ADVANTAGES/DISADVANTAGES FOR ISCO METHODS IN-SITU FENTON OXIDATION IN-SITU PERMANGANATE OXIDATION
The advantages and disadvantages of in-situ Fenton oxidation and in-situ permanganate oxidation will be presented. This presentation will provide a brief overview of each technology and a detailed analysis of the advantages and disadvantages of each technology. Included in the ...
A Novel in situ Trigger Combination Method
International Nuclear Information System (INIS)
Buzatu, Adrian; Warburton, Andreas; Krumnack, Nils; Yao, Wei-Ming
2012-01-01
Searches for rare physics processes using particle detectors in high-luminosity colliding hadronic beam environments require the use of multi-level trigger systems to reject colossal background rates in real time. In analyses like the search for the Higgs boson, there is a need to maximize the signal acceptance by combining multiple different trigger chains when forming the offline data sample. In such statistically limited searches, datasets are often amassed over periods of several years, during which the trigger characteristics evolve and their performance can vary significantly. Reliable production cross-section measurements and upper limits must take into account a detailed understanding of the effective trigger inefficiency for every selected candidate event. We present as an example the complex situation of three trigger chains, based on missing energy and jet energy, to be combined in the context of the search for the Higgs (H) boson produced in association with a W boson at the Collider Detector at Fermilab (CDF). We briefly review the existing techniques for combining triggers, namely the inclusion, division, and exclusion methods. We introduce and describe a novel fourth in situ method whereby, for each candidate event, only the trigger chain with the highest a priori probability of selecting the event is considered. The in situ combination method has advantages of scalability to large numbers of differing trigger chains and of insensitivity to correlations between triggers. We compare the inclusion and in situ methods for signal event yields in the CDF WH search.
An improved in situ method for determining depth distributions of gamma-ray emitting radionuclides
International Nuclear Information System (INIS)
Benke, R.R.; Kearfott, K.J.
2001-01-01
In situ gamma-ray spectrometry determines the quantities of radionuclides in some medium with a portable detector. The main limitation of in situ gamma-ray spectrometry lies in determining the depth distribution of radionuclides. This limitation is addressed by developing an improved in situ method for determining the depth distributions of gamma-ray emitting radionuclides in large area sources. This paper implements a unique collimator design with conventional radiation detection equipment. Cylindrically symmetric collimators were fabricated to allow only those gamma-rays emitted from a selected range of polar angles (measured off the detector axis) to be detected. Positioned with its axis normal to surface of the media, each collimator enables the detection of gamma-rays emitted from a different range of polar angles and preferential depths. Previous in situ methods require a priori knowledge of the depth distribution shape. However, the absolute method presented in this paper determines the depth distribution as a histogram and does not rely on such assumptions. Other advantages over previous in situ methods are that this method only requires a single gamma-ray emission, provides more detailed depth information, and offers a superior ability for characterizing complex depth distributions. Collimated spectrometer measurements of buried area sources demonstrated the ability of the method to yield accurate depth information. Based on the results of actual measurements, this method increases the potential of in situ gamma-ray spectrometry as an independent characterization tool in situations with unknown radionuclide depth distributions
Nuclear methods for 'in-situ' geophysical investigations. 2
International Nuclear Information System (INIS)
Russell, J.P.; Oliver, D.W.
1985-01-01
There has been considerable effort expended by governmental, institutional, and private agencies in the development of suitable materials and instruments for application of nuclear methods for exploration and evaluation of mineral, oil, and gas resources. As a consequence of the peculiar circumstances of in situ investigations, much of the apparatus is, of design and construction, specifically adapted to the environment of the borehole (limited lateral dimension, high temperature and pressure, deep boreholes, etc.) which differs considerably from laboratory conditions. Meeting the needs for reliability and stability under adverse conditions of operation has led to often unique and innovative instrumentation. Much of the development effort has been provided by commercial service organizations whose interests require many details be maintained as proprietary. In addition, the tailoring of services to meet varying environmental and informational constraints has led to a wide range of instrument designs. As a result, it is difficult to provide an exhaustive description of all apparatus available for in situ investigations, rather, in this instance, systems and subsystems will be generically discussed. Radiation sources, detectors, and borehole probe designs are covered. (Auth.)
Semiconductor/metal nanocomposites formed by in situ reduction method in multilayer thin films
International Nuclear Information System (INIS)
Song Yanli; Wang Enbo; Tian Chungui; Mao Baodong; Wang Chunlei
2009-01-01
A layer-by-layer adsorption and in situ reduction method was adopted for synthesizing semiconductor/metal nanocomposites in multilayer ultra-thin films. Alternate adsorption of ZnO nanoparticles modified with poly(ethyleneimine), hydrogentetrachloroaurate and poly(styrenesulfonate) sodium results in the formation of ZnO/AuCl 4 - -loaded multilayer films. In situ reduction of the incorporated metal ions by heating yields ZnO/Au nanocomposites in the films. UV-vis absorption spectroscopy and X-ray photoelectron spectroscopy were used to characterize the components of the composite films. UV-vis spectra indicate regular growth of the films. The electrochemistry behavior of the multilayer films was studied in detail on indium tin oxide electrode. The combined results suggest that the layer-by-layer adsorption and subsequent reduction method used here provides an effective way to synthesize ZnO/Au nanocomposites in the polymer matrix
Improvements in in-situ filter test methods using a total light-scattering detector
International Nuclear Information System (INIS)
Marshall, M.; Stevens, D.C.
1986-01-01
This paper presents research aimed at providing useful data on a commonly used technique; a DOP (di-2-ethylhexyl phthalate) aerosol and a total light-scattering photometer. Methods of increasing the sensitivity of this technique are described. Alternative methods of in-situ filter testing are also considered. The sensitivity of a typical, modern, total light-scattering photometer, as a function of particle diameter, has a broad maximum in mass terms between 0.1 and 0.4 um. At its maximum usable sensitivity the instrument can detect approx. 1 particle/cm 3 . This response can be explained by light scattering theory and particle loss in the instrument inlet. The mass median diameter of the aerosols produced by various DOP generators varies from 0.2 to 1.0μm. Experiments with good quality HEPA filters indicate a maximum penetration for particles of 0.15 - 0.2μm. Details of the studies are given and the consequences discussed. It is shown that filter penetration of -3 % can be measured in-situ with existing equipment. Methods of extending the sensitivity to measure a penetration of approx.10 -5 % are described. (author)
International Nuclear Information System (INIS)
Song, Yingze; Yu, Jinhong; Dai, Dan; Song, Lixian; Jiang, Nan
2014-01-01
Highlights: • In-situ and ex-situ methods were applied to modify silica particles. • In-situ method was more beneficial to preparing silica particles with high BET surface area. • Silicone rubber filled with in-situ modified silica exhibits excellent mechanical and thermal properties. - Abstract: In-situ and ex-situ methods were applied to modify silica particles in order to investigate their effects on the reinforcement of silicone rubber. Surface area and pore analyzer, laser particle size analyzer, Fourier-transform infrared spectroscopy (FTIR), contact-angle instrument, and transmission electron microscope (TEM) were utilized to investigate the structure and properties of the modified silica particles. Dynamic mechanical thermal analyzer (DMTA) was employed to characterize the vulcanizing behavior and mechanical properties of the composites. Thermogravimetric analysis (TGA) was performed to test the thermal stability of the composites. FTIR and contact angle analysis indicated that silica particles were successfully modified by these two methods. The BET surface area and TEM results reflected that in-situ modification was more beneficial to preparing silica particles with irregular shape and higher BET surface area in comparison with ex-situ modification. The DMTA and TGA data revealed that compared with ex-situ modification, the in-situ modification produced positive influence on the reinforcement of silicone rubber
Cost accounting method for in-situ leaching mines and its application
International Nuclear Information System (INIS)
Cheng Zongfang; Yang Yihan; Liu Zhanxiang; Lai Yongchun
2008-01-01
Cost structures and accounting method for in-situ leaching mines are studied according to the technical characteeristics of in-situ leaching. A method of cost forecast for in-situ leaching deposit or mine area is presented, and the application of this method is illustrated with examples. (authors)
Bentrup, Ursula
2010-12-01
Several in situ techniques are known which allow investigations of catalysts and catalytic reactions under real reaction conditions using different spectroscopic and X-ray methods. In recent years, specific set-ups have been established which combine two or more in situ methods in order to get a more detailed understanding of catalytic systems. This tutorial review will give a summary of currently available set-ups equipped with multiple techniques for in situ catalyst characterization, catalyst preparation, and reaction monitoring. Besides experimental and technical aspects of method coupling including X-ray techniques, spectroscopic methods (Raman, UV-vis, FTIR), and magnetic resonance spectroscopies (NMR, EPR), essential results will be presented to demonstrate the added value of multitechnique in situ approaches. A special section is focussed on selected examples of use which show new developments and application fields.
DEFF Research Database (Denmark)
Gutberlet, Thomas; Klösgen, Beate Maria; Krastev, Rumen
2004-01-01
variation. It was observed that the method was capable of visualizing the adsorption of phospholipid layers to different solid-liquid interfaces and to resolve structural details at Angstroem resolution. The results depended strongly on a sufficiently good signal-to-noise ratio of the specific measurements......The use of neutron reflectivity as a method to study in-situ adsorption of phospholipid layers to solid-liquid interfaces was analyzed. The most important advantage of neutron reflectometry is the possibility to very the refractive index of the specific sample by isotope exchange, called contrast...
Advanced hydraulic fracturing methods to create in situ reactive barriers
International Nuclear Information System (INIS)
Murdoch, L.
1997-01-01
This article describes the use of hydraulic fracturing to increase permeability in geologic formations where in-situ remedial action of contaminant plumes will be performed. Several in-situ treatment strategies are discussed including the use of hydraulic fracturing to create in situ redox zones for treatment of organics and inorganics. Hydraulic fracturing methods offer a mechanism for the in-situ treatment of gently dipping layers of reactive compounds. Specialized methods using real-time monitoring and a high-energy jet during fracturing allow the form of the fracture to be influenced, such as creation of assymmetric fractures beneath potential sources (i.e. tanks, pits, buildings) that should not be penetrated by boring. Some examples of field applications of this technique such as creating fractures filled with zero-valent iron to reductively dechlorinate halogenated hydrocarbons, and the use of granular activated carbon to adsorb compounds are discussed
Monte Carlo methods beyond detailed balance
Schram, Raoul D.; Barkema, Gerard T.|info:eu-repo/dai/nl/101275080
2015-01-01
Monte Carlo algorithms are nearly always based on the concept of detailed balance and ergodicity. In this paper we focus on algorithms that do not satisfy detailed balance. We introduce a general method for designing non-detailed balance algorithms, starting from a conventional algorithm satisfying
Energy Technology Data Exchange (ETDEWEB)
Reinke, M.; Mantzaras, I.; Schaeren, R.; Bombach, R.; Inauen, A.; Schenker, S.
2003-03-01
The catalytic reactivity of methane over platinum at pressures of up to 14 bar was evaluated with in-situ Raman measurements and detailed numerical predictions from two different heterogeneous chemical reaction schemes. The best agreement to the measurements was achieved with Deutschmann's reaction scheme that yielded the correct trend for the pressure dependence of the catalytic reactivity, although in absolute terms the reactivity was overpredicted. The catalytic reactivity was consistently underpredicted at all pressures with the reaction scheme of Vlachos. (author)
Characterization of VPO ammoxidation catalysts by in situ methods
Energy Technology Data Exchange (ETDEWEB)
Martin, A.; Luecke, B.; Brueckner, A.; Steinike, U. [Institut fuer Angewandte Chemie Berlin-Adlershof e.V., Berlin (Germany); Brzezinka, K.W. [Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin (Germany); Meisel, M. [Humboldt-Universitaet, Berlin (Germany). Inst. fuer Chemie
1998-12-31
In-situ methods are well known as powerful tools in studying catalyst formation processes, their solid state properties under working conditions and the interaction with the feed, intermediates and products to reveal reaction mechanisms. This paper gives a short overview on results of intense studies using in-situ techniques to reveal VPO catalyst generation processes, interaction of educts, intermediates and products with VPO catalyst surfaces and mechanistic insights. Catalytic data of the ammoxidation of toluene on different VPOs complete these findings. The precursor-catalyst transformation processes were preferently investigated by in-situ XRD, in-situ Raman and in-situ ESR spectroscopy. The interaction of aromatic molecules and intermediates, resp., and VPO solid surfaces was followed by in-situ ESR and in-situ FTIR spectroscopy. Mechanistic information was mainly obtained using in-situ FTIR spectroscopy and the temporal-analysis-of-products (TAP) technique. Catalytic studies were carried out in a fixed-bed microreactor on pure (NH{sub 4}){sub 2}(VO){sub 3}(P{sub 2}O{sub 7}){sub 2}, generated [(NH{sub 4}){sub 2}(VO{sub 3})(P{sub 2}O{sub 7}){sub 2}+V{sub x}O{sub y}] catalysts, having different V{sub x}O{sub y} proportions by use of VOHPO{sub 4} x 1/2H{sub 2}O (V/P=1) and recently studied (VO){sub 3}(PO{sub 4}){sub 2} x 7 H{sub 2}O (V/P=1.5) precursors; the well-known (VO){sub 2}P{sub 2}O{sub 7} was used for comparison. (orig.)
Experiments at Scale with In-Situ Visualization Using ParaView/Catalyst in RAGE
Energy Technology Data Exchange (ETDEWEB)
Kares, Robert John [Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
2014-10-31
In this paper I describe some numerical experiments performed using the ParaView/Catalyst in-situ visualization infrastructure deployed in the Los Alamos RAGE radiation-hydrodynamics code to produce images from a running large scale 3D ICF simulation on the Cielo supercomputer at Los Alamos. The detailed procedures for the creation of the visualizations using ParaView/Catalyst are discussed and several images sequences from the ICF simulation problem produced with the in-situ method are presented. My impressions and conclusions concerning the use of the in-situ visualization method in RAGE are discussed.
Solid oxide electrode kinetics in light of in situ surface studies
DEFF Research Database (Denmark)
Mogensen, Mogens Bjerg
2014-01-01
The combination of in situ and in particular in operando characterization methods such as electrochemical impedance spectroscopy (EIS) on both technical and model electrode are well known ways to gain some practical insight in electrode reaction kinetics. Yet, is has become clear that in spite...... of the strengths it is not sufficient to reveal much details of the electrode mechanisms mainly because it provide average values only. Therefore it has to be combined with surface science methods in order to reveal the interface structure and composition. Ex situ methods have been very useful over the latest....... Furthermore, it seems that detailed mathematical modeling using new tools like COMSOL is necessary for the synthesis of the large amount of data for a well-characterized electrode into one physical meaningful picture. A brief review of literature an own data will be presented with a practical example of SOFC...
International Nuclear Information System (INIS)
Rostron, Peter D.; Ramsey, Michael H.; Heathcote, John A.
2012-01-01
In the UK, it is estimated that there may be 20,000,000 cubic metres of contaminated land at Sellafield alone. Harwell and Dounreay are known to have significant amounts of radioactive or nonradioactive contaminated land (NDA, 2006). It is therefore important to devise optimal methods for the characterisation of areas of land for radionuclide content, in order to enable cost-effective decommissioning. With chemical contaminants, ex situ measurements are made on a larger volume of soil than are in situ measurements, such as PXRF. However, the opposite is often true for the characterisation of radioactive contamination, when this involves the detection of penetrating radiation from γ-emitting radionuclides. This means that when investigating for hotspots of radioactive contamination at or near the ground surface, better coverage can be obtained using in situ methods. This leads to the question, what is the optimal strategy (e.g. percentage coverage, counting time) for in situ characterisation of radioactively contaminated land' Surveys on light-moderate contaminated areas of ground were conducted at Dounreay in order to compare the relative effectiveness of in situ and ex situ methods, both for the detection of radioactive hotspots and also for estimating the average radionuclide content of an area of ground. These surveys suggest that continuous coverage by in situ devices is more effective at hotspot detection, with ex situ laboratory measurements being less effective, although in one case elevated activity below 10 cm depth that was identified by ex situ measurement was not located by in situ measurement. The surveys also highlighted that careful choice of an appropriate spatial model is critical to the estimation of activity concentrations over averaging areas. Whereas continuous coverage may be considered necessary for hotspot identification, in the particular case of the detection of hot particles (where the particle is very small compared to the sampling
Benke, R R
2002-01-01
In situ gamma-ray spectrometry uses a portable detector to quantify radionuclides in materials. The main shortcoming of in situ gamma-ray spectrometry has been its inability to determine radionuclide depth distributions. Novel collimator designs were paired with a commercial in situ gamma-ray spectrometry system to overcome this limitation for large area sources. Positioned with their axes normal to the material surface, the cylindrically symmetric collimators limited the detection of un attenuated gamma-rays from a selected range of polar angles (measured off the detector axis). Although this approach does not alleviate the need for some knowledge of the gamma-ray attenuation characteristics of the materials being measured, the collimation method presented in this paper represents an absolute method that determines the depth distribution as a histogram, while other in situ methods require a priori knowledge of the depth distribution shape. Other advantages over previous in situ methods are that this method d...
Pharmaceutical drug detailing in primary care: extent and methods
DEFF Research Database (Denmark)
Schramm, Jesper
The dimension and methods of pharmaceutical marketing towards 47 danish general practitioners is described. Conclusions: Pharmaceutical drug detailing possesses a huge potential to influence the prescription pattern of GP's.......The dimension and methods of pharmaceutical marketing towards 47 danish general practitioners is described. Conclusions: Pharmaceutical drug detailing possesses a huge potential to influence the prescription pattern of GP's....
In situ methods for Li-ion battery research: A review of recent developments
Harks, P. P. R. M. L.; Mulder, F. M.; Notten, P. H. L.
2015-08-01
A considerable amount of research is being directed towards improving lithium-ion batteries in order to meet today's market demands. In particular in situ investigations of Li-ion batteries have proven extremely insightful, but require the electrochemical cell to be fully compatible with the conditions of the testing method and are therefore often challenging to execute. Advantageously, in the past few years significant progress has been made with new, more advanced, in situ techniques. Herein, a comprehensive overview of in situ methods for studying Li-ion batteries is given, with the emphasis on new developments and reported experimental highlights.
Comparative study of in-situ filter test methods
International Nuclear Information System (INIS)
Marshall, M.; Stevens, D.C.
1981-01-01
Available methods of testing high efficiency particulate aerosol (HEPA) filters in-situ have been reviewed. In order to understand the relationship between the results produced by different methods a selection has been compared. Various pieces of equipment for generating and detecting aerosols have been tested and their suitability assessed. Condensation-nuclei, DOP (di-octyl phthalate) and sodium-flame in-situ filter test methods have been studied, using the 500 cfm (9000 m 3 /h) filter test rig at Harwell and in the field. Both the sodium-flame and DOP methods measure the penetration through leaks and filter material. However the measured penetration through filtered leaks depends on the aerosol size distribution and the detection method. Condensation-nuclei test methods can only be used to measure unfiltered leaks since condensation nuclei have a very low penetration through filtered leaks. A combination of methods would enable filtered and unfiltered leaks to be measured. A condensation-nucleus counter using n-butyl alcohol as the working fluid has the advantage of being able to detect any particle up to 1 μm in diameter, including DOP, and so could be used for this purpose. A single-particle counter has not been satisfactory because of interference from particles leaking into systems under extract, particularly downstream of filters, and because the concentration of the input aerosol has to be severely limited. The sodium-flame method requires a skilled operator and may cause safety and corrosion problems. The DOP method using a total light scattering detector has so far been the most satisfactory. It is fairly easy to use, measures reasonably low values of penetration and gives rapid results. DOP has had no adverse effect on HEPA filters over a long series of tests
Directory of Open Access Journals (Sweden)
Danijel Karolyi
2010-01-01
Full Text Available Fermentation characteristics of grass and grass silage at different maturities were studied using in situ and in vitro gas production methods. In situ data determined difference between grass and silage. Degradable fraction decreased as grass matured while the undegradable fraction increased. Rate of degradation (kd was slower for silage than fresh grass. Gas production method (GP data showed that fermentation of degradable fraction was different between stage of maturity in both grass and silage. Other data did not show any difference with the exception for the rate of GP of soluble and undegradable fraction. The in situ degradation characteristics were estimated from GP characteristics. The degradable and undegradable fractions could be estimated by multiple relationships. Using the three-phases model for gas production kd and fermentable organic matter could be estimated from the same parameters. The only in situ parameter that could not be estimated with GP parameters was the soluble fraction. The GP method and the three phases model provided to be an alternative to the in situ method for animal feed evaluations.
In-situ spectrometry of 137Cs in the soil by unfolding method
International Nuclear Information System (INIS)
Fueloep, M.; Ragan, P.; Krnac, S.
1995-01-01
This contribution is aimed to the possibility of improving the in-situ gamma spectrometry to be independent on a knowledge about a depth distribution of 137 Cs in soil and sufficiently sensitive for the measurement of the post-Chernobyl 137 Cs at present, as well. The depth distribution of 137 Cs averaged over a large area of soil is obtained by unfolding of the detector responses to primary and in soil forward scattered photons. The proposed method employs detector with and without collimator. The 137 Cs distributions obtained in-situ measurements are analysed, and comparisons are made to the results obtained with soil sampling and with standard in-situ spectrometry, as well. 5 figs., 1 tab., 4 refs
The potential of high resolution ultrasonic in-situ methods
International Nuclear Information System (INIS)
Schuster, K.
2010-01-01
source and receivers and on the applied frequencies. With the help of identified phases in the ultrasonic wave field, their travel times and appropriate amplitudes kinematic and dynamic parameters are derived routinely. The most important are: P-wave velocity (longitudinal or compressional wave velocity (vp)), Sv-wave velocity (vertical polarised transversal or shear wave velocity (vsv), absolute amplitudes of first arrival (P-wave) and Sv-wave onset phases, normalised amplitudes of first arrival (P-wave) and Sv-wave onset phases, apparent frequency of first arrival phases (P-wave) and Sv-wave onset phases, in-situ dynamic elastic Poisson's ratio, in-situ dynamic elastic Young's modulus and in-situ dynamic elastic modulus of rigidity. The main qualities which were detected and/or characterised with the help of these parameters in the past years are: - Degree and extent of EDZ/EdZ. - Long and short term development of EDZ/EdZ (application of repetition measurements). - Borehole disturbed Zones (BdZ). - Small scale rock heterogeneities. - Seismic anisotropy. - Stress orientation via rotational interval velocity measurements. - Determination of dynamic elastic in-situ parameters. Results from repetition measurements showed the high reliability of the methods. Furthermore, in several experiments the ultrasonic methods were combined with other geotechnical or geophysical methods, for example permeability and geo-electrical methods. Results from a seismic anisotropy study at the Mont Terri Rock Laboratory are shown. Two sub-horizontal 2 m long boreholes with a distance of 1 m were used. Nearly 600 different ray paths were analysed. Depending on the angles of the ray paths (60 deg. -300 deg. and 120 deg. -240 deg. ) the P-wave velocities vary between 2600 and 3100 m/s. Lower velocities are supposed to be influenced by the EDZ. (authors)
Method for in situ carbon deposition measurement for solid oxide fuel cells
Kuhn, J.; Kesler, O.
2014-01-01
Previous methods to measure carbon deposition in solid oxide fuel cell (SOFC) anodes do not permit simultaneous electrochemical measurements. Electrochemical measurements supplemented with carbon deposition quantities create the opportunity to further understand how carbon affects SOFC performance and electrochemical impedance spectra (EIS). In this work, a method for measuring carbon in situ, named here as the quantification of gasified carbon (QGC), was developed. TGA experiments showed that carbon with a 100 h residence time in the SOFC was >99.8% gasified. Comparison of carbon mass measurements between the TGA and QGC show good agreement. In situ measurements of carbon deposition in SOFCs at varying molar steam/carbon ratios were performed to further validate the QGC method, and suppression of carbon deposition with increasing steam concentration was observed, in agreement with previous studies. The technique can be used to investigate in situ carbon deposition and gasification behavior simultaneously with electrochemical measurements for a variety of fuels and operating conditions, such as determining conditions under which incipient carbon deposition is reversible.
In-situ thermoelectrochemistry working with heated electrodes
Gründler, Peter
2015-01-01
This book represents the first rigorous treatment of thermoelectrochemistry, providing an overview that will stimulate electrochemists to develop and apply modern thermoelectrochemical methods. While classical static approaches are also covered, the emphasis lies on methods that make it possible to independently vary temperature such as in-situ heating of electrodes by means of electric current, microwaves or lasers. For the first time, "hot-wire electrochemistry" is examined in detail. The theoretical background presented addresses all aspects of temperature impacts in the context of electroc
In situ thermal properties characterization using frequential methods
Energy Technology Data Exchange (ETDEWEB)
Carpentier, O.; Defer, D.; Antczak, E.; Chauchois, A.; Duthoit, B. [Laboratoire dArtois de Mecanique Thermique Instrumentation (LAMTI), FSA Universite dArtois, Technoparc Futura, 62400 Bethune (France)
2008-07-01
In numerous fields, especially that of geothermal energy, we need to know about the thermal behaviour of the soil now that the monitoring of renewable forms of energy is an ecological, economic and scientific issue. Thus heat from the soil is widely used for air-conditioning systems in buildings both in Canada and in the Scandinavian countries, and it is spreading. The effectiveness of this technique is based on the soils calorific potential and its thermophysical properties which will define the quality of the exchanges between the soil and a heat transfer fluid. This article puts forward a method to be used for the in situ thermophysical characterisation of a soil. It is based upon measuring the heat exchanges on the surface of the soil and on measuring a temperature a few centimetres below the surface. The system is light, inexpensive, well-suited to the taking of measurements in situ without the sensors used introducing any disturbance into the heat exchanges. Whereas the majority of methods require excitation, the one presented here is passive and exploits natural signals. Based upon a few hours of recording, the natural signals allow us to identify the soils thermophysical properties continuously. The identification is based upon frequency methods the quality of which can be seen when the thermophysical properties are injected into a model with finite elements by means of a comparison of the temperatures modelled and those actually measured on site. (author)
Quantitative comparison of in situ soil CO2 flux measurement methods
Jennifer D. Knoepp; James M. Vose
2002-01-01
Development of reliable regional or global carbon budgets requires accurate measurement of soil CO2 flux. We conducted laboratory and field studies to determine the accuracy and comparability of methods commonly used to measure in situ soil CO2 fluxes. Methods compared included CO2...
In situ feeding rates of plantonic copepods: A comparison of four methods
DEFF Research Database (Denmark)
Kiørboe, Thomas; Møhlenberg, Flemming; Riisgård, Hans Ulrik
1985-01-01
into estimates of in situ algal grazing rates by means of independently estimated gut turnover times, and were compared with chlorophyll and particle-volume grazing rates of animals sampled simultaneously and incubated in water from the collection depth. In addition, egg-production rates of adult females were...... problems of the different methods are discussed, and it is concluded that they all approach representative (although minimum) estimates of in situ feeding rates....
Signal processing methods for in-situ creep specimen monitoring
Guers, Manton J.; Tittmann, Bernhard R.
2018-04-01
Previous work investigated using guided waves for monitoring creep deformation during accelerated life testing. The basic objective was to relate observed changes in the time-of-flight to changes in the environmental temperature and specimen gage length. The work presented in this paper investigated several signal processing strategies for possible application in the in-situ monitoring system. Signal processing methods for both group velocity (wave-packet envelope) and phase velocity (peak tracking) time-of-flight were considered. Although the Analytic Envelope found via the Hilbert transform is commonly applied for group velocity measurements, erratic behavior in the indicated time-of-flight was observed when this technique was applied to the in-situ data. The peak tracking strategies tested had generally linear trends, and tracking local minima in the raw waveform ultimately showed the most consistent results.
Methods and systems for in-situ electroplating of electrodes
Zappi, Guillermo Daniel; Zarnoch, Kenneth Paul; Huntley, Christian Andrew; Swalla, Dana Ray
2015-06-02
The present techniques provide electrochemical devices having enhanced electrodes with surfaces that facilitate operation, such as by formation of a porous nickel layer on an operative surface, particularly of the cathode. The porous metal layer increases the surface area of the electrode, which may result in increasing the efficiency of the electrochemical devices. The formation of the porous metal layer is performed in situ, that is, after the assembly of the electrodes into an electrochemical device. The in situ process offers a number of advantages, including the ability to protect the porous metal layer on the electrode surface from damage during assembly of the electrochemical device. The enhanced electrode and the method for its processing may be used in any number of electrochemical devices, and is particularly well suited for electrodes in an electrolyzer useful for splitting water into hydrogen and oxygen.
In-situ spectrometry of {sup 137}Cs in the soil by unfolding method
Energy Technology Data Exchange (ETDEWEB)
Fueloep, M; Ragan, P [Inst. of Preventive and Clinical Medicine, 833301 Bratislava (Slovakia); Krnac, S [Slovak Technical Univ., Bratislava (Slovakia)
1996-12-31
This contribution is aimed to the possibility of improving the in-situ gamma spectrometry to be independent on a knowledge about a depth distribution of {sup 137}Cs in soil and sufficiently sensitive for the measurement of the post-Chernobyl {sup 137}Cs at present, as well. The depth distribution of {sup 137}Cs averaged over a large area of soil is obtained by unfolding of the detector responses to primary and in soil forward scattered photons. The proposed method employs detector with and without collimator. The {sup 137}Cs distributions obtained in-situ measurements are analysed, and comparisons are made to the results obtained with soil sampling and with standard in-situ spectrometry, as well. 5 figs., 1 tab., 4 refs.
A method to provide rapid in situ determination of tip radius in dynamic atomic force microscopy
International Nuclear Information System (INIS)
Santos, Sergio; Guang Li; Souier, Tewfik; Gadelrab, Karim; Chiesa, Matteo; Thomson, Neil H.
2012-01-01
We provide a method to characterize the tip radius of an atomic force microscopy in situ by monitoring the dynamics of the cantilever in ambient conditions. The key concept is that the value of free amplitude for which transitions from the attractive to repulsive force regimes are observed, strongly depends on the curvature of the tip. In practice, the smaller the value of free amplitude required to observe a transition, the sharper the tip. This general behavior is remarkably independent of the properties of the sample and cantilever characteristics and shows the strong dependence of the transitions on the tip radius. The main advantage of this method is rapid in situ characterization. Rapid in situ characterization enables one to continuously monitor the tip size during experiments. Further, we show how to reproducibly shape the tip from a given initial size to any chosen larger size. This approach combined with the in situ tip size monitoring enables quantitative comparison of materials measurements between samples. These methods are set to allow quantitative data acquisition and make direct data comparison readily available in the community.
Formation factor logging in-situ by electrical methods. Background and methodology
International Nuclear Information System (INIS)
Loefgren, Martin; Neretnieks, Ivars
2002-10-01
Matrix diffusion has been identified as one of the most important mechanisms governing the retardation of radionuclides escaping from a deep geological repository for nuclear waste. Radionuclides dissolved in groundwater flowing in water-bearing fractures will diffuse into water filled micropores in the rock. Important parameters governing the matrix diffusion are the formation factor, the surface diffusion and sorption. This report focuses on the formation factor in undisturbed intrusive igneous rock and the possibility of measuring this parameter in-situ. The background to and the methodology of formation factor logging in-situ by electrical methods are given. The formation factor is here defined as a parameter only depending on the geometry of the porous system and not on the diffusing specie. Traditionally the formation factor has been measured by through diffusion experiments on core samples, which are costly and time consuming. It has been shown that the formation factor could also be measured by electrical methods that are faster and less expensive. Previously this has only been done quantitatively in the laboratory on a centimetre or decimetre scale. When measuring the formation factor in-situ in regions with saline groundwater only the rock resistivity and the pore water resistivity are needed. The rock resistivity could be obtained by a variety of geophysical downhole tools. Water-bearing fractures disturb the measurements and data possibly affected by free water has to be sorted out. This could be done without loosing too much data if the vertical resolution of the tool is high enough. It was found that the rock resistivity tool presently used by SKB are neither quantitative or have enough vertical resolution. Therefore the slimhole Dual-Laterolog from Antares was tested with good results. This tool has a high vertical resolution and gives quantitative rock resistivities that need no correction. At present there is no method of directly obtaining the
Formation factor logging in-situ by electrical methods. Background and methodology
Energy Technology Data Exchange (ETDEWEB)
Loefgren, Martin; Neretnieks, Ivars [Royal Inst. of Tech., Stockholm (Sweden). Dept. of Chemical Engineering and Technology
2002-10-01
Matrix diffusion has been identified as one of the most important mechanisms governing the retardation of radionuclides escaping from a deep geological repository for nuclear waste. Radionuclides dissolved in groundwater flowing in water-bearing fractures will diffuse into water filled micropores in the rock. Important parameters governing the matrix diffusion are the formation factor, the surface diffusion and sorption. This report focuses on the formation factor in undisturbed intrusive igneous rock and the possibility of measuring this parameter in-situ. The background to and the methodology of formation factor logging in-situ by electrical methods are given. The formation factor is here defined as a parameter only depending on the geometry of the porous system and not on the diffusing specie. Traditionally the formation factor has been measured by through diffusion experiments on core samples, which are costly and time consuming. It has been shown that the formation factor could also be measured by electrical methods that are faster and less expensive. Previously this has only been done quantitatively in the laboratory on a centimetre or decimetre scale. When measuring the formation factor in-situ in regions with saline groundwater only the rock resistivity and the pore water resistivity are needed. The rock resistivity could be obtained by a variety of geophysical downhole tools. Water-bearing fractures disturb the measurements and data possibly affected by free water has to be sorted out. This could be done without loosing too much data if the vertical resolution of the tool is high enough. It was found that the rock resistivity tool presently used by SKB are neither quantitative or have enough vertical resolution. Therefore the slimhole Dual-Laterolog from Antares was tested with good results. This tool has a high vertical resolution and gives quantitative rock resistivities that need no correction. At present there is no method of directly obtaining the
Laser Based In Situ Techniques: Novel Methods for Generating Extreme Conditions in TEM Samples
Energy Technology Data Exchange (ETDEWEB)
Taheri, M; Lagrange, T; Reed, B; Armstrong, M; Campbell, G; DeHope, W; Kim, J; King, W; Masiel, D; Browning, N
2008-02-25
The Dynamic Transmission Electron Microscope (DTEM) is introduced as a novel tool for in situ processing of materials. Examples of various types of dynamic studies outline the advantages and differences of laser-based heating in the DTEM in comparison to conventional (resistive) heating in situ TEM methods. We demonstrate various unique capabilities of the drive laser, namely, in situ processing of nanoscale materials, rapid and high temperature phase transformations, and controlled thermal activation of materials. These experiments would otherwise be impossible without the use of the DTEM drive laser. Thus, the potential of the DTEM to as a new technique to process and characterize the growth of a myriad of micro and nanostructures is demonstrated.
PROSCARA Inc. in-situ burning summary paper
International Nuclear Information System (INIS)
1994-06-01
In-situ burning as a viable response tactic in the event of an oil spill, was discussed. Key factors which influence a decision to use burning were enumerated, including a detailed analysis of the environmental effects of in-situ burning on soils. The critical parameters were time, soil heating and extent of oil penetration into the soil. It was noted that on water-saturated and frozen soil in-situ burning had no adverse effects. The advantages and disadvantages of in-situ burning vis-a-vis conventional mechanical recovery were discussed. Factors that do, and factors that do not support decisions in favour of in-situ burning were listed. 4 refs., 2 tabs
Wang, Peifang; Liu, Cui; Yao, Yu; Wang, Chao; Wang, Teng; Yuan, Ye; Hou, Jun
2017-05-01
To assess the capabilities of the different techniques in predicting Cadmium (Cd) bioavailability in Cd-contaminated soils with the addition of Zn, one in situ technique (diffusive gradients in thin films; DGT) was compared with soil solution concentration and four widely used single-step extraction methods (acetic acid, EDTA, sodium acetate and CaCl 2 ). Wheat and maize were selected as tested species. The results demonstrated that single Cd-polluted soils inhibited the growth of wheat and maize significantly compared with control plants; the shoot and root biomasses of the plants both dropped significantly (P 0.9) between Cd concentrations in two plants and Cd bioavailability indicated by each method in soils. Consequently, the results indicated that the DGT technique could be regarded as a good predictor of Cd bioavailability to plants, comparable to soil solution concentration and the four single-step extraction methods. Because the DGT technique can offer in situ data, it is expected to be widely used in more areas.
Lifescience Database Archive (English)
Full Text Available List Contact us Plabrain DB Image files of planarians analyzed by in situ hybridication and immunohistochemical... staining Data detail Data name Image files of planarians analyzed by in situ hybridication and immunohistochemical...sion patterns by whole-mount in situ hybridication and also protein distribution by immunohistochemical...Images are displayed in A list of image files of planarians analyzed by in situ hybridication and immunohistochemical...le search URL - Data acquisition method Whole-mount in situ hybridication, immunohistochemical staining Data
Remediation by in-situ solidification/stabilisation of Ardeer landfill, Scotland
International Nuclear Information System (INIS)
Wyllie, M.; Esnault, A.; Barker, P.
1997-01-01
The Ardeer Landfill site at ICI Explosives factory on the west coast of Scotland had been a repository for waste from the site for 40 years. In order to safeguard the local environment ICI Explosives, with approval of Local Authorities and the Clyde River Purification Board put into action a programme of investigation and planning which culminated in the in-situ treatment of 10,000 m3 of waste within the landfill by a deep mixing method using the open-quotes Colmixclose quotes system. The paper describes in varying degrees of detail the remediation from investigation to the execution of the in-situ stabilisation and presents the post construction monitoring results
Evaluation and selection of in-situ leaching mining method using analytic hierarchy process
International Nuclear Information System (INIS)
Zhao Heyong; Tan Kaixuan; Liu Huizhen
2007-01-01
According to the complicated conditions and main influence factors of in-situ leaching min- ing, a model and processes of analytic hierarchy are established for evaluation and selection of in-situ leaching mining methods based on analytic hierarchy process. Taking a uranium mine in Xinjiang of China for example, the application of this model is presented. The results of analyses and calculation indicate that the acid leaching is the optimum project. (authors)
In-situ reactive of x-ray optics by glow discharge
International Nuclear Information System (INIS)
Johnson, E.D.; Garrett, R.F.
1987-01-01
We have developed a method of in-situ reactive glow discharge cleaning of x-ray optical surfaces which is capable of complete removal of carbon contamination. Our work is the first to successfully clean an entire optical system in-situ and characterize its performance at short wavelengths (as low as 10 /angstrom/). The apparatus required is quite simple and can easily be fitted to most existing UHV (ultra high vacuum) mirror boxes of monochromators. The advantages of this technique over previously available methods include dramatic improvements in instrument performance and reductions in down time since the whole process typically takes a few days. This paper will briefly describe our results and detail the experimental considerations for application of the technique on different monochromator geometries. Possible improvements and extensions of the technique are also discussed
In-situ gamma spectrometry method for determination of environmental gamma dose
International Nuclear Information System (INIS)
Conti, Claudio de Carvalho
1995-07-01
This work tries to establish a methodology for germanium detectors calibration, normally used for in situ gamma ray spectrometry, for determining the environmental exposure rate in function of the energy of the incident photons. For this purpose a computer code has been developed, based on the stripping method, for the computational spectra analysis to calculate the contribution of the partial absorption of the gamma rays (Compton effect) in the active and nonactive parts of the detector. The resulting total absorption spectrum is then converted to fluence distribution in function of the energy for the photons reaching the detector, which is then used to calculate the exposure rate or kerma in air. The unfolding and fluency convention parameters are determined by detector calibration using point gamma sources. The method is validated by comparison of the results against the calculated exposure rate at a point of interest for the standards. This method is used for the direct measurement of the exposure rate distribution in function of the energy at the site, in situ measurement technic, leading to rapid results during an emergency situation and also used for indoor measurements. (author)
International Nuclear Information System (INIS)
Monaghan, B.G.; Richards, L.R.
1986-10-01
A final report summarizing the research conducted on geotechnical site assessment methodologies relevant to Land 3/4 sites. Two areas of research have been investigated; in situ stress determination by the hydraulic fracturing method in basic volcanics and sediments and the laboratory determination of hydraulic fracture tensile strength. The analysis and interpretation of the hydraulic fracturing test data from a programme of testing in the Vale of Belvoir is discussed in detail particularly in respect of the effects of pore water pressure and fluid diffusion in the rocks being tested. The regional stress regime of the Vale of Belvoir is discussed with respect to the results of the in situ stress determination. A method for determining the hydraulic fracture tensile strength in the laboratory is described. The results of a series of laboratory tests on rock core are reported. (author)
Novel in-situ lamella fabrication technique for in-situ TEM.
Canavan, Megan; Daly, Dermot; Rummel, Andreas; McCarthy, Eoin K; McAuley, Cathal; Nicolosi, Valeria
2018-03-29
In-situ transmission electron microscopy is rapidly emerging as the premier technique for characterising materials in a dynamic state on the atomic scale. The most important aspect of in-situ studies is specimen preparation. Specimens must be electron transparent and representative of the material in its operational state, amongst others. Here, a novel fabrication technique for the facile preparation of lamellae for in-situ transmission electron microscopy experimentation using focused ion beam milling is developed. This method involves the use of rotating microgrippers during the lift-out procedure, as opposed to the traditional micromanipulator needle and platinum weld. Using rotating grippers, and a unique adhesive substance, lamellae are mounted onto a MEMS device for in-situ TEM annealing experiments. We demonstrate how this technique can be used to avoid platinum deposition as well as minimising damage to the MEMS device during the thinning process. Our technique is both a cost effective and readily implementable alternative to the current generation of preparation methods for in-situ liquid, electrical, mechanical and thermal experimentation within the TEM as well as traditional cross-sectional lamella preparation. Copyright © 2018 Elsevier B.V. All rights reserved.
Krizsan, S J; Jančík, F; Ramin, M; Huhtanen, P
2013-02-01
The objective of the present study was to compare digestion rates (kd) of NDF for different feeds estimated with the in situ method or derived from an automated gas in vitro system. A meta-analysis was conducted to evaluate how in situ derived kd of NDF related to in vivo digestibility of NDF. Furthermore, in vitro true digestibility of the feed samples incubated within filter bags or dispersed in the medium was compared, and kd for insoluble and soluble components of those feeds were estimated. Four different concentrates and 4 forages were used in this study. Two lactating Swedish Red cows fed a diet of 60% grass silage and 40% concentrate on DM basis were used for in situ incubations and for collection of rumen fluid. The feed samples were ground through a 2.0-mm screen before the in situ incubations and a 1.0-mm screen before the in vitro gas incubations. In situ nylon bags were introduced into the rumen for determination of kd of NDF. Additional kinetic data were produced from isolated NDF and intact samples subjected to in vitro incubations in which gas production was recorded for 72 h. Samples were weighed in the bottles or within filter bags (for fiber and in vitro studies) that were placed in the bottles. The interaction between feed and method was significant (P production recordings. The meta-analysis suggested that in situ derived kd of NDF were biased and underestimated in vivo digestibility of NDF. Digestion rates of the intact samples were lower for all feeds, except for the hay, when incubated within the bags compared with dispersed in the medium (P < 0.01). Less OM and NDF were digested for all feeds when incubated within bags than dispersed in the medium (P < 0.01). It is concluded from the in vitro study that microbial activity within the bags is less than in the medium. Significant interactions between method (in situ vs. in vitro) and feed suggest that one or both methods result in biased estimates of digestion kinetics.
International Nuclear Information System (INIS)
Powell, A.W.; Wilkinson, B.A.
1977-01-01
High efficiency air filters are installed in the CEGB's Nuclear Power Stations to prevent discharge of radioactive material to the environment should the ventilation air become contaminated. The Board has determined that these filter units should be tested in-situ to ensure that the stringent design specifications are met. This paper describes the rig work comparing the British Standard method using sodium chloride aerosol with other test methods, such as Dioctyl Phthalate, naturally occurring dust aerosols and artificially produced condensation nuclei. This work led to the development of an in-situ test method using condensation nuclei and a Pollak-Nolan nucleus counter. The advantages and disadvantages of this method over other methods for in-situ testing is discussed. The test method has been used in the South Eastern Region for several years and considerable experience has been gained in its use on a wide variety of filter systems. The method adopted for each system is detailed including the injection, sampling and interpretation of the results obtained. Numerous faults and difficulties have been highlighted from the design, installation and filter changing aspects
Tan, Yongtao; Liu, Ying; Kong, Lingbin; Kang, Long; Ran, Fen
2017-09-01
Nano-Co3O4 decorated with gold nanoparticles is synthesized by a simple method of in-situ reduction of HAuCl4 by sodium citrate for energy storage application, and the effect of gold content in the product on electrochemical performance is investigated in detail. Introducing gold nanoparticles into nano-Co3O4 bulk would contribute to reduce internal resistance of charge transmission. The results show that after in-situ reduction reaction gold nanoparticles imbed uniformly into nano-Co3O4 with irregular nanoparticles. The gold nanoparticles decorated nano-Co3O4 exhibits specific capacitance of 681 F g-1 higher than that of pristine Co3O4 of 368 F g-1. It is interesting that a good cycle life with the specific capacitance retention of 83.1% is obtained after 13000 cycles at 5 A g-1, which recovers to initial specific capacitance value when the test current density is turned to 2 A g-1. In addition, the device of asymmetric supercapacitor, assembled with gold nanoparticles decorated nano-Co3O4 as the positive electrode and activated carbon as the negative electrode, exhibits good energy density of 25 Wh kg-1, which is comparable to the asymmetric device assembled with normal nano-Co3O4, or the symmetric device assembled just with activated carbon.
IN-SITU MEASURING METHOD OF RADON AND THORON DIFFUSION COEFFICIENT IN SOIL
Directory of Open Access Journals (Sweden)
V.S. Yakovleva
2014-06-01
Full Text Available A simple and valid in-situ measurement method of effective diffusion coefficient of radon and thoron in soil and other porous materials was designed. The analysis of numerical investigation of radon and thoron transport in upper layers of soil revealed that thoron flux density from the earth surface does not depend on soil gas advective velocity and varies only with diffusion coefficient changes. This result showed the advantages of thoron using versus radon using in the suggested method. The comparison of the new method with existing ones previously developed. The method could be helpful for solving of problems of radon mass-transport in porous media and gaseous exchange between soil and atmosphere.
Technique for in situ leach simulation of uranium ores
International Nuclear Information System (INIS)
Grant, D.C.; Seidel, D.C.; Nichols, I.L.
1985-01-01
In situ uranium mining offers the advantages of minimal environmental disturbance, low capital and operating costs, and reduced mining development time. It is becoming an increasingly attractive mining method for the recovery of uranium from secondary ore deposits. In order to better understand the process, a laboratory technique was developed and used to study and simulate both the chemical and physical phenomena occurring in ore bodies during in situ leaching. The laboratory simulation technique has been used to determine effects of leaching variables on permeability, uranium recovery, and post-leach aquifer restoration. This report describes the simulation system and testing procedure in sufficient detail to allow the construction of the system, and to perform the desired leaching tests. With construction of such a system, in situ leaching of a given ore using various leach conditions can be evaluated relatively rapidly in the laboratory. Not only could optimum leach conditions be selected for existing ore bodies, but also exploitation of new ore bodies could be accelerated. 8 references, 8 figures, 2 tables
Comparison of in vitro and in situ methods in evaluation of forage digestibility in ruminants.
Krizsan, S J; Nyholm, L; Nousiainen, J; Südekum, K-H; Huhtanen, P
2012-09-01
The objective of this study was to compare the application of different in vitro and in situ methods in empirical and mechanistic predictions of in vivo OM digestibility (OMD) and their associations to near-infrared reflectance spectroscopy spectra for a variety of forages. Apparent in vivo OMD of silages made from alfalfa (n = 2), corn (n = 9), corn stover (n = 2), grass (n = 11), whole crops of wheat and barley (n = 8) and red clover (n = 7), and fresh alfalfa (n = 1), grass hays (n = 5), and wheat straws (n = 5) had previously been determined in sheep. Concentrations of indigestible NDF (iNDF) in all forage samples were determined by a 288-h ruminal in situ incubation. Gas production of isolated forage NDF was measured by in vitro incubations for 72 h. In vitro pepsin-cellulase OM solubility (OMS) of the forages was determined by a 2-step gravimetric digestion method. Samples were also subjected to a 2-step determination of in vitro OMD based on buffered rumen fluid and pepsin. Further, rumen fluid digestible OM was determined from a single 96-h incubation at 38°C. Digestibility of OM from the in situ and the in vitro incubations was calculated according to published empirical equations, which were either forage specific or general (1 equation for all forages) within method. Indigestible NDF was also used in a mechanistic model to predict OMD. Predictions of OMD were evaluated by residual analysis using the GLM procedure in SAS. In vitro OMS in a general prediction equation of OMD did not display a significant forage-type effect on the residuals (observed - predicted OMD; P = 0.10). Predictions of OMD within forage types were consistent between iNDF and the 2-step in vitro method based on rumen fluid. Root mean square error of OMD was least (0.032) when the prediction was based on a general forage equation of OMS. However, regenerating a simple regression for iNDF by omitting alfalfa and wheat straw reduced the root mean square error of OMD to 0
An in-situ measuring method for planar straightness error
Chen, Xi; Fu, Luhua; Yang, Tongyu; Sun, Changku; Wang, Zhong; Zhao, Yan; Liu, Changjie
2018-01-01
According to some current problems in the course of measuring the plane shape error of workpiece, an in-situ measuring method based on laser triangulation is presented in this paper. The method avoids the inefficiency of traditional methods like knife straightedge as well as the time and cost requirements of coordinate measuring machine(CMM). A laser-based measuring head is designed and installed on the spindle of a numerical control(NC) machine. The measuring head moves in the path planning to measure measuring points. The spatial coordinates of the measuring points are obtained by the combination of the laser triangulation displacement sensor and the coordinate system of the NC machine, which could make the indicators of measurement come true. The method to evaluate planar straightness error adopts particle swarm optimization(PSO). To verify the feasibility and accuracy of the measuring method, simulation experiments were implemented with a CMM. Comparing the measurement results of measuring head with the corresponding measured values obtained by composite measuring machine, it is verified that the method can realize high-precise and automatic measurement of the planar straightness error of the workpiece.
Energy Technology Data Exchange (ETDEWEB)
Wagner, M.; Lungerhausen, D. [Erdoel-Erdgas Gommern GmbH (Germany); Murtada, H.; Rosenthal, G. [VEBA OEL AG, Gelsenkirchen (Germany)
1995-12-31
On the basis of different laboratory studies, by which special strains of the type Clostridium tyrobutyricum were found, the application of molasses in-situ method for the enhanced recovery of oil in Romashkino oil field was executed. In an anaerobic, 6%-molasses medium the strains produce about 11,400 mg/l of organic acids (especially butyric acid), 3,200 mg/l ethanol, butanol, etc., and more than 350 ml/g of molasses biogas with a content of 80% C0{sub 2} and 20% H{sub 2}. The metabolics of Clostridium tyrobutyricum depress the growth of SRB, whereas methanogenic bacteria grow in an undiluted fermented molasses medium very well. In this way the dominant final fermentation process is methanogenesis. By laboratory studies with original cores under the conditions of the carbonate reservoir in Bashkir, the recovery of oil increased from 15% after waterflooding to 29% OOIP during the treatment with molasses and bacteria. We developed a new biotechnological method for a self-regulated, automatic continuous culture and constructed a special pilot plant with a high technical standard. The plant produced during the pilot on Romashkino field (September 1992 to August 1994) about 1,000 m{sup 3} of clean inoculum with a content of 3-4 billion cells per ml. This inoculum was injected in slugs together with 15,000 m{sup 3} of molasses medium, first in one, later in five wells. We will demonstrate for two example wells the complex microbiological and chemical changes in the oil, gas, and water phases, and their influences on the recover of oil.
Method for in situ determination cation exchange capacities of subsurface formations
International Nuclear Information System (INIS)
Fertl, W.H.; Welker, D.W.
1980-01-01
A method is disclosed for the in situ examination of each subsurface formation penetrated by a borehole to ascertain the cation exchange capacity of such formations within a geological region. Natural γ ray logging is used to develop signals functionally related to the total γ radiation and to the potassium-40, uranium and thorium energy-band radiations. A first borehole is traversed by a potential γ ray spectrometer to provide selected measurements of natural γ radiation. Core samples are taken from the logged formation and laboratory tests performed to determine the cation exchange capacity thereof. The cation exchange capacities thus are developed then correlated with selected parameters provided by the γ ray spectrometer to establish functional relationships. Cation exchange capacities of formations in subsequent boreholes within the region are then determined in situ by use of the natural γ ray spectrometer and these established relationships. (author)
Directory of Open Access Journals (Sweden)
Mohammad Hassan Fathi Nasri
2016-08-01
Full Text Available Introduction Using of agro-industrial by-products in diet of livestock not only reduces the production costs but also can dwindle the use of human foods in animal nutrition and competition between human and livestock. Thus, proper use of these by-products in animal nutrition and identification of new and low cost feed resources may be one of the priorities in livestock husbandry of our country. Pomegranate seed pulp (PSP is a by-product of the industrial decoction of pomegranate. Iran with annually production of more than 900 tons of pomegranates is one of the most important regions of pomegranate cultivation and PSP produced from pomegranate processing factories may be extensively used in animal nutrition. Digestibility determination of feeds is one of the most effective ways to evaluate their nutritional value. In addition, there is a strong relationship between feeds digestibility and performance of animal. There are in vivo, in vitro and in situ methods to determine the digestibility of feeds. Although in vivo methods are reference for digestibility values of feeds and are of high precision but they are usually expensive and time consuming. In addition, these methods do not provide any information related to ruminal degradability kinetic of nutrients. The aim of this study was comparison of two methods of ruminal degradability determination methods namely, in situ (nylon bag and in vitro (Daisy incubator, using dried and ensiled pomegranate seed pulp (PSP. Materials and Methods The PSP used in this study was prepared from Anaryan Co. in Ferdows, Iran. Decocted pomegranate was a mixture of Yazd varieties which were harvested at early autumn of 1389. Two types of PSP as dried and ensiled were used to compare the methods of ruminal digestibility determination, pervasively. The PSP silage was prepared by ensiling of wet PSP (containing 475 g/kg DM in 3 kg bins (4 replicatesand with density of 650 kg wet PSP per cubic meter for 60 days and dried
Rozin, A. G.; Clark, M. W.
2013-12-01
Assessing the impact of nutrient concentrations on aquatic ecosystems requires an in depth understanding of dynamic biogeochemical cycles that are often a challenge to monitor at the high spatial and temporal resolution necessary to understand these complex processes. Traditional sampling approaches involving discrete samples and laboratory analyses can be constrained by analytical costs, field time, and logistical details that can fail to accurately capture both spatial and temporal changes. Optical in situ instruments may provide the opportunity to continuously monitor a variety of water quality parameters at a high spatial or temporal resolution. This work explores the suitability of a Submersible Ultraviolet Nitrate Analyzer (SUNA), produced by Satlantic, to accurately assess in situ nitrate concentration in several freshwater systems in north Florida. The SUNA was deployed to measure nitrate at five different water bodies selected to represent a range of watershed land uses and water chemistry in the region. In situ nitrate measurements were compared to standard laboratory methods to evaluate the effectiveness of the SUNA's operation. Other optical sensors were used to measure the spectral properties of absorbance, fluorescence, and turbidity (scatter) in the same Florida water bodies. Data from these additional sensors were collected to quantify possible interferences that may affect SUNA performance. In addition, data from the SUNA and other sensors are being used to infer information about the quality and quantity of aqueous constituents besides nitrate. A better understanding of the capabilities and possible limitations of these relatively new analytical instruments will allow researchers to more effectively investigate biogeochemical processes and nutrient transport and enhance decision-making to protect our water bodies.
Human activity and rest in situ.
Roenneberg, Till; Keller, Lena K; Fischer, Dorothee; Matera, Joana L; Vetter, Céline; Winnebeck, Eva C
2015-01-01
Our lives are structured by the daily alternation of activity and rest, of wake and sleep. Despite significant advances in circadian and sleep research, we still lack answers to many of the most fundamental questions about this conspicuous behavioral pattern. We strongly believe that investigating this pattern in entrained conditions, real-life and daily contexts-in situ-will help the field to elucidate some of these central questions. Here, we present two common approaches for in situ investigation of human activity and rest: the Munich ChronoType Questionnaire (MCTQ) and actimetry. In the first half of this chapter, we provide detailed instructions on how to use and interpret the MCTQ. In addition, we give an overview of the main insights gained with this instrument over the past 10 years, including some new findings on the interaction of light and age on sleep timing. In the second half of this chapter, we introduce the reader to the method of actimetry and share our experience in basic analysis techniques, including visualization, smoothing, and cosine model fitting of in situ recorded data. Additionally, we describe our new approach to automatically detect sleep from activity recordings. Our vision is that the broad use of such easy techniques in real-life settings combined with automated analyses will lead to the creation of large databases. The resulting power of big numbers will promote our understanding of such fundamental biological phenomena as sleep. © 2015 Elsevier Inc. All rights reserved.
In situ vitrification - A potential remedial action technique for hazardous wastes
International Nuclear Information System (INIS)
Fitzpatrick, V.F.; Buelt, J.L.; Oma, K.H.; Timmerman, C.L.
1984-01-01
In situ vitrification (ISV) is an innovative technology being developed as a potential method for stabilizing transuranic (TRU) contaminated wastes in place. Although the process is being developed for TRU contaminated wastes, it is envisioned that the process could also be applied to hazardous chemical wastes. In situ vitrification (ISV) is the conversion of contaminated soil into a durable glass and crystalline wastes form through melting by joule heating. The technology for in situ vitrification is based upon electric melter technology developed at the Pacific Northwest Laboratory (PNL) for the immobilization of high-level nuclear waste. In situ vitrification was initially tested by researchers at PNL in August, 1980 (U.S. Patent 4,376,598). Since then, ISV has grown from a concept to an emerging technology through a series of 21 engineering-scale (laboratory) tests and 7 pilot-scale (field) tests. A large-scale system is currently being fabricated for testing. The program has been sponsored by the U.S. Department of Energy's (DOE) Richland Operations Office for potential application to Hanford TRU contaminated soil sites. A more detailed description outlining the power system design and the off-gas treatment system follows
Evaluation of Methods for In-Situ Calibration of Field-Deployable Microphone Phased Arrays
Humphreys, William M.; Lockard, David P.; Khorrami, Mehdi R.; Culliton, William G.; McSwain, Robert G.
2017-01-01
Current field-deployable microphone phased arrays for aeroacoustic flight testing require the placement of hundreds of individual sensors over a large area. Depending on the duration of the test campaign, the microphones may be required to stay deployed at the testing site for weeks or even months. This presents a challenge in regards to tracking the response (i.e., sensitivity) of the individual sensors as a function of time in order to evaluate the health of the array. To address this challenge, two different methods for in-situ tracking of microphone responses are described. The first relies on the use of an aerial sound source attached as a payload on a hovering small Unmanned Aerial System (sUAS) vehicle. The second relies on the use of individually excited ground-based sound sources strategically placed throughout the array pattern. Testing of the two methods was performed in microphone array deployments conducted at Fort A.P. Hill in 2015 and at Edwards Air Force Base in 2016. The results indicate that the drift in individual sensor responses can be tracked reasonably well using both methods. Thus, in-situ response tracking methods are useful as a diagnostic tool for monitoring the health of a phased array during long duration deployments.
Kunju, Lakshmi P; Carskadon, Shannon; Siddiqui, Javed; Tomlins, Scott A; Chinnaiyan, Arul M; Palanisamy, Nallasivam
2014-09-01
The genetic basis of 50% to 60% of prostate cancer (PCa) is attributable to rearrangements in E26 transformation-specific (ETS) (ERG, ETV1, ETV4, and ETV5), BRAF, and RAF1 genes and overexpression of SPINK1. The development and validation of reliable detection methods are warranted to classify various molecular subtypes of PCa for diagnostic and prognostic purposes. ETS gene rearrangements are typically detected by fluorescence in situ hybridization and reverse-transcription polymerase chain reaction methods. Recently, monoclonal antibodies against ERG have been developed that detect the truncated ERG protein in immunohistochemical assays where staining levels are strongly correlated with ERG rearrangement status by fluorescence in situ hybridization. However, specific antibodies for ETV1, ETV4, and ETV5 are unavailable, challenging their clinical use. We developed a novel RNA in situ hybridization-based assay for the in situ detection of ETV1, ETV4, and ETV5 in formalin-fixed paraffin-embedded tissues from prostate needle biopsies, prostatectomy, and metastatic PCa specimens using RNA probes. Further, with combined RNA in situ hybridization and immunohistochemistry we identified a rare subset of PCa with dual ETS gene rearrangements in collisions of independent tumor foci. The high specificity and sensitivity of RNA in situ hybridization provides an alternate method enabling bright-field in situ detection of ETS gene aberrations in routine clinically available PCa specimens.
Energy Technology Data Exchange (ETDEWEB)
Conti, Claudio de Carvalho
1995-07-15
This work tries to establish a methodology for germanium detectors calibration, normally used for in situ gamma ray spectrometry, for determining the environmental exposure rate in function of the energy of the incident photons. For this purpose a computer code has been developed, based on the stripping method, for the computational spectra analysis to calculate the contribution of the partial absorption of the gamma rays (Compton effect) in the active and nonactive parts of the detector. The resulting total absorption spectrum is then converted to fluence distribution in function of the energy for the photons reaching the detector, which is then used to calculate the exposure rate or kerma in air. The unfolding and fluency convention parameters are determined by detector calibration using point gamma sources. The method is validated by comparison of the results against the calculated exposure rate at a point of interest for the standards. This method is used for the direct measurement of the exposure rate distribution in function of the energy at the site, in situ measurement technic, leading to rapid results during an emergency situation and also used for indoor measurements. (author)
The SENSEI Generic In Situ Interface
Energy Technology Data Exchange (ETDEWEB)
Ayachit, Utkarsh [Kitware, Inc., Clifton Park, NY (United States); Whitlock, Brad [Intelligent Light, Rutherford, NJ (United States); Wolf, Matthew [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Loring, Burlen [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Geveci, Berk [Kitware, Inc., Clifton Park, NY (United States); Lonie, David [Kitware, Inc., Clifton Park, NY (United States); Bethel, E. Wes [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
2017-04-11
The SENSEI generic in situ interface is an API that promotes code portability and reusability. From the simulation view, a developer can instrument their code with the SENSEI API and then make make use of any number of in situ infrastructures. From the method view, a developer can write an in situ method using the SENSEI API, then expect it to run in any number of in situ infrastructures, or be invoked directly from a simulation code, with little or no modification. This paper presents the design principles underlying the SENSEI generic interface, along with some simplified coding examples.
Calibration of HPGe detector for in situ measurements of 137Cs in soil by 'peak to valley' method
International Nuclear Information System (INIS)
Fueloep, M.
2000-01-01
The contamination of soil with gamma-ray emitters can be measured in two ways: soil sampling method and in situ spectrometry of the ambient gamma-ray radiation. The conventional soil sampling method has two disadvantages: samples may not be representative for a large areas and determination of the depth distribution of radionuclide requires the measurement of several samples taken from different depths. In situ measurement of a radionuclide activity in soil is more sensitive and provides more representative data than data obtained by soil sample collection and subsequent laboratory analysis. In emergency situations time to assess the contamination is critical. For rapid assessment of the deposited activity direct measurement of ambient gamma-ray radiation are used. In order to obtain accurate measurements of radionuclides in the soil, the detector should be placed on relatively even and open terrain. It is our customary practice to place the detector 1 m above the soil surface. At this height, a tripod-mounted detector can be handled easily and still provide a radius of view for gamma emitting sources out to about 10 m. The 'field of view' actually varies, being somewhat larger for higher sources. Depending upon source energy, the detector effectively sees down to a depth of 15-30 cm. Commonly used method for field gamma spectrometry is method by Beck (1). The most important disadvantages of in situ spectrometry by Beck are that the accuracy of the analysis depends on a separate knowledge of the radioactivity distribution with soil depth. This information can be obtained by calculations using data from in situ measurements and energy dependence of absorption and scattering of photons in soil and track length distribution of photons in soil (2). A method of in situ measurements of 137 Cs in soil where radionuclide distribution in soil profile is calculated by unfolding of detector responses in the full energy peak net area at 0.662 MeV and in the valley under the
Monitoring dynamic electrochemical processes with in situ ptychography
Kourousias, George; Bozzini, Benedetto; Jones, Michael W. M.; Van Riessen, Grant A.; Dal Zilio, Simone; Billè, Fulvio; Kiskinova, Maya; Gianoncelli, Alessandra
2018-03-01
The present work reports novel soft X-ray Fresnel CDI ptychography results, demonstrating the potential of this method for dynamic in situ studies. Specifically, in situ ptychography experiments explored the electrochemical fabrication of Co-doped Mn-oxide/polypyrrole nanocomposites for sustainable and cost-effective fuel-cell air-electrodes. Oxygen-reduction catalysts based on Mn-oxides exhibit relatively high activity, but poor durability: doping with Co has been shown to improve both reduction rate and stability. In this study, we examine the chemical state distribution of the catalytically crucial Co dopant to elucidate details of the Co dopant incorporation into the Mn/polymer matrix. The measurements were performed using a custom-made three-electrode thin-layer microcell, developed at the TwinMic beamline of Elettra Synchrotron during a series of experiments that were continued at the SXRI beamline of the Australian Synchrotron. Our time-resolved ptychography-based investigation was carried out in situ after two representative growth steps, controlled by electrochemical bias. In addition to the observation of morphological changes, we retrieved the spectroscopic information, provided by multiple ptychographic energy scans across Co L3-edge, shedding light on the doping mechanism and demonstrating a general approach for the molecular-level investigation complex multimaterial electrodeposition processes.
DEFF Research Database (Denmark)
Yue, Zhao; Grivel, Jean-Claude; He, Dong
2012-01-01
In situ synchrotron x-ray diffraction is used to study the phase and texture formation of ceria based films and superconductor films deposited by the chemical solution method on technical substrates. Combined analysis using in situ synchrotron x-ray diffraction, thermogravimetry/differential ther......In situ synchrotron x-ray diffraction is used to study the phase and texture formation of ceria based films and superconductor films deposited by the chemical solution method on technical substrates. Combined analysis using in situ synchrotron x-ray diffraction, thermogravimetry...
