WorldWideScience

Sample records for in-cell material-handling system

  1. Bulk material handling system

    Science.gov (United States)

    Kleysteuber, William K.; Mayercheck, William D.

    1979-01-01

    This disclosure relates to a bulk material handling system particularly adapted for underground mining and includes a monorail supported overhead and carrying a plurality of conveyors each having input and output end portions with the output end portion of a first of the conveyors positioned above an input end portion of a second of the conveyors, a device for imparting motion to the conveyors to move the material from the input end portions toward the output end portions thereof, a device for supporting at least one of the input and output end portions of the first and second conveyors from the monorail, and the supporting device including a plurality of trolleys rollingly supported by the monorail whereby the conveyors can be readily moved therealong.

  2. Techniques for remote maintenance of in-cell material-handling system in the HFEF/N main cell

    International Nuclear Information System (INIS)

    Tobias, D.A.; Frickey, C.A.

    1975-01-01

    Operations in the main cell of HFEF/N have required development of remote handling equipment and unique techniques for maintaining the in-cell material-handling system. Specially designed equipment is used to remove a disabled crane or electromechanical manipulator bridge from its support rails and place it on floor stands for repair or maintenance. Support areas for the main cell, such as the spray chamber and hot repair area, provide essential decontamination, repair, and staging areas for the in-cell material-handling-system equipment and tools. A combined engineering and technical effort in upgrading existing master-slave manipulators has definitely reduced the requirements for their maintenance. The cell is primarily for postirradiation examination of LMFBR materials and fuel elements

  3. Lunar Materials Handling System, Phase II

    Data.gov (United States)

    National Aeronautics and Space Administration — The Lunar Materials Handling System (LMHS) is a method for transfer of lunar soil into and out of process equipment in support of in situ resource utilization...

  4. Lunar Materials Handling System, Phase I

    Data.gov (United States)

    National Aeronautics and Space Administration — The Lunar Materials Handling System (LMHS) is a method for transfer of bulk materials and products into and out of process equipment in support of lunar and Mars in...

  5. MHSS: a material handling system simulator

    Energy Technology Data Exchange (ETDEWEB)

    Pomernacki, L.; Hollstien, R.B.

    1976-04-07

    A Material Handling System Simulator (MHSS) program is described that provides specialized functional blocks for modeling and simulation of nuclear material handling systems. Models of nuclear fuel fabrication plants may be built using functional blocks that simulate material receiving, storage, transport, inventory, processing, and shipping operations as well as the control and reporting tasks of operators or on-line computers. Blocks are also provided that allow the user to observe and gather statistical information on the dynamic behavior of simulated plants over single or replicated runs. Although it is currently being developed for the nuclear materials handling application, MHSS can be adapted to other industries in which material accountability is important. In this paper, emphasis is on the simulation methodology of the MHSS program with application to the nuclear material safeguards problem. (auth)

  6. MHSS: a material handling system simulator

    International Nuclear Information System (INIS)

    Pomernacki, L.; Hollstien, R.B.

    1976-01-01

    A Material Handling System Simulator (MHSS) program is described that provides specialized functional blocks for modeling and simulation of nuclear material handling systems. Models of nuclear fuel fabrication plants may be built using functional blocks that simulate material receiving, storage, transport, inventory, processing, and shipping operations as well as the control and reporting tasks of operators or on-line computers. Blocks are also provided that allow the user to observe and gather statistical information on the dynamic behavior of simulated plants over single or replicated runs. Although it is currently being developed for the nuclear materials handling application, MHSS can be adapted to other industries in which material accountability is important. In this paper, emphasis is on the simulation methodology of the MHSS program with application to the nuclear material safeguards problem

  7. Generic control of material handling systems

    NARCIS (Netherlands)

    Haneyah, S.W.A.

    2013-01-01

    Material handling systems (MHSs) are in general complex installations that raise challenging design and control problems. In the literature, design and control problems have received a lot of attention within distinct business sectors or systems, but primarily from a system’s user perspective. Much

  8. A Knowledge-Based Approach for Selection of Material Handling Equipment and Material Handling System Pre-design

    OpenAIRE

    YAMAN, Ramazan

    2014-01-01

    For material handling system design, material handling equipment selection is the first stage. Also the material handling system and facility layout design problems are coupled. Solving these problems needs consideration of these three different problems. Right material handling equipment selection and good design of the material handling system and facility layout can increase productivity and reduce investments and operations' costs. In this study, after describing the m...

  9. Aspects of materials handling systems in the steel fabrication environment

    OpenAIRE

    2012-01-01

    M.Ing. This dissertation covers a literature survey of materials handling and plant layout in the steel fabrication environment. It covers some of the elements and principles of materials handling and how they interact with and influence the effective flow of materials in an operation. Some of the current materials handling methods and systems of a specific South African steel fabrication operation are then investigated. By using the information from the literature study, an evaluation is ...

  10. EQUIPMENT PRESELECTION FOR INTEGRATED DESIGN OF MATERIALS HANDLING SYSTEMS

    OpenAIRE

    PÉTER TELEK

    2013-01-01

    There is no universal solution method for the design of materials handling systems, which can be applied in all of the system varieties. Probably the best method is the using of an integrated design process, but it can also be problematic in certain cases. In this paper I show a new design concept which can be easily adapted in a simple or in a complex materials handling system. The first step of the process is a preselection of the devices which enables the selection of the applicable materi...

  11. Modelling dust liberation in bulk material handling systems

    NARCIS (Netherlands)

    Derakhshani, S.M.

    2016-01-01

    Dust has negative effects on the environmental conditions, human health as well as industrial equipment and processes. In this thesis, the transfer point of a belt conveyor as a bulk material handling system with a very high potential place for dust liberation is studied. This study is conducted

  12. Development and implementation of automated radioactive materials handling systems

    International Nuclear Information System (INIS)

    Jacoboski, D.L.

    1992-12-01

    Material handling of radioactive and hazardous materials has forced the need to pursue remotely operated and robotic systems in light of operational safety concerns. Manual maneuvering, repackaging, overpacking and inspecting of containers which store radioactive and hazardous materials is the present mode of operation at the Department of Energy (DOE) Fernald Environmental Management Project (FEMP) in Fernald Ohio. The manual methods are unacceptable in the eyes of concerned site workers and influential community oversight committees. As an example to respond to the FEMP material handling needs, design efforts have been initiated to provide a remotely operated system to repackage thousands of degradated drums containing radioactive Thorium: Later, the repackaged Thorium will be shipped offsite to a predesignated repository again requiring remote operation

  13. CLASSIFICATION OF THE MGR CARRIER PREPARATION BUILDING MATERIALS HANDLING SYSTEM

    International Nuclear Information System (INIS)

    J.A. Ziegler

    2001-01-01

    The purpose of this analysis is to document the Quality Assurance (QA) classification of the Monitored Geologic Repository (MGR) carrier preparation building materials handling system structures, systems and components (SSCs) performed by the MGR Preclosure Safety and Systems Engineering Section. This analysis also provides the basis for revision of YMP/90-55Q, Q-List (YMP 2000). The Q-List identifies those MGR SSCs subject to the requirements of DOE/RW-0333P7 ''Quality Assurance Requirements and Description'' (QARD) (DOE 2000)

  14. CARRIER PREPARATION BUILDING MATERIALS HANDLING SYSTEM DESCRIPTION DOCUMENT

    Energy Technology Data Exchange (ETDEWEB)

    E.F. Loros

    2000-06-28

    The Carrier Preparation Building Materials Handling System receives rail and truck shipping casks from the Carrier/Cask Transport System, and inspects and prepares the shipping casks for return to the Carrier/Cask Transport System. Carrier preparation operations for carriers/casks received at the surface repository include performing a radiation survey of the carrier and cask, removing/retracting the personnel barrier, measuring the cask temperature, removing/retracting the impact limiters, removing the cask tie-downs (if any), and installing the cask trunnions (if any). The shipping operations for carriers/casks leaving the surface repository include removing the cask trunnions (if any), installing the cask tie-downs (if any), installing the impact limiters, performing a radiation survey of the cask, and installing the personnel barrier. There are four parallel carrier/cask preparation lines installed in the Carrier Preparation Building with two preparation bays in each line, each of which can accommodate carrier/cask shipping and receiving. The lines are operated concurrently to handle the waste shipping throughputs and to allow system maintenance operations. One remotely operated overhead bridge crane and one remotely operated manipulator is provided for each pair of carrier/cask preparation lines servicing four preparation bays. Remotely operated support equipment includes a manipulator and tooling and fixtures for removing and installing personnel barriers, impact limiters, cask trunnions, and cask tie-downs. Remote handling equipment is designed to facilitate maintenance, dose reduction, and replacement of interchangeable components where appropriate. Semi-automatic, manual, and backup control methods support normal, abnormal, and recovery operations. Laydown areas and equipment are included as required for transportation system components (e.g., personnel barriers and impact limiters), fixtures, and tooling to support abnormal and recovery operations. The

  15. Radial Internal Material Handling System (RIMS) for Circular Habitat Volumes

    Science.gov (United States)

    Howe, Alan S.; Haselschwardt, Sally; Bogatko, Alex; Humphrey, Brian; Patel, Amit

    2013-01-01

    On planetary surfaces, pressurized human habitable volumes will require a means to carry equipment around within the volume of the habitat, regardless of the partial gravity (Earth, Moon, Mars, etc.). On the NASA Habitat Demonstration Unit (HDU), a vertical cylindrical volume, it was determined that a variety of heavy items would need to be carried back and forth from deployed locations to the General Maintenance Work Station (GMWS) when in need of repair, and other equipment may need to be carried inside for repairs, such as rover parts and other external equipment. The vertical cylindrical volume of the HDU lent itself to a circular overhead track and hoist system that allows lifting of heavy objects from anywhere in the habitat to any other point in the habitat interior. In addition, the system is able to hand-off lifted items to other material handling systems through the side hatches, such as through an airlock. The overhead system consists of two concentric circle tracks that have a movable beam between them. The beam has a hoist carriage that can move back and forth on the beam. Therefore, the entire system acts like a bridge crane curved around to meet itself in a circle. The novelty of the system is in its configuration, and how it interfaces with the volume of the HDU habitat. Similar to how a bridge crane allows coverage for an entire rectangular volume, the RIMS system covers a circular volume. The RIMS system is the first generation of what may be applied to future planetary surface vertical cylinder habitats on the Moon or on Mars.

  16. Learning to Design and Analyze Materials Handling Systems: Developing Multimedia Tools

    Science.gov (United States)

    Heragu, Sunderesh; Jennings, Sybillyn

    2003-01-01

    In this paper, we describe aspects related to learning and learning assessment including pedagogy, cognition, pilot study and results from the study. This study is conducted for an educational module on "10 Principles of Materials Handling". This module along with another on "Analysis and Design of Integrated Materials Handling Systems" constitute…

  17. Improvement Of Physical Ergonomics Using Material Handling Systems

    Directory of Open Access Journals (Sweden)

    Naveen Kumar

    2017-01-01

    Full Text Available This research paper is an investigation of the physical ergonomics of the work place in an automotive parts manufacturing company . Material transfer from one station to another station was done by hand including a walk of a few steps to the next station. The unmachined components that has a quite heavy raw weight also they are being loaded and unloaded by hand .Due to this continuous practice some workers began complaining physical pain in their backs and muscular related pains. The work conditions of the workers were assessed using the REBA Rapid Entire Body Assessment test to understand the stress and the impact the work environment they are exposed to. Few material handling concepts have been suggested and explained to improve the quality of the work conditions for the workers and the REBA test tends to show some significant improvement when these improvements are implemented into the production line.

  18. The use of physical model simulation to emulate an AGV material handling system

    International Nuclear Information System (INIS)

    Hurley, R.G.; Coffman, P.E.; Dixon, J.R.; Walacavage, J.G.

    1987-01-01

    This paper describes an application of physical modeling to the simulation of a prototype AGV (Automatic Guided Vehicle) material handling system. Physical modeling is the study of complex automated manufacturing and material handling systems through the use of small scale components controlled by mini and/or microcomputers. By modeling the mechanical operations of the proposed AGV material handling system, it was determined that control algorithms and AGV dispatch rules could be developed and evaluated. This paper presents a brief explanation of physical modeling as a simulation tool and addresses in detail the development of the control algorithm, dispatching rules, and a prototype physical model of a flexible machining system

  19. A generic control architecture for material handling systems applied to a baggage handling system

    NARCIS (Netherlands)

    Haneyah, S. W A; Schutten, J. M J; Schuur, P. C.; Zijm, W. H M

    2013-01-01

    This paper is part of research on generic planning and control of automated Material Handling Systems (MHSs) in different industrial sectors. We build upon previous work to provide a proof of concept for the applicability of a generic control architecture on a specific MHS. To this end, the baggage

  20. Material handling systems for the fluidized-bed combustion boiler at Rivesville, West Virginia

    Science.gov (United States)

    Branam, J. G.; Rosborough, W. W.

    1977-01-01

    The 300,000 lbs/hr steam capacity multicell fluidized-bed boiler (MFB) utilizes complex material handling systems. The material handling systems can be divided into the following areas: (1) coal preparation; transfer and delivery, (2) limestone handling system, (3) fly-ash removal and (4) bed material handling system. Each of the above systems are described in detail and some of the potential problem areas are discussed. A major potential problem that exists is the coal drying system. The coal dryer is designed to use 600 F preheated combustion air as drying medium and the dryer effluent is designed to enter a hot electrostatic precipitator (730 F) after passage through a cyclone. Other problem areas to be discussed include the steam generator coal and limestone feed system which may have operating difficulties with wet coal and/or coal fines.

  1. Planning and Control Concepts for Material Handling Systems

    NARCIS (Netherlands)

    I.F.A. Vis (Iris)

    2002-01-01

    textabstractIris Vis was born in 1974 in Leidschendam. May 2002 - Assistant professor at the School of Economics and Business Administration, Vrije Universiteit Amsterdam 1999, Visiting scholar at Georgia Institute of Technology, School of Industrial and Systems Engineering, october -

  2. Contamination confinement system of irradiated materials handling laboratories

    International Nuclear Information System (INIS)

    Lobao, A. dos S.T.; Araujo, J.A. de; Camilo, R.L.

    1988-06-01

    A study to prevent radioctivity release in lab scale is presented. As a basis for the design all the limits established by the IAEA for ventilation systems were observed. An evaluation of the different parameters involved in the design have been made, resulting in the especification of the working areas, ducts and filtering systems in order to get the best conditions for the safe handling of irradiated materials. (author) [pt

  3. Robotic control architecture development for automated nuclear material handling systems

    International Nuclear Information System (INIS)

    Merrill, R.D.; Hurd, R.; Couture, S.; Wilhelmsen, K.

    1995-02-01

    Lawrence Livermore National Laboratory (LLNL) is engaged in developing automated systems for handling materials for mixed waste treatment, nuclear pyrochemical processing, and weapon components disassembly. In support of these application areas there is an extensive robotic development program. This paper will describe the portion of this effort at LLNL devoted to control system architecture development, and review two applications currently being implemented which incorporate these technologies

  4. Robotic control architecture development for automated nuclear material handling systems

    Energy Technology Data Exchange (ETDEWEB)

    Merrill, R.D.; Hurd, R.; Couture, S.; Wilhelmsen, K.

    1995-02-01

    Lawrence Livermore National Laboratory (LLNL) is engaged in developing automated systems for handling materials for mixed waste treatment, nuclear pyrochemical processing, and weapon components disassembly. In support of these application areas there is an extensive robotic development program. This paper will describe the portion of this effort at LLNL devoted to control system architecture development, and review two applications currently being implemented which incorporate these technologies.

  5. Pilot material handling system for radiation processing of agricultural and medical products

    International Nuclear Information System (INIS)

    Sandha, R.S.; Nageswar Rao, J; Dwivedi, Jishnu; Petwal, V.C.; Soni, H.C.

    2005-01-01

    A 10 MeV, 10 kW electron LINAC based radiation processing facility is being constructed at Centre for Advanced Technology, Indore for radiation processing of various food products like potatoes, onion, spices, home pack items and medical sterilization. A pilot material handling system has been designed, manufactured, and installed at CAT to verify process parameters viz. conveying speed, dose uniformity, and to study the effect of packing shape and size for radiation processing of different product. This paper describes various features of pilot material handling system. (author)

  6. Generic planning and control of automated material handling systems : practical requirements versus existing theory

    NARCIS (Netherlands)

    Haneyah, S.W.A.; Schutten, Johannes M.J.; Schuur, Peter; Zijm, Willem H.M.

    2013-01-01

    This paper discusses the problem to design a generic planning and control architecture for utomated material handling systems (AMHSs). We illustrate the relevance of this research direction, and then address three different market sectors where AMHSs are used, i.e., baggage handling, distribution,

  7. Planning and control of automated material handling systems: The merge module

    NARCIS (Netherlands)

    Haneyah, S.W.A.; Hurink, Johann L.; Schutten, Johannes M.J.; Zijm, Willem H.M.; Schuur, Peter; Hu, Bo; Morasch, Karl; Pickl, Stefan; Siegle, Markus

    2011-01-01

    We address the field of internal logistics, embodied in Automated Material Handling Systems (AMHSs), which are complex installations employed in sectors such as Baggage Handling, Physical Distribution, and Parcel & Postal. We work on designing an integral planning and real-time control architecture,

  8. Generic Planning and Control of Automated Material Handling Systems: Practical Requirements Versus Existing Theroy.

    NARCIS (Netherlands)

    Haneyah, S.W.A.; Zijm, Willem H.M.; Schutten, Johannes M.J.; Schuur, Peter

    2011-01-01

    This paper discusses the problem of generic planning and control of Automated Material Handling Systems (AMHSs). The paper illustrates the relevance of this research direction, and then addresses three different market sectors where AMHSs are used. These market sectors are: baggage handling,

  9. MODELING APPROACH TO SIMULTANEOUS SCHEDULING BATTERIES AND VEHICLES IN MATERIALS HANDLING SYSTEMS

    OpenAIRE

    Milorad Vidović; Branislava Ratković

    2015-01-01

    Battery operated handling equipment is the most widely applied concept in materials handling and logistic systems in general. The problem related to its application is in defining the most appropriate scheduling batteries and vehicles to handling tasks. Although the problem can be found in literature very often as very important, solution approaches are very rare and almost don’t exist. This paper presents one of possible solving approaches to the problem, considering the optimal assignment o...

  10. Design and simulation of integration system between automated material handling system and manufacturing layout in the automotive assembly line

    Science.gov (United States)

    Seha, S.; Zamberi, J.; Fairu, A. J.

    2017-10-01

    Material handling system (MHS) is an important part for the productivity plant and has recognized as an integral part of today’s manufacturing system. Currently, MHS has growth tremendously with its technology and equipment type. Based on the case study observation, the issue involving material handling system contribute to the reduction of production efficiency. This paper aims to propose a new design of integration between material handling and manufacturing layout by investigating the influences of layout and material handling system. A method approach tool using Delmia Quest software is introduced and the simulation result is used to assess the influences of the integration between material handling system and manufacturing layout in the performance of automotive assembly line. The result show, the production of assembly line output increases more than 31% from the current system. The source throughput rate average value went up to 252 units per working hour in model 3 and show the effectiveness of the pick-to-light system as efficient storage equipment. Thus, overall result shows, the application of AGV and the pick-to-light system gave a large significant effect in the automotive assembly line. Moreover, the change of layout also shows a large significant improvement to the performance.

  11. Material handling systems for use in glovebox lines: A survey of Department of Energy facility experience

    International Nuclear Information System (INIS)

    Teese, G.D.; Randall, W.J.

    1992-01-01

    The Nuclear Weapons Complex Reconfiguration Study has recommended that a new manufacturing facility be constructed to replace the Rocky Flats Plant. In the new facility, use of an automated material handling system for movement of components would reduce both the cost and radiation exposure associated with production and maintenance operations. Contamination control would be improved between process steps through the use of airlocks and portals. Part damage associated with improper transport would be reduced, and accountability would be increased. In-process workpieces could be stored in a secure vault, awaiting a request for parts at a production station. However, all of these desirable features rely on the proper implementation of an automated material handling system. The Department of Energy Weapons Production Complex has experience with a variety of methods for transporting discrete parts in glovebox lines. The authors visited several sites to evaluate the existing technologies for their suitability for the application of plutonium manufacturing. Technologies reviewed were Linear motors, belt conveyors, roller conveyors, accumulating roller conveyors, pneumatic transport, and cart systems. The sites visited were The Idaho National Engineering laboratory, the Hanford Site, and the Rocky Flats Plant. Linear motors appear to be the most promising technology observed for the movement of discrete parts, and further investigation is recommended

  12. A comparative life cycle assessment of material handling systems for sustainable mining.

    Science.gov (United States)

    Erkayaoğlu, M; Demirel, N

    2016-06-01

    In this comprehensive LCA comparison study, main objectives are to investigate life cycle environmental impacts of off-highway mining trucks and belt conveyors in surface mining. The research methodology essentially entails determination of the functional unit as 20,000 tons/day coal production transported for 5 km distance. After the system boundary was selected as the entire life cycle of material handling systems including pre-manufacturing of steel parts and plastic components, manufacturing, transportation, and utilization data was compiled from equipment manufacturers and the Eco-invent database. Life cycle impact categories for both material-handling systems were identified and the developed model was implemented using SIMAPRO 7.3. Climate change and acidification were selected as major impact categories as they were considered to be major concerns in mining industry. Although manufacturing stage had a significant impact on all of the environmental parameters, utilization stage was the hotspot for the selected impact categories. The results of this study revealed that belt conveyors have a greater environmental burden in climate change impact category when compared to the trucks. On the other hand, trucks have a greater environmental burden in acidification impact category when compared to the belt conveyors. This study implied that technological improvement in fuel combustion and electricity generation is crucial for the improvement of environmental profiles of off-highway trucks and belt conveyors in the mining industry. The main novelty of this study is that it is the first initiative in applying LCA in the Turkish mining industry. Copyright © 2016 Elsevier Ltd. All rights reserved.

  13. Improvements in robotic natural orifice surgery with a novel material handling system.

    Science.gov (United States)

    Midday, Jeff; Nelson, Carl A; Oleynikov, Dmitry

    2013-09-01

    Natural orifice translumenal endoscopic surgery (NOTES) has many potential advantages over other minimally invasive surgical techniques, but it presents a number of challenges introduced by the restrictive natural access points. Fully insertable dexterous in vivo robots have been developed that eliminate the spatial restrictions of the entry point, but they also are isolated within the abdomen. A material handling system (MHS) developed to bridge the gap between the in vivo robots and the surgical team promises a number of improvements over other current technologies. The MHS was implemented with two different nonsurvival swine models to validate the utility and benefits of the system. The first procedure was attempted transgastrically but proved too difficult because the geometry of the esophagus was prohibitively small. The system was instead inserted via a 50-mm GelPort and tested for robustness. The second procedure used a transvaginal insertion via a custom 25-mm trocar. Throughout both procedures, the practitioners were asked for qualitative feedback regarding the effectiveness of the device and its long-term efficiencies. The MHS was able to deliver a standard surgical staple securely to the peritoneal cavity. The practitioner was able to use the laparoscopic grasper both to insert and to remove the staple from the MHS. The system also proved capable of maintaining insufflation pressure throughout a procedure. It was cycled a total of five times in both the insertion and the retraction directions. Visualization from the MHS camera was poor at times because the lighting on the system was somewhat inadequate. No excessive bleeding or collateral damage to surrounding tissues was observed during the procedure. This study demonstrated that the MHS is fully capable of achieving payload transport during a NOTES operation. The system is intuitive and easy to use. It dramatically decreases collateral trauma in the natural access point and can advantageously reduce

  14. Proposal for Construction/Demonstration/Implementation of A Material Handling System

    International Nuclear Information System (INIS)

    Jim Jnatt

    2001-01-01

    Vortec Corporation, the United States Enrichment Corporation (USEC) and DOE/Paducah propose to complete the technology demonstration and the implementation of the Material Handling System developed under Contract Number DE-AC21-92MC29120. The demonstration testing and operational implementation will be done at the Paducah Gaseous Diffusion Plant. The scope of work, schedule and cost for the activities are included in this proposal. A description of the facility to be constructed and tested is provided in Exhibit 1, attached. The USEC proposal for implementation at Paducah is presented in Exhibit 2, and the commitment letters from the site are included in Exhibit 3. Under our agreements with USEC, Bechtel Jacobs Corporation and DOE/Paducah, Vortec will be responsible for the construction of the demonstration facility as documented in the engineering design package submitted under Phase 4 of this contract on August 9, 2001. USEC will have responsibility for the demonstration testing and commercial implementation of the plant. The demonstration testing and initial commercial implementation of the technology will be achieved by means of a USEC work authorization task with the Bechtel Jacobs Corporation. The initial processing activities will include the processing of approximately 4,250 drums of LLW. Subsequent processing of LLW and TSCA/LLW will be done under a separate contract or work authorization task. To meet the schedule for commercial implementation, it is important that the execution of the Phase 4 project option for construction of the demonstration system be executed as soon as possible. The schedule we have presented herein assumes initiation of the construction phase by the end of September 2001. Vortec proposes to complete construction of the demonstration test system for an estimated cost of $3,254,422. This price is based on the design submitted to DOE/NETL under the Phase 4 engineering design deliverable (9 august 2001). The cost is subject to the

  15. Performance evaluation of a wearable inertial motion capture system for capturing physical exposures during manual material handling tasks.

    Science.gov (United States)

    Kim, Sunwook; Nussbaum, Maury A

    2013-01-01

    With a long-term goal of improving quantification of physical exposures in the workplace, this study examined the ability of a commercially available inertial motion capture (IMC) system in quantifying exposures during five different simulated manual material handling tasks. Fourteen participants repeated all these tasks in three 20 min sequential time blocks. Performance of the IMC system was compared against an optical motion capture (OMC) system ('gold standard') in terms of joint angles, angular velocities and moments at selected body parts. Though several significant changes in performance over time were found, the magnitudes of these were relatively small and may have limited practical relevance. The IMC system yielded peak kinematic values that differed by up to 28% from the OMC system. The IMC system, in some cases, incorrectly reflected the actual extremity positions of a participant, and which can cause relatively large errors in joint moment estimation. Given the potential limitations, practical recommendations are offered and discussed. Use of an inertial motion capture system can advance the quantification of physical exposures in situ. Results indicate a good potential capacity for capturing physical exposure data in the field for an extended period, while highlighting potential limitations. Future system application can help provide better understandings of dose-exposure relationships.

  16. Ultra-heavy vertical lift system: The Heli-Stat. [helicopter - airship combination for materials handling

    Science.gov (United States)

    Piasecki, F. N.

    1975-01-01

    A hybrid VTOL airship which is combined with helicopters is evaluated. The static lift of the airship supports approximately the full empty weight of the entire assembly. The helicopter rotors furnish the lift to support the payload as well as the propulsion and control about all axes. Thus existing helicopters, with no new technology required, can be made to lift payloads of ten times the capacity of each one alone, and considerably more than that of any airship built so far. A vehicle is described which has a 75-ton payload, based on four existing CH-53D helicopters and an airship of 3,600,000 cu. ft. The method of interconnection is described along with discussion of control, instrumentation, drive system and critical design conditions. The vertical lift and positioning capabilities of this vehicle far exceed any other means available today, yet can be built with a minimum of risk, development cost and time.

  17. SERVING TIMES OF DISCONTINUOUS MATERIALS HANDLING MACHINES

    OpenAIRE

    PÉTER TELEK

    2014-01-01

    The performance of production systems highly depend on the effectiveness of the serving process, which consist of different materials handling operations depend on the applied machines. During the design process of serving systems there are key role of the characterisations of the handling equipment: they determine the applicability of the given machines. In my paper, I analyse the serving operations of discontinuous materials handling machines and define the time requirements of them. After ...

  18. DESIGN OF MANUAL MATERIAL HANDLING SYSTEM THROUGH COMPUTER AIDED ERGONOMICS: A CASE STUDY AT BDTSC TEXTILE FIRM

    Directory of Open Access Journals (Sweden)

    Amare Matebu

    2014-12-01

    Full Text Available Designing of lifting, pushing and pulling activities based on the physical and physiological capabilities of the operators is essential. The purpose of this study is to analyze manual material handling (MMH working posture of the operators using 3D Static Strength Prediction Program (3DSSPP software and to identify major areas causing long last injury of operators. The research has investigated the fit between the demands of tasks and the capabilities of operators. At the existing situations, the actual capabilities of operators have been computed with the help of 3DSSPP software and compared with NIOSH standards. Accordingly, operators' working posture is at an unacceptable position that exposes them for musculoskeletal disorders. Then, after the improvement of the design of MMH device (cart's roller, the result showed that the forces required by the operators to push and pull the sliver cans have been reduced from 931.77 Newton to 194.23 Newton. Furthermore, improvement of MMH cart's roller has reduced the awkward posture of operators and the risk of musculoskeletal disorders. The improved manual material handling design also saves about 1828.40 ETB per month for the company.

  19. Air-Cooled Stack Freeze Tolerance Freeze Failure Modes and Freeze Tolerance Strategies for GenDriveTM Material Handling Application Systems and Stacks Final Scientific Report

    Energy Technology Data Exchange (ETDEWEB)

    Hancock, David, W.

    2012-02-14

    Air-cooled stack technology offers the potential for a simpler system architecture (versus liquid-cooled) for applications below 4 kilowatts. The combined cooling and cathode air allows for a reduction in part count and hence a lower cost solution. However, efficient heat rejection challenges escalate as power and ambient temperature increase. For applications in ambient temperatures below freezing, the air-cooled approach has additional challenges associated with not overcooling the fuel cell stack. The focus of this project was freeze tolerance while maintaining all other stack and system requirements. Through this project, Plug Power advanced the state of the art in technology for air-cooled PEM fuel cell stacks and related GenDrive material handling application fuel cell systems. This was accomplished through a collaborative work plan to improve freeze tolerance and mitigate freeze-thaw effect failure modes within innovative material handling equipment fuel cell systems designed for use in freezer forklift applications. Freeze tolerance remains an area where additional research and understanding can help fuel cells to become commercially viable. This project evaluated both stack level and system level solutions to improve fuel cell stack freeze tolerance. At this time, the most cost effective solutions are at the system level. The freeze mitigation strategies developed over the course of this project could be used to drive fuel cell commercialization. The fuel cell system studied in this project was Plug Power's commercially available GenDrive platform providing battery replacement for equipment in the material handling industry. The fuel cell stacks were Ballard's commercially available FCvelocity 9SSL (9SSL) liquid-cooled PEM fuel cell stack and FCvelocity 1020ACS (Mk1020) air-cooled PEM fuel cell stack.

  20. Storage and Materials Handling

    Science.gov (United States)

    1979-09-01

    headquarters having stock account- 3-407. Inspection ability for the supplies. a. Spot inspection. Virtually all items of subsist- (1) If the...Platform, Gasoline, or Elec- horsepower, and traction to operate on virtually all tric types of running surfaces (fig 4-19). Application. The platform...of the fire depart- display, auto showrooms , parking garages, light ment (small hose). The system affords a ready manufacturing, warehouses not

  1. CanDan 2, phase 2. Final report. [Fuel cell systems for back-up power and materials handling applications]; CanDan 2, fase 2. Slutrapport

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2012-11-01

    CanDan 2 Phase 2 is the second phase of a research and demonstration project for fuel cell backup power systems and fuel cell powered material handling equipment. In the Backup Power segment the fuel cell units have been developed, certified and delivered. A total of 32 fuel cell backup power systems have been delivered for EnergiMidt and in operation since early 2011. Following this project EnergiMidt has purchased another 31 systems in order to make a full transition from battery backup to fuel cell backup in their entire broadband network. In the material handling segment a 10 kW fuel cell system has been fully integrated in the fork lift truck, Dantruck 3000 Power Hydrogen. The result was a much more commercially mature product than expected from the beginning of the project. The result is a finished 2,5T fork lift truck which was presented at the CE-mat fair in April 2011. (LN)

  2. Ergonomic material-handling device

    Science.gov (United States)

    Barsnick, Lance E.; Zalk, David M.; Perry, Catherine M.; Biggs, Terry; Tageson, Robert E.

    2004-08-24

    A hand-held ergonomic material-handling device capable of moving heavy objects, such as large waste containers and other large objects requiring mechanical assistance. The ergonomic material-handling device can be used with neutral postures of the back, shoulders, wrists and knees, thereby reducing potential injury to the user. The device involves two key features: 1) gives the user the ability to adjust the height of the handles of the device to ergonomically fit the needs of the user's back, wrists and shoulders; and 2) has a rounded handlebar shape, as well as the size and configuration of the handles which keep the user's wrists in a neutral posture during manipulation of the device.

  3. 48 CFR 908.7112 - Materials handling equipment replacement standards.

    Science.gov (United States)

    2010-10-01

    ... equipment replacement standards. 908.7112 Section 908.7112 Federal Acquisition Regulations System DEPARTMENT... Special Items 908.7112 Materials handling equipment replacement standards. Materials handling equipment shall be purchased for replacement purposes in accordance with the standards in FPMR 41 CFR 101-25.405...

  4. Robotic Materials Handling in Space: Mechanical Design of the Robot Operated Materials Processing System HitchHiker Experiment

    Science.gov (United States)

    Voellmer, George

    1997-01-01

    The Goddard Space Flight Center has developed the Robot Operated Materials Processing System (ROMPS) that flew aboard STS-64 in September, 1994. The ROMPS robot transported pallets containing wafers of different materials from their storage racks to a furnace for thermal processing. A system of tapered guides and compliant springs was designed to deal with the potential misalignments. The robot and all the sample pallets were locked down for launch and landing. The design of the passive lockdown system, and the interplay between it and the alignment system are presented.

  5. 2010 Manufacturing Readiness Assessment Update to the 2008 Report for Fuel Cell Stacks and Systems for the Backup Power and Materials Handling Equipment Markets

    Energy Technology Data Exchange (ETDEWEB)

    Wheeler, D.; Ulsh, M.

    2012-08-01

    In 2008, the National Renewable Energy Laboratory (NREL), under contract to the US Department of Energy (DOE), conducted a manufacturing readiness assessment (MRA) of fuel cell systems and fuel cell stacks for back-up power and material handling applications (MHE). To facilitate the MRA, manufacturing readiness levels (MRL) were defined that were based on the Technology Readiness Levels previously established by the US Department of Energy (DOE). NREL assessed the extensive existing hierarchy of MRLs developed by Department of Defense (DoD) and other Federal entities, and developed a MRL scale adapted to the needs of the Fuel Cell Technologies Program (FCTP) and to the status of the fuel cell industry. The MRL ranking of a fuel cell manufacturing facility increases as the manufacturing capability transitions from laboratory prototype development through Low Rate Initial Production to Full Rate Production. DOE can use MRLs to address the economic and institutional risks associated with a ramp-up in polymer electrolyte membrane (PEM) fuel cell production. In 2010, NREL updated this assessment, including additional manufacturers, an assessment of market developments since the original report, and a comparison of MRLs between 2008 and 2010.

  6. An Improved Dispatching Method (a-HPDB for Automated Material Handling System with Active Rolling Belt for 450 mm Wafer Fabrication

    Directory of Open Access Journals (Sweden)

    Chia-Nan Wang

    2017-07-01

    Full Text Available The semiconductor industry is facing the transition from 300 mm to 450 mm wafer fabrication. Due to the increased size and weight, 450 mm wafers will pose unprecedented challenges on semiconductor wafer fabrication. To better handle and transport 450 mm wafers, an advanced Automated Material Handling System (AMHS is definitely required. Though conveyor-based AMHS is expected to be suitable for 450 mm wafer fabrication, still it faces two main problems, traffic-jam problem and lot-prioritization. To address the two problems, in this research we have proposed an improved dispatching method, termed Heuristic Preemptive Dispatching Method using Activated Roller Belt (a-HPDB. We have developed some effective rules for the a-HPDB based on Activated Roller Belt (ARB. In addition, we have conducted experiments to investigate its effectiveness. Compared with the HPDB and R-HPD, two dispatching rules proposed in previous studies, our experimental results showed the a-HPDB had a better performance in terms of average lot delivery time (ALDT. For hot lots and normal lots, the a-HPDB had advantages of 4.14% and 8.92% over the HPDB and advantages of 4.89% and 8.52% over R-HPD, respectively.

  7. Standards for material handling and facilities equipment proofload testing

    Science.gov (United States)

    Bonn, S. P.

    1970-01-01

    Document provides information on verifying the safety of material handling and facilities equipment /MH/FE/, ranging from monorail systems to ladders and non-powered mobile equipment. Seven catagories of MH/FE equipment are defined.

  8. Safety of material handling for superconducting cyclotron

    International Nuclear Information System (INIS)

    Hajra, D.P.

    2009-01-01

    State of the art of material handling for superconducting cyclotron project has been ensured through advance safety analysis and safety planning for selection and procurement of proper material handling equipment at right time and right place to minimized time, cost over run and man-day loss. Mechanization of material handling is the most important aspect of safety. Typical problems of superconducting cyclotron material handling was solved by indigenous design, fabrication and utilization of a unique material handling equipment for 180 deg rotation of a 28 tons single weight complicated upper pole-cap assembly with safety and ease. Material handling is an art and has been implemented through the science of safe movement, handling during manufacturing and assembly and installation of SCC project. (author)

  9. 29 CFR 1926.953 - Material handling.

    Science.gov (United States)

    2010-07-01

    ... 29 Labor 8 2010-07-01 2010-07-01 false Material handling. 1926.953 Section 1926.953 Labor... Material handling. (a) Unloading. Prior to unloading steel, poles, cross arms and similar material, the... shall be attached to the trailing end of the longest pole. (c) Storage. (1) No materials or equipment...

  10. Real-time-data analytics in raw materials handling

    OpenAIRE

    Rothschedl, Christopher; Ritt, Roland; O'Leary, Paul; Harker, Matthew; Habacher, Michael; Brandner, Michael

    2018-01-01

    This paper proposes a system for the ingestion and analysis of real-time sensor and actor data of bulk materials handling plants and machinery. It references issues that concern mining sensor data in cyber physical systems (CPS) as addressed in O’Leary et al. [2015].

  11. KC-135 materials handling robotics

    Science.gov (United States)

    Workman, Gary L.

    1991-01-01

    Robot dynamics and control will become an important issue for implementing productive platforms in space. Robotic operations will become necessary for man-tended stations and for efficient performance of routine operations in a manned platform. The current constraints on the use of robotic devices in a microgravity environment appears to be due to an anticipated increase in acceleration levels due to manipulator motion and for safety concerns. The objective of this study will be to provide baseline data to meet that need. Most texts and papers dealing with the kinematics and dynamics of robots assume that the manipulator is composed of joints separated by rigid links. However, in recent years several groups have begun to study the dynamics of flexible manipulators, primarily for applying robots in space and for improving the efficiency and precision of robotic systems. Robotic systems which are being planned for implementation in space have a number of constraints to overcome. Additional concepts which have to be worked out in any robotic implementation for a space platform include teleoperation and degree of autonomous control. Some significant results in developing a robotic workcell for performing robotics research on the KC-135 aircraft in preperation for space-based robotics applications in the future were generated. In addition, it was shown that TREETOPS can be used to simulate the dynamics of robot manipulators for both space and ground-based applications.

  12. Control of automatic materials handling in radiation fields

    International Nuclear Information System (INIS)

    Szadvari, Jozsef; Juhasz, Miklos; Szanto, Robert

    1987-01-01

    Some agricultural and food industry raw materials and products can be effectively sterilized by ionizing radiation for better quality and longer shelf-life. A microelectronically controlled automatic materials handling system installed in an irradiation facility of AGROSTER Company, Hungary, is described. Programmable logic controllers (PLCs) type FESTO FPC 606 were applied to control the motion of containers. (R.P.) 6 figs

  13. Regulatory process for material handling equipment

    International Nuclear Information System (INIS)

    Rajendran, S.; Agarwal, Kailash

    2017-01-01

    Atomic Energy (Factories) Rules (AEFR) 1996, Rule 35 states, 'Thorough inspection and load testing of a Crane shall be done by a Competent Person at least once every 12 months'. To adhere to this rule, BARC Safety Council constituted 'Material Handling Equipment Committee (MHEC)' under the aegis of Conventional Fire and Safety Review Committee (CFSRC) to carry out periodical inspection and certification of Material Handling Equipment (MHE), tools and tackles used in BARC Facilities at Trombay, Tarapur and Kalpakkam

  14. Safety requirements and feedback of commonly used material handling equipment

    International Nuclear Information System (INIS)

    Pathak, M.K.

    2009-01-01

    Different types of cranes, hoists, chain pulley blocks are the most commonly used material handling equipment in industry along with attachments like chains, wire rope slings, d-shackles, etc. These equipment are used at work for transferring loads from one place to another and attachments are used for anchoring, fixing or supporting the load. Selection of the correct equipment, identification of the equipment planning of material handling operation, examination/testing of the equipment, education and training of the persons engaged in operation of the material handling equipment can reduce the risks to safety of people in workplace. Different safety systems like boom angle indicator, overload tripping device, limit switches, etc. should be available in the cranes for their safe use. Safety requirement for safe operation of material handling equipment with emphasis on different cranes and attachments particularly wire rope slings and chain slings have been brought out in this paper. An attempt has also been made to bring out common nature of deficiencies observed during regulatory inspection carried out by AERB. (author)

  15. Materials handling centre: making business more efficient

    NARCIS (Netherlands)

    B. Bollen (Brian)

    2012-01-01

    textabstractThe aim of the Materials Handling Forum at RSM is to narrow the gap between research and practice by promoting and disseminating academic knowledge, sharing innovative ideas, generating research questions, and co-developing new research themes with industry partners.

  16. 41 CFR 101-25.405 - Materials handling equipment.

    Science.gov (United States)

    2010-07-01

    ... 41 Public Contracts and Property Management 2 2010-07-01 2010-07-01 true Materials handling...-Replacement Standards § 101-25.405 Materials handling equipment. (a) Materials handling equipment will not be... materials handling equipment in storage, one month in storage equals 50 hours of operation. (ii) The number...

  17. Material Handling Equipment Selection Using Weighted Utility Additive Theory

    OpenAIRE

    Prasad Karande; Shankar Chakraborty

    2013-01-01

    Better utilization of manpower, providing product flexibility, increasing productivity, decreasing lead time, reduction in handling cost, increased efficiency of material flow, and enhancement of production process are some of the most important issues influencing material handling (MH) equipment selection decision. As a wide variety of MH equipment is available today, selection of the proper equipment for a designed manufacturing system is a complicated task. Selection of suitable MH equipme...

  18. Trial operation of material protection, control, and accountability systems at two active nuclear material handling sites within the All-Russian Institute of Experimental Physics (VNIIEF)

    International Nuclear Information System (INIS)

    Skripka, G.; Vatulin, V.; Yuferev, V.

    1997-01-01

    This paper discusses Russian Federal Nuclear Center (RFNC)-VNIIEF activities in the area of nuclear material protection, control, and accounting (MPC and A) procedures enhancement. The goal of such activities is the development of an automated systems for MPC and A at two of the active VNIIEF research sites: a research (reactor) site and a nuclear material production facility. The activities for MPC and A system enhancement at both sites are performed in the framework of a VNIIEF-Los Alamos National Laboratory contract with participation from Sandia National Laboratories, Lawrence Livermore National Laboratory, Brookhaven National Laboratory, Oak Ridge National Laboratory, Pacific Northwest National Laboratory, and PANTEX Plant in accordance with Russian programs supported by MinAtom. The American specialists took part in searching for possible improvement of technical solutions, ordering equipment, and delivering and testing the equipment that was provided by the Americans

  19. Preconceptual design of a Long-Pulse Spallation Source (LPSS) at the LANSCE Facility: Target system, facility, and material handling considerations

    International Nuclear Information System (INIS)

    Sommer, W.F.

    1995-12-01

    This report provides a summary of a preconceptual design study for the proposed Long-Pulse Spallation. Source (LPSS) at the Los Alamos Neutron Science Center (LANSCE). The LPSS will use a 0.8-MW proton beam to produce neutrons from a tungsten target. This study focuses on the design of the target station and changes to the existing building that would be made to accommodate the LPSS. The LPSS will provide fifteen flight paths to neutron scattering instruments. In addition, options for generating ultracold neutrons, pions, and muons will be available. Flight-energy, forward-scattered neutrons on the downstream side of the target will also be available for autoradiography studies. A Target Test Bed (TTB) is also proposed for full-beam tests of component materials and advanced spallation neutron sources. The design allows for separation of the experiment hall from the beam line, target, and flight paths. The target and moderator systems and the systems/components to be tested in the TTB will be emplaced and removed separately by remotely operated, shielded equipment. Irradiated materials will be transported to a hot cell adjacent to the target chamber for testing by remotely operated instruments. These tests will provide information about how materials properties are affected by proton and neutron beams

  20. Roles and responsibilities of competent person in material handling

    International Nuclear Information System (INIS)

    Pathak, M.K.

    2016-01-01

    Material handling equipment (MHE) is used for the movement and storage of material within a facility or at a site. Material handling equipment can be classified as lifting equipment, transport equipment, storage equipment etc. Out of all types of material handling equipment, lifting equipment is used widely in all types of industries. Material handling equipment which permits smooth and continuous operation, creates less accidents, reduces operation cycle time, promotes better working condition, incurs less fatigue to the operator and brings down the total material handling cost needs to be selected. Several accidents have happened in our country due to crane collapse, chain pulley block failure etc

  1. Bag-out material handling system

    Science.gov (United States)

    Brak, Stephen B.; Milek, Henry F.

    1984-01-01

    A bagging device for transferring material from a first chamber through an pening in a wall to a second chamber includes an outer housing communicating with the opening and having proximal and distal ends relative to the wall. An inner housing having proximal and distal ends corresponding to those of the outer housing is mounted in a concentrically spaced, sealed manner with respect to the distal end of the outer housing. The inner and outer housings and mounting means therebetween define an annular chamber, closed at its distal end and open at its proximal end, in which a pliable tube is slidably positioned in sealed engagement with the housings. The pliable tube includes a sealed end positioned adjacent the proximal end of the inner housing so as to maintain isolation between the first and second chambers. Displacement of the material to be bagged from the first chamber along the inner housing so as to contact the sealed portion of the pliable bag allows the material to be positioned within the pliable bag in the second chamber. The bag is then sealed and severed between where the material is positioned therein and the wall in providing a sealed container for handling the material. The pliable tube when substantially depleted slides onto a narrow portion of the inner housing to allow a new pliable tube to be positioned over the old pliable tube. Remnants of the old pliable tube are then discharged into the new pliable tube with the bagging and removal of additional material.

  2. Bag-out material handling system

    Energy Technology Data Exchange (ETDEWEB)

    Brak, S.B.

    1982-02-26

    A bagging device for transferring material from a first chamber through an opening in a wall to a second chamber includes an outer housing communicating with the opening and having proximal and distal ends relative to the wall. An inner housing having proximal and distal ends corresponding to those of the outer housing is mounted in a concentrically spaced, sealed manner with respect to the distal end of the outer housing. The inner and outer housings and mounting means therebetween define an annular chamber, closed at its distal end and open at its proximal end, in which a pliable tube is slidably positioned in sealed engagement with the housings. The pliable tube includes a sealed end positioned adjacent the proximal end of the inner housing so as to maintain isolation between the first and second chambers. Displacement of the material to be bagged from the first chamber along the inner housing so as to contact the sealed portion of the pliable bag allows the material to be positioned within the pliable bag in the second chamber. The bag is then sealed and severed between where the material is positioned therein and the wall in providing a sealed container for handling the material. The pliable tube when substantially depleted slides onto a narrow portion of the inner housing to allow a new pliable tube to be positioned over the old pliable tube. Remnants of the old pliable tube are then discharged into the new pliable tube with the bagging and removal of additional material.

  3. Design and Implementation of Company Tailored Automated Material Handling

    DEFF Research Database (Denmark)

    Langer, Gilad; Bilberg, Arne

    1996-01-01

    to implementation. The goals of the research were to analyse and evaluate automation in order to obtain an advantageous combination of human and automated resources. The idea is to asses different solutions in a virtual environment, where experiments and analyses can be performed so that the company can justify......This article focuses on the problems of analysing automation of material handling systems in order to develop an efficient automated solution that is specifically tailored to the company. The research has resulted in development of new methods for evaluating factory automation from design...

  4. The Optimal Design for Grip Force of Material Handling

    OpenAIRE

    V. Tawiwat; S. Sarawut

    2008-01-01

    Applied a mouse-s roller with a gripper to increase the efficiency for a gripper can learn to a material handling without slipping. To apply a gripper, we use the optimize principle to develop material handling by use a signal for checking a roller mouse that rotate or not. In case of the roller rotates means that the material slips. A gripper will slide to material handling until the roller will not rotate. As this experiment has test material handling for comparing a gr...

  5. Materials Handling. Module SH-01. Safety and Health.

    Science.gov (United States)

    Center for Occupational Research and Development, Inc., Waco, TX.

    This student module on materials handling is one of 50 modules concerned with job safety and health. It presents the procedures for safe materials handling. Discussed are manual handling methods (lifting and carrying by hand) and mechanical lifting (lifting by powered trucks, cranes or conveyors). Following the introduction, 15 objectives (each…

  6. 41 CFR 50-204.3 - Material handling and storage.

    Science.gov (United States)

    2010-07-01

    ... 41 Public Contracts and Property Management 1 2010-07-01 2010-07-01 true Material handling and... CONTRACTS General Safety and Health Standards § 50-204.3 Material handling and storage. (a) Where mechanical handling equipment is used, sufficient safe clearances shall be allowed for aisles, at loading docks...

  7. 29 CFR 1926.602 - Material handling equipment.

    Science.gov (United States)

    2010-07-01

    ... 29 Labor 8 2010-07-01 2010-07-01 false Material handling equipment. 1926.602 Section 1926.602... Equipment, and Marine Operations § 1926.602 Material handling equipment. (a) Earthmoving equipment; General... present no hazard to personnel from flying materials. (6) Rollover protective structures (ROPS). See...

  8. 76 FR 37118 - Manual Materials Handling (MMH) Workshop

    Science.gov (United States)

    2011-06-24

    ...: NIOSH-243] Manual Materials Handling (MMH) Workshop AGENCY: National Institute for Occupational Safety... University of Cincinnati, Department of Environmental Health, will be holding a two-day Manual Materials Handling (MMH) Workshop. The Workshop is a National Occupational Research Agenda (NORA) activity organized...

  9. Design and operation of a remotely operated plutonium waste size reduction and material handling process

    International Nuclear Information System (INIS)

    Stewart, J.A. III; Charlesworth, D.L.

    1986-01-01

    Non-combustible Pu-238 and Pu-239 waste is generated as a result of normal operation and decommissioning activity at the Savannah River Plant, and is being retrievably stored there. As part of the long-term plan to process the stored waste and current waste for permanent disposal, a remote size reduction and material handling process is being cold-tested at Savannah River Laboratory. The process consists of a large, low-speed shredder and material handling system, a remote worktable, a bagless transfer system, and a robotically controlled manipulator. Initial testing of the shredder and material handling system and a cycle test of the bagless transfer system has been completed. Fabrication and acceptance testing of the Telerobot, a robotically controlled manipulator, has been completed. Testing is scheduled to begin in 3/86. Design features maximizing the ability to remotely maintain the equipment were incorporated. Complete cold-testing of the equipment is scheduled to be completed in 987

  10. Experience in testing and inspection and maintenance of material handling equipments

    International Nuclear Information System (INIS)

    Sharma, M.L.

    2009-01-01

    All the Industries, Power Projects/Stations, Organizations engaged in the field of process of manufacturing, power generation, transportation, design, layout, manufacturing, and supply have to utilize material handling equipment, machinery tools tackles, lifting gears for performing their tasks/activities. The major role of the material handling equipments play an important role and a component of 40% of the total activities of the system/process to achieve targeted output with the reliability and quality is performed by material handling equipment and machineries. The material handling equipment shall have to be chosen/selected to suit the process requirement at times to be specifically designed inspected and tested to meet the specific requirement. These equipment/machineries/lifting gears have to undergo for the periodical inspection and testing to qualify for further use in a specified period. All those equipment and machinery to be used for material handling if not found satisfactory during inspection and testing or otherwise also shall be dismantled/stripped to the extent of inspection requirement of the components/sub components and maintenance repair shall have to be done to make them worthy for reuse after testing and inspection duly witnessed by competent authority

  11. Decision method for optimal selection of warehouse material handling strategies by production companies

    Science.gov (United States)

    Dobos, P.; Tamás, P.; Illés, B.

    2016-11-01

    Adequate establishment and operation of warehouse logistics determines the companies’ competitiveness significantly because it effects greatly the quality and the selling price of the goods that the production companies produce. In order to implement and manage an adequate warehouse system, adequate warehouse position, stock management model, warehouse technology, motivated work force committed to process improvement and material handling strategy are necessary. In practical life, companies have paid small attantion to select the warehouse strategy properly. Although it has a major influence on the production in the case of material warehouse and on smooth costumer service in the case of finished goods warehouse because this can happen with a huge loss in material handling. Due to the dynamically changing production structure, frequent reorganization of warehouse activities is needed, on what the majority of the companies react basically with no reactions. This work presents a simulation test system frames for eligible warehouse material handling strategy selection and also the decision method for selection.

  12. Fleet Sizing of Automated Material Handling Using Simulation Approach

    Science.gov (United States)

    Wibisono, Radinal; Ai, The Jin; Ratna Yuniartha, Deny

    2018-03-01

    Automated material handling tends to be chosen rather than using human power in material handling activity for production floor in manufacturing company. One critical issue in implementing automated material handling is designing phase to ensure that material handling activity more efficient in term of cost spending. Fleet sizing become one of the topic in designing phase. In this research, simulation approach is being used to solve fleet sizing problem in flow shop production to ensure optimum situation. Optimum situation in this research means minimum flow time and maximum capacity in production floor. Simulation approach is being used because flow shop can be modelled into queuing network and inter-arrival time is not following exponential distribution. Therefore, contribution of this research is solving fleet sizing problem with multi objectives in flow shop production using simulation approach with ARENA Software

  13. Experiences in safe use of material handling equipment at BARC

    International Nuclear Information System (INIS)

    Bharambe, S.D.; Padmavathi, G.L.N.; Soundararajan, S.

    2009-01-01

    Material handling is a basic requirement of all the tasks we perform. Equipment used for handling of materials provide great flexibility in relieving man-power, reduced time and in ensuring safety of men and materials. These Materials Handling Equipment (MHE) refer to various materials handling equipment, including forklifts, cranes, chain pulley blocks, tractors, and other specialized industrial trucks powered by electric motors or internal combustion engines. Proper maintenance of these equipment is essential, because, in addition to enhanced safety, it prevents loss of business or production caused by mechanical failure. This paper discusses on the experiences in respect of safe use of MHE achieved through a typical systematic approach to maintenance of material handling equipment. (author)

  14. Challenges, difficulties and safe work practices in material handling

    International Nuclear Information System (INIS)

    Singh, Rajvir

    2009-01-01

    Material handling (MH) is an essential part of erection, installation, loading and unloading of heavy machineries and equipments in any plant, factory or an organization. It makes difficult jobs easy and it enhances safe working atmosphere of the plant/unit. Material handling methods are required to be performed as per standard practices and with proper equipments to avoid losses of resources and fatal accidents. Any ill-practice will make simple tasks of material handling more difficult and time-costing. There are several causes which make the material handling more challenging and difficult. Difficult-to-Perform MH assignments of the units can be accomplished in a simple and easy to handle manner by paying attention to the factors as mentioned in this paper. (author)

  15. Analysis of Material Handling Safety in Construction Sites and Countermeasures for Effective Enhancement.

    Science.gov (United States)

    Kumar, C N Anil; Sakthivel, M; Elangovan, R K; Arularasu, M

    2015-01-01

    One of many hazardous workplaces includes the construction sites as they involve several dangerous tasks. Many studies have revealed that material handling equipment is a major cause of accidents at these sites. Though safety measures are being followed and monitored continuously, accident rates are still high as either workers are unaware of hazards or the safety regulations are not being strictly followed. This paper analyses the safety management systems at construction sites through means of questionnaire surveys with employees, specifically referring to safety of material handling equipment. Based on results of the questionnaire surveys, two construction sites were selected for a safety education program targeting worker safety related to material handling equipment. Knowledge levels of the workers were gathered before and after the program and results obtained were subjected to a t-test analysis to mark significance level of the conducted safety education program.

  16. Analysis of Material Handling Safety in Construction Sites and Countermeasures for Effective Enhancement

    Directory of Open Access Journals (Sweden)

    C. N. Anil Kumar

    2015-01-01

    Full Text Available One of many hazardous workplaces includes the construction sites as they involve several dangerous tasks. Many studies have revealed that material handling equipment is a major cause of accidents at these sites. Though safety measures are being followed and monitored continuously, accident rates are still high as either workers are unaware of hazards or the safety regulations are not being strictly followed. This paper analyses the safety management systems at construction sites through means of questionnaire surveys with employees, specifically referring to safety of material handling equipment. Based on results of the questionnaire surveys, two construction sites were selected for a safety education program targeting worker safety related to material handling equipment. Knowledge levels of the workers were gathered before and after the program and results obtained were subjected to a t-test analysis to mark significance level of the conducted safety education program.

  17. ASRS application in radioactive material handling

    International Nuclear Information System (INIS)

    Shalina Sheik Muhamad and Rosli Darmawan

    2007-01-01

    ASRS is a computer controlled methods for automatically depositing and retrieving loads from defined storage locations. It consists of several major components which are the Controls System, Storage Structure (Racks), Storage/Retrieval (S/R) Machines, Fire Protection Systems, Pickup and Delivery (P and D) Stations, Storage Modules (Pallets, Baskets, Containers), Aisle Hardware, Aisle Transfer Cars, Conveyors and other transportation equipment. This paper aims to provide an overview of the potential application of the ASRS in Nuclear Malaysia. The need to use the system, operating principle and potential application will be discussed. (Author)

  18. Modelling of material handling operations using controlled traffic

    DEFF Research Database (Denmark)

    Bochtis, Dionysis; Sørensen, Claus Aage Grøn; Jørgensen, Rasmus Nyholm

    2009-01-01

    and maintaining permanent traffic lanes within the fields. Furthermore, field efficiency is affected by CTF due to significant increases in idle time of in-field transport and the way the fields are traversed in material handling operations. During fertilisation, when tramline length and the driving distance......, makes existing models inadequate for evaluating field efficiency. In this paper, the development of a discrete-event model for the prediction of travelled distances of a machine operating in material handling operations using the concept of CTF is presented. The model is based on the mathematical...

  19. An Analysis of the Terminal Materials Handling Occupation.

    Science.gov (United States)

    Mascio, Joseph W.; O'Connor, Patrick J.

    The general purpose of the occupational analysis is to provide workable, basic information dealing with the many and varied duties performed in the terminal materials handling occupation. The document opens with a brief introduction followed by a job description. The bulk of the document is presented in table form. Five duties are broken down into…

  20. An investigation of posture and manual materials handling as risk ...

    African Journals Online (AJOL)

    The prevalence of low back pain symptoms, posture and manual materials handling among delivery drivers was investigated. Information about health history, driving experience, work postures and MMH was obtained from 49 male and 2 female persons who did intra-city deliveries and drove vehicles such as trucks, vans ...

  1. The Status of Energy Monitoring in Science and Industry by the Example of Material Handling Processes

    OpenAIRE

    Patrick Fekete; Steve Martin; Katja Kuhn; Nicholas Wright

    2014-01-01

    Global mega trends attract increased resource preservation as well as system efficiency and arouse growing scientific, industrial and public attention, whereas process and technologic developments still lack realisation due to inaccurate knowledge of real process energy demand, associated possible savings and a low inducement for investment. This investigation develops a generally applicable process for energy data collection focussing on material handling systems based on a predefined target...

  2. Eco Issues in Bulk Materials Handling Technologies in Ports

    OpenAIRE

    Nenad Zrnic; Milos Dordevic; Branislav Dragovic; Srdan Bosnjak

    2011-01-01

    This paper deals with eco issues in bulk materials handling in ports. Solid, free-flowing materials are said to be in bulk. Bulk materials han-dling is very difficult, because it incorporates all the features of liquids, gasses and mass solids. Energy efficiency, dust emissions in nearby en-vironment, dust explosions, jamming, noise, handling of hazardous ma-terials and protection of materials from contamination are issues that will be considered in this paper. Here are also presented possibl...

  3. Product Manager Combat Engineering and Material Handling Equipment

    Science.gov (United States)

    2012-04-16

    Asphalt Mixing Plant  Skid Steer Loader Type 2 & 3  EMM Water Distributor  Hasty Road Repair  Soil Density Tester  Vibratory Plate Compactor...Engineer/Material Handling Equipment  Eng Rapid Airfield Cont Capability IV  Bituminous Paving Machine  Type 2 Heavy ATEC  Water Well Drill Rig...maneuver support, enable theater access, assured mobility, and airstrip/airfield construction . The FOD also provides earthmoving operational capabilities

  4. Analysis of Material Handling Safety in Construction Sites and Countermeasures for Effective Enhancement

    OpenAIRE

    Anil Kumar, C. N.; Sakthivel, M.; Elangovan, R. K.; Arularasu, M.

