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Sample records for in-class demonstrations active

  1. Using a CBL Unit, a Temperature Sensor, and a Graphing Calculator to Model the Kinetics of Consecutive First-Order Reactions as Safe In-Class Demonstrations

    Science.gov (United States)

    Moore-Russo, Deborah A.; Cortes-Figueroa, Jose E.; Schuman, Michael J.

    2006-01-01

    The use of Calculator-Based Laboratory (CBL) technology, the graphing calculator, and the cooling and heating of water to model the behavior of consecutive first-order reactions is presented, where B is the reactant, I is the intermediate, and P is the product for an in-class demonstration. The activity demonstrates the spontaneous and consecutive…

  2. An Examination of In-Class Physical Activity across Games Classifications

    Science.gov (United States)

    Perlman, Dana J.; Forrest, Greg

    2015-01-01

    The purpose of this study was to examine the in-class physical activity opportunities across game classifications. A total of 221 (male, 100; female, 121) Year 9/10 physical education students were used within this study. Each student was engaged in four sport-based units (target, net/wall, striking/fielding, and invasion). Physical activity data…

  3. Making Waves: Seismic Waves Activities and Demonstrations

    Science.gov (United States)

    Braile, S. J.; Braile, L. W.

    2011-12-01

    The nature and propagation of seismic waves are fundamental concepts necessary for understanding the exploration of Earth's interior structure and properties, plate tectonics, earthquakes, and seismic hazards. Investigating seismic waves is also an engaging approach to learning basic principles of the physics of waves and wave propagation. Several effective educational activities and demonstrations are available for teaching about seismic waves, including the stretching of a spring to demonstrate elasticity; slinky wave propagation activities for compressional, shear, Rayleigh and Love waves; the human wave activity to demonstrate P- and S- waves in solids and liquids; waves in water in a simple wave tank; seismic wave computer animations; simple shake table demonstrations of model building responses to seismic waves to illustrate earthquake damage to structures; processing and analysis of seismograms using free and easy to use software; and seismic wave simulation software for viewing wave propagation in a spherical Earth. The use of multiple methods for teaching about seismic waves is useful because it provides reinforcement of the fundamental concepts, is adaptable to variable classroom situations and diverse learning styles, and allows one or more methods to be used for authentic assessment. The methods described here have been used effectively with a broad range of audiences, including K-12 students and teachers, undergraduate students in introductory geosciences courses, and geosciences majors.

  4. Guidance manual for conducting technology demonstration activities

    International Nuclear Information System (INIS)

    Jolley, R.L.; Morris, M.I.; Singh, S.P.N.

    1991-12-01

    This demonstration guidance manual has been prepared to assist Martin Marietta Energy Systems, Inc. (Energy Systems), staff in conducting demonstrations. It is prepared in checklist style to facilitate its use and assumes that Energy Systems personnel have project management responsibility. In addition to a detailed step-by-step listing of procedural considerations, a general checklist, logic flow diagram, and several examples of necessary plans are included to assist the user in developing an understanding of the many complex activities required to manage technology demonstrations. Demonstrations are pilot-scale applications of often innovative technologies to determine the commercial viability of the technologies to perform their designed function. Demonstrations are generally conducted on well-defined problems for which existing technologies or processes are less than satisfactory in terms of effectiveness, cost, and/or regulatory compliance. Critically important issues in demonstration management include, but are not limited to, such factors as communications with line and matrix management and with the US Department of Energy (DOE) and Energy Systems staff responsible for management oversight, budgetary and schedule requirements, regulatory compliance, and safety

  5. Guidance manual for conducting technology demonstration activities

    Energy Technology Data Exchange (ETDEWEB)

    Jolley, Robert L.; Morris, Michael I.; Singh, Suman P.N.

    1991-12-01

    This demonstration guidance manual has been prepared to assist Martin Marietta Energy Systems, Inc. (Energy Systems), staff in conducting demonstrations. It is prepared in checklist style to facilitate its use and assumes that Energy Systems personnel have project management responsibility. In addition to a detailed step-by-step listing of procedural considerations, a general checklist, logic flow diagram, and several examples of necessary plans are included to assist the user in developing an understanding of the many complex activities required to manage technology demonstrations. Demonstrations are pilot-scale applications of often innovative technologies to determine the commercial viability of the technologies to perform their designed function. Demonstrations are generally conducted on well-defined problems for which existing technologies or processes are less than satisfactory in terms of effectiveness, cost, and/or regulatory compliance. Critically important issues in demonstration management include, but are not limited to, such factors as communications with line and matrix management and with the US Department of Energy (DOE) and Energy Systems staff responsible for management oversight, budgetary and schedule requirements, regulatory compliance, and safety.

  6. Self-efficacy as a mediator of children's achievement motivation and in-class physical activity.

    Science.gov (United States)

    Gao, Zan; Lochbaum, Marc; Podlog, Leslie

    2011-12-01

    The present study was designed to examine the mediating effect of self-efficacy on relations of middle school students' four achievement goals with their perceptions of two motivational climates and in-class physical activity in physical education. The four achievement goals (mastery-approach, mastery-avoidance, performance-approach, and performance-avoidance), perceptions of the motivational climate (mastery- and performance-involving climates), and self-efficacy were measured in a sample of 194 students (93 boys, 101 girls) in a public school. Students' in-class physical activity was assessed using Actical Accelerometers. A series of multiple-regression analyses supported the mediating effect of self-efficacy on the relationships among students' mastery-approach goal, perceived mastery-involving climate, and physical activity.

  7. An Eectromyographic Ccomparison Between the Activities of Temporal and Masseter Muscles in Class III Skeletal

    Directory of Open Access Journals (Sweden)

    T Hossein-Zadeh-Nik

    2002-02-01

    Full Text Available Electromyographic (EMG investigations about the activities of the muscles have been the focus of attention for many years. In the field of orthodontics, investigators, among other things, tried to evaluate correlation between EMG activity, occlusal relationships and craniofacial morphology to analyze the effect of muscular activity, as an etiological factor in malocclusion. The purpose of the present investigation is to analyze the effect of EMG activity of temporal and masseter muscles quantitatively in skeletal class III malocclusion. 26 patients (9 to If years old, with class III malocclusion were selected and their EMG activity of temporal and masseter muscles in rest position, centric occlusion, clenching, mastication and swallowing were compared with 20 normal children at the same age range. Then the statistical correlation between 13 cephalometric parameters and EMG activities were analyzed and then the regression analysis was performed and the results were as follows:1- The mean amplitude of masseter and temporal muscles activity in rest position, centric occlusion, mastication, and clenching in class III samples were greater than normal group (PO.05.2- The mean duration of masseter and temporal muscles activity in rest position and centric occlusion in class III samples were more than normal group (PO.05.3- According to regression analysis, a linear correlation was observed between ANB angle and temporal muscle activity in rest and centric occlusion that was not observed in other cases.The findings of this study showed that difference in temporal muscle activity in class III malocclusion, in comparison with the normal group, is correlated with skeletal morphology of the face, but according to other investigations it is not ture for the masseter muscle.

  8. Implementation and impact of in-class physical activities in a positive mental health perspective

    DEFF Research Database (Denmark)

    Christiansen, Lars Breum Skov; Holt, Anne-Didde; Smedegaard, Søren

    Introduction School physical activity and other activities with the body in focus hold the potential to benefit student’s positive mental health and psychosocial well-being. In-class activities (ICAs) (e.g. energizers, active breaks, brain breaks) can positively influence social connectedness...... intervention components in the RCT-designed ‘Move for well-being in school’ research program. The program comprises 2797 students 9-13 years old on 12 intervention schools and 12 comparison schools. During a full school year ICAs should be delivered two times every day focusing on four different aims: social...... interaction, energizing, coordination and wellness. A mixed methods design was used to evaluate the intervention comprising questionnaires and interviews with teachers and students. The collected data was used to assess the degree of implementation; perception of advantages and pitfalls of ICAs...

  9. Simple Activity Demonstrates Wind Energy Principles

    Science.gov (United States)

    Roman, Harry T.

    2012-01-01

    Wind energy is an exciting and clean energy option often described as the fastest-growing energy system on the planet. With some simple materials, teachers can easily demonstrate its key principles in their classroom. (Contains 1 figure and 2 tables.)

  10. Inverse correlation between promoter strength and excision activity in class 1 integrons.

    Directory of Open Access Journals (Sweden)

    Thomas Jové

    2010-01-01

    Full Text Available Class 1 integrons are widespread genetic elements that allow bacteria to capture and express gene cassettes that are usually promoterless. These integrons play a major role in the dissemination of antibiotic resistance among Gram-negative bacteria. They typically consist of a gene (intI encoding an integrase (that catalyzes the gene cassette movement by site-specific recombination, a recombination site (attI1, and a promoter (Pc responsible for the expression of inserted gene cassettes. The Pc promoter can occasionally be combined with a second promoter designated P2, and several Pc variants with different strengths have been described, although their relative distribution is not known. The Pc promoter in class 1 integrons is located within the intI1 coding sequence. The Pc polymorphism affects the amino acid sequence of IntI1 and the effect of this feature on the integrase recombination activity has not previously been investigated. We therefore conducted an extensive in silico study of class 1 integron sequences in order to assess the distribution of Pc variants. We also measured these promoters' strength by means of transcriptional reporter gene fusion experiments and estimated the excision and integration activities of the different IntI1 variants. We found that there are currently 13 Pc variants, leading to 10 IntI1 variants, that have a highly uneven distribution. There are five main Pc-P2 combinations, corresponding to five promoter strengths, and three main integrases displaying similar integration activity but very different excision efficiency. Promoter strength correlates with integrase excision activity: the weaker the promoter, the stronger the integrase. The tight relationship between the aptitude of class 1 integrons to recombine cassettes and express gene cassettes may be a key to understanding the short-term evolution of integrons. Dissemination of integron-driven drug resistance is therefore more complex than previously thought.

  11. The Intersection between Out-of-class Language Learning Strategies and In-class Activities

    Directory of Open Access Journals (Sweden)

    Noor Saazai Mat Saad

    2013-07-01

    Full Text Available Studies on out-of-class language learning strategies (OCLLSs are usually divorced from the activities in class. Thus, this study addresses the connection of the two entities. The participants in this study were nine international postgraduate students who were undergoing their English language proficiency course in an institution in Malaysia.  Data were gathered through their weekly online postings on Google + and interview during the course. The data from the students were triangulated with the information found in the pro forma of the modules and interview with the lecturers. Atlas ti was used to manage the data.  It was discovered that the type of assessment set by lecturers for the course determined the use of OCLLSs. Findings show that firstly, more OCLLS were used in completing assignments than preparing for quizzes/tests. Thus, from the 3 modules; it was found that students employed more strategies for oral communication and writing rather than for reading.  Secondly, the OCLLSs used could form a 'strategy chain or cluster' for the tasks of preparing for oral presentation and completing writing assignments. Lastly, there was evidence of technology dependency on some of the main OCLLSs.

  12. A qualitative study of how to create supportive environments for the implementation of in-class-activities in middle school

    DEFF Research Database (Denmark)

    Holt, Anne-Didde; Christiansen, Lars Breum Skov; Smedegaard, Søren

    Purpose School physical activity (SPA) holds the potential to benefit children’s psychosocial well-being. PA can positively influence mental health through social connectedness, physical self-perception, and improvements in emotions. For such reasons, in-class-activities (ICA) (e.g. energizers......, active breaks) has been given increased attention in the literature. However, if all students are to benefit from the potential mental health qualities of ICA, the right supportive environment is crucial. The purpose of this study was, on the basis of students and teachers feedback to an ICA...... was important to satisfy different needs of the students, and the focus on social connectedness instead of competition was essential regarding the motivation of the less active students....

  13. Pevonedistat (MLN4924), a First-in-Class NEDD8-activating enzyme inhibitor, in patients with acute myeloid leukaemia and myelodysplastic syndromes: a phase 1 study.

    Science.gov (United States)

    Swords, Ronan T; Erba, Harry P; DeAngelo, Daniel J; Bixby, Dale L; Altman, Jessica K; Maris, Michael; Hua, Zhaowei; Blakemore, Stephen J; Faessel, Hélène; Sedarati, Farhad; Dezube, Bruce J; Giles, Francis J; Medeiros, Bruno C

    2015-05-01

    This trial was conducted to determine the dose-limiting toxicities (DLTs) and maximum tolerated dose (MTD) of the first in class NEDD8-activating enzyme (NAE) inhibitor, pevonedistat, and to investigate pevonedistat pharmacokinetics and pharmacodynamics in patients with acute myeloid leukaemia (AML) and myelodysplastic syndromes (MDS). Pevonedistat was administered via a 60-min intravenous infusion on days 1, 3 and 5 (schedule A, n = 27), or days 1, 4, 8 and 11 (schedule B, n = 26) every 21-days. Dose escalation proceeded using a standard '3 + 3' design. Responses were assessed according to published guidelines. The MTD for schedules A and B were 59 and 83 mg/m(2) , respectively. On schedule A, hepatotoxicity was dose limiting. Multi-organ failure (MOF) was dose limiting on schedule B. The overall complete (CR) and partial (PR) response rate in patients treated at or below the MTD was 17% (4/23, 2 CRs, 2 PRs) for schedule A and 10% (2/19, 2 PRs) for schedule B. Pevonedistat plasma concentrations peaked after infusion followed by elimination in a biphasic pattern. Pharmacodynamic studies of biological correlates of NAE inhibition demonstrated target-specific activity of pevonedistat. In conclusion, administration of the first-in-class agent, pevonedistat, was feasible in patients with MDS and AML and modest clinical activity was observed. © 2015 John Wiley & Sons Ltd.

  14. Effectiveness and student perceptions of an active learning activity using a headline news story to enhance in-class learning of cell cycle regulation.

    Science.gov (United States)

    Dirks-Naylor, Amie J

    2016-06-01

    An active learning activity was used to engage students and enhance in-class learning of cell cycle regulation in a PharmD level integrated biological sciences course. The aim of the present study was to determine the effectiveness and perception of the in-class activity. After completion of a lecture on the topic of cell cycle regulation, students completed a 10-question multiple-choice quiz before and after engaging in the activity. The activity involved reading of a headline news article published by ScienceDaily.com entitled "One Gene Lost Equals One limb Regained." The name of the gene was deleted from the article and, thus, the end goal of the activity was to determine the gene of interest by the description in the story. The activity included compiling a list of all potential gene candidates before sufficient information was given to identify the gene of interest (p21). A survey was completed to determine student perceptions of the activity. Quiz scores improved by an average of 20% after the activity (40.1 ± 1.95 vs. 59.9 ± 2.14,Pactivity, found the news article interesting, and believed that the activity improved their understanding of cell cycle regulation. The majority of students agreed that the in-class activity piqued their interest for learning the subject matter and also agreed that if they understand a concept during class, they are more likely to want to study that concept outside of class. In conclusion, the activity improved in-class understanding and enhanced interest in cell cycle regulation. Copyright © 2016 The American Physiological Society.

  15. A qualitative study of how to create supportive environments for the implementation of in-class-activities in middle school

    DEFF Research Database (Denmark)

    Holt, Anne-Didde; Smedegaard, Søren; Christiansen, Lars Breum Skov

    the intervention period, a case study comprising 10 semi-structured focus group interviews were carried out with 37 4th and 6th-grade students. Furthermore, the involved class teachers were interviewed. The interviews aimed to gain insight into students and teachers experiences with the implementation of ICA......Purpose School physical activity (SPA) holds the potential to benefit children’s psychosocial well-being. PA can positively influence mental health through social connectedness, physical self-perception, and improvements in emotions. For such reasons, in-class-activities (ICA) (e.g. energizers......, active breaks) has been given increased attention in the literature. However, if all students are to benefit from the potential mental health qualities of ICA, the right supportive environment is crucial. The purpose of this study was, on the basis of students and teachers feedback to an ICA...

  16. Novel Use of a Remote Laboratory for Active Learning in Class

    Science.gov (United States)

    Ramírez, Darinka; Ramírez, María Soledad; Marrero, Thomas R.

    2016-01-01

    This study aims to describe a novel teaching mode that allows for direct instructor-student and student-student discussions of material balance concepts by means of active learning. The instructor explains the concepts during class time while using a remotely controlled laboratory system that is projected on a screen with real-time access to the…

  17. The Influence of the Sport Education Model on Amotivated Students' In-Class Physical Activity

    Science.gov (United States)

    Perlman, Dana

    2012-01-01

    The Sport Education Model (SEM) was designed by Siedentop to provide students with a holistic sport-based experience. As research on the SEM continues, an aspect that has gained interest is the influence on (a) students with low levels of motivation and (b) opportunities to engage in health-enhancing levels of physical activity. The purpose of…

  18. Sertraline demonstrates fungicidal activity in vitro for Coccidioides immitis

    Directory of Open Access Journals (Sweden)

    Simon Paul

    2016-07-01

    Full Text Available Coccidioidomycosis causes substantial morbidity in endemic areas. Disseminated coccidioidomycosis is an AIDS defining condition and treatment often requires lifelong antifungal therapy. Sertraline, a widely used serotonin-reuptake inhibitor anti-depressant, has demonstrated activity against Candida and Cryptococcus sp. both in vitro and in vivo. To evaluate if sertraline has activity against Coccidioides, the minimal inhibitory concentration (MIC and minimal fungicidal concentration (MFC of sertraline for four clinical isolates of C. immitis were determined. Sertraline was observed to have an MIC range of 4–8 µg/ml and MFC also of 4–8 µg/ml for Coccidioides. These MIC and MFC results for C. immitis are similar to those reported for Cryptococcus sp. suggesting sertraline may potentially have utility for the treatment of coccidioidomycosis.

  19. Enhancing student retention of prerequisite knowledge through pre-class activities and in-class reinforcement.

    Science.gov (United States)

    Taylor, Ann T S; Olofson, Eric L; Novak, Walter R P

    2017-03-04

    To foster the connection between biochemistry and the supporting prerequisite concepts, a collection of activities that explicitly link general and organic chemistry concepts to biochemistry ideas was written and either assigned as pre-class work or as recitation activities. We assessed student learning gains after using these activities alone, or in combination with regularly-integrated clicker and discussion questions. Learning gains were determined from student performance on pre- and post-tests covering key prerequisite concepts, biochemistry course exams, and student self-evaluation. Long-term retention of the material was assessed using a comprehensive exam given to a subset of the students. Our results show that using the pre-class exercises in combination with integrative questions was effective at improving student performance in both the short and long term. Similar results were obtained at both a large research institution with large class enrollments and at a private liberal arts college with moderate enrollments. © 2016 by The International Union of Biochemistry and Molecular Biology, 45(2):97-104, 2017. © 2016 The International Union of Biochemistry and Molecular Biology.

  20. Last results of MADRAS, a space active optics demonstrator

    Science.gov (United States)

    Laslandes, Marie; Hourtoule, Claire; Hugot, Emmanuel; Ferrari, Marc; Devilliers, Christophe; Liotard, Arnaud; Lopez, Céline; Chazallet, Frédéric

    2017-11-01

    The goal of the MADRAS project (Mirror Active, Deformable and Regulated for Applications in Space) is to highlight the interest of Active Optics for the next generation of space telescope and instrumentation. Wave-front errors in future space telescopes will mainly come from thermal dilatation and zero gravity, inducing large lightweight primary mirrors deformation. To compensate for these effects, a 24 actuators, 100 mm diameter deformable mirror has been designed to be inserted in a pupil relay. Within the project, such a system has been optimized, integrated and experimentally characterized. The system is designed considering wave-front errors expected in 3m-class primary mirrors, and taking into account space constraints such as compactness, low weight, low power consumption and mechanical strength. Finite Element Analysis allowed an optimization of the system in order to reach a precision of correction better than 10 nm rms. A dedicated test-bed has been designed to fully characterize the integrated mirror performance in representative conditions. The test set up is made of three main parts: a telescope aberrations generator, a correction loop with the MADRAS mirror and a Shack-Hartman wave-front sensor, and PSF imaging. In addition, Fizeau interferometry monitors the optical surface shape. We have developed and characterized an active optics system with a limited number of actuators and a design fitting space requirements. All the conducted tests tend to demonstrate the efficiency of such a system for a real-time, in situ wave-front. It would allow a significant improvement for future space telescopes optical performance while relaxing the specifications on the others components.

  1. A Sport Education Fitness Season's Impact on Students' Fitness Levels, Knowledge, and In-Class Physical Activity.

    Science.gov (United States)

    Ward, Jeffery Kurt; Hastie, Peter A; Wadsworth, Danielle D; Foote, Shelby; Brock, Sheri J; Hollett, Nikki

    2017-09-01

    The purpose of this study was to determine the extent to which a sport education season of fitness could provide students with recommended levels of in-class moderate-to-vigorous physical activity (MVPA) while also increasing students' fitness knowledge and fitness achievement. One hundred and sixty-six 5th-grade students (76 boys, 90 girls) participated in a 20-lesson season called "CrossFit Challenge" during a 4-week period. The Progressive Aerobic Cardiovascular Endurance Run, push-ups, and curl-ups tests of the FITNESSGRAM® were used to assess fitness at pretest and posttest, while fitness knowledge was assessed through a validated, grade-appropriate test of health-related fitness knowledge (HRF). Physical activity was measured with Actigraph GT3X triaxial accelerometers. Results indicated a significant time effect for all fitness tests and the knowledge test. Across the entire season, the students spent an average of 54.5% of lesson time engaged in MVPA, irrespective of the type of lesson (instruction, free practice, or competition). The results suggest that configuring the key principles of sport education within a unit of fitness is an efficient model for providing students with the opportunity to improve fitness skill and HRF knowledge while attaining recommended levels of MVPA.

  2. Should Physical Activity Be Included in Nutrition Education? A Comparison of Nutrition Outcomes with and without In-Class Activities

    Science.gov (United States)

    Palmer-Keenan, Debra M.; Corda, Kirsten

    2014-01-01

    Limited-resource adults' dietary intakes and nutrition behaviors improve as a result of Expanded Food and Nutrition Education Program (EFNEP)/Supplemental Nutrition Assistance Program Education (SNAP-Ed) participation; however, physical activity education is needed for improved health. The experimental study reported here assessed if spending time…

  3. Air Pollution and Weather: Activities and Demonstrations for Science Classes

    Science.gov (United States)

    Cole, Henry S.

    1973-01-01

    Discusses a number of concepts (turbulence, dispersion, vertical temperature distribution, atmospheric stability and instability, and inversions) which are prerequisite to understanding how weather affects air quality. Describes classroom demonstrations effective in introducing these concepts to students at the elementary, secondary and college…

  4. Working Together in Class

    Directory of Open Access Journals (Sweden)

    Pateşan Marioara

    2017-12-01

    Full Text Available The scores obtained by the military students are very important as a lot of opportunities depend on them: the choice of the branch, selection for different in and off-campus activities, the appointment to the workplace and so on. A qualifier, regardless of its form of effective expression, can make a difference in a given context of issuing a value judgment, in relation to the student's performance assessment. In our research we tried to find out what motives students, what determines them to get actively involved in the tasks they are given and the ways we can improve their participation in classes and assignments. In order to have an educated generation we need to have not only well prepared teachers but ones that are open-minded, flexible and in pace with the methodological novelties that can improve the teaching learning process in class. Along the years we have noticed that in classes where students constituted a cohesive group with an increasing degree of interaction between members, the results were better than in a group that did not appreciate team-work. In this article we want to highlight the fact that a teacher can bring to class the appropriate methods and procedures can contribute decisively to the strengthening of the group cohesion and high scores.

  5. An Experiential Learning Activity Demonstrating Normal and Phobic Anxiety

    Science.gov (United States)

    Canu, Will H.

    2008-01-01

    This article describes an activity for an undergraduate abnormal psychology course that used student-generated data to illustrate normal versus clinically significant anxiety responses related to specific phobias. Students (N = 37) viewed 14 images of low- or high-anxiety valence and rated their subjective response to each. Discussion in a…

  6. Engaging Participation and Promoting Active Learning through Student Usage of the Internet to Create Notes for General Chemistry in Class

    Science.gov (United States)

    Henry, Renee Monica

    2017-01-01

    Reported here is a study of an interactive component to General Chemistry I and General Chemistry II where a new pedagogy for taking notes in class was developed. These notes, called key word created class notes, prompted students to locate information using the Internet guided by a key word. Reference Web sites were added to a next generation of…

  7. Demonstration of magnetically activated and guided isotope separation

    Science.gov (United States)

    Mazur, Thomas R.; Klappauf, Bruce; Raizen, Mark G.

    2014-08-01

    Enriched isotopes are widely used in medicine, basic science and energy production, and the need will only grow in the future. The main method for enriching stable isotopes today, the calutron, dates back over eighty years and has an uncertain future, creating an urgent need, especially in nuclear medicine. We report here the experimental realization of a general and efficient method for isotope separation that presents a viable alternative to the calutron. Combining optical pumping and a unique magnet geometry, we observe substantial depletion of Li-6 throughput in a lithium atomic beam produced by an evaporation source over a range of flux. These results demonstrate the viability of our method to yield large degrees of enrichment in a manner that is amenable to industrial scale-up and the production of commercially relevant quantities.

  8. Review of Experimental Compounds Demonstrating Anti-Toxoplasma Activity

    Science.gov (United States)

    McFarland, Madalyn M.; Zach, Sydney J.; Wang, Xiaofang; Potluri, Lakshmi-Prasad; Neville, Andrew J.; Vennerstrom, Jonathan L.

    2016-01-01

    Toxoplasma gondii is a ubiquitous apicomplexan parasite capable of infecting humans and other animals. Current treatment options for T. gondii infection are limited and most have drawbacks, including high toxicity and low tolerability. Additionally, no FDA-approved treatments are available for pregnant women, a high-risk population due to transplacental infection. Therefore, the development of novel treatment options is needed. To aid this effort, this review highlights experimental compounds that, at a minimum, demonstrate inhibition of in vitro growth of T. gondii. When available, host cell toxicity and in vivo data are also discussed. The purpose of this review is to facilitate additional development of anti-Toxoplasma compounds and potentially to extend our knowledge of the parasite. PMID:27600037

  9. Four minutes of in-class high-intensity interval activity improves selective attention in 9- to 11-year olds.

    Science.gov (United States)

    Ma, Jasmin K; Le Mare, Lucy; Gurd, Brendon J

    2015-03-01

    The amount of time allocated to physical activity in schools is declining. Time-efficient physical activity solutions that demonstrate their impact on academic achievement-related outcomes are needed to prioritize physical activity within the school curricula. "FUNtervals" are 4-min, high-intensity interval activities that use whole-body actions to complement a storyline. The purpose of this study was to (i) explore whether FUNtervals can improve selective attention, an executive function posited to be essential for learning and academic success; and (ii) examine whether this relationship is predicted by students' classroom off-task behaviour. Seven grade 3-5 classes (n = 88) were exposed to a single-group, repeated cross-over design where each student's selective attention was compared between no-activity and FUNtervals days. In week 1, students were familiarized with the d2 test of attention and FUNterval activities, and baseline off-task behaviour was observed. In both weeks 2 and 3 students completed the d2 test of attention following either a FUNterval break or a no-activity break. The order of these breaks was randomized and counterbalanced between weeks. Neither motor nor passive off-task behaviour predicted changes in selective attention following FUNtervals; however, a weak relationship was observed for verbal off-task behaviour and improvements in d2 test performance. More importantly, students made fewer errors during the d2 test following FUNtervals. In supporting the priority of physical activity inclusion within schools, FUNtervals, a time efficient and easily implemented physical activity break, can improve selective attention in 9- to 11-year olds.

  10. Civility in Classes and Sports

    Science.gov (United States)

    Lumpkin, Angela

    2010-01-01

    Civility is a polite or courteous act, expression, or standard of conduct, including the display of respect and tolerance to everyone. Teaching and modeling civility in classes and with sport teams is essential so students and athletes can learn the importance of and demonstrate civility in their interactions with others. Teachers and coaches…

  11. Superantigen and HLA-DR ligation induce phospholipase-C gamma 1 activation in class II+ T cells

    DEFF Research Database (Denmark)

    Kanner, S B; Odum, Niels; Grosmaire, L

    1992-01-01

    activated by HLA-DR ligation through antibody cross-linking or by direct enterotoxin superantigen binding. Both types of stimuli induced tyrosine phosphorylation of phosphatidylinositol-specific phospholipase C gamma 1 (PLC gamma 1) and an increase in intracellular calcium concentration; however......, superantigen-induced signaling was stronger than class II ligation alone. Antibody-mediated ligation of HLA-DR with CD3 resulted in augmented PLC gamma 1 activation and increased calcium mobilization, consistent with a mechanism of superantigen activity through a combination of class II and CD3/Ti signals...... engagement and PMA addition. In addition, superantigen-induced growth was induced by CD28 receptor ligation with antibody or the B7 counter-receptor expressed on Chinese hamster ovary cells. Taken together, these results indicate that class II molecules expressed on activated T cells are directly coupled...

  12. Coming Out in Class: Challenges and Benefits of Active Learning in a Biology Classroom for LGBTQIA Students

    Science.gov (United States)

    Cooper, Katelyn M.; Brownell, Sara E.

    2016-01-01

    As we transition our undergraduate biology classrooms from traditional lectures to active learning, the dynamics among students become more important. These dynamics can be influenced by student social identities. One social identity that has been unexamined in the context of undergraduate biology is the spectrum of lesbian, gay, bisexual, transgender, queer, intersex, and asexual (LGBTQIA) identities. In this exploratory interview study, we probed the experiences and perceptions of seven students who identify as part of the LGBTQIA community. We found that students do not always experience the undergraduate biology classroom to be a welcoming or accepting place for their identities. In contrast to traditional lectures, active-learning classes increase the relevance of their LGBTQIA identities due to the increased interactions among students during group work. Finally, working with other students in active-learning classrooms can present challenges and opportunities for students considering their LGBTQIA identity. These findings indicate that these students’ LGBTQIA identities are affecting their experience in the classroom and that there may be specific instructional practices that can mitigate some of the possible obstacles. We hope that this work can stimulate discussions about how to broadly make our active-learning biology classes more inclusive of this specific population of students. PMID:27543636

  13. Coming out in Class: Challenges and Benefits of Active Learning in a Biology Classroom for LGBTQIA Students

    Science.gov (United States)

    Cooper, Katelyn M.; Brownell, Sara E.

    2016-01-01

    As we transition our undergraduate biology classrooms from traditional lectures to active learning, the dynamics among students become more important. These dynamics can be influenced by student social identities. One social identity that has been unexamined in the context of undergraduate biology is the spectrum of lesbian, gay, bisexual,…

  14. Coming Out in Class: Challenges and Benefits of Active Learning in a Biology Classroom for LGBTQIA Students.

    Science.gov (United States)

    Cooper, Katelyn M; Brownell, Sara E

    As we transition our undergraduate biology classrooms from traditional lectures to active learning, the dynamics among students become more important. These dynamics can be influenced by student social identities. One social identity that has been unexamined in the context of undergraduate biology is the spectrum of lesbian, gay, bisexual, transgender, queer, intersex, and asexual (LGBTQIA) identities. In this exploratory interview study, we probed the experiences and perceptions of seven students who identify as part of the LGBTQIA community. We found that students do not always experience the undergraduate biology classroom to be a welcoming or accepting place for their identities. In contrast to traditional lectures, active-learning classes increase the relevance of their LGBTQIA identities due to the increased interactions among students during group work. Finally, working with other students in active-learning classrooms can present challenges and opportunities for students considering their LGBTQIA identity. These findings indicate that these students' LGBTQIA identities are affecting their experience in the classroom and that there may be specific instructional practices that can mitigate some of the possible obstacles. We hope that this work can stimulate discussions about how to broadly make our active-learning biology classes more inclusive of this specific population of students. © 2016 K. M. Cooper and S. E. Brownell. CBE—Life Sciences Education © 2016 The American Society for Cell Biology. This article is distributed by The American Society for Cell Biology under license from the author(s). It is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

  15. Pevonedistat, a first-in-class NEDD8-activating enzyme (NAE) inhibitor, combined with azacitidine, in patients with AML.

    Science.gov (United States)

    Swords, Ronan T; Coutre, Steven; Maris, Michael B; Zeidner, Joshua F; Foran, James M; Cruz, Jose; Erba, Harry P; Berdeja, Jesus G; Tam, Wayne; Vardhanabhuti, Saran; Pawlikowska-Dobler, Iwona; Faessel, Hélène M; Dash, Ajeeta B; Sedarati, Farhad; Dezube, Bruce J; Faller, Douglas V; Savona, Michael R

    2018-01-18

    Pevonedistat (TAK-924/MLN4924) is a novel inhibitor of NEDD8-activating enzyme (NAE) with single-agent activity in relapsed/refractory acute myeloid leukemia (AML). We performed a phase 1b study (NCT01814826) of pevonedistat (PEV) with azacitidine (AZA) based on synergistic activity seen preclinically. Primary objectives included safety and tolerability, and secondary objectives included pharmacokinetics (PK) and disease response. Patients ≥60 years with treatment-naïve AML, unfit for standard induction therapy, received PEV 20 or 30 mg/m 2 IV on days 1, 3, and 5, combined with fixed-dose AZA (75 mg/m 2 IV/SC) on days 1-5, 8, and 9, every 28 days. The most common treatment-emergent adverse events were constipation (48%), nausea (42%), fatigue (42%), and anemia (39%). In total, 11 deaths were observed and considered unrelated to study therapy by the investigators. Transient elevations in AST and ALT were dose limiting. The recommended phase 2 dose of PEV in this combination is 20 mg/m 2 PEV PK was not altered by the addition of AZA. Overall response rate (ORR) based on an ITT analysis was 50% (20 CR, 5 CRi, 7 PR), with an 8.3-month median duration of remission. In patients receiving ≥6 cycles of therapy (n = 23, 44%), ORR was 83%. In patients with TP53 mutations, the composite CR/PR rate was 80% (4/5). Two of these patients stayed on study for >10 cycles. Baseline bone marrow blast percentage or cytogenetic/molecular risk did not influence ORR. Copyright © 2018 American Society of Hematology.

  16. Miller Creek Demonstration Forest ecology activities - a teachers supplement to the field guide

    Science.gov (United States)

    Bill Schustrom; Reed Kuennen; Raymond C. Shearer

    1998-01-01

    Miller Creek, on the Flathead National Forest in northwestern Montana, is a demonstration forest, showing up to 30 years of forest change. This teachers supplement to the educational field guide (Miller Creek Demonstration Forest - a forest born of fire: a field guide; Gen. Tech. Rep. RMRS-GTR-7, 1998) outlines eight field and classroom activities that teach students a...

  17. First-Year and Non-First-Year Student Expectations Regarding In-Class and Out-of-Class Learning Activities in Introductory Biology

    Directory of Open Access Journals (Sweden)

    Tanya Louise Brown

    2017-05-01

    Full Text Available National calls for teaching transformation build on a constructivist learning theory and propose that students learn by actively engaging in course activities and interacting with other students. While interactive pedagogies can improve learning, they also have the potential to challenge traditional norms regarding class participation and learning strategies. To better understand the potential openness of students to interactive teaching practices, we administered a survey during the first week of two sections of an introductory biology course to characterize how students envisioned spending time during class as well as what activities they expected to complete outside of class during non-exam weeks and in preparation for exams. Additionally, we sought to test the hypothesis that the expectations of first-year students differed from those of non-first-year students. Analyses of closed-ended and open-ended questions revealed that students held a wide range of expectations and that most students expressed expectations consistent with some degree of transformed teaching. Furthermore, first-year students expected more active learning in class, more out-ofclass coursework during non-exam weeks, and more social learning strategies than non-first-year students. We discuss how instructor awareness of incoming student expectations might be used to promote success in introductory science courses.

  18. First-Year and Non-First-Year Student Expectations Regarding In-Class and Out-of-Class Learning Activities in Introductory Biology.

    Science.gov (United States)

    Brown, Tanya L; Brazeal, Kathleen R; Couch, Brian A

    2017-04-01

    National calls for teaching transformation build on a constructivist learning theory and propose that students learn by actively engaging in course activities and interacting with other students. While interactive pedagogies can improve learning, they also have the potential to challenge traditional norms regarding class participation and learning strategies. To better understand the potential openness of students to interactive teaching practices, we administered a survey during the first week of two sections of an introductory biology course to characterize how students envisioned spending time during class as well as what activities they expected to complete outside of class during non-exam weeks and in preparation for exams. Additionally, we sought to test the hypothesis that the expectations of first-year students differed from those of non-first-year students. Analyses of closed-ended and open-ended questions revealed that students held a wide range of expectations and that most students expressed expectations consistent with some degree of transformed teaching. Furthermore, first-year students expected more active learning in class, more out-of-class coursework during non-exam weeks, and more social learning strategies than non-first-year students. We discuss how instructor awareness of incoming student expectations might be used to promote success in introductory science courses.

  19. Effects of cadmium exposure on glycogen phosphorylase activity in rat placenta as demonstrated by histochemical means

    NARCIS (Netherlands)

    Hazelhoff Roelfzema, W.; Hacker, H. J.; van Noorden, C. J.

    1989-01-01

    Glycogen phosphorylase (PHO) activity was demonstrated histochemically in unfixed cryostat sections of placentae from cadmium-treated and control rats with the use of the semipermeable membrane technique. Staining of the newly synthesized glycogen was performed by lugol. A high activity was present

  20. Demonstrations of the Activity Theory Framework for Research in Information Systems

    Directory of Open Access Journals (Sweden)

    Kate Crawford

    2006-05-01

    Full Text Available The holistic and insightful nature of the (cultural-historical Activity Theory has led to its use by several researchers as a suitable vehicle for understanding and analysis in many areas of IS research and practice. This paper demonstrates the application of Activity Theory to the study of socio-technical systems which mediate complex, collective activities in the modern workplace and in everyday life. Vignettes from five ongoing research projects are reported in order to illustrate not only the explanatory power of the Activity Theory research framework but also its use in determining appropriate methods used to manage the data collection and analysis processes as well as its interpretation., The paper demonstrates the variety of IS topics where an Activity Theory based approach is able to add richness and insight.

  1. Authentic active learning activities demonstrating the use of serial dilutions and plate counts.

    Science.gov (United States)

    March, Jordon K; Jensen, Kyle C; Porter, Nathan T; Breakwell, Donald P

    2011-01-01

    Serial dilution and plate counting is often taught in courses for both microbiology and allied health students. Lecture examples and examination questions addressing how the method is used can sometimes be contrived: artificial data sets may have little or no meaning other than to have students perform a calculation. Here we provide a set of activities employing data sets acquired from the primary literature. Our objective was to have the students think critically about a real scenario in which serial dilution and plate count was used. Each activity requires students to read a paragraph describing the study, predict the results, perform the appropriate calculations, and then evaluate the results in light of their predictions. To test the efficacy of these activities, a pretest quiz was given to approximately 100 students in an allied health/general microbiology course. After a lecture on how microbes are enumerated, students were given a different quiz. The class was then divided randomly into groups of three or four students and assigned one of the activities. A postactivity quiz was also administered. Approximately two weeks later, a serial dilution/plate count question was used on an examination and served as a final posttest. Standardized learning gains were calculated for the quiz administered after each learning activity. Even though learning gains were significantly higher after the lecture, there was also a significant improvement between the lecture and the activity. Using an exercise based on an authentic set of data significantly improved student learning gains, and is a useful practice for teaching microbiology.

  2. Integrated gasification combined-cycle research development and demonstration activities in the US

    Energy Technology Data Exchange (ETDEWEB)

    Ness, H.M.; Brdar, R.D.

    1996-09-01

    The United States Department of Energy (DOE)`s Office of Fossil Energy, Morgantown Energy Technology Center, is managing a research development and demonstration (RD&D) program that supports the commercialization of integrated gasification combined-cycle (IGCC) advanced power systems. This overview briefly describes the supporting RD&D activities and the IGCC projects selected for demonstration in the Clean Coal Technology (CCT) Program.

  3. Early Site Permit Demonstration Program: Recommendations for communication activities and public participation in the Early Site Permit Demonstration Program

    International Nuclear Information System (INIS)

    1993-01-01

    On October 24, 1992, President Bush signed into law the National Energy Policy Act of 1992. The bill is a sweeping, comprehensive overhaul of the Nation's energy laws, the first in more than a decade. Among other provisions, the National Energy Policy Act reforms the licensing process for new nuclear power plants by adopting a new approach developed by the US Nuclear Regulatory Commission (NRC) in 1989, and upheld in court in 1992. The NRC 10 CFR Part 52 rule is a three-step process that guarantees public participation at each step. The steps are: early site permit approval; standard design certifications; and, combined construction/operating licenses for nuclear power reactors. Licensing reform increases an organization's ability to respond to future baseload electricity generation needs with less financial risk for ratepayers and the organization. Costly delays can be avoided because design, safety and siting issues will be resolved before a company starts to build a plant. Specifically, early site permit approval allows for site suitability and acceptability issues to be addressed prior to an organization's commitment to build a plant. Responsibility for site-specific activities, including communications and public participation, rests with those organizations selected to try out early site approval. This plan has been prepared to assist those companies (referred to as sponsoring organizations) in planning their communications and public involvement programs. It provides research findings, information and recommendations to be used by organizations as a resource and starting point in developing their own plans

  4. Early Site Permit Demonstration Program: Recommendations for communication activities and public participation in the Early Site Permit Demonstration Program

    Energy Technology Data Exchange (ETDEWEB)

    1993-01-27

    On October 24, 1992, President Bush signed into law the National Energy Policy Act of 1992. The bill is a sweeping, comprehensive overhaul of the Nation`s energy laws, the first in more than a decade. Among other provisions, the National Energy Policy Act reforms the licensing process for new nuclear power plants by adopting a new approach developed by the US Nuclear Regulatory Commission (NRC) in 1989, and upheld in court in 1992. The NRC 10 CFR Part 52 rule is a three-step process that guarantees public participation at each step. The steps are: early site permit approval; standard design certifications; and, combined construction/operating licenses for nuclear power reactors. Licensing reform increases an organization`s ability to respond to future baseload electricity generation needs with less financial risk for ratepayers and the organization. Costly delays can be avoided because design, safety and siting issues will be resolved before a company starts to build a plant. Specifically, early site permit approval allows for site suitability and acceptability issues to be addressed prior to an organization`s commitment to build a plant. Responsibility for site-specific activities, including communications and public participation, rests with those organizations selected to try out early site approval. This plan has been prepared to assist those companies (referred to as sponsoring organizations) in planning their communications and public involvement programs. It provides research findings, information and recommendations to be used by organizations as a resource and starting point in developing their own plans.

  5. Data analysis on work activities in dismantling of Japan Power Demonstration Reactor (JPDR). Contract research

    International Nuclear Information System (INIS)

    Shiraishi, Kunio; Sukegawa, Takenori; Yanagihara, Satoshi

    1998-03-01

    The safe dismantling of a retired nuclear power plant was demonstrated by completion of dismantling activities for the Japan Power Demonstration Reactor (JPDR), March, 1996, which had been conducted since 1986. This project was a flag ship project for dismantling of nuclear power plants in Japan, aiming at demonstrating an applicability of developed dismantling techniques in actual dismantling work, developing database on work activities as well as dismantling of components and structures. Various data on dismantling activities were therefore systematically collected and these were accumulated on computer files to build the decommissioning database; dismantling activities were characterized by analyzing the data. The data analysis resulted in producing general forms such as unit activity factors, for example, manpower need per unit weight of component to be dismantled, and simple arithmetic forms for forecasting of project management data to be applied to planning another dismantling project through the evaluation for general use of the analyzed data. The results of data analysis could be usefully applied to planning of future decommissioning of commercial nuclear power plants in Japan. This report describes the data collection and analysis on the JPDR dismantling activities. (author)

  6. An Anesthetic Drug Demonstration and an Introductory Antioxidant Activity Experiment with "Eugene, the Sleepy Fish"

    Science.gov (United States)

    Barcena, Homar; Chen, Peishan

    2016-01-01

    Students are introduced to spectrophotometry in comparing the antioxidant activity of pure eugenol and oil of cloves from a commercial source using a modified ferric reducing antioxidant power (FRAP) assay. The extraction of the essential oil from dried cloves is demonstrated to facilitate discussions on green chemistry. The anesthetic properties…

  7. Locating REDD: A global survey and analysis of REDD readiness and demonstration activities

    International Nuclear Information System (INIS)

    Cerbu, Gillian A.; Swallow, Brent M.; Thompson, Dara Y.

    2011-01-01

    Mechanisms that support reduced emissions from deforestation and forest degradation (REDD/REDD+) have potential to counteract a large share of global greenhouse gas emissions if implemented effectively across the tropics. In 2007 the conference of parties to the United Nations Framework Convention on Climate Change called upon parties and international organizations to promote REDD through investments in capacity building and demonstration activities. This prompted many new actors to become involved in REDD activities at a variety of locations and scales. A global survey of REDD activities was undertaken in 2009 to enable better understanding of the intensity and geographic distribution of these activities. Existing compilations, literature review, web-based sources, face-to-face and telephone interviews, and e-mail questionnaires were used to compile data for the inventory. Inter alia, data were collected on the location of activities and official and unofficial factors influencing location choices. Inventory data were combined with secondary data to estimate a statistical count model (Poisson) of factors affecting the number of REDD activities undertaken in the 64 developing countries that experienced significant emissions from deforestation. The results show that there were at least 79 REDD readiness activities and 100 REDD demonstration activities as of October 2009. Of these, the largest shares of REDD readiness and demonstration activities were implemented in Indonesia (7 and 15 respectively) and Brazil (4 and 13 respectively), countries widely agreed to have the greatest potential for reducing forest-based emissions. The statistical results found no national characteristic to have a statistically-significant effect on the number of REDD readiness activities, but five national characteristics to have significant effects on the number of REDD demonstration projects. Baseline CO 2 emissions, forest carbon stock, number of threatened species, quality of

  8. [Ecological demonstration activity and eco-civilization construction mode: review and prospects].

    Science.gov (United States)

    Mao, Hui-ping; He, Xuan; He, Jia; Niu, Dong-jie; Bao, Cun-kuan

    2013-04-01

    Ecological civilization is to normalize human development behaviors to harmonize the relationships between social and ecological development and eco-environment protection. In this paper, a comparative analysis was made on the ecological demonstration activities of ecological demonstration areas led by the Ministry of Environmental Protection, exemplar cities of national environmental protection, and ecological provinces, cities, and counties. It was considered that all the ecological demonstration activities had the problems of lacking pertinence of construction goals, disordered construction subjects, inefficient construction processes, and lacking continuous incentive mechanisms of assessment. In the meantime, through the analysis of the connotations of eco-civilization, the relationships between eco-civilization and eco-demonstration constructions were approached, and the eco-civilization construction mode was put forward in terms of construction goal, construction subject, and construction processes and assessment. The construction mode included the construction goal based on regional characteristics; the synergistic cooperation of construction subjects, the expanding ways of public participation, and the establishment of evaluation system for comprehensively measuring the 'actions and results'.

  9. PA activity by using nuclear power plant safety demonstration and analysis

    International Nuclear Information System (INIS)

    Tsuchiya, Mitsuo; Kamimae, Rie

    1999-01-01

    INS/NUPEC presents one of Public acceptance (PA) methods for nuclear power in Japan, 'PA activity by using Nuclear Power Plant Safety Demonstration and Analysis', by using one of videos which is explained and analyzed accident events (Loss of Coolant Accident). Safety regulations of The National Government are strictly implemented in licensing at each of basic design and detailed design. To support safety regulation activities conducted by the National Government, INS/NLTPEC continuously implement Safety demonstration and analysis. With safety demonstration and analysis, made by assuming some abnormal conditions, what impacts could be produced by the assumed conditions are forecast based on specific design data on a given nuclear power plants. When analysis results compared with relevant decision criteria, the safety of nuclear power plants is confirmed. The decision criteria are designed to help judge if or not safety design of nuclear power plants is properly made. The decision criteria are set in the safety examination guidelines by taking sufficient safety allowance based on the latest technical knowledge obtained from a wide range of tests and safety studies. Safety demonstration and analysis is made by taking the procedure which are summarized in this presentation. In Japan, various PA (Public Acceptance) pamphlets and videos on nuclear energy have been published. But many of them focused on such topics as necessity or importance of nuclear energy, basic principles of nuclear power generation, etc., and a few described safety evaluation particularly of abnormal and accident events in accordance with the regulatory requirements. In this background, INS/NUPEC has been making efforts to prepare PA pamphlets and videos to explain the safety of nuclear power plants, to be simple and concrete enough, using various analytical computations for abnormal and accident events. In results, PA activity of INS/NUPEC is evaluated highly by the people

  10. Demonstration in human plasma of a lectin activity analogous to that of bovine conglutinin

    DEFF Research Database (Denmark)

    Baatrup, G; Thiel, S; Isager, H

    1987-01-01

    Evidence of the existence in human plasma of an activity analogous to that of bovine conglutinin is presented. The human plasma component was characterized antigenically and functionally. Human plasma was shown to agglutinate complement-coated erythrocytes in the presence of Ca2+, and this conglu...... in plasma. The activity was more stable in plasma containing metal-ion chelators like EDTA and citrate than in heparin or hirudin plasma....... raised against bovine conglutinin cross-react with the human protein. The plasma concentration of the conglutinin-like protein showed a high inter-individual variation between apparently healthy donors. Unlike bovine conglutinin, the human conglutinin activity could not be demonstrated in serum but only...

  11. Demonstration of prosthetic activation of central auditory pathways using [14C]-2-deoxyglucose

    International Nuclear Information System (INIS)

    Evans, D.A.; Niparko, J.K.; Altschuler, R.A.; Frey, K.A.; Miller, J.M.

    1990-01-01

    The cochlear prosthesis is not applicable to patients who lack an implantable cochlea or an intact vestibulocochlear nerve. Direct electrical stimulation of the cochlear nucleus (CN) of the brain stem might provide a method for auditory rehabilitation of these patients. A penetrating CN electrode has been developed and tissue tolerance to this device demonstrated. This study was undertaken to evaluate metabolic activation of central nervous system (CNS) auditory tracts produced by such implants. Regional cerebral glucose use resulting from CN stimulation was estimated in a series of chronically implanted guinea pigs with the use of [ 14 C]-2-deoxyglucose (2-DG). Enhanced 2-DG uptake was observed in structures of the auditory tract. The activation of central auditory structures achieved with CN stimulation was similar to that produced by acoustic stimulation and by electrical stimulation of the modiolar portion of the auditory nerve in control groups. An interesting banding pattern was observed in the inferior colliculus following CN stimulation, as previously described with acoustic stimulation. This study demonstrates that functional metabolic activation of central auditory pathways can be achieved with a penetrating CNS auditory prosthesis

  12. Demonstration of prosthetic activation of central auditory pathways using ( sup 14 C)-2-deoxyglucose

    Energy Technology Data Exchange (ETDEWEB)

    Evans, D.A.; Niparko, J.K.; Altschuler, R.A.; Frey, K.A.; Miller, J.M. (Univ. of Michigan Medical Center, Ann Arbor (USA))

    1990-02-01

    The cochlear prosthesis is not applicable to patients who lack an implantable cochlea or an intact vestibulocochlear nerve. Direct electrical stimulation of the cochlear nucleus (CN) of the brain stem might provide a method for auditory rehabilitation of these patients. A penetrating CN electrode has been developed and tissue tolerance to this device demonstrated. This study was undertaken to evaluate metabolic activation of central nervous system (CNS) auditory tracts produced by such implants. Regional cerebral glucose use resulting from CN stimulation was estimated in a series of chronically implanted guinea pigs with the use of ({sup 14}C)-2-deoxyglucose (2-DG). Enhanced 2-DG uptake was observed in structures of the auditory tract. The activation of central auditory structures achieved with CN stimulation was similar to that produced by acoustic stimulation and by electrical stimulation of the modiolar portion of the auditory nerve in control groups. An interesting banding pattern was observed in the inferior colliculus following CN stimulation, as previously described with acoustic stimulation. This study demonstrates that functional metabolic activation of central auditory pathways can be achieved with a penetrating CNS auditory prosthesis.

  13. Radioactive Demonstrations Of Fluidized Bed Steam Reforming (FBSR) With Hanford Low Activity Wastes

    Energy Technology Data Exchange (ETDEWEB)

    Jantzen, C. M.; Crawford, C. L.; Burket, P. R.; Bannochie, C. J.; Daniel, W. G.; Nash, C. A.; Cozzi, A. D.; Herman, C. C.

    2012-10-22

    Several supplemental technologies for treating and immobilizing Hanford low activity waste (LAW) are being evaluated. One immobilization technology being considered is Fluidized Bed Steam Reforming (FBSR) which offers a low temperature (700-750?C) continuous method by which wastes high in organics, nitrates, sulfates/sulfides, or other aqueous components may be processed into a crystalline ceramic (mineral) waste form. The granular waste form produced by co-processing the waste with kaolin clay has been shown to be as durable as LAW glass. The FBSR granular product will be monolithed into a final waste form. The granular component is composed of insoluble sodium aluminosilicate (NAS) feldspathoid minerals such as sodalite. Production of the FBSR mineral product has been demonstrated both at the industrial, engineering, pilot, and laboratory scales on simulants. Radioactive testing at SRNL commenced in late 2010 to demonstrate the technology on radioactive LAW streams which is the focus of this study.

  14. Demonstration of physical phenomenas and scavenging activity from d-psicose and methionine maillard reaction products

    Directory of Open Access Journals (Sweden)

    Arum Tiyas Suminar

    2017-01-01

    Full Text Available Maillard reaction has been well understood as a non-enzymatic reaction between reducing sugars and amino acids to generate the Maillard reaction products (MRPs. This study is aimed to demonstrate the browning intensity, color development, spectra measurements, scavenging activity, and the correlation between browning intensity and scavenging activity of the MRPs generated from D-Psicose and Methionine (Psi-Met at 50℃. The browning intensity of MRPs was investigated based on the absorbance using spectrophotometer at 420 nm, the color development was observed using digital colorimeter to gained L*a*b* value then calculated as browning index, the spectra development was analyzed using spectrophotometer at 190 - 750 nm, and the scavenging activity was determined with ABTS method using spectrophotometer at 734 nm. The browning intensity, color development, and scavenging activity were improved along with the increase in heating process. Based on spectra analysis, MRPs from Psi-Met was initially detected at 21 h and Psi at 24 h of heating treatment, which indicating that Psi-Met have faster and better reaction than Psi during heating process. Positive non-linear and significant correlation between browning intensity and scavenging activity were assigned. This finding may provide beneficial information of D-psicose and MRPs to the next scientific research and to the food industries which applies MRPs in their products.

  15. The impact of initiation: Early onset marijuana smokers demonstrate altered Stroop performance and brain activation

    Directory of Open Access Journals (Sweden)

    K.A. Sagar

    2015-12-01

    Full Text Available Marijuana (MJ use is on the rise, particularly among teens and emerging adults. This poses serious public health concern, given the potential deleterious effects of MJ on the developing brain. We examined 50 chronic MJ smokers divided into early onset (regular MJ use prior to age 16; n = 24 and late onset (age 16 or later; n = 26, and 34 healthy control participants (HCs. All completed a modified Stroop Color Word Test during fMRI. Results demonstrated that MJ smokers exhibited significantly poorer performance on the Interference subtest of the Stroop, as well as altered patterns of activation in the cingulate cortex relative to HCs. Further, early onset MJ smokers exhibited significantly poorer performance relative to both HCs and late onset smokers. Additionally, earlier age of MJ onset as well as increased frequency and magnitude (grams/week of MJ use were predictive of poorer Stroop performance. fMRI results revealed that while late onset smokers demonstrated a more similar pattern of activation to the control group, a different pattern was evident in the early onset group. These findings underscore the importance of assessing age of onset and patterns of MJ use and support the need for widespread education and intervention efforts among youth.

  16. Purification of highly active alphavirus replication complexes demonstrates altered fractionation of multiple cellular membranes.

    Science.gov (United States)

    Pietilä, Maija K; van Hemert, Martijn J; Ahola, Tero

    2018-01-24

    Positive-strand RNA viruses replicate their genomes in membrane-associated structures; alphaviruses and many other groups induce membrane invaginations called spherules. Here, we established a protocol to purify these membranous replication complexes (RCs) from cells infected with Semliki Forest virus (SFV). We isolated SFV spherules located on the plasma membrane and further purified them using two consecutive density gradients. This revealed that SFV infection strongly modifies cellular membranes. We removed soluble proteins, the Golgi and most of the mitochondria, but plasma membrane, endoplasmic reticulum (ER) and late endosome markers enriched in the membrane fraction that contained viral RNA synthesizing activity, replicase proteins and minus- and plus-strand RNA. Electron microscopy revealed that the purified membranes displayed spherule-like structures with a narrow neck. This membrane enrichment was specific to viral replication as such a distribution of membrane markers was only observed after infection. Besides the plasma membrane, SFV infection remodeled the ER, and the co-fractionation of the RC-carrying plasma membrane and ER suggests that SFV may recruit ER proteins or membrane to the site of replication. The purified RCs were highly active in synthesizing both genomic and subgenomic RNA. Detergent solubilization destroyed the replication activity demonstrating that the membrane association of the complex is essential. Most of the newly made RNA was in double-stranded replicative molecules but the purified complexes also produced single-stranded RNA as well as released newly made RNA. This indicates that the purification established here maintained the functionality of RCs and thus enables further structural and functional studies of active RCs. IMPORTANCE Similar to all positive-strand RNA viruses, the arthropod-borne alphaviruses induce membranous genome factories but little is known about the arrangement of viral replicase proteins and the

  17. Direct conscious telemetry recordings demonstrate increased renal sympathetic nerve activity in rats with chronic kidney disease

    Directory of Open Access Journals (Sweden)

    Ibrahim M Salman

    2015-08-01

    Full Text Available Chronic kidney disease (CKD is associated with sympathetic hyperactivity and impaired blood pressure control reflex responses, yet direct evidence demonstrating these features of autonomic dysfunction in conscious animals is still lacking. Here we measured renal sympathetic nerve activity (RSNA and mean arterial pressure (MAP using telemetry-based recordings in a rat model of CKD, the Lewis Polycystic Kidney (LPK rat, and assessed responses to chemoreflex activation and acute stress. Male LPK and Lewis control animals (total n=16 were instrumented for telemetric recording of RSNA and MAP. At 12–13 weeks-of-age, resting RSNA and MAP, sympathetic and haemodynamic responses to both peripheral (hypoxia: 10% O2 and central chemoreflex (hypercapnia: 7% CO2 activation and acute stress (open-field exposure, were measured. As indicators of renal function, urinary protein (UPro and creatinine (Ucr levels were assessed. LPK rats had higher resting RSNA (1.2±0.1 vs. 0.6±0.1 µV, p<0.05 and MAP (151±8 vs. 97±2 mmHg, p<0.05 compared to Lewis. MAP was negatively correlated with Ucr (r=-0.80, p=0.002 and positively correlated with RSNA (r=0.66, p=0.014, with multiple linear regression modeling indicating the strongest correlation was with Ucr. RSNA and MAP responses to activation of the central chemoreflex and open-field stress were reduced in the LPK relative to the Lewis (all p<0.05. This is the first description of dual conscious telemetry recording of RSNA and MAP in a genetic rodent model of CKD. Elevated RSNA is likely a key contributor to the marked hypertension in this model, while attenuated RSNA and MAP responses to central chemoreflex activation and acute stress in the LPK indicate possible deficits in the neural processing of autonomic outflows evoked by these sympathoexcitatory pathways.

  18. Tetrahydroxystilbene glucoside isolated from Polygonum multiflorum Thunb. demonstrates osteoblast differentiation promoting activity

    Science.gov (United States)

    Zheng, Yayuan; Li, Jin; Wu, Jingkai; Yu, Yongjie; Yao, Weimin; Zhou, Manru; Tian, Jun; Zhang, Jingjing; Cui, Liao; Zeng, Xiaobin; Liu, Yuyu

    2017-01-01

    Polygonum multiflorum Thunb. is a traditional Chinese medicinal herb that has been widely used to treat age-associated diseases. Tetrahydroxystilbene glucoside (TSG), also known as 2,3,5,4-tetrahydroxystilbene-2-O-β-D-glucoside, is a major component of this herb. The present study was designed to investigate the osteogenic differentiation promoting activity of TSG in rat mesenchymal stem cells (MSCs) and in zebrafish. Preliminary experiments using MTT assay and ALP methods indicate that the high potential activity for promoting osteogenic differentiation was observed when 50% ethanol eluate was used. Further isolation and purification of TSG from the 50% ethanol eluate was performed by bioassay-guided fractionation, and its structure was confirmed using nuclear magnetic resonance and mass spectrometry analyses. In addition, the relative content of TSG with the highest potential activity in the promotion of osteogenic differentiation was identified as 14.34% by reversed-phase high performance liquid chromatography. Subsequently, the osteogenic differentiation promoting abilities of TSG in MSCs were examined. The results demonstrated that TSG promoted the alkaline phosphatase activity at concentrations of 1.56–25 µg/ml, while it increased the content of osteocalcin 7 days after treatment with 6.25–25 µg/ml in MSCs. Furthermore, experiments in zebrafish indicated that different concentrations of TSG (3.12–12.5 µg/ml) protected against further bone loss induced by 10 µmol/l dexamethasone (Dex), simulating an osteoporosis (OP) model. TSG treatment (12.5 µg/ml) in Dex-induced zebrafish significantly increased the area of nodules by 50.14% compared with the untreated model group. In conclusion, TSG, as a major component of P. multiflorum Thunb. exhibited an osteogenic promoting activity in MSCs and in zebrafish. The results provided scientific evidence to support the potential use of TSG for protecting the bone in degenerative diseases, such as OP. PMID

  19. Tetrahydroxystilbene glucoside isolated fromPolygonum multiflorumThunb. demonstrates osteoblast differentiation promoting activity.

    Science.gov (United States)

    Zheng, Yayuan; Li, Jin; Wu, Jingkai; Yu, Yongjie; Yao, Weimin; Zhou, Manru; Tian, Jun; Zhang, Jingjing; Cui, Liao; Zeng, Xiaobin; Liu, Yuyu

    2017-10-01

    Polygonum multiflorum Thunb. is a traditional Chinese medicinal herb that has been widely used to treat age-associated diseases. Tetrahydroxystilbene glucoside (TSG), also known as 2,3,5,4-tetrahydroxystilbene-2-O-β-D-glucoside, is a major component of this herb. The present study was designed to investigate the osteogenic differentiation promoting activity of TSG in rat mesenchymal stem cells (MSCs) and in zebrafish. Preliminary experiments using MTT assay and ALP methods indicate that the high potential activity for promoting osteogenic differentiation was observed when 50% ethanol eluate was used. Further isolation and purification of TSG from the 50% ethanol eluate was performed by bioassay-guided fractionation, and its structure was confirmed using nuclear magnetic resonance and mass spectrometry analyses. In addition, the relative content of TSG with the highest potential activity in the promotion of osteogenic differentiation was identified as 14.34% by reversed-phase high performance liquid chromatography. Subsequently, the osteogenic differentiation promoting abilities of TSG in MSCs were examined. The results demonstrated that TSG promoted the alkaline phosphatase activity at concentrations of 1.56-25 µg/ml, while it increased the content of osteocalcin 7 days after treatment with 6.25-25 µg/ml in MSCs. Furthermore, experiments in zebrafish indicated that different concentrations of TSG (3.12-12.5 µg/ml) protected against further bone loss induced by 10 µmol/l dexamethasone (Dex), simulating an osteoporosis (OP) model. TSG treatment (12.5 µg/ml) in Dex-induced zebrafish significantly increased the area of nodules by 50.14% compared with the untreated model group. In conclusion, TSG, as a major component of P. multiflorum Thunb. exhibited an osteogenic promoting activity in MSCs and in zebrafish. The results provided scientific evidence to support the potential use of TSG for protecting the bone in degenerative diseases, such as OP.

  20. Demonstration in human plasma of a lectin activity analogous to that of bovine conglutinin

    DEFF Research Database (Denmark)

    Baatrup, G; Thiel, S; Isager, H

    1987-01-01

    Evidence of the existence in human plasma of an activity analogous to that of bovine conglutinin is presented. The human plasma component was characterized antigenically and functionally. Human plasma was shown to agglutinate complement-coated erythrocytes in the presence of Ca2+, and this conglu......Evidence of the existence in human plasma of an activity analogous to that of bovine conglutinin is presented. The human plasma component was characterized antigenically and functionally. Human plasma was shown to agglutinate complement-coated erythrocytes in the presence of Ca2......+, and this conglutination was inhibited by EDTA. The molecule also binds to complement-reacted solid phase IgG and to zymosan in the presence of Ca2+. The binding to complement is not inhibited by N-acetyl-D-mannosamine, but is inhibited by N-acetyl-D-glucosamine, as previously shown for bovine conglutinin. Antibodies...... raised against bovine conglutinin cross-react with the human protein. The plasma concentration of the conglutinin-like protein showed a high inter-individual variation between apparently healthy donors. Unlike bovine conglutinin, the human conglutinin activity could not be demonstrated in serum but only...

  1. Demonstration of in vitro antibacterial activity of the popular cosmetics items used by the Dhaka locality

    Directory of Open Access Journals (Sweden)

    Tanzia Akon

    2015-06-01

    Full Text Available Objective: To demonstrate the antibacterial activity of cosmetic products commonly used by the community of Dhaka metropolis. Methods: A total of 10 categories of cosmetic samples (with a subtotal of 30 brands were subjected to microbiological analysis through conventional culture and biochemical tests. Agar well diffusion method was used to determine the antibacterial trait in the tested samples which was further confirmed by the minimum inhibitory concentration method. Results: All samples were found to be populated with bacteria and fungi up to 105 CFU/ g and 103 CFU/g, respectively. Growth of Staphylococcus spp., Pseudomonas spp. and Klebsiella spp. was recorded as well. Conversely, 7 out of 30 items were found to exhibit the in vitro antibacterial activity against an array of laboratory test bacterial species including Staphylococcus spp., E. coli, Bacillus spp., Pseudomonas spp., Klebsiella spp. and Listeria spp. Consequently, all the samples showed antibacterial activity below the concentration of 0.46 mg/mL as found in the minimum inhibitory concentration test. Conclusions: Overall, the presence of huge microbial population in cosmetic products is not acceptable from the point microbiological contamination level. The antibacterial trait of these items, in contrary, may draw an overall public health impact.

