WorldWideScience

Sample records for in-cell material-handling system

  1. Techniques for remote maintenance of in-cell material-handling system in the HFEF/N main cell

    International Nuclear Information System (INIS)

    Tobias, D.A.; Frickey, C.A.

    1975-01-01

    Operations in the main cell of HFEF/N have required development of remote handling equipment and unique techniques for maintaining the in-cell material-handling system. Specially designed equipment is used to remove a disabled crane or electromechanical manipulator bridge from its support rails and place it on floor stands for repair or maintenance. Support areas for the main cell, such as the spray chamber and hot repair area, provide essential decontamination, repair, and staging areas for the in-cell material-handling-system equipment and tools. A combined engineering and technical effort in upgrading existing master-slave manipulators has definitely reduced the requirements for their maintenance. The cell is primarily for postirradiation examination of LMFBR materials and fuel elements

  2. MHSS: a material handling system simulator

    Energy Technology Data Exchange (ETDEWEB)

    Pomernacki, L.; Hollstien, R.B.

    1976-04-07

    A Material Handling System Simulator (MHSS) program is described that provides specialized functional blocks for modeling and simulation of nuclear material handling systems. Models of nuclear fuel fabrication plants may be built using functional blocks that simulate material receiving, storage, transport, inventory, processing, and shipping operations as well as the control and reporting tasks of operators or on-line computers. Blocks are also provided that allow the user to observe and gather statistical information on the dynamic behavior of simulated plants over single or replicated runs. Although it is currently being developed for the nuclear materials handling application, MHSS can be adapted to other industries in which material accountability is important. In this paper, emphasis is on the simulation methodology of the MHSS program with application to the nuclear material safeguards problem. (auth)

  3. Cellular Manufacturing System with Dynamic Lot Size Material Handling

    Science.gov (United States)

    Khannan, M. S. A.; Maruf, A.; Wangsaputra, R.; Sutrisno, S.; Wibawa, T.

    2016-02-01

    Material Handling take as important role in Cellular Manufacturing System (CMS) design. In several study at CMS design material handling was assumed per pieces or with constant lot size. In real industrial practice, lot size may change during rolling period to cope with demand changes. This study develops CMS Model with Dynamic Lot Size Material Handling. Integer Linear Programming is used to solve the problem. Objective function of this model is minimizing total expected cost consisting machinery depreciation cost, operating costs, inter-cell material handling cost, intra-cell material handling cost, machine relocation costs, setup costs, and production planning cost. This model determines optimum cell formation and optimum lot size. Numerical examples are elaborated in the paper to ilustrate the characterictic of the model.

  4. Modelling dust liberation in bulk material handling systems

    NARCIS (Netherlands)

    Derakhshani, S.M.

    2016-01-01

    Dust has negative effects on the environmental conditions, human health as well as industrial equipment and processes. In this thesis, the transfer point of a belt conveyor as a bulk material handling system with a very high potential place for dust liberation is studied. This study is conducted

  5. Development and implementation of automated radioactive materials handling systems

    International Nuclear Information System (INIS)

    Jacoboski, D.L.

    1992-12-01

    Material handling of radioactive and hazardous materials has forced the need to pursue remotely operated and robotic systems in light of operational safety concerns. Manual maneuvering, repackaging, overpacking and inspecting of containers which store radioactive and hazardous materials is the present mode of operation at the Department of Energy (DOE) Fernald Environmental Management Project (FEMP) in Fernald Ohio. The manual methods are unacceptable in the eyes of concerned site workers and influential community oversight committees. As an example to respond to the FEMP material handling needs, design efforts have been initiated to provide a remotely operated system to repackage thousands of degradated drums containing radioactive Thorium: Later, the repackaged Thorium will be shipped offsite to a predesignated repository again requiring remote operation

  6. Radial Internal Material Handling System (RIMS) for Circular Habitat Volumes

    Science.gov (United States)

    Howe, Alan S.; Haselschwardt, Sally; Bogatko, Alex; Humphrey, Brian; Patel, Amit

    2013-01-01

    On planetary surfaces, pressurized human habitable volumes will require a means to carry equipment around within the volume of the habitat, regardless of the partial gravity (Earth, Moon, Mars, etc.). On the NASA Habitat Demonstration Unit (HDU), a vertical cylindrical volume, it was determined that a variety of heavy items would need to be carried back and forth from deployed locations to the General Maintenance Work Station (GMWS) when in need of repair, and other equipment may need to be carried inside for repairs, such as rover parts and other external equipment. The vertical cylindrical volume of the HDU lent itself to a circular overhead track and hoist system that allows lifting of heavy objects from anywhere in the habitat to any other point in the habitat interior. In addition, the system is able to hand-off lifted items to other material handling systems through the side hatches, such as through an airlock. The overhead system consists of two concentric circle tracks that have a movable beam between them. The beam has a hoist carriage that can move back and forth on the beam. Therefore, the entire system acts like a bridge crane curved around to meet itself in a circle. The novelty of the system is in its configuration, and how it interfaces with the volume of the HDU habitat. Similar to how a bridge crane allows coverage for an entire rectangular volume, the RIMS system covers a circular volume. The RIMS system is the first generation of what may be applied to future planetary surface vertical cylinder habitats on the Moon or on Mars.

  7. Improvement Of Physical Ergonomics Using Material Handling Systems

    Directory of Open Access Journals (Sweden)

    Naveen Kumar

    2017-01-01

    Full Text Available This research paper is an investigation of the physical ergonomics of the work place in an automotive parts manufacturing company . Material transfer from one station to another station was done by hand including a walk of a few steps to the next station. The unmachined components that has a quite heavy raw weight also they are being loaded and unloaded by hand .Due to this continuous practice some workers began complaining physical pain in their backs and muscular related pains. The work conditions of the workers were assessed using the REBA Rapid Entire Body Assessment test to understand the stress and the impact the work environment they are exposed to. Few material handling concepts have been suggested and explained to improve the quality of the work conditions for the workers and the REBA test tends to show some significant improvement when these improvements are implemented into the production line.

  8. SIMULASI GROUP TECHNOLOGY SYSTEM UNTUK MEMINIMALKAN BIAYA MATERIAL HANDLING DENGAN METODE HEURISTIC

    Directory of Open Access Journals (Sweden)

    Much. Djunaidi

    2006-04-01

    Full Text Available Group Technology System merupakan metode pengaturan fasilitas produksi (machine groups yang dibutuhkan untuk memproses suatu part family tertentu ke dalam sel-sel manufaktur. Pengaturan tata letak di CV. Sonytex yang berdasarkan process layout mengakibatkan perusahaan menghadapi permasalahan berupa tingginya kebutuhan material handling. Salah satu kriteria kinerja dalam pembentukan sel manufaktur pada GTS adalah meminimasi total jarak material handling, sehingga dapat mengurangi biaya material handling dan meningkatkan produktivitas. Dalam penelitian ini digunakan tiga metode, yaitu Bond Energy Algorithm (BEA, Rank Order Clustering (ROC dan Rank Order Clustering 2 (ROC2. Hasil dari penelitian ini adalah dengan menerapkan group technology systems diperoleh total pengurangan jarak material handling sebesar 70 m dan penghematan biaya material handling sebesar Rp 1.534.978,-. Berdasarkan model simulasi, relayout dengan metode BEA meningkatkan jumlah produksi sebesar 1 unit produk/hari dan penurunan waktu tunggu sebesar 0,575 menit.

  9. The use of physical model simulation to emulate an AGV material handling system

    International Nuclear Information System (INIS)

    Hurley, R.G.; Coffman, P.E.; Dixon, J.R.; Walacavage, J.G.

    1987-01-01

    This paper describes an application of physical modeling to the simulation of a prototype AGV (Automatic Guided Vehicle) material handling system. Physical modeling is the study of complex automated manufacturing and material handling systems through the use of small scale components controlled by mini and/or microcomputers. By modeling the mechanical operations of the proposed AGV material handling system, it was determined that control algorithms and AGV dispatch rules could be developed and evaluated. This paper presents a brief explanation of physical modeling as a simulation tool and addresses in detail the development of the control algorithm, dispatching rules, and a prototype physical model of a flexible machining system

  10. Reconfigurable materials handling system incorporating part tracking, routing and scheduling

    CSIR Research Space (South Africa)

    Naidu, P

    2006-07-01

    Full Text Available . Transmission range of 10m-30m indoors is common. RFID is a commonly available system which uses either low-cost passive Radio tags, or higher cost active tags, that an RF receiver can then read. An RFID system comprises of a reader, its associated antenna...]. 3. Proposed Tracking System The proposed tracking system consists of a two phases. In the first phase a passive radio frequency (RF) tag is read by a RF reader. In the second phase the information obtained from the reader is wirelessly...

  11. Contamination confinement system of irradiated materials handling laboratories

    International Nuclear Information System (INIS)

    Lobao, A. dos S.T.; Araujo, J.A. de; Camilo, R.L.

    1988-06-01

    A study to prevent radioctivity release in lab scale is presented. As a basis for the design all the limits established by the IAEA for ventilation systems were observed. An evaluation of the different parameters involved in the design have been made, resulting in the especification of the working areas, ducts and filtering systems in order to get the best conditions for the safe handling of irradiated materials. (author) [pt

  12. Robotic control architecture development for automated nuclear material handling systems

    International Nuclear Information System (INIS)

    Merrill, R.D.; Hurd, R.; Couture, S.; Wilhelmsen, K.

    1995-02-01

    Lawrence Livermore National Laboratory (LLNL) is engaged in developing automated systems for handling materials for mixed waste treatment, nuclear pyrochemical processing, and weapon components disassembly. In support of these application areas there is an extensive robotic development program. This paper will describe the portion of this effort at LLNL devoted to control system architecture development, and review two applications currently being implemented which incorporate these technologies

  13. Containment system of contamination in irradiated materials handling laboratories

    International Nuclear Information System (INIS)

    Lobao, A.S.T.; Araujo, J.A. de; Camilo, R.L.

    1988-01-01

    A study to prevent radiactivity release in lab scale is presented. As a basis for the design all the limits established by the IAEA for ventilation systems were observed. An evaluation of the different parameters involved in the design have been made, resulting in the specification of the working areas, ducts and filtering systems in order to get the best conditions for the safe handling of irradiated materials. (author) [pt

  14. Pilot material handling system for radiation processing of agricultural and medical products

    International Nuclear Information System (INIS)

    Sandha, R.S.; Nageswar Rao, J; Dwivedi, Jishnu; Petwal, V.C.; Soni, H.C.

    2005-01-01

    A 10 MeV, 10 kW electron LINAC based radiation processing facility is being constructed at Centre for Advanced Technology, Indore for radiation processing of various food products like potatoes, onion, spices, home pack items and medical sterilization. A pilot material handling system has been designed, manufactured, and installed at CAT to verify process parameters viz. conveying speed, dose uniformity, and to study the effect of packing shape and size for radiation processing of different product. This paper describes various features of pilot material handling system. (author)

  15. Generic Planning and Control of Automated Material Handling Systems: Practical Requirements Versus Existing Theroy.

    NARCIS (Netherlands)

    Haneyah, S.W.A.; Zijm, Willem H.M.; Schutten, Johannes M.J.; Schuur, Peter

    2011-01-01

    This paper discusses the problem of generic planning and control of Automated Material Handling Systems (AMHSs). The paper illustrates the relevance of this research direction, and then addresses three different market sectors where AMHSs are used. These market sectors are: baggage handling,

  16. Planning and control of automated material handling systems: The merge module

    NARCIS (Netherlands)

    Haneyah, S.W.A.; Hurink, Johann L.; Schutten, Johannes M.J.; Zijm, Willem H.M.; Schuur, Peter; Hu, Bo; Morasch, Karl; Pickl, Stefan; Siegle, Markus

    2011-01-01

    We address the field of internal logistics, embodied in Automated Material Handling Systems (AMHSs), which are complex installations employed in sectors such as Baggage Handling, Physical Distribution, and Parcel & Postal. We work on designing an integral planning and real-time control architecture,

  17. Generic planning and control of automated material handling systems : practical requirements versus existing theory

    NARCIS (Netherlands)

    Haneyah, S.W.A.; Schutten, Johannes M.J.; Schuur, Peter; Zijm, Willem H.M.

    2013-01-01

    This paper discusses the problem to design a generic planning and control architecture for utomated material handling systems (AMHSs). We illustrate the relevance of this research direction, and then address three different market sectors where AMHSs are used, i.e., baggage handling, distribution,

  18. Material handling systems for use in glovebox lines: A survey of Department of Energy facility experience

    International Nuclear Information System (INIS)

    Teese, G.D.; Randall, W.J.

    1992-01-01

    The Nuclear Weapons Complex Reconfiguration Study has recommended that a new manufacturing facility be constructed to replace the Rocky Flats Plant. In the new facility, use of an automated material handling system for movement of components would reduce both the cost and radiation exposure associated with production and maintenance operations. Contamination control would be improved between process steps through the use of airlocks and portals. Part damage associated with improper transport would be reduced, and accountability would be increased. In-process workpieces could be stored in a secure vault, awaiting a request for parts at a production station. However, all of these desirable features rely on the proper implementation of an automated material handling system. The Department of Energy Weapons Production Complex has experience with a variety of methods for transporting discrete parts in glovebox lines. The authors visited several sites to evaluate the existing technologies for their suitability for the application of plutonium manufacturing. Technologies reviewed were Linear motors, belt conveyors, roller conveyors, accumulating roller conveyors, pneumatic transport, and cart systems. The sites visited were The Idaho National Engineering laboratory, the Hanford Site, and the Rocky Flats Plant. Linear motors appear to be the most promising technology observed for the movement of discrete parts, and further investigation is recommended

  19. Manipulator and materials handling systems for reactor decommissioning -Cooperation between the university and the plant operator

    International Nuclear Information System (INIS)

    Schreck, G.; Bach, F. W.; Haferkamp, H.

    1995-01-01

    Nuclear reactor dismantling requires suitable handling systems for tools and disassembled components, as well as qualified and reliable disassembly and cutting techniques. From the angle of radiation protection, remote-controlled handling techniques and underwater techniques are the methods of choice, the latter particularly in continuation of plant operating conditions, and this all the more the more disassembly work proceeds towards the reactor core. With the experience accumulated for 20 years now by the Institut fuer Werkstoffkunde (materials science) of Hannover University by basic research and application-oriented development work in the field of thermal cutting technology, especially plasma arc cutting techniques, as well as development work in the field of remote-controlled materials handling systems, the institute is the cut-out partner for disassembly tasks in reactor decommissioning. (Orig./DG) [de

  20. Methodology on Investigating the Influences of Automated Material Handling System in Automotive Assembly Process

    Science.gov (United States)

    Saffar, Seha; Azni Jafar, Fairul; Jamaludin, Zamberi

    2016-02-01

    A case study was selected as a method to collect data in actual industry situation. The study aimed to assess the influences of automated material handling system in automotive industry by proposing a new design of integration system through simulation, and analyze the significant effect and influence of the system. The method approach tool will be CAD Software (Delmia & Quest). The process of preliminary data gathering in phase 1 will collect all data related from actual industry situation. It is expected to produce a guideline and limitation in designing a new integration system later. In phase 2, an idea or concept of design will be done by using 10 principles of design consideration for manufacturing. A full factorial design will be used as design of experiment in order to analyze the performance measured of the integration system with the current system in case study. From the result of the experiment, an ANOVA analysis will be done to study the performance measured. Thus, it is expected that influences can be seen from the improvement made in the system.

  1. Proposal for Construction/Demonstration/Implementation of A Material Handling System

    International Nuclear Information System (INIS)

    Jim Jnatt

    2001-01-01

    Vortec Corporation, the United States Enrichment Corporation (USEC) and DOE/Paducah propose to complete the technology demonstration and the implementation of the Material Handling System developed under Contract Number DE-AC21-92MC29120. The demonstration testing and operational implementation will be done at the Paducah Gaseous Diffusion Plant. The scope of work, schedule and cost for the activities are included in this proposal. A description of the facility to be constructed and tested is provided in Exhibit 1, attached. The USEC proposal for implementation at Paducah is presented in Exhibit 2, and the commitment letters from the site are included in Exhibit 3. Under our agreements with USEC, Bechtel Jacobs Corporation and DOE/Paducah, Vortec will be responsible for the construction of the demonstration facility as documented in the engineering design package submitted under Phase 4 of this contract on August 9, 2001. USEC will have responsibility for the demonstration testing and commercial implementation of the plant. The demonstration testing and initial commercial implementation of the technology will be achieved by means of a USEC work authorization task with the Bechtel Jacobs Corporation. The initial processing activities will include the processing of approximately 4,250 drums of LLW. Subsequent processing of LLW and TSCA/LLW will be done under a separate contract or work authorization task. To meet the schedule for commercial implementation, it is important that the execution of the Phase 4 project option for construction of the demonstration system be executed as soon as possible. The schedule we have presented herein assumes initiation of the construction phase by the end of September 2001. Vortec proposes to complete construction of the demonstration test system for an estimated cost of $3,254,422. This price is based on the design submitted to DOE/NETL under the Phase 4 engineering design deliverable (9 august 2001). The cost is subject to the

  2. DESIGN OF MANUAL MATERIAL HANDLING SYSTEM THROUGH COMPUTER AIDED ERGONOMICS: A CASE STUDY AT BDTSC TEXTILE FIRM

    Directory of Open Access Journals (Sweden)

    Amare Matebu

    2014-12-01

    Full Text Available Designing of lifting, pushing and pulling activities based on the physical and physiological capabilities of the operators is essential. The purpose of this study is to analyze manual material handling (MMH working posture of the operators using 3D Static Strength Prediction Program (3DSSPP software and to identify major areas causing long last injury of operators. The research has investigated the fit between the demands of tasks and the capabilities of operators. At the existing situations, the actual capabilities of operators have been computed with the help of 3DSSPP software and compared with NIOSH standards. Accordingly, operators' working posture is at an unacceptable position that exposes them for musculoskeletal disorders. Then, after the improvement of the design of MMH device (cart's roller, the result showed that the forces required by the operators to push and pull the sliver cans have been reduced from 931.77 Newton to 194.23 Newton. Furthermore, improvement of MMH cart's roller has reduced the awkward posture of operators and the risk of musculoskeletal disorders. The improved manual material handling design also saves about 1828.40 ETB per month for the company.

  3. CanDan 2, phase 2. Final report. [Fuel cell systems for back-up power and materials handling applications]; CanDan 2, fase 2. Slutrapport

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2012-11-01

    CanDan 2 Phase 2 is the second phase of a research and demonstration project for fuel cell backup power systems and fuel cell powered material handling equipment. In the Backup Power segment the fuel cell units have been developed, certified and delivered. A total of 32 fuel cell backup power systems have been delivered for EnergiMidt and in operation since early 2011. Following this project EnergiMidt has purchased another 31 systems in order to make a full transition from battery backup to fuel cell backup in their entire broadband network. In the material handling segment a 10 kW fuel cell system has been fully integrated in the fork lift truck, Dantruck 3000 Power Hydrogen. The result was a much more commercially mature product than expected from the beginning of the project. The result is a finished 2,5T fork lift truck which was presented at the CE-mat fair in April 2011. (LN)

  4. Ergonomic material-handling device

    Science.gov (United States)

    Barsnick, Lance E.; Zalk, David M.; Perry, Catherine M.; Biggs, Terry; Tageson, Robert E.

    2004-08-24

    A hand-held ergonomic material-handling device capable of moving heavy objects, such as large waste containers and other large objects requiring mechanical assistance. The ergonomic material-handling device can be used with neutral postures of the back, shoulders, wrists and knees, thereby reducing potential injury to the user. The device involves two key features: 1) gives the user the ability to adjust the height of the handles of the device to ergonomically fit the needs of the user's back, wrists and shoulders; and 2) has a rounded handlebar shape, as well as the size and configuration of the handles which keep the user's wrists in a neutral posture during manipulation of the device.

  5. 48 CFR 908.7112 - Materials handling equipment replacement standards.

    Science.gov (United States)

    2010-10-01

    ... equipment replacement standards. 908.7112 Section 908.7112 Federal Acquisition Regulations System DEPARTMENT... Special Items 908.7112 Materials handling equipment replacement standards. Materials handling equipment shall be purchased for replacement purposes in accordance with the standards in FPMR 41 CFR 101-25.405...

  6. Robotic Materials Handling in Space: Mechanical Design of the Robot Operated Materials Processing System HitchHiker Experiment

    Science.gov (United States)

    Voellmer, George

    1997-01-01

    The Goddard Space Flight Center has developed the Robot Operated Materials Processing System (ROMPS) that flew aboard STS-64 in September, 1994. The ROMPS robot transported pallets containing wafers of different materials from their storage racks to a furnace for thermal processing. A system of tapered guides and compliant springs was designed to deal with the potential misalignments. The robot and all the sample pallets were locked down for launch and landing. The design of the passive lockdown system, and the interplay between it and the alignment system are presented.

  7. Malzeme Taşıma Sistemi Alternatiflerinin Değerlendirilmesinde Bulanık-Promethee Yaklaşımı = Evaluating Material Handling System Alternatives Using Fuzzy-Promethee Approach

    Directory of Open Access Journals (Sweden)

    Bahadır GÜLSÜN

    2011-01-01

    Full Text Available Material handling system selection process has an important effect on productivity of manufacturing systems. Considering the importance of this process, it can be seen that there is a necessity to establish a systematic approach. In this study, to determine the best material handling system alternative, PROMETHEE approach is utilized. With the aim of taking into account the vagueness in the evaluation process and obtaining decision maker evaluations in an easier way with linguistic terms, fuzzy sets are utilized. To this end, a modified version of PROMETHEE is used. To foster the better understanding of the methodology, a real life application of a manufacturer is realized.

  8. 2010 Manufacturing Readiness Assessment Update to the 2008 Report for Fuel Cell Stacks and Systems for the Backup Power and Materials Handling Equipment Markets

    Energy Technology Data Exchange (ETDEWEB)

    Wheeler, D.; Ulsh, M.

    2012-08-01

    In 2008, the National Renewable Energy Laboratory (NREL), under contract to the US Department of Energy (DOE), conducted a manufacturing readiness assessment (MRA) of fuel cell systems and fuel cell stacks for back-up power and material handling applications (MHE). To facilitate the MRA, manufacturing readiness levels (MRL) were defined that were based on the Technology Readiness Levels previously established by the US Department of Energy (DOE). NREL assessed the extensive existing hierarchy of MRLs developed by Department of Defense (DoD) and other Federal entities, and developed a MRL scale adapted to the needs of the Fuel Cell Technologies Program (FCTP) and to the status of the fuel cell industry. The MRL ranking of a fuel cell manufacturing facility increases as the manufacturing capability transitions from laboratory prototype development through Low Rate Initial Production to Full Rate Production. DOE can use MRLs to address the economic and institutional risks associated with a ramp-up in polymer electrolyte membrane (PEM) fuel cell production. In 2010, NREL updated this assessment, including additional manufacturers, an assessment of market developments since the original report, and a comparison of MRLs between 2008 and 2010.

  9. An Improved Dispatching Method (a-HPDB for Automated Material Handling System with Active Rolling Belt for 450 mm Wafer Fabrication

    Directory of Open Access Journals (Sweden)

    Chia-Nan Wang

    2017-07-01

    Full Text Available The semiconductor industry is facing the transition from 300 mm to 450 mm wafer fabrication. Due to the increased size and weight, 450 mm wafers will pose unprecedented challenges on semiconductor wafer fabrication. To better handle and transport 450 mm wafers, an advanced Automated Material Handling System (AMHS is definitely required. Though conveyor-based AMHS is expected to be suitable for 450 mm wafer fabrication, still it faces two main problems, traffic-jam problem and lot-prioritization. To address the two problems, in this research we have proposed an improved dispatching method, termed Heuristic Preemptive Dispatching Method using Activated Roller Belt (a-HPDB. We have developed some effective rules for the a-HPDB based on Activated Roller Belt (ARB. In addition, we have conducted experiments to investigate its effectiveness. Compared with the HPDB and R-HPD, two dispatching rules proposed in previous studies, our experimental results showed the a-HPDB had a better performance in terms of average lot delivery time (ALDT. For hot lots and normal lots, the a-HPDB had advantages of 4.14% and 8.92% over the HPDB and advantages of 4.89% and 8.52% over R-HPD, respectively.

  10. HyLIFT-FLEX. ''Development and demonstration of flexible and scalable fuel cell power system for various material handling vehicles''. Final report

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2012-10-15

    The project has successfully developed and tested a new fuel cell system from H2 Logic in a tow tractor from MULAG. Based on the project results a positive decision has been taken on continuing commercialisation efforts. Next step will be a large scale demonstration of up to 100 units in a new project named HyLIFT-Europe that is expected to commence in early 2013, with support from the FCH-JU programme. Main efforts in the project have been the development of a new fuel cell system, named H2Drive from H2 Logic, and the integration and test in a standard battery powered COMET 3 towing tractor from MULAG. The system size is exactly the same as a standard battery box (DIN measures) and can be easily integrated into e.g. the MULAG vehicle or other electric powered material handling vehicles using the same battery size. Several R and D efforts on the fuel cell system have been conducted with the aim to reduce cost and improve efficiency, among others the following: 1) New air compressor sub-system and control - improving overall system efficiency with {approx}2,5%. 2) New simplified air-based compressor cooling sub-system. 3) New hydrogen compressor sub-system with improved efficiency and reduced cost. 4) New hydrogen inlet and outlet manifold sub-system - resulting in reduction of more than 50% of all sensor components in the fuel cell system. 5) New DC/DC converter with an average efficiency of 97% - a 3% improvement. 6) A new optimized hybrid system that meets the vehicle cycle requirements. In total the R and D efforts have improved the overall fuel cell system efficiency with 10% and helped to reduce costs with 33% compared to the previous generation. A first prototype of the developed H2Drive system has been constructed and integrated into the MULAG Towing Tractor. Only few modifications were made on the base vehicle, among others integration of cabin-heating, displays and motor control. Several internal tests were conducted at H2 Logic and MULAG before making a

  11. Regulatory process for material handling equipment

    International Nuclear Information System (INIS)

    Rajendran, S.; Agarwal, Kailash

    2017-01-01

    Atomic Energy (Factories) Rules (AEFR) 1996, Rule 35 states, 'Thorough inspection and load testing of a Crane shall be done by a Competent Person at least once every 12 months'. To adhere to this rule, BARC Safety Council constituted 'Material Handling Equipment Committee (MHEC)' under the aegis of Conventional Fire and Safety Review Committee (CFSRC) to carry out periodical inspection and certification of Material Handling Equipment (MHE), tools and tackles used in BARC Facilities at Trombay, Tarapur and Kalpakkam

  12. Safety requirements and feedback of commonly used material handling equipment

    International Nuclear Information System (INIS)

    Pathak, M.K.

    2009-01-01

    Different types of cranes, hoists, chain pulley blocks are the most commonly used material handling equipment in industry along with attachments like chains, wire rope slings, d-shackles, etc. These equipment are used at work for transferring loads from one place to another and attachments are used for anchoring, fixing or supporting the load. Selection of the correct equipment, identification of the equipment planning of material handling operation, examination/testing of the equipment, education and training of the persons engaged in operation of the material handling equipment can reduce the risks to safety of people in workplace. Different safety systems like boom angle indicator, overload tripping device, limit switches, etc. should be available in the cranes for their safe use. Safety requirement for safe operation of material handling equipment with emphasis on different cranes and attachments particularly wire rope slings and chain slings have been brought out in this paper. An attempt has also been made to bring out common nature of deficiencies observed during regulatory inspection carried out by AERB. (author)

  13. Design and Implementation of Company Tailored Automated Material Handling

    DEFF Research Database (Denmark)

    Langer, Gilad; Bilberg, Arne

    1996-01-01

    This article focuses on the problems of analysing automation of material handling systems in order to develop an efficient automated solution that is specifically tailored to the company. The research has resulted in development of new methods for evaluating factory automation from design...... to implementation. The goals of the research were to analyse and evaluate automation in order to obtain an advantageous combination of human and automated resources. The idea is to asses different solutions in a virtual environment, where experiments and analyses can be performed so that the company can justify...... for their application with computer aided information processing tools. The framework is named the "Automated Material Handling (AMH) Preference GuideLine". The research has been carried out in close co-operation with Danish and European industry, where implementations of automation can be referred to. It is our...

  14. Modeling Automated Guided Vehicle Systems in Material Handling = Otomatikleştirilmiş Rehberli Araç Sistemlerinin Transport Tekniğinde Modellemesi

    Directory of Open Access Journals (Sweden)

    Bülent SEZEN

    2003-06-01

    Full Text Available The study objectives are to 1 provide information regarding the use and benefits of Automated Guided Vehicle (AGV systems in manufacturing environments, and 2 review the literature related to design, modeling and simulation of AGV systems. We classify the tools utilized in design problems of AGV systems as analytical and simulation-based tools. Then, give examples of both categories from related literature.

  15. Trial operation of material protection, control, and accountability systems at two active nuclear material handling sites within the All-Russian Institute of Experimental Physics (VNIIEF)

    International Nuclear Information System (INIS)

    Skripka, G.; Vatulin, V.; Yuferev, V.

    1997-01-01

    This paper discusses Russian Federal Nuclear Center (RFNC)-VNIIEF activities in the area of nuclear material protection, control, and accounting (MPC and A) procedures enhancement. The goal of such activities is the development of an automated systems for MPC and A at two of the active VNIIEF research sites: a research (reactor) site and a nuclear material production facility. The activities for MPC and A system enhancement at both sites are performed in the framework of a VNIIEF-Los Alamos National Laboratory contract with participation from Sandia National Laboratories, Lawrence Livermore National Laboratory, Brookhaven National Laboratory, Oak Ridge National Laboratory, Pacific Northwest National Laboratory, and PANTEX Plant in accordance with Russian programs supported by MinAtom. The American specialists took part in searching for possible improvement of technical solutions, ordering equipment, and delivering and testing the equipment that was provided by the Americans

  16. Preconceptual design of a Long-Pulse Spallation Source (LPSS) at the LANSCE Facility: Target system, facility, and material handling considerations

    International Nuclear Information System (INIS)

    Sommer, W.F.

    1995-12-01

    This report provides a summary of a preconceptual design study for the proposed Long-Pulse Spallation. Source (LPSS) at the Los Alamos Neutron Science Center (LANSCE). The LPSS will use a 0.8-MW proton beam to produce neutrons from a tungsten target. This study focuses on the design of the target station and changes to the existing building that would be made to accommodate the LPSS. The LPSS will provide fifteen flight paths to neutron scattering instruments. In addition, options for generating ultracold neutrons, pions, and muons will be available. Flight-energy, forward-scattered neutrons on the downstream side of the target will also be available for autoradiography studies. A Target Test Bed (TTB) is also proposed for full-beam tests of component materials and advanced spallation neutron sources. The design allows for separation of the experiment hall from the beam line, target, and flight paths. The target and moderator systems and the systems/components to be tested in the TTB will be emplaced and removed separately by remotely operated, shielded equipment. Irradiated materials will be transported to a hot cell adjacent to the target chamber for testing by remotely operated instruments. These tests will provide information about how materials properties are affected by proton and neutron beams

  17. Materials Handling. Module SH-01. Safety and Health.

    Science.gov (United States)

    Center for Occupational Research and Development, Inc., Waco, TX.

    This student module on materials handling is one of 50 modules concerned with job safety and health. It presents the procedures for safe materials handling. Discussed are manual handling methods (lifting and carrying by hand) and mechanical lifting (lifting by powered trucks, cranes or conveyors). Following the introduction, 15 objectives (each…

  18. Design and operation of a remotely operated plutonium waste size reduction and material handling process

    International Nuclear Information System (INIS)

    Stewart, J.A. III; Charlesworth, D.L.

    1986-01-01

    Noncombustible 238 Pu and 239 Pu waste is generated as a result of normal operation and decommissioning activity at the Savannah River Plant, and is being retrievably stored there. As part of the long-term plant to process the stored waste and current waste for permanent disposal, a remote size reduction and material handling process is being cold-tested at Savannah River Laboratory. The process consists of a large, low-speed shredder and material handling system, a remote worktable, a bagless transfer system, and a robotically controlled manipulator. Initial testing of the shredder and material handling system and a cycle test of the bagless transfer system has been completed. Fabrication and acceptance testing of the Telerobat, a robotically controlled manipulator has been completed. Testing is scheduled to begin in 3/86. Design features maximizing the ability to remotely maintain the equipment were incorporated. Complete cold-testing of the equipment is scheduled to be completed in 1987

  19. Experience in testing and inspection and maintenance of material handling equipments

    International Nuclear Information System (INIS)

    Sharma, M.L.

    2009-01-01

    All the Industries, Power Projects/Stations, Organizations engaged in the field of process of manufacturing, power generation, transportation, design, layout, manufacturing, and supply have to utilize material handling equipment, machinery tools tackles, lifting gears for performing their tasks/activities. The major role of the material handling equipments play an important role and a component of 40% of the total activities of the system/process to achieve targeted output with the reliability and quality is performed by material handling equipment and machineries. The material handling equipment shall have to be chosen/selected to suit the process requirement at times to be specifically designed inspected and tested to meet the specific requirement. These equipment/machineries/lifting gears have to undergo for the periodical inspection and testing to qualify for further use in a specified period. All those equipment and machinery to be used for material handling if not found satisfactory during inspection and testing or otherwise also shall be dismantled/stripped to the extent of inspection requirement of the components/sub components and maintenance repair shall have to be done to make them worthy for reuse after testing and inspection duly witnessed by competent authority

  20. Fleet Sizing of Automated Material Handling Using Simulation Approach

    Science.gov (United States)

    Wibisono, Radinal; Ai, The Jin; Ratna Yuniartha, Deny

    2018-03-01

    Automated material handling tends to be chosen rather than using human power in material handling activity for production floor in manufacturing company. One critical issue in implementing automated material handling is designing phase to ensure that material handling activity more efficient in term of cost spending. Fleet sizing become one of the topic in designing phase. In this research, simulation approach is being used to solve fleet sizing problem in flow shop production to ensure optimum situation. Optimum situation in this research means minimum flow time and maximum capacity in production floor. Simulation approach is being used because flow shop can be modelled into queuing network and inter-arrival time is not following exponential distribution. Therefore, contribution of this research is solving fleet sizing problem with multi objectives in flow shop production using simulation approach with ARENA Software

  1. Decision method for optimal selection of warehouse material handling strategies by production companies

    Science.gov (United States)

    Dobos, P.; Tamás, P.; Illés, B.

    2016-11-01

    Adequate establishment and operation of warehouse logistics determines the companies’ competitiveness significantly because it effects greatly the quality and the selling price of the goods that the production companies produce. In order to implement and manage an adequate warehouse system, adequate warehouse position, stock management model, warehouse technology, motivated work force committed to process improvement and material handling strategy are necessary. In practical life, companies have paid small attantion to select the warehouse strategy properly. Although it has a major influence on the production in the case of material warehouse and on smooth costumer service in the case of finished goods warehouse because this can happen with a huge loss in material handling. Due to the dynamically changing production structure, frequent reorganization of warehouse activities is needed, on what the majority of the companies react basically with no reactions. This work presents a simulation test system frames for eligible warehouse material handling strategy selection and also the decision method for selection.

  2. ASRS application in radioactive material handling

    International Nuclear Information System (INIS)

    Shalina Sheik Muhamad and Rosli Darmawan

    2007-01-01

    ASRS is a computer controlled methods for automatically depositing and retrieving loads from defined storage locations. It consists of several major components which are the Controls System, Storage Structure (Racks), Storage/Retrieval (S/R) Machines, Fire Protection Systems, Pickup and Delivery (P and D) Stations, Storage Modules (Pallets, Baskets, Containers), Aisle Hardware, Aisle Transfer Cars, Conveyors and other transportation equipment. This paper aims to provide an overview of the potential application of the ASRS in Nuclear Malaysia. The need to use the system, operating principle and potential application will be discussed. (Author)

  3. Radioactive material handling for radiopharmaceutical production

    International Nuclear Information System (INIS)

    Anwar Abd Rahman; Rosli Darmawan; Mohd Khairi Mohd Said; Mohd Arif Hamzah; Mohd Fadil Ismail; Mohd Nor Atan; Mohd Azam Safawi Omar; Zulkifli Hashim; Wan Anuar Wan Awang

    2005-01-01

    Construction of clean room at Block 21 had changed the flow of radioactive material Moly-99 into the hotcell. The existing flow which use the transport cask cannot be used in order to prevent the clean room from contamination. Therefore, the new technique which consist of robotic, pneumatic and transfer box system had been introduced to transfer the radioactive source into the hotcell without going through the clean room.This technique that has been introduced provides safety where the radiation workers control the transfer process by using remote system. (Author)

  4. Controlling fugitive dust emissions in material handling operations

    Energy Technology Data Exchange (ETDEWEB)

    Tooker, G E

    1992-05-01

    The primary mechanism of fugitive dust generation in bulk material handling transfer operations is by dispersion of dust in turbulent air induced to flow with falling or projected material streams. This paper returns to basic theories of particle dynamics and fluid mechanics to quantify the dust generating mechanism by rational analysis. Calculations involving fluid mechanisms are made easier by the availability of the personal computer and the many math manipulating programs. Rational analysis is much more cost effective when estimating collection air volumes to control fugitive emissions; especially in enclosed material handling transfers transporting large volumes of dusty material. Example calculations, using a typical enclosed conveyor-to-conveyor transfer operation are presented to illustrate and highlight the key parameters that determine the magnitude of induced air flow that must be controlled. The methods presented in this paper for estimating collection air volumes apply only enclosed material handling transfers, exhausted to a dust collector. Since some assistance to the control of dust emissions must be given by the material handling transfer chute design, a discussion of good transfer chute design practice is presented. 4 refs., 2 figs., 2 tabs.

  5. Demonstration of a remotely operated TRU waste size-reduction and material handling process

    International Nuclear Information System (INIS)

    Stewart, J.A. III; Schuler, T.F.; Ward, C.R.

    1986-01-01

    Noncombustible Pu-238 and Pu-239 waste is generated as a result of normal operation and decommissioning activity at the Savannah River Plant and is being retrievably stored at the site. As part of the long-term plan to process the stored waste and current waste for permanent disposal, a remote size-reduction and material handling process is being tested at Savannah River Laboratory to provide design support for the plant TRU Waste Facility scheduled to be completed in 1993. The process consists of a large, low-speed shredder and material handling system, a remote worktable, a bagless transfer system, and a robotically controlled manipulator, or Telerobot. Initial testing of the shredder and material handling system and a cycle test of the bagless transfer system were completed. Initial Telerobot run-in and system evaluation was completed. User software was evaluated and modified to support complete menu-driven operation. Telerobot prototype size-reduction tooling was designed and successfully tested. Complete nonradioactive testing of the equipment is scheduled to be completed in 1987

  6. REMOTE MATERIAL HANDLING IN THE YUCCA MOUNTAIN WASTE PACKAGE CLOSURE CELL AND SUPPORT AREA GLOVEBOX

    International Nuclear Information System (INIS)

    K.M. Croft; S.M. Allen; M.W. Borland

    2005-01-01

    The Yucca Mountain Waste Package Closure System (WPCS) cells provide for shielding of highly radioactive materials contained in unsealed waste packages. The purpose of the cells is to provide safe environments for package handling and sealing operations. Once sealed, the packages are placed in the Yucca Mountain Repository. Closure of a typical waste package involves a number of remote operations. Those involved typically include the placement of matched lids onto the waste package. The lids are then individually sealed to the waste package by welding. Currently, the waste package includes three lids. One lid is placed before movement of the waste package to the closure cell; the final two are placed inside the closure cell, where they are welded to the waste package. These and other important operations require considerable remote material handling within the cell environment. This paper discusses the remote material handling equipment, designs, functions, operations, and maintenance, relative to waste package closure

  7. Manual materials handling: the cause of over-exertion injury and illness in industry.

    Science.gov (United States)

    Chaffin, D B

    1979-01-01

    It is reported from various sources that overexertion due to lifting, pushing, pulling, and carrying objects accounts for about 27 percent of all compensable industrial injury and illness in the United States. Resulting strain/sprain injuries account for over 50 percent of workmen's compensation clams in many industries. Almont two-thirds of these involve back pain, with reported compensation and medical payments totaling well over one billion dollars annually in the U.S. An estimated 300,000 plus workers will be affected each year, 5 to 10 percent of whom will have a permanent disability and often will be unemployable. This paper attempts to describe four basic approaches used to study this occupational health problem. In so doing, a concerted effort is made to identifiy the gaps in knowledge which need to be more fully researched. The approaches utilized to understand and control the hazards of manual materials handling are: 1) epidemiological studies of job and worker attributes to identify those that individually and in combination cause musculoskeletal incidents, 2) psychophysical studies to ascertain the volitional tolerance of workers to the stress mitigated by manual materials-handling activities, 3) biomechanical studies of the musculoskeletal system during common exertions required in manual materials-handling activities, and 4) physiological studies of the strain imposed on the cardiovascular system during repeated load-handling activities. The state of knowledge from each of these approaches is summarized briefly, and a case is made that much research is still needed to substantiate the necessary controls to lessen the economic burden and human suffering associated with manual materials-handling acts in industry.

  8. Remote automated material handling of radioactive waste containers

    International Nuclear Information System (INIS)

    Greager, T.M.

    1994-09-01

    To enhance personnel safety, improve productivity, and reduce costs, the design team incorporated a remote, automated stacker/retriever, automatic inspection, and automated guidance vehicle for material handling at the Enhanced Radioactive and Mixed Waste Storage Facility - Phase V (Phase V Storage Facility) on the Hanford Site in south-central Washington State. The Phase V Storage Facility, scheduled to begin operation in mid-1997, is the first low-cost facility of its kind to use this technology for handling drums. Since 1970, the Hanford Site's suspect transuranic (TRU) wastes and, more recently, mixed wastes (both low-level and TRU) have been accumulating in storage awaiting treatment and disposal. Currently, the Hanford Site is only capable of onsite disposal of radioactive low-level waste (LLW). Nonradioactive hazardous wastes must be shipped off site for treatment. The Waste Receiving and Processing (WRAP) facilities will provide the primary treatment capability for solid-waste storage at the Hanford Site. The Phase V Storage Facility, which accommodates 27,000 drum equivalents of contact-handled waste, will provide the following critical functions for the efficient operation of the WRAP facilities: (1) Shipping/Receiving; (2) Head Space Gas Sampling; (3) Inventory Control; (4) Storage; (5) Automated/Manual Material Handling

  9. Investigation into slipping and falling accidents and materials handling in the South African mining industry.

    CSIR Research Space (South Africa)

    Schutte, PC

    2003-03-01

    Full Text Available The objective of this study was to analyze information on slipping and falling accidents and materials handling activities in the South African mining industry. Accident data pertaining to slipping, falling and materials handling accidents...

  10. Method of preventing contaminations in radioactive material handling facilities

    International Nuclear Information System (INIS)

    Inoue, Shunji.

    1986-01-01

    Purpose: To prevent the contamination on the floor surface of working places by laying polyvinyl butyral sheets over the floor surface, replacing when the sheets are contaminated, followed by burning. Method: Polyvinyl butyral sheets comprising 50 - 70 mol% of butyral component are laid in a radioactive material handling facility, radioactive materials are handled on the polyvinyl butyral sheets and the sheets are replaced when contaminated. The polyvinyl butyral sheets used contain 62 - 68 mol% of butyral component and has 0.03 - 0.2 mm thickness. The contaminated sheets are subjected to burning processing. This can surely collect radioactive materials and the sheets have favorable burnability, releasing no corrosive or deleterious gases. In addition, they are inexpensive and give no hindrance to the workers walking. (Takahashi, M.)

  11. Material handling for the Los Alamos National Laboratory Nuclear Storage Facility

    International Nuclear Information System (INIS)

    Pittman, P.; Roybal, J.; Durrer, R.; Gordon, D.

    1999-01-01

    This paper will present the design and application of material handling and automation systems currently being developed for the Los Alamos National Laboratory (LANL) Nuclear Material Storage Facility (NMSF) renovation project. The NMSF is a long-term storage facility for nuclear material in various forms. The material is stored within tubes in a rack called a basket. The material handling equipment range from simple lift assist devices to more sophisticated fully automated robots, and are split into three basic systems: a Vault Automation System, an NDA automation System, and a Drum handling System. The Vault Automation system provides a mechanism to handle a basket of material cans and to load/unload storage tubes within the material vault. In addition, another robot is provided to load/unload material cans within the baskets. The NDA Automation System provides a mechanism to move material within the small canister NDA laboratory and to load/unload the NDA instruments. The Drum Handling System consists of a series of off the shelf components used to assist in lifting heavy objects such as pallets of material or drums and barrels

  12. Energy-saving methodology for material handling applications

    Energy Technology Data Exchange (ETDEWEB)

    Makris, P.A.; Makri, A.P.; Provatidis, C.G. [National Technical University of Athens, School of Mechanical Engineering, Mechanical Design and Control Systems Division, 9 Iroon Polytechniou Street, Zografou Campus, GR-15773 Athens (Greece)

    2006-10-15

    This paper presents an energy saving approach to the problem of order picking in warehousing environment, which is directly related to the well-known Traveling Salesman Problem (TSP). While the available heuristic algorithms for the order-picking problem search for the route that minimizes the travel time, here the problem is addressed from the energy saving point of view. In a few words, the least energy-consuming route is identified in order to quantify the trade off in time and energy between the fastest route and the most energy economic one. Keeping in mind that often energy is as important as time, especially during a low-demand period, the current paper sheds some light into a two dimensional way of addressing the warehouse material handling problem, which saves time as well as energy. A very interesting finding is that a relatively small loss of service time in many cases may lead to a significant decrease of consumed energy without any additional cost. (author)

  13. Assessing materials handling and storage capacities in port terminals

    Science.gov (United States)

    Dinu, O.; Roşca, E.; Popa, M.; Roşca, M. A.; Rusca, A.

    2017-08-01

    Terminals constitute the factual interface between different modes and, as a result, buffer stocks are unavoidable whenever transport flows with different discontinuities meet. This is the reason why assessing materials handling and storage capacities is an important issue in the course of attempting to increase operative planning of logistic processes in terminals. Proposed paper starts with a brief review of the compatibilities between different sorts of materials and corresponding transport modes and after, a literature overview of the studies related to ports terminals and their specialization is made. As a methodology, discrete event simulation stands as a feasible technique for assessing handling and storage capacities at the terminal, taking into consideration the multi-flows interaction and the non-uniform arrivals of vessels and inland vehicles. In this context, a simulation model, that integrates the activities of an inland water terminal and describes the essential interactions between the subsystems which influence the terminal capacity, is developed. Different scenarios are simulated for diverse sorts of materials, leading to bottlenecks identification, performance indicators such as average storage occupancy rate, average dwell or transit times estimations, and their evolution is analysed in order to improve the transfer operations in the logistic process

  14. Evaluation of the Total Cost of Ownership of Fuel Cell-Powered Material Handling Equipment

    Energy Technology Data Exchange (ETDEWEB)

    Ramsden, T.

    2013-04-01

    This report discusses an analysis of the total cost of ownership of fuel cell-powered and traditional battery-powered material handling equipment (MHE, or more typically 'forklifts'). A number of fuel cell MHE deployments have received funding support from the federal government. Using data from these government co-funded deployments, DOE's National Renewable Energy Laboratory (NREL) has been evaluating the performance of fuel cells in material handling applications. NREL has assessed the total cost of ownership of fuel cell MHE and compared it to the cost of ownership of traditional battery-powered MHE. As part of its cost of ownership assessment, NREL looked at a range of costs associated with MHE operation, including the capital costs of battery and fuel cell systems, the cost of supporting infrastructure, maintenance costs, warehouse space costs, and labor costs. Considering all these costs, NREL found that fuel cell MHE can have a lower overall cost of ownership than comparable battery-powered MHE.

  15. 77 FR 23117 - Rigging Equipment for Material Handling Construction Standard; Correction and Technical Amendment

    Science.gov (United States)

    2012-04-18

    ... Equipment for Material Handling Construction Standard; Correction and Technical Amendment AGENCY... AND HEALTH REGULATIONS FOR CONSTRUCTION Subpart H--Materials Handling, Storage, Use, and Disposal 0 1... amendment. SUMMARY: OSHA is correcting its sling standard for construction titled ``Rigging Equipment for...

  16. Domestic round robin exercise on analysis of uranium for nuclear material handling facilities in Japan

    International Nuclear Information System (INIS)

    Kato, Yoshiyasu; Nagai, Kohta; Handa, Takamitsu; Inoue, Shin-ichi; Sato, Yoshihiro

    2016-01-01

    Interlaboratory comparison programme as well as internal quality control system is an effective tool for an analytical laboratory responsible to nuclear material accountancy of a nuclear facility to maintain and enhance its capability for analysis. However, it is a burden on nuclear material handling facilities in Japan to attend interlaboratory comparison programme run by overseas institutions because of high costs and complicated procedure for importing nuclear materials, and therefore facilities which can participate in such international programme would be limited. Nuclear Material Control Center has hence started and organised an annual domestic round robin exercise on analysis of uranium standard materials, funded by the Japan Safeguards Office of the Nuclear Regulation Authority, since 2008 to enhance analytical capability of Japanese Facilities. The outline of the round robin exercise will be given and the results of uranium isotopic and concentration analysis reported by participant facilities from 2008 to 2015 will be summarised in the presentation. (author)

  17. Retrospective search on biomass harvesting techniques including materials handling and storage

    Energy Technology Data Exchange (ETDEWEB)

    1985-10-01

    This literature search covers the period 1977 to date. The harvesting, materials handling and storage of the following materials: wood; crops and crop residues; peat; sugar cane; reeds, grasses and fers; algae and jojoba shrubs are covered.

  18. Mobile materials handling platform interface architecture for mass production environments

    CSIR Research Space (South Africa)

    Walker, A

    2008-01-01

    Full Text Available Industrial manufacturing systems achieve production stability due to near constant production processes e.g. mass production. Passive methods such as production flow analysis can produce plant layouts which optimise material flow within...

  19. Regulatory inspection practices for industrial safety (electrical, mechanical, material handling and conventional aspects)

    International Nuclear Information System (INIS)

    Agarwal, K.

    2017-01-01

    Regulatory Inspection (RI) of BARC facilities and projects are carried out under the guidance of BARC Safety Council (BSC) Secretariat. Basically facilities and projects have been divided into two board categories viz. radiological facilities and non-radiological facilities. The Rls of radiological facilities should be carried out under OPSRC and of non-radiological facilities under CFSRC. Periodicity of inspection shall be at least once in a year. The RI of projects is carried out under concerned DSRC. RI practices with industrial safety which includes electrical, mechanical, material handling and conventional aspect for these facilities starts with check lists. The inspection areas are prepared in the form of checklists which includes availability of approved documents, compliance status of previous RIT and various safety committee's recommendations, radiological status of facilities, prompt reporting of safety related unusual occurrences, major incident, site visit for verification of actual status of system/plant. The practices for inspection in the area of electrical safety shall include checking of maintenance procedure for all critical class IV system equipment's such as HT panel, LT panel, transformer and motors. Load testing of Class III system such as D.G. set etc. shall be carried out as technical specification surveillance schedule. Status of aviation lights, number of qualified staff, availability of qualified staff etc. shall be form of inspection

  20. MICRO-MATERIAL HANDLING EMPLOYING E-BEAM GENERATED TOPOGRAPHIES OF COPPER AND ALUMINIUM

    Directory of Open Access Journals (Sweden)

    S. Matope

    2012-01-01

    Full Text Available

    ENGLISH ABSTRACT: This paper focuses on the employment of copper and aluminium in a micro-material handling system actuated by Van der Waals forces. Electron beam (e-beam evaporator deposited both materials on a silicon substrate at a rate of 0.6-1.2 Angstroms/second, vacuum pressure between 2x10-6 and 3x10-6mbar, and at a current less than 10mA. A Veeco NanoMan V Atomic Force Microscope with Nanoscope version 7.3 software was used to analyse the root mean square (rms surface roughnesses of the generated topographies. Rumpf-Rabinovich’s rms formula was used to determine the Van der Waals forces exerted by the surfaces. It was synthesised that an e-beam deposition of 7 minutes’ duration on both materials produced an optimum micro-material handling solution, with copper suitable for the pick-up position and aluminium for the placement position.

    AFRIKAANSE OPSOMMING: Die fokus van die artikel is op die gebruik van koper en aluminium in ‘n mikromateriaalhanteringstelsel, aangedryf deur Van der Waalskragte. ‘n Elektronstraal-verdamper plaas albei materiale op ‘n silikonbasis teen ‘n tempo van 0.6-1.2 Angstrom/sekonde, vakuumdruk tussen 2x10-6 en 3x10-6mbar, en teen ‘n stroom van minder as 10mA. ‘n Veeco NanoMan V Atomic Force mikroskoop, met Nanoscope 7.3 program-matuur is gebruik om die wortel-gemiddelde-kwadraat (wgk oppervlak ruheid van die gegenereerde topografieë te analiseer. Rumpf-Rabinovich se wgk-formule is gebruik om die Van der Waalskrage wat deur die oppervlaktes uitgeoefen word te bepaal. Dit is vasgestel dat ‘n elektronstraalafsetting van 7 minute op albei materiale die optimale materiaalhanteringoplossing bied, met koper geskik vir die optelposisie en aluminium vir die plasingsposisie.

  1. PEMBENTUKAN SEL-SEL MESIN UNTUK MENDAPATKAN PENGURANGAN JARAK DAN BIAYA MATERIAL HANDLING DENGAN METODE HEURISTIK DI PT. BENGKEL COKRO BERSAUDARA

    Directory of Open Access Journals (Sweden)

    Bambang Purwanggono

    2012-02-01

    Full Text Available Tata letak pabrik dapat didefinisikan sebagai tata cara pengaturan fasilitas-fasilitas pabrik dengan memanfaatkan luas seoptimal mungkin guna menunjang kelancaran proses produksi. Tata letak fasilitas pada PT. Cokro Bersaudara diatur berdasarkan process layout dimana segala jenis mesin / fasilitas produksi lainnya yang memiliki tipe atau jenis yang sama ditempatkan dalam satu tempat. Dengan layout seperti itu perusahaan memperoleh keuntungan berupa fleksibilitas dalam memproduksi produk yang memiliki tingkat variasi yang tinggi, namun sebagai akibatnya perusahaan menghadapi permasalahan berupa tingginya kebutuhan material handling. Cellular Manufacturing System adalah aplikasi dari Group Technology yang merupakan metode pengaturan fasilitas-fasilitas produksi yang dibutuhkan untuk memproses suatu part family tertentu kedalam sel manufaktur. Dengan menerapkan Cellular Manufacturing System dapat diketahui pengurangan jarak antar mesin dan biaya material handling. Berdasarkan pengolahan data menggunakan algoritma heuristik yaitu Bond Energy Algorithm (BEA, Rank Order Clustering (ROC, dan Rank Order Clustering 2 (ROC 2 disimpulkan bahwa metode terpilih adalah metode BEA, dengan mengelompokkan 6 mesin (M dan 6 komponen (P kedalam 2 sel manufaktur, dimana sel 1 (M4, M6, M1, M2, P2, P5, P6, P1 dan sel 2 (M4, M6, M1, M3, M5, P3, P4. Dengan perubahan layout ini didapatkan pengurangan total jarak material handling sebesar 428,06 meter dan pengurangan biaya material handling sebesar Rp. 2.111.316,058 / bulan Kata Kunci : Cellular Manufacturing System, Algoritma Heuristik, Gorup Technology

  2. Demonstration of remotely operated TRU waste size reduction and material handling equipment

    International Nuclear Information System (INIS)

    Looper, M.G.; Charlesworth, D.L.

    1988-01-01

    The Savannah River Laboratory (SRL) is developing remote size reduction and material handling equipment to prepare 238 Pu contaminated waste for permanent disposal at the Waste Isolation Pilot Plant (WIPP) in New Mexico. The waste is generated at the Savannah River Plant (SRP) from normal operation and decommissioning activity and is retrievably stored onsite. A Transuranic Waste Facility for preparing, size-reducing, and packaging this waste for disposal is scheduled for completion in 1995. A cold test facility for demonstrating the size reduction and material handling equipment was built, and testing began in January 1987. 9 figs., 1 tab

  3. 29 CFR 1926.1000 - Rollover protective structures (ROPS) for material handling equipment.

    Science.gov (United States)

    2010-07-01

    ... 29 Labor 8 2010-07-01 2010-07-01 false Rollover protective structures (ROPS) for material handling equipment. 1926.1000 Section 1926.1000 Labor Regulations Relating to Labor (Continued) OCCUPATIONAL SAFETY... CONSTRUCTION Rollover Protective Structures; Overhead Protection § 1926.1000 Rollover protective structures...

  4. The cost analysis of material handling in Chinese traditional praying paper production plant

    Science.gov (United States)

    Nasution, H.; Budiman, I.; Salim, A.

    2018-02-01

    Chinese traditional praying paper industry is an industry which produced Chinese traditional religion praying paper. This kind of industry is rarely examined since it was only in Small and Medium Enterprise (SME’s- form). This industry produced various kinds of Chinese traditional paper products. The purpose of this research is to increase the amount of production, reduce waiting time and moving time, and reduce material handling cost. The research was conducted at prime production activities, consists of: calculate the capacity of the material handler, the frequency of movement, cost of material handling, and total cost of material handling. This displacement condition leads to an ineffective and inefficient production process. The alternative was developed using production judgment and aisle standard. Based on the observation results, it is possible to reduce displacement in the production. Using alternative which by-passed displacement from a rolled paper in the temporary warehouse to cutting and printing workstation, it can reduce material handling cost from 2.26 million rupiahs to 2.00 million rupiahs only for each batch of production. This result leads to increasing of production quantity, reducing waiting and moving time about 10% from the current condition.

  5. A conceptual framework for outsourcing of materials handling activities in automotive : differentiation and implementation

    NARCIS (Netherlands)

    Klingenberg, W.; Boksma, J. D.

    2010-01-01

    This article discusses the outsourcing of materials handling activities and investigates different options for its implementation. The article uses descriptive case studies found in literature from the Western European automotive industry to map out differences in current practice and to evaluate

  6. 78 FR 70326 - Rigging Equipment for Material Handling; Extension of the Office of Management and Budget's (OMB...

    Science.gov (United States)

    2013-11-25

    ...] Rigging Equipment for Material Handling; Extension of the Office of Management and Budget's (OMB) Approval... on Rigging Equipment for Material Handling (29 CFR 1926.251). These paragraphs require affixing... automated or other technological information collection and transmission techniques. III. Proposed Actions...

  7. Transportation flow analysis in a centralised supply chain at Toyota Material Handling Europe

    OpenAIRE

    Gustavsson, Styrbjörn; Öberg, Mikael

    2010-01-01

    This report is the result of a Master Thesis written at Toyota Material Handling Europe (TMHE), with the purpose to map the existing spare part supply chain structure and to analyse future suggested supply chain structures with a focus on transportation flows. TMHE is one of the world’s largest producing forklift distributers with large market shares throughout Europe. Besides the main activity of forklifts the aftermarket of distributing spare parts is a major business. With a current decent...

  8. Optimal Scheduling of Material Handling Devices in a PCB Production Line: Problem Formulation and a Polynomial Algorithm

    Directory of Open Access Journals (Sweden)

    Ada Che

    2008-01-01

    Full Text Available Modern automated production lines usually use one or multiple computer-controlled robots or hoists for material handling between workstations. A typical application of such lines is an automated electroplating line for processing printed circuit boards (PCBs. In these systems, cyclic production policy is widely used due to large lot size and simplicity of implementation. This paper addresses cyclic scheduling of a multihoist electroplating line with constant processing times. The objective is to minimize the cycle time, or equivalently to maximize the production throughput, for a given number of hoists. We propose a mathematical model and a polynomial algorithm for this scheduling problem. Computational results on randomly generated instances are reported.

  9. A national cross-sectional study in the Danish wood and furniture industry on working postures and manual materials handling.

    Science.gov (United States)

    Christensen, H; Pedersen, M B; Sjøgaard, G

    1995-04-01

    Musculoskeletal disorders constitute a major problem in the wood and furniture industry and identification of risk factors is needed urgently. Therefore, exposures to different work tasks and variation in the job were recorded based on an observation survey in combination with an interview among 281 employees working in wood working and painting departments. A questionnaire survey confirmed high frequencies of symptoms from the musculoskeletal system: The one-year prevalence of symptoms from the low back was 42% and symptoms from the neck/shoulder was 40%. The exposure was evaluated based on: (1) classification of work tasks, (2) work cycle time, (3) manual materials handling, (4) working postures, and (5) variation in the job. Among the employees 47% performed feeding or clearing of machines, 35% performed wood working or painting materials, and 18% performed various other operations. Among the employees 20% had no variation in their job while 44% had little variation. Manual materials handling of 375 different burdens was observed, which most often occurred during feeding or clearing of machines. The weight of burdens lifted was 0.5-87.0 kg, where 2% had a weight of more than 50 kg. Among the lifting conditions 30% were evaluated as implying a risk of injury. An additional risk factor was the high total tonnage lifted per day, which was estimated to range from 132 kg to 58,800 kg. Working postures implied a risk of injury due to prolonged forward and lateral flexions of the neck, which was seen most frequently during wood working or painting materials. These data substantiate the finding that work tasks mainly during feeding or clearing of machines imply a risk of injury to the low back and a risk of injury to the neck and shoulder area mainly during wood working or painting materials. Optimal strategies for job redesign may be worked out by using these data in order to prevent occupational musculoskeletal disorders.

  10. [Manual material handling risk assessment and ergonomic improvement in foodstuff retailing company].

    Science.gov (United States)

    Maso, S; Maccà, I; Simonetti, A; Scopa, P; Paruzzolo, P; Bonacci, A; Murgolo, I; Bartolucci, G B

    2011-01-01

    The aim of this study is to assess and reduce the risk due to manual material handling in a company involved in the foodstuff retailing. The risk assessment was performed by NIOSH Variable Lifing Index in 13 different occupational conditions. As result the risk was present in any case, with VLI values ranging from 2.12 to 2.81. A good risk reduction has been accomplished correcting properly the most important multiplier involved in the computation of the revised NIOSH Lifting equation (lifting frequency and weight of heavier products). Even if the performed risk reduction has been significant, the residual risk is still higher than the level of acceptability.

  11. Application of a layout/material handling design method to a furnace area in a 300 mm wafer fab

    NARCIS (Netherlands)

    Hesen, P.M.C.; Renders, P.J.J.; Rooda, J.E.

    2001-01-01

    For many years, material handling within the semiconductor industry has become increasingly important. With the introduction of 300 mm wafer production, ergonomics and product safety become more critical. Therefore, the manufacturers of semiconductor wafer fabs are considering the automation of

  12. Minimizing the carbon footprint of material handling equipment: Comparison of electric and LPG forklifts

    Energy Technology Data Exchange (ETDEWEB)

    Facchini, F.; Mummolo, G.; Mossa, G.; Digiesi, S.; Boenzi, F.; Verriello, R.

    2016-07-01

    Purpose: The aim of this study is to identify the best Material Handling Equipment (MHE) to minimize the carbon footprint of inbound logistic activities, based on the type of the warehouse (layout, facilities and order-picking strategy) as well as the weight of the loads to be handled. Design/methodology/approach: A model to select the best environmental MHE for inbound logistic activities has been developed. Environmental performance of the MHE has been evaluated in terms of carbon Footprint (CF). The model is tested with a tool adopting a VBA macro as well as a simulation software allowing the evaluation of energy and time required by the forklift in each phase of the material handling cycle: picking, sorting and storing of the items. Findings: Nowadays, it is not possible to identify ‘a priori’ a particular engine equipped forklift performing better than others under an environmental perspective. Consistently, the application of the developed model allows to identify the best MHE tailored to each case analyzed. Originality/value: This work gives a contribution to the disagreement between environmental performances of forklifts equipped with different engines. The developed model can be considered a valid support for decision makers to identify the best MHE minimizing the carbon footprint of inbound logistic activities.

  13. Minimizing the carbon footprint of material handling equipment: Comparison of electric and LPG forklifts

    International Nuclear Information System (INIS)

    Facchini, F.; Mummolo, G.; Mossa, G.; Digiesi, S.; Boenzi, F.; Verriello, R.

    2016-01-01

    Purpose: The aim of this study is to identify the best Material Handling Equipment (MHE) to minimize the carbon footprint of inbound logistic activities, based on the type of the warehouse (layout, facilities and order-picking strategy) as well as the weight of the loads to be handled. Design/methodology/approach: A model to select the best environmental MHE for inbound logistic activities has been developed. Environmental performance of the MHE has been evaluated in terms of carbon Footprint (CF). The model is tested with a tool adopting a VBA macro as well as a simulation software allowing the evaluation of energy and time required by the forklift in each phase of the material handling cycle: picking, sorting and storing of the items. Findings: Nowadays, it is not possible to identify ‘a priori’ a particular engine equipped forklift performing better than others under an environmental perspective. Consistently, the application of the developed model allows to identify the best MHE tailored to each case analyzed. Originality/value: This work gives a contribution to the disagreement between environmental performances of forklifts equipped with different engines. The developed model can be considered a valid support for decision makers to identify the best MHE minimizing the carbon footprint of inbound logistic activities.

  14. Minimizing the carbon footprint of material handling equipment: Comparison of electric and LPG forklifts

    Directory of Open Access Journals (Sweden)

    Francesco Facchini

    2016-12-01

    Full Text Available Purpose: The aim of this study is to identify the best Material Handling Equipment (MHE to minimize the carbon footprint of inbound logistic activities, based on the type of the warehouse (layout, facilities and order-picking strategy as well as the weight of the loads to be handled. Design/methodology/approach: A model to select the best environmental MHE for inbound logistic activities has been developed. Environmental performance of the MHE has been evaluated in terms of carbon Footprint (CF. The model is tested with a tool adopting a VBA macro as well as a simulation software allowing the evaluation of energy and time required by the forklift in each phase of the material handling cycle: picking, sorting and storing of the items. Findings: Nowadays, it is not possible to identify ‘a priori’ a particular engine equipped forklift performing better than others under an environmental perspective. Consistently, the application of the developed model allows to identify the best MHE tailored to each case analyzed.   Originality/value: This work gives a contribution to the disagreement between environmental performances of forklifts equipped with different engines. The developed model can be considered a valid support for decision makers to identify the best MHE minimizing the carbon footprint of inbound logistic activities.

