experience in everyday contexts offer insights that complement findings provided by other methodological approaches. We urge that observational methodology be included in classifications of research methods and techniques for studying everyday behavior and advocate a renewed commitment to prioritizing ecological validity in behavioral research seeking to quantify biopsychosocial aspects. PMID:26089708

Enviro-HIRLAM online integrated meteorology–chemistry modelling system: strategy, methodology, developments and applications (v7.2

Directory of Open Access Journals (Sweden)

A. Baklanov

2017-08-01

Full Text Available The Environment – High Resolution Limited Area Model (Enviro-HIRLAM is developed as a fully online integrated numerical weather prediction (NWP and atmospheric chemical transport (ACT model for research and forecasting of joint meteorological, chemical and biological weather. The integrated modelling system is developed by the Danish Meteorological Institute (DMI in collaboration with several European universities. It is the baseline system in the HIRLAM Chemical Branch and used in several countries and different applications. The development was initiated at DMI more than 15 years ago. The model is based on the HIRLAM NWP model with online integrated pollutant transport and dispersion, chemistry, aerosol dynamics, deposition and atmospheric composition feedbacks. To make the model suitable for chemical weather forecasting in urban areas, the meteorological part was improved by implementation of urban parameterisations. The dynamical core was improved by implementing a locally mass-conserving semi-Lagrangian numerical advection scheme, which improves forecast accuracy and model performance. The current version (7.2, in comparison with previous versions, has a more advanced and cost-efficient chemistry, aerosol multi-compound approach, aerosol feedbacks (direct and semi-direct on radiation and (first and second indirect effects on cloud microphysics. Since 2004, the Enviro-HIRLAM has been used for different studies, including operational pollen forecasting for Denmark since 2009 and operational forecasting atmospheric composition with downscaling for China since 2017. Following the main research and development strategy, further model developments will be extended towards the new NWP platform – HARMONIE. Different aspects of online coupling methodology, research strategy and possible applications of the modelling system, and fit-for-purpose model configurations for the meteorological and air quality communities are discussed.

The Integrated Renovation Process

DEFF Research Database (Denmark)

Galiotto, Nicolas; Heiselberg, Per; Knudstrup, Mary-Ann

The Integrated Renovation Process (IRP) is a user customized methodology based on judiciously selected constructivist and interactive multi-criteria decision making methods (Galiotto, Heiselberg, & Knudstrup, 2014 (expected)). When applied for home renovation, the Integrated Renovation Process...

Human-Systems Integration (HSI) Methodology Development for NASA

Data.gov (United States)

National Aeronautics and Space Administration — Human-Systems Integration (HSI) refers to design activities associated with ensuring that manpower, personnel, training, human factors engineering, safety, health...

Assessment of Wind Turbine Structural Integrity using Response Surface Methodology

DEFF Research Database (Denmark)

Toft, Henrik Stensgaard; Svenningsen, Lasse; Moser, Wolfgang

2016-01-01

Highlights •A new approach to assessment of site specific wind turbine loads is proposed. •The approach can be applied in both fatigue and ultimate limit state. •Two different response surface methodologies have been investigated. •The model uncertainty introduced by the response surfaces...

Risk-based methodology for USNRC inspections

International Nuclear Information System (INIS)

Wong, S.M.; Holahan, G.M.; Chung, J.W.; Johnson, M.R.

1995-01-01

This paper describes the development and trial applications of a risk-based methodology to enhance the inspection processes for US nuclear power plants. Objectives of risk-based methods to complement prescriptive engineering approaches in US Nuclear Regulatory Commission (USNRC) inspection programs are presented. Insights from time-dependent risk profiles of plant configurational from Individual Plant Evaluation (IPE) studies were integrated to develop a framework for optimizing inspection efforts in NRC regulatory initiatives. Lessons learned from NRC pilot applications of the risk-based methodology for evaluation of the effectiveness of operational risk management programs at US nuclear power plant sites are also discussed

CATHARE code development and assessment methodologies

International Nuclear Information System (INIS)

Micaelli, J.C.; Barre, F.; Bestion, D.

1995-01-01

The CATHARE thermal-hydraulic code has been developed jointly by Commissariat a l'Energie Atomique (CEA), Electricite de France (EdF), and Framatorne for safety analysis. Since the beginning of the project (September 1979), development and assessment activities have followed a methodology supported by two series of experimental tests: separate effects tests and integral effects tests. The purpose of this paper is to describe this methodology, the code assessment status, and the evolution to take into account two new components of this program: the modeling of three-dimensional phenomena and the requirements of code uncertainty evaluation

Mo(ve)ment-methodology

DEFF Research Database (Denmark)

Mørck, Line Lerche; Christian Celosse-Andersen, Martin

2018-01-01

This paper describes the theoretical basis for and development of a moment-movement research methodology, based on an integration of critical psychological practice research and critical ethnographic social practice theory. Central theoretical conceptualizations, such as human agency, life...... conditions and identity formation, are discussed in relation to criminological theories of gang desistance. The paper illustrates how the mo(ve)ment methodology was applied in a study of comprehensive processes of identity (re)formation and gang exit processes. This study was conducted with Martin, a former....... This is a moment which captures Martin’s complex and ambiguous feelings of conflictual concerns, frustration, anger, and a new feeling of insecurity in his masculinity, as well as engagement and a sense of deep meaningfulness as he becomes a more reflective academic. All these conflicting feelings also give...