  2. An investigation of the influence of reconceptualization of demonstrative experimental activities of optics in high school

    Directory of Open Access Journals (Sweden)

    Jair Lúcio Prados Ribeiro

    2013-08-01

    Full Text Available In this work, we analyze the influence that the use of demonstrative experiments can bring to the learning of optics. It is assumed that the development of experimental activities, when reconceptualized according to Hodson proposal, tends to contribute to the generation of cognitive conflicts when compared to traditional didactic experience. Justifications are given for an analysis of changes under a Piagetian bias, reconciled with Hodson proposal. The methodology used to structure the topics presentations was quasi-experimental, contrasting an experimental group with a control group. The measuring of the effectiveness of the suggested working method was made from a quantitative analysis, which identified some of the topics discussed had better results in learning, being more tied to the experiments carried out.

  3. The Japan Power Demonstration Reactor (JPDR) dismantling activities. Management of JPDR dismantling waste

    International Nuclear Information System (INIS)

    Abe, Masayoshi; Nakata, Susumu; Ito, Shinichi

    1996-01-01

    The management of wastes, both radioactive and non-radioactive, is one of the most important issues for a safe and reasonable dismantling operation of nuclear power plants. A large amount of radioactive wastes is arising from a reactor dismantling operation in a relatively short period time, ranging in a wide variety from very low level to relatively high level. Moreover non-radioactive waste is also in a huge amount. The dismantling operation of Japan Power Demonstration Reactor (JPDR) resulted in 24,440 tons of dismantling wastes, of which about 15% was radioactive and 85% non-radioactive. These wastes were managed successfully implementing a well developed management plan for JPDR dismantling waste. Research and development works for handling of JPDR dismantling wastes were performed, including fixation of loose contamination on surface, volume reduction and waste containers for on-site transportation and interim storage. The JPDR dismantling wastes generated were classified and categorized depending on their materials, characteristics and activity level. Approximately 2,100 tons of radioactive wastes were stored in the interim storage facilities on site using developed containers, and 1,670 tons of radioactive concrete waste were used for a safe demonstration test of a simple near-surface disposal for very low level waste. Other dismantling wastes such as steel and concrete which were categorized as non-radioactive were recycled and reused as useful resources. This paper describes the management of the JPDR dismantling wastes. (author)

  4. Long terminal repeat sequences from virulent and attenuated equine infectious anemia virus demonstrate distinct promoter activities.

    Science.gov (United States)

    Zhou, Tao; Yuan, Xiu-Fang; Hou, Shao-Hua; Tu, Ya-Bin; Peng, Jin-Mei; Wen, Jian-Xin; Qiu, Hua-Ji; Wu, Dong-Lai; Chen, Huan-Chun; Wang, Xiao-Jun; Tong, Guang-Zhi

    2007-09-01

    In the early 1970s, the Chinese Equine Infectious Anemia Virus (EIAV) vaccine, EIAV(DLA), was developed through successive passages of a wild-type virulent virus (EIAV(L)) in donkeys in vivo and then in donkey macrophages in vitro. EIAV attenuation and cell tropism adaptation are associated with changes in both envelope and long terminal repeat (LTR). However, specific LTR changes during Chinese EIAV attenuation have not been demonstrated. In this study, we compared LTR sequences from both virulent and attenuated EIAV strains and documented the diversities of LTR sequence from in vivo and in vitro infections. We found that EIAV LTRs of virulent strains were homologous, while EIAV vaccine have variable LTRs. Interestingly, experimental inoculation of EIAV(DLA) into a horse resulted in a restriction of the LTR variation. Furthermore, LTRs from EIAV(DLA) showed higher Tat transactivated activity than LTRs from virulent strains. By using chimeric clones of wild-type LTR and vaccine LTR, the main difference of activity was mapped to the changes of R region, rather than U3 region.

  5. Effect of a moderate-intensity demonstration walk on accuracy of physical activity self-report

    Directory of Open Access Journals (Sweden)

    Britta A. Larsen

    2017-06-01

    Conclusion: A 10-minute demonstration of MVPA was associated with reporting fewer minutes of MVPA and improved agreement with objective PA measures at baseline. These findings support combining self-report PA assessments with hands-on MVPA demonstrations.

  6. Demonstration of sulfur solubility determinations in high waste loading, low-activity waste glasses

    Energy Technology Data Exchange (ETDEWEB)

    Fox, K. M. [Savannah River Site (SRS), Aiken, SC (United States). Savannah River National Lab. (SRNL)

    2016-04-25

    A method recommended by Pacific Northwest National Laboratory (PNNL) for sulfate solubility determinations in simulated low-activity waste glasses was demonstrated using three compositions from a recent Hanford high waste loading glass study. Sodium and sulfate concentrations in the glasses increased after each re-melting step. Visual observations of the glasses during the re-melting process reflected the changes in composition. The measured compositions showed that the glasses met the targeted values. The amount of SO3 retained in the glasses after washing was relatively high, ranging from 1.6 to 2.6 weight percent (wt %). Measured SnO2 concentrations were notably low in all of the study glasses. The composition of the wash solutions should be measured in future work to determine whether SnO2 is present with the excess sulfate washed from the glass. Increases in batch size and the amount of sodium sulfate added did not have a measureable impact on the amount of sulfate retained in the glass, although this was tested for only a single glass composition. A batch size of 250 g and a sodium sulfate addition targeting 7 wt %, as recommended by PNNL, will be used in future experiments.

  7. Code Team Training: Demonstrating Adherence to AHA Guidelines During Pediatric Code Blue Activations.

    Science.gov (United States)

    Stewart, Claire; Shoemaker, Jamie; Keller-Smith, Rachel; Edmunds, Katherine; Davis, Andrew; Tegtmeyer, Ken

    2017-10-16

    Pediatric code blue activations are infrequent events with a high mortality rate despite the best effort of code teams. The best method for training these code teams is debatable; however, it is clear that training is needed to assure adherence to American Heart Association (AHA) Resuscitation Guidelines and to prevent the decay that invariably occurs after Pediatric Advanced Life Support training. The objectives of this project were to train a multidisciplinary, multidepartmental code team and to measure this team's adherence to AHA guidelines during code simulation. Multidisciplinary code team training sessions were held using high-fidelity, in situ simulation. Sessions were held several times per month. Each session was filmed and reviewed for adherence to 5 AHA guidelines: chest compression rate, ventilation rate, chest compression fraction, use of a backboard, and use of a team leader. After the first study period, modifications were made to the code team including implementation of just-in-time training and alteration of the compression team. Thirty-eight sessions were completed, with 31 eligible for video analysis. During the first study period, 1 session adhered to all AHA guidelines. During the second study period, after alteration of the code team and implementation of just-in-time training, no sessions adhered to all AHA guidelines; however, there was an improvement in percentage of sessions adhering to ventilation rate and chest compression rate and an improvement in median ventilation rate. We present a method for training a large code team drawn from multiple hospital departments and a method of assessing code team performance. Despite subjective improvement in code team positioning, communication, and role completion and some improvement in ventilation rate and chest compression rate, we failed to consistently demonstrate improvement in adherence to all guidelines.

  8. A preliminary demonstration of "virtual warehousing" and cross-docking technique with active RFID combined with asset tracking equipment.

    Science.gov (United States)

    2010-11-01

    The University of Denvers Intermodal Transportation Institute and System Planning : Corporations GlobalTrak system have successfully demonstrated the integration of GPS : tracking and active RFID monitoring of simulated cargo of pallet and cart...

  9. The management of high-level radioactive waste. A survey of demonstration activities

    International Nuclear Information System (INIS)

    1985-04-01

    The following aspects can be only indirectly demonstrated since they involve long periods of time. They have reached the degree of demonstration described below. Prediction of the physical stability of mined cavities at ambient temperatures is well understood from mining experience in salt and hard rocks such as granite. For soft rocks, the stability will depend upon the characteristics of the backfill and structural materials which can be included in predictive models. Normal and abnormal mechanisms for migration of radionuclides from repositories have been generally identified. Which of these mechanisms are relevant for the future evolution of a repository system depends on the repository site. Techniques for closure and sealing of repositories have been demonstrated, but validation of their long-term performance is lacking. The ability to construct mathematical models that predict repository and environmental behaviour over long periods of the future is now conceptually demonstrated. Some of these models are very sophisticated, but confidence is growing in their predictions. The reliability of results has been demonstrated, in some cases, by intercomparison of different modelling techniques; however, in many instances the models need validation. Generic data in support of models may, in many respects, be adequate. More data, and therefore R and D efforts concentrating on specific sites, are required to assist in the validation of some aspects of model predictions against the real world. This is an aspect of demonstration that continues to be necessary and is being pursued

  10. Demonstration of bacteriocin activity in bovine and bison strains of Pasteurella multocida. [UV radiation

    Energy Technology Data Exchange (ETDEWEB)

    Chengappa, M.M.; Carter, G.R.

    1977-08-01

    Of 33 strains of Pasteurella multocida examined, 14 showed bacteriocin activity and 17 were susceptible to bacteriocin. The activity was increased by about twofold if the cultures were induced with ultraviolet radiation; however, no increase in bacteriocin activity was observed if the potential producer strains were induced with mitomycin C. The bacteriocin activity of potential producer strains was increased if CaCl/sub 2/ was incorporated in the medium. The patterns of bacteriocin susceptibility indicate that these substances may ultimately contribute to a typing scheme for the species. An extra-chromosomal genetic element was not detected when a potential producer strain was not detected when a potential producer strain was tested by the dye-buoyant density gradient method. This fact suggests that the genetic material responsible for bacteriocin activity in P multocida is located on the host chromosome proper.

  11. Simultaneous demonstration of gelatinolytic activity, morphology, and immunohistochemical reaction using zymography film.

    Science.gov (United States)

    Kanomata, Naoki; Hasebe, Takahiro; Moriya, Takuya; Ochiai, Atsushi

    2013-12-01

    In situ zymography has been used to assess gelatinolytic activity, which is mainly due to matrix metalloproteinases (MMPs) in cancer tissues. MMPs play an important role in cancer invasion and metastasis. Film in situ zymography (FIZ) enables the in situ evaluation of gelatinolytic activity with high reproducibility. In this article, we report a study of FIZ, in a case of breast cancer with an invasive carcinoma component showing clear gelatinolytic activity, and in a non-invasive carcinoma component showing little gelatinolytic activity. Immunohistochemistry on FIZ was also performed. The simultaneous detection of gelatinolytic activity and immunohistochemical reaction was established in a single film. Immunohistochemistry on FIZ may have good potential for the investigation of cancer microenvironment.

  12. Studies on the oxidizing system in Holt's medium for histochemical demonstration of esterase activity

    DEFF Research Database (Denmark)

    Kirkeby, S; Blecher, S R

    1978-01-01

    Esterase activity in guinea-pig thyroid and mouse epididymis epithelial cells has been studied using 5-bromoindoxyl acetate as substrate. The pattern of esterase activity in the thyroid of the guinea-pig is constant, irrespective of whether ferri-ferrocyanide (FFC) or certain copper compounds...... cells contain an esterase activity which is not inhibited by conventional SH blocking agents, nor by high concentrations of FFC. From these results it appears that the mode of action of FFC in Holt's medium is as follows. At low concentrations FFC appears to act primarily as a catalytic agent...... in oxidation of indoxyl to indigoid. At high concentration FFC acts as an inhibitor of guinea-pig thyroid esterase, by oxidation of SH groups in the active centre. The esterase of mouse epididymis cell type EH 1 is not subject to this inhibition by FFC, presumably because it does not contain accessible SH...

  13. A demonstration of on-line plant corrosion monitoring using thin layer activation

    International Nuclear Information System (INIS)

    Asher, J.; Webb, J.W.; Wilkins, N.J.M.; Lawrence, P.F.; UKAEA Atomic Energy Research Establishment, Harwell. Materials Development Div.)

    1981-12-01

    The corrosion of a 1 inch water pipe in an evaporative cooling system has been monitored over three periods of plant operation using thin layer activation (TLA). The corrosion rate was followed at a sensitivity of about 1 μm and clearly reflected changes in plant operation. Examination of the test section after removal, both by autoradiography and metallography revealed the extent of corrosion and pitting over the active area. (author)

  14. Demonstration of ATP-dependent, ubiquitin-conjugating activities in higher plants

    International Nuclear Information System (INIS)

    Vierstra, R.D.

    1986-01-01

    Ubiquitin is a 76 amino acid eucaryotic polypeptide with several important functions that arise from its ability to become covalently ligated to other cytoplasmic and nuclear proteins. Ubiquitin has recently been purified from higher plants and found to be very homologous, both structurally and functionally, to the highly conserved animal form. Here, the authors present evidence that crude extracts from several plants have the capacity to conjugate ubiquitin to other plant proteins using either labelled human or oat ubiquitin as a substrate. The reaction requires ATP and can be detected in soluble extracts from dry seeds, etiolated shoots and green leaves, with etiolated shoot extracts having the highest activity. Mixing experiments indicate that the low activity found with green tissue in vitro is the result of an endogenous inhibitor. The conjugating activities are extremely labile with a half-life of 20 min at 30 0 C. The addition of polyphenol inhibitors fails to protect the system from this inactivation. In addition to conjugating activities, crude plant extracts also have ATP-independent activities that degrade ubiquitin conjugates. These results provide the first evidence that higher plants contain the necessary enzymes for ubiquitin conjugate formation. Further analysis of these activities should help clarify the functions of ubiquitination in plants

  15. The Lytic SA Phage Demonstrate Bactericidal Activity against Mastitis Causing Staphylococcus aureus

    Directory of Open Access Journals (Sweden)

    Hamza Ameer

    2016-01-01

    Full Text Available Staphylococcus aureus is the major causative agent of mastitis among dairy animals as it causes intramammary gland infection. Due to antibiotic resistance and contamination of antibiotics in the milk of diseased animals; alternative therapeutic agents are required to cure mastitis. Lytic bacteriophages and their gene products can be potential therapeutic agents against bacteria as they are host specific and less harmful than antibiotics. In this study, Staphylococcus aureus were isolated from milk samples of the infected animals and identified biochemically. SA phage was isolated from sewage water showing lytic activity against Staphylococcus aureus isolates. The highest lytic activity of bacteriophages was observed at 37°C and pH 7, and the most suitable storage condition was at 4°C. SA phage efficiently reduced bacterial growth in the bacterial reduction assay. The characterization and bacterial growth reduction activity of the bacteriophages against Staphylococcus aureus signifies their underlying potential of phage therapy against mastitis.

  16. Salvia leriifolia Benth (Lamiaceae) extract demonstrates in vitro antioxidant properties and cholinesterase inhibitory activity.

    Science.gov (United States)

    Loizzo, Monica R; Tundis, Rosa; Conforti, Filomena; Menichini, Federica; Bonesi, Marco; Nadjafi, Farsad; Frega, Natale Giuseppe; Menichini, Francesco

    2010-12-01

    The object of the present study was to investigate the in vitro antioxidant properties and cholinesterase inhibitory activity of Salvia leriifolia Benth extracts and fractions. The functional role of herbs and spices and their constituents is a hot topic in food-related plant research. Salvia species have been used since ancient times in folk medicine for cognitive brain function and have been subjected to extensive research. Thus, we hypothesize that S leriifolia, because of its functional properties, would be a good candidate to use as a nutraceutical product for improving memory in the elderly or patients affected by Alzheimer disease (ad). To test this hypothesis, we examined the cholinesterase inhibitory activity using the modified colorimetric Ellman's method against acetylcholinesterase (AChE) and butyrylcholinesterase (BChE). The n-hexane exhibited the highest activity, with inhibitory concentration 50% (IC(50)) values of 0.59 and 0.21 mg/mL, for AChE and BChE, respectively. This extract was fractionated, and 9 of these fractions (A-I) were obtained and tested. Fraction G, characterized by the presence of sesquiterpenes as major components, was the most active against AChE (IC(50) = 0.05 mg/mL). Because oxidative stress is a critical event in the pathogenesis of AD, we decided to screen the antioxidant activity (AA) using 2,2-diphenyl-1-picrylhydrazyl test, β-carotene bleaching test, and bovine brain peroxidation (thiobarbituric acid) assay. The ethyl acetate extract showed the highest activity, with IC(50) values of 2 and 33 μg/mL on β-carotene bleaching test and thiobarbituric acid test, respectively. These results suggest potential health benefits of S leriifolia extracts. However, this finding requires additional investigation in vivo. Copyright © 2010 Elsevier Inc. All rights reserved.

  17. Gaucher cells demonstrate a distinct macrophage phenotype and resemble alternatively activated macrophages

    NARCIS (Netherlands)

    Boven, Leonie A.; van Meurs, Marjan; Boot, Rolf G.; Mehta, Atul; Boon, Louis; Aerts, Johannes M.; Laman, Jon D.

    2004-01-01

    Although the existence of anti-inflammatory alternatively activated macrophages (aamphi) has been accepted widely based on in vitro studies, their in. vivo location, phenotype, and function still are debated. Gaucher disease (GD) is caused by a genetic deficiency in the lysosomal enzyme

  18. Of Heart & Kidneys: Hands-On Activities for Demonstrating Organ Function & Repair

    Science.gov (United States)

    Kao, Robert M.

    2014-01-01

    A major challenge in teaching organ development and disease is deconstructing a complex choreography of molecular and cellular changes over time into a linear stepwise process for students. As an entry toward learning developmental concepts, I propose two inexpensive hands-on activities to help facilitate learning of (1) how to identify defects in…

  19. Recovery of autonomic nervous activity after myocardial infarction demonstrated by short-term measurements of SDNN

    DEFF Research Database (Denmark)

    Vaage-Nilsen, M; Rasmussen, Verner; Jensen, Gorm Boje

    2001-01-01

    OBJECTIVE: Heart rate variability (HRV) has been demonstrated to be a risk factor after acute myocardial infarction (AMI). In the present study serial measurement of SDNN (standard deviation of the mean of qualified NN-interval) in short intervals was used to assess HRV changes after AMI......, and determine the role of these as independent risk factors compared to clinical, arrhythmic, ischemic and anamnestic variables. Measurements from a normal healthy middle-aged male population were used as reference (n = 63). METHODS: SDNN from a five-minute period during day and night-time, respectively...

  20. Recovery of autonomic nervous activity after myocardial infarction demonstrated by short-term measurements of SDNN

    DEFF Research Database (Denmark)

    Vaage-Nilsen, M; Rasmussen, Verner; Jensen, Gorm Boje

    2001-01-01

    OBJECTIVE: Heart rate variability (HRV) has been demonstrated to be a risk factor after acute myocardial infarction (AMI). In the present study serial measurement of SDNN (standard deviation of the mean of qualified NN-interval) in short intervals was used to assess HRV changes after AMI, and det......OBJECTIVE: Heart rate variability (HRV) has been demonstrated to be a risk factor after acute myocardial infarction (AMI). In the present study serial measurement of SDNN (standard deviation of the mean of qualified NN-interval) in short intervals was used to assess HRV changes after AMI......, and determine the role of these as independent risk factors compared to clinical, arrhythmic, ischemic and anamnestic variables. Measurements from a normal healthy middle-aged male population were used as reference (n = 63). METHODS: SDNN from a five-minute period during day and night-time, respectively......, was examined in 103 patients 1 week (n = 54), 1 month (n = 72) and 12-16 months (n = 54) after infarction. RESULTS: Day SDNN did not change during one-and-a-half years after AMI, and was significantly reduced compared with healthy males. Night SDNN, low after 1 week, with recovery 1 month after AMI...

  1. Mucus-stimulating activity in the sera of patients with cystic fibrosis: demonstration and preliminary fractionation.

    Science.gov (United States)

    Kurlandsky, L E; Berninger, R W; Talamo, R C

    1980-11-01

    The ciliated, mucus-secreting urn cell complex (UCC) is found swimming in the coelomic cavity of the marine invertebrate Sipunculus nudus. This cell complex, which can be maintained in suspension cultures, responds to various stimuli by hypersecreting mucus in the form of a cohesive mucus "tail." This tail can be measured and expressed as a multiple of "urn cell diameters." Using this bioassay, heated sera from 35 patients with cystic fibrosis (CF), 29 patients who were obligate heterozygotes for the CF gene, and 42 controls with a variety of diseases were tested. Control sera yielded a mean (+/- S.D.) mucus tail length of 2.5 (+/-2.3); CF sera yielded a mean mucus tail length of 7.5 (+/- 2.9), (P Sipunculus, as well as from different Sipunculi. In addition, sera from 3 CF patients and 3 controls were chromatographed on protein A-Sepharose. The bound IgG fraction was then washed with 8 M urea and subsequently eluted with 1 M acetic acid. Pooled dialyzed, lyophilized fractions were assayed as coded samples in the UCC assay. Mucus-stimulating activity as measured by mucus tail length per mg protein was greatest in the fractions eluted with 8 M urea. The 8 M urea fractions from 3 CF sera were 2.8 to 5.5 times as active as fractions from 3 control sera. The UCC assay can quantitatively measure mucus-stimulating activity in CF serum. This activity appears to be associated with a serum fraction which can be dissociated from IgG.

  2. Field Demonstration of Active Desiccant-Based Outdoor Air Preconditioning Systems, Final Report: Phase 3; FINAL

    International Nuclear Information System (INIS)

    Fischer, J.

    2001-01-01

    This report summarizes an investigation of the performance of two active desiccant cooling systems that were installed as pilot systems in two locations-a college dormitory and a research laboratory-during the fall of 1999. The laboratory system was assembled in the field from commercially available Trane air-handling modules combined with a standard total energy recovery module and a customized active desiccant wheel, both produced by SEMCO. The dormitory system was a factory-built, integrated system produced by SEMCO that included both active desiccant and sensible-only recovery wheels, a direct-fired gas regeneration section, and a pre-piped Trane heat pump condensing section. Both systems were equipped with direct digital control systems, complete with full instrumentation and remote monitoring capabilities. This report includes detailed descriptions of these two systems, installation details, samples of actual performance, and estimations of the energy savings realized. These pi lot sites represent a continuation of previous active desiccant product development research (Fischer, Hallstrom, and Sand 2000; Fischer 2000). Both systems performed as anticipated, were reliable, and required minimal maintenance. The dehumidification/total-energy-recovery hybrid approach was particularly effective in all respects. System performance showed remarkable improvement in latent load handling capability and operating efficiency compared with the original conventional cooling system and with the conventional system that remained in another, identical wing of the facility. The dehumidification capacity of the pilot systems was very high, the cost of operation was very low, and the system was cost-effective, offering a simple payback for these retrofit installations of approximately 5 to 6 years. Most important, the dormitory system resolved numerous indoor air quality problems in the dormitory by providing effective humidity control and increased, continuous ventilation air

  3. Professional Elites in "Classes" Societies

    NARCIS (Netherlands)

    S.J. Magala (Slawomir)

    2003-01-01

    textabstractModern European identity has been forged in class struggles between the French revolution and fall of the Berlin Wall, which fell twice. Once, with the rest of the city in May 1945, when a national socialist alternative to a modernizing mix of parliamentary democracy and market economy

  4. In vitro demonstration of anti-lipogenic activity in serum from obese rats

    International Nuclear Information System (INIS)

    Harris, R.B.S.; Martin, R.J.

    1986-01-01

    Studies with parabiosed rats provide evidence for a humoral factor, originating in obese animals, that specifically inhibits adipose lipogenesis. A bioassay was developed that allows serum from obese rats to be tested for this factor in vitro. Adipocytes are isolated from epididymal fat of 250g Sprague-Dawley rats. The cells are preincubated at 37 0 C for 1 or 12 hrs, in TC199 media containing 1.1 mg/ml glucose, 0.1 M Hepes and 2% serum. Following preincubation, the cells are washed 3 times and resuspended in serum-free media. Aliquots of cells are tested for metabolic activity in a subsequent 2 hour radiolabelled incubation in serum-free media with the addition of 0.5 μCi/ml U- 14 C-glucose. Basal, insulin (100 μU/ml) and norepinephrine (0.1 μg/ml) stimulated rates of glucose oxidation and conversion to triglyceride fatty acids are measured. Using serum from ad libitum fed rats as control, preincubation with serum from obese rats (20 days at 2 x normal intake) depressed basal and insulin stimulated glucose oxidation, and basal fatty acid synthesis. Serum from obese parabiotic rats and parabiotic partners of obese rats depressed basal fatty acid synthesis. This assay allows us to test serum for anti-lipogenic activity and may be used to identify the factor responsible for this activity in obese animals

  5. Recovery of autonomic nervous activity after myocardial infarction demonstrated by short-term measurements of SDNN

    DEFF Research Database (Denmark)

    Vaage-Nilsen, M; Rasmussen, Verner; Jensen, Gorm Boje

    2001-01-01

    OBJECTIVE: Heart rate variability (HRV) has been demonstrated to be a risk factor after acute myocardial infarction (AMI). In the present study serial measurement of SDNN (standard deviation of the mean of qualified NN-interval) in short intervals was used to assess HRV changes after AMI......, was significantly reduced compared with healthy males early, but not late after AMI. Thus, the study indicated during day-time a continuous abnormal sympathetic preponderance in the course of 16 months after AMI, and during night-time a gradual recovery of parasympathetic preponderance beginning early after AMI......, and determine the role of these as independent risk factors compared to clinical, arrhythmic, ischemic and anamnestic variables. Measurements from a normal healthy middle-aged male population were used as reference (n = 63). METHODS: SDNN from a five-minute period during day and night-time, respectively...

  6. Active vibration-based SHM system: demonstration on an operating Vestas V27 wind turbine

    DEFF Research Database (Denmark)

    Tcherniak, Dmitri; Mølgaard, Lasse Lohilahti

    2016-01-01

    with the system and a 3.5 month monitoring campaign was conducted while the turbine was operating normally. During the campaign, a defect – a trailing edge opening – was artificially introduced into the blade and its size was gradually increased from the original 15 cm to 45 cm. Using an unsupervised learning......This study presents a system that is able to detect defects like cracks, leading/trailing edge opening or delamination of at least 15 cm size, remotely, without stopping the wind turbine. The system is vibration-based: mechanical energy is artificially introduced by means of an electromechanical......-to-noise ratio. At the same time, the corresponding wavelength is short enough to deliver required damage detection resolution and long enough to be able to propagate the entire blade length. The paper demonstrates the system on a 225 kW Vesta s V27 wind turbine. One blade of the wind turbine was equipped...

  7. Cover Your Cough! A Short and Simple Activity to Demonstrate the Antimicrobial Effect of Desiccation

    Directory of Open Access Journals (Sweden)

    Jennifer Cook Easterwood

    2013-08-01

    Full Text Available Many undergraduate microbiology laboratory manuals include exercises demonstrating the antimicrobial effects of physical agents, such as UV light and heat, and chemical agents, such as disinfectants and antibiotics (3, 4. There is, however, a lack of exercises examining the effects of desiccation on bacterial growth and survival. This particular form of antimicrobial control is especially relevant today with an increased emphasis on coughing and sneezing into one’s sleeve or a tissue, where microbes will not contaminate hands and will eventually desiccate and die (2. Desiccation can have bacteriostatic or bactericidal effects depending on the species, the material on which the organism has desiccated, and the length of time. The absence of water can damage many cellular components, including enzymes, nucleic acids, and cell membranes (1. However, many prokaryotes have some degree of resistance to desiccation, with Escherichia coli surviving around 24 hours and Bacillus species surviving upwards of 300 years, though these numbers can vary due to a number of confounding factors (5. Some of these factors include the method by which desiccation occurred, whether desiccation occurred in a natural or laboratory situation, and the species itself (5. To address the effects of desiccation on bacterial growth and survival, a short, simple exercise was developed. By inoculating various materials with bacterial cultures and allowing them to air-dry for 24 hours, students can visualize the effects of desiccation by analyzing the growth, or lack thereof, when organisms are transferred to nutrient agar plates. This exercise has been used in a health professions microbiology course as well as a microbiology course for biology and biochemistry majors. It is short enough to be conducted during a standard lecture period or during a longer laboratory period in conjunction with other experiments demonstrating the effectiveness of physical agents on microbial

  8. Antiproliferative activity of Eremanthus crotonoides extracts and centratherin demonstrated in brain tumor cell lines

    Directory of Open Access Journals (Sweden)

    Jonathas F. R. Lobo

    2012-12-01

    Full Text Available The genus Eremanthus is recognized by the predominance of sesquiterpene lactones from the furanoheliangolide type, a class of substances extensively tested against cancer cell lines. Thus, the species E. crotonoides (DC. Sch. Bip., Asteraceae, obtained on "restinga" vegetation was evaluated against U251 and U87-MG glioma cell lines using the MTT colorimetric assay. Dichloromethane fraction was cytotoxic to both glioblastoma multiforme cell lines. We then conducted UPLC-PDA-ESI-MS/MS analysis of the dichloromethane fraction, which allowed the identification of the sesquiterpene lactones centratherin and goyazensolide. The isolation of centratherin was performed using chromatographic techniques and the identification of this substance was confirmed according to NMR data. Cytotoxic activity of centratherin alone was also evaluated against both U251 and U87-MG cells, which showed IC50 values comparable with those obtained for the commercial anticancer drug doxorubicin. All the tested samples showed cytotoxic activity against glioblastoma multiforme cells which suggests that E. crotonoides extracts may be important sources of antiproliferative substances and that the centratherin may serve as prototype for developing new antiglioblastoma drugs.

  9. Exploratory Characterization of Phenolic Compounds with Demonstrated Anti-Diabetic Activity in Guava Leaves at Different Oxidation States

    OpenAIRE

    D?az-de-Cerio, Elixabet; Verardo, Vito; G?mez-Caravaca, Ana Mar?a; Fern?ndez-Guti?rrez, Alberto; Segura-Carretero, Antonio

    2016-01-01

    Psidium guajava L. is widely used like food and in folk medicine all around the world. Many studies have demonstrated that guava leaves have anti-hyperglycemic and anti-hyperlipidemic activities, among others, and that these activities belong mainly to phenolic compounds, although it is known that phenolic composition in guava tree varies throughout seasonal changes. Andalusia is one of the regions in Europe where guava is grown, thus, the aim of this work was to study the phenolic compounds ...

  10. Using Wavelet Entropy to Demonstrate how Mindfulness Practice Increases Coordination between Irregular Cerebral and Cardiac Activities.

    Science.gov (United States)

    Sik, Hin Hung; Gao, Junling; Fan, Jicong; Wu, Bonnie Wai Yan; Leung, Hang Kin; Hung, Yeung Sam

    2017-05-10

    In both the East and West, traditional teachings say that the mind and heart are somehow closely correlated, especially during spiritual practice. One difficulty in proving this objectively is that the natures of brain and heart activities are quite different. In this paper, we propose a methodology that uses wavelet entropy to measure the chaotic levels of both electroencephalogram (EEG) and electrocardiogram (ECG) data and show how this may be used to explore the potential coordination between the mind and heart under different experimental conditions. Furthermore, Statistical Parametric Mapping (SPM) was used to identify the brain regions in which the EEG wavelet entropy was the most affected by the experimental conditions. As an illustration, the EEG and ECG were recorded under two different conditions (normal rest and mindful breathing) at the beginning of an 8-week standard Mindfulness-based Stress Reduction (MBSR) training course (pretest) and after the course (posttest). Using the proposed method, the results consistently showed that the wavelet entropy of the brain EEG decreased during the MBSR mindful breathing state as compared to that during the closed-eye resting state. Similarly, a lower wavelet entropy of heartrate was found during MBSR mindful breathing. However, no difference in wavelet entropy during MBSR mindful breathing was found between the pretest and posttest. No correlation was observed between the entropy of brain waves and the entropy of heartrate during normal rest in all participants, whereas a significant correlation was observed during MBSR mindful breathing. Additionally, the most well-correlated brain regions were located in the central areas of the brain. This study provides a methodology for the establishment of evidence that mindfulness practice (i.e., mindful breathing) may increase the coordination between mind and heart activities.

  11. Tocotrienol-Rich Fraction from Rice Bran Demonstrates Potent Radiation Protection Activity

    Directory of Open Access Journals (Sweden)

    Kimberly J. Krager

    2015-01-01

    Full Text Available The vitamin E analogs δ-tocotrienol (DT3 and γ-tocotrienol (GT3 have significant protective and mitigative capacity against the detrimental effects of ionizing radiation (IR. However, the expense of purification limits their potential use. This study examined the tocotrienol-rich fraction of rice bran (TRFRB isolated from rice bran deodorizer distillate, a rice oil refinement waste product, to determine its protective effects against IR induced oxidative damage and H2O2. Several cell lines were treated with tocotrienols or TRFRB prior to or following exposure to H2O2 or IR. To determine the radioprotective capacity cells were analyzed for morphology, mitochondrial bioenergetics, clonogenic survival, glutathione oxidation, cell cycle, and migration rate. TRFRB displayed similar antioxidant activity compared to pure tocotrienols. Cells pretreated with TRFRB or DT3 exhibited preserved cell morphology and mitochondrial respiration when exposed to H2O2. Oxidized glutathione was decreased in TRFRB treated cells exposed to IR. TRFRB reversed mitochondrial uncoupling and protected cells migration rates following IR exposure. The protective antioxidant capacity of TRFRB treated cells against oxidative injury was similar to that of purified DT3. TRFRB effectively protects normal cells against IR induced injury suggesting that rice bran distillate may be an inexpensive and abundant alternate source.

  12. An Active Learning Mammalian Skeletal Muscle Lab Demonstrating Contractile and Kinetic Properties of Fast- and Slow-Twitch Muscle

    Science.gov (United States)

    Head, S. I.; Arber, M. B.

    2013-01-01

    The fact that humans possess fast and slow-twitch muscle in the ratio of approximately 50% has profound implications for designing exercise training strategies for power and endurance activities. With the growth of exercise and sport science courses, we have seen the need to develop an undergraduate student laboratory that demonstrates the basic…

  13. Requirements and feasibility study of flight demonstration of Active Controls Technology (ACT) on the NASA 515 airplane

    Science.gov (United States)

    Gordon, C. K.

    1975-01-01

    A preliminary design study was conducted to evaluate the suitability of the NASA 515 airplane as a flight demonstration vehicle, and to develop plans, schedules, and budget costs for fly-by-wire/active controls technology flight validation in the NASA 515 airplane. The preliminary design and planning were accomplished for two phases of flight validation.

  14. Chitosan-propolis nanoparticle formulation demonstrates anti-bacterial activity against Enterococcus faecalis biofilms.

    Science.gov (United States)

    Ong, Teik Hwa; Chitra, Ebenezer; Ramamurthy, Srinivasan; Siddalingam, Rajinikanth Paruvathanahalli; Yuen, Kah Hay; Ambu, Stephen Periathamby; Davamani, Fabian

    2017-01-01

    Propolis obtained from bee hives is a natural substance with antimicrobial properties. It is limited by its insolubility in aqueous solutions; hence ethanol and ethyl acetate extracts of Malaysian propolis were prepared. Both the extracts displayed antimicrobial and anti-biofilm properties against Enterococcus faecalis, a common bacterium associated with hospital-acquired infections. High performance liquid chromatography (HPLC) analysis of propolis revealed the presence of flavonoids like kaempferol and pinocembrin. This study investigated the role of propolis developed into nanoparticles with chitosan for its antimicrobial and anti-biofilm properties against E. faecalis. Bacteria that grow in a slimy layer of biofilm are resistant to penetration by antibacterial agents. The use of nanoparticles in medicine has received attention recently due to better bioavailability, enhanced penetrative capacity and improved efficacy. A chitosan-propolis nanoformulation was chosen based on ideal physicochemical properties such as particle size, zeta potential, polydispersity index, encapsulation efficiency and the rate of release of the active ingredients. This formulation inhibited E. faecalis biofilm formation and reduced the number of bacteria in the biofilm by ~90% at 200 μg/ml concentration. When tested on pre-formed biofilms, the formulation reduced bacterial number in the biofilm by ~40% and ~75% at 200 and 300 μg/ml, respectively. The formulation not only reduced bacterial numbers, but also physically disrupted the biofilm structure as observed by scanning electron microscopy. Treatment of biofilms with chitosan-propolis nanoparticles altered the expression of biofilm-associated genes in E. faecalis. The results of this study revealed that chitosan-propolis nanoformulation can be deemed as a potential anti-biofilm agent in resisting infections involving biofilm formation like chronic wounds and surgical site infections.

  15. Chitosan-propolis nanoparticle formulation demonstrates anti-bacterial activity against Enterococcus faecalis biofilms.

    Directory of Open Access Journals (Sweden)

    Teik Hwa Ong

    Full Text Available Propolis obtained from bee hives is a natural substance with antimicrobial properties. It is limited by its insolubility in aqueous solutions; hence ethanol and ethyl acetate extracts of Malaysian propolis were prepared. Both the extracts displayed antimicrobial and anti-biofilm properties against Enterococcus faecalis, a common bacterium associated with hospital-acquired infections. High performance liquid chromatography (HPLC analysis of propolis revealed the presence of flavonoids like kaempferol and pinocembrin. This study investigated the role of propolis developed into nanoparticles with chitosan for its antimicrobial and anti-biofilm properties against E. faecalis. Bacteria that grow in a slimy layer of biofilm are resistant to penetration by antibacterial agents. The use of nanoparticles in medicine has received attention recently due to better bioavailability, enhanced penetrative capacity and improved efficacy. A chitosan-propolis nanoformulation was chosen based on ideal physicochemical properties such as particle size, zeta potential, polydispersity index, encapsulation efficiency and the rate of release of the active ingredients. This formulation inhibited E. faecalis biofilm formation and reduced the number of bacteria in the biofilm by ~90% at 200 μg/ml concentration. When tested on pre-formed biofilms, the formulation reduced bacterial number in the biofilm by ~40% and ~75% at 200 and 300 μg/ml, respectively. The formulation not only reduced bacterial numbers, but also physically disrupted the biofilm structure as observed by scanning electron microscopy. Treatment of biofilms with chitosan-propolis nanoparticles altered the expression of biofilm-associated genes in E. faecalis. The results of this study revealed that chitosan-propolis nanoformulation can be deemed as a potential anti-biofilm agent in resisting infections involving biofilm formation like chronic wounds and surgical site infections.

  16. Preclinical Pharmacokinetics and Pharmacodynamic Target of SCY-078, a First-in-Class Orally Active Antifungal Glucan Synthesis Inhibitor, in Murine Models of Disseminated Candidiasis.

    Science.gov (United States)

    Wring, Stephen A; Randolph, Ryan; Park, SeongHee; Abruzzo, George; Chen, Qing; Flattery, Amy; Garrett, Graig; Peel, Michael; Outcalt, Russell; Powell, Kendall; Trucksis, Michelle; Angulo, David; Borroto-Esoda, Katyna

    2017-04-01

    SCY-078 (MK-3118) is a novel, semisynthetic derivative of enfumafungin and represents the first compound of the triterpene class of antifungals. SCY-078 exhibits potent inhibition of β-(1,3)-d-glucan synthesis, an essential cell wall component of many pathogenic fungi, including Candida spp. and Aspergillus spp. SCY-078 is currently in phase 2 clinical development for the treatment of invasive fungal diseases. In vitro disposition studies to assess solubility, intestinal permeability, and metabolic stability were predictive of good oral bioavailability. Preclinical pharmacokinetic studies were consistent with once-daily administration to humans. After intravenous delivery, plasma clearance in rodents and dogs was low, representing candidiasis, exceeded plasma by 20- to 25-fold for the area under the concentration-time curve from 0 h to infinity (AUC 0-∞ ) and C max SCY-078 achieved efficacy endpoints following oral delivery across multiple murine models of disseminated candidiasis. The pharmacokinetic/pharmacodynamic indices C max /MIC and AUC/MIC correlated with outcome. Target therapeutic exposure, expressed as the plasma AUC 0-24 , was comparable across models, with an upper value of 11.2 μg·h/ml (15.4 μM·h); the corresponding mean value for free drug AUC/MIC was ∼0.75. Overall, these results demonstrate that SCY-078 has the oral and intravenous (i.v.) pharmacokinetic properties and potency in murine infection models of disseminated candidiasis to support further investigation as a novel i.v. and oral treatment for invasive fungal diseases. Copyright © 2017 Wring et al.

  17. Microbiological analysis of common preservatives used in food items and demonstration of their in vitro anti-bacterial activity

    Directory of Open Access Journals (Sweden)

    Tohora Sultana

    2014-12-01

    Full Text Available Objective: To quantify the microorganisms contaminating the common preservatives used in food as well as to detect their in vitro anti-bacterial traits. Methods: A total of 9 preservatives were subjected to conventional cultural and biochemical methods for microbial enumeration. Anti-bacterial activities were demonstrated through the agar well diffusion method. Results: All samples were found to be contaminated with bacteria up to 105 CFU/g and with the fungal flora within a range of 1 01-1 02 CFU/g. Escherichia coli, Pseudomonas spp. and Staphylococcus spp. were demonstrated in most of the samples. Sodium sulfite and citric acid possessed the strongest anti-bacterial trait against all of the test bacteria. Acetic acid exhibited activity against 6 out of 8 test bacteria while vinegar exhibited the activity against 4 bacteria. Activity of salt was demonstrated only against Listeria spp. and Bacillus spp., while activity of sugar and honey was found only against Escherichia coli and Klebsiella spp., respectively. Conclusions: According to the current investigation, sodium sulfite and citric acid samples were found to be satisfactory preservatives both in terms of microbiological criteria and their antibacterial traits.

  18. Comparison of antibacterial activity of glass-ionomer cement and amalgam in class two restorations by Streptococcus mutans count analysis at fixed intervals: an in vivo study.

    Science.gov (United States)

    Tegginmani, Veeresh S; Goel, Beenarani; Uppin, Virendra; Horatti, Priya; Kumar, L S Vijay; Nainani, Abhinav

    2013-05-01

    The purpose of the present study was to determine the influence of glass ionomer cement and amalgam restoration on the level of Streptococcus mutans in the interproximal plaque at periodic intervals and also to compare these values. Seventeen adult patients having two proximal carious lesions on any quadrant of the jaw (either opposing or contralateral) were selected for this study. Carious lesions were diagnosed clinically and from bitewing radiographs. Of the two carious lesions, one was restored with glass ionomer cermet cement and another with amalgam. Plaque samples were collected from interproximal areas before and at 1 month and 3 months post-treatment in a test tube containing 5 ml of modified Stuart's liquid transport fluid. Identification of organisms in the colony was done after Gram staining. Comparison of values before restoration and after restoration at 1 month interval showed a statistically significant decrease (ppp>0.05). Glass ionomer restorations have definite advantage over the amalgam, as the tunnel preparation is more conservative and fluoride release from the glass ionomer inhibits the growth of S. mutans in the plaque. Glass ionomer cement should be preferred over amalgam in conservatively prepared restorations as it reduces the microbial activities due to fluoride release.

  19. Novel bacterial metabolite merochlorin A demonstrates in vitro activity against multi-drug resistant methicillin-resistant Staphylococcus aureus.

    Directory of Open Access Journals (Sweden)

    George Sakoulas

    Full Text Available We evaluated the in vitro activity of a merochlorin A, a novel compound with a unique carbon skeleton, against a spectrum of clinically relevant bacterial pathogens and against previously characterized clinical and laboratory Staphylococcus aureus isolates with resistance to numerous antibiotics.Merochlorin A was isolated and purified from a marine-derived actinomycete strain CNH189. Susceptibility testing for merochlorin A was performed against previously characterized human pathogens using broth microdilution and agar dilution methods. Cytotoxicity was assayed in tissue culture assays at 24 and 72 hours against human HeLa and mouse sarcoma L929 cell lines.The structure of as new antibiotic, merochlorin A, was assigned by comprehensive spectroscopic analysis. Merochlorin A demonstrated in vitro activity against Gram-positive bacteria, including Clostridium dificile, but not against Gram negative bacteria. In S. aureus, susceptibility was not affected by ribosomal mutations conferring linezolid resistance, mutations in dlt or mprF conferring resistance to daptomycin, accessory gene regulator knockout mutations, or the development of the vancomycin-intermediate resistant phenotype. Merochlorin A demonstrated rapid bactericidal activity against MRSA. Activity was lost in the presence of 20% serum.The unique meroterpenoid, merochlorin A demonstrated excellent in vitro activity against S. aureus and C. dificile and did not show cross-resistance to contemporary antibiotics against Gram positive organisms. The activity was, however, markedly reduced in 20% human serum. Future directions for this compound may include evaluation for topical use, coating biomedical devices, or the pursuit of chemically modified derivatives of this compound that retain activity in the presence of serum.

  20. Pattern recognition on the structure-activity relationship of nano Pt-Ru catalysts: methodology and preliminary demonstration.

    Science.gov (United States)

    Lu, Qingye; Yang, Bo; Zhuang, Lin; Lu, Juntao

    2005-05-12

    The activity of nano Pt-Ru catalysts is a multivariate function of particle size, alloyed degree, oxide composition, and so forth. The monodependencies of catalytic activity on individual structure parameters (structure-activity relationship, SAR) are of great importance but, unfortunately, unobtainable in practical measurements. A pattern-recognition methodology is proposed for the first time to extract SAR information from all of the relative experimental data, which we hope will cast new light on the in-depth understanding of this important catalyst. As a preliminary demonstration, a multivariate linear regression and a generalized regression neural network were applied to analyze a small data set for methanol oxidation. It was found that both increasing the content of hydrous ruthenium oxides and decreasing the particle size would benefit the catalytic activity, whereas the effect of the Pt-Ru alloy degree turned out to be unremarkable.

  1. Activation of substantia gelatinosa by midbrain reticular stimulation demonstrated with 2-deoxyglucose in the rat spinal cord

    International Nuclear Information System (INIS)

    Gonzales-Lima, F.

    1986-01-01

    The autoradiographic ( 14 C)2-deoxyglucose (2-DG) method was used to map the descending effects of midbrain reticular stimulation on the rat cervical spinal cord. The stimulation evoked consistently a defensive 'freezing' reaction as well as a large and highly localized increase in 2-DG uptake in the substantia gelatinosa (SG)(Rexed laminae 2-3). No stimulus-induced changes in 2-DG uptake were produced in the other regions of the spinal cord. The findings represent the first anatomical demonstration of the activating effects of the spinal cord. The findings represent the first anatomical demonstration of the activating effects of midbrain reticular stimulation on the spinal cord. They also support the concept of an integrative role for the SG in descending reticular mechanisms at the spinal cord level. (author)

  2. An active learning mammalian skeletal muscle lab demonstrating contractile and kinetic properties of fast- and slow-twitch muscle.

    Science.gov (United States)

    Head, S I; Arber, M B

    2013-12-01

    The fact that humans possess fast- and slow-twitch muscle in the ratio of ∼50% has profound implications for designing exercise training strategies for power and endurance activities. With the growth of exercise and sport science courses, we have seen the need to develop an undergraduate student laboratory that demonstrates the basic properties of fast- and slow-twitch mammalian skeletal muscle. This laboratory illustrates the major differences in contractile properties and fatigue profiles exhibited by the two muscle types. Students compare and contrast twitch kinetics, fused tetanus characteristics, force-frequency relationships, and fatigue properties of fast- and slow-twitch muscles. Examples of results collected by students during class are used to illustrate the type of data collected and analysis performed. During the laboratory, students are encouraged to connect factual information from their skeletal muscle lectures to their laboratory findings. This enables student learning in an active fashion; in particular, the isolated muscle preparation demonstrates that much of what makes muscle fast or slow is myogenic and not the product of the nervous or circulatory systems. This has far-reaching implications for motor control and exercise behavior and therefore is a crucial element in exercise science, with its focus on power and endurance sport activities. To measure student satisfaction with this active learning technique, a questionnaire was administered after the laboratory; 96% of the comments were positive in their support of active versus passive learning strategies.

  3. A model for the training effects in swimming demonstrates a strong relationship between parasympathetic activity, performance and index of fatigue.

    Directory of Open Access Journals (Sweden)

    Sébastien Chalencon

    Full Text Available Competitive swimming as a physical activity results in changes to the activity level of the autonomic nervous system (ANS. However, the precise relationship between ANS activity, fatigue and sports performance remains contentious. To address this problem and build a model to support a consistent relationship, data were gathered from national and regional swimmers during two 30 consecutive-week training periods. Nocturnal ANS activity was measured weekly and quantified through wavelet transform analysis of the recorded heart rate variability. Performance was then measured through a subsequent morning 400 meters freestyle time-trial. A model was proposed where indices of fatigue were computed using Banister's two antagonistic component model of fatigue and adaptation applied to both the ANS activity and the performance. This demonstrated that a logarithmic relationship existed between performance and ANS activity for each subject. There was a high degree of model fit between the measured and calculated performance (R(2=0.84±0.14,p<0.01 and the measured and calculated High Frequency (HF power of the ANS activity (R(2=0.79±0.07, p<0.01. During the taper periods, improvements in measured performance and measured HF were strongly related. In the model, variations in performance were related to significant reductions in the level of 'Negative Influences' rather than increases in 'Positive Influences'. Furthermore, the delay needed to return to the initial performance level was highly correlated to the delay required to return to the initial HF power level (p<0.01. The delay required to reach peak performance was highly correlated to the delay required to reach the maximal level of HF power (p=0.02. Building the ANS/performance identity of a subject, including the time to peak HF, may help predict the maximal performance that could be obtained at a given time.

  4. A novel human model of the neurodegenerative disease GM1 gangliosidosis using induced pluripotent stem cells demonstrates inflammasome activation.

    Science.gov (United States)

    Son, Mi-Young; Kwak, Jae Eun; Seol, Binna; Lee, Da Yong; Jeon, Hyejin; Cho, Yee Sook

    2015-09-01

    GM1 gangliosidosis (GM1) is an inherited neurodegenerative disorder caused by mutations in the lysosomal β-galactosidase (β-gal) gene. Insufficient β-gal activity leads to abnormal accumulation of GM1 gangliosides in tissues, particularly in the central nervous system, resulting in progressive neurodegeneration. Here, we report an in vitro human GM1 model, based on induced pluripotent stem cell (iPSC) technology. Neural progenitor cells differentiated from GM1 patient-derived iPSCs (GM1-NPCs) recapitulated the biochemical and molecular phenotypes of GM1, including defective β-gal activity and increased lysosomes. Importantly, the characterization of GM1-NPCs established that GM1 is significantly associated with the activation of inflammasomes, which play a critical role in the pathogenesis of various neurodegenerative diseases. Specific inflammasome inhibitors potently alleviated the disease-related phenotypes of GM1-NPCs in vitro and in vivo. Our data demonstrate that GM1-NPCs are a valuable in vitro human GM1 model and suggest that inflammasome activation is a novel target pathway for GM1 drug development. Copyright © 2015 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.

  5. Periowave demonstrates bactericidal activity against periopathogens and leads to improved clinical outcomes in the treatment of adult periodontitis

    Science.gov (United States)

    Street, Cale N.; Andersen, Roger; Loebel, Nicolas G.

    2009-02-01

    Periodontitis affects half of the U.S. population over 50, and is the leading cause of tooth loss after 35. It is believed to be caused by growth of complex bacterial biofilms on the tooth surface below the gumline. Photodynamic therapy, a technology used commonly in antitumor applications, has more recently been shown to exhibit antimicrobial efficacy. We have demonstrated eradication of the periopathogens Porphyromonas gingivalis, Fusobacterium nucleatum, and Aggregatibacter actinomycetemcomitans in vitro using PeriowaveTM; a commercial photodisinfection system. In addition, several clinical studies have now demonstrated the efficacy of this treatment. A pilot study in the U.S. showed that 68% of patients treated with PeriowaveTM adjunctively to scaling and root planing (SRP) showed clinical attachment level increase of >1 mm, as opposed to 30% with SRP alone. In a subsequent larger study, a second PeriowaveTM treatment 6 weeks after initial treatment led to pocket depth improvements of >1.5 mm in 89% of patients. Finally, in the most recent multicenter, randomized, examiner-blinded study conducted on 121 subjects in Canada, PeriowaveTM treatment produced highly significant gains in attachment level (0.88 mm vs. 0.57 mm; p=0.003) and pocket depth (0.87 mm vs. 0.63 mm; p=0.01) as compared to SRP alone. In summary, PeriowaveTM demonstrated strong bactericidal activity against known periopathogens, and treatment of periodontitis using this system produced significantly better clinical outcomes than SRP alone. This, along with the absence of any adverse events in patients treated to date demonstrates that PDT is a safe and effective treatment for adult chronic periodontitis.

  6. Leptospira interrogans activation of peripheral blood monocyte glycolipoprotein demonstrated in whole blood by the release of IL-6

    Directory of Open Access Journals (Sweden)

    F. Dorigatti

    2005-06-01

    Full Text Available Glycolipoprotein (GLP from pathogenic serovars of Leptospira has been implicated in the pathogenesis of leptospirosis by its presence in tissues of experimental animals with leptospirosis, the inhibition of the Na,K-ATPase pump activity, and induced production of cytokines. The aims of the present study were to investigate the induction of IL-6 by GLP in peripheral blood mononuclear cells (PBMC and to demonstrate monocyte stimulation at the cellular level in whole blood from healthy volunteers. PBMC were stimulated with increasing concentrations (5 to 2500 ng/ml of GLP extracted from the pathogenic L. interrogans serovar Copenhageni, lipopolysaccharide (positive control or medium (negative control, and supernatants were collected after 6, 20/24, and 48 h, and kept at -80ºC until use. Whole blood was diluted 1:1 in RPMI medium and cultivated for 6 h, with medium, GLP and lipopolysaccharide as described above. Monensin was added after the first hour of culture. Supernatant cytokine levels from PBMC were measured by ELISA and intracellular IL-6 was detected in monocytes in whole blood cultures by flow-cytometry. Monocytes were identified in whole blood on the basis of forward versus side scatter parameters and positive reactions with CD45 and CD14 antibodies. GLP ( > or = 50 ng/ml-induced IL-6 levels in supernatants were detected after 6-h incubation, reaching a peak after 20/24 h. The percentage of monocytes staining for IL-6 increased with increasing GLP concentration. Thus, our findings show a GLP-induced cellular activation by demonstrating the ability of GLP to induce IL-6 and the occurrence of monocyte activation in whole blood at the cellular level.

  7. Bovine CCL28 Mediates Chemotaxis via CCR10 and Demonstrates Direct Antimicrobial Activity against Mastitis Causing Bacteria.

    Directory of Open Access Journals (Sweden)

    Kyler B Pallister

    Full Text Available In addition to the well characterized function of chemokines in mediating the homing and accumulation of leukocytes to tissues, some chemokines also exhibit potent antimicrobial activity. Little is known of the potential role of chemokines in bovine mammary gland health and disease. The chemokine CCL28 has previously been shown to play a key role in the homing and accumulation of IgA antibody secreting cells to the lactating murine mammary gland. CCL28 has also been shown to act as an antimicrobial peptide with activity demonstrated against a wide range of pathogens including bacteria, fungi and protozoans. Here we describe the cloning and function of bovine CCL28 and document the concentration of this chemokine in bovine milk. Bovine CCL28 was shown to mediate cellular chemotaxis via the CCR10 chemokine receptor and exhibited antimicrobial activity against a variety of bovine mastitis causing organisms. The concentration of bovine CCL28 in milk was found to be highly correlated with the lactation cycle. Highest concentrations of CCL28 were observed soon after parturition, with levels decreasing over time. These results suggest a potential role for CCL28 in the prevention/resolution of bovine mastitis.

  8. Demonstration of Active Power Controls by Utility-Scale PV Power Plant in an Island Grid: Preprint

    Energy Technology Data Exchange (ETDEWEB)

    Gevorgian, Vahan; O' Neill, Barbara

    2017-02-01

    The National Renewable Energy Laboratory (NREL), AES, and the Puerto Rico Electric Power Authority conducted a demonstration project on a utility-scale photovoltaic (PV) plant to test the viability of providing important ancillary services from this facility. As solar generation increases globally, there is a need for innovation and increased operational flexibility. A typical PV power plant consists of multiple power electronic inverters and can contribute to grid stability and reliability through sophisticated 'grid-friendly' controls. In this way, it may mitigate the impact of its variability on the grid and contribute to important system requirements more like traditional generators. In 2015, testing was completed on a 20-MW AES plant in Puerto Rico, and a large amount of test data was produced and analyzed that demonstrates the ability of PV power plants to provide various types of new grid-friendly controls. This data showed how active power controls can leverage PV's value from being simply an intermittent energy resource to providing additional ancillary services for an isolated island grid. Specifically, the tests conducted included PV plant participation in automatic generation control, provision of droop response, and fast frequency response.

  9. Tested Demonstrations.

    Science.gov (United States)

    Gilbert, George L., Ed.

    1990-01-01

    Presented are two demonstrations; "Heat of Solution and Colligative Properties: An Illustration of Enthalpy and Entropy," and "A Vapor Pressure Demonstration." Included are lists of materials and experimental procedures. Apparatus needed are illustrated. (CW)

  10. Tested Demonstrations.

    Science.gov (United States)

    Gilbert, George L., Ed.

    1978-01-01

    Presents two demonstrations; one on Boyle's Law, to illustrate the gas law and serve as a challenging problem for the students; the other is a modified Color Blind Traffic Light demonstration in which the oscillating reactions were speeded up. (GA)

  11. Identification of peptides in wheat germ hydrolysate that demonstrate calmodulin-dependent protein kinase II inhibitory activity.

    Science.gov (United States)

    Kumrungsee, Thanutchaporn; Akiyama, Sayaka; Guo, Jian; Tanaka, Mitsuru; Matsui, Toshiro

    2016-12-15

    Hydrolysis of wheat germ by proteases resulted in bioactive peptides that demonstrated an inhibitory effect against the vasoconstrictive Ca(2+)-calmodulin (CaM)-dependent protein kinase II (CaMK II). The hydrolysate by thermolysin (1.0wt%, 5h) showed a particularly potent CaMK II inhibition. As a result of mixed mode high-performance liquid chromatography of thermolysin hydrolysate with pH elution gradient ranging between 4.8 and 8.9, the fraction eluted at pH 8.9 was the most potent CaMK II inhibitor. From this fraction, Trp-Val and Trp-Ile were identified as CaMK II inhibitors. In Sprague-Dawley rats, an enhanced aortic CaMK II activity by 1μM phenylephrine was significantly (p<0.05) suppressed by 15-min incubation with 300μM Trp-Val or Trp-Ile. On the basis of Ca(2+)-chelating fluorescence and CaMK II activity assays, it was concluded that Trp-Val and Trp-Ile competed with Ca(2+)-CaM complex to bind to CaMK II with Ki values of 5.4 and 3.6μM, respectively. Copyright © 2016 Elsevier Ltd. All rights reserved.

  12. Exploratory Characterization of Phenolic Compounds with Demonstrated Anti-Diabetic Activity in Guava Leaves at Different Oxidation States

    Science.gov (United States)

    Díaz-de-Cerio, Elixabet; Verardo, Vito; Gómez-Caravaca, Ana María; Fernández-Gutiérrez, Alberto; Segura-Carretero, Antonio

    2016-01-01

    Psidium guajava L. is widely used like food and in folk medicine all around the world. Many studies have demonstrated that guava leaves have anti-hyperglycemic and anti-hyperlipidemic activities, among others, and that these activities belong mainly to phenolic compounds, although it is known that phenolic composition in guava tree varies throughout seasonal changes. Andalusia is one of the regions in Europe where guava is grown, thus, the aim of this work was to study the phenolic compounds present in Andalusian guava leaves at different oxidation states (low, medium, and high). The phenolic compounds in guava leaves were determined by HPLC-DAD-ESI-QTOF-MS. The results obtained by chromatographic analysis reported that guava leaves with low degree of oxidation had a higher content of flavonols, gallic, and ellagic derivatives compared to the other two guava leaf samples. Contrary, high oxidation state guava leaves reported the highest content of cyanidin-glucoside that was 2.6 and 15 times higher than guava leaves with medium and low oxidation state, respectively. The QTOF platform permitted the determination of several phenolic compounds with anti-diabetic properties and provided new information about guava leaf phenolic composition that could be useful for nutraceutical production. PMID:27187352

  13. Exploratory Characterization of Phenolic Compounds with Demonstrated Anti-Diabetic Activity in Guava Leaves at Different Oxidation States.

    Science.gov (United States)

    Díaz-de-Cerio, Elixabet; Verardo, Vito; Gómez-Caravaca, Ana María; Fernández-Gutiérrez, Alberto; Segura-Carretero, Antonio

    2016-05-11

    Psidium guajava L. is widely used like food and in folk medicine all around the world. Many studies have demonstrated that guava leaves have anti-hyperglycemic and anti-hyperlipidemic activities, among others, and that these activities belong mainly to phenolic compounds, although it is known that phenolic composition in guava tree varies throughout seasonal changes. Andalusia is one of the regions in Europe where guava is grown, thus, the aim of this work was to study the phenolic compounds present in Andalusian guava leaves at different oxidation states (low, medium, and high). The phenolic compounds in guava leaves were determined by HPLC-DAD-ESI-QTOF-MS. The results obtained by chromatographic analysis reported that guava leaves with low degree of oxidation had a higher content of flavonols, gallic, and ellagic derivatives compared to the other two guava leaf samples. Contrary, high oxidation state guava leaves reported the highest content of cyanidin-glucoside that was 2.6 and 15 times higher than guava leaves with medium and low oxidation state, respectively. The QTOF platform permitted the determination of several phenolic compounds with anti-diabetic properties and provided new information about guava leaf phenolic composition that could be useful for nutraceutical production.

  14. Exploratory Characterization of Phenolic Compounds with Demonstrated Anti-Diabetic Activity in Guava Leaves at Different Oxidation States

    Directory of Open Access Journals (Sweden)

    Elixabet Díaz-de-Cerio

    2016-05-01

    Full Text Available Psidium guajava L. is widely used like food and in folk medicine all around the world. Many studies have demonstrated that guava leaves have anti-hyperglycemic and anti-hyperlipidemic activities, among others, and that these activities belong mainly to phenolic compounds, although it is known that phenolic composition in guava tree varies throughout seasonal changes. Andalusia is one of the regions in Europe where guava is grown, thus, the aim of this work was to study the phenolic compounds present in Andalusian guava leaves at different oxidation states (low, medium, and high. The phenolic compounds in guava leaves were determined by HPLC-DAD-ESI-QTOF-MS. The results obtained by chromatographic analysis reported that guava leaves with low degree of oxidation had a higher content of flavonols, gallic, and ellagic derivatives compared to the other two guava leaf samples. Contrary, high oxidation state guava leaves reported the highest content of cyanidin-glucoside that was 2.6 and 15 times higher than guava leaves with medium and low oxidation state, respectively. The QTOF platform permitted the determination of several phenolic compounds with anti-diabetic properties and provided new information about guava leaf phenolic composition that could be useful for nutraceutical production.

  15. Neuronal activation by electrical neuromuscular stimulation in hemiplegic patients demonstrated with 99m-Tc-ECD brain SPECT

    International Nuclear Information System (INIS)

    Anjos, D.A.; Santos, A.O.; Etchebehere, E.C.S.C.; Filho, R.M.; Ramos, C.D.; Lima, M.C.L.; Camargo, E.E.; Honorato, D.C.; Oberg, T.D.

    2002-01-01

    Electrical neuromuscular stimulation (ENS) has been shown to improve volitional movement of upper limbs and decrease muscle hypertonia in hemiplegic patients. Aim: The purpose of this study was to demonstrate regional cerebral perfusion changes secondary to neuronal activation after ENS using 99m Tc-ECD SPECT and to correlate these findings with clinical improvement. Materials and Methods: Nine hemiplegic and 3 paraparetic patients, with 14 to 59 years of age, 10 males and 2 females, were studied. ENS was performed for 14 weeks in 45-minute sessions on the muscles involved in hand opening and closing. Each patient was submitted to neurological examination before and after treatment and underwent three 99m Tc-ECD SPECT studies: a pre-treatment study; a study performed during the first ENS session; and the third study during the last ENS session (after 14 weeks of treatment). Visual analysis of brain SPECT images was performed by two experienced nuclear physicians. Region-to-pons ratio (R/PO) was obtained for 15 brain regions. An asymmetry index (AI) was also calculated for all regions using the equation: AI=2X(R-L)/(R+L), where R is right and L is left. The visual and semi-quantitative results were compared in the three studies. Results: Visual analysis revealed perfusion improvement mainly in areas adjacent to the brain lesion (penumbra) but also in the contra-lateral cerebral hemisphere. Perfusion improvement was found in the frontal lobe (5 patients), fronto-parietal (1), fronto-temporal (1), temporal (2), basal ganglia (5) and in the thalami (1). In the pre-treatment study, 8 patients showed cerebellar diaschisis, which decreased during treatment in 2 patients and increased in 2. The asymmetry index showed significant variability among the three studies in 8 regions. The R/PO ratio did not correlate with the visual analysis. Neurological examination showed significant improvement in 10 patients, 9 of which showed perilesional brain perfusion improvement

  16. What's Going on in Your Professor's Head? Demonstrating the Id, Ego, and Superego

    Science.gov (United States)

    Segrist, Dan J.

    2009-01-01

    This article describes an in-class activity designed to demonstrate Freud's structural theory of the psyche, specifically the roles of the id, ego, and superego, as well as the interplay among them. Additionally, the activity visually illustrates Freud's ideas about the levels of consciousness associated with these 3 components. Pre-post quiz…

  17. Methylmercury intoxication and histochemical demonstration of NADPH-diaphorase activity in the striate cortex of adult cats

    Directory of Open Access Journals (Sweden)

    R.B. Oliveira

    1998-09-01

    Full Text Available The effects of methylmercury (MeHg on histochemical demonstration of the NADPH-diaphorase (NADPH-d activity in the striate cortex were studied in 4 adult cats. Two animals were used as control. The contaminated animals received 50 ml milk containing 0.42 µg MeHg and 100 g fish containing 0.03 µg MeHg daily for 2 months. The level of MeHg in area 17 of intoxicated animals was 3.2 µg/g wet weight brain tissue. Two cats were perfused 24 h after the last dose (group 1 and the other animals were perfused 6 months later (group 2. After microtomy, sections were processed for NADPHd histochemistry procedures using the malic enzyme method. Dendritic branch counts were performed from camera lucida drawings for control and intoxicated animals (N = 80. Average, standard deviation and Student t-test were calculated for each data group. The concentrations of mercury (Hg in milk, fish and brain tissue were measured by acid digestion of samples, followed by reduction of total Hg in the digested sample to metallic Hg using stannous chloride followed by atomic fluorescence analysis. Only group 2 revealed a reduction of the neuropil enzyme activity and morphometric analysis showed a reduction in dendritic field area and in the number of distal dendrite branches of the NADPHd neurons in the white matter (P<0.05. These results suggest that NADPHd neurons in the white matter are more vulnerable to the long-term effects of MeHg than NADPHd neurons in the gray matter.