  15. A case study on determining air monitoring requirements in a radioactive materials handling area

    International Nuclear Information System (INIS)

    Newton, G.J.; Bechtold, W.E.; Hoover, M.D.; Ghanbari, F.; Herring, P.S.; Jow, Hong-Nian

    1993-01-01

    A technical, defensible basis for the number and placement of air sampling instruments in a radioactive materials handling facility was developed. Historical air sampling data, process and physicochemical knowledge, qualitative smoke dispersion studies with video documentation, and quantitative trace gas dispersion studies were used to develop a strategy for number and placement of air samplers. These approaches can be used in other facilities to provide a basis for operational decisions. The requirements for retrospective sampling, personal sampling, and real-time monitoring are included. Other relevant operational decisions include selecting the numbers, placement, and appropriate sampling rates for instruments, identifying areas of stagnation or recirculation, and determining the adequacy and efficiency of any sampling transport lines. Justification is presented for using a graded approach to characterizing the workplace and determining air sampling and monitoring needs

  16. Risk assessment of manual material handling activities (case study: PT BRS Standard Industry)

    Science.gov (United States)

    Deviani; Triyanti, V.

    2017-12-01

    The process of moving material manually has the potential for injury to workers. The risk of injury will increase if we do not pay attention to the working conditions. The purpose of this study is to assess and analyze the injury risk level in manual handling material activity, as well as to improve the condition. The observed manual material handling activities is pole lifting and goods loading. These activities were analyzed using Job Strain Index method, Rapid Entire Body Assessment, and Chaffin’s 2D Planar Static Model. The results show that most workers who perform almost all activities have a high level of risk level with the score of JSI and REBA exceeds 9 points. For some activities, the estimated compression forces in the lumbar area also exceed the standard limits of 3400 N. Concerning this condition, several suggestions for improvement were made, improving the composition of packing, improving body posture, and making guideline posters.

  17. Biomechanical differences between expert and novice workers in a manual material handling task.

    Science.gov (United States)

    Plamondon, Andre; Denis, Denys; Delisle, Alain; Lariviere, Christian; Salazar, Erik

    2010-10-01

    The objective was to verify whether the methods were safer and more efficient when used by expert handlers than by novice handlers. Altogether, 15 expert and 15 novice handlers were recruited. Their task was to transfer four boxes from a conveyor to a hand trolley. Different characteristics of the load and lifting heights were modified to achieve a larger variety of methods by the participants. The results show that the net moments at the L5/S1 joint were not significantly different (p > 0.05) for the two groups. However, compared with the novices, the experts bent their lumbar region less (experts 54° (SD 11°); novices 66° (SD 15°)) but bent their knees more (experts approx. 72° (SD approx. 30°); novices approx. 53° (SD approx. 33°), which brought them closer to the box. The handler's posture therefore seems to be a major aspect that should be paid specific attention, mainly when there is maximum back loading. STATEMENT OF RELEVANCE: The findings of this research will be useful for improving manual material handling training programmes. Most biomechanical research is based on novice workers and adding information about the approach used by expert handlers in performing their tasks will help provide new avenues for reducing the risk of injury caused by this demanding physical task.

  18. Measurement of particulate concentrations produced during bulk material handling at the Tarragona harbor

    Energy Technology Data Exchange (ETDEWEB)

    Artinano, B.; Gomez-Moreno, F.J.; Pujadas, M.; Moreno, N.; Alastuey, A.; Querol, X.; Martin, F.; Guerra, A.; Luaces, J.A.; Basora, J. [CIEMAT, Madrid (Spain)

    2007-09-15

    Bulk material handling can be a significant source of particles in harbor areas. The atmospheric impact of a number of loading/unloading activities of diverse raw materials has been assessed from continuous measurements of ambient particle concentrations recorded close to the emission sources. Two experimental campaigns have been carried out in the Tarragona port to document the impact of specific handling operations and bulk materials. Dusty bulk materials such as silica-manganese powder, tapioca, coal, clinker and lucerne were dealt with during the experiments. The highest impacts on ambient particle concentrations were recorded during handling of clinker. For this material and silica-manganese powder, high concentrations were recorded in the fine grain size ({lt}2.5 {mu}m). The lowest impacts on particulate matter concentrations were recorded during handling of tapioca and lucerne, mainly in the coarse grain size (2-5-10 {mu} m). The effectiveness of several emission abatement measures, such as ground watering to diminish coal particle resuspension, was demonstrated to reduce ambient concentrations by up to two orders of magnitude. The importance of other good practices in specific handling operations, such as controlling the height of the shovel discharge, was also evidenced by these experiments. The results obtained can be further utilized as a useful experimental database for emission factor estimations.

  19. Ergonomics intervention in a tile industry- case of manual material handling.

    Science.gov (United States)

    Dormohammadi, Ali; Amjad Sardrudi, Hosein; Motamedzade, Majid; Dormohammadi, Reza; Musavi, Saeed

    2012-12-13

    Manual material handling is one of the major health and safety hazards in industry. This study aims to assess the lifting tasks, before and after intervention using NIOSH lifting equation and Manual Handling Assessment Charts (MAC). This interventional study was performed in 2011 in a tile manufacturing industry in Hamadan, located in the West of Iran. The prevalence of musculoskeletal discomfort was determined using Nordic musculoskeletal questionnaire. In order to assess the risk factors related to lifting and identify the high-risk activities, MAC and NIOSH lifting equation were used. In intervention phase, we designed a load-carrying cart with shelves capable of moving vertically up and down, similar to scissor lifts. After intervention, the reassessment of risk factors was conducted to determine the success of the intervention and to compare risk levels before and after intervention using t-test. The outputs of MAC and NIOSH lifting equation assessments before intervention revealed that all activities were at high-risk level. After intervention, the risk level decreased to average level. In conclusion, the results of intervention revealed a considerable decrease in risk level. It may be concluded that the given intervention was acceptable and favorably effective in preventing musculoskeletal disorders especially low back pain.

  20. 30 CFR 250.108 - What requirements must I follow for cranes and other material-handling equipment?

    Science.gov (United States)

    2010-07-01

    ... 30 Mineral Resources 2 2010-07-01 2010-07-01 false What requirements must I follow for cranes and... Performance Standards § 250.108 What requirements must I follow for cranes and other material-handling equipment? (a) All cranes installed on fixed platforms must be operated in accordance with American...

  1. Musculoskeletal symptoms associated with posterior load carriage: An assessment of manual material handling workers in Indonesia.

    Science.gov (United States)

    Muslim, Khoirul; Nussbaum, Maury A

    2015-06-05

    Concerns have been raised regarding the high prevalence of musculoskeletal symptoms (MSS) among manual material handling (MMH) workers. However, limited investigations have been undertaken among one large group of workers using a particular MMH method called posterior load carriage (PLC). This is typically done without the use of a backpack in developing countries, and involves exposure to known risk factors for MSS such as heavy loads, non-neutral postures, and high levels of repetition. To 1) determine the types and prevalence of MSS among PLC workers and the impacts of these MSS on workers, 2) explore job demands potentially contributing to MSS, and (3) obtain input from workers regarding possible improvements to facilitate future interventions. Structured interview applied to 108 workers to assess PLC worker characteristics and job demands in eight cities in Indonesia. MSS were reported in all anatomical regions evaluated, with symptoms most commonly reported at the lower back (72.2%), feet (69.4%), knees (64%), shoulders (47.2%), and neck (41.7%). Logistic regression indicated that MSS in the lower back were associated with longer work hours/day, MSS in the hands were associated with load mass, and MSS in the ankles/feet were associated with stature and load carriage frequency. MSS were reported to interfere with daily activity, but only few workers sought medical treatment. Possible improvements included the use of a belt, hook, or backpack/frame, and changes in the carriage method. The study suggests that PLC workers incur a relatively high MSS burden. Future studies are needed to develop and evaluate practical interventions and specific guidelines to improve working conditions and occupational health and safety for PLC workers.

  2. Analysis of manual material handling activity to increase work productivity (Case study: manufacturing company

    Directory of Open Access Journals (Sweden)

    Suryoputro Muhammad Ragil

    2018-01-01

    Full Text Available Manual material handling is one of work activities that have an effect on the physical aspect of workers in manufacturing industry, it is necessary to do the analysis of the risks from such activities. Analysis was performed on worker when performing manual lifting activity and when using two tools (Automatic Handlift and Manual Handlift. In addition to analyse in ergonomics aspect, time study analysis and productivity measurement were carried out to determine the effects of the equipment. Nordic Body Map (NBM questionnaire on worker using Automatic Handlift showed declining level of musculoskeletal disorders by 22%. REBA method obtained score of 10 and was declined to 4 after using these tool. The results of MPL method showed declining of Fc value from 4756.37 N to 1346.56 N. The results of RWL method showed declining of LI value (Lifting Index origin and destination from 1.84 and 1.18 to 1.12 and 0.89 respectively. As for worker using Manual Handlift, NBM questionnaire result shows declining level of musculoskeletal disorders by 57%. REBA method obtained scores of 8 and was reduced to 5. For MPL methode result, the Fc value is 4906.99 N and reduced to 2047.88 N. RWL method results showed declining of LI value (Lifting Index origin and destination from 1.02 and 0.67 to 0.74 and 0.58. The results of time study analysis showed declining of Standard Time when use the two tools and make the increasing productivity of 9% by worker using Automatic Hadlift and 4% by worker using Manual Handlift.

  3. Comparison of Customer Preference for Bulk Material Handling Equipment through Fuzzy-AHP Approach

    Science.gov (United States)

    Sen, Kingshuk; Ghosh, Surojit; Sarkar, Bijan

    2017-06-01

    In the present study, customer's perception has played one of the important roles for selection of the exact equipment out of available alternatives. The present study is dealt with the method of optimization of selection criteria of a material handling equipment, based on the technical specifications considered to be available at the user end. In this work, the needs of customers have been identified and prioritized, that lead to the selection of number of criteria, which have direct effect upon the performance of the equipment. To check the consistency of selection criteria, first of all an AHP based methodology is adopted with the identified criteria and available product categories, based upon which, the judgments of the users are defined to derive the priority scales. Such judgments expressed the relative strength or intensity of the impact of the elements of the hierarchy. Subsequently, all the alternatives have ranked for each identified criteria with subsequent constitution of weighted matrices. The same has been compared with the normalized values of approximate selling prices of the equipments to determine individual cost-benefit ratio. Based on the cost-benefit ratio, the equipment is ranked. With same conditions, the study is obtained again with a Fuzzy AHP concept, where a fuzzy linguistic approach has reduced the amount of uncertainty in decision making, caused by conventional AHP due to lack of deterministic approach. The priority vectors of category and criteria are determined separately and multiplied to obtain composite score. Subsequently, the average of fuzzy weights was determined and the preferences of equipment are ranked.

  4. How do low/high height and weight variation affect upper limb movements during manual material handling of industrial boxes?

    Directory of Open Access Journals (Sweden)

    Ana B. Oliveira

    Full Text Available OBJECTIVES: To evaluate the effect of surface height and load weight on upper limb movements and electromyographic (EMG recordings during manual handling performed by both experienced and inexperienced lifter subjects. METHODS: Sixteen experienced and sixteen inexperienced lifters handled a box (both 7 and 15 kg from an intermediate height (waist level to either a high or low surface. Electromyography and video images were recorded during the tasks. The 10th, 50th and 90th percentiles were calculated for the deltoid and biceps muscles, shoulder flexion, shoulder abduction, and elbow flexion movements. Groups, right/left sides, weights and heights were compared. There were no differences between either groups or sides. RESULTS: Weight and height variations affected EMG and posture, although weight had more impact on EMG. Shoulder abduction and flexion movements higher than 60º occurred, particularly for the higher surface. Shoulder flexion was also higher when the box was moved to the low height. This study provides new evidence as shoulder postures during boxes handling on low surfaces had not previously been evaluated. CONCLUSIONS: The high demand of upper limb in manual material handling tasks is clear, particularly for the shoulder. This knowledge can be used by physical therapists to plan better rehabilitation programs for manual material handling-related disorders, particularly focusing on return to work.

  5. PERANCANGAN MEJA ADJUSTABLE DENGAN MEMPERHATIKAN POSTUR KERJA PEKERJA MANUAL MATERIAL HANDLING (Studi Kasus di PT. Coca – Cola Bottling Indonesia

    Directory of Open Access Journals (Sweden)

    Singgih Saptadi

    2012-02-01

    Full Text Available PT Coca Cola Bottling Indonesia adalah salah satu perusahaan yang bergerak dalam bidang minuman ringan baik berupa carbonated soft drink (coca-cola, fanta, sprite maupun non-carbonated soft drink (frestea, ades. Penanganan material pada pabrik ini masih dilakukan secara manual (Manual Material Handling/MMH. Pekerjaan ini dimulai dengan pengangkatan kontainer dari atas conveyor kemudian dipindahkan dan disusun di atas pallet (proses palletizing.Rapid Entire Body Assessment (REBA adalah salah satu metode yang digunakan untuk menilai postur kerjakaitanya dengan cedera otot akibat bekerja. Pada metode ini juga dipengaruhi oleh faktor coupling, beban eksternal serta aktivitas pekerja yang dialami oleh subjek pengamatan. Implementasi REBA dilakukan pada postur penanganan material khususnya pada pengangkatan krat dan penyusunan krat. Dari hasil penilaian menunjukkan action level didominasi pada level 3 dan level 4, untuk itu perlu dilakukan tindakan perbaikan segera/saat itu juga. Dalam melakukan rekomendasi perbaikan postur penulis melakukan perancangan fasilitas yang disesuaikan dengan data antropometri dan didasarkan pada jenis aktifitasnya serta memberikan usulan fasilitas berupa meja adjustable. Penulis memvisualisasikan hasil rancangan melalui sofware 3DS max, dimana sofware ini juga digunakan sebagai analisis postur pada fasilitas kerja usulan. Kata kunci : Manual Material Handling, REBA, perancangan fasilitas kerja, meja adjustable, 3DS max                Coca Cola Bottling Indonesia is one of the company which move on soft drink areas like carbonated soft drinks (coca-cola, fanta, sprite or non-carbonated soft drinks (frestea,ades. Material handling in this factory is still done manually. This work is started by lifting the container from conveyor  then move it and arrange it above the pallet ( proses palletizing.              Rapid Entire Body Assesment ( REBA is one of the method by which is used to score working

  6. Machine assembly with a new material handling mechanism in the sewing machine

    Directory of Open Access Journals (Sweden)

    Umarova Z.M.

    2017-05-01

    Full Text Available the paper presents the dynamic model of the machine assembly with a recommended mechanism for moving material and the definition of the law of rails motion under various system parameters. The author has suggested the solution implemented by the system of differential equations numerically on the PC and the system describing the motion of the machine set. Recommended values ​​of the parameters of elastic links of material transfer mechanism have been obtained. The researcher has developed the methods of kinematic and dynamic analysis of the material transfer mechanism with elastic elements of the sewing machine and has approved the parameters and development of the design.

  7. The development of a model to predict the effects of worker and task factors on foot placements in manual material handling tasks.

    Science.gov (United States)

    Wagner, David W; Reed, Matthew P; Chaffin, Don B

    2010-11-01

    Accurate prediction of foot placements in relation to hand locations during manual materials handling tasks is critical for prospective biomechanical analysis. To address this need, the effects of lifting task conditions and anthropometric variables on foot placements were studied in a laboratory experiment. In total, 20 men and women performed two-handed object transfers that required them to walk to a shelf, lift an object from the shelf at waist height and carry the object to a variety of locations. Five different changes in the direction of progression following the object pickup were used, ranging from 45° to 180° relative to the approach direction. Object weights of 1.0 kg, 4.5 kg, 13.6 kg were used. Whole-body motions were recorded using a 3-D optical retro-reflective marker-based camera system. A new parametric system for describing foot placements, the Quantitative Transition Classification System, was developed to facilitate the parameterisation of foot placement data. Foot placements chosen by the subjects during the transfer tasks appeared to facilitate a change in the whole-body direction of progression, in addition to aiding in performing the lift. Further analysis revealed that five different stepping behaviours accounted for 71% of the stepping patterns observed. More specifically, the most frequently observed behaviour revealed that the orientation of the lead foot during the actual lifting task was primarily affected by the amount of turn angle required after the lift (R(2) = 0.53). One surprising result was that the object mass (scaled by participant body mass) was not found to significantly affect any of the individual step placement parameters. Regression models were developed to predict the most prevalent step placements and are included in this paper to facilitate more accurate human motion simulations and ergonomics analyses of manual material lifting tasks. STATEMENT OF RELEVANCE: This study proposes a method for parameterising the steps

  8. Translation in cell-free systems

    International Nuclear Information System (INIS)

    Jagus, R.

    1987-01-01

    The simplest, unambiguous identification of a particular mRNA is the identification of its protein product. This can be established by translation of the mRNA of interest in a cell-free protein-synthesizing system. Messenger RNA protein product identification is important in the isolation of a particular mRNA species for cDNA cloning and in the identification of positive cDNA clones. The two high-activity translation systems in common use are those prepared from rabbit reticulocytes and from wheat germ. Both systems are easy to prepare, and both are available commercially. Each has advantages and disadvantages over the other and a choice between the two will depend on the type of mRNAs to be translated, the prejudices of experience, and availability. The main disadvantage of the reticulocyte system is that it requires removal of endogenous mRNA. However, this is a relatively simple procedure. The wheat germ system does not require removal of endogenous mRNA and may translate weakly initiating mRNAs more efficiently. However, ionic optima for translation in the wheat germ system are more sensitive to the nature and concentration of mRNA and may need to be determined for each template. The biggest problem with the use of the wheat germ system is its tendency to produce incomplete translation products due to premature termination

  9. Real-time software use in nuclear materials handling criticality safety control

    International Nuclear Information System (INIS)

    Huang, S.; Lappa, D.; Chiao, T.; Parrish, C.; Carlson, R.; Lewis, J.; Shikany, D.; Woo, H.

    1997-01-01

    This paper addresses the use of real-time software to assist handlers of fissionable nuclear material. We focus specifically on the issue of workstation mass limits, and the need for handlers to be aware of, and check against, those mass limits during material transfers. Here ''mass limits'' generally refer to criticality safety mass limits; however, in some instances, workstation mass limits for some materials may be governed by considerations other than criticality, e.g., fire or release consequence limitation. As a case study, we provide a simplified reliability comparison of the use of a manual two handler system with a software-assisted two handler system. We identify the interface points between software and handlers that are relevant to criticality safety

  10. Proposed master-slave and automated remote handling system for high-temperature gas-cooled reactor fuel refabrication

    International Nuclear Information System (INIS)

    Grundmann, J.G.

    1974-01-01

    The Oak Ridge National Laboratory's Thorium-Uranium Recycle Facility (TURF) will be used to develop High-Temperature Gas-Cooled Reactor (HTGR) fuel recycle technology which can be applied to future HTGR commercial fuel recycling plants. To achieve recycle capabilities it is necessary to develop an effective material handling system to remotely transport equipment and materials and to perform maintenance tasks within a hot cell facility. The TURF facility includes hot cells which contain remote material handling equipment. To extend the capabilities of this equipment, the development of a master-slave manipulator and a 3D-TV system is necessary. Additional work entails the development of computer controls to provide: automatic execution of tasks, automatic traverse of material handling equipment, automatic 3D-TV camera sighting, and computer monitoring of in-cell equipment positions to prevent accidental collisions. A prototype system which will be used in the development of the above capabilities is presented. (U.S.)

  11. SAF: the next generation process for radiotoxic material handling in the nuclear fuel industry

    International Nuclear Information System (INIS)

    Nyman, D.H.; Graham, R.A.

    1984-01-01

    In 1980 the Secure Automated Fabrication (SAF) Project was established with the goal to design, build, and operate a remote process for manufacturing breeder reactor fuel pins. The SAF line will be housed in the Fuels and Materials Examination Facility (FMEF) at the Hanford site. The fabrication system and supporting operations are designed for computer-controlled operation from a centralized control room. In addition to improved worker protection, remote and automated fuel fabrication operations will result in enhanced safeguards and accountability of fuel material, improved product quality, and increased productivity. Installation of the SAF line equipment has started. Qualification runs are scheduled to begin in 1986 with production commencing in 1987

  12. The Department of Energy's Rocky Flats Plant: A guide to record series useful for health related research. Volume 4: Production and materials handling

    International Nuclear Information System (INIS)

    1995-01-01

    This is the fourth in a series of seven volumes which constitute a guide to records of the Rocky Flats Plant useful for conducting health-related research. The primary purpose of Volume 4 is to describe record series pertaining to production and materials handling activities at the Department of Energy's (DOE) Rocky Flats Plant, now named the Rocky Flats Environmental Technology Site, near Denver, Colorado. History Associates Incorporated (HAI) prepared this guide as part of its work as the support services contractor for DOE's Epidemiologic Records Inventory Project. This introduction briefly describes the Epidemiologic Records Inventory Project and HAI's role in the project, provides a history of production and materials handling practices at Rocky Flats, and identifies organizations contributing to production and materials handling policies and activities. Other topics include the scope and arrangement of the guide and the organization to contact for access to these records

  13. Storage and Materials Handling

    Science.gov (United States)

    1979-09-01

    18 9 Beef, instant , dehydrated ..... Pkg 24 12 6 Beef, noodle , dehydrated ...... Pkg 24 12 6 Beef, vegetable, noodle , dehy- drated... noodle , dehydrated . Can 60 30 15 .............. Pkg 24 12 6 Condensed" ....................... Can 72 36 18 Cream of onion, instant , dehy- drated...6 Onion, instant , dehydrated ... Pkg 24 12 6 Ready to serve ................... Can 48 24 12 Tomato-vegetable w/ noodle , dehydrated

  14. Gender involvement in manual material handling (mmh) tasks in agriculture and technology intervention to mitigate the resulting musculoskeletal disorders.

    Science.gov (United States)

    Singh, Suman; Sinwal, Neelima; Rathore, Hemu

    2012-01-01

    The lifting and carrying of loads in agriculture on small landholdings are unavoidable. Rural communities often lack access to appropriate technologies which may result in various health hazards. The objective was to study gender participation in agricultural activities involving manual material handling tasks, to assess MSDs experienced in various MMH tasks and to evaluate traditional method and designed technology. The study was conducted on 100 agricultural workers. Data on gender participation in MMH tasks in household, animal husbandry and agriculture and resulting MSDs was gathered. Pre and post assessment of technology intervention was done for NIOSH Lifting Index, QEC, and RPE. The results revealed greater susceptibility of females to musculoskeletal problems in most of the household and animal husbandry tasks. The hand trucks designed were pushing type with power grasp handle. The respondents were advised to carry 5 kg of weight per lift instead of lifting more weight in one lift/minute while filling the hand truck. By decreasing the weight and increasing the number of lifts per minute the respondents were seen falling in green zone indicating significant reduction in NIOSH lifting index. QEC scores concluded that for filling the hand truck 5 kg of weight should be carried to keep the exposure level low.

  15. Universal machine ''Shtrek'' and the tractor-lifter with pneumatic-equipment control. [Auxiliary multipurpose materials handling equipment

    Energy Technology Data Exchange (ETDEWEB)

    Bal' bert, B M; Borumenskiy, V A; Lishenko, A P; Mitchenko, G A

    1982-01-01

    The machine ''Shtrek'' is described. It makes it possible to mechanize over 20 auxiliary operations: loading-unloading operations: extraction of old and deformed timbering; dissmantling of obstructions; erection of different types of timbering; making and restoring of drainage channels; laying and straightening of a drift and its leveling; assembly and disassembly of pipelines and mine equipment, etc. Depending on the type of operation, the machine has the corresponding suspended equipment. The elementary variant has a limited area of application at mines of the central region of the Dunbass. Currently a pneumatic variant of the machine ''Shtrek'' has been developed. The electric motor and the starter of the pumping equipment of the machine have been replaced by a pneumatic motor and pneumatically controlled valve KTM-50. In this case there was significant reduction in the weight of the pumping equipment and in its overall dimensions; the electric drive of the hydraulic distributors for controlling the mechanisms were replaced by simpler pneumatic ones; the logical circuit of the control system was constructed on the USEPPA elements. A specialized tractor-lifter designed for moving suspended loads is described for auxiliary operations in the near-face zone of the preparatory drifts. The machine also lifts and lowers the boom, rotates the boom by 270/sup 0/ and additionally lifts and lowers the weight-lifting hook.

  16. Relative importance of expertise, lifting height and weight lifted on posture and lumbar external loading during a transfer task in manual material handling.

    Science.gov (United States)

    Plamondon, André; Larivière, Christian; Delisle, Alain; Denis, Denys; Gagnon, Denis

    2012-01-01

    The objective of this study was to measure the effect size of three important factors in manual material handling, namely expertise, lifting height and weight lifted. The effect of expertise was evaluated by contrasting 15 expert and 15 novice handlers, the effect of the weight lifted with a 15-kg box and a 23-kg box and the effect of lifting height with two different box heights: ground level and a 32 cm height. The task consisted of transferring a series of boxes from a conveyor to a hand trolley. Lifting height and weight lifted had more effect size than expertise on external back loading variables (moments) while expertise had low impact. On the other hand, expertise showed a significant effect of posture variables on the lumbar spine and knees. All three factors are important, but for a reduction of external back loading, the focus should be on the lifting height and weight lifted. The objective was to measure the effect size of three important factors in a transfer of boxes from a conveyor to a hand trolley. Lifting height and weight lifted had more effect size than expertise on external back loading variables but expertise was a major determinant in back posture.

  17. Signal transduction in cells of the immune system in microgravity

    Directory of Open Access Journals (Sweden)

    Huber Kathrin

    2008-10-01

    Full Text Available Abstract Life on Earth developed in the presence and under the constant influence of gravity. Gravity has been present during the entire evolution, from the first organic molecule to mammals and humans. Modern research revealed clearly that gravity is important, probably indispensable for the function of living systems, from unicellular organisms to men. Thus, gravity research is no more or less a fundamental question about the conditions of life on Earth. Since the first space missions and supported thereafter by a multitude of space and ground-based experiments, it is well known that immune cell function is severely suppressed in microgravity, which renders the cells of the immune system an ideal model organism to investigate the influence of gravity on the cellular and molecular level. Here we review the current knowledge about the question, if and how cellular signal transduction depends on the existence of gravity, with special focus on cells of the immune system. Since immune cell function is fundamental to keep the organism under imnological surveillance during the defence against pathogens, to investigate the effects and possible molecular mechanisms of altered gravity is indispensable for long-term space flights to Earth Moon or Mars. Thus, understanding the impact of gravity on cellular functions on Earth will provide not only important informations about the development of life on Earth, but also for therapeutic and preventive strategies to cope successfully with medical problems during space exploration.

  18. Reconstituted AIM2 inflammasome in cell-free system.

    Science.gov (United States)

    Kaneko, Naoe; Ito, Yuki; Iwasaki, Tomoyuki; Takeda, Hiroyuki; Sawasaki, Tatsuya; Migita, Kiyoshi; Agematsu, Kazunaga; Kawakami, Atsushi; Morikawa, Shinnosuke; Mokuda, Sho; Kurata, Mie; Masumoto, Junya

    2015-11-01

    Absent in melanoma 2 (AIM2) is an intracellular pattern-recognition receptor, which is a member of the PYHIN protein family, consisting of a PYD domain and an IFN-inducible nuclear localization (HIN) domain. AIM2 is reported to oligomerize with adaptor protein ASC upon sensing bacterial and viral cytosolic DNA in order to form the AIM2 inflammasome, which activates caspase-1 leading to IL-1β secretion. Dysregulation of AIM2 inflammasome is supposed to result in autoinflammatory and autoimmune diseases. Thus, the development of new targeted drugs against AIM2 inflammasome would be important for the treatment of these diseases. However, since AIM2 inflammasome is an intracellular receptor, enforced internalization of both ligands and candidate molecules is necessary for the screening of AIM2-inflammasome-targeted molecules. We developed a reconstituted AIM2 inflammasome in a cell-free system with amplified luminescent proximity homogeneous assay (Alpha). Strong Alpha signal was detected upon incubation with poly-deoxyadenylic-deoxythymidylic acid, poly(dA:dT), whereas no Alpha signal was detected upon incubation with muramyl dipeptide, one of the NLR ligands of Nod2 ligand. The interaction between AIM2 and ASC was disrupted by an anti-human ASC monoclonal antibody, CRID3, a class of diarylsulfonylurea-containing compounds, and glycyrrhizin, a substance found in liquorice root. Thus, the reconstituted AIM2 inflammasome in a cell-free system is useful for screening AIM2-inflammasome-targeted therapeutic molecules. Copyright © 2015 Elsevier B.V. All rights reserved.

  19. The development of in-cell remote inspection system in Tokai reprocessing plant

    International Nuclear Information System (INIS)

    Ishibashi, Yuzo

    1985-01-01

    In the Tokai fuel reprocessing plant, the containment is triple, i.e. the vessel containing radioactive material, then the concrete cell structure and finally the housing building. The fuel reprocessing plant is now proceeding with the development of an in-cell remote inspection system. The inspection system is for inspection of the cell itself and the equipment etc. in the cell, concerning the integrity. Described are the following: the course taken and problems in development of the remote inspection system; development of the floor rambling type remote inspection equipment and the multiple armed type, both for inspection of in-cell ''drip trays''; in-cell equipment inspection devices in specifications etc.; problems in its future development. (Mori, K.)

  20. The design of in-cell crane handling systems for nuclear plants

    International Nuclear Information System (INIS)

    Hansford, S.M.; Scott, R.

    1992-01-01

    The reprocessing and waste management facilities at (BNFL's) British Nuclear Fuels Limited's Sellafield site make extensive use of crane handling systems. These range from conventional mechanical handling operations as used generally in industry to high integrity applications through to remote robotic handling operations in radiation environments. This paper describes the design methodologies developed for the design of crane systems for remote handling operations - in-cell crane systems. In most applications the in-cell crane systems are an integral part of the plant process equipment and reliable and safe operations are a key design parameter. Outlined are the techniques developed to achieve high levels of crane system availability for operations in hazardous radiation environments. These techniques are now well established and proven through many years of successful plant operation. A recent application of in-cell crane handling systems design for process duty application is described. The benefits of a systematic design approach and a functionally-based engineering organization are also highlighted. (author)

  1. First experience with particle-in-cell plasma physics code on ARM-based HPC systems

    Science.gov (United States)

    Sáez, Xavier; Soba, Alejandro; Sánchez, Edilberto; Mantsinen, Mervi; Mateo, Sergi; Cela, José M.; Castejón, Francisco

    2015-09-01

    In this work, we will explore the feasibility of porting a Particle-in-cell code (EUTERPE) to an ARM multi-core platform from the Mont-Blanc project. The used prototype is based on a system-on-chip Samsung Exynos 5 with an integrated GPU. It is the first prototype that could be used for High-Performance Computing (HPC), since it supports double precision and parallel programming languages.

  2. First experience with particle-in-cell plasma physics code on ARM-based HPC systems

    OpenAIRE

    Sáez, Xavier; Soba, Alejandro; Sánchez, Edilberto; Mantsinen, Mervi; Mateo, Sergio; Cela, José M.; Castejón, Francisco

    2015-01-01

    In this work, we will explore the feasibility of porting a Particle-in-cell code (EUTERPE) to an ARM multi-core platform from the Mont-Blanc project. The used prototype is based on a system-on-chip Samsung Exynos 5 with an integrated GPU. It is the first prototype that could be used for High-Performance Computing (HPC), since it supports double precision and parallel programming languages. The research leading to these results has received funding from the European Com- munity's Seventh...

  3. Modular spectral imaging system for discrimination of pigments in cells and microbial communities.

    Science.gov (United States)

    Polerecky, Lubos; Bissett, Andrew; Al-Najjar, Mohammad; Faerber, Paul; Osmers, Harald; Suci, Peter A; Stoodley, Paul; de Beer, Dirk

    2009-02-01

    Here we describe a spectral imaging system for minimally invasive identification, localization, and relative quantification of pigments in cells and microbial communities. The modularity of the system allows pigment detection on spatial scales ranging from the single-cell level to regions whose areas are several tens of square centimeters. For pigment identification in vivo absorption and/or autofluorescence spectra are used as the analytical signals. Along with the hardware, which is easy to transport and simple to assemble and allows rapid measurement, we describe newly developed software that allows highly sensitive and pigment-specific analyses of the hyperspectral data. We also propose and describe a number of applications of the system for microbial ecology, including identification of pigments in living cells and high-spatial-resolution imaging of pigments and the associated phototrophic groups in complex microbial communities, such as photosynthetic endolithic biofilms, microbial mats, and intertidal sediments. This system provides new possibilities for studying the role of spatial organization of microorganisms in the ecological functioning of complex benthic microbial communities or for noninvasively monitoring changes in the spatial organization and/or composition of a microbial community in response to changing environmental factors.

  4. Regulation of ICAM-1 in Cells of the Monocyte/Macrophage System in Microgravity

    Directory of Open Access Journals (Sweden)

    Katrin Paulsen

    2015-01-01

    Full Text Available Cells of the immune system are highly sensitive to altered gravity, and the monocyte as well as the macrophage function is proven to be impaired under microgravity conditions. In our study, we investigated the surface expression of ICAM-1 protein and expression of ICAM-1 mRNA in cells of the monocyte/macrophage system in microgravity during clinostat, parabolic flight, sounding rocket, and orbital experiments. In murine BV-2 microglial cells, we detected a downregulation of ICAM-1 expression in clinorotation experiments and a rapid and reversible downregulation in the microgravity phase of parabolic flight experiments. In contrast, ICAM-1 expression increased in macrophage-like differentiated human U937 cells during the microgravity phase of parabolic flights and in long-term microgravity provided by a 2D clinostat or during the orbital SIMBOX/Shenzhou-8 mission. In nondifferentiated U937 cells, no effect of microgravity on ICAM-1 expression could be observed during parabolic flight experiments. We conclude that disturbed immune function in microgravity could be a consequence of ICAM-1 modulation in the monocyte/macrophage system, which in turn could have a strong impact on the interaction with T lymphocytes and cell migration. Thus, ICAM-1 can be considered as a rapid-reacting and sustained gravity-regulated molecule in mammalian cells.

  5. Modification of antioxidant systems in cell walls of maize roots by different nitrogen sources

    International Nuclear Information System (INIS)

    Hadži-Tašković Šukalović V; Vuletić, M.; Marković, K.; Željko, Vučinić; Kravić, N.

    2016-01-01

    Antioxidant systems of maize root cell walls grown on different nitrogen sources were evaluated. Plants were grown on a medium containing only NO3- or the mixture of NO3-+NH4+, in a 2:1 ratio. Eleven-day old plants, two days after the initiation of lateral roots, were used for the experiments. Cell walls were isolated from lateral roots and primary root segments, 2-7 cm from tip to base, representing zones of intense or decreased growth rates, respectively. Protein content and the activity of enzymes peroxidase, malate dehydrogenase and ascorbate oxidase ionically or covalently bound to the walls, as well as cell wall phenolic content and antioxidant capacity, were determined. Cell walls of plants grown on mixed N possess more developed enzymatic antioxidant systems and lower non-enzymatic antioxidant defenses than cell walls grown on NO3-. Irrespective of N treatment, the activities of all studied enzymes and protein content were higher in cell walls of lateral compared to primary roots. Phenolic content of cell walls isolated from lateral roots was higher in NO3--grown than in mixed N grown plants. No significant differences could be observed in the isozyme patterns of cell wall peroxidases isolated from plants grown on different nutrient solution. Our results indicate that different N treatments modify the antioxidant systems of root cell walls. Treatment with NO3- resulted in an increase of constitutive phenolic content, while the combination of NO3-+NH4+ elevated the redox enzyme activities in root cell walls.

  6. High Molecular Weight Isoforms of Growth Hormone In Cells of the Immune System

    Science.gov (United States)

    Weigent, Douglas A.

    2013-01-01

    A substantial body of research exists to support the idea that cells of the immune system produce growth hormone (GH). However, the structure and mechanism of action of lymphocyte-derived GH continues to remain largely unknown. Here we present the results of Western analysis of whole cell extracts showing that different molecular weight isoforms of GH of approximately 100 kDa, 65 kDa, and 48 kDa can be detected in primary mouse cells of the immune system and in the mouse EL4 cell line. The identity of the 65 kDa and 48 kDa isoforms of GH were confirmed by mass spectrometry. The various isoforms were detected in both enriched T and B spleen cell populations. The large molecular weight isoform appears to reside primarily in the cytoplasm whereas the lower molecular weight 65 kDa and 48 kDa isoforms were detected primarily in the nucleus. These results also suggest that GH isoforms are induced by oxidative stress. In EL4 cells overexpressing GH, the expression of luciferase controlled by a promoter containing the antioxidant response element is increased almost three-fold above control. The data suggest that the induction of isoforms of the GH molecule in cells of the immune system may be an important mechanism of adaptation and/or protection of lymphoid cells under conditions of oxidative stress. PMID:21741628

  7. Modification of antioxidant systems in cell walls of maize roots by different nitrogen sources

    Energy Technology Data Exchange (ETDEWEB)

    Hadži-Tašković Šukalović V; Vuletić, M.; Marković, K.; Željko, Vučinić; Kravić, N.

    2016-07-01

    Antioxidant systems of maize root cell walls grown on different nitrogen sources were evaluated. Plants were grown on a medium containing only NO3- or the mixture of NO3-+NH4+, in a 2:1 ratio. Eleven-day old plants, two days after the initiation of lateral roots, were used for the experiments. Cell walls were isolated from lateral roots and primary root segments, 2-7 cm from tip to base, representing zones of intense or decreased growth rates, respectively. Protein content and the activity of enzymes peroxidase, malate dehydrogenase and ascorbate oxidase ionically or covalently bound to the walls, as well as cell wall phenolic content and antioxidant capacity, were determined. Cell walls of plants grown on mixed N possess more developed enzymatic antioxidant systems and lower non-enzymatic antioxidant defenses than cell walls grown on NO3-. Irrespective of N treatment, the activities of all studied enzymes and protein content were higher in cell walls of lateral compared to primary roots. Phenolic content of cell walls isolated from lateral roots was higher in NO3--grown than in mixed N grown plants. No significant differences could be observed in the isozyme patterns of cell wall peroxidases isolated from plants grown on different nutrient solution. Our results indicate that different N treatments modify the antioxidant systems of root cell walls. Treatment with NO3- resulted in an increase of constitutive phenolic content, while the combination of NO3-+NH4+ elevated the redox enzyme activities in root cell walls.

  8. Material Handling Equipment Evaluation for Crater Repair

    Science.gov (United States)

    2016-11-01

    lifting (www.mcneiluscompanies.com). Agricultural/Mechanical Industry. The final equipment solution investigated was a telescoping boom crane (see... crane include: • Trailer-tongue mounting would provide for self-contained lifting capabilities on the simplified volumetric mixer. • Some models are...jib crane could potentially be effective as integrating lifting capabilities with the current simplified volumetric mixer. Both options could be

  9. Air conditioner for radioactive material handling facility

    International Nuclear Information System (INIS)

    Tanaka, Takeaki.

    1991-01-01

    An air conditioner intakes open-air from an open-air intake port to remove sands and sea salt particles by air filters. Then, natural and artificial radioactive particles of less than 1 μm are removed by high performance particulate filters. After controlling the temperature by an air heater or an air cooler, air is sent to each of chambers in a facility under pressure elevation by a blower. In this case, glass fibers are used as the filter material for the high performance particulate filter, which has a performance of more than 99.97% for the particles of 0.3 μm grain size. Since this can sufficiently remove the natural radioactive materials intruded from the outside, a detection limit value in each of the chambers of the facility can be set 10 -13 to 10 -14 μci/cm 3 in respect of radiation control. Accordingly, radiation control can be conducted smoothly and appropriately. (I.N.)

  10. The development of remote repairing system, decontamination and in-cell remote inspection equipment

    International Nuclear Information System (INIS)

    Ishibashi, Yuzo; Toyoda, Osamu; Haginoya, Isao; Yamamoto, Ryuichi; Tanaka, Yasumasa

    1993-01-01

    PNC has been developing remote repair and inspection technologies for in-cell components in reprocessing Plants. In this report, several remote technologies such as remote dismantling and removal, decontamination, remote pipe maintenance and remote in-cell inspection equipment are described. (author)

  11. Rule-based modeling: a computational approach for studying biomolecular site dynamics in cell signaling systems

    Science.gov (United States)

    Chylek, Lily A.; Harris, Leonard A.; Tung, Chang-Shung; Faeder, James R.; Lopez, Carlos F.

    2013-01-01

    Rule-based modeling was developed to address the limitations of traditional approaches for modeling chemical kinetics in cell signaling systems. These systems consist of multiple interacting biomolecules (e.g., proteins), which themselves consist of multiple parts (e.g., domains, linear motifs, and sites of phosphorylation). Consequently, biomolecules that mediate information processing generally have the potential to interact in multiple ways, with the number of possible complexes and post-translational modification states tending to grow exponentially with the number of binary interactions considered. As a result, only large reaction networks capture all possible consequences of the molecular interactions that occur in a cell signaling system, which is problematic because traditional modeling approaches for chemical kinetics (e.g., ordinary differential equations) require explicit network specification. This problem is circumvented through representation of interactions in terms of local rules. With this approach, network specification is implicit and model specification is concise. Concise representation results in a coarse graining of chemical kinetics, which is introduced because all reactions implied by a rule inherit the rate law associated with that rule. Coarse graining can be appropriate if interactions are modular, and the coarseness of a model can be adjusted as needed. Rules can be specified using specialized model-specification languages, and recently developed tools designed for specification of rule-based models allow one to leverage powerful software engineering capabilities. A rule-based model comprises a set of rules, which can be processed by general-purpose simulation and analysis tools to achieve different objectives (e.g., to perform either a deterministic or stochastic simulation). PMID:24123887

  12. Rule-based modeling: a computational approach for studying biomolecular site dynamics in cell signaling systems.

    Science.gov (United States)

    Chylek, Lily A; Harris, Leonard A; Tung, Chang-Shung; Faeder, James R; Lopez, Carlos F; Hlavacek, William S

    2014-01-01

    Rule-based modeling was developed to address the limitations of traditional approaches for modeling chemical kinetics in cell signaling systems. These systems consist of multiple interacting biomolecules (e.g., proteins), which themselves consist of multiple parts (e.g., domains, linear motifs, and sites of phosphorylation). Consequently, biomolecules that mediate information processing generally have the potential to interact in multiple ways, with the number of possible complexes and posttranslational modification states tending to grow exponentially with the number of binary interactions considered. As a result, only large reaction networks capture all possible consequences of the molecular interactions that occur in a cell signaling system, which is problematic because traditional modeling approaches for chemical kinetics (e.g., ordinary differential equations) require explicit network specification. This problem is circumvented through representation of interactions in terms of local rules. With this approach, network specification is implicit and model specification is concise. Concise representation results in a coarse graining of chemical kinetics, which is introduced because all reactions implied by a rule inherit the rate law associated with that rule. Coarse graining can be appropriate if interactions are modular, and the coarseness of a model can be adjusted as needed. Rules can be specified using specialized model-specification languages, and recently developed tools designed for specification of rule-based models allow one to leverage powerful software engineering capabilities. A rule-based model comprises a set of rules, which can be processed by general-purpose simulation and analysis tools to achieve different objectives (e.g., to perform either a deterministic or stochastic simulation). © 2013 Wiley Periodicals, Inc.

  13. Integration between a sales support system and a simulation tool

    OpenAIRE

    Wahlström, Ola

    2005-01-01

    InstantPlanner is a sales support system for the material handling industry, visualizing and calculating designs faster and more correctly than other tools on the market. AutoMod is a world leading simulation tool used in the material handling industry to optimize and calculate appropriate configuration designs. Both applications are favorable in their own area provide a great platform for integration with the properties of fast designing, correct product calculations, great simulation capabi...

  14. Overhead remote handling systems for the process facility modifications project

    International Nuclear Information System (INIS)

    Wiesener, R.W.; Grover, D.L.

    1987-01-01

    Each of the cells in the process facility modifications (PFM) project complex is provided with a variety of general purpose remote handling equipment including bridge cranes, monorail hoist, bridge-mounted electromechanical manipulator (EMM) and an overhead robot used for high efficiency particulate air (HEPA) filter changeout. This equipment supplements master-slave manipulators (MSMs) located throughout the complex to provide an overall remote handling system capability. The overhead handling equipment is used for fuel and waste material handling operations throughout the process cells. The system also provides the capability for remote replacement of all in-cell process equipment which may fail or be replaced for upgrading during the lifetime of the facility

  15. The role of protective systems in cell homeostasis upon gamma radiation

    International Nuclear Information System (INIS)

    Zasuxina, Q.D.

    2002-01-01

    -treatment. Probably it is related to high activity of SOD in children after chronic radiation. Similar data about increased activity of SOD were obtained too by Durnov et al. (2000). We received unexpected results investigating the effect of trien on RAR formation. First at all we didn't find any influence of trien on the level of RAR: the level of RAR in cells was similar without and with trien-treatment. Secondly, RAR did not distinguish in repair-deficient cells in comparison with results for healthy donors. So we received the proofs that SOD didn't take part in the formation of RAR. Next part of our investigation were devoted to the comparison of trien effect and garlic extract (GE)-pretreatment on the viability of human cells after gamma-radiation and during RAR. GE decreased the number of killed cells, trien-increased this number. However trien didn't affect on the cell survival during RAR. Several mechanisms have been proposed to explain the mutagen-preventive effects of GE. These include modulation of enzyme activity, inhibition of DNA adduct formation, free-radical scavenging and effects on cell proliferation. GE can inhibit the nitrosation reaction in vitro, also decreased DNA methylation and the formation O6-G induced by some mutagens. GE stimulated the activity of glutathion peroxidase and enzymes involved in natural protection by free radicals in addition to SOD. The activity of SOD also increased by GE. In conclusion it seems that inhibition of SOD increases the cell sensibility to gamma-radiation. Inhibition of SOD does not affect the number of DNA breaks in lymphocytes of children living in regions with an increased level of radiation. The level of cell defence against gamma radiation is similar in the experiments with GE-pretreatment and during RAR

  16. Geometric Integration Of The Valsov-Maxwell System With A Variational Particle-in-cell Scheme

    International Nuclear Information System (INIS)

    Squire, J.; Qin, H.; Tang, W.M.

    2012-01-01

    A fully variational, unstructured, electromagnetic particle-in-cell integrator is developed for integration of the Vlasov-Maxwell equations. Using the formalism of Discrete Exterior Calculus [1], the field solver, interpolation scheme and particle advance algorithm are derived through minimization of a single discrete field theory action. As a consequence of ensuring that the action is invariant under discrete electromagnetic gauge transformations, the integrator exactly conserves Gauss's law.

  17. Geometric integration of the Vlasov-Maxwell system with a variational particle-in-cell scheme

    Energy Technology Data Exchange (ETDEWEB)

    Squire, J.; Tang, W. M. [Plasma Physics Laboratory, Princeton University, Princeton, New Jersey 08543 (United States); Qin, H. [Plasma Physics Laboratory, Princeton University, Princeton, New Jersey 08543 (United States); Department of Modern Physics, University of Science and Technology of China, Hefei, Anhui 230026 (China)

    2012-08-15

    A fully variational, unstructured, electromagnetic particle-in-cell integrator is developed for integration of the Vlasov-Maxwell equations. Using the formalism of discrete exterior calculus [Desbrun et al., e-print arXiv:math/0508341 (2005)], the field solver, interpolation scheme, and particle advance algorithm are derived through minimization of a single discrete field theory action. As a consequence of ensuring that the action is invariant under discrete electromagnetic gauge transformations, the integrator exactly conserves Gauss's law.

  18. Absence of regulation of tumor cholesterogenesis in cell-free synthesizing systems

    International Nuclear Information System (INIS)

    Azrolan, N.; Coleman, P.S.

    1986-01-01

    In tumors, cholesterol synthesis de novo is deregulated relative to normal tissues. But no previous study has demonstrated the decontrol of tumor cholesterogenesis with cell-free cytosolic systems. They have utilized a lipid synthesizing, post-mitochondrial supernatant system (PMS), with 14 C-citrate as substrate, to characterize the cholesterogenic pathway in Morris Hepatoma 3924A and normal rat liver. The rate of cholesterogenesis in the hepatoma PMS was 6-fold higher than that in the liver system on a per cell basis. The ratio of sterol-to-fatty acid synthesis was also significantly greater in the tumor versus the liver PMS. The authors determined the steady-state carbon flux through the early intermediates of the lipogenic pathways. Whereas the liver system displayed a metabolic crossover point at the HMG-CoA reductase reaction, the hepatoma system showed no evidence of control at this rate-limiting site of sterol synthesis. Furthermore, acetyl-CoA formation from added citrate (via ATP-citrate lyase) exhibited rates of 42% and 88% in excess of that required for lipidogenesis by liver and tumor PMS systems, respectively. Clearly, a cell-free PMS system from tumor tissue displays the property of deregulated lipidogenesis, especially cholesterol biosynthesis. The authors suggest that deregulated and continuously operating cholesterogenesis would provide for an increased level of a mevalonate-derived sterol pathway intermediate proposed as a trigger for DNA synthesis and cell proliferation in tumors

  19. New perspectives in cell delivery systems for tissue regeneration: natural-derived injectable hydrogels.

    Science.gov (United States)

    Munarin, Fabiola; Petrini, Paola; Bozzini, Sabrina; Tanzi, Maria Cristina

    2012-09-27

    Natural polymers, because of their biocompatibility, availability, and physico-chemical properties have been the materials of choice for the fabrication of injectable hydrogels for regenerative medicine. In particular, they are appealing materials for delivery systems and provide sustained and controlled release of drugs, proteins, gene, cells, and other active biomolecules immobilized.In this work, the use of hydrogels obtained from natural source polymers as cell delivery systems is discussed. These materials were investigated for the repair of cartilage, bone, adipose tissue, intervertebral disc, neural, and cardiac tissue. Papers from the last ten years were considered, with a particular focus on the advances of the last five years. A critical discussion is centered on new perspectives and challenges in the regeneration of specific tissues, with the aim of highlighting the limits of current systems and possible future advancements.

  20. Explicit high-order non-canonical symplectic particle-in-cell algorithms for Vlasov-Maxwell systems

    International Nuclear Information System (INIS)

    Xiao, Jianyuan; Liu, Jian; He, Yang; Zhang, Ruili; Qin, Hong; Sun, Yajuan

    2015-01-01

    Explicit high-order non-canonical symplectic particle-in-cell algorithms for classical particle-field systems governed by the Vlasov-Maxwell equations are developed. The algorithms conserve a discrete non-canonical symplectic structure derived from the Lagrangian of the particle-field system, which is naturally discrete in particles. The electromagnetic field is spatially discretized using the method of discrete exterior calculus with high-order interpolating differential forms for a cubic grid. The resulting time-domain Lagrangian assumes a non-canonical symplectic structure. It is also gauge invariant and conserves charge. The system is then solved using a structure-preserving splitting method discovered by He et al. [preprint http://arxiv.org/abs/arXiv:1505.06076 (2015)], which produces five exactly soluble sub-systems, and high-order structure-preserving algorithms follow by combinations. The explicit, high-order, and conservative nature of the algorithms is especially suitable for long-term simulations of particle-field systems with extremely large number of degrees of freedom on massively parallel supercomputers. The algorithms have been tested and verified by the two physics problems, i.e., the nonlinear Landau damping and the electron Bernstein wave

  1. Explicit high-order non-canonical symplectic particle-in-cell algorithms for Vlasov-Maxwell systems

    Energy Technology Data Exchange (ETDEWEB)

    Xiao, Jianyuan [School of Nuclear Science and Technology and Department of Modern Physics, University of Science and Technology of China, Hefei, Anhui 230026, China; Key Laboratory of Geospace Environment, CAS, Hefei, Anhui 230026, China; Qin, Hong [School of Nuclear Science and Technology and Department of Modern Physics, University of Science and Technology of China, Hefei, Anhui 230026, China; Plasma Physics Laboratory, Princeton University, Princeton, New Jersey 08543, USA; Liu, Jian [School of Nuclear Science and Technology and Department of Modern Physics, University of Science and Technology of China, Hefei, Anhui 230026, China; Key Laboratory of Geospace Environment, CAS, Hefei, Anhui 230026, China; He, Yang [School of Nuclear Science and Technology and Department of Modern Physics, University of Science and Technology of China, Hefei, Anhui 230026, China; Key Laboratory of Geospace Environment, CAS, Hefei, Anhui 230026, China; Zhang, Ruili [School of Nuclear Science and Technology and Department of Modern Physics, University of Science and Technology of China, Hefei, Anhui 230026, China; Key Laboratory of Geospace Environment, CAS, Hefei, Anhui 230026, China; Sun, Yajuan [LSEC, Academy of Mathematics and Systems Science, Chinese Academy of Sciences, P.O. Box 2719, Beijing 100190, China

    2015-11-01

    Explicit high-order non-canonical symplectic particle-in-cell algorithms for classical particle-field systems governed by the Vlasov-Maxwell equations are developed. The algorithms conserve a discrete non-canonical symplectic structure derived from the Lagrangian of the particle-field system, which is naturally discrete in particles. The electromagnetic field is spatially discretized using the method of discrete exterior calculus with high-order interpolating differential forms for a cubic grid. The resulting time-domain Lagrangian assumes a non-canonical symplectic structure. It is also gauge invariant and conserves charge. The system is then solved using a structure-preserving splitting method discovered by He et al. [preprint arXiv: 1505.06076 (2015)], which produces five exactly soluble sub-systems, and high-order structure-preserving algorithms follow by combinations. The explicit, high-order, and conservative nature of the algorithms is especially suitable for long-term simulations of particle-field systems with extremely large number of degrees of freedom on massively parallel supercomputers. The algorithms have been tested and verified by the two physics problems, i.e., the nonlinear Landau damping and the electron Bernstein wave. (C) 2015 AIP Publishing LLC.

  2. Spore coat protein synthesis in cell-free systems from sporulating cells of Bacillus subtilis.

    Science.gov (United States)

    Nakayama, T; Munoz, L E; Sadaie, Y; Doi, R H

    1978-09-01

    Cell-free systems for protein synthesis were prepared from Bacillus subtilis 168 cells at several stages of sporulation. Immunological methods were used to determine whether spore coat protein could be synthesized in the cell-free systems prepared from sporulating cells. Spore coat protein synthesis first occurred in extracts from stage t2 cells. The proportion of spore coat protein to total proteins synthesized in the cell-free systems was 2.4 and 3.9% at stages t2 and t4, respectively. The sodium dodecyl sulfate-urea-polyacrylamide gel electrophoresis patterns of immunoprecipitates from the cell-free systems showed the complete synthesis of an apparent spore coat protein precursor (molecular weight, 25,000). A polypeptide of this weight was previously identified in studies in vivo (L.E. Munoz, Y. Sadaie, and R.H. Doi, J. Biol. Chem., in press). The synthesis in vitro of polysome-associated nascent spore coat polypeptides with varying molecular weights up to 23,000 was also detected. These results indicate that the spore coat protein may be synthesized as a precursor protein. The removal of proteases in the crude extracts by treatment with hemoglobin-Sepharose affinity techniques may be preventing the conversion of the large 25,000-dalton precursor to the 12,500-dalton mature spore coat protein.

  3. An improved out-cell to in-cell rapid transfer system at the HFEF/South

    International Nuclear Information System (INIS)

    Bacca, J.P.; Sherman, E.K.

    1991-01-01

    This paper reports on Argonne National Laboratory's Fuel Cycle Facility (FCF) (formerly named Hot Fuel Examination Facility-South) (HFEF/South) which is currently being refurbished and upgraded in preparation for demonstrating remote, fast reactor metal-fuel reprocessing and refabrication, as part of the Integral Fast Reactor (IFR) Program. Among the FCF hot-cell system upgrades being provided is a newly fabricated, direct, out-of-cell to in-cell, small-item transfer system for the FCF argon cell. This system will enable the rapid transfer of selected small items from the hot cell exterior into the argon cell (argon-gas atmosphere) of the facility, without necessitating the use of formerly employed, very time-consuming, and quite laborious procedures. The new system will be especially valuable for the rapid insertion of IFR fuel processing makeup materials and small tools into the argon cell, and for use in argon cell and overall FCF radioactive contamination-control activities

  4. Reverse Genetics System Demonstrates that Rotavirus Nonstructural Protein NSP6 Is Not Essential for Viral Replication in Cell Culture.

    Science.gov (United States)

    Komoto, Satoshi; Kanai, Yuta; Fukuda, Saori; Kugita, Masanori; Kawagishi, Takahiro; Ito, Naoto; Sugiyama, Makoto; Matsuura, Yoshiharu; Kobayashi, Takeshi; Taniguchi, Koki

    2017-11-01

    The use of overlapping open reading frames (ORFs) to synthesize more than one unique protein from a single mRNA has been described for several viruses. Segment 11 of the rotavirus genome encodes two nonstructural proteins, NSP5 and NSP6. The NSP6 ORF is present in the vast majority of rotavirus strains, and therefore the NSP6 protein would be expected to have a function in viral replication. However, there is no direct evidence of its function or requirement in the viral replication cycle yet. Here, taking advantage of a recently established plasmid-only-based reverse genetics system that allows rescue of recombinant rotaviruses entirely from cloned cDNAs, we generated NSP6-deficient viruses to directly address its significance in the viral replication cycle. Viable recombinant NSP6-deficient viruses could be engineered. Single-step growth curves and plaque formation of the NSP6-deficient viruses confirmed that NSP6 expression is of limited significance for RVA replication in cell culture, although the NSP6 protein seemed to promote efficient virus growth. IMPORTANCE Rotavirus is one of the most important pathogens of severe diarrhea in young children worldwide. The rotavirus genome, consisting of 11 segments of double-stranded RNA, encodes six structural proteins (VP1 to VP4, VP6, and VP7) and six nonstructural proteins (NSP1 to NSP6). Although specific functions have been ascribed to each of the 12 viral proteins, the role of NSP6 in the viral replication cycle remains unknown. In this study, we demonstrated that the NSP6 protein is not essential for viral replication in cell culture by using a recently developed plasmid-only-based reverse genetics system. This reverse genetics approach will be successfully applied to answer questions of great interest regarding the roles of rotaviral proteins in replication and pathogenicity, which can hardly be addressed by conventional approaches. Copyright © 2017 American Society for Microbiology.

  5. Gap junctions in cells of the immune system: structure, regulation and possible functional roles

    Directory of Open Access Journals (Sweden)

    J.C. Sáez

    2000-04-01

    Full Text Available Gap junction channels are sites of cytoplasmic communication between contacting cells. In vertebrates, they consist of protein subunits denoted connexins (Cxs which are encoded by a gene family. According to their Cx composition, gap junction channels show different gating and permeability properties that define which ions and small molecules permeate them. Differences in Cx primary sequences suggest that channels composed of different Cxs are regulated differentially by intracellular pathways under specific physiological conditions. Functional roles of gap junction channels could be defined by the relative importance of permeant substances, resulting in coordination of electrical and/or metabolic cellular responses. Cells of the native and specific immune systems establish transient homo- and heterocellular contacts at various steps of the immune response. Morphological and functional studies reported during the last three decades have revealed that many intercellular contacts between cells in the immune response present gap junctions or "gap junction-like" structures. Partial characterization of the molecular composition of some of these plasma membrane structures and regulatory mechanisms that control them have been published recently. Studies designed to elucidate their physiological roles suggest that they might permit coordination of cellular events which favor the effective and timely response of the immune system.

  6. [Metabolic changes in cells under electromagnetic radiation of mobile communication systems].

    Science.gov (United States)

    Iakimenko, I L; Sidorik, E P; Tsybulin, A S

    2011-01-01

    Review is devoted to the analysis of biological effects of microwaves. The results of last years' researches indicated the potential risks of long-term low-level microwaves exposure for human health. The analysis of metabolic changes in living cells under the exposure of microwaves from mobile communication systems indicates that this factor is stressful for cells. Among the reproducible effects of low-level microwave radiation are overexpression of heat shock proteins, an increase of reactive oxygen species level, an increase of intracellular Ca2+, damage of DNA, inhibition of DNA reparation, and induction of apoptosis. Extracellular-signal-regulated kinases ERK and stress-related kinases p38MAPK are involved in metabolic changes. Analysis of current data suggests that the concept of exceptionally thermal mechanism of biological effects of microwaves is not correct. In turn, this raises the question of the need to revaluation of modern electromagnetic standards based on thermal effects of non-ionizing radiation on biological systems.

  7. A system for the rapid detection of bacterial contamination in cell-based therapeutica

    Science.gov (United States)

    Bolwien, Carsten; Erhardt, Christian; Sulz, Gerd; Thielecke, Hagen; Johann, Robert; Pudlas, Marieke; Mertsching, Heike; Koch, Steffen

    2010-02-01

    Monitoring the sterility of cell or tissue cultures is of major concern, particularly in the fields of regenerative medicine and tissue engineering when implanting cells into the human body. Our sterility-control system is based on a Raman micro-spectrometer and is able to perform fast sterility testing on microliters of liquid samples. In conventional sterility control, samples are incubated for weeks to proliferate the contaminants to concentrations above the detection limit of conventional analysis. By contrast, our system filters particles from the liquid sample. The filter chip fabricated in microsystem technology comprises a silicon nitride membrane with millions of sub-micrometer holes to retain particles of critical sizes and is embedded in a microfluidic cell specially suited for concomitant microscopic observation. After filtration, identification is carried out on the single particle level: image processing detects possible contaminants and prepares them for Raman spectroscopic analysis. A custom-built Raman-spectrometer-attachment coupled to the commercial microscope uses 532nm or 785nm Raman excitation and records spectra up to 3400cm-1. In the final step, the recorded spectrum of a single particle is compared to an extensive library of GMP-relevant organisms, and classification is carried out based on a support vector machine.

  8. Thermal proximity coaggregation for system-wide profiling of protein complex dynamics in cells.

    Science.gov (United States)

    Tan, Chris Soon Heng; Go, Ka Diam; Bisteau, Xavier; Dai, Lingyun; Yong, Chern Han; Prabhu, Nayana; Ozturk, Mert Burak; Lim, Yan Ting; Sreekumar, Lekshmy; Lengqvist, Johan; Tergaonkar, Vinay; Kaldis, Philipp; Sobota, Radoslaw M; Nordlund, Pär

    2018-03-09

    Proteins differentially interact with each other across cellular states and conditions, but an efficient proteome-wide strategy to monitor them is lacking. We report the application of thermal proximity coaggregation (TPCA) for high-throughput intracellular monitoring of protein complex dynamics. Significant TPCA signatures observed among well-validated protein-protein interactions correlate positively with interaction stoichiometry and are statistically observable in more than 350 annotated human protein complexes. Using TPCA, we identified many complexes without detectable differential protein expression, including chromatin-associated complexes, modulated in S phase of the cell cycle. Comparison of six cell lines by TPCA revealed cell-specific interactions even in fundamental cellular processes. TPCA constitutes an approach for system-wide studies of protein complexes in nonengineered cells and tissues and might be used to identify protein complexes that are modulated in diseases. Copyright © 2018 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works.

  9. In-cell protease assay systems based on trans-localizing molecular beacon proteins using HCV protease as a model system.

    Directory of Open Access Journals (Sweden)

    Jeong Hee Kim

    Full Text Available This study describes a sensitive in-cell protease detection system that enables direct fluorescence detection of a target protease and its inhibition inside living cells. This live-cell imaging system provides a fluorescent molecular beacon protein comprised of an intracellular translocation signal sequence, a protease-specific cleavage sequence, and a fluorescent tag sequence(s. The molecular beacon protein is designed to change its intracellular localization upon cleavage by a target protease, i.e., from the cytosol to a subcellular organelle or from a subcellular organelle to the cytosol. Protease activity can be monitored at the single cell level, and accordingly the entire cell population expressing the protease can be accurately enumerated. The clear cellular change in fluorescence pattern makes this system an ideal tool for various life science and drug discovery research, including high throughput and high content screening applications.

  10. Development of an automated guided vehicle controller using a systems engineering approach

    Directory of Open Access Journals (Sweden)

    Ferreira, Tremaine

    2016-08-01

    Full Text Available Automated guided vehicles (AGVs are widely used for transporting materials in industry and commerce. In this research, an intelligent AGV-based material-handling system was developed using a model- based systems engineering (MBSE approach. The core of the AGV, the controller, was designed in the system modelling language environment using Visual Paradigm software, and then implemented in the hardware. As the result, the AGV’s complex tasks of material handling, navigation, and communication were successfully accomplished and tested in the real industrial environment. The developed AGV is capable of towing trolleys with a weight of up to 200kg at walking speed. The AGV can be incorporated into an intelligent material-handling system with multiple autonomous vehicles and work stations, thus providing flexibility and reconfigurability for the whole manufacturing system. Ergonomic and safety aspects were also considered in the design of the AGV. A comprehensive safety system that is compliant with industrial standards was implemented.

  11. A system identification approach for developing model predictive controllers of antibody quality attributes in cell culture processes.

    Science.gov (United States)

    Downey, Brandon; Schmitt, John; Beller, Justin; Russell, Brian; Quach, Anthony; Hermann, Elizabeth; Lyon, David; Breit, Jeffrey

    2017-11-01

    As the biopharmaceutical industry evolves to include more diverse protein formats and processes, more robust control of Critical Quality Attributes (CQAs) is needed to maintain processing flexibility without compromising quality. Active control of CQAs has been demonstrated using model predictive control techniques, which allow development of processes which are robust against disturbances associated with raw material variability and other potentially flexible operating conditions. Wide adoption of model predictive control in biopharmaceutical cell culture processes has been hampered, however, in part due to the large amount of data and expertise required to make a predictive model of controlled CQAs, a requirement for model predictive control. Here we developed a highly automated, perfusion apparatus to systematically and efficiently generate predictive models using application of system identification approaches. We successfully created a predictive model of %galactosylation using data obtained by manipulating galactose concentration in the perfusion apparatus in serialized step change experiments. We then demonstrated the use of the model in a model predictive controller in a simulated control scenario to successfully achieve a %galactosylation set point in a simulated fed-batch culture. The automated model identification approach demonstrated here can potentially be generalized to many CQAs, and could be a more efficient, faster, and highly automated alternative to batch experiments for developing predictive models in cell culture processes, and allow the wider adoption of model predictive control in biopharmaceutical processes. © 2017 The Authors Biotechnology Progress published by Wiley Periodicals, Inc. on behalf of American Institute of Chemical Engineers Biotechnol. Prog., 33:1647-1661, 2017. © 2017 The Authors Biotechnology Progress published by Wiley Periodicals, Inc. on behalf of American Institute of Chemical Engineers.

  12. Development of the system for the estimation of materials flow in pyrochemical reprocessing plant. Characteristic evaluation of the oxide electrowinning plant

    International Nuclear Information System (INIS)

    Okamura, Nobuo; Tozawa, Katuhiro; Sato, Koji

    2002-07-01

    The operation of the plant with the non-aqueous reprocessing technology depends on the materials handling equipment closely. Because the value of decontamination factor of the products in the plant is low, treatment of nuclear materials requires remote operation technology. So the system for the evaluation of materials flow in the plant was built to evaluate the production ability of the plant and to check out the plant operation from the viewpoint of materials flow. The system is only based on information of the treatment abilities of materials handling machines and process installations and the arrangement of process installations in the reprocessing cell that influences a way to operate materials handling machines intensity. Therefore the system can be used to estimate the characteristics of non-aqueous plants that are not in detail design stage. The amount of production and the characteristics of the oxide electrowinning plant (operation term 200days/year, plant capacity 50tHM/year in design) designed in Feasibility Study Phase1 were estimated using the system. The results show that the practical amount of production of the plant design is about 88% of the designed value. To increase the amount of production, it is more useful to speed up materials handling machine time than to install new installation or to give priority to conduct bottleneck processes. It is because materials handling influences the production ability of the plant deeply. (author)

  13. Life cycle costs measurement of complex systems manufactured by an engineer-to-order company

    NARCIS (Netherlands)

    Öner, K.B.; Franssen, R.; Kiesmüller, G.P.; Houtum, van G.J.J.A.N.; Qui, R.G.; Russell, D.W.; Sullivan, W.G.