Safety and reliability of pressure components with special emphasis on the contribution of component and large specimen testing to structural integrity assessment methodology. Vol. 1 and 2

International Nuclear Information System (INIS)

1987-01-01

The 51 papers of the 13. MPA-seminar contribute to structural integrity assessment methodology with special emphasis on the component and large specimen testing. 8 of the papers deal with fracture mechanics, 6 papers with dynamic loading, 13 papers with nondestructive testing, 2 papers with radiation embrittlement, 5 papers with pipe failure, 4 papers with components, 2 papers with thermal shock loading, 5 papers with the high temperature behaviour, 4 papers with the integrity of vessels and 3 papers with the integrity of welded joints. Especially also the fracture behaviour of steel material is verificated. All papers are separately indexed and analysed for the database. (DG) [de

A Systems Engineering Methodology for Designing and Planning the Built Environment—Results from the Urban Research Laboratory Nuremberg and Their Integration in Education

Directory of Open Access Journals (Sweden)

Philipp Geyer

2014-04-01

Full Text Available Sustainable urban development requires a long-term sector-integrative approach. This paper proposes a method of system analysis and partial simulation for urban structures for this purpose. It couples a discussion-based holistic approach for systems analysis and modelling of urban structures with quantitative modelling and simulation of partial scenarios that serve to examine specific questions regarding the long-term development of urban structures. In the first part, the application in the City Lab Nuremberg West, a multidisciplinary urban research laboratory, serves to develop the methodology and its illustration. The main objective is to examine the transition of the existing underperforming quarter to a sustainable and livable urban environment. Scenario-based experiments with respect to development paths determine robustness and risks of different configurations. The second part of the paper describes the transfer of the methodology to education. The approach serves to teach students in the Energy-Efficient and Sustainable Building master course program an integrative way of planning a sustainable built environment. The definition of educational objectives concerning the students’ understanding and management of systemic interdependencies of sustainability help assess the use of the method in the classroom. The aim is to provide them with the competence to develop strategies for complex situations while planning a sustainable built environment.

Integrated Deterministic-Probabilistic Safety Assessment Methodologies

Energy Technology Data Exchange (ETDEWEB)

Kudinov, P.; Vorobyev, Y.; Sanchez-Perea, M.; Queral, C.; Jimenez Varas, G.; Rebollo, M. J.; Mena, L.; Gomez-Magin, J.

2014-02-01

IDPSA (Integrated Deterministic-Probabilistic Safety Assessment) is a family of methods which use tightly coupled probabilistic and deterministic approaches to address respective sources of uncertainties, enabling Risk informed decision making in a consistent manner. The starting point of the IDPSA framework is that safety justification must be based on the coupling of deterministic (consequences) and probabilistic (frequency) considerations to address the mutual interactions between stochastic disturbances (e.g. failures of the equipment, human actions, stochastic physical phenomena) and deterministic response of the plant (i.e. transients). This paper gives a general overview of some IDPSA methods as well as some possible applications to PWR safety analyses. (Author)

Criticality accident studies and methodology implemented at the CEA

International Nuclear Information System (INIS)

Barbry, Francis; Fouillaud, Patrick; Reverdy, Ludovic; Mijuin, Dominique

2003-01-01

Based on the studies and results of experimental programs performed since 1967 in the CRAC, then SILENE facilities, the CEA has devised a methodology for criticality accident studies. This methodology integrates all the main focuses of its approach, from criticality accident phenomenology to emergency planning and response, and thus includes aspects such as criticality alarm detector triggering, airborne releases, and irradiation risk assessment. (author)

An integrated methodology on the suitability of offshore sites for wind farm development

Science.gov (United States)

Patlakas, Platon; Galanis, George; Péray, Marie; Filipot, Jean-François; Kalogeri, Christina; Spyrou, Christos; Diamantis, Dimitris; Kallos, Gerorge

2016-04-01

During, the last decades the potential and interest in wind energy investments has been constantly increasing in the European countries. As technology changes rapidly, more and more areas can be identified as suitable for energy applications. Offshore wind farms perfectly illustrate how new technologies allow to build bigger, more efficient and resistant in extreme conditions wind power plants. The current work proposes an integrated methodology to determine the suitability of an offshore marine area for the development of wind farm structures. More specifically, the region of interest is evaluated based both on the natural resources, connected to the local environmental characteristics, and potential constrains set by anthropogenic or other activities. State of the art atmospheric and wave models and a 10-year hindcast database are utilized in conjunction with local information for a number of potential constrains, leading to a 5-scale suitability index for the whole area. In this way, sub regions are characterized, at a high resolution mode, as poorly or highly suitable for wind farm development, providing a new tool for technical/research teams and decision makers. In addition, extreme wind and wave conditions and their 50-years return period are analyzed and used to define the safety level of the wind farms structural characteristics.

Proteomic profiling of human keratinocytes undergoing UVB-induced alternative differentiation reveals TRIpartite Motif Protein 29 as a survival factor.

