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Sample records for i-123 imp spect

  1. The study of I-123-IMP-SPECT before and after surgery for craniosynostosis

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    Satoh, Motohiro (University Hospital of Tsukuba, Ibaraki (Japan)); Ishikawa, Nobuyoshi; Enomoto, Takao; Takeda, Tohru; Yoshizawa, Takashi; Nose, Tadao

    1990-12-01

    Single photon emission computed tomography with N-isopropyl-p-(I-123)iodoamphetamine was performed in 13 cases of craniosynostosis before and after surgery. Of 13 cases, 8 cases (62%) showed focal low perfusion area on preoperative study. Four of seven cases (57%) with brachycephaly showed low perfusion areas in either of frontal lobes, occipital lobes, and cerebellum. Besides, two patients with scaphocephaly and one with plagiocephaly showed low perfusion area in the unilateral cerebral hemisphere. Two Crouzon disease cases showed no focal low perfusion area, but an Apert disease showed low perfusion areas in both frontal lobes, cerebellum, as well as left occipital lobe. Corresponding CT and MRI showed no focal abnormality in any of these cases. These low perfusion areas were diminished or disappeared after surgical treatment in 6 cases (75%). We conclude that the I-123-IMP-SPECT is considered to be a useful index for the evaluation of functional recovery after surgery in cases of craniosynostosis. (author).

  2. The group study of diagnostic efficacy of cerebro-vascular disease by I-123 IMP SPECT images obtained with ring type SPECT scanner; The ROC analysis on the diagnosis of perfusion defect and redistribution

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    Machida, Kikuo; Honda, Norinari (Saitama Medical School, Kawagoe (Japan). Saitama Medical Center); Matsumoto, Toru (and others)

    1991-11-01

    We performed two image reading experiments in order to investigate the diagnostic capability of I-123 IMP SPECT obtained by the ring type SPECT scanner in cerebro-vascular disease. Fourteen physicians diagnosed SPECT images of 55 cases with reference to clinical neurological information, first without brain XCT images and second with XCT images. Each physician detected perfusion defects and redistributions of I-123 IMP and assigned a confidence level of abnormality for these SPECT findings by means of five rating method. From results obtained by ROC analysis, we concluded as follows. (1) Generally, I-123 IMP SPECT is a stable diagnostic modality in the diagnosis of cerebro-vascular disease and the image reading of XCT had no effects on the diagnosis of SPECT on the whole of physician. (2) However, there were unnegligible differences among individuals in the detectability of findings and the effect of XCT image reading. (3) Detectability of redistribution of I-123 IMP was lower than that of perfusion defect and inter-observer variation in the diagnostic performance for redistribution was larger than that of perfusion defect. The results suggest that it is necessary to standardize diagnostic criteria among physicians for redistribution of I-123 IMP. (author).

  3. Evaluation of crossed cerebellar diaschisis in 30 patients with major cerebral artery occlusion by means of quantitative I-123 IMP SPECT

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    Miyazawa, Nobuhiko; Toyama, Keiji; Arbab, A.S.; Arai, Takao; Nukui, Hideaki [Yamanashi Medical Univ., Tamaho (Japan); Koizumi, Kiyoshi

    2001-12-01

    Quantitative crossed cerebellar diaschisis (CCD) and the correlation with a reduction in supratentorial regional cerebral blood flow (rCBF) and cerebrovascular reserve capacity (CVR) were investigated in clinically stable patients with major cerebral artery occlusion by the iodine-123-N-isopropyl-p-iodoamphetamine (I-123 IMP) single photon emission computed tomography (SPECT) method. Thirty patients with major cerebral artery occlusion underwent SPECT by the I-123 IMP autoradiographic method. Regional CBF was measured in the cerebral hemisphere, frontal and parietal lobes, temporo-parietal lobe, and cerebellum both at rest and after administration of acetazolamide. Eighteen of 30 patients (60%) had CCD. CCD was significantly related to magnetic resonance imaging evidence of infarction. Quantitative CCD was 17% and the CVR in the cerebellum was preserved in patients with CCD. There was a significant difference in CBF and CVR between the affected and normal sides in all regions of interest in the patients without CCD [CBF (ml/100 g/min): hemisphere (H), normal side (N): 31.4{+-}6.8, affected side (A): 27.5{+-}7.4; p<0.05. CVR: H, N: 0.56{+-}0.38, A: 0.42{+-}0.18; p<0.01]. CCD is common in patients with major cerebral artery occlusion, and quantitative I-123 IMP SPECT is helpful in detecting CCD in clinically stable patients with occlusion of major cerebral arteries. (author)

  4. Asymmetry of cerebral blood flow in patients with senile dementia of Alzheimer type by SPECT using I-123 IMP

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    Arai, Hisayuki; Hanyu, Haruo; Kobayashi, Yasutaka; Hatano, Nobuyoshi; Katsunuma, Hideyo; Suzuki, Takanari; Amino, Saburo (Tokyo Medical Coll. (Japan))

    1990-12-01

    Regional cerebral blood flow (rCBF) was determined by single photon emission CT (SPECT) with N-isopropyl-p-({sup 123}I)iodoamphetamine in 22 patients with clinically diagnosed senile dementia of Alzheimer type (SDAT) and in 18 age matched controls. We calculated asymmetry indices (AIs) of rCBF for matched right-left regions of interest. rCBF of parietal lobe in SDAT patients was significantly most laterally asymmetrycal, but the least in occipital lobe. Lateral asymmetry of rCBF in SDAT patients correlated with asymmetry of language and visuospatial functions; decreased rCBF in the left parietal lobe was associated with language dysfunction, and that in the right parietal lobe, with viduospatial dysfunction. Furthermore cerebellar AIs correlated negatively with those of the cerebral hemisphere and lower frontal region in SDAT patients. The results demonstrate that rCBF measurement by {sup 123}I-IMP SPECT is useful to detect lateral asymmetry in reduction of rCBF in SDAT. (author).

  5. Tc-99m-HMPAO brain scintigraphy. A comparative study with I-123-IMP

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    Nishimoto, Hitoshi; Yamamoto, Yoichi; Yoshida, Shoji; Akagi, Naoki; Ohara, Shuichi; Kamiike, Osamu; Maeda, Tomoho

    1989-04-01

    Sixteen single photon emission computed tomography (SPECT) studies with Tc-99m hexamethyl propylene amine oxime (Tc-99m HMPAO) were performed in patients with cerebral infarction (n=10), transient ischemic attack (n=2), or brain tumor (n=3). X-ray computed tomography (CT) scans and SPECT scans with N-isopropyl-p-(I-123)iodoamphetamine (I-123 IMP) were also obtained for comparison with findings of SPECT with Tc-99m HMPAO. Crossed cerebellar diaschisis (CCD) was detected in five patients (33%) on both early and delayed SPECT scans with Tc-99m HMPAO. In 8 patients, early scans with Tc-99m HMPAO tended to depict lesions more extensively when compared with CT. Early scanning with I-123 IMP was superior to that with Tc-99m HMPAO in detecting lesions. In detecting CCD, both methods were comparable to each other. In visualizing the basal ganglion, early scans with Tc-99m HMPAO were superior to scans with I-123 IMP. Tc-99m HMPAO has the advantages of short half-life period, small amounts of exposure doses, simple labeling, and prompt usage in emergency cases, such as cerebral infarction and cerebrovascular disorder. (Namekawa, K).

  6. MRI and N-isopropyl(I-123)p-iodoamphetamine SPECT findings in cases of moyamoya disease

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    Suto, Yuji; Kato, Terumi; Ohta, Yoshio (Tottori Univ., Yonago (Japan). School of Medicine); Caner, B.E.

    1993-07-01

    Six patients with moyamoya disease underwent magnetic resonance imaging (MRI), and in 4 of 6 cases, MRI findings were compared with N-isopropyl(I-123)p-iodoamphetamine (IMP) single-photon emission computed tomography (SPECT) brain scans to determine if there was a correlation between the morphology, as seen on MRI, and cerebral perfusion and/or metabolic changes detected by IMP-SPECT. MRI clearly revealed abnormalities secondary to moyamoya disease: nonvisualization of a signal void flow sign in the arteries, moyamoya vessels, and cerebral atrophy. All the perfusion abnormalities of the cortex, except a small one, observed on MR images were also detected on IMP-SPECT images, but small white matter abnormalities demonstrated on MR images could not be revealed by IMP-SPECT. Interestingly, in 2 patients, 2 additional cortical defects that were not observed on MR images were revealed by IMP-SPECT. Moreover, 3 areas with perfusion defects were larger on the IMP-SPECT scans than on the MR images. The cortical defects observed on the IMP-SPECT images but not on the MR images may reflect mild ischemia and/or certain metabolic abnormalities that lead to low tracer accumulation. Overall, IMP-SPECT and MRI may play complementary roles in the evaluation of this disease. (author).

  7. CT-Based Attenuation Correction in I-123-Ioflupane SPECT

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    Lange, Catharina; Seese, Anita; Schwarzenböck, Sarah; Steinhoff, Karen; Umland-Seidler, Bert; Krause, Bernd J.; Brenner, Winfried; Sabri, Osama

    2014-01-01

    Purpose Attenuation correction (AC) based on low-dose computed tomography (CT) could be more accurate in brain single-photon emission computed tomography (SPECT) than the widely used Chang method, and, therefore, has the potential to improve both semi-quantitative analysis and visual image interpretation. The present study evaluated CT-based AC for dopamine transporter SPECT with I-123-ioflupane. Materials and methods Sixty-two consecutive patients in whom I-123-ioflupane SPECT including low-dose CT had been performed were recruited retrospectively at 3 centres. For each patient, 3 different SPECT images were reconstructed: without AC, with Chang AC and with CT-based AC. Distribution volume ratio (DVR) images were obtained by scaling voxel intensities using the whole brain without striata as reference. For assessing the impact of AC on semi-quantitative analysis, specific-to-background ratios (SBR) in caudate and putamen were obtained by fully automated SPM8-based region of interest (ROI) analysis and tested for their diagnostic power using receiver-operator-characteristic (ROC) analysis. For assessing the impact of AC on visual image reading, screenshots of stereotactically normalized DVR images presented in randomized order were interpreted independently by two raters at each centre. Results CT-based AC resulted in intermediate SBRs about half way between no AC and Chang. Maximum area under the ROC curve was achieved by the putamen SBR, with negligible impact of AC (0.924, 0.935 and 0.938 for no, CT-based and Chang AC). Diagnostic accuracy of visual interpretation also did not depend on AC. Conclusions The impact of CT-based versus Chang AC on the interpretation of I-123-ioflupane SPECT is negligible. Therefore, CT-based AC cannot be recommended for routine use in clinical patient care, not least because of the additional radiation exposure. PMID:25268228

  8. CT-based attenuation correction in I-123-ioflupane SPECT.

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    Catharina Lange

    Full Text Available PURPOSE: Attenuation correction (AC based on low-dose computed tomography (CT could be more accurate in brain single-photon emission computed tomography (SPECT than the widely used Chang method, and, therefore, has the potential to improve both semi-quantitative analysis and visual image interpretation. The present study evaluated CT-based AC for dopamine transporter SPECT with I-123-ioflupane. MATERIALS AND METHODS: Sixty-two consecutive patients in whom I-123-ioflupane SPECT including low-dose CT had been performed were recruited retrospectively at 3 centres. For each patient, 3 different SPECT images were reconstructed: without AC, with Chang AC and with CT-based AC. Distribution volume ratio (DVR images were obtained by scaling voxel intensities using the whole brain without striata as reference. For assessing the impact of AC on semi-quantitative analysis, specific-to-background ratios (SBR in caudate and putamen were obtained by fully automated SPM8-based region of interest (ROI analysis and tested for their diagnostic power using receiver-operator-characteristic (ROC analysis. For assessing the impact of AC on visual image reading, screenshots of stereotactically normalized DVR images presented in randomized order were interpreted independently by two raters at each centre. RESULTS: CT-based AC resulted in intermediate SBRs about half way between no AC and Chang. Maximum area under the ROC curve was achieved by the putamen SBR, with negligible impact of AC (0.924, 0.935 and 0.938 for no, CT-based and Chang AC. Diagnostic accuracy of visual interpretation also did not depend on AC. CONCLUSIONS: The impact of CT-based versus Chang AC on the interpretation of I-123-ioflupane SPECT is negligible. Therefore, CT-based AC cannot be recommended for routine use in clinical patient care, not least because of the additional radiation exposure.

  9. Evaluation of SPECT with N-isopropyl (I-123)-p-iodoamphetamine (IMP) or technetium-99m ( sup 99m Tc)-d,1-hexamethyl-propyleneamine oxime (d,1-HM-PAO) in cerebral concussion patients

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    Torigoe, Ryuichiro; Hayashi, Takashi; Anegawa, Shigetaka (Saint Mary' s Hospital, Kurume, Fukuoka (Japan)); Harada, Katsuhiko; Matsuo, Hiromasa; Yoshikawa, Ichiro

    1991-06-01

    {sup 123}I-IMP and Tc-PAO SPECT were performed in 20 cases of cerebral concussion ranging in age from 4 to 20 years old, including six cases of the juvenile head trauma syndrome (JHTS). The SPECT findings were divided into two main types: six cases in the normal group with no blood flow abnormalities, and 14 cases in abnormal group showing reduced blood flow, mainly in cerebellum and occipital lobe except in one case. In 10 cases of reduced blood flow which could be analyzed, calculation of the blood flow ratio in the temporal and occipital lobes and the cerebellum with the frontal lobe taken as 100 showed values of 93.5% for the temporal lobe, 82.7% for the occipital lobe and 76.8% for the cerebellum. A statistically significant reduction in blood flow occurred in the occipital lobe and cerebellum. In blood examination, abnormally high values of white blood cell counts were observed transiently in 94% of cerebral concussion cases. Abnormalities in brain stem and hypothalamus appeared to cause these abnormal WBC values. From these findings, it was suggested that the blood flow regions of the basilar and posterior cerebral arteries, i.e., the brain stem and hypothalamus are closely connected with the lesions responsible for cerebral concussion. It also appeared that the JHTS occurs in cerebral concussion cases where recovery of the abnormal blood flow in these regions in poor. (author).

  10. Quantifying regional cerebral blood flow by N-isopropyl-P-[I-123]iodoamphetamine (IMP) using a ring type single-photon emission computed tomography system

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    Takahashi, N.; Odano, I.; Ohkubo, M. [Niigata Univ. (Japan)] [and others

    1994-05-01

    We developed a more accurate quantitative measurement of regional cerebral blood flow (rCBF) with the microsphere model using N-isopropyl-p-[I-123] iodoamphetamine (IMP) and a ring type single photon emission computed tomography (SPECT) system. SPECT studies were performed in 17 patients with brain diseases. A dose of 222 MBq (6 mCi) of [I-123]IMP was injected i.v., at the same time a 5 min period of arterial blood withdrawal was begun. SPECT data were acquired from 25 min to 60 min after tracer injection. For obtaining the brain activity concentration at 5 min after IMP injection, total brain counts collections and one minute period short time SPECT studies were performed at 5, 20, and 60 min. Measurement of the values of rCBF was calculated using short time SPECT images at 5 min (rCBF), static SPECT images corrected with total cerebral counts (rCBF{sub Ct}.) and those corrected with reconstructed counts on short time SPECT images (rCBF{sub Cb}). There was a good relationship (r=0.69) between rCBF and rCBF{sub Ct}, however, rCBF{sub Ct} tends to be underestimated in high flow areas and overestimated in low flow areas. There was better relationship between rCBF and rCBF{sub Cb}(r=0.92). The overestimation and underestimation shown in rCBF{sub Ct} was considered to be due to the correction of reconstructed counts using a total cerebral time activity curve, because of the kinetic behavior of [I-123]IMP was different in each region. We concluded that more accurate rCBF values could be obtained using the regional time activity curves.

  11. Iodine-123 iodobenzofuran (I-123 IBF) SPECT in patients with parkinsonism

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    Nakabeppu, Yoshiaki; Nakajo, Masayuki; Mitsuda, Mitsuru; Tsuchimochi, Shinsaku; Tani, Atsushi; Osame, Mitsuhiro [Kagoshima Univ. (Japan). Faculty of Medicine

    1999-12-01

    Iodine-123 benzofuran (I-123 IBF) is a dopaminergic antagonist which is suitable for SPECT imaging of D2 receptors. The purpose of this study is to evaluate the potential usefulness of semi-quantitative parameters obtained from brain SPECT data of I-123 IBF for differential diagnosis in patients with parkinsonism (PN). Subjects were 10 patients with PN: 2 patients with striato-nigral degeneration (SND), 5 patients with Parkinson's disease (PD), 2 patients with progressive supranuclear palsy (PSP) and one patient with olivo-ponto-cerebellar atrophy (OPCA). The data were acquired with a triple-head gamma camera at 2 hours after intravenous injection of 167 MBq of I-123 IBF. Transverse images were reconstructed by means of filtered backprojection, and attenuation correction was performed by Chang's method ({mu}=0.08). The basal ganglia-to-frontal cortex ratio (GFR) and the basal ganglia-to-occipital cortex ratio (GOR) on slices of 5 different thicknesses were calculated. The GFR and GOR were lower in the SND group than in the other disease groups in all slices with different thicknesses (7.2 mm, 14.4 mm, 21.6 mm, 28.8 mm and 43.2 mm). The semiquantitative parameters (GFR and GOR) obtained from brain SPECT data at 2 hours after intravenous injection of I-123 IBF may be useful for differential diagnosis in patients with PN. (author)

  12. Dopamine transporter density of the basal ganglia assessed with I-123 IPT SPECT in methamphetamine abusers

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    Lee, Joo Ryung; Ahn, Byeong Cheol [Kyungpook National University Medical School, Daegu (Korea, Republic of); Kewm, Do Hun [National Bugok Mental Hospital, Changryung (Korea, Republic of)] (and others)

    2005-10-15

    Functional imaging of dopamine transporter (DAT) defines integrity of the dopaminergic system, and DAT is the target site of drugs of abuse such as cocaine and methamphetamine. Functional imaging the DAT may be a sensitive and selective indicator of neurotoxic change by the drug. The aim of the present study is to evaluate the clinical implications of qualitative/quantitative analyses of dopamine transporter imaging in methamphetamine abusers. Six detoxified methamphetamine abusers (abuser group) and 4 volunteers (control group) were enrolled in this study. Brain MRI was performed in all of abuser group. Abuser group underwent psychiatric and depression assessment using brief psychiatric rating scale (BPRS) and Hamilton depression rating scale (HAMD), respectively. All of the subjects underwent I-123 IPT SPECT (IPT SPECT). IPT SPECT image was analysed with visual qualitative method and quantitative method using basal ganglia dopamine transporter (DAT) specific/non-specific binding ratio (SBR). Comparison of DAT SBR between abuser and control groups was performed. We also performed correlation tests between psychiatric and depression assessment results and DAT SBR in abuser group. All of abuser group showed normal MRI finding, but had residual psychiatric and depressive symptoms, and psychiatric and depressive symptom scores were exactly correlated (r=1.0, {rho} =0.005) each other. Five of them showed abnormal finding on qualitative visual I-123 IPT SPECT. Abuser group had lower basal ganglia DAT SBR than that of control (2.38 {+-} 0.20 vs 3.04 {+-} 0.27, {rho} =0.000). Psychiatric and depressive symptoms were negatively well correlated with basal ganglia DAT SBR (r=-0.908, {rho} =0.012, r=-0.924, {rho} =0.009) This results suggest that dopamine transporter imaging using I-123 IPT SPECT may be used to evaluate dopaminergic system of the basal ganglia and the clinical status in methamphetamine abusers.

  13. [sup 123]I-IMP SPECT studies in schizophrenia and atypical psychosis

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    Hayashi, Takuji; Suga, Hidemichi (Aichi Medical University, Nagakute (Japan))

    1993-05-01

    According to the classification of Mitsuda, 23 patients with endogenous psychosis aged 40 years or younger, presenting with hallucination and delusion, were classified as having schizophrenia (n=12) or atypical psychosis (n=11). These patients were studied by single photon emission computed tomography (SPECT) with N-isopropyl-p-I-123-iodoamphetamine (I-123 IMP). Sixteen healthy persons served as controls. Early and delayed SPECT images were obtained 30 min and 4 hr, respectively, after intravenous injection of I-123 IMP. The group of schizophrenic patients had markedly decreased uptake of I-123 in the basal ganglia, as well as the right temporal and left occipital areas on both early and delayed images. In the group of atypical psychosis patients, however, decreased uptake of I-123 was noted in both the right basal ganglia and left occipital area on early images, but none of such findings were seen on delayed images. Regarding the uptake ratio in the frontal area on both early and delayed images, there were significant differences between the two groups. These findings have important implications for the different etiology of both disease types: not only functional disturbance in the frontal area but also irreversible changes may be involved in the occurrence of schizophrenia, and functional disturbance particularly in the right basal ganglia may be involved in the occurrence of atypical psychosis. (N.K.).

  14. Iodine-123 IMP SPECT before and after by-pass surgery in a patient with occlusion of left anterior and middle cerebral arteries with basal abnormal telangiectasis (unilateral Moyamoya disease)

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    Honda, Norinari; Machida, Kikuo; Takishima, Teruo; Kaizu, Hiroyuki; Sugimoto, Eiichi

    1987-09-01

    A case of left anterior and middle cerebral arterial occlusion with angiographic features similar to Moyamoya disease was reported. IMP SPECT of the patient revealed the success of by-pass surgery clearly. The patient complained of transient right hemiparesis with aphasia 4 times. The cerebral arteriography disclosed occlusions of left anterior and middle cerebral arteries at their proximal portions. Right internal carotid and its branches were normal. I-123 IMP SPECT study showed hypoperfusion in left temporal lobe, basal ganglia with incomplete reperfusion on the delayed (4 hours after injection) SPECT images. After the superficial temporal-middle cerebral artery anastomosis, I-123 IMP SPECT showed improvement of the brain blood flow. I-123 IMP SPECT was very useful in detecting the ischemic areas and evaluating the revascularizing surgery in this case.

  15. Reliability evaluation of I-123 ADAM SPECT imaging using SPM software and AAL ROI methods

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    Yang, Bang-Hung; Tsai, Sung-Yi; Wang, Shyh-Jen; Su, Tung-Ping; Chou, Yuan-Hwa; Chen, Chia-Chieh; Chen, Jyh-Cheng

    2011-08-01

    The level of serotonin was regulated by serotonin transporter (SERT), which is a decisive protein in regulation of serotonin neurotransmission system. Many psychiatric disorders and therapies were also related to concentration of cerebral serotonin. I-123 ADAM was the novel radiopharmaceutical to image SERT in brain. The aim of this study was to measure reliability of SERT densities of healthy volunteers by automated anatomical labeling (AAL) method. Furthermore, we also used statistic parametric mapping (SPM) on a voxel by voxel analysis to find difference of cortex between test and retest of I-123 ADAM single photon emission computed tomography (SPECT) images.Twenty-one healthy volunteers were scanned twice with SPECT at 4 h after intravenous administration of 185 MBq of 123I-ADAM. The image matrix size was 128×128 and pixel size was 3.9 mm. All images were obtained through filtered back-projection (FBP) reconstruction algorithm. Region of interest (ROI) definition was performed based on the AAL brain template in PMOD version 2.95 software package. ROI demarcations were placed on midbrain, pons, striatum, and cerebellum. All images were spatially normalized to the SPECT MNI (Montreal Neurological Institute) templates supplied with SPM2. And each image was transformed into standard stereotactic space, which was matched to the Talairach and Tournoux atlas. Then differences across scans were statistically estimated on a voxel by voxel analysis using paired t-test (population main effect: 2 cond's, 1 scan/cond.), which was applied to compare concentration of SERT between the test and retest cerebral scans.The average of specific uptake ratio (SUR: target/cerebellum-1) of 123I-ADAM binding to SERT in midbrain was 1.78±0.27, pons was 1.21±0.53, and striatum was 0.79±0.13. The cronbach's α of intra-class correlation coefficient (ICC) was 0.92. Besides, there was also no significant statistical finding in cerebral area using SPM2 analysis. This finding might help us

  16. Reliability evaluation of I-123 ADAM SPECT imaging using SPM software and AAL ROI methods

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    Yang, Bang-Hung [Department of Biomedical Imaging and Radiological Sciences, National Yang-Ming University, Taipei, Taiwan (China); Department of Nuclear Medicine, Taipei Veterans General Hospital, Taiwan (China); Tsai, Sung-Yi [Department of Biomedical Imaging and Radiological Sciences, National Yang-Ming University, Taipei, Taiwan (China); Department of Imaging Medical, St.Martin De Porres Hospital, Chia-Yi, Taiwan (China); Wang, Shyh-Jen [Department of Biomedical Imaging and Radiological Sciences, National Yang-Ming University, Taipei, Taiwan (China); Department of Nuclear Medicine, Taipei Veterans General Hospital, Taiwan (China); Su, Tung-Ping; Chou, Yuan-Hwa [Department of Psychiatry, Taipei Veterans General Hospital, Taipei, Taiwan (China); Chen, Chia-Chieh [Institute of Nuclear Energy Research, Longtan, Taiwan (China); Chen, Jyh-Cheng, E-mail: jcchen@ym.edu.tw [Department of Biomedical Imaging and Radiological Sciences, National Yang-Ming University, Taipei, Taiwan (China)

    2011-08-21

    The level of serotonin was regulated by serotonin transporter (SERT), which is a decisive protein in regulation of serotonin neurotransmission system. Many psychiatric disorders and therapies were also related to concentration of cerebral serotonin. I-123 ADAM was the novel radiopharmaceutical to image SERT in brain. The aim of this study was to measure reliability of SERT densities of healthy volunteers by automated anatomical labeling (AAL) method. Furthermore, we also used statistic parametric mapping (SPM) on a voxel by voxel analysis to find difference of cortex between test and retest of I-123 ADAM single photon emission computed tomography (SPECT) images. Twenty-one healthy volunteers were scanned twice with SPECT at 4 h after intravenous administration of 185 MBq of {sup 123}I-ADAM. The image matrix size was 128x128 and pixel size was 3.9 mm. All images were obtained through filtered back-projection (FBP) reconstruction algorithm. Region of interest (ROI) definition was performed based on the AAL brain template in PMOD version 2.95 software package. ROI demarcations were placed on midbrain, pons, striatum, and cerebellum. All images were spatially normalized to the SPECT MNI (Montreal Neurological Institute) templates supplied with SPM2. And each image was transformed into standard stereotactic space, which was matched to the Talairach and Tournoux atlas. Then differences across scans were statistically estimated on a voxel by voxel analysis using paired t-test (population main effect: 2 cond's, 1 scan/cond.), which was applied to compare concentration of SERT between the test and retest cerebral scans. The average of specific uptake ratio (SUR: target/cerebellum-1) of {sup 123}I-ADAM binding to SERT in midbrain was 1.78{+-}0.27, pons was 1.21{+-}0.53, and striatum was 0.79{+-}0.13. The cronbach's {alpha} of intra-class correlation coefficient (ICC) was 0.92. Besides, there was also no significant statistical finding in cerebral area using SPM2

  17. Serotonin transporters in Parkinson's disease studied with [I-123]{beta}-CIT SPECT

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    Kim, Sang Eun; Choi, Joon Young; Choe, Yearn Seong; Lee, Won Yong; Choi, Yong; Lee, Kyung Han; Kim, Byung Tae [Samsung Medical Center, Seoul (Korea, Republic of)

    1997-07-01

    In Parkinson's disease (PD), both neuropathological and biochemical studies suggest that serotonin (5-HT) neurons are affected by the disease process. The purpose of this study was to assess the integrity of 5-HT transporters in PD using [I-123]{beta}-CIT (2{beta}-carbomethoxy-3{beta}-(4-iodophenyl)tropane], which binds to both 5-HT and dopamine transporters, and SPECT. Forty-five patients with PD at relatively early stages (Hoehn-Yahr stages I-3) and seven agematched healthy controls were injected with 185-370 MBq(I-123]{beta}-CIT. A total of 15 SPECT scans were acquired over a 24 hr period in each subject using a three-headed system. Time activity curves were obtained in the hypothalamic/midbrain region, where the binding of [I-123] {beta}-CIT is associated primarily with 5-HT transporters, and cerebellum, which was assumed to include only nondisplaceable activity. The parameter k3/k4 (forward/dissociation rate), which is a measure of 5-HT transporter density, was estimated by four noninvasive methods: peak equilibrium ratio (PER) [the ratio of specific binding (hypothalamic/midbrain region-cerebellar activity) to nondisplaceable (cerebella) activity when specific binding reaches a peak], area ratio (the ratio of the areas under the specific binding and nondisplaceable activity curves), k3/k4 obtained using a kinetic two-compartment model [R(2C)], and k3/k4 from a modified graphic method [R(DV)] (Ichise et al., 1996). In the healthy controls, the activity in the hypothalamic/midbrain area generally peaked by 3 hr after tracer injection, then decreased slowly; similar patterns were observed in the PD patients. The k3/k4 values estimated by four different methods in the PD patients did not differ significanlty from those in the healthy controls. In the PD patients, the k3/k4 estimates were not significantly correlated with either Hoehn-Yahr scale or UPDRS (Unified Parkinson's Disease Rating Scale) scores, including total, motor, activities of daily living

  18. Pathophysiologic study of chronic infarcts with I-123 isopropyl iodo-amphetamine (IMP)

    DEFF Research Database (Denmark)

    Raynaud, C; Rancurel, G; Samson, Y;

    1987-01-01

    in IMP uptake and rCBF averaging 34% and 46% respectively and by a hypodense image on the x-ray computerized tomography scan. The second, the periinfarct or "peripheral area" was characterized by a moderate decrease in IMP uptake and regional cerebral blood flow averaging 13 and 19% respectively......; this area extended around the central area and had a normal density on computerized tomography scan. The IMP hypofixation of the peripheral area observed at the 10th minute tended to disappear at the 5th hour. The volume of this area was often found to be quite large, covering more than 30% of a hemisphere...

  19. Comparison of I-123 MIBG planar imaging and SPECT for the detection of decreased heart uptake in Parkinson disease.

    Science.gov (United States)

    Oh, Jin-Kyoung; Choi, Eun-Kyoung; Song, In-Uk; Kim, Joong-Seok; Chung, Yong-An

    2015-10-01

    Decreased myocardial uptake of I-123 metaiodobenzylguanidine (MIBG) is an important finding for diagnosis of Parkinson's disease (PD). This study compared I-123 MIBG SPECT and planar imaging with regard to their diagnostic yield for PD. 52 clinically diagnosed PD patients who also had decreased striatal uptake on FP-CIT PET/CT were enrolled. 16 normal controls were also included. All underwent cardiac MIBG planar scintigraphy and SPECT separately. Myocardial I-123 MIBG uptake was interpreted on planar and SPECT/CT images separately by visual and quantitative analysis. The final diagnosis was made by consensus between two readers. Kappa analyses were performed to determine inter-observer agreement for both methods. Sensitivity, specificity, and accuracy were compared with McNemar's test. The sensitivity, specificity, and accuracy were 84.6, 100, and 88.2% for planar images and 96.2, 100 and 97.1% for SPECT, respectively, with a significant difference between the two imaging methods (p diagnostic performance for PD than planar images. Increased lung uptake may cause false-negative results on planar imaging.

  20. Dopamine transporter density of the basal ganglia in children with attention deficit hyperactivity disorder assessed with I-123 IPT SPECT

    Energy Technology Data Exchange (ETDEWEB)

    Ryu, Won Gee; Kim, Tae Hoon; Ryu, Young Hoon; Yun, Mi Jin; Lee, Jong Doo; Cheon, Keun Ah [College of Medicine, Yonsei Univ., Seoul (Korea, Republic of); Chi, Dae Yoon [College of Medicine, Inha Univ., Incheon (Korea, Republic of); Kim, Jong Ho; Choi, Tae Hyun [School of Medicine, Gachon Univ., Gachon (Korea, Republic of)

    2003-08-01

    Attention deficit hyperactivity disorder (ADHD) has been known as psychiatric disorder in childhood associated with dopamine dysregulation. In present study, we investigated changes in dopamine transporter (DAT) density of the basal ganglias using I-123 N-(3-iodopropen-2-yl) -2-carbomethoxy-3beta-(4-chlorphenyl) tropane (I-123 IPT) SPECT in children with ADHD before and after methylphenidate treatment. Nine drug-naive children with ADHD and seven normal children were included in the study. We performed brain SPECT two hours after the intravenous administration of I-123 IPT and made both quantitative and qualitative analyses using the obtained SPECT data, which were reconstructed for the assessment of specific/nonspecific DAT binding ratios in the basal ganglia. All children with ADHD reperformed (123I)IPT SPECT after treatment with methylphenidate (0.7mg/kg/d) during about 8 weeks. SPECT data reconstructed for the assessment of specific/nonspecific DAT binding ratio of the basal ganglia were compared between before and after treatment methyphenidate. We investigated correlation between the change of ADHD symptom severity assessed with ADHD rating scale-IV and specific/nonspecific DAT binding ratio of basal ganglia. Children with ADHD had a significantly greater specific/nonspecific DAT binding ratio of the basal ganglia comparing to normal children (Right : z = 2.057, p = 0.041 ; Left : z = 2.096, p = 0.032). Under treatment with methylphenidate in all children with ADHD, specific/nonspecific DAT binding ratio of both ganglia decreased significantly greater than before treatment with methylphenidate (Right : t = 3.239, p = 0.018 ; Left : t = 3.133, p 0.020). However, no significant correlation between the change of ADHD symptom severity scores and specific/nonspecific DAT binding ratio of the basal ganglia were found. These findings support the complex dysregulation of the dopaminergic neurotransmitter system in children with ADHD.

  1. Differentiation of Parkinson's Disease and Essential Tremor on I-123 IPT(I-123-N-(3-iodopropen-2-yl)-2{beta}-carbomethoxy-3{beta}-(4-cholorophenyl) tropane) Brain SPECT

    Energy Technology Data Exchange (ETDEWEB)

    Pai, Moon Sun [Kwandong University College of Medicine, Koyang (Korea, Republic of); Choi, Tae Hyun [Korea Institute of Radiological and Medical Science, Seoul (Korea, Republic of); Ahn, Sung Min [Gachon University of Medicine and Science, Inchon (Korea, Republic of); Choi, Jai Yong; Ryu, Won Gee; Lee, Jae Hoon; Ryu, Young Hoon [Yonsei University College of Medicine, Seoul (Korea, Republic of)

    2009-04-15

    The study was to assess I-123-N-(3-iodopropen-2-yl)-2[beta]-carbomethoxy-3[beta]-(4-cholorophenyl) tropane (IPT) SPECT in differential diagnosis among early stage of Parkinson's disease(PD) and essential tremor(ET) and normal control(NL) groups quantitatively. I-123 IPT brain SPECT of 50 NL, 20 early PD, 30 advanced PD, and 20 ET were performed at 20 minutes and 2 hours. Specific/nonspecific binding of striatum was calculated by using right and left striatal specific to occipital non-specific uptake ratio (striatum-OCC/OCC). Mean value of specific/nonspecific binding ratio was significantly different between advanced PD group and NL group. However, significant overlap of striatal specific/nonspecific binding ratio was observed between PD group and ET group. Bilateral striatal specific/nonspecific binding ratios were decreased in advanced PD. Lateralized differences in the striatal uptake of I-123 IPT correlated with asymmetry in clinical findings in PD group. I-123 IPT SPECT may be a useful method for the diagnosis of PD and objective evaluation of progress of clinical stages. Care should be made in the differential diagnosis of early stage of PD and other motor disturbances mimicking PD such as ET in view of significant overlap in striatal I-123 specific/nonspecific binding ratio.

  2. Hemimegalencephaly: Clinical, EEG, neuroimaging, and IMP-SPECT correlation

    Energy Technology Data Exchange (ETDEWEB)

    Konkol, R.J.; Maister, B.H.; Wells, R.G.; Sty, J.R. (Medical College of Wisconsin, Milwaukee (USA))

    1990-11-01

    Iofetamine-single photon emission computed tomography (IMP-SPECT) was performed on 2 girls (5 1/2 and 6 years of age) with histories of intractable seizures, developmental delay, and unilateral hemiparesis secondary to hemimegalencephaly. Electroencephalography (EEG) revealed frequent focal discharges in 1 patient, while a nearly continuous burst suppression pattern over the malformed hemisphere was recorded in the other. IMP-SPECT demonstrated a good correlation with neuroimaging studies. In spite of the different EEG patterns, which had been proposed to predict contrasting clinical outcomes, both IMP-SPECT scans disclosed a similar decrease in tracer uptake in the malformed hemisphere. These results are consistent with the pattern of decreased tracer uptake found in other interictal studies of focal seizures without cerebral malformations. In view of recent recommendations for hemispherectomy in these patients, we suggest that the IMP-SPECT scan be used to compliment EEG as a method to define the extent of abnormality which may be more relevant to long-term prognosis than EEG alone.

  3. Ectopic Thyroid Tissue in the Mediastinum Characterized by Histology and Functional Imaging with I-123 SPECT/CT

    Science.gov (United States)

    Hummel, Jed; Wachsmann, Jason; Carrick, Kelley; Oz, Orhan K.; Mathews, Dana

    2017-01-01

    Ectopic thyroid tissue is a rare entity and when discovered it is typically along the pathway of embryologic migration of the thyroid. We present a case of incidental finding of ectopic thyroid tissue within mediastinum in a 61-year-old female patient with a history of total thyroidectomy for thyroiditis and nodules. The patient presented to emergency room with cough and right chest pain and underwent a chest computed tomographic angiogram (CTA) to exclude pulmonary embolism as part of chest pain workup. One right paratracheal mediastinal soft tissue nodule was visualized on the images of CTA. This right paratracheal soft tissue mass was found to be ectopic benign thyroid tissue by histological analysis of the biopsied tissue samples. The function of this ectopic thyroid tissue was characterized by I-123 radioiodine uptake and single photon emission computed tomography/computed tomography (SPECT/CT) imaging. This case illustrates that ectopic thyroid tissue should be included for differential diagnosis of a hyperdense soft tissue mass located within mediastinum. I-123 SPECT/CT is useful for guiding tissue biopsy of ectopic thyroid tissue distant from orthotopic thyroid gland and functional and anatomic characterization of mediastinal ectopic thyroid tissue for surgical resection when it is medically necessary. PMID:28251012

  4. Ectopic Thyroid Tissue in the Mediastinum Characterized by Histology and Functional Imaging with I-123 SPECT/CT.

    Science.gov (United States)

    Hummel, Jed; Wachsmann, Jason; Carrick, Kelley; Oz, Orhan K; Mathews, Dana; Peng, Fangyu

    2017-01-01

    Ectopic thyroid tissue is a rare entity and when discovered it is typically along the pathway of embryologic migration of the thyroid. We present a case of incidental finding of ectopic thyroid tissue within mediastinum in a 61-year-old female patient with a history of total thyroidectomy for thyroiditis and nodules. The patient presented to emergency room with cough and right chest pain and underwent a chest computed tomographic angiogram (CTA) to exclude pulmonary embolism as part of chest pain workup. One right paratracheal mediastinal soft tissue nodule was visualized on the images of CTA. This right paratracheal soft tissue mass was found to be ectopic benign thyroid tissue by histological analysis of the biopsied tissue samples. The function of this ectopic thyroid tissue was characterized by I-123 radioiodine uptake and single photon emission computed tomography/computed tomography (SPECT/CT) imaging. This case illustrates that ectopic thyroid tissue should be included for differential diagnosis of a hyperdense soft tissue mass located within mediastinum. I-123 SPECT/CT is useful for guiding tissue biopsy of ectopic thyroid tissue distant from orthotopic thyroid gland and functional and anatomic characterization of mediastinal ectopic thyroid tissue for surgical resection when it is medically necessary.

  5. Ectopic Thyroid Tissue in the Mediastinum Characterized by Histology and Functional Imaging with I-123 SPECT/CT

    Directory of Open Access Journals (Sweden)

    Jed Hummel

    2017-01-01

    Full Text Available Ectopic thyroid tissue is a rare entity and when discovered it is typically along the pathway of embryologic migration of the thyroid. We present a case of incidental finding of ectopic thyroid tissue within mediastinum in a 61-year-old female patient with a history of total thyroidectomy for thyroiditis and nodules. The patient presented to emergency room with cough and right chest pain and underwent a chest computed tomographic angiogram (CTA to exclude pulmonary embolism as part of chest pain workup. One right paratracheal mediastinal soft tissue nodule was visualized on the images of CTA. This right paratracheal soft tissue mass was found to be ectopic benign thyroid tissue by histological analysis of the biopsied tissue samples. The function of this ectopic thyroid tissue was characterized by I-123 radioiodine uptake and single photon emission computed tomography/computed tomography (SPECT/CT imaging. This case illustrates that ectopic thyroid tissue should be included for differential diagnosis of a hyperdense soft tissue mass located within mediastinum. I-123 SPECT/CT is useful for guiding tissue biopsy of ectopic thyroid tissue distant from orthotopic thyroid gland and functional and anatomic characterization of mediastinal ectopic thyroid tissue for surgical resection when it is medically necessary.

  6. The utility of segmental analysis in cardiac I-123 MIBG SPECT in Parkinson's disease

    Energy Technology Data Exchange (ETDEWEB)

    Kwon, Soo Hyun; Yoon, Joon Kee; Yoon, Jung Han; Lee, Su Jin; Jo, Kyung Soo; Lee, Dong Hyun; An, Young Sil [Ajou University School of Medicine, Suwon (Korea, Republic of)

    2015-12-15

    Cardiac images using I-123 metaiodobenzylguanidine (MIBG) are widely used to evaluate cardiac sympathetic denervation in Parkinson’s disease (PD). The aim of this study was to evaluate the utility of segmental analysis on cardiac MIBG SPECT in PD patients. In total, 36 patients with PD (n = 26) or essential tremor (ET, n = 10) who underwent MIBG cardiac SPECT were enrolled. The heart-to-mediastinum (H/M) ratios of MIBG uptake were acquired on planar images. For the segmental analysis of SPECT images, we evaluated the summed defect score (SDS) using a 17-segment model. The diagnostic abilities of H/M ratios and segmental parameters on MIBG SPECT were assessed by ROC curve analysis. The H/M ratios were significantly lower in PD than in ET patients (p < 0.05). On segmental analysis, SDS was significantly higher in PD patients than in the ET group (7.04 ± 4.09 vs. 2.90 ± 2.80; p = 0.006). The defect score of the anteroseptal region showed a significant difference between the groups (p = 0.002). The ROC analysis suggested only SDS (AUC = 0.785, p = 0.0003) and defect scores in the anteroseptal (AUC = 0.800, p < 0.0001) and inferior (AUC = 0.667, p = 0.013) regions showed significant diagnostic ability to differentiate PD from ET. Segmental parameters from cardiac MIBG SPECT images can provide additional information to differentiate PD from ET patients. Beyond H/M ratios from planar images, we recommend an MIBG SPECT study to evaluate sympathetic denervation in PD.

  7. Diagnosis of myocardial involvement in patients with systemic myopathies with 15-(p-[I-123]iodophenyl) pentadecanoic acid (IPPA) SPECT

    Energy Technology Data Exchange (ETDEWEB)

    Kropp, J.; Briele, B.; Smekal, A.V.; Hotze, A.L.; Biersack, H.J.; Koehler, U.; Zierz, St. [Bonn Univ. (Germany); Knapp, F.F. [Oak Ridge National Lab., TN (United States)

    1992-03-01

    Involvement of the myocardium in non-infectious myopathies presents in most cases as systolic dysfunction or a disturbed cardiac rhythm. We are interested in exploring how often cardiac involvement can be evaluated with various diagnostic techniques in patients with proven myopathy. We investigated 41 patients with myopathies of various etiology, including mitochondrial and congenital myopathies, Curshmann-Steinert disease, muscular dystrophy, and others. Myopathy was proven by muscular biopsy usually from the bicep. Fatty acid imaging was performed with 15-(p-[I-123]iodophenyl)pentadecanoic acid (IP-PA) and sequential SPECT-scintigraphy with a 180 deg. rotation starting at the 45 deg. RAO position. 190 MBq were injected at the maximal stage of a submaximal exercise. Filtered backprojection and reorientation of the slices were achieved by standard techniques. The quantitative comparison of the oblique slices (bulls-eye technique) of the SPECT-studies revealed turnover-rates as a qualitative measure of {beta}-oxidation. Serum levels of lactate (L), pyruvate (P), glucose (G) and triglycerides (TG) were measured at rest and stress. Ventricular function was investigated by radionuclide ventriculography (MUGA) at rest and under stress with Tc-99m labeled red blood cells. In addition, ECG, 24 hour-ECG, and echocardiography were also performed with standard techniques.

  8. Diagnosis of myocardial involvement in patients with systemic myopathies with 15-(p-(I-123)iodophenyl) pentadecanoic acid (IPPA) SPECT

    Energy Technology Data Exchange (ETDEWEB)

    Kropp, J.; Briele, B.; Smekal, A.V.; Hotze, A.L.; Biersack, H.J.; Koehler, U.; Zierz, St. (Bonn Univ. (Germany)); Knapp, F.F. (Oak Ridge National Lab., TN (United States))

    1992-01-01

    Involvement of the myocardium in non-infectious myopathies presents in most cases as systolic dysfunction or a disturbed cardiac rhythm. We are interested in exploring how often cardiac involvement can be evaluated with various diagnostic techniques in patients with proven myopathy. We investigated 41 patients with myopathies of various etiology, including mitochondrial and congenital myopathies, Curshmann-Steinert disease, muscular dystrophy, and others. Myopathy was proven by muscular biopsy usually from the bicep. Fatty acid imaging was performed with 15-(p-(I-123)iodophenyl)pentadecanoic acid (IP-PA) and sequential SPECT-scintigraphy with a 180 deg. rotation starting at the 45 deg. RAO position. 190 MBq were injected at the maximal stage of a submaximal exercise. Filtered backprojection and reorientation of the slices were achieved by standard techniques. The quantitative comparison of the oblique slices (bulls-eye technique) of the SPECT-studies revealed turnover-rates as a qualitative measure of {beta}-oxidation. Serum levels of lactate (L), pyruvate (P), glucose (G) and triglycerides (TG) were measured at rest and stress. Ventricular function was investigated by radionuclide ventriculography (MUGA) at rest and under stress with Tc-99m labeled red blood cells. In addition, ECG, 24 hour-ECG, and echocardiography were also performed with standard techniques.

  9. Experimental determination of the weighting factor for the energy window subtraction-based downscatter correction for I-123 in brain SPECT studies

    DEFF Research Database (Denmark)

    de Nijs, Robin; Holm, Søren; Thomsen, Gerda;

    2010-01-01

    Correction for downscatter in I-123 SPECT can be performed by the subtraction of a secondary energy window from the main window, as in the triple-energy window method. This is potentially noise sensitive. For studies with limited amount of counts (e.g. dynamic studies), a broad subtraction window...

  10. Dopamine Transporter Density of the Basal Ganglia Assessed with I-123 IPT SPECT in Patients with Obsessive-Compulsive Disorder

    Energy Technology Data Exchange (ETDEWEB)

    Ryu, W. K.; Ryu, Y. H.; Yoon, M. J.; Kim, C. H.; Chun, K. A.; Lee, J. D. [College of Medicine, Univ. of Yonsei, Seoul (Korea, Republic of); Jee, D. Y. [College of Medicine, Univ. of Inhwa, Seoul (Korea, Republic of); Choi, T. H. [Korea Cancer Center Hospital, Seoul (Korea, Republic of)

    2003-07-01

    It has been suggested that dopamine as well as serotonin is associated with the pathophysiology of obsessive-compulsive disorder (OCD). Thus, many studies about brain regions associated with dopamine in OCD have been performed. In the present study, we investigated the DAT density of the basal ganglia using iodine-123 labelled N-(3-iodopropen-2-yl) - 2beta - carbomethoxy - 3beta - (4 - chloropheny1) tropane (I-123 IPT) single-photon emission tomography (SPECT) in patients with OCD and evaluated the activity of the presynaptic dopamine function in patients with OCD. Fifteen patients with OCD and nineteen normal control adults were included in the study. We performed brain SPET 2 hours after the intravenous administration of I-123 IPT and carried out both quantitative and qualitative analyses using the obtained SPET data, which were reconstructed for the assessment of the specific/non-specific DAT binding ratio in the basal ganglia. We then investigated the correlation between the severity scores of OCD symptoms assessed with the Yale-Brown Obsessive-Compulsive Scale (Y-BOCS) and the specific/non-specific DAT binding ratio of the basal ganglia. Patients with OCD showed a significantly increased specific/non-specific DAT binding ratio in right basal ganglia compared with normal control adults and an increased tendency in the specific/non-specific DAT binding ratio in left basal ganglia. No significant correlation was found between the total scores of the Y-BOCS and the specific/non-specific DAT binding ratio of the basal ganglia. Our findings suggest that the dopaminergic neurotransmitter system of the basal ganglia in patients with OCD plays an important role in fronto-subcortical circuit well-known as the pathophysiological mechanism of OCD.

  11. Sequential sup 123 I-IMP-SPECT in acute infantile hemiplegia

    Energy Technology Data Exchange (ETDEWEB)

    Shirasaka, Y.; Ito, M.; Okuno, T.; Fujii, T.; Mikawa, H. (Kyoto Univ. (Japan))

    1989-09-01

    Sequential {sup 123}I-N-isopropyl-p-iodoamphetamine (IMP) single-photon emission computed tomography (SPECT) was performed in 2 patients with acute infantile hemiplegia. In both patients, low uptake of IMP was detected in the targeted abnormal hemisphere. The {sup 123}I-IMP-SPECT findings indicative of a pathologic condition persisted even when the clinical findings and electroencephalographic abnormalities improved. Because of its sensitivity, noninvasiveness, and accurate reflection of the cerebral blood flow distribution, {sup 123}I-IMP-SPECT is useful in the examination of acute infantile hemiplegia and in the evaluation of prognosis.

  12. sup 123 I-IMP-SPECT in vascular dementia

    Energy Technology Data Exchange (ETDEWEB)

    Namura, Yasuhiro; Miyoshi, Toshihiko; Shio, Hideo; Yamaguchi, Shinya; Kimura, Jun (Kyoto Univ. (Japan). Hospital)

    1990-12-01

    Cerebral blood flow (CBF) was studied by N-isopropyl-p-{sup 123}I-iodoamphetamine (IMP)-single photon emission computed tomography (SPECT) in patients with vascular dementia. Patients were divided by CT/MRI findings into multiple or localized type and cortical or subcortical type. Of patients with multiple lesions in the cortex, the reduction of CBF was noted in several cortex corresponding to CT/MRI findings. Patients in this group had a high incidence of aphasia, agraphia, dyscalculia, and amnesia. Patients with multiple lesions in the white matter and basal ganglia were subdivided into 3 groups by clinical manifestations. CBF in those with dementia showed generalized reduction in all cerebral cortex and especially in bilateral frontal regions. CBF in those with slightly impaired mentality was almost normal. In another group with pseudobulbar palsy and/or extrapyramidal sings, CBF was reduced intermediately only in bilateral frontal regions. Typical symptoms in demented patients were apathy, emotional lability, and forgetfulness. In those with the localized type, CBF was reduced in cerebral cortical areas correlating with the impaired mental functions. (author).

  13. A comparison of different energy window subtraction methods to correct for scatter and downscatter in I-123 SPECT imaging

    DEFF Research Database (Denmark)

    Lagerburg, Vera; de Nijs, Robin; Holm, Søren

    2012-01-01

    One of the main problems in quantification of single photon emission computer tomography imaging is scatter. In iodine-123 (I-123) imaging, both the primary 159 keV photons and photons of higher energies are scattered. In this experimental study, different scatter correction methods, based on ene...... on energy window subtraction, have been compared with each other....

  14. Experimental determination of the weighting factor for the energy window subtraction-based downscatter correction for I-123 in brain SPECT studies

    DEFF Research Database (Denmark)

    de Nijs, Robin; Holm, Søren; Thomsen, Gerda;

    2010-01-01

    were investigated in this study. Energy windows with a width of 32 keV were centered at 159 keV and 200 keV. The weighting factor was measured both with an I-123 point source and in a dopamine transporter brain SPECT study in 10 human subjects (5 healthy subjects and 5 patients) by minimizing......Correction for downscatter in I-123 SPECT can be performed by the subtraction of a secondary energy window from the main window, as in the triple-energy window method. This is potentially noise sensitive. For studies with limited amount of counts (e.g. dynamic studies), a broad subtraction window...... with identical width is preferred. This secondary window needs to be weighted with a factor higher than one, due to a broad backscatter peak from high-energy photons appearing at 172 keV. Spatial dependency and the numerical value of this weighting factor and the image contrast improvement of this correction...

  15. {alpha}4 {beta}2 nicotinic acetylcholine receptor in Alzheimer's disease and mild cognitive impairment: a study with 5-[I-123]iodo-A-85380 SPECT

    Energy Technology Data Exchange (ETDEWEB)

    Park, Eun Kyung; Kim, Yu Kyeong; Kim, Sang Yun; Kim, Sang Eun [Seoul National Univ. College of Medicine, Seoul (Korea, Republic of)

    2007-07-01

    It has been reported that the number of neuronal nicotinic acetylcholine receptors (nAChRs) is decreased in Alzheimer's disease (AD) and the degree of cholinergic deficit is correlated with cognitive impairment. We examined neuronal nAChR distribution of AD patients using 5-[I-123]iodo-A85380 (5-IA) SPECT and correlated it with the pattern of cerebral glucose metabolic impairment and the severity of cognitive impairment. Five clinically diagnosed AD patients, 5 amnestic mild cognitive impairment (MCI) patients, and 10 age- and sex-matched healthy controls were studied with 5-IA SPECT and brain FDG PET. 5-IA SPECT was performed for 30 min at 120 min after radiotracer injection. FDG PET was done within one month interval. Neuropsychological tests were done for cognitive evaluation. A nAChR parameter DV was calculated in brain regions using cerebellum as reference tissue. All scan images were analyzed using SPM2 and ANOVA was done for group comparison. P value less than 0.005 was considered significant. 5-IA SPECT images of AD patients revealed significantly reduced nAChR distribution in the anterior cingulate cortex, striatum, and the left temporal cortex. MCI patients demonstrated decreased receptor distribution mainly in the subcortical areas. Cortical nAChR distribution showed correlation with cortical glucose metabolism and subcortical with that of posterior cingulate cortex (PCC). Episodic memory and semantic verbal fluency showed significant correlation with nAChR distribution of periventricular white matter (PVWM), visuospatial function evaluated with RCFT with that of PCC, left temporoparietal cortex, and frontal lobe white matter, and MMSE with that of PVWM, frontal cortex, and striatum. These data demonstrate reduction of nAChR distribution in patients with AD, which has significant correlation with cerebral glucose metabolism and cognitive impairment. It might be useful for diagnosis of AD, and for monitoring individualized treatments targeted at nAChRs.

  16. Regional cerebral blood flow in various pediatric neurological patients using /sup 123/I-IMP SPECT

    Energy Technology Data Exchange (ETDEWEB)

    Konishi, Tohru; Naganuma, Yoshihiro; Hongou, Kazuhisa; Murakami, Miyako; Yamatani, Miwa; Okada, Toshio

    1988-03-01

    The recent development of a new radiopharmaceutical /sup 123/I-isopropyl-iodoamphetamine (IMP), which is taken up by the brain from the blood flow, has offered a possibility of constructing scintigraphy maps of regional cerebral blood flow (rCBF) using single photon emission CT. We used this mehtod in various pediatric neurological diseases. Six patients with cerebro-vascular disorders (moya-moya disease 2, infarction 3 and HHE syndrome 1), 6 patients with infectious diseases of CNS (acute encephalitis 4, septic meningitis 1 and SSPE 1) and a miscellaneous group of six patients were studied. The rCBF abnormalities in cerebro-vascular diseases were more extensive and frequent than x-ray CT abnormalities. Repeated studies of IMP-SPECT revealed usefulness for the understanding of changeable hemodynamic pathophysiology and for the judgment of theraptic effectiveness and prognosis. The rCBF decrease in infectious diseases tended to be more diffuse and slight than that in cerebro-vascular diseases. In almost all patients, the area of rCBF decrease coincided with the area of EEG slowing evaluated by EEG topographic analysis. Brain imaging using /sup 123/I-IMP SPECT may reveal functional abnormalities as well as organic lesions. /sup 123/I-IMP SPECT has introduced a new era for the useful application of nuclear medicine to the investigation of pediatric neurological diseases.

  17. Comparison of two I-123 labeled SPECT probes, for the dopamine transporter in non-human primate brain

    Energy Technology Data Exchange (ETDEWEB)

    Gandelman, M.S.; Scanley, B.E.; Al-Tikrite, M.S. [Yale Univ., West Haven, CT (United States)] [and others

    1994-05-01

    A comparative SPECT evaluation of the regional uptake of 28-carboisopropoxy-3{beta}-(4-iodophenyl)tropane (IP-CIT) and 2{beta}-carbomethoxy-3{beta}-(4-iodophenyl)tropane ({beta}-CIT) was performed to assess the improved specificity of IP-CIT over {beta}-CIT for the dopamine (DE) transporter, as shown previously by in vitro studies (n=10), ranging from 7 to 10 hours with 6.9 to 15 mCi injected dose, were completed in 3 baboons. Peripheral metabolism of the two ligands were similar The SPECT images utilized ROIs over striatum (which reflect DA transporters), midbrain (previously shown for {beta}-CIT to reflect primarily serotonin transporters), and the occipital lobe (a region of non-specific uptake). The time to peak specific striatal uptake (striatal minus occipital activity) was similar for IP-CIT and {beta}-CIT (377{plus_minus}60 and 410{plus_minus}60 min, respectively); whereas midbrain peak activity occurred at a significantly earlier time for IP-CIT (21{plus_minus}4 min) as compared to {beta}-CIT (60{plus_minus}17 min). At time of peak specific striatal activity, striatal to occipital ratios were 2.7+0.6 for IP-CIT and 7.6{plus_minus}0.7 for {beta}-CIT, and at time of peak midbrain activity, midbrain to occipital ratios were 1.1{plus_minus}0.1 for IP-CIT, and 1.7{plus_minus}0.2 for {beta}-CIT. At peak specific striatal time, normalized regional uptake values ({mu}Ci/cc per {mu}Ci injected dose per g body mass) for the striatum were 4.9{plus_minus}1.1 IP-CIT and 5.2{plus_minus}0.7 {beta}-CIT, whereas for the occipital lobe normalized regional uptake values were 1.9{plus_minus}0.4 IP-CIT and 0.7{plus_minus}0.2 for {beta}-CIT. Similar regional kinetics in the striatum were observed, as both ligands demonstrate comparable peak striatal uptake and time to peak.

  18. Evaluation of the redistribution in [sup 123]I-IMP SPECT in cerebrovascular disease

    Energy Technology Data Exchange (ETDEWEB)

    Matsumoto, Masahiro; Shibasaki, Takashi; Horikoshi, Satoru; Ohye, Chihiro; Suzuki, Hideki (Gunma Univ., Maebashi (Japan). School of Medicine)

    1994-06-01

    We evaluated the correlation between redistribution on IMP SPECT and the regional oxygen extraction fraction (rOEF) on PET. Ten lesions that had shown the redistribution phenomenon on IMP SPECT in five patients were selected. In four patient, IMP SPECT was performed within 2 months of the onset of cerebrovascular attack. In one patient, the examination was performed 3 years after the onset. The interval between IMP SPECT and PET was 45 days, except in one patient who was in the chronic phase. The ten lesions were classified into two groups according to rOEF values as follows: Group 1, rOEF[>=]50% and Group 2, rOEF<50%. In group 1, all lesions did not show low density on final X-ray CT. By contrast, in group 2, three lesions did not show low density on the X-ray CT and the other two lesions did. Redistribution rate (RD rate) was defined as follows, RD rate= [l brace](B-A) /B- (B'-A') /B'[r brace] / (B-A) /Bx100 (%) where, A, B, A' and B' are count rate in each of the regions of interest (ROIs). Region of interest (ROI) A was positioned in the lesion on the early image of SPECT that showed redistribution on the delayed image and ROI B was in the opposite side or occipital lobe (normal brain). On the delayed image, ROI A' and B' were positioned at the same place as A and B. ROIs on PET were positioned in the lesion corresponding to ROI A and B on SPECT. In group 1, all RD rates increased more than 70%. By contrast, in group 2, most RD rates were not more than 70%. In group 1, the A'/B' ratio increased more than A/B and was always greater than 90%. It is suggested that the presence of the redistribution phenomenon on IMP SPECT dose not always show the tissue viability. But quantification of the phenomenon by calculating A'/B' or RD rate is useful in evaluating tissue viability of the lesions and in predicting the final outcome of the area. (author).

  19. Density of striatal D2 receptors in untreated first-episode psychosis: an I123-IBZM SPECT study.

    Science.gov (United States)

    Corripio, Iluminada; Escartí, María J; Portella, Maria J; Pérez, Víctor; Grasa, Eva; Sauras, Rosa B; Alonso, Anna; Safont, Gemma; Camacho, M Valle; Dueñas, Rosa; Arranz, Belén; San, Luis; Catafau, Ana M; Carrió, Ignasi; Alvarez, Enric

    2011-12-01

    There is as yet no definite prognostic marker to determine whether a first-episode psychosis will become schizophrenia or not. The aim of the present study is to address whether the mechanism of sensitization of the subcortical dopaminergic pathway - yielding to an increase of the postsynaptic D2 receptors - may serve as a prognostic marker of clinical outcome in drug naïve patients with a first-episode psychosis, by means of a prospective and multicentric study with untreated first-episode psychosis patients (n=37). 123I-IBZM SPECT was performed at the time of the inclusion in the study, before antipsychotic medication was initiated. One year later, patients were assessed again so as to determine their diagnosis. There was a significant group effect at baseline in D2 Striatal/Frontal (S/F) ratios (F=10.2, p<0.001). Bonferroni posthoc comparisons attested significant differences between diagnosis (p=0.006), and between schizophrenia and control groups (p<0.001) but no differences between non-schizophrenia and control groups (p=0.9). The logistic regression model showed that D2R binding (p=0.02) and PAS (Premorbid Adjustment Scale) adulthood score (p=0.03) were predictive of the final diagnosis (schizophrenia/non-schizophrenia; Nagelkerke R(2)=0.59; X(2)=11.08, p=0.001). These findings replicate previous results on the usefulness of D2R binding as an objective prognostic parameter, together with the evaluation of premorbid adjustment, of the evolution of first-episode psychosis. In this regard, the results may provide a new view in the approach of early and personalized treatment in the debut of a psychosis. Copyright © 2011 Elsevier B.V. and ECNP. All rights reserved.

  20. Cerebral perfusion changes in traumatic diffuse brain injury. IMP SPECT studies

    Energy Technology Data Exchange (ETDEWEB)

    Ito, Hiroshi; Kawashima, Ryuta; Fukuda, Hiroshi [Tohoku Univ., Sendai (Japan). Inst. of Development, Aging and Cancer; Ishii, Kiyoshi; Onuma, Takehide

    1997-05-01

    Diffuse brain injury (DBI) is characterized by axonal degeneration and neuronal damage which cause diffuse brain atrophy. We have investigated the time course of abnormalities in cerebral perfusion distribution in cases of DBI by using Iodine-123-IMP SPECT, and the relationship to the appearance of diffuse brain atrophy. SPECT scans were performed on eight patients with diffuse brain injury due to closed cranial trauma in acute and chronic stages. All patients showed abnormalities in cerebral perfusion with decreases in perfusion, even in non-depicted regions on MRI, and the affected areas varied throughout the period of observation. Diffuse brain atrophy appeared in all patients. In some patients, diffuse brain atrophy was observed at or just after the time when the maximum number of lesions on SPECT were seen. The abnormalities in cerebral perfusion in cases of DBI might therefore be related to axonal degeneration and neuronal damage which causes diffuse brain atrophy. (author)

  1. Cerebral blood perfusion in febrile convulsions with sup 123 -IMP SPECT

    Energy Technology Data Exchange (ETDEWEB)

    Michihiro, Narumi; Ariizumi, Motomizu; Shiihara, Hiroaki; Suemitsu, Tomoko; Kurosawa, Yumiko; Egami, Yuriko (Dokkyo University Hospital, Saitama (Japan))

    1990-04-01

    Twenty-five pediatric patients with febrile convulsions underwent single photon emission computed tomography (SPECT) with N-isopropyl-p-(I-123) iodoamphetamine. Nineteen patients (76%) had a localized, decreased blood flow on early images, 12 of whom had it in the temporal region. According to seizure type, the abnormality was seen in 85% (17/20) for complex type, in contrast to 40% (2/5) for simple type. This finding has implications for pathological similarities between epilepsy and febrile convulsion. Delayed imaging performed on 12 patients showed redistribution in 5 of 9 patients having a decreased blood flow on early images. In the other 3 patients having normal findings on early images, however, different findings were seen on delayed images - normal, increased or decreased blood flow. There was no correlation between SPECT and encephalographic findings. A localized, decreased blood flow on SPECT seemed to reflect the risk for developing epilepsy. A follow-up care for preventing epilepsy is mandatory when there are abnormal findings on SPECT. (N.K.).

  2. [{sup 123}I]-IMP SPECT findings in spinocerebellar ataxia type 6

    Energy Technology Data Exchange (ETDEWEB)

    Ito, Yasuhiko; Nakabayashi, Haruo; Iguchi, Yasuyuki; Suzuki, Masahiko; Kobayashi, Masayuki [Jikei Univ., Chiba (Japan). Kashiwa Hospital; Nakajima, Takashi

    2000-01-01

    To study the dynamics of metabolic function in the cerebellar hemispheres, vermis and brain stem of patients with spinocerebellar ataxia type 6 (SCA6), we used single photon emission computed tomography (SPECT) with N-isopropyl-p-[{sup 123}I] iodoamphetamine (IMP) to measure regional cerebral blood flow (rCBF) in six Japanese patients with SCA6 and nine normal control subjects. All patients with SCA6 were found to have expanded CAG repeats (from 22 to 24 repeats). The SPECT data were also analyzed semiquantitatively. The rCBF in the cerebellar hemisphere, vermis and brain stem was not significantly lower in patients with SCA6 than in normal controls. However, the ratio of the cerebellar hemisphere to occipital lobe (C/O ratio) was significantly lower in patients. The ratio of the vermis and brain stem to occipital lobe (V/O, P/O ratio) were not significantly lower in patients. The C/O, V/O and P/O ratio were especially sensitive indexes for regional cerebral function in patients with SCA6. Results of this study suggest that the functional decrease in SCA6 may begin in the cerebellar hemispheres. IMP SPECT was useful for evaluating rCBF in patients with SCA6. (author)

  3. Study of sup 123 I-IMP SPECT on diabetic patients

    Energy Technology Data Exchange (ETDEWEB)

    Kinoshita, Ikuo; Takeo, Goh; Iwanaga, Keisuke; Uotani, Shigeo; Nakamura, Minoru; Sohda, Masanori; Ohe, Haruto; Toshimitsu, Takashi; Ohe, Nobuharu (Yahata City Hospital, Kitakyushu, Fukuoka (Japan))

    1991-07-01

    The involvement of peripheral nerves and nerve roots often leads to neurological manifestations which have frequently been described in association with diabetes mellitus. Whether there is any specific involvement of the central nervous system in this process has yet to be determined. Recently, many reports have suggested that significant neurophysiologic abnormalities in the central nervous system can sometimes be found in diabetic patients. In order to accurately examine the existence of central nervous system involvement in patients with diabetes mellitus, comparisons of {sup 123}I-IMP (IMP) washout rates were made between normal adults (n=19, average age 43.3 years) and diabetic patients (n=23, average age 43.3 years), and these results were graphically demonstrated by color images. Early images were obtained 30 minutes after intravenous injection, while delayed images were made 4 hours after injection. The IMP washout rate was obtained by subtracting the values of the delayed image with the early image. The standard deviation (SD) of the IMP washout rate for each patient was compared to the averaged SD obtained from healthy adults. After calculating the deviation from SD levels of healthy adults, we made an image of the patient's IMP washout rates. These images were divided into seven degrees (I, II: normal, III, IV: borderline, V{approx}VII: abnormal) and the ratio of each degree was expressed by a histogram in each cerebral hemisphere as the washout rate index. In 23 diabetic subjects, seven patients were found to be borderline while sixteen patients were abnormal. These impairments were not related either to the presence of diabetic triopathy or the duration of disease. By utilising such a noninvasive method, we were able to accurately evaluate the extent of diabetic central neuropathy. Therefore this subtraction method, which uses the specificity of IMP SPECT, is thus considered to be highly useful for broad clinical application. (author).

  4. sup 201 Tl SPECT for evaluation of brain tumors as compared with sup 123 I-IMP SPECT and sup 18 F-FDG PET

    Energy Technology Data Exchange (ETDEWEB)

    Oriuchi, Noboru (Gunma Univ., Maebashi (Japan). School of Medicine)

    1991-01-01

    Clinical usefulness of {sup 201}Tl SPECT for evaluation of brain tumors was studied in comparison with {sup 123}I-IMP SPECT and {sup 18}F-FDG PET. {sup 201}Tl SPECT and {sup 123}IMP SPECT were performed in 73 patients with brain neoplasm (group 1) and 15 patients with non-tumor cerebral diseases (group 2). Among them, 31 patients in group 1 and 5 patients in group 2 received {sup 18}F-FDG PET. SPECT was done with a ring type machine (HEADTOME SET011) and PET with a neuro PET (PCT H1). Forty-eight of 73 (65.8%) patients in group 1 showed increased accumulation of {sup 201}Tl in tumor site, whereas only 9 (12.3%) patients showed increased radioactivity of {sup 123}I-IMP in the lesion. Eighteen of 31 (58.1%) patients with neoplasm demonstrated increased regional cerebral metabolic rate of glucose (rCMRgl) in the lesion. Only two out of 15 (13.3%) patients with non-tumor lesion demonstrated increased accumulation of {sup 201}Tl. None of them showed accumulation of {sup 123}I-IMP or increased rCMRgl. Patients with malignant neoplasm demonstrated higher uptake of {sup 201}Tl in the lesion than those with benign neoplasm or non-tumor lesions. Post-therapeutic patients with glioblastoma or metastatic tumors showed lower {sup 201}Tl uptake than before therapy. Superimposed images of both {sup 201}Tl and {sup 123}I-IMP resemble rCMRgl functional images, suggesting increased {sup 201}Tl radioactivity in viable tumor tissue with decreased radioactivity in surrounding areas. {sup 201}Tl SPECT is useful for diagnosis of brain tumors and evaluation of effectiveness of therapy or evidence of residual tumor tissue. It may differentiate malignant tumors from benign tumors or non-tumor intracranial diseases including radiation injury and detect tumor recurrence earlier. (author).

  5. A simplified double-injection method to quantify cerebral blood flow and vascular reserve using iodine-123 IMP-SPECT

    Energy Technology Data Exchange (ETDEWEB)

    Toyoda, Hiroshi; Nishizawa, Sadahiko; Shiozaki, Toshiki; Ueno, Makoto; Konishi, Junji [Kyoto Univ. (Japan). Graduate School of Medicine

    2002-04-01

    We developed and evaluated a simplified double-injection method for iodine-123 N-isopropyl-p-iodoamphetamine (IMP) to quantify regional cerebral blood flow (rCBF) twice in a single SPECT session. The method enabled rapid calculations of rCBF with five 10-minute SPECT scans, a fixed distribution volume (V{sub d}), and one-point arterial blood sampling to calibrate a standard input function (SIF). Sixty neurological patients were examined to measure rCBF twice in a single session of IMP-SPECT. Patients underwent frequent arterial blood sampling with two injections of IMP and acetazolamide challenge. We generated the SIF and determined the optimal V{sub d} and calibration time (t{sub cal}) for the SIF in 30 patients. Validities of the fixed t{sub cal} and V{sub d} were assessed in the remaining 30 patients. Simulation studies were also performed to evaluate the error sensitivity of the method. The optimal t{sub cal} and V{sub d} were 34 min and 30 ml/ml, respectively. The method was robust in rCBF calculation with noisy SPECT data and yielded rCBF with negligible bias and acceptable errors compared with those obtained by the double-injection method previously reported. The method can be applied to measure rCBF twice in a single SPECT session more easily and less invasively. (author)

  6. Brain imaging with sup 123 I-IMP-SPECT in migraine between attacks

    Energy Technology Data Exchange (ETDEWEB)

    Schlake, H.P.; Boettger, I.G.G.; Grotemeyer, K.H.; Husstedt, I.W.

