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Sample records for innovative cloning method

  1. Piglets born from handmade cloning, an innovative cloning method without micromanipulation

    DEFF Research Database (Denmark)

    Du, Y.; Kragh, P.M.; Zhang, Y.

    2007-01-01

    Porcine handmade cloning (HMC), a simplified alternative of micromanipulation based traditional cloning (TC) has been developed in multiple phases during the past years, but the final evidence of its biological value, births of piglets was missing. Here we report the first births of healthy piglets......) of HMC reconstructed embryos developed to blastocysts with an average cell number of 77 ± 3 (n = 26) after 7 days in vitro culture (IVC). According to our knowledge, this is the highest in vitro developmental rate after porcine somatic cell nuclear transfer (SCNT). A total of 416 blastocysts from HMC......, mixed with 150 blastocysts from TC using a cell line from a different breed were transferred surgically to nine synchronized recipients. Out of the four pregnancies (44.4%) two were lost, while two pregnancies went to term and litters of 3 and 10 piglets were delivered by Caesarean section, with live...

  2. Automated cloning methods.; TOPICAL

    International Nuclear Information System (INIS)

    Collart, F.

    2001-01-01

    Argonne has developed a series of automated protocols to generate bacterial expression clones by using a robotic system designed to be used in procedures associated with molecular biology. The system provides plate storage, temperature control from 4 to 37 C at various locations, and Biomek and Multimek pipetting stations. The automated system consists of a robot that transports sources from the active station on the automation system. Protocols for the automated generation of bacterial expression clones can be grouped into three categories (Figure 1). Fragment generation protocols are initiated on day one of the expression cloning procedure and encompass those protocols involved in generating purified coding region (PCR)

  3. Cloning

    Science.gov (United States)

    Cloning describes the processes used to create an exact genetic replica of another cell, tissue or organism. ... named Dolly. There are three different types of cloning: Gene cloning, which creates copies of genes or ...

  4. MEANS AND METHODS FOR CLONING NUCLEIC ACID SEQUENCES

    NARCIS (Netherlands)

    Geertsma, Eric Robin; Poolman, Berend

    2008-01-01

    The invention provides means and methods for efficiently cloning nucleic acid sequences of interest in micro-organisms that are less amenable to conventional nucleic acid manipulations, as compared to, for instance, E.coli. The present invention enables high-throughput cloning (and, preferably,

  5. Genetically Modified GMDH Method with Cloning

    Czech Academy of Sciences Publication Activity Database

    Jiřina, Marcel; Jiřina jr., M.

    2007-01-01

    Roč. 28, - (2007), s. 29-37 ISSN 1870-4069. [NNAM 2007. International Conference on Neural Networks and Associative Memories /2./. Mexico City, 04.11.2007-09.11.2007] R&D Projects: GA MŠk(CZ) 1M0567 Institutional research plan: CEZ:AV0Z10300504 Keywords : GMDH neural network * genetic selection * cloning * Machine Learning Repository Subject RIV: BA - General Mathematics

  6. INNOVATIVE SALES METHODS

    Directory of Open Access Journals (Sweden)

    Roxana L. IONESCU

    2014-06-01

    Full Text Available Companies operating in a global economy that is constantly changing and developming, especially during the financial crisis and political instability. It is necessary to adapt and develop sales methods in such environment. For large companies who base their activity on sales it has become a necessity to learn different types of sales approaches because their knowledge enables them to grow the number of customers and therefore the sales and the turnover. This paper aims to exame the most effective sales methods used on the highly sensitive economic and social environment – the insurance market. In the field of insurances, the sales process is even more important because sellers need to sell an intangible product that may materialize in the future, but there is no certainty.

  7. Innovative methods for knowledge transfer

    Science.gov (United States)

    Goanta, A. M.

    2015-11-01

    At this time, learning takes place, either with classic books on paper support or using books scanned or drawn and further converted into PDF or PPT files that are printed on type support CD / DVD. The latter modern means of learning, the study adds live on the Internet using search engines and not least e-learning method, which allows the study of bibliographic related materials in PDF or PPT, stacked and grouped on the basis of a curriculum imposed which can be accessed on a website via a user name and password. Innovative methods come to successfully use other file types than those mentioned above. The graphics in teaching technical subjects such as descriptive geometry can be achieved using animated PowerPoint files, allowing for visualization of steps to be taken, in the case of solving by drawing a descriptive geometry. Another innovative method relies on the use of HTML files, inspired by related sites help design software packages that can be used when teaching descriptive geometry that the technical design. Through this work, the author has proposed to present a new innovative method, which is inspired by the methods listed above, but involves using AVI files to teaching of computer-assisted type graphics or info graphics. In general this new author's method lends itself particularly well to the teaching of the use of software packages because the student actually see the place from where the delivered command is accessed and contextual options of right button of the mouse. These laboratory courses or mentoring can be freestanding cards support CD / DVD or can be posted on websites with restricted access based on user name and password. Practically paper presents the methodology of creating courses and tutorials in AVI format, and how to monitor the degree of accessing the website, on which there were posted mentioned teaching materials, using the tools offered by Google Analytics. The results consist of graphical work, about the degree of access to

  8. FastCloning: a highly simplified, purification-free, sequence- and ligation-independent PCR cloning method

    Directory of Open Access Journals (Sweden)

    Lu Jia

    2011-10-01

    Full Text Available Abstract Background Although a variety of methods and expensive kits are available, molecular cloning can be a time-consuming and frustrating process. Results Here we report a highly simplified, reliable, and efficient PCR-based cloning technique to insert any DNA fragment into a plasmid vector or into a gene (cDNA in a vector at any desired position. With this method, the vector and insert are PCR amplified separately, with only 18 cycles, using a high fidelity DNA polymerase. The amplified insert has the ends with ~16-base overlapping with the ends of the amplified vector. After DpnI digestion of the mixture of the amplified vector and insert to eliminate the DNA templates used in PCR reactions, the mixture is directly transformed into competent E. coli cells to obtain the desired clones. This technique has many advantages over other cloning methods. First, it does not need gel purification of the PCR product or linearized vector. Second, there is no need of any cloning kit or specialized enzyme for cloning. Furthermore, with reduced number of PCR cycles, it also decreases the chance of random mutations. In addition, this method is highly effective and reproducible. Finally, since this cloning method is also sequence independent, we demonstrated that it can be used for chimera construction, insertion, and multiple mutations spanning a stretch of DNA up to 120 bp. Conclusion Our FastCloning technique provides a very simple, effective, reliable, and versatile tool for molecular cloning, chimera construction, insertion of any DNA sequences of interest and also for multiple mutations in a short stretch of a cDNA.

  9. Football refereeing: Identifying innovative methods

    Directory of Open Access Journals (Sweden)

    Reza MohammadKazemi

    2014-08-01

    Full Text Available The aim of the present study is to identify the potentials innovation in football industry. Data were collected from 10 national and international referees, assistant referees and referees’ supervisors in Iran. In this study, technological innovations are identified that assist better refereeing performances. The analysis revealed a significant relationship between using new technologies and referees ‘performance. The results indicate that elite referees, assistant referees and supervisors agreed to use new technological innovations during the game. According to their comments, this kind of technology causes the referees’ performance development.

  10. Developmental Competence and Epigenetic Profile of Porcine Embryos Produced by Two Different Cloning Methods.

    Science.gov (United States)

    Liu, Ying; Lucas-Hahn, Andrea; Petersen, Bjoern; Li, Rong; Hermann, Doris; Hassel, Petra; Ziegler, Maren; Larsen, Knud; Niemann, Heiner; Callesen, Henrik

    2017-06-01

    The "Dolly" based cloning (classical nuclear transfer, [CNT]) and the handmade cloning (HMC) are methods that are nowadays routinely used for somatic cloning of large domestic species. Both cloning protocols share several similarities, but differ with regard to the required in vitro culture, which in turn results in different time intervals until embryo transfer. It is not yet known whether the differences between cloned embryos from the two protocols are due to the cloning methods themselves or the in vitro culture, as some studies have shown detrimental effects of in vitro culture on conventionally produced embryos. The goal of this study was to unravel putative differences between two cloning methods, with regard to developmental competence, expression profile of a panel of developmentally important genes and epigenetic profile of porcine cloned embryos produced by either CNT or HMC, either with (D5 or D6) or without (D0) in vitro culture. Embryos cloned by these two methods had a similar morphological appearance on D0, but displayed different cleavage rates and different quality of blastocysts, with HMC embryos showing higher blastocyst rates (HMC vs. CNT: 35% vs. 10%, p cloned embryos were similar on D0, but differed on D6. In conclusion, both cloning methods and the in vitro culture may affect porcine embryo development and epigenetic profile. The two cloning methods essentially produce embryos of similar quality on D0 and after 5 days in vitro culture, but thereafter both histone acetylation and gene expression differ between the two types of cloned embryos.

  11. Innovative methods in teaching mathematics

    OpenAIRE

    Чепелева, Т. И.

    2013-01-01

    The report outlines the main directions of innovation in the teaching of higher mathematics at the university. The basic technological approach is suggested when creating lecture presentations such as their color characteristics of fonts, the amount of information on a slide, etc., which is based on teaching experience and is suitable for development of other educational presentations.

  12. Biomimetic design method for innovation and sustainability

    CERN Document Server

    Helfman Cohen, Yael

    2017-01-01

    Presenting a novel biomimetic design method for transferring design solutions from nature to technology, this book focuses on structure-function patterns in nature and advanced modeling tools derived from TRIZ, the theory of inventive problem-solving. The book includes an extensive literature review on biomimicry as an engine of both innovation and sustainability, and discusses in detail the biomimetic design process, current biomimetic design methods and tools. The structural biomimetic design method for innovation and sustainability put forward in this text encompasses (1) the research method and rationale used to develop and validate this new design method; (2) the suggested design algorithm and tools including the Findstructure database, structure-function patterns and ideality patterns; and (3) analyses of four case studies describing how to use the proposed method. This book offers an essential resource for designers who wish to use nature as a source of inspiration and knowledge, innovators and sustain...

  13. An accurate clone-based haplotyping method by overlapping pool sequencing.

    Science.gov (United States)

    Li, Cheng; Cao, Changchang; Tu, Jing; Sun, Xiao

    2016-07-08

    Chromosome-long haplotyping of human genomes is important to identify genetic variants with differing gene expression, in human evolution studies, clinical diagnosis, and other biological and medical fields. Although several methods have realized haplotyping based on sequencing technologies or population statistics, accuracy and cost are factors that prohibit their wide use. Borrowing ideas from group testing theories, we proposed a clone-based haplotyping method by overlapping pool sequencing. The clones from a single individual were pooled combinatorially and then sequenced. According to the distinct pooling pattern for each clone in the overlapping pool sequencing, alleles for the recovered variants could be assigned to their original clones precisely. Subsequently, the clone sequences could be reconstructed by linking these alleles accordingly and assembling them into haplotypes with high accuracy. To verify the utility of our method, we constructed 130 110 clones in silico for the individual NA12878 and simulated the pooling and sequencing process. Ultimately, 99.9% of variants on chromosome 1 that were covered by clones from both parental chromosomes were recovered correctly, and 112 haplotype contigs were assembled with an N50 length of 3.4 Mb and no switch errors. A comparison with current clone-based haplotyping methods indicated our method was more accurate. © The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research.

  14. Developmental competence and epigenetic profile of porcine embryos produced by two different cloning methods

    DEFF Research Database (Denmark)

    Liu, Ying; Lucas-Hahn, Andrea; Petersen, Bjoern

    2017-01-01

    on conventionally produced embryos. The goal of this study was to unravel putative differences between two cloning methods, with regard to developmental competence, expression profile of a panel of developmentally important genes and epigenetic profile of porcine cloned embryos produced by either CNT or HMC, either...

  15. GMDH Method with Genetic Selection Algorithm and Cloning

    Czech Academy of Sciences Publication Activity Database

    Jiřina, Marcel; Jiřina jr., M.

    2013-01-01

    Roč. 23, č. 5 (2013), s. 451-464 ISSN 1210-0552 Institutional support: RVO:67985807 Keywords : multivariate data * GMDH * linear regression * Gauss-Markov conditions * cloning * genetic selection * classification Subject RIV: BB - Applied Statistics, Operational Research Impact factor: 0.412, year: 2013

  16. Innovative Methods: Resources for Research, Publishing, and Teaching.

    Science.gov (United States)

    Gergen, Mary; Chrisler, Joan C.; LoCicero, Alice

    1999-01-01

    Reviews a selection of innovative methods congenial to research in feminist psychology and describes undergraduate and graduate courses that emphasize these methods in their curricula. Contains a bibliography of over 300 entries organized by type of innovative method. (SLD)

  17. Evaluation of the efficiency and utility of recombinant enzyme-free seamless DNA cloning methods

    Directory of Open Access Journals (Sweden)

    Ken Motohashi

    2017-03-01

    Full Text Available Simple and low-cost recombinant enzyme-free seamless DNA cloning methods have recently become available. In vivo Escherichia coli cloning (iVEC can directly transform a mixture of insert and vector DNA fragments into E. coli, which are ligated by endogenous homologous recombination activity in the cells. Seamless ligation cloning extract (SLiCE cloning uses the endogenous recombination activity of E. coli cellular extracts in vitro to ligate insert and vector DNA fragments. An evaluation of the efficiency and utility of these methods is important in deciding the adoption of a seamless cloning method as a useful tool. In this study, both seamless cloning methods incorporated inserting DNA fragments into linearized DNA vectors through short (15–39 bp end homology regions. However, colony formation was 30–60-fold higher with SLiCE cloning in end homology regions between 15 and 29 bp than with the iVEC method using DH5α competent cells. E. coli AQ3625 strains, which harbor a sbcA gene mutation that activates the RecE homologous recombination pathway, can be used to efficiently ligate insert and vector DNA fragments with short-end homology regions in vivo. Using AQ3625 competent cells in the iVEC method improved the rate of colony formation, but the efficiency and accuracy of SLiCE cloning were still higher. In addition, the efficiency of seamless cloning methods depends on the intrinsic competency of E. coli cells. The competency of chemically competent AQ3625 cells was lower than that of competent DH5α cells, in all cases of chemically competent cell preparations using the three different methods. Moreover, SLiCE cloning permits the use of both homemade and commercially available competent cells because it can use general E. coli recA− strains such as DH5α as host cells for transformation. Therefore, between the two methods, SLiCE cloning provides both higher efficiency and better utility than the iVEC method for seamless DNA plasmid

  18. Innovative methods of popularizing technical education

    Science.gov (United States)

    Shkitsa, L. Y.; Panchuk, V. G.; Kornuta, V. A.

    2017-05-01

    There have been analyzed reasons of the loss of technical education’s popularity. Also, the analysis of known educational and production methods, oriented at the innovative model of development of society, was performed. It is stated that the acquisition of 21st century’s skills as a result of competition of technical education are natural for the DIY ideology, which was realized in the institutions like Fab Lab. The new educational strategy, based on project-based learning, is proposed to be implemented as a special laboratory with equipment, which would be a center of innovative development for students at the Technical University. Moreover, the list of projects planned for implementation, that includes not only projects, specific to a particular university, but also projects, demanded by society as a whole, is specified. It is worth to implement trendy projects in the laboratory, such as toy-like, ecological projects; projects of the energy dependence decrease or the energy efficiency increase, modern digital or innovative projects etc. The student should gain knowledge, skills and, possibly, equipment that are available for immediate usage on the labor market or for the realization of his own projects or the community’s projects in everyday life after the realization of the particular project at the laboratory

  19. Methods of Profile Cloning Detection in Online Social Networks

    Directory of Open Access Journals (Sweden)

    Zabielski Michał

    2016-01-01

    Full Text Available With the arrival of online social networks, the importance of privacy on the Internet has increased dramatically. Thus, it is important to develop mechanisms that will prevent our hidden personal data from unauthorized access and use. In this paper an attempt was made to present a concept of profile cloning detection in Online Social Networks (OSN using Graph and Networks Theory. By analysing structural similarity of network and value of attributes of user personal profile, we will be able to search for attackers which steal our identity.

  20. Innovation

    DEFF Research Database (Denmark)

    Nielsen, Janni; Yaganeh, Suzanne; Bloch Rasmussen, Leif

    2013-01-01

    This paper contributes to a theoretical discussion of creation of innovation with participants in, or outside, organisations. We address the creation of innovation with a complex theoretical understanding drawing on the Scandinavian and the Participatory Design tradition introducing two approaches...... to the processes of innovation. We ask if innovation can be initiated and enhanced looking at two collaborative approaches; participatory innovation (PIN) and cooperative innovation (COIN). We invite to dialogue and reflections on PIN’s conflict and creative frictions on one side and COIN’s complexity......, complementarity in diversity and the didactic scaffolding of the innovation process on the other side. Our contribution focuses on the methods and practices for facilitation of co-creating activities between different groups leading to cooperation, and innovation in thinking....

  1. Design theory methods and organization for innovation

    CERN Document Server

    Le Masson, Pascal; Hatchuel, Armand

    2017-01-01

    This textbook presents the core of recent advances in design theory and its implications for design methods and design organization. Providing a unified perspective on different design methods and approaches, from the most classic (systematic design) to the most advanced (C-K theory), it offers a unique and integrated presentation of traditional and contemporary theories in the field. Examining the principles of each theory, this guide utilizes numerous real life industrial applications, with clear links to engineering design, industrial design, management, economics, psychology and creativity. Containing a section of exams with detailed answers, it is useful for courses in design theory, engineering design and advanced innovation management. "Students and professors, practitioners and researchers in diverse disciplines, interested in design, will find in this book a rich and vital source for studying fundamental design methods and tools as well as the most advanced design theories that work in practice". Pro...

  2. Innovative design method of automobile profile based on Fourier descriptor

    Science.gov (United States)

    Gao, Shuyong; Fu, Chaoxing; Xia, Fan; Shen, Wei

    2017-10-01

    Aiming at the innovation of the contours of automobile side, this paper presents an innovative design method of vehicle side profile based on Fourier descriptor. The design flow of this design method is: pre-processing, coordinate extraction, standardization, discrete Fourier transform, simplified Fourier descriptor, exchange descriptor innovation, inverse Fourier transform to get the outline of innovative design. Innovative concepts of the innovative methods of gene exchange among species and the innovative methods of gene exchange among different species are presented, and the contours of the innovative design are obtained separately. A three-dimensional model of a car is obtained by referring to the profile curve which is obtained by exchanging xenogeneic genes. The feasibility of the method proposed in this paper is verified by various aspects.

  3. From new clones to flowers – innovative business models in the Hungarian wine economy

    Directory of Open Access Journals (Sweden)

    Kismarjai Balázs

    2017-01-01

    Full Text Available Due to the ovestocked Hungarian wine sector wineries often need to find entirely new ways that enable them to increase their market share. These opportunities are determined by the current market potentials, the geographic location and of course the wine district itself. In this study I examined some examples of outbreak opportunities for producers in this difficult economic situation. Young winemakers from different Hungarian wine districts unite to open wine bars - these are now present in several towns all over the country. Other winemakers have been experimenting with new clones in less recognized wine districts. A small winery from a historic wine district recommends its wines with flowers in a new shop.

  4. Establishment of primary bovine intestinal epithelial cell culture and clone method.

    Science.gov (United States)

    Zhan, Kang; Lin, Miao; Liu, Ming-Mei; Sui, Yang-Nan; Zhao, Guo-Qi

    2017-01-01

    The aim of this study was to establish bovine intestinal epithelial cell (BIEC) line and provide a novel clone cell method. Although various strategies of bovine cell culture and clone techniques have been reported, these methods remain not established. Here, we culture successfully primary BIECs and establish a novel clone cell method. Our result showed that BIECs could be successfully cultured and passaged about generation 5. These cellular aggregates and clusters were adherent loosely at day 2 of culture. Cell aggregates and clusters start to proliferate after approximately 4 d. The BIECs showed positive reaction against cytokeratin 18, E-cadherin, and characteristics of epithelial-like morphology. In addition, the fatty acid-binding proteins (FABPs), villin, and intestinal peptidase (IP) band were positive in BIECs. Our results suggest that the establishment of culturing and clone BIEC methods will apply to isolate and clone other primary cells. These BIECs could therefore contribute to the study of bovine intestinal nutrient absorption and regulation, immune regulation, and the pathogenesis of the bovine intestinal disease, which will provide intestinal cell model in vitro.

  5. Forms and Methods of Agricultural Sector Innovative Activity Improvement

    Directory of Open Access Journals (Sweden)

    Aisha S. Ablyaeva

    2013-01-01

    Full Text Available The article is focused on basic forms and methods to improve the efficiency of innovative activity in the agricultural sector of Ukraine. It was determined that the development of agriculture in Ukraine is affected by a number of factors that must be considered to design innovative models of entrepreneurship development and ways to improve the efficiency of innovative entrepreneurship activity.

  6. An assessment method for system innovation and transition (AMSIT)

    NARCIS (Netherlands)

    Bos, Marc W.; Hofman, Erwin; Kuhlmann, Stefan

    2016-01-01

    To address comprehensive system innovations that may occur in a future transition, a suitable ex ante assessment method is required. The technological innovation system approach is useful for the retrospective study of the conditions for success or failure of innovation trajectories, and the

  7. Efficiency of two enucleation methods connected to handmade cloning to produce transgenic porcine embryos

    DEFF Research Database (Denmark)

    Li, J; Villemoes, K; Zhang, Y

    2009-01-01

    The purpose of our work was to establish an efficient-oriented enucleation method to produce transgenic embryos with handmade cloning (HMC). After 41â€"42 h oocytes maturation, the oocytes were further cultured with or without 0.4 μg/ml demecolcine for 45 min [chemically assisted handmade...... cytoplasts without extrusion cones or PB were selected as recipients. Two cytoplasts were electrofused with one transgenic fibroblasts expressing green fluorescent protein (GFP), while non-transgenic fibroblasts were used as controls. Reconstructed embryos were cultured in Well of Wells (WOWs) with porcine......%) of cloned embryos with GFP transgenic fibroblast cells after CAHE vs OHE. With adjusted time-lapse for zonae-free cloned embryos cultured in WOWs with PZM-3, it was obvious that in vitro developmental competence after CAHE was compromised when compared with the OHE method. OHE enucleation method seems...

  8. A method for autoradiographic studies of single clones of plaque forming cells

    International Nuclear Information System (INIS)

    Andersen, V.; Lefkovits, I.; Rigshospitalet, Copenhagen

    1977-01-01

    By limiting dilution of B lymphocytes from spleens of immunized mice, microcultures were obtained that contained only one clone of plaque forming cells (PFC). The cultured cells were labelled with [ 14 C]thymidine for varying period of time. Plaques were obtained in monolayers of sheep erythrocytes in plastic dishes. After fixation with glutaraldehyde, the bottoms of the dishes were stripped off and autoradiograms prepared. By this method, it is possible to determine the proportion of labelled PFC within a given clone and to quantitate the incorporation of label. The method described can be applied to study the incorporation of other labelled molecules and for cytochemical investigations

  9. The Methodical Approaches to Activation of Innovative Potential of Enterprise

    Directory of Open Access Journals (Sweden)

    Berveno Oksana V.

    2017-12-01

    Full Text Available The article is aimed at developing theoretical provisions and practical recommendations on methods of management and activation of innovative potential of enterprises. Assessment of innovative potential of enterprise should be carried out from different positions, taking into consideration all external and internal possibilities of enterprise as to carrying out an innovation activity. The system of management of innovation activity and implementation of innovative potential at enterprise should be closely woven in the general management of the enterprise. Activation of innovative potential of enterprise foresees adoption of the whole system of strategic decisions, which are aimed at creation of the most favorable conditions for implementation of innovative potential with obtaining of planned results. The system of activization of innovative potential should develop a number of organizational decisions on interaction of elements of the most innovative potential in the process of innovation activity and cooperation of innovative potential with other subsystems of the enterprise. Results of these organizational decisions in many respects determine efficiency of innovation activity of the enterprise.

  10. Bridging Technometric Method and Innovation Process: An Initial Study

    Science.gov (United States)

    Rumanti, A. A.; Reynaldo, R.; Samadhi, T. M. A. A.; Wiratmadja, I. I.; Dwita, A. C.

    2018-03-01

    The process of innovation is one of ways utilized to increase the capability of a technology component that reflects the need of SME. Technometric method can be used to identify to what extent the level of technology advancement in a SME is, and also which technology component that needs to be maximized in order to significantly deliver an innovation. This paper serves as an early study, which lays out a conceptual framework that identifies and elaborates the principles of innovation process from a well-established innovation model by Martin with the technometric method, based on the initial background research conducted at SME Ira Silver in Jogjakarta, Indonesia.

  11. Proposition of a method to formulate idea and innovation problems

    Directory of Open Access Journals (Sweden)

    Stéphane GORIA

    2010-01-01

    Full Text Available Nowadays, innovation is a major stake for development and durability of many companies and institution’s activities. In our work, we are interested in interpersonal communication problem in innovation context. We focus on the upstream of innovation process and we consider the means that can be used. First to leverage organisation’s innovation perspective and second to solve innovation problem proposed by a strategic decision maker. To do this, we believe that people charge with solving an innovation problem should manage some difficulties: to identify what can be an innovation for the decision maker, to define the objects with a big innovation potential, communicate to his possible partners the innovation field to investigate and to find ideas to innovate etc. Then, we try to identify and to present these ideas to contribute to decision making process. At this time when the Web is really the universal source with access to formidable information quantities, we use it to establish some creativity and idea sources for innovation. This paper presents a method to solve these communication problems. It presents a tool box which potentialities are illustrated by an example of the management of a “new chair” development problem.

  12. Method of oocyte activation affects cloning efficiency in pigs.

    Science.gov (United States)

    Whitworth, Kristin M; Li, Rongfeng; Spate, Lee D; Wax, David M; Rieke, August; Whyte, Jeffrey J; Manandhar, Gaurishankar; Sutovsky, Miriam; Green, Jonathan A; Sutovsky, Peter; Prather, Randall S

    2009-05-01

    The following experiments compared the efficiency of three fusion/activation protocols following somatic cell nuclear transfer (SCNT) with porcine somatic cells transfected with enhanced green fluorescent protein driven by the chicken beta-actin/rabbit beta-globin hybrid promoter (pCAGG-EGFP). The three protocols included electrical fusion/activation (NT1), electrical fusion/activation followed by treatment with a reversible proteasomal inhibitor MG132 (NT2) and electrical fusion in low Ca(2+) followed by chemical activation with thimerosal/dithiothreitol (NT3). Data were collected at Days 6, 12, 14, 30, and 114 of gestation. Fusion rates, blastocyst-stage mean cell numbers, recovery rates, and pregnancy rates were calculated and compared between protocols. Fusion rates were significantly higher for NT1 and NT2 compared to NT3 (P NT1 (71.4%, n = 28; P 0.05). All fusion/activation treatments produced live, pCAGG-EGFP positive piglets from SCNT. Treatment with MG132 after fusion/activation of reconstructed porcine embryos was the most effective method when comparing the overall pregnancy rates. The beneficial effect of NT2 protocol may be due to the stimulation of proteasomes that infiltrate donor cell nucleus shortly after nuclear transfer. (c) 2008 Wiley-Liss, Inc.

  13. AFEAP cloning: a precise and efficient method for large DNA sequence assembly.

    Science.gov (United States)

    Zeng, Fanli; Zang, Jinping; Zhang, Suhua; Hao, Zhimin; Dong, Jingao; Lin, Yibin

    2017-11-14

    Recent development of DNA assembly technologies has spurred myriad advances in synthetic biology, but new tools are always required for complicated scenarios. Here, we have developed an alternative DNA assembly method named AFEAP cloning (Assembly of Fragment Ends After PCR), which allows scarless, modular, and reliable construction of biological pathways and circuits from basic genetic parts. The AFEAP method requires two-round of PCRs followed by ligation of the sticky ends of DNA fragments. The first PCR yields linear DNA fragments and is followed by a second asymmetric (one primer) PCR and subsequent annealing that inserts overlapping overhangs at both sides of each DNA fragment. The overlapping overhangs of the neighboring DNA fragments annealed and the nick was sealed by T4 DNA ligase, followed by bacterial transformation to yield the desired plasmids. We characterized the capability and limitations of new developed AFEAP cloning and demonstrated its application to assemble DNA with varying scenarios. Under the optimized conditions, AFEAP cloning allows assembly of an 8 kb plasmid from 1-13 fragments with high accuracy (between 80 and 100%), and 8.0, 11.6, 19.6, 28, and 35.6 kb plasmids from five fragments at 91.67, 91.67, 88.33, 86.33, and 81.67% fidelity, respectively. AFEAP cloning also is capable to construct bacterial artificial chromosome (BAC, 200 kb) with a fidelity of 46.7%. AFEAP cloning provides a powerful, efficient, seamless, and sequence-independent DNA assembly tool for multiple fragments up to 13 and large DNA up to 200 kb that expands synthetic biologist's toolbox.

  14. Development of Methods of Innovative Projects’ Management in Developer Organizations

    Directory of Open Access Journals (Sweden)

    Papelniuk Oksana

    2017-01-01

    Full Text Available In article the author studies the conceptual scheme of life cycle of innovative developer project and offers methods of innovative projects' management according to the stages of its lifecycle. The author describes algorithm of the organization of management system of innovative projects in the developer organization representing set of stages and methods. The offered algorithm allows developer organizations achieve one of the main of their objectives, which is the creation of a special environment of comfortable accommodation with the emphasis on ecological compatibility and energy efficiency.

  15. Multiple Site-Directed and Saturation Mutagenesis by the Patch Cloning Method.

    Science.gov (United States)

    Taniguchi, Naohiro; Murakami, Hiroshi

    2017-01-01

    Constructing protein-coding genes with desired mutations is a basic step for protein engineering. Herein, we describe a multiple site-directed and saturation mutagenesis method, termed MUPAC. This method has been used to introduce multiple site-directed mutations in the green fluorescent protein gene and in the moloney murine leukemia virus reverse transcriptase gene. Moreover, this method was also successfully used to introduce randomized codons at five desired positions in the green fluorescent protein gene, and for simple DNA assembly for cloning.

  16. APPLICATION OF QUALITY ECONOMY METHODS IN MANAGING INNOVATION CAPACITY DEVELOPMENT

    Directory of Open Access Journals (Sweden)

    V. V. Okrepilov

    2017-01-01

    Full Text Available Purpose: to reveal the possibilities of applying the methods of the quality economy to improve the management efficiency of the development of the region's innovative potential.Results: in the article topical questions of development of innovative potential of the region are considered - one of the important factors providing improvement of quality of life of the population and sustainable development of the territory. The content of the concept of "innovation potential of the region" is disclosed and its components are described. The purposes of development of innovative potential are considered. A comparative analysis of the methods of managing the development of potential in the market and planned economy is carried out. Particular attention is paid to the application of quality management methods to improve management effectiveness. In conclusion, the advantages and disadvantages of the economy of the macro-region "North-West" from the point of view of transition to an innovative development path, as well as scenarios for the development of the economy, are discussed.Conclusions and relevance: the use of quality economy tools to manage the development of innovative capacity will help to avoid mistakes and choose the right directions for development. In the era of globalization, improving quality is a prerequisite for successful competition in world markets. Therefore, the issues of innovation capacity assessment should be taken into account in the development of regional plans and programs, as well as socio-economic policy.

  17. TRANSFORMATION OF INNOVATIVE AND METHODICAL ACTIVITY LIBRARY OF THE UNIVERSITY

    Directory of Open Access Journals (Sweden)

    О. О. Скаченко

    2015-09-01

    Full Text Available The purpose of our article is the analysis of innovative and methodical activity of university libraries which develop as information centers today. The subject of research is methodical, publishing and innovative activity of Scientific Library of the Kiev national university of culture and arts. We observe process of introduction of technological innovations in library service that allows improving quality of the services provided to the reader by library. New actual projects are developed, cultural and educational and information services extend, work methods improve. Also, the structure of library is improved – there are new sectors. The main finding of the work consists in systematization of various aspects and the directions of innovative activity of library. The research findings have the practical value for library workers, teachers of university, students, library users, and also for anyone who is interested in library science.

  18. MINOR INNOVATIVE ENTERPRISES AND METHODS OF THEIR FINANCING IN RUSSIA

    Directory of Open Access Journals (Sweden)

    A. G. Komissarov

    2011-01-01

    Full Text Available Minor enterprises do not have resources sufficient for investment. Present experience of venture financing and attraction of funds from finance markets is poor in Russia. Main minor entrepreneurship financing sources are state allocations in the form of subsidies, subventionsand donations. This support promotes economics diversification and increases jobs. The state becomes client of innovations and provides for business environment favorable for minor entrepreneurship and for subsequent diffusion of innovations. One of most efficient innovationactivity stimulation methods (applied to minor enterprises as well is provision for tax privileges, favorable state bank credits and preferences to innovation activity supporting commercial banks.

  19. Dog cloning with in vivo matured oocytes obtained using electric chemiluminescence immunoassay-predicted ovulation method.

    Science.gov (United States)

    Lee, Seunghoon; Zhao, Minghui; No, Jingu; Nam, Yoonseok; Im, Gi-Sun; Hur, Tai-Young

    2017-01-01

    Radioactive immunoassay (RIA) is a traditional serum hormone assay method, but the application of the method in reproductive studies is limited by the associated radioactivity. The aim of present study was to evaluate the reliability of RIA and to compare its canine serum progesterone concentration determination accuracy to that of the electric chemiluminescence immunoassay (ECLI). In vivo matured oocytes were utilized for canine somatic cell nuclear transfer (SCNT), and serum progesterone levels were assessed to accurately determine ovulation and oocyte maturation. Canine serum progesterone concentrations during both proestrus and estrus were analyzed by RIA and ECLI to determine the ovulation day. Although both methods detected similar progesterone levels before ovulation, the mean progesterone concentration determined using ECLI was significantly higher than of RIA three days before ovulation. Following ovulation, oocytes were collected by surgery, and a lower percentage of mature oocytes were observed using ECLI (39%) as compared to RIA (67%) if 4-8ng/ml of progesterone were used for determination of ovulation. A high percentage of mature oocytes was observed using ECLI when 6-15 ng/mL of progesterone was used for ovulation determination. To determine whether ECLI could be used for canine cloning, six canines were selected as oocyte donors, and two puppies were obtained after SCNT and embryo transfer. In conclusion, compared to the traditional RIA method, the ECLI method is a safe and reliable method for canine cloning.

  20. Cost Cutting in Hospitals. Innovative Methods & Techniques

    OpenAIRE

    Pandit, Abhijit

    2016-01-01

    There is emerging need of hospitals to address efficiency issues confronting health care reforms in the present environment. The main objective is to investigate the cost efficiency of hospitals using various methods and variables, and compare the results estimated by the different methods and variables. Reinforcing a common agenda between medical, paramedical and administrative staff, and sharing a common vision among professionals and decision makers in the planning of care, may be the grea...

  1. Methods and tools for innovation management procuring enterprises

    OpenAIRE

    A. V. Bogomolov

    2013-01-01

    The traditional methods of quality control of grain, dock-led feasibility, proposed and tested in practice near-mill elevator innovative methods of management organization, introduction of resource-saving technologies, different allow using «piezo electronic sensor» more quickly and accurately detect early damage of grain of wheat and ensure its further storage without loss of quality are described.

  2. Different Donor Cell Culture Methods Can Influence the Developmental Ability of Cloned Sheep Embryos.

    Directory of Open Access Journals (Sweden)

    LiBing Ma

    Full Text Available It was proposed that arresting nuclear donor cells in G0/G1 phase facilitates the development of embryos that are derived from somatic cell nuclear transfer (SCNT. Full confluency or serum starvation is commonly used to arrest in vitro cultured somatic cells in G0/G1 phase. However, it is controversial as to whether these two methods have the same efficiency in arresting somatic cells in G0/G1 phase. Moreover, it is unclear whether the cloned embryos have comparable developmental ability after somatic cells are subjected to one of these methods and then used as nuclear donors in SCNT. In the present study, in vitro cultured sheep skin fibroblasts were divided into four groups: (1 cultured to 70-80% confluency (control group, (2 cultured to full confluency, (3 starved in low serum medium for 4 d, or (4 cultured to full confluency and then further starved for 4 d. Flow cytometry was used to assay the percentage of fibroblasts in G0/G1 phase, and cell counting was used to assay the viability of the fibroblasts. Then, real-time reverse transcription PCR was used to determine the levels of expression of several cell cycle-related genes. Subsequently, the four groups of fibroblasts were separately used as nuclear donors in SCNT, and the developmental ability and the quality of the cloned embryos were compared. The results showed that the percentage of fibroblasts in G0/G1 phase, the viability of fibroblasts, and the expression levels of cell cycle-related genes was different among the four groups of fibroblasts. Moreover, the quality of the cloned embryos was comparable after these four groups of fibroblasts were separately used as nuclear donors in SCNT. However, cloned embryos derived from fibroblasts that were cultured to full confluency combined with serum starvation had the highest developmental ability. The results of the present study indicate that there are synergistic effects of full confluency and serum starvation on arresting fibroblasts in

  3. The laddering method in service innovation research

    DEFF Research Database (Denmark)

    Grünbaum, Niels Nolsøe

    2017-01-01

    The laddering method is a qualitative interview technique applied in a situation with one interviewer and one informant with the aim of creating an understanding of the value that business-to-consumer (B2C) customers extract from product attributes. Thus, this methodology aims to depict a mental ...

  4. Participatory methods for initiating manufacturing employees' involvement in product innovation

    DEFF Research Database (Denmark)

    Jensen, Anna Rose Vagn; Jensen, Christian Schou; Broberg, Ole

    2016-01-01

    approach that we apply to two case companies, this paper presents an empirical study of how to initiate involvement of manufacturing employees in R&D activities. We have used participatory methods from design thinking that has the ability to create relations between employees from different backgrounds......Employee-driven innovation has the potential to improve product innovation by involving employees as innovative resources. However, it can be a challenge to turn the potential into a reality of collaboration practices across organizational structures and culture. Through an interactive research...... and through a series of facilitated workshops we have investigated how these methods can initiate employee involvement. We see that participatory methods can improve understanding and relation between R&D and manufacturing departments, and thereby support a creative collaboration and emergence of employee...

  5. Innovative methods for inorganic sample preparation

    Energy Technology Data Exchange (ETDEWEB)

    Essling, A.M.; Huff, E.A.; Graczyk, D.G.

    1992-04-01

    Procedures and guidelines are given for the dissolution of a variety of selected materials using fusion, microwave, and Parr bomb techniques. These materials include germanium glass, corium-concrete mixtures, and zeolites. Emphasis is placed on sample-preparation approaches that produce a single master solution suitable for complete multielement characterization of the sample. In addition, data are presented on the soil microwave digestion method approved by the Environmental Protection Agency (EPA). Advantages and disadvantages of each sample-preparation technique are summarized.

  6. Innovative methods for inorganic sample preparation

    International Nuclear Information System (INIS)

    Essling, A.M.; Huff, E.A.; Graczyk, D.G.

    1992-04-01

    Procedures and guidelines are given for the dissolution of a variety of selected materials using fusion, microwave, and Parr bomb techniques. These materials include germanium glass, corium-concrete mixtures, and zeolites. Emphasis is placed on sample-preparation approaches that produce a single master solution suitable for complete multielement characterization of the sample. In addition, data are presented on the soil microwave digestion method approved by the Environmental Protection Agency (EPA). Advantages and disadvantages of each sample-preparation technique are summarized

  7. Innovative Methods and Applications in Mucoadhesion Research

    DEFF Research Database (Denmark)

    Mackie, Alan; Goycoolea, Francisco M.; Menchicchi, Bianca

    2017-01-01

    The present review is aimed at elucidating relatively new aspects of mucoadhesion/mucus interaction and related phenomena that emerged from a Mucoadhesion workshop held in Munster on 2–3 September 2015 as a satellite event of the ICCC 13th—EUCHIS 12th. After a brief outline of the new issues......, the focus is on mucus description, purification, and mucus/mucin characterization, all steps that are pivotal to the understanding of mucus related phenomena and the choice of the correct mucosal model for in vitro and ex vivo experiments, alternative bio/mucomimetic materials are also presented....... Then a selection of preparative techniques and testing methods are described (at molecular as well as micro and macroscale) that may support the pharmaceutical development of mucus interactive systems and assist formulators in the scale-up and industrialization steps. Recent applications of mucoadhesive systems...

  8. Innovative electron transport methods in EGS5

    International Nuclear Information System (INIS)

    Bielajew, A.F.; Wilderman, S.J.

    2000-01-01

    The initial formulation of a Monte Carlo scheme for the transport of high-energy (>≅ 100 keV) electrons was established by Berger in 1963. Calling his method the 'condensed history theory', Berger combined the theoretical results of the previous generation of research into developing approximate solutions of the Boltzmann transport equation with numerical algorithms for exploiting the power of computers to permit iterative, piece-wise solution of the transport equation in a computationally intensive but much less approximate fashion. The methods devised by Berger, with comparatively little modification, provide the foundation of all present day Monte Carlo electron transport simulation algorithms. Only in the last 15 years, beginning with the development and publication of the PRESTA algorithm, has there been a significant revisitation of the problem of simulating electron transport within the condensed history framework. Research in this area is ongoing, highly active, and far from complete. It presents an enormous challenge, demanding derivation of new analytical transport solutions based on underlying fundamental interaction mechanisms, intuitive insight in the development of computer algorithms, and state of the art computer science skills in order to permit deployment of these techniques in an efficient manner. The EGS5 project, a modern ground-up rewrite of the EGS4 code, is now in the design phase. EGS5 will take modern photon and electron transport algorithms and deploy them in an easy-to-maintain, modern computer language-ANSI-standard C ++. Moreover, the well-known difficulties of applying EGS4 to practical geometries (geometry code development, tally routine design) should be made easier and more intuitive through the use of a visual user interface being designed by Quantum Research, Inc., work that is presented elsewhere in this conference. This report commences with a historical review of electron transport models culminating with the proposal of a

  9. CLoNe is a new method to target single progenitors and study their progeny in mouse and chick.

    Science.gov (United States)

    García-Moreno, Fernando; Vasistha, Navneet A; Begbie, Jo; Molnár, Zoltán

    2014-04-01

    Cell lineage analysis enables us to address pivotal questions relating to: the embryonic origin of cells and sibling cell relationships in the adult body; the contribution of progenitors activated after trauma or disease; and the comparison across species in evolutionary biology. To address such fundamental questions, several techniques for clonal labelling have been developed, each with its shortcomings. Here, we report a novel method, CLoNe that is designed to work in all vertebrate species and tissues. CLoNe uses a cocktail of labelling, targeting and transposition vectors that enables targeting of specific subpopulations of progenitor types with a combination of fluorophores resulting in multifluorescence that describes multiple clones per specimen. Furthermore, transposition into the genome ensures the longevity of cell labelling. We demonstrate the robustness of this technique in mouse and chick forebrain development, and show evidence that CLoNe will be broadly applicable to study clonal relationships in different tissues and species.

  10. Innovation

    Science.gov (United States)

    EPA frames innovation as critical to the protection of human health and the environment through initiatives such as sustainable practices, innovative research, prize competitions, innovation awards, partnerships, and community activities.

  11. Structured methods for customer-oriented innovation research

    DEFF Research Database (Denmark)

    Scholderer, Joachim

    customers might have told them if they had only properly been asked beforehand. In the following, a short introduction will be given to structured research methods that are relatively easy to use and can help product developers prioritise their efforts and assess the market potential of innovative concepts....

  12. Methods and tools for innovation management procuring enterprises

    Directory of Open Access Journals (Sweden)

    A. V. Bogomolov

    2013-01-01

    Full Text Available The traditional methods of quality control of grain, dock-led feasibility, proposed and tested in practice near-mill elevator innovative methods of management organization, introduction of resource-saving technologies, different allow using «piezo electronic sensor» more quickly and accurately detect early damage of grain of wheat and ensure its further storage without loss of quality are described.

  13. Enzyme free cloning for high throughput gene cloning and expression

    NARCIS (Netherlands)

    de Jong, R.N.; Daniëls, M.; Kaptein, R.; Folkers, G.E.

    2006-01-01

    Structural and functional genomics initiatives significantly improved cloning methods over the past few years. Although recombinational cloning is highly efficient, its costs urged us to search for an alternative high throughput (HTP) cloning method. We implemented a modified Enzyme Free Cloning

  14. Simple and versatile molecular method of copy-number measurement using cloned competitors.

    Directory of Open Access Journals (Sweden)

    Hyun-Kyoung Kim

    Full Text Available Variations and alterations of copy numbers (CNVs and CNAs carry disease susceptibility and drug responsiveness implications. Although there are many molecular methods to measure copy numbers, sensitivity, reproducibility, cost, and time issues remain. In the present study, we were able to solve those problems utilizing our modified real competitive PCR method with cloned competitors (mrcPCR. First, the mrcPCR for ERBB2 copy number was established, and the results were comparable to current standard methods but with a shorter assay time and a lower cost. Second, the mrcPCR assays for 24 drug-target genes were established, and the results in a panel of NCI-60 cells were comparable to those from real-time PCR and microarray. Third, the mrcPCR results for FCGR3A and the FCGR3B CNVs were comparable to those by the paralog ratio test (PRT, but without PRT's limitations. These results suggest that mrcPCR is comparable to the currently available standard or the most sensitive methods. In addition, mrcPCR would be invaluable for measurement of CNVs in genes with variants of similar structures, because combination of the other methods is not necessary, along with its other advantages such as short assay time, small sample amount requirement, and applicability to all sequences and genes.

  15. Innovation camp as a method in health education

    DEFF Research Database (Denmark)

    Duus, Lene; Ringby, Betina

    2017-01-01

    Society calls for creative, innovative and enterprising health professionals who also have abilities to work interdisciplinary. New learning methods are required to develop new skills. Health care students must develop mind-sets and skills that enable them to create, develop and take action upon...... new services in new or existing organisations. Students also need to develop skills for interdisciplinary collaboration and develop their own knowledge of different cultures and behaviours. Therefore, educational institutions must challenge students in many different ways....

  16. Influence of embryo handling and transfer method on pig cloning efficiency.

    Science.gov (United States)

    Shi, Junsong; Zhou, Rong; Luo, Lvhua; Mai, Ranbiao; Zeng, Haiyu; He, Xiaoyan; Liu, Dewu; Zeng, Fang; Cai, Gengyuan; Ji, Hongmei; Tang, Fei; Wang, Qinglai; Wu, Zhenfang; Li, Zicong

    2015-03-01

    The somatic cell nuclear transfer (SCNT) technique could be used to produce genetically superior or genetically engineered cloned pigs that have wide application in agriculture and bioscience research. However, the efficiency of porcine SCNT currently is very low. Embryo transfer (ET) is a key step for the success of SCNT. In this study, the effects of several ET-related factors, including cloned embryo culture time, recipient's ovulation status, co-transferred helper embryos and ET position, on the success rate of pig cloning were investigated. The results indicated that transfer of cloned embryos cultured for a longer time (22-24h vs. 4-6h) into pre-ovulatory sows decreased recipient's pregnancy rate and farrowing rate, and use of pre-ovulatory and post-ovulatory sows as recipients for SCNT embryos cultured for 22-24h resulted in a similar porcine SCNT efficiency. Use of insemination-produced in vivo fertilized, parthenogenetically activated and in vitro fertilized embryos as helper embryos to establish and/or maintain pregnancy of SCNT embryos recipients could not improve the success rate of porcine SCNT. Transfer of cloned embryos into double oviducts of surrogates significantly increased pregnancy rate as well as farrowing rate of recipients, and the developmental rate of transferred cloned embryos, as compared to unilateral oviduct transfer. This study provided useful information for optimization of the embryo handling and transfer protocol, which will help to improve the ability to generate cloned pigs. Copyright © 2015 Elsevier B.V. All rights reserved.

  17. Analysing malaria drug trials on a per-individual or per-clone basis: a comparison of methods.

    Science.gov (United States)

    Jaki, Thomas; Parry, Alice; Winter, Katherine; Hastings, Ian

    2013-07-30

    There are a variety of methods used to estimate the effectiveness of antimalarial drugs in clinical trials, invariably on a per-person basis. A person, however, may have more than one malaria infection present at the time of treatment. We evaluate currently used methods for analysing malaria trials on a per-individual basis and introduce a novel method to estimate the cure rate on a per-infection (clone) basis. We used simulated and real data to highlight the differences of the various methods. We give special attention to classifying outcomes as cured, recrudescent (infections that never fully cleared) or ambiguous on the basis of genetic markers at three loci. To estimate cure rates on a per-clone basis, we used the genetic information within an individual before treatment to determine the number of clones present. We used the genetic information obtained at the time of treatment failure to classify clones as recrudescence or new infections. On the per-individual level, we find that the most accurate methods of classification label an individual as newly infected if all alleles are different at the beginning and at the time of failure and as a recrudescence if all or some alleles were the same. The most appropriate analysis method is survival analysis or alternatively for complete data/per-protocol analysis a proportion estimate that treats new infections as successes. We show that the analysis of drug effectiveness on a per-clone basis estimates the cure rate accurately and allows more detailed evaluation of the performance of the treatment. Copyright © 2012 John Wiley & Sons, Ltd.

  18. An alternative method for cDNA cloning from surrogate eukaryotic cells transfected with the corresponding genomic DNA.

    Science.gov (United States)

    Hu, Lin-Yong; Cui, Chen-Chen; Song, Yu-Jie; Wang, Xiang-Guo; Jin, Ya-Ping; Wang, Ai-Hua; Zhang, Yong

    2012-07-01

    cDNA is widely used in gene function elucidation and/or transgenics research but often suitable tissues or cells from which to isolate mRNA for reverse transcription are unavailable. Here, an alternative method for cDNA cloning is described and tested by cloning the cDNA of human LALBA (human alpha-lactalbumin) from genomic DNA. First, genomic DNA containing all of the coding exons was cloned from human peripheral blood and inserted into a eukaryotic expression vector. Next, by delivering the plasmids into either 293T or fibroblast cells, surrogate cells were constructed. Finally, the total RNA was extracted from the surrogate cells and cDNA was obtained by RT-PCR. The human LALBA cDNA that was obtained was compared with the corresponding mRNA published in GenBank. The comparison showed that the two sequences were identical. The novel method for cDNA cloning from surrogate eukaryotic cells described here uses well-established techniques that are feasible and simple to use. We anticipate that this alternative method will have widespread applications.

  19. On managing innovation by design : Towards SMART methods

    NARCIS (Netherlands)

    Vermaas, P.E.

    2012-01-01

    In this paper innovation by design is characterised schematically and methodological with the aim of analysing how projects of innovation by design can be managed. First, I approach innovation by design from an engineering perspective, and show that the incorporation of innovation into design

  20. Cloning Mice.

    Science.gov (United States)

    Ogura, Atsuo

    2017-08-01

    Viable and fertile mice can be generated by somatic nuclear transfer into enucleated oocytes, presumably because the transplanted somatic cell genome becomes reprogrammed by factors in the oocyte. The first somatic cloned offspring of mice were obtained by directly injecting donor nuclei into recipient enucleated oocytes. When this method is used (the so-called Honolulu method of somatic cell nuclear transfer [SCNT]), the donor nuclei readily and completely condense within the enucleated metaphase II-arrested oocytes, which contain high levels of M-phase-promoting factor (MPF). It is believed that the condensation of the donor chromosomes promotes complete reprogramming of the donor genome within the mouse oocytes. Another key to the success of mouse cloning is the use of blunt micropipettes attached to a piezo impact-driving micromanipulation device. This system saves a significant amount of time during the micromanipulation of oocytes and thus minimizes the loss of oocyte viability in vitro. For example, a group of 20 oocytes can be enucleated within 10 min by an experienced operator. This protocol is composed of seven parts: (1) preparing micropipettes, (2) setting up the enucleation and injection micropipettes, (3) collecting and enucleating oocytes, (4) preparing nucleus donor cells, (5) injecting donor nuclei, (6) activating embryos and culturing, and (7) transferring cloned embryos. © 2017 Cold Spring Harbor Laboratory Press.

  1. What is Cloning?

    Science.gov (United States)

    Donate Home Cloning What is Cloning What is Cloning Clones are organisms that are exact genetic copies. ... clones made through modern cloning technologies. How Is Cloning Done? Many people first heard of cloning when ...

  2. Innovators.

    Science.gov (United States)

    NEA Today, 2001

    2001-01-01

    Describes various innovations that have been developed to enhance education. These innovations include: helping educators help at-risk students succeed; promoting high school journalism; ensuring quality online learning experiences; developing a student performing group that uses theater to address social issues; and having students design their…

  3. Innovative teaching methods for capacity building in knowledge translation

    Directory of Open Access Journals (Sweden)

    Al-Ansary Lubna A

    2011-10-01

    Full Text Available Abstract Background In some current healthcare settings, there is a noticeable absence of national institutions committed to the synthesis and use of evidence in healthcare decision- and policy-making. This absence creates a need to broaden the responsibilities of healthcare providers to include knowledge brokering and advocacy in order to optimize knowledge translation to other stakeholders, especially policy-makers. However, this process requires practitioners and researchers to acquire certain types of knowledge and skills. This article introduces two innovative methods for capacity building in knowledge translation (KT. Methods During a workshop aimed at preparing 21 trainers in evidence-based medicine, two innovative methods were used: (1 debate and (2 a knowledge translation project (KTP. The main objective of the debates approach was to strengthen participants' critical thinking abilities by requiring them to search for and appraise evidence and defend their arguments. The KTP was used to introduce participants to the essential steps of knowledge translation and to suggest an extended role for healthcare practitioners, i.e., using evidence to manage not only individual patients but also to a community of patients. Participants' performances were assessed according to a pre-designed scheme. At the end of the workshop, participants' opinions and experiences with the innovative teaching methods were evaluated based on their answers to a questionnaire and the results of small-group discussions. Results The participants performed well in both the debate and KTP methods. During post-workshop group discussions, they indicated that the debate approach had added a new dimension to their evidence-based medicine skills by adding purpose and motivation. However, they felt that their performances would have been better if they had been offered practical demonstrations of how to conduct the debate. The participants indicated that the KTP enhanced their

  4. Innovative teaching methods for capacity building in knowledge translation.

    Science.gov (United States)

    Wahabi, Hayfaa A; Al-Ansary, Lubna A

    2011-10-14

    In some current healthcare settings, there is a noticeable absence of national institutions committed to the synthesis and use of evidence in healthcare decision- and policy-making. This absence creates a need to broaden the responsibilities of healthcare providers to include knowledge brokering and advocacy in order to optimize knowledge translation to other stakeholders, especially policy-makers. However, this process requires practitioners and researchers to acquire certain types of knowledge and skills. This article introduces two innovative methods for capacity building in knowledge translation (KT). During a workshop aimed at preparing 21 trainers in evidence-based medicine, two innovative methods were used: (1) debate and (2) a knowledge translation project (KTP). The main objective of the debates approach was to strengthen participants' critical thinking abilities by requiring them to search for and appraise evidence and defend their arguments. The KTP was used to introduce participants to the essential steps of knowledge translation and to suggest an extended role for healthcare practitioners, i.e., using evidence to manage not only individual patients but also to a community of patients. Participants' performances were assessed according to a pre-designed scheme. At the end of the workshop, participants' opinions and experiences with the innovative teaching methods were evaluated based on their answers to a questionnaire and the results of small-group discussions. The participants performed well in both the debate and KTP methods. During post-workshop group discussions, they indicated that the debate approach had added a new dimension to their evidence-based medicine skills by adding purpose and motivation. However, they felt that their performances would have been better if they had been offered practical demonstrations of how to conduct the debate. The participants indicated that the KTP enhanced their understanding of the relationships between evidence

  5. Innovation

    African Journals Online (AJOL)

    In recent years ULA has emphasized advocacy, and contributed to progress towards new legislation (freedom of information, copyright, the ... East African Community e-government strategy) of importance to the library and ... Innovation Vol.

  6. Study of the efficiency of chemically assisted enucleation method for handmade cloning in goat (Capra hircus).

    Science.gov (United States)

    Akshey, Y S; Malakar, D; De, A K; Jena, M K; Sahu, S; Dutta, R

    2011-08-01

    The present investigation was carried out to find an efficient chemically assisted procedure for enucleation of goat oocytes related to handmade cloning (HMC) technique. After 22-h in vitro maturation, oocytes were incubated with 0.5 μg/ml demecolcine for 2 h. Cumulus cells were removed by pipetting and vortexing in 0.5 mg/ml hyaluronidase, and zona pellucida were digested with pronase. Oocytes with extrusion cones were subjected to oriented bisection. One-third of the cytoplasm with the extrusion cone was removed with a micro blade. The remaining cytoplasts were used as recipients in HMC. Goat foetal fibroblasts were used as nuclear donors. The overall efficiency measured as the number of cytoplasts obtained per total number of oocytes used was significantly (p < 0.05) higher in chemically assisted handmade enucleation (CAHE) than oriented handmade enucleation without demecolcine (OHE) (80.02 ± 1.292% vs. 72.9 ± 1.00%, respectively, mean ± SEM). The reconstructed and activated embryos were cultured in embryo development medium (EDM) for 7 days. Fusion, cleavage and blastocyst development rate were 71.63 ± 1.95%, 92.94 ± 0.91% and 23.78 ± 3.33% (mean ± SEM), respectively which did not differ significantly from those achieved with random handmade enucleation and OHE. In conclusion, chemically assisted enucleation is a highly efficient and reliable enucleation method for goat HMC which eliminates the need of expensive equipment (inverted fluorescence microscope) and potentially harmful chromatin staining and ultraviolet (UV) irradiation for cytoplast selection. © 2010 Blackwell Verlag GmbH.