In situ anisotropic parameter determination using refraction seismic and VSP methods
Energy Technology Data Exchange (ETDEWEB)
Leslie, J.M.; Lawton, D.C. (Calgary Univ., AB (Canada))
1999-01-01
A prime concern in the time-to-depth conversion of reflection seismic data is seismic anisotropy, because it can produce velocity anomalies in seismic data that mimic the structural plays of interest to the petroleum prospector in both size and shape. Ongoing techniques of time-to-depth conversion of P-wave seismic data do not handle the travel time and velocity distortions caused by seismic anisotropy, particularly in areas of complex geologic structures. To address this problem, the first step is to know which rock units are anisotropic and measure their anisotropic parameters. Laboratory means are available, but there are problems with these mainly with shales because of their fissile nature. In situ measurements are preferable because they yield a more robust value, and at the University of Calgary such measurements were undertaken using refraction seismic and vertical seismic profiling (VSP) methods. Results indicate that the two Thomsen anisotropic parameters of interest can be determined from the VSP experiment, but these values are slightly less than those obtained using the refraction technique. This may be because of the sensitivity of the shot statics which arises from the direct travel time measurement of the technique. The experiment yields another method to measure velocity anisotropy, in situ, where steeply dipping strata outcrop, which allows for the accurate measurement of the anisotropic parameters for use in depth migration routines. 4 refs.
In situ anisotropic parameter determination using refraction seismic and VSP methods
Energy Technology Data Exchange (ETDEWEB)
Leslie, J.M.; Lawton, D.C. [Calgary Univ., AB (Canada)
1999-11-01
A prime concern in the time-to-depth conversion of reflection seismic data is seismic anisotropy, because it can produce velocity anomalies in seismic data that mimic the structural plays of interest to the petroleum prospector in both size and shape. Ongoing techniques of time-to-depth conversion of P-wave seismic data do not handle the travel time and velocity distortions caused by seismic anisotropy, particularly in areas of complex geologic structures. To address this problem, the first step is to know which rock units are anisotropic and measure their anisotropic parameters. Laboratory means are available, but there are problems with these mainly with shales because of their fissile nature. In situ measurements are preferable because they yield a more robust value, and at the University of Calgary such measurements were undertaken using refraction seismic and vertical seismic profiling (VSP) methods. Results indicate that the two Thomsen anisotropic parameters of interest can be determined from the VSP experiment, but these values are slightly less than those obtained using the refraction technique. This may be because of the sensitivity of the shot statics which arises from the direct travel time measurement of the technique. The experiment yields another method to measure velocity anisotropy, in situ, where steeply dipping strata outcrop, which allows for the accurate measurement of the anisotropic parameters for use in depth migration routines. 4 refs.
Design, installation and in-situ performance study of ultrasonic continuous level meter
International Nuclear Information System (INIS)
Surya Prakash, G.; Somayaji, K.M.
1995-01-01
The report discusses the design and the development of a continuous liquid level meter based on ultrasonic pulse-echo technique. A review of various methods for level measurement along with the details of pulse-echo technique are presented. Also discussed is the development of discrete level detectors based on ultrasonic techniques. Lastly, the procedures adopted for in-situ installation of these level meters in FBTR and RML are given along with the field performance study. Some of the typical merits and limitations of the method and the surface preparation procedure are also highlighted. (author) (author)
A practical method for in-situ thickness determination using energy distribution of beta particles
International Nuclear Information System (INIS)
Yalcin, S.; Gurler, O.; Gundogdu, O.; Bradley, D.A.
2012-01-01
This paper discusses a method to determine the thickness of an absorber using the energy distribution of beta particles. An empirical relationship was obtained between the absorber thickness and the energy distribution of beta particles transmitted through. The thickness of a polyethylene radioactive source cover was determined by exploiting this relationship, which has largely been left unexploited allowing us to determine the in-situ cover thickness of beta sources in a fast, cheap and non-destructive way. - Highlights: ► A practical and in-situ unknown cover thickness determination ► Cheap and readily available compared to other techniques. ► Beta energy spectrum.
A practical method for in-situ thickness determination using energy distribution of beta particles
Energy Technology Data Exchange (ETDEWEB)
Yalcin, S., E-mail: syalcin@kastamonu.edu.tr [Kastamonu University, Education Faculty, 37200 Kastamonu (Turkey); Gurler, O. [Physics Department, Faculty of Arts and Sciences, Uludag University, Gorukle Campus, 16059 Bursa (Turkey); Gundogdu, O. [Kocaeli University, Umuttepe Campus, 41380 Kocaeli (Turkey); Bradley, D.A. [CNRP, Department of Physics, Faculty of Engineering and Physical Sciences, University of Surrey, Guildford GU2 7XH (United Kingdom)
2012-01-15
This paper discusses a method to determine the thickness of an absorber using the energy distribution of beta particles. An empirical relationship was obtained between the absorber thickness and the energy distribution of beta particles transmitted through. The thickness of a polyethylene radioactive source cover was determined by exploiting this relationship, which has largely been left unexploited allowing us to determine the in-situ cover thickness of beta sources in a fast, cheap and non-destructive way. - Highlights: Black-Right-Pointing-Pointer A practical and in-situ unknown cover thickness determination Black-Right-Pointing-Pointer Cheap and readily available compared to other techniques. Black-Right-Pointing-Pointer Beta energy spectrum.
International Nuclear Information System (INIS)
Dotson, B.J.
1986-01-01
This invention provides a method for improving the recovery of mineral values from ore bodies subjected to in-situ leaching by controlling the flow behaviour of the leaching solution. In particular, the invention relates to an in-situ leaching operation employing a foam for mobility control of the leaching solution. A foam bank is either introduced into the ore bed or developed in-situ in the ore bed. The foam then becomes a diverting agent forcing the leaching fluid through the previously non-contacted regions of the deposit
Estimation of In Situ Stresses with Hydro-Fracturing Tests and a Statistical Method
Lee, Hikweon; Ong, See Hong
2018-03-01
At great depths, where borehole-based field stress measurements such as hydraulic fracturing are challenging due to difficult downhole conditions or prohibitive costs, in situ stresses can be indirectly estimated using wellbore failures such as borehole breakouts and/or drilling-induced tensile failures detected by an image log. As part of such efforts, a statistical method has been developed in which borehole breakouts detected on an image log are used for this purpose (Song et al. in Proceedings on the 7th international symposium on in situ rock stress, 2016; Song and Chang in J Geophys Res Solid Earth 122:4033-4052, 2017). The method employs a grid-searching algorithm in which the least and maximum horizontal principal stresses ( S h and S H) are varied, and the corresponding simulated depth-related breakout width distribution as a function of the breakout angle ( θ B = 90° - half of breakout width) is compared to that observed along the borehole to determine a set of S h and S H having the lowest misfit between them. An important advantage of the method is that S h and S H can be estimated simultaneously in vertical wells. To validate the statistical approach, the method is applied to a vertical hole where a set of field hydraulic fracturing tests have been carried out. The stress estimations using the proposed method were found to be in good agreement with the results interpreted from the hydraulic fracturing test measurements.
In-situ testing methods using radioactive tracers
International Nuclear Information System (INIS)
Sauzay, G.
1976-01-01
Some typical applications of tracer techniques in hydrology are presented: study of the extraction of sands and gravels in a estuary; in-situ study of the transport of sediments by the swell at a depth ranging from 8 to 22m; study of the transport of sands on the site Bonne Anse - Saint Palais [fr
Vendelbo, S B; Kooyman, P J; Creemer, J F; Morana, B; Mele, L; Dona, P; Nelissen, B J; Helveg, S
2013-10-01
In situ high-resolution transmission electron microscopy (TEM) of solids under reactive gas conditions can be facilitated by microelectromechanical system devices called nanoreactors. These nanoreactors are windowed cells containing nanoliter volumes of gas at ambient pressures and elevated temperatures. However, due to the high spatial confinement of the reaction environment, traditional methods for measuring process parameters, such as the local temperature, are difficult to apply. To address this issue, we devise an electron energy loss spectroscopy (EELS) method that probes the local temperature of the reaction volume under inspection by the electron beam. The local gas density, as measured using quantitative EELS, is combined with the inherent relation between gas density and temperature, as described by the ideal gas law, to obtain the local temperature. Using this method we determined the temperature gradient in a nanoreactor in situ, while the average, global temperature was monitored by a traditional measurement of the electrical resistivity of the heater. The local gas temperatures had a maximum of 56 °C deviation from the global heater values under the applied conditions. The local temperatures, obtained with the proposed method, are in good agreement with predictions from an analytical model. Copyright © 2013 Elsevier B.V. All rights reserved.
Turick, Charles E.; Apel, William W.
1997-10-28
A method of reducing the concentration of Cr(VI) in a liquid aqueous residue comprises the steps of providing anaerobic Cr(VI) reducing bacteria, mixing the liquid aqueous residue with a nutrient medium to form a mixture, and contacting the mixture with the anaerobic Cr(VI) reducing bacteria such that Cr(VI) is reduced to Cr(III). The anaerobic Cr(VI) reducing bacteria appear to be ubiquitous in soil and can be selected by collecting a soil sample, diluting the soil sample with a sterile diluent to form a diluted sample, mixing the diluted sample with an effective amount of a nutrient medium and an effective amount of Cr(VI) to form a mixture, and incubating the mixture in the substantial absence of oxygen such that growth of Cr(VI) sensitive microorganisms is inhibited and growth of the anaerobic Cr(VI) reducing bacteria is stimulated. A method of in situ bioremediation of Cr(VI) contaminated soil and/or groundwater is also disclosed.
International Nuclear Information System (INIS)
Ohlsson, Y.; Neretnieks, I.
1998-01-01
Traditional laboratory diffusion experiments in rock material are time consuming, and quite small samples are generally used. Electrical conductivity measurements, on the other hand, provide a fast means for examining transport properties in rock and allow measurements on larger samples as well. Laboratory measurements using electrical conductivity give results that compare well to those from traditional diffusion experiments. The measurement of the electrical resistivity in the rock surrounding a borehole is a standard method for the detection of water conducting fractures. If these data could be correlated to matrix diffusion properties, in-situ diffusion data from large areas could be obtained. This would be valuable because it would make it possible to obtain data very early in future investigations of potentially suitable sites for a repository. This study compares laboratory electrical conductivity measurements with in-situ resistivity measurements from a borehole at Aespoe. The laboratory samples consist mainly of Aespoe diorite and fine-grained granite and the rock surrounding the borehole of Aespoe diorite, Smaaland granite and fine-grained granite. The comparison shows good agreement between laboratory measurements and in-situ data
Experimental methods for in situ studies of morphology development during flow
DEFF Research Database (Denmark)
Lyngaae-Jørgensen, Jørgen
1996-01-01
A short overview of in situ methods is given. Multiple laminates of thin films is believed to represent unstable transition structures in many processing operations. A hypotesis for the break up is that first thin cylinders are formed . At a later stage these cylinders break up to spheres...... breakup mechanism involving creation of parallel layers of thin, filmlike formations parallel with the original film is observed. These layers then break up to a final state encompasing nearly spherical drops spread over a thickness much larger (by orders of magnitude) than the original film thickness....
Directory of Open Access Journals (Sweden)
Dajian Huang
2017-11-01
Full Text Available In order to sustain rapid expansion in the field of biocomposites, it is necessary to develop novel fillers that are biodegradable, and easy to disperse and obtain. In this work, gliadin particles (GPs fabricated through an in situ method have been reported as fillers for creating chitosan (CS-based biocomposite films. In general, the particles tend to agglomerate in the polymer matrix at high loading (approximately >10% in the biopolymer/particles composites prepared by the traditional solution-blending method. However, the micrographs of biocomposites confirmed that the GPs are well dispersed in the CS matrix in all CS/GPs composites even at a high loading of 30% in this study. It was found that the GPs could improve the mechanical properties of the biocomposites. In addition, the results of moisture uptake and solubility in water of biocomposites showed that water resistance of biocomposites was enhanced by the introduction of GPs. These results suggested that GPs fabricated through an in situ method could be a good candidate for use in biopolymer-based composites.
DEFF Research Database (Denmark)
Bjerregaard, Anders Thais; Slot, Susanne; Paltved, Charlotte
2015-01-01
, and organisational characteristic. Therefore, it might fail to fully mimic real clinical team processes. Though research on in situ simulation in healthcare is in its infancy, literature is abundant on patient safety and team training1. Patient safety reporting systems that identify risks to patients can improve......Introduction: In situ simulation offers on-site training to healthcare professionals. It refers to a training strategy where simulation technology is integrated into the clinical encounter. Training in the simulation laboratory does not easily tap into situational resources, e.g. individual, team...... patient safety if coupled with training and organisational support. This study explored the use of critical incidents and adverse events reports for in situ simulation and short-term observations were used to create learning objectives and training scenarios. Method: This study used an interventional case...
Directory of Open Access Journals (Sweden)
Yuting Tang
2016-11-01
Full Text Available Microalgae are a valuable source of lipid feedstocks for biodiesel and valuable omega-3 fatty acids. Nannochloropsis gaditana has emerged as a promising producer of eicosapentaenoic acid (EPA due to its fast growth rate and high EPA content. In the present study, the fatty acid profile of Nannochloropsis gaditana was found to be naturally high in EPA and devoid of docosahexaenoic acid (DHA, thereby providing an opportunity to maximize the efficacy of EPA production. Using an optimized one-step in situ transesterification method (methanol:biomass = 90 mL/g; HCl 5% by vol.; 70 °C; 1.5 h, the maximum fatty acid methyl ester (FAME yield of Nannochloropsis gaditana cultivated under rich condition was quantified as 10.04% ± 0.08% by weight with EPA-yields as high as 4.02% ± 0.17% based on dry biomass. The total FAME and EPA yields were 1.58- and 1.23-fold higher separately than that obtained using conventional two-step method (solvent system: methanol and chloroform. This one-step in situ method provides a fast and simple method to measure fatty acid methyl ester (FAME yields and could serve as a promising method to generate eicosapentaenoic acid methyl ester from microalgae.
Directory of Open Access Journals (Sweden)
Qingchuan Guo
2014-07-01
Full Text Available This article reports about nanocomposites, which refractive index is tuned by adding TiO2 nanoparticles. We compare in situ/ex situ preparation of nanocomposites. Preparation procedure is described, properties of nanocomposites are compared, and especially we examine the applicability of two-photon polymerization (2PP of synthesized nanocomposites. All prepared samples exhibit suitable optical transparency at specific laser wavelengths. Three-dimensional structures were generated by means of two-photon polymerization effect induced by a femtosecond laser.
International Nuclear Information System (INIS)
Liu, Guanghua; Li, Jiangtao; Yang, Zengchao; Guo, Shibin; Chen, Yixiang
2013-01-01
A new method of high-gravity combustion synthesis and in situ melt infiltration is reported for preparing cemented carbides, where hot nickel melt is in situ synthesized from a highly exothermic combustion reaction and then infiltrated into tungsten carbide powder compacts. The as-prepared sample showed a homogeneous microstructure, and its relative density, hardness and flexural strength were 94.4%, 84 HRA and 1.49 GPa, respectively. Compared with conventional powder metallurgy approaches, high-gravity combustion synthesis offers a fast and furnace-free way to produce cemented carbides
A new in-situ method to determine the apparent gas diffusion coefficient of soils
Laemmel, Thomas; Paulus, Sinikka; Schack-Kirchner, Helmer; Maier, Martin
2015-04-01
Soil aeration is an important factor for the biological activity in the soil and soil respiration. Generally, gas exchange between soil and atmosphere is assumed to be governed by diffusion and Fick's Law is used to describe the fluxes in the soil. The "apparent soil gas diffusion coefficient" represents the proportional factor between the flux and the gas concentration gradient in the soil and reflects the ability of the soil to "transport passively" gases through the soil. One common way to determine this coefficient is to take core samples in the field and determine it in the lab. Unfortunately this method is destructive and needs laborious field work and can only reflect a small fraction of the whole soil. As a consequence insecurity about the resulting effective diffusivity on the profile scale must remain. We developed a new in-situ method using new gas sampling device, tracer gas and inverse soil gas modelling. The gas sampling device contains several sampling depths and can be easily installed into vertical holes of an auger, which allows for fast installation of the system. At the lower end of the device inert tracer gas is injected continuously. The tracer gas diffuses into the surrounding soil. The resulting distribution of the tracer gas concentrations is used to deduce the diffusivity profile of the soil. For Finite Element Modeling of the gas sampling device/soil system the program COMSOL is used. We will present the results of a field campaign comparing the new in-situ method with lab measurements on soil cores. The new sampling pole has several interesting advantages: it can be used in-situ and over a long time; so it allows following modifications of diffusion coefficients in interaction with rain but also vegetation cycle and wind.
Energy Technology Data Exchange (ETDEWEB)
Koca, Atif [Department of Chemical Engineering, Faculty of Engineering, Marmara University, Goeztepe, 34722 Istanbul (Turkey)], E-mail: akoca@eng.marmara.edu.tr; Bayar, Serife; Dincer, Hatice A. [Department of Chemistry, Technical University of Istanbul, Maslak, 34469 Istanbul (Turkey); Gonca, Erguen [Department of Chemistry, Fatih University, TR34500 B.Cekmece, Istanbul (Turkey)
2009-04-01
In this work, electrochemical, and in-situ spectroelectrochemical characterization of the metallophthalocyanines bearing tetra-(1,1-(dicarbethoxy)-2-(2-methylbenzyl))-ethyl 3,10,17,24-tetra chloro groups were performed. Voltammetric and in-situ spectroelectrochemical measurements show that while cobalt phthalocyanine complex gives both metal-based and ring-based redox processes, zinc and copper phthalocyanines show only ring-based reduction and oxidation processes. The redox processes are generally diffusion-controlled, reversible and one-electron transfer processes. Differently lead phthalocyanine demetallized during second oxidation reaction while it was stable during reduction processes. An in-situ electrocolorimetric method, based on the 1931 CIE (Commission Internationale de l'Eclairage) system of colorimetry, has been applied to investigate the color of the electro-generated anionic and cationic forms of the complexes for the first time in this study.
International Nuclear Information System (INIS)
Gancs, L.; Nemeth, Z.; Horanyi, G.
2002-01-01
Radiotracer methods, particularly the radiotracer thin foil method, provide unique possibility of in situ monitoring of chromate adsorption on powdered adsorbents. Two different versions of the thin foil method can be distinguished. In the direct method, the species to be studied is labelled and the radiation measured gives direct information on the adsorption of this species. In the indirect method, a different labelled indicator species is added to the system and the adsorption of this species is followed and the adsorption of the species to be studied is determined based on analysis of the competitive adsorption processes. Both methods were used in the present study. In the in situ methods, the radiation measured consists of two main parts, one coming from the solution background, the other originating from the adsorption layer. In the case of the thin foil method using isotopes emitting soft β - radiation or low energy X-ray the solution background is governed and minimised by self-absorption of the radiation. In the direct study we applied an experimental methodology based on the energy selective measurement of the characteristic K α,β X-radiation emitted by the 51 Cr-labelled chromate species, whereas 35 S-labelled sulphate ions were used as the indicator species in the indirect study. (P.A.)
Absorption mechanism of three curcumin constituents through in situ intestinal perfusion method
Directory of Open Access Journals (Sweden)
Y.-H. Wang
2017-09-01
Full Text Available This study aimed to investigate the absorption mechanism of three curcumin constituents in rat small intestines. Self-emulsification was used to solubilize the three curcumin constituents, and the rat in situ intestinal perfusion method was used to study factors on drug absorption, including drug mass concentration, absorption site, and the different types and concentrations of absorption inhibitors. Within the scope of experimental concentrations, three curcumin constituents were absorbed in rat small intestines through the active transport mechanism.
Four Models of In Situ Simulation
DEFF Research Database (Denmark)
Musaeus, Peter; Krogh, Kristian; Paltved, Charlotte
2014-01-01
Introduction In situ simulation is characterized by being situated in the clinical environment as opposed to the simulation laboratory. But in situ simulation bears a family resemblance to other types of on the job training. We explore a typology of in situ simulation and suggest that there are f......Introduction In situ simulation is characterized by being situated in the clinical environment as opposed to the simulation laboratory. But in situ simulation bears a family resemblance to other types of on the job training. We explore a typology of in situ simulation and suggest...... that there are four fruitful approaches to in situ simulation: (1) In situ simulation informed by reported critical incidents and adverse events from emergency departments (ED) in which team training is about to be conducted to write scenarios. (2) In situ simulation through ethnographic studies at the ED. (3) Using...... the following processes: Transition processes, Action processes and Interpersonal processes. Design and purpose This abstract suggests four approaches to in situ simulation. A pilot study will evaluate the different approaches in two emergency departments in the Central Region of Denmark. Methods The typology...
Sinigaglia, Chiara; Thiel, Daniel; Hejnol, Andreas; Houliston, Evelyn; Leclère, Lucas
2018-02-01
In situ hybridization is a widely employed technique allowing spatial visualization of gene expression in fixed specimens. It has greatly advanced our understanding of biological processes, including developmental regulation. In situ protocols are today routinely followed in numerous laboratories, and although details might change, they all include a hybridization step, where specific antisense RNA or DNA probes anneal to the target nucleic acid sequence. This step is generally carried out at high temperatures and in a denaturing solution, called hybridization buffer, commonly containing 50% (v/v) formamide - a hazardous chemical. When applied to the soft-bodied hydrozoan medusa Clytia hemisphaerica, we found that this traditional hybridization approach was not fully satisfactory, causing extensive deterioration of morphology and tissue texture which compromised our observation and interpretation of results. We thus tested alternative solutions for in situ detection of gene expression and, inspired by optimized protocols for Northern and Southern blot analysis, we substituted the 50% formamide with an equal volume of 8M urea solution in the hybridization buffer. Our new protocol not only yielded better morphologies and tissue consistency, but also notably improved the resolution of the signal, allowing more precise localization of gene expression and reducing aspecific staining associated with problematic areas. Given the improved results and reduced manipulation risks, we tested the urea protocol on other metazoans, two brachiopod species (Novocrania anomala and Terebratalia transversa) and the priapulid worm Priapulus caudatus, obtaining a similar reduction of aspecific probe binding. Overall, substitution of formamide by urea during in situ hybridization offers a safer alternative, potentially of widespread use in research, medical and teaching contexts. We encourage other workers to test this approach on their study organisms, and hope that they will also
Demonstration of in situ-constructed horizontal soil containment barrier at Fernald
International Nuclear Information System (INIS)
Pettit, P.J.; Ridenour, D.; Walker, J.; Saugier, K.
1994-01-01
A new design of jet grouting tool that can be guided by horizontal well casings and that operates in the horizontal plane has been used for the in situ placement of grout and construction of a prototype horizontal barrier that is free of windows. Jet grouting techniques have been advanced to permit construction of horizontal barriers underneath contaminated soil without having to excavate or disturb the waste. The paper describes progress on the Fernald Environmental Restoration Management Corporation (FERMCO) In Situ Land Containment Project which is sponsored by the US Department of Energy's (DOE) Office of Technology Development (OTD) for DOE's Fernald Environmental Management Project (FEMP). The Fernald project is to demonstrate a novel, enabling technology for the controlled underground placement of horizontal panels of grout, and the joining of adjacent panels to construct practical, extensive barriers. Construction strategy, equipment mechanics and operating details of this new method are described
Scalable Analysis Methods and In Situ Infrastructure for Extreme Scale Knowledge Discovery
Energy Technology Data Exchange (ETDEWEB)
Bethel, Wes [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
2016-07-24
The primary challenge motivating this team’s work is the widening gap between the ability to compute information and to store it for subsequent analysis. This gap adversely impacts science code teams, who are able to perform analysis only on a small fraction of the data they compute, resulting in the very real likelihood of lost or missed science, when results are computed but not analyzed. Our approach is to perform as much analysis or visualization processing on data while it is still resident in memory, an approach that is known as in situ processing. The idea in situ processing was not new at the time of the start of this effort in 2014, but efforts in that space were largely ad hoc, and there was no concerted effort within the research community that aimed to foster production-quality software tools suitable for use by DOE science projects. In large, our objective was produce and enable use of production-quality in situ methods and infrastructure, at scale, on DOE HPC facilities, though we expected to have impact beyond DOE due to the widespread nature of the challenges, which affect virtually all large-scale computational science efforts. To achieve that objective, we assembled a unique team of researchers consisting of representatives from DOE national laboratories, academia, and industry, and engaged in software technology R&D, as well as engaged in close partnerships with DOE science code teams, to produce software technologies that were shown to run effectively at scale on DOE HPC platforms.
In-situ Non-destructive Studies on Biofouling Processes in Reverse Osmosis Membrane Systems
Farhat, Nadia
2016-12-01
Reverse osmosis (RO) and nanofiltration (NF) membrane systems are high-pressure membrane filtration processes that can produce high quality drinking water. Biofouling, biofilm formation that exceeds a certain threshold, is a major problem in spiral wound RO and NF membrane systems resulting in a decline in membrane performance, produced water quality, and quantity. In practice, detection of biofouling is typically done indirectly through measurements of performance decline. Existing direct biofouling detection methods are mainly destructive, such as membrane autopsies, where biofilm samples can be contaminated, damaged and resulting in biofilm structural changes. The objective of this study was to test whether transparent luminescent planar oxygen sensing optodes, in combination with a simple imaging system, can be used for in-situ, non-destructive biofouling characterization. Aspects of the study were early detection of biofouling, biofilm spatial patterning in spacer filled channels, and the effect of feed cross-flow velocity, and feed flow temperature. Oxygen sensing optode imaging was found suitable for studying biofilm processes and gave detailed spatial and quantitative biofilm development information enabling better understanding of the biofouling development process. The outcome of this study attests the importance of in-situ, non-destructive imaging in acquiring detailed knowledge on biofilm development in membrane systems contributing to the development of effective biofouling control strategies.
International Nuclear Information System (INIS)
Wang, Z.; Fingas, M.; Sigouin, L.; Yang, C.; Hollebone, B.
2003-01-01
The assessment, cleanup, and remediation of hydrocarbon contaminated sites is covered in the Reference Method for Canada-Wide Standard for Petroleum Hydrocarbons-Tier 1 Method. It replaces several analytical methods used in the past by some laboratories and jurisdictions in Canada. The authors conducted two comparative evaluations to validate the Tier 1 Analytical Method. The first compared the Internal and External Standard Methods, and the second compared the Silica Gel Column Cleanup Method with the In-situ Silica Gel Cleanup Method. The Canadian Council of Ministers of the Environment (CCME) Tier 1 Method recommends and requires the External Standard Method to determine petroleum hydrocarbons (PHC) in soil samples. The Internal Method is widely used to quantify various organic and inorganic pollutants in environmental samples. The Tier 1 Method offers two options for the same extract cleanup. They are: Option A - In-situ Silica Gel Cleanup, and Option B - Silica Gel Column Cleanup. Linearity, precision, and PHC quantification results were the parameters considered for diesel and motor oil solutions, for diesel spiked soil samples, and for motor oil spiked soil samples. It was concluded that both the External and Internal Standard Methods for gas chromatograph (GC) determination of PHC in soil possess their own advantages. The PHC results obtained using the In-Situ Silica Gel Cleanup Method were lower than those obtained with the Silica Gel Column Cleanup Methods. The more efficient and effective sample cleanup method proved to be the Silica Gel Column Method. 13 refs., 7 tabs., 7 figs
An Improved Spectral Analysis Method for Fatigue Damage Assessment of Details in Liquid Cargo Tanks
Zhao, Peng-yuan; Huang, Xiao-ping
2018-03-01
Errors will be caused in calculating the fatigue damages of details in liquid cargo tanks by using the traditional spectral analysis method which is based on linear system, for the nonlinear relationship between the dynamic stress and the ship acceleration. An improved spectral analysis method for the assessment of the fatigue damage in detail of a liquid cargo tank is proposed in this paper. Based on assumptions that the wave process can be simulated by summing the sinusoidal waves in different frequencies and the stress process can be simulated by summing the stress processes induced by these sinusoidal waves, the stress power spectral density (PSD) is calculated by expanding the stress processes induced by the sinusoidal waves into Fourier series and adding the amplitudes of each harmonic component with the same frequency. This analysis method can take the nonlinear relationship into consideration and the fatigue damage is then calculated based on the PSD of stress. Take an independent tank in an LNG carrier for example, the accuracy of the improved spectral analysis method is proved much better than that of the traditional spectral analysis method by comparing the calculated damage results with the results calculated by the time domain method. The proposed spectral analysis method is more accurate in calculating the fatigue damages in detail of ship liquid cargo tanks.
International Nuclear Information System (INIS)
Behbahani, A.
1983-01-01
An analytical neutron sensor response model and methods for transient response measurements of neutron sensors (compensated ionization chamber), including possible in-situ techniques have been developed and evaluated to meet the provisions of Draft Standard ISA Sd67.06, IEEE 338-1977, and NRC Guide 1.118. One in-situ method requires the perturbation of the high voltage detector (sensor) power supply and measurement of the sensor response. The response to a perturbation of the power supply is related to the response of the sensor to a transient change in neutron flux. Random signal analysis is another in-situ technique to monitor the neutron sensor response. In this method the power spectrum of the inherent fluctuations from the neutron sensor output (current in CIC) are measured and evaluated. Transient response techniques (including in-situ methods) are experimentally and analytically evaluated to identify the mechanisms which may cause degradation in the response of the neutron sensors. The objective of the experimental evaluation was to correlate the measured response time using transient radiation flux changes and power supply perturbation. The objective of the analytical model of the different sensor response was to predict response time and degradation mechanisms
HER2 testing in the UK: recommendations for breast and gastric in-situ hybridisation methods
LENUS (Irish Health Repository)
Bartlett, J. M. S.
2011-01-01
These guidelines supplement existing guidelines on HER2 testing by immunohistochemistry and in-situ hybridisation(ISH) methods in the UK. They provide a specific focus on aspects of guidance relevant to HER2 ISH testing methods, both fluorescent and chromogenic. They are formulated to give advice on methodology, interpretation and quality control for ISH-based testing of HER2 status in common tumour types, including both breast and gastric tumours. The aim is to ensure that all ISH-based testing is accurate, reliable and timely.
Energy Technology Data Exchange (ETDEWEB)
Dixon, Robert A; Lance, P E; Downs, A; Kier, Brian P [EMCON Northwest Inc., Portland, OR (United States)
1994-12-31
Four case studies of successful in-situ remediation are summarized illustrating cost-effective methods to remediate soil and groundwater contaminated with volatile and non-volatile petrochemicals. Each site is in a different geologic environment with varying soil types and with and without groundwater impact. The methods described include vadose zone vapor extraction, high-vacuum vapor extraction combined with groundwater tab.le depression, air sparging with groundwater recovery and vapor extraction, and bio remediation of saturated zone soils using inorganic nutrient and oxygen addition
Energy Technology Data Exchange (ETDEWEB)
Dixon, Robert A.; Lance, P.E.; Downs, A.; Kier, Brian P. [EMCON Northwest Inc., Portland, OR (United States)
1993-12-31
Four case studies of successful in-situ remediation are summarized illustrating cost-effective methods to remediate soil and groundwater contaminated with volatile and non-volatile petrochemicals. Each site is in a different geologic environment with varying soil types and with and without groundwater impact. The methods described include vadose zone vapor extraction, high-vacuum vapor extraction combined with groundwater tab.le depression, air sparging with groundwater recovery and vapor extraction, and bio remediation of saturated zone soils using inorganic nutrient and oxygen addition
The method for the in-situ leaching of a uranium mining
International Nuclear Information System (INIS)
Chen Zhen; Xu Xianyi; Wang Xuemin
2011-01-01
The paper reviews the main factors of in-situ leaching for uranium mining. A kind of technique called dilution with few reagent is put forward to the in-situ leaching of sandstone-type uranium deposit with high TDS. This technique can not only effectively prevent the pipe plug, but also can improve the economic benefits. (authors)
Directory of Open Access Journals (Sweden)
Bingzhen Li
2017-05-01
Full Text Available Fe3O4/PPy/PANI (Fe3O4/polypyrrole/polyaniline nanocomposites with excellent microwave absorbing properties have been successfully synthesized and characterized systematically. In detail, Fe3O4 nanoparticles were prepared via an environmental friendly, modified co-precipitation method. Afterward, two conductive polymers, PPy and PANI, were deposited onto the surface of Fe3O4 nanoparticles by in-situ polymerization of pyrrole and aniline. PPy and PANI was “glued” by the strong affinity between the carbonyl groups of PPy and the conjugated chains of PANI. The obtained Fe3O4/PPy/PANI nanocomposites have been found to possess excellent microwave absorbing property with the absorption bandwidth of 10.7 GHz (6.7–17.4 GHz and maximum reflection loss at 10.1 GHz (−40.2 dB. It proves that the combination of ultra-small Fe3O4 nanoparticles with two different conductive polymers have a great potential in the application of microwave absorbing materials.
Qi, Wei; Yan, Pengqiang; Su, Dang Sheng
2018-03-20
Sustainable and environmentally benign catalytic processes are vital for the future to supply the world population with clean energy and industrial products. The replacement of conventional metal or metal oxide catalysts with earth abundant and renewable nonmetallic materials has attracted considerable research interests in the field of catalysis and material science. The stable and efficient catalytic performance of nanocarbon materials was discovered at the end of last century, and these materials are considered as potential alternatives for conventional metal-based catalysts. With its rapid development in the past 20 years, the research field of carbon catalysis has been experiencing a smooth transition from the discovery of novel nanocarbon materials or related new reaction systems to the atomistic-level mechanistic understanding on the catalytic process and the subsequent rational design of the practical catalytic reaction systems. In this Account, we summarize the recent progress in the kinetic and mechanistic studies on nanocarbon catalyzed alkane oxidative dehydrogenation (ODH) reactions. The paper attempts to extract general concepts and basic regularities for carbon catalytic process directing us on the way for rational design of novel efficient metal-free catalysts. The nature of the active sites for ODH reactions has been revealed through microcalorimetric analysis, ambient pressure X-ray photoelectron spectroscopy (XPS) measurement, and in situ chemical titration strategies. The detailed kinetic analysis and in situ catalyst structure characterization suggests that carbon catalyzed ODH reactions involve the redox cycles of the ketonic carbonyl-hydroxyl pairs, and the key physicochemical parameters (activation energy, reaction order, and rate/equilibrium constants, etc.) of the carbon catalytic systems are proposed and compared with conventional transition metal oxide catalysts. The proposal of the intrinsic catalytic activity (TOF) provides the
DOE In Situ Remediation Integrated Program
International Nuclear Information System (INIS)
Yow, J.L. Jr.
1993-01-01
The In Situ Remediation Integrated Program (ISRP) supports and manages a balanced portfolio of applied research and development activities in support of DOE environmental restoration and waste management needs. ISRP technologies are being developed in four areas: containment, chemical and physical treatment, in situ bioremediation, and in situ manipulation (including electrokinetics). the focus of containment is to provide mechanisms to stop contaminant migration through the subsurface. In situ bioremediation and chemical and physical treatment both aim to destroy or eliminate contaminants in groundwater and soils. In situ manipulation (ISM) provides mechanisms to access contaminants or introduce treatment agents into the soil, and includes other technologies necessary to support the implementation of ISR methods. Descriptions of each major program area are provided to set the technical context of the ISM subprogram. Typical ISM needs for major areas of in situ remediation research and development are identified
Quantitative in situ magnetization reversal studies in Lorentz microscopy and electron holography.
Rodríguez, L A; Magén, C; Snoeck, E; Gatel, C; Marín, L; Serrano-Ramón, L; Prieto, J L; Muñoz, M; Algarabel, P A; Morellon, L; De Teresa, J M; Ibarra, M R
2013-11-01
A generalized procedure for the in situ application of magnetic fields by means of the excitation of the objective lens for magnetic imaging experiments in Lorentz microscopy and electron holography is quantitatively described. A protocol for applying magnetic fields with arbitrary in-plane magnitude and orientation is presented, and a freeware script for Digital Micrograph(™) is provided to assist the operation of the microscope. Moreover, a method to accurately reconstruct hysteresis loops is detailed. We show that the out-of-plane component of the magnetic field cannot be always neglected when performing quantitative measurements of the local magnetization. Several examples are shown to demonstrate the accuracy and functionality of the methods. © 2013 Elsevier B.V. All rights reserved.
Application of tomographics methods for determination of rock failure in situ
Directory of Open Access Journals (Sweden)
Pandula Blažej
1996-03-01
Full Text Available Rock Failure determination closely connects with optimalization of rock disintegration as well excavation of underground openings. The aim of this optimalization is than to reach desired fragmentation during the blasting as well as choose the most suitable excavation technology. Study of rock failure can be very useful to reach this goal. In practice it can be done on the basis of personal technological experiences of blasting operator obtained in long term experiences in situ conditions. Because in many cases that experiences can not be utilised, it is necessary to learn against more precise rock failure evaluation. It can also down the costs of disintegration as well as excavation. The paper deals with results of common geophysical methods and methods of seismic tomography achieved at the hidden rock failure determination in the rock masses of the Ruzin dam during projecting the outlet equipment.
''In situ'' electronic testing method of a neutron detector performance
International Nuclear Information System (INIS)
Gonzalez, J.M.; Levai, F.
1987-01-01
The method allows detection of any important change in the electrical characteristics of a neutron sensor channel. It checks the response signal produced by an electronic detector circuit when a pulse generator is connected as input signal in the high voltage supply. The electronic circuit compares the detector capacitance value, previously measured, against a reference value, which is adjusted in a window type comparator electronic circuit to detect any important degrading condition of the capacitance value in a detector-cable system. The ''in-situ'' electronic testing method of neutron detector performance has been verified in a laboratory atmosphere to be a potential method to detect any significant change in the capacitance value of a nuclear sensor and its connecting cable, also checking: detector disconnections, cable disconnections, length changes of the connecting cable, electric short-opened circuits in the sensor channel, and any electrical trouble in the detector-connector-cable system. The experimental practices were carried out by simulation of several electric changes in a nuclear sensor-cable system from a linear D.C. channel which measures reactor power during nuclear reactor operation. It was made at the Training Reactor Electronic Laboratory. The results and conclusions obtained at the Laboratory were proved, satisfactorily, in the Electronic Instrumentation of Budapest Technical University Training Reactor, Hungary
Scalable Analysis Methods and In Situ Infrastructure for Extreme Scale Knowledge Discovery
Energy Technology Data Exchange (ETDEWEB)
Duque, Earl P.N. [J.M. Smith International, LLC, Rutherford, NJ (United States). DBA Intelligent Light; Whitlock, Brad J. [J.M. Smith International, LLC, Rutherford, NJ (United States). DBA Intelligent Light
2017-08-25
High performance computers have for many years been on a trajectory that gives them extraordinary compute power with the addition of more and more compute cores. At the same time, other system parameters such as the amount of memory per core and bandwidth to storage have remained constant or have barely increased. This creates an imbalance in the computer, giving it the ability to compute a lot of data that it cannot reasonably save out due to time and storage constraints. While technologies have been invented to mitigate this problem (burst buffers, etc.), software has been adapting to employ in situ libraries which perform data analysis and visualization on simulation data while it is still resident in memory. This avoids the need to ever have to pay the costs of writing many terabytes of data files. Instead, in situ enables the creation of more concentrated data products such as statistics, plots, and data extracts, which are all far smaller than the full-sized volume data. With the increasing popularity of in situ, multiple in situ infrastructures have been created, each with its own mechanism for integrating with a simulation. To make it easier to instrument a simulation with multiple in situ infrastructures and include custom analysis algorithms, this project created the SENSEI framework.
Zhou, J.; Danilov, D.; Notten, P.H.L.
2006-01-01
An electrochemical method has been developed for the in situ determination of concentration gradients in the electrolyte of sealed Li-ion batteries by measuring the potential difference between microreference electrodes. Formulas relating the concentration gradient and the potential difference
International Nuclear Information System (INIS)
Munson, D.E.; Matalucci, R.V.
1986-01-01
Large-scale, well-instrumented underground tests to determine in situ thermal/structural response of bedded salt are being constructed in the WIPP facility in southeastern New Mexico. These tests are an essential component of a broad research and development program to resolve thermal/structural issues, to validate long-term prediction methods, and to develop a design basis for a future repository. They are the result of an extensive planning and evaluation procedure to determine the appropriate test configuration. All details of the tests, including background, decisions, design, site operations, and testing organization are explained. These procedures may be useful in developing other in situ tests
Applications of in situ hybridization to plant-improvement
International Nuclear Information System (INIS)
Abbasi, F.M.
2004-01-01
In situ hybridization is a powerful method for characteristic alien addition and substitution lines. RFLP analysis can identify the presence of a particular individual chromosome, but whether they are as a pair or as a single chromosome cannot be determined. In situ hybridization has become established as an essential method in cell and molecular biology. It is able to link DNA sequences with their organization and physical position. The rate of technology-development in the field of in situ hybridization has been rapid: radioactive probes are now rarely used, while labeling methods, fluorochromes, chromosomes and tissue-preparation methods, microscope and imaging technology have all useful in functional genomics and localization of transgenes on the chromosomes. (author)
International Nuclear Information System (INIS)
Dupoux, J.
1981-01-01
The in situ measurement of the efficiency of filtering installations in the French nuclear industry using the soda-fluorescein (uraniun) aerosol method is presented. A brief description and the interest of the method are discussed. Its use for the location of defects in filter elements and for in situ control of casing with tandem HEPA filter stages is described
Solution (in situ leach) mining of uranium: an overview
International Nuclear Information System (INIS)
Kuhaida, A.J. Jr.; Kelly, M.J.
1978-01-01
Increases in the demand for and price of uranium have made in-situ mining an attractive alternative to the open-pit and underground U mining methods. Up to 50% of the known ore-bearing sandstone in the western U.S. can be mined using the in-situ mining method. In-situ mining also offers a significant environmental advantage. Restoration of the contaminated groundwater is discussed
Methods for monitoring corals and crustose coralline algae to quantify in-situ calcification rates
Morrison, Jennifer M.; Kuffner, Ilsa B.; Hickey, T. Don
2013-01-01
The potential effect of global climate change on calcifying marine organisms, such as scleractinian (reef-building) corals, is becoming increasingly evident. Understanding the process of coral calcification and establishing baseline calcification rates are necessary to detect future changes in growth resulting from climate change or other stressors. Here we describe the methods used to establish a network of calcification-monitoring stations along the outer Florida Keys Reef Tract in 2009. In addition to detailing the initial setup and periodic monitoring of calcification stations, we discuss the utility and success of our design and offer suggestions for future deployments. Stations were designed such that whole coral colonies were securely attached to fixed apparati (n = 10 at each site) on the seafloor but also could be easily removed and reattached as needed for periodic weighing. Corals were weighed every 6 months, using the buoyant weight technique, to determine calcification rates in situ. Sites were visited in May and November to obtain winter and summer rates, respectively, and identify seasonal patterns in calcification. Calcification rates of the crustose coralline algal community also were measured by affixing commercially available plastic tiles, deployed vertically, at each station. Colonization by invertebrates and fleshy algae on the tiles was low, indicating relative specificity for the crustose coralline algal community. We also describe a new, nonlethal technique for sampling the corals, used following the completion of the monitoring period, in which two slabs were obtained from the center of each colony. Sampled corals were reattached to the seafloor, and most corals had completely recovered within 6 months. The station design and sampling methods described herein provide an effective approach to assessing coral and crustose coralline algal calcification rates across time and space, offering the ability to quantify the potential effects of
Mirza, Tahseen; Liu, Qian Julie; Vivilecchia, Richard; Joshi, Yatindra
2009-03-01
There has been a growing interest during the past decade in the use of fiber optics dissolution testing. Use of this novel technology is mainly confined to research and development laboratories. It has not yet emerged as a tool for end product release testing despite its ability to generate in situ results and efficiency improvement. One potential reason may be the lack of clear validation guidelines that can be applied for the assessment of suitability of fiber optics. This article describes a comprehensive validation scheme and development of a reliable, robust, reproducible and cost-effective dissolution test using fiber optics technology. The test was successfully applied for characterizing the dissolution behavior of a 40-mg immediate-release tablet dosage form that is under development at Novartis Pharmaceuticals, East Hanover, New Jersey. The method was validated for the following parameters: linearity, precision, accuracy, specificity, and robustness. In particular, robustness was evaluated in terms of probe sampling depth and probe orientation. The in situ fiber optic method was found to be comparable to the existing manual sampling dissolution method. Finally, the fiber optic dissolution test was successfully performed by different operators on different days, to further enhance the validity of the method. The results demonstrate that the fiber optics technology can be successfully validated for end product dissolution/release testing. (c) 2008 Wiley-Liss, Inc. and the American Pharmacists Association
Fresco, Jacques R.; Johnson, Marion D.
2002-01-01
Disclosed are methods for detecting in situ the presence of a target sequence in a substantially double-stranded nucleic acid segment, which comprises: a) contacting in situ under conditions suitable for hybridization a substantially double-stranded nucleic acid segment with a detectable third strand, said third strand being capable of hybridizing to at least a portion of the target sequence to form a triple-stranded structure, if said target sequence is present; and b) detecting whether hybridization between the third strand and the target sequence has occured.
International Nuclear Information System (INIS)
Symons, G.
1985-12-01
A detailed investigation of background radiation levels near the reef zone in the uranium section of the Western Areas Mine was conducted using a collimated radiometric face scanner. This study demonstrated that these radiation levels can be high; 25% or more of the counts measured when sampling a reef face may originate from a background source, especially from uranium ore rubble on the footwall close to the reef face. A method using a 20mm frontal shield was devised to obtain an accurate background correction. Three calibration schemes, the Area method, the Gamlog method, and the Deconvolution method were implemented for the production of accurate in-situ radiometric uranium grades. This involved the construction of a step-response calibration pad at Pelindaba together with the establisment of appropriate software and underground radiometric sampling procedures. Radiometric grades generated by these calibration procedures from 60 channel sections were on average 10% below those procured from conventional chip sampling. A correlation between gold and uranium grades was also evident. Crushed rock samples were collected to investigate the thorium problem and are still undergoing analysis at the time of writing. Refinements in the design of the collimated face scanner are also described
International Nuclear Information System (INIS)
1994-02-01
This special study was conducted to prepare a guidance document for selecting in situ hydraulic conductivity (K) tests, comparing in situ testing methods, and evaluating the results of such tests. This report may be used as a practical decision-making tool by the Uranium Mill Tailings Remedial Action (UMTRA) Project staff to determine which testing method will most efficiently achieve the field-saturated K results needed for long-term planning. A detailed section on near-surface test methods discusses each method which may be applicable to characterization of UMTRA disposal cell covers, liners and foundation materials. These potentially applicable test methods include the sealed double-ring infiltrometer (SDRI), the air-entry permeameter (AEP), the guelph permeameter, the two-stage borehole technique (TSB), the pressure infiltrometer, and the disk permeameter. Analytical solutions for these methods are provided, and limitations of these solutions are discussed, and a description of testing equipment design and installation are provided
Ductal carcinoma in situ: a proposal for a new classification
Holland, R.; Peterse, J. L.; Millis, R. R.; Eusebi, V.; Faverly, D.; van de Vijver, M. J.; Zafrani, B.
1994-01-01
Details of a proposed new classification for ductal carcinoma in situ (DCIS) are presented. This is based, primarily, on cytonuclear differentiation and, secondarily, on architectural differentiation (cellular polarisation). Three categories are defined. First is poorly differentiated DCIS composed
International Nuclear Information System (INIS)
Toguri, Satohito; Asano, Hidekazu; Takao, Hajime; Matsuda, Takeshi; Amemiya, Kiyoshi
2008-01-01
(i) Bentonite Block: Applicability of manufacturing technology of buffer material was verified by manufacturing of full scale bentonite ring which consists of one-eight (1/8) dividing block (Outside Diameter (OD): 2.220 mm H: 300 mm). Density characteristic, dimension and scale effect, which were considered the tunnel environment under transportation, were evaluated. Vacuum suction technology was selected as handling technology for the ring. Hoisting characteristic of vacuum suction technology was presented through evaluation of the mechanical property of buffer material, the friction between blocks, etc. by using a full-scale bentonite ring (OD 2.200 mm, H 300 mm). And design of bentonite block and emplacement equipment were presented in consideration of manufacturability of the block, stability of handling and improvement of emplacement efficiency. (ii) Bentonite Pellet Filling: Basic characteristics such as water penetration, swelling and thermal conductivity of various kinds of bentonite pellet were collected by laboratory scale tests. Applicability of pellet filling technology was evaluated by horizontal filling test using a simulated full-scale drift tunnel (OD 2.200 mm, L 6 m) . Filling density, grain size distribution, etc. were also measured. (iii) In-Situ Compaction of Bentonite: Dynamic compaction method (heavy weight fall method) was selected as in-situ compaction technology. Compacting examination which used a full scale disposal pit (OD 2.360 mm) was carried out. Basic specification of compacting equipment and applicability of in-situ compaction technology were presented. Density, density distribution of buffer material and energy acted on the wall of the pit, were also measured. (author)
International Nuclear Information System (INIS)
Taneja, K.; Singer, R.
1987-01-01
In situ hybridization is a useful method for localizing specific nucleic acid sequences intracellularly and for studying regulation of gene expression. Recently synthetic oligonucleotides have been successfully used as probes in this technique. Since they can be made easily to specific nucleic acid regions, they may be the best approach for analysis of a gene family of highly conserved sequences. They have analyzed these probes for the development of an in situ hybridization method. Oligonucleotides were made to different regions of chick beta-actin mRNA and used for detection of these sequences in a culture of chicken fibroblasts and myoblasts. They found that synthetic DNAs have different efficiencies of hybridization, indicating that not all target sequences are equivalent. They have investigated in detail a particular probe to the actin mRNA coding region and have optimized hybridization parameters. When hybridization was quantitated it was found that an oligonucleotide end labelled with 35 S or 32 P was capable of detecting several thousand messages per cell with a signal-to-noise ratio of 10:1. In situ hybridization confirmed the specificity of the hybridization as well as the background level. Increase in the number of oligonucleotides used should increase the signal-to-noise ratio-proportionately. Under particular circumstances the specificity of oligonucleotides make them an important reagent for in situ hybridization
Pattern classification by memristive crossbar circuits using ex situ and in situ training
Alibart, Fabien; Zamanidoost, Elham; Strukov, Dmitri B.
2013-06-01
Memristors are memory resistors that promise the efficient implementation of synaptic weights in artificial neural networks. Whereas demonstrations of the synaptic operation of memristors already exist, the implementation of even simple networks is more challenging and has yet to be reported. Here we demonstrate pattern classification using a single-layer perceptron network implemented with a memrisitive crossbar circuit and trained using the perceptron learning rule by ex situ and in situ methods. In the first case, synaptic weights, which are realized as conductances of titanium dioxide memristors, are calculated on a precursor software-based network and then imported sequentially into the crossbar circuit. In the second case, training is implemented in situ, so the weights are adjusted in parallel. Both methods work satisfactorily despite significant variations in the switching behaviour of the memristors. These results give hope for the anticipated efficient implementation of artificial neuromorphic networks and pave the way for dense, high-performance information processing systems.
Energy Technology Data Exchange (ETDEWEB)
Dutta, Abhijit [National Renewable Energy Lab. (NREL), Golden, CO (United States); Sahir, A. H. [National Renewable Energy Lab. (NREL), Golden, CO (United States); Tan, Eric [National Renewable Energy Lab. (NREL), Golden, CO (United States); Humbird, David [DWH Process Consulting, Denver, CO (United States); Snowden-Swan, Lesley J. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Meyer, Pimphan A. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Ross, Jeff [Harris Group, Inc., Seattle, WA (United States); Sexton, Danielle [Harris Group, Inc., Seattle, WA (United States); Yap, Raymond [Harris Group, Inc., Seattle, WA (United States); Lukas, John [Harris Group, Inc., Seattle, WA (United States)
2015-03-01
This report was developed as part of the U.S. Department of Energy’s Bioenergy Technologies Office’s efforts to enable the development of technologies for the production of infrastructure-compatible, cost-competitive liquid hydrocarbon fuels from biomass. Specifically, this report details two conceptual designs based on projected product yields and quality improvements via catalyst development and process integration. It is expected that these research improvements will be made within the 2022 timeframe. The two conversion pathways detailed are (1) in situ and (2) ex situ upgrading of vapors produced from the fast pyrolysis of biomass. While the base case conceptual designs and underlying assumptions outline performance metrics for feasibility, it should be noted that these are only two of many other possibilities in this area of research. Other promising process design options emerging from the research will be considered for future techno-economic analysis. Both the in situ and ex situ conceptual designs, using the underlying assumptions, project MFSPs of approximately $3.5/gallon gasoline equivalent (GGE). The performance assumptions for the ex situ process were more aggressive with higher distillate (diesel-range) products. This was based on an assumption that more favorable reaction chemistry (such as coupling) can be made possible in a separate reactor where, unlike in an in situ upgrading reactor, one does not have to deal with catalyst mixing with biomass char and ash, which pose challenges to catalyst performance and maintenance. Natural gas was used for hydrogen production, but only when off gases from the process was not sufficient to meet the needs; natural gas consumption is insignificant in both the in situ and ex situ base cases. Heat produced from the burning of char, coke, and off-gases allows for the production of surplus electricity which is sold to the grid allowing a reduction of approximately 5¢/GGE in the MFSP.
Assessment of microbial in situ activity in contaminated aquifers
Energy Technology Data Exchange (ETDEWEB)
Kaestner, M. [UFZ-Umweltforschungszentrum Leipzig-Halle GmbH, Department Bioremediation, Permoserstrasse 15, 04318 Leipzig (Germany); Fischer, A.; Nijenhuis, I.; Stelzer, N.; Bombach, P.; Richnow, H.H. [UFZ-Umweltforschungszentrum Leipzig-Halle GmbH, Department Isotopenbiogeochemie, Permoserstrasse 15, 04318 Leipzig (Germany); Geyer, R. [UFZ-Umweltforschungszentrum Leipzig-Halle GmbH, Department Umweltmikrobiologie, Permoserstrasse 15, 04318 Leipzig (Germany); Tebbe, C.C. [Institut fuer Agraroekologie, Bundesforschungsanstalt fuer Landwirtschaft (FAL), D-38116 Braunschweig (Germany)
2006-06-15
Microbial ecologists and environmental engineers share the interest in identifying the key microorganisms responsible for compound turnover in the environment and in estimating the respective transformation rates. For the successful application of Natural Attenuation processes, a reliable assessment of the in situ turnover of a contaminant in an aquifer is essential. Here, we review and present new details of two recently developed approaches concerning the assessment of in situ biodegradation: (i) determination of biodegradation caused by microbial metabolism in a contamination plume by stable isotope fractionation analysis (SIFA) and (ii) determination of the actual degradation under the respective environmental conditions in the aquifer by using in situ microcosms (BACTRAPS registered) amended with {sup 13}C-labeled substrates as tracer compounds. Based on stable isotope fractionation analysis, the degradation occurring under anoxic biogeochemical conditions at a respective site can be calculated for the entire plume. This has been shown for benzene and toluene at the Zeitz site and partly for chlorobenzene at the Bitterfeld site. By use of the in situ microcosm approach with {sup 13}C-labeled compounds, the microbial in situ degradation under strictly anaerobic conditions could be proven for benzene and toluene in Zeitz and for chlorobenzene in Bitterfeld. The transformation of {sup 13}C-carbon of the labeled substrate into microbial fatty acids confirmed the assimilation of the pollutant resulting in the formation of biomass. In addition, metabolites such as benzylsuccinic acid were found in the toluene-amended microcosms indicating anaerobic degradation of toluene. This result corresponds to the geochemical conditions found at the field site and therefore, the microcosm approach with {sup 13}C-labeled compounds can be used to assign the predominant in situ degradation pathways in a contaminated aquifer. Since fatty acids profiles alone are often too
In Situ Gelation-Induced Death of Cancer Cells Based on Proteinosomes.
Zhou, Yuting; Song, Jianmin; Wang, Lei; Xue, Xuting; Liu, Xiaoman; Xie, Hui; Huang, Xin
2017-08-14
Hydrogels are an excellent type of material that can be utilized as a platform for cell culture. However, when a bulky hydrogel forms on the inside of cancer cells, the result would be different. In this study, we demonstrate a method for in situ gelation inside cancer cells that can efficiently induce cell death. Glutathione-responsive proteinosomes with good biocompatibility were prepared as carriers for sodium alginate to be endocytosed by cancer cells, where the chelation between sodium alginate and free calcium ions in the culture medium occurs during the diffusion process. The uptake of the hydrogel-loaded proteinosomes into the cancer cells, and then the triggered release of hydrogel with concomitant aggregation, was well-confirmed by monitoring the change of the Young's modulus of the cells based on AFM force measurements. Accordingly, when a large amount of hydrogel formed in cells, the cell viability would be inhibited by ∼90% by MTT assay at a concentration of 5.0 μM of hydrogel-loaded proteinosomes after 48 h incubation, which clearly proves the feasibility of the demonstrated method for killing cancer cells. Although more details regarding the mechanism of cell death should be conducted in the near future, such a demonstrated method of in situ gelation inside cells provides another choice for killing cancer cells.
Energy Technology Data Exchange (ETDEWEB)
Boehm, Benny [Technical Univ. of Denmark, Dept. of Energy Engineering (Denmark)
1996-11-01
A comparison of different methods for in-situ determination of heat losses has been carried out on a 273 mm transmission line in Copenhagen. Instrumentation includes temperature sensors, heat flux meters and an infrared camera. The methods differ with regard to time consumption and costs of applying the specific method, demand on accuracy of temperature measurements, sensitivity to computational parameters, e.g. the thermal conductivity of the soil, response to transients in water temperature and the ground, and steady state assumptions in the model used in the interpretation of the measurements. Several of the applied methods work well. (au)
Energy Technology Data Exchange (ETDEWEB)
O' Leary, Patrick [Kitware, Inc., Clifton Park, NY (United States)
2017-09-13
The primary challenge motivating this project is the widening gap between the ability to compute information and to store it for subsequent analysis. This gap adversely impacts science code teams, who can perform analysis only on a small fraction of the data they calculate, resulting in the substantial likelihood of lost or missed science, when results are computed but not analyzed. Our approach is to perform as much analysis or visualization processing on data while it is still resident in memory, which is known as in situ processing. The idea in situ processing was not new at the time of the start of this effort in 2014, but efforts in that space were largely ad hoc, and there was no concerted effort within the research community that aimed to foster production-quality software tools suitable for use by Department of Energy (DOE) science projects. Our objective was to produce and enable the use of production-quality in situ methods and infrastructure, at scale, on DOE high-performance computing (HPC) facilities, though we expected to have an impact beyond DOE due to the widespread nature of the challenges, which affect virtually all large-scale computational science efforts. To achieve this objective, we engaged in software technology research and development (R&D), in close partnerships with DOE science code teams, to produce software technologies that were shown to run efficiently at scale on DOE HPC platforms.
Visualization of Enzyme Activities in Earthworm Biopores by In Situ Soil Zymography.
Razavi, Bahar S; Hoang, Duyen; Kuzyakov, Yakov
2017-01-01
Earthworms produce biopores with strongly increased microbial and enzyme activities and consequently they form microbial hotspots in soil. In extremely dynamic microhabitats and hotspots such as earthworm biopores, the in situ enzyme activities are a footprint of process rates and complex biotic interactions. The effect of earthworms on enzyme activities inside biopores, relative to earthworm-free soil, can be visualized by in situ soil zymography. Here, we describe the details of the approach and discuss its advantages and limitations. Direct zymography provides high spatial resolution for quantitative images of enzyme activities in biopores.
Energy Technology Data Exchange (ETDEWEB)
Meister, H., E-mail: meister@ipp.mpg.de; Penzel, F.; Giannone, L.; Kannamueller, M.; Kling, A.; Koll, J.; Trautmann, T.
2011-10-15
In order to derive the local emission profile of the plasma radiation in a fusion device using the line-integrated measurements of the bolometer diagnostic, tomographic reconstruction methods have to be applied to the measurements from many lines-of-sight. A successful reconstruction needs to take the finite sizes of detectors and apertures and the resulting non-ideal measurements into account. In ITER a method for in situ measurement of the geometrical properties of the various components of the bolometer diagnostic after installation is required as the viewing cones have to pass through narrow gaps between components. The method proposed to be used for ITER uses the beam of a laser with high intensity to illuminate the bolometer assembly from many different angles {xi} and {theta}. A light-weight robot from Kuka Robotics is used to efficiently position the laser on many points covering the complete viewing cone of each line-of-sight and to direct the beam precisely into the entrance aperture of the bolometer. Measuring the response of the bolometer allows for the calculation of the transmission function t({xi}, {theta}), the angular etendue and finally the geometric function in reconstruction space, which is required for the tomography algorithms. Measuring the transmission function for a laboratory assembly demonstrates the viability of the proposed method. Results for a collimator-type camera from a prototype envisaged for ITER are presented. The implemented procedure is discussed in detail, in particular with respect to the automatisation applied which takes the achievable positioning and alignment accuracies of the robot into account. This discussion is extended towards the definition of requirements for a remote-handling tool for ITER.
Directory of Open Access Journals (Sweden)
M. R. Bilad
2016-05-01
Full Text Available A novel in-situ enzymatic cleaning method was developed for fouling control in membrane bioreactors (MBRs. It is achieved by bringing the required enzymes near the membrane surface by pulling the enzymes to a magnetic membrane (MM surface by means of magnetic forces, exactly where the cleaning is required. To achieve this, the enzyme was coupled to a magnetic nanoparticle (MNP and the membrane it self was loaded with MNP. The magnetic activity was turned by means of an external permanent magnet. The effectiveness of concept was tested in a submerged membrane filtration using the model enzyme-substrate of Bacillus subitilis xylanase-arabinoxylan. The MM had almost similar properties compared to the unloaded ones, except for its well distributed MNPs. The enzyme was stable during coupling conditions and the presence of coupling could be detected using a high-performance anion-exchange chromatography (HPAEC analysis and Fourier transform infrared spectroscopy (FTIR. The system facilitated an in-situ enzymatic cleaning and could be effectively applied for control fouling in membrane bioreactors (MBRs.
DEFF Research Database (Denmark)
van Rijk, A.; Svenstroup-Poulsen, T.; Jones, M.
2010-01-01
within the reach of every pathology laboratory. Design and Methods Our study was initiated to determine the consistency between chromogenic in situ hybridization and fluorescence in situ hybridization, both using split-signal probes developed for the detection of chromosomal breaks. Five hundred...... and actual signal were compared to the original fluorescence hybridization results. In addition, hematoxylin background staining intensity and signal intensity of the double-staining chromogenic in situ hybridization procedure were analyzed. Results With respect to the presence or absence of chromosomal...
Nanoparticles laden in situ gelling system for ocular drug targeting
Directory of Open Access Journals (Sweden)
Divya Kumar
2013-01-01
Full Text Available Designing an ophthalmic drug delivery system is one of the most difficult challenges for the researchers. The anatomy and physiology of eye create barriers like blinking which leads to the poor retention time and penetration of drug moiety. Some conventional ocular drug delivery systems show shortcomings such as enhanced pre-corneal elimination, high variability in efficiency, and blurred vision. To overcome these problems, several novel drug delivery systems such as liposomes, nanoparticles, hydrogels, and in situ gels have been developed. In situ-forming hydrogels are liquid upon instillation and undergo phase transition in the ocular cul-de-sac to form viscoelastic gel and this provides a response to environmental changes. In the past few years, an impressive number of novel temperature, pH, and ion-induced in situ-forming systems have been reported for sustain ophthalmic drug delivery. Each system has its own advantages and drawbacks. Thus, a combination of two drug delivery systems, i.e., nanoparticles and in situ gel, has been developed which is known as nanoparticle laden in situ gel. This review describes every aspects of this novel formulation, which present the readers an exhaustive detail and might contribute to research and development.
Bhargava, Rohit; Oppenheimer, Orit; Gerald, William; Jhanwar, Suresh C; Chen, Beiyun
2005-06-01
Chromogenic in situ hybridization (CISH) is a recently developed technique, which utilizes the general principles of in situ hybridization and a detection system similar to immunohistochemistry. To assess the utility of CISH for analysis of MYCN gene amplification, we compared this assay with established diagnostic assays such as Southern blot analysis (SB) and fluorescent in situ hybridization (FISH). CISH was performed on 67 cases of neuroblastoma using tissue microarray (65 cases) and whole tissue sections (2 cases). Unequivocal, high-level amplification (> or =10 gene copies per tumor nucleus) was identified in 19 of 67 (28.4%) tumors. Two (3%) tumors showed low-level amplification (6-9 gene copies per tumor nucleus). No amplification was seen in 46 of 67 (68.6%) tumors. SB data were available in 44 tumors. Forty-one of the 44 tumors (93%) showed concordant results between CISH and SB. Three tumors showed MYCN amplification by CISH but no amplification by SB, most likely due to dilution effect of nonneoplastic tissue in the test samples. Two of these three tumors also showed MYCN amplification by FISH, and the third tumor was not analyzed by FISH. FISH data were available in total of 30 tumors. All 30 tumors showed concordant results between CISH and FISH for classifying a tumor as MYCN amplified or not amplified. We conclude that CISH is an accurate method for determining MYCN gene amplification, with added advantages that make it a more practically useful method.
Energy Technology Data Exchange (ETDEWEB)
Raeaef, C.L. [Malmoe Univ. Hospital, Lund Univ., Dept. of Radiation Physics (Sweden)
1997-12-31
In the beginning of 1996 the Department of Radiation Physics in Malmoe was commissioned by the Swedish Radiation Protection Institute to perform a compilation of the results obtained by the Swedish in-situ teams that participated in the RESUME-95 exercise. The aim of this survey is to study the coherence in the reported activity data between the groups. It is not the purpose to see this comparison as a performance ranking of individual laboratories. Any such comparison must be made with precaution since the teams generally have collected their data with different equipment and by different methods. In this work, all in-situ teams have been given code-names, where each team has been labelled a number from 1 to 5. For more details, the interested reader is referred to the internal reports made by each team that (supposedly) also are to be included in the major compilation of the in-situ gamma spectrometry in the RESUME-95 exercise by Danish Emergency Management Agency. (au).
International Nuclear Information System (INIS)
Raeaef, C.L.