    2015-01-01

    One of many hazardous workplaces includes the construction sites as they involve several dangerous tasks. Many studies have revealed that material handling equipment is a major cause of accidents at these sites. Though safety measures are being followed and monitored continuously, accident rates are still high as either workers are unaware of hazards or the safety regulations are not being strictly followed. This paper analyses the safety management systems at construction sites through means...

  5. REMOTE MATERIAL HANDLING IN THE YUCCA MOUNTAIN WASTE PACKAGE CLOSURE CELL AND SUPPORT AREA GLOVEBOX

    International Nuclear Information System (INIS)

    K.M. Croft; S.M. Allen; M.W. Borland

    2005-01-01

    The Yucca Mountain Waste Package Closure System (WPCS) cells provide for shielding of highly radioactive materials contained in unsealed waste packages. The purpose of the cells is to provide safe environments for package handling and sealing operations. Once sealed, the packages are placed in the Yucca Mountain Repository. Closure of a typical waste package involves a number of remote operations. Those involved typically include the placement of matched lids onto the waste package. The lids are then individually sealed to the waste package by welding. Currently, the waste package includes three lids. One lid is placed before movement of the waste package to the closure cell; the final two are placed inside the closure cell, where they are welded to the waste package. These and other important operations require considerable remote material handling within the cell environment. This paper discusses the remote material handling equipment, designs, functions, operations, and maintenance, relative to waste package closure

  6. Remote automated material handling of radioactive waste containers

    International Nuclear Information System (INIS)

    Greager, T.M.

    1994-09-01

    To enhance personnel safety, improve productivity, and reduce costs, the design team incorporated a remote, automated stacker/retriever, automatic inspection, and automated guidance vehicle for material handling at the Enhanced Radioactive and Mixed Waste Storage Facility - Phase V (Phase V Storage Facility) on the Hanford Site in south-central Washington State. The Phase V Storage Facility, scheduled to begin operation in mid-1997, is the first low-cost facility of its kind to use this technology for handling drums. Since 1970, the Hanford Site's suspect transuranic (TRU) wastes and, more recently, mixed wastes (both low-level and TRU) have been accumulating in storage awaiting treatment and disposal. Currently, the Hanford Site is only capable of onsite disposal of radioactive low-level waste (LLW). Nonradioactive hazardous wastes must be shipped off site for treatment. The Waste Receiving and Processing (WRAP) facilities will provide the primary treatment capability for solid-waste storage at the Hanford Site. The Phase V Storage Facility, which accommodates 27,000 drum equivalents of contact-handled waste, will provide the following critical functions for the efficient operation of the WRAP facilities: (1) Shipping/Receiving; (2) Head Space Gas Sampling; (3) Inventory Control; (4) Storage; (5) Automated/Manual Material Handling

  7. Investigation into slipping and falling accidents and materials handling in the South African mining industry.

    CSIR Research Space (South Africa)

    Schutte, PC

    2003-03-01

    Full Text Available The objective of this study was to analyze information on slipping and falling accidents and materials handling activities in the South African mining industry. Accident data pertaining to slipping, falling and materials handling accidents...

  8. 29 CFR 1926.1000 - Rollover protective structures (ROPS) for material handling equipment.

    Science.gov (United States)

    2010-07-01

    ... 29 Labor 8 2010-07-01 2010-07-01 false Rollover protective structures (ROPS) for material handling... (ROPS) for material handling equipment. (a) Coverage. (1) This section applies to the following types of material handling equipment: To all rubber-tired, self-propelled scrapers, rubber-tired front-end loaders...

  9. Manual material handling assessment among workers of Iranian casting workshops.

    Science.gov (United States)

    Mohammadi, Heidar; Motamedzade, Majid; Faghih, Mohammad Amin; Bayat, Hadi; Mohraz, Majid Habibi; Musavi, Saeed

    2013-01-01

    Manual material handling (MMH) tasks can be found in most workplaces and they may constitute a risk factor for work-related musculoskeletal disorders (WMSDs). This study was conducted to determine the prevalence of WMSDs and to compare MMH loads with the acceptable weight and force limits among Iranian casting workers. Data were collected from 50 workers of casting workshops who performed MMH tasks. The Nordic musculoskeletal disorders questionnaire and the Snook tables were used as tools for data collection. Hand/wrist symptoms were the most prevalent problems among the workers (84%). The results of the Snook tables showed that the loads in lifting (84%), lowering (86%), carrying (66%), pushing with initial (43%) and sustained force (59%), and pulling tasks with initial (48%) and sustained force (93%) exceeded recommended limits. WMSDs occurred in high rates among the workers and, thus, ergonomics interventions should focus on decreasing WMSDs and redesigning MMH tasks.

  10. Method of preventing contaminations in radioactive material handling facilities

    International Nuclear Information System (INIS)

    Inoue, Shunji.

    1986-01-01

    Purpose: To prevent the contamination on the floor surface of working places by laying polyvinyl butyral sheets over the floor surface, replacing when the sheets are contaminated, followed by burning. Method: Polyvinyl butyral sheets comprising 50 - 70 mol% of butyral component are laid in a radioactive material handling facility, radioactive materials are handled on the polyvinyl butyral sheets and the sheets are replaced when contaminated. The polyvinyl butyral sheets used contain 62 - 68 mol% of butyral component and has 0.03 - 0.2 mm thickness. The contaminated sheets are subjected to burning processing. This can surely collect radioactive materials and the sheets have favorable burnability, releasing no corrosive or deleterious gases. In addition, they are inexpensive and give no hindrance to the workers walking. (Takahashi, M.)

  11. Spinal loading during manual materials handling in a kneeling posture.

    Science.gov (United States)

    Splittstoesser, Riley E; Yang, Gang; Knapik, Greg G; Trippany, David R; Hoyle, Jeff A; Lahoti, Parul; Korkmaz, Sahika Vatan; Sommerich, Carolyn M; Lavender, Steven A; Marras, William S

    2007-02-01

    Stooped, restricted, kneeling, and other awkward postures adopted during manual materials handling have frequently been associated with LBP onset. However, lift assessment tools have focused on materials handling performed in an upright, or nearly upright standing posture. Unfortunately, many of the tools designed to analyze standing postures are not easily adapted to jobs requiring restricted postures. Therefore, the objective of this study was to evaluate spinal loading during manual materials handing in kneeling postures and determine if those loads can be predicted using simple regression. An EMG-driven biomechanical model, previously validated for upright lifting, was adapted for use in kneeling tasks. Subjects knelt under a 1.07m ceiling and lifted luggage of six weights (6.8, 10.9, 15.0, 19.1, 23.1 and, 27.2kgf) to one of four destination heights (0, 25.4, 53.3, 78.7cm). Spine loading was significantly affected by both destination height and load weight. Destination height increased compression, AP shear and lateral shear by an average of 14.5, 3.7 and 6.6N respectively per cm height increase. Load weight increased compression, AP shear and lateral shear by an average of 83.8, 27.0 and 13.1N respectively per kgf lifted. Regression equations were developed to predict peak spine loading using subject height, load weight and destination height with R(2) values of 0.62, 0.51 and 0.57 for compression, AP and lateral shear respectively.

  12. Production planning and scheduling with material handling using modelling and simulation

    Directory of Open Access Journals (Sweden)

    Krenczyk Damian

    2017-01-01

    Full Text Available Increase flexibility of manufacturing requires implementation of effective planning IT tools. Planning and scheduling problems traditionally consider the production resources as the only constraints. Since the raw material and intermediate transferring times are relevant in relation to the production times, it is required to model the material handling equipment and its activities. The problem of scheduling subject to manufacturing and logistical constraints based on data-driven models generation method is presented. A KbRS and FlexSim systems are used as a scheduling tool.

  13. Reconfigurable materials handling system incorporating part tracking, routing and scheduling

    CSIR Research Space (South Africa)

    Naidu, P

    2006-07-01

    Full Text Available . In this regard the tracking of the parts within the manufacturing cycle is important for ascertaining the location of parts within the cycle as well as the history of the completed parts. This paper outlines the development of a reconfigurable manufacturing...

  14. Configuring a robotic system for nuclear materials handling applications

    International Nuclear Information System (INIS)

    Costa, J.; d'Arbeloff, N.

    1987-01-01

    At Los Alamos National Laboratory, a Precision Robot, Inc. robot is used to manipulate radioactive samples (powder oxides) within a hot box environment. The application involves the manipulation of stainless steel sample tubes (capsules), a bulk oxide container, and small sample vials. During the actual application, welded tubes are placed into an automatic capsule cutter for opening. The robot then retrieves these tubes from the cutting input station and places the tube into a feeding device, which pours the powder into a tared container on a balance. After the net weight of the oxide has been obtained, the container is then removed from the scale by the robot and given back to the feeder mechanism. The oxides are then measured into small vials, each containing 1-g lots. These vials are then transferred by the robot into a rack, after which the entire rack is transferred to an output station at the far end of the hot box

  15. Energy-saving methodology for material handling applications

    Energy Technology Data Exchange (ETDEWEB)

    Makris, P.A.; Makri, A.P.; Provatidis, C.G. [National Technical University of Athens, School of Mechanical Engineering, Mechanical Design and Control Systems Division, 9 Iroon Polytechniou Street, Zografou Campus, GR-15773 Athens (Greece)

    2006-10-15

    This paper presents an energy saving approach to the problem of order picking in warehousing environment, which is directly related to the well-known Traveling Salesman Problem (TSP). While the available heuristic algorithms for the order-picking problem search for the route that minimizes the travel time, here the problem is addressed from the energy saving point of view. In a few words, the least energy-consuming route is identified in order to quantify the trade off in time and energy between the fastest route and the most energy economic one. Keeping in mind that often energy is as important as time, especially during a low-demand period, the current paper sheds some light into a two dimensional way of addressing the warehouse material handling problem, which saves time as well as energy. A very interesting finding is that a relatively small loss of service time in many cases may lead to a significant decrease of consumed energy without any additional cost. (author)

  16. Materials handling ability of regular and reserve British Army soldiers.

    Science.gov (United States)

    Williams, Alun G; Evans, Paul

    2007-02-01

    The main objective of this research was to compare the materials handling ability, aerobic power, and body composition of fully trained Reserve and Regular male British Army soldiers (approximately 6-7 years of military experience). Twenty-one Reserve soldiers and 15 Regular soldiers completed fitness tests, including a maximal box lift to 1.45 m and a repetitive 22-kg box lift-and-carry test. There were no statistically significant differences (p > 0.05) between the two groups in any parameter measured. The performance levels observed in these fully trained soldiers (maximal box lift approximately 63 kg, repetitive lift and carry approximately 34 repetitions) were broadly comparable to those reported previously for new recruits. The physical training performed by the Regular soldiers (both within and beyond their military service) and the Reserve soldiers (predominantly outside their military duties) seems to be of sufficient volume and intensity to maintain performance levels comparable with each other and equivalent new army recruits.

  17. Assessing materials handling and storage capacities in port terminals

    Science.gov (United States)

    Dinu, O.; Roşca, E.; Popa, M.; Roşca, M. A.; Rusca, A.

    2017-08-01

    Terminals constitute the factual interface between different modes and, as a result, buffer stocks are unavoidable whenever transport flows with different discontinuities meet. This is the reason why assessing materials handling and storage capacities is an important issue in the course of attempting to increase operative planning of logistic processes in terminals. Proposed paper starts with a brief review of the compatibilities between different sorts of materials and corresponding transport modes and after, a literature overview of the studies related to ports terminals and their specialization is made. As a methodology, discrete event simulation stands as a feasible technique for assessing handling and storage capacities at the terminal, taking into consideration the multi-flows interaction and the non-uniform arrivals of vessels and inland vehicles. In this context, a simulation model, that integrates the activities of an inland water terminal and describes the essential interactions between the subsystems which influence the terminal capacity, is developed. Different scenarios are simulated for diverse sorts of materials, leading to bottlenecks identification, performance indicators such as average storage occupancy rate, average dwell or transit times estimations, and their evolution is analysed in order to improve the transfer operations in the logistic process

  18. Evaluation of the Total Cost of Ownership of Fuel Cell-Powered Material Handling Equipment

    Energy Technology Data Exchange (ETDEWEB)

    Ramsden, T.

    2013-04-01

    This report discusses an analysis of the total cost of ownership of fuel cell-powered and traditional battery-powered material handling equipment (MHE, or more typically 'forklifts'). A number of fuel cell MHE deployments have received funding support from the federal government. Using data from these government co-funded deployments, DOE's National Renewable Energy Laboratory (NREL) has been evaluating the performance of fuel cells in material handling applications. NREL has assessed the total cost of ownership of fuel cell MHE and compared it to the cost of ownership of traditional battery-powered MHE. As part of its cost of ownership assessment, NREL looked at a range of costs associated with MHE operation, including the capital costs of battery and fuel cell systems, the cost of supporting infrastructure, maintenance costs, warehouse space costs, and labor costs. Considering all these costs, NREL found that fuel cell MHE can have a lower overall cost of ownership than comparable battery-powered MHE.

  19. MHAC--an assessment tool for analysing manual material handling tasks.

    Science.gov (United States)

    Batish, Ajay; Singh, Tejinder P

    2008-01-01

    This paper describes an assessment tool for analysing material handling tasks and its application for material handling tasks prevalent in engine bearing industry. After a close observation of material handling tasks spread over many days, a list of tasks and parameters/variables affecting those tasks was made. Ergonomic conditions present in these tasks and their deficiencies were then identified and on the basis of the relationships between the tasks and their affinities, categories were developed. Using the data of those categories and various conditions and parameters, an assessment tool called MHAC (material handling assessment chart) was developed.

  20. 41 CFR 102-42.75 - How are gifts containing hazardous materials handled?

    Science.gov (United States)

    2010-07-01

    ... hazardous materials handled? 102-42.75 Section 102-42.75 Public Contracts and Property Management Federal... § 102-42.75 How are gifts containing hazardous materials handled? Gifts containing hazardous materials are handled in accordance with the requirements and provisions of this part and part 101-42 of this...

  1. 77 FR 23117 - Rigging Equipment for Material Handling Construction Standard; Correction and Technical Amendment

    Science.gov (United States)

    2012-04-18

    ... Equipment for Material Handling Construction Standard; Correction and Technical Amendment AGENCY... AND HEALTH REGULATIONS FOR CONSTRUCTION Subpart H--Materials Handling, Storage, Use, and Disposal 0 1... amendment. SUMMARY: OSHA is correcting its sling standard for construction titled ``Rigging Equipment for...

  2. The Status of Energy Monitoring in Science and Industry by the Example of Material Handling Processes

    Directory of Open Access Journals (Sweden)

    Patrick Fekete

    2014-06-01

    Full Text Available Global mega trends attract increased resource preservation as well as system efficiency and arouse growing scientific, industrial and public attention, whereas process and technologic developments still lack realisation due to inaccurate knowledge of real process energy demand, associated possible savings and a low inducement for investment. This investigation develops a generally applicable process for energy data collection focussing on material handling systems based on a predefined target model for energy monitoring in order to generate a valid reference model. The introduction of Standardised Energy Consuming Activities (SECA model enables the development of energy based process functions as reference for its implementation to energetic investigations in various industrial applications. Analysing current and target state of energy monitoring in scientific and industrial investigations for logistics shows the developmental deficit of standardisation and realisation in energy monitoring.

  3. MICRO-MATERIAL HANDLING, EMPLOYING E-BEAM COATINGS OF COPPER AND SILVER

    Directory of Open Access Journals (Sweden)

    S. Matope

    2012-01-01

    Full Text Available

    ENGLISH ABSTRACT: Van der Waals forces and other adhesive forces impose great challenges on micro-material handling. Mechanical grippers fail to release micro-parts reliably because of them. This paper explores how the problematic Van der Waals forces may be used for micro-material handling purposes using surface roughnesses generated by e-beam coatings of copper and silver on silicon. An atomic force microscope, model Asylum MFP 3 D-Bio with version 6.22A software, was used to measure the forces exerted by the surfaces. A silver coating of 1.41 nm rms surface roughness value is found to exert the highest Van der Waals force, followed by a copper coating of 2.72 nm rms; a copper coating of 217 nm rms exerts the least force. This implies that, in a reliable micro-material handling system, these coatings are suitable for the interactive surfaces of the placement position, micro-gripper, and the pick-up position respectively.

    AFRIKAANSE OPSOMMING: Van der Waalskragte en ander bindingskragte hou steeds groot uitdagings in vir mikromateriaalhantering. As gevolg van hierdie bindingskragte stel meganiese gryptoerusting nie die mikro-partikels vry nie. Hierdie artikel ondersoek hoe die Van der Waalskragte gebruik kan word vir die mikro-materiaalhanteringsproses deur die gebruik van oppervlakgrofheid gegenereer deur ’n e-straal-laagbedekking van koper en silwer op silikon. ’n Atoomkrag mikroskoop, model Asylum MFP 3 D-Bio met weergawe 6.22A programmatuur, is gebruik om die kragte deur die oppervlakke uitgeoefen te meet. Daar is gevind dat ’n silwer laagbedekking met ’n oppervlakgrofheid van 1.41nm wortel-gemiddelde-kwadraat (wgk die hoogste Van der Waalskrag uitoefen, gevolg deur ’n koper laagbedekking met ’n oppervlakgrofheid van 2.72nm wgk; ’n koper laagbedekking met ’n grofheid van 217nm wgk het die kleinste krag uitgeoefen. Dit impliseer dat, vir ’n betroubare mikro-materiaalhanteringsisteem, hierdie laagbedekkings geskik

  4. Teknik Squat dan Stoop Menggunakan Electromyography pada Pekerjaan Manual Materials Handling

    OpenAIRE

    I Wayan Surata

    2013-01-01

    Manual materials handling has been identified as the most common cause of work-related musculoskeletal disorders. Among the manual material handling activities, lifting has long been regarded as an activity associated with a high risk of low-back pain. Lifting studies have mainly focused on the squat and stoop lifting techniques as an effort to improve the protection of workers from low back discomfort. However, neither is ideal and the benefits of one technique over another have proved incon...

  5. Comparison of new multi-criteria decision making methods for material handling equipment selection

    OpenAIRE

    Manoj Mathew; Sagar Sahu

    2018-01-01

    Conveyors and automated guided vehicles (AGVs) are important mechanical handling equipment used to transport loads from one place to another. Selection of proper material handling equipment is an intricate process. In the present paper two material handling equipment selection problem was solved using various newly developed multi criteria decision making (MCDM) methods. First a conveyor selection problem with six conflicting criteria and four alternatives was solved using four MCDM methods, ...

  6. 30 CFR 250.108 - What requirements must I follow for cranes and other material-handling equipment?

    Science.gov (United States)

    2010-07-01

    ... other material-handling equipment? 250.108 Section 250.108 Mineral Resources MINERALS MANAGEMENT SERVICE... Performance Standards § 250.108 What requirements must I follow for cranes and other material-handling... platform. (f) You must operate and maintain all other material-handling equipment in a manner that ensures...

  7. 29 CFR 1917.50 - Certification of marine terminal material handling devices (See also mandatory appendix I, of...

    Science.gov (United States)

    2010-07-01

    ... 29 Labor 7 2010-07-01 2010-07-01 false Certification of marine terminal material handling devices... TERMINALS Cargo Handling Gear and Equipment § 1917.50 Certification of marine terminal material handling... Secretary. (c) The marine terminal material handling devices listed below shall be certificated in the...

  8. 75 FR 52033 - Rigging Equipment for Material Handling; Extension of the Office of Management and Budget's (OMB...

    Science.gov (United States)

    2010-08-24

    ...] Rigging Equipment for Material Handling; Extension of the Office of Management and Budget's (OMB) Approval...), and (iii) and (f)(2) of the Standard on Rigging Equipment for Material Handling (29 CFR 1926.251... contained in the Standard on Rigging Equipment for Material Handling (29 CFR 1926.251). The Agency is...

  9. 78 FR 70326 - Rigging Equipment for Material Handling; Extension of the Office of Management and Budget's (OMB...

    Science.gov (United States)

    2013-11-25

    ...] Rigging Equipment for Material Handling; Extension of the Office of Management and Budget's (OMB) Approval... on Rigging Equipment for Material Handling (29 CFR 1926.251). These paragraphs require affixing... the Standard on Rigging Equipment for Material Handling (29 CFR 1926.251). The Agency is requesting an...

  10. Topics in programmable automation. [for materials handling, inspection, and assembly

    Science.gov (United States)

    Rosen, C. A.

    1975-01-01

    Topics explored in the development of integrated programmable automation systems include: numerically controlled and computer controlled machining; machine intelligence and the emulation of human-like capabilities; large scale semiconductor integration technology applications; and sensor technology for asynchronous local computation without burdening the executive minicomputer which controls the whole system. The role and development of training aids, and the potential application of these aids to augmented teleoperator systems are discussed.

  11. Regulatory inspection practices for industrial safety (electrical, mechanical, material handling and conventional aspects)

    International Nuclear Information System (INIS)

    Agarwal, K.

    2017-01-01

    Regulatory Inspection (RI) of BARC facilities and projects are carried out under the guidance of BARC Safety Council (BSC) Secretariat. Basically facilities and projects have been divided into two board categories viz. radiological facilities and non-radiological facilities. The Rls of radiological facilities should be carried out under OPSRC and of non-radiological facilities under CFSRC. Periodicity of inspection shall be at least once in a year. The RI of projects is carried out under concerned DSRC. RI practices with industrial safety which includes electrical, mechanical, material handling and conventional aspect for these facilities starts with check lists. The inspection areas are prepared in the form of checklists which includes availability of approved documents, compliance status of previous RIT and various safety committee's recommendations, radiological status of facilities, prompt reporting of safety related unusual occurrences, major incident, site visit for verification of actual status of system/plant. The practices for inspection in the area of electrical safety shall include checking of maintenance procedure for all critical class IV system equipment's such as HT panel, LT panel, transformer and motors. Load testing of Class III system such as D.G. set etc. shall be carried out as technical specification surveillance schedule. Status of aviation lights, number of qualified staff, availability of qualified staff etc. shall be form of inspection

  12. Space Station Freedom Toxic and Reactive Materials Handling

    Science.gov (United States)

    Baugher, Charles R. (Editor)

    1990-01-01

    Viable research in materials processing in space requires the utilization of a wide variety of chemicals and materials, many of which are considered toxic and/or highly reactive with other substances. A realistic view of the experiments which are most likely to be accomplished in the early Space Station phases are examined and design issues addressed which are related to their safe implementation. Included are discussions of materials research on Skylab, Spacelab, and the Shuttle mid-deck; overviews of early concepts for specialized Space Station systems designed to help contain potential problems; descriptions of industrial experience with ground-based research; and an overview of the state-of-the-art in contamination detection systems.

  13. Lifting Posture Analysis in Material Handling Using Virtual Humans

    Science.gov (United States)

    2005-11-01

    virtual work done by (non-inertial) forces and torques. The virtual work is the sum of the virtual works done by conservative forces and non...the system, W nc is the virtual work done by non-conservative forces and torques, and L T V= − is called the Lagrangian function. Then equation (8...Then the variation of virtual work done by non- conservative forces is T T nc W δ δ δ δ δ δ δ     = + + = +         x F q τ

  14. MICRO-MATERIAL HANDLING EMPLOYING E-BEAM GENERATED TOPOGRAPHIES OF COPPER AND ALUMINIUM

    Directory of Open Access Journals (Sweden)

    S. Matope

    2012-01-01

    Full Text Available

    ENGLISH ABSTRACT: This paper focuses on the employment of copper and aluminium in a micro-material handling system actuated by Van der Waals forces. Electron beam (e-beam evaporator deposited both materials on a silicon substrate at a rate of 0.6-1.2 Angstroms/second, vacuum pressure between 2x10-6 and 3x10-6mbar, and at a current less than 10mA. A Veeco NanoMan V Atomic Force Microscope with Nanoscope version 7.3 software was used to analyse the root mean square (rms surface roughnesses of the generated topographies. Rumpf-Rabinovich’s rms formula was used to determine the Van der Waals forces exerted by the surfaces. It was synthesised that an e-beam deposition of 7 minutes’ duration on both materials produced an optimum micro-material handling solution, with copper suitable for the pick-up position and aluminium for the placement position.

    AFRIKAANSE OPSOMMING: Die fokus van die artikel is op die gebruik van koper en aluminium in ‘n mikromateriaalhanteringstelsel, aangedryf deur Van der Waalskragte. ‘n Elektronstraal-verdamper plaas albei materiale op ‘n silikonbasis teen ‘n tempo van 0.6-1.2 Angstrom/sekonde, vakuumdruk tussen 2x10-6 en 3x10-6mbar, en teen ‘n stroom van minder as 10mA. ‘n Veeco NanoMan V Atomic Force mikroskoop, met Nanoscope 7.3 program-matuur is gebruik om die wortel-gemiddelde-kwadraat (wgk oppervlak ruheid van die gegenereerde topografieë te analiseer. Rumpf-Rabinovich se wgk-formule is gebruik om die Van der Waalskrage wat deur die oppervlaktes uitgeoefen word te bepaal. Dit is vasgestel dat ‘n elektronstraalafsetting van 7 minute op albei materiale die optimale materiaalhanteringoplossing bied, met koper geskik vir die optelposisie en aluminium vir die plasingsposisie.

  15. Evaluation and selection of material handling equipment in iron and steel industry using analytic hierarchy process

    Science.gov (United States)

    Varun, Sajja; Harshita, Raj; Pramod, Sesha; Nagaraju, Dega

    2017-05-01

    The paper presents the analytic hierarchy process (AHP) as a potential decision making method for use in the selection of the most suitable material handling (MH) system in an iron and steel industry. In this study, AHP is used in assessing the various material transportation systems employed in a steel manufacturing industry and to decide the best equipment to be used. Information on the use of AHP in evaluating MH equipment is provided and an AHP model is proposed to guide the management of an iron and steel Industry, i.e., JSW Steel Ltd. Most important factors while selecting material transportation equipment and their relative influence on the objective of decision-making model are found. A total of seven decision criteria and five different alternatives are considered for this purpose. Each alternative is evaluated in terms of the decision criteria and the relative importance (or weight) of each criterion is estimated. From the obtained pairwise comparison matrices, the best alternative is chosen. This paper provides a good insight into a decision-making model to guide managers for assessing the various material transportation equipment that are commonly employed in a steel manufacturing plant.

  16. PEMBENTUKAN SEL-SEL MESIN UNTUK MENDAPATKAN PENGURANGAN JARAK DAN BIAYA MATERIAL HANDLING DENGAN METODE HEURISTIK DI PT. BENGKEL COKRO BERSAUDARA

    Directory of Open Access Journals (Sweden)

    Bambang Purwanggono

    2012-02-01

    Full Text Available Tata letak pabrik dapat didefinisikan sebagai tata cara pengaturan fasilitas-fasilitas pabrik dengan memanfaatkan luas seoptimal mungkin guna menunjang kelancaran proses produksi. Tata letak fasilitas pada PT. Cokro Bersaudara diatur berdasarkan process layout dimana segala jenis mesin / fasilitas produksi lainnya yang memiliki tipe atau jenis yang sama ditempatkan dalam satu tempat. Dengan layout seperti itu perusahaan memperoleh keuntungan berupa fleksibilitas dalam memproduksi produk yang memiliki tingkat variasi yang tinggi, namun sebagai akibatnya perusahaan menghadapi permasalahan berupa tingginya kebutuhan material handling. Cellular Manufacturing System adalah aplikasi dari Group Technology yang merupakan metode pengaturan fasilitas-fasilitas produksi yang dibutuhkan untuk memproses suatu part family tertentu kedalam sel manufaktur. Dengan menerapkan Cellular Manufacturing System dapat diketahui pengurangan jarak antar mesin dan biaya material handling. Berdasarkan pengolahan data menggunakan algoritma heuristik yaitu Bond Energy Algorithm (BEA, Rank Order Clustering (ROC, dan Rank Order Clustering 2 (ROC 2 disimpulkan bahwa metode terpilih adalah metode BEA, dengan mengelompokkan 6 mesin (M dan 6 komponen (P kedalam 2 sel manufaktur, dimana sel 1 (M4, M6, M1, M2, P2, P5, P6, P1 dan sel 2 (M4, M6, M1, M3, M5, P3, P4. Dengan perubahan layout ini didapatkan pengurangan total jarak material handling sebesar 428,06 meter dan pengurangan biaya material handling sebesar Rp. 2.111.316,058 / bulan Kata Kunci : Cellular Manufacturing System, Algoritma Heuristik, Gorup Technology

  17. Demonstration of remotely operated TRU waste size reduction and material handling equipment

    International Nuclear Information System (INIS)

    Looper, M.G.; Charlesworth, D.L.

    1988-01-01

    The Savannah River Laboratory (SRL) is developing remote size reduction and material handling equipment to prepare 238 Pu contaminated waste for permanent disposal at the Waste Isolation Pilot Plant (WIPP) in New Mexico. The waste is generated at the Savannah River Plant (SRP) from normal operation and decommissioning activity and is retrievably stored onsite. A Transuranic Waste Facility for preparing, size-reducing, and packaging this waste for disposal is scheduled for completion in 1995. A cold test facility for demonstrating the size reduction and material handling equipment was built, and testing began in January 1987. 9 figs., 1 tab

  18. Demonstration of remotely operated tru waste size reduction and material handling equipment

    International Nuclear Information System (INIS)

    Looper, M.G.; Charlesworth, D.L.

    1988-01-01

    The Savannah River Laboratory (SRL) is developing remote size reduction and material handling equipment to prepare 238Pu and 239Pu contaminated waste for permanent disposal at the Waste Isolation Pilot Plant (WIPP) in New Mexico. The waste is generated at the Savannah River Plant (SRP) from normal operation and decommissioning activity and is retrievably stored on-site. A Transuranic Waste Facility for preparing, size-reducing, and packaging this waste for disposal is scheduled for completion in 1995. A cold test facility for demonstrating the size reduction and material handling equipment was built, and testing began in January 1987

  19. Materials Handling and Structures 01.0302 for Agribusiness, Natural Resources, and Environmental Occupations.

    Science.gov (United States)

    Finstad, Dennis; And Others

    The document presents unit plans which offer lists of experiences and competencies to be learned in the area of materials handling and structuring for agribusiness, natural resources, and environmental occupations. The units include: (1) farmstead planning and reorganization; (2) site preparation (contour, terraces, waterways; land measurements…

  20. Solar Energy: Materials, Materials Handling, and Fabrication Processes: Student Material. First Edition.

    Science.gov (United States)

    Bolin, William Everet; Orsak, Charles G., Jr.

    Designed for student use in "Materials, Materials Handling, and Fabrication Processes," one of 11 courses in a 2-year associate degree program in solar technology, this manual provides readings, exercises, worksheets, bibliographies, and illustrations for 13 course modules. The manual, which corresponds to an instructor guide for the…

  1. ENGINEERING BULLETIN: CONTROL OF AIR EMISSIONS FROM MATERIALS HANDLING DURING REMEDIATION

    Science.gov (United States)

    This bulletin presents an overview discussion on the importance of and methods for controlling emissions into the air from materials handling processes at Superfund or other hazardous waste sites. It also describes several techniques used for dust and vapor suppress ion that have...

  2. A conceptual framework for outsourcing of materials handling activities in automotive : differentiation and implementation

    NARCIS (Netherlands)

    Klingenberg, W.; Boksma, J. D.

    2010-01-01

    This article discusses the outsourcing of materials handling activities and investigates different options for its implementation. The article uses descriptive case studies found in literature from the Western European automotive industry to map out differences in current practice and to evaluate

  3. The cost analysis of material handling in Chinese traditional praying paper production plant

    Science.gov (United States)

    Nasution, H.; Budiman, I.; Salim, A.

    2018-02-01

    Chinese traditional praying paper industry is an industry which produced Chinese traditional religion praying paper. This kind of industry is rarely examined since it was only in Small and Medium Enterprise (SME’s- form). This industry produced various kinds of Chinese traditional paper products. The purpose of this research is to increase the amount of production, reduce waiting time and moving time, and reduce material handling cost. The research was conducted at prime production activities, consists of: calculate the capacity of the material handler, the frequency of movement, cost of material handling, and total cost of material handling. This displacement condition leads to an ineffective and inefficient production process. The alternative was developed using production judgment and aisle standard. Based on the observation results, it is possible to reduce displacement in the production. Using alternative which by-passed displacement from a rolled paper in the temporary warehouse to cutting and printing workstation, it can reduce material handling cost from 2.26 million rupiahs to 2.00 million rupiahs only for each batch of production. This result leads to increasing of production quantity, reducing waiting and moving time about 10% from the current condition.

  4. 29 CFR 1919.50 - Eligibility for accreditation to certificate shore-based material handling devices covered by...

    Science.gov (United States)

    2010-07-01

    ... material handling devices covered by § 1917.50 of this chapter, safety and health regulations for marine... Shore-Based Equipment § 1919.50 Eligibility for accreditation to certificate shore-based material handling devices covered by § 1917.50 of this chapter, safety and health regulations for marine terminals...

  5. Toxic and reactive material handling on Spacelab J and USML-1

    Science.gov (United States)

    Dashner, Jack

    1990-01-01

    Spacelab J and USML-1 provide prime examples of materials which are toxic at ambient conditions or toxic during the processing stages. The experimentation requirements are outlined in relation to toxicity and reactive materials handling. Triple containment is the preferred method for prevention of toxic material release in habitable areas for catastrophic hazards. The containments must be adequate for the intended use and environment. When operations preclude triple containment, innovative methods should be explored.

  6. [Manual material handling risk assessment and ergonomic improvement in foodstuff retailing company].

    Science.gov (United States)

    Maso, S; Maccà, I; Simonetti, A; Scopa, P; Paruzzolo, P; Bonacci, A; Murgolo, I; Bartolucci, G B

    2011-01-01

    The aim of this study is to assess and reduce the risk due to manual material handling in a company involved in the foodstuff retailing. The risk assessment was performed by NIOSH Variable Lifing Index in 13 different occupational conditions. As result the risk was present in any case, with VLI values ranging from 2.12 to 2.81. A good risk reduction has been accomplished correcting properly the most important multiplier involved in the computation of the revised NIOSH Lifting equation (lifting frequency and weight of heavier products). Even if the performed risk reduction has been significant, the residual risk is still higher than the level of acceptability.

  7. Age related differences in mechanical demands imposed on the lower back by manual material handling tasks.

    Science.gov (United States)

    Shojaei, Iman; Vazirian, Milad; Croft, Emily; Nussbaum, Maury A; Bazrgari, Babak

    2016-04-11

    The prevalence of low back pain (LBP) increases with age, yet the underlying mechanism(s) responsible for this remains unclear. To explore the role of biomechanical factors, we investigated age-related differences in lower-back biomechanics during sagittally-symmetric simulated manual material handling tasks. For each task, trunk kinematics and mechanical demand on the lower back were examined, from among 60 participants within five equal-sized and gender-balanced age groups spanning from 20 to 70 years old. The tasks involved lowering a 4.5 kg load from an upright standing posture to both knee height and a fixed height and then lifting the load back to the initial upright posture. During these tasks, segmental body kinematics and ground reaction forces were collected using wireless inertial measurement units and a force platform. Overall, older participants completed the tasks with larger pelvic rotation and smaller lumbar flexion. Such adopted trunk kinematics resulted in larger peak shearing demand at the lower back in older vs. younger participants. These results suggest that older individuals may be at a higher risk for developing lower back pain when completing similar manual material handling tasks, consistent with epidemiological evidence for higher risks of occupational low back pain among this cohort. Copyright © 2015 Elsevier Ltd. All rights reserved.

  8. A neural network model for predicting postures during non-repetitive manual materials handling tasks.

    Science.gov (United States)

    Perez, Miguel A; Nussbaum, Maury A

    2008-10-01

    Posture prediction can be useful in facilitating the design and evaluation processes for manual materials handling tasks. This study evaluates the ability of artificial neural network models to predict initial and final lifting postures in 2-D and 3-D scenarios. Descriptors for the participant and condition of interest were input to the models; outputs consisted of posture-defining joint angles. Models were trained with subsets of an existing posture database before predictions were generated. Trained models predictions were then evaluated using the remaining data, which included conditions not presented during training. Prediction errors were consistent across these data subsets, suggesting the models generalised well to novel conditions. The models generally predicted whole-body postures with per-joint errors in the 5 degrees -20 degrees range, though some errors were larger, particularly for 3-D conditions. These models provided reasonably accurate predictions, even outperforming some computational approaches previously proposed for similar purposes. Suggestions for future refinement of such models are presented. The models in this investigation provide a means to predict initial and final postures in commonly occurring manual materials handling tasks. In addition, the model structures provide information about potential lifting strategies that may be used by individuals with particular anthropometry or strength characteristics.

  9. Teknik Squat dan Stoop Menggunakan Electromyography pada Pekerjaan Manual Materials Handling

    Directory of Open Access Journals (Sweden)

    I Wayan Surata

    2013-01-01

    Full Text Available Manual materials handling has been identified as the most common cause of work-related musculoskeletal disorders. Among the manual material handling activities, lifting has long been regarded as an activity associated with a high risk of low-back pain. Lifting studies have mainly focused on the squat and stoop lifting techniques as an effort to improve the protection of workers from low back discomfort. However, neither is ideal and the benefits of one technique over another have proved inconclusive. The purpose of this study was to examine and compare the squat and stoop lifting techniques through analysis of muscles activation by using electromyography. Six volunteers participated in the study, and were required to lift a weight with squat and stoop techniques, with two types of loading at 1,7 kg and 6,7 kg. Observations were made on the rectus femoris, biceps femoris, and multifidus muscles. The results of study showed that the squat technique had higher levels of muscle activation compared to stoop technique on rectus femoris muscle. On the contrary, squat technique had lower muscle activation compared to stoop technique on biceps fermoris muscle. Meanwhile, both techniques squat and stoop had the same level of muscle activation on multifidus muscle. Conclusion, squat and stoop lifting techniques had the same opportunities to use.

  10. Comparison of new multi-criteria decision making methods for material handling equipment selection

    Directory of Open Access Journals (Sweden)

    Manoj Mathew

    2018-03-01

    Full Text Available Conveyors and automated guided vehicles (AGVs are important mechanical handling equipment used to transport loads from one place to another. Selection of proper material handling equipment is an intricate process. In the present paper two material handling equipment selection problem was solved using various newly developed multi criteria decision making (MCDM methods. First a conveyor selection problem with six conflicting criteria and four alternatives was solved using four MCDM methods, i.e. combinative distance based assessment (CODAS method, evaluation based on distance from average solution (EDAS method, weighted aggregated sum product assessment (WASPAS method and multi-objective optimization on the basis of ratio analysis (MOORA method. Second an automated guided vehicles selection problem with six conflicting criteria and eight alternatives was solved using CODAS, EDAS, WASPAS and MOORA methods. Spearman rank correlation coefficient was calculated between the ranks obtained by various methods. The ranks obtained by these methods were even compared with the ranks of other MCDM methods and it was found that the relatively new methods CODAS, EDAS and WASPAS were in good agree-ment with each other.

  11. Minimizing the carbon footprint of material handling equipment: Comparison of electric and LPG forklifts

    Directory of Open Access Journals (Sweden)

    Francesco Facchini

    2016-12-01

    Full Text Available Purpose: The aim of this study is to identify the best Material Handling Equipment (MHE to minimize the carbon footprint of inbound logistic activities, based on the type of the warehouse (layout, facilities and order-picking strategy as well as the weight of the loads to be handled. Design/methodology/approach: A model to select the best environmental MHE for inbound logistic activities has been developed. Environmental performance of the MHE has been evaluated in terms of carbon Footprint (CF. The model is tested with a tool adopting a VBA macro as well as a simulation software allowing the evaluation of energy and time required by the forklift in each phase of the material handling cycle: picking, sorting and storing of the items. Findings: Nowadays, it is not possible to identify ‘a priori’ a particular engine equipped forklift performing better than others under an environmental perspective. Consistently, the application of the developed model allows to identify the best MHE tailored to each case analyzed.   Originality/value: This work gives a contribution to the disagreement between environmental performances of forklifts equipped with different engines. The developed model can be considered a valid support for decision makers to identify the best MHE minimizing the carbon footprint of inbound logistic activities.

  12. Risk assessment of manual material handling activities (case study: PT BRS Standard Industry)

    Science.gov (United States)

    Deviani; Triyanti, V.

    2017-12-01

    The process of moving material manually has the potential for injury to workers. The risk of injury will increase if we do not pay attention to the working conditions. The purpose of this study is to assess and analyze the injury risk level in manual handling material activity, as well as to improve the condition. The observed manual material handling activities is pole lifting and goods loading. These activities were analyzed using Job Strain Index method, Rapid Entire Body Assessment, and Chaffin’s 2D Planar Static Model. The results show that most workers who perform almost all activities have a high level of risk level with the score of JSI and REBA exceeds 9 points. For some activities, the estimated compression forces in the lumbar area also exceed the standard limits of 3400 N. Concerning this condition, several suggestions for improvement were made, improving the composition of packing, improving body posture, and making guideline posters.

  13. Training in manual material handling: what is going on in the field?

    Science.gov (United States)

    Hermans, Veerle; De Preter, Griet; Verschueren, Tine

    2012-01-01

    During the last five years, several research review studies have revealed insufficient or even no proof that courses in manual material handling (MMH) are effective at preventing musculoskeletal disorders (MSD). These revelations are placing the current daily practice of MMH trainers under scrutiny. This study analyses how the MMH courses are organised in practice. A web-based questionnaire was developed for trainers. Only trainers giving MMH courses on a regular basis were included. The questionnaire focussed on general characteristics of the courses, content issues and organisational aspects. The results clearly indicated that the MMH courses can only be considered as a means of making employees aware of good manual handling. Not all content issues that should be addressed in effective MMH courses, are looked at in practice. This is mainly due to time restrictions that trainers experience, often imposed by the company. In conclusion, these organisational restrictions might explain the ineffectiveness of the MMH courses and should therefore be resolved.

  14. Oxygen consumption prediction models for individual and combination materials handling tasks.

    Science.gov (United States)

    Dempsey, Patrick G; Ciriello, Vincent M; Maikala, Rammohan V; O'Brien, Niall V

    2008-11-01

    An experiment was conducted to develop models to predict oxygen consumption of males and females engaged in common materials handling tasks including lifting, lowering, pushing, pulling, (de)palletising and combination tasks involving lifting or lowering a box and carrying it a set distance and lifting or lowering it to the destination. Nineteen male and 19 female subjects participated in the study. A psychophysical approach was used to set load limits for individual subjects for the oxygen consumption protocol. The 8398 oxygen consumption values collected were entered into the initial regression analyses and 168 potential outliers were removed before the final models were run. In addition to relevant task variables, body weight was a significant predictor variable in all models. The r(2) values for the final models ranged from 0.54 to 0.82 and the root mean square errors ranged from 90.2 ml to 294.8 ml.

  15. A case study on determining air monitoring requirements in a radioactive materials handling area

    International Nuclear Information System (INIS)

    Newton, G.J.; Bechtold, W.E.; Hoover, M.D.; Ghanbari, F.; Herring, P.S.; Jow, Hong-Nian

    1993-01-01

    A technical, defensible basis for the number and placement of air sampling instruments in a radioactive materials handling facility was developed. Historical air sampling data, process and physicochemical knowledge, qualitative smoke dispersion studies with video documentation, and quantitative trace gas dispersion studies were used to develop a strategy for number and placement of air samplers. These approaches can be used in other facilities to provide a basis for operational decisions. The requirements for retrospective sampling, personal sampling, and real-time monitoring are included. Other relevant operational decisions include selecting the numbers, placement, and appropriate sampling rates for instruments, identifying areas of stagnation or recirculation, and determining the adequacy and efficiency of any sampling transport lines. Justification is presented for using a graded approach to characterizing the workplace and determining air sampling and monitoring needs

  16. An evaluation of classification algorithms for manual material handling tasks based on data obtained using wearable technologies.

    Science.gov (United States)

    Kim, Sunwook; Nussbaum, Maury A

    2014-01-01

    With recent progress in wearable measurement systems, physical exposures can be feasibly assessed at high precision in the workplace. Such systems, however, generally lack contextual information for a given job (e.g., task type, duration). To extract such information, we explored three classification algorithms to classify manual material handling (MMH) tasks during a simulated job in a laboratory, using several combinations of outputs from commercially available inertial motion capture and in-shoe pressure measurement systems. A total of 10 participants completed three replications of four cycles of a simulated job. Precision and recall values of ≥ ∼90% and 80%, respectively, and errors in estimated task duration of < ∼14%, could be achieved across the MMH task examined. Classification performance, however, varied between classification algorithms, input data sets and task types. Overall, combining wearable technology with task classification could be an effective approach for field-based exposure assessment, though field-testing is needed to demonstrate the applicability of this method. Combining wearable technologies with task classification was explored to extract exposure context, specifically task type and duration. Results supported that task classification can facilitate the use of wearable technologies in field-based exposure assessment, specifically by aiding in task identification from within the rather large data sets obtained from these technologies.

  17. Estimating dynamic external hand forces during manual materials handling based on ground reaction forces and body segment accelerations.

    Science.gov (United States)

    Faber, Gert S; Chang, Chien-Chi; Kingma, Idsart; Dennerlein, Jack T

    2013-10-18

    Direct measurement of hand forces during assessment of manual materials handling is infeasible in most field studies and some laboratory studies (e.g., during patient handling). Therefore, this study proposed and evaluated the performance of a novel hand force estimation method based on ground reaction forces (GRFs) and body segment accelerations. Ten male subjects performed a manual lifting/carrying task while an optoelectronic motion tracking system measured 3D full body kinematics, a force plate measured 3D GRFs and an instrumented box measured 3D hand forces. The estimated 3D hand forces were calculated by taking the measured GRF vector and subtracting the force vectors due to weight and acceleration of all body segments. Root-mean-square difference (RMSD) between estimated and measured hand forces ranged from 11 to 27N. When ignoring the segment accelerations (just subtracting body weight from the GRFs), the hand force estimation errors were much higher, with RMSDs ranging from 21 to 101N. Future studies should verify the performance of the proposed hand force estimation method when using an ambulatory field measurement system. © 2013 Elsevier Ltd. All rights reserved.

  18. Biomechanical differences between expert and novice workers in a manual material handling task.

    Science.gov (United States)

    Plamondon, Andre; Denis, Denys; Delisle, Alain; Lariviere, Christian; Salazar, Erik

    2010-10-01

    The objective was to verify whether the methods were safer and more efficient when used by expert handlers than by novice handlers. Altogether, 15 expert and 15 novice handlers were recruited. Their task was to transfer four boxes from a conveyor to a hand trolley. Different characteristics of the load and lifting heights were modified to achieve a larger variety of methods by the participants. The results show that the net moments at the L5/S1 joint were not significantly different (p > 0.05) for the two groups. However, compared with the novices, the experts bent their lumbar region less (experts 54° (SD 11°); novices 66° (SD 15°)) but bent their knees more (experts approx. 72° (SD approx. 30°); novices approx. 53° (SD approx. 33°), which brought them closer to the box. The handler's posture therefore seems to be a major aspect that should be paid specific attention, mainly when there is maximum back loading. STATEMENT OF RELEVANCE: The findings of this research will be useful for improving manual material handling training programmes. Most biomechanical research is based on novice workers and adding information about the approach used by expert handlers in performing their tasks will help provide new avenues for reducing the risk of injury caused by this demanding physical task.

  19. Step scaling and behaviour selection in a constrained set of manual material handling transfers.

    Science.gov (United States)

    Wagner, David W; Reed, Matthew P

    2013-01-01

    Predictive biomechanical analysis of manual material handling (MMH) transfers is dependent on accurate prediction of foot locations relative to the task. Previous studies have classified common acyclic stepping patterns used during those transfer tasks, but the influence of walking distance prior to the transfer is not well understood. Twenty men and women performed transfers for a minimum of six different delivery distance conditions. The number of steps used by the participants ranged from two to seven. A theoretical framework for idealised step-scaling strategies is proposed and compared with the experimental data. The maximum observed increase in step length prior to delivery was 1.43 times the nominal step length calculated for each participant. The data suggest that although participants can scale their steps to facilitate the use of a single terminal stance at the transfer, the majority of participants chose to utilise a combination of stepping strategies if the preferred contralateral lead foot strategy could not be easily implemented. Accurate foot placements are needed for predictive biomechanical analysis of MMH. A laboratory study investigated the influence of previous step positions on MMH. A flexible step-scaling strategy, in which step lengths and strategy were varied, suggests that analysis based on simulated movements should consider multiple lifting postures.

  20. Evaluation and redesign of manual material handling in a vaccine production centre's warehouse.

    Science.gov (United States)

    Torres, Yaniel; Viña, Silvio

    2012-01-01

    This study was conducted in a warehouse at a vaccine production centre where improvement to existing storage and working conditions were sought through the construction of a new refrigerated store section (2-8C°). Warehousing tasks were videotaped and ergonomics analysis tools were used to assess the risk of developing MSDs. Specifically, these tools were the Rapid Entire Body Assessment (REBA) and the NIOSH equation. The current plant layout was sketched and analyzed to find possible targets for improvement trough the application of general work space design and ergonomics principles. Seven of the eight postures evaluated with REBA had a total score between 8 and 10, meaning a high risk, and only one was at a medium risk level. Nine of the eleven manual material handling tasks analyzed with the NIOSH equation had a Lifting Index between 1.14 and 1.80 and two had a recommended weight limit of 0 kg, indicating a need for job redesign. Solutions included the redesign of shelves, the design of a two-step stair and a trolley with adjustable height; also, changes in work methods were proposed by introducing a two-workers lifting strategy and job rotation, and, finally, a restructuring of plant layout was completed.

  1. Ergonomics intervention in a tile industry- case of manual material handling.

    Science.gov (United States)

    Dormohammadi, Ali; Amjad Sardrudi, Hosein; Motamedzade, Majid; Dormohammadi, Reza; Musavi, Saeed

    2012-12-13

    Manual material handling is one of the major health and safety hazards in industry. This study aims to assess the lifting tasks, before and after intervention using NIOSH lifting equation and Manual Handling Assessment Charts (MAC). This interventional study was performed in 2011 in a tile manufacturing industry in Hamadan, located in the West of Iran. The prevalence of musculoskeletal discomfort was determined using Nordic musculoskeletal questionnaire. In order to assess the risk factors related to lifting and identify the high-risk activities, MAC and NIOSH lifting equation were used. In intervention phase, we designed a load-carrying cart with shelves capable of moving vertically up and down, similar to scissor lifts. After intervention, the reassessment of risk factors was conducted to determine the success of the intervention and to compare risk levels before and after intervention using t-test. The outputs of MAC and NIOSH lifting equation assessments before intervention revealed that all activities were at high-risk level. After intervention, the risk level decreased to average level. In conclusion, the results of intervention revealed a considerable decrease in risk level. It may be concluded that the given intervention was acceptable and favorably effective in preventing musculoskeletal disorders especially low back pain.

  2. Effects of sitting and standing on upper extremity physical exposures in materials handling tasks.

    Science.gov (United States)

    Cudlip, Alan C; Callaghan, Jack P; Dickerson, Clark R

    2015-01-01

    Sitting or standing work configurations modulate musculoskeletal risk. Most existing investigations of these configurations have either studied them separately or lacked focus on the upper extremity, particularly during manual materials handling (MMH) tasks. To address this gap, upper extremity loading in 20 male and 20 females were assessed in 4 MMH tasks in sitting and standing. Differences in electromyographic (EMG) activity, local joint moments and body discomfort between configurations were examined. Interactions between task and sit/stand configuration resulted in increases of up to 500% in joint moments, 94% in EMG activity and 880% in discomfort when tasks were completed while sitting (p < 0.01). Future MMH task designers should consider placing workers in standing postures when feasible to reduce upper extremity loading, but workers should not remain in either configuration for extended periods of time as the negative effects of both workspace geometries can instigate future musculoskeletal disorders. Practitioner Summary: Sitting and standing modify occupational musculoskeletal risk. We examined how performing identical tasks while sitting or standing altered upper extremity and low back loading. In general, sitting increased muscle activity and discomfort, while standing increased local joint moments. The benefits of standing outweighed those of sitting across the range of tasks.

  3. Manual materials handling: knowledge and practices among Portuguese health and safety practitioners.

    Science.gov (United States)

    Arezes, Pedro M; Miguel, A Sérgio; Colim, Ana S

    2011-01-01

    There are several methodologies to assess the risk involved in Manual Materials Handling (MMH). The aim of this study was to identify the knowledge and use of risk assessment methods among Portuguese Health and Safety (H&S) practitioners. The study involved the participation of 331 H&S practitioners and other related prevention professionals. An e-mail invitation to complete an online survey was sent to all Portuguese registered H&S practitioners. The previously developed and validated questionnaire included three different sections: (1) company characteristics; (2) description of types of MMH tasks performed; and (3) the respondents' use of and knowledge about risk assessment methods for MMH tasks. The answers obtained were collected from almost 350 companies. The characteristics of the companies showed that there is a predominance of external and internal H&S services for Small and Medium sized Enterprises (SMEs) and large companies, respectively. The NIOSH equation, Key Indicator Method (KIM) and Manual Assessment Chart (MAC) were identified as the most used and recognised risk assessment methods. Questionnaire results show that an important number of Portuguese H&S practitioners recognise some methods but have difficulty applying them correctly. Some practitioners reported that they had never used any type of risk assessment method.

  4. A relation between dynamic strength and manual materials-handling strategy affected by knowledge of strength.

    Science.gov (United States)

    Bartlett, Daniel; Li, Kang; Zhang, Xudong

    2007-06-01

    We studied the relation between dynamic (isokinetic) strength and the batch-assorting strategy to initiate a manual materials-handling task and the effect of knowledge of strength on that relation. The debated, complex relationship between muscular strength and the risk of injury can be better understood from a behavioral perspective by examining performance strategies in physical acts such as lifting. Thirty-two participants (16 men and 16 women) were first tested for their isokinetic strengths of trunk extension, knee extension, shoulder extension, and shoulder abduction. The participants were then divided into two groups, one provided with knowledge feedback of their strength testing results and the other not provided with such feedback. Participants subsequently performed the same load-handling task in which they carried batches of various weight plates while allowed to assort batches of more than one plate into any combination. Dynamic strength, as represented by a total isokinetic strength score, and knowledge feedback both had significant effects on measures quantifying the batch-assorting strategy. Individuals with greater strength tended to adopt a strategy corresponding to a heavier load per carry and fewer carries per batch. Receiving knowledge feedback evoked a tendency toward handling a heavier load, and this tendency was more salient in the weaker individuals. Potential applications include the use of strength testing in worker selection and training as well as in job design to promote better strategies of balancing productivity and injury prevention.

  5. Improving the work position of worker based on manual material handling in rice mill industry

    Science.gov (United States)

    Astuti, Rahmaniyah Dwi; Susmartini, Susy; Kinanthi, Ade Putri

    2017-11-01

    In traditional industries still using manual material handling to weight lifting. Worker at the rice mill, especially in rice filtering activity has wrong ergonomic posture to enforce the body bends and carried loads too heavy cause of injury for lower back and waist. The work attitude is unnatural posture. This study aimed to determine the severity of the workload, the level of risk posed to the rice taking activities and suggested as an improvement to it. Identify the operator complaints used Nordic Body Map method. Rapid Entire Body Assessment (REBA) method is used to provide an assessment of the working posture of the operator. Assessment of the working posture on rice filtering process shows that REBA score is 12 with an explanation very high level of risk and action level is 4 which means the action needs to be repaired immediately. Biomechanics calculation shows result 6713.21 N, the result of the calculation of the biomechanics of worker in the rice filtering activities indicates that the activities would pose a risk or injury. Therefore, improvement in rice filtering activity by designing a tool for lowering the risk level worker. The design tools are illustrated with 2D modeling resulted in the level of risk that is working REBA score became 3 which shows a low risk level. Biomechanics calculation after designed of tools show the result is 6282.86 N. The results means the activities carried out are still in safe condition and does not pose a risk or injury.

  6. Continuous ambulatory hand force monitoring during manual materials handling using instrumented force shoes and an inertial motion capture suit

    NARCIS (Netherlands)

    Faber, G. S.; Koopman, A. S.; Kingma, I.; Chang, C. C.; Dennerlein, J. T.; van Dieën, J. H.

    2017-01-01

    Hand forces (HFs) are commonly measured during biomechanical assessment of manual materials handling; however, it is often a challenge to directly measure HFs in field studies. Therefore, in a previous study we proposed a HF estimation method based on ground reaction forces (GRFs) and body segment

  7. Musculoskeletal symptoms associated with posterior load carriage: An assessment of manual material handling workers in Indonesia.

    Science.gov (United States)

    Muslim, Khoirul; Nussbaum, Maury A

    2015-06-05

    Concerns have been raised regarding the high prevalence of musculoskeletal symptoms (MSS) among manual material handling (MMH) workers. However, limited investigations have been undertaken among one large group of workers using a particular MMH method called posterior load carriage (PLC). This is typically done without the use of a backpack in developing countries, and involves exposure to known risk factors for MSS such as heavy loads, non-neutral postures, and high levels of repetition. To 1) determine the types and prevalence of MSS among PLC workers and the impacts of these MSS on workers, 2) explore job demands potentially contributing to MSS, and (3) obtain input from workers regarding possible improvements to facilitate future interventions. Structured interview applied to 108 workers to assess PLC worker characteristics and job demands in eight cities in Indonesia. MSS were reported in all anatomical regions evaluated, with symptoms most commonly reported at the lower back (72.2%), feet (69.4%), knees (64%), shoulders (47.2%), and neck (41.7%). Logistic regression indicated that MSS in the lower back were associated with longer work hours/day, MSS in the hands were associated with load mass, and MSS in the ankles/feet were associated with stature and load carriage frequency. MSS were reported to interfere with daily activity, but only few workers sought medical treatment. Possible improvements included the use of a belt, hook, or backpack/frame, and changes in the carriage method. The study suggests that PLC workers incur a relatively high MSS burden. Future studies are needed to develop and evaluate practical interventions and specific guidelines to improve working conditions and occupational health and safety for PLC workers.

  8. Comparison of Customer Preference for Bulk Material Handling Equipment through Fuzzy-AHP Approach

    Science.gov (United States)

    Sen, Kingshuk; Ghosh, Surojit; Sarkar, Bijan

    2017-06-01

    In the present study, customer's perception has played one of the important roles for selection of the exact equipment out of available alternatives. The present study is dealt with the method of optimization of selection criteria of a material handling equipment, based on the technical specifications considered to be available at the user end. In this work, the needs of customers have been identified and prioritized, that lead to the selection of number of criteria, which have direct effect upon the performance of the equipment. To check the consistency of selection criteria, first of all an AHP based methodology is adopted with the identified criteria and available product categories, based upon which, the judgments of the users are defined to derive the priority scales. Such judgments expressed the relative strength or intensity of the impact of the elements of the hierarchy. Subsequently, all the alternatives have ranked for each identified criteria with subsequent constitution of weighted matrices. The same has been compared with the normalized values of approximate selling prices of the equipments to determine individual cost-benefit ratio. Based on the cost-benefit ratio, the equipment is ranked. With same conditions, the study is obtained again with a Fuzzy AHP concept, where a fuzzy linguistic approach has reduced the amount of uncertainty in decision making, caused by conventional AHP due to lack of deterministic approach. The priority vectors of category and criteria are determined separately and multiplied to obtain composite score. Subsequently, the average of fuzzy weights was determined and the preferences of equipment are ranked.

  9. Analysis of manual material handling activity to increase work productivity (Case study: manufacturing company

    Directory of Open Access Journals (Sweden)

    Suryoputro Muhammad Ragil

    2018-01-01

    Full Text Available Manual material handling is one of work activities that have an effect on the physical aspect of workers in manufacturing industry, it is necessary to do the analysis of the risks from such activities. Analysis was performed on worker when performing manual lifting activity and when using two tools (Automatic Handlift and Manual Handlift. In addition to analyse in ergonomics aspect, time study analysis and productivity measurement were carried out to determine the effects of the equipment. Nordic Body Map (NBM questionnaire on worker using Automatic Handlift showed declining level of musculoskeletal disorders by 22%. REBA method obtained score of 10 and was declined to 4 after using these tool. The results of MPL method showed declining of Fc value from 4756.37 N to 1346.56 N. The results of RWL method showed declining of LI value (Lifting Index origin and destination from 1.84 and 1.18 to 1.12 and 0.89 respectively. As for worker using Manual Handlift, NBM questionnaire result shows declining level of musculoskeletal disorders by 57%. REBA method obtained scores of 8 and was reduced to 5. For MPL methode result, the Fc value is 4906.99 N and reduced to 2047.88 N. RWL method results showed declining of LI value (Lifting Index origin and destination from 1.02 and 0.67 to 0.74 and 0.58. The results of time study analysis showed declining of Standard Time when use the two tools and make the increasing productivity of 9% by worker using Automatic Hadlift and 4% by worker using Manual Handlift.

  10. Continuous ambulatory hand force monitoring during manual materials handling using instrumented force shoes and an inertial motion capture suit.