  7. A Novel Loop-Mediated Isothermal Amplification (LAMP) Method for Detection of the JP2 Clone of Aggregatibacter actinomycetemcomitans in Subgingival Plaque

    DEFF Research Database (Denmark)

    Seki, M; Poulsen, Knud; Haubek, Dorte

    2008-01-01

    We developed a LAMP method that detects the JP2 clone of Aggregatibacter actinomycetemcomitans, which induces aggressive periodontitis in adolescents of North- and West-African descents. Being independent of special equipment this specific and sensitive method offers significant advantages...

  8. Innovation of fission gas release and thermal conductivity measurement methods

    International Nuclear Information System (INIS)

    Van der Meer, K.; Soboler, V.

    1998-01-01

    This presentation described two innovative measurement methods being currently developed at SCK-CEN in order to support the modeling of fuel performance. The first one is an acoustic method to measure the fission gas release in a fuel rod in a non destructive way. The total rod pressure is determined by generating a heat pulse causing a pressure wave that propagates through the gas to an ultrasound transducer. The final pulse width being proportional to the pressure, the latter can thus be determined. The measurement of the acoustic resonance frequency at fixed temperatures enables the distinction between different gas components. The second method is a non-stationary technique to investigate the thermal properties of the fuel rod, like thermal conductivity, diffusivity and heat capacity. These properties are derived from the amplitude and the phase shift of the fuel centre temperature response induced by a periodic temperature variation. These methods did not reveal any physical limitations for the practical applicability. Furthermore, they are rather simple. Preliminary investigations have proven both methods to be more accurate than techniques usually utilized. (author)

  9. Innovative Research Methods for Rethinking Gender in CSPs

    DEFF Research Database (Denmark)

    McCarthy, Lauren

    2016-01-01

    Gender and women’s empowerment has become a big issue within CSPs in recent years. (See pages 58-61). Yet designing research projects that have proven impact on improving the lives of women in supply chains can be challenging, especially in CSPs where under - standings of ‘gender’ and ‘equality’ ......’ can differ culturally. This article therefore explores innovative research methods suitable to get a clearer view on gender issues.......Gender and women’s empowerment has become a big issue within CSPs in recent years. (See pages 58-61). Yet designing research projects that have proven impact on improving the lives of women in supply chains can be challenging, especially in CSPs where under - standings of ‘gender’ and ‘equality...

  10. A method for the isolation and characterization of functional murine monoclonal antibodies by single B cell cloning.

    Science.gov (United States)

    Carbonetti, Sara; Oliver, Brian G; Vigdorovich, Vladimir; Dambrauskas, Nicholas; Sack, Brandon; Bergl, Emilee; Kappe, Stefan H I; Sather, D Noah

    2017-09-01

    Monoclonal antibody technologies have enabled dramatic advances in immunology, the study of infectious disease, and modern medicine over the past 40years. However, many monoclonal antibody discovery procedures are labor- and time-intensive, low efficiency, and expensive. Here we describe an optimized mAb discovery platform for the rapid and efficient isolation, cloning and characterization of monoclonal antibodies in murine systems. In this platform, antigen-binding splenic B cells from immunized mice are isolated by FACS and cocultured with CD40L positive cells to induce proliferation and mAb production. After 12days of coculture, cell culture supernatants are screened for antigen, and IgG positivity and RNA is isolated for reverse-transcription. Positive-well cDNA is then amplified by PCR and the resulting amplicons can be cloned into ligation-independent expression vectors, which are then used directly to transfect HEK293 cells for recombinant antibody production. After 4days of growth, conditioned medium can be screened using biolayer interferometry for antigen binding and affinity measurements. Using this method, we were able to isolate six unique, functional monoclonal antibodies against an antigen of the human malaria parasite Plasmodium falciparum. Importantly, this method incorporates several important advances that circumvent the need for single-cell PCR, restriction cloning, and large scale protein production, and can be applied to a wide array of protein antigens. Copyright © 2017 Elsevier B.V. All rights reserved.

  11. Regional collaborations and indigenous innovation capabilities in China: A multivariate method for the analysis of regional innovation systems

    OpenAIRE

    Zhao, S.L.; Cacciolatti, L.; Lee, Soo Hee; Song, W.

    2014-01-01

    In this study we analyse the emerging patterns of regional collaboration for innovation projects in China, using official government statistics of 30 Chinese regions. We propose the use of Ordinal Multidimensional Scaling and Cluster analysis as a robust method to study regional innovation systems. Our results show that regional collaborations amongst organisations can be categorised by means of eight dimensions: public versus private organisational mindset; public versus private resources; i...

  12. Innovative Public Procurement Methods: Examples Of Selected Country And Lessons For Turkey

    Directory of Open Access Journals (Sweden)

    Elif Ayşe ŞAHİN İPEK

    2016-12-01

    Full Text Available Innovative public procurement considered as demand-side policies aimed at economic competitiveness, growth and development through the development of private sector innovation supply. In this study it is examined the methods of innovative procurement policy and country examples. It is exerted obstacles and solutions from the results of this examination.

  13. Governing Methods: Policy Innovation Labs, Design and Data Science in the Digital Governance of Education

    Science.gov (United States)

    Williamson, Ben

    2015-01-01

    Policy innovation labs are emerging knowledge actors and technical experts in the governing of education. The article offers a historical and conceptual account of the organisational form of the policy innovation lab. Policy innovation labs are characterised by specific methods and techniques of design, data science, and digitisation in public…

  14. Stimulating Creativity Methods And Innovative Performance In European Countries

    Directory of Open Access Journals (Sweden)

    Şipoş Gabriela Lucia

    2015-06-01

    Full Text Available A widely debated topic during the last decades focuses on the companies’ opportunities to acquire corporate competitiveness due to research, innovation and development. Thus, in the context of increased competition and current global challenges, fostering creativity and innovation is a way to boost economic growth and welfare of European countries. New and original ideas, skills, competencies and innovations they all could enable to achieve competitive advantages. Creative ideas and innovative solutions are crucial for the European countries in order to overcome the current economic crisis.

  15. Innovation

    African Journals Online (AJOL)

    The purpose of Innovation journal of appropriate librarianship and information work in Southern Africa is to publish material on libraries, information supply and other related matters in South and Southern Africa. Vol 45 (2012). DOWNLOAD FULL TEXT Open Access DOWNLOAD FULL TEXT Subscription or Fee Access ...

  16. Innovation

    DEFF Research Database (Denmark)

    Torfing, Jacob; Ricard, Lykke Margot

    2017-01-01

    Innovation i krydsfeltet mellem forskellige styringsparadigmer i offentlige organisationer. New Public Governance gør det muligt at skabe offentlig værdi på nye måder. Men NPG er ingen trylledrik, der fra den ene dag til den anden skaber balance mellem borgernes store forventninger og en trængt ø...

  17. Improving Machining Accuracy of CNC Machines with Innovative Design Methods

    Science.gov (United States)

    Yemelyanov, N. V.; Yemelyanova, I. V.; Zubenko, V. L.

    2018-03-01

    The article considers achieving the machining accuracy of CNC machines by applying innovative methods in modelling and design of machining systems, drives and machine processes. The topological method of analysis involves visualizing the system as matrices of block graphs with a varying degree of detail between the upper and lower hierarchy levels. This approach combines the advantages of graph theory and the efficiency of decomposition methods, it also has visual clarity, which is inherent in both topological models and structural matrices, as well as the resiliency of linear algebra as part of the matrix-based research. The focus of the study is on the design of automated machine workstations, systems, machines and units, which can be broken into interrelated parts and presented as algebraic, topological and set-theoretical models. Every model can be transformed into a model of another type, and, as a result, can be interpreted as a system of linear and non-linear equations which solutions determine the system parameters. This paper analyses the dynamic parameters of the 1716PF4 machine at the stages of design and exploitation. Having researched the impact of the system dynamics on the component quality, the authors have developed a range of practical recommendations which have enabled one to reduce considerably the amplitude of relative motion, exclude some resonance zones within the spindle speed range of 0...6000 min-1 and improve machining accuracy.

  18. Function combined method for design innovation of children's bike

    Science.gov (United States)

    Wu, Xiaoli; Qiu, Tingting; Chen, Huijuan

    2013-03-01

    As children mature, bike products for children in the market develop at the same time, and the conditions are frequently updated. Certain problems occur when using a bike, such as cycle overlapping, repeating function, and short life cycle, which go against the principles of energy conservation and the environmental protection intensive design concept. In this paper, a rational multi-function method of design through functional superposition, transformation, and technical implementation is proposed. An organic combination of frog-style scooter and children's tricycle is developed using the multi-function method. From the ergonomic perspective, the paper elaborates on the body size of children aged 5 to 12 and effectively extracts data for a multi-function children's bike, which can be used for gliding and riding. By inverting the body, parts can be interchanged between the handles and the pedals of the bike. Finally, the paper provides a detailed analysis of the components and structural design, body material, and processing technology of the bike. The study of Industrial Product Innovation Design provides an effective design method to solve the bicycle problems, extends the function problems, improves the product market situation, and enhances the energy saving feature while implementing intensive product development effectively at the same time.

  19. HOW DO FIRMS SOURCE EXTERNAL KNOWLEDGE FOR INNOVATION? ANALYSING EFFECTS OF DIFFERENT KNOWLEDGE SOURCING METHODS

    OpenAIRE

    KI H. KANG; JINA KANG

    2009-01-01

    In the era of "open innovation", external knowledge is a very important source for technology innovation. In this paper, we investigate the relationship between external knowledge and performance of technology innovation. The effect of external knowledge on the performance of technology innovation can vary with different external knowledge sourcing methods. We identify three ways of external knowledge sourcing: information transfer from informal network, R&D collaboration and technology acqui...

  20. Reassembling the Information Technology Innovation Process: An Actor Network Theory Method for Managing the Initiation, Production, and Diffusion of Innovations

    Science.gov (United States)

    Zendejas, Gerardo; Chiasson, Mike

    This paper will propose and explore a method to enhance focal actors' abilities to enroll and control the many social and technical components interacting during the initiation, production, and diffusion of innovations. The reassembling and stabilizing of such components is the challenging goal of the focal actors involved in these processes. To address this possibility, a healthcare project involving the initiation, production, and diffusion of an IT-based innovation will be influenced by the researcher, using concepts from actor network theory (ANT), within an action research methodology (ARM). The experiences using this method, and the nature of enrolment and translation during its use, will highlight if and how ANT can provide a problem-solving method to help assemble the social and technical actants involved in the diffusion of an innovation. Finally, the paper will discuss the challenges and benefits of implementing such methods to attain widespread diffusion.

  1. Supplier selection in manufacturing innovation chain-oriented public procurement based on improved PSO method

    Directory of Open Access Journals (Sweden)

    Xin Xu

    2014-01-01

    Full Text Available Purpose: At the dynamic innovation market, it is very difficult for an enterprise to accomplish innovation individually; technology innovation is shifting towards collaborative R&D chain mode. Thus, supplier selection based on individually innovation efficiency of enterprise is inapplicable to construct collaborative R&D innovation chain. This study is seeking to address how to select R&D innovation chain supplier in manufacturing industry. Design/methodology/approach: Firstly, Delphi method and AHP method are applied to establish an index system evaluating the suppliers of innovation chain, and then each index is weighted by experts with AHP method. Thirdly, optimized PSO algorithm is put forwarded based on the optimal efficiency of innovation chain to discriminate ideal suppliers meeting realistic conditions. Fourthly, innovation chain construction at generator manufacturing industry was taken as empirical case study to testify the improved PSO model. Findings: The innovation chain is comprised up by several enterprises, innovation performance of a single enterprise is not always positively correlated to that of one innovation chain, and the proposed model is capable to find out the best combination to construct an innovation chain. Research limitations/implications: The relations between these constructs with other variables of interest to the academicals fields were analyzed by a precise and credible data with a clear and concise description of the supply chain integration measurement scales. Practical implications: providing scales that are valid as a diagnostic tool for best practices, as well as providing a benchmark with which to compare the score for each individual plant against a chain of industrial innovation from machinery. Originality/value: Innovation chain integration is an important factor in explaining the innovation performance of companies. The vast range of results obtained is due to the fact that there is no exactness to

  2. Innovation

    OpenAIRE

    2012-01-01

    Présenté par FutuRIS, plate-forme prospective sur la recherche, l’innovation et la société animée par l’Association Nationale de la Recherche et de la Technologie, ce volume livre un panorama du système français de recherche et d’innovation dans son environnement européen. Sont abordés dans une première partie les champs décisionnels concernés, les politiques nationales menées en matière de R&D, les relations entre enseignement supérieur et recherche et l’Espace européen de la recherche à l’h...

  3. INNOVATION

    DEFF Research Database (Denmark)

    Helms, Niels Henrik

    2012-01-01

    Kravet om innovation og kreativitet er på flere måder en stor og en ny udfordring for voksenuddannelserne. Det udfordrer det didaktiske dilemma, det at vi skal gøres til kompetente og frie mennesker gennem pædagogiske handlinger, som netop pålægger os en ufrihed. – Men hvor denne ufrihed tidligere...... kunne begrundes med, at skolen eller uddannelsen vidste bedre, så er det ikke længere tilfældet. Skolen skal sørge for, at vi lærer noget – og ikke noget andet. Men det kan ikke længere med bestemthed afgøres, hvad det er vi skal lære i skolen, fordi det nye, det kreative og ikke mindst innovative...

  4. INNOVATIVE METHODS OF TEACHING HISTORY AT MODERN UNIVERSITIES

    Directory of Open Access Journals (Sweden)

    A. Yu. Suslov

    2017-01-01

    Full Text Available Introduction. As a discipline, History holds a specific place among disciplines of a humanitarian cycle of educational programs of higher education institutions regardless of university specialities. History plays an important role in the course of formation of a citizen and development of critical thinking of a personality as an element of a common culture. However, new federal standards require a drastic reduction of the classroom hours for studying a History course by students of non-humanitarian specialties, and, at the same time, enhancement of the contents of a discipline (its reorientation from History of Russia towards World History. Therefore, History programmes and courses demand up-to-date approaches, methods and didactic means to provide formation of holistic worldview of future experts.The aim of the article is to consider the features of innovative methods application in teaching history in high school taking into consideration modernization processes.Methodology and research methods. The research undertaken is based on activity and competence-based approaches. The methods of analysis and synthesis of the academic literature on the research topic were used; the methods of reflection and generalization of teaching activities of the Department of Humanitarian Disciplines of theKazanNationalResearchTechnologicalUniversity were applied as well.Results and scientific novelty. A modern view on historical education has been proposed as means of students’ systems thinking formation, designing the ideas about the world historical process among students, the mission ofRussia in this process, and evolution ofRussia as a part of the modern civilization. It is stated that History university course is designed not only to give the students strong subject knowledge, but also to create axiological orientations and abilities on the basis of the analysis of historical collisions, objective and subjective factors of society development. Moreover

  5. IMPROVED METHODS FOR EVALUATING THE EFFECTIVENESS OF INNOVATION DEVELOPMENT IN RUSSIAN CORPORATIONS

    Directory of Open Access Journals (Sweden)

    E. L. Dorzhieva

    2016-01-01

    Full Text Available Objectives. Innovative activity is a key factor in the effective development and growth of competitiveness in the Russian economy. An important role in this process is played by industrial corporations. Against this background, there is an increased need for improving the effectiveness of methods for evaluating the innovation development of Russian corporations. Methods. Formal logic as well as system analysis methods were used in the research, allowing us to consider the corporation as a system that includes a variety of innovational directions (elements. Results. The article discusses various approaches to the determination of the basic definitions of innovation; a classification of innovation is proposed. The attributes of innovative activity of industrial corporations are distinguished together with an outline of the possible causes of various innovative corporate activities; components of the system of indicators of innovation activity are isolated: financial; consumer; process; development and training; risk management. The need for the indices of the innovation activity of the risk component to be included in the composition of the system is substantiated. It is shown how the objectives for each area of innovation may be achieved in tandem with a methodological approach that allows continuous monitoring of the implementation of innovative development strategies. An algorithm for evaluating the implementation of innovative strategies contributing to the development of industrial corporations is presented. Conclusion. Theoretical and methodological development can be used not only to navigate the variety of innovations but also to determine and establish the relationship and interdependence between the various innovations as well as carry out analysis, assessment and forecasting for the effective development of innovative activity of Russian corporations. 

  6. Methods to Implement Innovation and Entrepreneurship in Physics

    Science.gov (United States)

    Arion, Douglas

    2015-03-01

    The physics community is beginning to become aware of the benefits of entrepreneurship and innovation education: greater enrollments, improved students satisfaction, a wider range of interesting research problems, and the potential for greater return from more successful alumni. This talk will suggest a variety of mechanisms by which physics departments can include entrepreneurship and innovation content within their programs - without necessarily requiring earth-shattering changes to the curriculum. These approaches will thus make it possible for departments to get involved with entrepreneurship and innovation, and grow those components into vibrant activities for students and faculty.

  7. INNOVATIVE USE OF QUALITY MANAGEMENT METHODS FOR PRODUCT IMPROVEMENT

    Directory of Open Access Journals (Sweden)

    Katarzyna MIDOR

    2016-10-01

    Full Text Available Organisations constantly look for new, innovative solutions and methods which could be used to improve their efficiency and increase the quality of their products. Identifying the causes for returns is an important issue for modern compa-nies, as returns are the cause for the increase in production costs and, most importantly, the loss of credibility in the eyes of the client. Therefore, for the company to be able to sustain or strengthen its position on the market, it has to follow the rules of quality management. Especially important is the rule of constant improvement. This rule is primarily connected with preventing errors and defects from occurring at all the stages of the production process. To achieve that, one must, among other things, use quality management tools. The article presents an analysis of causes for returns of a vibrating screen produced by a company which manufactures machinery and equipment for the extractive industry, using quality management tools such as the Ishikawa diagram and Pareto analysis. The analysis allowed for the identifi-cation of the causes of client returns which could not be previously identified, and proposing solutions for them.

  8. Innovative Use of Quality Management Methods for Product Improvement

    Science.gov (United States)

    Midor, Katarzyna; Žarnovský, Jozef

    2016-12-01

    Organisations constantly look for new, innovative solutions and methods which could be used to improve their efficiency and increase the quality of their products. Identifying the causes for returns is an important issue for modern companies, as returns are the cause for the increase in production costs and, most importantly, the loss of credibility in the eyes of the client. Therefore, for the company to be able to sustain or strengthen its position on the market, it has to follow the rules of quality management. Especially important is the rule of constant improvement. This rule is primarily connected with preventing errors and defects from occurring at all the stages of the production process. To achieve that, one must, among other things, use quality management tools. The article presents an analysis of causes for returns of a vibrating screen produced by a company which manufactures machinery and equipment for the extractive industry, using quality management tools such as the Ishikawa diagram and Pareto analysis. The analysis allowed for the identification of the causes of client returns which could not be previously identified, and proposing solutions for them.

  9. WasteWise Resource Management: Innovative Solid Waste Contracting Methods

    Science.gov (United States)

    Resource management is an innovative contractual partnership between a waste-generating organization and a qualified contractor that changes the nature of current disposal services to support waste minimization and recycling.

  10. Using of innovative educational and traning methods in the professional training of social workers

    Directory of Open Access Journals (Sweden)

    Lidiia Tymkiv

    2017-03-01

    Full Text Available The article deals with the use of innovative methods of training professional values ofsocial workers. It has been shown, that the use of innovative technologies in the educationalprocess will enhance the mental and social activities of prospective specialists in the socialsphere and prepare them for independent decision-making consistent with the acquired values.Key words : innovative methods of training, role games, game situation of professionaldirection.

  11. Isolation of full-length putative rat lysophospholipase cDNA using improved methods for mRNA isolation and cDNA cloning

    International Nuclear Information System (INIS)

    Han, J.H.; Stratowa, C.; Rutter, W.J.

    1987-01-01

    The authors have cloned a full-length putative rat pancreatic lysophospholipase cDNA by an improved mRNA isolation method and cDNA cloning strategy using [ 32 P]-labelled nucleotides. These new methods allow the construction of a cDNA library from the adult rat pancreas in which the majority of recombinant clones contained complete sequences for the corresponding mRNAs. A previously recognized but unidentified long and relatively rare cDNA clone containing the entire sequence from the cap site at the 5' end to the poly(A) tail at the 3' end of the mRNA was isolated by single-step screening of the library. The size, amino acid composition, and the activity of the protein expressed in heterologous cells strongly suggest this mRNA codes for lysophospholipase

  12. Identification of potential nuclear reprogramming and differentiation factors by a novel selection method for cloning chromatin-binding proteins

    International Nuclear Information System (INIS)

    Wang Liu; Zheng Aihua; Yi Ling; Xu Chongren; Ding Mingxiao; Deng Hongkui

    2004-01-01

    Nuclear reprogramming is critical for animal cloning and stem cell creation through nuclear transfer, which requires extensive remodeling of chromosomal architecture involving dramatic changes in chromatin-binding proteins. To understand the mechanism of nuclear reprogramming, it is critical to identify chromatin-binding factors specify the reprogramming process. In this report, we have developed a high-throughput selection method, based on T7 phage display and chromatin immunoprecipitation, to isolate chromatin-binding factors expressed in mouse embryonic stem cells using primary mouse embryonic fibroblast chromatin. Seven chromatin-binding proteins have been isolated by this method. We have also isolated several chromatin-binding proteins involved in hepatocyte differentiation. Our method provides a powerful tool to rapidly and selectively identify chromatin-binding proteins. The method can be used to study epigenetic modification of chromatin during nuclear reprogramming, cell differentiation, and transdifferentiation

  13. Forms and methods of stimulation of innovative activities in the restructuring of production program

    Directory of Open Access Journals (Sweden)

    I. I. Emtcova

    2016-01-01

    Full Text Available In the Russian economy not every business entity, implements innovative business activities. The situation generated by the complexity of perception and practical transition to an innovative economic system. On the development of innovative activities affects the overall condition of the economy, condition of material production. The research demonstrates that resource potential of innovative activities in recent years had a tendency towards absolute quantitative reduction and quality deterioration. The decrease in the level and quality of resource provision of innovative activity due to the lack of necessary financial resources. Currently, innovation has become the primary means of increasing the profit of economic entities at the expense of better meet market demand, reduce production costs compared to competitors. Given the complexity of businesses, there is a need of the state stimulation of innovative activity, which is carried out the main directions, forms and methods. In the system of direct effects of the state on business innovation is the stimulation of development of Technopark structures. Creating the most favourable conditions for innovative enterprises, the provision of various services is their main goal. For the food processing industry currently, the largest share in the investments in the investment activities have their own sources of funding, including the use of depreciation. To Finance industry-wide, cross-sectoral and regional scientific and technical problems you can create extra-budgetary funds for financing R & d and innovation support. To encourage regional interests, one of which is that innovation is available to local authorities. In the financial provision of innovative activity is given credit. A Bank loan allows you to increase the efficiency of innovation activity. The article concludes that these measures to stimulate innovative-innovative activity can effectively influence the activity of the company: will

  14. EasyClone: method for iterative chromosomal integration of multiple genes in Saccharomyces cerevisiae

    DEFF Research Database (Denmark)

    Jensen, Niels Bjerg; Strucko, Tomas; Kildegaard, Kanchana Rueksomtawin

    2014-01-01

    of multiple genes with an option of recycling selection markers. The vectors combine the advantage of efficient uracil excision reaction-based cloning and Cre-LoxP-mediated marker recycling system. The episomal and integrative vector sets were tested by inserting genes encoding cyan, yellow, and red...... fluorescent proteins into separate vectors and analyzing for co-expression of proteins by flow cytometry. Cells expressing genes encoding for the three fluorescent proteins from three integrations exhibited a much higher level of simultaneous expression than cells producing fluorescent proteins encoded...... on episomal plasmids, where correspondingly 95% and 6% of the cells were within a fluorescence interval of Log10 mean ± 15% for all three colors. We demonstrate that selective markers can be simultaneously removed using Cre-mediated recombination and all the integrated heterologous genes remain...

  15. Innovative methods to reduce salt water intrusion in harbours

    Science.gov (United States)

    Groenenboom, J.; Uittenbogaard, R.; Hulsen, L.; van der Kaaij, T.; Kielen, N.

    2017-12-01

    The availability of fresh water in densely populated estuarine environments will in the future more often be threatened due to both human (e.g. channel deepening) and natural (sea-level rise, storm surges, extremely low river discharges) causes. Here, the salt water intrusion into the New Waterway, the main navigation channel of the port of Rotterdam, is used as a case study to elaborate on two innovative ways to mitigate the effects of salt water intrusion. The first method is based on the concept that vertical mixing of a salt wedge reduces its intrusion length. The idea is to equip a vessel with cranes that hold perforated tubes close to the bed alongside the vessel. By connecting compressors to the perforated tubes, a bubble screen with an adjustable vertical location can be created. Since the horizontal location of the bubble screens is not fixed, the vessel can sail in the vicinity of the moving salt wedge therewith increasing the effectiveness of the method. Another advantage of this intervention is that it can be deployed temporarily when the urgency for the prevention of salt water intrusion is high. The second method originates from the Port of Rotterdam Authority and is inspired by a small bypass that is present between two parallel channels (New Waterway and Caland Canal) connecting the North Sea to the Port of Rotterdam. Due to the different hydrodynamic characteristics of the hinterland of both channels, a difference in salinity and water level is present between both ends of the bypass. As a result, a lateral inflow of water into the New Waterway occurs at the same moment that the flood velocities transport saline water landwards. The lateral inflow of water into this channel has no momentum in the landward direction and therefore decreases the landward flow velocity and therewith the salt water intrusion. In addition, the inflow drives a vertical circulation that mixes the water column close to the bypass. Similar to the bubble screens mentioned

  16. Academic Cloning.

    Science.gov (United States)

    Sikula, John P.; Sikula, Andrew F.

    1980-01-01

    The authors define "cloning" as an integral feature of all educational systems, citing teaching practices which reward students for closely reproducing the teacher's thoughts and/or behaviors and administrative systems which tend to promote like-minded subordinates. They insist, however, that "academic cloning" is not a totally…

  17. THE RISKS’ ASSESSMENT IN INNOVATIVE PROJECTS BY THE METHOD OF VERIFIED EQUIVALENTS

    Directory of Open Access Journals (Sweden)

    Анатолій Валентинович ШАХОВ

    2017-03-01

    Full Text Available The article describes the concept of "risk of innovation", identified the causes of the risk and the methods of eliminating of negative manifestations of the risk situations in innovative projects. The advantages and disadvantages of the method of correction of the discount rate and the method of equivalent annuities are considered. The methodical approach in assessing the expected effect of the innovative project based on the concept of probability-interval uncertainty is proposed in the article. It was established that the analyzed approaches can be used for the accounting of the risk of innovative projects. Project manager makes his choice using any method of risk assessment individually, depending on the extent and characteristics of the project, the degree of novelty and scale introduction of innovative products, the number of participants and the level of requirements of the foundation of project efficiency and other factors.

  18. Strategies for improving quality and methods for innovation services

    Directory of Open Access Journals (Sweden)

    Bajić Milan

    2009-01-01

    Full Text Available Contemporary concepts of business of service organisations, focus their researches on measuring quality of the services and identification of mistakes. The aim of strategies is the improvement of quality. Marketing concepts shows that the constant creation of new products and the modification of characteristics on the old products, makes possible market strategy that stimulates development of activities. This paper is focusing on the improving of service using the strategies that can innovate and improve the services. Paper seeks to demonstrate one of possible observations and importance of performance, combined with blueprinting of services and benchmarking as a useful strategies for improving and innovation of services.

  19. Recombinational Cloning Using Gateway and In-Fusion Cloning Schemes

    Science.gov (United States)

    Throop, Andrea L.; LaBaer, Joshua

    2015-01-01

    The comprehensive study of protein structure and function, or proteomics, depends on the obtainability of full-length cDNAs in species-specific expression vectors and subsequent functional analysis of the expressed protein. Recombinational cloning is a universal cloning technique based on site-specific recombination that is independent of the insert DNA sequence of interest, which differentiates this method from the classical restriction enzyme-based cloning methods. Recombinational cloning enables rapid and efficient parallel transfer of DNA inserts into multiple expression systems. This unit summarizes strategies for generating expression-ready clones using the most popular recombinational cloning technologies, including the commercially available Gateway® (Life Technologies) and In-Fusion® (Clontech) cloning technologies. PMID:25827088

  20. Sequential method for the assessment of innovations in computer assisted industrial processes

    International Nuclear Information System (INIS)

    Suarez Antola R.

    1995-01-01

    A sequential method for the assessment of innovations in industrial processes is proposed, using suitable combinations of mathematical modelling and numerical simulation of dynamics. Some advantages and limitations of the proposed method are discussed. tabs

  1. A Polymerase Chain Reaction-Based Method for Isolating Clones from a Complimentary DNA Library in Sheep

    Science.gov (United States)

    Friis, Thor Einar; Stephenson, Sally; Xiao, Yin; Whitehead, Jon

    2014-01-01

    The sheep (Ovis aries) is favored by many musculoskeletal tissue engineering groups as a large animal model because of its docile temperament and ease of husbandry. The size and weight of sheep are comparable to humans, which allows for the use of implants and fixation devices used in human clinical practice. The construction of a complimentary DNA (cDNA) library can capture the expression of genes in both a tissue- and time-specific manner. cDNA libraries have been a consistent source of gene discovery ever since the technology became commonplace more than three decades ago. Here, we describe the construction of a cDNA library using cells derived from sheep bones based on the pBluescript cDNA kit. Thirty clones were picked at random and sequenced. This led to the identification of a novel gene, C12orf29, which our initial experiments indicate is involved in skeletal biology. We also describe a polymerase chain reaction-based cDNA clone isolation method that allows the isolation of genes of interest from a cDNA library pool. The techniques outlined here can be applied in-house by smaller tissue engineering groups to generate tools for biomolecular research for large preclinical animal studies and highlights the power of standard cDNA library protocols to uncover novel genes. PMID:24447069

  2. Determination of Innovation Capability of Organizations: Qualitative Meta Synthesis and Delphi Method

    DEFF Research Database (Denmark)

    Momeni, Mostafa; Nielsen, Susanne Balslev; Kafash, Mahdi Haghighi

    2015-01-01

    . The innovation capability is associated with other organizational capabilities. So, many organizations have focused on the need to identify innovation capabilities and resources or strengths in relation to external opportunities and threats according to inside-out view because innovation capability has...... capability in the organizations and this paper includes an expert based validation in three rounds of the Delphi method. This research proposed a direct relationship between Innovation Capability and three main capabilities that is called Structural Capability, Personnel Capability and Operational Capability...

  3. APPLICATION OF FORMS AND METHODS OF COMMERCIALIZATION OF INNOVATIONS THROUGH THE INTERACTION OF UNIVERSITIES AND ENTERPRISES

    Directory of Open Access Journals (Sweden)

    Svetlana E. Sitnikova

    2018-03-01

    Full Text Available In the conditions of formation of economy based on knowledge, a key condition for successful development is the effective integration of science, education and business. Currently, the commercialization of innovation becomes a necessary factor for sustainable development of universities, one of their main tools to increase its competitiveness in the market of educational services and products. The efficiency of the process of innovation commercialization is conditioned by the rational choice of forms and methods of implementing this process. The interaction of universities and enterprises in the innovative clusters provides benefits such as effective dissemination of information about the creation of innovations, the relevant areas of research, the granting of preferences to cluster participants. The creation of technoparks suggests the presence of a specially allocated site, which houses many new businesses, so that existing institutions can only be partners of the technopark. From the point of view of commercialization of University innovation, the cluster seems to be a more attractive method compared with the creation of technology parks, but its application is necessary to adjust the approach to conducting the state policy in the field of innovation management. The interaction of universities and enterprises via an intermediary – the chamber of commerce and industry – can be described as an obstacle to the commercialization of university innovations. On the contrary, technological innovation center protects the interests of universities, rather than enterprises, as it is created to facilitate the commercialization of university innovation. Direct interaction of universities and enterprises becomes possible with the help of methods of a single transaction, a regular cooperation and a contract for the supply of innovation. The choice of methods of commercialization of innovations should be made on the basis of existing university

  4. Methods for Practising Ethics in Research and Innovation : A Literature Review, Critical Analysis and Recommendations

    NARCIS (Netherlands)

    Reijers, Wessel; Wright, David; Brey, Philip; Weber, Karsten; Rodrigues, Rowena; O’Sullivan, Declan; Gordijn, Bert

    2017-01-01

    This paper provides a systematic literature review, analysis and discussion of methods that are proposed to practise ethics in research and innovation (R&I). Ethical considerations concerning the impacts of R&I are increasingly important, due to the quickening pace of technological innovation and

  5. Utilizing the Lead User Method for Promoting Innovation in E-Recuiting

    NARCIS (Netherlands)

    Furtmueller-Ettinger, Elfriede; Wilderom, Celeste P.M.; van Dick, Rolf; Bondarouk, Tatiana; Ruel, Hubertus Johannes Maria; Guiderdoni-Jourdain, Karine; Oiry, Ewan

    2009-01-01

    In order to maintain their customer base, many e-recruiting firms are in need of developing innovations. The Lead User (LU) Method has been heralded in the new product innovation literature but not yet applied often in e-service settings. Based on an e-recruiting portal, the authors compare new

  6. Why Clone?

    Science.gov (United States)

    ... than expected. Could we really clone dinosaurs? In theory? Yes. You would need: A well-preserved source ... it raises a number of ethical, legal, and social challenges that need to be considered. The vast ...

  7. Human Cloning

    National Research Council Canada - National Science Library

    Johnson, Judith A; Williams, Erin D

    2006-01-01

    .... Scientists in other labs, including Harvard University and the University of California at San Francisco, intend to produce cloned human embryos in order to derive stem cells for medical research...

  8. Innovations

    Science.gov (United States)

    Estee, Charles R., Ed.

    1973-01-01

    Introduces packaged learning modules in environmental and applied sciences education, general-education courses with emphases on chemistry impact on various institutions, methods of presenting homework solutions by tape cassettes, and experimental approaches to teaching of science for inservice elementary teachers in Vancouver, Canada. (CC)

  9. Interdisciplinary Approaches and Methods for Sustainable Transformation and Innovation

    Directory of Open Access Journals (Sweden)

    Sangkyun Kim

    2015-04-01

    Full Text Available To increase the likelihood of success and sustainability, organizations must fundamentally reposition themselves and try to change current processes or create new products and services. One of the most effective approaches to find a solution for transformation and innovation is to learn from other domains where a solution for similar problems is already available. This paper briefly introduces the definition of and approaches to convergence of academic disciplines and industries, and overviews several representative convergence cases focusing on gamification for sustainable education, environments, and business managements.

  10. Impacts of an Innovative Residential Construction Method on Internal Conditions

    Directory of Open Access Journals (Sweden)

    Roger Birchmore

    2015-02-01

    Full Text Available New Zealand houses are known for producing sub-optimal internal thermal conditions and unacceptably high internal moisture levels. These contribute to poor levels of health, mould and can coincide with the decay of structural timber frames. A proposed solution is to provide an alternative structure utilising plywood, a vapour check on the internal face of the timber frame and an additional air gap, followed by the internal lining. The internal vapour check is designed to prevent moisture vapour diffusion from inside into the frame and to permit moisture diffusion from outside through the structure to the internal environment. Two full scale houses had temperatures, dew points and humidity levels monitored in passive, unoccupied conditions. The test case house incorporated the innovative construction solution. The control house was of identical design and location, using standard construction practice. The calculated internal moisture content profile appeared to be unrelated to the external moisture content as expected, instead following the profile of the changing internal temperature. Whilst the innovative construction appeared to prevent moisture diffusion into the structure in winter and permit it inside in summer, this resulted in a generally higher internal relative humidity than the control house.

  11. Estimating the direction of innovative change based on theory an mixed methods

    NARCIS (Netherlands)

    Geurts, Petrus A.T.M.; Roosendaal, Hans E.

    2001-01-01

    In predicting the direction of innovative changethe question arises of the valid measurement ofyet unknown variables. We developed and applied aresearch method that combines qualitativeand quantitative elements in one interview formatand an analysis tool suitable for these data. Animportant

  12. Development of risk benefit structural design method for innovative reactor plants

    International Nuclear Information System (INIS)

    Yoshio Kamishima; Tai Asayama; Yukio Takahashi; Masanori Tashimo; Hideo Machida; Yomomi Otani; Yasuharu Chuman

    2005-01-01

    The development of innovative nuclear plants where the energy in the future is carried out in Japan. The design method based on a risk benefit of having maintained mitigation of a risk and the improvement in economy is called for, in order to realize the national innovative nuclear plants. Main key technologies of the risk benefit structural design method are crack propagation evaluation technology and structural reliability evaluation technology. This research aims at pulling up these two technologies on an engineering practical use level. In this paper, requirements from the design of typical innovative nuclear plants and research plan are shown.(authors)

  13. Cloning of transgenic tobacco BY-2 cells; an efficient method to analyse and reduce high natural heterogeneity of transgene expression.

    Science.gov (United States)

    Nocarova, Eva; Fischer, Lukas

    2009-04-22

    Phenotypic characterization of transgenic cell lines, frequently used in plant biology studies, is complicated because transgene expression in individual cells is often heterogeneous and unstable. To identify the sources and to reduce this heterogeneity, we transformed tobacco (Nicotiana tabacum L.) BY-2 cells with a gene encoding green fluorescent protein (GFP) using Agrobacterium tumefaciens, and then introduced a simple cloning procedure to generate cell lines derived from the individual transformed cells. Expression of the transgene was monitored by analysing GFP fluorescence in the cloned lines and also in lines obtained directly after transformation. The majority ( approximately 90%) of suspension culture lines derived from calli that were obtained directly from transformation consisted of cells with various levels of GFP fluorescence. In contrast, nearly 50% of lines generated by cloning cells from the primary heterogeneous suspensions consisted of cells with homogenous GFP fluorescence. The rest of the lines exhibited "permanent heterogeneity" that could not be resolved by cloning. The extent of fluorescence heterogeneity often varied, even among genetically identical clones derived from the primary transformed lines. In contrast, the offspring of subsequent cloning of the cloned lines was uniform, showing GFP fluorescence intensity and heterogeneity that corresponded to the original clone. The results demonstrate that, besides genetic heterogeneity detected in some lines, the primary lines often contained a mixture of epigenetically different cells that could be separated by cloning. This indicates that a single integration event frequently results in various heritable expression patterns, which are probably accidental and become stabilized in the offspring of the primary transformed cells early after the integration event. Because heterogeneity in transgene expression has proven to be a serious problem, it is highly advisable to use transgenes tagged with

  14. INNOVATIVE METHODS IN THE DEVELOPMENT OF RURAL TOURISM IN ROMANIA

    Directory of Open Access Journals (Sweden)

    Avram Daniel

    2015-05-01

    Full Text Available Innovations are considered the engine of economic growth, because they serve a s basis for obtaining the competitive advantage. Tourism is one of the most profitable and dynamic sectors of economy, occupying the second position in the international trade after oil. Rural tourism also has major implications in the economic, social and cultural development of villages. This study presents an analysis of the fluctuation of the number of employees and of the number of accommodation units specific to rural tourism in Romania, between 2007-2014. To this end statistical data from the National Statistics Institute has been used. Volunteer tourism, the development of national portals for presentation of vacant jobs in the tourism sector and the development of human resources by absorption of European funds, are the three suggestions presented in this study, which have the purpose ofreinvigorating rural tourism in Romania.

  15. DNA microarrays : a molecular cloning manual

    National Research Council Canada - National Science Library

    Sambrook, Joseph; Bowtell, David

    2002-01-01

    .... This manual, designed to extend and to complement the information in the best-selling Molecular Cloning, is a synthesis of the expertise and experience of more than 30 contributors all innovators in a fast moving field...

  16. Effect of roscovitine treated donor cells and different activation methods on development of handmade cloned goat (Capra hircus) embryos.

    Science.gov (United States)

    Akshey, Y S; Malakar, D; De, A Kumar; Jena, M Kumar; Pawar, S Kumar; Dutta, R; Sahu, S

    2011-05-01

    The aim of the present investigation was to find out the effects of roscovitine treatment of donor cells and different activation methods on development of HMC goat embryos. Goat fetal fibroblast cells were cultured and divided into three treatment groups-contact inhibition group, roscovitine treatment group and serum starvation group. There was a significant decrease in blastocyst yield in serum starvation group (6.82%) compared to roscovitine treatment group (19.31%) and contact inhibition group (18.52%), however, no significant difference was found between roscovitine treatment group and contact inhibition group. To see the effect of different methods of activation, the reconstructed embryos were randomly divided into two groups and activated by two methods-one half by 2 μM Ca ionophore and another half by 2.31 kV/cm for 15 μSec electrical pulse. Subsequently, cloned embryos were cultured in TCM-199 based embryo development medium supplemented with 10 mg/mL bovine serum albumin in WOW culture system. There was a significant increase in the rate of cleavage and blastocyst production in electric pulse activation of 78.57% and 21.43% than Ca ionophore activation of 62.63% and 10.61% respectively. In conclusion, treatment of donor cells with roscovitine yields a significantly increased blastocyst than serum starved donor cells but equivalent blastocyst to contact inhibition group and electrical pulse activation (EPA) improves the production of HMC goat embryos. Copyright © 2011 Elsevier Inc. All rights reserved.

  17. Measurement of in vivo HGPRT-deficient mutant cell frequency using a modified method for cloning human peripheral blood T-lymphocytes

    International Nuclear Information System (INIS)

    Hakoda, Masayuki; Akiyama, Mitoshi; Kyoizumi, Seishi; Kobuke, Kyoko; Awa, A.A.

    1987-07-01

    Approximately 80 % of human peripheral blood T-lymphocytes could be cloned in the presence of crude Interleukin-2, phytohemagglutinin, and X-irradiated autologous lymphocytes and Raji B-cells. This modified cloning method was used to measure the in vivo frequency of HGPRT-deficient mutant T-lymphocytes. Repeated experiments using blood from the same individuals revealed that the frequency of mutant cells was almost constant for each individual even though the cloning efficiency of lymphocytes varied somewhat from experiment to experiment. Approximately 80 % of both wild-type unselected and 6-thioguanine-resistant colonies had helper/inducer and about 20 % had suppressor/cytotoxic T-lymphocyte markers. No difference was observed in the distribution of lymphocyte subsets between wild and mutant lymphocyte colonies. (author)

  18. The first report: An analysis of bacterial flora of the first voided urine specimens of patients with male urethritis using the 16S ribosomal RNA gene-based clone library method.

    Science.gov (United States)

    You, Chunlin; Hamasuna, Ryoichi; Ogawa, Midori; Fukuda, Kazumasa; Hachisuga, Toru; Matsumoto, Tetsuro; Taniguchi, Hatsumi

    2016-06-01

    To analyse the bacterial flora of urine from patients with male urethritis using the clone library method. Urine specimens from patients with urethritis were used. The bacterial flora was analysed according to the 16S ribosomal RNA gene-based clone library method. In addition, Neisseria gonorrhoeae, Chlamydia trachomatis, Mycoplasma genitalium, Mycoplasma hominis, Ureaplasma urealyticum or Ureaplasma parvum were detected by the conventional PCR methods (TMA or real-time PCR) and data from the clone library and conventional PCR methods were compared. Among 58 urine specimens, 38 were successfully analysed using the clone library method. From the specimens, 2427 clones were evaluated and 95 bacterial phylotypes were detected. N. gonorrhoeae was detected from 6 specimens and as the predominant bacterial species in 5 specimens. M. genitalium was detected from 5 specimens and as the predominant bacterial species in 3 specimens. C. trachomatis was detected from 15 specimens using the TMA method, but was detected from only 1 specimen using the clone library method. U. parvum was detected from only 2 specimens using the clone library method. In addition, Haemophilus influenzae and Neisseria meningitidis were also detected in 8 and 1 specimens, respectively. Gardnerella vaginalis, which is a potential pathogen for bacterial vaginitis in women, was detected in 10 specimens. The clone library method can detect the occupancy rate of each bacteria species among the bacterial flora and may be a new method for bacterial analyses in male urethritis. Copyright © 2016 Elsevier Ltd. All rights reserved.

  19. Transforming Elementary Science Teacher Education by Bridging Formal and Informal Science Education in an Innovative Science Methods Course

    Science.gov (United States)

    Riedinger, Kelly; Marbach-Ad, Gili; McGinnis, J. Randy; Hestness, Emily; Pease, Rebecca

    2011-01-01

    We investigated curricular and pedagogical innovations in an undergraduate science methods course for elementary education majors at the University of Maryland. The goals of the innovative elementary science methods course included: improving students' attitudes toward and views of science and science teaching, to model innovative science teaching…

  20. Molecular Profiling of Microbial Communities from Contaminated Sources: Use of Subtractive Cloning Methods and rDNA Spacer Sequences; FINAL

    International Nuclear Information System (INIS)

    Robb, Frank T.

    2001-01-01

    The major objective of this research was to provide appropriate sequences and assemble a DNA array of oligonucleotides to be used for rapid profiling of microbial populations from polluted areas and other areas of interest. The sequences to be assigned to the DNA array were chosen from cloned genomic DNA taken from groundwater sites having well characterized pollutant histories at Hanford Nuclear Plant and Lawrence Livermore Site 300. Glass-slide arrays were made and tested; and a new multiplexed, bead-based method was developed that uses nucleic acid hybridization on the surface of microscopic polystyrene spheres to identify specific sequences in heterogeneous mixtures of DNA sequences. The test data revealed considerable strain variation between sample sites showing a striking distribution of sequences. It also suggests that diversity varies greatly with bioremediation, and that there are many bacterial intergenic spacer region sequences that can indicate its effects. The bead method exhibited superior sequence discrimination and has features for easier and more accurate measurement

  1. Genome-wide engineering of an infectious clone of herpes simplex virus type 1 using synthetic genomics assembly methods.

    Science.gov (United States)

    Oldfield, Lauren M; Grzesik, Peter; Voorhies, Alexander A; Alperovich, Nina; MacMath, Derek; Najera, Claudia D; Chandra, Diya Sabrina; Prasad, Sanjana; Noskov, Vladimir N; Montague, Michael G; Friedman, Robert M; Desai, Prashant J; Vashee, Sanjay

    2017-10-17

    Here, we present a transformational approach to genome engineering of herpes simplex virus type 1 (HSV-1), which has a large DNA genome, using synthetic genomics tools. We believe this method will enable more rapid and complex modifications of HSV-1 and other large DNA viruses than previous technologies, facilitating many useful applications. Yeast transformation-associated recombination was used to clone 11 fragments comprising the HSV-1 strain KOS 152 kb genome. Using overlapping sequences between the adjacent pieces, we assembled the fragments into a complete virus genome in yeast, transferred it into an Escherichia coli host, and reconstituted infectious virus following transfection into mammalian cells. The virus derived from this yeast-assembled genome, KOS YA , replicated with kinetics similar to wild-type virus. We demonstrated the utility of this modular assembly technology by making numerous modifications to a single gene, making changes to two genes at the same time and, finally, generating individual and combinatorial deletions to a set of five conserved genes that encode virion structural proteins. While the ability to perform genome-wide editing through assembly methods in large DNA virus genomes raises dual-use concerns, we believe the incremental risks are outweighed by potential benefits. These include enhanced functional studies, generation of oncolytic virus vectors, development of delivery platforms of genes for vaccines or therapy, as well as more rapid development of countermeasures against potential biothreats.

  2. Efficient recovery of environmental DNA for expression cloning by indirect extraction methods

    NARCIS (Netherlands)

    Gabor, Esther; de Vries, Erik; Janssen, DB

    2003-01-01

    Using direct and cell extraction-based (indirect) isolation methods, DNA was obtained from environmental samples with largely differing characteristics (loam soil, sand soil, sediment, activated sludge, and compost) and evaluated with respect to the comprised bacterial diversity and its suitability

  3. Student Characteristics, Prior Experiences, and the Perception of Mixed Methods as an Innovation

    Science.gov (United States)

    Brown, Sydney E.

    2014-01-01

    There are persistent challenges to teaching mixed methods and innovative solutions are sought in order to address the needs of an increasingly diverse global audience seeking mixed methods instruction. This mixed methods study was conducted to gain insights to course design by more fully understanding the relationships among graduate student…

  4. Room for improvement? Leadership, innovation culture and uptake of quality improvement methods in general practice.

    Science.gov (United States)

    Apekey, Tanefa A; McSorley, Gerry; Tilling, Michelle; Siriwardena, A Niroshan

    2011-04-01

    Leadership and innovation are currently seen as essential elements for the development and maintenance of high-quality care. Little is known about the relationship between leadership and culture of innovation and the extent to which quality improvement methods are used in general practice. This study aimed to assess the relationship between leadership behaviour, culture of innovation and adoption of quality improvement methods in general practice. Self-administered postal questionnaires were sent to general practitioner quality improvement leads in one county in the UK between June and December 2007. The questionnaire consisted of background information, a 12-item scale to assess leadership behaviour, a seven-dimension self-rating scale for culture of innovation and questions on current use of quality improvement tools and techniques. Sixty-three completed questionnaires (62%) were returned. Leadership behaviours were not commonly reported. Most practices reported a positive culture of innovation, featuring relationship most strongly, followed by targets and information but rated lower on other dimensions of rewards, risk and resources. There was a significant positive correlation between leadership behaviour and the culture of innovation (r = 0.57; P improvement methods were not adopted by most participating practices. Leadership behaviours were infrequently reported and this was associated with a limited culture of innovation in participating general practices. There was little use of quality improvement methods beyond clinical and significant event audit. Practices need support to enhance leadership skills, encourage innovation and develop quality improvement skills if improvements in health care are to accelerate. © 2010 Blackwell Publishing Ltd.

  5. A reliable combination method to identification and typing of epidemic and endemic clones among clinical isolates of Acinetobacter baumannii.

    Science.gov (United States)

    Piran, Arezoo; Shahcheraghi, Fereshteh; Solgi, Hamid; Rohani, Mahdi; Badmasti, Farzad

    2017-10-01

    The multi-drug resistant (MDR) Acinetobacter baumannii as an important nosocomial pathogen has emerged a global health concern in recent years. In this study, we applied three easier, faster, and cost-effective methods including PCR-based open reading frames (ORFs) typing, sequence typing of bla OXA-51-like and RAPD-PCR method to rapid typing of A. baumannii strains. Taken together in the present study the results of ORFs typing, PCR-sequencing of bla OXA-51-like genes and MLST sequence typing revealed there was a high prevalence (62%, 35/57) of ST2 as international and successful clone which detected among clinical isolates of multi-drug resistant A. baumannii with ORF pattern B and bla OXA-66 gene. Only 7% (4/57) of MDR isolates belonged to ST1 with ORF pattern A and bla OXA-69 gene. Interestingly, we detected singleton ST513 (32%, 18/57) that encoded bla OXA-90 and showed the ORF pattern H as previously isolated in Middle East. Moreover, our data showed RAPD-PCR method can detect divergent strains of the STs. The Cl-1, Cl-2, Cl-3, Cl-4, Cl-10, Cl-11, Cl-12, Cl-13 and Cl-14 belonged to ST2. While the Cl-6, Cl-7, Cl-8 and Cl-9 belonged to ST513. Only Cl-5 belonged to ST1. It seems that the combination of these methods have more discriminatory than any method separately and could be effectively applied to rapid detection of the clonal complex (CC) of A. baumannii strains without performing of MLST or PFGE. Copyright © 2017 Elsevier B.V. All rights reserved.

  6. Methods of assessing functioning of organizational and economic mechanism during innovation process implementation

    Directory of Open Access Journals (Sweden)

    Blinkov Maksim

    2017-01-01

    Full Text Available This paper proposes methods of assessing the efficiency of organizational and economic mechanism of an industrial enterprise when implementing innovation processes. These methods allow continuous monitoring at all stages of innovation process implementation, lead to reduction in costs of innovation activity and enable maximum use of the creative potential of enterprise personnel. The significance and attractiveness of this method is ensured by the fact that it can be applied by industrial companies in any market fields regardless of the lifecycle stage applicable to the studied goods, company and/or innovative process because the composition and the number of specific indicators can be adjusted by the work group both before the study and in the course of the company’s innovative activities (at any stage of their implementation. The multi-sided approach proposed for assessing the efficiency of organizational and economic mechanism of the industrial enterprise when implementing innovation processes ensures full and accurate assessment of the impact of certain factors on the final result.

  7. Quantum cloning machines and their implementation in physical systems

    International Nuclear Information System (INIS)

    Wu Tao; Ye Liu; Fang Bao-Long

    2013-01-01

    We review the basic theory of approximate quantum cloning for discrete variables and some schemes for implementing quantum cloning machines. Several types of approximate quantum clones and their expansive quantum clones are introduced. As for the implementation of quantum cloning machines, we review some design methods and recent experimental results. (topical review - quantum information)

  8. Work system innovation: Designing improvement methods for generative capability

    DEFF Research Database (Denmark)

    Hansen, David; Møller, Niels

    2013-01-01

    This paper explores how a work system’s capability for improvement is influenced by its improvement methods. Based on explorative case study at a Lean manufacturing facility, the methods problem solving and Appreciative Inquiry were compared through in-depth qualitative studies over a 12-month...

  9. RESEARCH ARTICLE Molecular cloning and functional ...

    Indian Academy of Sciences (India)

    Navya

    2016-11-25

    Nov 25, 2016 ... Molecular cloning and functional characterization of two novel ... Currently, many variants of HMW-GSs have been cloned from bread wheat .... SDS sedimentation tests were conducted using the methods described by Gao et ...

  10. Feasibility study for an innovative industrial red mud utilisation method.

    Science.gov (United States)

    Kounalakis, Petros; Aravossis, Konstantinos; Karayianni, ChS

    2016-02-01

    Red mud is a high volume industrial waste, and its management poses a unique challenge. For the utilisation of red mud, an economical, energy saving, environmental friendly and widely applicable method has been found. The proposed novel method is purely chemical, and achieves the recovery of all the oxides contained in red mud totally and transforms them into high value added products. The present work shows that an investment in an industrial plant, treating red mud and turning a toxic industrial waste in commercial products, is safe and viable. © The Author(s) 2015.

  11. The Innovation Blaze-Method of Development Professional Thinking Designers in the Modern Higher Education

    Science.gov (United States)

    Alekseeva, Irina V.; Barsukova, Natalia I.; Pallotta, Valentina I.; Skovorodnikova, Nadia A.

    2017-01-01

    This article proved the urgency of the problem of development of professional thinking of students studying design in modern conditions of higher education. The authors substantiate for the need of an innovative Blaise-method development of professional design thinking of students in higher education. "Blaise-method" named by us in…

  12. Human Cloning

    Science.gov (United States)

    2006-07-20

    Human Fertilization and Embryology Authority (HFEA). A team of scientists headed by Alison Murdoch at the University of Newcastle received permission...not yet reported success in isolating stem cells from a cloned human embryo. A research team headed by Ian Wilmut at the University of Edinburgh...research group, headed by Douglas Melton and Kevin Eggan, submitted their proposal to a Harvard committee composed of ethicists, scientists and public

  13. Innovative Teaching Practice: Traditional and Alternative Methods (Challenges and Implications)

    Science.gov (United States)

    Nurutdinova, Aida R.; Perchatkina, Veronika G.; Zinatullina, Liliya M.; Zubkova, Guzel I.; Galeeva, Farida T.

    2016-01-01

    The relevance of the present issue is caused be the strong need in alternative methods of learning foreign language and the need in language training and retraining for the modern professionals. The aim of the article is to identify the basic techniques and skills in using various modern techniques in the context of modern educational tasks. The…

  14. Methods for innovation : the varying role of industrial research in DSM's nitrogen fertilizer business, 1925-1970

    NARCIS (Netherlands)

    Rooij, van J.W.

    2007-01-01

    In this article the varying methods firms use to innovate are central in an attempt to balance our understanding of innovation processes. Often, research-driven innovation attracts the most attention, obscuring the contributions of other company functions and of external technology. In this article,

  15. CRISPR-Cas9-Edited Site Sequencing (CRES-Seq): An Efficient and High-Throughput Method for the Selection of CRISPR-Cas9-Edited Clones.