    2007-01-01

    Complex technical systems such as packaging lines, computer networks, material handling systems, are crucial for the operations at the companies (or institutions) where they are installed. Companies require high availability because their primary processes may halt when these systems are down. High

  14. A Multiple Sensor Machine Vision System for Automatic Hardwood Feature Detection

    Science.gov (United States)

    D. Earl Kline; Richard W. Conners; Daniel L. Schmoldt; Philip A. Araman; Robert L. Brisbin

    1993-01-01

    A multiple sensor machine vision prototype is being developed to scan full size hardwood lumber at industrial speeds for automatically detecting features such as knots holes, wane, stain, splits, checks, and color. The prototype integrates a multiple sensor imaging system, a materials handling system, a computer system, and application software. The prototype provides...

  15. Remote material handling in the Plutonium Immobilization Project. Revision 1

    International Nuclear Information System (INIS)

    Brault, J.R.

    2000-01-01

    With the downsizing of the US and Russian nuclear stockpiles, large quantities of weapons-usable plutonium in the US are being declared excess and will be disposed of by the Department of Energy Fissile Materials Disposition Program. To implement this program, DOE has selected the Savannah River Site (SRS) for the construction and operation of three new facilities: pit disassembly and conversion; mixed oxide fuel fabrication; and plutonium immobilization. The Plutonium Immobilization Project (PIP) will immobilize a portion of the excess plutonium in a hybrid ceramic and glass form containing high level waste for eventual disposal in a geologic repository. The PIP is divided into three distinct operating areas: Plutonium Conversion, First Stage Immobilization, and Second Stage Immobilization. Processing technology for the PIP is being developed jointly by the Lawrence Livermore National Laboratory and Westinghouse Savannah River Company. This paper will discuss development of the automated unpacking and sorting operations in the conversion area, and the automated puck and tray handling operations in the first stage immobilization area. Due to the high radiation levels and toxicity of the materials to be disposed of, the PIP will utilize automated equipment in a contained (glovebox) facility. Most operations involving plutonium-bearing materials will be performed remotely, separating personnel from the radiation source. Source term materials will be removed from the operations during maintenance. Maintenance will then be performed hands on within the containment using glove ports

  16. 29 CFR 1926.602 - Material handling equipment.

    Science.gov (United States)

    2010-07-01

    ... covered in Power Crane and Shovel Associations Standards No. 1 and No. 2 of 1968, and No. 3 of 1969, shall be complied with, and shall apply to cranes, machines, and attachments under this part. (c) Lifting... vertical and horizontal controls elevatable with the lifting carriage or forks for lifting personnel, the...

  17. Nuclear Technology Series. Course 25: Radioactive Material Handling Techniques.

    Science.gov (United States)

    Center for Occupational Research and Development, Inc., Waco, TX.

    This technical specialty course is one of thirty-five courses designed for use by two-year postsecondary institutions in five nuclear technician curriculum areas: (1) radiation protection technician, (2) nuclear instrumentation and control technician, (3) nuclear materials processing technician, (4) nuclear quality-assurance/quality-control…

  18. Accidents in manual material handling tasks in the construction industry

    OpenAIRE

    Azevedo, R.

    2011-01-01

    Tese de Doutoramento em Engenharia Civil A temática dos acidentes de trabalho na Construção tem sido alvo de investigação frequente, através da publicação de diversos estudos centrados nas causas e circunstâncias destes acidentes. Estes estudos são unânimes em considerar este sector como aquele que regista o maior número de acidentes mortais, estando o fenómeno mais comum associado a quedas em altura. Pese embora a importância dos acidentes por quedas em altura, quer pela...

  19. 41 CFR 101-25.405 - Materials handling equipment.

    Science.gov (United States)

    2010-07-01

    ... 50 45 40 30 25 20 15 10 Straddle truck 15 50 50 50 45 45 45 40 40 35 35 30 25 20 15 10 Electric Fork... percentage of acquisition costs (years in use) 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 Gasoline Fork truck (2000 pounds to 6000 pounds) 8 50 45 40 30 25 20 15 10 Fork truck (over 6000 pounds) 10 50 45 40 35 30 25 20 15...

  20. Integrating CAD/CAM in Automation and Materials Handling

    Science.gov (United States)

    Deal, Walter F.; Jones, Catherine E.

    2012-01-01

    Humans by their very nature are users of tools, materials, and processes as a part of their survival and existence. As humans have progressed over time, their civilizations and societies have changed beyond imagination and have moved from hunters and gatherers of food and materials for survival to sophisticated societies with complex social and…

  1. Utilizing Technology in Manual Material Handling and Safe Lifting.

    Science.gov (United States)

    Snyder, Mick

    2016-02-01

    There is great potential to decrease injuries with the use of these new technologies, especially musculoskeletal disorders and repetitive task-related injuries. Initial costs can be considerable for some of these units, but they are much cheaper than a back surgery. As with all technology, the first designs cost a small fortune, but as we are seeing even now, the pricing is decreasing and the quality is increasing for these devices. In 30 years, we might all have flying cars like "Back to the Future II" predicted we would in 2015 or be able to figure out a tricorder like on "Star Trek"! For more information on exoskeletons, exoskeletonreport.com is a great resource.

  2. 41 CFR 50-204.3 - Material handling and storage.

    Science.gov (United States)

    2010-07-01

    .... Bags, containers, bundles, etc. stored in tiers shall be stacked, blocked, interlocked and limited in... harborage. Vegetation control will be exercised when necessary. (d) Proper drainage shall be provided. (e...

  3. Design guides for radioactive-material-handling facilities and equipment

    International Nuclear Information System (INIS)

    Doman, D.R.; Barker, R.E.

    1980-01-01

    Fourteen key areas relating to facilities and equipment for handling radioactive materials involved in examination, reprocessing, fusion fuel handling and remote maintenance have been defined and writing groups established to prepare design guides for each areas. The guides will give guidance applicable to design, construction, operation, maintenance and safety, together with examples and checklists. Each guide will be reviewed by an independent review group. The guides are expected to be compiled and published as a single document

  4. 29 CFR 1926.251 - Rigging equipment for material handling.

    Science.gov (United States)

    2010-07-01

    ..., shall consist of one continuous piece without knot or splice. (iii) Eyes in wire rope bridles, slings... splices shall contain at least three full tucks, and short splices shall contain at least six full tucks... tails shall project at least six rope diameters beyond the last full tuck. For fiber ropes 1-inch...

  5. Coordinated Mobile Manipulation for Robotics Material Handling, Phase II

    Data.gov (United States)

    National Aeronautics and Space Administration — Robots will play an important role in NASA's exploration activities over the next several decades. They will land on the Lunar surface ahead of humans and help...

  6. Processing device for discharged water from radioactive material handling facility

    International Nuclear Information System (INIS)

    Kono, Takao; Kono, Hiroyuki; Yasui, Katsuaki; Kataiki, Koichi.

    1995-01-01

    The device of the present invention comprises a mechanical floating material-removing means for removing floating materials in discharged water, an ultrafiltration device for separating processed water discharged from the removing means by membranes, a reverse osmotic filtration device for separating the permeated water and a condensing means for evaporating condensed water. Since processed water after mechanically removing floating materials is supplied to the ultrafiltration device, the load applied on the filtering membrane is reduced, to simplify the operation control as a total. In addition, since the amount of resultant condensed water is reduced, and the devolumed condensed water is condensed and dried, the condensing device is made compact and the amount of resultant wastes is reduced. (T.M.)

  7. The Comparison of Layout Arrangements for the Material Flow Ordering Planning in Production Systems through Simulation Analysis

    Directory of Open Access Journals (Sweden)

    Mehmet AKSARAYLI

    2009-02-01

    Full Text Available Enterprises have to make suitable location planning to decrease their product cost and to increase their productivity in our time. The aim of this study is to compare the basic layout types used in arranging inside facility layout with each other by using simulation. Especially in this study, material handling times between machines and ratio of these times in total times were interested. First of all a new production system is designed to the basic layout types used in arranging inside facility layout. And then in the designed production system, machines are arranged for each machine layout types. Machine layout types are transferred to PROMODEL simulation software. Then with the results of analysis, material handling times of different machine layout types and ratio of material handling times in total production time were compared and the results obtained from this analysis were given after commented on this study.

  8. How do low/high height and weight variation affect upper limb movements during manual material handling of industrial boxes? Como a variação de altura e massa da carga afetam os movimentos do membro superior durante o manuseio de caixas industriais?

    Directory of Open Access Journals (Sweden)

    Ana B. Oliveira

    2011-12-01

    Full Text Available OBJECTIVES: To evaluate the effect of surface height and load weight on upper limb movements and electromyographic (EMG recordings during manual handling performed by both experienced and inexperienced lifter subjects. METHODS: Sixteen experienced and sixteen inexperienced lifters handled a box (both 7 and 15 kg from an intermediate height (waist level to either a high or low surface. Electromyography and video images were recorded during the tasks. The 10th, 50th and 90th percentiles were calculated for the deltoid and biceps muscles, shoulder flexion, shoulder abduction, and elbow flexion movements. Groups, right/left sides, weights and heights were compared. There were no differences between either groups or sides. RESULTS: Weight and height variations affected EMG and posture, although weight had more impact on EMG. Shoulder abduction and flexion movements higher than 60º occurred, particularly for the higher surface. Shoulder flexion was also higher when the box was moved to the low height. This study provides new evidence as shoulder postures during boxes handling on low surfaces had not previously been evaluated. CONCLUSIONS: The high demand of upper limb in manual material handling tasks is clear, particularly for the shoulder. This knowledge can be used by physical therapists to plan better rehabilitation programs for manual material handling-related disorders, particularly focusing on return to work.OBJETIVOS: Avaliar o efeito da altura de superfície e massa da carga nos movimentos e na atividade eletromiográfica (EMG dos membros superiores durante o manuseio de carga realizado por sujeitos experientes e inexperientes. MÉTODOS: Dezesseis sujeitos experientes e 16 inexperientes manusearam uma caixa (7 e 15 kg de uma superfície com altura intermediária para uma superfície alta e/ou baixa. Durante as tarefas, foram registradas imagens de vídeo e EMG. Os dados foram processados para obtenção dos percentis 10, 50 e 90 referentes

  9. Second-order particle-in-cell (PIC) computational method in the one-dimensional variable Eulerian mesh system

    International Nuclear Information System (INIS)

    Pyun, J.J.

    1981-01-01

    As part of an effort to incorporate the variable Eulerian mesh into the second-order PIC computational method, a truncation error analysis was performed to calculate the second-order error terms for the variable Eulerian mesh system. The results that the maximum mesh size increment/decrement is limited to be α(Δr/sub i/) 2 where Δr/sub i/ is a non-dimensional mesh size of the ith cell, and α is a constant of order one. The numerical solutions of Burgers' equation by the second-order PIC method in the variable Eulerian mesh system wer compared with its exact solution. It was found that the second-order accuracy in the PIC method was maintained under the above condition. Additional problems were analyzed using the second-order PIC methods in both variable and uniform Eulerian mesh systems. The results indicate that the second-order PIC method in the variable Eulerian mesh system can provide substantial computational time saving with no loss in accuracy

  10. An improved out-cell to in-cell rapid transfer system at the HFEF-south

    International Nuclear Information System (INIS)

    Bacca, J.P.; Sherman, E.K.

    1990-01-01

    The Argonne National Laboratory (ANL) Hot Fuel Examination Facility-South (HFEF-S), located at the ANL-West site of the Idaho National Engineering Laboratory, is currently undergoing extensive refurbishment and modifications in preparation for its use, beginning in 1991, in demonstrating remote recycling of fast reactor, metal-alloy fuel as part of the US Department of Energy liquid-metal reactor, Integral Fast Reactor (IFR) program. Included in these improvements to HFEF-S is a new, small-item, rapid transfer system (RTS). When installed, this system will enable the rapid transfer of small items from the hot-cell exterior into the argon cell (argon-gas atmosphere) of the facility without necessitating the use of time-consuming and laborious procedures. The new RTS will also provide another important function associated with HFEF-S hot-cell operation in the IFR Fuel Recycle Program; namely, the rapid insertion of clean, radioactive contamination-measuring smear paper specimens into the hot cells for area surveys, and the expedited removal of these contaminated (including alpha as well as beta/gamma contamination) smears from the argon cell for transfer to an adjacent health physics field laboratory in the facility for nuclear contamination/radiation counting

  11. Large-timestep techniques for particle-in-cell simulation of systems with applied fields that vary rapidly in space

    International Nuclear Information System (INIS)

    Friedman, A.; Grote, D.P.

    1996-10-01

    Under conditions which arise commonly in space-charge-dominated beam applications, the applied focusing, bending, and accelerating fields vary rapidly with axial position, while the self-fields (which are, on average, comparable in strength to the applied fields) vary smoothly. In such cases it is desirable to employ timesteps which advance the particles over distances greater than the characteristic scales over which the applied fields vary. Several related concepts are potentially applicable: sub-cycling of the particle advance relative to the field solution, a higher-order time-advance algorithm, force-averaging by integration along approximate orbits, and orbit-averaging. We report on our investigations into the utility of such techniques for systems typical of those encountered in accelerator studies for heavy-ion beam-driven inertial fusion

  12. Applications of the systems theory to the designing of the sand preparation sub-system in foundry plants

    Directory of Open Access Journals (Sweden)

    R. Wrona

    2009-07-01

    Full Text Available This study provides the basic principles for designing the functional structure of manufacturing systems and their components. The analysis of functional values is applied to create the technological and manufacturing model underlying the design of the foundry equipment with machine units and materials handling systems. Quoted examples illustrate the approved procedure to be applied to control the sand preparation process in a foundry.

  13. A novel in vitro method for detecting undifferentiated human pluripotent stem cells as impurities in cell therapy products using a highly efficient culture system.

    Directory of Open Access Journals (Sweden)

    Keiko Tano

    Full Text Available Innovative applications of cell therapy products (CTPs derived from human pluripotent stem cells (hPSCs in regenerative medicine are currently being developed. The presence of residual undifferentiated hPSCs in CTPs is a quality concern associated with tumorigencity. However, no simple in vitro method for direct detection of undifferentiated hPSCs that contaminate CTPs has been developed. Here, we show a novel approach for direct and sensitive detection of a trace amount of undifferentiated human induced pluripotent stem cells (hiPSCs using a highly efficient amplification method in combination with laminin-521 and Essential 8 medium. Essential 8 medium better facilitated the growth of hiPSCs dissociated into single cells on laminin-521 than in mTeSR1 medium. hiPSCs cultured on laminin-521 in Essential 8 medium were maintained in an undifferentiated state and they maintained the ability to differentiate into various cell types. Essential 8 medium allowed robust hiPSC proliferation plated on laminin-521 at low cell density, whereas mTeSR1 did not enhance the cell growth. The highly efficient culture system using laminin-521 and Essential 8 medium detected hiPSCs spiked into primary human mesenchymal stem cells (hMSCs or human neurons at the ratio of 0.001%-0.01% as formed colonies. Moreover, this assay method was demonstrated to detect residual undifferentiated hiPSCs in cell preparations during the process of hMSC differentiation from hiPSCs. These results indicate that our highly efficient amplification system using a combination of laminin-521 and Essential 8 medium is able to detect a trace amount of undifferentiated hPSCs contained as impurities in CTPs and would contribute to quality assessment of hPSC-derived CTPs during the manufacturing process.

  14. ACTIVITY-BASED COSTING DAN SIMULATED ANNEALING UNTUK PENCARIAN RUTE PADA FLEXIBLE MANUFACTURING SYSTEMS

    OpenAIRE

    Gregorius Satia Budhi

    2003-01-01

    Flexible Manufacturing System (FMS) is a manufacturing system that is formed from several Numerical Controlled Machines combine with material handling system, so that different jobs can be worked by different machines sequences. FMS combine the high productivity and flexibility of Transfer Line and Job Shop manufacturing system. In this reasearch, Activity-Based Costing(ABC) approach was used as the weight to search the operation route in the proper machine, so that the total production cost ...

  15. Transfer tunnel transporter system for the Fuels and Materials Examination Facility

    International Nuclear Information System (INIS)

    Petty, J.A.; Miller, S.C.; Richards, J.T.

    1981-01-01

    The detail design is complete and fabrication is approximately 75% complete on the Transfer Tunnel Transporter System. This system provides material handling capability for large, bulky equipment between two hot cells in a new Breeder Reactor Program support facility, the Fuels and Materials Examination Facility. One hot cell has an air atmosphere, the other a high purity inert gas atmosphere which must be maintained during transfer operations. System design features, operational capabilities and remote recovery provisions are described

  16. Material control system simulator program reference manual

    Energy Technology Data Exchange (ETDEWEB)

    Hollstien, R.B.

    1978-01-24

    A description is presented of a Material Control System Simulator (MCSS) program for determination of material accounting uncertainty and system response to particular adversary action sequences that constitute plausible material diversion attempts. The program is intended for use in situations where randomness, uncertainty, or interaction of adversary actions and material control system components make it difficult to assess safeguards effectiveness against particular material diversion attempts. Although MCSS may be used independently in the design or analysis of material handling and processing systems, it has been tailored toward the determination of material accountability and the response of material control systems to adversary action sequences.

  17. Material control system simulator program reference manual

    International Nuclear Information System (INIS)

    Hollstien, R.B.

    1978-01-01

    A description is presented of a Material Control System Simulator (MCSS) program for determination of material accounting uncertainty and system response to particular adversary action sequences that constitute plausible material diversion attempts. The program is intended for use in situations where randomness, uncertainty, or interaction of adversary actions and material control system components make it difficult to assess safeguards effectiveness against particular material diversion attempts. Although MCSS may be used independently in the design or analysis of material handling and processing systems, it has been tailored toward the determination of material accountability and the response of material control systems to adversary action sequences

  18. Principles and practices of lean production applied in a metal structures production system

    OpenAIRE

    Carvalho, Rogério; Alves, Anabela Carvalho; Lopes, Isabel da Silva

    2011-01-01

    This paper presents a work undertaken in a metal structures production system in a company producing several assorted products for the civil construction. The work aim was to improve the production process, solving several productive problems encountered in the production system, such as: deliveries delays, long lead times, too many material handling, high stocks, errors and defects in metal structures assembly and production, and unnecessary motions. The identified problems were analyzed and...

  19. A Clonal Selection Algorithm for Minimizing Distance Travel and Back Tracking of Automatic Guided Vehicles in Flexible Manufacturing System

    Science.gov (United States)

    Chawla, Viveak Kumar; Chanda, Arindam Kumar; Angra, Surjit

    2018-03-01

    The flexible manufacturing system (FMS) constitute of several programmable production work centers, material handling systems (MHSs), assembly stations and automatic storage and retrieval systems. In FMS, the automatic guided vehicles (AGVs) play a vital role in material handling operations and enhance the performance of the FMS in its overall operations. To achieve low makespan and high throughput yield in the FMS operations, it is highly imperative to integrate the production work centers schedules with the AGVs schedules. The Production schedule for work centers is generated by application of the Giffler and Thompson algorithm under four kind of priority hybrid dispatching rules. Then the clonal selection algorithm (CSA) is applied for the simultaneous scheduling to reduce backtracking as well as distance travel of AGVs within the FMS facility. The proposed procedure is computationally tested on the benchmark FMS configuration from the literature and findings from the investigations clearly indicates that the CSA yields best results in comparison of other applied methods from the literature.

  20. Applications of radio frequency identification systems in underground mining

    Energy Technology Data Exchange (ETDEWEB)

    Knights, P F; Kairouz, J; Daneshmend, L K; Pathak, J [McGill University, Montreal, PQ (Canada). Canadian Centre for Automation and Robotics in Mining

    1994-12-31

    The paper describes the application of Radio Frequency Identification (RFID) systems in underground hardrock mines. The operating principles and some of the applications of RDIF systems are described. The system operates by the exchange of information between transponder tags and an antenna and controller device. The suitability of RFID systems for process control, inventory control, materials handling, control of access, security, and transportation in underground coal and hardrock mines is discussed. An ore tonnage tracking system is under development that uses RDIF transponder tags to locate vehicles in an underground mine. 6 refs., 4 figs.

  1. Computer simulation in cell radiobiology

    International Nuclear Information System (INIS)

    Yakovlev, A.Y.; Zorin, A.V.

    1988-01-01

    This research monograph demonstrates the possible ways of using stochastic simulation for exploring cell kinetics, emphasizing the effects of cell radiobiology. In vitro kinetics of normal and irradiated cells is the main subject, but some approaches to the simulation of controlled cell systems are considered as well: the epithelium of the small intestine in mice taken as a case in point. Of particular interest is the evaluation of simulation modelling as a tool for gaining insight into biological processes and hence the new inferences from concrete experimental data, concerning regularities in cell population response to irradiation. The book is intended to stimulate interest among computer science specialists in developing new, more efficient means for the simulation of cell systems and to help radiobiologists in interpreting the experimental data

  2. Individual nuclear fuel rod weighing system

    International Nuclear Information System (INIS)

    Fogg, J. L.; Howell, C. A.; Smith, J. H.; Vining, G. E.

    1985-01-01

    An individual nuclear fuel rod weighing system for rods carried on a tray which moves along a materials handling conveyor. At a first tray position on the conveyor, a lifting device raises the rods off the tray and places them on an overhead ramp. A loading mechanism conveys the rods singly from the overhead ramp onto an overhead scale for individual weighing. When the tray is at a second position on the conveyor, a transfer apparatus transports each weighed rod from the scale back onto the tray

  3. Individual nuclear fuel rod weighing system

    International Nuclear Information System (INIS)

    Fogg, J.L.; Smith, J.H.; Vining, G.E.; Howell, C.A.

    1985-01-01

    An individual nuclear fuel rod weighing system for rods carried on a tray which moves along a materials handling conveyor is discussed. At a first tray position on the conveyor, a lifting device raises the rods off the tray and places them on an overhead ramp. A loading mechanism conveys the rods singly from the overhead ramp onto an overhead scale for individual weighing. When the tray is at a second position on the conveyor, a transfer apparatus transports each weighed rod from the scale back onto the tray

  4. Cell and Tissue Organization in the Circulatory and Ventilatory Systems Volume 1 Signaling in Cell Organization, Fate, and Activity, Part A Cell Structure and Environment

    CERN Document Server

    Thiriet, Marc

    2011-01-01

    The volumes in this authoritative series present a multidisciplinary approach to modeling and simulation of flows in the cardiovascular and ventilatory systems, especially multiscale modeling and coupled simulations. The cardiovascular and respiratory systems are tightly coupled, as their primary function is to supply oxygen to and remove carbon dioxide from the body's cells. Because physiological conduits have deformable and reactive walls, macroscopic flow behavior and prediction must be coupled to nano- and microscopic events in a corrector scheme of regulated mechanisms. Therefore, investigation of flows of blood and air in physiological conduits requires an understanding of the biology, chemistry, and physics of these systems together with the mathematical tools to describe their functioning.  The present volume is devoted to cellular events that allow adaptation to environmental conditions, particularly mechanotransduction. It begins with cell organization and a survey of cell types in the vasculatur...

  5. On the velocity space discretization for the Vlasov-Poisson system: comparison between implicit Hermite spectral and Particle-in-Cell methods

    NARCIS (Netherlands)

    E. Camporeale (Enrico); G.L. Delzanno; B.K. Bergen; J.D. Moulton

    2016-01-01

    htmlabstractWe describe a spectral method for the numerical solution of the Vlasov–Poisson system where the velocity space is decomposed by means of an Hermite basis, and the configuration space is discretized via a Fourier decomposition. The novelty of our approach is an implicit time

  6. Automated packing systems: review of industrial implementations

    Science.gov (United States)

    Whelan, Paul F.; Batchelor, Bruce G.

    1993-08-01

    A rich theoretical background to the problems that occur in the automation of material handling can be found in operations research, production engineering, systems engineering and automation, more specifically machine vision, literature. This work has contributed towards the design of intelligent handling systems. This paper will review the application of these automated material handling and packing techniques to industrial problems. The discussion will also highlight the systems integration issues involved in these applications. An outline of one such industrial application, the automated placement of shape templates on to leather hides, is also discussed. The purpose of this system is to arrange shape templates on a leather hide in an efficient manner, so as to minimize the leather waste, before they are automatically cut from the hide. These pieces are used in the furniture and car manufacturing industries for the upholstery of high quality leather chairs and car seats. Currently this type of operation is semi-automated. The paper will outline the problems involved in the full automation of such a procedure.

  7. Analysis and Design of the Logistics System for Rope Manufacturing Plant

    Directory of Open Access Journals (Sweden)

    Sun Xue

    2017-01-01

    Full Text Available In order to promote logistics system for manufacturing plant, this paper proposed a new design for the logistics system of a rope manufacturing plant. Through the analysis in the aspects of workshop facility layout, material handling and inventory management, the original logistics system of the plant is optimized. According to the comparison of the simulation results between original and optimized design, the optimized model has the higher productive efficiency. This can provide the references for the other manufacturing plant in analysis and design of the logistics system to improve plant efficiency.

  8. Robotics in a controlled, ecological life support system

    Science.gov (United States)

    Miles, Gaines E.; Krom, Kimberly J.

    1993-01-01

    Controlled, Ecological Life Support Systems (CELSS) that utilize plants to provide food, water and oxygen could consume considerable amounts of labor unless crop production, recovery and processing are automated. Robotic manipulators equipped with special end-effectors and programmed to perform the sensing and materials handling tasks would minimize the amount of astronaut labor required. The Human Rated Test Facility (HRTF) planned for Johnson Space Center could discover and demonstrate techniques of crop production which can be reliably integrated with machinery to minimize labor requirements. Before the physical components (shelves, lighting fixtures, etc.) can be selected, a systems analysis must be performed to determine which alternative processes should be followed and how the materials handling tasks should be automated. Given that the current procedures used to grow crops in a CELSS may not be the best methods to automate, then what are the alternatives? How may plants be grown, harvested, processed for food, and the inedible components recycled? What commercial technologies current exist? What research efforts are underway to develop new technologies which might satisfy the need for automation in a CELSS? The answers to these questions should prove enlightening and provide some of the information necessary to perform the systems analysis. The planting, culturing, gathering, threshing and separation, food processing, and recovery of inedible portions of wheat were studied. The basic biological and materials handling processes of each task are defined and discussed. Current practices at Johnson Space Center and other NASA centers are described and compared to common production practices in the plant production industry. Technologies currently being researched which might be applicable are identified and illustrated. Finally, based on this knowledge, several scenarios are proposed for automating the tasks for wheat.

  9. Standard handbook of chains chains for power transmission and material handling

    CERN Document Server

    2005-01-01

    A BRIEF HISTORY OF THE DEVELOPMENT OF CHAINEarly DevelopmentsCog ChainCast Detachable ChainCast Pintle ChainPrecision Roller ChainEngineering Steel ChainSilent ChainFlat-Top ChainTerminologyA CHAIN OVERVIEW: USES AND ADVANTAGESGeneralTypes of ChainScope of Chains CoveredStyles and Forms of ChainsStraight and Offset Link ChainsChains With and Without RollersUses of ChainStandard Chains and Their UsesThe Advantages of Chains in ApplicationsAdvantages of Roller Chains in DrivesAdvantages of Silent Chain Drives

  10. Pneubotics - Membrane-Based Robotics for Remote Material Handling, Phase II

    Data.gov (United States)

    National Aeronautics and Space Administration — We have invented a new class of robotics, called `Pneubotics', that rival current manipulators in payload and reach at 1/10th the weight. Our technology leverages...

  11. A study on the corrosion test of equipment material handling hot molten salt

    International Nuclear Information System (INIS)

    Ro, Seung Gy; Jeong, M.S.; Hong, S.S.; Cho, S.H.; Shin, Y.J.; Park, H.S.; Zhang, J.S.

    1999-02-01

    On this technical report, corrosion behavior of austenitic stainless steels of SUS 316L and SUS 304L in molten salt of LiCl-Li 2 O has been investigated in the temperature range of 650 - 850 dg C. Corrosion products of SUS 316L in molten salt consisted of two layers, an outer layer of LiCrO 2 and inner layer of Cr 2 O 3 .The corrosion layer was uniform in molten salt of LiCl, but the intergranular corrosion occurred in addition to the uniform corrosion in mixed molten salt of LiCl-Li 2 O. The corrosion rate increased slowly with the increase of temperature up to 750 dg C, but above 750 dg C rapid increase in corrosion rate observed. SUS 316L stainless steel showed slower corrosion rate and higher activation energy for corrosion than SUS 304L, exhibiting higher corrosion resistance in the molten salt. In heat-resistant alloy, dense protective oxide scale of LiCrO 2 was formed in molten salt of LiCl. Whereas in mixed molten salt of LiCl-Li 2 O, porous non-protective scale of Li(Cr, Ni, Fe)O 2 was formed. (Author). 44 refs., 4 tabs., 16 figs

  12. Application work risk of manual material handling operators using different lifting methods

    Science.gov (United States)

    Anizar; Matondang, AR; Sibarani, JA

    2018-02-01

    This study observed the activity of lifting and moving crates containing bottles at a pallet station in a carbonated drink factory. The activity of moving crates, each weighing 15 kg, is performed by four operators using different methods. An operator manually moves 250 crates daily and often takes rest during working. This study aims to find the most efficient method, that is using the least energy, to move crates. The workload is assessed using the method of Cardiovascular Strain Load (CVL) and energy expenditure. Operators’ arterial pulse is measured for 1 minute right before they start working and after they finish working; this is done to obtain work arterial pulse and rest arterial pulse. The way operators lift crates is analyzed using biomechanics. It is found that fatigue is experienced by all operators, with the exception of the 3rd operator who has a %CVL number below 30%. He positions the pallet to be parallel to the conveyor, and then stand in between during the lifting process. He only rotates his body to move the crate from the conveyor onto the pallet, requiring only little energy. This is one of the reasons why the %CVL number is lower than the other operators.

  13. Pneubotics - Membrane-Based Robotics for Remote Material Handling, Phase I

    Data.gov (United States)

    National Aeronautics and Space Administration — We have invented a new class of robotics, called `Pneubotics', that rival current manipulators in payload and reach at 1/10th the weight. Our technology leverages...

  14. CAD system applications to the nuclear fuel reprocessing facilities

    International Nuclear Information System (INIS)

    Morita, Eiji; Matsumoto, Tadakuni; Shikakura, Sakae; Furuya, Kousei; Sakurai, Shin-ichi.

    1994-01-01

    Effective supporting techniques of design, operation, and maintenance of the reprocessing facility have been developed using the Intergraph CAD system. Two and three dimensional views of the process cells were utilized to rationalize the equipment layout and material handling flows, and to check the piping interference. Interferences of the remote maintenance equipment with the process equipments were also evaluated by the pictures on the CAD display. The newest virtual reality technology will help our future development of the more natural simulation for the remote maintenance operator training. (author)

  15. A system of UAV application in indoor environment

    DEFF Research Database (Denmark)

    Khosiawan, Yohanes; Nielsen, Izabela Ewa

    2016-01-01

    In recent years, there has been an increased demand in the use of multiple unmanned aerial vehicles (UAVs) in indoor environments such as material handling task in a manufacturing environment and plant/environment monitoring task in a greenhouse. However, there is a lack of work reported on this ......In recent years, there has been an increased demand in the use of multiple unmanned aerial vehicles (UAVs) in indoor environments such as material handling task in a manufacturing environment and plant/environment monitoring task in a greenhouse. However, there is a lack of work reported...... on this topic. This paper presents a detailed study on several UAV systems and UAV scheduling systems. It is followed by a proposed system of UAV application in indoor environment, which comprises components of UAV system addressed in detail; focused on scheduler as the heart of operations. Consequently, system...... architecture of UAV scheduling system is presented and the framework of scheduler component is included. Scheduler component works in a phased manner to provide a systematic abstraction and achieve an efficient computation time. This study serves as a reference guide for UAV application in indoor environment....

  16. Towards systems thinking in cell biology education

    NARCIS (Netherlands)

    Verhoeff, Roald Pieter

    2003-01-01

    Students are taught a large variety of life structures and processes at the cellular level. The concepts used to describe them are mainly drawn from the sub-cellular level, but this knowledge seems to be fragmentary if its integration at the cellular and organismic level remains undone. As a

  17. Adaptive and energy efficient SMA-based handling systems

    Science.gov (United States)

    Motzki, P.; Kunze, J.; Holz, B.; York, A.; Seelecke, S.

    2015-04-01

    Shape Memory Alloys (SMA's) are known as actuators with very high energy density. This fact allows for the construction of very light weight and energy-efficient systems. In the field of material handling and automated assembly process, the avoidance of big moments of inertia in robots and kinematic units is essential. High inertial forces require bigger and stronger robot actuators and thus higher energy consumption and costs. For material handling in assembly processes, many different individual grippers for various work piece geometries are used. If one robot has to handle different work pieces, the gripper has to be exchanged and the assembly process is interrupted, which results in higher costs. In this paper, the advantages of using high energy density Shape Memory Alloy actuators in applications of material-handling and gripping-technology are explored. In particular, light-weight SMA actuated prototypes of an adaptive end-effector and a vacuum-gripper are constructed via rapid-prototyping and evaluated. The adaptive end-effector can change its configuration according to the work piece geometry and allows the handling of multiple different shaped objects without exchanging gripper tooling. SMA wires are used to move four independent arms, each arm adds one degree of freedom to the kinematic unit. At the tips of these end-effector arms, SMA-activated suction cups can be installed. The suction cup prototypes are developed separately. The flexible membranes of these suction cups are pulled up by SMA wires and thus a vacuum is created between the membrane and the work piece surface. The self-sensing ability of the SMA wires are used in both prototypes for monitoring their actuation.

  18. Medium and high energy electron beam processing system

    Energy Technology Data Exchange (ETDEWEB)

    Kashiwagi, Masayuki [Nissin-High Voltage Co., Ltd., Kyoto (Japan)

    2003-02-01

    Electron Beam Processing System (EPS) is a useful and powerful tool for industrial irradiation process. The specification of EPS is decided by consideration to irradiate what material with how thick and wide, how much dose, how to handle, in what atmosphere. In designing an EPS, it is necessary to consider safety measure such as x-ray shielding, ozone control and interlock system. The initial costs to install typical EPS are estimated for acceleration voltages from 500 kV to 5 MV, including following items; those are electron beam machine, x-ray shielding, auxiliary equipment, material handling, survey for installation, ozone exhaust duct, cooling water system, wiring and piping. These prices are reference only because the price should be changed for each case. The price of x-ray shielding should be changed by construction cost. Auxiliary equipment includes window, cooling blower, ozone exhaust blower and SF6 gas handling equipment. In installation work at site, actual workers of 3 - 4 persons for 2 months are necessary. Material handling system is considered only rolls provided in the shielding room as reference. In addition to the initial installation, operators and workers may be required to wear a personal radiation monitor. An x-ray monitor of suitable design should be installed outside the shield room to monitor x-ray level in the working area. (Y. Tanaka)

  19. New handling systems as technical support for the working process. Part 6. Feeding devices

    Energy Technology Data Exchange (ETDEWEB)

    Becher, H; Burkhardt, R; Drexel, P; Graf, B; Krreis, W

    1982-03-01

    Social, technical and economic reasons require an enhanced application of handling systems such as industrial robots. Quality and efficiency of an industrial robot depends greatly on feeding devices, and the ARGE-HHS within its project new handling systems as a technical aid in the working process intends to analyze all feeding devices that are likely to be most suitable for advanced applications. Forty one feeding devices were developed, known devices were modified, adapted to different applications, and tested. A variety of feeding devices for most applications in the field of material handling is reported.

  20. Re-Engineering Casting Production Systems - Final Report - 03/02/1998 - 03/01/2001; FINAL

    International Nuclear Information System (INIS)

    Peters, Frank; Van Voorhis, Timothy

    2001-01-01

    The goal of this three-year project was to improve the production systems in use by steel foundries in the United States. Improvements in the production systems result in less rework, less scrap, and less material handling, all of which would significantly reduce the energy demands of the process. Furthermore, these improvements would allow the companies to be more competitive, more responsive to customers' needs, deliver products with less lead time and require less capital. The ultimate result is a stronger domestic steel casting industry, which uses less energy. A major portion of this research involved the deployment of student researchers at steel foundries, to study their production systems and collect data

  1. Ricin Trafficking in Cells

    Directory of Open Access Journals (Sweden)

    Robert A. Spooner

    2015-01-01

    Full Text Available The heterodimeric plant toxin ricin binds exposed galactosyls at the cell surface of target mammalian cells, and, following endocytosis, is transported in vesicular carriers to the endoplasmic reticulum (ER. Subsequently, the cell-binding B chain (RTB and the catalytic A chain (RTA are separated reductively, RTA embeds in the ER membrane and then retrotranslocates (or dislocates across this membrane. The protein conducting channels used by RTA are usually regarded as part of the ER-associated protein degradation system (ERAD that removes misfolded proteins from the ER for destruction by the cytosolic proteasomes. However, unlike ERAD substrates, cytosolic RTA avoids destruction and folds into a catalytic conformation that inactivates its target ribosomes. Protein synthesis ceases, and subsequently the cells die apoptotically. This raises questions about how this protein avoids the pathways that are normally sanctioned for ER-dislocating substrates. In this review we focus on the molecular events that occur with non-tagged ricin and its isolated subunits at the ER–cytosol interface. This focus reveals that intra-membrane interactions of RTA may control its fate, an area that warrants further investigation.

  2. Order Picking Optimization in Carousels Storage System

    Directory of Open Access Journals (Sweden)

    Xiong-zhi Wang

    2013-01-01

    Full Text Available This paper addresses the order picking problem in a material handling system consisting of multiple carousels and one picker. Carousels are rotatable closed-loop storage systems for small items, where items are stored in bins along the loop. An order at carousels consists of n different items stored there. The objective is to find an optimal picking sequence to minimizing the total order picking time. After proving the problem to be strongly NP-hard and deriving two characteristics, we develop a dynamic programming algorithm (DPA for a special case (two-carousel storage system and an improved nearest items heuristics (INIH for the general problem. Experimental results verify that the solutions are quickly and steadily achieved and show their better performance.

  3. Non-equilibrium thermodynamics in cells.

    Science.gov (United States)

    Jülicher, Frank; Grill, Stephan W; Salbreux, Guillaume

    2018-03-15

    We review the general hydrodynamic theory of active soft materials that is motivated in partic- ular by biological matter. We present basic concepts of irreversible thermodynamics of spatially extended multicomponent active systems. Starting from the rate of entropy production, we iden- tify conjugate thermodynamic fluxes and forces and present generic constitutive equations of polar active fluids and active gels. We also discuss angular momentum conservation which plays a role in the the physics of active chiral gels. The irreversible thermodynamics of active gels provides a general framework to discuss the physics that underlies a wide variety of biological processes in cells and in multicellular tissues. © 2018 IOP Publishing Ltd.

  4. Chansporter complexes in cell signaling.

    Science.gov (United States)

    Abbott, Geoffrey W

    2017-09-01

    Ion channels facilitate diffusion of ions across cell membranes for such diverse purposes as neuronal signaling, muscular contraction, and fluid homeostasis. Solute transporters often utilize ionic gradients to move aqueous solutes up their concentration gradient, also fulfilling a wide variety of tasks. Recently, an increasing number of ion channel-transporter ('chansporter') complexes have been discovered. Chansporter complex formation may overcome what could otherwise be considerable spatial barriers to rapid signal integration and feedback between channels and transporters, the ions and other substrates they transport, and environmental factors to which they must respond. Here, current knowledge in this field is summarized, covering both heterologous expression structure/function findings and potential mechanisms by which chansporter complexes fulfill contrasting roles in cell signaling in vivo. © 2017 Federation of European Biochemical Societies.

  5. Analysis of reconfigurable assembly system framing systems in automotive industry

    Directory of Open Access Journals (Sweden)

    Md Zain Mohamad Zamri

    2017-01-01

    Full Text Available Current trend in automotive industry shows increasing demand for multiple models with lean production. Prior to that, automotive manufacturing systems evolved from mass production to flexible automation. Material handling systems and equipment in a single assembly line with multiple models require high investment but with low throughput thus making production cost relatively high. Current assembly process of side structure and undercarriage with downtime occurrence during assembly process affecting production performance (quality, cost and delivery. Manufacturing facilities should allow more flexibility and increase intelligence evolving toward novel reconfigurable assembly systems (RAS. RAS is envisaged capable of increasing factor flexibility and responsiveness by incorporating assembly jig, robot and framing, which could be next generation of world class automotive assembly systems. This project research proposes a new methodology of framework reconfigurable assembly systems principles in automotive framing systems i.e. enhance assembly process between side structure assembly and undercarriage assembly which a new RAS is capable to reconfigure the assembly processes of multiple model on a single assembly line. Simulation software (Witness will be used to simulate and validate current and proposed assembly process. RAS is expected to be a solution for rapid change in structure and for a responsively adjustable production capacity. Quality, cost and delivery are production key parameters that can be achieved by implementing RAS.

  6. New Concepts and Theories For Intelligent Control of Cellular Manufacturing Systems

    DEFF Research Database (Denmark)

    Langer, Gilad

    1996-01-01

    This paper will present some new theories such as biological manufacturing system, the fractal factory theory, holonic manufacturing systems, agile manufacturing, object orientation, multi-agent theory, artificial intelligence, and artificial life in the context of manufacturing systems....... The paper tries to encapsulate the main area of my Ph.D. thesis research which will evolve around the idea of integrating intelligent elements into the control systems of the manufacturing systems. Furthermore it intends to show how the curriculum and discussions of the IPS Ph.D. course will and have...... contributed to my research. The research will concentrate on integration of manufacturing units by use of intelligent control mechanisms, information technology and the material handling as the key integrators....

  7. A robotic system for automation of logistics functions on the Space Station

    Science.gov (United States)

    Martin, J. C.; Purves, R. B.; Hosier, R. N.; Krein, B. A.

    1988-01-01

    Spacecraft inventory management is currently performed by the crew and as systems become more complex, increased crew time will be required to perform routine logistics activities. If future spacecraft are to function effectively as research labs and production facilities, the efficient use of crew time as a limited resource for performing mission functions must be employed. The use of automation and robotics technology, such as automated warehouse and materials handling functions, can free the crew from many logistics tasks and provide more efficient use of crew time. Design criteria for a Space Station Automated Logistics Inventory Management System is focused on through the design and demonstration of a mobile two armed terrestrial robot. The system functionally represents a 0 gravity automated inventory management system and the problems associated with operating in such an environment. Features of the system include automated storage and retrieval, item recognition, two armed robotic manipulation, and software control of all inventory item transitions and queries.

  8. 29 CFR 1917.50 - Certification of marine terminal material handling devices (See also mandatory appendix I, of...

    Science.gov (United States)

    2010-07-01

    ... be made readily available for inspection. (3) Vertical pocket or bucket conveyors such as banana... paragraph (b)(2) of this section and transferred to a job site in another state, the current certification...

  9. Is There an Economic Case for Training Intervention in the Manual Material Handling Sector of Developing Countries?

    Science.gov (United States)

    Lahiri, Supriya; Tempesti, Tommaso; Gangopadhyay, Somnath

    2016-02-01

    To estimate cost-effectiveness ratios and net costs of a training intervention to reduce morbidity among porters who carry loads without mechanical assistance in a developing country informal sector setting. Pre- and post-intervention survey data (n = 100) were collected in a prospective study: differences in physical/mental composite scores and pain scale scores were computed. Costs and economic benefits of the intervention were monetized with a net-cost model. Significant changes in physical composite scores (2.5), mental composite scores (3.2), and pain scale scores (-1.0) led to cost-effectiveness ratios of $6.97, $5.41, and $17.91, respectively. Multivariate analysis showed that program adherence enhanced effectiveness. The net cost of the intervention was -$5979.00 due to a reduction in absenteeism. Workplace ergonomic training is cost-effective and should be implemented wherein other engineering-control interventions are precluded due to infrastructural constraints.

  10. Ergonomic interventions for improving working postures associated with manual materials handling (case study: a mineral processing plant

    Directory of Open Access Journals (Sweden)

    Fateme Dehghani

    2016-12-01

    Full Text Available Introduction: A high percentage of musculoskeletal disorders in workplaces occur due to awkward posture and non-ergonomic design of the work stations for lifting and carrying of materials. To avoid these injuries, jobs should be designed in a way that ergonomics risk factors are controlled properly. The aim of this study was to utilize ergonomics interventions to minimize ergonomics risk factors in bag packing unit in a mineral processing plant. Material and Method: This cross sectional study was carried out among 20 workers of bag packing unit. Camera recording of working postures, evaluation of medical records, interview, and REBA technique were used to identify the ergonomic risk factors. Interventions included changing the conveyor belt height and the use of spring pallets (spring table. Data were analyzed using Paired T-Test by SPSS software version 18. Result: Before implementing ergonomics intervention, a total of 75% of evaluated postures by REBA technique obtained score of 8-10 (very high risk level and 25% had score of 11-15 (very high risk level that correspond to the action level 3 and 4, respectively. Following the implementation of ergonomics interventions, a total of 90% of the analyzed postures showed action level 2 (moderate risk level and the remainder 10 percent of evaluated postures showed high risk level. Comparison of REBA technique scores before and after implementing interventions showed a significant difference (P-value < 0.05. Conclusion: Based on the findings of this study, the implementation of ergonomics interventions has remarkably decreased the required action level and it may be able to improve work-related postures.

  11. Decree 2210: by means of which technical standards and allowed proceedings for radioactive material handling are issued

    International Nuclear Information System (INIS)

    1992-01-01

    This Decree has the regulation and handling of radioactive material as object, in order to protect the health of people as well as the atmosphere. These regulations are applicable to: all natural or artificial person, public or private, that imports, manufacture, transport, store, trade, transfer or use with industrial, commercial, scientific, medical or any other aim; apparatuses capable to generate ionizing radiations whose quantum energy is superior to 5 k lo electron volts (KeV) or materials that contain radionuclides whose activities surpass the maxim exempt registration; notification and license concession. It includes: definitions; signalizing by means of basic symbol that must be included in all object, material and their mixtures, that emit ionizing radiations; control; production; import and export; trade; use; and transport of activities that involve materials and apparatuses capable to generate ionizing radiations; the category and labeled of bundles; the limits of activity for excepted bundles; the corresponding values for the different radionuclides; the limits of activity for the means of material haulage; the storage; and the handling of radioactive waste [es

  12. Validating the JobFit system functional assessment method

    Energy Technology Data Exchange (ETDEWEB)

    Jenny Legge; Robin Burgess-Limerick

    2007-05-15

    Workplace injuries are costing the Australian coal mining industry and its communities $410 Million a year. This ACARP study aims to meet those demands by developing a safe, reliable and valid pre-employment functional assessment tool. All JobFit System Pre-Employment Functional Assessments (PEFAs) consist of a musculoskeletal screen, balance test, aerobic fitness test and job-specific postural tolerances and material handling tasks. The results of each component are compared to the applicant's job demands and an overall PEFA score between 1 and 4 is given with 1 being the better score. The reliability study and validity study were conducted concurrently. The reliability study examined test-retest, intra-tester and inter-tester reliability of the JobFit System Functional Assessment Method. Overall, good to excellent reliability was found, which was sufficient to be used for comparison with injury data for determining the validity of the assessment. The overall assessment score and material handling tasks had the greatest reliability. The validity study compared the assessment results of 336 records from a Queensland underground and open cut coal mine with their injury records. A predictive relationship was found between PEFA score and the risk of a back/trunk/shoulder injury from manual handling. An association was also found between PEFA score of 1 and increased length of employment. Lower aerobic fitness test results had an inverse relationship with injury rates. The study found that underground workers, regardless of PEFA score, were more likely to have an injury when compared to other departments. No relationship was found between age and risk of injury. These results confirm the validity of the JobFit System Functional Assessment method.

  13. Stochastic processes in cell biology

    CERN Document Server

    Bressloff, Paul C

    2014-01-01

    This book develops the theory of continuous and discrete stochastic processes within the context of cell biology.  A wide range of biological topics are covered including normal and anomalous diffusion in complex cellular environments, stochastic ion channels and excitable systems, stochastic calcium signaling, molecular motors, intracellular transport, signal transduction, bacterial chemotaxis, robustness in gene networks, genetic switches and oscillators, cell polarization, polymerization, cellular length control, and branching processes. The book also provides a pedagogical introduction to the theory of stochastic process – Fokker Planck equations, stochastic differential equations, master equations and jump Markov processes, diffusion approximations and the system size expansion, first passage time problems, stochastic hybrid systems, reaction-diffusion equations, exclusion processes, WKB methods, martingales and branching processes, stochastic calculus, and numerical methods.   This text is primarily...

  14. Quality assurance procedures for the IAEA Department of Safeguards Twin Minolta Camera Surveillance System

    International Nuclear Information System (INIS)

    Geoffrion, R.R.; Bussolini, P.L.; Stark, W.A.; Ahlquist, A.J.; Sanders, K.E.; Rubinstein, G.

    1986-01-01

    The International Atomic Energy Agency (IAEA) safeguards program provides assurance to the international community that nations are complying with nuclear safeguards treaties. In one aspect of the program, the Department of Safeguards has developed a twin Minolta camera photo surveillance systems program to assure itself and the international community that material handling is accomplished according to safeguards treaty regulations. The camera systems are positioned in strategic locations in facilities such that objective evidence can be obtained for material transactions. The films are then processed, reviewed, and used to substantiate the conclusions that nuclear material has not been diverted. Procedures have been developed to document and aid in: 1) the performance of activities involved in positioning of the camera system; 2) installation of the systems; 3) review and use of the film taken from the cameras

  15. Genes involved in cell division in mycoplasmas

    Directory of Open Access Journals (Sweden)

    Frank Alarcón

    2007-01-01

    Full Text Available Bacterial cell division has been studied mainly in model systems such as Escherichia coli and Bacillus subtilis, where it is described as a complex process with the participation of a group of proteins which assemble into a multiprotein complex called the septal ring. Mycoplasmas are cell wall-less bacteria presenting a reduced genome. Thus, it was important to compare their genomes to analyze putative genes involved in cell division processes. The division and cell wall (dcw cluster, which in E. coli and B. subtilis is composed of 16 and 17 genes, respectively, is represented by only three to four genes in mycoplasmas. Even the most conserved protein, FtsZ, is not present in all mycoplasma genomes analyzed so far. A model for the FtsZ protein from Mycoplasma hyopneumoniae and Mycoplasma synoviae has been constructed. The conserved residues, essential for GTP/GDP binding, are present in FtsZ from both species. A strong conservation of hydrophobic amino acid patterns is observed, and is probably necessary for the structural stability of the protein when active. M. synoviae FtsZ presents an extended amino acid sequence at the C-terminal portion of the protein, which may participate in interactions with other still unknown proteins crucial for the cell division process.

  16. Arnold Tongues in Cell Dynamics

    Science.gov (United States)

    Jensen, Mogens

    In a recent work with Leo Kadanoff we studied the synchronization between an internal and an external frequency. One obtains a highly structured diagram with details that in essence are related to the difference between rational and irrational number. The synchronized regions appear as Arnold tongues that widen as the coupling between the frequencies increases. Such tongues have been observed in many physical systems, like in the Libchaber convective cell in the basement of the University of Chicago. In biological systems, where oscillators appear in in a broad variety, very little research on Arnold tongues has been performed. We discuss single cell oscillating dynamics triggered by an external cytokine signal. When this signal is overlaid by an oscillating variation, the two oscillators might couple leading to Arnold tongue diagram. When the tongues overlap, the cell dynamics can shift between the tongues eventually leading to a chaotic response. We quantify such switching in single cell experiments and in model systems based on Gillespie simulations. Kadanoff session.

  17. An integrated multi-criteria decision-making methodology for conveyor system selection

    Directory of Open Access Journals (Sweden)

    Pairat Jiamruangjarus

    2016-12-01

    Full Text Available Material handling equipment (MHE is important for every industry because it has an effect on the productivity of manufacturing. Conveyor systems are presently one popular type of MHE. This paper presents an integration of the analytic network process (ANP with the benefits, opportunities, costs and risk (BOCR model in order to select the best conveyor system. The proposed model established a network with four merits, six strategies criteria, and twenty six sub-criteria with four alternatives (present, roller conveyor, chain conveyor, and monorail. The ANP is to determine the relative weights of an evaluative criteria and decision alternatives. Therefore, the final ranking of the alternatives are calculated by synthesizing the score of each alternative under BOCR. The results showed that the best alternative under all five methods is the chain conveyor. These research results can be easily applied, adapted and used to improve performance of selecting the conveyer system in small and medium enterprises through large industries.

  18. Endocrine disrupting potentials of Bisphenol A, Bisphenol A dimethacrylate, 4-n-Nonyl-phenol and 4-Octylphenol assessed in cell model systems for effects on the estrogen-, androgen-, aryl hydrocarbon-receptor and aromatase activity

    DEFF Research Database (Denmark)

    Bonefeld-Jørgensen, Eva Cecilie; Long, Manhai; Hofmeister, Marlene V

    used as surfactants. We have investigated the effect in vitro of these four plasticizers in four cell culture model systems.The estrogenic potencies were analyzed using the stable ERE-luciferase transfected cell line MVLN measuring the relative estrogen receptor (ER) transactivated luciferase units......, and activity of aromatase and AhR transactivation.Acknowledgement. The authors contributed equally to this work. We thank technical assistants Anne Keblovszki and Inger Sørensen for their excellent skills in the laboratory work. The data is a part of the European Union project ENDOMET: Dysregulation...... of endogenous steroid metabolism potentially alters neuronal and reproductive system development: effects of environmental plasticizers. Program "Quality of Life and Management of Living Resources". (Contract no. QLK4-CT-2002-02637). http://endomet.bham.ac.uk                                                                                                                                                                                                                         ....

  19. A mathematical model in cellular manufacturing system considering subcontracting approach under constraints

    Directory of Open Access Journals (Sweden)

    Kamran Forghani

    2012-10-01

    Full Text Available In this paper, a new mathematical model in cellular manufacturing systems (CMSs has been presented. In order to increase the performance of manufacturing system, the production quantity of parts has been considered as a decision variable, i.e. each part can be produced and outsourced, simultaneously. This extension would be minimized the unused capacity of machines. The exceptional elements (EEs are taken into account and would be totally outsourced to the external supplier in order to remove intercellular material handling cost. The problem has been formulated as a mixed-integer programming to minimize the sum of manufacturing variable costs under budget, machines capacity and demand constraints. Also, to evaluate advantages of the model, several illustrative numerical examples have been provided to compare the performance of the proposed model with the available classical approaches in the literature.

  20. Solving a mathematical model integrating unequal-area facilities layout and part scheduling in a cellular manufacturing system by a genetic algorithm.

    Science.gov (United States)

    Ebrahimi, Ahmad; Kia, Reza; Komijan, Alireza Rashidi

    2016-01-01

    In this article, a novel integrated mixed-integer nonlinear programming model is presented for designing a cellular manufacturing system (CMS) considering machine layout and part scheduling problems simultaneously as interrelated decisions. The integrated CMS model is formulated to incorporate several design features including part due date, material handling time, operation sequence, processing time, an intra-cell layout of unequal-area facilities, and part scheduling. The objective function is to minimize makespan, tardiness penalties, and material handling costs of inter-cell and intra-cell movements. Two numerical examples are solved by the Lingo software to illustrate the results obtained by the incorporated features. In order to assess the effects and importance of integration of machine layout and part scheduling in designing a CMS, two approaches, sequentially and concurrent are investigated and the improvement resulted from a concurrent approach is revealed. Also, due to the NP-hardness of the integrated model, an efficient genetic algorithm is designed. As a consequence, computational results of this study indicate that the best solutions found by GA are better than the solutions found by B&B in much less time for both sequential and concurrent approaches. Moreover, the comparisons between the objective function values (OFVs) obtained by sequential and concurrent approaches demonstrate that the OFV improvement is averagely around 17 % by GA and 14 % by B&B.

  1. Genesis Eco Systems, Inc. soil washing process

    International Nuclear Information System (INIS)

    Cena, R.J.

    1994-01-01

    The Genesis soil washing system is an integrated system of modular design allowing for maximum material handling capabilities, with optimized use of space for site mobility. The Surfactant Activated Bio-enhanced Remediation Equipment-Generation 1 (SABRE-1, Patent Applied For) modification was developed specifically for removing petroleum byproducts from contaminated soils. Scientifically formulated surfactants, introduced by high pressure spray nozzles, displace the contaminant from the surface of the soil particles into the process solution. Once the contaminant is dispersed into the liquid fraction of the process, it is either mechanically removed, chemically oxidized, or biologically oxidized. The contaminated process water is pumped through the Genesis Biosep (Patent Applied For) filtration system where the fines portion is flocculated, and the contaminant-rich liquid portion is combined with an activated mixture of nutrients and carefully selected bacteria to decompose the hydrocarbon fraction. The treated soil and dewatered fines are transferred to a bermed stockpile where bioremediation continues during drying. The process water is reclaimed, filtered, and recycled within the system

  2. Functional imaging of microdomains in cell membranes.

    Science.gov (United States)

    Duggan, James; Jamal, Ghadir; Tilley, Mark; Davis, Ben; McKenzie, Graeme; Vere, Kelly; Somekh, Michael G; O'Shea, Paul; Harris, Helen

    2008-10-01

    The presence of microdomains or rafts within cell membranes is a topic of intense study and debate. The role of these structures in cell physiology, however, is also not yet fully understood with many outstanding problems. This problem is partly based on the small size of raft structures that presents significant problems to their in vivo study, i.e., within live cell membranes. But the structure and dynamics as well as the factors that control the assembly and disassembly of rafts are also of major interest. In this review we outline some of the problems that the study of rafts in cell membranes present as well as describing some views of what are considered the generalised functions of membrane rafts. We point to the possibility that there may be several different 'types' of membrane raft in cell membranes and consider the factors that affect raft assembly and disassembly, particularly, as some researchers suggest that the lifetimes of rafts in cell membranes may be sub-second. We attempt to review some of the methods that offer the ability to interrogate rafts directly as well as describing factors that appear to affect their functionality. The former include both near-field and far-field optical approaches as well as scanning probe techniques. Some of the advantages and disadvantages of these techniques are outlined. Finally, we describe our own views of raft functionality and properties, particularly, concerning the membrane dipole potential, and describe briefly some of the imaging strategies we have developed for their study.

  3. Isolation and Characterization of Poliovirus in Cell Culture Systems.

    Science.gov (United States)

    Thorley, Bruce R; Roberts, Jason A

    2016-01-01

    The isolation and characterization of enteroviruses by cell culture was accepted as the "gold standard" by clinical virology laboratories. Methods for the direct detection of all enteroviruses by reverse transcription polymerase chain reaction, targeting a conserved region of the genome, have largely supplanted cell culture as the principal diagnostic procedure. However, the World Health Organization's Global Polio Eradication Initiative continues to rely upon cell culture to isolate poliovirus due to the lack of a reliable sensitive genetic test for direct typing of enteroviruses from clinical specimens. Poliovirus is able to infect a wide range of mammalian cell lines, with CD155 identified as the primary human receptor for all three seroytpes, and virus replication leads to an observable cytopathic effect. Inoculation of cell lines with extracts of clinical specimens and subsequent passaging of the cells leads to an increased virus titre. Cultured isolates of poliovirus are suitable for testing by a variety of methods and remain viable for years when stored at low temperature.This chapter describes general procedures for establishing a cell bank and routine passaging of cell lines. While the sections on specimen preparation and virus isolation focus on poliovirus, the protocols are suitable for other enteroviruses.

  4. Effect of Nanoparticles on Complement System in Cell Culture Model

    National Research Council Canada - National Science Library

    Sladowski, Dariusz T

    2006-01-01

    ... Corporation San Diego California, Different sizes of nanoparticles such as silver (Ag; 151,000 nm) molybdenum (MoO3; 30 150 nm), aluminum (Al; 30 103 nm), iron oxide (Fe3O4; 30, 47 nm) and titanium dioxide...

  5. Modeling cell-in-cell structure into its biological significance

    OpenAIRE

    He, M-f; Wang, S; Wang, Y; Wang, X-n

    2013-01-01

    Although cell-in-cell structure was noted 100 years ago, the molecular mechanisms of ?entering' and the destination of cell-in-cell remain largely unclear. It takes place among the same type of cells (homotypic cell-in-cell) or different types of cells (heterotypic cell-in-cell). Cell-in-cell formation affects both effector cells and their host cells in multiple aspects, while cell-in-cell death is under more intensive investigation. Given that cell-in-cell has an important role in maintainin...

  6. RASCAL 4.l: evaluation system for the analysis of the radiological consequences

    International Nuclear Information System (INIS)

    Miguel, I. de; Gomez-Arguello, B.

    2012-01-01

    Recently the Spanish Nuclear Safety Council (CSN) has promoted the replacement of the code used in the Spanish Nuclear Power Plants to estimate potential and actual doses due to atmospheric releases. IRDAM Code (interactive Rapid Dose Assessment Model, 1983) by RASCAL Code version 4.1 (Radiological Assessment System for consequence Analysis). This code was developed by the NRC (Nuclear Regulatory Commission) to evaluate releases from nuclear power plants, spent fuel storage pools and casks, fuel cycle facilities and radioactive material handling facilities. RASCAL was officially requested to the Spanish nuclear power plants in February 1st, 2012, and it can be used for external dose assessment and to evaluate the recommended protective action for the public in a specific situation (sheltering, prophylaxis, evacuation, etc.). (Author)

  7. Designing a mathematical model for integrating dynamic cellular manufacturing into supply chain system

    Science.gov (United States)

    Aalaei, Amin; Davoudpour, Hamid

    2012-11-01

    This article presents designing a new mathematical model for integrating dynamic cellular manufacturing into supply chain system with an extensive coverage of important manufacturing features consideration of multiple plants location, multi-markets allocation, multi-period planning horizons with demand and part mix variation, machine capacity, and the main constraints are demand of markets satisfaction in each period, machine availability, machine time-capacity, worker assignment, available time of worker, production volume for each plant and the amounts allocated to each market. The aim of the proposed model is to minimize holding and outsourcing costs, inter-cell material handling cost, external transportation cost, procurement & maintenance and overhead cost of machines, setup cost, reconfiguration cost of machines installation and removal, hiring, firing and salary worker costs. Aimed to prove the potential benefits of such a design, presented an example is shown using a proposed model.

  8. Technological and mining analysis of mechanized systems used in roadways in Polish mines

    Energy Technology Data Exchange (ETDEWEB)

    Sikora, W; Giza, T; Siwiec, J [Politechnika Slaska, Gliwice (Poland). Instytut Mechanizacji Gornictwa

    1987-01-01

    Analyzes methods of mine drivage in Poland and materials handling systems. Of 1,620 km of roadways driven in 1982, 12% fell on roadways driven in coal and 88% on roadways driven in stone or stone and coal. Roadways driven in coal in most cases were situated at depths from 500 to 700 m. Roadway cross-section ranged from 12 to 18 m{sup 2}. Roadways in stone or stone and coal were driven by drilling and blasting. Loaders were used for stone handling. Roadways in coal were driven by heading machines. Advance rates of mine drivage by heading machines were 2 to 3 times higher than those by drilling and blasting with loaders for stone handling. Basic statistical data characterizing roadways and drivage methods are evaluated: roadway dimensions and depth advance rate depending on drivage methods and mining condition, types of heading machines and loaders.

  9. Shiftable conveyor systems in open-cast mines in India

    Energy Technology Data Exchange (ETDEWEB)

    Noronha, H S; Patel, J C

    1984-12-01

    The materials handling and earthmoving arrangements are described at the Neyveli lignite mine in Tamil Nadu State, 200 km from Madras, India. The equipment used consists of shiftable conveyors, transfer feeders and drive stations.

  10. Systems

    International Nuclear Information System (INIS)

    Anon.

    1980-01-01

    Papers in this session describe the concept of mined geologic disposal system and methods for ensuring that the system, when developed, will meet all technical requirements. Also presented in the session are analyses of system parameters, such as cost and nuclear criticality potential, as well as a technical analysis of a requirement that the system permit retrieval of the waste for some period of time. The final paper discusses studies under way to investigate technical alternatives or complements to the mined geologic disposal system. Titles of the presented papers are: (1) Waste Isolation System; (2) Waste Isolation Economics; (3) BWIP Technical Baseline; (4) Criticality Considerations in Geologic Disposal of High-Level Waste; (5) Retrieving Nuclear Wastes from Repository; (6) NWTS Programs for the Evaluation of Technical Alternatives or Complements to Mined Geologic Repositories - Purpose and Objectives

  11. systems

    Directory of Open Access Journals (Sweden)

    Alexander Leonessa

    2000-01-01

    Full Text Available A nonlinear robust control-system design framework predicated on a hierarchical switching controller architecture parameterized over a set of moving nominal system equilibria is developed. Specifically, using equilibria-dependent Lyapunov functions, a hierarchical nonlinear robust control strategy is developed that robustly stabilizes a given nonlinear system over a prescribed range of system uncertainty by robustly stabilizing a collection of nonlinear controlled uncertain subsystems. The robust switching nonlinear controller architecture is designed based on a generalized (lower semicontinuous Lyapunov function obtained by minimizing a potential function over a given switching set induced by the parameterized nominal system equilibria. The proposed framework robustly stabilizes a compact positively invariant set of a given nonlinear uncertain dynamical system with structured parametric uncertainty. Finally, the efficacy of the proposed approach is demonstrated on a jet engine propulsion control problem with uncertain pressure-flow map data.

  12. Visualization of particle in cell simulation

    International Nuclear Information System (INIS)

    Chen Ming; Cheng Yinhui

    2003-01-01

    This paper is trying to provide a new technique of the visualization for the Particle In Cell simulation, which takes effect by using the MATLAB external interface, so the real-time obsevation of particles came easier and more efficient. With this method, state of the particles, considered as 'particle cloud' can be found in the image produced automatically and their movement can be predicted. (authors)

  13. Mockup of an automated material transport system for remote handling

    International Nuclear Information System (INIS)

    Porter, M.L.