1997-01-01
In the beginning of 1996 the Department of Radiation Physics in Malmoe was commissioned by the Swedish Radiation Protection Institute to perform a compilation of the results obtained by the Swedish in-situ teams that participated in the RESUME-95 exercise. The aim of this survey is to study the coherence in the reported activity data between the groups. It is not the purpose to see this comparison as a performance ranking of individual laboratories. Any such comparison must be made with precaution since the teams generally have collected their data with different equipment and by different methods. In this work, all in-situ teams have been given code-names, where each team has been labelled a number from 1 to 5. For more details, the interested reader is referred to the internal reports made by each team that (supposedly) also are to be included in the major compilation of the in-situ gamma spectrometry in the RESUME-95 exercise by Danish Emergency Management Agency. (au)
Energy Technology Data Exchange (ETDEWEB)
Raeaef, C L [Malmoe Univ. Hospital, Lund Univ., Dept. of Radiation Physics (Sweden)
1998-12-31
In the beginning of 1996 the Department of Radiation Physics in Malmoe was commissioned by the Swedish Radiation Protection Institute to perform a compilation of the results obtained by the Swedish in-situ teams that participated in the RESUME-95 exercise. The aim of this survey is to study the coherence in the reported activity data between the groups. It is not the purpose to see this comparison as a performance ranking of individual laboratories. Any such comparison must be made with precaution since the teams generally have collected their data with different equipment and by different methods. In this work, all in-situ teams have been given code-names, where each team has been labelled a number from 1 to 5. For more details, the interested reader is referred to the internal reports made by each team that (supposedly) also are to be included in the major compilation of the in-situ gamma spectrometry in the RESUME-95 exercise by Danish Emergency Management Agency. (au).
International Nuclear Information System (INIS)
Huang Zanjun; Yang Bin; Cui Hua; Zhang Jishan
2003-01-01
A new idea to fabricate aluminum matrix composites strengthened by combined in-situ particle strengthening and in-situ alloying has been proposed. Following the concept of in-situ alloying and in-situ particle strengthening, aluminum matrix composites reinforced by Cu and α-Al 2 O 3 particulate (material I) and the same matrix reinforced by Cu, Si alloying elements and α-Al 2 O 3 particulate (material II) have been obtained. SEM observation, EDS and XRD analysis show that the alloy elements Cu and Si exist in the two materials, respectively. In-situ Al 2 O 3 particulates are generally spherical and their mean size is less than 0.5 μm. TEM observation shows that the in-situ α-Al 2 O 3 particulates have a good cohesion with the matrix. The reaction mechanism of the Al 2 O 3 particulate obtained by this method was studied. Thermodynamic considerations are given to the in-situ reactions and the distribution characteristic of in-situ the α-Al 2 O 3 particulate in the process of solidification is also discussed
International Nuclear Information System (INIS)
Braeckevelt, Mareike; Rokadia, Hemal; Imfeld, Gwenael; Stelzer, Nicole; Paschke, Heidrun; Kuschk, Peter; Kaestner, Matthias; Richnow, Hans-H.; Weber, Stefanie
2007-01-01
The degradation of monochlorobenzene (MCB) was assessed in a constructed wetland treating MCB contaminated groundwater using a detailed geochemical characterisation, stable isotope composition analysis and in situ microcosm experiments. A correlation between ferrous iron mobilisation, decreasing MCB concentration and enrichment in carbon isotope composition was visible at increasing distance from the inflow point, indicating biodegradation of MCB in the wetland. Additionally, in situ microcosm systems loaded with 13 C-labelled MCB were deployed for the first time in sediments to investigate the biotransformation of MCB. Incorporation of 13 C-labelled carbon derived from the MCB into bacterial fatty acids substantiated in situ degradation of MCB. The detection of 13 C-labelled benzene indicated reductive dehalogenation of MCB. This integrated approach indicated the natural attenuation of the MCB in a wetland system. Further investigations are required to document and optimise the in situ biodegradation of MCB in constructed and natural wetland systems treating contaminated groundwater. - An integrated approach including isotope composition analysis and in situ microcosm experiments provided evidences for in situ biodegradation of MCB in a wetland system
Energy Technology Data Exchange (ETDEWEB)
Braeckevelt, Mareike [Departments of Bioremediation, Helmholtz Centre for Environmental Research - UFZ, Permoserstrasse 15, Leipzig D-04318, Saxonia (Germany); Rokadia, Hemal [Department of Isotope Biogeochemistry, Helmholtz Centre for Environmental Research - UFZ, Permoserstrasse 15, Leipzig D-04318, Saxonia (Germany); Imfeld, Gwenael [Department of Isotope Biogeochemistry, Helmholtz Centre for Environmental Research - UFZ, Permoserstrasse 15, Leipzig D-04318, Saxonia (Germany)]. E-mail: gwenael.imfeld@ufz.de; Stelzer, Nicole [Department of Isotope Biogeochemistry, Helmholtz Centre for Environmental Research - UFZ, Permoserstrasse 15, Leipzig D-04318, Saxonia (Germany); Paschke, Heidrun [Department of Analytical Chemistry, Helmholtz Centre for Environmental Research - UFZ, Permoserstrasse 15, Leipzig D-04318, Saxonia (Germany); Kuschk, Peter [Departments of Bioremediation, Helmholtz Centre for Environmental Research - UFZ, Permoserstrasse 15, Leipzig D-04318, Saxonia (Germany); Kaestner, Matthias [Departments of Bioremediation, Helmholtz Centre for Environmental Research - UFZ, Permoserstrasse 15, Leipzig D-04318, Saxonia (Germany); Richnow, Hans-H. [Department of Isotope Biogeochemistry, Helmholtz Centre for Environmental Research - UFZ, Permoserstrasse 15, Leipzig D-04318, Saxonia (Germany); Weber, Stefanie [Department of Isotope Biogeochemistry, Helmholtz Centre for Environmental Research - UFZ, Permoserstrasse 15, Leipzig D-04318, Saxonia (Germany)
2007-07-15
The degradation of monochlorobenzene (MCB) was assessed in a constructed wetland treating MCB contaminated groundwater using a detailed geochemical characterisation, stable isotope composition analysis and in situ microcosm experiments. A correlation between ferrous iron mobilisation, decreasing MCB concentration and enrichment in carbon isotope composition was visible at increasing distance from the inflow point, indicating biodegradation of MCB in the wetland. Additionally, in situ microcosm systems loaded with {sup 13}C-labelled MCB were deployed for the first time in sediments to investigate the biotransformation of MCB. Incorporation of {sup 13}C-labelled carbon derived from the MCB into bacterial fatty acids substantiated in situ degradation of MCB. The detection of {sup 13}C-labelled benzene indicated reductive dehalogenation of MCB. This integrated approach indicated the natural attenuation of the MCB in a wetland system. Further investigations are required to document and optimise the in situ biodegradation of MCB in constructed and natural wetland systems treating contaminated groundwater. - An integrated approach including isotope composition analysis and in situ microcosm experiments provided evidences for in situ biodegradation of MCB in a wetland system.
DEFF Research Database (Denmark)
Hancock, Michael Halloway; Bay, Niels
2007-01-01
, 150 and 210 mm in each step. The investigation has shown that the in situ powder is more readily compacted than the ex situ powder, with an average increase of relative density after mechanical processing of 37% for in situ powder and 19% for ex situ powder. Statistical analysis showed that the choice......Applying the powder in tube (PIT) method, single-filament MgB2/Fe wire and tape has been manufactured applying both the ex situ and the in situ approach. The influence of the roll diameter in three-step flat rolling on the powder density and critical temperature has been examined using rolls of 70...... roll in the first and second reductions followed by the 150 mm or 210 mm roll in the last reduction was the optimum strategy for both powder types. AC susceptibility testing showed that for the in situ tapes there was no correlation between the powder density and the critical temperature. For ex situ...
Detailed modeling of mountain wave PSCs
Directory of Open Access Journals (Sweden)
S. Fueglistaler
2003-01-01
Full Text Available Polar stratospheric clouds (PSCs play a key role in polar ozone depletion. In the Arctic, PSCs can occur on the mesoscale due to orographically induced gravity waves. Here we present a detailed study of a mountain wave PSC event on 25-27 January 2000 over Scandinavia. The mountain wave PSCs were intensively observed by in-situ and remote-sensing techniques during the second phase of the SOLVE/THESEO-2000 Arctic campaign. We use these excellent data of PSC observations on 3 successive days to analyze the PSCs and to perform a detailed comparison with modeled clouds. We simulated the 3-dimensional PSC structure on all 3 days with a mesoscale numerical weather prediction (NWP model and a microphysical box model (using best available nucleation rates for ice and nitric acid trihydrate particles. We show that the combined mesoscale/microphysical model is capable of reproducing the PSC measurements within the uncertainty of data interpretation with respect to spatial dimensions, temporal development and microphysical properties, without manipulating temperatures or using other tuning parameters. In contrast, microphysical modeling based upon coarser scale global NWP data, e.g. current ECMWF analysis data, cannot reproduce observations, in particular the occurrence of ice and nitric acid trihydrate clouds. Combined mesoscale/microphysical modeling may be used for detailed a posteriori PSC analysis and for future Arctic campaign flight and mission planning. The fact that remote sensing alone cannot further constrain model results due to uncertainities in the interpretation of measurements, underlines the need for synchronous in-situ PSC observations in campaigns.
Philipp, W. H.; Hsu, L. C.
1979-01-01
Three methods of in situ cross-linking polyvinyl alcohol films are presented. They are: (1) acetalization with a dialdehyde such as glutaraldehyde, (2) acetalization with aldehyde groups formed by selective oxidative cleaving of the few percent of 1,2 diol units present in polyvinyl alcohol, and (3) cross-linking by hydrogen abstraction by reaction with hydrogen atoms and hydroxyl radicals from irradiated water. For the third method, improvement in film conductivity in KOH solution at the expense of mechanical strength is obtained by the presence of polyacrylic acid in the polyvinyl alcohol films. Resistivities in 45 percent KOH are given for in situ cross-linked films prepared by each of the three methods.
In Situ TEM Electrical Measurements
DEFF Research Database (Denmark)
Canepa, Silvia; Alam, Sardar Bilal; Ngo, Duc-The
2016-01-01
understanding of complex physical and chemical interactions in the pursuit to optimize nanostructure function and device performance. Recent developments of sample holder technology for TEM have enabled a new field of research in the study of functional nanomaterials and devices via electrical stimulation...... influence the sample by external stimuli, e.g. through electrical connections, the TEM becomes a powerful laboratory for performing quantitative real time in situ experiments. Such TEM setups enable the characterization of nanostructures and nanodevices under working conditions, thereby providing a deeper...... and measurement of the specimen. Recognizing the benefits of electrical measurements for in situ TEM, many research groups have focused their effort in this field and some of these methods have transferred to ETEM. This chapter will describe recent advances in the in situ TEM investigation of nanostructured...
Thermodynamics parameters of nano-Ni/PS composites prepared by in situ polymerization method
International Nuclear Information System (INIS)
Liao Qilong; Xiong Jie; Ning Haixia
2011-01-01
Spherical nickel nanoparticles with about 75∼200 nm in size were obtained by a liquid reduction method. The nickel nanoparticles/PS composites were synthesized via in situ polymerization method. XRD, FTIR, SEM and TG-DSC were respectively used to measure the properties of nickel nanoparticles, the microstructure of as-prepared composites samples, the distribution of nickel nanoparticles in PS and the thermodynamic parameters of as-prepared composites. The results show that the nickel nanoparticles will enhance the glass transition temperature of nano-Ni/PS composites. The enthalpy of composites is heightened by increasing of doping dose, and it reaches the top when the doping dose is from 1% to 2%. The specific heat of the composites will reduce with the doping dose of nickel nanoparticles increasing. (authors)
In situ surface roughness measurement using a laser scattering method
Tay, C. J.; Wang, S. H.; Quan, C.; Shang, H. M.
2003-03-01
In this paper, the design and development of an optical probe for in situ measurement of surface roughness are discussed. Based on this light scattering principle, the probe which consists of a laser diode, measuring lens and a linear photodiode array, is designed to capture the scattered light from a test surface with a relatively large scattering angle ϕ (=28°). This capability increases the measuring range and enhances repeatability of the results. The coaxial arrangement that incorporates a dual-laser beam and a constant compressed air stream renders the proposed system insensitive to movement or vibration of the test surface as well as surface conditions. Tests were conducted on workpieces which were mounted on a turning machine that operates with different cutting speeds. Test specimens which underwent different machining processes and of different surface finish were also studied. The results obtained demonstrate the feasibility of surface roughness measurement using the proposed method.
Methods of producing alkylated hydrocarbons from an in situ heat treatment process liquid
Roes, Augustinus Wilhelmus Maria [Houston, TX; Mo, Weijian [Sugar Land, TX; Muylle, Michel Serge Marie [Houston, TX; Mandema, Remco Hugo [Houston, TX; Nair, Vijay [Katy, TX
2009-09-01
A method for producing alkylated hydrocarbons is disclosed. Formation fluid is produced from a subsurface in situ heat treatment process. The formation fluid is separated to produce a liquid stream and a first gas stream. The first gas stream includes olefins. The liquid stream is fractionated to produce at least a second gas stream including hydrocarbons having a carbon number of at least 3. The first gas stream and the second gas stream are introduced into an alkylation unit to produce alkylated hydrocarbons. At least a portion of the olefins in the first gas stream enhance alkylation.
In-situ neutron diffraction during biaxial deformation
Van Petegem, S.; Wagner, J.; Panzner, T.; Upadhyay, M.V.; Trang, T.T.T.; Van Swygenhoven, H.
2016-01-01
A change in strain path may have a significant effect on the mechanical response of metals. In order to understand or even predict the macroscopic behaviour under such conditions a detailed knowledge on the microstructural evolution is crucial. Yet relatively little work has been done to quantify and understand how the inter- and intragranular strains are affected during a change in strain path. In this work we present a new multiaxial deformation rig that allows performing in situ proportion...
NewIn-situ synthesis method of magnesium matrix composites reinforced with TiC particulates
Directory of Open Access Journals (Sweden)
Zhang Xiuqing
2006-12-01
Full Text Available Magnesium matrix composites reinforced with TiC particulates was prepared using a new in-situ synthesis method of remelting and dilution technique. And measurements were performed on the composites. The results of x ray diffraction (XRD analysis confirmed that TiC particulates were synthesized during the sintering process, and they retained in magnesium matrix composites after the remelting and dilution processing. From the microstructure characterization and electron probe microanalysis (EPMA, we could see that fine TiC particulates distributed uniformly in the matrix material.
A whole-mount in situ hybridization method for microRNA detection in Caenorhabditis elegans.
Andachi, Yoshiki; Kohara, Yuji
2016-07-01
Whole-mount in situ hybridization (WISH) is an outstanding method to decipher the spatiotemporal expression patterns of microRNAs (miRNAs) and provides important clues for elucidating their functions. The first WISH method for miRNA detection was developed in zebrafish. Although this method was quickly adapted for other vertebrates and fruit flies, WISH analysis has not been successfully used to detect miRNAs in Caenorhabditis elegans Here, we show a novel WISH method for miRNA detection in C. elegans Using this method, mir-1 miRNA was detected in the body-wall muscle where the expression and roles of mir-1 miRNA have been previously elucidated. Application of the method to let-7 family miRNAs, let-7, mir-48, mir-84, and mir-241, revealed their distinct but partially overlapping expression patterns, indicating that miRNAs sharing a short common sequence were distinguishably detected. In pash-1 mutants that were depleted of mature miRNAs, signals of mir-48 miRNA were greatly reduced, suggesting that mature miRNAs were detected by the method. These results demonstrate the validity of WISH to detect mature miRNAs in C. elegans. © 2016 Andachi and Kohara; Published by Cold Spring Harbor Laboratory Press for the RNA Society.
In-Situ Burning of Crude Oil on Water
DEFF Research Database (Denmark)
van Gelderen, Laurens
in the small scale water basin. Boilovers were also observed during the burning of a heavy crude oil with a substantial light fraction without a water layer, however, which suggests that water is not essential for boilover occurrence. Further studies are required to determine the conditions under which......The fire dynamics and fire chemistry of in-situ burning of crude oil on water was studied in order to improve predictions on the suitability of this oil spill response method. For this purpose, several operational parameters were studied to determine the factors that control the burning efficiency...... of in-situ burning, i.e. the amount of oil (in wt%) removed from the water surface by the burning process. The burning efficiency is the main parameter for expressing the oil removal effectiveness of in-situ burning as response method and is thus relevant for suitability predictions of in-situ burning...
In situ synthesis of protein arrays.
He, Mingyue; Stoevesandt, Oda; Taussig, Michael J
2008-02-01
In situ or on-chip protein array methods use cell free expression systems to produce proteins directly onto an immobilising surface from co-distributed or pre-arrayed DNA or RNA, enabling protein arrays to be created on demand. These methods address three issues in protein array technology: (i) efficient protein expression and availability, (ii) functional protein immobilisation and purification in a single step and (iii) protein on-chip stability over time. By simultaneously expressing and immobilising many proteins in parallel on the chip surface, the laborious and often costly processes of DNA cloning, expression and separate protein purification are avoided. Recently employed methods reviewed are PISA (protein in situ array) and NAPPA (nucleic acid programmable protein array) from DNA and puromycin-mediated immobilisation from mRNA.
Lan, Ru; Lin, Hai; Qiao, Bing; Dong, Yingbo; Zhang, Wei; Chang, Wen
2018-02-01
In this paper, the restoration behaviour of the in-situ microorganisms in seawater and sediments to the marine accident oil spill was researched. The experimental study on the breeding of in-situ petroleum-degrading bacteria in the seawater and sediments of Hangzhou Bay and the restoration of oil spill were carried out. Making use of the reinforced microbial flora, combined with physical and chemical methods in field environment, petroleum degrading and restoration experiment were performed, the effect of the breeding of in-situ degrading bacteria was evaluated, and the standard process of in-situ bacteria sampling, laboratory screening, domestication and degradation efficiency testing were formed. This study laid a foundation for further evaluation of the advantages and disadvantages for the petroleum-degrading bacteria of Hangzhou Bay during the process of in-situ restoration. The results showed that in-situ microbes of Hangzhou Bay could reach the growth peak in 5 days with the suitable environmental factors and sufficient nutrient elements, and the degradation efficiency could reach 65.2% (or 74.8% after acclimation). And also the microbes could adapt to the local sea water and environmental conditions, with a certain degree of degradation. The research results could provide parameter support for causal judgment and quantitative assessment of oil spill damage.
In-situ-gamma ray spectrometry for measurements of environmental radioactivity
Energy Technology Data Exchange (ETDEWEB)
Winkelmann, I
1994-12-31
A detailed description of the method is presented. The range of application is shown. The calibration of the in-situ gamma ray spectrometer with a HPGe semiconductor detector and the evaluation of the spectra are described. A measuring time of about 15-30 min is sufficient to determine the specific natural and man-made radioactivity of the soil of some ten Bq/m{sup 2}. The results of soil contamination measurements in Germany after the Chernobyl accident are reported. A total of 22 nuclides are detected. The measured contamination for the first days after the accident was as follows: {sup 132}Te/{sup 132}I - 100 kBq/m{sup 2}, and {sup 131}I - 70 kBq/m{sup 2}. 6 figs., 4 tabs. (orig.).
Evaluation and analysis of geological condition of in-situ fragmentation leaching uranium
International Nuclear Information System (INIS)
Yang Jianming; Tan Kaixuan; Huang Xiaonai
2003-01-01
The ore geological condition, hydrogeological condition, engineering geological condition and technological mineralogical character of in-situ fragmentation leaching uranium are analyzed, and it is considered that the implementation of in-situ fragmentation leaching uranium technology is decided by different geological factor. Previously prospecting and geological condition evaluation of uranium ore is based on traditional mining method. If in-situ fragmentation leaching uranium method is adopted, one must re-evaluate previously prospected deposits before they are mined, or one must evaluate new prospecting deposits according to geological conditions of in-situ fragmentation leaching uranium method. The feasibility evaluation method of uranium deposit by in-situ fragmentation leaching uranium put forward by B. N. Mociniets is introducd, and it is considered that B. N. Mociniets method has guidable significance for geological condition evaluation before uranium deposits are mined. A feasibility study is done by applying B. N. Mociniets method to a uranium deposit. (authors)
Quantitative in situ magnetization reversal studies in Lorentz microscopy and electron holography
International Nuclear Information System (INIS)
Rodríguez, L.A.; Magén, C.; Snoeck, E.; Gatel, C.; Marín, L.; Serrano-Ramón, L.
2013-01-01
A generalized procedure for the in situ application of magnetic fields by means of the excitation of the objective lens for magnetic imaging experiments in Lorentz microscopy and electron holography is quantitatively described. A protocol for applying magnetic fields with arbitrary in-plane magnitude and orientation is presented, and a freeware script for Digital Micrograph ™ is provided to assist the operation of the microscope. Moreover, a method to accurately reconstruct hysteresis loops is detailed. We show that the out-of-plane component of the magnetic field cannot be always neglected when performing quantitative measurements of the local magnetization. Several examples are shown to demonstrate the accuracy and functionality of the methods. - Highlights: • Generalized procedure for application of magnetic fields with the TEM objective lens. • Arbitrary in-plane magnetic field magnitude and orientation can be applied. • Method to accurately reconstruct hysteresis loops by electron holography. • Out-of-plane field component should be considered in quantitative measurements. • Examples to illustrate the method in Lorentz microscopy and electron holography
Wu, Madeline; Davidson, Norman
1981-01-01
A transmission electron microscope method for gene mapping by in situ hybridization to Drosophila polytene chromosomes has been developed. As electron-opaque labels, we use colloidal gold spheres having a diameter of 25 nm. The spheres are coated with a layer of protein to which Escherichia coli single-stranded DNA is photochemically crosslinked. Poly(dT) tails are added to the 3' OH ends of these DNA strands, and poly(dA) tails are added to the 3' OH ends of a fragmented cloned Drosophila DN...
Deka, Bhaskar Jyoti
2018-05-10
This study demonstrates the application of in situ ferrate (Fe(VI)) for the efficient removal of dissolved algal organic matter (AOM) from seawater. Sodium hypochlorite (NaOCl) and ferric (Fe(III)) were used to produce in situ Fe(VI) by wet chemical oxidation. First, the removal efficiencies of model AOM compounds, humic acid (HA), and sodium alginate (SA) were evaluated in the presence of sodium chloride with an initial influent dissolved organic carbon (DOC) concentration of 5.0 mg C L−1 at different pH levels to establish the optimal doses for in situ Fe(VI) generation. The concentration of Fe(VI) was determined by the 2,2-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) ultraviolet–visible spectrophotometry method. In the case of HA, 72% DOC removal was recorded when applied with 1.5 mg L−1 of Fe(III) and 1.5 mg L−1 of NaOCl (in situ Fe(VI) concentration of 1.46 mg L−1) while 42% DOC removal was observed for SA. Subsequently, the removal of AOM extracted from two bloom-forming algal species, Chaetoceros affinis (CA) and Hymenomonas (Hym), cultivated in seawater from the Red Sea, were tested with in situ generated Fe(VI) at the established optimum condition. In situ Fe(VI) recorded superior performance in removing AOM extracted from CA and Hym, showing 83% and 92% DOC removal when the influent DOC concentrations were 2.48 and 2.63 mg L−1, respectively. A detailed AOM characterization was conducted using liquid chromatography–organic carbon detection.
An in situ method for real-time monitoring of soil gas diffusivity
Laemmel, Thomas; Maier, Martin; Schack-Kirchner, Helmer; Lang, Friederike
2016-04-01
Soil aeration is an important factor for the biogeochemistry of soils. Generally, gas exchange between soil and atmosphere is assumed to be governed by molecular diffusion and by this way fluxes can be calculated using by Fick's Law. The soil gas diffusion coefficient DS represents the proportional factor between the gas flux and the gas concentration gradient in the soil and reflects the ability of the soil to "transport passively" gas through the soil. One common way to determine DS is taking core samples in the field and measuring DS in the lab. Unfortunately this method is destructive and laborious and it can only reflect a small fraction of the whole soil. As a consequence, uncertainty about the resulting effective diffusivity on the profile scale, i.e. the real aeration status remains. We developed a method to measure and monitor DS in situ. The set-up consists of a custom made gas sampling device, the continuous injection of an inert tracer gas and inverse gas transport modelling in the soil. The gas sampling device has seven sampling depths (from 0 to -43 cm of depth) and can be easily installed into vertical holes drilled by an auger, which allows for fast installation of the system. Helium (He) as inert tracer gas was injected continuously at the lower end of the device. The resulting steady state distribution of He was used to deduce the DS depth distribution of the soil. For Finite Element Modeling of the gas-sampling-device/soil system the program COMSOL was used. We tested our new method both in the lab and in a field study and compared the results with a reference lab method using soil cores. DS profiles obtained by our in-situ method were consistent with DS profiles determined based on soil core analyses. Soil gas profiles could be measured with a temporal resolution of 30 minutes. During the field study, there was an important rain event and we could monitor the decrease in soil gas diffusivity in the top soil due to water infiltration. The effect
Ninomiya, Kazuaki; Yamada, Ryuji; Matsumoto, Masami; Fukiya, Satoru; Katayama, Takane; Ogino, Chiaki; Shimizu, Nobuaki
2013-02-01
An image analyzing method was developed to evaluate in situ bioluminescence expression, without exposing the culture sample to the ambient oxygen atmosphere. Using this method, we investigated the effect of dissolved oxygen concentration on bioluminescence from an obligate anaerobe Bifidobacterium longum expressing bacterial luciferase which catalyzes an oxygen-requiring bioluminescent reaction. Copyright © 2012 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.
International Nuclear Information System (INIS)
Helbrecht, R.A.
2002-01-01
A scoping study was conducted over the period 1998-2000 to consider the feasibility of in situ disposal as a decommissioning option for AECL's Nuclear Power Demonstration Reactor located at Rolphton, Ontario. The results of a detailed assessment are summarized and the study concludes that in situ disposal appears feasible. Additional work required to confirm the results is also identified. A second in situ component, contaminated Winnipeg River sediments at AECL's Whiteshell Laboratory located in Manitoba, was also evaluated. That study concluded that in situ abandonment would have no adverse impact on aquatic life, humans and the environment. A summary of the study is presented as an appendix to the report. (author)
International Nuclear Information System (INIS)
Blalock, T.V.; Shepard, R.L.
1983-06-01
Methods for in situ calibration of reactor plant platinum resistance thermometers using Johnson noise measurements were tested in the laboratory and in two operating reactors: Diablo Canyon and Sequoyah. The Johnson noise methods provide an absolute measurement of the thermometer temperature and can be compared with the dc calibration of the thermometers to confirm their calibration without removing the thermometers from the plant coolant loops. Inaccuracies of less than 0.1% were obtained with these methods for ideal situations where the noise measuring equipment could be connected directly to the installed thermometer terminals. For measurements made at the ends of long extension cables, inaccuracies were 0.5 to 1.0%, at best. Extension cables must be optimized and well characterized electrically to achieve such accuracies. Other factors that affect the accuracy of these methods were evaluated
Process defects and in situ monitoring methods in metal powder bed fusion: a review
International Nuclear Information System (INIS)
Grasso, Marco; Colosimo, Bianca Maria
2017-01-01
Despite continuous technological enhancements of metal Additive Manufacturing (AM) systems, the lack of process repeatability and stability still represents a barrier for the industrial breakthrough. The most relevant metal AM applications currently involve industrial sectors (e.g. aerospace and bio-medical) where defects avoidance is fundamental. Because of this, there is the need to develop novel in situ monitoring tools able to keep under control the stability of the process on a layer-by-layer basis, and to detect the onset of defects as soon as possible. On the one hand, AM systems must be equipped with in situ sensing devices able to measure relevant quantities during the process, a.k.a. process signatures. On the other hand, in-process data analytics and statistical monitoring techniques are required to detect and localize the defects in an automated way. This paper reviews the literature and the commercial tools for in situ monitoring of powder bed fusion (PBF) processes. It explores the different categories of defects and their main causes, the most relevant process signatures and the in situ sensing approaches proposed so far. Particular attention is devoted to the development of automated defect detection rules and the study of process control strategies, which represent two critical fields for the development of future smart PBF systems. (paper)
Process defects and in situ monitoring methods in metal powder bed fusion: a review
Grasso, Marco; Colosimo, Bianca Maria
2017-04-01
Despite continuous technological enhancements of metal Additive Manufacturing (AM) systems, the lack of process repeatability and stability still represents a barrier for the industrial breakthrough. The most relevant metal AM applications currently involve industrial sectors (e.g. aerospace and bio-medical) where defects avoidance is fundamental. Because of this, there is the need to develop novel in situ monitoring tools able to keep under control the stability of the process on a layer-by-layer basis, and to detect the onset of defects as soon as possible. On the one hand, AM systems must be equipped with in situ sensing devices able to measure relevant quantities during the process, a.k.a. process signatures. On the other hand, in-process data analytics and statistical monitoring techniques are required to detect and localize the defects in an automated way. This paper reviews the literature and the commercial tools for in situ monitoring of powder bed fusion (PBF) processes. It explores the different categories of defects and their main causes, the most relevant process signatures and the in situ sensing approaches proposed so far. Particular attention is devoted to the development of automated defect detection rules and the study of process control strategies, which represent two critical fields for the development of future smart PBF systems.
Directory of Open Access Journals (Sweden)
Alexandre Zougman
Full Text Available Recently we introduced the concept of Suspension Trapping (STrap for bottom-up proteomics sample processing that is based upon SDS-mediated protein extraction, swift detergent removal and rapid reactor-type protein digestion in a quartz depth filter trap. As the depth filter surface is made of silica, it is readily modifiable with various functional groups using the silane coupling chemistries. Thus, during the digest, peptides possessing specific features could be targeted for enrichment by the functionalized depth filter material while non-targeted peptides could be collected as an unbound distinct fraction after the digest. In the example presented here the quartz depth filter surface is functionalized with the pyridyldithiol group therefore enabling reversible in-situ capture of the cysteine-containing peptides generated during the STrap-based digest. The described C-STrap method retains all advantages of the original STrap methodology and provides robust foundation for the conception of the targeted in-situ peptide fractionation in the STrap format for bottom-up proteomics. The presented data support the method's use in qualitative and semi-quantitative proteomics experiments.
Zougman, Alexandre; Banks, Rosamonde E
2015-01-01
Recently we introduced the concept of Suspension Trapping (STrap) for bottom-up proteomics sample processing that is based upon SDS-mediated protein extraction, swift detergent removal and rapid reactor-type protein digestion in a quartz depth filter trap. As the depth filter surface is made of silica, it is readily modifiable with various functional groups using the silane coupling chemistries. Thus, during the digest, peptides possessing specific features could be targeted for enrichment by the functionalized depth filter material while non-targeted peptides could be collected as an unbound distinct fraction after the digest. In the example presented here the quartz depth filter surface is functionalized with the pyridyldithiol group therefore enabling reversible in-situ capture of the cysteine-containing peptides generated during the STrap-based digest. The described C-STrap method retains all advantages of the original STrap methodology and provides robust foundation for the conception of the targeted in-situ peptide fractionation in the STrap format for bottom-up proteomics. The presented data support the method's use in qualitative and semi-quantitative proteomics experiments.
Energy Technology Data Exchange (ETDEWEB)
Kim, Tae Ho; Choi, Kyoung Joon; Yoo, Seung Chang; Kim, Ji Hyun [UNIST, Ulsan (Korea, Republic of)
2016-05-15
The two layered oxide structure is formed in pre-transition oxide for the zirconium alloy in high temperature water environment. It is known that the corrosion rate is related to the volume fraction of zirconium oxide and the pores in the oxides; therefore, the aim of this paper is to investigate the oxidation behavior in the pretransition zirconium oxide in high-temperature water chemistry. In this work, Raman spectroscopy was used for in situ investigations for characterizing the phase of zirconium oxide. In situ Raman spectroscopy is a well-suited technique for investigating in detail the characteristics of oxide films in a high-temperature corrosion environment. In previous studies, an in situ Raman system was developed for investigating the oxides on nickel-based alloys and low alloy steels in high-temperature water environment. Also, the early stage oxidation behavior of zirconium alloy with different dissolved hydrogen concentration environments in high temperature water was treated in the authors' previous study. In this study, a specific zirconium alloy was oxidized and investigated with in situ Raman spectroscopy for 100 d oxidation, which is close to the first transition time of the zirconium alloy oxidation. The ex situ investigation methods such as transmission electron microscopy (TEM) and energy dispersive X-ray spectroscopy (EDS) were used to further characterize the zirconium oxide structure. As oxidation time increased, the Raman peaks of tetragonal zirconium oxide were merged or became weaker. However, the monoclinic zirconium oxide peaks became distinct. The tetragonal zirconium oxide was just found near the O/M interface and this could explain the Raman spectra difference between the 30 d result and others.
Application of the nuclear x-ray fluorescence method to prospecting for gold in-situ
International Nuclear Information System (INIS)
Zhang, Y.; Xie, T.; Zhou, S.; Ge, L.
1989-01-01
Arsenic and chalcophile elements are often associated with gold, and can be considered indicator elements when prospecting for gold deposits. The nuclear geophysics X-ray fluorescence method can be used to search for hidden gold deposits by measuring fluorescence intensities of the indicator elements in situ. The method can speed geologic investigation and reduce exploration cost. Three types of portable radioisotope X-ray fluorescence analyzers, designed and manufactured by Chengdu College of Geology and Chongqing Geological Instrument Factory, are briefly introduced. These analyzers are widely used in different stages of geologic investigation for gold in China. In the two case histories presented five anomalous zones of X-ray fluorescence intensity related to gold mineralization are located and one hidden gold deposit is discovered with gold content of 23 g/t
International Nuclear Information System (INIS)
Zombori, P.; Andrasi, A.; Nemeth, I.
1992-06-01
A method was searched for to estimate the penetration characteristics of fallout radioactivity, using only spectral information obtained by in situ spectrometric measurements, and avoiding the need for long and tiresome sampling and sample analysis procedures. To speed up the analysis for depth distribution determination of fallout radioactivity in soil, an instrumental method based on the shape of spectra was developed. The ratio of peak to valley (the region between the photopeak and Compton edge) depends on the penetration of radionuclides in soil, providing an estimation of depth profile. These ratios were calculated and the method was tested by actual measurements. (R.P.) 7 refs.; 14 figs.; 2 tabs
In Situ Coupling of Ultrasound to Electro- and Photo-Deposition Methods for Materials Synthesis
Directory of Open Access Journals (Sweden)
Agnieszka Magdziarz
2017-01-01
Full Text Available This short review provides the current state-of-the-art of in situ coupling of ultrasound to chemical deposition methods. A synergetic action of the ultrasound and light radiation or electrical fields may result in new powerful methodologies, and these include sonophotodeposition and sonoelectrodeposition processes. The effect of ultrasound is explained on the base of different physical mechanisms emerging from cavitation phenomenon. Some possible mechanisms of the interactions between ultrasound and photochemical and electrochemical processes are discussed here. The application of sonophotodeposition and sonoelectrodeposition as green energy sources in the syntheses of different nanomaterials is also reviewed.
Zhang, Fang; Zhou, Ling-Xi; Liu, Li-Xin; Fang, Shuang-Xi; Yao, Bo; Xu, Lin; Zhang, Xiao-Chun; Masarie, Kenneth A; Conway, Thomas J; Worthy, Douglas E J; Ernst, Michele
2010-10-01
To strengthen scientific management and sharing of greenhouse gas data obtained from atmospheric background stations in China, it is important to ensure the standardization of observations and establish the data treatment and quality control procedure so as to maintain consistency in atmospheric carbon dioxide (CO2) and methane (CH4) measurements from different background stations. An automated gas chromatographic system (Hewlett Packard 5890GC employing flame ionization detection) for in situ measurements of atmospheric CO2 and CH4 has been developed since 1994 at the China Global Atmosphere Watch Baseline Observatory at Mt. Waliguan, in Qinhai. In this study, processing and quality control flow of CO2 and CH4 data acquired by HP ChemStation are discussed in detail, including raw data acquisition, data merge, time series inspection, operator flag, principal investigator flag, and the comparison of the GC measurement with the flask method. Atmosphere CO2 and CH4 mixing ratios were separated as background and non-background data using a robust local regression method, approximately 72% and 44% observed values had been filtered as background data for CO2 and CH4, respectively. Comparison of the CO1 and CH, in situ data to the flask sampling data were in good agreement, the relative deviations are within +/- 0.5% for CO2 and for CH4. The data has been assimilated into global database (Globalview-CO2, Globalview-CH4), submitted to the World Data Centre for Greenhouse Gases (WDCGG), and applied to World Meteorological Organization (WMO) Greenhouse Gas Bulletin and assessment reports of the United Nations Intergovernmental Panel on Climate Change (IPCC).
Jonge, de L.H.; Laar, van H.; Dijkstra, J.
2015-01-01
The in situ degradation of the washout fraction of starch in six feed ingredients (i.e. barley, faba beans, maize, oats, peas and wheat) was studied by using a modified in situ protocol and in vitro measurements. In comparison with the washing machine method, the modified protocol comprises a milder
Chattanooga shale: uranium recovery by in situ processing
International Nuclear Information System (INIS)
Jackson, D.D.
1977-01-01
The increasing demand for uranium as reactor fuel requires the addition of sizable new domestic reserves. One of the largest potential sources of low-grade uranium ore is the Chattanooga shale--a formation in Tennessee and neighboring states that has not been mined conventionally because it is expensive and environmentally disadvantageous to do so. An in situ process, on the other hand, might be used to extract uranium from this formation without the attendant problems of conventional mining. We have suggested developing such a process, in which fracturing, retorting, and pressure leaching might be used to extract the uranium. The potential advantages of such a process are that capital investment would be reduced, handling and disposing of the ore would be avoided, and leaching reagents would be self-generated from air and water. If successful, the cost reductions from these factors could make the uranium produced competitive with that from other sources, and substantially increase domestic reserves. A technical program to evaluate the processing problems has been outlined and a conceptual model of the extraction process has been developed. Preliminary cost estimates have been made, although it is recognized that their validity depends on how successfully the various processing steps are carried out. In view of the preliminary nature of this survey (and our growing need for uranium), we have urged a more detailed study on the feasibility of in situ methods for extracting uranium from the Chattanooga shale
He, Yongrui; Zhao, Xian-En; Wang, Renjun; Wei, Na; Sun, Jing; Dang, Jun; Chen, Guang; Liu, Zhiqiang; Zhu, Shuyun; You, Jinmao
2016-11-02
A simple, rapid, sensitive, selective, and environmentally friendly method, based on in situ derivatization ultrasound-assisted dispersive liquid-liquid microextraction (in situ DUADLLME) coupled with ultra-high-performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) using multiple reaction monitoring (MRM) mode has been developed for the simultaneous determination of food-related biogenic amines and amino acids. A new mass-spectrometry-sensitive derivatization reagent 4'-carbonyl chloride rosamine (CCR) was designed, synthesized, and first reported. Parameters and conditions of in situ DUADLLME and UHPLC-MS/MS were optimized in detail. Under the optimized conditions, the in situ DUADLLME was completed speedily (within 1 min) with high derivatization efficiencies (≥98.5%). With the cleanup and concentration of microextraction step, good analytical performance was obtained for the analytes. The results showed that this method was accurate and practical for quantification of biogenic amines and amino acids in common food samples (red wine, beer, wine, cheese, sausage, and fish).
Simulation of salt behavior using in situ response
International Nuclear Information System (INIS)
Li, W.T.
1986-01-01
The time-dependent nonlinear structural behavior in a salt formation around the openings can be obtained by either performing computational analysis of measuring in situ responses. However, analysis using laboratory test data may often deviate from the actual in situ conditions and geomechanical instruments can provide information only up to the time when the measurements were taken. A method has been suggested for simulating the salt behavior by utilizing the steady-state portion of in situ response history. Governing equations for computational analysis were normalized to the creep constant, the equations were solved, and the analytical response history was then computed in terms of normalized time. By synchronizing the response history obtained from the analysis to the one measured at the site, the creep constant was determined. Then the structural response of the salt was computed. This paper presents an improved method for simulating the salt behavior. In this method, the governing equations are normalized to the creep function, which represents the transient and the steady-state creep behavior. Both the transient and the steady-state portions of in situ response history are used in determining the creep function. Finally, a nonlinear mapping process relating the normalized and real time domains determines the behavior of the salt
In-situ polymerization PLOT columns I: divinylbenzene
Shen, T. C.
1992-01-01
A novel method for preparation of porous-layer open-tubular (PLOT) columns is described. The method involves a simple and reproducible, straight-forward in-situ polymerization of monomer directly on the metal tube.
Development of the integrated in situ Lasagna process
International Nuclear Information System (INIS)
Ho, S.; Athmer, C.; Sheridan, P.
1995-01-01
Contamination in deep, low permeability soils poses a significant technical challenge to in-situ remediation efforts. Poor accessibility to the contaminants and difficulty in uniform delivery of treatment reagents have rendered existing in-situ methods such as bioremediation, vapor extraction, and pump and treat rather ineffective when applied to low permeability soils present at many contaminated sites
DEFF Research Database (Denmark)
Valentin, Jan B.; Andreetta, Christian; Boomsma, Wouter
2014-01-01
We propose a method to formulate probabilistic models of protein structure in atomic detail, for a given amino acid sequence, based on Bayesian principles, while retaining a close link to physics. We start from two previously developed probabilistic models of protein structure on a local length s....... The results indicate that the proposed method and the probabilistic models show considerable promise for probabilistic protein structure prediction and related applications. © 2013 Wiley Periodicals, Inc....
Radiological visualization of prosthetic heart valves in situ
International Nuclear Information System (INIS)
Hoffmeister, H.M.; Pirschel, J.
1986-01-01
To determine the radiographic appearance of prosthetic heart valves 15 different models were investigated in situ. Fluoroscopy with detail radiographs and standard chest-radiographs were used for identification of the specific type of the prosthesis, for determination of details of the valve apparatus and for evaluation of the motion of the valve disc/ball. - Fluoroscopy and routine radiographs provided sufficient information to identify all prosthetic heart valves with exception of the Xenomedica bioprosthesis. If radiopaque material was used for the valve disc/ball, fluoroscopic assessment of the function of the valve was possible. Thus, in most types of disc or ball valves the opening/closing of the valve can be visualized, whereas in bioprosthesis a radiological determination of the mechanical function is not possible. (orig.) [de
Development of an in situ polymeric hydrogel implant of ...
African Journals Online (AJOL)
Purpose: To prepare and characterize in situ gel-forming implants of methylprednisolone for the treatment of spinal cord injuries. Methods: In situ hydrogels of methylprednisolone were prepared by dispersing polylactide glycolic acid (PLGA) polymer and methylprednisolone in N-methyl-pyrrolidone solvent, and subsequent ...
International Nuclear Information System (INIS)
Matsui, Hiroya
2005-09-01
This report describes for preliminary research plan in Phase 2 and Phase 3 taken into consideration of expected geological environment at location of URL based on the results of the investigations until FY 2003/2004. Duration of construction phase and total cost are considered as important factors for planning as well. The below items are planned for in-situ experiments in Phase 2 and Phase 3 in/around URL are planning. Phase 2. (In-situ experiments for understanding of geological environment) Geological survey at tunnel. Inflow measurement in shafts. Water pressure monitoring and groundwater sampling around shafts during excavation of URL. Investigation for EDZ around shafts. Stress measurement on support. Detail investigations for geological environment around drifts. Excavation disturbance experiment in a drift. Investigation for desaturation zone and REDOX condition around drifts. (Engineered barrier system) In-situ experiment on low-alkali concrete. In-situ experiment for gas migration in engineering barrier system. Phase 3. (In-situ experiments for understanding of geological environment) EDZ experiment for stress interference. Investigation of long-term behavior of EDZ around drifts. Detail investigation on fault/fault zone. Monitoring for the change of geological environment at earthquake. Backfill test in boreholes. (Engineered barrier system) T-H-M-C experiment. In-situ experiment for corrosion of overpack. Investigation of the influence of a concrete to engineering barrier system and geological environment. In-situ experiment for interference between backfill material and geological environment. Backfill test in a drift. (Safety assessment) Tracer tests in engineering barrier system, natural barrier and fault/fault zone. (author)
In-situ neutron diffraction during biaxial deformation
International Nuclear Information System (INIS)
Van Petegem, S.; Wagner, J.; Panzner, T.; Upadhyay, M.V.; Trang, T.T.T.; Van Swygenhoven, H.
2016-01-01
A change in strain path may have a significant effect on the mechanical response of metals. In order to understand or even predict the macroscopic behaviour under such conditions a detailed knowledge on the microstructural evolution is crucial. Yet relatively little work has been done to quantify and understand how the inter- and intragranular strains are affected during a change in strain path. In this work we present a new multiaxial deformation rig that allows performing in situ proportional and non-proportional loading under neutron diffraction. We demonstrate the capabilities of this new setup for the case of a 316 L stainless steel. We show that the nature and magnitude of intergranular strain strongly depends on the applied stress state and demonstrate that micro yielding and internal strain recovery are responsible for the observed transient softening during a 90° strain path change. We anticipate that this new characterization method will provide previously inaccessible microstructural data that can serve as input for benchmarking current state-of-the-art crystal plasticity models.
International Nuclear Information System (INIS)
Fay, P.J.; Ray, J.R.; Wolf, R.J.
1994-01-01
We present a new, nondestructive, method for determining chemical potentials in Monte Carlo and molecular dynamics simulations. The method estimates a value for the chemical potential such that one has a balance between fictitious successful creation and destruction trials in which the Monte Carlo method is used to determine success or failure of the creation/destruction attempts; we thus call the method a detailed balance method. The method allows one to obtain estimates of the chemical potential for a given species in any closed ensemble simulation; the closed ensemble is paired with a ''natural'' open ensemble for the purpose of obtaining creation and destruction probabilities. We present results for the Lennard-Jones system and also for an embedded atom model of liquid palladium, and compare to previous results in the literature for these two systems. We are able to obtain an accurate estimate of the chemical potential for the Lennard-Jones system at higher densities than reported in the literature
Ex-situ and in-situ mineral carbonation as a means to sequester carbon dioxide
Energy Technology Data Exchange (ETDEWEB)
Gerdemann, Stephen J.; Dahlin, David C.; O' Connor, William K.; Penner, Larry R.; Rush, G.E.
2004-01-01
The U. S. Department of Energy's Albany Research Center is investigating mineral carbonation as a method of sequestering CO2 from coal-fired-power plants. Magnesium-silicate minerals such as serpentine [Mg3Si2O5(OH)4] and olivine (Mg2SiO4) react with CO2 to produce magnesite (MgCO3), and the calcium-silicate mineral, wollastonite (CaSiO3), reacts to form calcite (CaCO3). It is possible to carry out these reactions either ex situ (above ground in a traditional chemical processing plant) or in situ (storage underground and subsequent reaction with the host rock to trap CO2 as carbonate minerals). For ex situ mineral carbonation to be economically attractive, the reaction must proceed quickly to near completion. The reaction rate is accelerated by raising the activity of CO2 in solution, heat (but not too much), reducing the particle size, high-intensity grinding to disrupt the crystal structure, and, in the case of serpentine, heat-treatment to remove the chemically bound water. All of these carry energy/economic penalties. An economic study illustrates the impact of mineral availability and process parameters on the cost of ex situ carbon sequestration. In situ carbonation offers economic advantages over ex situ processes, because no chemical plant is required. Knowledge gained from the ex situ work was applied to long-term experiments designed to simulate in situ CO2 storage conditions. The Columbia River Basalt Group (CRBG), a multi-layered basaltic lava formation, has potentially favorable mineralogy (up to 25% combined concentration of Ca, Fe2+, and Mg cations) for storage of CO2. However, more information about the interaction of CO2 with aquifers and the host rock is needed. Core samples from the CRBG, as well as samples of olivine, serpentine, and sandstone, were reacted in an autoclave for up to 2000 hours at elevated temperatures and pressures. Changes in core porosity, secondary mineralizations, and both solution and solid chemistry were measured.
Immediate assessment of in situ gas content using underground manometric desorbometer
Energy Technology Data Exchange (ETDEWEB)
Lunaezewski, L.W.; Mahoney, M.R. [Lunagas Pty. Ltd., Newcastle, NSW (Australia)
1995-12-31
An underground assessment of gas content (within 30 minutes) can be used as a complementary method for the immediate establishment of the magnitudes of in situ gas content at a distance of 5 to 6 metres ahead of a heading face. The indirect method of in situ gas content measurement, using a manometric desorbometer, can be used to establish the direct relationship between underground desorbometer readings and corresponding in situ gas content values for a selected coal seam. 3 refs., 3 figs.
Energy Technology Data Exchange (ETDEWEB)
Peulon, S.; Lacroix, A.; Chausse, A. [Univ. d' Evry-val-d' Essonne, Laboratoire Analyse et Modelisation pour la Biologie et l' Environnement (LAMBE CNRS UMR 8587), 91 - Evry (France); Larabi-Gruet, N. [CEA Saclay, Dept. de Physico-Chimie (DEN/DPC/SECR/L3MR), 91 - Gif sur Yvette (France)
2007-07-01
This work deals with the electrodeposition of manganese compounds. A systematic study of the synthesis experimental conditions has been carried out, and the obtained depositions have been characterized by different ex-situ analyses methods (XRD, FTIR, SEM). The in-situ measurements of mass increase with a quartz microbalance during the syntheses have allowed to estimate the growth mechanisms which are in agreement with the ex-situ characterizations. The cation has an important role in the nature of the electrodeposited compounds. In presence of sodium, a mixed lamellar compound Mn(III)/Mn(IV), the birnessite, is deposited, whereas in presence of potassium, bixbyite is formed (Mn{sub 2}O{sub 3}), these two compounds having a main role in the environment. The substrate can also influence the nature of the formed intermediary compounds. Little studied compounds such as feitkneichtite ({beta}-MnOOH) and groutite ({alpha}-MnOOH) have been revealed. (O.M.)
International Nuclear Information System (INIS)
Peulon, S.; Lacroix, A.; Chausse, A.; Larabi-Gruet, N.
2007-01-01
This work deals with the electrodeposition of manganese compounds. A systematic study of the synthesis experimental conditions has been carried out, and the obtained depositions have been characterized by different ex-situ analyses methods (XRD, FTIR, SEM). The in-situ measurements of mass increase with a quartz microbalance during the syntheses have allowed to estimate the growth mechanisms which are in agreement with the ex-situ characterizations. The cation has an important role in the nature of the electrodeposited compounds. In presence of sodium, a mixed lamellar compound Mn(III)/Mn(IV), the birnessite, is deposited, whereas in presence of potassium, bixbyite is formed (Mn 2 O 3 ), these two compounds having a main role in the environment. The substrate can also influence the nature of the formed intermediary compounds. Little studied compounds such as feitkneichtite (β-MnOOH) and groutite (α-MnOOH) have been revealed. (O.M.)
Method for determining detailed rod worth profiles at low power in the fast test reactor
International Nuclear Information System (INIS)
Sevenich, R.A.
1975-08-01
A method for obtaining a detailed rod worth profile at low power for a slow control rod insertion is presented. The accuracy of the method depends on a preparatory experiment in which the test rod is dropped quickly to yield, upon analysis, the magnitude of the rod worth and an effective source value. These numbers are employed to initialize the inverse kinetics analysis for the slow insertion. Corrections for changes in detection efficiency are not included for the simulated experiments. (U.S.)
Zhan, Fangfang; Zhou, Xiaoming
2012-12-01
Rotaviruses are double-stranded RNA viruses belonging to the family of enteric pathogens. It is a major cause of diarrhoeal disease in infants and young children worldwide. Consequently, rapid and accurate detection of rotaviruses is of great importance in controlling and preventing food- and waterborne diseases and outbreaks. Reverse transcription-polymerase chain reaction (RT-PCR) is a reliable method that possesses high specificity and sensitivity. It has been widely used to detection of viruses. Electrochemiluminescence (ECL) can be considered as an important and powerful tool in analytical and clinical application with high sensitivity, excellent specificity, and low cost. Here we have developed a method for the detection of rotavirus by combining in situ magnetic beads (MBs) based RT-PCR with ECL. RT of rotavirus RNA was carried out in a traditional way and the resulting cDNA was directly amplified on MBs. Forward primers were covalently bounded to MBs and reverse primers were labeled with tris-(2, 2'-bipyridyl) ruthenium (TBR). During the PCR cycling, the TBR labeled products were directly loaded and enriched on the surface of MBs. Then the MBs-TBR complexes could be analyzed by a magnetic ECL platform without any post-modification or post-incubation which avoid some laborious manual operations and achieve rapid yet sensitive detection. In this study, rotavirus from fecal specimens was successfully detected within 2 h, and the limit of detection was estimated to be 104copies/μL. This novel in situ MBs based RT-PCR with ECL detection method can be used for pathogen detection in food safety field and clinical diagnosis.
Energy Technology Data Exchange (ETDEWEB)
Dutta, A.; Sahir, A.; Tan, E.; Humbird, D.; Snowden-Swan, L. J.; Meyer, P.; Ross, J.; Sexton, D.; Yap, R.; Lukas, J.
2015-03-01
This report was developed as part of the U.S. Department of Energy’s Bioenergy Technologies Office’s efforts to enable the development of technologies for the production of infrastructurecompatible, cost-competitive liquid hydrocarbon fuels from biomass. Specifically, this report details two conceptual designs based on projected product yields and quality improvements via catalyst development and process integration. It is expected that these research improvements will be made within the 2022 timeframe. The two conversion pathways detailed are (1) in situ and (2) ex situ upgrading of vapors produced from the fast pyrolysis of biomass. While the base case conceptual designs and underlying assumptions outline performance metrics for feasibility, it should be noted that these are only two of many other possibilities in this area of research. Other promising process design options emerging from the research will be considered for future techno-economic analysis.
In situ solution mining technique
International Nuclear Information System (INIS)
Learmont, R.P.
1978-01-01
A method of in situ solution mining is disclosed in which a primary leaching process employing an array of 5-spot leaching patterns of production and injection wells is converted to a different pattern by converting to injection wells all the production wells in alternate rows
Energy Technology Data Exchange (ETDEWEB)
Muthmann, Stefan
2012-08-22
This work deals with the design and application of a novel experiment, which enables in-situ Raman measurements during the parallel plate plasma enhanced chemical vapor deposition (PECVD) of {mu}cSi:H. Measurements of the crystalline volume fraction (I{sub C}{sup RS}) and the temperature of a growing film are carried out using the novel setup. To enable in-situ Raman measurement of central regions of the coated substrate in a PECVD system, optical access under normal incidence is necessary. An experimental setup in which an optical feed-through was integrated into a PECVD electrode was developed. This setup introduces a disturbance to the electrical field which sustains the plasma. By designing metallic shields the impact of the feed through was reduced considerably at low optical losses. The homogeneity of films deposited with the novel setup in different growth regimes was studied. A correlation between the magnitude of the inhomogeneity caused by the feed-through and the characteristics of the deposition regimes is found. Raman spectroscopy demands the illumination of a sample with a laser and the collection of the scattered radiation. Due to absorption of the laser light the temperature of the illuminated film is increased. Since the temperature determines the properties of a growing film the laser-induced temperature increase was studied. By pulsing the laser radiation of minimal temperature increase at maximal signal intensity was obtained. The crystalline volume fraction of a growing {mu}cSi:H layer was determined in-situ with the novel setup. A minimal temporal resolution of less than 17.5 s at sufficient signal-to-noise-ratio was achieved, which corresponds to less than 9 nm of deposited material during one measurement interval at the industrial standard growth rate of 0.5 nm/s. The obtained results were compared to depth resolved measurements which were carried out after the deposition. An excellent agreement between both methods validates the reliability
Investigation on the Vibration Effect of Shock Wave in Rock Burst by In Situ Microseismic Monitoring
Directory of Open Access Journals (Sweden)
Mingshi Gao
2018-01-01
Full Text Available Rock burst is a physical explosion associated with enormous damage at a short time. Due to the complicity of mechanics of rock burst in coal mine roadway, the direct use of traditional investigation method applied in tunnel is inappropriate since the components of surrounding rock are much more complex in underground than that of tunnel. In addition, the reliability of the results obtained through these methods (i.e., physical simulation, theoretical analysis, and monitoring in filed application is still not certain with complex geological conditions. Against this background, present experimental study was first ever conducted at initial site to evaluate the effect of shock wave during the rock burst. TDS-6 microseismic monitoring system was set up in situ to evaluate the propagation of shock wave resulting in microexplosions of roadway surrounding rock. Various parameters including the distance of epicentre and the characteristic of response have been investigated. Detailed test results revealed that (1 the shock wave attenuated exponentially with the increase of the distance to seismic source according to the equation of E=E0e-ηl; particularly, the amplitude decreased significantly after being 20 m apart from explosive resource and then became very weak after being 30 m apart from the seismic source; (2 the response mechanics are characteristic with large scatter based on the real location of surrounding rock despite being at the same section. That is, the surrounding rock of floor experienced serious damage, followed by ribs, the roof, and the humeral angles. This in situ experimental study also demonstrated that microseismic monitoring system can be effectively used in rock burst through careful setup and data investigation. The proposed in situ monitoring method has provided a new way to predict rock burst due to its simple instalment procedure associated with direct and reasonable experimental results.
Meddings, Nina; Owen, John R.; Garcia-Araez, Nuria
2017-10-01
Lithium ion conducting membranes are important to protect the lithium metal electrode and act as a barrier to crossover species such as polysulphides in Li-S systems, redox mediators in Li-O2 cells or dissolved cathode species or electrolyte oxidation products in high voltage Li-ion batteries. We present an in-situ method for measuring permeability of membranes to crossover redox species. The method employs a 'Swagelok' cell design equipped with a glassy carbon working electrode, in which redox species are placed initially in the counter electrode compartment only. Permeability through the membrane, which separates working and counter electrodes, is determined using a square wave voltammetry technique that allows the concentration of crossover redox species to be evaluated over time with very high precision. We test the method using a model and well-behaved electrochemical system to demonstrate its sensitivity, reproducibility and reliability relative to alternative approaches. This new method offers advantages in terms of small electrolyte volume, and simple, fast, quantitative and in-situ measurement.
Determination of in-situ fracture apertures from digital borehole images
International Nuclear Information System (INIS)
Johansson, Maria C.; Stephansson, O.
1998-01-01
Imaging methods applied to borehole investigations have become common for mapping and characterisation of the rock mass. Today we have access to detailed information about the rock, but we lack some methods for analysis. In this study we develop a methodology for measurements of in-situ fracture geometry, from optical borehole images (BIP-system). We focus on the detailed information about fracture geometry, available thanks to the high image resolution. We have decided to perform the measurements using digital image processing, to avoid bias from the human analyst, and we present on-going work on the image processing methodology. Our method is based on iterative intensity thresholding. We work on grey-scale images, of open fractures that fully intersect the borehole. The fracture trace comes out as a dark sinusoidal in the borehole image. First, the darkest pixels in the image are extracted. Then the pixels, which are immediate neighbours to the first set, are included, under the condition that they are darker than a somewhat lower threshold. The including of neighbours is repeated until the fracture trace is filled. The resulting sinusoidal fracture trace is then used for finding an approximation of the fracture plane. The fracture plane orientation is used for determination of true aperture from the apparent aperture seen in the image. After this, fracture aperture statistics can be determined. The method works well for images of open fractures of simple geometry (sine wave). It needs to be improved to handle more complex geometry, e.g. crossing fracture traces. Today, some minor interaction from the analyst is needed, but slight modifications will minimise this
TSSM: The in situ exploration of Titan
Coustenis, A.; Lunine, J. I.; Lebreton, J. P.; Matson, D.; Reh, K.; Beauchamp, P.; Erd, C.
2008-09-01
probe will descend through Titan's atmosphere and land on a liquid surface (at the North pole, in a lake according to the current design). The currently envisaged strawman payload for these elements will be presented. Instruments aboard the balloon would provide high resolution vistas of the surface of Titan as the balloon cruises at 10 km altitude, as well as make compositional measurements of the surface, detailed sounding of crustal layering, and chemical measurements of aerosols. A magnetometer, unimpeded by Titan's ionosphere, would permit sensitive detection of induced or intrinsic fields. The short-lived probe would splash into a large northern sea and spend several hours floating during which direct chemical and physical sampling of the liquid—a carrier for many dissolved organic species— would be undertaken. During its descent the Mare Explorer would provide the first in situ profiling of the winter northern hemispheric atmosphere, which is distinctly different from the equatorial atmosphere where Huygens descended and the balloon will arrive. Coordinated radio science experiments aboard the orbiter and in situ elements would be capable of providing detailed information on Titan's tidal response, and hence its crustal rigidity and thickness.
In situ measurements of fuel retention by laser induced desorption spectroscopy in TEXTOR
Zlobinski, M.; Philipps, V.; Schweer, B.; Huber, A.; Stoschus, H.; Brezinsek, S.; Samm, U.; TEXTOR Team
2011-12-01
In future fusion devices such as ITER tritium retention due to tritium co-deposition in mixed material layers can be a serious safety problem. Laser induced desorption spectroscopy (LIDS) can measure the hydrogen content of hydrogenic carbon layers locally on plasma-facing components, while hydrogen is used as a tritium substitute. For several years, this method has been applied in the TEXTOR tokamak in situ during plasma operation to monitor the hydrogen content in space and time. This work shows the LIDS signal reproducibility and studies the effects of different plasma conditions, desorption distances from the plasma and different laser energies using a dedicated sample with constant hydrogen amount. Also the LIDS signal evaluation procedure is described in detail and the detection limits for different conditions in the TEXTOR tokamak are estimated.
In situ electron backscatter diffraction (EBSD) during the compression of micropillars
International Nuclear Information System (INIS)
Niederberger, C.; Mook, W.M.; Maeder, X.; Michler, J.
2010-01-01
For the first time, in situ electron backscatter diffraction (EBSD) measurements during compression experiments by a modified nanoindenter on micron-sized single crystal pillars are demonstrated here. The experimental setup and the requirements concerning the compression sample are described in detail. EBSD mappings have been acquired before loading, under load and after unloading for consecutive compression cycles on a focused ion beam (FIB) milled GaAs micropillar. In situ EBSD allows for the determination of crystallographic orientation with sub-100 nm spatial resolution. Thereby, it provides highly localized information pertaining to the deformation phenomena such as elastic bending of the micropillar or the formation of deformation twins and plastic orientation gradients due to geometrically necessary dislocations. The most striking features revealed by in situ EBSD are the non-negligible amount of reversible (elastic) bending of the micropillar and the fact that deformation twinning and dislocation glide initiate where the bending is strongest. Due to this high spatial and orientation resolution, in situ EBSD measurements during micromechanical testing are demonstrated to be a promising technique for the investigation of deformation phenomena at the nano- to micro-scale.
In-situ observation of structure formation in polymer processing
International Nuclear Information System (INIS)
Murase, Hiroki
2009-01-01
In-situ X-ray scattering in polymer processing is a crucial method to elucidate the mechanism of structure formation in the process. Fiber spinning is one such process primarily imposing extensional deformation on polymeric melt at the spin-line during rapid cooling. In-situ small-angle X-ray scattering using synchrotron radiation on the spinning process allows direct observation of the transient structure developing in the process. (author)
paediatric ureteric calculi: in-situ extracorporeal shock wave lithotripsy
African Journals Online (AJOL)
Objective To evaluate prospectively the efficacy of in-situ extracorporeal shock wave lithotripsy (ESWL) in the treatment of ureteric calculi in the paediatric age group. Patients and Methods Twenty children (aged 2.2 16 years) with 22 ureteric stones were evaluated and treated with in-situ ESWL using the Dornier S lithotripter ...
International Nuclear Information System (INIS)
Chhillar, Sumit; Acharya, R.; Sodaye, S.; Pujari, P.K.
2014-01-01
A particle induced gamma-ray emission (PIGE) method using proton beam has been standardized for determination of isotopic composition of natural boron and enriched boron samples. Target pellets of boron standard and samples were prepared in cellulose matrix. The prompt gamma rays of 429 keV, 718 keV and 2125 keV were measured from 10 B(p,αγ) 7 Be, 10 B(p, p'γ) 10 B and 11 B(p, p'γ) 11 B nuclear reactions, respectively. For normalizing the beam current variations in situ current normalization method was used. Validation of method was carried out using synthetic samples of boron carbide, borax, borazine and lithium metaborate in cellulose matrix. (author)
Stream temperature estimated in situ from thermal-infrared images: best estimate and uncertainty
International Nuclear Information System (INIS)
Iezzi, F; Todisco, M T
2015-01-01
The paper aims to show a technique to estimate in situ the stream temperature from thermal-infrared images deepening its best estimate and uncertainty. Stream temperature is an important indicator of water quality and nowadays its assessment is important particularly for thermal pollution monitoring in water bodies. Stream temperature changes are especially due to the anthropogenic heat input from urban wastewater and from water used as a coolant by power plants and industrial manufacturers. The stream temperatures assessment using ordinary techniques (e.g. appropriate thermometers) is limited by sparse sampling in space due to a spatial discretization necessarily punctual. Latest and most advanced techniques assess the stream temperature using thermal-infrared remote sensing based on thermal imagers placed usually on aircrafts or using satellite images. These techniques assess only the surface water temperature and they are suitable to detect the temperature of vast water bodies but do not allow a detailed and precise surface water temperature assessment in limited areas of the water body. The technique shown in this research is based on the assessment of thermal-infrared images obtained in situ via portable thermal imager. As in all thermographic techniques, also in this technique, it is possible to estimate only the surface water temperature. A stream with the presence of a discharge of urban wastewater is proposed as case study to validate the technique and to show its application limits. Since the technique analyzes limited areas in extension of the water body, it allows a detailed and precise assessment of the water temperature. In general, the punctual and average stream temperatures are respectively uncorrected and corrected. An appropriate statistical method that minimizes the errors in the average stream temperature is proposed. The correct measurement of this temperature through the assessment of thermal- infrared images obtained in situ via portable
Song, Biao; Zeng, Guangming; Gong, Jilai; Liang, Jie; Xu, Piao; Liu, Zhifeng; Zhang, Yi; Zhang, Chen; Cheng, Min; Liu, Yang; Ye, Shujing; Yi, Huan; Ren, Xiaoya
2017-08-01
Soil and sediment contamination has become a critical issue worldwide due to its great harm to the ecological environment and public health. In recent years, many remediation technologies including physical, chemical, biological, and combined methods have been proposed and adopted for the purpose of solving the problems of soil and sediment contamination. However, current research on evaluation methods for assessing these remediation technologies is scattered and lacks valid and integrated evaluation methods for assessing the remediation effectiveness. This paper provides a comprehensive review with an environmental perspective on the evaluation methods for assessing the effectiveness of in situ remediation of soil and sediment contaminated with organic pollutants and heavy metals. The review systematically summarizes recent exploration and attempts of the remediation effectiveness assessment based on the content of pollutants, soil and sediment characteristics, and ecological risks. Moreover, limitations and future research needs of the practical assessment are discussed. These limitations are not conducive to the implementation of the abatement and control programs for soil and sediment contamination. Therefore, more attention should be paid to the evaluation methods for assessing the remediation effectiveness while developing new in situ remediation technologies in future research. Copyright © 2017 Elsevier Ltd. All rights reserved.