    Science.gov (United States)

    Veeranagouda, Yaligara; Debono-Lagneaux, Delphine; Fournet, Hamida; Thill, Gilbert; Didier, Michel

    2018-01-16

    The emergence of clustered regularly interspaced short palindromic repeats-Cas9 (CRISPR-Cas9) gene editing systems has enabled the creation of specific mutants at low cost, in a short time and with high efficiency, in eukaryotic cells. Since a CRISPR-Cas9 system typically creates an array of mutations in targeted sites, a successful gene editing project requires careful selection of edited clones. This process can be very challenging, especially when working with multiallelic genes and/or polyploid cells (such as cancer and plants cells). Here we described a next-generation sequencing method called CRISPR-Cas9 Edited Site Sequencing (CRES-Seq) for the efficient and high-throughput screening of CRISPR-Cas9-edited clones. CRES-Seq facilitates the precise genotyping up to 96 CRISPR-Cas9-edited sites (CRES) in a single MiniSeq (Illumina) run with an approximate sequencing cost of $6/clone. CRES-Seq is particularly useful when multiple genes are simultaneously targeted by CRISPR-Cas9, and also for screening of clones generated from multiallelic genes/polyploid cells. © 2018 by John Wiley & Sons, Inc. Copyright © 2018 John Wiley & Sons, Inc.

  16. Inverse fusion PCR cloning.

    Directory of Open Access Journals (Sweden)

    Markus Spiliotis

    Full Text Available Inverse fusion PCR cloning (IFPC is an easy, PCR based three-step cloning method that allows the seamless and directional insertion of PCR products into virtually all plasmids, this with a free choice of the insertion site. The PCR-derived inserts contain a vector-complementary 5'-end that allows a fusion with the vector by an overlap extension PCR, and the resulting amplified insert-vector fusions are then circularized by ligation prior transformation. A minimal amount of starting material is needed and experimental steps are reduced. Untreated circular plasmid, or alternatively bacteria containing the plasmid, can be used as templates for the insertion, and clean-up of the insert fragment is not urgently required. The whole cloning procedure can be performed within a minimal hands-on time and results in the generation of hundreds to ten-thousands of positive colonies, with a minimal background.

  17. PHIRE (Public Health Innovation and Research in Europe): methods, structures and evaluation.

    Science.gov (United States)

    Barnhoorn, Floris; McCarthy, Mark; Devillé, Walter; Alexanderson, Kristina; Voss, Margaretha; Conceição, Claudia

    2013-11-01

    Public Health Innovation and Research in Europe (PHIRE), building on previous European collaborative projects, was developed to assess national uptake and impacts of European public health innovations, to describe national public health research programmes, strategies and structures and to develop participation of researchers through the organizational structures of the European Public Health Association (EUPHA). This article describes the methods used. PHIRE was led by EUPHA with seven partner organisations over 30 months. It was conceived to engage the organisation of EUPHA--working through its thematic Sections, and through its national public health associations--and assess innovation and research across 30 European countries. Public health research was defined broadly as health research at population and organisational level. There were seven Work Packages (three covering coordination and four for technical aspects) led by partners and coordinated through management meetings. Seven EUPHA Sections identified eight innovations within the projects funded by the Public Health Programme of the European Commission Directorate for Health and Consumers. Country informants, identified through EUPHA thematic Sections, reported on national uptake of the innovations in eight public health projects supported by the European Union Public Health Programme. Four PHIRE partners, each taking a regional sector of Europe, worked with the public health associations and other informants to describe public health research programmes, calls and systems. A classification was created for the national public health research programmes and calls in 2010. The internal and external evaluations were supportive. PHIRE described public health innovations and research across Europe through national experts. More work is needed to conceptualize and define public health 'innovations' and to develop theories and methods for the assessment of their uptake and impacts at country and cross

  18. Recent and innovative methods for detection of bacteremia and fungemia

    International Nuclear Information System (INIS)

    Reller, L.B.

    1983-01-01

    Advances continue to be made in methods for more reliable or more rapid means of detecting bacteremia and fungemia. The importance of blood sample volume and broth dilution has been established in controlled studies. New technology includes the use of resins that remove antimicrobials from blood samples, detection of radioactivity from organisms given radiolabeled substrate, use of dyes that stain microbial DNA and RNA, use of slides coated with growth media, and lysis-centrifugation for trapping microorganisms. Technology now being considered includes counterimmunoelectrophoresis, head-space gas chromatography, electrical impedance, microcalorimetry, and the use of lasers to detect pH changes and turbidity

  19. Innovative Methods in Strategic Planning of Foreign Economic Activity of Enterprises

    Directory of Open Access Journals (Sweden)

    Valentyna Yakubiv

    2017-12-01

    Full Text Available Changes in the external conditions of business in Ukraine, European integration vector of the country development, and possibilities of import/export operations facilitate foreign trade. Modern methods of strategic management and strategic diagnostics should be developed in line with international requirements and needs of managers in Ukraine and partners abroad. Therefore substantiation of innovation trends for enterprise development strategies on the international arena as a constituent of stable economy is important and necessary. The purpose of this article is to define innovative approaches to planning, development and implementation of strategies and to develop a comprehensive diagnosis of the strategic potential of foreign economic activity. During the research, the following methods: logical abstraction, modeling, PEST-analysis, systems analysis, graphic. The main results of the study are: 1 disclosure of the features of the construction of strategic management in international business; 2 analysis of European and American practices of strategic management of foreign economic activity of enterprises; 3 study of algorithmic scheme of strategic management in the Ukraine; 4 justification of methods of three-level system of strategic analysis using matrix methods of assessment; 5 the classification of the main methods of matrix analysis within groups: macro, meso, macro level; 6 a comprehensive diagnosis of the strategic potential of foreign economic activity of enterprises in Ukraine. The study proved the main areas of innovation and strategic principles of international management and comprehensive methodology for strategic planning matrix based on innovative methods.

  20. Method for innovative synthesis-design of chemical process flowsheets

    DEFF Research Database (Denmark)

    Kumar Tula, Anjan; Gani, Rafiqul

    Chemical process synthesis-design involve the identification of the processing route to reach a desired product from a specified set of raw materials, design of the operations involved in the processing route, the calculations of utility requirements, the calculations of waste and emission...... to the surrounding and many more. Different methods (knowledge-based [1], mathematical programming [2], hybrid, etc.) have been proposed and are also currently employed to solve these synthesis-design problems. D’ Anterroches [3] proposed a group contribution based approach to solve the synthesis-design problem...... of chemical processes, where, chemical process flowsheets could be synthesized in the same way as atoms or groups of atoms are synthesized to form molecules in computer aided molecular design (CAMD) techniques [4]. That, from a library of building blocks (functional process-groups) and a set of rules to join...

  1. Educational research methods for researching innovations in teaching, learning and assessment: The nursing lecturer as researcher.

    Science.gov (United States)

    Marks-Maran, Diane

    2015-11-01

    The author, who has had previous experience as a nurse researcher, has been engaged in helping nurse lecturers to undertake evaluation research studies into innovations in their teaching, learning and assessment methods. In order to undertake this work successfully, it was important to move from thinking like a nurse researcher to thinking like an educational researcher and developing the role of the nursing lecturer as researcher of their teaching. This article explores the difference between evaluation and evaluation research and argues for the need to use educational research methods when undertaking evaluation research into innovations in teaching, learning and assessment. A new model for educational evaluation research is presented together with two case examples of the model in use. The model has been tested on over 30 research studies into innovations in teaching, learning and assessment over the past 8 years. Copyright © 2015 Elsevier Ltd. All rights reserved.

  2. Innovative study methods for the Mediterranean coralligenous habitats

    Directory of Open Access Journals (Sweden)

    P.A. Zapata-Ramírez

    2013-11-01

    Full Text Available Coralligenous habitats are of special interest in the Mediterranean Sea because they represent one of the most important biodiversity ‘hot-spots’ and are considered of great relevance for fisheries activities in the region. Despite their importance, however, there are missing consensual methodologies for their monitoring and, despite some attempts, no environmental or ecological quality indices have been established yet. This situation could be related to the difficulties associated with their exploration and their spatial heterogeneity. These habitats are in urgent need of efficient standard monitoring and management protocols programmes to develop an effective network for their conservation. Here we reviewed the available methodologies and robotics tools used to evaluate and monitor benthic habitats, highlighting the importance of defining rapid cost-effective sampling and analyses approaches and architectures for future monitoring of changes in coralligenous habitats based on current technological developments. We identified still images acquisitions as the most effective data gathering system. Stereo photogrammetry, photomosaic elaboration and three-dimensional (3D modelling may largely improve the data analysis and therefore the quality status assessment of the coralligenous habitats. The advantage and efficiency of different approaches and methods, and whether they should be applied and standardised for further monitoring activities, were discussed.

  3. Innovative methods of knowledge transfer by multimedia library

    Science.gov (United States)

    Goanta, A. M.

    2016-08-01

    The present situation of teaching and learning new knowledge taught in the classroom is highly variable depending on the specific topics concerned. If we analyze the manifold ways of teaching / learning at university level, we can notice a very good combination between classical and modern methods. The first category includes the classic chalk blackboard teaching, followed by the also classical learning based on paper reference material. The second category includes books published in PDF or PPT [1], which are printed on the type backing CD / DVD. Since 2006 the author was concerned about the transfer of information and knowledge through video files like AVI, FLV or MPEG using various means of transfer, from the free ones (via Internet) and continuing with those involving minimal costs, i.e. on CD / DVD support. Encouraged by the students’ interest in this kind of teaching material as proved by monitoring [2] the site http://www.cursuriuniversitarebraila.ugal.ro, the author has managed to publish with ISBN the first video book in Romania, which has a non conformist content in that the chapters are located not by paging but by the hour or minutes of shooting when they were made.

  4. Innovative research methods for studying treatments for rare diseases: methodological review.

    Science.gov (United States)

    Gagne, Joshua J; Thompson, Lauren; O'Keefe, Kelly; Kesselheim, Aaron S

    2014-11-24

    To examine methods for generating evidence on health outcomes in patients with rare diseases. Methodological review of existing literature. PubMed, Embase, and Academic Search Premier searched for articles describing innovative approaches to randomized trial design and analysis methods and methods for conducting observational research in patients with rare diseases. We assessed information related to the proposed methods, the specific rare disease being studied, and outcomes from the application of the methods. We summarize methods with respect to their advantages in studying health outcomes in rare diseases and provide examples of their application. We identified 46 articles that proposed or described methods for studying patient health outcomes in rare diseases. Articles covered a wide range of rare diseases and most (72%) were published in 2008 or later. We identified 16 research strategies for studying rare disease. Innovative clinical trial methods minimize sample size requirements (n=4) and maximize the proportion of patients who receive active treatment (n=2), strategies crucial to studying small populations of patients with limited treatment choices. No studies describing unique methods for conducting observational studies in patients with rare diseases were identified. Though numerous studies apply unique clinical trial designs and considerations to assess patient health outcomes in rare diseases, less attention has been paid to innovative methods for studying rare diseases using observational data. © Gagne et al 2014.

  5. Effectiveness of Various Innovative Learning Methods in Health Science Classrooms: A Meta-Analysis

    Science.gov (United States)

    Kalaian, Sema A.; Kasim, Rafa M.

    2017-01-01

    This study reports the results of a meta-analysis of the available literature on the effectiveness of various forms of innovative small-group learning methods on student achievement in undergraduate college health science classrooms. The results of the analysis revealed that most of the primary studies supported the effectiveness of the…

  6. Developing Critical Understanding in HRM Students: Using Innovative Teaching Methods to Encourage Deep Approaches to Study

    Science.gov (United States)

    Butler, Michael J. R.; Reddy, Peter

    2010-01-01

    Purpose: This paper aims to focus on developing critical understanding in human resource management (HRM) students in Aston Business School, UK. The paper reveals that innovative teaching methods encourage deep approaches to study, an indicator of students reaching their own understanding of material and ideas. This improves student employability…

  7. Co-Designing and Co-Teaching Graduate Qualitative Methods: An Innovative Ethnographic Workshop Model

    Science.gov (United States)

    Cordner, Alissa; Klein, Peter T.; Baiocchi, Gianpaolo

    2012-01-01

    This article describes an innovative collaboration between graduate students and a faculty member to co-design and co-teach a graduate-level workshop-style qualitative methods course. The goal of co-designing and co-teaching the course was to involve advanced graduate students in all aspects of designing a syllabus and leading class discussions in…

  8. Key Competencies and Characteristics for Innovative Teaching among Secondary School Teachers: A Mixed-Methods Research

    Science.gov (United States)

    Zhu, Chang; Wang, Di

    2014-01-01

    This research aims to understand the key competencies and characteristics for innovative teaching as perceived by Chinese secondary teachers. A mixed-methods research was used to investigate secondary teachers' views. First, a qualitative study was conducted with interviews of teachers to understand the perceived key competencies and…

  9. From individual innovation to global impact: the Global Cooperation on Assistive Technology (GATE) innovation snapshot as a method for sharing and scaling.

    Science.gov (United States)

    Layton, Natasha; Murphy, Caitlin; Bell, Diane

    2018-05-09

    Assistive technology (AT) is an essential facilitator of independence and participation, both for people living with the effects of disability and/or non-communicable disease, as well as people aging with resultant functional decline. The World Health Organization (WHO) recognizes the substantial gap between the need for and provision of AT and is leading change through the Global Cooperation on Assistive Technology (GATE) initiative. Showcasing innovations gathered from 92 global researchers, innovators, users and educators of AT through the WHO GREAT Summit, this article provides an analysis of ideas and actions on a range of dimensions in order to provide a global overview of AT innovation. The accessible method used to capture and showcase this data is presented and critiqued, concluding that "innovation snapshots" are a rapid and concise strategy to capture and showcase AT innovation and to foster global collaboration. Implications for Rehabilitation Focal tools such as ePosters with uniform data requirements enable the rapid sharing of information. A diversity of innovative practices are occurring globally in the areas of AT Products, Policy, Provision, People and Personnel. The method offered for Innovation Snapshots had substantial uptake and is a feasible means to capture data across a range of stakeholders. Meeting accessibility criteria is an emerging competency in the AT community. Substantial areas of common interest exist across regions and globally in the AT community, demonstrating the effectiveness of information sharing platforms such as GATE and supporting the idea of regional forums and networks.

  10. [Scientific ethics of human cloning].

    Science.gov (United States)

    Valenzuela, Carlos Y

    2005-01-01

    True cloning is fission, budding or other types of asexual reproduction. In humans it occurs in monozygote twinning. This type of cloning is ethically and religiously good. Human cloning can be performed by twinning (TWClo) or nuclear transfer (NTClo). Both methods need a zygote or a nuclear transferred cell, obtained in vitro (IVTec). They are under the IVTec ethics. IVTecs use humans (zygotes, embryos) as drugs or things; increase the risk of malformations; increase development and size of abnormalities and may cause long-term changes. Cloning for preserving extinct (or almost extinct) animals or humans when sexual reproduction is not possible is ethically valid. The previous selection of a phenotype in human cloning violates some ethical principles. NTClo for reproductive or therapeutic purposes is dangerous since it increases the risk for nucleotide or chromosome mutations, de-programming or re-programming errors, aging or malignancy of the embryo cells thus obtained.

  11. DEVELOPMENT OF ASSESSMENT METHODS OF EFFECTIVENESS OF INNOVATIVE STAFF ACTIVITY MOTIVATIONAL MECHANISM

    Directory of Open Access Journals (Sweden)

    Viktoriia Honchar

    2016-11-01

    Full Text Available The aim of the science work is to develop methods to assess the innovative activity of the personnel that will provide motivation for employees to be active in the direction of research and introduction of internal reserves for its improvement. Methods. In the study were used: system method and methods of logical analysis – to justify theoretical and practical recommendations on the development of conceptual bases of the formation of the motivational mechanism of personnel management, economic-mathematical modelling and forecasting methods – to assess the influence of motivation factors on the level of labour activity of the personnel. Results. In the work the notion “motivation”, considering the innovative changes of the modern economy, is clarified. In order to foster innovative activity in the enterprise it is proposed to improve organizational structure by controlling the center of innovation activity management, which includes economic, technical and social division. Proposed an establishment of the project teams under the terms of accounting costs, which contributes to more active workers’ involvement in the formation of innovative development plans. The main points that determine their effectiveness are: growth of volumes of output, diminution of expenses of materials and energy resources, timeliness and relevance to the product market, improvement of the quality of work performed. A profit, derived by project teams, is recommended to distribute on: increment of the payroll, innovative development, stimulation and motivation fund. The research of the activity of the enterprises showed that one of the effective methods to stimulate staff is a motivational system based on the use of KPI. Bonuses for the implemented project, which are adjusted to the output of the basic stage, or KPI project as a whole, fixed bonuses as a percentage of the profits, and bonuses in stages of the project are the forms of motivation of project teams

  12. Gene design, cloning and protein-expression methods for high-value targets at the Seattle Structural Genomics Center for Infectious Disease

    International Nuclear Information System (INIS)

    Raymond, Amy; Haffner, Taryn; Ng, Nathan; Lorimer, Don; Staker, Bart; Stewart, Lance

    2011-01-01

    An overview of one salvage strategy for high-value SSGCID targets is given. Any structural genomics endeavor, particularly ambitious ones such as the NIAID-funded Seattle Structural Genomics Center for Infectious Disease (SSGCID) and Center for Structural Genomics of Infectious Disease (CSGID), face technical challenges at all points of the production pipeline. One salvage strategy employed by SSGCID is combined gene engineering and structure-guided construct design to overcome challenges at the levels of protein expression and protein crystallization. Multiple constructs of each target are cloned in parallel using Polymerase Incomplete Primer Extension cloning and small-scale expressions of these are rapidly analyzed by capillary electrophoresis. Using the methods reported here, which have proven particularly useful for high-value targets, otherwise intractable targets can be resolved

  13. The Innovative Bike Conceptual Design by Using Modified Functional Element Design Method

    Directory of Open Access Journals (Sweden)

    Nien-Te Liu

    2016-11-01

    Full Text Available The purpose of the study is to propose a new design process by modifying functional element design approach which can commence a large amount of innovative concepts within a short period of time. Firstly, the original creative functional elements design method is analyzed and the drawbacks are discussed. Then, the modified is proposed and is divided into 6 steps. The creative functional element representations, generalization, specialization, and particularization are used in this method. Every step is described clearly, and users could design by following the process easily. In this paper, a clear and accurate design process is proposed based on the creative functional element design method. By following this method, a lot of innovative bicycles will be created quickly.

  14. Modeling technology innovation: how science, engineering, and industry methods can combine to generate beneficial socioeconomic impacts.

    Science.gov (United States)

    Stone, Vathsala I; Lane, Joseph P

    2012-05-16

    Government-sponsored science, technology, and innovation (STI) programs support the socioeconomic aspects of public policies, in addition to expanding the knowledge base. For example, beneficial healthcare services and devices are expected to result from investments in research and development (R&D) programs, which assume a causal link to commercial innovation. Such programs are increasingly held accountable for evidence of impact-that is, innovative goods and services resulting from R&D activity. However, the absence of comprehensive models and metrics skews evidence gathering toward bibliometrics about research outputs (published discoveries), with less focus on transfer metrics about development outputs (patented prototypes) and almost none on econometrics related to production outputs (commercial innovations). This disparity is particularly problematic for the expressed intent of such programs, as most measurable socioeconomic benefits result from the last category of outputs. This paper proposes a conceptual framework integrating all three knowledge-generating methods into a logic model, useful for planning, obtaining, and measuring the intended beneficial impacts through the implementation of knowledge in practice. Additionally, the integration of the Context-Input-Process-Product (CIPP) model of evaluation proactively builds relevance into STI policies and programs while sustaining rigor. The resulting logic model framework explicitly traces the progress of knowledge from inputs, following it through the three knowledge-generating processes and their respective knowledge outputs (discovery, invention, innovation), as it generates the intended socio-beneficial impacts. It is a hybrid model for generating technology-based innovations, where best practices in new product development merge with a widely accepted knowledge-translation approach. Given the emphasis on evidence-based practice in the medical and health fields and "bench to bedside" expectations for

  15. DNA cloning: a practical approach. Volume 1

    Energy Technology Data Exchange (ETDEWEB)

    Glover, D M [ed.

    1985-01-01

    This book is written for the advanced molecular biologist who needs a detailed discussion of cloning technology. Topics of discussion include: genomic library cloning (size of a genomic library, screening methods, chromosome walking, host cell genetics, and general features of bacteriophage Iambda); use of gt10 and gt11 cDNA lambda vectors and general cDNA cloning; RNase H-Pol I cDNA synthesis; method of detecting fusion proteins produced in bacteria; pEMBL family of double-stranded plasmid vectors that can be used to generate single strands; Escherichia coli transformation; production of mutations in cloned sequences; and cloning in gram negative bacteria.

  16. Human Cloning: Let's Discuss It.

    Science.gov (United States)

    Taras, Loretta; Stavroulakis, Anthea M.; Ortiz, Mary T.

    1999-01-01

    Describes experiences with holding discussions on cloning at a variety of levels in undergraduate biology courses. Discusses teaching methods used and student reactions to the discussions. Contains 12 references. (WRM)

  17. An innovative jet boring mining method available for the high grade uranium ore underground deposits

    International Nuclear Information System (INIS)

    Narcy, J.L.

    1996-01-01

    An innovative mining method, based on the capability of a high pressure water jet to desaggregate rock, has been conceived and tested with success at the highest grade uranium ore deposit in the world, the Cigar Lake deposit in Saskatchewan, Canada. 113 tonnes of ore at 13% U were mined out by a new jet-boring mining method operated on a semi-industrial basis, in 1992 during the test mining program of Cigar Lake Project. (author). 9 figs

  18. The myths of innovation

    National Research Council Canada - National Science Library

    Berkun, Scott

    2007-01-01

    ... . . . . . . . . . . . . . . . . . . . . . . 1 Chapter 2 We understand the history of innovation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17 Chapter 3 There is a method for innovation...

  19. Current status and trends, organizational methods accounting and analysis of innovative enterprises’ venture financing

    Directory of Open Access Journals (Sweden)

    S.V. Rudeychuk

    2016-09-01

    Full Text Available The article examines the specific features and tendencies of development of venture financing in Ukraine. It shows the role of venture capital in the further development of Ukraine economy, this role is in financing and development of innovation, distribution of new technologies and increase of employment of highly professional staff in the territory of Ukraine. The authors determine the influence of characteristics of such financing on the formation of organization and methods of accounting and economic analysis of operations with venture capital investments. The paper grounds the necessity of accounting development and analytical support of innovative enterprises’ venture financing management; the state is caused by the need to attract additional funding from venture investors to finance the production and release of innovative products and businesses. The authors determine the directions of solving problems in accounting organization and methods and economic analysis of innovative enterprises’ venture financing, respectively, for the needs of all entities doing business venture and taking into account the peculiarities of this type of investment capital.

  20. RESULTS OF ANALYSIS OF BENCHMARKING METHODS OF INNOVATION SYSTEMS ASSESSMENT IN ACCORDANCE WITH AIMS OF SUSTAINABLE DEVELOPMENT OF SOCIETY

    Directory of Open Access Journals (Sweden)

    A. Vylegzhanina

    2016-01-01

    Full Text Available In this work, we introduce results of comparative analysis of international ratings indexes of innovation systems for their compliance with purposes of sustainable development. Purpose of this research is defining requirements to benchmarking methods of assessing national or regional innovation systems and compare them basing on assumption, that innovation system is aligned with sustainable development concept. Analysis of goal sets and concepts, which underlie observed international composite innovation indexes, comparison of their metrics and calculation techniques, allowed us to reveal opportunities and limitations of using these methods in frames of sustainable development concept. We formulated targets of innovation development on the base of innovation priorities of sustainable socio-economic development. Using comparative analysis of indexes with these targets, we revealed two methods of assessing innovation systems, maximally connected with goals of sustainable development. Nevertheless, today no any benchmarking method, which meets need of innovation systems assessing in compliance with sustainable development concept to a sufficient extent. We suggested practical directions of developing methods, assessing innovation systems in compliance with goals of societal sustainable development.

  1. Hybrid sequencing approach applied to human fecal metagenomic clone libraries revealed clones with potential biotechnological applications.

    Science.gov (United States)

    Džunková, Mária; D'Auria, Giuseppe; Pérez-Villarroya, David; Moya, Andrés

    2012-01-01

    Natural environments represent an incredible source of microbial genetic diversity. Discovery of novel biomolecules involves biotechnological methods that often require the design and implementation of biochemical assays to screen clone libraries. However, when an assay is applied to thousands of clones, one may eventually end up with very few positive clones which, in most of the cases, have to be "domesticated" for downstream characterization and application, and this makes screening both laborious and expensive. The negative clones, which are not considered by the selected assay, may also have biotechnological potential; however, unfortunately they would remain unexplored. Knowledge of the clone sequences provides important clues about potential biotechnological application of the clones in the library; however, the sequencing of clones one-by-one would be very time-consuming and expensive. In this study, we characterized the first metagenomic clone library from the feces of a healthy human volunteer, using a method based on 454 pyrosequencing coupled with a clone-by-clone Sanger end-sequencing. Instead of whole individual clone sequencing, we sequenced 358 clones in a pool. The medium-large insert (7-15 kb) cloning strategy allowed us to assemble these clones correctly, and to assign the clone ends to maintain the link between the position of a living clone in the library and the annotated contig from the 454 assembly. Finally, we found several open reading frames (ORFs) with previously described potential medical application. The proposed approach allows planning ad-hoc biochemical assays for the clones of interest, and the appropriate sub-cloning strategy for gene expression in suitable vectors/hosts.

  2. Hybrid sequencing approach applied to human fecal metagenomic clone libraries revealed clones with potential biotechnological applications.

    Directory of Open Access Journals (Sweden)

    Mária Džunková

    Full Text Available Natural environments represent an incredible source of microbial genetic diversity. Discovery of novel biomolecules involves biotechnological methods that often require the design and implementation of biochemical assays to screen clone libraries. However, when an assay is applied to thousands of clones, one may eventually end up with very few positive clones which, in most of the cases, have to be "domesticated" for downstream characterization and application, and this makes screening both laborious and expensive. The negative clones, which are not considered by the selected assay, may also have biotechnological potential; however, unfortunately they would remain unexplored. Knowledge of the clone sequences provides important clues about potential biotechnological application of the clones in the library; however, the sequencing of clones one-by-one would be very time-consuming and expensive. In this study, we characterized the first metagenomic clone library from the feces of a healthy human volunteer, using a method based on 454 pyrosequencing coupled with a clone-by-clone Sanger end-sequencing. Instead of whole individual clone sequencing, we sequenced 358 clones in a pool. The medium-large insert (7-15 kb cloning strategy allowed us to assemble these clones correctly, and to assign the clone ends to maintain the link between the position of a living clone in the library and the annotated contig from the 454 assembly. Finally, we found several open reading frames (ORFs with previously described potential medical application. The proposed approach allows planning ad-hoc biochemical assays for the clones of interest, and the appropriate sub-cloning strategy for gene expression in suitable vectors/hosts.

  3. The Water-Energy-Food Nexus: Advancing Innovative, Policy-Relevant Methods

    Science.gov (United States)

    Crootof, A.; Albrecht, T.; Scott, C. A.

    2017-12-01

    The water-energy-food (WEF) nexus is rapidly expanding in scholarly literature and policy settings as a novel way to address complex Anthropocene challenges. The nexus approach aims to identify tradeoffs and synergies of water, energy, and food systems, internalize social and environmental impacts, and guide development of cross-sectoral policies. However, a primary limitation of the nexus approach is the absence - or gaps and inconsistent use - of adequate methods to advance an innovative and policy-relevant nexus approach. This paper presents an analytical framework to identify robust nexus methods that align with nexus thinking and highlights innovative nexus methods at the frontier. The current state of nexus methods was assessed with a systematic review of 245 journal articles and book chapters. This review revealed (a) use of specific and reproducible methods for nexus assessment is uncommon - less than one-third of the reviewed studies present explicit methods; (b) nexus methods frequently fall short of capturing interactions among water, energy, and food - the very concept they purport to address; (c) assessments strongly favor quantitative approaches - 70% use primarily quantitative tools; (d) use of social science methods is limited (26%); and (e) many nexus methods are confined to disciplinary silos - only about one-quarter combine methods from diverse disciplines and less than one-fifth utilize both quantitative and qualitative approaches. Despite some pitfalls of current nexus methods, there are a host of studies that offer innovative approaches to help quantify nexus linkages and interactions among sectors, conceptualize dynamic feedbacks, and support mixed method approaches to better understand WEF systems. Applying our analytical framework to all 245 studies, we identify, and analyze herein, seventeen studies that implement innovative multi-method and cross-scalar tools to demonstrate promising advances toward improved nexus assessment. This paper

  4. An Innovative Teaching Method To Promote Active Learning: Team-Based Learning

    Science.gov (United States)

    Balasubramanian, R.

    2007-12-01

    Traditional teaching practice based on the textbook-whiteboard- lecture-homework-test paradigm is not very effective in helping students with diverse academic backgrounds achieve higher-order critical thinking skills such as analysis, synthesis, and evaluation. Consequently, there is a critical need for developing a new pedagogical approach to create a collaborative and interactive learning environment in which students with complementary academic backgrounds and learning skills can work together to enhance their learning outcomes. In this presentation, I will discuss an innovative teaching method ('Team-Based Learning (TBL)") which I recently developed at National University of Singapore to promote active learning among students in the environmental engineering program with learning abilities. I implemented this new educational activity in a graduate course. Student feedback indicates that this pedagogical approach is appealing to most students, and promotes active & interactive learning in class. Data will be presented to show that the innovative teaching method has contributed to improved student learning and achievement.

  5. Characterization of an innovative method for RuO2 deposition using Electron Microscopy

    DEFF Research Database (Denmark)

    Cavalca, Filippo

    Many photocatalysts work better or exclusively when a suitable cocatalyst, such as RuO2, is deposited on their surface. An innovative method of RuO2 deposition has been found to improve the performance of photocatalysts such as (Ga1-xZnx)(N1-xOx), WO3, SrTiO3 and TiO2. Here we use high angle annu...

  6. An Innovative Oil Pollution Containment Method for Ship Wrecks Proposed for Offshore Well Blow-outs

    OpenAIRE

    ANDRITSOS Fivos; COJINS Hans

    2011-01-01

    In the aftermath of the PRESTIGE disaster, an innovative system for the prompt intervention on oil pollution sources (primarily ship wrecks) at great depths was conceived at the Joint Research Center of the European Commission. This system, with some re-engineering, could also serve for collecting oil and gas leaking after an offshore well blow-out and could constitute a reference method for prompt intervention on deep water oil pollution sources like ship wrecks and blown-out offshore wells....

  7. METHODICAL APPROACHES TO FORMING INNOVATIVE PROJECT AND ENTERPRISE MANAGEMENT ORGANIZATIONAL STRUCTURES

    Directory of Open Access Journals (Sweden)

    A. O. Baklanov

    2011-01-01

    Full Text Available The following principles are discussed as methodical bases of the choice of innovative project and enterprise management organizational structures: hierarchism, bureaucratization, adhocratie, tent organization, lean business engineering, rational organization, amorphous organization, teaching organization. Conditions that complicate industrial and technological processes influence enterprise management process dynamics. The variety of existing types of organizational structures is determined by industrial, economic, psychological and social conditions.

  8. INNOVATIVE METHODS TO EVALUATE THE RELIABILITY OF INFORMATION CONSOLIDATED FINANCIAL STATEMENTS

    Directory of Open Access Journals (Sweden)

    Irina P. Kurochkina

    2014-01-01

    Full Text Available The article explores the possibility of using foreign innovative methods to assess the reliabilityof information consolidated fi nancial statements of Russian companies. Recommendations aremade under their adaptation and applicationinto commercial organizations. Banish methodindicators are implemented in one of the world’s largest vertically integrated steel and miningcompanies. Audit firms are proposed to usemethods of assessing the reliability of information in the practical application of ISA.

  9. INNOVATIVE FORMS SUPPORTING SAFE METHODS OF WORK IN SAFETY ENGINEERING FOR THE DEVELOPMENT OF INTELLIGENT SPECIALIZATIONS

    Directory of Open Access Journals (Sweden)

    Anna GEMBALSKA-KWIECIEŃ

    2016-10-01

    Full Text Available The article discusses innovative forms of participation of employees in the work safety system. It also presents the advantages of these forms of employees’ involvement. The aim of empirical studies was the analysis of their behavior and attitude towards health and safety at work. The issues considered in the article have a significant impact on the improvement of methods of prevention related to work safety and aided the creation of a healthy society.

  10. Social networking and young adults’ drinking practices:innovative qualitative methods for health behavior research

    OpenAIRE

    Lyons, Antonia C.; Goodwin, Ian; McCreanor, Tim; Griffin, Christine

    2015-01-01

    Understandings of health behaviours can be enriched by employing innovative qualitative research designs. We draw on research that employed a range of qualitative methods to explore young adults’ drinking behaviours and social networking in Aotearoa New Zealand. We demonstrate the importance of multiple research approaches to situate drinking practices within their broader social context. The research involved three data collection stages. First, 34 friendship focus group discussions were con...

  11. Effectiveness of various innovative learning methods in health science classrooms: a meta-analysis.

    Science.gov (United States)

    Kalaian, Sema A; Kasim, Rafa M

    2017-12-01

    This study reports the results of a meta-analysis of the available literature on the effectiveness of various forms of innovative small-group learning methods on student achievement in undergraduate college health science classrooms. The results of the analysis revealed that most of the primary studies supported the effectiveness of the small-group learning methods in improving students' academic achievement with an overall weighted average effect-size of 0.59 in standard deviation units favoring small-group learning methods. The subgroup analysis showed that the various forms of innovative and reform-based small-group learning interventions appeared to be significantly more effective for students in higher levels of college classes (sophomore, junior, and senior levels), students in other countries (non-U.S.) worldwide, students in groups of four or less, and students who choose their own group. The random-effects meta-regression results revealed that the effect sizes were influenced significantly by the instructional duration of the primary studies. This means that studies with longer hours of instruction yielded higher effect sizes and on average every 1 h increase in instruction, the predicted increase in effect size was 0.009 standard deviation units, which is considered as a small effect. These results may help health science and nursing educators by providing guidance in identifying the conditions under which various forms of innovative small-group learning pedagogies are collectively more effective than the traditional lecture-based teaching instruction.

  12. Clonal analysis of the T-cell response to in vivo expressed Mycobacterium tuberculosis protein Rv2034, using a CD154 expression based T-cell cloning method.

    Directory of Open Access Journals (Sweden)

    Susanna Commandeur

    Full Text Available Tuberculosis (TB, caused by Mycobacterium tuberculosis (Mtb, remains a leading cause of death worldwide. A better understanding of the role of CD4+ and CD8+ T cells, which are both important to TB protection, is essential to unravel the mechanisms of protection and to identify the key antigens seen by these T cells. We have recently identified a set of in vivo expressed Mtb genes (IVE-TB which is expressed during in vivo pulmonary infection in mice, and shown that their encoded antigens are potently recognized by polyclonal T cells from tuberculin skin test-positive, in vitro ESAT-6/CFP10-responsive individuals. Here we have cloned T cells specific for one of these newly identified in vivo expressed Mtb (IVE-TB antigens, Rv2034. T cells were enriched based on the expression of CD154 (CD40L, which represents a new method for selecting antigen-specific (low frequency T cells independent of their specific function. An Rv2034-specific CD4+ T-cell clone expressed the Th1 markers T-bet, IFN-γ, TNF-α, IL-2 and the cytotoxicity related markers granzyme B and CD107a as measured by flow cytometry. The clone specifically recognized Rv2034 protein, Rv2034 peptide p81-100 and Mtb lysate. Remarkably, while the recognition of the dominant p81-100 epitope was HLA-DR restricted, the T-cell clone also recognized a neighboring epitope (p88-107 in an HLA-DR- as well as HLA-DQ1-restricted fashion. Importantly, the T-cell clone was able to inhibit Mtb outgrowth from infected monocytes significantly. The characterization of the polyfunctional and Mtb inhibitory T-cell response to IVE-TB Rv2034 at the clonal level provides detailed further insights into the potential of IVE-TB antigens as new vaccine candidate antigens in TB. Our new approach allowed the identification of T-cell subsets that likely play a significant role in controlling Mtb infection, and can be applied to the analysis of T-cell responses in patient populations.

  13. The Business Engineering Surgical Technologies (BEST) teaching method: incubating talents for surgical innovation.

    Science.gov (United States)

    de Ruijter, V; Halvax, P; Dallemagne, B; Swanström, L; Marescaux, J; Perretta, S

    2015-01-01

    Technological innovation in surgical science and healthcare is vital and calls for close collaboration between engineering and surgery. To meet this objective, BEST was designed as a free sustainable innovative teaching method for young professionals, combining surgery, engineering, and business in a multidisciplinary, high-quality, low-cost, and learning-by-doing philosophy. This paper reviews the initial outcomes of the program and discusses lessons learned and future directions of this innovative educational method. BEST educational method is delivered in two parts: the first component consisting of live streaming or pre-recorded online lectures, with an interdisciplinary profile focused on surgery, engineering, and business. The second component is an annual 5-day on-site course, organized at IRCAD-IHU, France. The program includes workshops in engineering, entrepreneurship team projects, and in-depth hands-on experience in laparoscopy, robotic surgery, interventional radiology, and flexible endoscopy with special emphasis on the interdisciplinary aspect of the training. A panel of surgeons, engineers, well-established entrepreneurs, and scientists assessed the team projects for potential patent application. From November 2011 till September 2013, 803 individual and institutional users from 79 different countries attended the online course. In total, 134 young professionals from 32 different countries applied to the onsite course. Sixty participants were selected each year for the onsite course. In addition, five participants were selected for a web-based team. Thirteen provisional patents were filed for the most promising projects. BEST proved to be a global talent incubator connecting students to high-quality education despite institutional and economical boundaries. Viable and innovative ideas arose from this revolutionary approach which is likely to spin-off significant technology transfer and lead the way for future interdisciplinary hybrid surgical

  14. Innovative Calibration Method for System Level Simulation Models of Internal Combustion Engines

    Directory of Open Access Journals (Sweden)

    Ivo Prah

    2016-09-01

    Full Text Available The paper outlines a procedure for the computer-controlled calibration of the combined zero-dimensional (0D and one-dimensional (1D thermodynamic simulation model of a turbocharged internal combustion engine (ICE. The main purpose of the calibration is to determine input parameters of the simulation model in such a way as to achieve the smallest difference between the results of the measurements and the results of the numerical simulations with minimum consumption of the computing time. An innovative calibration methodology is based on a novel interaction between optimization methods and physically based methods of the selected ICE sub-systems. Therein physically based methods were used for steering the division of the integral ICE to several sub-models and for determining parameters of selected components considering their governing equations. Innovative multistage interaction between optimization methods and physically based methods allows, unlike the use of well-established methods that rely only on the optimization techniques, for successful calibration of a large number of input parameters with low time consumption. Therefore, the proposed method is suitable for efficient calibration of simulation models of advanced ICEs.

  15. The Clone Factory

    Science.gov (United States)

    Stoddard, Beryl

    2005-01-01

    Have humans been cloned? Is it possible? Immediate interest is sparked when students are asked these questions. In response to their curiosity, the clone factory activity was developed to help them understand the process of cloning. In this activity, students reenact the cloning process, in a very simplified simulation. After completing the…

  16. Innovative method by design-around concepts with integrating the algorithm for inventive problem solving

    International Nuclear Information System (INIS)

    Chen, Wang Chih; Chen Jahau Lewis

    2014-01-01

    The work proposes a new design tool that integrates design-around concepts with the algorithm for inventive problem solving (Russian acronym: ARIZ). ARIZ includes a complete procedure for analyzing problems and related resource, resolving conflicts and generating solutions. The combination of ARIZ and design-around concepts and understanding identified principles that govern patent infringements can prevent patent infringements whenever designers innovate, greatly reducing the cost and time associated with the product design stage. The presented tool is developed from an engineering perspective rather than a legal perspective, and so can help designers easily to prevent patent infringements and succeed in innovating by designing around. An example is used to demonstrate the proposed method.

  17. METHODICS, SOFTWARE AND LABORATORY EQUIPMENT FOR AN INNOVATIVE ELECTRICAL ENGINEERING DISCIPLINE

    Directory of Open Access Journals (Sweden)

    V.S. Petrushin

    2016-09-01

    Full Text Available Purpose. Development of innovative electrical engineering discipline «Electric Machines in Mechatronic Systems» in order to improve the training of specialists of electrical engineering specialty. Methodology. The proposed project concerns the educational reforms that promote the intensification of the educational process. Results. The structure of interactive educational and training complex, which is a computer learning tool in the form of software and methodical support, as well as data and knowledge bases and consists of functionally related multimedia learning systems, interactive learning, automated control of the learning process. Originality. To offer online training and research facilities, guidelines for laboratory and computational and graphic works. Practical value. Increase the knowledge of students of educational material related to the discipline of innovation «Electric Machines in Mechatronic Systems».

  18. Innovative use of technologies and methods to redesign care: the problem of care transitions.

    Science.gov (United States)

    Richman, Mark; Sklaroff, Laura Myerchin; Hoang, Khathy; Wasson, Elijah; Gross-Schulman, Sandra

    2014-01-01

    Organizations are redesigning models of care in today's rapidly changing health care environment. Using proven innovation techniques maximizes likelihood of effective change. Our safety-net hospital aims to reduce high emergency department visit, admission, and readmission rates, key components to health care cost control. Twenty-five clinical stakeholders participated in mixed-methods innovation exercises to understand stakeholders, frame problems, and explore solutions. We identified existing barriers and means to improve post-emergency department/post-inpatient discharge care coordination/communication among patient-centered medical home care team members, including patients. Physicians and staff preferred automated e-mail notifications, including patient identifiers, medical home/primary care provider information, and relevant clinical documentation, to improve communication efficiency/efficacy.

  19. An Innovative SIFT-Based Method for Rigid Video Object Recognition

    Directory of Open Access Journals (Sweden)

    Jie Yu

    2014-01-01

    Full Text Available This paper presents an innovative SIFT-based method for rigid video object recognition (hereafter called RVO-SIFT. Just like what happens in the vision system of human being, this method makes the object recognition and feature updating process organically unify together, using both trajectory and feature matching, and thereby it can learn new features not only in the training stage but also in the recognition stage, which can improve greatly the completeness of the video object’s features automatically and, in turn, increases the ratio of correct recognition drastically. The experimental results on real video sequences demonstrate its surprising robustness and efficiency.

  20. Cloning of observables

    International Nuclear Information System (INIS)

    Ferraro, Alessandro; Galbiati, Matteo; Paris, Matteo G A

    2006-01-01

    We introduce the concept of cloning for classes of observables and classify cloning machines for qubit systems according to the number of parameters needed to describe the class under investigation. A no-cloning theorem for observables is derived and the connections between cloning of observables and joint measurements of noncommuting observables are elucidated. Relationships with cloning of states and non-demolition measurements are also analysed. (letter to the editor)

  1. Cloning of observables

    OpenAIRE

    Ferraro, Alessandro; Galbiati, Matteo; Paris, Matteo G. A.

    2005-01-01

    We introduce the concept of cloning for classes of observables and classify cloning machines for qubit systems according to the number of parameters needed to describe the class under investigation. A no-cloning theorem for observables is derived and the connections between cloning of observables and joint measurements of noncommuting observables are elucidated. Relationships with cloning of states and non-demolition measurements are also analyzed.

  2. Method-centered digital communities on protocols.io for fast-paced scientific innovation.

    Science.gov (United States)

    Kindler, Lori; Stoliartchouk, Alexei; Teytelman, Leonid; Hurwitz, Bonnie L

    2016-01-01

    The Internet has enabled online social interaction for scientists beyond physical meetings and conferences. Yet despite these innovations in communication, dissemination of methods is often relegated to just academic publishing. Further, these methods remain static, with subsequent advances published elsewhere and unlinked. For communities undergoing fast-paced innovation, researchers need new capabilities to share, obtain feedback, and publish methods at the forefront of scientific development. For example, a renaissance in virology is now underway given the new metagenomic methods to sequence viral DNA directly from an environment. Metagenomics makes it possible to "see" natural viral communities that could not be previously studied through culturing methods. Yet, the knowledge of specialized techniques for the production and analysis of viral metagenomes remains in a subset of labs.  This problem is common to any community using and developing emerging technologies and techniques. We developed new capabilities to create virtual communities in protocols.io, an open access platform, for disseminating protocols and knowledge at the forefront of scientific development. To demonstrate these capabilities, we present a virology community forum called VERVENet. These new features allow virology researchers to share protocols and their annotations and optimizations, connect with the broader virtual community to share knowledge, job postings, conference announcements through a common online forum, and discover the current literature through personalized recommendations to promote discussion of cutting edge research. Virtual communities in protocols.io enhance a researcher's ability to: discuss and share protocols, connect with fellow community members, and learn about new and innovative research in the field.  The web-based software for developing virtual communities is free to use on protocols.io. Data are available through public APIs at protocols.io.

  3. Maximizing Product Innovation through Adaptive Application of User-Centered Methods for Defining Customer Value

    Directory of Open Access Journals (Sweden)

    Tonje Overvik Olsen

    2011-12-01

    Full Text Available Establishing deep understanding of customers is a prerequisite to improve success rates of innovations under today’s transient business conditions. This paper summarizes methods and tools to increase customer understanding in new product development. A case study of developing an office chair was conducted, utilizing four such methods (web based survey, interview, observation and workshops to provide directions for later application. Results indicate that methods revealing emotionally-related customer information (workshops and observation are resource-intensive and provide less amount of information directly applicable to the product development team. The opposite is the case for methods providing more functionally-related information (web based survey and interview. The overall conclusion is that the latter methods are more suitable for product improvements, while the former may provide valuable information for creating more differentiated products.

  4. Building a competitive regional innovation environment : the regional development platform method as a tool for regional innovation policy

    OpenAIRE

    Harmaakorpi, Vesa

    2004-01-01

    The study focuses on building a regional innovation policy tool that takes into account the demands of the present techno-economic and socio-institutional paradigms. Regions are seen to be strongly dependent on their history. The competitiveness of a region is based on the regional resource configurations. In a turbulent world these resource configurations have to be renewed over time setting demands for regional dynamic capabilities. This study emphasises five regional dynamic capabilities: ...

  5. Analysis of standard and innovative methods for allocating upstream and refinery GHG emissions to oil products

    International Nuclear Information System (INIS)

    Moretti, Christian; Moro, Alberto; Edwards, Robert; Rocco, Matteo Vincenzo; Colombo, Emanuela

    2017-01-01

    Highlights: •Traditional and innovative methods for allocating emissions at refinery level are reviewed. •Added value has been introduced as a novel allocation method. •Hydrogen-based consistency test has been introduced to validate the allocation methods. •Consistent allocation methods assign negative refinery emissions to heavy products. -- Abstract: Alternative fuel policies need accurate and transparent methods to find the embedded carbon intensity of individual refinery products. This study investigates different ways of allocating greenhouse gases emissions deriving from refining and upstream crude oil supply. Allocation methods based on mass, energy content, economic value and, innovatively, added-value, are compared with the marginal refining emissions calculated by CONCAWE’s linear-programming model to the average EU refinery, which has been adopted as reference in EU legislation. Beside the most important transportation fuels (gasoline, diesel, kerosene/jet fuel and heavy fuel oil), the analysis extends to petroleum coke and refinery hydrogen. Moreover, novel criteria, based on the implications due to hydrogen usage by each fuel pathway, have been introduced to test the consistency of the analyzed approaches. It is found that only two economic-based allocation methods are consistent with the introduced criteria. These two methods also give negative refinery emissions for heavy products, which is coherent with the marginal emissions calculated through the CONCAWE refinery model. The recommended allocation methods are transparent and use only publicly available statistical data, so they may be useful not only for future EU legislation, but also in jurisdictions where a representative refinery model is not available.

  6. Innovation of floating time domain electromagnetic method in the case of environmental geophysics

    Science.gov (United States)

    Nurjanah, Siti; Widodo

    2017-07-01

    Geophysics has some methods that can be used to reveal the subsurface structure of the earth. The physical features obtained from the acquisition then analyzed and interpreted, so that it can be a great lead to interpret the physical contents, determine its position and its distribution. Geophysical methods also can be used to help the environment contamination survey which is referred to environmental geophysics. There are many sources of pollution that can harm the nature, for example, the source in the form of solid waste, liquid waste containing heavy metals, or radioactive, and etc. As time passes, these sources might settle in any sedimentary area and become sediments. Time Domain Electromagnetic (TDEM) is a trustworthy method to detect the presence of conductive anomaly due to sediment accumulation. Innovation of floating TDEM created to maximize the potential of the method, so that it can be used in aquatic environments. The configuration of TDEM modified using pipes and tires during the process of measurements. We conducted numerical simulation using Marquardt and Occam Algorithms towards synthetic model to ensure the capability of the proposed design. The development of this innovation is expected to be very useful to repair the natural conditions, especially in the water.

  7. Laser-induced micro-thermography - an innovative method of nondestructive testing of coatings. Laserinduzierte Mikrothermographie - ein innovatives ZfP-Verfahren zur Pruefung von Beschichtungen

    Energy Technology Data Exchange (ETDEWEB)

    Radtke, U; Crostack, H A; Peppler, P; Winschuh, E

    1992-01-01

    The described, innovative NDT method of the laser-induced thermowave analysis, also called laser-induced microthermography, permits the 3D imaging of thermal characteristics with high axial and lateral resolution. The results show that the method is suitable for the quality control of coatings and yields information on thickness, thermal homogeneity and adhesion as well as valuable additional information, as compared to conventional metallography. (orig.)

  8. Validation of innovative technologies and strategies for regulatory safety assessment methods: challenges and opportunities.

    Science.gov (United States)

    Stokes, William S; Wind, Marilyn

    2010-01-01

    Advances in science and innovative technologies are providing new opportunities to develop test methods and strategies that may improve safety assessments and reduce animal use for safety testing. These include high throughput screening and other approaches that can rapidly measure or predict various molecular, genetic, and cellular perturbations caused by test substances. Integrated testing and decision strategies that consider multiple types of information and data are also being developed. Prior to their use for regulatory decision-making, new methods and strategies must undergo appropriate validation studies to determine the extent that their use can provide equivalent or improved protection compared to existing methods and to determine the extent that reproducible results can be obtained in different laboratories. Comprehensive and optimal validation study designs are expected to expedite the validation and regulatory acceptance of new test methods and strategies that will support improved safety assessments and reduced animal use for regulatory testing.

  9. Design science research methods and patterns innovating information and communication technology

    CERN Document Server

    Vaishnavi, Vijay K

    2015-01-01

    Presenting innovative research methods, this second edition of a bestseller describes a simple and practical methodology for conducting cutting-edge design science research (DSR). It provides comprehensive guidance on how to conduct such research and supplies in-depth treatment of design science theory and the different types of theory that can be generated in design science research.Making novel use of the concept of patterns, it presents 84 research patterns for conducting effective DSR. It emphasizes design science theory throughout and is filled with practical examples of using patterns to

  10. Comparison of Unsupervised Vegetation Classification Methods from Vhr Images after Shadows Removal by Innovative Algorithms

    Science.gov (United States)

    Movia, A.; Beinat, A.; Crosilla, F.

    2015-04-01

    The recognition of vegetation by the analysis of very high resolution (VHR) aerial images provides meaningful information about environmental features; nevertheless, VHR images frequently contain shadows that generate significant problems for the classification of the image components and for the extraction of the needed information. The aim of this research is to classify, from VHR aerial images, vegetation involved in the balance process of the environmental biochemical cycle, and to discriminate it with respect to urban and agricultural features. Three classification algorithms have been experimented in order to better recognize vegetation, and compared to NDVI index; unfortunately all these methods are conditioned by the presence of shadows on the images. Literature presents several algorithms to detect and remove shadows in the scene: most of them are based on the RGB to HSI transformations. In this work some of them have been implemented and compared with one based on RGB bands. Successively, in order to remove shadows and restore brightness on the images, some innovative algorithms, based on Procrustes theory, have been implemented and applied. Among these, we evaluate the capability of the so called "not-centered oblique Procrustes" and "anisotropic Procrustes" methods to efficiently restore brightness with respect to a linear correlation correction based on the Cholesky decomposition. Some experimental results obtained by different classification methods after shadows removal carried out with the innovative algorithms are presented and discussed.

  11. Multi-objective genetic algorithm based innovative wind farm layout optimization method

    International Nuclear Information System (INIS)

    Chen, Ying; Li, Hua; He, Bang; Wang, Pengcheng; Jin, Kai

    2015-01-01

    Highlights: • Innovative optimization procedures for both regular and irregular shape wind farm. • Using real wind condition and commercial wind turbine parameters. • Using multiple-objective genetic algorithm optimization method. • Optimize the selection of different wind turbine types and their hub heights. - Abstract: Layout optimization has become one of the critical approaches to increase power output and decrease total cost of a wind farm. Previous researches have applied intelligent algorithms to optimizing the wind farm layout. However, those wind conditions used in most of previous research are simplified and not accurate enough to match the real world wind conditions. In this paper, the authors propose an innovative optimization method based on multi-objective genetic algorithm, and test it with real wind condition and commercial wind turbine parameters. Four case studies are conducted to investigate the number of wind turbines needed in the given wind farm. Different cost models are also considered in the case studies. The results clearly demonstrate that the new method is able to optimize the layout of a given wind farm with real commercial data and wind conditions in both regular and irregular shapes, and achieve a better result by selecting different type and hub height wind turbines.

  12. Research with School Students: Four Innovative Methods Used to Explore Effective Teaching

    Directory of Open Access Journals (Sweden)

    Jessica Faye Heal

    2015-03-01

    Full Text Available This article outlines four research methods we’ve employed to enhance how students from low-income backgrounds engage in research exploring effective teaching. It firstly outlines the need to be innovative, drawing Article 12 of the United Nations Convention on the Rights of the Child, and then moves on to explain two methods that scaffold a semi-structured interview, one child-led classroom tour and finally a creative ‘draw and tell’ approach. It argues that these methods are successful because they disrupt the researcher-participant power imbalance using the following techniques: Familiarity to the student, empowering the student to be an expert, and giving the student choice.

  13. “Rational, Emotional, Affective Learning” and the Use of Innovative Methods in Foreign Language Teaching

    Directory of Open Access Journals (Sweden)

    Paolo Frascà

    2016-05-01

    Full Text Available Foreign language didactics is a field which, notwithstanding the many centuries of application and development, is still very methodologically problematic. Statistics clearly show that, in certain countries, the educational system provides foreign language instruction that is not apt and does not deliver effective linguistic competence; a few methods, however, have been developed to contravene this problem. In the specific case of Italy, a country which is renowned for its general monolingualism, Associazione Culturale Linguistica Educational (ACLE has developed an innovative language teaching method (Rational, Emotional, Affective Learning to attempt to fill the lacunae of the Italian school system, especially in regards to the teaching of English as a foreign language. This paper seeks to briefly outline the REAL method and its applications, describe its affinity to didactic and cognitive theories, and speculate on its potential effectiveness.

  14. A Simplified Method for Gene Knockout and Direct Screening of Recombinant Clones for Application in Paenibacillus polymyxa.

    Directory of Open Access Journals (Sweden)

    Seong-Bin Kim

    Full Text Available Paenibacillus polymyxa is a bacterium widely used in agriculture, industry, and environmental remediation because it has multiple functions including nitrogen fixation and produces various biologically active compounds. Among these compounds are the antibiotics polymyxins, and the bacterium is currently being reassessed for medical application. However, a lack of genetic tools for manipulation of P. polymyxa has limited our understanding of the biosynthesis of these compounds.To facilitate an understanding of the genetic determinants of the bacterium, we have developed a system for marker exchange mutagenesis directly on competent cells of P. polymyxa under conditions where homologous recombination is enhanced by denaturation of the suicide plasmid DNA. To test this system, we targeted P. polymyxa α-and β-amylase genes for disruption. Chloramphenicol or erythromycin resistance genes were inserted into the suicide plasmid pGEM7Z-f+ (Promega. To mediate homologous recombination and replacement of the targeted genes with the antibiotic resistance genes nucleotide sequences of the α-and β-amylase genes were cloned into the plasmid flanking the antibiotic resistance genes.We have created a simple system for targeted gene deletion in P. polymyxa E681. We propose that P. polymyxa isogenic mutants could be developed using this system of marker exchange mutagenesis. α-and β-amylase genes provide a useful tool for direct recombinant screening in P. polymyxa.

  15. The methods café: An innovative idea for methods teaching at conference meetings

    NARCIS (Netherlands)

    Yanow, D.; Schwartz-Shea, P.