    1989-06-01

    {sup 123}I-IMP-SPECT brain imaging was performed in patients with classic migraine (n = 5) and migraine accompagnee (n = 18) during the headache-free interval. A regional reduction of tracer uptake into brain was observed in all patients with migraine accompagnee, while in patients with classic migraine only one case showed an area of decreased activity. The most marked alteration was found in a patient with persisting neurological symptoms (complicated migraine). In most cases the areas of decreased tracer uptake corresponded to headache localization as well as to topography of neurologic symptoms during migraine attacks. It may be concluded that migraine attacks occur in connection with exacerbations of preexisting changes of cerebral autoregulation due to endogenous or exogenous factors.

  7. Simultaneous Tc-99m/I-123 Dual Radionuclide Myocardial Perfusion/Innervation Imaging Using Siemens IQ-SPECT with SMARTZOOM Collimator

    OpenAIRE

    Du, Yong; Bhattacharya, Manojeet; Frey, Eric C.

    2014-01-01

    Simultaneous dual-radionuclide myocardial perfusion/innervation SPECT imaging can provide important information about mismatch between scar tissue and denervated regions. The Siemens IQ-SPECT system developed for cardiac imaging uses a multifocal SMARTZOOM collimator to achieve a four-fold sensitivity for the cardiac region compared to a typical parallel-hole low-energy high-resolution collimator but without the data truncation that can result with conventional converging-beam collimators. Th...

  8. Simultaneous Tc-99m/I-123 dual-radionuclide myocardial perfusion/innervation imaging using Siemens IQ-SPECT with SMARTZOOM collimator

    Science.gov (United States)

    Du, Yong; Bhattacharya, Manojeet; Frey, Eric C.

    2014-06-01

    Simultaneous dual-radionuclide myocardial perfusion/innervation SPECT imaging can provide important information about the mismatch between scar tissue and denervated regions. The Siemens IQ-SPECT system developed for cardiac imaging uses a multifocal SMARTZOOM collimator to achieve a four-fold sensitivity for the cardiac region, compared to a typical parallel-hole low-energy high-resolution collimator, but without the data truncation that can result with conventional converging-beam collimators. The increased sensitivity allows shorter image acquisition times or reduced patient dose, making IQ-SPECT ideal for simultaneous dual-radionuclide SPECT, where reduced administrated activity is desirable in order to reduce patient radiation exposure. However, crosstalk is a major factor affecting the image quality in dual-radionuclide imaging. In this work we developed a model-based method that can estimate and compensate for the crosstalk in IQ-SPECT data. The crosstalk model takes into account interactions in the object and collimator-detector system. Scatter in the object was modeled using the effective source scatter estimation technique (ESSE), previously developed to model scatter with parallel-hole collimators. The geometric collimator-detector response was analytically modeled in the IQ-SPECT projector. The estimated crosstalk was then compensated for in an iterative reconstruction process. The new method was validated with data from both Monte Carlo simulations and physical phantom experiments. The results showed that the estimated crosstalk was in good agreement with simulated and measured results. After model-based compensation the images from simultaneous dual-radionuclide acquisitions were similar in quality to those from single-radionuclide acquisitions that did not have crosstalk contamination. The proposed model-based method can be used to improve simultaneous dual-radionuclide images acquired using IQ-SPECT. This work also demonstrates that ESSE scatter

  9. Simultaneous Tc-99m/I-123 Dual Radionuclide Myocardial Perfusion/Innervation Imaging Using Siemens IQ-SPECT with SMARTZOOM Collimator

    Science.gov (United States)

    Du, Yong; Bhattacharya, Manojeet; Frey, Eric. C.

    2014-01-01

    Simultaneous dual-radionuclide myocardial perfusion/innervation SPECT imaging can provide important information about mismatch between scar tissue and denervated regions. The Siemens IQ-SPECT system developed for cardiac imaging uses a multifocal SMARTZOOM collimator to achieve a four-fold sensitivity for the cardiac region compared to a typical parallel-hole low-energy high-resolution collimator but without the data truncation that can result with conventional converging-beam collimators. The increased sensitivity allows shorter image acquisition times or reduced patient dose, making IQ-SPECT ideal for simultaneous dual-radionuclide SPECT, where reduced administrated activity is desirable in order to reduce patient radiation exposure. However, crosstalk is a major factor affecting the image quality in dual-radionuclide imaging. In this work we developed a model-based method that can estimate and compensate for the crosstalk in IQ-SPECT data. The crosstalk model takes into account interactions in the object and collimator-detector system. Scatter in the object was modeled using the effective source scatter estimation technique (ESSE), previously developed to model scatter with parallel-hole collimators. The geometric collimator detector response was analytically modeled in the IQ-SPECT projector. The estimated crosstalk was then compensated for in an iterative reconstruction process. The new method was validated with data from both Monte Carlo simulation and physical phantom experiments. The results showed that the estimated crosstalk was in good agreement with simulated and measured results. After model-based compensation the images from simultaneous dual-radionuclide acquisitions were similar in quality to those from single radionuclide acquisitions that did not have crosstalk contamination. The proposed model-based method can be used to improve simultaneous dual-radionuclide images acquired using IQ-SPECT. This work also demonstrates that ESSE scatter modeling

  10. Comparison of [{sup 18}F]FDG-PET and L-3[{sup 123}I]-iodo-{alpha}-methyl tyrosine (I-123 IMT)-SPECT in primary lung cancer

    Energy Technology Data Exchange (ETDEWEB)

    Willkomm, P.; Bangard, M.; Guhlke, S.; Sartor, J.; Bender, H.; Gallkowski, U.; Reichmann, K.; Biersack, H.J. [Bonn Univ. (Germany)

    2002-11-01

    The aim of this study was to evaluate L-3[{sup 123}I]-iodo-{alpha}-methyl tyrosine (IMT)-SPECT and FDG-PET in pulmonary lesions suspected to be lung cancer. Whole body PET (measured transmission corrected emission scans) was performed 45 minutes after intravenous (i.v.) injection of 222-370 MBq (6-10 mCi) {sup 18}F-FDG on a Siemens PET scanner (ECAT EXACT 47) including 5-6 bed positions. {sup 123}I-IMT-SPECT (chest) was performed after injection of 370 MBq (10 mCi) with a dual head camera (Picker Prism 2000) and commercially available reconstruction algorithms. Ten patients (6 male and 4 female) with suspected lung cancer were investigated. The results were compared to histological findings after surgery or bronchoscopic biopsies and CT. {sup 123}I-IMT-SPECT and FDG-PET were able to detect all 9 cases of lung cancer (1-8 cm in diameter). One case was true negative. Both imaging methods were true positive with respect to mediastinal lymph node metastases in one patient. The tumor/background ratio was higher with PET (8.20 vs. 2.84). Despite the limited number of patients it may be concluded that IMT-SPECT as well as FDG-PET are suited to correctly diagnose lung cancer. Nevertheless, FDG-PET, if available, seems to be better suited because of the higher tumor/background ratio and better resolution. (author)

  11. Early differential diagnosis between Alzheimer's disease and dementia with Lewy bodies: Comparison between (18)F-FDG PET and (123)I-IMP SPECT.

    Science.gov (United States)

    Chiba, Yuhei; Iseki, Eizo; Fujishiro, Hiroshige; Ota, Kazumi; Kasanuki, Koji; Suzuki, Masaru; Hirayasu, Yoshio; Arai, Heii; Sato, Kiyoshi

    2016-03-30

    Both (18)F-fluorodeoxyglucose (FDG) positron emission tomography (PET) and (123)I-iodoamphetamine (IMP) single-photon emission computed tomography (SPECT) have been used for the differential diagnosis of Alzheimer's disease (AD) and dementia with Lewy bodies (DLB). Less information is available, however, regarding the differential diagnosis of mild cognitive impairment (MCI) due to AD and MCI due to DLB. We examined nine AD patients (AD group), nine DLB patients (DLB group), eight MCI due to AD patients (MCI-AD group), and nine MCI due to DLB patients (MCI-DLB group) with FDG PET and IMP SPECT using a well-characterized normal database and a stereotactic extraction estimation method. In the AD and DLB groups, receiver operating characteristic (ROC) analysis in the occipital regions showed significant accuracy of both FDG PET and IMP SPECT for the differential diagnosis. In the MCI-AD and MCI-DLB groups, ROC analysis showed significant accuracy of only FDG PET for the differential diagnosis. Both FDG PET and IMP SPECT would be useful for the differential diagnosis between AD and DLB. For the differential diagnosis of MCI-AD versus MCI-DLB, FDG PET would be more useful than IMP SPECT.

  12. SPECT with N-isopropyl-p iodoamphetamine in occlusive cerebrovascular diseases.

    Science.gov (United States)

    Higa, T; Tanaka, T; Ikekubo, K; Komatsu, T; Torizuka, K

    1986-12-01

    The role of SPECT imaging with N-isopropyl-p iodoamphetamine (I-123 IMP) in the detection of angiographically documented occlusive cerebrovascular diseases was evaluated in 24 patients, and the results of regional cerebral blood flow (rCBF) were compared with x-ray CT. Twelve patients had internal carotid occlusion, ten had intracranial occlusion beyond the circle of Willis, one had common carotid occlusion, and one had basilar artery occlusion. SPECT images were obtained with a gamma camera, which was rotated 360 degrees around the patient's head 30 minutes after an intravenous injection of 3 mCi of I-123 IMP. CT images in the transverse plane were obtained, and the regions of reduced attenuation were identified for comparison of topographic extension of the lesion with the regions of decreased rCBF seen on SPECT. In six cases, the lesions seen on the SPECT images were distinctly more extensive than those seen on CT. In the remaining 18 cases, the extent of the lesion was identical on both CT and SPECT images. Radiochemical and radionuclide impurities, the distance of the detector from the head, and the nature of the collimator affected the SPECT results. I-123 IMP SPECT imaging complements CT findings in detecting the ischemic zones beyond the regions identified on CT images, and may have a major rule in the management of patients with occlusive cerebrovascular diseases.

  13. Normal CBF values by the ARG method using IMP SPECT. Comparison with a conventional microsphere model method

    Energy Technology Data Exchange (ETDEWEB)

    Ito, Hiroshi; Koyama, Masamichi; Kawashima, Ryuta; Ono, Shuichi; Fukuda, Hiroshi [Tohoku Univ., Sendai (Japan). Inst. of Development, Aging and Cancer; Ishii, Kiyoshi; Kinoshita, Toshifumi

    1996-02-01

    N-isopropyl-p[{sup 123}I]iodoamphetamine (IMP) has been used as a flow tracer for SPECT, and measurement of cerebral blood flow (CBF) using IMP has been performed by conventional microsphere model method (MS method). Recently, the ARG method for measuring CBF by using IMP with one SPECT scan and one point blood sampling has been developed. This method was based on two-compartment model. In the present study, normal CBF values were measured in 10 healthy male subjects (mean age{+-}S.D.: 29.8{+-}6.01, age range: 23-41) by ARG and MS methods. The mean CBF values ({+-}S.D.) for ARG method in which V{sub d} value was assumed to be 50 ml/ml were 41.7{+-}9.4, 31.1{+-}5.0, 40.7{+-}9.7, 41.5{+-}10.0, 38.2{+-}9.2, 39.0{+-}9.4, 41.9{+-}10.6, 38.7{+-}8.0 and 30.0{+-}7.7 ml/100 ml/min in the cerebellum, pons, thalamus, basal ganglia, frontal, temporal, parietal, occipital lobe cortex and centrum semiovale, respectively. The mean CBF values for the MS method were 46.8{+-}8.4, 37.5{+-}5.6, 45.8{+-}8.6, 46.5{+-}8.9, 43.7{+-}8.3, 44.4{+-}8.7, 46.8{+-}9.3, 44.3{+-}7.3 and 36.3{+-}8.1 ml/100 ml/min, respectively. The mean CBF values in the cerebral cortex region for ARG method were lower than those previously reported by PET. This would be caused by low first-pass extraction fraction of IMP compared with oxygen-15 labeled water. The mean CBF values for the MS method were higher than those for ARG method against previous studies. As reasons for this, errors in estimation of SPECT brain counts at 8 min in the MS method were considered. (author).

  14. Reproducibility of regional cerebral blood flow measurement at rest with autoradiography method using {sup 123}I-IMP and SPECT

    Energy Technology Data Exchange (ETDEWEB)

    Saito, Kyoko; Murata, Hajime; Maruno, Hirotaka; Mori, Kazuaki; Tsuchida, Shoichi [Toranomon Hospital, Tokyo (Japan)

    1997-05-01

    Reproducibility of regional cerebral blood flow (rCBF) values obtained by the autoradiography (ARG) method with one SPECT scan and one point arterial blood sampling at 10 min after {sup 123}I-N-isopropyl-p-iodoamphetamine (IMP) infusion was evaluated. The variation in the cross calibration factor between SPECT counts and well-type scintillation counts remained 2.2% for 13 months. The error in rCBF by sampling time difference was less than 3% when sampling time was within 10 min{+-}30 sec after infusion. There was a favorable correlation between the rCBF values in two separate resting scans measured by the ARG method in 10 patients (r=0.907). And the mean ratio of rCBF values by the second study to those by the first study in the cerebellum and cerebral lobes in 10 patients was 0.989{+-}0.056 (mean{+-}SD). There was no significant difference in rCBF values between two separate studies. Reproducibility of rCBF with the ARG method was proved to be good. The ARG method was thought to be useful for the assessment of treatment effects and clinical courses. (author)

  15. Analysis of cognitive function and regional cerebral blood flow in Parkinson's disease by {sup 123}I-IMP SPECT

    Energy Technology Data Exchange (ETDEWEB)

    Kato, Takayuki; Morohoshi, Toshio; Hasegawa, Yukihiro; Fukui, Toshiya; Kawamura, Mitsuru; Sugita, Koujiro [Showa Univ., Tokyo (Japan). School of Medicine

    2000-06-01

    Several studies have found that the uptake of a radioisotope is reduced in the frontal or parietooccipital lobe on SPECT scans of patients with Parkinson's disease accompanied by cognitive impairment. The present study investigated the relationship between the results of SPECT imaging and those of cognitive function tests in patients with Parkinson's disease accompanied by cognitive impairment. Thirty patients with Parkinson's disease served as subjects, including 9 men and 21 women with an average of 66.1 years. Patients underwent {sup 123}I-IMP SPECT and various cognitive function tests (Japanese alphabet pick-up test, Stroop test, Hasegawa's dementia screening scale, Raven color matrix, and course cube-combination test). Next, three neurologists with no knowledge of the patient's clinical symptoms were asked to analyze the reduced uptake on SPECT scans as follows: frontal lobe, parietooccipital lobe, frontal and parietooccipital lobes, diffused reduction, and no reduced uptake. The results of SPECT imaging and each cognitive function test were compared. Reduced uptake in the frontal and parietooccipital lobes and diffused reduction correlated with decreases in cognitive function. Cognitive impairment is not notable when cortical blood flow is partially reduced, but is notable when cortical blood flow is widely reduced. (author)

  16. [Complex partial status epilepticus with recurrent episodes of complex visual hallucinations: study by using 123I-IMP-SPECT, brain MRI and EEG].

    Science.gov (United States)

    Sakai, Toshiyuki; Kondo, Masahide; Tomimoto, Hidekazu

    2015-01-01

    We report a 72-year-old woman with complex partial status epilepticus who showed recurrent episodes of complex visual hallucinations (CVH). Brain diffusion-weighted magnetic resonance images revealed gyriform cortical hyperintensity in the right parietal, occipital and temporal lobes, and brain magnetic resonance angiograhy revealed a hyperintensity in the right dilated middle cerebral artery during ictal period. Ictal N-isopropyl-p-(iodine-123)-iodoamphetamine single photon emission computed tomography (123I-IMP-SPECT) with three-dimensional stereotactic surface projection (3D-SSP) 14 days after the onset of the first CVH revealed hyperperfusion in the right latero-inferior occipito-temporal region with relation to motion. CVH spontaneously subsided 17 days after the onset of the first CVH. CVH recurred one year after the first CVH. Ictal 123I-IMP-SPECT with 3D-SSP revealed marked hyperperfusion in the right lateral parietal region probably with relation to face and figure hallucinations. Ictal scalp EEGs revealed rhythmic polyspikes at 12 Hz with high amplitude (100-200 μV) in bilateral posterior occipital and temporal region with the right side dominance for 20 seconds and more in several occasions. Interictal 123I-IMP-SPECT with 3D-SSP 28 days after recurrence of CVH revealed marked hypoperfusion in the right lateral parietal region, and recovery of hypoperfusion in the right latero-inferior occipito-temporal region. These findings suggest that ictal CVH might be induced by the spread of epileptic discharges from the right parieto-occipito-temporal region with the old brain contusion (epileptogenic region) to the right latero-inferior occipito-temporal region and the right lateral parietal region (symptomatogenic regions).

  17. A comparative study of simple methods to quantify cerebral blood flow with acetazolamide challenge by using iodine-123-IMP SPECT with one-point arterial sampling

    Energy Technology Data Exchange (ETDEWEB)

    Ohkubo, Masaki [Niigata Univ. (Japan). School of Health Sciences; Odano, Ikuo

    2000-04-01

    The aim of this study was to compare the accuracy of simplified methods for quantifying rCBF with acetazolamide challenge by using {sup 123}I-N-isopropyl-p-iodoamphetamine (IMP) and SPECT with one-point arterial sampling. After acetazolamide administration we quantified rCBF in 12 subjects by the following three methods: (a) the modified microsphere method, (b) the IMP-autoradiographic (ARG) method based on a two-compartment one-parameter model, and (c) the simplified method based on a two-compartment two-parameter model (functional IMP method). The accuracy of these methods was validated by comparing rCBF values with those obtained by the standard method: the super-early microsphere method with continuous withdrawal of arterial blood. On analyzing rCBF in each flow range (0-0.25, 0.25-0.5, 0.5-0.75 and more than 0.75 ml/g/min), rCBF values obtained by both methods (a) and (c) showed significant correlations (p<0.01) with those obtained by the standard method in every range, but rCBF values obtained by method (b) did not significantly correlated in the high flow range (0.5-0.75 and more than 0.75 ml/g/min). Method (c) was found to be the most accurate, even though it needs two serial SPECT scans. When requiring one SPECT scan, method (a) was considered to be superior to method (b) because of its accuracy, especially in high flow regions loaded with acetazolamide. (author)

  18. A comparative study of the quality of SPECT images obtained by {sup 123}I-IMP, {sup 99m}Tc-HMPAO and {sup 99m}Tc-ECD

    Energy Technology Data Exchange (ETDEWEB)

    Odano, Ikuo; Takahashi, Makoto; Noguchi, Eikichi; Ohtaki, Hiro; Kasahara, Toshifumi; Shibaki, Mitsurou; Hatano, Masayoshi [Niigata Univ. (Japan). School of Medicine; Ohkubo, Masaki

    1997-03-01

    The purpose of this study was to comparatively evaluate the quality of SPECT images for the mapping of rCBF using three tracers, {sup 123}I-IMP, {sup 99m}Tc-HMPAO and {sup 99m}Tc-ECD. We performed three SPECT studies on seven patients with various cerebral diseases under the same conditions. An effect of Lassen`s correction on SPECT images obtained by HMPAO was also evaluated. The same irregular regions of interest were placed on the four transaxial SPECT images. To quantitatively evaluate the pattern of tracer uptake and image contrast, the uptake ratio, regional count/mean count of the cerebrum, and its coefficient variations (CV) were defined, respectively. The order of the value of CV was HMPAO with correction>IMP>ECD>HMPAO without correction. HMPAO with correction showed the best image contrast, but HMPAO without correction was the worst. Uptake ratios of ECD and HMPAO with correction were decreased in the brain stem and thalamus in comparison with those of IMP. Both uptake ratios of ECD and HMPAO without correction were increased in the occipital cortex. IMP provides high quality SPECT images. Images obtained by HMPAO should be modified by Lassen`s correction to increase image contrast. ECD or HMPAO should not be used to evaluate patients with spinocerebellar degeneration. (author)

  19. Parieto-occipital hypoaccumulation of {sup 123}I-IMP in the brain SPECT associated with maternal inheritance of diabetes mellitus

    Energy Technology Data Exchange (ETDEWEB)

    Suzuki, Yoshihiko; Atsumi, Yoshihito; Hosokawa, Kazuhiro; Shimada, Akira; Asahina, Takayuki; Matsuoka, Kempei [Saiseikai Central Hospital, Tokyo (Japan); Hata, Takashi; Taniyama, Matsuo

    1997-07-01

    To determine the latent effect of diabetes inheritance on central nervous system, thirty diabetic patients were examined (14 male, 16 female). Seventeen patients had a mother with diabetes, and the other thirteen had non-diabetic mothers. They were previously determined to not have the 3243 mitochondrial tRNA mutation in peripheral leukocytes. Patients were tested for parieto-occipital hypoaccumulation of {sup 123}I-IMP of brain SPECT, a characteristic neurofinding of mitochondrial diabetes mellitus due to the 3243 tRNA mutation. Seven (41.2%) out of 17 subjects with material inheritance had the parieto-occipital abnormality, whereas one (7.7%) out of 13 subjects with non-maternal inheritance had the abnormality. Seventeen (94.4%) out of 18 patients diabetes due to mitochondrial tRNA mutation at position 3243 showed the abnormality. Our results suggest that the material inheritance of diabetes is associated with the hypoaccumulation of {sup 123}I-IMP of brain SPECT. We speculate that, because the patients with maternal inheritance might have subclinical mitochondrial dysfunction due to unknown mitochondrial DNA abnormalities, the mitochondrial DNA abnormality might cause their subclinical brain damage in the parieto-occipital area. (author)

  20. Cerebral hemodynamic difference between early- and late-onset Alzheimer's disease by circumferential profile analysis with [sup 123]I-IMP brain SPECT

    Energy Technology Data Exchange (ETDEWEB)

    Arai, Hisayuki; Hanyu, Haruo; Abe, Shinei; Asano, Tetsuichi; Takasaki, Masaru; Suzuki, Takanari; Abe, Kimihiko (Tokyo Medical Coll. (Japan)); Katsunuma, Hideyo

    1992-10-01

    We conducted investigation to determine whether early- and late-onset Alzheimer's diseases differ pathophysiologically. Five patients with the early-onset (65 years and under) of the disease and 11 with the late-onset (65 years and over) of the disease were studied by single photon emission CT (SPECT) with N-isopropyl-p-[[sup 123]I]iodoamphetamine (IMP). Circumferential profile analysis (CPA) was performed to examine differences in the predominant hypoperfusion in the temporoparietal lobe, which is considered to be functionally damaged the most in Alzheimer's disease. The Xm values, calculated from gradients between the motorsensory or occipital cortices and temporoparietal cortex in the circumferential profile curve, were compared in both groups. The Xm values for patients with early- and late-onset Alzheimer's disease were 6.81[+-]2.10 (counts/degree) and 3.28[+-]1.58, respectively, the difference being significant. Our results suggest that functional abnormalities in the temporoparietal area severer in early- than late-onset Alzheimer's disease and that the application of CPA to IMP SPECT is useful to elucidate the pathophysiological difference between each of the disease. (author).

  1. Single photon emission computed tomography (SPECT) findings using N-isopropyl-p-[[sup 123]I]iodoamphetamine ([sup 123]I-IMP) in schizophrenia and atypical psychosis

    Energy Technology Data Exchange (ETDEWEB)

    Suga, Hidemichi (Aichi Medical Univ., Nagakute (Japan))

    1993-05-01

    Sixteen schizophrenic patients, 16 atypical psychosis patients, and 16 healthy volunteers were subjected to single photon emission computed tomography (SPECT) of the brain using N-isopropyl-p-[[sup 123]I]iodoamphetamine ([sup 123]I-IMP). The basal ganglia region was in particular examined not only in transverse sections, but in coronal sections. Schizophrenics showed significantly decreased uptake rates in the bilateral frontal regions and increased uptakes in the bilateral basal ganglia. On the other hand, atypical psychotics had a reduced uptake rate only in the right thalamic region, compared to the controls. The increased uptake rates in the basal ganglia were associated with auditory hallucination, but gender difference, duration of illness and dose of neuroleptics had no influence on these SPECT findings. The results suggest that schizophrenics might have some lesions in the frontal area of the brain, whereas atypical psychotics might have no lesion in the frontal region but dysfunction in the right thalamic region. Subsequently, using only SPECT findings, all the cases were divided by cluster analysis into 4 groups and a residue group. Schizophrenics distributed mainly in the 2 groups that have lesion in the frontal regions. Atypical psychotics distributed principally in the other 2 groups that have alterations in the bilateral thalamic region. The present study suggests that schizophrenia and atypical psychosis might have different etiologies. (author).

  2. Gyral high density on CT scan after head injury; [sup 123]I-IMP SPECT and MRI findings in three children

    Energy Technology Data Exchange (ETDEWEB)

    Abe, Takumi; Matsumoto, Kiyoshi (Showa Univ., Tokyo (Japan). School of Medicine); Sakamoto, Tetsuya; Aruga, Tohru

    1994-02-01

    The authors treated three children who had 'gyral high density' on plain CT scans after head injury with acute subdural hematoma. [sup 123]I-IMP SPECT (IMP) and MRI in the chronic stage were performed. All were males, about one year of age, with acute subdural hematoma. CT scan 48 hours after injury showed diffuse low density in the ipsilateral parenchyma with minimum midline shift, and IMP showed decreased activity in the same area. Plain CT scan 1 to 3 weeks after injury showed remarkably high density along the gyri in part of the same area. This area was markedly enhanced on CT with contrast medium and showed decreased blood flow on IMP. This high density area disappeared within 2 months after injury and the area concerned showed brain atrophy. In the chronic stage (after 6 months), only the high density area along the gyri seen in the CT scan showed MRI evidence of ishemia, but there was no definite evidence of hemorrhage. All three children had hemiplegia at the time of discharge. The gyral high density suggests ischemic brain, but the pathophysiological process might be different from that of so-called hemorrhagic infarction. Presumably, it is due to incomplete autoregulation, the incomplete blood-brain barrier and the sensitivity to stimulation of cerebral blood vessels in the brains of infants. The intensity and range of the gyral high density are considered to be important in estimating the future amount of atrophic change in the insulted brain and the resulting sequelae. (author).

  3. Assessment of cerebral hemodynamics before and after revascularization in patients with occlusive cerebrovascular disease by means of quantitative IMP-SPECT with double-injection protocol

    Energy Technology Data Exchange (ETDEWEB)

    Ueno, Makoto; Nishizawa, Sadahiko; Toyoda, Hiroshi; Shimono, Taro; Miyamoto, Susumu; Hashimoto, Nobuo; Konishi, Junji [Kyoto Univ. (Japan). Graduate School of Medicine

    2001-06-01

    The purpose of this study was to validate a double-injection (DI) method with N-isopropyl-[{sup 123}I]p-iodoamphetamine (IMP) to measure regional cerebral blood flow (rCBF) twice in a single session of dynamic SPECT and to elucidate a possible role of this method to identify patients with occlusive disease of major cerebral arteries, who might benefit from cerebral revascularization procedures (CR). Fourteen patients with occlusion or severe stenosis of the internal carotid or middle cerebral artery were studied before and after CR to assess hemodynamic changes after revascularization treatment. We quantitatively measured rCBF before and after acetazolamide (ACZ) challenge along with cerebrovascular reserve capacity (CVR) with two injections of IMP in a single session of dynamic SPECT scans (DI method). The reliability and reproducibility of the DI method were validated by means of a simulation study and in eight patients who were examined without ACZ challenge to measure baseline rCBF twice. The analysis of simulated noisy data with realistic noise levels showed that the errors of the estimates for the first and the second rCBF and for the increase in rCBF were 2.6%, 8.1% and 10.4%, respectively. In the 8 patients examined by the DI method to measure baseline rCBF twice, the mean and the SD of percentage differences between the two consecutive measurements in rCBF were -1.3% and 5.5%, respectively. Eight out of 14 patients with occlusive disease had at least one region with a CVR less than 10%. They showed a significant increase in resting rCBF after CR, not only in the ipsilateral hemisphere (from 26.1{+-}6.4 to 33.4{+-}4.7) but also in the contralateral one (from 28.3{+-}7.0 to 34.7{+-}4.7) with a recovery of the ipsilateral CVR from 9.3{+-}17.2 to 41.2{+-}20.1%. The remaining six patients with good-moderate CVR did not show an increase in rCBF after CR (from 28.0{+-}2.7 to 28.3{+-}3.4). The three of them with a moderate CVR (10-25%) before CR showed

  4. Dual myocardial single photon emission computed tomography (SPECT) using thallium-201 and I-123-{beta}-methyl-i-pentadecanoic acid in patients with Duchenne's progressive muscular dystrophy

    Energy Technology Data Exchange (ETDEWEB)

    Shimoyama, Katsuya [Kyorin Univ., Mitaka, Tokyo (Japan). School of Medicine

    1999-10-01

    Dual single photon emission computed tomography (SPECT) was performed in 31 patients with Duchenne's progressive muscular dystrophy (DMD) using {sup 123}I-{beta}-methyl pentadecanoic acid (BMIPP) for myocardial fatty acid metabolism and {sup 201}thallium (Tl)-chloride for myocardial perfusion. The left ventricle was divided into 9 segments, and accumulation of the radiotracers was assessed visually for each segment to calculate defect score for each tracer. There was some degree of decrease in myocardial accumulation of both tracers in all DMD patients. Reduced accumulation was most common at the apex (BMIPP: 67%, Tl: 63%), followed by the posterior wall, lateral wall, and anterior wall. On the other hand, reduced accumulation was less common at the septum. BMIPP showed a higher accumulation than Tl in all segments but the septum. When BMIPP defect score was larger than Tl defect score, BMIPP defect score tended to increase during 4 years follow-up (p<0.042). However, when Tl defect score was larger than BMIPP defect score, an increase in Tl defect score was slight. A significant negative correlation was found between the sum of the BMIPP and Tl defect scores and the left ventricular ejection fraction (LVEF) (r=0.66, p<0.0001). According to the histo-pathological study of two autopsied hearts, severe myocardial fibrosis was seen in segments with fixed perfusion defect. In addition, the mismatched segments of BMIPP defect score > Tl defect score revealed a slight fibrosis or normal myocardium. It can be concluded that the dual SPECT myocardial scintigraphy using BMIPP and Tl provides accurate information about disease progression of the heart in patients with DMD by detecting abnormalities of the myocardial metabolism of each substance, thereby enabling the assessment of left ventricular function. (author)

  5. Effect of cerebral blood flow on consciousness and outcome after head injury. Assessment by jugular bulb venous metabolism and IMP-SPECT

    Energy Technology Data Exchange (ETDEWEB)

    Imaizumi, Shigeki; Onuma, Takehide; Motohashi, Osamu; Kameyama, Motonobu; Ishii, Kiyoshi [Sendai City Hospital (Japan)

    2002-10-01

    This study was performed to elucidate the therapeutical value of arteriojugularvenous oxygen difference (AVDO{sub 2}) in the ultra-emergent period after head injury. Rational therapeutic strategy after severe head injury needs information concerning the dynamical change of cerebral blood flow (CBF) and metabolism. We monitored the cerebral venous metabolism within 6 hours after head injury until the day IMP-SPECT was performed. Whole brain cerebral blood flow detected by IMP-SPECT and AVDO{sub 2} at the same day was compared, which restored to the period within 6 hours after head injury. From this procedure, we could outline cerebral blood flow conditions by only AVDO{sub 2} without IMP-SPECT in the ultra-emergent period. Eighty-six patients with head injury who were carried to our emergency center in the period of recent 2 years aged ranging from 15 to 94 years were the subjects. They all performed jugular bulb cannulation within 6 hours after the accident (Martin's phase I: day 0) to know saturation of jugular vein (SjO{sub 2}), AVDO{sub 2} and AVL. They were monitored until the day IMP-SPECT was performed (Martin's phase II; day 1-3 or phase III; day 4-15). The correlation between CBF and AVDO{sub 2}. The effect of CBF and cerebral venous metabolism on consciousness and outcome was also analyzed. CBF and AVDO{sub 2} in phase II and III were reversely correlated (p<0.0001). Normal CBF corresponded with 5.0 vol% in AVDO{sub 2}. AVDO{sub 2} in all cases changed 6.2 vol% at phase I, 4.5 vol% at phase II and 5.1 vol% at phase III. Glasgow comascale (GCS) on admission under 8 (n=47) and over 9 (n=39) significantly differed in AVDO{sub 2} and CBF in the period of II and III. The patients with favorable consciousness showed low AVDO{sub 2} and hyperemia afterwards. Dead cases in phase I (n=19) showed high AVDO{sub 2} and low SjO{sub 2}. The patients with severe disability (SD) (n=13) showed high AVDO{sub 2} and low CBF and the patients with good recovery (GR

  6. Comparison of IMP-SPECT findings to subtest scores of Wechsler intelligence adult Scale-Revised in temporal lobe epilepsy patients

    Energy Technology Data Exchange (ETDEWEB)

    Kan, Rumiko; Uejima, Masahiko; Kaneko, Yuko; Miyamoto, Yuriko; Watabe, Manabu; Takahashi, Ruriko; Niwa, Shin-ichi; Shishido, Fumio [Fukushima Medical Coll. (Japan)

    1998-02-01

    In this study, 40 temporal lobe epilepsy patients were assessed, using the Laterality Index (LI) of ROI values in IMP-SPECT findings, Wechsler adult intelligence Scale-Revised (WAIS-R) and subtest scores. LIs of the frontal, temporal and occipital lobes were calculated as follows: the ROI values on the right side were subtracted from those on the left, and the results was divided by the sum of the ROI values on the right and left sides. The individual subtest scores on WAIS-R were standardized by all evaluation scores in order to exclude the influence of differences in intelligence level as much as possible. The results were as follows: there was a positive correlation (r=0.74, p<0.001) between LI values and the performance in Arithmetic in the left temporal lobe hypoperfusion group. And there was a positive correlation (r=0.50, p<0.02) between LI values and the performance in Vocabulary in the left temporal lobe hypoperfusion group. In the right occipital lobe hypoperfusion group, there was a negative correlation (r=-O.44, pSPECT might influence brain function. (author)

  7. Distribution of N-isopropyl-p-(I-123)iodoamphetamine among the peripheral blood components; An in vitro study

    Energy Technology Data Exchange (ETDEWEB)

    Kumazaki, Satoshi (Kanto Teishin Hospital, Tokyo (Japan)); Oriuchi, Noboru; Tomiyoshi, Katsumi; Inoue, Tomio; Sasaki, Yasuhito

    1990-03-01

    With the purpose to clarify dynamics of N-isopropyl-p-(I-123)iodoamphetamine (I-123 IMP) in the blood stream its binding to the peripheral blood components was determined by in vitro experiment. I-123 IMP was added to the peripheral venous blood obtained from healthy volunteers to be incubated for different length of time (0-30 min) at 37deg C. The blood was then separated into blood cells and plasma. From the latter platelet rich plasma were separated. Radioactivity in each blood component was counted in a well type scintillation counter respectively. To evaluate the affinity of I-123 IMP to red blood cell the component containing blood cells were washed repeatedly with salines. It was found that the fraction of radioactivity in the blood cell component was 68.0{plus minus}6.3% (m{plus minus}1 S.D.), which was higher than that in the plasma (32.0%{plus minus}6.3%). The radioactivity in the platelet-rich plasma was only 1.7{plus minus}1.1% of the total I-123 IMP activity. This percentage did not change by the incubation time. When Tc-99m DTPA was incubated with blood, radioactivity in the blood cell component was only 22.5%, which is further lowered by 32{plus minus}2.1% after each washing to reach 6.8% after three times washing. In contrast the radioactivity of I-123 IMP in blood cell component remained as high as 31.1% after eight times washing. Almost constant fraction (8.20{plus minus}0.57%) of radioactivity was freed into supernate by each washing. These findings suggest that a certain specific binding mechanism is involved in the binding of I-123 IMP to red blood cells. (author).

  8. An automated synthesis module for preparation of L-3-[I-123]iodo-alpha-methyl tyrosine

    NARCIS (Netherlands)

    Luurtsema, G; Jager, PL; Piers, A; de Hooge, MN

    2001-01-01

    L-3-[I-123]iodo-alpha-methyl tyrosine (IMT) is an artificial amino acid suitable for SPECT imaging of various tumours. Manual synthesis of this radiopharmaceutical is reliable, but time-consuming and may require handling of large quantities of radioactivity. We developed an automated IMT synthesis

  9. A rapid and simple automation of [I-123]mIBG for clinic application

    Energy Technology Data Exchange (ETDEWEB)

    Yi, Chi Hoon; Han, Sang Jin; Park, Hyun; Kim, Byung Soo; Cheon, Gi Jeong; Kim, Jung Young [Korea Institue of Radiological and Medical Sciences, Seoul (Korea, Republic of)

    2010-05-15

    Radioiodinated meta-iodobenzylquanidine (mIBG) as a functional analog of norepinephrine has been used in the clinical and diagnostic management of neroendocrine tumors like pheochromocytoma and neuroblastomas. In particular, [I-123]mIBG has diagnosed successfully as SPECT (Single Photon Emission Computed Tomography) imaging agent in nuclear medicine. The imaging ability of [I-123]mIBG is due to two effects of biological activity related to tumor and good physical terms (a half-life of 12.3 hours and a gamma energy of 140KeV ). Therefore, we can easily expect that a use of [I-123]mIBG will expand in Korean Hospitals. At that point, we are interested in an automatic and rapid preparation of [I-123]mIBG with good quality for clinic application

  10. Assessment of brain cell function using {sup 123}I-IMP SPECT of cerebral blood flow and visual evoked potential by photic stimulation in normal and demented elderly subjects

    Energy Technology Data Exchange (ETDEWEB)

    Ogawa, Kimikazu; Takasaki, Masaru [Tokyo Medical Coll. (Japan); Katsunuma, Hideyo; Ishida, Hitoshi

    1999-09-01

    Cerebral blood flow was quantitatively measured using {sup 123}I-IMP SPECT by photic stimulation and visual evoked potential (VEPs) in normal and dementia subjects: 8 with Alzheimer-type dementia, 9 with cerebrovascular dementia and 7 normal elderly subjects were divided into the three groups based on the Clinical Dementia Rating (CDR) grade: Group I (CDR 0), Group II (CDR 0.5-1), Group III (CDR 2-3). The {sup 123}I-IMP SPECT measurement was conducted at rest with the eyes closed and also during photic stimulation. VEPs were measured simultaneously. The results reveal prolongation of the P2 latency of the VEPs prolonged in accordance with the increasing severity of the dementia, and quantitative cerebral blood flow was lower in Group II and Group III than in Group I at rest, while during photic stimulation it significantly increased in Group I and II, but showed no change in Group III. The results suggest that quantitative measurement of cerebral blood flow using {sup 123}I-IMP SPECT by photic stimulation may enable more detailed assessment of brain cell function. (author)

  11. I-123/125-labelled 2-iodo-L-phenylalanine and 2-iodo-D-phenylalanine : comparative uptake in various tumour types and biodistribution in mice

    NARCIS (Netherlands)

    Kersemans, Veerle; Cornelissen, Bart; Kersemans, Ken; Bauwens, Matthias; Dierckx, Rudi A.; De Spiegeleer, Bart; Mertens, John; Slegers, Guido

    2006-01-01

    Purpose: In vitro in the R1M cell model and in vivo in the R1M tumour-bearing athymic model, both [I-123]- 2- iodo-L-phenylalanine and [I-123]-2-iodo-D-phenylalanine have shown promising results as tumour diagnostic agents for SPECT. In order to compare these two amino acid analogues and to examine

  12. I-123/125-labelled 2-iodo-L-phenylalanine and 2-iodo-D-phenylalanine: comparative uptake in various tumour types and biodistribution in mice

    NARCIS (Netherlands)

    Kersemans, V.; Cornelissen, B.; Kersemans, K.; Bauwens, M.; Dierckx, R.A.; De Spiegeleer, B.; Mertens, J.; Slegers, G.

    2006-01-01

    Purpose: In vitro in the R1M cell model and in vivo in the R1M tumour-bearing athymic model, both [I-123]- 2- iodo-L-phenylalanine and [I-123]-2-iodo-D-phenylalanine have shown promising results as tumour diagnostic agents for SPECT. In order to compare these two amino acid analogues and to examine

  13. Adrenocortical Carcinoma: False Positive in an I-123 Metaiodobenzylguanidine Scan

    Energy Technology Data Exchange (ETDEWEB)

    Rey, Cristina Rodriguez; Candil, Aida Ortega; Galvan, Eliseo Vano; Martin, Maria Nieves Cabrera; Delgado, Jose Luis Carreras [Clinico San Carlos Hospital, Madrid (Spain)

    2016-06-15

    A 55-year-old man with a personal his history of left pheochromocytoma 2 years previously presented with an abdominal ultrasound showed a large left upper quadrant solid mass (calipers), with heterogeneous echogenicity and central cystic degeneration areas or necrosis. F-18 Fluorodeoxyglucose (FDG) positron emission tomography (PET)/CT was performed as ACC was suspected. ACC is an uncommon malignant neoplasm of unknown cause; however, smoking and oral contraceptives may be risk factors. Patients usually present with advanced-stage disease and have poor prognosis, with a 2-year recurrence rate that ranges form 73 to 86 %. An MIBG scan was performed after injection of 185 MBq of I-123 MIBG intravenously. Single photon emission tomography (SPECT)/CT scan showed abnormal isotope accumulation in the tumor region. The patient underwent total resection of the tumor. The histology was of ACC, with areas of necrosis and cystic areas, so the result of the MIBG scan was a false positive. Authors divided false-positive MIBG studies into three categories. The first category is neuroendocrine lesions other than pheochromocytomas (tumors of the APUD series). the second category consists in adrenal lesions other than pheochromocytomas (adenomas, ACC) the reasons for this abnormal uptake are unclear. The third category consists of tracer uptake adjacent to the adrenal due to abnormalities in the route of excretion.

  14. Interest of a systemic SPECT/CT during scintigraphy with Mibg-{sup 123}I: about one case of pheochromocytoma; Interet d'une TEMP/TDM systematique lors de la scintigraphie a la MIBG-I123: a propos d'un cas de pheochromocytome

    Energy Technology Data Exchange (ETDEWEB)

    Hassler, S.; Bourahla, K.; Schneegans, O. [CLCC Paul-Strauss, 67 - Strasbourg (France)

    2010-07-01

    The use of SPECT/CT in the abdomen and pelvis for a scintigraphy with {sup 123}I -Mibg for diagnosis of pheochromocytoma is still dependent on local practice. We plead for a systematic SPECT/CT and illustrate this position by a clinical case. Conclusions: The SPECT/CT improves the performance of scintigraphy with {sup 123}I Mibg as well in sensitivity towards deep sources as in specificity for sources badly defined in planar imaging. We plead for a systematic use of SPECT/CT when clinical indication is well supported. (N.C.)

  15. Value of I-123-subtraction and single-photon emission computed tomography in addition to planar Tc-99m-MIBI scintigraphy before parathyroid surgery

    NARCIS (Netherlands)

    Jorna, Francisca H.; Jager, Pieter L.; Que, Tjin H.; Lemstra, Clara; Plukker, John T. M.

    2007-01-01

    Purpose. To find out if single-photon emission computed tomography (SPECT) and I-123-subtraction can enhance the findings of Tc-99-methoxyisobutylisonitrile (MIBI) scintigraphy for the preoperative localization of parathyroid (PT) tumors. Methods. Among the 111 consecutive patients who underwent pre

  16. Single photon emission computed tomography (SPECT) findings using N-isopropyl-p-[{sup 123}I]iodoamphetamine ({sup 123}I-IMP) in schizophrenia and atypical psychosis

    Energy Technology Data Exchange (ETDEWEB)

    Suga, Hidemichi; Hayashi, Takuji; Mitsugi, Ohara [Aichi Medical Univ., Nagakute (Japan)

    1994-12-01

    As a basis for possible classification of schinzophrenic psychoses into schizophrenia and atypical psychosis, we studied the brain functional differences among 16 schizophrenic patients, 16 atypical psychosis patients and 16 healthy volunteers by single photon emission computed tomography (SPECT) using N-isopropyl-p-[{sup 123}I] iodoamphetamine. As a result, schizophrenics showed hypofrontality. On the other hand, atypical psychotics had no such hypofrontality but showed a reduced uptake rate in the right thalamic region. No influence of sex, duration of illness and medication was confirmed by the findings. The results suggest that schizophrenics might have some lesions in the frontal regions, whereas atypical psychotics might have no such lesions, but dysfunction in the right thalamic region. Consequently, the SPECT findings as least indicate possibly different etiologies for schizophrenia and atypical psychosis. (author).

  17. Iofetamine hydrochloride I 123: a new radiopharmaceutical for cerebral perfusion imaging

    Energy Technology Data Exchange (ETDEWEB)

    Druckenbrod, R.W.; Williams, C.C.; Gelfand, M.J.

    1989-01-01

    Iofetamine hydrochloride I-123 permits cerebral blood perfusion imaging with single photon emission computed tomography (SPECT). SPECT is more widely available than positron emission tomography, and complements anatomic visualization with X-ray computed tomography (CT) or magnetic resonance imaging. Iofetamine is an amphetamine analog that is rapidly taken up by the lungs, then redistributed principally to the liver and brain. The precise mechanism of localization has not been determined, but is believed to result from nonspecific receptor binding. Brain uptake peaks at 30 minutes postinjection and remains relatively constant through 60 minutes. The drug is metabolized and excreted in the urine, with negligible activity remaining at 48 hours. When compared with CT in stroke patients, visualization may be performed sooner after symptom onset and a larger zone of involvement may be evident with iofetamine. Localization of seizure foci and diagnosis of Alzheimer's disease may also be possible. As CT has revolutionized noninvasive imaging of brain anatomy, SPECT with iofetamine permits routine cerebral blood flow imaging. 36 references.

  18. Redistribution in I-123 N-isopropyl-p iodoamphetamine single-photon emission computed tomography in cerebrovascular disease and the effects of rehabilitation

    Energy Technology Data Exchange (ETDEWEB)

    Fukumitsu, N.; Kawakami, G.; Uchiyama, M.; Mori, Y. [Dept. of Radiology, Jikei University School of Medicine, Tokyo (Japan); Ogi, S. [Dept. of Radiology, Ichikawa Hospital of Tokyo Dental University, Tokyo (Japan); Takehara, I.; Miyano, S. [Dept. of Rehabilitation Medicine, Jikei Daisan Hospital (Japan); Katagiri, N. [Dept. of Rehabilitation Medicine, Kanagawa Rehabilitation Centre (Japan)

    2001-06-01

    We performed N-isopropyl-p (I-123) iodoamphetamine (IMP) single-photon emission computed tomography (SPECT) on 28 patients with severe cerebrovascular disease before rehabilitation, and compared the degree of redistribution and the assessment of activities of daily living (ADL). We calculated a redistribution (RD) ratio in the central and peripheral parts of the lesions: RD ratio (c) and RD ratio (p). We classified the patients into four groups based on the degree of redistribution: complete: both RD ratio (c) and (p) {>=} 75; peripheral: RD ratio (c) < 75, RD ratio (p) {>=} 75; incomplete: both RD ratio (c) and (p) < 75 and at least one of RD ratio (c) or (p) {>=} 25; no redistribution: both RD ratio (c) and (p) < 25. We assessed the ADL using the modified Barthel index (BI). {delta}BI was defined as BI after rehabilitation-BI before rehabilitation (BIpost-BIpre). The {delta}BI of the four groups were as follows: complete-redistribution group (40.8 {+-} 22.8), peripheral-redistribution group (40.0 {+-} 15.8), incomplete-redistribution group (27.2 {+-} 22.6), no-redistribution group (8.8 {+-} 12.3). The {delta}BI of the complete and peripheral redistribution groups were significantly higher than that of the no-redistribution group. However, {delta}BI was almost the same in the complete- and peripheral-redistribution groups. This suggests that the effect of rehabilitation might be closely related to the viability of the peripheral part of the lesion. (orig.)

  19. Localization of a pheochromocytoma using I-123 MIBG adrenal scintigraphy.

    Directory of Open Access Journals (Sweden)

    Shirkare S

    1994-04-01

    Full Text Available In a patient with the clinical diagnosis of pheochromocytoma, the localization of the tumor is essential for planning treatment. Recently, we have performed I-123 metaiodobenzylguanidine (MIBG adrenal scintigraphy in a patient presenting with a history of paroxysmal hypertension. Scintigraphy accurately located an ectopic unilateral pheochromocytoma. The scintigraphic diagnosis was confirmed by surgery and a diagnosis of ectopic unilateral pheochromocytoma was made by histopathological examination. This case report illustrates the specific diagnosis of pheochromocytoma by I-123 MIBG scintigraphy which is especially useful when other diagnostic procedures are equivocal.

  20. Improved Benefit of SPECT/CT Compared to SPECT Alone for the Accurate Localization of Endocrine and Neuroendocrine Tumors

    Directory of Open Access Journals (Sweden)

    Gonca G. Bural

    2012-12-01

    Full Text Available Objective: To assess the clinical utility of SPECT/ CT in subjects with endocrine and neuroendocrine tumors compared to SPECT alone. Material and Methods: 48 subjects (31 women;17 men; mean age 54±11 with clinical suspicion or diagnosis of endocrine and neuroendocrine tumor had 50 SPECT/CT scans (32 Tc-99m MIBI, 5 post treatment I-131, 8 In-111 Pentetreotide, and 5 I-123 MIBG. SPECT alone findings were compared to SPECT/CT and to pathology or radiological follow up. Results: From the 32 Tc-99m MIBI scans, SPECT accurately localized the lesion in 22 positive subjects while SPECT/CT did in 31 subjects. Parathyroid lesions not seen on SPECT alone were smaller than 10 mm. In five post treatment I-131 scans, SPECT alone neither characterized, nor localized any lesions accurately. SPECT/CT revealed 3 benign etiologies, a metastatic lymph node, and one equivocal lesion. In 8 In-111 Pentetreotide scans, SPECT alone could not localize primary or metastatic lesions in 6 subjects all of which were localized with SPECT/CT. In five I-123 MIBG scans, SPECT alone could not detect a 1.1 cm adrenal lesion or correctly characterize normal physiologic adrenal uptake in consecutive scans of the same patient with prior history of adrenelectomy, all of which were correctly localized and characterized with SPECT/CT. Conclusion: SPECT/CT is superior to SPECT alone in the assessment of endocrine and neuroendocrine tumors. It is better in lesion localization and lesion characterization leading to a decrease in the number of equivocal findings. SPECT/CT should be included in the clinical work up of all patients with diagnosis or suspicion of endocrine and neuroendocrine tumors. (MIRT 2012;21:91-96

  1. Synthesis and biological evaluation of I-125/I-123-labelled analogues of citalopram and escitalopram as potential radioligands for imaging of the serotonin transporter

    DEFF Research Database (Denmark)

    Madsen, Jacob; Elfving, Betina; Frokjaer, Vibe G.

    2011-01-01

    Two novel radioligands for the serotonin transporter (SERT), [I-125]{3-[5-iodo-1-(4-fluorophenyl)-1,3-dihydroisobenzofuran-1-yl]-propyl}-dimethylamine ([I-125]-2) and S-[I-125]{3-[5-iodo-1-(4-fluorophenyl)-1,3-dihydroisobenzofuran-1-yl]-propyl}-dimethylamine ([I-125]-(S)-2) were synthesized in a ...... of the radioligand in imaging cortical SERT distribution in vivo. These data suggest that the iodine-labelled derivatives of citalopram and escitalopram are not superior to another SPECT tracer for the SERT, namely [I-123] ADAM....

  2. Scintigraphic assessment of cardiac sympathetic innervation with I-123-metaiodobenzylguanidine in cardiomyopathy. Special reference to cardiac arrhythmia

    Energy Technology Data Exchange (ETDEWEB)

    Asano, Takahisa; Otsuka, Nobuaki; Sone, Teruki; Mimura, Hiroaki; Yanagimoto, Shinichi; Tomomitsu, Tatsushi; Fukunaga, Masao [Kawasaki Medical School, Kurashiki, Okayama (Japan); Morita, Koichi

    1999-07-01

    Cardiac sympathetic imagings with I-123-metaiodobenzylguanidine (MIBG) were carried out in 5 cases with dilated cardiomyopathy (DCM), 26 cases with hypertrophic cardiomyopathy (HCM), and 4 cases without cardiac disease as a control to assess cardiac sympathetic innervation qualitatively and quantitatively, and to clarify the relation of MIBG accumulation to arrhythmia. MIBG scintigraphy was performed at 15 min. (early image) and 4 hr. (delayed image) after intravenous injection of MIBG 111 MBq. The MIBG uptake ratio of mediastinum (H/M) and the cardiac washout rate (WR) from early to delayed images were calculated. On both early and delayed SPECTs, MIBG uptake was assessed by defect scores (DSs). Regarding the cases with HCM, the MIBG uptake ratio, WR, and DS were also compared in cases with and without arrhythmia. In DCM, the MIBG uptake on delayed SPECT was markedly low, the H/M ratio was significantly lower, and the DS was significantly higher than in the control (all p<0.05). As for the WR, there was no significant difference between HCM, DCM and the control. In HCM, significantly reduced MIBG uptake was observed in cases with ventricular techycardia (VT) and in cases with atrial fibrillation (Af), as compared with cases without arrhythmia (all p<0.05). There results suggest that MIBG scintigraphy might be a useful tool in the assessment of cardiac sympathetic abnormalities in cardiomyopathy, especially in cases with arrhythmia. (author)

  3. I-123 MIBG imaging and intraoperative localization of metastatic pheochromocytoma: a case report

    DEFF Research Database (Denmark)

    Buhl, Thora; Mortensen, Jann; Kjaer, Andreas

    2002-01-01

    The authors describe the diagnostic use of I-123 MIBG scintigraphy in a 61-year-old man who was thought to have a recurrence 25 years after a left adrenalectomy for a pheochromocytoma. Preoperative I-123 MIBG scintigraphy was performed twice along with intraoperative gamma probe localization...

  4. IMP-8 Magnetometer Experiment

    Science.gov (United States)

    Szabo, Adam

    2011-01-01

    IMP-8 (IMP-J) was launched by NASA on October 26, 1973 to measure the magnetic fields, plasmas, and energetic charged particles (e.g., cosmic rays) of the Earth's magnetotail and magnetosheath and of the near-Earth solar wind. IMP-8, the last often IMP (Interplanetary Monitoring Platform) or AIMP (Anchored-IMP) spacecraft launched in 10 years, operated until October 7, 2006, in a near-circular, 35 Earth Radii, 12-day orbit. It was an important adjunct to the International Solar Terrestrial Physics program, provided in-ecliptic, one Astronomical Unit baseline data for the deep space Pioneer, Voyager and Ulysses missions, and built a long-time-series database useful in understanding long-term solar processes .

  5. Slowly progressive fluent aphasia; Clinical features and an imaging study including MRI, SPECT and PET

    Energy Technology Data Exchange (ETDEWEB)

    Sakurai, Yasuhisa; Momose, Toshimitsu; Watanabe, Toshiaki; Ishikawa, Takashi; Iwata, Makoto (Tokyo Univ. (Japan). Faculty of Medicine); Bando, Mitsuaki

    1991-05-01

    Three patients with slowly progressive fluent aphasia are reported. One of the patients presented with memory disturbance. They were characterized clinically by having selective deficits in vocabulary, which resulted in impairment of confrontation naming, and auditory comprehension. MRI showed an atrophy not only in the left temporal lobe (including the superior, middle and inferior temporal gyri), hippocampus, parahippocampual gyrus, and fusiform gyrus, but also in the left parietal lobe. I-123 IMP SPECT and F-18 FDG PET were used to determine regional cerebral blood flow and regional cerebral metabolic rate, respectively. In addition to the decreased tracer uptake in the left temporal and/or parietal lobe, a decreased uptake was seen in the bilateral basal ganglia, the inner side of the temporal lobe (including the bilateral hippocampus), the right anterior temporal lobe, and the left thalamus. These findings may deny the previous thought that lesions are localized in slowly progressive fluent aphasia. Furthermore, noticeable difficulty in naming, i.e., patients unable to recognize the right answer, are considered attributable to widespread lesions from the whole left temporal lobe, including the hippocampus, to the right temporal lobe. (N.K.).

  6. Development of target system for production of I-123 in Cyclotron 30

    Energy Technology Data Exchange (ETDEWEB)

    You, Jae Jun; Jung, Hyun Woo; Kim, Byung Il and others

    2013-10-15

    This work was mainly focused on the development of target system for production of I-123 in Cyclotron 30. We have analyzed the original I-123 target system which is constructed by company in Canada and designed with solid works 3D CAD program. We have designed newly by changing cooling method of straight line into spiral line. We look forward to increase the cooling efficiency by decreasing water resistance inside the target chamber. The target system also is made by modular method which is possible to change target quickly. So, the radiation exposure to engineers when exchanging target will be minimized. Only KIRAMS can produce I-123 radiopharmaceuticals in Korea. So we need to acquire techniques not only supplementing drawbacks of the existing target, but also minimizing the radiation exposure during maintenance of target. The new water cooling system will hold the pressure of the target constantly. This will enhance the yield of I-123. The first development of Xe-124 target in Korea will result in more distributed environment of I-123 to diagnose thyroid gland cancer.

  7. Comparison of brain serotonin transporter using [I-123]-ADAM between obese and non-obese young adults without an eating disorder

    Science.gov (United States)

    Wu, Chih-Hsing; Chang, Chin-Sung; Yang, Yen Kuang; Shen, Lie-Hang; Yao, Wei-Jen

    2017-01-01

    Cerebral serotonin metabolism has an important but controversial role in obesity. However, it is not given enough attention in morbidly obese young adults. We used single photon emission computed tomography (SPECT) with [I-123]-labeled 2-((2-((dimethylamino)methyl)phenyl)thio)-5-iodophenylamine (ADAM) to investigate changes in serotonin transporter (SERT) availability in 10 morbidly obese young adults without an eating disorder (M/F = 5/5, body mass index (BMI): 40.3 ± 4.1 kg/m2, percentage of body fat (BF%): 46.0 ± 3.9%) and 10 age- and sex-matched non-obese controls (BMI: 20.3 ± 1.2 kg/m2, BF%: 20.6 ± 8.9%). All participants underwent SPECT at 10 min and 6 h after an injection of 200 MBq of [I-123]-ADAM. The SERT binding site (midbrain) was drawn with cerebellum normalization. The BF% and fat distribution were measured using dual-energy X-ray absorptiometry. The midbrain/cerebellum SERT binding ratios (2.49 ± 0.46 vs. 2.47 ± 0.47; p = 0.912) at 6 h were not significantly different between groups, nor was the distribution of the summed images at 10 min (1.36 ± 0.14 vs. 1.35 ± 0.11; p = 0.853). There were no significant correlations between midbrain/cerebellum SERT binding ratio and age, BMI, BF%, or fat distribution. No significant difference in SERT availability in the midbrain between morbidly obese and non-obese young adults without an eating disorder indicates an unmet need for investigating the role of cerebral serotonin in obesity. PMID:28182708

  8. SPECT/CT imaging in children with papillary thyroid carcinoma

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Hwa-Young; Gelfand, Michael J.; Sharp, Susan E. [Cincinnati Children' s Hospital, Department of Radiology, Cincinnati, OH (United States)

    2011-08-15

    SPECT/CT improves localization of single photon-emitting radiopharmaceuticals. To determine the utility of SPECT/CT in children with papillary thyroid carcinoma. 20 SPECT/CT and planar studies were reviewed in 13 children with papillary thyroid carcinoma after total thyroidectomy. Seven studies used I-123 and 13 used I-131, after elevating TSH by T4 deprivation or intramuscular thyrotropin alfa. Eight children had one study and five children had two to four studies. Studies were performed at initial post-total thyroidectomy evaluation, follow-up and after I-131 treatment doses. SPECT/CT was performed with a diagnostic-quality CT unit in 13 studies and a localization-only CT unit in 7. Stimulated thyroglobulin was measured (except in 2 cases with anti-thyroglobulin antibodies). In 13 studies, neck activity was present but poorly localized on planar imaging; all foci of uptake were precisely localized by SPECT/CT. Two additional foci of neck uptake were found on SPECT/CT. SPECT/CT differentiated high neck uptake from facial activity. In six studies (four children), neck uptake was identified as benign by SPECT/CT (three thyroglossal duct remnants, one skin contamination, two by precise anatomical CT localization). In two children, SPECT/CT supported a decision not to treat with I-131. When SPECT/CT was unable to identify focal uptake as benign, stimulated thyroglobulin measurements were valuable. In three of 13 studies with neck uptake, SPECT/CT provided no useful additional information. SPECT/CT precisely localizes neck iodine uptake. In small numbers of patients, treatment is affected. SPECT/CT should be used when available in thyroid carcinoma patients. (orig.)

  9. SPECT/CT in pediatric patient management

    Energy Technology Data Exchange (ETDEWEB)

    Nadel, Helen R. [British Columbia Children' s Hospital, University of British Columbia, Pediatric Radiologist and Nuclear Medicine Physician, Division of Nuclear Medicine Department of Radiology, Vancouver, British Columbia (Canada)

    2014-05-15

    Hybrid SPECT/CT imaging is becoming the standard of care in pediatric imaging. Indications are mainly for oncologic imaging including mIBG scintigraphy for neuroblastoma and I-123 post surgical imaging of children with thyroid carcinoma, bone scintigraphy for back pain, children referred from sports medicine and neurodevelopmentally delayed children presenting with pain symptoms. The studies provide improved diagnostic accuracy, and oncologic imaging that includes optimized CT as part of the SPECT/CT study may decrease the number of studies and sedation procedures an individual child may need. The studies, however, must be tailored on an individual basis as the addition of the CT study can increase exposure to the child and should only be performed after appropriate justification and with adherence to optimized low dose pediatric protocols. (orig.)

  10. NONINVASIVE MEASUREMENT OF INTRARENAL BLOOD-FLOW DISTRIBUTION - KINETIC-MODEL OF RENAL I-123 HIPPURAN HANDLING

    NARCIS (Netherlands)

    JANSSEN, WMT; BEEKHUIS, H; DEBRUIN, R; DEJONG, PE; DEZEEUW, D

    1995-01-01

    A new technique for noninvasive measurement of intrarenal blood flow distribution over cortex and medulla is proposed. The tech nique involves analysis of I-123-labeled hippuran renography, according to a kinetic model that describes the flow of I-123- hippuran from the heart (input) through the ren

  11. Quantitative {sup 123}I IMP and {sup 99m}Tc HMPAO imaging in the dog following cocaine administration

    Energy Technology Data Exchange (ETDEWEB)

    Susskind, Herbert; Weber, David A.; Ivanovic, Marija; Wong, Christopher T.C.; Haan, Constance E. de; Gavin, Patrick R

    1996-04-01

    SPECT and associated imaging procedures were used in beagle dogs to evaluate the uptake, distribution, and clearance properties of i.v.-injected {sup 123}I IMP (IMP) and {sup 99m}Tc HMPAO (HMPAO) in the brain, lungs, liver, and kidneys; (2) quantify the acute effects (after 15 sec) of very low doses (0.5 or 1.0 mg/kg) cocaine on the kinetics and localization properties of IMP and HMPAO; and (3) evaluate comparative imaging properties of IMP and HMPAO for measuring regional cerebral blood flow (rCBF). Regional and global uptake and localization of IMP or HMPAO were evaluated in control studies using dynamic planar (0-30 min) and SPECT imaging (at 35 min). The regional distribution properties of IMP and HMPAO in the brain were estimated from regions of interest (ROIs) drawn around anatomic structures on MR slices and manually registered with corresponding SPECT slices. Cocaine significantly reduced the 30-min IMP uptake in the brain and lungs by {approx}15%, but only slightly changed HMPAO uptake in the brain and other organs. In the control studies, the respective uptakes of IMP in the brain and lungs were 9 and 39% greater (p < 0.01) than those of HMPAO. In control SPECT studies, the highest uptake of IMP was observed in the thalamus and progressively less activity was observed in the parietal lobe, frontal lobe, cerebellum, occipital lobe, and entire brain; activity in the olfactory bulb was lower than in all other regions. Cocaine reduced IMP uptake in the cerebellum (p < 0.01), occipital lobe (p < 0.01), and entire brain (p < 0.05). IMP uptake (cpm/pixel-mCi) in the different brain regions was 1.3 to 2.1 times greater than that of HMPAO (p < 0.001). HMPAO uptake was more homogeneous throughout the gray matter of the brain; no significant uptake differences were observed among flagged regions. Results indicate that single, acute doses of cocaine, 0.5 and 1.0 mg/kg, significantly altered the uptake and localization properties of IMP in the dog's brain, lungs

  12. Cardiac I-123 MIBG scintigraphy in differentiation of Parkinson's disease and multiple system atrophy

    Energy Technology Data Exchange (ETDEWEB)

    Chung, Hyun Woo; Lee, Kyung Han; Chung, Eun Joo; Lee, Eun Jeong; Choi, Joon Young; Choi, Yong; Choe, Yeam Seong; Lee, Won Yong; Km, Byung Tae [Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul (Korea, Republic of)

    2005-07-01

    In idiopathic Parkinson's disease (PD) postganglionic involvement of the autonomic nervous system predominates, whereas in multiple system atrophy (MSA) preganglionic structures are mainly affected. Since cardiac I-123 metaiodobenzylguanidine (MIBG) scintigraphy can assess functional integrity of postganglionic sympathetic neurons, we evaluated its ability to differentiate PD and MSA. Cardiac MIBG scintigraphy was performed on 47 patients (26 PD and 21 MSA; 25 males, 62{+-}9 yr) and 8 normal controls (NC: 3 males, 45{+-}9 yr). All patients were excluded for myocardial ischemia by TI-201 SPECT. MSA patients were predominantly parkinsonism (MSA-p) and cerebellar subtypes (MSA-c) in 11 and 10 cases, respectively. Early (20 min) and delayed (4 hr) images were used to obtain heart/mediastinum count (H/M) ratios and washout rates (WRs). The both early (E) and delayed (D) H/M ratios were significantly decreased in PD (E; 1.54{+-}0.23, D: 1.36{+-}0.24) as well as MSA (E; 1.80{+-}0.44, D; 1.88{+-}0.51) patients compared to NC (E; 2.74{+-}0.97, D; 2.94{+-}1.20) (all p<0.01). The PD group had a significantly lower H/M ratio than the MSA group (E; p = 0.03, D; p = 0.003). The WRs of the PD patients (59.5{+-}15.4%) were significantly increased compared to MSA patients and NC (26.6{+-}23.9% and 19.7{+-}11.5%, both p<0.0001). The WRs between MSA patients and NC were not significantly different. In the differential diagnosis of the PD and MSA, the sensitivity, specificity and accuracy by early H/M ratio (cut-off = 1.80) were 84.6% (22/26), 52.5% (11/21), and 70.2% (33/47), respectively, by delayed H/M ratio, 100.0% (26/26), 66.7% (14/21), and 85.1% (40/47), respectively, and by WR (cut-off 40.0%), 92.3% (24/26), 76.2% (16/21), and 85.1% (40/47), respectively. While the delayed H/M ratios were below 1.80 in 5/11 MSA-p patients, 1 of those 5 MSA-p patients had a decreased WR. Cardiac MIBG scintigraphy was useful for differentiation of PD from MSA and NC by delayed H/M ratio

  13. Accuracy of quantitative reconstructions in SPECT/CT imaging

    Energy Technology Data Exchange (ETDEWEB)

    Shcherbinin, S; Celler, A [Department of Radiology, University of British Columbia, 366-828 West 10th Avenue, Vancouver BC, V5Z 1L8 (Canada); Belhocine, T; Vanderwerf, R; Driedger, A [Department of Nuclear Medicine, London Health Sciences Centre, 375 South Street, PO Box 5375, London ON, N6A 4G5 (Canada)], E-mail: shcher2@interchange.ubc.ca

    2008-09-07

    The goal of this study was to determine the quantitative accuracy of our OSEM-APDI reconstruction method based on SPECT/CT imaging for Tc-99m, In-111, I-123, and I-131 isotopes. Phantom studies were performed on a SPECT/low-dose multislice CT system (Infinia-Hawkeye-4 slice, GE Healthcare) using clinical acquisition protocols. Two radioactive sources were centrally and peripherally placed inside an anthropometric Thorax phantom filled with non-radioactive water. Corrections for attenuation, scatter, collimator blurring and collimator septal penetration were applied and their contribution to the overall accuracy of the reconstruction was evaluated. Reconstruction with the most comprehensive set of corrections resulted in activity estimation with error levels of 3-5% for all the isotopes.

  14. Diagnostic performance and prognostic value of extravascular retention of I-123-labeled serum amyloid P component in systemic amyloidosis

    NARCIS (Netherlands)

    Hazenberg, Bouke P. C.; van Rijswijk, Martin H.; Lub-de Hooge, Marjolijn N.; Vellenga, Edo; Haagsma, Elizabeth B.; Posthumus, Marcel D.; Jager, Pieter L.

    2007-01-01

    Serum amyloid P component (SAP) binds to amyloid.I-123-SAP scintigraphy is used to evaluate the extent and distribution of amyloid in systemic amyloidosis and has great clinical value in the detection of systemic amyloidosis. The aim of the study was to assess during scintigraphy the diagnostic perf

  15. N-isopropyl-p-( sup 123 I)iodoamphetamine SPECT in MELAS syndrome: Comparison with CT and MR imaging

    Energy Technology Data Exchange (ETDEWEB)

    Satoh, M.; Ishikawa, N.; Yoshizawa, T.; Takeda, T.; Akisada, M. (Univ. of Tsukuba (Japan))

    1991-01-01

    Regional cerebral perfusion was studied in three patients with the mitochondrial encephalomyopathy with lactic acidosis and stroke-like episodes (MELAS) syndrome, using single photon emission computed tomography (SPECT) with N-isopropyl-p-(123I)iodoamphetamine (IMP). Accumulation of the tracer was relatively decreased in the parietooccipital regions and also in the frontotemporal regions after stroke-like episodes. However, quantitative regional cerebral blood flow (rCBF) measurement showed that rCBF was relatively well preserved even at these sites, and a hyperemic state was observed at the sites of normal accumulation. IMP SPECT may be useful in the diagnosis and assessment of the progress of the MELAS syndrome.

  16. Individual-Reader Diagnostic Performance and Between-Reader Agreement in Assessment of Subjects with Parkinsonian Syndrome or Dementia Using I-123-Ioflupane Injection (DaTscan) Imaging

    NARCIS (Netherlands)

    Seibyl, John P.; Kupsch, Andreas; Booij, Jan; Grosset, Donald G.; Costa, Durval C.; Hauser, Robert A.; Darcourt, Jacques; Bajaj, Nin; Walker, Zuzana; Marek, Kenneth; McKeith, Ian; O'Brien, John T.; Tatsch, Klaus; Tolosa, Eduardo; Dierckx, Rudi A.; Grachev, Igor D.