In situ stress measurement with the new LVDT - Cell - method description and verification
International Nuclear Information System (INIS)
Hakala, M.; Christiansson, R.; Martin, D.; Siren, T.; Kemppainen, K.
2013-11-01
Posiva Oy and SKB (Svensk Kaernbraenslehantering AB) tested the suitability a new LVDT-cell (Linear Variable Differential Transducer cell) to measure the induced stresses in the vicinity of an excavated surface and further to use these results to interpret the in situ state of stress. It utilises the overcoring methodology, measuring the radial convergence of four diameters using eight LVDTs, and is similar in concept to the USBM-gauge. A 127 mm diameter pilot-hole is required and the overcore diameter is 200 mm. The minimum overcoring length is 350 mm, and hence a compact drill can be utilised. Extensive testing of the LVDT-cell shows it to be robust and suitable for use in an underground environment. Sensitivity tests also show that the cell can withstand a range of operating conditions and still provide acceptable results. The in situ stress at the measurement location can be solved by numerical inversion using the results of at least three overcoring measurements around the three-dimensional tunnel section. The large dimensions of the measurement tool and the ability to utilise multiple measurements at various locations in a tunnel section, provides flexibility in selecting an appropriate rock mass volume. Because the inversion technique relies on knowing the exact location of the measurements and the geometry profile of the tunnel, modern survey techniques such as Lidar or photogrammetric technology should be used. Checks using traditional surveying techniques should also be used to ensure adequate survey resolution, specially in case of sidecoring measurements. To evaluate the suitability of the LVDT-cell to provide the in situ state of stress, tests were carried out in the drill-and-blast TASS tunnel and TBM tunnel at the Aespoe Hard Rock Laboratory in Sweden. The state of stress established using the LVDT-cell was in agreement with the state of stress established previously using traditional overcoring and hydraulic fracturing methods. In this study, the
In situ stress measurement with the new LVDT - Cell - method description and verification
Energy Technology Data Exchange (ETDEWEB)
Hakala, M. [KMS Hakala Oy, Nokia (Finland); Christiansson, R. [Svensk Kaernbraenslehantering AB, Stockholm (Sweden); Martin, D. [Univ. of Alberta, Edmonton (Canada); Siren, T.; Kemppainen, K.
2013-11-15
Posiva Oy and SKB (Svensk Kaernbraenslehantering AB) tested the suitability a new LVDT-cell (Linear Variable Differential Transducer cell) to measure the induced stresses in the vicinity of an excavated surface and further to use these results to interpret the in situ state of stress. It utilises the overcoring methodology, measuring the radial convergence of four diameters using eight LVDTs, and is similar in concept to the USBM-gauge. A 127 mm diameter pilot-hole is required and the overcore diameter is 200 mm. The minimum overcoring length is 350 mm, and hence a compact drill can be utilised. Extensive testing of the LVDT-cell shows it to be robust and suitable for use in an underground environment. Sensitivity tests also show that the cell can withstand a range of operating conditions and still provide acceptable results. The in situ stress at the measurement location can be solved by numerical inversion using the results of at least three overcoring measurements around the three-dimensional tunnel section. The large dimensions of the measurement tool and the ability to utilise multiple measurements at various locations in a tunnel section, provides flexibility in selecting an appropriate rock mass volume. Because the inversion technique relies on knowing the exact location of the measurements and the geometry profile of the tunnel, modern survey techniques such as Lidar or photogrammetric technology should be used. Checks using traditional surveying techniques should also be used to ensure adequate survey resolution, specially in case of sidecoring measurements. To evaluate the suitability of the LVDT-cell to provide the in situ state of stress, tests were carried out in the drill-and-blast TASS tunnel and TBM tunnel at the Aespoe Hard Rock Laboratory in Sweden. The state of stress established using the LVDT-cell was in agreement with the state of stress established previously using traditional overcoring and hydraulic fracturing methods. In this study, the
Hongyang, Yu; Zhengang, Lu; Xi, Yang
2017-05-01
Modular Multilevel Converter is more and more widely used in high voltage DC transmission system and high power motor drive system. It is a major topological structure for high power AC-DC converter. Due to the large module number, the complex control algorithm, and the high power user’s back ground, the MMC model used for simulation should be as accurate as possible to simulate the details of how MMC works for the dynamic testing of the MMC controller. But so far, there is no sample simulation MMC model which can simulate the switching dynamic process. In this paper, one curve embedded full-bridge MMC modeling method with detailed representation of IGBT characteristics is proposed. This method is based on the switching curve referring and sample circuit calculation, and it is sample for implementation. Based on the simulation comparison test under Matlab/Simulink, the proposed method is proved to be correct.
Some technical details concerning a new method of gravimetric-seismic inversion
DEFF Research Database (Denmark)
Strykowski, Gabriel
1999-01-01
In this paper a number of technical details related to a new method of gravimetric-seismic inversion, which is still under development, are explained. Although the present contribution aims on providing general statements on how to formulate and solve complex gravimetric-seismic modeling; problems......, the inspiration comes from the practical modeling problems in the area of Jutland peninsula (Denmark). More specifically, the methodological aspects of the proposed inversion method are illustrated on a problem of 3D modeling of the intra crustal intrusion associated with the Silkeborg Gravity High. The existing...... refraction seismic profile locates the source of the anomaly in depths 10 km - 18 km. In an earlier publication, (Strykowski, 1998), and for the same test area, a method of complex geological stripping is described. The present contribution is a continuation of this paper in the direction of inversion...
The hands in metabolic skeletal diseases 1. A method of high-detailed contact radiography
International Nuclear Information System (INIS)
Shotemor, Sh.Sh.; Tret'yakov, A.E.
1982-01-01
A method of high-detailed contact radiography of the hands consists in the the screenless x-ray on the fine grained technical film employing microfocus with consecutive optical enlargement of the appearance. Various specimens of home-made tecinical film have been tried, the best results were obtained with the PT-5 film type (an opportunity of 7-fold optical magnification without interfering effect of the emulsion granularity). The method provides for a significant diagnostic advantage, affording to reveal minimal manifestations of subperiosteal, intracortical, and enosteal bone tissue resorption, as well as tiny calcinates in soft tissues. Pathological bone disorders were discovered in 63 out of 142 examined patients, suspected of metabolic diseases of the skeleton [ru
Detailed Design and Fabrication Method of the ITER Vacuum Vessel Ports
International Nuclear Information System (INIS)
Hee-Jae Ahn; Kwon, T.H.; Hong, Y.S.
2006-01-01
The engineering design of the ITER vacuum vessel (VV) has been progressed by the ITER International Team (IT) with the cooperation of several participant teams (PT). The VV and ports are the components allocated to Korea for the construction of the ITER. Hyundai Heavy Industries has been involved in the structural analysis, detailed design and development of the fabrication method of the upper and lower ports within the framework of the ITER transitional arrangements (ITA). The design of the port structures has been investigated to validate and to improve the conceptual designs of the ITER IT and other PT. The special emphasis was laid on the flange joint between the port extension and the in-port plug to develop the design of the upper port. The modified design with a pure friction type flange with forty-eight pieces of bolts instead of the tangential key is recommended. Furthermore, the alternative flange designs developed by the ITER IT have been analyzed in detail to simplify the lip seal maintenance into the port flange. The structural analyses of the lower RH port have been also performed to verify the capacity for supporting the VV. The maximum stress exceeds the allowable value at the reinforcing block and basement. More elaborate local models have been developed to mitigate the stress concentration and to modify the component design. The fabrication method and the sequence of the detailed fabrication for the ports are developed focusing on the cost reduction as well as the simplification. A typical port structure includes a port stub, a stub extension and a port extension with a connecting duct. The fabrication sequence consists of surface treatment, cutting, forming, cleaning, welding, machining, and non-destructive inspection and test. Tolerance study has been performed to avoid the mismatch of each fabricated component and to obtain the suitable tolerances in the assembly at the shop and site. This study is based on the experience in the fabrication of
International Nuclear Information System (INIS)
Qi, Kuo; Li, Xiaomin; Sun, Muhua; Huang, Qianming; Wei, Jiake; Xu, Zhi; Wang, Wenlong; Bai, Xuedong; Wang, Enge
2016-01-01
The phase transition from monoclinic WO 3 to cubic Li x WO 3 during lithiation of WO 3 is one of the key features for tungsten oxide as the most used electrochromic material. Conventionally, the lithium intercalation of WO 3 has been studied by building generic layered electrochromic device combining with structural characterization and electrochemistry measurement at macro scale. In-situ transmission electron microscopy (in-situ TEM) has been proposed as a method for revealing the detailed mechanism of structural, physical, and chemical properties. Here, we use in-situ TEM method to investigate the formation and evolution of Li x WO 3 in real-time during the electrochemical lithiation of WO 3 nanowires. The dynamic lithiation process is recorded by TEM imaging, diffraction, and electron energy loss spectroscopy. The WO 3 -Li x WO 3 phase boundary of reaction front has been observed at high resolution. The timeliness of crystallinity of Li x WO 3 and the intercalation channels for Li ions are also identified. Moreover, the co-existence of both polycrystalline Li-poor area and amorphous Li-rich phases of Li x WO 3 was found. Our results provide an insight into the basic lithiation process of WO 3 , which is significantly important for understanding the electrochromic mechanism of tungsten oxide.
International Nuclear Information System (INIS)
Ding Dexin; Yang Shijiao; Li Ming
1998-11-01
Bottom working is a very important structure for in-situ leaching of fragmented uranium ore by blasting. Its design and construction should simultaneously satisfy the requirements for receiving fragmented ore, transporting the ore, providing relief space for blast operation, passage for workers and fresh air for the slope and collecting the pregnant solution from spraying over the fragmented ore. The author deals with the design and construction of the complete water cutoff bottom working for collecting the pregnant solution for in-situ leaching of fragmented uranium ore by long hole blast in No. 745 mine in Guangdong Province. The preparation system for the block, the undercutting, the construction process and method of the bottom working and the measures to guide the solution leaked into the surrounding rock mass to the bottom of the block are described in detail
Sperm cryopreservation in endangered felids: developing linkage of in situ-ex situ populations.
Swanson, W F; Magarey, G M; Herrick, J R
2007-01-01
Many of the world's cat species face growing threats to their continued survival in nature. For some species, managed captive populations may provide a reservoir for future reintroduction or genetic augmentation. Because most zoo populations are derived from small founder sizes and are subject to loss of genetic variation over time, periodic infusion of founder alleles is necessary to avoid the dire consequences of inbreeding. Collection and freezing of semen from free-living nondomestic felids offers a viable option for introducing founder genes into captive populations without removal of animals from the wild. The effective application of this strategy requires established protocols for safely capturing and anaesthetising wild cats coupled with suitable methods of semen recovery, processing and cryopreservation under field conditions. In small-sized non-domestic felids, the general feasibility of this approach is being explored in two studies of black-footed cats and Pallas' cats. Two factors - relatively low sperm numbers per ejaculate and compromised status of frozen-thawed cat spermatozoa - suggest that in vitro fertilisation (IVF) and embryo transfer present the most efficient use of this limiting resource in small-sized cats. Our studies with captive felids have explored alternative methods of sperm cryopreservation that are adaptable to field situations and shown that frozen-thawed spermatozoa from Pallas' cats, ocelots, and fishing cats exhibit adequate function to fertilise heterologous and/or homologous oocytes in vitro. Most recently, we investigated the fertilising capacity of frozen-thawed spermatozoa obtained from wild Pallas' cats in Mongolia. Combined with improved methods for embryo culture and transfer in small cat species, sperm banking in situ will facilitate introduction of new founders into captive populations without causing further depletion of their wild counterparts. As one component of holistic conservation programs, including ongoing
In situ high-pressure measurement of crystal solubility by using neutron diffraction
Chen, Ji; Hu, Qiwei; Fang, Leiming; He, Duanwei; Chen, Xiping; Xie, Lei; Chen, Bo; Li, Xin; Ni, Xiaolin; Fan, Cong; Liang, Akun
2018-05-01
Crystal solubility is one of the most important thermo-physical properties and plays a key role in industrial applications, fundamental science, and geoscientific research. However, high-pressure in situ measurements of crystal solubility remain very challenging. Here, we present a method involving high-pressure neutron diffraction for making high-precision in situ measurements of crystal solubility as a function of pressure over a wide range of pressures. For these experiments, we designed a piston-cylinder cell with a large chamber volume for high-pressure neutron diffraction. The solution pressures are continuously monitored in situ based on the equation of state of the sample crystal. The solubility at a high pressure can be obtained by applying a Rietveld quantitative multiphase analysis. To evaluate the proposed method, we measured the high-pressure solubility of NaCl in water up to 610 MPa. At a low pressure, the results are consistent with the previous results measured ex situ. At a higher pressure, more reliable data could be provided by using an in situ high-pressure neutron diffraction method.
In situ macromolecular crystallography using microbeams.
Axford, Danny; Owen, Robin L; Aishima, Jun; Foadi, James; Morgan, Ann W; Robinson, James I; Nettleship, Joanne E; Owens, Raymond J; Moraes, Isabel; Fry, Elizabeth E; Grimes, Jonathan M; Harlos, Karl; Kotecha, Abhay; Ren, Jingshan; Sutton, Geoff; Walter, Thomas S; Stuart, David I; Evans, Gwyndaf
2012-05-01
Despite significant progress in high-throughput methods in macromolecular crystallography, the production of diffraction-quality crystals remains a major bottleneck. By recording diffraction in situ from crystals in their crystallization plates at room temperature, a number of problems associated with crystal handling and cryoprotection can be side-stepped. Using a dedicated goniometer installed on the microfocus macromolecular crystallography beamline I24 at Diamond Light Source, crystals have been studied in situ with an intense and flexible microfocus beam, allowing weakly diffracting samples to be assessed without a manual crystal-handling step but with good signal to noise, despite the background scatter from the plate. A number of case studies are reported: the structure solution of bovine enterovirus 2, crystallization screening of membrane proteins and complexes, and structure solution from crystallization hits produced via a high-throughput pipeline. These demonstrate the potential for in situ data collection and structure solution with microbeams. © 2012 International Union of Crystallography
International Nuclear Information System (INIS)
Yang Gongjun; Xu Jingjuan; Chen Hongyuan
2004-01-01
Based on the comprehensive analyses of the experimental results of the electrochemical methods, in situ UV-Vis absorption spectra, in situ electron spin resonance (ESR), and attenuated total-internal reflection (ATR) as well as the calculation of UV-Vis absorption data by PM3 Semi-Empirical method, a reaction mechanism for the redox processes of dobutamine was presented. When the anodic sweep is carried out, dobutamine firstly undergoes a free radical reaction with one-electron and one-proton to form semi-quinone free radicals, which will continuously convert to its corresponding quinone form by further electrochemical oxidation reaction. The formed quinone cannot only undergo a cyclization process by chemical reaction to produce a new compound, which can be reduced at more negative potential, but also be reduced to form dobutamine again when subsequent cathodic sweep is followed. The cyclization rate is depended upon pH values, and it increases with the increase of pH. In neutral medium, the corresponding oxidation form of the cyclization reaction product is easy to convert to melanin
International Nuclear Information System (INIS)
Al-Masri, M. S.; Doubal, A. W.
2010-12-01
Two nuclear analytical techniques (In-Situ Gamma ray spectrometry and laboratory gamma ray spectrometry) for determination of natural and artificial radionuclides in soil have been validated. The first technique depends on determination of radioactivity content of representative samples of the studied soil after laboratory preparation, while the second technique is based on direct determination of radioactivity content of soil using in-situ gamma-ray spectrometer. Analytical validation parameter such as detection limits, repeatability, reproducibility in addition to measurement uncertainties were estimated and compared for both techniques. Comparison results have shown that the determination of radioactivity in soil should apply the two techniques together where each of techniques is characterized by its low detection limit and uncertainty suitable for defined application of measurement. Radioactive isotopes in various locations were determined using the two methods by measuring 40 k, 238 U,and 137 Cs. The results showed that there are differences in attenuation factors due to soil moisture content differences; wet weight corrections should be applied when the two techniques are compared. (author)
Analysis of in situ stress at Yucca Mountain
International Nuclear Information System (INIS)
Bauer, S.J.; Holland, J.F.
1987-01-01
A method has been developed to initialize far-field finite element models such that the measured in situ stress state appears to be reproduced well. The method includes use of the mechanical stratigraphy, mechanical effect of pore pressure, gravity loading, a horizontal ''tectonic'' component of stress, and use of a jointed rock model to calculate the mechanical response. Topographic effects and effects related to the vertical variation in mechanical properties are predicted for repository depths (∼ 300 m). Gravity loading with a small horizontal compression is used to calculate a minimum horizontal stress similar in magnitude to that measured in situ. 8 refs., 5 figs
International Nuclear Information System (INIS)
Zombori, Peter
1995-01-01
The main aim of the present CRP is to identify the existing analytical methods and develop new ones, if possible, which provide rapid, reliable, and detailed information on the radioactive contamination of the environment after a major nuclear accident. Gamma spectrometry has long been regarded as one of the most applicable radioanalytical techniques but its use for environmental studies requires some further considerations. There are two possible approaches to measure environmental radioactivity: (a) taking samples of the different environmental media and measuring them in a laboratory or (b) taking the spectrometer to the place of interest and making in situ measurements. In the former case sampling is a crucial factor hindering the rapid analysis while the latter case is not always reliable due to the problems and uncertainties of the measurement interpretation. The application of in situ gamma spectrometry for the determination of environmental radioactivity has become increasingly attractive since the advent of the high resolution semiconductor gamma detectors, especially, more recently, portable high purity Ge diodes (HpGe). The applicability of this technique was very well proved after the Chernobyl reactor accident when in situ spectrometry played an important role in the rapid evaluation of the fall-out situation. Our measurements provided information on the amount and composition of the radioactive contamination of the ground surface already in the first hours. These measurements enabled us to predict the time variation of the environmental radioactivity after the stabilization of the situation. The portability of the system was an important factor in performing a rapid and efficient survey in different parts of the country. A serious disadvantage of this method is, however, that it requires some knowledge about the radionuclide distribution in the soil, which is normally determined by tedious and time consuming sample analysis of the different soil
Rock mechanics methods and in situ heater tests for design of a nuclear waste repository in basalt
International Nuclear Information System (INIS)
Board, M.P.
1978-01-01
Methods of integrating data from the Near-Surface Test Facility into the overall Waste Isolation Program are examined. Discussions are presented dealing primarily with the application of numerical models to the design of a waste repository. The various types of models currently available are discussed with reference to design in basalt and the breakdown of the problem of repository design is summarized. It is shown that the most efficient method for analyzing repository design is to break the problem down into several problems which are based on physical scale. These include the area directly surrounding a single waste canister (the very near field), the area including many canisters and canister emplacement rooms (the near field), and the area including the entire repository and the rock mass to the free surface (the far field). The methods by which numerical models are used for design are discussed. Flow charts are used to show the basic input data required, the calculational processes used, and the preliminary criteria for judgment of suitable repository performance. It is shown that the ultimate design of the allowable gross thermal loading density, and, thus, the layout of the underground workings is highly dependent upon the rock mass properties supplied as base line input data to the numerical models. Of the many input properties required, the thermal conductivity, the thermal expansion coefficient, and elastic moduli of the rock mass have, perhaps, the greatest effect on the calculation of induced temperatures, stresses, and displacements and, thus, repository design. To ensure that the design continues with confidence, field (in situ) values of input data must be obtained. The role of the Near-Surface Test Facility in situ testing in obtaining these basic required data is discussed
In situ shape and distance measurements in neutron scattering and diffraction
International Nuclear Information System (INIS)
Fujiwara, Satoru; Mendelson, R.A.
1994-01-01
Neutron scattering combined with selective isotopic labeling and contrast matching is useful for obtaining in situ structural information about a selected particle, or particles, in a macromolecular complex. The observed intensities, however, may be distorted by inter-complex interference and by scattering-length-density fluctuations of the (otherwise) contrast-matched portions. Methods have been proposed to cancel out such distortions (Hoppe's method, the Statistical Labeling Method, and the Triple Isotopic Substitution Method). With these methods as well as related unmixed-sample methods, structural information about the selected particles can be obtained without these distortions. We have generalized these methods so that, in addition to globular particles in solution, they can be applied to in situ structures of systems having underlying symmetry and/or net orientation as well. The information obtainable from such experiments is discussed
The application of geophysical logging at in-situ leaching uranium mine in China
International Nuclear Information System (INIS)
Liu Zeyao; Xu Shusheng; Li Zhongqiu
1999-01-01
The status of work, instrument and method employed for geophysical logging in different stages at in-situ leaching uranium mine are discussed and the development of software, electrical current logging and gamma ray logging are presented based on the requirement of in-situ leaching of uranium. In addition, new function and method with regard to home instrument are proposed for future work
Fan, Chen; Li, Nai; Cao, Xueli
2015-05-01
In-situ ionic liquid-dispersive liquid-liquid microextraction (IL-DLLME) method was developed as a pretreatment method for the detection of six chlorophenols (CPs) in honey samples. The hydrophobic ionic liquid [C4MIM][NTf2], formed in-situ by the hydrophilic ionic liquid [C4MIM][BF4] and the ion exchange reagent LiNTf2 was used as the microextractant solvent of CPs from honey sample. Then the enriched analytes were back-extracted into 40 μL of 0.14 M NaOH solution and finally subjected to analysis by high-performance liquid chromatography. The method showed low limit of detection of CPs, 0.8-3.2 μg/L and high enrichment factor, 34-65 with the recoveries range from 91.60% to 114.33%. The method is simple, rapid, environmentally friendly and with high extraction efficiency. Copyright © 2014 The Authors. Published by Elsevier Ltd.. All rights reserved.
International Nuclear Information System (INIS)
De Fraja Frangipane, E.; Andreottola, G.; Tatano, F.
1995-01-01
The present paper is an up-to-date overview of contaminated soil bioremediation techniques, which are analyzed in detail with regard to main process, engineering and design aspects. General biochemical/kinetic aspects of bioremediation of contaminated soil, and in situ treatments, are discussed in this part one
Atomic scissors: a new method of tracking the 5-bromo-2'-deoxyuridine-labeled DNA in situ.
Directory of Open Access Journals (Sweden)
Anna Ligasová
Full Text Available A new method of the light microscopy detection of BrdU-labeled DNA in situ is described. It is based on the oxidative attack at the deoxyribose moiety by copper(I in the presence of oxygen, which leads to the abstraction of hydrogen atom from deoxyribose culminating in the elimination of the nucleobase, scission of the nucleic-acid strand and formation of frequent gaps. The gaps allow the reaction of the antibodies with the commonly used markers of replication (e.g. 5-bromo-2'-deoxyuridine, which are otherwise masked. The method developed makes it possible to detect nuclear and mitochondrial DNA replication efficiently. In most cases, it does not inhibit effective protein detections and in addition enables simultaneous localization of newly-synthesized RNA. The alternative presently-used methods result in protein denaturation and/or extensive DNA cleavage followed by the DNA-bound proteins peeling off.
Key Features of Academic Detailing: Development of an Expert Consensus Using the Delphi Method.
Yeh, James S; Van Hoof, Thomas J; Fischer, Michael A
2016-02-01
Academic detailing is an outreach education technique that combines the direct social marketing traditionally used by pharmaceutical representatives with unbiased content summarizing the best evidence for a given clinical issue. Academic detailing is conducted with clinicians to encourage evidence-based practice in order to improve the quality of care and patient outcomes. The adoption of academic detailing has increased substantially since the original studies in the 1980s. However, the lack of standard agreement on its implementation makes the evaluation of academic detailing outcomes challenging. To identify consensus on the key elements of academic detailing among a group of experts with varying experiences in academic detailing. This study is based on an online survey of 20 experts with experience in academic detailing. We used the Delphi process, an iterative and systematic method of developing consensus within a group. We conducted 3 rounds of online surveys, which addressed 72 individual items derived from a previous literature review of 5 features of academic detailing, including (1) content, (2) communication process, (3) clinicians targeted, (4) change agents delivering intervention, and (5) context for intervention. Nonrespondents were removed from later rounds of the surveys. For most questions, a 4-point ordinal scale was used for responses. We defined consensus agreement as 70% of respondents for a single rating category or 80% for dichotomized ratings. The overall survey response rate was 95% (54 of 57 surveys) and nearly 92% consensus agreement on the survey items (66 of 72 items) by the end of the Delphi exercise. The experts' responses suggested that (1) focused clinician education offering support for clinical decision-making is a key component of academic detailing, (2) detailing messages need to be tailored and provide feasible strategies and solutions to challenging cases, and (3) academic detailers need to develop specific skill sets
In situ detrital zircon (U-Th)/He thermochronology
Tripathy, A.; Monteleone, B. D.; van Soest, M. C.; Hodges, K.; Hourigan, J. K.
2010-12-01
Detrital studies of both sand and rock are relevant to many problems, ranging from the climate and tectonics feedback debate to the long-term record of orogenic evolution. When applying the conventional (U-Th)/He technique to such studies, two important issues arise. Often, only euhedral grains are permissible for analysis in order to make simple geometric corrections for α-recoil. In detrital samples, this is problematic because euhedral grains can be scarce due to mechanical abrasion during transport, and potentially introduce bias in favour of more proximally sourced grains. Second, inherent to detrital studies is the need to date many grains (>100) per sample to ensure a representative sampling of the sediment source region, thus making robust conventional detrital studies both expensive and time-consuming. UV laser microprobes can improve this by permitting careful targeting of the grain interior away from the α-ejection zone, rendering the α-recoil correction unnecessary, thus eliminating bias toward euhedral grains. In the Noble Gas, Geochemistry, and Geochronology Laboratory at ASU, apatite and zircon have been successfully dated using in situ methods. For this study, the conventional and in situ techniques are compared by dating zircons from a modern river sand that drains a small catchment in the Mesozoic-Cenozoic Ladakh Batholith in NW India. This sample has a simple provenance, which allows us to demonstrate the robustness of the in situ method. Moreover, different microbeam techniques will be explored to establish the most efficient approach to obtain accurate and precise U-Th concentrations using synrock, which is our powdered, homogenized, and reconstituted zircon-rock standard. Without this, such in situ U-Th data would be difficult to obtain. 117 zircons were dated using the conventional (U-Th)/He method, revealing dates ranging from 9.70±0.35 to 106.6±3.5 Ma (2σ) with the major mode at 26 Ma. For comparison, 44 grains were dated using the in
International Nuclear Information System (INIS)
Hartung, W.H.; Asner, D.M.; Conway, J.V.; Dennett, C.A.; Greenwald, S.; Kim, J.-S.; Li, Y.; Moore, T.P.; Omanovic, V.; Palmer, M.A.; Strohman, C.R.
2015-01-01
The performance of a particle accelerator can be limited by the build-up of an electron cloud (EC) in the vacuum chamber. Secondary electron emission from the chamber walls can contribute to EC growth. An apparatus for in-situ measurements of the secondary electron yield (SEY) in the Cornell Electron Storage Ring (CESR) was developed in connection with EC studies for the CESR Test Accelerator program. The CESR in-situ system, in operation since 2010, allows for SEY measurements as a function of incident electron energy and angle on samples that are exposed to the accelerator environment, typically 5.3 GeV counter-rotating beams of electrons and positrons. The system was designed for periodic measurements to observe beam conditioning of the SEY with discrimination between exposure to direct photons from synchrotron radiation versus scattered photons and cloud electrons. The samples can be exchanged without venting the CESR vacuum chamber. Measurements have been done on metal surfaces and EC-mitigation coatings. The in-situ SEY apparatus and improvements to the measurement tools and techniques are described
Energy Technology Data Exchange (ETDEWEB)
Hartung, W.H., E-mail: wh29@cornell.edu; Asner, D.M.; Conway, J.V.; Dennett, C.A.; Greenwald, S.; Kim, J.-S.; Li, Y.; Moore, T.P.; Omanovic, V.; Palmer, M.A.; Strohman, C.R.
2015-05-21
The performance of a particle accelerator can be limited by the build-up of an electron cloud (EC) in the vacuum chamber. Secondary electron emission from the chamber walls can contribute to EC growth. An apparatus for in-situ measurements of the secondary electron yield (SEY) in the Cornell Electron Storage Ring (CESR) was developed in connection with EC studies for the CESR Test Accelerator program. The CESR in-situ system, in operation since 2010, allows for SEY measurements as a function of incident electron energy and angle on samples that are exposed to the accelerator environment, typically 5.3 GeV counter-rotating beams of electrons and positrons. The system was designed for periodic measurements to observe beam conditioning of the SEY with discrimination between exposure to direct photons from synchrotron radiation versus scattered photons and cloud electrons. The samples can be exchanged without venting the CESR vacuum chamber. Measurements have been done on metal surfaces and EC-mitigation coatings. The in-situ SEY apparatus and improvements to the measurement tools and techniques are described.
Alshakova, E. L.
2017-01-01
The program in the AutoLISP language allows automatically to form parametrical drawings during the work in the AutoCAD software product. Students study development of programs on AutoLISP language with the use of the methodical complex containing methodical instructions in which real examples of creation of images and drawings are realized. Methodical instructions contain reference information necessary for the performance of the offered tasks. The method of step-by-step development of the program is the basis for training in programming on AutoLISP language: the program draws elements of the drawing of a detail by means of definitely created function which values of arguments register in that sequence in which AutoCAD gives out inquiries when performing the corresponding command in the editor. The process of the program design is reduced to the process of step-by-step formation of functions and sequence of their calls. The author considers the development of the AutoLISP program for the creation of parametrical drawings of details, the defined design, the user enters the dimensions of elements of details. These programs generate variants of tasks of the graphic works performed in educational process of "Engineering graphics", "Engineering and computer graphics" disciplines. Individual tasks allow to develop at students skills of independent work in reading and creation of drawings, as well as 3D modeling.
Gas injection to inhibit migration during an in situ heat treatment process
Kuhlman, Myron Ira; Vinegar; Harold J.; Baker, Ralph Sterman; Heron, Goren
2010-11-30
Methods of treating a subsurface formation are described herein. Methods for treating a subsurface treatment area in a formation may include introducing a fluid into the formation from a plurality of wells offset from a treatment area of an in situ heat treatment process to inhibit outward migration of formation fluid from the in situ heat treatment process.
International Nuclear Information System (INIS)
McGinnis, M. J.; Pessiki, S.
2006-01-01
The core-drilling method is an emerging technique for evaluating in-situ stress in a concrete structure. A small hole is drilled into the structure, and the deformations in the vicinity of the hole are measured and related via elasticity theory to the stress. The method is similar to the ASTM hole-drilling strain-gauge method excepting that displacements rather than strains are the measured quantities. The technique may be considered nondestructive since the ability of the structure to perform its function is unaffected, and the hole is easily repaired. Displacement measurements in the current work are performed using 3D digital image correlation and industrial photogrammetry. The current paper addresses perturbations in the method caused by steel reinforcement within the concrete. The reinforcement is significantly stiffer than the surrounding concrete, altering the expected displacement field. A numerical investigation performed indicates an under-prediction of stress by as much as 18 percent in a heavily reinforced structure, although the effect is significantly smaller for more common amounts of reinforcement
McGinnis, M. J.; Pessiki, S.
2006-03-01
The core-drilling method is an emerging technique for evaluating in-situ stress in a concrete structure. A small hole is drilled into the structure, and the deformations in the vicinity of the hole are measured and related via elasticity theory to the stress. The method is similar to the ASTM hole-drilling strain-gauge method excepting that displacements rather than strains are the measured quantities. The technique may be considered nondestructive since the ability of the structure to perform its function is unaffected, and the hole is easily repaired. Displacement measurements in the current work are performed using 3D digital image correlation and industrial photogrammetry. The current paper addresses perturbations in the method caused by steel reinforcement within the concrete. The reinforcement is significantly stiffer than the surrounding concrete, altering the expected displacement field. A numerical investigation performed indicates an under-prediction of stress by as much as 18 percent in a heavily reinforced structure, although the effect is significantly smaller for more common amounts of reinforcement.
Energy Technology Data Exchange (ETDEWEB)
Burke, M.G., E-mail: m.g.burke@manchester.ac.uk; Bertali, G.; Prestat, E.; Scenini, F.; Haigh, S.J.
2017-05-15
In situ analytical transmission electron microscopy (TEM) can provide a unique perspective on dynamic reactions in a variety of environments, including liquids and gases. In this study, in situ analytical TEM techniques have been applied to examine the localised oxidation reactions that occur in a Ni-Cr-Fe alloy, Alloy 600, using a gas environmental cell at elevated temperatures. The initial stages of preferential intergranular oxidation, shown to be an important precursor phenomenon for intergranular stress corrosion cracking in pressurized water reactors (PWRs), have been successfully identified using the in situ approach. Furthermore, the detailed observations correspond to the ex situ results obtained from bulk specimens tested in hydrogenated steam and in high temperature PWR primary water. The excellent agreement between the in situ and ex situ oxidation studies demonstrates that this approach can be used to investigate the initial stages of preferential intergranular oxidation relevant to nuclear power systems. - Highlights: • In situ analytical TEM has been performed in 1 bar H{sub 2}-H{sub 2}O vapor at 360–480 °C. • Nanoscale GB migration and solute partitioning correlate with ex situ data for Alloy 600 in H{sub 2}-steam. • This technique can provide new insights into localised reactions associated with localised oxidation.
In situ shape and distance measurements in neutron scattering and diffraction
Energy Technology Data Exchange (ETDEWEB)
Fujiwara, Satoru; Mendelson, R.A. [Univ. of California, San Francisco, CA (United States)
1994-12-31
Neutron scattering combined with selective isotopic labeling and contrast matching is useful for obtaining in situ structural information about a selected particle, or particles, in a macromolecular complex. The observed intensities, however, may be distorted by inter-complex interference and by scattering-length-density fluctuations of the (otherwise) contrast-matched portions. Methods have been proposed to cancel out such distortions (Hoppe`s method, the Statistical Labeling Method, and the Triple Isotopic Substitution Method). With these methods as well as related unmixed-sample methods, structural information about the selected particles can be obtained without these distortions. We have generalized these methods so that, in addition to globular particles in solution, they can be applied to in situ structures of systems having underlying symmetry and/or net orientation as well. The information obtainable from such experiments is discussed.
Rezaei, R.; Dinkelacker, F.; Tilch, B.; Delebinski, T.; Brauer, M.
2016-01-01
Enhancing the predictive quality of engine models, while maintaining an affordable computational cost, is of great importance. In this study, a phenomenological combustion and a tabulated NOx model, focusing on efficient modeling and improvement of computational effort, is presented. The proposed approach employs physical and chemical sub-models for local processes such as injection, spray formation, ignition, combustion, and NOx formation, being based on detailed tabulated chemistry methods....
Detail analysis of fusion neutronics benchmark experiment on beryllium
International Nuclear Information System (INIS)
Konno, Chikara; Ochiai, Kentaro; Takakura, Kosuke; Ohnishi, Seiki; Kondo, Keitaro; Wada, Masayuki; Sato, Satoshi
2010-01-01
Our previous analysis of the integral experiments (in situ and TOF experiments) on beryllium with DT neutrons at JAEA/FNS pointed out two problems by using MCNP4C and the latest nuclear data libraries; one was a strange larger neutron peak around 12 MeV appearing in the TOF experiment analysis with JEFF-3.1 and the other was an overestimation on law energy neutrons in the in situ experiment analyses with all the nuclear data libraries. We investigated reasons for these problems in detail. It was found out that the official ACE file MCJEFF3.1 of JEFF-3.1 had an inconsistency with the original JEFF-3.1, which caused the strange larger neutron peak around 12 MeV in the TOF experiment analysis. We also found out that the calculated thermal neutron peak was probably too large in the in situ experiment. On trial we examined influence of the thermal neutron scattering law data of beryllium metal in ENDF/B-VI. The result pointed out that the coherent elastic scattering cross-section data in the thermal neutron scattering law data of beryllium metal were probably too large.
International Nuclear Information System (INIS)
Sittner, P.; Novak, V.; Landa, M.; Lukas, P.
2007-01-01
The unique thermomechanical functions of shape memory alloys (hysteretic stress-strain-temperature responses) not their structural properties (as strength, fatigue, corrosion resistance, etc.) are primarily utilized in engineering applications. In order to better understand and predict the functional behavior, we have recently employed two dedicated non-invasive in situ experimental methods capable to follow the deformation/transformation processes in thermomechanically loaded polycrystalline samples. The in situ neutron diffraction method takes advantage of the ability of thermal neutrons to penetrate bulk samples. As a diffraction technique sensitive to interplanar spacings in crystalline solids, it provides in situ information on the changes in crystal structure, phase composition, phase stress and texture in the transforming samples. The combined in situ ultrasonic and electric resistance method follows variations of the electric resistance as well as speed and attenuation of acoustic waves propagating through the transforming sample. The acoustic waves are mainly sensitive to changes of elastic properties accompanying the deformation/transformation processes. The latter method thus follows the changes in interatomic bonds rather than changes in the interplanar lattice spacings focused in the neutron diffraction method. The methods are thus complementary. They are briefly described and selected experimental results obtained recently on NiTi alloys are presented and discussed
In situ macromolecular crystallography using microbeams
International Nuclear Information System (INIS)
Axford, Danny; Owen, Robin L.; Aishima, Jun; Foadi, James; Morgan, Ann W.; Robinson, James I.; Nettleship, Joanne E.; Owens, Raymond J.; Moraes, Isabel; Fry, Elizabeth E.; Grimes, Jonathan M.; Harlos, Karl; Kotecha, Abhay; Ren, Jingshan; Sutton, Geoff; Walter, Thomas S.; Stuart, David I.; Evans, Gwyndaf
2012-01-01
A sample environment for mounting crystallization trays has been developed on the microfocus beamline I24 at Diamond Light Source. The technical developments and several case studies are described. Despite significant progress in high-throughput methods in macromolecular crystallography, the production of diffraction-quality crystals remains a major bottleneck. By recording diffraction in situ from crystals in their crystallization plates at room temperature, a number of problems associated with crystal handling and cryoprotection can be side-stepped. Using a dedicated goniometer installed on the microfocus macromolecular crystallography beamline I24 at Diamond Light Source, crystals have been studied in situ with an intense and flexible microfocus beam, allowing weakly diffracting samples to be assessed without a manual crystal-handling step but with good signal to noise, despite the background scatter from the plate. A number of case studies are reported: the structure solution of bovine enterovirus 2, crystallization screening of membrane proteins and complexes, and structure solution from crystallization hits produced via a high-throughput pipeline. These demonstrate the potential for in situ data collection and structure solution with microbeams
In situ macromolecular crystallography using microbeams
Energy Technology Data Exchange (ETDEWEB)
Axford, Danny; Owen, Robin L.; Aishima, Jun [Diamond Light Source, Harwell Science and Innovation Campus, Didcot, Oxfordshire OX11 0DE (United Kingdom); Foadi, James [Diamond Light Source, Harwell Science and Innovation Campus, Didcot, Oxfordshire OX11 0DE (United Kingdom); Imperial College, London SW7 2AZ (United Kingdom); Morgan, Ann W.; Robinson, James I. [University of Leeds, Leeds LS9 7FT (United Kingdom); Nettleship, Joanne E.; Owens, Raymond J. [Research Complex at Harwell, Rutherford Appleton Laboratory R92, Didcot, Oxfordshire OX11 0DE (United Kingdom); Moraes, Isabel [Imperial College, London SW7 2AZ (United Kingdom); Fry, Elizabeth E.; Grimes, Jonathan M.; Harlos, Karl; Kotecha, Abhay; Ren, Jingshan; Sutton, Geoff; Walter, Thomas S. [University of Oxford, Roosevelt Drive, Oxford OX3 7BN (United Kingdom); Stuart, David I. [Diamond Light Source, Harwell Science and Innovation Campus, Didcot, Oxfordshire OX11 0DE (United Kingdom); University of Oxford, Roosevelt Drive, Oxford OX3 7BN (United Kingdom); Evans, Gwyndaf, E-mail: gwyndaf.evans@diamond.ac.uk [Diamond Light Source, Harwell Science and Innovation Campus, Didcot, Oxfordshire OX11 0DE (United Kingdom)
2012-04-17
A sample environment for mounting crystallization trays has been developed on the microfocus beamline I24 at Diamond Light Source. The technical developments and several case studies are described. Despite significant progress in high-throughput methods in macromolecular crystallography, the production of diffraction-quality crystals remains a major bottleneck. By recording diffraction in situ from crystals in their crystallization plates at room temperature, a number of problems associated with crystal handling and cryoprotection can be side-stepped. Using a dedicated goniometer installed on the microfocus macromolecular crystallography beamline I24 at Diamond Light Source, crystals have been studied in situ with an intense and flexible microfocus beam, allowing weakly diffracting samples to be assessed without a manual crystal-handling step but with good signal to noise, despite the background scatter from the plate. A number of case studies are reported: the structure solution of bovine enterovirus 2, crystallization screening of membrane proteins and complexes, and structure solution from crystallization hits produced via a high-throughput pipeline. These demonstrate the potential for in situ data collection and structure solution with microbeams.
Precise and in situ genetic humanization of 6 Mb of mouse immunoglobulin genes.
Macdonald, Lynn E; Karow, Margaret; Stevens, Sean; Auerbach, Wojtek; Poueymirou, William T; Yasenchak, Jason; Frendewey, David; Valenzuela, David M; Giallourakis, Cosmas C; Alt, Frederick W; Yancopoulos, George D; Murphy, Andrew J
2014-04-08
Genetic humanization, which involves replacing mouse genes with their human counterparts, can create powerful animal models for the study of human genes and diseases. One important example of genetic humanization involves mice humanized for their Ig genes, allowing for human antibody responses within a mouse background (HumAb mice) and also providing a valuable platform for the generation of fully human antibodies as therapeutics. However, existing HumAb mice do not have fully functional immune systems, perhaps because of the manner in which they were genetically humanized. Heretofore, most genetic humanizations have involved disruption of the endogenous mouse gene with simultaneous introduction of a human transgene at a new and random location (so-called KO-plus-transgenic humanization). More recent efforts have attempted to replace mouse genes with their human counterparts at the same genetic location (in situ humanization), but such efforts involved laborious procedures and were limited in size and precision. We describe a general and efficient method for very large, in situ, and precise genetic humanization using large compound bacterial artificial chromosome-based targeting vectors introduced into mouse ES cells. We applied this method to genetically humanize 3-Mb segments of both the mouse heavy and κ light chain Ig loci, by far the largest genetic humanizations ever described. This paper provides a detailed description of our genetic humanization approach, and the companion paper reports that the humoral immune systems of mice bearing these genetically humanized loci function as efficiently as those of WT mice.
The theory and method of two-well field test for in-situ leaching uranium
International Nuclear Information System (INIS)
Yao Yixuan; Huo Jiandang; Xiang Qiulin; Tang Baobin
2007-01-01
Because leaching area in field test for in-situ leaching uranium is not accounted exactly, the reliability of obtaining parameters by calculating can not be ensured, and the whole test needs a long time and great investment. In two-well field test, lixiviant is injected from one well, pregnant solution is pumped out from the other, flow rate of the production well is more than that of the injection well, and uranium is not recoveried. In the case of keeping invariable ratio of pumping capacity to injecting capacity during the testing process, leaching area is not variable, can be exactly calculated. The full field test needs six months to one year. Two-well test is a scientific, rapid, minimal spending field test method, and is widely used in Commonwealth of Independent States. (authors)
Jiang, Chenchen; Lu, Haojian; Zhang, Hongti; Shen, Yajing; Lu, Yang
2017-01-01
In the past decades, in situ scanning electron microscopy (SEM) has become a powerful technique for the experimental study of low-dimensional (1D/2D) nanomaterials, since it can provide unprecedented details for individual nanostructures upon mechanical and electrical stimulus and thus uncover the fundamental deformation and failure mechanisms for their device applications. In this overview, we summarized recent developments on in situ SEM-based mechanical and electrical characterization techniques including tensile, compression, bending, and electrical property probing on individual nanostructures, as well as the state-of-the-art electromechanical coupling analysis. In addition, the advantages and disadvantages of in situ SEM tests were also discussed with some possible solutions to address the challenges. Furthermore, critical challenges were also discussed for the development and design of robust in situ SEM characterization platform with higher resolution and wider range of samples. These experimental efforts have offered in-depth understanding on the mechanical and electrical properties of low-dimensional nanomaterial components and given guidelines for their further structural and functional applications.
Directory of Open Access Journals (Sweden)
Chenchen Jiang
2017-01-01
Full Text Available In the past decades, in situ scanning electron microscopy (SEM has become a powerful technique for the experimental study of low-dimensional (1D/2D nanomaterials, since it can provide unprecedented details for individual nanostructures upon mechanical and electrical stimulus and thus uncover the fundamental deformation and failure mechanisms for their device applications. In this overview, we summarized recent developments on in situ SEM-based mechanical and electrical characterization techniques including tensile, compression, bending, and electrical property probing on individual nanostructures, as well as the state-of-the-art electromechanical coupling analysis. In addition, the advantages and disadvantages of in situ SEM tests were also discussed with some possible solutions to address the challenges. Furthermore, critical challenges were also discussed for the development and design of robust in situ SEM characterization platform with higher resolution and wider range of samples. These experimental efforts have offered in-depth understanding on the mechanical and electrical properties of low-dimensional nanomaterial components and given guidelines for their further structural and functional applications.
International Nuclear Information System (INIS)
Zombori, P.; Andrasi, A.; Nemeth, I.
1995-01-01
In case of major nuclear accidents when larger amount of radioactive material is released into the atmosphere vast areas can become contaminated by the nuclear fallout. The deposited radioactivity penetrates the soil in a complex manner: dry and wet deposition lead to different initial distribution patterns which are further modified by the later transport processes in the upper layers of the soil. The distribution is influenced by various factors (physico-chemical characteristics of the radioisotopes, soil type, weather conditions, environment etc.), the resulting soil profile is hardly predictable. An important lesson we learned from the Chernobyl reactor accident is the great variability of the contamination both in the extent of the deposition and in the penetration features. In recent years - following the reactor accident in Chernobyl - an increased interest for rapid methods of monitoring environmental radioactivity was expressed. The International Atomic Energy Agency initiated a research project to co-ordinate the activities carried out in various laboratories aiming at the development of rapid monitoring procedures. The Co-ordinated Research Project (CRP) G6 10 01 under the title Rapid Instrumental and Separation Methods for Monitoring Radionuclides in Food and the Environment has given a frame for 11 research programs. The Health Physics Department of the KFKI Institute for Atomic Energy Research (the former Central Research Institute for Physics) has taken a part in this CRP with a project titled: Rapid In Situ Gamma Spectrometric Determination of Fallout Radioactivity in the Environment. The main objective of our study was to find a method to estimate the penetration characteristics of the fallout radioactivity by using only spectral information obtained by the in situ spectrometric measurement thus avoiding the need for a long and tiresome sampling and sample analysis procedure
Printing Fabrication of Bulk Heterojunction Solar Cells and In Situ Morphology Characterization.
Liu, Feng; Ferdous, Sunzida; Wan, Xianjian; Zhu, Chenhui; Schaible, Eric; Hexemer, Alexander; Wang, Cheng; Russell, Thomas P
2017-01-29
Polymer-based materials hold promise as low-cost, flexible efficient photovoltaic devices. Most laboratory efforts to achieve high performance devices have used devices prepared by spin coating, a process that is not amenable to large-scale fabrication. This mismatch in device fabrication makes it difficult to translate quantitative results obtained in the laboratory to the commercial level, making optimization difficult. Using a mini-slot die coater, this mismatch can be resolved by translating the commercial process to the laboratory and characterizing the structure formation in the active layer of the device in real time and in situ as films are coated onto a substrate. The evolution of the morphology was characterized under different conditions, allowing us to propose a mechanism by which the structures form and grow. This mini-slot die coater offers a simple, convenient, material efficient route by which the morphology in the active layer can be optimized under industrially relevant conditions. The goal of this protocol is to show experimental details of how a solar cell device is fabricated using a mini-slot die coater and technical details of running in situ structure characterization using the mini-slot die coater.
In situ hybridization; principles and applications: review article
Directory of Open Access Journals (Sweden)
Zahra Nozhat
2015-06-01
Full Text Available In situ hybridization (ISH is a method that uses labeled complementary single strand DNA or RNA to localize specific DNA or RNA sequences in an intact cell or in a fixed tissue section. The main steps of ISH consist of: probe selection, tissue or sample preparation, pre-hybridization treatment, hybridization and washing, detection and control procedure. Probe selection is one of the important aspects of successful hybridization. ISH sensitivity and specificity can be influenced by: probe construct, efficiency of labeling, percentage of GC, probe length and signal detection systems. Different methods such as nick translation, random priming, end tailing and T4 DNA polymerase replacement are used for probe generation. Both radioactive and non-radioactive labels can be used in order to probe labeling. Nucleic acid maintenance in samples, prevention of morphological changes of samples and probe penetration into tissue section are the main aims of sample preparation step. Then, a small amount of solution containing probe, is added on slides containing tissue sections for hybridization process, then slides are incubated overnight. Next day, washes are carried out to remove the probes which are not bound to target DNA or RNA. Finally, in order to be sure that the observed labeling is specific to the target sequence, using several control procedures is very important. Various techniques based on ISH consist of: Fluorescence in situ hybridization (FISH, chromogenic in situ hybridization (CISH, genomic in situ hybridization (GISH, comparative genomic hybridization (CGH, spectral karyotyping (SKY and multiplex fluorescence in situ hybridization (MFISH. One of the most common techniques of ISH is fluorescence in situ hybridization. FISH can be used to: 1 detect small deletions and duplications that are not visible using microscope analysis, 2 detect how many chromosomes of a certain type are present in each cell and 3 confirm rearrangements that are
Interpretation of the Haestholmen in situ state of stress based on core damage observations
International Nuclear Information System (INIS)
Hakala, M.
2000-01-01
At the Haestholmen investigation site, direct in situ stress measurements, overcoring and hydraulic fracturing have been unsuccessful because of ring disking and horizontal hydraulic fracturing. Prior to this study, a detailed study on both core disking and ring disking was made, and based on those results an in situ state of stress interpretation method was developed. In this work this method is applied to the Haestholmen site. The interpretation is based on disk fracture type, spacing and shape. Also, the Hoek-Brown strength envelope and Poisson's ratio of intact rock are needed. The interpretation result is most reliable if both core disking and ring disking information at the same depth levels is available. A detailed core logging showed that ring disking is systematic below the -365 m level in the vertical overcoring stress measurement hole, HH-KR6. On the other hand, no representative core disking exists except for two points in two differently oriented subvertical boreholes HH-KR2 and HHKR7. Because the interpretation has to be based on ring disking only, upper and lower estimates for the vertical stress were set. These were gravitational and 67% of gravitational. Furthermore, the in situ stress state was assumed to be in horizontal and vertical planes, because the disking in vertical borehole HH-KR6 was not inclined. The interpretation resulted in a good estimate for the major horizontal stress but none of the horizontal stress rations ( 0.25, 0.5, 0.75 and 1.0 ) or vertical stress assumptions studied are clearly more probable the others. At the 500 m level the resulting maximum horizontal stress is 41 MPa. If a linear fit through the zero depth and zero stress point is applied, the maximum horizontal stress gradient is 0.0818 z MPa/m with a standard deviation between 5 and 12 per cent. The orientation of the major horizontal stress is 108 with standard deviation of 21 degrees. The interpreted major horizontal stress state also indicated that systematic
In Situ Magnetic Separation for Extracellular Protein Production
DEFF Research Database (Denmark)
Kappler, T.; Cerff, Martin; Ottow, Kim Ekelund
2009-01-01
A new approach for in situ product removal from bioreactors is presented in which high-gradient magnetic separation is used. This separation process was used for the adsorptive removal of proteases secreted by Bacillus licheniformis. Small, non-porous bacitracin linked magnetic adsorbents were...... was not influenced by the in situ product removal step. Protease production also remained the same after the separation step. Furthermore, degradation of the protease, which followed first order kinetics, was reduced by using the method. Using a theoretical modeling approach, we Could show that protease yield...... in total was enhanced by using in situ magnetic separation. The process described here is a promising technique to improve overall yield in No production processes which are often limited due to weak downstream operations, Potential limitations encountered during a bioprocess can be overcome...
International Nuclear Information System (INIS)
Kolev, B.
2006-01-01
Four in situ methods for potential and actual evapotranspiration determining were compared: neutron gauge, tensiometers, gypsum blocks and lysimeters. The actual and potential evapotranspiration were calculated by water balance equation and by using a simulation model for their determination. The aim of this study was mainly pointed on calculations of water use efficiency and transpiration coefficient in potential production situation. This makes possible to choose the best way for water consumption optimization for a given crop. The final results find with the best of the methods could be used for applying the principles of sustainable agricultural production in random object of Bulgarian agricultural area
CSIR Research Space (South Africa)
Snedden, Glen C
2003-09-01
Full Text Available Institute of Aeronautics and Astronautics Inc. All rights reserved. DETAILED DISC ASSEMBLY TEMPERATURE PREDICTION: COMPARISON BETWEEN CFD AND SIMPLIFIED ENGINEERING METHODS ISABE-2005-1130 Glen Snedden, Thomas Roos and Kavendra Naidoo CSIR, Defencetek... transfer and conduction code (Gaugler, 1978) Taw Adiabatic Wall Temperature y+ Near wall Reynolds number Introduction In order to calculate life degradation of gas turbine disc assemblies, it is necessary to model the transient thermal and mechanical...
In-situ SEM microchip setup for electrochemical experiments with water based solutions
International Nuclear Information System (INIS)
Jensen, E.; Købler, C.; Jensen, P.S.; Mølhave, K.
2013-01-01
Studying electrochemical (EC) processes with electron microscopes offers the possibility of achieving much higher resolution imaging of nanoscale processes in real time than with optical microscopes. We have developed a vacuum sealed liquid sample electrochemical cell with electron transparent windows, microelectrodes and an electrochemical reference electrode. The system, called the EC-SEM Cell, is used to study electrochemical reactions in liquid with a standard scanning electron microscope (SEM). The central component is a microfabricated chip with a thin (50 nm) Si-rich silicon nitride (SiNx) window with lithographically defined platinum microelectrodes. We show here the design principles of the EC-SEM system, its detailed construction and how it has been used to perform a range of EC experiments, two of which are presented here. It is shown that the EC-SEM Cell can survive extended in-situ EC experiments. Before the EC experiments we characterized the beam current being deposited in the liquid as this will affect the experiments. The first EC experiment shows the influence of the electron-beam (e-beam) on a nickel solution by inducing electroless nickel deposition on the window when increasing the current density from the e-beam. The second experiment shows electrolysis in EC-SEM Cell, induced by the built-in electrodes. - Highlights: • New in-situ SEM system for electrochemistry. • In-situ Beam current measurements through liquid. • In-situ SEM E-beam-induced electroless deposition of Ni. • In-situ electrolysis
In-situ SEM microchip setup for electrochemical experiments with water based solutions
Energy Technology Data Exchange (ETDEWEB)
Jensen, E., E-mail: eric.jensen@nanotech.dtu.dk [DTU Nanotech, Technical University of Denmark, Ørsteds Plads, Building 345E, 2800 Kongens Lyngby (Denmark); DTU CEN, Technical University of Denmark, Fysikvej, Building 307, 2800 Kongens Lyngby (Denmark); Købler, C., E-mail: carsten.kobler@nanotech.dtu.dk [DTU Nanotech, Technical University of Denmark, Ørsteds Plads, Building 345E, 2800 Kongens Lyngby (Denmark); DTU CEN, Technical University of Denmark, Fysikvej, Building 307, 2800 Kongens Lyngby (Denmark); Jensen, P.S., E-mail: psj@kemi.dtu.dk [DTU Kemi, Technical University of Denmark, Kemitorvet, Building 207, 2800 Kongens Lyngby (Denmark); Mølhave, K., E-mail: kristian.molhave@nanotech.dtu.dk [DTU Nanotech, Technical University of Denmark, Ørsteds Plads, Building 345E, 2800 Kongens Lyngby (Denmark)
2013-06-15
Studying electrochemical (EC) processes with electron microscopes offers the possibility of achieving much higher resolution imaging of nanoscale processes in real time than with optical microscopes. We have developed a vacuum sealed liquid sample electrochemical cell with electron transparent windows, microelectrodes and an electrochemical reference electrode. The system, called the EC-SEM Cell, is used to study electrochemical reactions in liquid with a standard scanning electron microscope (SEM). The central component is a microfabricated chip with a thin (50 nm) Si-rich silicon nitride (SiNx) window with lithographically defined platinum microelectrodes. We show here the design principles of the EC-SEM system, its detailed construction and how it has been used to perform a range of EC experiments, two of which are presented here. It is shown that the EC-SEM Cell can survive extended in-situ EC experiments. Before the EC experiments we characterized the beam current being deposited in the liquid as this will affect the experiments. The first EC experiment shows the influence of the electron-beam (e-beam) on a nickel solution by inducing electroless nickel deposition on the window when increasing the current density from the e-beam. The second experiment shows electrolysis in EC-SEM Cell, induced by the built-in electrodes. - Highlights: • New in-situ SEM system for electrochemistry. • In-situ Beam current measurements through liquid. • In-situ SEM E-beam-induced electroless deposition of Ni. • In-situ electrolysis.
Martini, A.; Lomachenko, K. A.; Pankin, I. A.; Negri, C.; Berlier, G.; Beato, P.; Falsig, H.; Bordiga, S.; Lamberti, C.
2017-01-01
The small pore Cu-CHA zeolite is attracting increasing attention as a versatile platform to design novel single-site catalysts for deNOx applications and for the direct conversion of methane to methanol. Understanding at the atomic scale how the catalyst composition influences the Cu-species formed during thermal activation is a key step to unveil the relevant composition–activity relationships. Herein, we explore by in situ XAS the impact of Cu-CHA catalyst composition on temperature-dependent Cu-speciation and reducibility. Advanced multivariate analysis of in situ XANES in combination with DFT-assisted simulation of XANES spectra and multi-component EXAFS fits as well as in situ FTIR spectroscopy of adsorbed N2 allow us to obtain unprecedented quantitative structural information on the complex dynamics during the speciation of Cu-sites inside the framework of the CHA zeolite. PMID:29147509
Engineering feasibility analysis for in-situ stabilization of Canonsburg residues
International Nuclear Information System (INIS)
1982-01-01
The US Department of Energy is considering several methods for carrying out remedial actions in Canonsburg, Pennsylvania, at the site of an inactive uranium-processing mill. The main objective of this study is to determine the feasibility of in-situ stabilization as the remedial action. In-situ stabilization is an alternative to site decontamination and offsite disposal. The problems associated with offsite hauling of large quantities of contaminated material and with the location and development of a new disposal site could be avoided by the implementation of an in-situ stabilization concept. In addition, the in-situ approach would be more cost-effective than offsite disposal. This study will establish that a technically feasible and implementable in-situ stabilization concept can be developed that meets regulatory requirements and is cost effective. This study in no way commits the DOE to implement any specific actions described herein. 11 refs., 30 figs., 24 tabs
Automated gas bubble imaging at sea floor – a new method of in situ gas flux quantification
Directory of Open Access Journals (Sweden)
G. Bohrmann
2010-06-01
Full Text Available Photo-optical systems are common in marine sciences and have been extensively used in coastal and deep-sea research. However, due to technical limitations in the past photo images had to be processed manually or semi-automatically. Recent advances in technology have rapidly improved image recording, storage and processing capabilities which are used in a new concept of automated in situ gas quantification by photo-optical detection. The design for an in situ high-speed image acquisition and automated data processing system is reported ("Bubblemeter". New strategies have been followed with regards to back-light illumination, bubble extraction, automated image processing and data management. This paper presents the design of the novel method, its validation procedures and calibration experiments. The system will be positioned and recovered from the sea floor using a remotely operated vehicle (ROV. It is able to measure bubble flux rates up to 10 L/min with a maximum error of 33% for worst case conditions. The Bubblemeter has been successfully deployed at a water depth of 1023 m at the Makran accretionary prism offshore Pakistan during a research expedition with R/V Meteor in November 2007.
In situ deposition of thallium-containing oxides
International Nuclear Information System (INIS)
Myers, K.E.
1996-01-01
The number and variety of thallium based materials that can be made by in situ methods have grown consistently since the first report of successful thallium cuprate deposition by Face and Nestlerode in 1992. Processes for the deposition of superconductors, normal metals, and insulators have been developed. Most work to date has been done on the Tl-1212 phases, TlBa 2 CaCu 2 O 7 and (Tl,Pb)Sr 2 CaCu 2 O 7 . Recently however, the in situ thallium technique has been extended to other materials. For example, epitaxial thin films of thallium tantalate, an insulator of the pyrochlore structure and a potential buffer layer for thallium cuprate films, have been grown. Multilayers, important in the fabrication of Josephson junctions, have been demonstrated with the thallium lead cuprates. This paper reviews progress in the area of in situ thallium deposition technology which will make more complex thallium cuprate multilayer structures and devices possible
Laboratory-performance criteria for in situ waste-stabilization materials
International Nuclear Information System (INIS)
Shaw, P.; Weidner, J.
1996-01-01
The Department of Energy (DOE) Landfill Stabilization Focus Area is investigating a variety of in situ placement methods, grout materials, and characterization techniques for the stabilization of buried low-level transuranic-contaminated waste at Department of Energy sites. In situ stabilization involves underground injection or placement of substances to isolate, treat, or contain buried contaminants. Performance criteria were developed to evaluate various candidate stabilization materials for both long-term stabilization and interim stabilization or retrieval. The criteria are go/no-go, ready, and preliminary. The criterion go/no-go eliminates technologies that are not applicable for in situ treatment of buried waste. The criterion ready indicates that the technology is sufficiently developed and proven to be field demonstrated full-scale. The criterion preliminary indicates the prospective technologies to be potentially applicable to in situ buried waste stabilization, but further development is needed before the technology is ready for field-scale demonstration
Gawlak, M; Górkiewicz, T; Gorlewicz, A; Konopacki, F A; Kaczmarek, L; Wilczynski, G M
2009-01-12
Synaptic plasticity involves remodeling of extracellular matrix. This is mediated, in part, by enzymes of the matrix metalloproteinase (MMP) family, in particular by gelatinase MMP-9. Accordingly, there is a need of developing methods to visualize gelatinolytic activity at the level of individual synapses, especially in the context of neurotransmitters receptors. Here we present a high-resolution fluorescent in situ zymography (ISZ), performed in thin sections of the alcohol-fixed and polyester wax-embedded brain tissue of the rat (Rattus norvegicus), which is superior to the current ISZ protocols. The method allows visualization of structural details up to the resolution-limit of light microscopy, in conjunction with immunofluorescent labeling. We used this technique to visualize and quantify gelatinolytic activity at the synapses in control and seizure-affected rat brain. In particular, we demonstrated, for the first time, frequent colocalization of gelatinase(s) with synaptic N-methyl-D-aspartic acid (NMDA)- and AMPA-type glutamate receptors. We believe that our method represents a valuable tool to study extracellular proteolytic processes at the synapses, it could be used, as well, to investigate proteinase involvement in a range of physiological and pathological phenomena in the nervous system.
Bleckmann, Andrea; Dresselhaus, Thomas
2016-04-01
First evidence on gene function and regulation is provided by the cellular expression pattern in complex tissues. However, to understand the activity of a specific gene, it is essential to analyze the regulatory network, which controls the spatio-temporal translation pattern during the entire life span of the transcribed mRNA. To explore mechanisms which control mRNA abundance and localization in space and time, it is necessary to visualize mRNAs quantitatively with a subcellular resolution, without sectioning the tissues. We have adapted and optimized a protocol for colorimetric whole-mount RNA in situ hybridization (WISH) using egg cell-specific digoxigenin (DIG) labeled probes (Hejátko et al., 2006) [1] on ovules and early seeds of Arabidopsis. Furthermore, we established a fluorescent whole-mount RNA in situ hybridization (F-WISH) protocol, which allows mRNA visualization on a subcellular level. The polar localized mRNA of SBT4.13, encoding a subtilase, was identified using this protocol. Both methods are described and discussed in detail. Additionally a (F)-WISH flow-chart is provided along with a troubleshooting table. Copyright © 2015 Elsevier Inc. All rights reserved.
Model and calculation of in situ stresses in anisotropic formations
Energy Technology Data Exchange (ETDEWEB)
Yuezhi, W.; Zijun, L.; Lixin, H. [Jianghan Petroleum Institute, (China)
1997-08-01
In situ stresses in transversely isotropic material in relation to wellbore stability have been investigated. Equations for three horizontal in- situ stresses and a new formation fracture pressure model were described, and the methodology for determining the elastic parameters of anisotropic rocks in the laboratory was outlined. Results indicate significantly smaller differences between theoretically calculated pressures and actual formation pressures than results obtained by using the isotropic method. Implications for improvements in drilling efficiency were reviewed. 13 refs., 6 figs.