    2007-01-01

    Interpretive research methods of various sorts have long been used to study "the political," but the full range of such methods is not widely known, and many are curious about what they entail. Others, who begin to use one or another of them, have questions about how to proceed. For those just

  16. Multipartite asymmetric quantum cloning

    International Nuclear Information System (INIS)

    Iblisdir, S.; Gisin, N.; Acin, A.; Cerf, N.J.; Filip, R.; Fiurasek, J.

    2005-01-01

    We investigate the optimal distribution of quantum information over multipartite systems in asymmetric settings. We introduce cloning transformations that take N identical replicas of a pure state in any dimension as input and yield a collection of clones with nonidentical fidelities. As an example, if the clones are partitioned into a set of M A clones with fidelity F A and another set of M B clones with fidelity F B , the trade-off between these fidelities is analyzed, and particular cases of optimal N→M A +M B cloning machines are exhibited. We also present an optimal 1→1+1+1 cloning machine, which is an example of a tripartite fully asymmetric cloner. Finally, it is shown how these cloning machines can be optically realized

  17. A mixed methods study of emotional exhaustion: Energizing and depleting work within an innovative healthcare team.

    Science.gov (United States)

    Cain, Cindy L; Taborda-Whitt, Caitlin; Frazer, Monica; Schellinger, Sandra; White, Katie M; Kaasovic, Jason; Nelson, Brenda; Chant, Allison

    2017-11-01

    This mixed methods study documents emotional exhaustion experiences among care team members during the development of an innovative team approach for caring for adults with serious illness. A mixed methods study design was employed to examine depleting work experiences that may produce emotional exhaustion, and energizing aspects of the work that may increase meaningfulness of work, thus reducing emotional exhaustion. The population studied included team members involved in care for adults with serious illness (n = 18). Team members were surveyed quarterly over an 18-month period using the Maslach Burnout Inventory (MBI). The MBI measures burnout, defined as the inability to continue work because of the interactional toll of the work. Analyses of MBI data show that although overall levels of burnout are low, 89% of team members reported moderate/high levels of emotional exhaustion during at least one survey period. In order to understand the kinds of work experiences that may produce or ameliorate emotional exhaustion, qualitative interviews were also conducted with team members at the end of the 18-month period. Major qualitative findings indicate that disputes within the team, environmental pressures, and standardisation of meaningful work leave team members feeling depleted. Having authentic relationships with patients, working as a team, believing in the care model, and practicing autonomy and creativity help team members to restore their emotional energy. Supports for team members' well-being are critical for continued innovation. We conclude with recommendations for improving team members' well-being.

  18. Expert evaluation of innovation projects of mining enterprises on the basis of methods of system analysis and fuzzy logics

    Directory of Open Access Journals (Sweden)

    Pimonov Alexander

    2017-01-01

    Full Text Available This paper presents the multipurpose approach to evaluation of research and innovation projects based on the method of analysis of hierarchies and fuzzy logics for the mining industry. The approach, implemented as part of a decision support system, can reduce the degree of subjectivity during examinations by taking into account both quantitative and qualitative characteristics of the compared innovative alternatives; it does not depend on specific conditions of examination and allows engagement of experts of various fields of knowledge. The system includes the mechanism of coordination of several experts’ views. Using of fuzzy logics allows evaluating the qualitative characteristics of innovations in the form of formalized logical conclusions.

  19. Quantum cloning and signaling

    International Nuclear Information System (INIS)

    Simon, C.; Weihs, G.; Zeilinger, A.

    1999-01-01

    We discuss the close connections between cloning of quantum states and superluminal signaling. We present an optimal universal cloning machine based on stimulated emission recently proposed by the authors. As an instructive example, we show how a scheme for superluminal communication based on this cloning machine fails. (Authors)

  20. A New Method of Creating Technology/Function Matrix for Systematic Innovation without Expert

    Directory of Open Access Journals (Sweden)

    Tien-Yuan Cheng

    2012-02-01

    Full Text Available The technology/function matrix is comprised by specific technologies and functions, and through the technology/function matrix we can known what the technologies with functions have opportunities for innovation of product or technology. However, the technology/function matrix is very difficult to create, because the patents need to be read, analyzed and categorized into the technology/function matrix always more than hundreds or thousands. In this research, I propose a method to create a technology/function matrix just need to execute patent search without reading and analyzing patents. Through the proposed method anyone can create a technology/function matrix in a short time without experts’ help even if there are thousands of thousands of patents need to be read and analyzed.

  1. The method innovation in nuclear equipment quality witness tracing and management

    International Nuclear Information System (INIS)

    Hao Guang

    2012-01-01

    The total construction cost of a nuclear power plant, equipment procurement cost accounts for about 47%-53%. Whether the quality of equipment can meet the technical requirements plays a significant role in the operation and maintenance of a nuclear power plant. Only if we adopt effective management can the equipment quality be ensured. As the most important method of quality control, the effective attendance and track of quality witness points has a crucial effect on contract smooth implementation as well. The essay mainly illustrates the method of quality witness point tracing and management and how to incorporate serious minded ideas and all take part in ways into quality management, hoping to offer some enlightenment on the innovation of nuclear power equipment quality management. (author)

  2. Handbook of design research methods in education innovations in science, technology, engineering, and mathematics learning and teaching

    CERN Document Server

    Lesh, Richard A; Baek, John Y

    2008-01-01

    This Handbook presents the latest thinking and current examples of design research in education. Design-based research involves introducing innovations into real-world practices (as opposed to constrained laboratory contexts) and examining the impact of those designs on the learning process. Designed prototype applications (e.g., instructional methods, software or materials) and the research findings are then cycled back into the next iteration of the design innovation in order to build evidence of the particular theories being researched, and to positively impact practice and the diffusion of the innovation. The Handbook of Design Research Methods in Education-- the defining book for the field -- fills a need in how to conduct design research by those doing so right now. The chapters represent a broad array of interpretations and examples of how today's design researchers conceptualize this emergent methodology across areas as diverse as educational leadership, diffusion of innovations, complexity theory, an...

  3. Estimating the Impacts of Local Policy Innovation: The Synthetic Control Method Applied to Tropical Deforestation.

    Science.gov (United States)

    Sills, Erin O; Herrera, Diego; Kirkpatrick, A Justin; Brandão, Amintas; Dickson, Rebecca; Hall, Simon; Pattanayak, Subhrendu; Shoch, David; Vedoveto, Mariana; Young, Luisa; Pfaff, Alexander

    2015-01-01

    Quasi-experimental methods increasingly are used to evaluate the impacts of conservation interventions by generating credible estimates of counterfactual baselines. These methods generally require large samples for statistical comparisons, presenting a challenge for evaluating innovative policies implemented within a few pioneering jurisdictions. Single jurisdictions often are studied using comparative methods, which rely on analysts' selection of best case comparisons. The synthetic control method (SCM) offers one systematic and transparent way to select cases for comparison, from a sizeable pool, by focusing upon similarity in outcomes before the intervention. We explain SCM, then apply it to one local initiative to limit deforestation in the Brazilian Amazon. The municipality of Paragominas launched a multi-pronged local initiative in 2008 to maintain low deforestation while restoring economic production. This was a response to having been placed, due to high deforestation, on a federal "blacklist" that increased enforcement of forest regulations and restricted access to credit and output markets. The local initiative included mapping and monitoring of rural land plus promotion of economic alternatives compatible with low deforestation. The key motivation for the program may have been to reduce the costs of blacklisting. However its stated purpose was to limit deforestation, and thus we apply SCM to estimate what deforestation would have been in a (counterfactual) scenario of no local initiative. We obtain a plausible estimate, in that deforestation patterns before the intervention were similar in Paragominas and the synthetic control, which suggests that after several years, the initiative did lower deforestation (significantly below the synthetic control in 2012). This demonstrates that SCM can yield helpful land-use counterfactuals for single units, with opportunities to integrate local and expert knowledge and to test innovations and permutations on policies

  4. Estimating the Impacts of Local Policy Innovation: The Synthetic Control Method Applied to Tropical Deforestation

    Science.gov (United States)

    Sills, Erin O.; Herrera, Diego; Kirkpatrick, A. Justin; Brandão, Amintas; Dickson, Rebecca; Hall, Simon; Pattanayak, Subhrendu; Shoch, David; Vedoveto, Mariana; Young, Luisa; Pfaff, Alexander

    2015-01-01

    Quasi-experimental methods increasingly are used to evaluate the impacts of conservation interventions by generating credible estimates of counterfactual baselines. These methods generally require large samples for statistical comparisons, presenting a challenge for evaluating innovative policies implemented within a few pioneering jurisdictions. Single jurisdictions often are studied using comparative methods, which rely on analysts’ selection of best case comparisons. The synthetic control method (SCM) offers one systematic and transparent way to select cases for comparison, from a sizeable pool, by focusing upon similarity in outcomes before the intervention. We explain SCM, then apply it to one local initiative to limit deforestation in the Brazilian Amazon. The municipality of Paragominas launched a multi-pronged local initiative in 2008 to maintain low deforestation while restoring economic production. This was a response to having been placed, due to high deforestation, on a federal “blacklist” that increased enforcement of forest regulations and restricted access to credit and output markets. The local initiative included mapping and monitoring of rural land plus promotion of economic alternatives compatible with low deforestation. The key motivation for the program may have been to reduce the costs of blacklisting. However its stated purpose was to limit deforestation, and thus we apply SCM to estimate what deforestation would have been in a (counterfactual) scenario of no local initiative. We obtain a plausible estimate, in that deforestation patterns before the intervention were similar in Paragominas and the synthetic control, which suggests that after several years, the initiative did lower deforestation (significantly below the synthetic control in 2012). This demonstrates that SCM can yield helpful land-use counterfactuals for single units, with opportunities to integrate local and expert knowledge and to test innovations and permutations on

  5. Estimating the Impacts of Local Policy Innovation: The Synthetic Control Method Applied to Tropical Deforestation.

    Directory of Open Access Journals (Sweden)

    Erin O Sills

    Full Text Available Quasi-experimental methods increasingly are used to evaluate the impacts of conservation interventions by generating credible estimates of counterfactual baselines. These methods generally require large samples for statistical comparisons, presenting a challenge for evaluating innovative policies implemented within a few pioneering jurisdictions. Single jurisdictions often are studied using comparative methods, which rely on analysts' selection of best case comparisons. The synthetic control method (SCM offers one systematic and transparent way to select cases for comparison, from a sizeable pool, by focusing upon similarity in outcomes before the intervention. We explain SCM, then apply it to one local initiative to limit deforestation in the Brazilian Amazon. The municipality of Paragominas launched a multi-pronged local initiative in 2008 to maintain low deforestation while restoring economic production. This was a response to having been placed, due to high deforestation, on a federal "blacklist" that increased enforcement of forest regulations and restricted access to credit and output markets. The local initiative included mapping and monitoring of rural land plus promotion of economic alternatives compatible with low deforestation. The key motivation for the program may have been to reduce the costs of blacklisting. However its stated purpose was to limit deforestation, and thus we apply SCM to estimate what deforestation would have been in a (counterfactual scenario of no local initiative. We obtain a plausible estimate, in that deforestation patterns before the intervention were similar in Paragominas and the synthetic control, which suggests that after several years, the initiative did lower deforestation (significantly below the synthetic control in 2012. This demonstrates that SCM can yield helpful land-use counterfactuals for single units, with opportunities to integrate local and expert knowledge and to test innovations and

  6. Mixing Methods in Organizational Ethics and Organizational Innovativeness Research : Three Approaches to Mixed Methods Analysis

    OpenAIRE

    Riivari, Elina

    2015-01-01

    This chapter discusses three categories of mixed methods analysis techniques: variableoriented, case-oriented, and process/experience-oriented. All three categories combine qualitative and quantitative approaches to research methodology. The major differences among the categories are the focus of the study, available analysis techniques and timely aspect of the study. In variable-oriented analysis, the study focus is relationships between the research phenomena. In case-oriente...

  7. [Students of today, physicians of tomorrow. Educational priorities for innovative teaching methods in medicine].

    Science.gov (United States)

    Guicciardi, Stefano; Riforgiato, Chiara; Leopardi, Eleonora

    2015-02-01

    In the past three years there has been increasing debate regarding the educational system of physicians in Italy, mainly due to the implementation of radical changes in the selection and admission criteria to medical courses. From the students' point of view, it is possible to identify different educational challenges to be faced in order to develop a more adequate learning system.First of all, it is necessary to elaborate an accurate planning of the physicians based on the needs of the Italian Healthcare System with a proportionate regulation of access to medical and specialty courses. Secondly, there is the strong need to update the traditional curricula based on a biological reductionist perspective with innovative and integrated courses that could teach new essential topics in a radically changed and globalized world, such as global health or medical ethics. Thirdly, it is crucial to introduce innovative teaching methods that could help students improve and evaluate their practical skills and participate actively in their own education, developing the attitudes and competences needed in their future profession.

  8. Innovative method of RES integration into the regional energy development scenarios

    International Nuclear Information System (INIS)

    Klevas, Valentinas; Biekša, Kestutis; Murauskaitė, Lina

    2014-01-01

    Scarcity or abundance of energy resources usually depends on physical and geographical conditions in the region. However, the energy flow in the region also depends on the efficient use of energy resources, the consumption rate of energy and the possibility to use local renewable and non-renewable energy resources. Production, distribution and the use of energy resources in the region are the challenges for central and local government, business and social service, customers and other stakeholders. Development of regional energy economy should be optimized according to the available energy flow in the region using a network system analysis method, which provides solutions for developing sustainable energy economy models. The network system analysis method enables to optimize the use of local and renewable resources at the regional level and reveals available local energy resources. An efficient use of available regional resources and the use of renewable energy sources (RES) should be the main goals for the development of regional energy system. RES can compete with traditional fossil fuel with the condition that all hidden aspects are revealed. The network system analysis method enables to indicate energy flows in the region as well as indicate pros and cons of using renewable energy technologies. - Highlights: • RES integration into the regional energy development scenarios is done. • Innovative process network system (PNS) analysis method is used. • PNS method is used to optimize the use of local and renewable resources. • Analysis of energy flow in region using PNS method is done

  9. The Method of Relative Evaluations in the Analysis of the Innovation and Scientific-Technical Activity in the National Innovation System

    Directory of Open Access Journals (Sweden)

    Melnyk Alexander G.

    2017-04-01

    Full Text Available The article suggests the methodical approach to calculation of the system of analytical indicators for complex analysis of the innovation and scientific-technical activity by defining indexes such as: implementarity of technologies; institutional fullness of the national innovation system (NIS; high technology grade of the industry branches and sectors of the NIS. Fundamentals on the interpretation of these indicators in determining the status of institutional and technological structures, as well as the investment ability of technologies in the NIS, have been formulated. An evaluation of status of the innovation and scientific-technical activity in the industry and in the sector of scientific organizations of Ukraine was carried out on the example of practical application of the methodical approach to the analysis of statistical data. A simulation of the calculation index of implementarity of technologies in terms of the hypothesis of a 10 x magnification of the number of patents for inventions was completed. The author’s own interpretation of values of the calculated indicators has been proposed.

  10. Recombination-assisted megaprimer (RAM) cloning

    Science.gov (United States)

    Mathieu, Jacques; Alvarez, Emilia; Alvarez, Pedro J.J.

    2014-01-01

    No molecular cloning technique is considered universally reliable, and many suffer from being too laborious, complex, or expensive. Restriction-free cloning is among the simplest, most rapid, and cost-effective methods, but does not always provide successful results. We modified this method to enhance its success rate through the use of exponential amplification coupled with homologous end-joining. This new method, recombination-assisted megaprimer (RAM) cloning, significantly extends the application of restriction-free cloning, and allows efficient vector construction with much less time and effort when restriction-free cloning fails to provide satisfactory results. The following modifications were made to the protocol:•Limited number of PCR cycles for both megaprimer synthesis and the cloning reaction to reduce error propagation.•Elimination of phosphorylation and ligation steps previously reported for cloning methods that used exponential amplification, through the inclusion of a reverse primer in the cloning reaction with a 20 base pair region of homology to the forward primer.•The inclusion of 1 M betaine to enhance both reaction specificity and yield. PMID:26150930

  11. Quantum cloning without external control

    International Nuclear Information System (INIS)

    Chiara, G. de; Fazio, R.; Macchiavello, C.; Montangero, S.; Palma, G.M.

    2005-01-01

    Full text: In this work we present an approach to quantum cloning with unmodulated spin networks. The cloner is realized by a proper design of the network and a choice of the coupling between the qubits. We show that in the case of phase covariant cloner the XY coupling gives the best results. In the 1 → 2 cloning we find that the value for the fidelity of the optimal cloner is achieved, and values comparable to the optimal ones in the general N → M case can be attained. If a suitable set of network symmetries are satisfied, the output fidelity of the clones does not depend on the specific choice of the graph. We show that spin network cloning is robust against the presence of static imperfections. Moreover, in the presence of noise, it outperforms the conventional approach. In this case the fidelity exceeds the corresponding value obtained by quantum gates even for a very small amount of noise. Furthermore we show how to use this method to clone qutrits and qudits. By means of the Heisenberg coupling it is also possible to implement the universal cloner although in this case the fidelity is 10 % off that of the optimal cloner. (author)

  12. [Mystery and problems of cloning].

    Science.gov (United States)

    Nikitin, V A

    2010-01-01

    The attention of investigators is attracted to the fact that, in spite of great efforts in mammalian cloning, advances that have been made in this area of research are not great, and cloned animals have developmental pathologies often incompatible with life and/or reproduction ability. It is yet not clear what technical or biological factors underlie this, and how they are connected or interact with each other, which is more realistic strategically. There is a great number of articles dealing with the influence of cloning with the nuclear transfer on genetic and epigenetic reprogramming of donor cells. At the same time we can see the practical absence of analytical investigations concerning the technology of cloning as such, its weak points, and possible sources of cellular trauma in the course of microsurgery of nuclear transfer or twinning. This article discusses step by step several nuclear transfer techniques and the methods of dividing early preimplanted embryos for twinning with the aim to reveal possible sources of cell damage during micromanipulation that may have negative influence on the development of cloned organisms. Several new author's technologies based on the study of cell biophysical characteristics are described, which allow one to avoid cellular trauma during manipulation and minimize the possibility of cell damage at any rate.

  13. User Innovation Management

    DEFF Research Database (Denmark)

    Kanstrup, Anne Marie; Bertelsen, Pernille

    User Innovation Management (UIM) is a method for fo-opereation with users in innovation projects. The UIM method emphasizes the practice of a participatorty attitude.......User Innovation Management (UIM) is a method for fo-opereation with users in innovation projects. The UIM method emphasizes the practice of a participatorty attitude....

  14. Bioelectrochemical removal of carbon dioxide (CO2): an innovative method for biogas upgrading.

    Science.gov (United States)

    Xu, Heng; Wang, Kaijun; Holmes, Dawn E

    2014-12-01

    Innovative methods for biogas upgrading based on biological/in-situ concepts have started to arouse considerable interest. Bioelectrochemical removal of CO2 for biogas upgrading was proposed here and demonstrated in both batch and continuous experiments. The in-situ biogas upgrading system seemed to perform better than the ex-situ one, but CO2 content was kept below 10% in both systems. The in-situ system's performance was further enhanced under continuous operation. Hydrogenotrophic methanogenesis and alkali production with CO2 absorption could be major contributors to biogas upgrading. Molecular studies showed that all the biocathodes associated with biogas upgrading were dominated by sequences most similar to the same hydrogenotrophic methanogen species, Methanobacterium petrolearium (97-99% sequence identity). Conclusively, bioelectrochemical removal of CO2 showed great potential for biogas upgrading. Copyright © 2014 Elsevier Ltd. All rights reserved.

  15. Android Used in The Learning Innovation Atwood Machines on Lagrange Mechanics Methods

    Directory of Open Access Journals (Sweden)

    Shabrina Shabrina

    2017-12-01

    Full Text Available Android is one of the smartphone operating system platforms that is now widely developed in learning media. Android allows the learning process to be more flexible and not oriented to be teacher center, but it allows to be student center. The Atwood machines is an experimental tool that is often used to observe mechanical laws in constantly accelerated motion which can also be described by the Lagrange mechanics methods. As an innovative and alternative learning activity, Atwood Android-based learning apps are running for two experimental variations, which are variations in load in cart and load masses that are hung. The experiment of load-carrier mass variation found that the larger load mass in the cart, the smaller the acceleration experienced by the system. Meanwhile, the experiment on the variation of the loaded mass found that the larger the loaded mass, the greater the acceleration experienced by the system.

  16. Chemical Pretreatment Methods for the Production of Cellulosic Ethanol: Technologies and Innovations

    Directory of Open Access Journals (Sweden)

    Edem Cudjoe Bensah

    2013-01-01

    Full Text Available Pretreatment of lignocellulose has received considerable research globally due to its influence on the technical, economic and environmental sustainability of cellulosic ethanol production. Some of the most promising pretreatment methods require the application of chemicals such as acids, alkali, salts, oxidants, and solvents. Thus, advances in research have enabled the development and integration of chemical-based pretreatment into proprietary ethanol production technologies in several pilot and demonstration plants globally, with potential to scale-up to commercial levels. This paper reviews known and emerging chemical pretreatment methods, highlighting recent findings and process innovations developed to offset inherent challenges via a range of interventions, notably, the combination of chemical pretreatment with other methods to improve carbohydrate preservation, reduce formation of degradation products, achieve high sugar yields at mild reaction conditions, reduce solvent loads and enzyme dose, reduce waste generation, and improve recovery of biomass components in pure forms. The use of chemicals such as ionic liquids, NMMO, and sulphite are promising once challenges in solvent recovery are overcome. For developing countries, alkali-based methods are relatively easy to deploy in decentralized, low-tech systems owing to advantages such as the requirement of simple reactors and the ease of operation.

  17. An innovative method to calibrate a spinner anemometer without the use of yaw position sensor

    Directory of Open Access Journals (Sweden)

    G. Demurtas

    2016-09-01

    Full Text Available A spinner anemometer can be used to measure the yaw misalignment and flow inclination experienced by a wind turbine. Previous calibration methods used to calibrate a spinner anemometer for flow angle measurements were based on measurements of a spinner anemometer with default settings (arbitrary values, generally k1,d  =  1 and k2,d  =  1 and a reference yaw misalignment signal measured with a yaw position sensor. The yaw position sensor is normally present in wind turbines for control purposes; however, such a signal is not always available for a spinner anemometer calibration. Therefore, an additional yaw position sensor was installed prior to the spinner anemometer calibration. An innovative method to calibrate the spinner anemometer without a yaw positions sensor was then developed. It was noted that a non-calibrated spinner anemometer that overestimates (underestimates the inflow angle will also overestimate (underestimate the wind speed when there is a yaw misalignment. The new method leverages the non-linearity of the spinner anemometer algorithm to find the calibration factor Fα by an optimization process that minimizes the dependency of the wind speed on the yaw misalignment. The new calibration method was found to be rather robust, with Fα values within ±2.7 % of the mean value for four successive tests at the same rotor position.

  18. Business Oriented Technological System Analysis (BOTSA) at Eindhoven University of Technology : an innovative learning method to foster entrepreneurship

    NARCIS (Netherlands)

    Wijnker, M.A.S.G.; van Kasteren, H.; Romijn, H.A.; Taufik, T.; Prabasari, I.; Rineksane, I.A.; Yana, R.; Widowati, R.; Putra Rosyidi, S.A.; Riyadi, S.; Harsanto, P.

    BOTSA is an innovative teaching method for students with technical background in the field of sustainable energy technologies and an interest in entrepreneurship. Two core features of this method, namely the connection between a technological analysis and a business case as well as the involvement

  19. The use of innovative teaching methods subject in training of bachelor «Standardization and metrology»

    Directory of Open Access Journals (Sweden)

    Yushchik E. V.

    2016-04-01

    Full Text Available the article considers the application of innovative teaching methods by the example of a method of collaborative learning in the laboratory practice in teaching subject «Network information technologies» to guide the preparation of 27.03.01 «Standardization and metroliya».

  20. Cloning arbuscule-related genes from mycorrhizas

    DEFF Research Database (Denmark)

    Burleigh, Stephen

    2000-01-01

    Until recently little was known about the identity of the genes expressed in the arbuscules of mycorrhizas, due in part to problems associated with cloning genes from the tissues of an obligate symbiont. However, the combination of advanced molecular techniques, innovative use of the materials...... available and fortuitous cloning has resulted in the recent identification of a number of arbuscule-related genes. This article provides a brief summary of the genes involved in arbuscule development, function and regulation, and the techniques used to study them. Molecular techniques include differential...

  1. Strategic Innovation Capacity: A Mixed Method Study on Deliberate Strategic Learning Mechanisms

    OpenAIRE

    Berghman, Liselore

    2006-01-01

    textabstractSeveral management scholars have come to propound strategic innovation as an effective means to create new and substantially superior customer value, and to combat firms’ inclination towards strategic convergence. Research on strategic innovation is however still in its infancy, tends to lack scientific rigor and has so far proven unable to provide managers with well-founded insights into the specifics of strategic innovation creation. This research therefore aims to study mechani...

  2. An innovative method for determining the diffusion coefficient of product nuclide

    Energy Technology Data Exchange (ETDEWEB)

    Chen, Chih Lung [Dept. of Nuclear Back-end Management, Taiwan Power Company, Taipei (China); Wang, Tsing Hai [Dept. Biomedical Engineering and Environment Sciences, National Tsing Hua University, Hsinchu (China)

    2017-08-15

    Diffusion is a crucial mechanism that regulates the migration of radioactive nuclides. In this study, an innovative numerical method was developed to simultaneously calculate the diffusion coefficient of both parent and, afterward, series daughter nuclides in a sequentially reactive through-diffusion model. Two constructed scenarios, a serial reaction (RN{sub 1} → RN{sub 2} → RN{sub 3}) and a parallel reaction (RN{sub 1} → RN{sub 2}A + RN{sub 2}B), were proposed and calculated for verification. First, the accuracy of the proposed three-member reaction equations was validated using several default numerical experiments. Second, by applying the validated numerical experimental concentration variation data, the as-determined diffusion coefficient of the product nuclide was observed to be identical to the default data. The results demonstrate the validity of the proposed method. The significance of the proposed numerical method will be particularly powerful in determining the diffusion coefficients of systems with extremely thin specimens, long periods of diffusion time, and parent nuclides with fast decay constants.

  3. Learning Evaluation: blending quality improvement and implementation research methods to study healthcare innovations.

    Science.gov (United States)

    Balasubramanian, Bijal A; Cohen, Deborah J; Davis, Melinda M; Gunn, Rose; Dickinson, L Miriam; Miller, William L; Crabtree, Benjamin F; Stange, Kurt C

    2015-03-10

    In healthcare change interventions, on-the-ground learning about the implementation process is often lost because of a primary focus on outcome improvements. This paper describes the Learning Evaluation, a methodological approach that blends quality improvement and implementation research methods to study healthcare innovations. Learning Evaluation is an approach to multi-organization assessment. Qualitative and quantitative data are collected to conduct real-time assessment of implementation processes while also assessing changes in context, facilitating quality improvement using run charts and audit and feedback, and generating transportable lessons. Five principles are the foundation of this approach: (1) gather data to describe changes made by healthcare organizations and how changes are implemented; (2) collect process and outcome data relevant to healthcare organizations and to the research team; (3) assess multi-level contextual factors that affect implementation, process, outcome, and transportability; (4) assist healthcare organizations in using data for continuous quality improvement; and (5) operationalize common measurement strategies to generate transportable results. Learning Evaluation principles are applied across organizations by the following: (1) establishing a detailed understanding of the baseline implementation plan; (2) identifying target populations and tracking relevant process measures; (3) collecting and analyzing real-time quantitative and qualitative data on important contextual factors; (4) synthesizing data and emerging findings and sharing with stakeholders on an ongoing basis; and (5) harmonizing and fostering learning from process and outcome data. Application to a multi-site program focused on primary care and behavioral health integration shows the feasibility and utility of Learning Evaluation for generating real-time insights into evolving implementation processes. Learning Evaluation generates systematic and rigorous cross

  4. Statement on Human Cloning

    Science.gov (United States)

    ... as our understanding of this technology advances. Support Stem Cell Research (including Research Cloning) AAAS supports stem cell research, including the use of nuclear transplantation techniques (also ...

  5. Strategic Innovation Capacity: A Mixed Method Study on Deliberate Strategic Learning Mechanisms

    NARCIS (Netherlands)

    L.A. Berghman (Liselore)

    2006-01-01

    textabstractSeveral management scholars have come to propound strategic innovation as an effective means to create new and substantially superior customer value, and to combat firms’ inclination towards strategic convergence. Research on strategic innovation is however still in its infancy, tends to

  6. The model and the planning method of volume and variety assessment of innovative products in an industrial enterprise

    Science.gov (United States)

    Anisimov, V. G.; Anisimov, E. G.; Saurenko, T. N.; Sonkin, M. A.

    2017-01-01

    In the long term, the innovative development strategy efficiency is considered as the most crucial condition for assurance of economic system competitiveness in market conditions. It determines the problem relevance of such justification strategies with regard to specific systems features and conditions of their operation. The problem solution for industrial enterprises can be based on mathematical models of supporting the decision-making on the elements of the innovative manufacturing program. An optimization model and the planning method of innovative products volume and variety are suggested. The feature of the suggested model lies in the nonlinear nature of the objective function. It allows taking into consideration the law of diminishing marginal utility. The suggested method of optimization takes into account the system features and enables the effective implementation of manufacturing capabilities in modern conditions of production organization and sales in terms of market saturation.

  7. A Simple Method for the Extraction, PCR-amplification, Cloning, and Sequencing of Pasteuria 16S rDNA from Small Numbers of Endospores.

    Science.gov (United States)

    Atibalentja, N; Noel, G R; Ciancio, A

    2004-03-01

    For many years the taxonomy of the genus Pasteuria has been marred with confusion because the bacterium could not be cultured in vitro and, therefore, descriptions were based solely on morphological, developmental, and pathological characteristics. The current study sought to devise a simple method for PCR-amplification, cloning, and sequencing of Pasteuria 16S rDNA from small numbers of endospores, with no need for prior DNA purification. Results show that DNA extracts from plain glass bead-beating of crude suspensions containing 10,000 endospores at 0.2 x 10 endospores ml(-1) were sufficient for PCR-amplification of Pasteuria 16S rDNA, when used in conjunction with specific primers. These results imply that for P. penetrans and P. nishizawae only one parasitized female of Meloidogyne spp. and Heterodera glycines, respectively, should be sufficient, and as few as eight cadavers of Belonolaimus longicaudatus with an average number of 1,250 endospores of "Candidatus Pasteuria usgae" are needed for PCR-amplification of Pasteuria 16S rDNA. The method described in this paper should facilitate the sequencing of the 16S rDNA of the many Pasteuria isolates that have been reported on nematodes and, consequently, expedite the classification of those isolates through comparative sequence analysis.

  8. Innovative sampling and extraction methods for the determination of nonsteroidal anti-inflammatory drugs in water.

    Science.gov (United States)

    Tanwar, Shivani; Di Carro, Marina; Magi, Emanuele

    2015-03-15

    Two different innovative approaches were used for the determination of nonsteroidal anti-inflammatory drugs (NSAIDs) in water: stir bar sorptive extraction (SBSE) and passive sampling, followed by electrospray ionization liquid chromatography-tandem mass spectrometry. SBSE was developed by comparing EG-Silicone and PDMS stir bars and optimizing main parameters to attain high preconcentration. Quantitative analysis was carried out by mass spectrometry in negative ionization mode and multiple reaction monitoring. The SBSE-LC-MS/MS method provided satisfactory figures of merit with LOD (7.5-71 ng L(-1)) and LOQ (22.5-213 ng L(-1)). The developed method was successfully applied to real samples collected from river water and wastewater effluents. The obtained results showed the presence of all analytes at trace levels, in a wide range of concentrations. The passive sampling approach was carried out by using Polar Organic Chemical Integrative Sampler (POCIS); samplers were deployed for 15 days in river and tap water, allowing to detect analytes at ultra-trace levels. Time-Weighted Average concentration of NSAIDs in river water was estimated in the range 0.33-0.46 ng L(-1), using the sampling rates previously obtained by means of a simple calibration system. Copyright © 2014 Elsevier B.V. All rights reserved.

  9. Social networking and young adults' drinking practices: innovative qualitative methods for health behavior research.

    Science.gov (United States)

    Lyons, Antonia C; Goodwin, Ian; McCreanor, Tim; Griffin, Christine

    2015-04-01

    Understandings of health behaviors can be enriched by using innovative qualitative research designs. We illustrate this with a project that used multiple qualitative methods to explore the confluence of young adults' drinking behaviors and social networking practices in Aotearoa, New Zealand. Participants were 18-25 year old males and females from diverse ethnic, class, and occupational backgrounds. In Stage 1, 34 friendship focus group discussions were video-recorded with 141 young adults who talked about their drinking and social networking practices. In Stage 2, 23 individual interviews were conducted using screen-capture software and video to record participants showing and discussing their Facebook pages. In Stage 3, a database of Web-based material regarding drinking and alcohol was developed and analyzed. In friendship group data, young adults co-constructed accounts of drinking practices and networking about drinking via Facebook as intensely social and pleasurable. However, this pleasure was less prominent in individual interviews, where there was greater explication of unpleasant or problematic experiences and practices. The pleasure derived from drinking and social networking practices was also differentiated by ethnicity, gender, and social class. Juxtaposing the Web-based data with participants' talk about their drinking and social media use showed the deep penetration of online alcohol marketing into young people's social worlds. Multiple qualitative methods, generating multimodal datasets, allowed valuable nuanced insights into young adults' drinking practices and social networking behaviors. This knowledge can usefully inform health policy, health promotion strategies, and targeted health interventions. (c) 2015 APA, all rights reserved).

  10. Innovative teaching methods in the professional training of nurses – simulation education

    Directory of Open Access Journals (Sweden)

    Michaela Miertová

    2013-12-01

    Full Text Available Introduction: The article is aimed to highlight usage of innovative teaching methods within simulation education in the professional training of nurses abroad and to present our experience based on passing intensive study programme at School of Nursing, Midwifery and Social Work, University of Salford (United Kingdom, UK within Intensive EU Lifelong Learning Programme (LPP Erasmus EU RADAR 2013. Methods: Implementation of simulation methods such as role-play, case studies, simulation scenarios, practical workshops and clinical skills workstation within structured ABCDE approach (AIM© Assessment and Management Tool was aimed to promote the development of theoretical knowledge and skills to recognize and manage acutely deteriorated patients. Structured SBAR approach (Acute SBAR Communication Tool was used for the training of communication and information sharing among the members of multidisciplinary health care team. OSCE approach (Objective Structured Clinical Examination was used for student’s individual formative assessment. Results: Simulation education is proved to have lots of benefits in the professional training of nurses. It is held in safe, controlled and realistic conditions (in simulation laboratories reflecting real hospital and community care environment with no risk of harming real patients accompanied by debriefing, discussion and analysis of all activities students have performed within simulated scenario. Such learning environment is supportive, challenging, constructive, motivated, engaging, skilled, flexible, inspiring and respectful. Thus the simulation education is effective, interactive, interesting, efficient and modern way of nursing education. Conclusion: Critical thinking and clinical competences of nurses are crucial for early recognition and appropriate response to acute deterioration of patient’s condition. These competences are important to ensure the provision of high quality nursing care. Methods of

  11. Novel production method of innovative antiangiogenic and antitumor small peptides in Escherichia coli

    Directory of Open Access Journals (Sweden)

    Setrerrahmane S

    2017-11-01

    Full Text Available Sarra Setrerrahmane,1 Jian Yu,1 Jingchao Hao,1,2 Heng Zheng,3 Hanmei Xu1,3 1The Engineering Research Center of Peptide Drug Discovery and Development, China Pharmaceutical University, Nanjing, Jiangsu, 2College of Pharmacy & the Provincial Key Laboratory of Natural Drug and Pharmacology, Kunming, Yunnan, 3State Key Laboratory of Natural Medicines, Ministry of Education, China Pharmaceutical University, Nanjing, Jiangsu, People’s Republic of China Background: Developing innovative drugs with potent efficacy, specificity, and high safety remains an ongoing task in antitumor therapy development. In the last few years, peptide drugs have become attractive agents in cancer therapy. HM-3, mainly with antiangiogenic effect, and AP25, with an additional antiproliferative effect, are two peptides designed in our laboratory targeting αvβ3 and α5β1 integrins, respectively. The low molecular weight of the two peptides renders their recombinant expression very difficult, and the complicated structure of AP25 makes its chemical synthesis restricted, which presents a big challenge for its development.Methods: Bifunctional peptides designed by the ligation of HM-3 and AP25, using linkers with different flexibility, were prepared using recombinant DNA technology in Escherichia coli. The fusion peptides were expressed in a modified auto-induction medium based on a mixture of glucose, glycerol, and lactose as carbon substrates and NH4+ as nitrogen source without any amino acid or other elements. Subsequently, the antiangiogenic, antiproliferative, and cell adhesion assays were conducted to evaluate the bioactivity of the two fusion peptides.Results: The peptides were successfully expressed in a soluble form without any induction, which allows the culture to reach higher cell density before protein expression occurs. Human umbilical vein endothelial cell migration assay and chick embryo chorioallantoic membrane assay showed, at low doses, a significantly

  12. Optimally cloned binary coherent states

    DEFF Research Database (Denmark)

    Mueller, C. R.; Leuchs, G.; Marquardt, Ch

    2017-01-01

    their quantum-optimal clones. We analyze the Wigner function and the cumulants of the clones, and we conclude that optimal cloning of binary coherent states requires a nonlinearity above second order. We propose several practical and near-optimal cloning schemes and compare their cloning fidelity to the optimal...

  13. A new innovative spectrophotometric method for the simultaneous determination of sofosbuvir and ledipasvir

    Science.gov (United States)

    Mansour, Fotouh R.

    2018-01-01

    A new innovative spectrophotometric method is developed to determine the concentration ratios in binary mixtures by determining the zero crossing point in the first derivative of the gross curve. This relationship can be applied if the part of the UV spectrum of substance Y, that intersects with the overlaid spectra of substance X is straight. By plotting the intersection wavelength against the concentration ratio (Cx/Cy), a straight line was obtained with a co-efficient of determination equals 0.9999. As an application, simultaneous determination of sofosbuvir and ledipasvir in their binary mixtures was performed using two methods; a direct UV spectrophotometric method for determination of ledipasvir at 333 nm, and the new "wavelength-intersection ratio" method for determination of sofosbuvir. In the wavelength-intersection ratio method, different mixtures of sofosbuvir and ledipasvir containing different concentration ratios were prepared; the zero crossing point of the first derivative curve in the range 285 to 295 nm were determined for each mixture. An absorbance shift in the intersection was obtained with the change in the concentration ratio (sofosbuvir/ledipasvir). When the concentration ratio was plotted against the intersection wavelength, a straight line was obtained with a coefficient of determination of 0.9992. The direct method was linear in the range 3 to 18 μg/mL while the wavelength-intersection ratio method was linear in the range 11-110 μg/mL. The limits of detection were determined and found to be 0.5 and 3 μg/mL for ledipasvir and sofosbuvir, respectively. The accuracy and repeatability of the two methods were tested. The mean %recovery was found to be 100.19% and 100.75% for ledipasvir and sofosbuvir, respectively. The relative standard deviation was 0.57 for ledipasvir and 1.79 for sofosbuvir. The intermediate precision was also checked, the coefficients of variation for sofosbuvir and ledipasvir measurements between days did not exceed 1.88%.

  14. Using qualitative research methods in biomedical innovation: the case of cultured red blood cells for transfusion.

    Science.gov (United States)

    Lyall, Catherine; King, Emma

    2016-05-11

    Qualitative research has a key role to play in biomedical innovation projects. This article focuses on the appropriate use of robust social science methodologies (primarily focus group studies) for identifying the public's willingness and preference for emerging medical technologies. Our study was part of the BloodPharma project (now known as the Novosang project) to deliver industrially generated red blood cells for transfusion. Previous work on blood substitutes shows that the public prefers donated human blood. However, no research has been conducted concerning attitudes to stem cell derived red blood cells. Qualitative research methods including interviews and focus groups provide the methodological context for this paper. Focus groups were used to elicit views from sub-sections of the UK population about the potential use of such cultured red blood cells. We reflect on the appropriateness of that methodology in the context of the BloodPharma project. Findings are in the form of lessons transferable to other interdisciplinary, science-led teams about what a social science dimension can bring; why qualitative research should be included; and how it can be used effectively. Qualitative data collection offers the strength of exploring ambivalence and investigating the reasons for views, but not necessarily their prevalence in wider society. The inherent value of a qualitative method, such as focus groups, therefore lies in its ability to uncover new information. This contrasts with a quantitative approach to simply 'measuring' public opinion on a topic about which participants may have little prior knowledge. We discuss a number of challenges including: appropriate roles for embedded social scientists and the intricacies of doing upstream engagement as well as some of the design issues and limitations associated with the focus group method.

  15. Innovation activity among nurses: The translation and preliminary validation of the Bricolage Mearsure - a mixed-method study

    DEFF Research Database (Denmark)

    Krontoft, Anna; Fuglsang, Lars; Kronborg, Hanne

    2017-01-01

    in healthcare. The aim of this study was therefore to provide an instrument for measuring bricolage activity among nurses, to test the measure and learn about the spread of bricolage in nursing in Denmark. We used a mixed-method design including a translation–back translation, a pre-test and a pilot test......Qualitatively, research has demonstrated the existence of bricolage among healthcare staff, i.e. solving problems on the spot by resources at hand, and its potential to lead to innovation. However, we know little about the spread of bricolage activity and its potential role for innovation...

  16. Innovation activity among nurses: The translation and preliminary validation of the Bricolage Measure – a mixed-method study

    DEFF Research Database (Denmark)

    Krontoft, Anna; Fuglsang, Lars; Kronborg, Hanne

    2018-01-01

    in healthcare. The aim of this study was therefore to provide an instrument for measuring bricolage activity among nurses, to test the measure and learn about the spread of bricolage in nursing in Denmark. We used a mixed- method design including a translation–back translation, a pre-test and a pilot test......Qualitatively, research has demonstrated the existence of bricolage among healthcare staff, i.e. solving problems on the spot by resources at hand, and its potential to lead to innovation. However, we know little about the spread of bricolage activity and its potential role for innovation...

  17. Cloning-free CRISPR

    NARCIS (Netherlands)

    Arbab, Mandana; Srinivasan, Sharanya; Hashimoto, Tatsunori; Geijsen, Niels; Sherwood, Richard I.

    2015-01-01

    We present self-cloning CRISPR/Cas9 (scCRISPR), a technology that allows for CRISPR/Cas9-mediated genomic mutation and site-specific knockin transgene creation within several hours by circumventing the need to clone a site-specific single-guide RNA (sgRNA) or knockin homology construct for each

  18. Influence of menstruation on the microbiota of healthy women's labia minora as analyzed using a 16S rRNA gene-based clone library method.

    Science.gov (United States)

    Shiraishi, Tsukasa; Fukuda, Kazumasa; Morotomi, Nobuo; Imamura, Yuri; Mishima, Junko; Imai, Shigeo; Miyazawa, Kiyoshi; Taniguchi, Hatsumi

    2011-01-01

    The aim of this study was to determine the influence of menstruation on the bacterial population of healthy Japanese women's vulvas, especially the labia minora. Labia minora swabs were obtained from 10 premenopausal, nonpregnant Japanese women at premenstruation and on day 2 of menstruation. Vaginal swabs were also obtained from 3 out of the 10 women. No significant difference was found in the average bacterial cell count between the menstruation and premenstruation samples. Molecular analysis using a 16S rRNA gene-based clone library method detected 22 genera from the labia minora swabs (total 20), with the genus Lactobacillus being predominant at both premenstruation and during menstruation in 7 out of the 10 women. Of the other 3 women, 2 showed various kinds of bacterial species, including oral and fecal bacteria, with Atopobium vaginae and Gardnerella vaginalis predominating in the remaining woman's vulva in both conditions. In total, 6 out of 10 cases (60%) showed significantly different microbiota of the labia minora between the two conditions. These results imply that menstruation may promote a distortion of the bacterial flora around the vulva, although it causes no significant increase of the bacterial count.

  19. Design Intend Solving: Dynamic Composition Method for Innovative Design Based on Virtual Cloud Manufacturing Resource Generators

    Directory of Open Access Journals (Sweden)

    Yi-Cong Gao

    2013-01-01

    Full Text Available Recently, there has been growing interest in composition of cloud manufacturing resources (CMRs. Composition of CMRs is a feasible innovation to fulfill the user request while single cloud manufacturing resource cannot satisfy the functionality required by the user. In this paper, we propose a new case-based approach for the composition of CMRs. The basic idea of the present approach is to provide a computational framework for the composition of CMRs by imitating the common design method of reviewing past designs to obtain solution concepts for a new composite cloud manufacturing resource (CCMR. A notion of virtual cloud manufacturing resource generators (VCMRGs is introduced to conceptualize and represent underlying CCMRs contained in existing CCMRs. VCMRGs are derived from previous CCMRs and serve as new conceptual building blocks for the composition of CMRs. Feasible composite CMRs are generated by combining VCMRGs using some adaptation rules. The reuse of prior CCMRs is accomplished via VCMRGs within the framework of case-based reasoning. We demonstrate that the proposed approach yields lower execution time for fulfilling user request and shows good scalability.

  20. Innovations and trends in meat consumption: an application of the Delphi method in Spain.

    Science.gov (United States)

    Chamorro, Antonio; Miranda, Francisco J; Rubio, Sergio; Valero, Víctor

    2012-12-01

    The meat sector in Spain is an important industry. However, traditional consumption is changing as a result of the country's economic crisis and the new structure of households. The objective of the present study was to identify trends in meat consumption in Spain extrapolated to 2016, and the main innovations that should be of interest to firms in the sector. The study was conducted in 2011 using the Delphi method with the participation of 26 experts. The results showed that, while the demand for meat will not vary significantly in amount, it will do so in composition, with chicken replacing beef as the meat of most importance in the shopping basket. In addition, significant growth is expected in certified meat, but the demand for organic meat will not take off. Neither will there be no significant changes in end purchase formats, but there will be a clear trend in consumers' purchasing decision criteria away from price, external appearance and origin towards quality certification and the attributes of the packaging. With respect to end purchase channels, the experts estimate that the current trend will be accentuated with increasing market share for large supermarkets and major distribution brands. Copyright © 2012 Elsevier Ltd. All rights reserved.

  1. Responsible innovation

    CERN Document Server

    De Woot, Philippe

    2015-01-01

    Economic development is rooted in disruption, not in equilibrium. And a powerful engine of economic development is innovation; but is this innovation always for the common good? The dark side of the extraordinary dynamism of innovation lies precisely in its destructive power. If simply left to market forces, it could lead to social chaos and great human suffering. To face the challenges of our time, we must create the proper climate and culture to develop strong entrepreneurial drive. But, more than ever, we must give this entrepreneurial drive its ethical and societal dimensions. Responsible innovation means a more voluntary orientation towards the great problems of the 21st century, e.g. depletion of the planet's resources, rising inequality, and new scientific developments potentially threatening freedom, democracy and human integrity. We need to transform our ceaseless creativity into real progress for humankind. In this respect, the rapid development of social innovation opens the door for new methods an...

  2. Cloning, killing, and identity.

    Science.gov (United States)

    McMahan, J

    1999-01-01

    One potentially valuable use of cloning is to provide a source of tissues or organs for transplantation. The most important objection to this use of cloning is that a human clone would be the sort of entity that it would be seriously wrong to kill. I argue that entities of the sort that you and I essentially are do not begin to exist until around the seventh month of fetal gestation. Therefore to kill a clone prior to that would not be to kill someone like you or me but would be only to prevent one of us from existing. And even after one of us begins to exist, the objections to killing it remain comparatively weak until its psychological capacities reach a certain level of maturation. These claims support the permissibility of killing a clone during the early stages of its development in order to use its organs for transplantation. PMID:10226909

  3. Teaching methods for increasing the participations of students: Innovative dynamics games Teaching methods for increasing the participations of students: Innovative dynamics games Teaching methods for increasing the participations of students: Innovative dynamics games

    Directory of Open Access Journals (Sweden)

    Ester Oliveras

    2013-09-01

    Full Text Available Purpose: This paper analyses new dynamics as teaching methodologies in the context of   the degrees adapted to the EHEA. The aim of this study is double: to assess whether there is greater involvement in seminars with these new dynamics and to test if learning also experienced changes. Design/methodology/approach: To experiment with the introduction of innovative dynamic games in an introductory course in accounting.  These new dynamics are applied during the academic year 2010-11 in the UPF. The design, implementation and evaluation of the methodology devised have followed three stages: 1 Game Design and adequate dynamic; 2 To test the games; 2 Implementation during the course. Findings: The results show that students value positively those dynamics improving their learning and creating greater involvement. Research limitations/implications: There are some contradictory results regarding the knowledge gained by the students. Another area to be explored relates to the skills that the teacher must have in order to manage this type of dynamics. Originality/value: In an introductory level of the Financial Accounting course the most common dynamics is solving exercises. Due to the nature of matter, these are closed so they not provoke discussion among students. However, you can use activities that allow greater participation, especially through dynamics or games. This paper shows that.Purpose: This paper analyses new dynamics as teaching methodologies in the context of   the degrees adapted to the EHEA. The aim of this study is double: to assess whether there is greater involvement in seminars with these new dynamics and to test if learning also experienced changes.Design/methodology/approach: To experiment with the introduction of innovative dynamic games in an introductory course in accounting.  These new dynamics are applied during the academic year 2010-11 in the UPF. The design, implementation and evaluation of the methodology devised

  4. Cloning Mice and Men: Prohibiting the Use of iPS Cells for Human Reproductive Cloning

    OpenAIRE

    Lo, Bernard; Parham, Lindsay; Alvarez-Buylla, Arturo; Cedars, Marcelle; Conklin, Bruce; Fisher, Susan; Gates, Elena; Giudice, Linda; Halme, Dina Gould; Hershon, William; Kriegstein, Arnold; Kwok, Pui-Yan; Wagner, Richard

    2010-01-01

    The use of iPSCs and tetraploid complementation for human reproductive cloning would raise profound ethical objections. Professional standards and laws that ban human reproductive cloning by somatic cell nuclear transfer should be revised to also forbid it by other methods, such as iPSCs via tetraploid complementation.

  5. Cloning mice and men: prohibiting the use of iPS cells for human reproductive cloning.

    Science.gov (United States)

    Lo, Bernard; Parham, Lindsay; Alvarez-Buylla, Arturo; Cedars, Marcelle; Conklin, Bruce; Fisher, Susan; Gates, Elena; Giudice, Linda; Halme, Dina Gould; Hershon, William; Kriegstein, Arnold; Kwok, Pui-Yan; Wagner, Richard

    2010-01-08

    The use of iPSCs and tetraploid complementation for human reproductive cloning would raise profound ethical objections. Professional standards and laws that ban human reproductive cloning by somatic cell nuclear transfer should be revised to also forbid it by other methods, such as iPSCs via tetraploid complementation. Copyright 2010 Elsevier Inc. All rights reserved.

  6. INNOVATIVE HYBRIDSIL POLY(SILOXANE IMIDE) COMPOSITE REPAIR MATERIALS AND METHODS, Phase I

    Data.gov (United States)

    National Aeronautics and Space Administration — Through the proposed STTR program, NanoSonic and Virginia Tech will create an innovative low viscosity, high Tg copolymer injection repair material and methodology...

  7. Informal Innovation

    DEFF Research Database (Denmark)

    Hartmann, Mia Rosa Koss; Hartmann, Rasmus Koss

    Informal innovation, defined as the development and putting-into-use of novel solutions by non-R&D employees without prior formal approval from or subsequent revealing to superiors, has been recurrently observed in organizational research. But even as it is increasingly recognized that R&D is not......Informal innovation, defined as the development and putting-into-use of novel solutions by non-R&D employees without prior formal approval from or subsequent revealing to superiors, has been recurrently observed in organizational research. But even as it is increasingly recognized that R......&D is not the only plausible source of innovation inside organizations, informal innovation has yet to be systematically explored or theorized. We propose a theory of informal innovation based on analyses of prior literature and mixed-method, multi-site studies of innovation at the working level of two extreme......-case organizations. We propose that informal innovation occurs as 1) employees personally experience problems that they believe are not recognized or prioritized by superiors; 2) some employees are able to develop solutions, essentially at no cost; 3) innovators face no benefits from revealing to superiors, but can...

  8. Searching for Innovations and Methods of Using the Cultural Heritage on the Example of Upper Silesia

    Science.gov (United States)

    Wagner, Tomasz

    2017-10-01

    The basic subject of this paper is historical and cultural heritage of some parts of Upper Silesia, bind by common history and similar problems at present days. The paper presents some selected historical phenomena that have influenced contemporary space, mentioned above, and contemporary issues of heritage protection in Upper Silesia. The Silesian architecture interpretation, since 1989, is strongly covered with some ideological and national ideas. The last 25 years are the next level of development which contains rapidly transformation of the space what is caused by another economical transformations. In this period, we can observe landscape transformations, liquidation of objects and historical structures, loos of regional features, spontaneous adaptation processes of objects and many methods of implementation forms of protection, and using of cultural resources. Some upheaval linked to the state borders changes, system, economy and ethnic transformation caused that former Upper Silesia border area focuses phenomena that exists in some other similar European areas which are abutments of cultures and traditions. The latest period in the history of Upper Silesia gives us time to reflect the character of changes in architecture and city planning of the area and appraisal of efficiency these practices which are connected to cultural heritage perseveration. The phenomena of the last decades are: decrement of regional features, elimination of objects, which were a key feature of the regional cultural heritage, deformation of these forms that were shaped in the history and some trials of using these elements of cultural heritage, which are widely recognized as cultural values. In this situation, it is important to seek creative solutions that will neutralize bad processes resulting from bad law and practice. The most important phenomena of temporary space is searching of innovative fields and methods and use of cultural resources. An important part of the article is

  9. An innovative method of planning and displaying flap volume in DIEP flap breast reconstructions.

    Science.gov (United States)

    Hummelink, S; Verhulst, Arico C; Maal, Thomas J J; Hoogeveen, Yvonne L; Schultze Kool, Leo J; Ulrich, Dietmar J O

    2017-07-01

    Determining the ideal volume of the harvested flap to achieve symmetry in deep inferior epigastric artery perforator (DIEP) flap breast reconstructions is complex. With preoperative imaging techniques such as 3D stereophotogrammetry and computed tomography angiography (CTA) available nowadays, we can combine information to preoperatively plan the optimal flap volume to be harvested. In this proof-of-concept, we investigated whether projection of a virtual flap planning onto the patient's abdomen using a projection method could result in harvesting the correct flap volume. In six patients (n = 9 breasts), 3D stereophotogrammetry and CTA data were combined from which a virtual flap planning was created comprising perforator locations, blood vessel trajectory and flap size. All projected perforators were verified with Doppler ultrasound. Intraoperative flap measurements were collected to validate the determined flap delineation volume. The measured breast volume using 3D stereophotogrammetry was 578 ± 127 cc; on CTA images, 527 ± 106 cc flap volumes were planned. The nine harvested flaps weighed 533 ± 109 g resulting in a planned versus harvested flap mean difference of 5 ± 27 g (flap density 1.0 g/ml). In 41 out of 42 projected perforator locations, a Doppler signal was audible. This proof-of-concept shows in small numbers that flap volumes can be included into a virtual DIEP flap planning, and transferring the virtual planning to the patient through a projection method results in harvesting approximately the same volume during surgery. In our opinion, this innovative approach is the first step in consequently achieving symmetric breast volumes in DIEP flap breast reconstructions. Copyright © 2017 British Association of Plastic, Reconstructive and Aesthetic Surgeons. Published by Elsevier Ltd. All rights reserved.

  10. Powder Injection Molding - An innovative manufacturing method for He-cooled DEMO divertor components

    International Nuclear Information System (INIS)

    Antusch, Steffen; Norajitra, Prachai; Piotter, Volker; Ritzhaupt-Kleissl, Hans-Joachim; Spatafora, Luigi

    2011-01-01

    At Karlsruhe Institute of Technology (KIT), a He-cooled divertor design for future fusion power plants has been developed. This concept is based on the use of modular cooling fingers made from tungsten and tungsten alloy, which are presently considered the most promising divertor materials to withstand the specific heat load of 10 MW/m 2 . Since a large number of the finger modules (n > 250,000) are needed for the whole reactor, developing a mass-oriented manufacturing method is indispensable. In this regard, an innovative manufacturing technology, Powder Injection Molding (PIM), has been adapted to W processing at KIT since a couple of years. This production method is deemed promising in view of large-scale production of tungsten parts with high near-net-shape precision, hence, offering an advantage of cost-saving process compared to conventional machining. The complete technological PIM process for tungsten materials and its application on manufacturing of real divertor components, including the design of a new PIM tool is outlined and, results of the examination of the finished product after heat-treatment are discussed. A binary tungsten powder feedstock with a solid load of 50 vol.% was developed and successfully tested in molding experiments. After design, simulation and manufacturing of a new PIM tool, real divertor parts are produced. After heat-treatment (pre-sintering and HIP) the successful finished samples showed a sintered density of approximately 99%, a hardness of 457 HV0.1, a grain size of approximately 5 μm and a microstructure without cracks and porosity.