  18. Active optics and modified-Rumsey wide-field telescopes: MINITRUST demonstrators with vase- and tulip-form mirrors

    Science.gov (United States)

    Lemaître, Gérard R.; Montiel, Pierre; Joulié, Patrice; Dohlen, Kjetil; Lanzoni, Patrick

    2005-12-01

    Wide-field astronomy requires the development of larger aperture telescopes. The optical properties of a three-mirror modified-Rumsey design provide significant advantages when compared to other telescope designs: (i) at any wavelength, the design has a flat field and is anastigmatic; (ii) the system is extremely compact, i.e., it is almost four times shorter than a Schmidt. Compared to the equally compact flat-field Ritchey-Chrétien with a doublet-lens corrector, as developed for the Sloan digital sky survey - and which requires the polishing of six optical surfaces - the proposed modified-Rumsey design requires only a two-surface polishing and provides a better imaging quality. All the mirrors are spheroids of the hyperboloid type. Starting from the classical Rumsey design, it is shown that the use of all eight available free parameters allows the simultaneous aspherization of the primary and tertiary mirrors by active optics methods from a single deformable substrate. The continuity conditions between the primary and the tertiary hyperbolizations are achieved by an intermediate narrow ring of constant thickness that is not optically used. After the polishing of a double vase form in a spherical shape, the primary-tertiary hyperbolizations are achieved by in situ stressing. The tulip-form secondary is hyperbolized by stress polishing. Other active optics alternatives are possible for a space telescope. The modified-Rumsey design is of interest for developing large space- and ground-based survey telescopes in UV, visible, or IR ranges, such as currently demonstrated with the construction of identical telescopes MINITRUST-1 and -2, f/5 - 2° field of view. Double-pass optical tests show diffraction-limited images.

  19. Tested Demonstrations.

    Science.gov (United States)

    Gilbert, George L., Ed.

    1986-01-01

    Provides three descriptions of demonstrations used in various chemistry courses. Includes the use of a simple demonstration model to illustrate principles of chromatography, techniques for using balloons to teach about the behavior of gases, and the use of small concentrations of synthetic polyelectrolytes to induce the flocculation hydrophobic…

  20. A novel Smac mimetic APG-1387 demonstrates potent antitumor activity in nasopharyngeal carcinoma cells by inducing apoptosis.

    Science.gov (United States)

    Li, Ning; Feng, Lin; Han, Hui-Qiong; Yuan, Jing; Qi, Xue-Kang; Lian, Yi-Fan; Kuang, Bo-Hua; Zhang, Yu-Chen; Deng, Cheng-Cheng; Zhang, Hao-Jiong; Yao, You-Yuan; Xu, Miao; He, Gui-Ping; Zhao, Bing-Chun; Gao, Ling; Feng, Qi-Sheng; Chen, Li-Zhen; Yang, Lu; Yang, Dajun; Zeng, Yi-Xin

    2016-10-10

    Despite advances in the development of radiation against nasopharyngeal carcinoma (NPC), the management of advanced NPC remains a challenge. Smac mimetics are designed to neutralize inhibitor of apoptosis (IAP) proteins, thus reactivating the apoptotic program in cancer cells. In this study, we investigated the effect of a novel bivalent Smac mimetic APG-1387 in NPC. In vitro, APG-1387 in combination with TNF-α potently decreased NPC cell viability by inducing apoptosis in majority of NPC cell lines. The in vitro antitumor effect was RIPK1-dependent, whereas it was independent on IAPs, USP11, or EBV. Of note, the inhibition of NF-κB or AKT pathway rendered resistant NPC cells responsive to the treatment of APG-1387/TNF-α. In vivo, APG-1387 displayed antitumor activity as a single agent at well-tolerated doses, even in an in vitro resistant cell line. In summary, our results demonstrate that APG-1387 exerts a potent antitumor effect on NPC. These findings support clinical evaluation of APG-1387 as a potential treatment for advanced NPC. Copyright © 2016. Published by Elsevier Ireland Ltd.

  1. HOW E-LEARNING DEMONSTRATES THE FORMATION OF STUDENTS' COGNITIVE ACTIVITY IN THE TEACHING OF QUANTUM PHYSICS

    Directory of Open Access Journals (Sweden)

    Ihor V. Korsun

    2017-10-01

    Full Text Available The aim of this article is to prove the advisability of using the e-learning of quantum physics in the Moodle environment to help students achieve better success in this difficult subject area. The possibilities of Moodle in a distance learning environment have been analysed. E-learning tool of quantum physics in the Moodle environment has been described, and its educational opportunities have been determined. The need for material models and thought models for teaching of quantum physics has been proven. Modeling method and thought experiments explain phenomena of physics help to better understand real experiments and the essence of physics theories. The method of creation of computer models using Easy Gif Animator has been discussed. The requirements for material models have been identified, and an example of material model of Large Hadron Collider has been demonstrated. Results showed that e-learning of quantum physics increases the level of students' cognitive activity. This technique can be used for teaching other sections of physics and other natural sciences.

  2. Cycloartane-3,24,25-triol inhibits MRCKα kinase and demonstrates promising anti prostate cancer activity in vitro

    Directory of Open Access Journals (Sweden)

    Lowe Henry I C

    2012-11-01

    Full Text Available Abstract Background Given the high occurrence of prostate cancer worldwide and one of the major sources of the discovery of new lead molecules being medicinal plants, this research undertook to investigate the possible anti-cancer activity of two natural cycloartanes; cycloartane-3,24,25-diol (extracted in our lab from Tillandsia recurvata and cycloartane-3,24,25-triol (purchased. The inhibition of MRCKα kinase has emerged as a potential solution to restoring the tight regulation of normal cellular growth, the loss of which leads to cancer cell formation. Methods Kinase inhibition was investigated using competition binding (to the ATP sites assays which have been previously established and authenticated and cell proliferation was measured using the WST-1 assay. Results Cycloartane-3,24,25-triol demonstrated strong selectivity towards the MRCKα kinase with a Kd50 of 0.26 μM from a total of 451 kinases investigated. Cycloartane-3,24,25-triol reduced the viability of PC-3 and DU145 cell lines with IC50 values of 2.226 ± 0.28 μM and 1.67 ± 0.18 μM respectively. Conclusions These results will prove useful in drug discovery as Cycloartane-3,24,25-triol has shown potential for development as an anti-cancer agent against prostate cancer.

  3. Tested Demonstrations.

    Science.gov (United States)

    Gilbert, George L., Ed.

    1987-01-01

    Presents three demonstrations suitable for undergraduate chemistry classes. Focuses on experiments with calcium carbide, the induction by iron of the oxidation of iodide by dichromate, and the classical iodine clock reaction. (ML)

  4. RADIOACTIVE DEMONSTRATIONS OF FLUIDIZED BED STEAM REFORMING WITH ACUTAL HANFORD LOW ACTIVITY WASTES VERIFYING FBSR AS A SUPPLEMENTARY TREATMENT

    Energy Technology Data Exchange (ETDEWEB)

    Jantzen, C.; Crawford, C.; Burket, P.; Bannochie, C.; Daniel, G.; Nash, C.; Cozzi, A.; Herman, C.

    2012-01-12

    The U.S. Department of Energy's Office of River Protection is responsible for the retrieval, treatment, immobilization, and disposal of Hanford's tank waste. Currently there are approximately 56 million gallons of highly radioactive mixed wastes awaiting treatment. A key aspect of the River Protection Project cleanup mission is to construct and operate the Waste Treatment and Immobilization Plant (WTP). The WTP will separate the tank waste into high-level waste (HLW) and low-activity waste (LAW) fractions, both of which will subsequently be vitrified. The projected throughput capacity of the WTP LAW Vitrification Facility is insufficient to complete the cleanup mission in the time frame required by the Hanford Federal Facility Agreement and Consent Order, also known as the Tri-Party Agreement (TPA). Therefore, Supplemental Treatment is required both to meet the TPA treatment requirements as well as to more cost effectively complete the tank waste treatment mission. Fluidized Bed Steam Reforming (FBSR) is one of the supplementary treatments being considered. FBSR offers a moderate temperature (700-750 C) continuous method by which LAW and other secondary wastes can be processed irrespective of whether they contain organics, nitrates/nitrites, sulfates/sulfides, chlorides, fluorides, and/or radio-nuclides like I-129 and Tc-99. Radioactive testing of Savannah River LAW (Tank 50) shimmed to resemble Hanford LAW and actual Hanford LAW (SX-105 and AN-103) have produced a ceramic (mineral) waste form which is the same as the non-radioactive waste simulants tested at the engineering scale. The radioactive testing demonstrated that the FBSR process can retain the volatile radioactive components that cannot be contained at vitrification temperatures. The radioactive and nonradioactive mineral waste forms that were produced by co-processing waste with kaolin clay in an FBSR process are shown to be as durable as LAW glass.

  5. Demonstration projects

    International Nuclear Information System (INIS)

    Robertson, R.F.S.

    1976-01-01

    A discussion of some of the projects in Canada planned to demonstrate the technical and economic feasibility of processes using waste heat. Most of the studies are in the planning stage; few field demonstrations are yet in operation in any of the three arbitrary categories of waste heat - high-grade heat (100-200 deg C), medium-grade heat (30-100 deg C), and low-grade heat (below 30 deg C). The survey indicates that, while there is long-term potential in several of the approaches, the time has arrived to start finding some hard facts. (author)

  6. Field Demonstration of Active Desiccant Modules Designed to Integrate with Standard Unitary Rooftop Package Equipment - Final Report: Phase 3

    Energy Technology Data Exchange (ETDEWEB)

    Fischer, J

    2004-03-15

    This report summarizes the investigation of two active desiccant module (ADM) pilot site installations initiated in 2001. Both pilot installations were retrofits at existing facilities served by conventional heating, ventilating, and air-conditioning (HVAC) systems that had encountered frequent humidity control, indoor air quality (IAQ), and other operational problems. Each installation involved combining a SEMCO, Inc., ADM (as described in Fischer and Sand 2002) with a standard packaged rooftop unit built by the Trane Company. A direct digital control (DDC) system integral to the ADM performed the dual function of controlling the ADM/rooftop combination and facilitating data collection, trending, and remote performance monitoring. The first installation involved providing preconditioned outdoor air to replace air exhausted from the large kitchen hood and bathrooms of a Hooters restaurant located in Rome, Georgia. This facility had previously added an additional rooftop unit in an attempt to achieve occupant comfort without success. The second involved conditioning the outdoor air delivered to each room of a wing of the Mountain Creek Inn at the Callaway Gardens resort. This hotel, designed in the ''motor lodge'' format with each room opening to the outdoors, is located in southwest Georgia. Controlling the space humidity always presented a serious challenge. Uncomfortable conditions and musty odors had caused many guests to request to move to other areas within the resort. This is the first field demonstration performed by Oak Ridge National Laboratory where significant energy savings, operating cost savings, and dramatically improved indoor environmental conditions can all be claimed as the results of a retrofit desiccant equipment field installation. The ADM/rooftop combination installed at the restaurant resulted in a reduction of about 34% in the electricity used by the building's air-conditioning system. This represents a reduction of

  7. Polarized Light Corridor Demonstrations.

    Science.gov (United States)

    Davies, G. R.

    1990-01-01

    Eleven demonstrations of light polarization are presented. Each includes a brief description of the apparatus and the effect demonstrated. Illustrated are strain patterns, reflection, scattering, the Faraday Effect, interference, double refraction, the polarizing microscope, and optical activity. (CW)

  8. ICT Demonstration

    DEFF Research Database (Denmark)

    Jensen, Tine Wirenfeldt; Bay, Gina

    , formulating a research problem as well as writing techniques as deeply integrated elements of the academic work process. The importance of creating an engaging and supportive learning environment: When teaching subjects such as plagiarism and study skills, the student can easily be constructed as a cheater...... and a poor student by discourse that emphasizes rule-breaking or which highlights poor study skills rather than focusing on knowledge building. This view of the student as “untrustworthy” (McGregor, 1960) can be communicated not only through explicit discourse; it can be implicit in the design of learning......In this demonstration we present and discuss two interrelated on-line learning resources aimed at supporting international students at Danish universities in building study skills (the Study Metro) and avoiding plagiarism (Stopplagiarism). We emphasize the necessity of designing online learning...

  9. 77 FR 37059 - Draft Guidance for Industry on Active Controls in Studies To Demonstrate Effectiveness of a New...

    Science.gov (United States)

    2012-06-20

    ... advantages and disadvantages of using a study with an active control. Examples are provided to illustrate... with access to the Internet may obtain the draft guidance at either http://www.fda.gov/AnimalVeterinary...

  10. Community-based physical activity intervention using principles of social marketing: a demonstration project in Southern India.

    Science.gov (United States)

    Subitha, L; Soudarssanane, M Bala; Murugesan, R

    2013-01-01

    We aimed to study the development and implementation of promotion of physical activity in a rural community by applying the principles of social marketing and to determine participation behaviour in a physical activity programme in a community setting. The intervention targeted 485 people, 20-49 years of age, residents of Periakattupalayam and Rangareddipalayam villages, Tamil Nadu. This community-based participatory research was based on the principles of 'social marketing'. Health education by one-to-one counselling, written materials and community events were used to popularize moderate intensity physical activity (brisk walking for 30 minutes on 4 days/week). We formed 30 walking groups under four coordinators, in a home-based setting with professional supervision and guidance. A log of physical activity sessions for the 10-week intervention period was maintained in the form of group attendance record. Village leaders, self-help groups and youth clubs were involved in promoting physical activity. Of the 485 subjects, 265 people (54.6%) engaged in brisk walking >4 days a week, while 156 subjects (32.2%) performed walking on 1-4 days per week during the intervention. The drop-out rate was 13.2% (64 subjects). Age, occupation and educational status were important determinants of participation and adherence to the physical activity programme. Application of social marketing techniques in an intervention to promote physical activity was successful in a rural Indian community. Studying the determinants of adoption of a physical activity programme and addressing the barriers to behaviour change are essential for designing relevant policies and effective programmes. Copyright 2012, NMJI.

  11. Long-term PAH monitoring results from the Anacostia River active capping demonstration using polydimethylsiloxane (PDMS) fibers.

    Science.gov (United States)

    Lampert, David J; Lu, Xiaoxia; Reible, Danny D

    2013-03-01

    In this paper, the long-term monitoring results for hydrophobic organic compounds, specifically polycyclic aromatic hydrocarbons (PAHs), from a field demonstration of capping contaminated sediments at the Anacostia River in Washington DC are presented and analyzed. In situ pore water concentrations in field-contaminated sediments in the demonstration caps were quantified using a polydimethylsiloxane (PDMS)-based passive sampling device. High resolution vertical pore water concentration profiles were measured using the device and were used to infer fate and transport of polycyclic aromatics hydrocarbons (PAHs) at the site. The derived pore water concentrations were compared with observed bioaccumulation and solid-phase concentration profiles to infer contaminant migration rates and mechanisms. Observed pore water concentrations were found to be a better predictor of bioaccumulation than solid-phase concentrations. Solid-phase concentrations were low in cores which implied containment of contamination; however pore water profiles showed that contaminant migration had occurred in the first few years after cap placement. The discrepancy is the result of the low sorption capacity of the sand. Because of surface re-contamination, low sorption capacity in the demonstration caps and strong tidal pumping effects, steady state contaminant profiles were reached in the caps several years after placement. Despite re-contamination at the surface, steady state concentrations in the capped areas showed decreased contamination levels relative to the control area.

  12. Observing Exoplanets with High-dispersion Coronagraphy. II. Demonstration of an Active Single-mode Fiber Injection Unit

    Energy Technology Data Exchange (ETDEWEB)

    Mawet, D.; Ruane, G.; Xuan, W.; Echeverri, D.; Klimovich, N.; Randolph, M.; Fucik, J.; Wang, J.; Dekany, R.; Delorme, J.-R. [Department of Astronomy, California Institute of Technology, 1200 East California Boulevard, MC 249-17, Pasadena, CA 91125 (United States); Wallace, J. K.; Vasisht, G.; Mennesson, B.; Choquet, E.; Serabyn, E., E-mail: dmawet@astro.caltech.edu [Jet Propulsion Laboratory, California Institute of Technology, 4800 Oak Grove Drive, Pasadena, CA 91109 (United States)

    2017-04-01

    High-dispersion coronagraphy (HDC) optimally combines high-contrast imaging techniques such as adaptive optics/wavefront control plus coronagraphy to high spectral resolution spectroscopy. HDC is a critical pathway toward fully characterizing exoplanet atmospheres across a broad range of masses from giant gaseous planets down to Earth-like planets. In addition to determining the molecular composition of exoplanet atmospheres, HDC also enables Doppler mapping of atmosphere inhomogeneities (temperature, clouds, wind), as well as precise measurements of exoplanet rotational velocities. Here, we demonstrate an innovative concept for injecting the directly imaged planet light into a single-mode fiber, linking a high-contrast adaptively corrected coronagraph to a high-resolution spectrograph (diffraction-limited or not). Our laboratory demonstration includes three key milestones: close-to-theoretical injection efficiency, accurate pointing and tracking, and on-fiber coherent modulation and speckle nulling of spurious starlight signal coupling into the fiber. Using the extreme modal selectivity of single-mode fibers, we also demonstrated speckle suppression gains that outperform conventional image-based speckle nulling by at least two orders of magnitude.

  13. Integrated Application of Active Controls (IAAC) technology to an advanced subsonic transpot project-demonstration act system definition

    Science.gov (United States)

    Hanks, G. W.; Shomber, H. A.; Crumb, C. B.; Flora, C. C.; Macdonald, K. A. B.; Smith, R. D.; Sassi, A. P.; Dorwart, R. J.

    1982-01-01

    The 1985 ACT airplane is the Final Active Controls Technology (ACT) Airplane with the addition of three-axis fly by wire. Thus it retains all the efficiency features of the full ACT system plus the weight and cost savings accruing from deletion of the mechanical control system. The control system implements the full IAAC spectrum of active controls except flutter-mode control, judged essentially nonbeneficial, and incorporates new control surfaces called flaperons to make the most of wing-load alleviation. This redundant electronic system is conservatively designed to preserve the extreme reliability required of crucial short-period pitch augmentation, which provides more than half of the fuel savings.

  14. Posttraumatic tibia valga: a case demonstrating asymmetric activity at the proximal growth plate on technetium bone scan

    International Nuclear Information System (INIS)

    Zionts, L.E.; Harcke, H.T.; Brooks, K.M.; MacEwen, G.D.

    1987-01-01

    Posttraumatic tibia valga is a well-recognized complication following fracture of the upper tibial metaphysis in young children. We present a case of a child who developed a valgus deformity following fracture of the proximal tibia and fibula in which quantitative bone scintigraphy at 5 months after injury demonstrated increased uptake at the proximal tibial growth plate with proportionally greater uptake on the medial side. This finding suggests that the valgus deformity in this patient was due to a relative increase in vascularity and consequent overgrowth of the medial portion of the proximal tibial physis

  15. Posttraumatic tibia valga: a case demonstrating asymmetric activity at the proximal growth plate on technetium bone scan

    Energy Technology Data Exchange (ETDEWEB)

    Zionts, L.E.; Harcke, H.T.; Brooks, K.M.; MacEwen, G.D.

    1987-07-01

    Posttraumatic tibia valga is a well-recognized complication following fracture of the upper tibial metaphysis in young children. We present a case of a child who developed a valgus deformity following fracture of the proximal tibia and fibula in which quantitative bone scintigraphy at 5 months after injury demonstrated increased uptake at the proximal tibial growth plate with proportionally greater uptake on the medial side. This finding suggests that the valgus deformity in this patient was due to a relative increase in vascularity and consequent overgrowth of the medial portion of the proximal tibial physis.

  16. Evaluating the performance of medical educators: a novel analysis tool to demonstrate the quality and impact of educational activities.

    Science.gov (United States)

    Chandran, Latha; Gusic, Maryellen; Baldwin, Constance; Turner, Teri; Zenni, Elisa; Lane, J Lindsey; Balmer, Dorene; Bar-On, Miriam; Rauch, Daniel A; Indyk, Diane; Gruppen, Larry D

    2009-01-01

    Traditional promotion standards rely heavily on quantification of research grants and publications in the curriculum vitae. The promotion and retention of educators is challenged by the lack of accepted standards to evaluate the depth, breadth, quality, and impact of educational activities. The authors sought to develop a practical analysis tool for the evaluation of educator portfolios (EPs), based on measurable outcomes that allow reproducible analysis of the quality and impact of educational activities. The authors, 10 veteran educators and an external expert evaluator, used a scholarly, iterative consensus-building process to develop the tool and test it using real EPs from educational scholars who followed an EP template. They revised the template in parallel with the analysis tool to ensure that EP data enabled valid and reliable evaluation. The authors created the EP template and analysis tool for scholar and program evaluation in the Educational Scholars Program, a three-year national certification program of the Academic Pediatric Association. The analysis tool combines 18 quantitative and 25 qualitative items, with specifications, for objective evaluation of educational activities and scholarship. The authors offer this comprehensive, yet practical tool as a method to enhance opportunities for faculty promotions and advancement, based on well-defined and documented educational outcome measures. It is relevant for clinical educators across disciplines and across institutions. Future studies will test the interrater reliability of the tool, using data from EPs written using the revised template.

  17. Peptide Inhibitor of Complement C1 (PIC1) demonstrates antioxidant activity via single electron transport (SET) and hydrogen atom transfer (HAT).

    Science.gov (United States)

    Gregory Rivera, Magdielis; Hair, Pamela S; Cunnion, Kenji M; Krishna, Neel K

    2018-01-01

    Reactive oxygen species (ROS) are natural byproducts of oxidative respiration that are toxic to organs and tissues. To mitigate ROS damage, organisms have evolved a variety of antioxidant systems to counteract these harmful molecules, however in certain pathological conditions these protective mechanisms can be overwhelmed. We have recently demonstrated that Peptide Inhibitor of Complement C1 (PIC1) mitigates peroxidase activity of the heme bearing proteins myeloperoxidase, hemoglobin, and myoglobin through a reversible process. To determine if this property of PIC1 was antioxidant in nature, we tested PIC1 in a number of well-established antioxidant assays. PIC1 showed dose-dependent antioxidant activity in a total antioxidant (TAC) assay, hydroxyl radical antioxidant capacity (HORAC) assay, oxygen radical antioxidant capacity (ORAC) assay as well as the thiobarbituric acid reactive substances (TBARS) assay to screen for PIC1 antioxidant activity in human plasma. The antioxidant activity of PIC1 in the TAC assay, as well as the HORAC/ORAC assay demonstrated that this peptide acts via the single electron transport (SET) and hydrogen atom transfer (HAT) mechanisms, respectively. Consistent with this mechanism of action, PIC1 did not show activity in a metal chelating activity (MCA) assay. PIC1 contains two vicinal cysteine residues and displayed similar antioxidant activity to the well characterized cysteine-containing tripeptide antioxidant molecule glutathione (GSH). Consistent with the role of the cysteine residues in the antioxidant activity of PIC1, oxidation of these residues significantly abrogated antioxidant activity. These results demonstrate that in addition to its described complement inhibiting activity, PIC1 displays in vitro antioxidant activity.

  18. Cortical activation in profoundly deaf patients during cochlear implant stimulation demonstrated by H sub 2 (15)O PET

    Energy Technology Data Exchange (ETDEWEB)

    Herzog, H.; Lamprecht, A.; Kuehn, A.R.; Roden, W.; Vosteen, K.H.; Feinendegen, L.E. (Institute of Medicine, Juelich, (West Germany))

    1991-05-01

    Cochlear implants (CIs) are used to provide sensations of sound to profoundly deaf patients. The performance of the CI is assessed mainly by the subjective reports of patients. The aim of this study was to look for objective cortical responses to the stimulation of the CI. Two postlingually and two prelingually deaf patients were investigated by positron emission tomography (PET) using {sup 15}O-labeled water (H{sub 2}{sup 15}O) to determine the regional cerebral blood flow (rCBF). Instead of quantifying rCBF in absolute terms, it was estimated by referring the regional tissue concentration of H{sub 2}{sup 15}O to the mean whole brain concentration. CI stimulation encoded from white noise and sequential words led to an increased rCBF in the primary and secondary (Wernicke) auditory cortex. Relative elevations of up to 33% were observed bilaterally, although they were higher contralateral to the CI. These results were obtained not only in the postlingually deaf patients but also in two patients who had never been able to hear. Thus, it could be demonstrated that PET measurements of cerebral H{sub 2}{sup 15}O distribution yield objective responses of the central auditory system during electrical stimulation by CIs in profoundly deaf patients.

  19. Solnatide Demonstrates Profound Therapeutic Activity in a Rat Model of Pulmonary Edema Induced by Acute Hypobaric Hypoxia and Exercise.

    Science.gov (United States)

    Zhou, Qiquan; Wang, Dong; Liu, Yunsheng; Yang, Xiaohong; Lucas, Rudolf; Fischer, Bernhard

    2017-03-01

    The synthetic peptide solnatide is a novel pharmacologic agent that reduces extravascular lung water, blunts reactive oxygen species production, and improves lung function due to its ability to directly activate the epithelial sodium channel. The goal of this study was to investigate the effect of solnatide in pulmonary edema induced by acute hypobaric hypoxia and exercise in rats, which is considered a model for high-altitude pulmonary edema. Sprague-Dawley rats were assigned to low-altitude control and eight treatment groups. Animals of all groups were subjected to exhaustive exercise in a hypobaric hypoxic environment simulating an altitude of 4,500 meters, followed by simulated ascent to 6,000 meters. After 48 h at 6,000 meters, rats were given sodium chloride, dexamethasone, aminophylline, p38 mitogen activated protein kinase inhibitor, and NOD-like receptor containing a pyrin domain 3 inhibitor, or one of three different doses of solnatide, once daily for 3 consecutive days. After 3 days, arterial blood gas, BAL fluid, lung water content, and histologic and ultra-microstructure analyses were performed. Tight junction protein occludin was assayed by using immunohistochemistry. Rats treated with solnatide had significantly lower BAL fluid protein and lung water content than high-altitude control rats. Lungs of solnatide-treated rats were intact and showed less hemorrhage and disruption of the alveolar-capillary barrier than those of high-altitude control animals. Occludin expression was significantly higher in solnatide-treated animals, compared with high-altitude control, dexamethasone-, and aminophylline-treated animals. Solnatide reduced pulmonary edema, increased occludin expression, and improved gas-blood barrier function during acute hypobaric hypoxia and exercise in rats. These results provide a rationale for the clinical application of solnatide to patients with pulmonary edema and exposure to a high-altitude hypoxic environment. Copyright © 2016 The

  20. Demonstration of a multiscale modeling technique: prediction of the stress–strain response of light activated shape memory polymers

    International Nuclear Information System (INIS)

    Beblo, Richard V; Weiland, Lisa Mauck

    2010-01-01

    Presented is a multiscale modeling method applied to light activated shape memory polymers (LASMPs). LASMPs are a new class of shape memory polymer (SMPs) being developed for adaptive structures applications where a thermal stimulus is undesirable. LASMP developmental emphasis is placed on optical manipulation of Young's modulus. A multiscale modeling approach is employed to anticipate the soft and hard state moduli solely on the basis of a proposed molecular formulation. Employing such a model shows promise for expediting down-selection of favorable formulations for synthesis and testing, and subsequently accelerating LASMP development. An empirical adaptation of the model is also presented which has applications in system design once a formulation has been identified. The approach employs rotational isomeric state theory to build a molecular scale model of the polymer chain yielding a list of distances between the predicted crosslink locations, or r-values. The r-values are then fitted with Johnson probability density functions and used with Boltzmann statistical mechanics to predict stress as a function of the strain of the phantom polymer network. Empirical adaptation for design adds junction constraint theory to the modeling process. Junction constraint theory includes the effects of neighboring chain interactions. Empirical fitting results in numerically accurate Young's modulus predictions. The system is modular in nature and thus lends itself well to being adapted to other polymer systems and development applications

  1. Leptin and Physical Activity in Adult Patients with Anorexia Nervosa: Failure to Demonstrate a Simple Linear Association

    Directory of Open Access Journals (Sweden)

    Andreas Stengel

    2017-11-01

    Full Text Available High physical activity (PA in patients with anorexia nervosa (AN is hypothesized to be, at least in part, a consequence of hypoleptinemia. However, most studies on the association of leptin and PA in AN were performed in adolescents or young adults, and PA was generally measured with subjective tools. We aimed to explore the association of leptin and PA in adults with AN using an objective technique to quantify PA. Using a cross-sectional, observational design, we analyzed body fat (bioelectrical impedance, PA (accelerometry, SenseWear™ armband and plasma leptin (ELISA in 61 women with AN (median age: 25 years, range: 18–52 years; median BMI: 14.8 ± 2.0 kg/m2 at the start of hospitalization. Results indicated a mean step count per day of 12,841 ± 6408 (range: 3956–37,750. Leptin was closely associated with BMI and body fat (ρ = 0.508 and ρ = 0.669, p < 0.001, but not with steps (ρ = 0.015, p = 0.908. Moreover, no significant association was observed between BMI and steps (ρ = 0.189, p = 0.146. In conclusion, there was no simple, linear association of leptin and PA, highlighting the need for more complex and non-linear models to analyze the association of leptin and PA in adults with AN in future studies.

  2. Preclinical demonstration of synergistic Active Nutrients/Drug (AND combination as a potential treatment for malignant pleural mesothelioma.

    Directory of Open Access Journals (Sweden)

    Viviana Volta

    Full Text Available Malignant pleural mesothelioma (MPM is a poor prognosis disease lacking adequate therapy. We have previously shown that ascorbic acid administration is toxic to MPM cells. Here we evaluated a new combined therapy consisting of ascorbate/epigallocatechin-3-gallate/gemcitabine mixture (called AND, for Active Nutrients/Drug. In vitro effects of AND therapy on various MPM cell lines revealed a synergistic cytotoxic mechanism. In vivo experiments on a xenograft mouse model for MPM, obtained by REN cells injection in immunocompromised mice, showed that AND strongly reduced the size of primary tumor as well as the number and size of metastases, and prevented abdominal hemorrhage. Kaplan Meier curves and the log-rank test indicated a marked increase in the survival of AND-treated animals. Histochemical analysis of dissected tumors showed that AND induced a shift from cell proliferation to apoptosis in cancer cells. Lysates of tumors from AND-treated mice, analyzed with an antibody array, revealed decreased TIMP-1 and -2 expressions and no effects on angiogenesis regulating factors. Multiplex analysis for signaling protein phosphorylation exhibited inactivation of cell proliferation pathways. The complex of data showed that the AND treatment is synergistic in vitro on MPM cells, and blocks in vivo tumor progression and metastasization in REN-based xenografts. Hence, the AND combination is proposed as a new treatment for MPM.

  3. Demonstration of 5'-nucleotidase activity in unfixed cryostat sections of rat liver using a combined light- and electron-microscope procedure

    NARCIS (Netherlands)

    Song, J.; Bosch, K. S.; Tigchelaar, W.; van den Munckhof, R. J.; Schellens, J. P.; van Noorden, C. J.; Frederiks, W. M.

    1995-01-01

    In the present study a technique was developed to demonstrate 5'-nucleotidase activity in unfixed cryostat sections of rat liver at the light- and electron-microscope level using a semipermeable membrane. In order to retain the ultrastructure of the unfixed material as much as possible, incubations

  4. Pyrene conjugation and spectroscopic analysis of hydroxypropyl methylcellulose compounds successfully demonstrated a local dielectric difference associated with in vivo anti-prion activity.

    Directory of Open Access Journals (Sweden)

    Kenta Teruya

    Full Text Available Our previous study on prion-infected rodents revealed that hydroxypropyl methylcellulose compounds (HPMCs with different molecular weights but similar composition and degree of substitution have different levels of long-lasting anti-prion activity. In this study, we searched these HPMCs for a parameter specifically associated with in vivo anti-prion activity by analyzing in vitro chemical properties and in vivo tissue distributions. Infrared spectroscopic and thermal analyses revealed no differences among HPMCs, whereas pyrene conjugation and spectroscopic analysis revealed that the fluorescence intensity ratio of peak III/peak I correlated with anti-prion activity. This correlation was more clearly demonstrated in the anti-prion activity of the 1-year pre-infection treatment than that of the immediate post-infection treatment. In addition, the intensity ratio of peak III/peak I negatively correlated with the macrophage uptake level of HPMCs in our previous study. However, the in vivo distribution pattern was apparently not associated with anti-prion activity and was different in the representative tissues. These findings suggest that pyrene conjugation and spectroscopic analysis are powerful methods to successfully demonstrate local dielectric differences in HPMCs and provide a feasible parameter denoting the long-lasting anti-prion activity of HPMCs in vivo.

  5. Experimental demonstration of the influence of alcohol advertising on the activation of alcohol expectancies in memory among fourth- and fifth-grade children.

    Science.gov (United States)

    Dunn, M E; Yniguez, R M

    1999-11-01

    Previous work has demonstrated that children's organization and activation of alcohol expectancies in memory vary as a function of alcohol use, even among children as young as in the 3rd grade. To advance the understanding of influences on the development of alcohol expectancies in children, 551 4th- and 5th-grade children were exposed to 5 beer commercials or 5 soft drink commercials. After viewing the advertisements, all children reported their 1st associate to an alcohol prompt and completed a memory model-based measure of children's alcohol expectancies. Multidimensional scaling was used to map expectancies into hypothetical memory network format, and preference mapping was used to derive possible paths of activation. Children who viewed beer commercials were more likely to activate positive and arousing alcohol expectancies. In view of previous findings demonstrating that this pattern of activation corresponded to higher drinking among 3rd, 6th, 9th, and 12th graders, the present findings suggested that antecedents to drinking like exposure to advertising may promote heavier drinking among children by influencing the activation of expectancies in memory.

  6. Raltegravir: first in class HIV integrase inhibitor

    Directory of Open Access Journals (Sweden)

    Zelalem Temesgen

    2008-06-01

    Full Text Available Zelalem Temesgen1, Dawd S Siraj21Mayo Clinic, Rochester, MN, USA; 2East Carolina University Greenville, NC, USAAbstract: On October 16, 2007, the US Food and Drug Administration (FDA approved raltegravir for treatment of human immunodeficiency virus (HIV-1 infection in combination with other antiretroviral agents in treatment-experienced adult patients who have evidence of viral replication and HIV-1 strains resistant to multiple antiretroviral agents. Raltegravir is first in a novel class of antiretroviral drugs known as integrase inhibitors. It has demonstrated potent anti HIV activity in both antiretroviral treatment-naïve and experienced patients. The most common adverse events reported with raltegravir during phase 2 and 3 clinical trials were diarrhea, nausea, and headache. Laboratory abnormalities include mild elevations in liver transaminases and creatine phosphokinase.Keywords: raltegravir, HIV, antiretroviral agents, integrase inhibitors

  7. Active Flow Control (AFC) and Insect Accretion and Mitigation (IAM) System Design and Integration on the Boeing 757 ecoDemonstrator

    Science.gov (United States)

    Alexander, Michael G.; Harris, F. Keith; Spoor, Marc A.; Boyland, Susannah R.; Farrell, Thomas E.; Raines, David M.

    2016-01-01

    This paper presents a systems overview of how the Boeing and NASA team designed, analyzed, fabricated, and integrated the Active Flow Control (AFC) technology and Insect Accretion Mitigation (IAM) systems on the Boeing 757 ecoDemonstrator. The NASA Environmentally Responsible Aviation (ERA) project partnered with Boeing to demonstrate these two technology systems on a specially outfitted Boeing 757 ecoDemonstrator during the spring of 2015. The AFC system demonstrated attenuation of flow separation on a highly deflected rudder and increased the side force generated. This AFC system may enable a smaller vertical tail to provide the control authority needed in the event of an engine failure during takeoff while still operating in a conventional manner over the rest of the flight envelope. The AFC system consisted of ducting to obtain air from the Auxiliary Power Unit (APU), a control valve to modulate the system mass flow, a heat exchanger to lower the APU air temperature, and additional ducting to deliver the air to the AFC actuators located on the vertical tail. The IAM system demonstrated how to mitigate insect residue adhesion on a wing's leading edge. Something as small as insect residue on a leading edge can cause turbulent wedges that interrupt laminar flow, resulting in an increase in drag and fuel use. The IAM system consisted of NASA developed Engineered Surfaces (ES) which were thin aluminum sheet substrate panels with coatings applied to the exterior. These ES were installed on slats 8 and 9 on the right wing of the 757 ecoDemonstrator. They were designed to support panel removal and installation in one crew shift. Each slat accommodated 4 panels. Both the AFC and IAM flight test were the culmination of several years of development and produced valuable data for the advancement of modern aircraft designs.

  8. Simultaneous production of l-lactic acid with high optical activity and a soil amendment with food waste that demonstrates plant growth promoting activity.

    Science.gov (United States)

    Kitpreechavanich, Vichien; Hayami, Arisa; Talek, Anfal; Chin, Clament Fui Seung; Tashiro, Yukihiro; Sakai, Kenji

    2016-07-01

    A unique method to produce highly optically-active l-lactic acid and soil amendments that promote plant growth from food waste was proposed. Three Bacillus strains Bacillus subtilis KBKU21, B. subtilis N3-9 and Bacillus coagulans T27, were used. Strain KBKU21 accumulated 36.9 g/L l-lactic acid with 95.7% optical activity and 98.2% l-lactic acid selectivity when fermented at 43°C for 84 h in a model kitchen refuse (MKR) medium. Residual precipitate fraction (anaerobically-fermented MKR (AFM) compost) analysis revealed 4.60%, 0.70% and 0.75% of nitrogen (as N), phosphorous (as P2O5), and potassium (as K2O), respectively. Additionally, the carbon to nitrogen ratio decreased from 13.3 to 10.6. AFM compost with KBKU21 promoted plant growth parameters, including leaf length, plant height and fresh weight of Brassica rapa (Komatsuna), than that by chemical fertilizers or commercial compost. The concept provides an incentive for the complete recycling of food waste, contributing towards a sustainable production system. Copyright © 2016 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.

  9. The C-terminal region of the non-structural protein 2B from Hepatitis A Virus demonstrates lipid-specific viroporin-like activity

    Science.gov (United States)

    Shukla, Ashutosh; Dey, Debajit; Banerjee, Kamalika; Nain, Anshu; Banerjee, Manidipa

    2015-10-01

    Viroporins are virally encoded, membrane-active proteins, which enhance viral replication and assist in egress of viruses from host cells. The 2B proteins in the picornaviridae family are known to have viroporin-like properties, and play critical roles during virus replication. The 2B protein of Hepatitis A Virus (2B), an unusual picornavirus, is somewhat dissimilar from its analogues in several respects. HAV 2B is approximately 2.5 times the length of other 2B proteins, and does not disrupt calcium homeostasis or glycoprotein trafficking. Additionally, its membrane penetrating properties are not yet clearly established. Here we show that the membrane interacting activity of HAV 2B is localized in its C-terminal region, which contains an alpha-helical hairpin motif. We show that this region is capable of forming small pores in membranes and demonstrates lipid specific activity, which partially rationalizes the intracellular localization of full-length 2B. Using a combination of biochemical assays and molecular dynamics simulation studies, we also show that HAV 2B demonstrates a marked propensity to dimerize in a crowded environment, and probably interacts with membranes in a multimeric form, a hallmark of other picornavirus viroporins. In sum, our study clearly establishes HAV 2B as a bona fide viroporin in the picornaviridae family.

  10. Demonstration of extracellular peptidylarginine deiminase (PAD) activity in synovial fluid of patients with rheumatoid arthritis using a novel assay for citrullination of fibrinogen

    DEFF Research Database (Denmark)

    Damgaard, Dres; Senolt, Ladislav; Nielsen, Michael Friberg

    2014-01-01

    in general. PAD activity has been demonstrated in various cell lysates, but so far not in synovial fluid. We aimed to develop an assay for detection of PAD activity, if any, in synovial fluid from RA patients. METHODS: An enzyme-linked immunosorbent assay using human fibrinogen as the immobilized substrate...... for citrullination and anti-citrullinated fibrinogen antibody as the detecting agent were used for measurement of PAD activity in synovial fluid samples from five RA patients. The concentrations of PAD2 and calcium were also determined. RESULTS: Approximately 150 times lower levels of recombinant human PAD2 (rhPAD2......) than of rhPAD4 were required for citrullination of fibrinogen. PAD activity was detected in four of five synovial fluid samples from RA patients and correlated with PAD2 concentrations in the samples (r = 0.98, P = 0.003). The calcium requirement for half-maximal activities of PAD2 and PAD4 were found...

  11. Inhibitors of bacterial N-succinyl-L,L-diaminopimelic acid desuccinylase (DapE) and demonstration of in vitro antimicrobial activity.

    Science.gov (United States)

    Gillner, Danuta; Armoush, Nicola; Holz, Richard C; Becker, Daniel P

    2009-11-15

    The dapE-encoded N-succinyl-L,L-diaminopimelic acid desuccinylase (DapE) is a critical bacterial enzyme for the construction of the bacterial cell wall. A screen biased toward compounds containing zinc-binding groups (ZBG's) including thiols, carboxylic acids, boronic acids, phosphonates and hydroxamates has delivered a number of micromolar inhibitors of DapE from Haemophilus influenzae, including the low micromolar inhibitor L-captopril (IC(50)=3.3 microM, K(i)=1.8 microM). In vitro antimicrobial activity was demonstrated for L-captopril against Escherichia coli.

  12. LIMB Demonstration Project Extension and Coolside Demonstration

    Energy Technology Data Exchange (ETDEWEB)

    Goots, T.R.; DePero, M.J.; Nolan, P.S.

    1992-11-10

    This report presents results from the limestone Injection Multistage Burner (LIMB) Demonstration Project Extension. LIMB is a furnace sorbent injection technology designed for the reduction of sulfur dioxide (SO[sub 2]) and nitrogen oxides (NO[sub x]) emissions from coal-fired utility boilers. The testing was conducted on the 105 Mwe, coal-fired, Unit 4 boiler at Ohio Edison's Edgewater Station in Lorain, Ohio. In addition to the LIMB Extension activities, the overall project included demonstration of the Coolside process for S0[sub 2] removal for which a separate report has been issued. The primary purpose of the DOE LIMB Extension testing, was to demonstrate the generic applicability of LIMB technology. The program sought to characterize the S0[sub 2] emissions that result when various calcium-based sorbents are injected into the furnace, while burning coals having sulfur content ranging from 1.6 to 3.8 weight percent. The four sorbents used included calcitic limestone, dolomitic hydrated lime, calcitic hydrated lime, and calcitic hydrated lime with a small amount of added calcium lignosulfonate. The results include those obtained for the various coal/sorbent combinations and the effects of the LIMB process on boiler and plant operations.

  13. Implementation of the k{sub 0}-standardization Method for an Instrumental Neutron Activation Analysis: Use-k{sub 0}-IAEA Software as a Demonstration

    Energy Technology Data Exchange (ETDEWEB)

    Chung, Yong Sam; Moon, Jong Hwa; Kim, Sun Ha; Kim, Hark Rho [Korea Atomic Energy Research Institute, Taejon (Korea, Republic of); Ho, Manh Dung [Nuclear Research Institute, Dalat (Viet Nam)

    2006-03-15

    Under the RCA post-doctoral program, from May 2005 through February 2006, it was an opportunity to review the present work being carried out in the Neutron Activation Analysis Laboratory, HANARO Center, KAERI. The scope of this research included: a calibration of the counting system, a characterization of the irradiation facility ,a validation of the established k{sub o}-NAA procedure.The k{sub o}-standardization method for an Neutron Activation Analysis(k{sub o}-NAA), which is becoming increasingly popular and widespread,is an absolute calibration technique where the nuclear data are replaced by compound nuclear constants which are experimentally determined. The k{sub o}-IAEA software distributed by the IAEA in 2005 was used as a demonstration for this work. The NAA no. 3 irradiation hole in the HANARO research reactor and the gamma-ray spectrometers No. 1 and 5 in the NAA Laboratory were used.

  14. Implementation of the k0-standardization Method for an Instrumental Neutron Activation Analysis: Use-k0-IAEA Software as a Demonstration

    International Nuclear Information System (INIS)

    Chung, Yong Sam; Moon, Jong Hwa; Kim, Sun Ha; Kim, Hark Rho; Ho, Manh Dung

    2006-03-01

    Under the RCA post-doctoral program, from May 2005 through February 2006, it was an opportunity to review the present work being carried out in the Neutron Activation Analysis Laboratory, HANARO Center, KAERI. The scope of this research included: a calibration of the counting system, a characterization of the irradiation facility ,a validation of the established k o -NAA procedure.The k o -standardization method for an Neutron Activation Analysis(k o -NAA), which is becoming increasingly popular and widespread,is an absolute calibration technique where the nuclear data are replaced by compound nuclear constants which are experimentally determined. The k o -IAEA software distributed by the IAEA in 2005 was used as a demonstration for this work. The NAA no. 3 irradiation hole in the HANARO research reactor and the gamma-ray spectrometers No. 1 and 5 in the NAA Laboratory were used

  15. Assisting children with attention deficit hyperactivity disorder to reduce the hyperactive behavior of arbitrary standing in class with a Nintendo Wii remote controller through an active reminder and preferred reward stimulation.

    Science.gov (United States)

    Shih, Ching-Hsiang; Wang, Shu-Hui; Wang, Yun-Ting

    2014-09-01

    Recent studies in the field of special education have shown that in combination with software technology, high-tech commercial products can be applied as useful assistive technology devices to help people with disabilities. This study extended this concept to turn a Nintendo Wii Remote Controller into a high-performance limb action detector, in order to evaluate whether two students with Attention Deficit Hyperactivity Disorder (ADHD) could reduce their hyperactive behavior through an active reminder and stimulation in the form of the participants' preferred rewards. This study focused on one particular hyperactive behavior common to both students: standing up arbitrarily during class. The active reminder was in the form of vibration feedback provided via the built-in function of the Wii Remote Controller, which was controlled and triggered by a control system to remind participants when they were engaging in standing behavior. This study was performed according to a multiple baseline design across participants. The results showed that both participants significantly improved their control over their hyperactive behavior during the intervention phase, and retained this effective performance in the maintenance phase. The practical and developmental implications of the findings are discussed. Copyright © 2014 Elsevier Ltd. All rights reserved.

  16. Palpability Support Demonstrated

    DEFF Research Database (Denmark)

    Brønsted, Jeppe Rørbæk; Grönvall, Erik; Fors, David

    2007-01-01

    In ubiquitous computing, as more and more devices are embedded into the environment, there is a risk that the user loses the understanding of the system. In normal use this is not always a problem, but when breakdowns occur it is crucial that the user understands the system to be able to handle...... is based on the Active Surfaces concept in which therapists rehabilitate physically and mentally impaired children by means of an activity that stimulates the children both physically and cognitively. In this paper we demonstrate how palpability can be supported in a prototype of the Active Surfaces...

  17. Palpability Support Demonstrated

    DEFF Research Database (Denmark)

    Brønsted, Jeppe; Grönvall, Erik; Fors, David

    2007-01-01

    the situation. The concept of palpable computing, introduced by the PalCom project, denotes systems which support such understandability. In PalCom, a set of prototype scenarios provide input for an open software architecture and a conceptual framework for palpable computing. One of these prototype scenarios...... is based on the Active Surfaces concept in which therapists rehabilitate physically and mentally impaired children by means of an activity that stimulates the children both physically and cognitively. In this paper we demonstrate how palpability can be supported in a prototype of the Active Surfaces...

  18. Innovative technology demonstration

    International Nuclear Information System (INIS)

    Anderson, D.B.; Luttrell, S.P.; Hartley, J.N.; Hinchee, R.

    1992-04-01

    The Innovative Technology Demonstration (ITD) program at Tinker Air Force Base (TAFB), Oklahoma City, Oklahoma, will demonstrate the overall utility and effectiveness of innovative technologies for site characterization, monitoring, and remediation of selected contaminated test sites. The current demonstration test sites include a CERCLA site on the NPL list, located under a building (Building 3001) that houses a large active industrial complex used for rebuilding military aircraft, and a site beneath and surrounding an abandoned underground tank vault used for storage of jet fuels and solvents. The site under Building 3001 (the NW Test Site) is contaminated with TCE and Cr +6 ; the site with the fuel storage vault (the SW Tanks Site) is contaminated with fuels, BTEX and TCE. These sites and others have been identified for cleanup under the Air Force's Installation Restoration Program (IRP). This document describes the demonstrations that have been conducted or are planned for the TAFB

  19. In-Class Robot Flyby of an Endoplanet

    Science.gov (United States)

    Chadwick, A. J.; Capaldi, T.; Aurnou, J. M.

    2013-12-01

    For our Introduction to Computing class, we have developed a miniature robotic spacecraft mission that performs a flyby of an in-class 'endoplanet.' Our constructed endoplanet contains an internal dipole magnet, tilted with a dip angle that is unknown a priori. The spacecraft analog is a remotely controlled LEGO MINDSTORMS robot programmed using LabVIEW. Students acquire magnetic field data via a first spacecraft flyby past the endoplanet. This dataset is then imported into MATLAB, and is inverted to create a model of the magnet's orientation and dipole moment. Students use their models to predict the magnetic field profile along a different flyby path. They then test the accuracy of their models, comparing their predictions against the data acquired from this secondary flyby. We will be demonstrating this device at our poster in the Moscone Center.

  20. Factors Related to In-Class Spiritual Experience: Relationship between Pre-Class Scripture Reading, In-Class Note-Taking, and Perceived In-Class Spiritual Experiences

    Science.gov (United States)

    Hilton, John, III; Sweat, Anthony R.; Plummer, Kenneth

    2015-01-01

    The purpose of this study is to examine the relationship between student in-class note-taking and pre-class reading with perceived in-class spiritual and religious outcomes. This study surveyed 620 students enrolled in six different sections of an introductory religion course at a private religious university. Full-time religious faculty members…

  1. Buried Waste Integrated Demonstration

    International Nuclear Information System (INIS)

    1994-03-01

    The Buried Waste Integrated Demonstration (BWID) supports the applied research, development, demonstration, and evaluation of a suite of advanced technologies that offer promising solutions to the problems associated with the remediation of buried waste. BWID addresses the difficult remediation problems associated with DOE complex-wide buried waste, particularly transuranic (TRU) contaminated buried waste. BWID has implemented a systems approach to the development and demonstration of technologies that will characterize, retrieve, treat, and dispose of DOE buried wastes. This approach encompasses the entire remediation process from characterization to post-monitoring. The development and demonstration of the technology is predicated on how a technology fits into the total remediation process. To address all of these technological issues, BWID has enlisted scientific expertise of individuals and groups from within the DOE Complex, as well as experts from universities and private industry. The BWID mission is to support development and demonstration of a suite of technologies that, when integrated with commercially-available technologies, forms a comprehensive, remediation system for the effective and efficient remediation of buried waste throughout the DOE Complex. BWID will evaluate and validate demonstrated technologies and transfer this information and equipment to private industry to support the Office of Environmental Restoration (ER), Office of Waste Management (WM), and Office of Facility Transition (FT) remediation planning and implementation activities

  2. Structural and functional analysis of the Rous Sarcoma virus negative regulator of splicing and demonstration of its activation by the 9G8 SR protein.

    Science.gov (United States)

    Bar, Aileen; Marchand, Virginie; Khoury, Georges; Dreumont, Natacha; Mougin, Annie; Robas, Nathalie; Stévenin, James; Visvikis, Athanase; Branlant, Christiane

    2011-04-01

    Retroviruses require both spliced and unspliced RNAs for replication. Accumulation of Rous Sarcoma virus (RSV) unspliced RNA depends upon the negative regulator of splicing (NRS). Its 5'-part is considered as an ESE binding SR proteins. Its 3'-part contains a decoy 5'-splice site (ss), which inhibits splicing at the bona fide 5'-ss. Only the 3D structure of a small NRS fragment had been experimentally studied. Here, by chemical and enzymatic probing, we determine the 2D structure of the entire RSV NRS. Structural analysis of other avian NRSs and comparison with all sequenced avian NRSs is in favour of a phylogenetic conservation of the NRS 2D structure. By combination of approaches: (i) in vitro and in cellulo splicing assays, (ii) footprinting assays and (iii) purification and analysis of reconstituted RNP complex, we define a small NRS element retaining splicing inhibitory property. We also demonstrate the capability of the SR protein 9G8 to increase NRS activity in vitro and in cellulo. Altogether these data bring new insights on how NRS fine tune splicing activity.

  3. Ceftaroline plus Avibactam Demonstrates Bactericidal Activity against Pathogenic Anaerobic Bacteria in a One-Compartment In Vitro Pharmacokinetic/Pharmacodynamic Model

    Science.gov (United States)

    Werth, Brian J.

    2014-01-01

    Anaerobic pathogens are often associated with polymicrobial infections, such as diabetic foot infections. Patients with these infections are often treated with broad-spectrum, multidrug therapies targeting resistant Gram-positive bacteria, such as methicillin-resistant Staphylococcus aureus, as well as Gram-negative bacteria and anaerobes. The broad-spectrum, non-beta-lactam, beta-lactamase inhibitor avibactam has been combined with ceftaroline and may provide a single-product alternative for complicated polymicrobial infections. We compared the activity of ceftaroline-avibactam (CPA) to that of ertapenem (ERT) against common anaerobic pathogens in an in vitro pharmacokinetic/pharmacodynamic (PK/PD) model. Simulations of doses of ceftaroline-fosamil at 600 mg every 8 h (q8h) (maximum free drug concentration [fCmax], 17.04 mg/liter, and half-life [t1/2], 2.66 h) plus avibactam at 600 mg q8h (fCmax, 11.72 mg/liter, and t1/2, 1.8 h) and of ertapenem at 1 g q24h (fCmax, 13 mg/liter, and t1/2, 4 h) were evaluated against two strains of Bacteroides fragilis, one strain of Prevotella bivia, and one strain of Finegoldia magna in an anaerobic one-compartment in vitro PK/PD model over 72 h with a starting inoculum of ∼8 log10 CFU/ml. Bactericidal activity was defined as a reduction of ≥3 log10 CFU/ml from the starting inoculum. Both CPA and ERT were bactericidal against all four strains. CPA demonstrated improved activity against Bacteroides strains compared to that of ERT but had similar activity against Finegoldia magna and P. bivia, although modest regrowth was observed with CPA against P. bivia. No resistance emerged from any of the models. The pharmacokinetics achieved were 92 to 105% of the targets. CPA has potent in vitro activity against common anaerobic pathogens at clinically relevant drug exposures and may be a suitable single product for the management of complicated polymicrobial infections. PMID:24217692

  4. Can Facebook pages be a mode of blended learning to supplement in-class teaching in Saudi Arabia?

    Science.gov (United States)

    Anwar, Khurshid; Sajid, Muhammad Raihan; Cahusac, Peter; Shaikh, Abdul Ahad; Elgammal, Ahmad; Alshedoukhy, Ahlam; Kashir, Junaid

    2017-09-01

    The aim of this study was to examine the potential of a self-designed Facebook page on Neuroscience, to supplement in-class teaching as a mode of blended learning. Posts were split into multiple choice questions (MCQs), general interest articles, neuroscience-related external links and resources, and lecture notes and PowerPoint presentations. The study was divided into three distinct phases: before, during, and after the Neuroscience block. Student responses were evaluated via a self-developed questionnaire. Grades achieved by students undertaking the block in 2015 and 2014 were recorded, as were the grades achieved by the same cohort in concurrent blocks in the same year of study. Results showed that ~80% of students reported that use of the page enhanced their overall subject knowledge and exam preparation. Highest page activity occurred during the Neuroscience block. Peak activity occurred directly before summative assessments, with MCQ posts having the highest impact. The cohort of students with access to the Facebook page achieved better grades in the block compared with the previous cohort, despite similar average performance in other subjects. We demonstrate the utility of Facebook as a powerful tool for undergraduate education, supplementing in-class teaching, and assisting in exam preparation, potentially increasing average student performance. Copyright © 2017 the American Physiological Society.

  5. Innovative technology demonstrations

    International Nuclear Information System (INIS)

    Anderson, D.B.; Hartley, J.N.; Luttrell, S.P.

    1992-04-01

    Currently, several innovative technologies are being demonstrated at Tinker Air Force Base (TAFB) to address specific problems associated with remediating two contaminated test sites at the base. Cone penetrometer testing (CPT) is a form of testing that can rapidly characterize a site. This technology was selected to evaluate its applicability in the tight clay soils and consolidated sandstone sediments found at TAFB. Directionally drilled horizontal wells have been successfully installed at the US Department of Energy's (DOE) Savannah River Site to test new methods of in situ remediation of soils and ground water. This emerging technology was selected as a method that may be effective in accessing contamination beneath Building 3001 without disrupting the mission of the building, and in enhancing the extraction of contamination both in ground water and in soil. A soil gas extraction (SGE) demonstration, also known as soil vapor extraction, will evaluate the effectiveness of SGE in remediating fuels and TCE contamination contained in the tight clay soil formations surrounding the abandoned underground fuel storage vault located at the SW Tanks Site. In situ sensors have recently received much acclaim as a technology that can be effective in remediating hazardous waste sites. Sensors can be useful for determining real-time, in situ contaminant concentrations during the remediation process for performance monitoring and in providing feedback for controlling the remediation process. A demonstration of two in situ sensor systems capable of providing real-time data on contamination levels will be conducted and evaluated concurrently with the SGE demonstration activities. Following the SGE demonstration, the SGE system and SW Tanks test site will be modified to demonstrate bioremediation as an effective means of degrading the remaining contaminants in situ

  6. The novel deacetylase inhibitor AR-42 demonstrates pre-clinical activity in B-cell malignancies in vitro and in vivo.

    Directory of Open Access Journals (Sweden)

    David M Lucas

    2010-06-01

    Full Text Available While deacetylase (DAC inhibitors show promise for the treatment of B-cell malignancies, those introduced to date are weak inhibitors of class I and II DACs or potent inhibitors of class I DAC only, and have shown suboptimal activity or unacceptable toxicities. We therefore investigated the novel DAC inhibitor AR-42 to determine its efficacy in B-cell malignancies.In mantle cell lymphoma (JeKo-1, Burkitt's lymphoma (Raji, and acute lymphoblastic leukemia (697 cell lines, the 48-hr IC(50 (50% growth inhibitory concentration of AR-42 is 0.61 microM or less. In chronic lymphocytic leukemia (CLL patient cells, the 48-hr LC(50 (concentration lethal to 50% of AR-42 is 0.76 microM. AR-42 produces dose- and time-dependent acetylation both of histones and tubulin, and induces caspase-dependent apoptosis that is not reduced in the presence of stromal cells. AR-42 also sensitizes CLL cells to TNF-Related Apoptosis Inducing Ligand (TRAIL, potentially through reduction of c-FLIP. AR-42 significantly reduced leukocyte counts and/or prolonged survival in three separate mouse models of B-cell malignancy without evidence of toxicity.Together, these data demonstrate that AR-42 has in vitro and in vivo efficacy at tolerable doses. These results strongly support upcoming phase I testing of AR-42 in B-cell malignancies.

  7. Functional impairments in patients with borderline personality disorders demonstrated by neurospect HMPAO Tc99 in basal conditions and under frontal activation

    International Nuclear Information System (INIS)

    Prado, Cristian; Mena G, Ismael; Correa, Maria del Pilar

    2000-01-01

    We study a sample of 18 patients in basal conditions and 31 patients with diagnosis of Borderline Personality Disorder (DSM-IV Criteria) during cortical activation by means of the Wisconsin card sorting test and assessing function/cerebral blood flow by means of HMPAO Tc 99m NeuroSPECT. The results of changes of cerebral blood flow are shown statistically in a parametric image expressing standard deviations above or below the means of a normative data base for the corresponding age of the patient. We consider only as having significance levels below 2 standard deviations of the normal means . Over this parametric map we project a matrix of Brodmann areas developed by our group in order to precisely localize the areas of abnormality observed. We express our results as percentages of the areas of Brodmann that demonstrates hypoperfusion and we compare the results in a population studied in basal conditions (n=18) and (n=31) during activation by means of the Wisconsin card sorting test. In our results we highlight, in order of importance areas of paradoxical hypoperfusion in conditions of activation versus basal measurements in anterior cyngulate gyrus (Area 24) in both hemispheres. This is followed in importance by Subgenual area (Area 25), area 40 and area 32 in the left hemisphere, and area 28 in the right hemisphere, then followed by area 28 and area 36 in the left hemisphere, area M* and area 44 in both hemispheres, and areas 32, 9 and 46 of Brodmann in the right hemisphere. We concluded that there is a dysfunctional correlation of frontal function in borderline personality disorder. Particularly noticeable is the lack of motivation when there are changes in plans and conduct, lack of pleasure and loss of the meaning of a task during the cortical stimulation. In particular, the Brodmann areas 24, 25 and 32, linked to motivation show a wider involvement when they are exposed to changes in planning and in coping strategies, as it happens during the Wisconsin Test

  8. Commercial incineration demonstration

    International Nuclear Information System (INIS)

    Vavruska, J.S.; Borduin, L.C.

    1982-01-01

    Low-level radioactive wastes (LLW) generated by nuclear utilities presently are shipped to commercial burial grounds for disposal. Increasing transportation and disposal costs have caused industry to consider incineration as a cost-effective means of volume reduction of combustible LLW. Repeated inquiries from the nuclear industry regarding the applicability of the Los Alamos controlled air incineration (CAI) design led the DOE to initiate a commercial demonstration program in FY-1980. Development studies and results in support of this program involving ion exchange resin incineration and fission/activation product distributions within the Los Alamos CAI are described

  9. Visual Electricity Demonstrator

    Science.gov (United States)

    Lincoln, James

    2017-09-01

    The Visual Electricity Demonstrator (VED) is a linear diode array that serves as a dynamic alternative to an ammeter. A string of 48 red light-emitting diodes (LEDs) blink one after another to create the illusion of a moving current. Having the current represented visually builds an intuitive and qualitative understanding about what is happening in a circuit. In this article, I describe several activities for this device and explain how using this technology in the classroom can enhance the understanding and appreciation of physics.

  10. Final Demonstrator Status

    DEFF Research Database (Denmark)

    Workspace

    2003-01-01

    understanding the spatial relations that obtain between the landscape architects and their materials, their work settings and their work sites.  This report should be read as an introduction and orientation to the various project  prototypes and demonstrators – it will make only limited sense taken in isolation.  ......The WorkSPACE project aims to create an integrated environment for collaborative  work activities that can be distributed over a variety of spaces and that mixes digital  and physical materials. We have termed this ‘spatial computing’ because much of the  functionality is provided through...

  11. Gait and Function in Class III Obesity

    Directory of Open Access Journals (Sweden)

    Catherine Ling

    2012-01-01

    Full Text Available Walking, more specifically gait, is an essential component of daily living. Walking is a very different activity for individuals with a Body Mass Index (BMI of 40 or more (Class III obesity compared with those who are overweight or obese with a BMI between 26–35. Yet all obesity weight classes receive the same physical activity guidelines and recommendations. This observational study examined the components of function and disability in a group with Class III obesity and a group that is overweight or has Class I obesity. Significant differences were found between the groups in the areas of gait, body size, health condition, and activity capacity and participation. The Timed Up and Go test, gait velocity, hip circumference, and stance width appear to be most predictive of activity capacity as observed during gait assessment. The findings indicate that Class III-related gait is pathologic and not a normal adaptation.

  12. Spent fuel pyroprocessing demonstration

    International Nuclear Information System (INIS)

    McFarlane, L.F.; Lineberry, M.J.