    2014-01-01

    Establishing an early, accurate diagnosis is fundamental for appropriate clinical management of patients with movement disorders or dementia. Ioflupane I-123 Injection (DaTscan, I-123-ioflupane) is an important adjunct to support the clinical diagnosis. Understanding individual-reader diagnostic per

  17. Brain SPECT. SPECT in der Gehirndiagnostik

    Energy Technology Data Exchange (ETDEWEB)

    Feistel, H. (Erlangen-Nuernberg Univ., Erlangen (Germany). Nuklearmedizinische Klinik mit Poliklinik)

    1991-12-01

    Brain SPECT investigations have gained broad acceptance since the introduction of the lipophilic tracer Tc-99m-HMPAO. Depending on equipment and objectives in different departments, the examinations can be divided into three groups: 1. Under normal conditions and standardised patient preparation the 'rest' SPECT can be performed in every department with a tomographic camera. In cerebrovascular disease there is a demand for determination of either the perfusion reserve in reversible ischemia or prognostic values in completed stroke. In cases of dementia, SPECT may yield useful results according to differential diagnosis. Central cerebral system involvement in immunologic disease may be estimated with higher sensitivity than in conventional brain imaging procedures. In psychiatric diseases there is only a relative indication for brain SPECT, since results during recent years have been contradictory and may be derived only in interventional manner. In brain tumor diagnostics SPECT with Tl-201 possibly permits grading. In inflammatory disease, especially in viral encephalitis, SPECT may be used to obtain early diagnosis. Normal pressure hydrocephalus can be distinguished from other forms of dementia and, consequently, the necessity for shunting surgery can be recognised. 2. In departments equipped for emergency cases an 'acute' SPECT can be performed in illnesses with rapid changing symptoms such as different forms of migraine, transient global amnesia, epileptic seizures (so-called 'ictal SPECT') or urgent forms like trauma. 3. In cooperation with several departments brain SPECT can be practised as an interventional procedure in clinical and in scientific studies. (orig./MG).

  18. Rapid discrimination of blaIMP-1, blaIMP-6, and blaIMP-34 using a multiplex PCR.

    Science.gov (United States)

    Kayama, Shizuo; Ohge, Hiroki; Sugai, Motoyuki

    2017-04-01

    Stealth-type carbapenem-resistant Enterobacteriaceae that are resistant to almost all β-lactams except imipenem is emerging in Japan. This resistance is mediated by specific variants of the metallo-β-lactamases (blaIMP-6 or blaIMP-34) that differs by one amino acid from the common variant blaIMP-1. We developed an amplification refractory mutation system (ARMS) PCR assay enabling rapid, sequence independent, identification of these variants.

  19. SPECT in psychiatry. SPECT in der Psychiatrie

    Energy Technology Data Exchange (ETDEWEB)

    Barocka, A. (Psychiatrische Klinik und Poliklinik, Erlangen (Germany)); Feistel, H. (Nuklearmedizinische Klinik, Erlangen (Germany)); Ebert, D. (Psychiatrische Klinik und Poliklinik, Erlangen (Germany)); Lungershausen, E. (Psychiatrische Klinik und Poliklinik, Erlangen (Germany))

    1993-08-13

    This review presents Single Photon Emission Computed Tomography (SPECT) as a powerful tool for clinical use and research in psychiatry. Its focus is on regional cerebral blood flow, measured with technetium labelled HMPAO. In addition, first results with brain receptor imaging, concerning dopamin-D[sub 2] and benzodiazepine receptors, are covered. Due to major improvements in image quality, and impressive number of results has been accumulated in the past three years. The authors caution against using SPECT results as markers for disease entities. A finding like 'hypofrontality' is considered typical of a variety of mental disorders. Clearly both, more experience with SPECT and contributions from psychopathology, are needed. (orig.)

  20. Preclinical testing of F-18 and I-123 radioligands involving autoradiography and a phosphor-imaging device

    Energy Technology Data Exchange (ETDEWEB)

    Gatley, S.J.; Pyatt, B.; Gifford, A.N. [Brookhaven National Lab., Upton, NY (United States)] [and others

    1997-05-01

    During the development and validation of a receptor-binding radiopharmaceutical it is necessary to assess its brain regional distribution. Film contact autoradiography (AR) points such distributions to be evaluated on a fine anatomical scale, and avoids the inherent risk a microdissection experiments of missing interesting localization pattern. However, in vivo film AR may require a 1000-fold higher activity of a F-18 or I-123 radioligand than microdissection. In addition to health physics considerations, problems with receptor saturation can easily occur after injecting, say, 10 mCi into a 250g rat. A less commonly appreciated problem even with in vitro AR, using slide-mounted brain sections, is that it may be impossible to use sufficient F-18 to obtain acceptable images using film, because of receptor saturation. Phosphorimaging is superior in sensitivity to film by a factor of 10-50, and so relaxes constraints on injected activities and specific activities. Furthermore, it is also superior to film in terms of dynamic range and linearity of response. We have recently used phosphorimaging in two situations where film AR gave unsatisfactory results. (1) the novel cannabinoid CB1 receptor radioligand, I-123 AM281, was injected intravenously at 800 microcurie per rat. Animals were sacrificed at 2 h, frozen and whole body sections prepared. I-123 was clearly visualized in discrete brain areas (substantia nigra, globus pallidus, entopeduncular nucleus , hippocampus and cerebellum) consistent with the distribution of CB1 receptors previously demonstrated only in in vitro studies. (2) Rat brain sections were thaw mounted on gelatin/alum coated glass and incubated with the novel nicotinic acetylcholine receptor ligand F-18 2{prime}-fluoro-2{prime} nor-chloroepibatidine (1 nM).

  1. Características clínicas, neurofisiológicas y de imagen con SPECT I123-FP-CIT en el parkinsonismo vascular

    OpenAIRE

    Benítez Rivero, Sonia

    2015-01-01

    La definición actual de parkinsonismo vascular (PV) es la aparición de signos parkinsonianos (definido por la presencia de bradicinesia más uno de los siguientes: rigidez, temblor, inestabilidad postural) desarrollados como consecuencia de lesiones vasculares, en ausencia de confirmación histopatológica de enfermedad de Parkinson (EP). El PV es una patología en la que en la literatura se ha descrito gran variabilidad en sintomatología y respuesta a tratamiento. Sin embargo, resulta de gran im...

  2. Syntheses and biological evaluation of F-18 and I-123 labeled porphyrins as potential tumor imaging agents

    Energy Technology Data Exchange (ETDEWEB)

    Lee, J. H.; Ji, D. Y. [Inha University, Incheon (Korea, Republic of); Moon, B. S.; Lee, T. S.; Lee, D. H.; Lee, K. C.; Ahn, G. I.; Yang, S. D.; Choi, C. W.; Jun, K. S. [KIRAMS, Seoul (Korea, Republic of)

    2005-07-01

    Photofrin has currently been approved for general use by licensing authorities to treatment for solid tumor and cancer using photodynamic therapy (PDT) that treat to photochemical effect induced by light. Recently, meso-tetra(3-hydroxyphenyl)porphyrin has been developed as one of best tumor localizer and also shown a favorable tissue distribution. We have studied to develop I-123 labeled meso-tetra(3-methoxyphenyl)porphyrins for tumor imaging. We have studied to develop iodine-123 labeled meso-tetra(3-carboxymethoxy phenyl)porphyrin for tumor imaging agent. The radioiodinated porphyrin compound was obtained by the iodination reaction of tin precursor (50 ig) of porphyrin with Na-123I (200 {mu}L, 100-200 mCi), in the presence of peracetic acid (40 {mu}L) in ethanol. Iodine-123 labeled porphyrin derivative was obtained in 20-30% radiochemical yield and purified by HPLC at 2 mL/min using EtOH/water gradient condition and the fraction at 24-26 min was collected and characterized to desired compound by co injection with cold porphyrin analogue. Total time was around 120 min. The in vitro and in vivo of I-123 labeled porphyrin derivative is under studying.

  3. Diminished response to furosemide in I-123 Hippuran renal studies of renovascular hypertension caused by unilateral renal artery stenosis

    Energy Technology Data Exchange (ETDEWEB)

    Flueckiger, F.M.; Fueger, G.F.; Einspieler, R.; Hausegger, K. (Department of Radiology, Graz (Austria))

    1990-09-01

    Dynamic I-123 Hippuran renal studies to measure furosemide response (FR) were performed in three groups of patients: (1) 57 patients with renovascular hypertension due to a poststenotic, ischemic kidney; (2) 23 patients with essential hypertension; and (3) 50 nonhypertensive patients with healthy kidneys (control group). FR was observed as renal parenchymal tracer washout within 10 minutes after the injection of 40 mg of furosemide. The retention index (RI) took into consideration the renal parenchymal tracer content before and 10 minutes after furosemide injection. In the control group, the FR was greater than 50% and the RI was less than 20. Patients with essential hypertension revealed no differences in the amounts of FR and RI compared with the control group. In renovascular hypertension, the FR was diminished and the RI was raised significantly. The values of FR and RI showed a good correlation to the degree of the renal artery stenosis before and after percutaneous transluminal angioplasty. It is concluded that the stimulation of diuresis with furosemide and its quantification represent an important additional step in the evaluation of dynamic I-123 Hippuran studies to detect renal ischemia.

  4. /sup 123/I-amphetamine-SPECT in the diagnosis of neurological disorders

    Energy Technology Data Exchange (ETDEWEB)

    Biersack, H.J.; Kreiten, K.; Hartmann, A.; Friedrich, G.; Linck, H.A.; Winkler, C.

    1985-03-01

    In contrast to conventional brain scintigraphy with sup(99m)Tc-pertechnetate, SPECT with /sup 123/I-IMP enables visualization of the brain tissue itself. The relevance of this imaging technique was evaluated in 54 patients with cerebral disorders. SPECT of the brain was performed with a rotating gamma camera. In 6 of 24 epileptic patients, SPECT revealed foci consistent with EEG-findings which were, however, not detected by CCT. In 4 of 25 patients with cerebrovascular disease, hypoperfused areas were detected by SPECT despite negative results obtained with CCT. In 50% (10/20) of the patients with cerebrovascular disease, SPECT showed a greater functional extent of the lesions than CCT. In 3 patients with migraine and normal CCT, regional perfusion disturbancers were found. SPECT with /sup 123/I-labeled amphetamines, therefore, enables diagnosis of functional perfusion disorders and metabolic disturbances that are not revealed by CCT. In addition, SPECT can be used to exactly demonstrate the functional extent of lesions detected by CCT.

  5. Crossed cerebellar diaschisis demonstrated by SPECT in hemiplegic children

    Energy Technology Data Exchange (ETDEWEB)

    Hamano, Shin-ichiro; Nara, Takahiro; Nozaki, Hidetsugu; Fukushima, Kiyomi (Saitama Children' s Medical Center, Iwatsuki (Japan)); Imai, Masayuki; Kumagai, Koumei; Maekawa, Kihei

    1991-01-01

    Crossed cerebellar diaschisis (CCD) in twenty five children with hemiplegia were studied using single photon emission computed tomography (SPECT) with N-isopropyl-p-I-123-iodoamphetamine. Seven of twenty-five patients had cerebral palsy, and the others were impaired by acquired brain injury between ten months and fourteen years of age. CCD was demonstrated in five patients (20%), who were impaired by acquired brain injury after seven years of age. CCD could never be detected in patients with cerebral palsy. Ipsilateral cerebellar diaschisis was also demonstrated in two patients with cerebral palsy and three with early acquired brain injury before three years of age. It is suggested that diaschisis presents itself as a different form in a contralateral and ipsilateral cerebellum before three years of age from a form which presents after seven years of age. (author).

  6. RNA-binding IMPs promote cell adhesion and invadopodia formation

    DEFF Research Database (Denmark)

    Vikesaa, Jonas; Hansen, Thomas V O; Jønson, Lars

    2006-01-01

    Oncofetal RNA-binding IMPs have been implicated in mRNA localization, nuclear export, turnover and translational control. To depict the cellular actions of IMPs, we performed a loss-of-function analysis, which showed that IMPs are necessary for proper cell adhesion, cytoplasmic spreading...... and invadopodia formation. Loss of IMPs was associated with a coordinate downregulation of mRNAs encoding extracellular matrix and adhesion proteins. The transcripts were present in IMP RNP granules, implying that IMPs were directly involved in the post-transcriptional control of the transcripts. In particular......, we show that a 5.0 kb CD44 mRNA contained multiple IMP-binding sites in its 3'UTR, and following IMP depletion this species became unstable. Direct knockdown of the CD44 transcript mimicked the effect of IMPs on invadopodia, and we infer that CD44 mRNA stabilization may be involved in IMP...

  7. IMP-8. Volume 2: Scientific section. [Bibliography

    Science.gov (United States)

    1980-01-01

    Results of the analysis of the IMP-8 data, which was collected during the first six and one-half years after launch of the IMP-8 spacecraft are presented. The plasma wave experiment data were processed and are available in an easily accessible summary form. These data continue to provide a valuable source for comparative studies with plasma wave experiments on other spacecraft operating in the solar wind and within the Earth's magnetosphere.

  8. IMp: The customizable LEGO® Pinned Insect Manipulator

    Directory of Open Access Journals (Sweden)

    Steen Dupont

    2015-02-01

    Full Text Available We present a pinned insect manipulator (IMp constructed of LEGO® building bricks with two axes of movement and two axes of rotation. In addition we present three variants of the IMp to emphasise the modular design, which facilitates resizing to meet the full range of pinned insect specimens, is fully customizable, collapsible, affordable and does not require specialist tools or knowledge to assemble.

  9. SPECT in epilepsies; SPECT bei Epilepsien

    Energy Technology Data Exchange (ETDEWEB)

    Stefan, H. [Neurologische Klinik, Univ. Erlangen-Nuernberg, Zentrum Epilepsie Erlangen (Germany)

    1997-06-01

    Registration of regional cerebral blood flow provides important new data for the investigation of pathophysiological processes in epilepsies. Further to the registration of perfusions, receptor studies are employed for the differentiation of localisation in focal epilepsies. For ultimative issues interictal registrations are supplemented by ictal recordings during the epileptic seizure. The combination of SPECT and electrophysiological registration can contribute to analysis of propagation of focal epileptic activity. (orig.) [Deutsch] Die Messung der regionalen Hirndurchblutung liefert wichtige neue Erkenntnis zur Untersuchung pathophysiologischer Ablaeufe bei Epilepsien. Ergaenzend zur Registrierung der Perfusion koennen Rezeptor-Studien zur Differenzierung der Lokalisation fokaler Epilepsien beitragen. Interiktale Registrierungen werden fuer letztere Fragestellung durch iktuale Messungen waehrend des epileptischen Anfalls ergaenzt. Die Kombination von SPECT und elektrophysiologischen Registrierungen kann zur Analyse der Propagation fokaler epileptischer Aktivitaet beitragen. (orig.)

  10. Quantitative Immuno-SPECT Monitoring of Pretargeted Radioimmunotherapy with a Bispecific Antibody in an Intraperitoneal Nude Mouse Model of Human Colon Cancer

    NARCIS (Netherlands)

    Schoffelen, R.; Graaf, W.T. van der; Sharkey, R.M.; Franssen, G.M.; McBride, W.J.; Chang, C.H.; Bos, D.L.; Goldenberg, D.M.; Oyen, W.J.G.; Boerman, O.C.

    2012-01-01

    The prospects for using pretargeted immuno-SPECT to monitor the response to pretargeted radioimmunotherapy were examined. In this study, a bispecific anticarcinoembryonic antigen (CEACAM5; CD66e) x antihapten monoclonal antibody, TF2, was used in combination with a small (1.5 kD) peptide, IMP288, la

  11. MRI and SPECT findings as a predictive factor for postencephalitic epilepsy

    Energy Technology Data Exchange (ETDEWEB)

    Mizobuchi, Masahiro; Tanaka, Chiharu; Sako, Kazuya; Murakami, Nobuto; Nihira, Atsuko [Nakamura Memorial Hospital, Sapporo (Japan)

    2000-04-01

    Relationship between postencephalitic epilepsy and acute phase symptoms, CSF, EEG and MRI findings was assessed by Kaplan-Meier method. Thirteen of 34 patients manifestated epilepsy including four intractable cases. Women (7/11), herpes simplex virus (6/7), seizures in acute phase (12/18), abnormal findings or MRI (6/7) were statistically significant. Four of nine patients with hyperperfusion area by HMPAO-SPECT in acute phase and two of three patients with hypoperfusion area by IMP-SPECT in recovery phase had epilepsy. Hyperperfusion in acute phase reflected acute seizures and inflammations, whereas hypoperfusion in the recovery phase reflected hypometabolism caused by brain damage. These findings suggest epileptogenicity, MRI and SPECT findings may predict prognosis of postencephalitic epilepsy. (author)

  12. Single photon emission computed tomography with [sup 123]I-IMP in three cases of the neuroleptic malignant syndrome

    Energy Technology Data Exchange (ETDEWEB)

    Nisijima, K. (Dept. of Psychiatry, Jichi Medical School, Tochigi-Ken (Japan)); Matoba, M. (Dept. of Psychiatry, Jichi Medical School, Tochigi-Ken (Japan)); Ishiguro, T. (Dept. of Psychiatry, Jichi Medical School, Tochigi-Ken (Japan))

    1994-05-01

    Single photon emission computed tomography (SPECT) perfusion brain scans using [sup 123]I-N-isopropyl-p-iodoamphetamine ([sup 123]I-IMP) were performed in three patients with the neuroleptic malignant syndrome (NMS). In two accumulation was increased in the left basal ganglia and decreased in the right on the early images during the active phase of NMS; this asymmetry was not seen after recovery. In the third patient two examinations were performed during the active phase; on the first, increased accumulation of [sup 123]I-IMP in the left basal ganglia was found on the early images, but on the second, increased accumulation of tracer was found in the right basal ganglia on the delayed images. These abnormalities disappeared after improvement of the NMS. These results suggest that a disturbance in the basal ganglia is related to the development of NMS. (orig.)

  13. Applications of cerebral SPECT

    Energy Technology Data Exchange (ETDEWEB)

    McArthur, C., E-mail: claire.mcarthur@nhs.net [Department of Neuroradiology, Institute of Neurological Sciences, Glasgow (United Kingdom); Jampana, R.; Patterson, J.; Hadley, D. [Department of Neuroradiology, Institute of Neurological Sciences, Glasgow (United Kingdom)

    2011-07-15

    Single-photon emission computed tomography (SPECT) can provide three-dimensional functional images of the brain following the injection of one of a series of radiopharmaceuticals that crosses the blood-brain barrier and distributes according to cerebral perfusion, neurotransmitter, or cell density. Applications include differentiating between the dementias, evaluating cerebrovascular disease, preoperative localization of epileptogenic foci, diagnosing movement disorders, and evaluation of intracerebral tumours, while also proving a useful research tool. Unlike positronemission tomography (PET), SPECT imaging is widely available and can be performed in any department that has access to a rotating gamma camera. The purpose of this review is to demonstrate the utility of cerebral SPECT and increase awareness of its role in the investigation of neurological and psychiatric disorders.

  14. Molecular composition of IMP1 ribonucleoprotein granules

    DEFF Research Database (Denmark)

    Jønson, Lars; Vikesaa, Jonas; Krogh, Anders

    2007-01-01

    Localized mRNAs are transported to sites of local protein synthesis in large ribonucleoprotein (RNP) granules, but their molecular composition is incompletely understood. Insulin-like growth factor II mRNA-binding protein (IMP) zip code-binding proteins participate in mRNA localization, and in mo......Localized mRNAs are transported to sites of local protein synthesis in large ribonucleoprotein (RNP) granules, but their molecular composition is incompletely understood. Insulin-like growth factor II mRNA-binding protein (IMP) zip code-binding proteins participate in mRNA localization...

  15. Evaluation of cerebral perfusion imaging with N-isopropyl-p-[{sup 123}I]iodoamphetamine (IMP) in the cases of antiphospholipid syndrome

    Energy Technology Data Exchange (ETDEWEB)

    Kato, Toru; Nanbu, Ichiro; Tohyama, Junko; Ohba, Satoru [Nagoya City Univ. (Japan). Faculty of Medicine

    1995-01-01

    Five cases of antiphospholipid syndrome with mild headache, but without any neurological deficits and abnormal findings by CT and MRI, were examined by cerebral blood perfusion SPECT using N-isopropyl-p-[{sup 123}I] iodoamphetamine (IMP). Although three cases were performed quantification of cerebral blood flow with a microsphere method simultaneously, their values were within normal limits. Two of them showed focal low perfusion areas. One case had relatively low perfusion areas in the bilateral occipital lobes and the right temporal lobe, which improved after treatment. One of two had low perfusion in the bilateral occipital lobes. Other three cases only showed ununiformity of radioisotope uptake on the cerebral blood perfusion SPECT. Low perfusion areas in antiphospholipid syndrome might be caused by microarterial thrombosis, microvenous thrombosis or spasms, although they could be reversible. As early irreversible progress of cerebral blood flow, cerebral blood flow SPECT should be performed in cases of antiphospholipid syndrome with neurological complainments. (author).

  16. L-3-[I-123]iodo-alpha-methyltyrosine scintigraphy in carcinoid tumors : Correlation with biochemical activity and comparison with [In-111-DTPA-D-Phe(1)]-octreotide imaging

    NARCIS (Netherlands)

    Jager, PL; Meijer, WG; Kema, IP; Willemse, PHB; Piers, DA; de Vries, EGE

    2000-01-01

    Carcinoid tumors can produce serotonin (5-hydroxytryptamine) and catecholamines from the precursors tryptophan and tyrosine. Our aim was to evaluate the tyrosine analog L-3-[I-123]iodo-alpha -methyltyrosine (IMT) in the detection and the determination of biochemical activity of these tumors in compa

  17. Penser la guerre impérialiste

    Institute of Scientific and Technical Information of China (English)

    LiQiqing

    2005-01-01

    Depuis la guerre froide et surtout depuis le “11 septembre”, notre planète est tout le temps obsédée par le cauchemar des guerres impérialistes et du terrorisme. A la télévision on voit presque chaque jour des informations sur les attentats, les attentats-suicide,

  18. The IMP-H ion composition experiment

    Science.gov (United States)

    Acuna, M. H.; Ogilvie, K. W.

    1971-01-01

    A compact mass energy spectrometer to be flown aboard the IMP-H spacecraft is described. Scientific aims include determination of the relative and absolute fluxes of He-4(2+), He-4 (+), 0(6+), and 0(7+), and He-3(2+).

  19. Multipinhole collimator with 20 apertures for a brain SPECT application

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Tzu-Cheng; Ellin, Justin R.; Shrestha, Uttam; Seo, Youngho, E-mail: youngho.seo@ucsf.edu [Physics Research Laboratory, Department of Radiology and Biomedical Imaging, University of California, San Francisco, California 94107 (United States); Huang, Qiu [School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai 200030 (China); Gullberg, Grant T. [Department of Radiotracer Development and Imaging Technology, Life Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, California 94702 (United States)

    2014-11-01

    Purpose: Several new technologies for single photon emission computed tomography (SPECT) instrumentation with parallel-hole collimation have been proposed to improve detector sensitivity and signal collection efficiency. Benefits from improved signal efficiency include shorter acquisition times and lower dose requirements. In this paper, the authors show a possibility of over an order of magnitude enhancement in photon detection efficiency (from 7.6 × 10{sup −5} to 1.6 × 10{sup −3}) for dopamine transporter (DaT) imaging of the striatum over the conventional SPECT parallel-hole collimators by use of custom-designed 20 multipinhole (20-MPH) collimators with apertures of 0.75 cm diameter. Methods: Quantifying specific binding ratio (SBR) of {sup 123}I-ioflupane or {sup 123}I-iometopane’s signal at the striatal region is a common brain imaging method to confirm the diagnosis of the Parkinson’s disease. The authors performed imaging of a striatal phantom filled with aqueous solution of I-123 and compared camera recovery ratios of SBR acquired between low-energy high-resolution (LEHR) parallel-hole collimators and 20-MPH collimators. Results: With only two-thirds of total acquisition time (20 min against 30 min), a comparable camera recovery ratio of SBR was achieved using 20-MPH collimators in comparison to that from the LEHR collimator study. Conclusions: Their systematic analyses showed that the 20-MPH collimator could be a promising alternative for the DaT SPECT imaging for brain over the traditional LEHR collimator, which could give both shorter scan time and improved diagnostic accuracy.

  20. Repeated administration of D-amphetamine induces loss of [I-123]FP-CIT binding to striatal dopamine transporters in rat brain: a validation study

    NARCIS (Netherlands)

    J. Booij; K. de Bruin; W.B. Gunning

    2006-01-01

    In recent years, several PET and SPECT studies have shown loss of striatal dopamine transporter (DAT) binding in arnphetamine (AMPH) users. However, the use of DAT SPECT tracers to detect AMPH-induced changes in DAT binding has not been validated. We therefore examined if repeated administration of

  1. Characteristics and management of Enterobacteriaceae harboring IMP-4 or IMP-8 carbapenemase in a tertiary hospital.

    Science.gov (United States)

    Pang, Feng; Jia, Xiu-Qin; Song, Zhen-Zhu; Li, Yan-Hua; Wang, Bin; Zhao, Qi-Gang; Wang, Chuan-Xin; Zhang, Yi; Wang, Le-Xin

    2016-03-01

    The emergence of Enterobacteriaceae harboring IMP-4 or IMP-8 carbapenemases is rare. We report an occurrence of Enterobacteriaceae harboring IMP-4 or IMP-8 carbapenemases in a Chinese tertiary care hospital from November 2010 to December 2012. The clinical characteristics of 30 patients were described. The genetic relationship of isolates was determined by pulsed-field gel electrophoresis (PFGE). Carbapenemases were detected by modified Hodge test (MHT) and polymerase chain reactions (PCRs). Amplicons were sequenced and blasted to determine the genotype. Most infected patients were from intensive care unit and had complex and serious underlying illnesses requiring mechanical ventilation. PFGE revealed that Klebsiella pneumoniae showed two major PFGE types. Two Klebsiella oxytoca had an indistinguishable PFGE pattern, while four Enterobacter cloacae were different strains. The sequencing studies showed Enterobacteriaceae harboring IMP-4 or IMP-8 carbapenemase in the 23 infected patients. The majority of patients had infections with the carbapenemase-producing Enterobacteriaceae (CPE) strain, most were successfully treated with a range of antibiotics and discharged. It is important to maintain a high index of suspicion to screen for carbapenemase-producing Enterobacteriaceae strains. Rapid identification of these strains and implementation of stringent procedures are the key to prevent major outbreaks in a hospital setting.

  2. Quantitation of regional cerebral blood flow by single photon emission computed tomography of CBF-tracer combined with whole-brain CBF; A comparison between [sup 123]I-IMP and [sup 99m]Tc-HMPAO in healthy volunteers

    Energy Technology Data Exchange (ETDEWEB)

    Isaka, Yoshinari; Imaizumi, Masatoshi; Ashida, Keiichi; Ohe, Yosuke; Okamoto, Masaya; Abe, Tohru; Tanaka, Seiji (Osaka National Hospital (Japan))

    1994-05-01

    A simple, noninvasive method of measuring cerebral blood flow (CBF) that uses single-photon emission computed tomography (SPECT) of CBF-tracer and whole brain CBF obtained by xenon-133 ([sup 133]Xe) clearance technique was developed. In nine healthy volunteers, SPECT data were normalized to the count density of [sup 123]I-IMP or [sup 99m]Tc-HMPAO uptake in the whole-brain, and then converted to the absolute units of CBF by multiplying average [sup 133]Xe-CBF in the whole brain. The CBF values measured by [sup 99m]Tc-HMPA CBF-SPECT was significantly lower in the high flow regions of cortical gray matter (bilateral frontal lobe; p<0.05 and right occipital lobe; p<0.05), and was significantly higher in the bilateral white matter (p<0.05 or 0.01) and the cerebellum (p<0.005) compared with the flow values measured by [sup 123]I-IMP CBF-SPECT. Whereas, the IMP-CBF values were significantly lower in the bilateral striatum (p<0.02 or 0.05) compared with the HMPAO-CBF values. Good correlations were found between IMP-CBF and the HMPAO-CBF values in the cortical gray matter (r[sub s]=0.761; p<0.001, n=108), the white matter (r[sub s]=0.739; p<0.001, n=18) and the cerebellum (r[sub s]=0.731; p<0.001, n=18). In the striatum (r[sub s]=0.58; p<0.05, n=18) and the thalamus (r[sub s]=0.628; p<0.05, n=18), the correlations between IMP-CBF and HMPAO-CBF values were inferior to those of the other three regions. The results indicated that the contrast between high and low CBF regions in the HMPAO CBF-SPECT was significantly less than that in the IMP CBF-SPECT. However, this assumption is not applicable in all of the cerebral regions. (author).

  3. Spect in epilepsy; SPECT bei Anfallsleiden

    Energy Technology Data Exchange (ETDEWEB)

    Feistel, H. [Nuklearmedizinische Klinik mit Poliklinik und Neurologische Klinik, Friedrich-Alexander-Univ. Erlangen-Nuernberg, Erlangen (Germany); Weis, M. [Nuklearmedizinische Klinik mit Poliklinik und Neurologische Klinik, Friedrich-Alexander-Univ. Erlangen-Nuernberg, Erlangen (Germany); Michalik, K. [Nuklearmedizinische Klinik mit Poliklinik und Neurologische Klinik, Friedrich-Alexander-Univ. Erlangen-Nuernberg, Erlangen (Germany); Schueler, P. [Nuklearmedizinische Klinik mit Poliklinik und Neurologische Klinik, Friedrich-Alexander-Univ. Erlangen-Nuernberg, Erlangen (Germany); Platsch, G. [Nuklearmedizinische Klinik mit Poliklinik und Neurologische Klinik, Friedrich-Alexander-Univ. Erlangen-Nuernberg, Erlangen (Germany); Stefan, H. [Nuklearmedizinische Klinik mit Poliklinik und Neurologische Klinik, Friedrich-Alexander-Univ. Erlangen-Nuernberg, Erlangen (Germany); Wolf, F. [Nuklearmedizinische Klinik mit Poliklinik und Neurologische Klinik, Friedrich-Alexander-Univ. Erlangen-Nuernberg, Erlangen (Germany)

    1997-06-01

    In the Federal Republic of Germany it is assumed that about 80 000 patients suffer from a focal form of epilepsy which can not be sufficiently controlled with medication. As potential candidates for surgery, these patients undergo stepwise monitoring procedure in which the epileptic focus is located by means of increasingly invasive methods. In Erlangen the periictal SPECT is performed, whereby the perfusion tracer is injected after onset (ictal SPECT), immediately after cessation of the seizure (postictal scan) or between the seizures (interictal scan). To administer the tracer strongly in ictal or postictal state a close functional cooperation between the neurology and nuclear medicine department must be arranged. Injection inside the monitoring unit must be attuned to federal antiradiation precaution law. In temporal lobe epilepsy, different injection-times demonstrate a large area of hyperperfusion after ictal onset, which refines in the first two postictal minutes to the generating focus together with a decreased parietal blood flow pattern. Later, the entire temporal lobe epilepsy an early tracer injection within 40 seconds has to be achieved, otherwise an ictal propagation into distant brain areas, possibly contralateral, may occur. Extratemporal epilepsy is often linked to trauma or congenital malformations, and is difficult to categorize. In difficult cases with equivocal results, efforts can be undertaken by means of receptor scintigraphy with, for example, iomazenil, to localize the focus as a cold lesion caused by neuronal loss. (orig.) [Deutsch] In der Bundesrepublik Deutschland leiden nach vorsichtigen Schaetzungen ca. 80 000 Patienten an einer fokalen Epilepsieform, die mit Medikamenten nur unzureichend kontrolliert werden kann. Als potentielle Kandidaten fuer einen epilepsiechirurgischen Eingriff werden sie einem intensiven Monitoringverfahren unterzogen, in dem stufenweise nach dem Grad der Invasivitaet gesteigert, verschiedene Moeglichkeiten zu

  4. 6-[F-18]Fluoro-L-Dihydroxyphenylalanine Positron Emission Tomography Is Superior to Conventional Imaging with I-123-Metaiodobenzylguanidine Scintigraphy, Computer Tomography, and Magnetic Resonance Imaging in Localizing Tumors Causing Catecholamine Excess

    NARCIS (Netherlands)

    Fiebrich, Helle-Brit; Brouwers, Adrienne H.; Kerstens, Michiel N.; Pijl, Milan E. J.; Kema, Ido P.; de Jong, Johan R.; Jager, Pieter L.; Elsinga, Philip H.; Dierckx, Rudi A. J. O.; van der Wal, Jacqueline E.; Sluiter, Wim J.; de Vries, Elisabeth G. E.; Links, Thera P.

    2009-01-01

    Context: Catecholamine excess is rare, but symptoms may be life threatening. Objective: The objective of the study was to investigate the sensitivity of 6-[F-18]fluoro-L-dihydroxyphenylalanine positron emission tomography (F-18-DOPAPET), compared with I-123-metaiodobenzylguanidine (I-123-MIBG) scint

  5. Use of I-123 MIBG cardiac scintigraphy to assess the impact of carvedilol on cardiac adrenergic neuronal function in childhood dilated cardiomyopathy; Interet de la scintigraphie cardiaque a l'I-123 MIBG pour evaluer l'impact du carvedilol sur la fonction neuronale adrenergique cardiaque dans les myocardiopathies dilatees de l'enfant

    Energy Technology Data Exchange (ETDEWEB)

    Maunoury, C. [Hopital Europeen Georges Pompidou (HEGP), Dept. de Physiologie et Radio-Isotopes, 75 - Paris (France); Acar, P. [Centre Hospitalier Universitaire, Service de Cardiologie Pediatrique, Hopital des Enfants, 31 - Toulouse (France); Sidi, D. [Centre Hospitalier Universitaire Necker-Enfants-Malades, 75 - Paris (France)

    2006-04-15

    I-123 MIBG cardiac scintigraphy is a useful tool to assess cardiac adrenergic neuronal function, which is impaired in children with dilated cardiomyopathy (DCM). In adults with DCM, long-term treatment with carvedilol improves both cardiac adrenergic neuronal function and left ventricular function. The aim of this prospective study was to evaluate the impact of carvedilol on cardiac adrenergic neuronal function and on left ventricular function in seventeen patients (11 female, 6 male, mean age 39 {+-} 57 months, range 1 - 168 months) with DCM. All patients underwent I-123 MIBG cardiac scintigraphy and equilibrium radio-nuclide angiography before and after a 6 month period of carvedilol therapy. A static anterior view of the chest was acquired 4 hours after intravenous injection of 20 to 75 MBq of I-123 MIBG. Cardiac neuronal uptake of I-123 MIBG was measured using the heart to mediastinum count ratio (HMR). Radionuclide left ventricular ejection fraction (LVEF) was assessed following a standard protocol. There was no major cardiac events (death or transplantation) during the follow-up period. I-123 MIBG cardiac uptake and left ventricular function respectively increased by 38% and 65% after 6 months of treatment with carvedilol (HMR 223 {+-} 49% vs 162 {+-} 26%, p < 0.0001 and LVEF = 43 {+-} 17% vs 26 {+-} 11%, p < 0.0001). Carvedilol can improve cardiac adrenergic neuronal function and left ventricular function in children with DCM. Further studies are needed to assess the relationship between improvement in I-123 MIBG cardiac uptake and the beneficial effects of carvedilol on morbidity and mortality. (authors)

  6. Benchmark of different CFL conditions for IMPES

    Science.gov (United States)

    Franc, Jacques; Horgue, Pierre; Guibert, Romain; Debenest, Gerald

    2016-10-01

    The IMplicit Pressure Explicit Saturation (IMPES) method is a prevalent way to simulate multiphase flows in porous media. The numerical stability of this sequential method implies limitations on the time step, which may depend on the flow regime studied. In this note, three stability criteria related to the IMPES method, that differ in their construction on the observed variables, are compared on homogeneous and heterogeneous configurations for different two-phase flow regimes (viscous/capillary/gravitational). This highlights that there is no single optimal criterion always ensuring stability and efficiency. For capillary dominated flows, the Todd's condition is the most efficient one, while the standard Coat condition should be preferred for viscous flows. When gravity effects are present, Coat's condition must be restricted, but remains more efficient than the Todd's condition. xml:lang="fr"

  7. Efficacy of I-123/I-131 Metaiodobenzylguanidine Scan as A Single Initial Diagnostic Modality in Pheochromocytoma: Comparison with Biochemical Test and Anatomic Imaging

    Energy Technology Data Exchange (ETDEWEB)

    Moon, Eun Ha; Lim, Seok Tae; Jeong, Young Jin; Kim, Dong Wook; Jeong, Hwan Jeong; Sohn, Myung Hee [Chonbuk National University Medical School and Hospital, Jeonju (Korea, Republic of)

    2009-10-15

    We underwent this study to evaluate the diagnostic potential of I-123/I-131 metaiodobenzylguanidine (MIBG) scintigraphy alone in the initial diagnosis of pheochromocytoma, compared with biochemical test and anatomic imaging. Twenty two patients (M:F=13:9, Age: 44.3{+-} 19.3 years) having the clinical evaluation due to suspicious pheochromocytoma received the biochemical test, anatomic imaging modality (CT and/or MRI) and I-123/I-131 MIBG scan for diagnosis of pheochromocytoma, prior to histopathological confirmation. MIBG scans were independently reviewed by 2 nuclear medicine physicians. All patients were confirmed histopathologically by operation or biopsy (incisional or excisonal). In comparison of final diagnosis and findings of each diagnostic modality, the sensitivities of the biochemical test, anatomic imaging, and MIBG scan were 88.9%, 55.6%, and 88.9%, respectively. And the specificities of the biochemical test, anatomic imaging, and MIBG scan also were 69.2%, 69.2%, and 92.3%, respectively. MIBG scan showed one false positive (neuroblastoma) and one false negative finding. There was one patient with positive MIBG scan and negative findings of the biochemical test, anatomic imaging. Our data suggest that I-123/I-131 MIBG scan has higher sensitivity, specificity, positive predictive value, negative predictive value and accuracy than those of biochemical test and anatomic imaging. Thus, we expect that MIBG scan is electively used for initial diagnosis of pheochromocytoma alone as well as biochemical test and anatomic imaging.

  8. Expression of IGF-II mRNA-binding proteins (IMPs) in gonads and testicular cancer

    DEFF Research Database (Denmark)

    Hammer, Niels A; Hansen, Thomas v O; Byskov, Anne Grete

    2005-01-01

    prompted us to examine their possible involvement in testicular neoplasia. IMPs were detected primarily in germ-cell neoplasms, including preinvasive testicular carcinoma in situ, classical and spermatocytic seminoma, and nonseminomas, with particularly high expression in undifferentiated embryonal...... carcinoma. The relative expression of IMP1, IMP2 and IMP3 varied among tumor types and only IMP1 was detected in all carcinoma in situ cells. Thus IMPs, and in particular IMP1, may be useful auxiliary markers of testicular neoplasia....

  9. IMP 8 magnetosheath field comparisons with models

    Directory of Open Access Journals (Sweden)

    Z. Kaymaz

    Full Text Available This study presents cross-sectional vector maps of the magnetic field derived from IMP 8 magnetometer in the magnetosheath at 30 Re behind the Earth. In addition the vector patterns of the magnetosheath field for northward, southward, and east-west interplanetary magnetic field (IMF directions are qualitatively compared with those obtained from the Spreiter-Stahara gas dynamic (GD and Fedder-Lyon magnetohydrodynamic models (MHD. The main purpose is to display the cross-sectional differences in relation to the dayside merging with different IMF directions, allowing the reader to make direct visual comparisons of the vector patterns. It is seen that for east-west IMF directions, the data-based and MHD-based patterns differ noticeably in a similar way from the GD model, presumably reflecting the influence of dayside magnetic merging of the Earth's magnetic field with the y-component of the interplanetary magnetic field. All three northward IMF cross sections show comparable field draping patterns as expected for a closed magnetosphere. For southward IMF case, on the other hand, differences between the three cross-sectional patterns are greater as seen in the field vector sizes and directions, especially closer to the magnetopause where more disturbed magnetospheric conditions are known to be exist. The data comparisons with the MHD and GD models show that the differences result from the magnetic field-flow coupling and that the effects of dayside reconnection are present in IMP 8 magnetic field measurements.

    Keywords. Vector maps · IMP 8 magnetometer · Inter-planetary magnetic fields

  10. IMp: The customizable LEGO(®) Pinned Insect Manipulator.

    Science.gov (United States)

    Dupont, Steen; Price, Benjamin; Blagoderov, Vladimir

    2015-01-01

    We present a pinned insect manipulator (IMp) constructed of LEGO® building bricks with two axes of movement and two axes of rotation. In addition we present three variants of the IMp to emphasise the modular design, which facilitates resizing to meet the full range of pinned insect specimens, is fully customizable, collapsible, affordable and does not require specialist tools or knowledge to assemble.

  11. Radiopharmaceuticals for SPECT cancer detection

    Science.gov (United States)

    Chernov, V. I.; Medvedeva, A. A.; Zelchan, R. V.; Sinilkin, I. G.; Stasyuk, E. S.; Larionova, L. A.; Slonimskaya, E. M.; Choynzonov, E. L.

    2016-08-01

    The purpose of the study was to assess the efficacy of single photon emission computed tomography (SPECT) with 199Tl and 99mTc-MIBI in the detection of breast, laryngeal and hypopharyngeal cancers. A total of 220 patients were included into the study: 120 patients with breast lesions (100 patients with breast cancer and 20 patients with benign breast tumors) and 100 patients with laryngeal/hypopharyngeal diseases (80 patients with laryngeal/hypopharyngeal cancer and 20 patients with benign laryngeal/hypopharyngeal lesions). No abnormal 199Tl uptake was seen in all patients with benign breast and laryngeal lesions, indicating a 100% specificity of 199Tl SPECT. In the breast cancer patients, the increased 199Tl uptake in the breast was visualized in 94.8% patients, 99mTc-MIBI—in 93.4% patients. The increased 199Tl uptake in axillary lymph nodes was detected in 60% patients, and 99mTc-MIBI—in 93.1% patients. In patients with laryngeal/hypopharyngeal cancer, the sensitivity of SPECT with 199Tl and 99mTc-MIBI was 95%. The 199Tl SPECT sensitivity in identification of regional lymph node metastases in the patients with laryngeal/hypopharyngeal cancer was 75% and the 99mTc-MIBI SPECT sensitivity was 17%. The data obtained showed that SPECT with 199Tl and 99mTc-MIBI can be used as one of the additional imaging methods in detection of tumors.

  12. Molecular imaging agents for SPECT (and SPECT/CT)

    Energy Technology Data Exchange (ETDEWEB)

    Gnanasegaran, Gopinath [Guy' s and St Thomas' NHS Foundation Trust, Department of Nuclear Medicine, London (United Kingdom); Ballinger, James R. [Guy' s and St Thomas' NHS Foundation Trust, Department of Nuclear Medicine, London (United Kingdom); King' s College London, Division of Imaging Sciences and Biomedical Engineering, London (United Kingdom)

    2014-05-15

    The development of hybrid single photon emission computed tomography/computed tomography (SPECT/CT) cameras has increased the diagnostic value of many existing single photon radiopharmaceuticals. Precise anatomical localization of lesions greatly increases diagnostic confidence in bone imaging of the extremities, infection imaging, sentinel lymph node localization, and imaging in other areas. Accurate anatomical localization is particularly important prior to surgery, especially involving the parathyroid glands and sentinel lymph node procedures. SPECT/CT plays a role in characterization of lesions, particularly in bone scintigraphy and radioiodine imaging of metastatic thyroid cancer. In the development of novel tracers, SPECT/CT is particularly important in monitoring response to therapies that do not result in an early change in lesion size. Preclinical SPECT/CT devices, which actually have spatial resolution superior to PET/CT devices, have become essential in characterization of the biodistribution and tissue kinetics of novel tracers, allowing coregistration of serial studies within the same animals, which serves both to reduce biological variability and reduce the number of animals required. In conclusion, SPECT/CT increases the utility of existing radiopharmaceuticals and plays a pivotal role in the evaluation of novel tracers. (orig.)

  13. Molecular imaging of angiogenesis with SPECT.

    NARCIS (Netherlands)

    Dijkgraaf, I.; Boerman, O.C.

    2010-01-01

    Single-photon emission computed tomography (SPECT) and position emission tomography (PET) are the two main imaging modalities in nuclear medicine. SPECT imaging is more widely available than PET imaging and the radionuclides used for SPECT are easier to prepare and usually have a longer half-life th

  14. Progress in BazookaSPECT.

    Science.gov (United States)

    Miller, Brian W; Barber, H Bradford; Furenlid, Lars R; Moore, Stephen K; Barrett, Harrison H

    2009-01-01

    Recent progress on a high-resolution, photon-counting gamma-ray and x-ray imager called BazookaSPECT is presented. BazookaSPECT is an example of a new class of scintillation detectors based on integrating detectors such as CCD(charge-coupled device) or CMOS(complementary metal-oxide semiconductor) sensors. BazookaSPECT is unique in that it makes use of a scintillator in close proximity to a microchannel plate-based image intensifier for up-front optical amplification of scintillation light. We discuss progress made in bringing about compact BazookaSPECT modules and in real-time processing of event data using graphics processing units (GPUs). These advances are being implemented in the design of a high-resolution rodent brain imager called FastSPECT III. A key benefit of up-front optical gain is that any CCD/CMOS sensor can now be utilized for photon counting. We discuss the benefits and feasibility of using CMOS sensors as photon-counting detectors for digital radiography, with application in mammography and computed tomography (CT). We present as an appendix a formal method for comparing various photon-counting integrating detectors using objective statistical criteria.

  15. SPECT in Focal Epilepsies

    Directory of Open Access Journals (Sweden)

    Roderick Duncan

    2000-01-01

    Full Text Available Brain perfusion changes during seizures were first observed in the 1930s. Single Photon Emission Computed Tomography (SPECT was developed in the 1970s, and tracers suitable for the imaging of regional cerebral perfusion (rCP became available in the 1980s. The method was first used to study rCP in the interictal phase, and this showed areas of low perfusion in a proportion of cases, mainly in patients with temporal lobe epilepsies. However, the trapping paradigm of tracers such as hexamethyl propyleneamine oxime (HMPAO provided a practicable method of studying changes in rCP during seizures, and a literature was established in the late 1980s and early 1990s showing a typical sequence of changes during and after seizures of mesial temporal lobe origin; the ictal phase was associated with large increases in perfusion throughout the temporal lobe, with first the lateral, then the mesial temporal lobe becoming hypoperfused in the postictal phase. Activation and inhibition of other structures, such as the basal ganglia and frontal cortex, were also seen. Studies of seizures originating elsewhere in the brain have shown a variety of patterns of change, according to the structures involved. These changes have been used practically to aid the process of localisation of the epileptogenic zone so that epilepsy surgery can be planned.

  16. Quantitative image reconstruction for dual-isotope parathyroid SPECT/CT: phantom experiments and sample patient studies

    Science.gov (United States)

    Shcherbinin, S.; Chamoiseau, S.; Celler, A.

    2012-08-01

    We investigated the quantitative accuracy of the model-based dual-isotope single-photon emission computed tomography (DI-SPECT) reconstructions that use Klein-Nishina expressions to estimate the scattered photon contributions to the projection data. Our objective was to examine the ability of the method to recover the absolute activities pertaining to both radiotracers: Tc-99m and I-123. We validated our method through a series of phantom experiments performed using a clinical hybrid SPECT/CT camera (Infinia Hawkeye, GE Healthcare). Different activity ratios and different attenuating media were used in these experiments to create cross-talk effects of varying severity, which can occur in clinical studies. Accurate model-based corrections for scatter and cross-talk with CT attenuation maps allowed for the recovery of the absolute activities from DI-SPECT/CT scans with errors that ranged 0-10% for both radiotracers. The unfavorable activity ratios increased the computational burden but practically did not affect the resulting accuracy. The visual analysis of parathyroid patient data demonstrated that our model-based processing improved adenoma/background contrast and enhanced localization of small or faint adenomas.

  17. Organ volume estimation using SPECT

    CERN Document Server

    Zaidi, H

    1996-01-01

    Knowledge of in vivo thyroid volume has both diagnostic and therapeutic importance and could lead to a more precise quantification of absolute activity contained in the thyroid gland. In order to improve single-photon emission computed tomography (SPECT) quantitation, attenuation correction was performed according to Chang's algorithm. The dual-window method was used for scatter subtraction. We used a Monte Carlo simulation of the SPECT system to accurately determine the scatter multiplier factor k. Volume estimation using SPECT was performed by summing up the volume elements (voxels) lying within the contour of the object, determined by a fixed threshold and the gray level histogram (GLH) method. Thyroid phantom and patient studies were performed and the influence of 1) fixed thresholding, 2) automatic thresholding, 3) attenuation, 4) scatter, and 5) reconstruction filter were investigated. This study shows that accurate volume estimation of the thyroid gland is feasible when accurate corrections are perform...

  18. PET and SPECT in neurology

    Energy Technology Data Exchange (ETDEWEB)

    Dierckx, Rudi A.J.O. [Groningen University Medical Center (Netherlands). Dept. of Nuclear Medicine and Molecular Imaging; Ghent Univ. (Belgium). Dept. of Radiology and Nuclear Medicine; Vries, Erik F.J. de; Waarde, Aren van [Groningen University Medical Center (Netherlands). Dept. of Nuclear Medicine and Molecular Imaging; Otte, Andreas (ed.) [Univ. of Applied Sciences Offenburg (Germany). Faculty of Electrical Engineering and Information Technology

    2014-07-01

    PET and SPECT in Neurology highlights the combined expertise of renowned authors whose dedication to the investigation of neurological disorders through nuclear medicine technology has achieved international recognition. Classical neurodegenerative disorders are discussed as well as cerebrovascular disorders, brain tumors, epilepsy, head trauma, coma, sleeping disorders, and inflammatory and infectious diseases of the CNS. The latest results in nuclear brain imaging are detailed. Most chapters are written jointly by a clinical neurologist and a nuclear medicine specialist to ensure a multidisciplinary approach. This state-of-the-art compendium will be valuable to anybody in the field of neuroscience, from the neurologist and the radiologist/nuclear medicine specialist to the interested general practitioner and geriatrician. It is the second volume of a trilogy on PET and SPECT imaging in the neurosciences, the other volumes covering PET and SPECT in psychiatry and in neurobiological systems.

  19. Characterization of the Eimeria maxima sporozoite surface protein IMP1.

    Science.gov (United States)

    Jenkins, M C; Fetterer, R; Miska, K; Tuo, W; Kwok, O; Dubey, J P

    2015-07-30

    The purpose of this study was to characterize Eimeria maxima immune-mapped protein 1 (IMP1) that is hypothesized to play a role in eliciting protective immunity against E. maxima infection in chickens. RT-PCR analysis of RNA from unsporulated and sporulating E. maxima oocysts revealed highest transcription levels at 6-12h of sporulation with a considerable downregulation thereafter. Alignment of IMP1 coding sequence from Houghton, Weybridge, and APU-1 strains of E. maxima revealed single nucleotide polymorphisms that in some instances led to amino acid changes in the encoded protein sequence. The E. maxima (APU-1) IMP1 cDNA sequence was cloned and expressed in 2 different polyHis Escherichia coli expression vectors. Regardless of expression vector, recombinant E. maxima IMP1 (rEmaxIMP1) was fairly unstable in non-denaturing buffer, which is consistent with stability analysis of the primary amino acid sequence. Antisera specific for rEmaxIMP1 identified a single 72 kDa protein or a 61 kDa protein by non-reducing or reducing SDS-PAGE/immunoblotting. Immunofluorescence staining with anti-rEmaxIMP1, revealed intense surface staining of E. maxima sporozoites, with negligible staining of merozoite stages. Immuno-histochemical staining of E. maxima-infected chicken intestinal tissue revealed staining of E. maxima developmental stages in the lamnia propia and crypts at both 24 and 48 h post-infection, and negligible staining thereafter. The expression of IMP1 during early stages of in vivo development and its location on the sporozoite surface may explain in part the immunoprotective effect of this protein against E. maxima infection.

  20. Simulation of proton radiography terminal at IMP

    CERN Document Server

    Yan, Yan; Huang, Zhi-Wu; Wang, Jie; Yao, Ze-En; Wang, Jun-Run; Wei, Zheng; Yang, Jian-Cheng; Yuan, You-Jin

    2015-01-01

    Proton radiography is used for advanced hydrotesting as a new type radiography technology due to its powerful penetration capability and high detection efficiency. A new proton radiography terminal will be developed to radiograph static samples at Institute of Modern Physics of Chinese Academy of Science (IMP-CAS). The proton beam with the maximum energy of 2.6 GeV will be produced by Heavy Ion Research Facility in Lanzhou-Cooling Storage Ring (HIRFL-CSR). The proton radiography terminal consists of the matching magnetic lens and the Zumbro lens system. In this paper, the design scheme and all optic parameters of this beam terminal for 2.6GeV proton energy are presented by simulating the beam optics using WINAGILE code. My-BOC code is used to test the particle tracking of proton radiography beam line. Geant4 code and G4beamline code are used for simulating the proton radiography system. The results show that the transmission efficiency of proton without target is 100%, and the effect of secondary particles ca...

  1. Analysis of regional cerebral blood flow and distribution volume in Machado-Joseph disease by iodine-{sup 123}I IMP single photon emission computed tomography

    Energy Technology Data Exchange (ETDEWEB)

    Hayashi, Tsunemi; Nakajima, Takashi; Fukuhara, Nobuyoshi [National Saigata Hospital, Ohagata, Niigata (Japan)

    2001-09-01

    Machado-Joseph disease (MJD) is an autosomal dominant spinocerebellar ataxia. Its clinical features vary greatly in different generations of the same family. Regional cerebral blood flow (rCBF) and distribution volume (V{sub d}) in the pons, cerebellum, and cerebral cortex were measured in 12 patients with MJD by autoradiography (ARG) and the table look-up (TLU) method of iodine-123 IMP ({sup 123}I-IMP) single photon emission computed tomography (SPECT). Representative cases were as follows: A 46-year-old woman first experienced gait ataxia at age 38. Computed tomography (CT) and magnetic resonance imaging (MRI) showed no atrophy in the pons or cerebellum, but rCBF measured by the {sup 123}I-IMP SPECT ARG method detected hypoperfusion in the pons, and cerebellar vermis and hemisphere. A 76-year-old woman first experienced gait ataxia at age 69. CT and MRI findings showed severe atrophy in the pons, and cerebellar vermis and hemisphere. Moreover, rCBF was decreased in the pons, whereas it was not decreased in the cerebellar vermis and hemisphere. In the pons of patients with MJD, rCBF was markedly decreased regardless of disease severity. Because this SPECT finding for the pons looked like a 'dot', we have called it the 'pontine dot sign'. In the MJD group, rCBF was significantly decreased in the pons (Student's t test, p<0.01) and cerebellar vermis (p<0.05). The V{sub d} was also significantly decreased in the pons (p<0.005) in comparison with that for normal subjects. Pearson's correlation analysis yielded a significant relationship between the rCBF in the pons and age at onset (r=0.578, p<0.05). There was a strong correlation between the V{sub d} for the pons and age at onset (r=0.59, p<0.05). Person's correlation analysis also showed a significant relationship between the V{sub d} in the cerebellar hemispheres and International Cooperative Ataxia Rating Scale (r=0.644, p<0.05). The pontine rCBFs in patients with early onset MJD

  2. PET and SPECT in psychiatry

    Energy Technology Data Exchange (ETDEWEB)

    Dierckx, Rudi A.J.O. [University Medical Center Groningen (Netherlands). Dept. of Nuclear Medicine and Molecular Imaging; Ghent Univ. (Belgium); Otte, Andreas [Univ. of Applied Sciences Offenburg (Germany). Faculty of Electrical Engineering and Information Technology; Vries, Erik F.J. de; Waarde, Aren van (eds.) [University Medical Center Groningen (Netherlands). Dept. of Nuclear Medicine and Molecular Imaging

    2014-09-01

    Covers classical psychiatric disorders as well as other subjects such as suicide, sleep, eating disorders, and autism. Emphasis on a multidisciplinary approach. Written by internationally acclaimed experts. PET and SPECT in Psychiatry showcases the combined expertise of renowned authors whose dedication to the investigation of psychiatric disease through nuclear medicine technology has achieved international recognition. The classical psychiatric disorders as well as other subjects - such as suicide, sleep, eating disorders, and autism - are discussed and the latest results in functional neuroimaging are detailed. Most chapters are written jointly by a clinical psychiatrist and a nuclear medicine expert to ensure a multidisciplinary approach. This state of the art compendium will be valuable to all who have an interest in the field of neuroscience, from the psychiatrist and the radiologist/nuclear medicine specialist to the interested general practitioner and cognitive psychologist. It is the first volume of a trilogy on PET and SPECT imaging in the neurosciences; other volumes will focus on PET and SPECT in neurology and PET and SPECT of neurobiological systems.

  3. SPECT og PET i neurobiologien

    DEFF Research Database (Denmark)

    Paulson, O.B.; Lassen, N.A.

    1997-01-01

    PET (positron emission tomography) and SPECT (single photon emission computed tomography) are isotopic methods in which the distribution is registered of radiolabelled tracers given in such small amounts that they are without effect on the organism or the organism's disposal of them. Thus, a series...

  4. Targeted multi-pinhole SPECT

    NARCIS (Netherlands)

    Branderhorst, W.; Vastenhouw, B.; Van der Have, F.; Blezer, E.L.A.; Bleeker, W.K.; Beekman, F.J.

    2010-01-01

    Purpose: Small-animal single photon emission computed tomography (SPECT) with focused multi-pinhole collimation geometries allows scanning modes in which large amounts of photons can be collected from specific volumes of interest. Here we present new tools that improve targeted imaging of specific

  5. SPECT og PET i neurobiologien

    DEFF Research Database (Denmark)

    Paulson, O B; Lassen, N A

    1997-01-01

    PET (positron emission tomography) and SPECT (single photon emission computed tomography) are isotopic methods in which the distribution is registered of radiolabelled tracers given in such small amounts that they are without effect on the organism or the organism's disposal of them. Thus, a series...

  6. Targeted multi-pinhole SPECT

    Energy Technology Data Exchange (ETDEWEB)

    Branderhorst, Woutjan; Blezer, Erwin L.A. [University Medical Centre Utrecht, Image Sciences Institute and Rudolf Magnus Institute of Neuroscience, Utrecht (Netherlands); Vastenhouw, Brendan; Have, Frans van der; Beekman, Freek J. [University Medical Centre Utrecht, Image Sciences Institute and Rudolf Magnus Institute of Neuroscience, Utrecht (Netherlands); Molecular Imaging Laboratories BV, Utrecht (Netherlands); Delft University of Technology, Section of Radiation Detection and Medical Imaging, Applied Sciences, Delft (Netherlands); Bleeker, Wim K. [Genmab BV, Utrecht (Netherlands)

    2011-03-15

    Small-animal single photon emission computed tomography (SPECT) with focused multi-pinhole collimation geometries allows scanning modes in which large amounts of photons can be collected from specific volumes of interest. Here we present new tools that improve targeted imaging of specific organs and tumours, and validate the effects of improved targeting of the pinhole focus. A SPECT system with 75 pinholes and stationary detectors was used (U-SPECT-II). An XYZ stage automatically translates the animal bed with a specific sequence in order to scan a selected volume of interest. Prior to stepping the animal through the collimator, integrated webcams acquire images of the animal. Using sliders, the user designates the desired volume to be scanned (e.g. a xenograft or specific organ) on these optical images. Optionally projections of an atlas are overlaid semiautomatically to locate specific organs. In order to assess the effects of more targeted imaging, scans of a resolution phantom and a mouse myocardial phantom, as well as in vivo mouse cardiac and tumour scans, were acquired with increased levels of targeting. Differences were evaluated in terms of count yield, hot rod visibility and contrast-to-noise ratio. By restricting focused SPECT scans to a 1.13-ml resolution phantom, count yield was increased by a factor 3.6, and visibility of small structures was significantly enhanced. At equal noise levels, the small-lesion contrast measured in the myocardial phantom was increased by 42%. Noise in in vivo images of a tumour and the mouse heart was significantly reduced. Targeted pinhole SPECT improves images and can be used to shorten scan times. Scan planning with optical cameras provides an effective tool to exploit this principle without the necessity for additional X-ray CT imaging. (orig.)

  7. SPECT analysis of recent cerebral infarction

    DEFF Research Database (Denmark)

    Raynaud, C; Rancurel, G; Tzourio, N

    1989-01-01

    already differentiated in the subacute period. The central area presented a short phase of luxury perfusion and a longer phase of IMP hyperfixation. The peripheral area showed both a slight regional cerebral blood flow decrease and an early IMP uptake decrease similar to those previously found...

  8. Single photon emission computed tomography with [sup 123]I-N-isopropyl-p-iodoamphetamine in three cases of neuroleptic malignant syndrome

    Energy Technology Data Exchange (ETDEWEB)

    Nishijima, Koichi; Matoba, Masaki; Takano, Kenji; Ishiguro, Takeo (Jichi Medical School, Minamikawachi, Tochigi (Japan)); Nakamura, Megumi; Nagano, Mitsuru

    1992-11-01

    Three patients with neuroleptic malignant syndrome underwent single photon emission computd tomography (SPECT) with I-123-N-isopropyl-p-iodoamphetamine (I-123 IMP). In two patients, there was bilateral difference in tracer uptake on early images: decreased uptake in the right basal ganglion and increased uptake in the left basal ganglion. In the third patient, similar findings were seen on early images; however, in contrast to early images, delayed images showed more noticeable tracer uptake in the right basal ganglion than the left basal ganglion. These findings were not associated with the recovery from neuroleptic malignant syndrome. This suggested that some abnormality in the basal ganglion may be involved in the occurrence of neuroleptic malignant syndrome. SPECT with I-123 IMP was considered useful in searching for pathophysiology of neuroleptic malignant syndrome. (N.K.).

  9. A new method of regional cerebral blood flow measurement using one-point arterial sampling based on the microsphere model with N-isopropyl-p-[[sup 123]I]-iodoamphetamine SPECT

    Energy Technology Data Exchange (ETDEWEB)

    Odano, I.; Takahashi, N.; Higuchi, T. (Niigata Univ. (Japan). School of Medicine); Ohkubo, M. (Niigata Univ. (Japan). Coll. of Biomedical Technology)

    1994-07-01

    We developed a new method for quantitative measurement of regional cerebral blood flow (rCBF) using one-point arterial sampling with N-isopropyl-p-[[sup 123]I]-iodoamphetamine ([sup 123]I-IMP) and single photon emission computed tomography (SPECT) based on the microsphere model. The one-point Ca(t) method provides fast, easy, accurate and non-invasive measurement of rCBF without inserting catheters and without treatment of arterial blood with octanol. (author).

  10. Improvement of tomographic reconstruction in bone SPECT

    Energy Technology Data Exchange (ETDEWEB)

    Schuenemann, M.; Sahlmann, C.O.; Siefker, U.; Luig, H.; Meller, J. [Abteilungen fuer Nuklearmedizin, Georg-August-Univ. Goettingen (Germany); Heidrich, G. [Abteilungen fuer Diagnostische Radiologie, Georg-August-Univ., Goettingen (Germany); Werner, C.; Brunner, E. [Abteilungen fuer Medizinische Statistik, Georg-August-Univ., Goettingen (Germany)

    2006-07-01

    Aim: the comparison between iterative reconstruction and filtered backprojection in the reconstruction of bone SPECT in the diagnosis of skeletal metastases. Patients, methods: 47 consecutive patients (vertebral segments: n = 435), with suspected malignancy of the vertebral column, were examined by bone scintigraphy and MRI (maximal interval between the two procedures {+-} 5 weeks). The SPECT-data were reconstructed with an iterative algorithm (ISA) and with filtered backprojection. We defined semiquantitative criteria in order to assess the quality of the tomograms. Conventional reconstruction was performed both by a Wiener-filter and a low-pass-filter. Iterative reconstruction was performed by the ISA algorithm. The clinical evaluation of the different reconstruction algorithms was performed by MRI as the gold-standard. Results: sensitivity (%): 87.3 (ISA), 86.4 (low-pass), 79.7 (Wiener); specificity (%): 95.3 (ISA), 95 (low-pass), 85.4 (Wiener). The sensitivity of iterative reconstructed SPECT and low-pass reconstructed SPECT was significantly higher (p < 0.05) compared with the sensitivity of SPECT reconstructed by the Wiener-filter. The specificity of iterative reconstruction ISA and low-pass-filter reconstructed SPECT were significantly higher compared with the SPECT data reconstructed by the Wiener-filter. ISA was significantly superior to the Wiener-SPECT relating to all criteria of quality. Iterative reconstruction was significantly superior to the low-pass-SPECT relating to 2 of 3 criteria. In addition the Wiener-SPECT was significantly inferior to the low-pass-SPECT regarding to 2 of 3 criteria. Conclusion: in our series the iterative algorithm ISA was the method of choice in the reconstruction of bone SPECT data. In comparison with conventional algorithms ISA offers a significantly higher quality of the tomograms and yields a high diagnostic accuracy. (orig.)

  11. IMP: A Message-Passing Algorithmfor Matrix Completion

    CERN Document Server

    Kim, Byung-Hak; Pfister, Henry D

    2010-01-01

    A new message-passing (MP) method is considered for the matrix completion problem associated with recommender systems. We attack the problem using a (generative) factor graph model that is related to a probabilistic low-rank matrix factorization. Based on the model, we propose a new algorithm, termed IMP, for the recovery of a data matrix from incomplete observations. The algorithm is based on a clustering followed by inference via MP (IMP). The algorithm is compared with a number of other matrix completion algorithms on real collaborative filtering (e.g., Netflix) data matrices. Our results show that, while many methods perform similarly with a large number of revealed entries, the IMP algorithm outperforms all others when the fraction of observed entries is small. This is helpful because it reduces the well-known cold-start problem associated with collaborative filtering (CF) systems in practice.

  12. First Report of bla(IMP-8) in Raoultella planticola.

    Science.gov (United States)

    Tseng, Sung-Pin; Wang, Jann-Tay; Liang, Chih-Yuan; Lee, Pei-Shan; Chen, Yee-Chun; Lu, Po-Liang

    2014-01-01

    Two carbapenem-resistant Raoultella planticola clinical isolates were isolated from patients with pneumonia and Port-A catheter-related bacteremia, respectively, in Taiwan. These isolates remained susceptible to fluoroquinolone, aminoglycoside, and colistin. Though the two isolates had the same antibiogram, plasmidic carbapenemase blaIMP-8, class 1 integron cassette (dfrA12-orfF-aadA2), and qnrB2, they had different pulsed-field gel electrophoresis patterns, plasmid sizes, and outer membrane protein loss profiles. To our knowledge, this is the first report of blaIMP-8 found in R. planticola. Interestingly, blaIMP-8 is the most common carbapenemase found in Klebsiella pneumoniae in Taiwan. In the literature, carbapenemase genes in R. planticola in each country were also found in carbapenem-resistant Enterobacteriaceae in the same country.

  13. SPECT of aged backache patients

    Energy Technology Data Exchange (ETDEWEB)

    Ito, Shigehiko; Nishikimi, Junzo; Mizuno, Naokado; Watanabe, Kentaro; Kondo, Masaki; Ozaki, Satoshi; Urasaki, Tetsuya; Muro, Toshiyuki [Prefectural Tajimi Hospital, Gifu (Japan)

    1995-12-01

    Single photon emission computed tomography (SPECT) using {sup 99m}Tc-HMDP was performed on 53 middle-aged or elderly patients (male 20, female, 33; age range, 40-80 years old) with lumbago, i.e., 25 patients with lumbar spondylosis, 15 with lumbar degenerative spondylolisthesis, 4 with spondylolytic spondylolisthesis, 3 with compression fracture, 3 with pulurent spondylitis, 2 with spondylous osteoporosis, and 1 with spinal osteodesmosis. {sup 99m}Tc-HMDP (740 MBq) was intravenously injected and regular SPECT was performed at 3 hours. Gamma camera was performed for about 10 seconds with 5deg intervals, and 36 steps (180deg) of collection was completed after about 6 minutes. The radioisotope accumulation, the presence or absence of sthenia, and its site were evaluated. Forty-seven (88.7%) patients showed excessive accumulation, i.e., 40 (75.5%) in peripheral vertebral osteophyte, 31 (58.5%) in vertebral articulations, and 10 (18.9%) in whole vertebral body. Significantly increased bilateral excessive accumulation was admitted in the vertebral articulations of sliding disc in degenerative spondylolisthesis. SPECT is considered useful in understanding the pathophysiology of degenerative lumber diseases. (S.Y.).

  14. The current status of SPECT or SPECT/CT in South Korea

    Energy Technology Data Exchange (ETDEWEB)

    Yoo, Ik Dong; Choi, Eun Kyung; Chung, Yong An [Dept. of Radiology, Incheon Saint Mary' s HospitalThe Catholic University of Korea, Incheon (Korea, Republic of)

    2017-06-15

    The first step to nuclear medicine in Korea started with introduction of the gamma camera in 1969. Although planar images with the gamma camera give important functional information, they have the limitations that result from 2-dimensional images. Single-photon emission computed tomography (SPECT) due to its 3-dimensional image acquisition is superior to earlier planar gamma imaging in image resolution and diagnostic accuracy. As demand for a hybrid functional and anatomical imaging device has increased, integrated SPECT/CT systems have been used. In Korea, SPECT/CT was for the first time installed in 2003. SPECT/CT can eliminate many possible pitfalls on SPECT-alone images, making better attenuation correction and thereby improving image quality. Therefore, SPECT/CT is clinically preferred in many hospitals in various aspects. More recently, additional SPECT/CT images taken from the region with equivocal uptake on planar images have been helpful in making precise interpretation as part of their clinical workup in postoperative thyroid cancer patients. SPECT and SPECT/CT have various advantages, but its clinical application has gradually decreased in recent few years. While some researchers investigated the myocardial blood flow with cardiac PET using F-18 FDG or N-13 ammonia, myocardial perfusion SPECT is, at present, the radionuclide imaging study of choice for the risk stratification and guiding therapy in the coronary artery disease patients in Korea. New diagnostic radiopharmaceuticals for AD have received increasing attention; nevertheless, brain SPECT will remain the most reliable modality evaluating cerebral perfusion.

  15. SPECT/CT in neuroendocrine cancers; SPECT/CT bei neuroendokrinen Tumoren

    Energy Technology Data Exchange (ETDEWEB)

    Miederer, M. [Technische Univ. Muenchen (Germany). Nuklearmedizinische Klinik und Poliklinik; Mainz Univ. (Germany). Klinik und Poliklinik fuer Nuklearmedizin; Castrop, C.; Scheidhauer, K. [Technische Univ. Muenchen (Germany). Nuklearmedizinische Klinik und Poliklinik; Buck, A.K. [Universitaetsklinikum Wuerzburg (Germany). Nuklearmedizinische Klinik und Poliklinik

    2011-03-15

    The accuracy of functional SPECT imaging has been significantly improved by addition of CT. This is especially relevant for functional imaging of neuroendocrine tumors using highly specific radiopharmaceuticals. Parathyroid adenomas can be detected by {sup 99m}Tc-MIBI SPECT/CT with very high sensitivity and specificity, playing an important role especially when minimally invasive techniques are used for surgical resection. With SPECT/CT, extra-adrenal manifestations of pheochromocytomas and tumors of the adrenal cortex can be detected with high accuracy. Because of the availability of PET radiopharmaceuticals such as {sup 68}Ga-DOTATOC, the clinical relevance of {sup 111}In-Octreotide SPECT for detection of neuroendocrine cancers has been recently reduced. Because of the better availability, SPECT and SPECT/CT still represent standard tools for imaging neuroendocrine cancers. SPECT/CT represents the superior imaging modality for monitoring radiopeptide based therapies, which are now increasingly used for treatment of neuroendocrine cancers. (orig.)