In Situ High Resolution Synchrotron X-Ray Powder Diffraction Studies of Lithium Batteries
DEFF Research Database (Denmark)
Amri, Mahrez; Fitch, Andy; Norby, Poul
2015-01-01
allowing diffraction information to be obtained from only the active material during battery operation [2]. High resolution synchrotron x-ray powder diffraction technique has been undertaken to obtain detailed structural and compositional information during lithiation/delithiation of commercial LiFePO4...... materials [3]. We report results from the first in situ time resolved high resolution powder diffraction experiments at beamline ID22/31 at the European Synchrotron Radiation Facility, ESRF. We follow the structural changes during charge of commercial LiFePO4 based battery materials using the Rietveld...... method. Conscientious Rietveld analysis shows slight but continuous deviation of lattice parameters from those of the fully stoichiometric end members LiFePO4 and FePO4 indicating a subsequent variation of stoichiometry during cathode delithiation. The application of an intermittent current pulses during...
Oxygen-free in situ scanning tunnelling microscopy
DEFF Research Database (Denmark)
Zhang, Jingdong; Ulstrup, Jens
2007-01-01
Scanning tunneling microscopy under full electrochemical potential control (in situ STM) has been used extensively as an efficient method to characterize microstructures at solid/liquid interfaces at the atomic and molecular levels. However, under ambient conditions oxygen may interfere in open i...
One ligand capable of in situ reaction in a mixed-ligand system with two new different frameworks
Wang, Xiaofang
2017-12-24
The in situ ligand 2,3-pyrazinedicarboxylic acid (2,3-H2pzdc) mixed with 1,1′-(1,4-butanediyl)bis(benzimidazole) (bbbi) is used to form two coordination polymers ([Cd(2,3-pzdc)(bbbi)] (1) and [Cd2Cl3(2-pzc)(bbbi)2] (2)) under hydrothermal conditions. Complex 1 was obtained in the absence of in situ reaction and 2 was synthesized with 2,3-H2pzdc in situ generating 2-pyrazinecarboxylate (2-pzc−). The structural details reveal that 1 has a 3D framework with dia topology, and 2 is a 2D layer structure and develops a 3D supramolecular structure via strong π⋯π stacking interactions. The ligand effects were compared for the two frameworks. In addition, fluorescence properties and thermal stabilities of 1 and 2 in the solid were studied.
DEFF Research Database (Denmark)
Spataru, Sergiu; Hacke, Peter; Sera, Dezso
2015-01-01
We propose a method of in-situ characterization of the photovoltaic module power at standard test conditions using superposition of the dark current-voltage (I-V) curve measured at elevated stress temperature during potential-induced degradation (PID) testing. PID chamber studies were performed o...
Effect of In-Situ Curing on Compressive Strength of Reactive Powder Concrete
Directory of Open Access Journals (Sweden)
Bali Ika
2016-01-01
Full Text Available A development of Reactive Powder Concrete (RPC currently is the use of quartz powder as a stabilizing agent with the content to cement ratio of 30% and steam curing method in an autoclave temperature of 250ºC which produced a high compressive strength of 180 MPa. That RPC can be generated due to one reason for using the technique of steam curing in an autoclave in the laboratory. This study proposes in-situ curing method in order the curing can be applied in the field and with a reasonable compressive strength results of RPC. As the benchmarks in this study are the curing methods in laboratory that are steam curing of 90°C for 8 hours (C1, and water curing for 28 days (C2. For the in-situ curing methods that are covering with tarpaulins and flowed steam of 3 hours per day for 7 days (C3, covering with wet sacks for 28 days (C4, and covering with wet sacks for 28 days for specimen with unwashed sand as fine aggregate (C5. The comparison of compressive strength of the specimens in this study showed compressive strength of RPC with in-situ steam curing (101.64 MPa close to the compressive strength of RPC with steam curing in the laboratory with 8.2% of different. While in-situ wet curing compared with the water curing in laboratory has the different of 3.4%. These results indicated that the proposed in-situ curing methods are reasonable good in term of the compressive strength that can be achieved.
International Nuclear Information System (INIS)
Evans, D.D.
1992-01-01
In-situ burning of spilled oil has distinct advantages over other countermeasures. It offers the potential to convert rapidly large quantities of oil into its primary combustion products, carbon dioxide and water, with a small percentage of other unburned and residue byproducts. Because the oil is converted to gaseous products of combustion by burning, the need for physical collection, storage, and transport of recovered fluids is reduced to the few percent of the original spill volume that remains as residue after burning. Burning oil spills produces a visible smoke plume containing smoke particulate and other products of combustion which may persist for many kilometers from the burn. This fact gives rise to public health concerns, related to the chemical content of the smoke plume and the downwind deposition of particulate, which need to be answered. In 1985, a joint Minerals Management Service (MMS) and Environment Canada (EC) in-situ burning research program was begun at the National Institute of Standards and Technology (NIST). This research program was designed to study the burning of large crude oil spills on water and how this burning would affect air quality by quantifying the products of combustion and developing methods to predict the downwind smoke particulate deposition. To understand the important features of in-situ burning, it is necessary to perform both laboratory and mesoscale experiments. Finally, actual burns of spilled oil at sea will be necessary to evaluate the method at the anticipated scale of actual response operations. In this research program there is a continuing interaction between findings from measurements on small fire experiments performed in the controlled laboratory environments of NIST and the Fire Research Institute (FRI) in Japan, and large fire experiments at facilities like the USCG Fire Safety and Test Detachment in Mobile, Alabama where outdoor liquid fuel burns in large pans are possible
RNA Imaging with Multiplexed Error Robust Fluorescence in situ Hybridization
Moffitt, Jeffrey R.; Zhuang, Xiaowei
2016-01-01
Quantitative measurements of both the copy number and spatial distribution of large fractions of the transcriptome in single-cells could revolutionize our understanding of a variety of cellular and tissue behaviors in both healthy and diseased states. Single-molecule Fluorescence In Situ Hybridization (smFISH)—an approach where individual RNAs are labeled with fluorescent probes and imaged in their native cellular and tissue context—provides both the copy number and spatial context of RNAs but has been limited in the number of RNA species that can be measured simultaneously. Here we describe Multiplexed Error Robust Fluorescence In Situ Hybridization (MERFISH), a massively parallelized form of smFISH that can image and identify hundreds to thousands of different RNA species simultaneously with high accuracy in individual cells in their native spatial context. We provide detailed protocols on all aspects of MERFISH, including probe design, data collection, and data analysis to allow interested laboratories to perform MERFISH measurements themselves. PMID:27241748
In Situ X-ray Diffraction Studies of Cathode Materials in Lithium Batteries
International Nuclear Information System (INIS)
Yang, X. Q.; Sun, X.; McBreen, J.; Mukerjee, S.; Gao, Yuan; Yakovleva, M. V.; Xing, X. K.; Daroux, M. L.
1998-01-01
There is an increasing interest in lithiated transition metal oxides because of their use as cathodes in lithium batteries. LiCoO 2 , LiNiO 2 and LiMn 2 O 4 are the three most widely used and studied materials, At present, although it is relative expensive and toxic, LiCoO 2 is the material of choice in commercial lithium ion batteries because of its ease of manufacture, better thermal stability and cycle life. However, the potential use of lithium ion batteries with larger capacity for power tools and electric vehicles in the future will demand new cathode materials with higher energy density, lower cost and better thermal stability. LiNiO 2 is isostructural with LiCoO 2 . It offers lower cost and high energy density than LiCoO 2 . However, it has much poorer thermal stability than LiCoO 2 , in the charged (delithiated) state. Co, Al, and other elements have been used to partially replace Ni in LiNiO 2 system in order to increase the thermal stability. LiMn 2 O 4 has the highest thermal stability and lowest cost and toxicity. However, the low energy density and poor cycle life at elevated temperature are the major obstacles for this material. In order to develop safer, cheaper, and better performance cathode materials, the in-depth understanding of the relationships between the thermal stability and structure, performance and structure are very important. The performance here includes energy density and cycle life of the cathode materials. X-ray diffraction (XRD) is one of the most powerful tools to study these relationships. The pioneer ex situ XRD work on cathode materials for lithium batteries was done by Ohzuku. His XRD studies on LiMn 2 O 4 , LiCoO 2 , LiNiO 2 , LiNi 0.5 Co 0.5 O 2 , and LiAl x Ni 1-x O 2 cathodes at different states of charge have provided important guidelines for the development of these new materials. However, the kinetic nature of the battery system definitely requires an in situ XRD technique to study the detail structural changes of the
Application of in situ diffraction in high-throughput structure determination platforms.
Aller, Pierre; Sanchez-Weatherby, Juan; Foadi, James; Winter, Graeme; Lobley, Carina M C; Axford, Danny; Ashton, Alun W; Bellini, Domenico; Brandao-Neto, Jose; Culurgioni, Simone; Douangamath, Alice; Duman, Ramona; Evans, Gwyndaf; Fisher, Stuart; Flaig, Ralf; Hall, David R; Lukacik, Petra; Mazzorana, Marco; McAuley, Katherine E; Mykhaylyk, Vitaliy; Owen, Robin L; Paterson, Neil G; Romano, Pierpaolo; Sandy, James; Sorensen, Thomas; von Delft, Frank; Wagner, Armin; Warren, Anna; Williams, Mark; Stuart, David I; Walsh, Martin A
2015-01-01
Macromolecular crystallography (MX) is the most powerful technique available to structural biologists to visualize in atomic detail the macromolecular machinery of the cell. Since the emergence of structural genomics initiatives, significant advances have been made in all key steps of the structure determination process. In particular, third-generation synchrotron sources and the application of highly automated approaches to data acquisition and analysis at these facilities have been the major factors in the rate of increase of macromolecular structures determined annually. A plethora of tools are now available to users of synchrotron beamlines to enable rapid and efficient evaluation of samples, collection of the best data, and in favorable cases structure solution in near real time. Here, we provide a short overview of the emerging use of collecting X-ray diffraction data directly from the crystallization experiment. These in situ experiments are now routinely available to users at a number of synchrotron MX beamlines. A practical guide to the use of the method on the MX suite of beamlines at Diamond Light Source is given.
A mass conserving level set method for detailed numerical simulation of liquid atomization
Energy Technology Data Exchange (ETDEWEB)
Luo, Kun; Shao, Changxiao [State Key Laboratory of Clean Energy Utilization, Zhejiang University, Hangzhou 310027 (China); Yang, Yue [State Key Laboratory of Turbulence and Complex Systems, Peking University, Beijing 100871 (China); Fan, Jianren, E-mail: fanjr@zju.edu.cn [State Key Laboratory of Clean Energy Utilization, Zhejiang University, Hangzhou 310027 (China)
2015-10-01
An improved mass conserving level set method for detailed numerical simulations of liquid atomization is developed to address the issue of mass loss in the existing level set method. This method introduces a mass remedy procedure based on the local curvature at the interface, and in principle, can ensure the absolute mass conservation of the liquid phase in the computational domain. Three benchmark cases, including Zalesak's disk, a drop deforming in a vortex field, and the binary drop head-on collision, are simulated to validate the present method, and the excellent agreement with exact solutions or experimental results is achieved. It is shown that the present method is able to capture the complex interface with second-order accuracy and negligible additional computational cost. The present method is then applied to study more complex flows, such as a drop impacting on a liquid film and the swirling liquid sheet atomization, which again, demonstrates the advantages of mass conservation and the capability to represent the interface accurately.
In Situ Local Contact Angle Measurement in a CO2-Brine-Sand System Using Microfocused X-ray CT.
Lv, Pengfei; Liu, Yu; Wang, Zhe; Liu, Shuyang; Jiang, Lanlan; Chen, Junlin; Song, Yongchen
2017-04-11
The wettability of porous media is of major interest in a broad range of natural and engineering applications. The wettability of a fluid on a solid surface is usually evaluated by the contact angle between them. While in situ local contact angle measurements are complicated by the topology of porous media, which can make it difficult to use traditional methods, recent advances in microfocused X-ray computed tomography (micro-CT) and image processing techniques have made it possible to measure contact angles on the scale of the pore sizes in such media. However, the effects of ionic strength, CO 2 phase, and flow pattern (drainage or imbibition) on pore-scale contact angle distribution are still not clear and have not been reported in detail in previous studies. In this study, we employed a micro-CT scanner for in situ investigation of local contact angles in a CO 2 -brine-sand system under various conditions. The effects of ionic strength, CO 2 phase, and flow pattern on the local contact-angle distribution were examined in detail. The results showed that the local contact angles vary over a wide range as a result of the interaction of surface contaminants, roughness, pore topology, and capillarity. The wettability of a porous surface could thus slowly weaken with increasing ionic strength, and the average contact angle could significantly increase when gaseous CO 2 (gCO 2 ) turns into supercritical CO 2 (scCO 2 ). Contact angle hysteresis also occurred between drainage and imbibition procedures, and the hysteresis was more significant under gCO 2 condition.
In Situ Remediation Integrated Program: Technology summary
International Nuclear Information System (INIS)
1994-02-01
The In Situ Remediation Integrated Program (ISR IP) was instituted out of recognition that in situ remediation could fulfill three important criteria: significant cost reduction of cleanup by eliminating or minimizing excavation, transportation, and disposal of wastes; reduced health impacts on workers and the public by minimizing exposure to wastes during excavation and processing; and remediation of inaccessible sites, including: deep subsurfaces, in, under, and around buildings. Buried waste, contaminated soils and groundwater, and containerized wastes are all candidates for in situ remediation. Contaminants include radioactive wastes, volatile and non-volatile organics, heavy metals, nitrates, and explosive materials. The ISR IP intends to facilitate development of in situ remediation technologies for hazardous, radioactive, and mixed wastes in soils, groundwater, and storage tanks. Near-term focus is on containment of the wastes, with treatment receiving greater effort in future years. ISR IP is an applied research and development program broadly addressing known DOE environmental restoration needs. Analysis of a sample of 334 representative sites by the Office of Environmental Restoration has shown how many sites are amenable to in situ remediation: containment--243 sites; manipulation--244 sites; bioremediation--154 sites; and physical/chemical methods--236 sites. This needs assessment is focused on near-term restoration problems (FY93--FY99). Many other remediations will be required in the next century. The major focus of the ISR EP is on the long term development of permanent solutions to these problems. Current needs for interim actions to protect human health and the environment are also being addressed
In Situ Remediation Integrated Program: Technology summary
Energy Technology Data Exchange (ETDEWEB)
1994-02-01
The In Situ Remediation Integrated Program (ISR IP) was instituted out of recognition that in situ remediation could fulfill three important criteria: significant cost reduction of cleanup by eliminating or minimizing excavation, transportation, and disposal of wastes; reduced health impacts on workers and the public by minimizing exposure to wastes during excavation and processing; and remediation of inaccessible sites, including: deep subsurfaces, in, under, and around buildings. Buried waste, contaminated soils and groundwater, and containerized wastes are all candidates for in situ remediation. Contaminants include radioactive wastes, volatile and non-volatile organics, heavy metals, nitrates, and explosive materials. The ISR IP intends to facilitate development of in situ remediation technologies for hazardous, radioactive, and mixed wastes in soils, groundwater, and storage tanks. Near-term focus is on containment of the wastes, with treatment receiving greater effort in future years. ISR IP is an applied research and development program broadly addressing known DOE environmental restoration needs. Analysis of a sample of 334 representative sites by the Office of Environmental Restoration has shown how many sites are amenable to in situ remediation: containment--243 sites; manipulation--244 sites; bioremediation--154 sites; and physical/chemical methods--236 sites. This needs assessment is focused on near-term restoration problems (FY93--FY99). Many other remediations will be required in the next century. The major focus of the ISR EP is on the long term development of permanent solutions to these problems. Current needs for interim actions to protect human health and the environment are also being addressed.
DEFF Research Database (Denmark)
Svendsen, Claus Bo; Boye, Mette; Struve, Carsten
2009-01-01
A novel, sensitive and specific method for detecting Rickettsia spp. in archival samples is described. The method involves the use of fluorescently marked oligonucleotide probes for in situ hybridization. Specific hybridization of Ricekttsia was found without problems of cross-reactions with bact......A novel, sensitive and specific method for detecting Rickettsia spp. in archival samples is described. The method involves the use of fluorescently marked oligonucleotide probes for in situ hybridization. Specific hybridization of Ricekttsia was found without problems of cross...
In situ rheology of yeast biofilms.
Brugnoni, Lorena I; Tarifa, María C; Lozano, Jorge E; Genovese, Diego
2014-01-01
The aim of the present work was to investigate the in situ rheological behavior of yeast biofilms growing on stainless steel under static and turbulent flow. The species used (Rhodototula mucilaginosa, Candida krusei, Candida kefyr and Candida tropicalis) were isolated from a clarified apple juice industry. The flow conditions impacted biofilm composition over time, with a predominance of C. krusei under static and turbulent flow. Likewise, structural variations occurred, with a tighter appearance under dynamic flow. Under turbulent flow there was an increase of 112 μm in biofilm thickness at 11 weeks (p < 0.001) and cell morphology was governed by hyphal structures and rounded cells. Using the in situ growth method introduced here, yeast biofilms were determined to be viscoelastic materials with a predominantly solid-like behavior, and neither this nor the G'0 values were significantly affected by the flow conditions or the growth time, and at large deformations their weak structure collapsed beyond a critical strain of about 1.5-5%. The present work could represent a starting point for developing in situ measurements of yeast rheology and contribute to a thin body of knowledge about fungal biofilm formation.
Application of in situ x-ray diffraction techniques in heterogenous catalytic systems
International Nuclear Information System (INIS)
Sharifah Bee Abd Hamid
2002-01-01
A broad range of techniques is available today for the characterisation of catalysts and the investigation of catalyst reaction mechanisms. However, only a limited number of those are suitable for in situ studies, i.e experiments performed in conditions mimicking or close as possible to real operating conditions. Various commercially and in-house developed in situ X-Ray diffraction (XRD) cells have been used to obtain information on the phase and structure of materials at the initial formation stage, activation methodology, calcination, reduction and carburization. A major advantage of the in situ X-ray cells is that it allows direct observations on the decomposition of precursors leading to various phases in a controlled environment, i.e. controlled temperature and pressure under specified gases. The cells can be operated both at high temperatures and high pressures, equipped with Position Sensitive Detector (PSD), feature which was used to study phase transformation occurring during the activation of various solids. In MoO 3 , XRD results provide detailed information on the hydrogen insertion into its lattice, followed by carburization providing good understanding on the mechanism in the solid transformation leading to the metastable MoC 1 -x phase. For the Bi-SnO x systems, the environmental cell coupled with XRD and PSD allow the design of activation procedure to obtain the active Bi 2 Sn 2 O 7 . The in situ XRD technique reveals crucial information on the initial stage of oxides formations prior to condensation reaction shown in MCM-41 and titania systems. In this presentation, discussions on general achievements and problems relating to the use of in situ XRD techniques as well as of specific examples selected to illustrate the use and potential of in situ XRD are made. It is not intended to be a review of the art but a highlight of the challenges which the catalytic and material scientists face when entering the avenue. (Author)
Energy Technology Data Exchange (ETDEWEB)
Labbaye, T.; Gaillard, M.; Lecas, T.; Kovacevic, E.; Boulmer-Leborgne, Ch.; Guimbretière, G. [GREMI, Université-CNRS, BP6744, 45067 Orléans Cedex 2 (France); Canizarès, A.; Raimboux, N.; Simon, P.; Ammar, M. R., E-mail: mohamed-ramzi.ammar@cnrs-orleans.fr [CNRS, CEMHTI UPR3079, Univ. Orléans, F-45071 Orléans Cedex 2 (France); Strunskus, T. [Institute of Material Science, Chritian-Albrechts-University of Kiel, D-24143 Kiel (Germany)
2014-11-24
Portable and highly sensitive Raman setup was associated with a plasma-enhanced chemical vapor deposition reactor enabling in situ growth monitoring of multi-wall carbon nanotubes despite the combination of huge working distance, high growth speed and process temperature and reactive plasma condition. Near Edge X-ray absorption fine structure spectroscopy was used for ex situ sample analysis as a complementary method to in situ Raman spectroscopy. The results confirmed the fact that the “alternating” method developed here can accurately be used for in situ Raman monitoring under reactive plasma condition. The original analytic tool can be of great importance to monitor the characteristics of these nanostructured materials and readily define the ultimate conditions for targeted results.
OpenMC In Situ Source Convergence Detection
Energy Technology Data Exchange (ETDEWEB)
Aldrich, Garrett Allen [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Univ. of California, Davis, CA (United States); Dutta, Soumya [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); The Ohio State Univ., Columbus, OH (United States); Woodring, Jonathan Lee [Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
2016-05-07
We designed and implemented an in situ version of particle source convergence for the OpenMC particle transport simulator. OpenMC is a Monte Carlo based-particle simulator for neutron criticality calculations. For the transport simulation to be accurate, source particles must converge on a spatial distribution. Typically, convergence is obtained by iterating the simulation by a user-settable, fixed number of steps, and it is assumed that convergence is achieved. We instead implement a method to detect convergence, using the stochastic oscillator for identifying convergence of source particles based on their accumulated Shannon Entropy. Using our in situ convergence detection, we are able to detect and begin tallying results for the full simulation once the proper source distribution has been confirmed. Our method ensures that the simulation is not started too early, by a user setting too optimistic parameters, or too late, by setting too conservative a parameter.
International Nuclear Information System (INIS)
Lajudie, A.; Coulon, H.; Geneste, P.
1991-01-01
Knowledge of deep formation for radioactive waste disposal requires field-tests or bench-scale experiments on samples of the site material. In the case of clay massifs the taking of cores and the sampling of these are particularly difficult. The most suitable materials and techniques were selected from a study of clay colling and conservation methods. These were used for a series of core samples taken at Mol in Belgium. Subsequently permeability measurements were carried out in laboratory on samples from vertical drilling and compared with in situ measurements. The latter were made by horizontal drillings from the shaft excavation of the underground facility HADES at Mol. There is a good overall agreement between the results of the two types of measurements. 25 figs.; 4 tabs.; 12 refs.; 16 photos
Atoms in Action: Observing Atomic Motion with Dynamic in situ X-ray Diffraction
Cox, Jordan Michael
Metal-organic framework (MOF) materials are rich in both structural diversity and application. These materials are comprised of metal atoms or clusters which are connected in a three-dimensional polymer-like network by bridging organic linker molecules. One of the major attractive features in MOFs is their permanent pore space which can potentially be used to adsorb or exchange foreign molecules from/with the surrounding environment. While MOFs are an active area of scientific interest, MOF materials are still relatively new, only 20 years old. As such, there is still much that needs to be understood about these materials before they can be effectively applied to widespread chemical problems like CO2 sequestration or low-pressure hydrogen fuel storage. One of the most important facets of MOF chemistry to understand in order to rationally design MOF materials with tailor-made properties is the relationship between the structural features in a MOF and the chemical and physical properties of that material. By examining in detail the atomic structure of a MOF with known properties under a variety of conditions, scientists can begin to unravel the guiding principles which govern these relationships. X-ray diffraction remains one of the most effective tools for determining the structure of a crystalline material with atomic resolution, and has been applied to the determination of MOF structures for years. Typically these experiments have been carried out using powder X-ray diffraction, but this technique lacks the high-resolution structural information found in single-crystal methods. Some studies have been reported which use specialized devices, sometimes called Environmental Control Cells, to study single crystalline MOFs under non-ambient chemical conditions in situ . However, these in situ studies are performed under static conditions. Even in cases where the ECC provides continued access to the local chemical environment during diffraction data collections, the
In-situ failure test in the research tunnel at Olkiluoto
Energy Technology Data Exchange (ETDEWEB)
Autio, J.; Johansson, E.; Kirkkomaeki, T. [Saanio and Riekkola Consulting Engineers, Helsinki (Finland); Hakala, M. [Gridpoint Finland Oy (Finland); Heikkilae, E. [Helsinki Univ. of Technology, Otaniemi (Finland). Lab. of Rock Engineering
2000-05-01
A failure test suitable for execution in the Research Tunnel at Olkiluoto has been planned to study the failure of rock in-situ. The objectives of the in-situ failure test is to assess the applicability of numerical modelling codes and methods to the study of rock failure and associated crack propagation and to develop a novel technique to be used to determine the strength of rock in-situ. The objective of this study was to make a preliminary design of the failure test, assess the technical feasibility of the test and to give input information for further numerical modelling of the test. The design of the failure test is reported and results of preliminary modelling are given. The input information for future modelling includes a study of rock properties, fracture propagation in rock, in-situ stresses and the development of techniques for using the expanding agent to produce artificial stress field. The study showed that mechanical properties such as strength of gneissic tonalite, the main rock type in the Research Tunnel, depends highly on the orientation of schistocity. The in-situ failure test was shown to be technically feasible and a state of stress high enough to cause failure can be created artificially by using a proper expansive agent and design. (orig.)
Very little in situ produced radiocarbon retained in accumulating Antarctic ice
International Nuclear Information System (INIS)
Kemp, W.J.M. van der; Alderliesten, C.; Borg, K. van der; Holmlund, P.; Jong, A.F.M. de; Karloef, L.; Lamers, R.A.N.; Oerlemans, J.; Thomassen, M.; Wal, R.S.W. van de
2000-01-01
Ice samples from Dronning Maud Land, Antarctica, were analyzed for 14 CO 2 and 14 CO by accelerator mass spectrometry. Only a small amount (∼2%) of in situ produced radiocarbon was detected. The calibrated radiocarbon ages, corrected for in situ produced 14 C, are in fair agreement with age estimates obtained from stratigraphical methods added to a gas inclusion model. The ages of the entrapped air range from recent to ca. 1200 AD
Contemporary management of ductal carcinoma in situ and lobular carcinoma in situ.
Obeng-Gyasi, Samilia; Ong, Cecilia; Hwang, E Shelley
2016-06-01
The management of in situ lesions ductal carcinoma in situ (DCIS) and lobular carcinoma in situ (LCIS) continues to evolve. These diagnoses now comprise a large burden of mammographically diagnosed cancers, and with a global trend towards more population-based screening, the incidence of these lesions will continue to rise. Because outcomes following treatment for DCIS and LCIS are excellent, there is emerging controversy about what extent of treatment is optimal for both diseases. Here we review the current approaches to the diagnosis and treatment of both DCIS and LCIS. In addition, we will consider potential directions for future management of these lesions.
SR-Can. Data and uncertainty assessment. Matrix diffusivity and porosity in situ
International Nuclear Information System (INIS)
Jinsong Liu; Loefgren, Martin; Neretnieks, Ivars
2006-12-01
and show beyond doubt that the electrical conductivity method using AC gives the expected information on transport properties (diffusivities) of the pores of crystalline rocks. The electrical conductivity method is much faster and can be used to measure large samples. It has recently been adopted for use in deep boreholes. Tens of thousands of measurements have been made at Simpevarp, Laxemar and Forsmark (Swedish sites) at depths of more than 1,000 m. The results of these measurements form the basis for our proposed diffusion values to be used in Performance Assessment (PA) for the candidate sites (Performance Assessment (PA) used in this report is synonymous to Safety Assessment (SA) sometimes used by other authors). The in situ data are obtained essentially in undisturbed rock and have not been subject to either stress release or disturbances due to sample preparation. The small disturbance nearest the borehole is negligible because the electrical conductivity method samples rock extending to more than a metre from the borehole. A large number of laboratory measurements have been analysed in order to ensure that other effects that cannot be controlled in the in situ measurements do not influence the down-hole data. No unexpected effects have been found. Rock matrix porosity in situ measurements are extremely scarce. However, it has been possible to use some of the in situ measurements to estimate the increase in porosity when taking up rock from its natural environment to the laboratory. One example of such an investigation is briefly discussed to show how this was done. In one in situ diffusion experiment performed at a depth of 60 m in granitic rock in Sweden the experimental conditions were such that it was ensured that any rock stress changes due to the presence of the drift and the presence of the borehole were avoided. The rock was thus subject to 'virgin stress'. Over-coring after exposure to tracers for three and a half years and detailed sampling and
SR-Can. Data and uncertainty assessment. Matrix diffusivity and porosity in situ
Energy Technology Data Exchange (ETDEWEB)
Jinsong Liu; Loefgren, Martin; Neretnieks, Ivars [Dept. of Chemical Engineering and Technology, Royal Inst. of Technology, Stockholm (Sweden)
2006-12-15
and show beyond doubt that the electrical conductivity method using AC gives the expected information on transport properties (diffusivities) of the pores of crystalline rocks. The electrical conductivity method is much faster and can be used to measure large samples. It has recently been adopted for use in deep boreholes. Tens of thousands of measurements have been made at Simpevarp, Laxemar and Forsmark (Swedish sites) at depths of more than 1,000 m. The results of these measurements form the basis for our proposed diffusion values to be used in Performance Assessment (PA) for the candidate sites (Performance Assessment (PA) used in this report is synonymous to Safety Assessment (SA) sometimes used by other authors). The in situ data are obtained essentially in undisturbed rock and have not been subject to either stress release or disturbances due to sample preparation. The small disturbance nearest the borehole is negligible because the electrical conductivity method samples rock extending to more than a metre from the borehole. A large number of laboratory measurements have been analysed in order to ensure that other effects that cannot be controlled in the in situ measurements do not influence the down-hole data. No unexpected effects have been found. Rock matrix porosity in situ measurements are extremely scarce. However, it has been possible to use some of the in situ measurements to estimate the increase in porosity when taking up rock from its natural environment to the laboratory. One example of such an investigation is briefly discussed to show how this was done. In one in situ diffusion experiment performed at a depth of 60 m in granitic rock in Sweden the experimental conditions were such that it was ensured that any rock stress changes due to the presence of the drift and the presence of the borehole were avoided. The rock was thus subject to 'virgin stress'. Over-coring after exposure to tracers for three and a half years and detailed sampling
Styrene grafted natural rubber reinforced by in situ silica generated via sol–gel technique
Energy Technology Data Exchange (ETDEWEB)
Sittiphan, Torpong [Program of Petrochemistry and Polymer Sciences, Faculty of Science, Chulalongkorn University, Bangkok 10330 (Thailand); Prasassarakich, Pattarapan [Department of Chemical Technology, Faculty of Science, Chulalongkorn University, Bangkok 10330 (Thailand); Poompradub, Sirilux, E-mail: sirilux.p@chula.ac.th [Department of Chemical Technology, Faculty of Science, Chulalongkorn University, Bangkok 10330 (Thailand)
2014-02-15
Highlights: • Sol–gel reaction by NR latex was the absence of use of organic solvent and base catalyst. • Well dispersed in situ formed silica particles in the rubber matrix were obtained. • In situ silica was better to improve mechanical properties of rubber vulcanizates. -- Abstract: The filling of styrene graft natural rubber (ST-GNR) with in situ formed silica was performed using the sol–gel reaction via the latex solution method. The mechanical properties of ST-GNR/NR vulcanizate were improved when using the in situ formed silica to levels higher than those obtained with the commercial ex situ formed silica filled ST-GNR/NR vulcanizates at a comparable silica content of 12 parts by weight per hundred parts of rubber. Transmission electron microscopy analysis revealed that the in situ silica particles were small (∼40 nm diameter) and well dispersed, while the commercial silica particles were larger (∼60 nm diameter) and markedly agglomerated in the rubbery matrix. The mechanical properties of the composites prepared via both the solid rubber and latex solution methods were comparable.
Structural Investigation of Sodium Layered Oxides Via in Situ Synchrotron X-Ray Diffraction
DEFF Research Database (Denmark)
Jung, Young Hwa; Christiansen, Ane Sælland; Johnsen, Rune
2015-01-01
electrochemical reaction is generally considered to be a pivotal feature for understanding the relationship between layered structures and electrochemical properties. Here the structure, phase stability, and electrochemical properties of two kinds of layered oxides, P2 and O3, are investigated through in......-situ synchrotron XRD experiments. A capillary Na-based cell is designed to minimize interference in other substances such as a separator or external battery parts. This approach could give us to obtain clear diffraction patterns with high intensity during electrochemical reaction in a short period of time without...... further relaxation step. We carefully scrutinized reversible structural phase transformations during electrochemical reaction of P2 and O3-layered compounds based on in situ analysis, and detailed results will be discussed....
A microfluidic membrane chip for in situ fouling characterization
Ngene, I.S.; Lammertink, Rob G.H.; Wessling, Matthias; van der Meer, Walterus Gijsbertus Joseph
2010-01-01
A new method for non-invasive in situ monitoring of a microfiltration process is described. In microfiltration systems, local information on the deposition characteristics can be used to determine the cake behavior during a filtration run. Typically, non-invasive methods of fouling study are
Method and Circuit for In-Situ Health Monitoring of Solar Cells in Space
Krasowski, Michael J.; Prokop, Norman F.
2010-01-01
This innovation represents a method and circuit realization of a system designed to make in-situ measurements of test solar-cell operational parameters on orbit using readily available high-temperature and high-ionizing-radiation- tolerant electronic components. This innovation enables on-orbit in-situ solar-array health monitoring and is in response to a need recognized by the U.S. Air Force for future solar arrays for unmanned spacecraft. This system can also be constructed out of commercial-grade electronics and can be embedded into terrestrial solar power system as a diagnostics instrument. This innovation represents a novel approach to I-V curve measurement that is radiation and temperature hard, consumes very few system resources, is economical, and utilizes commercially available components. The circuit will also operate at temperatures as low as 55 C and up to +225 C, allowing it to reside close to the array in direct sunlight. It uses a swept mode transistor functioning as a resistive load while utilizing the solar cells themselves as the biasing device, so the size of the instrument is small and there is no danger of over-driving the cells. Further, this innovation utilizes nearly universal spacecraft bus resources and therefore can be readily adapted to any spacecraft bus allowing for ease of retrofit, or designed into new systems without requiring the addition of infrastructure. One unique characteristic of this innovation is that it effects the measurement of I-V curves without the use of large resistor arrays or active current sources normally used to characterize cells. A single transistor is used as a variable resistive load across the cell. This multi-measurement instrument was constructed using operational amplifiers, analog switches, voltage regulators, MOSFETs, resistors, and capacitors. The operational amplifiers, analog switches, and voltage regulators are silicon-on-insulator (SOI) technology known for its hardness to the effects of ionizing
In situ baking method for degassing of a kicker magnet in accelerator beam line
International Nuclear Information System (INIS)
Kamiya, Junichiro; Ogiwara, Norio; Yanagibashi, Toru; Kinsho, Michikazu; Yasuda, Yuichi
2016-01-01
In this study, the authors propose a new in situ degassing method by which only kicker magnets in the accelerator beam line are baked out without raising the temperature of the vacuum chamber to prevent unwanted thermal expansion of the chamber. By simply installing the heater and thermal radiation shield plates between the kicker magnet and the chamber wall, most of the heat flux from the heater directs toward the kicker magnet. The result of the verification test showed that each part of the kicker magnet was heated to above the target temperature with a small rise in the vacuum chamber temperature. A graphite heater was selected in this application to bake-out the kicker magnet in the beam line to ensure reliability and easy maintainability of the heater. The vacuum characteristics of graphite were suitable for heater operation in the beam line. A preliminary heat-up test conducted in the accelerator beam line also showed that each part of the kicker magnet was successfully heated and that thermal expansion of the chamber was negligibly small
In situ baking method for degassing of a kicker magnet in accelerator beam line
Energy Technology Data Exchange (ETDEWEB)
Kamiya, Junichiro, E-mail: kamiya.junichiro@jaea.go.jp; Ogiwara, Norio; Yanagibashi, Toru; Kinsho, Michikazu [Japan Atomic Energy Agency, J-PARC Center, Ooaza Shirakata 2-4, Tokai, Naka, Ibaraki 319-1195 (Japan); Yasuda, Yuichi [SAKAGUCHI E.H VOC CORP., Sakura Dai-san Kogyodanchi 1-8-6, Osaku, Sakura, Chiba 285-0802 (Japan)
2016-03-15
In this study, the authors propose a new in situ degassing method by which only kicker magnets in the accelerator beam line are baked out without raising the temperature of the vacuum chamber to prevent unwanted thermal expansion of the chamber. By simply installing the heater and thermal radiation shield plates between the kicker magnet and the chamber wall, most of the heat flux from the heater directs toward the kicker magnet. The result of the verification test showed that each part of the kicker magnet was heated to above the target temperature with a small rise in the vacuum chamber temperature. A graphite heater was selected in this application to bake-out the kicker magnet in the beam line to ensure reliability and easy maintainability of the heater. The vacuum characteristics of graphite were suitable for heater operation in the beam line. A preliminary heat-up test conducted in the accelerator beam line also showed that each part of the kicker magnet was successfully heated and that thermal expansion of the chamber was negligibly small.
Scanziani, Alessio; Singh, Kamaljit; Blunt, Martin J; Guadagnini, Alberto
2017-06-15
Multiphase flow in porous media is strongly influenced by the wettability of the system, which affects the arrangement of the interfaces of different phases residing in the pores. We present a method for estimating the effective contact angle, which quantifies the wettability and controls the local capillary pressure within the complex pore space of natural rock samples, based on the physical constraint of constant curvature of the interface between two fluids. This algorithm is able to extract a large number of measurements from a single rock core, resulting in a characteristic distribution of effective in situ contact angle for the system, that is modelled as a truncated Gaussian probability density distribution. The method is first validated on synthetic images, where the exact angle is known analytically; then the results obtained from measurements within the pore space of rock samples imaged at a resolution of a few microns are compared to direct manual assessment. Finally the method is applied to X-ray micro computed tomography (micro-CT) scans of two Ketton cores after waterflooding, that display water-wet and mixed-wet behaviour. The resulting distribution of in situ contact angles is characterized in terms of a mixture of truncated Gaussian densities. Crown Copyright © 2017. Published by Elsevier Inc. All rights reserved.
Noise canceling in-situ detection
Walsh, David O.
2014-08-26
Technologies applicable to noise canceling in-situ NMR detection and imaging are disclosed. An example noise canceling in-situ NMR detection apparatus may comprise one or more of a static magnetic field generator, an alternating magnetic field generator, an in-situ NMR detection device, an auxiliary noise detection device, and a computer.
International Nuclear Information System (INIS)
Chen, Jian; Zou, Yong-Cun; Zhang, Feng; Zhang, Yuan-Chun; Guo, Fei-Fan; Li, Guo-Dong
2013-01-01
Highlights: ► LiFePO 4 /C composite was prepared by an in situ polymerization restriction method. ► The size of LiFePO 4 in the composite is effectively restricted. ► The high-rate capability and cycling performance of LiFePO 4 are enhanced greatly. -- Abstract: The LiFePO 4 /C composite is prepared by heating the mixture of resorcinol–formaldehyde gel and FePO 4 , synthesized by an in situ polymerization restriction method, and lithium acetate dihydrate in the atmosphere of nitrogen. The physical and electrochemical properties of the LiFePO 4 /C composite are investigated by X-ray diffraction, Raman spectroscopy, thermogravimetric analysis, scanning electron microscopy, transmission electron microscopy and electrochemical measurements. The discharge capacity of LiFePO 4 is as high as 155.6 mA h g −1 in the first cycle at 0.5C, and it could remain 144.0 mA h g −1 after 50 cycles. Even at the high rates of 10C, 20C and 50C, the initial discharge capacities of the electrodes exhibit 115.6 mA h g −1 , 84.5 mA h g −1 and 67.8 mA h g −1 , and the electrodes deliver capacity retention of 89.5%, 90.9% and 85.7% after 1000 cycles, respectively. The outstanding electrochemical performance could be attributed to the small particle size and good electronic conductivity of the composite
Aquifer restoration at uranium in situ leach sites
International Nuclear Information System (INIS)
Anastasi, F.S.; Williams, R.E.
1985-01-01
In situ mining of uranium involves injection of a leaching solution (lixiviant) into an ore-bearing aquifer. Frequently, the ground water in the mined aquifer is a domestic or livestock water supply. As the lixiviant migrates through the ore body, uranium and various associated elements such as arsenic, selenium, molybdenum, vanadium and radium-226 are mobilized in the ground water. Aquifer restoration after in situ mining is not fully understood. Several methods have been developed to restore mined aquifers to pre-mining (baseline) quality. Commonly used methods include ground water sweeping, clean water injection, and treatment by ion exchange and reverse osmosis technologies. Ammonium carbonate lixiviant was used at one RandD in situ mine. Attempts were made to restore the aquifer using a variety of methods. Efforts were successful in reducing concentrations of the majority of contaminants to baseline levels. Concentrations of certain parameters, however, remained at levels above baseline six months after restoration ceased. Relatively large quantities of ground water were processed in the restoration attempt considering the small size of the project (1.25 acre). More thorough characterization of the hydrogeology of the site may have enhanced the effectiveness of restoration and reduced potential environmental impacts associated with the project. This paper presents some of the findings of a research project conducted by the Mineral Resources Waste Management Team at the University of Idaho in Moscow, Idaho. Views contained herein do not reflect U.S. Nuclear Regulatory Commission policy
Detailed GEANT description of the SDC central calorimeters
International Nuclear Information System (INIS)
Glagolev, V.V.; Li, W.
1994-01-01
This article represents the very detailed simulation model of the SDC central calorimeters and some results which were obtained using that model. The central calorimeters structure was coded on the GEANT 3.15 base in the frame of the SDCSIM environment. The SDCSIM is the general shell for simulation of the SDC set-up. The calorimeters geometry has been coded according to the FNAL and ANL engineering drawings and engineering data file. SDC central calorimeters detailed description is extremely useful for different simulation tasks, for fast simulation program parameters tuning, for different geometry especially studying (local response nonuniformity from bulkheads in the e.m. calorimeter and from coil supports and many others) and for the interpretation of the experimental data from the calorimeters. This simulation model is very useful for tasks of the test beam modules calorimeter calibration and for calorimeter in situ calibration. 3 refs., 8 figs
Energy Technology Data Exchange (ETDEWEB)
Jardine, P.M.; Watson, D.B.; Blake, D.A.; Beard, L.P.; Brooks, S.C.; Carley, J.M.; Criddle, C.S.; Doll, W.E.; Fields, M.W.; Fendorf, S.E.; Geesey, G.G.; Ginder-Vogel, M.; Hubbard, S.S.; Istok, J.D.; Kelly, S.; Kemner, K.M.; Peacock, A.D.; Spalding, B.P.; White, D.C.; Wolf, A.; Wu, W.; Zhou, J.
2004-11-14
Department of Energy (DOE) facilities within the weapons complex face a daunting challenge of remediating huge below inventories of legacy radioactive and toxic metal waste. More often than not, the scope of the problem is massive, particularly in the high recharge, humid regions east of the Mississippi river, where the off-site migration of contaminants continues to plague soil water, groundwater, and surface water sources. As of 2002, contaminated sites are closing rapidly and many remediation strategies have chosen to leave contaminants in-place. In situ barriers, surface caps, and bioremediation are often the remedial strategies of chose. By choosing to leave contaminants in-place, we must accept the fact that the contaminants will continue to interact with subsurface and surface media. Contaminant interactions with the geosphere are complex and investigating long term changes and interactive processes is imperative to verifying risks. We must be able to understand the consequences of our action or inaction. The focus of this manuscript is to describe recent technical developments for assessing the performance of in situ bioremediation and immobilization of subsurface metals and radionuclides. Research within DOE's NABIR and EMSP programs has been investigating the possibility of using subsurface microorganisms to convert redox sensitive toxic metals and radionuclides (e.g. Cr, U, Tc, Co) into a less soluble, less mobile forms. Much of the research is motivated by the likelihood that subsurface metal-reducing bacteria can be stimulated to effectively alter the redox state of metals and radionuclides so that they are immobilized in situ for long time periods. The approach is difficult, however, since subsurface media and waste constituents are complex with competing electron acceptors and hydrogeological conditions making biostimulation a challenge. Performance assessment of in situ biostimulation strategies is also difficult and typically requires detailed
Location specific in situ TEM straining specimens made using FIB
International Nuclear Information System (INIS)
Field, R.D.; Papin, P.A.
2004-01-01
A method has been devised and demonstrated for producing in situ straining specimens for the transmission electron microscope (TEM) from specific locations in a sample using a dual-beam focused ion beam (FIB) instrument. The specimen is removed from a polished surface in the FIB using normal methods and then attached to a pre-fabricated substrate in the form of a modified TEM tensile specimen. In this manner, specific features of the microstructure of a polished optical mount can be selected for in situ tensile straining. With the use of electron backscattered diffraction (EBSD), this technique could be extended to select specific orientations of the specimen as well
Energy Technology Data Exchange (ETDEWEB)
Hong, Sang Bum; Nam, Jong Soo; Choi, Yong Suk; Seo, Bum Kyoung; Moon, Jei Kwon [Korea Atomic Energy Research Institute, Daejeon (Korea, Republic of)
2016-06-15
In situ gamma spectrometry has been used to measure environmental radiation, assumptions are usually made about the depth distribution of the radionuclides of interest in the soil. The main limitation of in situ gamma spectrometry lies in determining the depth distribution of radionuclides. The objective of this study is to develop a method for subsurface characterization by in situ measurement. The peak to valley method based on the ratio of counting rate between the photoelectric peak and Compton region was applied to identify the depth distribution. The peak to valley method could be applied to establish the relation between the spectrally derived coefficients (Q) with relaxation mass per unit area (β) for various depth distribution in soil. The in situ measurement results were verified by MCNP simulation and calculated correlation equation. In order to compare the depth distributions and contamination levels in decommissioning KRR site, in situ measurement and sampling results were compared. The in situ measurement results and MCNP simulation results show a good correlation for laboratory measurement. The simulation relationship between Q and source burial for the source layers have exponential relationship for a variety depth distributions. We applied the peak to valley method to contaminated decommissioning KRR site to determine a depth distribution and initial activity without sampling. The observed results has a good correlation, relative error between in situ measurement with sampling result is around 7% for depth distribution and 4% for initial activity. In this study, the vertical activity distribution and initial activity of {sup 137}Cs could be identifying directly through in situ measurement. Therefore, the peak to valley method demonstrated good potential for assessment of the residual radioactivity for site remediation in decommissioning and contaminated site.
Energy Technology Data Exchange (ETDEWEB)
Wang Xingyan, E-mail: wxianyou@yahoo.com [Key Laboratory of Environmentally Friendly Chemistry and Applications of Ministry of Education, Chemistry School, Xiangtan University, Hunan, Xiangtan 411105 (China); Hunan Institute of Humanities Science and Technology, Loudi 417000 (China); Key Laboratory of Materials Design and Preparation Technology of Hunan, Xiangtan 411105 (China); Liu Li [Key Laboratory of Environmentally Friendly Chemistry and Applications of Ministry of Education, Chemistry School, Xiangtan University, Hunan, Xiangtan 411105 (China); Wang Xianyou, E-mail: wqinyan801@yahoo.com.cn [Key Laboratory of Environmentally Friendly Chemistry and Applications of Ministry of Education, Chemistry School, Xiangtan University, Hunan, Xiangtan 411105 (China); Key Laboratory of Materials Design and Preparation Technology of Hunan, Xiangtan 411105 (China); Yi Lanhua [Key Laboratory of Environmentally Friendly Chemistry and Applications of Ministry of Education, Chemistry School, Xiangtan University, Hunan, Xiangtan 411105 (China); Hu Chuanyue [Hunan Institute of Humanities Science and Technology, Loudi 417000 (China); Zhang Xiaoyan [Key Laboratory of Environmentally Friendly Chemistry and Applications of Ministry of Education, Chemistry School, Xiangtan University, Hunan, Xiangtan 411105 (China)
2011-09-15
Highlights: > Mn{sub 2}O{sub 3}/CAMB composite materials for supercapacitor were prepared by in situ coating method. > The optimum amount of Mn{sub 2}O{sub 3} in Mn{sub 2}O{sub 3}/CAMB composite is 10 wt%. > Coating nano-sized Mn{sub 2}O{sub 3} on the CAMB could improve the supercapacitive behaviors of composites. - Abstract: A series of Mn{sub 2}O{sub 3}/carbon aerogel microbead (Mn{sub 2}O{sub 3}/CAMB) composites for supercapacitor electrodes have been synthesized by in situ encapsulation method. The structure and morphology of Mn{sub 2}O{sub 3}/CAMB are characterized by X-ray diffraction (XRD), Fourier transform infrared (FT-IR) spectrum and scanning electron microscopy (SEM). Electrochemical performances of the synthesized composites are evaluated by cyclic voltammetry and galvanostatic charge/discharge measurement. All the composites with different Mn{sub 2}O{sub 3} contents show higher specific capacitance than pure CAMB due to the pseudo-capacitance of the Mn{sub 2}O{sub 3} particles dispersed on the surface of CAMB. The highest specific capacitance is up to 368.01 F g{sup -1} when 10 wt% Mn{sub 2}O{sub 3} is coated on the surface of CAMB. Besides, 10%-Mn{sub 2}O{sub 3}/CAMB supercapacitor exhibits excellent cyclic stability, the specific capacitance still retains 90% of initial capacitance over 5000 cycles.
Jonge, de L.H.; Laar, van H.; Hendriks, W.H.; Dijkstra, J.
2013-01-01
A modified rinsing method for the in situ technique was developed to separate, isolate and characterise the soluble (S), the insoluble washout (W–S) and the non-washout fractions (D1U) within one procedure. For non-incubated bags ( t50 h), this method was compared with the conventional, Combined
Directory of Open Access Journals (Sweden)
Xingkai Zhang
2017-06-01
Full Text Available A simple and feasible method was developed to fabricate in-situ reduced graphene oxide-polyvinyl alcohol composite (GO-PVA coatings as protective layers on magnesium substrates. Polyvinyl alcohol was used as an in-situ reductant to transform GO into reduced GO. Contiguous and uniform GO-PVA coatings were prepared on magnesium substrates by dip-coating method, and were further thermally treated at 120 °C under ambient condition to obtain in-situ reduced GO-PVA coatings. Owing to the reducing effect of PVA, thermal treatment at low temperature led to effective in-situ reduction of GO as confirmed by XRD, Raman, FTIR and XPS tests. The corrosion current density of magnesium substrates in 3.5 wt% NaCl solution could be lowered to its 1/25 when using in-situ reduced GO-PVA coatings as protective layers.
Energy Technology Data Exchange (ETDEWEB)
Nishikata, U. [Kansai Electric Power Co. Inc., Osaka (Japan)
1998-12-10
Development was made on a simplified site-use single face shear testing device and a test method that can perform in-situ and direct measurement during construction on shear strength of coarse particle materials having large diameters. The test device consists of shear frames embedded in the in-situ ground bed, a weight for vertical loading, and a traction device to load horizontal force. In the test, prescribed rolling compaction is performed by a vibration roller which presses in the shear frames into unwound banking by using a four-piece vibration roller. Crushed stones are piled on cells of the shear frames to the same height. The specified weight is placed thereon via a loading plate, which is used as the vertical load. The shear force is loaded by horizontally pulling chains connected to the shear frames by using the traction device, and is measured by a load cell. The vertical displacement is measured by two vertical displacement meters, and the horizontal displacement by one horizontal displacement meter. A verification test is continuing using the test device. Four cases with different vertical stresses can be tested in about one day. (NEDO)
International Nuclear Information System (INIS)
Frenzel, E.
2002-01-01
The compact and mobile Triathler liquid scintillation counter enables in situ measurements of 222 Rn in water ( LLoD 226 Ra ( enrichment by filtering on special 3M RadDisks); (iv) 228 Ra (enrichment by filtering on special 3M RadDisks ). The special rapid method for radium 226 and 228 benefits from the availability of selective filters of 3M company. The filters were developed by US research centres ( like the Argonne National Laboratories) in collaboration with 3M. Up to 5 liters are filtered through the RadDisks and subsequently measured preferable by LSC, or Low Background Counting or α spectroscopy
A detailed survey of numerical methods for unconstrained minimization. Pt. 1
International Nuclear Information System (INIS)
Mika, K.; Chaves, T.
1980-01-01
A detailed description of numerical methods for unconstrained minimization is presented. This first part surveys in particular conjugate direction and gradient methods, whereas variable metric methods will be the subject of the second part. Among the results of special interest we quote the following. The conjugate direction methods of Powell, Zangwill and Sutti can be best interpreted if the Smith approach is adopted. The conditions for quadratic termination of Powell's first procedure are analyzed. Numerical results based on nonlinear least squares problems are presented for the following conjugate direction codes: VA04AD from Harwell Subroutine Library and ZXPOW from IMSL, both implementations of Powell's second procedure, DFMND from IBM-SILMATH (Zangwill's method) and Brent's algorithm PRAXIS. VA04AD turns out to be superior in all cases, PRAXIS improves for high-dimensional problems. All codes clearly exhibit superlinear convergence. Akaike's result for the method of steepest descent is derived directly from a set of nonlinear recurrence relations. Numerical results obtained with the highly ill conditioned Hilbert function confirm the theoretical predictions. Several properties of the conjugate gradient method are presented and a new derivation of the equivalence of steepest descent partan and the CG method is given. A comparison of numerical results from the CG codes VA08AD (Fletcher-Reeves), DFMCG (the SSP version of the Fletcher-Reevens algorithm) and VA14AD (Powell's implementation of the Polak-Ribiere formula) reveals that VA14AD is clearly superior in all cases, but that the convergence rate of these codes is only weakly superlinear such that high accuracy solutions require extremely large numbers of function calls. (orig.)
In situ calibration of acoustic emission transducers by time reversal method
Czech Academy of Sciences Publication Activity Database
Kober, Jan; Převorovský, Zdeněk; Chlada, Milan
2016-01-01
Roč. 240, April (2016), s. 50-56 ISSN 0924-4247 Institutional support: RVO:61388998 Keywords : time reversed acoustic s * calibration * in situ * acoustic emission Subject RIV: BI - Acoustic s Impact factor: 2.499, year: 2016 http://ac.els-cdn.com/S0924424716300334/1-s2.0-S0924424716300334-main.pdf?_tid=0acf4736-ef6d-11e5-b826-00000aacb362&acdnat=1458568911_1c21eda9762b905a684ff939463ef3fe
In-situ Planetary Subsurface Imaging System
Song, W.; Weber, R. C.; Dimech, J. L.; Kedar, S.; Neal, C. R.; Siegler, M.
2017-12-01
Geophysical and seismic instruments are considered the most effective tools for studying the detailed global structures of planetary interiors. A planet's interior bears the geochemical markers of its evolutionary history, as well as its present state of activity, which has direct implications to habitability. On Earth, subsurface imaging often involves massive data collection from hundreds to thousands of geophysical sensors (seismic, acoustic, etc) followed by transfer by hard links or wirelessly to a central location for post processing and computing, which will not be possible in planetary environments due to imposed mission constraints on mass, power, and bandwidth. Emerging opportunities for geophysical exploration of the solar system from Venus to the icy Ocean Worlds of Jupiter and Saturn dictate that subsurface imaging of the deep interior will require substantial data reduction and processing in-situ. The Real-time In-situ Subsurface Imaging (RISI) technology is a mesh network that senses and processes geophysical signals. Instead of data collection then post processing, the mesh network performs the distributed data processing and computing in-situ, and generates an evolving 3D subsurface image in real-time that can be transmitted under bandwidth and resource constraints. Seismic imaging algorithms (including traveltime tomography, ambient noise imaging, and microseismic imaging) have been successfully developed and validated using both synthetic and real-world terrestrial seismic data sets. The prototype hardware system has been implemented and can be extended as a general field instrumentation platform tailored specifically for a wide variety of planetary uses, including crustal mapping, ice and ocean structure, and geothermal systems. The team is applying the RISI technology to real off-world seismic datasets. For example, the Lunar Seismic Profiling Experiment (LSPE) deployed during the Apollo 17 Moon mission consisted of four geophone instruments
In situ vitrification laboratory-scale test work plan
International Nuclear Information System (INIS)
Nagata, P.K.; Smith, N.L.
1991-05-01
The Buried Waste Program was established in October 1987 to accelerate the studies needed to develop a long-term management plan for the buried mixed waste at the Radioactive Waste Management Complex at Idaho Engineering Laboratory. The In Situ Vitrification Project is being conducted in a Comprehensive Environmental Response, Compensation, and Liability Act feasibility study format to identify methods for the long-term management of mixed buried waste. To support the overall feasibility study, the situ vitrification treatability investigations are proceeding along the three parallel paths: laboratory-scale tests, intermediate field tests, and field tests. Laboratory-scale tests are being performed to provide data to mathematical modeling efforts, which, in turn, will support design of the field tests and to the health and safety risk assessment. This laboratory-scale test work plan provides overall testing program direction to meet the current goals and objectives of the in situ vitrification treatability investigation. 12 refs., 1 fig., 7 tabs
In-Situ Determination Of Actual Evapotranspiration Using Zero Flux ...
African Journals Online (AJOL)
This paper presents a method of determining in-situ actual evapotranspiration using water balance method in the case of maize crop grown for two seasons at the Experimental Farm of the Department of Agricultural Engineering, Federal University of Technology, Akure in a non-saturated soil condition. The crop was ...
Cell In Situ Zymography: Imaging Enzyme-Substrate Interactions.
Chhabra, Aastha; Rani, Vibha
2017-01-01
Zymography has long been used for the detection of substrate-specific enzyme activity. In situ zymography (ISZ), an adaptation from the conventional substrate zymography, is a widely employed technique useful for the detection, localization, and estimation of enzyme-substrate interactions in tissues. Here, we describe a protocol to detect 'in position' matrix metalloproteinase (MMP) activity in cells utilizing H9c2 cardiomyoblasts as a model. This technique is primarily adopted from the method used for histological sections and is termed as 'Cell in situ Zymography'. It is a simple, sensitive, and quantifiable methodology to assess the functional activity of an enzyme 'on site/in position' in cell culture.
In situ imaging and proteome profiling indicate andrographolide is a highly promiscuous compound
Li, Lin; Wijaya, Hadhi; Samanta, Sanjay; Lam, Yulin; Yao, Shao Q.
2015-06-01
Natural products represent an enormous source of pharmacologically useful compounds, and are often used as the starting point in modern drug discovery. Many biologically interesting natural products are however not being pursued as potential drug candidates, partly due to a lack of well-defined mechanism-of-action. Traditional in vitro methods for target identification of natural products based on affinity protein enrichment from crude cellular lysates cannot faithfully recapitulate protein-drug interactions in living cells. Reported herein are dual-purpose probes inspired by the natural product andrographolide, capable of both reaction-based, real-time bioimaging and in situ proteome profiling/target identification in live mammalian cells. Our results confirm that andrographolide is a highly promiscuous compound and engaged in covalent interactions with numerous previously unknown cellular targets in cell type-specific manner. We caution its potential therapeutic effects should be further investigated in detail.
An introduction to in-situ produced cosmogenic nuclides
International Nuclear Information System (INIS)
Norton, K.P.
2012-01-01
Cosmogenic nuclides are produced through interactions between cosmic rays and target nuclei in Earth's atmosphere and surface materials. Those which are produced in Earth's atmosphere are termed 'meteoric' while the nuclides produced in surface material are known as in-situ cosmogenic nuclides. The past two decades have seen a proliferation of applications for cosmogenic nuclides. This is primarily due to a revolution in accelerator mass spectrometry, AMS, measurement techniques which has allowed the measurement of very small amounts of nuclides. The following is a brief introduction to the theory and application of in-situ produced cosmogenic nuclide methods. (author). 17 refs., figs., 1 tab.
Pedersen, Marianne; Rasmussen, Birgitte Bruun
2009-06-01
Fluorescence in situ hybridization (FISH) is regarded as the gold standard method for detecting HER2 gene amplification. Chromogenic in situ hybridization (CISH) is a promising alternative to FISH because CISH has the advantages of being a method evaluated by bright-field microscopy and the generated chromogenic signals are also stable. This study presents a dual color CISH for simultaneous detection of the HER2 gene and chromosome 17. The CISH method performs a chromogenic detection "on top" of the Food and Drug Administration (FDA)-approved HER2 FISH pharmDx method, where the fluorochrome-labeled probes are detected using enzyme-labeled antibodies and visualized by chromogenic enzymatic reactions. The HER2 status (amplified/not amplified and HER2 ratios) was evaluated by the CISH method and compared with results obtained by the FDA-approved FISH method. Of the 72 successfully investigated invasive breast carcinomas, both FISH and CISH detected HER2 amplification in 24 cases and nonamplification was detected in 47 cases. One case showed a discrepancy between FISH and CISH. The concordance between CISH and FISH was found to be almost perfect (98.6%). The correlation between the HER2 ratios obtained by the 2 methods showed excellent correlation (correlation coefficient 0.95). In conclusion, it is possible by dual-color CISH method to demonstrate HER2 genes and chromosome 17 genes, in the same tissue section and reliably assess HER2 status. The CISH method is a very promising alternative to the FISH method.
Energy Technology Data Exchange (ETDEWEB)
Liu, Xiang, E-mail: liuxiang@ahut.edu.cn; Zhao, Tiantian; Liu, Pengpeng; Cui, Ping, E-mail: cokecp@sohu.com; Hu, Peng
2015-03-01
A “bottom up” approach of manufacturing graphite oxides (GOs) derived from aqueous glucose solutions by virtue of an environmentally-friendly process and the way of in-situ synthesizing magnetite–GOs composites are described in this work in detail. The dehydrations among glucose molecules under hydrothermal condition result in the initial carbon quantum dots and ultimate GOs. The structural information of the GOs is obtained by the infrared, ultraviolet–visible and X-ray photoelectron spectra. The magnetite–GOs composites were obtained by a one-pot method under the same hydrothermal conditions as the one of preparing GOs. The composites perform high activities in catalytic degradation of Rhodamine B in the presence of hydrogen peroxides without extra heating or pH adjusting. Both the GOs and the magnetite–GOs composites are also assured by measurements of transmission electron microscope and X-ray powder diffraction. - Highlights: • Graphite oxides are made from aqueous glucose solutions by hydrothermal reaction. • A way of in-situ synthesizing composites of magnetite–graphite oxides is depicted. • The composites perform high activities in catalytic degradation of Rhodamine B.
Life Support Catalyst Regeneration Using Ionic Liquids and In Situ Resources
Abney, Morgan B.; Karr, Laurel; Paley, Mark S.; Donovan, David N.
2016-01-01
Oxygen recovery from metabolic carbon dioxide is an enabling capability for long-duration manned space flight. Complete recovery of oxygen (100%) involves the production of solid carbon. Catalytic approaches for this purpose, such as Bosch technology, have been limited in trade analyses due in part to the mass penalty for high catalyst resupply caused by carbon fouling of the iron or nickel catalyst. In an effort to mitigate this challenge, several technology approaches have been proposed. These approaches have included methods to prolong the life of the catalysts by increasing the total carbon mass loading per mass catalyst, methods for simplified catalyst introduction and removal to limit the resupply container mass, methods of using in situ resources, and methods to regenerate catalyst material. Research and development into these methods is ongoing, but only use of in situ resources and/or complete regeneration of catalyst material has the potential to entirely eliminate the need for resupply. The use of ionic liquids provides an opportunity to combine these methods in a technology approach designed to eliminate the need for resupply of oxygen recovery catalyst. Here we describe the results of an initial feasibility study using ionic liquids and in situ resources for life support catalyst regeneration, we discuss the key challenges with the approach, and we propose future efforts to advance the technology.
In situ Measurements of Phytoplankton Fluorescence Using Low Cost Electronics
Directory of Open Access Journals (Sweden)
Dana L. Wright
2013-06-01
Full Text Available Chlorophyll a fluorometry has long been used as a method to study phytoplankton in the ocean. In situ fluorometry is used frequently in oceanography to provide depth-resolved estimates of phytoplankton biomass. However, the high price of commercially manufactured in situ fluorometers has made them unavailable to some individuals and institutions. Presented here is an investigation into building an in situ fluorometer using low cost electronics. The goal was to construct an easily reproducible in situ fluorometer from simple and widely available electronic components. The simplicity and modest cost of the sensor makes it valuable to students and professionals alike. Open source sharing of architecture and software will allow students to reconstruct and customize the sensor on a small budget. Research applications that require numerous in situ fluorometers or expendable fluorometers can also benefit from this study. The sensor costs US$150.00 and can be constructed with little to no previous experience. The sensor uses a blue LED to excite chlorophyll a and measures fluorescence using a silicon photodiode. The sensor is controlled by an Arduino microcontroller that also serves as a data logger.
Koichumanova, Kamila; Visan, Aura; Geerdink, Bert; Lammertink, Rob G.H.; Mojet, Barbara; Seshan, Kulathuiyer; Lefferts, Leonardus
2017-01-01
An in situ ATR-IR spectroscopic cell suitable for studies at solid-liquid interface is described including the design and experimental details in continuous flow mode at elevated temperatures (230 °C) and pressures (30 bar). The design parameters considered include the cell geometry, the procedure
In situ polymerization of monomers for polyphenylquinoxaline/graphite
Serafini, T. T.; Delvigs, P.; Vannucci, R. D.
1973-01-01
Methods currently used to prepare fiber reinforced, high temperature resistant polyphenylquinoxaline (PPQ) composites employ extremely viscous, low solids content solutions of high molecular weight PPQ polymers. An improved approach, described in this report, consists of impregnating the fiber with a solution of the appropriate monomers instead of a solution of previously synthesized high molecular weight polymer. Polymerization of the monomers occurs in situ on the fiber during the solvent removal and curing stages. The in situ polymerization approach greatly simplifies the fabrication of PPQ graphite fiber composites. The use of low viscosity monomeric type solutions facilitates fiber wetting, permits a high solids content, and eliminates the need for prior polymer synthesis.
Directory of Open Access Journals (Sweden)
Arzu Erol Tunç
2017-10-01
Full Text Available The objectives of this study were to estimate the protein degradability of extruded full fat soybean (ESB by in situ (nylon bag and in vitro enzymatic method and to develop an equation in order predict in situ degradability from in vitro values. In the study enzymatic technique; hydrolysis after 1 h (INV1 and after 24 h (INV24 by a purified protease extracted from Streptomyces griseus in a borate-phosphate buffer at pH 8 was used as in vitro method. Relationship between in situ effective protein degradability (INSE and in vitro degradability after 1 and 24 hours incubations (INV1 and INV24 were determined. In situ protein degradability was measured at 0, 2, 4, 8, 16, 24, and 48 and at 72 h incubations in the rumen of 3 Holstein cows. In the study INSE, INV1 and INV24 were determined as 58.05, 20.24 and 41.46% respectively. Despite there were differences between in situ and in vitro protein degradability values, correlation coefficients between in situ and in vitro protein degradability of ESB were high and regression equations for estimation of in situ from in vitro were found significant. As conclusion in vitro enzymatic protein degradability (INV1 and INV24 can be used for estimation of in situ effective protein degradability of extruded full fat soybean.