    1995-01-01

    A major element of the shutdown of the US liquid metal reactor development program is managing the sodium-bonded spent metallic fuel from the Experimental Breeder Reactor-II to meet US environmental laws. Argonne National Laboratory has refurbished and equipped an existing hot cell facility for treating the spent fuel by a high-temperature electrochemical process commonly called pyroprocessing. Four products will be produced for storage and disposal. Two high-level waste forms will be produced and qualified for disposal of the fission and activation products. Uranium and transuranium alloys will be produced for storage pending a decision by the US Department of Energy on the fate of its plutonium and enriched uranium. Together these activities will demonstrate a unique electrochemical treatment technology for spent nuclear fuel. This technology potentially has significant economic and technical advantages over either conventional reprocessing or direct disposal as a high-level waste option

  13. Studying the effectiveness of activated carbon R95 respirators in reducing the inhalation of combustion by-products in Hanoi, Vietnam: a demonstration study

    Directory of Open Access Journals (Sweden)

    Wertheim Heiman FL

    2012-09-01

    Full Text Available Abstract Background Urban air pollution is an increasing health problem, particularly in Asia, where the combustion of fossil fuels has increased rapidly as a result of industrialization and socio-economic development. The adverse health impacts of urban air pollution are well established, but less is known about effective intervention strategies. In this demonstration study we set out to establish methods to assess whether wearing an R95 activated carbon respirator could reduce intake of polycyclic aromatic hydrocarbons (PAH in street workers in Hanoi, Vietnam. Methods In this demonstration study we performed a cross-over study in which non-smoking participants that worked at least 4 hours per day on the street in Hanoi were randomly allocated to specific respirator wearing sequences for a duration of 2 weeks. Urines were collected after each period, i.e. twice per week, at the end of the working day to measure hydroxy PAHs (OH-PAH using gas chromatography/high resolution mass spectrometry. The primary endpoint was the urinary concentration of 1-hydroxypyrene (1-OHP. Results Forty-four participants (54.5% male, median age 40 years were enrolled with the majority being motorbike taxi drivers (38.6% or street vendors (34.1%. The baseline creatinine corrected urinary level for 1-OHP was much higher than other international comparisons: 1020 ng/g creatinine (IQR: 604–1551. Wearing a R95 mask had no significant effect on 1-OHP levels: estimated multiplicative effect 1.0 (95% CI: 0.92-1.09 or other OH-PAHs, except 1-hydroxynaphthalene (1-OHN: 0.86 (95% CI: 0.11-0.96. Conclusions High levels of urine OH-PAHs were found in Hanoi street workers. No effect was seen on urine OH-PAH levels by wearing R95 particulate respirators in an area of high urban air pollution, except for 1-OHN. A lack of effect may be de to gaseous phase PAHs that were not filtered efficiently by the respirator. The high levels of urinary OH-PAHs found, urges for effective

  14. Demonstration of natriuretic activity in urine of neurosurgical patients with renal salt wasting [v2; ref status: indexed, http://f1000r.es/1ax

    Directory of Open Access Journals (Sweden)

    Steven J Youmans

    2013-06-01

    Full Text Available We have utilized the persistent elevation of fractional excretion (FE of urate, > 10%, to differentiate cerebral/renal salt wasting (RSW from the syndrome of inappropriate antidiuretic hormone secretion (SIADH, in which a normalization of FEurate occurs after correction of hyponatremia.  Previous studies suggest as well  that an elevated FEurate with normonatremia, without pre-existing hyponatremia, is also consistent with RSW, including studies demonstrating induction of RSW in rats infused with plasma from normonatremic neurosurgical and Alzheimer’s disease patients.  The present studies were designed to test whether precipitates from the urine of normonatremic neurosurgical patients, with either normal or elevated FEurate, and patients with SIADH, display natriuretic activity.   Methods: Ammonium sulfate precipitates from the urine of 6 RSW and 5 non-RSW Control patients were dialyzed (10 kDa cutoff to remove the ammonium sulfate, lyophilized, and the reconstituted precipitate was tested for its effect on transcellular transport of 22Na across LLC-PK1 cells grown to confluency in transwells. Results: Precipitates from 5 of the 6 patients with elevated FEurate and normonatremia significantly inhibited the in vitro transcellular transport of 22Na above a concentration of 3 μg protein/ml, by 10-25%, versus to vehicle alone, and by 15-40% at concentrations of 5-20 μg/ml as compared to precipitates from 4 of the 5 non-RSW patients with either normal FEurate and normonatremia (2 patients or with SIADH (2 patients. Conclusion: These studies provide further evidence that an elevated FEurate with normonatremia is highly consistent with RSW.  Evidence in the urine of natriuretic activity suggests significant renal excretion of the natriuretic factor. The potentially large source of the natriuretic factor that this could afford, coupled with small analytical sample sizes required by the in-vitro bioassay used here, should facilitate future

  15. Demonstration of natriuretic activity in urine of neurosurgical patients with renal salt wasting [v1; ref status: indexed, http://f1000r.es/nu

    Directory of Open Access Journals (Sweden)

    Steven J Youmans

    2013-05-01

    Full Text Available We have utilized the persistent elevation of fractional excretion (FE of urate, > 10%, to differentiate cerebral/renal salt wasting (RSW from the syndrome of inappropriate antidiuretic hormone secretion (SIADH, in which a normalization of FEurate occurs after correction of hyponatremia.  Previous studies suggest as well  that an elevated FEurate with normonatremia, without pre-existing hyponatremia, is also consistent with RSW, including studies demonstrating induction of RSW in rats infused with plasma from normonatremic neurosurgical and Alzheimer’s disease patients.  The present studies were designed to test whether precipitates from the urine of normonatremic neurosurgical patients, with either normal or elevated FEurate, and patients with SIADH, display natriuretic activity.   Methods: Ammonium sulfate precipitates from the urine of 6 RSW and 5 non-RSW Control patients were dialyzed (10 kDa cutoff to remove the ammonium sulfate, lyophilized, and the reconstituted precipitate was tested for its effect on transcellular transport of 22Na across LLC-PK1 cells grown to confluency in transwells. Results: Precipitates from 5 of the 6 patients with elevated FEurate and normonatremia significantly inhibited the in vitro transcellular transport of 22Na above a concentration of 3 μg protein/ml, by 10-25%, versus to vehicle alone, and by 15-40% at concentrations of 5-20 μg/ml as compared to precipitates from 4 of the 5 non-RSW patients with either normal FEurate and normonatremia (2 patients or with SIADH (2 patients. Conclusion: These studies provide further evidence that an elevated FEurate with normonatremia is highly consistent with RSW.  Evidence in the urine of natriuretic activity suggests significant renal excretion of the natriuretic factor. The potentially large source of the natriuretic factor that this could afford, coupled with small analytical sample sizes required by the in-vitro bioassay used here, should facilitate future

  16. Waste and Disposal: Demonstration

    International Nuclear Information System (INIS)

    Neerdael, B.; Buyens, M.; De Bruyn, D.; Volckaert, G.

    2002-01-01

    Within the Belgian R and D programme on geological disposal, demonstration experiments have become increasingly important. In this contribution to the scientific report 2001, an overview is given of SCK-CEN's activities and achievements in the field of large-scale demonstration experiments. In 2001, main emphasis was on the PRACLAY project, which is a large-scale experiment to demonstrate the construction and the operation of a gallery for the disposal of HLW in a clay formation. The PRACLAY experiment will contribute to enhance understanding of water flow and mass transport in dense clay-based materials as well as to improve the design of the reference disposal concept. In the context of PRACLAY, a surface experiment (OPHELIE) has been developed to prepare and to complement PRACLAY-related experimental work in the HADES Underground Research Laboratory. In 2001, efforts were focussed on the operation of the OPHELIE mock-up. SCK-CEN also contributed to the SELFRAC roject which studies the self-healing of fractures in a clay formation

  17. Phase I Clinical Trial of the CYP17 Inhibitor Abiraterone Acetate Demonstrating Clinical Activity in Patients With Castration-Resistant Prostate Cancer Who Received Prior Ketoconazole Therapy

    Science.gov (United States)

    Ryan, Charles J.; Smith, Matthew R.; Fong, Lawrence; Rosenberg, Jonathan E.; Kantoff, Philip; Raynaud, Florence; Martins, Vanessa; Lee, Gloria; Kheoh, Thian; Kim, Jennifer; Molina, Arturo; Small, Eric J.

    2010-01-01

    Purpose Abiraterone acetate is a prodrug of abiraterone, a selective inhibitor of CYP17, the enzyme catalyst for two essential steps in androgen biosynthesis. In castration-resistant prostate cancers (CRPCs), extragonadal androgen sources may sustain tumor growth despite a castrate environment. This phase I dose-escalation study of abiraterone acetate evaluated safety, pharmacokinetics, and effects on steroidogenesis and prostate-specific antigen (PSA) levels in men with CPRC with or without prior ketoconazole therapy. Patients and Methods Thirty-three men with chemotherapy-naïve progressive CRPC were enrolled. Nineteen patients (58%) had previously received ketoconazole for CRPC. Bone metastases were present in 70% of patients, and visceral involvement was present in 18%. Three patients (9%) had locally advanced disease without distant metastases. Fasted or fed cohorts received abiraterone acetate doses of 250, 500, 750, or 1,000 mg daily. Single-dose pharmacokinetic analyses were performed before continuous daily dosing. Results Adverse events were predominantly grade 1 or 2. No dose-limiting toxicities were observed. Hypertension (grade 3, 12%) and hypokalemia (grade 3, 6%; grade 4, 3%) were the most frequent serious toxicities and responded to medical management. Confirmed ≥ 50% PSA declines at week 12 were seen in 18 (55%) of 33 patients, including nine (47%) of 19 patients with prior ketoconazole therapy and nine (64%) of 14 patients without prior ketoconazole therapy. Substantial declines in circulating androgens and increases in mineralocorticoids were seen with all doses. Conclusion Abiraterone acetate was well tolerated and demonstrated activity in CRPC, including in patients previously treated with ketoconazole. Continued clinical study is warranted. PMID:20159824

  18. Buried Waste Integrated Demonstration lessons learned: 1993 technology demonstrations

    International Nuclear Information System (INIS)

    Kostelnik, K.M.; Owens, K.J.

    1994-01-01

    An integrated technology demonstration was conducted by the Buried Waste Integrated Demonstration (BWID) at the Idaho National Engineering Laboratory Cold Test Pit in the summer of 1993. This program and demonstration was sponsored by the US Department of Energy Office of Technology Development. The demonstration included six technologies representing a synergistic system for the characterization and retrieval of a buried hazardous waste site. The integrated technology demonstration proved very successful and a summary of the technical accomplishments is presented. Upon completion of the integrated technology demonstration, cognizant program personnel participated in a lessons learned exercise. This exercise was conducted at the Simplot Decision Support Center at Idaho State University and lessons learned activity captured additional information relative to the integration of technologies for demonstration purposes. This information will be used by BWID to enhance program planning and strengthen future technology demonstrations

  19. Inseparable phone books demonstration

    Science.gov (United States)

    Balta, Nuri; Çetin, Ali

    2017-05-01

    This study is aimed at first introducing a well-known discrepant event; inseparable phone books and second, turning it into an experiment for high school or middle school students. This discrepant event could be used especially to indicate how friction force can be effective in producing an unexpected result. Demonstration, discussion, explanation and experiment steps are presented on how to turn a simple discrepant event into an instructional activity. Results showed the relationships between number of pages and force, as well as between amounts of interleave and force. In addition to these, the mathematical equation for the total force between all interleaved pages is derived. As a conclusion, this study demonstrated that not only can phone books be used, but also ordinary books, to investigate this discrepant event. This experiment can be conducted as an example to show the agreement between theoretical and experimental results along with the confounding variables. This discrepant event can be used to create a cognitive conflict in students’ minds about the concepts of ‘force and motion’ and ‘friction force’.

  20. Mining amino acid association patterns in class B GPCRs.

    Science.gov (United States)

    Kumari, Tannu; Pardasani, Kamal Raj

    2015-01-01

    Class B GPCR family is a small group of receptors which are activated by peptides of intermediate length that range from 30 to 40 amino acid residues including hormones, neuropeptides and autocrine factors that mediate diverse physiological functions. They are involved in physiological processes like glucose homeostasis (glucagon and glucagon-like peptide-1), calcium homeostasis and bone turnover (parathyroid hormone and calcitonin), and control of the stress axis (corticotropin-releasing factor). Most of the GPCR structures and their functions are still unknown. Thus, the study of amino acid association patterns can be useful in prediction of their structure and functions. In view of above, in this paper, an attempt has been made to explore amino acid association patterns in class B GPCRs and their relationships with secondary structures and physiochemical properties. The fuzzy association rule mining is employed to take care of uncertainty due to variation in length of sequences. The association rules have been generated with the help of patterns discovered in the sequences.

  1. Exploration Medical System Demonstration

    Science.gov (United States)

    Rubin, D. A.; Watkins, S. D.

    2014-01-01

    BACKGROUND: Exploration class missions will present significant new challenges and hazards to the health of the astronauts. Regardless of the intended destination, beyond low Earth orbit a greater degree of crew autonomy will be required to diagnose medical conditions, develop treatment plans, and implement procedures due to limited communications with ground-based personnel. SCOPE: The Exploration Medical System Demonstration (EMSD) project will act as a test bed on the International Space Station (ISS) to demonstrate to crew and ground personnel that an end-to-end medical system can assist clinician and non-clinician crew members in optimizing medical care delivery and data management during an exploration mission. Challenges facing exploration mission medical care include limited resources, inability to evacuate to Earth during many mission phases, and potential rendering of medical care by non-clinicians. This system demonstrates the integration of medical devices and informatics tools for managing evidence and decision making and can be designed to assist crewmembers in nominal, non-emergent situations and in emergent situations when they may be suffering from performance decrements due to environmental, physiological or other factors. PROJECT OBJECTIVES: The objectives of the EMSD project are to: a. Reduce or eliminate the time required of an on-orbit crew and ground personnel to access, transfer, and manipulate medical data. b. Demonstrate that the on-orbit crew has the ability to access medical data/information via an intuitive and crew-friendly solution to aid in the treatment of a medical condition. c. Develop a common data management framework that can be ubiquitously used to automate repetitive data collection, management, and communications tasks for all activities pertaining to crew health and life sciences. d. Ensure crew access to medical data during periods of restricted ground communication. e. Develop a common data management framework that

  2. RADIOACTIVE DEMONSTRATIONS OF FLUIDIZED BED STEAM REFORMING AS A SUPPLEMENTARY TREATMENT FOR HANFORD'S LOW ACTIVITY WASTE AND SECONDARY WASTES

    Energy Technology Data Exchange (ETDEWEB)

    Jantzen, C.; Crawford, C.; Cozzi, A.; Bannochie, C.; Burket, P.; Daniel, G.

    2011-02-24

    The U.S. Department of Energy's Office of River Protection (ORP) is responsible for the retrieval, treatment, immobilization, and disposal of Hanford's tank waste. Currently there are approximately 56 million gallons of highly radioactive mixed wastes awaiting treatment. A key aspect of the River Protection Project (RPP) cleanup mission is to construct and operate the Waste Treatment and Immobilization Plant (WTP). The WTP will separate the tank waste into high-level and low-activity waste (LAW) fractions, both of which will subsequently be vitrified. The projected throughput capacity of the WTP LAW Vitrification Facility is insufficient to complete the RPP mission in the time frame required by the Hanford Federal Facility Agreement and Consent Order, also known as the Tri-Party Agreement (TPA), i.e. December 31, 2047. Therefore, Supplemental Treatment is required both to meet the TPA treatment requirements as well as to more cost effectively complete the tank waste treatment mission. The Supplemental Treatment chosen will immobilize that portion of the retrieved LAW that is not sent to the WTP's LAW Vitrification facility into a solidified waste form. The solidified waste will then be disposed on the Hanford site in the Integrated Disposal Facility (IDF). In addition, the WTP LAW vitrification facility off-gas condensate known as WTP Secondary Waste (WTP-SW) will be generated and enriched in volatile components such as Cs-137, I-129, Tc-99, Cl, F, and SO4 that volatilize at the vitrification temperature of 1150 C in the absence of a continuous cold cap. The current waste disposal path for the WTP-SW is to recycle it to the supplemental LAW treatment to avoid a large steady state accumulation in the pretreatment-vitrification loop. Fluidized Bed Steam Reforming (FBSR) offers a moderate temperature (700-750 C) continuous method by which LAW and/or WTP-SW wastes can be processed irrespective of whether they contain organics, nitrates, sulfates

  3. Demonstration and manifestation of self-determination and illness resistance--a qualitative study of long-term maintenance of physical activity in posttreatment cancer survivors

    DEFF Research Database (Denmark)

    Midtgaard, Julie; Røssell, Kasper; Christensen, Jesper Frank

    2012-01-01

    The aim of this study was to describe posttreatment cancer survivors' lived experience of long-term maintenance of physical activity (PA).......The aim of this study was to describe posttreatment cancer survivors' lived experience of long-term maintenance of physical activity (PA)....

  4. Radioactive Demonstration Of Mineralized Waste Forms Made From Hanford Low Activity Waste (Tank Farm Blend) By Fluidized Bed Steam Reformation (FBSR)

    Energy Technology Data Exchange (ETDEWEB)

    Jantzen, C. M. [Savannah River Site (SRS), Aiken, SC (United States). Savannah River National Lab. (SRNL); Crawford, C. L. [Savannah River Site (SRS), Aiken, SC (United States). Savannah River National Lab. (SRNL); Bannochie, C. J. [Savannah River Site (SRS), Aiken, SC (United States). Savannah River National Lab. (SRNL); Burket, P. R. [Savannah River Site (SRS), Aiken, SC (United States). Savannah River National Lab. (SRNL); Cozzi, A. D. [Savannah River Site (SRS), Aiken, SC (United States). Savannah River National Lab. (SRNL); Daniel, W. E. [Savannah River Site (SRS), Aiken, SC (United States). Savannah River National Lab. (SRNL); Hall, H. K. [Savannah River Site (SRS), Aiken, SC (United States). Savannah River National Lab. (SRNL); Miller, D. H. [Savannah River Site (SRS), Aiken, SC (United States). Savannah River National Lab. (SRNL); Missimer, D. M. [Savannah River Site (SRS), Aiken, SC (United States). Savannah River National Lab. (SRNL); Nash, C. A. [Savannah River Site (SRS), Aiken, SC (United States). Savannah River National Lab. (SRNL); Williams, M. F. [Savannah River Site (SRS), Aiken, SC (United States). Savannah River National Lab. (SRNL)

    2013-08-01

    The U.S. Department of Energy’s Office of River Protection (ORP) is responsible for the retrieval, treatment, immobilization, and disposal of Hanford’s tank waste. A key aspect of the River Protection Project (RPP) cleanup mission is to construct and operate the Hanford Tank Waste Treatment and Immobilization Plant (WTP). The WTP will separate the tank waste into high-level and low-activity waste (LAW) fractions, both of which will subsequently be vitrified. The projected throughput capacity of the WTP LAW Vitrification Facility is insufficient to complete the RPP mission in the time frame required by the Hanford Federal Facility Agreement and Consent Order, also known as the Tri-Party Agreement (TPA), i.e. December 31, 2047. Supplemental Treatment is likely to be required both to meet the TPA treatment requirements as well as to more cost effectively complete the tank waste treatment mission. The Supplemental Treatment chosen will immobilize that portion of the retrieved LAW that is not sent to the WTP’s LAW Vitrification facility into a solidified waste form. The solidified waste will then be disposed on the Hanford site in the Integrated Disposal Facility (IDF). Fluidized Bed Steam Reforming (FBSR) offers a moderate temperature (700-750°C) continuous method by which LAW can be processed irrespective of whether the waste contain organics, nitrates, sulfates/sulfides, chlorides, fluorides, volatile radionuclides or other aqueous components. The FBSR technology can process these wastes into a crystalline ceramic (mineral) waste form. The mineral waste form that is produced by co-processing waste with kaolin clay in an FBSR process has been shown to be comparable to LAW glass, i.e. leaches Tc-99, Re and Na at <2g/m2 during ASTM C1285 (Product Consistency) durability testing. Monolithing of the granular FBSR product was investigated to prevent dispersion during transport or burial/storage. Monolithing in an inorganic geopolymer binder, which is

  5. A quantitative histochemical procedure for the demonstration of purine nucleoside phosphorylase activity in rat and human liver using Tetranitro BT and xanthine oxidase as auxiliary enzyme

    NARCIS (Netherlands)

    Frederiks, W. M.; Bosch, K. S.; van Gulik, T.

    1993-01-01

    A quantitative histochemical procedure was developed for the demonstration of purine nucleoside phosphorylase in rat liver using unfixed cryostat sections and the auxiliary enzyme xanthine oxidase. The optimum incubation medium contained 18% (w/v) poly(vinyl alcohol), 100 mM phosphate buffer, pH

  6. Comparative Demonstration of Active and Semi-Passive In Situ Bioremediation Approaches for Perchlorate-Impacted Groundwater (Longhorn Army Ammunition Plant)

    Science.gov (United States)

    2009-01-01

    Positives were identified by the presence of PCR amplification products in agarose electrophoresis gels . Dechloromonas Dechlorosoma Site...chemical oxygen demand oC degrees Celsius Dem/Val demonstrate/validate DGGE denaturing gradient gel electrophoresis DHG Dissolved hydrocarbon...Status, and Remedial Options. September 2005. http://www.itrcweb.org/Documents/PERC-1. pdf • Interstate Technology & Regulatory Council Perchlorate Team

  7. Programme and activities on nuclear desalination in Morocco. Pre-project study on demonstration plant for seawater desalination using nuclear heating reactor in Morocco

    International Nuclear Information System (INIS)

    Righi, M.

    1998-01-01

    The first part of this paper gives the general information on the pre-project study of a demonstration plant for seawater desalination using a heating reactor being assessed jointly by Morocco and China. The progress of the pre-project study is elaborated in the second part. (author)

  8. Interim Letter Report - Verification Survey Results for Activities Performed in March 2009 for the Vitrification Test Facility Warehouse at the West Valley Demonstration Project, Ashford, New York

    Energy Technology Data Exchange (ETDEWEB)

    B.D. Estes

    2009-04-24

    The objective of the verification activities was to provide independent radiological surveys and data for use by the Department of Energy (DOE) to ensure that the building satisfies the requirements for release without radiological controls.

  9. Radioactive Demonstration Of Mineralized Waste Forms Made From Hanford Low Activity Waste (Tank Farm Blend) By Fluidized Bed Steam Reformation (FBSR)

    International Nuclear Information System (INIS)

    Jantzen, C. M.; Crawford, C. L.; Bannochie, C. J.; Burket, P. R.; Cozzi, A. D.; Daniel, W. E.; Hall, H. K.; Miller, D. H.; Missimer, D. M.; Nash, C. A.; Williams, M. F.

    2013-01-01

    The U.S. Department of Energy's Office of River Protection (ORP) is responsible for the retrieval, treatment, immobilization, and disposal of Hanford's tank waste. A key aspect of the River Protection Project (RPP) cleanup mission is to construct and operate the Hanford Tank Waste Treatment and Immobilization Plant (WTP). The WTP will separate the tank waste into high-level and low-activity waste (LAW) fractions, both of which will subsequently be vitrified. The projected throughput capacity of the WTP LAW Vitrification Facility is insufficient to complete the RPP mission in the time frame required by the Hanford Federal Facility Agreement and Consent Order, also known as the Tri-Party Agreement (TPA), i.e. December 31, 2047. Supplemental Treatment is likely to be required both to meet the TPA treatment requirements as well as to more cost effectively complete the tank waste treatment mission. The Supplemental Treatment chosen will immobilize that portion of the retrieved LAW that is not sent to the WTP's LAW Vitrification facility into a solidified waste form. The solidified waste will then be disposed on the Hanford site in the Integrated Disposal Facility (IDF). Fluidized Bed Steam Reforming (FBSR) offers a moderate temperature (700-750°C) continuous method by which LAW can be processed irrespective of whether the waste contain organics, nitrates, sulfates/sulfides, chlorides, fluorides, volatile radionuclides or other aqueous components. The FBSR technology can process these wastes into a crystalline ceramic (mineral) waste form. The mineral waste form that is produced by co-processing waste with kaolin clay in an FBSR process has been shown to be comparable to LAW glass, i.e. leaches Tc-99, Re and Na at 6 (the Hanford IDF criteria for Na) in the first few hours. The granular and monolithic waste forms also pass the EPA Toxicity Characteristic Leaching Procedure (TCLP) for all Resource Conservation and Recovery Act (RCRA) components at the Universal Treatment

  10. Demonstration of biological activities of extracts from Isodon rugosus Wall. Ex Benth: Separation and identification of bioactive phytoconstituents by GC-MS analysis in the ethyl acetate extract.

    Science.gov (United States)

    Zeb, Anwar; Ullah, Farhat; Ayaz, Muhammad; Ahmad, Sajjad; Sadiq, Abdul

    2017-05-30

    Since long, natural sources have been explored for possible managements of various diseases. In this context, the study is designed to evaluate Isodon rugosus Wall. ex Benth for biological potentials including antibacterial, anthelmintic, insecticidal, anti-termites and anti-Pharaoh activities followed by GC-MS analysis of active fraction to identify various bioactive compounds. I. rugosus was investigated against eight bacterial strains using well diffusion method and microdilution method with ceftriaxone as positive control. Similarly, the insecticidal activity was carried out against Tribolium castaneum, Rhyzopertha dominica, Monomorium pharaonis and Heterotermis indicola following contact toxicity method. Likewise, anthelmintic activity was performed against Ascaridia galli and Pherethima posthuma using albendazole as positive control, in which the paralysis and death times of the worms were observed. The GC-MS analysis of the most active solvent fraction was performed for identifications of various bioactive compounds. Among the tested samples of I. rugosus, flavonoids and ethyl acetate fraction exhibited high antibacterial activities. The crude saponins showed highest anthelmintic activity against Pherethima posthuma and Ascaridia galli with death times of 27.67 and 29.22 min respectively at concentrations of 40 mg/ml. In insecticidal activity, chloroform fraction and saponins exhibited notable results against R. dominica (60 and 70%) and T. castaneum (70 and 76%) at concentration of 200 mg/ml. In anti-termite assay, all the plant samples showed overwhelming results, i.e. all the 25 termites were killed on the 3rd day. Similarly, in anti-Pharaoh activity, the chloroform, ethyl acetate and saponins fractions were most potent, each exhibiting LD 50 of ethyl linolate, cyclohexanone, hinokione, methyl palmitate, ethyl palmitate and stigmasterol acetate. Based on our current results, it can be concluded that I. rugosus possess strong antibacterial, insecticidal

  11. Acetyl aspartic acid, a novel active ingredient, demonstrates potential to improve signs of skin ageing: from consumer need to clinical proof.

    Science.gov (United States)

    Mavon, A

    2015-10-01

    The megatrend of population ageing is leading to a growing demand for "anti-ageing" treatments, especially to prevent or treat skin ageing. Facing an increasing offer, consumers are choosing more and more skin care products supported by a scientific rationale, active ingredients and clinical proof of efficacy. Considering consumer expectations, this research led to the discovery of acetyl aspartic acid (A-A-A), a novel active ingredient to improve sagging skin and loss of skin firmness. This supplement is featuring seven manuscripts aiming at presenting the research and investigations from consumer insights, discovery of A-A-A, its in vitro activity confirmation, safety assessment, formulation and its dermal absorption to the clinical proof of efficacy, investigated through two pilots' double bind randomized and placebo controlled studies on photo-aged skin. This extensive research enabled us to discover A-A-A, as an active ingredient with potential to repair sign of skin ageing and supported by clinical proof of efficacy. This active ingredient will be soon launched in a commercial innovative skin care range, delivering desirable anti-wrinkle and skin lifting benefits. © 2015 Society of Cosmetic Scientists and the Société Française de Cosmétologie.

  12. Biodiesel Mass Transit Demonstration

    Science.gov (United States)

    2010-04-01

    The Biodiesel Mass Transit Demonstration report is intended for mass transit decision makers and fleet managers considering biodiesel use. This is the final report for the demonstration project implemented by the National Biodiesel Board under a gran...

  13. Authoring Effective Demonstrations

    National Research Council Canada - National Science Library

    Fu, Dan; Jensen, Randy; Salas, Eduardo; Rosen, Michael A; Ramachandran, Sowmya; Upshaw, Christin L; Hinkelman, Elizabeth; Lampton, Don

    2007-01-01

    ... or human role-players for each training event. We report our ongoing efforts to (1) research the nature and purpose of demonstration, articulating guidelines for effective demonstration within a training context, and (2...

  14. Demonstration exercise 'Cavtat 09'

    International Nuclear Information System (INIS)

    Trut, D.

    2009-01-01

    The demonstration exercise is to show a terrorist attack in urban area resulting in a certain number of injured people. On 7th April 2009 a terrorist group HAL 9000 is in Cavtat and set up an explosive devices with chemical reagents in several spots with intention to activate them and cause great number of victims. On the same day, in area of the Cavtat Croatia Hotel, which is hosting the world CBMTS Congress, Cavtat Police Station notice several masked persons, in escapement. Hotel personnel alerted the County 112 Center about noticed devices placed by chlorine dioxide tanks, for water conditioning. Intervention police came to block entrance to this area and evacuate hotel's guests and congress members. An explosion and fire occurs from where the position of water-conditioning plant and chlorine dioxide tank. The 112 Center alarms fire-fighters for fight fire and decontamination action and HAZMAT Civil Support Team from Georgia (participated the congress). In the meantime, guests have been instructed not to leave their rooms and to hermetically close doors and windows with available material to keep away potential toxic fume. Decision makers form the County Protection and Rescue Headquarters monitors the situation till the end of alert for the population in the area of Cavtat.(author)

  15. Inhibition of Plasmepsin V activity demonstrates its essential role in protein export, PfEMP1 display, and survival of malaria parasites

    DEFF Research Database (Denmark)

    Sleebs, Brad E; Lopaticki, Sash; Marapana, Danushka S

    2014-01-01

    , Plasmepsin V (PMV). The PMV gene is refractory to deletion, suggesting it is essential, but definitive proof is lacking. Here, we generated a PEXEL-mimetic inhibitor that potently blocks the activity of PMV isolated from P. falciparum and Plasmodium vivax. Assessment of PMV activity in P. falciparum revealed...... PEXEL cleavage occurs cotranslationaly, similar to signal peptidase. Treatment of P. falciparum-infected erythrocytes with the inhibitor caused dose-dependent inhibition of PEXEL processing as well as protein export, including impaired display of the major virulence adhesin, PfEMP1, on the erythrocyte...

  16. Strategy Guideline. Demonstration Home

    Energy Technology Data Exchange (ETDEWEB)

    Hunt, A.; Savage, C.

    2012-12-01

    This guideline will provide a general overview of the different kinds of demonstration home projects, a basic understanding of the different roles and responsibilities involved in the successful completion of a demonstration home, and an introduction into some of the lessons learned from actual demonstration home projects. Also, this guideline will specifically look at the communication methods employed during demonstration home projects. And lastly, we will focus on how to best create a communication plan for including an energy efficient message in a demonstration home project and carry that message to successful completion.

  17. Strategy Guideline: Demonstration Home

    Energy Technology Data Exchange (ETDEWEB)

    Savage, C.; Hunt, A.

    2012-12-01

    This guideline will provide a general overview of the different kinds of demonstration home projects, a basic understanding of the different roles and responsibilities involved in the successful completion of a demonstration home, and an introduction into some of the lessons learned from actual demonstration home projects. Also, this guideline will specifically look at the communication methods employed during demonstration home projects. And lastly, we will focus on how to best create a communication plan for including an energy efficient message in a demonstration home project and carry that message to successful completion.

  18. Tenofovir alafenamide demonstrates broad cross-genotype activity against wild-type HBV clinical isolates and maintains susceptibility to drug-resistant HBV isolates in vitro.

    Science.gov (United States)

    Liu, Yang; Miller, Michael D; Kitrinos, Kathryn M

    2017-03-01

    Tenofovir alafenamide (TAF) is a novel prodrug of tenofovir (TFV). This study evaluated the antiviral activity of TAF against wild-type genotype A-H HBV clinical isolates as well as adefovir-resistant, lamivudine-resistant, and entecavir-resistant HBV isolates. Full length HBV genomes or the polymerase/reverse transcriptase (pol/RT) region from treatment-naïve patients infected with HBV genotypes A-H were amplified and cloned into an expression vector under the control of a CMV promoter. In addition, 11 drug resistant HBV constructs were created by site-directed mutagenesis of a full length genotype D construct. Activity of TAF was measured by transfection of each construct into HepG2 cells and assessment of HBV DNA levels following treatment across a range of TAF concentrations. TAF activity in vitro was similar against wild-type genotype A-H HBV clinical isolates. All lamivudine- and entecavir-resistant isolates and 4/5 adefovir-resistant isolates were found to be sensitive to inhibition by TAF in vitro as compared to the wild-type isolate. The adefovir-resistant isolate rtA181V + rtN236T exhibited low-level reduced susceptibility to TAF. TAF is similarly active in vitro against wild-type genotype A-H HBV clinical isolates. The TAF sensitivity results for all drug-resistant isolates are consistent with what has been observed with the parent drug TFV. The in vitro cell-based HBV phenotyping assay results support the use of TAF in treatment of HBV infected subjects with diverse HBV genotypes, in both treatment-naive and treatment-experienced HBV infected patients. Copyright © 2016 Elsevier B.V. All rights reserved.

  19. Screening of antifungal azole drugs and agrochemicals with an adapted alamarBlue-based assay demonstrates antibacterial activity of croconazole against Mycobacterium ulcerans.

    Science.gov (United States)

    Scherr, Nicole; Röltgen, Katharina; Witschel, Matthias; Pluschke, Gerd

    2012-12-01

    An alamarBlue-based growth inhibition assay has been adapted for the thermosensitive and slow-growing pathogen Mycobacterium ulcerans. The standardized test procedure enables medium-throughput screening of preselected compound libraries. Testing of a set of 48 azoles with known antifungal activity led to the identification of an imidazole antifungal displaying an inhibitory dose (ID) of 9 μM for M. ulcerans.

  20. p.X654R IDUA variant among Thai individuals with intermediate mucopolysaccharidosis type I and its residual activity as demonstrated in COS-7 cells.

    Science.gov (United States)

    Ngiwsara, Lukana; Ketudat-Cairns, James R; Sawangareetrakul, Phannee; Charoenwattanasatien, Ratana; Champattanachai, Voraratt; Kuptanon, Chulaluck; Pangkanon, Suthipong; Tim-Aroon, Thipwimol; Wattanasirichaigoon, Duangrurdee; Svasti, Jisnuson

    2017-12-28

    Mucopolysaccharidosis type I (MPS I) is a rare autosomal-recessive disorder caused by defects in alpha-L-iduronidase (IDUA), a lysosomal enzyme encoded by the IDUA gene. Herein, we characterized IDUA mutations underlying mucopolysaccharidosis type I intermediate form (Hurler-Scheie syndrome) and its molecular pathogenic mechanisms. Clinical data, activity of the IDUA enzyme in leukocytes, and a mutation of the IDUA gene were analyzed. Pathogenesis associated with an IDUA mutation was further investigated by evaluating the mutant cDNA sequence, protein expression and activity in COS-7 cells. Five unrelated patients were identified to have clinical diagnosis of intermediate form of MPS I (Hurler-Scheie) and exhibited low-to-absent levels of leukocyte IDUA activity. Genetic analysis revealed homozygous c.*1T>C (p.X654R) mutation in two patients and compound heterozygosity between the c.*1T>C and another allele including c.265G>A (p.R89Q), c.935G>A (p.W312X), or c.1138 C>T (p.Q380X), each in a single patient. Sequencing the 3'RACE product of the c.*1T>C (p.X654R) allele indicated a 38-amino acids elongation of the mutant protein. COS-7 cells expressing IDUA with the mutations exhibited extremely low levels or complete absence of enzyme activity compared to wild-type IDUA. Western blot analysis detected no protein in p.W312X and p.Q380X samples, while an elevated molecular mass and a different pattern of protein bands were found in p.X654R specimen compared with the wild type IDUA. Mutational spectrum underlying intermediate MPS I is expanded. Our data suggest that the p.X654R is an intermediate IDUA mutant allele with residual enzyme activity. It can lead to intermediate or milder form of MPS I depending on another associated allele. © 2017 John Wiley & Sons Ltd/University College London.

  1. Demonstrating the use of high-volume electronic medical claims data to monitor local and regional influenza activity in the US.

    Directory of Open Access Journals (Sweden)

    Cécile Viboud

    Full Text Available Fine-grained influenza surveillance data are lacking in the US, hampering our ability to monitor disease spread at a local scale. Here we evaluate the performances of high-volume electronic medical claims data to assess local and regional influenza activity.We used electronic medical claims data compiled by IMS Health in 480 US locations to create weekly regional influenza-like-illness (ILI time series during 2003-2010. IMS Health captured 62% of US outpatient visits in 2009. We studied the performances of IMS-ILI indicators against reference influenza surveillance datasets, including CDC-ILI outpatient and laboratory-confirmed influenza data. We estimated correlation in weekly incidences, peak timing and seasonal intensity across datasets, stratified by 10 regions and four age groups (<5, 5-29, 30-59, and 60+ years. To test IMS-Health performances at the city level, we compared IMS-ILI indicators to syndromic surveillance data for New York City. We also used control data on laboratory-confirmed Respiratory Syncytial Virus (RSV activity to test the specificity of IMS-ILI for influenza surveillance.Regional IMS-ILI indicators were highly synchronous with CDC's reference influenza surveillance data (Pearson correlation coefficients rho≥0.89; range across regions, 0.80-0.97, P<0.001. Seasonal intensity estimates were weakly correlated across datasets in all age data (rho≤0.52, moderately correlated among adults (rho≥0.64 and uncorrelated among school-age children. IMS-ILI indicators were more correlated with reference influenza data than control RSV indicators (rho = 0.93 with influenza v. rho = 0.33 with RSV, P<0.05. City-level IMS-ILI indicators were highly consistent with reference syndromic data (rho≥0.86.Medical claims-based ILI indicators accurately capture weekly fluctuations in influenza activity in all US regions during inter-pandemic and pandemic seasons, and can be broken down by age groups and fine geographical areas

  2. Manufacturing Demonstration Facility (MDF)

    Data.gov (United States)

    Federal Laboratory Consortium — The U.S. Department of Energy Manufacturing Demonstration Facility (MDF) at Oak Ridge National Laboratory (ORNL) provides a collaborative, shared infrastructure to...

  3. Serum C-reactive Protein Levels Demonstrate Predictive Value for Radiographic and Magnetic Resonance Imaging Outcomes in Patients with Active Ankylosing Spondylitis Treated with Golimumab.

    Science.gov (United States)

    Braun, Jürgen; Baraliakos, Xenofon; Hermann, Kay-Geert A; Xu, Stephen; Hsu, Benjamin

    2016-09-01

    Serum C-reactive protein (CRP) associates with radiographic progression in patients with ankylosing spondylitis (AS) untreated with tumor necrosis factor (TNF) antagonists. We assessed correlations between serum CRP and radiographic progression/magnetic resonance imaging (MRI)-detected inflammation after 2 years of anti-TNF therapy. Patients with active AS receiving golimumab (GOL)/placebo through Week 16 (early escape) or Week 24 (crossover by design), followed by GOL through 4 years, had sera/images obtained through Week 208. Lateral spinal radiographs and spinal MRI were scored with the modified Stoke AS Spine Score (mSASSS) and the AS spine MRI activity (ASspiMRI-a) score, respectively. ANOVA assessed differences based on CRP levels and mSASSS progression. The relationships between CRP levels and mSASSS/ASspiMRI-a were assessed by Spearman correlation and logistic regression. Of the randomized GO-RAISE patients, 299 (84.0%) had pre- and posttreatment spinal radiographs. Larger proportions of patients with Week 104 CRP ≥ 0.5 mg/dl (n = 47) versus formation risk. Elevated CRP after 2 years of anti-TNF treatment correlated with greater radiographic progression risk at 4 years. Elevated CRP at baseline or Week 14/Week 24 of anti-TNF treatment weakly predicted subsequent radiographic progression and modestly predicted residual spinal inflammation in patients with AS treated with anti-TNF. Findings are useful regarding new treatment options in patients treated with anti-TNF. ClinicalTrials.gov: NCT00265083.

  4. Active vibration-based structural health monitoring system for wind turbine blade: Demonstration on an operating Vestas V27 wind turbine

    DEFF Research Database (Denmark)

    Tcherniak, Dmitri; Mølgaard, Lasse Lohilahti

    2017-01-01

    enough to be able to propagate the entire blade length. This article demonstrates the system on a Vestas V27 wind turbine. One blade of the wind turbine was equipped with the system, and a 3.5-month monitoring campaign was conducted while the turbine was operating normally. During the campaign, a defect......—a trailing-edge opening—was artificially introduced into the blade and its size was gradually increased from the original 15 to 45 cm. Using a semi-supervised learning algorithm, the system was able to detect even the smallest amount of damage while the wind turbine was operating under different weather......This study presents a structural health monitoring system that is able to detect structural defects of wind turbine blade such as cracks, leading/trailing-edge opening, or delamination. It is shown that even small defects of at least 15 cm size can be detected remotely without stopping the wind...

  5. My Most Annoying Demonstration

    Science.gov (United States)

    Mosca, Joseph M.

    2006-12-01

    After demonstrating various wave phenemona of pulses and continuous wavetrains in elastic media (slinky, springs, tuning forks etc.) students are still struggling with the concepts of standing waves, reflection and transmission at free and fixed boundaries, interference at nodes and antinodes, and the difference between transverse and longitudinal waves. In this simple demonstration we will overcome any misconceptions concerning these topics.

  6. Kinetics and Catalysis Demonstrations.

    Science.gov (United States)

    Falconer, John L.; Britten, Jerald A.

    1984-01-01

    Eleven videotaped kinetics and catalysis demonstrations are described. Demonstrations include the clock reaction, oscillating reaction, hydrogen oxidation in air, hydrogen-oxygen explosion, acid-base properties of solids, high- and low-temperature zeolite reactivity, copper catalysis of ammonia oxidation and sodium peroxide decomposition, ammonia…

  7. Attempt to demonstrate an in vivo effect of mianserin hydrochloride on erythrocyte Na+-K+-ATPase activity and cyclic AMP concentration

    Science.gov (United States)

    Naylor, G. S.; Buckley, D. E.; Boardman, L. J.; Smith, A. H. W.; Moody, J. P.

    1978-01-01

    1 There is evidence that erythrocyte Na+-K+-ATPase activity and erythrocyte cyclic AMP change on recovery from a depressive illness. Mianserin is a recently introduced antidepressant but its mode of action is unknown. The present study was therefore designed to investigate in vivo the effect of mianserin on erythrocyte Na+-K+-ATPase and cyclic AMP. 2 Biochemical estimations were made on blood from depressed patients before beginning either mianserin or matched placebo treatment, after 1 week, and again after 2 weeks' treatment. 3 Neither the erythrocyte Na+-K+-ATPase, nor the erythrocyte cyclic AMP concentration, changed significantly in either the mianserin- or the placebo-treated group. 4 The study sheds no light on the possible mechanism of action of mianserin. PMID:203308

  8. Geniposide demonstrates anti-inflammatory and antiviral activity against pandemic A/Jiangsu/1/2009 (H1N1) influenza virus infection in vitro and in vivo.

    Science.gov (United States)

    Zhang, Yunshi; Yao, Jing; Qi, Xian; Liu, Xing; Lu, Xieqin; Feng, Ganzhu

    2017-01-01

    Influenza A viruses (IAVs) have been a great threat to human health for centuries, without effective control. Geniposide, a main iridoid glycoside compound extracted from Gardenia jasminoides Ellis fruit, possesses various biological activities including anti-inflammation and anti-virus. Madin-Darby canine kidney (MDCK) cells were infected with pandemic A/Jiangsu/1/2009 (H1N1) influenza virus in vitro. Cytotoxicity and antiviral activity of geniposide were estimated by MTT assay. The influenza respiratory tract infection murine model was established by intranasal instillation of pandemic A/Jiangsu/1/2009 (H1N1) influenza virus. One day after infection, the mice were administered with geniposide (5, 10 or 20 mg/kg/day) or the neuraminidase inhibitor (NAI) peramivir (30 mg/kg/day). Body weight, survival time, viral titre and lung index of the mice were measured. The sandwich enzyme-linked immunosorbent assay (ELISA) was used to examine levels of inflammatory cytokines. The data showed that geniposide had little cytotoxicity on MDCK cells and protected them from pandemic A/Jiangsu/1/2009 (H1N1) influenza virus-induced cell injury. In the infected mice, geniposide treatment significantly restored the body weights, decreased the mortality, alleviated viral titres and virus-induced lung lesions. Geniposide substantially inhibited the virus-induced alveolar wall changes, alveolar haemorrhage and neutrophil-infiltration in lung tissues. Levels of inflammatory mediators, including tumour necrosis factor-α (TNF-α), interferon-γ (IFN-γ), interleukin (IL)-4, IL-6 and IL-10 were also markedly altered after treatment with geniposide. Our investigation suggested that geniposide effectively inhibited cell damage mediated by pandemic A/Jiangsu/1/2009 (H1N1) influenza virus and mitigated virus-induced acute inflammation.

  9. Long-Term Demonstration of Hydrogen Production from Coal at Elevated Temperatures Year 6 - Activity 1.12 - Development of a National Center for Hydrogen Technology

    Energy Technology Data Exchange (ETDEWEB)

    Stanislowski, Joshua; Tolbert, Scott; Curran, Tyler; Swanson, Michael

    2012-04-30

    The Energy & Environmental Research Center (EERC) has continued the work of the National Center for Hydrogen Technology® (NCHT®) Program Year 6 Task 1.12 project to expose hydrogen separation membranes to coal-derived syngas. In this follow-on project, the EERC has exposed two membranes to coal-derived syngas produced in the pilot-scale transport reactor development unit (TRDU). Western Research Institute (WRI), with funding from the State of Wyoming Clean Coal Technology Program and the North Dakota Industrial Commission, contracted with the EERC to conduct testing of WRI’s coal-upgrading/gasification technology for subbituminous and lignite coals in the EERC’s TRDU. This gasifier fires nominally 200–500 lb/hour of fuel and is the pilot-scale version of the full-scale gasifier currently being constructed in Kemper County, Mississippi. A slipstream of the syngas was used to demonstrate warm-gas cleanup and hydrogen separation using membrane technology. Two membranes were exposed to coal-derived syngas, and the impact of coal-derived impurities was evaluated. This report summarizes the performance of WRI’s patent-pending coalupgrading/ gasification technology in the EERC’s TRDU and presents the results of the warm-gas cleanup and hydrogen separation tests. Overall, the WRI coal-upgrading/gasification technology was shown to produce a syngas significantly lower in CO2 content and significantly higher in CO content than syngas produced from the raw fuels. Warm-gas cleanup technologies were shown to be capable of reducing sulfur in the syngas to 1 ppm. Each of the membranes tested was able to produce at least 2 lb/day of hydrogen from coal-derived syngas.

  10. Factors Influencing Spanish Instructors' In-Class Feedback Decisions

    Science.gov (United States)

    Gurzynski-Weiss, Laura

    2016-01-01

    While oral corrective feedback is a principal focus in second language acquisition research, most studies examine feedback once it has been provided. Investigating how instructors make in-class feedback decisions has not been thoroughly explored, despite the fact that classroom feedback occurs at the discretion of the individual language…

  11. Breaking sitting with light activities vs structured exercise: a randomised crossover study demonstrating benefits for glycaemic control and insulin sensitivity in type 2 diabetes.

    Science.gov (United States)

    Duvivier, Bernard M F M; Schaper, Nicolaas C; Hesselink, Matthijs K C; van Kan, Linh; Stienen, Nathalie; Winkens, Bjorn; Koster, Annemarie; Savelberg, Hans H C M

    2017-03-01

    We aimed to examine the effects of breaking sitting with standing and light-intensity walking vs an energy-matched bout of structured exercise on 24 h glucose levels and insulin resistance in patients with type 2 diabetes. In a randomised crossover study, 19 patients with type 2 diabetes (13 men/6 women, 63 ± 9 years old) who were not using insulin each followed three regimens under free-living conditions, each lasting 4 days: (1) Sitting: 4415 steps/day with 14 h sitting/day; (2) Exercise: 4823 steps/day with 1.1 h/day of sitting replaced by moderate- to vigorous-intensity cycling (at an intensity of 5.9 metabolic equivalents [METs]); and (3) Sit Less: 17,502 steps/day with 4.7 h/day of sitting replaced by standing and light-intensity walking (an additional 2.5 h and 2.2 h, respectively, compared with the hours spent doing these activities in the Sitting regimen). Blocked randomisation was performed using a block size of six regimen orders using sealed, non-translucent envelopes. Individuals who assessed the outcomes were blinded to group assignment. Meals were standardised during each intervention. Physical activity and glucose levels were assessed for 24 h/day by accelerometry (activPAL) and a glucose monitor (iPro2), respectively. The incremental AUC (iAUC) for 24 h glucose (primary outcome) and insulin resistance (HOMA2-IR) were assessed on days 4 and 5, respectively. The iAUC for 24 h glucose (mean ± SEM) was significantly lower during the Sit Less intervention than in Sitting (1263 ± 189 min × mmol/l vs 1974 ± 324 min × mmol/l; p = 0.002), and was similar between Sit Less and Exercise (Exercise: 1383 ± 194 min × mmol/l; p = 0.499). Exercise failed to improve HOMA2-IR compared with Sitting (2.06 ± 0.28 vs 2.16 ± 0.26; p = 0.177). In contrast, Sit Less (1.89 ± 0.26) significantly reduced HOMA2-IR compared with Exercise (p = 0.015) as well as Sitting (p = 0.001). Breaking

  12. Demonstrating usefulness of real-time monitoring at streambank wells coupled with active streamgages - Pilot studies in Wyoming, Montana, and Mississippi

    Science.gov (United States)

    Eddy-Miller, Cheryl A.; Constantz, Jim; Wheeler, Jerrod D.; Caldwell, Rodney R.; Barlow, Jeannie R.B.

    2012-01-01

    Groundwater and surface water in many cases are considered separate resources, but there is growing recognition of a need to treat them as a single resource. For example, groundwater inflow during low streamflow is vitally important to the health of a stream for many reasons, including buffering temperature, providing good quality water to the stream, and maintaining flow for aquatic organisms. The U.S. Geological Survey (USGS) has measured stream stage and flow at thousands of locations since 1889 and has the ability to distribute the information to the public within hours of collection, but collecting shallow groundwater data at co-located measuring sites is a new concept. Recently developed techniques using heat as a tracer to quantify groundwater and surface-water exchanges have shown the value of coupling these resources to increase the understanding of the water resources of an area. In 2009, the USGS Office of Groundwater began a pilot study to examine the feasibility and utility of widespread use of real-time groundwater monitoring at streambank wells coupled with real-time surface-water monitoring at active streamgages to assist in understanding the exchange of groundwater and surface water in a cost effective manner.

  13. Radioactive Demonstration Of Mineralized Waste Forms Made From Hanford Low Activity Waste (Tank SX-105 And AN-103) By Fluidized Bed Steam Reformation

    Energy Technology Data Exchange (ETDEWEB)

    Jantzen, Carol; Herman, Connie; Crawford, Charles; Bannochie, Christopher; Burket, Paul; Daniel, Gene; Cozzi, Alex; Nash, Charles; Miller, Donald; Missimer, David

    2014-01-10

    One of the immobilization technologies under consideration as a Supplemental Treatment for Hanford’s Low Activity Waste (LAW) is Fluidized Bed Steam Reforming (FBSR). The FBSR technology forms a mineral waste form at moderate processing temperatures thus retaining and atomically bonding the halides, sulfates, and technetium in the mineral phases (nepheline, sodalite, nosean, carnegieite). Additions of kaolin clay are used instead of glass formers and the minerals formed by the FBSR technology offers (1) atomic bonding of the radionuclides and constituents of concern (COC) comparable to glass, (2) short and long term durability comparable to glass, (3) disposal volumes comparable to glass, and (4) higher Na2O and SO{sub 4} waste loadings than glass. The higher FBSR Na{sub 2}O and SO{sub 4} waste loadings contribute to the low disposal volumes but also provide for more rapid processing of the LAW. Recent FBSR processing and testing of Hanford radioactive LAW (Tank SX-105 and AN-103) waste is reported and compared to previous radioactive and non-radioactive LAW processing and testing.

  14. IMO-8400, a toll-like receptor 7, 8, and 9 antagonist, demonstrates clinical activity in a phase 2a, randomized, placebo-controlled trial in patients with moderate-to-severe plaque psoriasis.

    Science.gov (United States)

    Balak, Deepak M W; van Doorn, Martijn B A; Arbeit, Robert D; Rijneveld, Rianne; Klaassen, Erica; Sullivan, Tim; Brevard, Julie; Thio, Hok Bing; Prens, Errol P; Burggraaf, Jacobus; Rissmann, Robert

    2017-01-01

    Aberrant toll-like receptors (TLRs) 7, 8, and 9 activation by self-nucleic acids is implicated in immune-mediated inflammatory diseases (IMIDs) such as psoriasis. In preclinical IMID models, blocking TLR-activation reduced disease severity. IMO-8400 is a first-in-class, oligonucleotide-based antagonist of TLRs 7, 8, and 9. We evaluated the short-term safety and proof-of-concept for efficacy of IMO-8400 in a first-in-patient phase 2 trial. Forty-six psoriasis patients were randomly assigned to IMO-8400 in four dose levels or placebo for 12weeks. Post-treatment follow-up was seven weeks. Primary outcome was incidence of adverse events. Secondary, exploratory outcomes included changes in psoriasis area and severity index (PASI). IMO-8400 across all dose levels did not cause any serious or severe adverse events. The most common treatment-related adverse events were dose-dependent injection-site reactions. All IMO-8400 groups showed clinical improvement, but a clear dose-response relationship and statistically significant differences with placebo were not observed (P=0.26). Eleven (38%) of 29 subjects on IMO-8400 achieved ≥50% PASI-reduction, compared to 1 (11%) of 9 subjects on placebo. Five (17%) and 2 (7%) IMO-8400-treated subjects achieved PASI-75 and PASI-90, respectively, compared to none on placebo. Short-term IMO-8400-treatment was well tolerated and reduced psoriasis severity. These findings warrant further investigation of endosomal TLR-antagonism as a therapeutic approach in psoriasis and other TLR-mediated IMIDs. EudraCT 2013-000164-28 and Clinicaltrials.govNCT01899729. Copyright © 2016 Elsevier Inc. All rights reserved.

  15. Non-clinical and Pre-clinical Testing to Demonstrate Safety of the Barostim Neo Electrode for Activation of Carotid Baroreceptors in Chronic Human Implants.

    Science.gov (United States)

    Wilks, Seth J; Hara, Seth A; Ross, Erika K; Nicolai, Evan N; Pignato, Paul A; Cates, Adam W; Ludwig, Kip A

    2017-01-01

    The Barostim neo™ electrode was developed by CVRx, Inc.to deliver baroreflex activation therapy (BAT)™ to treat hypertension and heart failure. The neo electrode concept was designed to deliver electrical stimulation to the baroreceptors within the carotid sinus bulb, while minimizing invasiveness of the implant procedure. This device is currently CE marked in Europe, and in a Pivotal (akin to Phase III) Trial in the United States. Here we present the in vitro and in vivo safety testing that was completed in order to obtain necessary regulatory approval prior to conducting human studies in Europe, as well as an FDA Investigational Device Exemption (IDE) to conduct a Pivotal Trial in the United States. Stimulated electrodes (10 mA, 500 μs, 100 Hz) were compared to unstimulated electrodes using optical microscopy and several electrochemical techniques over the course of 27 weeks. Electrode dissolution was evaluated by analyzing trace metal content of solutions in which electrodes were stimulated. Lastly, safety testing under Good Laboratory Practice guidelines was conducted in an ovine animal model over a 12 and 24 week time period, with results processed and evaluated by an independent histopathologist. Long-term stimulation testing indicated that the neo electrode with a sputtered iridium oxide coating can be stimulated at maximal levels for the lifetime of the implant without clinically significant dissolution of platinum or iridium, and without increasing the potential at the electrode interface to cause hydrolysis or significant tissue damage. Histological examination of tissue that was adjacent to the neo electrodes indicated no clinically significant signs of increased inflammation and no arterial stenosis as a result of 6 months of continuous stimulation. The work presented here involved rigorous characterization and evaluation testing of the neo electrode, which was used to support its safety for chronic implantation. The testing strategies discussed

  16. Comparing Demonstratives in Kwa

    African Journals Online (AJOL)

    nt'. The following sub-sections discuss the categories of demonstratives in the various languages in more detail. 3.1. Akan ...... languages: a classification and description of Africa s largest family,. 216-245. Lanham: University Press of America.

  17. Laser Communications Relay Demonstration

    Data.gov (United States)

    National Aeronautics and Space Administration — LCRD is a minimum two year flight demonstration in geosynchronous Earth orbit to advance optical communications technology toward infusion into Deep Space and Near...

  18. Spacecraft Fire Safety Demonstration

    Data.gov (United States)

    National Aeronautics and Space Administration — The objective of the Spacecraft Fire Safety Demonstration project is to develop and conduct large-scale fire safety experiments on an International Space Station...

  19. Education Payload Operation - Demonstrations

    Science.gov (United States)

    Keil, Matthew

    2009-01-01

    Education Payload Operation - Demonstrations (EPO-Demos) are recorded video education demonstrations performed on the International Space Station (ISS) by crewmembers using hardware already onboard the ISS. EPO-Demos are videotaped, edited, and used to enhance existing NASA education resources and programs for educators and students in grades K-12. EPO-Demos are designed to support the NASA mission to inspire the next generation of explorers.

  20. Edible Astronomy Demonstrations

    Science.gov (United States)

    Lubowich, Donald A.

    2007-12-01

    Astronomy demonstrations with edible ingredients are an effective way to increase student interest and knowledge of astronomical concepts. This approach has been successful with all age groups from elementary school through college students - and the students remember these demonstrations after they are presented. In this poster I describe edible demonstrations I have created to simulate the expansion of the universe (using big-bang chocolate chip cookies); differentiation during the formation of the Earth and planets (using chocolate or chocolate milk with marshmallows, cereal, candy pieces or nuts); and radioactivity/radioactive dating (using popcorn). Other possible demonstrations include: plate tectonics (crackers with peanut butter and jelly); convection (miso soup or hot chocolate); mud flows on Mars (melted chocolate poured over angel food cake); formation of the Galactic disk (pizza); formation of spiral arms (coffee with cream); the curvature of Space (Pringles); constellations patterns with chocolate chips and chocolate chip cookies; planet shaped cookies; star shaped cookies with different colored frostings; coffee or chocolate milk measurement of solar radiation; Oreo cookie lunar phases. Sometimes the students eat the results of the astronomical demonstrations. These demonstrations are an effective teaching tool and can be adapted for cultural, culinary, and ethnic differences among the students.

  1. The Anti-sigma Factor RsiV Is a Bacterial Receptor for Lysozyme: Co-crystal Structure Determination and Demonstration That Binding of Lysozyme to RsiV Is Required for σV Activation.

    Directory of Open Access Journals (Sweden)

    Jessica L Hastie

    2016-09-01

    Full Text Available σ factors provide RNA polymerase with promoter specificity in bacteria. Some σ factors require activation in order to interact with RNA polymerase and transcribe target genes. The Extra-Cytoplasmic Function (ECF σ factor, σV, is encoded by several Gram-positive bacteria and is specifically activated by lysozyme. This activation requires the proteolytic destruction of the anti-σ factor RsiV via a process of regulated intramembrane proteolysis (RIP. In many cases proteases that cleave at site-1 are thought to directly sense a signal and initiate the RIP process. We previously suggested binding of lysozyme to RsiV initiated the proteolytic destruction of RsiV and activation of σV. Here we determined the X-ray crystal structure of the RsiV-lysozyme complex at 2.3 Å which revealed that RsiV and lysozyme make extensive contacts. We constructed RsiV mutants with altered abilities to bind lysozyme. We find that mutants that are unable to bind lysozyme block site-1 cleavage of RsiV and σV activation in response to lysozyme. Taken together these data demonstrate that RsiV is a receptor for lysozyme and binding of RsiV to lysozyme is required for σV activation. In addition, the co-structure revealed that RsiV binds to the lysozyme active site pocket. We provide evidence that in addition to acting as a sensor for the presence of lysozyme, RsiV also inhibits lysozyme activity. Thus we have demonstrated that RsiV is a protein with multiple functions. RsiV inhibits σV activity in the absence of lysozyme, RsiV binds lysozyme triggering σV activation and RsiV inhibits the enzymatic activity of lysozyme.

  2. The Anti-sigma Factor RsiV Is a Bacterial Receptor for Lysozyme: Co-crystal Structure Determination and Demonstration That Binding of Lysozyme to RsiV Is Required for σV Activation.

    Science.gov (United States)

    Hastie, Jessica L; Williams, Kyle B; Bohr, Lindsey L; Houtman, Jon C; Gakhar, Lokesh; Ellermeier, Craig D

    2016-09-01

    σ factors provide RNA polymerase with promoter specificity in bacteria. Some σ factors require activation in order to interact with RNA polymerase and transcribe target genes. The Extra-Cytoplasmic Function (ECF) σ factor, σV, is encoded by several Gram-positive bacteria and is specifically activated by lysozyme. This activation requires the proteolytic destruction of the anti-σ factor RsiV via a process of regulated intramembrane proteolysis (RIP). In many cases proteases that cleave at site-1 are thought to directly sense a signal and initiate the RIP process. We previously suggested binding of lysozyme to RsiV initiated the proteolytic destruction of RsiV and activation of σV. Here we determined the X-ray crystal structure of the RsiV-lysozyme complex at 2.3 Å which revealed that RsiV and lysozyme make extensive contacts. We constructed RsiV mutants with altered abilities to bind lysozyme. We find that mutants that are unable to bind lysozyme block site-1 cleavage of RsiV and σV activation in response to lysozyme. Taken together these data demonstrate that RsiV is a receptor for lysozyme and binding of RsiV to lysozyme is required for σV activation. In addition, the co-structure revealed that RsiV binds to the lysozyme active site pocket. We provide evidence that in addition to acting as a sensor for the presence of lysozyme, RsiV also inhibits lysozyme activity. Thus we have demonstrated that RsiV is a protein with multiple functions. RsiV inhibits σV activity in the absence of lysozyme, RsiV binds lysozyme triggering σV activation and RsiV inhibits the enzymatic activity of lysozyme.

  3. Offsite demonstrations for MWLID technologies

    International Nuclear Information System (INIS)

    Williams, C.; Gruebel, R.

    1995-01-01

    The goal of the Offsite Demonstration Project for Mixed Waste Landfill Integrated Demonstration (MWLID)-developed environmental site characterization and remediation technologies is to facilitate the transfer, use, and commercialization of these technologies to the public and private sector. The meet this goal, the project identified environmental restoration needs of mixed waste and/or hazardous waste landfill owners (Native American, municipal, DOE, and DoD); documenting potential demonstration sites and the contaminants present at each site; assessing the environmental regulations that would effect demonstration activities; and evaluating site suitability for demonstrating MWLID technologies at the tribal and municipal sites identified. Eighteen landfill sites within a 40.2-km radius of Sandia National Laboratories are listed on the CERCLIS Site/Event Listing for the state of New Mexico. Seventeen are not located within DOE or DoD facilities and are potential offsite MWLID technology demonstration sites. Two of the seventeen CERCLIS sites, one on Native American land and one on municipal land, were evaluated and identified as potential candidates for off-site demonstrations of MWLID-developed technologies. Contaminants potentially present on site include chromium waste, household/commercial hazardous waste, volatile organic compounds, and petroleum products. MWLID characterization technologies applicable to these sites include Magnetometer Towed Array, Cross-borehole Electromagnetic Imaging, SitePlanner trademark/PLUME, Hybrid Directional Drilling, Seamist trademark/Vadose Zone Monitoring, Stripping Analyses, and x-ray Fluorescence Spectroscopy for Heavy Metals

  4. Demonstrating Operating System Principles via Computer Forensics Exercises

    Science.gov (United States)

    Duffy, Kevin P.; Davis, Martin H., Jr.; Sethi, Vikram

    2010-01-01

    We explore the feasibility of sparking student curiosity and interest in the core required MIS operating systems course through inclusion of computer forensics exercises into the course. Students were presented with two in-class exercises. Each exercise demonstrated an aspect of the operating system, and each exercise was written as a computer…

  5. Solar renovation demonstration projects

    Energy Technology Data Exchange (ETDEWEB)

    Bruun Joergensen, O. [ed.

    1998-10-01

    In the framework of the IEA SHC Programme, a Task on building renovation was initiated, `Task 20, Solar Energy in Building Renovation`. In a part of the task, Subtask C `Design of Solar Renovation Projects`, different solar renovation demonstration projects were developed. The objective of Subtask C was to demonstrate the application of advanced solar renovation concepts on real buildings. This report documents 16 different solar renovation demonstration projects including the design processes of the projects. The projects include the renovation of houses, schools, laboratories, and factories. Several solar techniques were used: building integrated solar collectors, glazed balconies, ventilated solar walls, transparent insulation, second skin facades, daylight elements and photovoltaic systems. These techniques are used in several simple as well as more complex system designs. (au)

  6. TRUEX hot demonstration

    International Nuclear Information System (INIS)

    Chamberlain, D.B.; Leonard, R.A.; Hoh, J.C.; Gay, E.C.; Kalina, D.G.; Vandegrift, G.F.

    1990-04-01

    In FY 1987, a program was initiated to demonstrate technology for recovering transuranic (TRU) elements from defense wastes. This hot demonstration was to be carried out with solution from the dissolution of irradiated fuels. This recovery would be accomplished with both PUREX and TRUEX solvent extraction processes. Work planned for this program included preparation of a shielded-cell facility for the receipt and storage of spent fuel from commercial power reactors, dissolution of this fuel, operation of a PUREX process to produce specific feeds for the TRUEX process, operation of a TRUEX process to remove residual actinide elements from PUREX process raffinates, and processing and disposal of waste and product streams. This report documents the work completed in planning and starting up this program. It is meant to serve as a guide for anyone planning similar demonstrations of TRUEX or other solvent extraction processing in a shielded-cell facility

  7. Inspection quality demonstrations

    International Nuclear Information System (INIS)

    Dau, G.J.

    1985-01-01

    This paper discusses an inspection demonstration process that was initiated in late 1982 and is still functioning. In 1982, the Nine Mile Point-1 boiling water reactor completed the required inspection governed by rules established by the American Society of Mechanical Engineers Boiler and Pressure Vessel Code and the U.S. Nuclear Regulatory Commission. The inspection results indicated there were no cracks when the results were evaluated against the established criteria. Later, while performing the system hydro-test prior to returning to power, one of the joints leaked, indicating a through-wall crack. The demonstration process, including a training program recognized by the NRC, are described in the paper. The final answer on how good the demonstration and field inspection are can only be determined by comparison of the inspection results with direct observation of the flaws. This is currently in progress

  8. Innovative technology demonstrations

    International Nuclear Information System (INIS)

    Anderson, D.B.; Luttrell, S.P.; Hartley, J.N.

    1992-08-01

    Environmental Management Operations (EMO) is conducting an Innovative Technology Demonstration Program for Tinker Air Force Base (TAFB). Several innovative technologies are being demonstrated to address specific problems associated with remediating two contaminated test sites at the base. Cone penetrometer testing (CPT) is a form of testing that can rapidly characterize a site. This technology was selected to evaluate its applicability in the tight clay soils and consolidated sandstone sediments found at TAFB. Directionally drilled horizontal wells was selected as a method that may be effective in accessing contamination beneath Building 3001 without disrupting the mission of the building, and in enhancing the extraction of contamination both in ground water and in soil. A soil gas extraction (SGE) demonstration, also known as soil vapor extraction, will evaluate the effectiveness of SGE in remediating fuels and TCE contamination contained in the tight clay soil formations surrounding the abandoned underground fuel storage vault located at the SW Tanks Site. In situ sensors have recently received much acclaim as a technology that can be effective in remediating hazardous waste sites. Sensors can be useful for determining real-time, in situ contaminant concentrations during the remediation process for performance monitoring and in providing feedback for controlling the remediation process. Following the SGE demonstration, the SGE system and SW Tanks test site will be modified to demonstrate bioremediation as an effective means of degrading the remaining contaminants in situ. The bioremediation demonstration will evaluate a bioventing process in which the naturally occurring consortium of soil bacteria will be stimulated to aerobically degrade soil contaminants, including fuel and TCE, in situ

  9. Gigashot Optical Laser Demonstrator

    Energy Technology Data Exchange (ETDEWEB)

    Deri, R. J. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)

    2015-10-13

    The Gigashot Optical Laser Demonstrator (GOLD) project has demonstrated a novel optical amplifier for high energy pulsed lasers operating at high repetition rates. The amplifier stores enough pump energy to support >10 J of laser output, and employs conduction cooling for thermal management to avoid the need for expensive and bulky high-pressure helium subsystems. A prototype amplifier was fabricated, pumped with diode light at 885 nm, and characterized. Experimental results show that the amplifier provides sufficient small-signal gain and sufficiently low wavefront and birefringence impairments to prove useful in laser systems, at repetition rates up to 60 Hz.