  16. Emergence of Citrobacter freundii carrying IMP-8 metallo-β-lactamase in Germany

    Science.gov (United States)

    Peter, S; Wolz, C; Kaase, M; Marschal, M; Schulte, B; Vogel, W; Autenrieth, I; Willmann, M

    2014-01-01

    Metallo-β-lactamases (MBLs) in Enterobacteriaceae are an increasing problem worldwide. This report describes the isolation of Citrobacter freundii carrying IMP-8 MBL from three patients during the period from March 2012 until March 2013 in Germany. The blaIMP-8 enzyme is predominantly found in Asia, where IMP-8 has spread to various enterobacterial species causing serious infections. To our best knowledge, this is the first report of blaIMP-8 habouring Enterobacteriaceae in Europe. PMID:25356340

  17. Emergence of Citrobacter freundii carrying IMP-8 metallo-β-lactamase in Germany

    Directory of Open Access Journals (Sweden)

    S. Peter

    2014-03-01

    Full Text Available Metallo-β-lactamases (MBLs in Enterobacteriaceae are an increasing problem worldwide. This report describes the isolation of Citrobacter freundii carrying IMP-8 MBL from three patients during the period from March 2012 until March 2013 in Germany. The blaIMP-8 enzyme is predominantly found in Asia, where IMP-8 has spread to various enterobacterial species causing serious infections. To our best knowledge, this is the first report of blaIMP-8 habouring Enterobacteriaceae in Europe.

  18. Diagnostic value of SPECT in bone scintigraphy; Bedeutung der SPECT bei der Knochenszintigraphie

    Energy Technology Data Exchange (ETDEWEB)

    Gratz, S.; Becker, W. [Goettingen Univ. (Germany). Abt. fuer Nuklearmedizin

    2000-05-01

    Single Photon Emission Computed Tomography (SPECT) reflects the realization of its ability to remove unwanted activity from bone structures with greater contrast. Especially in the spine SPECT improves the diagnostic accuracy compared with planar bone scintigraphy, because smaller structures of single vertebral bodies can be diagnosed with better anatomic resolution due to SPECT with consequently higher sensitivities and specificities. With SPECT, alterations of the lateral part of the vertebral body, pars interacticularis and smaller facet joints can be correctly diagnosed as spondylarthrotic degenerative alterations. In patients with suspected spine malignancies SPECT did not demonstrate to be a reliable instrument for diagnosing malignancy. We recommend to perform SPECT in case of patients with back pain and suspected spine pathologies seen on planar bone scans, since a correct diagnosis of especially benign spondylarthrotic spine lesions is possible with SPECT. With SPECT, a reduction of radiological examinations of 23% should be possible. (orig.) [German] Die Single-Photonenemissionscomputertomographie (SPECT) erlaubt bei der Knochenszintigraphie eine ueberlagerungsfreie Darstellung von Knochenstrukturen mit hoher Kontrastgenauigkeit. Insbesondere bei Wirbelsaeulenerkrankungen ermoeglicht SPECT einen deutlichen diagnostischen Zugewinn gegenueber planaren Aufnahmen, da kleinere Strukturen einzelner Wirbelkoerper aufgrund des hohen anatomischen Aufloesungsvermoegens mit SPECT sehr sensitiv und spezifisch dargestellt werden. Veraenderungen, die sich auf den lateralen Anteil des Wirbelkoerpers, die Pars interarticularis und die kleinen Facettengelenke projizieren, koennen mit SPECT als spondylarthrotisch degenerative Laesionen eingestuft werden. Zwingende SPECT-szintigraphische Kriterien, die eine Laesion als eindeutig maligne nachweisen, gibt es dagegen nicht. Wir denken, dass SPECT bei allen Patienten mit Rueckenschmerzen und planarszintigraphisch suspekten

  19. Embryonic expression of Drosophila IMP in the developing CNS and PNS

    DEFF Research Database (Denmark)

    Adolph, Sidsel Kramshøj; Delotto, Robert; Nielsen, Finn Cilius;

    2008-01-01

    Drosophila IMP (dIMP) is related to the vertebrate RNA-binding proteins IMP1-3, ZBP1, Vg1RBP and CRD-BP, which are involved in RNA regulatory processes such as translational repression, localization and stabilization. The proteins are expressed in many fetal tissues, including the developing nerv...

  20. Clinical applications of SPECT-CT

    Energy Technology Data Exchange (ETDEWEB)

    Ahmadzadehfar, Hojjat; Biersack, Hans-Juergen (eds.) [University Hospital Bonn (Germany). Dept. of Nuclear Medicine

    2014-06-01

    Covers the full spectrum of clinical applications of SPECT/CT in diagnosis of benign and malignant diseases. Includes chapters on the use of SPECT/CT for dosimetry and for therapy planning. Completely up to date. Many helpful illustrations. SPECT/CT cameras have considerably improved diagnostic accuracy in recent years. Such cameras allow direct correlation of anatomic and functional information, resulting in better localization and definition of scintigraphic findings. In addition to this anatomic referencing, CT coregistration provides superior quantification of radiotracer uptake based on the attenuation correction capabilities of CT. Useful applications of SPECT/CT have been identified not only in oncology but also in other specialties such as orthopedics and cardiology. This book covers the full spectrum of clinical applications of SPECT/CT in diagnosis and therapy planning of benign and malignant diseases. Opening chapters discuss the technology and physics of SPECT/CT and its use for dosimetry. The role of SPECT/CT in the imaging of a range of pathologic conditions is then addressed in detail. Applications covered include, among others, imaging of the thyroid, bone, and lungs, imaging of neuroendocrine tumors, cardiac scintigraphy, and sentinel node scintigraphy. Individual chapters are also devoted to therapy planning in selective internal radiation therapy of liver tumors and bremsstrahlung SPECT/CT. Readers will find this book to be an essential and up-to-date source of information on this invaluable hybrid imaging technique.

  1. SPECT/CT and pulmonary embolism

    Energy Technology Data Exchange (ETDEWEB)

    Mortensen, Jann [Copenhagen University Hospital, Department of Clinical Physiology, Nuclear Medicine and PET, Rigshospitalet, Copenhagen (Denmark); The Faroese National Hospital, Department of Medicine, Torshavn (Faroe Islands); Gutte, Henrik [Copenhagen University Hospital, Department of Clinical Physiology, Nuclear Medicine and PET, Rigshospitalet, Copenhagen (Denmark); Herlev Hospital, Copenhagen University Hospital, Department of Radiology, Copenhagen (Denmark); University of Copenhagen, Cluster for Molecular Imaging, Faculty of Health Sciences, Copenhagen (Denmark)

    2014-05-15

    Acute pulmonary embolism (PE) is diagnosed either by ventilation/perfusion (V/P) scintigraphy or pulmonary CT angiography (CTPA). In recent years both techniques have improved. Many nuclear medicine centres have adopted the single photon emission CT (SPECT) technique as opposed to the planar technique for diagnosing PE. SPECT has been shown to have fewer indeterminate results and a higher diagnostic value. The latest improvement is the combination of a low-dose CT scan with a V/P SPECT scan in a hybrid tomograph. In a study comparing CTPA, planar scintigraphy and SPECT alone, SPECT/CT had the best diagnostic accuracy for PE. In addition, recent developments in the CTPA technique have made it possible to image the pulmonary arteries of the lungs in one breath-hold. This development is based on the change from a single-detector to multidetector CT technology with an increase in volume coverage per rotation and faster rotation. Furthermore, the dual energy CT technique is a promising modality that can provide functional imaging in combination with anatomical information. Newer high-end CT scanners and SPECT systems are able to visualize smaller subsegmental emboli. However, consensus is lacking regarding the clinical impact and treatment. In the present review, SPECT and SPECT in combination with low-dose CT, CTPA and dual energy CT are discussed in the context of diagnosing PE. (orig.)

  2. SPECT/CT and pulmonary embolism

    DEFF Research Database (Denmark)

    Mortensen, Jann; Borgwardt, Henrik Gutte

    2014-01-01

    Acute pulmonary embolism (PE) is diagnosed either by ventilation/perfusion (V/P) scintigraphy or pulmonary CT angiography (CTPA). In recent years both techniques have improved. Many nuclear medicine centres have adopted the single photon emission CT (SPECT) technique as opposed to the planar...... technique for diagnosing PE. SPECT has been shown to have fewer indeterminate results and a higher diagnostic value. The latest improvement is the combination of a low-dose CT scan with a V/P SPECT scan in a hybrid tomograph. In a study comparing CTPA, planar scintigraphy and SPECT alone, SPECT/CT had...... the best diagnostic accuracy for PE. In addition, recent developments in the CTPA technique have made it possible to image the pulmonary arteries of the lungs in one breath-hold. This development is based on the change from a single-detector to multidetector CT technology with an increase in volume...

  3. Significance of IMP3, nucleophosmin, and Ki-67 expression in papillary thyroid carcinoma.

    Science.gov (United States)

    Yorukoglu, Aygun; Yalcin, Nagihan; Avci, Arzu; Cakalagaoglu, Fulya; Yaylali, Guzin; Akin, Fulya; Haciyanli, Mehmet; Ozden, Akin

    2015-02-01

    The purpose of our study was to investigate the diagnostic value of expression of IMP3, nucleophosmin, and correlation of these markers with Ki-67 proliferation index in papillary thyroid carcinoma and benign neoplasms of thyroid gland. The aim was also to investigate whether there is a difference between papillary and micropapillary carcinomas with regard to clinicopathologic parameters beside IMP3, nucleophosmin, and Ki-67 proliferation index. It was concluded that IMP3 and nucleophosmin cannot be a routine diagnostic marker for discrimination of papillary carcinomas and benign lesions. IMP3 positive staining was quite scarce in IMP3 positive papillary carcinomas although specifity of IMP3 is 100%. A statistically significant correlation was not detected between nucleophosmin, IMP-3, and Ki-67 proliferation index. A statistically significant correlation was found between tumor size, lymphovascular embolism, and Ki-67 proliferation index. There was also significant correlation between tumor size and lymphovascular embolism.

  4. Molecular imaging of angiogenesis with SPECT

    Energy Technology Data Exchange (ETDEWEB)

    Dijkgraaf, Ingrid; Boerman, Otto C. [Radboud University Nijmegen Medical Center, Department of Nuclear Medicine, P.O. Box 9101, HB Nijmegen (Netherlands)

    2010-08-15

    Single-photon emission computed tomography (SPECT) and position emission tomography (PET) are the two main imaging modalities in nuclear medicine. SPECT imaging is more widely available than PET imaging and the radionuclides used for SPECT are easier to prepare and usually have a longer half-life than those used for PET. In addition, SPECT is a less expensive technique than PET. Commonly used gamma emitters are: {sup 99m}Tc (E{sub max} 141 keV, T{sub 1/2} 6.02 h), {sup 123}I (E{sub max} 529 keV, T{sub 1/2} 13.0 h) and {sup 111}In (E{sub max} 245 keV, T{sub 1/2} 67.2 h). Compared to clinical SPECT, PET has a higher spatial resolution and the possibility to more accurately estimate the in vivo concentration of a tracer. In preclinical imaging, the situation is quite different. The resolution of microSPECT cameras (<0.5 mm) is higher than that of microPET cameras (>1.5 mm). In this report, studies on new radiolabelled tracers for SPECT imaging of angiogenesis in tumours are reviewed. (orig.)

  5. Stratification of cerebral hemodynamics of child moyamoya disease using CBF SPECT

    Energy Technology Data Exchange (ETDEWEB)

    Nakagawara, Jyoji; Saito, Norihiro; Osato, Toshiaki; Kamiyama, Kenji; Takeda, Rihei; Nakamura, Hirohiko [Nakamura Memorial Hospital, Sapporo (Japan)

    2002-12-01

    In order to make sure the stratification of cerebral hemodynamics of child moyamoya disease, we evaluated {sup 123}I-IMP SPECT before and after surgical revascularization. The aim of this paper is to establish the semiquantitative parameters that can be applied to estimate severity of hemodynamic cerebral ischemia instead of quantitative parameters. Quantitative studies using IMP-autoradiography (ARG) method were performed on thirteen patients to measure resting regional cerebral blood flow (rCBF), and vascular reserve (rVR): (Diamox-activated rCBF/resting rCBF-1) x 100%. Semiquantitative parameters were calculated from the ratio of region of interest (ROI) counts in the anterior cerebral artery (ACA) and middle cerebral artery (MCA) territory to the dominant cerebellum counts (ROI/Ce ratio) at resting and Diamox-activated conditions. From the quantitative study, both mean resting rCBF less than 40 ml/100 g/min and rVR less than 10% could indicate stage 2 hemodynamic cerebral ischemia. The prediction of stage 2 ischemia using semiquantitative parameters (resting ROI/Ce ratio less than 0.9 and Diamox-activated ROI/Ce ratio less than 0.85 in the MCA territory) was not statistically different comparison to the diagnosis using quantitative parameters (using Fisher exact test<0.0001, Sensitivity and specificity were 87.5% and 90.9%, respectively). The ROI/Ce ratio can be utilized as simple parameters instead of quantitative parameters. (author)

  6. Validation and optimization of the use of standardized arterial input function in N-isopropyl-p[{sup 123}I]iodoamphetamine cerebral blood flow SPECT

    Energy Technology Data Exchange (ETDEWEB)

    Ogura, Toshiyuki; Takikawa, Syugo; Saito, Hisatoshi [Sapporo Azabu Neurosurgical Hospital (Japan); Nakazawa, Mayumi; Shidahara, Miho; Iida, Hidehiro

    1999-10-01

    Use of a standardized arterial input function and calibrating it by a single blood sample has been proposed to assess quantitatively cerebral blood flow using N-isopropyl-p[{sup 123}I]iodoamphetamine (IMP) and single-photon emission computed tomography. This study was intended to validate this approach using a larger number of measured arterial radioactivity curves in the clinical setting. Arterial input function was measured for 50 patients at rest following the i.v. IMP, and its inter-subject variation was assessed. Difference between smokers and non-smokers in addition to effects of acetazolamide administration were particularly investigated. We also evaluated the accuracy of the calibration procedures by means of either a single blood sample or a continuous arterial blood withdrawal sampling for an early period. Inter-subject variation of the observed arterial input function appeared not to show large variations among the 50 patients, thus suggesting the validity of using the standardized arterial input function for the IMP SPECT study. There was a significant difference in the shape of the arterial input function between the smokers and non-smokers, but the calibration at an optimized sampling time provided the area-under-the curve (AUC) that was not significantly different between the two groups. The arterial input function after the acetazolamide showed no significant difference as compared with the shape at rest. The calibration of the standardized input function by means of the early integration of individual curve did not show better accuracy except for a short period of AUC (i.e., <20 min) for longer integration period >10 min. Thus, use of the standardized arterial input function has been validated for the IMP SPECT study. The single blood sampling procedure for calibrating the standardized input function has also been validated, and has been shown to provide better accuracy compared with the continuous withdrawal procedure.(author)

  7. Crossed cerebellar hyperperfusion in brain perfusion SPECT

    Energy Technology Data Exchange (ETDEWEB)

    Jinnouchi, Seishi; Nagamachi, Shigeki; Nishii, Ryuuichi; Futami, Shigemi; Tamura, Shozo [Miyazaki Medical Coll., Kiyotake (Japan); Kawai, Keiichi

    2000-10-01

    Crossed cerebellar diaschisis is a well-known brain SPECT finding in stroke patients. Few reports, however, have described supratentorial and contralateral cerebellar hyperperfusion (crossed cerebellar hyperperfusion, CCH). We assessed the incidence of CCH in 33 patients with cerebral hyperperfusion. Brain SPECT showed CCH in five patients out of 20 epilepsy and three of 13 patients with acute encephalitis. These eight patients with CCH had recent epileptic attack. CCH was found in ECD SPECT as well as HM-PAO. The contralateral cerebellar activity correlated with the cerebral activity in patients with CCH. CCH would have a relation with supratentrial hyperfunction in epilepsy and acute encephalitis. (author)

  8. SPECT Myocardial Blood Flow Quantitation Concludes Equivocal Myocardial Perfusion SPECT Studies to Increase Diagnostic Benefits.

    Science.gov (United States)

    Chen, Lung-Ching; Lin, Chih-Yuan; Chen, Ing-Jou; Ku, Chi-Tai; Chen, Yen-Kung; Hsu, Bailing

    2016-01-01

    Recently, myocardial blood flow quantitation with dynamic SPECT/CT has been reported to enhance the detection of coronary artery disease in human. This advance has created important clinical applications to coronary artery disease diagnosis and management for areas where myocardial perfusion PET tracers are not available. We present 2 clinical cases that undergone a combined test of 1-day rest/dipyridamole-stress dynamic SPECT and ECG-gated myocardial perfusion SPECT scans using an integrated imaging protocol and demonstrate that flow parameters are capable to conclude equivocal myocardial perfusion SPECT studies, therefore increasing diagnostic benefits to add value in making clinical decisions.

  9. Benzodiazepine receptor imaging with iomazenil SPECT in aphasic patients with cerebral infarction

    Energy Technology Data Exchange (ETDEWEB)

    Koshi, Yasuhiko; Kitamura, Shin; Ohyama, Masashi [Nippon Medical School, Tokyo (Japan)] (and others)

    1999-08-01

    To investigate the relationship between prognosis of aphasia and neuronal damage in the cerebral cortex, we evaluated the distribution of central-type benzodiazepine receptor (BZR) binding in post-stroke aphasics with [{sup 123}I]iomazenil and SPECT. We performed iomazenil SPECT in six aphasic patients (aged from 45 to 75 years; all right-handed) with unilateral left cerebral infarction. Three patients showed signs of Broca's aphasia and the other three Wernicke's aphasia. Cerebral blood flow (CBF) imaging was performed with [{sup 123}I]iodoamphetamine (IMP). The regions of interest (ROIs) on both images were set in the cerebral cortex, cerebellar cortex and language relevant area in both hemispheres. Three patients were classified in the mild prognosis group and the other three in the moderate prognosis group. The left language-relevant area was more closely concerned with the difference in aphasic symptoms than the right one in both BZR and CBF distribution, but the ipsilateral to the contralateral ratio (I/C ratio) in the language-relevant areas in the BZR distribution was significantly lower in the moderate prognosis group than in the mild prognosis group, although no difference was seen for these values between the two groups in the CBF distribution. These results suggest that BZR imaging, which makes possible an increase in neuronal cell viability in the cerebral cortex, is useful not only for clarifying the aphasic symptoms but also for evaluating the prognosis of aphasia in patients with cerebral infarction. (author)

  10. Detection of misery perfusion in the cerebral hemisphere with chronic unilateral major cerebral artery steno-occlusive disease using crossed cerebellar hypoperfusion: comparison of brain SPECT and PET imaging

    Energy Technology Data Exchange (ETDEWEB)

    Matsumoto, Yoshiyasu; Ogasawara, Kuniaki; Saito, Hideo; Takahashi, Yoshihiro; Ogasawara, Yasushi; Kobayashi, Masakazu; Ogawa, Akira [Iwate Medical University, Department of Neurosurgery, Morioka (Japan); Iwate Medical University, Cyclotron Research Center, Morioka (Japan); Terasaki, Kazunori [Iwate Medical University, Cyclotron Research Center, Morioka (Japan); Yoshida, Kenji; Beppu, Takaaki; Kubo, Yoshitaka; Fujiwara, Shunrou [Iwate Medical University, Department of Neurosurgery, Morioka (Japan); Tsushima, Eiki [Hirosaki University, Graduate School of Health Sciences, Hirosaki (Japan)

    2013-10-15

    In patients with unilateral internal carotid or middle cerebral artery (ICA or MCA) occlusive disease, the degree of crossed cerebellar hypoperfusion that is evident within a few months after the onset of stroke may reflect cerebral metabolic rate of oxygen in the affected cerebral hemisphere relative to that in the contralateral cerebral hemisphere. The aim of the present study was to determine whether the ratio of blood flow asymmetry in the cerebellar hemisphere to blood flow asymmetry in the cerebral hemisphere on positron emission tomography (PET) and single photon emission computed tomography (SPECT) correlates with oxygen extraction fraction (OEF) asymmetry in the cerebral hemisphere on PET in patients with chronic unilateral ICA or MCA occlusive disease and whether this blood flow ratio on SPECT detects misery perfusion in the affected cerebral hemisphere in such patients. Brain blood flow and OEF were assessed using {sup 15}O-PET and N-isopropyl-p-[{sup 123}I]iodoamphetamine ({sup 123}I-IMP) SPECT, respectively. All images were anatomically standardized using SPM2. A region of interest (ROI) was automatically placed in the bilateral MCA territories and in the bilateral cerebellar hemispheres using a three-dimensional stereotaxic ROI template, and affected-to-contralateral asymmetry in the MCA territory or contralateral-to-affected asymmetry in the cerebellar hemisphere was calculated. Sixty-three patients with reduced blood flow in the affected cerebral hemisphere on {sup 123}I-IMP SPECT were enrolled in this study. A significant correlation was observed between MCA ROI asymmetry of PET OEF and the ratio of cerebellar hemisphere asymmetry of blood flow to MCA ROI asymmetry of blood flow on PET (r = 0.381, p = 0.0019) or SPECT (r = 0.459, p = 0.0001). The correlation coefficient was higher when reanalyzed in a subgroup of 43 patients undergoing a PET study within 3 months after the last ischemic event (r = 0.541, p = 0.0001 for PET; r = 0.609, p < 0

  11. Iodine-123 labeled derivatives of methylphenidate: potential SPECT radiopharmaceuticals for brain dopamine transporters

    Energy Technology Data Exchange (ETDEWEB)

    Pan, D.; Gatley, S.J.; Chen, R.; Ding, Y.-S. [Brookhaven National Lab., Upton, NY (United States)

    1996-06-01

    Since dl-threo-[{sup 11}C]methylphenidate (Ritalin) and especially the more active enantiomer, d-threo-[{sup 11}C]methylphenidate, have favorable properties for PET studies, we prepared two radioiodinated analogs of methylphenidate, p-[{sup 123}I]iodomethylphenidate and m-[{sup 123}I]iodo-p-hydroxymethylphenidate with a view to evaluating them as potential SPECT tracers. To prepare p-[{sup 123}I]iodomethylphenidate, the p-tributyltin derivative was prepared from the previously reported p-bromomethylphenidate and reacted under acidic conditions with I-123 iodide plus chloramine-T at room temperature for 90 seconds. The predomimant radioactive product was obtained in 85% radiochemical yield and > 10 Ci/{mu}mol specific radioactivity after HPLC purification. It had the same HPLC retention time as a spectroscopically characterized non-radioactive p-iodomethylphenidate standard prepared via nitration of methylphenidate and diazotization, after protection of the secondary amino group by benzoylation. A second radioiodinated methylphenidate derivative, m-[{sup 123}I]iodop-hydroxymethylphenidate was prepared in 80% radiochemical yield by direct iodination of the known p-hydroxymethylphenidate. In this case the non-radioactive standard was prepared by iodination of p-hydroxyritalinic acid using I{sub 2} and iodic acid, followed by esterification. (author).

  12. Characterization of a novel blaIMP gene, blaIMP-58, using whole genome sequencing in a Pseudomonas putida isolate detected in Denmark

    DEFF Research Database (Denmark)

    Holmgaard, Dennis Back; Hansen, Frank; Hasman, Henrik

    2017-01-01

    A multidrug-resistant strain of Pseudomonas putida was isolated from the urine of a 65-year-old women hospitalized for serious clinical conditions. Using whole genome sequencing a novel blaIMP gene, blaIMP-58 was discovered and characterized....

  13. IMP3 RNP safe houses prevent miRNA-directed HMGA2 mRNA decay in cancer and development

    DEFF Research Database (Denmark)

    Jønson, Lars; Christiansen, Jan; Hansen, Thomas van Overeem;

    2014-01-01

    The IMP3 RNA-binding protein is associated with metastasis and poor outcome in human cancer. Using solid cancer transcriptome data, we found that IMP3 correlates with HMGA2 mRNA expression. Cytoplasmic IMP3 granules contain HMGA2, and IMP3 dose-dependently increases HMGA2 mRNA. HMGA2 is regulated...... that IMP3 RNPs may function as cytoplasmic safe houses and prevent miRNA-directed mRNA decay of oncogenes during tumor progression....

  14. p62/IMP2 stimulates cell migration and reduces cell adhesion in breast cancer

    Science.gov (United States)

    Li, Yang; Francia, Giulio; Zhang, Jian-Ying

    2015-01-01

    p62/IMP2 is an oncofetal protein that is overexpressed in several types of cancer, and is a member of the family of insulin-like growth factor 2 mRNA binding proteins. We previously reported that high levels of p62/IMP2 autoantibody are present in sera from cancer patients, compared to healthy individuals. Here, we report the overexpression of p62/IMP2 in tumor tissues of 72 out of 104 cases of human breast cancer, and high levels of p62/IMP2 autoantibody in patients’ sera (in 63 out of 216 cases). To explore the role of p62/IMP2 in breast cancer progression, we generated p62/IMP2 transfected variants of two human breast cancer cell lines: MDA-MB-231 and LM2-4. Using in vitro assays we found that overexpression of p62/IMP2 can increase cell migration, and reduce cell adhesion to extracellular matrix (ECM) proteins. A Human Extracellular Matrix and Adhesion Molecules qPCR array was performed with our generated variants, and it identified a group of mRNAs whose expression was altered with p62/IMP2 overexpression, including connective tissue growth factor (CTGF) mRNA – which we show to be a p62/IMP2 binding partner. Overall, our results provide new insights into the molecular mechanism by which p62/IMP2 can contribute to breast cancer progression. PMID:26416451

  15. Ictal SPECT in Sturge-Weber syndrome

    OpenAIRE

    O. Bilgin; Vollmar, C.; Peraud, A.; la Fougere, C.; Beleza, P; Noachtar, S.

    2008-01-01

    We report on a patient with right-sided Sturge-Weber syndrome (SWS), in whom earlier functional hemispherectomy failed. Subtraction of ictal and interictal single-photon-emission-computed-tomography (SPECT) superimposed on individual MRI showed a right fronto-orbital hyperperfusion, with a left-sided EEG seizure pattern. Ictal SPECT supported our assumption that right frontal originated seizure pattern propagated to left hemisphere via the remaining right frontal bridge. Right orbito-frontal ...

  16. Computer-assisted diagnostic system for neurodegenerative dementia using brain SPECT and 3D-SSP

    Energy Technology Data Exchange (ETDEWEB)

    Ishii, Kazunari; Kanda, Tomonori; Uemura, Takafumi; Miyamoto, Naokazu; Yoshikawa, Toshiki [Hyogo Brain and Heart Center, Department of Radiology and Nuclear Medicine, Himeji, Hyogo (Japan); Shimada, Kenichi; Ohkawa, Shingo [Hyogo Brain and Heart Center, Institute for Aging Brain and Cognitive Disorders, Himeji, Hyogo (Japan); Minoshima, Satoshi [University of Washington, Radiology and Bioengineering, Department of Radiology, Seattle, WA (United States)

    2009-05-15

    To develop a computer-assisted automated diagnostic system to distinguish among Alzheimer disease (AD), dementia with Lewy bodies (DLB), and other degenerative disorders in patients with mild dementia. Single photon emission computed tomography (SPECT) images with injection of N-Isopropyl-p-[{sup 123}I]iodoamphetamine (IMP) were obtained from patients with mild degenerative dementia. First, datasets from 20 patients mild AD, 15 patients with dementia with DLB, and 17 healthy controls were used to develop an automated diagnosing system based on three-dimensional stereotactic surface projections (3D-SSP). AD- and DLB-specific regional templates were created using 3D-SSP, and critical Z scores in the templates were established. Datasets from 50 AD patients, 8 DLB patients, and 10 patients with non-AD/DLB type degenerative dementia (5 with frontotemporal dementia and 5 with progressive supranuclear palsy) were then used to test the diagnostic accuracy of the optimized automated system in comparison to the diagnostic interpretation of conventional IMP-SPECT images. These comparisons were performed to differentiate AD and DLB from non-AD/DLB and to distinguish AD from DLB. A receiver operating characteristic (ROC) analysis was performed. The area under the ROC curve (Az) and the accuracy of the automated diagnosis system were 0.89 and 82%, respectively, for AD/DLB vs. non-AD/DLB patients, and 0.70 and 65%, respectively, for AD vs. DLB patients. The mean Az and the accuracy of the visual inspection were 0.84 and 77%, respectively, for AD/DLB vs. non-AD/DLB patients, and 0.70 and 65%, respectively, for AD vs. DLB patients. The mean Az and the accuracy of the combination of visual inspection and this system were 0.96 and 91%, respectively, for AD/DLB vs. non-AD/DLB patients, and 0.70 and 66%, respectively, for AD vs. DLB patients. The system developed in the present study achieved as good discrimination of AD, DLB, and other degenerative disorders in patients with mild

  17. Automatic composition of MRI and SPECT images

    Energy Technology Data Exchange (ETDEWEB)

    Nishimura, Hiromi [Research Inst. of Brain and Blood Vessels, Akita (Japan)

    1999-12-01

    The new method to automatically compose MRI image and SPECT image was devised to support the SPECT image which was inferior in the morphological information. This method is a kind of the coordinate transformation to obtain maximal agreement between images using cross correlation of MRI image and SPECT image as the evaluation function to show the degree of the agreement. For the calculation of the cross correlation, MRI T1 weighted image and the morphological information of SPECT image treated by the spatial quadratic differentiation (Laplacian) were used. This method does not require to fix the control point in the tomographic imaging, and can be also applied to PET other than SPECT. This is also useful to follow up the chronological change of a patient by composition among SPECT images and among PET images. Since this method is focused on the internal structure of brain, it is also useful for cases such as cerebral infarction which brain structure has little change. But this method is still under the trial and the examination of the accuracy remained. (K.H.)

  18. SPECT/CT diagnostics for skeletal infections; SPECT/CT-Infektdiagnostik am Skelett

    Energy Technology Data Exchange (ETDEWEB)

    Klaeser, B.; Spanjol, M.; Krause, T. [Inselspital Bern, Universitaetsklinik fuer Nuklearmedizin, Bern (Switzerland)

    2012-07-15

    Skeletal infections are often a diagnostic and clinical challenge. Nuclear imaging modalities used in the diagnostic workup of acute and chronic skeletal infections include three-phase bone scintigraphy and scintigraphy with labelled leucocytes. The introduction of hybrid technologies, such as single photon emission computed tomography/computed tomography (SPECT/CT) has dramatically changed nuclear medical imaging of infections. In general SPECT/CT leads to a considerably more accurate diagnosis than planar or SPECT imaging. Given the integrated acquisition of metabolic, functional and morphological information, SPECT/CT has increased in particular the specificity of three-phase skeletal scanning and scintigraphy with labeled leucocytes. (orig.) [German] Knoecherne Infekte stellen nicht selten eine diagnostische und klinische Herausforderung dar. Nuklearmedizinische Standardverfahren fuer die Diagnostik akuter und chronischer Knocheninfekte sind die Mehrphasenskelettszintigraphie und die Infektszintigraphie mit markierten Leukozyten. Die Einfuehrung von Hybridtechnologien wie der SPECT/CT hat die nuklearmedizinische Infektbildgebung tiefgreifend veraendert. Die SPECT/CT erlaubt bei der Frage nach Knocheninfekten insgesamt eine wesentlich genauere Beurteilung als planare Aufnahmen und SPECT. Die integrierte Akquisition von metabolischer, funktioneller und topographisch-morphologischer Information mit SPECT/CT steigerte insbesondere die Spezifitaet der Mehrphasenskelettszintigraphie und der Infektszintigraphie mit markierten Leukozyten. (orig.)

  19. Clinical evaluation of stress thallium spect in ischemic heart disease

    Energy Technology Data Exchange (ETDEWEB)

    Sui, Osamu; Kimura, Nazuna; Soeki; Takeshi; Takeichi, Naoki; Shinohara, Hisanori; Tamura, Yoshiyuki; Fukuda, Nobuo [Zentsuji National Hospital, Kagawa (Japan)

    1997-05-01

    Thallium SPECT was performed in patients with significant coronary artery stenosis, 67 cases were after maximal exercise and 74 cases were during coronary vasodilation induced by ATP (adenosine triphosphate) infusion. In patients suspected of angina pectoris, the sensitivity, specificity and predictive accuracy for detection of coronary artery disease (CAD) were 88%, 78% and 82% for exercise SPECT, and 100%, 72% and 84% for ATP SPECT studies, respectively. In patients with old myocardial infarction, these were 73%, 100% and 88% for exercise SPECT and 71%, 100% and 81% for ATP SPECT. These were 75%, 49% and 60% for treadmill exercise test in the patient group including both angina and myocardial infarction. For detection of diseased vessels, the diagnostic accuracy for left anterior descending artery and right coronary artery lesions was almost equal for ATP and exercise SPECT study, but ATP SPECT study was more sensitive than exercise SPECT study in detection of left circumflex artery lesions. ATP as well as exercise SPECT studies occasionally gave false positive results in patients with single-vessel disease. ATP as well as exercise SPECT studies underestimated the severity of multi-vessel disease. In general, the results of ATP SPECT imaging were highly concordant with the results of exercise SPECT imaging. ATP stress thallium SPECT imaging provided a safe and highly accurate diagnostic tool for detection of CAD. (author)

  20. SPECT/CT - Technical aspects and optimization possibilities; SPECT/CT - Technische Aspekte und Optimierungsmoeglichkeiten

    Energy Technology Data Exchange (ETDEWEB)

    Roemer, W. [Klinikum Passau, Klinik fuer Nuklearmedizin, Passau (Germany)

    2012-07-15

    In contrast to positron emission tomography/computed tomography (PET/CT), the currently available single photon emission computed tomography/computed tomography (SPECT/CT) systems are very heterogeneous. On the side of the gamma cameras, dual-head systems are established, which are not very different from one manufacturer to the other. For the CT component, there are low dose tubes on the one side and flat detector-based cone beam CT and multislice-CT on the other. The CT image data can be used for anatomic correlation of suspicious findings as well as for attenuation correction of SPECT data. Attenuation correction enables on the one hand enhancement of SPECT image quality and on the other hand quantification of the radioactivity concentration becomes possible. Modern iterative reconstruction algorithms allow scatter correction and attenuation correction of SPECT data using the density values from CT. It still has to be shown to what extent attenuation-corrected whole body SPECT/CT studies will be able to improve the sensitivity of scintigraphy studies. As SPECT/CT primarily aims at morphologic correlation and not detection of additional lesions, an attempt should be made to balance the necessary anatomic information and the additional radiation exposure. Besides SPECT-guided CT all technical possibilities for dose reduction should be exhausted. (orig.) [German] Im Gegensatz zur Positronenemissionstomographie/Computertomographie (PET/CT) sind die auf dem Markt angebotenen Single-photon-emission-computed-tomography/CT(SPECT/CT)-Systeme sehr heterogen. Auf der Seite der Gammakameras sind Zweikopfsysteme etabliert, die sich bei den verschiedenen Wettbewerbern nur unwesentlich unterscheiden. Hingegen reicht bei der CT-Komponente die Palette von der einfachen Niedrigdosisroentgenroehre ueber die flachdetektorbasierte Cone-beam-CT-Technologie bis hin zum Mehrzeilenspiral-CT. Die CT-Bilddaten werden dabei zum einen zur anatomischen Korrelation funktionell auffaelliger

  1. IMP-3 expression in keratoacanthomas and squamous cell carcinomas of the skin: an immunohistochemical study

    Science.gov (United States)

    Soddu, S.; Di Felice, E.; Cabras, S.; Castellanos, M.E.; Atzori, L.; Faa, G.; Pilloni, L.

    2013-01-01

    The protein insulin-like growth factor II mRNA binding protein 3 (IMP-3) is an important factor for cell migration and adhesion in malignancies. Recent studies have shown a remarkable overexpression of IMP-3 in different human malignant neoplasms and also revealed it as an important prognostic marker in some tumor entities. The purpose of this study is to compare IMP-3 immunostaining in cutaneous squamous cell tumors and determine whether IMP-3 can aid in the differential diagnosis of these lesions. To our knowledge, IMP-3 expression has not been investigated in skin squamous cell proliferations thus far. Immunohi-stochemical staining for IMP-3 was performed on slides organized by samples from 67 patients, 34 with keratoacanthoma (KA) and 33 with primary cutaneous squamous cell carcinoma (SCC) (16 invasive and 17 in situ). Seventyfour percent of KAs (25/34) were negative for IMP-3 staining, while 57% of SCCs (19/33) were positive for IMP-3 staining. The percentage of IMP-3 positive cells increased significantly in the invasive SCC group (P=0.0111), and particularly in the SCC in situ group (P=0.0021) with respect to the KA group. IMP-3 intensity staining was significantly higher in invasive SCCs (P=0.0213), and particularly in SCCs in situ (P=0.008) with respect to KA. Our data show that IMP-3 expression is different in keratoacanthoma with respect to squamous cell carcinoma. IMP-3 assessment and staining pattern, together with a careful histological study, can be useful in the differential diagnosis between KA e SCC. PMID:23549465

  2. SPECT/CT workflow and imaging protocols

    Energy Technology Data Exchange (ETDEWEB)

    Beckers, Catherine [University Hospital of Liege, Division of Nuclear Medicine and Oncological Imaging, Department of Medical Physics, Liege (Belgium); Hustinx, Roland [University Hospital of Liege, Division of Nuclear Medicine and Oncological Imaging, Department of Medical Physics, Liege (Belgium); Domaine Universitaire du Sart Tilman, Service de Medecine Nucleaire et Imagerie Oncologique, CHU de Liege, Liege (Belgium)

    2014-05-15

    Introducing a hybrid imaging method such as single photon emission computed tomography (SPECT)/CT greatly alters the routine in the nuclear medicine department. It requires designing new workflow processes and the revision of original scheduling process and imaging protocols. In addition, the imaging protocol should be adapted for each individual patient, so that performing CT is fully justified and the CT procedure is fully tailored to address the clinical issue. Such refinements often occur before the procedure is started but may be required at some intermediate stage of the procedure. Furthermore, SPECT/CT leads in many instances to a new partnership with the radiology department. This article presents practical advice and highlights the key clinical elements which need to be considered to help understand the workflow process of SPECT/CT and optimise imaging protocols. The workflow process using SPECT/CT is complex in particular because of its bimodal character, the large spectrum of stakeholders, the multiplicity of their activities at various time points and the need for real-time decision-making. With help from analytical tools developed for quality assessment, the workflow process using SPECT/CT may be separated into related, but independent steps, each with its specific human and material resources to use as inputs or outputs. This helps identify factors that could contribute to failure in routine clinical practice. At each step of the process, practical aspects to optimise imaging procedure and protocols are developed. A decision-making algorithm for justifying each CT indication as well as the appropriateness of each CT protocol is the cornerstone of routine clinical practice using SPECT/CT. In conclusion, implementing hybrid SPECT/CT imaging requires new ways of working. It is highly rewarding from a clinical perspective, but it also proves to be a daily challenge in terms of management. (orig.)

  3. Indeterminate lesions on planar bone scintigraphy in lung cancer patients: SPECT, CT or SPECT-CT?

    Energy Technology Data Exchange (ETDEWEB)

    Sharma, Punit; Kumar, Rakesh; Singh, Harmandeep; Bal, Chandrasekhar; Malhotra, Arun [All India Institute of Medical Sciences, Department of Nuclear Medicine, New Delhi (India); Julka, Pramod Kumar [All India Institute of Medical Sciences, Department of Radiation Oncology, New Delhi (India); Thulkar, Sanjay [All India Institute of Medical Sciences, Department of Radiodiagnosis, New Delhi (India)

    2012-07-15

    The objective of the present study was to compare the role of single photon emission computed tomography (SPECT), computed tomography (CT) and SPECT-CT of selected volume in lung cancer patients with indeterminate lesions on planar bone scintigraphy (BS). The data of 50 lung cancer patients (53 {+-} 10.3 years; range 30-75; male/female 38/12) with 65 indeterminate lesions on planar BS (January 2010 to November 2010) were retrospectively evaluated. All of them underwent SPECT-CT of a selected volume. SPECT, CT and SPECT-CT images were independently evaluated by two experienced readers (experience in musculoskeletal imaging, including CT: 5 and 7 years) in separate sessions. A scoring scale of 1 to 5 was used, in which 1 is definitely metastatic, 2 is probably metastatic, 3 is indeterminate, 4 is probably benign and 5 is definitely benign. Sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) were calculated for each modality, taking a score {<=}2 as metastatic. With receiver operating characteristic (ROC) curve analysis, areas under the curve (AUC) were calculated for each modality and compared. Clinical and imaging follow-up and/or histopathology were taken as reference standard. For both readers SPECT was inferior to CT (P = 0.004, P = 0.022) and SPECT-CT (P = 0.003, P = 0.037). However, no significant difference was found between CT and SPECT-CT for reader 1 (P = 0.847) and reader 2 (P = 0.592). The findings were similar for lytic as well as sclerotic lesions. Moderate inter-observer agreement was seen for SPECT images ({kappa} = 0.426), while almost perfect agreement was seen for CT ({kappa} = 0.834) and SPECT-CT ({kappa} = 0.971). CT alone and SPECT-CT are better than SPECT for accurate characterisation of indeterminate lesions on planar BS in lung cancer patients. CT alone is not inferior to SPECT-CT for this purpose and might be preferred because of shorter acquisition time and wider availability. (orig.)

  4. Brain SPECT imaging in Sydenham's chorea

    Directory of Open Access Journals (Sweden)

    Barsottini O.G.P.

    2002-01-01

    Full Text Available The objective of the present study was to determine whether brain single-photon emission computed tomography (SPECT imaging is capable of detecting perfusional abnormalities. Ten Sydenham's chorea (SC patients, eight females and two males, 8 to 25 years of age (mean 13.4, with a clinical diagnosis of SC were submitted to brain SPECT imaging. We used HMPAO labeled with technetium-99m at a dose of 740 MBq. Six examinations revealed hyperperfusion of the basal ganglia, while the remaining four were normal. The six patients with abnormal results were females and their data were not correlated with severity of symptoms. Patients with abnormal brain SPECT had a more recent onset of symptoms (mean of 49 days compared to those with normal SPECT (mean of 85 days but this difference did not reach statistical significance. Brain SPECT can be a helpful method to determine abnormalities of the basal ganglia in SC patients but further studies on a larger number of patients are needed in order to detect the phase of the disease during which the examination is more sensitive.

  5. Reduction and analysis of data from the plasma wave instruments on the IMP-6 and IMP-8 spacecraft

    Science.gov (United States)

    Gurnett, D. A.; Anderson, R. R.

    1983-01-01

    The primary data reduction effort during the reporting period was to process summary plots of the IMP 8 plasma wave data and to submit these data to the National Space Science Data Center. Features of the electrostatic noise are compared with simultaneous observations of the magnetic field, plasma and energetic electrons. Spectral characteristics of the noise and the results of this comparison both suggest that in its high frequency part at least the noise does not belong to normal modes of plasma waves but represents either quasi-thermal noise in the non-Maxwellian plasma or artificial noise generated by spacecraft interaction with the medium.

  6. In vivo chemistry of iofetamine HCl iodine-123 (IMP)

    Energy Technology Data Exchange (ETDEWEB)

    Baldwin, R.M.; Wu, J.L.

    1988-01-01

    Application of chemical methods for characterizing the in vivo behavior of iofetamine HCI /sup 123/I (IMP) has shed light on the metabolism of iofetamine in animals and humans. A successful technique consists of ethyl acetate extraction of the metabolites from tissue samples acidified with perchloric acid, separation of the mixture by high performance liquid chromatography, and quantitation of the radioactive components with a sensitive scintillation detector. Metabolism of iofetamine HCI /sup 123/I proceeds sequentially from the N-isopropyl group on the amphetamine side chain. The first step, dealkylation to the primary amine p-iodoamphetamine (PIA), occurs readily in the brain, lungs, and liver; activity in the brain and lungs consists of only IMP and PIA even 24 hr after administration. The rate-limiting step appears to be deamination to give the transitory intermediate p-iodophenylacetone, which is rapidly degraded to p-iodobenzoic acid and conjugated with glycine in the liver to give the end product of metabolism, p-iodohippuric acid, which is excreted through the kidneys in the urine.

  7. The first interdisciplinary experiments at the IMP high energy microbeam

    Energy Technology Data Exchange (ETDEWEB)

    Du, Guanghua; Guo, Jinlong; Wu, Ruqun; Guo, Na; Liu, Wenjing; Ye, Fei; Sheng, Lina; Li, Qiang [Institute of Modern Physics (IMP), Chinese Academy of Sciences, Lanzhou (China); Li, Huiyun [Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen (China)

    2015-04-01

    The high energy beam of tens to hundred MeV/u ions possesses mm-to-cm penetration depth in materials and can be easily extracted into air without significant energy loss and beam scattering. Combination of high energy ions and microbeam technology facilitates the microprobe application to many practical studies in large scale samples. The IMP heavy ion microbeam facility has recently been integrated with microscopic positioning and targeting irradiation system. This paper introduced the first interdisciplinary experiments performed at the IMP microbeam facility using the beam of 80.5 MeV/u carbon ions. Bystander effect induction via medium transferring was not found in the micro-irradiation study using HeLa cells. The mouse irradiation experiment demonstrated that carbon irradiation of 10 Gy dose to its tuberomammillary nucleus did not impair the sleep nerve system. The fault injection attack on RSA (Rivest–Shamir–Adleman) decryption proved that the commercial field-programmable gate array chip is vulnerable in single event effect to low linear-energy-transfer carbon irradiation, and the attack can cause the leakage of RSA private key. This work demonstrates the potential of high energy microbeam in its application to biology, biomedical, radiation hardness, and information security studies.

  8. Comparison of (123)I-MIBG myocardial scintigraphy, brain perfusion SPECT, and voxel-based MRI morphometry for distinguishing between dementia with Lewy bodies and Alzheimer's disease.

    Science.gov (United States)

    Inui, Yoshitaka; Toyama, Hiroshi; Manabe, Yuta; Sarai, Masayoshi; Iwata, Nakao

    2014-10-01

    This study aimed to compare the diagnostic value of (123)I-metaiodobenzylguanidine (MIBG) myocardial scintigraphy, N-isopropyl-p[(123)I]iodoamphetamine (IMP) brain perfusion single-photon emission computed tomography (SPECT), and brain magnetic resonance imaging (MRI) voxel-based morphometry (VBM) for the differentiation of dementia with Lewy bodies (DLB) and Alzheimer's disease (AD). Thirty-five and 34 patients with probable DLB and probable AD, respectively, were enrolled. All patients underwent (123)I-MIBG myocardial scintigraphy, (123)I-IMP brain perfusion SPECT, and brain MRI. For (123)I-MIBG imaging, we calculated early and delayed heart-to-mediastinum (H/M) uptake ratios. Three-dimensional stereotactic surface projections (3D-SSP) were used to analyze the results of (123)I-IMP SPECT. VBM with statistical parametric mapping 8 plus diffeomorphic anatomical registration using exponentiated Lie algebra (DARTEL) was used to analyze the brain MRI data. The area under the receiver operating characteristic curves (AUC) for discriminating DLB and AD was highest (0.882) for the delayed H/M ratio on (123)I-MIBG scintigraphy. AUC for z-score measurement in the occipital lobe was 0.818 and that for the extent of gray matter (GM) atrophy in the whole brain was 0.788. AUC for the combination of 3D-SSP and VBM analysis was 0.836. The respective sensitivities and specificities for distinguishing DLB from AD were 97.1 and 100 % for the delayed H/M ratio using (123)I-MIBG scintigraphy; 88.6 and 73.5 % for the occipital lobe z-score using 3D-SSP analysis; 85.7 and 64.7 % for the extent of whole brain GM atrophy using voxel-based MRI morphometry; and 91.4 and 76.5 % for the combination of 3D-SSP analysis and VBM. (123)I-MIBG myocardial scintigraphy was superior to brain perfusion SPECT and brain MRI using an advanced statistical technique to differentiate DLB and AD.

  9. SPECT/CT and tumour imaging

    Energy Technology Data Exchange (ETDEWEB)

    Abikhzer, Gad [Rambam Health Care Campus, Department of Nuclear Medicine, Haifa (Israel); Keidar, Zohar [Rambam Health Care Campus, Department of Nuclear Medicine, Haifa (Israel); Technion - Israel Institute of Technology, The Ruth and Bruce Rappaport Faculty of Medicine, Haifa (Israel)

    2014-05-15

    Scintigraphic techniques are sensitive imaging modalities in the diagnosis and follow-up of cancer patients providing the functional and metabolic activity characteristics of the tumour. Hybrid SPECT/CT improves the diagnostic accuracy of these well-established imaging techniques by precise anatomical localization and characterization of morphological findings, differentiation between foci of physiological and pathological tracer uptake, resulting in a significant impact on patient management and more definitive interpretations. The use of SPECT/CT has been studied in a variety of applications in tumour imaging which are reviewed in this article. By combining functional and anatomical information in a single imaging session, SPECT/CT has become a one-stop cancer imaging modality. (orig.)

  10. pIMP-PH114 carrying bla IMP-4 in a Klebsiella pneumoniae strain is closely related to other multidrug-resistant IncA/C2 plasmids.

    Science.gov (United States)

    Ho, Pak-Leung; Lo, Wai-U; Chan, Jane; Cheung, Yuk-Yam; Chow, Kin-Hung; Yam, Wing-Cheong; Lin, Chi-Ho; Que, Tak-Lun

    2014-02-01

    The IncA/C plasmids are broad host-range vehicles which have been associated with wide dissemination of CMY-2 among Enterobacteriaceae of human and animal origins. Acquired metallo-β-lactamases (MBLs) such as the IMP-type enzymes are increasingly reported in multidrug-resistant Gram-negative bacteria worldwide, particularly in Enterobacteriaceae. We described the complete sequence of the first IMP-4-encoding IncA/C2 plasmid, pIMP-PH114 (151,885 bp), from a sequence type 1 Klebsiella pneumoniae strain that was recovered from a patient who was hospitalized in the Philippines. pIMP-PH114 consists of a backbone from the IncA/C2 plasmids, with the insertion of a novel Tn21-like class 1 integron composite structure (containing the cassette array bla IMP-4-qacG-aacA4-catB3, followed by a class C β-lactamase bla DHA-1 and the mercury resistance operon, merRTPCADE) and a sul2-floR encoding region. Phylogenetic analysis of the IncA/C repA sequences showed that pIMP-PH114 formed a subgroup with other IncA/C plasmids involved in the international spread of CMY-2, TEM-24 and NDM-1. Identical bla IMP-4 arrays have been described among different Enterobacteriaceae and Acinetobacter spp. in China, Singapore and Australia but the genetic context is different. The broad host range of IncA/C plasmids may have facilitated dissemination of the bla IMP-4 arrays among different diverse groups of bacteria.

  11. IMP3 expression in human ovarian cancer is associated with improved survival

    DEFF Research Database (Denmark)

    Noske, Aurelia; Faggad, Areeg; Wirtz, Ralph

    2009-01-01

    The insulin-like growth factor-II mRNA-binding protein IMP3 plays an important role in embryogenesis and recent reports suggest an involvement in tumorigenesis. Although IMP3 expression has been well studied in mouse and human fetal and adult gonads, its role in ovarian cancer is unknown. We inve...

  12. Increased IMP dehydrogenase gene expression in solid tumor tissues and tumor cell lines

    Energy Technology Data Exchange (ETDEWEB)

    Collart, F.R.; Chubb, C.B.; Mirkin, B.L.; Huberman, E.

    1992-07-10

    IMP dehydrogenase, a regulatory enzyme of guanine nucleotide biosynthesis, may play a role in cell proliferation and malignancy. To assess this possibility, we examined IMP dehydrogenase expression in a series of human solid tumor tissues and tumor cell lines in comparison with their normal counterparts. Increased IMP dehydrogenase gene expression was observed in brain tumors relative to normal brain tissue and in sarcoma cells relative to normal fibroblasts. Similarly, in several B- and T-lymphoid leukemia cell lines, elevated levels of IMP dehydrogenase mRNA and cellular enzyme were observed in comparison with the levels in peripheral blood lymphocytes. These results are consistent with an association between increased IMP dehydrogenase expression and either enhanced cell proliferation or malignant transformation.

  13. Motor activation SPECT for the neurosurgical diseases. Clinical application

    Energy Technology Data Exchange (ETDEWEB)

    Noguchi, Hiroshi; Kawaguchi, Shoichiro; Sakaki, Toshisuke; Imai, Teruhiko; Ohishi, Hajime [Nara Medical Univ., Kashihara (Japan)

    1999-08-01

    We evaluated and analyzed the motor activation single photon emission computed tomography (M-SPECT) findings on patients with ischemic cerebrovascular diseases (CVD). The M-SPECT studies were carried out on 91 patients with ischemic cerebrovascular diseases. The M-SPECT study was performed using the finger opposition task in each case. The SPECT images were superimposed on the magnetic resonance images (MRIs) for each case using Image Fusion Software. The result of the M-SPECT was expressed as positive or negative. The cases with a marked increase of blood flow in the sensorio-motor cortex after the finger opposition task were categorized as positive, and those cases showing no marked increase of blood flow were categorized as negative. Among the 91 cases examined, 53 (58%) were categorized as positive in the M-SPECT study. Among the negative M-SPECT cases treated with revascularization surgery, there were some cases showing positive M-SPECT results postoperatively. The cases without any revascularization surgery did not change the M-SPECT findings in each during the follow-up period. The M-SPECT procedure for examining intracranial lesions could provide the cortical localization of the motor function. The M-SPECT procedure in the ischemic CVDs contributes to knowledge about the choices of treatment and the evaluation of the treatment result. (author)

  14. Application of SPECT to psychiatry

    Energy Technology Data Exchange (ETDEWEB)

    Matsuda, Hiroshi [National Center Hospital for Mental, Nervous, and Muscular Disorders, Kodaira, Tokyo (Japan)

    1999-07-01

    Brain perfusion SPECT using {sup 99m}Tc-ethyl-cysteinate dimer ({sup 99m}Tc-ECD) was applied to psychiatric diseases with aid of statistical parametric mapping (SPM) for analysis of data. To evaluate influence of aging on brain perfusion, noninvasive measurements of cerebral blood flow using {sup 99m}Tc-ECD were performed in 53 normal volunteers, age 18 to 87 years old. Mean cerebral blood flow (mCBF) was 43.9{+-}5.0 ml/100 g/min and showed weak negative correlation with aging (r=0.451). Perisylvian cerebral cortices and medial frontal areas including anterior cingulate gyri showed greater negative correlation than other areas. These findings suggest the necessity of age-matched control regional CBF (rCBF) data to investigate rCBF abnormality in patients. Four drug-naive schizophrenic patients showed flow decrease in bilateral frontal and superior temporal areas and a left infero-posterior temporal area. Haloperidol administration induced flow decrease in bilateral frontal and left parietal areas, while flow increase in bilateral striatal and right hippocampal areas. Ten aged depressive patients showed flow decrease in bilateral frontal and left temporo-parietal areas. Even after remission patients showed flow decrease in the left frontal area as compared with normal subjects. Remission induced flow increase in the right frontal, right parietal, and right orbitofrontal areas compared with depression. These results suggest that CBF measurements using {sup 99m}Tc-ECD were useful for objective evaluation of regional abnormality in brain function in psychiatric diseases. (author)

  15. IMP3 expression in biopsy specimens of colorectal cancer predicts lymph node metastasis and TNM stage.

    Science.gov (United States)

    Wei, Qingzhu; Huang, Xiaoping; Fu, Bo; Liu, Jianghuan; Zhong, Ling; Yang, Qiao; Zhao, Tong

    2015-01-01

    IMP3 is associated with lymph node metastasis and TNM stage and is a good independent prognostic biomarker for colorectal cancer (CRC). However, the expression status and clinical implication of IMP3 in biopsy specimens have not yet been studied. We aim to address whether the presence of IMP3 expression in preoperative biopsies of CRC could predict lymph node metastasis and TNM stage. In this study, we examined IMP3 expression in paired biopsy and resection specimens of 71 CRC and analyzed the correlation of IMP3 expression with clinicopathological parameters. In the biopsy specimens, IMP3 positive expression was observed in 56 of 71 cases (78.9%) whereas negative expression was observed in 15 of 71 cases (21.1%). In the resection specimens, IMP3 positive expression was detected in 83.1% cases (59/71) whereas negative expression was detected in 16.9% cases (12/71). The absolute concordance rate between biopsy and resection specimens was 90.1% (64/71). The Spearman correlation test documented the existence of a strong linear correlation between the percentage of IMP3-positive cells in the biopsy and resection specimen (r = 0.629; P TNM stage (P = 0.007), tumor border (P = 0.049) and tumor budding (P = 0.012). IMP3 expression in biopsy specimens was significantly related to lymph node metastasis (P = 0.004), TNM stage (P = 0.005) and tumor budding (P = 0.001). In conclusion, IMP3 expression in biopsy specimens could be used to predict lymph node metastasis and TNM stage in CRC patients.

  16. SPECT in psychiatry; Die Bedeutung der Hirn-SPECT in der Psychiatrie

    Energy Technology Data Exchange (ETDEWEB)

    Kasper, S. [Universitaetsklinik fuer Psychiatrie, Wien (Austria); Gruenwald, F. [Bonn Univ. (Germany). Klinik fuer Nuklearmedizin; Danos, P. [Psychiatrische Universitaetsklinik, Bonn (Germany); Walter, H. [Universitaetsklinik fuer Psychiatrie, Wien (Austria); Klemm, E. [Bonn Univ. (Germany). Klinik fuer Nuklearmedizin; Bruecke, T. [Universitaetsklinik fuer Neurologie, Wien (Austria); Podreka, I. [Universitaetsklinik fuer Neurologie, Wien (Austria); Biersack, H.J. [Bonn Univ. (Germany). Klinik fuer Nuklearmedizin

    1994-10-01

    In the last fifteen years different attempts have been undertaken to understand the biological basis of major psychiatric disorders. One important tool to determine patterns of brain dysfunction is single emission computed tomography (SPECT). Whereas SPECT investigations are already a valuable diagnostic instrument for the diagnosis of dementia of the Alzheimer Type (DAT) there have not been consistent findings that can be referred to as specific for any other particular psychiatric diagnostic entity. Nevertheless, SPECT studies have been able to demonstrate evidence of brain dysfunction in patients with schizophrenia, depression, anxiety disorders, and substance abuse in which other methods showed no clear abnormality of brain function. Our manuscript reviews the data which are currently available in the literature and stresses the need for further studies, especially for prediction and monitoring psychiatric treatment modalities. (orig.) [Deutsch] In den vergangenen 15 Jahren wurde durch verschiedene methodologische Ansaetze versucht, die biologischen Ursachen psychiatrischer Erkrankungen naeher zu erforschen. Als eine bedeutende Methode hat sich dabei die Single-Photonen-Emissions-Computertomographie (SPECT) herausgestellt. Waehrend die SPECT-Untersuchungen bereits Eingang in die Routinediagnostik bei Demenzen vom Alzheimer-Typ gefunden haben, konnten fuer weitere psychiatrische Erkrankungen noch keine eindeutigen Befunde etabliert werden. Mit der SPECT-Methode ist es jedoch gelungen, funktionelle Veraenderungen des Gehirns von psychiatrischen Erkrankungen darzustellen, wie z.B. Schizophrenie, Depression, Angsterkrankungen bzw. Substanzmissbrauch. In Forschungsprotokollen wird durch die SPECT-Methode versucht, abzuklaeren, inwieweit es moeglich ist, innerhalb der Erkrankungsentitaeten psychiatrischer Erkrankungen oder auch diese uebergreifend eine Subklassifizierung zu finden und evtl. Gehirnsysteme ausfindig zu machen, die mit einer spezifischen

  17. PET and SPECT in medically non-refractory complex partial seizures. Temporal asymmetries of glucose consumption, Benzodiazepine receptor density

    Energy Technology Data Exchange (ETDEWEB)

    Matheja, P.; Kuwert, T.; Wolf, K.; Schober, O. [Muenster Univ. (Germany). Kliniken und Polikliniken fuer Nuklearmedizin; Stodieck, S.R.G.; Diehl, B.; Ringelstein, E.B. [Muenster Univ. (Germany). Klinik fuer Neurologie; Schuierer, G. [Muenster Univ. (Germany). Inst. fuer Klinische Radiologie

    1998-12-31

    Aim: In contrast to medically refractory complex partial seizures (CPS), only limited knowledge exists on cerebral perfusion and metabolism in medically non-refractory CPS. The aim of this study was to investigate the frequency of temporal asymmetries in regional cerebral glucose consumption (rCMRGlc), regional cerebral blood flow (rCBF), and regional cerebral benzodiazepine receptor density (BRD) in this group of patients. Methods: The study included 49 patients with medically non-refractory cryptogenic CPS (age: 36.0{+-}16.1 years). rCMRGlc was studied with F-18-FDG-PET (FDG), rCBF with Tc-99m-ECD-SPECT (ECD), and BRD with I-123-iomazenil-SPECT (IMZ). All studies were performed interictally and within four weeks in each patient. Duration of epilepsy ranged from 0.1 to 42 years (median 4.0 years). SPECT was performed with the triple-headed SPECT camera Multispect 3, PET with the PET camera ECAT EXACT 47. Using linear profiles, glucose consumption, as well as uptake of ECD and IMZ, were measured in four temporal regions of interest (ROIs), and asymmetry indices were calculated (ASY). The results were compared to 95% confidence intervals determined in control subjects. Results: Thirty-five of the 49 (71%) patients had at least one significantly elevated ASY; temporal rCMRGlc was asymmetrical in 41% of the patients, temporal BRD in 29%, and temporal rCBF in 24%. One patient had an asymmetry of all three variables, two of temporal rCMRGlc and BRD, three of temporal rCMRGlc and rCBF, and another four of rCBF and BRD. Fourteen patients had an isolated temporal asymmetry in rCMRGlc, seven in BRD, and four in rCBF. A discrepancy in lateralization between the three modalities was not observed. Conclusion: The majority of patients with medically non-refractory CPS have focal abnormalities of blood flow and metabolism in their temporal lobe. In this group of patients, FDG-PET demonstrates abnormalities with the highest frequency of the three modalities studied, followed by

  18. Performance evaluation of D-SPECT: a novel SPECT system for nuclear cardiology.

    Science.gov (United States)

    Erlandsson, Kjell; Kacperski, Krzysztof; van Gramberg, Dean; Hutton, Brian F

    2009-05-07

    D-SPECT (Spectrum Dynamics, Israel) is a novel SPECT system for cardiac perfusion studies. Based on CZT detectors, region-centric scanning, high-sensitivity collimators and resolution recovery, it offers potential advantages over conventional systems. A series of measurements were made on a beta-version D-SPECT system in order to evaluate its performance in terms of energy resolution, scatter fraction, sensitivity, count rate capability and resolution. Corresponding measurements were also done on a conventional SPECT system (CS) for comparison. The energy resolution of the D-SPECT system at 140 keV was 5.5% (CS: 9.25%), the scatter fraction 30% (CS: 34%), the planar sensitivity 398 s(-1) MBq(-1) per head ((99m)Tc, 10 cm) (CS: 72 s(-1) MBq(-1)), and the tomographic sensitivity in the heart region was in the range 647-1107 s(-1) MBq(-1) (CS: 141 s(-1) MBq(-1)). The count rate increased linearly with increasing activity up to 1.44 M s(-1). The intrinsic resolution was equal to the pixel size, 2.46 mm (CS: 3.8 mm). The average reconstructed resolution using the standard clinical filter was 12.5 mm (CS: 13.7 mm). The D-SPECT has superior sensitivity to that of a conventional system with similar spatial resolution. It also has excellent energy resolution and count rate characteristics, which should prove useful in dynamic and dual radionuclide studies.

  19. Performance evaluation of D-SPECT: a novel SPECT system for nuclear cardiology

    Science.gov (United States)

    Erlandsson, Kjell; Kacperski, Krzysztof; van Gramberg, Dean; Hutton, Brian F.

    2009-05-01

    D-SPECT (Spectrum Dynamics, Israel) is a novel SPECT system for cardiac perfusion studies. Based on CZT detectors, region-centric scanning, high-sensitivity collimators and resolution recovery, it offers potential advantages over conventional systems. A series of measurements were made on a β-version D-SPECT system in order to evaluate its performance in terms of energy resolution, scatter fraction, sensitivity, count rate capability and resolution. Corresponding measurements were also done on a conventional SPECT system (CS) for comparison. The energy resolution of the D-SPECT system at 140 keV was 5.5% (CS: 9.25%), the scatter fraction 30% (CS: 34%), the planar sensitivity 398 s-1 MBq-1 per head (99mTc, 10 cm) (CS: 72 s-1 MBq-1), and the tomographic sensitivity in the heart region was in the range 647-1107 s-1 MBq-1 (CS: 141 s-1 MBq-1). The count rate increased linearly with increasing activity up to 1.44 M s-1. The intrinsic resolution was equal to the pixel size, 2.46 mm (CS: 3.8 mm). The average reconstructed resolution using the standard clinical filter was 12.5 mm (CS: 13.7 mm). The D-SPECT has superior sensitivity to that of a conventional system with similar spatial resolution. It also has excellent energy resolution and count rate characteristics, which should prove useful in dynamic and dual radionuclide studies.

  20. Joseph Roth, tradutor do Império perdido

    Directory of Open Access Journals (Sweden)

    Luis S. Krausz

    2015-06-01

    Full Text Available Este artigo discute um folhetim jornalístico do escritor austríaco Joseph Roth que trata de uma miniatura do Templo de Salomão, encontrada por ele num café da Hirtenstrasse berlinense, no coração do que foi, na década de 1920, o bairro habitado pelos judeus do Leste da Europa na capital alemã. A nostalgia, sob cujo signo foi criada tal miniatura, torna-se uma metáfora da nostalgia pelo império Austro-Húngaro, chave para a compreensão da obra romanesca deste escritor. Ao mesmo tempo, esta nostalgia rothiana é contextualizada no âmbito das crenças místicas e messiânicas do judaísmo tradicional.

  1. PET and SPECT of neurobiological systems

    Energy Technology Data Exchange (ETDEWEB)

    Dierckx, Rudi A.J.O. [Groningen Univ. (Netherlands). Dept. of Nuclear Medicine and Molecular Imaging; Gent Univ. (Belgium). Dept. of Nuclear Medicine; Otte, Andreas [Univ. of Applied Sciences, Offenburg (Germany). Faculty of Electrical Engineering and Information Technology; Vries, Erik F.J. de; Waarde, Aren van (eds.) [Groningen Univ. (Netherlands). Dept. of Nuclear Medicine and Molecular Imaging

    2014-04-01

    Addresses a variety of aspects of neurotransmission in the brain. Details the latest results in probe development. Emphasis on a multidisciplinary approach. Written by internationally acclaimed experts. PET and SPECT of Neurobiological Systems combines the expertise of renowned authors whose dedication to the development of novel probes and techniques for the investigation of neurobiological systems has achieved international recognition. Various aspects of neurotransmission in the brain are discussed, such as visualization and quantification of (more than 20 different) neuroreceptors, neuroinflammatory markers, transporters, and enzymes as well as neurotransmitter synthesis, ?-amyloid deposition, cerebral blood flow, and the metabolic rate of glucose. The latest results in probe development are also detailed. Most chapters are written jointly by radiochemists and nuclear medicine specialists to ensure a multidisciplinary approach. This state of the art compendium will be valuable to anyone in the field of clinical or preclinical neuroscience, from the radiochemist and radiologist/nuclear medicine specialist to the interested neurobiologist and general practitioner. It is the second volume of a trilogy on PET and SPECT imaging in the neurosciences. Other volumes focus on PET and SPECT in psychiatry and PET and SPECT in neurology''.

  2. Methodology for ventilation/perfusion SPECT

    DEFF Research Database (Denmark)

    Bajc, Marika; Neilly, Brian; Miniati, Massimo

    2010-01-01

    Ventilation/perfusion single-photon emission computed tomography (V/Q SPECT) is the scintigraphic technique of choice for the diagnosis of pulmonary embolism and many other disorders that affect lung function. Data from recent ventilation studies show that the theoretic advantages of Technegas ov...

  3. Singular Value Decomposition of Pinhole SPECT Systems.

    Science.gov (United States)

    Palit, Robin; Kupinski, Matthew A; Barrett, Harrison H; Clarkson, Eric W; Aarsvold, John N; Volokh, Lana; Grobshtein, Yariv

    2009-03-12

    A single photon emission computed tomography (SPECT) imaging system can be modeled by a linear operator H that maps from object space to detector pixels in image space. The singular vectors and singular-value spectra of H provide useful tools for assessing system performance. The number of voxels used to discretize object space and the number of collection angles and pixels used to measure image space make the matrix dimensions H large. As a result, H must be stored sparsely which renders several conventional singular value decomposition (SVD) methods impractical. We used an iterative power methods SVD algorithm (Lanczos) designed to operate on very large sparsely stored matrices to calculate the singular vectors and singular-value spectra for two small animal pinhole SPECT imaging systems: FastSPECT II and M(3)R. The FastSPECT II system consisted of two rings of eight scintillation cameras each. The resulting dimensions of H were 68921 voxels by 97344 detector pixels. The M(3)R system is a four camera system that was reconfigured to measure image space using a single scintillation camera. The resulting dimensions of H were 50864 voxels by 6241 detector pixels. In this paper we present results of the SVD of each system and discuss calculation of the measurement and null space for each system.

  4. SPECT and PET in Eating Disorders

    NARCIS (Netherlands)

    van Waarde, Aren; Audenaert, Kurt; Busatto, Geraldo F.; Buchpiguel, Carlos; Dierckx, Rudi; Dierckx, Rudi AJO; Otte, Andreas; de Vries, Erik FJ; van Waarde, Aren; den Boer, Johan A

    2014-01-01

    Medical imaging techniques like PET and SPECT have been applied for investigation of brain function in anorexia and bulimia nervosa. Regional abnormalities have been detected in cerebral blood flow, glucose metabolism, the availability of several neurotransmitter receptors (serotonin 1A and 2A, dopa

  5. PET and SPECT imaging in veterinary medicine.

    Science.gov (United States)

    LeBlanc, Amy K; Peremans, Kathelijne

    2014-01-01

    Veterinarians have gained increasing access to positron emission tomography (PET and PET/CT) imaging facilities, allowing them to use this powerful molecular imaging technique for clinical and research applications. SPECT is currently being used more in Europe than in the United States and has been shown to be useful in veterinary oncology and in the evaluation of orthopedic diseases. SPECT brain perfusion and receptor imaging is used to investigate behavioral disorders in animals that have interesting similarities to human psychiatric disorders. This article provides an overview of the potential applications of PET and SPECT. The use of commercially available and investigational PET radiopharmaceuticals in the management of veterinary disease has been discussed. To date, most of the work in this field has utilized the commercially available PET tracer, (18)F-fluorodeoxyglucose for oncologic imaging. Normal biodistribution studies in several companion animal species (cats, dogs, and birds) have been published to assist in lesion detection and interpretation for veterinary radiologists and clinicians. Studies evaluating other (18)F-labeled tracers for research applications are underway at several institutions and companion animal models of human diseases are being increasingly recognized for their value in biomarker and therapy development. Although PET and SPECT technologies are in their infancy for clinical veterinary medicine, increasing access to and interest in these applications and other molecular imaging techniques has led to a greater knowledge and collective body of expertise for veterinarians worldwide. Initiation and fostering of physician-veterinarian collaborations are key components to the forward movement of this field.