    1992-01-01

    An Automated Material Transport System (AMTS) was identified for transport of samples within a Material and Process Control Laboratory (MPCL). The MPCL was designed with a dry sample handling laboratory and a wet chemistry analysis laboratory. Each laboratory contained several processing gloveboxes. The function of the AMTS was to automate the handling of materials, multiple process samples, and bulky items between process stations with a minimum of operator intervention and with minimum o[ waiting periods and nonproductive activities. This paper discusses the system design features, capabilities and results of initial testing. The overall performance of the AMTS is very good. No major problems or concerns were identified. System commands are simple and logical making the system user friendly. Operating principle and design of individual components is simple. With the addition of various track modules, the system can be configured in most any configuration. The AMTS lends itself very well for integration with other automated systems or products. The AMTS is suited for applications involving light payloads which require multiple sample and material handling, lot tracking, and system integration with other products

  14. ATW system impact on high-level waste

    International Nuclear Information System (INIS)

    Arthur, E.D.

    1992-01-01

    This report discusses the Accelerator Transmutation of Waste (ATW) concept which aims at destruction of key long-lived radionuclides in high-level nuclear waste (HLW), both fission products and actinides. This focus makes it different from most other transmutation concepts which concentrate primarily on actinide burning. The ATW system uses an accelerator-driven, sub-critical assembly to create an intense thermal neutron environment for radionuclide transmutation. This feature allows rapid transmutation under low-inventory system conditions, which in turn, has a direct impact on the size of chemical separations and materials handling components of the system. Inventories in ATW are factors of eight to thirty times smaller than reactor systems of equivalent thermal power. Chemical separations systems are relatively small in scale and can be optimized to achieve high decontamination factors and minimized waste streams. The low-inventory feature also directly impacts material amounts remaining in the system at its end of life. In addition to its low-inventory operation, the accelerator-driven neutron source features of ATW are key to providing a sufficient level of neutrons to allow transmutation of long-lived fission products

  15. Ion Channels Involved in Cell Volume Regulation

    DEFF Research Database (Denmark)

    Hoffmann, Else Kay

    2011-01-01

    regulatory ion channels involved, and the mechanisms (cellular signalling pathways) that regulate these channels. Finally, I shall also briefly review current investigations in these two cell lines that focuses on how changes in cell volume can regulate cell functions such as cell migration, proliferation......This mini review outlines studies of cell volume regulation in two closely related mammalian cell lines: nonadherent Ehrlich ascites tumour cells (EATC) and adherent Ehrlich Lettre ascites (ELA) cells. Focus is on the regulatory volume decrease (RVD) that occurs after cell swelling, the volume...

  16. Design study on advanced nuclear fuel recycle system. Conceptual design study of recycle system using molten salt

    International Nuclear Information System (INIS)

    Kasai, Y.; Kakehi, I.; Moro, T.; Higashi, T.; Tobe, K.; Kawamura, F.; Yonezawa, S.; Yoshiuji, T.

    1998-10-01

    Advanced recycle system engineering group of OEC (Oarai Engineering Center) has being carried out a design study of the advanced nuclear fuel recycle system using molten salt (electro-metallurgical process). This system is aiming for improvements of fuel cycle economy and reduction of environmental burden (MA recycles, Minimum of radioactive waste disposal), and also improvement of safety and nuclear non-proliferation. This report describes results of the design study that has been continued since December 1996. (1) A design concept of the advanced nuclear fuel recycle system, that is a module type recycles system of pyrochemical reprocessing and fuel re-fabrication was studied. The module system has advantage in balance of Pu recycle where modules are constructed in coincidence with the construction plan of nuclear power plants, and also has flexibility for technology progress. A demonstration system, minimum size of the above module, was studies. This system has capacity of 10 tHM/y and is able to demonstrate recycle technology of MOX fuel, metal fuel and nitride fuel. (2) Each process of the system, which are pyrochemical electrorefining system, cathode processor, de-cladding system, waste disposal system, etc., were studied. In this study, capacity of an electrorefiner was discussed, and vitrification experiment of molten salt using lead-boric acid glass was conducted. (3) A hot cell system and material handling system of the demonstration system was studied. A robot driven by linear motor was studied for the handling system, and an arrangement plan of the cell system was made. Criticality analysis in the cell system and investigation of material accountancy system of the recycle plant were also made. This design study will be continued in coincidence with design study of reactor and fuel, aiming to establish the concept of FBR recycle system. (author)

  17. Analysis on flexible manufacturing system layout using arena simulation software

    Science.gov (United States)

    Fadzly, M. K.; Saad, Mohd Sazli; Shayfull, Z.

    2017-09-01

    Flexible manufacturing system (FMS) was defined as highly automated group technology machine cell, consisting of a group of processing stations interconnected by an automated material handling and storage system, and controlled by an integrated computer system. FMS can produce parts or products are in the mid-volume, mid-variety production range. The layout system in FMS is an important criterion to design the FMS system to produce a part or product. This facility layout of an FMS involves the positioning of cells within given boundaries, so as to minimize the total projected travel time between cells. Defining the layout includes specifying the spatial coordinates of each cell, its orientation in either a horizontal or vertical position, and the location of its load or unloads point. There are many types of FMS layout such as In-line, loop ladder and robot centered cell layout. The research is concentrating on the design and optimization FMS layout. The final conclusion can be summarized that the objective to design and optimisation of FMS layout for this study is successful because the FMS In-line layout is the best layout based on effective time and cost using ARENA simulation software.

  18. Computer-assisted Particle-in-Cell code development

    International Nuclear Information System (INIS)

    Kawata, S.; Boonmee, C.; Teramoto, T.; Drska, L.; Limpouch, J.; Liska, R.; Sinor, M.

    1997-12-01

    This report presents a new approach for an electromagnetic Particle-in-Cell (PIC) code development by a computer: in general PIC codes have a common structure, and consist of a particle pusher, a field solver, charge and current density collections, and a field interpolation. Because of the common feature, the main part of the PIC code can be mechanically developed on a computer. In this report we use the packages FIDE and GENTRAN of the REDUCE computer algebra system for discretizations of field equations and a particle equation, and for an automatic generation of Fortran codes. The approach proposed is successfully applied to the development of 1.5-dimensional PIC code. By using the generated PIC code the Weibel instability in a plasma is simulated. The obtained growth rate agrees well with the theoretical value. (author)

  19. Where to Go: Breaking the Symmetry in Cell Motility

    Science.gov (United States)

    2016-01-01

    Cell migration in the “correct” direction is pivotal for many biological processes. Although most work is devoted to its molecular mechanisms, the cell’s preference for one direction over others, thus overcoming intrinsic random motility, epitomizes a profound principle that underlies all complex systems: the choice of one axis, in structure or motion, from a uniform or symmetric set of options. Explaining directional motility by an external chemo-attractant gradient does not solve but only shifts the problem of causation: whence the gradient? A new study in PLOS Biology shows cell migration in a self-generated gradient, offering an opportunity to take a broader look at the old dualism of extrinsic instruction versus intrinsic symmetry-breaking in cell biology. PMID:27196433

  20. Electron Microscopy of Nanostructures in Cells

    DEFF Research Database (Denmark)

    Købler, Carsten

    with cells is therefore increasingly more relevant from both an engineering and a toxicological viewpoint. My work involves developing and exploring electron microscopy (EM) for imaging nanostructures in cells, for the purpose of understanding nanostructure-cell interactions in terms of their possibilities...... in science and concerns in toxicology. In the present work, EM methods for imaging nanostructure-cell interactions have been explored, and the complex interactions documented and ordered. In particular the usability of the focused ion beam scanning electron microscope (FIB-SEM) was explored. Using EM...... in literature. Furthermore, EM proved valuable as it revealed an unnoticed CNT effect. FIB-SEM helped establish that the effect was linked to eosionophilic crystalline pneumonia (ECP)....

  1. Development of a telerobotic system for handling contaminated process equipment

    International Nuclear Information System (INIS)

    Fisher, J.J.; Ward, C.R.; Schuler, T.F.

    1987-01-01

    E. I. du Pont de Nemours and Company is evaluating a unique eight-degree-of-freedom Telerobot manipulator to perform size-reduction and material handling operations on contaminated process equipment at the Savannah River Plant (SRP). The Telerobot will be installed in the proposed Transuranic (TRU) Waste Processing Facility, which is scheduled to be operational by 1990. A full-scale prototype Telerobot, manufactured by GCA Corporation, St. Paul, MN is being tested with other process equipment in the Components Test Facility at the Savannah River Laboratory (SRL). All telerobotic operations required in the TRU Waste Facility such as crate unpacking, equipment dismantling, material size-reduction, and selected maintenance operations are being tested. This paper discusses the major mechanical and control features of the Telerobot system. Several system enhancements were added by SRL, including a new quick-hand-change coupling and expanded software control functions. The new software enables a system operator to perform both teleoperated and automatic tasks through several operating modes. These enhancements, as well as future mechanical, control system, and software features, are reviewed

  2. SYSTEM

    Directory of Open Access Journals (Sweden)

    K. Swarnalatha

    2013-01-01

    Full Text Available Risk analysis of urban aquatic systems due to heavy metals turns significant due to their peculiar properties viz. persis tence, non-degradab ility, toxicity, and accumulation. Akkulam Veli (AV, an urba n tropical lake in south India is subjected to various environmental stresses due to multiple waste discharge, sand mining, developmental activities, tour ism related activitie s etc. Hence, a comprehensive approach is adopted for risk assessment using modified degree of contamination factor, toxicity units based on numerical sediment quality guidelines (SQGs, and potentialecological risk indices. The study revealed the presence of toxic metals such as Cr, C d, Pb and As and the lake is rated under ‘low ecological risk’ category.

  3. In-cell maintenance by manipulator arm with 3D workspace information recreated by laser rangefinder

    International Nuclear Information System (INIS)

    Kitamura, Akihiro; Nakai, Koji; Namekawa, Takashi; Watahiki, Masatoshi

    2011-01-01

    Highlights: → We developed a remote control system for maintenance of in-cell type fuel fabrication equipment. → The system display recreated three-dimensional information of the workspace from data obtained by laser rangefinder and conventional cameras. It has allowed us to operate a manipulator arm remotely with several control modes. → We implemented remote handling experiments using mock up equipment. Performance was compared for remote operation conducted using several different display and operation modes. → It was observed that integration of 3D information from the laser rangefinder reduced operation time and reinforced visual information during remote operation. - Abstract: We developed a remote control system for maintenance of in-cell type fuel fabrication equipment. The system display recreated three-dimensional information of the workspace from data obtained by laser rangefinder and conventional cameras. It has allowed us to operate a manipulator arm remotely with several control modes. In order to evaluate the effectiveness and usefulness of developed system, we implemented remote handling experiments using mock up equipment. Performance was compared for remote operation conducted using several different display and operation modes. We confirmed that the system is able to maintain in-cell fuel fabrication equipment in each display and operation mode. Times required to complete the remote operations were collected and compared in each mode. It was observed that integration of 3D information from the laser rangefinder reduced operation time and reinforced visual information during remote operation.

  4. Selecting a pharmacy layout design using a weighted scoring system.

    Science.gov (United States)

    McDowell, Alissa L; Huang, Yu-Li

    2012-05-01

    A weighted scoring system was used to select a pharmacy layout redesign. Facilities layout design techniques were applied at a local hospital pharmacy using a step-by-step design process. The process involved observing and analyzing the current situation, observing the current available space, completing activity flow charts of the pharmacy processes, completing communication and material relationship charts to detail which areas in the pharmacy were related to one another and how they were related, researching applications in other pharmacies or in scholarly works that could be beneficial, numerically defining space requirements for areas within the pharmacy, measuring the available space within the pharmacy, developing a set of preliminary designs, and modifying preliminary designs so they were all acceptable to the pharmacy staff. To select a final layout that could be implemented in the pharmacy, those layouts were compared via a weighted scoring system. The weighted aspect further allowed additional emphasis on categories based on their effect on pharmacy performance. The results produced a beneficial layout design as determined through simulated models of the pharmacy operation that more effectively allocated and strategically located space to improve transportation distances and materials handling, employee utilization, and ergonomics. Facilities layout designs for a hospital pharmacy were evaluated using a weighted scoring system to identify a design that was superior to both the current layout and alternative layouts in terms of feasibility, cost, patient safety, employee safety, flexibility, robustness, transportation distance, employee utilization, objective adherence, maintainability, usability, and environmental impact.

  5. Accelerating Acceptance of Fuel Cell Backup Power Systems - Final Report

    Energy Technology Data Exchange (ETDEWEB)

    Petrecky, James; Ashley, Christopher

    2014-07-21

    Since 2001, Plug Power has installed more than 800 stationary fuel cell systems worldwide. Plug Power’s prime power systems have produced approximately 6.5 million kilowatt hours of electricity and have accumulated more than 2.5 million operating hours. Intermittent, or backup, power products have been deployed with telecommunications carriers and government and utility customers in North and South America, Europe, the United Kingdom, Japan and South Africa. Some of the largest material handling operations in North America are currently using the company’s motive power units in fuel cell-powered forklifts for their warehouses, distribution centers and manufacturing facilities. The low-temperature GenSys fuel cell system provides remote, off-grid and primary power where grid power is unreliable or nonexistent. Built reliable and designed rugged, low- temperature GenSys delivers continuous or backup power through even the most extreme conditions. Coupled with high-efficiency ratings, low-temperature GenSys reduces operating costs making it an economical solution for prime power requirements. Currently, field trials at telecommunication and industrial sites across the globe are proving the advantages of fuel cells—lower maintenance, fuel costs and emissions, as well as longer life—compared with traditional internal combustion engines.

  6. Nanodiamonds and Their Applications in Cells.

    Science.gov (United States)

    Chipaux, Mayeul; van der Laan, Kiran J; Hemelaar, Simon R; Hasani, Masoumeh; Zheng, Tingting; Schirhagl, Romana

    2018-03-24

    Diamonds owe their fame to a unique set of outstanding properties. They combine a high refractive index, hardness, great stability and inertness, and low electrical but high thermal conductivity. Diamond defects have recently attracted a lot of attention. Given this unique list of properties, it is not surprising that diamond nanoparticles are utilized for numerous applications. Due to their hardness, they are routinely used as abrasives. Their small and uniform size qualifies them as attractive carriers for drug delivery. The stable fluorescence of diamond defects allows their use as stable single photon sources or biolabels. The magnetic properties of the defects make them stable spin qubits in quantum information. This property also allows their use as a sensor for temperature, magnetic fields, electric fields, or strain. This Review focuses on applications in cells. Different diamond materials and the special requirements for the respective applications are discussed. Methods to chemically modify the surface of diamonds and the different hurdles one has to overcome when working with cells, such as entering the cells and biocompatibility, are described. Finally, the recent developments and applications in labeling, sensing, drug delivery, theranostics, antibiotics, and tissue engineering are critically discussed. © 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  7. N-Acetylglucosamine Functions in Cell Signaling

    Directory of Open Access Journals (Sweden)

    James B. Konopka

    2012-01-01

    Full Text Available The amino sugar N-acetylglucosamine (GlcNAc is well known for the important structural roles that it plays at the cell surface. It is a key component of bacterial cell wall peptidoglycan, fungal cell wall chitin, and the extracellular matrix of animal cells. Interestingly, recent studies have also identified new roles for GlcNAc in cell signaling. For example, GlcNAc stimulates the human fungal pathogen Candida albicans to undergo changes in morphogenesis and expression of virulence genes. Pathogenic E. coli responds to GlcNAc by altering the expression of fimbriae and CURLI fibers that promote biofilm formation and GlcNAc stimulates soil bacteria to undergo changes in morphogenesis and production of antibiotics. Studies with animal cells have revealed that GlcNAc influences cell signaling through the posttranslational modification of proteins by glycosylation. O-linked attachment of GlcNAc to Ser and Thr residues regulates a variety of intracellular proteins, including transcription factors such as NFκB, c-myc, and p53. In addition, the specificity of Notch family receptors for different ligands is altered by GlcNAc attachment to fucose residues in the extracellular domain. GlcNAc also impacts signal transduction by altering the degree of branching of N-linked glycans, which influences cell surface signaling proteins. These emerging roles of GlcNAc as an activator and mediator of cellular signaling in fungi, animals, and bacteria will be the focus of this paper.

  8. 78 FR 46363 - Notice of Availability of the Draft Supplemental Environmental Impact Statement for the Palen...

    Science.gov (United States)

    2013-07-31

    ... resources, water resources, geological resources and hazards, hazardous materials handling, noise..., reliability, transmission system engineering and transmission line safety and nuisance. Before including your...

  9. REMOTE IN-CELL SAMPLING IMPROVEMENTS PROGRAM AT THESAVANNAH RIVER SITE (SRS) DEFENSE WASTE PROCESSING FACILITY (DWPF)

    International Nuclear Information System (INIS)

    Marzolf, A

    2007-01-01

    Remote Systems Engineering (RSE) of the Savannah River National Lab (SRNL) in combination with the Defense Waste Processing Facility(DWPF) Engineering and Operations has evaluated the existing equipment and processes used in the facility sample cells for 'pulling' samples from the radioactive waste stream and performing equipment in-cell repairs/replacements. RSE has designed and tested equipment for improving remote in-cell sampling evolutions and reducing the time required for in-cell maintenance of existing equipment. The equipment within the present process tank sampling system has been in constant use since the facility start-up over 17 years ago. At present, the method for taking samples within the sample cells produces excessive maintenance and downtime due to frequent failures relative to the sampling station equipment and manipulator. Location and orientation of many sampling stations within the sample cells is not conducive to manipulator operation. The overextension of manipulators required to perform many in-cell operations is a major cause of manipulator failures. To improve sampling operations and reduce downtime due to equipment maintenance, a Portable Sampling Station (PSS), wireless in-cell cameras, and new commercially available sampling technology has been designed, developed and/or adapted and tested. The uniqueness of the design(s), the results of the scoping tests, and the benefits relative to in-cell operation and reduction of waste are presented

  10. About novelty of radiation drug 'Storm in cells'

    International Nuclear Information System (INIS)

    Korchubekov, B.

    2005-01-01

    Drugs 'Storm in cells' is intended for treatment of infection wounds and burns in medical practice. The preparation represents the electro- activated mixture consisting uranium and thorium masses in the mumie base, table salt, activated carbon and water. Advantage of the drug 'Storm in cells' in comparison with prototype is increase of wound and burns repair effectiveness in 10-11 %

  11. Porosome: The Universal Secretory Portal in Cells

    Science.gov (United States)

    Jena, Bhanu

    2012-10-01

    In the past 50 years it was believed that during cell secretion, membrane-bound secretory vesicles completely merge at the cell plasma membrane resulting in the diffusion of intra-vesicular contents to the cell exterior and the compensatory retrieval of the excess membrane by endocytosis. This explanation made no sense or logic, since following cell secretion partially empty vesicles accumulate as demonstrated in electron micrographs. Furthermore, with the ``all or none'' mechanism of cell secretion by complete merger of secretory vesicle membrane at the cell plasma membrane, the cell is left with little regulation and control of the amount of content release. Moreover, it makes no sense for mammalian cells to possess such `all or none' mechanism of cell secretion, when even single-cell organisms have developed specialized and sophisticated secretory machinery, such as the secretion apparatus of Toxoplasma gondii, the contractile vacuoles in paramecium, or the various types of secretory structures in bacteria. Therefore, in 1993 in a News and Views article in Nature, E. Neher wrote ``It seems terribly wasteful that, during the release of hormones and neurotransmitters from a cell, the membrane of a vesicle should merge with the plasma membrane to be retrieved for recycling only seconds or minutes later.'' This conundrum in the molecular mechanism of cell secretion was finally resolved in 1997 following discovery of the ``Porosome,'' the universal secretory machinery in cells. Porosomes are supramolecular lipoprotein structures at the cell plasma membrane, where membrane-bound secretory vesicles transiently dock and fuse to release inravesicular contents to the outside during cell secretion. In the past decade, the composition of the porosome, its structure and dynamics at nm resolution and in real time, and its functional reconstitution into artificial lipid membrane, have all been elucidated. Since porosomes in exocrine and neuroendocrine cells measure 100-180 nm

  12. Assessment of manual material handling using Iranian MMH regulations and comparison with NIOSH equation and MAC method in one of the metal casting industries in Tehran, 2011

    Directory of Open Access Journals (Sweden)

    2013-08-01

    Result: Results showed an acceptable agreement between Iranian regulation and MAC method as well as Iranian regulation and NIOSH equation. However, no acceptable agreement was observed between MAC method and NIOSH equation.

  13. Aviation safety: hazardous materials handling. Hearing before a Subcommittee of the Committee on Government Operations, House of Representatives, Ninety-Sixth Congress, Second Session

    International Nuclear Information System (INIS)

    Anon.

    1980-01-01

    Statements concerning the safety of air transport of hazardous and radioactive materials presented before a Subcommittee of the Committee on Government Operations of the House of Representatives are presented. Statements of various personnel involved in air transport including the Air Line Pilots Association and the US Postal Service and the Professional Air Traffic Controllers Organization are presented for the record. Also included are appendices concerning the Minneapolis-Saint Paul Metropolitan Airport Commission Ordinance number 44, Air Line Pilots Association procedures for the safe transportation of passengers, and a personal statement concerning the handling procedures of radioactive materials by the US Postal Service

  14. Particle-in-cell simulations of Hall plasma thrusters

    Science.gov (United States)

    Miranda, Rodrigo; Ferreira, Jose Leonardo; Martins, Alexandre

    2016-07-01

    Hall plasma thrusters can be modelled using particle-in-cell (PIC) simulations. In these simulations, the plasma is described by a set of equations which represent a coupled system of charged particles and electromagnetic fields. The fields are computed using a spatial grid (i.e., a discretization in space), whereas the particles can move continuously in space. Briefly, the particle and fields dynamics are computed as follows. First, forces due to electric and magnetic fields are employed to calculate the velocities and positions of particles. Next, the velocities and positions of particles are used to compute the charge and current densities at discrete positions in space. Finally, these densities are used to solve the electromagnetic field equations in the grid, which are interpolated at the position of the particles to obtain the acting forces, and restart this cycle. We will present numerical simulations using software for PIC simulations to study turbulence, wave and instabilities that arise in Hall plasma thrusters. We have sucessfully reproduced a numerical simulation of a SPT-100 Hall thruster using a two-dimensional (2D) model. In addition, we are developing a 2D model of a cylindrical Hall thruster. The results of these simulations will contribute to improve the performance of plasma thrusters to be used in Cubesats satellites currenty in development at the Plasma Laboratory at University of Brasília.

  15. Transient processes in cell proliferation kinetics

    CERN Document Server

    Yakovlev, Andrej Yu

    1989-01-01

    A mathematician who has taken the romantic decision to devote himself to biology will doubtlessly look upon cell kinetics as the most simple and natural field of application for his knowledge and skills. Indeed, the thesaurus he is to master is not so complicated as, say, in molecular biology, the structural elements of the system, i. e. ceils, have been segregated by Nature itself, simple considerations of balance may be used for deducing basic equations, and numerous analogies in other areas of science also superficial add to one"s confidence. Generally speaking, this number of impression is correct, as evidenced by the very great theoretical studies on population kinetics, unmatched in other branches of mathematical biology. This, however, does not mean that mathematical theory of cell systems has traversed in its development a pathway free of difficulties or errors. The seeming ease of formalizing the phenomena of cell kinetics not infrequently led to the appearance of mathematical models lacking in adequ...

  16. Retrieval-travel-time model for free-fall-flow-rack automated storage and retrieval system

    Science.gov (United States)

    Metahri, Dhiyaeddine; Hachemi, Khalid

    2018-03-01

    Automated storage and retrieval systems (AS/RSs) are material handling systems that are frequently used in manufacturing and distribution centers. The modelling of the retrieval-travel time of an AS/RS (expected product delivery time) is practically important, because it allows us to evaluate and improve the system throughput. The free-fall-flow-rack AS/RS has emerged as a new technology for drug distribution. This system is a new variation of flow-rack AS/RS that uses an operator or a single machine for storage operations, and uses a combination between the free-fall movement and a transport conveyor for retrieval operations. The main contribution of this paper is to develop an analytical model of the expected retrieval-travel time for the free-fall flow-rack under a dedicated storage assignment policy. The proposed model, which is based on a continuous approach, is compared for accuracy, via simulation, with discrete model. The obtained results show that the maximum deviation between the continuous model and the simulation is less than 5%, which shows the accuracy of our model to estimate the retrieval time. The analytical model is useful to optimise the dimensions of the rack, assess the system throughput, and evaluate different storage policies.

  17. A Study of In-cell Television in a Closed Adult Male Prison: Governing Souls with In-cell Television

    OpenAIRE

    Knight, Victoria

    2012-01-01

    In-cell television is now a permanent feature of prisons in England and Wales, and a key part of the experience of modern incarceration. In-cell television was formally introduced in 1998 and its introduction took twelve years to complete across the prison estate. Its introduction was not informed by research and no formal evaluation of in-cell television in prisons has taken place. This thesis, therefore extends the small body of prisoner audience research with an exclusive focus on capturin...

  18. Standard guide for mechanical drive systems for remote operation in hot cell facilities

    CERN Document Server

    American Society for Testing and Materials. Philadelphia

    2010-01-01

    1.1 Intent: 1.1.1 The intent of this standard is to provide general guidelines for the design, selection, quality assurance, installation, operation, and maintenance of mechanical drive systems used in remote hot cell environments. The term mechanical drive systems used herein, encompasses all individual components used for imparting motion to equipment systems, subsystems, assemblies, and other components. It also includes complete positioning systems and individual units that provide motive power and any position indicators necessary to monitor the motion. 1.2 Applicability: 1.2.1 This standard is intended to be applicable to equipment used under one or more of the following conditions: 1.2.1.1 The materials handled or processed constitute a significant radiation hazard to man or to the environment. 1.2.1.2 The equipment will generally be used over a long-term life cycle (for example, in excess of two years), but equipment intended for use over a shorter life cycle is not excluded. 1.2.1.3 The ...

  19. Impact of Flexibility of Manufacturing System Components on Competitiveness of SMEs in Northern India

    Directory of Open Access Journals (Sweden)

    Shakun Preet Kaur

    2016-01-01

    Full Text Available The present manufacturing environment is characterized by a number of changes which poses challenges to a typical manufacturing unit. Time demands a shift from the traditional manufacturing strategies as they do not fit to present market competition. A flexible systems strategy has to be designed for remaining competitive in the market and perform well. For designing strategies and policies it is important to know the factors that influence performance of the system. The aim of this paper is to assess the impact of the flexibility of manufacturing system components on competitiveness of SMEs in northern India. A questionnaire based survey was conducted in the SMEs across northern India analysing three sectors namely automotive, machine tool and light engineering (mechanical components and equipment. The study contributes to the existing literature by empirically investigating the impact of machine, material handling and worker flexibility on competitiveness of manufacturing firms. This paper presents a Structural Equation Model displaying the impact of flexibility of manufacturing system components on competitiveness of SMEs.

  20. Safeguards systems analysis research and development and the practice of safeguards at DOE facilities

    International Nuclear Information System (INIS)

    Zack, N.R.; Thomas, K.E.; Markin, J.T.; Tape, J.W.

    1991-01-01

    Los Alamos Safeguards Systems Group personnel interact with Department of Energy (DOE) nuclear materials processing facilities in a number of ways. Among them are training courses, formal technical assistance such as developing information management or data analysis software, and informal ad hoc assistance especially in reviewing and commenting on existing facility safeguards technology and procedures. These activities are supported by the DOE Office of Safeguards and Security, DOE Operations Offices, and contractor organizations. Because of the relationships with the Operations Office and facility personnel, the Safeguards Systems Group research and development (R and D) staff have developed an understanding of the needs of the entire complex. Improved safeguards are needed in areas such as materials control activities, accountability procedures and techniques, systems analysis and evaluation methods, and material handling procedures. This paper surveys the generic needs for efficient and cost effective enhancements in safeguards technologies and procedures at DOE facilities, identifies areas where existing safeguards R and D products are being applied or could be applied, and sets a direction for future systems analysis R and D to address practical facility safeguards needs

  1. Advances in cell culture: anchorage dependence

    Science.gov (United States)

    Merten, Otto-Wilhelm

    2015-01-01

    Anchorage-dependent cells are of great interest for various biotechnological applications. (i) They represent a formidable production means of viruses for vaccination purposes at very large scales (in 1000–6000 l reactors) using microcarriers, and in the last decade many more novel viral vaccines have been developed using this production technology. (ii) With the advent of stem cells and their use/potential use in clinics for cell therapy and regenerative medicine purposes, the development of novel culture devices and technologies for adherent cells has accelerated greatly with a view to the large-scale expansion of these cells. Presently, the really scalable systems—microcarrier/microcarrier-clump cultures using stirred-tank reactors—for the expansion of stem cells are still in their infancy. Only laboratory scale reactors of maximally 2.5 l working volume have been evaluated because thorough knowledge and basic understanding of critical issues with respect to cell expansion while retaining pluripotency and differentiation potential, and the impact of the culture environment on stem cell fate, etc., are still lacking and require further studies. This article gives an overview on critical issues common to all cell culture systems for adherent cells as well as specifics for different types of stem cells in view of small- and large-scale cell expansion and production processes. PMID:25533097

  2. Global Particle-in-Cell Simulations of Mercury's Magnetosphere

    Science.gov (United States)

    Schriver, D.; Travnicek, P. M.; Lapenta, G.; Amaya, J.; Gonzalez, D.; Richard, R. L.; Berchem, J.; Hellinger, P.

    2017-12-01

    Spacecraft observations of Mercury's magnetosphere have shown that kinetic ion and electron particle effects play a major role in the transport, acceleration, and loss of plasma within the magnetospheric system. Kinetic processes include reconnection, the breakdown of particle adiabaticity and wave-particle interactions. Because of the vast range in spatial scales involved in magnetospheric dynamics, from local electron Debye length scales ( meters) to solar wind/planetary magnetic scale lengths (tens to hundreds of planetary radii), fully self-consistent kinetic simulations of a global planetary magnetosphere remain challenging. Most global simulations of Earth's and other planet's magnetosphere are carried out using MHD, enhanced MHD (e.g., Hall MHD), hybrid, or a combination of MHD and particle in cell (PIC) simulations. Here, 3D kinetic self-consistent hybrid (ion particle, electron fluid) and full PIC (ion and electron particle) simulations of the solar wind interaction with Mercury's magnetosphere are carried out. Using the implicit PIC and hybrid simulations, Mercury's relatively small, but highly kinetic magnetosphere will be examined to determine how the self-consistent inclusion of electrons affects magnetic reconnection, particle transport and acceleration of plasma at Mercury. Also the spatial and energy profiles of precipitating magnetospheric ions and electrons onto Mercury's surface, which can strongly affect the regolith in terms of space weathering and particle outflow, will be examined with the PIC and hybrid codes. MESSENGER spacecraft observations are used both to initiate and validate the global kinetic simulations to achieve a deeper understanding of the role kinetic physics play in magnetospheric dynamics.

  3. Prion propagation in cells expressing PrP glycosylation mutants.

    Science.gov (United States)

    Salamat, Muhammad K; Dron, Michel; Chapuis, Jérôme; Langevin, Christelle; Laude, Hubert

    2011-04-01

    Infection by prions involves conversion of a host-encoded cell surface protein (PrP(C)) to a disease-related isoform (PrP(Sc)). PrP(C) carries two glycosylation sites variably occupied by complex N-glycans, which have been suggested by previous studies to influence the susceptibility to these diseases and to determine characteristics of prion strains. We used the Rov cell system, which is susceptible to sheep prions, to generate a series of PrP(C) glycosylation mutants with mutations at one or both attachment sites. We examined their subcellular trafficking and ability to convert into PrP(Sc) and to sustain stable prion propagation in the absence of wild-type PrP. The susceptibility to infection of mutants monoglycosylated at either site differed dramatically depending on the amino acid substitution. Aglycosylated double mutants showed overaccumulation in the Golgi compartment and failed to be infected. Introduction of an ectopic glycosylation site near the N terminus fully restored cell surface expression of PrP but not convertibility into PrP(Sc), while PrP(C) with three glycosylation sites conferred cell permissiveness to infection similarly to the wild type. In contrast, predominantly aglycosylated molecules with nonmutated N-glycosylation sequons, produced in cells expressing glycosylphosphatidylinositol-anchorless PrP(C), were able to form infectious PrP(Sc). Together our findings suggest that glycosylation is important for efficient trafficking of anchored PrP to the cell surface and sustained prion propagation. However, properly trafficked glycosylation mutants were not necessarily prone to conversion, thus making it difficult in such studies to discern whether the amino acid changes or glycan chain removal most influences the permissiveness to prion infection.

  4. An accountancy system for nuclear materials control in research centres

    International Nuclear Information System (INIS)

    Buttler, R.; Bueker, H.; Vallee, J.

    1979-01-01

    The Nuclear Accountancy and Control System (NACS) was developed at KFA Juelich in accordance with the requirements of the Non-Proliferation Treaty. The main features are (1) recording of nuclear material in inventory items. These are combined to form batches wherever suitable; (2) extrapolation of accounting data as a replacement for detailed measurement of inventory items data. Recording and control of nuclear material are carried out on two levels with access to a common data bank. The lower level deals with nuclear materials handling plus internal management while on the upper level there is a central control point which is responsible for nuclear safeguarding within the entire research centre. By keeping the organizational and technical infrastructure it was possible to develop a system which is both economical and operator-oriented. In this system the emphasis of nuclear safeguarding is placed on the acquisition of the nuclear material inventory. As much consideration has been given to the interests of the various operational levels and organizational units as to internal and national regulations. Since it is part of the safeguarding and control system, access to the NACS must be restricted to a limited number of users only. Furthermore, it must include facilities for manual control in the form of records. Authorization for access must correspond with the various tasks of different user groups. All necessary data are acquired decentrally in the organizational units and entered via a terminal. It is available to the user groups on both levels through a central data bank. To meet all requirements, the NACS has been designed as an integrated, computer-assisted information system for the automated processing of extensive and multi-level nuclear materials data. As part of the preventive measures entailed with nuclear safeguarding, the accountancy system enables the operator of a nuclear plant to furnish proof of non-diversion of nuclear material. (author)

  5. Sparse grid techniques for particle-in-cell schemes

    Science.gov (United States)

    Ricketson, L. F.; Cerfon, A. J.

    2017-02-01

    We propose the use of sparse grids to accelerate particle-in-cell (PIC) schemes. By using the so-called ‘combination technique’ from the sparse grids literature, we are able to dramatically increase the size of the spatial cells in multi-dimensional PIC schemes while paying only a slight penalty in grid-based error. The resulting increase in cell size allows us to reduce the statistical noise in the simulation without increasing total particle number. We present initial proof-of-principle results from test cases in two and three dimensions that demonstrate the new scheme’s efficiency, both in terms of computation time and memory usage.

  6. Cost of radon-barrier systems for uranium mill tailings

    International Nuclear Information System (INIS)

    Baker, E.G.; Hartley, J.N.

    1982-08-01

    This report deals specifically with the cost of three types of radon barrier systems, earthen covers, asphalt emulsion covers, and multilayer covers, which could meet standards proposed by the Environmental Protection Agency to stabilize uranium mill tailings located primarily in the western US. In addition, the report includes a sensitivity analysis of various factors which significantly effect the overall cost of the three systems. These analyses were based on a generic disposal site. Four different 3m thick earthen covers were tested and cost an average of $27/m 2 . The least expensive earthen cover cost was about $21/m 2 . The asphalt cover system (6 to 7 cm of asphalt topped with 0.6m of overburden) cost about $28/m 2 . The four multilayer covers averaged $57/m 2 , but materials handling problems encountered during the test inflated this cost above what was anticipated and significant cost reductions should be possible. The least expensive multilayer cover cost $43/m 2 . Based on the results of the Grand Junction field test we estimated the cost of covering the tailings from three high priority sites, Durango, Shiprock, and Salt Lake City (Vitro). The cost of a 3m earthen cover ranged from $18 to 33/m 2 for the seven disposal sites (two or three at each location) studied. The cost of asphalt cover systems were $23 to 28/m 2 and the multilayer cover costs were between $31 to 36/m 2 . The earthen cover costs are less than the Grand Junction field test cost primarily because cover material is available at or near most of the disposal sites selected. Earthen material was imported from 6 to 10 miles for the field test. Assuming more efficienct utilization of materials significantly reduced the cost of the multilayer covers

  7. Rapid-mixing studies on the time-scale of radiation damage in cells

    International Nuclear Information System (INIS)

    Adams, G.E.; Michael, B.D.; Asquith, J.C.; Shenoy, M.A.; Watts, M.E.; Whillans, D.W.

    1975-01-01

    Rapid mixing studies were performed to determine the time scale of radiation damage in cells. There is evidence that the sensitizing effects of oxygen and other chemical dose-modifying agents on the response of cells to ionizing radiation involve fast free-radical processes. Fast response technique studies in bacterial systems have shown that extremely fast processes occur when the bacteria are exposed to oxygen or other dose-modifying agents during irradiation. The time scales observed were consistent with the involvement of fast free-radical reactions in the expression of these effects

  8. Recent advances in cell-based therapy for Parkinson disease

    DEFF Research Database (Denmark)

    Astradsson, Arnar; Cooper, Oliver; Vinuela, Angel

    2008-01-01

    In this review, the authors discuss recent advances in the field of cell therapy for Parkinson disease (PD). They compare and contrast recent clinical trials using fetal dopaminergic neurons. They attribute differences in cell preparation techniques, cell type specification, and immunosuppression...

  9. A multiresolution remeshed Vortex-In-Cell algorithm using patches

    DEFF Research Database (Denmark)

    Rasmussen, Johannes Tophøj; Cottet, Georges-Henri; Walther, Jens Honore

    2011-01-01

    We present a novel multiresolution Vortex-In-Cell algorithm using patches of varying resolution. The Poisson equation relating the fluid vorticity and velocity is solved using Fast Fourier Transforms subject to free space boundary conditions. Solid boundaries are implemented using the semi...

  10. iPS-Cinderella Story in Cell Biology

    Directory of Open Access Journals (Sweden)

    Editorial

    2010-01-01

    Full Text Available As we step through the frontiers of modern Science, we are all witnesses to the Cinderella story repeating itself in the form of the iPS. The process of re-programming adult somatic cells to derive Induced Pluripotent stem cells (iPS with the wand of transcription factors and then differentiating them back to adult somatic cells resembles the transformation of Cinderella from a Cinder girl to princess and back to a Cinder girl after the ball; but the iPS-Cinderella is the most fascinating thing ever in cell biology!From the day iPS first made its headlines when it was first produced by Shinya Yamanaka at Kyoto University in Japan, Stem Cell scientists all over the world are re- doing their experiments so far done using other sources like embryonic and adult Stem cells with the iPS cells exploring their potential to the fullest. A Stem Cell science news page without this magic word of iPS is difficult to imagine these days and Scientists have been successful in growing most of the adult Cell types from iPS cells.iPS cells was the key to solve the problems of Immune rejection and Immunosupression required when using other allogeneic Stem cell types which had baffled scientists previously. But the issues raised by scientists about the use of viruses and Oncogenes in producing iPS cells were made groundless when scientists in February 2008 published the discovery of a technique that could remove oncogenes after the induction of pluripotency and now it is possible to induce pluripotency using plasmid transfection, piggyback transposon system and piggyback transposon system combined with a non viral vector system. The word of the day is pIPS which are protein-induced Pluripotent stem cells which are iPS cells that were generated without any genetic alteration of the adult cell. This research by the group of Sheng Ding in La Jolla, California made public in April 2009 showed that the generation of poly-arginine anchors was sufficient to induce

  11. Realistic simulations of a cyclotron spiral inflector within a particle-in-cell framework

    Science.gov (United States)

    Winklehner, Daniel; Adelmann, Andreas; Gsell, Achim; Kaman, Tulin; Campo, Daniela

    2017-12-01

    We present an upgrade to the particle-in-cell ion beam simulation code opal that enables us to run highly realistic simulations of the spiral inflector system of a compact cyclotron. This upgrade includes a new geometry class and field solver that can handle the complicated boundary conditions posed by the electrode system in the central region of the cyclotron both in terms of particle termination, and calculation of self-fields. Results are benchmarked against the analytical solution of a coasting beam. As a practical example, the spiral inflector and the first revolution in a 1 MeV /amu test cyclotron, located at Best Cyclotron Systems, Inc., are modeled and compared to the simulation results. We find that opal can now handle arbitrary boundary geometries with relative ease. Simulated injection efficiencies and beam shape compare well with measured efficiencies and a preliminary measurement of the beam distribution after injection.

  12. DESIGN AND CONSTRUCTION OF A FORCE-REFLECTING TELEOPERATION SYSTEM

    Energy Technology Data Exchange (ETDEWEB)

    M.A. Ebadian, Ph.D.

    1999-01-01

    For certain applications, such as space servicing, undersea operations, and hazardous material handling tasks in nuclear reactors, the environments can be uncertain, complex, and hazardous. Lives may be in danger if humans were to work under these conditions. As a result, a man-machine system--a teleoperator system--has been developed to work in these types of environments. In a typical teleoperator system, the actual system operates at a remote site; the operator located away from this system usually receives visual information from a video image and/or graphical animation on the computer screen. Additional feedback, such as aural and force information, can significantly enhance performance of the system. Force reflection is a type of feedback in which forces experienced by the remote manipulator are fed back to the manual controller. Various control methods have been proposed for implementation on a teleoperator system. In order to examine different control schemes, a one Degree-Of-Freedom (DOF) Force-Reflecting Manual Controller (FRMC) is constructed and integrated into a PC. The system parameters are identified and constructed as a mathematical model. The Proportional-Integral-Derivative (PID) and fuzzy logic controllers are developed and tested experimentally. Numerical simulation results obtained from the mathematical model are compared with those of experimental data for both types of controllers. In addition, the concept of a telesensation system is introduced. A telesensation system is an advanced teleoperator system that attempts to provide the operator with sensory feedback. In this context, a telesensation system integrates the use of a Virtual Reality (VR) unit, FRMC, and Graphical User Interface (GUI). The VR unit is used to provide the operator with a 3-D visual effect. Various commercial VR units are reviewed and features compared for use in a telesensation system. As for the FRMC, the conceptual design of a 3-DOF FRMC is developed in an effort to

  13. DESIGN AND CONSTRUCTION OF A FORCE-REFLECTING TELEOPERATION SYSTEM

    International Nuclear Information System (INIS)

    Ebadian, M.A.

    1999-01-01

    For certain applications, such as space servicing, undersea operations, and hazardous material handling tasks in nuclear reactors, the environments can be uncertain, complex, and hazardous. Lives may be in danger if humans were to work under these conditions. As a result, a man-machine system--a teleoperator system--has been developed to work in these types of environments. In a typical teleoperator system, the actual system operates at a remote site; the operator located away from this system usually receives visual information from a video image and/or graphical animation on the computer screen. Additional feedback, such as aural and force information, can significantly enhance performance of the system. Force reflection is a type of feedback in which forces experienced by the remote manipulator are fed back to the manual controller. Various control methods have been proposed for implementation on a teleoperator system. In order to examine different control schemes, a one Degree-Of-Freedom (DOF) Force-Reflecting Manual Controller (FRMC) is constructed and integrated into a PC. The system parameters are identified and constructed as a mathematical model. The Proportional-Integral-Derivative (PID) and fuzzy logic controllers are developed and tested experimentally. Numerical simulation results obtained from the mathematical model are compared with those of experimental data for both types of controllers. In addition, the concept of a telesensation system is introduced. A telesensation system is an advanced teleoperator system that attempts to provide the operator with sensory feedback. In this context, a telesensation system integrates the use of a Virtual Reality (VR) unit, FRMC, and Graphical User Interface (GUI). The VR unit is used to provide the operator with a 3-D visual effect. Various commercial VR units are reviewed and features compared for use in a telesensation system. As for the FRMC, the conceptual design of a 3-DOF FRMC is developed in an effort to

  14. Proceedings of the Human Factors Society 31st annual meeting

    Energy Technology Data Exchange (ETDEWEB)

    1987-01-01

    Topics covered include: operator/user modeling; automation safety; manual materials handling (includes a paper on materials handling in underground coal mining); impact of human performance on system performance; transportation safety; ergonomic design; mining safety, sleep deprivation and stressors; environmental design; training evaluation and research; and design and evaluation.

  15. Fleet servicing facilities for servicing, maintaining, and testing rail and truck radioactive waste transport systems: functional requirements, technical design concepts and options cost estimates and comparisons

    International Nuclear Information System (INIS)

    Watson, C.D.; Hudson, B.J.; Keith, D.A.; Preston, M.K. Jr.; McCreery, P.N.; Knox, W.; Easterling, E.M.; Lamprey, A.S.; Wiedemann, G.

    1980-05-01

    This is a resource document which examines feasibility design concepts and feasibility studies of a Fleet Servicing Facility (FSF). Such a facility is intended to be used for routine servicing, preventive maintenance, and for performing requalification license compliance tests and inspections, minor repairs, and decontamination of both the transportation casks and their associated rail cars or tractor-trailers. None of the United States' waste handling plants presently receiving radioactive wastes have an on-site FSF, nor is there an existing third party facility providing these services. This situation has caused the General Accounting Office to express concern regarding the quality of waste transport system maintenance once the system is placed into service. Thus, a need is indicated for FSF's, or their equivalent, at various radioactive materials receiving sites. In this report, three forms of FSF's solely for spent fuel transport systems were examined: independent, integrated, and colocated. The independent concept was already the subject of a detailed report and is extensively referenced in this document so that capital cost comparisons of the three concepts could be made. These facilities probably could service high-level, intermediate-level, low-level, or other waste transportation systems with minor modification, but this study did not include any system other than spent fuel. Both the Integrated and Colocated concepts were assumed to be associated with some radioactive materials handling facility such as an AFR repository

  16. Role of Geminin in cell fate determination of hematopoietic stem cells (HSCs).

    Science.gov (United States)

    Yasunaga, Shin'ichiro; Ohno, Yoshinori; Shirasu, Naoto; Zhang, Bo; Suzuki-Takedachi, Kyoko; Ohtsubo, Motoaki; Takihara, Yoshihiro

    2016-09-01

    Geminin exerts two distinct molecular roles. Geminin negatively regulates DNA replication licensing through the direct interaction with Cdt1 to prevent re-replication in proliferating cells. Geminin also regulates chromatin remodeling through the direct interaction with Brahma/Brg1 to maintain undifferentiated states of stem cells. We previously uncovered that Polycomb-group complex 1 and Hoxb4/Hoxa9, well-known intrinsic factors that are essential for maintaining the hematopoietic stem cell (HSC) activity, alternatively act as ubiquitin-proteasome systems for Geminin protein to reduce the protein expression level, and sustain the HSC activity. Thus, Geminin is presumed to play an important role in determining cell fate, i.e., turning on and off cellular quiescence and proliferation/differentiation, in HSCs. We recently generated recombinant cell-penetrating Geminin (CP-Geminin), enabling rapid incorporation and withdraw of Geminin protein in cells. CP-Geminin may be useful in regulating the cell cycle and chromatin configuration. In this article, we summarize current information on the molecular functions of Geminin and the regulatory system for Geminin protein expression, and argue for the molecular role of Geminin in cell fate determination of HSCs, and future perspective of a new technology for manipulating the activities of HSCs and cancer stem cells (CSCs).

  17. Raman spectroscopy for DNA quantification in cell nucleus.

    Science.gov (United States)

    Okotrub, K A; Surovtsev, N V; Semeshin, V F; Omelyanchuk, L V

    2015-01-01

    Here we demonstrate the feasibility of a novel approach to quantify DNA in cell nuclei. This approach is based on spectroscopy analysis of Raman light scattering, and avoids the problem of nonstoichiometric binding of dyes to DNA, as it directly measures the signal from DNA. Quantitative analysis of nuclear DNA contribution to Raman spectrum could be reliably performed using intensity of a phosphate mode at 1096 cm(-1) . When compared to the known DNA standards from cells of different animals, our results matched those values at error of 10%. We therefore suggest that this approach will be useful to expand the list of DNA standards, to properly adjust the duration of hydrolysis in Feulgen staining, to assay the applicability of fuchsines for DNA quantification, as well as to measure DNA content in cells with complex hydrolysis patterns, when Feulgen densitometry is inappropriate. © 2014 International Society for Advancement of Cytometry.

  18. The replacement of serum by hormones in cell culture media.

    Science.gov (United States)

    Sato, G; Hayashi, I

    1976-12-01

    The replacement of serum by hormones in cell culture media. (Reemplazo del suero por hormonas en el medio de cultivo de células). Arch. Biol. Med. Exper. 10: 120-121, 1976. The serum used in cell culture media can be replaced by a mixture of hormones and some accesory blood factors. The pituitary cell line GH3 can be grown in a medium in which serum is replaced by triiodothyronine, transferrin, parathormone, tyrotrophin releasing hormone and somatomedins. Hela and BHK cell strains can also be grown in serum free medium supplemented with hormones. Each cell type appears to have different hormonal requirements yet it may found that some hormones are required for most cell types.

  19. Limits of validity of photon-in-cell simulation techniques

    International Nuclear Information System (INIS)

    Reitsma, A. J. W.; Jaroszynski, D. A.

    2008-01-01

    A comparison is made between two reduced models for studying laser propagation in underdense plasma; namely, photon kinetic theory and the slowly varying envelope approximation. Photon kinetic theory is a wave-kinetic description of the electromagnetic field where the motion of quasiparticles in photon coordinate-wave number phase space is described by the ray-tracing equations. Numerically, the photon kinetic theory is implemented with standard particle-in-cell techniques, which results in a so-called photon-in-cell code. For all the examples presented in this paper, the slowly varying envelope approximation is accurate and therefore discrepancies indicate the failure of photon kinetic approximation for these cases. Possible remedies for this failure are discussed at the end of the paper

  20. Milk-run kanban system for raw printed circuit board withdrawal to surface-mounted equipment

    Directory of Open Access Journals (Sweden)

    Swee Li Chee

    2012-12-01

    Full Text Available Purpose:  The paper aims to present a case study and later simulation analysis on a kanban system that incorporating milk-run operation to draw in raw material to the process. Design/methodology/approach:  Data collection at the case study company for ten weeks followed by a process study called value stream mapping. The proposed kanban model is simulated to test its various performances including total output, average flow time, average work-in-process, SME utilization, and average waiting time. Response surface methodology is adopted to generate suitable representative regression models.  Findings: For all performance measures, simulation results showed that the proposed system consistently outperforms the push system currently practiced. Second, the system indicates the advantages of leveling, particularly in the event of machine failure and blockage. Third, operator in the proposed kanban system has a lower utilization, even with the additional material handling task.   Research limitations/implications: This study only begins to reveal the implication of leveling for production control on multi-machine scenario. The simulation of the system is solely based only the case study. The control parameters critical to the case study, were naturally used. The furtherance of the research should include generalizing the system and devising the respective methodology to facilitate wider applications. Practical implications: Originality/value:  The kanban system is proposed in the light of conflicting interests in handling the surface mounting and the related upstream processes. Such aspect is common to electronics assembly industry.

  1. Particle In Cell Codes on Highly Parallel Architectures

    Science.gov (United States)

    Tableman, Adam

    2014-10-01

    We describe strategies and examples of Particle-In-Cell Codes running on Nvidia GPU and Intel Phi architectures. This includes basic implementations in skeletons codes and full-scale development versions (encompassing 1D, 2D, and 3D codes) in Osiris. Both the similarities and differences between Intel's and Nvidia's hardware will be examined. Work supported by grants NSF ACI 1339893, DOE DE SC 000849, DOE DE SC 0008316, DOE DE NA 0001833, and DOE DE FC02 04ER 54780.

  2. Alternative mitochondrial functions in cell physiopathology: beyond ATP production

    Directory of Open Access Journals (Sweden)

    Kowaltowski A.J.

    2000-01-01

    Full Text Available It is well known that mitochondria are the main site for ATP generation within most tissues. However, mitochondria also participate in a surprising number of alternative activities, including intracellular Ca2+ regulation, thermogenesis and the control of apoptosis. In addition, mitochondria are the main cellular generators of reactive oxygen species, and may trigger necrotic cell death under conditions of oxidative stress. This review concentrates on these alternative mitochondrial functions, and their role in cell physiopathology.

  3. Structure of the nucleoid in cells of Streptococcus faecalis.

    OpenAIRE

    Daneo-Moore, L; Dicker, D; Higgins, M L

    1980-01-01

    The structure of the nucleoid of Streptococcus faecalis (ATCC 9790) was examined and compared in the unfixed and fixed states by immersive refractometry and electron microscopy. It appears from these studies that the nucleoid structure is much more centralized in unfixed chloramphenicol-treated (stationary-phase) cells than it is in cells in the exponential phase of growth. The more dispersed configuration of the exponential-phase nucleoid could be preserved by fixation in glutaraldehyde, but...

  4. A New Method for Calculating Counts in Cells

    Science.gov (United States)

    Szapudi, István

    1998-04-01

    In the near future, a new generation of CCD-based galaxy surveys will enable high-precision determination of the N-point correlation functions. The resulting information will help to resolve the ambiguities associated with two-point correlation functions, thus constraining theories of structure formation, biasing, and Gaussianity of initial conditions independently of the value of Ω. As one of the most successful methods of extracting the amplitude of higher order correlations is based on measuring the distribution of counts in cells, this work presents an advanced way of measuring it with unprecedented accuracy. Szapudi & Colombi identified the main sources of theoretical errors in extracting counts in cells from galaxy catalogs. One of these sources, termed as measurement error, stems from the fact that conventional methods use a finite number of sampling cells to estimate counts in cells. This effect can be circumvented by using an infinite number of cells. This paper presents an algorithm, which in practice achieves this goal; that is, it is equivalent to throwing an infinite number of sampling cells in finite time. The errors associated with sampling cells are completely eliminated by this procedure, which will be essential for the accurate analysis of future surveys.

  5. Kinetics of Cu (II) separation by ion flotation techniques, in cells with flexible spargers

    International Nuclear Information System (INIS)

    Reyes, M.; Tavera, F. J.; Escudero, R.; Patino, F.; Salinas, E.; Rivera, I.

    2010-01-01

    This research studies and experimentally determines the kinetic parameters and effect of modifying the hydrodynamics and chemical conditions of the air-liquid dispersions during the Cu (II) extraction by ion flotation techniques in cells with porous spargers. Results show that the elimination of Cu (II) from solution can be carried out by ion flotation in one stage, obtaining efficiencies of 68% and 56% for the flat and cylindrical sparger respectively with a xanthate concentration of 0,02 g/l. In multistage systems five cells, recoveries over 92 % were achieved for both sparger geometries. The behavior of the flotation apparent kinetic constant is linear to the parameters that characterize dispersion (Jg, eg y Db), until a point is achieved where the process instability makes the system inoperable. The results show that removing base metal ions by ion flotation is strongly affected by the following factors: collector concentration [C], Jg, eg, Db, Jl and Sb. (Author) 20 refs

  6. Ef: Software for Nonrelativistic Beam Simulation by Particle-in-Cell Algorithm

    Directory of Open Access Journals (Sweden)

    Boytsov A. Yu.

    2018-01-01

    Full Text Available Understanding of particle dynamics is crucial in construction of electron guns, ion sources and other types of nonrelativistic beam devices. Apart from external guiding and focusing systems, a prominent role in evolution of such low-energy beams is played by particle-particle interaction. Numerical simulations taking into account these effects are typically accomplished by a well-known particle-in-cell method. In practice, for convenient work a simulation program should not only implement this method, but also support parallelization, provide integration with CAD systems and allow access to details of the simulation algorithm. To address the formulated requirements, development of a new open source code - Ef - has been started. It's current features and main functionality are presented. Comparison with several analytical models demonstrates good agreement between the numerical results and the theory. Further development plans are discussed.

  7. Ef: Software for Nonrelativistic Beam Simulation by Particle-in-Cell Algorithm

    Science.gov (United States)

    Boytsov, A. Yu.; Bulychev, A. A.

    2018-04-01

    Understanding of particle dynamics is crucial in construction of electron guns, ion sources and other types of nonrelativistic beam devices. Apart from external guiding and focusing systems, a prominent role in evolution of such low-energy beams is played by particle-particle interaction. Numerical simulations taking into account these effects are typically accomplished by a well-known particle-in-cell method. In practice, for convenient work a simulation program should not only implement this method, but also support parallelization, provide integration with CAD systems and allow access to details of the simulation algorithm. To address the formulated requirements, development of a new open source code - Ef - has been started. It's current features and main functionality are presented. Comparison with several analytical models demonstrates good agreement between the numerical results and the theory. Further development plans are discussed.

  8. A thiopurine drug inhibits West Nile virus production in cell culture, but not in mice.

    Science.gov (United States)

    Lim, Pei-Yin; Keating, Julie A; Hoover, Spencer; Striker, Rob; Bernard, Kristen A

    2011-01-01

    Many viruses within the Flavivirus genus cause significant disease in humans; however, effective antivirals against these viruses are not currently available. We have previously shown that a thiopurine drug, 6-methylmercaptopurine riboside (6MMPr), inhibits replication of distantly related viruses within the Flaviviridae family in cell culture, including bovine viral diarrhea virus and hepatitis C virus replicon. Here we further examined the potential antiviral effect of 6MMPr on several diverse flaviviruses. In cell culture, 6MMPr inhibited virus production of yellow fever virus, dengue virus-2 (DENV-2) and West Nile virus (WNV) in a dose-dependent manner, and DENV-2 was significantly more sensitive to 6MMPr treatment than WNV. We then explored the use of 6MMPr as an antiviral against WNV in an immunocompetent mouse model. Once a day treatment of mice with 0.5 mg 6MMPr was just below the toxic dose in our mouse model, and this dose was used in subsequent studies. Mice were treated with 6MMPr immediately after subcutaneous inoculation with WNV for eight consecutive days. Treatment with 6MMPr exacerbated weight loss in WNV-inoculated mice and did not significantly affect mortality. We hypothesized that 6MMPr has low bioavailability in the central nervous system (CNS) and examined the effect of pre-treatment with 6MMPr on viral loads in the periphery and CNS. Pre-treatment with 6MMPr had no significant effect on viremia or viral titers in the periphery, but resulted in significantly higher viral loads in the brain, suggesting that the effect of 6MMPr is tissue-dependent. In conclusion, despite being a potent inhibitor of flaviviruses in cell culture, 6MMPr was not effective against West Nile disease in mice; however, further studies are warranted to reduce the toxicity and/or improve the bioavailability of this potential antiviral drug.

  9. A thiopurine drug inhibits West Nile virus production in cell culture, but not in mice.

    Directory of Open Access Journals (Sweden)

    Pei-Yin Lim

    Full Text Available Many viruses within the Flavivirus genus cause significant disease in humans; however, effective antivirals against these viruses are not currently available. We have previously shown that a thiopurine drug, 6-methylmercaptopurine riboside (6MMPr, inhibits replication of distantly related viruses within the Flaviviridae family in cell culture, including bovine viral diarrhea virus and hepatitis C virus replicon. Here we further examined the potential antiviral effect of 6MMPr on several diverse flaviviruses. In cell culture, 6MMPr inhibited virus production of yellow fever virus, dengue virus-2 (DENV-2 and West Nile virus (WNV in a dose-dependent manner, and DENV-2 was significantly more sensitive to 6MMPr treatment than WNV. We then explored the use of 6MMPr as an antiviral against WNV in an immunocompetent mouse model. Once a day treatment of mice with 0.5 mg 6MMPr was just below the toxic dose in our mouse model, and this dose was used in subsequent studies. Mice were treated with 6MMPr immediately after subcutaneous inoculation with WNV for eight consecutive days. Treatment with 6MMPr exacerbated weight loss in WNV-inoculated mice and did not significantly affect mortality. We hypothesized that 6MMPr has low bioavailability in the central nervous system (CNS and examined the effect of pre-treatment with 6MMPr on viral loads in the periphery and CNS. Pre-treatment with 6MMPr had no significant effect on viremia or viral titers in the periphery, but resulted in significantly higher viral loads in the brain, suggesting that the effect of 6MMPr is tissue-dependent. In conclusion, despite being a potent inhibitor of flaviviruses in cell culture, 6MMPr was not effective against West Nile disease in mice; however, further studies are warranted to reduce the toxicity and/or improve the bioavailability of this potential antiviral drug.

  10. ACTIVITY-BASED COSTING DAN SIMULATED ANNEALING UNTUK PENCARIAN RUTE PADA FLEXIBLE MANUFACTURING SYSTEMS

    Directory of Open Access Journals (Sweden)

    Gregorius Satia Budhi

    2003-01-01

    Full Text Available Flexible Manufacturing System (FMS is a manufacturing system that is formed from several Numerical Controlled Machines combine with material handling system, so that different jobs can be worked by different machines sequences. FMS combine the high productivity and flexibility of Transfer Line and Job Shop manufacturing system. In this reasearch, Activity-Based Costing(ABC approach was used as the weight to search the operation route in the proper machine, so that the total production cost can be optimized. The search method that was used in this experiment is Simulated Annealling, a variant form Hill Climbing Search method. An ideal operation time to proses a part was used as the annealling schedule. From the empirical test, it could be proved that the use of ABC approach and Simulated Annealing to search the route (routing process can optimize the Total Production Cost. In the other hand, the use of ideal operation time to process a part as annealing schedule can control the processing time well. Abstract in Bahasa Indonesia : Flexible Manufacturing System (FMS adalah sistem manufaktur yang tersusun dari mesin-mesin Numerical Control (NC yang dikombinasi dengan Sistem Penanganan Material, sehingga job-job berbeda dikerjakan oleh mesin-mesin dengan alur yang berlainan. FMS menggabungkan produktifitas dan fleksibilitas yang tinggi dari Sistem Manufaktur Transfer Line dan Job Shop. Pada riset ini pendekatan Activity-Based Costing (ABC digunakan sebagai bobot / weight dalam pencarian rute operasi pada mesin yang tepat, untuk lebih mengoptimasi biaya produksi secara keseluruhan. Adapun metode Searching yang digunakan adalah Simulated Annealing yang merupakan varian dari metode searching Hill Climbing. Waktu operasi ideal untuk memproses sebuah part digunakan sebagai Annealing Schedulenya. Dari hasil pengujian empiris dapat dibuktikan bahwa penggunaan pendekatan ABC dan Simulated Annealing untuk proses pencarian rute (routing dapat lebih

  11. Particle-in-cell Simulations with Kinetic Electrons

    International Nuclear Information System (INIS)

    Lewandowski, J.L.V.

    2004-01-01

    A new scheme, based on an exact separation between adiabatic and nonadiabatic electron responses, for particle-in-cell (PIC) simulations of drift-type modes is presented. The (linear and nonlinear) elliptic equations for the scalar fields are solved using a multi-grid solver. The new scheme yields linear growth rates in excellent agreement with theory and it is shown to conserve energy well into the nonlinear regime. It is also demonstrated that simulations with few electrons are reliable and accurate, suggesting that large-scale, PIC simulations with electron dynamics in toroidal geometry (e.g., tokamaks and stellarators plasmas) are within reach of present-day massively parallel supercomputers

  12. Optimized Loading for Particle-in-cell Gyrokinetic Simulations

    International Nuclear Information System (INIS)

    Lewandowski, J.L.V.

    2004-01-01

    The problem of particle loading in particle-in-cell gyrokinetic simulations is addressed using a quadratic optimization algorithm. Optimized loading in configuration space dramatically reduces the short wavelength modes in the electrostatic potential that are partly responsible for the non-conservation of total energy; further, the long wavelength modes are resolved with good accuracy. As a result, the conservation of energy for the optimized loading is much better that the conservation of energy for the random loading. The method is valid for any geometry and can be coupled to optimization algorithms in velocity space

  13. DESIGN OF SMALL AUTOMATION WORK CELL SYSTEM DEMONSTRATIONS

    International Nuclear Information System (INIS)

    TURNER, C.; PEHL, J.

    2000-01-01

    Laboratory (LANL), which are considered to be representative of nuclear material handling glove box operations, the UTRRG has developed three task demonstration concepts to evaluate modular small automation systems. These demonstrations, utilizing 2 to 3 degree-of-freedom systems, include container movement, material transfer via pouring, and container loading. Based on an analysis of the ARIES pilot line specifications, these three simple demonstrations are considered to be similar to approximately 50% of the tasks in the ARIES pilot line. Since these task demonstrations functionally represent basic tasks, they are representative of the current potential of modular small automation technology to a wide spectrum of hazardous applications extending beyond the ARIES pilot line. The selection of these three demonstrations, their design and their effectiveness in demonstrating the potential of modular small automation technology are discussed

  14. Evidence for P-Glycoprotein Involvement in Cell Volume Regulation Using Coulter Sizing in Flow Cytometry

    Directory of Open Access Journals (Sweden)

    Jennifer Pasquier

    2015-06-01

    Full Text Available The regulation of cell volume is an essential function that is coupled to a variety of physiological processes such as receptor recycling, excitability and contraction, cell proliferation, migration, and programmed cell death. Under stress, cells undergo emergency swelling and respond to such a phenomenon with a regulatory volume decrease (RVD where they release cellular ions, and other osmolytes as well as a concomitant loss of water. The link between P-glycoprotein, a transmembrane transporter, and cell volume regulation is controversial, and changes in cells volume are measured using microscopy or electrophysiology. For instance, by using the patch-clamp method, our team demonstrated that chloride currents activated in the RVD were more intense and rapid in a breast cancer cell line overexpressing the P-glycoprotein (P-gp. The Cell Lab Quanta SC is a flow cytometry system that simultaneously measures electronic volume, side scatter and three fluorescent colors; altogether this provides unsurpassed population resolution and accurate cell counting. Therefore, here we propose a novel method to follow cellular volume. By using the Coulter-type channel of the cytometer Cell Lab Quanta SC MPL (multi-platform loading, we demonstrated a role for the P-gp during different osmotic treatments, but also a differential activity of the P-gp through the cell cycle. Altogether, our data strongly suggests a role of P-gp in cell volume regulation.

  15. Enhanced taurine release in cell-damaging conditions in the developing and ageing mouse hippocampus.

    Science.gov (United States)

    Saransaari, P; Oja, S S

    1997-08-01

    Taurine has been shown to be essential for neuronal development and survival in the central nervous system. The release of preloaded [3H]taurine was studied in hippocampal slices from seven-day-, three-month- and 18-22-month-old mice in cell-damaging conditions. The slices were superfused in hypoxic, hypoglycemic and ischemic conditions and exposed to free radicals and oxidative stress. The release of taurine was greatly enhanced in the above conditions in all age groups, except in oxidative stress. The release was large in ischemia, particularly in the hippocampus of aged mice. Potassium stimulation was still able to release taurine in cell-damaging conditions in immature mice, whereas in adult and aged animals the release was so substantial that this additional stimulus failed to work. Taurine release was partially Ca2+-dependent in all cases. The massive release of the inhibitory amino acid taurine in ischemic conditions could act neuroprotectively, counteracting in several ways the effects of simultaneous release of excitatory amino acids. This protection could be of great importance in developing brain tissue, while also having an effect in aged brains.