In-Situ Testing of the Thermal Diffusivity of Polysilicon Thin Films
Directory of Open Access Journals (Sweden)
Yi-Fan Gu
2016-10-01
Full Text Available This paper presents an intuitive yet effective in-situ thermal diffusivity testing structure and testing method. The structure consists of two doubly clamped beams with the same width and thickness but different lengths. When the electric current is applied through two terminals of one beam, the beam serves as thermal resistor and the resistance R(t varies as temperature rises. A delicate thermodynamic model considering thermal convection, thermal radiation, and film-to-substrate heat conduction was established for the testing structure. The presented in-situ thermal diffusivity testing structure can be fabricated by various commonly used micro electro mechanical systems (MEMS fabrication methods, i.e., it requires no extra customized processes yet provides electrical input and output interfaces for in-situ testing. Meanwhile, the testing environment and equipment had no stringent restriction, measurements were carried out at normal temperatures and pressures, and the results are relatively accurate.
In situ measurements reveal extremely low pH in soil
DEFF Research Database (Denmark)
Nielsen, Knud Erik; Loibide, Amaia Irixar; Nielsen, Lars Peter
2017-01-01
We measured pH in situ in the top organic soil horizons in heathland and pine forest and found values between 2.6 and 3.2. This was 0.5e0.8 units lower than concurrent laboratory pH measurements of the same soil, which raises questions about the interpretation of pH measurements. We propose that ...... that the higher pH recorded by standard laboratory methods may be due to buffering ions from soil biota released from drying, grinding and rewetting of soil samples, whereas the in situ pH reflects the correct level of acidification....
Data on a Laves phase intermetallic matrix composite in situ toughened by ductile precipitates.
Knowles, Alexander J; Bhowmik, Ayan; Purkayastha, Surajit; Jones, Nicholas G; Giuliani, Finn; Clegg, William J; Dye, David; Stone, Howard J
2017-10-01
The data presented in this article are related to the research article entitled "Laves phase intermetallic matrix composite in situ toughened by ductile precipitates" (Knowles et al.) [1]. The composite comprised a Fe 2 (Mo, Ti) matrix with bcc (Mo, Ti) precipitated laths produced in situ by an aging heat treatment, which was shown to confer a toughening effect (Knowles et al.) [1]. Here, details are given on a focused ion beam (FIB) slice and view experiment performed on the composite so as to determine that the 3D morphology of the bcc (Mo, Ti) precipitates were laths rather than needles. Scanning transmission electron microscopy (S(TEM)) micrographs of the microstructure as well as energy dispersive X-ray spectroscopy (EDX) maps are presented that identify the elemental partitioning between the C14 Laves matrix and the bcc laths, with Mo rejected from the matrix into laths. A TEM selected area diffraction pattern (SADP) and key is provided that was used to validate the orientation relation between the matrix and laths identified in (Knowles et al.) [1] along with details of the transformation matrix determined.
Data on a Laves phase intermetallic matrix composite in situ toughened by ductile precipitates
Directory of Open Access Journals (Sweden)
Alexander J. Knowles
2017-10-01
Full Text Available The data presented in this article are related to the research article entitled “Laves phase intermetallic matrix composite in situ toughened by ductile precipitates” (Knowles et al. [1]. The composite comprised a Fe2(Mo, Ti matrix with bcc (Mo, Ti precipitated laths produced in situ by an aging heat treatment, which was shown to confer a toughening effect (Knowles et al. [1]. Here, details are given on a focused ion beam (FIB slice and view experiment performed on the composite so as to determine that the 3D morphology of the bcc (Mo, Ti precipitates were laths rather than needles. Scanning transmission electron microscopy (S(TEM micrographs of the microstructure as well as energy dispersive X-ray spectroscopy (EDX maps are presented that identify the elemental partitioning between the C14 Laves matrix and the bcc laths, with Mo rejected from the matrix into laths. A TEM selected area diffraction pattern (SADP and key is provided that was used to validate the orientation relation between the matrix and laths identified in (Knowles et al. [1] along with details of the transformation matrix determined.
Alam, Md. Fazle; Laskar, Amaj Ahmed; Ahmed, Shahbaz; Shaida, Mohd. Azfar; Younus, Hina
2017-08-01
Melamine toxicity has recently attracted worldwide attention as it causes renal failure and the death of humans and animals. Therefore, developing a simple, fast and sensitive method for the routine detection of melamine is the need of the hour. Herein, we have developed a selective colorimetric method for the detection of melamine in milk samples based upon in-situ formation of silver nanoparticles (AgNPs) via tannic acid. The AgNPs thus formed were characterized by UV-Visible spectrophotometer, transmission electron microscope (TEM), zetasizer and dynamic light scattering (DLS). The AgNPs were used to detect melamine under in vitro condition and in raw milk spiked with melamine. Under optimal conditions, melamine could be selectively detected in vitro within the concentration range of 0.05-1.4 μM with a limit of detection (LOD) of 0.01 μM, which is lower than the strictest melamine safety requirement of 1 ppm. In spiked raw milk, the recovery percentage range was 99.5-106.5% for liquid milk and 98.5-105.5% for powdered milk. The present method shows extreme selectivity with no significant interference with other substances like urea, glucose, glycine, ascorbic acid etc. This assay method does not utilize organic cosolvents, enzymatic reactions, light sensitive dye molecules and sophisticated instrumentation, thereby overcoming some of the limitations of the other conventional methods.
Francis, Glenn D; Jones, Mark A; Beadle, Geoffrey F; Stein, Sandra R
2009-06-01
HER2 gene amplification or overexpression occurs in 15% to 25% of breast cancers and has implications for treatment and prognosis. The most commonly used methods for HER2 testing are fluorescence in situ hybridization (FISH) and immunohistochemistry. FISH is considered to be the reference standard and more accurately predicts response to trastuzumab, but is technically demanding, expensive, and requires specialized equipment. In situ hybridization is required to be eligible for adjuvant treatment with trastuzumab in Australia. Bright-field in situ hybridization is an alternative to FISH and uses a combination of in situ methodology and a peroxidase-mediated chromogenic substrate such as diaminobenzidine [chromogenic in situ hybridization (CISH)] or multimer technology coupled with enzyme metallography [silver-enhanced in situ hybridization (SISH)] to create a marker visible under bright-field microscopy. CISH was introduced into diagnostic testing in Australia in October 2006. SISH methodology is a more recent introduction into the testing repertoire. An evaluation of CISH and SISH performance to assess patient outcome were performed using tissue microarrays. Tissue microarrays were constructed in duplicate using material from 593 patients with invasive breast carcinoma and assessed using CISH and SISH. Gene amplification was assessed using the American Society of Clinical Oncology/College of American Pathologists guideline and Australian HER2 Advisory Board criteria (single probe: diploid, 1 to 2.5 copies/nucleus; polysomy >2.5 to 4 copies/nucleus; equivocal, >4 to 6 copies/nucleus; low-level amplification, >6 to 10 copies/nucleus and high-level amplification >10 copies/nucleus; dual probe HER2/CHR17 ratio: nonamplified 2.2). Results were informative for 337 tissue cores comprising 230 patient samples. Concordance rates were 96% for HER2 single probe CISH and SISH and 95.5% for single probe CISH and dual probe HER2/CHR17 SISH. Both bright-field methods correlated
Groundwater restoration with in situ uranium leach mining
International Nuclear Information System (INIS)
Charbeneau, R.J.
1984-01-01
In situ leach mining of uranium has developed into a major mining technology. Since 1975, when the first commercial mine was licensed in the United States, the percentage or uranium produced by in situ mining has steadily grown from 0.6 to 10 percent in 1980. Part of the reason for this growth is that in situ mining offers less initial capital investment, shorter start-up times, greater safety, and less labor than conventional mining methods. There is little disturbance of the surface terrain or surface waters, no mill tailings piles, and no large open pits, but in situ leaching mining does have environmental disadvantages. During the mining, large amounts of ground water are cirulated and there is some withdrawal from an area where aquifers constitute a major portion of the water supply for other purposes. When an ammonia-based leach system is used, the ammonium ion is introduced into an area where cation exchange on clays (and some production of nitrate) may occur. Also, injection of an oxidant with the leach solution causes valence and phase changes of indigenous elements such as As, Cu, Fe, Mo, Se, S, and V as well as U. Furthermore, the surrounding ground water can become contaminated by escape of the leach solution from the mining zone. This chapter presents an overview of the in situ mining technology, including uranium deposition, mining techniques, and ground water restoration alternatives. The latter part of the chapter covers the situation in South Texas. Economics and development of the industry, groundwater resources, regulation, and restoration activities are also reviewed
International Nuclear Information System (INIS)
Xu Donghua; Wirth, Brian D.; Li Meimei; Kirk, Marquis A.
2012-01-01
We present a combinatorial approach that integrates state-of-the-art transmission electron microscopy (TEM) in situ irradiation experiments and high-performance computing techniques to study irradiation defect dynamics in metals. Here, we have studied the evolution of visible defect clusters in nanometer-thick molybdenum foils under 1 MeV krypton ion irradiation at 80 °C through both cluster dynamics modeling and in situ TEM experiments. The experimental details are reported elsewhere; we focus here on the details of model construction and comparing the model with the experiments. The model incorporates continuous production of point defects and/or small clusters, and the accompanying interactions, which include clustering, recombination and loss to the surfaces that result from the diffusion of the mobile defects. To account for the strong surface effect in thin TEM foils, the model includes one-dimensional spatial dependence along the foil depth, and explicitly treats the surfaces as black sinks. The rich amount of data (cluster number density and size distribution at a variety of foil thickness, irradiation dose and dose rate) offered by the advanced in situ experiments has allowed close comparisons with computer modeling and permitted significant validation and optimization of the model in terms of both physical model construct (damage production mode, identities of mobile defects) and parameterization (diffusivities of mobile defects). The optimized model exhibits good qualitative and quantitative agreement with the in situ TEM experiments. The combinatorial approach is expected to bring a unique opportunity for the study of radiation damage in structural materials.
Sakuraoka, Yuhki; Sawada, Tokihiko; Shiraki, Takayuki; Park, Kyunghwa; Sakurai, Yuhichiro; Tomosugi, Naohisa; Kubota, Keiichi
2012-07-28
To establish methods for quantitative polymerase chain reaction (PCR) for hepcidin using RNAs isolated from paraffin-embedded sections and in situ hybridization of hepatocellular carcinoma (HCC). Total RNA from paraffin-embedded sections was isolated from 68 paraffin-embedded samples of HCC. Samples came from 54 male and 14 female patients with a mean age of 66.8 ± 7.8 years. Quantitative PCR was performed. Immunohistochemistry and in situ hybridization for hepcidin were also performed. Quantitative PCR for hepcidin using RNAs isolated from paraffin-embedded sections of HCC was performed successfully. The expression level of hepcidin mRNA in cancer tissues was significantly higher than that in non-cancer tissues. A method of in situ hybridization for hepcidin was established successfully, and this demonstrated that hepcidin mRNA was expressed in non-cancerous tissue but absent in cancerous tissue. We have established novel methods for quantitative PCR for hepcidin using RNAs isolated from paraffin-embedded sections and in situ hybridization of HCC.
Zhang, Wendong; Dong, Xin'an; Jia, Bin; Zhong, Junbo; Sun, Yanjuan; Dong, Fan
2018-02-01
Novel two-dimensional (2D) BiOCl/Bi12O17Cl2 nanojunctions were fabricated by a facile one-pot in situ method at room temperature. The as-prepared samples were analyzed by XRD, SEM, TEM, HRTEM, UV-vis DRS, PL, ESR and BET-BJH measurement in detail. The photocatalytic performance of the samples was evaluated by removal of NO at ppb level under visible-light illumination. The result reveals that the BiOCl/Bi12O17Cl2 nanojunctions manifests conspicuously enhanced photocatalytic efficiency for NO removal. The facilitated performance can be ascribed to the well-matched band structure and relatively high specific surface area. In addition, the in situ diffuse reflectance infrared Fourier transform spectroscopy was applied to investigate the adsorption and photocatalytic NO oxidation processes. The reaction mechanism of photocatalytic NO oxidation was proposed based on the observed intermediates. The present work could pave a way to synthesize novel visible light photocatalysts at room temperature for environmental application.
Experimental Investigation and High Resolution Simulation of In-Situ Combustion Processes
Energy Technology Data Exchange (ETDEWEB)
Margot Gerritsen; Tony Kovscek
2008-04-30
This final technical report describes work performed for the project 'Experimental Investigation and High Resolution Numerical Simulator of In-Situ Combustion Processes', DE-FC26-03NT15405. In summary, this work improved our understanding of in-situ combustion (ISC) process physics and oil recovery. This understanding was translated into improved conceptual models and a suite of software algorithms that extended predictive capabilities. We pursued experimental, theoretical, and numerical tasks during the performance period. The specific project objectives were (i) identification, experimentally, of chemical additives/injectants that improve combustion performance and delineation of the physics of improved performance, (ii) establishment of a benchmark one-dimensional, experimental data set for verification of in-situ combustion dynamics computed by simulators, (iii) develop improved numerical methods that can be used to describe in-situ combustion more accurately, and (iv) to lay the underpinnings of a highly efficient, 3D, in-situ combustion simulator using adaptive mesh refinement techniques and parallelization. We believe that project goals were met and exceeded as discussed.
Calibration and application of a HPGe gamma spectrometer for in-situ measurements
International Nuclear Information System (INIS)
Xiao Xuefu; Yue Qingyu
1992-02-01
The principle and methods of the calibration for an in-situ γ spectrometer are introduced. The calibration for a portable HPGe γ spectrometer has been completed. The N f /A(peak count rate per unit activity in soil) and N f /D(peak count rate per unit absorbed dose rate in the air) are listed. The uncertainties of the calibration factors are estimated. The in-situ measurements have been carried out in surroundings near the nuclear facilities and the data are compared with those measured by other methods
In-situ trainable intrusion detection system
Energy Technology Data Exchange (ETDEWEB)
Symons, Christopher T.; Beaver, Justin M.; Gillen, Rob; Potok, Thomas E.
2016-11-15
A computer implemented method detects intrusions using a computer by analyzing network traffic. The method includes a semi-supervised learning module connected to a network node. The learning module uses labeled and unlabeled data to train a semi-supervised machine learning sensor. The method records events that include a feature set made up of unauthorized intrusions and benign computer requests. The method identifies at least some of the benign computer requests that occur during the recording of the events while treating the remainder of the data as unlabeled. The method trains the semi-supervised learning module at the network node in-situ, such that the semi-supervised learning modules may identify malicious traffic without relying on specific rules, signatures, or anomaly detection.
In-situ Measurements and Analysis of Naturally Occurring Radioactive Materials
International Nuclear Information System (INIS)
Mueller, W.F.; Ilie, G.; Russ, W.R.; Lange, H.J.; Rotty, M.
2013-06-01
The measurement and quantification of naturally occurring radioactive materials (NORM) is an important element of workplace radioprotection in key industries such as oil and gas production, heavy metals mining and refining, coal burning waste, and water treatment. Monitoring of NORM content in home building materials is another challenge for human safety in the prevention of chronic dose uptake. Materials are classified NORM in case they contain significant amounts of the decay chains of U-238 (Ra-226 as a long lived daughter), U-235 or Th- 232 or the primordial nuclide K-40. Due to the decay of the radionuclides, gamma rays with a signature in the energy range from 45 keV up to 2615 keV are emitted. The most accurate method to measure NORM in a sample is to use a high resolution spectrometric instrument such as a germanium detector in a well-shielded laboratory environment. The shield is used to prevent background with the same signature from the building material of the laboratory. There are occasions in which one is required to assay samples in the field. These in situ field applications may require performing measurements with reduced (or no) background shielding conditions, or involve the use of medium resolution spectrometric instruments such as LaBr 3 or NaI detectors. In-situ analyses such as these have increased complexity. The reduced shielding enforces the subtraction of NORM events produced from the environment but the sample material and container can also shield the detector against this background thus biasing the measured results if not appropriately accounted. The use of medium resolution detectors has additional complications that the multiplicity of gamma-rays from NORM materials is such that most of the gamma-rays are interfering and thus require a very careful quantitative analysis. In this presentation, we will discuss the details of the NORM source term both in the environment and what could potentially be in the sample. We will also discuss
Lim, K.B.; Wennekes, J.; Jong, de J.H.S.G.M.; Jacobsen, E.; Tuyl, van J.M.
2001-01-01
Detailed karyotypes of Lilium longiflorum and L. rubellum were constructed on the basis of chromosome arm lengths, C-banding, AgNO3 staining, and PI-DAPI banding, together with fluorescence in situ hybridisation (FISH) with the 5S and 45S rDNA sequences as probes. The C-banding patterns that were
McInnes, B.; Danišík, M.; Evans, N.; McDonald, B.; Becker, T.; Vermeesch, P.
2015-12-01
We present a new laser-based technique for rapid, quantitative and automated in situ microanalysis of U, Th, Sm, Pb and He for applications in geochronology, thermochronometry and geochemistry (Evans et al., 2015). This novel capability permits a detailed interrogation of the time-temperature history of rocks containing apatite, zircon and other accessory phases by providing both (U-Th-Sm)/He and U-Pb ages (+trace element analysis) on single crystals. In situ laser microanalysis offers several advantages over conventional bulk crystal methods in terms of safety, cost, productivity and spatial resolution. We developed and integrated a suite of analytical instruments including a 193 nm ArF excimer laser system (RESOlution M-50A-LR), a quadrupole ICP-MS (Agilent 7700s), an Alphachron helium mass spectrometry system and swappable flow-through and ultra-high vacuum analytical chambers. The analytical protocols include the following steps: mounting/polishing in PFA Teflon using methods similar to those adopted for fission track etching; laser He extraction and analysis using a 2 s ablation at 5 Hz and 2-3 J/cm2fluence; He pit volume measurement using atomic force microscopy, and U-Th-Sm-Pb (plus optional trace element) analysis using traditional laser ablation methods. The major analytical challenges for apatite include the low U, Th and He contents relative to zircon and the elevated common Pb content. On the other hand, apatite typically has less extreme and less complex zoning of parent isotopes (primarily U and Th). A freeware application has been developed for determining (U-Th-Sm)/He ages from the raw analytical data and Iolite software was used for U-Pb age and trace element determination. In situ double-dating has successfully replicated conventional U-Pb and (U-Th)/He age variations in xenocrystic zircon from the diamondiferous Ellendale lamproite pipe, Western Australia and increased zircon analytical throughput by a factor of 50 over conventional methods
PPLA-cellulose nanocrystals nanocomposite prepared by in situ polymerization
International Nuclear Information System (INIS)
Paula, Everton L. de; Pereirea, Fabiano V.; Mano, Valdir
2011-01-01
This work reports the preparation and and characterization of a PLLA-cellulose nanocrystals nanocomposite obtained by in situ polymerization. The nanocomposite was prepared by ring opening polymerization of the lactide dimer in the presence of cellulose nanocrystals (CNCs) and the as-obtained materials was characterized using FTIR, DSC, XRD and TGA measurements. The incorporation of cellulose nanocrystals in PLLA using this method improved the thermal stability and increased the crystallinity of PLLA. These results indicate that the incorporation of CNCs by in situ polymerization improve thermal properties and has potential to improve also mechanical properties of this biodegradable polymer. (author)
Engineering feasibility analysis for in-situ stabilization of Canonsburg residues. [UMTRA project
Energy Technology Data Exchange (ETDEWEB)
1982-01-01
The US Department of Energy is considering several methods for carrying out remedial actions in Canonsburg, Pennsylvania, at the site of an inactive uranium-processing mill. The main objective of this study is to determine the feasibility of in-situ stabilization as the remedial action. In-situ stabilization is an alternative to site decontamination and offsite disposal. The problems associated with offsite hauling of large quantities of contaminated material and with the location and development of a new disposal site could be avoided by the implementation of an in-situ stabilization concept. In addition, the in-situ approach would be more cost-effective than offsite disposal. This study will establish that a technically feasible and implementable in-situ stabilization concept can be developed that meets regulatory requirements and is cost effective. This study in no way commits the DOE to implement any specific actions described herein. 11 refs., 30 figs., 24 tabs.
Directory of Open Access Journals (Sweden)
Simon Jon McIlroy
Full Text Available An in depth understanding of the ecology of activated sludge nutrient removal wastewater treatment systems requires detailed knowledge of the community composition and metabolic activities of individual members. Recent 16S rRNA gene amplicon surveys of activated sludge wastewater treatment plants with nutrient removal indicate the presence of a core set of bacterial genera. These organisms are likely responsible for the bulk of nutrient transformations underpinning the functions of these plants. While the basic activities of some of these genera in situ are known, there is little to no information for the majority. This study applied microautoradiography coupled with fluorescence in situ hybridization (MAR-FISH for the in situ characterization of selected genus-level-phylotypes for which limited physiological information is available. These included Sulfuritalea and A21b, both within the class Betaproteobacteria, as well as Kaga01, within sub-group 10 of the phylum Acidobacteria. While the Sulfuritalea spp. were observed to be metabolically versatile, the A21b and Kaga01 phylotypes appeared to be highly specialized.
Estimation of In-Situ Groundwater Conditions Based on Geochemical Equilibrium Simulations
Directory of Open Access Journals (Sweden)
Toshiyuki Hokari
2014-03-01
Full Text Available This paper presents a means of estimating in-situ groundwater pH and oxidation-redox potential (ORP, two very important parameters for species migration analysis in safety assessments for radioactive waste disposal or carbon dioxide sequestration. The method was applied to a pumping test in a deep borehole drilled in a tertiary formation in Japan for validation. The following application examples are presented: when applied to several other pumping tests at the same site, it could estimate distributions of the in-situ groundwater pH and ORP; applied to multiple points selected in the groundwater database of Japan, it could help estimate the in-situ redox reaction governing the groundwater conditions in some areas.
Cartwright, Grace M.; Friedrichs, Carl T.; Smith, S. Jarrell
2013-12-01
Under conditions common in muddy coastal and estuarine environments, acoustic Doppler velocimeters (ADVs) can serve to estimate sediment settling velocity ( w s) by assuming a balance between upward turbulent Reynolds flux and downward gravitational settling. Advantages of this method include simple instrument deployment, lack of flow disturbance, and relative insensitivity to biofouling and water column stratification. Although this method is being used with increasing frequency in coastal and estuarine environments, to date it has received little direct ground truthing. This study compared in situ estimates of w s inferred by a 5-MHz ADV to independent in situ observations from a high-definition video settling column over the course of a flood tide in the bottom boundary layer of the York River estuary, Virginia, USA. The ADV-based measurements were found to agree with those of the settling column when the current speed at about 40 cm above the bed was greater than about 20 cm/s. This corresponded to periods when the estimated magnitude of the settling term in the suspended sediment continuity equation was four or more times larger than the time rate of change of concentration. For ADV observations restricted to these conditions, ADV-based estimates of w s (mean 0.48±0.04 mm/s) were highly consistent with those observed by the settling column (mean 0.45±0.02 mm/s). However, the ADV-based method for estimating w s was sensitive to the prescribed concentration of the non-settling washload, C wash. In an objective operational definition, C wash can be set equal to the lowest suspended solids concentration observed around slack water.
He, Yongrui; Zhao, Xian-En; Zhu, Shuyun; Wei, Na; Sun, Jing; Zhou, Yubi; Liu, Shu; Liu, Zhiqiang; Chen, Guang; Suo, Yourui; You, Jinmao
2016-08-05
Simultaneous monitoring of several neurotransmitters (NTs) linked to Parkinson's disease (PD) has important scientific significance for PD related pathology, pharmacology and drug screening. A new simple, fast and sensitive analytical method, based on in situ derivatization-ultrasound-assisted dispersive liquid-liquid microextraction (in situ DUADLLME) in a single step, has been proposed for the quantitative determination of catecholamines and their biosynthesis precursors and metabolites in rat brain microdialysates. The method involved the rapid injection of the mixture of low toxic bromobenzene (extractant) and acetonitrile (dispersant), which containing commercial Lissamine rhodamine B sulfonyl chloride (LRSC) as derivatization reagent, into the aqueous phase of sample and buffer, and the following in situ DUADLLME procedure. After centrifugation, 50μL of the sedimented phase (bromobenzene) was directly injected for ultra high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) detection in multiple reaction monitoring (MRM) mode. This interesting combination brought the advantages of speediness, simpleness, low matrix effects and high sensitivity in an effective way. Parameters of in situ DUADLLME and UHPLC-MS/MS conditions were all optimized in detail. The optimum conditions of in situ DUADLLME were found to be 30μL of microdialysates, 150μL of acetonitrile containing LRSC, 50μL of bromobenzene and 800μL of NaHCO3-Na2CO3 buffer (pH 10.5) for 3.0min at 37°C. Under the optimized conditions, good linearity was observed with LODs (S/N>3) and LOQs (S/N>10) of LRSC derivatized-NTs in the range of 0.002-0.004 and 0.007-0.015 nmol/L, respectively. It also brought good precision (3.2-12.8%, peak area CVs%), accuracy (94.2-108.6%), recovery (94.5-105.5%) and stability (3.8-8.1%, peak area CVs%) results. Moreover, LRSC derivatization significantly improved chromatographic resolution and MS detection sensitivity of NTs when compared with the
Using the in situ lift-out technique to prepare TEM specimens on a single-beam FIB instrument
International Nuclear Information System (INIS)
Lekstrom, M; McLachlan, M A; Husain, S; McComb, D W; Shollock, B A
2008-01-01
Transmission electron microscope (TEM) specimens are today routinely prepared using focussed ion beam (FIB) instruments. Specifically, the lift-out method has become an increasingly popular technique and involves removing thin cross-sections from site-specific locations and transferring them to a TEM grid. This lift-out process can either be performed ex situ or in situ. The latter is mainly carried out on combined dual-beam FIB and scanning electron microscope (SEM) systems whereas conventional single-beam instruments often are limited to the traditional ex situ method. It is nevertheless desirable to enhance the capabilities of existing single-beam instruments to allow for in situ lift-out preparation to be performed since this technique offers a number of advantages over the older ex situ method. A single-beam FIB instrument was therefore modified to incorporate an in situ micromanipulator fitted with a tungsten needle, which can be attached to a cut-out FIB section using ion beam induced platinum deposition. This article addresses the issues of using an ion beam to monitor the in situ manipulation process as well as approaches that can be used to create stronger platinum welds between two objects, and finally, views on how to limit the extent of ion beam damage to the specimen surface.
Energy Technology Data Exchange (ETDEWEB)
Li, Boyan; Ou, Longwen; Dang, Qi; Meyer, Pimphan A.; Jones, Susanne B.; Brown, Robert C.; Wright, Mark
2015-11-01
This study evaluates the techno-economic uncertainty in cost estimates for two emerging biorefinery technologies for biofuel production: in situ and ex situ catalytic pyrolysis. Stochastic simulations based on process and economic parameter distributions are applied to calculate biorefinery performance and production costs. The probability distributions for the minimum fuel-selling price (MFSP) indicate that in situ catalytic pyrolysis has an expected MFSP of $4.20 per gallon with a standard deviation of 1.15, while the ex situ catalytic pyrolysis has a similar MFSP with a smaller deviation ($4.27 per gallon and 0.79 respectively). These results suggest that a biorefinery based on ex situ catalytic pyrolysis could have a lower techno-economic risk than in situ pyrolysis despite a slightly higher MFSP cost estimate. Analysis of how each parameter affects the NPV indicates that internal rate of return, feedstock price, total project investment, electricity price, biochar yield and bio-oil yield are significant parameters which have substantial impact on the MFSP for both in situ and ex situ catalytic pyrolysis.
In situ nanoparticle diagnostics by multi-wavelength Rayleigh-Mie scattering ellipsometry
Gebauer, G
2003-01-01
We present and discuss the method of multiple-wavelength Rayleigh-Mie scattering ellipsometry for the in situ analysis of nanoparticles. It is applied to the problem of nanoparticles suspended in low-pressure plasmas. We discuss experimental results demonstrating that the size distribution and the complex refractive index can be determined with high accuracy and present a study on the in situ analysis of etching of melamine-formaldehyde nanoparticles suspended in an oxygen plasma. It is also shown that particles with a shell structure (core plus mantle) can be analysed by Rayleigh-Mie scattering ellipsometry. Rayleigh-Mie scattering ellipsometry is also applicable to in situ analysis of nanoparticles under high gas pressures and in liquids.
In Situ Study of Thermal Stability of Copper Oxide Nanowires at Anaerobic Environment
Directory of Open Access Journals (Sweden)
Lihui Zhang
2014-01-01
Full Text Available Many metal oxides with promising electrochemical properties were developed recently. Before those metal oxides realize the use as an anode in lithium ion batteries, their thermal stability at anaerobic environment inside batteries should be clearly understood for safety. In this study, copper oxide nanowires were investigated as an example. Several kinds of in situ experiment methods including in situ optical microscopy, in situ Raman spectrum, and in situ transmission electron microscopy were adopted to fully investigate their thermal stability at anaerobic environment. Copper oxide nanowires begin to transform as copper(I oxide at about 250°C and finish at about 400°C. The phase transformation proceeds with a homogeneous nucleation.
DEFF Research Database (Denmark)
Dige, Irene; Kilian, Mogens; Nilsson, Holger
2007-01-01
Confocal laser scanning microscopy (CLSM) has been employed as a method for studying intact natural biofilm. When combined with fluorescence in situ hybridization (FISH) it is possible to analyze spatial relationships and changes of specific members of microbial populations over time. The aim...
A New Method for in Situ Measurement of Bt-Maize Pollen Deposition on Host-Plant Leaves
Directory of Open Access Journals (Sweden)
Rudolph Vögel
2011-02-01
Full Text Available Maize is wind pollinated and produces huge amounts of pollen. In consequence, the Cry toxins expressed in the pollen of Bt maize will be dispersed by wind in the surrounding vegetation leading to exposure of non-target organisms (NTO. NTO like lepidopteran larvae may be affected by the uptake of Bt-pollen deposited on their host plants. Although some information is available to estimate pollen deposition on host plants, recorded data are based on indirect measurements such as shaking or washing off pollen, or removing pollen with adhesive tapes. These methods often lack precision and they do not include the necessary information such as the spatial and temporal variation of pollen deposition on the leaves. Here, we present a new method for recording in situ the amount and the distribution of Bt-maize pollen deposited on host plant leaves. The method is based on the use of a mobile digital microscope (Dino-Lite Pro, including DinoCapture software, which can be used in combination with a notebook in the field. The method was evaluated during experiments in 2008 to 2010. Maize pollen could be correctly identified and pollen deposition as well as the spatial heterogeneity of maize pollen deposition was recorded on maize and different lepidopteran host plants (Centaurea scabiosa, Chenopodium album, Rumex spp., Succina pratensis and Urtica dioica growing adjacent to maize fields.
DEFF Research Database (Denmark)
Lemming, Gitte; Nielsen, Steffen G.; Weber, Klaus
2013-01-01
In situ thermal remediation technologies provide efficient and reliable cleanup of contaminated soil and groundwater, but at a high cost of environmental impacts and resource depletion due to the large amounts of energy and materials consumed. This study provides a detailed investigation of four...... in situ thermal remediation technologies (steam enhanced extraction, thermal conduction heating, electrical resistance heating, and radio frequency heating) in order to (1) compare the life-cycle environmental impacts and resource consumption associated with each thermal technology, and (2) identify...... improvements is a 10 to 21% decrease in environmental impacts and an 8 to 20% decrease in resource depletion depending on the thermal remediation technology considered. The energy consumption was found to be the main contributor to most types of environmental impacts; this will, however, depend...
International Nuclear Information System (INIS)
Shapiro, A.P.; Sivavec, T.M.; Baghel, S.S.
1997-01-01
Contamination in low-permeability soils poses a significant technical challenge for in situ remediation efforts. Poor accessibility to the contaminants and difficulty in delivery of treatment reagents have rendered existing in situ treatments such as bioremediation, vapor extraction, pump and treat rather ineffective when applied to low-permeability soils present at many contaminated sites. The technology is an integrated in situ treatment in which established geotechnical methods are used to install degradation zones directly in the contaminated soil and electro-osmosis is used to move the contaminants back and forth through those zones until the treatment is completed. The present Draft Topical Report for Task No. 9 summarizes laboratory investigations into TCE degradation using nonbiological methods. These studies were conducted by the General Electric Company. The report concentrates on zero valent iron as the reducing agent and presents data on TCE and daughter product degradation rates in batch experiments, column studies, and electroosmotic cells. It is shown that zero valent iron effectively degrades TCE in electroosmotic experiments. Daughter product degradation and gas generation are shown to be important factors in designing field scale treatment zones for the Lasagna trademark process
In-situ observation of atomic self-organization processes in Xe nanocrystals embedded in Al
International Nuclear Information System (INIS)
Mitsuishi, K.; Song, M.; Furuya, K.; Birtcher, R. C.; Allen, C. W.; Donnelly, S. E.
1998-01-01
Self-organization processes in Xe nanocrystals embedded in Al are observed with in-situ high-resolution electron microscopy. Under electron irradiation, stacking fault type defects are produced in Xe nanocrystals. The defects recover in a layer by layer manner. Detailed analysis of the video reveals that the displacement of Xe atoms in the stacking fault was rather small for the Xe atoms at boundary between Xe and Al, suggesting the possibility of the stacking fault in Xe precipitate originating inside of precipitate, not at the Al/Xe interface
In meso in situ serial X-ray crystallography of soluble and membrane proteins
International Nuclear Information System (INIS)
Huang, Chia-Ying; Olieric, Vincent; Ma, Pikyee; Panepucci, Ezequiel; Diederichs, Kay; Wang, Meitian; Caffrey, Martin
2015-01-01
A method for performing high-throughput in situ serial X-ray crystallography with soluble and membrane proteins in the lipid cubic phase is described. It works with microgram quantities of protein and lipid (and ligand when present) and is compatible with the most demanding sulfur SAD phasing. The lipid cubic phase (LCP) continues to grow in popularity as a medium in which to generate crystals of membrane (and soluble) proteins for high-resolution X-ray crystallographic structure determination. To date, the PDB includes 227 records attributed to the LCP or in meso method. Among the listings are some of the highest profile membrane proteins, including the β 2 -adrenoreceptor–G s protein complex that figured in the award of the 2012 Nobel Prize in Chemistry to Lefkowitz and Kobilka. The most successful in meso protocol to date uses glass sandwich crystallization plates. Despite their many advantages, glass plates are challenging to harvest crystals from. However, performing in situ X-ray diffraction measurements with these plates is not practical. Here, an alternative approach is described that provides many of the advantages of glass plates and is compatible with high-throughput in situ measurements. The novel in meso in situ serial crystallography (IMISX) method introduced here has been demonstrated with AlgE and PepT (alginate and peptide transporters, respectively) as model integral membrane proteins and with lysozyme as a test soluble protein. Structures were solved by molecular replacement and by experimental phasing using bromine SAD and native sulfur SAD methods to resolutions ranging from 1.8 to 2.8 Å using single-digit microgram quantities of protein. That sulfur SAD phasing worked is testament to the exceptional quality of the IMISX diffraction data. The IMISX method is compatible with readily available, inexpensive materials and equipment, is simple to implement and is compatible with high-throughput in situ serial data collection at macromolecular
Continuous in-situ measurements of fission fragment irradiation induced void swelling in Ni
International Nuclear Information System (INIS)
Lefakis, H.
1980-01-01
A novel simulation technique has been developed to study the early stages of irradiation induced void formation in metals. The technique makes use of fission fragment irradiation produced by doping with 235 U and irradiating in a thermal neutron flux under highly controlled irradiation-environmental conditions. Employment of a computer and a high temperature radiation resistant LVDT resulted in a high volumetric sensitivity and the production of continuous, in-situ void swelling data for bulk specimens. Results for Ni, used as a test-metal served to corroborate the technique in a number of ways including comparisons with (a) reactor data, (b) direct post-irradiation specimen length measurements and (c) TEM examinations of irradiated samples. The technique has several unique advantages and, in conjunction with other conventional methods, it offers the possibility of detailed evaluation of void nucleation and growth theories. In view of the present results no definitive answer may be given on the issue of the incubation period while checks with two theoretical models have yielded an order-of-magnitude agreement
Wu, Yimin A.; Yin, Zuwei; Farmand, Maryam; Yu, Young-Sang; Shapiro, David A.; Liao, Hong-Gang; Liang, Wen-I.; Chu, Ying-Hao; Zheng, Haimei
2017-02-01
We report the study of Mg cathodic electrochemical deposition on Ti and Au electrode using a multimodal approach by examining the sample area in-situ using liquid cell transmission electron microscopy (TEM), scanning transmission X-ray microscopy (STXM) and X-ray absorption spectroscopy (XAS). Magnesium Aluminum Chloride Complex was synthesized and utilized as electrolyte, where non-reversible features during in situ charging-discharging cycles were observed. During charging, a uniform Mg film was deposited on the electrode, which is consistent with the intrinsic non-dendritic nature of Mg deposition in Mg ion batteries. The Mg thin film was not dissolvable during the following discharge process. We found that such Mg thin film is hexacoordinated Mg compounds by in-situ STXM and XAS. This study provides insights on the non-reversibility issue and failure mechanism of Mg ion batteries. Also, our method provides a novel generic method to understand the in situ battery chemistry without any further sample processing, which can preserve the original nature of battery materials or electrodeposited materials. This multimodal in situ imaging and spectroscopy provides many opportunities to attack complex problems that span orders of magnitude in length and time scale, which can be applied to a broad range of the energy storage systems.
International Nuclear Information System (INIS)
Hatfield, K.
2003-01-01
Tracer method to establish presence and distribution of chromium reducing microbes. The primary objective of this research was to establish an in situ tracer method for detecting the presence. distribution. and activity of subsurface heavy metal-reducing microorganisms. Research focused on microbial systems responsible for the reduction of chromium and a suite of biotracers coupled to the reduction process. The tracer method developed may be used to characterize sites contaminated with chromium or expedite bioremediation: and although research focused on chromium. the method can be easily extended to other metals, organics, and radionuclides. This brief final report contains three major sections. The first identifies specific products of the research effort such as students supported and publications. The second section briefly presents major research findings, while the last section summarizes the overall research effort
Energy Technology Data Exchange (ETDEWEB)
Hatfield, K.
2003-07-01
Tracer method to establish presence and distribution of chromium reducing microbes. The primary objective of this research was to establish an in situ tracer method for detecting the presence. distribution. and activity of subsurface heavy metal-reducing microorganisms. Research focused on microbial systems responsible for the reduction of chromium and a suite of biotracers coupled to the reduction process. The tracer method developed may be used to characterize sites contaminated with chromium or expedite bioremediation: and although research focused on chromium. the method can be easily extended to other metals, organics, and radionuclides. This brief final report contains three major sections. The first identifies specific products of the research effort such as students supported and publications. The second section briefly presents major research findings, while the last section summarizes the overall research effort.
International Nuclear Information System (INIS)
Heath, W.O.
1987-04-01
A method for measuring the in situ flow properties of high-level radioactive waste (HLW) sludges has been developed at Pacific Northwest Laboratory, along with a preconceptual design for a shear vane device that can be installed in underground HLW storage tanks and used to make those measurements remotely. The data obtained with this device will assist in the design of mixing equipment used to resuspend and remove HLW sludges from their storage tanks for downstream processing. This method is also suitable for remotely characterizing other types of waste sludges and slurries. Commonly available viscometric methods were adapted to allow characterization of sludge samples in the laboratory such that the laboratory and in-tank data can be directly compared (scaled up). Procedures for conducting measurements and analyzing the results in terms of useful mathematical models describing both start-up and steady-state flow behavior are presented, as is a brief tutorial on the types of flow behavior that can be exhibited by tank sludges. 30 refs., 36 figs., 14 tabs
In-Situ Creep Monitoring Using the Potential Drop Method
Madhi, E.; Sposito, G.; Davies, C. M.; Cawley, P.; Nagy, P. B.
2011-06-01
A directional low-frequency Alternating Current Potential Drop (ACPD) sensor was developed for in-situ monitoring of creep in metals. The sensor relies on a modified ACPD technique that measures simultaneously both values of resistance in the axial and lateral directions using a square electrode configuration. As compared to the more commonly used in-line electrode configuration, the square arrangement used in this sensor is much more directional and thus allows the detection of creep-induced anisotropy and texture. The technique monitors the variation in the ratio of the measured axial and lateral resistances, therefore can efficiently separate the mostly isotropic common part of the resistivity variation caused by reversible temperature variations from the mostly anisotropic differential part caused by direct geometrical and indirect material effects of creep. Initially, this ratio is roughly proportional to the axial creep strain, while at later stages, the resistance ratio increases even faster with creep strain because of the formation of directional discontinuities such as preferentially oriented grain boundary cavities and multiple-site microcracks in the material.
In situ and ex situ modifications of bacterial cellulose for applications in tissue engineering.
Stumpf, Taisa Regina; Yang, Xiuying; Zhang, Jingchang; Cao, Xudong
2018-01-01
Bacterial cellulose (BC) is secreted by a few strains of bacteria and consists of a cellulose nanofiber network with unique characteristics. Because of its excellent mechanical properties, outstanding biocompatibilities, and abilities to form porous structures, BC has been studied for a variety of applications in different fields, including the use as a biomaterial for scaffolds in tissue engineering. To extend its applications in tissue engineering, native BC is normally modified to enhance its properties. Generally, BC modifications can be made by either in situ modification during cell culture or ex situ modification of existing BC microfibers. In this review we will first provide a brief introduction of BC and its attributes; this will set the stage for in-depth and up-to-date discussions on modified BC. Finally, the review will focus on in situ and ex situ modifications of BC and its applications in tissue engineering, particularly in bone regeneration and wound dressing. Copyright © 2016. Published by Elsevier B.V.
In-situ CdS/CdTe Heterojuntions Deposited by Pulsed Laser Deposition
Avila-Avendano, Jesus
2016-04-09
In this paper pulsed laser deposition (PLD) methods are used to study p-n CdTe/CdS heterojunctions fabricated in-situ. In-situ film deposition allows higher quality p-n interfaces by minimizing spurious contamination from the atmosphere. Morphologic and structural analyses were carried for CdTe films deposited on various substrates and different deposition conditions. The electrical characteristics and performance of the resulting p-n heterojunctions were studied as function of substrate and post-deposition anneal temperature. In-situ growth results on diodes with a rectification factor of ~ 105, an ideality factor < 2, and a reverse saturation current ~ 10-8 A. The carrier concentration in the CdTe film was in the range of ~ 1015 cm-3, as measured by C-V methods. The possible impact of sulfur diffusion from the CdS into the CdTe film is also investigated using High Resolution Rutherford Back-Scattering.
In-situ CdS/CdTe Heterojuntions Deposited by Pulsed Laser Deposition
Avila-Avendano, Jesus; Mejia, Israel; Alshareef, Husam N.; Guo, Zaibing; Young, Chadwin; Quevedo-Lopez, Manuel
2016-01-01
In this paper pulsed laser deposition (PLD) methods are used to study p-n CdTe/CdS heterojunctions fabricated in-situ. In-situ film deposition allows higher quality p-n interfaces by minimizing spurious contamination from the atmosphere. Morphologic and structural analyses were carried for CdTe films deposited on various substrates and different deposition conditions. The electrical characteristics and performance of the resulting p-n heterojunctions were studied as function of substrate and post-deposition anneal temperature. In-situ growth results on diodes with a rectification factor of ~ 105, an ideality factor < 2, and a reverse saturation current ~ 10-8 A. The carrier concentration in the CdTe film was in the range of ~ 1015 cm-3, as measured by C-V methods. The possible impact of sulfur diffusion from the CdS into the CdTe film is also investigated using High Resolution Rutherford Back-Scattering.
Design of Hybrid Steam-In Situ Combustion Bitumen Recovery Processes
International Nuclear Information System (INIS)
Yang Xiaomeng; Gates, Ian D.
2009-01-01
Given enormous capital costs, operating expenses, flue gas emissions, water treatment and handling costs of thermal in situ bitumen recovery processes, improving the overall efficiency by lowering energy requirements, environmental impact, and costs of these production techniques is a priority. Steam-assisted gravity drainage (SAGD) is the most widely used in situ recovery technique in Athabasca reservoirs. Steam generation is done on surface and consequently, because of heat losses, the energy efficiency of SAGD can never be ideal with respect to the energy delivered to the sandface. An alternative to surface steam generation is in situ combustion (ISC) where heat is generated within the formation through injection of oxygen at a sufficiently high pressure to initiate combustion of bitumen. In this manner, the heat from the combustion reactions can be used directly to mobilize the bitumen. As an alternative, the heat can be used to generate steam within the formation which then is the agent to move heat in the reservoir. In this research, alternative hybrid techniques with simultaneous and sequential steam-oxygen injection processes are examined to maximize the thermal efficiency of the recovery process. These hybrid processes have the advantage that during ISC, steam is generated within the reservoir from injected and formation water and as a product of oxidation. This implies that ex situ steam generation requirements are reduced and if there is in situ storage of combustion gases, that overall gas emissions are reduced. In this research, detailed reservoir simulations are done to examine the dynamics of hybrid processes to enable design of these processes. The results reveal that hybrid processes can lower emitted carbon dioxide-to-oil ratio by about 46%, decrease the consumed natural gas-to-oil ratio by about 73%, reduce the cumulative energy-to-oil ratio by between 40% and 70% compared to conventional SAGD, and drop water consumption per unit oil produced
In situ synthesis and modification of calcium carbonate nanoparticles via a bobbling method
Institute of Scientific and Technical Information of China (English)
无
2009-01-01
Modified calcium carbonate (CaCO3) nanoparticles with cubic- and spindle-like configuration were synthesized in situ by the typical bobbling (gas-liquid-solid) method. The modifiers, such as sodium stearate, octadecyl dihydrogen phosphate (ODP) and oleic acid (OA), were used to obtain hydrophobic nanoparticles. The different modification effects of the modifiers were investigated by measuring the active ratio, whiteness and the contact angle. Moreover, transmission electron microscopy (TEM), X-ray diffraction (XRD) and thermogravimetry analysis (TGA analysis) were employed to characterize the obtained products. A preliminary reaction mechanism was discussed. According to the results, the active ratio of CaCO3 modified by ODP was ca. 99.9% and the value of whiteness was 97.3% when the dosage of modifiers reached 2%. The contact angle was 122.25° for the CaCO3 modified in the presence of sodium stearate, ODP and OA. When modified CaCO3 was filled into PVC, the mechanical properties of products were improved greatly such as rupture intensity, pull intensity and fuse temperature. The compatibility and affinity between the modified CaCO3 nanoparticles and the organic matrixes were greatly improved.
In situ synthesis and modification of calcium carbonate nanoparticles via a bobbling method
Institute of Scientific and Technical Information of China (English)
ZHAO LiNa; FENG JingDong; WANG ZiChen
2009-01-01
Modified calcium carbonate (CaCO3) nanoparticles with cubic- and spindle-like configuration were synthesized in situ by the typical bobbling (gas-liquid-solid) method.The modifiers,such as sodium stearate,octadecyl dihydrogen phosphate (ODP) and oleic acid (OA),were used to obtain hydrophobic nanoparticles.The different modification effects of the modifiers were investigated by measuring the active ratio,whiteness and the contact angle.Moreover,transmission electron microscopy (TEM),X-ray diffraction (XRD) and thermogravimetry analysis (TGA analysis) were employed to characterize the obtained products.A preliminary reaction mechanism was discussed.According to the results,the active ratio of CaCO3 modified by ODP was ca.99.9% and the value of whiteness was 97.3% when the dosage of modifiers reached 2%.The contact angle was 122.25° for the CaCO3 modified in the presence of sodium stearate,ODP and OA.When modified CaCO3 was filled into PVC,the mechanical properties of products were improved greatly such as rupture intensity,pull intensity and fuse temperature.The compatibility and affinity between the modified CaCO3 nanoparticles and the organic matrixes were greatly improved.
Advanced approach to the analysis of a series of in-situ nuclear forward scattering experiments
Energy Technology Data Exchange (ETDEWEB)
Vrba, Vlastimil, E-mail: vlastimil.vrba01@upol.cz [Department of Experimental Physics, Faculty of Science, Palacký University Olomouc, 17. listopadu 12, 771 46 Olomouc (Czech Republic); Procházka, Vít, E-mail: v.prochazka@upol.cz [Department of Experimental Physics, Faculty of Science, Palacký University Olomouc, 17. listopadu 12, 771 46 Olomouc (Czech Republic); Smrčka, David, E-mail: david.smrcka@upol.cz [Department of Experimental Physics, Faculty of Science, Palacký University Olomouc, 17. listopadu 12, 771 46 Olomouc (Czech Republic); Miglierini, Marcel, E-mail: marcel.miglierini@stuba.sk [Slovak University of Technology in Bratislava, Faculty of Electrical Engineering and Information Technology, Institute of Nuclear and Physical Engineering, Ilkovicova 3, 812 19 Bratislava (Slovakia); Department of Nuclear Reactors, Faculty of Nuclear Science and Physical Engineering, Czech Technical University in Prague, V Holešovičkách 2, 180 00 Prague (Czech Republic)
2017-03-01
This study introduces a sequential fitting procedure as a specific approach to nuclear forward scattering (NFS) data evaluation. Principles and usage of this advanced evaluation method are described in details and its utilization is demonstrated on NFS in-situ investigations of fast processes. Such experiments frequently consist of hundreds of time spectra which need to be evaluated. The introduced procedure allows the analysis of these experiments and significantly decreases the time needed for the data evaluation. The key contributions of the study are the sequential use of the output fitting parameters of a previous data set as the input parameters for the next data set and the model suitability crosscheck option of applying the procedure in ascending and descending directions of the data sets. Described fitting methodology is beneficial for checking of model validity and reliability of obtained results.
Advanced approach to the analysis of a series of in-situ nuclear forward scattering experiments
International Nuclear Information System (INIS)
Vrba, Vlastimil; Procházka, Vít; Smrčka, David; Miglierini, Marcel
2017-01-01
This study introduces a sequential fitting procedure as a specific approach to nuclear forward scattering (NFS) data evaluation. Principles and usage of this advanced evaluation method are described in details and its utilization is demonstrated on NFS in-situ investigations of fast processes. Such experiments frequently consist of hundreds of time spectra which need to be evaluated. The introduced procedure allows the analysis of these experiments and significantly decreases the time needed for the data evaluation. The key contributions of the study are the sequential use of the output fitting parameters of a previous data set as the input parameters for the next data set and the model suitability crosscheck option of applying the procedure in ascending and descending directions of the data sets. Described fitting methodology is beneficial for checking of model validity and reliability of obtained results.
In situ calibration of inductively coupled plasma-atomic emission and mass spectroscopy
Braymen, Steven D.
1996-06-11
A method and apparatus for in situ addition calibration of an inductively coupled plasma atomic emission spectrometer or mass spectrometer using a precision gas metering valve to introduce a volatile calibration gas of an element of interest directly into an aerosol particle stream. The present situ calibration technique is suitable for various remote, on-site sampling systems such as laser ablation or nebulization.
PETher - Physical Properties of Thermal Water under In-situ-Conditions
Herfurth, Sarah; Schröder, Elisabeth
2016-04-01
The objective of PETher, a research project funded by the German Federal Ministry for Economic Affairs and Energy (BMWi), is to experimentally determine thermo-physical properties (specific isobaric heat capacity, kinematic viscosity, density and thermal conductivity) of geothermal water in-situ-conditions (pressure, temperature, chemical composition including gas content of the brine) present in geothermal applications. Knowing these thermo-physical properties reduces the uncertainties with respect to estimating the thermal output and therefore the economic viability of the power plant. Up to now, only a limited number of measurements of selected physical properties have been made, usually under laboratory conditions and for individual geothermal plants. In-situ measured parameters, especially in the temperature range of 120°C and higher, at pressures of 20 bar and higher, as well as with a salinity of up to 250 g/l, are sparse to non-existing. Therefore, pure water properties are often used as reference data and for designing the power plant and its components. Currently available numerical models describing the thermo-physical properties are typically not valid for the conditions in geothermal applications and do not consider the substantial influence of the chemical composition of the thermal water. Also, actual geothermal waters have not been subject of detailed measurements systematically performed under operational conditions on a large-scale basis. Owing to the lack of reliable data, a validation of numerical models for investigating geothermal systems is not possible. In order to determine the dependency of the thermo-physical properties of geothermal water on temperature, pressure and salinity in-situ measurements are conducted. The measurements are taking place directly at several geothermal applications located in Germany's hydrogeothermal key regions. In order to do this, a mobile testing unit was developed and refined with instruments specifically
Preparation of Biodiesel of Undi seed with In-situ Transesterification
Directory of Open Access Journals (Sweden)
Sanjaykumar DALVI
2012-08-01
Full Text Available The biodiesel fraction from oil content of Undi (Calophyllum innophyllum L. is found 60-70%. The extraction of oil is a primary step in any biodiesel production system. To escape this step in-situ transesterification method is used in which the Undi seed crush is directly converted into biodiesel with in-situ transesterification which is fatty acid methyl and ethyl ester composition. The single step reaction is eco-friendly as hexane like solvents not have been used for oil extraction. These components of biodiesel were analysed by GC-MS technique.
In situ conditioning for proton storage ring vacuum systems
International Nuclear Information System (INIS)
Blechschmidt, D.
1978-01-01
Average pressure and vacuum-stability limit as expected in the presence of a proton beam were measured after in situ treatments such as bakeout under various conditions, argon glow-discharge cleaning and sputter deposition of titanium. Measurements were carried out for test pipes made of stainless steel (untreated, electropolished, or cooled to 77 K), pure titanium and aluminum alloy. The measurement method used to obtain the vacuum-stability limit in the laboratory and in a prototype system is described. The results can be applied also to other systems of different geometry by use of scaling laws. In situ conditioning generally has a stronger influence on vacuum performance than a particular choice of material. Bakeout gives low average pressures and rather good vacuum stability. Glow discharges also increase the vacuum stability but have only a small effect on the static pressure. Coating the beam-pipe wall with titanium by in situ sputtering provides large linear pumping, thus a lower pressure and an extremely good vacuum stability
Energy Technology Data Exchange (ETDEWEB)
Koca, Atif, E-mail: akoca@eng.marmara.edu.tr [Department of Chemical Engineering, Faculty of Engineering, Marmara University, Goeztepe, 34722 Istanbul (Turkey); Kalkan, Ayfer; Bayir, Zehra Altuntas [Department of Chemistry, Technical University of Istanbul, Maslak, 34469 Istanbul (Turkey)
2011-06-30
Highlights: > Electrochemical and in situ spectroelectrochemical characterizations of the metallophthalocyanines were performed. > The presence of O{sub 2} influences both oxygen reduction reaction and the electrochemical behaviors of the complexes. > Homogeneous catalytic ORR process occurs via an 'inner sphere' chemical catalysis process. > CoPc and CuPc coated on a glassy carbon electrode decrease the overpotential of the working electrode for H{sup +} reduction. - Abstract: This study describes electrochemical, in situ spectroelectrochemical, and in situ electrocolorimetric monitoring of the electrocatalytic reduction of molecular oxygen and hydronium ion on the phthalocyanine-modified electrodes. For this purpose, electrochemical and in situ spectroelectrochemical characterizations of the metallophthalocyanines (MPc) bearing tetrakis-[4-((4'-trifluoromethyl)phenoxy)phenoxy] groups were performed. While CoPc gives both metal-based and ring-based redox processes, H{sub 2}Pc, ZnPc and CuPc show only ring-based electron transfer processes. In situ electrocolorimetric method was applied to investigate the color of the electrogenerated anionic and cationic forms of the complexes. The presence of O{sub 2} in the electrolyte system influences both oxygen reduction reaction and the electrochemical and spectral behaviors of the complexes, which indicate electrocatalytic activity of the complexes for the oxygen reduction reaction. Perchloric acid titrations monitored by voltammetry represent possible electrocatalytic activities of the complexes for hydrogen evolution reaction. CoPc and CuPc coated on a glassy carbon electrode decrease the overpotential of the working electrode for H{sup +} reduction. The nature of the metal center changes the electrocatalytic activities for hydrogen evolution reaction in aqueous solution. Although CuPc has an inactive metal center, its electrocatalytic activity is recorded more than CoPc for H{sup +} reduction in aqueous
International Nuclear Information System (INIS)
Mao, Wen-feng; Yan, Ji; Xie, Hui; Tang, Zhi-yuan; Xu, Qiang
2013-01-01
An in situ polymerization assisted fast sol–gel method was introduced to synthesize high performance Li 3 V 2 PO 4 /C (LVP/C) cathode material. The crystal structure, surface morphology and electrochemical performances of the LVP/C samples sintered at different temperatures were investigated. The composite sintered at 750 °C exhibits the highest specific discharge capacity of 119.02 mAh g −1 (440.35 Wh g −1 ) at 10 C rate. The Li + diffusion coefficient ranges from 10 −6 to 10 −8 cm 2 s −1 based on different scanning rates and the electronic conductivity is about 10 −5 S cm −1 . For comparison, an ex situ polymerization method was also employed to obtain the LVP/C composite. A novel charge/discharge testing mode was designed to investigate the electrochemical behavior of the as-prepared LVP/C composite for practical application in electric vehicle cells. The obtained high power density and the special testing mode prove the LVP/C composite would be a promising candidate for the electric vehicle application and deserves further investigation
Label-free in situ imaging of oil body dynamics and chemistry in germination.
Waschatko, Gustav; Billecke, Nils; Schwendy, Sascha; Jaurich, Henriette; Bonn, Mischa; Vilgis, Thomas A; Parekh, Sapun H
2016-10-01
Plant oleosomes are uniquely emulsified lipid reservoirs that serve as the primary energy source during seed germination. These oil bodies undergo significant changes regarding their size, composition and structure during normal seedling development; however, a detailed characterization of these oil body dynamics, which critically affect oil body extractability and nutritional value, has remained challenging because of a limited ability to monitor oil body location and composition during germination in situ Here, we demonstrate via in situ, label-free imaging that oil bodies are highly dynamic intracellular organelles that are morphologically and biochemically remodelled extensively during germination. Label-free, coherent Raman microscopy (CRM) combined with bulk biochemical measurements revealed the temporal and spatial regulation of oil bodies in native soya bean cotyledons during the first eight days of germination. Oil bodies undergo a cycle of growth and shrinkage that is paralleled by lipid and protein compositional changes. Specifically, the total protein concentration associated with oil bodies increases in the first phase of germination and subsequently decreases. Lipids contained within the oil bodies change in saturation and chain length during germination. Our results show that CRM is a well-suited platform to monitor in situ lipid dynamics and local chemistry and that oil bodies are actively remodelled during germination. This underscores the dynamic role of lipid reservoirs in plant development. © 2016 The Authors.
Cross-check of ex-situ and in-situ metrology of a bendable temperature stabilized KB mirror
International Nuclear Information System (INIS)
Yuan Sheng; Goldberg, Kenneth A.; Yashchuk, Valeriy V.; Celestre, Richard; McKinney, Wayne R.; Morrison, Gregory; Macdougall, James; Mochi, Iacopo; Warwick, Tony
2011-01-01
At the Advanced Light Source (ALS), we are developing broadly applicable, high-accuracy, in-situ, at-wavelength wavefront slope measurement techniques for Kirkpatrick-Baez (KB) mirror nano-focusing. In this paper, we report an initial cross-check of ex-situ and in-situ metrology of a bendable temperature stabilized KB mirror. This cross-check provides a validation of the in-situ shearing interferometry, currently under development at the ALS.
Utilizing The Synergy of Airborne Backscatter Lidar and In-Situ Measurements for Evaluating CALIPSO
Directory of Open Access Journals (Sweden)
Tsekeri Alexandra
2016-01-01
Full Text Available Airborne campaigns dedicated to satellite validation are crucial for the effective global aerosol monitoring. CALIPSO is currently the only active remote sensing satellite mission, acquiring the vertical profiles of the aerosol backscatter and extinction coefficients. Here we present a method for CALIPSO evaluation from combining lidar and in-situ airborne measurements. The limitations of the method have to do mainly with the in-situ instrumentation capabilities and the hydration modelling. We also discuss the future implementation of our method in the ICE-D campaign (Cape Verde, August 2015.
Deasy, William; Shepherd, Tom; Alexander, Colin J; Birch, A Nicholas E; Evans, K Andrew
2016-11-01
Research on plant root chemical ecology has benefited greatly from recent developments in analytical chemistry. Numerous reports document techniques for sampling root volatiles, although only a limited number describe in situ collection. To demonstrate a new method for non-invasive in situ passive sampling using solid phase micro extraction (SPME), from the immediate vicinity of growing roots. SPME fibres inserted into polyfluorotetrafluoroethylene (PTFE) sampling tubes located in situ which were either perforated, covered with stainless steel mesh or with microporous PTFE tubing, were used for non-invasive sub-surface sampling of root volatiles from glasshouse-grown broccoli. Sampling methods were compared with above surface headspace collection using Tenax TA. The roots were either mechanically damaged or infested with Delia radicum larvae. Principal component analysis (PCA) was used to investigate the effect of damage on the composition of volatiles released by broccoli roots. Analyses by gas chromatography-mass spectrometry (GC-MS) with SPME and automated thermal desorption (ATD) confirmed that sulphur compounds, showing characteristic temporal emission patterns, were the principal volatiles released by roots following insect larval damage. Use of SPME with in situ perforated PTFE sampling tubes was the most robust method for out-of-lab sampling. This study describes a new method for non-invasive passive sampling of volatiles in situ from intact and insect damaged roots using SPME. The method is highly suitable for remote sampling and has potential for wide application in chemical ecology/root/soil research. Copyright © 2016 John Wiley & Sons, Ltd. Copyright © 2016 John Wiley & Sons, Ltd.
Zheng, Xiaoyu; Quan, Honglin; Li, Xiaoxin; He, Hai; Ye, Qinglan; Xu, Xuetang; Wang, Fan
2016-09-29
Three-dimensional (3D) hybrid nanostructured arrays grown on a flexible substrate have recently attracted great attention owing to their potential application as supercapacitor electrodes in portable and wearable electronic devices. Here, we report an in situ conversion of Ni-Co active electrode materials for the fabrication of high-performance electrodes. Ni-Co carbonate hydroxide nanowire arrays on carbon cloth were initially synthesized via a hydrothermal method, and they were gradually converted to Ni-Co (oxy)hydroxide nanowire-supported nanoflake arrays after soaking in an alkaline solution. The evolution of the supercapacitor performance of the soaked electrode was investigated in detail. The areal capacitance increases from 281 mF cm -2 at 1 mA cm -2 to 3710 and 3900 mF cm -2 after soaking for 36 h and 48 h, respectively. More interestingly, the electrode also shows an increased capacitance with charge/discharge cycles due to the long-time soaking in KOH solution, suggesting novel cycling durability. The enhancement in capacitive performance should be related to the formation of a unique nanowire-supported nanoflake array architecture, which controls the agglomeration of nanoflakes, making them fully activated. As a result, the facile in situ fabrication of the hybrid architectural design in this study provides a new approach to fabricate high-performance Ni/Co based hydroxide nanostructure arrays for next-generation energy storage devices.
In situ detection of warfarin using time-correlated single-photon counting
Energy Technology Data Exchange (ETDEWEB)
Rosengren, Annika M.; Karlsson, Bjoern C.G. [Bioorganic and Biophysical Chemistry Laboratory, School of Natural Sciences, Linnaeus University, SE-391 82 Kalmar (Sweden); Naeslund, Inga; Andersson, Per Ola [Swedish Defence Research Agency, FOI, CBRN Defence and Security, SE-901 82 Umea (Sweden); Nicholls, Ian A., E-mail: ian.a.nicholls@bioorg.uu.se [Bioorganic and Biophysical Chemistry Laboratory, School of Natural Sciences, Linnaeus University, SE-391 82 Kalmar (Sweden); Department of Biochemistry and Organic Chemistry Laboratory, Uppsala University, SE-751 23 Uppsala (Sweden)
2011-04-01
Highlights: {yields} Direct in situ measurement of specific isomeric forms of the anticoagulant warfarin. {yields} TCSPC spectroscopy in conjunction with synthetic Sudlow I binding site receptors. {yields} Development of sensor principle for use in clinical and environmental monitoring. -- Abstract: Here we report on a novel method for the direct in situ measurement of specific isomeric forms of the anticoagulant warfarin using time correlated single-photon counting (TCSPC) spectroscopy in conjunction with synthetic Sudlow I binding site receptors. The method is highly robust over the clinically significant concentration range, and demonstrates the potential of the binding site mimics in conjunction with the spectroscopic strategy employed here for the determination of this important pharmaceutical in clinical or even environmental samples.
In situ detection of warfarin using time-correlated single-photon counting
International Nuclear Information System (INIS)
Rosengren, Annika M.; Karlsson, Bjoern C.G.; Naeslund, Inga; Andersson, Per Ola; Nicholls, Ian A.
2011-01-01
Highlights: → Direct in situ measurement of specific isomeric forms of the anticoagulant warfarin. → TCSPC spectroscopy in conjunction with synthetic Sudlow I binding site receptors. → Development of sensor principle for use in clinical and environmental monitoring. -- Abstract: Here we report on a novel method for the direct in situ measurement of specific isomeric forms of the anticoagulant warfarin using time correlated single-photon counting (TCSPC) spectroscopy in conjunction with synthetic Sudlow I binding site receptors. The method is highly robust over the clinically significant concentration range, and demonstrates the potential of the binding site mimics in conjunction with the spectroscopic strategy employed here for the determination of this important pharmaceutical in clinical or even environmental samples.
In situ neutron depth profiling: A powerful method to probe lithium transport in micro-batteries
Oudenhoven, J.F.M.; Labohm, F.; Mulder, M.; Niessen, R.A.H.; Mulder, F.M.; Notten, P.H.L.