  6. SPECT and PET in Eating Disorders

    NARCIS (Netherlands)

    van Waarde, Aren; Audenaert, Kurt; Busatto, Geraldo F.; Buchpiguel, Carlos; Dierckx, Rudi; Dierckx, Rudi AJO; Otte, Andreas; de Vries, Erik FJ; van Waarde, Aren; den Boer, Johan A

    2014-01-01

    Medical imaging techniques like PET and SPECT have been applied for investigation of brain function in anorexia and bulimia nervosa. Regional abnormalities have been detected in cerebral blood flow, glucose metabolism, the availability of several neurotransmitter receptors (serotonin 1A and 2A,

  7. SPECT using asymmetric pinholes with truncated projections

    Energy Technology Data Exchange (ETDEWEB)

    Lin Jianyu; Meikle, Steven R, E-mail: jianyu.lin@curtin.edu.au [Ramaciotti Imaging Centre, Brain and Mind Research Institute, University of Sydney (Australia)

    2011-07-07

    Tomographic systems employing truncated projections have been developed for parallel and fan beam collimation and for cone beam CT but the idea has not been extensively explored in pinhole single photon emission computed tomography (SPECT). In this paper, we explore the sampling requirements and system performance of SPECT systems with asymmetric pinhole collimators and truncated projections. We demonstrate that complete 3D sampling can be achieved by using multiple detectors with truncated asymmetric pinholes, offset axially from each other, and a spiral orbit. The use of truncated projections can be exploited in the design of pinhole SPECT systems by moving the pinholes closer to the subject, resulting in increased sensitivity and improved spatial resolution. Truncated and untruncated pinhole systems were evaluated using the contrast-to-noise ratio (CNR) calculated from the linearized local impulse response as a figure of merit. The CNR for the truncated pinhole system was up to 60% greater than that for the untruncated system at matched resolution for a source voxel near the centre of a uniform phantom and 30% greater at the edge. We conclude that an object can be reconstructed from asymmetric pinholes with truncated projections, which leads to potentially important design considerations and applications in single- and multi-pinhole SPECT.

  8. Differences in lateral hemispheric asymmetries of cerebral blood flow measured by SPECT in dementia of Alzheimer type

    Energy Technology Data Exchange (ETDEWEB)

    Yoshimura, Nahoko (Niigata Univ. (Japan). School of Medicine)

    1993-12-01

    We studied 21 right-handed patients clinically diagnosed as dementia of Alzheimer type (8 men, 13 women; aged 53-85, mean 71.1 years). The average duration of symptoms was 2.7 years. Dementia ranged from mild to moderately severe. None had clinical or laboratory evidence of cerebro-vascular disease (Hachinski ischemic scores for all patients were 4 or below). All received the Wechsler Adult Intelligence Scale (WAIS), Mini-mental State Test (MMS) and Western Aphasia Battery (WAB, First Japanese edition, 1986). Regional cerebral blood flow was evaluated by single photon emission CT (SPECT) with [sup 123]I-N-isopropyl-p-iodoamphetamine ([sup 123]I-IMP), using the Matsuda's quantitative method. The subjects were divided into three groups on the basis of right-left hemispheric asymmetry of cerebral blood flow (leftSPECT was related significantly to the deficit of language and constructive function in patients with dementia of Alzheimer type. (author).

  9. Cerebral perfusion SPECT in transient ischemic attack

    Energy Technology Data Exchange (ETDEWEB)

    You, D.-L. E-mail: dlyou@mail.kfcc.org.tw; Shieh, F.-Y.; Tzen, K.-Y.; Tsai, M.-F.; Kao, P.-F

    2000-04-01

    Purpose: The purpose of our study is to evaluate the efficacy of cerebral perfusion single photon emission computerized tomography (SPECT) in patients with transient ischemic attack (TIA). Methods: Thirty-seven patients with TIA were collected for study. All patients had transient focal neurological symptoms or signs with complete recovery within 24 h after onset. The patients underwent cerebral perfusion SPECT between 6 h and 11 days after onset, with 10 cases performed within 24 h (group A), nine cases performed between 1 and 3 days (group B), 11 cases performed between 3 and 5 days (group C), and seven cases performed after more than 5 days (group D). A semi-quantitative method was used for analyzing the SPECT data, and the difference ratios between lesion side and contralateral normal side were calculated on each pair of regions of interest. Results: In total, 78.4% (29/37) of patients had reduced perfusion in the cerebral cortical regions or deep nuclei, and the regions with reduced perfusion corresponded with clinical presentations of the patients. The abnormal rate with reduced perfusion was 90.0% in group A, 77.8% in group B, 72.7% in group C and 71.4% in group D. Cross cerebellar diaschisis (CCD) was present in seven patients, and all of the primary cerebral perfusion defects of these patients were located at the territory of left or right middle cerebral artery. Conclusion: Cerebral perfusion SPECT is a potential tool to detect cerebral perfusion defects and CCD in patients with TIA. Although the perfusion defect may persist more than 5 days after onset, we suggest cerebral perfusion SPECT should be performed as soon as possible.

  10. Brain pertechnetate SPECT in perinatal asphyxia

    Energy Technology Data Exchange (ETDEWEB)

    Sfakianakis, G.; Curless, R.; Goldberg, R.; Clarke, L.; Saw, C.; Sfakianakis, E.; Bloom, F.; Bauer, C.; Serafini, A.

    1984-01-01

    Single photon emission computed tomography of the brain was performed in 6 patients with perinatal asphyxis aged 8-26 days. A single-head (LFOV) commercial SPECT system (Picker) was used and data were acquired 2-3 hr after an IV injection of 1-2 mCi Tc-99m-pertechnetate (360/sup 0/ rotation, 60 views, 64 x 64 matrix, 50K cts/view). Reconstruction in three planes was performed using MDS software (Hanning medium resolution filter, with or without attenuation correction using Sorenson's technique). For each clinical study, a ring type phantom source was used to identify the level of reconstruction noise in the tomographic planes. Abnormalities were found in all patients studied, 3 central (moderate intensity), 2 peripheral (1 severe, 1 moderate) and 1 diffuse (mild intensity). Despite use of oral perchlorate (50 mg) in one patient the choroid plexus was visible. Since attenuation correction tended to amplify noise, the clinical studies were interpreted both with and without this correction. All 3 patients with central lesions were found abnormal on early (1-4 mo) neurologic follow-up examination, whereas the others were normal. No correlation was found between SPECT and 24 hr blood levels of CPK, ammonia, base excess, or the Apgar scores. Ct scans were reported abnormal (3 diffuse, 1 peripheral, 1 central and 1 questionable). Planar scintigrams obtained immediately after SPECT were normal (2), questionable (2) and abnormal (2). Follow-up SPECT brain scintigrams in two of the patients showed partial resolution. SPECT of the brain appears promising in perinatal asphyxia but long-term correlation with patient development is necessary.

  11. Monte Carlo scatter correction for SPECT

    Science.gov (United States)

    Liu, Zemei

    The goal of this dissertation is to present a quantitatively accurate and computationally fast scatter correction method that is robust and easily accessible for routine applications in SPECT imaging. A Monte Carlo based scatter estimation method is investigated and developed further. The Monte Carlo simulation program SIMIND (Simulating Medical Imaging Nuclear Detectors), was specifically developed to simulate clinical SPECT systems. The SIMIND scatter estimation (SSE) method was developed further using a multithreading technique to distribute the scatter estimation task across multiple threads running concurrently on multi-core CPU's to accelerate the scatter estimation process. An analytical collimator that ensures less noise was used during SSE. The research includes the addition to SIMIND of charge transport modeling in cadmium zinc telluride (CZT) detectors. Phenomena associated with radiation-induced charge transport including charge trapping, charge diffusion, charge sharing between neighboring detector pixels, as well as uncertainties in the detection process are addressed. Experimental measurements and simulation studies were designed for scintillation crystal based SPECT and CZT based SPECT systems to verify and evaluate the expanded SSE method. Jaszczak Deluxe and Anthropomorphic Torso Phantoms (Data Spectrum Corporation, Hillsborough, NC, USA) were used for experimental measurements and digital versions of the same phantoms employed during simulations to mimic experimental acquisitions. This study design enabled easy comparison of experimental and simulated data. The results have consistently shown that the SSE method performed similarly or better than the triple energy window (TEW) and effective scatter source estimation (ESSE) methods for experiments on all the clinical SPECT systems. The SSE method is proven to be a viable method for scatter estimation for routine clinical use.

  12. IMP2, a gene involved in the expression of glucose-repressible genes in Saccharomyces cerevisiae.

    Science.gov (United States)

    Lodi, T; Goffrini, P; Ferrero, I; Donnini, C

    1995-09-01

    Two mutants carrying different deletions of the IMP2 coding sequence of Saccharomyces cerevisiae, delta T1, which encodes a protein lacking the last 26 C-terminal amino acids, and delta T2, which completely lacks the coding region, were analysed for derepression of glucose-repressible maltose, galactose, raffinose and ethanol utilization pathways in response to glucose limitation. The role of the IMP2 gene product in the regulation of carbon catabolite repressible enzymes maltase, invertase, alcohol dehydrogenase, NAD-dependent glutamate dehydrogenase (NAD-GDH) and L-lactate:ferricytochrome-c oxidoreductase (L-LCR) was also analysed. The IMP2 gene product is required for the rapid glucose derepression of all above-mentioned carbon source utilization pathways and of all the enzymes except for L-LCR. NAD-GDH is regulated by IMP2 in the opposite way and, in fact, this enzyme was released at higher levels in both imp2 mutants than in the wild-type strain. Therefore, the product of IMP2 appears to be involved in positive and negative regulation. Both deletions result in growth and catalytic defects; in some cases partial modification of the gene product yielded more dramatic effects than its complete absence. Moreover, evidence is provided that the IMP2 gene product regulates galactose- and maltose-inducible genes at the transcriptional level and is a positive regulator of maltase, maltose permease and galactose permease gene expression.

  13. Isolation and plasmid characterization of carbapenemase (IMP-4) producing Salmonella enterica Typhimurium from cats

    Science.gov (United States)

    Abraham, Sam; O’Dea, Mark; Trott, Darren J.; Abraham, Rebecca J.; Hughes, David; Pang, Stanley; McKew, Genevieve; Cheong, Elaine Y. L.; Merlino, John; Saputra, Sugiyono; Malik, Richard; Gottlieb, Thomas

    2016-01-01

    Carbapenem-resistant Enterobacteriaceae (CRE) are a pressing public health issue due to limited therapeutic options to treat such infections. CREs have been predominantly isolated from humans and environmental samples and they are rarely reported among companion animals. In this study we report on the isolation and plasmid characterization of carbapenemase (IMP-4) producing Salmonella enterica Typhimurium from a companion animal. Carbapenemase-producing S. enterica Typhimurium carrying blaIMP-4 was identified from a systemically unwell (index) cat and three additional cats at an animal shelter. All isolates were identical and belonged to ST19. Genome sequencing revealed the acquisition of a multidrug-resistant IncHI2 plasmid (pIMP4-SEM1) that encoded resistance to nine antimicrobial classes including carbapenems and carried the blaIMP-4-qacG-aacA4-catB3 cassette array. The plasmid also encoded resistance to arsenic (MIC-150 mM). Comparative analysis revealed that the plasmid pIMP4-SEM1 showed greatest similarity to two blaIMP-8 carrying IncHI2 plasmids from Enterobacter spp. isolated from humans in China. This is the first report of CRE carrying a blaIMP-4 gene causing a clinical infection in a companion animal, with presumed nosocomial spread. This study illustrates the broader community risk entailed in escalating CRE transmission within a zoonotic species such as Salmonella, and in a cycle that encompasses humans, animals and the environment. PMID:27767038

  14. Prolonged lung retention of /sup 123/I-IMP in pulmonary disease

    Energy Technology Data Exchange (ETDEWEB)

    Ikeda, Hideki; Itasaka, Miyoko; Komatsu, Mikio; Takahashi, Keiji; Yasui, Shoji; Takahashi, Kazuei

    1989-10-01

    The accumulation of venously injected /sup 123/I-IMP in the lung was studied. At 30 and 50 min after the injection of the 1.5 mCi /sup 123/I-IMP, the concentration of /sup 123/I-IMP in the broncho-alveolar lavage fluid were much higher than in the blood. It was considered that /sup 123/I-IMP was transported into the alveolar spaces and was absorbed by the alveolar cells. The half time (T/sub 1/2/) of the /sup 123/I-IMP release from the lung at 10 and 25 min immediately after the injection was calculated. In normal subjects the T/sub 1/2/ ranged between 25 and 44 min and was prolonged in subjects with pulmonary fibrosis, sarcoidosis, and allergic alveolitis. It was considered that the retention of /sup 123/I-IMP was related not only to the endothelial cells, but also to the alveolar cells. It was considered that the analysis of the lung release of /sup 123/I-IMP forms a new lung dysfunction index. (orig.).

  15. Comparison and planar and SPECT ventilation/perfusion lung scanning

    Energy Technology Data Exchange (ETDEWEB)

    Eberl, S.; Uren, R.; Bautovich, G.; Dugal, T.; Forster, J. [Royal Prince Alfred Hospital, Camperdown, NSW (Australia). Department of PET and Nuclear Medicine

    1998-06-01

    Full text: Ventilation/perfusion (V/Q) lung scanning, using multiple planar views, is an accepted technique for diagnosing pulmonary embolism (PE). Under PIOPED criteria, there are a substantial number of indeterminate results due to confounding factors such as CAL, unusual anatomy or superimposed activity in the planar image. Our aim was to investigate if SPECT could improve the diagnostic accuracy of V/Q scanning in the diagnosis of PE. Both planar and SPECT images were acquired on a dual or a triple head gamma camera following inhalation of {sup 99m}Tc-Technegas and after injection of {sup 99m}Tc-MAA. Total acquisition time for SPECT studies was 7.5 min for ventilation and 5 min for perfusion. SPECT studies were reconstructed with an accelerated iterative reconstruction (OSEM) and ventilation slices and perfusion slices were registered with an automated image registration package. Total processing time was 5 min. We have now performed more than 600 planar and SPECT studies. A subset of 50 sequential patients and 10 cases with pulmonary angiography were scored blinded by experienced nuclear medicine physicians. Scores ranged from 0 (no PE) to 5 (definite PE). A score of 3 indicated indeterminate for PE. In the 60 patients analysed, planar and SPECT scores were equal in 27 patients, SPECT was more definite in 16 and less definite in 7 patients. In 8 patients, SPECT indicated PE and planar imaging low probability of PE. In 1 patient planar imaging was scored as PE and SPECT as low probability. Indeterminate rate was reduced from 8 to 1 with SPECT. In the 10 patients with angiography, SPECT studies agreed with angiography findings in 8 patients, while planar studies agreed with angiography findings in 5 patients. Our preliminary data suggest that SPECT V/Q lung scanning can: i) provide a significant improvement over planar imaging for the diagnosis of PE, and ii) our short acquisition time for V/Q SPECT is clinically practicable

  16. Emergence of Raoultella ornithinolytica coproducing IMP-4 and KPC-2 carbapenemases in China.

    Science.gov (United States)

    Zheng, Beiwen; Zhang, Jing; Ji, Jinru; Fang, Yunhui; Shen, Ping; Ying, Chaoqun; Lv, Jifang; Xiao, Yonghong; Li, Lanjuan

    2015-11-01

    We report here the emergence of seven IMP-4-producing Raoultella ornithinolytica isolates obtained from one patient. All isolates carried the blaIMP-4 carbapenemase gene, five isolates also carried blaSHV-12, four contained blaTEM-1, and one contained blaOXA-1. Notably, the R. ornithinolytica isolate Ro25724 also expressed Klebsiella pneumoniae carbapenemase (KPC)-2. The blaKPC-2 gene was located on a Tn3-Tn4401 integration structure on a plasmid of ∼450 kb. This is the first description of the coexistence of blaKPC-2 and blaIMP-4 from the genus Raoultella.

  17. Emergence of Raoultella ornithinolytica Coproducing IMP-4 and KPC-2 Carbapenemases in China

    Science.gov (United States)

    Zheng, Beiwen; Zhang, Jing; Ji, Jinru; Fang, Yunhui; Shen, Ping; Ying, Chaoqun; Lv, Jifang

    2015-01-01

    We report here the emergence of seven IMP-4-producing Raoultella ornithinolytica isolates obtained from one patient. All isolates carried the blaIMP-4 carbapenemase gene, five isolates also carried blaSHV-12, four contained blaTEM-1, and one contained blaOXA-1. Notably, the R. ornithinolytica isolate Ro25724 also expressed Klebsiella pneumoniae carbapenemase (KPC)-2. The blaKPC-2 gene was located on a Tn3-Tn4401 integration structure on a plasmid of ∼450 kb. This is the first description of the coexistence of blaKPC-2 and blaIMP-4 from the genus Raoultella. PMID:26282422

  18. Geometric calibration for a SPECT system dedicated to breast imaging

    Institute of Scientific and Technical Information of China (English)

    WU Li-Wei; WEI Long; CAO Xue-Xiang; WANG Lu; HUANG Xian-Chao; CHAI Pei; YUN Ming-Kai; ZHANG Yu-Bao; ZHANG Long; SHAN Bao-Ci

    2012-01-01

    Geometric calibration is critical to the accurate SPECT reconstruction.In this paper,a geometric calibration method was developed for a dedicated breast SPECT system with a tilted parallel beam (TPB)orbit.The acquisition geometry of the breast SPECT was firstly characterized.And then its projection model was established based on the acquisition geometry.Finally,the calibration results were obtained using a nonlinear optimization method that fitted the measured projections to the model.Monte Carlo data of the breast SPECT were used to verify the calibration method.Simulation results showed that the geometric parameters with reasonable accuracy could be obtained by the proposed method.

  19. Isolation of the first IMP-4 metallo-β-lactamase producing Klebsiella pneumoniae in Tianjin, China

    Directory of Open Access Journals (Sweden)

    Jing Li

    2012-09-01

    Full Text Available This study shows for the first time the mechanism of carbapenem resistance of a Klebsiella pneumoniae clinical isolate TJ8 recovered from Tianjin medical university general hospital ,China. The modified Hodge test and EDTA synergy test were performed for the screening of carbapenemases and metallo-β-lactamases (MBLs, respectively. Polymerase chain reactions and DNA sequencing confirmed that the strain carried IMP-4 metallo-β-lactamase (MBL , SHV-11 and TEM-1 β-lactamase. ClassⅠintegron was positive and gave a 3.0-kb PCR amplicon .IMP-4 was located in ClassⅠintegron 5'CS. The gene determinants were organized in the order of blaIMP-4-orfII-orfIII.In all, the results show that IMP-4 MBL production caused the TJ8 resistance to carbapenems.

  20. Isolation of the first IMP-4 metallo-β-lactamase producing Klebsiella pneumoniae in Tianjin, China

    Science.gov (United States)

    Li, Jing; Hu, Zhidong; Hu, Qiaojuan

    2012-01-01

    This study shows for the first time the mechanism of carbapenem resistance of a Klebsiella pneumoniae clinical isolate TJ8 recovered from Tianjin Medical University General Hospital ,China. The modified Hodge test and EDTA synergy test were performed for the screening of carbapenemases and metallo-β-lactamases (MBLs), respectively. Polymerase chain reactions and DNA sequencing confirmed that the strain carried IMP-4 metallo-β-lactamase (MBL) , SHV-11 and TEM-1 β-lactamase. Class I integron was positive and gave a 3.0-kb PCR amplicon .IMP-4 was located in Class I integron 5’CS. The gene determinants were organized in the order of blaIMP-4-orfII-orfIII.In all, the results show that IMP-4 MBL production caused the TJ8 resistance to carbapenems. PMID:24031907

  1. Drosophila Imp iCLIP identifies an RNA assemblage coordinating F-actin formation

    DEFF Research Database (Denmark)

    Hansen, Heidi Theil; Rasmussen, Simon Horskjær; Adolph, Sidsel Kramshøj;

    2015-01-01

    CLIP) technologies in Drosophila cells to identify transcripts associated with cytoplasmic ribonucleoproteins (RNPs) containing the RNA-binding protein Imp. RESULTS: We find extensive binding of Imp to 3'UTRs of transcripts that are involved in F-actin formation. A common denominator of the RNA-protein interface....... This demonstrates a physiological significance of the defined RNA regulon. CONCLUSIONS: Our data imply that Drosophila Imp RNPs may function as cytoplasmic mRNA assemblages that encode proteins which participate in actin cytoskeletal remodeling. Thus, they may facilitate co-ordinated protein expression in sub...... is the presence of multiple motifs with a central UA-rich element flanked by CA-rich elements. Experiments in single cells and intact flies reveal compromised actin cytoskeletal dynamics associated with low Imp levels. The former shows reduced F-actin formation and the latter exhibits abnormal neuronal patterning...

  2. SPECT Molecular Imaging in Parkinson's Disease

    Directory of Open Access Journals (Sweden)

    Ling Wang

    2012-01-01

    Full Text Available Parkinson's disease (PD is a common disorder, and the diagnosis of Parkinson's disease is clinical and relies on the presence of characteristic motor symptoms. The accuracy of the clinical diagnosis of PD is still limited. Functional neuroimaging using SPECT technique is helpful in patients with first signs of parkinsonism. The changes detected may reflect the disease process itself and/or compensatory responses to the disease, or they may arise in association with disease- and/or treatment-related complications. This paper addresses the value of SPECT in early differential diagnosis of PD and its potential as a sensitive tool to assess the pathophysiology and progression, as well as the therapeutic efficacy of PD.

  3. Methodology for ventilation/perfusion SPECT

    DEFF Research Database (Denmark)

    Bajc, Marika; Neilly, Brian; Miniati, Massimo;

    2010-01-01

    radiolabeled liquid aerosols are not restricted to the presence of obstructive lung disease. Radiolabeled macroaggregated human albumin is the imaging agent of choice for perfusion scintigraphy. An optimal combination of nuclide activities and acquisition times for ventilation and perfusion, collimators......Ventilation/perfusion single-photon emission computed tomography (V/Q SPECT) is the scintigraphic technique of choice for the diagnosis of pulmonary embolism and many other disorders that affect lung function. Data from recent ventilation studies show that the theoretic advantages of Technegas over......, and imaging matrix yields an adequate V/Q SPECT study in approximately 20 minutes of imaging time. The recommended protocol based on the patient remaining in an unchanged position during the initial ventilation study and the perfusion study allows presentation of matching ventilation and perfusion slices...

  4. Silicon Detectors for PET and SPECT

    Science.gov (United States)

    Cochran, Eric R.

    Silicon detectors use state-of-the-art electronics to take advantage of the semiconductor properties of silicon to produce very high resolution radiation detectors. These detectors have been a fundamental part of high energy, nuclear, and astroparticle physics experiments for decades, and they hold great potential for significant gains in both PET and SPECT applications. Two separate prototype nuclear medicine imaging systems have been developed to explore this potential. Both devices take advantage of the unique properties of high resolution pixelated silicon detectors, designed and developed as part of the CIMA collaboration and built at The Ohio State University. The first prototype is a Compton SPECT imaging system. Compton SPECT, also referred to as electronic collimation, is a fundamentally different approach to single photon imaging from standard gamma cameras. It removes the inherent coupling of spatial resolution and sensitivity in mechanically collimated systems and provides improved performance at higher energies. As a result, Compton SPECT creates opportunities for the development of new radiopharmaceuticals based on higher energy isotopes as well as opportunities to expand the use of current isotopes such as 131I due to the increased resolution and sensitivity. The Compton SPECT prototype consists of a single high resolution silicon detector, configured in a 2D geometry, in coincidence with a standard NaI scintillator detector. Images of point sources have been taken for 99mTc (140 keV), 131I (364keV), and 22Na (511 keV), demonstrating the performance of high resolution silicon detectors in a Compton SPECT system. Filtered back projection image resolutions of 10 mm, 7.5 mm, and 6.7 mm were achieved for the three different sources respectively. The results compare well with typical SPECT resolutions of 5-15 mm and validate the claims of improved performance in Compton SPECT imaging devices at higher source energies. They also support the potential of

  5. Diagnostic value of immunohistochemical IMP3 expression in core needle biopsies of pancreatic ductal adenocarcinoma.

    Science.gov (United States)

    Wachter, David Lukas; Schlabrakowski, Anne; Hoegel, Josef; Kristiansen, Glen; Hartmann, Arndt; Riener, Marc-Oliver

    2011-06-01

    The oncofetal protein, insulin-like growth factor II messenger ribonucleic acid-binding protein 3 (IMP3), has been analyzed in many different tumors. Various studies have found that IMP3 is a marker for malignancy and is correlated with increased tumor aggressiveness and reduced overall survival. The diagnosis of pancreatic ductal adenocarcinoma (PDAC) in core needle biopsies can be challenging, and immunohistochemical markers are needed. We studied IMP3 expression in 177 core needle biopsies of the pancreas, including 112 PDACs, 55 cases with chronic sclerosing pancreatitis, and 10 biopsies with tumor-free pancreatic tissue without inflammation. An additional 18 biopsies of PDAC metastases (16 liver biopsies and 2 lymph node biopsies) were analyzed. To study IMP3 expression in large tissue sections, 45 pancreatic resection specimens (26 with PDAC and 19 with chronic sclerosing pancreatitis) were investigated. In contrast to normal or inflamed pancreatic tissue, which was negative in 47 of 65 (72.3%) cases and weakly positive in 15 of 65 (23.1%) cases, strong IMP3 expression was found in 99 of 112 (88.4%) PDACs. Therefore, sensitivity and specificity of IMP3 expression in the differential diagnosis of PDAC and chronic sclerosing pancreatitis using core needle biopsies were found to be 88.4% and 94.6%, respectively. These results were confirmed in the pancreas resection specimens. Furthermore, strong IMP3 expression was found in 17 of 18 (94.4%) of the PDAC metastases that were analyzed. Our study shows that IMP3 is an easy to use and potentially new immunohistochemical marker for the diagnosis of PDAC in core needle biopsies.

  6. Dominance of IMP-4-Producing Enterobacter cloacae among Carbapenemase-Producing Enterobacteriaceae in Australia

    Science.gov (United States)

    Townell, Nicola; Nimmo, Graeme R.; George, Narelle M.; Robson, Jennifer; Vohra, Renu; Davis, Louise; Heney, Claire; Paterson, David L.

    2015-01-01

    The prevalence of carbapenemase-producing Enterobacteriaceae (CPE) has been increasing worldwide. blaIMP has been reported to be the predominant carbapenemase-encoding gene within Enterobacteriaceae in Australia. However, there are limited data currently available on CPE from Queensland, Australia. A total of 58 CPE isolates were isolated between July 2009 and March 2014 from Queensland hospitals. The clonality of isolates was determined by Diversilab repetitive sequence-based PCR. The isolates were investigated for the resistance mechanisms carbapenemase, extended-spectrum β-lactamase, and AmpC β-lactamase and for aminoglycoside resistance and plasmid-mediated quinolone resistance genes by PCR. The plasmid types associated with carbapenemase-encoding genes were characterized. The majority of the CPE were Enterobacter cloacae (n = 29). The majority of Queensland CPE isolates were IMP producers and comprised 11 species (n = 48). Nine NDM-producing Enterobacteriaceae were identified. One NDM-producing Klebsiella pneumoniae isolate coproduced OXA-48. One K. pneumoniae isolate was an OXA-181 producer. The incidence of IMP producers increased significantly in 2013. blaIMP-4 was found in all IMP-producing isolates. blaTEM, qnrB, and aacA4 were common among IMP-4 producers. The HI2 (67%) and L/M (21%) replicons were associated with blaIMP-4. All HI2 plasmids were of sequence type 1 (ST1). All but one of the NDM producers possessed blaCTX-M-15. The 16S rRNA methylase genes found among NDM producers were armA, rmtB, rmtC, and rmtF. The substantial increase in the prevalence of CPE in Queensland has been associated mainly with the emergence E. cloacae strains possessing HI2 plasmids carrying blaIMP-4 over the past 2 years. The importation of NDM producers and/or OXA-48-like producers in patients also contributed to the increased emergence of CPE. PMID:25918153

  7. {sup 99m}Tc-MIBI pinhole SPECT in primary hyperparathyroidism: comparison with conventional SPECT, planar scintigraphy and ultrasonography

    Energy Technology Data Exchange (ETDEWEB)

    Carlier, Thomas; Bodet-Milin, Caroline; Kraeber-Bodere, Francoise; Ansquer, Catherine [Hotel Dieu University Hospital, Nuclear Medicine Department, Nantes (France); INSERM CRCNA, Nantes (France); Oudoux, Aurore [Hotel Dieu University Hospital, Nuclear Medicine Department, Nantes (France); Mirallie, Eric [Hotel Dieu University Hospital, Surgery Department, Nantes (France); Seret, Alain [University of Liege, Institute of Physics, Experimental Medical Imaging, Liege (Belgium); Daumy, Isabelle [Ultrasonography Center, Nantes (France); Leux, Christophe [Saint Jacques University Hospital, PIMESP, Nantes (France)

    2008-03-15

    A pinhole collimator is routinely used to increase the resolution of scintigraphy. This prospective study was conducted to determine the interest of {sup 99m}Tc-MIBI pinhole single-photon emission computed tomography (SPECT) for the preoperative localisation of parathyroid lesions in primary hyperparathyroidism. All patients underwent a neck ultrasonography (US), {sup 99m}TcO{sub 4}{sup -} and {sup 99m}Tc-MIBI planar images and two consecutive SPECT with a parallel (C-SPECT) and a pinhole collimator (P-SPECT). P-SPECT was performed with a tilted detector equipped with a pinhole collimator and reconstructed with a dedicated OSEM algorithm. A diagnostic confidence score (CS) was assigned to each procedure considering intensity and extra-thyroidal location of suspected lesions: 0 = negative, 1 = doubtful, 2 = moderately positive, 3 = positive. The results of these preoperative localisation studies were compared with surgical, pathological and 6-month biological findings. Fifty-one patients cured after surgery were included. Surgery revealed 55 lesions (median weight 0.5 g, 11 in ectopy). Sensitivities of US, planar imaging, C-SPECT and P-SPECT were, respectively, 51, 76, 82 and 87%. Nine glands were only detected by tomography and five glands only by P-SPECT. {sup 99m}Tc-MIBI/{sup 99m}TcO{sub 4}{sup -} planar scans and P-SPECT were complementary and, when combined together, showed the highest sensitivity (93%). Compared with planar imaging and C-SPECT, P-SPECT increased CS for 42 and 53% of lesions, respectively, and contributed to markedly reduce the number of uncertain results. A combination of planar {sup 99m}Tc-MIBI/{sup 99m}TcO{sub 4}{sup -} scintigraphy and P-SPECT appears to be a highly accurate preoperative imaging procedure in primary hyperparathyroidism. (orig.)

  8. Accelerated GPU based SPECT Monte Carlo simulations

    Science.gov (United States)

    Garcia, Marie-Paule; Bert, Julien; Benoit, Didier; Bardiès, Manuel; Visvikis, Dimitris

    2016-06-01

    Monte Carlo (MC) modelling is widely used in the field of single photon emission computed tomography (SPECT) as it is a reliable technique to simulate very high quality scans. This technique provides very accurate modelling of the radiation transport and particle interactions in a heterogeneous medium. Various MC codes exist for nuclear medicine imaging simulations. Recently, new strategies exploiting the computing capabilities of graphical processing units (GPU) have been proposed. This work aims at evaluating the accuracy of such GPU implementation strategies in comparison to standard MC codes in the context of SPECT imaging. GATE was considered the reference MC toolkit and used to evaluate the performance of newly developed GPU Geant4-based Monte Carlo simulation (GGEMS) modules for SPECT imaging. Radioisotopes with different photon energies were used with these various CPU and GPU Geant4-based MC codes in order to assess the best strategy for each configuration. Three different isotopes were considered: 99m Tc, 111In and 131I, using a low energy high resolution (LEHR) collimator, a medium energy general purpose (MEGP) collimator and a high energy general purpose (HEGP) collimator respectively. Point source, uniform source, cylindrical phantom and anthropomorphic phantom acquisitions were simulated using a model of the GE infinia II 3/8" gamma camera. Both simulation platforms yielded a similar system sensitivity and image statistical quality for the various combinations. The overall acceleration factor between GATE and GGEMS platform derived from the same cylindrical phantom acquisition was between 18 and 27 for the different radioisotopes. Besides, a full MC simulation using an anthropomorphic phantom showed the full potential of the GGEMS platform, with a resulting acceleration factor up to 71. The good agreement with reference codes and the acceleration factors obtained support the use of GPU implementation strategies for improving computational efficiency

  9. Accelerated GPU based SPECT Monte Carlo simulations.

    Science.gov (United States)

    Garcia, Marie-Paule; Bert, Julien; Benoit, Didier; Bardiès, Manuel; Visvikis, Dimitris

    2016-06-07

    Monte Carlo (MC) modelling is widely used in the field of single photon emission computed tomography (SPECT) as it is a reliable technique to simulate very high quality scans. This technique provides very accurate modelling of the radiation transport and particle interactions in a heterogeneous medium. Various MC codes exist for nuclear medicine imaging simulations. Recently, new strategies exploiting the computing capabilities of graphical processing units (GPU) have been proposed. This work aims at evaluating the accuracy of such GPU implementation strategies in comparison to standard MC codes in the context of SPECT imaging. GATE was considered the reference MC toolkit and used to evaluate the performance of newly developed GPU Geant4-based Monte Carlo simulation (GGEMS) modules for SPECT imaging. Radioisotopes with different photon energies were used with these various CPU and GPU Geant4-based MC codes in order to assess the best strategy for each configuration. Three different isotopes were considered: (99m) Tc, (111)In and (131)I, using a low energy high resolution (LEHR) collimator, a medium energy general purpose (MEGP) collimator and a high energy general purpose (HEGP) collimator respectively. Point source, uniform source, cylindrical phantom and anthropomorphic phantom acquisitions were simulated using a model of the GE infinia II 3/8" gamma camera. Both simulation platforms yielded a similar system sensitivity and image statistical quality for the various combinations. The overall acceleration factor between GATE and GGEMS platform derived from the same cylindrical phantom acquisition was between 18 and 27 for the different radioisotopes. Besides, a full MC simulation using an anthropomorphic phantom showed the full potential of the GGEMS platform, with a resulting acceleration factor up to 71. The good agreement with reference codes and the acceleration factors obtained support the use of GPU implementation strategies for improving computational

  10. Staging of moyamoya disease by perfusion SPECT

    Energy Technology Data Exchange (ETDEWEB)

    Kuwabara, Yasuo [Kyushu Univ., Fukuoka (Japan). Hospital; Matsushima, Toshio; Fukui, Masashi

    2001-04-01

    Staging of moyamoya disease, based on angiography and PET have already been established. The authors have established staging of moyamoya disease based on perfusion SPECT, that can be summarized as follows: Stage I, no abnormality is seen at rest or after acetazolamide loading; Stage II, no abnormality is seen at rest, however, a decreased response (blood flow increase rate: <15%) is seen to acetazolamide loading (a, a decreased response is seen only in the frontal lobe; b, a decreased response is seen in regions other than the frontal lobe; and c, a decreased response is seen throughout the cerebrum); Stage III, localized decrease in blood flow (blood flow decrease compared with peripheral tissue: {>=}15%) and marked decrease in response to acetazolamide (blood flow increase rate: <5%) are seen at rest. In Stage III, CT and MRI show no abnormal findings or only mild lesions of the white matter; and Stage IV, multiple decreases in blood flow are seen at rest, and CT and MRI reveal infarctions and severe atrophy at the same sites. The above staging does not require determination of cerebral blood flow, and thus it can be used in children, in whom cerebral blood flow determination is difficult. The authors performed 99m-Tc ECD perfusion SPECT in 25 patients with moyamoya disease for the staging, and compared staging based on angiography with staging based on perfusion SPECT. The results did not show a correlation between the 2 staging methods. A problem inherent in the staging of moyamoya disease based on perfusion SPECT is that the relationship between cerebral blood flow and cerebral radioactivity concentrations may differ depending on the drug used to determine cerebral blood flow. Thus, although the present staging system does not depend on any specific radioactive drug to determine cerebral blood flow, further investigation is necessary to identify a more appropriate drug than those in current use. (K.H.)

  11. ENVISION, developing SPECT imaging for particle therapy

    CERN Multimedia

    2013-01-01

    Particle therapy is an advanced technique of cancer radiation therapy, using protons or other ions to target the cancerous mass. ENVISION aims at developing medical imaging tools to improve the dose delivery to the patient, to ensure a safer and more effective treatment. The animation illustrates the use of Single Photon Emission Computed Tomography (SPECT) for monitoring the dose during treatment. Produced by: CERN KT/Life Sciences and ENVISION Project Management: Manuela Cirilli 3D animation: Jeroen Huijben, Nymus3d

  12. Compartment analysis of {sup 123}I-iomazenil brain SPECT in patients with moyamoya disease

    Energy Technology Data Exchange (ETDEWEB)

    Kaneta, Tomohiro; Yamazaki, Tetsuro; Takahashi, Shoki; Yamada, Shogo [Tohoku Univ., Sendai (Japan). School of Medicine; Maruoka, Shin; Abe, Yoetsu

    1999-12-01

    We investigated 11 patients with moyamoya disease about {sup 123}I-Iomazenil kinetics in the brain using three-compartment, two-parameter model. The transition rate constant (K1) from the blood to the brain and the binding potential (BP) of the benzodiazepine to the receptors were calculated for every ROI (right and left side of cerebellum, frontal lobe, parietal lobe, occipital lobe and temporal lobe; 10 ROIs a case). The K1 value correlated with BP value significantly, but not so closely (r=0.639). And there is no significant difference in BP valued among low-K1 group (mean (of K1)-S.D.{<=}K1{<=}mean) and high-K1 group (meanIMP/IMZ discrepancy. The nerve cell in the hypoperfused area which has almost normal BP value is ischemic but viable. IMZ-SPECT presents an important information about the viability of the hypoperfused area in moyamoya disease patients' brain. (author)

  13. SPECT and PET Imaging of Meningiomas

    Directory of Open Access Journals (Sweden)

    Varvara Valotassiou

    2012-01-01

    Full Text Available Meningiomas arise from the meningothelial cells of the arachnoid membranes. They are the most common primary intracranial neoplasms and represent about 20% of all intracranial tumors. They are usually diagnosed after the third decade of life and they are more frequent in women than in men. According to the World Health Organization (WHO criteria, meningiomas can be classified into grade I meningiomas, which are benign, grade II (atypical and grade III (anaplastic meningiomas, which have a much more aggressive clinical behaviour. Computed Tomography (CT and Magnetic Resonance Imaging (MRI are routinely used in the diagnostic workup of patients with meningiomas. Molecular Nuclear Medicine Imaging with Single Photon Emission Computed Tomography (SPECT and Positron Emission Tomography (PET could provide complementary information to CT and MRI. Various SPECT and PET tracers may provide information about cellular processes and biological characteristics of meningiomas. Therefore, SPECT and PET imaging could be used for the preoperative noninvasive diagnosis and differential diagnosis of meningiomas, prediction of tumor grade and tumor recurrence, response to treatment, target volume delineation for radiation therapy planning, and distinction between residual or recurrent tumour from scar tissue.

  14. SPECT detectors: the Anger Camera and beyond

    Science.gov (United States)

    Peterson, Todd E.; Furenlid, Lars R.

    2011-09-01

    The development of radiation detectors capable of delivering spatial information about gamma-ray interactions was one of the key enabling technologies for nuclear medicine imaging and, eventually, single-photon emission computed tomography (SPECT). The continuous sodium iodide scintillator crystal coupled to an array of photomultiplier tubes, almost universally referred to as the Anger Camera after its inventor, has long been the dominant SPECT detector system. Nevertheless, many alternative materials and configurations have been investigated over the years. Technological advances as well as the emerging importance of specialized applications, such as cardiac and preclinical imaging, have spurred innovation such that alternatives to the Anger Camera are now part of commercial imaging systems. Increased computing power has made it practical to apply advanced signal processing and estimation schemes to make better use of the information contained in the detector signals. In this review we discuss the key performance properties of SPECT detectors and survey developments in both scintillator and semiconductor detectors and their readouts with an eye toward some of the practical issues at least in part responsible for the continuing prevalence of the Anger Camera in the clinic.

  15. SPECT detectors: the Anger Camera and beyond.

    Science.gov (United States)

    Peterson, Todd E; Furenlid, Lars R

    2011-09-07

    The development of radiation detectors capable of delivering spatial information about gamma-ray interactions was one of the key enabling technologies for nuclear medicine imaging and, eventually, single-photon emission computed tomography (SPECT). The continuous sodium iodide scintillator crystal coupled to an array of photomultiplier tubes, almost universally referred to as the Anger Camera after its inventor, has long been the dominant SPECT detector system. Nevertheless, many alternative materials and configurations have been investigated over the years. Technological advances as well as the emerging importance of specialized applications, such as cardiac and preclinical imaging, have spurred innovation such that alternatives to the Anger Camera are now part of commercial imaging systems. Increased computing power has made it practical to apply advanced signal processing and estimation schemes to make better use of the information contained in the detector signals. In this review we discuss the key performance properties of SPECT detectors and survey developments in both scintillator and semiconductor detectors and their readouts with an eye toward some of the practical issues at least in part responsible for the continuing prevalence of the Anger Camera in the clinic.

  16. Expression of IMP3 protein in cervical carcinoma and the significance of its relationship with MVD

    Institute of Scientific and Technical Information of China (English)

    Ming Li; Ya-Jun Zhang

    2015-01-01

    Objective:To study the expressions of IMP3 and MVD in normal cervical tissue, CIN, and squamous cell carcinoma of cervix and investigate their relationship with the occurrence and development of cervical carcinoma.Methods:Immunohistochemistry S-P method was used to detect the expressions of IMP3 and MVD in 50 cervical invasive carcinoma tissues, 35 CIN tissues and 15 cervicitis tissues respectively.Results: There were significant differences in pair wise comparison of the expressions of IMP3 and MVD in cervical carcinoma, CIN and normal cervical tissues (P<0.05). IMP3 and MVD value were highly expressed in cervical carcinoma tissues, and were correlated with clinical staging, lymphatic metastasis and differentiation degree of cervical carcinoma (P<0.05); IMP3 and MVD value were correlated with their expressions in cervical carcinoma tissues (r=0.323P=0.022).Conclusion: IMP3 and MVD are associated with the occurrence as well as the invasion and metastasis of cervical carcinoma, and can be used as a proper indicator of the malignancy degree and prognosis of cervical carcinoma.

  17. Performance evaluation of D-SPECT: a novel SPECT system for nuclear cardiology

    Energy Technology Data Exchange (ETDEWEB)

    Erlandsson, Kjell; Kacperski, Krzysztof; Van Gramberg, Dean; Hutton, Brian F [Institute of Nuclear Medicine, University College London and UCLH NHS Foundation Trust, London NW1 2BU (United Kingdom)], E-mail: kjell.erlandsson@uclh.nhs.uk

    2009-05-07

    D-SPECT (Spectrum Dynamics, Israel) is a novel SPECT system for cardiac perfusion studies. Based on CZT detectors, region-centric scanning, high-sensitivity collimators and resolution recovery, it offers potential advantages over conventional systems. A series of measurements were made on a {beta}-version D-SPECT system in order to evaluate its performance in terms of energy resolution, scatter fraction, sensitivity, count rate capability and resolution. Corresponding measurements were also done on a conventional SPECT system (CS) for comparison. The energy resolution of the D-SPECT system at 140 keV was 5.5% (CS: 9.25%), the scatter fraction 30% (CS: 34%), the planar sensitivity 398 s{sup -1} MBq{sup -1} per head ({sup 99m}Tc, 10 cm) (CS: 72 s{sup -1} MBq{sup -1}), and the tomographic sensitivity in the heart region was in the range 647-1107 s{sup -1} MBq{sup -1} (CS: 141 s{sup -1} MBq{sup -1}). The count rate increased linearly with increasing activity up to 1.44 M s{sup -1}. The intrinsic resolution was equal to the pixel size, 2.46 mm (CS: 3.8 mm). The average reconstructed resolution using the standard clinical filter was 12.5 mm (CS: 13.7 mm). The D-SPECT has superior sensitivity to that of a conventional system with similar spatial resolution. It also has excellent energy resolution and count rate characteristics, which should prove useful in dynamic and dual radionuclide studies.

  18. Medida Interpessoal de Psicopatia (IM-P: estudo preliminar no contexto brasileiro Interpersonal Measure of Psychopathy (IM-P: preliminary study in the Brazilian context

    Directory of Open Access Journals (Sweden)

    Tárcia Rita Davoglio

    2011-01-01

    Full Text Available INTRODUÇÃO: A observação direta do comportamento interpessoal é um recurso importante na descrição e diagnóstico da personalidade psicopática. A Medida Interpessoal de Psicopatia (Interpersonal Measure of Psychopathy, IM-P é um instrumento psicométrico composto por 21 itens, desenvolvido para ser utilizado em associação com outras escalas de avaliação da psicopatia. Foca-se, especificamente, nos comportamentos interpessoais e aspectos não verbais evidentes na interação do entrevistador com indivíduos que apresentam características psicopáticas. OBJETIVO: Descrever resultados preliminares sobre a investigação de aspectos interpessoais da psicopatia mediante a utilização da IM-P, incluindo as etapas de tradução/adaptação e avaliação de confiabilidade interavaliadores da IM-P, em uma amostra de adolescentes brasileiros. MÉTODO: Trata-se de estudo transversal, descritivo e correlacional realizado com 20 adolescentes masculinos cumprindo medida socioeducativa com privação de liberdade na Região Metropolitana de Porto Alegre (RS. Após os procedimentos de tradução da escala, treinamento dos pesquisadores e teste piloto por meio de uma entrevista semiestruturada, a IM-P foi pontuada por três juízes independentes. RESULTADOS: Os resultados estatísticos, obtidos através do coeficiente de concordância de Kendall, revelaram grau de concordância interavaliadores elevado e satisfatório para os escores totais da IM-P (W = 0,84; p INTRODUCTION: The direct observation of interpersonal behaviors is an important resource in the description and diagnosis of the psychopathic personality. The Interpersonal Measure of Psychopathy (IM-P is a psychometric instrument comprised of 21 items, designed to be applied in association with other instruments that also evaluate psychopaths. It focuses specifically on interpersonal and non-verbal behaviors that become evident during the interaction between the interviewer and individuals

  19. SPECT and PET imaging in epilepsia; SPECT und PET in der Diagnostik von Epilepsien

    Energy Technology Data Exchange (ETDEWEB)

    Landvogt, C. [Mainz Univ. (Germany). Klinik und Poliklinik fuer Nuklearmedizin

    2007-09-15

    In preoperative localisation of epileptogenic foci, nuclear medicine diagnostics plays a crucial role. FDG-PET is used as first line diagnostics. In case of inconsistent MRI, EEG and FDG-PET findings, {sup 11}C-Flumazenil-PET or ictal and interictal perfusion-SPECT should be performed. Other than FDG, Flumazenil can help to identify the extend of the region, which should be resected. To enhance sensitivity and specificity, further data analysis using voxelbased statistical analyses or SISCOM (substraction ictal SPECT coregistered MRI) should be performed.

  20. A multiresolution restoration method for cardiac SPECT

    Science.gov (United States)

    Franquiz, Juan Manuel

    Single-photon emission computed tomography (SPECT) is affected by photon attenuation and image blurring due to Compton scatter and geometric detector response. Attenuation correction is important to increase diagnostic accuracy of cardiac SPECT. However, in attenuation-corrected scans, scattered photons from radioactivity in the liver could produce a spillover of counts into the inferior myocardial wall. In the clinical setting, blurring effects could be compensated by restoration with Wiener and Metz filters. Inconveniences of these procedures are that the Wiener filter depends upon the power spectra of the object image and noise, which are unknown, while Metz parameters have to be optimized by trial and error. This research develops an alternative restoration procedure based on a multiresolution denoising and regularization algorithm. It was hypothesized that this representation leads to a more straightforward and automatic restoration than conventional filters. The main objective of the research was the development and assessment of the multiresolution algorithm for compensating the liver spillover artifact. The multiresolution algorithm decomposes original SPECT projections into a set of sub-band frequency images. This allows a simple denoising and regularization procedure by discarding high frequency channels and performing inversion only in low and intermediate frequencies. The method was assessed in bull's eye polar maps and short- axis attenuation-corrected reconstructions of a realistic cardiac-chest phantom with a custom-made liver insert and different 99mTc liver-to-heart activity ratios. Inferior myocardial defects were simulated in some experiments. The cardiac phantom in free air was considered as the gold standard reference. Quantitative analysis was performed by calculating contrast of short- axis slices and the normalized chi-square measure, defect size and mean and standard deviation of polar map counts. The performance of the multiresolution

  1. Quantitative Monte Carlo-based holmium-166 SPECT reconstruction

    Energy Technology Data Exchange (ETDEWEB)

    Elschot, Mattijs; Smits, Maarten L. J.; Nijsen, Johannes F. W.; Lam, Marnix G. E. H.; Zonnenberg, Bernard A.; Bosch, Maurice A. A. J. van den; Jong, Hugo W. A. M. de [Department of Radiology and Nuclear Medicine, University Medical Center Utrecht, Heidelberglaan 100, 3584 CX Utrecht (Netherlands); Viergever, Max A. [Image Sciences Institute, University Medical Center Utrecht, Heidelberglaan 100, 3584 CX Utrecht (Netherlands)

    2013-11-15

    Purpose: Quantitative imaging of the radionuclide distribution is of increasing interest for microsphere radioembolization (RE) of liver malignancies, to aid treatment planning and dosimetry. For this purpose, holmium-166 ({sup 166}Ho) microspheres have been developed, which can be visualized with a gamma camera. The objective of this work is to develop and evaluate a new reconstruction method for quantitative {sup 166}Ho SPECT, including Monte Carlo-based modeling of photon contributions from the full energy spectrum.Methods: A fast Monte Carlo (MC) simulator was developed for simulation of {sup 166}Ho projection images and incorporated in a statistical reconstruction algorithm (SPECT-fMC). Photon scatter and attenuation for all photons sampled from the full {sup 166}Ho energy spectrum were modeled during reconstruction by Monte Carlo simulations. The energy- and distance-dependent collimator-detector response was modeled using precalculated convolution kernels. Phantom experiments were performed to quantitatively evaluate image contrast, image noise, count errors, and activity recovery coefficients (ARCs) of SPECT-fMC in comparison with those of an energy window-based method for correction of down-scattered high-energy photons (SPECT-DSW) and a previously presented hybrid method that combines MC simulation of photopeak scatter with energy window-based estimation of down-scattered high-energy contributions (SPECT-ppMC+DSW). Additionally, the impact of SPECT-fMC on whole-body recovered activities (A{sup est}) and estimated radiation absorbed doses was evaluated using clinical SPECT data of six {sup 166}Ho RE patients.Results: At the same noise level, SPECT-fMC images showed substantially higher contrast than SPECT-DSW and SPECT-ppMC+DSW in spheres ≥17 mm in diameter. The count error was reduced from 29% (SPECT-DSW) and 25% (SPECT-ppMC+DSW) to 12% (SPECT-fMC). ARCs in five spherical volumes of 1.96–106.21 ml were improved from 32%–63% (SPECT-DSW) and 50%–80

  2. Development and evaluation of Monte Carlo-based SPECT reconstruction

    NARCIS (Netherlands)

    Xiao, J.

    2009-01-01

    Single Photon Emission Computed Tomography (SPECT) is one of the most applied molecular imaging techniques to diagnose human diseases, e.g., of the heart, the brain or in oncology. For example, cardiac SPECT imaging plays a central role in diagnosing coronary heart diseases by providing clinicians w

  3. Ultra-high-resolution small-animal SPECT imaging

    NARCIS (Netherlands)

    Have, F. van der

    2007-01-01

    The main subject of this thesis is the development of the first two in a series of dedicated ultra-high resolution Single Photon Emission Computed Tomography (SPECT) systems (U-SPECT-I and II) for the imaging of distributions of radio-isotope labeled tracers in small laboratory animals such as mice

  4. Simulation, construction and application of focused pinhole small animal SPECT

    NARCIS (Netherlands)

    Vastenhouw, B.

    2008-01-01

    New developments in molecular imaging techniques like small animal SPECT systems are important tools to analyze mouse models of human diseases. The main subjects of this thesis are simulation, construction and image reconstruction algorithms needed for the development of a small-animal SPECT system

  5. Gelman: El salario del impío

    Directory of Open Access Journals (Sweden)

    Jorge José Monteleone

    2001-11-01

    Full Text Available En su "Discurso sobre lírica y sociedad", Theodor W Adorno señala que el poema lírico debe presentar un alto "grado de interiorización y una radical individuación". Es decir, que el contenido del poema será tanto más objetivo y motivado socialmente cuanto menos se "tematice" en el la relación entre el yo y la sociedad y que el sujeto estará menos ausente de la forma poética que lo presenta, cuanto mas se sumerja y olvide en la objetividad social del lenguaje. A k luz de esas nociones pueden leerse dos libros de Juan Gelman, que se refieren explícitamente al exilio: Bajo la lluvia ajena (notas al pie de una derrota (1984 y Salarios del impío (1993. Entre uno y otro se percibe una progresiva individuación que permite alcanzar formalmente en el lenguaje poético una eficaz representación imaginaria del hecho histórico opresivo, que elude la reducción temática. Asimismo, la noción de posterioridad, con k cual se reinscribe y reorganiza la significación de un hecho traumático del pasado en un trabajo de la memoria, permitiría revisar aquella célebre advertencia de Adorno según la cual seria imposible escribir poesía después de un genocidio.

  6. {sup 123}I-IMP SPET in the diagnosis of primary central nervous system lymphoma

    Energy Technology Data Exchange (ETDEWEB)

    Yoshikai, Tomonori; Fukahori, Tetsuhiro; Ishimaru, Junichiro; Kato, Akira; Uchino, Akira; Kudo, Sho [Dept. of Radiology, Saga Medical School, Nabeshima (Japan); Tabuchi, Kazuo [Dept. of Neurosurgery, Saga Medical School (Japan)

    2001-01-01

    We reviewed N-isopropyl-p-[{sup 123}I]iodoamphetamine ({sup 123}I-IMP) single-photon emission tomography (SPET) images of brain tumours and assessed the usefulness of {sup 123}I-IMP SPET for the diagnosis of primary central nervous system (CNS) lymphoma. We analysed 52 tumours that showed enhancement on magnetic resonance imaging: 11 malignant lymphomas, 3 anaplastic astrocytomas, 17 glioblastomas, 12 meningiomas, 4 metastatic brain tumours and 5 other brain tumours. {sup 123}I-IMP uptake in the tumours on early (15-min) and delayed (4-h) scans was visually classified as high, moderate or low as compared with the contralateral brain cortex. Early and delayed {sup 123}I-IMP uptake ratios comparing tumours with contralateral brain cortex (T/N ratio) were also calculated. In malignant lymphomas, the visual uptake of {sup 123}I-IMP was moderate to high on the delayed scans. The delayed T/N ratios were significantly higher than the early ratios (P<0.05) and all lymphomas, with the exception of one small one, had delayed ratios greater than 0.9. In non-lymphomatous tumours, the visual uptake of {sup 123}I-IMP was low on the delayed scans. The delayed T/N ratios were significantly lower than the early ratios (P<0.01) and all non-lymphomatous tumours had delayed ratios of less than 0.8. The T/N ratios of lymphomas were significantly higher than those of non-lymphomatous tumours on both early and delayed scans (P<0.0001). These results suggest that {sup 123}I-IMP SPET may be a useful tool in the differential diagnosis of primary CNS lymphoma. (orig.)

  7. Single photon emission computed tomography (SPECT) in epilepsy

    Energy Technology Data Exchange (ETDEWEB)

    Leroy, R.F. [Univ. of Texas Southwestern Medical School, Dallas (United States)

    1991-12-31

    Epilepsy is a common neurologic disorder which has just begun to be studied with single photon emission computerized tomography (SPECT). Epilepsy usually is studied with electroencephalographic (EEG) techniques that demonstrate the physiologic changes that occur during seizures, and with neuroimaging techniques that show the brain structures where seizures originate. Neither method alone has been adequate to describe the pathophysiology of the patient with epilepsy. EEG techniques lack anatomic sensitivity, and there are no structural abnormalities shown by neuroimaging which are specific for epilepsy. Functional imaging (FI) has developed as a physiologic tool with anatomic sensitivity, and SPECT has been promoted as a FI technique because of its potentially wide availability. However, SPECT is early in its development and its clinical utility for epilepsy still has to be demonstrated. To understand this role of SPECT, consideration must be given to the pathophysiology of epilepsy, brain physiology, types of seizure, epileptic syndromes, and the SPECT technique itself. 44 refs., 2 tabs.

  8. Performance of a high-sensitivity dedicated cardiac SPECT scanner for striatal uptake quantification in the brain based on analysis of projection data

    Energy Technology Data Exchange (ETDEWEB)

    Park, Mi-Ae; Moore, Stephen C.; McQuaid, Sarah J.; Kijewski, Marie Foley [Department of Radiology, Brigham and Women' s Hospital and Harvard Medical School, Boston, Massachusetts 02115 (United States); Mueller, Stefan P. [Abteilung Nuklearmedizin, Universitaetsklinikum Essen, 45147 Essen (Germany)

    2013-04-15

    Purpose: The authors have previously reported the advantages of high-sensitivity single-photon emission computed tomography (SPECT) systems for imaging structures located deep inside the brain. DaTscan (Isoflupane I-123) is a dopamine transporter (DaT) imaging agent that has shown potential for early detection of Parkinson disease (PD), as well as for monitoring progression of the disease. Realizing the full potential of DaTscan requires efficient estimation of striatal uptake from SPECT images. They have evaluated two SPECT systems, a conventional dual-head gamma camera with low-energy high-resolution collimators (conventional) and a dedicated high-sensitivity multidetector cardiac imaging system (dedicated) for imaging tasks related to PD. Methods: Cramer-Rao bounds (CRB) on precision of estimates of striatal and background activity concentrations were calculated from high-count, separate acquisitions of the compartments (right striata, left striata, background) of a striatal phantom. CRB on striatal and background activity concentration were calculated from essentially noise-free projection datasets, synthesized by scaling and summing the compartment projection datasets, for a range of total detected counts. They also calculated variances of estimates of specific-to-nonspecific binding ratios (BR) and asymmetry indices from these values using propagation of error analysis, as well as the precision of measuring changes in BR on the order of the average annual decline in early PD. Results: Under typical clinical conditions, the conventional camera detected 2 M counts while the dedicated camera detected 12 M counts. Assuming a normal BR of 5, the standard deviation of BR estimates was 0.042 and 0.021 for the conventional and dedicated system, respectively. For an 8% decrease to BR = 4.6, the signal-to-noise ratio were 6.8 (conventional) and 13.3 (dedicated); for a 5% decrease, they were 4.2 (conventional) and 8.3 (dedicated). Conclusions: This implies that PD can

  9. Bayesian learning for cardiac SPECT image interpretation.

    Science.gov (United States)

    Sacha, Jarosław P; Goodenday, Lucy S; Cios, Krzysztof J

    2002-01-01

    In this paper, we describe a system for automating the diagnosis of myocardial perfusion from single-photon emission computerized tomography (SPECT) images of male and female hearts. Initially we had several thousand of SPECT images, other clinical data and physician-interpreter's descriptions of the images. The images were divided into segments based on the Yale system. Each segment was described by the physician as showing one of the following conditions: normal perfusion, reversible perfusion defect, partially reversible perfusion defect, fixed perfusion defect, defect showing reverse redistribution, equivocal defect or artifact. The physician's diagnosis of overall left ventricular (LV) perfusion, based on the above descriptions, categorizes a study as showing one or more of eight possible conditions: normal, ischemia, infarct and ischemia, infarct, reverse redistribution, equivocal, artifact or LV dysfunction. Because of the complexity of the task, we decided to use the knowledge discovery approach, consisting of these steps: problem understanding, data understanding, data preparation, data mining, evaluating the discovered knowledge and its implementation. After going through the data preparation step, in which we constructed normal gender-specific models of the LV and image registration, we ended up with 728 patients for whom we had both SPECT images and corresponding diagnoses. Another major contribution of the paper is the data mining step, in which we used several new Bayesian learning classification methods. The approach we have taken, namely the six-step knowledge discovery process has proven to be very successful in this complex data mining task and as such the process can be extended to other medical data mining projects.

  10. Preliminary study in patients with vertigo and dizziness using /sup 123/I-IMP

    Energy Technology Data Exchange (ETDEWEB)

    Tamamoto, Fumihiko; Sakamoto, Naotaka; Shindo, Noboru; Hirano, Akira; Amemiya, Ken; Sumi, Yukiharu; Katayama, Hitoshi

    1989-01-01

    To study the application of cerebral blood flow scintigraphy with /sup 123/I-IMP for the evaluation of central dizziness, the difference in /sup 123/I-IMP accumulation between the right and left cerebellar hemispheres was determined as an asymmetry index (A.I.) in 8 normal subjects and compared with A.I. from patients with central dizziness. According to estimated lesions, /sup 123/I-IMP findings were compared with CT findings. The normal subjects had A.I. of 5.8+-2.1%, whereas 36 patients with central dizziness had A.I. of 12.7+-7.5%. On the basis of an A.I. of 8% or more as an abnormal finding on IMP scintigraphy, 11 of the 14 patients with estimated lesions of cerebellar hemispheres and 16 of the 20 with those of the whole cerebellum including the vermis were evaluated to have had abnormalities. Seven of 11 with estimated lesions of the brainstem and 4 of 5 with vertebrobasilar arterial insufficiency were evaluated to have had abnormalities. The findings of /sup 123/I-IMP cerebral blood flow scintigraphy were in good agreement with clinical symptoms in patients with central dizziness with estimated lesions of the cerebellar hemispheres, suggesting the possibility that this method is more useful than CT.

  11. Impératifs et limites de la croissance

    Directory of Open Access Journals (Sweden)

    Rolf Steppacher

    2006-07-01

    Full Text Available La théorie de la croissance domine l’actualité. Pourtant, il devient chaque jour plus évident que la poursuite de ce programme mondial n’est pas indéfiniment réalisable, compte tenu des limites imposées par les écosystèmes. Plus surprenant encore, l’impératif de croissance est parvenu à se dissimuler sous le masque de la durabilité. Devenu « durable » ou « soutenable », le développement s’accommode plutôt bien du capitalisme, sans questionner pour autant le cœur du paradoxe contemporain : comment parler de durabilité sans remettre en cause en même temps le système économique ? Comment maintenir une croissance illimitée – elle-même alimentée par une industrie publicitaire créatrice de désirs illimités – sans remettre parallèlement en question l’utilisation que nous faisons des ressources non renouvelables ? Plus que jamais et à l’heure où le développement durable s’impose comme le nouveau messianisme technologique, il devient vital de s’interroger sur le fonctionnement nécessairement problématique des économies modernes et de leurs choix énergétiques. Aller au-delà du consensus politique actuel fondé sur la tyrannie de la croissance, ne pas succomber à l’incantation technocratique, penser l’action, l’innovation et les transformations économiques génératrices du bien-être, concevoir que toute société porte en elle un projet qui ne se réduit pas à une capacité gestionnaire, rechercher les sources de la richesse et les raisons de sa fin possible. Voilà quelques-unes des ambitions de Rolf Steppacher, économiste à l’institut universitaire d’études du développement (IUED à Genève et observateur critique de l’évolution du développement durable et de son pendant, la décroissance. Il nous livre ici quelques clés d’interprétation. Entretien réalisé par Olivier Walther.

  12. Evaluation of Tl-201 SPECT imaging findings in prostate cancer

    Directory of Open Access Journals (Sweden)

    Sinem Ozyurt

    2015-07-01

    Full Text Available Objectives: To compare with histopathological findings the findings of prostate cancer imaging by SPECT method using Tl-201 as a tumor seeking agent. Methods: The study comprised 59 patients (age range 51-79 years, mean age 65.3 ± 6.8 years who were planned to have transrectal ultrasonography (TRUS-guided biopsies due to suspicion of prostate cancer between April 2011 and September 2011. Early planar, late planar and SPECT images were obtained for all patients. Scintigraphic evaluation was made in relation to uptake presence and patterns in the visual assessment and to Tumor/Background (T/Bg ratios for both planar and SPECT images in the quantitative assessment. Histopathological findings were compatible with benign etiology in 36 (61% patients and malign etiology in 23 (39% patients. Additionally, comparisons were made to evaluate the relationships between uptake patterns,total PSA values and Gleason scores. Results: A statistically significant difference was found between the benign and malignant groups in terms of uptake in planar and SPECT images and T/Bg ratios and PSA values. No statistically significant difference was found between uptake patterns of planar and SPECT images and Gleason scores in the malignant group. Conclusions: SPECT images were superior to planar images in the comparative assessment. Tl-201 SPECT imaging can provide an additional contribution to clinical practice in the diagnosis of prostate cancer and it can be used in selected patients.

  13. SPECT Imaging of patients with parkinsonian syndromes; SPECT-Untersuchungen bei Patienten mit Parkinson-Syndromen

    Energy Technology Data Exchange (ETDEWEB)

    Tatsch, K. [Klinik und Poliklinik fuer Nuklearmedizin, Klinikum der Univ. Muenchen-Grosshardern (Germany)

    2002-09-01

    Stimulated by the commercial availability of specific radioligands in vivo characterization of the dopaminergic system with single-photon emission tomography (SPECT) has gained clinical importance in the diagnostic work-up of patiens with parkinsonism. Currently interest is focused on two aspects of the dopaminergic neurotransmission: Cocaine analogues bind to the presynaptically located striatal dopamine transporter and herewith allow to assess the structural integrity of the presynaptic terminals which are the striatal projections of neurons originating in the substantia nigra. For functional assessment of the postsynaptic aspect of the dopaminergic synapse binding of specific receptor antagonists to postsynaptically located D2 receptors is analyzed. Depending on the clinical question and the pathology expected both methods - either each one alone or a combination of both - provide valuable diagnostic information. Currently those SPECT methods are applied to confirm or exclude a Parkinsonian syndrome, in the early and differential diagnosis of Parkinsonian syndromes, to assess disease severity and measure disease progression, and to monitor the effects of therapy e.g. with potentially neuroprotective drugs. This paper offers a comprehensive summary of the SPECT results reported in the literature dealing with the mentioned clinical applications. (orig.) [German] Stimuliert durch die kommerzielle Verfuegbarkeit spezifischer Radioliganden gewinnt die In-vivo-Charakterisierung des dopaminergen Systems mit der single-photon-emissions-computertomographie (SPECT) bei der diagnostischen Abklaerung von Parkinson-Syndromen zunehmend an Bedeutung. Hierbei stehen zwei Aspekte der dopaminergen Neurotransmission im Blickpunkt des Interesses: Die Bindung von Kokainanaloga an den praesynaptischen Dopamintransporter laesst Rueckschluesse auf die Integritaet von Neuronen der Substantia nigra mit ihren zum Corpus striatum projizierenden Axonen (praesynaptische Nervenfasern) zu. Die

  14. Advances in SPECT and PET Hardware.

    Science.gov (United States)

    Slomka, Piotr J; Pan, Tinsu; Berman, Daniel S; Germano, Guido

    2015-01-01

    There have been significant recent advances in single photon emission computed tomography (SPECT) and positron emission tomography (PET) hardware. Novel collimator designs, such as multi-pinhole and locally focusing collimators arranged in geometries that are optimized for cardiac imaging have been implemented to reduce imaging time and radiation dose. These new collimators have been coupled with solid state photon detectors to further improve image quality and reduce scanner size. The new SPECT scanners demonstrate up to a 7-fold increase in photon sensitivity and up to 2 times improvement in image resolution. Although PET scanners are used primarily for oncological imaging, cardiac imaging can benefit from the improved PET sensitivity of 3D systems without inter-plane septa and implementation of the time-of-flight reconstruction. Additionally, resolution recovery techniques are now implemented by all major PET vendors. These new methods improve image contrast, image resolution, and reduce image noise. Simultaneous PET/magnetic resonance (MR) hybrid systems have been developed. Solid state detectors with avalanche photodiodes or digital silicon photomultipliers have also been utilized in PET. These new detectors allow improved image resolution, higher count rate, as well as a reduced sensitivity to electromagnetic MR fields. Copyright © 2015. Published by Elsevier Inc.

  15. Creation and evaluation of complementary composite three-dimensional image in various brain diseases. An application of three-dimensional brain SPECT image and three-dimensional CT image

    Energy Technology Data Exchange (ETDEWEB)

    Seiki, Yoshikatsu; Shibata, Iekado; Mito, Toshiaki; Sugo, Nobuo [Toho Univ., Tokyo (Japan). School of Medicine

    2000-09-01

    The purpose of this study was to develop 3D composite images for use in functional and anatomical evaluation of various cerebral pathologies. Imaging studies were performed in normal volunteers, patients with hydrocephalus and patients with brain tumor (meningioma and metastatic tumor) using a three-detector SPECT system (Prism 3000) and helical CT scanner (Xvigor). {sup 123}I-IMP was used in normal volunteers and patients with hydrocephalus, and {sup 201}TLCL in patients with brain tumor. An Application Visualization System-Medical Viewer (AVS-MV) was used on a workstation (Titan 2) to generate 3D images. A new program was developed by synthesizing surface rendering and volume rendering techniques. The clinical effects of shunt operations were successfully evaluated in patients with hydrocephalus by means of translucent 3D images of the deep brain. Changes in the hypoperfusion area around the cerebral ventricle were compared with morphological changes in the cerebral ventricle on CT. In addition to the information concerning the characteristics of brain tumors and surrounding edemas, hemodynamic changes and changeable hypoperfusion areas around the tumors were visualized on 3D composite CT and SPECT images. A new method of generating 3D composite images of CT and SPECT was developed by combining graphic data from different systems on the same workstation. Complementary 3D composite images facilitated quantitative analysis of brain volume and functional analysis in various brain diseases. (author)

  16. A Newly Designed 14.5 GHz All Permanent ECR Ion Source in IMP

    Institute of Scientific and Technical Information of China (English)

    SunLiangting; ZhaoHongwei; ZhangZimin; WangHui; MaBaohua; D.Hitz

    2003-01-01

    A new all permanent ECR (Electron Cyclotron Resonance) ion source LAPECR2 (Lanzhou All Permanent ECRIS No. 2) is now under developing in IMP. This source will be used to set up on thc IMP 400 HV (High Voltage) platform. This HV platform aims to deliver high ion beams from low charge state to very high charge state, so the rigorous requirement to this ECRIS is obvious. To satisfy this requirement, the ion source is designed to be a very large one, which has very large volumc plasma chamber, larger ECR length and mirror length, and very strong 3 -dimension magnetic field. The detail parameters, are shown in Table 1 listed below.

  17. Metabolic engineering of the purine biosynthetic pathway in Corynebacterium glutamicum results in increased intracellular pool sizes of IMP and hypoxanthine

    Directory of Open Access Journals (Sweden)

    Peifer Susanne

    2012-10-01

    Full Text Available Abstract Background Purine nucleotides exhibit various functions in cellular metabolism. Besides serving as building blocks for nucleic acid synthesis, they participate in signaling pathways and energy metabolism. Further, IMP and GMP represent industrially relevant biotechnological products used as flavor enhancing additives in food industry. Therefore, this work aimed towards the accumulation of IMP applying targeted genetic engineering of Corynebacterium glutamicum. Results Blocking of the degrading reactions towards AMP and GMP lead to a 45-fold increased intracellular IMP pool of 22 μmol gCDW-1. Deletion of the pgi gene encoding glucose 6-phosphate isomerase in combination with the deactivated AMP and GMP generating reactions, however, resulted in significantly decreased IMP pools (13 μmol gCDW-1. Targeted metabolite profiling of the purine biosynthetic pathway further revealed a metabolite shift towards the formation of the corresponding nucleobase hypoxanthine (102 μmol gCDW-1 derived from IMP degradation. Conclusions The purine biosynthetic pathway is strongly interconnected with various parts of the central metabolism and therefore tightly controlled. However, deleting degrading reactions from IMP to AMP and GMP significantly increased intracellular IMP levels. Due to the complexity of this pathway further degradation from IMP to the corresponding nucleobase drastically increased suggesting additional targets for future strain optimization.