  11. First in-flight results of Pleiades 1A innovative methods for optical calibration

    Science.gov (United States)

    Kubik, Philippe; Lebègue, Laurent; Fourest, Sébastien; Delvit, Jean-Marc; de Lussy, Françoise; Greslou, Daniel; Blanchet, Gwendoline

    2017-11-01

    The PLEIADES program is a space Earth Observation system led by France, under the leadership of the French Space Agency (CNES). Since it was successfully launched on December 17th, 2011, Pleiades 1A high resolution optical satellite has been thoroughly tested and validated during the commissioning phase led by CNES. The whole system has been designed to deliver submetric optical images to users whose needs were taken into account very early in the design process. This satellite opens a new era in Europe since its off-nadir viewing capability delivers a worldwide 2- days access, and its great agility will make possible to image numerous targets, strips and stereo coverage from the same orbit. Its imaging capability of more than 450 images of 20 km x 20 km per day can fulfill a broad spectrum of applications for both civilian and defence users. For an earth observing satellite with no on-board calibration source, the commissioning phase is a critical quest of wellcharacterized earth landscapes and ground patterns that have to be imaged by the camera in order to compute or fit the parameters of the viewing models. It may take a long time to get the required scenes with no cloud, whilst atmosphere corrections need simultaneous measurements that are not always possible. The paper focuses on new in-flight calibration methods that were prepared before the launch in the framework of the PLEIADES program : they take advantage of the satellite agility that can deeply relax the operational constraints and may improve calibration accuracy. Many performances of the camera were assessed thanks to a dedicated innovative method that was successfully validated during the commissioning period : Modulation Transfer Function (MTF), refocusing, absolute calibration, line of sight stability were estimated on stars and on the Moon. Detectors normalization and radiometric noise were computed on specific pictures on Earth with a dedicated guidance profile. Geometric viewing frame was

  12. Innovative method and apparatus for the deep cleaning of soluble salts from mortars and lithic materials

    Science.gov (United States)

    Gaggero, Laura; Ferretti, Maurizio; Torrielli, Giulia; Caratto, Valentina

    2016-04-01

    demonstrated by conducibility tests on the juxtaposed Japanese paper. In addition, after the conventional treatment, a considerable amount of soluble salts was further extracted demonstrating that traditional wraps operate just a shallow cleaning, and soluble salts are liable to emerge later as efflorescence affecting the conservation after restoration. The optimum cleaning was obtained by finishing the innovative extraction with sepiolite/cellulose wraps. As a whole, the novel method and apparatus enhance the time for restoration and the final quality before consolidation and protection. [1] "Apparatus and method for treating porous materials" - M. Ferretti, L. Gaggero, G. Torrielli, PCT/IB2015/055129 (2015)

  13. Evaluation of Students' Perceptions Towards An Innovative Teaching-Learning Method During Pharmacology Revision Classes: Autobiography of Drugs.

    Science.gov (United States)

    Joshi, Anuradha; Ganjiwale, Jaishree

    2015-07-01

    Various studies in medical education have shown that active learning strategies should be incorporated into the teaching-learning process to make learning more effective, efficient and meaningful. The aim of this study was to evaluate student's perceptions on an innovative revision method conducted in Pharmacology i.e. in form of Autobiography of Drugs. The main objective of study was to help students revise the core topics in Pharmacology in an interesting way. Questionnaire based survey on a newer method of pharmacology revision in two batches of second year MBBS students of a tertiary care teaching medical college. Various sessions on Autobiography of Drugs were conducted amongst two batches of second year MBBS students, during their Pharmacology revision classes. Student's perceptions were documented with the help of a five point likert scale through a questionnaire regarding quality, content and usefulness of this method. Descriptive analysis. Students of both the batches appreciated the innovative method taken up for revision. The median scores in most of the domains in both batches were four out of five, indicative of good response. Feedback from open-ended questions also revealed that the innovative module on "Autobiography of Drugs" was taken as a positive learning experience by students. Autobiography of drugs has been used to help students recall topics that they have learnt through other teachings methods. Autobiography sessions in Pharmacology during revision slots, can be one of the interesting ways in helping students revise and recall topics which have already been taught in theory classes.

  14. Unified Approach to Universal Cloning and Phase-Covariant Cloning

    OpenAIRE

    Hu, Jia-Zhong; Yu, Zong-Wen; Wang, Xiang-Bin

    2008-01-01

    We analyze the problem of approximate quantum cloning when the quantum state is between two latitudes on the Bloch's sphere. We present an analytical formula for the optimized 1-to-2 cloning. The formula unifies the universal quantum cloning (UQCM) and the phase covariant quantum cloning.

  15. Main: Clone Detail [KOME

    Lifescience Database Archive (English)

    Full Text Available Clone Detail Mapping Pseudomolecule data detail Detail information Mapping to the T...IGR japonica Pseudomolecules kome_mapping_pseudomolecule_data_detail.zip kome_mapping_pseudomolecule_data_detail ...

  16. BIOETHICS AND HUMAN CLONING

    Directory of Open Access Journals (Sweden)

    Željko Kaluđerović

    2011-12-01

    Full Text Available In this paper the authors analyze the process of negotiating and beginning of the United Nations Declaration on Human Cloning as well as the paragraphs of the very Declaration. The negotiation was originally conceived as a clear bioethical debate that should have led to a general agreement to ban human cloning. However, more often it had been discussed about human rights, cultural, civil and religious differences between people and about priorities in case of eventual conflicts between different value systems. In the end, a non-binding Declaration on Human Cloning had been adopted, full of numerous compromises and ambiguous formulations, that relativized the original intention of proposer states. According to authors, it would have been better if bioethical discussion and eventual regulations on cloning mentioned in the following text had been left over to certain professional bodies, and only after the public had been fully informed about it should relevant supranational organizations have taken that into consideration.

  17. Do Managers Clone Themselves?

    Science.gov (United States)

    Baron, Alma S.

    1981-01-01

    A recent questionnaire survey provides statistics on male managers' views of female managers. The author recommends that male managers break out of their cloning behavior and that the goal ought to be a plurality in management. (Author/WD)

  18. Participatory Innovation

    DEFF Research Database (Denmark)

    Buur, Jacob; Matthews, Ben

    2008-01-01

    An increasing number of corporations engage with users in co-innovation of products and services. But there are a number of competing perspectives on how best to integrate these understandings into existing corporate innovation development processes. This paper maps out three of the dominant appr...... the challenges such an approach sets to innovation management, and discuss research directions we see as fundamental to the development of the field of user-driven innovation. Udgivelsesdato: September......An increasing number of corporations engage with users in co-innovation of products and services. But there are a number of competing perspectives on how best to integrate these understandings into existing corporate innovation development processes. This paper maps out three of the dominant...... approaches, compares them in terms of goals, methods and basic philosophy, and shows how they may beneficially enrich one another. We will present an industrial innovation case that has been instrumental to the development of what we have termed ‘Participatory Innovation’. Based on this we will list...

  19. Proposal for a Method for Business Model Performance Assessment: Toward an Experimentation Tool for Business Model Innovation

    Directory of Open Access Journals (Sweden)

    Antonio Batocchio

    2017-04-01

    Full Text Available The representation of business models has been recently widespread, especially in the pursuit of innovation. However, defining a company’s business model is sometimes limited to discussion and debates. This study observes the need for performance measurement so that business models can be data-driven. To meet this goal, the work proposed as a hypothesis the creation of a method that combines the practices of the Balanced Scorecard with a method of business models representation – the Business Model Canvas. Such a combination was based on study of conceptual adaptation, resulting in an application roadmap. A case study application was performed to check the functionality of the proposition, focusing on startup organizations. It was concluded that based on the performance assessment of the business model it is possible to propose the search for change through experimentation, a path that can lead to business model innovation.

  20. Asymmetric quantum cloning machines

    International Nuclear Information System (INIS)

    Cerf, N.J.

    1998-01-01

    A family of asymmetric cloning machines for quantum bits and N-dimensional quantum states is introduced. These machines produce two approximate copies of a single quantum state that emerge from two distinct channels. In particular, an asymmetric Pauli cloning machine is defined that makes two imperfect copies of a quantum bit, while the overall input-to-output operation for each copy is a Pauli channel. A no-cloning inequality is derived, characterizing the impossibility of copying imposed by quantum mechanics. If p and p ' are the probabilities of the depolarizing channels associated with the two outputs, the domain in (√p,√p ' )-space located inside a particular ellipse representing close-to-perfect cloning is forbidden. This ellipse tends to a circle when copying an N-dimensional state with N→∞, which has a simple semi-classical interpretation. The symmetric Pauli cloning machines are then used to provide an upper bound on the quantum capacity of the Pauli channel of probabilities p x , p y and p z . The capacity is proven to be vanishing if (√p x , √p y , √p z ) lies outside an ellipsoid whose pole coincides with the depolarizing channel that underlies the universal cloning machine. Finally, the tradeoff between the quality of the two copies is shown to result from a complementarity akin to Heisenberg uncertainty principle. (author)

  1. Team learning for innovation in higher education : a mixed methods study

    NARCIS (Netherlands)

    Bron, Rike; Endedijk, Maaike; Sleegers, P.J.C.

    2015-01-01

    In Higher Education, many changes towards more student-centred and interdisciplinary education are designed and implemented. In order to design such educational innovations, teachers in higher education have to work together in teams. In this research we study these teacher collaboration processes

  2. Innovative methods to stabilize liquid membranes for removal of radionuclides from groundwater

    Energy Technology Data Exchange (ETDEWEB)

    Lokhandwala, K. [Membrane Technology and Research, Inc., Menlo Park, CA (United States)

    1997-10-01

    In this Phase I Small Business Innovation Research program, Membrane Technology Research, Inc., is developing a stable liquid membrane for extracting uranium and other radionuclides from groundwater. The improved membrane can also be applied to separation of other metal ions from aqueous streams in industrial operations.

  3. Sequential method for the assessment of innovations in computer assisted industrial processes; Metodo secuencial para evaluacion de innovaciones en procesos industriales asistido por computadora

    Energy Technology Data Exchange (ETDEWEB)

    Suarez Antola, R [Universidad Catolica del Uruguay, Montevideo (Uruguay); Artucio, G [Ministerio de Industria Energia y Mineria. Direccion Nacional de Tecnologia Nuclear, Montevideo (Uruguay)

    1995-08-01

    A sequential method for the assessment of innovations in industrial processes is proposed, using suitable combinations of mathematical modelling and numerical simulation of dynamics. Some advantages and limitations of the proposed method are discussed. tabs.

  4. Open innovations, innovation communities and firm's innovative activities

    Directory of Open Access Journals (Sweden)

    Getejanc Vesna

    2016-01-01

    Full Text Available The open innovation paradigm emphasizes the fact that firms can improve their performance by opening their business models and reduce their R&D costs by effective incorporation of external knowledge. In other words, companies are able to capture value through knowledge that exists outside the boundaries of their organization. The shift from closed to open model of innovation has imposed the necessity to adopt more open approach to innovation within traditional academic view of business strategy. The adoption of this innovative approach is emphasized even more, by the necessity for stronger connection and cooperation among the participants of the innovation process. Free will and collaboration are the main characteristics of open source software, which is recognized in literature as the role model of open innovation and is a rapidly growing method of technology development. Furthermore, innovative communities represent a great opportunity for improvement of the companies' innovation activities, since they have become an important source for identifying the needs and problems of the users. Their development has been fostered by information technologies and recent social changes in user behavior. Recognizing and better understanding the motivation of the members of the innovation communities that guide them to participate in the process of idea generation, can have significant influence on their incorporation within the innovation process. Equally important is to define the incentives that are suited for stimulating and fostering innovative user activities. Taking this topic in consideration, the purpose of this article is to address the following questions: In what way does the collaboration in open source software projects have positive effect on companies' innovation performance? What are the innovation communities and how can companies establish successful interaction with them? Why does the interaction with innovation communities lead to

  5. Methods for open innovation on a genome-design platform associating scientific, commercial, and educational communities in synthetic biology.

    Science.gov (United States)

    Toyoda, Tetsuro

    2011-01-01

    Synthetic biology requires both engineering efficiency and compliance with safety guidelines and ethics. Focusing on the rational construction of biological systems based on engineering principles, synthetic biology depends on a genome-design platform to explore the combinations of multiple biological components or BIO bricks for quickly producing innovative devices. This chapter explains the differences among various platform models and details a methodology for promoting open innovation within the scope of the statutory exemption of patent laws. The detailed platform adopts a centralized evaluation model (CEM), computer-aided design (CAD) bricks, and a freemium model. It is also important for the platform to support the legal aspects of copyrights as well as patent and safety guidelines because intellectual work including DNA sequences designed rationally by human intelligence is basically copyrightable. An informational platform with high traceability, transparency, auditability, and security is required for copyright proof, safety compliance, and incentive management for open innovation in synthetic biology. GenoCon, which we have organized and explained here, is a competition-styled, open-innovation method involving worldwide participants from scientific, commercial, and educational communities that aims to improve the designs of genomic sequences that confer a desired function on an organism. Using only a Web browser, a participating contributor proposes a design expressed with CAD bricks that generate a relevant DNA sequence, which is then experimentally and intensively evaluated by the GenoCon organizers. The CAD bricks that comprise programs and databases as a Semantic Web are developed, executed, shared, reused, and well stocked on the secure Semantic Web platform called the Scientists' Networking System or SciNetS/SciNeS, based on which a CEM research center for synthetic biology and open innovation should be established. Copyright © 2011 Elsevier Inc

  6. The module of methodical support in system of electronic educational resources as the innovative element of the modern maintenance of formation

    Directory of Open Access Journals (Sweden)

    Ольга Николаевна Крылова

    2009-06-01

    Full Text Available The article introduces some results of research, which were devoted to evaluation of tearches' mobility to introduce innovations in the contents of education. The author considers innovative potential of modules of the methodical support for system of electronic educational resources.

  7. Several Methods for Evaluating the Investment Attractiveness of Small Innovation Enterprises

    Directory of Open Access Journals (Sweden)

    Ignatova Iuliia V.

    2017-04-01

    Full Text Available An important factor of impact on the development and living abilities of small and medium-sized innovation enterprises, including startups, is the opportunity to evaluate their investment attractiveness. The main reason for the «failure» of such enterprises is the lack of instrumentarium to forecast the potential number of their customers, and therefore their financial results. The article suggests the number of projected customers as an indicator for evaluation of the investment attractiveness of small innovation enterprises. The authors propose to use a number of mathematical models on the basis of the instrumentarium of descriptive statistics and simulation modeling. The proposed models are built on the basis of the hypothesis of normality of the distribution law of random amounts of income clients and allow forecasting with high accuracy in relation to the day of week, and therefore evaluating the investment risks for potential investors.

  8. Exponential megapriming PCR (EMP cloning--seamless DNA insertion into any target plasmid without sequence constraints.

    Directory of Open Access Journals (Sweden)

    Alexander Ulrich

    Full Text Available We present a fast, reliable and inexpensive restriction-free cloning method for seamless DNA insertion into any plasmid without sequence limitation. Exponential megapriming PCR (EMP cloning requires two consecutive PCR steps and can be carried out in one day. We show that EMP cloning has a higher efficiency than restriction-free (RF cloning, especially for long inserts above 2.5 kb. EMP further enables simultaneous cloning of multiple inserts.

  9. Evaluation of Students’ Perceptions Towards An Innovative Teaching-Learning Method During Pharmacology Revision Classes: Autobiography of Drugs

    Science.gov (United States)

    Ganjiwale, Jaishree

    2015-01-01

    Introduction Various studies in medical education have shown that active learning strategies should be incorporated into the teaching–learning process to make learning more effective, efficient and meaningful. Objectives The aim of this study was to evaluate student’s perceptions on an innovative revision method conducted in Pharmacology i.e. in form of Autobiography of Drugs. The main objective of study was to help students revise the core topics in Pharmacology in an interesting way. Settings and Design Questionnaire based survey on a newer method of pharmacology revision in two batches of second year MBBS students of a tertiary care teaching medical college. Materials and Methods Various sessions on Autobiography of Drugs were conducted amongst two batches of second year MBBS students, during their Pharmacology revision classes. Student’s perceptions were documented with the help of a five point likert scale through a questionnaire regarding quality, content and usefulness of this method. Statistical analysis used Descriptive analysis. Results Students of both the batches appreciated the innovative method taken up for revision. The median scores in most of the domains in both batches were four out of five, indicative of good response. Feedback from open-ended questions also revealed that the innovative module on “Autobiography of Drugs” was taken as a positive learning experience by students. Conclusions Autobiography of drugs has been used to help students recall topics that they have learnt through other teachings methods. Autobiography sessions in Pharmacology during revision slots, can be one of the interesting ways in helping students revise and recall topics which have already been taught in theory classes. PMID:26393138

  10. An innovative strategy to clone positive modifier genes of defects caused by mtDNA mutations: MRPS18C as suppressor gene of m.3946G>A mutation in MT-ND1 gene.

    Science.gov (United States)

    Rodríguez-García, María Elena; Cotrina-Vinagre, Francisco Javier; Carnicero-Rodríguez, Patricia; Martínez-Azorín, Francisco

    2017-07-01

    We have developed a new functional complementation approach to clone modifier genes which overexpression is able to suppress the biochemical defects caused by mtDNA mutations (suppressor genes). This strategy consists in transferring human genes into respiratory chain-deficient fibroblasts, followed by a metabolic selection in a highly selective medium. We used a normalized expression cDNA library in an episomal vector (pREP4) to transfect the fibroblasts, and a medium with glutamine and devoid of any carbohydrate source to select metabolically. Growing the patient's fibroblasts in this selective medium, the deficient cells rapidly disappear unless they are rescued by the cDNA of a suppressor gene. The use of an episomal vector allows us to carry out several rounds of transfection/selection (cyclical phenotypic rescue) to enrich the rescue with true clones of suppressor genes. Using fibroblasts from a patient with epileptic encephalopathy with the m.3946G>A (p.E214K) mutation in the MT-ND1 gene, several candidate genes were identified and one of them was characterized functionally. Thus, overexpression of MRPS18C gene (that encode for bS18m protein) suppressed the molecular defects produced by this mtDNA mutation, recovering the complex I activity and reducing the ROS produced by this complex to normal levels. We suggest that modulation of bS18m expression may be an effective therapeutic strategy for the patients with this mutation.

  11. Method-centered digital communities on protocols.io for fast-paced scientific innovation [version 2; referees: 2 approved

    Directory of Open Access Journals (Sweden)

    Lori Kindler

    2017-06-01

    Full Text Available The Internet has enabled online social interaction for scientists beyond physical meetings and conferences. Yet despite these innovations in communication, dissemination of methods is often relegated to just academic publishing. Further, these methods remain static, with subsequent advances published elsewhere and unlinked. For communities undergoing fast-paced innovation, researchers need new capabilities to share, obtain feedback, and publish methods at the forefront of scientific development. For example, a renaissance in virology is now underway given the new metagenomic methods to sequence viral DNA directly from an environment. Metagenomics makes it possible to "see" natural viral communities that could not be previously studied through culturing methods. Yet, the knowledge of specialized techniques for the production and analysis of viral metagenomes remains in a subset of labs.  This problem is common to any community using and developing emerging technologies and techniques. We developed new capabilities to create virtual communities in protocols.io, an open access platform, for disseminating protocols and knowledge at the forefront of scientific development. To demonstrate these capabilities, we present a virology community forum called VERVENet. These new features allow virology researchers to share protocols and their annotations and optimizations, connect with the broader virtual community to share knowledge, job postings, conference announcements through a common online forum, and discover the current literature through personalized recommendations to promote discussion of cutting edge research. Virtual communities in protocols.io enhance a researcher's ability to: discuss and share protocols, connect with fellow community members, and learn about new and innovative research in the field.  The web-based software for developing virtual communities is free to use on protocols.io. Data are available through public APIs at protocols.io.

  12. An Improved Single-Step Cloning Strategy Simplifies the Agrobacterium tumefaciens-Mediated Transformation (ATMT)-Based Gene-Disruption Method for Verticillium dahliae.

    Science.gov (United States)

    Wang, Sheng; Xing, Haiying; Hua, Chenlei; Guo, Hui-Shan; Zhang, Jie

    2016-06-01

    The soilborne fungal pathogen Verticillium dahliae infects a broad range of plant species to cause severe diseases. The availability of Verticillium genome sequences has provided opportunities for large-scale investigations of individual gene function in Verticillium strains using Agrobacterium tumefaciens-mediated transformation (ATMT)-based gene-disruption strategies. Traditional ATMT vectors require multiple cloning steps and elaborate characterization procedures to achieve successful gene replacement; thus, these vectors are not suitable for high-throughput ATMT-based gene deletion. Several advancements have been made that either involve simplification of the steps required for gene-deletion vector construction or increase the efficiency of the technique for rapid recombinant characterization. However, an ATMT binary vector that is both simple and efficient is still lacking. Here, we generated a USER-ATMT dual-selection (DS) binary vector, which combines both the advantages of the USER single-step cloning technique and the efficiency of the herpes simplex virus thymidine kinase negative-selection marker. Highly efficient deletion of three different genes in V. dahliae using the USER-ATMT-DS vector enabled verification that this newly-generated vector not only facilitates the cloning process but also simplifies the subsequent identification of fungal homologous recombinants. The results suggest that the USER-ATMT-DS vector is applicable for efficient gene deletion and suitable for large-scale gene deletion in V. dahliae.

  13. Emotional reactions to human reproductive cloning.

    Science.gov (United States)

    May, Joshua

    2016-01-01

    Extant surveys of people's attitudes towards human reproductive cloning focus on moral judgements alone, not emotional reactions or sentiments. This is especially important given that some (especially Leon Kass) have argued against such cloning on the ground that it engenders widespread negative emotions, like disgust, that provide a moral guide. To provide some data on emotional reactions to human cloning, with a focus on repugnance, given its prominence in the literature. This brief mixed-method study measures the self-reported attitudes and emotions (positive or negative) towards cloning from a sample of participants in the USA. Most participants condemned cloning as immoral and said it should be illegal. The most commonly reported positive sentiment was by far interest/curiosity. Negative emotions were much more varied, but anxiety was the most common. Only about a third of participants selected disgust or repugnance as something they felt, and an even smaller portion had this emotion come to mind prior to seeing a list of options. Participants felt primarily interested and anxious about human reproductive cloning. They did not primarily feel disgust or repugnance. This provides initial empirical evidence that such a reaction is not appropriately widespread. Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://www.bmj.com/company/products-services/rights-and-licensing/

  14. Lovely clone of coconuts

    Energy Technology Data Exchange (ETDEWEB)

    Branton, R.; Blake, J.

    1983-05-01

    It has taken over 10 years research and development to clone oil palms and coconut palms successfully. Unilever has recently built a tissue culture factory in England with a potential capacity for producing half a million clonal oil palms a year for export. Research on the cloning of coconut palms is reported here. Cloned palms may increase yields from oil palms by 20 to 30 percent and yields from coconut could be as high as five-fold over unselected stock. Improved yields would not only increase the yield of oil and copra but also the harvests of husk and shell which are immense potential sources of energy; the 1978 Philippine harvest of over 12 million nuts is equivalent in terms of energy to 3.8 billion litres of petrol (31 x 10/sup 12/ kcal).

  15. A COMMUNITY-BASED COLLEGE STUDY ON ADHERENCE TO DOTS AND RELATED DIAGNOSTIC INNOVATIONS ON AFB STAINING METHOD

    Directory of Open Access Journals (Sweden)

    Nilotpal Banerjee

    2016-10-01

    Full Text Available BACKGROUND The geographically isolated underdeveloped state, Tripura of north-eastern region of India has been facing multidimensional problems like unemployment, lack of hygiene, illiteracy, lack of industrialisation, etc. So, it was intended to study the magnitude of reported tuberculosis in the community around the Tripura Medical College through RNTCP. MATERIALS AND METHODS Questionnaire, interrogative methods was primarily adopted. Inspection to different villages around and direct interrogation was done on awareness about the programme. Self-modified innovative method was also adopted to compare scientific reproducibility along with conventional method for AFB staining. RESULTS Analysis of results reveal 28% of people interrogated knew about RNTC programme. 16% of people had seen and heard about the TB staff. 56% of people said that no health staff enquired on other factors like drinking water, mosquito and hygiene, etc. The innovative method of staining proved 100% successful, which was also easier, cheaper and more rapid. CONCLUSION DMC and DOTs services had been quite good as evident from document records and on interrogation to the attendees. 80% of attendees said that they would complete the course of treatment. 83% were happy with the provisions of DOTs and DMC.

  16. High-throughput cloning and expression in recalcitrant bacteria

    NARCIS (Netherlands)

    Geertsma, Eric R.; Poolman, Bert

    We developed a generic method for high-throughput cloning in bacteria that are less amenable to conventional DNA manipulations. The method involves ligation-independent cloning in an intermediary Escherichia coli vector, which is rapidly converted via vector-backbone exchange (VBEx) into an

  17. A Seminar on Human Cloning: Cloning in Reproductive Medicine

    OpenAIRE

    Illmensee, Karl

    2001-01-01

    This review article summarizes the historical development of mammalian cloning, presents current advances and presumed risk factors in the field of reproductive cloning, discusses possible clinical applications of therapeutic and diagnostic cloning and outlines prospective commercial trends in pharmacytical cloning. Predictable progress in biotechnology and stem cell engineering should prove to be advantageous for patients' health and for novel benefits in reproductive and regenerative medicine.

  18. Synergetic Basis of Innovation Marketing

    OpenAIRE

    Melnyk, L; Dehtyarova, I

    2012-01-01

    Issues of synergetic effects of innovation marketing are considered in the article. It shows sources of synergetic effects of innovation marketing, types of synergism, synergetic effects of marketing innovations, as well as evaluation methods.

  19. High-dimensional quantum cloning and applications to quantum hacking.

    Science.gov (United States)

    Bouchard, Frédéric; Fickler, Robert; Boyd, Robert W; Karimi, Ebrahim

    2017-02-01

    Attempts at cloning a quantum system result in the introduction of imperfections in the state of the copies. This is a consequence of the no-cloning theorem, which is a fundamental law of quantum physics and the backbone of security for quantum communications. Although perfect copies are prohibited, a quantum state may be copied with maximal accuracy via various optimal cloning schemes. Optimal quantum cloning, which lies at the border of the physical limit imposed by the no-signaling theorem and the Heisenberg uncertainty principle, has been experimentally realized for low-dimensional photonic states. However, an increase in the dimensionality of quantum systems is greatly beneficial to quantum computation and communication protocols. Nonetheless, no experimental demonstration of optimal cloning machines has hitherto been shown for high-dimensional quantum systems. We perform optimal cloning of high-dimensional photonic states by means of the symmetrization method. We show the universality of our technique by conducting cloning of numerous arbitrary input states and fully characterize our cloning machine by performing quantum state tomography on cloned photons. In addition, a cloning attack on a Bennett and Brassard (BB84) quantum key distribution protocol is experimentally demonstrated to reveal the robustness of high-dimensional states in quantum cryptography.

  20. Human cloning: category, dignity, and the role of bioethics.

    Science.gov (United States)

    Shuster, Evelyne

    2003-10-01

    Human cloning has been simultaneously a running joke for massive worldwide publicity of fringe groups like the Raelians, and the core issue of an international movement at the United Nations in support of a treaty to ban the use of cloning techniques to produce a child (so called reproductive cloning). Yet, even though debates on human cloning have greatly increased since the birth of Dolly, the clone sheep, in 1997, we continue to wonder whether cloning is after all any different from other methods of medically assisted reproduction, and what exactly makes cloning an 'affront to the dignity of humans.' Categories we adopt matter mightily as they inform but can also misinform and lead to mistaken and unproductive decisions. And thus bioethicists have a responsibility to ensure that the proper categories are used in the cloning debates and denounce those who try to win the ethical debate through well-crafted labels rather than well-reasoned argumentations. But it is as important for bioethicists to take a position on broad issues such as human cloning and species altering interventions. One 'natural question' would be, for example, should there be an international treaty to ban human reproductive cloning?

  1. An innovative method for offshore wind farm site selection based on the interval number with probability distribution

    Science.gov (United States)

    Wu, Yunna; Chen, Kaifeng; Xu, Hu; Xu, Chuanbo; Zhang, Haobo; Yang, Meng

    2017-12-01

    There is insufficient research relating to offshore wind farm site selection in China. The current methods for site selection have some defects. First, information loss is caused by two aspects: the implicit assumption that the probability distribution on the interval number is uniform; and ignoring the value of decision makers' (DMs') common opinion on the criteria information evaluation. Secondly, the difference in DMs' utility function has failed to receive attention. An innovative method is proposed in this article to solve these drawbacks. First, a new form of interval number and its weighted operator are proposed to reflect the uncertainty and reduce information loss. Secondly, a new stochastic dominance degree is proposed to quantify the interval number with a probability distribution. Thirdly, a two-stage method integrating the weighted operator with stochastic dominance degree is proposed to evaluate the alternatives. Finally, a case from China proves the effectiveness of this method.

  2. Innovations (INNO)

    International Nuclear Information System (INIS)

    1978-01-01

    The annual report of the working group on innovations (INNO) deals with the projects carried out by the participating institutes and laboratories in the two fields of 1) application of non-nuclear methods and 2) non-nuclear research and development. (HK) [de

  3. Myths about Cloning

    Science.gov (United States)

    ... aging normally. In fact, the first cattle clones ever produced are alive, healthy, and are 10 years old as of January 2008. Back to the ... until we finish assessing their safety. To the best of our knowledge, they have done so. After years of detailed study and analysis, FDA has concluded ...

  4. Clip, connect, clone

    DEFF Research Database (Denmark)

    Fujima, Jun; Lunzer, Aran; Hornbæk, Kasper

    2010-01-01

    using three mechanisms: clipping of input and result elements from existing applications to form cells on a spreadsheet; connecting these cells using formulas, thus enabling result transfer between applications; and cloning cells so that multiple requests can be handled side by side. We demonstrate...

  5. [Nuclear transfer and therapeutic cloning].

    Science.gov (United States)

    Xu, Xiao-Ming; Lei, An-Min; Hua, Jin-Lian; Dou, Zhong-Ying

    2005-03-01

    Nuclear transfer and therapeutic cloning have widespread and attractive prospects in animal agriculture and biomedical applications. We reviewed that the quality of oocytes and nuclear reprogramming of somatic donor cells were the main reasons of the common abnormalities in cloned animals and the low efficiency of cloning and showed the problems and outlets in therapeutic cloning, such as some basic problems in nuclear transfer affected clinical applications of therapeutic cloning. Study on isolation and culture of nuclear transfer embryonic stem (ntES) cells and specific differentiation of ntES cells into important functional cells should be emphasized and could enhance the efficiency. Adult stem cells could help to cure some great diseases, but could not replace therapeutic cloning. Ethics also impeded the development of therapeutic cloning. It is necessary to improve many techniques and reinforce the research of some basic theories, then somatic nuclear transfer and therapeutic cloning may apply to agriculture reproduction and benefit to human life better.

  6. The First Human Cloned Embryo.

    Science.gov (United States)

    Cibelli, Jose B.; Lanza, Robert P.; West, Michael D.; Ezzell, Carol

    2002-01-01

    Describes a process known as parthenogenesis which produces cloned, early-stage embryos and human embryos generated only from eggs. Speculates that this technology puts therapeutic cloning within reach. (DDR)

  7. Human cloning. Fact or fiction

    International Nuclear Information System (INIS)

    Abushama, Mandy D.; Ahmed, Badreldeen I.

    2003-01-01

    Cloning is the production of one or more individual plants or animals that are genetically identical to other plant, animal or human. Scientists even demonstrated that they were able to clone frog tadpoles from frog embryonic cells using nuclear transfer.Many animals have been cloned from adult cells using nuclear transfer. Somatic cell nuclear transfer which refers to the transfer of the nucleous from a somatic cell to an egg cell. Article further deals with benefits and misuses of human cloning

  8. The influence of alternative plant propagation and stand establishment techniques on survival and growth of eastern cottonwood (Populus deltoids Bartr.) clones

    Science.gov (United States)

    Donald J. Kaczmarek; Randall Rousseau; Jeff A. Wright; Brian Wachelka

    2014-01-01

    Four eastern cottonwood clones, including standard operational clone ST66 and three advanced clonal selections were produced and included in a test utilizing five different plant propagation methods. Despite relatively large first-year growth differences among clones, all clones demonstrated similar responses to the treatments and clone × cutting treatment interactions...

  9. An Innovative Hybrid Loop-Pool SFR Design and Safety Analysis Methods: Today and Tomorrow

    International Nuclear Information System (INIS)

    Hongbin Zhang; Haihua Zhao; Vincent Mousseau

    2008-01-01

    Investment in commercial sodium cooled fast reactor (SFR) power plants will become possible only if SFRs achieve economic competitiveness as compared to light water reactors and other Generation IV reactors. Toward that end, we have launched efforts to improve the economics and safety of SFRs from the thermal design and safety analyses perspectives at Idaho National Laboratory. From the thermal design perspective, an innovative hybrid loop-pool SFR design has been proposed. This design takes advantage of the inherent safety of a pool design and the compactness of a loop design to further improve economics and safety. From the safety analyses perspective, we have initiated an effort to develop a high fidelity reactor system safety code

  10. Innovative test method for the estimation of the foaming tendency of substrates for biogas plants.

    Science.gov (United States)

    Moeller, Lucie; Eismann, Frank; Wißmann, Daniel; Nägele, Hans-Joachim; Zielonka, Simon; Müller, Roland A; Zehnsdorf, Andreas

    2015-07-01

    Excessive foaming in anaerobic digestion occurs at many biogas plants and can cause problems including plugged gas pipes. Unfortunately, the majority of biogas plant operators are unable to identify the causes of foaming in their biogas reactor. The occurrence of foaming is often related to the chemical composition of substrates fed to the reactor. The consistency of the digestate itself is also a crucial part of the foam formation process. Thus, no specific recommendations concerning substrates can be given in order to prevent foam formation in biogas plants. The safest way to avoid foaming is to test the foaming tendency of substrates on-site. A possible solution is offered by an innovative foaming test. With the help of this tool, biogas plant operators can evaluate the foaming disposition of new substrates prior to use in order to adjust the composition of substrate mixes. Copyright © 2015 Elsevier Ltd. All rights reserved.

  11. Innovative methods and tools for professionals working in supported living services for intellectually disabled persons.

    Science.gov (United States)

    Gruiz, Katalin

    2015-01-01

    Autonomy of mid-seriously and seriously intellectually disabled persons is encouraged both by legislations on human rights and the modern social care and services. The process leading to the maximum possible autonomy is illustrated by a developmental spiral in our model. Specialty of the development is that the personal educational projects are realized during everyday activities. The process requires conscious professionals with an empowering and motivating attitude, with adult relationship to the intellectually disabled persons and versatile skills and tools. In this educational relationship the social professional and the supported person are equal partners moving together along the spiral of human development. An innovative tool-battery has been developed aiding support-staff in the 'pedagogical' task embedded into everyday social services. The tool-battery and its first application in supported living services of the Hungarian Down Foundation are introduced in this paper.

  12. Turn of the century refueling: A review of innovations in early gasoline refueling methods and analogies for hydrogen

    International Nuclear Information System (INIS)

    Melaina, Marc W.

    2007-01-01

    During the first decades of the 20th century, a variety of gasoline refueling methods supported early US gasoline vehicles and successfully alleviated consumer concerns over refueling availability. The refueling methods employed included cans, barrels, home refueling outfits, parking garage refueling facilities, mobile stations, hand carts and curb pumps. Only after robust markets for gasoline vehicles had been firmly established did the gasoline service station become the dominant refueling method. The present study reviews this history and draws analogies with current and future efforts to introduce hydrogen as a fuel for vehicles. These comparisons hold no predictive power; however, there is heuristic value in an historical review of the first successful and large-scale introduction of a vehicle fuel. From an energy policy perspective, these comparisons reinforce the importance of a long-term and portfolio approach to support for technology development and innovation

  13. The Methodical Bases of Assessment of Innovation Processes in the Sphere of Power Generation with Use of Opportunities of Cognitive Modeling

    Directory of Open Access Journals (Sweden)

    Vikhlyaeva Nataliya V.

    2017-10-01

    Full Text Available The article presents the methodical bases of assessing the innovation processes of power generating companies with use of opportunities of cognitive modeling. It is specified that the method of cognitive modeling allows to remove contradictions concerning the interrelationship of the factors determining the innovation processes of the energy generating companies that usually arise when the results of research on the elements of the innovation system are being coordinated. Based on the arguments and practical examples, provided in the article, it was concluded that application of the method of cognitive modeling in the process of assessing the innovative capabilities of energy-generating companies is a sufficiently flexible and effective tool for supporting decision-making on strategic directions of their development.

  14. Innovative learning for innovation

    NARCIS (Netherlands)

    Dr.Ir. Hay Geraedts

    2010-01-01

    Introduction Innovation is crucial for companies who have to react to constantly changing markets. Several national and European research institutes stress the importance of developing innovation for small and medium size enterprises (SMEs). This was a trigger to design a minor on strategic

  15. Group Innovation Ability of Agricultural Technological Innovation Strategic Alliance

    Institute of Scientific and Technical Information of China (English)

    Chishun; MA; Jintian; YU

    2013-01-01

    Agricultural technological innovation strategic alliance, as an important form of strategic alliance, has steadily strengthened the collaborative management among organizations and raised competitive power with the backing of improved group innovation ability. This article studies innovation ability from individual innovation ability to the group innovation ability. Firstly, basic connotation of group innovation ability is to be concluded through the comparison of individual and group innovation ability. Secondly, evaluation index system is to be established based on the influencing factors of the group innovation ability of agricultural technological innovation strategic alliance and evaluation is based on three dimensions, namely organization technological innovation ability, alliance collaborative innovation ability as well as innovation environment. Furthermore, basic methods for promoting the group innovation ability of alliance are to be proposed.

  16. Three concepts of cloning in human beings.

    Science.gov (United States)

    Cui, Ke-Hui

    2005-07-01

    Human cloning, organ cloning and tissue cloning are various types of cloning that occur at different levels with different methodologies. According to three standards of terminology for an embryo (fertilization through germ cells, development in the uterus and having the potential to produce a human life), tissue cloning and type I organ cloning will not produce an embryo. In contrast, human cloning and type II organ cloning will produce an embryo. Thus, only non-germinal tissue cloning and type I organ cloning are beyond the ethical question and will not change human beings as a species. Using cloned tissues to make new tissues or organs is promising for the future of medicine.

  17. Optimally cloned binary coherent states

    Science.gov (United States)

    Müller, C. R.; Leuchs, G.; Marquardt, Ch.; Andersen, U. L.

    2017-10-01

    Binary coherent state alphabets can be represented in a two-dimensional Hilbert space. We capitalize this formal connection between the otherwise distinct domains of qubits and continuous variable states to map binary phase-shift keyed coherent states onto the Bloch sphere and to derive their quantum-optimal clones. We analyze the Wigner function and the cumulants of the clones, and we conclude that optimal cloning of binary coherent states requires a nonlinearity above second order. We propose several practical and near-optimal cloning schemes and compare their cloning fidelity to the optimal cloner.

  18. Dose dependency of the frequency of micronucleated binucleated clone cells and of division related median clone sizes difference. Pt. 2

    International Nuclear Information System (INIS)

    Hagemann, G,; Kreczik, A.; Treichel, M.

    1996-01-01

    Following irradiation of the progenitor cells the clone growth of CHO cells decreases as a result of cell losses. Lethally acting expressions of micronuclei are produced by heritable lethal mutations. The dependency of the frequency of micronucleated binucleated clone cells and of the median clone sizes difference on the radiation dose was measured and compared to non-irradiated controls. Using the cytokinesis-block-micronucleus-method binucleated cells with micronuclei were counted as ratio of all binucleated cells within a clone size distribution. This ratio (shortened: micronucleus yield) was determined for all clone size distributions, which had been exposed to different irradiation doses and incubation times. The micronucleus yields were compared to the corresponding median clone sizes differences. The micronucleus yield is linearly dependent on the dose and is independent of the incubation time. The same holds true for the division related median clone sizes difference, which as a result is also linearly dependent on the micronucleus yield. Due to the inevitably errors of the cell count of micronucleated binucleated cells, an automatic measurement of the median clone sizes differences is the preferred method for evaluation of cellular radiation sensitivity for heritable lethal mutations. This value should always be determined in addition, if clone survival fractions are used as predictive test because it allows for an estimation of the remission probability of surviving cells. (orig.) [de

  19. The Diabetes Evaluation Framework for Innovative National Evaluations (DEFINE): Construct and Content Validation Using a Modified Delphi Method.

    Science.gov (United States)

    Paquette-Warren, Jann; Tyler, Marie; Fournie, Meghan; Harris, Stewart B

    2017-06-01

    In order to scale-up successful innovations, more evidence is needed to evaluate programs that attempt to address the rising prevalence of diabetes and the associated burdens on patients and the healthcare system. This study aimed to assess the construct and content validity of the Diabetes Evaluation Framework for Innovative National Evaluations (DEFINE), a tool developed to guide the evaluation, design and implementation with built-in knowledge translation principles. A modified Delphi method, including 3 individual rounds (questionnaire with 7-point agreement/importance Likert scales and/or open-ended questions) and 1 group round (open discussion) were conducted. Twelve experts in diabetes, research, knowledge translation, evaluation and policy from Canada (Ontario, Quebec and British Columbia) and Australia participated. Quantitative consensus criteria were an interquartile range of ≤1. Qualitative data were analyzed thematically and confirmed by participants. An importance scale was used to determine a priority multi-level indicator set. Items rated very or extremely important by 80% or more of the experts were reviewed in the final group round to build the final set. Participants reached consensus on the content and construct validity of DEFINE, including its title, overall goal, 5-step evaluation approach, medical and nonmedical determinants of health schematics, full list of indicators and associated measurement tools, priority multi-level indicator set and next steps in DEFINE's development. Validated by experts, DEFINE has the right theoretic components to evaluate comprehensively diabetes prevention and management programs and to support acquisition of evidence that could influence the knowledge translation of innovations to reduce the burden of diabetes. Copyright © 2017 The Authors. Published by Elsevier Inc. All rights reserved.

  20. [Media, cloning, and bioethics].

    Science.gov (United States)

    Costa, S I; Diniz, D

    2000-01-01

    This article was based on an analysis of three hundred articles from mainstream Brazilian periodicals over a period of eighteen months, beginning with the announcement of the Dolly case in February 1997. There were two main objectives: to outline the moral constants in the press associated with the possibility of cloning human beings and to identify some of the moral assumptions concerning scientific research with non-human animals that were published carelessly by the media. The authors conclude that there was a haphazard spread of fear concerning the cloning of human beings rather than an ethical debate on the issue, and that there is a serious gap between bioethical reflections and the Brazilian media.

  1. Innovative test method for the estimation of the foaming tendency of substrates for biogas plants

    Energy Technology Data Exchange (ETDEWEB)

    Moeller, Lucie, E-mail: lucie.moeller@ufz.de [UFZ – Helmholtz Centre for Environmental Research, Centre for Environmental Biotechnology, Permoserstrasse 15, 04318 Leipzig (Germany); Eismann, Frank, E-mail: info@antoc.de [Eismann & Stöbe GbR, GeoPark, Geb. A12, Bautzner Strasse 67, 04347 Leipzig (Germany); Wißmann, Daniel, E-mail: d.s.wissmann@gmx.de [University of Hohenheim, State Institute of Agricultural Engineering and Bioenergy (LA740), Garbenstrasse 9, 70599 Stuttgart (Germany); Nägele, Hans-Joachim, E-mail: hajo.naegele@uni-hohenheim.de [University of Hohenheim, State Institute of Agricultural Engineering and Bioenergy (LA740), Garbenstrasse 9, 70599 Stuttgart (Germany); Zielonka, Simon, E-mail: simon.zielonka@uni-hohenheim.de [University of Hohenheim, State Institute of Agricultural Engineering and Bioenergy (LA740), Garbenstrasse 9, 70599 Stuttgart (Germany); Müller, Roland A., E-mail: roland.mueller@ufz.de [UFZ – Helmholtz Centre for Environmental Research, Centre for Environmental Biotechnology, Permoserstrasse 15, 04318 Leipzig (Germany); Zehnsdorf, Andreas, E-mail: andreas.zehnsdorf@ufz.de [UFZ – Helmholtz Centre for Environmental Research, Centre for Environmental Biotechnology, Permoserstrasse 15, 04318 Leipzig (Germany)

    2015-07-15

    Graphical abstract: Display Omitted - Highlights: • Foaming in biogas plants depends on the interactions between substrate and digestate. • Foaming tests enable the evaluation of substrate foaming tendency in biogas plants. • Leipzig foam tester enables foaming tests of substrates prior to use. - Abstract: Excessive foaming in anaerobic digestion occurs at many biogas plants and can cause problems including plugged gas pipes. Unfortunately, the majority of biogas plant operators are unable to identify the causes of foaming in their biogas reactor. The occurrence of foaming is often related to the chemical composition of substrates fed to the reactor. The consistency of the digestate itself is also a crucial part of the foam formation process. Thus, no specific recommendations concerning substrates can be given in order to prevent foam formation in biogas plants. The safest way to avoid foaming is to test the foaming tendency of substrates on-site. A possible solution is offered by an innovative foaming test. With the help of this tool, biogas plant operators can evaluate the foaming disposition of new substrates prior to use in order to adjust the composition of substrate mixes.

  2. The Technology of Introduction of Innovation Methods of Management of Development of the Hotel and Restaurant Enterprises on the Basis of Synergistic Approach

    Directory of Open Access Journals (Sweden)

    Davydova Oksana Yu.

    2018-02-01

    Full Text Available The article is aimed at formation of technology of introduction of innovation methods of management of development of hotel and restaurant enterprises on the basis of synergetic approach. The following evolutionary stages of innovative management of enterprises’ development are proposed: preliminary (conjugation; existing (relations and bifurcation; perspective (counterdifferentiation. The technology of introduction of innovation methods of management of development of enterprises of hotel-restaurant industry on the basis of synergistic approach has been developed, confirming existence of synergism in the system of increase of activity potential, opening of new directions of innovative management of development of hotel and restaurant enterprises. The indicators of synergistic effect are determined as follows: high level of performance indicators of enterprise of hotel-restaurant industry; adequate response to changes in the external and internal environment; adaptation to predictable and unpredictable crisis phenomena; improving the quality of products and services; creation and improvement of positive image in both internal and external markets, etc.

  3. Probabilistic cloning of equidistant states

    International Nuclear Information System (INIS)

    Jimenez, O.; Roa, Luis; Delgado, A.

    2010-01-01

    We study the probabilistic cloning of equidistant states. These states are such that the inner product between them is a complex constant or its conjugate. Thereby, it is possible to study their cloning in a simple way. In particular, we are interested in the behavior of the cloning probability as a function of the phase of the overlap among the involved states. We show that for certain families of equidistant states Duan and Guo's cloning machine leads to cloning probabilities lower than the optimal unambiguous discrimination probability of equidistant states. We propose an alternative cloning machine whose cloning probability is higher than or equal to the optimal unambiguous discrimination probability for any family of equidistant states. Both machines achieve the same probability for equidistant states whose inner product is a positive real number.

  4. Bureaucracy and Innovation Revisited

    DEFF Research Database (Denmark)

    Hartmann, Mia Koss; Koss Hartmann, Rasmus

    , but by problematizing the relationship directly. Inspired by prior ethnographic studies of coercive bureaucracies and theories of secrecy and hiding behavior, we propose that coercive bureaucracies do not necessarily impede innovation, but rather create the appearance of doing so. Coercive bureaucracies create......, supplemented by innovation histories, surveys and expert evaluations. This argument implies that future research examines more deeply the organizational processes and practices by which innovation might occur even in coercive bureaucracies. Further, it encourages innovation research methods that rely less...

  5. Biodiversity versus cloning

    International Nuclear Information System (INIS)

    Jaramillo T, Jose Hernan

    1998-01-01

    The announcement has been made on the cloning of mice in these days and he doesn't stop to miss, because the world lives a stage where conscience of the protection is creating that should be given to the biodiversity. It is known that alone we won't subsist and the protection of the means and all that contains that environment is of vital importance for the man. But it is also known that the vegetables and animal transgenic that they come to multiply the species have appeared that we prepare. The transgenic has been altered genetically, for substitution of one or more genes of other species, inclusive human genes. This represents an improvement compared with the investigations that gave origin to the cloning animal. But it is necessary to notice that to it you arrived through the cloning. This year 28 million hectares have been sowed in cultivations of transgenic seeds and there is around 700 bovine transgenic whose milk contains a necessary protein in the treatment of the man's illnesses

  6. Towards an understanding of British public attitudes concerning human cloning.

    Science.gov (United States)

    Shepherd, Richard; Barnett, Julie; Cooper, Helen; Coyle, Adrian; Moran-Ellis, Jo; Senior, Victoria; Walton, Chris

    2007-07-01

    The ability of scientists to apply cloning technology to humans has provoked public discussion and media coverage. The present paper reports on a series of studies examining public attitudes to human cloning in the UK, bringing together a range of quantitative and qualitative methods to address this question. These included a nationally representative survey, an experimental vignette study, focus groups and analyses of media coverage. Overall the research presents a complex picture of attitude to and constructions of human cloning. In all of the analyses, therapeutic cloning was viewed more favourably than reproductive cloning. However, while participants in the focus groups were generally negative about both forms of cloning, and this was also reflected in the media analyses, quantitative results showed more positive responses. In the quantitative research, therapeutic cloning was generally accepted when the benefits of such procedures were clear, and although reproductive cloning was less accepted there was still substantial support. Participants in the focus groups only differentiated between therapeutic and reproductive cloning after the issue of therapeutic cloning was explicitly raised; initially they saw cloning as being reproductive cloning and saw no real benefits. Attitudes were shown to be associated with underlying values associated with scientific progress rather than with age, gender or education, and although there were a few differences in the quantitative data based on religious affiliation, these tended to be small effects. Likewise in the focus groups there was little direct appeal to religion, but the main themes were 'interfering with nature' and the 'status of the embryo', with the latter being used more effectively to try to close down further discussion. In general there was a close correspondence between the media analysis and focus group responses, possibly demonstrating the importance of media as a resource, or that the media reflect

  7. Innovation quality in knowledge cities

    DEFF Research Database (Denmark)

    Makkonen, Teemu; Inkinen, Tommi

    2014-01-01

    Innovation awards have for long attracted policy makers as a method for innovation promotion. Still, academic research on innovation awards has thus far received little attention. In particular, empirical studies on the motives to enter award competitions and the realized impacts of winning an in...... for innovation promotion, alongside innovation inducement policies including tax reductions and direct funding, as they produce significant positive effects for the award winning companies, and an additional indicator of innovation quality in the context of knowledge cities....

  8. National Study of Excellence and Innovation in Physical Therapist Education: Part 1-Design, Method, and Results.

    Science.gov (United States)

    Jensen, Gail M; Nordstrom, Terrence; Mostrom, Elizabeth; Hack, Laurita M; Gwyer, Janet

    2017-09-01

    The Carnegie Foundation for the Advancement of Teaching commissioned the Preparation for the Professions Program, a qualitative study of professional education in 5 professions: medicine, nursing, law, engineering, and clergy. These studies identified curricular structures, instructional practices, assessment approaches, and environmental characteristics that support the preparation of professionals and led to educational reforms. The physical therapy profession has not had any in-depth, national investigation of physical therapist education since the Catherine Worthingham studies conducted more than 50 years ago. This research was a Carnegie-type study, investigating elements of excellence and innovation in academic and clinical physical therapist education in the United States. Five physical therapist education researchers from across the United States used a qualitative multiple-case study design. Six academic and 5 clinical programs were selected for the study. The academic institutions and clinical agencies studied were diverse in size, institutional setting, geography, and role in residency education. Qualitative case studies were generated from review of artifacts, field observations, and interviews (individual and focus group), and they provided the data for the study. A conceptual framework grounded in 3 major dimensions was generated, with 8 supporting elements: (1) culture of excellence (shared beliefs and values, leadership and vision, drive for excellence, and partnerships), (2) praxis of learning (signature pedagogy, practice-based learning, creating adaptive learners, and professional formation), and (3) organizational structures and resources. Building on the work of the Carnegie Foundation's Preparation for the Professions Program, a conceptual model was developed, representing the dimensions and elements of excellence in physical therapist education that is centered on the foundational importance of a nexus of linked and highly valued aims of

  9. An innovative method for extracting isotopic information from low-resolution gamma spectra

    International Nuclear Information System (INIS)

    Miko, D.; Estep, R.J.; Rawool-Sullivan, M.W.

    1998-01-01

    A method is described for the extraction of isotopic information from attenuated gamma ray spectra using the gross-count material basis set (GC-MBS) model. This method solves for the isotopic composition of an unknown mixture of isotopes attenuated through an absorber of unknown material. For binary isotopic combinations the problem is nonlinear in only one variable and is easily solved using standard line optimization techniques. Results are presented for NaI spectrum analyses of various binary combinations of enriched uranium, depleted uranium, low burnup Pu, 137 Cs, and 133 Ba attenuated through a suite of absorbers ranging in Z from polyethylene through lead. The GC-MBS method results are compared to those computed using ordinary response function fitting and with a simple net peak area method. The GC-MBS method was found to be significantly more accurate than the other methods over the range of absorbers and isotopic blends studied

  10. Experimental innovations in surface science a guide to practical laboratory methods and instruments

    CERN Document Server

    Yates, John T

    2015-01-01

    This book is a new edition of a classic text on experimental methods and instruments in surface science. It offers practical insight useful to chemists, physicists, and materials scientists working in experimental surface science. This enlarged second edition contains almost 300 descriptions of experimental methods. The more than 50 active areas with individual scientific and measurement concepts and activities relevant to each area are presented in this book. The key areas covered are: Vacuum System Technology, Mechanical Fabrication Techniques, Measurement Methods, Thermal Control, Delivery of Adsorbates to Surfaces, UHV Windows, Surface Preparation Methods, High Area Solids, Safety. The book is written for researchers and graduate students.

  11. Knowledge and Cooperation Determinants of Innovation Networks: A Mixed-Methods Approach to the Case of Portugal

    Directory of Open Access Journals (Sweden)

    Hugo Pinto

    2015-04-01

    Full Text Available Systemic perspectives of innovation integrate complex interrelations among enterprise, science and technology, and governance spheres. Innovation networks are crucial within innovation systems and refer to the linkages of a variety of actors with the purpose of innovation. In this article, the determinants of innovation networks are analyzed using a qualitative original database of online information about 623 organizations in Portugal. A binary econometric regression for all types of entities is estimated. The model underlines that actors using external technologies and promoting knowledge are more likely to innovate. In parallel, actors that are involved in managing and supporting entrepreneurship have a smaller probability to do it. Advanced firms and universities are the actors more willing to dynamically innovate. Specific models for firms and universities create a direct comparison between the determinants in both collectives. While promoting knowledge and specific orientation towards innovation is essential for firms it is not relevant for universities. Managing knowledge is the crucial catalyst for the innovation practices in universities. External technological linkages are essential for both types of actors in the creation of innovation networks. The article concludes with policy implications regarding the support of cooperation activities to instigate innovation.

  12. An innovative method for ideal and resistive MHD stability analysis of tokamaks

    International Nuclear Information System (INIS)

    Tokuda, S.

    2001-01-01

    An advanced asymptotic matching method of ideal and resistive MHD stability analysis in tokamaks is reported. A solution method for the two dimensional Newcomb equation, a dispersion relation for an unstable ideal MHD mode in tokamaks and a new scheme for solving resistive MHD inner layer equations as an initial value problem are reported. (author)

  13. An innovative method for ideal and resistive MHD stability analysis of tokamaks

    International Nuclear Information System (INIS)

    Tokuda, S.

    2001-01-01

    An advanced asymptotic matching method of ideal and resistive MHD stability analysis in tokamak is reported. The report explains a solution method of two-dimensional Newcomb equation, dispersion relation for an unstable ideal MHD mode in tokamak, and a new scheme for solving resistive MHD inner layer equations as an initial-value problem. (author)

  14. EVALUATION OF THE CARBON FOOTPRINT OF AN INNOVATIVE SEWER REHABILITATION METHOD - abstract

    Science.gov (United States)

    A benefit of trenchless methods touted by many practitioners when compared to open cut construction is lower carbon dioxide emissions. In an attempt to verify these claims, tools have been developed that calculate the environmental impact of traditional open cut methods and commo...