    Science.gov (United States)

    Faber, G S; Koopman, A S; Kingma, I; Chang, C C; Dennerlein, J T; van Dieën, J H

    2018-03-21

    Hand forces (HFs) are commonly measured during biomechanical assessment of manual materials handling; however, it is often a challenge to directly measure HFs in field studies. Therefore, in a previous study we proposed a HF estimation method based on ground reaction forces (GRFs) and body segment accelerations and tested it with laboratory equipment: GFRs were measured with force plates (FPs) and segment accelerations were measured using optical motion capture (OMC). In the current study, we evaluated the HF estimation method based on an ambulatory measurement system, consisting of inertial motion capture (IMC) and instrumented force shoes (FSs). Sixteen participants lifted and carried a 10-kg crate from ground level while 3D full-body kinematics were measured using OMC and IMC, and 3D GRFs were measured using FPs and FSs. We estimated 3D hand force vectors based on: (1) FP+OMC, (2) FP+IMC and (3) FS+IMC. We calculated the root-mean-square differences (RMSDs) between the estimated HFs to reference HFs calculated based on crate kinematics and the GRFs of a FP that the crate was lifted from. Averaged over subjects and across 3D force directions, the HF RMSD ranged between 10-15N when using the laboratory equipment (FP + OMC), 11-18N when using the IMC instead of OMC data (FP+IMC), and 17-21N when using the FSs in combination with IMC (FS + IMC). This error is regarded acceptable for the assessment of spinal loading during manual lifting, as it would results in less than 5% error in peak moment estimates. Copyright © 2017 Elsevier Ltd. All rights reserved.

  11. Manual material handling advice and assistive devices for preventing and treating back pain in workers.

    Science.gov (United States)

    Verbeek, Jos H; Martimo, Kari-Pekka; Karppinen, Jaro; Kuijer, P Paul Fm; Viikari-Juntura, Eira; Takala, Esa-Pekka

    2011-06-15

    Training and the provision of assistive devices are considered major interventions to prevent back pain and its related disability among workers exposed to manual material handling (MMH). To determine the effectiveness of MMH advice and training and the provision of assistive devices in preventing and treating back pain. We searched CENTRAL (The Cochrane Library 2011, issue 1), MEDLINE, EMBASE, CINAHL, Nioshtic, CISdoc, Science Citation Index, and PsychLIT to February 2011. We included randomised controlled trials (RCT) and cohort studies with a concurrent control group that were aimed at changing human behaviour in MMH and measured back pain, back pain-related disability or sickness absence. Two authors independently extracted the data and assessed the risk of bias using the criteria recommended by the Cochrane Back Review Group for RCTs and MINORS for the cohort studies.We based the results and conclusions on the analysis of RCTs only. We compared these with the results from cohort studies. We included nine RCTs (20,101 employees) and nine cohort studies (1280 employees) on the prevention of back pain in this updated review. Studies compared training to no intervention (4), professional education (2), a video (3), use of a back belt (3) or exercise (2). Other studies compared training plus lifting aids to no intervention (3) and to training only (1). The intensity of training ranged from a single educational session to very extensive personal biofeedback.Six RCTs had a high risk of bias.None of the included studies showed evidence of a preventive effect of training on back pain.There was moderate quality evidence from seven RCTs (19,317 employees) that those who received training reported levels of back pain similar to those who received no intervention, with an odds ratio of 1.17 (95% confidence intervals (CI) 0.68 to 2.02) or minor advice (video), with a relative risk of 0.93 (95% CI 0.69 to 1.25). Confidence intervals around the effect estimates were still

  12. Pengujian dan Perbaikan Desain Material Handling Equipment Buncis di PT Abofarm untuk Meningkatkan Efisiensi Kerja Menggunakan Metode Pengembangan Produk Ulrich Eppinger

    OpenAIRE

    Pura, I Gede Wisuda; Anugraha, Rino Andias; Yekti, Yusuf Nugroho Doyo

    2016-01-01

    Perancangan material handling equipment yang ergonomi merupakan salah satu cara untuk mengatasi musculoskeletal disorders (MSDs). Hasil implementasi desain material handling equipment menunjukkan beberapa masalah antara lain tingkat keberasilan operator dalam menggunakan alat 57%, jumlah kesalahan penggunaan alat yang besar, terdapat keluhan operator terkait teknis alat, Hal tersebut menunjukkan perlu perbaikan pada desain produk. Pada penelitian ini perbaikan desain menggunakan metode pengem...

  13. Perancangan Meja Adjustable Dengan Memperhatikan Postur Kerja Pekerja Manual Material Handling (Studi Kasus Di PT. Coca – Cola Bottling Indonesia)

    OpenAIRE

    Saptadi, Singgih; Wijanarko, Dwi

    2008-01-01

    PT Coca Cola Bottling Indonesia adalah salah satu Perusahaan yang bergerak dalam bidang minuman ringan baik berupa carbonated soft drink (coca-cola, fanta, sprite) maupun non-carbonated soft drink (frestea, ades). Penanganan material pada pabrik ini masih dilakukan secara manual (Manual Material Handling/MMH). Pekerjaan ini dimulai dengan pengangkatan kontainer dari atas conveyor kemudian dipindahkan dan disusun di atas pallet (proses palletizing).Rapid Entire Body Assessment (REBA) adalah sa...

  14. PERANCANGAN MEJA ADJUSTABLE DENGAN MEMPERHATIKAN POSTUR KERJA PEKERJA MANUAL MATERIAL HANDLING (Studi Kasus di PT. Coca – Cola Bottling Indonesia)

    OpenAIRE

    Singgih Saptadi; Dwi Wijanarko

    2012-01-01

    PT Coca Cola Bottling Indonesia adalah salah satu perusahaan yang bergerak dalam bidang minuman ringan baik berupa carbonated soft drink (coca-cola, fanta, sprite) maupun non-carbonated soft drink (frestea, ades). Penanganan material pada pabrik ini masih dilakukan secara manual (Manual Material Handling/MMH). Pekerjaan ini dimulai dengan pengangkatan kontainer dari atas conveyor kemudian dipindahkan dan disusun di atas pallet (proses palletizing).Rapid Entire Body Assessment (REBA) adalah sa...

  15. Effect of a worktable position on head and shoulder posture and shoulder muscles in manual material handling.

    Science.gov (United States)

    Kim, Min-Hee; Yoo, Won-Gyu

    2015-06-05

    According to a recent research, manual working with high levels of static contraction, repetitive loads, or extreme working postures involving the neck and shoulder muscles causes an increased risk of neck and shoulder musculoskeletal disorders. We investigated the effects of the forwardly worktable position on head and shoulder angles and shoulder muscle activity in manual material handling tasks. The forward head and shoulder angles and the activity of upper trapezius, levator scapulae, and middle deltoid muscle activities of 15 workers were measured during performing of manual material handling in two tasks that required different forward head and shoulder angles. The second manual material task required a significantly increased forward head and shoulder angle. The upper trapezius and levator scapulae muscle activity in second manual material task was increased significantly compared with first manual material task. The middle deltoid muscle activity in second manual material task was not significantly different compared with first manual material task. Based on this result, the forward head and shoulder angles while performing manual work need to be considered in selection of the forward distance of a worktable form the body. The high level contractions of the neck and shoulder muscles correlated with neck and shoulder pain. Therefore, the forward distance of a worktable can be an important factor in preventing neck and shoulder pain in manual material handling workers.

  16. Foot motions in manual material handling transfer tasks: a taxonomy and data from an automotive assembly plant.

    Science.gov (United States)

    Wagner, David W; Kirschweng, Rebecca L; Reed, Matthew P

    2009-03-01

    Ergonomic job analysis commonly applies static postural and biomechanical analysis tools to particular postures observed during manual material handling (MMH) tasks, usually focusing on the most extreme postures or those involving the highest loads. When these analyses are conducted prospectively using digital human models, accurate prediction of the foot placements is critical to realistic postural analyses. In automotive assembly jobs, workers frequently take several steps between task elements, for example, picking up a part at one location and moving to another location to place it on the vehicle. A detailed understanding of the influence of task type and task sequence on the stepping pattern is necessary to accurately predict the foot placements associated with MMH tasks. The current study examined the patterns of foot motions observed during automotive assembly tasks. Video data for 529 pickup and delivery tasks from 32 automotive assembly jobs were analysed. A minimum of five cycles was analysed for each task. The approach angle, departure angle, hand(s) used, manipulation height and patterns of footsteps were coded from the video. Object mass was identified from the job information sheet provided by the assembly plant. Three independent raters coded each video and demonstrated an intraclass correlation coefficient of 0.54 for identification of the configuration of the lower extremities during terminal stance. Based on an analysis of the distribution of stepping behaviours during object transitions (pickups or deliveries), a transition classification system (TRACS) was developed. TRACS uses a compact notation to quantify the sequence of steps associated with a MMH transition. Five TRACS behaviour groups accounted for over 90% of the transition stepping behaviours observed in the assembly plant. Approximately two-thirds (68.4%) of the object transfers observed were performed with only one foot in contact with the ground during the terminal posture. The

  17. How do low/high height and weight variation affect upper limb movements during manual material handling of industrial boxes?

    Directory of Open Access Journals (Sweden)

    Ana B. Oliveira

    Full Text Available OBJECTIVES: To evaluate the effect of surface height and load weight on upper limb movements and electromyographic (EMG recordings during manual handling performed by both experienced and inexperienced lifter subjects. METHODS: Sixteen experienced and sixteen inexperienced lifters handled a box (both 7 and 15 kg from an intermediate height (waist level to either a high or low surface. Electromyography and video images were recorded during the tasks. The 10th, 50th and 90th percentiles were calculated for the deltoid and biceps muscles, shoulder flexion, shoulder abduction, and elbow flexion movements. Groups, right/left sides, weights and heights were compared. There were no differences between either groups or sides. RESULTS: Weight and height variations affected EMG and posture, although weight had more impact on EMG. Shoulder abduction and flexion movements higher than 60º occurred, particularly for the higher surface. Shoulder flexion was also higher when the box was moved to the low height. This study provides new evidence as shoulder postures during boxes handling on low surfaces had not previously been evaluated. CONCLUSIONS: The high demand of upper limb in manual material handling tasks is clear, particularly for the shoulder. This knowledge can be used by physical therapists to plan better rehabilitation programs for manual material handling-related disorders, particularly focusing on return to work.

  18. How do low/high height and weight variation affect upper limb movements during manual material handling of industrial boxes?

    Science.gov (United States)

    Oliveira, Ana B; Silva, Luciana C C B; Coury, Helenice J C G

    2011-01-01

    To evaluate the effect of surface height and load weight on upper limb movements and electromyographic (EMG) recordings during manual handling performed by both experienced and inexperienced lifter subjects. Sixteen experienced and sixteen inexperienced lifters handled a box (both 7 and 15 kg) from an intermediate height (waist level) to either a high or low surface. Electromyography and video images were recorded during the tasks. The 10th, 50th and 90th percentiles were calculated for the deltoid and biceps muscles, shoulder flexion, shoulder abduction, and elbow flexion movements. Groups, right/left sides, weights and heights were compared. There were no differences between either groups or sides. Weight and height variations affected EMG and posture, although weight had more impact on EMG. Shoulder abduction and flexion movements higher than 60º occurred, particularly for the higher surface. Shoulder flexion was also higher when the box was moved to the low height. This study provides new evidence as shoulder postures during boxes handling on low surfaces had not previously been evaluated. The high demand of upper limb in manual material handling tasks is clear, particularly for the shoulder. This knowledge can be used by physical therapists to plan better rehabilitation programs for manual material handling-related disorders, particularly focusing on return to work.

  19. PERANCANGAN MEJA ADJUSTABLE DENGAN MEMPERHATIKAN POSTUR KERJA PEKERJA MANUAL MATERIAL HANDLING (Studi Kasus di PT. Coca – Cola Bottling Indonesia

    Directory of Open Access Journals (Sweden)

    Singgih Saptadi

    2012-02-01

    Full Text Available PT Coca Cola Bottling Indonesia adalah salah satu perusahaan yang bergerak dalam bidang minuman ringan baik berupa carbonated soft drink (coca-cola, fanta, sprite maupun non-carbonated soft drink (frestea, ades. Penanganan material pada pabrik ini masih dilakukan secara manual (Manual Material Handling/MMH. Pekerjaan ini dimulai dengan pengangkatan kontainer dari atas conveyor kemudian dipindahkan dan disusun di atas pallet (proses palletizing.Rapid Entire Body Assessment (REBA adalah salah satu metode yang digunakan untuk menilai postur kerjakaitanya dengan cedera otot akibat bekerja. Pada metode ini juga dipengaruhi oleh faktor coupling, beban eksternal serta aktivitas pekerja yang dialami oleh subjek pengamatan. Implementasi REBA dilakukan pada postur penanganan material khususnya pada pengangkatan krat dan penyusunan krat. Dari hasil penilaian menunjukkan action level didominasi pada level 3 dan level 4, untuk itu perlu dilakukan tindakan perbaikan segera/saat itu juga. Dalam melakukan rekomendasi perbaikan postur penulis melakukan perancangan fasilitas yang disesuaikan dengan data antropometri dan didasarkan pada jenis aktifitasnya serta memberikan usulan fasilitas berupa meja adjustable. Penulis memvisualisasikan hasil rancangan melalui sofware 3DS max, dimana sofware ini juga digunakan sebagai analisis postur pada fasilitas kerja usulan. Kata kunci : Manual Material Handling, REBA, perancangan fasilitas kerja, meja adjustable, 3DS max                Coca Cola Bottling Indonesia is one of the company which move on soft drink areas like carbonated soft drinks (coca-cola, fanta, sprite or non-carbonated soft drinks (frestea,ades. Material handling in this factory is still done manually. This work is started by lifting the container from conveyor  then move it and arrange it above the pallet ( proses palletizing.              Rapid Entire Body Assesment ( REBA is one of the method by which is used to score working

  20. Machine assembly with a new material handling mechanism in the sewing machine

    Directory of Open Access Journals (Sweden)

    Umarova Z.M.

    2017-05-01

    Full Text Available the paper presents the dynamic model of the machine assembly with a recommended mechanism for moving material and the definition of the law of rails motion under various system parameters. The author has suggested the solution implemented by the system of differential equations numerically on the PC and the system describing the motion of the machine set. Recommended values ​​of the parameters of elastic links of material transfer mechanism have been obtained. The researcher has developed the methods of kinematic and dynamic analysis of the material transfer mechanism with elastic elements of the sewing machine and has approved the parameters and development of the design.

  1. The development of a model to predict the effects of worker and task factors on foot placements in manual material handling tasks.

    Science.gov (United States)

    Wagner, David W; Reed, Matthew P; Chaffin, Don B

    2010-11-01

    Accurate prediction of foot placements in relation to hand locations during manual materials handling tasks is critical for prospective biomechanical analysis. To address this need, the effects of lifting task conditions and anthropometric variables on foot placements were studied in a laboratory experiment. In total, 20 men and women performed two-handed object transfers that required them to walk to a shelf, lift an object from the shelf at waist height and carry the object to a variety of locations. Five different changes in the direction of progression following the object pickup were used, ranging from 45° to 180° relative to the approach direction. Object weights of 1.0 kg, 4.5 kg, 13.6 kg were used. Whole-body motions were recorded using a 3-D optical retro-reflective marker-based camera system. A new parametric system for describing foot placements, the Quantitative Transition Classification System, was developed to facilitate the parameterisation of foot placement data. Foot placements chosen by the subjects during the transfer tasks appeared to facilitate a change in the whole-body direction of progression, in addition to aiding in performing the lift. Further analysis revealed that five different stepping behaviours accounted for 71% of the stepping patterns observed. More specifically, the most frequently observed behaviour revealed that the orientation of the lead foot during the actual lifting task was primarily affected by the amount of turn angle required after the lift (R(2) = 0.53). One surprising result was that the object mass (scaled by participant body mass) was not found to significantly affect any of the individual step placement parameters. Regression models were developed to predict the most prevalent step placements and are included in this paper to facilitate more accurate human motion simulations and ergonomics analyses of manual material lifting tasks. STATEMENT OF RELEVANCE: This study proposes a method for parameterising the steps

  2. Proposed master-slave and automated remote handling system for high-temperature gas-cooled reactor fuel refabrication

    International Nuclear Information System (INIS)

    Grundmann, J.G.

    1974-01-01

    The Oak Ridge National Laboratory's Thorium-Uranium Recycle Facility (TURF) will be used to develop High-Temperature Gas-Cooled Reactor (HTGR) fuel recycle technology which can be applied to future HTGR commercial fuel recycling plants. To achieve recycle capabilities it is necessary to develop an effective material handling system to remotely transport equipment and materials and to perform maintenance tasks within a hot cell facility. The TURF facility includes hot cells which contain remote material handling equipment. To extend the capabilities of this equipment, the development of a master-slave manipulator and a 3D-TV system is necessary. Additional work entails the development of computer controls to provide: automatic execution of tasks, automatic traverse of material handling equipment, automatic 3D-TV camera sighting, and computer monitoring of in-cell equipment positions to prevent accidental collisions. A prototype system which will be used in the development of the above capabilities is presented. (U.S.)

  3. Real-time software use in nuclear materials handling criticality safety control

    International Nuclear Information System (INIS)

    Huang, S.; Lappa, D.; Chiao, T.; Parrish, C.; Carlson, R.; Lewis, J.; Shikany, D.; Woo, H.

    1997-01-01

    This paper addresses the use of real-time software to assist handlers of fissionable nuclear material. We focus specifically on the issue of workstation mass limits, and the need for handlers to be aware of, and check against, those mass limits during material transfers. Here ''mass limits'' generally refer to criticality safety mass limits; however, in some instances, workstation mass limits for some materials may be governed by considerations other than criticality, e.g., fire or release consequence limitation. As a case study, we provide a simplified reliability comparison of the use of a manual two handler system with a software-assisted two handler system. We identify the interface points between software and handlers that are relevant to criticality safety

  4. Translation in cell-free systems

    International Nuclear Information System (INIS)

    Jagus, R.

    1987-01-01

    The simplest, unambiguous identification of a particular mRNA is the identification of its protein product. This can be established by translation of the mRNA of interest in a cell-free protein-synthesizing system. Messenger RNA protein product identification is important in the isolation of a particular mRNA species for cDNA cloning and in the identification of positive cDNA clones. The two high-activity translation systems in common use are those prepared from rabbit reticulocytes and from wheat germ. Both systems are easy to prepare, and both are available commercially. Each has advantages and disadvantages over the other and a choice between the two will depend on the type of mRNAs to be translated, the prejudices of experience, and availability. The main disadvantage of the reticulocyte system is that it requires removal of endogenous mRNA. However, this is a relatively simple procedure. The wheat germ system does not require removal of endogenous mRNA and may translate weakly initiating mRNAs more efficiently. However, ionic optima for translation in the wheat germ system are more sensitive to the nature and concentration of mRNA and may need to be determined for each template. The biggest problem with the use of the wheat germ system is its tendency to produce incomplete translation products due to premature termination

  5. The Department of Energy`s Rocky Flats Plant: A guide to record series useful for health related research. Volume 4: Production and materials handling

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1995-08-01

    This is the fourth in a series of seven volumes which constitute a guide to records of the Rocky Flats Plant useful for conducting health-related research. The primary purpose of Volume 4 is to describe record series pertaining to production and materials handling activities at the Department of Energy`s (DOE) Rocky Flats Plant, now named the Rocky Flats Environmental Technology Site, near Denver, Colorado. History Associates Incorporated (HAI) prepared this guide as part of its work as the support services contractor for DOE`s Epidemiologic Records Inventory Project. This introduction briefly describes the Epidemiologic Records Inventory Project and HAI`s role in the project, provides a history of production and materials handling practices at Rocky Flats, and identifies organizations contributing to production and materials handling policies and activities. Other topics include the scope and arrangement of the guide and the organization to contact for access to these records.

  6. Effects of restrictive clothing on lumbar range of motion and trunk muscle activity in young adult worker manual material handling.

    Science.gov (United States)

    Eungpinichpong, Wichai; Buttagat, Vitsarut; Areeudomwong, Pattanasin; Pramodhyakul, Noppol; Swangnetr, Manida; Kaber, David; Puntumetakul, Rungthip

    2013-11-01

    The objective of this study was to examine the effect of wearing restrictive trousers on lumbar spine movement, trunk muscle activity and low back discomfort (LBD) in simulations of manual material handling (MMH) tasks. Twenty-eight young adults participated in the study performing box lifting, liquid container handling while squatting, and forward reaching while sitting on a task chair when wearing tight pants (sizes too small for the wearer) vs. fit pants (correct size according to anthropometry). Each task was repeated three times and video recordings were used as a basis for measuring lumbar range of motion (LRoM). The response was normalized in terms on baseline hip mobility. Trunk muscle activity of rectus abdominis (RA) and erector spinae (ES) muscles were also measured in each trial and normalized. At the close of each trial, participants rated LBD using a visual analog scale. Results revealed significant effects of both pants and task types on the normalized LRoM, trunk muscle activity and subjective ratings of LBD. The LRoM was higher and trunk muscle (ES) activity was lower for participants when wearing tight pants, as compared to fit pants. Discomfort ratings were significantly higher for tight pants than fit. These results provide guidance for recommendations on work clothing fit in specific types of MMH activities in order to reduce the potential of low-back pain among younger workers in industrial companies. Copyright © 2013 Elsevier Ltd and The Ergonomics Society. All rights reserved.

  7. Two-dimensional biomechanical model for estimating strength of youth and adolescents for manual material handling tasks.

    Science.gov (United States)

    Waters, Thomas R; Garg, Arun

    2010-01-01

    Youth and adolescents are routinely engaged in manual material handling (MMH) tasks that may exceed their strength capability to perform the task and may place them at excessive risk for musculoskeletal disorders. This paper reports on a two-dimensional biomechanical model that was developed to assess MMH tasks performed by youth 3-21 years of age. The model uses age, gender, posture of the youth performing the MMH activity, and weight of the load handled as input, and provides an estimate of the strength demands of the task and spinal disc compression and shear force resulting from the activity as output. The model can be used to assess whether a specific MMH task exceeds the strength demands for youth of certain ages or genders, which of the internal muscle strengths are most affected, and provides information about the estimated spinal disc compression and shear forces on the spine as a result of the specified MMH task. These results would be helpful in deciding whether a task is appropriate for a youth to perform or whether a certain task modification may be sufficient in reducing the physical demands to a level acceptable for a youth of certain age and gender.

  8. Gender involvement in manual material handling (mmh) tasks in agriculture and technology intervention to mitigate the resulting musculoskeletal disorders.

    Science.gov (United States)

    Singh, Suman; Sinwal, Neelima; Rathore, Hemu

    2012-01-01

    The lifting and carrying of loads in agriculture on small landholdings are unavoidable. Rural communities often lack access to appropriate technologies which may result in various health hazards. The objective was to study gender participation in agricultural activities involving manual material handling tasks, to assess MSDs experienced in various MMH tasks and to evaluate traditional method and designed technology. The study was conducted on 100 agricultural workers. Data on gender participation in MMH tasks in household, animal husbandry and agriculture and resulting MSDs was gathered. Pre and post assessment of technology intervention was done for NIOSH Lifting Index, QEC, and RPE. The results revealed greater susceptibility of females to musculoskeletal problems in most of the household and animal husbandry tasks. The hand trucks designed were pushing type with power grasp handle. The respondents were advised to carry 5 kg of weight per lift instead of lifting more weight in one lift/minute while filling the hand truck. By decreasing the weight and increasing the number of lifts per minute the respondents were seen falling in green zone indicating significant reduction in NIOSH lifting index. QEC scores concluded that for filling the hand truck 5 kg of weight should be carried to keep the exposure level low.

  9. Lower back pain and its association with whole-body vibration and manual materials handling among commercial drivers in Sabah.

    Science.gov (United States)

    Awang Lukman, Khamisah; Jeffree, Mohammad Saffree; Rampal, Krishna Gopal

    2018-02-16

    A cross-sectional study was undertaken to determine the prevalence of lower back pain (LBP) and its association with whole-body vibration (WBV) and manual materials handling (MMH). We studied 110 commercial vehicle drivers using a self-administered questionnaire and the VI-400Pro human vibration monitor. Prevalence of LBP was 66.4%. The percentage of drivers who had frequent manual handling of heavy loads was 45.5% and those who handled heavy loads in awkward postures accounted for 86.4%. Daily vibration A(8) averaged on the z axis was 0.25 (0.06) m·s -2 and at vector sum was 0.29 (0.07) m·s -2 . Daily vibration exposures on the z axis, frequent manual handling of heavy loads and awkward posture during MMH were significantly associated with LBP. Drivers who are exposed to WBV and frequently handle heavy loads manually and with awkward postures probably have more LBP than drivers who are exposed to only one of these risk factors.

  10. Low back pain in drivers: The relative role of whole-body vibration, posture and manual materials handling

    Science.gov (United States)

    Okunribido, O. O.; Magnusson, M.; Pope, M. H.

    2006-12-01

    A cross-sectional study was conducted to investigate the relative role of whole-body vibration (WBV), posture and manual materials handling (MMH) as risk factors for low back pain (LBP). Using a validated questionnaire, information about health history, posture and MMH performed was obtained from 394 workers who drove vehicles as part of their job (according to seven predefined occupational groups) and 59 who did not. The intention was to reflect a wide range of exposures with the lower end of the exposure spectrum defined as that of non-manual workers who do not drive as part of their job. Based on the questionnaire responses and direct measurements of vibration exposure, personal aggregate measures of exposure were computed for each of the respondents, i.e., total vibration dose (TVD), posture score (PS) and manual handling score (MHS). Odds ratios (and 95% confidence intervals) for back pain were obtained from logistics regression models and log-linear backward elimination analysis was performed. The findings showed that 'combined exposure' due to posture and one or both of vibration and MMH, rather than the individual exposure to one of the three factors (WBV, posture, MMH) is the main contributor of the increased prevalence of LBP.

  11. Resistance training and the enhancement of the gains in material-handling ability and physical fitness of British Army recruits during basic training.

    Science.gov (United States)

    Williams, A G; Rayson, M P; Jones, D A

    2002-03-15

    The aim was to evaluate the efficacy of a modified British Army basic training that included resistance training in improving material-handling performance and physical fitness, and to compare the modified training directly with the normal basic training. Forty-three males [19.2 (2.6) years of age, 1764 (72) mm in height, 73.0 (10.6) kg in mass] and nine females [19.1 (2.2) years, 1641 (67) mm, 62.0 (7.2) kg] performed the modified basic training. Testing occurred in the week before and in the final week of the 11-week basic training. Improvements with the modified training were observed for all six material-handling tests, including 8-12% for maximal box lifting, 15-19% for repetitive lifting and carrying and 9-17% for loaded marching (all p material-handling ability and other aspects of physical fitness brought about in recruits by British Army basic training can be enhanced by the use of a physical training programme that includes a carefully designed resistance training element. Of particular note are the improvements shown in performance on material-handling tasks that require muscular strength, as these represent many of the tasks that soldiers encounter in their military careers.

  12. Material handling performance of patients with chronic low back pain during functional capacity evaluation: a comparison between three countries.

    Science.gov (United States)

    Reneman, M F; Kool, J; Oesch, P; Geertzen, J H B; Battié, M C; Gross, D P

    2006-09-30

    Functional Capacity Evaluations (FCEs) are batteries of tests designed to measure patients' ability to perform work-related activities. Although FCEs are used worldwide, it is unknown how patients' performances compare between countries or settings. This study was performed to explore similarities and differences in FCE performance of patients with chronic low back pain (CLBP) between three international settings that utilize the same FCE protocol. Standardized FCEs were performed on three cohorts of patients with CLBP: A sample from an outpatient rehabilitation context in The Netherlands (n = 121), a Canadian sample in a Worker's Compensation context (n = 273), and a Swiss sample in an inpatient rehabilitation context (n = 170). Patients were undergoing FCE as part of their usual clinical care. Means and standard deviations of maximum performance on the FCE material handling items were calculated and differences compared using ANOVA. Multivariable linear regression was used to determine the relationship between country of origin and FCE performance while controlling for potential confounders including, age, sex, duration of back pain problems, and self-reported pain and disability ratings. Compared to the Dutch sample, the mean performance of patients in the Canadian and Swiss samples was consistently lower on all FCE items. This association remained statistically significant after controlling for potential confounders. Considerable differences were observed between settings in maximum weight handled on the various FCE items. Future FCE research should examine the effects of a number of potentially influential factors, including variability in evaluator judgements across settings, the evaluator-patient interaction and patients' expectations of the influence of FCE results on disability compensation.

  13. Metabolic Signatures of Oxidative Stress and Their Relationship with Erythrocyte Membrane Surface Roughness Among Workers of Manual Materials Handling (MMH).

    Science.gov (United States)

    Ghosh, Subrata; Acharyya, Muktish; Majumder, Titlee; Bagchi, Anandi

    2015-12-01

    Brickfield workers in India perform manual materials handling (MMH) and as a result, are at a high risk of developing oxidative stress. This results in an alteration of the various markers of metabolic oxidative stress at the cellular level. Since red blood cell (RBC) is the central point where oxygen, glucose-6-phosphate dehydrogenase (G-6-PD), and glutathione (GSH) are involved, the surface roughness and its alteration and modeling with respect to workers exposed to MMH may be considered as helpful determinants in predicting early damage to the cell and restoring better health to the exposed population, that is, the worker exposed to stress. Hence, nanometric analysis of the surface roughness of the RBC may serve as an early indicator of the stress-related damage in these individuals. The purpose of the study was to identify early red blood corpuscular surface damage profile in terms of linear modeling correlating various biochemical parameters. Linear modeling has been aimed to be developed in order to demonstrate how individual oxidative stress markers such as malondialdehyde (MDA), G-6-PD, and reduced GSH are related to the RBC surface roughness [root mean square (RMS)]. Conventional analysis of these biochemical responses were evaluated in MMH laborers (age varying between 18 years and 21 years) and a comparable control group of the same age group (with sedentary lifestyles). Peak expiratory flow rate (PEFR) and RBC surface analysis by atomic-force microscopy (AFM) and correlated scanning probe microscopy (SPM-analytical software) with corresponding image analysis were performed immediately after completion of standardized exercise (MMH) at the brickfield. A number of correlated significances and regressive linear models were developed among MDA, G-6-PD, GSH, and RBC surface roughness. It appears that these linear models might be instrumental in predicting early oxidative damages related to specific occupational hazards.

  14. Narrative text analysis of accident reports with tractors, self-propelled harvesting machinery and materials handling machinery in Austrian agriculture from 2008 to 2010 - a comparison.

    Science.gov (United States)

    Mayrhofer, Hannes; Quendler, Elisabeth; Boxberger, Josef

    2014-01-01

    The aim of this study was the identification of accident scenarios and causes by analysing existing accident reports of recognized agricultural occupational accidents with tractors, self-propelled harvesting machinery and materials handling machinery from 2008 to 2010. As a result of a literature-based evaluation of past accident analyses, the narrative text analysis was chosen as an appropriate method. A narrative analysis of the text fields of accident reports that farmers used to report accidents to insurers was conducted to obtain detailed information about the scenarios and causes of accidents. This narrative analysis of reports was made the first time and yielded first insights for identifying antecedents of accidents and potential opportunities for technical based intervention. A literature and internet search was done to discuss and confirm the findings. The narrative text analysis showed that in more than one third of the accidents with tractors and materials handling machinery the vehicle rolled or tipped over. The most relevant accident scenarios with harvesting machinery were being trapped and falling down. The direct comparison of the analysed machinery categories showed that more than 10% of the accidents in each category were caused by technical faults, slippery or muddy terrain and incorrect or inappropriate operation of the vehicle. Accidents with tractors, harvesting machinery and materials handling machinery showed similarities in terms of causes, circumstances and consequences. Certain technical and communicative measures for accident prevention could be used for all three machinery categories. Nevertheless, some individual solutions for accident prevention, which suit each specific machine type, would be necessary.

  15. The role of whole body vibration, posture and manual materials handling as risk factors for low back pain in occupational drivers.

    Science.gov (United States)

    Okunribido, O O; Magnusson, M; Pope, M H

    2008-03-01

    It seems evident that occupational drivers have an increased risk of developing back pain. Not only are they exposed to whole body vibration (vibration), their work often includes exposure to several other risk factors for low back pain (LBP), particularly the seated posture (posture) and manual materials handling (MMH). Excessive demands on posture are likely to be aggravated by vibration and vice versa, and the risks may be further compounded when MMH is performed. This study investigated the relative role of vibration, posture and MMH as risk factors for LBP and the stated hypothesis was that the risks for LBP in drivers are the combined effect of vibration, posture and/or MMH. The findings showed that interaction effects due to posture and one or both of vibration and MMH, rather than the individual exposure effects, are the main contributors for precipitation of LBP.

  16. The basic characteristics of hybrid aircraft. [structural design criteria and weight analysis of airships for materials handling

    Science.gov (United States)

    Nichols, J. B.

    1975-01-01

    The transportation of very heavy or very bulky loads by airships, and the ability to carry out extended duration flights at low speeds and low costs was studied. Structural design and weight factors for airship construction are examined. The densities of various light gases to be used in airships are given, along with their lifting capacities. The aerodynamic characteristics of various airship configurations was studied. Propulsion system requirements for airships are briefly considered.

  17. Relative importance of expertise, lifting height and weight lifted on posture and lumbar external loading during a transfer task in manual material handling.

    Science.gov (United States)

    Plamondon, André; Larivière, Christian; Delisle, Alain; Denis, Denys; Gagnon, Denis

    2012-01-01

    The objective of this study was to measure the effect size of three important factors in manual material handling, namely expertise, lifting height and weight lifted. The effect of expertise was evaluated by contrasting 15 expert and 15 novice handlers, the effect of the weight lifted with a 15-kg box and a 23-kg box and the effect of lifting height with two different box heights: ground level and a 32 cm height. The task consisted of transferring a series of boxes from a conveyor to a hand trolley. Lifting height and weight lifted had more effect size than expertise on external back loading variables (moments) while expertise had low impact. On the other hand, expertise showed a significant effect of posture variables on the lumbar spine and knees. All three factors are important, but for a reduction of external back loading, the focus should be on the lifting height and weight lifted. The objective was to measure the effect size of three important factors in a transfer of boxes from a conveyor to a hand trolley. Lifting height and weight lifted had more effect size than expertise on external back loading variables but expertise was a major determinant in back posture.

  18. French good practice guidelines for management of the risk of low back pain among workers exposed to manual material handling: Hierarchical strategy of risk assessment of work situations.

    Science.gov (United States)

    Petit, Audrey; Mairiaux, Philippe; Desarmenien, Arnaud; Meyer, Jean-Pierre; Roquelaure, Yves

    2016-02-15

    Manual material handling remains a major cause of occupational accidents and diseases in various sectors and occupations. This paper summarizes the main recommendations of the good practice guidelines of the French Society of Occupational Medicine for the risk assessment for back disorders in workers exposed to manual handling of loads. The guidelines were written by a multidisciplinary working group of 24 experts, according to the Clinical Practice Guidelines method proposed by French National Health Authority, and reviewed by a multidisciplinary peer review committee of 50 experts. Recommendations were based on a large systematic review of the international literature carried out from 1990 to March 2012 and classified (Grade A, B, C or expert consensus) according to their level of evidence. The main recommendations are a three-level hierarchical method of risk assessment based on participatory ergonomics and suggested assessment tools that can be used routinely by professionals of occupational health, workers themselves and their supervisors. These French guidelines are intended for professionals of occupational health in charge of the prevention of low back disorders. The recommended methods are applicable to other countries than France.

  19. Effects of different lifting and lowering heights on upper arm, shoulder and back muscle activity during a manual material handling task.

    Science.gov (United States)

    Yoo, In-gyu; Yoo, Won-gyu

    2015-01-01

    Previous research has suggested eliminating certain types of manual material handling (MMH) work by recommending specific arm angles and postures to avoid, such as arm flexion or abduction over 90°. MMH with arm flexion over 90° can require lifting objects as well as lowering objects. However, few studies have evaluated MMH work while lowering objects in detail. This study investigated the effects of different lifting and lowering heights on upper arm, shoulder, and back muscle activity during a MMH task. The participants performed a MMH task that involved stoop lowering and lifting. The participants transferred the box to shelves positioned 30  cm in front of them under various conditions. Conditions 1 to 4 involved transferring the box to 1) ankle-, 2) knee-, 3) waist-, and 4) shoulder-high shelves, respectively. Surface electrodes were attached to the biceps brachii, upper trapezius, rhomboid minor, and L4 erector spinae. The activity of the biceps brachii was decreased significantly in Conditions 1 and 2 compared to Conditions 3 and 4. The upper trapezius activity was increased significantly in Conditions 1 and 4 compared to Conditions 2 and 3. The rhomboid minor activity increased significantly in Condition 1 compared to Conditions 2 to 4. The L4 erector spinae activity decreased significantly in Condition 1 compared to Conditions 2 to 4 CONCLUSIONS:A low-lowering MMH work could contribute to neck, shoulder, and back pain. Therefore, further studies must examine a height below-knee MMH work in detail.

  20. The effect of wearing a lumbar belt on biomechanical and psychological outcomes related to maximal flexion-extension motion and manual material handling.

    Science.gov (United States)

    Shahvarpour, Ali; Preuss, Richard; Sullivan, Michael J L; Negrini, Alessia; Larivière, Christian

    2018-05-01

    Workers with low back pain (LBP) may benefit from wearing a lumbar belt (LB), but the biomechanical and psychological mechanisms involved are not fully understood. Two types of flexible LB (extensible and non-extensible) were compared to a control condition (no LB) regarding pain-related (pain, fear of pain and catastrophizing) and biomechanical (range of motion - ROM) outcomes related to two tasks: maximal trunk flexion-extension and manual material handling. Healthy controls and participants with LBP were tested. During both tasks, the two LBs reduced the lumbar ROM in participants with LBP in the same way as healthy controls. This was observed even at the beginning of the trunk flexion movement, allowing generalization to many work tasks, that is to say tasks performed with small or deep trunk flexion. The two LBs reduced pain, fear of pain and catastrophizing in subjects with LBP. That may help a gradual re-exposure to physical work activities (disability prevention perspective), or maintaining these activities (secondary prevention perspective), following a LBP episode. Copyright © 2018. Published by Elsevier Ltd.

  1. G-6-PD level and surface nanoscopy: a novel approach in ergonomic stress management of female labours in Bengal suburbs performing manual material handling.

    Science.gov (United States)

    Ghosh, Subrata; Acharyya, Muktish; Bagchi, Anandi

    2009-12-01

    Strenous physical exercise like professional load bearing often produces oxidative stress, increasing post exercise Malondialdehyde (MDA) levels. To quantify the cellular dimension/profile of the said stress, nanoscopic observation of the erythrocyte surface was made by Atomic Forced Microscopy (AFM)/Lateral Forced Microscopy (LFM) and correspondingly the average roughness of the surface was measured. An attempt has been made to correlate the antioxidant vitamin mixture supplementation, endurance capacity, allied physiological parameters and blood glucose-6-phosphate dehydrogenase (G-6-PD) level and roughness-MDA correlation and thereby the deduced regression equation as crucial markers of performance related oxidative stress management in professional female load bearers. Three experimental groups A, B and placebo, each consisting of ten female workers (18-21 years old), were chosen. Group A was given 400 mg of vitamin E supplementation daily, while Group B was given a clinical mixture of vitamin E, vitamin C and beta-carotene daily in capsular form for a period of 28 days. The exercise- induced hike in the status of serum MDA was found to rise less significantly with vitamin supplementation. Further study showed that the supplementation was instrumental in reducing the basal MDA level. Endurance capacity, determined by bicycle ergometric method, was increased more significantly (p material handling.

  2. The assessment of material-handling strategies in dealing with sudden loading: the effect of uneven ground surface on trunk biomechanical responses.

    Science.gov (United States)

    Zhou, Jie; Ning, Xiaopeng; Nimbarte, Ashish D; Dai, Fei

    2015-01-01

    As a major risk factor of low back injury, sudden loading often occurs when performing manual material-handling tasks on uneven ground surfaces. Therefore, the purpose of the current study was to investigate the effects of a laterally slanted ground on trunk biomechanical responses during sudden loading events. Thirteen male subjects were subjected to suddenly released loads of 3.4 and 6.8 kg, while standing on a laterally slanted ground of 0°, 15° and 30°. The results showed that 8.3% and 5.6% larger peak L5/S1 joint compression forces were generated in the 30° condition compared with the 0° and 15° conditions, respectively. The increase of L5/S1 joint moment in the 30° condition was 8.5% and 5.0% greater than the 0° and 15° conditions, respectively. Findings of this study suggest that standing on a laterally slanted ground could increase mechanical loading on the spine when experiencing sudden loading. Practitioner Summary: Sudden loading is closely related to occupational low back injuries. The results of this study showed that the increase of slanted ground angle and magnitude of load significantly increase the mechanical loading on the spine during sudden loading. Therefore, both of these two components should be controlled in task design.

  3. Factors affecting the perception of whole-body vibration of occupational drivers: an analysis of posture and manual materials handling and musculoskeletal disorders.

    Science.gov (United States)

    Raffler, Nastaran; Ellegast, Rolf; Kraus, Thomas; Ochsmann, Elke

    2016-01-01

    Due to the high cost of conducting field measurements, questionnaires are usually preferred for the assessment of physical workloads and musculoskeletal disorders (MSDs). This study compares the physical workloads of whole-body vibration (WBV) and awkward postures by direct field measurements and self-reported data of 45 occupational drivers. Manual materials handling (MMH) and MSDs were also investigated to analyse their effect on drivers' perception. Although the measured values for WBV exposure were very similarly distributed among the drivers, the subjects' perception differed significantly. Concerning posture, subjects seemed to estimate much better when the difference in exposure was significantly large. The percentage of measured awkward trunk and head inclination were significantly higher for WBV-overestimating subjects than non-overestimators; 77 and 80% vs. 36 and 33%. Health complaints in terms of thoracic spine, cervical spine and shoulder-arm were also significantly more reported by WBV-overestimating subjects (42, 67, 50% vs. 0, 25, 13%, respectively). Although more MMH was reported by WBV-overestimating subjects, there was no statistical significance in this study. Self-reported exposures of occupational drivers are affected by many other cofactors, and this can result in misinterpretations. A comparison between field measurement and questionnaire was used to highlight the factors affecting the perception of drivers for whole-body vibration (WBV) exposure. Posture and musculoskeletal disorders influenced the perception of the similarly WBV-exposed drivers significantly.

  4. City bus driving and low back pain: a study of the exposures to posture demands, manual materials handling and whole-body vibration.

    Science.gov (United States)

    Okunribido, Olanrewaju O; Shimbles, Steven J; Magnusson, Marianne; Pope, Malcolm

    2007-01-01

    A cross-sectional study was conducted to investigate worker exposure to posture demands, manual materials handling (MMH) and whole body vibration as risks for low back pain (LBP). Using validated questionnaire, information about driving experience, driving (sitting) posture MMH, and health history was obtained from 80 city bus drivers. Twelve drivers were observed during their service route driving (at least one complete round trip) and vibration measurements were obtained at the seat and according to the recommendations of ISO 2631 (1997), for three models of bus (a mini-bus, a single-decker bus, a double-decker bus). The results showed that city bus drivers spend about 60% of the daily work time actually driving, often with the torso straight or unsupported, perform occasional and light MMH, and experience discomforting shock/jerking vibration events. Transient and mild LBP (not likely to interfere with work or customary levels of activity) was found to be prevalent among the drivers and a need for ergonomic evaluation of the drivers' seat was suggested.

  5. Signal transduction in cells of the immune system in microgravity

    Directory of Open Access Journals (Sweden)

    Huber Kathrin

    2008-10-01

    Full Text Available Abstract Life on Earth developed in the presence and under the constant influence of gravity. Gravity has been present during the entire evolution, from the first organic molecule to mammals and humans. Modern research revealed clearly that gravity is important, probably indispensable for the function of living systems, from unicellular organisms to men. Thus, gravity research is no more or less a fundamental question about the conditions of life on Earth. Since the first space missions and supported thereafter by a multitude of space and ground-based experiments, it is well known that immune cell function is severely suppressed in microgravity, which renders the cells of the immune system an ideal model organism to investigate the influence of gravity on the cellular and molecular level. Here we review the current knowledge about the question, if and how cellular signal transduction depends on the existence of gravity, with special focus on cells of the immune system. Since immune cell function is fundamental to keep the organism under imnological surveillance during the defence against pathogens, to investigate the effects and possible molecular mechanisms of altered gravity is indispensable for long-term space flights to Earth Moon or Mars. Thus, understanding the impact of gravity on cellular functions on Earth will provide not only important informations about the development of life on Earth, but also for therapeutic and preventive strategies to cope successfully with medical problems during space exploration.

  6. The design of in-cell crane handling systems for nuclear plants

    International Nuclear Information System (INIS)

    Hansford, S.M.; Scott, R.

    1992-01-01

    The reprocessing and waste management facilities at (BNFL's) British Nuclear Fuels Limited's Sellafield site make extensive use of crane handling systems. These range from conventional mechanical handling operations as used generally in industry to high integrity applications through to remote robotic handling operations in radiation environments. This paper describes the design methodologies developed for the design of crane systems for remote handling operations - in-cell crane systems. In most applications the in-cell crane systems are an integral part of the plant process equipment and reliable and safe operations are a key design parameter. Outlined are the techniques developed to achieve high levels of crane system availability for operations in hazardous radiation environments. These techniques are now well established and proven through many years of successful plant operation. A recent application of in-cell crane handling systems design for process duty application is described. The benefits of a systematic design approach and a functionally-based engineering organization are also highlighted. (author)

  7. First experience with particle-in-cell plasma physics code on ARM-based HPC systems

    Science.gov (United States)

    Sáez, Xavier; Soba, Alejandro; Sánchez, Edilberto; Mantsinen, Mervi; Mateo, Sergi; Cela, José M.; Castejón, Francisco

    2015-09-01

    In this work, we will explore the feasibility of porting a Particle-in-cell code (EUTERPE) to an ARM multi-core platform from the Mont-Blanc project. The used prototype is based on a system-on-chip Samsung Exynos 5 with an integrated GPU. It is the first prototype that could be used for High-Performance Computing (HPC), since it supports double precision and parallel programming languages.

  8. Modification of antioxidant systems in cell walls of maize roots by different nitrogen sources

    Energy Technology Data Exchange (ETDEWEB)

    Hadži-Tašković Šukalović V; Vuletić, M.; Marković, K.; Željko, Vučinić; Kravić, N.

    2016-07-01

    Antioxidant systems of maize root cell walls grown on different nitrogen sources were evaluated. Plants were grown on a medium containing only NO3- or the mixture of NO3-+NH4+, in a 2:1 ratio. Eleven-day old plants, two days after the initiation of lateral roots, were used for the experiments. Cell walls were isolated from lateral roots and primary root segments, 2-7 cm from tip to base, representing zones of intense or decreased growth rates, respectively. Protein content and the activity of enzymes peroxidase, malate dehydrogenase and ascorbate oxidase ionically or covalently bound to the walls, as well as cell wall phenolic content and antioxidant capacity, were determined. Cell walls of plants grown on mixed N possess more developed enzymatic antioxidant systems and lower non-enzymatic antioxidant defenses than cell walls grown on NO3-. Irrespective of N treatment, the activities of all studied enzymes and protein content were higher in cell walls of lateral compared to primary roots. Phenolic content of cell walls isolated from lateral roots was higher in NO3--grown than in mixed N grown plants. No significant differences could be observed in the isozyme patterns of cell wall peroxidases isolated from plants grown on different nutrient solution. Our results indicate that different N treatments modify the antioxidant systems of root cell walls. Treatment with NO3- resulted in an increase of constitutive phenolic content, while the combination of NO3-+NH4+ elevated the redox enzyme activities in root cell walls.

  9. Materials Handling for Urban Tunneling in Rock

    Science.gov (United States)

    1979-01-01

    An examination of prior forecasts of tunnel construction provides an estimate of 2.4 million feet of rock tunnel to be constructed during the 1976-2000 period. Tunnel projects for the near term (1980+) and far term (1990+) periods are defined for stu...

  10. ANALISIS MANUAL MATERIAL HANDLING MENGGUNAKAN NIOSH EQUATION

    Directory of Open Access Journals (Sweden)

    Etika Muslimah

    2006-12-01

    Full Text Available Aktivitas penanganan materal yang tidak tepat dapat mengakibatkan kerugian dan kecelakaan pada operator. Salah satunya adalah cedera pada sistem muskuloskeletal atau muskoloskeletal disorder (MSDs. Makalah ini mengkaji aktivitas penanganan material pada pekerja panggul beras pada gudang gudang BULOG Sub Depot Logistic Wil III Surakarta Pergudangan Beras 305 Grogol, Sukoharjo, dengan menggunakan NIOSH equation. Dari hasil pengukuran terhadap 4 pekerja yang melakukan kegiatan panggul beras, diperoleh bahwa kegiatan ini termasuk kegiatan yang ringan berdasarkan perhitungan konsumsi energi. Namun demikian, beban angkat aktual diatas beban yang direkomendasikan dengan tingkat Lifting Index (LI > 1, dan berpotensi menyebabkan MSDs.

  11. Manual materials handling in simulated motion environments.

    Science.gov (United States)

    Holmes, Michael W; MacKinnon, Scott N; Matthews, Julie; Albert, Wayne J; Mills, Steven

    2008-05-01

    Seafaring occupations have been shown to place operators at an increased risk for injury. The purpose of this study was to understand better the demands of a moving environment on the ability of a person to perform specific lifting tasks. Subjects lifted a 15-kg load under four different lifting conditions. A 6-degree-of-freedom ship motion simulator imposed repeatable deck motions under foot while subjects executed the lifting tasks. Subjects were oriented in three different positions on the simulator floor to inflict different motion profiles. Electromyographic records of four muscles were collected bilaterally, and thoracolumbar kinematics were measured. A repeated-measures ANOVA was employed to assess trunk motions and muscle activities across lifting and motion conditions. The erector spinae muscles showed a trend toward significant differences for motion effects. Maximal sagittal velocities were significantly smaller for all motion states in comparison with the stable condition (p

  12. Material Handling Equipment Evaluation for Crater Repair

    Science.gov (United States)

    2016-11-01

    load concrete repair materials and accelerate other portions of the airfield damage repair process. Integrated lifting mechanisms utilized in other...Specifically identified were front-lifting hydraulic arms (see Figure 21) commonly observed to empty steel refuse collection containers. Available...sacks and/or the integrated lifting arms would require extensive modification for operational efficiency. • The current operational procedures would

  13. New bioproduction systems: from molecular circuits to novel reactor concepts in cell-free biotechnology.

    Science.gov (United States)

    Rupp, Steffen

    2013-01-01

    : The last decades witnessed a strong growth in several areas of biotechnology, especially in fields related to health, as well as in industrial biotechnology. Advances in molecular engineering now enable biotechnologists to design more efficient pathways in order to convert a larger spectrum of renewable resources into industrially used biofuels and chemicals as well as into new pharmaceuticals and therapeutic proteins. In addition material sciences advanced significantly making it more and more possible to integrate biology and engineering. One of the key questions currently is how to develop new ways of engineering biological systems to cope with the complexity and limitations given by the cell. The options to integrate biology with classical engineering advanced cell free technologies in the recent years significantly. Cell free protein production using cellular extracts is now a well-established universal technology for production of proteins derived from many organisms even at the milligram scale. Among other applications it has the potential to supply the demand for a multitude of enzymes and enzyme variants facilitating in vitro metabolic engineering. This review will briefly address the recent achievements and limitations of cell free conversions. Especially, the requirements for reactor systems in cell free biotechnology, a currently underdeveloped field, are reviewed and some perspectives are given on how material sciences and biotechnology might be able to advance these new developments in the future.

  14. Particle-in-Cell Simulations of the VENUS Ion Beam Transport System

    CERN Document Server

    Todd, Damon; Leitner, Daniela; Lyneis, Claude; Qiang, Ji

    2005-01-01

    The next-generation superconducting ECR ion source VENUS serves as the prototype injector ion source for the linac driver of the proposed Rare Isotope Accelerator (RIA). The high-intensity heavy ion beams required by the RIA driver linac present significant challenges for the design and simulation of an ECR extraction and low energy ion beam transport system. Extraction and beam formation take place in a strong (up to 3T) axial magnetic field, which leads to significantly different focusing properties for the different ion masses and charge states of the extracted beam. Typically, beam simulations must take into account the contributions of up to 30 different charge states and ion masses. Two three-dimensional, particle-in-cell codes developed for other purposes, IMPACT and WARP, have been adapted in order to model intense, multi-species DC beams. A discussion of the differences of these codes and the advantages of each in the simulation of the low energy beam transport system of an ECR ion source is given. D...

  15. The development of remote repairing system, decontamination and in-cell remote inspection equipment

    International Nuclear Information System (INIS)

    Ishibashi, Yuzo; Toyoda, Osamu; Haginoya, Isao; Yamamoto, Ryuichi; Tanaka, Yasumasa

    1993-01-01

    PNC has been developing remote repair and inspection technologies for in-cell components in reprocessing Plants. In this report, several remote technologies such as remote dismantling and removal, decontamination, remote pipe maintenance and remote in-cell inspection equipment are described. (author)

  16. Rule-based modeling: a computational approach for studying biomolecular site dynamics in cell signaling systems.

    Science.gov (United States)

    Chylek, Lily A; Harris, Leonard A; Tung, Chang-Shung; Faeder, James R; Lopez, Carlos F; Hlavacek, William S

    2014-01-01

    Rule-based modeling was developed to address the limitations of traditional approaches for modeling chemical kinetics in cell signaling systems. These systems consist of multiple interacting biomolecules (e.g., proteins), which themselves consist of multiple parts (e.g., domains, linear motifs, and sites of phosphorylation). Consequently, biomolecules that mediate information processing generally have the potential to interact in multiple ways, with the number of possible complexes and posttranslational modification states tending to grow exponentially with the number of binary interactions considered. As a result, only large reaction networks capture all possible consequences of the molecular interactions that occur in a cell signaling system, which is problematic because traditional modeling approaches for chemical kinetics (e.g., ordinary differential equations) require explicit network specification. This problem is circumvented through representation of interactions in terms of local rules. With this approach, network specification is implicit and model specification is concise. Concise representation results in a coarse graining of chemical kinetics, which is introduced because all reactions implied by a rule inherit the rate law associated with that rule. Coarse graining can be appropriate if interactions are modular, and the coarseness of a model can be adjusted as needed. Rules can be specified using specialized model-specification languages, and recently developed tools designed for specification of rule-based models allow one to leverage powerful software engineering capabilities. A rule-based model comprises a set of rules, which can be processed by general-purpose simulation and analysis tools to achieve different objectives (e.g., to perform either a deterministic or stochastic simulation). © 2013 Wiley Periodicals, Inc.

  17. Radioactive waste multiple container system

    International Nuclear Information System (INIS)

    Smith, R.J.; Garman, L.D.; Reynolds, W.E.

    1984-01-01

    A radioactive waste multiple container system is described which provides a simplified method whereby a plurality of drums can be placed in a remote, unmanned storage facility in one materials handling sequence, as opposed to a plurality of separate materials handling sequences, such as required for individual drums utilizing a remotely-controlled materials handling system. The multiple container system also functions as an interim storage system in that it will allow for stacking up to a total of ten levels high. Each container interlocks with each successive unit, through automatic, passive alignment of the individual container with the previously placed unit, while being handled completely by remote control. This interlock and alignment feature insures greater stack stability, substantially increasing the overall storage efficiency of the remote storage facility. While a '6-pack' container system has been described, a 4, 8, 10, 12, etc. system may be used instead. (author)

  18. Overhead remote handling systems for the process facility modifications project

    International Nuclear Information System (INIS)

    Wiesener, R.W.; Grover, D.L.

    1987-01-01

    Each of the cells in the process facility modifications (PFM) project complex is provided with a variety of general purpose remote handling equipment including bridge cranes, monorail hoist, bridge-mounted electromechanical manipulator (EMM) and an overhead robot used for high efficiency particulate air (HEPA) filter changeout. This equipment supplements master-slave manipulators (MSMs) located throughout the complex to provide an overall remote handling system capability. The overhead handling equipment is used for fuel and waste material handling operations throughout the process cells. The system also provides the capability for remote replacement of all in-cell process equipment which may fail or be replaced for upgrading during the lifetime of the facility

  19. The role of protective systems in cell homeostasis upon gamma radiation

    International Nuclear Information System (INIS)

    Zasuxina, Q.D.

    2002-01-01

    -treatment. Probably it is related to high activity of SOD in children after chronic radiation. Similar data about increased activity of SOD were obtained too by Durnov et al. (2000). We received unexpected results investigating the effect of trien on RAR formation. First at all we didn't find any influence of trien on the level of RAR: the level of RAR in cells was similar without and with trien-treatment. Secondly, RAR did not distinguish in repair-deficient cells in comparison with results for healthy donors. So we received the proofs that SOD didn't take part in the formation of RAR. Next part of our investigation were devoted to the comparison of trien effect and garlic extract (GE)-pretreatment on the viability of human cells after gamma-radiation and during RAR. GE decreased the number of killed cells, trien-increased this number. However trien didn't affect on the cell survival during RAR. Several mechanisms have been proposed to explain the mutagen-preventive effects of GE. These include modulation of enzyme activity, inhibition of DNA adduct formation, free-radical scavenging and effects on cell proliferation. GE can inhibit the nitrosation reaction in vitro, also decreased DNA methylation and the formation O6-G induced by some mutagens. GE stimulated the activity of glutathion peroxidase and enzymes involved in natural protection by free radicals in addition to SOD. The activity of SOD also increased by GE. In conclusion it seems that inhibition of SOD increases the cell sensibility to gamma-radiation. Inhibition of SOD does not affect the number of DNA breaks in lymphocytes of children living in regions with an increased level of radiation. The level of cell defence against gamma radiation is similar in the experiments with GE-pretreatment and during RAR

  20. Explicit high-order non-canonical symplectic particle-in-cell algorithms for Vlasov-Maxwell systems

    Energy Technology Data Exchange (ETDEWEB)

    Xiao, Jianyuan [School of Nuclear Science and Technology and Department of Modern Physics, University of Science and Technology of China, Hefei, Anhui 230026, China; Key Laboratory of Geospace Environment, CAS, Hefei, Anhui 230026, China; Qin, Hong [School of Nuclear Science and Technology and Department of Modern Physics, University of Science and Technology of China, Hefei, Anhui 230026, China; Plasma Physics Laboratory, Princeton University, Princeton, New Jersey 08543, USA; Liu, Jian [School of Nuclear Science and Technology and Department of Modern Physics, University of Science and Technology of China, Hefei, Anhui 230026, China; Key Laboratory of Geospace Environment, CAS, Hefei, Anhui 230026, China; He, Yang [School of Nuclear Science and Technology and Department of Modern Physics, University of Science and Technology of China, Hefei, Anhui 230026, China; Key Laboratory of Geospace Environment, CAS, Hefei, Anhui 230026, China; Zhang, Ruili [School of Nuclear Science and Technology and Department of Modern Physics, University of Science and Technology of China, Hefei, Anhui 230026, China; Key Laboratory of Geospace Environment, CAS, Hefei, Anhui 230026, China; Sun, Yajuan [LSEC, Academy of Mathematics and Systems Science, Chinese Academy of Sciences, P.O. Box 2719, Beijing 100190, China

    2015-11-01

    Explicit high-order non-canonical symplectic particle-in-cell algorithms for classical particle-field systems governed by the Vlasov-Maxwell equations are developed. The algorithms conserve a discrete non-canonical symplectic structure derived from the Lagrangian of the particle-field system, which is naturally discrete in particles. The electromagnetic field is spatially discretized using the method of discrete exterior calculus with high-order interpolating differential forms for a cubic grid. The resulting time-domain Lagrangian assumes a non-canonical symplectic structure. It is also gauge invariant and conserves charge. The system is then solved using a structure-preserving splitting method discovered by He et al. [preprint arXiv: 1505.06076 (2015)], which produces five exactly soluble sub-systems, and high-order structure-preserving algorithms follow by combinations. The explicit, high-order, and conservative nature of the algorithms is especially suitable for long-term simulations of particle-field systems with extremely large number of degrees of freedom on massively parallel supercomputers. The algorithms have been tested and verified by the two physics problems, i.e., the nonlinear Landau damping and the electron Bernstein wave. (C) 2015 AIP Publishing LLC.