  18. Relations between neuropsychological findings and lateral asymmetries of cerebral blood flow measured by SPECT in dementia of Alzheimer type

    Energy Technology Data Exchange (ETDEWEB)

    Yoshimura, Nahoko; Soma, Yoshiaki; Ootsuki, Mika [Takeda General Hospital, Aizu-Wakamatsu, Fukushima (Japan)

    1993-10-01

    We studied 16 right-handed patients clinically diagnosed as dementia of Alzheimer type (6 men, 10 women; aged 63-85, mean 72.8 years). The average duration of symptoms was 2.7 years. Dementia ranged from mild to moderately severe. None had clinical or laboratory evidence of cerebrovascular disease (Hachinski ischemic scores for all patients were 4 or below 4). All received the Wechsler Adult Intelligence Scale (WAIS), Mini-mental State Test (MMS) and Western Aphasia Battery (WAB, First Japanese edition, 1986). Regional cerebral blood flow (rCBF) was evaluated by single photon emission CT (SPECT) with {sup 123}I-N-isopropyl-p-iodoamphetamine ({sup 123}I-IMP), using the Matsuda`s quantitative method. Regional tracer uptake was measured in regions of interests (ROIs) over right and left frontal, temporal, parietal and occipital cortical regions; basal ganglia; and cerebellar hemispheres. The subjects were divided into three groups on the basis of lateral asymmetries in the temporal and parietal cortexes of rCBF (leftSPECT was related significantly to the deficit of language and constructive function in patients with dementia of Alzheimer type. Decreased rCBF in the left temporoparietal lobe was associated with language dysfunction, and that in the right hemisphere, with constructive dysfunction. (author).

  19. Quantification of perfusion and risk stratification by myocardial perfusion SPECT; Quantifizierung der Perfusion und Risikostratifizierung durch die Myokardperfusions-SPECT

    Energy Technology Data Exchange (ETDEWEB)

    Nowak, Bernd [Ueberoertliche Gemeinschaft (GbR) fuer diagnostische und interventionelle Radiologie und Nuklearmedizin (DIRANUK), Bielefeld (Germany); Klinikum Bielefeld-Mitte (Germany). Klinik fuer Nuklearmedizin; Schaefer, W.M. [Kliniken Maria Hilf GmbH, Krankenhaus St. Franziskus, Moenchengladbach (Germany). Klinik fuer Nuklearmedizin

    2010-06-15

    Myocardial perfusion SPECT detects flow-limiting coronary artery disease with high sensitivity and specificity, enables semiquantification of severity and extensiveness of myocardial ischemia, and furthermore enables reliable assessment of future cardiac events independently of other clinical and diagnostic parameters. A normal myocardial perfusion SPECT is associated with a favorable prognosis and warrants restrictive patient management. Cardiac risk increases in relation to the severity of perfusion abnormalities. Differentiated analysis of quantitative parameters derived from myocardial perfusion SPECT provides effective risk stratification of patients with a large variety of risk factors. (orig.)

  20. SPECT/CT in the Diagnosis of Skull Base Osteomyelitis

    Energy Technology Data Exchange (ETDEWEB)

    Damle, Nishikant Avinash; Kumar, Rakesh; Kumar, Praveen; Jaganthan, Sriram; Patnecha, Manish; Bal, Chandrasekhar; Bandopadhyaya, Gurupad; Malhotra, Arun [All India Institute of Medical Sciences, New Delhi (India)

    2011-09-15

    Skull base osteomyelitis is a potentially fatal disease. We demonstrate here the utility of SPECT/CT in diagnosing this entity, which was not obvious on a planar bone scan. A {sup 99mT}c MDP bone scan with SPECT/CT was carried out on a patient with clinically suspected skull base osteomyelitis. Findings were correlated with contrast enhanced CT (CECT) and MRI. Planar images were equivocal, but SPECT/CT showed intense uptake in the body of sphenoid and petrous temporal bone as well as the atlas corresponding to irregular bone destruction on CT and MRI. These findings indicate that SPECT/CT may have an additional role beyond planar imaging in the detection of skull base osteomyelitis.

  1. High-resolution SPECT for small-animal imaging

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    This article presents a brief overview of the development of high-resolution SPECT for small-animal imaging. A pinhole collimator has been used for high-resolution animal SPECT to provide better spatial resolution and detection efficiency in comparison with a parallel-hole collimator. The theory of imaging characteristics of the pinhole collimator is presented and the designs of the pinhole aperture are discussed. The detector technologies used for the development of small-animal SPECT and the recent advances are presented. The evolving trend of small-animal SPECT is toward a multi-pinhole and a multi-detector system to obtain a high resolution and also a high detection efficiency.

  2. Impact of reconstruction parameters on quantitative I-131 SPECT

    NARCIS (Netherlands)

    van Gils, C A J; Beijst, C; van Rooij, R; de Jong, H W A M

    2016-01-01

    Radioiodine therapy using I-131 is widely used for treatment of thyroid disease or neuroendocrine tumors. Monitoring treatment by accurate dosimetry requires quantitative imaging. The high energy photons however render quantitative SPECT reconstruction challenging, potentially requiring accurate cor

  3. Application of PET/SPECT imaging in vascular disease

    NARCIS (Netherlands)

    van der Vaart, M. G.; Meerwaidt, R.; Slart, R. H. J. A.; van Dam, G. M.; Tio, R. A.; Zeebregts, C. J.

    2008-01-01

    Background. Nuclear medicine imaging differs from other imaging modalities by showing physiological processes instead of anatomical details. Objective. To describe the current applications of positron emission tomography (PET) and single photon emission computed tomography (SPECT) as a diagnostic to

  4. Performance evaluation of advanced industrial SPECT system with diverging collimator.

    Science.gov (United States)

    Park, Jang Guen; Jung, Sung-Hee; Kim, Jong Bum; Moon, Jinho; Yeom, Yeon Soo; Kim, Chan Hyeong

    2014-12-01

    An advanced industrial SPECT system with 12-fold-array diverging collimator was developed for flow visualization in industrial reactors and was discussed in the previous study. The present paper describes performance evaluation of the SPECT system under both static- and dynamic- flow conditions. Under static conditions, the movement of radiotracer inside the test reactor was compared with that of color tracer (blue ink) captured with a high-speed camera. The comparison of the reconstructed images obtained with the radiotracer and the SPECT system showed fairly good agreement with video-frames of the color tracer obtained with the camera. Based on the results of the performance evaluation, it is concluded that the SPECT system is suitable for investigation and visualization of flows in industrial flow reactors.

  5. Impact of reconstruction parameters on quantitative I-131 SPECT

    NARCIS (Netherlands)

    van Gils, C A J; Beijst, C; van Rooij, R; de Jong, H W A M

    2016-01-01

    Radioiodine therapy using I-131 is widely used for treatment of thyroid disease or neuroendocrine tumors. Monitoring treatment by accurate dosimetry requires quantitative imaging. The high energy photons however render quantitative SPECT reconstruction challenging, potentially requiring accurate

  6. The value of SPECT/CT in carpal boss

    Energy Technology Data Exchange (ETDEWEB)

    Bhure, Ujwal; Huellner, Martin W.; Gruenig, Hannes; Zander, Andrea; Sol Perez Lago, Maria del; Strobel, Klaus [Cantonal Hospital Lucerne, Nuclear Medicine and Radiology, Lucerne (Switzerland); Hug, Urs [Cantonal Hospital Lucerne, Department of Hand and Plastic Surgery, Lucerne (Switzerland)

    2015-11-15

    To assess the value of SPECT/CT in patients with carpal boss (CB). In 24 wrists with CB (18 right-sided, 6 left-sided) in 21 patients, planar images and SPECT/CT images were obtained. Three patients had bilateral CB. The grade of uptake (0 absent, 1 low, 2 moderate, 3 high) on planar imaging and SPECT/CT was assessed and compared with CT findings, clinical symptoms and follow-up findings. CB affected carpometacarpal joint II in 4 wrists, carpometacarpal joint III in 17 wrists and both carpometacarpal joints II and III in 3 wrists. Of the 24 CB, 12 (50 %) were active (i.e. increased radionuclide uptake) on planar images and 18 (75 %) on SPECT/CT images. Of the 17 symptomatic CB, 10 (59 %) were active on planar images and 14 (82 %) were active (mean grade 1.9, range 1 - 3) on SPECT/CT images. Of the 7 asymptomatic CB, 2 were active on planar images and 4 were active (mean grade 1.25, range 1 - 2) on SPECT/CT images. CT alone showed CB in all patients and an accessory ossicle (os styloideum) in 8 wrists. MR imaging was available in 7 patients and positive for CB in 5 (sensitivity 71 %). Plain radiographs were available in 17 patients and positive in 10 (59 %). Therapeutic infiltration of the CB was performed in 9 patients, and resection of the CB in 7 patients. SPECT/CT provides important morphological and metabolic information for the clinical assessment of CB, but because SPECT/CT tends to overestimate the clinical importance of CB, we recommend that planar images should still be obtained. (orig.)

  7. A review on the clinical uses of SPECT/CT

    Energy Technology Data Exchange (ETDEWEB)

    Mariani, Giuliano; Bruselli, Laura [University of Pisa Medical School, Regional Center of Nuclear Medicine, Pisa (Italy); Kuwert, Torsten [Friedrich-Alexander-University, Clinic of Nuclear Medicine, Erlangen-Nuremberg (Germany); Kim, Edmund E. [MD Anderson Cancer Center, Nuclear Medicine Service, Houston, TX (United States); Flotats, Albert [Universitat Autonoma de Barcelona, Hospital de Sant Pau, Nuclear Medicine Department, Barcelona (Spain); Israel, Ora [Rambam Health Care Campus, Department of Nuclear Medicine, Haifa (Israel); Dondi, Maurizio [International Atomic Energy Agency, Nuclear Medicine Section, Division of Human Health, Vienna (Austria); Watanabe, Naoyuki [International Atomic Energy Agency, Nuclear Medicine Section, Division of Human Health, Vienna (Austria); Gunma Prefectural College of Health Sciences, Department of Radiological Technology, Gunma (Japan)

    2010-10-15

    In the era when positron emission tomography (PET) seems to constitute the most advanced application of nuclear medicine imaging, still the conventional procedure of single photon emission computed tomography (SPECT) is far from being obsolete, especially if combined with computed tomography (CT). In fact, this dual modality imaging technique (SPECT/CT) lends itself to a wide variety of useful diagnostic applications whose clinical impact is in most instances already well established, while the evidence is growing for newer applications. The increasing availability of new hybrid SPECT/CT devices with advanced technology offers the opportunity to shorten acquisition time and to provide accurate attenuation correction and fusion imaging. In this review we analyse and discuss the capabilities of SPECT/CT for improving sensitivity and specificity in the imaging of both oncological and non-oncological diseases. The main advantages of SPECT/CT are represented by better attenuation correction, increased specificity, and accurate depiction of the localization of disease and of possible involvement of adjacent tissues. Endocrine and neuroendocrine tumours are accurately localized and characterized by SPECT/CT, as also are solitary pulmonary nodules and lung cancers, brain tumours, lymphoma, prostate cancer, malignant and benign bone lesions, and infection. Furthermore, hybrid SPECT/CT imaging is especially suited to support the increasing applications of minimally invasive surgery, as well as to precisely define the diagnostic and prognostic profile of cardiovascular patients. Finally, the applications of SPECT/CT to other clinical disorders or malignant tumours is currently under extensive investigation, with encouraging results in terms of diagnostic accuracy. (orig.)

  8. Comparison of heterogeneity quantification algorithms for brain SPECT perfusion images

    OpenAIRE

    Modzelewski, Romain; Janvresse, Elise; De La Rue, Thierry; Vera, Pierre

    2012-01-01

    Background Several algorithms from the literature were compared with the original random walk (RW) algorithm for brain perfusion heterogeneity quantification purposes. Algorithms are compared on a set of 210 brain single photon emission computed tomography (SPECT) simulations and 40 patient exams. Methods Five algorithms were tested on numerical phantoms. The numerical anthropomorphic Zubal head phantom was used to generate 42 (6 × 7) different brain SPECT simulations. Seven diffuse cortical ...

  9. Cervical SPECT Camera for Parathyroid Imaging

    Energy Technology Data Exchange (ETDEWEB)

    None, None

    2012-08-31

    Primary hyperparathyroidism characterized by one or more enlarged parathyroid glands has become one of the most common endocrine diseases in the world affecting about 1 per 1000 in the United States. Standard treatment is highly invasive exploratory neck surgery called Parathyroidectomy. The surgery has a notable mortality rate because of the close proximity to vital structures. The move to minimally invasive parathyroidectomy is hampered by the lack of high resolution pre-surgical imaging techniques that can accurately localize the parathyroid with respect to surrounding structures. We propose to develop a dedicated ultra-high resolution (~ 1 mm) and high sensitivity (10x conventional camera) cervical scintigraphic imaging device. It will be based on a multiple pinhole-camera SPECT system comprising a novel solid state CZT detector that offers the required performance. The overall system will be configured to fit around the neck and comfortably image a patient.

  10. Individual Dose Monitor of External Radiation Personnel in IMP (1996~2001)

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    For evaluating the individual annual effective dose of eternal radiation personnel in IMP, we monitored individual dose of external radiation personnel every year. The monitoring results are shown in Table 1, from which it is known from 1998 to 2001, we monitored 1099 workers, the mean annual effective dose is 0.13 mSv.

  11. Uses of permanent plugging agent imp-op-301, polyacrylamide base, in oil-field production

    Energy Technology Data Exchange (ETDEWEB)

    Islas S, C.; Ledesma S, A.

    1974-10-01

    Due to its chemical and physical properties, the use of synthetic polymer gels has recently become an important auxiliary tool in petroleum production techniques. This paper described the properties of a new permanent plugging agent (IMP-OP-301), as well as the description of laboratory evaluation tests and some possible field applications of this acrylamide base product. (10 refs.)

  12. Therapeutic techniques applied in the heavy-ion therapy at IMP

    Science.gov (United States)

    Li, Qiang; Sihver, Lembit

    2011-04-01

    Superficially-placed tumors have been treated with carbon ions at the Institute of Modern Physics (IMP), Chinese Academy of Sciences (CAS), since November 2006. Up to now, 103 patients have been irradiated in the therapy terminal of the heavy ion research facility in Lanzhou (HIRFL) at IMP, where carbon-ion beams with energies up to 100 MeV/u can be supplied and a passive beam delivery system has been developed and commissioned. A number of therapeutic and clinical experiences concerning heavy-ion therapy have been acquired at IMP. To extend the heavy-ion therapy project to deep-seated tumor treatment, a horizontal beam line dedicated to this has been constructed in the cooling storage ring (CSR), which is a synchrotron connected to the HIRFL as an injector, and is now in operation. Therapeutic high-energy carbon-ion beams, extracted from the HIRFL-CSR through slow extraction techniques, have been supplied in the deep-seated tumor therapy terminal. After the beam delivery, shaping and monitoring devices installed in the therapy terminal at HIRFL-CSR were validated through therapeutic beam tests, deep-seated tumor treatment with high-energy carbon ions started in March 2009. The therapeutic techniques in terms of beam delivery system, conformal irradiation method and treatment planning used at IMP are introduced in this paper.

  13. ACR testing of a dedicated head SPECT unit.

    Science.gov (United States)

    Sensakovic, William F; Hough, Matthew C; Kimbley, Elizabeth A

    2014-07-08

    Physics testing necessary for program accreditation is rigorously defined by the ACR. This testing is easily applied to most conventional SPECT systems based on gamma camera technology. The inSPira HD is a dedicated head SPECT system based on a rotating dual clamshell design that acquires data in a dual-spiral geometry. The unique geometry and configuration force alterations of the standard ACR physics testing protocol. Various tests, such as intrinsic planar uniformity and/or resolution, do not apply. The Data Spectrum Deluxe Phantom used for conventional SPECT testing cannot fit in the inSPira HD scanner bore, making (currently) unapproved use of the Small Deluxe SPECT Phantom necessary. Matrix size, collimator type, scanning time, reconstruction method, and attenuation correction were all varied from the typically prescribed ACR instructions. Visible spheres, sphere contrast, visible rod groups, uniformity, and root mean square (RMS) noise were measured. The acquired SPECT images surpassed the minimum ACR requirements for both spatial resolution (9.5 mm spheres resolved) and contrast (6.4 mm rod groups resolved). Sphere contrast was generally high. Integral uniformity was 4% and RMS noise was 1.7%. Noise appeared more correlated than in images from a conventional SPECT scanner. Attenuation-corrected images produced from direct CT scanning of the phantom and a manufacturer supplied model of the phantom demonstrated negligible differences.

  14. Tc-99m Ciprofloxacin SPECT of Pulmonary Tuberculosis

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Min Kyung; Hwang, Kyung Hoon [Gachon University Gil Hospital, Incheon (Korea, Republic of); Yoon, Min Ki [Good Samaritan Hospital, Pohang (Korea, Republic of); Choe, Won Sick [Kangbuk Samsung Hospital, Seoul (Korea, Republic of)

    2010-06-15

    Tc-99m ciprofloxacin is available for imaging infection. However, there has been no study on employing single photon emission computed tomography (SPECT) with using Tc-99m ciprofloxacin to image active pulmonary tuberculosis. Therefore, we conducted this study to assess the efficacy of Tc-99m ciprofloxacin SPECT for imaging active pulmonary tuberculosis. Twenty-one participants were enrolled in this prospective study. They were divided into two groups according to the clinical and radiological assessment. Group one (Gr. 1) consisted of five normal volunteers and six patients with inactive pulmonary tuberculosis. Group two (Gr. 2) consisted of ten patients with active pulmonary tuberculosis. SPECT was performed 3 h after injecting 555 MBq (15 mCi) of Tc-99m ciprofloxacin. The findings of Tc-99m ciprofloxacin SPECT were interpreted by a nuclear medicine specialist and then the results were analyzed according to the patients' clinical and radiological classifications. The results of Tc-99m ciprofloxacin SPECT were as follows: eight true-positive cases, ten true-negative cases, one false-positive case and two false-negative cases. The sensitivity and specificity was 80.0% and 90.0%, respectively. The positive predictive value was 88.9% and the negative predictive value was 83.3%. Conclusions Tc-99m ciprofloxacin SPECT is feasible for imaging active pulmonary tuberculosis. It is a useful nuclear-imaging method for discriminating between the active and inactive tuberculosis states in patients with a past medical history of pulmonary tuberculosis.

  15. Evaluation of SPECT imaging using myocardial phantoms in Akita prefecture

    Energy Technology Data Exchange (ETDEWEB)

    Tamura, Kiyohiko [Akita Univ. (Japan). Hospital; Watarai, Jiro; Miura, Mamoru

    1998-09-01

    Evaluation of SPECT imaging using myocardial phantom in Akita Prefecture. The Society of Nuclear Medicine for Circulation disease in Akita was established in July, 1997. To improve myocardial spect imaging in Akita Prefecture, we first visually evaluated two acrlic defect (2 cm{phi} x 1 cm thickness aqcliel and 1 cm{phi} x 1 cm thickness) images of long axis and short axis of myocardial phantoms, using 14 SPECT Cameras. These defect images of myocardial phantom were evaluated by four cardiologists and twelve radiologists between August and December, 1996. Secondly, we measured the FWHM of four line sources (anterior, lateral, inferior, and septum positions in the short axis of myocardial phantom) using quantitative analysis by myocardial phantom between April and July, 1997. The results were reported at the 4th and 5th meeting of the Society of Nuclear Medicine for Circulation Disease in Akita. In conclusion, about 70% of myocardial spect images were of good or normal quality, whereas about 30% of the images were evaluated as of bad quality. To improve the myocardial spect images, we recognized that the basic performance of the SPECT cameras need be investigated. (author)

  16. PET/SPECT imaging: From carotid vulnerability to brain viability

    Energy Technology Data Exchange (ETDEWEB)

    Meerwaldt, Robbert [Department of Surgery, Isala Clinics, Zwolle (Netherlands); Slart, Riemer H.J.A. [Department of Nuclear Medicine and Molecular Imaging, University Medical Center Groningen, Groningen (Netherlands); Dam, Gooitzen M. van [Department of Surgery, University Medical Center Groningen, Groningen (Netherlands); Luijckx, Gert-Jan [Department of Neurology, University Medical Center Groningen, Groningen (Netherlands); Tio, Rene A. [Department of Cardiology, University Medical Center Groningen, Groningen (Netherlands); Zeebregts, Clark J. [Department of Surgery, University Medical Center Groningen, Groningen (Netherlands)], E-mail: czeebregts@hotmail.com

    2010-04-15

    Background: Current key issues in ischemic stroke are related to carotid plaque vulnerability, brain viability, and timing of intervention. The treatment of ischemic stroke has evolved into urgent active interventions, as 'time is brain'. Functional imaging such as positron emission tomography (PET)/single photon emission computed tomography (SPECT) could improve selection of patients with a vulnerable plaque and evaluation of brain viability in ischemic stroke. Objective: To describe the current applications of PET and SPECT as a diagnostic tool in relation to ischemic stroke. Methods: A literature search using PubMed identified articles. Manual cross-referencing was also performed. Results: Several papers, all observational studies, identified PET/SPECT to be used as a tool to monitor systemic atheroma modifying treatment and to select high-risk patients for surgery regardless of the degree of luminal stenosis in carotid lesions. Furthermore, PET/SPECT is able to quantify the penumbra region during ischemic stroke and in this way may identify those patients who may benefit from timely intervention. Discussion: Functional imaging modalities such as PET/SPECT may become important tools for risk-assessment and evaluation of treatment strategies in carotid plaque vulnerability and brain viability. Prospective clinical studies are needed to evaluate the diagnostic accuracy of PET/SPECT.

  17. 牛源犬新孢子虫IMP1基因的克隆与序列分析%Cloning and Sequence Analysis of Bovine Neospora caninum IMP1 Gene

    Institute of Scientific and Technical Information of China (English)

    张蕾; 贾立军; 鲁承

    2014-01-01

    [目的]为了解牛源犬新孢子虫IMP1基因生物学特性,分析牛源犬新孢子虫IMP1基因的克隆与序列.[方法]应用PCR技术扩增IMP1基因,将纯化的PCR产物和pMD18-T Simple Vector连接,构建pMD18-IMP1重组克隆质粒,进行PCR鉴定和酶切鉴定,将鉴定为阳性的质粒进行测序分析.[结果] PCR扩增犬新孢子虫IMP1基因大小为762 bp,克隆质粒经测序分析与GenBank (XM_003879531.1)中IMP1基因的同源性为99.9%.[结论]该试验为犬新孢子虫IMP1基因的表达及后续研究奠定了基础.

  18. Importance of SPECT/CT for resolving diseases of the jaw; Stellenwert der SPECT/CT zur Abklaerung von Kiefererkrankungen

    Energy Technology Data Exchange (ETDEWEB)

    Strobel, K.; Huellner, M.W.; Bolouri, C.; Veit-Haibach, P.; Sol Perez-Lago, M. del [Luzerner Kantonsspital, Institut fuer Nuklearmedizin und Roentgendiagnostik, Luzern (Switzerland); Merwald, M.; Kuttenberger, J. [Luzerner Kantonsspital, Klinik fuer Mund-, Kiefer- und Gesichtschirurgie, Luzern (Switzerland)

    2012-07-15

    Diseases of the jaw, such as osteomyelitis, condylar hyperactivity and tumors need adequate imaging to evaluate the extension and activity for therapy planning. Conventional planar scintigraphy, orthopantomography, computed tomography (CT) and magnetic resonance imaging (MRI) can be used for the evaluation of jaw diseases. Single photon emission computed tomography/computed tomography (SPECT/CT) provides metabolic and morphologic information in one imaging step and is becoming increasingly more available in larger hospitals. The SPECT/CT is superior to planar scintigraphy alone, CT and orthopantomography in the evaluation of the extension and activity of osteomyelitis and jaw tumors. In our hospital SPECT/CT has replaced the other imaging modalities in the evaluation of osteomyelitis and condylar hyperactivity. If available SPECT/CT should be performed for the evaluation of osteomyelitis of the jaw. (orig.) [German] Kiefererkrankungen wie Osteomyelitis, kondylaere Hyperaktivitaet und Kiefertumoren benoetigen eine exakte bildgebende Abklaerung, um die Therapie adaequat planen zu koennen. Die klassische planare Skelettszintigraphie, Orthopantomographie, CT und MRT koennen zur Abklaerung von Kiefererkrankungen eingesetzt werden. Die ''single photon emission computed tomography''/CT (SPECT/CT) bietet metabolische und morphologische Informationen in einem Untersuchungsgang und ist zunehmend in groesseren Kliniken verfuegbar. Die SPECT/CT ist zur Beurteilung der Ausbreitung und Aktivitaet einer Osteomyelitis und von Kiefertumoren der planaren Szintigraphie alleine, der CT und der Orthopantomographie ueberlegen. In unserer Klinik hat die SPECT/CT die bisherigen Verfahren zur Beurteilung der Kieferosteomyelitis und kondylaeren Hyperaktivitaet abgeloest. Sofern verfuegbar, sollte zur Abklaerung einer Kieferosteomyelitis die SPECT/CT eingesetzt werden. (orig.)

  19. Importance of SPECT/CT for knee and hip joint prostheses; Stellenwert der SPECT/CT bei Knie- und Hueftgelenkprothesen

    Energy Technology Data Exchange (ETDEWEB)

    Strobel, K.; Steurer-Dober, I.; Huellner, M.W.; Veit-Haibach, P.; Allgayer, B. [Luzerner Kantonsspital (Switzerland). Institut fuer Nuklearmedizin und Roentgendiagnostik

    2012-07-15

    Complications, such as loosening or infections are common problems after hip or knee arthroplasty. If conventional X-rays are equivocal bone scintigraphy is the classical second-line imaging modality. Single photon emission computed tomography/computed tomography (SPECT/CT) offers metabolic and morphologic information in one imaging step and is becoming increasingly more available in larger hospitals. The SPECT/CT procedure is a promising method and is increasingly being used in daily routine to evaluate joint arthroplasty. The additional benefit compared with classical conventional bone scintigraphy has to be evaluated in further prospective studies. In our hospital SPECT/CT regularly gives important additional information regarding prosthetic joint complications. SPECT/CT is increasingly being used as the second step imaging standard modality if conventional X-rays are equivocal. (orig.) [German] Komplikationen wie Lockerung und Infekt stellen ein haeufiges Problem nach Hueft- und Kniegelenkprothesen dar. Wenn die konventionelle Roentgenaufnahme nicht zum Ziel fuehrt, ist die klassische konventionelle Skelettszintigraphie die am haeufigsten verwendete ''Second-line''-Bildgebung. Die ''single photon emission computed tomography''/CT (SPECT/CT) bietet metabolische und morphologische Informationen bzgl. Prothesenkomplikationen in einem Untersuchungsgang und ist zunehmend in groesseren Kliniken verfuegbar. Die SPECT/CT ist eine viel versprechende Methode und wird im klinischen Alltag bei der Evaluation von Gelenkprothesen zunehmend eingesetzt. Es sind noch mehr prospektive Studien noetig, um die Leistungsfaehigkeit und den Zusatznutzen gegenueber der klassischen Szintigraphie zu evaluieren. In unserer Klinik wird die Knochenszintigraphie bei der Abklaerung von Prothesenkomplikationen zumeist mit einer SPECT/CT kombiniert und liefert regelmaessig wichtige Zusatzinformationen. Die SPECT/CT entwickelt sich zunehmend zum Standard

  20. Mechanism underlying the development of unilateral spatial neglect; A test of the network hypothesis by SPECT study

    Energy Technology Data Exchange (ETDEWEB)

    Nishikiori, Etsuko (Saitama Medical School, Moroyama (Japan))

    1992-10-01

    To test the hypothesis that functional disturbance of the neural network involving the inferior parietal lobule (IPL), anterior cingulate gyrus (ACG), dorsolateral frontal lobe (DLF), and thalamus (TH) as components of the right hemisphere underlies the development of unilateral spatial neglect (USN), cerebral perfusion was measured by [sup 123]I-IMP SPECT in 32 patients with cerebrovascular right brain damage, 20 of whom had USN and 12 of whom did not. In analyzing the SPECT data, RI uptake in the four component regions and cerebellum (serving as a control) were estimated by symmetrically placing 'regions of interest' from both hemispheres on SPECT slices, most suitable for each region. The 'regional to cerebellar ratio' (R/CE ratio) for each component region was calculated and the values were compared. In the USN group, R/CE ratio values for each component region in the right hemisphere were significantly lower than those in the left, whereas in the non-USN group there was no right-left difference. When R/CE ratio values for each component region in the right hemisphere were compared between the USN and non-USN group, those for the IPL, ACG and TH were significantly lower in the USN group; the value for the DLF was also lower in the USN group, although the difference was not significant. Significantly lower values of R/CE for each component region in the right hemisphere were noticed when the regions showed apparent involvement on X-ray CT/MRI. Furthermore, in seven of the USN patients where lesions revealed by CT/MRI did not involve network components, the R/CE ratio values for the components in the right hemisphere were lower than those in the left; the difference was significant for the IPL, ACG and TH, but not for the DLF. It is suggested that functional disturbance of the neural network involving the IPL, ACG, DLF and TH in the right hemisphere might underlie the development of USN. (author).

  1. Sequential SPECT/CT imaging starting with stress SPECT in patients with left bundle branch block suspected for coronary artery disease

    Energy Technology Data Exchange (ETDEWEB)

    Engbers, Elsemiek M.; Mouden, Mohamed [Isala, Department of Cardiology, Zwolle (Netherlands); Isala, Department of Nuclear Medicine, Zwolle (Netherlands); Timmer, Jorik R.; Ottervanger, Jan Paul [Isala, Department of Cardiology, Zwolle (Netherlands); Knollema, Siert; Jager, Pieter L. [Isala, Department of Nuclear Medicine, Zwolle (Netherlands)

    2017-01-15

    To investigate the impact of left bundle branch block (LBBB) on sequential single photon emission computed tomography (SPECT)/ CT imaging starting with stress-first SPECT. Consecutive symptomatic low- to intermediate-risk patients without a history of coronary artery disease (CAD) referred for SPECT/CT were included from an observational registry. If stress SPECT was abnormal, additional rest SPECT and, if feasible, coronary CT angiography (CCTA) were acquired. Of the 5,018 patients, 218 (4.3 %) demonstrated LBBB. Patients with LBBB were slightly older than patients without LBBB (65±12 vs. 61±11 years, p<0.001). Stress SPECT was more frequently abnormal in patients with LBBB (82 % vs. 46 %, p<0.001). After reviewing stress and rest images, SPECT was normal in 43 % of the patients with LBBB, compared to 77 % of the patients without LBBB (p<0.001). Sixty-four of the 124 patients with LBBB and abnormal stress-rest SPECT underwent CCTA (52 %), which could exclude obstructive CAD in 46 of the patients (72 %). Sequential SPECT/CT imaging starting with stress SPECT is not the optimal imaging protocol in patients with LBBB, as the majority of these patients have potentially false-positive stress SPECT. First-line testing using CCTA may be more appropriate in low- to intermediate-risk patients with LBBB. (orig.)

  2. Diagnostic role of (99)Tc(m)-MDP SPECT/CT combined SPECT/MRI Multi modality imaging for early and atypical bone metastases.

    Science.gov (United States)

    Chen, Xiao-Liang; Li, Qian; Cao, Lin; Jiang, Shi-Xi

    2014-01-01

    The bone metastasis appeared early before the bone imaging for most of the above patients. (99)Tc(m)-MDP ((99)Tc(m) marked methylene diphosphonate) bone imaging could diagnosis the bone metastasis with highly sensitivity, but with lower specificity. The aim of this study is to explore the diagnostic value of (99)Tc(m)-MDP SPECT/CT combined SPECT/MRI Multi modality imaging for the early period atypical bone metastases. 15 to 30 mCi (99)Tc(m)-MDP was intravenously injected to the 34 malignant patients diagnosed as doubtful early bone metastases. SPECT, CT and SPECT/CT images were captured and analyzed consequently. For the patients diagnosed as early period atypical bone metastases by SPECT/CT, combining the SPECT/CT and MRI together as the SPECT/MRI integrated image. The obtained SPECT/MRI image was analyzed and compared with the pathogenic results of patients. The results indicated that 34 early period doubtful metastatic focus, including 34 SPECT positive focus, 17 focus without special changes by using CT method, 11 bone metastases focus by using SPECT/CT method, 23 doubtful bone metastases focus, 8 doubtful bone metastases focus, 14 doubtful bone metastases focus and 2 focus without clear image. Totally, SPECT/CT combined with SPECT/MRI method diagnosed 30 bone metastatic focus and 4 doubtfully metastatic focus. In conclusion, (99)Tc(m)-MDP SPECT/CT combined SPECT/MRI Multi modality imaging shows a higher diagnostic value for the early period bone metastases, which also enhances the diagnostic accuracy rate.

  3. Semiquantitative regional cerebral blood flow evaluation using sup 123 I-IMP SPECT in a case showing transient ischemic attack caused by putaminal hemorrhage

    Energy Technology Data Exchange (ETDEWEB)

    Tsuda, Y.; Ayada, Y.; Takahashi, T.; Toyama, Y.; Matsuo, H. (Second Department of Internal Medicine, Kagawa Medical School (Japan)); Katsuragawa, M.; Tanabe, M. (Department of Radiology, Kagawa Medical School (Japan))

    1991-01-01

    A 69-year-old woman presented a transient cerebral ischemic attack, showing left arm weakness and slurred speech which recovered within 4 h of onset, while computed tomography indicated a putaminal hemorrhage. The regional cerebral blood flow distribution, measured semiquantitatively by use of {sup 123}I amphetamine emission tomography, was disturbed, which persisted more than one month up to a maximum of 4.5 months from the onset of symptoms. the case illustrates a variety of putaminal hemorrhage of good functional and vital prognosis, and provides and example in which the regional cerebral blood flow disturbances might persist for more than one month up to 4.5 months after the occurrence of a transient ischemic attack caused by a putaminal hemorrhage. (author).

  4. Proceedings of clinical SPECT (single photon emission computed tomography) symposium

    Energy Technology Data Exchange (ETDEWEB)

    1986-09-01

    It has been five years since the last in-depth American College of Nuclear Physicians/Society of Nuclear Medicine Symposium on the subject of single photon emission computed tomography (SPECT) was held. Because this subject was nominated as the single most desired topic we have selected SPECT imaging as the basis for this year's program. The objectives of this symposium are to survey the progress of SPECT clinical applications that have taken place over the last five years and to provide practical and timely guidelines to users of SPECT so that this exciting imaging modality can be fully integrated into the evaluation of pathologic processes. The first half was devoted to a consideration of technical factors important in SPECT acquisition and the second half was devoted to those organ systems about which sufficient clinical SPECT imaging data are available. With respect to the technical aspect of the program we have selected the key areas which demand awareness and attention in order to make SPECT operational in clinical practice. These include selection of equipment, details of uniformity correction, utilization of phantoms for equipment acceptance and quality assurance, the major aspect of algorithms, an understanding of filtered back projection and appropriate choice of filters and an awareness of the most commonly generated artifacts and how to recognize them. With respect to the acquisition and interpretation of organ images, the faculty will present information on the major aspects of hepatic, brain, cardiac, skeletal, and immunologic imaging techniques. Individual papers are processed separately for the data base. (TEM)

  5. Radiotracers for SPECT imaging. Current scenario and future prospects

    Energy Technology Data Exchange (ETDEWEB)

    Adak, S.; Vijaya Raj, K.K.; Mandal, S. [GE Healthcare Medical Diagnostics, John F. Welch Technology Center, Bangalore (India).; Bhalla, R.; Pickett, R.; Luthra, S.K. [GE Healthcare Medical Diagnostics, The Grove Centre, Amersham (United Kingdom)

    2012-07-01

    Single photon emission computed tomography (SPECT) has been the cornerstone of nuclear medicine and today it is widely used to detect molecular changes in cardiovascular, neurological and oncological diseases. While SPECT has been available since the 1980s, advances in instrumentation hardware, software and the availability of new radiotracers that are creating a revival in SPECT imaging are reviewed in this paper. The biggest change in the last decade has been the fusion of CT with SPECT, which has improved attenuation correction and image quality. Advances in collimator design, replacement of sodium iodide crystals in the detectors with cadmium zinc telluride (CZT) detectors as well as advances in software and reconstruction algorithms have all helped to retain SPECT as a much needed and used technology. Today, a wide spectrum of radiotracers is available for use in cardiovascular, neurology and oncology applications. The development of several radiotracers for neurological disorders is briefly described in this review, including [{sup 123}I]FP-CIT (DaTSCAN trademark) available for Parkinson's disease. In cardiology, while technetium-99m labeled tetrofosmin and technetium-99m labeled sestamibi have been well known for myocardial perfusion imaging, we describe a recently completed multicenter clinical study on the use of [{sup 123}I]mIBG (AdreView trademark) for imaging in chronic heart failure patients. For oncology, while bone scanning has been prevalent, newer radiotracers that target cancer mechanisms are being developed. Technetium-99m labeled RGD peptides have been reported in the literature that can be used for imaging angiogenesis, while technetium-99m labeled duramycin has been used to image apoptosis. While PET/CT is considered to be the more advanced technology particularly for oncology applications, SPECT continues to be the modality of choice and the workhorse in many hospitals and nuclear medicine centers. The cost of SPECT instruments also

  6. Sequential dimerization of human zipcode-binding protein IMP1 on RNA: a cooperative mechanism providing RNP stability

    DEFF Research Database (Denmark)

    Nielsen, J.; Kristensen, M. A.; Willemoes, Martin;

    2004-01-01

    zipcode-binding protein IMP1 on targets in the 3'-UTR from Igf-II mRNA and in H19 RNA. In both cases, two molecules of IMP1 bound to RNA by a sequential, cooperative mechanism, characterized by an initial fast step, followed by a slow second step. The first step created an obligatory assembly intermediate...... of low stability, whereas the second step was the discriminatory event that converted a putative RNA target into a ‘locked' stable RNP. The ability to dimerize was also observed between members of the IMP family of zipcode-binding proteins, providing a multitude of further interaction possibilities...

  7. Editorial. L’impôt européen : du verrou à la clé

    OpenAIRE

    Michel Dévoluy

    2008-01-01

    L’impôt européen : du verrou à la clé Je paye des impôts donc je suis citoyen. En effet, chacun s’identifie fortement à la collectivité dans laquelle il est un contribuable. L’impôt est un vecteur d’identité : il unit. Or les Européens, après plus de soixante ans d’histoire partagée, n’ont toujours pas un (...) ...

  8. Dual Isotope SPECT Study With Epilepsy Patients Using Semiconductor SPECT System.

    Science.gov (United States)

    Shiga, Tohru; Suzuki, Atsuro; Sakurai, Kotarou; Kurita, Tsugiko; Takeuchi, Wataru; Toyonaga, Takuya; Hirata, Kenji; Kobashi, Keiji; Katoh, Chietsugu; Kubo, Naoki; Tamaki, Nagara

    2017-09-01

    We developed a prototype CdTe SPECT system with 4-pixel matched collimator for brain study. This system provides high-energy-resolution (6.6%), high-sensitivity (220 cps/MBq/head), and high-spatial-resolution images. The aim of this study was to evaluate dual-isotope study of CBF and central benzodiazepine receptor (BZR) images using Tc-ECD and I-IMZ with the new SPECT system in patients with epilepsy comparing with single-isotope study using the conventional scintillation gamma camera. This study included 13 patients with partial epilepsy. The BZR images were acquired at 3 hours after I-IMZ injection for 20 minutes. The images of IMZ were acquired with a conventional 3-head scintillation gamma camera. After BZR image acquisition with the conventional camera, Tc-ECD was injected, and CBF and BZR images were acquired simultaneously 5 minutes after ECD injection with the new SPECT system. The CBF images were also acquired with the conventional camera on separate days. The findings were visually analyzed, and 3D-SSP maximum Z scores of lesions were compared between the 2 studies. There were 47 abnormal lesions on BZR images and 60 abnormal lesions on CBF images in the single-isotope study with the conventional camera. Dual-isotope study with the new system showed concordant abnormal findings of 46 of 47 lesions on BZR and 54 of 60 lesions on CBF images with the single-isotope study with the conventional camera. There was high agreement between the 2 studies in both BZR and CBF findings (Cohen κ values = 0.96 for BZR and 0.78 for CBF). In semiquantitative analysis, maximum Z scores of dual-isotope study with the new system strongly correlated with those of single-isotope study with the conventional camera (BZR: r = 0.82, P < 0.05, CBF: r = 0.87, P < 0.05). Our new SPECT system permits dual-isotope study for pixel-by-pixel analysis of CBF and BZR information with the same pathophysiological condition in patients with epilepsy.

  9. U-SPECT-II: An Ultra-High-Resolution Device for Molecular Small-Animal Imaging

    NARCIS (Netherlands)

    Van der Have, F.; Vastenhouw, B.; Ramakers, R.M.; Branderhorst, W.; Krah, J.O.; Ji, C.; Staelens, S.G.; Beekman, F.J.

    2009-01-01

    We present a new rodent SPECT system (U-SPECT-II) that enables molecular imaging of murine organs down to resolutions of less than half a millimeter and high-resolution total-body imaging. Methods: The U-SPECT-II is based on a triangular stationary detector set-up, an XYZ stage that moves the animal

  10. SPECT imaging of cardiac reporter gene expression in living rabbits

    Institute of Scientific and Technical Information of China (English)

    LIU Ying; LAN Xiaoli; ZHANG Liang; WU Tao; JIANG Rifeng; ZHANG Yongxue

    2009-01-01

    This work is to demonstrate feasibility of imaging the expression of herpes simplex virus 1-thymidine ki-nase (HSV1-tk) reporter gene in rabbits myocardium by using the reporter probe 131I-2'-fluoro-2'-deoxy-1-β-D- arabi-nofuranosyl-5-iodouracil (131I-FIAU) and SPECT. Rabbits of the study group received intramyocardial injection of Ad5-tk and control group received aseptic saline injection. Two sets of experiments were performed on the study group. Rabbits of the 1st set were injected with 131I-FIAU 600 μCi at Day 2 after intramyocardial transfection of Ad5-tk in 1×109, 5×108, 1×108, 5×107 and 1×107 pfu, and heart SPECT imaging was done at different hours. Rabbits of the 2nd were transferred various titers of Ad5-tk (1×109, 5×108, 1×108, 5×107, 1×107 pfu) to determine the threshold and optimal viral titer needed for detection of gene expression. Two days later, 131I-FIAU was injected and heart SPECT imaging was performed at 6, 24 and 48 h, before killing them for gamma counting of the hearts. Reverse tran-scription-polymerase chain reaction (RT-PCR) was used to verify the transferred HSV1-tk gene expression. Semi-quantitative analysis derived of region of interest (ROI) of SPECT images and RT-PCR images was performed and the relationship of SPECT images with ex vivo gamma counting and mRNA level were evaluated. SPECT images conformed 131I-FIAU accumulation in rabbits injected with Ad5-tk in the anterolateral wall. The optimal images qual-ity was obtained at 24~48 h for different viral titers. The highest radioactivity in the focal myocardium was seen at 6 h, and then declined with time. The threshold was 5×107 pfu of virus titer. The result could be set better in 1~5×108 pfu by SPECT analysis and gamma counting. ROI-derived semi-quantitative study on SPECT images correlated well with ex vivo gamma counting and mRNA levels from RT-PCR analysis. The HSV1-tk/131I-FIAU reporter gene/reporter probe system is feasible for cardiac SPECT reporter gene imaging

  11. Parallel-hole collimator concept for stationary SPECT imaging.

    Science.gov (United States)

    Pato, Lara R V; Vandenberghe, Stefaan; Zedda, Tiziana; Van Holen, Roel

    2015-11-21

    Parallel-hole SPECT collimators have traditionally been manufactured by stacking sheets of lead foil or by casting. These techniques significantly restrict our options in terms of collimator geometry. However, recent developments in metal additive manufacturing are making novel collimator designs possible, giving rise to new opportunities in SPECT imaging. In this paper we propose an innovative type of collimator for stationary SPECT, using parallel-holes whose collimation direction depends on their axial position. Its main advantage compared to current stationary SPECT systems (which are based on pinholes) is that, using only axial bed translations, we can achieve complete angular sampling of an increased portion of the transaxial area of the collimator bore. This allows the system to be much more compact than current stationary SPECT systems that image objects of the same size. We describe three possible designs, for full-body, brain and small-animal imaging, respectively, and test their feasibility using simulations. The system modeling method is validated against realistic Monte Carlo simulations, and then used in the evaluation of the systems' performances and reconstructions. The simulations show that the system is able to reconstruct objects occupying the predicted field of view ([Formula: see text] of the transaxial area of the bore) without sampling artifacts. In particular, we perform reconstructions from noisy projection data obtained for an activity and scanning time similar to standard protocols for the three applications, and the resulting images indicate the possibility of using the proposed systems in practice.

  12. Affordable CZT SPECT with dose-time minimization (Conference Presentation)

    Science.gov (United States)

    Hugg, James W.; Harris, Brian W.; Radley, Ian

    2017-03-01

    PURPOSE Pixelated CdZnTe (CZT) detector arrays are used in molecular imaging applications that can enable precision medicine, including small-animal SPECT, cardiac SPECT, molecular breast imaging (MBI), and general purpose SPECT. The interplay of gamma camera, collimator, gantry motion, and image reconstruction determines image quality and dose-time-FOV tradeoffs. Both dose and exam time can be minimized without compromising diagnostic content. METHODS Integration of pixelated CZT detectors with advanced ASICs and readout electronics improves system performance. Because historically CZT was expensive, the first clinical applications were limited to small FOV. Radiation doses were initially high and exam times long. Advances have significantly improved efficiency of CZT-based molecular imaging systems and the cost has steadily declined. We have built a general purpose SPECT system using our 40 cm x 53 cm CZT gamma camera with 2 mm pixel pitch and characterized system performance. RESULTS Compared to NaI scintillator gamma cameras: intrinsic spatial resolution improved from 3.8 mm to 2.0 mm; energy resolution improved from 9.8% to molecular imaging, coupled with optimal collimator and image reconstruction, result in minimized dose and exam time. With CZT cost improving, affordable whole-body CZT general purpose SPECT is expected to enable precision medicine applications.

  13. Enhancing the utility of prostascint SPECT scans for patient management.

    Science.gov (United States)

    Noz, Marilyn E; Chung, Grace; Lee, Benjamin Y; Maguire, Gerald Q; DeWyngaert, J Keith; Doshi, Jay V; Kramer, Elissa L; Murphy-Walcott, Antoinette D; Zeleznik, Michael P; Kwak, Noeun G

    2006-04-01

    This project investigated reducing the artifact content of In-ill ProstaScint SPECT scans for use in treatment planning and management. Forty-one patients who had undergone CT or MRI scans and simultaneous Tc-99m RBC/In-111 ProstaScint SPECT scans were included. SPECT volume sets, reconstructed using Ordered Set-Expectation Maximum (OS-EM) were compared against those reconstructed with standard Filtered Back projection (FBP). Bladder activity in Tc-99m scans was suppressed within an ellipsoidal volume. Tc-99m voxel values were subtracted from the corresponding In-111 after scaling based on peak activity within the descending aorta. The SPECT volume data sets were merged with the CT or MRI scans before and after processing. Volume merging, based both on visual assessment and statistical evaluation, was not affected. Thus iterative reconstruction together with bladder suppression and blood pool subtraction may improve the interpretation and utility of ProstaScint SPECT scans for patient management.

  14. A SPECT imager with synthetic collimation

    Science.gov (United States)

    Havelin, Ronan J.; Miller, Brian W.; Barrett, Harrison H.; Furenlid, Lars R.; Murphy, J. M.; Foley, Mark J.

    2013-09-01

    This work outlines the development of a multi-pinhole SPECT system designed to produce a synthetic-collimator image of a small field of view. The focused multi-pinhole collimator was constructed using rapid-prototyping and casting techniques. The collimator projects the field of view through forty-six pinholes when the detector is adjacent to the collimator. The detector is then moved further from the collimator to increase the magnification of the system. The amount of pinhole-projection overlap increases with the system magnification. There is no rotation in the system; a single tomographic angle is used in each system configuration. The maximum-likelihood expectation-maximization (MLEM) algorithm is implemented on graphics processing units to reconstruct the object in the field of view. Iterative reconstruction algorithms, such as MLEM, require an accurate model of the system response. For each system magnification, a sparsely-sampled system response is measured by translating a point source through a grid encompassing the field of view. The pinhole projections are individually identified and associated with their respective apertures. A 2D elliptical Gaussian model is applied to the pinhole projections on the detector. These coefficients are associated with the object-space location of the point source, and a finely-sampled system matrix is interpolated. Simulations with a hot-rod phantom demonstrate the efficacy of combining low-resolution non-multiplexed data with high-resolution multiplexed data to produce high-resolution reconstructions.

  15. Brain SPECT in neurology: A critical review; Hirn-SPECT in der Neurologie: Eine kritische Uebersicht

    Energy Technology Data Exchange (ETDEWEB)

    Bartenstein, P. [Technische Univ. Muenchen (Germany). Nuklearmedizinische Klinik und Poliklinik]|[Essen Univ. (Gesamthochschule) (Germany). Klinik und Poliklinik fuer Neurologie; Weiller, C. [Technische Univ. Muenchen (Germany). Nuklearmedizinische Klinik und Poliklinik]|[Essen Univ. (Gesamthochschule) (Germany). Klinik und Poliklinik fuer Neurologie

    1994-10-01

    SPECT of the brain with radiopharmaceuticals monitoring regional cerebral blood flow, receptor binding and metabolism offers a wide variety of potentially clinically useful applications. These can be found in neuro-oncology, epilepsy, cerebrovascular diseases, extrapyramidal disorders, dementia and the determination of brain death. The evaluation of the benefit of these methods in routine use requires a close cooperation between nuclear medicine physicians and neurologists, not only in the interpretation and analysis of the actual clinical studies, but also in the design and realization of clinically oriented research with large series of patients. (orig.) [Deutsch] Die SPECT-Untersuchung des Gehirnes bietet mit Radiopharmaka des regionalen zerebralen Blutflusses, Rezeptorliganden und Stoffwechselmarkern ein breites Spektrum diagnostischer Moeglichkeiten in der Neuro-Onkologie, die Epilepsiediagnostik, bei zerebrovaskulaeren Erkrankungen, extrapyramidalen Bewegungsstoerungen, dementiellen Syndromen und der Hirntodbestimmung. Zur Evaluierung klinisch sinnvoller Indikationen in der Routinediagnostik bedarf es einer engen Zusammenarbeit zwischen Nuklearmedizinern und Neurologen bei der Auswertung und Interpretation der Befunde, aber auch beim Design und der Durchfuehrung klinischer Studien mit ausreichend grossen Patientenzahlen. (orig.)

  16. IMP 8 observations of a poleward plasma circulation: Evidence of entry into the plasma mantle?

    Science.gov (United States)

    Kolpak, M. A.; Paularena, K. I.; Richardson, J. D.; Siscoe, G. L.

    Plasma data from IMP 8 are used to map the spatial structure of plasma flow in Earth's magnetosheath. Plasma and magnetic field data from instruments on ISEE-3 and WIND are used to normalize the IMP 8 magnetosheath data to upstream solar wind conditions. For the majority of the data, the magnitude and direction of plasma flow inside the magnetosheath agree well with gas-dynamic models. About 8% of the ISEE-3-normalized velocity vectors and 3% of the WIND-normalized velocity vectors exhibit a poleward circulation in the YZ plane. The observed circulation is consistent with predictions for flow within the plasma mantle. The occurrence frequency of these poleward flows increases further down the tail, consistent with predictions that the mantle width increases tailward. Densities are lower where this flow is observed, consistent with expectations for the plasma mantle, but temperatures are higher and the magnetic field magnitudes lower, the opposite of theoretical expectations.

  17. A propósito do conceito de império

    OpenAIRE

    Moita, Luís

    2005-01-01

    Após um breve panorama do debate em Portugal sobre a natureza imperial do poder norte-americano, o artigo recapitula a história dos termos “imperialismo” e “império” e distingue dois grandes tipos de impérios histó- ricos: os pré-modernos (civilizações agrárias) e os modernos (coloniais, ligados às sociedades industriais). Em referência à história dos Estados Unidos, mostra-se a relativa contradição entre a recusa imperial e o “destino manifesto” de uma expansão próxima da c...

  18. ICMEs Likely From the Same Active Region Observed by Both Helios 1 and IMP 8

    Institute of Scientific and Technical Information of China (English)

    DU Dan; WANG Chi

    2007-01-01

    The chance of an Interplanetary Coronal Mass Ejection (ICME) observed by widely-separated spacecraft is rare. However, such an event provides us a good opportunity to study the propagation and evolution of ICMEs in the heliosphere. On day 72 of 1975, an ICME was observed by Helios 1 at 0.3 AU, while a similar solar wind structure was observed by IMP 8 at Earth on day 70 of 1975. On the basis of comparison of the plasma signatures and the transit time from Helios 1 to IMP 8, we hypothesize the observed ICMEs by both spacecraft are resulted from the same active region on the solar surface. A one-dimensional MHD model was used to track the ICME from Helios 1 (0.3 AU) to Earth. The observed plasma profiles and timing are close to those predicted by our MHD model and thus, give the supports to the model.

  19. Deuses em festa: os grandes festivais religiosos do império novo

    OpenAIRE

    Filipe, Pedro Miguel dos Reis

    2012-01-01

    Tese de mestrado, História antiga, Universidade de Lisboa, Faculdade de Letras, 2012 O período egípcio conhecido como Império Novo estendeu-se desde 1560 a. C. até 1170 a. C. Foi uma era particularmente impressionante e é a mais bem conhecida pelo mundo contemporâneo, especialmente depois da descoberta por Howard Carter do túmulo de Tutankhamon, em 1922. O Império Novo produziu não só este jovem rei, como também a mulher-faraó Hatchepsut, Tutmés III, Amenhotep III, Ramsés II, e...

  20. Genetic polymorphisms of the AMPD1 gene and their correlations with IMP contents in Fast Partridge and Lingshan chickens.

    Science.gov (United States)

    Hu, Jin; Yu, Ping; Ding, Xiaoling; Xu, Minglong; Guo, Baoping; Xu, Yinxue

    2015-12-15

    The object of this study was to evaluate associations between the adenosine monophosphate deaminase 1 (AMPD1) gene polymorphisms and inosine monophosphate acid (IMP) contents of chicken to provide a molecular marker for breeding. Three single nucleotide polymorphisms (SNPs), g.4064G/A, g.5573A/G and g.6805G/A were detected in exons IV, VI, and VIII of the AMPD1 gene in Fast Partridge and Lingshan chickens, respectively. All were purine conversion and caused no alteration in amino acid sequence. Statistical analysis revealed that Lingshan chicken with the homozygous genotype AA at position 4064 and 6805 had a significantly greater IMP content than those with the GG genotype (Pchicken with the genotype GG at position 6805 had a significantly greater IMP content compared with those with the AA genotype (Pchickens. The results in our study suggest that SNP 6805A/G can be used as a possible candidate marker of IMP content of chicken.

  1. Expression of IMP1 enhances production of murine leukemia virus vector by facilitating viral genomic RNA packaging.

    Directory of Open Access Journals (Sweden)

    Yun Mai

    Full Text Available Murine leukemia virus (MLV-based retroviral vector is widely used for gene transfer. Efficient packaging of the genomic RNA is critical for production of high-titer virus. Here, we report that expression of the insulin-like growth factor II mRNA binding protein 1 (IMP1 enhanced the production of infectious MLV vector. Overexpression of IMP1 increased the stability of viral genomic RNA in virus producer cells and packaging of the RNA into progeny virus in a dose-dependent manner. Downregulation of IMP1 in virus producer cells resulted in reduced production of the retroviral vector. These results indicate that IMP1 plays a role in regulating the packaging of MLV genomic RNA and can be used for improving production of retroviral vectors.

  2. Expression of IMP1 enhances production of murine leukemia virus vector by facilitating viral genomic RNA packaging.

    Science.gov (United States)

    Mai, Yun; Gao, Guangxia

    2010-12-29

    Murine leukemia virus (MLV)-based retroviral vector is widely used for gene transfer. Efficient packaging of the genomic RNA is critical for production of high-titer virus. Here, we report that expression of the insulin-like growth factor II mRNA binding protein 1 (IMP1) enhanced the production of infectious MLV vector. Overexpression of IMP1 increased the stability of viral genomic RNA in virus producer cells and packaging of the RNA into progeny virus in a dose-dependent manner. Downregulation of IMP1 in virus producer cells resulted in reduced production of the retroviral vector. These results indicate that IMP1 plays a role in regulating the packaging of MLV genomic RNA and can be used for improving production of retroviral vectors.

  3. Cloning,prokaryotic expression and immunological identification of Toxo-plasma surface antigen IMP1%弓形虫表面抗原IMP1的克隆原核表达及免疫学鉴定

    Institute of Scientific and Technical Information of China (English)

    寇景轩; 赵桂华; 魏庆宽; 徐超; 朱嵩; 尹昆

    2015-01-01

    目的:克隆刚地弓形虫强毒RH株的表面抗原免疫作图蛋白1(Immune mapped protein⁃1,IMP1),并对其进行原核表达纯化、鉴定。方法采用逆转录法合成刚地弓形虫RH株的第一链cDNA,以此为模板,用PCR法扩增野生型IMP1基因的最大开放阅读框(ORF)序列,TA克隆测序鉴定后,插入原核表达载体pET28b中,构建重组表达载体pET28b⁃IMP1,经双酶切和测序鉴定后转化大肠杆菌E.coli BL21(DE3),经异丙基硫代⁃β⁃D⁃半乳糖苷(IPTG)诱导表达携带6×组氨酸标签的IMP1融合蛋白,改变温度、诱导时间和IPTG浓度以优化诱导表达条件并测定蛋白可溶性,通过镍离子螯合(Ni2+⁃NTA)亲和层析纯化IMP1融合蛋白,经Western blotting验证重组蛋白的特异性。结果在弓形虫RH株速殖子cD⁃NA中成功调取了IMP1基因的ORF序列,并通过TA克隆和测序鉴定了IMP1基因的正确性,在此基础上构建了原核表达重组质粒pET28b⁃IMP1,经双酶切和测序验证了重组载体的正确性,并通过条件筛选确定了IMP1的最佳表达条件为20℃下0.3 mmol/L IPTG诱导9 h。经镍柱亲和层析纯化后,IMP1全蛋白表达量和可溶性均较好,与镍柱填料有较高的亲和力,能实现高效纯化。经SDS⁃PAGE及Western blotting鉴定,IMP1具有良好的免疫活性。结论新型强毒弓形虫RH株的表面抗原IMP1可在大肠杆菌原核表达系统中高效表达,全长蛋白可溶,性状稳定。本研究为进一步制备IMP1多克隆抗体,进行后续的体内表达研究以及抗弓形虫感染亚单位疫苗的构建和IMP1晶体结构研究奠定了基础。%Objective To subclone express and identify the immune mapped protein 1 IMP1 which encodes a surface an⁃tigen of Toxoplasma gondii. Methods The cDNA of T. gondii RH strain was synthesized by reverse transcription PCR the IMP1 open reading frame ORF was amplified by PCR using the T

  4. Water Quality in the Integrated Mariculture Pond Systems (IMPS) at Makoba Bay, Zanzibar, Tanzania

    OpenAIRE

    Mmochi, A.J.; Mwandya, A.W.

    2003-01-01

    The Makoba integrated mariculture pond system project (IMPS) has been on-going since 1998. Except for a period from mid 1999 to 2001, various water quality parameters, namely temperature, salinity, pH, dissolved inorganic nutrients and dissolved oxygen concentration and saturation, have been monitored continuously. In 2002, measurement of sediment oxygen demand (SOD) and consumption of oxygen by sediments was initiated. Dissolved oxygen concentration varied from the highest mon...

  5. A New ECR Ion Source for Atomic Physics Research at IMP

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    A new Electron Cyclotron Resonance (ECR) ion source (LECR3-Lanzhou Electron Cyclotron Resonance ion source No.3) was constructed this year. The main purpose of this source is to provide highly charged ion beams for atomic physics and surface physics research. The design of this ion source is based on the IMP 14.5 GHz ECR ion source (LECR2-Lanzhou Electron Cyclotron Resonance ion source No.2) with double RF heating

  6. A new treatment of capillarity to improve the stability of IMPES two-phase flow formulation

    KAUST Repository

    Kou, Jisheng

    2010-12-01

    In this paper, we present an efficient numerical method for two-phase immiscible flow in porous media with different capillarity pressures. In highly heterogeneous permeable media, the saturation is discontinuous due to different capillary pressure functions. One popular scheme is to split the system into a pressure and a saturation equation, and to apply IMplicit Pressure Explicit Saturation (IMPES) approach for time stepping. One disadvantage of IMPES is instability resulting from the explicit treatment for capillary pressure. To improve stability, the capillary pressure is usually incorporated in the saturation equation which gradients of saturation appear. This approach, however, does not apply to the case of different capillary pressure functions for multiple rock-types, because of the discontinuity of saturation across rock interfaces. In this paper, we present a new treatment of capillary pressure, which appears implicitly in the pressure equation. Using an approximation of capillary function, we substitute the implicit saturation equation into the pressure equation. The coupled pressure equation will be solved implicitly and followed by the explicit saturation equation. Five numerical examples are provided to demonstrate the advantages of our approach. Comparison shows that our proposed method is more efficient and stable than the classical IMPES approach. © 2010 Elsevier Ltd.

  7. CircRNA-protein complexes: IMP3 protein component defines subfamily of circRNPs

    Science.gov (United States)

    Schneider, Tim; Hung, Lee-Hsueh; Schreiner, Silke; Starke, Stefan; Eckhof , Heinrich; Rossbach, Oliver; Reich, Stefan; Medenbach, Jan; Bindereif , Albrecht

    2016-01-01

    Circular RNAs (circRNAs) constitute a new class of noncoding RNAs in higher eukaryotes generated from pre-mRNAs by alternative splicing. Here we investigated in mammalian cells the association of circRNAs with proteins. Using glycerol gradient centrifugation, we characterized in cell lysates circRNA-protein complexes (circRNPs) of distinct sizes. By polysome-gradient fractionation we found no evidence for efficient translation of a set of abundant circRNAs in HeLa cells. To identify circRNPs with a specific protein component, we focused on IMP3 (IGF2BP3, insulin-like growth factor 2 binding protein 3), a known tumor marker and RNA-binding protein. Combining RNA-seq analysis of IMP3-co-immunoprecipitated RNA and filtering for circular-junction reads identified a set of IMP3-associated circRNAs, which were validated and characterized. In sum, our data suggest that specific circRNP families exist defined by a common protein component. In addition, this provides a general approach to identify circRNPs with a given protein component. PMID:27510448

  8. L’impératif en français parlé

    Directory of Open Access Journals (Sweden)

    Nguyen Minh Chinh

    2016-01-01

    Full Text Available Dans la grammaire française, l’impératif est considéré comme le moyen typique pour exprimer l’injonction. Il peut être utilisé pour tout but injonctif, du plus léger (prière, sollicitation au plus péremptoire (ordre, commandement. Cependant, l’impératif n’exprime pas toujours la valeur injonctive proprement dite, les locuteurs peuvent l’utiliser pour d’autres buts tels que l’expression de la condition ou de l’interrogation, etc. Partant de l’idée qu’à l’oral chaque genre a son vocabulaire de construction, ses formes d’organisation, ses contenus attendus, ses modes productifs, la caractérisation des genres est un préalable à la constitution de corpus utilisables pour des tâches de l’étude. Je présenterai dans cet article l’analyse des caractéristiques pragmatiques des énoncés impératifs et de leur répartition en m’appuyant sur l’analyse des occurrences retirées de 5 situations différentes.

  9. Corrections in clinical Magnetic Resonance Spectroscopy and SPECT

    DEFF Research Database (Denmark)

    de Nijs, Robin

    to the known mean and median filtering. The data comes from non-anesthetized preterm infants, where motion during scanning is a common problem. Both the novel outlier identification and the independent component analysis (ICA) perform satisfactory and better than the common mean and median filtering. ICA...... a detrimental effect of the extra-uterine environment on brain development. Paper II describes a method to correct for downscatter in low count Iodine-123 SPECT with a broad energy window above the normal imaging window. Both spatial dependency and weight factors were measured. As expected, the implicitly...... of the striatum due to downscatter correction. This makes the difference between healthy subjects and patients more profound. Downscatter in Iodine-123 SPECT is not the only deteriorating mechanism. Normal scatter compromises the images quality as well. Since scatter correction of SPECT-images also can...

  10. Clinical Significance of Brain SPECT in Zipeprol Abusers

    Energy Technology Data Exchange (ETDEWEB)

    Cho, Dai Ok; Kim, Jae Phil; Kim, Deog Yoon; Yang, Hyung In; Koh, Eun Mi; Kim, Kwang Won; Choi, Young Kil [Kyung Hee University College of Medicine, Seoul (Korea, Republic of)

    1993-03-15

    Drug abuse is widespread in worldwide and has been associated with neurologic complication. Zipeprol is one of drugs which been abused for psychological satisfaction in some adolescents. This agent is non-opioid antitussive agent, which is not legally considered as being capable of creating dependence or abuse liability at therapeutic serum levels. But it has been reported that acute or chronic overdose create neurologic complication such as convulsion as well as dependence. Recently we experienced six zipeprol abusers who admitted due to convulsion and variable neurologic symptoms. The aim of our study was to determine the role of Tc-99m- HMPAO brain SPECT in those patients. EEG and brain CT showed no abnormal finding, but brain SPECT showed focal or multiple perfusion abnormalities in frontal, parietal, occipital cortex, basal ganglia, thalamus and especially at temporal cortex. These results suggest that brain SPECT may be a useful diagnostic tool to evaluate the cerebral dysfunction induced by zipeprol abuse.

  11. SPECT-CT and PET-CT progress in the research of computer analysis method

    Directory of Open Access Journals (Sweden)

    Jingang Guo

    2015-04-01

    Full Text Available The development of multimodal imaging equipment is milestone in the development of imaging, after the PET-CT, American GE company launched a Discovery 670 NM/CT, CT and SPECT, the organic integration of the formation of SPECT-CT new molecular medical imaging equipment, with SPECT, CT and PET-CT is getting more and more widely attention and application, many of SPECT-CT and PET-CT image analysis computer method arises at the historic moment, getting increasing attention of the clinical and imaging science. The paper carried on the detailed description of the SPECT-CT and PET-CT computer analysis method.

  12. Determination of left ventricular mass through SPECT imaging

    Science.gov (United States)

    Zárate-Morales, A.; Rodríguez-Villafuerte, M.; Martínez-Rodríguez, F.; Arévila-Ceballos, N.

    1998-08-01

    An edge detection algorithm has been applied to estimate left ventricular (LV) mass from single photon emission computed tomography (SPECT) thallium-201 images. The algorithm was validated using SPECT images of a phantom. The algorithm was applied to 20 patient studies from the Hospital de Cardiologia, Centro Médico Nacional Siglo XXI. Left ventricular masses derived from the stress and redistribution studies were highly correlated (r=0.96). The average LV masses obtained were 162±37 g and 169±34 g in the redistribution and stress studies, respectively.

  13. Post-transcriptional regulation of cyclins D1, D3 and G1 and proliferation of human cancer cells depend on IMP-3 nuclear localization.

    Science.gov (United States)

    Rivera Vargas, T; Boudoukha, S; Simon, A; Souidi, M; Cuvellier, S; Pinna, G; Polesskaya, A

    2014-05-29

    RNA-binding proteins of the IMP family (insulin-like growth factor 2 (IGF2) mRNA-binding proteins 1-3) are important post-transcriptional regulators of gene expression. Multiple studies have linked high expression of IMP proteins, and especially of IMP-3, to an unfavorable prognosis in numerous types of cancer. The specific importance of IMP-3 for cancer transformation remains poorly understood. We here show that all three IMPs can directly bind the mRNAs of cyclins D1, D3 and G1 (CCND1, D3 and G1) in vivo and in vitro, and yet only IMP-3 regulates the expression of these cyclins in a significant manner in six human cancer cell lines of different origins. In the absence of IMP-3, the levels of CCND1, D3 and G1 proteins fall dramatically, and the cells accumulate in the G1 phase of the cell cycle, leading to almost complete proliferation arrest. Our results show that, compared with IMP-1 and IMP-2, IMP-3 is enriched in the nucleus, where it binds the transcripts of CCND1, D3 and G1. The nuclear localization of IMP-3 depends on its protein partner HNRNPM and is indispensable for the post-transcriptional regulation of expression of the cyclins. Cytoplasmic retention of IMP-3 and HNRNPM in human cancer cells leads to significant drop in proliferation. In conclusion, a nuclear IMP-3-HNRNPM complex is important for the efficient synthesis of CCND1, D3 and G1 and for the proliferation of human cancer cells.

  14. Feasibility of one-eighth time gated myocardial perfusion SPECT functional imaging using IQ-SPECT

    Energy Technology Data Exchange (ETDEWEB)

    Caobelli, Federico; Thackeray, James T.; Bengel, Frank M. [Medizinische Hochschule Hannover, Klinik fuer Nuklearmedizin, Hannover (Germany); Soffientini, Alberto; Pizzocaro, Claudio; Guerra, Ugo Paolo [Fondazione Poliambulanza, Department of Nuclear Medicine, Brescia (Italy)

    2015-11-15

    IQ-SPECT, an add-on to general purpose cameras based on multifocal collimation, can reduce myocardial perfusion imaging (MPI) acquisition times to one-fourth that of standard procedures (to 12 s/view). In a phantom study, a reduction of the acquisition time to one-eighth of the standard time (to 6 s/view) was demonstrated as feasible. It remains unclear whether such a reduction could be extended to clinical practice. Fifty patients with suspected or diagnosed CAD underwent a 2-day stress-rest {sup 99m}Tc-sestamibi MPI protocol. Two consecutive SPECT acquisitions (6 and 12 s/view) were performed. Electrocardiogram-gated images were reconstructed with and without attenuation correction (AC). Polar maps were generated and visually scored by two blinded observers for image quality and perfusion in 17 segments. Global and regional summed stress score (SSS), summed rest score (SRS) and summed difference score (SDS) were determined. Left ventricular volumes and ejection fraction were calculated based on automated contour detection. Image quality was scored higher with the 12 s/view acquisition, both with and without AC. Summed scores were statistically comparable between the 6 s/view and the 12 s/view acquisition, both globally and in individual coronary territories (e.g. in images with AC, SSS were 6.6 ± 8.3 and 6.2 ± 8.2 with 6 s and 12 s/view, respectively, p = 0.10; SRS were 3.9 ± 5.6 and 3.5 ± 5.3, respectively, p = 0.19; and SDS were 2.8 ± 5.7 and 2.6 ± 5.7, respectively, p = 0.59). Both acquisitions allowed MPI-based diagnosis of CAD in 25 of the 50 patients (with AC). Calculated end-diastolic volume (EDV) and end-systolic volume (ESV) were modestly higher with the 6 s/view acquisition than with the 12 s/view acquisition (EDV +4.8 ml at rest and +3.7 ml after stress, p = 0.003; ESV +4.1 ml at rest and +2.6 ml after stress, p = 0.01), whereas the ejection fraction did not differ (-1.2 % at rest, p = 0.20, and -0.9 % after stress, p = 0.27). Image quality and

  15. SPECT and PET in Late-Life Depression

    NARCIS (Netherlands)

    Vieira, Tiago S; Oude Voshaar, Richard; De Deyn, Peter; Dierckx, Rudi; van Waarde, Aren; Dierckx, Rudi AJO; Otte, Andreas; de Vries, Erik FJ; van Waarde, Aren; den Boer, Johan A

    2014-01-01

    Late-life late onset depression (i.e., depression with an age of onset above 60 yrs) appears to differ from depression with early onset in its association with cerebral small vessel disease, beta-amyloid and tau deposition, and neurodegenerative processes. Multimodality imaging (SPECT, PET, MRI) sup

  16. CALOR87: HETC87, MICAP, EGS4, and SPECT

    Energy Technology Data Exchange (ETDEWEB)

    Gabriel, T.A.; Alsmiller, F.S.; Alsmiller, R.G. Jr.; Bishop, B.L.; Hermann, O.W.; Johnson, J.O.