  10. Demonstrating marketing accountability.

    Science.gov (United States)

    Gombeski, William R; Britt, Jason; Taylor, Jan; Riggs, Karen; Wray, Tanya; Adkins, Wanda; Springate, Suzanne

    2008-01-01

    Pressure on health care marketers to demonstrate effectiveness of their strategies and show their contribution to organizational goals is growing. A seven-tiered model based on the concepts of structure (having the right people, systems), process (doing the right things in the right way), and outcomes (results) is discussed. Examples of measures for each tier are provided and the benefits of using the model as a tool for measuring, organizing, tracking, and communicating appropriate information are provided. The model also provides a framework for helping management understand marketing's value and can serve as a vehicle for demonstrating marketing accountability.

  11. Obese individuals with more components of the metabolic syndrome and/or prediabetes demonstrate decreased activation of reward-related brain centers in response to food cues in both the fed and fasting states: a preliminary fMRI study.

    Science.gov (United States)

    Farr, O M; Mantzoros, C S

    2017-03-01

    It remains unknown whether obese individuals with more components of the metabolic syndrome and/or prediabetes demonstrate altered activation of brain centers in response to food cues. We examined obese individuals with prediabetes (n=26) vs obese individuals without prediabetes (n=11) using fMRI. We also performed regression analyses on the basis of the number of MetS components per subject. Obese individuals with prediabetes have decreased activation of the reward-related putamen in the fasting state and decreased activation of the salience- and reward-related insula after eating. Obese individuals with more components of MetS demonstrate decreased activation of the putamen while fasting. All these activations remain significant when corrected for BMI, waist circumference (WC), HbA1c and gender. Decreased activation in the reward-related central nervous system areas among the obese is more pronounced in subjects with prediabetes and MetS. Prospective studies are needed to quantify their contributions to the development of prediabetes/MetS and to study whether they may predispose to the exacerbation of obesity and the development of comorbidities over time.

  12. Do Enthalpy and Entropy Distinguish First in Class From Best in Class?

    Science.gov (United States)

    Freire, Ernesto

    2008-01-01

    A drug molecule should bind to its target with high affinity and selectivity. Since the binding affinity is a combined function of the binding enthalpy and the binding entropy, extremely high affinity requires that both terms contribute favorably to binding. The binding enthalpy, however, is notoriously more difficult to optimize than the binding entropy, a fact that has resulted in thermodynamically-unbalanced molecules that do not achieve optimal potency. In fact, with current technologies, the enthalpic optimization of drug candidates may take years and only appear in second-generation products. Within that context, it is not surprising that structure/activity relationships (SAR) that explicitly incorporate the interplay between enthalpy and entropy and accelerate the optimization process are being developed and gaining popularity. PMID:18703160

  13. Organic Lecture Demonstrations.

    Science.gov (United States)

    Silversmith, Ernest F.

    1988-01-01

    Provides a listing of 35 demonstrations designed to generate interest in organic chemistry and help put points across. Topics include opening lecture; molecular structure and properties; halogenation; nucleophilic substitution, alkenes and dienes, stereochemistry, spectroscopy, alcohols and phenols, aldehydes and ketones; carboxylic acids, amines,…

  14. Participatory Lecture Demonstrations.

    Science.gov (United States)

    Battino, Rubin

    1979-01-01

    The use of participatory lecture demonstrations in the classroom is described. Examples are given for the following topics: chromatography, chemical kinetics, balancing equations, the gas laws, kinetic molecular theory, Henry's law of gas solubility, electronic energy levels in atoms, and translational, vibrational, and rotational energies of…

  15. Demonstrating the Gas Laws.

    Science.gov (United States)

    Holko, David A.

    1982-01-01

    Presents a complete computer program demonstrating the relationship between volume/pressure for Boyle's Law, volume/temperature for Charles' Law, and volume/moles of gas for Avagadro's Law. The programing reinforces students' application of gas laws and equates a simulated moving piston to theoretical values derived using the ideal gas law.…

  16. Monty Roberts’ public demonstrations

    NARCIS (Netherlands)

    Loftus, Loni; Marks, Kelly; Jones-McVey, Rosie; Gonzales, Jose L.; Fowler, Veronica L.

    2016-01-01

    Effective training of horses relies on the trainer’s awareness of learning theory and equine ethology, and should be undertaken with skill and time. Some trainers, such as Monty Roberts, share their methods through the medium of public demonstrations. This paper describes the opportunistic

  17. A Magnetic Circuit Demonstration.

    Science.gov (United States)

    Vanderkooy, John; Lowe, June

    1995-01-01

    Presents a demonstration designed to illustrate Faraday's, Ampere's, and Lenz's laws and to reinforce the concepts through the analysis of a two-loop magnetic circuit. Can be made dramatic and challenging for sophisticated students but is suitable for an introductory course in electricity and magnetism. (JRH)

  18. AVNG system demonstration

    Energy Technology Data Exchange (ETDEWEB)

    Thron, Jonathan Louis [Los Alamos National Laboratory; Mac Arthur, Duncan W [Los Alamos National Laboratory; Kondratov, Sergey [VNIIEF; Livke, Alexander [VNIIEF; Razinkov, Sergey [VNIIEF

    2010-01-01

    An attribute measurement system (AMS) measures a number of unclassified attributes of potentially classified material. By only displaying these unclassified results as red or green lights, the AMS protects potentially classified information while still generating confidence in the measurement result. The AVNG implementation that we describe is an AMS built by RFNC - VNIIEF in Sarov, Russia. To provide additional confidence, the AVNG was designed with two modes of operation. In the secure mode, potentially classified measurements can be made with only the simple red light/green light display. In the open mode, known unclassified material can be measured with complete display of the information collected from the radiation detectors. The AVNG demonstration, which occurred in Sarov, Russia in June 2009 for a joint US/Russian audience, included exercising both modes of AVNG operation using a number of multi-kg plutonium sources. In addition to describing the demonstration, we will show photographs and/or video taken of AVNG operation.

  19. Remote monitoring demonstration

    International Nuclear Information System (INIS)

    Caskey, Susan; Olsen, John

    2006-01-01

    The recently upgraded remote monitoring system at the Joyo Experimental Reactor uses a DCM-14 camera module and GEMINI software. The final data is compatible both with the IAEA-approved GARS review software and the ALIS software that was used for this demonstration. Features of the remote monitoring upgrade emphasized compatibility with IAEA practice. This presentation gives particular attention to the selection process for meeting network security considerations at the O'arai site. The Joyo system is different from the NNCA's ACPF system, in that it emphasizes use of IAEA standard camera technology and data acquisition and transmission software. In the demonstration itself, a temporary virtual private network (VPN) between the meeting room and the server at Sandia in Albuquerque allowed attendees to observe data stored from routine transmissions from the Joyo Fresh Fuel Storage to Sandia. Image files from a fuel movement earlier in the month showed Joyo workers and IAEA inspectors carrying out a transfer. (author)

  20. Commercial incineration demonstration

    International Nuclear Information System (INIS)

    Borduin, L.C.; Neuls, A.S.

    1981-01-01

    Low-level radioactive wastes (LLW) generated by nuclear utilities presently are shipped to commercial burial grounds for disposal. Substantially increasing shipping and disposal charges have sparked renewed industry interest in incineration and other advanced volume reduction techniques as potential cost-saving measures. Repeated inquiries from industry sources regarding LLW applicability of the Los Alamos controlled-air incineration (CAI) design led DOE to initiate this commercial demonstration program in FY-1980. The selected program approach to achieving CAI demonstration at a utility site is a DOE sponsored joint effort involving Los Alamos, a nuclear utility, and a liaison subcontractor. Required development tasks and responsibilities of the particpants are described. Target date for project completion is the end of FY-1985

  1. Automatic lighting controls demonstration

    Energy Technology Data Exchange (ETDEWEB)

    Rubinstein, F.; Verderber, R.

    1990-03-01

    The purpose of this work was to demonstrate, in a real building situation, the energy and peak demand reduction capabilities of an electronically ballasted lighting control system that can utilize all types of control strategies to efficiently manage lighting. The project has demonstrated that a state-of-the-art electronically ballasted dimmable lighting system can reduce energy and lighting demand by as least 50% using various combinations of control strategies. By reducing light levels over circulation areas (tuning) and reducing after hours light levels to accommodate the less stringent lighting demands of the cleaning crew (scheduling), lighting energy consumption on weekdays was reduced an average of 54% relative to the initial condition. 10 refs., 14 figs., 3 tabs.

  2. IGCC technology and demonstration

    Energy Technology Data Exchange (ETDEWEB)

    Palonen, J. [A. Ahlstrom Corporation, Karhula (Finland). Hans Ahlstrom Lab.; Lundqvist, R.G. [A. Ahlstrom Corporation, Helsinki (Finland); Staahl, K. [Sydkraft AB, Malmoe (Sweden)

    1996-12-31

    Future energy production will be performed by advanced technologies that are more efficient, more environmentally friendly and less expensive than current technologies. Integrated gasification combined cycle (IGCC) power plants have been proposed as one of these systems. Utilising biofuels in future energy production will also be emphasised since this lowers substantially carbon dioxide emissions into the atmosphere due to the fact that biomass is a renewable form of energy. Combining advanced technology and biomass utilisation is for this reason something that should and will be encouraged. A. Ahlstrom Corporation of Finland and Sydkraft AB of Sweden have as one part of company strategies adopted this approach for the future. The companies have joined their resources in developing a biomass-based IGCC system with the gasification part based on pressurised circulating fluidized-bed technology. With this kind of technology electrical efficiency can be substantially increased compared to conventional power plants. As a first concrete step, a decision has been made to build a demonstration plant. This plant, located in Vaernamo, Sweden, has already been built and is now in commissioning and demonstration stage. The system comprises a fuel drying plant, a pressurised CFB gasifier with gas cooling and cleaning, a gas turbine, a waste heat recovery unit and a steam turbine. The plant is the first in the world where the integration of a pressurised gasifier with a gas turbine will be realised utilising a low calorific gas produced from biomass. The capacity of the Vaernamo plant is 6 MW of electricity and 9 MW of district heating. Technology development is in progress for design of plants of sizes from 20 to 120 MWe. The paper describes the Bioflow IGCC system, the Vaernamo demonstration plant and experiences from the commissioning and demonstration stages. (orig.)

  3. The Majorana Demonstrator

    Energy Technology Data Exchange (ETDEWEB)

    Aguayo, Estanislao; Fast, James E.; Hoppe, Eric W.; Keillor, Martin E.; Kephart, Jeremy D.; Kouzes, Richard T.; LaFerriere, Brian D.; Merriman, Jason H.; Orrell, John L.; Overman, Nicole R.; Avignone, Frank T.; Back, Henning O.; Combs, Dustin C.; Leviner, L.; Young, A.; Barabash, Alexander S.; Konovalov, S.; Vanyushin, I.; Yumatov, Vladimir; Bergevin, M.; Chan, Yuen-Dat; Detwiler, Jason A.; Loach, J. C.; Martin, R. D.; Poon, Alan; Prior, Gersende; Vetter, Kai; Bertrand, F.; Cooper, R. J.; Radford, D. C.; Varner, R. L.; Yu, Chang-Hong; Boswell, M.; Elliott, S.; Gehman, Victor M.; Hime, Andrew; Kidd, M. F.; LaRoque, B. H.; Rielage, Keith; Ronquest, M. C.; Steele, David; Brudanin, V.; Egorov, Viatcheslav; Gusey, K.; Kochetov, Oleg; Shirchenko, M.; Timkin, V.; Yakushev, E.; Busch, Matthew; Esterline, James H.; Tornow, Werner; Christofferson, Cabot-Ann; Horton, Mark; Howard, S.; Sobolev, V.; Collar, J. I.; Fields, N.; Creswick, R.; Doe, Peter J.; Johnson, R. A.; Knecht, A.; Leon, Jonathan D.; Marino, Michael G.; Miller, M. L.; Robertson, R. G. H.; Schubert, Alexis G.; Wolfe, B. A.; Efremenko, Yuri; Ejiri, H.; Hazama, R.; Nomachi, Masaharu; Shima, T.; Finnerty, P.; Fraenkle, Florian; Giovanetti, G. K.; Green, M.; Henning, Reyco; Howe, M. A.; MacMullin, S.; Phillips, D.; Snavely, Kyle J.; Strain, J.; Vorren, Kris R.; Guiseppe, Vincente; Keller, C.; Mei, Dong-Ming; Perumpilly, Gopakumar; Thomas, K.; Zhang, C.; Hallin, A. L.; Keeter, K.; Mizouni, Leila; Wilkerson, J. F.

    2011-09-03

    A brief review of the history and neutrino physics of double beta decay is given. A description of the MAJORANA DEMONSTRATOR research and development program, including background reduction techniques, is presented in some detail. The application of point contact (PC) detectors to the experiment is discussed, including the effectiveness of pulse shape analysis. The predicted sensitivity of a PC detector array enriched to 86% to 76Ge is given.

  4. SAMSON Technology Demonstrator

    Science.gov (United States)

    2014-06-01

    escrow service in the operational environment. For the SAMSON TD, two key escrow systems were demonstrated: StrongAuth SKLES; a 3rd party key... escrow appliance; and A custom database-based key escrow system created for the SAMSON TD. The external label that is placed on file objects that...the key that was used to protect the file. When a SAMSON component presents a token to the KMS, the associated key is retrieved from the escrow and

  5. Learning From Demonstration?

    DEFF Research Database (Denmark)

    Koch, Christian; Bertelsen, Niels Haldor

    2014-01-01

    Demonstration projects are often used in the building sector to provide a basis for using new processes and/or products. The climate change agenda implies that construction is not only required to deliver value for the customer, cost reductions and efficiency but also sustainable buildings. This ...... prevailed over realizing a good indoor climate, which included sufficient heating. Project management must be able to handle quantitative complexity where simple issues add up to major challenges.......Demonstration projects are often used in the building sector to provide a basis for using new processes and/or products. The climate change agenda implies that construction is not only required to deliver value for the customer, cost reductions and efficiency but also sustainable buildings....... This paper reports on an early demonstration project, the Building of a passive house dormitory in the Central Region of Denmark in 2006-2009. The project was supposed to deliver value, lean design, prefabrication, quality in sustainability, certification according to German standards for passive houses...

  6. Buried Waste Integrated Demonstration Plan

    International Nuclear Information System (INIS)

    Kostelnik, K.M.

    1991-12-01

    This document presents the plan of activities for the Buried Waste Integrated Demonstration (BWID) program which supports the environmental restoration (ER) objectives of the Department of Energy (DOE) Complex. Discussed in this plan are the objectives, organization, roles and responsibilities, and the process for implementing and managing BWID. BWID is hosted at the Idaho National Engineering Laboratory (INEL), but involves participants from throughout the DOE Complex, private industry, universities, and the international community. These participants will support, demonstrate, and evaluate a suite of advanced technologies representing a comprehensive remediation system for the effective and efficient remediation of buried waste. The processes for identifying technological needs, screening candidate technologies for applicability and maturity, selecting appropriate technologies for demonstration, field demonstrating, evaluation of results and transferring technologies to environmental restoration programs are also presented. This document further describes the elements of project planning and control that apply to BWID. It addresses the management processes, operating procedures, programmatic and technical objectives, and schedules. Key functions in support of each demonstration such as regulatory coordination, safety analyses, risk evaluations, facility requirements, and data management are presented

  7. NAVAJO ELECTRIFICATION DEMONSTRATION PROJECT

    Energy Technology Data Exchange (ETDEWEB)

    Terry W. Battiest

    2008-06-11

    The Navajo Electrification Demonstration Project (NEDP) is a multi-year project which addresses the electricity needs of the unserved and underserved Navajo Nation, the largest American Indian tribe in the United States. The program serves to cumulatively provide off-grid electricty for families living away from the electricty infrastructure, line extensions for unserved families living nearby (less than 1/2 mile away from) the electricity, and, under the current project called NEDP-4, the construction of a substation to increase the capacity and improve the quality of service into the central core region of the Navajo Nation.

  8. Demonstration tokamak power plant

    Energy Technology Data Exchange (ETDEWEB)

    Abdou, M.; Baker, C.; Brooks, J.; Ehst, D.; Mattas, R.; Smith, D.L.; DeFreece, D.; Morgan, G.D.; Trachsel, C.

    1983-01-01

    A conceptual design for a tokamak demonstration power plant (DEMO) was developed. A large part of the study focused on examining the key issues and identifying the R and D needs for: (1) current drive for steady-state operation, (2) impurity control and exhaust, (3) tritium breeding blanket, and (4) reactor configuration and maintenance. Impurity control and exhaust will not be covered in this paper but is discussed in another paper in these proceedings, entitled Key Issues of FED/INTOR Impurity Control System.

  9. Demonstration of HITEX

    International Nuclear Information System (INIS)

    Morrison, H.D.; Woodall, K.B.

    1993-01-01

    A model reactor for HITEX successfully demonstrated the concept of high-temperature isotopic exchange in a closed loop simulating the conditions for fusion fuel cleanup. The catalyst of platinum on alumina pellets provided a surface area large enough to operate the reactor at 400 degrees celsius with flow rates up to 2 L/min. A 15-L tank containing a mixture of 4% CD 4 in H 2 was depleted in deuterium within 75 minutes down to 100 ppm HD above the natural concentration of HD in the make-up hydrogen stream. The application to tritium removal from tritiated impurities in a hydrogen stream will work as well or better

  10. Vortex Apparatus and Demonstrations

    Science.gov (United States)

    Shakerin, Said

    2010-05-01

    Vortex flow, from millimeter to kilometer in scale, is important in many scientific and technological areas. Examples are seen in water strider locomotion, from industrial pipe flow (wastewater treatment) to air traffic control (safe distance between aircrafts on a runway ready for takeoff) to atmospheric studies.2-5 In this paper, we focus on a particular vortex known as bathtub vortex (BTV). It occurs when water is drained from a hole at the bottom of a container such as a bathtub or a sink under the action of gravity. The vortex has a funnel shape with a central air core, resembling a tornado. We have designed a portable apparatus to demonstrate bathtub vortex on a continual basis. The apparatus consists of a clear cylinder supported by a frame over a water reservoir and a submersible pump. Young and old have been equally amazed by watching the demonstrations at various public presentations held at the University of the Pacific recently. With material cost of less than 100, the apparatus can be easily fabricated and used at other universities. With a short set-up time, it is an ideal device for promoting science to the general public, and it can be used to enhance lectures in physics courses as well.

  11. The IBL BOC Demonstrator

    CERN Document Server

    Ancu, J; The ATLAS collaboration; Falchieri, D; Flick, T; Gabrielli, A; Grosse-Knetter, J; Heim, T; Joseph, J; Krieger, N; Kugel, A; Morettini, P; Neumann, M; Polini, A; Schneider, B; Schroer, N

    2011-01-01

    The IBL is a new pixel detector layer to be installed at the ATLAS experiment at the LHC, CERN in 2013. It will be integrated into the general pixel readout and software framework, hence the off-detector readout electronics has to support the new front-end electronics whilst maintaining a high degree of interoperability to the components of the existing system. The off-detector readout is realised using a number of VME card pairs – ROD and BOC – plus a VME crate controller and a custom timing distribution system. The main elements of the new BOC design comprise optical interfaces towards the detector, signal conditioning and data recovery logic. We present the demonstrator used to verify the design approach. The demonstrator is based on a XILINX SP605 FPGA evaluation board and uses a Microblaze processor inside the FPGA to provide easy and flexible access to all essential BOC functions and the corresponding emulator modules, which enable full test of the entire BOC functionality even without any external ...

  12. Smart Grid Demonstration Project

    Energy Technology Data Exchange (ETDEWEB)

    Miller, Craig [National Rural Electric Cooperative Association, Arlington, VA (United States); Carroll, Paul [National Rural Electric Cooperative Association, Arlington, VA (United States); Bell, Abigail [National Rural Electric Cooperative Association, Arlington, VA (United States)

    2015-03-11

    The National Rural Electric Cooperative Association (NRECA) organized the NRECA-U.S. Department of Energy (DOE) Smart Grid Demonstration Project (DE-OE0000222) to install and study a broad range of advanced smart grid technologies in a demonstration that spanned 23 electric cooperatives in 12 states. More than 205,444 pieces of electronic equipment and more than 100,000 minor items (bracket, labels, mounting hardware, fiber optic cable, etc.) were installed to upgrade and enhance the efficiency, reliability, and resiliency of the power networks at the participating co-ops. The objective of this project was to build a path for other electric utilities, and particularly electrical cooperatives, to adopt emerging smart grid technology when it can improve utility operations, thus advancing the co-ops’ familiarity and comfort with such technology. Specifically, the project executed multiple subprojects employing a range of emerging smart grid technologies to test their cost-effectiveness and, where the technology demonstrated value, provided case studies that will enable other electric utilities—particularly electric cooperatives— to use these technologies. NRECA structured the project according to the following three areas: Demonstration of smart grid technology; Advancement of standards to enable the interoperability of components; and Improvement of grid cyber security. We termed these three areas Technology Deployment Study, Interoperability, and Cyber Security. Although the deployment of technology and studying the demonstration projects at coops accounted for the largest portion of the project budget by far, we see our accomplishments in each of the areas as critical to advancing the smart grid. All project deliverables have been published. Technology Deployment Study: The deliverable was a set of 11 single-topic technical reports in areas related to the listed technologies. Each of these reports has already been submitted to DOE, distributed to co-ops, and

  13. Fuel Cell Demonstration Program

    Energy Technology Data Exchange (ETDEWEB)

    Gerald Brun

    2006-09-15

    In an effort to promote clean energy projects and aid in the commercialization of new fuel cell technologies the Long Island Power Authority (LIPA) initiated a Fuel Cell Demonstration Program in 1999 with six month deployments of Proton Exchange Membrane (PEM) non-commercial Beta model systems at partnering sites throughout Long Island. These projects facilitated significant developments in the technology, providing operating experience that allowed the manufacturer to produce fuel cells that were half the size of the Beta units and suitable for outdoor installations. In 2001, LIPA embarked on a large-scale effort to identify and develop measures that could improve the reliability and performance of future fuel cell technologies for electric utility applications and the concept to establish a fuel cell farm (Farm) of 75 units was developed. By the end of October of 2001, 75 Lorax 2.0 fuel cells had been installed at the West Babylon substation on Long Island, making it the first fuel cell demonstration of its kind and size anywhere in the world at the time. Designed to help LIPA study the feasibility of using fuel cells to operate in parallel with LIPA's electric grid system, the Farm operated 120 fuel cells over its lifetime of over 3 years including 3 generations of Plug Power fuel cells (Lorax 2.0, Lorax 3.0, Lorax 4.5). Of these 120 fuel cells, 20 Lorax 3.0 units operated under this Award from June 2002 to September 2004. In parallel with the operation of the Farm, LIPA recruited government and commercial/industrial customers to demonstrate fuel cells as on-site distributed generation. From December 2002 to February 2005, 17 fuel cells were tested and monitored at various customer sites throughout Long Island. The 37 fuel cells operated under this Award produced a total of 712,635 kWh. As fuel cell technology became more mature, performance improvements included a 1% increase in system efficiency. Including equipment, design, fuel, maintenance

  14. Fusion Power Demonstration III

    International Nuclear Information System (INIS)

    Lee, J.D.

    1985-07-01

    This is the third in the series of reports covering the Fusion Power Demonstration (FPD) design study. This volume considers the FPD-III configuration that incorporates an octopole end plug. As compared with the quadrupole end-plugged designs of FPD-I and FPD-II, this octopole configuration reduces the number of end cell magnets and shortens the minimum ignition length of the central cell. The end-cell plasma length is also reduced, which in turn reduces the size and cost of the end cell magnets and shielding. As a contiuation in the series of documents covering the FPD, this report does not stand alone as a design description of FPD-III. Design details of FPD-III subsystems that do not differ significantly from those of the FPD-II configuration are not duplicated in this report

  15. Jennings Demonstration PLant

    Energy Technology Data Exchange (ETDEWEB)

    Russ Heissner

    2010-08-31

    Verenium operated a demonstration plant with a capacity to produce 1.4 million gallons of cellulosic ethanol from agricultural resiues for about two years. During this time, the plant was able to evaluate the technical issues in producing ethanol from three different cellulosic feedstocks, sugar cane bagasse, energy cane, and sorghum. The project was intended to develop a better understanding of the operating parameters that would inform a commercial sized operation. Issues related to feedstock variability, use of hydrolytic enzymes, and the viability of fermentative organisms were evaluated. Considerable success was achieved with pretreatment processes and use of enzymes but challenges were encountered with feedstock variability and fermentation systems. Limited amounts of cellulosic ethanol were produced.

  16. Fusion Power Demonstration III

    Energy Technology Data Exchange (ETDEWEB)

    Lee, J.D. (ed.)

    1985-07-01

    This is the third in the series of reports covering the Fusion Power Demonstration (FPD) design study. This volume considers the FPD-III configuration that incorporates an octopole end plug. As compared with the quadrupole end-plugged designs of FPD-I and FPD-II, this octopole configuration reduces the number of end cell magnets and shortens the minimum ignition length of the central cell. The end-cell plasma length is also reduced, which in turn reduces the size and cost of the end cell magnets and shielding. As a contiuation in the series of documents covering the FPD, this report does not stand alone as a design description of FPD-III. Design details of FPD-III subsystems that do not differ significantly from those of the FPD-II configuration are not duplicated in this report.

  17. Some Pros and Cons of Laptop Use in Class

    Science.gov (United States)

    Harris, R. W.

    2018-05-01

    We did not have laptops or computer networks in schools in 1968, when I started teaching physics. When classroom computers became available, followed by the internet, I greeted them as great educational tools. I developed my own website in order to provide reference material and assignments for my students. I found that online assignments were more likely than traditional ones to be completed. I also had my own system making password-protected grades available online. The parents loved it. I began giving some tests online. However, there is a downside to laptop use in class.

  18. A demonstration of the uncertainty in predicting the estrogenic activity of individual chemicals and mixtures from an in vitro estrogen receptor transcriptional activation assay (T47D-KBluc) to the in vivo uterotrophic assay using oral exposure

    Science.gov (United States)

    In vitro estrogen receptor assays are valuable screening tools for identifying environmental samples and chemicals that display estrogenic activity. However, in vitro potency cannot necessarily be extrapolated to estimates of in vivo potency because in vitro assays are currently...

  19. The buried waste integrated demonstration

    International Nuclear Information System (INIS)

    Kostelnik, K.M.

    1991-01-01

    There are numerous locations throughout the Department of Energy (DOE) Complex where wastes have been buried in the ground or stored for future disposal. Much of this buried waste is contaminated with hazardous and radioactive materials. An extensive research program has been initiated at the Idaho National Engineering Laboratory (INEL) to develop and demonstrate advanced remediation techniques for DOE Complex buried waste. The purpose of the Buried Waste Integrated Demonstration (BWID), is to develop a scientifically sound and deployable remediation system consisting of advanced technologies which address the buried waste characteristics of the DOE Complex. This comprehensive remediation system win include technologies for the entire remediation cycle (cradle-to-grave). Technologies developed and demonstrated within the BWID will be transferred to the DOE Complex sites with buried waste, to private industry, and to universities. Multidirectional technology transfer is encouraged by the BWID. Identification and evaluation of plausible technological solutions are an ongoing activity of the BWID. A number of technologies are currently under development throughout the DOE Complex, private industry, and universities. Technology integration mechanisms have been established by BWID to facilitate collaborative research and demonstration of applicable remedial technologies for buried waste. Successful completion of the BWID will result in the development of a proven and deployable system at the INEL and other DOE Complex buried waste sites, thereby supporting the DOE Complex's environmental restoration objectives

  20. A Demonstration of Lusail

    KAUST Repository

    Mansour, Essam

    2017-05-10

    There has been a proliferation of datasets available as interlinked RDF data accessible through SPARQL endpoints. This has led to the emergence of various applications in life science, distributed social networks, and Internet of Things that need to integrate data from multiple endpoints. We will demonstrate Lusail; a system that supports the need of emerging applications to access tens to hundreds of geo-distributed datasets. Lusail is a geo-distributed graph engine for querying linked RDF data. Lusail delivers outstanding performance using (i) a novel locality-aware query decomposition technique that minimizes the intermediate data to be accessed by the subqueries, and (ii) selectivityawareness and parallel query execution to reduce network latency and to increase parallelism. During the demo, the audience will be able to query actually deployed RDF endpoints as well as large synthetic and real benchmarks that we have deployed in the public cloud. The demo will also show that Lusail outperforms state-of-the-art systems by orders of magnitude in terms of scalability and response time.

  1. Performance Demonstration Program Management Plan

    International Nuclear Information System (INIS)

    2005-01-01

    To demonstrate compliance with the Waste Isolation Pilot Plant (WIPP) waste characterization program, each testing and analytical facility performing waste characterization activities participates in the Performance Demonstration Program (PDP). The PDP serves as a quality control check against expected results and provides information about the quality of data generated in the characterization of waste destined for WIPP. Single blind audit samples are prepared and distributed by an independent organization to each of the facilities participating in the PDP. There are three elements within the PDP: analysis of simulated headspace gases, analysis of solids for Resource Conservation and Recovery Act (RCRA) constituents, and analysis for transuranic (TRU) radionuclides using nondestructive assay (NDA) techniques. Because the analysis for TRU radionuclides using NDA techniques involves both the counting of drums and standard waste boxes, four PDP plans are required to describe the activities of the three PDP elements. In accordance with these PDP plans, the reviewing and approving authority for PDP results and for the overall program is the CBFO PDP Appointee. The CBFO PDP Appointee is responsible for ensuring the implementation of each of these plans by concurring with the designation of the Program Coordinator and by providing technical oversight and coordination for the program. The Program Coordinator will designate the PDP Manager, who will coordinate the three elements of the PDP. The purpose of this management plan is to identify how the requirements applicable to the PDP are implemented during the management and coordination of PDP activities. The other participants in the program (organizations that perform site implementation and activities under CBFO contracts or interoffice work orders) are not covered under this management plan. Those activities are governed by the organization's quality assurance (QA) program and procedures or as otherwise directed by CBFO.

  2. Control of Gene Expression in Leptospira spp. by Transcription Activator-Like Effectors Demonstrates a Potential Role for LigA and LigB in Leptospira interrogans Virulence.

    Science.gov (United States)

    Pappas, Christopher J; Picardeau, Mathieu

    2015-11-01

    Leptospirosis is a zoonotic disease that affects ∼1 million people annually, with a mortality rate of >10%. Currently, there is an absence of effective genetic manipulation tools for targeted mutagenesis in pathogenic leptospires. Transcription activator-like effectors (TALEs) are a recently described group of repressors that modify transcriptional activity in prokaryotic and eukaryotic cells by directly binding to a targeted sequence within the host genome. To determine the applicability of TALEs within Leptospira spp., two TALE constructs were designed. First, a constitutively expressed TALE gene specific for the lacO-like region upstream of bgaL was trans inserted in the saprophyte Leptospira biflexa (the TALEβgal strain). Reverse transcriptase PCR (RT-PCR) analysis and enzymatic assays demonstrated that BgaL was not expressed in the TALEβgal strain. Second, to study the role of LigA and LigB in pathogenesis, a constitutively expressed TALE gene with specificity for the homologous promoter regions of ligA and ligB was cis inserted into the pathogen Leptospira interrogans (TALElig). LigA and LigB expression was studied by using three independent clones: TALElig1, TALElig2, and TALElig3. Immunoblot analysis of osmotically induced TALElig clones demonstrated 2- to 9-fold reductions in the expression levels of LigA and LigB, with the highest reductions being noted for TALElig1 and TALElig2, which were avirulent in vivo and nonrecoverable from animal tissues. This study reconfirms galactosidase activity in the saprophyte and suggests a role for LigA and LigB in pathogenesis. Collectively, this study demonstrates that TALEs are effective at reducing the expression of targeted genes within saprophytic and pathogenic strains of Leptospira spp., providing an additional genetic manipulation tool for this genus. Copyright © 2015, American Society for Microbiology. All Rights Reserved.

  3. Shortened telomere length is demonstrated in T-cell subsets together with a pronounced increased telomerase activity in CD4 positive T cells from blood of patients with mycosis fungoides and parapsoriasis.

    Science.gov (United States)

    Wu, K D; Hansen, E R

    2001-10-01

    We have recently demonstrated that telomerase activity is increased and telomere length shortened in lymphocytes from peripheral blood of patients with cutaneous T-cell lymphoma. In order to determine which cell type has increased telomerase activity and shortened telomere length, CD4+, CD8+, CLA+ CD3+ and CLA- CD3+ T cells were isolated from peripheral blood of 25 patients, including 15 patients with mycosis fungoides and 10 patients with parapsoriasis. Eleven healthy individuals were used as controls; CD19+ B cells were separated from each individual as an internal control. The results showed that the increased telomerase activity was significantly predominating in the CD4+ T-cell subset. Significantly shortened telomere length was found in CD4+ and CD8+ T-cell subsets from the patients compared with the same cell subsets obtained from healthy individuals. However, no difference was observed between the subsets; CD19+ B cells collected from patients and healthy control individuals had similar telomerase activity and telomere length which was significantly different from the values found in T cells. The telomere length was significantly shorter in CLA+ CD3+ subset than in CLA- CD3+ subset. Interestingly, increased telomerase activity and shortened telomere length was also detected in CD4+ T cells from patients with parapsoriasis indicating that alteration of telomerase activity and telomere length in CD4+ T cells is an early event in the pathogenesis of cutaneous T-cell lymphoma. Thus, the results indicate that a significant high level of telomerase activity and shortened telomere length frequently occur in T cells of patients with CTCL and may reflect tumorigenesis.

  4. LIMB Demonstration Project Extension and Coolside Demonstration. [Final report

    Energy Technology Data Exchange (ETDEWEB)

    Goots, T.R.; DePero, M.J.; Nolan, P.S.

    1992-11-10

    This report presents results from the limestone Injection Multistage Burner (LIMB) Demonstration Project Extension. LIMB is a furnace sorbent injection technology designed for the reduction of sulfur dioxide (SO{sub 2}) and nitrogen oxides (NO{sub x}) emissions from coal-fired utility boilers. The testing was conducted on the 105 Mwe, coal-fired, Unit 4 boiler at Ohio Edison`s Edgewater Station in Lorain, Ohio. In addition to the LIMB Extension activities, the overall project included demonstration of the Coolside process for S0{sub 2} removal for which a separate report has been issued. The primary purpose of the DOE LIMB Extension testing, was to demonstrate the generic applicability of LIMB technology. The program sought to characterize the S0{sub 2} emissions that result when various calcium-based sorbents are injected into the furnace, while burning coals having sulfur content ranging from 1.6 to 3.8 weight percent. The four sorbents used included calcitic limestone, dolomitic hydrated lime, calcitic hydrated lime, and calcitic hydrated lime with a small amount of added calcium lignosulfonate. The results include those obtained for the various coal/sorbent combinations and the effects of the LIMB process on boiler and plant operations.

  5. Benchmarking road safety performance: Identifying a meaningful reference (best-in-class).

    Science.gov (United States)

    Chen, Faan; Wu, Jiaorong; Chen, Xiaohong; Wang, Jianjun; Wang, Di

    2016-01-01

    For road safety improvement, comparing and benchmarking performance are widely advocated as the emerging and preferred approaches. However, there is currently no universally agreed upon approach for the process of road safety benchmarking, and performing the practice successfully is by no means easy. This is especially true for the two core activities of which: (1) developing a set of road safety performance indicators (SPIs) and combining them into a composite index; and (2) identifying a meaningful reference (best-in-class), one which has already obtained outstanding road safety practices. To this end, a scientific technique that can combine the multi-dimensional safety performance indicators (SPIs) into an overall index, and subsequently can identify the 'best-in-class' is urgently required. In this paper, the Entropy-embedded RSR (Rank-sum ratio), an innovative, scientific and systematic methodology is investigated with the aim of conducting the above two core tasks in an integrative and concise procedure, more specifically in a 'one-stop' way. Using a combination of results from other methods (e.g. the SUNflower approach) and other measures (e.g. Human Development Index) as a relevant reference, a given set of European countries are robustly ranked and grouped into several classes based on the composite Road Safety Index. Within each class the 'best-in-class' is then identified. By benchmarking road safety performance, the results serve to promote best practice, encourage the adoption of successful road safety strategies and measures and, more importantly, inspire the kind of political leadership needed to create a road transport system that maximizes safety. Copyright © 2015 Elsevier Ltd. All rights reserved.

  6. Learning Strategy in Class Management: A Reflection from Manado Case

    Science.gov (United States)

    Suardi Wekke, Ismail; Yandra, Alexander; Hamuddin, Budianto

    2017-12-01

    This article is a research conducted with qualitative approach with various case studies underlining a strategy that becomes the basis for classroom management. The article discusses and links to the learning revolution that becomes today’s demands, including a discussion that analyses the condition of learners. The article based its data preliminary study conducted in Manado in the province of North Sulawesi in Indonesia. This region has its own characteristics with the encounter of Muslims and the Protestant community for century. Due to its uniqueness 3 Moslem schools and 3 Protestant schools in Manado were selected to study. Data collection was conducted for a year, from May 2016 to April 2017. The study employ four stages research steps: identification, data collection, data validity checking, and directed discussion. The stages include observation and in-depth interviews and conducting focus group discussions. Two important thought about the essence of learning strategy and learning revolution in class were shared briefly within this article.

  7. Demonstration of cost effective low-energy buildings. Final report of the project 'Danish activities in the EU-CONCERTO project CLASS 1 in Stenloese'; Demonstration af omkostningseffektive lavenergibygninger. Afslutningsrapport fra EFP 2007 projektet 'Dansk indsats i EU-CONCERTO projektet CLASS 1 i Stenloese Syd'

    Energy Technology Data Exchange (ETDEWEB)

    Moerck, O. (Cenergia Energy Consultants, Herlev (Denmark)); Engelund Thomsen, K.; Rose, J. (SBi, Aalborg (Denmark)); Dates Joergensen, M. (Egedal Kommune, Stenloese (Denmark))

    2011-01-15

    The municipality of Egedal decided in 2006 to strengthen the energy requirements for a new settlement to be erected in the municipality. During the years 2007-2011 a total of 442 dwellings was to be designed and constructed with a heating demand corresponding to the new Danish low-energy standard referred to as 'low-energy class 1' in a new settlement called Stenloese Syd. 65 dwellings were to be designed and constructed with a yearly heating demand of 15 kWh/m{sup 2}. Furthermore the Concerto community includes a kindergarten and an activity centre for elderly people. After 3 years the kindergarten, the centre for elderly people, 65 dense lowrise housing units and 30 single family houses have been constructed. In parallel 15 deliverables/reports have been produced covering a number of different aspects: User preferences, proactive planning processes, product development, design guidelines, ecolabelling and training activities. A comparison was made of the calculated and measured energy consumption in a single-family house and in the KAB social housing project (65 dwellings). In the coming months the next demonstration buildings are to be completed, the extended district heating network (Plan B) including a woodchip furnace and a large array of solar collectors is to be implemented and the monitoring continued. The users will be followed and assisted and the lessons learned will be documented. It is expected that the experiences, lessons learned and R and D carried out as part of the Class 1 project will pave the way for the development, design and construction of sustainable, low or zero CO2-emission communities in the future. Further information about the Class1 project is to be found on the project website: www.class1.dk. (Author)

  8. Favorite Demonstrations: Gaseous Diffusion: A Demonstration of Graham's Law.

    Science.gov (United States)

    Kauffman, George B.; Ebner, Ronald D.

    1985-01-01

    Describes a demonstration in which gaseous ammonia and hydrochloric acid are used to illustrate rates of diffusion (Graham's Law). Simple equipment needed for the demonstration include a long tube, rubber stoppes, and cotton. Two related demonstrations are also explained. (DH)

  9. Performance analysis of commercial MOSFET packages in Class E converter operating at 2.56 MHz

    DEFF Research Database (Denmark)

    Nair, Unnikrishnan Raveendran; Munk-Nielsen, Stig; Jørgensen, Asger Bjørn

    2017-01-01

    are not commercially available and power modules have to be custom designed for these applications. This work demonstrates performance of various commercial MOSFET packages at frequency of 2.56 MHz. Commercial SiC MOSFETs in TO-247 and D2Pak packs are tested in Class E resonant converter operating at 2.56 MHz...... resistance and high temperature operation over Si devices have aided in the paradigm shift towards wide bandgap devices. The low gate charge requirements of SiC MOSFETs enables use of these devices in radio frequency (RF) converters using resonant topologies operating at MHz frequency range. The RF...... converters employed in various industrial applications are currently realized with vacuum tubes. Replacing vacuum tubes with solid state devices provides greater reliability. This requires power switches transferring high power at high switching speeds. Wide bandgap devices operating at these specifications...

  10. Performance analysis of commercial MOSFET packages in Class E converter operating at 2.56 MHz

    DEFF Research Database (Denmark)

    Nair, Unnikrishnan Raveendran; Munk-Nielsen, Stig; Jørgensen, Asger Bjørn

    2017-01-01

    resistance and high temperature operation over Si devices have aided in the paradigm shift towards wide bandgap devices. The low gate charge requirements of SiC MOSFETs enables use of these devices in radio frequency (RF) converters using resonant topologies operating at MHz frequency range. The RF...... converters employed in various industrial applications are currently realized with vacuum tubes. Replacing vacuum tubes with solid state devices provides greater reliability. This requires power switches transferring high power at high switching speeds. Wide bandgap devices operating at these specifications...... are not commercially available and power modules have to be custom designed for these applications. This work demonstrates performance of various commercial MOSFET packages at frequency of 2.56 MHz. Commercial SiC MOSFETs in TO-247 and D2Pak packs are tested in Class E resonant converter operating at 2.56 MHz...

  11. Aerospace Communications Security Technologies Demonstrated

    Science.gov (United States)

    Griner, James H.; Martzaklis, Konstantinos S.

    2003-01-01

    In light of the events of September 11, 2001, NASA senior management requested an investigation of technologies and concepts to enhance aviation security. The investigation was to focus on near-term technologies that could be demonstrated within 90 days and implemented in less than 2 years. In response to this request, an internal NASA Glenn Research Center Communications, Navigation, and Surveillance Aviation Security Tiger Team was assembled. The 2-year plan developed by the team included an investigation of multiple aviation security concepts, multiple aircraft platforms, and extensively leveraged datalink communications technologies. It incorporated industry partners from NASA's Graphical Weather-in-the-Cockpit research, which is within NASA's Aviation Safety Program. Two concepts from the plan were selected for demonstration: remote "black box," and cockpit/cabin surveillance. The remote "black box" concept involves real-time downlinking of aircraft parameters for remote monitoring and archiving of aircraft data, which would assure access to the data following the loss or inaccessibility of an aircraft. The cockpit/cabin surveillance concept involves remote audio and/or visual surveillance of cockpit and cabin activity, which would allow immediate response to any security breach and would serve as a possible deterrent to such breaches. The datalink selected for the demonstrations was VDL Mode 2 (VHF digital link), the first digital datalink for air-ground communications designed for aircraft use. VDL Mode 2 is beginning to be implemented through the deployment of ground stations and aircraft avionics installations, with the goal of being operational in 2 years. The first demonstration was performed December 3, 2001, onboard the LearJet 25 at Glenn. NASA worked with Honeywell, Inc., for the broadcast VDL Mode 2 datalink capability and with actual Boeing 757 aircraft data. This demonstration used a cockpitmounted camera for video surveillance and a coupling to

  12. A simple in-class experiment on the efficient production of the pure public good touching the median voter theorem

    Directory of Open Access Journals (Sweden)

    Domenico Raguseo

    2012-01-01

    Full Text Available The paper show possibilities of introducing modern methods into public economy and finance teaching. We present in-class experiment which illustrates one of typical model from political economy – the median voter theorem. Students are endowed with cards that represent their preferences on different levels of the public good production. They compare benefit they can get from different levels of public good (these levels represent three candidates and then they vote for the most preferred level. This process continues for several rounds, with a new election taking place with a different sample of students. The in-class experiment demonstrates that the simplicity of the median voter theorem, when there are more than two candidates, provides only an imprecise prediction of the collective choice.The methodology of the experiment is enriched by the statistical analysis of experimental results from experiment cartied out by the authors in Czech Republic and Slovakia.

  13. Exploration Medical System Demonstration Project

    Science.gov (United States)

    Chin, D. A.; McGrath, T. L.; Reyna, B.; Watkins, S. D.

    2011-01-01

    A near-Earth Asteroid (NEA) mission will present significant new challenges including hazards to crew health created by exploring a beyond low earth orbit destination, traversing the terrain of asteroid surfaces, and the effects of variable gravity environments. Limited communications with ground-based personnel for diagnosis and consultation of medical events require increased crew autonomy when diagnosing conditions, creating treatment plans, and executing procedures. Scope: The Exploration Medical System Demonstration (EMSD) project will be a test bed on the International Space Station (ISS) to show an end-to-end medical system assisting the Crew Medical Officers (CMO) in optimizing medical care delivery and medical data management during a mission. NEA medical care challenges include resource and resupply constraints limiting the extent to which medical conditions can be treated, inability to evacuate to Earth during many mission phases, and rendering of medical care by a non-clinician. The system demonstrates the integration of medical technologies and medical informatics tools for managing evidence and decision making. Project Objectives: The objectives of the EMSD project are to: a) Reduce and possibly eliminate the time required for a crewmember and ground personnel to manage medical data from one application to another. b) Demonstrate crewmember's ability to access medical data/information via a software solution to assist/aid in the treatment of a medical condition. c) Develop a common data management architecture that can be ubiquitously used to automate repetitive data collection, management, and communications tasks for all crew health and life sciences activities. d) Develop a common data management architecture that allows for scalability, extensibility, and interoperability of data sources and data users. e) Lower total cost of ownership for development and sustainment of peripheral hardware and software that use EMSD for data management f) Provide

  14. A randomised, double-blind, parallel-group study to demonstrate equivalence in efficacy and safety of CT-P13 compared with innovator infliximab when coadministered with methotrexate in patients with active rheumatoid arthritis: the PLANETRA study

    Science.gov (United States)

    Yoo, Dae Hyun; Hrycaj, Pawel; Miranda, Pedro; Ramiterre, Edgar; Piotrowski, Mariusz; Shevchuk, Sergii; Kovalenko, Volodymyr; Prodanovic, Nenad; Abello-Banfi, Mauricio; Gutierrez-Ureña, Sergio; Morales-Olazabal, Luis; Tee, Michael; Jimenez, Renato; Zamani, Omid; Lee, Sang Joon; Kim, HoUng; Park, Won; Müller-Ladner, Ulf

    2013-01-01

    Objectives To compare the efficacy and safety of innovator infliximab (INX) and CT-P13, an INX biosimilar, in active rheumatoid arthritis patients with inadequate response to methotrexate (MTX) treatment. Methods Phase III randomised, double-blind, multicentre, multinational, parallel-group study. Patients with active disease despite MTX (12.5–25 mg/week) were randomised to receive 3 mg/kg of CT-P13 (n=302) or INX (n=304) with MTX and folic acid. The primary endpoint was the American College of Rheumatology 20% (ACR20) response at week 30. Therapeutic equivalence of clinical response according to ACR20 criteria was concluded if the 95% CI for the treatment difference was within ±15%. Secondary endpoints included ACR response criteria, European League Against Rheumatism (EULAR) response criteria, change in Disease Activity Score 28 (DAS28), Medical Outcomes Study Short-Form Health Survey (SF-36), Simplified Disease Activity Index, Clinical Disease Activity Index, as well as pharmacokinetic (PK) and pharmacodynamic (PD) parameters, safety and immunogenicity. Results At week 30, ACR20 responses were 60.9% for CT-P13 and 58.6% for INX (95% CI −6% to 10%) in the intention-to-treat population. The proportions in CT-P13 and INX groups achieving good or moderate EULAR responses (C reactive protein (CRP)) at week 30 were 85.8% and 87.1%, respectively. Low disease activity or remission according to DAS28–CRP, ACR–EULAR remission rates, ACR50/ACR70 responses and all other PK and PD endpoints were highly similar at week 30. Incidence of drug-related adverse events (35.2% vs 35.9%) and detection of antidrug antibodies (48.4% vs 48.2%) were highly similar for CT-P13 and INX, respectively. Conclusions CT-P13 demonstrated equivalent efficacy to INX at week 30, with a comparable PK profile and immunogenicity. CT-P13 was well tolerated, with a safety profile comparable with that of INX. ClinicalTrials.gov Identifier NCT01217086 PMID:23687260

  15. Combining Chalk Talk with PowerPoint to Increase In-class Student Engagement

    Directory of Open Access Journals (Sweden)

    Swati Betharia

    2016-11-01

    Full Text Available In striving to attain a higher degree of in-class student engagement, and target a larger number of preferred student-learning styles, this case study describes a multimodal teaching approach. PowerPoint slides have gradually gained popularity over the more traditional chalk and talk lecture design. The student population in today’s age seeks more non-passive modes of information delivery. Numerous novel approaches to enhance active learning, such as flipped classroom and problem-based learning, have recently been explored. While working well for therapeutic and lab-based courses, these formats may not be best-suited for all basic science topics. The importance of basic science in a pharmacy curriculum is well emphasized in the 2016 ACPE Standards. To actively involve students in a pharmacology lecture on diuretics, a session was designed to combine the PowerPoint and chalk talk approaches. Students created 10 concept diagrams following an instructor, who explained each step in the process using a document camera. For visual learners, these diagrams provided a layered representation of the information, gradually increasing in complexity. For learners with a preference for the reading learning style, the information was also available in corresponding PowerPoint slides. Scores from pre- and post-session quizzes indicated a high level of concept understanding and recall (median 1 [IQR 0 – 2] vs 4 [IQR 3 – 5]; p<0.001. The student perception survey data reported higher in-class attention levels (76%, an appreciation for the utility of self-created concept diagrams (88%, and a call for additional sessions being presented in this format (73%. Targeting a variety of student learning styles by using the active development of concept diagrams, in addition to traditional PowerPoint slides, can promote student engagement and enhance content understanding.   Type: Case Study

  16. Codes & standards research, development & demonstration Roadmap

    Energy Technology Data Exchange (ETDEWEB)

    None, None

    2008-07-22

    This Roadmap is a guide to the Research, Development & Demonstration activities that will provide data required for SDOs to develop performance-based codes and standards for a commercial hydrogen fueled transportation sector in the U.S.

  17. Relative solubility of cations in Class F fly ash.

    Science.gov (United States)

    Kim, Ann G; Kazonich, George; Dahlberg, Michael

    2003-10-01

    Coal utilization byproducts (CUB), such as fly ash, contain cations that may be released during exposure to fluids such as acid rain or acid mine drainage. Researchers at the Department of Energy's National Energy Technology Laboratory (DOE/NETL) have conducted a long-term column leaching study of 32 Class F fly ash samples from pulverized coal (PC) combustion, and quantified the release of 19 cations in four leachants with a pH between 1.2 and 12. The relative solubility (M(L/T)) of each cation was defined as the total mass leached (M(L)) relative to the concentration (M(T)) of that element in the fly ash sample. A frequency distribution of relative solubility values was computed with ranges defined as insoluble, slightly soluble, moderately soluble, and very soluble. On the basis of this sample set, Ba, Cd, Fe, Pb, Sb, and Se in PC fly ash are insoluble. The elements Al, Be, Ca, Co, Cr, Cu, K, Mg, Mn, Na, Ni, and Zn are slightly to moderately acid soluble. Only Ca and Na are water soluble; As and Ca are soluble in the basic solution, The results of this study indicate that the extent to which cations in Class F PC fly ash can be leached by naturally occurring fluids is very limited.

  18. Information Integration Technology Demonstration (IITD)

    National Research Council Canada - National Science Library

    Loe, Richard

    2001-01-01

    The objectives of the Information Integration Technology Demonstration (IITD) were to investigate, design a software architecture and demonstrate a capability to display intelligence data from multiple disciplines...

  19. Challenging demonstrations in the physics classroom

    International Nuclear Information System (INIS)

    Raz, E.

    2004-01-01

    Full Text: We consider the role of classroom demonstrations in improving students understanding of physics lectures and suggest criteria to decide whether a given demonstration will be pedagogically useful. In the light of these considerations, we performed two series of related experiments before groups of high-school students. We shall perform one of them with active participation from the audience. We shall also show some challenging demonstrations performed in the final stages of the Israeli Physics Olympiad for high-school students

  20. The traditional medicine Spilanthes acmella, and the alkylamides spilanthol and undeca-2E-ene-8,10-diynoic acid isobutylamide, demonstrate in vitro and in vivo anti-malarial activity

    Science.gov (United States)

    Spelman, Kevin; Depoix, Delphine; McCray, Megan; Mouray, Elisabeth; Grellier, Philippe

    2010-01-01

    Spilanthes spp. are used as traditional herbal medicines in Africa and India to treat malaria. Yet, to date, there is no data on active constituents or most effective extraction methods for this indication. The isolated alkylamides, spilanthol and undeca-2E-ene-8,10-diynoic acid isobutylamide, found in S. acmella Murr., were shown to have IC50s of 16.5 μg/mL and 41.4 μg/mL on Plasmodium falciparum strain PFB and IC50s of 5.8 μg/mL and 16.3 μg/mL for the chloroquine resistant P. falciparum K1 strain, respectively. Further investigations revealed that at relatively low concentrations, spilanthol and the water extract of S. acmella reduced the parasitemia 59% and 53% in mice infected with P. yoelii yoelii 17XNL at 5 mg/kg and 50 mg/kg, respectively. Unexpectedly, the 95% ethanol extract of S. acmella was less effective (36% reduction in parasitemia) at 50 mg/kg. These results provide the first evidence supporting S. acmella against malaria and demonstrating active constituents in S. acmella against P. falciparum. PMID:22692989

  1. Microleakage of four composite resin systems in class II restorations.

    Science.gov (United States)

    Majeed, A; Osman, Y I; Al-Omari, T

    2009-11-01

    To compare the microleakage at the enamel and dentine/cementum margins of three nanocomposites and a microhybrid composite in Class II restorations. Four light-cured dental resin restorative materials in combination with their respective bonding agents were investigated. Eighty non-carious, extracted human molars were divided into 4 groups of 20 teeth each. The apices of the teeth were sealed with a resin modified glass ionomer cement. Standardized Class II slot cavities were prepared on the proximal surfaces of each tooth. Each group had an equal number of cavities with gingival margins on enamel and on dentine/cementum. Restorations were placed as indicated: Group 1 (G1): Ceram-X mono/Prime & Bond NT (Dentsply), G2: Premise/ OptiBond Solo Plus (Kerr), G3: Grandio/Admira Bond (VOCO), G4: Z100/Adper Scotchbond Multi-Purpose (3M ESPE). After thermocycling and immersion in 0.5% methylene blue dye solution, the teeth were sectioned and dye penetration was scored on a scale of 0 to 3 on both the enamel and dentine/cementum margins. The data were analyzed using a Kruskal-Wallis one way ANOVA and Mann-Whitney U test of ranks (significance at p Grandio/Admira Bond showed significantly lower microleakage when compared to the other materials tested while Z100/Adper Scotchbond Multi-Purpose showed the largest microleakage (p Grandio/Admira Bond showing the least microleakage when compared to the other three materials tested. At the enamel margins, all materials tested performed reasonably well.

  2. Cargo Data Management Demonstration System

    Science.gov (United States)

    1974-02-01

    Delays in receipt and creation of cargo documents are a problem in international trade. The work described demonstrates some of the advantages and capabilities of a computer-based cargo data management system. A demonstration system for data manageme...

  3. Integrated Ground Operations Demonstration Units

    Data.gov (United States)

    National Aeronautics and Space Administration — The overall goal of the AES Integrated Ground Operations Demonstration Units (IGODU) project is to demonstrate cost efficient cryogenic operations on a relevant...

  4. Teleoperation for learning by demonstration

    DEFF Research Database (Denmark)

    Kukliński, Kamil; Fischer, Kerstin; Marhenke, Ilka

    2014-01-01

    Learning by demonstration is a useful technique to augment a robot's behavioral inventory, and teleoperation allows lay users to demonstrate novel behaviors intuitively to the robot. In this paper, we compare two modes of teleoperation of an industrial robot, the demonstration by means of a data...... glove and by means of a control object (peg). Experiments with 16 lay users, performing assembly task on the Cranfield benchmark objects, show that the control peg leads to more success, more efficient demonstration and fewer errors....

  5. Helicopter detection and classification demonstrator

    NARCIS (Netherlands)

    Koersel, A.C. van

    2000-01-01

    A technology demonstrator that detects and classifies different helicopter types automatically, was developed at TNO-FEL. The demonstrator is based on a PC, which receives its acoustic input from an all-weather microphone. The demonstrator uses commercial off-the-shelf hardware to digitize the

  6. Using Laptop Computers in Class: A Student Motivation Perspective

    Science.gov (United States)

    Houle, Philip A.; Reed, Diana; Vaughan, Amy Grace; Clayton, Suzanne R.

    2013-01-01

    This study examined the reasons why students choose to take laptop computers into college classes. The model involved the individual student choice involving opportunity, ability and motivation. The resulting model demonstrated how some (primary) factors, such as effective learning, directly impact the laptop usage choice, and other factors…

  7. An observation tool for instructor and student behaviors to measure in-class learner engagement: a validation study

    Directory of Open Access Journals (Sweden)

    Mustafa K. Alimoglu

    2014-10-01

    Full Text Available Background: Efforts are made to enhance in-class learner engagement because it stimulates and enhances learning. However, it is not easy to quantify learner engagement. This study aimed to develop and validate an observation tool for instructor and student behaviors to determine and compare in-class learner engagement levels in four different class types delivered by the same instructor. Methods: Observer pairs observed instructor and student behaviors during lectures in large class (LLC, n=2 with third-year medical students, lectures in small class (LSC, n=6 and case-based teaching sessions (CBT, n=4 with fifth-year students, and problem-based learning (PBL sessions (~7 hours with second-year students. The observation tool was a revised form of STROBE, an instrument for recording behaviors of an instructor and four randomly selected students as snapshots for 5-min cycles. Instructor and student behaviors were scored 1–5 on this tool named ‘in-class engagement measure (IEM’. The IEM scores were parallel to the degree of behavior's contribution to active student engagement, so higher scores were associated with more in-class learner engagement. Additionally, the number of questions asked by the instructor and students were recorded. A total of 203 5-min observations were performed (LLC 20, LSC 85, CBT 50, and PBL 48. Results: Interobserver agreement on instructor and student behaviors was 93.7% (κ=0.87 and 80.6% (κ=0.71, respectively. Higher median IEM scores were found in student-centered and problem-oriented methods such as CBT and PBL. A moderate correlation was found between instructor and student behaviors (r=0.689. Conclusions: This study provides some evidence for validity of the IEM scores as a measure of student engagement in different class types.

  8. An observation tool for instructor and student behaviors to measure in-class learner engagement: a validation study.

    Science.gov (United States)

    Alimoglu, Mustafa K; Sarac, Didar B; Alparslan, Derya; Karakas, Ayse A; Altintas, Levent

    2014-01-01

    Efforts are made to enhance in-class learner engagement because it stimulates and enhances learning. However, it is not easy to quantify learner engagement. This study aimed to develop and validate an observation tool for instructor and student behaviors to determine and compare in-class learner engagement levels in four different class types delivered by the same instructor. Observer pairs observed instructor and student behaviors during lectures in large class (LLC, n=2) with third-year medical students, lectures in small class (LSC, n=6) and case-based teaching sessions (CBT, n=4) with fifth-year students, and problem-based learning (PBL) sessions (~7 hours) with second-year students. The observation tool was a revised form of STROBE, an instrument for recording behaviors of an instructor and four randomly selected students as snapshots for 5-min cycles. Instructor and student behaviors were scored 1-5 on this tool named 'in-class engagement measure (IEM)'. The IEM scores were parallel to the degree of behavior's contribution to active student engagement, so higher scores were associated with more in-class learner engagement. Additionally, the number of questions asked by the instructor and students were recorded. A total of 203 5-min observations were performed (LLC 20, LSC 85, CBT 50, and PBL 48). Interobserver agreement on instructor and student behaviors was 93.7% (κ=0.87) and 80.6% (κ=0.71), respectively. Higher median IEM scores were found in student-centered and problem-oriented methods such as CBT and PBL. A moderate correlation was found between instructor and student behaviors (r=0.689). This study provides some evidence for validity of the IEM scores as a measure of student engagement in different class types.

  9. Student Evaluation of Instruction: Comparison between In-Class and Online Methods

    Science.gov (United States)

    Capa-Aydin, Yesim

    2016-01-01

    This study compares student evaluations of instruction that were collected in-class with those gathered through an online survey. The two modes of administration were compared with respect to response rate, psychometric characteristics and mean ratings through different statistical analyses. Findings indicated that in-class evaluations produced a…

  10. Children's relative age in class and use of medication for ADHD: a Danish Nationwide Study

    DEFF Research Database (Denmark)

    Pottegård, Anton; Hallas, J.; Hernandez, Diaz

    2014-01-01

    estimated the prevalence proportion ratio (PPR) of receiving ADHD medication between the youngest children in class (born in October-December) and the oldest in class (born in January-March), specified by grade level, calendar year and gender. As a sensitivity analysis, we added children not on their age...

  11. Online Quizzes Promote Inconsistent Improvements on In-Class Test Performance in Introductory Anatomy and Physiology

    Science.gov (United States)

    Brown, Gregory A.; Bice, Matthew R.; Shaw, Brandon S.; Shaw, Ina

    2015-01-01

    Review quizzes can provide students with feedback and assist in the preparation for in-class tests, but students often do not voluntarily use self-testing resources. The purpose of the present study was to evaluate if taking a mandatory online review quiz alters performance on subsequent in-class tests. During two semesters of a single-semester…

  12. "Writing Wasn't Really Stressed, Accurate Historical Analysis Was Stressed": Student Perceptions of In-Class Writing in the Inverted, General Education, University History Survey Course

    Science.gov (United States)

    Murphree, Daniel S.

    2014-01-01

    Taking introductory history courses and writing analytical essays are not the favorite activities of most first-year university students. Undergraduates, seemingly, would rather enroll in classes that pertain only to their majors or job-preparation regimen. If forced to take General Education Program (GEP) courses, students typically favor those…

  13. Buried Waste Integrated Demonstration Strategy Plan

    International Nuclear Information System (INIS)

    Kostelnik, K.M.

    1993-02-01

    The Buried Waste Integrated Demonstration (BWID) supports the applied research, development, demonstration, and evaluation of a suite of advanced technologies that form a comprehensive remediation system for the effective and efficient remediation of buried waste. These efforts are identified and coordinated in support of the US Department of Energy (DOE), Environmental Restoration and Waste Management (ERWM) needs and objectives. The present focus of BWID is to support retrieval and ex situ treatment configuration options. Future activities will explore and support containment and stabilization efforts in addition to the retrieval/ex situ treatment options. Long and short term strategies of the BWID are provided. Processes for identifying technological needs, screening candidate technologies for BWID applicability, researching technical issues, field demonstrating technologies, evaluating demonstration results to determine each technology's threshold of capability, and commercializing successfully demonstrated technologies for implementation for environmental restoration also are presented in this report

  14. On Instantaneous Power Dissipation in Class B Amplifier

    Directory of Open Access Journals (Sweden)

    Hristo Zhivomirov

    2014-08-01

    Full Text Available The present paper describes the analysis of the instantaneous power dissipation by the two active components in a class B power amplifier. Attention is paid to restrictions of the instantaneous power dissipation relations in reference literature, and the consequences of their misuse. A new generalized equation that takes into account the power dissipated by the two active devices is proposed. The theoretical statement is substantiated by Matlab® numeric computation and visualization, Cadence OrCAD® simulations and measurements of a real-world audio power amplifier performed by NI USB-6211 measurement complex.

  15. Demonstration MTI/SAR capability

    NARCIS (Netherlands)

    Vries, F.P.P. de; Broek, A.C. van den; Otten, M.P.G.; Groot, J.S.; Steeghs, T.P.H.; Dekker, R.J.; Rossum, W.L. van

    2001-01-01

    The aim of this project is to demonstrate to the Dutch armed forces the capability of MTI (Moving Target Indicator) and SAR (Synthetic Aperture Radar). This is done with the Dutch PHARUS sensor. The sensor is used to demonstrate how a phased array antenna can be used as an MTI/SAR sensor

  16. A Comprehensive General Chemistry Demonstration

    Science.gov (United States)

    Sweeder, Ryan D.; Jeffery, Kathleen A.

    2013-01-01

    This article describes the use of a comprehensive demonstration suitable for a high school or first-year undergraduate introductory chemistry class. The demonstration involves placing a burning candle in a container adjacent to a beaker containing a basic solution with indicator. After adding a lid, the candle will extinguish and the produced…

  17. Optics Demonstrations Using Cylindrical Lenses

    Science.gov (United States)

    Ivanov, Dragia; Nikolov, Stefan

    2015-01-01

    In this paper we consider the main properties of cylindrical lenses and propose several demonstrational experiments that can be performed with them. Specifically we use simple glasses full of water to demonstrate some basic geometrical optics principles and phenomena. We also present some less standard experiments that can be performed with such…

  18. Rocket Ignition Demonstrations Using Silane

    Science.gov (United States)

    Pal, Sibtosh; Santoro, Robert; Watkins, William B.; Kincaid, Kevin

    1998-01-01

    Rocket ignition demonstration tests using silane were performed at the Penn State Combustion Research Laboratory. A heat sink combustor with one injection element was used with gaseous propellants. Mixtures of silane and hydrogen were used as fuel, and oxygen was used as oxidizer. Reliable ignition was demonstrated using fuel lead and and a swirl injection element.