  16. Stochastic modeling of oligodendrocyte generation in cell culture: model validation with time-lapse data

    Directory of Open Access Journals (Sweden)

    Noble Mark

    2006-05-01

    Full Text Available Abstract Background The purpose of this paper is two-fold. The first objective is to validate the assumptions behind a stochastic model developed earlier by these authors to describe oligodendrocyte generation in cell culture. The second is to generate time-lapse data that may help biomathematicians to build stochastic models of cell proliferation and differentiation under other experimental scenarios. Results Using time-lapse video recording it is possible to follow the individual evolutions of different cells within each clone. This experimental technique is very laborious and cannot replace model-based quantitative inference from clonal data. However, it is unrivalled in validating the structure of a stochastic model intended to describe cell proliferation and differentiation at the clonal level. In this paper, such data are reported and analyzed for oligodendrocyte precursor cells cultured in vitro. Conclusion The results strongly support the validity of the most basic assumptions underpinning the previously proposed model of oligodendrocyte development in cell culture. However, there are some discrepancies; the most important is that the contribution of progenitor cell death to cell kinetics in this experimental system has been underestimated.

  17. Two-way coupling of magnetohydrodynamic simulations with embedded particle-in-cell simulations

    Science.gov (United States)

    Makwana, K. D.; Keppens, R.; Lapenta, G.

    2017-12-01

    We describe a method for coupling an embedded domain in a magnetohydrodynamic (MHD) simulation with a particle-in-cell (PIC) method. In this two-way coupling we follow the work of Daldorff et al. (2014) [19] in which the PIC domain receives its initial and boundary conditions from MHD variables (MHD to PIC coupling) while the MHD simulation is updated based on the PIC variables (PIC to MHD coupling). This method can be useful for simulating large plasma systems, where kinetic effects captured by particle-in-cell simulations are localized but affect global dynamics. We describe the numerical implementation of this coupling, its time-stepping algorithm, and its parallelization strategy, emphasizing the novel aspects of it. We test the stability and energy/momentum conservation of this method by simulating a steady-state plasma. We test the dynamics of this coupling by propagating plasma waves through the embedded PIC domain. Coupling with MHD shows satisfactory results for the fast magnetosonic wave, but significant distortion for the circularly polarized Alfvén wave. Coupling with Hall-MHD shows excellent coupling for the whistler wave. We also apply this methodology to simulate a Geospace Environmental Modeling (GEM) challenge type of reconnection with the diffusion region simulated by PIC coupled to larger scales with MHD and Hall-MHD. In both these cases we see the expected signatures of kinetic reconnection in the PIC domain, implying that this method can be used for reconnection studies.

  18. Defined α-synuclein prion-like molecular assemblies spreading in cell culture.

    Science.gov (United States)

    Aulić, Suzana; Le, Tran Thanh Nhat; Moda, Fabio; Abounit, Saïda; Corvaglia, Stefania; Casalis, Loredana; Gustincich, Stefano; Zurzolo, Chiara; Tagliavini, Fabrizio; Legname, Giuseppe

    2014-06-04

    α-Synuclein (α-syn) plays a central role in the pathogenesis of synucleinopathies, a group of neurodegenerative disorders that includes Parkinson disease, dementia with Lewy bodies and multiple system atrophy. Several findings from cell culture and mouse experiments suggest intercellular α-syn transfer. Through a methodology used to obtain synthetic mammalian prions, we tested whether recombinant human α-syn amyloids can promote prion-like accumulation in neuronal cell lines in vitro. A single exposure to amyloid fibrils of human α-syn was sufficient to induce aggregation of endogenous α-syn in human neuroblastoma SH-SY5Y cells. Remarkably, endogenous wild-type α-syn was sufficient for the formation of these aggregates, and overexpression of the protein was not required. Our results provide compelling evidence that endogenous α-syn can accumulate in cell culture after a single exposure to exogenous α-syn short amyloid fibrils. Importantly, using α-syn short amyloid fibrils as seed, endogenous α-syn aggregates and accumulates over several passages in cell culture, providing an excellent tool for potential therapeutic screening of pathogenic α-syn aggregates.

  19. REMOTE INTERVENTION TOWER ELIMINATION SYSTEM

    International Nuclear Information System (INIS)

    Dave Murnane; Renauld Washington

    2002-01-01

    This Topical Report is presented to satisfy reporting requirements in the Statement of work section J.5 page 120 per Department of Energy contract DE-AC26-01NT41093. The project does not contain any imperial research data. This report describes the assembly of Commercial off the shelf (COTS) items configured in a unique manner to represent new and innovative technology in the service of size reduction and material handling at DOE sites, to assist in the DandD effort currently underway at the designated DOE Facilities

  20. Multi-sensors multi-baseline mapping system for mobile robot using stereovision camera and laser-range device

    Directory of Open Access Journals (Sweden)

    Mohammed Faisal

    2016-06-01

    Full Text Available Countless applications today are using mobile robots, including autonomous navigation, security patrolling, housework, search-and-rescue operations, material handling, manufacturing, and automated transportation systems. Regardless of the application, a mobile robot must use a robust autonomous navigation system. Autonomous navigation remains one of the primary challenges in the mobile-robot industry; many control algorithms and techniques have been recently developed that aim to overcome this challenge. Among autonomous navigation methods, vision-based systems have been growing in recent years due to rapid gains in computational power and the reliability of visual sensors. The primary focus of research into vision-based navigation is to allow a mobile robot to navigate in an unstructured environment without collision. In recent years, several researchers have looked at methods for setting up autonomous mobile robots for navigational tasks. Among these methods, stereovision-based navigation is a promising approach for reliable and efficient navigation. In this article, we create and develop a novel mapping system for a robust autonomous navigation system. The main contribution of this article is the fuse of the multi-baseline stereovision (narrow and wide baselines and laser-range reading data to enhance the accuracy of the point cloud, to reduce the ambiguity of correspondence matching, and to extend the field of view of the proposed mapping system to 180°. Another contribution is the pruning the region of interest of the three-dimensional point clouds to reduce the computational burden involved in the stereo process. Therefore, we called the proposed system multi-sensors multi-baseline mapping system. The experimental results illustrate the robustness and accuracy of the proposed system.

  1. Testing of serum atherogenicity in cell cultures: questionable data published

    Directory of Open Access Journals (Sweden)

    Sergei V. Jargin

    2012-01-01

    Full Text Available In a large series of studies was reported that culturing of smooth muscle cells with serum from atherosclerosis patients caused intracellular lipid accumulation, while serum from healthy controls had no such effect. Cultures were used for evaluation of antiatherogenic drugs. Numerous substances were reported to lower serum atherogenicity: statins, trapidil, calcium antagonists, garlic derivatives etc. On the contrary, beta-blockers, phenothiazines and oral hypoglycemics were reported to be pro-atherogenic. Known antiatherogenic agents can influence lipid metabolism and cholesterol synthesis, intestinal absorption or endothelium-related mechanisms. All these targets are absent in cell monocultures. Inflammatory factors, addressed by some antiatherogenic drugs, are also not reproduced. In vivo, relationship between cholesterol uptake by cells and atherogenesis must be inverse rather than direct: in familial hypercholesterolemia, inefficient clearance of LDL-cholesterol by cells predisposes to atherosclerosis. Accordingly, if a pharmacological agent reduces cholesterol uptake by cells in vitro, it should be expected to elevate cholesterol in vivo. Validity of clinical recommendations, based on serum atherogenicity testing in cell monocultures, is therefore questionable. These considerations pertain also to the drugs developed on the basis of the cell culture experiments.

  2. Efficacy of decoquinate against Sarcocystis neurona in cell cultures.

    Science.gov (United States)

    Lindsay, David S; Nazir, M Mudasser; Maqbool, Azhar; Ellison, Siobhan P; Strobl, Jeannine S

    2013-09-01

    Decoquinate is a quinolone anticoccidial approved for use in the prevention of intestinal coccidiosis in farm animals. This compound has good activity against Toxoplasma gondii and Neospora caninum in cell cultures. The drug acts on the parasites' mitochondria. The activity of decoquinate against developing merozoites of 2 isolates of Sarcocystis neurona was examined in cell culture. Merozoite production at 10 days was completely inhibited when decoquinate was used at 20 or 240 nM. The IC50 of decoquinate was 0.5 ± 0.09nM for the Sn6 isolate of S. neurona from a horse and 1.1 ± 0.6 nM for the SnOP15 isolate of S. neurona from an opossum. Levamisole was toxic at 5 μg/ml and no synergism was observed when decoquinate was combined with levamisole and tested against the Sn3YFP isolate of S. neurona. Decoquinate was cidal for developing schizonts of S. neurona at 240 nM. Copyright © 2013 Elsevier B.V. All rights reserved.

  3. Applying image quality in cell phone cameras: lens distortion

    Science.gov (United States)

    Baxter, Donald; Goma, Sergio R.; Aleksic, Milivoje

    2009-01-01

    This paper describes the framework used in one of the pilot studies run under the I3A CPIQ initiative to quantify overall image quality in cell-phone cameras. The framework is based on a multivariate formalism which tries to predict overall image quality from individual image quality attributes and was validated in a CPIQ pilot program. The pilot study focuses on image quality distortions introduced in the optical path of a cell-phone camera, which may or may not be corrected in the image processing path. The assumption is that the captured image used is JPEG compressed and the cellphone camera is set to 'auto' mode. As the used framework requires that the individual attributes to be relatively perceptually orthogonal, in the pilot study, the attributes used are lens geometric distortion (LGD) and lateral chromatic aberrations (LCA). The goal of this paper is to present the framework of this pilot project starting with the definition of the individual attributes, up to their quantification in JNDs of quality, a requirement of the multivariate formalism, therefore both objective and subjective evaluations were used. A major distinction in the objective part from the 'DSC imaging world' is that the LCA/LGD distortions found in cell-phone cameras, rarely exhibit radial behavior, therefore a radial mapping/modeling cannot be used in this case.

  4. In-cell overlay metrology by using optical metrology tool

    Science.gov (United States)

    Lee, Honggoo; Han, Sangjun; Hong, Minhyung; Kim, Seungyoung; Lee, Jieun; Lee, DongYoung; Oh, Eungryong; Choi, Ahlin; Park, Hyowon; Liang, Waley; Choi, DongSub; Kim, Nakyoon; Lee, Jeongpyo; Pandev, Stilian; Jeon, Sanghuck; Robinson, John C.

    2018-03-01

    Overlay is one of the most critical process control steps of semiconductor manufacturing technology. A typical advanced scheme includes an overlay feedback loop based on after litho optical imaging overlay metrology on scribeline targets. The after litho control loop typically involves high frequency sampling: every lot or nearly every lot. An after etch overlay metrology step is often included, at a lower sampling frequency, in order to characterize and compensate for bias. The after etch metrology step often involves CD-SEM metrology, in this case in-cell and ondevice. This work explores an alternative approach using spectroscopic ellipsometry (SE) metrology and a machine learning analysis technique. Advanced 1x nm DRAM wafers were prepared, including both nominal (POR) wafers with mean overlay offsets, as well as DOE wafers with intentional across wafer overlay modulation. After litho metrology was measured using optical imaging metrology, as well as after etch metrology using both SE and CD-SEM for comparison. We investigate 2 types of machine learning techniques with SE data: model-less and model-based, showing excellent performance for after etch in-cell on-device overlay metrology.

  5. Deploying electromagnetic particle-in-cell (EM-PIC) codes on Xeon Phi accelerators boards

    Science.gov (United States)

    Fonseca, Ricardo

    2014-10-01

    The complexity of the phenomena involved in several relevant plasma physics scenarios, where highly nonlinear and kinetic processes dominate, makes purely theoretical descriptions impossible. Further understanding of these scenarios requires detailed numerical modeling, but fully relativistic particle-in-cell codes such as OSIRIS are computationally intensive. The quest towards Exaflop computer systems has lead to the development of HPC systems based on add-on accelerator cards, such as GPGPUs and more recently the Xeon Phi accelerators that power the current number 1 system in the world. These cards, also referred to as Intel Many Integrated Core Architecture (MIC) offer peak theoretical performances of >1 TFlop/s for general purpose calculations in a single board, and are receiving significant attention as an attractive alternative to CPUs for plasma modeling. In this work we report on our efforts towards the deployment of an EM-PIC code on a Xeon Phi architecture system. We will focus on the parallelization and vectorization strategies followed, and present a detailed performance evaluation of code performance in comparison with the CPU code.

  6. Experience of in-cell visual inspection using CCD camera in hot cell of Reprocessing Plant

    International Nuclear Information System (INIS)

    Reddy, Padi Srinivas; Amudhu Ramesh Kumar, R.; Geo Mathews, M.; Ravisankar, A.

    2013-01-01

    This paper describes the selection, customization and operating experience of the visual inspection system for the hot cell of a Reprocessing Plant. For process equipment such as fuel chopping machine, dissolver, centrifuge, centrifugal extractors etc., viewing of operations and maintenance using manipulators is required. For this, the service of in-cell camera is essential. The ambience of the hot cell of Compact facility for Reprocessing of Advanced fuels in Lead cell (CORAL) for the reprocessing of fast reactor spent fuel has high gamma radiation and acidic vapors. Black and white Charge Coupled Device (CCD) camera has been used in CORAL incorporating in-house modifications to suit the operating ambient conditions, thereby extending the operating life of the camera. (author)

  7. Particle-in-cell plasma simulations of the modified two-stream instability

    Directory of Open Access Journals (Sweden)

    K. Schlegel

    1994-08-01

    Full Text Available We model the modified two-stream plasma instability occurring in the ionospheric E-region using a 2.5-dimensional particle-in-cell code. Compared to previous similar work we concentrate on simulated quantities that can easily be measured in the real ionosphere by coherent radars or rockets, such as the Doppler velocity, the backscattered power, backscattered spectra, aspect angle behaviour and electron temperature enhancement. Despite using a relatively small simulation model, we obtain remarkably good agreement between actual observed and simulated plasma parameters. The advantage of such a small system is that we were able to perform (other than in previous related work many simulation runs with different sets of input parameters, thus studying the unstable plasma under various conditions.

  8. At the cutting edge: applications and perspectives of laser nanosurgery in cell biology.

    Science.gov (United States)

    Ronchi, Paolo; Terjung, Stefan; Pepperkok, Rainer

    2012-04-01

    Laser-mediated nanosurgery has become popular in the last decade because of the previously unexplored possibility of ablating biological material inside living cells with sub-micrometer precision. A number of publications have shown the potential applications of this technique, ranging from the dissection of sub-cellular structures to surgical ablations of whole cells or tissues in model systems such as Drosophila melanogaster or Danio rerio . In parallel, the recent development of micropatterning techniques has given cell biologists the possibility to shape cells and reproducibly organize the intracellular space. The integration of these two techniques has only recently started yet their combination has proven to be very interesting. The aim of this review is to present recent applications of laser nanosurgery in cell biology and to discuss the possible developments of this approach, particularly in combination with micropattern-mediated endomembrane organization.

  9. Coupling biochemistry and mechanics in cell adhesion: a model for inhomogeneous stress fiber contraction

    International Nuclear Information System (INIS)

    Besser, Achim; Schwarz, Ulrich S

    2007-01-01

    Biochemistry and mechanics are closely coupled in cell adhesion. At sites of cell-matrix adhesion, mechanical force triggers signaling through the Rho-pathway, which leads to structural reinforcement and increased contractility in the actin cytoskeleton. The resulting force acts back to the sites of adhesion, resulting in a positive feedback loop for mature adhesion. Here, we model this biochemical-mechanical feedback loop for the special case when the actin cytoskeleton is organized in stress fibers, which are contractile bundles of actin filaments. Activation of myosin II molecular motors through the Rho-pathway is described by a system of reaction-diffusion equations, which are coupled into a viscoelastic model for a contractile actin bundle. We find strong spatial gradients in the activation of contractility and in the corresponding deformation pattern of the stress fiber, in good agreement with experimental findings

  10. The use of electromagnetic particle-in-cell codes in accelerator applications

    International Nuclear Information System (INIS)

    Eppley, K.

    1988-12-01

    The techniques developed for the numerical simulation of plasmas have numerous applications relevant to accelerators. The operation of many accelerator components involves transients, interactions between beams and rf fields, and internal plasma oscillations. These effects produce non-linear behavior which can be represented accurately by particle in cell (PIC) simulations. We will give a very brief overview of the algorithms used in PIC Codes. We will examine the range of parameters over which they are useful. We will discuss the factors which determine whether a two or three dimensional simulation is most appropriate. PIC codes have been applied to a wide variety of diverse problems, spanning many of the systems in a linear accelerator. We will present a number of practical examples of the application of these codes to areas such as guns, bunchers, rf sources, beam transport, emittance growth and final focus. 8 refs., 8 figs., 2 tabs

  11. Mass spectrometry based proteomics in cell biology and signaling research

    International Nuclear Information System (INIS)

    Mann, M.; Andersen, J.; Ishihama, Y.; Rappsilber, J.; Ong, S.; Foster, L.; Blagoev, B.; Kratchmarova, I.; Lasonder, E.

    2002-01-01

    Full text: Proteomics is one of the most powerful post-genomics technologies. Recently accomplishments include large scale protein-protein interaction mapping, large scale mapping of phosphorylation sites and the cloning of key signaling molecules. In this talk, current state of the art of the technology will be reviewed. Applications of proteomics to the mapping of multiprotein complexes will be illustrated with recent work on the spliceosome and the nucleolus. More than 300 proteins have been mapped to each of these complexes. Quantitative techniques are becoming more and more essential in proteomics. They are usually performed by the incorporation of stable isotopes - a light form in cell state 'A' and a heavy form in cell state 'E' - and subsequent comparison of mass spectrometric peak heights. A new technique called, SILAC for Stable isotope Incorporation by Amino acids in Cell culture, has been applied to studying cell differentiation and mapping secreted proteins from adipocytes. A number of known and novel proteins important in adipocyte differentiation have been identified by this technique. Some of these proved to be upregulated at the 1 mRNA level, too, whereas others appear to be regulated post-translationally. We have also applied the SILAC method to protein-protein interaction mapping. For example, we compared immunoprecipitates from stimulated and non-stimulated cells to find binding partners recruited to the bait due to the stimulus. Several novel substrates in the EGF pathway were found in this way. An important application of proteomics in the signaling field is the mapping of post-translational modifications. In particular, there are a number of techniques for phosphotyrosine phosphorylation mapping which have proven very useful. Making use of the mass deficiency of the phosphogroup, 'parent ion scans' con be performed, which selectively reveal phosphotyrosine peptides from complex peptides mixtures. This technique has been used to clone several

  12. A BACTERIA FORAGING ALGORITHM FOR SOLVING INTEGRATED MULTI-PERIOD CELL FORMATION AND SUBCONTRACTING PRODUCTION PLANNING IN A DYNAMIC CELLULAR MANUFACTURING SYSTEM

    Directory of Open Access Journals (Sweden)

    S.H. Tang

    2012-01-01

    Full Text Available

    ENGLISH ABSTRACT: The bacteria foraging algorithm (BFA is a new computation technique inspired by the social foraging behaviour of Escherichia coli (E. coli bacteria. Since the introduction of the BFA by Kevin M. Passino, there have been many challenges in employing this algorithm to problems other than those for which the algorithm was proposed. This research aims to apply this emerging optimisation algorithm to develop a mixed-integer programming model for designing cellular manufacturing systems (CMSs, and production planning in dynamic environments. In dynamic environments, product mix and part demand vary under multi-period planning horizons. Thus the best-designed cells for one period may not be adequate for subsequent periods, requiring their reconstruction. The advantages of the proposed model are as follows: consideration of batch inter-cell and intra-cell material handling by assuming the sequence of operations, allowing for alternative process plans for part types, and consideration of machine copying, with an emphasis on the effect of trade-offs between production and outsourcing costs. The goal is to minimise the sum of the machines’ constant and variable costs, inter-cell and intra-cell material handling costs, reconstruction costs, partial subcontracting costs, and inventory carrying costs. In addition, a newly-developed BFA-based optimisation algorithm has been compared with the branch and bound algorithm. The results suggest that the proposed algorithm performs better than related works.

    AFRIKAANSE OPSOMMING: Die ‘bacteria foraging algorithm’ (BFA is ‘n berekeningstegniek gebaseeer op die sosiale soekgedrag van Escherichia coli (E. coli bakterieë. Sedert die bekendstelling van BFA was daar talle uitdagings oor toepassings van die algoritme op ander probleme as dié waarvoor dit ontwikkel is. Dié navorsing poog om deur toepassing van die algoritme ‘n gemengde heelgetalprogrammeringmodel te ontwikkel vir die

  13. Somatic embryogenesis in cell cultures of Glycine species.

    Science.gov (United States)

    Gamborg, O L; Davis, B P; Stahlhut, R W

    1983-08-01

    This report describes the development of procedures for the production of somatic embryos in cell cultures of Glycine species including soybean. The conditions for callus induction and initiation of rapidly growing cell suspension cultures were defined. Methods for inducing embryogenesis were tested on 16 lines of several Glycine species and cultivars of soybean. The SB-26 Culture of a G. soja gave the best results and was used in the experiments. Embryogenesis required the presence of picloram or 2,4-D. AMO 1618, CCC, PP-333 and Ancymidol enhanced the embryogenesis frequency. Plants of the G. soja (SB-26) were grown to maturity from seed-derived shoot tips. Characteristics of the plants are discussed.

  14. KCNQ1 channels sense small changes in cell volume

    DEFF Research Database (Denmark)

    Grunnet, Morten; Jespersen, Thomas; MacAulay, Nanna

    2003-01-01

    Many important physiological processes involve changes in cell volume, e.g. the transport of salt and water in epithelial cells and the contraction of cardiomyocytes. In this study, we show that voltage-gated KCNQ1 channels, which are strongly expressed in epithelial cells or cardiomyocytes......, and KCNQ4 channels, expressed in hair cells and the auditory tract, are tightly regulated by small cell volume changes when co-expressed with aquaporin 1 water-channels (AQP1) in Xenopus oocytes. The KCNQ1 and KCNQ4 current amplitudes precisely reflect the volume of the oocytes. By contrast, the related...... KCNQ2 and KCNQ3 channels, which are prominently expressed in neurons, are insensitive to cell volume changes. The sensitivity of the KCNQ1 and KCNQ4 channels to cell volume changes is independent of the presence of the auxiliary KCNE1-3 subunits, although modulated by KCNE1 in the case of KCNQ1...

  15. The Particle-in-Cell and Kinetic Simulation Software Center

    Science.gov (United States)

    Mori, W. B.; Decyk, V. K.; Tableman, A.; Fonseca, R. A.; Tsung, F. S.; Hu, Q.; Winjum, B. J.; An, W.; Dalichaouch, T. N.; Davidson, A.; Hildebrand, L.; Joglekar, A.; May, J.; Miller, K.; Touati, M.; Xu, X. L.

    2017-10-01

    The UCLA Particle-in-Cell and Kinetic Simulation Software Center (PICKSC) aims to support an international community of PIC and plasma kinetic software developers, users, and educators; to increase the use of this software for accelerating the rate of scientific discovery; and to be a repository of knowledge and history for PIC. We discuss progress towards making available and documenting illustrative open-source software programs and distinct production programs; developing and comparing different PIC algorithms; coordinating the development of resources for the educational use of kinetic software; and the outcomes of our first sponsored OSIRIS users workshop. We also welcome input and discussion from anyone interested in using or developing kinetic software, in obtaining access to our codes, in collaborating, in sharing their own software, or in commenting on how PICKSC can better serve the DPP community. Supported by NSF under Grant ACI-1339893 and by the UCLA Institute for Digital Research and Education.

  16. Wavenumber spectrum of whistler turbulence: Particle-in-cell simulation

    International Nuclear Information System (INIS)

    Saito, S.; Gary, S. Peter; Narita, Y.

    2010-01-01

    The forward cascade of decaying whistler turbulence is studied in low beta plasma to understand essential properties of the energy spectrum at electron scales, by using a two-dimensional electromagnetic particle-in-cell (PIC) simulation. This simulation demonstrates turbulence in which the energy cascade rate is greater than the dissipation rate at the electron inertial length. The PIC simulation shows that the magnetic energy spectrum of forward-cascaded whistler turbulence at electron inertial scales is anisotropic and develops a very steep power-law spectrum which is consistent with recent solar wind observations. A comparison of the simulated spectrum with that predicted by a phenomenological turbulence scaling model suggests that the energy cascade at the electron inertial scale depends on both magnetic fluctuations and electron velocity fluctuations, as well as on the whistler dispersion relation. Thus, not only kinetic Alfven turbulence but also whistler turbulence may explain recent solar wind observations of very steep magnetic spectra at short scales.

  17. Hsp90 inhibitors reduce influenza virus replication in cell culture

    International Nuclear Information System (INIS)

    Chase, Geoffrey; Deng, Tao; Fodor, Ervin; Leung, B.W.; Mayer, Daniel; Schwemmle, Martin; Brownlee, George

    2008-01-01

    The viral RNA polymerase complex of influenza A virus consists of three subunits PB1, PB2 and PA. Recently, the cellular chaperone Hsp90 was shown to play a role in nuclear import and assembly of the trimeric polymerase complex by binding to PB1 and PB2. Here we show that Hsp90 inhibitors, geldanamycin or its derivative 17-AAG, delay the growth of influenza virus in cell culture resulting in a 1-2 log reduction in viral titre early in infection. We suggest that this is caused by the reduced half-life of PB1 and PB2 and inhibition of nuclear import of PB1 and PA which lead to reduction in viral RNP assembly. Hsp90 inhibitors may represent a new class of antiviral compounds against influenza viruses

  18. Small molecule alteration of RNA sequence in cells and animals.

    Science.gov (United States)

    Guan, Lirui; Luo, Yiling; Ja, William W; Disney, Matthew D

    2017-10-18

    RNA regulation and maintenance are critical for proper cell function. Small molecules that specifically alter RNA sequence would be exceptionally useful as probes of RNA structure and function or as potential therapeutics. Here, we demonstrate a photochemical approach for altering the trinucleotide expanded repeat causative of myotonic muscular dystrophy type 1 (DM1), r(CUG) exp . The small molecule, 2H-4-Ru, binds to r(CUG) exp and converts guanosine residues to 8-oxo-7,8-dihydroguanosine upon photochemical irradiation. We demonstrate targeted modification upon irradiation in cell culture and in Drosophila larvae provided a diet containing 2H-4-Ru. Our results highlight a general chemical biology approach for altering RNA sequence in vivo by using small molecules and photochemistry. Furthermore, these studies show that addition of 8-oxo-G lesions into RNA 3' untranslated regions does not affect its steady state levels. Copyright © 2017 Elsevier Ltd. All rights reserved.

  19. Propagation and isolation of ranaviruses in cell culture

    DEFF Research Database (Denmark)

    Ariel, Ellen; Nicolajsen, Nicole; Christophersen, Maj-Britt

    2009-01-01

    The optimal in vitro propagation procedure for a panel of ranavirus isolates and the best method for isolation of Epizootic haematopoietic necrosis virus (EHNV) from organ material in cell-culture were investigated. The panel of ranavirus isolates included: Frog virus 3 (FV3), Bohle iridovirus (BIV......), Pike-perch iridovirus (PPIV), European catfish virus (ECV), European sheatfish virus (ESV), EHNV, Doctor fish virus (DFV), Guppy virus 6 (GF6), short-finned eel virus (SERV) and Rana esculenta virus Italy 282/102 (REV 282/102). Each isolate was titrated in five cell lines: bluegill fry (BF-2......), epithelioma papulosum cyprini (EPC), chinook salmon embryo (CHSE-214) rainbow trout gonad (RTG-2) and fathead minnow (FHM), and incubated at 10, 15, 20, 24 and 28 °C for two weeks. BF-2, EPC and CHSE-214 cells performed well and titers obtained in the three cell lines were similar, whereas FHM and RTG-2 cells...

  20. Direct determination of phosphatase activity from physiological substrates in cells.

    Directory of Open Access Journals (Sweden)

    Zhongyuan Ren

    Full Text Available A direct and continuous approach to determine simultaneously protein and phosphate concentrations in cells and kinetics of phosphate release from physiological substrates by cells without any labeling has been developed. Among the enzymes having a phosphatase activity, tissue non-specific alkaline phosphatase (TNAP performs indispensable, multiple functions in humans. It is expressed in numerous tissues with high levels detected in bones, liver and neurons. It is absolutely required for bone mineralization and also necessary for neurotransmitter synthesis. We provided the proof of concept that infrared spectroscopy is a reliable assay to determine a phosphatase activity in the osteoblasts. For the first time, an overall specific phosphatase activity in cells was determined in a single step by measuring simultaneously protein and substrate concentrations. We found specific activities in osteoblast like cells amounting to 116 ± 13 nmol min(-1 mg(-1 for PPi, to 56 ± 11 nmol min(-1 mg(-1 for AMP, to 79 ± 23 nmol min(-1 mg(-1 for beta-glycerophosphate and to 73 ± 15 nmol min(-1 mg(-1 for 1-alpha-D glucose phosphate. The assay was also effective to monitor phosphatase activity in primary osteoblasts and in matrix vesicles. The use of levamisole--a TNAP inhibitor--served to demonstrate that a part of the phosphatase activity originated from this enzyme. An IC50 value of 1.16 ± 0.03 mM was obtained for the inhibition of phosphatase activity of levamisole in osteoblast like cells. The infrared assay could be extended to determine any type of phosphatase activity in other cells. It may serve as a metabolomic tool to monitor an overall phosphatase activity including acid phosphatases or other related enzymes.

  1. Gelatin methacrylamide as coating material in cell culture.

    Science.gov (United States)

    Egger, Michael; Tovar, Günter E M; Hoch, Eva; Southan, Alexander

    2016-06-13

    Unmodified gelatin (uG) is widely used as a coating material in cell culture for improving surface properties. In this study, the authors investigated if gelatin methacrylamide (GM) with a medium degree of methacrylamide modification (GM1.5) and a high degree of methacrylamide modification (GM4) are equally suitable for this purpose. Therefore, gold surfaces were coated with uG, GM1.5, and GM4 by adsorption of the polymers on the surfaces. Coating success was confirmed by spectroscopic ellipsometry, contact angle measurements, surface plasmon resonance spectroscopy (SPRS), and atomic force microscopy (AFM). The authors found that upon adsorption of uG, GM1.5, a nd GM4 on gold, thin films with thicknesses of 2.95 nm, 2.50 nm, and 2.26 nm were formed. The coated surfaces showed advancing contact angles of 46° (uG and GM1.5) and 52° (GM4) without alteration of the surface roughness determined by AFM. Protein adsorption taking place on the coated surfaces was measured during contact of the surfaces with fetal calf serum by SPRS. Protein adsorption on the coated surfaces was reduced by the factor of 6.4 (uG), 5.4 (GM1.5), and 4.6 (GM4) compared to gold surfaces. Human fibroblasts cultured on the surfaces showed excellent viability shown by water soluble tetrazolium salt assay as well as live/dead staining with propidium iodide and fluorescein diacetate. No cytotoxic effects of the GM coated surfaces were observed, giving rise to the conclusion that GMs are suitable materials as coatings in cell culture.

  2. Primary radiation damage and disturbance in cell divisions

    International Nuclear Information System (INIS)

    Kim, Jin Kyu; Lee, Yun-Jong; Kim, Jae-Hun; Petin, Vladislav G.; Nili, Mohammad

    2008-01-01

    Survived cells from a homogeneous population exposed to ionizing radiation form various colonies of different sizes and morphology on a solid nutrient medium, which appear at different time intervals after irradiation. Such a phenomenon agrees well with the modern theory of microdosimetry and classical hit-and-target models of radiobiology. According to the hit-principle, individual cells exposed to the same dose of radiation are damaged in different manners. It means that the survived cells can differ in the content of sublethal damage (hits) produced by the energy absorbed into the cell and which is not enough to give rise to effective radiation damage which is responsible for cell killing or inactivation. In diploid yeast cells, the growth rate of cells from 250 colonies of various sizes appeared at different time intervals after irradiation with 600 Gy of gamma radiation from a 60 Co isotopic source was analyzed. The survival rate after irradiation was 20%. Based on the analyses results, it was possible to categorize the clones grown from irradiated cells according to the number of sub-lesions from 1 to 4. The clones with various numbers of sub-lesions were shown to be different in their viability, radiosensitivity, sensitivity to environmental conditions, and the frequency of recombination and respiratory deficient mutations. Cells from unstable clones exhibited an enhanced radiosensitivity, and an increased portion of morphologically changed cells, nonviable cells and respiration mutants, as well. The degree of expression of the foregoing effects was higher if the number of primary sublethal lesions was greater in the originally irradiated cell. Disturbance in cell division can be characterized by cell inactivation or incorrect distribution of mitochondria between daughter cells. Thus, the suggested methodology of identification of cells with a definite number of primary sublethal lesions will promote further elucidation of the nature of primary radiation

  3. Molecular imaging of lipids in cells and tissues

    Science.gov (United States)

    Borner, Katrin; Malmberg, Per; Mansson, Jan-Eric; Nygren, Hakan

    2007-02-01

    The distribution pattern of lipid species in biological tissues was analyzed with imaging mass spectrometry (TOF-SIMS; time-of-flight secondary ion mass spectrometry). The first application shows distribution of a glycosphingolipid, the galactosylceramide-sulfate (sulfatide) with different hydrocarbon chain lengths and the fatty acids palmitate and oleate in rat cerebellum. Sulfatides were seen localized in regions suggested as paranodal areas of rat cerebellar white matter as well as in the granular layer, with highest concentrations at the borders of the white matter. Different distribution patterns could be shown for the fatty acid C16:0 palmitate and C18:1 oleate in rat cerebellum, which seem to origin partly from the hydrocarbon chains of phosphatidylcholine. Results were shown for two different tissue preparation methods, which were plunge-freezing and cryostat sectioning as well as high-pressure freezing, freeze-fracturing and freeze-drying. The second application shows TOF-SIMS analysis on a biological trial of choleratoxin treatment in mouse intestine. The effect of cholera toxin on lipids in the intestinal epithelium was shown by comparing control and cholera toxin treated mouse intestine samples. A significant increase of the cholesterol concentration was seen after treatment. Cholesterol was mainly localized to the brush border of enterocytes of the intestinal villi, which could be explained by the presence of cholesterol-rich lipid rafts present on the microvilli or by relations to cholesterol uptake. After cholera toxin exposure, cholesterol was seen increased in the nuclei of enterocytes and apparently in the interstitium of the villi. We find that imaging TOF-SIMS is a powerful tool for studies of lipid distributions in cells and tissues, enabling the elucidation of their role in cell function and biology.

  4. Changes in cell adhesion molecule expression on T cells associated with systemic virus infection

    DEFF Research Database (Denmark)

    Andersson, E C; Christensen, Jan Pravsgaard; Marker, O

    1994-01-01

    -4, LFA-1, and ICAM-1, are up-regulated on CD8+ cells, whereas the lymph node homing receptor MEL-14 is down-regulated during the infection; only marginal changes were observed for CD4+ cells. Basically similar but less marked results were obtained in mice infected with Pichinde virus. Further...

  5. Cholesterol inhibits entotic cell-in-cell formation and actomyosin contraction.

    Science.gov (United States)

    Ruan, Banzhan; Zhang, Bo; Chen, Ang; Yuan, Long; Liang, Jianqing; Wang, Manna; Zhang, Zhengrong; Fan, Jie; Yu, Xiaochen; Zhang, Xin; Niu, Zubiao; Zheng, You; Gu, Songzhi; Liu, Xiaoqing; Du, Hongli; Wang, Jufang; Hu, Xianwen; Gao, Lihua; Chen, Zhaolie; Huang, Hongyan; Wang, Xiaoning; Sun, Qiang

    2018-01-01

    Cell-in-cell structure is prevalent in human cancer, and associated with several specific pathophysiological phenomena. Although cell membrane adhesion molecules were found critical for cell-in-cell formation, the roles of other membrane components, such as lipids, remain to be explored. In this study, we attempted to investigate the effects of cholesterol and phospholipids on the formation of cell-in-cell structures by utilizing liposome as a vector. We found that Lipofectamine-2000, the reagent commonly used for routine transfection, could significantly reduce entotic cell-in-cell formation in a cell-specific manner, which is correlated with suppressed actomyosin contraction as indicated by reduced β-actin expression and myosin light chain phosphorylation. The influence on cell-in-cell formation was likely dictated by specific liposome components as some liposomes affected cell-in-cell formation while some others didn't. Screening on a limited number of lipids, the major components of liposome, identified phosphatidylethanolamine (PE), stearamide (SA), lysophosphatidic acid (LPA) and cholesterol (CHOL) as the inhibitors of cell-in-cell formation. Importantly, cholesterol treatment significantly inhibited myosin light chain phosphorylation, which resembles the effect of Lipofectamine-2000, suggesting cholesterol might be partially responsible for liposomes' effects on cell-in-cell formation. Together, our findings supporting a role of membrane lipids and cholesterol in cell-in-cell formation probably via regulating actomyosin contraction. Copyright © 2017 Elsevier Inc. All rights reserved.

  6. LANL operating experience with the WAND and HERCULES prototype systems

    International Nuclear Information System (INIS)

    Gruetzmacher, K. M.; Foxx, C. L.; MYERS, S. C.

    2000-01-01

    The Waste Assay for Nonradioactive Disposal (WAND) and the High Efficiency Radiation Counters for Ultimate Low Emission Sensitivity (HERCULES) prototype systems have been operating at Los Alamos National Laboratory's (LANL's) Solid Waste Operation's (SWO'S) non-destructive assay (NDA) building since 1997 and 1998, respectively. These systems are the cornerstone of the verification program for low-density Green is Clean (GIC) waste at the Laboratory. GIC waste includes all non-regulated waste generated in radiological controlled areas (RCAS) that has been actively segregated as clean (i.e., nonradioactive) through the use of waste generator acceptable knowledge (AK). The use of this methodology alters LANL's past practice of disposing of all room trash generated in nuclear facilities in radioactive waste landfills. Waste that is verified clean can be disposed of at the Los Alamos County Landfill. It is estimated that 50-90% of the low-density room trash from radioactive material handling areas at Los Alamos might be free of contamination. This approach avoids the high cost of disposal of clean waste at a radioactive waste landfill. It also reduces consumption of precious space in the radioactive waste landfill where disposal of this waste provides no benefit to the public or the environment. Preserving low level waste (LLW) disposal capacity for truly radioactive waste is critical in this era when expanding existing radioactive waste landfills or permitting new ones is resisted by regulators and stakeholders. This paper describes the operating experience with the WAND and HERCULES since they began operation at SWO. Waste for verification by the WAND system has been limited so far to waste from the Plutonium Facility and the Solid Waste Operations Facility. A total of461 ft3 (13.1 m3) of low-density shredded waste and paper have been verified clean by the WAND system. The HERCULES system has been used to verify waste from four Laboratory facilities. These are the

  7. A software platform to develop and execute kitting tasks on industrial cyber-physical systems

    DEFF Research Database (Denmark)

    Rovida, Francesco

    2017-01-01

    The current material handling infrastructure associated with manufacturing and assembly operations still register a great presence of human work for highly repetitive tasks. A major contributing factor for the low automation is that current manufacturing robots have little or no understanding of ....... A platform where skills, similarly to computer or smartphone applications, can be installed and removed from heterogeneous robots with few elementary steps....

  8. Scheduling System for Multiple Unmanned Aerial Vehicles in Indoor Environments Using the CSP Approach

    DEFF Research Database (Denmark)

    Park, Youngsoo; Khosiawan, Yohanes; Moon, Ilkyeong

    2016-01-01

    In recent years there has been an increased demand in use of multiple unmanned aerial vehicles (UAVs) for surveillance and material handling tasks in indoor environments. However, only a limited number of studies have been reported on UAV scheduling in an indoor 3D environment. This paper present...

  9. Increasing the fidelity of noncanonical amino acid incorporation in cell-free protein synthesis.

    Science.gov (United States)

    Gan, Qinglei; Fan, Chenguang

    2017-11-01

    Cell-free protein synthesis provides a robust platform for co-translational incorporation of noncanonical amino acid (ncAA) into proteins to facilitate biological studies and biotechnological applications. Recently, eliminating the activity of release factor 1 has been shown to increase ncAA incorporation in response to amber codons. However, this approach could promote mis-incorporation of canonical amino acids by near cognate suppression. We performed a facile protocol to remove near cognate tRNA isoacceptors of the amber codon from total tRNAs, and used the phosphoserine (Sep) incorporation system as validation. By manipulating codon usage of target genes and tRNA species introduced into the cell-free protein synthesis system, we increased the fidelity of Sep incorporation at a specific position. By removing three near cognate tRNA isoacceptors of the amber stop codon [tRNA Lys , tRNA Tyr , and tRNA Gln (CUG)] from the total tRNA, the near cognate suppression decreased by 5-fold without impairing normal protein synthesis in the cell-free protein synthesis system. Mass spectrometry analyses indicated that the fidelity of ncAA incorporation was improved. Removal of near cognate tRNA isoacceptors of the amber codon could increase ncAA incorporation fidelity towards the amber stop codon in release factor deficiency systems. We provide a general strategy to improve fidelity of ncAA incorporation towards stop, quadruplet and sense codons in cell-free protein synthesis systems. This article is part of a Special Issue entitled "Biochemistry of Synthetic Biology - Recent Developments" Guest Editor: Dr. Ilka Heinemann and Dr. Patrick O'Donoghue. Copyright © 2016 Elsevier B.V. All rights reserved.

  10. Exactly energy conserving semi-implicit particle in cell formulation

    International Nuclear Information System (INIS)

    Lapenta, Giovanni

    2017-01-01

    We report a new particle in cell (PIC) method based on the semi-implicit approach. The novelty of the new method is that unlike any of its semi-implicit predecessors at the same time it retains the explicit computational cycle and conserves energy exactly. Recent research has presented fully implicit methods where energy conservation is obtained as part of a non-linear iteration procedure. The new method (referred to as Energy Conserving Semi-Implicit Method, ECSIM), instead, does not require any non-linear iteration and its computational cycle is similar to that of explicit PIC. The properties of the new method are: i) it conserves energy exactly to round-off for any time step or grid spacing; ii) it is unconditionally stable in time, freeing the user from the need to resolve the electron plasma frequency and allowing the user to select any desired time step; iii) it eliminates the constraint of the finite grid instability, allowing the user to select any desired resolution without being forced to resolve the Debye length; iv) the particle mover has a computational complexity identical to that of the explicit PIC, only the field solver has an increased computational cost. The new ECSIM is tested in a number of benchmarks where accuracy and computational performance are tested. - Highlights: • We present a new fully energy conserving semi-implicit particle in cell (PIC) method based on the implicit moment method (IMM). The new method is called Energy Conserving Implicit Moment Method (ECIMM). • The novelty of the new method is that unlike any of its predecessors at the same time it retains the explicit computational cycle and conserves energy exactly. • The new method is unconditionally stable in time, freeing the user from the need to resolve the electron plasma frequency. • The new method eliminates the constraint of the finite grid instability, allowing the user to select any desired resolution without being forced to resolve the Debye length. • These

  11. Exactly energy conserving semi-implicit particle in cell formulation

    Energy Technology Data Exchange (ETDEWEB)

    Lapenta, Giovanni, E-mail: giovanni.lapenta@kuleuven.be

    2017-04-01

    We report a new particle in cell (PIC) method based on the semi-implicit approach. The novelty of the new method is that unlike any of its semi-implicit predecessors at the same time it retains the explicit computational cycle and conserves energy exactly. Recent research has presented fully implicit methods where energy conservation is obtained as part of a non-linear iteration procedure. The new method (referred to as Energy Conserving Semi-Implicit Method, ECSIM), instead, does not require any non-linear iteration and its computational cycle is similar to that of explicit PIC. The properties of the new method are: i) it conserves energy exactly to round-off for any time step or grid spacing; ii) it is unconditionally stable in time, freeing the user from the need to resolve the electron plasma frequency and allowing the user to select any desired time step; iii) it eliminates the constraint of the finite grid instability, allowing the user to select any desired resolution without being forced to resolve the Debye length; iv) the particle mover has a computational complexity identical to that of the explicit PIC, only the field solver has an increased computational cost. The new ECSIM is tested in a number of benchmarks where accuracy and computational performance are tested. - Highlights: • We present a new fully energy conserving semi-implicit particle in cell (PIC) method based on the implicit moment method (IMM). The new method is called Energy Conserving Implicit Moment Method (ECIMM). • The novelty of the new method is that unlike any of its predecessors at the same time it retains the explicit computational cycle and conserves energy exactly. • The new method is unconditionally stable in time, freeing the user from the need to resolve the electron plasma frequency. • The new method eliminates the constraint of the finite grid instability, allowing the user to select any desired resolution without being forced to resolve the Debye length. • These

  12. Analysis of Noise Mechanisms in Cell-Size Control.

    Science.gov (United States)

    Modi, Saurabh; Vargas-Garcia, Cesar Augusto; Ghusinga, Khem Raj; Singh, Abhyudai

    2017-06-06

    At the single-cell level, noise arises from multiple sources, such as inherent stochasticity of biomolecular processes, random partitioning of resources at division, and fluctuations in cellular growth rates. How these diverse noise mechanisms combine to drive variations in cell size within an isoclonal population is not well understood. Here, we investigate the contributions of different noise sources in well-known paradigms of cell-size control, such as adder (division occurs after adding a fixed size from birth), sizer (division occurs after reaching a size threshold), and timer (division occurs after a fixed time from birth). Analysis reveals that variation in cell size is most sensitive to errors in partitioning of volume among daughter cells, and not surprisingly, this process is well regulated among microbes. Moreover, depending on the dominant noise mechanism, different size-control strategies (or a combination of them) provide efficient buffering of size variations. We further explore mixer models of size control, where a timer phase precedes/follows an adder, as has been proposed in Caulobacter crescentus. Although mixing a timer and an adder can sometimes attenuate size variations, it invariably leads to higher-order moments growing unboundedly over time. This results in a power-law distribution for the cell size, with an exponent that depends inversely on the noise in the timer phase. Consistent with theory, we find evidence of power-law statistics in the tail of C. crescentus cell-size distribution, although there is a discrepancy between the observed power-law exponent and that predicted from the noise parameters. The discrepancy, however, is removed after data reveal that the size added by individual newborns in the adder phase itself exhibits power-law statistics. Taken together, this study provides key insights into the role of noise mechanisms in size homeostasis, and suggests an inextricable link between timer-based models of size control and

  13. Inaccurate DNA synthesis in cell extracts of yeast producing active human DNA polymerase iota.

    Science.gov (United States)

    Makarova, Alena V; Grabow, Corinn; Gening, Leonid V; Tarantul, Vyacheslav Z; Tahirov, Tahir H; Bessho, Tadayoshi; Pavlov, Youri I

    2011-01-31

    Mammalian Pol ι has an unusual combination of properties: it is stimulated by Mn(2+) ions, can bypass some DNA lesions and misincorporates "G" opposite template "T" more frequently than incorporates the correct "A." We recently proposed a method of detection of Pol ι activity in animal cell extracts, based on primer extension opposite the template T with a high concentration of only two nucleotides, dGTP and dATP (incorporation of "G" versus "A" method of Gening, abbreviated as "misGvA"). We provide unambiguous proof of the "misGvA" approach concept and extend the applicability of the method for the studies of variants of Pol ι in the yeast model system with different cation cofactors. We produced human Pol ι in baker's yeast, which do not have a POLI ortholog. The "misGvA" activity is absent in cell extracts containing an empty vector, or producing catalytically dead Pol ι, or Pol ι lacking exon 2, but is robust in the strain producing wild-type Pol ι or its catalytic core, or protein with the active center L62I mutant. The signature pattern of primer extension products resulting from inaccurate DNA synthesis by extracts of cells producing either Pol ι or human Pol η is different. The DNA sequence of the template is critical for the detection of the infidelity of DNA synthesis attributed to DNA Pol ι. The primer/template and composition of the exogenous DNA precursor pool can be adapted to monitor replication fidelity in cell extracts expressing various error-prone Pols or mutator variants of accurate Pols. Finally, we demonstrate that the mutation rates in yeast strains producing human DNA Pols ι and η are not elevated over the control strain, despite highly inaccurate DNA synthesis by their extracts.

  14. Inaccurate DNA synthesis in cell extracts of yeast producing active human DNA polymerase iota.

    Directory of Open Access Journals (Sweden)

    Alena V Makarova

    2011-01-01

    Full Text Available Mammalian Pol ι has an unusual combination of properties: it is stimulated by Mn(2+ ions, can bypass some DNA lesions and misincorporates "G" opposite template "T" more frequently than incorporates the correct "A." We recently proposed a method of detection of Pol ι activity in animal cell extracts, based on primer extension opposite the template T with a high concentration of only two nucleotides, dGTP and dATP (incorporation of "G" versus "A" method of Gening, abbreviated as "misGvA". We provide unambiguous proof of the "misGvA" approach concept and extend the applicability of the method for the studies of variants of Pol ι in the yeast model system with different cation cofactors. We produced human Pol ι in baker's yeast, which do not have a POLI ortholog. The "misGvA" activity is absent in cell extracts containing an empty vector, or producing catalytically dead Pol ι, or Pol ι lacking exon 2, but is robust in the strain producing wild-type Pol ι or its catalytic core, or protein with the active center L62I mutant. The signature pattern of primer extension products resulting from inaccurate DNA synthesis by extracts of cells producing either Pol ι or human Pol η is different. The DNA sequence of the template is critical for the detection of the infidelity of DNA synthesis attributed to DNA Pol ι. The primer/template and composition of the exogenous DNA precursor pool can be adapted to monitor replication fidelity in cell extracts expressing various error-prone Pols or mutator variants of accurate Pols. Finally, we demonstrate that the mutation rates in yeast strains producing human DNA Pols ι and η are not elevated over the control strain, despite highly inaccurate DNA synthesis by their extracts.

  15. Abnormal expression of leiomyoma cytoskeletal proteins involved in cell migration.

    Science.gov (United States)

    Ura, Blendi; Scrimin, Federica; Arrigoni, Giorgio; Athanasakis, Emmanouil; Aloisio, Michelangelo; Monasta, Lorenzo; Ricci, Giuseppe

    2016-05-01

    Uterine leiomyomas are monoclonal tumors. Several factors are involved in the neoplastic transformation of the myometrium. In our study we focused on dysregulated cytoskeletal proteins in the leiomyoma as compared to the myometrium. Paired tissue samples of ten leiomyomas and adjacent myometria were obtained and analyzed by two‑dimensional gel electrophoresis (2-DE). Mass spectrometry was used for protein identification, and western blotting for 2-DE data validation. The values of ten cytoskeletal proteins were found to be significantly different: eight proteins were upregulated in the leiomyoma and two proteins were downregulated. Three of the upregulated proteins (myosin regulatory light polypeptide 9, four and a half LIM domains protein 1 and LIM and SH3 domain protein 1) are involved in cell migration, while downregulated protein transgelin is involved in replicative senescence. Myosin regulatory light polypeptide 9 (MYL9) was further validated by western blotting because it is considered to be a cell migration marker in several cancers and could play a key role in leiomyoma development. Our data demonstrate significant alterations in the expression of cytoskeletal proteins involved in leiomyoma growth. A better understanding of the involvement of cytoskeletal proteins in leiomyoma pathogenesis may contribute to the identification of new therapeutic targets and the development of new pharmacological approaches.

  16. Nanodiamond internalization in cells and the cell uptake mechanism

    Energy Technology Data Exchange (ETDEWEB)

    Perevedentseva, E. [National Dong Hwa University, Department of Physics (China); Hong, S.-F.; Huang, K.-J. [National Dong Hwa University, Department of Life Sciences (China); Chiang, I.-T.; Lee, C.-Y. [National Dong Hwa University, Department of Physics (China); Tseng, Y.-T. [National Dong Hwa University, Department of Life Sciences (China); Cheng, C.-L., E-mail: clcheng@mail.ndhu.edu.tw [National Dong Hwa University, Department of Physics (China)

    2013-08-15

    Cell type-dependent penetration of nanodiamond in living cells is one of the important factors for using nanodiamond as cellular markers/labels, for drug delivery as well as for other biomedical applications. In this work, internalization of 100 nm nanodiamonds by A549 lung human adenocarcinoma cell, Beas-2b non-tumorigenic human bronchial epithelial cell, and HFL-1 fibroblast-like human fetal lung cell is studied and compared. The penetration of nanodiamond into the cells was observed using confocal fluorescence imaging and Raman imaging methods. Visualization of the nanodiamond in cells allows comparison of the internalization for diamond nanoparticles in cancer A549 cell, non-cancer HFL-1, and Beas-2b cells. The dose-dependent and time-dependent behavior of nanodiamond uptake is observed in both cancer as well as non-cancer cells. The mechanism of nanodiamond uptake by cancer and non-cancer cells is analyzed by blocking different pathways. The uptake of nanodiamond in both cancer and non-cancer cells was found predominantly via clathrin-dependent endocytosis. In spite of observed similarity in the uptake mechanism for cancer and non-cancer cells, the nanodiamond uptake for cancer cell quantitatively exceeds the uptake for non-cancer cells, for the studied cell lines. The observed difference in internalization of nanodiamond by cancer and non-cancer cells is discussed.

  17. Detection of protease activity in cells and animals.

    Science.gov (United States)

    Verdoes, Martijn; Verhelst, Steven H L

    2016-01-01

    Proteases are involved in a wide variety of biologically and medically important events. They are entangled in a complex network of processes that regulate their activity, which makes their study intriguing, but challenging. For comprehensive understanding of protease biology and effective drug discovery, it is therefore essential to study proteases in models that are close to their complex native environments such as live cells or whole organisms. Protease activity can be detected by reporter substrates and activity-based probes, but not all of these reagents are suitable for intracellular or in vivo use. This review focuses on the detection of proteases in cells and in vivo. We summarize the use of probes and substrates as molecular tools, discuss strategies to deliver these tools inside cells, and describe sophisticated read-out techniques such as mass spectrometry and various imaging applications. This article is part of a Special Issue entitled: Physiological Enzymology and Protein Functions. Copyright © 2015 Elsevier B.V. All rights reserved.

  18. Particle in cell simulation of peaking switch for breakdown evaluation

    Energy Technology Data Exchange (ETDEWEB)

    Umbarkar, Sachin B.; Bindu, S.; Mangalvedekar, H.A.; Saxena, A.; Singh, N.M., E-mail: sachin.b.umbarkar@gmail.com [Department of Electric Engineering, Veermata Jijabai Technological Institute, Mumbai (India); Sharma, Archana; Saroj, P.C.; Mittal, K.C. [Accelerator Pulse Power Division, Bhabha Atomic Research Centre, Mumbai (India)

    2014-07-01

    Marx generator connected to peaking capacitor and peaking switch can generate Ultra-Wideband (UWB) radiation. A new peaking switch is designed for converting the existing nanosecond Marx generator to a UWB source. The paper explains the particle in cell (PIC) simulation for this peaking switch, using MAGIC 3D software. This peaking switch electrode is made up of copper tungsten material and is fixed inside the hermitically sealed derlin material. The switch can withstand a gas pressure up to 13.5 kg/cm{sup 2}. The lower electrode of the switch is connected to the last stage of the Marx generator. Initially Marx generator (without peaking stage) in air; gives the output pulse with peak amplitude of 113.75 kV and pulse rise time of 25 ns. Thus, we design a new peaking switch to improve the rise time of output pulse and to pressurize this peaking switch separately (i.e. Marx and peaking switch is at different pressure). The PIC simulation gives the particle charge density, current density, E counter plot, emitted electron current, and particle energy along the axis of gap between electrodes. The charge injection and electric field dependence on ionic dissociation phenomenon are briefly analyzed using this simulation. The model is simulated with different gases (N{sub 2}, H{sub 2}, and Air) under different pressure (2 kg/cm{sup 2}, 5 kg/cm{sup 2}, 10 kg/cm{sup 2}). (author)

  19. Particle-in-cell simulations of the lasertron

    International Nuclear Information System (INIS)

    Jones, M.E.; Peter, W.K.

    1985-01-01

    The lasertron is a device (either rf or dc) for producing intense, very short, pulsed electron beams (tens of picoseconds). In the dc lasertron, a laser is pulsed repetitively onto a photoemissive cathode. In general, the current is not space-charge limited and follows the laser intensity. The electron pulse is then accelerated out of the device by a constant voltage. By using the laser the need for a subharmonic buncher is eliminated. In the rf lasertron, the diode becomes an rf cavity. This improves the breakdown characteristics of the device, allowing higher voltages to be applied and hence higher currents to be obtained. The calculations are aimed at producing a 10 nC electron beam with an emittance of less than 40π mm-mrad for use in free-electron laser experiments at Los Alamos. Other applications of the lasertron include efficient microwave or rf generation. A class of electrode shapes has been obtained which in the absence of space charge produces no emittance growth. These shapes have been studied with the particle-in-cell simulation model ISIS, and the electrodes which produce minimum emittance including the effect of space charge have been determined. Unique emittance problems associated with the time dependence of the beam pulse are studied and conditions for reducing these effects are discussed. 5 refs., 5 figs

  20. Membrane tension and cytoskeleton organization in cell motility

    International Nuclear Information System (INIS)

    Sens, Pierre; Plastino, Julie

    2015-01-01

    Cell membrane shape changes are important for many aspects of normal biological function, such as tissue development, wound healing and cell division and motility. Various disease states are associated with deregulation of how cells move and change shape, including notably tumor initiation and cancer cell metastasis. Cell motility is powered, in large part, by the controlled assembly and disassembly of the actin cytoskeleton. Much of this dynamic happens in close proximity to the plasma membrane due to the fact that actin assembly factors are membrane-bound, and thus actin filaments are generally oriented such that their growth occurs against or near the membrane. For a long time, the membrane was viewed as a relatively passive scaffold for signaling. However, results from the last five years show that this is not the whole picture, and that the dynamics of the actin cytoskeleton are intimately linked to the mechanics of the cell membrane. In this review, we summarize recent findings concerning the role of plasma membrane mechanics in cell cytoskeleton dynamics and architecture, showing that the cell membrane is not just an envelope or a barrier for actin assembly, but is a master regulator controlling cytoskeleton dynamics and cell polarity. (topical review)

  1. Nanodiamond internalization in cells and the cell uptake mechanism

    International Nuclear Information System (INIS)

    Perevedentseva, E.; Hong, S.-F.; Huang, K.-J.; Chiang, I.-T.; Lee, C.-Y.; Tseng, Y.-T.; Cheng, C.-L.

    2013-01-01

    Cell type-dependent penetration of nanodiamond in living cells is one of the important factors for using nanodiamond as cellular markers/labels, for drug delivery as well as for other biomedical applications. In this work, internalization of 100 nm nanodiamonds by A549 lung human adenocarcinoma cell, Beas-2b non-tumorigenic human bronchial epithelial cell, and HFL-1 fibroblast-like human fetal lung cell is studied and compared. The penetration of nanodiamond into the cells was observed using confocal fluorescence imaging and Raman imaging methods. Visualization of the nanodiamond in cells allows comparison of the internalization for diamond nanoparticles in cancer A549 cell, non-cancer HFL-1, and Beas-2b cells. The dose-dependent and time-dependent behavior of nanodiamond uptake is observed in both cancer as well as non-cancer cells. The mechanism of nanodiamond uptake by cancer and non-cancer cells is analyzed by blocking different pathways. The uptake of nanodiamond in both cancer and non-cancer cells was found predominantly via clathrin-dependent endocytosis. In spite of observed similarity in the uptake mechanism for cancer and non-cancer cells, the nanodiamond uptake for cancer cell quantitatively exceeds the uptake for non-cancer cells, for the studied cell lines. The observed difference in internalization of nanodiamond by cancer and non-cancer cells is discussed

  2. Membrane tension and cytoskeleton organization in cell motility.

    Science.gov (United States)

    Sens, Pierre; Plastino, Julie

    2015-07-15

    Cell membrane shape changes are important for many aspects of normal biological function, such as tissue development, wound healing and cell division and motility. Various disease states are associated with deregulation of how cells move and change shape, including notably tumor initiation and cancer cell metastasis. Cell motility is powered, in large part, by the controlled assembly and disassembly of the actin cytoskeleton. Much of this dynamic happens in close proximity to the plasma membrane due to the fact that actin assembly factors are membrane-bound, and thus actin filaments are generally oriented such that their growth occurs against or near the membrane. For a long time, the membrane was viewed as a relatively passive scaffold for signaling. However, results from the last five years show that this is not the whole picture, and that the dynamics of the actin cytoskeleton are intimately linked to the mechanics of the cell membrane. In this review, we summarize recent findings concerning the role of plasma membrane mechanics in cell cytoskeleton dynamics and architecture, showing that the cell membrane is not just an envelope or a barrier for actin assembly, but is a master regulator controlling cytoskeleton dynamics and cell polarity.

  3. Variations in cell morphology in the canine cruciate ligament complex.

    Science.gov (United States)

    Smith, K D; Vaughan-Thomas, A; Spiller, D G; Clegg, P D; Innes, J F; Comerford, E J

    2012-08-01

    Cell morphology may reflect the mechanical environment of tissues and influence tissue physiology and response to injury. Normal cruciate ligaments (CLs) from disease-free stifle joints were harvested from dog breeds with a high (Labrador retriever) and low (Greyhound) risk of cranial cruciate ligament (CCL) rupture. Antibodies against the cytoskeletal components vimentin and alpha tubulin were used to analyse cell morphology; nuclei were stained with 4',6-diamidino-2-phenylindole, and images were collected using conventional and confocal microscopy. Both cranial and caudal CLs contained cells of heterogenous morphologies. Cells were arranged between collagen bundles and frequently had cytoplasmic processes. Some of these processes were long (type A cells), others were shorter, thicker and more branched (type B cells), and some had no processes (type C cells). Processes were frequently shown to contact other cells, extending longitudinally and transversely through the CLs. Cells with longer processes had fusiform nuclei, and those with no processes had rounded nuclei and were more frequent in the mid-substance of both CLs. Cells with long processes were more commonly noted in the CLs of the Greyhound. As contact between cells may facilitate direct communication, variances in cell morphology between breeds at a differing risk of CCL rupture may reflect differences in CL physiology. Copyright © 2012 Elsevier Ltd. All rights reserved.

  4. Numerical heating in Particle-In-Cell simulations with Monte Carlo binary collisions

    Science.gov (United States)

    Alves, E. Paulo; Mori, Warren; Fiuza, Frederico

    2017-10-01

    The binary Monte Carlo collision (BMCC) algorithm is a robust and popular method to include Coulomb collision effects in Particle-in-Cell (PIC) simulations of plasmas. While a number of works have focused on extending the validity of the model to different physical regimes of temperature and density, little attention has been given to the fundamental coupling between PIC and BMCC algorithms. Here, we show that the coupling between PIC and BMCC algorithms can give rise to (nonphysical) numerical heating of the system, that can be far greater than that observed when these algorithms operate independently. This deleterious numerical heating effect can significantly impact the evolution of the simulated system particularly for long simulation times. In this work, we describe the source of this numerical heating, and derive scaling laws for the numerical heating rates based on the numerical parameters of PIC-BMCC simulations. We compare our theoretical scalings with PIC-BMCC numerical experiments, and discuss strategies to minimize this parasitic effect. This work is supported by DOE FES under FWP 100237 and 100182.

  5. Performance of particle in cell methods on highly concurrent computational architectures

    International Nuclear Information System (INIS)

    Adams, M.F.; Ethier, S.; Wichmann, N.

    2009-01-01

    Particle in cell (PIC) methods are effective in computing Vlasov-Poisson system of equations used in simulations of magnetic fusion plasmas. PIC methods use grid based computations, for solving Poisson's equation or more generally Maxwell's equations, as well as Monte-Carlo type methods to sample the Vlasov equation. The presence of two types of discretizations, deterministic field solves and Monte-Carlo methods for the Vlasov equation, pose challenges in understanding and optimizing performance on today large scale computers which require high levels of concurrency. These challenges arises from the need to optimize two very different types of processes and the interactions between them. Modern cache based high-end computers have very deep memory hierarchies and high degrees of concurrency which must be utilized effectively to achieve good performance. The effective use of these machines requires maximizing concurrency by eliminating serial or redundant work and minimizing global communication. A related issue is minimizing the memory traffic between levels of the memory hierarchy because performance is often limited by the bandwidths and latencies of the memory system. This paper discusses some of the performance issues, particularly in regard to parallelism, of PIC methods. The gyrokinetic toroidal code (GTC) is used for these studies and a new radial grid decomposition is presented and evaluated. Scaling of the code is demonstrated on ITER sized plasmas with up to 16K Cray XT3/4 cores.

  6. Performance of particle in cell methods on highly concurrent computational architectures

    International Nuclear Information System (INIS)

    Adams, M F; Ethier, S; Wichmann, N

    2007-01-01

    Particle in cell (PIC) methods are effective in computing Vlasov-Poisson system of equations used in simulations of magnetic fusion plasmas. PIC methods use grid based computations, for solving Poisson's equation or more generally Maxwell's equations, as well as Monte-Carlo type methods to sample the Vlasov equation. The presence of two types of discretizations, deterministic field solves and Monte-Carlo methods for the Vlasov equation, pose challenges in understanding and optimizing performance on today large scale computers which require high levels of concurrency. These challenges arises from the need to optimize two very different types of processes and the interactions between them. Modern cache based high-end computers have very deep memory hierarchies and high degrees of concurrency which must be utilized effectively to achieve good performance. The effective use of these machines requires maximizing concurrency by eliminating serial or redundant work and minimizing global communication. A related issue is minimizing the memory traffic between levels of the memory hierarchy because performance is often limited by the bandwidths and latencies of the memory system. This paper discusses some of the performance issues, particularly in regard to parallelism, of PIC methods. The gyrokinetic toroidal code (GTC) is used for these studies and a new radial grid decomposition is presented and evaluated. Scaling of the code is demonstrated on ITER sized plasmas with up to 16K Cray XT3/4 cores

  7. Supply Management Analysis of the Chilean Navy Acquisition System

    Science.gov (United States)

    2014-12-01

    52 LIST OF REFERENCES Armada de Chile, N. (1986). Manual de Logistica de la Armada de Chile [Manual of logistics of the Chilean Navy]. Chile... transportation • Quality control • Demand and supply planning • Receiving, materials handling, and storage 11 • Material or inventory control...Order purchasing • Production planning, scheduling, and control • Warehousing and distribution • Shipping • Outbound transportation • Customer

  8. Polyamines and polyamine biosynthesis in cells exposed to hyperthermia

    Energy Technology Data Exchange (ETDEWEB)

    Gerner, E.W.; Stickney, D.G.; Herman, T.S.; Fuller, D.J.