  15. EVALUATION OF THE CARBON FOOTPRINT OF AN INNOVATIVE SEWER REHABILITATION METHOD

    Science.gov (United States)

    A benefit of trenchless methods touted by many practitioners when compared to open cut construction is lower carbon dioxide emissions. In an attempt to verify these claims, tools have been developed that calculate the environmental impact of traditional open cut methods and commo...

  16. Applicability of an innovative steroid-profiling method to determine synthetic growth promoter abuse in cattle

    NARCIS (Netherlands)

    Blokland, M.H.; Tricht, van E.F.; Ginkel, van L.A.; Sterk, S.S.

    2017-01-01

    A robust LC–MS/MS method was developed to quantify a large number of phase I and phase II steroids in urine. The decision limit is for most compounds lower than 1 ng ml−1 with a measurement uncertainty smaller than 30%. The method is fully validated and was applied to assess the influence of

  17. Facilitating User Driven Innovation – A Study of Methods and Tools at Herlev Hospital

    DEFF Research Database (Denmark)

    Fronczek-Munter, Aneta

    2011-01-01

    are actively involved as co-creators. The paper describes the process and its phases, as well as reflects on the results of the user involvement and specific methods. Depending on the methods used at the workshops the participants/users had different focus, changed the priorities and developed different...

  18. Facilitating User Driven Innovation – A Study of Methods and Tools at Herlev Hospital

    DEFF Research Database (Denmark)

    Fronczek-Munter, Aneta

    2012-01-01

    are actively involved as co-creators. The paper describes the process and its phases, as well as reflects on the results of the user involvement and specific methods. Depending on the methods used at the workshops the participants/users had different focus, changed the priorities and developed different...

  19. Developing Employability Skills in Information System Graduates: Traditional vs. Innovative Teaching Methods

    Science.gov (United States)

    Osmani, Mohamad; Hindi, Nitham M.; Weerakkody, Vishanth

    2018-01-01

    It is widely acknowledged that traditional teaching methods such as lectures, textbooks and case study techniques on their own are not adequate to improving the most in-demand employability skills for graduates. The aim of this article is to explore the potential impact that novel learning and teaching methods can have on improving the…

  20. EFFECT OF CORPORATE INNOVATION ABILITY ON THE CHOICE BETWEEN PRODUCT INNOVATION AND PROCESS INNOVATION

    OpenAIRE

    Zeng Wu; Tian Zhilong

    2010-01-01

    With the method of duopoly game theory, the effect of firms’ innovation ability on the choice between product and process innovation is studied. The concept of cost coefficient of product innovation is introduced, and the criterion equation for the innovation type is derived. The following conclusions are made: the more the product innovation ability, the more the possibility for the firms to carry out the product innovation in both the Bertrand and the Cournot competitions. Wi...

  1. Ethical issues in animal cloning.

    Science.gov (United States)

    Fiester, Autumn

    2005-01-01

    The issue of human reproductive cloning has recently received a great deal attention in public discourse. Bioethicists, policy makers, and the media have been quick to identify the key ethical issues involved in human reproductive cloning and to argue, almost unanimously, for an international ban on such attempts. Meanwhile, scientists have proceeded with extensive research agendas in the cloning of animals. Despite this research, there has been little public discussion of the ethical issues raised by animal cloning projects. Polling data show that the public is decidedly against the cloning of animals. To understand the public's reaction and fill the void of reasoned debate about the issue, we need to review the possible objections to animal cloning and assess the merits of the anti-animal cloning stance. Some objections to animal cloning (e.g., the impact of cloning on the population of unwanted animals) can be easily addressed, while others (e.g., the health of cloned animals) require more serious attention by the public and policy makers.

  2. Logistic innovations in transport

    Directory of Open Access Journals (Sweden)

    Mirosław Antonowicz

    2014-03-01

    Full Text Available Introduction: The article discusses the issue of logistic innovations in transport. The essentials of logistic innovations in transport together with some examples of specific innovations are presented. The role of the client's needs in transport innovations is indicated. The most vital postulates affecting the innovativeness of shipping companies and derived from the author's experience as well as scholarly publications, are time, safety, reliability as well as comprehensiveness of service offer. Following the analysis of the issue, and on the grounds of Kaizen's and Lean's method, the concept of continuous innovations is suggested as very useful for the development of transport. The potential of clusters as the source of logistic innovations in transport is emphasised. Methods: The discussion of the issue was preceded by the author's analysis of written sources on innovativeness, the evaluation of ratings of innovativeness as well as the analysis of rewarded innovative solutions in transport subsequent to the businesses participation in the programme of innovative solutions in transport. The role of innovation practical business operations is argued following the analysis of some strategic documents such as: 2011 White Paper and the Strategy for the Development of Transport by 2020 adopted by the Polish government in 2013. Aim: The aim of the article is to present the role and significance of the issue of logistic innovations in transport and to cite instances of practical solutions implemented by shipping companies, the solutions which resulted in measurable effects. Following the author's observation of the instances of innovative solutions as well as his analysis of the ratings of innovativeness, the article aims to present the conclusions as for the specific kinds of activities which are indispensable to foster innovativeness in transport. Conclusions: The conclusions derived from the author's analyses and observations show that logistic

  3. Innovative methods for calculation of freeway travel time using limited data : final report.

    Science.gov (United States)

    2008-01-01

    Description: Travel time estimations created by processing of simulated freeway loop detector data using proposed method have been compared with travel times reported from VISSIM model. An improved methodology was proposed to estimate freeway corrido...

  4. Innovative Columnar Type of Grid Array SJ BIST HALT Method, Phase I

    Data.gov (United States)

    National Aeronautics and Space Administration — Ridgetop will develop a superior method for testing and qualifying columnar type of grid arrays such as field programmable gate arrays (FPGAs) packaged in column...

  5. EVALUATION OF THE CARBON FOOTPRINT OF INNOVATIVE WATER MAIN REHABILITATION TECHNOLOGIES VS. OPEN CUT METHODS

    Science.gov (United States)

    A major benefit of trenchless rehabilitation technologies touted by many practitioners when comparing their products with tradition open cut construction methods is lower carbon dioxide (CO2) emissions. In an attempt to verify these claims, multiple tools have been dev...

  6. Innovative methods for calculation of freeway travel time using limited data : executive summary report.

    Science.gov (United States)

    2008-08-01

    ODOTs policy for Dynamic Message Sign : utilization requires travel time(s) to be displayed as : a default message. The current method of : calculating travel time involves a workstation : operator estimating the travel time based upon : observati...

  7. Strengthening regional innovation through network-based innovation brokering

    OpenAIRE

    Svare, Helge; Gausdal, Anne Haugen

    2015-01-01

    The primary objective of this paper is to demonstrate how regional innovation system theory may be translated into manageable micro-level methods with the potential for strengthening the productive dynamics of a regional innovation system. The paper meets this objective by presenting network-based innovation brokering (NBIB), a practical method designed using insights from regional innovation system theory and trust theory. Five cases from two Norwegian regional innovation networks show that ...

  8. Adding to The Innovation Taxonomy

    Directory of Open Access Journals (Sweden)

    Bengt-Arne Vedin

    2014-02-01

    Full Text Available Innovation might be described in a number of categories: radical, disruptive, incremental, or, by type, product, process, and service innovation. Social and conceptual innovations are decidedly more abstract. In recent years, open and also frugal innovation have been defined. This essay suggests and contemplates four additional concepts. Mesovation is in between radical and incremental innovation. Exovation stands for systematic ways to reduce barriers to innovation. Metovations are novel methods or systems affecting conditions for innovation. Lastly, ynnovation would be cultural innovation.

  9. Citric acid facilitated thermal treatment: An innovative method for the remediation of mercury contaminated soil

    International Nuclear Information System (INIS)

    Ma, Fujun; Peng, Changsheng; Hou, Deyi; Wu, Bin; Zhang, Qian; Li, Fasheng; Gu, Qingbao

    2015-01-01

    Highlights: • Hg content was reduced to <1.5 mg/kg when treated at 400 °C with citric acid. • The treated soil retained most of its original soil physicochemical properties. • Proton provided by citric acid facilitates thermal removal of mercury. • This thermal treatment method is expected to reduce energy input by 35%. - Abstract: Thermal treatment is a promising technology for the remediation of mercury contaminated soils, but it often requires high energy input at heating temperatures above 600 °C, and the treated soil is not suitable for agricultural reuse. The present study developed a novel method for the thermal treatment of mercury contaminated soils with the facilitation of citric acid (CA). A CA/Hg molar ratio of 15 was adopted as the optimum dosage. The mercury concentration in soils was successfully reduced from 134 mg/kg to 1.1 mg/kg when treated at 400 °C for 60 min and the treated soil retained most of its original soil physiochemical properties. During the treatment process, CA was found to provide an acidic environment which enhanced the volatilization of mercury. This method is expected to reduce energy input by 35% comparing to the traditional thermal treatment method, and lead to agricultural soil reuse, thus providing a greener and more sustainable remediation method for treating mercury contaminated soil in future engineering applications.

  10. Citric acid facilitated thermal treatment: An innovative method for the remediation of mercury contaminated soil

    Energy Technology Data Exchange (ETDEWEB)

    Ma, Fujun [State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012 (China); Peng, Changsheng [The Key Lab of Marine Environmental Science and Ecology, Ministry of Education, Ocean University of China, Qingdao 266100 (China); Hou, Deyi [Geotechnical and Environmental Research Group, Department of Engineering, University of Cambridge, Cambridge CB2 1PZ (United Kingdom); Wu, Bin; Zhang, Qian; Li, Fasheng [State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012 (China); Gu, Qingbao, E-mail: guqb@craes.org.cn [State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012 (China)

    2015-12-30

    Highlights: • Hg content was reduced to <1.5 mg/kg when treated at 400 °C with citric acid. • The treated soil retained most of its original soil physicochemical properties. • Proton provided by citric acid facilitates thermal removal of mercury. • This thermal treatment method is expected to reduce energy input by 35%. - Abstract: Thermal treatment is a promising technology for the remediation of mercury contaminated soils, but it often requires high energy input at heating temperatures above 600 °C, and the treated soil is not suitable for agricultural reuse. The present study developed a novel method for the thermal treatment of mercury contaminated soils with the facilitation of citric acid (CA). A CA/Hg molar ratio of 15 was adopted as the optimum dosage. The mercury concentration in soils was successfully reduced from 134 mg/kg to 1.1 mg/kg when treated at 400 °C for 60 min and the treated soil retained most of its original soil physiochemical properties. During the treatment process, CA was found to provide an acidic environment which enhanced the volatilization of mercury. This method is expected to reduce energy input by 35% comparing to the traditional thermal treatment method, and lead to agricultural soil reuse, thus providing a greener and more sustainable remediation method for treating mercury contaminated soil in future engineering applications.

  11. An innovative method for coordinate measuring machine one-dimensional self-calibration with simplified experimental process.

    Science.gov (United States)

    Fang, Cheng; Butler, David Lee

    2013-05-01

    In this paper, an innovative method for CMM (Coordinate Measuring Machine) self-calibration is proposed. In contrast to conventional CMM calibration that relies heavily on a high precision reference standard such as a laser interferometer, the proposed calibration method is based on a low-cost artefact which is fabricated with commercially available precision ball bearings. By optimizing the mathematical model and rearranging the data sampling positions, the experimental process and data analysis can be simplified. In mathematical expression, the samples can be minimized by eliminating the redundant equations among those configured by the experimental data array. The section lengths of the artefact are measured at arranged positions, with which an equation set can be configured to determine the measurement errors at the corresponding positions. With the proposed method, the equation set is short of one equation, which can be supplemented by either measuring the total length of the artefact with a higher-precision CMM or calibrating the single point error at the extreme position with a laser interferometer. In this paper, the latter is selected. With spline interpolation, the error compensation curve can be determined. To verify the proposed method, a simple calibration system was set up on a commercial CMM. Experimental results showed that with the error compensation curve uncertainty of the measurement can be reduced to 50%.

  12. Local cloning of CAT states

    International Nuclear Information System (INIS)

    Rahaman, Ramij

    2011-01-01

    In this Letter we analyze the (im)possibility of the exact cloning of orthogonal three-qubit CAT states under local operation and classical communication (LOCC) with the help of a restricted entangled state. We also classify the three-qubit CAT states that can (not) be cloned under LOCC restrictions and extend the results to the n-qubit case. -- Highlights: → We analyze the (im)possibility of exact cloning of orthogonal CAT states under LOCC. → We also classify the set of CAT states that can(not) be cloned by LOCC. → No set of orthogonal CAT states can be cloned by LOCC with help of similar CAT state. → Any two orthogonal n-qubit GHZ-states can be cloned by LOCC with help of a GHZ state.

  13. Lessons learned from cloning dogs.

    Science.gov (United States)

    Kim, M J; Oh, H J; Kim, G A; Park, J E; Park, E J; Jang, G; Ra, J C; Kang, S K; Lee, B C

    2012-08-01

    The aim of this article is to review dog cloning research and to suggest its applications based on a discussion about the normality of cloned dogs. Somatic cell nuclear transfer was successfully used for production of viable cloned puppies despite limited understanding of in vitro dog embryo production. Cloned dogs have similar growth characteristics to those born from natural fertilization, with no evidence of serious adverse effects. The offspring of cloned dogs also have similar growth performance and health to those of naturally bred puppies. Therefore, cloning in domestic dogs can be applied as an assisted reproductive technique to conserve endangered species, to treat sterile canids or aged dogs, to improve reproductive performance of valuable individuals and to generate disease model animals. © 2012 Blackwell Verlag GmbH.

  14. An innovative treatment method for an aqueous waste from the enhanced oil recovery process

    International Nuclear Information System (INIS)

    Alimahmoodi, M.; Mulligan, C.N.

    2009-01-01

    Anaerobic treatment was evaluated to determine its effectiveness in treating a waste stream from the process of Enhanced Oil Recovery (EOR) to remove solubilized CO 2 (98%) and petroleum hydrocarbons (83%) using formate (2 g/L) and sucrose (2.5 g/L) as electron donors in two consecutive reactors. The method of evolutionary operation (EVOP) factorial design was applied to optimize the system and the net energy ratio (NER) of 3.7 was calculated for the system which showed a sustainable biogas production. This method is less complex than other competitive methods, and in addition to its low energy requirements, it can produce CH 4 from CO 2 as a clean source of energy. (author)

  15. Innovation af innovation

    DEFF Research Database (Denmark)

    Harste, Gorm

    2009-01-01

    , at innovation af innovationen forsøges gennemført på en måde, hvor tiden kræves at forholde sig til sin egen tidslighed i form af fremtid, nutid, fortid og ikke mindst i form af samtidighed. I tiden skal vi iagttage, hvordan vi iagttager tiden. Vi dobbelt-koder tiden på samme måde, som forskning forsker i...... organisationssystemerne. De to typer systemer kan noget helt bestemt med fænomenet tid. De kan synkronisere. Analyseres organisationssystemer ser vi, imidlertid at innovation kræver ro. Stærkt innovative systemer er militærsystemet og kunstsystemet, der også inddrages, og hvor vi ser paradokset mellem innovation og...... involution. Tid er med et medium og ikke et lufttomt rum. Tid er end ikke en gasart, men udgør et solidt fluidum, som samfundet bader i og flyder i, konstant i bevægelse. Reformer forudsætter former, og innovation forudsætter involution. Kun sådan muliggøres evolution....

  16. An innovative exercise method to simulate orbital EVA work - Applications to PLSS automatic controls

    Science.gov (United States)

    Lantz, Renee; Vykukal, H.; Webbon, Bruce

    1987-01-01

    An exercise method has been proposed which may satisfy the current need for a laboratory simulation representative of muscular, cardiovascular, respiratory, and thermoregulatory responses to work during orbital extravehicular activity (EVA). The simulation incorporates arm crank ergometry with a unique body support mechanism that allows all body position stabilization forces to be reacted at the feet. By instituting this exercise method in laboratory experimentation, an advanced portable life support system (PLSS) thermoregulatory control system can be designed to more accurately reflect the specific work requirements of orbital EVA.

  17. UVA Methodinnovative approach in economic and strategic management of an enterprise

    OpenAIRE

    Simona Dragomirescu; Daniela Solomon

    2009-01-01

    UVA method has developed starting from the principle “measuring the entire activity of an enterprise with a single unit measure”, announced by Georges Perrin at the beginning of the ‘50’s. The followed aim is that the method would be simple and, respectively, would avoid the global costs treating. As consequence, a minute analysis is needed for every unit of the process. Also, a fundamental principle is imposed: the costs of these activities will generally remain established in relative value...

  18. UVA Methodinnovative approach in economic and strategic management of an enterprise

    Directory of Open Access Journals (Sweden)

    Simona Dragomirescu

    2009-12-01

    Full Text Available UVA method has developed starting from the principle “measuring the entire activity of an enterprise with a single unit measure”, announced by Georges Perrin at the beginning of the ‘50’s. The followed aim is that the method would be simple and, respectively, would avoid the global costs treating. As consequence, a minute analysis is needed for every unit of the process. Also, a fundamental principle is imposed: the costs of these activities will generally remain established in relative values at least for the period when major changes in technology do not occur.

  19. Marketing Innovations in Croatia

    Directory of Open Access Journals (Sweden)

    Ljiljana Božić

    2011-06-01

    Full Text Available The purpose of this paper is to analyze the introduction of marketing innovations by companies in Croatia. This study provides an insight into the innovativeness with regard to the introduction of marketing innovations. It explores the presence of marketing innovations in general and the introduction of four types of marketing innovation (significant changes to the aesthetic design or packaging, new media or techniques for product promotion, new methods for product placement or sales channels and new methods of pricing goods or services. The changes in all four elements of the marketing mix are described. The paper also explores the extent to which the introduction of marketing innovation is related to the companies that introduce other types of innovations.

  20. Cloned cattle derived from a novel zona-free embryo reconstruction system

    NARCIS (Netherlands)

    Oback, B; Wiersema, AT; Gaynor, P; Laible, G; Tucker, FC; Oliver, JE; Miller, AL; Troskie, HE; Wilson, KL; Forsyth, JT; Berg, MC; Cockrem, K; Mcmillan, [No Value; Tervit, HR; Wells, DN

    2003-01-01

    As the demand for cloned embryos and offspring increases, the need arises for the development of nuclear transfer procedures that are improved in both efficiency and ease of operation. Here, we describe a novel zona-free cloning method that doubles the throughput in cloned bovine embryo production

  1. Innovative Solutions for Words with Emphasis: Alternative Methods of Braille Transcription

    Science.gov (United States)

    Kamei-Hannan, Cheryl

    2009-01-01

    The author of this study proposed two alternative methods for transcribing words with emphasis into braille and compared the use of the symbols for emphasis with the current braille code. The results showed that students were faster at locating words presented in one of the alternate formats, but that there was no difference in students' accuracy…

  2. Discrete event simulation of crop operations in sweet pepper in support of work method innovation

    NARCIS (Netherlands)

    Ooster, van 't Bert; Aantjes, Wiger; Melamed, Z.

    2017-01-01

    Greenhouse Work Simulation, GWorkS, is a model that simulates crop operations in greenhouses for the purpose of analysing work methods. GWorkS is a discrete event model that approaches reality as a discrete stochastic dynamic system. GWorkS was developed and validated using cut-rose as a case

  3. Improving patient care trajectories: an innovative quasi-experimental research method for health services.

    Science.gov (United States)

    Campos, Eneida Rached; Moreira-Filho, Djalma de Carvalho; Silva, Marcos Tadeu Nolasco da

    2018-05-01

    Scores to predict treatment outcomes have earned a well-deserved place in healthcare practice. However, when used to help achieve excellence in the care of a given disease, scores should also take into account organizational and social aspects. This article aims to create scores to obtain key variables and its application in the management of care of a given disease. We present a method called Epidemiological Planning for Patient Care Trajectory (PELC) and its application in a research of HIV pediatric patients. This case study is presented by means of two studies. The first study deals with the development of the method PELC. The second is HIV Pediatric case-control study based on PELC method. HIV pediatric research - the first practical PELC application - found these four key variables to the individual quality level care trajectories: adherence to ART, attending at least one appointment with the otolaryngologist, attending at least one appointment with social services, and having missed one or more routine appointments. We believe PELC method can be used in researches about any kind of care trajectories, contributing to quality level advancements in health services, with emphasis on patient safety and equity in healthcare.

  4. Innovative Mixed-Methods Research: Moving beyond Design Technicalities to Epistemological and Methodological Realizations

    Science.gov (United States)

    Riazi, A. Mehdi

    2016-01-01

    Mixed-methods research (MMR), as an inter-discourse (quantitative and qualitative) methodology, can provide applied linguistics researchers the opportunity to draw on and integrate the strengths of the two research methodological approaches in favour of making more rigorous inferences about research problems. In this article, the argument is made…

  5. Orchestrating innovation

    NARCIS (Netherlands)

    Berkers, F.T.H.M.; Klein Woolthuis, R.J.A.; Boer, J. de

    2015-01-01

    Orchestrating Innovation increases the probability of success, minimizing the probability of failure of technological innovations by creating sustained societal and economic value. Orchestrating innovation propagates to take into account and actively involve all relevant stakeholders of the (future)

  6. Unilateral pedicle screws asymmetric tethering: an innovative method to create idiopathic deformity

    Directory of Open Access Journals (Sweden)

    Zhang Xuesong

    2007-10-01

    Full Text Available Abstract Objective To evaluate the feasibility of the method that unilateral pedicle screws asymmetric tethering in concave side in combination with convex rib resection for creating idiopathic deformity. Summary of background data Various methods are performed to create idiopathic deformity. Among these methods, posterior asmmetric tethering of the spine shows satisfying result, but some drawbacks related to the current posterior asymmetric tether were still evident. Materials and methods Unilateral pedicle screws asymmetric tethering was performed to 14 female goats (age: 5–8 week-old, weight: 6–8 kg in concave side in combination with convex rib resection. Dorsoventral and lateral plain radiographs were taken of each thoracic spine in the frontal and sagittal planes right after the surgery and later every 4 weeks. Results All animals ambulated freely after surgery. For technical reasons, 2 goats were excluded (one animal died for anesthetic during the surgery, and one animal was lost for instrumental fail due to postoperative infection. Radiography showed that 11 goats exhibited scoliosis with convex toward to the right side, and as the curve increased with time, only 1 goat showed nonprogressive. The initial scoliosis generated in the progressors after the procedures measured 29.0° on average (range 23.0°–38.5° and increased to 43.0° on average (range 36.0°–58.0° over 8 to 10 weeks. The average progression of 14.0° was measured. The curvature immediately after tethering surgery (the initial Cobb angle did have a highly significant correlation with the final curvature (p Conclusion Unilateral pedicle screws asymmetric tethering is a practical method to create experimental scoliosis, especially for those who would like to study the correction of this deformity.

  7. Innovative methods for maintenance and monitoring of transport preservation of turbo generators; Innovative Methoden zur Aufrechterhaltung und Ueberwachung der Transportkonservierung bei Turbogeneratoren

    Energy Technology Data Exchange (ETDEWEB)

    Rodriguez, Sascha; Pfohl, Rainer [Alstom Power Systems GmbH, Mannheim (Germany); Safari, Hossein [Alstom Switzerland Ltd., Birr (Switzerland); Dubois, Roland; Wittner, Stephan [RWE Technology GmbH, Essen (Germany)

    2012-07-01

    Today, power plant components are manufactured nearly all over the world. Size and weight of many components and also the complexity and length of the transport routes require highly sophisticated logistics based on various means of transport by sea and by land. Critical points during transport include continuous maintenance, monitoring and documentation of the preservation measures set in the factory to withstand the exposure of the components to different and partially adverse ambient conditions. An overview of innovative systems for maintaining and monitoring for example of a 1,300 MVA generator stator. (orig.)

  8. Therapeutic cloning: The ethical limits

    International Nuclear Information System (INIS)

    Whittaker, Peter A.

    2005-01-01

    A brief outline of stem cells, stem cell therapy and therapeutic cloning is given. The position of therapeutic cloning with regard to other embryonic manipulations - IVF-based reproduction, embryonic stem formation from IVF embryos and reproductive cloning - is indicated. The main ethically challenging stages in therapeutic cloning are considered to be the nuclear transfer process including the source of eggs for this and the destruction of an embryo to provide stem cells for therapeutic use. The extremely polarised nature of the debate regarding the status of an early human embryo is noted, and some potential alternative strategies for preparing immunocompatible pluripotent stem cells are indicated

  9. Human cloning and child welfare.

    Science.gov (United States)

    Burley, J; Harris, J

    1999-01-01

    In this paper we discuss an objection to human cloning which appeals to the welfare of the child. This objection varies according to the sort of harm it is expected the clone will suffer. The three formulations of it that we will consider are: 1. Clones will be harmed by the fearful or prejudicial attitudes people may have about or towards them (H1); 2. Clones will be harmed by the demands and expectations of parents or genotype donors (H2); 3. Clones will be harmed by their own awareness of their origins, for example the knowledge that the genetic donor is a stranger (H3). We will show why these three versions of the child welfare objection do not necessarily supply compelling reasons to ban human reproductive cloning. The claim that we will develop and defend in the course of our discussion is that even if it is the case that a cloned child will suffer harms of the type H1-H3, it is none the less permissible to conceive by cloning so long as these cloning-induced welfare deficits are not such as to blight the existence of the resultant child, whoever this may be. PMID:10226914

  10. Molecular profiling of microbial communities from contaminated sources: Use of subtractive cloning methods and rDNA spacer sequences. 1998 annual progress report

    International Nuclear Information System (INIS)

    Robb, F.T.

    1998-01-01

    'The major objective of the research is to provide appropriate sequences and to assemble a high-density DNA array of oligonucleotides that can be used for rapid profiling of microbial populations from polluted areas. The sequences to be assigned to the DNA array are chosen from from cloned genomic DNA sequences (the ribosomal operon, described below) from groundwater at DOE sites containing organic solvents. The sites, Hanford Nuclear Plant and Lawrence Livermore Site 300, have well characterized pollutant histories, which have been provided by the collaborators. At this mid-point of the project, over 60 unique sequence classes of intergenic spacer region have been identified from the first sample site. The use of these sequences as hybridization probes, and their frequency of occurrence, allow a clear distinction between bacterial communities before and after remediation by acetate/nitrate pumping. The authors have developed the hybridization conditions for identifying PCR products in a 96 well format, a versatile alignment and visualization program (acronym: MALIGN) developed by Dr. Dennis Maeder, has been used to align the ISRs, which are variable in length and sometimes in position of the tRNAs. Finally, in collaboration with Dr. W. Chen and Dr. J. Zhou at ORNL, they have significant evidence that mass spectrometer analysis can be used to determine the lengths of PCR amplified intergenic spacer DNA.'

  11. [Cloning and sequence analysis of the DHBV genome of the brown ducks in Guilin region and establishment of the quantitative method for detecting DHBV].

    Science.gov (United States)

    Su, He-Ling; Huang, Ri-Dong; He, Song-Qing; Xu, Qing; Zhu, Hua; Mo, Zhi-Jing; Liu, Qing-Bo; Liu, Yong-Ming

    2013-03-01

    Brown ducks carrying DHBV were widely used as hepatitis B animal model in the research of the activity and toxicity of anti-HBV dugs. Studies showed that the ratio of DHBV carriers in the brown ducks in Guilin region was relatively high. Nevertheless, the characters of the DHBV genome of Guilin brown duck remain unknown. Here we report the cloning of the genome of Guilin brown duck DHBV and the sequence analysis of the genome. The full length of the DHBV genome of Guilin brown duck was 3 027bp. Analysis using ORF finder found that there was an ORF for an unknown peptide other than S-ORF, PORF and C-ORF in the genome of the DHBV. Vector NTI 8. 0 analysis revealed that the unknown peptide contained a motif which binded to HLA * 0201. Aligning with the DHBV sequences from different countries and regions indicated that there were no obvious differences of regional distribution among the sequences. A fluorescence quantitative PCR for detecting DHBV was establishment based on the recombinant plasmid pGEM-DHBV-S constructed. This study laid the groundwork for using Guilin brown duck as a hepatitis B animal model.

  12. Investigation of innovative radiation imaging method and system for radiological environments

    Energy Technology Data Exchange (ETDEWEB)

    Nguyen, H. [School of Biomedical Engineering, Korea University, Seoul (Korea, Republic of); Joung, J., E-mail: jinhun.joung@nucaremed.com [School of Biomedical Engineering, Korea University, Seoul (Korea, Republic of); Nucare, Inc., Incheon (Korea, Republic of); Kim, Y. [School of Biomedical Engineering, Korea University, Seoul (Korea, Republic of); Nucare, Inc., Incheon (Korea, Republic of); Lee, K. [School of Biomedical Engineering, Korea University, Seoul (Korea, Republic of)

    2017-03-01

    We have developed a novel imaging method that can be applied to most applications in the field of radiological environment imaging. It resolves either two-dimensional (2D) or three-dimensional (3D) distributions of radioactive sources in applications for homeland security, environmental monitoring, radiation contamination monitoring, baggage inspection, nuclear power plant monitoring, and more. The proposed imaging method uses a simple detector configured as a radiation-counting detector with spectroscopic capabilities. The detector module consists of two components: a flat field-of-view (FOV) collimator with a 30° FOV opening and a typical single-channel radiation detector made of a 2 in.×2 in. NaI(Tl) scintillator coupled to a 2 in photomultiplier tube (PMT). This simple detector module makes it possible to develop a cost-effective imaging system and provide design freedom in extending the system configuration to include one-dimensional (1D) or 2D detector-array shapes to meet the needs of various applications. One of most distinctive features of the new imaging method is that it uses only a pair of 2D projections to obtain a 3D reconstruction. The projections are measured by the proposed detector module at two positions orthogonal to one another; the measured projections are manipulated to enhance the resolution of the reconstructed 3D image. The imaging method comprises several steps performed consecutively: projection measurement, energy re-binning, projection separation, resolution and attenuation recovery, image reconstruction, and image consolidation and quantitative analysis. The resolution and attenuation recovery step provides the most distinctive and important processing by which the poor quality of projection data is enhanced. Such poor quality is mainly due to the use of a simple detector with a wide-opening flat FOV collimator. Simulation and experimental studies have been conducted to validate the proposed method. In this investigation, we

  13. Innovative classification of methods of the Future-oriented Technology Analysis

    OpenAIRE

    HALICKA, Katarzyna

    2016-01-01

    In the era characterized by significant dynamics of the environment traditional methods of anticipating the future, assuming the immutability of the factors affecting the forecasted phenomenon, may be in the long term ineffective. The modern approach of predicting the future of technology, taking into account the multidimensionality of the environment, is, among other things, the Future-Oriented Technology Analysis (FTA). Designing the FTA research procedure is a complex process, both in orga...

  14. Crack luminescence as an innovative method for detection of fatigue damage

    Directory of Open Access Journals (Sweden)

    R. Makris

    2018-04-01

    Full Text Available Conventional non-destructive testing methods for crack detection provide just a snapshot of fatigue crack evolution at a specific location in the moment of examination. The crack luminescence coating realizes a clear visibility of the entire crack formation. The coating consists of two layers with different properties and functions. The bottom layer emits light as fluorescence under UV radiation. The top layer covers the fluorescing one and prevents the emitting of light in case of no damage at the surface. Several different experiments show that due to the sensitive coating even the early stage of crack formation can be detected. That makes crack luminescence helpful for investigating the incipient crack opening behavior. Cracks can be detected and observed during operation of a structure, making it also very interesting for continuous monitoring. Crack luminescence is a passive method and no skilled professionals are necessary to detect cracks, as for conventional methods. The luminescent light is clearly noticeable by unaided eye observations and also by standard camera equipment, which makes automated crack detection possible as well. It is expected that crack luminescence can reduce costs and time for preventive maintenance and inspection.

  15. A model for scale up of family health innovations in low-income and middle-income settings: a mixed methods study.

    Science.gov (United States)

    Bradley, Elizabeth H; Curry, Leslie A; Taylor, Lauren A; Pallas, Sarah Wood; Talbert-Slagle, Kristina; Yuan, Christina; Fox, Ashley; Minhas, Dilpreet; Ciccone, Dana Karen; Berg, David; Pérez-Escamilla, Rafael

    2012-01-01

    Many family health innovations that have been shown to be both efficacious and cost-effective fail to scale up for widespread use particularly in low-income and middle-income countries (LMIC). Although individual cases of successful scale-up, in which widespread take up occurs, have been described, we lack an integrated and practical model of scale-up that may be applicable to a wide range of public health innovations in LMIC. To develop an integrated and practical model of scale-up that synthesises experiences of family health programmes in LMICs. We conducted a mixed methods study that included in-depth interviews with 33 key informants and a systematic review of peer-reviewed and grey literature from 11 electronic databases and 20 global health agency web sites. We included key informants and studies that reported on the scale up of several family health innovations including Depo-Provera as an example of a product innovation, exclusive breastfeeding as an example of a health behaviour innovation, community health workers (CHWs) as an example of an organisational innovation and social marketing as an example of a business model innovation. Key informants were drawn from non-governmental, government and international organisations using snowball sampling. An article was excluded if the article: did not meet the study's definition of the innovation; did not address dissemination, diffusion, scale up or sustainability of the innovation; did not address low-income or middle-income countries; was superficial in its discussion and/or did not provide empirical evidence about scale-up of the innovation; was not available online in full text; or was not available in English, French, Spanish or Portuguese, resulting in a final sample of 41 peer-reviewed articles and 30 grey literature sources. We used the constant comparative method of qualitative data analysis to extract recurrent themes from the interviews, and we integrated these themes with findings from the

  16. Innovative method for training students to develop enterprising, decision-making and cooperation skills through complex computernetworks team-design

    Directory of Open Access Journals (Sweden)

    Mătăsaru Petre-Daniel

    2017-01-01

    Full Text Available Our paper presents a short overview of the educational methods used in the learning process in telecommunication domain, targeting skills and competences students acquire and develop during the semester and focusing on the ones that are most valuable and appreciated on the labor market after graduation. Our research of the market shows that besides basic engineering knowledge, skills like initiative, team-playing, business analysis, decision-making, marketing and creative project presentation are considered very valuable assets by HR recruiters. We implement and test an improved method that combines classic techniques with the use of modern digital tools, emphasizing on specific tasks that coach the student how to deal with real markets, extract valuable data through analysis, design up-to-date computer-networks, make correlated decisions based also on economic arguments, team working and assume responsibilities. This is achieved through a real-case study and project themes for computer-networks that involve real situations with technical and budgetary challenges, market analysis and research through online facilities and dealing with specialized software for network design and simulation with a creative presentation. The presented approach is intended to prepare faculty staff to implement innovative and self-improving teaching methods in engineering educational process and thus make an educational reform happen [1].

  17. Innovation Online Teaching Module Plus Digital Engineering Kit with Proteus Software through Hybrid Learning Method to Improve Student Skills

    Science.gov (United States)

    Kholis, Nur; Syariffuddien Zuhrie, Muhamad; Rahmadian, Reza

    2018-04-01

    Demands the competence (competence) needs of the industry today is a competent workforce to the field of work. However, during this lecture material Digital Engineering (Especially Digital Electronics Basics and Digital Circuit Basics) is limited to the delivery of verbal form of lectures (classical method) is dominated by the Lecturer (Teacher Centered). Though the subject of Digital Engineering requires learning tools and is required understanding of electronic circuits, digital electronics and high logic circuits so that learners can apply in the world of work. One effort to make it happen is by creating an online teaching module and educational aids (Kit) with the help of Proteus software that can improve the skills of learners. This study aims to innovate online teaching modules plus kits in Proteus-assisted digital engineering courses through hybrid learning approaches to improve the skills of learners. The process of innovation is done by considering the skills and mastery of the technology of students (students) Department of Electrical Engineering - Faculty of Engineering – Universitas Negeri Surabaya to produce quality graduates Use of online module plus Proteus software assisted kit through hybrid learning approach. In general, aims to obtain adequate results with affordable cost of investment, user friendly, attractive and interactive (easily adapted to the development of Information and Communication Technology). With the right design, implementation and operation, both in the form of software both in the form of Online Teaching Module, offline teaching module, Kit (Educational Viewer), and e-learning learning content (both online and off line), the use of the three tools of the expenditure will be able to adjust the standard needs of Information and Communication Technology world, both nationally and internationally.

  18. Growth of ruby crystals by the heat exchanger method, phase 1: NSF small business innovation research

    Science.gov (United States)

    Schmid, F.; Khattak, C. P.

    1980-03-01

    Conditions for the growth of large, uniformly doped laser crystals by the heat exchanger method are explored. Determination of the melt point, selection of crucible material and establishment of furnace operating parameters are discussed. The melt point of ruby was found to be 2040 plus or minus 10 C. Molybdenum crucibles can be used to contain ruby in vacuum as well as under argon atmospheres at desired superheat temperatures over extended periods required for crystal growth. Thermodynamic analysis was conducted and vapor pressures of volatile species calculated. Experimentally, volatilization of chromium oxides was suppressed by using welded covers on crucibles and operating under an argon pressure in the furnace.

  19. A study on total phenolics and vitamin c contents of kalecik karasi (vitis vinifera l.) clones

    International Nuclear Information System (INIS)

    Keskin, N.; Keskin, S.

    2014-01-01

    In this study total phenolic and vitamin C contents of the fully ripe berries of 23 clones of Kalecik Karasi which is one of the leading Turkish local red-wine grape cultivar originally grown in Kizilirmak valley near Kalecik/Ankara region were examined under the clone selection project supported by TUBTAK (Project Nr: 107 O 731). High-pressure liquid chromatography (HPLC) was used for vitamin C and spectrophotometer for total phenolics estimation. One way ANOVA was used to compare means of clone for their total phenolic and vitamin C contents. In addition to this univariate method, hierarchical cluster analysis was performed to identify similarity levels among the clones by considering total phenolics and vitamin C content together. Differences among the clones were found statistically significant for both characteristics. Total phenolic contents of the clones varied from 3.310 mg (clone 21) to 3.389 mg (clone 6) as GAE g fw. Vitamin C content ranged from 14.010 mg (clone 6) to 16.500 mg (clone 19) in 100g fw. Furthermore, similarity level for all clones was 83.1% that means variation rate is about 17% among the clones. As a summary of whole data, the first three performing clones are 6 (3.389 mg), 10 (3.374 mg) and 1 (3.365 mg) for total phenolics, and 19 (16.500 mg), 9 (16.020 mg), and 21 (16.015 mg) for vitamin C contents of the berries. (author)

  20. Methods of technical and tactical training basketball players with hearing impairments using innovative technologies

    Directory of Open Access Journals (Sweden)

    Zh.L Kozina

    2014-06-01

    Full Text Available Purpose: to develop information technology for technical and tactical training of skilled basketball players with hearing impairments. Material : the study involved 24 women's national team athletes Ukrainian Basketball hearing impaired (age - 25-30 years. Technical protocols were processed 12 games from the World Cup and Europe, XXII Summer Deaflympics. Fixed number of shots and hit free throws, steals, rebounds and on its offensive rebounds, fouls and errors. Results : the developed system of tactical training basketball players with hearing impairments. Accentuation was made of the prevalence of method visibility while developing tactical interactions. The main result was the development of the author's method of video tutorials with animated illustrations. Also, use the lamp LS Line-3-65-12-C to control the training process athletes. Conclusions : there was a significant improvement in competitive activity athletes. Athletes performed significantly more hits from the foul line and successful shots from long range compared to main rivals. Also increased the number of interceptions in games. Application of information technology had an impact on the competitive result: basketball team of Ukraine took the second place in the Deaflympics in Sofia in 2013.

  1. An innovative method for the solidification/stabilization of PAHs-contaminated soil using sulfonated oil.

    Science.gov (United States)

    Ma, Fujun; Wu, Bin; Zhang, Qian; Cui, Deshan; Liu, Qingbing; Peng, Changsheng; Li, Fasheng; Gu, Qingbao

    2018-02-15

    Stabilization/solidification (S/S) has been successfully employed in many superfund sites contaminated with organic materials. However, this method's long-term effectiveness has not been fully evaluated and the increase in soil volume following treatment is unfavorable to follow-up disposal. The present study developed a novel method for the S/S of PAHs-contaminated soil with the facilitation of sulfonated oil (SO). Adding SO significantly improved the unconfined compressive strength (UCS) values of Portland cement and activated carbon (PC-AC) treated soil samples, and the UCS values of the soil sample treated with 0.02% of SO were up to 2.3 times higher than without SO addition. When the soil was treated with PC-AC-SO, the PAHs leaching concentrations were 14%-25% of that in leachates of the control soil, and high molecular weight PAHs including benzo(a)pyrene were rarely leached. Freeze/thaw durability tests reveal that the leachability of PAHs was not influenced by freeze-thaw cycles. The UCS values of PC-AC-SO treated soil samples were 2.2-3.4 times greater than those of PC-AC treated soil samples after 12 freeze-thaw cycles. The PC-AC-SO treated soils resist disintegration better when compared to the PC-AC treated soils. The SEM micrographs reveal that the soils' compactness was significantly improved when treated with SO. Copyright © 2017 Elsevier B.V. All rights reserved.

  2. Experimental reversion of the optimal quantum cloning and flipping processes

    International Nuclear Information System (INIS)

    Sciarrino, Fabio; Secondi, Veronica; De Martini, Francesco

    2006-01-01

    The quantum cloner machine maps an unknown arbitrary input qubit into two optimal clones and one optimal flipped qubit. By combining linear and nonlinear optical methods we experimentally implement a scheme that, after the cloning transformation, restores the original input qubit in one of the output channels, by using local measurements, classical communication, and feedforward. This nonlocal method demonstrates how the information on the input qubit can be restored after the cloning process. The realization of the reversion process is expected to find useful applications in the field of modern multipartite quantum cryptography

  3. Medical innovation laws: an unnecessary innovation.

    Science.gov (United States)

    Richards, Bernadette

    2016-06-01

    Objective This paper aims to demonstrate that any suggestion that there is a need for specific innovation laws is flawed. Innovation is central to good medical practice and is adequately supported by current law. Methods The paper reviews the nature of medical innovation and outlines recent attempts in the UK to introduce specific laws aimed at 'encouraging' and 'supporting' innovation. The current legal framework is outlined and the role of the law in relation to medical innovation explored. Results The analysis demonstrates the cyclic relationship between medical advancement and the law and concludes that there is no requirement for specific innovation laws. Conclusions The law not only supports innovation and development in medical treatment but encourages it as central to a functioning medical system. There is no need to introduce specific laws aimed at medical innovation; to do so represents an unnecessary legal innovation and serves to complicate matters. What is known about the topic? Over recent months, there has been a great deal of discussion surrounding the law in the context of medical innovation. This was driven by the attempts in the UK to introduce specific laws in the Medical Innovation Bill. The general subject matter - negligence and the expected standard of care in the provision of treatment - is very well understood, but not in cases where the treatment can be described as innovative. The general rhetoric in both the UK and Australia around the Medical Innovation Bill demonstrates a lack of understanding of the position of the law with regards to innovative treatment. What does this paper add? This paper adds clarity to the debate. It presents the law and explains the manner in which the law can operate around innovative treatment. The paper asserts that medical innovation is both supported and encouraged by existing legal principles. What are the implications for practitioners? The paper presents an argument that can guide the policy position

  4. 'Twin2twin' an innovative method of empowering midwives to strengthen their professional midwifery organisations.

    Science.gov (United States)

    Cadée, Franka; Perdok, Hilde; Sam, Betty; de Geus, Myrte; Kweekel, Liselotte

    2013-10-01

    midwives need professional support from a national midwifery organisation to be able to provide the services that are by regulatory mechanisms and accreditation expected of them. Not all midwives in the world are united in a professional organisation. The aim of this project was to strengthen the midwifery organisations of Sierra Leone and the Netherlands. During the process of the project it was realised that the development of a platform of exchange at organisational level would be enhanced by introducing personal exchange between individual midwives. In response to this new insight the original project plan was adjusted by incorporating the twin2twin method. twin2twin is a feminist methodology of mutual exchange between twenty pairs of midwives from different organisations (in this case Sierra Leone and the Netherlands). The method can be distinguished by 10 specific steps. It was developed, used and (re)evaluated through focus group discussions, storytelling and written evaluations. twinning of organisations was strengthened by adding a human component to the process. With the use of the 'twin2twin' method, midwives were encouraged to invested in a professional and personal bond with their 'twin sister'. This bond was independent and went beyond the relatively short four year project period. Through personal engagement and mutual exchange of knowledge and skills, midwives empowered each other to build and strengthen their midwifery organisations both in Sierra Leone and the Netherlands. (Empowerment refers to the expansion in people's ability to make strategic life choices in a context where this ability was previously denied to them (Narayan, 2005); organisational empowerment includes processes and structures that enhance members' skills and provides them with the mutual support necessary to effect community level change (Zimmerman, 1995).). despite challenges we are convinced that twin2twin can be of additional benefit for the success of other projects

  5. Data Mining – Innovative Method for Obtaining Information in Marketingand Business Management

    Directory of Open Access Journals (Sweden)

    Mirela-Cristina Voicu

    2011-05-01

    Full Text Available The existence of massive amounts of data raised the question of using their reorientation to a retrospective to a prospective operation. Data mining offers the promise of an important aid for discovering hidden patterns in data that can be used to predict the behavior of customers, products and processes. Data mining tools must be guided by users who understand the business, the general nature of the data and analytical methods involved. It discovers information within the data that queries and reports can’t effectively reveal. It is vital to collect data and prepare properly, to face reality models. Choosing the most appropriate product data mining is to find a tool with the capabilities required, an interface that matches the skills of users and can be applied in a specific business problem. In this context, the purpose of this paper is to illustrate some of the problems of company activity problems which can be solved by using data mining techniques.

  6. Innovative Methods of Anthropogenic Landscape Reconstruction in the Urbanized Oil and Gas Region Environment

    Science.gov (United States)

    Shabatura, L. N.; Bauer, N. V.; Speranskaya, N. I.; Iatsevich, O. E.

    2016-10-01

    The article deals with the problems of the urbanized environment appearing as a result of intensive region developing. The state neglect towards people affects the population life quality of the of oil and gas extraction areas as well as problems resolving, and it provokes enormous losses for manufacturing and the whole region. The environment influences the person behaviour, one's perception and space understanding. The city environment is considered as the human existence space influencing on it directly, so it is necessary to renovate it. The authentic region developing cannot be reduced to "pure" economics (for not to be deserted), but needs to be fully mastered. To renovate the destroyed landscapes, it is necessary to use the landscape design methods making a cultural landscape. They help to increase the natural components of the city environment and to make it more harmonious, more harmless, more comfortable for residents.

  7. Drying of water based foundry coatings: Innovative test, process design and optimization methods

    DEFF Research Database (Denmark)

    Di Muoio, Giovanni Luca; Johansen, Bjørn Budolph

    on real industrial cases. These tools have been developed in order to simulate and optimize the drying process and reduce drying time and power consumption as well as production process design time and cost of expensive drying equipment. Results show that test methods from other industries can be used...... capacity goals there is a need to understand how to design, control and optimize drying processes. The main focus of this project was on the critical parameters and properties to be controlled in production in order to achieve a stable and predictable drying process. We propose for each of these parameters...... of Denmark with the overall aim to optimize the drying process of water based foundry coatings. Drying of foundry coatings is a relatively new process in the foundry industry that followed the introduction of water as a solvent. In order to avoid moisture related quality problems and reach production...

  8. Innovative methods for the identification of predictive biomarker signatures in oncology: Application to bevacizumab

    Directory of Open Access Journals (Sweden)

    Paul Delmar

    2017-03-01

    Full Text Available Current methods for subgroup analyses of data collected from randomized clinical trials (RCTs may lead to false-positives from multiple testing, lack power to detect moderate but clinically meaningful differences, or be too simplistic in characterizing patients who may benefit from treatment. Herein, we present a general procedure based on a set of newly developed statistical methods for the identification and evaluation of complex multivariate predictors of treatment effect. Furthermore, we implemented this procedure to identify a subgroup of patients who may receive the largest benefit from bevacizumab treatment using a panel of 10 biomarkers measured at baseline in patients enrolled on two RCTs investigating bevacizumab in metastatic breast cancer. Data were collected from patients with human epidermal growth factor receptor 2 (HER2-negative (AVADO and HER2-positive (AVEREL metastatic breast cancer. We first developed a classification rule based on an estimated individual scoring system, using data from the AVADO study only. The classification rule takes into consideration a panel of biomarkers, including vascular endothelial growth factor (VEGF-A. We then classified the patients in the independent AVEREL study into patient groups according to “promising” or “not-promising” treatment benefit based on this rule and conducted a statistical analysis within these subgroups to compute point estimates, confidence intervals, and p-values for treatment effect and its interaction. In the group with promising treatment benefit in the AVEREL study, the estimated hazard ratio of bevacizumab versus placebo for progression-free survival was 0.687 (95% confidence interval [CI]: 0.462–1.024, p = 0.065, while in the not-promising group the hazard ratio (HR was 1.152 (95% CI: 0.526–2.524, p = 0.723. Using the median level of VEGF-A from the AVEREL study to divide the study population, then the HR becomes 0.711 (95% CI: 0.435–1.163, p = 0

  9. Technologic advances in aural rehabilitation: applications and innovative methods of service delivery.

    Science.gov (United States)

    Sweetow, Robert W; Sabes, Jennifer Henderson

    2007-06-01

    The level of interest in aural rehabilitation has increased recently, both in clinical use and in research presentations and publications. Advances in aural rehabilitation have seen previous techniques such as speech tracking and analytic auditory training reappear in computerized forms. These new delivery methods allow for a consistent, cost-effective, and convenient training program. Several computerized aural rehabilitation programs for hearing aid wearers and cochlear implant recipients have recently been developed and were reported on at the 2006 State of the Science Conference of the Rehabilitation Engineering Research Center on Hearing Enhancement at Gallaudet University. This article reviews these programs and outlines the similarities and differences in their design. Another promising area of aural rehabilitation research is the use of pharmaceuticals in the rehabilitation process. The results from a study of the effect of d-amphetamine in conjunction with intensive aural rehabilitation with cochlear implant patients are also described.

  10. Innovative method for optimizing Side-Scan Sonar mapping: The blind band unveiled

    Science.gov (United States)

    Pergent, Gérard; Monnier, Briac; Clabaut, Philippe; Gascon, Gilles; Pergent-Martini, Christine; Valette-Sansevin, Audrey

    2017-07-01

    Over the past few years, the mapping of Mediterranean marine habitats has become a priority for scientists, environment managers and stakeholders, in particular in order to comply with European directives (Water Framework Directive and Marine Strategy Framework Directive) and to implement legislation to ensure their conservation. Side-scan sonar (SSS) is recognised as one of the most effective tool for underwater mapping. However, interpretation of acoustic data (sonograms) requires extensive field calibration and the ground-truthing process remains essential. Several techniques are commonly used, with sampling methods involving grabs, scuba diving observations or Remotely Operated Vehicle (ROV) underwater video recordings. All these techniques are time consuming, expensive and only provide sporadic informations. In the present study, the possibility of coupling a camera with a SSS and acquiring underwater videos in a continuous way has been tested. During the 'PosidCorse' oceanographic survey carried out along the eastern coast of Corsica, optical and acoustic data were respectively obtained using a GoPro™ camera and a Klein 3000™ SSS. Thereby, five profiles were performed between 10 and 50 m depth, corresponding to more than 20 km of data acquisition. The vertical images recorded with the camera fixed under the SSS and positioned facing downwards provided photo mosaics of very good quality corresponding to the entire sonograms's blind band. From the photo mosaics, 94% of the different bottom types and main habitats have been identified; specific structures linked to hydrodynamics conditions, anthropic and biological activities have also been observed as well as the substrate on which the Posidonia oceanica meadow grows. The association between acoustic data and underwater videos has proved to be a non-destructive and cost-effective method for ground-truthing in marine habitats mapping. Nevertheless, in order to optimize the results over the next surveys

  11. Low Temperature Two-Steps Sintering (LTTSS) - an innovative method for consolidating porous UO2 pellets

    International Nuclear Information System (INIS)

    Sanjay Kumar, D.; Ananthasivan, K.; Senapati, Abhiram; Venkata Krishnan, R.

    2015-01-01

    Metallic uranium and its alloys are an important fuel for fast reactors. Presently, metallic uranium is being prepared using expensive fluoro-metallothermic process. Recent reports suggest that metal oxide could be reduced to metal using a novel electrochemical de-oxidation method and this could serve as attractive alternate for expensive metallothermic process. In view of which, a research program is being pursued in our Centre to develop an optimum process parameter for the scaled up preparation of metallic uranium efficiently. One of the important process parameter is the size, nature and distribution of porosity in the urania pellet. Essentially the ceramic form of the urania should encompass interconnected porosity that would allow percolation of melts into the UO 2 . However, the matrix density of the pellet should be high to ensure that it possesses good handling strength and is electrically conducting. Hence preparation of high dense porous UO 2 pellets was required. In this study, we report the preparation of porous UO 2 pellets possessing a very high matrix density by using the citrate gel-combustion method. The 'as-prepared' powders were consolidated at various compaction pressures as such and these pellets were sintered in 8 mol %Ar+H 2 gas with a flow rate of 250 mL/min at 1073 K for 30 min followed by soaking at 1473 K for 4 h with heating rate of 5 K min -1 in a molybdenum furnace. X-ray diffraction studies revealed that these pellets contained UO 2 . The morphological analysis sintered pellets was carried out by using Scanning Electron Microscope (M/s. Philips model XL 30, Netherlands). All these pellets were gold coated

  12. (PCR) for direct cloning of blunt-end DNA fragments

    African Journals Online (AJOL)

    Administrator

    2011-09-19

    Sep 19, 2011 ... Key words: Blunt-end cloning, phosphorylated DNA fragment, dephosphorylated blunt-end vector. INTRODUCTION ... With this method, a lot of steps are saved, which includes restriction .... pBSK-blunt (data not shown).

  13. An innovative method for automatic determination of time of arrival for Lamb waves excited by impact events

    Science.gov (United States)

    Zhu, Junxiao; Parvasi, Seyed Mohammad; Ho, Siu Chun Michael; Patil, Devendra; Ge, Maochen; Li, Hongnan; Song, Gangbing

    2017-05-01

    Lamb waves have great potential as a diagnostic tool in the application of structural health monitoring. Propagation properties of Lamb waves are affected by the state of the structure that the waves are traveling upon. Thus Lamb waves can carry information about the structure as they travel across a structure. However, the dispersive, multimodal and attenuation characteristics of Lamb waves make it difficult to determine the time of arrival of Lamb waves. To deal with these characteristics, an innovative method to automatically determine the time of arrival for impact-induced Lamb waves without human intervention is proposed in this paper. Lead zirconate titanate sensors mounted on the surface of an aluminum plate were used to measure the Lamb waves excited by an impact. The time of arrival was determined based on wavelet decomposition, Hilbert transform and statistics (Grubbs’ test and maximum likelihood estimation). Both of numerical analysis and physical measurements have verified the accuracy of this method for impacts on an aluminum plate.

  14. Innovations in electrophoretic deposition: Alternating current and pulsed direct current methods

    International Nuclear Information System (INIS)

    Chávez-Valdez, Alejandra; Boccaccini, Aldo R.

    2012-01-01

    This review summarizes emerging developments in the field of alternating current (AC) and pulsed direct current (DC) electrophoretic deposition (EPD) in aqueous or organic media. Numerous applications of AC-EPD are discussed including two major groups of investigations: (i) AC-EPD to suppress water hydrolysis at high voltages in inorganic (ceramic) coatings and (ii) AC-EPD for deposition of biological entities. The deposition, purification and manipulation of carbon nanotubes and nanoparticles by AC-EPD to form specific arrays, for development of sensors and other electronic devices and the application of AC-EPD as method for separation of particles according to their shape or size are also presented. Other applications reviewed relate to the fabrication by AC-EPD of toxic gas sensors from oxides and superconducting layers. The main materials being examined by AC-EPD are inorganic, including carbon nanotubes, TiO 2 nanoparticles, Al 2 O 3 , Si, SnO 2 , ZnO and WO 3 and biological entities, e.g. bacteria cells. For pulsed EPD, the applications reviewed are divided in pulsed current and pulsed voltage EPD. Among the applications of pulsed EPD, the formation of thick films from aqueous suspensions without water decomposition, the fabrication of multilayer and composite materials and the size-selective deposition of ceramic nanoparticles are the most important investigated to date, based on the quality of the coatings and deposits obtained and their relevance for applications.

  15. [The discrete horror of cloning].

    Science.gov (United States)

    Guibourg, Ricardo A

    2009-01-01

    The author raises the topic of cloning after the decision of the Argentine government, which concerned for the "dignity of the human person", passed a decree of need and urgency, No. 200/97 (Annex), prohibiting cloning experiments with human beings. Therefore, considering that the topic is so terribly urgent and necessary, the author feels it is timely to consider it.