Directory of Open Access Journals (Sweden)

Véronique Bertrand-Vallery

Full Text Available BACKGROUND: Repeated exposures to UVB of human keratinocytes lacking functional p16(INK-4a and able to differentiate induce an alternative state of differentiation rather than stress-induced premature senescence. METHODOLOGY/PRINCIPAL FINDINGS: A 2D-DIGE proteomic profiling of this alternative state of differentiation was performed herein at various times after the exposures to UVB. Sixty-nine differentially abundant protein species were identified by mass spectrometry, many of which are involved in keratinocyte differentiation and survival. Among these protein species was TRIpartite Motif Protein 29 (TRIM29. Increased abundance of TRIM29 following UVB exposures was validated by Western blot using specific antibody and was also further analysed by immunochemistry and by RT-PCR. TRIM29 was found very abundant in keratinocytes and reconstructed epidermis. Knocking down the expression of TRIM29 by short-hairpin RNA interference decreased the viability of keratinocytes after UVB exposure. The abundance of involucrin mRNA, a marker of late differentiation, increased concomitantly. In TRIM29-knocked down reconstructed epidermis, the presence of picnotic cells revealed cell injury. Increased abundance of TRIM29 was also observed upon exposure to DNA damaging agents and PKC activation. The UVB-induced increase of TRIM29 abundance was dependent on a PKC signaling pathway, likely PKCdelta. CONCLUSIONS/SIGNIFICANCE: These findings suggest that TRIM29 allows keratinocytes to enter a protective alternative differentiation process rather than die massively after stress.

A Generic Methodology for Superstructure Optimization of Different Processing Networks

DEFF Research Database (Denmark)

Bertran, Maria-Ona; Frauzem, Rebecca; Zhang, Lei

2016-01-01

In this paper, we propose a generic computer-aided methodology for synthesis of different processing networks using superstructure optimization. The methodology can handle different network optimization problems of various application fields. It integrates databases with a common data architecture......, a generic model to represent the processing steps, and appropriate optimization tools. A special software interface has been created to automate the steps in the methodology workflow, allow the transfer of data between tools and obtain the mathematical representation of the problem as required...

METHODOLOGY OF PROFESSIONAL PEDAGOGICAL EDUCATION: THEORY AND PRACTICE (theoretical and methodological foundations of vocational teacher education

Directory of Open Access Journals (Sweden)

Evgeny M. Dorozhkin

2014-01-01

Full Text Available The study is aimed at investigating a justification of the new approach to the problem of vocational education development through the prism of interdependence research methodology and practice. This conceptual setup allows determining the main directions for teacher training modernization of vocational schools. The authors note that the current socio-economic situation in our country has actualized the problem of personnel training. Politicians, economists and scientists’ speeches are all about the shortage of skilled personnel. They see the main reason of this catastrophic situation in the present system of primary and secondary vocational education. At least they concern over the current practice of pedagogical personnel training of vocational education who are to restore the system of vocational education. Our country, Russia has a great positive experience in solving this problem. Scientific-methodological centre for vocational teacher education is the Russian State Vocational Pedagogical University under the scientific direction of Academician of the Russian Academy of Education, G. M. Romantsev. The reflection of scientifictheoretical bases of this education led the authors to the analysis and designing (formation of existent and new professional and pedagogical methodology. Methods. The fundamental position of A. M. Novikov on the generality of the research (scientific and practical activity methodology has become the theoretical platform of the present study. Conceptual field, conceptual statements and professional model are presented as the whole system (or integrating factor. The theoretical framework has determined the logic of the study and its results. Scientific and educational methodology differentiation in terms of the subject of cognitive activity has allowed identifying the main scientific and practical disciplines of vocational teacher education. The creative concept as the subject ground is instrumental analysis of

Integrated Control Using the SOFFT Control Structure

Science.gov (United States)

Halyo, Nesim

1996-01-01

The need for integrated/constrained control systems has become clearer as advanced aircraft introduced new coupled subsystems such as new propulsion subsystems with thrust vectoring and new aerodynamic designs. In this study, we develop an integrated control design methodology which accomodates constraints among subsystem variables while using the Stochastic Optimal Feedforward/Feedback Control Technique (SOFFT) thus maintaining all the advantages of the SOFFT approach. The Integrated SOFFT Control methodology uses a centralized feedforward control and a constrained feedback control law. The control thus takes advantage of the known coupling among the subsystems while maintaining the identity of subsystems for validation purposes and the simplicity of the feedback law to understand the system response in complicated nonlinear scenarios. The Variable-Gain Output Feedback Control methodology (including constant gain output feedback) is extended to accommodate equality constraints. A gain computation algorithm is developed. The designer can set the cross-gains between two variables or subsystems to zero or another value and optimize the remaining gains subject to the constraint. An integrated control law is designed for a modified F-15 SMTD aircraft model with coupled airframe and propulsion subsystems using the Integrated SOFFT Control methodology to produce a set of desired flying qualities.

Excess electricity diagrams and the integration of renewable energy

DEFF Research Database (Denmark)

Lund, Henrik

2004-01-01

The article presents a methodology of showing the rate of integration off specific renewable energy sources into the electricity supply system.......The article presents a methodology of showing the rate of integration off specific renewable energy sources into the electricity supply system....