2011-01-01
In situ neutron depth profiling (NDP) offers the possibility to observe lithium transport inside micro-batteries during battery operation. It is demonstrated that NDP results are consistent with the results of electrochemical measurements, and that the use of an enriched6LiCoO2 cathode offers more
Pestman, B.J.; Hosson, J.Th.M. De
1992-01-01
The interaction between lattice dislocations and grain boundaries in Ni3Al has been investigated by means of in situ TEM deformation experiments. The interaction between screw dislocations and a coherent twin boundary could be analyzed in detail. The interaction mechanism found experimentally was
Wang, Long; Cai, Li; Qian, Hai; Lawton, Michael T; Shi, Xiang'en
2018-05-02
In situ side-to-side (STS) revascularization is an intracranial-intracranial (IC-IC) bypass technique that is increasingly used to treat complex aneurysms and cerebral ischemia. This sophisticated technique involves connecting two proximal parallel vessels to create an artificial conduit for blood flow. This study aims to provide a detailed description of the configuration of the STS bypass technique and extensive information regarding its technical characteristics, current anastomosis approaches and surgical significance. A literature search was performed using the PubMed, Medline, ScienceDirect, Embase, Wiley Online Library, Cambridge Journals, SAGE Journals, Oxford Journals, Research Gate, and Google Scholar databases. The terms "intracranial-intracranial bypass", "in situ bypass", "communicating bypass" and "STS anastomosis" were searched to identify pertinent articles. Articles involving in situ STS anastomosis combined with other bypass methods were excluded. Computer tablet-drawn illustrations of this technique are provided to enhance comprehension. In total, seventy articles that met our search and inclusion criteria were identified. Overall, the radiographical and clinical outcomes of one-hundred and thirty-two (125 aneurysm and 7 cerebral ischemia) patients who underwent in situ STS revascularization were analyzed. IC-IC bypass in the STS fashion can be a safe and effective strategy for the management of complex intracranial aneurysms and cerebral ischemia and is particularly attractive in rescue, anticipated and troubleshooting cases. Despite its extreme rarity, a de novo aneurysm may be observed following STS anastomosis; thus, long-term follow-up is mandatory. Vascular neurosurgeons should consider including this procedure in their treatment armamentarium. Copyright © 2018. Published by Elsevier Inc.
In Situ Field Testing of Processes
International Nuclear Information System (INIS)
Wang, J.
2001-01-01
The purpose of this Analysis/Model Report (AMR) is to update and document the data and subsequent analyses from ambient field-testing activities performed in underground drifts of the Yucca Mountain Site Characterization Project (YMP). This revision updates data and analyses presented in the initial issue of this AMR. This AMR was developed in accordance with the ''Technical Work Plan for Unsaturated Zone (UZ) Flow and Transport Process Model Report'' and ''Technical Work Plan for UZ Flow, Transport, and Coupled Processes Process Model Report. These activities were performed to investigate in situ flow and transport processes. The evaluations provide the necessary framework to: (1) refine and confirm the conceptual model of matrix and fracture processes in the unsaturated zone (UZ) and (2) analyze the impact of excavation (including use of construction water and effect of ventilation) on the UZ flow and transport processes. This AMR is intended to support revisions to ''Conceptual and Numerical Models for UZ Flow and Transport'' and ''Unsaturated Zone Flow and Transport Model Process Model Report''. In general, the results discussed in this AMR are from studies conducted using a combination or a subset of the following three approaches: (1) air-injection tests, (2) liquid-release tests, and (3) moisture monitoring using in-drift sensors or in-borehole sensors, to evaluate the impact of excavation, ventilation, and construction-water usage on the surrounding rocks. The liquid-release tests and air-injection tests provide an evaluation of in situ fracture flow and the competing processes of matrix imbibition. Only the findings from testing and data not covered in the ''Seepage Calibration Model and Seepage Testing Data'' are analyzed in detail in the AMR
International Nuclear Information System (INIS)
Li Qiaoling; Zhang Cunrui; Li Jianqiang
2011-01-01
Research highlights: → Polypyrrole/Fe-doped TiO 2 composite is prepared by in situ polymerization of pyrrole on the Fe-doped TiO 2 template. → The Fe-doped TiO 2 microbelts are prepared by sol-gel method using the absorbent cotton template for the first time. → Then the Fe-doped TiO 2 microbelts are used as template for the preparation of polypyrrole/Fe-doped TiO 2 composites. → The structure, morphology and properties of the composites are characterized with scanning electron microscope (SEM), IR, Net-work Analyzer. → A possible formation mechanism of Fe-doped TiO 2 microbelts and polypyrrole/Fe-doped TiO 2 composites has been proposed. → The effect of the mol ratio of pyrrole/Fe-doped TiO 2 on the photocatalysis properties and microwave loss properties of the composites is investigated. - Abstract: The Fe-doped TiO 2 microbelts were prepared by sol-gel method using the absorbent cotton template for the first time. Then the Fe-doped TiO 2 microbelts were used as templates for the preparation of polypyrrole/Fe-doped TiO 2 composites. Polypyrrole/Fe-doped TiO 2 composites were prepared by in situ polymerization of pyrrole on the Fe-doped TiO 2 template. The structure, morphology and properties of the composites were characterized with scanning electron microscope (SEM), FTIR, Net-work Analyzer. The possible formation mechanisms of Fe-doped TiO 2 microbelts and polypyrrole/Fe-doped TiO 2 composites have been proposed. The effect of the molar ratio of pyrrole/Fe-doped TiO 2 on the photocatalytic properties and microwave loss properties of the composites was investigated.
A new in situ model to study erosive enamel wear, a clinical pilot study.
Ruben, J.L.; Truin, G.J.; Bronkhorst, E.M.; Huysmans, M.C.D.N.J.M.
2017-01-01
OBJECTIVES: To develop an in situ model for erosive wear research which allows for more clinically relevant exposure parameters than other in situ models and to show tooth site-specific erosive wear effect of an acid challenge of orange juice on enamel. METHODS: This pilot study included 6
In Situ/Remote Sensing Integration to Assess Forest Health—A Review
Directory of Open Access Journals (Sweden)
Marion Pause
2016-06-01
Full Text Available For mapping, quantifying and monitoring regional and global forest health, satellite remote sensing provides fundamental data for the observation of spatial and temporal forest patterns and processes. While new remote-sensing technologies are able to detect forest data in high quality and large quantity, operational applications are still limited by deficits of in situ verification. In situ sampling data as input is required in order to add value to physical imaging remote sensing observations and possibilities to interlink the forest health assessment with biotic and abiotic factors. Numerous methods on how to link remote sensing and in situ data have been presented in the scientific literature using e.g. empirical and physical-based models. In situ data differs in type, quality and quantity between case studies. The irregular subsets of in situ data availability limit the exploitation of available satellite remote sensing data. To achieve a broad implementation of satellite remote sensing data in forest monitoring and management, a standardization of in situ data, workflows and products is essential and necessary for user acceptance. The key focus of the review is a discussion of concept and is designed to bridge gaps of understanding between forestry and remote sensing science community. Methodological approaches for in situ/remote-sensing implementation are organized and evaluated with respect to qualifying for forest monitoring. Research gaps and recommendations for standardization of remote-sensing based products are discussed. Concluding the importance of outstanding organizational work to provide a legally accepted framework for new information products in forestry are highlighted.
In situ Biofilm Quantification in Bioelectrochemical Systems by using Optical Coherence Tomography.
Molenaar, Sam D; Sleutels, Tom; Pereira, Joao; Iorio, Matteo; Borsje, Casper; Zamudio, Julian A; Fabregat-Santiago, Francisco; Buisman, Cees J N; Ter Heijne, Annemiek
2018-04-25
Detailed studies of microbial growth in bioelectrochemical systems (BESs) are required for their suitable design and operation. Here, we report the use of optical coherence tomography (OCT) as a tool for in situ and noninvasive quantification of biofilm growth on electrodes (bioanodes). An experimental platform is designed and described in which transparent electrodes are used to allow real-time, 3D biofilm imaging. The accuracy and precision of the developed method is assessed by relating the OCT results to well-established standards for biofilm quantification (chemical oxygen demand (COD) and total N content) and show high correspondence to these standards. Biofilm thickness observed by OCT ranged between 3 and 90 μm for experimental durations ranging from 1 to 24 days. This translated to growth yields between 38 and 42 mgCODbiomass gCODacetate -1 at an anode potential of -0.35 V versus Ag/AgCl. Time-lapse observations of an experimental run performed in duplicate show high reproducibility in obtained microbial growth yield by the developed method. As such, we identify OCT as a powerful tool for conducting in-depth characterizations of microbial growth dynamics in BESs. Additionally, the presented platform allows concomitant application of this method with various optical and electrochemical techniques. © 2018 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA.
International Nuclear Information System (INIS)
Huckaby, J.L.; Edwards, J.A.; Evans, J.C.
1996-08-01
This report discusses comparison tests for two methods of collecting vapor samples from the Hanford Site high-level radioactive waste tank headspaces. The two sampling methods compared are the truck-mounted vapor sampling system (VSS) and the cart-mounted in-situ vapor sampling (ISVS). Three tanks were sampled by both the VSS and ISVS methods from the same access risers within the same 8-hour period. These tanks have diverse headspace compositions and they represent the highest known level of several key vapor analytes
First use of in situ vitrification on radioactive wastes
International Nuclear Information System (INIS)
Bowlds, L.
1992-01-01
A high-temperature method for containing hazardous wastes, which was first developed in the 1980s, is being adapted for the in situ treatment of buried radioactive wastes by the US DOE's Idaho National Engineering Laboratory (INEL), following its recent report on successful preliminary tests. The method, called in situ vitrification (ISV), is an electrically induced thermal process that melts and fuses soil and wastes into a glass-like material at least as strong as natural obsidian or granite. Gases released during the process are captured and treated by an off-gas treatment system. After the wastes are vitrified, they could be left in place, or the mass could be broken up and transported to a disposal site. The glass-like substance would be chemically and physically similar to obsidian and from 4 to 10 times more durable than typical borosilicate glasses used to immobolize high-level nuclear wastes
Customised search and comparison of in situ, satellite and model data for ocean modellers
Hamre, Torill; Vines, Aleksander; Lygre, Kjetil
2014-05-01
was reflected in the web portal's graphical user interface, where the groups and subgroups were organized in a tree structure, enabling the modeller to quickly get an overview of available data, going into more detail (subgroups) if needed or staying at a higher level of abstraction (merging the parameters below) if this provided a better base for comparison with the model parameters. Once a suitable level of detail, as determined by the modeller, was decided, the system would retrieve available in situ parameters. The modellers could then select among the pre-defined models or upload his own model forecast file (in NetCDF/CF format), for comparison with the retrieved in situ data. The comparison can be shown in different kinds of plots (e.g. scatter plots), through simple statistical measures or near-coincident values of in situ of model points can be exported for further analysis in the modeller's own tools. During data search and presentation, the modeller can determine both query criteria and what associated metadata to include in the display and export of the retrieved data. Satellite-derived parameters can be queried and compared with model results in the same manner. With the developed prototype system, we have demonstrated that a customised tool for searching, presenting, comparing and exporting ocean data from multiple platforms (in situ, satellite, model), makes it easy to compare model results with independent observations. With further enhancement of functionality and inclusion of more data, we believe the resulting system can greatly benefit the wider community of ocean modellers looking for data and tools to validate their models.
Kamoun, M; Ammar, H; Théwis, A; Beckers, Y; France, J; López, S
2014-11-01
The use of stable (15)N as a marker to determine microbial contamination in nylon bag incubation residues to estimate protein degradability was investigated. Three methods using (15)N were compared: (15)N-labeled forage (dilution method, LF), (15)N enrichment of rumen solids-associated bacteria (SAB), and (15)N enrichment of rumen liquid-associated bacteria (LAB). Herbage from forages differing in protein and fiber contents (early-cut Italian ryegrass, late-cut Italian ryegrass, and red clover) were freeze-dried and ground and then incubated in situ in the rumen of 3 steers for 3, 6, 12, 24, and 48 h using the nylon bag technique. The (15)N-labeled forages were obtained by fertilizing the plots where herbage was grown with (15)NH4 (15)NO3. Unlabeled forages (obtained from plots fertilized with NH4NO3) were incubated at the same time that ((15)NH4)2SO4 was continuously infused into the rumen of the steers, and then pellets of labeled SAB and LAB were isolated by differential centrifugation of samples of ruminal contents. The proportion of bacterial N in the incubation residues increased from 0.09 and 0.45 g bacterial N/g total N at 3 h of incubation to 0.37 and 0.85 g bacterial N/g total N at 48 h of incubation for early-cut and late-cut ryegrass, respectively. There were differences (P forage (late-cut ryegrass) was 0.51, whereas the corrected values were 0.85, 0.84, and 0.77 for the LF, SAB, and LAB methods, respectively. With early-cut ryegrass and red clover, the differences between uncorrected and corrected values ranged between 6% and 13%, with small differences among the labeling methods. Generally, methods using labeled forage or labeled SAB and LAB provided similar corrected degradability values. The accuracy in estimating the extent of degradation of protein in the rumen from in situ disappearance curves is improved when values are corrected for microbial contamination of the bag residue.
A new FSA approach for in situ γ ray spectroscopy
International Nuclear Information System (INIS)
Caciolli, A.; Baldoncini, M.; Bezzon, G.P.; Broggini, C.; Buso, G.P.; Callegari, I.; Colonna, T.; Fiorentini, G.; Guastaldi, E.; Mantovani, F.; Massa, G.; Menegazzo, R.; Mou, L.; Alvarez, C. Rossi
2012-01-01
An increasing demand of environmental radioactivity monitoring comes both from the scientific community and from the society. This requires accurate, reliable and fast response preferably from portable radiation detectors. Thanks to recent improvements in the technology, γ spectroscopy with sodium iodide scintillators has been proved to be an excellent tool for in-situ measurements for the identification and quantitative determination of γ ray emitting radioisotopes, reducing time and costs. Both for geological and civil purposes not only 40 K, 238 U, and 232 Th have to be measured, but there is also a growing interest to determine the abundances of anthropic elements, like 137 Cs and 131 I, which are used to monitor the effect of nuclear accidents or other human activities. The Full Spectrum Analysis (FSA) approach has been chosen to analyze the γ spectra. The Non Negative Least Square (NNLS) and the energy calibration adjustment have been implemented in this method for the first time in order to correct the intrinsic problem related with the χ 2 minimization which could lead to artifacts and non physical results in the analysis. A new calibration procedure has been developed for the FSA method by using in situ γ spectra instead of calibration pad spectra. Finally, the new method has been validated by acquiring γ spectra with a 10.16 cm × 10.16 cm sodium iodide detector in 80 different sites in the Ombrone basin, in Tuscany. The results from the FSA method have been compared with the laboratory measurements by using HPGe detectors on soil samples collected particular, the 137 Cs isotopes has been implemented in the analysis since it has been found not negligible during the in-situ measurements. - Highlights: ► The Full Spectrum Analysis technique is investigated with sodium iodide scintillator. ► Artifacts due to the free chi 2 minimization in standard FSA are solved applying the NNLS. ► New calibration approach has been developed without using
DEFF Research Database (Denmark)
Duan, Zhi; Brüggemann, Dagmar Adeline; Siegumfeldt, Henrik
2009-01-01
three small synthetic peptides of the alpha(s1)-casein sequence. These peptides traverse enzymatic cleavage sites of casein during cheese ripening. The specificity of the generated anti-peptide antibodies was determined by ELISA and Western blot. Finally, an immunofluorescent labeling protocol......A novel method to monitor in situ hydrolyzable casein fragments during cheese ripening by using immunofluorescent labeling and confocal laser scanning microscopy (CLSM) was developed. Monoclonal single chain variable fragments of antibody (scFvs) were generated by antibody phage display toward...
PERFORMANCE CONFIRMATION IN-SITU INSTRUMENTATION
International Nuclear Information System (INIS)
N.T. Raczka
2000-01-01
The purpose of this document is to identify and analyze the types of in-situ instruments and methods that could be used in support of the data acquisition portion of the Performance Confirmation (PC) program at the potential nuclear waste repository at Yucca Mountain. The PC program will require geomechanical , geophysical, thermal, and hydrologic instrumentation of several kinds. This analysis is being prepared to document the technical issues associated with each type of measurement during the PC period. This analysis utilizes the ''Performance Confirmation Input Criteria'' (CRWMS M andO 1999a) as its starting point. The scope of this analysis is primarily on the period after the start of waste package emplacement and before permanent closure of the repository, a period lasting between 15 and 300 years after last package emplacement (Stroupe 2000, Attachment 1, p. 1). The primary objectives of this analysis are to: (1) Review the design criteria as presented in the ''Performance Confirmation Input Criteria'' (CRWMS M andO 1999a). The scope of this analysis will be limited to the instrumentation related to parameters that require continuous monitoring of the conditions underground. (2) Preliminary identification and listing of the data requirements and parameters as related to the current repository layout in support of PC monitoring. (3) Preliminary identification of methods and instrumentation for the acquisition of the required data. Although the ''Performance Confirmation Input Criteria'' (CRWMS M andO 1999a) defines a broad range of data that must be obtained from a variety of methods, the focus of this analysis is on instrumentation related to the performance of the rock mass and the formation of water in the repository environment, that is obtainable from in-situ observation, testing, and monitoring
International Nuclear Information System (INIS)
Joshi, Leela; Singh, Arun Kumar; Prakash, Rajiv
2012-01-01
Composites of polyindole (PIn), a conducting polymer, with carboxylated-multiwalled carbon nanotubes (c-MWCNT/PIn) were synthesized; the synthesis was done using (i) two miscible solvents (in-situ method) and (ii) two immiscible solvents (interfacial method). A tubular composite, with a uniform coating of the polymer over c-MWCNTs, was observed in the case of interfacial synthesis. However, the in-situ synthesis of c-MWCNT/PIn composites exhibited a densely packed spherical morphology, with c-MWCNT incorporated within the polymer spheres. The spherical morphology was probably obtained due to fast polymerization kinetics and the formation of micelles in case of in-situ polymerization, whereas tubular morphology was obtained in case of interfacial polymerization due to the sufficient time provided for the growth of polymer chains over the c-MWCNT surfaces. Nanoscale electrical properties of composites, in a metal/(c-MWCNT/PIn) configuration, were studied using current sensing atomic force microscopy. Interfacial c-MWCNT/PIn composite, on Al metal substrate, exhibited a typical rectifying diode behavior. This composite had manifested enormous potential for electronic applications and fabrication of nanoscale organic devices. Highlights: ► Polyindole/c-MWNT nanocomposites produced by in-situ and interfacial polymerization. ► Densely packed spherical morphology was observed in in-situ polymerization route. ► Tubular core-shell morphology was observed in interfacial polymerization route. ► Interfacial nanocomposite manifested a nano-schottky junction with Al metal.
In-situ burn operational procedures development exercises
International Nuclear Information System (INIS)
Bitting, K.; Gynther, J.; Drieu, M.; Tidemann, A.; Martin, R.
2001-01-01
The United States Coast Guard, the Texas General Land Office and the National Response Corporation conducted three at-sea oil spill exercises in 1999 and 2000 to test and evaluate a variety of methods to perform in-situ burning (ISB) operations at sea. ISB is seldom used during actual responses, particularly in offshore environments because there is no detailed ISB operation plan for specific regional response teams. There is also a lack of sufficient ISB resources, both equipment and trained personnel, that can be mobilized within the limited ISB window-of-opportunity. There is also a misconception regarding the costs and benefits of ISB. For these exercises, the oil slick was simulated with several tons of oranges. The primary objective was to examine the safe, effective and efficient implementation of ISB. The exercises involved the use of actual response vessels, water-cooled fire booms, helicopters and helitorches. Specific manoeuvres were conducted and particular activities were measured. The experimental data was recorded for future use as a planning and training tool. The exercises demonstrated that ISB is a viable and efficient response tool if it is used in the right situation. The biggest short fall of these exercises was the inability to burn real oil on the water (the oranges did not provide enough data). It was concluded that ISB is not always the best tool for every situation. Dispersants and mechanical recovery are also viable options. 1 tab., 6 figs
Integrating In-Situ and Ex-Situ Data Management Processes for Biodiversity Conservation
Directory of Open Access Journals (Sweden)
Karin R. Schwartz
2017-10-01
Full Text Available There is an increasing need for a “one plan approach” for conservation strategies that integrate in-situ and ex-situ management processes. Zoological institutions contribute directly to threatened species conservation through paradigms, such as reintroduction, head-starting, supplementation, or rescue/rehabilitation/release. This in-situ/ex-situ integration necessitates collaboration at all levels of conservation action including planning, implementation, monitoring and assessment to drive adaptive management processes. Each component is dependent on the availability and accuracy of data for evidence to facilitate evaluation and adaptive management processes. The Zoological Information Management System (ZIMS, managed by Species360, is a centralized web-based information system used in zoological institutions worldwide to pool life history, behavior and health data and facilitate animal husbandry, health, and breeding management processes. Currently used for few integrated conservation programs, ZIMS is an innovative tool that offers a new opportunity to link data management processes for animals that spend a part of their lives under human care and part in their natural environment and has great potential for use in managed wild populations.
Phosphate interference during in situ treatment for arsenic in groundwater.
Brunsting, Joseph H; McBean, Edward A
2014-01-01
Contamination of groundwater by arsenic is a problem in many areas of the world, particularly in West Bengal (India) and Bangladesh, where reducing conditions in groundwater are the cause. In situ treatment is a novel approach wherein, by introduction of dissolved oxygen (DO), advantages over other treatment methods can be achieved through simplicity, not using chemicals, and not requiring disposal of arsenic-rich wastes. A lab-scale test of in situ treatment by air sparging, using a solution with approximately 5.3 mg L(-1) ferrous iron and 200 μg L(-1) arsenate, showed removal of arsenate in the range of 59%. A significant obstacle exists, however, due to the interference of phosphate since phosphate competes for adsorption sites on oxidized iron precipitates. A lab-scale test including 0.5 mg L(-1) phosphate showed negligible removal of arsenate. In situ treatment by air sparging demonstrates considerable promise for removal of arsenic from groundwater where iron is present in considerable quantities and phosphates are low.
Fluorescence in situ hybridization: an improved method of quantitating chromosome damage and repair
International Nuclear Information System (INIS)
Brown, J.M.; Evans, J.W.
1993-01-01
The authors combined fluorescence in situ hybridization (FISH) with specific full-length chromosome probes using the premature chromosome condensation (PCC) technique chromosome condensation (PCC) technique to simplify scoring chromosome damage and its repair. They have shown the technique works well and enables breaks and exchanges to be readily detected and scored in individual chromosomes. A chromosome 4 full-length specific library has been used in initial studies. (UK)
EM-54 Technology Development In Situ Remediation Integrated Program
International Nuclear Information System (INIS)
1993-08-01
The Department of Energy (DOE) established the Office of Technology Development (EM-50) as an element of Environmental Restoration and Waste Management (EM) in November 1989. EM manages remediation of all DOE sites as well as wastes from current operations. The goal of the EM program is to minimize risks to human health, safety and the environment, and to bring all DOE sites into compliance with Federal, state, and local regulations by 2019. EM-50 is charged with developing new technologies that are safer, more effective and less expensive than current methods. The In Situ Remediation Integrated Program (the subject of this report) is part of EM-541, the Environmental Restoration Research and Development Division of EM-54. The In Situ Remediation Integrated Program (ISR IP) was instituted out of recognition that in situ remediation could fulfill three important criteria: Significant cost reduction of cleanup by eliminating or minimizing excavation, transportation, and disposal of wastes; reduced health impacts on workers and the public by minimizing exposure to wastes during excavation and processing; and remediation of inaccessible sites, including: deep subsurfaces; in, under, and around buildings. Buried waste, contaminated soils and groundwater, and containerized wastes are all candidates for in situ remediation. Contaminants include radioactive wastes, volatile and non-volatile organics, heavy metals, nitrates, and explosive materials. The ISR IP tends to facilitate development of in situ remediation technologies for hazardous, radioactive, and mixed wastes in soils, groundwater, and storage tanks. Near-term focus is on containment of the wastes, with treatment receiving greater effort in future years
Malicka-Durczak, Anna; Korski, Konstanty; Ibbs, Matthew
2012-01-01
This project compared HER-2/neu gene status in breast cancers, as demonstrated by FISH (fluorescent in situ hybridization) and CISH (chromogenic in situ hybridization) and using a tissue microarray (TMA). The study also aimed to show whether the TMA technique could be used in clinical diagnostics, rather than remain a scientific tool. A TMA was constructed using 121 breast cancer specimens, 6 cores from each specimen. Demonstration and assessment of HER-2/neu gene status was by FISH (Vysis Path) and CISH (DAKO Duo CISH). The 121 breast cancer specimens were divided into 3 groups by HER-2 status, as determined by immunohistochemistry. In the HER-2 negative group no amplification was observed in 36 out of 40 cases. 3 cases showed amplification by both methods and one by CISH alone. The equivocal HER-2 group showed no amplification in 30 out of 41 cases and amplification in 9 cases. One case was FISH negative CISH positive and one was discarded. In the HER-2 positive group, amplification was confirmed in 37 of the 40 cases by both methods. 3 cases were unsuitable for assessment. This study indicated that CISH is a sensitive alternative to FISH in detecting HER2 gene amplification and may replace FISH in HER2 testing. Good agreement was observed between methods (98.5% - 119 out of 121 cases). Furthermore, as only 4 out of 121 cases were unsuitable for assessment (no signal or missing TMA cores) - it may be feasible to use TMA in diagnostics.
In situ coating multiwalled carbon nanotubes with CdS nanoparticles
International Nuclear Information System (INIS)
Liu Yangqiao; Gao Lian
2005-01-01
CdS nanoparticles were homogeneously coated on multiwalled carbon nanotubes by an in situ method through introducing thiol groups onto the tube wall using a novel method. A cationic polyelectrolyte containing reactive imine groups, polyethyleneimine (PEI), was firstly adsorbed on the surface of nanotubes. 3-Mercaptopropionic acid (MPA) was then anchored by an amidation reaction between its carboxyl group and the imine group of the polyelectrolyte under the activation of carbodiimide reagents. These -SH terminated MWCNTs were coated with CdS nanoparticles by an in situ method. The phase composition, microstructure, and the UV-vis properties of the CdS coated MWCNTs were characterized. The addition of the carbodiimide reagents played an important role in linking the MPA with PEI covalently and subsequently coating the MWCNTs with CdS homogeneously. A blue shift in the absorption edge was observed for the MWCNTs-CdS hybrid material due to the quantum size effect
Innovative technologies for in-situ remediation
International Nuclear Information System (INIS)
Ragaini, R.; Aines, R.; Knapp, R.; Matthews, S.; Yow, J.
1994-06-01
LLNL is developing several innovative remediation technologies as long-term improvements to the current pump and treat approaches to cleaning up contaminated soils and groundwater. These technologies include dynamic underground stripping, in-situ microbial filters, and remediation using bremsstrahlung radiation. Concentrated underground organic contaminant plumes are one of the most prevalent groundwater contamination sources. The solvent or fuel can percolate deep into the earth, often into water-bearing regions. Collecting as a separate, liquid organic phase called dense non-aqueous-phase liquids (DNAPLs), or light NAPLs (LNAPLs), these contaminants provide a source term that continuously compromises surrounding groundwater. This type of spill is one of the most difficult environmental problems to remediate. Attempts to remove such material requires a huge amount of water which must be washed through the system to clean it, requiring decades. Traditional pump and treat approaches have not been successful. LLNL has developed several innovative technologies to clean up NAPL contamination. Detailed descriptions of these technologies are given
In-situ measurements of soil-water conductivity
International Nuclear Information System (INIS)
Murphy, C.E.
1978-01-01
Radionuclides and other environmentally important materials often move in association with water. In terrestrial ecosystems, the storage and movement of water in the soil is of prime importance to the hydrologic cycle of the ecosystem. The soil-water conductivity (the rate at which water moves through the soil) is a necessary input to models of soil-water movement. In situ techniques for measurement of soil-water conductivity have the advantage of averaging soil-water properties over larger areas than most laboratory methods. The in situ techniques also cause minimum disturbance of the soil under investigation. Results of measurements using a period of soil-water drainage after initial wetting indicate that soil-water conductivity and its variation with soil-water content can be determined with reasonable accuracy for the plot where the measurements were made. Further investigations are being carried out to look at variability between plots within a soil type
In-Situ Visualization Experiments with ParaView Cinema in RAGE
Energy Technology Data Exchange (ETDEWEB)
Kares, Robert John [Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
2015-10-15
A previous paper described some numerical experiments performed using the ParaView/Catalyst in-situ visualization infrastructure deployed in the Los Alamos RAGE radiation-hydrodynamics code to produce images from a running large scale 3D ICF simulation. One challenge of the in-situ approach apparent in these experiments was the difficulty of choosing parameters likes isosurface values for the visualizations to be produced from the running simulation without the benefit of prior knowledge of the simulation results and the resultant cost of recomputing in-situ generated images when parameters are chosen suboptimally. A proposed method of addressing this difficulty is to simply render multiple images at runtime with a range of possible parameter values to produce a large database of images and to provide the user with a tool for managing the resulting database of imagery. Recently, ParaView/Catalyst has been extended to include such a capability via the so-called Cinema framework. Here I describe some initial experiments with the first delivery of Cinema and make some recommendations for future extensions of Cinema’s capabilities.
Energy Technology Data Exchange (ETDEWEB)
Bergman, H.L.
1980-01-04
This is the third annual progress report for a continuing EPA-DOE jointly funded project to evaluate the effects of aqueous effluents from in situ fossil-fuel processing technologies on aquatic biota. The project is organized into four project tasks: (1) literature review; (2) process water screening; (3) methods development; and (4) recommendations. Our Bibliography of aquatic ecosystem effects, analytical methods and treatment technologies for organic compounds in advanced fossil-fuel processing effluents was submitted to the EPA for publication. The bibliography contains 1314 citations indexed by chemicals, keywords, taxa and authors. We estimate that the second bibliography volume will contain approximately 1500 citations and be completed in February. We compiled results from several laboratories of inorganic characterizations of 19 process waters: 55 simulated in situ oil-shale retort waters; and Hanna-3, Hanna-4B 01W and Lawrence Livermore Hoe Creek underground coal gasification condenser waters. These process waters were then compared to a published summary of the analyses from 18 simulated in situ oil-shale retort waters. We completed this year 96-h flow-through toxicity bioassays with fathead minnows and rainbow trout and 48-h flow-through bioassays with Daphnia pulicaria exposed to 5 oil-shale process waters, 1 tar-sand process water, 2 underground coal gasification condenser waters, 1 post-gasification backflood condenser water, as well as 2 bioassays with fossil-fuel process water constituents. The LC/sub 50/ toxicity values for these respective species when exposed to these waters are given in detail. (LTN)
Small-scale in-situ burn tests to develop operational proficiencies
International Nuclear Information System (INIS)
McCarthy, M.W.
1996-01-01
A small-scale hands-on in-situ burning experiment was conducted to prepare members of a response community in the event of an actual full scale in-situ burn. Two different styles of fire booms were deployed in open water and exposed to multiple test tank burns. Residual burned crude oil was recovered and the booms decontaminated. The experiments showed that all the methods used to gauge the depth of oil contained in the booms created an element of doubt in their accuracy. The main lessons learned pertained to pre-combustion volume estimation, oil slick ignition, and residue recovery. It was concluded that in-situ burning was a potential technique in oil spill response, but some refinement is still needed to be done with the oil retention booms. The operational costs associated with the experiment were minimal, given the nature of the project. 1 tab
In situ leach method for recovering uranium and related values
International Nuclear Information System (INIS)
Yan, T.Y.
1981-01-01
A process is provided for in-situ leaching of uranium from a calcium-containing clay which does not result in contamination of the clay formation by any cations not already present. A lixiviant is prepared by dissolving carbon dioxide into water having essentially the same cationic composition as that of the formation connate water. The solution is injected along with an oxidant, for example oxygen, into the formation. Calcium that has become dissolved in the lixiviant must be removed to control the pH, preferably by the addition of lime in a calcium precipitator. After calcium removal the lixiviant is filtered to remove suspended solids and is passed through an ion exchange resin or other uranium extraction means. The barren solution goes to a mix tank where carbon dioxide is added, and the fresh lixiviant is injected along with additional oxidant into the formation
CoMA, an experiment for the detailed in-situ analysis of collected cometary particulates
International Nuclear Information System (INIS)
Kissel, J.; Fechtig, H.; Jessberger, E.K.; Krueger, F.R.; Niemczyk, N.; Schaefer, G.; Zscheeg, H.
1989-01-01
After NASA accepted for the implementation phase (up to October 1990) our proposal for the CoMA investigation to be flown onboard CRAF to rendezvous with a comet, funding by the BMFT for instrument pre-development has been obtained. Most contracts could be let, some, however, with substantial delay due to administrative problems. Progress can be reported as the group in Bremen could demonstrate a mass resolution of 60 000 for a low magnetic field ICR-experiment, resolving 12 CH + and 13 C + ions. Also a first successful attempt was made to theoretically describe the dust collection efficiencies of different materials. As yet such a model did not exist - a fact that made the interpretation and extrapolation of laboratory data obsolete. The experimental setup in which the Indium liquid ion source is tested could be completed by the successful addition of a bunching structure. In future it will serve those partners that develop in detail the primary beam system. (orig.)
Cassette-based in-situ TEM sample inspection in the dual-beam FIB
International Nuclear Information System (INIS)
Kendrick, A B; Moore, T M; Zaykova-Feldman, L; Amador, G; Hammer, M
2008-01-01
A novel method is presented, combining site-specific TEM sample preparation and in-situ STEM analysis in a dual-beam microscope (FIB/SEM) fitted with a chamber mounted nano-manipulator. TEM samples are prepared using a modified in-situ, lift-out method, whereby the samples are thinned and oriented for immediate in-situ STEM analysis using the tilt, translation, and rotation capabilities of a FIB/SEM sample stage, a nano-manipulator, and a novel cassette. This cassette can provide a second tilt axis, orthogonal to the stage tilt axis, so that the STEM image contrast can be optimized to reveal the structural features of the sample (true STEM imaging in the FIB/SEM). The angles necessary for stage rotation and probe shaft rotation are calculated based on the position of the nano-manipulator relative to the stage and door and the stage tilt angle. A FIB/SEM instrument, equipped with a high resolution scanning electron column, can provide sufficiently high image resolution to enable many failure analysis and process control applications to be successfully carried out without requiring the use of a separate dedicated TEM/STEM instrument. The benefits of this novel approach are increased throughput and reduced cost per sample. Comparative analysis of different sample preparation methods is provided, and the STEM images obtained are shown.
Detection of hepatitis B virus DNA sequences in infected hepatocytes by in situ cytohybridisation
International Nuclear Information System (INIS)
Gowans, E.J.; Burrell, C.J.; Jilbert, A.R.; Marmion, B.P.
1981-01-01
Plasmid pHBV 114 DNA, which contains 73% of the genome of hepatitis B virus (HBV), was radiolabelled with tritium to 1-2 X 10(8) dpm/microgram by nick translation and used as a radioactive probe to detect HBV DNA present in sections of infected liver tissue by in situ hybridisation followed by autoradiography. Factors affecting the sensitivity of the reaction were examined, including different methods of fixation, hybridisation time, temperature, and buffers. The specificity of the reaction for detecting viral DNA was carefully established by the use of unrelated DNA probes, pretreatment of sections with DNAase, and comparing the stability of the binding of DNA probe at different temperatures, with the melting curve of double-stranded DNA in solution. In the one liver studied in detail, cells containing large amounts of viral DNA were distributed in foci corresponding to areas containing morphologically damaged hepatocytes. This observation suggested a relationship between active viral replication and cell damage. Viral DNA was found mainly in the cytoplasm, although a minority of nuclei in these foci were also positive
In Situ Hybridization Pada Kanker Payudara
Diah Witari, Ni Putu
2014-01-01
Kesulitan yang dijumpai pada penanganan kanker payudara adalah terjadinya kekambuhan atau relaps. Deteksi status HER2 pada pasien merupakan salah satu upaya untuk mendeteksi terjadinya relaps dan juga untuk menentukan jenis terapi yang ada diberikan. Ekspresi protein HER2 dapat dideteksi dengan immunohistochemistry (IHC), sedangkan mutasi gen HER2 dapat dideteksi dengan teknik in situ hybridization baik berupa fluorescence in situ hybridization (FISH) ataupun chromogenic in situ hy...
In Situ Techniques for Life Detection on Mars
Becker, L.; Brinckerhoff, W.; Cotter, R.
2006-12-01
The search for organic matter on Mars is rapidly emerging as a result of technological advancements and the study of early "life" on our own planet. As we learned from the Viking missions and the examination of martian meteorites, the criteria for establishing life require the appropriate strategy. One such approach would require careful mapping of the surface from orbit for the selection of the appropriate landing sites, robotic space missions equipped with several life detection in situ techniques for selection of samples, and sample return missions for additional verification of in situ results and laboratory measurements. It may, however, be possible to obtain critical information about the organic matter and associated mineral assemblages present on Mars, in situ, in a single measurement that is both capable of flight and is nondestructive to the sample. We discuss a new multi-source mass spectrometer, `MOMA' (Mars Organic Molecule Analyzer) that incorporates multiple methods of volatilizing and ionizing chemical compounds from intact samples without further processing or manipulation. Moreover, MOMA is capable of detecting a broad range of organics enabling the evaluation of the origin of the organics and the presence of terrestrial contaminants.
Baghdady, Yehia Z; Schug, Kevin A
2016-01-01
Accurate and specific analysis of target molecules in complex biological matrices remains a significant challenge, especially when ultra-trace detection limits are required. Liquid chromatography with mass spectrometry is often the method of choice for bioanalysis. Conventional sample preparation and clean-up methods prior to the analysis of biological fluids such as liquid-liquid extraction, solid-phase extraction, or protein precipitation are time-consuming, tedious, and can negatively affect target recovery and detection sensitivity. An alternative or complementary strategy is the use of an off-line or on-line in situ derivatization technique. In situ derivatization can be incorporated to directly derivatize target analytes in their native biological matrices, without any prior sample clean-up methods, to substitute or even enhance the extraction and preconcentration efficiency of these traditional sample preparation methods. Designed appropriately, it can reduce the number of sample preparation steps necessary prior to analysis. Moreover, in situ derivatization can be used to enhance the performance of the developed liquid chromatography with mass spectrometry-based bioanalysis methods regarding stability, chromatographic separation, selectivity, and ionization efficiency. This review presents an overview of the commonly used in situ derivatization techniques coupled to liquid chromatography with mass spectrometry-based bioanalysis to guide and to stimulate future research. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Detection of amide I signals of interfacial proteins in situ using SFG.
Wang, Jie; Even, Mark A; Chen, Xiaoyun; Schmaier, Alvin H; Waite, J Herbert; Chen, Zhan
2003-08-20
In this Communication, we demonstrate the novel observation that it is feasible to collect amide signals from polymer/protein solution interfaces in situ using sum frequency generation (SFG) vibrational spectroscopy. Such SFG amide signals allow for acquisition of more detailed molecular level information of entire interfacial protein structures. Proteins investigated include bovine serum albumin, mussel protein mefp-2, factor XIIa, and ubiquitin. Our studies indicate that different proteins generate different SFG amide signals at the polystyrene/protein solution interface, showing that they have different interfacial coverage, secondary structure, or orientation.
Liquid crystalline polymer nanocomposites reinforced with in-situ reduced graphene oxide
Directory of Open Access Journals (Sweden)
D. Pedrazzoli
2015-08-01
Full Text Available In this work liquid-crystalline polymer (LCP nanocomposites reinforced with in-situ reduced graphene oxide are investigated. Graphene oxide (GO was first synthesized by the Hummers method, and the kinetics of its thermal reduction was assessed. GO layers were then homogeneously dispersed in a thermotropic liquid crystalline polymer matrix (Vectran®, and an in-situ thermal reduction of GO into reduced graphene oxide (rGO was performed. Even at low rGO amount, the resulting nanocomposites exhibited an enhancement of both the mechanical properties and the thermal stability. Improvements of the creep stability and of the thermo-mechanical behavior were also observed upon nanofiller incorporation. Furthermore, in-situ thermal reduction of the insulating GO into the more electrically conductive rGO led to an important surface resistivity decrease in the nanofilled samples.
Visualization of thermally activated nanocarriers using in situ atomic force microscopy
DEFF Research Database (Denmark)
Dong, M. D.; Howard, K. A.; Oupicky, D.
2007-01-01
Thermo-responsive nanocarriers aim to improve the delivery of drugs into target tissue by a process of size-mediated deposition activated by thermal stimuli. The direct imaging of thermally-induced changes in nanocarrier morphology was demonstrated using in situ liquid AFM over a nano-scale and t......-scale and temperature range relevant for clinical approaches. In situ AFM proved to be a unique method for investigating the dynamic conformational changes of individual nanoparticles, promoting its application in the future development of stimuli-responsive nanocarriers.......Thermo-responsive nanocarriers aim to improve the delivery of drugs into target tissue by a process of size-mediated deposition activated by thermal stimuli. The direct imaging of thermally-induced changes in nanocarrier morphology was demonstrated using in situ liquid AFM over a nano...
Directory of Open Access Journals (Sweden)
Antoine R. M. Dalod
2017-12-01
Full Text Available Organic-inorganic hybrid materials are attractive due to the combination of properties from the two distinct types of materials. In this work, transparent titania-polydimethylsiloxane hybrid materials with up to 15.5 vol. % TiO2 content were prepared by an in situ non-aqueous method using titanium (IV isopropoxide and hydroxy-terminated polydimethylsiloxane as precursors. Spectroscopy (Fourier transform infrared, Raman, Ultraviolet-visible, ellipsometry and small-angle X-ray scattering analysis allowed to describe in detail the structure and the optical properties of the nanocomposites. Titanium alkoxide was successfully used as a cross-linker and titania-like nanodomains with an average size of approximately 4 nm were shown to form during the process. The resulting hybrid nanocomposites exhibit high transparency and tunable refractive index from 1.42 up to 1.56, depending on the titania content.
Ohta, Reiya; Fujioka, Hiromichi
2017-01-01
Recent progress in the chemoselective reduction and alkylation of carbonyl functions using our in situ protection method is described. Methods that enable reversal or control of the reactivity of a carbonyl functional group are potentially useful. They open up new areas of synthetic organic chemistry and change the concept of retrosynthesis because they remove the need for complicated protection/deprotection sequences. In this account, we discuss the strategy and applications of our in situ protection method using phosphonium salts.
Improvement of the accuracy of noise measurements by the two-amplifier correlation method.
Pellegrini, B; Basso, G; Fiori, G; Macucci, M; Maione, I A; Marconcini, P
2013-10-01
We present a novel method for device noise measurement, based on a two-channel cross-correlation technique and a direct "in situ" measurement of the transimpedance of the device under test (DUT), which allows improved accuracy with respect to what is available in the literature, in particular when the DUT is a nonlinear device. Detailed analytical expressions for the total residual noise are derived, and an experimental investigation of the increased accuracy provided by the method is performed.
In situ deposition of hydroxyapatite on graphene nanosheets
International Nuclear Information System (INIS)
Neelgund, Gururaj M.; Oki, Aderemi; Luo, Zhiping
2013-01-01
Graphical abstract: A facile chemical precipitation method is reported for effective in situ deposition of hydroxyapatite on graphene nanosheets. Prior to grafting of hydroxyapatite, chemically modified graphene nanosheets were obtained by the reduction of graphene oxide in presence of ethylenediamine. Display Omitted Highlights: ► It is a facile and effective method for deposition of HA on GR nanosheets. ► It avoids the use of harmful reducing agents like hydrazine, NaBH 4 etc. ► GR nanosheets were produced using bio-compatible, ethylenediamine. ► The graphitic structure of synthesized GR nanosheets was high ordered. ► The ratio of Ca to P in HA was 1.64, which is close to ratio in natural bone. -- Abstract: Graphene nanosheets were effectively functionalized by in situ deposition of hydroxyapatite through a facile chemical precipitation method. Prior to grafting of hydroxyapatite, chemically modified graphene nanosheets were obtained by the reduction of graphene oxide in presence of ethylenediamine. The resulting hydroxyapatite functionalized graphene nanosheets were characterized by attenuated total reflection IR spectroscopy, X-ray diffraction, field emission scanning electron microscopy, transmission electron microscopy, X-ray energy dispersive spectroscopy, Raman spectroscopy and thermogravimetric analysis. These characterization techniques revealed the successful grafting of hydroxyapatite over well exfoliated graphene nanosheets without destroying their structure.
In situ deposition of hydroxyapatite on graphene nanosheets
Energy Technology Data Exchange (ETDEWEB)
Neelgund, Gururaj M. [Department of Chemistry, Prairie View A and M University, Prairie View, TX 77446 (United States); Oki, Aderemi, E-mail: aroki@pvamu.edu [Department of Chemistry, Prairie View A and M University, Prairie View, TX 77446 (United States); Luo, Zhiping [Microscopy and Imaging Center and Materials Science and Engineering Program, Texas A and M University, College Station, TX 77843 (United States)
2013-02-15
Graphical abstract: A facile chemical precipitation method is reported for effective in situ deposition of hydroxyapatite on graphene nanosheets. Prior to grafting of hydroxyapatite, chemically modified graphene nanosheets were obtained by the reduction of graphene oxide in presence of ethylenediamine. Display Omitted Highlights: ► It is a facile and effective method for deposition of HA on GR nanosheets. ► It avoids the use of harmful reducing agents like hydrazine, NaBH{sub 4} etc. ► GR nanosheets were produced using bio-compatible, ethylenediamine. ► The graphitic structure of synthesized GR nanosheets was high ordered. ► The ratio of Ca to P in HA was 1.64, which is close to ratio in natural bone. -- Abstract: Graphene nanosheets were effectively functionalized by in situ deposition of hydroxyapatite through a facile chemical precipitation method. Prior to grafting of hydroxyapatite, chemically modified graphene nanosheets were obtained by the reduction of graphene oxide in presence of ethylenediamine. The resulting hydroxyapatite functionalized graphene nanosheets were characterized by attenuated total reflection IR spectroscopy, X-ray diffraction, field emission scanning electron microscopy, transmission electron microscopy, X-ray energy dispersive spectroscopy, Raman spectroscopy and thermogravimetric analysis. These characterization techniques revealed the successful grafting of hydroxyapatite over well exfoliated graphene nanosheets without destroying their structure.
International Nuclear Information System (INIS)
Ding, Chaoying; Tian, Li; Liu, Bo; Liang, Qian; Li, Zhongyu; Xu, Song; Liu, Qiaoli; Lu, Dayong
2015-01-01
Highlights: • MWCNT/SnIn 4 S 8 composites were facilely fabricated via in situ solvothermal method. • MWCNT/SnIn 4 S 8 composites exhibited significantly enhanced visible-light activity. • MWCNT/SnIn 4 S 8 composites showed remarkable visible light photocatalytic activity. • MWCNT/SnIn 4 S 8 composites exhibited excellent photo-stability. • Possible photocatalytic mechanism under visible-light irradiation was proposed. - Abstract: Superior photocatalytic activity could be achieved by multi-walled carbon nanotube (MWCNT) incorporated in the porous assembly of marigold-like SnIn 4 S 8 heterostructures synthesized by a flexible in-situ solvothermal method. The as-prepared MWCNT/SnIn 4 S 8 composites were well-characterized by powder X-ray diffraction (XRD), transmission electron microscopy (TEM), field emission scanning electron microscopy (FE-SEM) and UV–vis diffuse reflectance spectroscopy (DRS). The photocatalytic properties of the as-prepared samples were tested by photo-degradation of aqueous malachite green (MG) under the irradiation of visible light. It was found that the MWCNT/SnIn 4 S 8 composites showed enhanced visible light photocatalytic activity for dye degradation, and an optimum photocatalytic activity was observed over 3.0 wt.% MWCNT incorporated SnIn 4 S 8 composites. The superior photocatalytic activity of MWCNT/SnIn 4 S 8 composites could be ascribed to the existence of MWCNT which could serve as a good electron acceptor, mediator as well as the co-catalyst for dye degradation. The synergistic effect between SnIn 4 S 8 and MWCNT in the composites facilitated the interfacial charge transfer driven by the excitation of SnIn 4 S 8 under visible-light irradiation. Furthermore, a possible mechanism for the photocatalytic degradation of MWCNT/SnIn 4 S 8 composites was also discussed
Energy Technology Data Exchange (ETDEWEB)
Li, Meimei [Argonne National Lab. (ANL), Argonne, IL (United States); Almer, Jonathan D. [Argonne National Lab. (ANL), Argonne, IL (United States); Yang, Yong [Univ. of Florida, Gainesville, FL (United States); Tan, Lizhen [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
2016-01-01
This report provides a summary of research activities on understanding microstructure – property correlation in reactor materials using in situ high-energy X-rays. The report is a Level 2 deliverable in FY16 (M2CA-13-IL-AN_-0403-0111), under the Work Package CA-13-IL-AN_- 0403-01, “Microstructure-Property Correlation in Reactor Materials using in situ High Energy Xrays”, as part of the DOE-NE NEET Program. The objective of this project is to demonstrate the application of in situ high energy X-ray measurements of nuclear reactor materials under thermal-mechanical loading, to understand their microstructure-property relationships. The gained knowledge is expected to enable accurate predictions of mechanical performance of these materials subjected to extreme environments, and to further facilitate development of advanced reactor materials. The report provides detailed description of the in situ X-ray Radiated Materials (iRadMat) apparatus designed to interface with a servo-hydraulic load frame at beamline 1-ID at the Advanced Photon Source. This new capability allows in situ studies of radioactive specimens subject to thermal-mechanical loading using a suite of high-energy X-ray scattering and imaging techniques. We conducted several case studies using the iRadMat to obtain a better understanding of deformation and fracture mechanisms of irradiated materials. In situ X-ray measurements on neutron-irradiated pure metal and model alloy and several representative reactor materials, e.g. pure Fe, Fe-9Cr model alloy, 316 SS, HT-UPS, and duplex cast austenitic stainless steels (CASS) CF-8 were performed under tensile loading at temperatures of 20-400°C in vacuum. A combination of wide-angle X-ray scattering (WAXS), small-angle X-ray scattering (SAXS), and imaging techniques were utilized to interrogate microstructure at different length scales in real time while the specimen was subject to thermal-mechanical loading. In addition, in situ X-ray studies were
Stability of added and in situ-produced vitamin B12 in breadmaking.
Edelmann, Minnamari; Chamlagain, Bhawani; Santin, Marco; Kariluoto, Susanna; Piironen, Vieno
2016-08-01
Vitamin B12 exists naturally in foods of animal origin and is synthesised only by certain bacteria. New food sources are needed to ensure vitamin B12 intake in risk groups. This study aimed to investigate the stability of added cyanocobalamin (CNCbl, chemically modified form) and hydroxocobalamin (OHCbl, natural form) and in situ-synthesised vitamin B12 in breadmaking. Samples were analysed both with a microbiological (MBA) and a liquid chromatographic (UHPLC) method to test applicability of these two methods. Proofing did not affect CNCbl and OHCbl levels. By contrast, 21% and 31% of OHCbl was lost in oven-baking steps in straight- and sponge-dough processes, respectively, whereas CNCbl remained almost stable. In sourdough baking, 23% of CNCbl and 44% of OHCbl were lost. In situ-produced vitamin B12 was almost as stable as added CNCbl and more stable than OHCbl. The UHPLC method showed its superiority to the MBA in determining the active vitamin B12. Copyright © 2016. Published by Elsevier Ltd.
Energy Technology Data Exchange (ETDEWEB)
Li, D.T.; Li, W.; Sun, Q.L.; Li, B.Q. [Chinese Academy of Science, Taiyuan (China). Inst. for Coal Chemistry
2003-04-01
A new method using the small porcelain as the reactor combined with increasing the flow rate of carrier gas was proposed, which has the advantage of excluding the condensation of volatile produced by heated solid samples on the windows during in situ diffuse reflectance FTIR experiment. Moreover, the feasibility of this method was also discussed. Using this method, the distribution and thermal stability of hydrogen bonds in coal macerals obtained from two coals were studied. The results show that the differences between the distribution of hydrogen bonds formed by hydroxyl group in the macerals of two coals were very similar. For the vitrinites the thermal stability of SH-N, carboxylic acid dimmers and self-associated OH is higher than those in inertinites but for OH-N and hydroxyl tetramers and OH-OR{sub 2} there are no obvious laws. For OH-{pi}, its content increased with increasing temperature to 350-380{sup o}C, and then decreased with further heating. The variation of hydrogen bonds in macerals reflects the difference in their structure.
Optimization and evaluation of thermoresponsive diclofenac sodium ophthalmic in situ gels.
Asasutjarit, Rathapon; Thanasanchokpibull, Suthira; Fuongfuchat, Asira; Veeranondha, Sukitaya
2011-06-15
This work was conducted to optimize and evaluate Pluronic F127-based thermoresponsive diclofenac sodium ophthalmic in situ gels (DS in situ gel). They were prepared by cold method and investigated their physicochemical properties i.e., pH, flow ability, sol-gel transition temperature, gelling capacity and rheological properties. An optimized formulation was selected and investigated its physicochemical properties before and after autoclaving, eye irritation potency in SIRC cells and rabbits. In vivo ophthalmic absorption was performed in rabbits. It was found that physicochemical properties of DS in situ gels were affected by formulation compositions. Increment of Pluronic F127 content decreased sol-gel transition temperature of the products while increase in Pluronic F68 concentration tended to increase sol-gel transition temperature. In this study, Carbopol 940 did not affect sol-gel transition temperature but it affected transparency, pH, and gelling capacity of the products. The optimized formulation exhibited sol-gel transition at 32.6 ± 1.1 °C with pseudoplastic flow behavior. It was lost diclofenac sodium content during autoclaving. However, it was accepted as safe for ophthalmic use and could increase diclofenac sodium bioavailability in aqueous humor significantly. In conclusion, the optimized DS in situ gel had potential for using as an alternative to the conventional diclofenac sodium eye drop. However, autoclaving was not a suitable sterilization method for this product. Copyright © 2011 Elsevier B.V. All rights reserved.
Energy Technology Data Exchange (ETDEWEB)
Munson, D.E.; Matalucci, R.V. [Sandia National Lab., Albuquerque, NM (United States); Hoag, D.L.; Blankenship D.A. [RE/SPEC Inc., Albuquerque, NM (United States)] [and others
1997-02-01
The Department of Energy has constructed the Waste Isolation Pilot Plant (WIPP) to develop the technology for the disposal of radioactive waste from defense programs. Sandia National Laboratories has the responsibility for experimental activities at the WIPP and has emplaced several large-scale Thermal/Structural Interactions (TSI) in situ tests to validate techniques used to predict repository performance. The construction of the tests relied heavily on earlier excavations at the WIPP site to provide a basis for selecting excavation, surveying, and instrumentation methods, and achievable construction tolerances. The tests were constructed within close tolerances to provide consistent room dimensions and accurate placement of gages. This accuracy has contributed to the high quality of data generated which in turn has facilitated the comparison of test results to numerical predictions. The purpose of this report is to detail the construction activities of the TSI tests.
International Nuclear Information System (INIS)
Munson, D.E.; Matalucci, R.V.; Hoag, D.L.; Blankenship D.A.
1997-02-01
The Department of Energy has constructed the Waste Isolation Pilot Plant (WIPP) to develop the technology for the disposal of radioactive waste from defense programs. Sandia National Laboratories has the responsibility for experimental activities at the WIPP and has emplaced several large-scale Thermal/Structural Interactions (TSI) in situ tests to validate techniques used to predict repository performance. The construction of the tests relied heavily on earlier excavations at the WIPP site to provide a basis for selecting excavation, surveying, and instrumentation methods, and achievable construction tolerances. The tests were constructed within close tolerances to provide consistent room dimensions and accurate placement of gages. This accuracy has contributed to the high quality of data generated which in turn has facilitated the comparison of test results to numerical predictions. The purpose of this report is to detail the construction activities of the TSI tests
In situ process monitoring in selective laser sintering using optical coherence tomography
Gardner, Michael R.; Lewis, Adam; Park, Jongwan; McElroy, Austin B.; Estrada, Arnold D.; Fish, Scott; Beaman, Joseph J.; Milner, Thomas E.
2018-04-01
Selective laser sintering (SLS) is an efficient process in additive manufacturing that enables rapid part production from computer-based designs. However, SLS is limited by its notable lack of in situ process monitoring when compared with other manufacturing processes. We report the incorporation of optical coherence tomography (OCT) into an SLS system in detail and demonstrate access to surface and subsurface features. Video frame rate cross-sectional imaging reveals areas of sintering uniformity and areas of excessive heat error with high temporal resolution. We propose a set of image processing techniques for SLS process monitoring with OCT and report the limitations and obstacles for further OCT integration with SLS systems.
An overview of in situ waste treatment technologies
International Nuclear Information System (INIS)
Walker, S.; Hyde, R.A.; Piper, R.B.; Roy, M.W.
1992-01-01
In situ technologies are becoming an attractive remedial alternative for eliminating environmental problems. In situ treatments typically reduce risks and costs associated with retrieving, packaging, and storing or disposing-waste and are generally preferred over ex situ treatments. Each in situ technology has specific applications, and, in order to provide the most economical and practical solution to a waste problem, these applications must be understood. This paper presents an overview of thirty different in situ remedial technologies for buried wastes or contaminated soil areas. The objective of this paper is to familiarize those involved in waste remediation activities with available and emerging in situ technologies so that they may consider these options in the remediation of hazardous and/or radioactive waste sites. Several types of in situ technologies are discussed, including biological treatments, containment technologies, physical/chemical treatments, solidification/stabilization technologies, and thermal treatments. Each category of in situ technology is briefly examined in this paper. Specific treatments belonging to these categories are also reviewed. Much of the information on in situ treatment technologies in this paper was obtained directly from vendors and universities and this information has not been verified
In situ measurement of diffusivity
International Nuclear Information System (INIS)
Berne, F.; Pocachard, J.
2004-01-01
The mechanism of molecular diffusion controls the migration of contaminants in very low-permeability porous media, like underground facilities for the storage of hazardous waste. Determining of relevant diffusion coefficients is therefore of prime importance. A few techniques exist for in situ measurement of the quantity, but they suffer from many handicaps (duration, complexity and cost of the experiments). We propose here two innovative methods that have some potential to improve the situation. So far, we have found them feasible on the basis of design calculations and laboratory experiments. This work is presently protected by a patent. (author)
In situ measurement of diffusivity
International Nuclear Information System (INIS)
Berne, Ph.; Pocachard, J.
2005-01-01
The mechanism of molecular diffusion controls the migration of contaminants in very low-permeability porous media, like underground facilities for the storage of hazardous waste. Determining the relevant diffusion coefficients is, therefore, of prime importance. A few techniques exist for the in situ measurement of that quantity, but they suffer from many handicaps (duration, complexity and cost of the experiments). We propose here two innovative methods that have some potential to improve this situation. So far, we have found them feasible on the basis of design calculations and laboratory experiments. This work is presently protected by a patent. (author)
International Nuclear Information System (INIS)
Ragsdale, H.L.; Tanner, B.K.; Coleman, R.N.; Palms, J.M.; Wood, R.E.
1978-01-01
In situ and laboratory gamma-ray spectroscopy measurements were taken in nine scrub oak forests and nine old fields to determine the applicability of in situ analysis in the coastal plain. Data collected at each of the 18 sites included a 2-hr count, soil density and moisture estimates, and vegetation measurements. Samples returned to the laboratory for radiometric analysis included litter and herbaceous vegetation and soil cores. Analysis of the gamma-emitter detection frequencies, concentrations, and burdens showed good to excellent agreement between laboratory and in situ methods. Generally, forests were determined to be superior in situ sampling systems. Laboratory analysis of collected samples may be a superior technique for gamma emitters with low energies, low concentrations, or nonuniform distributions in the soil. Three potential uses of in situ Ge(Li) spectrometers were identified and discussed in terms of their limits and of the replicate ecosystems appropriate for in situ analyses. Although the variety and the biogeochemical cycling regimes of southeastern coastal plain ecosystems complicate in situ analyses, it was concluded that comparable and probably accurate results can be achieved using in situ technology
International Nuclear Information System (INIS)
Wan, Caichao; Jiao, Yue; Li, Jian
2015-01-01
Graphical abstract: - Highlights: • The graphene/wood nanocomposites were fabricated via a mild fast one-pot hydrothermal method. • Graphene nanosheets were in situ deposited on the surface of the wood matrix. • The incorporation of graphene nanosheets results in the improvement in the thermal stability of the graphene/wood. • The graphene/wood exhibits superior UV resistance performance. - Abstract: Graphene nanosheets were successfully in situ deposited on the surface of the wood matrix via a mild fast one-pot hydrothermal method, and the resulting hybrid graphene/wood (GW) were characterized by scanning electron microscope (SEM), energy dispersive X-ray spectroscopy (EDX), X-ray diffraction (XRD), Raman spectroscopy, and thermogravimetric analysis (TGA). According to the results, the wood matrix was evenly coated by dense uninterrupted multilayer graphene membrane structure, which was formed by layer-by-layer self-assembly of graphene nanosheets. Meanwhile, the graphene coating also induced significant improvement in the thermal stability of GW in comparison with that of the original wood (OW). Accelerated weathering tests were employed to measure and determine the UV-resistant ability of OW and GW. After about six hundred hours of experiments, the surface color change of GW was much less than that of OW; besides, the Fourier transform infrared spectroscopy (FTIR) analysis also proved the less significant changes in surface chemical compositions of GW. The results both indicated that the graphene coating effectively protected wood surface from UV damage. Therefore, this class of GW composite might be expected to be served as high-performance wooden building material for outdoor or some particular harsh environments like strong UV radiation regions use
Souder, Christopher D; Bomar, James D; Wenger, Dennis R
2014-12-01
Slipped capital femoral epiphysis (SCFE) can be treated by a variety of methods with the traditional method of in situ pin fixation being most commonly used. More recently, the Modified Dunn (Mod. Dunn) procedure consisting of capital realignment has been popularized as a treatment method for SCFE, particularly for more severe cases. Over the last 5 years, our institution has selectively used this method for more complex cases. The purpose of this article is to evaluate the differences between these 2 treatment methods in terms of avascular necrosis (AVN) rate, reoperation rate, and complication rate. Eighty-eight hips that were surgically treated for SCFE between July 2004 and June 2012 met our inclusion criteria. The in situ fixation group included 71 hips, whereas 17 hips were anatomically reduced with the Mod. Dunn procedure. Loder classification, severity, acuity, complication rate, and reoperation rate were determined for the 2 cohorts. The χ analysis was performed to evaluate the relationship between the treatment method and outcome. As expected, stable slips did well with in situ pinning with no cases of AVN, even in more severe slips. Ten stable slips were treated with the Mod. Dunn approach and 2 (20%) developed AVN. Unstable slips were more difficult to treat with 3 of the 7 hips stabilized in situ developing AVN (43%). Two of the 7 unstable slips treated by the Mod. Dunn procedure developed AVN (29%). The other outcomes studied (reoperation rate and complication rate) were not significantly related to the surgical treatment method (P = 0.732 and 0.261, respectively). In situ pinning remains a safe and predictable method for treatment of stable SCFE with no AVN noted, even in severe slips. Attempts to anatomically reduce stable slips led to severe AVN in 20% of cases, thus this treatment approach should be considered with caution. Treatment of unstable slips remains problematic with high AVN rates noted whether treated by in situ fixation or capital
Clinical system for non-invasive in situ monitoring of gases in the human paranasal sinuses.
Lewander, Märta; Guan, Zuguang; Svanberg, Katarina; Svanberg, Sune; Svensson, Tomas
2009-06-22
We present a portable system for non-invasive, simultaneous sensing of molecular oxygen (O(2)) and water vapor (H(2)O) in the human paranasal cavities. The system is based on high-resolution tunable diode laser spectroscopy (TDLAS) and digital wavelength modulation spectroscopy (dWMS). Since optical interference and non-ideal tuning of the diode lasers render signal processing complex, we focus on Fourier analysis of dWMS signals and procedures for removal of background signals. Clinical data are presented, and exhibit a significant improvement in signal-to-noise with respect to earlier work. The in situ detection limit, in terms of absorption fraction, is about 5x10(-5) for oxygen and 5x10(-4) for water vapor, but varies between patients due to differences in light attenuation. In addition, we discuss the use of water vapor as a reference in quantification of in situ oxygen concentration in detail. In particular, light propagation aspects are investigated by employing photon time-of-flight spectroscopy.
Pi, Jiang; Jin, Hua; Yang, Fen; Chen, Zheng W.; Cai, Jiye
2014-10-01
The cell membrane, which consists of a viscous phospholipid bilayer, different kinds of proteins and various nano/micrometer-sized domains, plays a very important role in ensuring the stability of the intracellular environment and the order of cellular signal transductions. Exploring the precise cell membrane structure and detailed functions of the biomolecules in a cell membrane would be helpful to understand the underlying mechanisms involved in cell membrane signal transductions, which could further benefit research into cell biology, immunology and medicine. The detection of membrane biomolecules at the single molecule level can provide some subtle information about the molecular structure and the functions of the cell membrane. In particular, information obtained about the molecular mechanisms and other information at the single molecule level are significantly different from that detected from a large amount of biomolecules at the large-scale through traditional techniques, and can thus provide a novel perspective for the study of cell membrane structures and functions. However, the precise investigations of membrane biomolecules prompts researchers to explore cell membranes at the single molecule level by the use of in situ imaging methods, as the exact conformation and functions of biomolecules are highly controlled by the native cellular environment. Recently, the in situ single molecule imaging of cell membranes has attracted increasing attention from cell biologists and immunologists. The size of biomolecules and their clusters on the cell surface are set at the nanoscale, which makes it mandatory to use high- and super-resolution imaging techniques to realize the in situ single molecule imaging of cell membranes. In the past few decades, some amazing imaging techniques and instruments with super resolution have been widely developed for molecule imaging, which can also be further employed for the in situ single molecule imaging of cell membranes. In
Vasquez, Joshua J; Hussien, Rajaa; Aguilar-Rodriguez, Brandon; Junger, Henrik; Dobi, Dejan; Henrich, Timothy J; Thanh, Cassandra; Gibson, Erica; Hogan, Louise E; McCune, Joseph; Hunt, Peter W; Stoddart, Cheryl A; Laszik, Zoltan G
2018-02-01
Persistent tissue reservoirs of HIV present a major barrier to cure. Defining subsets of infected cells in tissues is a major focus of HIV cure research. Herein, we describe a novel multiplexed in situ hybridization (ISH) (RNAscope) protocol to detect HIV-DNA (vDNA) and HIV-RNA (vRNA) in formalin-fixed paraffin-embedded (FFPE) human tissues in combination with immunofluorescence (IF) phenotyping of the infected cells. We show that multiplexed IF and ISH (mIFISH) is suitable for quantitative assessment of HIV vRNA and vDNA and that multiparameter IF phenotyping allows precise identification of the cellular source of the ISH signal. We also provide semi-quantitative data on the impact of various tissue fixatives on the detectability of vDNA and vRNA with RNAscope technology. Finally, we describe methods to quantitate the ISH signal on whole-slide digital images and validation of the quantitative ISH data with quantitative real-time PCR for vRNA. It is our hope that this approach will provide insight into the biology of HIV tissue reservoirs and to inform strategies aimed at curing HIV.