  1. Explicit high-order non-canonical symplectic particle-in-cell algorithms for Vlasov-Maxwell systems

    International Nuclear Information System (INIS)

    Xiao, Jianyuan; Liu, Jian; He, Yang; Zhang, Ruili; Qin, Hong; Sun, Yajuan

    2015-01-01

    Explicit high-order non-canonical symplectic particle-in-cell algorithms for classical particle-field systems governed by the Vlasov-Maxwell equations are developed. The algorithms conserve a discrete non-canonical symplectic structure derived from the Lagrangian of the particle-field system, which is naturally discrete in particles. The electromagnetic field is spatially discretized using the method of discrete exterior calculus with high-order interpolating differential forms for a cubic grid. The resulting time-domain Lagrangian assumes a non-canonical symplectic structure. It is also gauge invariant and conserves charge. The system is then solved using a structure-preserving splitting method discovered by He et al. [preprint http://arxiv.org/abs/arXiv:1505.06076 (2015)], which produces five exactly soluble sub-systems, and high-order structure-preserving algorithms follow by combinations. The explicit, high-order, and conservative nature of the algorithms is especially suitable for long-term simulations of particle-field systems with extremely large number of degrees of freedom on massively parallel supercomputers. The algorithms have been tested and verified by the two physics problems, i.e., the nonlinear Landau damping and the electron Bernstein wave

  2. Screening system of blocking agents of the receptor for advanced glycation endproducts in cells using fluorescence.

    Science.gov (United States)

    Jung, Dong Ho; Kim, Young Sook; Kim, Jin Sook

    2012-01-01

    Activation of the receptor for advanced glycation endproducts (RAGE) triggers cellular responses implicated in the pathogenesis of diabetic complications; blockade of RAGE has been shown to inhibit the development of diabetic complications. To develop a screening system to identify novel disruptors of advanced glycation endproducts (AGE)-RAGE binding, we used an AGE-RAGE binding system in RAGE-overexpressing cells; test compounds were screened using this system. To construct human RAGE-overexpressing cells, mouse mesangial cells (MMCs) were stably transfected with the pcDNA-human RAGE (hRAGE) vector and selected under 1 mg/mL gentamicin (G418). RAGE expression in hRAGE-overexpressing MMCs was analyzed by Western blotting with specific RAGE antibody. To identify novel disruptors of AGE-RAGE binding, 50 single compounds and AGE-bovine serum albumin (BSA)-Alexa 488 (AGE-BSA labeled with Alexa 488) were treated to the hRAGE-overexpressing MMCs. Nonbinding AGE-BSA-Alexa 488 was washed and fluorescence measured by microtiter plate reader (excitation wavelength, 485 nm; emission wavelength, 528 nm). In hRAGE-overexpressing cells, only treatment with AGE-BSA-Alexa 488 significantly increased fluorescence intensity in a dose-dependent manner. Of 50 compounds tested, genistein disrupted AGE-RAGE binding in a dose-dependent manner. This AGE-RAGE binding system using AGE-BSA-Alexa 488 in hRAGE-overexpressing cells was suitable for screening of agents that disrupt AGE-hRAGE binding.

  3. The good of two worlds: increasing complexity in cell-free systems.

    Science.gov (United States)

    Billerbeck, Sonja; Härle, Johannes; Panke, Sven

    2013-12-01

    In vitro biocatalytic systems have moved far beyond established uses in food, diagnostic, and chemical applications. As new strategies to construct and manage multiple enzymes in ever more complex systems are developed, novel applications emerge. In the field of chemistry, complex protein networks are applied to enable the production of fine chemicals, such as dihydroxyacetone phosphate, and even bulk chemicals, such as biofuels, from cheap sugars. Cell-free protein synthesis is applied to expanding protein and nucleic acid biochemistry and enabling novel assay formats, while programmable DNA-circuits can be exploited to engineer sensitive detection methods. Novel developments in chemical analytics such as real-time mass spectrometry to follow the metabolism online, directed physical assembly of network members facilitating substrate channeling, and encapsulation forming biofunctional subunits enable a better control and potential for optimization. Copyright © 2013 Elsevier Ltd. All rights reserved.

  4. Changes in cell adhesion molecule expression on T cells associated with systemic virus infection

    DEFF Research Database (Denmark)

    Andersson, E C; Christensen, Jan Pravsgaard; Marker, O

    1994-01-01

    analyses showed that T cells with a changed adhesion molecule profile tended to present other cell surface markers indicating a state of cellular activation, e.g., IL-2R, and included all virus-specific CTL effectors. Regarding the physiologic significance of these changes in adhesion molecule expression......Virus-induced changes in adhesion molecule expression on T cells were investigated to understand how antiviral effector cells migrate into infectious foci. FACS analysis revealed that after systemic infection with lymphocytic choriomeningitis virus a number of cell adhesion molecules, including VLA...

  5. Changes in cell adhesion molecule expression on T cells associated with systemic virus infection

    DEFF Research Database (Denmark)

    Andersson, E C; Christensen, Jan Pravsgaard; Marker, O

    1994-01-01

    Virus-induced changes in adhesion molecule expression on T cells were investigated to understand how antiviral effector cells migrate into infectious foci. FACS analysis revealed that after systemic infection with lymphocytic choriomeningitis virus a number of cell adhesion molecules, including VLA...... analyses showed that T cells with a changed adhesion molecule profile tended to present other cell surface markers indicating a state of cellular activation, e.g., IL-2R, and included all virus-specific CTL effectors. Regarding the physiologic significance of these changes in adhesion molecule expression...

  6. Reverse Genetics System Demonstrates that Rotavirus Nonstructural Protein NSP6 Is Not Essential for Viral Replication in Cell Culture.

    Science.gov (United States)

    Komoto, Satoshi; Kanai, Yuta; Fukuda, Saori; Kugita, Masanori; Kawagishi, Takahiro; Ito, Naoto; Sugiyama, Makoto; Matsuura, Yoshiharu; Kobayashi, Takeshi; Taniguchi, Koki

    2017-11-01

    The use of overlapping open reading frames (ORFs) to synthesize more than one unique protein from a single mRNA has been described for several viruses. Segment 11 of the rotavirus genome encodes two nonstructural proteins, NSP5 and NSP6. The NSP6 ORF is present in the vast majority of rotavirus strains, and therefore the NSP6 protein would be expected to have a function in viral replication. However, there is no direct evidence of its function or requirement in the viral replication cycle yet. Here, taking advantage of a recently established plasmid-only-based reverse genetics system that allows rescue of recombinant rotaviruses entirely from cloned cDNAs, we generated NSP6-deficient viruses to directly address its significance in the viral replication cycle. Viable recombinant NSP6-deficient viruses could be engineered. Single-step growth curves and plaque formation of the NSP6-deficient viruses confirmed that NSP6 expression is of limited significance for RVA replication in cell culture, although the NSP6 protein seemed to promote efficient virus growth. IMPORTANCE Rotavirus is one of the most important pathogens of severe diarrhea in young children worldwide. The rotavirus genome, consisting of 11 segments of double-stranded RNA, encodes six structural proteins (VP1 to VP4, VP6, and VP7) and six nonstructural proteins (NSP1 to NSP6). Although specific functions have been ascribed to each of the 12 viral proteins, the role of NSP6 in the viral replication cycle remains unknown. In this study, we demonstrated that the NSP6 protein is not essential for viral replication in cell culture by using a recently developed plasmid-only-based reverse genetics system. This reverse genetics approach will be successfully applied to answer questions of great interest regarding the roles of rotaviral proteins in replication and pathogenicity, which can hardly be addressed by conventional approaches. Copyright © 2017 American Society for Microbiology.

  7. [Metabolic changes in cells under electromagnetic radiation of mobile communication systems].

    Science.gov (United States)

    Iakimenko, I L; Sidorik, E P; Tsybulin, A S

    2011-01-01

    Review is devoted to the analysis of biological effects of microwaves. The results of last years' researches indicated the potential risks of long-term low-level microwaves exposure for human health. The analysis of metabolic changes in living cells under the exposure of microwaves from mobile communication systems indicates that this factor is stressful for cells. Among the reproducible effects of low-level microwave radiation are overexpression of heat shock proteins, an increase of reactive oxygen species level, an increase of intracellular Ca2+, damage of DNA, inhibition of DNA reparation, and induction of apoptosis. Extracellular-signal-regulated kinases ERK and stress-related kinases p38MAPK are involved in metabolic changes. Analysis of current data suggests that the concept of exceptionally thermal mechanism of biological effects of microwaves is not correct. In turn, this raises the question of the need to revaluation of modern electromagnetic standards based on thermal effects of non-ionizing radiation on biological systems.

  8. Gap junctions in cells of the immune system: structure, regulation and possible functional roles

    Directory of Open Access Journals (Sweden)

    J.C. Sáez

    2000-04-01

    Full Text Available Gap junction channels are sites of cytoplasmic communication between contacting cells. In vertebrates, they consist of protein subunits denoted connexins (Cxs which are encoded by a gene family. According to their Cx composition, gap junction channels show different gating and permeability properties that define which ions and small molecules permeate them. Differences in Cx primary sequences suggest that channels composed of different Cxs are regulated differentially by intracellular pathways under specific physiological conditions. Functional roles of gap junction channels could be defined by the relative importance of permeant substances, resulting in coordination of electrical and/or metabolic cellular responses. Cells of the native and specific immune systems establish transient homo- and heterocellular contacts at various steps of the immune response. Morphological and functional studies reported during the last three decades have revealed that many intercellular contacts between cells in the immune response present gap junctions or "gap junction-like" structures. Partial characterization of the molecular composition of some of these plasma membrane structures and regulatory mechanisms that control them have been published recently. Studies designed to elucidate their physiological roles suggest that they might permit coordination of cellular events which favor the effective and timely response of the immune system.

  9. Comparison of particle-in-cell simulation with experiment for thetransport system of the superconducting electron cyclotron resonance ionsource VENUS

    Energy Technology Data Exchange (ETDEWEB)

    Todd, DamonS.; Leitner, Daniela; Leitner, Matthaeus; Lyneis,Claude M.; Qiang, Ji; Grote, Dave P.

    2005-09-19

    The three-dimensional, particle-in-cell code WARP has been enhanced to allow end-to-end beam dynamics simulations of the VENUS beam transport system from the extraction region, through a mass-analyzing magnet, and up to a two-axis emittance scanner. This paper presents first results of comparisons between simulation and experimental data. A helium beam (He+, He2+) is chosen as an initial comparison beam due to its simple mass spectrum. Although a number of simplifications are made for the initial extracted beam, aberration characteristics appear in simulations that are also present in experimental phase space current density measurements. Further, measurements of phase space tilt indicate that simulations must have little or no space charge neutralization along the transport system to best agree with experiment. In addition, recent measurements of triangular beam structure immediately after the source are presented. This beam structure is related to the source magnetic confinement fields and will need to be taken into account as the initial beam approximations are lifted.

  10. A system identification approach for developing model predictive controllers of antibody quality attributes in cell culture processes.

    Science.gov (United States)

    Downey, Brandon; Schmitt, John; Beller, Justin; Russell, Brian; Quach, Anthony; Hermann, Elizabeth; Lyon, David; Breit, Jeffrey

    2017-11-01

    As the biopharmaceutical industry evolves to include more diverse protein formats and processes, more robust control of Critical Quality Attributes (CQAs) is needed to maintain processing flexibility without compromising quality. Active control of CQAs has been demonstrated using model predictive control techniques, which allow development of processes which are robust against disturbances associated with raw material variability and other potentially flexible operating conditions. Wide adoption of model predictive control in biopharmaceutical cell culture processes has been hampered, however, in part due to the large amount of data and expertise required to make a predictive model of controlled CQAs, a requirement for model predictive control. Here we developed a highly automated, perfusion apparatus to systematically and efficiently generate predictive models using application of system identification approaches. We successfully created a predictive model of %galactosylation using data obtained by manipulating galactose concentration in the perfusion apparatus in serialized step change experiments. We then demonstrated the use of the model in a model predictive controller in a simulated control scenario to successfully achieve a %galactosylation set point in a simulated fed-batch culture. The automated model identification approach demonstrated here can potentially be generalized to many CQAs, and could be a more efficient, faster, and highly automated alternative to batch experiments for developing predictive models in cell culture processes, and allow the wider adoption of model predictive control in biopharmaceutical processes. © 2017 The Authors Biotechnology Progress published by Wiley Periodicals, Inc. on behalf of American Institute of Chemical Engineers Biotechnol. Prog., 33:1647-1661, 2017. © 2017 The Authors Biotechnology Progress published by Wiley Periodicals, Inc. on behalf of American Institute of Chemical Engineers.

  11. A Multiple Sensor Machine Vision System for Automatic Hardwood Feature Detection

    Science.gov (United States)

    D. Earl Kline; Richard W. Conners; Daniel L. Schmoldt; Philip A. Araman; Robert L. Brisbin

    1993-01-01

    A multiple sensor machine vision prototype is being developed to scan full size hardwood lumber at industrial speeds for automatically detecting features such as knots holes, wane, stain, splits, checks, and color. The prototype integrates a multiple sensor imaging system, a materials handling system, a computer system, and application software. The prototype provides...

  12. The Comparison of Layout Arrangements for the Material Flow Ordering Planning in Production Systems through Simulation Analysis

    Directory of Open Access Journals (Sweden)

    Mehmet AKSARAYLI

    2009-02-01

    Full Text Available Enterprises have to make suitable location planning to decrease their product cost and to increase their productivity in our time. The aim of this study is to compare the basic layout types used in arranging inside facility layout with each other by using simulation. Especially in this study, material handling times between machines and ratio of these times in total times were interested. First of all a new production system is designed to the basic layout types used in arranging inside facility layout. And then in the designed production system, machines are arranged for each machine layout types. Machine layout types are transferred to PROMODEL simulation software. Then with the results of analysis, material handling times of different machine layout types and ratio of material handling times in total production time were compared and the results obtained from this analysis were given after commented on this study.

  13. Nuclear Technology Series. Course 25: Radioactive Material Handling Techniques.

    Science.gov (United States)

    Center for Occupational Research and Development, Inc., Waco, TX.

    This technical specialty course is one of thirty-five courses designed for use by two-year postsecondary institutions in five nuclear technician curriculum areas: (1) radiation protection technician, (2) nuclear instrumentation and control technician, (3) nuclear materials processing technician, (4) nuclear quality-assurance/quality-control…

  14. Proceedings - Workshop on Materials Handling for Tunnel Construction

    Science.gov (United States)

    1977-08-01

    With the anticipated increases in tunnel construction in the next decade, greater demands will be made on transportation sytems to remove tunnel muck at rates consistent with tunnel excavation rates. This workshop discussed and noted that conventiona...

  15. 29 CFR 1926.251 - Rigging equipment for material handling.

    Science.gov (United States)

    2010-07-01

    ... working loads and shall be proof-tested prior to use to 125 percent of their rated load. (5) Scope. This... their body diameter. (14) Safe operating temperatures. Fiber core wire rope slings of all grades shall... unless proof tested by the manufacturer or equivalent entity at twice their rated capacity prior to...

  16. Integrating CAD/CAM in Automation and Materials Handling

    Science.gov (United States)

    Deal, Walter F.; Jones, Catherine E.

    2012-01-01

    Humans by their very nature are users of tools, materials, and processes as a part of their survival and existence. As humans have progressed over time, their civilizations and societies have changed beyond imagination and have moved from hunters and gatherers of food and materials for survival to sophisticated societies with complex social and…

  17. Remote material handling in the Plutonium Immobilization Project. Revision 1

    International Nuclear Information System (INIS)

    Brault, J.R.

    2000-01-01

    With the downsizing of the US and Russian nuclear stockpiles, large quantities of weapons-usable plutonium in the US are being declared excess and will be disposed of by the Department of Energy Fissile Materials Disposition Program. To implement this program, DOE has selected the Savannah River Site (SRS) for the construction and operation of three new facilities: pit disassembly and conversion; mixed oxide fuel fabrication; and plutonium immobilization. The Plutonium Immobilization Project (PIP) will immobilize a portion of the excess plutonium in a hybrid ceramic and glass form containing high level waste for eventual disposal in a geologic repository. The PIP is divided into three distinct operating areas: Plutonium Conversion, First Stage Immobilization, and Second Stage Immobilization. Processing technology for the PIP is being developed jointly by the Lawrence Livermore National Laboratory and Westinghouse Savannah River Company. This paper will discuss development of the automated unpacking and sorting operations in the conversion area, and the automated puck and tray handling operations in the first stage immobilization area. Due to the high radiation levels and toxicity of the materials to be disposed of, the PIP will utilize automated equipment in a contained (glovebox) facility. Most operations involving plutonium-bearing materials will be performed remotely, separating personnel from the radiation source. Source term materials will be removed from the operations during maintenance. Maintenance will then be performed hands on within the containment using glove ports

  18. Utilizing Technology in Manual Material Handling and Safe Lifting.

    Science.gov (United States)

    Snyder, Mick

    2016-02-01

    There is great potential to decrease injuries with the use of these new technologies, especially musculoskeletal disorders and repetitive task-related injuries. Initial costs can be considerable for some of these units, but they are much cheaper than a back surgery. As with all technology, the first designs cost a small fortune, but as we are seeing even now, the pricing is decreasing and the quality is increasing for these devices. In 30 years, we might all have flying cars like "Back to the Future II" predicted we would in 2015 or be able to figure out a tricorder like on "Star Trek"! For more information on exoskeletons, exoskeletonreport.com is a great resource.

  19. Coordinated Mobile Manipulation for Robotics Material Handling, Phase I

    Data.gov (United States)

    National Aeronautics and Space Administration — Robots will be precursors to human exploration of the lunar surface. They will be expected to prepare the lunar surface for human habitation as well as conduct...

  20. Processing device for discharged water from radioactive material handling facility

    International Nuclear Information System (INIS)

    Kono, Takao; Kono, Hiroyuki; Yasui, Katsuaki; Kataiki, Koichi.

    1995-01-01

    The device of the present invention comprises a mechanical floating material-removing means for removing floating materials in discharged water, an ultrafiltration device for separating processed water discharged from the removing means by membranes, a reverse osmotic filtration device for separating the permeated water and a condensing means for evaporating condensed water. Since processed water after mechanically removing floating materials is supplied to the ultrafiltration device, the load applied on the filtering membrane is reduced, to simplify the operation control as a total. In addition, since the amount of resultant condensed water is reduced, and the devolumed condensed water is condensed and dried, the condensing device is made compact and the amount of resultant wastes is reduced. (T.M.)

  1. Mobile materials handling platform interface architecture for mass production environments

    CSIR Research Space (South Africa)

    Walker, A

    2008-01-01

    Full Text Available the processing environment. Due to the operational structure of mass customisation, passive methods alone cannot facilitate customer influenced production dynamics. This is due to the fact that every product is different from the last. Active methods...

  2. Coordinated Mobile Manipulation for Robotics Material Handling, Phase II

    Data.gov (United States)

    National Aeronautics and Space Administration — Robots will play an important role in NASA's exploration activities over the next several decades. They will land on the Lunar surface ahead of humans and help...

  3. Design guides for radioactive-material-handling facilities and equipment

    International Nuclear Information System (INIS)

    Doman, D.R.; Barker, R.E.

    1980-01-01

    Fourteen key areas relating to facilities and equipment for handling radioactive materials involved in examination, reprocessing, fusion fuel handling and remote maintenance have been defined and writing groups established to prepare design guides for each areas. The guides will give guidance applicable to design, construction, operation, maintenance and safety, together with examples and checklists. Each guide will be reviewed by an independent review group. The guides are expected to be compiled and published as a single document

  4. Hydraulic Servo Systems : Dynamic Properties and Control

    OpenAIRE

    Rydberg, Karl-Erik

    2016-01-01

    When closed-loop hydraulic control systems first began to appear in industry, the applications were generally those in which very high performance was required. While hydraulic servo systems are still heavily used in high-performance applications such as the machine-tool industry, they are beginning to gain wide acceptance in a variety of industries. Examples are material handling, mobile equipment, plastics, steel plants, mining, oil exploration and automotive testing Closed loop servo drive...

  5. Development of RFID Library Management Information System

    OpenAIRE

    Keshinro K. K1 ., Balogun W. A; Oyetola J. B3 ., Omogoye S. O4

    2016-01-01

    : RFID-based systems move beyond security to become tracking systems that combine security with more efficient tracking of materials throughout the library, including easier and faster charge and discharge, inventorying, and materials handling. This technology helps librarians reduce valuable staff time spent scanning barcodes while charging and discharging items. RFID is a combination of radio -frequency-based technology and microchip technologyThe methodology used was serial com...

  6. How do low/high height and weight variation affect upper limb movements during manual material handling of industrial boxes? Como a variação de altura e massa da carga afetam os movimentos do membro superior durante o manuseio de caixas industriais?

    Directory of Open Access Journals (Sweden)

    Ana B. Oliveira

    2011-12-01

    Full Text Available OBJECTIVES: To evaluate the effect of surface height and load weight on upper limb movements and electromyographic (EMG recordings during manual handling performed by both experienced and inexperienced lifter subjects. METHODS: Sixteen experienced and sixteen inexperienced lifters handled a box (both 7 and 15 kg from an intermediate height (waist level to either a high or low surface. Electromyography and video images were recorded during the tasks. The 10th, 50th and 90th percentiles were calculated for the deltoid and biceps muscles, shoulder flexion, shoulder abduction, and elbow flexion movements. Groups, right/left sides, weights and heights were compared. There were no differences between either groups or sides. RESULTS: Weight and height variations affected EMG and posture, although weight had more impact on EMG. Shoulder abduction and flexion movements higher than 60º occurred, particularly for the higher surface. Shoulder flexion was also higher when the box was moved to the low height. This study provides new evidence as shoulder postures during boxes handling on low surfaces had not previously been evaluated. CONCLUSIONS: The high demand of upper limb in manual material handling tasks is clear, particularly for the shoulder. This knowledge can be used by physical therapists to plan better rehabilitation programs for manual material handling-related disorders, particularly focusing on return to work.OBJETIVOS: Avaliar o efeito da altura de superfície e massa da carga nos movimentos e na atividade eletromiográfica (EMG dos membros superiores durante o manuseio de carga realizado por sujeitos experientes e inexperientes. MÉTODOS: Dezesseis sujeitos experientes e 16 inexperientes manusearam uma caixa (7 e 15 kg de uma superfície com altura intermediária para uma superfície alta e/ou baixa. Durante as tarefas, foram registradas imagens de vídeo e EMG. Os dados foram processados para obtenção dos percentis 10, 50 e 90 referentes

  7. Expression and regulation of CYP17A1 and 3β-hydroxysteroid dehydrogenase in cells of the nervous system

    DEFF Research Database (Denmark)

    Emanuelsson, I; Almokhtar, M; Wikvall, K

    2018-01-01

    Steroids are reported to have diverse functions in the nervous system. Enzymatic production of steroid hormones has been reported in different cell types, including astrocytes and neurons. However, the information on some of the steroidogenic enzymes involved is insufficient in many respects....... Contradictory results have been reported concerning the relative importance of different cell types in the nervous system for expression of CYP17A1 and 3β-hydroxysteroid dehydrogenase (3β-HSD). 3β-HSD is important in all basic steroidogenic pathways and CYP17A1 is required to form sex hormones. In the current....... These data suggest that vitamin D-mediated regulation of CYP17A1 and 3β-HSD, particularly on the transcriptional level, may play a role in the nervous system....

  8. An improved out-cell to in-cell rapid transfer system at the HFEF/South [Hot Fuel Examination Facility-South

    International Nuclear Information System (INIS)

    Bacca, J.P.; Sherman, E.K.

    1990-01-01

    Argonne National Laboratory's Fuel Cycle Facility (FCF) 1-4 (formerly named Hot Fuel Examination Facility-South (HFEF/South)) is currently being refurbished and upgraded in preparation for demonstrating remote, fast reactor metal-fuel reprocessing and refabrication, as part of the Integral Fast Reactor (IFR) Program. Among the FCF hot-cell system upgrades being provided is a newly fabricated, direct, out-of-cell to in-cell, small-item transfer system for the FCF argon cell. This system will enable the rapid transfer of selected small items from the hot cell exterior into the argon cell (argon-gas atmosphere) of the facility, without necessitating the use of formerly employed, very time-consuming, and quite laborious procedures. The new system will be especially valuable for the rapid insertion of IFR fuel processing makeup materials and small tools into the argon cell, and for use in argon cell and overall FCF radioactive contamination-control activities. 4 refs., 8 figs

  9. Applications of the systems theory to the designing of the sand preparation sub-system in foundry plants

    Directory of Open Access Journals (Sweden)

    R. Wrona

    2009-07-01

    Full Text Available This study provides the basic principles for designing the functional structure of manufacturing systems and their components. The analysis of functional values is applied to create the technological and manufacturing model underlying the design of the foundry equipment with machine units and materials handling systems. Quoted examples illustrate the approved procedure to be applied to control the sand preparation process in a foundry.

  10. Automatic generation of computer models through the integration of production systems design software tools

    OpenAIRE

    Dias, Luís M. S.; Vik, Pavel; Pereira, Guilherme; Oliveira, José A.

    2010-01-01

    The design of production and logistic systems is a process of managing both technical and organizational variants in order to identify the best solution for a given system. This is a very well-known industrial engineering issue, where the objectives for designing such a system have been changing over the last decades. Former approaches were concerned about material handling costs only but more recent works include re-layout and product mix costs, together with a great concern on processes – h...

  11. Remote Handling Equipment for a High-Level Waste Package Closure System

    International Nuclear Information System (INIS)

    Kevin M. Croft; Scott M. Allen; Mark W. Borland

    2006-01-01

    High-level waste will be placed in sealed waste packages inside a shielded closure cell. The Idaho National Laboratory (INL) has designed a system for closing the waste packages including all cell interior equipment and support systems. This paper discusses the material handling aspects of the equipment used and operations that will take place as part of the waste package closure operations. Prior to construction, the cell and support system will be assembled in a full-scale mockup at INL

  12. Remote Handling Equipment for a High-Level Waste Waste Package Closure System

    Energy Technology Data Exchange (ETDEWEB)

    Kevin M. Croft; Scott M. Allen; Mark W. Borland

    2006-04-01

    High-level waste will be placed in sealed waste packages inside a shielded closure cell. The Idaho National Laboratory (INL) has designed a system for closing the waste packages including all cell interior equipment and support systems. This paper discusses the material handling aspects of the equipment used and operations that will take place as part of the waste package closure operations. Prior to construction, the cell and support system will be assembled in a full-scale mockup at INL.

  13. Transfer tunnel transporter system for the Fuels and Materials Examination Facility

    International Nuclear Information System (INIS)

    Petty, J.A.; Miller, S.C.; Richards, J.T.

    1981-01-01

    The detail design is complete and fabrication is approximately 75% complete on the Transfer Tunnel Transporter System. This system provides material handling capability for large, bulky equipment between two hot cells in a new Breeder Reactor Program support facility, the Fuels and Materials Examination Facility. One hot cell has an air atmosphere, the other a high purity inert gas atmosphere which must be maintained during transfer operations. System design features, operational capabilities and remote recovery provisions are described

  14. Material control system simulator program reference manual

    International Nuclear Information System (INIS)

    Hollstien, R.B.

    1978-01-01

    A description is presented of a Material Control System Simulator (MCSS) program for determination of material accounting uncertainty and system response to particular adversary action sequences that constitute plausible material diversion attempts. The program is intended for use in situations where randomness, uncertainty, or interaction of adversary actions and material control system components make it difficult to assess safeguards effectiveness against particular material diversion attempts. Although MCSS may be used independently in the design or analysis of material handling and processing systems, it has been tailored toward the determination of material accountability and the response of material control systems to adversary action sequences

  15. Material control system simulator program reference manual

    Energy Technology Data Exchange (ETDEWEB)

    Hollstien, R.B.

    1978-01-24

    A description is presented of a Material Control System Simulator (MCSS) program for determination of material accounting uncertainty and system response to particular adversary action sequences that constitute plausible material diversion attempts. The program is intended for use in situations where randomness, uncertainty, or interaction of adversary actions and material control system components make it difficult to assess safeguards effectiveness against particular material diversion attempts. Although MCSS may be used independently in the design or analysis of material handling and processing systems, it has been tailored toward the determination of material accountability and the response of material control systems to adversary action sequences.

  16. Automatic guided vehicles fleet size optimization for flexible manufacturing system by grey wolf optimization algorithm

    Directory of Open Access Journals (Sweden)

    V. K. Chawla

    2018-02-01

    Full Text Available Automatic guided vehicle system (AGVs plays a vital role in material handling operations for a flexible manufacturing system (FMS.Optimum AGVs fleet size selection is one of the most sig-nificant decisions in effective design and control of automated material handling system. The fleet size estimation and optimization of AGVs requires an in-depth understanding of the various factors that AGVs in the FMS relies on. In this paper, an investigation for fleet size optimization of AGVs in different layouts of FMS by application of the analytical method and grey wolf optimization al-gorithm (GWO is carried out. Layout design is one of the significant factors for optimization of AGV’s fleet size in any FMS. Results yield from analytical and grey wolf optimization algorithm are compared and validated for the different sizes of FMS layouts by computational experiments.

  17. A Clonal Selection Algorithm for Minimizing Distance Travel and Back Tracking of Automatic Guided Vehicles in Flexible Manufacturing System

    Science.gov (United States)

    Chawla, Viveak Kumar; Chanda, Arindam Kumar; Angra, Surjit

    2018-03-01

    The flexible manufacturing system (FMS) constitute of several programmable production work centers, material handling systems (MHSs), assembly stations and automatic storage and retrieval systems. In FMS, the automatic guided vehicles (AGVs) play a vital role in material handling operations and enhance the performance of the FMS in its overall operations. To achieve low makespan and high throughput yield in the FMS operations, it is highly imperative to integrate the production work centers schedules with the AGVs schedules. The Production schedule for work centers is generated by application of the Giffler and Thompson algorithm under four kind of priority hybrid dispatching rules. Then the clonal selection algorithm (CSA) is applied for the simultaneous scheduling to reduce backtracking as well as distance travel of AGVs within the FMS facility. The proposed procedure is computationally tested on the benchmark FMS configuration from the literature and findings from the investigations clearly indicates that the CSA yields best results in comparison of other applied methods from the literature.

  18. 14 CFR 417.409 - System hazard controls.

    Science.gov (United States)

    2010-01-01

    ... satisfy the requirements of § 417.123. (b) Structures and material handling equipment. A launch operator must ensure safety factors applied in the design of a structure or material handling equipment account... operator must: (1) Inspect structures and material handling equipment to verify workmanship, proper...

  19. Cell and Tissue Organization in the Circulatory and Ventilatory Systems Volume 1 Signaling in Cell Organization, Fate, and Activity, Part A Cell Structure and Environment

    CERN Document Server

    Thiriet, Marc

    2011-01-01

    The volumes in this authoritative series present a multidisciplinary approach to modeling and simulation of flows in the cardiovascular and ventilatory systems, especially multiscale modeling and coupled simulations. The cardiovascular and respiratory systems are tightly coupled, as their primary function is to supply oxygen to and remove carbon dioxide from the body's cells. Because physiological conduits have deformable and reactive walls, macroscopic flow behavior and prediction must be coupled to nano- and microscopic events in a corrector scheme of regulated mechanisms. Therefore, investigation of flows of blood and air in physiological conduits requires an understanding of the biology, chemistry, and physics of these systems together with the mathematical tools to describe their functioning.  The present volume is devoted to cellular events that allow adaptation to environmental conditions, particularly mechanotransduction. It begins with cell organization and a survey of cell types in the vasculatur...

  20. DNA repair proteins in cells of the human immune system; Le proteine della riparazione del DNA in cellule del sistema immunitario umano

    Energy Technology Data Exchange (ETDEWEB)

    Frasca, D.; Barattini, P.; Guidi, F.; Scarpaci, S. [ENEA, Sez. Tossicologia e Scienze Biomediche, Rome (Italy); Doria, G. [Rome Univ. Tor Vergata, Rome (Italy). Cattedra di Immunologia

    2001-02-01

    Human longevity depends on the efficiency of DNA repair mechanisms. In irradiated cells of the human immune system, the principal repair mechanism involves the DNA-Pk protein complex. [Italian] La durata della vita dipende dalla efficienza di meccanismi di riparazione del DNA. Nelle cellule del sistema immunitario umano danneggiate il principale meccanismo di riparazione coinvolge il complesso proteico DNA-PK.

  1. Robotics in a controlled, ecological life support system

    Science.gov (United States)

    Miles, Gaines E.; Krom, Kimberly J.

    1993-01-01

    Controlled, Ecological Life Support Systems (CELSS) that utilize plants to provide food, water and oxygen could consume considerable amounts of labor unless crop production, recovery and processing are automated. Robotic manipulators equipped with special end-effectors and programmed to perform the sensing and materials handling tasks would minimize the amount of astronaut labor required. The Human Rated Test Facility (HRTF) planned for Johnson Space Center could discover and demonstrate techniques of crop production which can be reliably integrated with machinery to minimize labor requirements. Before the physical components (shelves, lighting fixtures, etc.) can be selected, a systems analysis must be performed to determine which alternative processes should be followed and how the materials handling tasks should be automated. Given that the current procedures used to grow crops in a CELSS may not be the best methods to automate, then what are the alternatives? How may plants be grown, harvested, processed for food, and the inedible components recycled? What commercial technologies current exist? What research efforts are underway to develop new technologies which might satisfy the need for automation in a CELSS? The answers to these questions should prove enlightening and provide some of the information necessary to perform the systems analysis. The planting, culturing, gathering, threshing and separation, food processing, and recovery of inedible portions of wheat were studied. The basic biological and materials handling processes of each task are defined and discussed. Current practices at Johnson Space Center and other NASA centers are described and compared to common production practices in the plant production industry. Technologies currently being researched which might be applicable are identified and illustrated. Finally, based on this knowledge, several scenarios are proposed for automating the tasks for wheat.

  2. CAD system applications to the nuclear fuel reprocessing facilities

    International Nuclear Information System (INIS)

    Morita, Eiji; Matsumoto, Tadakuni; Shikakura, Sakae; Furuya, Kousei; Sakurai, Shin-ichi.

    1994-01-01

    Effective supporting techniques of design, operation, and maintenance of the reprocessing facility have been developed using the Intergraph CAD system. Two and three dimensional views of the process cells were utilized to rationalize the equipment layout and material handling flows, and to check the piping interference. Interferences of the remote maintenance equipment with the process equipments were also evaluated by the pictures on the CAD display. The newest virtual reality technology will help our future development of the more natural simulation for the remote maintenance operator training. (author)

  3. Seminars in Cell and Developmental Biology Model Systems for the Study of Heart Development and Disease Cardiac Neural Crest and Conotruncal Malformations

    Science.gov (United States)

    Hutson, Mary R.; Kirby, Margaret L.

    2007-01-01

    Neural crest cells are multipotential cells that delaminate from the dorsal neural tube and migrate widely throughout the body. A subregion of the cranial neural crest originating between the otocyst and somite 3 has been called “cardiac neural crest” because of the importance of these cells in heart development. Much of what we know about the contribution and function of the cardiac neural crest in cardiovascular development has been learned in the chick embryo using quail-chick chimeras to study neural crest migration and derivatives and using ablation of premigratory neural crest cells to study their function. These studies show that cardiac crest cells are absolutely required to form the aorticopulmonary septum dividing the cardiac arterial pole into systemic and pulmonary circulations. They support the normal development and patterning of derivatives of the caudal pharyngeal arches and pouches, including the great arteries and the thymus, thyroid and parathyroids. Recently, cardiac neural crest cells have been shown to modulate signaling in the pharynx during the lengthening of the outflow tract by the secondary heart field. Most of the genes associated with cardiac neural crest function have been identified using mouse models. These studies show that the neural crest cells may not be the direct cause of abnormal cardiovascular development but they are a major component in the complex tissue interactions in the caudal pharynx and outflow tract. Since cardiac neural crest cells span from the caudal pharynx into the outflow tract, they are especially susceptible to any perturbation in or by other cells in these regions. Thus, understanding congenital cardiac outflow malformations in human sequences of malformations as represented by the DiGeorge syndrome will necessarily require understanding development of the cardiac neural crest. PMID:17224285

  4. [Biomechanical risk assessment of manual material handling in vegetables and fruit departments of supermarkets].

    Science.gov (United States)

    Draicchio, F; Silvetti, A; Badellino, E; Vinci, F

    2007-01-01

    There is little in the literature about the risks of manual handling of material in supermarkets and what there is refers solely to storehouse work. This contrasts with the substantial number of studies of the risk of repeated arm movements among supermarket cash-desk staff. The scarcity of information is partly due to the difficulties of applying widely employed, standardized evaluation methods in this sector. One of the conditions limiting the application of the NIOSH protocol in this retail sector is that lifting tasks are so often closely tied to transport. The biomechanical analysis method we used brought to light considerable risks in many of the steps investigated: unpacking the pallet, unloading the crates from the pallet to the ground, lifting them from the floor onto display stands, and filling the boxes on the stands with goods before the shop opens. Images acquired on site were analyzed in the laboratory. We selected the most indicative images, which were then studied as regards posture and biomechanics using Apalys 3.0 software (ILMCAD GmbH, Ilmenau, Germany). Biomechemical analysis was done on the following movements: unloading crates from the pallet, positioning them on fruit and vegetable department display stands, and filling the boxes on the stands. We obtained a prediction of 2720 to 5472 N for the load at the lumbosacral junction (L5-S1). Simulation of the NIOSH index gave a value of 2.69 in the only case where the Waters protocol could be applied.

  5. Application work risk of manual material handling operators using different lifting methods

    Science.gov (United States)

    Anizar; Matondang, AR; Sibarani, JA

    2018-02-01

    This study observed the activity of lifting and moving crates containing bottles at a pallet station in a carbonated drink factory. The activity of moving crates, each weighing 15 kg, is performed by four operators using different methods. An operator manually moves 250 crates daily and often takes rest during working. This study aims to find the most efficient method, that is using the least energy, to move crates. The workload is assessed using the method of Cardiovascular Strain Load (CVL) and energy expenditure. Operators’ arterial pulse is measured for 1 minute right before they start working and after they finish working; this is done to obtain work arterial pulse and rest arterial pulse. The way operators lift crates is analyzed using biomechanics. It is found that fatigue is experienced by all operators, with the exception of the 3rd operator who has a %CVL number below 30%. He positions the pallet to be parallel to the conveyor, and then stand in between during the lifting process. He only rotates his body to move the crate from the conveyor onto the pallet, requiring only little energy. This is one of the reasons why the %CVL number is lower than the other operators.

  6. The Airfloat HL project. [design analysis of airships and lift devices for materials handling

    Science.gov (United States)

    Mowforth, E.

    1975-01-01

    A design study is described for a large low-cost rigid airship intended primarily for the movement of large indivisible loads (cargo) between industrial sites. A survey of the ship and its overall performance is followed by accounts of the operational procedures for the above function and for an alternative application to unit module transfer between fixed terminals. A final section indicates the estimated costs of construction and operation. Safety factors are also considered. Lifting devices such as winches, hoists are shown and described, and airship configurations are also shown.

  7. Picking Routing Problem with K homogenous material handling equipment for a refrigerated warehouse

    Directory of Open Access Journals (Sweden)

    Rodrigo Andrés Gómez-Montoya

    2016-01-01

    Full Text Available Este artículo tiene como objetivo formular un problema de ruteo en la preparación de pedidos con K equipos homogéneos y ventanas de tiempo (PRPHE. Un PSO (Particle Swarm Optimization discreto y un algoritmo Genético (GA, Genetic Algorithm son desarrollados y validados para solucionar PRPHE. El PSO discreto es un enfoque novedoso para resolver el problema de ruteo en la preparación de pedidos en frio, el cual no había sido detectado en la literatura científica por lo cual es considerado una contribución en el estado del arte. La principal diferencia entre el PSO clásico y el discreto desarrollado es la estructura y la formulación algebraica de las posiciones y velocidades de las partículas las cuales son discretas en vez de ser continuas. Un diseño factorial completo con cuatro factores denominados metaheurísticos para el ruteo en la preparación de pedidos (PRM, picking routing metaheuristics, depot, tamaño de listas de preparación (Picking List Size y un grupo de K equipos de manejo de materiales homogéneos (MHE fue desarrollado. Basado en los resultados del análisis experimental fue identificado que el metaheurístico GA generó mejores soluciones que PSO discreto para el PRPHE. Por lo tanto, estos resultados estadísticos demostraron que el metaheurístico GA produjeron ahorros de tiempo entre 22,89 and 86,75 segundos por conjunto de rutas de preparación de pedidos en frio, así como un incremento de la eficiencia de las operaciones entre 1,98 y 2,81%, respecto a el PSO discreto. Finalmente, debe resaltarse que este artículo es uno de los primeros en abordar el ruteo en la preparación de pedidos en almacenes refrigerados de ahí su contribución al conocimiento.

  8. Evaluation of perceived and self-reported manual forces exerted in occupational materials handling.

    Science.gov (United States)

    Wiktorin, C; Selin, K; Ekenvall, L; Kilbom, A; Alfredsson, L

    1996-08-01

    The main objective of the study was to evaluate the ability of workers to reproduce simulated manual work forces correctly and to quantify these forces in Newtons (N) by means of self-reports. Fourteen male and 14 female workers participated in the study. Three experiments were carried out. In the first experiment, the ability to reproduce the magnitudes of simulated manual forces occurring in daily work and to estimate these forces in Newtons was tested. A specially designed force-measuring device was used for this purpose. In the second experiment, the subjects estimated the weights of five boxes ranging from 1 to 30 kg. In the third experiment, the subjects were asked to produce five predetermined push and pull forces ranging in magnitude from 10 to 300 N on to the handle of the force-measuring device. The ability to reproduce the magnitudes of manual forces when simulating four familiar work tasks was good (the intraclass correlation coefficients ranged from 0.75 to 0.95). The ability to quantify these forces in Newtons was not as good (the product moment correlation coefficients ranged from 0.21 to 0.69). When the subjects estimated the weights of boxes they underestimated the weights. When they produced predetermined push and pull forces they exerted higher forces than expected when low force levels were requested and lower forces when high force levels were requested. However, the forces were correctly ranked. In summary, simulation of the manual push/pull forces used in familiar work tasks seemed to offer sufficient reproducibility to be worth testing for validity. Self-reports, used without previous training or without known 'reference forces', seemed to be very rough when the aim was to estimate in kg or Newtons. However, the fact that individuals could rank the forces correctly opens a potential for refinement of self-reports as a method for quantifying manual forces in objective terms, e.g. kg or Newtons.

  9. A study on the corrosion test of equipment material handling hot molten salt

    International Nuclear Information System (INIS)

    Ro, Seung Gy; Jeong, M.S.; Hong, S.S.; Cho, S.H.; Shin, Y.J.; Park, H.S.; Zhang, J.S.

    1999-02-01

    On this technical report, corrosion behavior of austenitic stainless steels of SUS 316L and SUS 304L in molten salt of LiCl-Li 2 O has been investigated in the temperature range of 650 - 850 dg C. Corrosion products of SUS 316L in molten salt consisted of two layers, an outer layer of LiCrO 2 and inner layer of Cr 2 O 3 .The corrosion layer was uniform in molten salt of LiCl, but the intergranular corrosion occurred in addition to the uniform corrosion in mixed molten salt of LiCl-Li 2 O. The corrosion rate increased slowly with the increase of temperature up to 750 dg C, but above 750 dg C rapid increase in corrosion rate observed. SUS 316L stainless steel showed slower corrosion rate and higher activation energy for corrosion than SUS 304L, exhibiting higher corrosion resistance in the molten salt. In heat-resistant alloy, dense protective oxide scale of LiCrO 2 was formed in molten salt of LiCl. Whereas in mixed molten salt of LiCl-Li 2 O, porous non-protective scale of Li(Cr, Ni, Fe)O 2 was formed. (Author). 44 refs., 4 tabs., 16 figs

  10. Performance of Orius insidiosus after storage, exposure to dispersal material, handling and shipment processes

    NARCIS (Netherlands)

    Bueno, V.H.P.; Carvalho, L.M.; Lenteren, van J.C.

    2014-01-01

    Storage, handling and shipment procedures are important factors influencing the quality of biological control agents. This study aimed to evaluate biological parameters and performance of Orius insidiosus (Say) after different storage periods at low temperatures, after exposure to different

  11. Review of the Strength and Capacity Data for Manual Material Handling Activities.

    Science.gov (United States)

    1979-11-01

    Then strength of the abdominal and psoas muscles were determined for the second group. RESULTS: 1. There was a significant difference in the strength ... strength of the abdominal and psoas muscles was determined. The results led to the following con- clusions: Chronic low back pain not associated with...AD-AG92 313 TEXAS TECH UNIV LUBBOCK INST FOR BIOTECHNOLOGY FIG 6/14 REVIEW OF THE STRENGTH AND CAPACITY DATA FOR MANUAL MATERIAL HA--ETC(U) NOV 79 m

  12. Pneubotics - Membrane-Based Robotics for Remote Material Handling, Phase II

    Data.gov (United States)

    National Aeronautics and Space Administration — We have invented a new class of robotics, called `Pneubotics', that rival current manipulators in payload and reach at 1/10th the weight. Our technology leverages...

  13. Standard handbook of chains chains for power transmission and material handling

    CERN Document Server

    2005-01-01

    A BRIEF HISTORY OF THE DEVELOPMENT OF CHAINEarly DevelopmentsCog ChainCast Detachable ChainCast Pintle ChainPrecision Roller ChainEngineering Steel ChainSilent ChainFlat-Top ChainTerminologyA CHAIN OVERVIEW: USES AND ADVANTAGESGeneralTypes of ChainScope of Chains CoveredStyles and Forms of ChainsStraight and Offset Link ChainsChains With and Without RollersUses of ChainStandard Chains and Their UsesThe Advantages of Chains in ApplicationsAdvantages of Roller Chains in DrivesAdvantages of Silent Chain Drives

  14. Pneubotics - Membrane-Based Robotics for Remote Material Handling, Phase I

    Data.gov (United States)

    National Aeronautics and Space Administration — We have invented a new class of robotics, called `Pneubotics', that rival current manipulators in payload and reach at 1/10th the weight. Our technology leverages...

  15. Analysis of Material Handling Equipment for Maritime Repositioning Ships (MPS) Instream Offload

    OpenAIRE

    Sumner, John D.; Kang, Keebom

    1992-01-01

    Proceedings of the 1992 Winter Simulation Conference, ed. J. J. Swain, D. Goldsman, R. C. Crain and J.R. Wilson The Maritime Prepositioning Force (MPF) program enables the United States Marine Corps to globally project rapid and sustainable combat power. The instream method of offloading equipment and supplies from the Maritime Prepositioning Ships (MPS) provides the flexibility needed to respond to a broader range of contingencies dictated by the increasingly dynamic ...

  16. Analysis of material handling equipment for Maritime Prepositioning Ships (MPS) instream offload

    OpenAIRE

    Sumner, John D.; Kang, Keebom

    1992-01-01

    The Maritime Prepositioning Force (MPF) program enables the United States Marine Corps to globally project rapid and sustainable combat power. The instream method of offloading equipment and supplies from the Maritime Prepositioning Ships (MPS) provides the flexibility needed to respond to a broader range of contingencies dictated by the increasingly dynamic geostrategic environment. In this paper, we develop a simulation model of an MPS instream offloading operation to provide Marine Corps c...

  17. Criticality in cell differentiation

    Indian Academy of Sciences (India)

    The differentiation dynamics have both deterministic andstochastic components. Several theoretical studies suggest that cell differentiation is a bifurcation phenomenon, well-knownin dynamical systems theory. The bifurcation point has the character of a critical point with the system dynamics exhibitingspecific features in its ...

  18. PEMFC MEA and System Design Considerations

    Energy Technology Data Exchange (ETDEWEB)

    Knights, Shanna [Ballard Power Systems, Inc.; Bashyam, Rajesh [Ballard Power Systems, Inc.; He, Ping [Ballard Power Systems, Inc.; Lauritzen, Michael [Ballard Power Systems, Inc.; Startek, Cara [Ballard Power Systems, Inc.; Colbow, Vesna [Ballard Power Systems, Inc.; Cheng, Tommy [Ballard Power Systems, Inc.; Kolodziej, Joanna [Ballard Power Systems, Inc.; Wessel, Silvia [Ballard Power Systems, Inc.

    2011-07-01

    Proton exchange membrane fuel cells (PEMFCs) are being developed and sold commercially for multiple near term markets. Ballard Power Systems is focused on the near term markets of backup power, distributed generation, materials handling, and buses. Significant advances have been made in cost and durability of fuel cell products. Improved tolerance to a wide range of system operation and environmental noises will enable increased viability across a broad range of applications. In order to apply the most effective membrane electrode assembly (MEA) design for each market, the system requirements and associated MEA failures must be well understood. The failure modes associated with the electrodes and membrane degradation are discussed with respect to associated system operation and mitigating approaches. A few key system considerations that influence MEA design include expected fuel quality, balance-of-plant materials, time under idle or open circuit operation, and start-up and shut-down conditions.

  19. Towards systems thinking in cell biology education

    NARCIS (Netherlands)

    Verhoeff, Roald Pieter

    2003-01-01

    Students are taught a large variety of life structures and processes at the cellular level. The concepts used to describe them are mainly drawn from the sub-cellular level, but this knowledge seems to be fragmentary if its integration at the cellular and organismic level remains undone. As a

  20. Medium and high energy electron beam processing system

    Energy Technology Data Exchange (ETDEWEB)

    Kashiwagi, Masayuki [Nissin-High Voltage Co., Ltd., Kyoto (Japan)

    2003-02-01

    Electron Beam Processing System (EPS) is a useful and powerful tool for industrial irradiation process. The specification of EPS is decided by consideration to irradiate what material with how thick and wide, how much dose, how to handle, in what atmosphere. In designing an EPS, it is necessary to consider safety measure such as x-ray shielding, ozone control and interlock system. The initial costs to install typical EPS are estimated for acceleration voltages from 500 kV to 5 MV, including following items; those are electron beam machine, x-ray shielding, auxiliary equipment, material handling, survey for installation, ozone exhaust duct, cooling water system, wiring and piping. These prices are reference only because the price should be changed for each case. The price of x-ray shielding should be changed by construction cost. Auxiliary equipment includes window, cooling blower, ozone exhaust blower and SF6 gas handling equipment. In installation work at site, actual workers of 3 - 4 persons for 2 months are necessary. Material handling system is considered only rolls provided in the shielding room as reference. In addition to the initial installation, operators and workers may be required to wear a personal radiation monitor. An x-ray monitor of suitable design should be installed outside the shield room to monitor x-ray level in the working area. (Y. Tanaka)

  1. Genes involved in cell division in mycoplasmas

    OpenAIRE

    Alarcón, Frank; Vasconcelos, Ana Tereza Ribeiro de; Yim, Lucia; Zaha, Arnaldo

    2007-01-01

    Bacterial cell division has been studied mainly in model systems such as Escherichia coli and Bacillus subtilis, where it is described as a complex process with the participation of a group of proteins which assemble into a multiprotein complex called the septal ring. Mycoplasmas are cell wall-less bacteria presenting a reduced genome. Thus, it was important to compare their genomes to analyze putative genes involved in cell division processes. The division and cell wall (dcw) cluster, which ...

  2. A system of UAV application in indoor environment

    Directory of Open Access Journals (Sweden)

    Yohanes Khosiawan

    2016-01-01

    Full Text Available In recent years, there has been an increased demand in the use of multiple unmanned aerial vehicles (UAVs in indoor environments such as material handling task in a manufacturing environment and plant/environment monitoring task in a greenhouse. However, there is a lack of work reported on this topic. This paper presents a detailed study on several UAV systems and UAV scheduling systems. It is followed by a proposed system of UAV application in indoor environment, which comprises components of UAV system addressed in detail; focused on scheduler as the heart of operations. Consequently, system architecture of UAV scheduling system is presented and the framework of scheduler component is included. Scheduler component works in a phased manner to provide a systematic abstraction and achieve an efficient computation time. This study serves as a reference guide for UAV application in indoor environment.

  3. Dipole magnet shuttle system

    International Nuclear Information System (INIS)

    Zinszer, A.; Pidcoe, S.; Spann, K.

    1992-01-01

    A transport system has been developed to move major magnet subassemblies between tool stations. The need existed to find a more efficient solution than overhead cranes to handle large parts. The argument against overhead cranes includes safety concerns, work disruption, particulate contamination and meeting the assembly rate requirements of ten magnets per day. The shuttle transport system represents a major effort of coordination between the various tool suppliers and General Dynamics to design a universal device capable of bridging the gap from single wound coils to a complete CDM. Effort was directed to systematically minimize material handling and related equipment by interfacing a completed assembly directly into the next work station or tool without losing its orientation or changing pickup points. The shuttle transport system is made up of a common transport device which can automatically go to any preprogrammed address on the factory floor. Each station has unique attachment tooling which can interface with the shuttle and the next assembly station. The shuttle can also circulate attachment tools back to their point of origin. Additional benefits of this system include inherent part protection, flow control, reduced banking or inventory, and potential for automatic control

  4. Analysis of reconfigurable assembly system framing systems in automotive industry

    Directory of Open Access Journals (Sweden)

    Md Zain Mohamad Zamri

    2017-01-01

    Full Text Available Current trend in automotive industry shows increasing demand for multiple models with lean production. Prior to that, automotive manufacturing systems evolved from mass production to flexible automation. Material handling systems and equipment in a single assembly line with multiple models require high investment but with low throughput thus making production cost relatively high. Current assembly process of side structure and undercarriage with downtime occurrence during assembly process affecting production performance (quality, cost and delivery. Manufacturing facilities should allow more flexibility and increase intelligence evolving toward novel reconfigurable assembly systems (RAS. RAS is envisaged capable of increasing factor flexibility and responsiveness by incorporating assembly jig, robot and framing, which could be next generation of world class automotive assembly systems. This project research proposes a new methodology of framework reconfigurable assembly systems principles in automotive framing systems i.e. enhance assembly process between side structure assembly and undercarriage assembly which a new RAS is capable to reconfigure the assembly processes of multiple model on a single assembly line. Simulation software (Witness will be used to simulate and validate current and proposed assembly process. RAS is expected to be a solution for rapid change in structure and for a responsively adjustable production capacity. Quality, cost and delivery are production key parameters that can be achieved by implementing RAS.

  5. New Concepts and Theories For Intelligent Control of Cellular Manufacturing Systems

    DEFF Research Database (Denmark)

    Langer, Gilad

    1996-01-01

    This paper will present some new theories such as biological manufacturing system, the fractal factory theory, holonic manufacturing systems, agile manufacturing, object orientation, multi-agent theory, artificial intelligence, and artificial life in the context of manufacturing systems....... The paper tries to encapsulate the main area of my Ph.D. thesis research which will evolve around the idea of integrating intelligent elements into the control systems of the manufacturing systems. Furthermore it intends to show how the curriculum and discussions of the IPS Ph.D. course will and have...... contributed to my research. The research will concentrate on integration of manufacturing units by use of intelligent control mechanisms, information technology and the material handling as the key integrators....

  6. Basic statistics in cell biology.

    Science.gov (United States)

    Vaux, David L

    2014-01-01

    The physicist Ernest Rutherford said, "If your experiment needs statistics, you ought to have done a better experiment." Although this aphorism remains true for much of today's research in cell biology, a basic understanding of statistics can be useful to cell biologists to help in monitoring the conduct of their experiments, in interpreting the results, in presenting them in publications, and when critically evaluating research by others. However, training in statistics is often focused on the sophisticated needs of clinical researchers, psychologists, and epidemiologists, whose conclusions depend wholly on statistics, rather than the practical needs of cell biologists, whose experiments often provide evidence that is not statistical in nature. This review describes some of the basic statistical principles that may be of use to experimental biologists, but it does not cover the sophisticated statistics needed for papers that contain evidence of no other kind.

  7. Real-time, interactive, visually updated simulator system for telepresence

    Science.gov (United States)

    Schebor, Frederick S.; Turney, Jerry L.; Marzwell, Neville I.

    1991-01-01

    Time delays and limited sensory feedback of remote telerobotic systems tend to disorient teleoperators and dramatically decrease the operator's performance. To remove the effects of time delays, key components were designed and developed of a prototype forward simulation subsystem, the Global-Local Environment Telerobotic Simulator (GLETS) that buffers the operator from the remote task. GLETS totally immerses an operator in a real-time, interactive, simulated, visually updated artificial environment of the remote telerobotic site. Using GLETS, the operator will, in effect, enter into a telerobotic virtual reality and can easily form a gestalt of the virtual 'local site' that matches the operator's normal interactions with the remote site. In addition to use in space based telerobotics, GLETS, due to its extendable architecture, can also be used in other teleoperational environments such as toxic material handling, construction, and undersea exploration.

  8. Development of 2D particle-in-cell code to simulate high current, low ...

    Indian Academy of Sciences (India)

    Home; Journals; Pramana – Journal of Physics; Volume 69; Issue 4. Development of 2D particle-in-cell code to simulate high current, low energy beam in a beam transport system. S C L Srivastava S V L S Rao ... Keywords. Space-charge; particle-in-cell; beam dynamics; Poisson's equation; solenoids; quadrupole magnets.

  9. A history of low back pain affects pelvis and trunk coordination during a sustained manual materials handling task

    Directory of Open Access Journals (Sweden)

    Joseph F. Seay

    2016-03-01

    Conclusion: Differences between groups demonstrate residual consequences of LBP in an occupational scenario, even though the HBP group was pain-free for >6 months prior to data collection. More in-phase coordination in the HBP group may represent a coordination pattern analogous to “guarded gait” which has been observed in other studies, and may lend insight as to why these individuals are at increased risk for re-injury.

  10. The assessment of material handling strategies in dealing with sudden loading: influences of foot placement on trunk biomechanics.

    Science.gov (United States)

    Zhou, Jie; Dai, Boyi; Ning, Xiaopeng

    2013-01-01

    Sudden unexpected loading has been identified as a risk factor of work-related low back pain (LBP). This study investigated the effects of different foot placements and load-releasing locations on trunk biomechanics under an unexpected sudden loading event. Fifteen subjects experienced sudden release of a 6.8-kg external load from symmetric or asymmetric directions while maintaining four different foot placements. The results showed that subjects experienced on average 4.1° less trunk flexion, 6.6 Nm less L5/S1 joint moment and 32.0 N less shear force with staggered stance with the right foot forward (the most preferred placement) compared with wide stance (the least preferred placement). Asymmetric load-releasing positions consistently resulted in smaller impacts on trunk biomechanics than symmetric positions. The findings suggest that staggered stance and asymmetric load-holding position can be used as a protective load-handling posture against LBP caused by sudden loading. In a work environment, unexpected sudden loading may cause low back pain (LBP). In this study, we used empirical data to demonstrate how different foot placements and load-releasing locations can be used to mitigate the impact of sudden loading on the spine and to reduce the risk of LBP.

  11. The assessment of material handling strategies in dealing with sudden loading: the effects of load handling position on trunk biomechanics.

    Science.gov (United States)

    Ning, Xiaopeng; Zhou, Jie; Dai, Boyi; Jaridi, Majid

    2014-11-01

    Back injury caused by sudden loading is a significant risk among workers that perform manual handling tasks. The present study investigated the effects of load handling position on trunk biomechanics (flexion angle, L5/S1 joint moment and compression force) during sudden loading. Eleven subjects were exposed to a 6.8 kg sudden loading while standing upright, facing forward and holding load at three different vertical heights in the sagittal plane or 45° left to the sagittal plane (created by arm rotation). Results showed that the increase of load holding height significantly elevated the peak L5/S1 joint compression force and reduced the magnitude of trunk flexion. Further, experiencing sudden loading from an asymmetric direction resulted in significantly smaller peak L5/S1 joint compression force, trunk flexion angle and L5/S1 joint moment than a symmetric posture. These findings suggest that handling loads in a lower position could work as a protective strategy during sudden loading. Copyright © 2014 Elsevier Ltd and The Ergonomics Society. All rights reserved.

  12. Decree 2210: by means of which technical standards and allowed proceedings for radioactive material handling are issued

    International Nuclear Information System (INIS)

    1992-01-01

    This Decree has the regulation and handling of radioactive material as object, in order to protect the health of people as well as the atmosphere. These regulations are applicable to: all natural or artificial person, public or private, that imports, manufacture, transport, store, trade, transfer or use with industrial, commercial, scientific, medical or any other aim; apparatuses capable to generate ionizing radiations whose quantum energy is superior to 5 k lo electron volts (KeV) or materials that contain radionuclides whose activities surpass the maxim exempt registration; notification and license concession. It includes: definitions; signalizing by means of basic symbol that must be included in all object, material and their mixtures, that emit ionizing radiations; control; production; import and export; trade; use; and transport of activities that involve materials and apparatuses capable to generate ionizing radiations; the category and labeled of bundles; the limits of activity for excepted bundles; the corresponding values for the different radionuclides; the limits of activity for the means of material haulage; the storage; and the handling of radioactive waste [es

  13. Ergonomic interventions for improving working postures associated with manual materials handling (case study: a mineral processing plant

    Directory of Open Access Journals (Sweden)

    Fateme Dehghani

    2016-12-01

    Full Text Available Introduction: A high percentage of musculoskeletal disorders in workplaces occur due to awkward posture and non-ergonomic design of the work stations for lifting and carrying of materials. To avoid these injuries, jobs should be designed in a way that ergonomics risk factors are controlled properly. The aim of this study was to utilize ergonomics interventions to minimize ergonomics risk factors in bag packing unit in a mineral processing plant. Material and Method: This cross sectional study was carried out among 20 workers of bag packing unit. Camera recording of working postures, evaluation of medical records, interview, and REBA technique were used to identify the ergonomic risk factors. Interventions included changing the conveyor belt height and the use of spring pallets (spring table. Data were analyzed using Paired T-Test by SPSS software version 18. Result: Before implementing ergonomics intervention, a total of 75% of evaluated postures by REBA technique obtained score of 8-10 (very high risk level and 25% had score of 11-15 (very high risk level that correspond to the action level 3 and 4, respectively. Following the implementation of ergonomics interventions, a total of 90% of the analyzed postures showed action level 2 (moderate risk level and the remainder 10 percent of evaluated postures showed high risk level. Comparison of REBA technique scores before and after implementing interventions showed a significant difference (P-value < 0.05. Conclusion: Based on the findings of this study, the implementation of ergonomics interventions has remarkably decreased the required action level and it may be able to improve work-related postures.