  19. Teaching and Demonstrating Classical Conditioning.

    Science.gov (United States)

    Sparrow, John; Fernald, Peter

    1989-01-01

    Discusses classroom demonstrations of classical conditioning and notes tendencies to misrepresent Pavlov's procedures. Describes the design and construction of the conditioner that is used for demonstrating classical conditioning. Relates how students experience conditioning, generalization, extinction, discrimination, and spontaneous recovery.…

  20. Classroom Demonstrations of Wood Properties.

    Science.gov (United States)

    Foulger, A. N.

    Presented in this manual are 20 activities selected to show some of the properties of wood and how these properties relate to the cellular structure of wood. Each activity includes stated objectives, indicates materials needed, and explains procedures. Illustrations related to the activities, glossary of terms, and photographs of wood structure…

  1. Modeling Distortion Effects in Class-D Amplifier Filter Inductors

    DEFF Research Database (Denmark)

    Knott, Arnold; Stegenborg-Andersen, Tore; Thomsen, Ole Cornelius

    2010-01-01

    Distortion is generally accepted as a quantifier to judge the quality of audio power amplifiers. In switchmode power amplifiers various mechanisms influence this performance measure. After giving an overview of those, this paper focuses on the particular effect of the nonlinearity of the output...... filter components on the audio performance. While the physical reasons for both, the capacitor and the inductor induced distortion are given, the practical in depth demonstration is done for the inductor only. This includes measuring the inductors performance, modeling through fitting and resulting...... into simulation models. The fitted models achieve distortion values between 0.03 % and 0.2 % as a basis to enable the design of a 200 W amplifier....

  2. Efficiency Optimization in Class-D Audio Amplifiers

    DEFF Research Database (Denmark)

    Yamauchi, Akira; Knott, Arnold; Jørgensen, Ivan Harald Holger

    2015-01-01

    This paper presents a new power efficiency optimization routine for designing Class-D audio amplifiers. The proposed optimization procedure finds design parameters for the power stage and the output filter, and the optimum switching frequency such that the weighted power losses are minimized under...... the given constraints. The optimization routine is applied to minimize the power losses in a 130 W class-D audio amplifier based on consumer behavior investigations, where the amplifier operates at idle and low power levels most of the time. Experimental results demonstrate that the optimization method can...... lead to around 30 % of efficiency improvement at 1.3 W output power without significant effects on both audio performance and the efficiency at high power levels....

  3. Optical demonstration on Honeywell FLASH program

    Science.gov (United States)

    O'Rourke, Ken; Peterson, Eric

    1996-10-01

    Flight control system architects are increasingly integrating aircraft control and management functionality. This functional integration increases performance and cost of ownership requirements on the communication pathways. Optical signal transmission is an attractive approach to satisfying these system requirements. Investigations and demonstrations of optic signaling are needed to validate applicability of fiber based communication systems to the new system architecture performance and cost requirements. Honeywell's activities associated with the Fly-By-Light Advanced System Hardware (FLASH) program have produced a system including key optical interface elements and accomplished preliminary demonstrations needed to validate flexible, optical based aircraft control and avionic systems. The Honeywell Primary Flight Control System included Active Hand Controllers, Primary Flight Control Computers and smart actuation subsystem elements interfaced through various optical implementations and communication protocols. These successful implementations and demonstrations provide an excellent baseline for the processes, tools and materials required to make Fly-by-Light avionic systems marketable.

  4. Cubesat Proximity Operations Demonstration (CPOD)

    Science.gov (United States)

    Villa, Marco; Martinez, Andres; Petro, Andrew

    2015-01-01

    The CubeSat Proximity Operations Demonstration (CPOD) project will demonstrate rendezvous, proximity operations and docking (RPOD) using two 3-unit (3U) CubeSats. Each CubeSat is a satellite with the dimensions 4 inches x 4 inches x 13 inches (10 centimeters x 10 centimeters x 33 centimeters) and weighing approximately 11 pounds (5 kilograms). This flight demonstration will validate and characterize many new miniature low-power proximity operations technologies applicable to future missions. This mission will advance the state of the art in nanosatellite attitude determination,navigation and control systems, in addition to demonstrating relative navigation capabilities.The two CPOD satellites are scheduled to be launched together to low-Earth orbit no earlier than Dec. 1, 2015.

  5. Spyder: Critical Technology Demonstration Tests

    Data.gov (United States)

    National Aeronautics and Space Administration — Two technology demonstrations:Task 1 – Sub-orbital hot fire staging with guidance and control utilizing NASA-Ames AVA. Task 2 – Spyder stage 1 static test, nose...

  6. Rubens Flame-Tube Demonstration.

    Science.gov (United States)

    Ficken, George W.; Stephenson, Francis C.

    1979-01-01

    Investigates and explains the phenomenon associated with Rubens flame-tube demonstration, specifically the persistance of flames at regular intervals along the tube for few minutes after the gas is turned off. (GA)

  7. Status of the Majorana Demonstrator

    Energy Technology Data Exchange (ETDEWEB)

    Cuesta, C.; Abgrall, N.; Arnquist, Isaac J.; Avignone, Frank T.; Barabash, Alexander S.; Bertrand, F.; Brudanin, V.; Busch, Matthew; Buuck, M.; Byram, D.; Caldwell, A. S.; Chan, Yuen-Dat; Christofferson, Cabot-Ann; Detwiler, Jason A.; Efremenko, Yuri; Ejiri, H.; Elliott, S. R.; Galindo-Uribarri, A.; Giovanetti, G. K.; Goett, J.; Green, M. P.; Gruszko, J.; Guinn, I.; Guiseppe, V. E.; Henning, R.; Hoppe, Eric W.; Howard, Stanley; Howe, M. A.; Jasinski, B. R.; Keeter, K.; Kidd, M. F.; Konovalov, S.; Kouzes, Richard T.; Laferriere, Brian D.; Leon, Jonathan D.; MacMullin, J.; Martin, R. D.; Meijer, S. J.; Mertens, S.; Orrell, John L.; O' Shaughnessy, C.; Overman, Nicole R.; Poon, Alan; Radford, D. C.; Rager, J.; Rielage, Keith; Robertson, R. G. H.; Romero-Romero, E.; Schmitt, C.; Shanks, B.; Shirchenko, M.; Snyder, N.; Suriano, Anne-Marie; Tedeschi, D.; Timkin, V.; Trimble, J. E.; Varner, R. L.; Vasilyev, Sergey; Vetter, Kai; Vorren, Kris R.; White, Brandon R.; Wilkerson, J. F.; Wiseman, C.; Xu, W.; Yakushev, E.; Yu, Chang-Hong; Yumatov, Vladimir

    2015-06-09

    The Majorana Collaboration is constructing the Majorana Demonstrator, an ultra-low background, 40-kg modular high purity Ge detector array to search for neutrinoless double-beta decay in 76Ge. In view of the next generation of tonne-scale Ge-based neutrinoless double-beta decay searches that will probe the neutrino mass scale in the inverted hierarchy region, a major goal of the Demonstrator is to demonstrate a path forward to achieving a background rate at or below 1 count/tonne/year in the 4 keV region of interest around the Q-value at 2039 keV. The current status of the Demonstrator is discussed, as are plans for its completion.

  8. CT Demonstration of Caput Medusae

    Science.gov (United States)

    Weber, Edward C.; Vilensky, Joel A.

    2009-01-01

    Maximum intensity and volume rendered CT displays of caput medusae are provided to demonstrate both the anatomy and physiology of this portosystemic shunt associated with portal hypertension. (Contains 2 figures.)

  9. Fuel Gas Demonstration Plant Program. Volume I. Demonstration plant

    Energy Technology Data Exchange (ETDEWEB)

    1979-01-01

    The objective of this project is for Babcock Contractors Inc. (BCI) to provide process designs, and gasifier retort design for a fuel gas demonstration plant for Erie Mining Company at Hoyt Lake, Minnesota. The fuel gas produced will be used to supplement natural gas and fuel oil for iron ore pellet induration. The fuel gas demonstration plant will consist of five stirred, two-stage fixed-bed gasifier retorts capable of handling caking and non-caking coals, and provisions for the installation of a sixth retort. The process and unit design has been based on operation with caking coals; however, the retorts have been designed for easy conversion to handle non-caking coals. The demonstration unit has been designed to provide for expansion to a commercial plant (described in Commercial Plant Package) in an economical manner.

  10. Photonic based marine radar demonstrator

    OpenAIRE

    Laghezza, Francesco; Scotti, Filippo; Ghelfi, Paolo; Bogoni, Antonella; Banchi, Luca; Malaspina, Vincenzo; Serafino, Giovanni

    2015-01-01

    This paper presents the results obtained during the field trial experiments of the first photonic-based radar system demonstrator, in a real maritime environment. The developed demonstrator exploits photonic technologies for both the generation and the detection of radar RF signals, allowing increased performance even in term of system flexibility. The photonic radar performance have been compared with a state of the art commercial system for maritime applications provide...

  11. In-Class Cycling to Augment College Student Academic Performance and Reduce Physical Inactivity: Results from an RCT

    Directory of Open Access Journals (Sweden)

    Lanae Joubert

    2017-11-01

    Full Text Available Most college students sit 14 hours per week on average, excluding sedentary study time. Researchers observing workplace and elementary school settings with active workstations to combat sedentary behavior have shown enhanced cognition without distraction. Until now, incorporating active workstations in college classroom settings remained relatively unexplored. This study’s purpose was to assess academic performance using in-class stationary cycle desks during a semester-long lecture course. Twenty-one college students (19–24 years enrolled in a lecture course volunteered and were split into traditional sit (SIT and stationary cycle (CYC groups randomly, matched on a calculated factor equal to a physical activity (PA score (0–680 multiplied by grade point average (GPA; 4.0 scale. CYC pedaled a prescribed rate of perceived exertion (RPE of less than 2 out of 10 during a 50-min lecture, 3 × week for 12 weeks. CYC averaged 42 min, 7.9 miles, and 1.7 RPE during class throughout the semester. No significant differences (p > 0.05 were observed between CYC and SIT on in-class test scores or overall course grades. Although statistically insignificant, CYC had higher mean test scores and overall course grades vs. SIT (i.e., B+ vs. B, respectively. Low intensity cycling during a college lecture course maintained student academic performance and possibly reduced weekly sedentary behavior time.

  12. In-Class Cycling to Augment College Student Academic Performance and Reduce Physical Inactivity: Results from an RCT.

    Science.gov (United States)

    Joubert, Lanae; Kilgas, Matthew; Riley, Alexandrea; Gautam, Yuba; Donath, Lars; Drum, Scott

    2017-11-04

    Most college students sit 14 hours per week on average, excluding sedentary study time. Researchers observing workplace and elementary school settings with active workstations to combat sedentary behavior have shown enhanced cognition without distraction. Until now, incorporating active workstations in college classroom settings remained relatively unexplored. This study's purpose was to assess academic performance using in-class stationary cycle desks during a semester-long lecture course. Twenty-one college students (19-24 years) enrolled in a lecture course volunteered and were split into traditional sit (SIT) and stationary cycle (CYC) groups randomly, matched on a calculated factor equal to a physical activity (PA) score (0-680) multiplied by grade point average (GPA; 4.0 scale). CYC pedaled a prescribed rate of perceived exertion (RPE) of less than 2 out of 10 during a 50-min lecture, 3 × week for 12 weeks. CYC averaged 42 min, 7.9 miles, and 1.7 RPE during class throughout the semester. No significant differences ( p > 0.05) were observed between CYC and SIT on in-class test scores or overall course grades. Although statistically insignificant, CYC had higher mean test scores and overall course grades vs. SIT (i.e., B⁺ vs. B, respectively). Low intensity cycling during a college lecture course maintained student academic performance and possibly reduced weekly sedentary behavior time.

  13. Microleakage of hydrophilic adhesive systems in Class V composite restorations.

    Science.gov (United States)

    Amaral, C M; Hara, A T; Pimenta, L A; Rodrigues, A L

    2001-02-01

    To investigate the microleakage of four hydrophilic adhesive systems: one "multiple-bottles" (Scotchbond Multi-Purpose Plus); two "one-bottle" (Single Bond, Stae); and one self-etching (Etch & Prime 3.0). 120 bovine incisor teeth were divided into four groups (n = 30) and Class V cavities were prepared at the cemento-enamel junction. The cavities were restored with the adhesive systems and with Z100 composite. The teeth were thermocycled 1,000 times between 5+/-2 degrees C and 55+/-2 degrees C with a dwell time of 1 min, and then placed in a 2% methylene blue dye (pH 7.0) for 4 hrs, washed and sectioned vertically through the center of the restorations. The qualitative evaluation was made by three examiners who distributed pre-established scores (0-4) for each tooth using a stereomicroscope at x30 magnification. In enamel margins little microleakage was observed and the Kruskal-Wallis analysis did not show differences. In dentin margins the Kruskal-Wallis and multiple comparison analyses were applied: microleakage was significantly greater with Stae (median 3) and Scotchbond MP Plus (median 4). Single Bond (median 1) and Etch & Prime 3.0 (median 2) showed the best results in dentin margins, and the statistical analysis did not demonstrate differences in microleakage among these groups.

  14. Telavancin demonstrates activity against methicillin-resistant Staphylococcus aureus isolates with reduced susceptibility to vancomycin, daptomycin, and linezolid in broth microdilution MIC and one-compartment pharmacokinetic/pharmacodynamic models.

    Science.gov (United States)

    Smith, Jordan R; Barber, Katie E; Hallesy, Jessica; Raut, Animesh; Rybak, Michael J

    2015-09-01

    Methicillin-resistant Staphylococcus aureus (MRSA) isolates have arisen with reduced susceptibility to several anti-MRSA agents. Telavancin (TLV), a novel anti-MRSA agent, retains low MICs against these organisms. Our objective was to determine the MICs for TLV, daptomycin (DAP), vancomycin (VAN), and linezolid (LZD) against daptomycin-nonsusceptible (DNS) S. aureus, vancomycin-intermediate S. aureus (VISA), heteroresistant VISA (hVISA), and linezolid-resistant (LZD(r)) S. aureus. We also evaluated these agents against each phenotype in pharmacokinetic/pharmacodynamic (PK/PD) models. Seventy DNS, 100 VISA, 180 hVISA, and 25 LZD(r) MRSA isolates were randomly selected from our library and tested to determine their MICs against TLV, DAP, VAN, and LZD via broth microdilution and a Trek panel. Four isolates were randomly selected for 168-h in vitro models to evaluate treatment with TLV at 10 mg/kg of body weight/day, DAP at 10 mg/kg/day, VAN at 1 g every 12 h (q12h), and LZD at 600 mg q12h. The MIC50/90 for TLV, DAP, VAN, and LZD against 70 DNS S. aureus isolates were 0.06/0.125 μg/ml, 2/4 μg/ml, 1/2 μg/ml, and 2/2 μg/ml, respectively. Against 100 VISA isolates, the MIC50/90 were 0.06/0.125 μg/ml, 1/1 μg/ml, 4/8 μg/ml, and 1/2 μg/ml, respectively. Against 170 hVISA isolates, the MIC50/90 were 0.06/0.125 μg/ml, 0.5/1 μg/ml, 1/2 μg/ml, and 1/2 μg/ml, respectively. Against 25 LZD(r) isolates, the MIC50/90 were 0.03/0.06 μg/ml, 1/1 μg/ml, 2/2 μg/ml, and 8/8 μg/ml, respectively. The TLV MIC was >0.125 μg/ml for 10/365 (2.7%) isolates. In PK/PD models, TLV was universally bactericidal at 168 h and statistically superior to all antibiotics against DNS S. aureus strain R2334. These data further establish the potency of TLV against resistant MRSA. The model data demonstrate in vitro bactericidal activity of TLV against hVISA, VISA, DNS S. aureus, and LZD(r) S. aureus strains. Further clinical research is warranted. Copyright © 2015, American Society for

  15. Status of the MAJORANA DEMONSTRATOR

    Energy Technology Data Exchange (ETDEWEB)

    Cuesta, C [University of Washington, Seattle; Abgrall, N. [Lawrence Berkeley National Laboratory (LBNL); Arnquist, I. J. [Pacific Northwest National Laboratory (PNNL); Avignone, III, F. T. [University of South Carolina/Oak Ridge National Laboratory (ORNL); Baldenegro-Barrera, C. X. [Oak Ridge National Laboratory (ORNL); Barabash, A.S. [Institute of Theoretical & Experimental Physics (ITEP), Moscow, Russia; Bertrand, F. E. [Oak Ridge National Laboratory (ORNL); Bradley, A. W. [Lawrence Berkeley National Laboratory (LBNL); Brudanin, V. [Joint Institute for Nuclear Research, Dubna, Russia; Busch, M. [Duke University/TUNL; Buuck, M. [University of Washington, Seattle; Byram, D. [University of South Dakota; Caldwell, Adam S [South Dakota School of Mines and Technology; Chan, Y-D [Lawrence Berkeley National Laboratory (LBNL); Christofferson, C. D. [South Dakota School of Mines and Technology; Chu, P.-H. [Los Alamos National Laboratory (LANL); Detwiler, J. A. [University of Washington, Seattle; Efremenko, Yu. [University of Tennessee, Knoxville (UTK); Ejiri, H. [Osaka University, Japan; Elliott, S. R. [Los Alamos National Laboratory (LANL); Galindo-Uribarri, A [Oak Ridge National Laboratory (ORNL); Gilliss, T. [Univ. North Carolina-Chapel Hill/Triangle Univ. Nucl. Lab., Durham, NC; Giovanetti, G. K. [University of North Carolina / Triangle Universities Nuclear Lababoratory, Durham; Goett, J [Los Alamos National Laboratory (LANL); Green, M. P. [Oak Ridge National Laboratory (ORNL); Gruszko, J [University of Washington, Seattle; Guinn, I S [University of Washington, Seattle; Guiseppe, V E [University of South Carolina, Columbia; Henning, R. [University of North Carolina / Triangle Universities Nuclear Lababoratory, Durham; Hoppe, E.W. [Pacific Northwest National Laboratory (PNNL); Howard, S. [South Dakota School of Mines and Technology; Howe, M. A. [University of North Carolina / Triangle Universities Nuclear Lababoratory, Durham; Jasinski, B R [University of South Dakota; Keeter, K.J. [Black Hills State University, Spearfish, South Dakota; Kidd, M. F. [Tennessee Technological University (TTU); Konovalov, S.I. [Institute of Theoretical & Experimental Physics (ITEP), Moscow, Russia; Kouzes, R. T. [Pacific Northwest National Laboratory (PNNL); LaFerriere, B. D. [Pacific Northwest National Laboratory (PNNL); Leon, J. [University of Washington, Seattle; MacMullin, J. [University of North Carolina / Triangle Universities Nuclear Lababoratory, Durham; Martin, R. D. [University of South Dakota; Massarczyk, R. [Los Alamos National Laboratory (LANL); Meijer, S. J. [University of North Carolina / Triangle Universities Nuclear Lababoratory, Durham; Mertens, S. [Lawrence Berkeley National Laboratory (LBNL); Orrell, J. L. [Pacific Northwest National Laboratory (PNNL); O' Shaughnessy, C. [Univ. North Carolina-Chapel Hill/Triangle Univ. Nucl. Lab., Durham, NC; Poon, A.W.P. [Lawrence Berkeley National Laboratory (LBNL); Radford, D. C. [Oak Ridge National Laboratory (ORNL); Rager, J. [Univ. North Carolina-Chapel Hill/Triangle Univ. Nucl. Lab., Durham, NC; Rielage, K. [Los Alamos National Laboratory (LANL); Robertson, R.G.H. [University of Washington, Seattle; Romero-Romero, E. [University of Tennessee, Knoxville, (UTK)/Oak Ridge National Lab (ORNL); Shanks, B. [Univ. North Carolina-Chapel Hill/Triangle Univ. Nucl. Lab., Durham, NC; Shirchenko, M. [Joint Institute for Nuclear Research, Dubna, Russia; Snyder, N [University of South Dakota; Suriano, A. M. [South Dakota School of Mines and Technology; Tedeschi, D [University of South Carolina, Columbia; Trimble, J. E. [Univ. North Carolina-Chapel Hill/Triangle Univ. Nucl. Lab., Durham, NC; Varner, R. L. [Oak Ridge National Laboratory (ORNL); Vasilyev, S. [Joint Institute for Nuclear Research, Dubna, Russia; et al.

    2015-01-01

    The MAJORANA Collaboration is constructing the MAJORANA DEMONSTRATOR, an ultra-low background, modular, HPGe detector array with a mass of 44-kg (29 kg Ge-76 and 15 kg Ge-nat) to search for neutrinoless double beta decay in Ge-76. The next generation of tonne-scale Ge-based neutrinoless double beta decay searches will probe the neutrino mass scale in the inverted-hierarchy region. The MAJORANA DEMONSTRATOR is envisioned to demonstrate a path forward to achieve a background rate at or below 1 count/tonne/year in the 4 keV region of interest around the Q-value of 2039 keV. The MAJO-RANA DEMONSTRATOR follows a modular implementation to be easily scalable to the next generation experiment. First, the prototype module was assembled; it has been continuously taking data from July 2014 to June 2015. Second, Module 1 with more than half of the total enriched detectors and some natural detectors has been assembled and it is being commissioned. Finally, the assembly of Module 2, which will complete MAJORANA DEMONSTRATOR, is already in progress.

  16. Auditory demonstrations simulating Mayan architecture

    Science.gov (United States)

    Lubman, David

    2005-09-01

    Fascination with the ancient temples and ball court at Chichen Itza provide rich opportunities for science education. Children of all ages are delighted to learn that the sound of handclaps scattered from long temple staircases are transformed into bird chirps. Their engagement in such seemingly magical phenomena provides magic moments for teaching acoustical principals, including the picket-fence effect (PFE). PFE transforms impulsive sounds scattered from spatially periodic structures into tonal sounds. PFE is demonstrated with a computer possessing a sound card and a simple sound editing program. The inverse relationship between tonal frequency and the time interval between periodic impulses is easily demonstrated. The number of impulses needed to produce an audible tone is easily demonstrated and compared with the number of steps on the staircase. Transformation of audible tones into downward-gliding chirps is simulated by monotonically increasing the time between impulses. The Great Ball Court also provides opportunities for acoustical demonstration. Observers clapping their hands while standing between the long, tall, and parallel walls of the playing field marvel at the profound flutter echo heard for about 1.5 s. The flutter echo sonogram demonstrates the speed of sound and frequency-selective atmospheric attenuation.

  17. Demonstration of blind quantum computing.

    Science.gov (United States)

    Barz, Stefanie; Kashefi, Elham; Broadbent, Anne; Fitzsimons, Joseph F; Zeilinger, Anton; Walther, Philip

    2012-01-20

    Quantum computers, besides offering substantial computational speedups, are also expected to preserve the privacy of a computation. We present an experimental demonstration of blind quantum computing in which the input, computation, and output all remain unknown to the computer. We exploit the conceptual framework of measurement-based quantum computation that enables a client to delegate a computation to a quantum server. Various blind delegated computations, including one- and two-qubit gates and the Deutsch and Grover quantum algorithms, are demonstrated. The client only needs to be able to prepare and transmit individual photonic qubits. Our demonstration is crucial for unconditionally secure quantum cloud computing and might become a key ingredient for real-life applications, especially when considering the challenges of making powerful quantum computers widely available.

  18. Savannah River Plant incinerator demonstration

    International Nuclear Information System (INIS)

    Lewandowski, K.E.

    1983-01-01

    A full-scale incineration process was demonstrated at the Savannah River Laboratory (SRL) using nonradioactive waste. From October 1981 through September 1982, 15,700 kilograms of solid waste and 5.7 m 3 of solvent were incinerated. Emissions of off-gas components (NO/sub x/, SO 2 , CO, and particulates) were well below South Carolina state standards. Volume reductions of 20:1 for solid waste and 7:1 for Purex solvent/lime slurry were achieved. The process has been relocated and upgraded by the Savannah River Plant to accept low-level beta-gamma combustibles. During a two-year demonstration, the facility will incinerate slightly radioactive ( 3 ) solvent and suspect level (< 1 mR/h at 0.0254 meter) solid wastes. This demonstration will begin in early 1984

  19. DEMONSTRATION BULK VITRIFICATION SYSTEM (DBVS) EXTERNAL REVIEW

    International Nuclear Information System (INIS)

    HONEYMAN, J.O.

    2007-01-01

    The Hanford mission to retrieve and immobilize 53 million gallons of radioactive waste from 177 underground storage tanks will be accomplished using a combination of processing by the waste treatment plant currently under construction, and a supplemental treatment that would process low-activity waste. Under consideration for this treatment is bulk vitrification, a versatile joule-heated melter technology which could be deployed in the tank farms. The Department proposes to demonstrate this technology under a Research, Development and Demonstration (RD and D) permit issued by the Washington State Department of Ecology using both non-radioactive simulant and blends of actual tank waste. From the demonstration program, data would be obtained on cost and technical performance to enable a decision on the potential use of bulk vitrification as the supplemental treatment technology for Hanford. An independent review by sixteen subject matter experts was conducted to assure that the technical basis of the demonstration facility design would be adequate to meet the objectives of the Demonstration Bulk Vitrification System (DBVS) program. This review explored all aspects of the program, including flowsheet chemistry, project risk, vitrification, equipment design and nuclear safety, and was carried out at a time when issues can be identified and corrected. This paper describes the mission need, review approach, technical recommendations and follow-on activities for the DBVS program

  20. College Can Fire Teacher for Swearing at Student in Class, U.S. Court Rules.

    Science.gov (United States)

    Mangan, Katherine S.

    1987-01-01

    An appellate court found that a college faculty member's use of offensive language in class was unprofessional, interfered with instruction, and not protected by principles of free speech and academic freedom. (MSE)

  1. Representation of Power in Class Discourse: A Study of Communication Ethnography

    Directory of Open Access Journals (Sweden)

    Jumadi Jumadi

    2016-02-01

    Full Text Available This study is aimed at describing the use of power in class discourse covering representation of power in speech act, representation of power in communication patterns and function of power in class discourse. Communication ethnography and pragmatic methods were used. The research results show that the use of directive, assertive, and expressive acts in class discourse represent the power with certain domination; that the control of speech topics, interruptions, and overlapping tend to represent the power with certain domination that also effect the legitimacy of the user of this strategy. In relation to its function, power in class discourse is applied as a preventive, supportive, and corrective acts in order to achieve instructional objective.

  2. Field demonstration of an active reservoir pressure management through fluid injection and displaced fluid extractions at the Rock Springs Uplift, a priority geologic CO2 storage site for Wyoming

    Energy Technology Data Exchange (ETDEWEB)

    Jiao, Zunsheng [Univ. of Wyoming, Laramie, WY (United States)

    2017-04-05

    This report provides the results from the project entitled Field Demonstration of Reservoir Pressure Management through Fluid Injection and Displaced Fluid Extraction at the Rock Springs Uplift, a Priority Geologic CO2 Storage Site for Wyoming (DE-FE0026159 for both original performance period (September 1, 2015 to August 31, 2016) and no-cost extension (September 1, 2016 to January 6, 2017)).

  3. 76 FR 60007 - TRICARE Demonstration Project for the Philippines

    Science.gov (United States)

    2011-09-28

    ... DEPARTMENT OF DEFENSE Office of the Secretary TRICARE Demonstration Project for the Philippines... Activity. ACTION: Notice of a TRICARE demonstration project for the Philippines. SUMMARY: This notice is to... Demonstration Project for the Philippines.'' The purpose of this demonstration is to validate an alternative...

  4. INVESTIGATING TURNTAKING STRATEGIES IN CLASS DISCUSSIONS AMONG ESL ADULT LEARNERS

    Directory of Open Access Journals (Sweden)

    Noor Hanim Rahmat

    2015-06-01

    Full Text Available This quantitative study looked into class discussion strategies used by adult ESL learners. Findings revealed several benefits of class discussions for adult learners. Among some of them are, adult learners practice turn-taking skills in a non-combative environment. The sharing of ideas allowed freedom of thoughts among the learners. On the other hand, adult learners also learn to agree or disagree politely using turn-taking strategies. Finally, the benefits of class discussions may go beyond classroom needs such as improving critical thinking skills among learners. This ability is seen when learners participate in group work and discussions. Learners gain accessibility to participate comfortably in discussions when they are pun in a non-combative environment. This will thus give them the freedom to discuss any topics openly. This freedom will further enhance their general participation in the discussion. However, in a normal conversational process, speakers need to learn to speak up and also to give others space to voice their opinions. Learners need the knowledge of turn-taking skills in order to participate actively in the discussion. This turn-taking skills can be taught in ESL classrooms through class discussions.

  5. Environmental management technology demonstration and commercialization

    International Nuclear Information System (INIS)

    Daly, D.J.; Erickson, T.A.; Groenewold, G.H.

    1995-01-01

    The Energy ampersand Environmental Research Center (EERC), a contract-supported organization focused on technology research, development, demonstration, and commercialization (RDD ampersand C), is entering its second year of a Cooperative Agreement with the U.S. Department of Energy (DOE) Morgantown Energy Technology Center (METC) to facilitate the development, demonstration, and commercialization of innovative environmental management (EM) technologies in support of the activities of DOE's Office of Environmental Science and Technology (EM-50) under DOE's EM Program. This paper reviews the concept and approach of the program under the METC-EERC EM Cooperative Agreement and profiles the role the program is playing in the commercialization of five EM technologies

  6. Demonstration of safety for geologic disposal

    International Nuclear Information System (INIS)

    Taylor, E.C.; Ramspott, L.D.; Sprecher, W.M.

    1994-01-01

    The US Department of Energy (DOE) is developing a nuclear waste management system that will accept high-level radioactive waste, transport it, store it, and ultimately emplace it in a deep geologic repository. The key activity now is determining whether Yucca Mountain, Nevada is suitable as a site for the repository. If so, the crucial technological advance will be the demonstration that disposal of nuclear waste will be safe for thousands of years after closure. This paper assesses the impact of regulatory developments, legal developments, and scientific developments on such a demonstration

  7. Engaged Journalism: Using Experiential Learning Theory (ELT) for In-Class Journaling Activities

    Science.gov (United States)

    Jenkins, J. Jacob; Clarke, Tracylee

    2017-01-01

    Educators have long recognized the value and import of class journaling. Traditional approaches to journaling, however, only engage students in one mode of communicative expression while allowing them to procrastinate in writing their entries. Typical journals are also read exclusively by the instructor, which overlooks the opportunity for…

  8. Soft tissue changes inconclusive in Class II division 1 patients treated with Activator and Bionator appliances

    NARCIS (Netherlands)

    Ren, Yijin

    2007-01-01

    DATA SOURCES: Medline, Medline In-Process and other non-indexed citations, Lilacs, PubMed, Embase, Web of Science and all evidence-based medicine reviews were searched. The reference lists of the retrieved articles were also searched by hand for possible missing articles. Authors were contacted to

  9. Some Field Demonstrations in India

    Indian Academy of Sciences (India)

    First page Back Continue Last page Graphics. Some Field Demonstrations in India. 2x150kVAR STATCOM at M/s Hindusthan Latex, Trivandrum. 250kVAR, 800V dc, 2-level STATCOM (Installed at Peekey Steels, Calicut). 250kVAR,800V dc, UPQC at CDAC, Trivandrum. REFERENCE: Website www. cdac.gov.in.

  10. Phenolphthalein-Pink Tornado Demonstration

    Science.gov (United States)

    Prall, Bruce R.

    2008-01-01

    The titration of HCl with NaOH has traditionally been used to introduce beginning chemistry students to the concepts of acid-base chemistry and stoichiometry. The demonstration described in this article utilizes this reaction as a means of providing students an opportunity to observe the dynamic motion associated with a swirling vortex and its…

  11. Demonstrating Fermat's Principle in Optics

    Science.gov (United States)

    Paleiov, Orr; Pupko, Ofir; Lipson, S. G.

    2011-01-01

    We demonstrate Fermat's principle in optics by a simple experiment using reflection from an arbitrarily shaped one-dimensional reflector. We investigated a range of possible light paths from a lamp to a fixed slit by reflection in a curved reflector and showed by direct measurement that the paths along which light is concentrated have either…

  12. Flexible-Rotor Balancing Demonstration

    Science.gov (United States)

    Giordano, J.; Zorzi, E.

    1986-01-01

    Report describes method for balancing high-speed rotors at relatively low speeds and discusses demonstration of method on laboratory test rig. Method ensures rotor brought up to speeds well over 20,000 r/min smoothly, without excessive vibration amplitude at critical speeds or at operating speed.

  13. E/Z MAS demonstration

    International Nuclear Information System (INIS)

    Boor, M.G.; Hurford, J.M.; Landry, R.P.; Martinez, B.J.; Solem, A.M.; Whiteson, R.; Zardecki, A.

    1998-01-01

    Los Alamos National Laboratory has developed E/Z MAS, a new generation nuclear material accountability application based on the latest technology and designed for facilities required to track nuclear materials with a simple-to-use interface. E/Z MAS is based on years of experience spent developing nuclear material accounting systems. E/Z MAS uses a modern relational database with a web server and enables users on a classified local area network to interact with the database with web browsers. The E/Z MAS Demonstration poster session demonstrates the E/Z MAS functions required by an operational nuclear facility to track material as it enters and leaves a facility and to account for the material as it moves through a process. The generation of internal facility reports and external reports for the Russian Federal system will be demonstrated. Bar-code readers will be used to demonstrate the ability of EZ MAS to automate certain functions, such as physical inventories at facilities

  14. In Situ Wetland Restoration Demonstration

    Science.gov (United States)

    2016-06-01

    applied to the soil (see Figure 5-3). iv. The benthic community was sampled for abundance, taxa richness, Biotic Index, functional feeding groups ...1 Figure 1-2 Freshwater Tidal Wetland...process (Chadwick, 2008). Figure 1-2 Freshwater Tidal Wetland ESTCP Project ER-200825 In Situ Wetland Restoration Demonstration ER-200825 Final

  15. SunJammer Technology Demonstration

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Sunjammer Project is a NASA funded contract to L?Garde Inc. to fly a solar sail demonstration for a period of approximately one year. L?Garde is also partnered...

  16. Satellite Demonstration: The Videodisc Technology.

    Science.gov (United States)

    Propp, George; And Others

    1979-01-01

    Originally part of a symposium on educational media for the deaf, the paper describes a satellite demonstration of video disc materials. It is explained that a panel of deaf individuals in Washington, D.C. and another in Nebraska came into direct two-way communication for the first time, and video disc materials were broadcast via the satellite.…

  17. DOE's annealing prototype demonstration projects

    International Nuclear Information System (INIS)

    Warren, J.; Nakos, J.; Rochau, G.

    1997-01-01

    One of the challenges U.S. utilities face in addressing technical issues associated with the aging of nuclear power plants is the long-term effect of plant operation on reactor pressure vessels (RPVs). As a nuclear plant operates, its RPV is exposed to neutrons. For certain plants, this neutron exposure can cause embrittlement of some of the RPV welds which can shorten the useful life of the RPV. This RPV embrittlement issue has the potential to affect the continued operation of a number of operating U.S. pressurized water reactor (PWR) plants. However, RPV material properties affected by long-term irradiation are recoverable through a thermal annealing treatment of the RPV. Although a dozen Russian-designed RPVs and several U.S. military vessels have been successfully annealed, U.S. utilities have stated that a successful annealing demonstration of a U.S. RPV is a prerequisite for annealing a licensed U.S. nuclear power plant. In May 1995, the Department of Energy's Sandia National Laboratories awarded two cost-shared contracts to evaluate the feasibility of annealing U.S. licensed plants by conducting an anneal of an installed RPV using two different heating technologies. The contracts were awarded to the American Society of Mechanical Engineers (ASME) Center for Research and Technology Development (CRTD) and MPR Associates (MPR). The ASME team completed its annealing prototype demonstration in July 1996, using an indirect gas furnace at the uncompleted Public Service of Indiana's Marble Hill nuclear power plant. The MPR team's annealing prototype demonstration was scheduled to be completed in early 1997, using a direct heat electrical furnace at the uncompleted Consumers Power Company's nuclear power plant at Midland, Michigan. This paper describes the Department's annealing prototype demonstration goals and objectives; the tasks, deliverables, and results to date for each annealing prototype demonstration; and the remaining annealing technology challenges

  18. Science Learning: A path analysis of its links with reading comprehension, question-asking in class and science achievement

    Science.gov (United States)

    Cano, Francisco; García, Ángela; Berbén, A. B. G.; Justicia, Fernando

    2014-07-01

    The purpose of this research was to build and test a conceptual model of the complex interrelationships between students' learning in science (learning approaches and self-regulation), their reading comprehension, question-asking in class and science achievement. These variables were measured by means of a test and a series of questionnaires administered to 604 ninth-grade students, and the data collected were analysed using a correlational, cross-sectional design. Results of a path analysis indicated that (a) students' self-regulated and intentional knowledge-constructing activity (self-regulated strategy use, deep approach and knowledge-building) were what chiefly accounted for their question-asking in class; (b) question-asking (high and low levels) was related directly to reading comprehension and indirectly, through its contribution to the this, to academic achievement; (c) reading comprehension was directly and negatively associated with surface approach and indirectly and positively related to deep approach and knowledge-building; and (d) some of these variables, particularly reading comprehension, accounted for academic achievement in science. This model explained nearly 30% of the variance in academic achievement and provided a substantial and distinctive insight into the web of interrelationships among these variables. Implications for future research and science teaching and learning are discussed (e.g. the importance of supporting students' efforts to learn science in a meaningful, active and self-regulated way and of improving their reading comprehension).

  19. In vitro inhibition of protease-activated receptors 1, 2 and 4 demonstrates that these receptors are not involved in an Acanthamoeba castellanii keratitis isolate-mediated disruption of the human brain microvascular endothelial cells.

    Science.gov (United States)

    Iqbal, Junaid; Naeem, Komal; Siddiqui, Ruqaiyyah; Khan, Naveed Ahmed

    2014-11-01

    Granulomatous amoebic encephalitis is a rare but serious human disease leading almost always to death. The pathophysiology of amoebic encephalitis is better understood, while events leading to the constitution of brain infection are largely unknown. Traversal of the blood-brain barrier is a key step in amoebae invasion of the central nervous system and facilitated by amoebic extracellular proteases. By using specific inhibitors of protease-activated receptors 1, 2 and 4, here we studied the role of these host receptors in Acanthamoeba castellanii-mediated damage to human brain microvasculature endothelial cells (HBMEC), which constitute the blood-brain barrier. The primary HBMEC were incubated with A. castellanii-conditioned medium in the presence or absence of FR-171113 (selective inhibitor of protease-activated receptor 1), FSLLRY-NH2 (inhibitor of protease-activated receptor 2), and tcY-NH2 (inhibitor of protease-activated receptor 4). The HBMEC monolayer disruptions were assessed by microscopy using Eosin staining, while host cell cytotoxicity was determined by measuring the release of cytoplasmic lactate dehydrogenase. Zymographic assays were performed to determine the effects of inhibitors of protease-activated receptors on the extracellular proteolytic activities of A. castellanii. A. castellanii-conditioned medium produced severe HBMEC monolayer disruptions within 60 min. The selective inhibitors of protease-activated receptors tested did not affect HBMEC monolayer disruptions. On the contrary, pre-treatment of A. castellanii-conditioned medium with phenylmethylsulfonyl fluoride, a serine protease inhibitor, or heating for 10 min at 95°C abolished HBMEC monolayer disruptions. Additionally, inhibitors of protease-activated receptors tested, failed to block A. castellanii-mediated HBMEC cytotoxicity and did not affect extracellular proteolytic activities of A. castellanii. Protease-activated receptors 1, 2 and 4 do not appear to play a role in A. castellanii

  20. Irvine Smart Grid Demonstration, a Regional Smart Grid Demonstration Project

    Energy Technology Data Exchange (ETDEWEB)

    Yinger, Robert [Southern California Edison Company, Rosemead, CA (United States); Irwin, Mark [Southern California Edison Company, Rosemead, CA (United States)

    2015-12-29

    ISGD was a comprehensive demonstration that spanned the electricity delivery system and extended into customer homes. The project used phasor measurement technology to enable substation-level situational awareness, and demonstrated SCE’s next-generation substation automation system. It extended beyond the substation to evaluate the latest generation of distribution automation technologies, including looped 12-kV distribution circuit topology using URCIs. The project team used DVVC capabilities to demonstrate CVR. In customer homes, the project evaluated HAN devices such as smart appliances, programmable communicating thermostats, and home energy management components. The homes were also equipped with energy storage, solar PV systems, and a number of energy efficiency measures (EEMs). The team used one block of homes to evaluate strategies and technologies for achieving ZNE. A home achieves ZNE when it produces at least as much renewable energy as the amount of energy it consumes annually. The project also assessed the impact of device-specific demand response (DR), as well as load management capabilities involving energy storage devices and plug-in electric vehicle charging equipment. In addition, the ISGD project sought to better understand the impact of ZNE homes on the electric grid. ISGD’s SENet enabled end-to-end interoperability between multiple vendors’ systems and devices, while also providing a level of cybersecurity that is essential to smart grid development and adoption across the nation. The ISGD project includes a series of sub-projects grouped into four logical technology domains: Smart Energy Customer Solutions, Next-Generation Distribution System, Interoperability and Cybersecurity, and Workforce of the Future. Section 2.3 provides a more detailed overview of these domains.

  1. The MAJORANA Demonstrator Radioassay Program

    Energy Technology Data Exchange (ETDEWEB)

    Abgrall, N.; Arnquist, Isaac J.; Avignone, F. T.; Back, Henning O.; Barabash, Alexander S.; Bertrand, F.; Boswell, M.; Bradley, A. W.; Brudanin, V.; Busch, Matthew; Buuck, M.; Byram, D.; Caldwell, A. S.; Chan, Yuen-Dat; Christofferson, C. D.; Chu, P. H.; Cuesta, C.; Detwiler, Jason A.; Dunmore, J. A.; Efremenko, Yuri; Ejiri, H.; Elliott, S.; Finnerty, P.; Galindo-Uribarri, A.; Gehman, Victor M.; Gilliss, T.; Giovanetti, G. K.; Goett, J.; Green, M. P.; Gruszko, J.; Guinn, I.; Guiseppe, V. E.; Henning, R.; Hoppe, Eric W.; Howard, S.; Howe, M. A.; Jasinski, B. R.; Johnson, R. A.; Keeter, K.; Kidd, M. F.; Kochetov, Oleg; Konovalov, S.; Kouzes, Richard T.; Laferriere, Brian D.; Leon, Jonathan D.; Loach, J. C.; MacMullin, J.; MacMullin, S.; Martin, R. D.; Massarcyk, R.; Meijer, S. J.; Mertens, S.; Miller, M. L.; Orrell, John L.; O' Shaughnessy, C.; Overman, Nicole R.; Poon, Alan W.; Pushkin, K.; Radford, D. C.; Rager, J.; Rielage, Keith; Robertson, R. G. H.; Romero-Romero, E.; Ronquest, M. C.; Schubert, Alexis G.; Shanks, B.; Shirchenko, M.; Snavely, Kyle J.; Snyder, N.; Steele, David; Suriano, Anne-Marie; Tedeschi, D.; Trimble, J. E.; Varner, R. L.; Vasilyev, Sergey; Vetter, K.; Vorren, Kris R.; White, B. R.; Wilkerson, J. F.; Wiseman, C.; Xu, W.; Yakushev, E.; Yu, Chang-Hong; Yumatov, Vladimir; Zhitnikov, I.

    2016-05-03

    The Majorana collaboration is constructing the Majorana Demonstrator at the Sanford Underground Research Facility at the Homestake gold mine, in Lead, SD. The apparatus will use Ge detectors, enriched in isotope 76Ge, to demonstrate the feasibility of a large-scale Ge detector experiment to search for neutrinoless double beta decay. The long half-life of this postulated process requires that the apparatus be extremely low in radioactive isotopes whose decays may produce backgrounds to the search. The radioassay program conducted by the collaboration to ensure that the materials comprising the apparatus are suffciently pure is described. The resulting measurements of the radioactiveisotope contamination for a number of materials studied for use in the detector are reported.

  2. Incineration demonstration at Savannah River

    International Nuclear Information System (INIS)

    Lewandowski, K.E.; Becker, G.W.; Mersman, K.E.; Roberson, W.A.

    1983-01-01

    A full-scale incineration process for Savannah River Plant (SRP) low level beta-gamma combustible waste was demonstrated at the Savannah River Laboratory (SRL) using nonradioactive wastes. From October 1981 through September 1982, 15,700 kilograms of solid waste and 5.7 m 3 of solvent were incinerated. Emissions of off-gas components (NO/sub x/, SO 2 , CO, and particulates) were well below South Carolina state standards. Volume reductions of 20:1 for solid waste and 7:1 for Purex solvent/lime slurry were achieved. Presently, the process is being upgraded by SRP to accept radioactive wastes. During a two-year SRP demonstration, the facility will be used to incinerate slightly radioactive ( 3 ) solvent and suspect level (<1 mR/hr at 0.0254 meter) solid wastes

  3. Plasma hearth process demonstration project

    International Nuclear Information System (INIS)

    Geimer, R.M.; Gillins, R.L.

    1995-01-01

    The Plasma Hearth Process (PHP) demonstration project is one of the key technology projects in the US Department of Energy (DOE) Office of Technology Development Mixed Waste Focus Area. The PHP is a high temperature thermal treatment process using a plasma arc torch in a stationary, refractory lined chamber that destroys organics and stabilizes the residuals in a nonleaching, vitrified waste form, greatly improving the disposability of the waste. This paper describes the PHP system and summarizes test results to date, including volume reduction, destruction and removal efficiencies for organic wastes, and emission characteristics. Tests performed so far demonstrate that the PHP adresses DOE mixed waste final waste form requirements and US Environmental Protection Agency Toxicity Characteristic Leaching Procedure requirements

  4. Electric thermal storage demonstration program

    Energy Technology Data Exchange (ETDEWEB)

    1992-02-01

    In early 1989, MMWEC, a joint action agency comprised of 30 municipal light departments in Massachusetts and on affiliate in Rhode Island, responded to a DOE request to proposal for the Least Cost Utility Planning program. The MMWEC submission was for the development of a program, focused on small rural electric utilities, to promote the use of electric thermal storage heating systems in residential applications. This report discusses the demonstration of ETS equipment at four member light departments.

  5. Electric thermal storage demonstration program

    Energy Technology Data Exchange (ETDEWEB)

    1992-01-01

    In early 1989, MMWEC, a joint action agency comprised of 30 municipal light departments in Massachusetts and on affiliate in Rhode Island, responded to a DOE request to proposal for the Least Cost Utility Planning program. The MMWEC submission was for the development of a program, focused on small rural electric utilities, to promote the use of electric thermal storage heating systems in residential applications. This report discusses the demonstration of ETS equipment at four member light departments.

  6. Solar heating demonstration. Final report

    Energy Technology Data Exchange (ETDEWEB)

    Bonicatto, L.; Kozak, C.

    1980-01-01

    The demonstration involved a 4-panel solar collector mounted on the industrial arts building. A 120 gallon storage tank supplements a 66 gallon electric hot water heater which supplies hot water for 5 shop wash basins, girl's and boy's lavatories, and a pressure washer in the auto shop. The installation and educational uses of the system are described. (MHR)

  7. Prototypical Consolidation Demonstration Project: Final report

    International Nuclear Information System (INIS)

    Gili, J.A.; Poston, V.K.

    1993-11-01

    This is the final report of the Prototypical Consolidation Demonstration Project, which was funded by the US Department of Energy's Office of Civilian Radioactive Waste Management. The project had two objectives: (a) to develop and demonstrate a prototype of production-scale equipment for the dry, horizontal consolidation and packaging of spent nuclear fuel rods from commercial boiling water reactor and pressurized water reactor fuel assemblies, and (b) to report the development and demonstration results to the US Department of Energy, Idaho Operations Office. This report summarizes the activities and conclusions of the project management contractor, EG ampersand G Idaho, Inc., and the fabrication and testing contractor, NUS Corporation (NUS). The report also presents EG ampersand G Idaho's assessments of the equipment and procedures developed by NUS

  8. Manufacturing Demonstration Facility: Low Temperature Materials Synthesis

    Energy Technology Data Exchange (ETDEWEB)

    Graham, David E. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Moon, Ji-Won [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Armstrong, Beth L. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Datskos, Panos G. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Duty, Chad E. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Gresback, Ryan [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Ivanov, Ilia N. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Jacobs, Christopher B. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Jellison, Gerald Earle [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Jang, Gyoung Gug [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Joshi, Pooran C. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Jung, Hyunsung [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Meyer, III, Harry M. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Phelps, Tommy [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

    2015-06-30

    The Manufacturing Demonstration Facility (MDF) low temperature materials synthesis project was established to demonstrate a scalable and sustainable process to produce nanoparticles (NPs) for advanced manufacturing. Previous methods to chemically synthesize NPs typically required expensive, high-purity inorganic chemical reagents, organic solvents and high temperatures. These processes were typically applied at small laboratory scales at yields sufficient for NP characterization, but insufficient to support roll-to-roll processing efforts or device fabrication. The new NanoFermentation processes described here operated at a low temperature (~60 C) in low-cost, aqueous media using bacteria that produce extracellular NPs with controlled size and elemental stoichiometry. Up-scaling activities successfully demonstrated high NP yields and quality in a 900-L pilot-scale reactor, establishing this NanoFermentation process as a competitive biomanufacturing strategy to produce NPs for advanced manufacturing of power electronics, solid-state lighting and sensors.

  9. Physics Teacher Demonstrations for the Classroom

    Science.gov (United States)

    Murfee, Lee

    2005-04-01

    A sharing of physics and physics teaching demonstrations by Lee Murfee, a teacher of students learning physics and mathematics at Berkeley Preparatory School and the United States Military Academy for 21 years, and active member of the Florida Section of American Association of Physics Teachers (AAPT). Presentation is a fast paced array of physics and physics teaching demonstrations. Topics include who and what we teach, a successful science department philosophy, forces, acceleration, impulse, momentum, observations, pendulums, springs, friction, inclined plane, rotational motion, moment of inertia, teaching description of motion with data, equations and graphing, slope, uniform circular motion, derivatives, integrals, PASCO Data Studio sensor applications, students presenting to students, flashboards, sound, pressure, and sensitivity analysis in determining specific heat. Demonstrations apply to high school and college introductory physics teaching; handouts and some door prizes/gifts will be provided.

  10. Cognitive training for children with ADHD: a randomized controlled trial of cogmed working memory training and 'paying attention in class'.

    Science.gov (United States)

    van der Donk, Marthe; Hiemstra-Beernink, Anne-Claire; Tjeenk-Kalff, Ariane; van der Leij, Aryan; Lindauer, Ramón

    2015-01-01

    The goal of this randomized controlled trial was to replicate and extend previous studies of Cogmed Working Memory Training (CWMT) in children with Attention-deficit/hyperactivity disorder (ADHD). While a large proportion of children with ADHD suffer from academic difficulties, only few previous efficacy studies have taken into account long term academic outcome measures. So far, results regarding academic outcome measures have been inconsistent. Hundred and two children with ADHD between the age of 8 and 12 years (both medicated and medication naïve) participated in current randomized controlled trial. Children were randomly assigned to CWMT or a new active combined working memory- and executive function compensatory training called 'Paying Attention in Class.' Primary outcome measures were neurocognitive functioning and academic performance. Secondary outcome measures contained ratings of behavior in class, behavior problems, and quality of life. Assessment took place before, directly after and 6 months after treatment. Results showed only one replicated treatment effect on visual spatial working memory in favor of CWMT. Effects of time were found for broad neurocognitive measures, supported by parent and teacher ratings. However, no treatment or time effects were found for the measures of academic performance, behavior in class or quality of life. We suggest that methodological and non-specific treatment factors should be taken into account when interpreting current findings. Future trials with well-blinded measures and a third 'no treatment' control group are needed before cognitive training can be supported as an evidence-based treatment of ADHD. Future research should put more effort into investigating why, how and for whom cognitive training is effective as this would also potentially lead to improved intervention- and study designs.

  11. Phenolphthalein—Pink Tornado Demonstration

    Science.gov (United States)

    Prall, Bruce R.

    2008-04-01

    The phenolphthalein-pink tornado demonstration utilizes the vortex generated by a spinning magnetic stirring bar in a 1 L graduated cylinder containing 0.01 M HCl to demonstrate Le Châtelier's principle as it applies to the phenolphthalein equilibrium in water H 2 In + 2H 2 O 2H 2 O + + In 2 - where H 2 In is phenophthalein. The addition of 3-4 drops of phenolphthalein indicator solution followed immediately by 3-4 drops of 50% (w/w) NaOH to the vortex of the HCl solution results in a shift to the right in the equilibrium owing to the reaction of OH - + H 3 O + to form water. This shift is accompanied by the vortex becoming visible by the appearance of a pinkish-red color caused by an increase in In 2- concentration within the localized region of the vortex. The demonstration also provides one an excellent opportunity to discuss the topics of limiting reagent and reagent in excess. Some insight regarding the extent to which uniform mixing is achieved when using a magnetic stirrer is also provided. Included is a note from the Feature Editor, Ed Vitz.

  12. Infiltration barrier demonstration at Maxey Flats, Kentucky

    International Nuclear Information System (INIS)

    Mills, D.; Razor, J.

    1983-01-01

    At the 1982 DOE LLWMP meeting, the Kentucky Natural Resources and Environmental Protection Cabinet presented a history of the Maxey Flats Waste Disposal Facility, its status, and current Commonwealth activities leading toward stabilization and decommissioning. Information was presented at that time on the purpose of the DOE Trench Moisture Barrier Demonstration Grant and the early phases of construction and implementation. In this paper, final construction and implementation of the trench moisture barrier demonstration are discussed. Data including trench water level measurements, lateral liquid flow in experimental sections, and soil moisture measurements are presented and discussed. The Paper is completed with a brief discussion of remediation activities currently being implemented at Maxey Flats. 9 references, 7 figures, 1 table

  13. Final report for the cryogenic retrieval demonstration

    International Nuclear Information System (INIS)

    Valentich, D.J.; Yokuda, E.L.

    1992-09-01

    This report documents a demonstration of a proposed buried transuranic waste retrieval concept that uses cryogenic ground freezing and remote excavation. At the Idaho National Engineering Laboratory (INEL), there are over 8 million ft 3 of intermingled soil and transuranic (TRU) wastes in shallow land burial, and retrieval of the material is one of the options being considered by the Buried Waste Integrated Demonstration for the Environmental Restoration program. Cryogenically freezing contaminated soil and buried waste has been proposed as a way to greatly reduce or eliminate the climate the threat of contamination spread during retrieval activities. In support of this idea, a demonstration of an innovative ground freezing and retrieval technology was performed at the INEL. This initial demonstration was held near the Radioactive Waste Management Complex at a ''cold test pit'' that was built in 1988 as a test bed for the demonstration of retrieval contamination control technologies. This pit is not contaminated with any radioactive or hazardous wastes. Barrels and boxes filled with metals, plastics, tools, paper, cloth, etc. configured in the same manner as expected in contaminated pits and trenches are buried at the cold test pit. After design, fabrication, and shop testing, Sonsub mobilized to the field in early July 1992 to perform the field demonstration. It was planned to freeze and extract four pits, each 9 x 9 x 10 ft. Each pit represented a different configuration of buried waste (stacked boxes, stacked barrels, random dumped barrels and boxes, and random dumped barrels). Sonsub's proposed technology consisted of driving a series of freeze pipes into the soil and waste, using liquid nitrogen to freeze the mass, and extracting the soil and debris using a series of remote operated, bridge crane mounted tools. In conjunction with the freezing and removal activities, temperature and moisture measurements, and air monitoring were performed

  14. Final report for the cryogenic retrieval demonstration

    Energy Technology Data Exchange (ETDEWEB)

    Valentich, D.J.; Yokuda, E.L.

    1992-09-01

    This report documents a demonstration of a proposed buried transuranic waste retrieval concept that uses cryogenic ground freezing and remote excavation. At the Idaho National Engineering Laboratory (INEL), there are over 8 million ft{sup 3} of intermingled soil and transuranic (TRU) wastes in shallow land burial, and retrieval of the material is one of the options being considered by the Buried Waste Integrated Demonstration for the Environmental Restoration program. Cryogenically freezing contaminated soil and buried waste has been proposed as a way to greatly reduce or eliminate the climate the threat of contamination spread during retrieval activities. In support of this idea, a demonstration of an innovative ground freezing and retrieval technology was performed at the INEL. This initial demonstration was held near the Radioactive Waste Management Complex at a ``cold test pit`` that was built in 1988 as a test bed for the demonstration of retrieval contamination control technologies. This pit is not contaminated with any radioactive or hazardous wastes. Barrels and boxes filled with metals, plastics, tools, paper, cloth, etc. configured in the same manner as expected in contaminated pits and trenches are buried at the cold test pit. After design, fabrication, and shop testing, Sonsub mobilized to the field in early July 1992 to perform the field demonstration. It was planned to freeze and extract four pits, each 9 {times} 9 {times} 10 ft. Each pit represented a different configuration of buried waste (stacked boxes, stacked barrels, random dumped barrels and boxes, and random dumped barrels). Sonsub`s proposed technology consisted of driving a series of freeze pipes into the soil and waste, using liquid nitrogen to freeze the mass, and extracting the soil and debris using a series of remote operated, bridge crane mounted tools. In conjunction with the freezing and removal activities, temperature and moisture measurements, and air monitoring were performed.

  15. Final report for the cryogenic retrieval demonstration

    Energy Technology Data Exchange (ETDEWEB)

    Valentich, D.J.; Yokuda, E.L.

    1992-09-01

    This report documents a demonstration of a proposed buried transuranic waste retrieval concept that uses cryogenic ground freezing and remote excavation. At the Idaho National Engineering Laboratory (INEL), there are over 8 million ft[sup 3] of intermingled soil and transuranic (TRU) wastes in shallow land burial, and retrieval of the material is one of the options being considered by the Buried Waste Integrated Demonstration for the Environmental Restoration program. Cryogenically freezing contaminated soil and buried waste has been proposed as a way to greatly reduce or eliminate the climate the threat of contamination spread during retrieval activities. In support of this idea, a demonstration of an innovative ground freezing and retrieval technology was performed at the INEL. This initial demonstration was held near the Radioactive Waste Management Complex at a cold test pit'' that was built in 1988 as a test bed for the demonstration of retrieval contamination control technologies. This pit is not contaminated with any radioactive or hazardous wastes. Barrels and boxes filled with metals, plastics, tools, paper, cloth, etc. configured in the same manner as expected in contaminated pits and trenches are buried at the cold test pit. After design, fabrication, and shop testing, Sonsub mobilized to the field in early July 1992 to perform the field demonstration. It was planned to freeze and extract four pits, each 9 [times] 9 [times] 10 ft. Each pit represented a different configuration of buried waste (stacked boxes, stacked barrels, random dumped barrels and boxes, and random dumped barrels). Sonsub's proposed technology consisted of driving a series of freeze pipes into the soil and waste, using liquid nitrogen to freeze the mass, and extracting the soil and debris using a series of remote operated, bridge crane mounted tools. In conjunction with the freezing and removal activities, temperature and moisture measurements, and air monitoring were

  16. Environmental analysis for pipeline gas demonstration plants

    Energy Technology Data Exchange (ETDEWEB)

    Stinton, L.H.

    1978-09-01

    The Department of Energy (DOE) has implemented programs for encouraging the development and commercialization of coal-related technologies, which include coal gasification demonstration-scale activities. In support of commercialization activities the Environmental Analysis for Pipeline Gas Demonstration Plants has been prepared as a reference document to be used in evaluating potential environmental and socioeconomic effects from construction and operation of site- and process-specific projects. Effluents and associated impacts are identified for six coal gasification processes at three contrasting settings. In general, impacts from construction of a high-Btu gas demonstration plant are similar to those caused by the construction of any chemical plant of similar size. The operation of a high-Btu gas demonstration plant, however, has several unique aspects that differentiate it from other chemical plants. Offsite development (surface mining) and disposal of large quantities of waste solids constitute important sources of potential impact. In addition, air emissions require monitoring for trace metals, polycyclic aromatic hydrocarbons, phenols, and other emissions. Potential biological impacts from long-term exposure to these emissions are unknown, and additional research and data analysis may be necessary to determine such effects. Possible effects of pollutants on vegetation and human populations are discussed. The occurrence of chemical contaminants in liquid effluents and the bioaccumulation of these contaminants in aquatic organisms may lead to adverse ecological impact. Socioeconomic impacts are similar to those from a chemical plant of equivalent size and are summarized and contrasted for the three surrogate sites.

  17. MYC/BCL2 protein coexpression contributes to the inferior survival of activated B-cell subtype of diffuse large B-cell lymphoma and demonstrates high-risk gene expression signatures

    DEFF Research Database (Denmark)

    Hu, Shimin; Xu-Monette, Zijun Y; Tzankov, Alexander

    2013-01-01

    Diffuse large B-cell lymphoma (DLBCL) is stratified into prognostically favorable germinal center B-cell (GCB)-like and unfavorable activated B-cell (ABC)-like subtypes based on gene expression signatures. In this study, we analyzed 893 de novo DLBCL patients treated with R-CHOP (rituximab...

  18. Virginia Demonstration Project Encouraging Middle School Students in Pursuing STEM Careers

    Science.gov (United States)

    Bachman, Jane T.; Kota, Dena H.; Kota, Aaron J.

    2011-01-01

    Encouraging students at all grade levels to consider pursuing a career in Science, Technology, Engineering, and Mathematics (STEM) fields i s a national focus. In 2005, the Naval Surface Warfare Center, Dahlgren Division (NSWCDD), a Department of Defense laboratory located in Da hlgren, Virginia, began work on the Virginia Demonstration Project (VDP) with the goal of increasing more student interest in STEM educatio n and pursuing STEM careers. This goal continues as the program enters its sixth year. This project has been successful through the partici pation of NSWCDD's scientists and engineers who are trained as mentor s to work in local middle school classrooms throughout the school year, As an extension of the in-class activities, several STEM summer aca demies have been conducted at NSWCDD, These academies are supported by the Navy through the VDP and the STEM Learning Module Project. These projects are part of more extensive outreach efforts offered by the National Defense Education Program (NDEP), sponsored by the Director, Defense Research and Engineering. The focus of this paper is on the types of activities conducted at the summer academy, an overview of the academy planning process, and recommendations to help support a nati onal plan of integrating modeling and simulation-based engineering and science into all grade levels. based upon the lessons learned

  19. The Edgewater Coolside process demonstration

    Energy Technology Data Exchange (ETDEWEB)

    McCoy, D.C.; Scandrol, R.O.; Statnick, R.M.; Stouffer, M.R.; Winschel, R.A.; Withum, J.A.; Wu, M.M.; Yoon, H. (CONSOL, Inc., Pittsburgh, PA (United States))

    1992-02-01

    The Edgewater Coolside process demonstration met the program objectives which were to determine Coolside SO[sub 2] removal performance, establish short-term process operability, and evaluate the economics of the process versus a limestone wet scrubber. On a flue gas produced from the combustion of 3% sulfur coal, the Coolside process achieved 70% SO[sub 2] removal using commercially-available hydrated lime as the sorbent. The operating conditions were Ca/S mol ratio 2.0, Na/Ca mol ratio 0.2, and 20[degree]F approach to adiabatic saturation temperature ([del]T). During tests using fresh plus recycle sorbent, the recycle sorbent exhibited significant capacity for additional SO[sub 2] removal. The longest steady state operation was eleven days at nominally Ca/S = 2, Na/Ca = 0.22, [del]T = 20--22[degree]F, and 70% SO[sub 2] removal. The operability results achieved during the demonstration indicate that with the recommended process modifications, which are discussed in the Coolside process economic analysis, the process could be designed as a reliable system for utility application. Based on the demonstration program, the Coolside process capital cost for a hypothetical commercial installation was minimized. The optimization consisted of a single, large humidifier, no spare air compressor, no isolation dampers, and a 15 day on-site hydrated lime storage. The levelized costs of the Coolside and the wet limestone scrubbing processes were compared. The Coolside process is generally economically competitive with wet scrubbing for coals containing up to 2.5% sulfur and plants under 350 MWe. Site-specific factors such as plant capacity factor, SO[sub 2] emission limit, remaining plant life, retrofit difficulty, and delivered sorbent cost affect the scrubber-Coolside process economic comparison.

  20. The Edgewater Coolside process demonstration

    Energy Technology Data Exchange (ETDEWEB)

    McCoy, D.C.; Scandrol, R.O.; Statnick, R.M.; Stouffer, M.R.; Winschel, R.A.; Withum, J.A.; Wu, M.M.; Yoon, H. [CONSOL, Inc., Pittsburgh, PA (United States)

    1992-02-01

    The Edgewater Coolside process demonstration met the program objectives which were to determine Coolside SO{sub 2} removal performance, establish short-term process operability, and evaluate the economics of the process versus a limestone wet scrubber. On a flue gas produced from the combustion of 3% sulfur coal, the Coolside process achieved 70% SO{sub 2} removal using commercially-available hydrated lime as the sorbent. The operating conditions were Ca/S mol ratio 2.0, Na/Ca mol ratio 0.2, and 20{degree}F approach to adiabatic saturation temperature ({del}T). During tests using fresh plus recycle sorbent, the recycle sorbent exhibited significant capacity for additional SO{sub 2} removal. The longest steady state operation was eleven days at nominally Ca/S = 2, Na/Ca = 0.22, {del}T = 20--22{degree}F, and 70% SO{sub 2} removal. The operability results achieved during the demonstration indicate that with the recommended process modifications, which are discussed in the Coolside process economic analysis, the process could be designed as a reliable system for utility application. Based on the demonstration program, the Coolside process capital cost for a hypothetical commercial installation was minimized. The optimization consisted of a single, large humidifier, no spare air compressor, no isolation dampers, and a 15 day on-site hydrated lime storage. The levelized costs of the Coolside and the wet limestone scrubbing processes were compared. The Coolside process is generally economically competitive with wet scrubbing for coals containing up to 2.5% sulfur and plants under 350 MWe. Site-specific factors such as plant capacity factor, SO{sub 2} emission limit, remaining plant life, retrofit difficulty, and delivered sorbent cost affect the scrubber-Coolside process economic comparison.

  1. Dynamic Underground Stripping Demonstration Project

    International Nuclear Information System (INIS)

    Aines, R.; Newmark, R.; McConachie, W.; Rice, D.; Ramirez, A.; Siegel, W.; Buettner, M.; Daily, W.; Krauter, P.; Folsom, E.; Boegel, A.J.; Bishop, D.; udel, K.