    1983-02-01

    The issue of how polyamines act to sensitize cultured cells to the lethal effects of hyperthermia was investigated using Chinese hamster cells which were induced to express thermotolerance. Intracellular levels of these naturally occurring polycations were manipulated in certain situations by treating whole cells with methylglyoxal bis-(guanylhydrazone), an inhibitor of the S-adenosyl-L-methionine decarboxylases. Exogenous spermine as low as 100 ..mu..M in the culture media dramatically sensitized cells expressing thermotolerance to the lethal effects of subsequent 42/sup 0/C exposures. When thermotolerance was differentially induced in cultures exposed to 42.4/sup 0/C by varying the rate of heating from 37 to 42.4/sup 0/C, the most resistant cells and the highest levels of intracellular spermidine and spermine. This finding was explainable in part by the observation that the putrescine-dependent S-adenosyl-L-methionine decarboxylase activity was minimally affected in cells expressng the greatest degree of thermotolerance. When this enzyme activity was inhibited by drug, lowered intracellular polyamine levels did not correspond with subsequent survival responses to heat. Interestingly, cultures treated with methylglyoxal bis-(guanylhydrazone) 24 hr previous to heat exposure showed a reduced capacity to express rate of heating-induced thermotolerance. Together, these results demonstrate that the polyamines, especially spermidine and spermine, enhance hyperthermia-induced cell killing by some mechanism involving the plasma membrane. Further, our data suggest that methylglyoxal bis-(guanylhydrazone) can act to affect thermal responses by a mechanism(s) other than modification of intracellular polyamine levels.

  9. Evaluation of safety of Hammada salicornica in cell culture

    Directory of Open Access Journals (Sweden)

    F. Hosseini Hamedani

    2017-11-01

    Full Text Available Background and objectives: A pharmaceutical products that is planned to be used in clinic, should not only have beneficial effects but also be safe too. Preclinical studies in animals are costly and need considering ethical issues. Cell culture can be used before animal tests. Considering useful effects of these methods, we have evaluated safety of total methanol extract of Hammada salicornica and its aqueous and petroleum ether fractions in cell culture.Methods: Total methanol extract was prepared with the standard method of maceration. Different fractions were prepared by liquid-liquid fractionation and the extracts were then dried with rotary evaporator. After determination of bactericidal concentration of the extracts, 400 ug/mL, the cytotoxicity was tested at various concentrations regarding the minimum antibacterial concentration by MTT test. Hep-2c and VERO cell lines were used in MTT test. A range of concentrations (10-500 ug/mL of the extracts were prepared and were added to about 70% confluent 96 well plates. After exposure for 48 h, MTT solution was added to the wells, and 4 h later formazan crystals were solubilized and optical densities were read at 570 nm. Results: Cytotoxicity Index was calculated and significance test was performed using t-test comparing the Index of the test and control group at each concentration. No significant difference was observed. Conclusion: Various fractions of H. salicornica were not cytotoxic at concentrations above bactericidal concentrations (up to 500 ug/mL. The results need to be confirmed in animal studies before using in human subjects.

  10. Particle-in-cell Simulation of Dipolarization Front Associated Whistlers

    Science.gov (United States)

    Lin, D.; Scales, W.; Ganguli, G.; Crabtree, C. E.

    2017-12-01

    Dipolarization fronts (DFs) are dipolarized magnetic field embedded in the Earthward propagating bursty bulk flows (BBFs), which separates the hot, tenuous high-speed flow from the cold, dense, and slowly convecting surrounding plasma [Runov et al. 2011]. Broadband fluctuations have been observed at DFs including the electromagnetic whistler waves and electrostatic lower hybrid waves in the Very Low Frequency (VLF) range [e.g., Zhou et al. 2009, Deng et al. 2010]. There waves are suggested to be able heat electrons and play a critical role in the plasma sheet dynamics [Chaston et al., 2012, Angelopoulos et al., 2013]. However, their generation mechanism and role in the energy conversion are still under debate. The gradient scale of magnetic field, plasma density at DFs in the near-Earth magnetotail is comparable to or lower than the ion gyro radius [Runov et al., 2011, Fu et al., 2012, Breuillard et al., 2016]. Such strongly inhomogeneous configuration could be unstable to the electron-ion hybrid (EIH) instability, which arises from strongly sheared transverse flow and is in the VLF range [Ganguli et al. 1988, Ganguli et al. 2014]. The equilibrium of the EIH theory implies an anisotropy of electron temperature, which are likely to drive the whistler waves observed in DFs [Deng et al., 2010, Gary et al., 2011]. In order to better understand how the whistler waves are generated in DFs and whether the EIH theory is applicable, a fully electromagnetic particle-in-cell (EMPIC) model is used to simulate the EIH instability with similar equilibrium configurations in DF observations. The EMPIC model deals with three dimensions in the velocity space and two dimensions in the configuration space, which is quite ready to include the third configuration dimension. Simulation results will be shown in this presentation.

  11. Identification of transcript regulatory patterns in cell differentiation.

    Science.gov (United States)

    Gusnanto, Arief; Gosling, John Paul; Pope, Christopher

    2017-10-15

    Studying transcript regulatory patterns in cell differentiation is critical in understanding its complex nature of the formation and function of different cell types. This is done usually by measuring gene expression at different stages of the cell differentiation. However, if the gene expression data available are only from the mature cells, we have some challenges in identifying transcript regulatory patterns that govern the cell differentiation. We propose to exploit the information of the lineage of cell differentiation in terms of correlation structure between cell types. We assume that two different cell types that are close in the lineage will exhibit many common genes that are co-expressed relative to those that are far in the lineage. Current analysis methods tend to ignore this correlation by testing for differential expression assuming some sort of independence between cell types. We employ a Bayesian approach to estimate the posterior distribution of the mean of expression in each cell type, by taking into account the cell formation path in the lineage. This enables us to infer genes that are specific in each cell type, indicating the genes are involved in directing the cell differentiation to that particular cell type. We illustrate the method using gene expression data from a study of haematopoiesis. R codes to perform the analysis are available in http://www1.maths.leeds.ac.uk/∼arief/R/CellDiff/. a.gusnanto@leeds.ac.uk. Supplementary data are available at Bioinformatics online. © The Author (2017). Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com

  12. Novel method for detection of glycogen in cells.

    Science.gov (United States)

    Skurat, Alexander V; Segvich, Dyann M; DePaoli-Roach, Anna A; Roach, Peter J

    2017-05-01

    Glycogen, a branched polymer of glucose, functions as an energy reserve in many living organisms. Abnormalities in glycogen metabolism, usually excessive accumulation, can be caused genetically, most often through mutation of the enzymes directly involved in synthesis and degradation of the polymer leading to a variety of glycogen storage diseases (GSDs). Microscopic visualization of glycogen deposits in cells and tissues is important for the study of normal glycogen metabolism as well as diagnosis of GSDs. Here, we describe a method for the detection of glycogen using a renewable, recombinant protein which contains the carbohydrate-binding module (CBM) from starch-binding domain containing protein 1 (Stbd1). We generated a fusion protein containing g lutathione S-transferase, a cM c eptitope and the tbd1 BM (GYSC) for use as a glycogen-binding probe, which can be detected with secondary antibodies against glutathione S-transferase or cMyc. By enzyme-linked immunosorbent assay, we demonstrate that GYSC binds glycogen and two other polymers of glucose, amylopectin and amylose. Immunofluorescence staining of cultured cells indicate a GYSC-specific signal that is co-localized with signals obtained with anti-glycogen or anti-glycogen synthase antibodies. GYSC-positive staining inside of lysosomes is observed in individual muscle fibers isolated from mice deficient in lysosomal enzyme acid alpha-glucosidase, a well-characterized model of GSD II (Pompe disease). Co-localized GYSC and glycogen signals are also found in muscle fibers isolated from mice deficient in malin, a model for Lafora disease. These data indicate that GYSC is a novel probe that can be used to study glycogen metabolism under normal and pathological conditions. © The Author 2017. Published by Oxford University Press. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com

  13. Explicit tracking of uncertainty increases the power of quantitative rule-of-thumb reasoning in cell biology.

    Science.gov (United States)

    Johnston, Iain G; Rickett, Benjamin C; Jones, Nick S

    2014-12-02

    Back-of-the-envelope or rule-of-thumb calculations involving rough estimates of quantities play a central scientific role in developing intuition about the structure and behavior of physical systems, for example in so-called Fermi problems in the physical sciences. Such calculations can be used to powerfully and quantitatively reason about biological systems, particularly at the interface between physics and biology. However, substantial uncertainties are often associated with values in cell biology, and performing calculations without taking this uncertainty into account may limit the extent to which results can be interpreted for a given problem. We present a means to facilitate such calculations where uncertainties are explicitly tracked through the line of reasoning, and introduce a probabilistic calculator called CALADIS, a free web tool, designed to perform this tracking. This approach allows users to perform more statistically robust calculations in cell biology despite having uncertain values, and to identify which quantities need to be measured more precisely to make confident statements, facilitating efficient experimental design. We illustrate the use of our tool for tracking uncertainty in several example biological calculations, showing that the results yield powerful and interpretable statistics on the quantities of interest. We also demonstrate that the outcomes of calculations may differ from point estimates when uncertainty is accurately tracked. An integral link between CALADIS and the BioNumbers repository of biological quantities further facilitates the straightforward location, selection, and use of a wealth of experimental data in cell biological calculations. Copyright © 2014 The Authors. Published by Elsevier Inc. All rights reserved.

  14. Nonhomologous DNA End Joining in Cell-Free Extracts

    Directory of Open Access Journals (Sweden)

    Sheetal Sharma

    2010-01-01

    Full Text Available Among various DNA damages, double-strand breaks (DSBs are considered as most deleterious, as they may lead to chromosomal rearrangements and cancer when unrepaired. Nonhomologous DNA end joining (NHEJ is one of the major DSB repair pathways in higher organisms. A large number of studies on NHEJ are based on in vitro systems using cell-free extracts. In this paper, we summarize the studies on NHEJ performed by various groups in different cell-free repair systems.

  15. CXCR7 controls competition for recruitment of β-arrestin 2 in cells expressing both CXCR4 and CXCR7.

    Directory of Open Access Journals (Sweden)

    Nathaniel L Coggins

    Full Text Available Chemokine CXCL12 promotes growth and metastasis of more than 20 different human cancers, as well as pathogenesis of other common diseases. CXCL12 binds two different receptors, CXCR4 and CXCR7, both of which recruit and signal through the cytosolic adapter protein β-arrestin 2. Differences in CXCL12-dependent recruitment of β-arrestin 2 in cells expressing one or both receptors remain poorly defined. To quantitatively investigate parameters controlling association of β-arrestin 2 with CXCR4 or CXCR7 in cells co-expressing both receptors, we used a systems biology approach combining real-time, multi-spectral luciferase complementation imaging with computational modeling. Cells expressing only CXCR4 maintain low basal association with β-arrestin 2, and CXCL12 induces a rapid, transient increase in this interaction. In contrast, cells expressing only CXCR7 have higher basal association with β-arrestin 2 and exhibit more gradual, prolonged recruitment of β-arrestin 2 in response to CXCL12. We developed and fit a data-driven computational model for association of either CXCR4 or CXCR7 with β-arrestin 2 in cells expressing only one type of receptor. We then experimentally validated model predictions that co-expression of CXCR4 and CXCR7 on the same cell substantially decreases both the magnitude and duration of CXCL12-regulated recruitment of β-arrestin 2 to CXCR4. Co-expression of both receptors on the same cell only minimally alters recruitment of β-arrestin 2 to CXCR7. In silico experiments also identified β-arrestin 2 as a limiting factor in cells expressing both receptors, establishing that CXCR7 wins the "competition" with CXCR4 for CXCL12 and recruitment of β-arrestin 2. These results reveal how competition for β-arrestin 2 controls integrated responses to CXCL12 in cells expressing both CXCR4 and CXCR7. These results advance understanding of normal and pathologic functions of CXCL12, which is critical for developing effective

  16. LABILE IRON IN CELLS AND BODY FLUIDS . Physiology, Pathology and Pharmacology

    Directory of Open Access Journals (Sweden)

    Zvi Ioav Cabantchik

    2014-03-01

    Full Text Available In living systems iron appears predominantly associated with proteins, but can also be detected in forms referred as labile iron, which denotes the combined redox properties of iron and its amenability to exchange between ligands, including chelators. The labile cell iron (LCI composition varies with metal concentration and substances with chelating groups but also with pH and the redox potential. Although physiologically in the lower µM range, LCI plays a key role in cell iron economy as cross-roads of metabolic pathways. LCI levels are continually regulated by an iron-responsive machinery that balances iron uptake versus deposition into ferritin. However, LCI rises aberrantly in some cell types due to faulty cell utilization pathways or infiltration by pathological iron forms that are found in hemosiderotic plasma. As LCI attains pathological levels, it can catalyze reactive O species (ROS formation that, at particular threshold, can surpass cellular anti-oxidant capacities and seriously damage its constituents. While in normal plasma and interstitial fluids, virtually all iron is securely carried by circulating transferrin (that renders iron essentially non-labile, in systemic iron overload (IO, the total plasma iron binding capacity is often surpassed by a massive iron influx from hyperabsorptive gut or from erythrocyte overburdened spleen and/or liver. As plasma transferrin approaches iron saturation, labile plasma iron (LPI emerges in forms that can infiltrate cells by unregulated routes and raise LCI to toxic levels. Despite the limited knowledge available on LPI speciation in different types and degrees of iron overload, LPI measurements can be and are in fact used for identifying systemic IO and for initiating/adjusting chelation regimens to attain full-day LPI protection. A recent application of labile iron assay is the detection of labile components in iv iron formulations per se as well as in plasma (LPI following parenteral iron

  17. Protective layer formation on magnesium in cell culture medium

    Energy Technology Data Exchange (ETDEWEB)

    Wagener, V.; Virtanen, S., E-mail: virtanen@ww.uni-erlangen.de

    2016-06-01

    In the past, different studies showed that hydroxyapatite (HA) or similar calcium phosphates can be precipitated on Mg during immersion in simulated body fluids. However, at the same time, in most cases a dark grey or black layer is built under the white HA crystals. This layer seems to consist as well of calcium phosphates. Until now, neither the morphology nor its influence on Mg corrosion have been investigated in detail. In this work commercially pure magnesium (cp) was immersed in cell culture medium for one, three and five days at room temperature and in the incubator (37 °C, 5% CO{sub 2}). In addition, the influence of proteins on the formation of a corrosion layer was investigated by adding 20% of fetal calf serum (FCS) to the cell culture medium in the incubator. In order to analyze the formed layers, SEM images of cross sections, X-ray Photoelectron Spectroscopy (XPS), X-ray diffraction (XRD), Energy Dispersive X-ray Spectroscopy (EDX) and Fourier Transformed Infrared Spectroscopy (FTIR) measurements were carried out. Characterization of the corrosion behavior was achieved by electrochemical impedance spectroscopy (EIS) and by potentio-dynamic polarization in Dulbecco's Modified Eagle's Medium (DMEM) at 37 °C. Surface analysis showed that all formed layers consist mainly of amorphous calcium phosphate compounds. For the immersion at room temperature the Ca/P ratio indicates the formation of HA, while in the incubator probably pre-stages to HA are formed. The different immersion conditions lead to a variation in layer thicknesses. However, electrochemical characterization shows that the layer thickness does not influence the corrosion resistance of magnesium. The main influencing factor for the corrosion behavior is the layer morphology. Thus, immersion at room temperature leads to the highest corrosion protection due to the formation of a compact outer layer. Layers formed in the incubator show much worse performances due to completely porous

  18. Protective layer formation on magnesium in cell culture medium.

    Science.gov (United States)

    Wagener, V; Virtanen, S

    2016-06-01

    In the past, different studies showed that hydroxyapatite (HA) or similar calcium phosphates can be precipitated on Mg during immersion in simulated body fluids. However, at the same time, in most cases a dark grey or black layer is built under the white HA crystals. This layer seems to consist as well of calcium phosphates. Until now, neither the morphology nor its influence on Mg corrosion have been investigated in detail. In this work commercially pure magnesium (cp) was immersed in cell culture medium for one, three and five days at room temperature and in the incubator (37 °C, 5% CO2). In addition, the influence of proteins on the formation of a corrosion layer was investigated by adding 20% of fetal calf serum (FCS) to the cell culture medium in the incubator. In order to analyze the formed layers, SEM images of cross sections, X-ray Photoelectron Spectroscopy (XPS), X-ray diffraction (XRD), Energy Dispersive X-ray Spectroscopy (EDX) and Fourier Transformed Infrared Spectroscopy (FTIR) measurements were carried out. Characterization of the corrosion behavior was achieved by electrochemical impedance spectroscopy (EIS) and by potentio-dynamic polarization in Dulbecco's Modified Eagle's Medium (DMEM) at 37°C. Surface analysis showed that all formed layers consist mainly of amorphous calcium phosphate compounds. For the immersion at room temperature the Ca/P ratio indicates the formation of HA, while in the incubator probably pre-stages to HA are formed. The different immersion conditions lead to a variation in layer thicknesses. However, electrochemical characterization shows that the layer thickness does not influence the corrosion resistance of magnesium. The main influencing factor for the corrosion behavior is the layer morphology. Thus, immersion at room temperature leads to the highest corrosion protection due to the formation of a compact outer layer. Layers formed in the incubator show much worse performances due to completely porous structures. The

  19. Protective layer formation on magnesium in cell culture medium

    International Nuclear Information System (INIS)

    Wagener, V.; Virtanen, S.

    2016-01-01

    In the past, different studies showed that hydroxyapatite (HA) or similar calcium phosphates can be precipitated on Mg during immersion in simulated body fluids. However, at the same time, in most cases a dark grey or black layer is built under the white HA crystals. This layer seems to consist as well of calcium phosphates. Until now, neither the morphology nor its influence on Mg corrosion have been investigated in detail. In this work commercially pure magnesium (cp) was immersed in cell culture medium for one, three and five days at room temperature and in the incubator (37 °C, 5% CO_2). In addition, the influence of proteins on the formation of a corrosion layer was investigated by adding 20% of fetal calf serum (FCS) to the cell culture medium in the incubator. In order to analyze the formed layers, SEM images of cross sections, X-ray Photoelectron Spectroscopy (XPS), X-ray diffraction (XRD), Energy Dispersive X-ray Spectroscopy (EDX) and Fourier Transformed Infrared Spectroscopy (FTIR) measurements were carried out. Characterization of the corrosion behavior was achieved by electrochemical impedance spectroscopy (EIS) and by potentio-dynamic polarization in Dulbecco's Modified Eagle's Medium (DMEM) at 37 °C. Surface analysis showed that all formed layers consist mainly of amorphous calcium phosphate compounds. For the immersion at room temperature the Ca/P ratio indicates the formation of HA, while in the incubator probably pre-stages to HA are formed. The different immersion conditions lead to a variation in layer thicknesses. However, electrochemical characterization shows that the layer thickness does not influence the corrosion resistance of magnesium. The main influencing factor for the corrosion behavior is the layer morphology. Thus, immersion at room temperature leads to the highest corrosion protection due to the formation of a compact outer layer. Layers formed in the incubator show much worse performances due to completely porous structures. The

  20. Guide for training nuclear power plant operators

    International Nuclear Information System (INIS)

    Cox, J.A.; Cagle, C.D.; Corbett, B.L.; Culbert, W.H.; Hamrick, T.P.; Hurt, S.S.; McCord, R.V.; Poteet, K.H.; Bates, A.E.G.; Casto, W.R.

    1977-01-01

    Topics covered include basic preparation, radiation safety and control, principles of reactor operation, general operating characteristics, facility design, safety systems, instrumentation, reactor theory, fuel handling and core parameters, radioactive material handling, and administrative procedures

  1. Computer modeling describes gravity-related adaptation in cell cultures.

    Science.gov (United States)

    Alexandrov, Ludmil B; Alexandrova, Stoyana; Usheva, Anny

    2009-12-16

    Questions about the changes of biological systems in response to hostile environmental factors are important but not easy to answer. Often, the traditional description with differential equations is difficult due to the overwhelming complexity of the living systems. Another way to describe complex systems is by simulating them with phenomenological models such as the well-known evolutionary agent-based model (EABM). Here we developed an EABM to simulate cell colonies as a multi-agent system that adapts to hyper-gravity in starvation conditions. In the model, the cell's heritable characteristics are generated and transferred randomly to offspring cells. After a qualitative validation of the model at normal gravity, we simulate cellular growth in hyper-gravity conditions. The obtained data are consistent with previously confirmed theoretical and experimental findings for bacterial behavior in environmental changes, including the experimental data from the microgravity Atlantis and the Hypergravity 3000 experiments. Our results demonstrate that it is possible to utilize an EABM with realistic qualitative description to examine the effects of hypergravity and starvation on complex cellular entities.

  2. Historical Perspectives and Current Challenges in Cell Microencapsulation

    NARCIS (Netherlands)

    de Vos, Paul; Opara, Emmanuel C.

    2017-01-01

    The principle of immunoisolation of cells is based on encapsulation of cells in immunoprotective but semipermeable membranes that protect cells from hazardous effects of the host immune system but allows ingress of nutrients and outgress of therapeutic molecules. The technology was introduced in

  3. Graphene-augmented nanofiber scaffolds demonstrate new features in cells behaviour

    Science.gov (United States)

    Kazantseva, Jekaterina; Ivanov, Roman; Gasik, Michael; Neuman, Toomas; Hussainova, Irina

    2016-07-01

    Three-dimensional (3D) customized scaffolds capable to mimic a native extracellular matrix open new frontiers in cells manipulation and advanced therapy. The major challenge is in a proper substrate for in vitro models on engineered scaffolds, capable to modulate cells differentiation. Here for the first time we demonstrate novel design and functionality of the 3D porous scaffolds of aligned, self-assembled ceramic nanofibers of ultra-high anisotropy ratio (~107), augmented into graphene shells. This unique hybrid nano-network allows an exceptional combination of selective guidance stimuli of stem cells differentiation, immune reactions variations, and local immobilization of cancer cells, which was not available before. The scaffolds were shown to be able to direct human mesenchymal stem cells (important for stimulation of neuronal and muscle cells) preferential orientation, to suppress major inflammatory factors, and to localize cancer cells; all without additions of specific culture media. The selective downregulation of specific cytokines is anticipated as a new tool for understanding of human immune system and ways of treatment of associated diseases. The effects observed are self-regulated by cells only, without side effects, usually arising from use of external factors. New scaffolds may open new horizons for stem cells fate control such as towards axons and neurites regeneration (Alzheimer’s disease) as well as cancer therapy development.

  4. Electron behavior in ion beam neutralization in electric propulsion: full particle-in-cell simulation

    International Nuclear Information System (INIS)

    Usui, Hideyuki; Hashimoto, Akihiko; Miyake, Yohei

    2013-01-01

    By performing full Particle-In-Cell simulations, we examined the transient response of electrons released for the charge neutralization of a local ion beam emitted from an ion engine which is one of the electric propulsion systems. In the vicinity of the engine, the mixing process of electrons in the ion beam region is not so obvious because of large difference of dynamics between electrons and ions. A heavy ion beam emitted from a spacecraft propagates away from the engine and forms a positive potential region with respect to the background. Meanwhile electrons emitted for a neutralizer located near the ion engine are electrically attracted or accelerated to the core of the ion beam. Some electrons with the energy lower than the ion beam potential are trapped in the beam region and move along with the ion beam propagation with a multi-streaming structure in the beam potential region. Since the locations of the neutralizer and the ion beam exit are different, the above-mentioned bouncing motion of electrons is also observed in the direction of the beam diameter

  5. Readthrough of stop codons by use of aminoglycosides in cells from xeroderma pigmentosum group C patients.

    Science.gov (United States)

    Kuschal, Christiane; Khan, Sikandar G; Enk, Benedikt; DiGiovanna, John J; Kraemer, Kenneth H

    2015-04-01

    Readthrough of premature termination (stop) codons (PTC) is a new approach to treatment of genetic diseases. We recently reported that readthrough of PTC in cells from some xeroderma pigmentosum complementation group C (XP-C) patients could be achieved with the aminoglycosides geneticin or gentamicin. We found that the response depended on several factors including the PTC sequence, its location within the gene and the aminoglycoside used. Here, we extended these studies to investigate the effects of other aminoglycosides that are already on the market. We reasoned that topical treatment could deliver much higher concentrations of drug to the skin, the therapeutic target, and thus increase the therapeutic effect while reducing renal or ototoxicity in comparison with systemic treatment. Our prior clinical studies indicated that only a few percent of normal XPC expression was associated with mild clinical disease. We found minimal cell toxicity in the XP-C cells with several aminoglycosides. We found increased XPC mRNA expression in PTC-containing XP-C cells with G418, paromomycin, neomycin and kanamycin and increased XPC protein expression with G418. We conclude that in selected patients with XP, topical PTC therapy can be investigated as a method of personalized medicine to alleviate their cutaneous symptoms. Published 2015. This article is a U.S. Government work and is in the public domain in the USA.

  6. Fraction Reduction in Membrane Systems

    Directory of Open Access Journals (Sweden)

    Ping Guo

    2014-01-01

    Full Text Available Fraction reduction is a basic computation for rational numbers. P system is a new computing model, while the current methods for fraction reductions are not available in these systems. In this paper, we propose a method of fraction reduction and discuss how to carry it out in cell-like P systems with the membrane structure and the rules with priority designed. During the application of fraction reduction rules, synchronization is guaranteed by arranging some special objects in these rules. Our work contributes to performing the rational computation in P systems since the rational operands can be given in the form of fraction.

  7. Biosynthesis of proteins and radiation effects in cells

    International Nuclear Information System (INIS)

    Kolomiets, K.D.

    1982-01-01

    Critical analysis of nowadays literature and own experimental data on importance of biosynthesis of proteins, their modification and functional activity in forming radiation effects in irradiated cells is given. A special place in the development of radiation injury of cellular structures and in reduction processes is allocated to molecular recognition. The data on the role of protein synthesis and molecular recognition in the reduction of main biological cell chromatin system are presented. The dependence of postradiation changes in the cell on structural and functional chromatin state is considered

  8. Development of teleoperated cleanup system

    International Nuclear Information System (INIS)

    Kim, Ki Ho; Park, J. J.; Yang, M. S.; Kwon, H. J.

    2005-01-01

    This report describes the development of a teleoperated cleanup system for use in a highly radioactive environment of DFDF(DUPIC Fuel Demonstration Facility) at KAERI where direct human access to the in-cell is strictly limited. The teleoperated cleanup system was designed to remotely remove contaminants placed or fixed on the floor surface of the hot-cell by mopping them with wet cloth. This cleanup system consists of a mopping slave, a mopping master and a control console. The mopping slave located at the in-cell comprises a mopping tool with a mopping cloth and a mobile platform, which were constructed in modules to facilitate maintenance. The mopping master that is an input device to control the mopping slave has kinematic dissimilarity with the mopping slave. The control console provides a means of bilateral control flows and communications between the mopping master and the mopping slave. In operation, the human operator from the out-of-cell performs a series of decontamination tasks remotely by manipulating the mopping slave located in-cell via a mopping master, having a sense of real mopping. The environmental and mechanical design considerations, and control systems of the developed teleoperated cleanup system are also described

  9. Nitrosothiol signaling and protein nitrosation in cell death.

    Science.gov (United States)

    Iyer, Anand Krishnan V; Rojanasakul, Yon; Azad, Neelam

    2014-11-15

    Nitric oxide, a reactive free radical, is an important signaling molecule that can lead to a plethora of cellular effects affecting homeostasis. A well-established mechanism by which NO manifests its effect on cellular functions is the post-translational chemical modification of cysteine thiols in substrate proteins by a process known as S-nitrosation. Studies that investigate regulation of cellular functions through NO have increasingly established S-nitrosation as the primary modulatory mechanism in their respective systems. There has been a substantial increase in the number of reports citing various candidate proteins undergoing S-nitrosation, which affects cell-death and -survival pathways in a number of tissues including heart, lung, brain and blood. With an exponentially growing list of proteins being identified as substrates for S-nitrosation, it is important to assimilate this information in different cell/tissue systems in order to gain an overall view of protein regulation of both individual proteins and a class of protein substrates. This will allow for broad mapping of proteins as a function of S-nitrosation, and help delineate their global effects on pathophysiological responses including cell death and survival. This information will not only provide a much better understanding of overall functional relevance of NO in the context of various disease states, it will also facilitate the generation of novel therapeutics to combat specific diseases that are driven by NO-mediated S-nitrosation. Copyright © 2014 Elsevier Inc. All rights reserved.

  10. Low-noise Collision Operators for Particle-in-cell Simulations

    International Nuclear Information System (INIS)

    Lewandowski, J.L.V.

    2005-01-01

    A new method to implement low-noise collision operators in particle-in-cell simulations is presented. The method is based on the fact that relevant collision operators can be included naturally in the Lagrangian formulation that exemplifies the particle-in-cell simulation method. Numerical simulations show that the momentum and energy conservation properties of the simulated plasma associated with the low-noise collision operator are improved as compared with standard collision algorithms based on random numbers

  11. Electron microbeam specifications for use in cell irradiation experiments

    International Nuclear Information System (INIS)

    Kim, E.-H.; Choi, M.-C.; Lee, D.-H.; Chang, M.; Kang, C.-S.

    2003-01-01

    The microbeam irradiation system was devised originally to identify the hit and unhit cells by confining the beam within the target cell. The major achievement through the microbeam experiment studies has turned out to be the discovery of the 'bystander effect'. Microbeam experiments have been performed with alpha and proton beams in major and with soft x-rays in minor. The study with electron microbeam has been deferred mainly due to the difficulty in confining the electron tracks within a single target cell. In this paper, the electron microbeam irradiation system under development in Korea is introduced in terms of the beam specifications. The KIRAMS electron microbeam irradiation system consists of an electron gun, a vacuum chamber for beam collimation into 5 μm in diameter and a biology stage. The beam characteristics in terms of current and energy spectrum of the electrons entering a target cell and its neighbor cells were investigated by Monte Carlo simulation for the electron source energies of 25, 50, 75 and 100 keV. Energy depositions in the target cell and the neighbor cells were also calculated. The beam attenuation in current and energy occurs while electrons pass through the 2 μm-thick Mylar vacuum window, 100 μm-thick air gap and the 2 μm-thick Mylar bottom of cell dish. With 25 keV electron source, 80 % of decrease in current and 30 % of decrease in average energy were estimated before entering the target cell. With 75 keV electron source, on the other hand, 55 % of decrease in current and less than 1 % of decrease in average energy were estimated. Average dose per single collimated electron emission was 0.067 cGy to the target cell nucleus of 5 μm in diameter and 0.030 cGy to the cytoplasm of 2.5 μm in thickness with 25 keV electron source while they were 0.15 cGy and 0.019 cGy, respectively, with 75 keV electron source. The multiple scattering of electrons resulted in energy deposition in the neighbor cells as well. Dose to the first

  12. A deformable particle-in-cell method for advective transport in geodynamic modeling

    Science.gov (United States)

    Samuel, Henri

    2018-06-01

    This paper presents an improvement of the particle-in-cell method commonly used in geodynamic modeling for solving pure advection of sharply varying fields. Standard particle-in-cell approaches use particle kernels to transfer the information carried by the Lagrangian particles to/from the Eulerian grid. These kernels are generally one-dimensional and non-evolutive, which leads to the development of under- and over-sampling of the spatial domain by the particles. This reduces the accuracy of the solution, and may require the use of a prohibitive amount of particles in order to maintain the solution accuracy to an acceptable level. The new proposed approach relies on the use of deformable kernels that account for the strain history in the vicinity of particles. It results in a significant improvement of the spatial sampling by the particles, leading to a much higher accuracy of the numerical solution, for a reasonable computational extra cost. Various 2D tests were conducted to compare the performances of the deformable particle-in-cell method with the particle-in-cell approach. These consistently show that at comparable accuracy, the deformable particle-in-cell method was found to be four to six times more efficient than standard particle-in-cell approaches. The method could be adapted to 3D space and generalized to cases including motionless transport.

  13. Imaging in cell-based therapy for neurodegenerative diseases

    International Nuclear Information System (INIS)

    Kirik, Deniz; Breysse, Nathalie; Bjoerklund, Tomas; Besret, Laurent; Hantraye, Philippe

    2005-01-01

    Fetal cell transplantation for the treatment of Parkinson's and Huntington's diseases has been developed over the past two decades and is now in early clinical testing phase. Direct assessment of the graft's survival, integration into the host brain and impact on neuronal functions requires advanced in vivo neuroimaging techniques. Owing to its high sensitivity, positron emission tomography is today the most widely used tool to evaluate the viability and function of the transplanted tissue in the brain. Nuclear magnetic resonance techniques are opening new possibilities for imaging neurochemical events in the brain. The ultimate goal will be to use the combination of multiple imaging modalities for complete functional monitoring of the repair processes in the central nervous system. (orig.)

  14. Changes in cell-mediated immunity in patients undergoing radiotherapy

    International Nuclear Information System (INIS)

    Rafla, S.; Yang, S.J.; Meleka, F.

    1978-01-01

    The cell-mediated immune status of 147 patients who received radiotherapy was evaluated using in vitro tests (PHA, E-rosette, and spontaneous blastogenesis) both before and 6 weeks after the end of radiation. All patients have verified malignancies, involving the bronchus in 29 cases, breast in 28, female genital system in 26, head and neck in 20 and bladder in 15. Patients suffering from bronchogenic carcinomas or malignancies of the head and neck showed a relative high degree of immune suppression. Our findings indicate a trend towards some improvement in PHA reactivity, as well as in the percentage of E-rosette-forming cells after treatment, which is more noticeable in patients with pelvic or breast tumors. A relationship seems to exist between the tumor load and the immune status, which reverts to a normal pattern when the former is extinguished. Moreover, patients with poor clinical response display a profoundly depressed level of immune status without any improvement after treatment

  15. Synthetic biology in cell-based cancer immunotherapy.

    Science.gov (United States)

    Chakravarti, Deboki; Wong, Wilson W

    2015-08-01

    The adoptive transfer of genetically engineered T cells with cancer-targeting receptors has shown tremendous promise for eradicating tumors in clinical trials. This form of cellular immunotherapy presents a unique opportunity to incorporate advanced systems and synthetic biology approaches to create cancer therapeutics with novel functions. We first review the development of synthetic receptors, switches, and circuits to control the location, duration, and strength of T cell activity against tumors. In addition, we discuss the cellular engineering and genome editing of host cells (or the chassis) to improve the efficacy of cell-based cancer therapeutics, and to reduce the time and cost of manufacturing. Copyright © 2015 Elsevier Ltd. All rights reserved.

  16. Finding undetected protein associations in cell signaling by belief propagation.

    Science.gov (United States)

    Bailly-Bechet, M; Borgs, C; Braunstein, A; Chayes, J; Dagkessamanskaia, A; François, J-M; Zecchina, R

    2011-01-11

    External information propagates in the cell mainly through signaling cascades and transcriptional activation, allowing it to react to a wide spectrum of environmental changes. High-throughput experiments identify numerous molecular components of such cascades that may, however, interact through unknown partners. Some of them may be detected using data coming from the integration of a protein-protein interaction network and mRNA expression profiles. This inference problem can be mapped onto the problem of finding appropriate optimal connected subgraphs of a network defined by these datasets. The optimization procedure turns out to be computationally intractable in general. Here we present a new distributed algorithm for this task, inspired from statistical physics, and apply this scheme to alpha factor and drug perturbations data in yeast. We identify the role of the COS8 protein, a member of a gene family of previously unknown function, and validate the results by genetic experiments. The algorithm we present is specially suited for very large datasets, can run in parallel, and can be adapted to other problems in systems biology. On renowned benchmarks it outperforms other algorithms in the field.

  17. Hierarchical feedback modules and reaction hubs in cell signaling networks.

    Science.gov (United States)

    Xu, Jianfeng; Lan, Yueheng

    2015-01-01

    Despite much effort, identification of modular structures and study of their organizing and functional roles remain a formidable challenge in molecular systems biology, which, however, is essential in reaching a systematic understanding of large-scale cell regulation networks and hence gaining capacity of exerting effective interference to cell activity. Combining graph theoretic methods with available dynamics information, we successfully retrieved multiple feedback modules of three important signaling networks. These feedbacks are structurally arranged in a hierarchical way and dynamically produce layered temporal profiles of output signals. We found that global and local feedbacks act in very different ways and on distinct features of the information flow conveyed by signal transduction but work highly coordinately to implement specific biological functions. The redundancy embodied with multiple signal-relaying channels and feedback controls bestow great robustness and the reaction hubs seated at junctions of different paths announce their paramount importance through exquisite parameter management. The current investigation reveals intriguing general features of the organization of cell signaling networks and their relevance to biological function, which may find interesting applications in analysis, design and control of bio-networks.

  18. Hierarchical feedback modules and reaction hubs in cell signaling networks.

    Directory of Open Access Journals (Sweden)

    Jianfeng Xu

    Full Text Available Despite much effort, identification of modular structures and study of their organizing and functional roles remain a formidable challenge in molecular systems biology, which, however, is essential in reaching a systematic understanding of large-scale cell regulation networks and hence gaining capacity of exerting effective interference to cell activity. Combining graph theoretic methods with available dynamics information, we successfully retrieved multiple feedback modules of three important signaling networks. These feedbacks are structurally arranged in a hierarchical way and dynamically produce layered temporal profiles of output signals. We found that global and local feedbacks act in very different ways and on distinct features of the information flow conveyed by signal transduction but work highly coordinately to implement specific biological functions. The redundancy embodied with multiple signal-relaying channels and feedback controls bestow great robustness and the reaction hubs seated at junctions of different paths announce their paramount importance through exquisite parameter management. The current investigation reveals intriguing general features of the organization of cell signaling networks and their relevance to biological function, which may find interesting applications in analysis, design and control of bio-networks.

  19. Hierarchical Feedback Modules and Reaction Hubs in Cell Signaling Networks

    Science.gov (United States)

    Xu, Jianfeng; Lan, Yueheng

    2015-01-01

    Despite much effort, identification of modular structures and study of their organizing and functional roles remain a formidable challenge in molecular systems biology, which, however, is essential in reaching a systematic understanding of large-scale cell regulation networks and hence gaining capacity of exerting effective interference to cell activity. Combining graph theoretic methods with available dynamics information, we successfully retrieved multiple feedback modules of three important signaling networks. These feedbacks are structurally arranged in a hierarchical way and dynamically produce layered temporal profiles of output signals. We found that global and local feedbacks act in very different ways and on distinct features of the information flow conveyed by signal transduction but work highly coordinately to implement specific biological functions. The redundancy embodied with multiple signal-relaying channels and feedback controls bestow great robustness and the reaction hubs seated at junctions of different paths announce their paramount importance through exquisite parameter management. The current investigation reveals intriguing general features of the organization of cell signaling networks and their relevance to biological function, which may find interesting applications in analysis, design and control of bio-networks. PMID:25951347

  20. BeO-OSL detectors for dose measurements in cell cultures

    International Nuclear Information System (INIS)

    Andreeff, M.; Freudenberg, R.; Kotzerke, J.; Sommer, D.; Reichelt, U.; Henniger, J.

    2009-01-01

    Aim: The absorbed dose is an important parameter in experiments involving irradiation of cells in vitro with unsealed radionuclides. Typically, this is estimated with a model calculation, although the results thus obtained cannot be verified. Generally used real-time measurement methods are not applicable in this setting. A new detector material with in vitro suitability is the subject of this work. Methods: Optically-stimulated luminescence (OSL) dosimeters based on beryllium oxide (BeO) were used for dose measurement in cell cultures exposed to unsealed radionuclides. Their qualitative properties (e. g. energy-dependent count rate sensitivity, fading, contamination by radioactive liquids) were determined and compared to the results of a Monte Carlo simulation (using AMOS software). OSL dosimeters were tested in common cell culture setups with a known geometry. Results: Dose reproducibility of the OSL dosimeters was ± 1.5%. Fading at room temperature was 0.07% per day. Dose loss (optically-stimulated deletion) under ambient lighting conditions was 0.5% per minute. The Monte Carlo simulation for the relative sensitivity at different beta energies provided corresponding results to those obtained with the OSL dosimeters. Dose profile measurements using a 6 well plate and 14 ml PP tube showed that the geometry of the cell culture vessel has a marked influence on dose distribution with 188 Re. Conclusion: A new dosimeter system was calibrated with β-emitters of different energy. It turned out as suitable for measuring dose in liquids. The dose profile measurements obtained are suitably precise to be used as a check against theoretical dose calculations. (orig.)

  1. On the numerical dispersion of electromagnetic particle-in-cell code: Finite grid instability

    International Nuclear Information System (INIS)

    Meyers, M.D.; Huang, C.-K.; Zeng, Y.; Yi, S.A.; Albright, B.J.

    2015-01-01

    The Particle-In-Cell (PIC) method is widely used in relativistic particle beam and laser plasma modeling. However, the PIC method exhibits numerical instabilities that can render unphysical simulation results or even destroy the simulation. For electromagnetic relativistic beam and plasma modeling, the most relevant numerical instabilities are the finite grid instability and the numerical Cherenkov instability. We review the numerical dispersion relation of the Electromagnetic PIC model. We rigorously derive the faithful 3-D numerical dispersion relation of the PIC model, for a simple, direct current deposition scheme, which does not conserve electric charge exactly. We then specialize to the Yee FDTD scheme. In particular, we clarify the presence of alias modes in an eigenmode analysis of the PIC model, which combines both discrete and continuous variables. The manner in which the PIC model updates and samples the fields and distribution function, together with the temporal and spatial phase factors from solving Maxwell's equations on the Yee grid with the leapfrog scheme, is explicitly accounted for. Numerical solutions to the electrostatic-like modes in the 1-D dispersion relation for a cold drifting plasma are obtained for parameters of interest. In the succeeding analysis, we investigate how the finite grid instability arises from the interaction of the numerical modes admitted in the system and their aliases. The most significant interaction is due critically to the correct representation of the operators in the dispersion relation. We obtain a simple analytic expression for the peak growth rate due to this interaction, which is then verified by simulation. We demonstrate that our analysis is readily extendable to charge conserving models

  2. On the numerical dispersion of electromagnetic particle-in-cell code: Finite grid instability

    Science.gov (United States)

    Meyers, M. D.; Huang, C.-K.; Zeng, Y.; Yi, S. A.; Albright, B. J.

    2015-09-01

    The Particle-In-Cell (PIC) method is widely used in relativistic particle beam and laser plasma modeling. However, the PIC method exhibits numerical instabilities that can render unphysical simulation results or even destroy the simulation. For electromagnetic relativistic beam and plasma modeling, the most relevant numerical instabilities are the finite grid instability and the numerical Cherenkov instability. We review the numerical dispersion relation of the Electromagnetic PIC model. We rigorously derive the faithful 3-D numerical dispersion relation of the PIC model, for a simple, direct current deposition scheme, which does not conserve electric charge exactly. We then specialize to the Yee FDTD scheme. In particular, we clarify the presence of alias modes in an eigenmode analysis of the PIC model, which combines both discrete and continuous variables. The manner in which the PIC model updates and samples the fields and distribution function, together with the temporal and spatial phase factors from solving Maxwell's equations on the Yee grid with the leapfrog scheme, is explicitly accounted for. Numerical solutions to the electrostatic-like modes in the 1-D dispersion relation for a cold drifting plasma are obtained for parameters of interest. In the succeeding analysis, we investigate how the finite grid instability arises from the interaction of the numerical modes admitted in the system and their aliases. The most significant interaction is due critically to the correct representation of the operators in the dispersion relation. We obtain a simple analytic expression for the peak growth rate due to this interaction, which is then verified by simulation. We demonstrate that our analysis is readily extendable to charge conserving models.

  3. Melanopsin resets circadian rhythms in cells by inducing clock gene Period1

    Science.gov (United States)

    Yamashita, Shuhei; Uehara, Tomoe; Matsuo, Minako; Kikuchi, Yo; Numano, Rika

    2014-02-01

    The biochemical, physiological and behavioral processes are under the control of internal clocks with the period of approximately 24 hr, circadian rhythms. The expression of clock gene Period1 (Per1) oscillates autonomously in cells and is induced immediately after a light pulse. Per1 is an indispensable member of the central clock system to maintain the autonomous oscillator and synchronize environmental light cycle. Per1 expression could be detected by Per1∷luc and Per1∷GFP plasmid DNA in which firefly luciferase and Green Fluorescence Protein were rhythmically expressed under the control of the mouse Per1 promoter in order to monitor mammalian circadian rhythms. Membrane protein, MELANOPSIN is activated by blue light in the morning on the retina and lead to signals transduction to induce Per1 expression and to reset the phase of circadian rhythms. In this report Per1 induction was measured by reporter signal assay in Per1∷luc and Per1∷GFP fibroblast cell at the input process of circadian rhythms. To the result all process to reset the rhythms by Melanopsin is completed in single cell like in the retina projected to the central clock in the brain. Moreover, the phase of circadian rhythm in Per1∷luc cells is synchronized by photo-activated Melanopsin, because the definite peak of luciferase activity in one dish was found one day after light illumination. That is an available means that physiological circadian rhythms could be real-time monitor as calculable reporter (bioluminescent and fluorescent) chronological signal in both single and groups of cells.

  4. Prion Propagation in Cells Expressing PrP Glycosylation Mutants ▿

    Science.gov (United States)

    Salamat, Muhammad K.; Dron, Michel; Chapuis, Jérôme; Langevin, Christelle; Laude, Hubert

    2011-01-01

    Infection by prions involves conversion of a host-encoded cell surface protein (PrPC) to a disease-related isoform (PrPSc). PrPC carries two glycosylation sites variably occupied by complex N-glycans, which have been suggested by previous studies to influence the susceptibility to these diseases and to determine characteristics of prion strains. We used the Rov cell system, which is susceptible to sheep prions, to generate a series of PrPC glycosylation mutants with mutations at one or both attachment sites. We examined their subcellular trafficking and ability to convert into PrPSc and to sustain stable prion propagation in the absence of wild-type PrP. The susceptibility to infection of mutants monoglycosylated at either site differed dramatically depending on the amino acid substitution. Aglycosylated double mutants showed overaccumulation in the Golgi compartment and failed to be infected. Introduction of an ectopic glycosylation site near the N terminus fully restored cell surface expression of PrP but not convertibility into PrPSc, while PrPC with three glycosylation sites conferred cell permissiveness to infection similarly to the wild type. In contrast, predominantly aglycosylated molecules with nonmutated N-glycosylation sequons, produced in cells expressing glycosylphosphatidylinositol-anchorless PrPC, were able to form infectious PrPSc. Together our findings suggest that glycosylation is important for efficient trafficking of anchored PrP to the cell surface and sustained prion propagation. However, properly trafficked glycosylation mutants were not necessarily prone to conversion, thus making it difficult in such studies to discern whether the amino acid changes or glycan chain removal most influences the permissiveness to prion infection. PMID:21248032

  5. The thermodynamic efficiency of computations made in cells across the range of life

    Science.gov (United States)

    Kempes, Christopher P.; Wolpert, David; Cohen, Zachary; Pérez-Mercader, Juan

    2017-11-01

    Biological organisms must perform computation as they grow, reproduce and evolve. Moreover, ever since Landauer's bound was proposed, it has been known that all computation has some thermodynamic cost-and that the same computation can be achieved with greater or smaller thermodynamic cost depending on how it is implemented. Accordingly an important issue concerning the evolution of life is assessing the thermodynamic efficiency of the computations performed by organisms. This issue is interesting both from the perspective of how close life has come to maximally efficient computation (presumably under the pressure of natural selection), and from the practical perspective of what efficiencies we might hope that engineered biological computers might achieve, especially in comparison with current computational systems. Here we show that the computational efficiency of translation, defined as free energy expended per amino acid operation, outperforms the best supercomputers by several orders of magnitude, and is only about an order of magnitude worse than the Landauer bound. However, this efficiency depends strongly on the size and architecture of the cell in question. In particular, we show that the useful efficiency of an amino acid operation, defined as the bulk energy per amino acid polymerization, decreases for increasing bacterial size and converges to the polymerization cost of the ribosome. This cost of the largest bacteria does not change in cells as we progress through the major evolutionary shifts to both single- and multicellular eukaryotes. However, the rates of total computation per unit mass are non-monotonic in bacteria with increasing cell size, and also change across different biological architectures, including the shift from unicellular to multicellular eukaryotes. This article is part of the themed issue 'Reconceptualizing the origins of life'.

  6. Kinase Screening in Pichia pastoris Identified Promising Targets Involved in Cell Growth and Alcohol Oxidase 1 Promoter (PAOX1 Regulation.

    Directory of Open Access Journals (Sweden)

    Wei Shen

    Full Text Available As one of the most commonly used eukaryotic recombinant protein expression systems, P. pastoris relies heavily on the AOX1 promoter (PAOX1, which is strongly induced by methanol but strictly repressed by glycerol and glucose. However, the complicated signaling pathways involved in PAOX1 regulation when supplemented with different carbon sources are poorly understood. Here we constructed a kinase deletion library in P. pastoris and identified 27 mutants which showed peculiar phenotypes in cell growth or PAOX1 regulation. We analyzed both annotations and possible functions of these 27 targets, and then focused on the MAP kinase Hog1. In order to locate its potential downstream components, we performed the phosphoproteome analysis on glycerol cultured WT and Δhog1 strains and identified 157 differentially phosphorylated proteins. Our results identified important kinases involved in P. pastoris cell growth and PAOX1 regulation, which could serve as valuable targets for further mechanistic studies.

  7. Two-dimensional particle-in-cell simulation of the expansion of a plasma into a rarefied medium

    International Nuclear Information System (INIS)

    Sarri, G; Quinn, K; Kourakis, I; Borghesi, M; Murphy, G C; Drury, L O C; Dieckmann, M E; Ynnerman, A; Bret, A

    2011-01-01

    The expansion of a dense plasma through a more rarefied ionized medium has been studied by means of two-dimensional particle-in-cell simulations. The initial conditions involve a density jump by a factor of 100, located in the middle of an otherwise equally dense electron-proton plasma with uniform proton and electron temperatures of 10 eV and 1 keV, respectively. Simulations show the creation of a purely electrostatic collisionless shock together with an ion-acoustic soliton tied to its downstream region. The shock front is seen to evolve in filamentary structures consistently with the onset of the ion-ion instability. Meanwhile, an un-magnetized drift instability is triggered in the core part of the dense plasma. Such results explain recent experimental laser-plasma experiments, carried out in similar conditions, and are of intrinsic relevance to non-relativistic shock scenarios in the solar and astrophysical systems.

  8. Two-dimensional particle-in-cell simulation of the expansion of a plasma into a rarefied medium

    Energy Technology Data Exchange (ETDEWEB)

    Sarri, G; Quinn, K; Kourakis, I; Borghesi, M [Centre for Plasma Physics, The Queens University of Belfast, Belfast BT7 1NN (United Kingdom); Murphy, G C; Drury, L O C [Dublin Institute for Advanced Studies, 31 Fitzwilliam Place, Dublin 2 (Ireland); Dieckmann, M E; Ynnerman, A [Department of Science and Technology (ITN), Linkoeping University, 60174 Norrkoping (Sweden); Bret, A, E-mail: gsarri01@qub.ac.uk [ETSI Industriales, Universidad de Castilla-La Mancha, 13071 Ciudad Real (Spain)

    2011-07-15

    The expansion of a dense plasma through a more rarefied ionized medium has been studied by means of two-dimensional particle-in-cell simulations. The initial conditions involve a density jump by a factor of 100, located in the middle of an otherwise equally dense electron-proton plasma with uniform proton and electron temperatures of 10 eV and 1 keV, respectively. Simulations show the creation of a purely electrostatic collisionless shock together with an ion-acoustic soliton tied to its downstream region. The shock front is seen to evolve in filamentary structures consistently with the onset of the ion-ion instability. Meanwhile, an un-magnetized drift instability is triggered in the core part of the dense plasma. Such results explain recent experimental laser-plasma experiments, carried out in similar conditions, and are of intrinsic relevance to non-relativistic shock scenarios in the solar and astrophysical systems.

  9. Environmental tests of metallization systems for terrestrial photovoltaic cells

    Science.gov (United States)

    Alexander, P., Jr.

    1985-01-01

    Seven different solar cell metallization systems were subjected to temperature cycling tests and humidity tests. Temperature cycling excursions were -50 deg C to 150 deg C per cycle. Humidity conditions were 70 deg C at 98% relative humidity. The seven metallization systems were: Ti/Ag, Ti/Pd/Ag, Ti/Pd/Cu, Ni/Cu, Pd/Ni/Solder, Cr/Pd/Ag, and thick film Ag. All metallization systems showed a slight to moderate decrease in cell efficiencies after subjection to 1000 temperature cycles. Six of the seven metallization systems also evidenced slight increases in cell efficiencies after moderate numbers of cycles, generally less than 100 cycles. The copper based systems showed the largest decrease in cell efficiencies after temperature cycling. All metallization systems showed moderate to large decreases in cell efficiencies after 123 days of humidity exposure. The copper based systems again showed the largest decrease in cell efficiencies after humidity exposure. Graphs of the environmental exposures versus cell efficiencies are presented for each metallization system, as well as environmental exposures versus fill factors or series resistance.

  10. Silver nanoparticle protein corona composition in cell culture media.

    Science.gov (United States)

    Shannahan, Jonathan H; Lai, Xianyin; Ke, Pu Chun; Podila, Ramakrishna; Brown, Jared M; Witzmann, Frank A

    2013-01-01

    The potential applications of nanomaterials as drug delivery systems and in other products continue to expand. Upon introduction into physiological environments and driven by energetics, nanomaterials readily associate proteins forming a protein corona (PC) on their surface. This PC influences the nanomaterial's surface characteristics and may impact their interaction with cells. To determine the biological impact of nanomaterial exposure as well as nanotherapeutic applications, it is necessary to understand PC formation. Utilizing a label-free mass spectrometry-based proteomics approach, we examined the composition of the PC for a set of four silver nanoparticles (AgNPs) including citrate-stabilized and polyvinlypyrrolidone-stabilized (PVP) colloidal silver (20 or 110 nm diameter). To simulate cell culture conditions, AgNPs were incubated for 1 h in Dulbecco's Modified Eagle Medium supplemented with 10% fetal bovine serum, washed, coronal proteins solubilized, and proteins identified and quantified by label-free LC-MS/MS. To determine which attributes influence PC formation, the AgNPs were characterized in both water and cell culture media with 10% FBS. All AgNPs associated a common subset of 11 proteins including albumin, apolipoproteins, keratins, and other serum proteins. 110 nm citrate- and PVP-stabilized AgNPs were found to bind the greatest number of proteins (79 and 85 respectively) compared to 20 nm citrate- and PVP-stabilized AgNPs (45 and 48 respectively), suggesting a difference in PC formation based on surface curvature. While no relationships were found for other protein parameters (isoelectric point or aliphatic index), the PC on 20 nm AgNPs (PVP and citrate) consisted of more hydrophobic proteins compared to 110 nm AgNPs implying that this class of proteins are more receptive to curvature-induced folding and crowding in exchange for an increased hydration in the aqueous environment. These observations demonstrate the significance of electrostatic

  11. Silver nanoparticle protein corona composition in cell culture media.

    Directory of Open Access Journals (Sweden)

    Jonathan H Shannahan

    Full Text Available The potential applications of nanomaterials as drug delivery systems and in other products continue to expand. Upon introduction into physiological environments and driven by energetics, nanomaterials readily associate proteins forming a protein corona (PC on their surface. This PC influences the nanomaterial's surface characteristics and may impact their interaction with cells. To determine the biological impact of nanomaterial exposure as well as nanotherapeutic applications, it is necessary to understand PC formation. Utilizing a label-free mass spectrometry-based proteomics approach, we examined the composition of the PC for a set of four silver nanoparticles (AgNPs including citrate-stabilized and polyvinlypyrrolidone-stabilized (PVP colloidal silver (20 or 110 nm diameter. To simulate cell culture conditions, AgNPs were incubated for 1 h in Dulbecco's Modified Eagle Medium supplemented with 10% fetal bovine serum, washed, coronal proteins solubilized, and proteins identified and quantified by label-free LC-MS/MS. To determine which attributes influence PC formation, the AgNPs were characterized in both water and cell culture media with 10% FBS. All AgNPs associated a common subset of 11 proteins including albumin, apolipoproteins, keratins, and other serum proteins. 110 nm citrate- and PVP-stabilized AgNPs were found to bind the greatest number of proteins (79 and 85 respectively compared to 20 nm citrate- and PVP-stabilized AgNPs (45 and 48 respectively, suggesting a difference in PC formation based on surface curvature. While no relationships were found for other protein parameters (isoelectric point or aliphatic index, the PC on 20 nm AgNPs (PVP and citrate consisted of more hydrophobic proteins compared to 110 nm AgNPs implying that this class of proteins are more receptive to curvature-induced folding and crowding in exchange for an increased hydration in the aqueous environment. These observations demonstrate the significance of

  12. Multiplexed quantitative high content screening reveals that cigarette smoke condensate induces changes in cell structure and function through alterations in cell signaling pathways in human bronchial cells

    International Nuclear Information System (INIS)

    Carter, Charleata A.; Hamm, Jonathan T.

    2009-01-01

    Human bronchial cells are one of the first cell types exposed to environmental toxins. Toxins often activate nuclear factor-κB (NF-κB) and protein kinase C (PKC). We evaluated the hypothesis that cigarette smoke condensate (CSC), the particulate fraction of cigarette smoke, activates PKC-α and NF-κB, and concomitantly disrupts the F-actin cytoskeleton, induces apoptosis and alters cell function in BEAS-2B human bronchial epithelial cells. Compared to controls, exposure of BEAS-2B cells to doses of 30 μg/ml CSC significantly activated PKC-α, while CSC doses above 20 μg/ml CSC significantly activated NF-κB. As NF-κB was activated, cell number decreased. CSC treatment of BEAS-2B cells induced a decrease in cell size and an increase in cell surface extensions including filopodia and lamellipodia. CSC treatment of BEAS-2B cells induced F-actin rearrangement such that stress fibers were no longer prominent at the cell periphery and throughout the cells, but relocalized to perinuclear regions. Concurrently, CSC induced an increase in the focal adhesion protein vinculin at the cell periphery. CSC doses above 30 μg/ml induced a significant increase in apoptosis in BEAS-2B cells evidenced by an increase in activated caspase 3, an increase in mitochondrial mass and a decrease in mitochondrial membrane potential. As caspase 3 increased, cell number decreased. CSC doses above 30 μg/ml also induced significant concurrent changes in cell function including decreased cell spreading and motility. CSC initiates a signaling cascade in human bronchial epithelial cells involving PKC-α, NF-κB and caspase 3, and consequently decreases cell spreading and motility. These CSC-induced alterations in cell structure likely prevent cells from performing their normal function thereby contributing to smoke-induced diseases.

  13. Simultaneous characterization of cellular RNA structure and function with in-cell SHAPE-Seq.

    Science.gov (United States)

    Watters, Kyle E; Abbott, Timothy R; Lucks, Julius B

    2016-01-29

    Many non-coding RNAs form structures that interact with cellular machinery to control gene expression. A central goal of molecular and synthetic biology is to uncover design principles linking RNA structure to function to understand and engineer this relationship. Here we report a simple, high-throughput method called in-cell SHAPE-Seq that combines in-cell probing of RNA structure with a measurement of gene expression to simultaneously characterize RNA structure and function in bacterial cells. We use in-cell SHAPE-Seq to study the structure-function relationship of two RNA mechanisms that regulate translation in Escherichia coli. We find that nucleotides that participate in RNA-RNA interactions are highly accessible when their binding partner is absent and that changes in RNA structure due to RNA-RNA interactions can be quantitatively correlated to changes in gene expression. We also characterize the cellular structures of three endogenously expressed non-coding RNAs: 5S rRNA, RNase P and the btuB riboswitch. Finally, a comparison between in-cell and in vitro folded RNA structures revealed remarkable similarities for synthetic RNAs, but significant differences for RNAs that participate in complex cellular interactions. Thus, in-cell SHAPE-Seq represents an easily approachable tool for biologists and engineers to uncover relationships between sequence, structure and function of RNAs in the cell. © The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

  14. In-cell NMR spectroscopy of proteins inside Xenopus laevis oocytes

    International Nuclear Information System (INIS)

    Sakai, Tomomi; Tochio, Hidehito; Tenno, Takeshi; Ito, Yutaka; Kokubo, Tetsuro; Hiroaki, Hidekazu; Shirakawa, Masahiro

    2006-01-01

    In-cell NMR is an application of solution NMR that enables the investigation of protein conformations inside living cells. We have measured in-cell NMR spectra in oocytes from the African clawed frog Xenopus laevis. 15 N-labeled ubiquitin, its derivatives and calmodulin were injected into Xenopus oocytes and two-dimensional 1 H- 15 N correlation spectra of the proteins were obtained. While the spectrum of wild-type ubiquitin in oocytes had rather fewer cross-peaks compared to its in vitro spectrum, ubiquitin derivatives that are presumably unable to bind to ubiquitin-interacting proteins gave a markedly larger number of cross-peaks. This observation suggests that protein-protein interactions between ubiquitin and ubiquitin-interacting proteins may cause NMR signal broadening, and hence spoil the quality of the in-cell HSQC spectra. In addition, we observed the maturation of ubiquitin precursor derivative in living oocytes using the in-cell NMR technique. This process was partly inhibited by pre-addition of ubiquitin aldehyde, a specific inhibitor for ubiquitin C-terminal hydrolase (UCH). Our work demonstrates the potential usefulness of in-cell NMR with Xenopus oocytes for the investigation of protein conformations and functions under intracellular environmental conditions

  15. Final Report - "CO2 Sequestration in Cell Biomass of Chlorobium Thiosulfatophilum"

    Energy Technology Data Exchange (ETDEWEB)

    James L. Gaddy, PhD; Ching-Whan Ko, PhD

    2009-05-04

    . crunogena produces a reasonable return when treating a sour gas stream of 120 million SCFD containing 2.5 percent H2S. In this case, the primary source of revenue is from desulfurization of the gas stream. While the technology has significant application in sequestering carbon dioxide in cell biomass or single cell proten (SCP), perhaps the most immediate application is in desulfurizing LGNG or other gas streams. This biological approach is a viable economical alternative to existing hydrogen sulfide removal technology, and is not sensitive to the presence of hydrocarbons which act as catalyst poisons.

  16. A high-order particle-in-cell method for low density plasma flow and the simulation of gyrotron resonator devices

    International Nuclear Information System (INIS)

    Stock, Andreas

    2013-01-01

    Within this thesis a parallelized, transient, three-dimensional, high-order discontinuous Galerkin Particle-in-Cell solver is developed and used to simulate the resonant cavity of a gyrotron. The high-order discontinuous Galerkin approach - a Finite-Element type method - provides a fast and efficient algorithm to numerically solve Maxwell's equations used within this thesis. Besides its outstanding dissipation and dispersion properties, the discontinuous Galerkin approach easily allows for using unstructured grids, as required to simulate complex-shaped engineering devices. The discontinuous Galerkin approach approximates a wavelength with significantly less degrees of freedom compared to other methods, e.g. Finite Difference methods. Furthermore, the parallelization capabilities of the discontinuous Galerkin framework are excellent due to the very local dependencies between the elements. These properties are essential for the efficient numerical treatment of the Vlasov-Maxwell system with the Particle-in-Cell method. This system describes the self-consistent interaction of charged particles and the electromagnetic field. As central application within this thesis gyrotron resonators are simulated with the discontinuous Galerkin Particle-in-Cell method on high-performance-computers. The gyrotron is a high-power millimeter wave source, used for the electron cyclotron resonance heating of magnetically confined fusion plasma, e.g. in the Wendelstein 7-X experimental fusion-reactor. Compared to state-of-the-art simulation tools used for the design of gyrotron resonators the Particle-in-Cell method does not use any significant physically simplifications w.r.t. the modelling of the particle-field-interaction, the geometry and the wave-spectrum. Hence, it is the method of choice for validation of current simulation tools being restricted by these simplifications. So far, the Particle-in-Cell method was restricted to be used for demonstration calculations only, because

  17. A high-order particle-in-cell method for low density plasma flow and the simulation of gyrotron resonator devices

    Energy Technology Data Exchange (ETDEWEB)

    Stock, Andreas

    2013-04-26

    Within this thesis a parallelized, transient, three-dimensional, high-order discontinuous Galerkin Particle-in-Cell solver is developed and used to simulate the resonant cavity of a gyrotron. The high-order discontinuous Galerkin approach - a Finite-Element type method - provides a fast and efficient algorithm to numerically solve Maxwell's equations used within this thesis. Besides its outstanding dissipation and dispersion properties, the discontinuous Galerkin approach easily allows for using unstructured grids, as required to simulate complex-shaped engineering devices. The discontinuous Galerkin approach approximates a wavelength with significantly less degrees of freedom compared to other methods, e.g. Finite Difference methods. Furthermore, the parallelization capabilities of the discontinuous Galerkin framework are excellent due to the very local dependencies between the elements. These properties are essential for the efficient numerical treatment of the Vlasov-Maxwell system with the Particle-in-Cell method. This system describes the self-consistent interaction of charged particles and the electromagnetic field. As central application within this thesis gyrotron resonators are simulated with the discontinuous Galerkin Particle-in-Cell method on high-performance-computers. The gyrotron is a high-power millimeter wave source, used for the electron cyclotron resonance heating of magnetically confined fusion plasma, e.g. in the Wendelstein 7-X experimental fusion-reactor. Compared to state-of-the-art simulation tools used for the design of gyrotron resonators the Particle-in-Cell method does not use any significant physically simplifications w.r.t. the modelling of the particle-field-interaction, the geometry and the wave-spectrum. Hence, it is the method of choice for validation of current simulation tools being restricted by these simplifications. So far, the Particle-in-Cell method was restricted to be used for demonstration calculations only, because

  18. An in-cell NMR study of monitoring stress-induced increase of cytosolic Ca{sup 2+} concentration in HeLa cells

    Energy Technology Data Exchange (ETDEWEB)

    Hembram, Dambarudhar Shiba Sankar; Haremaki, Takahiro; Hamatsu, Jumpei; Inoue, Jin; Kamoshida, Hajime; Ikeya, Teppei; Mishima, Masaki [Department of Chemistry, Graduate School of Science and Engineering, Tokyo Metropolitan University, 1-1 Minami-Osawa, Hachioji-shi, Tokyo 192-0373 (Japan); Mikawa, Tsutomu [Cellular and Molecular Biology Unit, RIKEN Advanced Science Institute, Wako-shi, Saitama 351-0198 (Japan); Hayashi, Nobuhiro [Department of Life Science, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 B-1, Nagatsuda-chou, Midori-ku, Yokohama, Kanagawa 226-8501 (Japan); Shirakawa, Masahiro [Department of Molecular Engineering, Graduate School of Engineering, Kyoto University, Nishikyo-ku, Kyoto 615-8510 (Japan); Ito, Yutaka, E-mail: ito-yutaka@tmu.ac.jp [Department of Chemistry, Graduate School of Science and Engineering, Tokyo Metropolitan University, 1-1 Minami-Osawa, Hachioji-shi, Tokyo 192-0373 (Japan)

    2013-09-06

    proteins involved in the intracellular signalling systems. Our method provides a very useful tool for in situ monitoring of the “healthiness” of the cells in various in-cell NMR studies.