  16. Innovative methods for the design of miniatured state of the art neutronic channels for future FBR

    International Nuclear Information System (INIS)

    Sivaramakrishna, M.; Shukla, Rahul; Nagaraj, C.P.; Madhusoodanan, K.

    2011-01-01

    Neutron flux in fast reactor varies over 10 decades from shutdown to full power. This is monitored in all states of the reactor by Neutron flux monitoring systems. The system consists of several sets of detectors and instrument channels. For smooth transition from one set of instrument channel to other, interlocks with auto inhibition in safety logic are provided. In the present reactor, Fission counters are placed in control plug under vessel. The signals from these detectors are connected to preamplifiers near to the sensors. The signals from these preamplifiers are again connected to instrument channels in control building local control centres, which generate parameters such as logarithmic power, period, linear power and reactivity signals. Interlocks with auto inhibition are provided in safety logic for smooth transition from one detector-channel to other. With this arrangement, difficulties are faced such as, two sets of detectors are required at two different locations. Three different types of instrument channels are required. In each channel, there is a trade-off between response time and accuracy. Many interlocks with auto inhibitions are to be provided for transition from one system to another. Volume of electronics involved is very high. The pulses are also not periodical. Hence counting techniques do not result in accurate prediction of frequency and intern power. Either the accuracy of counting or the counting time has to be sacrificed. It can also be seen that as the frequency goes up the pulses over lap. Hence estimating the power using pulse counting can't predict the power correctly. In the Campbell method, which is used in the intermediate range also there is a trade-off between the accuracy and response time and linearity could be seen only for 3 decades. Hence the new approach viz. PCA (principle component analysis) is discussed in this paper. PCA is non-parametric method of extracting relevant information from mixed data by reducing a complex

  17. Orchestrating innovation

    OpenAIRE

    Berkers, F.T.H.M.; Klein Woolthuis, R.J.A.; Boer, J. de

    2015-01-01

    Orchestrating Innovation increases the probability of success, minimizing the probability of failure of technological innovations by creating sustained societal and economic value. Orchestrating innovation propagates to take into account and actively involve all relevant stakeholders of the (future) ecosystem in which the innovation will, can or has to be adopted.

  18. Mindful innovation

    DEFF Research Database (Denmark)

    Olsen, Poul Bitsch

    2008-01-01

    Mindful innovation is an approach to innovation that pays attention to people's experience in an organization rather than to formal organization or social role.......Mindful innovation is an approach to innovation that pays attention to people's experience in an organization rather than to formal organization or social role....

  19. Innovative didaktik

    DEFF Research Database (Denmark)

    Lund, Birthe

    Innovative didaktik. This deals with innovative didaktik from at methodological point of view in three ways - how to define the concept, how to develop it and how analyse it. Issues analysed: How to create innovative students? How to create innovative learning envoriments? These are core question...... in "Projekt Innovativ didaktik". The hidden curriculum is to inspire students to develop entreprenuership and creativty....

  20. Animal cloning: problems and prospects.

    Science.gov (United States)

    Wells, D N

    2005-04-01

    An efficient animal cloning technology would provide many new opportunities for livestock agriculture, human medicine, and animal conservation. Nuclear cloning involves the production of animals that are genetically identical to the donor cells used in a technique known as nuclear transfer (NT). However, at present it is an inefficient process: in cattle, only around 6% of the embryos transferred to the reproductive tracts of recipient cows result in healthy, longterm surviving clones. Of concern are the high losses throughout gestation, during birth and in the post-natal period through to adulthood. Many of the pregnancy losses relate to failure of the placenta to develop and function correctly. Placental dysfunction may also have an adverse influence on postnatal health. These anomalies are probably due to incorrect epigenetic reprogramming of the donor genome following NT, leading to inappropriate patterns of gene expression during the development of clones. Whilst some physiological tests on surviving clones suggest normality, other reports indicate a variety of post-natal clone-associated abnormalities. This variability in outcome may reflect species-specific and/or cloning methodological differences. Importantly, to date it appears that these clone-associated phenotypes are not transmitted to offspring following sexual reproduction. This indicates that they represent epigenetic errors, rather than genetic errors, which are corrected during gametogenesis. Whilst this needs confirmation at the molecular level, it provides initial confidence in the first application of NT in agriculture, namely, the production of small numbers of cloned sires from genetically elite bulls, for natural mating, to effectively disseminate genetic gain. In addition to the animal welfare concerns with the technology, the underlying health of the animals and the consequential effect on food safety are critical aspects that require investigation to gain regulatory and consumer

  1. Human cloning: can it be made safe?

    Science.gov (United States)

    Rhind, Susan M; Taylor, Jane E; De Sousa, Paul A; King, Tim J; McGarry, Michelle; Wilmut, Ian

    2003-11-01

    There are continued claims of attempts to clone humans using nuclear transfer, despite the serious problems that have been encountered in cloning other mammals. It is known that epigenetic and genetic mechanisms are involved in clone failure, but we still do not know exactly how. Human reproductive cloning is unethical, but the production of cells from cloned embryos could offer many potential benefits. So, can human cloning be made safe?

  2. Innovative Extraction Method for a Coal Seam with a Thick Rock-Parting for Supporting Coal Mine Sustainability

    Directory of Open Access Journals (Sweden)

    Meng Li

    2017-10-01

    Full Text Available As thick rock partings delay the efficient mining of coal seams and constrain the sustainable development of coal mines, an innovative extraction method for a coal seam with thick rock parting was proposed. The coal seams were divided into different sub-zones according to the thickness of rock parting and then the sub-zones were mined by separately using three mining schemes involving full-seam mining, combined mining using backfill and caving (CMBC, and reducing height mining. Afterwards, the study introduced the basic mechanism and key devices for the CMBC and analysed the working state of the backfill support in detail. Moreover, the method for calculating the length of the backfill zone was proposed to design the length of backfill zone and the influences of four factors (including bulking coefficient of rock parting on the length of the backfill zone were also explored. By taking the No. 22203 panel, Buertai mine, Inner Mongolia, China as an example, the mined coal resource by using the CMBC extraction method will increase by 1.83 × 106 tons and the recovery ratio will rise from 56.2% to 92.4% compared with mining of the 2-2 upper coal seam alone. Moreover, by applying CMBC, a series of environmental and ecological problems caused by rock parting is reduced, which can improve the environment in mined areas. The research can provide technological guidance for mining panels of a coal seam with a thick rock parting and the disposal thereof under similar conditions.

  3. DESI-MS2: a rapid and innovative method for trace analysis of six cytostatic drugs in health care setting.

    Science.gov (United States)

    Fabrizi, Giovanni; Fioretti, Marzia; Rocca, Lucia Mainero; Curini, Roberta

    2012-05-01

    With the aim of establishing exposure levels for hospital personnel preparing and administering cytostatic drugs (CDs), here, we present an innovative screening method based on the use of the desorption electrospray ionization (DESI) interface coupled with a hybrid quadrupole linear ion trap mass spectrometer. A rapid, simple, and sensitive procedure was developed for the simultaneous surface monitoring of cyclophosphamide, dacarbazine, methotrexate, vincristine, gemcitabine, and cytarabine. Since analytes were in the solid state, a novel approach based on the use of passive samplers was combined with the direct analysis of wipes. A PTFE-printed glass slide was used as a passive sampler, while hydrophobic centers of Swiffer® cloths were judged extremely efficient as wipe samplers. After the sampling period, the CD collectors were directly processed with the DESI-MS system without any further treatment. MS/MS confirmatory analysis was conducted using selected reaction monitoring in the positive ion mode and detection limits were evaluated. Values were at the picograms per square millimeter levels on the passive collector and at the picograms per square centimeter levels for the wipe ones. Direct determination on solid-state samples combined with mass spectrometry selectivity provided a powerful tool so far unapplied to occupational hygiene.

  4. Efficiency assessment method of financial leasing as a factor of innovative development of a construction complex for a lessee in comparison with a credit

    Directory of Open Access Journals (Sweden)

    Alekseeva Tat’yana Romanovna

    Full Text Available Modernization and innovative and technological rearmament of a construction complex is one of the priority problems of national economy development. Development and implementation of innovative technologies in the process of creating construction production will allow improving its quality, consumer characteristics, ensuring energy efficiency and ecological safety of buildings and constructions. One of problems of innovative development of a construction complex is the problem of financing of innovative activity. In our opinion leasing is one of effective ways of its solving. In the leasing transaction the owner of an asset temporarily transfers a right to use an asset to other party. The owner of an asset is a lessor. Other party is a lessee. The lessor makes a lease for a specified time in return for a periodic rental payments from the lessee. One of the advantages of leasing is that it provides alternative to ownership. Also lessees benefit from a number of tax advantages. Leasing has many other advantages. The assessment method of the efficiency of financial leasing as a factor of innovative development of a construction complex for a lessee enterprise in comparison with the credit is shown in article. As a result of scientific research we specified the criteria of this assessment.

  5. Wildlife conservation and reproductive cloning.

    Science.gov (United States)

    Holt, William V; Pickard, Amanda R; Prather, Randall S

    2004-03-01

    Reproductive cloning, or the production of offspring by nuclear transfer, is often regarded as having potential for conserving endangered species of wildlife. Currently, however, low success rates for reproductive cloning limit the practical application of this technique to experimental use and proof of principle investigations. In this review, we consider how cloning may contribute to wildlife conservation strategies. The cloning of endangered mammals presents practical problems, many of which stem from the paucity of knowledge about their basic reproductive biology. However, situations may arise where resources could be targeted at recovering lost or under-represented genetic lines; these could then contribute to the future fitness of the population. Approaches of this type would be preferable to the indiscriminate generation of large numbers of identical individuals. Applying cloning technology to non-mammalian vertebrates may be more practical than attempting to use conventional reproductive technologies. As the scientific background to cloning technology was pioneered using amphibians, it may be possible to breed imminently threatened amphibians, or even restore extinct amphibian species, by the use of cloning. In this respect species with external embryonic development may have an advantage over mammals as developmental abnormalities associated with inappropriate embryonic reprogramming would not be relevant.

  6. YAC clone information - RGP physicalmap | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available 08/lsdba.nbdc00318-06-002 Description of data contents YAC clones selected with DNA markers Data file File name: rgp_physical...map_yac_clones.zip File URL: ftp://ftp.biosciencedbc.jp/archive/rgp-physicalmap/LATEST/rgp_physical...sciencedbc.jp/togodb/view/rgp_physicalmap_yac_clones#en Data acquisition method YAC clones selected with RGP...rom. No. Chromosome number Region Region number Physical map image The file name of rice physical map Order ...bout This Database Database Description Download License Update History of This Database Site Policy | Contact Us YAC clone information - RGP physicalmap | LSDB Archive ...

  7. Evaluation of innovation processes

    Directory of Open Access Journals (Sweden)

    Jakub Tabas

    2012-01-01

    Full Text Available In present, innovations are spoken as an engine of the world economy because the innovations are transforming not only business entities but the whole industries. The innovations have become a necessity for business entities in order to survive on floating challenging markets. This way, innovations are driving force of companies’ performance. The problem which arises here is a question of measurement innovation’s effect on the financial performance of company or selection between two or more possible variants of innovation’s realization. Various authors which are focused on innovations processes are divided into two groups in their attitudes towards the question of influence of innovations on financial performance of companies. One group of the authors present the idea that any reliable measurement is not possible or efficient. The second group of authors present some methods theoretically applicable on this measurement but they base their approaches mostly on the methods of measurement of investments effectiveness or they suggest employment of indicators or ratios which wouldn’t be clearly connected with the outcome of innovation process. The aim of submitted article is to compare different approaches to evaluation of the innovation processes. The authors compare various approaches here and by use of analysis and synthesis, they determine their own method how to measure outcome of innovation process.

  8. Computational fluid dynamics analysis of an innovative start-up method of high temperature fuel cells using dynamic 3d model

    Directory of Open Access Journals (Sweden)

    Kupecki Jakub

    2017-03-01

    Full Text Available The article presents a numerical analysis of an innovative method for starting systems based on high temperature fuel cells. The possibility of preheating the fuel cell stacks from the cold state to the nominal working conditions encounters several limitations related to heat transfer and stability of materials. The lack of rapid and safe start-up methods limits the proliferation of MCFCs and SOFCs. For that reason, an innovative method was developed and verified using the numerical analysis presented in the paper. A dynamic 3D model was developed that enables thermo-fluidic investigations and determination of measures for shortening the preheating time of the high temperature fuel cell stacks. The model was implemented in ANSYS Fluent computational fluid dynamic (CFD software and was used for verification of the proposed start-up method. The SOFC was chosen as a reference fuel cell technology for the study. Results obtained from the study are presented and discussed.

  9. Therapeutic cloning in the mouse

    Science.gov (United States)

    Mombaerts, Peter

    2003-01-01

    Nuclear transfer technology can be applied to produce autologous differentiated cells for therapeutic purposes, a concept termed therapeutic cloning. Countless articles have been published on the ethics and politics of human therapeutic cloning, reflecting the high expectations from this new opportunity for rejuvenation of the aging or diseased body. Yet the research literature on therapeutic cloning, strictly speaking, is comprised of only four articles, all in the mouse. The efficiency of derivation of embryonic stem cell lines via nuclear transfer is remarkably consistent among these reports. However, the efficiency is so low that, in its present form, the concept is unlikely to become widespread in clinical practice. PMID:12949262

  10. Human therapeutic cloning (NTSC): applying research from mammalian reproductive cloning.

    Science.gov (United States)

    French, Andrew J; Wood, Samuel H; Trounson, Alan O

    2006-01-01

    Human therapeutic cloning or nuclear transfer stem cells (NTSC) to produce patient-specific stem cells, holds considerable promise in the field of regenerative medicine. The recent withdrawal of the only scientific publications claiming the successful generation of NTSC lines afford an opportunity to review the available research in mammalian reproductive somatic cell nuclear transfer (SCNT) with the goal of progressing human NTSC. The process of SCNT is prone to epigenetic abnormalities that contribute to very low success rates. Although there are high mortality rates in some species of cloned animals, most surviving clones have been shown to have normal phenotypic and physiological characteristics and to produce healthy offspring. This technology has been applied to an increasing number of mammals for utility in research, agriculture, conservation, and biomedicine. In contrast, attempts at SCNT to produce human embryonic stem cells (hESCs) have been disappointing. Only one group has published reliable evidence of success in deriving a cloned human blastocyst, using an undifferentiated hESC donor cell, and it failed to develop into a hESC line. When optimal conditions are present, it appears that in vitro development of cloned and parthenogenetic embryos, both of which may be utilized to produce hESCs, may be similar to in vitro fertilized embryos. The derivation of ESC lines from cloned embryos is substantially more efficient than the production of viable offspring. This review summarizes developments in mammalian reproductive cloning, cell-to-cell fusion alternatives, and strategies for oocyte procurement that may provide important clues facilitating progress in human therapeutic cloning leading to the successful application of cell-based therapies utilizing autologous hESC lines.

  11. Innovation in practice, dialogue

    DEFF Research Database (Denmark)

    Reinhard, CarrieLynn D.

    Presentation at the March 19, 2010 project meeting for the Virtual Worlds Research Group.  This presentation is the first report of research conducted in June, 2009, in how the Danish firm Innovation Lab makes sense of innovation as it relates to virtual world technologies.  The method used in th...... in this study was the focus group, modified using Dervin's Sense-Making Methodology.  This presentation focuses on how the members of Innovation Lab defined what is innovation, with discussion on whether or not having a unified definition within the organization makes the most sense....

  12. Essence: Facilitating Software Innovation

    DEFF Research Database (Denmark)

    Aaen, Ivan

    2008-01-01

      This paper suggests ways to facilitate creativity and innovation in software development. The paper applies four perspectives – Product, Project, Process, and People –to identify an outlook for software innovation. The paper then describes a new facility–Software Innovation Research Lab (SIRL......) – and a new method concept for software innovation – Essence – based on views, modes, and team roles. Finally, the paper reports from an early experiment using SIRL and Essence and identifies further research....

  13. Evaluation of a pooled strategy for high-throughput sequencing of cosmid clones from metagenomic libraries.

    Science.gov (United States)

    Lam, Kathy N; Hall, Michael W; Engel, Katja; Vey, Gregory; Cheng, Jiujun; Neufeld, Josh D; Charles, Trevor C

    2014-01-01

    High-throughput sequencing methods have been instrumental in the growing field of metagenomics, with technological improvements enabling greater throughput at decreased costs. Nonetheless, the economy of high-throughput sequencing cannot be fully leveraged in the subdiscipline of functional metagenomics. In this area of research, environmental DNA is typically cloned to generate large-insert libraries from which individual clones are isolated, based on specific activities of interest. Sequence data are required for complete characterization of such clones, but the sequencing of a large set of clones requires individual barcode-based sample preparation; this can become costly, as the cost of clone barcoding scales linearly with the number of clones processed, and thus sequencing a large number of metagenomic clones often remains cost-prohibitive. We investigated a hybrid Sanger/Illumina pooled sequencing strategy that omits barcoding altogether, and we evaluated this strategy by comparing the pooled sequencing results to reference sequence data obtained from traditional barcode-based sequencing of the same set of clones. Using identity and coverage metrics in our evaluation, we show that pooled sequencing can generate high-quality sequence data, without producing problematic chimeras. Though caveats of a pooled strategy exist and further optimization of the method is required to improve recovery of complete clone sequences and to avoid circumstances that generate unrecoverable clone sequences, our results demonstrate that pooled sequencing represents an effective and low-cost alternative for sequencing large sets of metagenomic clones.

  14. Cloning of a quantum measurement

    International Nuclear Information System (INIS)

    Bisio, Alessandro; D'Ariano, Giacomo Mauro; Perinotti, Paolo; Sedlak, Michal

    2011-01-01

    We analyze quantum algorithms for cloning of a quantum measurement. Our aim is to mimic two uses of a device performing an unknown von Neumann measurement with a single use of the device. When the unknown device has to be used before the bipartite state to be measured is available we talk about 1→2 learning of the measurement, otherwise the task is called 1→2 cloning of a measurement. We perform the optimization for both learning and cloning for arbitrary dimension d of the Hilbert space. For 1→2 cloning we also propose a simple quantum network that achieves the optimal fidelity. The optimal fidelity for 1→2 learning just slightly outperforms the estimate and prepare strategy in which one first estimates the unknown measurement and depending on the result suitably prepares the duplicate.

  15. Human cloning and 'posthuman' society.

    Science.gov (United States)

    Blackford, Russell

    2005-01-01

    Since early 1997, when the creation of Dolly the sheep by somatic cell nuclear transfer was announced in Nature, numerous government reports, essays, articles and books have considered the ethical problems and policy issues surrounding human reproductive cloning. In this article, I consider what response a modern liberal society should give to the prospect of human cloning, if it became safe and practical. Some opponents of human cloning have argued that permitting it would place us on a slippery slope to a repugnant future society, comparable to that portrayed in Aldous Huxley's novel, Brave New World. I conclude that, leaving aside concerns about safety, none of the psychological or social considerations discussed in this article provides an adequate policy justification for invoking the state's coercive powers to prevent human cloning.

  16. Cloning of a quantum measurement

    Energy Technology Data Exchange (ETDEWEB)

    Bisio, Alessandro; D' Ariano, Giacomo Mauro; Perinotti, Paolo; Sedlak, Michal [QUIT Group, Dipartimento di Fisica ' ' A. Volta' ' and INFN, via Bassi 6, I-27100 Pavia (Italy); QUIT Group, Dipartimento di Fisica ' ' A. Volta' ' via Bassi 6, I-27100 Pavia (Italy) and Institute of Physics, Slovak Academy of Sciences, Dubravska cesta 9, SK-845 11 Bratislava (Slovakia)

    2011-10-15

    We analyze quantum algorithms for cloning of a quantum measurement. Our aim is to mimic two uses of a device performing an unknown von Neumann measurement with a single use of the device. When the unknown device has to be used before the bipartite state to be measured is available we talk about 1{yields}2 learning of the measurement, otherwise the task is called 1{yields}2 cloning of a measurement. We perform the optimization for both learning and cloning for arbitrary dimension d of the Hilbert space. For 1{yields}2 cloning we also propose a simple quantum network that achieves the optimal fidelity. The optimal fidelity for 1{yields}2 learning just slightly outperforms the estimate and prepare strategy in which one first estimates the unknown measurement and depending on the result suitably prepares the duplicate.

  17. A Clone of Your Own.

    Science.gov (United States)

    Bilodeau, Kirsten

    1997-01-01

    Describes an activity used at the Washington Park Arboretum that helps students understand cloning through plant propagation. Students also learn how to make a pot from recycled newspapers and how to make soil that is appropriate for the plants. (DDR)

  18. Animal Cloning and Food Safety

    Science.gov (United States)

    ... Products For Consumers Home For Consumers Consumer Updates Animal Cloning and Food Safety Share Tweet Linkedin Pin ... safe to eat as food from conventionally bred animals. This conclusion stems from an extensive study of ...

  19. Science Innovation

    Science.gov (United States)

    EPA provides innovative research activities that help transform the protection of human health and the environment with high-risk, high-reward Pathfinder Innovation Projects, the P3 student competition, and low-cost air monitoring.

  20. Technology Innovation

    Science.gov (United States)

    EPA produces innovative technologies and facilitates their creation in line with the Agency mission to create products such as the stormwater calculator, remote sensing, innovation clusters, and low-cost air sensors.

  1. Cloning of low dose radiation induced gene RIG1 by RACE based on non-cloned cDNA library

    International Nuclear Information System (INIS)

    Luo Ying; Sui Jianli; Tie Yi; Zhang Yuanping; Zhou Pingkun; Sun Zhixian

    2001-01-01

    Objective: To obtain full-length cDNA of radiation induced new gene RIG1 based on its EST fragment. Methods: Based on non-cloned cDNA library, enhanced nested RACE PCR and biotin-avidin labelled probe for magnetic bead purification was used to obtain full-length cDNA of RIG1. Results: About 1 kb of 3' end of RIG1 gene was successfully cloned by this set of methods and cloning of RIG1 5' end is proceeding well. Conclusion: The result is consistent with the design of experiment. This set of protocol is useful for cloning of full-length gene based on EST fragment

  2. Caracterização dos pirênios e métodos para acelerar a germinação de sementes de muruci do clone Açu Pyrene characterization and methods to acelerate germination of muruci seeds of the clone Açu

    Directory of Open Access Journals (Sweden)

    José Edmar Urano de Carvalho

    2008-09-01

    ., clone Açu, an accession of the Tropical Fruit Tree Germplasm Collection of Embrapa Eastern Amazonian were characterized and different methods to overcome the seed dormancy were evaluated. Pyrene color, shape, weight, length, diameter, number of seeds per pyrenes, and thickness of the external and internal walls of the endocarp were characterized; seed color, weight, length, diameter and thickness were characterized. The treatments to overcome seed dormancy were: : a control; b soaking in water for 24 hours; c soaking in gibberellicc acid (500 mg.L-1 for 24 hours; d soaking in water for 24 hours followed by compression fracturing of the endocarp; e soaking in gibberellic acid (500 mg.L-1 for 24 hours and fracturing of the endocarp. The pyrenes of muruci "Açu" are ovate, with a reticulate surface design, creamy brown in color, weight 0.62±0.09 g and are 1.10±0.07 cm long by 1.02±0.09 cm in diameter. The pyrenes generally contain two seeds (minimum zero and maximum three, located in the loculus whose external walls are thicker than the internal walls. The endocarp is water permeable and the seeds absorb water rapidly. The seeds represent 10.12±2.87% of the pyrene weight. The best treatments to overcome dormancy were soaking in giberellic acid or water followed by fracturing the endocarp. This combination of results suggests that germination of muruci "Açu" seeds is regulated by exogenous and endogenous dormancy mechanisms. The first is caused by the endocarp, which offers considerable resistance to the growth of the embryo, and the second is the seed physiological dormancy.

  3. Political innovations

    DEFF Research Database (Denmark)

    Sørensen, Eva

    2017-01-01

    are mainly interested in assessing and promoting innovations in public service delivery, but have paid little or no attention to the need for innovations in polity, politics and policy. This article develops a research agenda for studying innovations in political institutions, in the political process...... and in policy outputs. It proposes a number of research themes related to political innovations that call for scholarly attention, and identifies push and pull factors influencing the likelihood that these themes will be addressed in future research....

  4. Islamic perspectives on human cloning.

    Science.gov (United States)

    Sadeghi, Mahmoud

    2007-01-01

    The present paper seeks to assess various views from Islamic jurists relating to human cloning, which is one of the controversial topics in the recent past. Taking Islamic jurisprudence principles, such as the rule of necessity for self preservation and respect for human beings, the rule of la darar wa la dirar ('the necessity to refrain from causing harm to oneself and others') and the rule of usr wa haraj, one may indicate that if human cloning could not be prohibited, as such, it could still be opposed because it gives way to various harmful consequences, which include family disorder, chaos in the clone's family relationships, physical and mental diseases for clones and suffering of egg donors and surrogate mothers. However with due attention to the fact that the reasons behind the prohibition of abortion only restrict the destruction of human embryos in their post-implantation stages, human cloning for biomedical research and exploitation of stem cells from cloned embryos at the blastocyst stage for therapeutic purposes would be acceptable.

  5. Artificial cloning of domestic animals.

    Science.gov (United States)

    Keefer, Carol L

    2015-07-21

    Domestic animals can be cloned using techniques such as embryo splitting and nuclear transfer to produce genetically identical individuals. Although embryo splitting is limited to the production of only a few identical individuals, nuclear transfer of donor nuclei into recipient oocytes, whose own nuclear DNA has been removed, can result in large numbers of identical individuals. Moreover, clones can be produced using donor cells from sterile animals, such as steers and geldings, and, unlike their genetic source, these clones are fertile. In reality, due to low efficiencies and the high costs of cloning domestic species, only a limited number of identical individuals are generally produced, and these clones are primarily used as breed stock. In addition to providing a means of rescuing and propagating valuable genetics, somatic cell nuclear transfer (SCNT) research has contributed knowledge that has led to the direct reprogramming of cells (e.g., to induce pluripotent stem cells) and a better understanding of epigenetic regulation during embryonic development. In this review, I provide a broad overview of the historical development of cloning in domestic animals, of its application to the propagation of livestock and transgenic animal production, and of its scientific promise for advancing basic research.

  6. Quantum cloning machines for equatorial qubits

    International Nuclear Information System (INIS)

    Fan Heng; Matsumoto, Keiji; Wang Xiangbin; Wadati, Miki

    2002-01-01

    Quantum cloning machines for equatorial qubits are studied. For the case of a one to two phase-covariant quantum cloning machine, we present the networks consisting of quantum gates to realize the quantum cloning transformations. The copied equatorial qubits are shown to be separable by using Peres-Horodecki criterion. The optimal one to M phase-covariant quantum cloning transformations are given

  7. Structured Review of Code Clone Literature

    NARCIS (Netherlands)

    Hordijk, W.T.B.; Ponisio, Laura; Wieringa, Roelf J.

    2008-01-01

    This report presents the results of a structured review of code clone literature. The aim of the review is to assemble a conceptual model of clone-related concepts which helps us to reason about clones. This conceptual model unifies clone concepts from a wide range of literature, so that findings

  8. Innovation hubs

    DEFF Research Database (Denmark)

    O´Hara, J.; Hansen, Poul H. Kyvsgård; Turner, N.

    2008-01-01

    "Whilst ‘incremental innovation' is an imperative for the short-to-medium term success of a company, there is also a need for companies to engage in innovation activity that goes beyond the incremental in order to guarantee long-term success. However, such ‘radical innovation' (RI) poses new chal...

  9. Brugerdreven innovation

    DEFF Research Database (Denmark)

    Helms, Niels Henrik; Larsen, Lasse Juel

    2007-01-01

    På et generelt plan er vores afsæt et opgør med traditionelle modeller for kreativitet og innovation. Artiklen mener, at brugerdreven innovation ikke bare en god idé, men en beskrivelse af, hvordan innovation finder sted. Vores afsæt er interaktionsdesign, hvor vi bygger på den antagelse...

  10. KEYBOARD MONITORING BASED UPON THE IMMUNOLOGIC CLONING

    Directory of Open Access Journals (Sweden)

    Yu. A. Bryukhomitsky

    2016-12-01

    Full Text Available The Biometric Keyboard Monitoring System is represented. It’s intended for permanent textindependent control and analysis of automated information data systems users’ keyboard script. It’s suggested a keyboard monitoring method, which is combined the idea and advantages of threaded method of keyboard parameters representation and immunological approach to its realization, based upon the detectors cloning model. Suggested method potentially possesses a pinpoint accuracy, higher convergence rate of classification problems solving, ability to learn on only “own” class exemplars.

  11. Methylotroph cloning vehicle

    Science.gov (United States)

    Hanson, R.S.; Allen, L.N.

    1989-04-25

    A cloning vehicle comprising: a replication determinant effective for replicating the vehicle in a non-C[sub 1]-utilizing host and in a C[sub 1]-utilizing host; DNA effective to allow the vehicle to be mobilized from the non-C[sub 1]-utilizing host to the C[sub 1]-utilizing host; DNA providing resistance to two antibiotics to which the wild-type C[sub 1]-utilizing host is susceptible, each of the antibiotic resistance markers having a recognition site for a restriction endonuclease; a cos site; and a means for preventing replication in the C[sub 1]-utilizing host. The vehicle is used for complementation mapping as follows. DNA comprising a gene from the C[sub 1]-utilizing organism is inserted at the restriction nuclease recognition site, inactivating the antibiotic resistance marker at that site. The vehicle can then be used to form a cosmid structure to infect the non-C[sub 1]-utilizing (e.g., E. coli) host, and then conjugated with a selected C[sub 1]-utilizing mutant. Resistance to the other antibiotic by the mutant is a marker of the conjugation. Other phenotypical changes in the mutant, e.g., loss of an auxotrophic trait, is attributed to the C[sub 1] gene. The vector is also used to inactivate genes whose protein products catalyze side reactions that divert compounds from a biosynthetic pathway to a desired product, thereby producing an organism that makes the desired product in higher yields. 3 figs.

  12. Silver-staining mRNA differential display method and cloning of tumor related genes in HepG2 cell line%银染mRNA差异显示法克隆肝癌相关基因

    Institute of Scientific and Technical Information of China (English)

    王新; 黄裕新; 闻勤生; 王庆莉

    2001-01-01

    AIM To develop mRNA differential display polymerize chain reaction (DD-PCR) method with silver staining and to clone the liver cancer related genes in HepG2 cell line. METHODS Total RNA was extracted from the HepG2 cells and L02 cells. Their first strains of cDNA were produced by reverse transcription (RT). The cDNA were amplified by PCR using an anchored primer 5 dT11G combined with eight arbitrary primers respectively. The products of PCR were analyzed on a denaturing 60 g*L-1 polyacrylamide gel. The differential DNA bands on gel were seen by silver-staining.RESULTS Several liver cancer related genes were isolated and identified by the sensitive silver-staining mRNA differential display developed. CONCLUSION The established mRNA differential display method with silver staining is a simple, efficient means which has higher value for screening and cloning differentially expressed genes.%目的建立银染mRNA差异显示方法,筛选并克隆肝癌相关基因. 方法以建系的人肝癌细胞HepG2和正常的肝细胞L02的总RNA为模板,用5-dT11G锚定引物和8条随机引物(AP1~AP8)组合进行RT-PCR扩增,PCR产物经60 g*L-1尿素变性聚丙烯酰胺凝胶电泳分离后,凝胶银盐染色显示差异的DNA片段. 结果建立了快速敏感的银染mRNA差异显示方法,分离并克隆了一些肝癌相关基因. 结论建立的银染mRNA差异显示法简单、有效,在差异表达基因的筛选中有较高的实用价值.

  13. Local cloning of two product states

    International Nuclear Information System (INIS)

    Ji Zhengfeng; Feng Yuan; Ying Mingsheng

    2005-01-01

    Local quantum operations and classical communication (LOCC) put considerable constraints on many quantum information processing tasks such as cloning and discrimination. Surprisingly, however, discrimination of any two pure states survives such constraints in some sense. We show that cloning is not that lucky; namely, probabilistic LOCC cloning of two product states is strictly less efficient than global cloning. We prove our result by giving explicitly the efficiency formula of local cloning of any two product states

  14. Framing Innovation

    DEFF Research Database (Denmark)

    Haase, Louise Møller; Laursen, Linda Nhu

    2017-01-01

    Designing a remarkable product innovation is a difficult challenge, which businesses today continuously are striving to tackle. This challenge is particularly present in the early phase of innovation, where the main product concept and frames of the innovation is determined. As a main challenge...... in the early phase is the reasoning process; innovation team are faced with open- ended ill-defines problems, where they need to make decisions about an unknown future having only incomplete, ambiguous and contradicting insights available. We study the reasoning of experts, how they frame to make sense of all...... the insights and create a basis for decision making in relation to a new project. Based on case studies of five innovative products from various industries, we suggest a Product Reasoning Model for understanding reasoning and envisioning of new product innovations in the early phases of innovation....

  15. Local cloning of entangled states

    International Nuclear Information System (INIS)

    Gheorghiu, Vlad; Yu Li; Cohen, Scott M.

    2010-01-01

    We investigate the conditions under which a set S of pure bipartite quantum states on a DxD system can be locally cloned deterministically by separable operations, when at least one of the states is full Schmidt rank. We allow for the possibility of cloning using a resource state that is less than maximally entangled. Our results include that: (i) all states in S must be full Schmidt rank and equally entangled under the G-concurrence measure, and (ii) the set S can be extended to a larger clonable set generated by a finite group G of order |G|=N, the number of states in the larger set. It is then shown that any local cloning apparatus is capable of cloning a number of states that divides D exactly. We provide a complete solution for two central problems in local cloning, giving necessary and sufficient conditions for (i) when a set of maximally entangled states can be locally cloned, valid for all D; and (ii) local cloning of entangled qubit states with nonvanishing entanglement. In both of these cases, we show that a maximally entangled resource is necessary and sufficient, and the states must be related to each other by local unitary 'shift' operations. These shifts are determined by the group structure, so need not be simple cyclic permutations. Assuming this shifted form and partially entangled states, then in D=3 we show that a maximally entangled resource is again necessary and sufficient, while for higher-dimensional systems, we find that the resource state must be strictly more entangled than the states in S. All of our necessary conditions for separable operations are also necessary conditions for local operations and classical communication (LOCC), since the latter is a proper subset of the former. In fact, all our results hold for LOCC, as our sufficient conditions are demonstrated for LOCC, directly.

  16. An Innovative Method for Evaluating Strategic Goals in a Public Agency: Conservation Leadership in the U.S. Forest Service

    Science.gov (United States)

    David N. Bengston; David P. Fan

    1999-01-01

    This article presents an innovative methodology for evaluating strategic planning goals in a public agency. Computer-coded content analysis was used to evaluate attitudes expressed in about 28,000 on-line news media stories about the U.S. Department of Agriculture Forest Service and its strategic goal of conservation leadership. Three dimensions of conservation...

  17. Organizational Agility and Complex Enterprise System Innovations: A Mixed Methods Study of the Effects of Enterprise Systems on Organizational Agility

    Science.gov (United States)

    Kharabe, Amol T.

    2012-01-01

    Over the last two decades, firms have operated in "increasingly" accelerated "high-velocity" dynamic markets, which require them to become "agile." During the same time frame, firms have increasingly deployed complex enterprise systems--large-scale packaged software "innovations" that integrate and automate…

  18. Situation in Engineering Universities of Applied Science in Oulu and Eindhoven to Teach Methods to Achieve Innovations in Businesses

    NARCIS (Netherlands)

    Dr.Ir. Hay Geraedts

    2014-01-01

    The importance of teaching engineering students innovation development is commonly clearly understood. It is essential to achieve products which are attractive and profitable in the market. To achieve this, an institute of engineering education has to provide students with needed knowledge, skills

  19. Contribution to pressure vessels design of innovative methods and comparative application with standardized rules on a realistic structure – Part I

    International Nuclear Information System (INIS)

    Rohart, Philippe; Panier, Stéphane; Hariri, Saïd; Simonet, Yves; Afzali, Mansour

    2015-01-01

    Design of pressure vessels, which are subjected to various natures of loading, must prevent damage mechanisms occurrence. For a load applied or maintained with a given intensity, primary failure modes can appear, such as gross plastic deformation, plastic instability or buckling. For design-by-analysis, the reference methodology is based on an elastic stress calculation. During the last decade, studies have shown that this ingenious procedure could provide conservative design limits. They can become actually overly conservative in a context of increasing complexity of geometry and loading modelling. In parallel, technological and theoretical developments enabled limit analysis to be considered as an interesting design methodology. This is suggested in standards and codes (EN 13445, CODAP, Boiler and Pressure Vessels Code) since the early 2000"'"s. In this first of two companion papers, a set of standardized and innovative procedures is introduced. These approaches rely on various concepts, such as elasticity, incremental elastoplasticity, or elastic compensation (Modified Elastic Compensation Method, Linear Matching Method). Each methodology is presented on theoretical aspects, eventually adapted so as to take into account safety margins. They are then applied on a model inspired from a real industrial reactor, using Abaqus. Results are compared to reference data from codes, in terms of accuracy and computing time. A final assessment underlines practical benefits that could be expected. - Highlights: • A review of pressure vessels design methods against gross plastic deformation is made. • Innovative methodologies are introduced in order to overcome practical limits of classical methods. • Comparative tests are performed on one Benchmark with both classical and innovative procedures. • Results show the ability of innovative methodologies to improve the ratio ‘accuracy’ – ‘computationnal time’.

  20. Advancing Clouds Lifecycle Representation in Numerical Models Using Innovative Analysis Methods that Bridge ARM Observations and Models Over a Breadth of Scales

    Energy Technology Data Exchange (ETDEWEB)

    Kollias, Pavlos [McGill Univ., Montreal, QC (Canada

    2016-09-06

    This the final report for the DE-SC0007096 - Advancing Clouds Lifecycle Representation in Numerical Models Using Innovative Analysis Methods that Bridge ARM Observations and Models Over a Breadth of Scales - PI: Pavlos Kollias. The final report outline the main findings of the research conducted using the aforementioned award in the area of cloud research from the cloud scale (10-100 m) to the mesoscale (20-50 km).

  1. Marshall Barber and the century of microinjection: from cloning of bacteria to cloning of everything.

    Science.gov (United States)

    Korzh, Vladimir; Strähle, Uwe

    2002-08-01

    A hundred years ago, Dr. Marshall A. Barber proposed a new technique - the microinjection technique. He developed this method initially to clone bacteria and to confirm the germ theory of Koch and Pasteur. Later on, he refined his approach and was able to manipulate nuclei in protozoa and to implant bacteria into plant cells. Continuous improvement and adaptation of this method to new applications dramatically changed experimental embryology and cytology and led to the formation of several new scientific disciplines including animal cloning as one of its latest applications. Interestingly, microinjection originated as a method at the crossroad of bacteriology and plant biology, demonstrating once again the unforeseen impact that basic research in an unrelated field can have on the development of entirely different disciplines.

  2. Genotype-environment interaction and stability in ten-year height growth of Norway spruce Clones (Picea abies Karst.).

    Science.gov (United States)

    J.B. St. Clair; J. Kleinschmit

    1986-01-01

    Norway spruce cuttings of 40 clones were tested on seven contrasting sites in northern Germany. Analysis of variance for ten-year height growth indicate a highly significant clone x site interaction. This interaction may be reduced by selection of stable clones. Several measures of stability were calculated and discussed. Characterization of sites by the method of...

  3. [Cloning and law in Hungary].

    Science.gov (United States)

    Julesz, Máté

    2015-03-01

    Reproductive human cloning is prohibited in Hungary, as in many other countries. Therapeutic human cloning is not prohibited, just like in many other countries. Stem cell therapy is also allowed. Article III, paragraph (3) of the Hungarian basic law (constitution) strictly forbids total human cloning. Article 1 of the Additional Protocol to the Oviedo Convention, on the Prohibition of Cloning Human Beings (1998) stipulates that any intervention seeking to create a human being genetically identical to another human being, whether living or dead, is prohibited. In Hungary, according to Article 174 of the Criminal Code, total human cloning constitutes a crime. Article 180, paragraph (3) of the Hungarian Act on Health declares that embryos shall not be brought about for research purposes; research shall be conducted only on embryos brought about for reproductive purposes when this is authorized by the persons entitled to decide upon its disposal, or when the embryo is damaged. Article 180, paragraph (5) of the Hungarian Act on Health stipulates that multiple individuals who genetically conform to one another shall not be brought about. According to Article 181, paragraph (1) of the Hungarian Act on Health, an embryo used for research shall be kept alive for not longer than 14 days, not counting the time it was frozen for storage and the time period of research.

  4. Garlic breeding system innovations

    NARCIS (Netherlands)

    Zheng, S.J.; Kamenetsky, R.; Féréol, L.; Barandiaran, X.; Rabinowitch, H.D.; Chovelon, V.; Kik, C.

    2007-01-01

    This review outlines innovative methods for garlic breeding improvement and discusses the techniques used to increase variation like mutagenesis and in vitro techniques, as well as the current developments in florogenesis, sexual hybridization, genetic transformation and mass propagation. Sexual

  5. Creating a testing field where delta technology and water innovations are tested and demonstrated with the help of citizen science methods

    Science.gov (United States)

    de Vries, Sandra; Rutten, Martine; de Vries, Liselotte; Anema, Kim; Klop, Tanja; Kaspersma, Judith

    2017-04-01

    In highly populated deltas, much work is to be done. Complex problems ask for new and knowledge driven solutions. Innovations in delta technology and water can bring relief to managing the water rich urban areas. Testing fields form a fundamental part of the knowledge valorisation for such innovations. In such testing fields, product development by start-ups is coupled with researchers, thus supplying new scientific insights. With the help of tests, demonstrations and large-scale applications by the end-users, these innovations find their way to the daily practices of delta management. More and more cities embrace the concept of Smart Cities to tackle the ongoing complexity of urban problems and to manage the city's assets - such as its water supply networks and other water management infrastructure. Through the use of new technologies and innovative systems, data are collected from and with citizens and devices - then processed and analysed. The information and knowledge gathered are keys to enabling a better quality of life. By testing water innovations together with citizens in order to find solutions for water management problems, not only highly spatial amounts of data are provided by and/or about these innovations, they are also improved and demonstrated to the public. A consortium consisting of a water authority, a science centre, a valorisation program and two universities have joined forces to create a testing field for delta technology and water innovations using citizen science methods. In this testing field, the use of citizen science for water technologies is researched and validated by facilitating pilot projects. In these projects, researchers, start-ups and citizens work together to find the answer to present-day water management problems. The above mentioned testing field tests the use of crowd-sourcing data as for example hydrological model inputs, or to validate remote sensing applications, or improve water management decisions. Currently the

  6. The topsy-turvy cloning law.

    Science.gov (United States)

    Brassington, Iain; Oultram, Stuart

    2011-03-01

    In debates about human cloning, a distinction is frequently drawn between therapeutic and reproductive uses of the technology. Naturally enough, this distinction influences the way that the law is framed. The general consensus is that therapeutic cloning is less morally problematic than reproductive cloning--one can hold this position while holding that both are morally unacceptable--and the law frequently leaves the way open for some cloning for the sake of research into new therapeutic techniques while banning it for reproductive purposes. We claim that the position adopted by the law has things the wrong way around: if we accept a moral distinction between therapeutic and reproductive cloning, there are actually more reasons to be morally worried about therapeutic cloning than about reproductive cloning. If cloning is the proper object of legal scrutiny, then, we ought to make sure that we are scrutinising the right kind of clone.

  7. Generating West Nile Virus from an Infectious Clone.

    Science.gov (United States)

    Vandergaast, Rianna; Fredericksen, Brenda L

    2016-01-01

    WNV infectious clones are valuable tools for elucidating WNV biology. Nevertheless, relatively few infectious WNV clones have been generated because their construction is hampered by the instability of flaviviral genomes. More recently, advances in cloning techniques as well as the development of several two-plasmid WNV infectious clone systems have facilitated the generation of WNV infectious clones. Here we described a protocol for recovering WNV from a two-plasmid system. In this approach, large quantities of these constructs are digested with restriction enzymes to produce complementary restriction sites at the 3' end of the upstream fragment and the 5' end of the downstream fragment. These fragments are then annealed to produce linear template for in vitro transcription to synthesize infectious RNA. The resulting RNA is transfected into cells and after several days WNV is recovered in the culture supernatant. This method can be used to generate virus from infectious clones encoding high- and low-pathogenicity strains of WNV, as well as chimeric virues.

  8. Framing Innovation

    DEFF Research Database (Denmark)

    Haase, Louise Møller; Laursen, Linda Nhu

    2017-01-01

    Designing a remarkable product innovation is a difficult challenge, which businesses today continuously are striving to tackle. This challenge is particularly present in the early phase of innovation, where the main product concept and frames of the innovation is determined. As a main challenge...... in the early phase is the reasoning process; innovation team are faced with open-ended ill-defines problems, where they need to make decisions about an unknown future having only incomplete, ambiguous and contradicting insights available. We study the reasoning of experts, how they frame to make sense of all...... the insights and create a basis for decision making in relation to a new project. Based on case studies of five innovative products from various industries, we suggest a Product Reasoning Model for understanding reasoning and envisioning of new product innovations in the early phases...

  9. Online Innovation

    DEFF Research Database (Denmark)

    Vujovic, Sladjana; Ulhøi, John Parm

    2008-01-01

      Purpose - The aim of this paper is to investigate the role of online networking during the innovation process, including its role(s) in communication, cooperation and coordination. The paper neither implicitly assumes that online computer-based networking is a prerequisite for the innovation...... process nor denies the possibility that innovation can emerge and successfully survive without it. It merely presupposes that, in cases of innovation where information and communication technologies play a substantial role, non-proprietarity may offer an interesting alternative to innovations based...... on proprietary knowledge. Design/methodology/approach - The paper borrows from the theory of communities-of-practice, which takes into account social relations, contacts, and the transfer and incorporation of knowledge. Open source innovation is not the exclusive preserve of computer nerds, but also has...

  10. Sensitive innovation

    DEFF Research Database (Denmark)

    Søndergaard, Katia Dupret

    Present paper discusses sources of innovation as heterogenic and at times intangible processes. Arguing for heterogeneity and intangibility as sources of innovation originates from a theoretical reading in STS and ANT studies (e.g. Callon 1986, Latour 1996, Mol 2002, Pols 2005) and from field work...... in the area of mental health (Dupret Søndergaard 2009, 2010). The concept of sensitive innovation is developed to capture and conceptualise exactly those heterogenic and intangible processes. Sensitive innovation is therefore primarily a way to understand innovative sources that can be......, but are not necessarily, recognized and acknowledged as such in the outer organisational culture or by management. The added value that qualifies these processes to be defined as “innovative” are thus argued for along different lines than in more traditional innovation studies (e.g. studies that build on the classic...

  11. Public perceptions of animal cloning

    DEFF Research Database (Denmark)

    Jelsøe, Erling; Vincentsen, Ulla; Andersen, Ida-Elisabeth

    What was from the outset meant to be a survey testing predefined categories of ethical positions related to new biotechnologies with animal cloning as an example was subsequently developed into a process of broader involvement of groups of citizens in the issue. The survey was conducted at meetings...... in four different cities in Denmark. The participants were introduced to animal cloning and after that they filled out the questionnaire. Finally, the issue was discussed in focus groups. The process as a whole was run in a dialogue oriented way. Through the information they received in combination...... with reflecting on the survey questions the participants were well prepared for discussions in the focus groups. This approach made it possible, on the one hand to get a measure of the citizen's perceptions of the ethical aspects of animal cloning, but also to go deeper into their own thoughts of the issue...

  12. A novel nucleo-cytoplasmic hybrid clone formed via androgenesis in polyploid gibel carp

    Directory of Open Access Journals (Sweden)

    Zhou Li

    2011-03-01

    Full Text Available Abstract Background Unisexual vertebrates have been demonstrated to reproduce by gynogenesis, hybridogenesis, parthenogenesis, or kleptogenesis, however, it is uncertain how the reproduction mode contributes to the clonal diversity. Recently, polyploid gibel carp has been revealed to possess coexisting dual modes of unisexual gynogenesis and sexual reproduction and to have numerous various clones. Using sexual reproduction mating between clone D female and clone A male and subsequent 7 generation multiplying of unisexual gynogenesis, we have created a novel clone strain with more than several hundred millions of individuals. Here, we attempt to identify genetic background of the novel clone and to explore the significant implication for clonal diversity contribution. Methods Several nuclear genome markers and one cytoplasmic marker, the mitochondrial genome sequence, were used to identify the genetic organization of the randomly sampled individuals from different generations of the novel clone. Results Chromosome number, Cot-1 repetitive DNA banded karyotype, microsatellite patterns, AFLP profiles and transferrin alleles uniformly indicated that nuclear genome of the novel clone is identical to that of clone A, and significantly different from that of clone D. However, the cytoplasmic marker, its complete mtDNA genome sequence, is same to that of clone D, and different from that of clone A. Conclusions The present data indicate that the novel clone is a nucleo-cytoplasmic hybrid between the known clones A and D, because it originates from the offspring of gonochoristic sexual reproduction mating between clone D female and clone A male, and contains an entire nuclear genome from the paternal clone A and a mtDNA genome (cytoplasm from the maternal clone D. It is suggested to arise via androgenesis by a mechanism of ploidy doubling of clone A sperm in clone D ooplasm through inhibiting the first mitotic division. Significantly, the selected nucleo

  13. A novel nucleo-cytoplasmic hybrid clone formed via androgenesis in polyploid gibel carp

    Science.gov (United States)

    2011-01-01

    Background Unisexual vertebrates have been demonstrated to reproduce by gynogenesis, hybridogenesis, parthenogenesis, or kleptogenesis, however, it is uncertain how the reproduction mode contributes to the clonal diversity. Recently, polyploid gibel carp has been revealed to possess coexisting dual modes of unisexual gynogenesis and sexual reproduction and to have numerous various clones. Using sexual reproduction mating between clone D female and clone A male and subsequent 7 generation multiplying of unisexual gynogenesis, we have created a novel clone strain with more than several hundred millions of individuals. Here, we attempt to identify genetic background of the novel clone and to explore the significant implication for clonal diversity contribution. Methods Several nuclear genome markers and one cytoplasmic marker, the mitochondrial genome sequence, were used to identify the genetic organization of the randomly sampled individuals from different generations of the novel clone. Results Chromosome number, Cot-1 repetitive DNA banded karyotype, microsatellite patterns, AFLP profiles and transferrin alleles uniformly indicated that nuclear genome of the novel clone is identical to that of clone A, and significantly different from that of clone D. However, the cytoplasmic marker, its complete mtDNA genome sequence, is same to that of clone D, and different from that of clone A. Conclusions The present data indicate that the novel clone is a nucleo-cytoplasmic hybrid between the known clones A and D, because it originates from the offspring of gonochoristic sexual reproduction mating between clone D female and clone A male, and contains an entire nuclear genome from the paternal clone A and a mtDNA genome (cytoplasm) from the maternal clone D. It is suggested to arise via androgenesis by a mechanism of ploidy doubling of clone A sperm in clone D ooplasm through inhibiting the first mitotic division. Significantly, the selected nucleo-cytoplasmic hybrid female

  14. Value innovations in accordance to Apple

    OpenAIRE

    Mertl, Jiří

    2011-01-01

    This bachelor's thesis is aimed at comparison of value management way of innovations and innovations according to Apple. At the first part there are analyzed theoretical, methodical and practical aspects of both of ways. Key theme for value innovation is value for customer, which is analyzed from both points of view value management and Apple. Except of product innovations there are mentioned for example marketing innovations or process innovations. In last part of the thesis there are demons...

  15. Financing Innovation

    OpenAIRE

    William R. Kerr; Ramana Nanda

    2014-01-01

    We review the recent literature on the financing of innovation, inclusive of large companies and new startups. This research strand has been very active over the past five years, generating important new findings, questioning some long-held beliefs, and creating its own puzzles. Our review outlines the growing body of work that documents a role for debt financing related to innovation. We highlight the new literature on learning and experimentation across multi-stage innovation projects and h...

  16. Cloning of Plasmodium falciparum by single-cell sorting.

    Science.gov (United States)

    Miao, Jun; Li, Xiaolian; Cui, Liwang

    2010-10-01

    Malaria parasite cloning is traditionally carried out mainly by using the limiting dilution method, which is laborious, imprecise, and unable to distinguish multiply-infected RBCs. In this study, we used a parasite engineered to express green fluorescent protein (GFP) to evaluate a single-cell sorting method for rapidly cloning Plasmodium falciparum. By dividing a two-dimensional scattergram from a cell sorter into 17 gates, we determined the parameters for isolating singly-infected erythrocytes and sorted them into individual cultures. Pre-gating of the engineered parasites for GFP allowed the isolation of almost 100% GFP-positive clones. Compared with the limiting dilution method, the number of parasite clones obtained by single-cell sorting was much higher. Molecular analyses showed that parasite isolates obtained by single-cell sorting were highly homogenous. This highly efficient single-cell sorting method should prove very useful for cloning both P. falciparum laboratory populations from genetic manipulation experiments and clinical samples. Copyright 2010 Elsevier Inc. All rights reserved.

  17. Cloning of Plasmodium falciparum by single-cell sorting

    Science.gov (United States)

    Miao, Jun; Li, Xiaolian; Cui, Liwang

    2010-01-01

    Malaria parasite cloning is traditionally carried out mainly by using the limiting dilution method, which is laborious, imprecise, and unable to distinguish multiply-infected RBCs. In this study, we used a parasite engineered to express green fluorescent protein (GFP) to evaluate a single-cell sorting method for rapidly cloning Plasmodium falciparum. By dividing a two dimensional scattergram from a cell sorter into 17 gates, we determined the parameters for isolating singly-infected erythrocytes and sorted them into individual cultures. Pre-gating of the engineered parasites for GFP allowed the isolation of almost 100% GFP-positive clones. Compared with the limiting dilution method, the number of parasite clones obtained by single-cell sorting was much higher. Molecular analyses showed that parasite isolates obtained by single-cell sorting were highly homogenous. This highly efficient single-cell sorting method should prove very useful for cloning both P. falciparum laboratory populations from genetic manipulation experiments and clinical samples. PMID:20435038

  18. Supplier Innovation

    DEFF Research Database (Denmark)

    Søberg, Peder Veng; Notman, Dorian Mark; Wæhrens, Brian Vejrum

    2017-01-01

    The prevailing research in supplier innovation has been conceptual or based on small data sets. The analysis of a large data set gives a clearer perspective on the actual type and degree of involvement of suppliers in the customer’s innovation process. The supplier perspective of the research...... counterbalances the customer bias that exists in the prevailing literature in this area. Similarly, the issue of the benefit of supplier involvement in the innovation/NPD process can be better understood. Manufacturing suppliers benefit financially from their involvement in the innovation/NPD process of their key...

  19. Responsive Innovation

    DEFF Research Database (Denmark)

    Pedersen, Carsten

    Although the importance of stakeholder networks has been recognized in recent years, a non-teleological model that incorporates their collective sensing into innovation processes has so far not been developed. Hence, this paper argues that traditional linear and sequential innovation models...... are insufficient in hypercompetitive environments. Instead, it is proposed that companies should ground their innovation processes in the collective sensing of frontline-employees and customers that operate around the organizational periphery. This frames the concept of responsive innovation, where key...... stakeholders engaged in the organization’s ongoing business activities collectively identify issues that central managers subsequently can resolve....

  20. Innovation @ NASA

    Science.gov (United States)

    Roman, Juan A.

    2014-01-01

    This presentation provides an overview of the activities National Aeronautics and Space Administration (NASA) is doing to encourage innovation across the agency. All information provided is available publicly.

  1. Analytical methods and simulation models to assess innovative operational measures and technologies for rail port terminals: the case of Valencia Principe Felipe terminal

    Energy Technology Data Exchange (ETDEWEB)

    Ricci, S.