    1992-03-01

    LLNL is collaborating with the UC Berkeley College of Engineering to develop and demonstrate a system of thermal remediation and underground imaging techniques for use in rapid cleanup of localized underground spills. Called ''Dynamic Stripping'' to reflect the rapid and controllable nature of the process, it will combine steam injection, direct electrical heating, and tomographic geophysical imaging in a cleanup of the LLNL gasoline spill. In the first 8 months of the project, a Clean Site engineering test was conducted to prove the field application of the techniques before moving to the contaminated site in FY 92

  2. Aeroflex Technology as Class-Y Demonstrator

    Science.gov (United States)

    Suh, Jong-ook; Agarwal, Shri; Popelar, Scott

    2014-01-01

    Modern space field programmable gate array (FPGA) devices with increased functional density and operational frequency, such as Xilinx Virtex 4 (V4) and S (V5), are packaged in non-hermetic ceramic flip chip forms. These next generation space parts were not qualified to the MIL-PRF-38535 Qualified Manufacturer Listing (QML) class-V when they were released because class-V was only intended for hermetic parts. In order to bring Xilinx V5 type packages into the QML system, it was suggested that class-Y be set up as a new category. From 2010 through 2014, a JEDEC G12 task group developed screening and qualification requirements for Class-Y products. The Document Standardization Division of the Defense Logistics Agency (DLA) has completed an engineering practice study. In parallel with the class-Y efforts, the NASA Electronic Parts and Packaging (NEPP) program has funded JPL to study potential reliability issues of the class-Y products. The major hurdle of this task was the absence of adequate research samples. Figure 1-1 shows schematic diagrams of typical structures of class-Y type products. Typically, class-Y products are either in ceramic flip chip column grid array (CGA) or land grid array (LGA) form. In class-Y packages, underfill and heat spread adhesive materials are directly exposed to the spacecraft environment due to their non-hermeticity. One of the concerns originally raised was that the underfill material could degrade due to the spacecraft environment and negatively impact the reliability of the package. In order to study such issues, it was necessary to use ceramic daisy chain flip chip package samples so that continuity of flip chip solder bumps could be monitored during the reliability tests. However, none of the commercially available class-Y daisy chain parts had electrical connections through flip chip solder bumps; only solder columns were daisy chained, which made it impossible to test continuity of flip chip solder bumps without using extremely

  3. Bentonite mat demonstration. Final report

    Energy Technology Data Exchange (ETDEWEB)

    Serrato, M.G.

    1994-12-30

    The Bentonite Mat Demonstration was developed to provide the Environmental Restoration Department with field performance characteristics and engineering data for an alternative closure cover system configuration. The demonstration was initiated in response to regulatory concerns regarding the use of an alternative cover system for future design configurations. These design considerations are in lieu of the US Environmental Protection Agency (EPA) Recommended Design for Closure Cover Systems and specifically a single compacted kaolin clay layer with a hydraulic conductivity of 1 {times} 10{sup {minus}7} cm/sec. This alternative configuration is a composite geosynthetic material hydraulic barrier consisting from bottom to top: 2 ft compacted sandy clay layer (typical local Savannah River Site soil type) that is covered by a bentonite mat--geosynthetic clay liner (GCL) and is overlaid by a 40 mil High Density Polyethylene (HDPE) geomembrane--flexible membrane liner. This effort was undertaken to obtain and document the necessary field performance/engineering data for future designs and meet regulatory technical requirements for an alternative cover system configuration. The composite geosynthetic materials hydraulic barrier is the recommended alternative cover system configuration for containment of hazardous and low level radiological waste layers that have a high potential of subsidence to be used at the Savannah River Site (SRS). This alternative configuration mitigates subsidence effects in providing a flexible, lightweight cover system to maintain the integrity of the closure. The composite geosynthetic materials hydraulic barrier is recommended for the Sanitary Landfill and Low Level Radiological Waste Disposal Facility (LLRWDF) Closures.

  4. Parker Hybrid Hydraulic Drivetrain Demonstration

    Energy Technology Data Exchange (ETDEWEB)

    Collett, Raymond [Parker-Hannifin Corporation, Cleveland, OH (United States); Howland, James [Parker-Hannifin Corporation, Cleveland, OH (United States); Venkiteswaran, Prasad [National Energy Technology Lab. (NETL), Morgantown, WV (United States)

    2014-03-31

    This report examines the benefits of Parker Hannifin hydraulic hybrid brake energy recovery systems used in commercial applications for vocational purposes. A detailed background on the problem statement being addressed as well as the solution set specific for parcel delivery will be provided. Objectives of the demonstration performed in high start & stop applications included opportunities in fuel usage reduction, emissions reduction, vehicle productivity, and vehicle maintenance. Completed findings during the demonstration period and parallel investigations with NREL, CALSTART, along with a literature review will be provided herein on this research area. Lastly, results identified in the study by third parties validated the savings potential in fuel reduction of on average of 19% to 52% over the baseline in terms of mpg (Lammert, 2014, p11), Parker data for parcel delivery vehicles in the field parallels this at a range of 35% - 50%, emissions reduction of 17.4% lower CO2 per mile and 30.4% lower NOx per mile (Gallo, 2014, p15), with maintenance improvement in the areas of brake and starter replacement, while leaving room for further study in the area of productivity in terms of specific metrics that can be applied and studied.

  5. The ATLAS IBL BOC Demonstrator

    CERN Document Server

    Ancu, J; The ATLAS collaboration; Falchieri, D; Flick, T; Gabrielli, A; Grosse-Knetter, J; Heim, T; Joseph, J; Krieger, N; Kugel, A; Morettini, P; Neumann, M; Polini, A; Schneider, B; Schroer, N

    2011-01-01

    The Insertable-B-Layer (IBL) is a new pixel detector layer to be installed at the ATLAS experiment at the LHC, CERN in 2013. It will be integrated into the general pixel readout and software framework, hence the off-detector readout electronics has to support the new front-end electronics whilst maintaining a high degree of interoperability to the components of the existing system. The off-detector readout is realised using a number of VME card pairs – ROD and BOC – plus a VME crate controller and a custom timing distribution system. The main elements of the new BOC design comprise optical interfaces towards the detector, signal conditioning and data recovery logic. We present the demonstrator used to verify the design approach. The demonstrator is based on a XILINX SP605 FPGA evaluation board and uses a Microblaze processor inside the FPGA to provide easy and flexible access to all essential BOC functions and the corresponding emulator modules, which enable full test of the entire BOC functionality even ...

  6. Vehicle to Grid Demonstration Project

    Energy Technology Data Exchange (ETDEWEB)

    Willett Kempton; Meryl Gardner; Michael Hidrue; Fouad Kamilev; Sachin Kamboj; Jon Lilley; Rodney McGee; George Parsons; Nat Pearre; Keith Trnka

    2010-12-31

    This report summarizes the activities and accomplishments of a two-year DOE-funded project on Grid-Integrated Vehicles (GIV) with vehicle to grid power (V2G). The project included several research and development components: an analysis of US driving patterns; an analysis of the market for EVs and V2G-capable EVs; development and testing of GIV components (in-car and in-EVSE); interconnect law and policy; and development and filing of patents. In addition, development activities included GIV manufacturing and licensing of technologies developed under this grant. Also, five vehicles were built and deployed, four for the fleet of the State of Delaware, plus one for the University of Delaware fleet.

  7. Energy 2007. Research, development, demonstration; Energi 07. Forskning, udvikling, demonstration

    Energy Technology Data Exchange (ETDEWEB)

    Byriel, I.P.; Justesen, Helle; Beck, A.; Borup Jensen, J.; Rosenfeldt Jakobsen, Kl; Jacobsen, Steen Hartvig (eds.)

    2007-08-10

    Danish energy research is in an exciting and challenging situation. Rising oil prices, unstable energy supply, climate policy responsibilities and globalization have brought development of new environmentally friendly and more efficient energy technologies into focus. Promising international markets for newly developed energy technologies are emerging, and at the same time well established Danish positions of strength are challenged by new strong actors on the global market. The Danish government has set to work on its vision of an appreciable strengthening of public energy research funding through the recent law on the energy technological development and demonstration programme EUDP and the realization of globalization funds. The interaction between basic and applied research must be kept intact. In this report the various Danish energy research programmes administered by Energinet.dk, Danish Energy Authority, Danish Energy Association, Danish Council for Strategic Research's Programme Commission on Energy and Environment and Danish National Advanced Technology Foundation, coordinate their annual reports for the first time. The aim of Energy 2007 is to give the reader an idea of how the energy research programmes collaborate on solving the major energy technology challenges - also in an international context. (BA)

  8. Deep Space Habitat Concept Demonstrator

    Science.gov (United States)

    Bookout, Paul S.; Smitherman, David

    2015-01-01

    This project will develop, integrate, test, and evaluate Habitation Systems that will be utilized as technology testbeds and will advance NASA's understanding of alternative deep space mission architectures, requirements, and operations concepts. Rapid prototyping and existing hardware will be utilized to develop full-scale habitat demonstrators. FY 2014 focused on the development of a large volume Space Launch System (SLS) class habitat (Skylab Gen 2) based on the SLS hydrogen tank components. Similar to the original Skylab, a tank section of the SLS rocket can be outfitted with a deep space habitat configuration and launched as a payload on an SLS rocket. This concept can be used to support extended stay at the Lunar Distant Retrograde Orbit to support the Asteroid Retrieval Mission and provide a habitat suitable for human missions to Mars.

  9. Navy fuel cell demonstration project.

    Energy Technology Data Exchange (ETDEWEB)

    Black, Billy D.; Akhil, Abbas Ali

    2008-08-01

    This is the final report on a field evaluation by the Department of the Navy of twenty 5-kW PEM fuel cells carried out during 2004 and 2005 at five Navy sites located in New York, California, and Hawaii. The key objective of the effort was to obtain an engineering assessment of their military applications. Particular issues of interest were fuel cell cost, performance, reliability, and the readiness of commercial fuel cells for use as a standalone (grid-independent) power option. Two corollary objectives of the demonstration were to promote technological advances and to improve fuel performance and reliability. From a cost perspective, the capital cost of PEM fuel cells at this stage of their development is high compared to other power generation technologies. Sandia National Laboratories technical recommendation to the Navy is to remain involved in evaluating successive generations of this technology, particularly in locations with greater environmental extremes, and it encourages their increased use by the Navy.

  10. Prototypical Rod Consolidation Demonstration Project

    International Nuclear Information System (INIS)

    1993-05-01

    The objective of Phase 3 of the Prototypical Rod consolidation Demonstration Project (PRCDP) was to procure, fabricate, assemble, and test the Prototypical Rod consolidation System as described in the NUS Phase 2 Final Design Report. This effort required providing the materials, components, and fabricated parts which makes up all of the system equipment. In addition, it included the assembly, installation, and setup of this equipment at the Cold Test Facility. During the Phase 3 effort the system was tested on a component, subsystem, and system level. This volume 1, discusses the PRCDP Phase 3 Test Program that was conducted by the HALLIBURTON NUS Environmental Corporation under contract AC07-86ID12651 with the United States Department of Energy. This document, Volume 1, Book 1 discusses the following topics: the background of the project; test program description; summary of tests and test results; problem evaluation; functional requirements confirmation; recommendations; and completed test documentation for tests performed in Phase 3

  11. Prototypical Rod Consolidation Demonstration Project

    International Nuclear Information System (INIS)

    1993-05-01

    The objective of Phase 3 of the Prototypical Rod consolidation Demonstration Project (PRCDP) was to procure, fabricate, assemble, and test the Prototypical Rod consolidation System as described in the NUS Phase 2 Final Design Report. This effort required providing the materials, components, and fabricated parts which makes up all of the system equipment. In addition, it included the assembly, installation, and setup of this equipment at the Cold Test Facility. During the Phase 3 effort the system was tested on a component, subsystem, and system level. This volume 1, discusses the PRCDP Phase 3 Test Program that was conducted by the HALLIBURTON NUS Environmental Corporation under contract AC07-86ID12651 with the United States Department of Energy. This document, Volume 1, Book 9 discusses the following topics: Integrated System Normal Operations Test Results and Analysis Report; Integrated System Off-Normal Operations Test Results and Analysis Report; and Integrated System Maintenance Operations Test Results and Analysis Report

  12. The Liquid Argon Purity Demonstrator

    Energy Technology Data Exchange (ETDEWEB)

    Adamowski, M.; Carls, B.; Dvorak, E.; Hahn, A.; Jaskierny, W.; Johnson, C.; Jostlein, H.; Kendziora, C.; Lockwitz, S.; Pahlka, B.; Plunkett, R.; Pordes, S.; Rebel, B.; Schmitt, R.; Stancari, M.; Tope, T.; Voirin, E.; Yang, T.

    2014-07-01

    The Liquid Argon Purity Demonstrator was an R&D test stand designed to determine if electron drift lifetimes adequate for large neutrino detectors could be achieved without first evacuating the cryostat. We describe here the cryogenic system, its operations, and the apparatus used to determine the contaminant levels in the argon and to measure the electron drift lifetime. The liquid purity obtained by this system was facilitated by a gaseous argon purge. Additionally, gaseous impurities from the ullage were prevented from entering the liquid at the gas-liquid interface by condensing the gas and filtering the resulting liquid before returning to the cryostat. The measured electron drift lifetime in this test was greater than 6 ms, sustained over several periods of many weeks. Measurements of the temperature profile in the argon, to assess convective flow and boiling, were also made and are compared to simulation.

  13. Pilot Scale Advanced Fogging Demonstration

    Energy Technology Data Exchange (ETDEWEB)

    Demmer, Rick L. [Idaho National Lab. (INL), Idaho Falls, ID (United States); Fox, Don T. [Idaho National Lab. (INL), Idaho Falls, ID (United States); Archiblad, Kip E. [Idaho National Lab. (INL), Idaho Falls, ID (United States)

    2015-01-01

    Experiments in 2006 developed a useful fog solution using three different chemical constituents. Optimization of the fog recipe and use of commercially available equipment were identified as needs that had not been addressed. During 2012 development work it was noted that low concentrations of the components hampered coverage and drying in the United Kingdom’s National Nuclear Laboratory’s testing much more so than was evident in the 2006 tests. In fiscal year 2014 the Idaho National Laboratory undertook a systematic optimization of the fogging formulation and conducted a non-radioactive, pilot scale demonstration using commercially available fogging equipment. While not as sophisticated as the equipment used in earlier testing, the new approach is much less expensive and readily available for smaller scale operations. Pilot scale testing was important to validate new equipment of an appropriate scale, optimize the chemistry of the fogging solution, and to realize the conceptual approach.

  14. Prototypical Rod Consolidation Demonstration Project

    International Nuclear Information System (INIS)

    1993-05-01

    The objective of Phase 3 of the Prototypical Rod consolidation Demonstration Project (PRCDP) was to procure, fabricate, assemble, and test the Prototypical Rod consolidation System as described in the NUS Phase 2 Final Design Report. This effort required providing the materials, components, and fabricated parts which makes up all of the system equipment. In addition, it included the assembly, installation, and setup of this equipment at the Cold Test Facility. During the Phase 3 effort the system was tested on a component, subsystem, and system level. This volume 1, discusses the PRCDP Phase 3 Test Program that was conducted by the HALLIBURTON NUS Environmental Corporation under contract AC07-86ID12651 with the United States Department of Energy. This document, Volume 1, Book 2 discusses the following topics: Fuel Rod Extraction System Test Results and Analysis Reports and Clamping Table Test Results and Analysis Reports

  15. Clean Coal Diesel Demonstration Project

    Energy Technology Data Exchange (ETDEWEB)

    Robert Wilson

    2006-10-31

    A Clean Coal Diesel project was undertaken to demonstrate a new Clean Coal Technology that offers technical, economic and environmental advantages over conventional power generating methods. This innovative technology (developed to the prototype stage in an earlier DOE project completed in 1992) enables utilization of pre-processed clean coal fuel in large-bore, medium-speed, diesel engines. The diesel engines are conventional modern engines in many respects, except they are specially fitted with hardened parts to be compatible with the traces of abrasive ash in the coal-slurry fuel. Industrial and Municipal power generating applications in the 10 to 100 megawatt size range are the target applications. There are hundreds of such reciprocating engine power-plants operating throughout the world today on natural gas and/or heavy fuel oil.

  16. Demonstration of creep during filtration

    DEFF Research Database (Denmark)

    Christensen, Morten Lykkegaard; Bugge, Thomas Vistisen; Kirchheiner, Anders Løvenbalk

    that the production of filtrate also depends on the characteristic time for the filter cake solids to deform. This is formulated in the Terzaghi-Voigt model in which a secondary consolidation is introduced. The secondary consolidation may be visualized by plots of the relative cake deformation (U) v.s. the square...... root of time. Even more clearly it is demonstrated by plotting the liquid pressure at the cake piston interface v.s. the relative deformation (to be shown). The phenomenon of a secondary consolidation processes is in short called creep. Provided that the secondary consolidation rate is of the same......The classical filtration theory assumes a unique relationship between the local filter cake porosity and the local effective pressure. For a number of compressible materials, it has however been observed that during the consolidation stage this may not be the case. It has been found...

  17. Innovative grout/retrieval demonstration final report

    Energy Technology Data Exchange (ETDEWEB)

    Loomis, G.G.; Thompson, D.N.

    1995-01-01

    This report presents the results of an evaluation of an innovative retrieval technique for buried transuranic waste. Application of this retrieval technique was originally designed for full pit retrieval; however, it applies equally to a hot spot retrieval technology. The technique involves grouting the buried soil waste matrix with a jet grouting procedure, applying an expansive demolition grout to the matrix, and retrieving the debris. The grouted matrix provides an agglomeration of fine soil particles and contaminants resulting in an inherent contamination control during the dusty retrieval process. A full-scale field demonstration of this retrieval technique was performed on a simulated waste pit at the Idaho National Engineering Laboratory. Details are reported on all phases of this proof-of-concept demonstration including pit construction, jet grouting activities, application of the demolition grout, and actual retrieval of the grouted pit. A quantitative evaluation of aerosolized soils and rare earth tracer spread is given for all phases of the demonstration, and these results are compared to a baseline retrieval activity using conventional retrieval means. 8 refs., 47 figs., 10 tabs.

  18. Innovative grout/retrieval demonstration final report

    International Nuclear Information System (INIS)

    Loomis, G.G.; Thompson, D.N.

    1995-01-01

    This report presents the results of an evaluation of an innovative retrieval technique for buried transuranic waste. Application of this retrieval technique was originally designed for full pit retrieval; however, it applies equally to a hot spot retrieval technology. The technique involves grouting the buried soil waste matrix with a jet grouting procedure, applying an expansive demolition grout to the matrix, and retrieving the debris. The grouted matrix provides an agglomeration of fine soil particles and contaminants resulting in an inherent contamination control during the dusty retrieval process. A full-scale field demonstration of this retrieval technique was performed on a simulated waste pit at the Idaho National Engineering Laboratory. Details are reported on all phases of this proof-of-concept demonstration including pit construction, jet grouting activities, application of the demolition grout, and actual retrieval of the grouted pit. A quantitative evaluation of aerosolized soils and rare earth tracer spread is given for all phases of the demonstration, and these results are compared to a baseline retrieval activity using conventional retrieval means. 8 refs., 47 figs., 10 tabs

  19. Comparative Functional Alanine Positional Scanning of the α-Melanocyte Stimulating Hormone and NDP-Melanocyte Stimulating Hormone Demonstrates Differential Structure-Activity Relationships at the Mouse Melanocortin Receptors.

    Science.gov (United States)

    Todorovic, Aleksandar; Ericson, Mark D; Palusak, Ryan D; Sorensen, Nicholas B; Wood, Michael S; Xiang, Zhimin; Haskell-Luevano, Carrie

    2016-07-20

    The melanocortin system has been implicated in the regulation of various physiological functions including melanogenesis, steroidogenesis, energy homeostasis, and feeding behavior. Five melanocortin receptors have been identified to date and belong to the family of G protein-coupled receptors (GPCR). Post-translational modification of the proopiomelanocortin (POMC) prohormone leads to the biosynthesis of the endogenous melanocortin agonists, including α-melanocyte stimulating hormone (α-MSH), β-MSH, γ-MSH, and adrenocorticotropic hormone (ACTH). All the melanocortin agonists derived from the POMC prohormone contain a His-Phe-Arg-Trp tetrapeptide sequence that has been implicated in eliciting the pharmacological responses at the melanocortin receptors. Herein, an alanine (Ala) positional scan is reported for the endogenous α-MSH ligand and the synthetic, more potent, NDP-MSH peptide (Ac-Ser(1)-Tyr(2)-Ser(3)-Nle(4)-Glu(5)-His(6)-DPhe(7)-Arg(8)-Trp(9)-Gly(10)-Lys(11)-Pro(12)-Val(13)-NH2) at the cloned mouse melanocortin receptors to test the assumption that the structure-activity relationships of one ligand would apply to the other. Several residues outside of the postulated pharmacophore altered potency at the melanocortin receptors, most notably the 1560-, 37-, and 15-fold potency loss when the Glu(5) position of α-MSH was substituted with Ala at the mMC1R, mMC3R, and mMC4R, respectively. Importantly, the altered potencies due to Ala substitutions in α-MSH did not necessarily correlate with equivalent Ala substitutions in NDP-MSH, indicating that structural modifications and corresponding biological activities in one of these melanocortin ligands may not be predictive for the other agonist.

  20. Prototypical Rod Consolidation Demonstration Project

    International Nuclear Information System (INIS)

    1993-05-01

    The objective of Phase 3 of the Prototypical Rod consolidation Demonstration Project (PRCDP) was to procure, fabricate, assemble, and test the Prototypical Rod consolidation System as described in the NUS Phase 2 Final Design Report. This effort required providing the materials, components, and fabricated parts which makes up all of the system equipment. In addition, it included the assembly, installation, and setup of this equipment at the Cold Test Facility. During the Phase 3 effort the system was tested on a component, subsystem, and system level. This volume 1, discusses the PRCDP Phase 3 Test Program that was conducted by the HALLIBURTON NUS Environmental Corporation under contract AC07-86ID12651 with the United States Department of Energy. This document, Volume 1, Book 5 discusses the following topics: Lower Cutting System Test Results and Analysis Report; NFBC Loading System Test Results and Analysis Report; Robotic Bridge Transporter Test Results and Analysis Report; RM-10A Remotec Manipulator Test Results and Analysis Report; and Manipulator Transporter Test Results and Analysis Report

  1. Dynamic underground stripping demonstration project

    International Nuclear Information System (INIS)

    Newmark, R.L.

    1992-04-01

    LLNL is collaborating with the UC Berkeley College of Engineering to develop and demonstrate a system of thermal remediation techniques for rapid cleanup of localized underground spills. Called dynamic stripping to reflect the rapid and controllable nature of the process, it will combine steam injection, direct electrical heating, and tomographic geophysical imaging in a cleanup of the LLNL gasoline spill. In the first eight months of the project, a Clean Site engineering test was conducted to prove the field application of the techniques. Tests then began on the contaminated site in FY 1992. This report describes the work at the Clean Site, including design and performance criteria, test results, interpretations, and conclusions. We fielded 'a wide range of new designs and techniques, some successful and some not. In this document, we focus on results and performance, lessons learned, and design and operational changes recommended for work at the contaminated site. Each section focuses on a different aspect of the work and can be considered a self-contained contribution

  2. Engineered Geothermal System Demonstration Project

    Energy Technology Data Exchange (ETDEWEB)

    Petty, Susan

    2014-06-19

    In June 2009, AltaRock Energy began field work on a project supported by the U.S. Department of Energy entitled “Use of Multiple Stimulations to Improve Economics of Engineered Geothermal Systems in Shallow High Temperature Intrusives.” The goal of the project was to develop an Engineered Geothermal System (EGS) in the portion of The Geysers geothermal field operated by the Northern California Power Agency (NCPA). The project encountered several problems while deepening Well E-7 which culminated in the suspension of field activities in September 2009. Some of the problems encountered are particular to The Geysers area, while others might be encountered in any geothermal field, and they might be avoided in future operations.

  3. Sediment Dispersal on the Topset of a Tectonically Active Shelf-edge Delta: an Interplay Between Sediment Supply and Subsidence, as Demonstrated for the Selenga River, Lake Baikal, Russia

    Science.gov (United States)

    Nittrouer, J. A.; Dong, T. Y.

    2017-12-01

    The Selenga River delta (Lake Baikal, Russia), located adjacent to an active rift margin and filling the world's deepest lake, represents one of the few examples of a modern shelf-edge system. Research into sediment dispersal on the Selenga delta was undertaken with the aim of linking topset morphodynamics and sediment accumulation patterns with the production of stratigraphy. The subaerial delta is constructed of three active lobes that receive varying amounts of water and sediment, distributed among a nine-order bifurcating channel network. Data from multiple expeditions include bathymetric and water-discharge measurements, side-scan images of the bed and banks, sediment samples, and bankline composition (including sediment type and vegetation). This information is analyzed to evaluate spatial variability in: 1. channel geometry, 2. boundary shear stress, 3. bedform size, and 4. bed sediment composition. The delta possesses downstream sediment fining, whereby median channel bed size decreases by two orders of magnitude over thirty kilometers, from a predominantly gravel and sand mixture near the delta apex to silt and fine sand at the lake interface. The location of gravel termination among distributary channels coincides with a reduction in sediment-transport capacity, as assessed by measurements of boundary shear stress. Interestingly, backwater hydrodynamics, which operate as important influences on bed grain size for many deltas, is not a major influence on the Selenga system. Instead, a non-linear downstream decrease in boundary shear stress arises due to partitioning of water among the bifurcating channel network. As has been documented in previous studies, gravel and coarse sand are absent on the delta foreset and bottomset (i.e., the rift axis), despite a continuous sediment feed from upstream that should provide the supply necessary to increase bed slope and enhance transport capacity over the delta. To reconcile this discrepancy, a tectonic timescale is

  4. Magnetic Launch Assist Demonstration Test

    Science.gov (United States)

    2001-01-01

    This image shows a 1/9 subscale model vehicle clearing the Magnetic Launch Assist System, formerly referred to as the Magnetic Levitation (MagLev), test track during a demonstration test conducted at the Marshall Space Flight Center (MSFC). Engineers at MSFC have developed and tested Magnetic Launch Assist technologies. To launch spacecraft into orbit, a Magnetic Launch Assist System would use magnetic fields to levitate and accelerate a vehicle along a track at very high speeds. Similar to high-speed trains and roller coasters that use high-strength magnets to lift and propel a vehicle a couple of inches above a guideway, a launch-assist system would electromagnetically drive a space vehicle along the track. A full-scale, operational track would be about 1.5-miles long and capable of accelerating a vehicle to 600 mph in 9.5 seconds. This track is an advanced linear induction motor. Induction motors are common in fans, power drills, and sewing machines. Instead of spinning in a circular motion to turn a shaft or gears, a linear induction motor produces thrust in a straight line. Mounted on concrete pedestals, the track is 100-feet long, about 2-feet wide and about 1.5-feet high. The major advantages of launch assist for NASA launch vehicles is that it reduces the weight of the take-off, the landing gear, the wing size, and less propellant resulting in significant cost savings. The US Navy and the British MOD (Ministry of Defense) are planning to use magnetic launch assist for their next generation aircraft carriers as the aircraft launch system. The US Army is considering using this technology for launching target drones for anti-aircraft training.

  5. AT13148, a first-in-class multi-AGC kinase inhibitor, potently inhibits gastric cancer cells both in vitro and in vivo

    Energy Technology Data Exchange (ETDEWEB)

    Xi, Yu [Tongji Hospital, Tongji Medical School, Huazhong University of Science and Technology, Wuhan, Hubei 430030 (China); Department of General Surgery, First Affiliated Hospital, School of Medicine, Shihezi University, Shihezi, Xinjiang 832008 (China); Niu, Jianhua [Department of General Surgery, First Affiliated Hospital, School of Medicine, Shihezi University, Shihezi, Xinjiang 832008 (China); Shen, Yun [Department of Gastroenterology, First Affiliated Hospital, School of Medicine, Shihezi University, Shihezi, Xinjiang 832008 (China); Li, Dongmei [Department of Biochemistry and Molecular Biology, School of Medicine, Shihezi University, Shihezi, Xinjiang 832008 (China); Peng, Xinyu, E-mail: pppengxinyu@sina.com [Department of General Surgery, First Affiliated Hospital, School of Medicine, Shihezi University, Shihezi, Xinjiang 832008 (China); Wu, Xiangwei, E-mail: wuxiangweiys@126.com [Department of General Surgery, First Affiliated Hospital, School of Medicine, Shihezi University, Shihezi, Xinjiang 832008 (China)

    2016-09-09

    The AGC kinase family is important cell proliferation and survival. Dysregulation of this family contributes to gastric cancer progression. Here, we evaluated the potential activity of AT13148, a first-in-class multi-AGC kinase inhibitor, against gastric cancer cells. Our results showed that AT13148 exerted potent cytotoxic and anti-proliferative activities against a panel human gastric cancer cell lines (HGC-27, AGS, SNU-601, N87 and MKN-28), possibly via inducing cancer cell apoptotic death. Apoptosis inhibition by the Caspase blockers dramatically attenuated AT13148-caused cytotoxicity against gastric cancer cells. Intriguingly, same AT13148 treatment was not cytotoxic/pro-apoptotic to the non-cancerous human gastric epithelial GEC-1 cells. At the signaling level, AT13148 treatment in gastric cancer cells dramatically suppressed activation of multiple AGC kinases, including Akt (at p-Thr-308), p70S6 kinase (p70S6K), glycogen synthase kinase 3β (GSK-3β) and p90 ribosomal S6 kinase (RSK). Our in vivo studies demonstrated that daily oral gavage of AT13148 at well-tolerated doses significantly inhibited HGC27 xenograft tumor growth in nude mice. AGC activity was also dramatically decreased in AT13148-administrated HGC27 tumors. Therefore, targeting AGC kinases by AT13148 demonstrates superior anti-gastric cancer activity both in vitro and in vivo. The preclinical results of this study support the progression of this molecule into future evaluation as a valuable anti-gastric cancer candidate. - Highlights: • AT13148 is cytotoxic and anti-proliferative to human gastric cancer cells. • AT13148 induces gastric cancer cell apoptotic death, inhibited by Caspase inhibitors. • AT13148 inactivates multiple AGC kinases in human gastric cancer cells. • AT13148 oral administration suppresses HGC27 xenograft growth in nude mice. • AT13148 oral administration inhibits multiple AGC kinases in HGC27 xenograft tumors.

  6. Prototype Morphing Fan Nozzle Demonstrated

    Science.gov (United States)

    Lee, Ho-Jun; Song, Gang-Bing

    2004-01-01

    Ongoing research in NASA Glenn Research Center's Structural Mechanics and Dynamics Branch to develop smart materials technologies for aeropropulsion structural components has resulted in the design of the prototype morphing fan nozzle shown in the photograph. This prototype exploits the potential of smart materials to significantly improve the performance of existing aircraft engines by introducing new inherent capabilities for shape control, vibration damping, noise reduction, health monitoring, and flow manipulation. The novel design employs two different smart materials, a shape-memory alloy and magnetorheological fluids, to reduce the nozzle area by up to 30 percent. The prototype of the variable-area fan nozzle implements an overlapping spring leaf assembly to simplify the initial design and to provide ease of structural control. A single bundle of shape memory alloy wire actuators is used to reduce the nozzle geometry. The nozzle is subsequently held in the reduced-area configuration by using magnetorheological fluid brakes. This prototype uses the inherent advantages of shape memory alloys in providing large induced strains and of magnetorheological fluids in generating large resistive forces. In addition, the spring leaf design also functions as a return spring, once the magnetorheological fluid brakes are released, to help force the shape memory alloy wires to return to their original position. A computerized real-time control system uses the derivative-gain and proportional-gain algorithms to operate the system. This design represents a novel approach to the active control of high-bypass-ratio turbofan engines. Researchers have estimated that such engines will reduce thrust specific fuel consumption by 9 percent over that of fixed-geometry fan nozzles. This research was conducted under a cooperative agreement (NCC3-839) at the University of Akron.

  7. The Magnetic Sentences Industry Game: A Competitive In-Class Experience of Business-Level Strategy

    Science.gov (United States)

    Casile, Maureen; Wheeler, Jane V.

    2005-01-01

    The Magnetic Sentences Industry Game is a high-energy in-class exercise designed to help students gain hands-on experience with setting, implementing, evaluating, and revising business-level strategy. Students compete in teams to create and market sentences using Magnetic Poetry (a product of Magnetic Poetry, Inc.). Revenues earned are highly…

  8. The Effects and Predictor Value of In-Class Texting Behavior on Final Course Grades

    Science.gov (United States)

    McDonald, Sylvia E.

    2013-01-01

    Cell phones have become a norm within the collegiate environment but little research has examined their impact on academic attainment. The purpose of this study was to examine the effects that in-class texting behavior had on the final grade score in a freshmen level introductory social science course. Students in three different sections were…

  9. Maxillary first molar extraction in Class II malocclusion : Follow-up studies on treatment effects

    NARCIS (Netherlands)

    Livas, Christos

    2015-01-01

    This PhD research investigated treatment effects of extraction of one and two maxillary first molars in Class II subdivision and Class II/1 malocclusion cases respectively from a longer time perspective. Private practice records were scrutinized to evaluate aspects of a treatment technique combining

  10. Implementation Integrity of Practice-Based Coaching: Preliminary Results from the BEST in CLASS Efficacy Trial

    Science.gov (United States)

    Sutherland, Kevin S.; Conroy, Maureen A.; Vo, Abigail; Ladwig, Crystal

    2015-01-01

    The purpose of this article is to describe the practice-based coaching model used in BEST in CLASS, a Tier-2 classroom-based intervention comprised of evidence-based instructional practices designed to prevent and ameliorate the chronic problem behaviors of young children at risk for the development of emotional/behavioral disorders. Following a…

  11. Measuring Teacher Implementation of the "BEST in CLASS" Intervention Program and Corollary Child Outcomes

    Science.gov (United States)

    Conroy, Maureen A.; Sutherland, Kevin S.; Algina, James J.; Wilson, Reynolds E.; Martinez, Jose R.; Whalon, Kelly J.

    2015-01-01

    This study is part of a larger randomized efficacy trial examining the impact of Behavioral, Emotional, and Social Training: Competent Learners Achieving School Success ("BEST in CLASS"), a Tier 2 intervention that targets the prevention of emotional/behavioral disorders in young, high risk children. In this investigation, we examined…

  12. Calderon cokemaking process/demonstration project

    International Nuclear Information System (INIS)

    Albert Calderon

    1998-01-01

    This project deals with the demonstration of a coking process using proprietary technology of Calderon, with the following objectives geared to facilitate commercialization: (i) making coke of such quality as to be suitable for use in hard-driving, large blast furnaces; (ii) providing proof that such process is continuous and environmentally closed to prevent emissions; (iii) demonstrating that high-coking-pressure (non-traditional) coal blends which cannot be safely charged into conventional by-product coke ovens can be used in the Calderon process; and (iv) demonstrating that coke can be produced economically, at a level competitive with coke imports. The activities of the past quarter were focused on the following: Consolidation of the project team-players; Recruiting Koppers Industries as an additional stakeholder; Developing a closed system for the production of binder pitch from tar in the Calderon coking process as the incentive for Koppers to join the team; Gathering appropriate equipment for conducting a set of experiments at bench scale to simulate tar quality produced from the Calderon coking process for the production of binder pitch; and Further progress made in the design of the commercial coking reactor

  13. Nuclear Desalination Demonstration Project (NDDP) in India

    International Nuclear Information System (INIS)

    Tewari, P.K.; Misra, B.M.

    2001-01-01

    In order to gainfully employ the years of experience and expertise in various aspects of desalination activity, BARC (India) has undertaken installation of a hybrid nuclear desalination plant coupled to 170 MW(e) PHWR station at Kalpakkam, Chennai in the Southeast coast of India. The integrated system, called the Nuclear Desalination Demonstration Project (NDDP), will thus meet the dual needs of process water for nuclear power plant and drinking water for the neighbouring people. NDDP aims for demonstrating the safe and economic production of good quality water by nuclear desalination of seawater. It comprises a 4500 m 3 /d Multistage Flash (MSF) and a 1800 m 3 /d Reverse Osmosis (RO) plant. MSF section uses low pressure steam from Madras Atomic Power Station (MAPS), Kalpakkam. The objectives of the NDDP (Kalpakkam) are as follows: to establish the indigenous capability for the design, manufacture, installation and operation of nuclear desalination plants; to generate necessary design inputs and optimum process parameters for large scale nuclear desalination plant; to serve as a demonstration project to IAEA welcoming participation from interested member states. The hybrid plant is envisaged to have a number of advantages: a part of high purity desalted water produced from MSF plant will be used for the makeup demineralised water requirement (after necessary polishing) for the power station; blending of the product water from RO and MSF plants would provide requisite quality drinking water; the RO plant will continue to be operated to provide the water for drinking purposes during the shutdown of the power station

  14. THOR Bench-Scale Steam Reforming Demonstration

    Energy Technology Data Exchange (ETDEWEB)

    D. W. Marshall; N. R. Soelberg; K. M. Shaber

    2003-05-01

    The Idaho Nuclear Technology and Engineering Center (INTEC) was home to nuclear fuel reprocessing activities for decades at the Idaho National Engineering and Environmental Laboratory. As a result of the reprocessing activities, INTEC has accumulated approximately one million gallons of acidic, radioactive, sodium-bearing waste (SBW). The purpose of this demonstration was to investigate a reforming technology, offered by THORsm Treatment Technologies, LLC, for treatment of SBW into a "road ready" waste form that would meet the waste acceptance criteria for the Waste Isolation Pilot Plant (WIPP). A non-radioactive simulated SBW was used based on the known composition of waste tank WM-180 at INTEC. Rhenium was included as a non-radioactive surrogate for technetium. Data was collected to determine the nature and characteristics of the product, the operability of the technology, the composition of the off-gases, and the fate of key radionuclides (cesium and technetium) and volatile mercury compounds. The product contained a low fraction of elemental carbon residues in the cyclone and filter vessel catches. Mercury was quantitatively stripped from the product but cesium, rhenium (Tc surrogate), and the heavy metals were retained. Nitrates were not detected in the product and NOx destruction exceeded 98%. The demonstration was successful.

  15. TWR Bench-Scale Steam Reforming Demonstration

    Energy Technology Data Exchange (ETDEWEB)

    Marshall, D.W.; Soelberg, N.R.

    2003-05-21

    The Idaho Nuclear Technology and Engineering Center (INTEC) was home to nuclear fuel reprocessing activities for decades at the Idaho National Engineering and Environmental Laboratory. As a result of the reprocessing activities, INTEC has accumulated approximately one million gallons of acidic, radioactive, sodium-bearing waste (SBW). The purpose of this demonstration was to investigate a reforming technology, offered by ThermoChem Waste Remediation, LLC, (TWR) for treatment of SBW into a ''road ready'' waste form that would meet the waste acceptance criteria for the Waste Isolation Pilot Plant (WIPP). TWR is the licensee of Manufacturing Technology Conservation International (MTCI) steam-reforming technology in the field of radioactive waste treatment. A non-radioactive simulated SBW was used based on the known composition of waste tank WM-180 at INTEC. Rhenium was included as a non-radioactive surrogate for technetium. Data was collected to determine the nature and characteristics of the product, the operability of the technology, the composition of the off-gases, and the fate of key radionuclides (cesium and technetium) and volatile mercury compounds. The product contained a low fraction of elemental carbon residues in the cyclone and filter vessel catches. Mercury was quantitatively stripped from the product but cesium, rhenium (Tc surrogate), and the heavy metals were retained. Nitrate residues were about 400 ppm in the product and NOx destruction exceeded 86%. The demonstration was successful.

  16. TWR Bench-Scale Steam Reforming Demonstration

    Energy Technology Data Exchange (ETDEWEB)

    D. W. Marshall; N. R. Soelberg

    2003-05-01

    The Idaho Nuclear Technology and Engineering Center (INTEC) was home to nuclear fuel reprocessing activities for decades at the Idaho National Engineering and Environmental Laboratory. As a result of the reprocessing activities, INTEC has accumulated approximately one million gallons of acidic, radioactive, sodium-bearing waste (SBW). The purpose of this demonstration was to investigate a reforming technology, offered by ThermoChem Waste Remediation, LLC, (TWR) for treatment of SBW into a "road ready" waste form that would meet the waste acceptance criteria for the Waste Isolation Pilot Plant (WIPP). TWR is the licensee of Manufacturing Technology Conservation International (MTCI) steam-reforming technology in the field of radioactive waste treatment. A non-radioactive simulated SBW was used based on the known composition of waste tank WM-180 at INTEC. Rhenium was included as a non-radioactive surrogate for technetium. Data was collected to determine the nature and characteristics of the product, the operability of the technology, the composition of the off-gases, and the fate of key radionuclides (cesium and technetium) and volatile mercury compounds. The product contained a low fraction of elemental carbon residues in the cyclone and filter vessel catches. Mercury was quantitatively stripped from the product but cesium, rhenium (Tc surrogate), and the heavy metals were retained. Nitrate residues were about 400 ppm in the product and NOx destruction exceeded 86%. The demonstration was successful.

  17. THOR Bench-Scale Steam Reforming Demonstration

    Energy Technology Data Exchange (ETDEWEB)

    Marshall, D.W.; Soelberg, N.R.; Shaber, K.M.

    2003-05-21

    The Idaho Nuclear Technology and Engineering Center (INTEC) was home to nuclear fuel reprocessing activities for decades at the Idaho National Engineering and Environmental Laboratory. As a result of the reprocessing activities, INTEC has accumulated approximately one million gallons of acidic, radioactive, sodium-bearing waste (SBW). The purpose of this demonstration was to investigate a reforming technology, offered by THORsm Treatment Technologies, LLC, for treatment of SBW into a ''road ready'' waste form that would meet the waste acceptance criteria for the Waste Isolation Pilot Plant (WIPP). A non-radioactive simulated SBW was used based on the known composition of waste tank WM-180 at INTEC. Rhenium was included as a non-radioactive surrogate for technetium. Data was collected to determine the nature and characteristics of the product, the operability of the technology, the composition of the off-gases, and the fate of key radionuclides (cesium and technetium) and volatile mercury compounds. The product contained a low fraction of elemental carbon residues in the cyclone and filter vessel catches. Mercury was quantitatively stripped from the product but cesium, rhenium (Tc surrogate), and the heavy metals were retained. Nitrates were not detected in the product and NOx destruction exceeded 98%. The demonstration was successful.

  18. THOR Bench-Scale Steam Reforming Demonstration

    International Nuclear Information System (INIS)

    Marshall, D.W.; Soelberg, N.R.; Shaber, K.M.

    2003-01-01

    The Idaho Nuclear Technology and Engineering Center (INTEC) was home to nuclear fuel reprocessing activities for decades at the Idaho National Engineering and Environmental Laboratory. As a result of the reprocessing activities, INTEC has accumulated approximately one million gallons of acidic, radioactive, sodium-bearing waste (SBW). The purpose of this demonstration was to investigate a reforming technology, offered by THORsm Treatment Technologies, LLC, for treatment of SBW into a ''road ready'' waste form that would meet the waste acceptance criteria for the Waste Isolation Pilot Plant (WIPP). A non-radioactive simulated SBW was used based on the known composition of waste tank WM-180 at INTEC. Rhenium was included as a non-radioactive surrogate for technetium. Data was collected to determine the nature and characteristics of the product, the operability of the technology, the composition of the off-gases, and the fate of key radionuclides (cesium and technetium) and volatile mercury compounds. The product contained a low fraction of elemental carbon residues in the cyclone and filter vessel catches. Mercury was quantitatively stripped from the product but cesium, rhenium (Tc surrogate), and the heavy metals were retained. Nitrates were not detected in the product and NOx destruction exceeded 98%. The demonstration was successful

  19. The Kwajalein bioremediation demonstration: Final technical report

    Energy Technology Data Exchange (ETDEWEB)

    Walker, J.R. Jr.; Walker, A.B.

    1994-12-01

    The US Army Kwajalein Atoll (USAKA) Base, located in the Republic of the Marshall Islands (RMI) in the east-central Pacific Ocean, has significant petroleum hydrocarbon contamination resulting from years of military activities. Because of its remoteness, the lack of on-site sophisticated remediation or waste disposal facilities, the amenability of petroleum hydrocarbons to biodegradation, and the year-round temperature favorable for microbial activity, USAKA requested, through the Hazardous Waste Remedial Actions Program (HAZWRAP), that a project be conducted to evaluate the feasibility of using bioremediation for environmental restoration of contaminated sites within the atoll. The project was conducted in four distinct phases: (1) initial site characterization and on-site biotreatability studies, (2) selection of the demonstration area and collection of soil columns, (3) laboratory column biotreatability studies, and (4) an on-site bioremediation demonstration. The results of phases (1) and (3) have been detailed in previous reports. This report summarizes the results of phases (1) and (3) and presents phases (2) and (4) in detail.

  20. The Kwajalein bioremediation demonstration: Final technical report

    International Nuclear Information System (INIS)

    Walker, J.R. Jr.; Walker, A.B.

    1994-12-01

    The US Army Kwajalein Atoll (USAKA) Base, located in the Republic of the Marshall Islands (RMI) in the east-central Pacific Ocean, has significant petroleum hydrocarbon contamination resulting from years of military activities. Because of its remoteness, the lack of on-site sophisticated remediation or waste disposal facilities, the amenability of petroleum hydrocarbons to biodegradation, and the year-round temperature favorable for microbial activity, USAKA requested, through the Hazardous Waste Remedial Actions Program (HAZWRAP), that a project be conducted to evaluate the feasibility of using bioremediation for environmental restoration of contaminated sites within the atoll. The project was conducted in four distinct phases: (1) initial site characterization and on-site biotreatability studies, (2) selection of the demonstration area and collection of soil columns, (3) laboratory column biotreatability studies, and (4) an on-site bioremediation demonstration. The results of phases (1) and (3) have been detailed in previous reports. This report summarizes the results of phases (1) and (3) and presents phases (2) and (4) in detail

  1. Integration Process for the Habitat Demonstration Unit

    Science.gov (United States)

    Gill, Tracy; Merbitz, Jerad; Kennedy, Kriss; Tri, Terry; Howe, A. Scott

    2010-01-01

    The Habitat Demonstration Unit (HDU) is an experimental exploration habitat technology and architecture test platform designed for analog demonstration activities The HDU project has required a team to integrate a variety of contributions from NASA centers and outside collaborators and poses a challenge in integrating these disparate efforts into a cohesive architecture To complete the development of the HDU from conception in June 2009 to rollout for operations in July 2010, a cohesive integration strategy has been developed to integrate the various systems of HDU and the payloads, such as the Geology Lab, that those systems will support The utilization of interface design standards and uniquely tailored reviews have allowed for an accelerated design process Scheduled activities include early fit-checks and the utilization of a Habitat avionics test bed prior to equipment installation into HDU A coordinated effort to utilize modeling and simulation systems has aided in design and integration concept development Modeling tools have been effective in hardware systems layout, cable routing and length estimation, and human factors analysis Decision processes on the shell development including the assembly sequence and the transportation have been fleshed out early on HDU to maximize the efficiency of both integration and field operations Incremental test operations leading up to an integrated systems test allows for an orderly systems test program The HDU will begin its journey as an emulation of a Pressurized Excursion Module (PEM) for 2010 field testing and then may evolve to a Pressurized Core Module (PCM) for 2011 and later field tests, depending on agency architecture decisions The HDU deployment will vary slightly from current lunar architecture plans to include developmental hardware and software items and additional systems called opportunities for technology demonstration One of the HDU challenges has been designing to be prepared for the integration of

  2. Pilot demonstrations of arsenic removal technologies.

    Energy Technology Data Exchange (ETDEWEB)

    Siegal Malcolm D.

    2004-09-01

    The Arsenic Water Technology Partnership (AWTP) program is a multi-year program funded by a congressional appropriation through the Department of Energy to develop and test innovative technologies that have the potential to reduce the costs of arsenic removal from drinking water. The AWTP members include Sandia National Laboratories, the American Water Works Association (Awwa) Research Foundation and WERC (A Consortium for Environmental Education and Technology Development). The program is designed to move technologies from bench-scale tests to field demonstrations. The Awwa Research Foundation is managing bench-scale research programs; Sandia National Laboratories is conducting the pilot demonstration program and WERC will evaluate the economic feasibility of the technologies investigated and conduct technology transfer activities. The objective of the Sandia Arsenic Treatment Technology Demonstration project (SATTD) is the field demonstration testing of both commercial and innovative technologies. The scope for this work includes: (1) Identification of sites for pilot demonstrations; (2) Accelerated identification of candidate technologies through Vendor Forums, proof-of-principle laboratory and local pilot-scale studies, collaboration with the Awwa Research Foundation bench-scale research program and consultation with relevant advisory panels; and (3) Pilot testing multiple technologies at several sites throughout the country, gathering information on: (a) Performance, as measured by arsenic removal; (b) Costs, including capital and Operation and Maintenance (O&M) costs; (c) O&M requirements, including personnel requirements, and level of operator training; and (d) Waste residuals generation. The New Mexico Environment Department has identified over 90 public water systems that currently exceed the 10 {micro}g/L MCL for arsenic. The Sandia Arsenic Treatment Technology Demonstration project is currently operating pilots at three sites in New Mexico. The cities of

  3. Adolescents with Major Depression Demonstrate Increased Amygdala Activation

    Science.gov (United States)

    Yang, Tony T.; Simmons, Alan N.; Matthews, Scott C.; Tapert, Susan F.; Frank, Guido K.; Max, Jeffrey E.; Bischoff-Grethe, Amanda; Lansing, Amy E.; Brown, Gregory; Strigo, Irina A.; Wu, Jing; Paulus, Martin P.

    2010-01-01

    Objective: Functional neuroimaging studies have led to a significantly deeper understanding of the underlying neural correlates and the development of several mature models of depression in adults. In contrast, our current understanding of the underlying neural substrates of adolescent depression is very limited. Although numerous studies have…

  4. Environmental Quality Technology Demonstration, Evaluation and Transfer Activities

    Science.gov (United States)

    1996-12-02

    find undiscovered archaeological sites. Pilot project needs to be implemented to reveal subpixel demixing showing actual ground cover. POINT OF... rendering NC fines inert have been investigated in the past with only limited success. However, two previous U.S. Army Environmental Center (USAEC...DESCRIPTION To assess the feasibility of a novel approach to remediation, rendering explosives harmless without significant risk. The goal of this project

  5. 1. Extraction and Demonstration of Uterotonic Activity from the Root ...

    African Journals Online (AJOL)

    user

    indigenous knowledge imparts is traditional. 7 medicine knowledge . These therapeutic practices have been in existence for hundreds of years before the development of conventional scientific medicines e.g. morphine from Papever somniferum, quinine from Cinchona officinalis, strychnine from Srychnox nuxvomicaand ...

  6. 1. Extraction and Demonstration of Uterotonic Activity from the Root ...

    African Journals Online (AJOL)

    user

    step in the processing of the root for analysis of its. 10 11 bioactivity is recommended , . Furthermore, the oil from the root of this plant could possess properties that may be useful medicinally and/or industrially. Plant oils are widely used in medicine, cosmetology, aromatherapy, biofuel production, and other. 12, 13, 14, 15, ...

  7. Clinically Available Medicines Demonstrating Anti-Toxoplasma Activity

    Science.gov (United States)

    Neville, Andrew J.; Zach, Sydney J.; Wang, Xiaofang; Larson, Joshua J.; Judge, Abigail K.; Davis, Lisa A.; Vennerstrom, Jonathan L.

    2015-01-01

    Toxoplasma gondii is an apicomplexan parasite of humans and other mammals, including livestock and companion animals. While chemotherapeutic regimens, including pyrimethamine and sulfadiazine regimens, ameliorate acute or recrudescent disease such as toxoplasmic encephalitis or ocular toxoplasmosis, these drugs are often toxic to the host. Moreover, no approved options are available to treat infected women who are pregnant. Lastly, no drug regimen has shown the ability to eradicate the chronic stage of infection, which is characterized by chemoresistant intracellular cysts that persist for the life of the host. In an effort to promote additional chemotherapeutic options, we now evaluate clinically available drugs that have shown efficacy in disease models but which lack clinical case reports. Ideally, less-toxic treatments for the acute disease can be identified and developed, with an additional goal of cyst clearance from human and animal hosts. PMID:26392504

  8. Extraction and Demonstration of Uterotonic Activity from the Root of ...

    African Journals Online (AJOL)

    Background: The root of Steganotaenia araliacea is used for assisting labour in folk medicine. Recent reports indicate that the root could possess uterotonic substances. Objectives: The study aimed to evaluate three methods for the extraction of uterotonic principles from the root of S. araliacea growing in Zambia. Methods: ...

  9. Dedication for Safety-Related Fuses used in Class-1E Power System

    International Nuclear Information System (INIS)

    Hong, Younghee

    2014-01-01

    The safety-related fuses used in class-1E power system provide overcurrent protection for electrical system and isolate the class 1E circuit from a fault or overload condition. These days, the number of nuclear grade suppliers has been reduced. Accordingly, commercial grade, instead of safety-related, fuses are procured and used in the utilities through the dedication process. Therefore, this paper introduces the commercial grade fuse dedication process/engineering and how to assure the quality requirements with this process and engineering. The fuses used in class-1E power system are to protect overcurrent and to isolate fault. Therefore the fuse for acceptance in order to improve the quality and reliability for commercial grade fuses shall be dedicated. The fuse resistance value may be useful as an indicator of acceptance. The current carrying capacity test can change the fuse performance properties. Therefore these critical characteristics are needed for additional review and analysis with fuse manufactures

  10. "BiC in Sustainability?" : die Transparenz von Best in Class-Nachhaltigkeitsfonds in Deutschland

    OpenAIRE

    Schäfer, Henry; Bauer, Fabian; Bracht, Felix

    2015-01-01

    Der vorliegende Beitrag analysiert in Deutschland vertriebene Publikumsinvestmentfonds, die den Anspruch als Nachhaltigkeitsfonds erheben und nach dem Best in Class-Ansatz (BiC-Ansatz) in Kombination mit einem Negative-Screening (verstanden als Ausschlusskriterien) konstruiert sind. Ziel ist es zu erheben, auf welche Weise die den Fonds vom Anbieter zugrunde gelegten BiC- und Screening-Methoden Anlegern transparent gemacht werden und worin zwischen den Fonds diesbezüglich Unterschiede bestehe...

  11. Oak Ridge greater confinement disposal demonstrations

    International Nuclear Information System (INIS)

    Van Hoesen, S.D.; Clapp, R.B.

    1987-01-01

    Demonstrations are being conducted in association with the disposal of a high activity low-level waste (LLW) stream. The waste stream in question will result from the cement solidification of decanted liquids from the Melton Valley Storage Tanks (MVST). The solid waste will be produced beginning in mid summer 1988. It is anticipated to have significant concentrations of Cs-137 and Sr-90, with smaller amounts of other radionuclides and <100 nCi/gm of TRU. The solid waste forms are expected to have surface dose rates in the 1 to 2 r/hr range. The solid waste will also contain several chemical species at concentrations which are below those of concern, but which may present enhanced corrosion potential for the disposal units. 2 refs., 5 figs

  12. Concentrating Solar Power Gen3 Demonstration Roadmap

    Energy Technology Data Exchange (ETDEWEB)

    Mehos, Mark [National Renewable Energy Lab. (NREL), Golden, CO (United States); Turchi, Craig [National Renewable Energy Lab. (NREL), Golden, CO (United States); Vidal, Judith [National Renewable Energy Lab. (NREL), Golden, CO (United States); Wagner, Michael [National Renewable Energy Lab. (NREL), Golden, CO (United States); Ma, Zhiwen [National Renewable Energy Lab. (NREL), Golden, CO (United States); Ho, Clifford [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); Kolb, William [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); Andraka, Charles [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); Kruizenga, Alan [Sandia National Lab. (SNL-CA), Livermore, CA (United States)

    2017-01-01

    Today's power-tower concentrating solar power (CSP) technology exists in large part as a result of Department of Energy (DOE) and utility industry funding of demonstration systems in the 1980s and 1990s. Today's most advanced towers are integrated with molten-salt thermal energy storage, delivering thermal energy at 565 degrees C for integration with conventional steam-Rankine cycles. The supercritical carbon dioxide power cycle has been identified as a likely successor to the steam-Rankine power cycle due to its potential for high efficiency when operating at elevated temperatures of 700 degrees C or greater. Over the course of the SunShot Initiative, DOE has supported a number of technology pathways that can operate efficiently at these temperatures and that hold promise to be reliable and cost effective. Three pathways - molten salt, particle, and gaseous - were selected for further investigation based on a two-day workshop held in August of 2016. The information contained in this roadmap identifies research and development challenges and lays out recommended research activities for each of the three pathways. DOE foresees that by successfully addressing the challenges identified in this roadmap, one or more technology pathways will be positioned for demonstration and subsequent commercialization within the next ten years. Based on current knowledge of the three power tower technologies, all three have the potential to achieve the SunShot goal of 6 cents/kilowatt-hour. Further development, modeling, and testing are now required to bring one or more of the technologies to a stage where integrated system tests and pilot demonstrations are feasible.

  13. Comparative study of palatal rugae pattern in class II div 1 and class I individuals

    Directory of Open Access Journals (Sweden)

    Chandrasekhar Gandikota

    2012-01-01

    Full Text Available Aims: To determine if the palatal rugae have a characteristic pattern in untreated class II div 1 malocclusions compared to normal class I occlusions, and to provide a valuable insight whether palatal rugae can be taken up as additional criteria for classifying malocclusions. Materials and Methods: The study was conducted on initial maxillary dental casts of 24 individuals with untreated class II div 1 malocclusion with an overjet of minimum of 5mm, of whom 12 were females and 12 were males, with age ranging from 16 to 24 years and compared with Class I patients casts. Results: There was no statistically significant difference in the mean intermolar widths of the two groups. The first, second and third palatal rugae were shorter in class II div 1 patients than in class I patients which was statistically significant. All the patients with Angle′s class I occlusion had good pattern of palatal rugae falling in the score range of 1-3, whereas 22% of the patients with class II div 1 had poor pattern of palatal rugae, with score 4 and 2 patients exhibiting a score of 4 and 5, respectively, but this was not statistically significant. Conclusion: There was a significant constriction of the palatal rugae in class II div 1 individuals as compared to class I individuals, though they were matched for the same intermolar widths. There was a distinct pattern of palatal rugae between the two groups.

  14. Comparative study of palatal rugae pattern in class II div 1 and class I individuals.

    Science.gov (United States)

    Gandikota, Chandrasekhar; Venkata, Yudhister Palla; Challa, Padmalatha; Juvvadi, Shubhaker Rao; Mathur, Anirudh

    2012-08-01

    To determine if the palatal rugae have a characteristic pattern in untreated class II div 1 malocclusions compared to normal class I occlusions, and to provide a valuable insight whether palatal rugae can be taken up as additional criteria for classifying malocclusions. The study was conducted on initial maxillary dental casts of 24 individuals with untreated class II div 1 malocclusion with an overjet of minimum of 5mm, of whom 12 were females and 12 were males, with age ranging from 16 to 24 years and compared with Class I patients casts. There was no statistically significant difference in the mean intermolar widths of the two groups. The first, second and third palatal rugae were shorter in class II div 1 patients than in class I patients which was statistically significant. All the patients with Angle's class I occlusion had good pattern of palatal rugae falling in the score range of 1-3, whereas 22% of the patients with class II div 1 had poor pattern of palatal rugae, with score 4 and 2 patients exhibiting a score of 4 and 5, respectively, but this was not statistically significant. There was a significant constriction of the palatal rugae in class II div 1 individuals as compared to class I individuals, though they were matched for the same intermolar widths. There was a distinct pattern of palatal rugae between the two groups.

  15. Exploration Medical System Demonstration (EMSD) Project

    Science.gov (United States)

    Chin, Duane

    2012-01-01

    The Exploration Medical System Demonstration (EMSD) is a project under the Exploration Medical Capability (ExMC) element managed by the Human Research Program (HRP). The vision for the EMSD is to utilize ISS as a test bed to show that several medical technologies needed for an exploration mission and medical informatics tools for managing evidence and decision making can be integrated into a single system and used by the on-orbit crew in an efficient and meaningful manner. Objectives: a) Reduce and even possibly eliminate the time required for on-orbit crew and ground personnel (which include Surgeon, Biomedical Engineer (BME) Flight Controller, and Medical Operations Data Specialist) to access and move medical data from one application to another. b) Demonstrate that the on-orbit crew has the ability to access medical data/information using an intuitive and crew-friendly software solution to assist/aid in the treatment of a medical condition. c) Develop a common data management framework and architecture that can be ubiquitously used to automate repetitive data collection, management, and communications tasks for all crew health and life sciences activities.

  16. Peers and Academic Achievement: A Longitudinal Study on Selection and Socialization Effects of In-Class Friends

    Science.gov (United States)

    Fortuin, Janna; van Geel, Mitch; Vedder, Paul

    2016-01-01

    The present study was conducted to analyze whether in-class friends influence each other's grades, and whether adolescents tend to select friends that are similar to them in terms of academic achievement. During 1 academic year, 542 eighth-grade students (M age = 13.3 years) reported on 3 different occasions on their in-class friendship networks.…

  17. Atlanta congestion reduction demonstration. National evaluation : content analysis test plan.

    Science.gov (United States)

    2011-08-01

    This report presents the test plan for collecting and analyzing information on outreach activities, media : coverage, and establishment of the partnership for the projects comprising the Atlanta Congestion : Reduction Demonstration (CRD) under the Un...

  18. Classroom as Reality: Demonstrating Campaign Effects through Live Simulation

    Science.gov (United States)

    Coffey, Daniel J.; Miller, William J.; Feuerstein, Derek

    2011-01-01

    Scholastic research has demonstrated that when conducted properly, active learning exercises are successful at increasing student awareness, student interest, and knowledge retention. Face-to-face simulations, in particular, have been demonstrated to add positively to classrooms focusing on comparative politics, international relations, public…

  19. 78 FR 22527 - TRICARE Access to Care Demonstration Project

    Science.gov (United States)

    2013-04-16

    ... DEPARTMENT OF DEFENSE Office of the Secretary TRICARE Access to Care Demonstration Project AGENCY... is to advise interested parties of a 2-year extension of the demonstration project in which the... project will continue through May 4, 2015. ADDRESSES: TRICARE Management Activity, Health Plan Operations...

  20. Calderon cokemaking process/demonstration project

    International Nuclear Information System (INIS)

    Albert Calderon

    1996-01-01

    This project which deals with the demonstration of a full size commercial coking retort using Calderon's proprietary technology for making metallurgical coke ran into a commercialization problem by virtue that the designed retort for two (2) tons of coke/hour necessitates thirty-two (32) retorts to produce the 500,000 tons of coke per year for a commercial plant. Bechtel Mining and Metals prepared a cost estimate of the commercial plant which indicated the commercial plant would not be economically feasible. The activity during this reporting period was directed to making changes to the design of the coking retort in order to reduce the number of retorts required for a 500,000 ton/year commercial coke facility. The result of this activity resulted in the drastic reduction of the number of retorts to eight (8) with each retort projected to produce 8.17 tons of coke/hour. Such decrease in number of retorts makes the Calderon technology quite competitive and therefore commercially feasible

  1. UTM Data Working Group Demonstration 1: Final Report

    Science.gov (United States)

    Rios, Joseph L.; Mulfinger, Daniel G.; Smith, Irene S.; Venkatesan, Priya; Smith, David R.; Baskaran, Vijayakumar; Wang, Leo

    2017-01-01

    This document summarizes activities defining and executing the first demonstration of the NASA-FAA Research Transition Team (RTT) Data Exchange and Information Architecture (DEIA) working group (DWG). The demonstration focused on testing the interactions between two key components in the future UAS Traffic Management (UTM) System through a collaborative and distributed simulation of key scenarios. The summary incorporates written feedback from each of the participants in the demonstration. In addition to reporting the activities, this report also provides some insight into future steps of this working group.

  2. The contribution of different frequency bands in class separability of covert speech tasks for BCIs.

    Science.gov (United States)

    Jahangiri, Amir; Sepulveda, Francisco

    2017-07-01

    Several recent studies demonstrate the possibility of using user initiated covert speech mental tasks in brain computer interfaces with varying degrees of success, but details of the best frequency features had not been investigated. In this work, ten volunteers in the age range of 22-70 years participated in the experiment. Eight of them were neurologically healthy, one user was dyslexic, and another was autistic. The four words "back", "forward", "left", and "right" were shortened into "BA", "FO", "LE", and "RY", which are phonetically dissimilar and cognitively relevant directional commands. Participants were asked to covertly speak each as soon as the letters appeared on a screen. Volunteers completed five recording runs. During each run the four words were presented in random succession to avoid sequence bias. The recorded EEG data from the ten users were analysed to discover the best features within a Gabor Transform of the signals, i.e., those yielding the highest word-pair classification accuracy for this specific type of linguistic mental activity. Using this BCI, suitable class separability of covert speech tasks is confirmed for all, including disabled users, with consistently high classification accuracy from 72% to 88% in all cases. Like motor imagery tasks, Alpha and Beta band activity were found to contain 12% and 31% of the most important features respectively. Gamma band activity, which indicates high mental functions, contains 57% of the most important features in this study.