Disruption of the ASTN2/TRIM32 locus at 9q33.1 is a risk factor in males for autism spectrum disorders, ADHD and other neurodevelopmental phenotypes

Science.gov (United States)

Lionel, Anath C.; Tammimies, Kristiina; Vaags, Andrea K.; Rosenfeld, Jill A.; Ahn, Joo Wook; Merico, Daniele; Noor, Abdul; Runke, Cassandra K.; Pillalamarri, Vamsee K.; Carter, Melissa T.; Gazzellone, Matthew J.; Thiruvahindrapuram, Bhooma; Fagerberg, Christina; Laulund, Lone W.; Pellecchia, Giovanna; Lamoureux, Sylvia; Deshpande, Charu; Clayton-Smith, Jill; White, Ann C.; Leather, Susan; Trounce, John; Melanie Bedford, H.; Hatchwell, Eli; Eis, Peggy S.; Yuen, Ryan K.C.; Walker, Susan; Uddin, Mohammed; Geraghty, Michael T.; Nikkel, Sarah M.; Tomiak, Eva M.; Fernandez, Bridget A.; Soreni, Noam; Crosbie, Jennifer; Arnold, Paul D.; Schachar, Russell J.; Roberts, Wendy; Paterson, Andrew D.; So, Joyce; Szatmari, Peter; Chrysler, Christina; Woodbury-Smith, Marc; Brian Lowry, R.; Zwaigenbaum, Lonnie; Mandyam, Divya; Wei, John; MacDonald, Jeffrey R.; Howe, Jennifer L.; Nalpathamkalam, Thomas; Wang, Zhuozhi; Tolson, Daniel; Cobb, David S.; Wilks, Timothy M.; Sorensen, Mark J.; Bader, Patricia I.; An, Yu; Wu, Bai-Lin; Musumeci, Sebastiano Antonino; Romano, Corrado; Postorivo, Diana; Nardone, Anna M.; Monica, Matteo Della; Scarano, Gioacchino; Zoccante, Leonardo; Novara, Francesca; Zuffardi, Orsetta; Ciccone, Roberto; Antona, Vincenzo; Carella, Massimo; Zelante, Leopoldo; Cavalli, Pietro; Poggiani, Carlo; Cavallari, Ugo; Argiropoulos, Bob; Chernos, Judy; Brasch-Andersen, Charlotte; Speevak, Marsha; Fichera, Marco; Ogilvie, Caroline Mackie; Shen, Yiping; Hodge, Jennelle C.; Talkowski, Michael E.; Stavropoulos, Dimitri J.; Marshall, Christian R.; Scherer, Stephen W.

2014-01-01

Rare copy number variants (CNVs) disrupting ASTN2 or both ASTN2 and TRIM32 have been reported at 9q33.1 by genome-wide studies in a few individuals with neurodevelopmental disorders (NDDs). The vertebrate-specific astrotactins, ASTN2 and its paralog ASTN1, have key roles in glial-guided neuronal migration during brain development. To determine the prevalence of astrotactin mutations and delineate their associated phenotypic spectrum, we screened ASTN2/TRIM32 and ASTN1 (1q25.2) for exonic CNVs in clinical microarray data from 89 985 individuals across 10 sites, including 64 114 NDD subjects. In this clinical dataset, we identified 46 deletions and 12 duplications affecting ASTN2. Deletions of ASTN1 were much rarer. Deletions near the 3′ terminus of ASTN2, which would disrupt all transcript isoforms (a subset of these deletions also included TRIM32), were significantly enriched in the NDD subjects (P = 0.002) compared with 44 085 population-based controls. Frequent phenotypes observed in individuals with such deletions include autism spectrum disorder (ASD), attention deficit hyperactivity disorder (ADHD), speech delay, anxiety and obsessive compulsive disorder (OCD). The 3′-terminal ASTN2 deletions were significantly enriched compared with controls in males with NDDs, but not in females. Upon quantifying ASTN2 human brain RNA, we observed shorter isoforms expressed from an alternative transcription start site of recent evolutionary origin near the 3′ end. Spatiotemporal expression profiling in the human brain revealed consistently high ASTN1 expression while ASTN2 expression peaked in the early embryonic neocortex and postnatal cerebellar cortex. Our findings shed new light on the role of the astrotactins in psychopathology and their interplay in human neurodevelopment. PMID:24381304

Flight Investigation to Determine the Effect of Jet Exhaust on Drag, Trim Characteristics, and Afterbody Pressures of a 0.125-Scale Rocket Model of the Mcdonnell F-101A Airplane

Science.gov (United States)

Kennedy, Thomas L.

1956-01-01

A flight investigation was conducted to determine the effect of jet exhaust on the drag, trim characteristics, and afterbody pressures on a 0.125-scale rocket model of the McDonnell F-101A airplance. Power-off data were obtained over a Mach number range of 1.04 to 1.9 and power-on data were obtained at a Mach number of about 1.5. The data indicated that with power-on the change in external drag coefficient was within the data accuracy and there was a decrease in trim angle of attack of 1.27 degrees with a corresponding decrease of 0.07 in lift coefficient. Correspondingly, pressure coefficients on the side and bottom of the fuselage indicated a positive increment near the jet exit. As the distance downstream of the jet exit increased, the increment on the bottom of the fuselage increased, whereas the increments on the side decreased to a negative peak.