  14. Is There an Economic Case for Training Intervention in the Manual Material Handling Sector of Developing Countries?

    Science.gov (United States)

    Lahiri, Supriya; Tempesti, Tommaso; Gangopadhyay, Somnath

    2016-02-01

    To estimate cost-effectiveness ratios and net costs of a training intervention to reduce morbidity among porters who carry loads without mechanical assistance in a developing country informal sector setting. Pre- and post-intervention survey data (n = 100) were collected in a prospective study: differences in physical/mental composite scores and pain scale scores were computed. Costs and economic benefits of the intervention were monetized with a net-cost model. Significant changes in physical composite scores (2.5), mental composite scores (3.2), and pain scale scores (-1.0) led to cost-effectiveness ratios of $6.97, $5.41, and $17.91, respectively. Multivariate analysis showed that program adherence enhanced effectiveness. The net cost of the intervention was -$5979.00 due to a reduction in absenteeism. Workplace ergonomic training is cost-effective and should be implemented wherein other engineering-control interventions are precluded due to infrastructural constraints.

  15. An integrated multi-criteria decision-making methodology for conveyor system selection

    Directory of Open Access Journals (Sweden)

    Pairat Jiamruangjarus

    2016-12-01

    Full Text Available Material handling equipment (MHE is important for every industry because it has an effect on the productivity of manufacturing. Conveyor systems are presently one popular type of MHE. This paper presents an integration of the analytic network process (ANP with the benefits, opportunities, costs and risk (BOCR model in order to select the best conveyor system. The proposed model established a network with four merits, six strategies criteria, and twenty six sub-criteria with four alternatives (present, roller conveyor, chain conveyor, and monorail. The ANP is to determine the relative weights of an evaluative criteria and decision alternatives. Therefore, the final ranking of the alternatives are calculated by synthesizing the score of each alternative under BOCR. The results showed that the best alternative under all five methods is the chain conveyor. These research results can be easily applied, adapted and used to improve performance of selecting the conveyer system in small and medium enterprises through large industries.

  16. Stochastic processes in cell biology

    CERN Document Server

    Bressloff, Paul C

    2014-01-01

    This book develops the theory of continuous and discrete stochastic processes within the context of cell biology.  A wide range of biological topics are covered including normal and anomalous diffusion in complex cellular environments, stochastic ion channels and excitable systems, stochastic calcium signaling, molecular motors, intracellular transport, signal transduction, bacterial chemotaxis, robustness in gene networks, genetic switches and oscillators, cell polarization, polymerization, cellular length control, and branching processes. The book also provides a pedagogical introduction to the theory of stochastic process – Fokker Planck equations, stochastic differential equations, master equations and jump Markov processes, diffusion approximations and the system size expansion, first passage time problems, stochastic hybrid systems, reaction-diffusion equations, exclusion processes, WKB methods, martingales and branching processes, stochastic calculus, and numerical methods.   This text is primarily...

  17. Genes involved in cell division in mycoplasmas

    Directory of Open Access Journals (Sweden)

    Frank Alarcón

    2007-01-01

    Full Text Available Bacterial cell division has been studied mainly in model systems such as Escherichia coli and Bacillus subtilis, where it is described as a complex process with the participation of a group of proteins which assemble into a multiprotein complex called the septal ring. Mycoplasmas are cell wall-less bacteria presenting a reduced genome. Thus, it was important to compare their genomes to analyze putative genes involved in cell division processes. The division and cell wall (dcw cluster, which in E. coli and B. subtilis is composed of 16 and 17 genes, respectively, is represented by only three to four genes in mycoplasmas. Even the most conserved protein, FtsZ, is not present in all mycoplasma genomes analyzed so far. A model for the FtsZ protein from Mycoplasma hyopneumoniae and Mycoplasma synoviae has been constructed. The conserved residues, essential for GTP/GDP binding, are present in FtsZ from both species. A strong conservation of hydrophobic amino acid patterns is observed, and is probably necessary for the structural stability of the protein when active. M. synoviae FtsZ presents an extended amino acid sequence at the C-terminal portion of the protein, which may participate in interactions with other still unknown proteins crucial for the cell division process.

  18. A mathematical model in cellular manufacturing system considering subcontracting approach under constraints

    Directory of Open Access Journals (Sweden)

    Kamran Forghani

    2012-10-01

    Full Text Available In this paper, a new mathematical model in cellular manufacturing systems (CMSs has been presented. In order to increase the performance of manufacturing system, the production quantity of parts has been considered as a decision variable, i.e. each part can be produced and outsourced, simultaneously. This extension would be minimized the unused capacity of machines. The exceptional elements (EEs are taken into account and would be totally outsourced to the external supplier in order to remove intercellular material handling cost. The problem has been formulated as a mixed-integer programming to minimize the sum of manufacturing variable costs under budget, machines capacity and demand constraints. Also, to evaluate advantages of the model, several illustrative numerical examples have been provided to compare the performance of the proposed model with the available classical approaches in the literature.

  19. Solving a mathematical model integrating unequal-area facilities layout and part scheduling in a cellular manufacturing system by a genetic algorithm.

    Science.gov (United States)

    Ebrahimi, Ahmad; Kia, Reza; Komijan, Alireza Rashidi

    2016-01-01

    In this article, a novel integrated mixed-integer nonlinear programming model is presented for designing a cellular manufacturing system (CMS) considering machine layout and part scheduling problems simultaneously as interrelated decisions. The integrated CMS model is formulated to incorporate several design features including part due date, material handling time, operation sequence, processing time, an intra-cell layout of unequal-area facilities, and part scheduling. The objective function is to minimize makespan, tardiness penalties, and material handling costs of inter-cell and intra-cell movements. Two numerical examples are solved by the Lingo software to illustrate the results obtained by the incorporated features. In order to assess the effects and importance of integration of machine layout and part scheduling in designing a CMS, two approaches, sequentially and concurrent are investigated and the improvement resulted from a concurrent approach is revealed. Also, due to the NP-hardness of the integrated model, an efficient genetic algorithm is designed. As a consequence, computational results of this study indicate that the best solutions found by GA are better than the solutions found by B&B in much less time for both sequential and concurrent approaches. Moreover, the comparisons between the objective function values (OFVs) obtained by sequential and concurrent approaches demonstrate that the OFV improvement is averagely around 17 % by GA and 14 % by B&B.

  20. Bioinspired assembly of small molecules in cell milieu.

    Science.gov (United States)

    Wang, Huaimin; Feng, Zhaoqianqi; Xu, Bing

    2017-05-09

    Self-assembly, the autonomous organization of components to form patterns or structures, is a prevalent process in nature at all scales. Particularly, biological systems offer remarkable examples of diverse structures (as well as building blocks) and processes resulting from self-assembly. The exploration of bioinspired assemblies not only allows for mimicking the structures of living systems, but it also leads to functions for applications in different fields that benefit humans. In the last several decades, efforts on understanding and controlling self-assembly of small molecules have produced a large library of candidates for developing the biomedical applications of assemblies of small molecules. Moreover, recent findings in biology have provided new insights on the assemblies of small molecules to modulate essential cellular processes (such as apoptosis). These observations indicate that the self-assembly of small molecules, as multifaceted entities and processes to interact with multiple proteins, can have profound biological impacts on cells. In this review, we illustrate that the generation of assemblies of small molecules in cell milieu with their interactions with multiple cellular proteins for regulating cellular processes can result in primary phenotypes, thus providing a fundamentally new molecular approach for controlling cell behavior. By discussing the correlation between molecular assemblies in nature and the assemblies of small molecules in cell milieu, illustrating the functions of the assemblies of small molecules, and summarizing some guiding principles, we hope this review will stimulate more molecular scientists to explore the bioinspired self-assembly of small molecules in cell milieu.

  1. Endocrine disrupting potentials of Bisphenol A, Bisphenol A dimethacrylate, 4-n-Nonyl-phenol and 4-Octylphenol assessed in cell model systems for effects on the estrogen-, androgen-, aryl hydrocarbon-receptor and aromatase activity

    DEFF Research Database (Denmark)

    Bonefeld-Jørgensen, Eva Cecilie; Long, Manhai; Hofmeister, Marlene V

    (RLU). Effects on the Androgen receptor (AR) trans-activation were investigated by co-transfection of the CHO-K1 hamster cells using the reporter vector MMTV-LUC and the AR expression vector pSVAR0. Effects on the CYP19 aromatase, an important enzyme in the steroid synthesis pathway involved...... of endogenous steroid metabolism potentially alters neuronal and reproductive system development: effects of environmental plasticizers. Program "Quality of Life and Management of Living Resources". (Contract no. QLK4-CT-2002-02637). http://endomet.bham.ac.uk                                                                                                                                                                                                                         ....

  2. Genesis Eco Systems, Inc. soil washing process

    International Nuclear Information System (INIS)

    Cena, R.J.

    1994-01-01

    The Genesis soil washing system is an integrated system of modular design allowing for maximum material handling capabilities, with optimized use of space for site mobility. The Surfactant Activated Bio-enhanced Remediation Equipment-Generation 1 (SABRE-1, Patent Applied For) modification was developed specifically for removing petroleum byproducts from contaminated soils. Scientifically formulated surfactants, introduced by high pressure spray nozzles, displace the contaminant from the surface of the soil particles into the process solution. Once the contaminant is dispersed into the liquid fraction of the process, it is either mechanically removed, chemically oxidized, or biologically oxidized. The contaminated process water is pumped through the Genesis Biosep (Patent Applied For) filtration system where the fines portion is flocculated, and the contaminant-rich liquid portion is combined with an activated mixture of nutrients and carefully selected bacteria to decompose the hydrocarbon fraction. The treated soil and dewatered fines are transferred to a bermed stockpile where bioremediation continues during drying. The process water is reclaimed, filtered, and recycled within the system

  3. A software platform to develop and execute kitting tasks on industrial cyber-physical systems

    DEFF Research Database (Denmark)

    Rovida, Francesco

    2017-01-01

    The current material handling infrastructure associated with manufacturing and assembly operations still register a great presence of human work for highly repetitive tasks. A major contributing factor for the low automation is that current manufacturing robots have little or no understanding...... the use of autonomous mobile manipulators to manage the industrial kitting operation, the task of collecting parts from the warehouse into kits for the production line. We discuss in particular the architectural and implementation aspects of a skill-based control platform that enables the mobile...... manipulators to accomplish their task autonomously and with a dynamic adaptation to the context. We show how the platform supports skill programming and configuration, maintains a world model with all relevant information to make autonomous task planning, interfaces with the Manufacturing Execution System (MES...

  4. Designing a mathematical model for integrating dynamic cellular manufacturing into supply chain system

    Science.gov (United States)

    Aalaei, Amin; Davoudpour, Hamid

    2012-11-01

    This article presents designing a new mathematical model for integrating dynamic cellular manufacturing into supply chain system with an extensive coverage of important manufacturing features consideration of multiple plants location, multi-markets allocation, multi-period planning horizons with demand and part mix variation, machine capacity, and the main constraints are demand of markets satisfaction in each period, machine availability, machine time-capacity, worker assignment, available time of worker, production volume for each plant and the amounts allocated to each market. The aim of the proposed model is to minimize holding and outsourcing costs, inter-cell material handling cost, external transportation cost, procurement & maintenance and overhead cost of machines, setup cost, reconfiguration cost of machines installation and removal, hiring, firing and salary worker costs. Aimed to prove the potential benefits of such a design, presented an example is shown using a proposed model.

  5. Feasibility of a super high energy density battery of the Li/BrF3 electrochemical system

    Science.gov (United States)

    Pyszczek, Michael F.; Ebel, Steven J.; Frysz, Christine A.

    To date, design and construction of a material handling and measurement system along with the apparatus required for waste material disposal has been completed. Preliminary corrosion screening of potential case materials is currently underway. A review of the literature, has led to the use of Monel (trademark) 400 as the material of construction for the handling and measurement system. The inherent stability of this material with bromine trifluoride in its liquid state is crucial to ensure that contamination does not occur during storage and handling. For applications which require a flexible or transparent material, items fabricated from perfluoroalkyoxy polymers (Teflon (trademark) PFA) were utilized. One such application encountered was in the design of the graduated tank which allows visual inspection of the material prior to dispensing. Containers used for compatibility/corrosion testing were also constructed of PFA.

  6. Systems

    International Nuclear Information System (INIS)

    Anon.

    1980-01-01

    Papers in this session describe the concept of mined geologic disposal system and methods for ensuring that the system, when developed, will meet all technical requirements. Also presented in the session are analyses of system parameters, such as cost and nuclear criticality potential, as well as a technical analysis of a requirement that the system permit retrieval of the waste for some period of time. The final paper discusses studies under way to investigate technical alternatives or complements to the mined geologic disposal system. Titles of the presented papers are: (1) Waste Isolation System; (2) Waste Isolation Economics; (3) BWIP Technical Baseline; (4) Criticality Considerations in Geologic Disposal of High-Level Waste; (5) Retrieving Nuclear Wastes from Repository; (6) NWTS Programs for the Evaluation of Technical Alternatives or Complements to Mined Geologic Repositories - Purpose and Objectives

  7. systems

    Directory of Open Access Journals (Sweden)

    Alexander Leonessa

    2000-01-01

    Full Text Available A nonlinear robust control-system design framework predicated on a hierarchical switching controller architecture parameterized over a set of moving nominal system equilibria is developed. Specifically, using equilibria-dependent Lyapunov functions, a hierarchical nonlinear robust control strategy is developed that robustly stabilizes a given nonlinear system over a prescribed range of system uncertainty by robustly stabilizing a collection of nonlinear controlled uncertain subsystems. The robust switching nonlinear controller architecture is designed based on a generalized (lower semicontinuous Lyapunov function obtained by minimizing a potential function over a given switching set induced by the parameterized nominal system equilibria. The proposed framework robustly stabilizes a compact positively invariant set of a given nonlinear uncertain dynamical system with structured parametric uncertainty. Finally, the efficacy of the proposed approach is demonstrated on a jet engine propulsion control problem with uncertain pressure-flow map data.

  8. Control of galactosylated glycoforms distribution in cell culture system.

    Science.gov (United States)

    McCracken, Neil A; Kowle, Ronald; Ouyang, Anli

    2014-01-01

    Cell culture process conditions including media components and bioreactor operation conditions have a profound impact on recombinant protein quality attributes. Considerable changes in the distribution of galactosylated glycoforms (G0F, G1F, and G2F) were observed across multiple CHO derived recombinant proteins in development at Eli Lilly and Company when switching to a new chemically defined (CD) media platform condition. In the new CD platform, significantly lower G0F percentages and higher G1F and G2F were observed. These changes were of interest as glycosylation heterogeneity can impact the effectiveness of a protein. A systematic investigation was done to understand the root cause of the change and control strategy for galactosylated glycoforms distribution. It was found that changes in asparagine concentration could result in a corresponding change in G0F, G1F, and G2F distribution. A follow-up study examined a wider range of asparagine concentration and it was found that G0F, G1F, and G2F percentage could be titrated by adjusting asparagine concentration. The observed changes in heterogeneity from changing asparagine concentration are due to resulting changes in ammonium metabolism. Further study ascertained that different integrated ammonium level during the cell culture process could control G0F, G1F, and G2F percentage distribution. A mechanism hypothesis is proposed that integrated ammonium level impacts intracellular pH, which further regulates β-1, 4 galactosyltransferase activity. © 2014 American Institute of Chemical Engineers.

  9. Effect of Nanoparticles on Complement System in Cell Culture Model

    National Research Council Canada - National Science Library

    Sladowski, Dariusz T

    2006-01-01

    ... Corporation San Diego California, Different sizes of nanoparticles such as silver (Ag; 151,000 nm) molybdenum (MoO3; 30 150 nm), aluminum (Al; 30 103 nm), iron oxide (Fe3O4; 30, 47 nm) and titanium dioxide...

  10. Advancement in fuel handling system of PREFRE-2, Tarapur

    International Nuclear Information System (INIS)

    Agarwal, K.; Manole, A.A.; Datir, K.A.

    2015-01-01

    Utilising the experience already generated in various aspects of reprocessing technology such as process chemistry, engineering and automation, instrumentation and control, PREFRE-2 has been built with latest technology for reprocessing of 220 MW PHWR spent fuel from northern region. A high level of automation in handling and remote maintenance concepts have been used for most of the process equipment. The Head End System involves Spent Fuel Transportation, Receipt, Handling, Storage, Charging, Chopping of fuel and hull disposal. Besides these basic functions, material handling, remote handling, viewing, remote operation and maintenance of related equipment are also involved. In order to meet the rated plant capacity, system reliability and automation were the foremost criteria while designing the systems. As PREFRE-2 fuel handling system utilizes existing facilities of PREFRE for fuel receipt and storage, design of new systems and modifications/up-gradation in existing facilities were done to improve efficiency of head end system. This paper gives outline of the advancements made in fuel handling system of PREFRE-2, Tarapur

  11. Mockup of an automated material transport system for remote handling

    International Nuclear Information System (INIS)

    Porter, M.L.

    1992-01-01

    An Automated Material Transport System (AMTS) was identified for transport of samples within a Material and Process Control Laboratory (MPCL). The MPCL was designed with a dry sample handling laboratory and a wet chemistry analysis laboratory. Each laboratory contained several processing gloveboxes. The function of the AMTS was to automate the handling of materials, multiple process samples, and bulky items between process stations with a minimum of operator intervention and with minimum o[ waiting periods and nonproductive activities. This paper discusses the system design features, capabilities and results of initial testing. The overall performance of the AMTS is very good. No major problems or concerns were identified. System commands are simple and logical making the system user friendly. Operating principle and design of individual components is simple. With the addition of various track modules, the system can be configured in most any configuration. The AMTS lends itself very well for integration with other automated systems or products. The AMTS is suited for applications involving light payloads which require multiple sample and material handling, lot tracking, and system integration with other products

  12. Electron Microscopy of Nanostructures in Cells

    DEFF Research Database (Denmark)

    Købler, Carsten

    with cells is therefore increasingly more relevant from both an engineering and a toxicological viewpoint. My work involves developing and exploring electron microscopy (EM) for imaging nanostructures in cells, for the purpose of understanding nanostructure-cell interactions in terms of their possibilities...

  13. Modeling cell-in-cell structure into its biological significance

    OpenAIRE

    He, M-f; Wang, S; Wang, Y; Wang, X-n

    2013-01-01

    Although cell-in-cell structure was noted 100 years ago, the molecular mechanisms of ?entering' and the destination of cell-in-cell remain largely unclear. It takes place among the same type of cells (homotypic cell-in-cell) or different types of cells (heterotypic cell-in-cell). Cell-in-cell formation affects both effector cells and their host cells in multiple aspects, while cell-in-cell death is under more intensive investigation. Given that cell-in-cell has an important role in maintainin...

  14. systems

    Directory of Open Access Journals (Sweden)

    Patrick L. Brockett

    1978-01-01

    Full Text Available Suppose S={{Xnj,   j=1,2,…,kn}} is an infinitesimal system of random variables whose centered sums converge in law to a (necessarily infinitely divisible distribution with Levy representation determined by the triple (γ,σ2,M. If {Yj,   j=1,2,…} are independent indentically distributed random variables independent of S, then the system S′={{YjXnj,j=1,2,…,kn}} is obtained by randomizing the scale parameters in S according to the distribution of Y1. We give sufficient conditions on the distribution of Y in terms of an index of convergence of S, to insure that centered sums from S′ be convergent. If such sums converge to a distribution determined by (γ′,(σ′2,Λ, then the exact relationship between (γ,σ2,M and (γ′,(σ′2,Λ is established. Also investigated is when limit distributions from S and S′ are of the same type, and conditions insuring products of random variables belong to the domain of attraction of a stable law.

  15. Development of a telerobotic system for handling contaminated process equipment

    International Nuclear Information System (INIS)

    Fisher, J.J.; Ward, C.R.; Schuler, T.F.

    1987-01-01

    E. I. du Pont de Nemours and Company is evaluating a unique eight-degree-of-freedom Telerobot manipulator to perform size-reduction and material handling operations on contaminated process equipment at the Savannah River Plant (SRP). The Telerobot will be installed in the proposed Transuranic (TRU) Waste Processing Facility, which is scheduled to be operational by 1990. A full-scale prototype Telerobot, manufactured by GCA Corporation, St. Paul, MN is being tested with other process equipment in the Components Test Facility at the Savannah River Laboratory (SRL). All telerobotic operations required in the TRU Waste Facility such as crate unpacking, equipment dismantling, material size-reduction, and selected maintenance operations are being tested. This paper discusses the major mechanical and control features of the Telerobot system. Several system enhancements were added by SRL, including a new quick-hand-change coupling and expanded software control functions. The new software enables a system operator to perform both teleoperated and automatic tasks through several operating modes. These enhancements, as well as future mechanical, control system, and software features, are reviewed

  16. SYSTEM

    Directory of Open Access Journals (Sweden)

    K. Swarnalatha

    2013-01-01

    Full Text Available Risk analysis of urban aquatic systems due to heavy metals turns significant due to their peculiar properties viz. persis tence, non-degradab ility, toxicity, and accumulation. Akkulam Veli (AV, an urba n tropical lake in south India is subjected to various environmental stresses due to multiple waste discharge, sand mining, developmental activities, tour ism related activitie s etc. Hence, a comprehensive approach is adopted for risk assessment using modified degree of contamination factor, toxicity units based on numerical sediment quality guidelines (SQGs, and potentialecological risk indices. The study revealed the presence of toxic metals such as Cr, C d, Pb and As and the lake is rated under ‘low ecological risk’ category.

  17. Cell-in-Cell Death Is Not Restricted by Caspase-3 Deficiency in MCF-7 Cells

    Science.gov (United States)

    Wang, Shan; He, Meifang; Li, Linmei; Liang, Zhihua; Zou, Zehong

    2016-01-01

    Purpose Cell-in-cell structures are created by one living cell entering another homotypic or heterotypic living cell, which usually leads to the death of the internalized cell, specifically through caspase-dependent cell death (emperitosis) or lysosome-dependent cell death (entosis). Although entosis has attracted great attention, its occurrence is controversial, because one cell line used in its study (MCF-7) is deficient in caspase-3. Methods We investigated this issue using MCF-7 and A431 cell lines, which often display cell-in-cell invasion, and have different levels of caspase-3 expression. Cell-in-cell death morphology, microstructures, and signaling pathways were compared in the two cell lines. Results Our results confirmed that MCF-7 cells are caspase-3 deficient with a partial deletion in the CASP-3 gene. These cells underwent cell death that lacked typical apoptotic properties after staurosporine treatment, whereas caspase-3-sufficient A431 cells displayed typical apoptosis. The presence of caspase-3 was related neither to the lysosome-dependent nor to the caspase-dependent cell-in-cell death pathway. However, the existence of caspase-3 was associated with a switch from lysosome-dependent cell-in-cell death to the apoptotic cell-in-cell death pathway during entosis. Moreover, cellular hypoxia, mitochondrial swelling, release of cytochrome C, and autophagy were observed in internalized cells during entosis. Conclusion The occurrence of caspase-independent entosis is not a cell-specific process. In addition, entosis actually represents a cellular self-repair system, functioning through autophagy, to degrade damaged mitochondria resulting from cellular hypoxia in cell-in-cell structures. However, sustained autophagy-associated signal activation, without reduction in cellular hypoxia, eventually leads to lysosome-dependent intracellular cell death. PMID:27721872

  18. Selecting a pharmacy layout design using a weighted scoring system.

    Science.gov (United States)

    McDowell, Alissa L; Huang, Yu-Li

    2012-05-01

    A weighted scoring system was used to select a pharmacy layout redesign. Facilities layout design techniques were applied at a local hospital pharmacy using a step-by-step design process. The process involved observing and analyzing the current situation, observing the current available space, completing activity flow charts of the pharmacy processes, completing communication and material relationship charts to detail which areas in the pharmacy were related to one another and how they were related, researching applications in other pharmacies or in scholarly works that could be beneficial, numerically defining space requirements for areas within the pharmacy, measuring the available space within the pharmacy, developing a set of preliminary designs, and modifying preliminary designs so they were all acceptable to the pharmacy staff. To select a final layout that could be implemented in the pharmacy, those layouts were compared via a weighted scoring system. The weighted aspect further allowed additional emphasis on categories based on their effect on pharmacy performance. The results produced a beneficial layout design as determined through simulated models of the pharmacy operation that more effectively allocated and strategically located space to improve transportation distances and materials handling, employee utilization, and ergonomics. Facilities layout designs for a hospital pharmacy were evaluated using a weighted scoring system to identify a design that was superior to both the current layout and alternative layouts in terms of feasibility, cost, patient safety, employee safety, flexibility, robustness, transportation distance, employee utilization, objective adherence, maintainability, usability, and environmental impact.

  19. Accelerating Acceptance of Fuel Cell Backup Power Systems - Final Report

    Energy Technology Data Exchange (ETDEWEB)

    Petrecky, James; Ashley, Christopher

    2014-07-21

    Since 2001, Plug Power has installed more than 800 stationary fuel cell systems worldwide. Plug Power’s prime power systems have produced approximately 6.5 million kilowatt hours of electricity and have accumulated more than 2.5 million operating hours. Intermittent, or backup, power products have been deployed with telecommunications carriers and government and utility customers in North and South America, Europe, the United Kingdom, Japan and South Africa. Some of the largest material handling operations in North America are currently using the company’s motive power units in fuel cell-powered forklifts for their warehouses, distribution centers and manufacturing facilities. The low-temperature GenSys fuel cell system provides remote, off-grid and primary power where grid power is unreliable or nonexistent. Built reliable and designed rugged, low- temperature GenSys delivers continuous or backup power through even the most extreme conditions. Coupled with high-efficiency ratings, low-temperature GenSys reduces operating costs making it an economical solution for prime power requirements. Currently, field trials at telecommunication and industrial sites across the globe are proving the advantages of fuel cells—lower maintenance, fuel costs and emissions, as well as longer life—compared with traditional internal combustion engines.

  20. Where to Go: Breaking the Symmetry in Cell Motility

    Science.gov (United States)

    2016-01-01

    Cell migration in the “correct” direction is pivotal for many biological processes. Although most work is devoted to its molecular mechanisms, the cell’s preference for one direction over others, thus overcoming intrinsic random motility, epitomizes a profound principle that underlies all complex systems: the choice of one axis, in structure or motion, from a uniform or symmetric set of options. Explaining directional motility by an external chemo-attractant gradient does not solve but only shifts the problem of causation: whence the gradient? A new study in PLOS Biology shows cell migration in a self-generated gradient, offering an opportunity to take a broader look at the old dualism of extrinsic instruction versus intrinsic symmetry-breaking in cell biology. PMID:27196433

  1. Machine learning applications in cell image analysis.

    Science.gov (United States)

    Kan, Andrey

    2017-07-01

    Machine learning (ML) refers to a set of automatic pattern recognition methods that have been successfully applied across various problem domains, including biomedical image analysis. This review focuses on ML applications for image analysis in light microscopy experiments with typical tasks of segmenting and tracking individual cells, and modelling of reconstructed lineage trees. After describing a typical image analysis pipeline and highlighting challenges of automatic analysis (for example, variability in cell morphology, tracking in presence of clutters) this review gives a brief historical outlook of ML, followed by basic concepts and definitions required for understanding examples. This article then presents several example applications at various image processing stages, including the use of supervised learning methods for improving cell segmentation, and the application of active learning for tracking. The review concludes with remarks on parameter setting and future directions.

  2. 78 FR 46363 - Notice of Availability of the Draft Supplemental Environmental Impact Statement for the Palen...

    Science.gov (United States)

    2013-07-31

    ... resources, water resources, geological resources and hazards, hazardous materials handling, noise..., reliability, transmission system engineering and transmission line safety and nuisance. Before including your...

  3. 76 FR 66079 - Notice of Intent To Prepare a Supplemental Draft Environmental Impact Statement for the K Road...

    Science.gov (United States)

    2011-10-25

    ... resources, water resources, geological resources and hazards, hazardous materials handling, noise..., efficiency, reliability, transmission system engineering and transmission line safety and nuisance. If the...

  4. 76 FR 54483 - Notice of Intent To Prepare a Supplemental Environmental Impact Statement and a Resource...

    Science.gov (United States)

    2011-09-01

    ..., hazardous materials handling, land and airspace use, noise, paleontological resources, public health..., transmission-system engineering, transmission line safety, and nuisance issues. By this notice, the BLM is...

  5. In-cell maintenance by manipulator arm with 3D workspace information recreated by laser rangefinder

    International Nuclear Information System (INIS)

    Kitamura, Akihiro; Nakai, Koji; Namekawa, Takashi; Watahiki, Masatoshi

    2011-01-01

    Highlights: → We developed a remote control system for maintenance of in-cell type fuel fabrication equipment. → The system display recreated three-dimensional information of the workspace from data obtained by laser rangefinder and conventional cameras. It has allowed us to operate a manipulator arm remotely with several control modes. → We implemented remote handling experiments using mock up equipment. Performance was compared for remote operation conducted using several different display and operation modes. → It was observed that integration of 3D information from the laser rangefinder reduced operation time and reinforced visual information during remote operation. - Abstract: We developed a remote control system for maintenance of in-cell type fuel fabrication equipment. The system display recreated three-dimensional information of the workspace from data obtained by laser rangefinder and conventional cameras. It has allowed us to operate a manipulator arm remotely with several control modes. In order to evaluate the effectiveness and usefulness of developed system, we implemented remote handling experiments using mock up equipment. Performance was compared for remote operation conducted using several different display and operation modes. We confirmed that the system is able to maintain in-cell fuel fabrication equipment in each display and operation mode. Times required to complete the remote operations were collected and compared in each mode. It was observed that integration of 3D information from the laser rangefinder reduced operation time and reinforced visual information during remote operation.

  6. Phosphoinositide turnover in cell growth and transformation

    International Nuclear Information System (INIS)

    Fleischman, L.F.

    1987-01-01

    Interaction of cells with various stimuli triggers a common signal transduction pathway involving breakdown and resynthesis of the minor membrane lipid phosphatidylinositol-4,5-bisphosphate (PIP 2 ). Hydrolysis of PIP 2 by phospholipase C generates two key catabolites-inositol-1,4,5-trisphosphate (IP 3 ) and diacylglycerol (DAG)-which mediate and amplify cellular responses. These studies provide evidence for potential involvement of this pathway in oncogenic transformation and cell cycle progression. Altered levels of PIP 2 and its breakdown products were found in cells transformed by ras oncogenes, in contrast to untransformed counterparts. Steady-state levels of PIP 2 , DAG and inositol phosphates were measured in NIH 3T3 and NRK cells metabolically labelled with 3 H-glycerol and 3 H-inositol. DAG and inositol phosphate levels were significantly elevated by 2.5-3 fold in the transformed cells while levels of PIP 2 were decreased. These findings suggest that the ras protein may activate phospholipase C. Elevated DAG content in the transformed cells was also measured by phosphorylation of DAG using a partially purified DAG kinase, indicating that the differences seen could not be attributed to differences in labelling between the cell lines

  7. Interdisciplinary Team Science in Cell Biology.

    Science.gov (United States)

    Horwitz, Rick

    2016-11-01

    The cell is complex. With its multitude of components, spatial-temporal character, and gene expression diversity, it is challenging to comprehend the cell as an integrated system and to develop models that predict its behaviors. I suggest an approach to address this issue, involving system level data analysis, large scale team science, and philanthropy. Copyright © 2016 Elsevier Ltd. All rights reserved.

  8. Retrieval-travel-time model for free-fall-flow-rack automated storage and retrieval system

    Science.gov (United States)

    Metahri, Dhiyaeddine; Hachemi, Khalid

    2018-03-01

    Automated storage and retrieval systems (AS/RSs) are material handling systems that are frequently used in manufacturing and distribution centers. The modelling of the retrieval-travel time of an AS/RS (expected product delivery time) is practically important, because it allows us to evaluate and improve the system throughput. The free-fall-flow-rack AS/RS has emerged as a new technology for drug distribution. This system is a new variation of flow-rack AS/RS that uses an operator or a single machine for storage operations, and uses a combination between the free-fall movement and a transport conveyor for retrieval operations. The main contribution of this paper is to develop an analytical model of the expected retrieval-travel time for the free-fall flow-rack under a dedicated storage assignment policy. The proposed model, which is based on a continuous approach, is compared for accuracy, via simulation, with discrete model. The obtained results show that the maximum deviation between the continuous model and the simulation is less than 5%, which shows the accuracy of our model to estimate the retrieval time. The analytical model is useful to optimise the dimensions of the rack, assess the system throughput, and evaluate different storage policies.

  9. Impact of repetitive manual materials handling and psychosocial work factors on the future prevalence of chronic low-back pain among construction workers.

    Science.gov (United States)

    Latza, Ute; Pfahlberg, Annette; Gefeller, Olaf

    2002-10-01

    This study investigated the influence of manual stone and brick handling and psychosocial work factors on the risk of chronic low-back pain and describes the impact in terms of risk advancement period. The Hamburg Construction Worker Study included a longitudinal study among 488 male construction workers. Adjusted prevalence ratios (PR) of chronic low-back pain (>3 months during the last 12 months) according to self-reported worktasks in the baseline survey were estimated with the Cox proportional hazards model. The 1-year prevalence of chronic low-back pain was 15.4%. Workers with chronic low-back pain in the baseline survey had a higher risk of such pain during the follow-up (PR4.07,95% CI 2.18-7.59). The prevalence in association with laying large lime sandstones for >2 hours per shift (PR 1.80, 95% CI 1.04-3.14) further increased after adjustment for job category (PR 2.69, 95% CI 1.25-5.78), and it advanced the risk by a risk advancement period of 18 years (95% CI 4-39). Workers with low satisfaction with their work achievements had a higher prevalence of chronic low-back pain (PR 2.07, 95% CI 1.10-3.88). Similar risk estimates were observed in the subgroup without chronic low-back pain in the baseline survey. A strong effect of time pressure wasonly present for these workers (high: P R 6.30,95% CI 1.41-28.21). The results suggest that repetitive work involving bent positions and the manual manipulation of heavy stones increases the risk of future chronic low-back pain. For risk communication, the notion that a 40-year-old construction worker laying large sandstones has the same risk as an unexposed 58-year-old construction worker may be more informative.

  10. Manual materials handling in mining: the effect of rod heights and foot positions when lifting "in-the-hole" drill rods.

    Science.gov (United States)

    Plamondon, André; Delisle, Alain; Trimble, Karin; Desjardins, Pierre; Rickwood, Trevor

    2006-11-01

    There is a paucity of studies focusing on the lifting of rods or long awkward heavy objects. In-the-hole (ITH) drilling is a heavy repetitive mining task, which has been identified as having a relatively high incidence and severity rate of musculoskeletal injuries. The purpose of this study was to examine how the load experienced by ITH drill operators changed when lifting a vertical drilling rod (1.61m, 35kg) using two rod heights and four different foot positions. In addition, a symmetrical lift with a lifting index (LI) of 1.4 also served as a comparison to determine possible risk of low back injury. Eleven experienced ITH drill operators participated in the study. Each subject was required to lift a vertical drilling rod until the upper body was in an erect posture using four different foot positions (0 degrees =subject facing the rod, 45 degrees =subject oblique to the rod, 90 degrees =subject right side to the rod and freestyle). In addition, two rod height conditions were studied where the base of the vertical rod was supported either (1) at ground level (height of rod CG=0.83m) or (2) on a 20cm rack (height of rod CG=1.03m). Finally, each subject lifted a 21.5kg box in the sagittal plane, which corresponded to an LI of 1.4 in the NIOSH lifting equation. Reflective markers were placed on the subjects, and three video cameras and one force plate were used to record the forces and the motion of the subjects' segments. Two surface electrodes were applied on the right and the left erector spinae (ES) at the level of L3. Back loading was defined by the level of the peak moments, the mechanical work and erector spinae muscle activity (EMG). It was found that the vertical height of the rod had the most significant impact on back loading, while the effect of the initial foot positioning relative to the rod was limited by the technique adopted by the drillers. Moreover, it was found that some of the subjects used techniques less strenuous for the back than others. Finally, the asymmetrical lifting component was found to be the most negative aspect of lifting an ITH drill rod compared to a standard symmetrical lift (NIOSH).

  11. Assessment of manual material handling using Iranian MMH regulations and comparison with NIOSH equation and MAC method in one of the metal casting industries in Tehran, 2011

    Directory of Open Access Journals (Sweden)

    2013-08-01

    Result: Results showed an acceptable agreement between Iranian regulation and MAC method as well as Iranian regulation and NIOSH equation. However, no acceptable agreement was observed between MAC method and NIOSH equation.

  12. Perancangan Material Handling Equipment Pada Proses Penggilingan Ke Oksidasi Enzimatis Bubuk Teh Menggunakan Metode Perancangan Produk Rasional Pada PT Perkebunan Nusantara VIII Rancabali

    OpenAIRE

    Lestari, Imelia Rizki; Anugraha, Rino Andias; Iqbal, Muhammad

    2016-01-01

    PT Perkebunan Nusantara VIII Rancabali merupakan salah satu Perusahaan pengolahan teh hitam ortodoks di Indonesia yang menghasilkan dua belas jenis teh hitam untuk dijual di dalam negeri maupun diekspor ke beberapa negara. Kualitas teh yang dihasilkan oleh Perusahaan ini tentunya akan mempengaruhi harga teh yang akan dijual. Dalam proses pengolahan teh hitam ortodoks, proses oksidasi enzimatis merupakan proses yang paling menentukan kualitas bubuk teh yang akan dihasilkan nantinya, sehingga d...

  13. REMOTE IN-CELL SAMPLING IMPROVEMENTS PROGRAM AT THESAVANNAH RIVER SITE (SRS) DEFENSE WASTE PROCESSING FACILITY (DWPF)

    International Nuclear Information System (INIS)

    Marzolf, A

    2007-01-01

    Remote Systems Engineering (RSE) of the Savannah River National Lab (SRNL) in combination with the Defense Waste Processing Facility(DWPF) Engineering and Operations has evaluated the existing equipment and processes used in the facility sample cells for 'pulling' samples from the radioactive waste stream and performing equipment in-cell repairs/replacements. RSE has designed and tested equipment for improving remote in-cell sampling evolutions and reducing the time required for in-cell maintenance of existing equipment. The equipment within the present process tank sampling system has been in constant use since the facility start-up over 17 years ago. At present, the method for taking samples within the sample cells produces excessive maintenance and downtime due to frequent failures relative to the sampling station equipment and manipulator. Location and orientation of many sampling stations within the sample cells is not conducive to manipulator operation. The overextension of manipulators required to perform many in-cell operations is a major cause of manipulator failures. To improve sampling operations and reduce downtime due to equipment maintenance, a Portable Sampling Station (PSS), wireless in-cell cameras, and new commercially available sampling technology has been designed, developed and/or adapted and tested. The uniqueness of the design(s), the results of the scoping tests, and the benefits relative to in-cell operation and reduction of waste are presented

  14. About novelty of radiation drug 'Storm in cells'

    International Nuclear Information System (INIS)

    Korchubekov, B.

    2005-01-01

    Drugs 'Storm in cells' is intended for treatment of infection wounds and burns in medical practice. The preparation represents the electro- activated mixture consisting uranium and thorium masses in the mumie base, table salt, activated carbon and water. Advantage of the drug 'Storm in cells' in comparison with prototype is increase of wound and burns repair effectiveness in 10-11 %

  15. Standard guide for mechanical drive systems for remote operation in hot cell facilities

    CERN Document Server

    American Society for Testing and Materials. Philadelphia

    2010-01-01

    1.1 Intent: 1.1.1 The intent of this standard is to provide general guidelines for the design, selection, quality assurance, installation, operation, and maintenance of mechanical drive systems used in remote hot cell environments. The term mechanical drive systems used herein, encompasses all individual components used for imparting motion to equipment systems, subsystems, assemblies, and other components. It also includes complete positioning systems and individual units that provide motive power and any position indicators necessary to monitor the motion. 1.2 Applicability: 1.2.1 This standard is intended to be applicable to equipment used under one or more of the following conditions: 1.2.1.1 The materials handled or processed constitute a significant radiation hazard to man or to the environment. 1.2.1.2 The equipment will generally be used over a long-term life cycle (for example, in excess of two years), but equipment intended for use over a shorter life cycle is not excluded. 1.2.1.3 The ...

  16. Impact of Flexibility of Manufacturing System Components on Competitiveness of SMEs in Northern India

    Directory of Open Access Journals (Sweden)

    Shakun Preet Kaur

    2016-01-01

    Full Text Available The present manufacturing environment is characterized by a number of changes which poses challenges to a typical manufacturing unit. Time demands a shift from the traditional manufacturing strategies as they do not fit to present market competition. A flexible systems strategy has to be designed for remaining competitive in the market and perform well. For designing strategies and policies it is important to know the factors that influence performance of the system. The aim of this paper is to assess the impact of the flexibility of manufacturing system components on competitiveness of SMEs in northern India. A questionnaire based survey was conducted in the SMEs across northern India analysing three sectors namely automotive, machine tool and light engineering (mechanical components and equipment. The study contributes to the existing literature by empirically investigating the impact of machine, material handling and worker flexibility on competitiveness of manufacturing firms. This paper presents a Structural Equation Model displaying the impact of flexibility of manufacturing system components on competitiveness of SMEs.

  17. Safeguards systems analysis research and development and the practice of safeguards at DOE facilities

    International Nuclear Information System (INIS)

    Zack, N.R.; Thomas, K.E.; Markin, J.T.; Tape, J.W.

    1991-01-01

    Los Alamos Safeguards Systems Group personnel interact with Department of Energy (DOE) nuclear materials processing facilities in a number of ways. Among them are training courses, formal technical assistance such as developing information management or data analysis software, and informal ad hoc assistance especially in reviewing and commenting on existing facility safeguards technology and procedures. These activities are supported by the DOE Office of Safeguards and Security, DOE Operations Offices, and contractor organizations. Because of the relationships with the Operations Office and facility personnel, the Safeguards Systems Group research and development (R and D) staff have developed an understanding of the needs of the entire complex. Improved safeguards are needed in areas such as materials control activities, accountability procedures and techniques, systems analysis and evaluation methods, and material handling procedures. This paper surveys the generic needs for efficient and cost effective enhancements in safeguards technologies and procedures at DOE facilities, identifies areas where existing safeguards R and D products are being applied or could be applied, and sets a direction for future systems analysis R and D to address practical facility safeguards needs

  18. GPU Acceleration of Particle-In-Cell Methods

    Science.gov (United States)

    Cowan, Benjamin; Averkin, Sergey; Cary, John; Leddy, Jarrod; Sides, Scott; Werner, Gregory

    2017-10-01

    Graphics processing units (GPUs) have become key components in many supercomputing systems, as they can provide more computations relative to their cost and power consumption than conventional processors. However, to take full advantage of this capability, they require a strict programming model which involves single-instruction multiple-data execution as well as significant constraints on memory access. To bring the full power of GPUs to bear on plasma physics problems, we must adapt the computational methods to this new programming model. We have developed a GPU implementation of the particle-in-cell (PIC) method, one of the mainstays of plasma physics simulation. This framework is highly general and enables advanced PIC features such as high order particles and absorbing boundary conditions. The main elements of the PIC loop, including field interpolation and particle deposition, are designed to optimize memory access. We describe recent progress in these algorithms, including arbitrary grid types and multiple GPUs per node. Work supported by DARPA Contract No. W31P4Q-16-C-0009.

  19. Second order gyrokinetic theory for particle-in-cell codes

    Science.gov (United States)

    Tronko, Natalia; Bottino, Alberto; Sonnendrücker, Eric

    2016-08-01

    The main idea of the gyrokinetic dynamical reduction consists in a systematical removal of the fast scale motion (the gyromotion) from the dynamics of the plasma, resulting in a considerable simplification and a significant gain of computational time. The gyrokinetic Maxwell-Vlasov equations are nowadays implemented in for modeling (both laboratory and astrophysical) strongly magnetized plasmas. Different versions of the reduced set of equations exist, depending on the construction of the gyrokinetic reduction procedure and the approximations performed in the derivation. The purpose of this article is to explicitly show the connection between the general second order gyrokinetic Maxwell-Vlasov system issued from the modern gyrokinetic theory and the model currently implemented in the global electromagnetic Particle-in-Cell code ORB5. Necessary information about the modern gyrokinetic formalism is given together with the consistent derivation of the gyrokinetic Maxwell-Vlasov equations from first principles. The variational formulation of the dynamics is used to obtain the corresponding energy conservation law, which in turn is used for the verification of energy conservation diagnostics currently implemented in ORB5. This work fits within the context of the code verification project VeriGyro currently run at IPP Max-Planck Institut in collaboration with others European institutions.

  20. Unified changes in cell size permit coordinated leaf evolution.

    Science.gov (United States)

    Brodribb, Tim J; Jordan, Greg J; Carpenter, Raymond J

    2013-07-01

    The processes by which the functions of interdependent tissues are coordinated as lineages diversify are poorly understood. Here, we examine evolutionary coordination of vascular, epidermal and cortical leaf tissues in the anatomically, ecologically and morphologically diverse woody plant family Proteaceae. We found that, across the phylogenetic range of Proteaceae, the sizes of guard, epidermal, palisade and xylem cells were positively correlated with each other but negatively associated with vein and stomatal densities. The link between venation and stomata resulted in a highly efficient match between potential maximum water loss (determined by stomatal conductance) and the leaf vascular system's capacity to replace that water. This important linkage is likely to be driven by stomatal size, because spatial limits in the packing of stomata onto the leaf surface apparently constrain the maximum size and density of stomata. We conclude that unified evolutionary changes in cell sizes of independent tissues, possibly mediated by changes in genome size, provide a means of substantially modifying leaf function while maintaining important functional links between leaf tissues. Our data also imply the presence of alternative evolutionary strategies involving cellular miniaturization during radiation into closed forest, and cell size increase in open habitats. © 2013 The Authors. New Phytologist © 2013 New Phytologist Trust.

  1. An accountancy system for nuclear materials control in research centres

    International Nuclear Information System (INIS)

    Buttler, R.; Bueker, H.; Vallee, J.

    1979-01-01

    The Nuclear Accountancy and Control System (NACS) was developed at KFA Juelich in accordance with the requirements of the Non-Proliferation Treaty. The main features are (1) recording of nuclear material in inventory items. These are combined to form batches wherever suitable; (2) extrapolation of accounting data as a replacement for detailed measurement of inventory items data. Recording and control of nuclear material are carried out on two levels with access to a common data bank. The lower level deals with nuclear materials handling plus internal management while on the upper level there is a central control point which is responsible for nuclear safeguarding within the entire research centre. By keeping the organizational and technical infrastructure it was possible to develop a system which is both economical and operator-oriented. In this system the emphasis of nuclear safeguarding is placed on the acquisition of the nuclear material inventory. As much consideration has been given to the interests of the various operational levels and organizational units as to internal and national regulations. Since it is part of the safeguarding and control system, access to the NACS must be restricted to a limited number of users only. Furthermore, it must include facilities for manual control in the form of records. Authorization for access must correspond with the various tasks of different user groups. All necessary data are acquired decentrally in the organizational units and entered via a terminal. It is available to the user groups on both levels through a central data bank. To meet all requirements, the NACS has been designed as an integrated, computer-assisted information system for the automated processing of extensive and multi-level nuclear materials data. As part of the preventive measures entailed with nuclear safeguarding, the accountancy system enables the operator of a nuclear plant to furnish proof of non-diversion of nuclear material. (author)

  2. Transient processes in cell proliferation kinetics

    CERN Document Server

    Yakovlev, Andrej Yu

    1989-01-01

    A mathematician who has taken the romantic decision to devote himself to biology will doubtlessly look upon cell kinetics as the most simple and natural field of application for his knowledge and skills. Indeed, the thesaurus he is to master is not so complicated as, say, in molecular biology, the structural elements of the system, i. e. ceils, have been segregated by Nature itself, simple considerations of balance may be used for deducing basic equations, and numerous analogies in other areas of science also superficial add to one"s confidence. Generally speaking, this number of impression is correct, as evidenced by the very great theoretical studies on population kinetics, unmatched in other branches of mathematical biology. This, however, does not mean that mathematical theory of cell systems has traversed in its development a pathway free of difficulties or errors. The seeming ease of formalizing the phenomena of cell kinetics not infrequently led to the appearance of mathematical models lacking in adequ...

  3. Cost of radon-barrier systems for uranium mill tailings

    International Nuclear Information System (INIS)

    Baker, E.G.; Hartley, J.N.

    1982-08-01

    This report deals specifically with the cost of three types of radon barrier systems, earthen covers, asphalt emulsion covers, and multilayer covers, which could meet standards proposed by the Environmental Protection Agency to stabilize uranium mill tailings located primarily in the western US. In addition, the report includes a sensitivity analysis of various factors which significantly effect the overall cost of the three systems. These analyses were based on a generic disposal site. Four different 3m thick earthen covers were tested and cost an average of $27/m 2 . The least expensive earthen cover cost was about $21/m 2 . The asphalt cover system (6 to 7 cm of asphalt topped with 0.6m of overburden) cost about $28/m 2 . The four multilayer covers averaged $57/m 2 , but materials handling problems encountered during the test inflated this cost above what was anticipated and significant cost reductions should be possible. The least expensive multilayer cover cost $43/m 2 . Based on the results of the Grand Junction field test we estimated the cost of covering the tailings from three high priority sites, Durango, Shiprock, and Salt Lake City (Vitro). The cost of a 3m earthen cover ranged from $18 to 33/m 2 for the seven disposal sites (two or three at each location) studied. The cost of asphalt cover systems were $23 to 28/m 2 and the multilayer cover costs were between $31 to 36/m 2 . The earthen cover costs are less than the Grand Junction field test cost primarily because cover material is available at or near most of the disposal sites selected. Earthen material was imported from 6 to 10 miles for the field test. Assuming more efficienct utilization of materials significantly reduced the cost of the multilayer covers

  4. Safety of Hydrogen Systems Installed in Outdoor Enclosures

    Energy Technology Data Exchange (ETDEWEB)

    Barilo, Nick F. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States)

    2013-11-01

    The Hydrogen Safety Panel brings a broad cross-section of expertise from the industrial, government, and academic sectors to help advise the U.S. Department of Energy’s (DOE) Fuel Cell Technologies Office through its work in hydrogen safety, codes, and standards. The Panel’s initiatives in reviewing safety plans, conducting safety evaluations, identifying safety-related technical data gaps, and supporting safety knowledge tools and databases cover the gamut from research and development to demonstration and deployment. The Panel’s recent work has focused on the safe deployment of hydrogen and fuel cell systems in support of DOE efforts to accelerate fuel cell commercialization in early market applications: vehicle refueling, material handling equipment, backup power for warehouses and telecommunication sites, and portable power devices. This paper resulted from observations and considerations stemming from the Panel’s work on early market applications. This paper focuses on hydrogen system components that are installed in outdoor enclosures. These enclosures might alternatively be called “cabinets,” but for simplicity, they are all referred to as “enclosures” in this paper. These enclosures can provide a space where a flammable mixture of hydrogen and air might accumulate, creating the potential for a fire or explosion should an ignition occur. If the enclosure is large enough for a person to enter, and ventilation is inadequate, the hydrogen concentration could be high enough to asphyxiate a person who entered the space. Manufacturers, users, and government authorities rely on requirements described in codes to guide safe design and installation of such systems. Except for small enclosures used for hydrogen gas cylinders (gas cabinets), fuel cell power systems, and the enclosures that most people would describe as buildings, there are no hydrogen safety requirements for these enclosures, leaving gaps that must be addressed. This paper proposes that

  5. Prion propagation in cells expressing PrP glycosylation mutants.

    Science.gov (United States)

    Salamat, Muhammad K; Dron, Michel; Chapuis, Jérôme; Langevin, Christelle; Laude, Hubert

    2011-04-01

    Infection by prions involves conversion of a host-encoded cell surface protein (PrP(C)) to a disease-related isoform (PrP(Sc)). PrP(C) carries two glycosylation sites variably occupied by complex N-glycans, which have been suggested by previous studies to influence the susceptibility to these diseases and to determine characteristics of prion strains. We used the Rov cell system, which is susceptible to sheep prions, to generate a series of PrP(C) glycosylation mutants with mutations at one or both attachment sites. We examined their subcellular trafficking and ability to convert into PrP(Sc) and to sustain stable prion propagation in the absence of wild-type PrP. The susceptibility to infection of mutants monoglycosylated at either site differed dramatically depending on the amino acid substitution. Aglycosylated double mutants showed overaccumulation in the Golgi compartment and failed to be infected. Introduction of an ectopic glycosylation site near the N terminus fully restored cell surface expression of PrP but not convertibility into PrP(Sc), while PrP(C) with three glycosylation sites conferred cell permissiveness to infection similarly to the wild type. In contrast, predominantly aglycosylated molecules with nonmutated N-glycosylation sequons, produced in cells expressing glycosylphosphatidylinositol-anchorless PrP(C), were able to form infectious PrP(Sc). Together our findings suggest that glycosylation is important for efficient trafficking of anchored PrP to the cell surface and sustained prion propagation. However, properly trafficked glycosylation mutants were not necessarily prone to conversion, thus making it difficult in such studies to discern whether the amino acid changes or glycan chain removal most influences the permissiveness to prion infection.

  6. Global Particle-in-Cell Simulations of Mercury's Magnetosphere

    Science.gov (United States)

    Schriver, D.; Travnicek, P. M.; Lapenta, G.; Amaya, J.; Gonzalez, D.; Richard, R. L.; Berchem, J.; Hellinger, P.

    2017-12-01

    Spacecraft observations of Mercury's magnetosphere have shown that kinetic ion and electron particle effects play a major role in the transport, acceleration, and loss of plasma within the magnetospheric system. Kinetic processes include reconnection, the breakdown of particle adiabaticity and wave-particle interactions. Because of the vast range in spatial scales involved in magnetospheric dynamics, from local electron Debye length scales ( meters) to solar wind/planetary magnetic scale lengths (tens to hundreds of planetary radii), fully self-consistent kinetic simulations of a global planetary magnetosphere remain challenging. Most global simulations of Earth's and other planet's magnetosphere are carried out using MHD, enhanced MHD (e.g., Hall MHD), hybrid, or a combination of MHD and particle in cell (PIC) simulations. Here, 3D kinetic self-consistent hybrid (ion particle, electron fluid) and full PIC (ion and electron particle) simulations of the solar wind interaction with Mercury's magnetosphere are carried out. Using the implicit PIC and hybrid simulations, Mercury's relatively small, but highly kinetic magnetosphere will be examined to determine how the self-consistent inclusion of electrons affects magnetic reconnection, particle transport and acceleration of plasma at Mercury. Also the spatial and energy profiles of precipitating magnetospheric ions and electrons onto Mercury's surface, which can strongly affect the regolith in terms of space weathering and particle outflow, will be examined with the PIC and hybrid codes. MESSENGER spacecraft observations are used both to initiate and validate the global kinetic simulations to achieve a deeper understanding of the role kinetic physics play in magnetospheric dynamics.

  7. High Risk Posture on Motor-Manual Short Wood Logging System in Acacia mangium Plantation

    Directory of Open Access Journals (Sweden)

    Efi Yuliati Yovi

    2015-06-01

    Full Text Available Motor-manual logging has been considered as the most dominant logging system in Java Island, Indonesia. The system-which consisted of felling, delimbing, bucking, hauling, and transporting activities- involves a combination of stress factors e.q. difficult work postures, generation of force, and lifting techniques. In the other hand, combination of the three is well associated with high risk of work-related musculoskeletal injuries (MSIs, including musculoskeletal disorders. This research aimed to assess difficult work posture on felling, delimbing, bucking, and manually short wood hauling by employing rapid entire body assessment (REBA technique and muscular pain scoring based on the worker's perceive. It was revealed that felling and manual hauling were scored 4 in the REBA action level, indicated very high MSIs risk level, and categorized as “necessary now” for an injury risk preventive action. The workers' pain scoring indicated that low back (spine in general disorders resulting in low back pain has been considered to be the one of the leading safety issues in the felling and manual hauling. Regardless to complex mechanism of how the personal risk and environmental factors associated with manual material handling injuries, job-related factors approach should be underlined in the MSIs prevention initiative in motor-manual logging. Keywords: motor-manual logging, difficult work posture, REBA, MSIs, low back pain

  8. A system of UAV application in indoor environment

    DEFF Research Database (Denmark)

    Khosiawan, Yohanes; Nielsen, Izabela Ewa

    2016-01-01

    In recent years, there has been an increased demand in the use of multiple unmanned aerial vehicles (UAVs) in indoor environments such as material handling task in a manufacturing environment and plant/environment monitoring task in a greenhouse. However, there is a lack of work reported...

  9. DESIGN AND CONSTRUCTION OF A FORCE-REFLECTING TELEOPERATION SYSTEM

    Energy Technology Data Exchange (ETDEWEB)

    M.A. Ebadian, Ph.D.

    1999-01-01

    For certain applications, such as space servicing, undersea operations, and hazardous material handling tasks in nuclear reactors, the environments can be uncertain, complex, and hazardous. Lives may be in danger if humans were to work under these conditions. As a result, a man-machine system--a teleoperator system--has been developed to work in these types of environments. In a typical teleoperator system, the actual system operates at a remote site; the operator located away from this system usually receives visual information from a video image and/or graphical animation on the computer screen. Additional feedback, such as aural and force information, can significantly enhance performance of the system. Force reflection is a type of feedback in which forces experienced by the remote manipulator are fed back to the manual controller. Various control methods have been proposed for implementation on a teleoperator system. In order to examine different control schemes, a one Degree-Of-Freedom (DOF) Force-Reflecting Manual Controller (FRMC) is constructed and integrated into a PC. The system parameters are identified and constructed as a mathematical model. The Proportional-Integral-Derivative (PID) and fuzzy logic controllers are developed and tested experimentally. Numerical simulation results obtained from the mathematical model are compared with those of experimental data for both types of controllers. In addition, the concept of a telesensation system is introduced. A telesensation system is an advanced teleoperator system that attempts to provide the operator with sensory feedback. In this context, a telesensation system integrates the use of a Virtual Reality (VR) unit, FRMC, and Graphical User Interface (GUI). The VR unit is used to provide the operator with a 3-D visual effect. Various commercial VR units are reviewed and features compared for use in a telesensation system. As for the FRMC, the conceptual design of a 3-DOF FRMC is developed in an effort to

  10. DESIGN AND CONSTRUCTION OF A FORCE-REFLECTING TELEOPERATION SYSTEM

    International Nuclear Information System (INIS)

    Ebadian, M.A.

    1999-01-01

    For certain applications, such as space servicing, undersea operations, and hazardous material handling tasks in nuclear reactors, the environments can be uncertain, complex, and hazardous. Lives may be in danger if humans were to work under these conditions. As a result, a man-machine system--a teleoperator system--has been developed to work in these types of environments. In a typical teleoperator system, the actual system operates at a remote site; the operator located away from this system usually receives visual information from a video image and/or graphical animation on the computer screen. Additional feedback, such as aural and force information, can significantly enhance performance of the system. Force reflection is a type of feedback in which forces experienced by the remote manipulator are fed back to the manual controller. Various control methods have been proposed for implementation on a teleoperator system. In order to examine different control schemes, a one Degree-Of-Freedom (DOF) Force-Reflecting Manual Controller (FRMC) is constructed and integrated into a PC. The system parameters are identified and constructed as a mathematical model. The Proportional-Integral-Derivative (PID) and fuzzy logic controllers are developed and tested experimentally. Numerical simulation results obtained from the mathematical model are compared with those of experimental data for both types of controllers. In addition, the concept of a telesensation system is introduced. A telesensation system is an advanced teleoperator system that attempts to provide the operator with sensory feedback. In this context, a telesensation system integrates the use of a Virtual Reality (VR) unit, FRMC, and Graphical User Interface (GUI). The VR unit is used to provide the operator with a 3-D visual effect. Various commercial VR units are reviewed and features compared for use in a telesensation system. As for the FRMC, the conceptual design of a 3-DOF FRMC is developed in an effort to

  11. A Cost–Effective Computer-Based, Hybrid Motorised and Gravity ...

    African Journals Online (AJOL)

    This has been done with a view to reducing labour costs involved in material handling as well as improving the efficiency of the workflow in the Mauritian apparel industry. Keywords: automated overhead material handling, hybrid system, and productivity. AJOL African Journals Online. HOW TO USE AJOL... for Researchers ...

  12. Proceedings of the Human Factors Society 31st annual meeting

    Energy Technology Data Exchange (ETDEWEB)

    1987-01-01

    Topics covered include: operator/user modeling; automation safety; manual materials handling (includes a paper on materials handling in underground coal mining); impact of human performance on system performance; transportation safety; ergonomic design; mining safety, sleep deprivation and stressors; environmental design; training evaluation and research; and design and evaluation.