  3. Proposed demonstration projects matrix, commercial buildings

    Energy Technology Data Exchange (ETDEWEB)

    None

    1976-04-01

    The Proposed Demonstration Projects Matrix is designed to meet the goals and objectives of ERDA-23A. Selection criteria for the Demonstration Matrix have been established. Phase 0 SHACOB studies have been updated and expanded. Building/location/solar system combinations have been selected for the directed level of 200 commercial demonstrations. The list of demonstrations has been arranged in an effectiveness ranked array. Modeling techniques have been applied to the selection process to enable mechanized generation of similar listings at other demonstration levels. Individual demonstration project selection guidelines have been developed to provide decision criteria among candidate projects. (WDM)

  4. Customer-focused planning: Demonstration project summaries

    Energy Technology Data Exchange (ETDEWEB)

    George, S.S. (Putnam, Hayes and Bartlett, Inc., San Francisco, CA (United States))

    1992-12-01

    To succeed in the increasingly competitive and dynamic markets in which they operate, electric utilities are focusing ever greater attention on understanding and meeting customer needs. EPRI's Customer Focused Planning (CFP) project was established to develop concepts and tools that will help utilities enhance their commitment to customer service. The project team conducted a series of interviews and meetings with participating utilities to collaboratively implement crucial steps in the CFP process. Although there is no unique set of tools or single management approach for improving product and service delivery, customer-focused companies have at least five ideals in common. They (1) define goals and objectives in concrete terms, (2) extend the planning boundaries of the organization to include all members of the energy services infrastructure, (3) painstakingly link functional activities directly to customer needs, (4) incorporate the customer's voice in new product/ service design, and (5) align performance measures with customer needs. In addition, customer-focused companies use a variety of methods to improve customer satisfaction and company performance. These methods include conducting market research, developing market processes such as demand-side management contracting or bidding to reveal customer preferences, and involving customers more directly in the planning process. This report summarizes two brief demonstration projects conducted as part of EPRI's CFP project, one at Commonwealth Edison Company (CECo) and one at PSI Energy. The CECo project emphasized developing customer-focused performance measures for telephone inquiries. The PSI Energy project involved a one-day workshop underscoring two important CFP elements-understanding customer wants and explicitly linking those wants to utility activities.

  5. HTI retrieval demonstration project execution plan

    International Nuclear Information System (INIS)

    Ellingson, D.R.

    1997-01-01

    This plan describes the process for demonstrating the retrieval of difficult Hanford tank waste forms utilizing commercial technologies and the private sector to conduct the operations. The demonstration is to be conducted in Tank 241-C-106

  6. Preliminary materials assessment in solar demonstration systems

    Energy Technology Data Exchange (ETDEWEB)

    Cheng, C. F.

    1978-11-01

    A preliminary assessment of materials performance in solar demonstration system are reviewed from published literature and limited service experience. The review is a summary of the solar demonstration systems and the materials used in the collector and transport systems.

  7. Learning Demonstration Progress Report -- September 2007

    Energy Technology Data Exchange (ETDEWEB)

    Wipke, K.; Sprik, S.; Kurtz, J.; Thomas, H.

    2007-11-01

    This report documents the key results from the DOE Controlled Hydrogen Fleet and Infrastructure Validation and Demonstration project. This project is also referred to as the fuel cell vehicle and infrastructure learning demonstration.

  8. An Underground Storage Tank Integrated Demonstration report

    International Nuclear Information System (INIS)

    Quadrel, M.J.; Hunter, V.L.; Young, J.K.; Lini, D.C.; Goldberg, C.

    1993-04-01

    The Waste Characterization Data and Technology Development Needs Assessment provides direct support to the Underground Storage Tank Integrated Demonstration (UST-ID). Key users of the study's products may also include individuals and programs within the US Department of Energy (DOE) Office of Technology Development (EM-50), the Office of Waste Operations (EM-30), and the Office of Environmental Restoration (EM-40). The goal of this work is to provide the UST-ID with a procedure for allocating funds across competing characterization technologies in a timely and defensible manner. It resulted in three primary products: 1. It organizes and summarizes information on underground storage tank characterization data needs. 2. It describes current technology development activity related to each need and flags areas where technology development may be beneficial. 3. It presents a decision process, with supporting software, for evaluating, prioritizing, and integrating possible technology development funding packages. The data presented in this document can be readily updated as the needs of the Waste Operations and Environmental Restoration programs mature and as new and promising technology development options emerge

  9. Demonstration tokamak-power-plant study (DEMO)

    International Nuclear Information System (INIS)

    1982-09-01

    A study of a Demonstration Tokamak Power Plant (DEMO) has been completed. The study's objective was to develop a conceptual design of a prototype reactor which would precede commercial units. Emphasis has been placed on defining and analyzing key design issues and R and D needs in five areas: noninductive current drivers, impurity control systems, tritium breeding blankets, radiation shielding, and reactor configuration and maintenance features. The noninductive current drive analysis surveyed a wide range of candidates and selected relativistic electron beams for the reference reactor. The impurity control analysis considered both a single-null poloidal divertor and a pumped limiter. A pumped limiter located at the outer midplane was selected for the reference design because of greater engineering simplicity. The blanket design activity focused on two concepts: a Li 2 O solid breeder with high pressure water cooling and a lead-rich Li-Pb eutectic liquid metal breeder (17Li-83Pb). The reference blanket concept is the Li 2 O option with a PCA structural material. The first wall concept is a beryllium-clad corrugated panel design. The radiation shielding effort concentrated on reducing the cost of bulk and penetration shielding; the relatively low-cost outborad shield is composed of concrete, B 4 C, lead, and FE 1422 structural material

  10. MODIL cryocooler producibility demonstration project results

    Energy Technology Data Exchange (ETDEWEB)

    Cruz, G.E.; Franks, R.M.

    1993-04-07

    The production of large quantities of spacecraft needed by SDIO will require a cultural change in design and production practices. Low rates production and the need for exceedingly high reliability has driven the industry to custom designed, hand crafted, and exhaustively tested satellites. These factors have mitigated against employing design and manufacturing cost reduction methods commonly used in tactical missile production. Additional challenges to achieving production efficiencies are presented by the SDI spacecraft mission requirement. IR sensor systems, for example, are comprised of subassemblies and components that require the design, manufacture, and maintenance of ultra precision tolerances over challenging operational lifetimes. These IR sensors demand the use of reliable, closed loop, cryogenic refrigerators or active cryocoolers to meet stringent system acquisition and pointing requirements. The authors summarize some spacecraft cryocooler requirements and discuss observations regarding Industry`s current production capabilities of cryocoolers. The results of the Lawrence Livermore National Laboratory (LLNL) Spacecraft Fabrication and Test (SF and T) MODIL`s Phase I producibility demonstration project is presented.

  11. Chilaiditi's syndrome demonstrated by SPECT/CT

    Directory of Open Access Journals (Sweden)

    Nalini S Perumal

    2009-11-01

    Full Text Available Purpose: Chilaiditi’s syndrome is a rare condition commonly diagnosed as an incidental radiological finding. The aim of this report is to show the role of SPECT-CT in this syndrome and state the functional and anatomical role of this hybrid imaging modality. Materials and Methods: A case report. Results: A 49-year-old female patient was referred for gallium-67 citrate for a possible granulomatous myositis and underwent SPECT-CT of the abdomen to assess the area of decreased gallium uptake on planar images of the liver. The combined SPECT and CT modality demonstrated findings consistent with the clinical evidence of Chilaiditi’s syndrome. The anatomical part of this hybrid modality made it easier to evaluate the area of gallium lack of uptake which was due to air in the colon. Conclusion: This case does not only show the role of SPECT-CT in this syndrome but also suggest that the use of such modality should be considered whenever available in the evaluation of patients in whom the localization of active disease becomes imperative.

  12. Advanced Communications Architecture Demonstration Made Significant Progress

    Science.gov (United States)

    Carek, David Andrew

    2004-01-01

    Simulation for a ground station located at 44.5 deg latitude. The Advanced Communications Architecture Demonstration (ACAD) is a concept architecture to provide high-rate Ka-band (27-GHz) direct-to-ground delivery of payload data from the International Space Station. This new concept in delivering data from the space station targets scientific experiments that buffer data onboard. The concept design provides a method to augment the current downlink capability through the Tracking Data Relay Satellite System (TDRSS) Ku-band (15-GHz) communications system. The ACAD concept pushes the limits of technology in high-rate data communications for space-qualified systems. Research activities are ongoing in examining the various aspects of high-rate communications systems including: (1) link budget parametric analyses, (2) antenna configuration trade studies, (3) orbital simulations (see the preceding figure), (4) optimization of ground station contact time (see the following graph), (5) processor and storage architecture definition, and (6) protocol evaluations and dependencies.

  13. MODIL cryocooler producibility demonstration project results

    Energy Technology Data Exchange (ETDEWEB)

    Cruz, G.E.; Franks, R.M.

    1993-06-24

    The production of large quantities of spacecraft needed by SDIO will require a cultural change in design and production practices. Low rates production and the need for exceedingly high reliability has driven the industry to custom designed, hand crafted, and exhaustingly tested satellites. These factors have mitigated against employing design and manufacturing cost reduction methods commonly used in tactical missile production. Additional challenges to achieving production efficiencies are presented by the SDI spacecraft mission requirement. IR sensor systems, for example, are comprised of subassemblies and components that require the design, manufacture, and maintenance of ultra precision tolerances over challenging operational lifetimes. These IR sensors demand the use of reliable, closed loop, cryogenic refrigerators or active cryocoolers to meet stringent system acquisition and pointing requirements. The authors summarize some spacecraft cryocooler requirements and discuss their observations regarding Industry`s current production capabilities of cryocoolers. The results of the Lawrence Livermore National Laboratory (LLNL) Spacecraft Fabrication and Test (SF and T) MODIL`s Phase I producibility demonstration project are presented. The current project that involves LLNL and industrial participants is discussed.

  14. 40 CFR 117.14 - Demonstration projects.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 21 2010-07-01 2010-07-01 false Demonstration projects. 117.14 Section... DETERMINATION OF REPORTABLE QUANTITIES FOR HAZARDOUS SUBSTANCES Applicability § 117.14 Demonstration projects... research or demonstration projects relating to the prevention, control, or abatement of hazardous substance...

  15. Controlled Hydrogen Fleet and Infrastructure Demonstration Project

    Energy Technology Data Exchange (ETDEWEB)

    Dr. Scott Staley

    2010-03-31

    This program was undertaken in response to the US Department of Energy Solicitation DE-PS30-03GO93010, resulting in this Cooperative Agreement with the Ford Motor Company and BP to demonstrate and evaluate hydrogen fuel cell vehicles and required fueling infrastructure. Ford initially placed 18 hydrogen fuel cell vehicles (FCV) in three geographic regions of the US (Sacramento, CA; Orlando, FL; and southeast Michigan). Subsequently, 8 advanced technology vehicles were developed and evaluated by the Ford engineering team in Michigan. BP is Ford's principal partner and co-applicant on this project and provided the hydrogen infrastructure to support the fuel cell vehicles. BP ultimately provided three new fueling stations. The Ford-BP program consists of two overlapping phases. The deliverables of this project, combined with those of other industry consortia, are to be used to provide critical input to hydrogen economy commercialization decisions by 2015. The program's goal is to support industry efforts of the US President's Hydrogen Fuel Initiative in developing a path to a hydrogen economy. This program was designed to seek complete systems solutions to address hydrogen infrastructure and vehicle development, and possible synergies between hydrogen fuel electricity generation and transportation applications. This project, in support of that national goal, was designed to gain real world experience with Hydrogen powered Fuel Cell Vehicles (H2FCV) 'on the road' used in everyday activities, and further, to begin the development of the required supporting H2 infrastructure. Implementation of a new hydrogen vehicle technology is, as expected, complex because of the need for parallel introduction of a viable, available fuel delivery system and sufficient numbers of vehicles to buy fuel to justify expansion of the fueling infrastructure. Viability of the fuel structure means widespread, affordable hydrogen which can return a reasonable profit to

  16. Craniofacial changes in Class III malocclusion as related to skeletal and dental maturation.

    Science.gov (United States)

    Baccetti, Tiziano; Reyes, Brian C; McNamara, James A

    2007-08-01

    In this large cross-sectional study, we aimed to analyze growth trends in white subjects with Class III malocclusion using both skeletal and dental maturation staging. The sample consisted of 1091 pretreatment lateral cephalometric records of Class III patients (560 female, 531 male). Cephalometric dentoskeletal measurements were compared at subsequent stages in cervical vertebral maturation and Hellman's categorization of dental development by means of ANOVA with Bonferroni post-hoc tests in both sexes separately. The findings indicated that, in Class III malocclusion, the pubertal peak in mandibular growth occurs between stages 3 and 4 of cervical vertebral maturation, with average increases in total mandibular length of about 8 and 5.5 mm in Class III boys and girls, respectively. Significant changes in total mandibular length occur until young adulthood (18 years on average), with increases between late maturation stages (4 through 6) that were twice as large as in subjects with normal occlusion for the Class III females, and 3 times as large as in subjects with normal occlusion for the Class III males. Growth trends toward accentuated Class III profile and increased vertical dimension of the face also become apparent at late developmental stages (corresponding with complete eruption of the second and third molars).

  17. Soft tissue profile changes after Functional Mandibular Advancer or Herbst appliance treatment in class II patients.

    Science.gov (United States)

    Hourfar, Jan; Lisson, Jörg Alexander; Gross, Ulrich; Frye, Linda; Kinzinger, Gero Stefan Michael

    2018-03-01

    The objective of the present study is to compare the effects on soft tissue profile in class II patients after treatment with either "Functional Mandibular Advancer" (FMA) or Herbst appliance. The study included n = 42 patients treated with either FMA (n = 21) or Herbst appliance (n = 21) by the same experienced orthodontist. The treatment followed a single-step advancement protocol. Lateral cephalograms were analyzed through a set of customized measurements. The actual therapeutic effect was calculated using data from a growth survey. After testing for normal distribution and homogeneity of variance, data were analyzed by one-sample Student's t tests and independent Student's t tests. Statistical significance was set at p profile were found in FMA and Herbst appliance patients. All remaining variables revealed no significant differences. Treatment-related changes on the facial soft tissue profile could be regarded similar in class II patients treated with FMA or Herbst appliance. No treatment-related changes that were specific for FMA or Herbst appliance could be identified. Only moderate changes were noted comparing pre- and posttreatment soft tissue profiles. Despite proven differences in skeletal and dental treatment effects, the facial profile has not to be taken into consideration when choosing between FMA and Herbst appliance for class II treatment.

  18. Changes in skeletal and dental relationship in Class II Division I malocclusion after rapid maxillary expansion: a prospective study.

    Science.gov (United States)

    Baratieri, Carolina; Alves, Matheus; Bolognese, Ana Maria; Nojima, Matilde C G; Nojima, Lincoln I

    2014-01-01

    To assess skeletal and dental changes immediately after rapid maxillary expansion (RME) in Class II Division 1 malocclusion patients and after a retention period, using cone beam computed tomography (CBCT) imaging. Seventeen children with Class II, Division 1 malocclusion and maxillary skeletal transverse deficiency underwent RME following the Haas protocol. CBCT were taken before treatment (T1), at the end of the active expansion phase (T2) and after a retention period of 6 months (T3). The scanned images were measured anteroposteriorly (SNA, SNB, ANB, overjet and MR) and vertically (N-ANS, ANS-Me, N-Me and overbite). Significant differences were identified immediately after RME as the maxilla moved forward, the mandible moved downward, overjet increased and overbite decreased. During the retention period, the maxilla relapsed backwards and the mandible was displaced forward, leaving patients with an overall increase in anterior facial height. RME treatment allowed more anterior than inferior positioning of the mandible during the retention period, thus significantly improving Class II dental relationship in 75% of the patients evaluated.

  19. Virtual Exploitation Environment Demonstration for Atmospheric Missions

    Science.gov (United States)

    Natali, Stefano; Mantovani, Simone; Hirtl, Marcus; Santillan, Daniel; Triebnig, Gerhard; Fehr, Thorsten; Lopes, Cristiano

    2017-04-01

    The scientific and industrial communities are being confronted with a strong increase of Earth Observation (EO) satellite missions and related data. This is in particular the case for the Atmospheric Sciences communities, with the upcoming Copernicus Sentinel-5 Precursor, Sentinel-4, -5 and -3, and ESA's Earth Explorers scientific satellites ADM-Aeolus and EarthCARE. The challenge is not only to manage the large volume of data generated by each mission / sensor, but to process and analyze the data streams. Creating synergies among the different datasets will be key to exploit the full potential of the available information. As a preparation activity supporting scientific data exploitation for Earth Explorer and Sentinel atmospheric missions, ESA funded the "Technology and Atmospheric Mission Platform" (TAMP) [1] [2] project; a scientific and technological forum (STF) has been set-up involving relevant European entities from different scientific and operational fields to define the platforḿs requirements. Data access, visualization, processing and download services have been developed to satisfy useŕs needs; use cases defined with the STF, such as study of the SO2 emissions for the Holuhraun eruption (2014) by means of two numerical models, two satellite platforms and ground measurements, global Aerosol analyses from long time series of satellite data, and local Aerosol analysis using satellite and LIDAR, have been implemented to ensure acceptance of TAMP by the atmospheric sciences community. The platform pursues the "virtual workspace" concept: all resources (data, processing, visualization, collaboration tools) are provided as "remote services", accessible through a standard web browser, to avoid the download of big data volumes and for allowing utilization of provided infrastructure for computation, analysis and sharing of results. Data access and processing are achieved through standardized protocols (WCS, WPS). As evolution toward a pre

  20. [Quantified research about the effects of sleep quality on attention in class and acadamic achievements in primary school children].

    Science.gov (United States)

    Wang, Xiang-Yun; Qian, Yan-Fei; Gong, Sheng-Cheng; Tan, Mo; Tan, Xin; Yang, Yan; Li, Ling-Di; Huang, Chao-Quan

    2011-12-01

    To study the adverse effects of sleep problems and sleep insufficiency on attention in class and pupils' acadamic achievements. A total of 1138 students from four primary schools at ages of 6-12 years were randomly sampled from four districts of Changsha city, Hunan Province June 2009 to April 2010. The inquired items included sleep problems, sleep time, sleep quality, attention in class and academic achievements. Teachers and parents observed the pupils according to the unified requirements for 3 months and then filled out the questionnaires. The total valid inquiry tables were 1091 with the answering rate of 95.87%, including 549 boys and 542 girls. The sleep quality was more poor in children with sleep problems or sleep insufficiency than in children with normal sleep. The sleep quality was reduced and aggravated along with the increasing sleep problems and the reducing sleep time. The attention in class and academic achievements in children with sleep problems or sleep insufficiency were more poor than in children with normal sleep. The sleep quality index was negatively correlated with attention in class and academic achievements. The attention in class was positively correlated with academic achievements. The decline in sleep quality directly affects the attention in class and reduce the academic achievements in primary school children.

  1. A Blended Learning Approach to Teaching Project Management: A Model for Active Participation and Involvement: Insights from Norway

    Directory of Open Access Journals (Sweden)

    Bassam A. Hussein

    2015-04-01

    Full Text Available The paper demonstrates and evaluates the effectiveness of a blended learning approach to create a meaningful learning environment. We use the term blended learning approach in this paper to refer to the use of multiple or hybrid instructional methods that emphasize the role of learners as contributors to the learning process rather than recipients of learning. Contribution to learning is attained by using in class gaming as pathways that ensure active involvement of learners. Using a blended learning approach is important in order to be able to address different learning styles of the target group. The approach was also important in order to be able to demonstrate different types of challenges, issues and competences needed in project management. Student evaluations of the course confirmed that the use of multiple learning methods and, in particular, in class gaming was beneficial and contributed to a meaningful learning experience.

  2. Demonstration of Resolving Urban Problems by Applying Smart Technology.

    Science.gov (United States)

    Kim, Y.

    2016-12-01

    Recently, movements to seek various alternatives are becoming more active around the world to resolve urban problems related to energy, water, a greenhouse gas, and disaster by utilizing smart technology system. The purpose of this study is to evaluate service verification aimed at demonstration region applied with actual smart technology in order to raise the efficiency of the service and explore solutions for urban problems. This process must be required for resolving urban problems in the future and establishing `integration platform' for sustainable development. The demonstration region selected in this study to evaluate service verification is `Busan' in Korea. Busan adopted 16 services in 4 sections last year and begun demonstration to improve quality of life and resolve urban environment problems. In addition, Busan participated officially in `Global City Teams Challenge (GCTC)' held by National Institute of Standards and Technology (NIST) in USA last year and can be regarded as representative demonstration region in Korea. The result of survey showed that there were practical difficulties as explained below in the demonstration for resolving urban problems by applying smart technology. First, the participation for demonstration was low because citizens were either not aware or did not realize the demonstration. Second, after demonstrating various services at low cost, it resulted in less effect of service demonstration. Third, as functions get fused, it was found that management department, application criteria of technology and its process were ambiguous. In order to increase the efficiency of the demonstration for the rest of period through the result of this study, it is required to draw demand that citizens requires in order to raise public participation. In addition, it needs to focus more on services which are wanted to demonstrate rather than various service demonstrations. Lastly, it is necessary to build integration platform through cooperation

  3. Clinical Effectiveness of Different Polishing Systems and Self-Etch Adhesives in Class V Composite Resin Restorations: Two-Year Randomized Controlled Clinical Trial.

    Science.gov (United States)

    Jang, J-H; Kim, H-Y; Shin, S-M; Lee, C-O; Kim, D S; Choi, K-K; Kim, S-Y

    The aim of this randomized controlled clinical trial was to compare the clinical effectiveness of different polishing systems and self-etch adhesives in class V composite resin restorations. A total of 164 noncarious cervical lesions (NCCLs) from 35 patients were randomly allocated to one of four experimental groups, each of which used a combination of polishing systems and adhesives. The two polishing systems used were Sof-Lex XT (Sof), a multistep abrasive disc, and Enhance/Pogo (EP), a simplified abrasive-impregnated rubber instrument. The adhesive systems were Clearfil SE bond (CS), a two-step self-etch adhesive, and Xeno V (XE), a one-step self-etch adhesive. All NCCLs were restored with light-cured microhybrid resin composites (Z250). Restorations were evaluated at baseline and at 6, 12, 18, and 24 months by two blinded independent examiners using modified FDI criteria. The Fisher exact test and generalized estimating equation analysis considering repeated measurements were performed to compare the outcomes between the polishing systems and adhesives. Three restorations were dislodged: two in CS/Sof and one in CS/EP. None of the restorations required any repair or retreatment except those showing retention loss. Sof was superior to EP with regard to surface luster, staining, and marginal adaptation (p0.05). Sof is clinically superior to EP for polishing performance in class V composite resin restoration. XE demonstrates clinically equivalent bonding performance to CS.

  4. The mixed waste landfill integrated demonstration

    International Nuclear Information System (INIS)

    Burford, T.D.; Williams, C.V.

    1994-01-01

    The Mixed Waste Landfill Integrated Demonstration (MWLID) focuses on ''in-situ'' characterization, monitoring, remediation, and containment of landfills in arid environments that contain hazardous and mixed waste. The MWLID mission is to assess, demonstrate, and transfer technologies and systems that lead to faster, better, cheaper, and safer cleanup. Most important, the demonstrated technologies will be evaluated against the baseline of conventional technologies and systems. The comparison will include the cost, efficiency, risk, and feasibility of using these innovative technologies at other sites

  5. Test and Demonstration Assets of New Mexico

    Energy Technology Data Exchange (ETDEWEB)

    None

    2008-03-31

    This document was developed by the Arrowhead Center of New Mexico State University as part of the National Security Preparedness Project (NSPP), funded by a DOE/NNSA grant. The NSPP has three primary components: business incubation, workforce development, and technology demonstration and validation. The document contains a survey of test and demonstration assets in New Mexico available for external users such as small businesses with security technologies under development. Demonstration and validation of national security technologies created by incubator sources, as well as other sources, are critical phases of technology development. The NSPP will support the utilization of an integrated demonstration and validation environment.

  6. FY-94 buried waste integrated demonstration program report

    International Nuclear Information System (INIS)

    1994-01-01

    The Buried Waste Integrated Demonstration (BWID) supports the applied research, development, demonstration, and evaluation of a multitude of advanced technologies. These technologies are being integrated to form a comprehensive remediation system for the effective and efficient remediation of buried waste. These efforts are identified and coordinated in support of the U.S. Department of Energy (DOE), Environmental Restoration and Waste Management (ER/WM) needs and objectives. This document summarizes previous demonstrations and describes the FY-94 BWID technology development and demonstration activities. Sponsored by the DOE Office of Technology Development (OTD), BWID works with universities and private industry to develop these technologies, which are being transferred to the private sector for use nationally and internationally. A public participation policy has been established to provide stakeholders with timely and accurate information and meaningful opportunities for involvement in the technology development and demonstration process

  7. Buried Waste Integrated Demonstration commercialization actions plans. Volume 1

    Energy Technology Data Exchange (ETDEWEB)

    Kaupanger, R.M. [EG and G Idaho, Inc., Idaho Falls, ID (United States); Glore, D. [Advanced Sciences, Inc. (United States)

    1994-04-01

    The Buried Waste Integrated Demonstration (BWID) is sponsored by US Department of Energy (DOE) Office of Technology Development. BWID supports the development and demonstration of a suite of technologies that when integrated with commercially available baseline technologies form a comprehensive system for the effective and efficient remediation of buried waste throughout the DOE complex. BWID evaluates, validates, and demonstrates technologies and transfers this information throughout DOE and private industry to support DOE. remediation planning and implementation activities. This report documents commercialization action plans for five technologies with near-term commercialization/ implementation potential as well as provides a status of commercial and academic partners for each technology.

  8. THE IMPLEMENTATION OF ORAL PRESENTATION IN PROMOTING LEARNER AUTONOMY IN CLASS

    Directory of Open Access Journals (Sweden)

    Rahmatika Kayyis

    2017-02-01

    Full Text Available In class of Phonetic phonology, students having confusion about a huge differences of each symbol and its phoneme between Indonesia and English. Only by giving them a lecturer in each meeting would not satisfy their needs. As it simply cannot keep up with it, learners have to be able to process and investigate their curiosity on their own autonomously.  Oral presentation is believed as one of implementations to promote learner autonomy in University environment. 32 students in the class divided into 10 groups presented several topics about phonetic and phonology. The data were collected through observation, interview, and questionaire. Then, they were analyzed using the steps in case study research method. The finding shows that oral presentation had given them “freedom”to discover and autonomy to do their own learning. They also given good response relating of  what they had learnt dan investigated during teaching learning in Phonetic and Phonology Class.

  9. A randomized controlled 30 years follow up of three conventional resin composites in Class II restorations

    DEFF Research Database (Denmark)

    Pallesen, Ulla; van Dijken, Jan WV

    2015-01-01

    Objective. The aim of this 30 year randomized controlled study was to evaluate, by intrain-dividual comparisons, the durability of three conventional resin composites in Class IIrestorations. Methods. Each of 30 participants, 21 female and 9 male (mean age 30 years, range 20–43),received at least...... three (one set) as similar as possible Class II restorations of moderate size.After cavity preparation, the three cavities were chosen at random to be restored with twochemical-cured (P10, Miradapt) and one light-cured resin composite (P30). A chemical-curedenamel bonding agent was applied after etching...... resin composites (p = 0.45).The variables tooth type, cavity size, age, and gender of the participants did not significantlyaffect the probability of failure. Significance. The three conventional resin composites showed good clinical performance dur-ing the 30 year evaluation. The chemical cured resin...

  10. A randomized controlled 27 years follow up of three resin composites in Class II restorations

    DEFF Research Database (Denmark)

    Pallesen, Ulla; van Dijken, Jan WV

    2015-01-01

    Objective: To evaluate the durability of three conventional resin composites in Class II restorations during 27 years. Methods: Thirty participants, 25 female and 5 male (mean age 38.2 years, range 25–63), received at least three (one set) as similar as possible Class II restorations of moderate...... size. The three cavities were chosen at random to be restored with a chemical-cured (Clearfil Posterior) and two visible light-cured resin composites (Adaptic II, Occlusin). A chemical-cured enamel bonding agent (Clearfil New Bond) was applied after Ca(OH)2 covering of dentin and enamel etch. Marginal......: Class II restorations of the three conventional resin composites showed an acceptable success rate during the 27 year evaluation....

  11. Final Comparison Study of Teaching Blended In-Class Courses vs. Teaching Distance Education Courses

    Directory of Open Access Journals (Sweden)

    Susan J. Martin

    2012-12-01

    Full Text Available This paper will share with the members of the conference the findings from the final study. This study contains five semesters of analyzed data which compares the retention of students, final grades for students, grades for five specific tasks that were given in blended in-class courses and in the totally online courses, and a comparison of data by GPA, gender, and by class level. All courses were American Politics PLSC 111. Each semester one or two American Politics courses were conducted in the classroom and one American Politics distance education course was conducted totally online. Each time the courses were given, it was during the same semester and by the same professor who is the researcher.

  12. The effect of fiber placement or flowable resin lining on microleakage in Class II adhesive restorations.

    Science.gov (United States)

    Belli, Sema; Orucoglu, Hasan; Yildirim, Cihan; Eskitascioglu, Gürcan

    2007-04-01

    The aim of this in vitro study was to evaluate the effect of two fibers (polyethylene or glass) and a flowable resin liner on microleakage in Class II adhesive restorations. Class II adhesive cavities were prepared on mesial and distal surfaces of 40 extracted sound human molars. The cavity margins were below or above the CEJ. The teeth were randomly divided into four groups according to the restoration technique: group 1: restored with a resin composite (AP-X, Kuraray) in bulk after SE Bond (Kuraray) treatment; group 2: flowable resin liner (Protect Liner F, Kuraray) was used before composite restoration; in group 3, a polyethylene fiber (Ribbond) and in group 4, a glass fiber (everStick NET, StickTech) was placed into the bed of flowable resin before composite restoration. Samples were finished, stored in distilled water for 7 days at room temperature, and then thermocycled for 300 cycles between 5 degrees C and 55 degrees C. After sealing the apices, the teeth were varnished within 1 mm of the margins and placed in 0.5% basic fuchsin dye for 24 h at 37 degrees C. After rinsing, the teeth were sectioned longitudinally through the restorations and microleakage was evaluated with a stereomicroscope. Marginal penetration was scored on a 0 to 4 scale, and the data were statistically analyzed using Kruskal-Wallis and the Mann-Whitney U-test. Flowable resin, everStick NET, and Ribbond THM used in combination with flowable resin significantly reduced leakage at occlusal margins in cavities with enamel margins (p 0.05). Use of flowable composite alone or in combination with polyethylene or glass fibers reduces occlusal leakage in Class II adhesive cavities with enamel margins.

  13. Microleakage in Class II composite restorations with margins below the CEJ: In vitro evaluation of different restorative techniques

    Science.gov (United States)

    Chiesa, Marco; Scribante, Andrea; Mekler, Jenia; Colombo, Marco

    2013-01-01

    Objectives: The purpose of this in vitro study was to evaluate the microleakage in “deep” Class II composite restorations with gingival cavosurface margin below the CEJ (cemento-enamel junction) and restored with different techniques. Study Design: Fifty human teeth were used. In each tooth two standardized Class II slot cavities (on mesial and on distal surfaces) were prepared: the buccolingual extension of the cavities was 4 mm; the gingival wall was located in dentin/cementum (2 mm beyond the CEJ). The prepared teeth were randomly assigned to 5 experimental groups (of 10 specimens and 20 cavities each) and restored. Group 1: Filtek TM Supreme XTE Flowable (3MESPE) + Universal Filtek Supreme XTE (3MESPE), Group 2: GrandioSO Heavy Flow (Voco) + GrandioSo (Voco), Group 3: SDR™ (Dentsply Caulk) + Esthet-X® HD (Dentsply Caulk), Group 4: SonicFill (Kerr), Group 5: Grandio (Voco). After thermocycling, the specimens were immersed in a 0.5% basic fuchsine dye solution and incubated at 37°C for 24 hours. The teeth were subsequently sectioned mesiodistally. All specimens were examined at 25× in a stereomicroscope and standardized digital images were obtained. Dye penetration was measured from gingival margins. Results: The results demonstrated no significant leakage differences between Group 4 and Group 5, that both showed significantly higher frequency distribution of Score 0. Group 2 and Group 3 showed a significant prevalence of Score 1, whereas Group 1 showed significantly higher frequency of Score 2. Conclusions: None of the restorative techniques tested completely eliminated microleakage dye penetration in dentin margins; marginal adaptation in Class II composite restorations with gingival wall below the CEJ varied in both substrates and from different restorative techniques used. Key words:Microleakage, Class II composite restorations, CEJ. PMID:23722121

  14. Hydrogen and Palladium Foil: Two Classroom Demonstrations

    Science.gov (United States)

    Klotz, Elsbeth; Mattson, Bruce

    2009-01-01

    In these two classroom demonstrations, students observe the reaction between H[subscript 2] gas and Pd foil. In the first demonstration, hydrogen and palladium combine within one minute at 1 atm and room temperature to yield the non-stoichiometric, interstitial hydride with formula close to the maximum known value, PdH[subscript 0.7]. In the…

  15. 40 CFR 1042.815 - Demonstrating availability.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 32 2010-07-01 2010-07-01 false Demonstrating availability. 1042.815 Section 1042.815 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR POLLUTION... Provisions for Remanufactured Marine Engines § 1042.815 Demonstrating availability. (a) A certified...

  16. Professor's Page: Do Demonstration Lessons Work?

    Science.gov (United States)

    Clarke, Doug

    2011-01-01

    As part of a large research and professional development project funded by the Catholic Education Office Melbourne (CEOM), called "Contemporary Teaching and Learning of Mathematics," the ACU team has been leading demonstration lessons. There is certainly not universal agreement on the worth of demonstration lessons in the mathematics…

  17. Status of IFR fuel cycle demonstration

    International Nuclear Information System (INIS)

    Lineberry, M.J.; Phipps, R.D.; McFarlane, H.F.

    1993-01-01

    The next major step in Argonne's Integral Fast Reactor (IFR) Program is demonstration of the pyroprocess fuel cycle, in conjunction with continued operation of EBR-II. The Fuel Cycle Facility (FCF) is being readied for this mission. This paper will address the status of facility systems and process equipment, the initial startup experience, and plans for the demonstration program

  18. Buried waste integrated demonstration technology integration process

    International Nuclear Information System (INIS)

    Ferguson, J.S.; Ferguson, J.E.

    1992-04-01

    A Technology integration Process was developed for the Idaho National Energy Laboratories (INEL) Buried Waste Integrated Demonstration (BWID) Program to facilitate the transfer of technology and knowledge from industry, universities, and other Federal agencies into the BWID; to successfully transfer demonstrated technology and knowledge from the BWID to industry, universities, and other Federal agencies; and to share demonstrated technologies and knowledge between Integrated Demonstrations and other Department of Energy (DOE) spread throughout the DOE Complex. This document also details specific methods and tools for integrating and transferring technologies into or out of the BWID program. The document provides background on the BWID program and technology development needs, demonstrates the direction of technology transfer, illustrates current processes for this transfer, and lists points of contact for prospective participants in the BWID technology transfer efforts. The Technology Integration Process was prepared to ensure compliance with the requirements of DOE's Office of Technology Development (OTD)

  19. Radiation safety at the West Valley Demonstration Project

    International Nuclear Information System (INIS)

    Hoffman, R.L.

    1997-01-01

    This is a report on the Radiation Safety Program at the West Valley Demonstration Project (WVDP). This Program covers a number of activities that support high-level waste solidification, stabilization of facilities, and decontamination and decommissioning activities at the Project. The conduct of the Program provides confidence that all occupational radiation exposures received during operational tasks at the Project are within limits, standards, and program requirements, and are as low as reasonably achievable

  20. Occlusal stability in Class II, Division 1, deep bite cases followed up for many years after orthodontic treatment.

    Science.gov (United States)

    Kondo, E

    1998-12-01

    This case report analyzes long-term occlusal stability that can be achieved in Class II, Division 1, deep bite cases with active treatment finished during the period of maxillomandibular growth. The analysis was designed to identify occlusal features common to two cases at the end of active treatment and to study how the occlusion changed with growth and jaw movement to achieve stability. The following occlusal features were shared by the two cases at the end of active treatment: (1) AB plane and axes of the maxillary and mandibular posterior teeth were perpendicular to functional occlusal plane; (2) the axis of the lower incisor was almost perpendicular to DC-L1i line; (3) the anterior occlusion was overcorrected to or near an edge-to-edge relationship. Items 1 and 2 remained unchanged throughout the follow-up periods, regardless of growth status, and the overjet and overbite increased during maxillomandibular growth after treatment. During the period of mandibular growth alone, after the end of retention, the axes of maxillary incisors tipped labially; as a result, F line became parallel to CDM line by the end of growth. The labial tipping of maxillary incisors brought the lower incisal edge into contact with or extremely near the inflection point (Bp).1 By the end of growth, the tangent of Bp became parallel to or coincident with DC-L1i line and perpendicular to the axis of the lower incisor, and the DC-L1i lines at various times posttreatment were almost parallel to each other in the two cases. Overjet increased as the maxillary incisors tipped labially, providing proper protrusive and retrusive paths for mandibular guidance. The angle between the functional occlusal plane and CDM line stayed almost the same as at the end of active treatment in the two cases, suggesting a possible change in the angle of eminence in harmony with the functional occlusal plane. These factors apparently contributed to the long-term occlusal stability in the two cases.

  1. Discovery of (1R,6S)-5-[4-(1-cyclobutyl-piperidin-4-yloxy)-phenyl]-3,4-diaza-bicyclo[4.1.0]hept-4-en-2-one (R,S-4a): histamine H(3) receptor inverse agonist demonstrating potent cognitive enhancing and wake promoting activity.

    Science.gov (United States)

    Hudkins, Robert L; Josef, Kurt A; Becknell, Nadine C; Aimone, Lisa D; Lyons, Jacquelyn A; Mathiasen, Joanne R; Gruner, John A; Raddatz, Rita

    2014-03-01

    A series of fused cyclopropyl-4,5-dihydropyridazin-3-one (3,4-diaza-bicyclo[4.1.0]hept-4-en-2-one) phenoxypiperidine analogs was designed and synthesized, leading to the identification of (1R,6S)-5-[4-(1-cyclobutyl-piperidin-4-yloxy)-phenyl]-3,4-diaza-bicyclo[4.1.0]hept-4-en-2-one (R,S-4a) as a second-generation pyridazin-3-one H3R antagonist. Compound R,S-4a was a potent H3R functional antagonist in vivo in the rat dipsogenia model, demonstrated potent wake activity in the rat EEG/EMG model, and enhanced short-term memory in the rat social recognition memory model at doses as low as 0.03-0.3 mg/kg po. Copyright © 2014 Elsevier Ltd. All rights reserved.

  2. Marketing Plan for Demonstration and Validation Assets

    Energy Technology Data Exchange (ETDEWEB)

    None, None

    2008-05-30

    The National Security Preparedness Project (NSPP), is to be sustained by various programs, including technology demonstration and evaluation (DEMVAL). This project assists companies in developing technologies under the National Security Technology Incubator program (NSTI) through demonstration and validation of technologies applicable to national security created by incubators and other sources. The NSPP also will support the creation of an integrated demonstration and validation environment. This report documents the DEMVAL marketing and visibility plan, which will focus on collecting information about, and expanding the visibility of, DEMVAL assets serving businesses with national security technology applications in southern New Mexico.

  3. Allergy immunotherapy across the life cycle to promote active and healthy ageing: from research to policies: An AIRWAYS Integrated Care Pathways (ICPs) programme item (Action Plan B3 of the European Innovation Partnership on active and healthy ageing) and the Global Alliance against Chronic Respiratory Diseases (GARD), a World Health Organization GARD research demonstration project.

    Science.gov (United States)

    Calderon, M A; Demoly, P; Casale, T; Akdis, C A; Bachert, C; Bewick, M; Bilò, B M; Bohle, B; Bonini, S; Bush, A; Caimmi, D P; Canonica, G W; Cardona, V; Chiriac, A M; Cox, L; Custovic, A; De Blay, F; Devillier, P; Didier, A; Di Lorenzo, G; Du Toit, G; Durham, S R; Eng, P; Fiocchi, A; Fox, A T; van Wijk, R Gerth; Gomez, R M; Haathela, T; Halken, S; Hellings, P W; Jacobsen, L; Just, J; Tanno, L K; Kleine-Tebbe, J; Klimek, L; Knol, E F; Kuna, P; Larenas-Linnemann, D E; Linneberg, A; Matricardi, M; Malling, H J; Moesges, R; Mullol, J; Muraro, A; Papadopoulos, N; Passalacqua, G; Pastorello, E; Pfaar, O; Price, D; Del Rio, P Rodriguez; Ruëff, R; Samolinski, B; Scadding, G K; Senti, G; Shamji, M H; Sheikh, A; Sisul, J C; Sole, D; Sturm, G J; Tabar, A; Van Ree, R; Ventura, M T; Vidal, C; Varga, E M; Worm, M; Zuberbier, T; Bousquet, J

    2016-01-01

    Allergic diseases often occur early in life and persist throughout life. This life-course perspective should be considered in allergen immunotherapy. In particular it is essential to understand whether this al treatment may be used in old age adults. The current paper was developed by a working group of AIRWAYS integrated care pathways for airways diseases, the model of chronic respiratory diseases of the European Innovation Partnership on active and healthy ageing (DG CONNECT and DG Santé). It considered (1) the political background, (2) the rationale for allergen immunotherapy across the life cycle, (3) the unmet needs for the treatment, in particular in preschool children and old age adults, (4) the strategic framework and the practical approach to synergize current initiatives in allergen immunotherapy, its mechanisms and the concept of active and healthy ageing.

  4. Codes and standards research, development and demonstration roadmap

    Energy Technology Data Exchange (ETDEWEB)

    None, None

    2008-07-22

    C&S RD&D Roadmap - 2008: This Roadmap is a guide to the Research, Development & Demonstration activities that will provide data required for Standards Development Organizations (SDOs) to develop performance-based codes and standards for a commercial hydrogen fueled transportation sector in the U.S.

  5. Demonstration Aids for Aviation Education [National Aviation Education Workshop].

    Science.gov (United States)

    Federal Aviation Administration (DOT), Washington, DC.

    This manual, compiled by a Committee of the Curriculum Laboratory of the Civil Air Patrol, contains 105 demonstrations and activities which can be used to introduce the elementary student to the properties of air as related to aviation, what makes airplanes fly, and the role of weather in aviation. (CP)

  6. Trajectory learning from human demonstrations via manifold mapping

    CSIR Research Space (South Africa)

    Hiratsuka, M

    2016-10-01

    Full Text Available This work proposes a framework that enables arbitrary robots with unknown kinematics models to imitate human demonstrations to acquire a skill, and reproduce it in real-time. The diversity of robots active in non-laboratory environments is growing...

  7. Irreversible Wash Aid Additive for Cesium Mitigation: WARRP Demonstration

    Energy Technology Data Exchange (ETDEWEB)

    Kaminski, Michael [Argonne National Lab. (ANL), Argonne, IL (United States)

    2015-01-01

    This activity demonstrated, on a practical scale, the primary unit operations for building a containment structure for radioactive wash waters, washing down a hypothetically radioactively contaminated vehicle, collecting the hypothetically radioactive slurry waste water, filtering the hypothetically radioactive wash waters, disassembling the containment, and transporting the materials for final disposition.

  8. Demonstration and Deployment Strategy Workshop: Summary

    Energy Technology Data Exchange (ETDEWEB)

    none,

    2014-05-01

    This report is based on the proceedings of the U.S. Department of Energy Bioenergy Technologies Office Demonstration and Deployment Strategy Workshop, held on March 12–13, 2014, at Argonne National Laboratory.

  9. Optical Communications and Sensor Demonstration (OCSD)

    Data.gov (United States)

    National Aeronautics and Space Administration — The OCSD mission will demonstrate high-speed optical transmission of data using a miniature laser communications system that will increase downlink data rates for...

  10. A Facile and Effective Chemiluminescence Demonstration Experiment

    Science.gov (United States)

    Mohan, Arthur G.; Turro, Nicholas J.

    1974-01-01

    Describes a chemiluminescence system which can be used to demonstrate the effects of certain factors which affect the rate of reaction (temperature, concentration, catalysis, solvent, etc.), and to perform experiments relevant to the mechanism of the system. (SLH)

  11. CubeSat Deformable Mirror Demonstration

    Data.gov (United States)

    National Aeronautics and Space Administration — The goal of the CubeSat Deformable Mirror Demonstration is to characterize the performance of a small deformable mirror over a year in low-Earth orbit. Small form...

  12. Hybrid Life Support System Technology Demonstrations

    Science.gov (United States)

    Morrow, R. C.; Wetzel, J. P.; Richter, R. C.

    2018-02-01

    Demonstration of plant-based hybrid life support technologies in deep space will validate the function of these technologies for long duration missions, such as Mars transit, while providing dietary variety to improve habitability.

  13. Keys to Successful EPIQ Business Demonstrator Implementation

    NARCIS (Netherlands)

    Shoikova, Elena; Denishev, Vladislav

    2009-01-01

    Shoikova, E., & Denishev, V. (2009). Keys to Successful EPIQ Business Demonstrator Implementation. Paper presented at the 'Open workshop of TENCompetence - Rethinking Learning and Employment at a Time of Economic Uncertainty-event'. November, 19, 2009, Manchester, United Kingdom: TENCompetence.

  14. 2017 SmartWay Logistics Tool Demonstration

    Science.gov (United States)

    This EPA presentation provides information on the SmartWay Logistics Carrier Tool: its background and development, participation in the program, application process, emission metrics, tool demonstration, data collection, and schedule for 2017.

  15. A Demonstration of Automated DNA Sequencing.

    Science.gov (United States)

    Latourelle, Sandra; Seidel-Rogol, Bonnie

    1998-01-01

    Details a simulation that employs a paper-and-pencil model to demonstrate the principles behind automated DNA sequencing. Discusses the advantages of automated sequencing as well as the chemistry of automated DNA sequencing. (DDR)

  16. Medicare Demonstration Projects and Evaluation Reports

    Data.gov (United States)

    U.S. Department of Health & Human Services — The Centers for Medicare and Medicaid Services (CMS) conducts and sponsors a number of innovative demonstration projects to test and measure the effect of potential...

  17. Lessons Learned from Microgrid Demonstrations Worldwide

    Energy Technology Data Exchange (ETDEWEB)

    Marnay, Chris [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Zhou, Nan [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Qu, Min [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Romankiewicz, John [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)

    2012-01-31

    The survey leads to policy recommendations for starting a microgrid demonstration program and overall development of microgrid and distributed energy. Additionally, specific recommendations have been made for China specifically.

  18. Classroom Demonstrations in Materials Science/Engineering.

    Science.gov (United States)

    Hirschhorn, J. S.; And Others

    Examples are given of demonstrations used at the University of Wisconsin in a materials science course for nontechnical students. Topics include crystal models, thermal properties, light, and corrosion. (MLH)

  19. Edison Demonstration of Smallsat Networks (EDSN)

    Data.gov (United States)

    National Aeronautics and Space Administration — NASA's Edison Demonstration of SmallSat Networks (EDSN) mission will launch and deploy a group of eight cubesats into a loose formation approximately 250 miles (400...

  20. Three Inexpensive Static-Electricity Demonstrations.

    Science.gov (United States)

    Gore, Gordon R.; Gregg, William R.

    1992-01-01

    Describes demonstrations to (1) construct an inexpensive static electricity detector; (2) obtain an abundant supply of either negative or positive charge using household items; and (3) create static electricity using a Tesla coil or Van de Graaff generator. (MDH)

  1. Integrated Ground Operations Demonstration Units Project

    Data.gov (United States)

    National Aeronautics and Space Administration — The overall goal of the project is to demonstrate cost efficient cryogenic operations on a relevant scale that can be projected onto future Spaceport architectures...

  2. Nuclear Systems (NS): Technology Demonstration Unit (TDU)

    Data.gov (United States)

    National Aeronautics and Space Administration — The Nuclear Systems Project demonstrates nuclear power technology readiness to support the goals of NASA's Space Technology Mission Directorate. To this end, the...

  3. Reliability demonstration of imaging surveillance systems

    International Nuclear Information System (INIS)

    Sheridan, T.F.; Henderson, J.T.; MacDiarmid, P.R.

    1979-01-01

    Security surveillance systems which employ closed circuit television are being deployed with increasing frequency for the protection of property and other valuable assets. A need exists to demonstrate the reliability of such systems before their installation to assure that the deployed systems will operate when needed with only the scheduled amount of maintenance and support costs. An approach to the reliability demonstration of imaging surveillance systems which employ closed circuit television is described. Failure definitions based on industry television standards and imaging alarm assessment criteria for surveillance systems are discussed. Test methods which allow 24 hour a day operation without the need for numerous test scenarios, test personnel and elaborate test facilities are presented. Existing reliability demonstration standards are shown to apply which obviate the need for elaborate statistical tests. The demonstration methods employed are shown to have applications in other types of imaging surveillance systems besides closed circuit television

  4. First Demonstration of Pulsar Positioning System (PPS)

    Data.gov (United States)

    National Aeronautics and Space Administration — Carry out first time demonstration of a global positioning system using pulsars as celestial clocks. Pulsars are rotating neutron stars that emit clock-like pulses...

  5. Some simple demonstration experiments involving homopolar motors

    OpenAIRE

    Stewart,Seán M.

    2007-01-01

    The ready availability of very strong permanent magnets in the form of rare-earth magnetic alloys such as neodymium-iron-boron has lead to renewed interest in one of the oldest types of electric motors - the homopolar motor. The ease with which a demonstration homopolar motor can now be built and operated when neodymium magnets are used is quite remarkable. In this paper some simple homopolar motors employing neodymium magnets suitable for demonstrational purposes are described and discussed.

  6. The MAJORANA DEMONSTRATOR Neutrinoless Double-Beta Decay Experiment

    Directory of Open Access Journals (Sweden)

    N. Abgrall

    2014-01-01

    Full Text Available The Majorana Demonstrator will search for the neutrinoless double-beta (ββ0ν decay of the isotope Ge with a mixed array of enriched and natural germanium detectors. The observation of this rare decay would indicate that the neutrino is its own antiparticle, demonstrate that lepton number is not conserved, and provide information on the absolute mass scale of the neutrino. The Demonstrator is being assembled at the 4850-foot level of the Sanford Underground Research Facility in Lead, South Dakota. The array will be situated in a low-background environment and surrounded by passive and active shielding. Here we describe the science goals of the Demonstrator and the details of its design.

  7. Hardware demonstration of high-speed networks for satellite applications.

    Energy Technology Data Exchange (ETDEWEB)

    Donaldson, Jonathon W.; Lee, David S.

    2008-09-01

    This report documents the implementation results of a hardware demonstration utilizing the Serial RapidIO{trademark} and SpaceWire protocols that was funded by Sandia National Laboratories (SNL's) Laboratory Directed Research and Development (LDRD) office. This demonstration was one of the activities in the Modeling and Design of High-Speed Networks for Satellite Applications LDRD. This effort has demonstrated the transport of application layer packets across both RapidIO and SpaceWire networks to a common downlink destination using small topologies comprised of commercial-off-the-shelf and custom devices. The RapidFET and NEX-SRIO debug and verification tools were instrumental in the successful implementation of the RapidIO hardware demonstration. The SpaceWire hardware demonstration successfully demonstrated the transfer and routing of application data packets between multiple nodes and also was able reprogram remote nodes using configuration bitfiles transmitted over the network, a key feature proposed in node-based architectures (NBAs). Although a much larger network (at least 18 to 27 nodes) would be required to fully verify the design for use in a real-world application, this demonstration has shown that both RapidIO and SpaceWire are capable of routing application packets across a network to a common downlink node, illustrating their potential use in real-world NBAs.

  8. Orbital Express fluid transfer demonstration system

    Science.gov (United States)

    Rotenberger, Scott; SooHoo, David; Abraham, Gabriel

    2008-04-01

    Propellant resupply of orbiting spacecraft is no longer in the realm of high risk development. The recently concluded Orbital Express (OE) mission included a fluid transfer demonstration that operated the hardware and control logic in space, bringing the Technology Readiness Level to a solid TRL 7 (demonstration of a system prototype in an operational environment). Orbital Express (funded by the Defense Advanced Research Projects Agency, DARPA) was launched aboard an Atlas-V rocket on March 9th, 2007. The mission had the objective of demonstrating technologies needed for routine servicing of spacecraft, namely autonomous rendezvous and docking, propellant resupply, and orbital replacement unit transfer. The demonstration system used two spacecraft. A servicing vehicle (ASTRO) performed multiple dockings with the client (NextSat) spacecraft, and performed a variety of propellant transfers in addition to exchanges of a battery and computer. The fluid transfer and propulsion system onboard ASTRO, in addition to providing the six degree-of-freedom (6 DOF) thruster system for rendezvous and docking, demonstrated autonomous transfer of monopropellant hydrazine to or from the NextSat spacecraft 15 times while on orbit. The fluid transfer system aboard the NextSat vehicle was designed to simulate a variety of client systems, including both blowdown pressurization and pressure regulated propulsion systems. The fluid transfer demonstrations started with a low level of autonomy, where ground controllers were allowed to review the status of the demonstration at numerous points before authorizing the next steps to be performed. The final transfers were performed at a full autonomy level where the ground authorized the start of a transfer sequence and then monitored data as the transfer proceeded. The major steps of a fluid transfer included the following: mate of the coupling, leak check of the coupling, venting of the coupling, priming of the coupling, fluid transfer, gauging

  9. High School Pedagogy: The Influence of High School In-class Activities and Events On Introductory College Physics Success

    OpenAIRE

    Carter, Brooke

    2014-01-01

    This study explores how students’ grades in introductory college physics are influenced by the pedagogy used in their high school physics classes. The success of college science professors is often judged on the basis of the success of their students. This disregards the 18+ years of experiences with which students come into their physics classroom. This study aims to answer the question of what pedagogy best prepares students for introductory college physics. This quantitative study analyzes...

  10. Decision support software technology demonstration plan

    Energy Technology Data Exchange (ETDEWEB)

    SULLIVAN,T.; ARMSTRONG,A.

    1998-09-01

    The performance evaluation of innovative and alternative environmental technologies is an integral part of the US Environmental Protection Agency's (EPA) mission. Early efforts focused on evaluating technologies that supported the implementation of the Clean Air and Clean Water Acts. In 1986 the Agency began to demonstrate and evaluate the cost and performance of remediation and monitoring technologies under the Superfund Innovative Technology Evaluation (SITE) program (in response to the mandate in the Superfund Amendments and Reauthorization Act of 1986 (SARA)). In 1990, the US Technology Policy was announced. This policy placed a renewed emphasis on making the best use of technology in achieving the national goals of improved quality of life for all Americans, continued economic growth, and national security. In the spirit of the technology policy, the Agency began to direct a portion of its resources toward the promotion, recognition, acceptance, and use of US-developed innovative environmental technologies both domestically and abroad. Decision Support Software (DSS) packages integrate environmental data and simulation models into a framework for making site characterization, monitoring, and cleanup decisions. To limit the scope which will be addressed in this demonstration, three endpoints have been selected for evaluation: Visualization; Sample Optimization; and Cost/Benefit Analysis. Five topics are covered in this report: the objectives of the demonstration; the elements of the demonstration plan; an overview of the Site Characterization and Monitoring Technology Pilot; an overview of the technology verification process; and the purpose of this demonstration plan.

  11. High energy laser demonstrators for defense applications

    Science.gov (United States)

    Jung, M.; Riesbeck, Th.; Schmitz, J.; Baumgärtel, Th.; Ludewigt, K.; Graf, A.

    2017-01-01

    Rheinmetall Waffe Munition has worked since 30 years in the area of High Energy Laser (HEL) for defence applications, starting from pulsed CO2 to pulsed glass rods lasers. In the last decade Rheinmetall Waffe Munition changed to diode pumped solid state laser (DPSSL) technology and has successfully developed, realised and tested a variety of versatile HEL weapon demonstrators for air- and ground defence scenarios like countering rocket, artillery, mortar, missile (RAMM), unmanned aerial systems (UAS) and unexploded ordnances clearing. By employing beam superimposing technology and a modular laser weapon concept, the total optical power has been successively increased. Stationary weapon platforms, military vehicles and naval platforms have been equipped with high energy laser effectors. The contribution gives a summary of the most recent development stages of Rheinmetalls HEL weapon program. In addition to the stationary 30 kW laser weapon demonstrator, we present vehicle based HEL demonstrators: the 5 kW class Mobile HEL Effector Track V, the 20 kW class Mobile HEL Effector Wheel XX and the 50 kW class Mobile HEL Effector Container L and the latest 10 kW HEL effector integrated in the naval weapon platform MLG 27. We describe the capabilities of these demonstrators against different potential targets. Furthermore, we will show the capability of the 30 kW stationary Laser Weapon Demonstrator integrated into an existing ground based air defence system to defeat saturated attacks of RAMM and UAS targets.

  12. A demonstration device for cosmic rays telescopes

    Science.gov (United States)

    Esposito, Salvatore

    2018-01-01

    We describe a hands-on accurate demonstrator for cosmic rays realized by six high school students. The main aim is to show the relevance and the functioning of the principal parts of a cosmic ray telescope (muon detector), with the help of two large sized wooden artefacts. The first one points out how cosmic rays can be tracked in a muon telescope, while the other one shows the key avalanche process of electronic ionization that effectively allows muon detection through a photomultiplier. Incoming cosmic rays are visualized in terms of laser beams, whose 3D trajectory is highlighted by turning on LEDs on two orthogonal matrices. Instead the avalanche ionization process is demonstrated through the avalanche falling off glass marbles on an inclined plane, finally turning on a LED. A pictured poster accompanying the demonstrator is as effective in assisting cosmic ray demonstration and its detection. The success of the demonstrator has been fully proven by the general public during a science festival, in which the corresponding project won the Honorable Mention in a dedicated competition.

  13. Accelerated reliability demonstration under competing failure modes

    International Nuclear Information System (INIS)

    Luo, Wei; Zhang, Chun-hua; Chen, Xun; Tan, Yuan-yuan

    2015-01-01

    The conventional reliability demonstration tests are difficult to apply to products with competing failure modes due to the complexity of the lifetime models. This paper develops a testing methodology based on the reliability target allocation for reliability demonstration under competing failure modes at accelerated conditions. The specified reliability at mission time and the risk caused by sampling of the reliability target for products are allocated for each failure mode. The risk caused by degradation measurement fitting of the target for a product involving performance degradation is equally allocated to each degradation failure mode. According to the allocated targets, the accelerated life reliability demonstration test (ALRDT) plans for the failure modes are designed. The accelerated degradation reliability demonstration test plans and the associated ALRDT plans for the degradation failure modes are also designed. Next, the test plan and the decision rules for the products are designed. Additionally, the effects of the discreteness of sample size and accepted number of failures for failure modes on the actual risks caused by sampling for the products are investigated. - Highlights: • Accelerated reliability demonstration under competing failure modes is studied. • The method is based on the reliability target allocation involving the risks. • The test plan for the products is based on the plans for all the failure modes. • Both failure mode and degradation failure modes are considered. • The error of actual risks caused by sampling for the products is small enough

  14. RateMyProfessors.com versus formal in-class student evaluations of teaching

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    Theodore Coladarci

    2007-05-01

    Full Text Available Using data for 426 instructors at the University of Maine, we examined the relationship between RateMyProfessors.com (RMP indices and formal in-class student evaluations of teaching (SET. The two primary RMP indices correlate substantively and significantly with their respective SET items: RMP overall quality correlates r = .68 with SET item, Overall, how would you rate the instructor?; and RMP ease correlates r = .44 with SET item, How did the work load for this course compare to that of others of equal credit? Further, RMP overall quality and RMP ease each correlates with its corresponding SET factor derived from a principal components analysis of all 29 SET items: r = .57 and .51, respectively. While these RMP/SET correlations should give pause to those who are inclined to dismiss RMP indices as meaningless, the amount of variance left unexplained in SET criteria limits the utility of RMP. The ultimate implication of our results, we believe, is that higher education institutions should make their SET data publicly available online.

  15. Glenoid fossa position in Class III malocclusion associated with mandibular protrusion.

    Science.gov (United States)

    Innocenti, Cristina; Giuntini, Veronica; Defraia, Efisio; Baccetti, Tiziano

    2009-04-01

    Our aim in this study was to investigate the position of the glenoid fossa in subjects with Class III malocclusion associated with mandibular protrusion to better clarify the role of this craniofacial component in Class III skeletal disharmony. A sample of 30 subjects, aged 8 years +/- 6 months, with skeletal and dental Class III malocclusion associated with mandibular protrusion, normal skeletal vertical relationships, and normal mandibular dimensions, was compared with a control group of 33 subjects with skeletal and dental Class I relationships. The comparisons between the Class III group and the control group on the cephalometric measures for the assessment of glenoid fossa position were performed with the Mann-Whitney U test at P <0.05. Subjects with Class III malocclusion had a significantly more mesial position of the glenoid fossa, when compared with the control group as measured with 3 parameters. An anterior position of the glenoid fossa is a possible diagnostic anatomic feature of Class III malocclusion associated with mandibular protrusion. An effective measurement to evaluate glenoid fossa position in craniofacial relationships is the cephalometric distance from the glenoid fossa to the frontomaxillary-nasal suture.

  16. Evaluation of temporomandibular disorders in Class III patients treated with mandibular cervical headgear and fixed appliances.

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    Rey, Diego; Oberti, Giovanni; Baccetti, Tiziano

    2008-03-01

    Our aim in this study was to evaluate the prevalence of temporomandibular disorders (TMD) in Class III patients treated with mandibular cervical headgear (MCH) and fixed appliances. The sample of 75 patients included 25 patients with no previous orthodontic treatment, 25 Class I patients who had undergone orthodontic treatment with fixed appliances and without extractions, and 25 patients with dentoskeletal Class III disharmonies treated with MCH and fixed appliances. The Helkimo index was used to test the prevalence of TMD symptoms in the 3 groups. The prevalence rates of the Helkimo index in the 3 groups were compared with the z score on proportions. No statistically significant differences in the prevalence rates of the Helkimo index scores in the 3 groups were found (P = .367). Most subjects in the 3 groups had an Helkimo index of zero (66.7%). Subjects with Class III malocclusions treated with MCH and fixed appliances do not have greater prevalence of TMD symptoms than do Class I subjects treated with fixed appliances or untreated subjects.

  17. Improving attitudes toward mathematics learning with problem posing in class VIII

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    Vionita, Alfha; Purboningsih, Dyah

    2017-08-01

    This research is classroom action research which is collaborated to improve student's behavior toward math and mathematics learning at class VIII by using problem posing approach. The subject of research is all of students grade VIIIA which consist of 32 students. This research has been held on two period, first period is about 3 times meeting, and second period is about 4 times meeting. The instrument of this research is implementation of learning observation's guidance by using problem posing approach. Cycle test has been used to measure cognitive competence, and questionnaire to measure the students' behavior in mathematics learning process. The result of research shows the students' behavior has been improving after using problem posing approach. It is showed by the behavior's criteria of students that has increasing result from the average in first period to high in second period. Furthermore, the percentage of test result is also improve from 68,75% in first period to 78,13% in second period. On the other hand, the implementation of learning observation by using problem posing approach has also improving and it is showed by the average percentage of teacher's achievement in first period is 89,2% and student's achievement 85,8%. These results get increase in second period for both teacher and students' achievement which are 94,4% and 91,11%. As a result, students' behavior toward math learning process in class VIII has been improving by using problem posing approach.

  18. Online or In-Class: Evaluating an Alternative Online Pedagogy for Teaching Transcultural Nursing.

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    Ochs, Jessica H

    2017-06-01

    Online learning formats are prevalent in current higher education. Given the changing student demographics and the drive for creativity in educating a technology-savvy student, it is imperative to incorporate innovative and alternative learning modalities to engage these students. This pilot study was designed as a quality improvement program evaluation comparing the effects of an online learning module with traditional classroom delivery of transcultural nursing content using a posttest two-group survey design in associate degree nursing students. The students' perceived knowledge and confidence were investigated after receiving the lecture for both the online and in-class groups. Data analysis revealed the online cohort perceived themselves as more knowledgeable concerning the ways that cultural factors influence nursing care, but not more confident in providing culturally competent care. Due to the students' perceived knowledge gain, this pilot study supports the use of online learning modules as being more effective than the traditional classroom delivery of transcultural nursing content. [J Nurs Educ. 2017;56(6):368-372.]. Copyright 2017, SLACK Incorporated.

  19. A comparative study of treatment outcomes between begg and edgewise orthodontic systems in class I cases

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    Abas Ali Sahafian

    2014-01-01

    Full Text Available Background: The purpose of this study was to compare the occlusal peer assessment rating (PAR index in Class I patients treated by means of Begg and Edgewise methods. Materials and Methods: The pre- and post-treatment study models of Class I patients, referred to two private clinics, treated with Begg method (n = 30 and standard Edgewise method (n = 30, were reviewed retrospectively using PAR index including dental displacements, buccal occlusion (anteroposterior, vertical and transverse, overjet, overbite and midline parameters. The changes in PAR indices were analyzed using paired t-test. A P < 0.05 was considered as statistically significant. Results: There was no significant difference in sex and age distribution between the two groups. The improvement of buccal occlusion in patients treated by Begg method (1.51 ± 0.39 was significantly higher than that of patients treated by Edgewise method (0.28 ± 0.39. The duration of treatment in Begg method (17.8 ± 1.3 months was significantly shorter than that of Edgewise method (23.7 ± 1.3 months. However, there were no significant differences between the two methods in terms of total PAR index, dental displacements, overjet, overbite and midline. Conclusion: In conclusion, findings of the present study indicated that Begg method might be associated with better improvement of buccal occlusion and shorter duration of treatment

  20. Cephalometric changes in Class II division 1 patients treated with two maxillary premolars extraction

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    Marisana Piano Seben

    2013-08-01

    Full Text Available OBJECTIVE: The purpose of this study was to evaluate the cephalometric alterations in patients with Angle Class II division 1 malocclusion, orthodontically treated with extraction of two maxillary premolars. METHODS: The sample comprised 68 initial and final lateral cephalograms of 34 patients of both gender (mean initial age of 14.03 years and mean final age of 17.25 years, treated with full fixed appliances and extraction of the first maxillary premolars. In order to evaluate the alterations due the treatment between initial and final phases, the dependent t test was applied to the studied cephalometric variables. RESULTS: The dentoskeletal alterations due to extraction of two maxillary premolars in the Class II division 1 malocclusion were: maxillary retrusion, improvement of the maxillomandibular relation, increase of lower anterior face height, retrusion of the maxillary incisors, buccal inclination, protrusion and extrusion of the mandibular incisors, besides the reduction of overjet and overbite. The tissue alterations showed decrease of the facial convexity and retrusion of the upper lip. CONCLUSIONS: The extraction of two maxillary premolars in Class II division 1 malocclusion promotes dentoskeletal and tissue alterations that contribute to an improvement of the relation between the bone bases and the soft tissue profile.