Hanford Site Risk Assessment Methodology. Revision 3

International Nuclear Information System (INIS)

1995-05-01

This methodology has been developed to prepare human health and ecological evaluations of risk as part of the Comprehensive Environmental Response, Compensation, and Liability Act of 1980 (CERCLA) remedial investigations (RI) and the Resource conservation and Recovery Act of 1976 (RCRA) facility investigations (FI) performed at the Hanford Site pursuant to the hanford Federal Facility Agreement and Consent Order (Ecology et al. 1994), referred to as the Tri-Party Agreement. Development of the methodology has been undertaken so that Hanford Site risk assessments are consistent with current regulations and guidance, while providing direction on flexible, ambiguous, or undefined aspects of the guidance. The methodology identifies site-specific risk assessment considerations and integrates them with approaches for evaluating human and ecological risk that can be factored into the risk assessment program supporting the Hanford Site cleanup mission. Consequently, the methodology will enhance the preparation and review of individual risk assessments at the Hanford Site

Forming professional mobility of a future teacher: methodological approaches

Directory of Open Access Journals (Sweden)

Prima Raisa Nikolaevna

2011-10-01

Full Text Available Analyzes the theoretical and methodological principles of occupational mobility of future teachers. Focuses on the motivation of the choice of systems methodology, the "system integrity" as the dominant conceptual study. It is noted that the educational process of higher education provides an opportunity: the implementation of integration and differentiation of learning content, technology, formation of professional mobility, the stimulating activity of quasi professional future teachers, mainstream interdisciplinary connections. It is established that the formation of a mobile professional organization provides some of the "field capacity", education and upbringing environment, life. It focuses on the future of professional diversity of choice of the status of growth, professional development, rapid orientation (adapted, actively striving to change the unfavorable situation.

Alternative Procedure of Heat Integration Tehnique Election between Two Unit Processes to Improve Energy Saving

Science.gov (United States)

Santi, S. S.; Renanto; Altway, A.

2018-01-01

The energy use system in a production process, in this case heat exchangers networks (HENs), is one element that plays a role in the smoothness and sustainability of the industry itself. Optimizing Heat Exchanger Networks (HENs) from process streams can have a major effect on the economic value of an industry as a whole. So the solving of design problems with heat integration becomes an important requirement. In a plant, heat integration can be carried out internally or in combination between process units. However, steps in the determination of suitable heat integration techniques require long calculations and require a long time. In this paper, we propose an alternative step in determining heat integration technique by investigating 6 hypothetical units using Pinch Analysis approach with objective function energy target and total annual cost target. The six hypothetical units consist of units A, B, C, D, E, and F, where each unit has the location of different process streams to the temperature pinch. The result is a potential heat integration (ΔH’) formula that can trim conventional steps from 7 steps to just 3 steps. While the determination of the preferred heat integration technique is to calculate the potential of heat integration (ΔH’) between the hypothetical process units. Completion of calculation using matlab language programming.

Hanford Site baseline risk assessment methodology. Revision 2

Energy Technology Data Exchange (ETDEWEB)

1993-03-01

This methodology has been developed to prepare human health and environmental evaluations of risk as part of the Comprehensive Environmental Response, Compensation, and Liability Act remedial investigations (RIs) and the Resource Conservation and Recovery Act facility investigations (FIs) performed at the Hanford Site pursuant to the Hanford Federal Facility Agreement and Consent Order referred to as the Tri-Party Agreement. Development of the methodology has been undertaken so that Hanford Site risk assessments are consistent with current regulations and guidance, while providing direction on flexible, ambiguous, or undefined aspects of the guidance. The methodology identifies Site-specific risk assessment considerations and integrates them with approaches for evaluating human and environmental risk that can be factored into the risk assessment program supporting the Hanford Site cleanup mission. Consequently, the methodology will enhance the preparation and review of individual risk assessments at the Hanford Site.

SafeNet: a methodology for integrating general-purpose unsafe devices in safe-robot rehabilitation systems.

Science.gov (United States)

Vicentini, Federico; Pedrocchi, Nicola; Malosio, Matteo; Molinari Tosatti, Lorenzo