  13. Multigrid Particle-in-cell Simulations of Plasma Microturbulence

    Energy Technology Data Exchange (ETDEWEB)

    J.L.V. Lewandowski

    2003-06-17

    A new scheme to accurately retain kinetic electron effects in particle-in-cell (PIC) simulations for the case of electrostatic drift waves is presented. The splitting scheme, which is based on exact separation between adiabatic and on adiabatic electron responses, is shown to yield more accurate linear growth rates than the standard df scheme. The linear and nonlinear elliptic problems that arise in the splitting scheme are solved using a multi-grid solver. The multi-grid particle-in-cell approach offers an attractive path, both from the physics and numerical points of view, to simulate kinetic electron dynamics in global toroidal plasmas.

  14. Fleet servicing facilities for servicing, maintaining, and testing rail and truck radioactive waste transport systems: functional requirements, technical design concepts and options cost estimates and comparisons

    International Nuclear Information System (INIS)

    Watson, C.D.; Hudson, B.J.; Keith, D.A.; Preston, M.K. Jr.; McCreery, P.N.; Knox, W.; Easterling, E.M.; Lamprey, A.S.; Wiedemann, G.

    1980-05-01

    This is a resource document which examines feasibility design concepts and feasibility studies of a Fleet Servicing Facility (FSF). Such a facility is intended to be used for routine servicing, preventive maintenance, and for performing requalification license compliance tests and inspections, minor repairs, and decontamination of both the transportation casks and their associated rail cars or tractor-trailers. None of the United States' waste handling plants presently receiving radioactive wastes have an on-site FSF, nor is there an existing third party facility providing these services. This situation has caused the General Accounting Office to express concern regarding the quality of waste transport system maintenance once the system is placed into service. Thus, a need is indicated for FSF's, or their equivalent, at various radioactive materials receiving sites. In this report, three forms of FSF's solely for spent fuel transport systems were examined: independent, integrated, and colocated. The independent concept was already the subject of a detailed report and is extensively referenced in this document so that capital cost comparisons of the three concepts could be made. These facilities probably could service high-level, intermediate-level, low-level, or other waste transportation systems with minor modification, but this study did not include any system other than spent fuel. Both the Integrated and Colocated concepts were assumed to be associated with some radioactive materials handling facility such as an AFR repository

  15. Milk-run kanban system for raw printed circuit board withdrawal to surface-mounted equipment

    Directory of Open Access Journals (Sweden)

    Swee Li Chee

    2012-12-01

    Full Text Available Purpose:  The paper aims to present a case study and later simulation analysis on a kanban system that incorporating milk-run operation to draw in raw material to the process. Design/methodology/approach:  Data collection at the case study company for ten weeks followed by a process study called value stream mapping. The proposed kanban model is simulated to test its various performances including total output, average flow time, average work-in-process, SME utilization, and average waiting time. Response surface methodology is adopted to generate suitable representative regression models.  Findings: For all performance measures, simulation results showed that the proposed system consistently outperforms the push system currently practiced. Second, the system indicates the advantages of leveling, particularly in the event of machine failure and blockage. Third, operator in the proposed kanban system has a lower utilization, even with the additional material handling task.   Research limitations/implications: This study only begins to reveal the implication of leveling for production control on multi-machine scenario. The simulation of the system is solely based only the case study. The control parameters critical to the case study, were naturally used. The furtherance of the research should include generalizing the system and devising the respective methodology to facilitate wider applications. Practical implications: Originality/value:  The kanban system is proposed in the light of conflicting interests in handling the surface mounting and the related upstream processes. Such aspect is common to electronics assembly industry.

  16. Rapid-mixing studies on the time-scale of radiation damage in cells

    International Nuclear Information System (INIS)

    Adams, G.E.; Michael, B.D.; Asquith, J.C.; Shenoy, M.A.; Watts, M.E.; Whillans, D.W.

    1975-01-01

    Rapid mixing studies were performed to determine the time scale of radiation damage in cells. There is evidence that the sensitizing effects of oxygen and other chemical dose-modifying agents on the response of cells to ionizing radiation involve fast free-radical processes. Fast response technique studies in bacterial systems have shown that extremely fast processes occur when the bacteria are exposed to oxygen or other dose-modifying agents during irradiation. The time scales observed were consistent with the involvement of fast free-radical reactions in the expression of these effects

  17. iPS-Cinderella Story in Cell Biology

    Directory of Open Access Journals (Sweden)

    Editorial

    2010-01-01

    Full Text Available As we step through the frontiers of modern Science, we are all witnesses to the Cinderella story repeating itself in the form of the iPS. The process of re-programming adult somatic cells to derive Induced Pluripotent stem cells (iPS with the wand of transcription factors and then differentiating them back to adult somatic cells resembles the transformation of Cinderella from a Cinder girl to princess and back to a Cinder girl after the ball; but the iPS-Cinderella is the most fascinating thing ever in cell biology!From the day iPS first made its headlines when it was first produced by Shinya Yamanaka at Kyoto University in Japan, Stem Cell scientists all over the world are re- doing their experiments so far done using other sources like embryonic and adult Stem cells with the iPS cells exploring their potential to the fullest. A Stem Cell science news page without this magic word of iPS is difficult to imagine these days and Scientists have been successful in growing most of the adult Cell types from iPS cells.iPS cells was the key to solve the problems of Immune rejection and Immunosupression required when using other allogeneic Stem cell types which had baffled scientists previously. But the issues raised by scientists about the use of viruses and Oncogenes in producing iPS cells were made groundless when scientists in February 2008 published the discovery of a technique that could remove oncogenes after the induction of pluripotency and now it is possible to induce pluripotency using plasmid transfection, piggyback transposon system and piggyback transposon system combined with a non viral vector system. The word of the day is pIPS which are protein-induced Pluripotent stem cells which are iPS cells that were generated without any genetic alteration of the adult cell. This research by the group of Sheng Ding in La Jolla, California made public in April 2009 showed that the generation of poly-arginine anchors was sufficient to induce

  18. A multiresolution remeshed Vortex-In-Cell algorithm using patches

    DEFF Research Database (Denmark)

    Rasmussen, Johannes Tophøj; Cottet, Georges-Henri; Walther, Jens Honore

    2011-01-01

    We present a novel multiresolution Vortex-In-Cell algorithm using patches of varying resolution. The Poisson equation relating the fluid vorticity and velocity is solved using Fast Fourier Transforms subject to free space boundary conditions. Solid boundaries are implemented using the semi...

  19. Recent advances in cell-based therapy for Parkinson disease

    DEFF Research Database (Denmark)

    Astradsson, Arnar; Cooper, Oliver; Vinuela, Angel

    2008-01-01

    In this review, the authors discuss recent advances in the field of cell therapy for Parkinson disease (PD). They compare and contrast recent clinical trials using fetal dopaminergic neurons. They attribute differences in cell preparation techniques, cell type specification, and immunosuppression...

  20. DESIGN OF SMALL AUTOMATION WORK CELL SYSTEM DEMONSTRATIONS

    International Nuclear Information System (INIS)

    TURNER, C.; PEHL, J.

    2000-01-01

    Laboratory (LANL), which are considered to be representative of nuclear material handling glove box operations, the UTRRG has developed three task demonstration concepts to evaluate modular small automation systems. These demonstrations, utilizing 2 to 3 degree-of-freedom systems, include container movement, material transfer via pouring, and container loading. Based on an analysis of the ARIES pilot line specifications, these three simple demonstrations are considered to be similar to approximately 50% of the tasks in the ARIES pilot line. Since these task demonstrations functionally represent basic tasks, they are representative of the current potential of modular small automation technology to a wide spectrum of hazardous applications extending beyond the ARIES pilot line. The selection of these three demonstrations, their design and their effectiveness in demonstrating the potential of modular small automation technology are discussed

  1. REMOTE INTERVENTION TOWER ELIMINATION SYSTEM

    International Nuclear Information System (INIS)

    Dave Murnane; Renauld Washington

    2002-01-01

    This Topical Report is presented to satisfy reporting requirements in the Statement of work section J.5 page 120 per Department of Energy contract DE-AC26-01NT41093. The project does not contain any imperial research data. This report describes the assembly of Commercial off the shelf (COTS) items configured in a unique manner to represent new and innovative technology in the service of size reduction and material handling at DOE sites, to assist in the DandD effort currently underway at the designated DOE Facilities

  2. Role of Geminin in cell fate determination of hematopoietic stem cells (HSCs).

    Science.gov (United States)

    Yasunaga, Shin'ichiro; Ohno, Yoshinori; Shirasu, Naoto; Zhang, Bo; Suzuki-Takedachi, Kyoko; Ohtsubo, Motoaki; Takihara, Yoshihiro

    2016-09-01

    Geminin exerts two distinct molecular roles. Geminin negatively regulates DNA replication licensing through the direct interaction with Cdt1 to prevent re-replication in proliferating cells. Geminin also regulates chromatin remodeling through the direct interaction with Brahma/Brg1 to maintain undifferentiated states of stem cells. We previously uncovered that Polycomb-group complex 1 and Hoxb4/Hoxa9, well-known intrinsic factors that are essential for maintaining the hematopoietic stem cell (HSC) activity, alternatively act as ubiquitin-proteasome systems for Geminin protein to reduce the protein expression level, and sustain the HSC activity. Thus, Geminin is presumed to play an important role in determining cell fate, i.e., turning on and off cellular quiescence and proliferation/differentiation, in HSCs. We recently generated recombinant cell-penetrating Geminin (CP-Geminin), enabling rapid incorporation and withdraw of Geminin protein in cells. CP-Geminin may be useful in regulating the cell cycle and chromatin configuration. In this article, we summarize current information on the molecular functions of Geminin and the regulatory system for Geminin protein expression, and argue for the molecular role of Geminin in cell fate determination of HSCs, and future perspective of a new technology for manipulating the activities of HSCs and cancer stem cells (CSCs).

  3. The replacement of serum by hormones in cell culture media.

    Science.gov (United States)

    Sato, G; Hayashi, I

    1976-12-01

    The replacement of serum by hormones in cell culture media. (Reemplazo del suero por hormonas en el medio de cultivo de células). Arch. Biol. Med. Exper. 10: 120-121, 1976. The serum used in cell culture media can be replaced by a mixture of hormones and some accesory blood factors. The pituitary cell line GH3 can be grown in a medium in which serum is replaced by triiodothyronine, transferrin, parathormone, tyrotrophin releasing hormone and somatomedins. Hela and BHK cell strains can also be grown in serum free medium supplemented with hormones. Each cell type appears to have different hormonal requirements yet it may found that some hormones are required for most cell types.

  4. Raman spectroscopy for DNA quantification in cell nucleus.

    Science.gov (United States)

    Okotrub, K A; Surovtsev, N V; Semeshin, V F; Omelyanchuk, L V

    2015-01-01

    Here we demonstrate the feasibility of a novel approach to quantify DNA in cell nuclei. This approach is based on spectroscopy analysis of Raman light scattering, and avoids the problem of nonstoichiometric binding of dyes to DNA, as it directly measures the signal from DNA. Quantitative analysis of nuclear DNA contribution to Raman spectrum could be reliably performed using intensity of a phosphate mode at 1096 cm(-1) . When compared to the known DNA standards from cells of different animals, our results matched those values at error of 10%. We therefore suggest that this approach will be useful to expand the list of DNA standards, to properly adjust the duration of hydrolysis in Feulgen staining, to assay the applicability of fuchsines for DNA quantification, as well as to measure DNA content in cells with complex hydrolysis patterns, when Feulgen densitometry is inappropriate. © 2014 International Society for Advancement of Cytometry.

  5. Multi-sensors multi-baseline mapping system for mobile robot using stereovision camera and laser-range device

    Directory of Open Access Journals (Sweden)

    Mohammed Faisal

    2016-06-01

    Full Text Available Countless applications today are using mobile robots, including autonomous navigation, security patrolling, housework, search-and-rescue operations, material handling, manufacturing, and automated transportation systems. Regardless of the application, a mobile robot must use a robust autonomous navigation system. Autonomous navigation remains one of the primary challenges in the mobile-robot industry; many control algorithms and techniques have been recently developed that aim to overcome this challenge. Among autonomous navigation methods, vision-based systems have been growing in recent years due to rapid gains in computational power and the reliability of visual sensors. The primary focus of research into vision-based navigation is to allow a mobile robot to navigate in an unstructured environment without collision. In recent years, several researchers have looked at methods for setting up autonomous mobile robots for navigational tasks. Among these methods, stereovision-based navigation is a promising approach for reliable and efficient navigation. In this article, we create and develop a novel mapping system for a robust autonomous navigation system. The main contribution of this article is the fuse of the multi-baseline stereovision (narrow and wide baselines and laser-range reading data to enhance the accuracy of the point cloud, to reduce the ambiguity of correspondence matching, and to extend the field of view of the proposed mapping system to 180°. Another contribution is the pruning the region of interest of the three-dimensional point clouds to reduce the computational burden involved in the stereo process. Therefore, we called the proposed system multi-sensors multi-baseline mapping system. The experimental results illustrate the robustness and accuracy of the proposed system.

  6. Efficient GPU implementation for Particle in Cell algorithm

    Energy Technology Data Exchange (ETDEWEB)

    Joseph, Rejith George [University of Florida; Ravunnikutty, Girish [University of Florida; Ranka, Sanjay [ORNL; Klasky, Scott A [ORNL

    2011-01-01

    Particle in cell method is widely used method in the plasma physics to study the trajectories of charged particles under electromagnetic fields. The PIC algorithm is computationally intensive and its time requirements are proportional to the number of charged particles involved in the simulation. The focus of the paper is to parallelize the PIC algorithm on Graphics Processing Unit (GPU). We present several performance tradeoffs related to the small shared memory and atomic operations on the GPU to achieve high performance.

  7. Structure of the nucleoid in cells of Streptococcus faecalis.

    OpenAIRE

    Daneo-Moore, L; Dicker, D; Higgins, M L

    1980-01-01

    The structure of the nucleoid of Streptococcus faecalis (ATCC 9790) was examined and compared in the unfixed and fixed states by immersive refractometry and electron microscopy. It appears from these studies that the nucleoid structure is much more centralized in unfixed chloramphenicol-treated (stationary-phase) cells than it is in cells in the exponential phase of growth. The more dispersed configuration of the exponential-phase nucleoid could be preserved by fixation in glutaraldehyde, but...

  8. Alternative mitochondrial functions in cell physiopathology: beyond ATP production

    Directory of Open Access Journals (Sweden)

    Kowaltowski A.J.

    2000-01-01

    Full Text Available It is well known that mitochondria are the main site for ATP generation within most tissues. However, mitochondria also participate in a surprising number of alternative activities, including intracellular Ca2+ regulation, thermogenesis and the control of apoptosis. In addition, mitochondria are the main cellular generators of reactive oxygen species, and may trigger necrotic cell death under conditions of oxidative stress. This review concentrates on these alternative mitochondrial functions, and their role in cell physiopathology.

  9. Prototype Manufacturing Technologies: Conversion of Munitions Components into Higher Value Products

    Science.gov (United States)

    2012-05-21

    collection; report generation; dynamic system display; and an interface with the DNC system. Its material handling system is a rail-guided vehicle with...MicroVAX 4000 with tool and fixture management; automatic data collection; report generation; dynamic system display; an interface with the DNC system...generation; dynamic system display; and an interface with the DNC system. Its material handling system is a rail-guided vehicle with one transfer arm and a

  10. β-(1,3)-Glucan Unmasking in Some Candida albicans Mutants Correlates with Increases in Cell Wall Surface Roughness and Decreases in Cell Wall Elasticity.

    Science.gov (United States)

    Hasim, Sahar; Allison, David P; Retterer, Scott T; Hopke, Alex; Wheeler, Robert T; Doktycz, Mitchel J; Reynolds, Todd B

    2017-01-01

    Candida albicans is among the most common human fungal pathogens, causing a broad range of infections, including life-threatening systemic infections. The cell wall of C. albicans is the interface between the fungus and the innate immune system. The cell wall is composed of an outer layer enriched in mannosylated glycoproteins (mannan) and an inner layer enriched in β-(1,3)-glucan and chitin. Detection of C. albicans by Dectin-1, a C-type signaling lectin specific for β-(1,3)-glucan, is important for the innate immune system to recognize systemic fungal infections. Increased exposure of β-(1,3)-glucan to the immune system occurs when the mannan layer is altered or removed in a process called unmasking. Nanoscale changes to the cell wall during unmasking were explored in live cells with atomic force microscopy (AFM). Two mutants, the cho1Δ/Δ and kre5Δ/Δ mutants, were selected as representatives that exhibit modest and strong unmasking, respectively. Comparisons of the cho1Δ/Δ and kre5Δ/Δ mutants to the wild type reveal morphological changes in their cell walls that correlate with decreases in cell wall elasticity. In addition, AFM tips functionalized with Dectin-1 revealed that the forces of binding of Dectin-1 to all of the strains were similar, but the frequency of binding was highest for the kre5Δ/Δ mutant, decreased for the cho1Δ/Δ mutant, and rare for the wild type. These data show that nanoscale changes in surface topology are correlated with increased Dectin-1 adhesion and decreased cell wall elasticity. AFM, using tips functionalized with immunologically relevant molecules, can map epitopes of the cell wall and increase our understanding of pathogen recognition by the immune system. Copyright © 2016 American Society for Microbiology.

  11. Kinetics of Cu (II) separation by ion flotation techniques, in cells with flexible spargers

    International Nuclear Information System (INIS)

    Reyes, M.; Tavera, F. J.; Escudero, R.; Patino, F.; Salinas, E.; Rivera, I.

    2010-01-01

    This research studies and experimentally determines the kinetic parameters and effect of modifying the hydrodynamics and chemical conditions of the air-liquid dispersions during the Cu (II) extraction by ion flotation techniques in cells with porous spargers. Results show that the elimination of Cu (II) from solution can be carried out by ion flotation in one stage, obtaining efficiencies of 68% and 56% for the flat and cylindrical sparger respectively with a xanthate concentration of 0,02 g/l. In multistage systems five cells, recoveries over 92 % were achieved for both sparger geometries. The behavior of the flotation apparent kinetic constant is linear to the parameters that characterize dispersion (Jg, eg y Db), until a point is achieved where the process instability makes the system inoperable. The results show that removing base metal ions by ion flotation is strongly affected by the following factors: collector concentration [C], Jg, eg, Db, Jl and Sb. (Author) 20 refs

  12. Ef: Software for Nonrelativistic Beam Simulation by Particle-in-Cell Algorithm

    Directory of Open Access Journals (Sweden)

    Boytsov A. Yu.

    2018-01-01

    Full Text Available Understanding of particle dynamics is crucial in construction of electron guns, ion sources and other types of nonrelativistic beam devices. Apart from external guiding and focusing systems, a prominent role in evolution of such low-energy beams is played by particle-particle interaction. Numerical simulations taking into account these effects are typically accomplished by a well-known particle-in-cell method. In practice, for convenient work a simulation program should not only implement this method, but also support parallelization, provide integration with CAD systems and allow access to details of the simulation algorithm. To address the formulated requirements, development of a new open source code - Ef - has been started. It's current features and main functionality are presented. Comparison with several analytical models demonstrates good agreement between the numerical results and the theory. Further development plans are discussed.

  13. Ef: Software for Nonrelativistic Beam Simulation by Particle-in-Cell Algorithm

    Science.gov (United States)

    Boytsov, A. Yu.; Bulychev, A. A.

    2018-04-01

    Understanding of particle dynamics is crucial in construction of electron guns, ion sources and other types of nonrelativistic beam devices. Apart from external guiding and focusing systems, a prominent role in evolution of such low-energy beams is played by particle-particle interaction. Numerical simulations taking into account these effects are typically accomplished by a well-known particle-in-cell method. In practice, for convenient work a simulation program should not only implement this method, but also support parallelization, provide integration with CAD systems and allow access to details of the simulation algorithm. To address the formulated requirements, development of a new open source code - Ef - has been started. It's current features and main functionality are presented. Comparison with several analytical models demonstrates good agreement between the numerical results and the theory. Further development plans are discussed.

  14. A thiopurine drug inhibits West Nile virus production in cell culture, but not in mice.

    Science.gov (United States)

    Lim, Pei-Yin; Keating, Julie A; Hoover, Spencer; Striker, Rob; Bernard, Kristen A

    2011-01-01

    Many viruses within the Flavivirus genus cause significant disease in humans; however, effective antivirals against these viruses are not currently available. We have previously shown that a thiopurine drug, 6-methylmercaptopurine riboside (6MMPr), inhibits replication of distantly related viruses within the Flaviviridae family in cell culture, including bovine viral diarrhea virus and hepatitis C virus replicon. Here we further examined the potential antiviral effect of 6MMPr on several diverse flaviviruses. In cell culture, 6MMPr inhibited virus production of yellow fever virus, dengue virus-2 (DENV-2) and West Nile virus (WNV) in a dose-dependent manner, and DENV-2 was significantly more sensitive to 6MMPr treatment than WNV. We then explored the use of 6MMPr as an antiviral against WNV in an immunocompetent mouse model. Once a day treatment of mice with 0.5 mg 6MMPr was just below the toxic dose in our mouse model, and this dose was used in subsequent studies. Mice were treated with 6MMPr immediately after subcutaneous inoculation with WNV for eight consecutive days. Treatment with 6MMPr exacerbated weight loss in WNV-inoculated mice and did not significantly affect mortality. We hypothesized that 6MMPr has low bioavailability in the central nervous system (CNS) and examined the effect of pre-treatment with 6MMPr on viral loads in the periphery and CNS. Pre-treatment with 6MMPr had no significant effect on viremia or viral titers in the periphery, but resulted in significantly higher viral loads in the brain, suggesting that the effect of 6MMPr is tissue-dependent. In conclusion, despite being a potent inhibitor of flaviviruses in cell culture, 6MMPr was not effective against West Nile disease in mice; however, further studies are warranted to reduce the toxicity and/or improve the bioavailability of this potential antiviral drug.

  15. A thiopurine drug inhibits West Nile virus production in cell culture, but not in mice.

    Directory of Open Access Journals (Sweden)

    Pei-Yin Lim

    Full Text Available Many viruses within the Flavivirus genus cause significant disease in humans; however, effective antivirals against these viruses are not currently available. We have previously shown that a thiopurine drug, 6-methylmercaptopurine riboside (6MMPr, inhibits replication of distantly related viruses within the Flaviviridae family in cell culture, including bovine viral diarrhea virus and hepatitis C virus replicon. Here we further examined the potential antiviral effect of 6MMPr on several diverse flaviviruses. In cell culture, 6MMPr inhibited virus production of yellow fever virus, dengue virus-2 (DENV-2 and West Nile virus (WNV in a dose-dependent manner, and DENV-2 was significantly more sensitive to 6MMPr treatment than WNV. We then explored the use of 6MMPr as an antiviral against WNV in an immunocompetent mouse model. Once a day treatment of mice with 0.5 mg 6MMPr was just below the toxic dose in our mouse model, and this dose was used in subsequent studies. Mice were treated with 6MMPr immediately after subcutaneous inoculation with WNV for eight consecutive days. Treatment with 6MMPr exacerbated weight loss in WNV-inoculated mice and did not significantly affect mortality. We hypothesized that 6MMPr has low bioavailability in the central nervous system (CNS and examined the effect of pre-treatment with 6MMPr on viral loads in the periphery and CNS. Pre-treatment with 6MMPr had no significant effect on viremia or viral titers in the periphery, but resulted in significantly higher viral loads in the brain, suggesting that the effect of 6MMPr is tissue-dependent. In conclusion, despite being a potent inhibitor of flaviviruses in cell culture, 6MMPr was not effective against West Nile disease in mice; however, further studies are warranted to reduce the toxicity and/or improve the bioavailability of this potential antiviral drug.

  16. Evidence for P-Glycoprotein Involvement in Cell Volume Regulation Using Coulter Sizing in Flow Cytometry

    Directory of Open Access Journals (Sweden)

    Jennifer Pasquier

    2015-06-01

    Full Text Available The regulation of cell volume is an essential function that is coupled to a variety of physiological processes such as receptor recycling, excitability and contraction, cell proliferation, migration, and programmed cell death. Under stress, cells undergo emergency swelling and respond to such a phenomenon with a regulatory volume decrease (RVD where they release cellular ions, and other osmolytes as well as a concomitant loss of water. The link between P-glycoprotein, a transmembrane transporter, and cell volume regulation is controversial, and changes in cells volume are measured using microscopy or electrophysiology. For instance, by using the patch-clamp method, our team demonstrated that chloride currents activated in the RVD were more intense and rapid in a breast cancer cell line overexpressing the P-glycoprotein (P-gp. The Cell Lab Quanta SC is a flow cytometry system that simultaneously measures electronic volume, side scatter and three fluorescent colors; altogether this provides unsurpassed population resolution and accurate cell counting. Therefore, here we propose a novel method to follow cellular volume. By using the Coulter-type channel of the cytometer Cell Lab Quanta SC MPL (multi-platform loading, we demonstrated a role for the P-gp during different osmotic treatments, but also a differential activity of the P-gp through the cell cycle. Altogether, our data strongly suggests a role of P-gp in cell volume regulation.

  17. Clinical research skills development program in cell-based regenerative medicine.

    Science.gov (United States)

    Schulman, Ivonne Hernandez; Suncion, Viky; Karantalis, Vasileios; Balkan, Wayne; Hare, Joshua M

    2015-02-01

    Cell-based therapy aimed at restoring organ function is one of the most exciting and promising areas of medical research. However, a novel intervention like cell-based therapy requires physician education and training. An increasing number of physicians untrained in regenerative medicine are using cell-based therapy to treat patients for a wide variety of chronic illnesses. The current lack of training for physicians in this area combined with the sharply increasing practice of regenerative medicine is concerning for a number of reasons, namely potential harm to patients and avoidable conflicts between governmental regulatory agencies and physicians. Academic medical fellowship training programs are needed that specifically prepare physicians for treating patients with cell-based therapies for various organ systems and chronic diseases. The National Heart, Lung, and Blood Institute established the Cardiovascular Cell Therapy Network to design and conduct clinical trials that advance the field of cell-based therapy for patients with cardiovascular disease. As part of the network, a two-year Clinical Research Skills Development Program was supported at two centers with the goal of training early career investigators in cell-based clinical and translational research. In this review, we describe the implementation of this training program at our institution with the purpose of promoting the further development of academic fellowship programs in cell-based regenerative medicine. ©AlphaMed Press.

  18. Defined α-synuclein prion-like molecular assemblies spreading in cell culture.

    Science.gov (United States)

    Aulić, Suzana; Le, Tran Thanh Nhat; Moda, Fabio; Abounit, Saïda; Corvaglia, Stefania; Casalis, Loredana; Gustincich, Stefano; Zurzolo, Chiara; Tagliavini, Fabrizio; Legname, Giuseppe

    2014-06-04

    α-Synuclein (α-syn) plays a central role in the pathogenesis of synucleinopathies, a group of neurodegenerative disorders that includes Parkinson disease, dementia with Lewy bodies and multiple system atrophy. Several findings from cell culture and mouse experiments suggest intercellular α-syn transfer. Through a methodology used to obtain synthetic mammalian prions, we tested whether recombinant human α-syn amyloids can promote prion-like accumulation in neuronal cell lines in vitro. A single exposure to amyloid fibrils of human α-syn was sufficient to induce aggregation of endogenous α-syn in human neuroblastoma SH-SY5Y cells. Remarkably, endogenous wild-type α-syn was sufficient for the formation of these aggregates, and overexpression of the protein was not required. Our results provide compelling evidence that endogenous α-syn can accumulate in cell culture after a single exposure to exogenous α-syn short amyloid fibrils. Importantly, using α-syn short amyloid fibrils as seed, endogenous α-syn aggregates and accumulates over several passages in cell culture, providing an excellent tool for potential therapeutic screening of pathogenic α-syn aggregates.

  19. Wavenumber-in-cell simulation of weak Langmuir turbulence

    International Nuclear Information System (INIS)

    Pecseli, H.L.; Trulsen, J.

    1990-01-01

    A wave-number-in-cell code was developed for simulation of a model proposed by Vendenov, Gordeev, and Rudakov for studying weak Langmuir turbulence. The model uses a WKB approach for describing the Langmuir wave field. Theoretical results for damping of ion acoustic waves caused by resonant interaction of Langmuir waves with group velocity around the sound speed were confirmed. Simulations corresponding to an initial condition with an intense wave burst demonstrate a formation of almost stationary localized cavities of intense wideband self-trapped Langmuir wave fields. These cavities have features in common with phase-space vortices

  20. On the osmotically unresponsive water compartment in cells.

    Science.gov (United States)

    Fullerton, Gary D; Kanal, Kalpana M; Cameron, Ivan L

    2006-01-01

    Differences in colligative properties (freezing point, boiling point, vapor pressure and osmotic behavior) between water in living cells and pure bulk water were investigated by re-evaluating reports of the osmotic behavior of mammalian cells. In five different animal cells, osmotically unresponsive water (OUW) values ranged from 1.1 to 2.2 g per g dry mass. Detailed analysis of human red blood cell (RBC) data indicates a major role for hemoglobin OUW-values, aggregation and packing in cell volume regulation that can be explained for the first time in relevant molecular terms.

  1. Particle-in-cell Simulations with Kinetic Electrons

    International Nuclear Information System (INIS)

    Lewandowski, J.L.V.

    2004-01-01

    A new scheme, based on an exact separation between adiabatic and nonadiabatic electron responses, for particle-in-cell (PIC) simulations of drift-type modes is presented. The (linear and nonlinear) elliptic equations for the scalar fields are solved using a multi-grid solver. The new scheme yields linear growth rates in excellent agreement with theory and it is shown to conserve energy well into the nonlinear regime. It is also demonstrated that simulations with few electrons are reliable and accurate, suggesting that large-scale, PIC simulations with electron dynamics in toroidal geometry (e.g., tokamaks and stellarators plasmas) are within reach of present-day massively parallel supercomputers

  2. LANL operating experience with the WAND and HERCULES prototype systems

    International Nuclear Information System (INIS)

    Gruetzmacher, K. M.; Foxx, C. L.; MYERS, S. C.

    2000-01-01

    The Waste Assay for Nonradioactive Disposal (WAND) and the High Efficiency Radiation Counters for Ultimate Low Emission Sensitivity (HERCULES) prototype systems have been operating at Los Alamos National Laboratory's (LANL's) Solid Waste Operation's (SWO'S) non-destructive assay (NDA) building since 1997 and 1998, respectively. These systems are the cornerstone of the verification program for low-density Green is Clean (GIC) waste at the Laboratory. GIC waste includes all non-regulated waste generated in radiological controlled areas (RCAS) that has been actively segregated as clean (i.e., nonradioactive) through the use of waste generator acceptable knowledge (AK). The use of this methodology alters LANL's past practice of disposing of all room trash generated in nuclear facilities in radioactive waste landfills. Waste that is verified clean can be disposed of at the Los Alamos County Landfill. It is estimated that 50-90% of the low-density room trash from radioactive material handling areas at Los Alamos might be free of contamination. This approach avoids the high cost of disposal of clean waste at a radioactive waste landfill. It also reduces consumption of precious space in the radioactive waste landfill where disposal of this waste provides no benefit to the public or the environment. Preserving low level waste (LLW) disposal capacity for truly radioactive waste is critical in this era when expanding existing radioactive waste landfills or permitting new ones is resisted by regulators and stakeholders. This paper describes the operating experience with the WAND and HERCULES since they began operation at SWO. Waste for verification by the WAND system has been limited so far to waste from the Plutonium Facility and the Solid Waste Operations Facility. A total of461 ft3 (13.1 m3) of low-density shredded waste and paper have been verified clean by the WAND system. The HERCULES system has been used to verify waste from four Laboratory facilities. These are the

  3. Testing of serum atherogenicity in cell cultures: questionable data published

    Directory of Open Access Journals (Sweden)

    Sergei V. Jargin

    2012-01-01

    Full Text Available In a large series of studies was reported that culturing of smooth muscle cells with serum from atherosclerosis patients caused intracellular lipid accumulation, while serum from healthy controls had no such effect. Cultures were used for evaluation of antiatherogenic drugs. Numerous substances were reported to lower serum atherogenicity: statins, trapidil, calcium antagonists, garlic derivatives etc. On the contrary, beta-blockers, phenothiazines and oral hypoglycemics were reported to be pro-atherogenic. Known antiatherogenic agents can influence lipid metabolism and cholesterol synthesis, intestinal absorption or endothelium-related mechanisms. All these targets are absent in cell monocultures. Inflammatory factors, addressed by some antiatherogenic drugs, are also not reproduced. In vivo, relationship between cholesterol uptake by cells and atherogenesis must be inverse rather than direct: in familial hypercholesterolemia, inefficient clearance of LDL-cholesterol by cells predisposes to atherosclerosis. Accordingly, if a pharmacological agent reduces cholesterol uptake by cells in vitro, it should be expected to elevate cholesterol in vivo. Validity of clinical recommendations, based on serum atherogenicity testing in cell monocultures, is therefore questionable. These considerations pertain also to the drugs developed on the basis of the cell culture experiments.

  4. Structure of the nucleoid in cells of Streptococcus faecalis.

    Science.gov (United States)

    Daneo-Moore, L; Dicker, D; Higgins, M L

    1980-01-01

    The structure of the nucleoid of Streptococcus faecalis (ATCC 9790) was examined and compared in the unfixed and fixed states by immersive refractometry and electron microscopy. It appears from these studies that the nucleoid structure is much more centralized in unfixed chloramphenicol-treated (stationary-phase) cells than it is in cells in the exponential phase of growth. The more dispersed configuration of the exponential-phase nucleoid could be preserved by fixation in glutaraldehyde, but not in Formalin or in osmium tetroxide. One important factor in explaining these differences in preservation is that glutaraldehyde (but not Formalin or osmium tetroxide) can rapidly cross-link the amino groups of macromolecules in cells. It was also observed that osmium tetroxide resulted in a preferential breakdown of nascent ribonucleic acid. These results are interpreted as indicating that glutaraldehyde is able to stabilize the exponential-phase nucleoid before it assumes the more central appearance seen in osmium tetroxide- and Formalin-fixed cells. These results are discussed in terms of the proposed organization of the exponential-phase nucleoid in unfixed cells. Images PMID:6767695

  5. Scheduling System for Multiple Unmanned Aerial Vehicles in Indoor Environments Using the CSP Approach

    DEFF Research Database (Denmark)

    Park, Youngsoo; Khosiawan, Yohanes; Moon, Ilkyeong

    2016-01-01

    In recent years there has been an increased demand in use of multiple unmanned aerial vehicles (UAVs) for surveillance and material handling tasks in indoor environments. However, only a limited number of studies have been reported on UAV scheduling in an indoor 3D environment. This paper presents...

  6. Particle-in-cell plasma simulations of the modified two-stream instability

    Directory of Open Access Journals (Sweden)

    K. Schlegel

    1994-08-01

    Full Text Available We model the modified two-stream plasma instability occurring in the ionospheric E-region using a 2.5-dimensional particle-in-cell code. Compared to previous similar work we concentrate on simulated quantities that can easily be measured in the real ionosphere by coherent radars or rockets, such as the Doppler velocity, the backscattered power, backscattered spectra, aspect angle behaviour and electron temperature enhancement. Despite using a relatively small simulation model, we obtain remarkably good agreement between actual observed and simulated plasma parameters. The advantage of such a small system is that we were able to perform (other than in previous related work many simulation runs with different sets of input parameters, thus studying the unstable plasma under various conditions.

  7. The use of electromagnetic particle-in-cell codes in accelerator applications

    International Nuclear Information System (INIS)

    Eppley, K.

    1988-12-01

    The techniques developed for the numerical simulation of plasmas have numerous applications relevant to accelerators. The operation of many accelerator components involves transients, interactions between beams and rf fields, and internal plasma oscillations. These effects produce non-linear behavior which can be represented accurately by particle in cell (PIC) simulations. We will give a very brief overview of the algorithms used in PIC Codes. We will examine the range of parameters over which they are useful. We will discuss the factors which determine whether a two or three dimensional simulation is most appropriate. PIC codes have been applied to a wide variety of diverse problems, spanning many of the systems in a linear accelerator. We will present a number of practical examples of the application of these codes to areas such as guns, bunchers, rf sources, beam transport, emittance growth and final focus. 8 refs., 8 figs., 2 tabs

  8. Manufacturing engineering practices in the metal fabrications sector ...

    African Journals Online (AJOL)

    ME involves design and specification of tooling, production processes, process planning and technical problemsolving. ... the conventional type and equipment such as computer numerical control machines (CNCs), industrial robots, automated material handling systems, and flexible manufacturing systems are not in place.

  9. Multimodal Processes Rescheduling

    DEFF Research Database (Denmark)

    Bocewicz, Grzegorz; Banaszak, Zbigniew A.; Nielsen, Peter

    2013-01-01

    Cyclic scheduling problems concerning multimodal processes are usually observed in FMSs producing multi-type parts where the Automated Guided Vehicles System (AGVS) plays a role of a material handling system. Schedulability analysis of concurrently flowing cyclic processes (SCCP) exe...

  10. Clinical significance of metallothioneins in cell therapy and nanomedicine

    Directory of Open Access Journals (Sweden)

    Sharma S

    2013-04-01

    Full Text Available Sushil Sharma,1 Afsha Rais,1 Ranbir Sandhu,1 Wynand Nel,1 Manuchair Ebadi21Saint James School of Medicine, Bonaire, The Netherlands; 2Department of Pharmacology, Physiology, and Therapeutics, Center of Excellence in Neuroscience, University of North Dakota School of Medicine and Health Sciences, Grand Forks, ND, USAAbstract: Mammalian metallothioneins (MTs are low molecular weight (6–7 kDa cysteine-rich proteins that are specifically induced by metal nanoparticles (NPs. MT induction in cell therapy may provide better protection by serving as antioxidant, anti-inflammatory, antiapoptotic agents, and by augmenting zinc-mediated transcriptional regulation of genes involved in cell proliferation and differentiation. Liposome-encapsulated MT-1 promoter has been used extensively to induce growth hormone or other genes in culture and gene-manipulated animals. MTs are induced as a defensive mechanism in chronic inflammatory conditions including neurodegenerative diseases, cardiovascular diseases, cancer, and infections, hence can serve as early and sensitive biomarkers of environmental safety and effectiveness of newly developed NPs for clinical applications. Microarray analysis has indicated that MTs are significantly induced in drug resistant cancers and during radiation treatment. Nutritional stress and environmental toxins (eg, kainic acid and domoic acid induce MTs and aggregation of multilamellar electron-dense membrane stacks (Charnoly body due to mitochondrial degeneration. MTs enhance mitochondrial bioenergetics of reduced nicotinamide adenine dinucleotide–ubiquinone oxidoreductase (complex-1, a rate-limiting enzyme complex involved in the oxidative phosphorylation. Monoamine oxidase-B inhibitors (eg, selegiline inhibit α-synuclein nitration, implicated in Lewy body formation, and inhibit 1-methyl 4-phenylpyridinium and 3-morpholinosydnonimine-induced apoptosis in cultured human dopaminergic neurons and mesencephalic fetal stem cells. MTs

  11. Recent Progress in Cell Reprogramming Technology for Cell Transplantation Therapy.

    Science.gov (United States)

    Yamashita, Toru; Abe, Koji

    2016-01-01

    The discovery of induced pluripotent stem (iPS) cells opened the gate for reprogramming technology with which we can change the cell fate through overexpression of master transcriptional factors. Now we can prepare various kinds of neuronal cells directly induced from somatic cells. It has been reported that overexpression of a neuron-specific transcriptional factors might change the cell fate of endogenous astroglia to neuronal cells in vivo. In addition, some research groups demonstrated that chemical compound can induce chemical-induced neuronal cells, without transcriptional factors overexpression. In this review, we briefly review recent progress in the induced neuronal (iN) cells, and discuss the possibility of application for cell transplantation therapy.

  12. KCNQ1 channels sense small changes in cell volume

    DEFF Research Database (Denmark)

    Grunnet, Morten; Jespersen, Thomas; MacAulay, Nanna

    2003-01-01

    . Incubation of the oocytes in cytochalasin D and experiments with truncated KCNQ1 channels suggest that KCNQ1 channels sense cell volume changes through interactions between the cytoskeleton and the N-terminus of the channel protein. From our results we propose that KCNQ1 and KCNQ4 channels play an important......Many important physiological processes involve changes in cell volume, e.g. the transport of salt and water in epithelial cells and the contraction of cardiomyocytes. In this study, we show that voltage-gated KCNQ1 channels, which are strongly expressed in epithelial cells or cardiomyocytes......, and KCNQ4 channels, expressed in hair cells and the auditory tract, are tightly regulated by small cell volume changes when co-expressed with aquaporin 1 water-channels (AQP1) in Xenopus oocytes. The KCNQ1 and KCNQ4 current amplitudes precisely reflect the volume of the oocytes. By contrast, the related...

  13. The Particle-in-Cell and Kinetic Simulation Software Center

    Science.gov (United States)

    Mori, W. B.; Decyk, V. K.; Tableman, A.; Fonseca, R. A.; Tsung, F. S.; Hu, Q.; Winjum, B. J.; An, W.; Dalichaouch, T. N.; Davidson, A.; Hildebrand, L.; Joglekar, A.; May, J.; Miller, K.; Touati, M.; Xu, X. L.

    2017-10-01

    The UCLA Particle-in-Cell and Kinetic Simulation Software Center (PICKSC) aims to support an international community of PIC and plasma kinetic software developers, users, and educators; to increase the use of this software for accelerating the rate of scientific discovery; and to be a repository of knowledge and history for PIC. We discuss progress towards making available and documenting illustrative open-source software programs and distinct production programs; developing and comparing different PIC algorithms; coordinating the development of resources for the educational use of kinetic software; and the outcomes of our first sponsored OSIRIS users workshop. We also welcome input and discussion from anyone interested in using or developing kinetic software, in obtaining access to our codes, in collaborating, in sharing their own software, or in commenting on how PICKSC can better serve the DPP community. Supported by NSF under Grant ACI-1339893 and by the UCLA Institute for Digital Research and Education.

  14. Wavenumber spectrum of whistler turbulence: Particle-in-cell simulation

    International Nuclear Information System (INIS)

    Saito, S.; Gary, S. Peter; Narita, Y.

    2010-01-01

    The forward cascade of decaying whistler turbulence is studied in low beta plasma to understand essential properties of the energy spectrum at electron scales, by using a two-dimensional electromagnetic particle-in-cell (PIC) simulation. This simulation demonstrates turbulence in which the energy cascade rate is greater than the dissipation rate at the electron inertial length. The PIC simulation shows that the magnetic energy spectrum of forward-cascaded whistler turbulence at electron inertial scales is anisotropic and develops a very steep power-law spectrum which is consistent with recent solar wind observations. A comparison of the simulated spectrum with that predicted by a phenomenological turbulence scaling model suggests that the energy cascade at the electron inertial scale depends on both magnetic fluctuations and electron velocity fluctuations, as well as on the whistler dispersion relation. Thus, not only kinetic Alfven turbulence but also whistler turbulence may explain recent solar wind observations of very steep magnetic spectra at short scales.

  15. Hsp90 inhibitors reduce influenza virus replication in cell culture

    International Nuclear Information System (INIS)

    Chase, Geoffrey; Deng, Tao; Fodor, Ervin; Leung, B.W.; Mayer, Daniel; Schwemmle, Martin; Brownlee, George

    2008-01-01

    The viral RNA polymerase complex of influenza A virus consists of three subunits PB1, PB2 and PA. Recently, the cellular chaperone Hsp90 was shown to play a role in nuclear import and assembly of the trimeric polymerase complex by binding to PB1 and PB2. Here we show that Hsp90 inhibitors, geldanamycin or its derivative 17-AAG, delay the growth of influenza virus in cell culture resulting in a 1-2 log reduction in viral titre early in infection. We suggest that this is caused by the reduced half-life of PB1 and PB2 and inhibition of nuclear import of PB1 and PA which lead to reduction in viral RNP assembly. Hsp90 inhibitors may represent a new class of antiviral compounds against influenza viruses

  16. Form and function in cell motility: from fibroblasts to keratocytes.

    Science.gov (United States)

    Herant, Marc; Dembo, Micah

    2010-04-21

    It is plain enough that a horse is made for running, but similar statements about motile cells are not so obvious. Here the basis for structure-function relations in cell motility is explored by application of a new computational technique that allows realistic three-dimensional simulations of cells migrating on flat substrata. With this approach, some cyber cells spontaneously display the classic irregular protrusion cycles and handmirror morphology of a crawling fibroblast, and others the steady gliding motility and crescent morphology of a fish keratocyte. The keratocyte motif is caused by optimal recycling of the cytoskeleton from the back to the front so that more of the periphery can be devoted to protrusion. These calculations are a step toward bridging the gap between the integrated mechanics and biophysics of whole cells and the microscopic molecular biology of cytoskeletal components. Copyright 2010 Biophysical Society. Published by Elsevier Inc. All rights reserved.

  17. Small molecule alteration of RNA sequence in cells and animals.

    Science.gov (United States)

    Guan, Lirui; Luo, Yiling; Ja, William W; Disney, Matthew D

    2017-10-18

    RNA regulation and maintenance are critical for proper cell function. Small molecules that specifically alter RNA sequence would be exceptionally useful as probes of RNA structure and function or as potential therapeutics. Here, we demonstrate a photochemical approach for altering the trinucleotide expanded repeat causative of myotonic muscular dystrophy type 1 (DM1), r(CUG) exp . The small molecule, 2H-4-Ru, binds to r(CUG) exp and converts guanosine residues to 8-oxo-7,8-dihydroguanosine upon photochemical irradiation. We demonstrate targeted modification upon irradiation in cell culture and in Drosophila larvae provided a diet containing 2H-4-Ru. Our results highlight a general chemical biology approach for altering RNA sequence in vivo by using small molecules and photochemistry. Furthermore, these studies show that addition of 8-oxo-G lesions into RNA 3' untranslated regions does not affect its steady state levels. Copyright © 2017 Elsevier Ltd. All rights reserved.

  18. Propagation and isolation of ranaviruses in cell culture

    DEFF Research Database (Denmark)

    Ariel, Ellen; Nicolajsen, Nicole; Christophersen, Maj-Britt

    2009-01-01

    The optimal in vitro propagation procedure for a panel of ranavirus isolates and the best method for isolation of Epizootic haematopoietic necrosis virus (EHNV) from organ material in cell-culture were investigated. The panel of ranavirus isolates included: Frog virus 3 (FV3), Bohle iridovirus (BIV......), Pike-perch iridovirus (PPIV), European catfish virus (ECV), European sheatfish virus (ESV), EHNV, Doctor fish virus (DFV), Guppy virus 6 (GF6), short-finned eel virus (SERV) and Rana esculenta virus Italy 282/102 (REV 282/102). Each isolate was titrated in five cell lines: bluegill fry (BF-2...... consistently produced lower titers than the other cell lines at all temperatures. The optimal temperature for propagating the isolates collectively to high titers in vivo was 24 °C. Additionally, three established methods for re-isolation of virus from EHNV-infected organ material were compared. Challenged...

  19. Large Deformation Mechanics of Plasma Membrane Chained Vesicles in Cells

    Science.gov (United States)

    Kosawada, Tadashi; Sanada, Kouichi; Takano, Tetsuo

    The clathrin-coated pits, vesicles and chained vesicles on the inner surface of the plasma membrane facilitate the cell to transport specific extracellular macromolecules. This cellular process is strongly involved with large mechanical deformations of the plasma membrane accompanied by changes in membrane curvature. The assembly of the clathrin coat is thought to provide curvature into the membrane. Hence, effects of in-plane shear elasticity due to these coat structure may be significant on the vesicular mechanics. In this study, large deformation mechanics of plasma membrane chained vesicles in cells have been formulated based on minimization of bending and in-plane shear strain energy of the membrane. Effects of outer surrounding cytoplasmic flat membrane upon mechanically stable shapes of the vesicles were revealed, while effects of in-plane shear elasticity were partly discussed.

  20. Direct determination of phosphatase activity from physiological substrates in cells.

    Directory of Open Access Journals (Sweden)

    Zhongyuan Ren

    Full Text Available A direct and continuous approach to determine simultaneously protein and phosphate concentrations in cells and kinetics of phosphate release from physiological substrates by cells without any labeling has been developed. Among the enzymes having a phosphatase activity, tissue non-specific alkaline phosphatase (TNAP performs indispensable, multiple functions in humans. It is expressed in numerous tissues with high levels detected in bones, liver and neurons. It is absolutely required for bone mineralization and also necessary for neurotransmitter synthesis. We provided the proof of concept that infrared spectroscopy is a reliable assay to determine a phosphatase activity in the osteoblasts. For the first time, an overall specific phosphatase activity in cells was determined in a single step by measuring simultaneously protein and substrate concentrations. We found specific activities in osteoblast like cells amounting to 116 ± 13 nmol min(-1 mg(-1 for PPi, to 56 ± 11 nmol min(-1 mg(-1 for AMP, to 79 ± 23 nmol min(-1 mg(-1 for beta-glycerophosphate and to 73 ± 15 nmol min(-1 mg(-1 for 1-alpha-D glucose phosphate. The assay was also effective to monitor phosphatase activity in primary osteoblasts and in matrix vesicles. The use of levamisole--a TNAP inhibitor--served to demonstrate that a part of the phosphatase activity originated from this enzyme. An IC50 value of 1.16 ± 0.03 mM was obtained for the inhibition of phosphatase activity of levamisole in osteoblast like cells. The infrared assay could be extended to determine any type of phosphatase activity in other cells. It may serve as a metabolomic tool to monitor an overall phosphatase activity including acid phosphatases or other related enzymes.

  1. Primary radiation damage and disturbance in cell divisions

    International Nuclear Information System (INIS)

    Kim, Jin Kyu; Lee, Yun-Jong; Kim, Jae-Hun; Petin, Vladislav G.; Nili, Mohammad

    2008-01-01

    Survived cells from a homogeneous population exposed to ionizing radiation form various colonies of different sizes and morphology on a solid nutrient medium, which appear at different time intervals after irradiation. Such a phenomenon agrees well with the modern theory of microdosimetry and classical hit-and-target models of radiobiology. According to the hit-principle, individual cells exposed to the same dose of radiation are damaged in different manners. It means that the survived cells can differ in the content of sublethal damage (hits) produced by the energy absorbed into the cell and which is not enough to give rise to effective radiation damage which is responsible for cell killing or inactivation. In diploid yeast cells, the growth rate of cells from 250 colonies of various sizes appeared at different time intervals after irradiation with 600 Gy of gamma radiation from a 60 Co isotopic source was analyzed. The survival rate after irradiation was 20%. Based on the analyses results, it was possible to categorize the clones grown from irradiated cells according to the number of sub-lesions from 1 to 4. The clones with various numbers of sub-lesions were shown to be different in their viability, radiosensitivity, sensitivity to environmental conditions, and the frequency of recombination and respiratory deficient mutations. Cells from unstable clones exhibited an enhanced radiosensitivity, and an increased portion of morphologically changed cells, nonviable cells and respiration mutants, as well. The degree of expression of the foregoing effects was higher if the number of primary sublethal lesions was greater in the originally irradiated cell. Disturbance in cell division can be characterized by cell inactivation or incorrect distribution of mitochondria between daughter cells. Thus, the suggested methodology of identification of cells with a definite number of primary sublethal lesions will promote further elucidation of the nature of primary radiation

  2. Usulan Tata Letak Ulang Menggunakan Software Quantitative Systems untuk Meminimalkan Jarak Perpindahan Bahan di Lantai Produksi Departemen Mechanic PT Jefta Prakarsa Pratama

    Directory of Open Access Journals (Sweden)

    Khristian Edi Nugroho

    2012-06-01

    Full Text Available The layout applied on the production floor of the Department of Mechanic of PT. Jefta Prakarsa Pratama is not considered as an optimal layout. The reason triggered this optimal level, are some backtrack movements that reduce the effectiveness and efficiency of material handling activities. This research refers to two types of calculation methods. First, manual calculation, which is rectilinear distance measure. Second, computerized calculation, which is Quantitative Systems (QS software. There are three critical criterias referenced in this research, i.e. straight flow of material, more minimal backtrack movement, and more minimal material moving distance. The iteration results using QS software are translated into block layout and then are made as a proposed layout. The rectilinear calculations performed manually are intended to calculate the totalof material moving distance. The data processing obtained a re-layout solution which is more optimal for the production floor of the Department of Mechanic, because it could reduce backtrack distance by 75.28% and the material moving distance by 30.03%. The implementation of the proposed layout is expected to reduce the time of material moving activity on the production floor of the Department of Mechanic, so that the material moving process could run more effectively and efficiently.

  3. Polyunsaturation in cell membranes and lipid bilayers and its effects on membrane proteins.

    Science.gov (United States)

    Slater, S J; Kelly, M B; Yeager, M D; Larkin, J; Ho, C; Stubbs, C D

    1996-03-01

    The effect of variation of the degree of cis-unsaturation on cell membrane protein functioning was investigated using a model lipid bilayer system and protein kinase C (PKC). This protein is a key element of signal transduction. Furthermore it is representative of a class of extrinsic membrane proteins that show lipid dependent interactions with cell membranes. To test for dependence of activity on the phospholipid unsaturation, experiments were devised using a vesicle assay system consisting of phosphatidylcholine (PC) and phosphatidylserine (PS) in which the unsaturation was systematically varied. Highly purified PKC alpha and epsilon were obtained using the baculovirus-insect cell expression system. It was shown that increased PC unsaturation elevated the activity of PKC alpha. By contrast, increasing the unsaturation of PS decreased the activity of PKC alpha, and to a lesser extent PKC epsilon. This result immediately rules out any single lipid bilayer physical parameter, such as lipid order, underlying the effect. It is proposed that while PC unsaturation effects are explainable on the basis of a contribution to membrane surface curvature stress, the effects of PS unsaturation may be due to specific protein-lipid interactions. Overall, the results indicate that altered phospholipid unsaturation in cell membranes that occurs in certain disease states such as chronic alcoholism, or by dietary manipulations, are likely to have profound effects on signal transduction pathways involving PKC and similar proteins.

  4. Targeted inhibition of transcription elongation in cells mediated by triplex-forming oligonucleotides

    Science.gov (United States)

    Faria, M.; Wood, C. D.; Perrouault, L.; Nelson, J. S.; Winter, A.; White, M. R. H.; Hélène, C.; Giovannangeli, C.

    2000-01-01

    Triple-helix-forming oligonucleotides (TFOs) bind in the major groove of double-stranded DNA at oligopyrimidine⋅oligopurine sequences and therefore are candidate molecules for artificial gene regulation, in vitro and in vivo. We recently have described oligonucleotide analogues containing N3′-P5′ phosphoramidate (np) linkages that exhibited efficient inhibition of transcription elongation in vitro. In the present work we provide conclusive evidence that np-modified TFOs targeted to the HIV-1 polypurine tract (PPT) sequence can inhibit transcriptional elongation in cells, either in transient or stable expression systems. The same constructs were used in transient expression assays (target sequence on transfected plasmid) and in the generation of stable cell lines (target sequence integrated into cellular chromosomes). In both cases the only distinguishable feature between the cellular systems is the presence of an insert containing the wild-type PPT/HIV-1 sequence, a mutated version with two mismatches, or the absence of the insert altogether. The inhibitory action induced by np-TFOs was restricted to the cellular systems containing the complementary wild-type PPT/HIV-1 target, and consequently can be attributed only to a triple-helix-mediated mechanism. As a part of this study we also have applied an imaging technique to quantitatively investigate the dynamics of TFO-mediated specific gene silencing in single cells. PMID:10760257

  5. Cholesterol inhibits entotic cell-in-cell formation and actomyosin contraction.

    Science.gov (United States)

    Ruan, Banzhan; Zhang, Bo; Chen, Ang; Yuan, Long; Liang, Jianqing; Wang, Manna; Zhang, Zhengrong; Fan, Jie; Yu, Xiaochen; Zhang, Xin; Niu, Zubiao; Zheng, You; Gu, Songzhi; Liu, Xiaoqing; Du, Hongli; Wang, Jufang; Hu, Xianwen; Gao, Lihua; Chen, Zhaolie; Huang, Hongyan; Wang, Xiaoning; Sun, Qiang

    2018-01-01

    Cell-in-cell structure is prevalent in human cancer, and associated with several specific pathophysiological phenomena. Although cell membrane adhesion molecules were found critical for cell-in-cell formation, the roles of other membrane components, such as lipids, remain to be explored. In this study, we attempted to investigate the effects of cholesterol and phospholipids on the formation of cell-in-cell structures by utilizing liposome as a vector. We found that Lipofectamine-2000, the reagent commonly used for routine transfection, could significantly reduce entotic cell-in-cell formation in a cell-specific manner, which is correlated with suppressed actomyosin contraction as indicated by reduced β-actin expression and myosin light chain phosphorylation. The influence on cell-in-cell formation was likely dictated by specific liposome components as some liposomes affected cell-in-cell formation while some others didn't. Screening on a limited number of lipids, the major components of liposome, identified phosphatidylethanolamine (PE), stearamide (SA), lysophosphatidic acid (LPA) and cholesterol (CHOL) as the inhibitors of cell-in-cell formation. Importantly, cholesterol treatment significantly inhibited myosin light chain phosphorylation, which resembles the effect of Lipofectamine-2000, suggesting cholesterol might be partially responsible for liposomes' effects on cell-in-cell formation. Together, our findings supporting a role of membrane lipids and cholesterol in cell-in-cell formation probably via regulating actomyosin contraction. Copyright © 2017 Elsevier Inc. All rights reserved.

  6. Increasing the fidelity of noncanonical amino acid incorporation in cell-free protein synthesis.

    Science.gov (United States)

    Gan, Qinglei; Fan, Chenguang

    2017-11-01

    Cell-free protein synthesis provides a robust platform for co-translational incorporation of noncanonical amino acid (ncAA) into proteins to facilitate biological studies and biotechnological applications. Recently, eliminating the activity of release factor 1 has been shown to increase ncAA incorporation in response to amber codons. However, this approach could promote mis-incorporation of canonical amino acids by near cognate suppression. We performed a facile protocol to remove near cognate tRNA isoacceptors of the amber codon from total tRNAs, and used the phosphoserine (Sep) incorporation system as validation. By manipulating codon usage of target genes and tRNA species introduced into the cell-free protein synthesis system, we increased the fidelity of Sep incorporation at a specific position. By removing three near cognate tRNA isoacceptors of the amber stop codon [tRNA Lys , tRNA Tyr , and tRNA Gln (CUG)] from the total tRNA, the near cognate suppression decreased by 5-fold without impairing normal protein synthesis in the cell-free protein synthesis system. Mass spectrometry analyses indicated that the fidelity of ncAA incorporation was improved. Removal of near cognate tRNA isoacceptors of the amber codon could increase ncAA incorporation fidelity towards the amber stop codon in release factor deficiency systems. We provide a general strategy to improve fidelity of ncAA incorporation towards stop, quadruplet and sense codons in cell-free protein synthesis systems. This article is part of a Special Issue entitled "Biochemistry of Synthetic Biology - Recent Developments" Guest Editor: Dr. Ilka Heinemann and Dr. Patrick O'Donoghue. Copyright © 2016 Elsevier B.V. All rights reserved.

  7. Inaccurate DNA synthesis in cell extracts of yeast producing active human DNA polymerase iota.

    Directory of Open Access Journals (Sweden)

    Alena V Makarova

    2011-01-01

    Full Text Available Mammalian Pol ι has an unusual combination of properties: it is stimulated by Mn(2+ ions, can bypass some DNA lesions and misincorporates "G" opposite template "T" more frequently than incorporates the correct "A." We recently proposed a method of detection of Pol ι activity in animal cell extracts, based on primer extension opposite the template T with a high concentration of only two nucleotides, dGTP and dATP (incorporation of "G" versus "A" method of Gening, abbreviated as "misGvA". We provide unambiguous proof of the "misGvA" approach concept and extend the applicability of the method for the studies of variants of Pol ι in the yeast model system with different cation cofactors. We produced human Pol ι in baker's yeast, which do not have a POLI ortholog. The "misGvA" activity is absent in cell extracts containing an empty vector, or producing catalytically dead Pol ι, or Pol ι lacking exon 2, but is robust in the strain producing wild-type Pol ι or its catalytic core, or protein with the active center L62I mutant. The signature pattern of primer extension products resulting from inaccurate DNA synthesis by extracts of cells producing either Pol ι or human Pol η is different. The DNA sequence of the template is critical for the detection of the infidelity of DNA synthesis attributed to DNA Pol ι. The primer/template and composition of the exogenous DNA precursor pool can be adapted to monitor replication fidelity in cell extracts expressing various error-prone Pols or mutator variants of accurate Pols. Finally, we demonstrate that the mutation rates in yeast strains producing human DNA Pols ι and η are not elevated over the control strain, despite highly inaccurate DNA synthesis by their extracts.