  19. Imaging lipid domains in cell membranes: the advent of super-resolution fluorescence microscopy

    Directory of Open Access Journals (Sweden)

    Dylan Myers Owen

    2013-12-01

    Full Text Available The lipid bilayer of model membranes, liposomes reconstituted from cell lipids, and plasma membrane vesicles and spheres can separate into two distinct liquid phases to yield lipid domains with liquid-ordered and liquid-disordered properties. These observations are the basis of the lipid raft hypothesis that postulates the existence of cholesterol-enriched ordered-phase lipid domains in cell membranes that could regulate protein mobility, localization and interaction. Here we review the evidence that nano-scaled lipid complexes and meso-scaled lipid domains exist in cell membranes and how new fluorescence microscopy techniques that overcome the diffraction limit provide new insights into lipid organization in cell membranes.

  20. Multi-grid Particle-in-cell Simulations of Plasma Microturbulence

    International Nuclear Information System (INIS)

    Lewandowski, J.L.V.

    2003-01-01

    A new scheme to accurately retain kinetic electron effects in particle-in-cell (PIC) simulations for the case of electrostatic drift waves is presented. The splitting scheme, which is based on exact separation between adiabatic and on adiabatic electron responses, is shown to yield more accurate linear growth rates than the standard df scheme. The linear and nonlinear elliptic problems that arise in the splitting scheme are solved using a multi-grid solver. The multi-grid particle-in-cell approach offers an attractive path, both from the physics and numerical points of view, to simulate kinetic electron dynamics in global toroidal plasmas

  1. Enzymatic Oxidation of Cholesterol: Properties and Functional Effects of Cholestenone in Cell Membranes

    DEFF Research Database (Denmark)

    Neuvonen, M.; Manna, M.; Mokkila, S.

    2014-01-01

    of cholestenone using simulations and cell biological experiments and assessed the functional effects of cholestenone in human cells. Atomistic simulations predicted that cholestenone reduces membrane order, undergoes faster flip-flop and desorbs more readily from membranes than cholesterol. In primary human...... fibroblasts, cholestenone was released from membranes to physiological extracellular acceptors more avidly than cholesterol, but without acceptors it remained in cells over a day. To address the functional effects of cholestenone, we studied fibroblast migration during wound healing. When cells were either...... similarly to control cells. Thus, cholesterol oxidation produces long-term functional effects in cells and these are in part due to the generated membrane active cholestenone....

  2. Particle-in-Cell Laser-Plasma Simulation on Xeon Phi Coprocessors

    OpenAIRE

    Surmin, I. A.; Bastrakov, S. I.; Efimenko, E. S.; Gonoskov, A. A.; Korzhimanov, A. V.; Meyerov, I. B.

    2015-01-01

    This paper concerns development of a high-performance implementation of the Particle-in-Cell method for plasma simulation on Intel Xeon Phi coprocessors. We discuss suitability of the method for Xeon Phi architecture and present our experience of porting and optimization of the existing parallel Particle-in-Cell code PICADOR. Direct porting with no code modification gives performance on Xeon Phi close to 8-core CPU on a benchmark problem with 50 particles per cell. We demonstrate step-by-step...

  3. Implementing particle-in-cell plasma simulation code on the BBN TC2000

    International Nuclear Information System (INIS)

    Sturtevant, J.E.; Maccabe, A.B.

    1990-01-01

    The BBN TC2000 is a multiple instruction, multiple data (MIMD) machine that combines a physically distributed memory with a logically shared memory programming environment using the unique Butterfly switch. Particle-In-Cell (PIC) plasma simulations model the interaction of charged particles with electric and magnetic fields. This paper describes the implementation of both a 1-D electrostatic and a 2 1/2-D electromagnetic PIC (particle-in-cell) plasma simulation code on a BBN TC2000. Performance is compared to implementations of the same code on the shared memory Sequent Balance and distributed memory Intel iPSC hypercube

  4. Thioridazine affects transcription of genes involved in cell wall biosynthesis in methicillin-resistant Staphylococcus aureus

    DEFF Research Database (Denmark)

    Bonde, Mette; Højland, Dorte Heidi; Kolmos, Hans Jørn

    2011-01-01

    have previously shown that the expression of some resistance genes is abolished after treatment with thioridazine and oxacillin. To further understand the mechanism underlying the reversal of resistance, we tested the expression of genes involved in antibiotic resistance and cell wall biosynthesis...... in response to thioridazine in combination with oxacillin. We observed that the oxacillin-induced expression of genes belonging to the VraSR regulon is reduced by the addition of thioridazine. The exclusion of such key factors involved in cell wall biosynthesis will most likely lead to a weakened cell wall...... reversal of resistance by thioridazine relies on decreased expression of specific genes involved in cell wall biosynthesis....

  5. System Budgets

    DEFF Research Database (Denmark)

    Jeppesen, Palle

    1996-01-01

    The lecture note is aimed at introducing system budgets for optical communication systems. It treats optical fiber communication systems (six generations), system design, bandwidth effects, other system impairments and optical amplifiers.......The lecture note is aimed at introducing system budgets for optical communication systems. It treats optical fiber communication systems (six generations), system design, bandwidth effects, other system impairments and optical amplifiers....

  6. Cell growth, intracellular calcium concentration and metabolic cooperation measured in cells exposed to 50 Hz electromagnetic fields

    International Nuclear Information System (INIS)

    Skauli, K.S.

    1996-08-01

    Colony-forming efficiency, DNA/protein and DNA/cell were measured in cells exposed to magnetic fields of 0.2 and 1 mT at a frequency of 50 Hz. Intracellular calcium concentrations were measured in cells exposed to 0.3 and 1 mT at 50 Hz. Metabolic cooperation was measured in cells exposed to 1 mT at 50 Hz. No significant effects of the fields were observed. 20 refs., 10 figs

  7. Involvement of Lgl and Mahjong/VprBP in cell competition.

    Directory of Open Access Journals (Sweden)

    Yoichiro Tamori

    2010-07-01

    Full Text Available During the initial stages of carcinogenesis, transformation events occur in a single cell within an epithelial monolayer. However, it remains unknown what happens at the interface between normal and transformed epithelial cells during this process. In Drosophila, it has been recently shown that normal and transformed cells compete with each other for survival in an epithelial tissue; however the molecular mechanisms whereby "loser cells" undergo apoptosis are not clearly understood. Lgl (lethal giant larvae is a tumor suppressor protein and plays a crucial role in oncogenesis in flies and mammals. Here we have examined the involvement of Lgl in cell competition and shown that a novel Lgl-binding protein is involved in Lgl-mediated cell competition. Using biochemical immunoprecipitation methods, we first identified Mahjong as a novel binding partner of Lgl in both flies and mammals. In Drosophila, Mahjong is an essential gene, but zygotic mahjong mutants (mahj(-/- do not have obvious patterning defects during embryonic or larval development. However, mahj(-/- cells undergo apoptosis when surrounded by wild-type cells in the wing disc epithelium. Importantly, comparable phenomena also occur in Mahjong-knockdown mammalian cells; Mahjong-knockdown Madin-Darby canine kidney epithelial cells undergo apoptosis, only when surrounded by non-transformed cells. Similarly, apoptosis of lgl(-/- cells is induced when they are surrounded by wild-type cells in Drosophila wing discs. Phosphorylation of the c-Jun N-terminal kinase (JNK is increased in mahj(-/- or lgl(-/- mutant cells, and expression of Puckered (Puc, an inhibitor of the JNK pathway, suppresses apoptosis of these mutant cells surrounded by wild-type cells, suggesting that the JNK pathway is involved in mahj- or lgl-mediated cell competition. Finally, we have shown that overexpression of Mahj in lgl(-/- cells strongly suppresses JNK activation and blocks apoptosis of lgl(-/- cells in the wild

  8. A role for BELLRINGER in cell wall development is supported by loss-of-function phenotypes

    Directory of Open Access Journals (Sweden)

    Etchells J

    2012-11-01

    Full Text Available Abstract Background Homeodomain transcription factors play critical roles in metazoan development. BELLRINGER (BLR, one such transcription factor, is involved in diverse developmental processes in Arabidopsis, acting in vascular differentiation, phyllotaxy, flower and fruit development. BLR also has a redundant role in meristem maintenance. Cell wall remodelling underpins many of these processes, and BLR has recently been shown to regulate expression of PECTIN METHYL-ESTERASE 5 (PME5, a cell wall modifying enzyme in control of phyllotaxy. We have further explored the role of BLR in plant development by analysing phenotypes and gene expression in a series of plants over-expressing BLR, and generating combinatorial mutants with blr, brevipedicellus (bp, a member of the KNOX1 family of transcription factors that has previously been shown to interact with blr, and the homeodomain transcription factor revoluta (rev, required for radial patterning of the stem. Results Plants over-expressing BLR exhibited a wide range of phenotypes. Some were defective in cell size and demonstrated misregulation of genes predominantly affecting cell wall development. Other lines with more extreme phenotypes failed to generate lateral organs, consistent with BLR repressing transcription in the shoot apex. Cell wall dynamics are also affected in blr mutant plants, and BLR has previously been shown to regulate vascular development in conjunction with BP. We found that when bp and blr were combined with rev, a set of defects was observed that were distinct from those of bp blr lines. In these triple mutants xylem development was most strikingly affected, resulting in an almost complete lack of vessels and xylem parenchyma with secondary thickening. Conclusions Our data support a role for BLR in ordering the shoot apex and, in conjunction with BP and REV, playing a part in determining the composition and organisation of the vascular system. Microarray analysis strongly

  9. Development of 2D particle-in-cell code to simulate high current, low ...

    Indian Academy of Sciences (India)

    Abstract. A code for 2D space-charge dominated beam dynamics study in beam trans- port lines is developed. The code is used for particle-in-cell (PIC) simulation of z-uniform beam in a channel containing solenoids and drift space. It can also simulate a transport line where quadrupoles are used for focusing the beam.

  10. Dynamic Pedagogy for Effective Training of Youths in Cell Phone Maintenance

    Science.gov (United States)

    Ogbuanya, T. C.; Jimoh, Bakare

    2015-01-01

    The study determined dynamic pedagogies for effective training of youths in cell phone maintenance. The study was conducted in Enugu State of Nigeria. Four research questions were developed while four null hypotheses formulated were tested at 0.05 level of significance. A survey research design was adopted for the study. The population for the…

  11. Identification of miRNA targets with stable isotope labeling by amino acids in cell culture

    DEFF Research Database (Denmark)

    Vinther, Jeppe; Hedegaard, Mads Marquardt; Gardner, Paul Phillip

    2006-01-01

    miRNAs are small noncoding RNAs that regulate gene expression. We have used stable isotope labeling by amino acids in cell culture (SILAC) to investigate the effect of miRNA-1 on the HeLa cell proteome. Expression of 12 out of 504 investigated proteins was repressed by miRNA-1 transfection...

  12. A 31-residue peptide induces aggregation of tau's microtubule-binding region in cells

    Science.gov (United States)

    Stöhr, Jan; Wu, Haifan; Nick, Mimi; Wu, Yibing; Bhate, Manasi; Condello, Carlo; Johnson, Noah; Rodgers, Jeffrey; Lemmin, Thomas; Acharya, Srabasti; Becker, Julia; Robinson, Kathleen; Kelly, Mark J. S.; Gai, Feng; Stubbs, Gerald; Prusiner, Stanley B.; Degrado, William F.

    2017-09-01

    The self-propagation of misfolded conformations of tau underlies neurodegenerative diseases, including Alzheimer's. There is considerable interest in discovering the minimal sequence and active conformational nucleus that defines this self-propagating event. The microtubule-binding region, spanning residues 244-372, reproduces much of the aggregation behaviour of tau in cells and animal models. Further dissection of the amyloid-forming region to a hexapeptide from the third microtubule-binding repeat resulted in a peptide that rapidly forms fibrils in vitro. We show that this peptide lacks the ability to seed aggregation of tau244-372 in cells. However, as the hexapeptide is gradually extended to 31 residues, the peptides aggregate more slowly and gain potent activity to induce aggregation of tau244-372 in cells. X-ray fibre diffraction, hydrogen-deuterium exchange and solid-state NMR studies map the beta-forming region to a 25-residue sequence. Thus, the nucleus for self-propagating aggregation of tau244-372 in cells is packaged in a remarkably small peptide.

  13. Radiation-induced irreparable heritable changes in cells promoting their tumoral transformation

    International Nuclear Information System (INIS)

    Kuzin, A.M.; Vagabova, M.Eh.; Yurov, S.S.

    1988-01-01

    In experiments with model plant tumors (Kalanchoe-ti plasmid Agrobat. tumefaciens C-58D) it was shown that exposure of the recepient plant to low-level γ-radiation of Gy induced changes in cells that were not repaired over two months promoting tumoral transformations in them. Those changes were shown to persist in the offspring of the exposed somatic cells

  14. Insights into the function and dysfunction of α-synuclein in cells

    NARCIS (Netherlands)

    Raiss, C.C.

    2015-01-01

    This thesis sheds light on the function and dysfunction of the protein α-synuclein (α-S) in the test tube and in cells and ultimately its possible involvement in Parkinson’s disease (PD). Following the introduction in Chapter 1, Chapters 2 and 3 concentrate on the investigation of the interaction

  15. Ecdysone Receptor-based Singular Gene Switches for Regulated Transgene Expression in Cells and Adult Rodent Tissues

    Directory of Open Access Journals (Sweden)

    Seoghyun Lee

    2016-01-01

    Full Text Available Controlled gene expression is an indispensable technique in biomedical research. Here, we report a convenient, straightforward, and reliable way to induce expression of a gene of interest with negligible background expression compared to the most widely used tetracycline (Tet-regulated system. Exploiting a Drosophila ecdysone receptor (EcR-based gene regulatory system, we generated nonviral and adenoviral singular vectors designated as pEUI(+ and pENTR-EUI, respectively, which contain all the required elements to guarantee regulated transgene expression (GAL4-miniVP16-EcR, termed GvEcR hereafter, and 10 tandem repeats of an upstream activation sequence promoter followed by a multiple cloning site. Through the transient and stable transfection of mammalian cell lines with reporter genes, we validated that tebufenozide, an ecdysone agonist, reversibly induced gene expression, in a dose- and time-dependent manner, with negligible background expression. In addition, we created an adenovirus derived from the pENTR-EUI vector that readily infected not only cultured cells but also rodent tissues and was sensitive to tebufenozide treatment for regulated transgene expression. These results suggest that EcR-based singular gene regulatory switches would be convenient tools for the induction of gene expression in cells and tissues in a tightly controlled fashion.

  16. Efficient particle-in-cell simulation of auroral plasma phenomena using a CUDA enabled graphics processing unit

    Science.gov (United States)

    Sewell, Stephen

    This thesis introduces a software framework that effectively utilizes low-cost commercially available Graphic Processing Units (GPUs) to simulate complex scientific plasma phenomena that are modeled using the Particle-In-Cell (PIC) paradigm. The software framework that was developed conforms to the Compute Unified Device Architecture (CUDA), a standard for general purpose graphic processing that was introduced by NVIDIA Corporation. This framework has been verified for correctness and applied to advance the state of understanding of the electromagnetic aspects of the development of the Aurora Borealis and Aurora Australis. For each phase of the PIC methodology, this research has identified one or more methods to exploit the problem's natural parallelism and effectively map it for execution on the graphic processing unit and its host processor. The sources of overhead that can reduce the effectiveness of parallelization for each of these methods have also been identified. One of the novel aspects of this research was the utilization of particle sorting during the grid interpolation phase. The final representation resulted in simulations that executed about 38 times faster than simulations that were run on a single-core general-purpose processing system. The scalability of this framework to larger problem sizes and future generation systems has also been investigated.

  17. Ventilation systems

    International Nuclear Information System (INIS)

    Gossler

    1980-01-01

    The present paper deals with - controlled area ventilation systems - ventilation systems for switchgear-building and control-room - other ventilation systems for safety equipments - service systems for ventilation systems. (orig./RW)

  18. Role of human pulmonary fibroblast-derived MCP-1 in cell activation and migration in experimental silicosis

    Energy Technology Data Exchange (ETDEWEB)

    Liu, Xueting [Department of Physiology, Medical School of Southeast University, Nanjing, Jiangsu 210009 (China); Fang, Shencun [Nine Department of Respiratory Medicine, Nanjing Chest Hospital, Nanjing, Jiangsu 210029 (China); Liu, Haijun [Neurobiology Laboratory, New Drug Screening Centre, China Pharmaceutical University, Nanjing, Jiangsu 210009 (China); Wang, Xingang; Dai, Xiaoniu; Yin, Qing; Yun, Tianwei [Department of Physiology, Medical School of Southeast University, Nanjing, Jiangsu 210009 (China); Wang, Wei; Zhang, Yingming [Nine Department of Respiratory Medicine, Nanjing Chest Hospital, Nanjing, Jiangsu 210029 (China); Liao, Hong [Neurobiology Laboratory, New Drug Screening Centre, China Pharmaceutical University, Nanjing, Jiangsu 210009 (China); Zhang, Wei [Department of Physiology, Medical School of Southeast University, Nanjing, Jiangsu 210009 (China); Yao, Honghong [Department of Pharmacology, Medical School of Southeast University, Nanjing, Jiangsu 210009 (China); Chao, Jie, E-mail: chaojie@seu.edu.cn [Department of Physiology, Medical School of Southeast University, Nanjing, Jiangsu 210009 (China)

    2015-10-15

    Background: Silicosis is a systemic disease caused by inhaling silicon dioxide (SiO{sub 2}). Phagocytosis of SiO{sub 2} in the lung initiates an inflammatory cascade that results in fibroblast proliferation and migration and subsequent fibrosis. Clinical evidence indicates that the activation of alveolar macrophages by SiO{sub 2} produces rapid and sustained inflammation that is characterized by the generation of monocyte chemotactic protein 1 (MCP-1), which induces fibrosis. Pulmonary fibroblast-derived MCP-1 may play a critical role in fibroblast proliferation and migration. Methods and results: Experiments using primary cultured adult human pulmonary fibroblasts (HPF-a) demonstrated the following results: 1) SiO{sub 2} treatment resulted in the rapid and sustained induction of MCP-1 as well as the elevation of the CC chemokine receptor type 2 (CCR2) protein levels; 2) pretreatment of HPF-a with RS-102895, a specific CCR2 inhibitor, abolished the SiO{sub 2}-induced increase in cell activation and migration in both 2D and 3D culture systems; and 3) RNA interference targeting CCR2 prevented the SiO{sub 2}-induced increase in cell migration. Conclusion: These data demonstrated that the up-regulation of pulmonary fibroblast-derived MCP-1 is involved in pulmonary fibroblast migration induced by SiO{sub 2}. CCR2 was also up-regulated in response to SiO{sub 2}, and this up-regulation facilitated the effect of MCP-1 on fibroblasts. Our study deciphered the link between fibroblast-derived MCP-1 and SiO{sub 2}-induced cell migration. This finding provides novel insight into the potential of MCP-1 in the development of novel therapeutic strategies for silicosis. - Highlights: • Role of pulmonary fibroblast-derived MCP-1 in experimental silicosis was studied. • SiO{sub 2} induced MCP-1 release from cultured human pulmonary fibroblast (HPF-a). • SiO{sub 2} directly activated HPF-a via the MCP-1/CCR2 pathway. • SiO{sub 2} increased HPF-a migration in both 2D and 3D

  19. Role of human pulmonary fibroblast-derived MCP-1 in cell activation and migration in experimental silicosis

    International Nuclear Information System (INIS)

    Liu, Xueting; Fang, Shencun; Liu, Haijun; Wang, Xingang; Dai, Xiaoniu; Yin, Qing; Yun, Tianwei; Wang, Wei; Zhang, Yingming; Liao, Hong; Zhang, Wei; Yao, Honghong; Chao, Jie

    2015-01-01

    Background: Silicosis is a systemic disease caused by inhaling silicon dioxide (SiO 2 ). Phagocytosis of SiO 2 in the lung initiates an inflammatory cascade that results in fibroblast proliferation and migration and subsequent fibrosis. Clinical evidence indicates that the activation of alveolar macrophages by SiO 2 produces rapid and sustained inflammation that is characterized by the generation of monocyte chemotactic protein 1 (MCP-1), which induces fibrosis. Pulmonary fibroblast-derived MCP-1 may play a critical role in fibroblast proliferation and migration. Methods and results: Experiments using primary cultured adult human pulmonary fibroblasts (HPF-a) demonstrated the following results: 1) SiO 2 treatment resulted in the rapid and sustained induction of MCP-1 as well as the elevation of the CC chemokine receptor type 2 (CCR2) protein levels; 2) pretreatment of HPF-a with RS-102895, a specific CCR2 inhibitor, abolished the SiO 2 -induced increase in cell activation and migration in both 2D and 3D culture systems; and 3) RNA interference targeting CCR2 prevented the SiO 2 -induced increase in cell migration. Conclusion: These data demonstrated that the up-regulation of pulmonary fibroblast-derived MCP-1 is involved in pulmonary fibroblast migration induced by SiO 2 . CCR2 was also up-regulated in response to SiO 2 , and this up-regulation facilitated the effect of MCP-1 on fibroblasts. Our study deciphered the link between fibroblast-derived MCP-1 and SiO 2 -induced cell migration. This finding provides novel insight into the potential of MCP-1 in the development of novel therapeutic strategies for silicosis. - Highlights: • Role of pulmonary fibroblast-derived MCP-1 in experimental silicosis was studied. • SiO 2 induced MCP-1 release from cultured human pulmonary fibroblast (HPF-a). • SiO 2 directly activated HPF-a via the MCP-1/CCR2 pathway. • SiO 2 increased HPF-a migration in both 2D and 3D model via the MCP-1/CCR2 pathway. • RNA-i of MCP-1/CCR2

  20. Evaluation of diel patterns of relative changes in cell turgor of tomato plants using leaf patch clamp pressure probes

    NARCIS (Netherlands)

    Lee, K.M.; Driever, S.M.; Heuvelink, E.; Rüger, S.; Zimmermann, U.; Gelder, de A.; Marcelis, L.F.M.

    2012-01-01

    Relative changes in cell turgor of leaves of well-watered tomato plants were evaluated using the leaf patch clamp pressure probe (LPCP) under dynamic greenhouse climate conditions. Leaf patch clamp pressure changes, a measure for relative changes in cell turgor, were monitored at three different

  1. 6-shogaol-rich extract from ginger up-regulates the antioxidant defense systems in cells and mice.

    Science.gov (United States)

    Bak, Min-Ji; Ok, Seon; Jun, Mira; Jeong, Woo-Sik

    2012-07-04

    The rhizome of ginger (Zingiber officinale Roscoe) is known to have several bioactive compounds including gingerols and shogaols which possess beneficial health properties such as anti-inflammatory and chemopreventive effects. Based on recent observations that 6-shogaol may have more potent bioactivity than 6-gingerol, we obtained a 6-shogaol-rich extract from ginger and examined its effects on the nuclear factor E2-related factor2 (Nrf2)/antioxidant response element (ARE) pathway in vitro and in vivo. 6-Shogaol-rich extract was produced by extracting ginger powder with 95% ethanol at 80 °C after drying at 80 °C (GEE8080). GEE8080 contained over 6-fold more 6-shogaol compared to the room temperature extract (GEE80RT). In HepG2 cells, GEE8080 displayed much stronger inductions of ARE-reporter gene activity and Nrf2 expression than GEE80RT. GEE8080 stimulated phosphorylations of mitogen-activated protein kinases (MAPKs) such as ERK, JNK, and p38. Moreover, the GEE8080-induced expressions of Nrf2 and HO-1 were attenuated by treatments of SB202190 (a p38 specific inhibitor) and LY294002 (an Akt specific inhibitor). In a mouse model, the GEE8080 decreased the diethylnitrosamine (DEN)-mediated elevations of serum aspartate transaminase and alanine transaminase as well as the DEN-induced hepatic lipid peroxidation. Inductions of Nrf2 and HO-1 by GEE8080 were also confirmed in the mice. In addition, the administration of GEE8080 to the mice also restored the DEN-reduced activity and protein expression of hepatic antioxidant enzymes such as superoxide dismutase, glutathione peroxidase and catalase. In conclusion, GEE8080, a 6-shogaol-rich ginger extract, may enhance antioxidant defense mechanism through the induction of Nrf2 and HO-1 regulated by p38 MAPK and PI3k/Akt pathway in vitro and in vivo.

  2. 6-Shogaol-Rich Extract from Ginger Up-Regulates the Antioxidant Defense Systems in Cells and Mice

    Directory of Open Access Journals (Sweden)

    Mira Jun

    2012-07-01

    Full Text Available The rhizome of ginger (Zingiber officinale Roscoe is known to have several bioactive compounds including gingerols and shogaols which possess beneficial health properties such as anti-inflammatory and chemopreventive effects. Based on recent observations that 6-shogaol may have more potent bioactivity than 6-gingerol, we obtained a 6-shogaol-rich extract from ginger and examined its effects on the nuclear factor E2-related factor2 (Nrf2/antioxidant response element (ARE pathway in vitro and in vivo. 6-Shogaol-rich extract was produced by extracting ginger powder with 95% ethanol at 80 °C after drying at 80 °C (GEE8080. GEE8080 contained over 6-fold more 6-shogaol compared to the room temperature extract (GEE80RT. In HepG2 cells, GEE8080 displayed much stronger inductions of ARE-reporter gene activity and Nrf2 expression than GEE80RT. GEE8080 stimulated phosphorylations of mitogen-activated protein kinases (MAPKs such as ERK, JNK, and p38. Moreover, the GEE8080-induced expressions of Nrf2 and HO-1 were attenuated by treatments of SB202190 (a p38 specific inhibitor and LY294002 (an Akt specific inhibitor. In a mouse model, the GEE8080 decreased the diethylnitrosamine (DEN-mediated elevations of serum aspartate transaminase and alanine transaminase as well as the DEN-induced hepatic lipid peroxidation. Inductions of Nrf2 and HO-1 by GEE8080 were also confirmed in the mice. In addition, the administration of GEE8080 to the mice also restored the DEN-reduced activity and protein expression of hepatic antioxidant enzymes such as superoxide dismutase, glutathione peroxidase and catalase. In conclusion, GEE8080, a 6-shogaol-rich ginger extract, may enhance antioxidant defense mechanism through the induction of Nrf2 and HO-1 regulated by p38 MAPK and PI3k/Akt pathway in vitro and in vivo.

  3. 6-Shogaol-Rich Extract from Ginger Up-Regulates the Antioxidant Defense Systems in Cells and Mice

    OpenAIRE

    Bak, Min-Ji; Ok, Seon; Jun, Mira; Jeong, Woo-Sik

    2012-01-01

    The rhizome of ginger (Zingiber officinale Roscoe) is known to have several bioactive compounds including gingerols and shogaols which possess beneficial health properties such as anti-inflammatory and chemopreventive effects. Based on recent observations that 6-shogaol may have more potent bioactivity than 6-gingerol, we obtained a 6-shogaol-rich extract from ginger and examined its effects on the nuclear factor E2-related factor2 (Nrf2)/antioxidant response element (ARE...

  4. Expression and regulation of CYP17A1 and 3β-hydroxysteroid dehydrogenase in cells of the nervous system

    DEFF Research Database (Denmark)

    Emanuelsson, I; Almokhtar, M; Wikvall, K

    2018-01-01

    investigation we studied the expression of these enzymes in cultured primary rat astrocytes, in neuron-enriched cells from rat cerebral cortex and in human neuroblastoma SH-SY5Y cells, a cell line often used as an in vitro model of neuronal function and differentiation. As part of this study we also examined...

  5. Atomic force microscopy: Unraveling the fundamental principles governing secretion and membrane fusion in cells

    International Nuclear Information System (INIS)

    Jena, Bhanu P.

    2009-01-01

    The story of cell secretion and membrane fusion is as old as life itself. Without these fundamental cellular processes known to occur in yeast to humans, life would cease to exist. In the last 15 years, primarily using the atomic force microscope, a detailed understanding of the molecular process and of the molecular machinery and mechanism of secretion and membrane fusion in cells has come to light. This has led to a paradigm shift in our understanding of the underlying mechanism of cell secretion. The journey leading to the discovery of a new cellular structure the 'porosome',-the universal secretory machinery in cells, and the contributions of the AFM in our understanding of the general molecular machinery and mechanism of cell secretion and membrane fusion, is briefly discussed in this article.

  6. Particle-in-cell simulations of plasma accelerators and electron-neutral collisions

    Directory of Open Access Journals (Sweden)

    David L. Bruhwiler

    2001-10-01

    Full Text Available We present 2D simulations of both beam-driven and laser-driven plasma wakefield accelerators, using the object-oriented particle-in-cell code XOOPIC, which is time explicit, fully electromagnetic, and capable of running on massively parallel supercomputers. Simulations of laser-driven wakefields with low \\(∼10^{16} W/cm^{2}\\ and high \\(∼10^{18} W/cm^{2}\\ peak intensity laser pulses are conducted in slab geometry, showing agreement with theory and fluid simulations. Simulations of the E-157 beam wakefield experiment at the Stanford Linear Accelerator Center, in which a 30 GeV electron beam passes through 1 m of preionized lithium plasma, are conducted in cylindrical geometry, obtaining good agreement with previous work. We briefly describe some of the more significant modifications to XOOPIC required by this work, and summarize the issues relevant to modeling relativistic electron-neutral collisions in a particle-in-cell code.

  7. A Global Map of Lipid-Binding Proteins and Their Ligandability in Cells.

    Science.gov (United States)

    Niphakis, Micah J; Lum, Kenneth M; Cognetta, Armand B; Correia, Bruno E; Ichu, Taka-Aki; Olucha, Jose; Brown, Steven J; Kundu, Soumajit; Piscitelli, Fabiana; Rosen, Hugh; Cravatt, Benjamin F

    2015-06-18

    Lipids play central roles in physiology and disease, where their structural, metabolic, and signaling functions often arise from interactions with proteins. Here, we describe a set of lipid-based chemical proteomic probes and their global interaction map in mammalian cells. These interactions involve hundreds of proteins from diverse functional classes and frequently occur at sites of drug action. We determine the target profiles for several drugs across the lipid-interaction proteome, revealing that its ligandable content extends far beyond traditionally defined categories of druggable proteins. In further support of this finding, we describe a selective ligand for the lipid-binding protein nucleobindin-1 (NUCB1) and show that this compound perturbs the hydrolytic and oxidative metabolism of endocannabinoids in cells. The described chemical proteomic platform thus provides an integrated path to both discover and pharmacologically characterize a wide range of proteins that participate in lipid pathways in cells. Copyright © 2015 Elsevier Inc. All rights reserved.

  8. Advanced particle-in-cell simulation techniques for modeling the Lockheed Martin Compact Fusion Reactor

    Science.gov (United States)

    Welch, Dale; Font, Gabriel; Mitchell, Robert; Rose, David

    2017-10-01

    We report on particle-in-cell developments of the study of the Compact Fusion Reactor. Millisecond, two and three-dimensional simulations (cubic meter volume) of confinement and neutral beam heating of the magnetic confinement device requires accurate representation of the complex orbits, near perfect energy conservation, and significant computational power. In order to determine initial plasma fill and neutral beam heating, these simulations include ionization, elastic and charge exchange hydrogen reactions. To this end, we are pursuing fast electromagnetic kinetic modeling algorithms including a two implicit techniques and a hybrid quasi-neutral algorithm with kinetic ions. The kinetic modeling includes use of the Poisson-corrected direct implicit, magnetic implicit, as well as second-order cloud-in-cell techniques. The hybrid algorithm, ignoring electron inertial effects, is two orders of magnitude faster than kinetic but not as accurate with respect to confinement. The advantages and disadvantages of these techniques will be presented. Funded by Lockheed Martin.

  9. Electromagnetic particle in cell modeling of the plasma focus: Current sheath formation and lift off

    International Nuclear Information System (INIS)

    Seng, Y. S.; Lee, P.; Rawat, R. S.

    2014-01-01

    The shaping and formation of the current sheath takes place in the breakdown phase of a plasma focus device and critically controls the device performance. Electrostatic particle in cell codes, with magnetic effects ignored, have been used to model the breakdown phase. This Letter reports the successful development and implementation of an electromagnetic particle in cell (EMPIC) code, including magnetic effects self-consistently, to simulate the breakdown phase; from the ionization, localization and gliding discharge along the insulator to the time instant of current sheath lift off. The magnetic field was found to be appreciable from the time the current sheath came into contact with the anode with increased local current, initiating the voltage breakdown of the device as a result

  10. Application of the particle-in-cell method in propagation calculations

    International Nuclear Information System (INIS)

    Voelz, E.

    1979-01-01

    The Particle-in-Cell-Method that is capable of calculating the spreading of a plume in the atmosphere under instationary and inhomogeneous conditions, has a systematical advantage over the steady state Gaussian plume model usually used. Especially the fixed-point concentration time integral is calculated realistically instead of the locally integrated concentration at a constant time as is done in the plume model. Inaccuracies due to the computational techniques may be avoided in this way. On the other hand, at first the turbulent diffusion coefficients that describe the diffusion in the particle-in-cell method, must be prepared for all diffusion types. Thereby the diffusion coefficients can be seen to be mainly deduced in the steady state. This is one reason why they cannot be used in an optimal sense in a model that actually works instationary. (orig.) [de

  11. In-cell thermodynamics and a new role for protein surfaces.

    Science.gov (United States)

    Smith, Austin E; Zhou, Larry Z; Gorensek, Annelise H; Senske, Michael; Pielak, Gary J

    2016-02-16

    There is abundant, physiologically relevant knowledge about protein cores; they are hydrophobic, exquisitely well packed, and nearly all hydrogen bonds are satisfied. An equivalent understanding of protein surfaces has remained elusive because proteins are almost exclusively studied in vitro in simple aqueous solutions. Here, we establish the essential physiological roles played by protein surfaces by measuring the equilibrium thermodynamics and kinetics of protein folding in the complex environment of living Escherichia coli cells, and under physiologically relevant in vitro conditions. Fluorine NMR data on the 7-kDa globular N-terminal SH3 domain of Drosophila signal transduction protein drk (SH3) show that charge-charge interactions are fundamental to protein stability and folding kinetics in cells. Our results contradict predictions from accepted theories of macromolecular crowding and show that cosolutes commonly used to mimic the cellular interior do not yield physiologically relevant information. As such, we provide the foundation for a complete picture of protein chemistry in cells.

  12. A Thiopurine Drug Inhibits West Nile Virus Production in Cell Culture, but Not in Mice

    OpenAIRE

    Lim, Pei-Yin; Keating, Julie A.; Hoover, Spencer; Striker, Rob; Bernard, Kristen A.

    2011-01-01

    Many viruses within the Flavivirus genus cause significant disease in humans; however, effective antivirals against these viruses are not currently available. We have previously shown that a thiopurine drug, 6-methylmercaptopurine riboside (6MMPr), inhibits replication of distantly related viruses within the Flaviviridae family in cell culture, including bovine viral diarrhea virus and hepatitis C virus replicon. Here we further examined the potential antiviral effect of 6MMPr on several dive...

  13. Swelling-activated ion channels: functional regulation in cell-swelling, proliferation and apoptosis

    DEFF Research Database (Denmark)

    Stutzin, A; Hoffmann, E K

    2006-01-01

    Cell volume regulation is one of the most fundamental homeostatic mechanisms and essential for normal cellular function. At the same time, however, many physiological mechanisms are associated with regulatory changes in cell size meaning that the set point for cell volume regulation is under phys...... as key players in the maintenance of normal steady-state cell volume, with particular emphasis on the intracellular signalling pathways responsible for their regulation during hypotonic stress, cell proliferation and apoptosis....

  14. Fractional variational problems and particle in cell gyrokinetic simulations with fuzzy logic approach for tokamaks

    Directory of Open Access Journals (Sweden)

    Rastović Danilo

    2009-01-01

    Full Text Available In earlier Rastovic's papers [1] and [2], the effort was given to analyze the stochastic control of tokamaks. In this paper, the deterministic control of tokamak turbulence is investigated via fractional variational calculus, particle in cell simulations, and fuzzy logic methods. Fractional integrals can be considered as approximations of integrals on fractals. The turbulent media could be of the fractal structure and the corresponding equations should be changed to include the fractal features of the media.

  15. Particle-In-Cell/Monte Carlo Simulation of Ion Back Bombardment in Photoinjectors

    International Nuclear Information System (INIS)

    Qiang, Ji; Corlett, John; Staples, John

    2009-01-01

    In this paper, we report on studies of ion back bombardment in high average current dc and rf photoinjectors using a particle-in-cell/Monte Carlo method. Using H 2 ion as an example, we observed that the ion density and energy deposition on the photocathode in rf guns are order of magnitude lower than that in a dc gun. A higher rf frequency helps mitigate the ion back bombardment of the cathode in rf guns

  16. Radiation hardening and irradiation testing of in-cell electronics for MA23/APM

    International Nuclear Information System (INIS)

    Friant, A.

    1988-09-01

    We relate briefly the radiation hardening method used to guarantee a gamma resistance of 10 Mrad for the whole electronic equipment associated with the slave arm of MA23 M servomanipulator which will be set up in cell 404 in Marcoule (APM). We describe the radiation testing of electronic devices and of the various subsystems designed by the D. LETI groups involved in the MA23/APM project

  17. Cholesterol metabolism: use of D2O for determination of synthesis rate in cell culture

    International Nuclear Information System (INIS)

    Esterman, A.L.; Cohen, B.I.; Javitt, N.B.

    1985-01-01

    Cholesterol synthesis in cell culture in the presence of D 2 O yields a spectrum of enriched molecules having a relative abundance that indicates random substitution of deuterium for hydrogen. Quantitation of the absolute rate of cholesterol synthesis is obtained by isotope ratio mass spectrometry. Mevinolin and 26-hydroxycholesterol both decrease cholesterol synthesis rate but have a discordant effect on HMG-CoA reductase activity

  18. Chemoproteomics Reveals Chemical Diversity and Dynamics of 4-Oxo-2-nonenal Modifications in Cells.

    Science.gov (United States)

    Sun, Rui; Fu, Ling; Liu, Keke; Tian, Caiping; Yang, Yong; Tallman, Keri A; Porter, Ned A; Liebler, Daniel C; Yang, Jing

    2017-10-01

    4-Oxo-2-nonenal (ONE) derived from lipid peroxidation modifies nucleophiles and transduces redox signaling by its reactions with proteins. However, the molecular interactions between ONE and complex proteomes and their dynamics in situ remain largely unknown. Here we describe a quantitative chemoproteomic analysis of protein adduction by ONE in cells, in which the cellular target profile of ONE is mimicked by its alkynyl surrogate. The analyses reveal four types of ONE-derived modifications in cells, including ketoamide and Schiff-base adducts to lysine, Michael adducts to cysteine, and a novel pyrrole adduct to cysteine. ONE-derived adducts co-localize and exhibit crosstalk with many histone marks and redox sensitive sites. All four types of modifications derived from ONE can be reversed site-specifically in cells. Taken together, our study provides much-needed mechanistic insights into the cellular signaling and potential toxicities associated with this important lipid derived electrophile. © 2017 by The American Society for Biochemistry and Molecular Biology, Inc.

  19. Introduction to the Minireview Series on Modern Technologies for In-cell Biochemistry.

    Science.gov (United States)

    Lutsenko, Svetlana

    2016-02-19

    The last decade has seen enormous progress in the exploration and understanding of the behavior of molecules in their natural cellular environments at increasingly high spatial and temporal resolution. Advances in microscopy and the development of new fluorescent reagents as well as genetic editing techniques have enabled quantitative analysis of protein interactions, intracellular trafficking, metabolic changes, and signaling. Modern biochemistry now faces new and exciting challenges. Can traditionally "in vitro" experiments, e.g. analysis of protein folding and conformational transitions, be done in cells? Can the structure and behavior of endogenous and/or non-tagged recombinant proteins be analyzed and altered within the cell or in cellular compartments? How can molecules and their actions be studied mechanistically in tissues and organs? Is personalized cellular biochemistry a reality? This thematic series summarizes recent studies that illustrate some first steps toward successfully answering these modern biochemical questions. The first minireview focuses on utilization of three-dimensional primary enteroids and organoids for mechanistic studies of intestinal biology with molecular resolution. The second minireview describes application of single chain antibodies (nanobodies) for monitoring and regulating protein dynamics in vitro and in cells. The third minireview highlights advances in using NMR spectroscopy for analysis of protein folding and assembly in cells. © 2016 by The American Society for Biochemistry and Molecular Biology, Inc.

  20. Compensation of Wave-Induced Motion and Force Phenomena for Ship-Based High Performance Robotic and Human Amplifying Systems

    Energy Technology Data Exchange (ETDEWEB)

    Love, LJL

    2003-09-24

    The decrease in manpower and increase in material handling needs on many Naval vessels provides the motivation to explore the modeling and control of Naval robotic and robotic assistive devices. This report addresses the design, modeling, control and analysis of position and force controlled robotic systems operating on the deck of a moving ship. First we provide background information that quantifies the motion of the ship, both in terms of frequency and amplitude. We then formulate the motion of the ship in terms of homogeneous transforms. This transformation provides a link between the motion of the ship and the base of a manipulator. We model the kinematics of a manipulator as a serial extension of the ship motion. We then show how to use these transforms to formulate the kinetic and potential energy of a general, multi-degree of freedom manipulator moving on a ship. As a demonstration, we consider two examples: a one degree-of-freedom system experiencing three sea states operating in a plane to verify the methodology and a 3 degree of freedom system experiencing all six degrees of ship motion to illustrate the ease of computation and complexity of the solution. The first series of simulations explore the impact wave motion has on tracking performance of a position controlled robot. We provide a preliminary comparison between conventional linear control and Repetitive Learning Control (RLC) and show how fixed time delay RLC breaks down due to the varying nature wave disturbance frequency. Next, we explore the impact wave motion disturbances have on Human Amplification Technology (HAT). We begin with a description of the traditional HAT control methodology. Simulations show that the motion of the base of the robot, due to ship motion, generates disturbances forces reflected to the operator that significantly degrade the positioning accuracy and resolution at higher sea states. As with position-controlled manipulators, augmenting the control with a Repetitive

  1. Unicellular eukaryotes as models in cell and molecular biology: critical appraisal of their past and future value.

    Science.gov (United States)

    Simon, Martin; Plattner, Helmut

    2014-01-01

    Unicellular eukaryotes have been appreciated as model systems for the analysis of crucial questions in cell and molecular biology. This includes Dictyostelium (chemotaxis, amoeboid movement, phagocytosis), Tetrahymena (telomere structure, telomerase function), Paramecium (variant surface antigens, exocytosis, phagocytosis cycle) or both ciliates (ciliary beat regulation, surface pattern formation), Chlamydomonas (flagellar biogenesis and beat), and yeast (S. cerevisiae) for innumerable aspects. Nowadays many problems may be tackled with "higher" eukaryotic/metazoan cells for which full genomic information as well as domain databases, etc., were available long before protozoa. Established molecular tools, commercial antibodies, and established pharmacology are additional advantages available for higher eukaryotic cells. Moreover, an increasing number of inherited genetic disturbances in humans have become elucidated and can serve as new models. Among lower eukaryotes, yeast will remain a standard model because of its peculiarities, including its reduced genome and availability in the haploid form. But do protists still have a future as models? This touches not only the basic understanding of biology but also practical aspects of research, such as fund raising. As we try to scrutinize, due to specific advantages some protozoa should and will remain favorable models for analyzing novel genes or specific aspects of cell structure and function. Outstanding examples are epigenetic phenomena-a field of rising interest. © 2014 Elsevier Inc. All rights reserved.

  2. Bactericidal Activity of Ceragenin CSA-13 in Cell Culture and in an Animal Model of Peritoneal Infection.

    Science.gov (United States)

    Bucki, Robert; Niemirowicz, Katarzyna; Wnorowska, Urszula; Byfield, Fitzroy J; Piktel, Ewelina; Wątek, Marzena; Janmey, Paul A; Savage, Paul B

    2015-10-01

    Ceragenins constitute a novel family of cationic antibiotics characterized by a broad spectrum of antimicrobial activities, which have mostly been assessed in vitro. Using a polarized human lung epithelial cell culture system, we evaluated the antibacterial activities of the ceragenin CSA-13 against two strains of Pseudomonas aeruginosa (PAO1 and Xen5). Additionally, the biodistribution and bactericidal activity of a CSA-13-IRDye 800CW derivate were assessed using an animal model of peritoneal infection after PAO1 challenge. In cell culture, CSA-13 bactericidal activities against PAO1 and Xen5 were higher than the activities of the human cathelicidin peptide LL-37. Increased CSA-13 activity was observed in polarized human lung epithelial cell cultures subjected to butyric acid treatment, which is known to increase endogenous LL-37 production. Eight hours after intravenous or intraperitoneal injection, the greatest CSA-13-IRDye 800CW accumulation was observed in mouse liver and kidneys. CSA-13-IRDye 800CW administration resulted in decreased bacterial outgrowth from abdominal fluid collected from animals subjected to intraperitoneal PAO1 infection. These observations indicate that CSA-13 may synergistically interact with antibacterial factors that are naturally present at mucosal surfaces and it maintains its antibacterial activity in the infected abdominal cavity. Cationic lipids such as CSA-13 represent excellent candidates for the development of new antibacterial compounds. Copyright © 2015, American Society for Microbiology. All Rights Reserved.

  3. Current-Sheet Formation and Reconnection at a Magnetic X Line in Particle-in-Cell Simulations

    Science.gov (United States)

    Black, C.; Antiochos, S. K.; Hesse, M.; Karpen, J. T.; Kuznetsova, M. M.; Zenitani, S.

    2011-01-01

    The integration of kinetic effects into macroscopic numerical models is currently of great interest to the heliophysics community, particularly in the context of magnetic reconnection. Reconnection governs the large-scale energy release and topological rearrangement of magnetic fields in a wide variety of laboratory, heliophysical, and astrophysical systems. We are examining the formation and reconnection of current sheets in a simple, two-dimensional X-line configuration using high-resolution particle-in-cell (PIC) simulations. The initial minimum-energy, potential magnetic field is perturbed by excess thermal pressure introduced into the particle distribution function far from the X line. Subsequently, the relaxation of this added stress leads self-consistently to the development of a current sheet that reconnects for imposed stress of sufficient strength. We compare the time-dependent evolution and final state of our PIC simulations with macroscopic magnetohydrodynamic simulations assuming both uniform and localized electrical resistivities (C. R. DeVore et al., this meeting), as well as with force-free magnetic-field equilibria in which the amount of reconnection across the X line can be constrained to be zero (ideal evolution) or optimal (minimum final magnetic energy). We will discuss implications of our results for understanding magnetic-reconnection onset and cessation at kinetic scales in dynamically formed current sheets, such as those occurring in the solar corona and terrestrial magnetotail.

  4. Embedded Systems

    Indian Academy of Sciences (India)

    Embedded system, micro-con- troller ... Embedded systems differ from general purpose computers in many ... Low cost: As embedded systems are extensively used in con- .... operating systems for the desktop computers where scheduling.

  5. Thermal systems; Systemes thermiques

    Energy Technology Data Exchange (ETDEWEB)

    Lalot, S. [Valenciennes Univ. et du Hainaut Cambresis, LME, 59 (France); Lecoeuche, S. [Ecole des Mines de Douai, Dept. GIP, 59 - Douai (France)]|[Lille Univ. des Sciences et Technologies, 59 - Villeneuve d' Ascq (France); Ahmad, M.; Sallee, H.; Quenard, D. [CSTB, 38 - Saint Martin d' Heres (France); Bontemps, A. [Universite Joseph Fourier, LEGI/GRETh, 38 - Grenoble (France); Gascoin, N.; Gillard, P.; Bernard, S. [Laboratoire d' Energetique, Explosion, Structure, 18 - Bourges (France); Gascoin, N.; Toure, Y. [Laboratoire Vision et Robotique, 18 - Bourges (France); Daniau, E.; Bouchez, M. [MBDA, 18 - Bourges (France); Dobrovicescu, A.; Stanciu, D. [Bucarest Univ. Polytechnique, Faculte de Genie Mecanique (Romania); Stoian, M. [Reims Univ. Champagne Ardenne, Faculte des Sciences, UTAP/LTM, 51 (France); Bruch, A.; Fourmigue, J.F.; Colasson, S. [CEA Grenoble, Lab. Greth, 38 (France); Bontemps, A. [Universite Joseph Fourier, LEGI/GRETh, 38 - Grenoble (France); Voicu, I.; Mare, T.; Miriel, J. [Institut National des Sciences Appliquees (INSA), LGCGM, IUT, 35 - Rennes (France); Galanis, N. [Sherbrooke Univ., Genie Mecanique, QC (Canada); Nemer, M.; Clodic, D. [Ecole des Mines de Paris, Centre Energetique et Procedes, 75 (France); Lasbet, Y.; Auvity, B.; Castelain, C.; Peerhossaini, H. [Nantes Univ., Ecole Polytechnique, Lab. de Thermocinetiquede Nantes, UMR-CNRS 6607, 44 (France)

    2005-07-01

    This session about thermal systems gathers 26 articles dealing with: neural model of a compact heat exchanger; experimental study and numerical simulation of the thermal behaviour of test-cells with walls made of a combination of phase change materials and super-insulating materials; hydraulic and thermal modeling of a supercritical fluid with pyrolysis inside a heated channel: pre-dimensioning of an experimental study; energy analysis of the heat recovery devices of a cryogenic system; numerical simulation of the thermo-hydraulic behaviour of a supercritical CO{sub 2} flow inside a vertical tube; mixed convection inside dual-tube exchangers; development of a nodal approach with homogenization for the simulation of the brazing cycle of a heat exchanger; chaotic exchanger for the cooling of low temperature fuel cells; structural optimization of the internal fins of a cylindrical generator; a new experimental approach for the study of the local boiling inside the channels of exchangers with plates and fins; experimental study of the flow regimes of boiling hydrocarbons on a bundle of staggered tubes; energy study of heat recovery exchangers used in Claude-type refrigerating systems; general model of Carnot engine submitted to various operating constraints; the free pistons Stirling cogeneration system; natural gas supplied cogeneration system with polymer membrane fuel cell; influence of the CRN coating on the heat flux inside the tool during the wood unrolling process; transport and mixture of a passive scalar injected inside the wake of a Ahmed body; control of a laser welding-brazing process by infrared thermography; 2D self-adaptative method for contours detection: application to the images of an aniso-thermal jet; exergy and exergy-economical study of an 'Ericsson' engine-based micro-cogeneration system; simplified air-conditioning of telephone switching equipments; parametric study of the 'low-energy' individual dwelling; brief synthesis of

  6. Phosphorylation of protein kinase A (PKA) regulatory subunit RIα by protein kinase G (PKG) primes PKA for catalytic activity in cells.

    Science.gov (United States)

    Haushalter, Kristofer J; Casteel, Darren E; Raffeiner, Andrea; Stefan, Eduard; Patel, Hemal H; Taylor, Susan S

    2018-03-23

    cAMP-dependent protein kinase (PKAc) is a pivotal signaling protein in eukaryotic cells. PKAc has two well-characterized regulatory subunit proteins, RI and RII (each having α and β isoforms), which keep the PKAc catalytic subunit in a catalytically inactive state until activation by cAMP. Previous reports showed that the RIα regulatory subunit is phosphorylated by cGMP-dependent protein kinase (PKG) in vitro , whereupon phosphorylated RIα no longer inhibits PKAc at normal (1:1) stoichiometric ratios. However, the significance of this phosphorylation as a mechanism for activating type I PKA holoenzymes has not been fully explored, especially in cellular systems. In this study, we further examined the potential of RIα phosphorylation to regulate physiologically relevant "desensitization" of PKAc activity. First, the serine 101 site of RIα was validated as a target of PKGIα phosphorylation both in vitro and in cells. Analysis of a phosphomimetic substitution in RIα (S101E) showed that modification of this site increases PKAc activity in vitro and in cells, even without cAMP stimulation. Numerous techniques were used to show that although Ser 101 variants of RIα can bind PKAc, the modified linker region of the S101E mutant has a significantly reduced affinity for the PKAc active site. These findings suggest that RIα phosphorylation may be a novel mechanism to circumvent the requirement of cAMP stimulus to activate type I PKA in cells. We have thus proposed a model to explain how PKG phosphorylation of RIα creates a "sensitized intermediate" state that is in effect primed to trigger PKAc activity.

  7. Gibberellin biosynthesis in cell-free extracts from developing Cucurbita maxima embryos and the identification of new endogenous gibberellins.

    Science.gov (United States)

    Lange, T; Hedden, P; Graebe, J E

    1993-03-01

    Gibberellin (GA) biosynthetic pathways from GA12-aldehyde, GA12 and GA53 were investigated in cell-free systems from developing embryos of Cucurbita maxima L. Gibberellin A12-aldehyde and GA12 were converted to GA25, putative 12α-hydroxyGA25, GA13 and GA39 as main products. Minor products were GA4, GA34 and, when GA12 was the substrate, putative 12α-hydroxyGA12. The intermediates GA15 and GA24 accumulated at low protein concentrations. The influence of various factors on GA12 metabolism was examined. At low 2-oxoglutarate and ascorbate concentrations, or at acid pH, 3β-hydroxylated products predominated, whereas with increasing 2-oxoglutarate and ascorbate concentrations, or at neutral pH, the yield of 12α-hydroxylated GAs increased. Gibberellin A53 was metabolised mainly to the C20-GAs GA44, GA19, GA17, GA23 and GA28, with the C19-GAs GA20, GA1 and GA8 as minor products. Only C19-GAs were 2β-hydroxylated, which is a main characteristic of the embryo systems. In addition to GA13, GA25, GA39, GA43, GA49, GA58, GA74, 12α-hydroxyGA25 and GA39 3-isovalerate, which were known previously from embryos of C. maxima, GA1, GA4, GA17, GA28, GA37, GA38, GA48, GA85, 12α-hydroxyGA37 and putative 12α-hydroxyGA43 were identified as endogenous components by full-scan capillary gas chromatography-mass spectrometry and Kovats retention indices. Evidence for putative 2β-hydroxyGA28 and GA23 was also obtained but it was less conclusive because of contamination.

  8. Data Systems vs. Information Systems

    OpenAIRE

    Amatayakul, Margret K.

    1982-01-01

    This paper examines the current status of “hospital information systems” with respect to the distinction between data systems and information systems. It is proposed that the systems currently existing are incomplete data dystems resulting in ineffective information systems.

  9. Metal plasmon-coupled fluorescence imaging and label free coenzyme detection in cells

    International Nuclear Information System (INIS)

    Zhang, Jian; Fu, Yi; Li, Ge; Zhao, Richard Y.

    2012-01-01

    Highlights: ► Metal nanoparticle for fluorescence cell imaging. ► Non-invasive emission detection of coenzyme in cell on time-resolved confocal microscope. ► Near-field interaction of flavin adenine dinucleotide with silver substrate. ► Isolation of emissions by coenzymes from cellular autofluorescence on fluorescence cell imaging. -- Abstract: Flavin adenine dinucleotide (FAD) is a key metabolite in cellular energy conversion. Flavin can also bind with some enzymes in the metabolic pathway and the binding sites may be changed due to the disease progression. Thus, there is interest on studying its expression level, distribution, and redox state within the cells. FAD is naturally fluorescent, but it has a modest extinction coefficient and quantum yield. Hence the intrinsic emission from FAD is generally too weak to be isolated distinctly from the cellular backgrounds in fluorescence cell imaging. In this article, the metal nanostructures on the glass coverslips were used as substrates to measure FAD in cells. Particulate silver films were fabricated with an optical resonance near the absorption and the emission wavelengths of FAD which can lead to efficient coupling interactions. As a result, the emission intensity and quantum yield by FAD were greatly increased and the lifetime was dramatically shortened resulting in less interference from the longer lived cellular background. This feature may overcome the technical limits that hinder the direct observation of intrinsically fluorescent coenzymes in the cells by fluorescence microscopy. Fluorescence cell imaging on the metallic particle substrates may provide a non-invasive strategy for collecting the information of coenzymes in cells.

  10. Effects of achilline on lipid metabolism gene expression in cell culture

    Directory of Open Access Journals (Sweden)

    A. V. Ratkin

    2016-01-01

    Full Text Available Objective. Evaluation in vitro of the mechanisms of the hypolipidemic effect of sesquiterpene γ-lactone achilline in the hepatoma tissue culture (HTC.Materials and methods.The influence of sesquiterpene γ-lactone achilline and gemfibrozil (comparison drug on the viability, lipid content and expression of key genes of lipid metabolism in the hepatoma tissue culture. The lipid content was assessed by fluorescent method with the vital dye Nile Red, the cell viability was assessed using MTT assay.Results. Cultivation of of cell cultures of rat’s hepatoma cell line HTC for 48 h with achilline in a concentration of from 0.25 to 1.0 mm and gemfibrozil from 0,25 to 0,5 mm did not change cell viability compared to control. In these same concentrations of the test substance reduced the lipid content in the cells, assessed by fluorescent method with the vital dye Nile Red. To study the mechanism of hypolipidemicaction of achillinedetermined the expression of key genes of lipid metabolism in cell culture lines HTC. The possible mechanism of hypolipidemic action of achilline can be attributed to the increased transport and oxidation of long-chain fatty acids in mitochondria, as evidenced by the increase in the gene expression of carnitine-palmitoyltransferase 2 (Cpt2. The decrease in cholesterol level may be due to increased synthesis of bile acids from cholesterol, due to increased gene expression of 7-alphahydroxylase (Cyp7a1. Conclusion. In cell cultures of rat’s hepatoma cell line HTC sesquiterpene γ-lactone achilline reduces the accumulation of lipids in cells, as evidenced by the decrease in the fluorescence of Nile Red, increased gene expression of the carnitine-palmitoyltransferase 2 (Cpt2 gene and 7-alpha-hydroxylase (Cyp7a1.

  11. Metal plasmon-coupled fluorescence imaging and label free coenzyme detection in cells

    Energy Technology Data Exchange (ETDEWEB)

    Zhang, Jian, E-mail: jian@cfs.bioment.umaryland.edu [Center for Fluorescence Spectroscopy, University of Maryland School of Medicine, Department of Biochemistry and Molecular Biology, 725 West Lombard Street, Baltimore, MD 21201 (United States); Fu, Yi [Center for Fluorescence Spectroscopy, University of Maryland School of Medicine, Department of Biochemistry and Molecular Biology, 725 West Lombard Street, Baltimore, MD 21201 (United States); Li, Ge [Division of Molecular Pathology, Department of Pathology, University of Maryland School of Medicine, 10 South Pine Street, Baltimore, MD 21201 (United States); Zhao, Richard Y. [Division of Molecular Pathology, Department of Pathology, University of Maryland School of Medicine, 10 South Pine Street, Baltimore, MD 21201 (United States); Department of Microbiology-Immunology, University of Maryland School of Medicine, 10 South Pine Street, Baltimore, MD 21201 (United States); Institute of Human Virology, University of Maryland School of Medicine, 10 South Pine Street, Baltimore, MD 21201 (United States)

    2012-08-31

    Highlights: Black-Right-Pointing-Pointer Metal nanoparticle for fluorescence cell imaging. Black-Right-Pointing-Pointer Non-invasive emission detection of coenzyme in cell on time-resolved confocal microscope. Black-Right-Pointing-Pointer Near-field interaction of flavin adenine dinucleotide with silver substrate. Black-Right-Pointing-Pointer Isolation of emissions by coenzymes from cellular autofluorescence on fluorescence cell imaging. -- Abstract: Flavin adenine dinucleotide (FAD) is a key metabolite in cellular energy conversion. Flavin can also bind with some enzymes in the metabolic pathway and the binding sites may be changed due to the disease progression. Thus, there is interest on studying its expression level, distribution, and redox state within the cells. FAD is naturally fluorescent, but it has a modest extinction coefficient and quantum yield. Hence the intrinsic emission from FAD is generally too weak to be isolated distinctly from the cellular backgrounds in fluorescence cell imaging. In this article, the metal nanostructures on the glass coverslips were used as substrates to measure FAD in cells. Particulate silver films were fabricated with an optical resonance near the absorption and the emission wavelengths of FAD which can lead to efficient coupling interactions. As a result, the emission intensity and quantum yield by FAD were greatly increased and the lifetime was dramatically shortened resulting in less interference from the longer lived cellular background. This feature may overcome the technical limits that hinder the direct observation of intrinsically fluorescent coenzymes in the cells by fluorescence microscopy. Fluorescence cell imaging on the metallic particle substrates may provide a non-invasive strategy for collecting the information of coenzymes in cells.

  12. EXPERT SYSTEMS

    OpenAIRE

    Georgiana Marin; Mihai Catalin Andrei

    2011-01-01

    In recent decades IT and computer systems have evolved rapidly in economic informatics field. The goal is to create user friendly information systems that respond promptly and accurately to requests. Informatics systems evolved into decision assisted systems, and such systems are converted, based on gained experience, in expert systems for creative problem solving that an organization is facing. Expert systems are aimed at rebuilding human reasoning on the expertise obtained from experts, sto...

  13. Cryo-STEM-EDX spectroscopy for the characterisation of nanoparticles in cell culture media

    Science.gov (United States)

    Ilett, M.; Bamiduro, F.; Matar, O.; Brown, A.; Brydson, R.; Hondow, N.

    2017-09-01

    We present a study of barium titanate nanoparticles dispersed in cell culture media. Scanning transmission electron microscopy combined with energy dispersive X-ray spectroscopy was undertaken on samples prepared using both conventional drop casting and also plunge freezing and examination under cryogenic conditions. This showed that drying artefacts occurred during conventional sample preparation, whereby some salt components of the cell culture media accumulated around the barium titanate nanoparticles; these were removed using the cryogenic route. Importantly, the formation of a calcium and phosphorus rich coating around the barium titanate nanoparticles was retained under cryo-conditions, highlighting that significant interactions do occur between nanomaterials and biological media.

  14. After the Greeting: Realizing the Potential of Physical Models in Cell Biology.

    Science.gov (United States)

    Paluch, Ewa K

    2015-12-01

    Biophysics is increasingly taking center stage in cell biology as the tools for precise quantifications of cellular behaviors expand. Interdisciplinary approaches, combining quantitative physical modeling with cell biology, are of growing interest to journal editors, funding agencies, and hiring committees. However, despite an ever-increasing emphasis on the importance of interdisciplinary research, the student trained in biology may still be at a loss as to what it actually means. I discuss here some considerations on how to achieve meaningful and high-quality interdisciplinary work. Copyright © 2015 Elsevier Ltd. All rights reserved.

  15. Gel-Based Gelatin Zymography to Examine Matrix Metalloproteinase Activity in Cell Culture.

    Science.gov (United States)

    Chhabra, Aastha; Rani, Vibha

    2018-01-01

    Gelatin zymography, first described by Heussen and Dowdle in the 1980s, is a widely used valuable tool in research and diagnostics. The technique identifies gelatinases by the degradation of their preferential substrate as well as by their molecular weight (kDa). We here describe detailed methodology for the detection of pro- and active- forms of both MMP-2 (gelatinase A) and MMP-9 (gelatinase B) in cells using norepinephrine-stimulated H9c2 cardiomyoblasts as model. An easy to follow step-by-step protocol has been carefully written for reliable results. We also suggest an acceptable method for quantification of gelatin zymograms.

  16. Thrust calculation of electric solar wind sail by particle-in-cell simulation

    Energy Technology Data Exchange (ETDEWEB)

    Hoshi, Kento [Kyoto Univ. (Japan). Dept. of Electrical Engineering; Kojima, Hirotsugu; Yamakawa, Hiroshi [Kyoto Univ. (Japan). Research Inst. for Sustainable Humanosphere; Muranaka, Takanobu [Chukyo Univ., Nagoya (Japan). Dept. of Electrical Engineering

    2016-07-01

    In this study, thrust characteristics of an electric solar wind sail were numerically evaluated using full threedimensional particle-in-cell (PIC) simulation. The thrust obtained from the PIC simulation was lower than the thrust estimations obtained in previous studies. The PIC simulation indicated that ambient electrons strongly shield the electrostatic potential of the tether of the sail, and the strong shield effect causes a greater thrust reduction than has been obtained in previous studies. Additionally, previous expressions of the thrust estimation were modified by using the shielded potential structure derived from the present simulation results. The modified thrust estimation agreed very well with the thrust obtained from the PIC simulation.

  17. Finite element approach to global gyrokinetic particle-in-cell simulations using magnetic coordinate

    International Nuclear Information System (INIS)

    Fivaz, M.; Brunner, S.; Ridder, G. de; Sauter, O.; Tran, T.M.; Vaclavik, J.; Villard, L.; Appert, K.

    1997-08-01

    We present a fully-global linear gyrokinetic simulation code (GYGLES) aimed at describing the instable spectrum of the ion-temperature-gradient modes in toroidal geometry. We formulate the Particle-In-Cell method with finite elements defined in magnetic coordinates, which provides excellent numerical convergence properties. The poloidal mode structure corresponding to k // =0 is extracted without approximation from the equations, which reduces drastically the numerical resolution needed. The code can simulate routinely modes with both very long and very short toroidal wavelengths, can treat realistic (MHD) equilibria of any size and runs on a massively parallel computer. (author) 10 figs., 28 refs

  18. Nonequilibrium Gyrokinetic Fluctuation Theory and Sampling Noise in Gyrokinetic Particle-in-cell Simulations

    International Nuclear Information System (INIS)

    Krommes, John A.

    2007-01-01

    The present state of the theory of fluctuations in gyrokinetic (GK) plasmas and especially its application to sampling noise in GK particle-in-cell (PIC) simulations is reviewed. Topics addressed include the Δf method, the fluctuation-dissipation theorem for both classical and GK many-body plasmas, the Klimontovich formalism, sampling noise in PIC simulations, statistical closure for partial differential equations, the theoretical foundations of spectral balance in the presence of arbitrary noise sources, and the derivation of Kadomtsev-type equations from the general formalism

  19. Comparison of the circadian variation in cell proliferation in normal and neoplastic colonic epithelial cells.

    Science.gov (United States)

    Kennedy, M F; Tutton, P J; Barkla, D H

    1985-09-15

    Circadian variations in cell proliferation in normal tissues have been recognised for many years but comparable phenomena in neoplastic tissues appear not to have been reported. Adenomas and carcinomas were induced in mouse colon by injection of dimethylhydrazine (DMH) and cell proliferation in these tumors was measured stathmokinetically. In normal intestine cell proliferation is fastest at night whereas in both adenomas and carcinomas it was found to be slower at night than in the middle of the day. Chemical sympathectomy was found to abolish the circadian variation in tumor cell proliferation.

  20. Comparative Analysis of Click Chemistry Mediated Activity-Based Protein Profiling in Cell Lysates

    Directory of Open Access Journals (Sweden)

    Yinliang Yang

    2013-10-01

    Full Text Available Activity-based protein profiling uses chemical probes that covalently attach to active enzyme targets. Probes with conventional tags have disadvantages, such as limited cell permeability or steric hindrance around the reactive group. A tandem labeling strategy with click chemistry is now widely used to study enzyme targets in situ and in vivo. Herein, the probes are reacted in live cells, whereas the ensuing detection by click chemistry takes place in cell lysates. We here make a comparison of the efficiency of the activity-based tandem labeling strategy by using Cu(I-catalyzed and strain-promoted click chemistry, different ligands and different lysis conditions.