2014-09-01

Robot-assisted neurorehabilitation often involves networked systems of sensors ("sensory rooms") and powerful devices in physical interaction with weak users. Safety is unquestionably a primary concern. Some lightweight robot platforms and devices designed on purpose include safety properties using redundant sensors or intrinsic safety design (e.g. compliance and backdrivability, limited exchange of energy). Nonetheless, the entire "sensory room" shall be required to be fail-safe and safely monitored as a system at large. Yet, sensor capabilities and control algorithms used in functional therapies require, in general, frequent updates or re-configurations, making a safety-grade release of such devices hardly sustainable in cost-effectiveness and development time. As such, promising integrated platforms for human-in-the-loop therapies could not find clinical application and manufacturing support because of lacking in the maintenance of global fail-safe properties. Under the general context of cross-machinery safety standards, the paper presents a methodology called SafeNet for helping in extending the safety rate of Human Robot Interaction (HRI) systems using unsafe components, including sensors and controllers. SafeNet considers, in fact, the robotic system as a device at large and applies the principles of functional safety (as in ISO 13489-1) through a set of architectural procedures and implementation rules. The enabled capability of monitoring a network of unsafe devices through redundant computational nodes, allows the usage of any custom sensors and algorithms, usually planned and assembled at therapy planning-time rather than at platform design-time. A case study is presented with an actual implementation of the proposed methodology. A specific architectural solution is applied to an example of robot-assisted upper-limb rehabilitation with online motion tracking. Copyright © 2014 Elsevier Ireland Ltd. All rights reserved.

Advantages of Westinghouse BWR control rod drop accidents methodology utilizing integrated POLCA-T code

International Nuclear Information System (INIS)

Panayotov, Dobromir

2008-01-01

The paper focuses on the activities pursued by Westinghouse in the development and licensing of POLCA-T code Control Rod Drop Accident (CRDA) Methodology. The comprehensive CRDA methodology that utilizes PHOENIX4/POLCA7/POLCA-T calculation chain foresees complete cycle-specific analysis. The methodology consists of determination of candidates of control rods (CR) that could cause a significant reactivity excursion if dropped throughout the entire fuel cycle, selection of limiting initial conditions for CRDA transient simulation and transient simulation itself. The Westinghouse methodology utilizes state-of-the-art methods. Unnecessary conservatisms in the methodology have been avoided to allow the accurate prediction of margin to design bases. This is mainly achieved by using the POLCA-T code for dynamic CRDA evaluations. The code belongs to the same calculation chain that is used for core design. Thus the very same reactor, core, cycle and fuel data base is used. This allows also reducing the uncertainties of input data and parameters that determine the energy deposition in the fuel. Uncertainty treatment, very selective use of conservatisms, selection of the initial conditions for limiting case analyses, incorporation into POLCA-T code models of the licensed fuel performance code are also among the means of performing realistic CRDA transient analyses. (author)

Understanding palliative care on the heart failure care team: an innovative research methodology.

Science.gov (United States)

Lingard, Lorelei A; McDougall, Allan; Schulz, Valerie; Shadd, Joshua; Marshall, Denise; Strachan, Patricia H; Tait, Glendon R; Arnold, J Malcolm; Kimel, Gil

2013-05-01

There is a growing call to integrate palliative care for patients with advanced heart failure (HF). However, the knowledge to inform integration efforts comes largely from interview and survey research with individual patients and providers. This work has been critically important in raising awareness of the need for integration, but it is insufficient to inform solutions that must be enacted not by isolated individuals but by complex care teams. Research methods are urgently required to support systematic exploration of the experiences of patients with HF, family caregivers, and health care providers as they interact as a care team. To design a research methodology that can support systematic exploration of the experiences of patients with HF, caregivers, and health care providers as they interact as a care team. This article describes in detail a methodology that we have piloted and are currently using in a multisite study of HF care teams. We describe three aspects of the methodology: the theoretical framework, an innovative sampling strategy, and an iterative system of data collection and analysis that incorporates four data sources and four analytical steps. We anticipate that this innovative methodology will support groundbreaking research in both HF care and other team settings in which palliative integration efforts are emerging for patients with advanced nonmalignant disease. Copyright © 2013 U.S. Cancer Pain Relief Committee. Published by Elsevier Inc. All rights reserved.

Marrying Step Feed with Secondary Clarifier Improvements to Significantly Increase Peak Wet Weather Treatment Capacity: An Integrated Methodology.

Science.gov (United States)

Daigger, Glen T; Siczka, John S; Smith, Thomas F; Frank, David A; McCorquodale, J A

2017-08-01

  The need to increase the peak wet weather secondary treatment capacity of the City of Akron, Ohio, Water Reclamation Facility (WRF) provided the opportunity to test an integrated methodology for maximizing the peak wet weather secondary treatment capacity of activated sludge systems. An initial investigation, consisting of process modeling of the secondary treatment system and computational fluid dynamics (CFD) analysis of the existing relatively shallow secondary clarifiers (3.3 and 3.7 m sidewater depth in 30.5 m diameter units), indicated that a significant increase in capacity from 416 000 to 684 000 m3/d or more was possible by adding step feed capabilities to the existing bioreactors and upgrading the existing secondary clarifiers. One of the six treatment units at the WRF was modified, and an extensive 2-year testing program was conducted to determine the total peak wet weather secondary treatment capacity achievable. The results demonstrated that a peak wet weather secondary treatment capacity approaching 974 000 m3/d is possible as long as secondary clarifier solids and hydraulic loadings could be separately controlled using the step feed capability provided. Excellent sludge settling characteristics are routinely experienced at the City of Akron WRF, raising concerns that the identified peak wet weather secondary treatment capacity could not be maintained should sludge settling characteristics deteriorate for some reason. Computational fluid dynamics analysis indicated that the impact of the deterioration of sludge settling characteristics could be mitigated and the identified peak wet weather secondary treatment capacity maintained by further use of the step feed capability provided to further reduce secondary clarifier solids loading rates at the identified high surface overflow rates. The results also demonstrated that effluent limits not only for total suspended solids (TSS) and five-day carbonaceous biochemical oxygen demand (cBOD5) could be

Priority research directions in the area of qualitative methodology

OpenAIRE

Melnikova, Olga; Khoroshilov, Dmitry

2010-01-01

The basic directions of modern theoretical and practical research in the area of qualitative methodology in Russia are discussed in the article. The complexity of research is considered from three points of view: the development of methodology of qualitative analysis, qualitative methods, and verbal and nonverbal projective techniques. The authors present an integrative model of the qualitative analysis, the research on specificity of the use of discourse-analysis method and projective techni...