  8. Exactly energy conserving semi-implicit particle in cell formulation

    Energy Technology Data Exchange (ETDEWEB)

    Lapenta, Giovanni, E-mail: giovanni.lapenta@kuleuven.be

    2017-04-01

    We report a new particle in cell (PIC) method based on the semi-implicit approach. The novelty of the new method is that unlike any of its semi-implicit predecessors at the same time it retains the explicit computational cycle and conserves energy exactly. Recent research has presented fully implicit methods where energy conservation is obtained as part of a non-linear iteration procedure. The new method (referred to as Energy Conserving Semi-Implicit Method, ECSIM), instead, does not require any non-linear iteration and its computational cycle is similar to that of explicit PIC. The properties of the new method are: i) it conserves energy exactly to round-off for any time step or grid spacing; ii) it is unconditionally stable in time, freeing the user from the need to resolve the electron plasma frequency and allowing the user to select any desired time step; iii) it eliminates the constraint of the finite grid instability, allowing the user to select any desired resolution without being forced to resolve the Debye length; iv) the particle mover has a computational complexity identical to that of the explicit PIC, only the field solver has an increased computational cost. The new ECSIM is tested in a number of benchmarks where accuracy and computational performance are tested. - Highlights: • We present a new fully energy conserving semi-implicit particle in cell (PIC) method based on the implicit moment method (IMM). The new method is called Energy Conserving Implicit Moment Method (ECIMM). • The novelty of the new method is that unlike any of its predecessors at the same time it retains the explicit computational cycle and conserves energy exactly. • The new method is unconditionally stable in time, freeing the user from the need to resolve the electron plasma frequency. • The new method eliminates the constraint of the finite grid instability, allowing the user to select any desired resolution without being forced to resolve the Debye length. • These

  9. Slurm: An innovative Particle-in-Cell Method for Magnetohydrodynamics

    Science.gov (United States)

    Bacchini, Fabio; Olshevsky, Vyacheslav; Lapenta, Giovanni

    2016-10-01

    We present a new Particle-in-Cell method for plasma simulations. This is based on the original algorithm of FLIP-MHD, which uses a Lagrangian formulation of the macroscopic equations. A finite-difference approximation of the equations of motion is solved on a fixed (non-moving) grid, while convection of the quantities is modelled with the support of Lagrangian particles. Interpolation with first-order b-splines is used to project the conserved quantities from particles to the grid and back. In this work, we introduce two modifications of the original scheme. A particle volume evolution procedure is adopted to reduce the computational error, based on the Material Point Method for solid mechanics. The additional step introduces little to none computational diffusion and efficiently suppresses the so-called ringing instability, allowing the use of explicit time differencing. Furthermore, we eliminate the need for a Poisson solver in the magnetic field computation with the use of a vector potential. The vector potential evolution is modelled with a moving grid and interpolated to the fixed grid points to obtain a solenoidal magnetic field. The results of a number of HD and MHD tests show good agreement with the reference solutions and rather fast time and space convergence. Air Force Office of Scientific Research, Air Force Materiel Command, USAF under Award No. FA9550-14-1-0375. European Community's Seventh Framework Programme (FP7/2007-2013) via the DEEP-ER project under Grant Agreement No. 610476.

  10. Particle in cell simulation of peaking switch for breakdown evaluation

    Energy Technology Data Exchange (ETDEWEB)

    Umbarkar, Sachin B.; Bindu, S.; Mangalvedekar, H.A.; Saxena, A.; Singh, N.M., E-mail: sachin.b.umbarkar@gmail.com [Department of Electric Engineering, Veermata Jijabai Technological Institute, Mumbai (India); Sharma, Archana; Saroj, P.C.; Mittal, K.C. [Accelerator Pulse Power Division, Bhabha Atomic Research Centre, Mumbai (India)

    2014-07-01

    Marx generator connected to peaking capacitor and peaking switch can generate Ultra-Wideband (UWB) radiation. A new peaking switch is designed for converting the existing nanosecond Marx generator to a UWB source. The paper explains the particle in cell (PIC) simulation for this peaking switch, using MAGIC 3D software. This peaking switch electrode is made up of copper tungsten material and is fixed inside the hermitically sealed derlin material. The switch can withstand a gas pressure up to 13.5 kg/cm{sup 2}. The lower electrode of the switch is connected to the last stage of the Marx generator. Initially Marx generator (without peaking stage) in air; gives the output pulse with peak amplitude of 113.75 kV and pulse rise time of 25 ns. Thus, we design a new peaking switch to improve the rise time of output pulse and to pressurize this peaking switch separately (i.e. Marx and peaking switch is at different pressure). The PIC simulation gives the particle charge density, current density, E counter plot, emitted electron current, and particle energy along the axis of gap between electrodes. The charge injection and electric field dependence on ionic dissociation phenomenon are briefly analyzed using this simulation. The model is simulated with different gases (N{sub 2}, H{sub 2}, and Air) under different pressure (2 kg/cm{sup 2}, 5 kg/cm{sup 2}, 10 kg/cm{sup 2}). (author)

  11. Ultrastructure of Zika virus particles in cell cultures

    Directory of Open Access Journals (Sweden)

    Debora Ferreira Barreto-Vieira

    2016-01-01

    Full Text Available Zika virus (ZIKV has infected thousands of Brazilian people and spread to other American countries since 2015. The introduction of ZIKV brought a strong impact to public health in Brazil. It is of utmost importance to identify a susceptible cell line that will enable the isolation and identification of the virus from patient samples, viral mass production, and testing of drug and vaccine candidates. Besides real-time reverse transcriptase polymerase chain reaction diagnosis for detecting the viral genome, virus isolation in cell lines was useful in order to study the structure of the viral particle and its behaviour inside cells. Analysis of ZIKV infected cell lines was achieved using transmission electron microscopy (TEM. Blood was obtained from a Brazilian patient during the first days after presenting with signs of the disease, and ZIKV from the patient’s blood was isolated in the C6/36 mosquito cell line. Afterwards, Vero cells were inoculated with the viral suspension, fixed six days after inoculation, embedded in polymers, and ultra-thin cut. Like dengue viruses, this flavivirus showed numerous virus particles present inside cellular vesicles thereby confirming the susceptibility of the Vero cell line to ZIKV replication. TEM is a unique technique available to make the virus visible.

  12. Low Voltage Transmission Electron Microscopy in Cell Biology.

    Science.gov (United States)

    Bendayan, Moise; Paransky, Eugene

    2015-07-01

    Low voltage transmission electron microscopy (LVTEM) was employed to examine biological tissues with accelerating voltages as low as 5kV. Tissue preparation was modified to take advantage of the low-voltage techniques. Treatments with heavy metals, such as post-fixation with osmium tetroxide, on block and counterstaining were omitted. Sections (40nm) were thinner than usual and generated highly contrasted images. General appearance of the cells remains similar to that of conventional TEM. New features were however revealed. The matrix of the pancreatic granules displays heterogeneity with partitions that may correspond to the inner-segregation of their secretory proteins. Mitochondria revealed the presence of the ATP synthase granules along their cristea. The nuclear dense chromatin displayed a honeycomb organization while distinct beads, nucleosomes, aligned along thin threads were seen in the dispersed chromatin. Nuclear pore protein complexes revealed their globular nature. The intercalated disks in cardiac muscle displayed their fine structural organization. These features correlate well with data described or predicted by cell and molecular biology. These new aspects are not revealed when thicker and conventionally osmicated tissue sections were examined by LVTEM, indicating that major masking effects are associated with standard TEM techniques. Immunogold was adapted to LVTEM further enhancing its potential in cell biology. Copyright © 2015 Elsevier GmbH. All rights reserved.

  13. Raman hyperspectral imaging of iron transport across membranes in cells

    Science.gov (United States)

    Das, Anupam; Costa, Xavier Felipe; Khmaladze, Alexander; Barroso, Margarida; Sharikova, Anna

    2016-09-01

    Raman scattering microscopy is a powerful imaging technique used to identify chemical composition, structural and conformational state of molecules of complex samples in biology, biophysics, medicine and materials science. In this work, we have shown that Raman techniques allow the measurement of the iron content in protein mixtures and cells. Since the mechanisms of iron acquisition, storage, and excretion by cells are not completely understood, improved knowledge of iron metabolism can offer insight into many diseases in which iron plays a role in the pathogenic process, such as diabetes, neurodegenerative diseases, cancer, and metabolic syndrome. Understanding of the processes involved in cellular iron metabolism will improve our knowledge of cell functioning. It will also have a big impact on treatment of diseases caused by iron deficiency (anemias) and iron overload (hereditary hemochromatosis). Previously, Raman studies have shown substantial differences in spectra of transferrin with and without bound iron, thus proving that it is an appropriate technique to determine the levels of bound iron in the protein mixture. We have extended these studies to obtain hyperspectral images of transferrin in cells. By employing a Raman scanning microscope together with spectral detection by a highly sensitive back-illuminated cooled CCD camera, we were able to rapidly acquire and process images of fixed cells with chemical selectivity. We discuss and compare various methods of hyperspectral Raman image analysis and demonstrate the use of these methods to characterize cellular iron content without the need for dye labeling.

  14. Nanodiamond internalization in cells and the cell uptake mechanism

    International Nuclear Information System (INIS)

    Perevedentseva, E.; Hong, S.-F.; Huang, K.-J.; Chiang, I.-T.; Lee, C.-Y.; Tseng, Y.-T.; Cheng, C.-L.

    2013-01-01

    Cell type-dependent penetration of nanodiamond in living cells is one of the important factors for using nanodiamond as cellular markers/labels, for drug delivery as well as for other biomedical applications. In this work, internalization of 100 nm nanodiamonds by A549 lung human adenocarcinoma cell, Beas-2b non-tumorigenic human bronchial epithelial cell, and HFL-1 fibroblast-like human fetal lung cell is studied and compared. The penetration of nanodiamond into the cells was observed using confocal fluorescence imaging and Raman imaging methods. Visualization of the nanodiamond in cells allows comparison of the internalization for diamond nanoparticles in cancer A549 cell, non-cancer HFL-1, and Beas-2b cells. The dose-dependent and time-dependent behavior of nanodiamond uptake is observed in both cancer as well as non-cancer cells. The mechanism of nanodiamond uptake by cancer and non-cancer cells is analyzed by blocking different pathways. The uptake of nanodiamond in both cancer and non-cancer cells was found predominantly via clathrin-dependent endocytosis. In spite of observed similarity in the uptake mechanism for cancer and non-cancer cells, the nanodiamond uptake for cancer cell quantitatively exceeds the uptake for non-cancer cells, for the studied cell lines. The observed difference in internalization of nanodiamond by cancer and non-cancer cells is discussed

  15. Nanodiamond internalization in cells and the cell uptake mechanism

    Energy Technology Data Exchange (ETDEWEB)

    Perevedentseva, E. [National Dong Hwa University, Department of Physics (China); Hong, S.-F.; Huang, K.-J. [National Dong Hwa University, Department of Life Sciences (China); Chiang, I.-T.; Lee, C.-Y. [National Dong Hwa University, Department of Physics (China); Tseng, Y.-T. [National Dong Hwa University, Department of Life Sciences (China); Cheng, C.-L., E-mail: clcheng@mail.ndhu.edu.tw [National Dong Hwa University, Department of Physics (China)

    2013-08-15

    Cell type-dependent penetration of nanodiamond in living cells is one of the important factors for using nanodiamond as cellular markers/labels, for drug delivery as well as for other biomedical applications. In this work, internalization of 100 nm nanodiamonds by A549 lung human adenocarcinoma cell, Beas-2b non-tumorigenic human bronchial epithelial cell, and HFL-1 fibroblast-like human fetal lung cell is studied and compared. The penetration of nanodiamond into the cells was observed using confocal fluorescence imaging and Raman imaging methods. Visualization of the nanodiamond in cells allows comparison of the internalization for diamond nanoparticles in cancer A549 cell, non-cancer HFL-1, and Beas-2b cells. The dose-dependent and time-dependent behavior of nanodiamond uptake is observed in both cancer as well as non-cancer cells. The mechanism of nanodiamond uptake by cancer and non-cancer cells is analyzed by blocking different pathways. The uptake of nanodiamond in both cancer and non-cancer cells was found predominantly via clathrin-dependent endocytosis. In spite of observed similarity in the uptake mechanism for cancer and non-cancer cells, the nanodiamond uptake for cancer cell quantitatively exceeds the uptake for non-cancer cells, for the studied cell lines. The observed difference in internalization of nanodiamond by cancer and non-cancer cells is discussed.

  16. Membrane tension and cytoskeleton organization in cell motility.

    Science.gov (United States)

    Sens, Pierre; Plastino, Julie

    2015-07-15

    Cell membrane shape changes are important for many aspects of normal biological function, such as tissue development, wound healing and cell division and motility. Various disease states are associated with deregulation of how cells move and change shape, including notably tumor initiation and cancer cell metastasis. Cell motility is powered, in large part, by the controlled assembly and disassembly of the actin cytoskeleton. Much of this dynamic happens in close proximity to the plasma membrane due to the fact that actin assembly factors are membrane-bound, and thus actin filaments are generally oriented such that their growth occurs against or near the membrane. For a long time, the membrane was viewed as a relatively passive scaffold for signaling. However, results from the last five years show that this is not the whole picture, and that the dynamics of the actin cytoskeleton are intimately linked to the mechanics of the cell membrane. In this review, we summarize recent findings concerning the role of plasma membrane mechanics in cell cytoskeleton dynamics and architecture, showing that the cell membrane is not just an envelope or a barrier for actin assembly, but is a master regulator controlling cytoskeleton dynamics and cell polarity.

  17. Author Details

    African Journals Online (AJOL)

    2002) - Articles Scope for the application of an Automated Overhead Material Handling System in the Mauritian Apparel Industry Abstract · Vol 7 (2001) - Articles Microstructural Effect Of Electrochemical Polishing On EDM Machined Surfaces

  18. Guide for training nuclear power plant operators

    International Nuclear Information System (INIS)

    Cox, J.A.; Cagle, C.D.; Corbett, B.L.; Culbert, W.H.; Hamrick, T.P.; Hurt, S.S.; McCord, R.V.; Poteet, K.H.; Bates, A.E.G.; Casto, W.R.

    1977-01-01

    Topics covered include basic preparation, radiation safety and control, principles of reactor operation, general operating characteristics, facility design, safety systems, instrumentation, reactor theory, fuel handling and core parameters, radioactive material handling, and administrative procedures

  19. 75 FR 4536 - Notice of Petitions by Firms for Determination of Eligibility To Apply for Trade Adjustment...

    Science.gov (United States)

    2010-01-28

    ..., whole and frozen fillets. Ketchikan, AK 99901. Materials, salmon, water, ice, knives, packaging; Process..., Bedford furniture. Park, IL 60638. HK Systems 2855 S. James Drive, 1/7/2010 Material handling and logistic...

  20. Guide for training nuclear power plant operators

    Energy Technology Data Exchange (ETDEWEB)

    Cox, J. A.; Cagle, C. D.; Corbett, B. L.; Culbert, W. H.; Hamrick, T. P.; Hurt, S. S.; McCord, R. V.; Poteet, K. H.; Bates, A. E.G.; Casto, W. R.

    1977-01-01

    Topics covered include basic preparation, radiation safety and control, principles of reactor operation, general operating characteristics, facility design, safety systems, instrumentation, reactor theory, fuel handling and core parameters, radioactive material handling, and administrative procedures.

  1. Combat Ration Network for Technology Implementation. Multiple-Unit MRE Leak Detector

    National Research Council Canada - National Science Library

    Litman, Neal

    2000-01-01

    ... testing configuration, materials handling and data collection, and cost/benefits. This STP uses the baseline leak detection system, ATC-3, and determine the impact of such variables as pouch temperature and cartoning...

  2. Multiple-Unit MRE Leak Detector

    National Research Council Canada - National Science Library

    Litman, Neal

    2000-01-01

    ... testing configuration, materials handling and data collection, and cost benefits. This STP uses the baseline leak detection system, ATC-3, and determine the impact of such variables as pouch temperature and cartoning...

  3. Regulatory issues in cell-based therapy for clinical purposes.

    Science.gov (United States)

    Casaroli-Marano, Ricardo P; Tabera, Jaime; Vilarrodona, Anna; Trias, Esteve

    2014-01-01

    Rapid development in the fields of cellular and molecular biology, biotechnology, and bioengineering medicine has brought new, highly innovative treatments and medicinal products, some of which contain viable cells and tissues associated with scaffolds and devices. These new cell-based therapy approaches in regenerative medicine have great potential for use in the treatment of a number of diseases that at present cannot be managed effectively. Given the unique challenges associated with the development of human cell-based medicinal products, great care is required in the development of procedures, practices, and regulation. In cell therapy, appropriate methodologies in the areas of production, reproducibility, maintenance, and delivery are essential for accurate definition and reliable assurance of the suitability and quality of the final products. Recently, the official European Community agencies (EMA) and the relevant authority in the USA (FDA) have made significant efforts to establish regulatory guidance for use in the application of the cell-based therapies for human patients. The guidelines surrounding cell-based therapy take into account the current legislation, but focus less on the heterogeneity and requirements of individual human cell-based products, including specific combination products and applications. When considering guidelines and regulation, a risk assessment approach is an effective method of identifying priority areas for the development of human cell-based medicinal products. Additionally, effective design and thorough validation of the manufacturing process in line with existing Good Manufacturing Practices (GMPs) and quality control regimes and a program that ensures the traceability and biovigilance of the final products are also all essential elements to consider. © 2014 S. Karger AG, Basel.

  4. Particle-in-cell Simulation of Dipolarization Front Associated Whistlers

    Science.gov (United States)

    Lin, D.; Scales, W.; Ganguli, G.; Crabtree, C. E.

    2017-12-01

    Dipolarization fronts (DFs) are dipolarized magnetic field embedded in the Earthward propagating bursty bulk flows (BBFs), which separates the hot, tenuous high-speed flow from the cold, dense, and slowly convecting surrounding plasma [Runov et al. 2011]. Broadband fluctuations have been observed at DFs including the electromagnetic whistler waves and electrostatic lower hybrid waves in the Very Low Frequency (VLF) range [e.g., Zhou et al. 2009, Deng et al. 2010]. There waves are suggested to be able heat electrons and play a critical role in the plasma sheet dynamics [Chaston et al., 2012, Angelopoulos et al., 2013]. However, their generation mechanism and role in the energy conversion are still under debate. The gradient scale of magnetic field, plasma density at DFs in the near-Earth magnetotail is comparable to or lower than the ion gyro radius [Runov et al., 2011, Fu et al., 2012, Breuillard et al., 2016]. Such strongly inhomogeneous configuration could be unstable to the electron-ion hybrid (EIH) instability, which arises from strongly sheared transverse flow and is in the VLF range [Ganguli et al. 1988, Ganguli et al. 2014]. The equilibrium of the EIH theory implies an anisotropy of electron temperature, which are likely to drive the whistler waves observed in DFs [Deng et al., 2010, Gary et al., 2011]. In order to better understand how the whistler waves are generated in DFs and whether the EIH theory is applicable, a fully electromagnetic particle-in-cell (EMPIC) model is used to simulate the EIH instability with similar equilibrium configurations in DF observations. The EMPIC model deals with three dimensions in the velocity space and two dimensions in the configuration space, which is quite ready to include the third configuration dimension. Simulation results will be shown in this presentation.

  5. Novel method for detection of glycogen in cells.

    Science.gov (United States)

    Skurat, Alexander V; Segvich, Dyann M; DePaoli-Roach, Anna A; Roach, Peter J

    2017-05-01

    Glycogen, a branched polymer of glucose, functions as an energy reserve in many living organisms. Abnormalities in glycogen metabolism, usually excessive accumulation, can be caused genetically, most often through mutation of the enzymes directly involved in synthesis and degradation of the polymer leading to a variety of glycogen storage diseases (GSDs). Microscopic visualization of glycogen deposits in cells and tissues is important for the study of normal glycogen metabolism as well as diagnosis of GSDs. Here, we describe a method for the detection of glycogen using a renewable, recombinant protein which contains the carbohydrate-binding module (CBM) from starch-binding domain containing protein 1 (Stbd1). We generated a fusion protein containing g lutathione S-transferase, a cM c eptitope and the tbd1 BM (GYSC) for use as a glycogen-binding probe, which can be detected with secondary antibodies against glutathione S-transferase or cMyc. By enzyme-linked immunosorbent assay, we demonstrate that GYSC binds glycogen and two other polymers of glucose, amylopectin and amylose. Immunofluorescence staining of cultured cells indicate a GYSC-specific signal that is co-localized with signals obtained with anti-glycogen or anti-glycogen synthase antibodies. GYSC-positive staining inside of lysosomes is observed in individual muscle fibers isolated from mice deficient in lysosomal enzyme acid alpha-glucosidase, a well-characterized model of GSD II (Pompe disease). Co-localized GYSC and glycogen signals are also found in muscle fibers isolated from mice deficient in malin, a model for Lafora disease. These data indicate that GYSC is a novel probe that can be used to study glycogen metabolism under normal and pathological conditions. © The Author 2017. Published by Oxford University Press. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com

  6. Polyamines and polyamine biosynthesis in cells exposed to hyperthermia

    Energy Technology Data Exchange (ETDEWEB)

    Gerner, E.W.; Stickney, D.G.; Herman, T.S.; Fuller, D.J.

    1983-02-01

    The issue of how polyamines act to sensitize cultured cells to the lethal effects of hyperthermia was investigated using Chinese hamster cells which were induced to express thermotolerance. Intracellular levels of these naturally occurring polycations were manipulated in certain situations by treating whole cells with methylglyoxal bis-(guanylhydrazone), an inhibitor of the S-adenosyl-L-methionine decarboxylases. Exogenous spermine as low as 100 ..mu..M in the culture media dramatically sensitized cells expressing thermotolerance to the lethal effects of subsequent 42/sup 0/C exposures. When thermotolerance was differentially induced in cultures exposed to 42.4/sup 0/C by varying the rate of heating from 37 to 42.4/sup 0/C, the most resistant cells and the highest levels of intracellular spermidine and spermine. This finding was explainable in part by the observation that the putrescine-dependent S-adenosyl-L-methionine decarboxylase activity was minimally affected in cells expressng the greatest degree of thermotolerance. When this enzyme activity was inhibited by drug, lowered intracellular polyamine levels did not correspond with subsequent survival responses to heat. Interestingly, cultures treated with methylglyoxal bis-(guanylhydrazone) 24 hr previous to heat exposure showed a reduced capacity to express rate of heating-induced thermotolerance. Together, these results demonstrate that the polyamines, especially spermidine and spermine, enhance hyperthermia-induced cell killing by some mechanism involving the plasma membrane. Further, our data suggest that methylglyoxal bis-(guanylhydrazone) can act to affect thermal responses by a mechanism(s) other than modification of intracellular polyamine levels.

  7. Analysis of criticality accident alarm system coverage of the X-744G, X-744H, X-342/344A and X-343 facilities at the Portsmouth Gaseous Diffusion Plant

    Energy Technology Data Exchange (ETDEWEB)

    Dobelbower, M.C.; Woollard, J.; Lee, B.L. Jr.; Tayloe, R.W. Jr.

    1995-09-01

    Additional services for the uranium enrichment cascade process, such as UF{sub 6} feed, sampling, and material storage are provided by several ancillary Uranium Material Handling (UMH) facilities at the PORTS site. These facilities include the X-343 Feed Vaporization and Sampling Facility, the X-744G Bulk Non-Uranium Enrichment Service Activity (UESA) Storage Building, the X-744H Waste Separation and Storage Facility, the X-344A Toll Enrichment Services Facility and the X-342A Feed Vaporization and Fluorine Generation Facility. As uranium operations are performed within these facilities, the potential for a criticality accident exists. In the event of a criticality accident within a process facility at PORTS, a Criticality Accident Alarm System (CAAS) is in place to detect the criticality accident and sound an alarm. In this report, an analysis was performed to provide verification that the existing CAAS at PORTS provides complete criticality accident coverage in the X-343, X-744G. X-744H. X-344A and X-342A facilities. The analysis has determined that all of the above-mentioned facilities have complete CAAS coverage.

  8. LABILE IRON IN CELLS AND BODY FLUIDS . Physiology, Pathology and Pharmacology

    Directory of Open Access Journals (Sweden)

    Zvi Ioav Cabantchik

    2014-03-01

    Full Text Available In living systems iron appears predominantly associated with proteins, but can also be detected in forms referred as labile iron, which denotes the combined redox properties of iron and its amenability to exchange between ligands, including chelators. The labile cell iron (LCI composition varies with metal concentration and substances with chelating groups but also with pH and the redox potential. Although physiologically in the lower µM range, LCI plays a key role in cell iron economy as cross-roads of metabolic pathways. LCI levels are continually regulated by an iron-responsive machinery that balances iron uptake versus deposition into ferritin. However, LCI rises aberrantly in some cell types due to faulty cell utilization pathways or infiltration by pathological iron forms that are found in hemosiderotic plasma. As LCI attains pathological levels, it can catalyze reactive O species (ROS formation that, at particular threshold, can surpass cellular anti-oxidant capacities and seriously damage its constituents. While in normal plasma and interstitial fluids, virtually all iron is securely carried by circulating transferrin (that renders iron essentially non-labile, in systemic iron overload (IO, the total plasma iron binding capacity is often surpassed by a massive iron influx from hyperabsorptive gut or from erythrocyte overburdened spleen and/or liver. As plasma transferrin approaches iron saturation, labile plasma iron (LPI emerges in forms that can infiltrate cells by unregulated routes and raise LCI to toxic levels. Despite the limited knowledge available on LPI speciation in different types and degrees of iron overload, LPI measurements can be and are in fact used for identifying systemic IO and for initiating/adjusting chelation regimens to attain full-day LPI protection. A recent application of labile iron assay is the detection of labile components in iv iron formulations per se as well as in plasma (LPI following parenteral iron

  9. Protective layer formation on magnesium in cell culture medium

    Energy Technology Data Exchange (ETDEWEB)

    Wagener, V.; Virtanen, S., E-mail: virtanen@ww.uni-erlangen.de

    2016-06-01

    In the past, different studies showed that hydroxyapatite (HA) or similar calcium phosphates can be precipitated on Mg during immersion in simulated body fluids. However, at the same time, in most cases a dark grey or black layer is built under the white HA crystals. This layer seems to consist as well of calcium phosphates. Until now, neither the morphology nor its influence on Mg corrosion have been investigated in detail. In this work commercially pure magnesium (cp) was immersed in cell culture medium for one, three and five days at room temperature and in the incubator (37 °C, 5% CO{sub 2}). In addition, the influence of proteins on the formation of a corrosion layer was investigated by adding 20% of fetal calf serum (FCS) to the cell culture medium in the incubator. In order to analyze the formed layers, SEM images of cross sections, X-ray Photoelectron Spectroscopy (XPS), X-ray diffraction (XRD), Energy Dispersive X-ray Spectroscopy (EDX) and Fourier Transformed Infrared Spectroscopy (FTIR) measurements were carried out. Characterization of the corrosion behavior was achieved by electrochemical impedance spectroscopy (EIS) and by potentio-dynamic polarization in Dulbecco's Modified Eagle's Medium (DMEM) at 37 °C. Surface analysis showed that all formed layers consist mainly of amorphous calcium phosphate compounds. For the immersion at room temperature the Ca/P ratio indicates the formation of HA, while in the incubator probably pre-stages to HA are formed. The different immersion conditions lead to a variation in layer thicknesses. However, electrochemical characterization shows that the layer thickness does not influence the corrosion resistance of magnesium. The main influencing factor for the corrosion behavior is the layer morphology. Thus, immersion at room temperature leads to the highest corrosion protection due to the formation of a compact outer layer. Layers formed in the incubator show much worse performances due to completely porous

  10. Development of teleoperated cleanup system

    International Nuclear Information System (INIS)

    Kim, Ki Ho; Park, J. J.; Yang, M. S.; Kwon, H. J.

    2005-01-01

    This report describes the development of a teleoperated cleanup system for use in a highly radioactive environment of DFDF(DUPIC Fuel Demonstration Facility) at KAERI where direct human access to the in-cell is strictly limited. The teleoperated cleanup system was designed to remotely remove contaminants placed or fixed on the floor surface of the hot-cell by mopping them with wet cloth. This cleanup system consists of a mopping slave, a mopping master and a control console. The mopping slave located at the in-cell comprises a mopping tool with a mopping cloth and a mobile platform, which were constructed in modules to facilitate maintenance. The mopping master that is an input device to control the mopping slave has kinematic dissimilarity with the mopping slave. The control console provides a means of bilateral control flows and communications between the mopping master and the mopping slave. In operation, the human operator from the out-of-cell performs a series of decontamination tasks remotely by manipulating the mopping slave located in-cell via a mopping master, having a sense of real mopping. The environmental and mechanical design considerations, and control systems of the developed teleoperated cleanup system are also described

  11. Verification Survey of the Building 4059 Site (Phase B); Post Historical Site Assessment Sites, Block 1; and Radioactive Materials Handling Facility Holdup Pond (Site 4614), Santa Susana Field Laboratory, The Boeing Company, Ventura County, California

    International Nuclear Information System (INIS)

    Vitkus, T.J.

    2008-01-01

    Confirm that the final radiological conditions were accurately and adequately described in the FSS documentation, relative to the established release criteria. The objective of the verification survey was to confirm that the final radiological conditions were accurately and adequately described in the FSS documentation, relative to the established release criteria. This objective was achieved via multiple verification components which included document reviews and independent measurements and sampling. Specifically, documentation of the planning, implementation, and results of the FSS are evaluated; a comparison is performed of the mean residual radionuclide concentrations; site areas are evaluated relative to appropriate FSS classification; and areas are assessed for residual, undocumented contamination

  12. Historical Perspectives and Current Challenges in Cell Microencapsulation

    NARCIS (Netherlands)

    de Vos, Paul; Opara, Emmanuel C.

    2017-01-01

    The principle of immunoisolation of cells is based on encapsulation of cells in immunoprotective but semipermeable membranes that protect cells from hazardous effects of the host immune system but allows ingress of nutrients and outgress of therapeutic molecules. The technology was introduced in

  13. Alterations in cell function with ischemia and shock and their correction.

    Science.gov (United States)

    Chaudry, I H; Clemens, M G; Baue, A E

    1981-10-01

    Progressive cell injury occurs with shock and ischemia, beginning with functional changes in the cell and cell membrane. Membrane transport and potential decrease, Na+ enters and K+ leaves cells; N+-K+ adenosine triphosphatase is activated, adenosine triphosphate (ATP) is used, and mitochondria are stimulated as increased lactate produces acidosis. Energy and cyclic adenosine monophosphate levels decrease, Ca2+ regulation is compromised, and nuclear function and protein synthesis are depressed. The cell swells, and further membrane changes occur with altered hormonal effects and mitochondrial uncoupling. Finally, lysosomes leak, intracellular and mitochondria disruption occurs, and the cell is destroyed. Based on these changes, attempts were made to directly support cell function during low-flow states. After volume replacement and vasoactive agents, other modalities, eg, substrates, membrane-stabilizing solutions, osmotic agents, and energy compounds were used. The use of ATP-MgCl2 was helpful in many experimental low-flow states, with an improvement in cell function mediated by micro-circulatory, cell membrane, or energy-recycling effects. Clinical examples of altered cell and organ function with ischemia and shock are numerous and play a critical role in the development of multiple systems failure. The potential for biochemical support and correction of these problems is now recognized. Benefits have already been achieved in myocardial preservation during cardiac operations, kidney preservation for transplantation, and circulatory and metabolic support of the injured and septic patient.

  14. On the Numerical Dispersion of Electromagnetic Particle-In-Cell Code : Finite Grid Instability

    Energy Technology Data Exchange (ETDEWEB)

    Meyers, Michael David [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Univ. of California, Los Angeles, CA (United States) Dept. of Physics and Astronomy; Huang, Chengkun [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Zeng, Yong [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Yi, Sunghwan [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Albright, Brian James [Los Alamos National Lab. (LANL), Los Alamos, NM (United States)

    2014-07-15

    The Particle-In-Cell (PIC) method is widely used in relativistic particle beam and laser plasma modeling. However, the PIC method exhibits numerical instabilities that can render unphysical simulation results or even destroy the simulation. For electromagnetic relativistic beam and plasma modeling, the most relevant numerical instabilities are the finite grid instability and the numerical Cherenkov instability. We review the numerical dispersion relation of the electromagnetic PIC algorithm to analyze the origin of these instabilities. We rigorously derive the faithful 3D numerical dispersion of the PIC algorithm, and then specialize to the Yee FDTD scheme. In particular, we account for the manner in which the PIC algorithm updates and samples the fields and distribution function. Temporal and spatial phase factors from solving Maxwell's equations on the Yee grid with the leapfrog scheme are also explicitly accounted for. Numerical solutions to the electrostatic-like modes in the 1D dispersion relation for a cold drifting plasma are obtained for parameters of interest. In the succeeding analysis, we investigate how the finite grid instability arises from the interaction of the numerical 1D modes admitted in the system and their aliases. The most significant interaction is due critically to the correct representation of the operators in the dispersion relation. We obtain a simple analytic expression for the peak growth rate due to this interaction.

  15. Characterizing the Accuracy of Higher Order Particle-in-Cell (PIC) Methods

    Science.gov (United States)

    Longley, W.; Fletcher, A.; Dimant, Y. S.; Oppenheim, M. M.

    2016-12-01

    Electrostatic kinetic simulations allow researchers to better understand small-scale processes in the geospace environment. They have been used to understand beam-plasma interactions found in the high latitude magnetosphere-ionosphere system, instabilities and turbulence throughout the ionosphere, as well as a range of other kinetic processes. Advances in numerical methods and computing power have facilitated the development of high-order, massively parallel PIC simulations. We present the first runs from a high-order PIC code, which uses a compact high-order finite difference method with a high-order phase-space volume preserving integrator, and represents macroparticle distributions as cubic splines. We compare the new high-order code with the existing Electrostatic Parallel Particle-in Cell (EPPIC) simulator which uses the Boris mover with traditional tent function macroparticles. We compare the simulators under two sets of initial conditions, using the same grid spacing, time step, and particle parameters in each. First, we simulate a 2D two-stream instability, and look at the growth rate of the instability and the evolution of the resulting electron phase-space holes. Second, we simulate the spectrum from an incoherent scatter radar setup, and look at the signal to noise ratio in each simulation. The high-order PIC is then tuned to run with lower grid resolutions and particle counts until it matches EPPIC's accuracy.

  16. Graphene-augmented nanofiber scaffolds demonstrate new features in cells behaviour

    Science.gov (United States)

    Kazantseva, Jekaterina; Ivanov, Roman; Gasik, Michael; Neuman, Toomas; Hussainova, Irina

    2016-07-01

    Three-dimensional (3D) customized scaffolds capable to mimic a native extracellular matrix open new frontiers in cells manipulation and advanced therapy. The major challenge is in a proper substrate for in vitro models on engineered scaffolds, capable to modulate cells differentiation. Here for the first time we demonstrate novel design and functionality of the 3D porous scaffolds of aligned, self-assembled ceramic nanofibers of ultra-high anisotropy ratio (~107), augmented into graphene shells. This unique hybrid nano-network allows an exceptional combination of selective guidance stimuli of stem cells differentiation, immune reactions variations, and local immobilization of cancer cells, which was not available before. The scaffolds were shown to be able to direct human mesenchymal stem cells (important for stimulation of neuronal and muscle cells) preferential orientation, to suppress major inflammatory factors, and to localize cancer cells; all without additions of specific culture media. The selective downregulation of specific cytokines is anticipated as a new tool for understanding of human immune system and ways of treatment of associated diseases. The effects observed are self-regulated by cells only, without side effects, usually arising from use of external factors. New scaffolds may open new horizons for stem cells fate control such as towards axons and neurites regeneration (Alzheimer’s disease) as well as cancer therapy development.

  17. Conformon-driven biopolymer shape changes in cell modeling.

    Science.gov (United States)

    Ji, Sungchul; Ciobanu, Gabriel

    2003-07-01

    Conceptual models of the atom preceded the mathematical model of the hydrogen atom in physics in the second decade of the 20th century. The computer modeling of the living cell in the 21st century may follow a similar course of development. A conceptual model of the cell called the Bhopalator was formulated in the mid-1980s, along with its twin theories known as the conformon theory of molecular machines and the cell language theory of biopolymer interactions [Ann. N.Y. Acad. Sci. 227 (1974) 211; BioSystems 44 (1997) 17; Ann. N.Y. Acad. Sci. 870 (1999a) 411; BioSystems 54 (2000) 107; Semiotica 138 (1-4) (2002a) 15; Fundamenta Informaticae 49 (2002b) 147]. The conformon theory accounts for the reversible actions of individual biopolymers coupled to irreversible chemical reactions, while the cell language theory provides a theoretical framework for understanding the complex networks of dynamic interactions among biopolymers in the cell. These two theories are reviewed and further elaborated for the benefit of both computational biologists and computer scientists who are interested in modeling the living cell and its functions. One of the critical components of the mechanisms of cell communication and cell computing has been postulated to be space- and time-organized teleonomic (i.e. goal-directed) shape changes of biopolymers that are driven by exergonic (free energy-releasing) chemical reactions. The generalized Franck-Condon principle is suggested to be essential in resolving the apparent paradox arising when one attempts to couple endergonic (free energy-requiring) biopolymer shape changes to the exergonic chemical reactions that are catalyzed by biopolymer shape changes themselves. Conformons, defined as sequence-specific mechanical strains of biopolymers first invoked three decades ago to account for energy coupling in mitochondria, have been identified as shape changers, the agents that cause shape changes in biopolymers. Given a set of space- and time

  18. Historical Perspectives and Current Challenges in Cell Microencapsulation.

    Science.gov (United States)

    de Vos, Paul

    2017-01-01

    The principle of immunoisolation of cells is based on encapsulation of cells in immunoprotective but semipermeable membranes that protect cells from hazardous effects of the host immune system but allows ingress of nutrients and outgress of therapeutic molecules. The technology was introduced in 1933 but has only received its deserved attention for its therapeutic application for three decades now.In the past decade important advances have been made in creating capsules that provoke minimal or no inflammatory responses. There are however new emerging challenges. These challenges relate to optimal nutrition and oxygen supply as well as standardization and documentation of capsule properties.It is concluded that the proof of principle of applicability of encapsulated grafts for treatment of human disease has been demonstrated and merits optimism about its clinical potential. Further innovation requires a much more systematic approach in identifying crucial properties of capsules and cellular grafts to allow sound interpretations of the results.

  19. Fluctuation of Information Entropy Measures in Cell Image

    Directory of Open Access Journals (Sweden)

    Ishay Wohl

    2017-10-01

    Full Text Available A simple, label-free cytometry technique is introduced. It is based on the analysis of the fluctuation of image Gray Level Information Entropy (GLIE which is shown to reflect intracellular biophysical properties like generalized entropy. In this study, the analytical relations between cellular thermodynamic generalized entropy and diffusivity and GLIE fluctuation measures are explored for the first time. The standard deviation (SD of GLIE is shown by experiments, simulation and theoretical analysis to be indifferent to microscope system “noise”. Then, the ability of GLIE fluctuation measures to reflect basic cellular entropy conditions of early death and malignancy is demonstrated in a cell model of human, healthy-donor lymphocytes, malignant Jurkat cells, as well as dead lymphocytes and Jurkat cells. Utilization of GLIE-based fluctuation measures seems to have the advantage of displaying biophysical characterization of the tested cells, like diffusivity and entropy, in a novel, unique, simple and illustrative way.

  20. Imaging in cell-based therapy for neurodegenerative diseases

    International Nuclear Information System (INIS)

    Kirik, Deniz; Breysse, Nathalie; Bjoerklund, Tomas; Besret, Laurent; Hantraye, Philippe

    2005-01-01

    Fetal cell transplantation for the treatment of Parkinson's and Huntington's diseases has been developed over the past two decades and is now in early clinical testing phase. Direct assessment of the graft's survival, integration into the host brain and impact on neuronal functions requires advanced in vivo neuroimaging techniques. Owing to its high sensitivity, positron emission tomography is today the most widely used tool to evaluate the viability and function of the transplanted tissue in the brain. Nuclear magnetic resonance techniques are opening new possibilities for imaging neurochemical events in the brain. The ultimate goal will be to use the combination of multiple imaging modalities for complete functional monitoring of the repair processes in the central nervous system. (orig.)

  1. Synthetic biology in cell-based cancer immunotherapy.

    Science.gov (United States)

    Chakravarti, Deboki; Wong, Wilson W

    2015-08-01

    The adoptive transfer of genetically engineered T cells with cancer-targeting receptors has shown tremendous promise for eradicating tumors in clinical trials. This form of cellular immunotherapy presents a unique opportunity to incorporate advanced systems and synthetic biology approaches to create cancer therapeutics with novel functions. We first review the development of synthetic receptors, switches, and circuits to control the location, duration, and strength of T cell activity against tumors. In addition, we discuss the cellular engineering and genome editing of host cells (or the chassis) to improve the efficacy of cell-based cancer therapeutics, and to reduce the time and cost of manufacturing. Copyright © 2015 Elsevier Ltd. All rights reserved.

  2. System Budgets

    DEFF Research Database (Denmark)

    Jeppesen, Palle

    1996-01-01

    The lecture note is aimed at introducing system budgets for optical communication systems. It treats optical fiber communication systems (six generations), system design, bandwidth effects, other system impairments and optical amplifiers.......The lecture note is aimed at introducing system budgets for optical communication systems. It treats optical fiber communication systems (six generations), system design, bandwidth effects, other system impairments and optical amplifiers....

  3. Hierarchical feedback modules and reaction hubs in cell signaling networks.

    Science.gov (United States)

    Xu, Jianfeng; Lan, Yueheng

    2015-01-01

    Despite much effort, identification of modular structures and study of their organizing and functional roles remain a formidable challenge in molecular systems biology, which, however, is essential in reaching a systematic understanding of large-scale cell regulation networks and hence gaining capacity of exerting effective interference to cell activity. Combining graph theoretic methods with available dynamics information, we successfully retrieved multiple feedback modules of three important signaling networks. These feedbacks are structurally arranged in a hierarchical way and dynamically produce layered temporal profiles of output signals. We found that global and local feedbacks act in very different ways and on distinct features of the information flow conveyed by signal transduction but work highly coordinately to implement specific biological functions. The redundancy embodied with multiple signal-relaying channels and feedback controls bestow great robustness and the reaction hubs seated at junctions of different paths announce their paramount importance through exquisite parameter management. The current investigation reveals intriguing general features of the organization of cell signaling networks and their relevance to biological function, which may find interesting applications in analysis, design and control of bio-networks.

  4. Hierarchical feedback modules and reaction hubs in cell signaling networks.

    Directory of Open Access Journals (Sweden)

    Jianfeng Xu

    Full Text Available Despite much effort, identification of modular structures and study of their organizing and functional roles remain a formidable challenge in molecular systems biology, which, however, is essential in reaching a systematic understanding of large-scale cell regulation networks and hence gaining capacity of exerting effective interference to cell activity. Combining graph theoretic methods with available dynamics information, we successfully retrieved multiple feedback modules of three important signaling networks. These feedbacks are structurally arranged in a hierarchical way and dynamically produce layered temporal profiles of output signals. We found that global and local feedbacks act in very different ways and on distinct features of the information flow conveyed by signal transduction but work highly coordinately to implement specific biological functions. The redundancy embodied with multiple signal-relaying channels and feedback controls bestow great robustness and the reaction hubs seated at junctions of different paths announce their paramount importance through exquisite parameter management. The current investigation reveals intriguing general features of the organization of cell signaling networks and their relevance to biological function, which may find interesting applications in analysis, design and control of bio-networks.

  5. Hierarchical Feedback Modules and Reaction Hubs in Cell Signaling Networks

    Science.gov (United States)

    Xu, Jianfeng; Lan, Yueheng

    2015-01-01

    Despite much effort, identification of modular structures and study of their organizing and functional roles remain a formidable challenge in molecular systems biology, which, however, is essential in reaching a systematic understanding of large-scale cell regulation networks and hence gaining capacity of exerting effective interference to cell activity. Combining graph theoretic methods with available dynamics information, we successfully retrieved multiple feedback modules of three important signaling networks. These feedbacks are structurally arranged in a hierarchical way and dynamically produce layered temporal profiles of output signals. We found that global and local feedbacks act in very different ways and on distinct features of the information flow conveyed by signal transduction but work highly coordinately to implement specific biological functions. The redundancy embodied with multiple signal-relaying channels and feedback controls bestow great robustness and the reaction hubs seated at junctions of different paths announce their paramount importance through exquisite parameter management. The current investigation reveals intriguing general features of the organization of cell signaling networks and their relevance to biological function, which may find interesting applications in analysis, design and control of bio-networks. PMID:25951347

  6. Kinase Screening in Pichia pastoris Identified Promising Targets Involved in Cell Growth and Alcohol Oxidase 1 Promoter (PAOX1 Regulation.

    Directory of Open Access Journals (Sweden)

    Wei Shen

    Full Text Available As one of the most commonly used eukaryotic recombinant protein expression systems, P. pastoris relies heavily on the AOX1 promoter (PAOX1, which is strongly induced by methanol but strictly repressed by glycerol and glucose. However, the complicated signaling pathways involved in PAOX1 regulation when supplemented with different carbon sources are poorly understood. Here we constructed a kinase deletion library in P. pastoris and identified 27 mutants which showed peculiar phenotypes in cell growth or PAOX1 regulation. We analyzed both annotations and possible functions of these 27 targets, and then focused on the MAP kinase Hog1. In order to locate its potential downstream components, we performed the phosphoproteome analysis on glycerol cultured WT and Δhog1 strains and identified 157 differentially phosphorylated proteins. Our results identified important kinases involved in P. pastoris cell growth and PAOX1 regulation, which could serve as valuable targets for further mechanistic studies.

  7. Proteomics in Cell Culture: From Genomics to Combined ‘Omics for Cell Line Engineering and Bioprocess Development

    DEFF Research Database (Denmark)

    Heffner, Kelley; Kaas, Christian Schrøder; Kumar, Amit

    2015-01-01

    The genetic sequencing of Chinese hamster ovary cells has initiated a systems biology era for biotechnology applications. In addition to genomics, critical omics data sets also include proteomics, transcriptomics and metabolomics. Recently, the use of proteomics in cell lines for recombinant...... protein production has increased significantly because proteomics can track changes in protein levels for different cell lines over time, which can be advantageous for bioprocess development and optimization. Specifically, the identification of proteins that affect cell culture processes can aid efforts...... in media development and cell line engineering to improve growth or productivity, delay the onset of apoptosis, or utilize nutrients efficiently. Mass-spectrometry based and other proteomics methods can provide for the detection of thousands of proteins from cell culture and bioinformatics analysis serves...

  8. Melanopsin resets circadian rhythms in cells by inducing clock gene Period1

    Science.gov (United States)

    Yamashita, Shuhei; Uehara, Tomoe; Matsuo, Minako; Kikuchi, Yo; Numano, Rika

    2014-02-01

    The biochemical, physiological and behavioral processes are under the control of internal clocks with the period of approximately 24 hr, circadian rhythms. The expression of clock gene Period1 (Per1) oscillates autonomously in cells and is induced immediately after a light pulse. Per1 is an indispensable member of the central clock system to maintain the autonomous oscillator and synchronize environmental light cycle. Per1 expression could be detected by Per1∷luc and Per1∷GFP plasmid DNA in which firefly luciferase and Green Fluorescence Protein were rhythmically expressed under the control of the mouse Per1 promoter in order to monitor mammalian circadian rhythms. Membrane protein, MELANOPSIN is activated by blue light in the morning on the retina and lead to signals transduction to induce Per1 expression and to reset the phase of circadian rhythms. In this report Per1 induction was measured by reporter signal assay in Per1∷luc and Per1∷GFP fibroblast cell at the input process of circadian rhythms. To the result all process to reset the rhythms by Melanopsin is completed in single cell like in the retina projected to the central clock in the brain. Moreover, the phase of circadian rhythm in Per1∷luc cells is synchronized by photo-activated Melanopsin, because the definite peak of luciferase activity in one dish was found one day after light illumination. That is an available means that physiological circadian rhythms could be real-time monitor as calculable reporter (bioluminescent and fluorescent) chronological signal in both single and groups of cells.

  9. Prion Propagation in Cells Expressing PrP Glycosylation Mutants ▿

    Science.gov (United States)

    Salamat, Muhammad K.; Dron, Michel; Chapuis, Jérôme; Langevin, Christelle; Laude, Hubert

    2011-01-01

    Infection by prions involves conversion of a host-encoded cell surface protein (PrPC) to a disease-related isoform (PrPSc). PrPC carries two glycosylation sites variably occupied by complex N-glycans, which have been suggested by previous studies to influence the susceptibility to these diseases and to determine characteristics of prion strains. We used the Rov cell system, which is susceptible to sheep prions, to generate a series of PrPC glycosylation mutants with mutations at one or both attachment sites. We examined their subcellular trafficking and ability to convert into PrPSc and to sustain stable prion propagation in the absence of wild-type PrP. The susceptibility to infection of mutants monoglycosylated at either site differed dramatically depending on the amino acid substitution. Aglycosylated double mutants showed overaccumulation in the Golgi compartment and failed to be infected. Introduction of an ectopic glycosylation site near the N terminus fully restored cell surface expression of PrP but not convertibility into PrPSc, while PrPC with three glycosylation sites conferred cell permissiveness to infection similarly to the wild type. In contrast, predominantly aglycosylated molecules with nonmutated N-glycosylation sequons, produced in cells expressing glycosylphosphatidylinositol-anchorless PrPC, were able to form infectious PrPSc. Together our findings suggest that glycosylation is important for efficient trafficking of anchored PrP to the cell surface and sustained prion propagation. However, properly trafficked glycosylation mutants were not necessarily prone to conversion, thus making it difficult in such studies to discern whether the amino acid changes or glycan chain removal most influences the permissiveness to prion infection. PMID:21248032

  10. The thermodynamic efficiency of computations made in cells across the range of life

    Science.gov (United States)

    Kempes, Christopher P.; Wolpert, David; Cohen, Zachary; Pérez-Mercader, Juan

    2017-11-01

    Biological organisms must perform computation as they grow, reproduce and evolve. Moreover, ever since Landauer's bound was proposed, it has been known that all computation has some thermodynamic cost-and that the same computation can be achieved with greater or smaller thermodynamic cost depending on how it is implemented. Accordingly an important issue concerning the evolution of life is assessing the thermodynamic efficiency of the computations performed by organisms. This issue is interesting both from the perspective of how close life has come to maximally efficient computation (presumably under the pressure of natural selection), and from the practical perspective of what efficiencies we might hope that engineered biological computers might achieve, especially in comparison with current computational systems. Here we show that the computational efficiency of translation, defined as free energy expended per amino acid operation, outperforms the best supercomputers by several orders of magnitude, and is only about an order of magnitude worse than the Landauer bound. However, this efficiency depends strongly on the size and architecture of the cell in question. In particular, we show that the useful efficiency of an amino acid operation, defined as the bulk energy per amino acid polymerization, decreases for increasing bacterial size and converges to the polymerization cost of the ribosome. This cost of the largest bacteria does not change in cells as we progress through the major evolutionary shifts to both single- and multicellular eukaryotes. However, the rates of total computation per unit mass are non-monotonic in bacteria with increasing cell size, and also change across different biological architectures, including the shift from unicellular to multicellular eukaryotes. This article is part of the themed issue 'Reconceptualizing the origins of life'.

  11. Role of human pulmonary fibroblast-derived MCP-1 in cell activation and migration in experimental silicosis.

    Science.gov (United States)

    Liu, Xueting; Fang, Shencun; Liu, Haijun; Wang, Xingang; Dai, Xiaoniu; Yin, Qing; Yun, Tianwei; Wang, Wei; Zhang, Yingming; Liao, Hong; Zhang, Wei; Yao, Honghong; Chao, Jie

    2015-10-15

    Silicosis is a systemic disease caused by inhaling silicon dioxide (SiO2). Phagocytosis of SiO2 in the lung initiates an inflammatory cascade that results in fibroblast proliferation and migration and subsequent fibrosis. Clinical evidence indicates that the activation of alveolar macrophages by SiO2 produces rapid and sustained inflammation that is characterized by the generation of monocyte chemotactic protein 1 (MCP-1), which induces fibrosis. Pulmonary fibroblast-derived MCP-1 may play a critical role in fibroblast proliferation and migration. Experiments using primary cultured adult human pulmonary fibroblasts (HPF-a) demonstrated the following results: 1) SiO2 treatment resulted in the rapid and sustained induction of MCP-1 as well as the elevation of the CC chemokine receptor type 2 (CCR2) protein levels; 2) pretreatment of HPF-a with RS-102895, a specific CCR2 inhibitor, abolished the SiO2-induced increase in cell activation and migration in both 2D and 3D culture systems; and 3) RNA interference targeting CCR2 prevented the SiO2-induced increase in cell migration. These data demonstrated that the up-regulation of pulmonary fibroblast-derived MCP-1 is involved in pulmonary fibroblast migration induced by SiO2. CCR2 was also up-regulated in response to SiO2, and this up-regulation facilitated the effect of MCP-1 on fibroblasts. Our study deciphered the link between fibroblast-derived MCP-1 and SiO2-induced cell migration. This finding provides novel insight into the potential of MCP-1 in the development of novel therapeutic strategies for silicosis. Copyright © 2015 Elsevier Inc. All rights reserved.

  12. Silver nanoparticle protein corona composition in cell culture media.

    Science.gov (United States)

    Shannahan, Jonathan H; Lai, Xianyin; Ke, Pu Chun; Podila, Ramakrishna; Brown, Jared M; Witzmann, Frank A

    2013-01-01

    The potential applications of nanomaterials as drug delivery systems and in other products continue to expand. Upon introduction into physiological environments and driven by energetics, nanomaterials readily associate proteins forming a protein corona (PC) on their surface. This PC influences the nanomaterial's surface characteristics and may impact their interaction with cells. To determine the biological impact of nanomaterial exposure as well as nanotherapeutic applications, it is necessary to understand PC formation. Utilizing a label-free mass spectrometry-based proteomics approach, we examined the composition of the PC for a set of four silver nanoparticles (AgNPs) including citrate-stabilized and polyvinlypyrrolidone-stabilized (PVP) colloidal silver (20 or 110 nm diameter). To simulate cell culture conditions, AgNPs were incubated for 1 h in Dulbecco's Modified Eagle Medium supplemented with 10% fetal bovine serum, washed, coronal proteins solubilized, and proteins identified and quantified by label-free LC-MS/MS. To determine which attributes influence PC formation, the AgNPs were characterized in both water and cell culture media with 10% FBS. All AgNPs associated a common subset of 11 proteins including albumin, apolipoproteins, keratins, and other serum proteins. 110 nm citrate- and PVP-stabilized AgNPs were found to bind the greatest number of proteins (79 and 85 respectively) compared to 20 nm citrate- and PVP-stabilized AgNPs (45 and 48 respectively), suggesting a difference in PC formation based on surface curvature. While no relationships were found for other protein parameters (isoelectric point or aliphatic index), the PC on 20 nm AgNPs (PVP and citrate) consisted of more hydrophobic proteins compared to 110 nm AgNPs implying that this class of proteins are more receptive to curvature-induced folding and crowding in exchange for an increased hydration in the aqueous environment. These observations demonstrate the significance of electrostatic

  13. Silver nanoparticle protein corona composition in cell culture media.

    Directory of Open Access Journals (Sweden)

    Jonathan H Shannahan

    Full Text Available The potential applications of nanomaterials as drug delivery systems and in other products continue to expand. Upon introduction into physiological environments and driven by energetics, nanomaterials readily associate proteins forming a protein corona (PC on their surface. This PC influences the nanomaterial's surface characteristics and may impact their interaction with cells. To determine the biological impact of nanomaterial exposure as well as nanotherapeutic applications, it is necessary to understand PC formation. Utilizing a label-free mass spectrometry-based proteomics approach, we examined the composition of the PC for a set of four silver nanoparticles (AgNPs including citrate-stabilized and polyvinlypyrrolidone-stabilized (PVP colloidal silver (20 or 110 nm diameter. To simulate cell culture conditions, AgNPs were incubated for 1 h in Dulbecco's Modified Eagle Medium supplemented with 10% fetal bovine serum, washed, coronal proteins solubilized, and proteins identified and quantified by label-free LC-MS/MS. To determine which attributes influence PC formation, the AgNPs were characterized in both water and cell culture media with 10% FBS. All AgNPs associated a common subset of 11 proteins including albumin, apolipoproteins, keratins, and other serum proteins. 110 nm citrate- and PVP-stabilized AgNPs were found to bind the greatest number of proteins (79 and 85 respectively compared to 20 nm citrate- and PVP-stabilized AgNPs (45 and 48 respectively, suggesting a difference in PC formation based on surface curvature. While no relationships were found for other protein parameters (isoelectric point or aliphatic index, the PC on 20 nm AgNPs (PVP and citrate consisted of more hydrophobic proteins compared to 110 nm AgNPs implying that this class of proteins are more receptive to curvature-induced folding and crowding in exchange for an increased hydration in the aqueous environment. These observations demonstrate the significance of

  14. Final Report - "CO2 Sequestration in Cell Biomass of Chlorobium Thiosulfatophilum"

    Energy Technology Data Exchange (ETDEWEB)

    James L. Gaddy, PhD; Ching-Whan Ko, PhD

    2009-05-04

    . crunogena produces a reasonable return when treating a sour gas stream of 120 million SCFD containing 2.5 percent H2S. In this case, the primary source of revenue is from desulfurization of the gas stream. While the technology has significant application in sequestering carbon dioxide in cell biomass or single cell proten (SCP), perhaps the most immediate application is in desulfurizing LGNG or other gas streams. This biological approach is a viable economical alternative to existing hydrogen sulfide removal technology, and is not sensitive to the presence of hydrocarbons which act as catalyst poisons.

  15. Multiplexed quantitative high content screening reveals that cigarette smoke condensate induces changes in cell structure and function through alterations in cell signaling pathways in human bronchial cells

    International Nuclear Information System (INIS)

    Carter, Charleata A.; Hamm, Jonathan T.

    2009-01-01

    Human bronchial cells are one of the first cell types exposed to environmental toxins. Toxins often activate nuclear factor-κB (NF-κB) and protein kinase C (PKC). We evaluated the hypothesis that cigarette smoke condensate (CSC), the particulate fraction of cigarette smoke, activates PKC-α and NF-κB, and concomitantly disrupts the F-actin cytoskeleton, induces apoptosis and alters cell function in BEAS-2B human bronchial epithelial cells. Compared to controls, exposure of BEAS-2B cells to doses of 30 μg/ml CSC significantly activated PKC-α, while CSC doses above 20 μg/ml CSC significantly activated NF-κB. As NF-κB was activated, cell number decreased. CSC treatment of BEAS-2B cells induced a decrease in cell size and an increase in cell surface extensions including filopodia and lamellipodia. CSC treatment of BEAS-2B cells induced F-actin rearrangement such that stress fibers were no longer prominent at the cell periphery and throughout the cells, but relocalized to perinuclear regions. Concurrently, CSC induced an increase in the focal adhesion protein vinculin at the cell periphery. CSC doses above 30 μg/ml induced a significant increase in apoptosis in BEAS-2B cells evidenced by an increase in activated caspase 3, an increase in mitochondrial mass and a decrease in mitochondrial membrane potential. As caspase 3 increased, cell number decreased. CSC doses above 30 μg/ml also induced significant concurrent changes in cell function including decreased cell spreading and motility. CSC initiates a signaling cascade in human bronchial epithelial cells involving PKC-α, NF-κB and caspase 3, and consequently decreases cell spreading and motility. These CSC-induced alterations in cell structure likely prevent cells from performing their normal function thereby contributing to smoke-induced diseases.