    2016-07-01

    The topic of freight transport by rail is a complex theme and, in recent years, a main issue of European policy. The legislation evolution and the White Paper 2011 have demonstrated the European intention to re-launch this sector. The challenge is to promote the intermodal transport system to the detriment of road freight transport. In this context intermodal freight terminals, play a primary role for the supply chain, they are the connection point between the various transport nodes and the nodal points where the freight are handled, stored and transferred between different modes to final customer. To achieve the purpose, it is strengthen the improvement of existing intermodal freight terminals and the development of innovative intermodal freight terminals towards higher performance (ERRAC, 2012). Many terminal performances improvements have been proposed and sometime experimented. They are normally basing on combinations of operational measures and innovative technologies (e.g. automatic horizontal and parallel storage and handling, automated gate and sensors for tracking systems data exchange) tested in various terminals, with often-contradictory results. The research work described in this paper (developed within the Capacity4Rail EU project) focusses on the assessment of effects that these innovations can have in the intermodal freight terminals combined in various alternative consistent effective scenarios. The methodological framework setup to assess these innovations is basing on a combination of analytical methods based on sequential algorithms and discrete events simulation models. The output of this assessment method are key performance indicators (KPIs) selected according to terminals typologies and related to different aspects (e.g. management, operation and organization). The present paper illustrates the application of the methodological framework, tuned on the operation of various intermodal terminals, for the validation on today operation and the

  2. Quantum cloning machines and the applications

    Energy Technology Data Exchange (ETDEWEB)

    Fan, Heng, E-mail: hfan@iphy.ac.cn [Beijing National Laboratory for Condensed Matter Physics, Institute of Physics, Chinese Academy of Sciences, Beijing 100190 (China); Collaborative Innovation Center of Quantum Matter, Beijing 100190 (China); Wang, Yi-Nan; Jing, Li [School of Physics, Peking University, Beijing 100871 (China); Yue, Jie-Dong [Beijing National Laboratory for Condensed Matter Physics, Institute of Physics, Chinese Academy of Sciences, Beijing 100190 (China); Shi, Han-Duo; Zhang, Yong-Liang; Mu, Liang-Zhu [School of Physics, Peking University, Beijing 100871 (China)

    2014-11-20

    No-cloning theorem is fundamental for quantum mechanics and for quantum information science that states an unknown quantum state cannot be cloned perfectly. However, we can try to clone a quantum state approximately with the optimal fidelity, or instead, we can try to clone it perfectly with the largest probability. Thus various quantum cloning machines have been designed for different quantum information protocols. Specifically, quantum cloning machines can be designed to analyze the security of quantum key distribution protocols such as BB84 protocol, six-state protocol, B92 protocol and their generalizations. Some well-known quantum cloning machines include universal quantum cloning machine, phase-covariant cloning machine, the asymmetric quantum cloning machine and the probabilistic quantum cloning machine. In the past years, much progress has been made in studying quantum cloning machines and their applications and implementations, both theoretically and experimentally. In this review, we will give a complete description of those important developments about quantum cloning and some related topics. On the other hand, this review is self-consistent, and in particular, we try to present some detailed formulations so that further study can be taken based on those results.

  3. Quantum cloning machines and the applications

    International Nuclear Information System (INIS)

    Fan, Heng; Wang, Yi-Nan; Jing, Li; Yue, Jie-Dong; Shi, Han-Duo; Zhang, Yong-Liang; Mu, Liang-Zhu

    2014-01-01

    No-cloning theorem is fundamental for quantum mechanics and for quantum information science that states an unknown quantum state cannot be cloned perfectly. However, we can try to clone a quantum state approximately with the optimal fidelity, or instead, we can try to clone it perfectly with the largest probability. Thus various quantum cloning machines have been designed for different quantum information protocols. Specifically, quantum cloning machines can be designed to analyze the security of quantum key distribution protocols such as BB84 protocol, six-state protocol, B92 protocol and their generalizations. Some well-known quantum cloning machines include universal quantum cloning machine, phase-covariant cloning machine, the asymmetric quantum cloning machine and the probabilistic quantum cloning machine. In the past years, much progress has been made in studying quantum cloning machines and their applications and implementations, both theoretically and experimentally. In this review, we will give a complete description of those important developments about quantum cloning and some related topics. On the other hand, this review is self-consistent, and in particular, we try to present some detailed formulations so that further study can be taken based on those results

  4. Innovation process

    DEFF Research Database (Denmark)

    Kolodovski, A.

    2006-01-01

    Purpose of this report: This report was prepared for RISO team involved in design of the innovation system Report provides innovation methodology to establish common understanding of the process concepts and related terminology The report does not includeRISO- or Denmark-specific cultural, econom...

  5. Open innovation

    DEFF Research Database (Denmark)

    Bogers, Marcel; Chesbrough, Henry; Moedas, Carlos

    2018-01-01

    Open innovation is now a widely used concept in academia, business, and policy making. This article describes the state of open innovation at the intersection of research, practice, and policy. It discusses some key trends (e.g., digital transformation), challenges (e.g., uncertainty...

  6. Managing Innovation.

    Science.gov (United States)

    White, Ronald V.

    The management of innovation in instruction in English as a second language can benefit from the experience and techniques of management in the world of commerce as well as from theories and procedures in education. A systematic approach to the management of innovation emphasizes the importance of clearly defined objectives, evaluation that…

  7. EasyClone-MarkerFree

    DEFF Research Database (Denmark)

    Fabre, Mathew Malcolm Jessop; Jakociunas, Tadas; Stovicek, Vratislav

    2016-01-01

    Clone-MarkerFree. The integration of linearized expression cassettes into defined genomic loci is facilitated by CRISPR/Cas9. Cas9 is recruited to the chromosomal location by specific guide RNAs (gRNAs) expressed from a set of gRNA helper vectors. Using our genome engineering vector suite, single and triple insertions are obtained...

  8. Clone Poems and the Microcomputer.

    Science.gov (United States)

    Irizarry, Estelle

    1989-01-01

    Describes how students can use the computer to study and create clone poems (altering original Spanish-language poems by substituting words and expressions), and how students can gain a deeper appreciation of the original poem's poetic structure and semantics. (CB)

  9. MAPPING INNOVATION

    DEFF Research Database (Denmark)

    Thuesen, Christian Langhoff; Koch, Christian

    2011-01-01

    By adopting a theoretical framework from strategic niche management research (SNM) this paper presents an analysis of the innovation system of the Danish Construction industry. Theories within SNM look upon innovation in a sector as a socio-technical phenomenon and identifies three levels of socio......-technical interaction within which sectorial innovation can be explained. The analysis shows a multifaceted landscape of innovation around an existing regime, built in the existing ways of working and developing over generations. The regime is challenged from various niches and the sociotechnical landscape through...... trends as globalization. Three niches (Lean Construction, BIM and System Deliveries) are subject to a detailed analysis showing partly incompatible rationales and various degrees of innovation potential. The paper further discusses how existing policymaking operates in a number of tensions one being...

  10. Accidental Innovation

    DEFF Research Database (Denmark)

    Austin, Robert D.; Devin, Lee; Sullivan, Erin E.

    2012-01-01

    Historical accounts of human achievement suggest that accidents can play an important role in innovation. In this paper, we seek to contribute to an understanding of how digital systems might support valuable unpredictability in innovation processes by examining how innovators who obtain value from...... they incorporate accidents into their deliberate processes and arranged surroundings. By comparing makers working in varied conditions, we identify specific factors (e.g., technologies, characteristics of technologies) that appear to support accidental innovation. We show that makers in certain specified...... conditions not only remain open to accident but also intentionally design their processes and surroundings to invite and exploit valuable accidents. Based on these findings, we offer advice for the design of digital systems to support innovation processes that can access valuable unpredictability....

  11. Collaborative innovation

    DEFF Research Database (Denmark)

    Torfing, Jacob; Sørensen, Eva; Hartley, Jean

    2013-01-01

    , which emphasizes market competition; the neo-Weberian state, which emphasizes organizational entrepreneurship; and collaborative governance, which emphasizes multiactor engagement across organizations in the private, public, and nonprofit sectors. The authors conclude that the choice of strategies......-driven private sector is more innovative than the public sector by showing that both sectors have a number of drivers of as well as barriers to innovation, some of which are similar, while others are sector specific. The article then systematically analyzes three strategies for innovation: New Public Management......There are growing pressures for the public sector to be more innovative but considerable disagreement about how to achieve it. This article uses institutional and organizational analysis to compare three major public innovation strategies. The article confronts the myth that the market...

  12. Probabilistic cloning and deleting of quantum states

    International Nuclear Information System (INIS)

    Feng Yuan; Zhang Shengyu; Ying Mingsheng

    2002-01-01

    We construct a probabilistic cloning and deleting machine which, taking several copies of an input quantum state, can output a linear superposition of multiple cloning and deleting states. Since the machine can perform cloning and deleting in a single unitary evolution, the probabilistic cloning and other cloning machines proposed in the previous literature can be thought of as special cases of our machine. A sufficient and necessary condition for successful cloning and deleting is presented, and it requires that the copies of an arbitrarily presumed number of the input states are linearly independent. This simply generalizes some results for cloning. We also derive an upper bound for the success probability of the cloning and deleting machine

  13. Human reproductive cloning: a conflict of liberties.

    Science.gov (United States)

    Havstad, Joyce C

    2010-02-01

    Proponents of human reproductive cloning do not dispute that cloning may lead to violations of clones' right to self-determination, or that these violations could cause psychological harms. But they proceed with their endorsement of human reproductive cloning by dismissing these psychological harms, mainly in two ways. The first tactic is to point out that to commit the genetic fallacy is indeed a mistake; the second is to invoke Parfit's non-identity problem. The argument of this paper is that neither approach succeeds in removing our moral responsibility to consider and to prevent psychological harms to cloned individuals. In fact, the same commitment to personal liberty that generates the right to reproduce by means of cloning also creates the need to limit that right appropriately. Discussion of human reproductive cloning ought to involve a careful and balanced consideration of both the relevant aspects of personal liberty - the parents' right to reproductive freedom and the cloned child's right to self-determination.

  14. Evaluation of Quantitative and Qualitative Traits of 18 Potato Clones

    Directory of Open Access Journals (Sweden)

    A. R Bolandi

    2016-10-01

    to those of check. Based on results of this experiment, the clones 397003-7, 396151-27 and 397045-100 could be selected for Ardebil region. The objective of this research was to evaluate the quantitative and qualitative traits of cultivars and advanced potato clones in spring cultivation (Jolge-e-Rokh region. Materials and Methods In this research, 18 potato clones for the quantitative and qualitative traits were compared with three check cultivars including: Agria (suitable for French-fries, Marfona (suitable for boiled eating and Lady Rosetta (suitable for chips in Jolge-Rokh Agriculture Research Station, the location 35'¸ 50° north latitude and 59° east longitude and 1721 m above sea level, in crop year 2011. Experimental design was Randomized Complete Block Design (RCBD with three replications. Treatment consisted of 18 advanced potato clones: 396151-8, 397045-4, 397045-10, TP12-13, TP21-29, TP12-8, 397007-16, 397007-17, 396140-6, 397009-8, 397015-14, 397003-7, 396151-27, 397045-100, 397097-9, 396151-20, 397045-7 and 69 indigenous with three control cultivars (Agria, Marfona and Lady Rosetta. Tubers were planted on two rows with 6 meters length. Distance between row and plants on the row were 75 and 25 cm, respectively. Area of each plot was 9 square meter. The evaluated characters were total yield, marketable yield, eye number, dry matter percentage, tuber number per plant, tuber weight per plant and mean of tuber weight. In order to measure total yield, after maturity, and remove the aerial organs, all of the tubers were harvested and the fresh weight was obtained. After removal of the tumor in bad shape, with soft rot and smaller than 30 mm that cannot be sold as part of the marketable yield, rest of them were used to measure marketable yield. Tuber dry matter percentage (TDM% was determined from the relationship between fresh and dry weights of sub-sample of 8-10 thinly sliced tubers dried for 48 h at 80° C. Data were analyzed using SAS 9.1 software. The

  15. Quantum dot-based molecular imaging of cancer cell growth using a clone formation assay.

    Science.gov (United States)

    Geng, Xia-Fei; Fang, Min; Liu, Shao-Ping; Li, Yan

    2016-10-01

    This aim of the present study was to investigate clonal growth behavior and analyze the proliferation characteristics of cancer cells. The MCF‑7 human breast cancer cell line, SW480 human colon cancer cell line and SGC7901 human gastric cancer cell line were selected to investigate the morphology of cell clones. Quantum dot‑based molecular targeted imaging techniques (which stained pan‑cytokeratin in the cytoplasm green and Ki67 in the cell nucleus yellow or red) were used to investigate the clone formation rate, cell morphology, discrete tendency, and Ki67 expression and distribution in clones. From the cell clone formation assay, the MCF‑7, SW480 and SGC7901 cells were observed to form clones on days 6, 8 and 12 of cell culture, respectively. These three types of cells had heterogeneous morphology, large nuclear:cytoplasmic ratios, and conspicuous pathological mitotic features. The cells at the clone periphery formed multiple pseudopodium. In certain clones, cancer cells at the borderline were separated from the central cell clusters or presented a discrete tendency. With quantum dot‑based molecular targeted imaging techniques, cells with strong Ki67 expression were predominantly shown to be distributed at the clone periphery, or concentrated on one side of the clones. In conclusion, cancer cell clones showed asymmetric growth behavior, and Ki67 was widely expressed in clones of these three cell lines, with strong expression around the clones, or aggregated at one side. Cell clone formation assay based on quantum dots molecular imaging offered a novel method to study the proliferative features of cancer cells, thus providing a further insight into tumor biology.

  16. Phase-covariant quantum cloning of qudits

    International Nuclear Information System (INIS)

    Fan Heng; Imai, Hiroshi; Matsumoto, Keiji; Wang, Xiang-Bin

    2003-01-01

    We study the phase-covariant quantum cloning machine for qudits, i.e., the input states in a d-level quantum system have complex coefficients with arbitrary phase but constant module. A cloning unitary transformation is proposed. After optimizing the fidelity between input state and single qudit reduced density operator of output state, we obtain the optimal fidelity for 1 to 2 phase-covariant quantum cloning of qudits and the corresponding cloning transformation

  17. Probabilistic cloning of three symmetric states

    International Nuclear Information System (INIS)

    Jimenez, O.; Bergou, J.; Delgado, A.

    2010-01-01

    We study the probabilistic cloning of three symmetric states. These states are defined by a single complex quantity, the inner product among them. We show that three different probabilistic cloning machines are necessary to optimally clone all possible families of three symmetric states. We also show that the optimal cloning probability of generating M copies out of one original can be cast as the quotient between the success probability of unambiguously discriminating one and M copies of symmetric states.

  18. Interactive Methods for Teaching Action Potentials, an Example of Teaching Innovation from Neuroscience Postdoctoral Fellows in the Fellowships in Research and Science Teaching (FIRST) Program

    Science.gov (United States)

    Keen-Rhinehart, E.; Eisen, A.; Eaton, D.; McCormack, K.

    2009-01-01

    Acquiring a faculty position in academia is extremely competitive and now typically requires more than just solid research skills and knowledge of one’s field. Recruiting institutions currently desire new faculty that can teach effectively, but few postdoctoral positions provide any training in teaching methods. Fellowships in Research and Science Teaching (FIRST) is a successful postdoctoral training program funded by the National Institutes of Health (NIH) providing training in both research and teaching methodology. The FIRST program provides fellows with outstanding interdisciplinary biomedical research training in fields such as neuroscience. The postdoctoral research experience is integrated with a teaching program which includes a How to Teach course, instruction in classroom technology and course development and mentored teaching. During their mentored teaching experiences, fellows are encouraged to explore innovative teaching methodologies and to perform science teaching research to improve classroom learning. FIRST fellows teaching neuroscience to undergraduates have observed that many of these students have difficulty with the topic of neuroscience. Therefore, we investigated the effects of interactive teaching methods for this topic. We tested two interactive teaching methodologies to determine if they would improve learning and retention of this information when compared with standard lectures. The interactive methods for teaching action potentials increased understanding and retention. Therefore, FIRST provides excellent teaching training, partly by enhancing the ability of fellows to integrate innovative teaching methods into their instruction. This training in turn provides fellows that matriculate from this program more of the characteristics that hiring institutions desire in their new faculty. PMID:23493377

  19. A single-copy galK promoter cloning vector suitable for cloning strong promoters

    DEFF Research Database (Denmark)

    Dandanell, Gert; Court, Donald L.; Hammer, Karin

    1986-01-01

    We report the construction of lambda galK promoter cloning vectors for cloning and characterization of strong promoters. This phage, which contains a unique HindIII cloning site, was applied to the cloning and analysis of transcription initiations of the regulatory region of the deo-operon of...

  20. Preliminary assessment of the grading of Eucalyptus clones using carbon isotope discrimination

    International Nuclear Information System (INIS)

    Bond, W.J.; Stock, W.D.

    1990-01-01

    Stable carbon isotopes were analysed in leaf material of nine Eucalyptus clones grown in field trials in the eastern Transvaal. Carbon isotope ratios, measured as d 13 C, differed within tree canopies, between replicate trees and between clones. Values from both north and south canopy positions were correlated with tree height after 13 months. Unexplained variation in the correlation may be interpreted, theoretically, as an indication that some clones use less water for the same level of productivity. With further testing, the method may have promise for early screening of clones in genotype/environment interaction trials and in selecting water-efficient trees. 3 figs., 3 tabs., 7 refs

  1. ReMixT: clone-specific genomic structure estimation in cancer.

    Science.gov (United States)

    McPherson, Andrew W; Roth, Andrew; Ha, Gavin; Chauve, Cedric; Steif, Adi; de Souza, Camila P E; Eirew, Peter; Bouchard-Côté, Alexandre; Aparicio, Sam; Sahinalp, S Cenk; Shah, Sohrab P

    2017-07-27

    Somatic evolution of malignant cells produces tumors composed of multiple clonal populations, distinguished in part by rearrangements and copy number changes affecting chromosomal segments. Whole genome sequencing mixes the signals of sampled populations, diluting the signals of clone-specific aberrations, and complicating estimation of clone-specific genotypes. We introduce ReMixT, a method to unmix tumor and contaminating normal signals and jointly predict mixture proportions, clone-specific segment copy number, and clone specificity of breakpoints. ReMixT is free, open-source software and is available at http://bitbucket.org/dranew/remixt .

  2. Innovation and Entrepreneurship in Education

    DEFF Research Database (Denmark)

    , and mindsets. Focusing on ubiquitous learning and transcending the borders of formal, informal, and non-formal learning, the book presents theoretical models and case studies of initiatives, practices, and projects on how innovative pedagogies and ICT could support the development of innovation......-of-the-art in innovation and entrepreneurship in education as seen through the latest academic studies and business currents.......This book explores the concepts of innovation and entrepreneurship through multiple lenses in the context of education. Mixing equal parts of theory and practice, this volume takes a closer look on how innovation and entrepreneurship are approached around the globe as disciplines, methods...

  3. Nuclear innovation in Saskatchewan: innovation

    International Nuclear Information System (INIS)

    Alexander, N.

    2015-01-01

    This paper describes nuclear innovation in Saskatchewan. The first stage is the Canadian Institute for Science and Innovation Policy (CSIP) and how you have a successful discussion about a technically complex issue, understand what information people need in order to have an informed discussion, understand how to convey that information to those people in a constructive way.

  4. Efficient preparation of shuffled DNA libraries through recombination (Gateway) cloning.

    Science.gov (United States)

    Lehtonen, Soili I; Taskinen, Barbara; Ojala, Elina; Kukkurainen, Sampo; Rahikainen, Rolle; Riihimäki, Tiina A; Laitinen, Olli H; Kulomaa, Markku S; Hytönen, Vesa P

    2015-01-01

    Efficient and robust subcloning is essential for the construction of high-diversity DNA libraries in the field of directed evolution. We have developed a more efficient method for the subcloning of DNA-shuffled libraries by employing recombination cloning (Gateway). The Gateway cloning procedure was performed directly after the gene reassembly reaction, without additional purification and amplification steps, thus simplifying the conventional DNA shuffling protocols. Recombination-based cloning, directly from the heterologous reassembly reaction, conserved the high quality of the library and reduced the time required for the library construction. The described method is generally compatible for the construction of DNA-shuffled gene libraries. © The Author 2014. Published by Oxford University Press. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com.

  5. Bengal Bay clone ST772-MRSA-V outbreak: conserved clone causes investigation challenges.

    Science.gov (United States)

    Blomfeldt, A; Larssen, K W; Moghen, A; Haugum, K; Steen, T W; Jørgensen, S B; Aamot, H V

    2017-03-01

    The Bengal Bay clone, ST772-MRSA-V, associated with multi-drug resistance, Panton-Valentine leukocidin (PVL) and skin and soft tissue infections, is emerging worldwide. In Norway, a country with low prevalence of meticillin-resistant Staphylococcus aureus (MRSA), increased occurrence of ST772-MRSA-V has also caused hospital outbreaks. The conserved nature of this clone challenged the outbreak investigations. To evaluate the usefulness of S. aureus protein A (spa) typing, multiple-locus variable number tandem repeat fingerprinting/analysis (MLVF/MLVA) and pulsed-field gel electrophoresis (PFGE) when investigating outbreaks with a conserved MRSA clone. A panel of 25 MRSA isolates collected in 2004-2014, consisting of six hospital outbreak isolates and 19 sporadic isolates, were analysed using spa typing, polymerase chain reaction detection of genes encoding PVL, MLVF/MLVA and PFGE. All isolates were ST772-MRSA-V-t657 and resistant to erythromycin, gentamicin and norfloxacin, and 88% were PVL positive. PFGE could not discriminate between the isolates (≥85% similarity). MLVF resolved five types [Simpson's index of diversity (SID)=0.56], MLVA resolved six types (SID=0.66), and both methods separated the hospital isolates into two defined outbreaks. MLVF/MLVA could not discriminate all epidemiologically unlinked cases and identical genotypes originated from a timespan of 10 years. MLVA was regarded as most suitable due to its higher discriminatory power and ability to provide unambiguous profiles. However, the Bengal Bay clone may require higher resolution methods for exact demarcation of outbreaks due to low diversity among isolates. Copyright © 2016 The Healthcare Infection Society. Published by Elsevier Ltd. All rights reserved.

  6. Participatory Innovation

    DEFF Research Database (Denmark)

    Buur, Jacob; Matthews, Ben

    In this paper we discuss the potential for Participatory Design (PD) to make a fundamental contribution to the business-oriented field of user-driven innovation, taking note of where we find PD can best benefit from interaction with this other field. We examine some of the challenges that must...... be addressed if PD is to contribute to innovation processes in companies. We conclude by presenting a research agenda comprising of six promising topics to shape a new discipline of Participatory Innovation....

  7. Design & Innovation

    DEFF Research Database (Denmark)

    Boelskifte, Per; Jørgensen, Ulrik

    2005-01-01

    The new design and innovation programme at DTU is challenging some of the standard concepts dominating most engineering educations. The programme, its background, context and basic educational ideas are presented and discussed in this paper. To build competences that match the need for innovative...... and design oriented engineers in industry and society has turned out to challenge a number of the standard – and often taken for granted – concepts in engineering education still dominated world wide by rather strict norms and concepts of learning that do not challenge the students creativity and innovative...

  8. Measuring Consumer Innovativeness: Identifying Innovators among Consumers of Modern Technologies

    Directory of Open Access Journals (Sweden)

    Jana Filová

    2015-12-01

    Full Text Available The methods currently used in innovation marketing research are focused on the late phases of the innovation process and are usually methodologically complex. This limits their practical impact. The presented work aims to create a simple self-report scale applicable in the initial and late phases of the innovation process, highly modular and suitable for a wide range of research. The main battery of questions was inspired by the adopter categorization by Rogers. The questions determine both (1 general characteristics of innovation adopters and (2 their relationship to a specific innovation. The scale was tested during robust longitudinal online research, thematically focused on users of modern technologies. A representative sample of 4,000 Internet users in the Czech Republic took part in the survey from 2013 to 2015. The result is a new self-report scale measuring consumer innovativeness applicable for prototyping, strategic decisions and effective communication of innovations to consumers.

  9. No-cloning theorem on quantum logics

    International Nuclear Information System (INIS)

    Miyadera, Takayuki; Imai, Hideki

    2009-01-01

    This paper discusses the no-cloning theorem in a logicoalgebraic approach. In this approach, an orthoalgebra is considered as a general structure for propositions in a physical theory. We proved that an orthoalgebra admits cloning operation if and only if it is a Boolean algebra. That is, only classical theory admits the cloning of states. If unsharp propositions are to be included in the theory, then a notion of effect algebra is considered. We proved that an atomic Archimedean effect algebra admitting cloning operation is a Boolean algebra. This paper also presents a partial result, indicating a relation between the cloning on effect algebras and hidden variables.

  10. Therapeutic and reproductive cloning: a critique.

    Science.gov (United States)

    Bowring, Finn

    2004-01-01

    This article is a critical examination of the science and ethics of human cloning. It summarises the key scientific milestones in the development of nuclear transplantation, explains the importance of cloning to research into the medical potential of embryonic stem cells, and discusses the well-worn distinction between 'therapeutic' and 'reproductive' cloning. Suggesting that this distinction will be impossible to police, it goes on to consider the ethics of full human cloning. It is concluded that it represents an unacceptable form of parental despotism, and that the genetic engineering and cloning of future human beings will fracture the foundations of modern humanism.

  11. Innovation and Safety. A prestudy; Innovation och saekerhet. En foerstudie

    Energy Technology Data Exchange (ETDEWEB)

    Rollenhagen, Carl; Hansson, Sven Ove; Hortberg, Johan; Jakobsson, Fredrik; Zhau, Victoria Jing; Mojeri, Sara

    2010-04-15

    The project summarized in this report was initiated to explore relations between innovation and safety. The first two sections of the report discuss some previously conducted research and give a general background to the subject. It is concluded that safety research and innovation research, by and large, has developed as separate academic disciplines. The concepts of 'innovative safety culture' and 'safe innovation cultures' are suggested as two concepts that can be used to integrate research: innovative safety cultures depart from safety culture research but attempts to introduce an innovative dimension with the aim to create adaptive and innovative safety cultures that efficiently can handle risks arising from existing innovations. Safe innovation cultures have focus on innovation itself, but with the ambition to introduce concepts and methods from safety research in the innovative processes. Three subprojects conducted in the context of the present research are summarized. The first project examines how an existing organization (e.g. SKB - Swedish Nuclear Fuel and Waste Management) attempts to integrate both innovative activities and operative activities in the same organisation. Interviews with key personnel explored different views about how innovative and safety work coexists in the organisation. The second project focuses on how major retrofit projects of a nuclear power plant is managed in parallel to operative activities (e.g. operating the plant on an everyday basis). By means of an innovative technique (e.g. system groups) seminars were held to suggest improvements in the technical change process. The third project conducted a risk analysis of a major organisational change (e.g. control centres for energy distribution). Experiences from the three projects are finally discussed in terms of similarities and differences associated with the cultures for innovation and safety. Suggestions for further research are made

  12. MAPPING INNOVATION

    DEFF Research Database (Denmark)

    Thuesen, Christian Langhoff; Koch, Christian

    2011-01-01

    By adopting a theoretical framework from strategic niche management research (SNM) this paper presents an analysis of the innovation system of the Danish Construction industry. The analysis shows a multifaceted landscape of innovation around an existing regime, built around existing ways of working...... and developed over generations. The regime is challenged from various niches and the socio-technical landscape through trends as globalization. Three niches (Lean Construction, BIM and System Deliveries) are subject to a detailed analysis showing partly incompatible rationales and various degrees of innovation...... potential. The paper further discusses how existing policymaking operates in a number of tensions one being between government and governance. Based on the concepts from SNM the paper introduces an innovation map in order to support the development of meta-governance policymaking. By mapping some...

  13. Innovation Awards

    Science.gov (United States)

    EPA promotes environmental stewardship by recognizing innovators in schools, communities and businesses in categories such as environmental education, green chemistry, smart growth, green power, and reducing air pollution and climate change impacts.

  14. Clone DB: an integrated NCBI resource for clone-associated data

    Science.gov (United States)

    Schneider, Valerie A.; Chen, Hsiu-Chuan; Clausen, Cliff; Meric, Peter A.; Zhou, Zhigang; Bouk, Nathan; Husain, Nora; Maglott, Donna R.; Church, Deanna M.

    2013-01-01

    The National Center for Biotechnology Information (NCBI) Clone DB (http://www.ncbi.nlm.nih.gov/clone/) is an integrated resource providing information about and facilitating access to clones, which serve as valuable research reagents in many fields, including genome sequencing and variation analysis. Clone DB represents an expansion and replacement of the former NCBI Clone Registry and has records for genomic and cell-based libraries and clones representing more than 100 different eukaryotic taxa. Records provide details of library construction, associated sequences, map positions and information about resource distribution. Clone DB is indexed in the NCBI Entrez system and can be queried by fields that include organism, clone name, gene name and sequence identifier. Whenever possible, genomic clones are mapped to reference assemblies and their map positions provided in clone records. Clones mapping to specific genomic regions can also be searched for using the NCBI Clone Finder tool, which accepts queries based on sequence coordinates or features such as gene or transcript names. Clone DB makes reports of library, clone and placement data on its FTP site available for download. With Clone DB, users now have available to them a centralized resource that provides them with the tools they will need to make use of these important research reagents. PMID:23193260

  15. Production of cloned mice and ES cells from adult somatic cells by nuclear transfer: how to improve cloning efficiency?

    Science.gov (United States)

    Wakayama, Teruhiko

    2007-02-01

    Although it has now been 10 years since the first cloned mammals were generated from somatic cells using nuclear transfer (NT), most cloned embryos usually undergo developmental arrest prior to or soon after implantation, and the success rate for producing live offspring by cloning remains below 5%. The low success rate is believed to be associated with epigenetic errors, including abnormal DNA hypermethylation, but the mechanism of "reprogramming" is unclear. We have been able to develop a stable NT method in the mouse in which donor nuclei are directly injected into the oocyte using a piezo-actuated micromanipulator. Especially in the mouse, only a few laboratories can make clones from adult somatic cells, and cloned mice are never successfully produced from most mouse strains. However, this technique promises to be an important tool for future research in basic biology. For example, NT can be used to generate embryonic stem (NT-ES) cell lines from a patient's own somatic cells. We have shown that NT-ES cells are equivalent to ES cells derived from fertilized embryos and that they can be generated relatively easily from a variety of mouse genotypes and cell types of both sexes, even though it may be more difficult to generate clones directly. In general, NT-ES cell techniques are expected to be applied to regenerative medicine; however, this technique can also be applied to the preservation of genetic resources of mouse strain instead of embryos, oocytes and spermatozoa. This review describes how to improve cloning efficiency and NT-ES cell establishment and further applications.

  16. Characterization of chlorinated solvent contamination in limestone using innovative FLUTe® technologies in combination with other methods in a line of evidence approach

    DEFF Research Database (Denmark)

    Broholm, Mette Martina; Janniche, Gry Sander; Mosthaf, Klaus

    2016-01-01

    Characterization of dense non-aqueous phase liquid (DNAPL) source zones in limestone aquifers/bedrock is essential to develop accurate site-specific conceptual models and perform risk assessment. Here innovative field methods were combined to improve determination of source zone architecture......, hydrogeology and contaminant distribution. The FACT™ is a new technology and it was applied and tested at a contaminated site with a limestone aquifer, together with a number of existing methods including wire-line coring with core subsampling, FLUTe® transmissivity profiling and multilevel water sampling...... groundwater sampling (under two flow conditions) and FACT™ sampling and analysis combined with FLUTe® transmissivity profiling and modeling were used to provide a line of evidence for the presence of DNAPL, dissolved and sorbed phase contamination in the limestone fractures and matrix. The combined methods...

  17. Leadership Innovations

    Science.gov (United States)

    1993-04-01

    leadership " and " leadership in innovation." 3 THEORY , AS AN INSTRUMENT OF ANALYSIS FOR INNOVATION IN LEADERSHIP There are many...attributes of a leader.𔃺 Attempts to define leadership usually contrast it with "followship", management ," or sometimes - with ’beadship."’" These...plausible theory . - . . . -. - -- Nevertheless, some authors consider, and I agree, that there is no true leadership theory because existing

  18. Quantitative discrimination of Aggregatibacter actinomycetemcomitans highly leukotoxic JP2 clone from non-JP2 clones in diagnosis of aggressive periodontitis

    Directory of Open Access Journals (Sweden)

    Yoshida Akihiro

    2012-10-01

    Full Text Available Abstract Background Aggregatibacter actinomycetemcomitans is the etiological agent of periodontitis, and there is a strong association between clone JP2 and aggressive periodontitis in adolescents of African descent. The JP2 clone has an approximately 530-bp deletion (∆530 in the promoter region of the lkt/ltx gene, which encodes leukotoxin, and this clone has high leukotoxic activity. Therefore, this clone is very important in aggressive periodontitis. To diagnose this disease, culture methods and conventional PCR techniques are used. However, quantitative detection based on qPCR for the JP2 clone has not been developed due to genetic difficulties. In this study, we developed a qPCR-based quantification method specific to the JP2 clone. Methods Based on our analysis of the DNA sequence of the lkt/ltx gene and its flanking region, we designed a reverse primer specific for the ∆530 deletion border sequence and developed a JP2-specific PCR-based quantification method using this primer. We also analyzed the DNA sequence of the ∆530 locus and found it to be highly conserved (97–100% among 17 non-JP2 strains. Using the ∆530 locus, we designed a qPCR primer–probe set specific to non-JP2 clones. Next, we determined the numbers of JP2 and non-JP2 clone cells in the periodontal pockets of patients with aggressive periodontitis. Results The JP2-specific primers specifically amplified the genomic DNA of the A. actinomycetemcomitans JP2 clone and did not react with other bacterial DNA, whereas the non-JP2 specific primers reacted only with A. actinomycetemcomitans non-JP2 clones. Samples from the 88 periodontal sites in the 11 patients with aggressive periodontitis were analyzed. The bacterial cell numbers in 88 periodontal sites ranged from 0 to 4.8 × 108 (mean 1.28 × 107 for JP2 clones and from 0 to 1.6 × 106 for non-JP2 clones (mean 1.84 × 105. There were significant differences in the JP2 cell number between a clinical attachment level

  19. Solid-phase cloning for high-throughput assembly of single and multiple DNA parts

    DEFF Research Database (Denmark)

    Lundqvist, Magnus; Edfors, Fredrik; Sivertsson, Åsa

    2015-01-01

    We describe solid-phase cloning (SPC) for high-throughput assembly of expression plasmids. Our method allows PCR products to be put directly into a liquid handler for capture and purification using paramagnetic streptavidin beads and conversion into constructs by subsequent cloning reactions. We ...

  20. Molecular cloning of growth hormone encoding cDNA of Indian

    Indian Academy of Sciences (India)

    A modified rapid amplification of cDNA ends (RACE) strategy has been developed for cloning highly conserved cDNA sequences. Using this modified method, the growth hormone (GH) encoding cDNA sequences of Labeo rohita, Cirrhina mrigala and Catla catla have been cloned, characterized and overexpressed in ...

  1. Implementing phase-covariant cloning in circuit quantum electrodynamics

    Energy Technology Data Exchange (ETDEWEB)

    Zhu, Meng-Zheng [School of Physics and Material Science, Anhui University, Hefei 230039 (China); School of Physics and Electronic Information, Huaibei Normal University, Huaibei 235000 (China); Ye, Liu, E-mail: yeliu@ahu.edu.cn [School of Physics and Material Science, Anhui University, Hefei 230039 (China)

    2016-10-15

    An efficient scheme is proposed to implement phase-covariant quantum cloning by using a superconducting transmon qubit coupled to a microwave cavity resonator in the strong dispersive limit of circuit quantum electrodynamics (QED). By solving the master equation numerically, we plot the Wigner function and Poisson distribution of the cavity mode after each operation in the cloning transformation sequence according to two logic circuits proposed. The visualizations of the quasi-probability distribution in phase-space for the cavity mode and the occupation probability distribution in the Fock basis enable us to penetrate the evolution process of cavity mode during the phase-covariant cloning (PCC) transformation. With the help of numerical simulation method, we find out that the present cloning machine is not the isotropic model because its output fidelity depends on the polar angle and the azimuthal angle of the initial input state on the Bloch sphere. The fidelity for the actual output clone of the present scheme is slightly smaller than one in the theoretical case. The simulation results are consistent with the theoretical ones. This further corroborates our scheme based on circuit QED can implement efficiently PCC transformation.

  2. Cloning transformations in spin networks without external control

    International Nuclear Information System (INIS)

    De Chiara, Gabriele; Fazio, Rosario; Montangero, Simone; Macchiavello, Chiara; Palma, G. Massimo

    2005-01-01

    In this paper we present an approach to quantum cloning with unmodulated spin networks. The cloner is realized by a proper design of the network and a choice of the coupling between the qubits. We show that in the case of phase covariant cloner the XY coupling gives the best results. In the 1→2 cloning we find that the value for the fidelity of the optimal cloner is achieved, and values comparable to the optimal ones in the general N→M case can be attained. If a suitable set of network symmetries are satisfied, the output fidelity of the clones does not depend on the specific choice of the graph. We show that spin network cloning is robust against the presence of static imperfections. Moreover, in the presence of noise, it outperforms the conventional approach. In this case the fidelity exceeds the corresponding value obtained by quantum gates even for a very small amount of noise. Furthermore, we show how to use this method to clone qutrits and qudits. By means of the Heisenberg coupling it is also possible to implement the universal cloner although in this case the fidelity is 10% off that of the optimal cloner

  3. Cloning Expeditions: Risky but Rewarding

    Science.gov (United States)

    2013-01-01

    In the 1980s, a good part of my laboratory was using the then-new recombinant DNA techniques to clone and characterize many important cell surface membrane proteins: GLUT1 (the red cell glucose transporter) and then GLUT2 and GLUT4, the red cell anion exchange protein (Band 3), asialoglycoprotein receptor subunits, sucrase-isomaltase, the erythropoietin receptor, and two of the subunits of the transforming growth factor β (TGF-β) receptor. These cloned genes opened many new fields of basic research, including membrane insertion and trafficking of transmembrane proteins, signal transduction by many members of the cytokine and TGF-β families of receptors, and the cellular physiology of glucose and anion transport. They also led to many insights into the molecular biology of several cancers, hematopoietic disorders, and diabetes. This work was done by an exceptional group of postdocs and students who took exceptionally large risks in developing and using novel cloning technologies. Unsurprisingly, all have gone on to become leaders in the fields of molecular cell biology and molecular medicine. PMID:24061478

  4. Identification of a new geographically widespread multiresistant Acinetobacter baumannii clone from European hospitals.

    Science.gov (United States)

    van Dessel, Helke; Dijkshoorn, Lenie; van der Reijden, Tanny; Bakker, Nancy; Paauw, Armand; van den Broek, Peterhans; Verhoef, Jan; Brisse, Sylvain

    2004-03-01

    The aim of the study was to investigate the genetic diversity of Acinetobacter baumannii clinical strains that had previously been allocated to three major groups based on automated ribotyping. Forty-seven isolates from European hospitals and one isolate from a South African hospital, geographically representative of the three ribogroups (ribogroups 1, 2 and 3 with 10, 23 and 15 isolates, respectively), were analysed using the highly discriminatory fingerprinting methods AFLP and pulsed-field gel electrophoresis (PFGE). Based on AFLP data, the isolates clustered into three main groups, each corresponding to one ribogroup. Inclusion of reference strains of the previously described clones I and II, responsible for outbreaks in northwestern European hospitals, showed that ribogroups 1 and 2 correspond to clones I and II, respectively, whereas ribogroup 3 apparently represents a new clone. This clone III was found in France, The Netherlands, Italy and Spain. Clones I and II were not limited to northwestern European countries, as they were also recovered from Spain, South Africa, Poland and Italy (clone I) and from Spain, Portugal, South Africa, France, Greece and Turkey (clone II). Combined AFLP and PFGE data showed intraclonal diversity and led to the distinction of 23 different genotypes. Three genotypes, two of them belonging to clone II and one to clone III, were found in different hospitals and may correspond to subsets of isolates with a more recent clonal relationship, which emphasizes the epidemic potential of these organisms.

  5. New ways of organizing innovation work

    DEFF Research Database (Denmark)

    Grex, Sara; Møller, Niels

    2006-01-01

    There is an intensified focus in innovation and companies ability to create innovation. In many companies these activities are organized by projects, but studies show this innovation is stifled in this structure. In this paper we argue that there is a need for developing alternative ways of organ......There is an intensified focus in innovation and companies ability to create innovation. In many companies these activities are organized by projects, but studies show this innovation is stifled in this structure. In this paper we argue that there is a need for developing alternative ways...... of organizing innovative activities in project-based settings. We propose the Contextual Design method as a way of both studying innovation work processes and an approach to redesign the innovation work organization. We find that the method can contribute to a better understanding of the innovation work...

  6. Innovation and Safety. A prestudy

    International Nuclear Information System (INIS)

    Rollenhagen, Carl; Hansson, Sven Ove; Hortberg, Johan; Jakobsson, Fredrik; Zhau, Victoria Jing; Mojeri, Sara

    2010-04-01

    The project summarized in this report was initiated to explore relations between innovation and safety. The first two sections of the report discuss some previously conducted research and give a general background to the subject. It is concluded that safety research and innovation research, by and large, has developed as separate academic disciplines. The concepts of 'innovative safety culture' and 'safe innovation cultures' are suggested as two concepts that can be used to integrate research: innovative safety cultures depart from safety culture research but attempts to introduce an innovative dimension with the aim to create adaptive and innovative safety cultures that efficiently can handle risks arising from existing innovations. Safe innovation cultures have focus on innovation itself, but with the ambition to introduce concepts and methods from safety research in the innovative processes. Three subprojects conducted in the context of the present research are summarized. The first project examines how an existing organization (e.g. SKB - Swedish Nuclear Fuel and Waste Management) attempts to integrate both innovative activities and operative activities in the same organisation. Interviews with key personnel explored different views about how innovative and safety work coexists in the organisation. The second project focuses on how major retrofit projects of a nuclear power plant is managed in parallel to operative activities (e.g. operating the plant on an everyday basis). By means of an innovative technique (e.g. system groups) seminars were held to suggest improvements in the technical change process. The third project conducted a risk analysis of a major organisational change (e.g. control centres for energy distribution). Experiences from the three projects are finally discussed in terms of similarities and differences associated with the cultures for innovation and safety. Suggestions for further research are made

  7. The Formation of Methodical Approach to Estimation of Scientific-Research, Scientific-Technical, and Innovative Activity of Institutions of Higher Education

    Directory of Open Access Journals (Sweden)

    Yermachenko Volodymyr Ye.

    2017-12-01

    Full Text Available The research is aimed at developing a methodical approach to the construction of an innovative system of estimation of research, scientific, technical, and innovative activities of higher education institutions (HEI on the basis of elaborate structured system of indicators. The proposed methodical approach contains five stages, the research discloses the content of each of them. Based on the results of a comparative analysis of international and national systems of HEI-rating, as well as the usage of economic and mathematical instrumentarium, a list of indicators for estimating their scientific activities in terms of the selected factors and directions of estimation was formed and substantiated. An approach to formation of cluster aggregates of HEI by the criterion of their scientific performance has been suggested. Proposals on formation of a HEI-rating within the limits of the allocated homogeneous groups by means of the system of integral indicators were developed. Prospects for further scientific researches in this direction imply formation of directions of development of managerial decisions in accordance with the obtained results of rating, which will be directed on strengthening and search for new ways of development of scientific activity of HEI, increase of its level of competitiveness and image, and also strengthening of its contribution to the solution of actual problems of economy and society.

  8. Innovation through accelerators: A case for open innovation

    Directory of Open Access Journals (Sweden)

    Lerato E. Mohalajeng

    2016-12-01

    Full Text Available Background: Open innovation is becoming a progressive business practice in Southern Africa because it plays a significant role in economic development through promoting the commercialisation of new ideas. The challenge is that while the benefits of open innovation are widely spoken about, not much is understood about the challenges and successes of open innovation accelerators (OIAs in taking ideas to market. Aim: The purpose of this research was to investigate an OIA in South Africa for taking ideas to market. Setting: The Innovation Hub is a science park in Pretoria, South Africa, using open innovation to stimulate entrepreneurship in South Africa. Through The Innovation Hub Open IX, a webbased platform, an opportunity to investigate the bridging from invention to commercialisation is presented. Methods: A qualitative research method using semi-structured, in-depth interviews was applied to collect data. Five key stakeholders of the OIA were interviewed. Results: The findings suggest that stakeholder buy-in is essential for commercialisation through OIAs in South Africa. By involving stakeholders in the initial phases of the open innovation process, the likelihood of a solution being incorporated and fitted into the organisation’s business strategy is increased. Conclusion: The insight gained from this research suggests policymakers, research institutions and commercial businesses ought to explore various innovations across industries relevant to their open innovation proficiencies. This research makes a significant contribution to an indepth understanding of what is needed to bridge the gap from invention to successful commercialisation through open innovation.

  9. Policy Innovation in Innovation Policy

    DEFF Research Database (Denmark)

    Borras, Susana

    During the past two decades Europe has experienced important changes and transformations in the way in which governments approach the issue of science, technology and innovation, and their relation to economic growth and competitiveness. This has to do with the European Union level as well...... as with national and sub-national governments in Europe, all of them introducing interesting novelties in their innovation policy. These changes refer to different aspects of policy, mainly the content of policy initiatives towards science, technology and innovation; the instruments governments are using...... at the EU level, and mentions similar trends taking place at national and sub-national levels. The questions that guide the contents here are essentially three, namely, what are the main traits of innovation policies in Europe since the 1990s and how have the EU and different national governments approached...

  10. Inculcating health awareness in Karachi, Pakistan: How innovative, socially acceptable methods can help combat communicable diseases of poverty

    Directory of Open Access Journals (Sweden)

    Maryam Huda

    2017-06-01

    Full Text Available In the megacity of Karachi, which has a population of more than 24 million, more than 9.2 million people (approximately 40 per cent live in squatter settlements. Communities here are characterised by low socioeconomic settings, crowded living conditions, inadequate water and sanitation facilities, and poor health-related behaviours. Such conditions create an environment favourable to the spread of communicable diseases like tuberculosis (TB, hepatitis and dengue. Since 1985, the Department of Community Health Sciences at the Aga Khan University, Karachi, Pakistan has run the Urban Health Program (UHP, a community-campus partnership operating in these disadvantaged squatter settlements. Recent explosive increases in the spread of dengue, hepatitis and TB, however, necessitated special attention and activities on a scale and pace that was greater than could be accommodated as part of UHP’s core work. Thus, having an already well-established collaborative model with social accountability measures in place, a dedicated mass awareness program was initiated over the course of one year, from mid 2015: the AGAHI project. This article describes AGAHI’s innovative, low-cost, collaborative activities conducted in partnership with two squatter communities, Sultanabad and Rehri Goth, to build health awareness, improved care-seeking and compliance to treatment. Activities ranged from school sessions, role plays and awareness walks to laneway meetings, training of health care workers, door-to-door campaigns and collaboration with local religious leaders, public sector groups and NGOs. Building on the collaborative work of the UHP, in just 12 months AGAHI was able to conduct 80 health awareness sessions with 4000 participants. Moreover, high-risk and vulnerable populations were identified and referred for further treatment. A comparative cross-sectional survey afterwards revealed a significant increase in knowledge among Sultanabad residents as compared to

  11. ELSY neutronic analysis by deterministic and Monte Carlo methods. An innovative concept for the control rod systems

    International Nuclear Information System (INIS)

    Artioli, Carlo; Sarotto, Massimo; Grasso, Giacomo; Krepel, Jiri

    2009-01-01

    This paper deals with the neutronic design of ELSY (the European Lead-cooled SYstem), a 600 MW e Fast Reactor developed within the 6th EURATOM Framework Programme. ELSY aims at being an 'adiabatic' system (as far as possible) in order to fulfill both the requirements of sustainability and proliferation resistance. It represents the European solution for the Lead Fast Reactor (LFR), one of the six candidate typologies proposed by the Generation-IV International Forum (GIF). The analysis of the ELSY reference configuration, with typical pure MOX loading, is here presented. An introductory investigation of the adiabatic and, possibly, the burner options viability is also achieved by providing a rough estimate of the Minor Actinides (MAs) equilibrium concentrations and time constants. One of the main challenge-points in the design of the core, made up of wrapper-less square Fuel Assemblies (FAs) according to the common scheme of PWRs, is the small delta-T between the coolant average outlet temperature (480degC) and the allowable cladding one (550degC): it requires a rather flat radial power distribution, obtained by segmenting the core in three zones with different enrichments. Three different control sets have been introduced in order to achieve the required reliability for reactor shutdown and safety systems: eight traditional concept Control Rod (CR) assemblies together with two independent systems of sparse control 'Finger Absorber' Rods (FARs), small B 4 C rods that can be inserted, in principle, in the center of each FA. One of the two finger absorber systems includes a subset of rods devoted to the regulation of the criticality swing during the cycle: their number can be limited indeed since the small reactivity swing (some hundreds pcm) due to the about unitary breeding ratio. Such an innovative solution can also be positioned in order to maintain an optimal power flattening during the fuel cycle. To verify the feasibility of this solution, a very detailed

  12. THE ADAPTIVE NATURE OF MANAGING SOFTWARE INNOVATION

    OpenAIRE

    Mihai Liviu Despa

    2013-01-01

    The focus of this article is pointed at adaptive management in the context of innovative software projects. Software development is presented through the filter of innovation. The aspects that differentiate software innovation from any other kind of innovation are highlighted. Adaptive management is addressed from a general point of view. The circumstances that require adaptive management are emphasized. Methods of implementing adaptive management in innovation oriented software projects are ...

  13. Cloning and expression of cell wall acid invertase gene fragment ...

    African Journals Online (AJOL)

    A fragment of invertase gene containing catalytic sites of cysteine was cloned from poinsettia (Euphorbia pulcherrima wild.) by using the polymerase chain reaction (PCR) method. The length of the fragment was 521 bp, encoding 173 amino acids and containing a part of open reading frames, but no intron. It had a high ...

  14. Differential responses of two rubber tree clones to chilling stress ...

    African Journals Online (AJOL)

    Chilling stress is one of the most important environmental factors that limit the growth, distribution and yield of rubber tree in China. The effects of chilling stress on the grated plants of two rubber trees clones, GT1 and Wenchang217, were studied by physiological methods in controlled light chamber in order to explore the ...

  15. Molecular cloning and characterization of pathogenesis-related ...

    African Journals Online (AJOL)

    use

    2011-12-21

    Dec 21, 2011 ... Available online at http://www.academicjournals.org/AJB ... November, 2011. We described the cloning and characterization of pathogenesis-related protein 5 gene in maize, named .... in two inbred lines was calculated using the ↵Ct method. .... Of the characterized PRs currently known, PR-1, PR-2,. PR-3 ...

  16. Comparative evaluation of prokaryotic 16S rDNA clone libraries and SSCP in groundwater samples.

    Science.gov (United States)

    Larentis, Michael; Alfreider, Albin

    2011-06-01

    A comparison of ribosomal RNA sequence analysis methods based on clone libraries and single-strand conformational polymorphism technique (SSCP) was performed with groundwater samples obtained between 523-555 meters below surface. The coverage of analyzed clones by phylotype-richness estimates was between 88-100%, confirming that the clone libraries were adequately examined. Analysis of individual bands retrieved from SSCP gels identified 1-6 different taxonomic units per band, suggesting that a single SSCP band does often represent more than one single prokaryotic species. The prokaryotic diversity obtained by both methods showed an overall difference of 42-80%. In comparison to SSCP, clone libraries underestimated the phylogenetic diversity and only 36-66% of the phylotypes observed with SSCP were also detected with the clone libraries. An exception was a sample where the SSCP analysis of Archaea identified only half of the phylotypes retrieved by the clone library. Overall, this study suggests that the clone library and the SSCP approach do not provide an identical picture of the prokaryotic diversity in groundwater samples. The results clearly show that the SSCP method, although this approach is prone to generate methodological artifacts, was able to detect significantly more phylotypes than microbial community analysis based on clone libraries. Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  17. The MEDIA model: An innovative method for digitizing and training community members to facilitate an HIV prevention intervention.

    Science.gov (United States)

    Renfro, Tiffaney; Johnson, Erin; Lambert, Danielle N; Wingood, Gina; DiClemente, Ralph J

    2018-02-17

    As human immunodeficiency virus (HIV) continues to disproportionately affect African American women, practitioners remain committed to developing innovative strategies to reduce HIV prevalence. These strategies include training community organizations, such as churches, and utilizing digital media to make intervention dissemination more sustainable. This article describes one such effort to train lay community members within predominantly Black churches in Atlanta, GA, to implement an HIV prevention intervention. Lay educators were trained by translating a face-to-face Training of Facilitators (TOF) to a digital platform using the MEDIA (Motivate-Engage-Digitize-Implement-Assess) model. Formative evaluations, consultation with experts in the digital platform of choice, and the experience of two P4 for Women Master Trainers informed our translation. The model guided the translation process as our research team worked alongside topical experts and a production company to develop storyboards for core curriculum activities, which were later scripted and filmed with mock participants. A user guide, toolkit, and program website were also developed as supplemental materials to accompany the video training. Lessons learned from this study indicate future attempts at digitizing TOFs should keep in mind that digitization can be a time-consuming process, pilot testing in the new format is necessary even for a previously tested intervention, and the structure provided by facilitators in face-to-face training must be embedded into the format of digitized trainings.

  18. Curricular Innovation in the Surgery Clerkship: Can Assessment Methods Influence Development of Critical Thinking and Clinical Skills?

    Science.gov (United States)

    McClintic, James A; Snyder, Clifford L; Brown, Kimberly M

    2018-03-12

    Although key clinical skills have been defined in the Core Entrustable Professional Activities, there is a need to improve medical school curricula with standardized training opportunities and assessments of these skills. Thus, we aimed to develop an innovative curriculum that emphasized critical thinking and clinical skills. We hypothesized that we would be able to observe measurable improvement on assessments of students' critical thinking and clinical skills after the implementation of the new curriculum. Prospective, Quasi-Experimental study with the use of historical controls. This study took place through the third-year surgical clerkship at the University of Texas Medical Branch at the Galveston, Houston, and Austin, Texas, locations. A total of 214 students taking the third-year surgical clerkship for the first time during the periods of interest were included. Although the students with traditional curriculum improved 9.5% on a short answer exam from preclerkship to postclerkship completion, the students with new curriculum improved by 40%. Students under the new curriculum performed significantly better on the Objective Structured Clinical Exam; however, their shelf scores were lower. Under this new curriculum and grading system, we demonstrated that students can be incentivized to improve critical thinking and clinical skills, but this needs to be balanced with knowledge-based incentives. Copyright © 2018 Association of Program Directors in Surgery. Published by Elsevier Inc. All rights reserved.

  19. TA-GC cloning: A new simple and versatile technique for the directional cloning of PCR products for recombinant protein expression.

    Directory of Open Access Journals (Sweden)

    Athanasios Niarchos

    Full Text Available During the last few decades, the recombinant protein expression finds more and more applications. The cloning of protein-coding genes into expression vectors is required to be directional for proper expression, and versatile in order to facilitate gene insertion in multiple different vectors for expression tests. In this study, the TA-GC cloning method is proposed, as a new, simple and efficient method for the directional cloning of protein-coding genes in expression vectors. The presented method features several advantages over existing methods, which tend to be relatively more labour intensive, inflexible or expensive. The proposed method relies on the complementarity between single A- and G-overhangs of the protein-coding gene, obtained after a short incubation with T4 DNA polymerase, and T and C overhangs of the novel vector pET-BccI, created after digestion with the restriction endonuclease BccI. The novel protein-expression vector pET-BccI also facilitates the screening of transformed colonies for recombinant transformants. Evaluation experiments of the proposed TA-GC cloning method showed that 81% of the transformed colonies contained recombinant pET-BccI plasmids, and 98% of the recombinant colonies expressed the desired protein. This demonstrates that TA-GC cloning could be a valuable method for cloning protein-coding genes in expression vectors.

  20. TA-GC cloning: A new simple and versatile technique for the directional cloning of PCR products for recombinant protein expression.

    Science.gov (United States)

    Niarchos, Athanasios; Siora, Anastasia; Konstantinou, Evangelia; Kalampoki, Vasiliki; Lagoumintzis, George; Poulas, Konstantinos

    2017-01-01

    During the last few decades, the recombinant protein expression finds more and more applications. The cloning of protein-coding genes into expression vectors is required to be directional for proper expression, and versatile in order to facilitate gene insertion in multiple different vectors for expression tests. In this study, the TA-GC cloning method is proposed, as a new, simple and efficient method for the directional cloning of protein-coding genes in expression vectors. The presented method features several advantages over existing methods, which tend to be relatively more labour intensive, inflexible or expensive. The proposed method relies on the complementarity between single A- and G-overhangs of the protein-coding gene, obtained after a short incubation with T4 DNA polymerase, and T and C overhangs of the novel vector pET-BccI, created after digestion with the restriction endonuclease BccI. The novel protein-expression vector pET-BccI also facilitates the screening of transformed colonies for recombinant transformants. Evaluation experiments of the proposed TA-GC cloning method showed that 81% of the transformed colonies contained recombinant pET-BccI plasmids, and 98% of the recombinant colonies expressed the desired protein. This demonstrates that TA-GC cloning could be a valuable method for cloning protein-coding genes in expression vectors.