Nuclear Weak Rates and Detailed Balance in Stellar Conditions
Energy Technology Data Exchange (ETDEWEB)
Misch, G. Wendell, E-mail: wendell@sjtu.edu, E-mail: wendell.misch@gmail.com [Department of Physics and Astronomy, Shanghai Jiao Tong University, 800 Dong Chuan Road, Shanghai 200240 (China)
2017-07-20
Detailed balance is often invoked in discussions of nuclear weak transitions in astrophysical environments. Satisfaction of detailed balance is rightly touted as a virtue of some methods of computing nuclear transition strengths, but I argue that it need not necessarily be strictly obeyed in astrophysical environments, especially when the environment is far from weak equilibrium. I present the results of shell model calculations of nuclear weak strengths in both charged-current and neutral-current channels at astrophysical temperatures, finding some violation of detailed balance. I show that a slight modification of the technique to strictly obey detailed balance has little effect on the reaction rates associated with these strengths under most conditions, though at high temperature the modified technique in fact misses some important strength. I comment on the relationship between detailed balance and weak equilibrium in astrophysical conditions.
Method of repair of short circuits for in-situ leaching
International Nuclear Information System (INIS)
Baughman, D.R.; Bergeson, J.R.
1984-01-01
In an acidic in-situ leaching system, a short circuit passage through a subterranean formation between a fracture associated with an injection well and a fracture associated with a production well can be plugged by introducing a non-acidic liquid for displacing acidic leach liquid from the short circuit passage, introducing into the injection well a basic composition including a sealing material that gels under acidic conditions, and introducing sufficient liquid into the injection well to displace at least a portion of the basic composition containing sealing material from the injection well into the short circuit passage. Liquid flow between the injection well and the production well is then discontinued for a sufficient time for residual acid in the subterranean formation surrounding the short circuit passage to contact the sealing material and cause gelation of the sealing material in the short circuit passage. The introduction of acidic leach liquid to the formation can then continue. The sealing material may be a polymer or a water soluble silicate
International Nuclear Information System (INIS)
Smith, W.H.
1992-10-01
Two commercially available chloride-ion-specific electrodes (CLISEs), a solid-state type and a membrane type, were evaluated as potential in situ chemical sensors for determining total chloride ion concentration in mixed sodium chloride/potassium chloride/hydrochloric acid solutions generated during the recovery of plutonium from molten salts used in plutonium electrorefining operations. Because the response of the solid-state CLISE was closer than was the response of the membrane-type CLISE to the theoretical response predicted by the Nernst equation, the solid-state CLISE was selected for further evaluation. A detailed investigation of the characteristics of the chloride system and the corresponding CLISE response to concentration changes suggested four methods by which the CLISE could be used either as a direct, in situ sensor or as an indirect sensor through which an analysis could be performed on-line with a sample extracted from the process solution
Directory of Open Access Journals (Sweden)
Yijian Zeng
2016-03-01
Full Text Available The inter-comparison of different soil moisture (SM products over the Tibetan Plateau (TP reveals the inconsistency among different SM products, when compared to in situ measurement. It highlights the need to constrain the model simulated SM with the in situ measured data climatology. In this study, the in situ soil moisture networks, combined with the classification of climate zones over the TP, were used to produce the in situ measured SM climatology at the plateau scale. The generated TP scale in situ SM climatology was then used to scale the model-simulated SM data, which was subsequently used to scale the SM satellite observations. The climatology-scaled satellite and model-simulated SM were then blended objectively, by applying the triple collocation and least squares method. The final blended SM can replicate the SM dynamics across different climatic zones, from sub-humid regions to semi-arid and arid regions over the TP. This demonstrates the need to constrain the model-simulated SM estimates with the in situ measurements before their further applications in scaling climatology of SM satellite products.
Eizenberg, H; Shtienberg, D; Silberbush, M; Ephrath, J E
2005-11-01
To develop an in-situ, non-destructive method for observation and monitoring of the underground developmental stages of the root parasite Orobanche cumana. The parasitic weed Orobanche causes severe damage to vegetables and field crops. Most of the damage caused to the crops occurs during the underground, unobservable parasitism stage. Sunflower (Helianthus annuus 'Adi') plants were planted in soil that was artificially inoculated with O. cumana seeds. Clear Plexiglas mini-rhizotron plastic observation tubes were inserted into the soil. Seed germination, early stage of penetration, and formation of tubercles and spikes were observed non-destructively and were monitored throughout the growing season by mean of a mini-rhizotron camera. Use of this technology enabled the complete individual parasite life cycle from the very early development (including germination) to Orobanche shoot to be monitored. In addition, the effect of the systemic herbicide Cadre (imazapic) on the development of O. cumana was inspected and quantified. This novel methodology facilitates the in-situ study of major aspects of the host-parasite interaction and of parasite suppression, such as parasitism dynamics, parasite growth rate, and the effect of chemical treatments on the parasite.
In Situ Field Testing of Processes
Energy Technology Data Exchange (ETDEWEB)
J. Wang
2001-12-14
The purpose of this Analysis/Model Report (AMR) is to update and document the data and subsequent analyses from ambient field-testing activities performed in underground drifts of the Yucca Mountain Site Characterization Project (YMP). This revision updates data and analyses presented in the initial issue of this AMR. This AMR was developed in accordance with the ''Technical Work Plan for Unsaturated Zone (UZ) Flow and Transport Process Model Report'' and ''Technical Work Plan for UZ Flow, Transport, and Coupled Processes Process Model Report. These activities were performed to investigate in situ flow and transport processes. The evaluations provide the necessary framework to: (1) refine and confirm the conceptual model of matrix and fracture processes in the unsaturated zone (UZ) and (2) analyze the impact of excavation (including use of construction water and effect of ventilation) on the UZ flow and transport processes. This AMR is intended to support revisions to ''Conceptual and Numerical Models for UZ Flow and Transport'' and ''Unsaturated Zone Flow and Transport Model Process Model Report''. In general, the results discussed in this AMR are from studies conducted using a combination or a subset of the following three approaches: (1) air-injection tests, (2) liquid-release tests, and (3) moisture monitoring using in-drift sensors or in-borehole sensors, to evaluate the impact of excavation, ventilation, and construction-water usage on the surrounding rocks. The liquid-release tests and air-injection tests provide an evaluation of in situ fracture flow and the competing processes of matrix imbibition. Only the findings from testing and data not covered in the ''Seepage Calibration Model and Seepage Testing Data'' are analyzed in detail in the AMR.
Kawasaki, M; Sasaki, K; Satoh, T; Kurose, A; Kamada, T; Furuya, T; Murakami, T; Todoroki, T
1997-01-01
We have demonstrated a method for the in situ determination of the cell cycle phases of TIG-7 fibroblasts using a laser scanning cytometer (LSC) which has not only a function equivalent to flow cytometry (FCM) but also has a capability unique in itself. LSC allows a more detailed analysis of the cell cycle in cells stained with propidium iodide (PI) than FCM. With LSC it is possible to discriminate between mitotic cells and G2 cells, between post-mitotic cells and G1 cells, and between quiescent cells and cycling cells in a PI fluorescence peak (chromatin condensation) vs. fluorescence value (DNA content) cytogram for cells stained with PI. These were amply confirmed by experiments using colcemid and adriamycin. We were able to identify at least six cell subpopulations for PI stained cells using LSC; namely G1, S, G2, M, postmitotic and quiescent cell populations. LSC analysis facilitates the monitoring of effects of drugs on the cell cycle.
International Nuclear Information System (INIS)
Xiao Xuefu; Song Lijun; Wang Yulai; Wen Fuping; Liao Haitao; Ban Ying; Xia Yihua; Li Ruixiang; Li Hang; Tu Xingmin
2007-06-01
A new calibration technique, which is the Monte Carlo modeling technique, of in-situ HPGe γ spectrometers used for non-destructive analyzing radioactivity in nuclear facilities decommissioning, is presented. A series of assay for some stainless steel pipes and tanks in some nuclear facilities/laboratories of CIAE are taken on site with the in-situ HPGe γ spectrometer. At the same time, some examples are taken and analyzed in laboratories. The relative bias/variation between the values of activity measured by in-situ HPGe γ spectrometers on site and that analyzed in laboratory is less than ±45.0%. (authors)
Yu, Xiao-Ying; Liu, Bingwen; Yang, Li; Zhu, Zihua; Marshall, Matthew J.
2016-03-01
A microfluidic electrochemical device and process are detailed that provide chemical imaging and electrochemical analysis under vacuum at the surface of the electrode-sample or electrode-liquid interface in-situ. The electrochemical device allows investigation of various surface layers including diffuse layers at selected depths populated with, e.g., adsorbed molecules in which chemical transformation in electrolyte solutions occurs.
Giger-Reverdin, Sylvie; Maaroufi, Chiraze; Chapoutot, Patrick; Peyronnet, Corinne; Sauvant, Daniel
2014-01-01
In ruminant nutrition, peas are characterized by high protein solubility and degradability, which impair its protein value estimated by the official in situ method. Grinding can be used as a technological treatment of pea seeds to modify their nutritional value. The aim of this study was to compare the in situ method with an in vitro method on the same pea either in a coarse pea flour form (PCF) or in a ground pea fine flour form (PFF) to understand the effect of grinding. Both forms were also reground (GPCF and GPFF). PCF presented a lower rate of in vitro degradation than PFF, and more stable fermentation parameters (pH, ammonia, soluble carbohydrates) even if gas production was higher for the PCF after 48 h of incubation. In situ dry matter and protein degradation were lower for PCF than those for PFF; these differences were more marked than with the in vitro method. Reground peas were very similar to PFF. The values for pea protein digestible in the intestine (PDI) were higher for PCF than those for PFF. This study points out the high sensitivity of the in situ method to grinding. The study needs to be validated by in vivo measurements. PMID:25473488
In situ TEM observations of unusual nanocrystallization in a Ti-based bulk metallic glass
International Nuclear Information System (INIS)
Yan, M.; Wang, D.J.; Shen, J.; Qian, M.
2011-01-01
In situ transmission electron microscopy (TEM) has been used to witness the nanocrystallization of amorphous Ti 42.5 Cu 40 Zr 10 Ni 5 Sn 2.5 . A crystallization front exists to separate the TEM sample into two parts with different thermal stabilities. The number density of the crystallization products varies significantly, with the precipitate sizes ranging from a few nanometres to ∼100 nm. Detailed TEM analysis suggests that oxygen is the most likely reason for realizing the unusual nanocrystallization. External thermal analysis also indicates that oxygen affects the crystallization.
Directory of Open Access Journals (Sweden)
de Brevern Alexandre G
2005-09-01
Full Text Available Abstract Background A number of methods are now available to perform automatic assignment of periodic secondary structures from atomic coordinates, based on different characteristics of the secondary structures. In general these methods exhibit a broad consensus as to the location of most helix and strand core segments in protein structures. However the termini of the segments are often ill-defined and it is difficult to decide unambiguously which residues at the edge of the segments have to be included. In addition, there is a "twilight zone" where secondary structure segments depart significantly from the idealized models of Pauling and Corey. For these segments, one has to decide whether the observed structural variations are merely distorsions or whether they constitute a break in the secondary structure. Methods To address these problems, we have developed a method for secondary structure assignment, called KAKSI. Assignments made by KAKSI are compared with assignments given by DSSP, STRIDE, XTLSSTR, PSEA and SECSTR, as well as secondary structures found in PDB files, on 4 datasets (X-ray structures with different resolution range, NMR structures. Results A detailed comparison of KAKSI assignments with those of STRIDE and PSEA reveals that KAKSI assigns slightly longer helices and strands than STRIDE in case of one-to-one correspondence between the segments. However, KAKSI tends also to favor the assignment of several short helices when STRIDE and PSEA assign longer, kinked, helices. Helices assigned by KAKSI have geometrical characteristics close to those described in the PDB. They are more linear than helices assigned by other methods. The same tendency to split long segments is observed for strands, although less systematically. We present a number of cases of secondary structure assignments that illustrate this behavior. Conclusion Our method provides valuable assignments which favor the regularity of secondary structure segments.
Offset-gated poly-Si TFTs using in-situ fluorine passivation and excimer laser doping
International Nuclear Information System (INIS)
Jung, Sang Hoon; Kim, Cheon Hong; Yoo, Juhn Suk; Han, Min Koo
2000-01-01
A new low-temperature poly-Si thin film transistor (TFT) fabrication method employing in-situ fluorine passivation and excimer-laser doping is proposed to fabricate offset-gated poly-Si TFTs. In the new process, the crystallization, the in-situ fluorine passivation of the active layer, and the doping of the source/drain region are performed simultaneously with only one step of excimer laser annealing while the conventional fabrication method requires two laser annealing steps. Employing phosphosilicate glass (PSG) films as a diffusion source, we successfully accomplished excimer laser doping. The subthreshold and the on-state characteristics of the device with in-situ fluorine passivation were considerably improved. This improvement was due to the fluorine passivation effects, which cured dangling bonds and strained bonds in the poly-Si channel, the offset region, and the SiO 2 /poly-Si interface
Offset-gated poly-Si TFTs using in-situ fluorine passivation and excimer laser doping
Jung, S H; Yoo, J S; Han, M K
2000-01-01
A new low-temperature poly-Si thin film transistor (TFT) fabrication method employing in-situ fluorine passivation and excimer-laser doping is proposed to fabricate offset-gated poly-Si TFTs. In the new process, the crystallization, the in-situ fluorine passivation of the active layer, and the doping of the source/drain region are performed simultaneously with only one step of excimer laser annealing while the conventional fabrication method requires two laser annealing steps. Employing phosphosilicate glass (PSG) films as a diffusion source, we successfully accomplished excimer laser doping. The subthreshold and the on-state characteristics of the device with in-situ fluorine passivation were considerably improved. This improvement was due to the fluorine passivation effects, which cured dangling bonds and strained bonds in the poly-Si channel, the offset region, and the SiO sub 2 /poly-Si interface.
In-situ deposition of hydroxyapatite on graphene nanosheets
Neelgund, Gururaj M.; Oki, Aderemi; Luo, Zhiping
2013-01-01
Graphene nanosheets were effectively functionalized by in-situ deposition of hydroxyaptite through a facile chemical precipitation method. Prior to grafting of hydroxyapatite, chemically modified graphene nanosheets were obtained by the reduction of graphene oxide in presence of ethylenediamine. The resulting hydroxyapatite functionalized graphene nanosheets were characterized by attenuated total reflection IR spectroscopy, X-ray diffraction, field emission scanning electron microscopy, trans...
International Nuclear Information System (INIS)
He, Jichuan; Wang, Haibin; Gu, Chunlei; Liu, Shuxin
2014-01-01
Graphical abstract: The MoO 2 modified LiFePO 4 /C cathode materials were synthesized by in situ synthesis method. MoO 2 can sufficiently coat on the LiFePO 4 /C particles surface and does not alter LiFePO 4 crystal structure, and the adding of MoO 2 decreases the particles size and increases the tap density of cathode materials. The existence of MoO 2 improves electrochemical performance of LiFePO 4 cathode materials in specific capability and lithium ion diffusion and charge transfer resistance of cathode materials. - Highlights: • The MoO 2 modified LiFePO 4 /C cathode materials were synthesized by in situ synthesis method. • The existence of MoO 2 decreases the particles size and increases the tap density of cathode materials. • MoO 2 can sufficiently coat on the surface of LiFePO 4 /C cathode materials. • The existence of MoO 2 enhanced electrochemical performance of LiFePO 4 /C cathode materials. - Abstract: The MoO 2 modified LiFePO 4 /C cathode materials were synthesized by in situ synthesis method. Phase compositions and microstructures of the products were characterized by X-ray powder diffraction (XRD), SEM, TEM and EDS. Results indicate that MoO 2 can sufficiently coat on the LiFePO 4 surface and does not alter LiFePO 4 crystal structure, the existence of MoO 2 decreases the particles size and increases the tap density of cathode materials. The electrochemical behavior of cathode materials was analyzed using galvanostatic measurement, cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The results show that the existence of MoO 2 improves electrochemical performance of LiFePO 4 cathode materials in specific capability and lithium ion diffusion and charge transfer resistance. The initial charge–discharge specific capacity and apparent lithium ion diffusion coefficient increase, the charge transfer resistance decreases with MoO 2 content and maximizes around the MoO 2 content is 5 wt%. It has been had further proved that
Biofilm biodiversity presented by fluorescent in situ hybridisation
Directory of Open Access Journals (Sweden)
Wolf Mirela
2017-01-01
Full Text Available Numerous microorganisms may be present in the water distribution system. This is associated with the imperfection of purification processes, or secondary water pollution. Not only it results in the deterioration of water quality parameters, but it also increases threat of epidemiological problems. The water that is biologically unstable creates ideal conditions for colonization of the microorganisms to the inner surface of pipelines which may form biofilm. The key issue, enabling prevention and control of the impact of the development of biofilms, is to assess the biodiversity of microbiocenosis. In order to obtain comprehensive characteristics of microorganisms communities on a particular substrate, it is necessary to combine several techniques. Further analysis using molecular biology methods are usually after traditional methods of assessing the microbiological quality of water. Standard methods do not reflect the actual species composition, because they are targeted at the bacteria that can be isolated and cultured in the laboratory. Conventional methods are capable of detecting less than 10% of the organisms in the sample. In order to study the biodiversity of organisms inhabiting a biofilm (apart from the conventional methods analyses of the diversity of nucleic acids should be used. The first method could be the polymerase chain reaction (PCR and denaturing gradient gel electrophoresis (DGGE. Another way may be fluorescence in situ hybridization, which allows to detect determined DNA sequence using specially labeled oligonucleotide probes. Visualization of the material is performed using a fluorescence microscope. The main purpose of this article is to present rapid and precise identification groups of microorganisms in their natural habitat in biofilm using fluorescent in situ hybridization method (FISH . FISH method can be successfully used to visualize these microorganisms, which show difficulties in culturing, as well as to provide
Unintended and in situ amorphisation of pharmaceuticals.
Priemel, P A; Grohganz, H; Rades, T
2016-05-01
Amorphisation of poorly water-soluble drugs is one approach that can be applied to improve their solubility and thus their bioavailability. Amorphisation is a process that usually requires deliberate external energy input. However, amorphisation can happen both unintentionally, as in process-induced amorphisation during manufacturing, or in situ during dissolution, vaporisation, or lipolysis. The systems in which unintended and in situ amorphisation has been observed normally contain a drug and a carrier. Common carriers include polymers and mesoporous silica particles. However, the precise mechanisms by which in situ amorphisation occurs are often not fully understood. In situ amorphisation can be exploited and performed before administration of the drug or possibly even within the gastrointestinal tract, as can be inferred from in situ amorphisation observed during in vitro lipolysis. The use of in situ amorphisation can thus confer the advantages of the amorphous form, such as higher apparent solubility and faster dissolution rate, without the disadvantage of its physical instability. Copyright © 2015 Elsevier B.V. All rights reserved.
Do conventional monitoring practices indicate in situ air sparging performance?
International Nuclear Information System (INIS)
Johnson, P.C.
1995-01-01
Short-term pilot tests play a key role in the selection and design of in situ air sparging systems. Most pilot tests are less than 24 h in duration and consist of monitoring changes in dissolved oxygen, water levels in wells, soil gas pressures, and soil gas contaminant concentrations while air is injected into the aquifer. These parameters are assumed to be indicators of air sparging feasibility and performance, and are also used in the design of full-scale systems. In this work the authors assess the validity of this critical assumption. Data are presented from a study site where a typical pilot-scale short-term test was conducted, followed by continued operation of a full-scale system for 110 days. Conventional sampling practices were augmented with more discrete and detailed assessment methods. In addition, a tracer gas was used to better understand air distributions, vapor flow paths, and vapor recovery efficiency. The data illustrate that conclusions regarding the performance and applicability of air sparging at the study site vary significantly depending on the monitoring approach used. There was no clear correlation between short-term pilot-test data and extended system performance when using data collected only from conventional groundwater monitoring wells. Attention is focused on petroleum hydrocarbons
Scalability of Several Asynchronous Many-Task Models for In Situ Statistical Analysis.
Energy Technology Data Exchange (ETDEWEB)
Pebay, Philippe Pierre [Sandia National Lab. (SNL-CA), Livermore, CA (United States); Bennett, Janine Camille [Sandia National Lab. (SNL-CA), Livermore, CA (United States); Kolla, Hemanth [Sandia National Lab. (SNL-CA), Livermore, CA (United States); Borghesi, Giulio [Sandia National Lab. (SNL-CA), Livermore, CA (United States)
2017-05-01
This report is a sequel to [PB16], in which we provided a first progress report on research and development towards a scalable, asynchronous many-task, in situ statistical analysis engine using the Legion runtime system. This earlier work included a prototype implementation of a proposed solution, using a proxy mini-application as a surrogate for a full-scale scientific simulation code. The first scalability studies were conducted with the above on modestly-sized experimental clusters. In contrast, in the current work we have integrated our in situ analysis engines with a full-size scientific application (S3D, using the Legion-SPMD model), and have conducted nu- merical tests on the largest computational platform currently available for DOE science ap- plications. We also provide details regarding the design and development of a light-weight asynchronous collectives library. We describe how this library is utilized within our SPMD- Legion S3D workflow, and compare the data aggregation technique deployed herein to the approach taken within our previous work.
Energy Technology Data Exchange (ETDEWEB)
Samsahl, K
1961-06-15
A detailed ion-exchange procedure for the separation of chemical elements in eight groups suitable for subsequent gamma spectrometric analysis is described. The method has been in use for gamma spectrometry of some inorganic - but mostly organic - samples for one year. The separation time for inorganic samples, is usually about 1.5 hours and for organic samples as least 2 hours. One man can separate and count three samples per day. In comparative measurements of short-lived isotopes in biological material 10-12 elements can be analysed thus making possible 30 - 35 determinations per day for one man.
[Study of the phase transformation of TiO2 with in-situ XRD in different gas].
Ma, Li-Jing; Guo, Lie-Jin
2011-04-01
TiO2 sample was prepared by sol-gel method from chloride titanium. The phase transformation of the prepared TiO2 sample was studied by in-situ XRD and normal XRD in different gas. The experimental results showed that the phase transformation temperatures of TiO2 were different under in-situ or normal XRD in different kinds of gas. The transformation of amorphous TiO2 to anatase was controlled by kinetics before 500 degrees C. In-situ XRD showed that the growth of anatase was inhibited, but the transformation of anatase to rutile was accelerated under inactive nitrogen in contrast to air. Also better crystal was obtained under hydrogen than in argon. These all showed that external oxygen might accelerate the growth of TiO2, but reduced gas might partly counteract the negative influence of lack of external oxygen. The mechanism of phase transformation of TiO2 was studied by in-situ XRD in order to control the structure in situ.
E-detailing: information technology applied to pharmaceutical detailing.
Montoya, Isaac D
2008-11-01
E-detailing can be best described as the use of information technology in the field of pharmaceutical detailing. It is becoming highly popular among pharmaceutical companies because it maximizes the time of the sales force, cuts down the cost of detailing and increases physician prescribing. Thus, the application of information technology is proving to be beneficial to both physicians and pharmaceutical companies. When e-detailing was introduced in 1996, it was limited to the US; however, numerous other countries soon adopted this novel approach to detailing and now it is popular in many developed nations. The objective of this paper is to demonstrate the rapid growth of e-detailing in the field of pharmaceutical marketing. A review of e-detailing literature was conducted in addition to personal conversations with physicians. E-detailing has the potential to reduce marketing costs, increase accessibility to physicians and offer many of the advantages of face-to-face detailing. E-detailing is gaining acceptance among physicians because they can access the information of a pharmaceutical product at their own time and convenience. However, the drug safety aspect of e-detailing has not been examined and e-detailing remains a supplement to traditional detailing and is not yet a replacement to it.
Valentin, Jan B; Andreetta, Christian; Boomsma, Wouter; Bottaro, Sandro; Ferkinghoff-Borg, Jesper; Frellsen, Jes; Mardia, Kanti V; Tian, Pengfei; Hamelryck, Thomas
2014-02-01
We propose a method to formulate probabilistic models of protein structure in atomic detail, for a given amino acid sequence, based on Bayesian principles, while retaining a close link to physics. We start from two previously developed probabilistic models of protein structure on a local length scale, which concern the dihedral angles in main chain and side chains, respectively. Conceptually, this constitutes a probabilistic and continuous alternative to the use of discrete fragment and rotamer libraries. The local model is combined with a nonlocal model that involves a small number of energy terms according to a physical force field, and some information on the overall secondary structure content. In this initial study we focus on the formulation of the joint model and the evaluation of the use of an energy vector as a descriptor of a protein's nonlocal structure; hence, we derive the parameters of the nonlocal model from the native structure without loss of generality. The local and nonlocal models are combined using the reference ratio method, which is a well-justified probabilistic construction. For evaluation, we use the resulting joint models to predict the structure of four proteins. The results indicate that the proposed method and the probabilistic models show considerable promise for probabilistic protein structure prediction and related applications. Copyright © 2013 Wiley Periodicals, Inc.
In-situ testing of HEPA filters in the nuclear Karlsruhe filter system
International Nuclear Information System (INIS)
Ohlmeyer, M.; Stotz, W.
1977-01-01
Nuclear plant operators and filter manufacturers are endeavouring to improve environmental protection by intensifying process control and/or improving filter quality. In-situ testing is an important element in these efforts since it represents a direct means of checking the success or otherwise of a particular development. The arrangements for in-situ testing should satisfy the following minimum requirements: the staff should not be exposed to risk during the test; the test method should be objective and reproducible as well as being as sensitive as possible; the test method should permit detection of individual leaks in the filter system so that they can be remedied as efficiently as possible; the test equipment should not necessitate modifications to the extract systems or plant construction; the test should be simple and capable of being carried out with a minimum of effort and equipment. GfK has developed the 'Nuclear-Karlsruhe' filter housing in accordance with these principles. This housing permits in-situ testing similar to the DIN 24184 visual oil-fog test or the DOP test. External visual checks on the general condition of the filter is also possible. A safe system of filter changing with a specially designed plastic bag attachment at an accessible height considerably increases the degree of protection of operating personnel
Mapping of depleted uranium with in situ spectrometry and soil samples
International Nuclear Information System (INIS)
Shebell, P.; Reginatto, M.; Monetti, M.; Faller, S.; Davis, L.
1999-01-01
Depleted uranium (DU) has been developed in the past two decades as a highly effective material for armor penetrating rounds and vehicle shielding. There is now a growing interest in the defense community to determine the presence and extent of DU contamination quickly and with a minimum amount of intrusive sampling. We report on a new approach using deconvolution techniques to quantitatively map DU contamination in surface soil. This approach combines data from soil samples with data from in situ gamma-ray spectrometry measurements to produce an accurate and detailed map of DU contamination. Results of a field survey at the Aberdeen Proving Ground are presented. (author)
In situ polymerization of monomers for polyphenylquinoxaline/graphite fiber composites
Serafini, T. T.; Delvigs, P.; Vannucci, R. D.
1974-01-01
Methods currently used to prepare fiber reinforced, high temperature resistant polyphenylquinoxaline (PPQ) composites employ extremely viscous, low solids content solutions of high molecular weight PPQ polymers. An improved approach, described in this report, consists of impregnating the fiber with a solution of the appropriate monomers instead of a solution of previously synthesized high molecular weight polymer. Polymerization of the monomers occurs in situ on the fiber during the solvent removal and curing stages. The in situ polymerization approach greatly simplifies the fabrication of PPQ graphite fiber composites. The use of low viscosity monomeric type solutions facilitates fiber wetting, permits a high solids content, and eliminates the need for prior polymer synthesis.
Jepsen, Tue Heesgaard; Kristensen, Jesper Langgaard
2014-10-03
We report an improved method for in situ generation of the Ohira-Bestmann reagent. Using the recently reported bench-stable imidazole-1-sulfonyl azide as diazotransfer reagent, this new method represents a scalable and convenient approach for the transformation of aldehydes into terminal alkynes. The method features an easier workup compared to the existing in situ protocol due to increased aqueous solubility of waste products.
Energy Technology Data Exchange (ETDEWEB)
Waugh, C. J., E-mail: cjwaugh@mit.edu; Zylstra, A. B.; Frenje, J. A.; Séguin, F. H.; Petrasso, R. D. [Plasma Science and Fusion Center, MIT, Cambridge, Massachusetts 02139 (United States); Rosenberg, M. J.; Glebov, V. Yu.; Sangster, T. C.; Stoeckl, C. [Laboratory for Laser Energetics, Rochester, New York 14623 (United States)
2015-05-15
Neutron time of flight (nTOF) detectors are used routinely to measure the absolute DD neutron yield at OMEGA. To check the DD yield calibration of these detectors, originally calibrated using indium activation systems, which in turn were cross-calibrated to NOVA nTOF detectors in the early 1990s, a direct in situ calibration method using CR-39 range filter proton detectors has been successfully developed. By measuring DD neutron and proton yields from a series of exploding pusher implosions at OMEGA, a yield calibration coefficient of 1.09 ± 0.02 (relative to the previous coefficient) was determined for the 3m nTOF detector. In addition, comparison of these and other shots indicates that significant reduction in charged particle flux anisotropies is achieved when bang time occurs significantly (on the order of 500 ps) after the trailing edge of the laser pulse. This is an important observation as the main source of the yield calibration error is due to particle anisotropies caused by field effects. The results indicate that the CR-39-nTOF in situ calibration method can serve as a valuable technique for calibrating and reducing the uncertainty in the DD absolute yield calibration of nTOF detector systems on OMEGA, the National Ignition Facility, and laser megajoule.
Waugh, C J; Rosenberg, M J; Zylstra, A B; Frenje, J A; Séguin, F H; Petrasso, R D; Glebov, V Yu; Sangster, T C; Stoeckl, C
2015-05-01
Neutron time of flight (nTOF) detectors are used routinely to measure the absolute DD neutron yield at OMEGA. To check the DD yield calibration of these detectors, originally calibrated using indium activation systems, which in turn were cross-calibrated to NOVA nTOF detectors in the early 1990s, a direct in situ calibration method using CR-39 range filter proton detectors has been successfully developed. By measuring DD neutron and proton yields from a series of exploding pusher implosions at OMEGA, a yield calibration coefficient of 1.09 ± 0.02 (relative to the previous coefficient) was determined for the 3m nTOF detector. In addition, comparison of these and other shots indicates that significant reduction in charged particle flux anisotropies is achieved when bang time occurs significantly (on the order of 500 ps) after the trailing edge of the laser pulse. This is an important observation as the main source of the yield calibration error is due to particle anisotropies caused by field effects. The results indicate that the CR-39-nTOF in situ calibration method can serve as a valuable technique for calibrating and reducing the uncertainty in the DD absolute yield calibration of nTOF detector systems on OMEGA, the National Ignition Facility, and laser megajoule.
International Nuclear Information System (INIS)
Waugh, C. J.; Zylstra, A. B.; Frenje, J. A.; Séguin, F. H.; Petrasso, R. D.; Rosenberg, M. J.; Glebov, V. Yu.; Sangster, T. C.; Stoeckl, C.
2015-01-01
Neutron time of flight (nTOF) detectors are used routinely to measure the absolute DD neutron yield at OMEGA. To check the DD yield calibration of these detectors, originally calibrated using indium activation systems, which in turn were cross-calibrated to NOVA nTOF detectors in the early 1990s, a direct in situ calibration method using CR-39 range filter proton detectors has been successfully developed. By measuring DD neutron and proton yields from a series of exploding pusher implosions at OMEGA, a yield calibration coefficient of 1.09 ± 0.02 (relative to the previous coefficient) was determined for the 3m nTOF detector. In addition, comparison of these and other shots indicates that significant reduction in charged particle flux anisotropies is achieved when bang time occurs significantly (on the order of 500 ps) after the trailing edge of the laser pulse. This is an important observation as the main source of the yield calibration error is due to particle anisotropies caused by field effects. The results indicate that the CR-39-nTOF in situ calibration method can serve as a valuable technique for calibrating and reducing the uncertainty in the DD absolute yield calibration of nTOF detector systems on OMEGA, the National Ignition Facility, and laser megajoule
Mardekian, Stacey K; Solomides, Charalambos C; Gong, Jerald Z; Peiper, Stephen C; Wang, Zi-Xuan; Bajaj, Renu
2015-11-01
Atypical lipomatous tumor/well-differentiated liposarcoma (ALT-WDLPS) and dedifferentiated liposarcoma (DDLPS) are characterized cytogenetically by a 12q13-15 amplification involving the mouse double minute 2 (MDM2) oncogene. Fluorescence in situ hybridization (FISH) is used frequently to detect this amplification and aid with the diagnosis of these entities, which is difficult by morphology alone. Recently, bright-field in situ hybridization techniques such as chromogenic in situ hybridization (CISH) have been introduced for the determination of MDM2 amplification status. The present study compared the results of FISH and CISH for detecting MDM2 amplification in 41 cases of adipocytic tumors. Amplification was defined in both techniques as a MDM2/CEN12 ratio of 2 or greater. Eleven cases showed amplification with both FISH and CISH, and 26 cases showed no amplification with both methods. Two cases had discordant results between CISH and FISH, and two cases were not interpretable by CISH. CISH is advantageous for allowing pathologists to evaluate the histologic and molecular alterations occurring simultaneously in a specimen. Moreover, CISH is found to be more cost- and time-efficient when used with automation, and the signals do not quench over time. CISH technique is a reliable alternative to FISH in the evaluation of adipocytic tumors for MDM2 amplification. © 2014 Wiley Periodicals, Inc.
Comparison on in situ and laboratory gamma-ray spectroscopy of ...
African Journals Online (AJOL)
In situ - ray spectroscopic method of measeruments, using the calibration factor by Zombori et al, and laboratory method for soil samples were carried out in Ibadan, SW Nigeria. The average specific activities of 40K, 238U and 232Th in the soil were 299.0 16.5 Bqkg-1, 40.0 5.8 Bqkg-1 and 95.0 7.8 Bqkg-1, respectively, by in ...
Energy Technology Data Exchange (ETDEWEB)
Berry-Spark, K L; Barker, J F; Mayfield, C I
1990-12-31
In-situ methods for treatment of waste sludges hold great promise for efficient remediation of sludge at waste disposal sites, such as the diverse and complex sludges from the O.E. MacDougall site near Brockville, Ontario. This report presents results of laboratory testing of natural bioremediation techniques using six representative soils and sludges obtained from the MacDougall site. Four of six samples contained concentrations of hydrocarbons typical of petroleum products and solvents. The report includes descriptions of the characterisation of the organic chemistry and microbial populations of the soils, as well as of the experiments conducted under aerobic, methane oxidising, anaerobic-denitrifying, sulphate reducing, and methanogenic conditions.
Computer modeling of fluid flow and combustion in the ISV [In Situ Vitrification] confinement hood
International Nuclear Information System (INIS)
Johnson, R.W.; Paik, S.
1990-09-01
Safety and suitability objectives for the application of the In Situ Vitrification (ISV) technology at the INEL require that the physical processes involved in ISVV be modeled to determine their operational behavior. The mathematical models that have been determined to address the modeling needs adequately for the ISV analysis package are detailed elsewhere. The present report is concerned with the models required for simulating the reacting flow that occurs in the ISV confinement hood. An experimental code named COYOTE has been secured that appears adequate to model the combustion in the confinement hood. The COYOTE code is a two-dimensional, transient, compressible, Eulerian, gas dynamics code for modeling reactive flows. It recognizes nonuniform Cartesian and cylindrical geometry and is based on the ICE (Implicit Continuous-fluid Eulerian) family of solution methods. It includes models for chemical reactions based on chemical kinetics as well as equilibrium chemistry. The mathematical models contained in COYOTE, their discrete analogs, the solution procedure, code structure and some test problems are presented in the report. 12 refs., 17 figs., 6 tabs
SEMANTIC INDEXING OF TERRASAR-X AND IN SITU DATA FOR URBAN ANALYTICS
Espinoza Molina, D.; Alonso, K.; Datcu, M.
2015-01-01
This paper presents the semantic indexing of TerraSAR-X images and in situ data. Image processing together with machine learning methods, relevance feedback techniques, and human expertise are used to annotate the image content into a land use land cover catalogue. All the generated information is stored into a geo-database supporting the link between different types of information and the computation of queries and analytics. We used 11 TerraSAR-X scenes over Germany and LUCAS as in situ dat...
In-situ XRD and EDS method study on the oxidation behaviour of Ni-Cu sulphide ore.
Li, Guangshi; Cheng, Hongwei; Xiong, Xiaolu; Lu, Xionggang; Xu, Cong; Lu, Changyuan; Zou, Xingli; Xu, Qian
2017-06-12
The oxidation mechanism of sulfides is the key issue during the sulphide-metallurgy process. In this study, the phase transformation and element migration were clearly demonstrated by in-situ laboratory-based X-ray diffraction (XRD) and energy-dispersive X-ray spectroscopy (EDS), respectively. The reaction sequence and a four-step oxidation mechanism were proposed and identified. The elemental distribution demonstrated that at a low temperature, the Fe atoms diffused outward and the Ni/Cu atoms migrated toward the inner core, whereas the opposite diffusion processes were observed at a higher temperature. Importantly, the unique visual presentation of the oxidation behaviour provided by the combination of in-situ XRD and EDS might be useful for optimising the process parameters to improve the Ni/Cu extraction efficiency during Ni-Cu sulphide metallurgy.
In Situ Mechanical Testing of Nanostructured Bijel Fibers.
Haase, Martin F; Sharifi-Mood, Nima; Lee, Daeyeon; Stebe, Kathleen J
2016-06-28
Bijels are a class of soft materials with potential for application in diverse areas including healthcare, food, energy, and reaction engineering due to their unique structural, mechanical, and transport properties. To realize their potential, means to fabricate, characterize, and manipulate bijel mechanics are needed. We recently developed a method based on solvent transfer-induced phase separation (STRIPS) that enables continuous fabrication of hierarchically structured bijel fibers from a broad array of constituent fluids and nanoparticles using a microfluidic platform. Here, we introduce an in situ technique to characterize bijel fiber mechanics at initial and final stages of the formation process within a microfluidics device. By manipulation of the hydrodynamic stresses applied to the fiber, the fiber is placed under tension until it breaks into segments. Analysis of the stress field allows fracture strength to be inferred; fracture strengths can be as high as several thousand Pa, depending on nanoparticle content. These findings broaden the potential for the use of STRIPS bijels in applications with different mechanical demands. Moreover, our in situ mechanical characterization method could potentially enable determination of properties of other soft fibrous materials made of hydrogels, capillary suspensions, colloidal gels, or high internal phase emulsions.
In situ SU-8 silver nanocomposites
Directory of Open Access Journals (Sweden)
Søren V. Fischer
2015-07-01
Full Text Available Nanocomposite materials containing metal nanoparticles are of considerable interest in photonics and optoelectronics applications. However, device fabrication of such materials always encounters the challenge of incorporation of preformed nanoparticles into photoresist materials. As a solution to this problem, an easy new method of fabricating silver nanocomposites by an in situ reduction of precursors within the epoxy-based photoresist SU-8 has been developed. AgNO3 dissolved in acetonitrile and mixed with the epoxy-based photoresist SU-8 forms silver nanoparticles primarily during the pre- and post-exposure soft bake steps at 95 °C. A further high-temperature treatment at 300 °C resulted in the formation of densely homogeneously distributed silver nanoparticles in the photoresist matrix. No particle growth or agglomeration of nanoparticles is observed at this point. The reported new in situ silver nanocomposite materials can be spin coated as homogeneous thin films and structured by using UV lithography. A resolution of 5 µm is achieved in the lithographic process. The UV exposure time is found to be independent of the nanoparticle concentration. The fabricated silver nanocomposites exhibit high plasmonic responses suitable for the development of new optoelectronic and optical sensing devices.
Munasir; Luvita, N. R. D.; Kusumawati, D. H.; Putri, N. P.; Triwikantoro; Supardi, Z. A. I.
2018-03-01
Silica which is synthesized from natural materials such as Bancar Tuban’s sand composited with Polyaniline (PANi), where the silica used are silica has an amorphous phase and cristobalite phase. In this research, the composite method used is in- situ polymerization, which is silica entered during the fabrication of PANi, then automatically silica will be substitute into the chain bonding of PANi. The aim of this research is to find out the results of a composite process using in-situ methods as well as differences in the morphology of PANi/a- SiO2 and PANi/c-SiO2. For the characterization of samples tested in the form of FTIR to determine the functional groups of the composite and SEM to determine the morphology of the sample. From the test results of FTIR are known composite possibility has occurred because there are several functional groups belonging to silica also functional groups belonging polyaniline, functional group that’s happened in wave numbers were almost identical between PANi/a-SiO2 and PANi/c-SiO2, but there are little differences were seen in the form of a graph generated from the peak and intensity that occurred charts for PANi/c-SiO2 has peak more pointed or sharp compared to PANi/a-SiO2 because that bond of crystal is strong, stiff and has a larger particle size than the amorphous composite. Then from the data of SEM seen clearly their morphological differences between PANi/a-SiO2 and PANi/c-SiO2 where polyaniline is composited with amorphous silica will have a fault that is not uniform or irregular different from PANi/c -SiO2 has a regular fault and this is corresponding with the nature of the typical structure of amorphous and crystalline.
Design Games for In-Situ Design
DEFF Research Database (Denmark)
Kristiansen, Erik
2013-01-01
The mobile culture has spawned a host of context-based products, like location-based and tag-based applications. This presents a new challenge for the designer. There is a need of design methods that acknowledge the context and allows it to influence the design ideas. This article focuses...... on a design problem where an in-situ design practice may further the early design process: the case of designing a pervasive game. Pervasive games are computer games, played using the city as a game board and often using mobile phones with GPS. Some contextual design methods exist, but we propose an approach...... sitestorming, is based on a game using Situationistic individual exploration of the site and different types of game cards, followed by a joint evaluation of the generated ideas. A series of evaluations showed that the designers found the method enjoyable to use, that the method motivated idea generation...
Energy Technology Data Exchange (ETDEWEB)
Cenoz, S.; Hernandez, J.; Gangutia, N.
2004-07-01
This work describes the use of Low-Grade MgO as a stabiliser agent for polluted soil reclaim. Low-Grade MgO may be an economically feasible alternative in the stabilisation of heavy metals from heavily contaminated soils. The effectiveness of Low-Grade MgO has been studied in three ex-situ stabilisation of heavily polluted soils contaminated by the flue-dust of pyrite roasting. LG-MgO provides an alkali reservoir guaranteeing long-term stabilisation without varying the pH conditions. The success of the ex-situ stabilisation was corroborated with the analysis of heavy metals in the leachates collected from the landfill o ver a long period of time. The study also includes the results obtained in an in-situ pilot scale stabilisation of contaminated soil. (Author) 17 refs.
Sintering of oxide-supported Pt and Pd nanoparticles in air studied by in situ TEM
DEFF Research Database (Denmark)
Simonsen, Søren Bredmose
This thesis presents a fundamental study of the sintering of supported nanoparticles in relation to diesel oxidation catalysts. The sintering of supported nanoparticles is an important challenge in relation to this catalyst, as well as many other catalyst systems, and a fundamental understanding...... of Pt, Pd and bimetallic Pt-Pd nanoparticles supported on a flat and homogeneous Al2O3 or SiO2 surface. By using in situ TEM on the planar model catalysts it was possible to directly monitor the detailed dynamical changes of the individual nanoparticles during exposure to oxidizing conditions...
DEFF Research Database (Denmark)
Kjellström, Sven; Jensen, Ole Nørregaard
2003-01-01
A novel liquid-liquid extraction (LLE) procedure was investigated for preparation of peptide and protein samples for matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS). LLE using ethyl acetate as the water-immiscible organic solvent enabled segregation of hydrophobic...... matrix to the organic solvent enhanced the efficiency of the LLE-MALDI MS method for analysis of hydrophobic peptides and proteins. LLE-MALDI MS enabled the detection of the hydrophobic membrane protein bacteriorhodopsin as a component in a simple protein mixture. Peptide mixtures containing...... phosphorylated, glycosylated, or acylated peptides were successfully separated and analyzed by the in situ LLE-MALDI MS technique and demonstrate the potential of this method for enhanced separation and structural analysis of posttranslationally modified peptides in proteomics research....
In situ generation of the Ohira-Bestmann Reagent from stable sulfonyl azide
DEFF Research Database (Denmark)
Jepsen, Tue Heesgaard; Kristensen, Jesper Langgaard
2014-01-01
We report an improved method for in situ generation of the Ohira-Bestmann reagent. Using the recently reported bench stable imidazole-1-sulfonyl azide as diazotransfer reagent, this new method represents a safe and scalable approach for the transformation of aldehydes into terminal alkynes...
International Nuclear Information System (INIS)
Siegrist, R.L.; Gates, D.D.; West, O.R.; Liang, L.; Donaldson, T.L.; Webb, O.F.; Corder, S.L.; Dickerson, K.S.
1994-06-01
The U.S. Department of Energy (DOE) In Situ Remediation Integrated Program (ISR IP) was established in June 1991 to facilitate the development and implementation of in situ remediation technologies for environmental restoration within the DOE complex. Within the ISR IP, four subareas of research have been identified: (1) in situ containment, (2) in situ physical/chemical treatment (ISPCT), (3) in situ bioremediation, and (4) subsurface manipulation/electrokinetics. Although set out as individual focus areas, these four are interrelated, and successful developments in one will often necessitate successful developments in another. In situ remediation technologies are increasingly being sought for environmental restoration due to the potential advantages that in situ technologies can offer as opposed to more traditional ex situ technologies. These advantages include limited site disruption, lower cost, reduced worker exposure, and treatment at depth under structures. While in situ remediation technologies can offer great advantages, many technology gaps exist in their application. This document presents an overview of ISPCT technologies and describes their applicability to DOE-complex needs, their development status, and relevant ongoing research. It also highlights research needs that the ISR IP should consider when making funding decisions
Role of in-situ simulation for training in healthcare: opportunities and challenges.
Kurup, Viji; Matei, Veronica; Ray, Jessica
2017-12-01
Simulation has now been acknowledged as an important part of training in healthcare, and most academic hospitals have a dedicated simulation center. In-situ simulation occurs in patient care units with scenarios involving healthcare professionals in their actual working environment. The purpose of this review is to describe the process of putting together the components of in-situ simulation for training programs and to review outcomes studied, and challenges with this approach. In-situ simulation has been used to 'test-drive' new centers, train personnel in new procedures in existing centers, for recertification training and to uncover latent threats in clinical care areas. It has also emerged as an attractive alternative to traditional simulations for institutions that do not have their own simulation center. In-situ simulation can be used to improve reliability and safety especially in areas of high risk, and in high-stress environments. It is also a reasonable and attractive alternative for programs that want to conduct interdisciplinary simulations for their trainees and faculty, and for those who do not have access to a fully functional simulation center. Further research needs to be done in assessing effectiveness of training using this method and the effect of such training on clinical outcomes.
Martin, P. G.; Griffiths, I.; Jones, C. P.; Stitt, C. A.; Davies-Milner, M.; Mosselmans, J. F. W.; Yamashiki, Y.; Richards, D. A.; Scott, T. B.
2016-03-01
Traditional methods to locate and subsequently study radioactive fallout particles have focused heavily on autoradiography coupled with in-situ analytical techniques. Presented here is the application of a Variable Pressure Scanning Electron Microscope with both backscattered electron and energy dispersive spectroscopy detectors, along with a micromanipulator setup and electron-hardening adhesive to isolate and remove individual particles before synchrotron radiation analysis. This system allows for a greater range of new and existing analytical techniques, at increased detail and speed, to be applied to the material. Using this method, it was possible to erform detailed energy dispersive spectroscopy and synchrotron radiation characterisation of material likely ejected from the Fukushima Daiichi Nuclear Power Plant found within a sediment sample collected from the edge of the 30 km exclusion zone. Particulate material sub-micron in maximum dimension examined during this work via energy dispersive spectroscopy was observed to contain uranium at levels between 19.68 and 28.35 weight percent, with the application of synchrotron radiation spectroscopy confirming its presence as a major constituent. With great effort and cost being devoted to the remediation of significant areas of eastern Japan affected by the incident, it is crucial to gain the greatest possible understanding of the nature of this contamination in order to inform the most appropriate clean-up response.
Junfeng Feng; Chung-yun Hse; Zhongzhi Yang; Kui Wang; Jianchun Jiang; Junming Xu
2017-01-01
The objective of this study was to find an effective method for converting renewable biomass-derived phenolic compounds into hydrocarbons bio-fuel via in situ catalytic hydrodeoxygenation. The in situ hydrodeoxygenation of biomass-derived phenolic compounds was carried out in methanol-water solvent over bifunctional catalysts of Raney Ni and HZSM-5 or H-Beta. In the in...
White, P C; Munro, C H; Smith, W E
1996-06-01
An in situ surface enhanced resonance Raman scattering (SERRS) procedure is described for the analysis of a reactive dye covalently bound to a single strand of a cotton fibre. This procedure can be completed in 5 h, whereas an alternative enzyme digestion method takes approximately 21 h. These two fibre preparation methods give similar spectra from picogram quantities of dye present on a 2-5 mm length of fibre. The in situ nature of the analysis and the small sample size make this method particularly suitable for forensic applications.
Evaluation of In vitro and In vivo Performance of Granisetron In situ ...
African Journals Online (AJOL)
Purpose: To investigate the effect of various solvent systems and gamma irradiation on the in vitro and in vivo performance of granisetron HCl injectable phase-sensitive in situ forming implants (ISFIs). Methods: ISFIs were prepared by mixing and sterilized by gamma irradiation. Effect of solvent system was studied.
Coalescence and compression in centrifuged emulsions studied with in situ optical microscopy
Krebs, T.; Ershov, D.S.; Schroën, C.G.P.H.; Boom, R.M.
2013-01-01
We report an experimental method to investigate droplet dynamics in centrifuged emulsions and its application to study droplet compression and coalescence. The experimental setup permits in situ monitoring of an ensemble of droplets in a centrifuged monolayer of monodisperse emulsion droplets using
Energy Technology Data Exchange (ETDEWEB)
Chelliah, Nagaraj M., E-mail: cmnraj.7@gmail.com [Department of Mechanical Engineering, Indian Institute of Technology Ropar, Rupnagar 140001, Punjab (India); Singh, Harpreet, E-mail: harpreetsingh@iitrpr.ac.in [Department of Mechanical Engineering, Indian Institute of Technology Ropar, Rupnagar 140001, Punjab (India); Surappa, M.K., E-mail: mirle@materials.iisc.ac.in [Department of Materials Engineering, Indian Institute of Science, Bengaluru 560012, Karnataka (India)
2017-06-15
In-situ magnesium matrix composites with three different matrix materials (including Mg, AZ91 and AE44 Mg-alloys) were fabricated by injecting cross-linked polymer directly into the molten Mg/Mg-alloys, and having it convert to the 2.5 vol% SiCNO ceramic phase using liquid stir-casting method. In-situ chemical reaction took place within the molten slurry tending to produce 42 and 18 vol% Mg{sub 2}Si crystals in Mg and AE44 matrix composites, respectively but not in AZ91 matrix composite. Microstructural evolution of Mg{sub 2}Si crystals was discussed on the basis of availability of heterogeneous nucleation sites and amount of Al-atoms in the molten slurry. The observed micro-hardness and yield strengths are enhanced by factor of four to three as compared to their unreinforced counterparts, and Taylor strengthening was found to be the predominant strengthening mechanism in magnesium and AE44 matrix composites. Summation model predicted the yield strengths of the fabricated composites more preciously when compared to Zhang and Chen, and modified Clyne models. - Highlights: • In-situ magnesium composites were fabricated using liquid stir-casting method. • In-situ pyrolysis of cross-linked polymer has been utilized to obtain ceramic phases. • Mg{sub 2}Si crystals were formed in magnesium and AE44 matrix composites but not in AZ91 matrix composites. • The variation in size and morphology of Mg{sub 2}Si crystals with matrix materials are discussed. • Strengthening mechanisms in in-situ composites are analyzed and discussed.
International Nuclear Information System (INIS)
Fayek, M.; Harrison, T.M.; Grove, M.; Coath, C.D.
2000-01-01
The authors present a rapid and accurate technique for making in situ U-Pb isotopic measurements of uranium oxide minerals that utilizes both electron and ion microprobes. U and Pb concentrations are determined using an electron microprobe, whereas the isotopic composition of Pb for the same area is measured using a high-resolution ion microprobe. The advantages of this approach are: mineral separation and chemical digestion are unnecessary; homogeneous uranium oxide standards, which are difficult to obtain, are not required; and precise and accurate U-Pb ages on ∼10 microm spots can be obtained in a matter of hours. The authors have applied their method to study the distribution of U-Pb ages in complexly intergrown uranium oxides from the unconformity-type Cigar Lake uranium deposit, Saskatchewan, Canada. In situ U-Pb results from early formed uraninite define a well-correlated array on concordia with upper and lower intercepts of 1,467 ± 63 Ma and 443 ± 96 Ma (±1σ), respectively. The 1,467 Ma age is interpreted as the minimum age of mineralization and is consistent with the age of clay-mineral alteration (approximately1477 Ma) and magnetization of diagenetic hematite (1,650 to 1,450 Ma) that is associated with these unconformity-type uranium deposits and early diagenesis of the Athabasca Basin sediments. In situ U-Pb isotopic analysis of uraninite and coffinite can document the Pb*/U heterogeneities that can occur on a scale of 15 to 30 microm, thus providing relatively accurate information regarding the timing of fluid interactions associated with the evolution of these deposits
Gallegos, Tanya J.; Bern, Carleton R.; Birdwell, Justin E.; Haines, Seth; Engle, Mark A.
2015-01-01
Global trends toward developing new energy resources from lower grade, larger tonnage deposits that are not generally accessible using “conventional” extraction methods involve variations of subsurface in situ extraction techniques including in situ oil-shale retorting, hydraulic fracturing of petroleum reservoirs, and in situ recovery (ISR) of uranium. Although these methods are economically feasible and perhaps result in a smaller above-ground land-use footprint, there remain uncertainties regarding potential subsurface impacts to groundwater. This chapter provides an overview of the role of water in these technologies and the opportunities and challenges for water reuse and recycling.
Loading of deuterium and helium by Pilot-PSI plasma and their detection by in-situ LIBS
Piip, K.; van der Meiden, H. J.; Bystrov, K.; Hämarik, L.; Karhunen, J.; Aints, M.; Laan, M.; Paris, P.; Seemen, H.; Hakola, A.; Brezinsek, S.
2017-01-01
Laser-induced breakdown spectroscopy (LIBS) is a promising method for quantifying the fuel content of the plasma-facing components of ITER both in between plasma discharges (in-situ) and after maintenance operations. The aim of the present study is to test the applicability of in-situ LIBS for
Yang, Jianhua; Lu, Wenbo; Chen, Ming; Yan, Peng; Zhou, Chuangbing
2013-07-01
During deep rock mass excavation with the method of drill and blast, accompanying the secession of rock fragments and the formation of a new free surface, in situ stress on this boundary is suddenly released within several milliseconds, which is termed the transient release of in situ stress. In this process, enormous strain energy around the excavation face is instantly released in the form of kinetic energy and it inevitably induces microseismic events in surrounding rock masses. Thus, blasting excavation-induced microseismic vibrations in high-stress rock masses are attributed to the combined action of explosion and the transient release of in situ stress. The intensity of stress release-induced microseisms, which depends mainly on the magnitude of the in situ stress and the dimension of the excavation face, is comparable to that of explosion-induced vibrations. With the methods of time-energy density analysis, amplitude spectrum analysis, and finite impulse response (FIR) digital filter, microseismic vibrations induced by the transient release of in situ stress were identified and separated from recorded microseismic signals during a blast of deep rock masses in the Pubugou Hydropower Station. The results show that the low-frequency component in the microseismic records results mainly from the transient release of in situ stress, while the high-frequency component originates primarily from explosion. In addition, a numerical simulation was conducted to demonstrate the occurrence of microseismic events by the transient release of in situ stress, and the results seem to have confirmed fairly well the separated vibrations from microseismic records.
Wooden houses in detail. Holzhaeuser im Detail
Energy Technology Data Exchange (ETDEWEB)
Ruske, W. (ed.)
1986-01-01
Under the serial title 'Planning and construction of wooden houses', WEKA will publish a number of books of which this is the first. Details of design and construction are presented, e.g.: Details of modern one-family houses; Fundamentals of design and hints for planning of wooden houses and compact wooden structures; Constructional ecology, wood protection, thermal insulation, sound insulation; Modular systems for domestic buildings; The 'bookshelf-type' house at the Berlin International Construction Exhibition (IBA); Experience with do-it-yourself systems. With 439 figs.
DEFF Research Database (Denmark)
Hoshino, Tatsuhiko; Schramm, Andreas
2010-01-01
target site. Finally, the RCA product inside the cells was detected by standard fluorescence in situ hybridization (FISH). The optimized protocol showed high specificity and signal-to-noise ratio but low detection frequency (up to 15% for single-copy genes and up to 43% for the multi-copy 16S rRNA gene...... as Candidatus Accumulibacter phosphatis by combining in situ RCA-FISH with 16S rRNA-targeted FISH. While not suitable for quantification because of its low detection frequency, in situ RCA-FISH will allow to link metabolic potential with 16S rRNA (gene)-based identification of single microbial cells.......). Nevertheless, multiple genes (nirS and nosZ; nirS and the 16S rRNA gene) could be detected simultaneously in P. stutzeri. Environmental application of in situ RCA-FISH was demonstrated on activated sludge by the differential detection of two types of nirS-defined denitrifiers; one of them was identified...
Reliable cost effective technique for in situ ground stress measurements in deep gold mines.
CSIR Research Space (South Africa)
Stacey, TR
1995-07-01
Full Text Available on these requirements, an in situ stress measurement technique which will be practically applicable in the deep gold mines, has been developed conceptually. Referring to the figure on the following page, this method involves: • a borehole-based system, using... level mines have not been developed. 2 This is some of the background to the present SIMRAC research project, the title ofwhich is “Reliable cost effective technique for in-situ ground stress measurements in deep gold mines”. A copy of the research...
In-situ bioremediation via horizontal wells
International Nuclear Information System (INIS)
Hazen, T.C.; Looney, B.B.; Enzien, M.; Franck, M.M.; Fliermans, C.B.; Eddy, C.A.
1993-01-01
This project is designed to demonstrate in situ bioremediation of groundwater and sediment contaminated with chlorinated solvents. Indigenous microorganisms were stimulated to degrade TCE, PCE and their daughter products in situ by addition of nutrients to the contaminated zone. In situ biodegradation is a highly attractive technology for remediation because contaminants are destroyed, not simply moved to another location or immobilized, thus decreasing costs, risks, and time, while increasing efficiency and public and regulatory acceptability. Bioremediation has been found to be among the least costly technologies in applications where it will work (Radian 1989). Subsurface soils and water adjacent to an abandoned process sewer line at the SRS have been found to have elevated levels of TCE (Marine and Bledsoe 1984). This area of subsurface and groundwater contamination is the focus of a current integrated demonstration of new remediation technologies utilizing horizontal wells. Bioremediation has the potential to enhance the performance of in situ air stripping as well as offering stand-alone remediation of this and other contaminated sites (Looney et al. 1991). Horizontal wells could also be used to enhance the recovery of groundwater contaminants for bioreactor conversions from deep or inaccessible areas (e.g., under buildings) and to enhance the distribution of nutrient or microbe additions in an in situ bioremediation
In situ groundwater bioremediation
Energy Technology Data Exchange (ETDEWEB)
Hazen, Terry C.
2009-02-01
In situ groundwater bioremediation of hydrocarbons has been used for more than 40 years. Most strategies involve biostimulation; however, recently bioaugmentation have been used for dehalorespiration. Aquifer and contaminant profiles are critical to determining the feasibility and strategy for in situ groundwater bioremediation. Hydraulic conductivity and redox conditions, including concentrations of terminal electron acceptors are critical to determine the feasibility and strategy for potential bioremediation applications. Conceptual models followed by characterization and subsequent numerical models are critical for efficient and cost effective bioremediation. Critical research needs in this area include better modeling and integration of remediation strategies with natural attenuation.
In-situ study of hydriding kinetics in Pd-based thin film systems
Energy Technology Data Exchange (ETDEWEB)
Delmelle, Renaud; Proost, Joris [Univ. Catholique de Louvain, Louvain-la-Neuve (Belgium). Div. of Materials and Process Engineering
2010-07-01
The hydriding kinetics of Pd thin films has been investigated in detail. The key experimental technique used in this work consists of a high resolution curvature measurement setup, which continuously monitors the reflections of multiple laser beams coming off a cantilevered sample. After mounting the sample inside a vacuum chamber, a H-containing gas mixture is introduced to instantaneously generate a given hydrogen partial pressure (p{sub H2}) inside the chamber. The resulting interaction of H with the Pd layer then leads to a volume expansion of the thin film system. This induces in turn changes in the sample curvature as a result of internal stresses developing in the Pd film during a hydriding cycle. Based on such curvature date obtained in-situ at different p{sub H2}, a two-step model for the kinetics of Pd-hydride formation has been proposed and expressions for the hydrogen adsorption and absorption velocities have been derived. The rate-limiting steps have been identified by studying the p{sub H2}-dependence of these velocities. Furthermore, from our in-situ experimental data, relevant kinetic parameters have been calculated. The effect of dry air exposure of the Pd films on the hydriding kinetics has been considered as well. (orig.)
Application of Genomic In Situ Hybridization in Horticultural Science
Directory of Open Access Journals (Sweden)
Fahad Ramzan
2017-01-01
Full Text Available Molecular cytogenetic techniques, such as in situ hybridization methods, are admirable tools to analyze the genomic structure and function, chromosome constituents, recombination patterns, alien gene introgression, genome evolution, aneuploidy, and polyploidy and also genome constitution visualization and chromosome discrimination from different genomes in allopolyploids of various horticultural crops. Using GISH advancement as multicolor detection is a significant approach to analyze the small and numerous chromosomes in fruit species, for example, Diospyros hybrids. This analytical technique has proved to be the most exact and effective way for hybrid status confirmation and helps remarkably to distinguish donor parental genomes in hybrids such as Clivia, Rhododendron, and Lycoris ornamental hybrids. The genome characterization facilitates in hybrid selection having potential desirable characteristics during the early hybridization breeding, as this technique expedites to detect introgressed sequence chromosomes. This review study epitomizes applications and advancements of genomic in situ hybridization (GISH techniques in horticultural plants.
Next generation in-situ optical Raman sensor for seawater investigations
Kolomijeca, A.; Kwon, Y.-H.; Ahmad, H.; Kronfeldt, H.-D.
2012-04-01
We introduce the next generation of optical sensors based on a combination of surfaced enhanced Raman scattering (SERS) and shifted excitation Raman difference spectroscopy (SERDS) suited for investigations of tiny concentrations of pollutions in the seawater. First field measurements were carried out in the Arctic area which is of global interest since it is more affected by global warming caused climatic changes than any other areas of our planet and it is a recipient for many toxic organic pollutants. A significant long-range atmospheric transport of pollutants to Svalbard is mainly originated from industrialized countries in Europe and North America during the last decades. Therefore, the main interest is to investigate the Arctic water column and also the sediments. Standard chemical methods for water/sediment analysis are extremely accurate but complex and time-consuming. The primary objective of our study was to develop a fast response in-situ optical sensor for easy to use and quick analysis. The system comprises several components: a handheld measurement head containing a 671 nm microsystem diode laser and the Raman optical bench, a laser driver electronics board, a custom-designed miniature spectrometer with an optical resolution of 8 cm-1 and a netbook to control the spectrometer as well as for data evaluation. We introduced for the first time the portable Raman sensor system on an Artic sea-trial during a three week cruise on board of the James Clark Ross research vessel in August 2011. Numerous Raman and SERS measurements followed by SERDS evaluations were taken around locations 78° N and 9° E. Different SERS substrates developed for SERS measurements in sea-water were tested for their capability to detect different substances (PAHs) in the water down to very small (nmol/l) concentrations. Stability tests of the substrates were carried out also for the applicability of our system e.g. on a mooring. Details of the in-situ Raman sensor were presented
Common Fluorescence In Situ Hybridization Applications in Cytology.
Savic, Spasenija; Bubendorf, Lukas
2016-12-01
- Fluorescence in situ hybridization (FISH) is a well-established method for detection of genomic aberrations in diagnostic, prognostic, and predictive marker testing. - To review common applications of FISH in cytology. - The published literature was reviewed. - Cytology is particularly well suited for all kinds of FISH applications, which is highlighted in respiratory tract cytology with an increasing demand for predictive FISH testing in lung cancer. Fluorescence in situ hybridization is the gold standard for detection of predictive anaplastic lymphoma kinase gene (ALK) rearrangements, and the same evaluation criteria as in histology apply to cytology. Several other gene rearrangements, including ROS proto-oncogene 1 receptor tyrosine kinase (ROS1), are becoming clinically important and share the same underlining cytogenetic mechanisms with ALK. MET amplification is one of the most common mechanisms of acquired resistance to epidermal growth factor receptor (EGFR) tyrosine kinase inhibitors and can be targeted by crizotinib. As genomic aberrations are a hallmark of malignant cells, FISH is a valuable objective ancillary diagnostic tool. In urinary tract cytology, atypical urothelial cells equivocal for malignancy are a common diagnostic dilemma and multitarget FISH can help clarify such cells. Diagnosis of malignant mesothelioma remains one of the most challenging fields in effusion cytology, and ancillary FISH is useful in establishing the diagnosis. Fluorescence in situ hybridization is a morphology-based technique, and the prerequisite for reliable FISH results is a targeted evaluation of the cells in question (eg, cancer or atypical cells). Cytopathologists and cytotechnicians should therefore be involved in molecular testing in order to select the best material and to provide their morphologic expertise.