  16. Simultaneous characterization of cellular RNA structure and function with in-cell SHAPE-Seq.

    Science.gov (United States)

    Watters, Kyle E; Abbott, Timothy R; Lucks, Julius B

    2016-01-29

    Many non-coding RNAs form structures that interact with cellular machinery to control gene expression. A central goal of molecular and synthetic biology is to uncover design principles linking RNA structure to function to understand and engineer this relationship. Here we report a simple, high-throughput method called in-cell SHAPE-Seq that combines in-cell probing of RNA structure with a measurement of gene expression to simultaneously characterize RNA structure and function in bacterial cells. We use in-cell SHAPE-Seq to study the structure-function relationship of two RNA mechanisms that regulate translation in Escherichia coli. We find that nucleotides that participate in RNA-RNA interactions are highly accessible when their binding partner is absent and that changes in RNA structure due to RNA-RNA interactions can be quantitatively correlated to changes in gene expression. We also characterize the cellular structures of three endogenously expressed non-coding RNAs: 5S rRNA, RNase P and the btuB riboswitch. Finally, a comparison between in-cell and in vitro folded RNA structures revealed remarkable similarities for synthetic RNAs, but significant differences for RNAs that participate in complex cellular interactions. Thus, in-cell SHAPE-Seq represents an easily approachable tool for biologists and engineers to uncover relationships between sequence, structure and function of RNAs in the cell. © The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

  17. In-cell NMR spectroscopy of proteins inside Xenopus laevis oocytes

    International Nuclear Information System (INIS)

    Sakai, Tomomi; Tochio, Hidehito; Tenno, Takeshi; Ito, Yutaka; Kokubo, Tetsuro; Hiroaki, Hidekazu; Shirakawa, Masahiro

    2006-01-01

    In-cell NMR is an application of solution NMR that enables the investigation of protein conformations inside living cells. We have measured in-cell NMR spectra in oocytes from the African clawed frog Xenopus laevis. 15 N-labeled ubiquitin, its derivatives and calmodulin were injected into Xenopus oocytes and two-dimensional 1 H- 15 N correlation spectra of the proteins were obtained. While the spectrum of wild-type ubiquitin in oocytes had rather fewer cross-peaks compared to its in vitro spectrum, ubiquitin derivatives that are presumably unable to bind to ubiquitin-interacting proteins gave a markedly larger number of cross-peaks. This observation suggests that protein-protein interactions between ubiquitin and ubiquitin-interacting proteins may cause NMR signal broadening, and hence spoil the quality of the in-cell HSQC spectra. In addition, we observed the maturation of ubiquitin precursor derivative in living oocytes using the in-cell NMR technique. This process was partly inhibited by pre-addition of ubiquitin aldehyde, a specific inhibitor for ubiquitin C-terminal hydrolase (UCH). Our work demonstrates the potential usefulness of in-cell NMR with Xenopus oocytes for the investigation of protein conformations and functions under intracellular environmental conditions

  18. A high-order particle-in-cell method for low density plasma flow and the simulation of gyrotron resonator devices

    International Nuclear Information System (INIS)

    Stock, Andreas

    2013-01-01

    Within this thesis a parallelized, transient, three-dimensional, high-order discontinuous Galerkin Particle-in-Cell solver is developed and used to simulate the resonant cavity of a gyrotron. The high-order discontinuous Galerkin approach - a Finite-Element type method - provides a fast and efficient algorithm to numerically solve Maxwell's equations used within this thesis. Besides its outstanding dissipation and dispersion properties, the discontinuous Galerkin approach easily allows for using unstructured grids, as required to simulate complex-shaped engineering devices. The discontinuous Galerkin approach approximates a wavelength with significantly less degrees of freedom compared to other methods, e.g. Finite Difference methods. Furthermore, the parallelization capabilities of the discontinuous Galerkin framework are excellent due to the very local dependencies between the elements. These properties are essential for the efficient numerical treatment of the Vlasov-Maxwell system with the Particle-in-Cell method. This system describes the self-consistent interaction of charged particles and the electromagnetic field. As central application within this thesis gyrotron resonators are simulated with the discontinuous Galerkin Particle-in-Cell method on high-performance-computers. The gyrotron is a high-power millimeter wave source, used for the electron cyclotron resonance heating of magnetically confined fusion plasma, e.g. in the Wendelstein 7-X experimental fusion-reactor. Compared to state-of-the-art simulation tools used for the design of gyrotron resonators the Particle-in-Cell method does not use any significant physically simplifications w.r.t. the modelling of the particle-field-interaction, the geometry and the wave-spectrum. Hence, it is the method of choice for validation of current simulation tools being restricted by these simplifications. So far, the Particle-in-Cell method was restricted to be used for demonstration calculations only, because

  19. Effects of common germ-line genetic variation in cell cycle genes on ovarian cancer survival

    DEFF Research Database (Denmark)

    Song, H.; Hogdall, E.; Ramus, S.J.

    2008-01-01

    PURPOSE: Somatic alterations have been shown to correlate with ovarian cancer prognosis and survival, but less is known about the effects on survival of common inherited genetic variation. Of particular interest are genes involved in cell cycle pathways, which regulate cell division and could...... plausibly influence clinical characteristics of multiple tumors types. EXPERIMENTAL DESIGN: We examined associations between common germ-line genetic variation in 14 genes involved in cell cycle pathway (CCND1, CCND2, CCND3, CCNE1, CDKN1A, CDKN1B, CDKN2A, CDKN2B, CDKN2C, CDKN2D, CDK2, CDK4, CDK6, and RB1....... CONCLUSION: It is unlikely that common variants in cell cycle pathways examined above associated with moderate effect in survival after diagnosis of ovarian cancer. Much larger studies will be needed to exclude common variants with small effects Udgivelsesdato: 2008/2/15...

  20. Video imaging of firefly luciferase activity to identify and monitor herpesvirus infection in cell culture.

    Science.gov (United States)

    Mettenleiter, T C; Gräwe, W

    1996-05-01

    Expression of reporter genes incorporated into complex viral genomes is being used increasingly to monitor virus infection in cell culture and in the organism. One of the most sensitive markers is luciferase from Photinus pyralis which catalyzes a luminescent reaction that can be traced in cell and organ extracts in luminometers. A novel method is described for monitoring Iuciferase activity after infection of cells with recombinant herpesvirus (pseudorabies virus) which carries stably and expresses luciferase using ultra high sensitive photon-counting enhanced video image acquisition. The data show that firefly luciferase activity in virus-infected cells can be monitored without destruction of the cells using video equipment for either macroscopic image acquisition or microscopy. Resolution down to a single-cell level can thereby be achieved. This method increases greatly the potential of monitoring virus infection in real time with a non-destructive highly sensitive method in cell culture and should also help to assay viral spread in the animal.

  1. Reduction of hepatitis C virus NS5A hyperphosphorylation by selective inhibition of cellular kinases activates viral RNA replication in cell culture.

    Science.gov (United States)

    Neddermann, Petra; Quintavalle, Manuela; Di Pietro, Chiara; Clementi, Angelica; Cerretani, Mauro; Altamura, Sergio; Bartholomew, Linda; De Francesco, Raffaele

    2004-12-01

    Efficient replication of hepatitis C virus (HCV) subgenomic RNA in cell culture requires the introduction of adaptive mutations. In this report we describe a system which enables efficient replication of the Con1 subgenomic replicon in Huh7 cells without the introduction of adaptive mutations. The starting hypothesis was that high amounts of the NS5A hyperphosphorylated form, p58, inhibit replication and that reduction of p58 by inhibition of specific kinase(s) below a certain threshold enables HCV replication. Upon screening of a panel of kinase inhibitors, we selected three compounds which inhibited NS5A phosphorylation in vitro and the formation of NS5A p58 in cell culture. Cells, transfected with the HCV Con1 wild-type sequence, support HCV RNA replication upon addition of any of the three compounds. The effect of the kinase inhibitors was found to be synergistic with coadaptive mutations in NS3. This is the first direct demonstration that the presence of high amounts of NS5A-p58 causes inhibition of HCV RNA replication in cell culture and that this inhibition can be relieved by kinase inhibitors.

  2. Ventilation systems

    International Nuclear Information System (INIS)

    Gossler

    1980-01-01

    The present paper deals with - controlled area ventilation systems - ventilation systems for switchgear-building and control-room - other ventilation systems for safety equipments - service systems for ventilation systems. (orig./RW)

  3. Multi-grid Particle-in-cell Simulations of Plasma Microturbulence

    International Nuclear Information System (INIS)

    Lewandowski, J.L.V.

    2003-01-01

    A new scheme to accurately retain kinetic electron effects in particle-in-cell (PIC) simulations for the case of electrostatic drift waves is presented. The splitting scheme, which is based on exact separation between adiabatic and on adiabatic electron responses, is shown to yield more accurate linear growth rates than the standard df scheme. The linear and nonlinear elliptic problems that arise in the splitting scheme are solved using a multi-grid solver. The multi-grid particle-in-cell approach offers an attractive path, both from the physics and numerical points of view, to simulate kinetic electron dynamics in global toroidal plasmas

  4. Assembly of Lyotropic Liquid Crystals with Solid Crystal's Structural Order Translated from the Lipid Rafts in Cell Membranes.

    Science.gov (United States)

    Lee, Yoon Seob

    2017-11-29

    Self-assembly offers a powerful way to control the complexity and hierarchy of nanoscale materials, and promises to create a diverse range of emergent properties. Successful syntheses that allow a delicate structural design of building units play an important role. However, as can be learned from many cellular processes and functions, coself-assembly using logically chosen additives should be equally effective in designing self-assembly. Herein I show that, translated from the dynamic nanoscale assemblies in cell membranes known as lipid rafts, coself-assembly of 1-decanol into cetyltrimethylammonium chloride micelles for the assembly of lyotropic liquid crystals generates new structural complexity and hierarchy, and a surprising property that is emerging from it. Designing the intermolecular forces in the way that cholesterol interacts with sphingolipids promotes the synergistic balance between the flexibility and rigidity, and the unique molecular recognition for silicic acid, followed by the micelle coalescence. This very much resembles the assembly process of the lipid rafts in cell membranes and triggers orders of magnitude of sharp increases in X-ray diffraction intensity. The analysis of the diffraction patterns shows that the structural order of these liquid crystals matches that of solid crystals, often of single crystals. Furthermore, the assembly of the liquid crystals promotes a substantial increase in the condensation rate of silicic acids by guiding them to form a silicate trimer along the surface of micelles. This very much resembles the role of the lipid rafts that sharply increases the reaction rate of biomolecules by guiding them to form discrete species along the surface of membranes. This finding demonstrates that it is possible to translate the key features of cellular processes and functions into artificial self-assembling systems of our choice using the building units that are readily available, thus creating novel soft materials.

  5. Cell growth, intracellular calcium concentration and metabolic cooperation measured in cells exposed to 50 Hz electromagnetic fields

    International Nuclear Information System (INIS)

    Skauli, K.S.

    1996-08-01

    Colony-forming efficiency, DNA/protein and DNA/cell were measured in cells exposed to magnetic fields of 0.2 and 1 mT at a frequency of 50 Hz. Intracellular calcium concentrations were measured in cells exposed to 0.3 and 1 mT at 50 Hz. Metabolic cooperation was measured in cells exposed to 1 mT at 50 Hz. No significant effects of the fields were observed. 20 refs., 10 figs

  6. Dynamic Pedagogy for Effective Training of Youths in Cell Phone Maintenance

    Science.gov (United States)

    Ogbuanya, T. C.; Jimoh, Bakare

    2015-01-01

    The study determined dynamic pedagogies for effective training of youths in cell phone maintenance. The study was conducted in Enugu State of Nigeria. Four research questions were developed while four null hypotheses formulated were tested at 0.05 level of significance. A survey research design was adopted for the study. The population for the…

  7. Development of 2D particle-in-cell code to simulate high current, low ...

    Indian Academy of Sciences (India)

    Abstract. A code for 2D space-charge dominated beam dynamics study in beam trans- port lines is developed. The code is used for particle-in-cell (PIC) simulation of z-uniform beam in a channel containing solenoids and drift space. It can also simulate a transport line where quadrupoles are used for focusing the beam.

  8. A 31-residue peptide induces aggregation of tau's microtubule-binding region in cells

    Science.gov (United States)

    Stöhr, Jan; Wu, Haifan; Nick, Mimi; Wu, Yibing; Bhate, Manasi; Condello, Carlo; Johnson, Noah; Rodgers, Jeffrey; Lemmin, Thomas; Acharya, Srabasti; Becker, Julia; Robinson, Kathleen; Kelly, Mark J. S.; Gai, Feng; Stubbs, Gerald; Prusiner, Stanley B.; Degrado, William F.

    2017-09-01

    The self-propagation of misfolded conformations of tau underlies neurodegenerative diseases, including Alzheimer's. There is considerable interest in discovering the minimal sequence and active conformational nucleus that defines this self-propagating event. The microtubule-binding region, spanning residues 244-372, reproduces much of the aggregation behaviour of tau in cells and animal models. Further dissection of the amyloid-forming region to a hexapeptide from the third microtubule-binding repeat resulted in a peptide that rapidly forms fibrils in vitro. We show that this peptide lacks the ability to seed aggregation of tau244-372 in cells. However, as the hexapeptide is gradually extended to 31 residues, the peptides aggregate more slowly and gain potent activity to induce aggregation of tau244-372 in cells. X-ray fibre diffraction, hydrogen-deuterium exchange and solid-state NMR studies map the beta-forming region to a 25-residue sequence. Thus, the nucleus for self-propagating aggregation of tau244-372 in cells is packaged in a remarkably small peptide.

  9. Identification of miRNA targets with stable isotope labeling by amino acids in cell culture

    DEFF Research Database (Denmark)

    Vinther, Jeppe; Hedegaard, Mads Marquardt; Gardner, Paul Phillip

    2006-01-01

    miRNAs are small noncoding RNAs that regulate gene expression. We have used stable isotope labeling by amino acids in cell culture (SILAC) to investigate the effect of miRNA-1 on the HeLa cell proteome. Expression of 12 out of 504 investigated proteins was repressed by miRNA-1 transfection...

  10. The 2012 Ming K Jeang award for excellence in cell & bioscience.

    Science.gov (United States)

    Shi, Yun-Bo

    2013-05-23

    Three research groups led by Dr. Lixin Feng of Shanghai JiaoTong University School of Medicine, Shanghai, China, Dr. Walter Wahli of University of Lausanne, Lausanne, Switzerland, and Dr. Gutian Xiao of University of Pittsburgh Cancer Institute, Pittsburgh, USA, won the 2012 Ming K Jeang Award for Excellence in Cell & Bioscience.

  11. Load management strategy for Particle-In-Cell simulations in high energy physics

    DEFF Research Database (Denmark)

    Beck, Arnaud; Frederiksen, Jacob Trier; Derouillat, Julien

    2016-01-01

    illustrate some of these limitations in the context of electron laser wakefield acceleration (LWFA). The main limitation we identify in state-of-the-art Particle-In-Cell (PIC) codes is computational load imbalance. We propose an innovative algorithm to deal with this specific issue as well as milestones...

  12. Plume expansion of a laser-induced plasma studied with the particle-in-cell method

    DEFF Research Database (Denmark)

    Ellegaard, Ole; Nedela, T; Urbassek, H

    2002-01-01

     The initial stage of laser-induced plasma plume expansion from a solid in vacuum and the effect of the Coulomb field have been studied. We have performed a one-dimensional numerical calculation by mapping the charge on a computational grid according to the particle-in-cell (PIC) method of Birdsall...

  13. Radiation-induced irreparable heritable changes in cells promoting their tumoral transformation

    International Nuclear Information System (INIS)

    Kuzin, A.M.; Vagabova, M.Eh.; Yurov, S.S.

    1988-01-01

    In experiments with model plant tumors (Kalanchoe-ti plasmid Agrobat. tumefaciens C-58D) it was shown that exposure of the recepient plant to low-level γ-radiation of Gy induced changes in cells that were not repaired over two months promoting tumoral transformations in them. Those changes were shown to persist in the offspring of the exposed somatic cells

  14. Thioridazine affects transcription of genes involved in cell wall biosynthesis in methicillin-resistant Staphylococcus aureus

    DEFF Research Database (Denmark)

    Bonde, Mette; Højland, Dorte Heidi; Kolmos, Hans Jørn

    2011-01-01

    have previously shown that the expression of some resistance genes is abolished after treatment with thioridazine and oxacillin. To further understand the mechanism underlying the reversal of resistance, we tested the expression of genes involved in antibiotic resistance and cell wall biosynthesis...... reversal of resistance by thioridazine relies on decreased expression of specific genes involved in cell wall biosynthesis....

  15. Insights into the function and dysfunction of α-synuclein in cells

    NARCIS (Netherlands)

    Raiss, C.C.

    2015-01-01

    This thesis sheds light on the function and dysfunction of the protein α-synuclein (α-S) in the test tube and in cells and ultimately its possible involvement in Parkinson’s disease (PD). Following the introduction in Chapter 1, Chapters 2 and 3 concentrate on the investigation of the interaction

  16. Efficient particle-in-cell simulation of auroral plasma phenomena using a CUDA enabled graphics processing unit

    Science.gov (United States)

    Sewell, Stephen

    This thesis introduces a software framework that effectively utilizes low-cost commercially available Graphic Processing Units (GPUs) to simulate complex scientific plasma phenomena that are modeled using the Particle-In-Cell (PIC) paradigm. The software framework that was developed conforms to the Compute Unified Device Architecture (CUDA), a standard for general purpose graphic processing that was introduced by NVIDIA Corporation. This framework has been verified for correctness and applied to advance the state of understanding of the electromagnetic aspects of the development of the Aurora Borealis and Aurora Australis. For each phase of the PIC methodology, this research has identified one or more methods to exploit the problem's natural parallelism and effectively map it for execution on the graphic processing unit and its host processor. The sources of overhead that can reduce the effectiveness of parallelization for each of these methods have also been identified. One of the novel aspects of this research was the utilization of particle sorting during the grid interpolation phase. The final representation resulted in simulations that executed about 38 times faster than simulations that were run on a single-core general-purpose processing system. The scalability of this framework to larger problem sizes and future generation systems has also been investigated.

  17. Ecdysone Receptor-based Singular Gene Switches for Regulated Transgene Expression in Cells and Adult Rodent Tissues

    Directory of Open Access Journals (Sweden)

    Seoghyun Lee

    2016-01-01

    Full Text Available Controlled gene expression is an indispensable technique in biomedical research. Here, we report a convenient, straightforward, and reliable way to induce expression of a gene of interest with negligible background expression compared to the most widely used tetracycline (Tet-regulated system. Exploiting a Drosophila ecdysone receptor (EcR-based gene regulatory system, we generated nonviral and adenoviral singular vectors designated as pEUI(+ and pENTR-EUI, respectively, which contain all the required elements to guarantee regulated transgene expression (GAL4-miniVP16-EcR, termed GvEcR hereafter, and 10 tandem repeats of an upstream activation sequence promoter followed by a multiple cloning site. Through the transient and stable transfection of mammalian cell lines with reporter genes, we validated that tebufenozide, an ecdysone agonist, reversibly induced gene expression, in a dose- and time-dependent manner, with negligible background expression. In addition, we created an adenovirus derived from the pENTR-EUI vector that readily infected not only cultured cells but also rodent tissues and was sensitive to tebufenozide treatment for regulated transgene expression. These results suggest that EcR-based singular gene regulatory switches would be convenient tools for the induction of gene expression in cells and tissues in a tightly controlled fashion.

  18. Compensation of Wave-Induced Motion and Force Phenomena for Ship-Based High Performance Robotic and Human Amplifying Systems

    Energy Technology Data Exchange (ETDEWEB)

    Love, LJL

    2003-09-24

    The decrease in manpower and increase in material handling needs on many Naval vessels provides the motivation to explore the modeling and control of Naval robotic and robotic assistive devices. This report addresses the design, modeling, control and analysis of position and force controlled robotic systems operating on the deck of a moving ship. First we provide background information that quantifies the motion of the ship, both in terms of frequency and amplitude. We then formulate the motion of the ship in terms of homogeneous transforms. This transformation provides a link between the motion of the ship and the base of a manipulator. We model the kinematics of a manipulator as a serial extension of the ship motion. We then show how to use these transforms to formulate the kinetic and potential energy of a general, multi-degree of freedom manipulator moving on a ship. As a demonstration, we consider two examples: a one degree-of-freedom system experiencing three sea states operating in a plane to verify the methodology and a 3 degree of freedom system experiencing all six degrees of ship motion to illustrate the ease of computation and complexity of the solution. The first series of simulations explore the impact wave motion has on tracking performance of a position controlled robot. We provide a preliminary comparison between conventional linear control and Repetitive Learning Control (RLC) and show how fixed time delay RLC breaks down due to the varying nature wave disturbance frequency. Next, we explore the impact wave motion disturbances have on Human Amplification Technology (HAT). We begin with a description of the traditional HAT control methodology. Simulations show that the motion of the base of the robot, due to ship motion, generates disturbances forces reflected to the operator that significantly degrade the positioning accuracy and resolution at higher sea states. As with position-controlled manipulators, augmenting the control with a Repetitive

  19. Thermal systems; Systemes thermiques

    Energy Technology Data Exchange (ETDEWEB)

    Lalot, S. [Valenciennes Univ. et du Hainaut Cambresis, LME, 59 (France); Lecoeuche, S. [Ecole des Mines de Douai, Dept. GIP, 59 - Douai (France)]|[Lille Univ. des Sciences et Technologies, 59 - Villeneuve d' Ascq (France); Ahmad, M.; Sallee, H.; Quenard, D. [CSTB, 38 - Saint Martin d' Heres (France); Bontemps, A. [Universite Joseph Fourier, LEGI/GRETh, 38 - Grenoble (France); Gascoin, N.; Gillard, P.; Bernard, S. [Laboratoire d' Energetique, Explosion, Structure, 18 - Bourges (France); Gascoin, N.; Toure, Y. [Laboratoire Vision et Robotique, 18 - Bourges (France); Daniau, E.; Bouchez, M. [MBDA, 18 - Bourges (France); Dobrovicescu, A.; Stanciu, D. [Bucarest Univ. Polytechnique, Faculte de Genie Mecanique (Romania); Stoian, M. [Reims Univ. Champagne Ardenne, Faculte des Sciences, UTAP/LTM, 51 (France); Bruch, A.; Fourmigue, J.F.; Colasson, S. [CEA Grenoble, Lab. Greth, 38 (France); Bontemps, A. [Universite Joseph Fourier, LEGI/GRETh, 38 - Grenoble (France); Voicu, I.; Mare, T.; Miriel, J. [Institut National des Sciences Appliquees (INSA), LGCGM, IUT, 35 - Rennes (France); Galanis, N. [Sherbrooke Univ., Genie Mecanique, QC (Canada); Nemer, M.; Clodic, D. [Ecole des Mines de Paris, Centre Energetique et Procedes, 75 (France); Lasbet, Y.; Auvity, B.; Castelain, C.; Peerhossaini, H. [Nantes Univ., Ecole Polytechnique, Lab. de Thermocinetiquede Nantes, UMR-CNRS 6607, 44 (France)

    2005-07-01

    This session about thermal systems gathers 26 articles dealing with: neural model of a compact heat exchanger; experimental study and numerical simulation of the thermal behaviour of test-cells with walls made of a combination of phase change materials and super-insulating materials; hydraulic and thermal modeling of a supercritical fluid with pyrolysis inside a heated channel: pre-dimensioning of an experimental study; energy analysis of the heat recovery devices of a cryogenic system; numerical simulation of the thermo-hydraulic behaviour of a supercritical CO{sub 2} flow inside a vertical tube; mixed convection inside dual-tube exchangers; development of a nodal approach with homogenization for the simulation of the brazing cycle of a heat exchanger; chaotic exchanger for the cooling of low temperature fuel cells; structural optimization of the internal fins of a cylindrical generator; a new experimental approach for the study of the local boiling inside the channels of exchangers with plates and fins; experimental study of the flow regimes of boiling hydrocarbons on a bundle of staggered tubes; energy study of heat recovery exchangers used in Claude-type refrigerating systems; general model of Carnot engine submitted to various operating constraints; the free pistons Stirling cogeneration system; natural gas supplied cogeneration system with polymer membrane fuel cell; influence of the CRN coating on the heat flux inside the tool during the wood unrolling process; transport and mixture of a passive scalar injected inside the wake of a Ahmed body; control of a laser welding-brazing process by infrared thermography; 2D self-adaptative method for contours detection: application to the images of an aniso-thermal jet; exergy and exergy-economical study of an 'Ericsson' engine-based micro-cogeneration system; simplified air-conditioning of telephone switching equipments; parametric study of the 'low-energy' individual dwelling; brief synthesis of

  20. 6-Shogaol-Rich Extract from Ginger Up-Regulates the Antioxidant Defense Systems in Cells and Mice

    Directory of Open Access Journals (Sweden)

    Mira Jun

    2012-07-01

    Full Text Available The rhizome of ginger (Zingiber officinale Roscoe is known to have several bioactive compounds including gingerols and shogaols which possess beneficial health properties such as anti-inflammatory and chemopreventive effects. Based on recent observations that 6-shogaol may have more potent bioactivity than 6-gingerol, we obtained a 6-shogaol-rich extract from ginger and examined its effects on the nuclear factor E2-related factor2 (Nrf2/antioxidant response element (ARE pathway in vitro and in vivo. 6-Shogaol-rich extract was produced by extracting ginger powder with 95% ethanol at 80 °C after drying at 80 °C (GEE8080. GEE8080 contained over 6-fold more 6-shogaol compared to the room temperature extract (GEE80RT. In HepG2 cells, GEE8080 displayed much stronger inductions of ARE-reporter gene activity and Nrf2 expression than GEE80RT. GEE8080 stimulated phosphorylations of mitogen-activated protein kinases (MAPKs such as ERK, JNK, and p38. Moreover, the GEE8080-induced expressions of Nrf2 and HO-1 were attenuated by treatments of SB202190 (a p38 specific inhibitor and LY294002 (an Akt specific inhibitor. In a mouse model, the GEE8080 decreased the diethylnitrosamine (DEN-mediated elevations of serum aspartate transaminase and alanine transaminase as well as the DEN-induced hepatic lipid peroxidation. Inductions of Nrf2 and HO-1 by GEE8080 were also confirmed in the mice. In addition, the administration of GEE8080 to the mice also restored the DEN-reduced activity and protein expression of hepatic antioxidant enzymes such as superoxide dismutase, glutathione peroxidase and catalase. In conclusion, GEE8080, a 6-shogaol-rich ginger extract, may enhance antioxidant defense mechanism through the induction of Nrf2 and HO-1 regulated by p38 MAPK and PI3k/Akt pathway in vitro and in vivo.

  1. Antioxidant effectiveness of coffee extracts and selected constituents in cell-free systems and human colon cell lines.

    Science.gov (United States)

    Bakuradze, Tamara; Lang, Roman; Hofmann, Thomas; Stiebitz, Herbert; Bytof, Gerhard; Lantz, Ingo; Baum, Matthias; Eisenbrand, Gerhard; Janzowski, Christine

    2010-12-01

    Epidemiological studies suggest that coffee can reduce the risk of degenerative diseases such as diabetes type 2, cardiovascular disease and cancer. These beneficial effects have partly been attributed to the antioxidant activity of coffee. We determined composition and antioxidant potential of differentially roasted coffee extracts and investigated the impact of selected original constituents and roast products. Parameters studied were direct antioxidant activity (trolox equivalent antioxidant capacity/oxygen radical absorbing capacity), cellular reactive oxygen species (ROS) level, DNA damage and protein expression of NAD(P)H: quinone oxidoreductase, γ-glutamylcysteine ligase and glutathione reductase in HT-29/Caco-2 cells at 24-h incubation. All extracts showed distinct direct antioxidant activity: medium roasts>light roast AB1 (caffeoylquinic acid (CQA)-rich Arabica Brazil extract); dark roast AB2 (N-methylpyridinium (NMP)-rich Arabica Brazil extract), and diminished t-butylhydroperoxide-induced ROS level in HT-29 cells (AB2>medium roasts>AB1). NAD(P)H:quinone oxidoreductase 1 expression and γ-glutamylcysteine ligase expression were distinctly induced by AB1 and 5-CQA, but not by AB2 and NMP. 5-CQA and caffeic acid exhibited highest trolox equivalent antioxidant capacity/oxygen radical absorbing capacity values (5-CQA: 1.3/3.5 mM and caffeic acid: 1.3/3.9 mM trolox); ROS level was distinctly diminished by 5-CQA (≥3 μM), catechol (30 μM) and trigonelline (≥30 μM), whereas menadione-induced DNA damage in Caco-2 cells was reduced by NMP compounds (1-30 μM). The results emphasize that both original constituents and roast products contribute to the cellular antioxidant effectiveness of coffee. Copyright © 2010 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  2. Phenolic-enriched fractions from brewers' spent grain possess cellular antioxidant and immunomodulatory effects in cell culture model systems.

    Science.gov (United States)

    McCarthy, Aoife L; O'Callaghan, Yvonne C; Connolly, Alan; Piggott, Charles O; FitzGerald, Richard J; O'Brien, Nora M

    2014-05-01

    Large quantities of brewers' spent grain (BSG), a co-product of the brewing industry, are produced annually. BSG contains hydroxycinnamic acids, and phenolic-rich extracts from BSG have previously demonstrated the ability to protect against oxidant-induced DNA damage. The present study investigated the anti-inflammatory potential of eight phenolic extracts from BSG: four pale (P1-P4) and four black (B1-B4) extracts. BSG extracts were more cytotoxic in Jurkat T than U937 cells, with lower IC₅₀ values in Jurkat T cells, measured using the (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Pale BSG extracts P2 and P3 showed the greatest anti-inflammatory potential, significantly (P BSG, particularly the pale BSG extracts, have the ability to reduce a stimulated cytokine production and may also protect against cellular oxidative stress. Results of the present study highlight the potential of BSG phenolic extracts to act as functional food ingredients, providing an alternative use and improving the value of this brewing industry co-product. © 2013 Society of Chemical Industry.

  3. The Structural Basis of Action of Vanadyl (VO2+) Chelates in Cells.

    Science.gov (United States)

    Makinen, Marvin W; Salehitazangi, Marzieh

    2014-11-01

    Much emphasis has been given to vanadium compounds as potential therapeutic reagents for the treatment of diabetes mellitus. Thus far, no vanadium compound has proven efficacious for long-term treatment of this disease in humans. Therefore, in review of the research literature, our goal has been to identify properties of vanadium compounds that are likely to favor physiological and biochemical compatibility for further development as therapeutic reagents. We have, therefore, limited our review to those vanadium compounds that have been used in both in vivo experiments with small, laboratory animals and in in vitro studies with primary or cultured cell systems and for which pharmacokinetic and pharmacodynamics results have been reported, including vanadium tissue content, vanadium and ligand lifetime in the bloodstream, structure in solution, and interaction with serum transport proteins. Only vanadyl (VO 2+ ) chelates fulfill these requirements despite the large variety of vanadium compounds of different oxidation states, ligand structure, and coordination geometry synthesized as potential therapeutic agents. Extensive review of research results obtained with use of organic VO 2+ -chelates shows that the vanadyl chelate bis (acetylacetonato)oxidovanadium(IV) [hereafter abbreviated as VO(acac) 2 ], exhibits the greatest capacity to enhance insulin receptor kinase activity in cells compared to other organic VO 2+ -chelates, is associated with a dose-dependent capacity to lower plasma glucose in diabetic laboratory animals, and exhibits a sufficiently long lifetime in the blood stream to allow correlation of its dose-dependent action with blood vanadium content. The properties underlying this behavior appear to be its high stability and capacity to remain intact upon binding to serum albumin. We relate the capacity to remain intact upon binding to serum albumin to the requirement to undergo transcytosis through the vascular endothelium to gain access to target tissues

  4. Data Systems vs. Information Systems

    OpenAIRE

    Amatayakul, Margret K.

    1982-01-01

    This paper examines the current status of “hospital information systems” with respect to the distinction between data systems and information systems. It is proposed that the systems currently existing are incomplete data dystems resulting in ineffective information systems.

  5. Evaluation of diel patterns of relative changes in cell turgor of tomato plants using leaf patch clamp pressure probes

    NARCIS (Netherlands)

    Lee, K.M.; Driever, S.M.; Heuvelink, E.; Rüger, S.; Zimmermann, U.; Gelder, de A.; Marcelis, L.F.M.

    2012-01-01

    Relative changes in cell turgor of leaves of well-watered tomato plants were evaluated using the leaf patch clamp pressure probe (LPCP) under dynamic greenhouse climate conditions. Leaf patch clamp pressure changes, a measure for relative changes in cell turgor, were monitored at three different

  6. EXPERT SYSTEMS

    OpenAIRE

    Georgiana Marin; Mihai Catalin Andrei

    2011-01-01

    In recent decades IT and computer systems have evolved rapidly in economic informatics field. The goal is to create user friendly information systems that respond promptly and accurately to requests. Informatics systems evolved into decision assisted systems, and such systems are converted, based on gained experience, in expert systems for creative problem solving that an organization is facing. Expert systems are aimed at rebuilding human reasoning on the expertise obtained from experts, sto...

  7. Particle-in-cell simulations of plasma accelerators and electron-neutral collisions

    Directory of Open Access Journals (Sweden)

    David L. Bruhwiler

    2001-10-01

    Full Text Available We present 2D simulations of both beam-driven and laser-driven plasma wakefield accelerators, using the object-oriented particle-in-cell code XOOPIC, which is time explicit, fully electromagnetic, and capable of running on massively parallel supercomputers. Simulations of laser-driven wakefields with low \\(∼10^{16} W/cm^{2}\\ and high \\(∼10^{18} W/cm^{2}\\ peak intensity laser pulses are conducted in slab geometry, showing agreement with theory and fluid simulations. Simulations of the E-157 beam wakefield experiment at the Stanford Linear Accelerator Center, in which a 30 GeV electron beam passes through 1 m of preionized lithium plasma, are conducted in cylindrical geometry, obtaining good agreement with previous work. We briefly describe some of the more significant modifications to XOOPIC required by this work, and summarize the issues relevant to modeling relativistic electron-neutral collisions in a particle-in-cell code.

  8. In-cell thermodynamics and a new role for protein surfaces.

    Science.gov (United States)

    Smith, Austin E; Zhou, Larry Z; Gorensek, Annelise H; Senske, Michael; Pielak, Gary J

    2016-02-16

    There is abundant, physiologically relevant knowledge about protein cores; they are hydrophobic, exquisitely well packed, and nearly all hydrogen bonds are satisfied. An equivalent understanding of protein surfaces has remained elusive because proteins are almost exclusively studied in vitro in simple aqueous solutions. Here, we establish the essential physiological roles played by protein surfaces by measuring the equilibrium thermodynamics and kinetics of protein folding in the complex environment of living Escherichia coli cells, and under physiologically relevant in vitro conditions. Fluorine NMR data on the 7-kDa globular N-terminal SH3 domain of Drosophila signal transduction protein drk (SH3) show that charge-charge interactions are fundamental to protein stability and folding kinetics in cells. Our results contradict predictions from accepted theories of macromolecular crowding and show that cosolutes commonly used to mimic the cellular interior do not yield physiologically relevant information. As such, we provide the foundation for a complete picture of protein chemistry in cells.

  9. Electromagnetic particle in cell modeling of the plasma focus: Current sheath formation and lift off

    International Nuclear Information System (INIS)

    Seng, Y. S.; Lee, P.; Rawat, R. S.

    2014-01-01

    The shaping and formation of the current sheath takes place in the breakdown phase of a plasma focus device and critically controls the device performance. Electrostatic particle in cell codes, with magnetic effects ignored, have been used to model the breakdown phase. This Letter reports the successful development and implementation of an electromagnetic particle in cell (EMPIC) code, including magnetic effects self-consistently, to simulate the breakdown phase; from the ionization, localization and gliding discharge along the insulator to the time instant of current sheath lift off. The magnetic field was found to be appreciable from the time the current sheath came into contact with the anode with increased local current, initiating the voltage breakdown of the device as a result

  10. Multibody Systems

    DEFF Research Database (Denmark)

    Wagner, Falko Jens

    1999-01-01

    Multibody Systems is one area, in which methods for solving DAEs are of special interst. This chapter is about multibody systems, why they result in DAE systems and what kind of problems that can arise when dealing with multibody systems and formulating their corresponding DAE system....

  11. Fractional variational problems and particle in cell gyrokinetic simulations with fuzzy logic approach for tokamaks

    Directory of Open Access Journals (Sweden)

    Rastović Danilo

    2009-01-01

    Full Text Available In earlier Rastovic's papers [1] and [2], the effort was given to analyze the stochastic control of tokamaks. In this paper, the deterministic control of tokamak turbulence is investigated via fractional variational calculus, particle in cell simulations, and fuzzy logic methods. Fractional integrals can be considered as approximations of integrals on fractals. The turbulent media could be of the fractal structure and the corresponding equations should be changed to include the fractal features of the media.

  12. Improved Conservation Properties for Particle-in-cell Simulations with Kinetic Electrons

    International Nuclear Information System (INIS)

    Lewandowski, J.L.V.

    2003-01-01

    It is shown that a simple algorithm which exactly segregates between adiabatic and non-adiabatic electrons in particle-in-cell simulations of drift modes yields excellent conservation properties (e.g. particle number, energy) compared to the conventional df scheme. The removal of the free streaming term in the evolution of the marker weight is shown to be responsible for the improved linear and nonlinear properties of the simulated plasma

  13. BACE-1 inhibitors part 3: identification of hydroxy ethylamines (HEAs) with nanomolar potency in cells.

    Science.gov (United States)

    Beswick, Paul; Charrier, Nicolas; Clarke, Brian; Demont, Emmanuel; Dingwall, Colin; Dunsdon, Rachel; Faller, Andrew; Gleave, Robert; Hawkins, Julie; Hussain, Ishrut; Johnson, Christopher N; MacPherson, David; Maile, Graham; Matico, Rosalie; Milner, Peter; Mosley, Julie; Naylor, Alan; O'Brien, Alistair; Redshaw, Sally; Riddell, David; Rowland, Paul; Skidmore, John; Soleil, Virginie; Smith, Kathrine J; Stanway, Steven; Stemp, Geoffrey; Stuart, Alistair; Sweitzer, Sharon; Theobald, Pam; Vesey, David; Walter, Daryl S; Ward, John; Wayne, Gareth

    2008-02-01

    This article is focusing on further optimization of previously described hydroxy ethylamine (HEA) BACE-1 inhibitors obtained from a focused library with the support of X-ray crystallography. Optimization of the non-prime side of our inhibitors and introduction of a 6-membered sultam substituent binding to Asn-294 as well as a fluorine in the C-2 position led to derivatives with nanomolar potency in cell-based assays.

  14. Cholesterol metabolism: use of D2O for determination of synthesis rate in cell culture

    International Nuclear Information System (INIS)

    Esterman, A.L.; Cohen, B.I.; Javitt, N.B.

    1985-01-01

    Cholesterol synthesis in cell culture in the presence of D 2 O yields a spectrum of enriched molecules having a relative abundance that indicates random substitution of deuterium for hydrogen. Quantitation of the absolute rate of cholesterol synthesis is obtained by isotope ratio mass spectrometry. Mevinolin and 26-hydroxycholesterol both decrease cholesterol synthesis rate but have a discordant effect on HMG-CoA reductase activity

  15. Particle-In-Cell/Monte Carlo Simulation of Ion Back Bombardment in Photoinjectors

    International Nuclear Information System (INIS)

    Qiang, Ji; Corlett, John; Staples, John

    2009-01-01

    In this paper, we report on studies of ion back bombardment in high average current dc and rf photoinjectors using a particle-in-cell/Monte Carlo method. Using H 2 ion as an example, we observed that the ion density and energy deposition on the photocathode in rf guns are order of magnitude lower than that in a dc gun. A higher rf frequency helps mitigate the ion back bombardment of the cathode in rf guns

  16. Radiation hardening and irradiation testing of in-cell electronics for MA23/APM

    International Nuclear Information System (INIS)

    Friant, A.

    1988-09-01

    We relate briefly the radiation hardening method used to guarantee a gamma resistance of 10 Mrad for the whole electronic equipment associated with the slave arm of MA23 M servomanipulator which will be set up in cell 404 in Marcoule (APM). We describe the radiation testing of electronic devices and of the various subsystems designed by the D. LETI groups involved in the MA23/APM project

  17. Long-term luminescence of organic dyes in cells of biological tissues

    Science.gov (United States)

    Letuta, S. N.; Maryakhina, V. S.; Pashkevich, S. N.; Rakhmatullin, R. R.

    2011-01-01

    We studied the kinetics of delayed fluorescence and phosphoresce of exogenous fluorophores in cells extracted from tumorous and normal tissues of the mammary gland of mice of the BYRB strain. We revealed the specifics and determined regularities of the kinetics of long-term luminescence of fluorophores in different cells. The possibility of developing of a method for the early fluorescence diagnostics of the pathology of biotissues based on the obtained results is discussed.

  18. Swelling-activated ion channels: functional regulation in cell-swelling, proliferation and apoptosis

    DEFF Research Database (Denmark)

    Stutzin, A; Hoffmann, E K

    2006-01-01

    Cell volume regulation is one of the most fundamental homeostatic mechanisms and essential for normal cellular function. At the same time, however, many physiological mechanisms are associated with regulatory changes in cell size meaning that the set point for cell volume regulation is under phys...... as key players in the maintenance of normal steady-state cell volume, with particular emphasis on the intracellular signalling pathways responsible for their regulation during hypotonic stress, cell proliferation and apoptosis....

  19. A Thiopurine Drug Inhibits West Nile Virus Production in Cell Culture, but Not in Mice

    OpenAIRE

    Lim, Pei-Yin; Keating, Julie A.; Hoover, Spencer; Striker, Rob; Bernard, Kristen A.

    2011-01-01

    Many viruses within the Flavivirus genus cause significant disease in humans; however, effective antivirals against these viruses are not currently available. We have previously shown that a thiopurine drug, 6-methylmercaptopurine riboside (6MMPr), inhibits replication of distantly related viruses within the Flaviviridae family in cell culture, including bovine viral diarrhea virus and hepatitis C virus replicon. Here we further examined the potential antiviral effect of 6MMPr on several dive...

  20. Recent advances in cell-free PrPSc amplification technique.

    Science.gov (United States)

    Atarashi, Ryuichiro

    2009-01-01

    The development of amplification technology for abnormal forms of prion protein in vitro has had a great impact on the field of prion research. This novel technology has generated new possibilities for understanding the molecular basis of prions and for developing an early diagnostic test for prion diseases. This review provides an overview of recent progress in cell-free PrPSc amplification techniques.

  1. Paclitaxel stimulates chromosomal fusion and instability in cells with dysfunctional telomeres: Implication in multinucleation and chemosensitization

    Energy Technology Data Exchange (ETDEWEB)

    Park, Jeong-Eun [Division of Radiation Cancer Research, Korea Institute of Radiological and Medical Sciences, Seoul 139-706 (Korea, Republic of); Woo, Seon Rang [Division of Radiation Cancer Research, Korea Institute of Radiological and Medical Sciences, Seoul 139-706 (Korea, Republic of); Department of Biochemistry, College of Medicine, Korea University, Seoul 136-705 (Korea, Republic of); Kang, Chang-Mo [Laboratory of Cytogenetics and Tissue Regeneration, Korea Institute of Radiological and Medical Sciences, Seoul 139-706 (Korea, Republic of); Juhn, Kyoung-Mi; Ju, Yeun-Jin; Shin, Hyun-Jin; Joo, Hyun-Yoo; Park, Eun Ran; Park, In-chul; Hong, Sung Hee; Hwang, Sang-Gu [Division of Radiation Cancer Research, Korea Institute of Radiological and Medical Sciences, Seoul 139-706 (Korea, Republic of); Lee, Jung-Kee [Department of Life Science and Genetic Engineering, Paichai University, Daejeon 302-735 (Korea, Republic of); Kim, Hae Kwon [Department of Biotechnology, Seoul Woman' s University, Seoul 139-774 (Korea, Republic of); Cho, Myung-Haing [Laboratory of Toxicology, College of Veterinary Medicine, Seoul National University, Seoul 151-74-2 (Korea, Republic of); Park, Gil Hong [Department of Biochemistry, College of Medicine, Korea University, Seoul 136-705 (Korea, Republic of); Lee, Kee-Ho, E-mail: khlee@kirams.re.kr [Division of Radiation Cancer Research, Korea Institute of Radiological and Medical Sciences, Seoul 139-706 (Korea, Republic of)

    2011-01-14

    Research highlights: {yields} Paclitaxel serves as a stimulator of chromosomal fusion in cells in which telomeres are dysfunctional. {yields} Typical fusions involve p-arms, but paclitaxel-induced fusions occur between both q- and p-arms. {yields} Paclitaxel-stimulated fusions in cells in which telomeres are dysfunctional evoke prolonged G2/M cell cycle arrest and delay multinucleation. {yields} Upon telomere erosion, paclitaxel promotes chromosomal instability and subsequent apoptosis. {yields} Chromosomal fusion enhances paclitaxel chemosensitivity under telomere dysfunction. -- Abstract: The anticancer effect of paclitaxel is attributable principally to irreversible promotion of microtubule stabilization and is hampered upon development of chemoresistance by tumor cells. Telomere shortening, and eventual telomere erosion, evoke chromosomal instability, resulting in particular cellular responses. Using telomerase-deficient cells derived from mTREC-/-p53-/- mice, here we show that, upon telomere erosion, paclitaxel propagates chromosomal instability by stimulating chromosomal end-to-end fusions and delaying the development of multinucleation. The end-to-end fusions involve both the p- and q-arms in cells in which telomeres are dysfunctional. Paclitaxel-induced chromosomal fusions were accompanied by prolonged G2/M cell cycle arrest, delayed multinucleation, and apoptosis. Telomere dysfunctional cells with mutlinucleation eventually underwent apoptosis. Thus, as telomere erosion proceeds, paclitaxel stimulates chromosomal fusion and instability, and both apoptosis and chemosensitization eventually develop.

  2. Small-molecule-based inhibition of histone demethylation in cells assessed by quantitative mass spectrometry.

    Science.gov (United States)

    Mackeen, Mukram M; Kramer, Holger B; Chang, Kai-Hsuan; Coleman, Matthew L; Hopkinson, Richard J; Schofield, Christopher J; Kessler, Benedikt M

    2010-08-06

    Post-translational modifications on histones are an important mechanism for the regulation of gene expression and are involved in all aspects of cell growth and differentiation, as well as pathological processes including neurodegeneration, autoimmunity, and cancer. A major challenge within the chromatin field is to develop methods for the quantitative analysis of histone modifications. Here we report a mass spectrometry (MS) approach based on ultraperformance liquid chromatography high/low collision switching (UPLC-MS(E)) to monitor histone modifications in cells. This approach is exemplified by the analysis of trimethylated lysine-9 levels in histone H3, following a simple chemical derivatization procedure with d(6)-acetic anhydride. This method was used to study the inhibition of histone demethylases with pyridine-2,4-dicarboxylic acid (PDCA) derivatives in cells. Our results show that the PDCA-dimethyl ester inhibits JMJD2A catalyzed demethylation of lysine-9 on histone H3 in human HEK 293T cells. Demethylase inhibition, as observed by MS analyses, was supported by immunoblotting with modification-specific antibodies. The results demonstrate that PDCA derived small molecules are cell permeable demethylase inhibitors and reveal that quantitative MS is a useful tool for measuring post-translational histone modifications in cells.

  3. Introduction to the Minireview Series on Modern Technologies for In-cell Biochemistry.

    Science.gov (United States)

    Lutsenko, Svetlana

    2016-02-19

    The last decade has seen enormous progress in the exploration and understanding of the behavior of molecules in their natural cellular environments at increasingly high spatial and temporal resolution. Advances in microscopy and the development of new fluorescent reagents as well as genetic editing techniques have enabled quantitative analysis of protein interactions, intracellular trafficking, metabolic changes, and signaling. Modern biochemistry now faces new and exciting challenges. Can traditionally "in vitro" experiments, e.g. analysis of protein folding and conformational transitions, be done in cells? Can the structure and behavior of endogenous and/or non-tagged recombinant proteins be analyzed and altered within the cell or in cellular compartments? How can molecules and their actions be studied mechanistically in tissues and organs? Is personalized cellular biochemistry a reality? This thematic series summarizes recent studies that illustrate some first steps toward successfully answering these modern biochemical questions. The first minireview focuses on utilization of three-dimensional primary enteroids and organoids for mechanistic studies of intestinal biology with molecular resolution. The second minireview describes application of single chain antibodies (nanobodies) for monitoring and regulating protein dynamics in vitro and in cells. The third minireview highlights advances in using NMR spectroscopy for analysis of protein folding and assembly in cells. © 2016 by The American Society for Biochemistry and Molecular Biology, Inc.

  4. Electromagnetic fields in biological systems

    CERN Document Server

    Lin, James C

    2016-01-01

    As wireless technology becomes more sophisticated and accessible to more users, the interactions of electromagnetic fields with biological systems have captured the interest not only of the scientific community but also the general public. Unintended or deleterious biological effects of electromagnetic fields and radiation may indicate grounds for health and safety precautions in their use. Spanning static fields to terahertz waves, Electromagnetic Fields in Biological Systems explores the range of consequences these fields have on the human body. With contributions by an array of experts, topics discussed include: Essential interactions and field coupling phenomena, highlighting their importance in research on biological effects and in scientific, industrial, and medical applications Electric field interactions in cells, focusing on ultrashort, pulsed high-intensity fields The effect of exposure to naturally occurring and human-made static, low-frequency, and pulsed magnetic fields in biological systems Dosi...

  5. Closure system

    International Nuclear Information System (INIS)

    Kube, L.J.

    1978-01-01

    This invention relates generally to gas-cooled nuclear reactor systems and, more particularly, to an improved closure system for a pressure vessel in such a system wherein a penetration is provided for accommodating a heat exchanger. (author)

  6. Lymph system

    Science.gov (United States)

    Lymphatic system ... neck, under the arms, and groin. The lymph system includes the: Tonsils Adenoids Spleen Thymus ... JE, Flynn JA, Solomon BS, Stewart RW. Lymphatic system. In: Ball JW, Dains JE, Flynn JA, Solomon ...

  7. Study of self-consistent particle flows in a plasma blob with particle-in-cell simulations

    Energy Technology Data Exchange (ETDEWEB)

    Hasegawa, Hiroki, E-mail: hasegawa.hiroki@nifs.ac.jp; Ishiguro, Seiji [Department of Helical Plasma Research, National Institute for Fusion Science, Toki 509-5292 (Japan); Department of Fusion Science, SOKENDAI (The Graduate University for Advanced Studies), Toki 509-5292 (Japan)

    2015-10-15

    The self-consistent particle flows in a filamentary coherent structure along the magnetic field line in scrape-off layer (SOL) plasma (plasma blob) have been investigated by means of a three-dimensional electrostatic particle-in-cell simulation code. The presence of the spiral current system composed of the diamagnetic and parallel currents in a blob is confirmed by the particle simulation without any assumed sheath boundary models. Furthermore, the observation of the electron and ion parallel velocity distributions in a blob shows that those distributions are far from Maxwellian due to modification with the sheath formation and that the electron temperature on the higher potential side in a blob is higher than that on the lower potential side. Also, it is found that the ions on the higher potential side are accelerated more intensively along the magnetic field line than those on the lower potential side near the edge. This study indicates that particle simulations are able to provide an exact current closure to analysis of blob dynamics and will bring more accurate prediction of plasma transport in the SOL without any empirical assumptions.

  8. PICK1 expression in the Drosophila central nervous system primarily occurs in the neuroendocrine system

    DEFF Research Database (Denmark)

    Jansen, Anna M; Nässel, Dick R; Madsen, Kenneth L

    2009-01-01

    in the adult and larval Drosophila central nervous system. PICK1 was found in cell bodies in the subesophageal ganglion, the antennal lobe, the protocerebrum, and the neuroendocrine center pars intercerebralis. The cell types that express PICK1 were identified using GAL4 enhancer trap lines. The PICK1...... (AMPA) receptor subunit GluR2 and the dopamine transporter. PICK1 is strongly implicated in GluR2 trafficking and synaptic plasticity. In mammals, PICK1 has been characterized extensively in cell culture studies. To study PICK1 in an intact system, we characterized PICK1 expression immunohistochemically...... neurons in the neuroendocrine system, which express the transcription factor DIMM and the amidating enzyme peptidylglycine-alpha-hydroxylating monooxygenase (PHM). The PICK1-positive cells include neurosecretory cells that produce the insulin-like peptide dILP2. PICK1 expression in insulin-producing cells...

  9. Phosphorylation of protein kinase A (PKA) regulatory subunit RIα by protein kinase G (PKG) primes PKA for catalytic activity in cells.

    Science.gov (United States)

    Haushalter, Kristofer J; Casteel, Darren E; Raffeiner, Andrea; Stefan, Eduard; Patel, Hemal H; Taylor, Susan S

    2018-03-23

    cAMP-dependent protein kinase (PKAc) is a pivotal signaling protein in eukaryotic cells. PKAc has two well-characterized regulatory subunit proteins, RI and RII (each having α and β isoforms), which keep the PKAc catalytic subunit in a catalytically inactive state until activation by cAMP. Previous reports showed that the RIα regulatory subunit is phosphorylated by cGMP-dependent protein kinase (PKG) in vitro , whereupon phosphorylated RIα no longer inhibits PKAc at normal (1:1) stoichiometric ratios. However, the significance of this phosphorylation as a mechanism for activating type I PKA holoenzymes has not been fully explored, especially in cellular systems. In this study, we further examined the potential of RIα phosphorylation to regulate physiologically relevant "desensitization" of PKAc activity. First, the serine 101 site of RIα was validated as a target of PKGIα phosphorylation both in vitro and in cells. Analysis of a phosphomimetic substitution in RIα (S101E) showed that modification of this site increases PKAc activity in vitro and in cells, even without cAMP stimulation. Numerous techniques were used to show that although Ser 101 variants of RIα can bind PKAc, the modified linker region of the S101E mutant has a significantly reduced affinity for the PKAc active site. These findings suggest that RIα phosphorylation may be a novel mechanism to circumvent the requirement of cAMP stimulus to activate type I PKA in cells. We have thus proposed a model to explain how PKG phosphorylation of RIα creates a "sensitized intermediate" state that is in effect primed to trigger PKAc activity.

  10. Gibberellin biosynthesis in cell-free extracts from developing Cucurbita maxima embryos and the identification of new endogenous gibberellins.

    Science.gov (United States)

    Lange, T; Hedden, P; Graebe, J E

    1993-03-01

    Gibberellin (GA) biosynthetic pathways from GA12-aldehyde, GA12 and GA53 were investigated in cell-free systems from developing embryos of Cucurbita maxima L. Gibberellin A12-aldehyde and GA12 were converted to GA25, putative 12α-hydroxyGA25, GA13 and GA39 as main products. Minor products were GA4, GA34 and, when GA12 was the substrate, putative 12α-hydroxyGA12. The intermediates GA15 and GA24 accumulated at low protein concentrations. The influence of various factors on GA12 metabolism was examined. At low 2-oxoglutarate and ascorbate concentrations, or at acid pH, 3β-hydroxylated products predominated, whereas with increasing 2-oxoglutarate and ascorbate concentrations, or at neutral pH, the yield of 12α-hydroxylated GAs increased. Gibberellin A53 was metabolised mainly to the C20-GAs GA44, GA19, GA17, GA23 and GA28, with the C19-GAs GA20, GA1 and GA8 as minor products. Only C19-GAs were 2β-hydroxylated, which is a main characteristic of the embryo systems. In addition to GA13, GA25, GA39, GA43, GA49, GA58, GA74, 12α-hydroxyGA25 and GA39 3-isovalerate, which were known previously from embryos of C. maxima, GA1, GA4, GA17, GA28, GA37, GA38, GA48, GA85, 12α-hydroxyGA37 and putative 12α-hydroxyGA43 were identified as endogenous components by full-scan capillary gas chromatography-mass spectrometry and Kovats retention indices. Evidence for putative 2β-hydroxyGA28 and GA23 was also obtained but it was less conclusive because of contamination.

  11. INIS system

    International Nuclear Information System (INIS)

    Sokalski, A.

    1982-01-01

    History, organizational structure and operation principles of INIS system are presented. The preparation of input, checking and data processing as well as output production, computer forms of files and information retrieval systems are described in detail. The active participation of Poland in the system is emphasized. The possible ways of system development are presented. (author)

  12. Systems effectiveness

    CERN Document Server

    Habayeb, A R

    1987-01-01

    Highlights three principal applications of system effectiveness: hardware system evaluation, organizational development and evaluation, and conflict analysis. The text emphasizes the commonality of the system effectiveness discipline. The first part of the work presents a framework for system effectiveness, partitioning and hierarchy of hardware systems. The second part covers the structure, hierarchy, states, functions and activities of organizations. Contains an extended Appendix on mathematical concepts and also several project suggestions.

  13. Reactive Systems

    DEFF Research Database (Denmark)

    Aceto, Luca; Ingolfsdottir, Anna; Larsen, Kim Guldstrand

    A reactive system comprises networks of computing components, achieving their goals through interaction among themselves and their environment. Thus even relatively small systems may exhibit unexpectedly complex behaviours. As moreover reactive systems are often used in safety critical systems......, the need for mathematically based formal methodology is increasingly important. There are many books that look at particular methodologies for such systems. This book offers a more balanced introduction for graduate students and describes the various approaches, their strengths and weaknesses, and when...

  14. Nonequilibrium Gyrokinetic Fluctuation Theory and Sampling Noise in Gyrokinetic Particle-in-cell Simulations

    International Nuclear Information System (INIS)

    Krommes, John A.

    2007-01-01

    The present state of the theory of fluctuations in gyrokinetic (GK) plasmas and especially its application to sampling noise in GK particle-in-cell (PIC) simulations is reviewed. Topics addressed include the Δf method, the fluctuation-dissipation theorem for both classical and GK many-body plasmas, the Klimontovich formalism, sampling noise in PIC simulations, statistical closure for partial differential equations, the theoretical foundations of spectral balance in the presence of arbitrary noise sources, and the derivation of Kadomtsev-type equations from the general formalism

  15. [The time course of changes in cell immunological parameters during administration of live dry plague vaccine].

    Science.gov (United States)

    Bogacheva, N V; Darmov, I V; Borisevich, I V; Kriuchkov, A V; Pechenkin, D V

    2009-08-01

    The study of the time course of changes in cell immunological parameters by a magnetic separation technique in human beings during the administration of plague vaccine in relation to the immunological load revealed the higher blood levels of all T lymphocyte subpopulations on day 14 after vaccination. These changes are most typical of a primary vaccinated cohort. The increased frequency of plague vaccine administration and multiple immunizations with live plague, anthrax, and tularemia vaccines produce the time-course of changes in T lymphocyte populations (subpopulations) in response to the regular administration of plague vaccine. A high immunological load in man also promotes a significant reduction in the level of B lymphocytes.

  16. Particle-in-Cell Code BEAMPATH for Beam Dynamics Simulations in Linear Accelerators and Beamlines

    Energy Technology Data Exchange (ETDEWEB)

    Batygin, Y.

    2004-10-28

    A code library BEAMPATH for 2 - dimensional and 3 - dimensional space charge dominated beam dynamics study in linear particle accelerators and beam transport lines is developed. The program is used for particle-in-cell simulation of axial-symmetric, quadrupole-symmetric and z-uniform beams in a channel containing RF gaps, radio-frequency quadrupoles, multipole lenses, solenoids and bending magnets. The programming method includes hierarchical program design using program-independent modules and a flexible combination of modules to provide the most effective version of the structure for every specific case of simulation. Numerical techniques as well as the results of beam dynamics studies are presented.

  17. Finite element approach to global gyrokinetic particle-in-cell simulations using magnetic coordinate

    International Nuclear Information System (INIS)

    Fivaz, M.; Brunner, S.; Ridder, G. de; Sauter, O.; Tran, T.M.; Vaclavik, J.; Villard, L.; Appert, K.

    1997-08-01

    We present a fully-global linear gyrokinetic simulation code (GYGLES) aimed at describing the instable spectrum of the ion-temperature-gradient modes in toroidal geometry. We formulate the Particle-In-Cell method with finite elements defined in magnetic coordinates, which provides excellent numerical convergence properties. The poloidal mode structure corresponding to k // =0 is extracted without approximation from the equations, which reduces drastically the numerical resolution needed. The code can simulate routinely modes with both very long and very short toroidal wavelengths, can treat realistic (MHD) equilibria of any size and runs on a massively parallel computer. (author) 10 figs., 28 refs

  18. The cytoskeleton in cell-autonomous immunity: structural determinants of host defence

    Science.gov (United States)

    Mostowy, Serge; Shenoy, Avinash R.

    2016-01-01

    Host cells use antimicrobial proteins, pathogen-restrictive compartmentalization and cell death in their defence against intracellular pathogens. Recent work has revealed that four components of the cytoskeleton — actin, microtubules, intermediate filaments and septins, which are well known for their roles in cell division, shape and movement — have important functions in innate immunity and cellular self-defence. Investigations using cellular and animal models have shown that these cytoskeletal proteins are crucial for sensing bacteria and for mobilizing effector mechanisms to eliminate them. In this Review, we highlight the emerging roles of the cytoskeleton as a structural determinant of cell-autonomous host defence. PMID:26292640

  19. Membrane tension: A challenging but universal physical parameter in cell biology.

    Science.gov (United States)

    Pontes, Bruno; Monzo, Pascale; Gauthier, Nils C

    2017-11-01

    The plasma membrane separates the interior of cells from the outside environment. The membrane tension, defined as the force per unit length acting on a cross-section of membrane, regulates many vital biological processes. In this review, we summarize the first historical findings and the latest advances, showing membrane tension as an important physical parameter in cell biology. We also discuss how this parameter must be better integrated and we propose experimental approaches for key unanswered questions. Copyright © 2017 Elsevier Ltd. All rights reserved.

  20. 3-D particle-in-cell simulation of laser-produced plasma in axial magnetic field

    Czech Academy of Sciences Publication Activity Database

    Roy, Amitava; Endo, Akira; Mocek, Tomáš

    2016-01-01

    Roč. 44, č. 4 (2016), s. 574-581 ISSN 0093-3813 R&D Projects: GA MŠk ED2.1.00/01.0027; GA MŠk EE2.3.20.0143 Grant - others:HILASE(XE) CZ.1.05/2.1.00/01.0027; OP VK 6(XE) CZ.1.07/2.3.00/20.0143 Institutional support: RVO:68378271 Keywords : laser-produced plasma (LPP) * magnetized plasma * particle-in-cell (PIC) simulation * plasma instability Subject RIV: BH - Optics, Masers, Lasers Impact factor: 1.052, year: 2016