The Societal Nature of Subjectivity: An Interdisciplinary Methodological Challenge

Directory of Open Access Journals (Sweden)

Henning Salling Olesen

2012-09-01

Full Text Available The thematic issue presents a psycho-societal approach to qualitative empirical research in several areas of everyday social life. It is an approach which integrates a theory of subjectivity and an interpretation methodology which integrates hermeneutic experiences from text analysis and psychoanalysis. Its particular focus is on subjectivity—as an aspect of the research object and as an aspect of the research process. By the term "approach" is indicated the intrinsic connection between the theorizing of an empirical object and the reflection of the research process and the epistemic subject. In terms of methodology it revives the themes originally launched in FQS exactly ten years ago: "Subjectivity and Reflectivity in Qualitative Research" (BREUER, MRUCK & ROTH, 2002; MRUCK & BREUER, 2003. This editorial introduction presents the intellectual background of the psycho-societal methodology, reflects on its relevance and critical perspectives in a contemporary landscape of social science, and comments the way in which an international and interdisciplinary research group has developed this approach to profane empirical research. URN: http://nbn-resolving.de/urn:nbn:de:0114-fqs120345

Evaluation of the threshold trimming method for micro inertial fluidic switch based on electrowetting technology

Directory of Open Access Journals (Sweden)

Tingting Liu

2014-03-01

Full Text Available The switch based on electrowetting technology has the advantages of no moving part, low contact resistance, long life and adjustable acceleration threshold. The acceleration threshold of switch can be fine-tuned by adjusting the applied voltage. This paper is focused on the electrowetting properties of switch and the influence of microchannel structural parameters, applied voltage and droplet volume on acceleration threshold. In the presence of process errors of micro inertial fluidic switch and measuring errors of droplet volume, there is a deviation between test acceleration threshold and target acceleration threshold. Considering the process errors and measuring errors, worst-case analysis is used to analyze the influence of parameter tolerance on the acceleration threshold. Under worst-case condition the total acceleration threshold tolerance caused by various errors is 9.95%. The target acceleration threshold can be achieved by fine-tuning the applied voltage. The acceleration threshold trimming method of micro inertial fluidic switch is verified.

New methodology to investigate potential contaminant mass fluxes at the stream-aquifer interface by combining integral pumping tests and streambed temperatures

International Nuclear Information System (INIS)

Kalbus, E.; Schmidt, C.; Bayer-Raich, M.; Leschik, S.; Reinstorf, F.; Balcke, G.U.; Schirmer, M.

2007-01-01

The spatial pattern and magnitude of mass fluxes at the stream-aquifer interface have important implications for the fate and transport of contaminants in river basins. Integral pumping tests were performed to quantify average concentrations of chlorinated benzenes in an unconfined aquifer partially penetrated by a stream. Four pumping wells were operated simultaneously for a time period of 5 days and sampled for contaminant concentrations. Streambed temperatures were mapped at multiple depths along a 60 m long stream reach to identify the spatial patterns of groundwater discharge and to quantify water fluxes at the stream-aquifer interface. The combined interpretation of the results showed average potential contaminant mass fluxes from the aquifer to the stream of 272 μg m -2 d -1 MCB and 71 μg m -2 d -1 DCB, respectively. This methodology combines a large-scale assessment of aquifer contamination with a high-resolution survey of groundwater discharge zones to estimate contaminant mass fluxes between aquifer and stream. - We provide a new methodology to quantify the potential contaminant mass flux from an aquifer to a stream

Proposal of Integrated Safety Assessment Methodology for Embedded System

International Nuclear Information System (INIS)

Sun, Wei; Kageyama, Makoto; Kanemoto, Shigeru

2011-01-01

To do risk analysis and risk evaluation for complicated safety critical embedded systems, there are three things should be paid a good attention: 1) an efficient and integrated model expression of embedded systems: 2) systematic risk analysis based on integrated system model: 3) quantitative risk evaluation for software and hardware integrated system. In this paper, taken electric water boiler as a target system, a proposal of risk analysis and risk evaluation for the embedded system is presented to meet these three purposes. In risk analysis, MFM is used and FT is generated automatically from MFM following some rules: And in risk evaluation, GO-FLOW is used to evaluate the reliability of sensors. And furthermore, FIT is applied to evaluate the safety software logic based on the diversity design concept. Although the electric water boiler is a simple example, it includes the key components of the embedded system like sensors, actuators, and software component. So, the process of modeling, analysis, and evaluation could be applied to other kinds of complicated embedded systems

Methodological integrative review of the work sampling technique used in nursing workload research.