  1. Postendocytic sorting of constitutively internalized dopamine transporter in cell lines and dopaminergic neurons

    DEFF Research Database (Denmark)

    Eriksen, Jacob; Bjørn-Yoshimoto, Walden Emil; Jørgensen, Trine Nygaard

    2010-01-01

    The dopamine transporter (DAT) mediates reuptake of released dopamine and is the target for psychostimulants, such as cocaine and amphetamine. DAT undergoes marked constitutive endocytosis, but little is known about the fate and sorting of the endocytosed transporter. To study DAT sorting in cells...... lines, we fused the one-transmembrane segment protein Tac to DAT, thereby generating a transporter (TacDAT) with an extracellular antibody epitope suited for trafficking studies. TacDAT was functional and endocytosed constitutively in HEK293 cells. According to an ELISA-based assay, TacDAT intracellular...

  2. Particle-in-Cell Code BEAMPATH for Beam Dynamics Simulations in Linear Accelerators and Beamlines

    International Nuclear Information System (INIS)

    Batygin, Y.

    2004-01-01

    A code library BEAMPATH for 2 - dimensional and 3 - dimensional space charge dominated beam dynamics study in linear particle accelerators and beam transport lines is developed. The program is used for particle-in-cell simulation of axial-symmetric, quadrupole-symmetric and z-uniform beams in a channel containing RF gaps, radio-frequency quadrupoles, multipole lenses, solenoids and bending magnets. The programming method includes hierarchical program design using program-independent modules and a flexible combination of modules to provide the most effective version of the structure for every specific case of simulation. Numerical techniques as well as the results of beam dynamics studies are presented

  3. Thrust calculation of electric solar wind sail by particle-in-cell simulation

    International Nuclear Information System (INIS)

    Hoshi, Kento; Kojima, Hirotsugu; Yamakawa, Hiroshi; Muranaka, Takanobu

    2016-01-01

    In this study, thrust characteristics of an electric solar wind sail were numerically evaluated using full threedimensional particle-in-cell (PIC) simulation. The thrust obtained from the PIC simulation was lower than the thrust estimations obtained in previous studies. The PIC simulation indicated that ambient electrons strongly shield the electrostatic potential of the tether of the sail, and the strong shield effect causes a greater thrust reduction than has been obtained in previous studies. Additionally, previous expressions of the thrust estimation were modified by using the shielded potential structure derived from the present simulation results. The modified thrust estimation agreed very well with the thrust obtained from the PIC simulation.

  4. The cytoskeleton in cell-autonomous immunity: structural determinants of host defence

    Science.gov (United States)

    Mostowy, Serge; Shenoy, Avinash R.

    2016-01-01

    Host cells use antimicrobial proteins, pathogen-restrictive compartmentalization and cell death in their defence against intracellular pathogens. Recent work has revealed that four components of the cytoskeleton — actin, microtubules, intermediate filaments and septins, which are well known for their roles in cell division, shape and movement — have important functions in innate immunity and cellular self-defence. Investigations using cellular and animal models have shown that these cytoskeletal proteins are crucial for sensing bacteria and for mobilizing effector mechanisms to eliminate them. In this Review, we highlight the emerging roles of the cytoskeleton as a structural determinant of cell-autonomous host defence. PMID:26292640

  5. Inhibition of Reporter Genes by Small Interfering RNAs in Cell Culture and Living Fish

    DEFF Research Database (Denmark)

    Larashati, Sekar; Schyth, Brian Dall; Lorenzen, Niels

    2011-01-01

    be used to observe the knock down effect by siRNAs designed to target these reporters. One aim of this project is to verify the specific knock down effect of siRNAs in cell culture and in living fish and to establish easy-read out models for testing the effect especially in vivo. Cell culture from human...... coinjection and the assay is important in order to detect knock down by siRNA. Our experiment reveal in vivo knock down at 72 hours post injection of reporter gene and siRNA, but further dose-response experiments are required to confirm specifity....

  6. Particle-in-cell simulations of plasma opening switch with external magnetic field

    International Nuclear Information System (INIS)

    Chen Yulan; Zeng Zhengzhong; Sun Fengju

    2003-01-01

    Fully electromagnetic particle-in-cell simulations are performed to study the effects of an external magnetic field on coaxial plasma opening switch (POS). The simulation results show that POS opening performance can be significantly improved only when external longitudinal magnetic field coils are placed at the cathode side, and an additional azimuthal magnetic field is effective whether the central electrode is of positive or negative polarity. Voltage multiplication coefficient K rises with the additional magnetic field increasing till the electron current is completely magnetically insulated during the opening of POS

  7. Particle-in-Cell Code BEAMPATH for Beam Dynamics Simulations in Linear Accelerators and Beamlines

    Energy Technology Data Exchange (ETDEWEB)

    Batygin, Y.

    2004-10-28

    A code library BEAMPATH for 2 - dimensional and 3 - dimensional space charge dominated beam dynamics study in linear particle accelerators and beam transport lines is developed. The program is used for particle-in-cell simulation of axial-symmetric, quadrupole-symmetric and z-uniform beams in a channel containing RF gaps, radio-frequency quadrupoles, multipole lenses, solenoids and bending magnets. The programming method includes hierarchical program design using program-independent modules and a flexible combination of modules to provide the most effective version of the structure for every specific case of simulation. Numerical techniques as well as the results of beam dynamics studies are presented.

  8. Nonequilibrium Gyrokinetic Fluctuation Theory and Sampling Noise in Gyrokinetic Particle-in-cell Simulations

    Energy Technology Data Exchange (ETDEWEB)

    John A. Krommes

    2007-10-09

    The present state of the theory of fluctuations in gyrokinetic GK plasmas and especially its application to sampling noise in GK particle-in-cell PIC simulations is reviewed. Topics addressed include the Δf method, the fluctuation-dissipation theorem for both classical and GK many-body plasmas, the Klimontovich formalism, sampling noise in PIC simulations, statistical closure for partial differential equations, the theoretical foundations of spectral balance in the presence of arbitrary noise sources, and the derivation of Kadomtsev-type equations from the general formalism.

  9. Productive homologous and non-homologous recombination of hepatitis C virus in cell culture

    DEFF Research Database (Denmark)

    Scheel, Troels K H; Galli, Andrea; Li, Yi-Ping

    2013-01-01

    . In addition, recombination is an important regulatory mechanism of cytopathogenicity for the related pestiviruses. Here we describe recombination of HCV RNA in cell culture leading to production of infectious virus. Initially, hepatoma cells were co-transfected with a replicating JFH1ΔE1E2 genome (genotype 2a......) lacking functional envelope genes and strain J6 (2a), which has functional envelope genes but does not replicate in culture. After an initial decrease in the number of HCV positive cells, infection spread after 13-36 days. Sequencing of recovered viruses revealed non-homologous recombinants with J6...

  10. A simple formalism for diffusion coefficient calculations in cells having a small optical thickness

    International Nuclear Information System (INIS)

    Benoist, Pierre.

    1980-04-01

    A very simple formalism, using directionnal first flight collision probabilities, is established; it is assigned to the calculation of the diffusion coefficients in cells having a small optical thickness. This formalism can be used, at least as a first approximation, in lattices of sodium-cooled fast reactors or of light water reactors. However, due to the two assumptions -cylindricalization of the cell and restriction to the zeroth order term in B 2 (k)- this formalissm cannot be used for sodium-voided or gas-cooled fast reactor lattices [fr

  11. A radiolabel-release microwell assay for proteolytic enzymes present in cell culture media

    International Nuclear Information System (INIS)

    Rucklidge, G.J.; Milne, G.

    1990-01-01

    A modified method for the measurement of proteolytic enzyme activity in cell culture-conditioned media has been developed. Using the release of 3H-labeled peptides from 3H-labeled gelatin the method is performed in microwell plates. The substrate is insolubilized and attached to the wells by glutaraldehyde treatment, thus eliminating the need for a precipitation step at the end of the assay. The assay is sensitive, reproducible, and convenient for small sample volumes. The effect of different protease inhibitors on activity can be assessed rapidly allowing an early characterization of the enzyme. It can also be adapted to microplate spectrophotometric analysis by staining residual substrate with Coomassie blue

  12. Particle-in-cell simulations on spontaneous thermal magnetic field fluctuations

    Energy Technology Data Exchange (ETDEWEB)

    Simões, F. J. R. Jr.; Pavan, J. [Instituto de Física e Matemática, UFPel, Pelotas, RS (Brazil); Gaelzer, R.; Ziebell, L. F. [Instituto de Física, UFRGS, Porto Alegre, RS (Brazil); Yoon, P. H. [Institute for Physical Science and Technology, University of Maryland, College Park, Maryland 20742 (United States)

    2013-10-15

    In this paper an electromagnetic particle code is used to investigate the spontaneous thermal emission. Specifically we perform particle-in-cell simulations employing a non-relativistic isotropic Maxwellian particle distribution to show that thermal fluctuations are related to the origin of spontaneous magnetic field fluctuation. These thermal fluctuations can become seed for further amplification mechanisms and thus be considered at the origin of the cosmological magnetic field, at microgauss levels. Our numerical results are in accordance with theoretical results presented in the literature.

  13. Parallel treatment of simulation particles in particle-in-cell codes on SUPRENUM

    International Nuclear Information System (INIS)

    Seldner, D.

    1990-02-01

    This report contains the program documentation and description of the program package 2D-PLAS, which has been developed at the Nuclear Research Center Karlsruhe in the Institute for Data Processing in Technology (IDT) under the auspices of the BMFT. 2D-PLAS is a parallel program version of the treatment of the simulation particles of the two-dimensional stationary particle-in-cell code BFCPIC which has been developed at the Nuclear Research Center Karlsruhe. This parallel version has been designed for the parallel computer SUPRENUM. (orig.) [de

  14. Effects of chemical and physical agents on recombination events in cells of the germ line of male and female Drosophila melanogaster.

    Science.gov (United States)

    Würgler, F E

    1991-01-01

    Genotoxic agents can induce mutations as well as recombination in the genetic material. The fruit fly Drosophila melanogaster was one of the first assay systems to test physical and chemical agents for recombinogenic effects. Such effects can be observed in cells of the germ line as well as in somatic cells. At present information is available on 54 agents, among them 48 chemicals that have been tested in cells of the germ line of males and/or females. Effects on meiotic recombination in female germ cells cannot simply be classified as positive or negative since for a number of agents, depending on the chromosome region studied, recombination frequencies may be increased, unaffected or decreased. The male germ line of D. melanogaster represents a unique situation because meiotic recombination does not occur. Among 25 agents tested in male germ cells 24 did induce male recombination, among them alkylating, intercalating and cross-linking agents, direct-acting ones as well as compounds needing metabolic activation. With several compounds the frequency of induced recombination is highest in the heterochromatic regions near the centromeres. In brood pattern analyses, e.g., after exposure of adult males to ionizing radiation, the first appearance of crossover progeny is indicative of the sampling of exposed spermatocytes. In premeiotic cells of the male and the female germ line mitotic recombination can occur. Upon clonal expansion of the recombinant cells, clusters of identical crossovers can be observed.

  15. Comparison of α-glucosyl hesperidin of citrus fruits and epigallocatechin gallate of green tea on the Loss of Rotavirus Infectivity in Cell Culture.

    Science.gov (United States)

    Lipson, Steven M; Ozen, Fatma S; Louis, Samantha; Karthikeyan, Laina

    2015-01-01

    A number of secondary plant metabolites (e.g., flavonoids) possess antiviral/antimicrobial activity. Most flavonoids, however, are difficult to study, as they are immiscible in water-based systems. The relatively new semisynthetic α-glucosyl hesperitin (GH), and the natural plant product epigallocatechin gallate (EGCG) are unique among most flavonoids, as these flavonoids are highly soluble. The antiviral activity of these plant metabolites were investigated using the rotavirus as a model enteric virus system. Direct loss of virus structural integrity in cell-free suspension and titration of amplified RTV in host cell cultures was measured by a quantitative enzyme-linked immunosorbent assay (qEIA). After 30 min. 100 × 10(3) μg/ml GH reduced RTV antigen levels by ca. 90%. The same compound reduced infectivity (replication in cell culture) by a similar order of magnitude 3 to 4 days post inoculation. After 3 days in culture, EGCG concentrations of 80, 160, and 320 μg/ml reduced RTV infectivity titer levels to ca. 50, 20, and 15% of the control, respectively. Loss of RTV infectivity titers occurred following viral treatment by parallel testing of both GH and EGCG, with the latter, markedly more effective. Cytotoxicity testing showed no adverse effects by the phenolic concentrations used in this study. The unique chemical structure of each flavonoid rather than each phenolic's inherent solubility may be ascribed to those marked differences between each molecule's antiviral (anti-RTV) effects. The solubility of EGCG and GH obviated our need to use potentially confounding or obfuscating carrier molecules (e.g., methanol, ethanol, DMSO) denoting our use of a pure system environ. Our work further denotes the need to address the unique chemical nature of secondary plant metabolites before any broad generalizations in flavonoid (antiviral) activity may be proposed.

  16. PICK1 expression in the Drosophila central nervous system primarily occurs in the neuroendocrine system

    DEFF Research Database (Denmark)

    Jansen, Anna M; Nässel, Dick R; Madsen, Kenneth L

    2009-01-01

    in the adult and larval Drosophila central nervous system. PICK1 was found in cell bodies in the subesophageal ganglion, the antennal lobe, the protocerebrum, and the neuroendocrine center pars intercerebralis. The cell types that express PICK1 were identified using GAL4 enhancer trap lines. The PICK1...... (AMPA) receptor subunit GluR2 and the dopamine transporter. PICK1 is strongly implicated in GluR2 trafficking and synaptic plasticity. In mammals, PICK1 has been characterized extensively in cell culture studies. To study PICK1 in an intact system, we characterized PICK1 expression immunohistochemically...... neurons in the neuroendocrine system, which express the transcription factor DIMM and the amidating enzyme peptidylglycine-alpha-hydroxylating monooxygenase (PHM). The PICK1-positive cells include neurosecretory cells that produce the insulin-like peptide dILP2. PICK1 expression in insulin-producing cells...

  17. Multibody Systems

    DEFF Research Database (Denmark)

    Wagner, Falko Jens

    1999-01-01

    Multibody Systems is one area, in which methods for solving DAEs are of special interst. This chapter is about multibody systems, why they result in DAE systems and what kind of problems that can arise when dealing with multibody systems and formulating their corresponding DAE system....

  18. A three-dimensional electrostatic particle-in-cell methodology on unstructured Delaunay-Voronoi grids

    International Nuclear Information System (INIS)

    Gatsonis, Nikolaos A.; Spirkin, Anton

    2009-01-01

    The mathematical formulation and computational implementation of a three-dimensional particle-in-cell methodology on unstructured Delaunay-Voronoi tetrahedral grids is presented. The method allows simulation of plasmas in complex domains and incorporates the duality of the Delaunay-Voronoi in all aspects of the particle-in-cell cycle. Charge assignment and field interpolation weighting schemes of zero- and first-order are formulated based on the theory of long-range constraints. Electric potential and fields are derived from a finite-volume formulation of Gauss' law using the Voronoi-Delaunay dual. Boundary conditions and the algorithms for injection, particle loading, particle motion, and particle tracking are implemented for unstructured Delaunay grids. Error and sensitivity analysis examines the effects of particles/cell, grid scaling, and timestep on the numerical heating, the slowing-down time, and the deflection times. The problem of current collection by cylindrical Langmuir probes in collisionless plasmas is used for validation. Numerical results compare favorably with previous numerical and analytical solutions for a wide range of probe radius to Debye length ratios, probe potentials, and electron to ion temperature ratios. The versatility of the methodology is demonstrated with the simulation of a complex plasma microsensor, a directional micro-retarding potential analyzer that includes a low transparency micro-grid.

  19. Enhanced stopping of macro-particles in particle-in-cell simulations

    International Nuclear Information System (INIS)

    May, J.; Tonge, J.; Ellis, I.; Mori, W. B.; Fiuza, F.; Fonseca, R. A.; Silva, L. O.; Ren, C.

    2014-01-01

    We derive an equation for energy transfer from relativistic charged particles to a cold background plasma appropriate for finite-size particles that are used in particle-in-cell simulation codes. Expressions for one-, two-, and three-dimensional particles are presented, with special attention given to the two-dimensional case. This energy transfer is due to the electric field of the wake set up in the background plasma by the relativistic particle. The enhanced stopping is dependent on the q 2 /m, where q is the charge and m is the mass of the relativistic particle, and therefore simulation macro-particles with large charge but identical q/m will stop more rapidly. The stopping power also depends on the effective particle shape of the macro-particle. These conclusions are verified in particle-in-cell simulations. We present 2D simulations of test particles, relaxation of high-energy tails, and integrated fast ignition simulations showing that the enhanced drag on macro-particles may adversely affect the results of these simulations in a wide range of high-energy density plasma scenarios. We also describe a particle splitting algorithm which can potentially overcome this problem and show its effect in controlling the stopping of macro-particles

  20. RP1 is a phosphorylation target of CK2 and is involved in cell adhesion.

    Science.gov (United States)

    Stenner, Frank; Liewen, Heike; Göttig, Stephan; Henschler, Reinhard; Markuly, Norbert; Kleber, Sascha; Faust, Michael; Mischo, Axel; Bauer, Stefan; Zweifel, Martin; Knuth, Alexander; Renner, Christoph; Wadle, Andreas

    2013-01-01

    RP1 (synonym: MAPRE2, EB2) is a member of the microtubule binding EB1 protein family, which interacts with APC, a key regulatory molecule in the Wnt signalling pathway. While the other EB1 proteins are well characterized the cellular function and regulation of RP1 remain speculative to date. However, recently RP1 has been implicated in pancreatic cancerogenesis. CK2 is a pleiotropic kinase involved in adhesion, proliferation and anti-apoptosis. Overexpression of protein kinase CK2 is a hallmark of many cancers and supports the malignant phenotype of tumor cells. In this study we investigate the interaction of protein kinase CK2 with RP1 and demonstrate that CK2 phosphorylates RP1 at Ser(236) in vitro. Stable RP1 expression in cell lines leads to a significant cleavage and down-regulation of N-cadherin and impaired adhesion. Cells expressing a Phospho-mimicking point mutant RP1-ASP(236) show a marked decrease of adhesion to endothelial cells under shear stress. Inversely, we found that the cells under shear stress downregulate endogenous RP1, most likely to improve cellular adhesion. Accordingly, when RP1 expression is suppressed by shRNA, cells lacking RP1 display significantly increased cell adherence to surfaces. In summary, RP1 phosphorylation at Ser(236) by CK2 seems to play a significant role in cell adhesion and might initiate new insights in the CK2 and EB1 family protein association.

  1. RP1 is a phosphorylation target of CK2 and is involved in cell adhesion.

    Directory of Open Access Journals (Sweden)

    Frank Stenner

    Full Text Available RP1 (synonym: MAPRE2, EB2 is a member of the microtubule binding EB1 protein family, which interacts with APC, a key regulatory molecule in the Wnt signalling pathway. While the other EB1 proteins are well characterized the cellular function and regulation of RP1 remain speculative to date. However, recently RP1 has been implicated in pancreatic cancerogenesis. CK2 is a pleiotropic kinase involved in adhesion, proliferation and anti-apoptosis. Overexpression of protein kinase CK2 is a hallmark of many cancers and supports the malignant phenotype of tumor cells. In this study we investigate the interaction of protein kinase CK2 with RP1 and demonstrate that CK2 phosphorylates RP1 at Ser(236 in vitro. Stable RP1 expression in cell lines leads to a significant cleavage and down-regulation of N-cadherin and impaired adhesion. Cells expressing a Phospho-mimicking point mutant RP1-ASP(236 show a marked decrease of adhesion to endothelial cells under shear stress. Inversely, we found that the cells under shear stress downregulate endogenous RP1, most likely to improve cellular adhesion. Accordingly, when RP1 expression is suppressed by shRNA, cells lacking RP1 display significantly increased cell adherence to surfaces. In summary, RP1 phosphorylation at Ser(236 by CK2 seems to play a significant role in cell adhesion and might initiate new insights in the CK2 and EB1 family protein association.

  2. Microvesicle and tunneling nanotube mediated intercellular transfer of g-protein coupled receptors in cell cultures

    International Nuclear Information System (INIS)

    Guescini, M.; Leo, G.; Genedani, S.; Carone, C.; Pederzoli, F.; Ciruela, F.; Guidolin, D.; Stocchi, V.; Mantuano, M.; Borroto-Escuela, D.O.; Fuxe, K.; Agnati, L.F.

    2012-01-01

    Recent evidence shows that cells exchange collections of signals via microvesicles (MVs) and tunneling nano-tubes (TNTs). In this paper we have investigated whether in cell cultures GPCRs can be transferred by means of MVs and TNTs from a source cell to target cells. Western blot, transmission electron microscopy and gene expression analyses demonstrate that A 2A and D 2 receptors are present in released MVs. In order to further demonstrate the involvement of MVs in cell-to-cell communication we created two populations of cells (HEK293T and COS-7) transiently transfected with D 2 R-CFP or A 2A R-YFP. These two types of cells were co-cultured, and FRET analysis demonstrated simultaneously positive cells to the D 2 R-CFP and A 2A R-YFP. Fluorescence microscopy analysis also showed that GPCRs can move from one cell to another also by means of TNTs. Finally, recipient cells pre-incubated for 24 h with A 2A R positive MVs were treated with the adenosine A 2A receptor agonist CGS-21680. The significant increase in cAMP accumulation clearly demonstrated that A 2A Rs were functionally competent in target cells. These findings demonstrate that A 2A receptors capable of recognizing and decoding extracellular signals can be safely transferred via MVs from source to target cells.

  3. Cell in situ zymography: an in vitro cytotechnology for localization of enzyme activity in cell culture.

    Science.gov (United States)

    Chhabra, Aastha; Jaiswal, Astha; Malhotra, Umang; Kohli, Shrey; Rani, Vibha

    2012-09-01

    In situ zymography is a unique technique for detection and localization of enzyme-substrate interactions majorly in histological sections. Substrate with quenched fluorogenic molecule is incorporated in gel over which tissue sections are mounted and then incubated in buffer. The enzymatic activity is observed in the form of fluorescent signal. With the advancements in the field of biological research, use of in vitro cell culture has become very popular and holds great significance in multiple fields including inflammation, cancer, stem cell biology and the still emerging 3-D cell cultures. The information on analysis of enzymatic activity in cell lines is inadequate presently. We propose a single-step methodology that is simple, sensitive, cost-effective, and functional to perform and study the 'in position' activity of enzyme on substrate for in vitro cell cultures. Quantification of enzymatic activity to carry out comparative studies on cells has also been illustrated. This technique can be applied to a variety of enzyme classes including proteases, amylases, xylanases, and cellulases in cell cultures.

  4. Functional enhancement of chitosan and nanoparticles in cell culture, tissue engineering, and pharmaceutical applications

    Directory of Open Access Journals (Sweden)

    Wenjuan eGao

    2012-08-01

    Full Text Available Abstract: As a biomaterial, chitosan has been widely used in tissue engineering, wound healing, drug delivery, and other biomedical applications. It can be formulated in a variety of forms, such as powder, film, sphere, gel and fiber. These features make chitosan an almost ideal biomaterial in cell culture applications, and cell cultures arguably constitute the most practical way to evaluate biocompatibility and biotoxicity. The advantages of cell cultures are that they can be performed under totally controlled environments, allow high throughput functional screening, and are less costly, as compared to other assessment methods. Chitosan can also be modified into multilayer composite by combining with other polymers and moieties to alter the properties of chitosan for particular biomedical applications. This review briefly depicts and discusses applications of chitosan and nanoparticles in cell culture, in particular, the effects of chitosan and nanoparticles on cell adhesion, cell survival, and the underlying molecular mechanisms: both stimulatory and inhibitory influences are discussed. Our aim is to update the current status of how nanoparticles can be utilized to modify the properties of chitosan to advance the art of tissue engineering by using cell cultures.

  5. Agglomeration, sedimentation, and cellular toxicity of alumina nanoparticles in cell culture medium

    International Nuclear Information System (INIS)

    Yoon, Dokyung; Woo, Daekwang; Kim, Jung Heon; Kim, Moon Ki; Kim, Taesung; Hwang, Eung-Soo; Baik, Seunghyun

    2011-01-01

    The cytotoxicity of alumina nanoparticles (NPs) was investigated for a wide range of concentration (25–200 μg/mL) and incubation time (0–72 h) using floating cells (THP-1) and adherent cells (J774A.1, A549, and 293). Alumina NPs were gradually agglomerated over time although a significant portion of sedimentation occurred at the early stage within 6 h. A decrease of the viability was found in floating (THP-1) and adherent (J774A.1 and A549) cells in a dose-dependent manner. However, the time-dependent decrease in cell viability was observed only in adherent cells (J774A.1 and A549), which is predominantly related with the sedimentation of alumina NPs in cell culture medium. The uptake of alumina NPs in macrophages and an increased cell-to-cell adhesion in adherent cells were observed. There was no significant change in the viability of 293 cells. This in vitro test suggests that the agglomeration and sedimentation of alumina NPs affected cellular viability depending on cell types such as monocytes (THP-1), macrophages (J774A.1), lung carcinoma cells (A549), and embryonic kidney cells (293).

  6. Agglomeration, sedimentation, and cellular toxicity of alumina nanoparticles in cell culture medium

    Science.gov (United States)

    Yoon, Dokyung; Woo, Daekwang; Kim, Jung Heon; Kim, Moon Ki; Kim, Taesung; Hwang, Eung-Soo; Baik, Seunghyun

    2011-06-01

    The cytotoxicity of alumina nanoparticles (NPs) was investigated for a wide range of concentration (25-200 μg/mL) and incubation time (0-72 h) using floating cells (THP-1) and adherent cells (J774A.1, A549, and 293). Alumina NPs were gradually agglomerated over time although a significant portion of sedimentation occurred at the early stage within 6 h. A decrease of the viability was found in floating (THP-1) and adherent (J774A.1 and A549) cells in a dose-dependent manner. However, the time-dependent decrease in cell viability was observed only in adherent cells (J774A.1 and A549), which is predominantly related with the sedimentation of alumina NPs in cell culture medium. The uptake of alumina NPs in macrophages and an increased cell-to-cell adhesion in adherent cells were observed. There was no significant change in the viability of 293 cells. This in vitro test suggests that the agglomeration and sedimentation of alumina NPs affected cellular viability depending on cell types such as monocytes (THP-1), macrophages (J774A.1), lung carcinoma cells (A549), and embryonic kidney cells (293).

  7. Particle-in-Cell laser-plasma simulation on Xeon Phi coprocessors

    Science.gov (United States)

    Surmin, I. A.; Bastrakov, S. I.; Efimenko, E. S.; Gonoskov, A. A.; Korzhimanov, A. V.; Meyerov, I. B.

    2016-05-01

    This paper concerns the development of a high-performance implementation of the Particle-in-Cell method for plasma simulation on Intel Xeon Phi coprocessors. We discuss the suitability of the method for Xeon Phi architecture and present our experience in the porting and optimization of the existing parallel Particle-in-Cell code PICADOR. Direct porting without code modification gives performance on Xeon Phi close to that of an 8-core CPU on a benchmark problem with 50 particles per cell. We demonstrate step-by-step optimization techniques, such as improving data locality, enhancing parallelization efficiency and vectorization leading to an overall 4.2 × speedup on CPU and 7.5 × on Xeon Phi compared to the baseline version. The optimized version achieves 16.9 ns per particle update on an Intel Xeon E5-2660 CPU and 9.3 ns per particle update on an Intel Xeon Phi 5110P. For a real problem of laser ion acceleration in targets with surface grating, where a large number of macroparticles per cell is required, the speedup of Xeon Phi compared to CPU is 1.6 ×.

  8. Load-balancing techniques for a parallel electromagnetic particle-in-cell code

    Energy Technology Data Exchange (ETDEWEB)

    PLIMPTON,STEVEN J.; SEIDEL,DAVID B.; PASIK,MICHAEL F.; COATS,REBECCA S.

    2000-01-01

    QUICKSILVER is a 3-d electromagnetic particle-in-cell simulation code developed and used at Sandia to model relativistic charged particle transport. It models the time-response of electromagnetic fields and low-density-plasmas in a self-consistent manner: the fields push the plasma particles and the plasma current modifies the fields. Through an LDRD project a new parallel version of QUICKSILVER was created to enable large-scale plasma simulations to be run on massively-parallel distributed-memory supercomputers with thousands of processors, such as the Intel Tflops and DEC CPlant machines at Sandia. The new parallel code implements nearly all the features of the original serial QUICKSILVER and can be run on any platform which supports the message-passing interface (MPI) standard as well as on single-processor workstations. This report describes basic strategies useful for parallelizing and load-balancing particle-in-cell codes, outlines the parallel algorithms used in this implementation, and provides a summary of the modifications made to QUICKSILVER. It also highlights a series of benchmark simulations which have been run with the new code that illustrate its performance and parallel efficiency. These calculations have up to a billion grid cells and particles and were run on thousands of processors. This report also serves as a user manual for people wishing to run parallel QUICKSILVER.

  9. Influence of the circadian rhythm in cell division on radiation-induced mitotic delay in vivo

    International Nuclear Information System (INIS)

    Rubin, N.A.

    1980-01-01

    All mitotically active normal tissues in mammals investigated to date demonstrate a circadian rhythm in cell division. The murine corneal epithelium is a practical and advantageous tissue model for studying this phenomenon. In animals synchronized to a light-dark (LD) schedule, one sees predictably reproducible occurrences of peaks and troughs in the mitotic index (MI) within each 24-hour (h) period. One of the harmful effects of ionizing radiation on dividing cells is mitotic delay, reported to be a G 2 block in cells approaching mitosis. Affected cells are not killed but are inhibited from entering mitosis and are delayed for a span of time reported to be dose and cell cycle dependent. In the classical description of mitotic delay, MI of irradiated cells begins to drop in relation to the control, which is plotted as a straight line, uniform throughout the experiment. After the damage is repaired, delayed cells can enter mitosis along with other cells in the pool unaffected by the radiation, resulting in a MI higher than control levels. The span of delay and the occurrence of recovery are assumed to be constant for a given dose and tissue under similar experimental conditions. First described in asynchronously-dividing tissue culture cells, this concept is also extrapolated to the in vivo situation

  10. ASPEN: A fully kinetic, reduced-description particle-in-cell model for simulating parametric instabilities

    International Nuclear Information System (INIS)

    Vu, H.X.; Bezzerides, B.; DuBois, D.F.

    1999-01-01

    A fully kinetic, reduced-description particle-in-cell (RPIC) model is presented in which deviations from quasineutrality, electron and ion kinetic effects, and nonlinear interactions between low-frequency and high-frequency parametric instabilities are modeled correctly. The model is based on a reduced description where the electromagnetic field is represented by three separate temporal envelopes in order to model parametric instabilities with low-frequency and high-frequency daughter waves. Because temporal envelope approximations are invoked, the simulation can be performed on the electron time scale instead of the time scale of the light waves. The electrons and ions are represented by discrete finite-size particles, permitting electron and ion kinetic effects to be modeled properly. The Poisson equation is utilized to ensure that space-charge effects are included. The RPIC model is fully three dimensional and has been implemented in two dimensions on the Accelerated Strategic Computing Initiative (ASCI) parallel computer at Los Alamos National Laboratory, and the resulting simulation code has been named ASPEN. The authors believe this code is the first particle-in-cell code capable of simulating the interaction between low-frequency and high-frequency parametric instabilities in multiple dimensions. Test simulations of stimulated Raman scattering, stimulated Brillouin scattering, and Langmuir decay instability are presented

  11. Failure of lysosome clustering and positioning in the juxtanuclear region in cells deficient in rapsyn

    Science.gov (United States)

    Aittaleb, Mohamed; Chen, Po-Ju; Akaaboune, Mohammed

    2015-01-01

    ABSTRACT Rapsyn, a scaffold protein, is required for the clustering of acetylcholine receptors (AChRs) at contacts between motor neurons and differentiating muscle cells. Rapsyn is also expressed in cells that do not express AChRs. However, its function in these cells remains unknown. Here, we show that rapsyn plays an AChR-independent role in organizing the distribution and mobility of lysosomes. In cells devoid of AChRs, rapsyn selectively induces the clustering of lysosomes at high density in the juxtanuclear region without affecting the distribution of other intracellular organelles. However, when the same cells overexpress AChRs, rapsyn is recruited away from lysosomes to colocalize with AChR clusters on the cell surface. In rapsyn-deficient (Rapsn−/−) myoblasts or cells overexpressing rapsyn mutants, lysosomes are scattered within the cell and highly dynamic. The increased mobility of lysosomes in Rapsn−/− cells is associated with a significant increase in lysosomal exocytosis, as evidenced by increased release of lysosomal enzymes and plasma membrane damage when cells were challenged with the bacterial pore-forming toxin streptolysin-O. These findings uncover a new link between rapsyn, lysosome positioning, exocytosis and plasma membrane integrity. PMID:26330529

  12. photon-plasma: A modern high-order particle-in-cell code

    International Nuclear Information System (INIS)

    Haugbølle, Troels; Frederiksen, Jacob Trier; Nordlund, Åke

    2013-01-01

    We present the photon-plasma code, a modern high order charge conserving particle-in-cell code for simulating relativistic plasmas. The code is using a high order implicit field solver and a novel high order charge conserving interpolation scheme for particle-to-cell interpolation and charge deposition. It includes powerful diagnostics tools with on-the-fly particle tracking, synthetic spectra integration, 2D volume slicing, and a new method to correctly account for radiative cooling in the simulations. A robust technique for imposing (time-dependent) particle and field fluxes on the boundaries is also presented. Using a hybrid OpenMP and MPI approach, the code scales efficiently from 8 to more than 250.000 cores with almost linear weak scaling on a range of architectures. The code is tested with the classical benchmarks particle heating, cold beam instability, and two-stream instability. We also present particle-in-cell simulations of the Kelvin-Helmholtz instability, and new results on radiative collisionless shocks

  13. Enhancement effect of shikonin in cell suspension culture and transfermanant culture by radiation application

    International Nuclear Information System (INIS)

    Kim, Jae Sung; Lee, Young Keun; Chung, Byung Yeoup; Lee, Young Bok; Hwang Hye Yeon

    2004-10-01

    The cell lines 679, 679-29 and 622-46 of L. erythrorhizon could be selected on LS agar medium for the production shikonin in cell suspension culture. The shikonin was increased moderately in suspension culture of cell line 622-46 in LS liquid medium containing BA 2 mg·L -1 and IAA 0.2 mg·L -1 in the dark, and was increased by adding 1 μM Cu 2+ and 100 μM methyl jasmonate The accumulation of shikonin in the liquid medium was increased significantly by 2 Gy irradiation to callus of cell line 622-46 and culture in LS liquid medium containing BA 2 mg·L -1 and IAA 0.2 mg·L -1 in the dark and shikonin in cell debris was higher by 16 Gy irradiation. The activity of p-hydroxybenzoate geranyltransferase was increased by irradiation of 2 Gy and 16 Gy of γ radiation. Seedling hypocotyles of L. erythrorhizon were infected with Agrogacterium rhizogenes strain 15834 harboring a binary vector with an intron bearing the GUS (β-glucuronidase) gene driven by cauliflower mosaic virus (CaMV) 35S promotor as well as the HPT (hygromycin phosphotransferase) gene as the selection marker. Hairy roots isolated were hygromycin resistant and had integrated GUS gene in DNA. The root tip grown on M-9 medium showed normal pigment production pattern in border cells and root hairs

  14. Load-balancing techniques for a parallel electromagnetic particle-in-cell code

    International Nuclear Information System (INIS)

    Plimpton, Steven J.; Seidel, David B.; Pasik, Michael F.; Coats, Rebecca S.

    2000-01-01

    QUICKSILVER is a 3-d electromagnetic particle-in-cell simulation code developed and used at Sandia to model relativistic charged particle transport. It models the time-response of electromagnetic fields and low-density-plasmas in a self-consistent manner: the fields push the plasma particles and the plasma current modifies the fields. Through an LDRD project a new parallel version of QUICKSILVER was created to enable large-scale plasma simulations to be run on massively-parallel distributed-memory supercomputers with thousands of processors, such as the Intel Tflops and DEC CPlant machines at Sandia. The new parallel code implements nearly all the features of the original serial QUICKSILVER and can be run on any platform which supports the message-passing interface (MPI) standard as well as on single-processor workstations. This report describes basic strategies useful for parallelizing and load-balancing particle-in-cell codes, outlines the parallel algorithms used in this implementation, and provides a summary of the modifications made to QUICKSILVER. It also highlights a series of benchmark simulations which have been run with the new code that illustrate its performance and parallel efficiency. These calculations have up to a billion grid cells and particles and were run on thousands of processors. This report also serves as a user manual for people wishing to run parallel QUICKSILVER

  15. Microvesicle and tunneling nanotube mediated intercellular transfer of g-protein coupled receptors in cell cultures

    Energy Technology Data Exchange (ETDEWEB)

    Guescini, M. [Department of Biomolecular Sciences, University of Urbino ' Carlo Bo' , 61029 Urbino (Italy); Leo, G.; Genedani, S. [Department Biomedical Sciences, University of Modena and Reggio Emilia (Italy); Carone, C. [Department Biomedical Sciences, University of Modena and Reggio Emilia (Italy); IRCCS San Camillo Lido, Venezia (Italy); Pederzoli, F. [Department Biomedical Sciences, University of Modena and Reggio Emilia (Italy); Ciruela, F. [Departament Patologia i Terapeutica Experimental, Universitat de Barcelona (Spain); Guidolin, D. [Department of Human Anatomy and Physiology, University of Padua (Italy); Stocchi, V.; Mantuano, M. [Department of Biomolecular Sciences, University of Urbino ' Carlo Bo' , 61029 Urbino (Italy); Borroto-Escuela, D.O.; Fuxe, K. [Department of Neuroscience, Karolinska Institutet, Stockholm (Sweden); Agnati, L.F., E-mail: luigiagnati@tin.it [IRCCS San Camillo Lido, Venezia (Italy)

    2012-03-10

    Recent evidence shows that cells exchange collections of signals via microvesicles (MVs) and tunneling nano-tubes (TNTs). In this paper we have investigated whether in cell cultures GPCRs can be transferred by means of MVs and TNTs from a source cell to target cells. Western blot, transmission electron microscopy and gene expression analyses demonstrate that A{sub 2A} and D{sub 2} receptors are present in released MVs. In order to further demonstrate the involvement of MVs in cell-to-cell communication we created two populations of cells (HEK293T and COS-7) transiently transfected with D{sub 2}R-CFP or A{sub 2A}R-YFP. These two types of cells were co-cultured, and FRET analysis demonstrated simultaneously positive cells to the D{sub 2}R-CFP and A{sub 2A}R-YFP. Fluorescence microscopy analysis also showed that GPCRs can move from one cell to another also by means of TNTs. Finally, recipient cells pre-incubated for 24 h with A{sub 2A}R positive MVs were treated with the adenosine A{sub 2A} receptor agonist CGS-21680. The significant increase in cAMP accumulation clearly demonstrated that A{sub 2A}Rs were functionally competent in target cells. These findings demonstrate that A{sub 2A} receptors capable of recognizing and decoding extracellular signals can be safely transferred via MVs from source to target cells.

  16. Cloud-In-Cell modeling of shocked particle-laden flows at a ``SPARSE'' cost

    Science.gov (United States)

    Taverniers, Soren; Jacobs, Gustaaf; Sen, Oishik; Udaykumar, H. S.

    2017-11-01

    A common tool for enabling process-scale simulations of shocked particle-laden flows is Eulerian-Lagrangian Particle-Source-In-Cell (PSIC) modeling where each particle is traced in its Lagrangian frame and treated as a mathematical point. Its dynamics are governed by Stokes drag corrected for high Reynolds and Mach numbers. The computational burden is often reduced further through a ``Cloud-In-Cell'' (CIC) approach which amalgamates groups of physical particles into computational ``macro-particles''. CIC does not account for subgrid particle fluctuations, leading to erroneous predictions of cloud dynamics. A Subgrid Particle-Averaged Reynolds-Stress Equivalent (SPARSE) model is proposed that incorporates subgrid interphase velocity and temperature perturbations. A bivariate Gaussian source distribution, whose covariance captures the cloud's deformation to first order, accounts for the particles' momentum and energy influence on the carrier gas. SPARSE is validated by conducting tests on the interaction of a particle cloud with the accelerated flow behind a shock. The cloud's average dynamics and its deformation over time predicted with SPARSE converge to their counterparts computed with reference PSIC models as the number of Gaussians is increased from 1 to 16. This work was supported by AFOSR Grant No. FA9550-16-1-0008.

  17. Optimal reliability and upgrading decisions for capital goods

    NARCIS (Netherlands)

    Öner, K.B.

    2010-01-01

    Advanced technical systems, also called advanced capital goods (e.g. medical systems, material handling systems, defense systems, manufacturing systems, packaging lines, computer networks) are used in core processes by their users. By core processes, we mean the processes which are essential for

  18. System dynamics

    International Nuclear Information System (INIS)

    Kim, Do Hun; Mun, Tae Hun; Kim, Dong Hwan

    1999-02-01

    This book introduces systems thinking and conceptual tool and modeling tool of dynamics system such as tragedy of single thinking, accessible way of system dynamics, feedback structure and causal loop diagram analysis, basic of system dynamics modeling, causal loop diagram and system dynamics modeling, information delay modeling, discovery and application for policy, modeling of crisis of agricultural and stock breeding products, dynamic model and lesson in ecosystem, development and decadence of cites and innovation of education forward system thinking.

  19. Multimodal Processes Rescheduling

    DEFF Research Database (Denmark)

    Bocewicz, Grzegorz; Banaszak, Zbigniew A.; Nielsen, Peter

    2013-01-01

    Cyclic scheduling problems concerning multimodal processes are usually observed in FMSs producing multi-type parts where the Automated Guided Vehicles System (AGVS) plays a role of a material handling system. Schedulability analysis of concurrently flowing cyclic processes (SCCP) exe-cuted in the......Cyclic scheduling problems concerning multimodal processes are usually observed in FMSs producing multi-type parts where the Automated Guided Vehicles System (AGVS) plays a role of a material handling system. Schedulability analysis of concurrently flowing cyclic processes (SCCP) exe...

  20. Expression of WNT genes in cervical cancer-derived cells: Implication of WNT7A in cell proliferation and migration

    International Nuclear Information System (INIS)

    Ramos-Solano, Moisés; Meza-Canales, Ivan D.; Torres-Reyes, Luis A.; Alvarez-Zavala, Monserrat

    2015-01-01

    According to the multifactorial model of cervical cancer (CC) causation, it is now recognized that other modifications, in addition to Human papillomavirus (HPV) infection, are necessary for the development of this neoplasia. Among these, it has been proposed that a dysregulation of the WNT pathway might favor malignant progression of HPV-immortalized keratinocytes. The aim of this study was to identify components of the WNT pathway differentially expressed in CC vs. non-tumorigenic, but immortalized human keratinocytes. Interestingly, WNT7A expression was found strongly downregulated in cell lines and biopsies derived from CC. Restoration of WNT7A in CC-derived cell lines using a lentiviral gene delivery system or after adding a recombinant human protein decreases cell proliferation. Likewise, WNT7A silencing in non-tumorigenic cells markedly accelerates proliferation. Decreased WNT7A expression was due to hypermethylation at particular CpG sites. To our knowledge, this is the first study reporting reduced WNT7A levels in CC-derived cells and that ectopic WNT7A restoration negatively affects cell proliferation and migration. - Highlights: • WNT7A is expressed in normal keratinocytes or cervical cells without lesion. • WNT7A is significantly reduced in cervical cancer-derived cells. • Restoration of WNT7A expression in HeLa decreases proliferation and cell migration. • Silencing of WNT7A in HaCaT induces an increased proliferation and migration rate. • Decreased WNT7A expression in this model is due to hypermethylation

  1. Virtual Reconstruction and Three-Dimensional Printing of Blood Cells as a Tool in Cell Biology Education.

    Science.gov (United States)

    Augusto, Ingrid; Monteiro, Douglas; Girard-Dias, Wendell; Dos Santos, Thaisa Oliveira; Rosa Belmonte, Simone Letícia; Pinto de Oliveira, Jairo; Mauad, Helder; da Silva Pacheco, Marcos; Lenz, Dominik; Stefanon Bittencourt, Athelson; Valentim Nogueira, Breno; Lopes Dos Santos, Jorge Roberto; Miranda, Kildare; Guimarães, Marco Cesar Cunegundes

    2016-01-01

    The cell biology discipline constitutes a highly dynamic field whose concepts take a long time to be incorporated into the educational system, especially in developing countries. Amongst the main obstacles to the introduction of new cell biology concepts to students is their general lack of identification with most teaching methods. The introduction of elaborated figures, movies and animations to textbooks has given a tremendous contribution to the learning process and the search for novel teaching methods has been a central goal in cell biology education. Some specialized tools, however, are usually only available in advanced research centers or in institutions that are traditionally involved with the development of novel teaching/learning processes, and are far from becoming reality in the majority of life sciences schools. When combined with the known declining interest in science among young people, a critical scenario may result. This is especially important in the field of electron microscopy and associated techniques, methods that have greatly contributed to the current knowledge on the structure and function of different cell biology models but are rarely made accessible to most students. In this work, we propose a strategy to increase the engagement of students into the world of cell and structural biology by combining 3D electron microscopy techniques and 3D prototyping technology (3D printing) to generate 3D physical models that accurately and realistically reproduce a close-to-the native structure of the cell and serve as a tool for students and teachers outside the main centers. We introduce three strategies for 3D imaging, modeling and prototyping of cells and propose the establishment of a virtual platform where different digital models can be deposited by EM groups and subsequently downloaded and printed in different schools, universities, research centers and museums, thereby modernizing teaching of cell biology and increasing the accessibility to

  2. Production of infectious genotype 1b virus particles in cell culture and impairment by replication enhancing mutations.

    Directory of Open Access Journals (Sweden)

    Thomas Pietschmann

    2009-06-01

    Full Text Available With the advent of subgenomic hepatitis C virus (HCV replicons, studies of the intracellular steps of the viral replication cycle became possible. These RNAs are capable of self-amplification in cultured human hepatoma cells, but save for the genotype 2a isolate JFH-1, efficient replication of these HCV RNAs requires replication enhancing mutations (REMs, previously also called cell culture adaptive mutations. These mutations cluster primarily in the central region of non-structural protein 5A (NS5A, but may also reside in the NS3 helicase domain or at a distinct position in NS4B. Most efficient replication has been achieved by combining REMs residing in NS3 with distinct REMs located in NS4B or NS5A. However, in spite of efficient replication of HCV genomes containing such mutations, they do not support production of infectious virus particles. By using the genotype 1b isolate Con1, in this study we show that REMs interfere with HCV assembly. Strongest impairment of virus formation was found with REMs located in the NS3 helicase (E1202G and T1280I as well as NS5A (S2204R, whereas a highly adaptive REM in NS4B still allowed virus production although relative levels of core release were also reduced. We also show that cells transfected with the Con1 wild type genome or the genome containing the REM in NS4B release HCV particles that are infectious both in cell culture and in vivo. Our data provide an explanation for the in vitro and in vivo attenuation of cell culture adapted HCV genomes and may open new avenues for the development of fully competent culture systems covering the therapeutically most relevant HCV genotypes.

  3. Identification of BC005512 as a DNA damage responsive murine endogenous retrovirus of GLN family involved in cell growth regulation.

    Directory of Open Access Journals (Sweden)

    Yuanfeng Wu

    Full Text Available Genotoxicity assessment is of great significance in drug safety evaluation, and microarray is a useful tool widely used to identify genotoxic stress responsive genes. In the present work, by using oligonucleotide microarray in an in vivo model, we identified an unknown gene BC005512 (abbreviated as BC, official full name: cDNA sequence BC005512, whose expression in mouse liver was specifically induced by seven well-known genotoxins (GTXs, but not by non-genotoxins (NGTXs. Bioinformatics revealed that BC was a member of the GLN family of murine endogenous retrovirus (ERV. However, the relationship to genotoxicity and the cellular function of GLN are largely unknown. Using NIH/3T3 cells as an in vitro model system and quantitative real-time PCR, BC expression was specifically induced by another seven GTXs, covering diverse genotoxicity mechanisms. Additionally, dose-response and linear regression analysis showed that expression level of BC in NIH/3T3 cells strongly correlated with DNA damage, measured using the alkaline comet assay,. While in p53 deficient L5178Y cells, GTXs could not induce BC expression. Further functional studies using RNA interference revealed that down-regulation of BC expression induced G1/S phase arrest, inhibited cell proliferation and thus suppressed cell growth in NIH/3T3 cells. Together, our results provide the first evidence that BC005512, a member from GLN family of murine ERV, was responsive to DNA damage and involved in cell growth regulation. These findings could be of great value in genotoxicity predictions and contribute to a deeper understanding of GLN biological functions.

  4. Expression of WNT genes in cervical cancer-derived cells: Implication of WNT7A in cell proliferation and migration

    Energy Technology Data Exchange (ETDEWEB)

    Ramos-Solano, Moisés, E-mail: mrsolano84@gmail.com [División de Inmunología, Centro de Investigación Biomédica de Occidente (CIBO)-Instituto Mexicano del Seguro Social (IMSS), Guadalajara, Jalisco (Mexico); Programa de Doctorado en Ciencias Biomédica, Centro Universitario de Ciencias de la Salud (CUCS), Universidad de Guadalajara, Guadalajara, Jalisco (Mexico); Meza-Canales, Ivan D., E-mail: imezacanales@ice.mpg.de [Department of Molecular Ecology, Max Planck Institute for Chemical Ecology, 07745 Jena (Germany); Torres-Reyes, Luis A., E-mail: torres_reyes_88@hotmail.com [División de Inmunología, Centro de Investigación Biomédica de Occidente (CIBO)-Instituto Mexicano del Seguro Social (IMSS), Guadalajara, Jalisco (Mexico); Programa de Doctorado en Ciencias Biomédica, Centro Universitario de Ciencias de la Salud (CUCS), Universidad de Guadalajara, Guadalajara, Jalisco (Mexico); Alvarez-Zavala, Monserrat, E-mail: monse_belan@hotmail.com [División de Inmunología, Centro de Investigación Biomédica de Occidente (CIBO)-Instituto Mexicano del Seguro Social (IMSS), Guadalajara, Jalisco (Mexico); Programa de Doctorado en Ciencias Biomédica, Centro Universitario de Ciencias de la Salud (CUCS), Universidad de Guadalajara, Guadalajara, Jalisco (Mexico); and others

    2015-07-01

    According to the multifactorial model of cervical cancer (CC) causation, it is now recognized that other modifications, in addition to Human papillomavirus (HPV) infection, are necessary for the development of this neoplasia. Among these, it has been proposed that a dysregulation of the WNT pathway might favor malignant progression of HPV-immortalized keratinocytes. The aim of this study was to identify components of the WNT pathway differentially expressed in CC vs. non-tumorigenic, but immortalized human keratinocytes. Interestingly, WNT7A expression was found strongly downregulated in cell lines and biopsies derived from CC. Restoration of WNT7A in CC-derived cell lines using a lentiviral gene delivery system or after adding a recombinant human protein decreases cell proliferation. Likewise, WNT7A silencing in non-tumorigenic cells markedly accelerates proliferation. Decreased WNT7A expression was due to hypermethylation at particular CpG sites. To our knowledge, this is the first study reporting reduced WNT7A levels in CC-derived cells and that ectopic WNT7A restoration negatively affects cell proliferation and migration. - Highlights: • WNT7A is expressed in normal keratinocytes or cervical cells without lesion. • WNT7A is significantly reduced in cervical cancer-derived cells. • Restoration of WNT7A expression in HeLa decreases proliferation and cell migration. • Silencing of WNT7A in HaCaT induces an increased proliferation and migration rate. • Decreased WNT7A expression in this model is due to hypermethylation.

  5. Stable isotope labeling by amino acids in cell culture (SILAC) and quantitative comparison of the membrane proteomes of self-renewing and differentiating human embryonic stem cells

    DEFF Research Database (Denmark)

    Prokhorova, Tatyana A; Rigbolt, Kristoffer T G; Johansen, Pia T

    2009-01-01

    Stable isotope labeling by amino acids in cell culture (SILAC) is a powerful quantitative proteomics platform for comprehensive characterization of complex biological systems. However, the potential of SILAC-based approaches has not been fully utilized in human embryonic stem cell (hESC) research...... embryonic stem cell lines. Of the 811 identified membrane proteins, six displayed significantly higher expression levels in the undifferentiated state compared with differentiating cells. This group includes the established marker CD133/Prominin-1 as well as novel candidates for hESC surface markers......: Glypican-4, Neuroligin-4, ErbB2, receptor-type tyrosine-protein phosphatase zeta (PTPRZ), and Glycoprotein M6B. Our study also revealed 17 potential markers of hESC differentiation as their corresponding protein expression levels displayed a dramatic increase in differentiated embryonic stem cell...

  6. Formation of zinc-containing nanoparticles from Zn²⁺ ions in cell culture media: implications for the nanotoxicology of ZnO.

    Science.gov (United States)

    Turney, Terence W; Duriska, Martin B; Jayaratne, Vidura; Elbaz, Abdulkareem; O'Keefe, Sean J; Hastings, Andrew S; Piva, Terrence J; Wright, Paul F A; Feltis, Bryce N

    2012-10-15

    Zinc ions generate a range of poorly soluble Zn-containing nanoparticles when added to commonly used mammalian cell culture media. The formation of these nanoparticles confounds the use of soluble Zn salts as positive controls during cytotoxicity testing of other Zn-containing nanoparticles, such as ZnO. These nanoprecipitates can either be crystalline or amorphous and vary in composition depending upon the concentration of Zn(II) within the medium. The cytotoxicity and immune system response of these nanoparticles in situ are similar to those of 30 nm ZnO nanoparticles. The low residual level of truly soluble Zn species (taken as species passing through a 2 kDa membrane) in cell culture media with serum is insufficient to elicit any appreciable cytotoxicity. These observations highlight the importance of employing appropriate controls when studying ZnO nanoparticle toxicity and suggest a re-evaluation of the conclusions drawn in some previous cytotoxicity studies.

  7. Canonical symplectic particle-in-cell method for long-term large-scale simulations of the Vlasov–Maxwell equations

    Energy Technology Data Exchange (ETDEWEB)

    Qin, Hong; Liu, Jian; Xiao, Jianyuan; Zhang, Ruili; He, Yang; Wang, Yulei; Sun, Yajuan; Burby, Joshua W.; Ellison, Leland; Zhou, Yao

    2015-12-14

    Particle-in-cell (PIC) simulation is the most important numerical tool in plasma physics. However, its long-term accuracy has not been established. To overcome this difficulty, we developed a canonical symplectic PIC method for the Vlasov-Maxwell system by discretising its canonical Poisson bracket. A fast local algorithm to solve the symplectic implicit time advance is discovered without root searching or global matrix inversion, enabling applications of the proposed method to very large-scale plasma simulations with many, e.g. 10(9), degrees of freedom. The long-term accuracy and fidelity of the algorithm enables us to numerically confirm Mouhot and Villani's theory and conjecture on nonlinear Landau damping over several orders of magnitude using the PIC method, and to calculate the nonlinear evolution of the reflectivity during the mode conversion process from extraordinary waves to Bernstein waves.

  8. Modeling Ebola Virus Genome Replication and Transcription with Minigenome Systems.

    Science.gov (United States)

    Cressey, Tessa; Brauburger, Kristina; Mühlberger, Elke

    2017-01-01

    In this chapter, we describe the minigenome system for Ebola virus (EBOV), which reconstitutes EBOV polymerase activity in cells and can be used to model viral genome replication and transcription. This protocol comprises all steps including cell culture, plasmid preparation, transfection, and luciferase reporter assay readout.

  9. Coupling component systems towards systems of systems

    OpenAIRE

    Autran , Frédéric; Auzelle , Jean-Philippe; Cattan , Denise; Garnier , Jean-Luc; Luzeaux , Dominique; Mayer , Frédérique; Peyrichon , Marc; Ruault , Jean-René

    2008-01-01

    International audience; Systems of systems (SoS) are a hot topic in our "fully connected global world". Our aim is not to provide another definition of what SoS are, but rather to focus on the adequacy of reusing standard system architecting techniques within this approach in order to improve performance, fault detection and safety issues in large-scale coupled systems that definitely qualify as SoS, whatever the definition is. A key issue will be to secure the availability of the services pr...

  10. Systems effectiveness

    CERN Document Server

    Habayeb, A R

    1987-01-01

    Highlights three principal applications of system effectiveness: hardware system evaluation, organizational development and evaluation, and conflict analysis. The text emphasizes the commonality of the system effectiveness discipline. The first part of the work presents a framework for system effectiveness, partitioning and hierarchy of hardware systems. The second part covers the structure, hierarchy, states, functions and activities of organizations. Contains an extended Appendix on mathematical concepts and also several project suggestions.

  11. Auxiliary systems

    International Nuclear Information System (INIS)

    Meyer, P.J.

    1981-01-01

    Systems included under the heading ''Reactor Auxillary Systems'' are those immediately involved with the reactor operation. These include the systems for dosing and letdown of reactor coolant, as well as for the chemical dosing, purification and treatment of the reactor coolant and the cooling system in the controlled area. The ancillary systems are mainly responsible for liquid and gaseous treatment and the waste treatment for final storage. (orig.)

  12. FLIP-MHD: A particle-in-cell mehtod for magnetohydrodynamics

    International Nuclear Information System (INIS)

    Brackbill, J.U.

    1990-01-01

    A particle-in-cell (PIC) method, FLIP is extended to magnetohydrodynamic (MHD) flow in two dimensions. Particles are used to reduce computational diffusion of the magnetic field. FLIP is an extension of ''classical'' PIC, where particles have mass, but every other property of the fluid is stored on a grid. In FLIP, particles have every property of the fluid, so that they provide a complete Lagrangian description not only to resolve contact discontinuities but also to reduce computational diffusion of linear and angular momentum. The interactions among the particles are calculated on a grid, for convenience and economy. The present study extends FLIP to MHD, by including information about the magnetic field among the attributes of the particles. 6 refs

  13. A 2-D Implicit, Energy and Charge Conserving Particle In Cell Method

    International Nuclear Information System (INIS)

    McPherson, Allen L.; Knoll, Dana A.; Cieren, Emmanuel B.; Feltman, Nicolas; Leibs, Christopher A.; McCarthy, Colleen; Murthy, Karthik S.; Wang, Yijie

    2012-01-01

    Recently, a fully implicit electrostatic 1D charge- and energy-conserving particle-in-cell algorithm was proposed and implemented by Chen et al ([2],[3]). Central to the algorithm is an advanced particle pusher. Particles are moved using an energy conserving scheme and are forced to stop at cell faces to conserve charge. Moreover, a time estimator is used to control errors in momentum. Here we implement and extend this advanced particle pusher to include 2D and electromagnetic fields. Derivations of all modifications made are presented in full. Special consideration is taken to ensure easy coupling into the implicit moment based method proposed by Taitano et al [19]. Focus is then given to optimizing the presented particle pusher on emerging architectures. Two multicore implementations, and one GPU (Graphics Processing Unit) implementation are discussed and analyzed.

  14. The physical and functional interaction of NDRG2 with MSP58 in cells

    International Nuclear Information System (INIS)

    Zhang Jing; Liu Junye; Li Xia; Li Fuyang; Wang Lifeng; Zhang Jian; Liu Xinping; Shen Lan; Liu Na; Deng Yanchun; Yang Angang; Han Hua; Zhao Mujun; Yao Libo

    2007-01-01

    NDRG2, a member of N-Myc downstream regulated gene family, exerts the important functions in cell differentiation and tumor suppression. Although the ectopic expressed Ndrg2 inhibits the proliferation of tumor cells, its intracellular signal transduction pathway is hardly known. Here, we identified MSP58, a 58-kDa microspherule protein, as an interacting partner of human Ndrg2 by using yeast two-hybrid screening. The interaction was confirmed by glutathione S-transferase pull-down assay in vitro and by co-immune-precipitation assay in vivo. The forkhead associated domain of MSP58 is essential for its interaction with Ndrg2. Ndrg2 could co-localize with MSP58 in nuclear of HeLa cell during cell stress. Furthermore, the modulation of Ndrg2 level influences the cell cycle process together with MSP58. In conclusion, the findings offered a novel insight into the physiological roles of Ndrg2

  15. Particle-in-cell simulation of Trichel pulses in pure oxygen

    International Nuclear Information System (INIS)

    Soria-Hoyo, C; Pontiga, F; Castellanos, A

    2007-01-01

    The development and propagation of Trichel pulses in oxygen have been numerically simulated using an improved fluid particle-in-cell (PIC) method. The numerical method has been optimized to accurately reproduce sequences of about 100 Trichel pulses (∼1 ms). A classical one-dimensional model of negative corona in sphere-to-plane geometry has been used to formulate the continuity equations for electrons and ions. The effects of ionization, attachment and secondary-electron emission from the cathode have all been considered. The electric field has been obtained from the solution of Poisson's equation in two dimensions. Using this model, the temporal and electrical characteristics of Trichel pulses have been investigated, in particular, the relation between applied voltage, pulse frequency and time-averaged current intensity and charge

  16. Two dimensional numerical simulation of gas discharges: comparison between particle-in-cell and FCT techniques

    Energy Technology Data Exchange (ETDEWEB)

    Soria-Hoyo, C; Castellanos, A [Departamento de Electronica y Electromagnetismo, Facultad de Fisica, Universidad de Sevilla, Avda. Reina Mercedes s/n, 41012 Sevilla (Spain); Pontiga, F [Departamento de Fisica Aplicada II, EUAT, Universidad de Sevilla, Avda. Reina Mercedes s/n, 41012 Sevilla (Spain)], E-mail: cshoyo@us.es

    2008-10-21

    Two different numerical techniques have been applied to the numerical integration of equations modelling gas discharges: a finite-difference flux corrected transport (FD-FCT) technique and a particle-in-cell (PIC) technique. The PIC technique here implemented has been specifically designed for the simulation of 2D electrical discharges using cylindrical coordinates. The development and propagation of a streamer between two parallel electrodes has been used as a convenient test to compare the performance of both techniques. In particular, the phase velocity of the cathode directed streamer has been used to check the internal consistency of the numerical simulations. The results obtained from the two techniques are in reasonable agreement with each other, and both techniques have proved their ability to follow the high gradients of charge density and electric field present in this type of problems. Moreover, the streamer velocities predicted by the simulation are in accordance with the typical experimental values.

  17. Particle-in-cell simulation of Trichel pulses in pure oxygen

    Energy Technology Data Exchange (ETDEWEB)

    Soria-Hoyo, C [Dpto. Electronica y Electromagnetismo, Universidad de Sevilla, Avda. Reina Mercedes s/n, Sevilla 41012 (Spain); Pontiga, F [Dpto. Fisica Aplicada II, Universidad de Sevilla, Avda. Reina Mercedes s/n, Sevilla 41012 (Spain); Castellanos, A [Dpto. Electronica y Electromagnetismo, Universidad de Sevilla, Avda. Reina Mercedes s/n, Sevilla 41012 (Spain)

    2007-08-07

    The development and propagation of Trichel pulses in oxygen have been numerically simulated using an improved fluid particle-in-cell (PIC) method. The numerical method has been optimized to accurately reproduce sequences of about 100 Trichel pulses ({approx}1 ms). A classical one-dimensional model of negative corona in sphere-to-plane geometry has been used to formulate the continuity equations for electrons and ions. The effects of ionization, attachment and secondary-electron emission from the cathode have all been considered. The electric field has been obtained from the solution of Poisson's equation in two dimensions. Using this model, the temporal and electrical characteristics of Trichel pulses have been investigated, in particular, the relation between applied voltage, pulse frequency and time-averaged current intensity and charge.

  18. Two dimensional numerical simulation of gas discharges: comparison between particle-in-cell and FCT techniques

    International Nuclear Information System (INIS)

    Soria-Hoyo, C; Castellanos, A; Pontiga, F

    2008-01-01

    Two different numerical techniques have been applied to the numerical integration of equations modelling gas discharges: a finite-difference flux corrected transport (FD-FCT) technique and a particle-in-cell (PIC) technique. The PIC technique here implemented has been specifically designed for the simulation of 2D electrical discharges using cylindrical coordinates. The development and propagation of a streamer between two parallel electrodes has been used as a convenient test to compare the performance of both techniques. In particular, the phase velocity of the cathode directed streamer has been used to check the internal consistency of the numerical simulations. The results obtained from the two techniques are in reasonable agreement with each other, and both techniques have proved their ability to follow the high gradients of charge density and electric field present in this type of problems. Moreover, the streamer velocities predicted by the simulation are in accordance with the typical experimental values.

  19. Electron and ion heating by whistler turbulence: Three-dimensional particle-in-cell simulations

    International Nuclear Information System (INIS)

    Hughes, R. Scott; Gary, S. Peter; Wang, Joseph

    2014-01-01

    Three-dimensional particle-in-cell simulations of decaying whistler turbulence are carried out on a collisionless, homogeneous, magnetized, electron-ion plasma model. In addition, the simulations use an initial ensemble of relatively long wavelength whistler modes with a broad range of initial propagation directions with an initial electron beta β e = 0.05. The computations follow the temporal evolution of the fluctuations as they cascade into broadband turbulent spectra at shorter wavelengths. Three simulations correspond to successively larger simulation boxes and successively longer wavelengths of the initial fluctuations. The computations confirm previous results showing electron heating is preferentially parallel to the background magnetic field B o , and ion heating is preferentially perpendicular to B o . The new results here are that larger simulation boxes and longer initial whistler wavelengths yield weaker overall dissipation, consistent with linear dispersion theory predictions of decreased damping, stronger ion heating, consistent with a stronger ion Landau resonance, and weaker electron heating

  20. A Generalized Weight-Based Particle-In-Cell Simulation Scheme

    International Nuclear Information System (INIS)

    Lee, W.W.; Jenkins, T.G.; Ethier, S.

    2010-01-01

    A generalized weight-based particle simulation scheme suitable for simulating magnetized plasmas, where the zeroth-order inhomogeneity is important, is presented. The scheme is an extension of the perturbative simulation schemes developed earlier for particle-in-cell (PIC) simulations. The new scheme is designed to simulate both the perturbed distribution ((delta)f) and the full distribution (full-F) within the same code. The development is based on the concept of multiscale expansion, which separates the scale lengths of the background inhomogeneity from those associated with the perturbed distributions. The potential advantage for such an arrangement is to minimize the particle noise by using (delta)f in the linear stage stage of the simulation, while retaining the flexibility of a full-F capability in the fully nonlinear stage of the development when signals associated with plasma turbulence are at a much higher level than those from the intrinsic particle noise.