    1987-01-01

    A brief history of CALOR (HETC, EGS, MICAP, SPECT) is presented to indicate the evolution of this code system. Details concerning the current modifications and additions to the high-energy transport code, HETC, are also presented and new comparisons with experimental data are included to verify the new physics improvements. 27 refs., 5 figs.

  17. Small hepatocellular carcinomas in chronic liver disease: Detection with SPECT

    Energy Technology Data Exchange (ETDEWEB)

    Kudo, M.; Hirasa, M.; Takakuwa, H.; Ibuki, Y.; Fujimi, K.; Miyamura, M.; Tomita, S.; Komori, H.; Todo, A.; Kitaura, Y.

    1986-06-01

    Single-photon emission computed tomography (SPECT) performed using a rotating gamma camera was compared with ..cap alpha../sub 1/-fetoprotein (AFP) assay, conventional liver scintigraphy, ultrasound (US) imaging, computed tomography (CT), and selective celiac angiography in 40 patients with a total of 50 small hepatocellular carcinomas (HCCs;<5 cm). The detection rates of US and CT were determined on an initial screening study and on a second, more precisely focused study. The detection rate of small HCCs by the various modalities was as follows: AFP, 13%; liver scintigraphy, 36%; SPECT, 72%; initial screening US, 80%; second, more precise US studies, 94%; initial screening CT, 64%; second, more precise CT study, 82%; angiography, 88%. Although SPECT was inferior to the initial screening US examination in detecting HCCs less than 2 cm in size, its sensitivity was identical to that of the initial screening US study for detecting HCCs of 2-5 cm. The combination of SPECT and US was an excellent method for the early detection of HCCs, yielding a detection rate of 94%.

  18. Role of SPECT/CT in diagnosis of hepatic hemangiomas

    Institute of Scientific and Technical Information of China (English)

    Jian-Guo Zheng; Zhi-Ming Yao; Chong-Ye Shu; Ying Zhang; Xia Zhang

    2005-01-01

    AIM: To investigate the role of SPECT/CT in the diagnosis of hepatic hemangiomas whose anatomical positions are not ideal, situated adjacent to the heart, the inferior cava,hepatic vessels or abdominal aorta, etc.METHODS: The hepatic perfusion, blood pool, and fusion imaging were carried out using SPECT/CT in 54 patients,who were suspected for hepatic hemangiomas. When the anatomical positions were not ideal, the diagnosis was difficult by SPECT only. So the information of computed tomography (CT) was applied to help in diagnosing. The results were recorded as hemangiomas or not.RESULTS: Of the 54 patients, 31 patients were diagnosed as suffering from hepatic hemangiomas. The anatomical positions of eight patients' hepatic hemangiomas (25.81%)were not ideal. Among these lesions of the eight patients,three patients' hepatic lesions were located near to the abdominal aorta, one to the heart, and four to the inferior cava. In addition, six abnormal radioactivity accumulation regions, adjacent to the heart and inferior cava, with the help of CT, were confirmed to be the imaging of inferior cava other than hepatic hemangiomas.CONCLUSION: When the anatomical positions of hepatic hemangiomas are not good enough for diagnosis, the fusion imaging of SPECT/CT is a simple and efficient method for differential diagnosis.

  19. Cerebral fat embolism studied with MRI and SPECT

    Energy Technology Data Exchange (ETDEWEB)

    Erdem, E. (Dept. of Neurology, Hacettepe Univ. School of Medicine, Ankara (Turkey)); Namer, I.J. (Inst. of Biophysics, Faculty of Medicine, Louis Pasteur Univ., Strasbourg (France)); Saribas, O. (Dept. of Neurology, Hacettepe Univ. School of Medicine, Ankara (Turkey)); Aras, T. (Dept. of Nuclear Medicine, Hacettepe Univ. School of Medicine Ankara (Turkey)); Tan, E. (Dept. of Neurology, Hacettepe Univ. School of Medicine, Ankara (Turkey)); Bekdik, C. (Dept. of Nuclear Medicine, Hacettepe Univ. School of Medicine Ankara (Turkey)); Zileli, T. (Dept. of Neurology, Hacettepe Univ. School of Medicine, Ankara (Turkey))

    1993-03-01

    In a patient with fat embolism to the brain CT showed no abnormality. MRI performed after recovery from coma, when the patient had aphasia and quadriparesis, demonstrated multiple high signal abnormalities in the white matter on both T1- and T2-weighted images. HMPAO-SPECT showed left-sided hypoperfusion which resolved in parallel with clinical improvement 1 month later. (orig.)

  20. Molecular Imaging of Conscious, Unrestrained Mice with AwakeSPECT

    Energy Technology Data Exchange (ETDEWEB)

    Baba, Justin S. [Oak Ridge National Laboratory; Endres, Christopher J. [Johns Hopkins, Baltimore; Foss, Catherine A. [Johns Hopkins, Baltimore; Nimmagadda, Sridhar [Johns Hopkins, Baltimore; Jung, Hyeyun [Johns Hopkins, Baltimore; Goddard, James S. [Oak Ridge National Laboratory; Lee, Seung Joon [JLAB; McKisson, John [JLAB; Smith, Mark F. [University of Maryland; Stolin, Alexander V. [West Virginia University; Weisenberger, Andrew G. [JLAB; Pomper, Martin G. [Johns Hopkins, Baltimore

    2013-06-01

    We have developed a SPECT imaging system, AwakeSPECT, to enable molecular brain imaging of untrained mice that are conscious, unanesthetized, and unrestrained. We accomplished this with head tracking and motion correction techniques. Methods: The capability of the system for motion-corrected imaging was demonstrated with a ^99mTc-pertechnetate phantom, ^99mTc-methylene diphosphonate bone imaging, and measurement of the binding potential of the dopamine transporter radioligand ^123I-ioflupane in mouse brain in the awake and anesthetized (isoflurane) states. Stress induced by imaging in the awake state was assessed through measurement of plasma corticosterone levels. Results: AwakeSPECT provided high-resolution bone images reminiscent of those obtained from CT. The binding potential of ^123I-ioflupane in the awake state was on the order of 50% of that obtained with the animal under anesthesia, consistent with previous studies in nonhuman primates. Levels of stress induced were on the order of those seen in other behavioral tasks and imaging studies of awake animals. Conclusion: These results demonstrate the feasibility of SPECT molecular brain imaging of mice in the conscious, unrestrained state and demonstrate the effects of isoflurane anesthesia on radiotracer uptake.

  1. Molecular Imaging of Conscious, Unrestrained Mice with AwakeSPECT

    Energy Technology Data Exchange (ETDEWEB)

    Baba, Justin S [ORNL; Endres, Christopher [Johns Hopkins University; Foss, Catherine [Johns Hopkins University; Nimmagadda, Sridhar [Johns Hopkins University; Jung, Hyeyun [Johns Hopkins University; Goddard Jr, James Samuel [ORNL; Lee, Seung Joon [Jefferson Lab; McKisson, John [Jefferson Lab; Smith, Mark F. [University of Maryland School of Medicine, The, Baltimore, MD; Stolin, Alexander [West Virginia University, Morgantown; Weisenberger, Andrew G. [Jefferson Lab; Pomper, Martin [Johns Hopkins University

    2013-01-01

    We have developed a SPECT imaging system, AwakeSPECT, to enable molecular brain imaging of untrained mice that are conscious, unanesthetized, and unrestrained. We accomplished this with head tracking and motion correction techniques. Methods: The capability of the system for motion-corrected imaging was demonstrated with a 99mTc-pertechnetate phantom, 99mTcmethylene diphosphonate bone imaging, and measurement of the binding potential of the dopamine transporter radioligand 123I-ioflupane in mouse brain in the awake and anesthetized (isoflurane) states. Stress induced by imaging in the awake state was assessed through measurement of plasma corticosterone levels. Results: AwakeSPECT provided high-resolution bone images reminiscent of those obtained from CT. The binding potential of 123I-ioflupane in the awake state was on the order of 50% of that obtained with the animal under anesthesia, consistent with previous studies in nonhuman primates. Levels of stress induced were on the order of those seen in other behavioral tasks and imaging studies of awake animals. Conclusion: These results demonstrate the feasibility of SPECT molecular brain imaging of mice in the conscious, unrestrained state and demonstrate the effects of isoflurane anesthesia on radiotracer uptake.

  2. Diagnosis of lung embolism by V / Q scintigraphy. Pt. 2. SPECT and SPECT-CT for pulmonary diagnostic in nuclear medicine; V / Q-Szintigrafie zur Diagnostik der Lungenembolie. T. 2. SPECT und SPECT-CT in der nuklearmedizinischen Lungendiagnostik

    Energy Technology Data Exchange (ETDEWEB)

    Krause, T. [Universitaetsklinik fuer Nuklearmedizin, Bern (Switzerland). Inselspital

    2008-12-15

    Imaging of the pulmonary ventilation and perfusion by using SPECT proved to be a useful development of this examination. At first this article gives a brief overview of the technical aspects followed by a discussion of clinical questions regarding pulmonary embolism, preoperative assessment of postoperative lung function as well as radioaerosol deposition and clearance. (orig.)

  3. Thallium-201 accumulation in cerebral candidiasis: Unexpected finding on SPECT

    Energy Technology Data Exchange (ETDEWEB)

    Tonami, N.; Matsuda, H.; Ooba, H.; Yokoyama, K.; Hisada, K.; Ikeda, K.; Yamashita, J. (Kanazawa Univ. (Japan))

    1990-06-01

    The authors present an unexpected finding of Tl-201 uptake in the intracerebral lesions due to candidiasis. SPECT demonstrated the extent of the lesions and a high target-to-background ratio. The regions where abnormal Tl-201 accumulation was seen were nearly consistent with CT scans of those enhanced by a contrast agent. After treatment, most of the abnormal Tl-201 accumulation disappeared.

  4. Accelerated Monte Carlo simulation for scatter correction in SPECT

    NARCIS (Netherlands)

    Jong, Hugo Wilhelmus Antonius Maria de

    2002-01-01

    Single Photon Emission Tomography (SPECT) is often used in the clinical practice to image the distribution of photon-emitting pharmaceuticals in the patient. From this distribution, functional information can be obtained (e.g. perfusion and metabolic processes). To assess the viability of myocardia

  5. Reduced contralateral hemispheric flow measured by SPECT in cerebellar lesions

    DEFF Research Database (Denmark)

    Sönmezoğlu, K; Sperling, B; Henriksen, T

    1993-01-01

    to the cerebellar lesion CBF showed variable alterations. A relative CBF decrease was seen in upper part of basal ganglia in all four cases, but it was not a constant phenomenon. A relative CBF increase in both early and late SPECT scans was seen at low levels of neostriatum in two cases. The remote CBF changes...

  6. Imp2, the PSTPIP homolog in fission yeast, affects sensitivity to the immunosuppressant FK506 and membrane trafficking in fission yeast

    Energy Technology Data Exchange (ETDEWEB)

    Kita, Ayako; Higa, Mari [Laboratory of Molecular Pharmacogenomics, School of Pharmaceutical Sciences, Kinki University, 3-4-1 Kowakae, Higashi-Osaka 577-8502 (Japan); Doi, Akira [Laboratory of Molecular Pharmacogenomics, School of Pharmaceutical Sciences, Kinki University, 3-4-1 Kowakae, Higashi-Osaka 577-8502 (Japan); Japan Society for the Promotion of Science, 1-8 Chiyoda-ku, Tokyo 102-8472 (Japan); Satoh, Ryosuke [Laboratory of Molecular Pharmacogenomics, School of Pharmaceutical Sciences, Kinki University, 3-4-1 Kowakae, Higashi-Osaka 577-8502 (Japan); Sugiura, Reiko, E-mail: sugiurar@phar.kindai.ac.jp [Laboratory of Molecular Pharmacogenomics, School of Pharmaceutical Sciences, Kinki University, 3-4-1 Kowakae, Higashi-Osaka 577-8502 (Japan)

    2015-02-13

    Cytokinesis is a highly ordered process that divides one cell into two cells, which is functionally linked to the dynamic remodeling of the plasma membrane coordinately with various events such as membrane trafficking. Calcineurin is a highly conserved serine/threonine protein phosphatase, which regulates multiple biological functions, such as membrane trafficking and cytokinesis. Here, we isolated imp2-c3, a mutant allele of the imp2{sup +} gene, encoding a homolog of the mouse PSTPIP1 (proline-serine-threonine phosphatase interacting protein 1), using a genetic screen for mutations that are synthetically lethal with calcineurin deletion in fission yeast. The imp2-c3 mutants showed a defect in cytokinesis with multi-septated phenotypes, which was further enhanced upon treatment with the calcineurin inhibitor FK506. Notably, electron micrographs revealed that the imp2-c3 mutant cells accumulated aberrant multi-lamella Golgi structures and putative post-Golgi secretory vesicles, and exhibited fragmented vacuoles in addition to thickened septa. Consistently, imp2-c3 mutants showed a reduced secretion of acid phosphatase and defects in vacuole fusion. The imp2-c3 mutant cells exhibited a weakened cell wall, similar to the membrane trafficking mutants identified in the same genetic screen such as ypt3-i5. These findings implicate the PSTPIP1 homolog Imp2 in Golgi/vacuole function, thereby affecting various cellular processes, including cytokinesis and cell integrity. - Highlights: • We isolated imp2-c3, in a synthetic lethal screen with calcineurin in fission yeast. • The imp2{sup +} gene encodes a component of the actin contractile ring similar to Cdc15. • The imp2-c3 mutants showed defects in cytokinesis, which were exacerbated by FK506. • The imp2-c3 mutants were defective in membrane trafficking and cell wall integrity. • Our study revealed a novel role for Imp2 in the Golgi/vacuolar membrane trafficking.

  7. The need of appropriate brain SPECT templates for SPM comparisons

    Energy Technology Data Exchange (ETDEWEB)

    Morbelli, S.; Altrinetti, V.; Piccardo, A. [S Martino Univ Hosp, Nucl MedUnit DIMI, I-16132 Genoa (Italy); Rodriguez, G.; Brugnolo, A.; Nobili, F. [S Martino Univ Hosp, Clin Neurophysiol Unit DiSEM, DiTeC, I-16132 Genoa (Italy); Mignone, A. [Osped Riuniti Bergamo, Nucl Med Unit, I-24100 Bergamo (Italy); Pupi, A. [Univ Florence, Nucl Med Unit DFP, Florence (Italy); Koulibaly, P.M. [CEA-Univ Nice Sophia Antipolis, TIRO, Dept Nucl Med, Ctr Antoine Lacassagne, Nice (France)

    2008-07-01

    Statistical parametric mapping (SPM) is used worldwide to compare brain perfusion single photon emission computed tomography (SPECT) data. The default template within the SPM package used for SPECT image normalization includes images of a group of healthy subjects studied with {sup 99m}TcHMPAO. Since [{sup 99m}Tc] HMPAO and [{sup 99m}Tc] ECD have shown to distribute differently in SPECT studies, we formulated the hypothesis that comparing set of [{sup 99m}Tc]ECD data normalized by means of a [{sup 99m}Tc]HMPAO template may lead to incorrect results. A customized [{sup 99m}Tc]ECD template was built with SPECT and magnetic resonance imaging (MRI) images of 22 neurologically healthy women. Then, two sets of subjects, i.e. a group of patients with very early Alzheimer's disease (eAD) and a matched control group, studied by means of [{sup 99m}Tc]ECD SPECT, were chosen for comparisons. The same statistical approach (t-test between eAD patients and controls and correlation analysis between brain SPECT and a cognitive score) was applied twice, i.e. after normalization with either the default [{sup 99m}Tc]HMPAO template or the customized [{sup 99m}Tc]ECD template. In the comparison between eAD and controls, a cluster of difference in the posterior-cingulate gyrus of both hemispheres was only highlighted when using the customized [{sup 99m}Tc]ECD template, but was missed when using the default [{sup 99m}Tc]HMPAO template. In the correlation between brain perfusion and a cognitive score, the significant cluster was more significant and far more extended, also including the right superior temporal gyrus, using the customized [{sup 99m}Tc]ECD template than using the default [{sup 99m}Tc]HMPAO template. These data suggest the need of customized, radiopharmaceutical-matched SPECT templates to be used within the SPM package. The present customized [{sup 99m}Tc]ECD template is now freely available on the web. (authors)

  8. [Variables that influence the indication of a second myocardial perfusion gated-SPECT after a normal stress-rest gated SPECT].

    Science.gov (United States)

    Romero-Farina, G; Candell-Riera, J; Aguadé-Bruix, S; Cuberas-Borrós, G; Pizzi, M N; Santos, A; de León, G; García-Dorado, D

    2014-01-01

    The objective of this study was to investigate predictor variables at the moment of normal stress-rest myocardial perfusion gated SPECT for indication of a second gated SPECT. A prospective, single center cohort study was conducted. We evaluated 2326 consecutive patients (age 63.6 ± 13 years, 57.3% females) without perfusion defects and with normal left ventricular ejection fraction on a myocardial perfusion gated SPECT. Clinical and stress test variables were studied to predict indication of a second gated SPECT and presence of reversible perfusion defects in the second gated SPECT. During a mean follow-up of 3.6 ± 2 years a second gated SPECT was performed in 286 patients (12.3%). Independent predictor variables of a second gated SPECT were presence of three or more cardiovascular risk factors (χ(2): 5.510; HR: 1.4; p=0.019), previous acute myocardial infarction (χ(2): 3.867; HR: 1.4; p=0.049), previous coronary revascularization (χ(2): 41.081; HR: 2.5; p<0.001), and a positive stress test (χ(2): 8.713; HR: 1.5; p=0.003). Observation of perfusion defects in the 280 patients in whom a second stress-rest gated SPECT was performed was more likely in male patients (χ(2): 4.322; HR: 1.9; p=0.038) who had a first pure pharmacological gated-SPECT (χ(2): 7.182; HR: 2.6; p=0.007). In patients with a first normal myocardial perfusion gated SPECT, various clinical factors and variables derived from the stress test affect the indication of a second gated SPECT and the presence of ischemia in the latter. Copyright © 2013 Elsevier España, S.L. and SEMNIM. All rights reserved.

  9. Collimator design for a multipinhole brain SPECT insert for MRI

    Energy Technology Data Exchange (ETDEWEB)

    Van Audenhaege, Karen; Van Holen, Roel; Vanhove, Christian; Vandenberghe, Stefaan [Department of Electronics and Information Systems, Ghent University-iMinds Medical IT, MEDISIP-IBiTech, De Pintelaan 185 block B/5, Ghent B-9000 (Belgium)

    2015-11-15

    Purpose: Brain single photon emission computed tomography (SPECT) imaging is an important clinical tool, with unique tracers for studying neurological diseases. Nowadays, most commercial SPECT systems are combined with x-ray computed tomography (CT) in so-called SPECT/CT systems to obtain an anatomical background for the functional information. However, while CT images have a high spatial resolution, they have a low soft-tissue contrast, which is an important disadvantage for brain imaging. Magnetic resonance imaging (MRI), on the other hand, has a very high soft-tissue contrast and does not involve extra ionizing radiation. Therefore, the authors designed a brain SPECT insert that can operate inside a clinical MRI. Methods: The authors designed and simulated a compact stationary multipinhole SPECT insert based on digital silicon photomultiplier detector modules, which have shown to be MR-compatible and have an excellent intrinsic resolution (0.5 mm) when combined with a monolithic 2 mm thick LYSO crystal. First, the authors optimized the different parameters of the SPECT system to maximize sensitivity for a given target resolution of 7.2 mm in the center of the field-of-view, given the spatial constraints of the MR system. Second, the authors performed noiseless simulations of two multipinhole configurations to evaluate sampling and reconstructed resolution. Finally, the authors performed Monte Carlo simulations and compared the SPECT insert with a clinical system with ultrahigh-resolution (UHR) fan beam collimators, based on contrast-to-noise ratio and a visual comparison of a Hoffman phantom with a 9 mm cold lesion. Results: The optimization resulted in a stationary multipinhole system with a collimator radius of 150.2 mm and a detector radius of 172.67 mm, which corresponds to four rings of 34 diSPM detector modules. This allows the authors to include eight rings of 24 pinholes, which results in a system volume sensitivity of 395 cps/MBq. Noiseless simulations

  10. Collimator design for a multipinhole brain SPECT insert for MRI.

    Science.gov (United States)

    Van Audenhaege, Karen; Van Holen, Roel; Vanhove, Christian; Vandenberghe, Stefaan

    2015-11-01

    Brain single photon emission computed tomography (SPECT) imaging is an important clinical tool, with unique tracers for studying neurological diseases. Nowadays, most commercial SPECT systems are combined with x-ray computed tomography (CT) in so-called SPECT/CT systems to obtain an anatomical background for the functional information. However, while CT images have a high spatial resolution, they have a low soft-tissue contrast, which is an important disadvantage for brain imaging. Magnetic resonance imaging (MRI), on the other hand, has a very high soft-tissue contrast and does not involve extra ionizing radiation. Therefore, the authors designed a brain SPECT insert that can operate inside a clinical MRI. The authors designed and simulated a compact stationary multipinhole SPECT insert based on digital silicon photomultiplier detector modules, which have shown to be MR-compatible and have an excellent intrinsic resolution (0.5 mm) when combined with a monolithic 2 mm thick LYSO crystal. First, the authors optimized the different parameters of the SPECT system to maximize sensitivity for a given target resolution of 7.2 mm in the center of the field-of-view, given the spatial constraints of the MR system. Second, the authors performed noiseless simulations of two multipinhole configurations to evaluate sampling and reconstructed resolution. Finally, the authors performed Monte Carlo simulations and compared the SPECT insert with a clinical system with ultrahigh-resolution (UHR) fan beam collimators, based on contrast-to-noise ratio and a visual comparison of a Hoffman phantom with a 9 mm cold lesion. The optimization resulted in a stationary multipinhole system with a collimator radius of 150.2 mm and a detector radius of 172.67 mm, which corresponds to four rings of 34 diSPM detector modules. This allows the authors to include eight rings of 24 pinholes, which results in a system volume sensitivity of 395 cps/MBq. Noiseless simulations show sufficient axial

  11. Scatter and attenuation correction in Technetium-99m brain SPECT

    Energy Technology Data Exchange (ETDEWEB)

    Hashimoto, Jun [Keio Univ., Tokyo (Japan). School of Medicine

    1997-11-01

    The imaging in the nuclear medicine is inferior to other modalities in the spatial resolution, but it can quantitatively evaluate the biological function. The attempt to measure the absolute or the relative values of local functions quantitatively based on the SPECT of the brain or the heart, has become popular recently. It is not only necessary to introduce an appropriate analytical model for each radioisotope tracer for the correct quantification, but also to be equipped with the photographing system to measure correctly the accumulation of the tracer. The main cause to decrease the precision of the photographing system is the scatter and the absorption (attenuation) of photon. In the present study we attempted to enhance the quantification of the brain SPECT by the usable and practical correction method for the scatter and the attenuation in the daily clinical business. We used the triple-energy-window (TEW) method as the scatter correction and the attenuation calculation map obtained from the transmission scanning (TCT) by the external source as the attenuation correction. We examined the brain SPECT in combination with these two correction methods. We could not measure correctly the amount of the tracer accumulation by only one correction method, and obtain the excellent quantitative SPECT images in combination with both correction methods. We could get excellently stable data by collecting the emission and the transmission data simultaneously as well as separately, and the excellent images by the arithmetical treatment of data between the scatter beam and the detector correction when we used {sup 99m}Tc as the source which is economically cheap, and adopted the combination of the tracers labeled by the same kind of radioisotopes. Altogether we expect that our method is very practical and will contribute to enhance the accuracy in quantitative analysis of clinical brain SPECT imaging. (K.H.)

  12. SPECT in der Diagnostik von Parkinson-Syndromen

    Directory of Open Access Journals (Sweden)

    Pirker W

    2004-01-01

    Full Text Available Nur die Hälfte der Patienten mit einem in der Allgemeinpraxis diagnostizierten Morbus Parkinson erfüllt in der neurologischen Untersuchung tatsächlich die klinisch-diagnostischen Kriterien der Erkrankung. Post-mortem-Untersuchungen haben gezeigt, daß die Treffsicherheit für die klinische Diagnose eines M. Parkinson selbst in spezialisierten Zentren und unter Einhaltung strikter Kriterien bei maximal 90 % liegen dürfte. Der Essentielle Tremor und andere Tremorsyndrome, medikamenteninduzierte Parkinson-Syndrome, vaskuläre Parkinson-Syndrome, Parkinson-Syndrome im Rahmen von Demenzerkrankungen und atypische Parkinson-Erkrankungen degenerativer Ätiologie wie die Multisystematrophie führen am häufigsten zu Fehldiagnosen. SPECT-Untersuchungen mit Dopamintransporter-Liganden wie [123 I] beta-CIT erlauben bereits im Frühstadium eine zuverlässige Dokumentation der dopaminergen Degeneration beim M. Parkinson und atypischen Parkinson-Erkrankungen. Damit ist eine zuverlässige Abgrenzung dieser Erkrankungen von nichtdegenerativen Parkinson- und Tremorsyndromen möglich. Die Dopamintransporter-SPECT erlaubt darüber hinaus eine Differenzierung der Demenz mit Lewy-Körperchen von der Alzheimer-Demenz. SPECT-Untersuchungen postsynaptischer Dopamin-D2-Rezeptoren mit [123 I]IBZM können zur Differentialdiagnose zwischen M. Parkinson und Multisystematrophie bzw. der Progressiven Supranukleären Paralyse beitragen. In der folgenden Übersicht werden typische SPECT-Befunde bei Parkinson-Syndromen und klinische Indikationen für SPECT-Untersuchungen bei diesen Störungen dargestellt.

  13. Advanced reconstruction of attenuation maps using SPECT emission data only

    Science.gov (United States)

    Salomon, André; Goedicke, Andreas; Aach, Til

    2009-02-01

    Today, attenuation corrected SPECT, typically performed using CT or Gadolinium line source based transmission scans, is more and more becoming standard in many medical applications. Moreover, the information about the material density distribution provided by these scans is key for other artifact compensation approaches in advanced SPECT reconstruction. Major drawbacks of these approaches are the additional patient radiation and hardware/maintenance costs as well as the additional workflow effort, e.g. if the CT scans are not performed on a hybrid scanner. It has been investigated in the past, whether it is possible to recover this structural information solely from the SPECT scan data. However, the investigated methods often result in noticeable image artifacts due to cross-dependences between attenuation and activity distribution estimation. With the simultaneous reconstruction method presented in this paper, we aim to effectively prevent these typical cross-talk artifacts using a-priori known atlas information of a human body. At first, an initial 3D shape model is coarsely registered to the SPECT data using anatomical landmarks and each organ structure within the model is identified with its typical attenuation coefficient. During the iterative reconstruction based on a modified ML-EM scheme, the algorithm simultaneously adapts both, the local activity estimation and the 3D shape model in order to improve the overall consistency between measured and estimated sinogram data. By explicitly avoiding topology modifications resulting in a non-anatomical state, we ensure that the estimated attenuation map remains realistic. Several tests with simulated as well as real patient SPECT data were performed to test the proposed algorithm, which demonstrated reliable convergence behaviour in both cases. Comparing the achieved results with available reference data, an overall good agreement for both cold as well as hot activity regions could be observed (mean deviation: -5.98%).

  14. Update: SPECT in der Diagnostik von Parkinson-Syndromen

    Directory of Open Access Journals (Sweden)

    Pirker W

    2015-01-01

    Full Text Available Die Diagnose des Morbus Parkinson fußt primär auf Anamnese und klinischer Untersuchung. Die Abgrenzung von milden Bewegungsstörungen bei ansonsten gesunden älteren Personen, von medikamenteninduzierten und vaskulären Parkinson-Syndromen sowie von Tremor-Syndromen kann jedoch schwierig sein. Die Differenzierung zwischen M. Parkinson und atypischen Parkinson-Erkrankungen wie der Multisystematrophie (MSA und der progressiven supranukleären Paralyse (PSP ist im Frühstadium häufig unmöglich und kann auch in fortgeschrittenen Stadien Probleme bereiten. Die SPECT-Untersuchung mit Dopamin-Transporter- (DAT- Liganden wie 123I-FP-CIT erlaubt mit wenigen Ausnahmen bereits im Frühstadium eine Dokumentation der dopaminergen Degeneration beim M. Parkinson und atypischen Parkinson- Erkrankungen und damit meist eine Abgrenzung von nichtdegenerativen Parkinson- und Tremor-Syndromen. Sie kann die einzelnen degenerativen Parkinson-Syndrome jedoch nicht voneinander unterscheiden. Die Untersuchung postsynaptischer Dopamin- D2-Rezeptoren mit der 123I-IBZM-SPECT kann zur Differenzierung von M. Parkinson und MSA bzw. PSP beitragen. Neueren Untersuchungen zufolge dürften die Untersuchung des zerebralen Glukosemetabolismus mittels 18F-FDG-PET sowie moderne MRT-basierte Methoden in dieser Fragestellung jedoch eine höhere Sensitivität und Spezifität haben. Während Medikamente die diagnostische Aussagekraft von DAT-SPECT-Untersuchungen selten beeinflussen, kann das Ergebnis der IBZM-SPECT sowohl durch dopaminerge Substanzen als auch durch Dopaminantagonisten stark verfälscht werden. Die richtige Interpretation von SPECT-Daten setzt darüber hinaus die Kenntnis der individuellen strukturellen bildgebenden Befunde voraus. Eine MRT- oder CCTUntersuchung sollte zum Ausschluss von läsionellen Parkinson-Syndromen und zur Dokumentation von Kopathologien immer Bestandteil der Parkinson-Abklärung sein.

  15. A Carbapenem-Resistant Pseudomonas aeruginosa Isolate Harboring Two Copies of blaIMP-34 Encoding a Metallo-β-Lactamase.

    Directory of Open Access Journals (Sweden)

    Tatsuya Tada

    Full Text Available A carbapenem-resistant strain of Pseudomonas aeruginosa, NCGM1984, was isolated in 2012 from a hospitalized patient in Japan. Immunochromatographic assay showed that the isolate was positive for IMP-type metallo-β-lactamase. Complete genome sequencing revealed that NCGM1984 harbored two copies of blaIMP-34, located at different sites on the chromosome. Each blaIMP-34 was present in the same structures of the class 1 integrons, tnpA(ISPa7-intI1-qacG-blaIMP-34-aac(6'-Ib-qacEdelta1-sul1-orf5-tniBdelta-tniA. The isolate belonged to multilocus sequence typing ST235, one of the international high-risk clones. IMP-34, with an amino acid substitution (Glu126Gly compared with IMP-1, hydrolyzed all β-lactamases tested except aztreonam, and its catalytic activities were similar to IMP-1. This is the first report of a clinical isolate of an IMP-34-producing P. aeruginosa harboring two copies of blaIMP-34 on its chromosome.

  16. Skeletal scintigraphy and SPECT/CT in orthopedic imaging; Knochenszintigrafie und SPECT/CT bei orthopaedischen Fragestellungen

    Energy Technology Data Exchange (ETDEWEB)

    Klaeser, B.; Walter, M.; Krause, T. [Inselspital Bern (Switzerland). Universitaetsklinik fuer Nuklearmedizin

    2011-03-15

    Multi-modality imaging with SPECT-CT in orthopaedics combines the excellent sensitivity of scintigraphy with the morphological information of CT as a key for specific interpretation of findings in bone scans. The result is an imaging modality with the clear potential to prove of value even in a competitive setting dominated by MRI, and to significantly add to diagnostic imaging in orthopaedics. SPECT-CT is of great value in the diagnostic evaluation after fractures, and - in contrast to MRI - it is well suited for imaging in patients with osteosyntheses and metallic implants. In sports medicine, SPECT-CT allows for a sensitive and specific detection of osseous stress reactions before morphological changes become detectable by CT or MRI. In patients with osseous pain syndromes, actively evolving degenerative changes as a cause of pain can be identified and accurately localized. Further, particularly prospective diagnostic studies providing comparative data are needed to strengthen the position of nuclear imaging in orthopaedics and sports medicine and to help implementing SPECT/CT in diagnostic algorithms. (orig.)

  17. Survival Outcomes and Tumor IMP3 Expression in Patients with Sarcomatoid Metastatic Renal Cell Carcinoma

    Directory of Open Access Journals (Sweden)

    Srinivas K. Tantravahi

    2015-01-01

    Full Text Available Metastatic renal cell carcinoma with sarcomatoid histology (SmRCC is associated with poor survival. No data is available from randomized trials on the efficacy of vascular endothelial growth factor (VEGF and mammalian target of rapamycin (mTOR inhibitors in SmRCC. We identified SmRCC patients from a single institutional database. To identify predictive and prognostic biomarkers, immunohistochemistry (IHC analysis was performed on the tumor samples for downstream targets of VEGF and mTOR pathways. Survival outcomes were stratified by IHC analysis, extent of sarcomatoid component, Memorial Sloan-Kettering Cancer Center (MSKCC, and Heng risk criteria. Twenty-seven patients with SmRCC were included. First line therapy included targeted therapy (n=19, immunotherapy (n=4, cytotoxic chemotherapy (n=1, and no treatment (n=3. Median OS was 8.2 months (95% CI 3.8–14.2 months. Median survival in months, based on MSKCC and Heng risk groups, was favorable 89.3 versus 84.5, intermediate 9.5 versus 12.7, and poor 3.9 versus 5.1. None of the IHC markers predicted outcomes of treatment with VEGF or mTOR inhibitors. Only tumor IMP3 expression was associated with inferior OS, although not statistically significant (IMP3 negative 14.2 versus IMP3 positive 4.9 months; HR 0.46, 95% CI 0.16–1.21; P=0.12. The study was limited by small sample size.

  18. Characterization of a novel Klebsiella pneumoniae sequence type 476 carrying both bla KPC-2 and bla IMP-4.

    Science.gov (United States)

    Wang, Y; Cao, W; Zhu, X; Chen, Z; Li, L; Zhang, B; Wang, B; Tian, L; Wang, F; Liu, C; Sun, Z

    2012-08-01

    Carbapenemase-producing Klebsiella pneumoniae has recently spread rapidly throughout China. In this study, we characterized a carbapenem-resistant K. pneumoniae isolate that produced both KPC-2 and IMP-4 type carbapenemases. A clinical isolate of K. pneumoniae, resistant to both meropenem and imipenem, was recovered from a urine sample. Antibiotic susceptibility was determined using the broth microdilution method and Etest (bioMérieux, France). Pulsed-field gel electrophoresis and multilocus sequence typing (MLST) were used for gene type analysis. bla (KPC) and the encoding genes of ESBLs and plasmid-mediated AmpC enzymes were polymerase chain reaction (PCR) amplified and sequenced. Plasmids were analyzed by transformation, enzyme restriction and Southern blot. PCR analysis revealed that the isolate was simultaneously carrying bla (KPC-2), bla (IMP-4), bla (TEM-1), and bla (OKP-B) genes. MLST assigned the isolate to a novel sequence type, ST476. bla (KPC-2)-harbouring plasmids of the isolate and comparative strains had similar EcoRI and HindIII restriction maps, while IMP-4-harbouring plasmids had variable HindIII restriction maps. Coexistence of bla (KPC-2) and bla (IMP-4) was probably due to bla (IMP-4)-harbouring plasmid transmission into KPC-2-producing K. pneumoniae (ST476). The concomitant presence of these genes is alarming and poses both therapeutic and infection control problems.

  19. IMP improves water-holding capacity, physical and sensory properties of heat-induced gels from porcine meat.

    Science.gov (United States)

    Nakamura, Yukinobu; Migita, Koshiro; Okitani, Akihiro; Matsuishi, Masanori

    2014-05-01

    Water-holding capacity (WHC) of heat-induced pork gels was examined. The heat-induced gels were obtained from meat homogenates prepared by adding nine volumes of 0.3-0.5 mol/L NaCl solutions containing 9-36 mmol/L disodium inosine-5'-monophosphate (IMP) or 9 mmol/L tetrapotassium pyrophosphate (KPP) to minced pork. IMP at 36 mmol/L enhanced the WHC to the same level as attained by KPP. Physical and sensory properties of heat-induced gels were also examined. The heat-induced gels were prepared from porcine meat homogenates containing 0.3 mol/L NaCl and 9-36 mmol/L IMP or 9 mmol/L KPP. IMP at 36 mmol/L enhanced the values of hardness, cohesiveness, gumminess and springiness, measured with a Tensipresser, and several organoleptic scores to the same level as the score attained by KPP. Thus, it is concluded that IMP is expected to be a practical substitute for pyrophosphates to improve the quality of sausages.

  20. Do império do olhar à arte de ver

    Directory of Open Access Journals (Sweden)

    JOÃO AUGUSTO FRAYZE-PEREIRA

    1995-10-01

    Full Text Available Martin Jay (1986 e John Rajchman (1988 escreveram ensaios sobre o espaço da visão e do visual na obra de Foucault. Porém, se a questão é a mesma, o modo de ver dos autores é oposto. Interrogando esses dois ensaios, este artigo pretende configurar uma outra visão sobre o império do olhar e a arte de ver nos escritos de Foucault. A relação entre visão e pensamento é considerada na perspectiva das problematizações.

  1. The network researchers' network: A social network analysis of the IMP Group 1985-2006

    DEFF Research Database (Denmark)

    Henneberg, Stephan C. M.; Ziang, Zhizhong; Naudé, Peter;

    The Industrial Marketing and Purchasing (IMP) Group is a network of academic researchers working in the area of business-to-business marketing. The group meets every year to discuss and exchange ideas, with a conference having been held every year since 1984 (there was no meeting in 1987...... components in some detail. The egonets of three of the original 'founding fathers' are examined in detail, and we draw comparisons as to how their publishing strategies vary. Finally, the paper draws some more general conclusions as to the insights that SNA can bring to those working within business-to-business...... marketing....

  2. A methodology for generating normal and pathological brain perfusion SPECT images for evaluation of MRI/SPECT fusion methods: application in epilepsy

    Science.gov (United States)

    Grova, C.; Jannin, P.; Biraben, A.; Buvat, I.; Benali, H.; Bernard, A. M.; Scarabin, J. M.; Gibaud, B.

    2003-12-01

    Quantitative evaluation of brain MRI/SPECT fusion methods for normal and in particular pathological datasets is difficult, due to the frequent lack of relevant ground truth. We propose a methodology to generate MRI and SPECT datasets dedicated to the evaluation of MRI/SPECT fusion methods and illustrate the method when dealing with ictal SPECT. The method consists in generating normal or pathological SPECT data perfectly aligned with a high-resolution 3D T1-weighted MRI using realistic Monte Carlo simulations that closely reproduce the response of a SPECT imaging system. Anatomical input data for the SPECT simulations are obtained from this 3D T1-weighted MRI, while functional input data result from an inter-individual analysis of anatomically standardized SPECT data. The method makes it possible to control the 'brain perfusion' function by proposing a theoretical model of brain perfusion from measurements performed on real SPECT images. Our method provides an absolute gold standard for assessing MRI/SPECT registration method accuracy since, by construction, the SPECT data are perfectly registered with the MRI data. The proposed methodology has been applied to create a theoretical model of normal brain perfusion and ictal brain perfusion characteristic of mesial temporal lobe epilepsy. To approach realistic and unbiased perfusion models, real SPECT data were corrected for uniform attenuation, scatter and partial volume effect. An anatomic standardization was used to account for anatomic variability between subjects. Realistic simulations of normal and ictal SPECT deduced from these perfusion models are presented. The comparison of real and simulated SPECT images showed relative differences in regional activity concentration of less than 20% in most anatomical structures, for both normal and ictal data, suggesting realistic models of perfusion distributions for evaluation purposes. Inter-hemispheric asymmetry coefficients measured on simulated data were found within

  3. A combined static-dynamic single-dose imaging protocol to compare quantitative dynamic SPECT with static conventional SPECT.

    Science.gov (United States)

    Sciammarella, Maria; Shrestha, Uttam M; Seo, Youngho; Gullberg, Grant T; Botvinick, Elias H

    2017-08-03

    SPECT myocardial perfusion imaging (MPI) is a clinical mainstay that is typically performed with static imaging protocols and visually or semi-quantitatively assessed for perfusion defects based upon the relative intensity of myocardial regions. Dynamic cardiac SPECT presents a new imaging technique based on time-varying information of radiotracer distribution, which permits the evaluation of regional myocardial blood flow (MBF) and coronary flow reserve (CFR). In this work, a preliminary feasibility study was conducted in a small patient sample designed to implement a unique combined static-dynamic single-dose one-day visit imaging protocol to compare quantitative dynamic SPECT with static conventional SPECT for improving the diagnosis of coronary artery disease (CAD). Fifteen patients (11 males, four females, mean age 71 ± 9 years) were enrolled for a combined dynamic and static SPECT (Infinia Hawkeye 4, GE Healthcare) imaging protocol with a single dose of (99m)Tc-tetrofosmin administered at rest and a single dose administered at stress in a one-day visit. Out of 15 patients, eleven had selective coronary angiography (SCA), 8 within 6 months and the rest within 24 months of SPECT imaging, without intervening symptoms or interventions. The extent and severity of perfusion defects in each myocardial region was graded visually. Dynamically acquired data were also used to estimate the MBF and CFR. Both visually graded images and estimated CFR were tested against SCA as a reference to evaluate the validity of the methods. Overall, conventional static SPECT was normal in ten patients and abnormal in five patients, dynamic SPECT was normal in 12 patients and abnormal in three patients, and CFR from dynamic SPECT was normal in nine patients and abnormal in six patients. Among those 11 patients with SCA, conventional SPECT was normal in 5, 3 with documented CAD on SCA with an overall accuracy of 64%, sensitivity of 40% and specificity of 83%. Dynamic SPECT image

  4. Uptake of SPECT radiopharmaceuticals in neocortical brain cultures

    Energy Technology Data Exchange (ETDEWEB)

    Jong, B.M. de; Royen, E.A. van

    1989-01-01

    The uptake, retention and uptake antagonism of /sup 201/Tl-DDC, /sup 201/Tl-Cl, /sup 123/I-IMP, /sup 99m/Tc-HMPAO and /sup 99m/Tc-O4/sup -/ were compared in rat neocortex cultures. /sup 201/Tl-DDC and /sup 123/I-IP revealed the highest uptake of radioactivity in the cultures. /sup 99m/Tc-HMPAO and /sup 123/I-IMP showed the highest retention of radioactivity within the tissue in washout experiments. Blocking of bioelectric activity by tetrodotoxin did not significantly affect the uptake of the radiopharmaceuticals (RPHA). Inhibition of Na K ATPase by ouabain inhibited the uptake of /sup 201/Tl-Cl (77%) and /sup 201/Tl-DDC (27%). Imipramine showed a significantly stronger inhibitory effect on /sup 123/I-IMP uptake in comparison with the effect on other RPHA. /sup 99m/Tc-O4/sup -/ was not concentrated within the cultured tissue. Under the in vitro conditions used in this study, the various RPHA were characterised by distinct differences in their interaction with cortical brain tissue.

  5. Optimization of labeling conditions of n-isopropyl-p-iodoamphetamine chloridate (IMP) with radioiodine. Biological distribution studies; Otimizacao das condicoes de marcacao do cloridrato de n-isopropil-p-iodoanfetamina (IMP) com radioiodo. Estudos de distribuicao biologica

    Energy Technology Data Exchange (ETDEWEB)

    Colturato, Maria Tereza

    2000-07-01

    The development of this work was based on a great interest from the medical community in the utilization of N-isopropyl-p-iodoamphetamine chloridate (IMP) labeled with {sup 123}l, for brain perfusion evaluation. The IMP was initially characterized by: Melting Point (MP), Infrared Spectrophotometry (IR), Nuclear Magnetic Resonance Spectrometry (NMR), Elemental Analysis and High Performance Liquid Chromatography (HPLC). After having chosen the ideal method (nucleophilic substitution) to label IMP with that used Cu(I) as reducing agent and ascorbic acid as catalyzing of Cu(II), studies were performed to optimize the labeling parameters of {sup 123}l-IMP: temperature reaction, time reaction, ascorbic acid mass, pH and molar ratio, and stability of the final product. The quality control method (ascending paper chromatographic) used to determine the radiochemistry purity showed to be efficient, fast and of easily handling for routine production. Biological distribution studies were performed with laboratory animals (mice) to determine the percent administered dose in the blood, different organs and whole body after intravenous administration of the radiopharmaceutical. Toxicological evaluation and in vitro study to determine the plasmatic protein binding were also done. The data of the biological distribution in mice have shown that the product crossed the intact blood brain barrier, for a enough time to obtain brain scintigraphic image, thus, allowing a follow up of further studies after the intravenous administration of the radiopharmaceutical. The {sup 123}l-IMP showed a blood clearance and then the principal elimination route was the urinary. The kinetic study of {sup 123}l-IMP, submitting blood samples data to BIEXP.BAS program, showed a biexponential pattern which allowed demonstrating that the compound presents a first phase of quick distribution and a second one slower corresponding to the equilibrium and elimination. Based on the results from radiochemical

  6. Receptor mapping in psychiatric patients with SPECT; Rezeptor-SPECT-Untersuchungen bei psychiatrischen Patienten

    Energy Technology Data Exchange (ETDEWEB)

    Schlegel, S. [Mainz Univ. (Germany). Psychiatrische Klinik

    1997-06-01

    This paper summarizes some data of our studies with the single-photon-emission-computerized tomography (SPECT), focussing on the dopamine-D2- and the benzodiazepine receptor mapping. Benzodiazepine receptors: Central benzodiazepine receptors (BZr) can be visualized with iomazenil which is an analogue of the benzodiazepine antagonist flumazenil, labeled with 123-iodine. Since the involvement of the BZr system is discussed in the pathogenesis of anxiety and depression, patients with these disorders were investigated. A third study investigated the BZr-occupancy during benzodiazepine treatment (lorazepam). Results: (a) Patients with panic disorders had lower iomazenil uptake values compared to epileptic patients. (b) Depressed patients showed a positive correlation between severity of illness and frontal uptake. (c) BZr occupancy during lorazepam treatment was measurable, but not associated with lorazepam plasma levels. Dopamine-D2-receptors: With 123-I-iodobenzamide (IBZM), and iodine-labeled dopamine receptor ligand, the D2 receptor density can be measured by a semiquantitative approach (striatum/frontal cortex=ST/FC). Therefore, we investigated the D2-receptor occupancy during treatment with typical and atypical neuroleptics in relationship to dosages (normalized with different formulas of chlorpromazine equivalents), side effects, and prolactin plasma levels. Results: Dependent on the selected formula for chlorpromazine equivalents, the ST/FC ratio was correlated with dosages. Side effects and prolactin plasma levels showed a negative association with lower ST/FC ratios. (orig.) [Deutsch] Der Beitrag soll einen Ueberblick ueber verschiedene eigene Studien geben, die die Darstellung von Dopamin-D2- und Benzodiazepinrezeptoren mit der Single-Photon-Emissions-Computertomographie (SPECT) untersuchten. Benzodiazepinrezeptoren: Jomazenil, der mit 123-Jod markierte Benzodiazepinantagonist Flumazenil, ermoeglicht die Darstellung der zentralen Benzodiazepinrezeptoren (BZr

  7. Cortical region of interest definition on SPECT brain images using X-ray CT registration

    Energy Technology Data Exchange (ETDEWEB)

    Tzourio, N.; Sutton, D. (Commissariat a l' Energie Atomique, Orsay (France). Service Hospitalier Frederic Joliot); Joliot, M. (Commissariat a l' Energie Atomique, Orsay (France). Service Hospitalier Frederic Joliot INSERM, Orsay (France)); Mazoyer, B.M. (Commissariat a l' Energie Atomique, Orsay (France). Service Hospitalier Frederic Joliot Antenne d' Information Medicale, C.H.U. Bichat, Paris (France)); Charlot, V. (Hopital Louis Mourier, Colombes (France). Service de Psychiatrie); Salamon, G. (CHU La Timone, Marseille (France). Service de Neuroradiologie)

    1992-11-01

    We present a method for brain single photon emission computed tomography (SPECT) analysis based on individual registration of anatomical (CT) and functional ([sup 133]Xe regional cerebral blood flow) images and on the definition of three-dimensional functional regions of interest. Registration of CT and SPECT is performed through adjustment of CT-defined cortex limits to the SPECT image. Regions are defined by sectioning a cortical ribbon on the CT images, copied over the SPECT images and pooled through slices to give 3D cortical regions of interest. The proposed method shows good intra- and interobserver reproducibility (regional intraclass correlation coefficient [approx equal]0.98), and good accuracy in terms of repositioning ([approx equal]3.5 mm) as compared to the SPECT image resolution (14 mm). The method should be particularly useful for analysing SPECT studies when variations in brain anatomy (normal or abnormal) must be accounted for. (orig.).

  8. Attenuation correction of myocardial SPECT images with X-ray CT. Effects of registration errors between X-ray CT and SPECT

    Energy Technology Data Exchange (ETDEWEB)

    Takahashi, Yasuyuki; Murase, Kenya [Osaka Univ., Suita (Japan). Graduate School of Medicine; Higashino, Hiroshi [Ehime Prefectural Imabari Hospital (Japan); Mochizuki, Teruhito [Ehime Univ., Matsuyama (Japan). School of Medicine; Motomura, Nobutoku [Toshiba Corp., Otawara, Tochigi (Japan). Medical Engineering Lab.

    2002-09-01

    Attenuation correction with an X-ray CT image is a new method to correct attenuation on SPECT imaging, but the effect of the registration errors between CT and SPECT images is unclear. In this study, we investigated the effects of the registration errors on myocardial SPECT, analyzing data from a phantom and a human volunteer. Registerion (fusion) of the X-ray CT and SPECT images was done with standard packaged software in three dimensional fashion, by using linked transaxial, coronal and sagittal images. In the phantom study, and X-ray CT image was shifted 1 to 3 pixels on the x, y and z axes, and rotated 6 degrees clockwise. Attenuation correction maps generated from each misaligned X-ray CT image were used to reconstruct misaligned SPECT images of the phantom filled with {sup 201}Tl. In a human volunteer, X-ray CT was acquired in different conditions (during inspiration vs. expiration). CT values were transferred to an attenuation constant by using straight lines; an attenuation constant of 0/cm in the air (CT value=-1,000 HU) and that of 0.150/cm in water (CT value=0 HU). For comparison, attenuation correction with transmission CT (TCT) data and an external {gamma}-ray source ({sup 99m}Tc) was also applied to reconstruct SPECT images. Simulated breast attenuation with a breast attachment, and inferior wall attenuation were properly corrected by means of the attenuation correction map generated from X-ray CT. As pixel shift increased, deviation of the SPECT images increased in misaligned images in the phantom study. In the human study, SPECT images were affected by the scan conditions of the X-ray CT. Attenuation correction of myocardial SPECT with an X-ray CT image is a simple and potentially beneficial method for clinical use, but accurate registration of the X-ray CT to SPECT image is essential for satisfactory attenuation correction. (author)

  9. Evaporation level of the condensate droplets on a shock wave in the IMP PAN nozzle depending on the inlet conditions

    Science.gov (United States)

    Kornet, S.; Badur, J.

    2016-10-01

    In the present paper we have focused on the phenomena of condensate reevaporation in the shock wave zone. Having observed the finishing of a foggy flow within the shock wave, according to Puzyrewski's observations, we would like to analyse the critical inlet conditions which cause total evaporation of condensate droplets on the shock in the IMP PAN nozzle. In the paper some original mechanistic model of droplet evaporation is involved, numerically implemented and compared with the IMP PAN experiment. The single continuum model of wet steam with a special microstructure growing up during phase transitions was validated on IMP PAN experiment performed for inlet conditions close to the saturation line. The present work includes simulations results of total and partial evaporation liquid phase on the shock wave for different boundary conditions.

  10. IMP-1 encoded by a novel Tn402-like class 1 integron in clinical Achromobacter xylosoxidans, China.

    Science.gov (United States)

    Chen, Zhenhong; Fang, Haihong; Wang, Li; Sun, Fengjun; Wang, Yong; Yin, Zhe; Yang, Huiying; Yang, Wenhui; Wang, Jie; Xia, Peiyuan; Zhou, Dongsheng; Liu, Changting

    2014-11-27

    Achromobacter xylosoxidans strain A22732 is isolated from a pneumonia patient in China and produces carbapenemases OXA-114e and IMP-1, which are encoded by chromosome and plasmid, respectively, and confer resistance to multiple ß-lactam antibiotics including carbapenems. The blaIMP-1 gene together with aacA7 and orfE is captured by a novel Tn402-like class 1 integron in a conjugative IncP-1ß plasmid. In addition to the intrinsic integron promoter PcW, there is still a blaIMP-1 gene cassette-specific promoter. This is the first report of carbapenemase-encoding IncP-1ß plasmid in clinical bacterial isolate.

  11. Lessons Learned from the Design, Certification, and Operation of the Space Shuttle Integrated Main Propulsion System (IMPS)

    Science.gov (United States)

    Martinez, Hugo E.; Albright, John D.; D'Amico, Stephen J.; Brewer, John M.; Melcher, John C., IV

    2011-01-01

    The Space Shuttle Integrated Main Propulsion System (IMPS) consists of the External Tank (ET), Orbiter Main Propulsion System (MPS), and Space Shuttle Main Engines (SSMEs). The IMPS is tasked with the storage, conditioning, distribution, and combustion of cryogenic liquid hydrogen (LH2) and liquid oxygen (LO2) propellants to provide first and second stage thrust for achieving orbital velocity. The design, certification, and operation of the associated IMPS hardware have produced many lessons learned over the course of the Space Shuttle Program (SSP). A subset of these items will be discussed in this paper for consideration when designing, building, and operating future spacecraft propulsion systems. This paper will focus on lessons learned related to Orbiter MPS and is the first of a planned series to address the subject matter.

  12. Impôts en France : réunion publique le mercredi 19 mars 2014

    CERN Document Server

    CERN. Geneva

    2014-01-01

    Impôts en France : réunion publique le mercredi 19 mars 2014 Une réunion publique aura lieu le mercredi 19 mars de 13h à 15h dans l’amphithéâtre principal (500/1-001). Au cours de cette réunion, MM. Jean-Louis Brandolin et Gérard Polizzi, respectivement responsable et responsable-adjoint du Service des impôts des particuliers et des entreprises de Bellegarde, Centre des finances publiques, apporteront leur expertise au sujet de l’imposition des membres du personnel du CERN domiciliés en France. Seront à l’ordre du jour les questions de principe que se posent apparemment de nombreux membres du personnel : par exemple, la définition du domicile fiscal, le contenu de la déclaration de revenus (mention des revenus CERN et déclaration des autres revenus de sources française ou étrangère), la déclaration des comptes bancaires à l’étranger, l’assujettissement (dans certaines conditions) à la CSG-CRDS. Cette réunion publique portera UNIQUEMENT sur des questions de principe ...

  13. {sup 67}Ga-SPECT/CT with a hybrid system in the clinical management of lymphoma

    Energy Technology Data Exchange (ETDEWEB)

    Palumbo, Barbara; Sivolella, Silvio; Palumbo, Renato [University of Perugia, Nuclear Medicine Section, Department of Radiological Sciences, Perugia (Italy); Palumbo, Isabella; Liberati, Anna Marina [University of Perugia, Internal Medicine and Oncology, Department of Clinical and Experimental Medicine, Perugia (Italy)

    2005-09-01

    The purpose of this study was to investigate the added value of co-registered fusion imaging using a hybrid system in patients with lymphoma. Twenty-four lymphoma patients underwent {sup 67}Ga-SPECT/CT using a hybrid tomograph consisting of a dual-head, variable-angle gamma camera and a low-dose X-ray tube. Results were compared with those of SPECT alone. Forty-five lesions were identified by SPECT alone, while 49 were detected by SPECT/CT. Forty out of the 45 lesions observed on SPECT were confirmed as lymphoma, but five were due to other causes (thoracic aorta blood pool activity, sialoadenitis in the submandibular gland, bowel activity, rib fracture and bone marrow activation due to radiotherapy). SPECT/CT identified nine more neoplastic lesions compared with SPECT alone: four areas of radiopharmaceutical accumulation were observed in para-aortic lymph nodes, three in the spleen, one in the liver and one in para-iliac lymph nodes. In five cases, SPECT/CT provided additional anatomical information over SPECT alone. In four patients, four large areas of {sup 67}Ga uptake (one mediastinal, two supraclavicular and one para-aortic) were better characterised; in one subject uptake was localised in the seventh thoracic vertebra only by SPECT/CT. Hybrid imaging provided additional data in 13 patients (54.2%), thus inducing oncologists to reconsider the therapeutic approach in eight subjects (33.2%): unnecessary treatment was avoided in four (16.6%) while therapy was altered in another four (16.6%). SPECT/CT hybrid system is able to provide information not obtained by SPECT alone. It allows the anatomical localisation of lymphoma and physiological radiopharmaceutical uptake, facilitates the diagnosis of tumours located in the abdomen (subdiaphragmatic lesions) and provides information that may cause a change in therapeutic strategy. (orig.)

  14. Brain SPECT in children; Explorations scintigraphiques en neurologie et psychiatrie de l`enfant

    Energy Technology Data Exchange (ETDEWEB)

    Guyot, M. [Hopital Pellegrin, 33 - Bordeaux (France); Baulieu, J.L. [Hopital Bretonneau, 37 - Tours (France)

    1996-12-31

    Brain SPECT in child involves specific trends regarding the patient cooperation, irradiation, resolution and especially interpretation because of the rapid scintigraphic modifications related to the brain maturation. In a general nuclear medicine department, child brain SPECT represents about 2 % of the activity. The choice indications are the perfusion children: thallium and MIBI in brain tumours, pharmacological and neuropsychological interventions. In the future, brain dedicated detectors and new radiopharmaceuticals will promote the development of brain SPECT in children. (author). 18 refs.

  15. Let-7 modulates acquired resistance of ovarian cancer to Taxanes via IMP-1-mediated stabilization of MDR1

    Science.gov (United States)

    Boyerinas, Benjamin; Park, Sun-Mi; Murmann, Andrea E.; Gwin, Katja; Montag, Anton G.; Zillardt, Marion R.; Hua, You-Jia; Lengyel, Ernst; Peter, Marcus E.

    2011-01-01

    Summary Ovarian cancer patients frequently develop resistance to chemotherapy regiments utilizing Taxol and carboplatin. One of the resistance factors that protects cancer cells from Taxol-based therapy is multi-drug resistance 1 (MDR1). micro(mi)RNAs are small noncoding RNAs that negatively regulate protein expression. Members of the let-7 family of miRNAs are downregulated in many human cancers, and low let-7 expression has been correlated with resistance to microtubule targeting drugs (Taxanes), although little is known that would explain this activity. We now provide evidence that, while let-7 is not a universal sensitizer of cancer cells to Taxanes, it affects acquired resistance of cells to this class of drugs by targeting IMP-1, resulting in de-stabilization of the mRNA of MDR1. Introducing let-7g into ADR-RES cells expressing both IMP-1 and MDR1 reduced expression of both proteins rendering the cells more sensitive to treatment with either Taxol or vinblastine without affecting the sensitivity of the cells to carboplatin, a non-MDR1 substrate. This effect could be reversed by reintroducing IMP-1 into let-7g high/MDR1 low cells causing MDR1 to again become stabilized. Consistently, many relapsed ovarian cancer patients tested before and after chemotherapy were found to downregulate let-7 and to co-upregulate IMP-1 and MDR1, and the increase in the expression levels of both proteins after chemotherapy negatively correlated with disease-free time before recurrence. Our data point at IMP-1 and MDR1 as indicators for response to therapy, and at IMP-1 as a novel therapeutic target for overcoming multidrug resistance of ovarian cancer. PMID:21618519

  16. Let-7 modulates acquired resistance of ovarian cancer to Taxanes via IMP-1-mediated stabilization of multidrug resistance 1.

    Science.gov (United States)

    Boyerinas, Benjamin; Park, Sun-Mi; Murmann, Andrea E; Gwin, Katja; Montag, Anton G; Zillhardt, Marion; Hua, You-Jia; Lengyel, Ernst; Peter, Marcus E

    2012-04-15

    Ovarian cancer patients frequently develop resistance to chemotherapy regiments using Taxol and carboplatin. One of the resistance factors that protects cancer cells from Taxol-based therapy is multidrug resistance 1 (MDR1). micro(mi)RNAs are small noncoding RNAs that negatively regulate protein expression. Members of the let-7 family of miRNAs are downregulated in many human cancers, and low let-7 expression has been correlated with resistance to microtubule targeting drugs (Taxanes), although little is known that would explain this activity. We now provide evidence that, although let-7 is not a universal sensitizer of cancer cells to Taxanes, it affects acquired resistance of cells to this class of drugs by targeting IMP-1, resulting in destabilization of the mRNA of MDR1. Introducing let-7g into ADR-RES cells expressing both IMP-1 and MDR1 reduced expression of both proteins rendering the cells more sensitive to treatment with either Taxol or vinblastine without affecting the sensitivity of the cells to carboplatin, a non-MDR1 substrate. This effect could be reversed by reintroducing IMP-1 into let-7g high/MDR1 low cells causing MDR1 to again become stabilized. Consistently, many relapsed ovarian cancer patients tested before and after chemotherapy were found to downregulate let-7 and to co-upregulate IMP-1 and MDR1, and the increase in the expression levels of both proteins after chemotherapy negatively correlated with disease-free time before recurrence. Our data point at IMP-1 and MDR1 as indicators for response to therapy, and at IMP-1 as a novel therapeutic target for overcoming multidrug resistance of ovarian cancer. Copyright © 2011 UICC.

  17. EANM procedural guidelines for radionuclide myocardial perfusion imaging with SPECT and SPECT/CT: 2015 revision

    Energy Technology Data Exchange (ETDEWEB)

    Verberne, Hein J.; Eck-Smit, Berthe L.F. van; Wit, Tim C. de [University of Amsterdam, Department of Nuclear Medicine, F2-238, Academic Medical Center, Amsterdam (Netherlands); Acampa, Wanda [National Council of Research, Institute of Biostructures and Bioimaging, Naples (Italy); Anagnostopoulos, Constantinos [Academy of Athens, Center for Experimental Surgery, Clinical and Translational Research, Biomedical Research Foundation, Athens (Greece); Ballinger, Jim [Guy' s Hospital - Guy' s and St Thomas' Trust Foundation, Department of Nuclear Medicine, London (United Kingdom); Bengel, Frank [Hannover Medical School, Department of Nuclear Medicine, Hannover (Germany); Bondt, Pieter De [OLV Hospital, Department of Nuclear Medicine, Aalst (Belgium); Buechel, Ronny R.; Kaufmann, Philip A. [University Hospital Zurich, Cardiac Imaging, Zurich (Switzerland); Cuocolo, Alberto [University Federico II, Department of Advanced Biomedical Sciences, Naples (Italy); Flotats, Albert [Universitat Autonoma de Barcelona, Nuclear Medicine Department, Hospital de la Santa Creu i Sant Pau, Barcelona (Spain); Hacker, Marcus [Medical University of Vienna, Division of Nuclear Medicine, Department of Biomedical Imaging and Image-Guided Therapy, Vienna (Austria); Hindorf, Cecilia [Skaane University Hospital, Department of Radiation Physics, Lund (Sweden); Lindner, Oliver [University Hospital of the Ruhr-University Bochum, Heart and Diabetes Center North Rhine-Westphalia, Institute for Radiology, Nuclear Medicine and Molecular Imaging, Bad Oeynhausen (Germany); Ljungberg, Michael [Lund University, Department of Medical Radiation Physics, Lund (Sweden); Lonsdale, Markus [Bispebjerg Hospital, Department of Clinical Physiology and Nuclear Medicine, Copenhagen (Denmark); Manrique, Alain [Caen University Hospital, Department of Nuclear Medicine, Service Commun Investigations chez l' Homme, GIP Cyceron, Caen (France); Minarik, David [Skaane University Hospital, Radiation Physics, Malmoe (Sweden); Scholte, Arthur J.H.A. [Leiden University Medical Center, Department of Cardiology, Leiden (Netherlands); Slart, Riemer H.J.A. [University of Groningen, University Medical Center Groningen, Department of Nuclear Medicine and Molecular Imaging, Groningen (Netherlands); Traegaardh, Elin [Skaane University Hospital and Lund University, Clinical Physiology and Nuclear Medicine, Malmoe (Sweden); Hesse, Birger [University Hospital of Copenhagen, Department of Clinical Physiology and Nuclear Medicine and PET, Rigshospitalet, Copenhagen (Denmark)

    2015-11-15

    Since the publication of the European Association of Nuclear Medicine (EANM) procedural guidelines for radionuclide myocardial perfusion imaging (MPI) in 2005, many small and some larger steps of progress have been made, improving MPI procedures. In this paper, the major changes from the updated 2015 procedural guidelines are highlighted, focusing on the important changes related to new instrumentation with improved image information and the possibility to reduce radiation exposure, which is further discussed in relation to the recent developments of new International Commission on Radiological Protection (ICRP) models. Introduction of the selective coronary vasodilator regadenoson and the use of coronary CT-contrast agents for hybrid imaging with SPECT/CT angiography are other important areas for nuclear cardiology that were not included in the previous guidelines. A large number of minor changes have been described in more detail in the fully revised version available at the EANM home page: http://eanm.org/ publications/guidelines/2015{sub 0}7{sub E}ANM{sub F}INAL myocardial{sub p}erfusion{sub g}uideline.pdf. (orig.)

  18. Evaluation of the use of freehand SPECT for sentinel node biopsy in early stage oral carcinoma.

    Science.gov (United States)

    Heuveling, Derrek A; van Weert, Stijn; Karagozoglu, K Hakki; de Bree, Remco

    2015-03-01

    Inadequate intraoperative visualization of the sentinel node can hamper its harvest. Freehand SPECT is a 3D tomographic imaging modality based on the concepts of SPECT, which can be used for intraoperative visualization and navigation towards the sentinel node in order to improve its localization and removal during surgery. The use of freehand SPECT was evaluated during 66 sentinel node biopsy procedures in early stage oral cancer patients. Intraoperative detection of sentinel nodes was compared with preoperative identified sentinel nodes on lymphoscinitigraphic examination. Additional value of freehand SPECT was subjectively scored by the surgeon directly following the biopsy procedure. Freehand SPECT was able to detect 94% of sentinel nodes intraoperatively. Most sentinel nodes not detected (7 out of 9) were located in level I of the neck. Freehand SPECT appeared to be of additional value for facilitating the intraoperative detection of the sentinel node in 24% of procedures. The use of the freehand SPECT system is feasible in the intraoperative detection of sentinel nodes in early stage oral cancer. Freehand SPECT provides helpful information facilitating the SN biopsy procedure in a quarter of cases. However, freehand SPECT cannot detect all SNs which are located in the vicinity of the injection site. Copyright © 2014 Elsevier Ltd. All rights reserved.

  19. DMSA SPECT imaging using oblique reconstruction in a paediatric population - benefits and technical considerations

    Energy Technology Data Exchange (ETDEWEB)

    Parsons, G.; Ford, M.; Crisp, J.; Bernard, E.; Howman-Giles, R. [The New Childrens Hospital, Westmead, NSW, (Australia). Department of Nuclear Medicine

    1997-09-01

    Full text: DMSA renal scans are frequently requested for the diagnosis and follow-up of acute pyelonephritis and cortical scarring. This study was designed to:- 1. evaluate oblique reconstruction of DMSA SPECT over standard plane reconstruction and planar imaging; and 2. report on the technical aspects important in obtaining high quality DMSA SPECT, particularly in neonates. Over seven months, 210/231 (91 %) of DMSA scans were performed with SPECT on children from age nine days to 16 years, the median age being 2.5 years. 65 patients (31 %) were under one year and 39 (18%) were under six months. Planar and SPECT imaging with standard plane reconstruction and oblique reorientation was performed on the Siemens triple-headed gamma camera. High quality SPECT images were obtained on the smallest babies using a paediatric palette, and were of comparable quality to those of older children. At the time of reporting, the nuclear medicine physician assessed the diagnostic value of the three types of date presented: (1) planar images; (2) standard plane SPECT reconstruction; and (3) oblique SPECT reconstruction. Cortical defects were identified separately for upper, middle and lower poles. Three physicians concluded that high quality SPECT is superior to planar images when assessing the renal cortex. In addition, oblique reorientation is superior to standard reconstruction, particularly at the upper and lower poles. SPECT is now performed routinely on patients of all ages, and the oblique sagittal and coronal reorientation is now used in place of the standard reconstruction.

  20. Processamento auditivo e SPECT em crianças com dislexia Dichotic listening and spect in dyslexic children

    Directory of Open Access Journals (Sweden)

    Luciane Sauer

    2006-03-01

    Full Text Available Aplicamos em um grupo de 36 crianças os testes dicótico de dígitos, dicótico de dissílabos alternados e dicótico não-verbal, os quais fazem parte do conjunto de avaliação do processamento auditivo. As crianças foram divididas em dois grupos que foram correlacionados: grupo experimental (GE formado por 18 crianças com diagnóstico de dislexia e 18 crianças normais compondo o grupo comparação (GC, sem queixa de aprendizagem e pareadas em relação a sexo, lateralidade e nível sócio-econômico ao GE. Realizamos o exame de imagem (SPECT no GE. Encontramos em nosso estudo diferença estatisticamente significante entre os grupos (GE e GC em todos os testes avaliados. Em relação ao SPECT, apesar de não encontrarmos diferença estatisticamente significante entre os resultados, pudemos observar que 50% dos sujeitos avaliados apresentaram alteração, sendo a maior parte em áreas do lobo temporal esquerdo. Concluímos que crianças com dislexia apresentam alterações do processamento neurológico central que podem ser detectadas tanto em testes específicos de processamento auditivo, quanto em exames funcionais de imagem como SPECT.Dichotic listening (DL was evaluated in 36 children with: verbal dichotic listening test, alternating dissilable dichotic test and non-verbal dichotic listening test. Children were separated into two groups: experimental group with 18 dyslexic children and control group with 18 normal children. Both groups were comparable in gender, laterality and social-economic level. All dyslexic children underwent neuroimaging exam (SPECT. Our data showed that there was a statistical difference between both groups in all DL tests. Abnormal SPECT findings were seen in 50% of the dyslexic children, hypoperfusion of the left temporal lobe being the most frequent abnormality. We conclude that dyslexic children present an impairment of central neurologic processing that may be detected by DL tests, and by functional imaging

  1. Recent advances in cardiac SPECT instrumentation and system design.

    Science.gov (United States)

    Smith, Mark F

    2013-08-01

    Recent advances in clinical cardiac SPECT instrumentation are reviewed from a systems perspective. New hardware technologies include pixelated scintillator and semiconductor detector elements; photodetectors such as position-sensitive photomultiplier tubes (PSPMT), avalanche photodiodes (APD) and silicon photomultipliers (SiPM); and novel cardiac collimation methods. There are new approaches for positioning detectors and controlling their motion during cardiac imaging. Software technology advances include iterative image reconstruction with modeling of Poisson statistics and depth-dependent collimator response. These new technologies enable faster acquisitions, the lowering of administered activity and radiation dose, and improved image resolution. Higher sensitivity collimators are a significant factor enabling faster acquisitions. Several clinical systems incorporating new technologies are discussed and different system designs can achieve similar performance. With detector elements such as APDs, SiPMs and semiconductors that are insensitive to magnetic fields, the potential for cardiac SPECT imagers that are MRI compatible opens up new frontiers in clinical cardiac research and patient care.