Science.gov (United States)

Blay, Nicole; Duffield, Christine M; Gallagher, Robyn; Roche, Michael

2014-11-01

To critically review the work sampling technique used in nursing workload research. Work sampling is a technique frequently used by researchers and managers to explore and measure nursing activities. However, work sampling methods used are diverse making comparisons of results between studies difficult. Methodological integrative review. Four electronic databases were systematically searched for peer-reviewed articles published between 2002-2012. Manual scanning of reference lists and Rich Site Summary feeds from contemporary nursing journals were other sources of data. Articles published in the English language between 2002-2012 reporting on research which used work sampling to examine nursing workload. Eighteen articles were reviewed. The review identified that the work sampling technique lacks a standardized approach, which may have an impact on the sharing or comparison of results. Specific areas needing a shared understanding included the training of observers and subjects who self-report, standardization of the techniques used to assess observer inter-rater reliability, sampling methods and reporting of outcomes. Work sampling is a technique that can be used to explore the many facets of nursing work. Standardized reporting measures would enable greater comparison between studies and contribute to knowledge more effectively. Author suggestions for the reporting of results may act as guidelines for researchers considering work sampling as a research method. © 2014 John Wiley & Sons Ltd.

RAMA Methodology for the Calculation of Neutron Fluence

International Nuclear Information System (INIS)

Villescas, G.; Corchon, F.

2013-01-01

he neutron fluence plays an important role in the study of the structural integrity of the reactor vessel after a certain time of neutron irradiation. The NRC defined in the Regulatory Guide 1.190, the way must be estimated neutron fluence, including uncertainty analysis of the validation process (creep uncertainty is ? 20%). TRANSWARE Enterprises Inc. developed a methodology for calculating the neutron flux, 1,190 based guide, known as RAMA. Uncertainty values obtained with this methodology, for about 18 vessels, are less than 10%.

Scoring-and-unfolding trimmed tree assembler: concepts, constructs and comparisons.

Science.gov (United States)

Narzisi, Giuseppe; Mishra, Bud

2011-01-15

Mired by its connection to a well-known -complete combinatorial optimization problem-namely, the Shortest Common Superstring Problem (SCSP)-historically, the whole-genome sequence assembly (WGSA) problem has been assumed to be amenable only to greedy and heuristic methods. By placing efficiency as their first priority, these methods opted to rely only on local searches, and are thus inherently approximate, ambiguous or error prone, especially, for genomes with complex structures. Furthermore, since choice of the best heuristics depended critically on the properties of (e.g. errors in) the input data and the available long range information, these approaches hindered designing an error free WGSA pipeline. We dispense with the idea of limiting the solutions to just the approximated ones, and instead favor an approach that could potentially lead to an exhaustive (exponential-time) search of all possible layouts. Its computational complexity thus must be tamed through a constrained search (Branch-and-Bound) and quick identification and pruning of implausible overlays. For his purpose, such a method necessarily relies on a set of score functions (oracles) that can combine different structural properties (e.g. transitivity, coverage, physical maps, etc.). We give a detailed description of this novel assembly framework, referred to as Scoring-and-Unfolding Trimmed Tree Assembler (SUTTA), and present experimental results on several bacterial genomes using next-generation sequencing technology data. We also report experimental evidence that the assembly quality strongly depends on the choice of the minimum overlap parameter k. SUTTA's binaries are freely available to non-profit institutions for research and educational purposes at http://www.bioinformatics.nyu.edu.

Reducing hospital-acquired pressure ulcers using bundle methodology in pediatric and neonatal patients receiving extracorporeal membrane oxygenation therapy: An integrative review and call to action.

Science.gov (United States)

Courtwright, Suzanne E; Mastro, Kari A; Preuster, Christa; Dardashti, Navid; McGill, Sandra; Madelon, Myrlene; Johnson, Donna

2017-10-01

This review focuses on identifying (1) evidence of the effectiveness of care bundle methodology to reduce hospital-acquired pressure ulcers (HAPUs) in pediatric and neonatal patients receiving extracorporeal membrane oxygenation (ECMO) therapy and (2) barriers to implementing HAPU care bundles in this at-risk population. An integrative review was conducted and reported following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines. A search of the scientific literature was performed. Studies included were published between January 2011 and February 2016. A total of seven articles met inclusion criteria. Data were extracted from each published article and analyzed to identify common themes, specifically bundle methodology and barriers to implementing HAPU bundles, in this population. There is limited research on effectiveness of care bundle methodology in reducing HAPUs in children, and no research specific to its effectiveness in pediatric or neonatal ECMO patients. No research was identified studying barriers to implementation of HAPU care bundles in this population. Nurses are well poised to test innovative strategies to prevent HAPUs. Nurses should consider implementing and testing bundle methodology to reduce HAPU in this at-risk population, and conduct research to identify any barriers to implementing this strategy. There is literature to support the use of nurses as unit-based skin care champions to facilitate teamwork and reliable use of the bundle, both critical components to the success of bundle methodology. © 2017 Wiley Periodicals, Inc.