  2. Status report of the neutron lifetime experiment tau-SPECT

    Energy Technology Data Exchange (ETDEWEB)

    Karch, Jan Peter; Beck, Marcus; Dragisic, Simo; Haak, Jan; Heil, Werner; Kories, Fabian; Kunz, Simon; Stepanow, Dietmar [Institut fuer Physik, University of Mainz (Germany); Geppert, Christopher; Karpuk, Sergei [Institut fuer Kernchemie, University of Mainz (Germany); Sobolev, Yury [Institut fuer Physik, University of Mainz (Germany); Institut fuer Kernchemie, University of Mainz (Germany)

    2015-07-01

    The decay of the free neutron into a proton, electron and antineutrino is the prototype of the semi-leptonic weak decay and plays a key role in particle physics and astrophysics. Nowadays, the accuracy achieved is limited by systematic errors, mainly caused by anomalous losses during storage of neutrons (ultracold neutrons) in material vessels. The magnetic storage of neutrons aims to avoid these systematic limitations and is expected to reach an accuracy of 0.1-0.3 s in the lifetime of the neutron. In this talk, the magnetic spectrometer tau-SPECT is presented, which uses a combination of magnetic multipole fields for radial storage and the field configuration of the superconducting aSPECT magnet for longitudinal storage of ultracold neutrons. This storage experiment benefits greatly from the new ultracold neutron source at the pulsable TRIGA reactor Mainz. The talk gives an overview of the experimental status: Proton detection system and adiabatic fast passages device.

  3. Results and present status of the spectrometer aSPECT

    Energy Technology Data Exchange (ETDEWEB)

    Ayala Guardia, Fidel; Borg, Michael; Glueck, Ferenc; Heil, Werner; Konrad, Gertrud; Munoz Horta, Raquel; Sobolev, Yury [Institut fuer Physik, Universitaet Mainz (Germany); Angerer, Heinz; Konorov, Igor; Petzoldt, Gerd; Simson, Martin; Wirth, Hans-Friedrich; Zimmer, Oliver [Physik Department, Technische Universitaet Muenchen (Germany); Baessler, Stefan [Department of Physics, University of Virginia, Charlottesville (United States); Eberhardt, Klaus [Institut fuer Kernchemie, Universitaet Mainz (Germany)

    2008-07-01

    The aim of the aSPECT spectrometer is a precise measurement of the proton recoil spectrum in free neutron decay. For kinematic reasons, the shape of the proton spectrum depends on the angular correlation coefficient between the momenta of the electron antineutrino and the electron, a. An accurate measurement of the angular correlation coefficient a is of great interest in order to test the unitarity of the Cabibbo Kobayashi Maskawa Matrix (CKM-Matrix). A first test beam time was performed during 2005/06 at the beam line MEPHISTO at the neutron research reactor FRM-II in Garching. Results of the data analysis as well as the present status of the aSPECT spectrometer are presented in this talk.

  4. aSPECT - Measuring the proton spectrum in neutron decay

    Energy Technology Data Exchange (ETDEWEB)

    Simson, Martin; Soldner, Torsten; Zimmer, Oliver [Institut Laue-Langevin, Grenoble (France); Physik-Department E18, TU Muenchen (Germany); Ayala Guardia, Fidel; Borg, Michael; Heil, Werner; Konrad, Gertrud; Munoz Horta, Raquel; Ostrick, Beatrix [Institut fuer Physik, Universitaet Mainz (Germany); Baessler, Stefan [University of Virginia, Charlottesville, VA (United States); Glueck, Ferenc [IEKP, Universitaet Karlsruhe (Germany); Konorov, Igor [Physik-Department E18, TU Muenchen (Germany); Wirth, Hans-Friedrich [Fakultaet fuer Physik, LMU Muenchen (Germany)

    2010-07-01

    With the aSPECT spectrometer we measure the proton recoil spectrum in the decay of the free neutron. Its shape depends on the angular correlation between the momenta of the antineutrino and electron for kinematic reasons. A precision measurement of this correlation coefficient a allows to test the unitarity of the CKM matrix and provides limits on the existence of scalar and tensor currents. aSPECT is a retardation spectrometer, this means protons from neutron decay are guided by a strong magnetic field and the proton recoil spectrum is measured by counting all protons that overcome a electrostatic barrier. By varying the height of the barrier the shape of the proton spectrum can be reconstructed. After the barrier the protons are accelerated to {proportional_to}15 keV and detected by a silicon drift detector. This talk covers details of the spectrometer and detector, as well as techniques used in the data analysis.

  5. HM-PAO-SPECT in complicated migraine. Case report

    Energy Technology Data Exchange (ETDEWEB)

    Gruenwald, F.; Ruhlmann, J.; Biersack, H.J.; Durwen, H.; Penin, H.

    1988-10-01

    Three HM-PAO-SPECT investigations have been performed in a female patient with a complicated migraine. 2.5 hours after an attack with right-sided numbness and atonic hemiparesis, at the left side a reduced blood flow was seen parietotemporally and in the subcortical structures. The cerebellum showed crossed diaschisis. During a symptom-free interval the SPECT showed a slightly increased parietotemporal blood blow and a reduced right temporooccipital blood flow. During a seizure with left-sided facial spasm, unsystematic clonic movements in all extremities and a following left-sided hemiparesis, the uptake in the visual cortex was increased by 60%. In the right frontotemporal and left temporal region a slightly increased accumulation was found.

  6. Infective endocarditis detection through SPECT/CT images digital processing

    Science.gov (United States)

    Moreno, Albino; Valdés, Raquel; Jiménez, Luis; Vallejo, Enrique; Hernández, Salvador; Soto, Gabriel

    2014-03-01

    Infective endocarditis (IE) is a difficult-to-diagnose pathology, since its manifestation in patients is highly variable. In this work, it was proposed a semiautomatic algorithm based on SPECT images digital processing for the detection of IE using a CT images volume as a spatial reference. The heart/lung rate was calculated using the SPECT images information. There were no statistically significant differences between the heart/lung rates values of a group of patients diagnosed with IE (2.62+/-0.47) and a group of healthy or control subjects (2.84+/-0.68). However, it is necessary to increase the study sample of both the individuals diagnosed with IE and the control group subjects, as well as to improve the images quality.

  7. IMP3 RNP safe houses prevent miRNA-directed HMGA2 mRNA decay in cancer and development

    DEFF Research Database (Denmark)

    Jønson, Lars; Christiansen, Jan; Hansen, Thomas van Overeem

    2014-01-01

    embryos show dose-dependent Hmga2 mRNA downregulation. Finally, IMP3 ribonucleoproteins (RNPs) contain other let-7 target mRNAs, including LIN28B, and a global gene set enrichment analysis demonstrates that miRNA-regulated transcripts in general are upregulated following IMP3 induction. We conclude...

  8. Coproduction of KPC-2 and IMP-10 in Carbapenem-Resistant Serratia marcescens Isolates from an Outbreak in a Brazilian Teaching Hospital.

    Science.gov (United States)

    Silva, Kesia Esther; Cayô, Rodrigo; Carvalhaes, Cecilia Godoy; Patussi Correia Sacchi, Flávia; Rodrigues-Costa, Fernanda; Ramos da Silva, Ana Carolina; Croda, Julio; Gales, Ana Cristina; Simionatto, Simone

    2015-07-01

    We describe an outbreak caused by KPC-2- and IMP-10-producing Serratia marcescens isolates in a Brazilian teaching hospital. Tigecycline was the only active antimicrobial agent tested. The blaIMP-10 gene was located in a new class 1 integron, named In990, carried by a nonconjugative plasmid, in contrast to blaKPC-2.

  9. Coproduction of KPC-2 and IMP-10 in Carbapenem-Resistant Serratia marcescens Isolates from an Outbreak in a Brazilian Teaching Hospital

    Science.gov (United States)

    Silva, Kesia Esther; Cayô, Rodrigo; Carvalhaes, Cecilia Godoy; Patussi Correia Sacchi, Flávia; Rodrigues-Costa, Fernanda; Ramos da Silva, Ana Carolina; Croda, Julio; Gales, Ana Cristina

    2015-01-01

    We describe an outbreak caused by KPC-2- and IMP-10-producing Serratia marcescens isolates in a Brazilian teaching hospital. Tigecycline was the only active antimicrobial agent tested. The blaIMP-10 gene was located in a new class 1 integron, named In990, carried by a nonconjugative plasmid, in contrast to blaKPC-2. PMID:25878341

  10. Transient Crossed Aphasia: A Case Study with SPECT

    Directory of Open Access Journals (Sweden)

    José M. Ferro

    1991-01-01

    Full Text Available A 45-year-old right-handed man developed “crossed” anomie aphasia, neglect and inaccurate reaching of his left arm in both hemispaces due to a right parietal haemorrhage. Aphasia cleared in a few days. SPECT failed to demonstrate crossed left hemispheric diaschisis. This report emphasizes the importance of evaluating patients in the acute stage and of coupling “static” and “functional” imaging methods when studying brain-behavior relationships.

  11. Evaluation of pelvic ring injuries using SPECT/CT

    Energy Technology Data Exchange (ETDEWEB)

    Scheyerer, Max J. [University Hospital Zurich, Division of Trauma Surgery, Department of Surgery, Zurich (Switzerland); University Medical Center, Centre for Orthopaedic and Trauma Surgery, Cologne (Germany); Huellner, Martin; Pietsch, Carsten [University Hospital Zurich, Division of Nuclear Medicine, Department of Medical Radiology, Zurich (Switzerland); Werner, Clement M.L. [University Hospital Zurich, Division of Trauma Surgery, Department of Surgery, Zurich (Switzerland); Veit-Haibach, Patrick [University Hospital Zurich, Division of Nuclear Medicine, Department of Medical Radiology, Zurich (Switzerland); University Hospital Zurich, Diagnostic and Interventional Radiology, Department of Medical Radiology, Zurich (Switzerland)

    2014-08-12

    The incidence of pelvic fractures is relatively low compared with other fracture locations. The low incidence is in great contrast to the high morbidity and mortality. Particularly in the elderly, with apparently isolated fractures of the pubic rami, these observations are believed to be due to additional occult lesions of the posterior pelvic ring. In these cases diagnosis cannot be established by conventional imaging alone and SPECT/CT is considered as a diagnostic adjunct. The aim of this study was to assess concomitant bony or soft tissue lesions within the pelvic ring in a population of patients with fractures of the anterior elements. In all patients with no obvious lesions of the posterior pelvic ring on X-rays and CT or with suspicious but inconclusive findings on CT an additional SPECT/CT was carried out in a non-acute setting within 3 days of the trauma. In all cases additional lesions within the pelvic ring were found. Most lesions were vertical sacral fractures, followed by transverse fractures, one non-dislocated fracture of the acetabulum on the side of the pubic rami fracture, and one post-traumatic dilatation of the sacroiliac joint with increased tracer uptake. According to our results after SPECT/CT all patients with pubic rami fracture suffered additional lesions, none detectable previously by X-ray or CT, within the pelvic ring. In this context SPECT/CT has proved to be very helpful in the clinical routine to visualize occult fractures and instability within the sacroiliac joint. (orig.)

  12. In vivo SPECT reporter gene imaging of regulatory T cells.

    Directory of Open Access Journals (Sweden)

    Ehsan Sharif-Paghaleh

    Full Text Available Regulatory T cells (Tregs were identified several years ago and are key in controlling autoimmune diseases and limiting immune responses to foreign antigens, including alloantigens. In vivo imaging techniques including intravital microscopy as well as whole body imaging using bioluminescence probes have contributed to the understanding of in vivo Treg function, their mechanisms of action and target cells. Imaging of the human sodium/iodide symporter via Single Photon Emission Computed Tomography (SPECT has been used to image various cell types in vivo. It has several advantages over the aforementioned imaging techniques including high sensitivity, it allows non-invasive whole body studies of viable cell migration and localisation of cells over time and lastly it may offer the possibility to be translated to the clinic. This study addresses whether SPECT/CT imaging can be used to visualise the migratory pattern of Tregs in vivo. Treg lines derived from CD4(+CD25(+FoxP3(+ cells were retrovirally transduced with a construct encoding for the human Sodium Iodide Symporter (NIS and the fluorescent protein mCherry and stimulated with autologous DCs. NIS expressing self-specific Tregs were specifically radiolabelled in vitro with Technetium-99m pertechnetate ((99mTcO(4(- and exposure of these cells to radioactivity did not affect cell viability, phenotype or function. In addition adoptively transferred Treg-NIS cells were imaged in vivo in C57BL/6 (BL/6 mice by SPECT/CT using (99mTcO(4(-. After 24 hours NIS expressing Tregs were observed in the spleen and their localisation was further confirmed by organ biodistribution studies and flow cytometry analysis. The data presented here suggests that SPECT/CT imaging can be utilised in preclinical imaging studies of adoptively transferred Tregs without affecting Treg function and viability thereby allowing longitudinal studies within disease models.

  13. The relationship of Imp2 and DR3 genes with susceptibility to type Ⅰ diabetes mellitus in south China Han population

    Institute of Scientific and Technical Information of China (English)

    He Lin Ding; Hua Cheng; Zu Zhi Fu; Qing Li Deng; Li Yan; Tang Yan

    2000-01-01

    AIM To study the relationship of Imp2 and DR3 genes with type Ⅰ diabetes mellitus.METHODS Imp2 genotypes and DR3 were identified in 68 patients with type Ⅰ diabetes mellitus ( Ⅰ-DM) and 71 healthy controls. Then,Ⅰ-DM patients and controls were respectively allocated into DR3-positive and DR3-negative groups. The frequencies of Imp2 and DR3 gene in random subjects, and Imp2 genotypes in DR3-matched subjects were compared between Ⅰ -DM patients and controls. At the same time, Ⅰ-DM patients were divided into 3 groups based on the onset age of diabetics: group A ≤ 14 years,group B 15- 30 years and group C≥31 years.RESULTS The frequency of DR3 in Ⅰ-DM patients was significantly higher than that in controls (47% vs 21%, P<0.005), and it was significantly higher in group A than that in group B+C (70% vs 36%, x2=7.07, P<0.01). There was a significant difference among groups with different onset age of diabetics (x2=8.19,rp=0.33, P<0.05). In random subjects, thefrequency of Imp2-R/R in Ⅰ-DM patients was lower (43% vs 61%, P<0.05) and Imp2-R/H higher (53% vs 28%, P<0.05) than that in controls, and there was no significant difference among groups with different onset age of diabetics. In DR3-positive subjects, the frequency of Imp2-R/R in Ⅰ-DM patients was lower (47% vs 87%, P<0.05) and Imp2-R/H higher (47% vs 13%, P < 0.05) than that in controls. In DR3-negative subjects, the frequency of Imp2-R/H in Ⅰ-DM patients was higher than that in controls (58% vs 32%, P<0.01), but the frequency of Imp2-R/R and Imp2 H/H was not significantly different between these two groups.CONCLUSION DR3 gene may be one of the susceptible genes of Ⅰ-DM, and significantly related to the onset age of diabetics, and the persons with DR3 may have an younger onset age of diabetes. The Imp2-R/R may be the protective genotype of Ⅰ-DM, and Imp2-R/H the susceptible genotype. These were not affected by DR3 gene. Imp-2 genotypes were not related with the onset age

  14. Physiological imaging with PET and SPECT in Dementia

    Energy Technology Data Exchange (ETDEWEB)

    Jagust, W.J. (California Univ., San Francisco, CA (United States). Dept. of Neurology Lawrence Berkeley Lab., CA (United States))

    1989-10-01

    Dementia is a medical problem of increasingly obvious importance. The most common cause of dementia, Alzheimer's disease (AD) accounts for at least 50% of all cases of dementia, with multi-infarct dementia the next most common cause of the syndrome. While the accuracy of diagnosis of AD may range from 80 to 90%, there is currently no laboratory test to confirm the diagnosis. Functional imaging techniques such as positron emission tomography (PET) and single photon emission computed tomography (SPECT) offer diagnostic advantages since brain function is unequivocally disturbed in all dementing illnesses. Both PET and SPECT have been utilized in the study of dementia. While both techniques rely on principles of emission tomography to produce three dimensional maps of injected radiotracers, the differences between positron and single photon emission have important consequences for the practical applications of the two procedures. This briefly reviews the technical differences between PET and SPECT, and discusses how both techniques have been used in our laboratory to elucidate the pathophysiology of dementia. 32 refs., 2 figs.

  15. SVD-Based Evaluation of Multiplexing in Multipinhole SPECT Systems

    Directory of Open Access Journals (Sweden)

    Aaron K. Jorgensen

    2008-01-01

    Full Text Available Multipinhole SPECT system design is largely a trial-and-error process. General principles can give system designers a general idea of how a system with certain characteristics will perform. However, the specific performance of any particular system is unknown before the system is tested. The development of an objective evaluation method that is not based on experimentation would facilitate the optimization of multipinhole systems. We derive a figure of merit for prediction of SPECT system performance based on the entire singular value spectrum of the system. This figure of merit contains significantly more information than the condition number of the system, and is therefore more revealing of system performance. This figure is then compared with simulated results of several SPECT systems and is shown to correlate well to the results of the simulations. The proposed figure of merit is useful for predicting system performance, but additional steps could be taken to improve its accuracy and applicability. The limits of the proposed method are discussed, and possible improvements to it are proposed.

  16. Advances in SPECT imaging with respect to radionuclide therapy.

    Science.gov (United States)

    D'Asseler, Y

    2009-06-01

    Radionuclide therapy is gradually becoming more important as a therapy option in various diseases. Nuclear medicine imaging plays an important role in this, before, during and after the therapy. Single photon emission computed tomography (SPECT) imaging can be used to predict therapy response, calculate doses delivered to the tumour and the surrounding organ, check radiopharmaceutical distribution and follow-up this distribution in time. On a technological level, radionuclide imaging in a therapy setting shows some particularities and issues to be resolved. Accurate quantification is important but is hampered by attenuation, scatter from different energy peaks and from bremsstrahlung photons, septal penetration, partial volume effects etc. Some of these issues are discussed in this paper. A technique specific for therapy imaging is bremsstrahlung imaging, which can be used if the therapeutical agent is a pure beta emitter. Quantitative bremsstrahlung imaging is particularly challenging due to the complicated nature of the energy spectrum of these photons. Some work towards quantitative bremsstrahlung imaging is discussed here. Finally, some recent technical advances relevant to this field are pointed out. On the software side, Monte Carlo simulations seem to have a great potential for accurate quantitative SPECT reconstruction and subsequent patient specific image based dose calculations. Concerning hardware, the availability of SPECT-CT technology may have a large impact in imaging in radionuclide therapy. Novel detector technologies such as solid-state detectors may also prove to have significant advantages in this field.

  17. Grafted bone used in acetabular reconstruction: assessment by SPECT scintigraphy

    Energy Technology Data Exchange (ETDEWEB)

    Fujikawa, Yosuke [Dept. of Orthopaedic Surgery, Oita Medical Univ. (Japan); Torisu, Takehiko [Dept. of Orthopaedic Surgery, Oita Medical Univ. (Japan); Takasita, Mitsuhiro [Dept. of Orthopaedic Surgery, Oita Medical Univ. (Japan); Masumi, Shogo [Dept. of Orthopaedic Surgery, Oita Medical Univ. (Japan)

    1995-08-01

    Single photon emission computed tomography (SPECT) was carried out at the 1st, 6th, and 12th month after the operation and the uptakes on serial SPECT scintigrams were normalized. The horizontal histogram of scintigraphic activity at the level of the central grafted bone in the coronal image was designated the profile curve. Seven patients who had undergone bipolar hip arthroplasty for osteoarthritis of the hip were randomly selected. Five of seven profile curves obtained at the 1st month demonstrated a single peak at the host-graft junction. The profile curve obtained at the 6th month showed two peaks at the host-graft junction and the priphery of the grafted bone in all patients. The profile curve of the 12th month showed slightly increased activity in the center of the grafted bone. This preliminary study demonstrates that comparison of the serial profile curves on SPECT is valuable in evaluating the progress of incorporation of the grafted bone in hip arthroplasty. (orig.)

  18. [Usefulness of attenuation correction with transmission source in myocardial SPECT].

    Science.gov (United States)

    Murakawa, Keizo; Katafuchi, Tetsuro; Nishimura, Yoshihiro; Enomoto, Naoyuki; Sago, Masayoshi; Oka, Hisashi

    2006-01-20

    Attenuation correction in SPECT has been used for uniformly absorptive objects like the head. On the other hand, it has seldom been applied to nonuniform absorptive objects like the heart and surrounding lungs because of the difficulty and inaccuracy of data processing. However, since attenuation correction using a transmission source recently became practical, we were able to apply this method to a nonuniform absorptive object. Therefore, we evaluated the usefulness of this attenuation correction system with a transmission source in myocardial SPECT. The dose linearity, defect/normal ratio using a myocardial phantom, and myocardial count distribution in clinical cases was examined with and without the attenuation correction system. We found that all data processed with attenuation correction were better than those without attenuation correction. For example, in myocardial count distribution, while there was a difference between men and women without attenuation correction, which was considered to be caused by differences in body shape, after processing with attenuation correction, myocardial count distribution was almost the same in all cases. In conclusion, these results suggested that attenuation correction with a transmission source was useful in myocardial SPECT.

  19. Novel SPECT Technologies and Approaches in Cardiac Imaging

    Directory of Open Access Journals (Sweden)

    Piotr Slomka

    2016-12-01

    Full Text Available Recent novel approaches in myocardial perfusion single photon emission CT (SPECT have been facilitated by new dedicated high-efficiency hardware with solid-state detectors and optimized collimators. New protocols include very low-dose (1 mSv stress-only, two-position imaging to mitigate attenuation artifacts, and simultaneous dual-isotope imaging. Attenuation correction can be performed by specialized low-dose systems or by previously obtained CT coronary calcium scans. Hybrid protocols using CT angiography have been proposed. Image quality improvements have been demonstrated by novel reconstructions and motion correction. Fast SPECT acquisition facilitates dynamic flow and early function measurements. Image processing algorithms have become automated with virtually unsupervised extraction of quantitative imaging variables. This automation facilitates integration with clinical variables derived by machine learning to predict patient outcome or diagnosis. In this review, we describe new imaging protocols made possible by the new hardware developments. We also discuss several novel software approaches for the quantification and interpretation of myocardial perfusion SPECT scans.

  20. SVD-based evaluation of multiplexing in multipinhole SPECT systems.

    Science.gov (United States)

    Jorgensen, Aaron K; Zeng, Gengsheng L

    2008-01-01

    Multipinhole SPECT system design is largely a trial-and-error process. General principles can give system designers a general idea of how a system with certain characteristics will perform. However, the specific performance of any particular system is unknown before the system is tested. The development of an objective evaluation method that is not based on experimentation would facilitate the optimization of multipinhole systems. We derive a figure of merit for prediction of SPECT system performance based on the entire singular value spectrum of the system. This figure of merit contains significantly more information than the condition number of the system, and is therefore more revealing of system performance. This figure is then compared with simulated results of several SPECT systems and is shown to correlate well to the results of the simulations. The proposed figure of merit is useful for predicting system performance, but additional steps could be taken to improve its accuracy and applicability. The limits of the proposed method are discussed, and possible improvements to it are proposed.

  1. Concordance between myocardial perfusion scan assessed by SPECT and fractional flow reserve findings for detection of significant ischemia

    Directory of Open Access Journals (Sweden)

    Morteza Safi

    2016-09-01

    Conclusion: FFR and MPI with SPECT techniques showed significant concordance for detection of myocardial ischemia, regardless of the type of diseased coronary arteries. In this context, SPECT has high sensitivity and NPV for detection of ischemia compared with FFR.

  2. A new method to evaluate ischemic heart disease. Combined use of rest thallium-201 myocardial SPECT and Tc-99m exercise tetrofosmin first pass and myocardial SPECT

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Yong-ih; Goto, Hideki; Kobayashi, Katsuhiro; Sawada, Yoshihiro; Miyake, Yoshitaka; Fujiwara, Go [Nishiyodo Hospital, Osaka (Japan); Okada, Tomoya; Nishimura, Tsunehiko

    1999-06-01

    We developed a new diagnostic method for simultaneously evaluating myocardial ischemia, myocardial viability and ventricular function in less than 90 minutes by combined use of rest thallium-201 (Tl) SPECT and exercise Tc-99m tetrofosmin (TF) first pass and SPECT. The subjects were 9 healthy controls, 19 angina pectoris patients, and 19 old myocardial infarction patients, in all of whom coronary angiography had been performed. Rest Tl myocardial SPECT was performed first, and was followed by exercise TF myocardial SPECT. We also performed first pass radionuclide angiography by TF during maximum exercise on a bicycle ergometer to assess the left ventricular ejection fraction (LVEF). The total examination time was less than 90 minutes. SPECT diagnosis was performed by semi-quantitative analysis. LVEF below 55% was regarded as abnormal. In the patients with angina pectoris, analysis according to the coronary artery showed that the diagnostic accuracy of SPECT was 85.0% for ischemia in the region of the left anterior descending branch (LAD), 87.5% for the left circumflex branch (LCX) and 77.8% for the right coronary artery (RCA). The accuracy of diagnosis for angina pectoris was 82.1%, as determined by SPECT alone, and rose to 89,3% when the LVEF levels were also taken into consideration. In the patients with old myocardial infarction, the diagnostic accuracy of SPECT was 84.2% for the LAD, 92.3% for the LCX and 85.0% for the RCA. Analysis by patients showed that the accuracy of diagnosis for myocardial infarction was 85.7%, as determined by SPECT alone. The diagnostic accuracy, however, rose to 89.3% when the LVEF levels also were taken into consideration. In conclusion, it was demonstrated that this combined diagnostic method was highly reliable for evaluating ischemic heart disease within a short time. (author)

  3. 3D SPECT/CT fusion using image data projection of bone SPECT onto 3D volume-rendered CT images: feasibility and clinical impact in the diagnosis of bone metastasis.

    Science.gov (United States)

    Ogata, Yuji; Nakahara, Tadaki; Ode, Kenichi; Matsusaka, Yohji; Katagiri, Mari; Iwabuchi, Yu; Itoh, Kazunari; Ichimura, Akira; Jinzaki, Masahiro

    2017-05-01

    We developed a method of image data projection of bone SPECT into 3D volume-rendered CT images for 3D SPECT/CT fusion. The aims of our study were to evaluate its feasibility and clinical usefulness. Whole-body bone scintigraphy (WB) and SPECT/CT scans were performed in 318 cancer patients using a dedicated SPECT/CT systems. Volume data of bone SPECT and CT were fused to obtain 2D SPECT/CT images. To generate our 3D SPECT/CT images, colored voxel data of bone SPECT were projected onto the corresponding location of the volume-rendered CT data after a semi-automatic bone extraction. Then, the resultant 3D images were blended with conventional volume-rendered CT images, allowing to grasp the three-dimensional relationship between bone metabolism and anatomy. WB and SPECT (WB + SPECT), 2D SPECT/CT fusion, and 3D SPECT/CT fusion were evaluated by two independent reviewers in the diagnosis of bone metastasis. The inter-observer variability and diagnostic accuracy in these three image sets were investigated using a four-point diagnostic scale. Increased bone metabolism was found in 744 metastatic sites and 1002 benign changes. On a per-lesion basis, inter-observer agreements in the diagnosis of bone metastasis were 0.72 for WB + SPECT, 0.90 for 2D SPECT/CT, and 0.89 for 3D SPECT/CT. Receiver operating characteristic analyses for the diagnostic accuracy of bone metastasis showed that WB + SPECT, 2D SPECT/CT, and 3D SPECT/CT had an area under the curve of 0.800, 0.983, and 0.983 for reader 1, 0.865, 0.992, and 0.993 for reader 2, respectively (WB + SPECT vs. 2D or 3D SPECT/CT, p < 0.001; 2D vs. 3D SPECT/CT, n.s.). The durations of interpretation of WB + SPECT, 2D SPECT/CT, and 3D SPECT/CT images were 241 ± 75, 225 ± 73, and 182 ± 71 s for reader 1 and 207 ± 72, 190 ± 73, and 179 ± 73 s for reader 2, respectively. As a result, it took shorter time to read 3D SPECT/CT images than 2D SPECT/CT (p < 0.0001) or WB + SPECT

  4. IMP3 expression in lesions of the biliary tract: a marker for high-grade dysplasia and an independent prognostic factor in bile duct carcinomas.

    Science.gov (United States)

    Riener, Marc-Oliver; Fritzsche, Florian R; Clavien, Pierre-Alain; Pestalozzi, Bernhard C; Probst-Hensch, Nicole; Jochum, Wolfram; Kristiansen, Glen

    2009-10-01

    The oncofetal protein IMP3 (insulin-like growth factor II mRNA binding protein 3) is expressed during embryogenesis and carcinogenesis. Various tumor types have been analyzed for IMP3 expression, which was exclusively found in tumor cells and correlated with increased tumor aggressiveness and reduced overall survival. To our knowledge, IMP3 expression has not been investigated in bile duct carcinomas. Using large tissue sections from resection specimens of the extrahepatic biliary tract, we analyzed IMP3 in normal bile ducts (n = 36), bile ducts with acute inflammation and reactive epithelial changes (n = 26), low-grade dysplasia (n = 9), and high-grade dysplasia (n = 11). Furthermore, IMP3 expression was assessed in bile duct carcinoma (n = 115) using clinically well-characterized tissue microarrays. The findings were correlated with clinical-pathologic parameters including survival. High-grade dysplasia was strongly positive for IMP3 in all cases studied compared with no or weak expression in normal, inflamed, and low-grade dysplastic bile ducts. Of the bile duct carcinomas 58.3% (67/115) were strongly positive for IMP3, which was associated with a higher proliferation rate (P = .004) and p53 positivity (P = .022). Patients with strong IMP3 expression had significantly reduced overall survival (P = .037) similarly to the subgroup of pT3 carcinomas (P = .007). In multivariate analysis, IMP3 expression was an independent prognostic factor for overall survival (P = .040, RR = 1.809). This comprehensive study shows that IMP3 is an independent prognostic biomarker in bile duct carcinoma. In addition, it may be a marker for high-grade dysplasia in the extrahepatic biliary tract.

  5. Improved diagnostic accuracy of lung perfusion imaging using Tc-99m MAA SPECT

    Energy Technology Data Exchange (ETDEWEB)

    O' Donnell, J.K.; Golish, J.A.; Go, R.T.; Risius, B.; Graor, R.A.; MacIntyre, W.J.; Feiglin, D.H.

    1984-01-01

    The addition of emission tomography (SPECT) to pulmonary perfusion imaging should improve diagnostic accuracy by detecting perfusion defects otherwise masked by superimposition of normal lung activity and by reducing problems with interpretation of defects that result from overlying soft tissue or pleural effusions. In order to examine the contribution of SPECT in the scintigraphic evaluation for pulmonary embolus (PE), the authors have obtained both planar and SPECT studies in 94 cases of suspected PE. All studies employed 3-4 mCi of Tc-99m MAA and standard six-view planar image acquisition. SPECT raw data of 64 images were then acquired over a 360 degree transaxial rotation with subsequent computer reconstruction. Xe-133 ventilation studies were performed when clinically indicated and tolerated by the patient. For 19 studies angiographic (AN) correlation was obtained within 24 hours. In 16/19 planar and SPECT both gave a high probability of PE but SPECT gave better segmental localization and showed better agreement with the number of defects seen at AN. In 3 indeterminate planar scans, 2 were low probability with SPECT and had negative AN. The third, a patient with Wegener's vasculitis, remained indeterminate with SPECT and had negative AN. Five patients with PE had repeat planar/SPECT/AN studies to evaluate response to treatment. SPECT correlated better with AN findings in each case. The authors conclude that SPECT perfusion imaging provides better anatomic accuracy for defects representing PE and is the non-invasive technique of choice for documenting response to therapy.

  6. Quantifying the Increase in Radiation Exposure Associated with SPECT/CT Compared to SPECT Alone for Routine Nuclear Medicine Examinations

    Directory of Open Access Journals (Sweden)

    Ann M. Larkin

    2011-01-01

    Full Text Available Purpose. We quantify the additional radiation exposure in terms of effective dose incurred by patients in the CT portion of SPECT/CT examinations. Methods. The effective dose from a variety of common nuclear medicine procedures is calculated and summarized. The extra exposure from the CT portion of the examination is summarized by examination and body part. Two hundred forty-eight scans from 221 patients are included in this study. The effective dose from the CT examination is also compared to average background radiation. Results. We found that the extra effective dose is not sufficient to cause deterministic effects. However, the stochastic effects may be significant, especially in patients undergoing numerous follow-up studies. The cumulative effect might increase the radiation exposure compared to patient management with SPECT alone. Conclusions. While the relative increase in radiation exposure associated with SPECT/CT is generally considered acceptable when compared with the benefits to the patient, physicians should make every effort to minimize this effect by using proper technical procedures and educating patients about the exposure they will receive.

  7. ATP, IMP, and glycogen in cod muscle at onset and during development of rigor mortis depend on the sampling location

    DEFF Research Database (Denmark)

    Cappeln, Gertrud; Jessen, Flemming

    2002-01-01

    Variation in glycogen, ATP, and IMP contents within individual cod muscles were studied in ice stored fish during the progress of rigor mortis. Rigor index was determined before muscle samples for chemical analyzes were taken at 16 different positions on the fish. During development of rigor, the...

  8. Mindful Parenting Assessed Further : Psychometric Properties of the Dutch Version of the Interpersonal Mindfulness in Parenting Scale (IM-P)

    NARCIS (Netherlands)

    de Bruin, Esther I.; Zijlstra, Bonne J H; Geurtzen, Naline; van Zundert, Rinka M P; van de Weijer-Bergsma, Eva; Hartman, Esther E.; Nieuwesteeg, Anke M.; Duncan, Larissa G.; Bögels, Susan M.

    2014-01-01

    Psychometric properties of the Dutch version of the Interpersonal Mindfulness in Parenting Scale (IM-P) were studied in a general population sample of mothers of adolescents (n = 866) (study 1). A six-factor structure (29 items) emerged using exploratory factor analysis. A main difference from the o

  9. Mindful Parenting Assessed Further: Psychometric Properties of the Dutch Version of the Interpersonal Mindfulness in Parenting Scale (IM-P)

    NARCIS (Netherlands)

    Bruin, E.I. de; Zijlstra, B.J.H.; Geurtzen, N.; Zundert, R.M.P. van; Weijer-Bergsma, E. van de; Hartman, E.E.; Nieuwesteeg, A.M.; Duncan, L.G.; Bögels, S.M.

    2014-01-01

    Psychometric properties of the Dutch version of the Interpersonal Mindfulness in Parenting Scale (IM-P) were studied in a general population sample of mothers of adolescents (nn=n866) (study 1). A six-factor structure (29 items) emerged using exploratory factor analysis. A main difference from the o

  10. Mindful parenting assessed further: psychometric properties of the Dutch version of the Interpersonal Mindfulness in Parenting Scale (IM-P)

    NARCIS (Netherlands)

    de Bruin, E.I.; Zijlstra, B.J.H.; Geurtzen, N.; van Zundert, R.M.P.; van de Weijer-Bergsma, E.; Hartman, E.E.; Nieuwesteeg, A.M.; Duncan, L.G.; Bögels, S.M.

    2014-01-01

    Psychometric properties of the Dutch version of the Interpersonal Mindfulness in Parenting Scale (IM-P) were studied in a general population sample of mothers of adolescents (n = 866) (study 1). A six-factor structure (29 items) emerged using exploratory factor analysis. A main difference from the o

  11. Diseminación de blaIMP-8 en enterobacterias aisladas en un hospital de Buenos Aires

    Directory of Open Access Journals (Sweden)

    Ana M Togneri

    Full Text Available Entre agosto de 2008 y diciembre de 2011 se detectaron en el Hospital Interzonal General de Agudos «Evita» de Lanús 6 aislamientos de enterobacterias productoras de metalo-ß-lactamasas, distribuidos en tres especies: Enterobacter cloacae (4, Klebsiella oxytoca (1 y Citrobacter freundii (1. Los seis aislamientos presentaron un perfil de multirresistencia y se confirmó la presencia del gen blaIMP-8. Cinco aislamientos además expresaron la ß-lactamasa de espectro extendido PER-2. El gen blaIMP-8 fue hallado como primer casete de un integrón de clase 1. Sin embargo, la secuencia 3´ conservada no pudo detectarse en tres aislamientos. En todos los casos, el gen blaIMP-8 fue transferido por conjugación a Escherichia coli resistente a azida. Mediante PFGE se observó que los cuatro aislamientos de E. cloacae no estuvieron genéticamente relacionados. Estos son los primeros hallazgos de metalo-ß-lactamasas en la institución, que sugieren una posible diseminación horizontal del gen blaIMP-8 intra e interespecies.

  12. Mindful parenting assessed further: psychometric properties of the Dutch version of the Interpersonal Mindfulness in Parenting Scale (IM-P)

    NARCIS (Netherlands)

    de Bruin, E.I.; Zijlstra, B.J.H.; Geurtzen, N.; van Zundert, R.M.P.; van de Weijer-Bergsma, E.; Hartman, E.E.; Nieuwesteeg, A.M.; Duncan, L.G.; Bögels, S.M.

    2014-01-01

    Psychometric properties of the Dutch version of the Interpersonal Mindfulness in Parenting Scale (IM-P) were studied in a general population sample of mothers of adolescents (n = 866) (study 1). A six-factor structure (29 items) emerged using exploratory factor analysis. A main difference from the

  13. Mindful Parenting Assessed Further : Psychometric Properties of the Dutch Version of the Interpersonal Mindfulness in Parenting Scale (IM-P)

    NARCIS (Netherlands)

    de Bruin, Esther I.; Zijlstra, Bonne J H; Geurtzen, Naline; van Zundert, Rinka M P; van de Weijer-Bergsma, Eva; Hartman, Esther E.; Nieuwesteeg, Anke M.; Duncan, Larissa G.; Bögels, Susan M.

    Psychometric properties of the Dutch version of the Interpersonal Mindfulness in Parenting Scale (IM-P) were studied in a general population sample of mothers of adolescents (n = 866) (study 1). A six-factor structure (29 items) emerged using exploratory factor analysis. A main difference from the

  14. Mindful Parenting Assessed Further: Psychometric Properties of the Dutch Version of the Interpersonal Mindfulness in Parenting Scale (IM-P)

    NARCIS (Netherlands)

    Bruin, E.I. de; Zijlstra, B.J.H.; Geurtzen, N.; Zundert, R.M.P. van; Weijer-Bergsma, E. van de; Hartman, E.E.; Nieuwesteeg, A.M.; Duncan, L.G.; Bögels, S.M.

    2014-01-01

    Psychometric properties of the Dutch version of the Interpersonal Mindfulness in Parenting Scale (IM-P) were studied in a general population sample of mothers of adolescents (nn=n866) (study 1). A six-factor structure (29 items) emerged using exploratory factor analysis. A main difference from the

  15. Mindful parenting assessed further : Psychometric properties of the Dutch version of the Interpersonal Mindfulness in Parenting Scale (IM-P)

    NARCIS (Netherlands)

    de Bruin, E.I.; Zijlstra, B.J.H.; Geurtzen, N.; van Zundert, R.M.P.; van de Weijer-Bergsma, E.; Hartman, E.E.; Nieuwesteeg, A.M.; Duncan, L.G.; Bögels, S.M.

    2014-01-01

    Psychometric properties of the Dutch version of the Interpersonal Mindfulness in Parenting Scale (IM-P) were studied in a general population sample of mothers of adolescents (n = 866) (study 1). A six-factor structure (29 items) emerged using exploratory factor analysis. A main difference from the

  16. Metabotropic glutamate receptors are involved in the detection of IMP and L-amino acids by mouse taste sensory cells.

    Science.gov (United States)

    Pal Choudhuri, S; Delay, R J; Delay, E R

    2016-03-01

    G-protein-coupled receptors are thought to be involved in the detection of umami and L-amino acid taste. These include the heterodimer taste receptor type 1 member 1 (T1r1)+taste receptor type 1 member 3 (T1r3), taste and brain variants of mGluR4 and mGluR1, and calcium sensors. While several studies suggest T1r1+T1r3 is a broadly tuned lLamino acid receptor, little is known about the function of metabotropic glutamate receptors (mGluRs) in L-amino acid taste transduction. Calcium imaging of isolated taste sensory cells (TSCs) of T1r3-GFP and T1r3 knock-out (T1r3 KO) mice was performed using the ratiometric dye Fura 2 AM to investigate the role of different mGluRs in detecting various L-amino acids and inosine 5' monophosphate (IMP). Using agonists selective for various mGluRs such as (RS)-3,5-dihydroxyphenylglycine (DHPG) (an mGluR1 agonist) and L-(+)-2-amino-4-phosphonobutyric acid (l-AP4) (an mGluR4 agonist), we evaluated TSCs to determine if they might respond to these agonists, IMP, and three L-amino acids (monopotassium L-glutamate, L-serine and L-arginine). Additionally, we used selective antagonists against different mGluRs such as (RS)-L-aminoindan-1,5-dicarboxylic acid (AIDA) (an mGluR1 antagonist), and (RS)-α-methylserine-O-phosphate (MSOP) (an mGluR4 antagonist) to determine if they can block responses elicited by these L-amino acids and IMP. We found that L-amino acid- and IMP-responsive cells also responded to each agonist. Antagonists for mGluR4 and mGluR1 significantly blocked the responses elicited by IMP and each of the L-amino acids. Collectively, these data provide evidence for the involvement of taste and brain variants of mGluR1 and mGluR4 in L-amino acid and IMP taste responses in mice, and support the concept that multiple receptors contribute to IMP and L-amino acid taste.

  17. Determination of the Ionic Charge States of SEPs Using the University of Chicago IMP-8 Instrument

    Science.gov (United States)

    Dietrich, W.

    We use a new method to calculate the mean ionization charge state of solar energetic particles (SEPs) observed with the University of Chicago Cosmic Ray Nuclear Composition experiment on the IMP-8 satellite. The method, using the time to maximum flux, is demonstrated for several gradual SEP events, including the events on 29 September 1989, 19 October 1989, 24 October 1989, and 6 November 1997. Mean ionic charge states are deduced for heavy ions with energies in the range ~10- 500 MeV/nucleon. The ionic charge determination is made only during the onset of the SEP events. These mean charge states agree well with previous measurements for SEP events both at low energy (~0.5-4 MeV/nucleon reported by ISEE-3) and at higher energies (~200-500 MeV/nucleon reported by LDEF). The mean ionic charge states are then used to determine an average temperature and source region for these particles.

  18. Base-ball: imposition impériale ou affirmation du sentiment national?

    Directory of Open Access Journals (Sweden)

    Elizabeth Burgos

    2006-05-01

    Full Text Available Dans un livre consacré à la figure de l’actuel président du Venezuela, Hugo Chávez, le journaliste britannique Richard Gott, spécialiste reconnu de l’Amérique Latine, exprime un grand étonnement au sujet de la passion que les deux paladins de l’anti-impérialisme, Fidel Castro et Hugo Chávez, partagent avec les Etats-Unis. Mais pour le journaliste britannique les choses ne s’arrêtent pas là car il constate que cette passion – qu’il considère comme un « accident de l’histoire » - est également ...

  19. Influence of the environment and phototoxicity of the live cell imaging system at IMP microbeam facility

    Science.gov (United States)

    Liu, Wenjing; Du, Guanghua; Guo, Jinlong; Wu, Ruqun; Wei, Junzhe; Chen, Hao; Li, Yaning; Zhao, Jing; Li, Xiaoyue

    2017-08-01

    To investigate the spatiotemporal dynamics of DNA damage and repair after the ion irradiation, an online live cell imaging system has been established based on the microbeam facility at Institute of Modern Physics (IMP). The system could provide a sterile and physiological environment by making use of heating plate and live cell imaging solution. The phototoxicity was investigated through the evaluation of DNA repair protein XRCC1 foci formed in HT1080-RFP cells during the imaging exposure. The intensity of the foci induced by phototoxicity was much lower compared with that of the foci induced by heavy ion hits. The results showed that although spontaneous foci were formed due to RFP exposure during live cell imaging, they had little impact on the analysis of the recruitment kinetics of XRCC1 in the foci induced by the ion irradiation.

  20. La marine impériale dans l’expédition de Chine

    OpenAIRE

    histoire, Jiang TianyueDoctorant en; Duplaix, Université normale de Pékin. Article corrigé et condensé par Alexandre Sheldon-; SHD., chercheur au

    2014-01-01

    L’auteur étudie le développement de la présence navale française dans les mers de Chine durant les guerres de l’opium. Si le soutien aux missions lui apparaît comme un prétexte pour ne pas laisser la Grande-Bretagne gagner seule des avantages en Chine, la marine impériale est la condition du succès de l’intervention. The author examines the development of the French naval presence in the seas of China during the Opium Wars. If supporting the [religious] missions seems an excuse not to let ...

  1. Santos e devotos no império ultramarino português

    Directory of Open Access Journals (Sweden)

    Beatriz Catão Cruz Santos

    2009-01-01

    Full Text Available O artigo faz uma história da coleção de vilancicos de São Gonçalo de Amarante, que integram a Coleção Barbosa Machado na Biblioteca Nacional do Rio de Janeiro e, simultaneamente, apresenta reflexões sobre a relação santo-devoto no Império Ultramarino Português no século XVIII. Os vilancicos são identificados como um gênero retórico poético que evoca o santo e uma prática religiosa que contrastava com outras. Parte-se de uma perspectiva comparativa entre os vilancicos e outras evidências sobre a relação santo-devoto e, em particular, sobre o santo e sua festa na sociedade colonial escravista. O artigo dialoga com a historiografia centrada na noção de Império e com uma bibliografia da História e das Ciências Sociais voltada para a relação santodevoto, a santidade e as festas.The paper produces a story of vilancicos for Saint Gonçalo do Amarante, that are part of the Rio de Janeiro National Library Barbosa Machado Collection and presents reflections on the relation saint-devotees in the Portuguese Overseas Empire during the 18th century. The vilancicos are identified as a rhetoric poetic genre that reminds the saint and a religious practice that contrasted with others. It starts from a comparative perspective between the vilancicos and other evidences about the relation saint-devotee, and in particular, about the saint and his feast in a colonial and proslavery society. The article deals with the historiography centered in the notion of Empire, and with a bibliography of History and Social Sciences pointed to the relation saint-devotee, the holiness and the feasts.

  2. Ilustração e império colonial Ilustration and colonial empire

    Directory of Open Access Journals (Sweden)

    Ronald Raminelli

    2012-12-01

    Full Text Available Entre 1780 e 1808, as principais reflexões sobre o império colonial lusitano partiam de colonos e de reinóis radicados no Brasil. A perspectiva luso-brasileira vinculava-se tanto à formação na Universidade de Coimbra quanto à vivência na América portuguesa. Pensar o império, nessa conjuntura, era analisar a centralidade de Lisboa e do Brasil, avaliar os nexos entre o reino, as terras brasílicas e as demais possessões na África. Para os ilustrados, aos poucos, o Brasil tornava-se um centro para os domínios lusitanos em Angola e Moçambique. Aliás, muito antes da vinda corte, as terras lusas na América já ganhavam centralidade na produção visual e escrita dos súditos de Sua Majestade.Between 1780 and 1808, the main reflections on the colonial Portuguese empire came from settlers and reinois living in Brazil. The Portuguese-Brazilian prospect was linked to both training at the University of Coimbra and living experience in Portuguese America. Thinking about the empire, at this juncture, used to be associated to examine the centrality of Lisbon and Brazil, to evaluate the links between the kingdom, the Brazilian lands and other possessions in Africa. Slowly, for those illustrated, Brazil became a center for Portuguese domains in Angola and Mozambique. Indeed, long before the Portuguese court arrived in Americ, ita has gained centrality in the production of written and visual subjects of His Majesty.

  3. The additive prognostic value of perfusion and functional data assessed by quantitative gated SPECT in women

    NARCIS (Netherlands)

    Y.G.C.J. America (Yves); J.J. Bax (Jeroen); H. Boersma (Eric); M. Stokkel (Marcel); E.E. van der Wall (Ernst)

    2009-01-01

    textabstractBackground: The aim of this study was to assess the prognostic value of technetium-99m tetrofosmin gated SPECT imaging in women using quantitative gated single photon emission computed tomography (SPECT) imaging. Methods: We followed 453 consecutive female patients. Average follow-up was

  4. Evaluation of the use of freehand SPECT for sentinel node biopsy in early stage oral carcinoma

    NARCIS (Netherlands)

    Heuveling, D.A.; van Weert, S.; Karagozoglu, K.H.; de Bree, R.

    2015-01-01

    Rationale Inadequate intraoperative visualization of the sentinel node can hamper its harvest. Freehand SPECT is a 3D tomographic imaging modality based on the concepts of SPECT, which can be used for intraoperative visualization and navigation towards the sentinel node in order to improve its

  5. SPECT-OPT multimodal imaging enables accurate evaluation of radiotracers for beta-cell mass assessments

    NARCIS (Netherlands)

    Eter, W.A.; Parween, S.; Joosten, L.; Frielink, C.; Eriksson, M.; Brom, M.; Ahlgren, U.; Gotthardt, M.

    2016-01-01

    Single Photon Emission Computed Tomography (SPECT) has become a promising experimental approach to monitor changes in beta-cell mass (BCM) during diabetes progression. SPECT imaging of pancreatic islets is most commonly cross-validated by stereological analysis of histological pancreatic sections

  6. GATE simulation of a new design of pinhole SPECT system for small animal brain imaging

    Science.gov (United States)

    Uzun Ozsahin, D.; Bläckberg, L.; El Fakhri, G.; Sabet, H.

    2017-01-01

    Small animal SPECT imaging has gained an increased interest over the past decade since it is an excellent tool for developing new drugs and tracers. Therefore, there is a huge effort on the development of cost-effective SPECT detectors with high capabilities. The aim of this study is to simulate the performance characteristics of new designs for a cost effective, stationary SPECT system dedicated to small animal imaging with a focus on mice brain. The conceptual design of this SPECT system platform, Stationary Small Animal SSA-SPECT, is to use many pixelated CsI:TI detector modules with 0.4 mm × 0.4 mm pixels in order to achieve excellent intrinsic detector resolution where each module is backed by a single pinhole collimator with 0.3 mm hole diameter. In this work, we present the simulation results of four variations of the SSA-SPECT platform where the number of detector modules and FOV size is varied while keeping the detector size and collimator hole size constant. Using the NEMA NU-4 protocol, we performed spatial resolution, sensitivity, image quality simulations followed by a Derenzo-like phantom evaluation. The results suggest that all four SSA-SPECT systems can provide better than 0.063% system sensitivity and < 1.5 mm FWHM spatial resolution without resolution recovery or other correction techniques. Specifically, SSA-SPECT-1 showed a system sensitivity of 0.09% in combination with 1.1 mm FWHM spatial resolution.

  7. Quantitative multi-pinhole small-animal SPECT : uniform versus non-uniform Chang attenuation correction

    NARCIS (Netherlands)

    Wu, C.; de Jong, J. R.; van Andel, H. A. Gratama; van der Have, F.; Vastenhouw, B.; Laverman, P.; Boerman, O. C.; Dierckx, R. A. J. O.; Beekman, F. J.

    2011-01-01

    Attenuation of photon flux on trajectories between the source and pinhole apertures affects the quantitative accuracy of reconstructed single-photon emission computed tomography (SPECT) images. We propose a Chang-based non-uniform attenuation correction (NUA-CT) for small-animal SPECT/CT with focusi

  8. Quantitative multi-pinhole small-animal SPECT: uniform versus non-uniform Chang attenuation correction

    NARCIS (Netherlands)

    Wu, C.; Jong, J.R. de; Gratama van Andel, H.A.; Have, F. van der; Vastenhouw, B.; Laverman, P.; Boerman, O.C.; Dierckx, R.A.; Beekman, F.J.

    2011-01-01

    Attenuation of photon flux on trajectories between the source and pinhole apertures affects the quantitative accuracy of reconstructed single-photon emission computed tomography (SPECT) images. We propose a Chang-based non-uniform attenuation correction (NUA-CT) for small-animal SPECT/CT with focusi

  9. Implementation of angular response function modeling in SPECT simulations with GATE

    Energy Technology Data Exchange (ETDEWEB)

    Descourt, P; Visvikis, D [INSERM, U650, LaTIM, IFR SclnBioS, Universite de Brest, CHU Brest, Brest, F-29200 (France); Carlier, T; Bardies, M [CRCNA INSERM U892, Nantes (France); Du, Y; Song, X; Frey, E C; Tsui, B M W [Department of Radiology, J Hopkins University, Baltimore, MD (United States); Buvat, I, E-mail: dimitris@univ-brest.f [IMNC-UMR 8165 CNRS Universites Paris 7 et Paris 11, Orsay (France)

    2010-05-07

    Among Monte Carlo simulation codes in medical imaging, the GATE simulation platform is widely used today given its flexibility and accuracy, despite long run times, which in SPECT simulations are mostly spent in tracking photons through the collimators. In this work, a tabulated model of the collimator/detector response was implemented within the GATE framework to significantly reduce the simulation times in SPECT. This implementation uses the angular response function (ARF) model. The performance of the implemented ARF approach has been compared to standard SPECT GATE simulations in terms of the ARF tables' accuracy, overall SPECT system performance and run times. Considering the simulation of the Siemens Symbia T SPECT system using high-energy collimators, differences of less than 1% were measured between the ARF-based and the standard GATE-based simulations, while considering the same noise level in the projections, acceleration factors of up to 180 were obtained when simulating a planar 364 keV source seen with the same SPECT system. The ARF-based and the standard GATE simulation results also agreed very well when considering a four-head SPECT simulation of a realistic Jaszczak phantom filled with iodine-131, with a resulting acceleration factor of 100. In conclusion, the implementation of an ARF-based model of collimator/detector response for SPECT simulations within GATE significantly reduces the simulation run times without compromising accuracy. (note)

  10. SPECT assay of radiolabeled monoclonal antibodies. Final performance report, March 1992--November 1995

    Energy Technology Data Exchange (ETDEWEB)

    Jaszczak, R.J.

    1995-12-01

    Research is described in the following areas: development and evaluation quantitatively of reconstruction algorithms with improved compensations for attenuation, scatter, and geometric collimator response; evaluation of single photon emission computed tomography (SPECT) quantification of iodine 123 and astatine 211; and the development and evaluation of SPECT pinhole imaging for low and medium energy photons.

  11. NOTE: Implementation of angular response function modeling in SPECT simulations with GATE

    Science.gov (United States)

    Descourt, P.; Carlier, T.; Du, Y.; Song, X.; Buvat, I.; Frey, E. C.; Bardies, M.; Tsui, B. M. W.; Visvikis, D.

    2010-05-01

    Among Monte Carlo simulation codes in medical imaging, the GATE simulation platform is widely used today given its flexibility and accuracy, despite long run times, which in SPECT simulations are mostly spent in tracking photons through the collimators. In this work, a tabulated model of the collimator/detector response was implemented within the GATE framework to significantly reduce the simulation times in SPECT. This implementation uses the angular response function (ARF) model. The performance of the implemented ARF approach has been compared to standard SPECT GATE simulations in terms of the ARF tables' accuracy, overall SPECT system performance and run times. Considering the simulation of the Siemens Symbia T SPECT system using high-energy collimators, differences of less than 1% were measured between the ARF-based and the standard GATE-based simulations, while considering the same noise level in the projections, acceleration factors of up to 180 were obtained when simulating a planar 364 keV source seen with the same SPECT system. The ARF-based and the standard GATE simulation results also agreed very well when considering a four-head SPECT simulation of a realistic Jaszczak phantom filled with iodine-131, with a resulting acceleration factor of 100. In conclusion, the implementation of an ARF-based model of collimator/detector response for SPECT simulations within GATE significantly reduces the simulation run times without compromising accuracy.

  12. High-resolution tomography of positron emitters with clustered pinhole SPECT

    NARCIS (Netherlands)

    Goorden, M.C.; Beekman, F.J.

    2010-01-01

    State-of-the-art small-animal single photon emission computed tomography (SPECT) enables sub-half-mm resolution imaging of radio-labelled molecules. Due to severe photon penetration through pinhole edges, current multi-pinhole SPECT is not suitable for high-resolution imaging of photons with high

  13. Evaluation of quantitative accuracy in CZT-based pre-clinical SPECT for various isotopes

    Science.gov (United States)

    Park, S.-J.; Yu, A. R.; Kim, Y.-s.; Kang, W.-S.; Jin, S. S.; Kim, J.-S.; Son, T. J.; Kim, H.-J.

    2015-05-01

    In vivo pre-clinical single-photon emission computed tomography (SPECT) is a valuable tool for functional small animal imaging, but several physical factors, such as scatter radiation, limit the quantitative accuracy of conventional scintillation crystal-based SPECT. Semiconductor detectors such as CZT overcome these deficiencies through superior energy resolution. To our knowledge, little scientific information exists regarding the accuracy of quantitative analysis in CZT-based pre-clinical SPECT systems for different isotopes. The aim of this study was to assess the quantitative accuracy of CZT-based pre-clinical SPECT for four isotopes: 201Tl, 99mTc, 123I, and 111In. The quantitative accuracy of the CZT-based Triumph X-SPECT (Gamma-Medica Ideas, Northridge, CA, U.S.A.) was compared with that of a conventional SPECT using GATE simulation. Quantitative errors due to the attenuation and scatter effects were evaluated for all four isotopes with energy windows of 5%, 10%, and 20%. A spherical source containing the isotope was placed at the center of the air-or-water-filled mouse-sized cylinder phantom. The CZT-based pre-clinical SPECT was more accurate than the conventional SPECT. For example, in the conventional SPECT with an energy window of 10%, scatter effects degraded quantitative accuracy by up to 11.52%, 5.10%, 2.88%, and 1.84% for 201Tl, 99mTc, 123I, and 111In, respectively. However, with the CZT-based pre-clinical SPECT, the degradations were only 9.67%, 5.45%, 2.36%, and 1.24% for 201Tl, 99mTc, 123I, and 111In, respectively. As the energy window was increased, the quantitative errors increased in both SPECT systems. Additionally, the isotopes with lower energy of photon emissions had greater quantitative error. Our results demonstrated that the CZT-based pre-clinical SPECT had lower overall quantitative errors due to reduced scatter and high detection efficiency. Furthermore, the results of this systematic assessment quantifying the accuracy of these SPECT

  14. Insulin-like growth factor II mRNA binding protein 3 (IMP3 is overexpressed in prostate cancer and correlates with higher Gleason scores

    Directory of Open Access Journals (Sweden)

    Mortezavi Ashkan

    2010-06-01

    Full Text Available Abstract Background The oncofetal protein insulin-like growth factor II mRNA binding protein 3 (IMP3 is an important factor for cell-migration and adhesion in malignancies. Recent studies have shown a remarkable overexpression of IMP3 in different human malignant neoplasms and also revealed it as an important prognostic marker in some tumor entities. To our knowledge, IMP3 expression has not been investigated in prostate carcinomas so far. Methods Immunohistochemical stainings for IMP3 were performed on tissue microarray (TMA organized samples from 507 patients: 31 normal prostate tissues, 425 primary carcinomas and 51 prostate cancer metastases or castration-resistant prostate cancers (CRPC. IMP3 immunoreactivity was semiquantitatively scored and correlated with clinical-pathologic parameters including survival. Results IMP3 is significantly stronger expressed in prostate carcinomas compared to normal prostate tissues (p Conclusions Although IMP3 is overexpressed in a significant proportion of prostate cancer cases, which might be of importance for novel therapeutic approaches, it does not appear to possess any immediate diagnostic or prognostic value, limiting its potential as a tissue biomarker for prostate cancer. These results might be corroborated by the fact, that two independent tumor cohorts were separately reviewed.

  15. Interictal brain SPECT in patients with medically refractory temporal lobe epilepsy; SPECT cerebral interictal em pacientes com epilepsia do lobo temporal de dificil controle

    Energy Technology Data Exchange (ETDEWEB)

    Andraus, Maria Emilia Cosenza

    2000-06-01

    The brain single photon emission computed tomography (SPECT) is s functional neuroimaging method that can detect localized changes in cerebral blood flow. The temporal lobe epilepsy (TLE) is the most common epileptic syndrome in adults, and more than 50% are medically refractory. The SPECT can contribute to investigation of epileptogenic focus and is one of the methods of pre-surgical evaluation of these patients. (author)

  16. Investigating the role of SPECT/CT in dynamic sentinel lymph node biopsy for penile cancers

    Energy Technology Data Exchange (ETDEWEB)

    Saad, Ziauddin Zia; Bomanji, Jamshed [University College Hospitals London, Department of Nuclear Medicine, London (United Kingdom); UCLH NHS Foundation Trust, Institute of Nuclear Medicine, 5th Floor, London (United Kingdom); Omorphos, Savvas; Malone, Peter; Nigam, Raj; Muneer, Asif [University College Hospitals London, Department of Urology, London (United Kingdom); Michopoulou, Sofia; Gacinovic, Svetislav [University College Hospitals London, Department of Nuclear Medicine, London (United Kingdom)

    2017-07-15

    Currently, most centres use 2-D planar lymphoscintigraphy when performing dynamic sentinel lymph node biopsy in penile cancer patients with clinically impalpable inguinal nodes. This study aimed to investigate the role of SPECT/CT following 2-D planar lymphoscintigraphy (dynamic and static) in the detection and localization of sentinel lymph nodes in the groin. A qualitative (visual) review was performed on planar followed by SPECT/CT lymphoscintigraphy in 115 consecutive patients (age 28-86 years) who underwent injection of {sup 99m}Tc-nanocolloid followed by immediate acquisition of dynamic (20 min) and early static scans (5 min) initially and further delayed static (5 min) images at 120 min followed by SPECT/CT imaging. The lymph nodes detected in each groin on planar lymphoscintigraphy and SPECT/CT were compared. A total of 440 and 467 nodes were identified on planar scintigraphy and SPECT/CT, respectively. Overall, SPECT/CT confirmed the findings of planar imaging in 28/115 cases (24%). In the remaining 87 cases (76%), gross discrepancies were observed between planar and SPECT/CT images. SPECT/CT identified 17 instances of skin contamination (16 patients, 13%) and 36 instances of in-transit lymphatic tract activity (24 patients, 20%) that had been interpreted as tracer-avid lymph nodes on planar imaging. In addition, SPECT/CT identified 53 tracer-avid nodes in 48 patients (42%) that were not visualized on planar imaging and led to reclassification of the drainage basins (pelvic/inguinal) of 27 tracer-avid nodes. The addition of SPECT/CT improved the rate of detection of true tracer-avid lymph nodes and delineated their precise (3-D) anatomic localization in drainage basins. (orig.)

  17. Effects of RNAi-Mediated Knockdown of Histone Methyltransferases on the Sex-Specific mRNA Expression of Imp in the Silkworm Bombyx mori

    Directory of Open Access Journals (Sweden)

    Masataka G. Suzuki

    2014-04-01

    Full Text Available Sexual differentiation in Bombyx mori is controlled by sex-specific splicing of Bmdsx, which results in the omission of exons 3 and 4 in a male-specific manner. In B. mori, insulin-like growth factor II mRNA-binding protein (Imp is a male-specific factor involved in male-specific splicing of Bmdsx. Male-specific Imp mRNA results from the male-specific inclusion of exon 8. To verify the link between histone methylation and alternative RNA processing in Imp, we examined the effects of RNAi-mediated knockdown of several histone methyltransferases on the sex-specific mRNA expression of Imp. As a result, male-specific expression of Imp mRNA was completely abolished when expression of the H3K79 methyltransferase DOT1L was repressed to <10% of that in control males. Chromatin immunoprecipitation-quantitative PCR analysis revealed a higher distribution of H3K79me2 in normal males than in normal females across Imp. RNA polymerase II (RNAP II processivity assays indicated that RNAi knockdown of DOT1L in males caused a twofold decrease in RNAP II processivity compared to that in control males, with almost equivalent levels to those observed in normal females. Inhibition of RNAP II-mediated elongation in male cells repressed the male-specific splicing of Imp. Our data suggest the possibility that H3K79me2 accumulation along Imp is associated with the male-specific alternative processing of Imp mRNA that results from increased RNAP II processivity.

  18. The novel anthraquinone derivative IMP1338 induces death of human cancer cells by p53-independent S and G2/M cell cycle arrest.

    Science.gov (United States)

    Choi, Hyun Kyung; Ryu, Hwani; Son, A-Rang; Seo, Bitna; Hwang, Sang-Gu; Song, Jie-Young; Ahn, Jiyeon

    2016-04-01

    To identify novel small molecules that induce selective cancer cell death, we screened a chemical library containing 1040 compounds in HT29 colon cancer and CCD18-Co normal colon cells, using a phenotypic cell-based viability assay system with the Cell Counting Kit-8 (CCK-8). We discovered a novel anthraquinone derivative, N-(4-[{(9,10-dioxo-9,10-dihydro-1-anthracenyl)sulfonyl}amino]phenyl)-N-methylacetamide (IMP1338), which was cytotoxic against the human colon cancer cells tested. The MTT cell viability assay showed that treatment with IMP1338 selectively inhibited HCT116, HCT116 p53(-/-), HT29, and A549 cancer cell proliferation compared to that of Beas2B normal epithelial cells. To elucidate the cellular mechanism underlying the cytotoxicity of IMP1338, we examined the effect of IMP1338 on the cell cycle distribution and death of cancer cells. IMP1338 treatment significantly arrested the cell cycle at S and G2/M phases by DNA damage and led to apoptotic cell death, which was determined using FACS analysis with Annexin V/PI double staining. Furthermore, IMP1338 increased caspase-3 cleavage in wild-type p53, p53 knockout HCT116, and HT29 cells as determined using immunoblotting. In addition, IMP1338 markedly induced the phosphorylation of histone H2AX and Chk1 in both cell lines while the combination of 5-fluorouracil (5-FU) and radiation inhibited the viability of HCT116, HCT116 p53(-/-), and HT29 cells compared to 5-FU or radiation alone. Our findings indicated that IMP1338 induced p53-independent cell death through S and G2/M phase arrest as well as DNA damage. These results provide a basis for future investigations assessing the promising anticancer properties of IMP1338.

  19. The role of Sesta-Mibi-SPECT in angiographically documented coronary heart disease. Zur Bedeutung der Sesta-Mibi-SPECT bei angiographisch dokumentierter koronarer Herzerkrankung

    Energy Technology Data Exchange (ETDEWEB)

    Vogt, H. (Inst. fuer Nuklearmedizin, 1. Medizinische Klinik, Zentralklinikum Augsburg (Germany)); Graf, G. (Inst. fuer Nuklearmedizin, 1. Medizinische Klinik, Zentralklinikum Augsburg (Germany)); Krawietz, W. (Inst. fuer Nuklearmedizin, 1. Medizinische Klinik, Zentralklinikum Augsburg (Germany)); Bolte, H.D. (Inst. fuer Nuklearmedizin, 1. Medizinische Klinik, Zentralklinikum Augsburg (Germany)); Heidenreich, P. (Inst. fuer Nuklearmedizin, 1. Medizinische Klinik, Zentralklinikum Augsburg (Germany))

    1994-04-01

    131 patients were studied to compare the results of 99mTc-Mibi-SPECT with coronary angiography (CAG). 127 patients remained for final evaluation. A scar was falsely diagnosed in 17. 13 patients had a normal CAG and pathologic Sesta-Mibi-SPECT. 5 of them suffered from cardiomyopathy. 2 patients have had a myocarditis, at 2 others a PTCA was perfomred. 1 patient had a 20% LCA-stenosis and another one a left ventricular hypertrophy. Two times the reason for the pathologic Sesta-Mibi-SPECT could not be found. 1 patient had a normal Sesta-Mibi-SPECT despite of a pathologic CAG, angina pectoris and pathologic ECG. We got congruent results in 113 patients. 7 patients showed a left bundle branch block (LBBB), in 5 of them the Mibi-SPECT corresponded well with the CAG. 2 patients with dilated cardiomyopathy and no CHD showed an exercise induced ischemia in the anteroseptal wall. In patiens with angiographically documented CHD Sesta-Mibi-SPECT gives reliable diagnostic information concerning the myocardial perfusion. (orig./MG)

  20. mTOR complex 2 phosphorylates IMP1 cotranslationally to promote IGF2 production and the proliferation of mouse embryonic fibroblasts

    DEFF Research Database (Denmark)

    Dai, Ning; Christiansen, Jan; Nielsen, Finn;

    2013-01-01

    phosphorylation at Ser181, which is catalyzed cotranslationally by mTOR complex 2 (mTORC2). Phosphorylation strongly enhances IMP1 binding to the IGF2-leader 3 5' untranslated region, which is absolutely required to enable IGF2-leader 3 mRNA translational initiation by internal ribosomal entry. These findings...... uncover a new mechanism by which mTOR regulates organismal growth by promoting IGF2 production in the mouse embryo through mTORC2-catalyzed cotranslational IMP1/IMP3 phosphorylation. Inasmuch as TORC2 is activated by association with ribosomes, the present results indicate that mTORC2-catalyzed...

  1. Impact of reconstruction parameters on quantitative I-131 SPECT

    Science.gov (United States)

    van Gils, C. A. J.; Beijst, C.; van Rooij, R.; de Jong, H. W. A. M.

    2016-07-01

    Radioiodine therapy using I-131 is widely used for treatment of thyroid disease or neuroendocrine tumors. Monitoring treatment by accurate dosimetry requires quantitative imaging. The high energy photons however render quantitative SPECT reconstruction challenging, potentially requiring accurate correction for scatter and collimator effects. The goal of this work is to assess the effectiveness of various correction methods on these effects using phantom studies. A SPECT/CT acquisition of the NEMA IEC body phantom was performed. Images were reconstructed using the following parameters: (1) without scatter correction, (2) with triple energy window (TEW) scatter correction and (3) with Monte Carlo-based scatter correction. For modelling the collimator-detector response (CDR), both (a) geometric Gaussian CDRs as well as (b) Monte Carlo simulated CDRs were compared. Quantitative accuracy, contrast to noise ratios and recovery coefficients were calculated, as well as the background variability and the residual count error in the lung insert. The Monte Carlo scatter corrected reconstruction method was shown to be intrinsically quantitative, requiring no experimentally acquired calibration factor. It resulted in a more accurate quantification of the background compartment activity density compared with TEW or no scatter correction. The quantification error relative to a dose calibrator derived measurement was found to be  Gaussian or no CDR modelling. Scatter correction showed a small effect on quantification of small volumes. When using a weighting factor, TEW correction was comparable to Monte Carlo reconstruction in all measured parameters, although this approach is clinically impractical since this factor may be patient dependent. Monte Carlo based scatter correction including accurately simulated CDR modelling is the most robust and reliable method to reconstruct accurate quantitative iodine-131 SPECT images.

  2. Impact of subcortical white matter lesions on dopamine transporter SPECT.

    Science.gov (United States)

    Funke, Elisabeth; Kupsch, Andreas; Buchert, Ralph; Brenner, Winfried; Plotkin, Michail

    2013-07-01

    Subcortical arteriosclerotic encephalopathy (SAE) can affect the nigrostriatal system and presumably cause vascular parkinsonism (VP). However, in patients with SAE, the differentiation of VP from idiopathic Parkinson's disease (IPS) is challenging. The aim of the present study was to examine the striatal dopamine transporter (DAT) density in patients with parkinsonism and SAE. Fifteen consecutive patients with parkinsonian symptoms displayed SAE, as detected by magnetic resonance imaging (MRI). Fifteen retrospectively chosen, matched patients with diagnosis of IPS without any abnormalities in MRI served as a reference group. DAT SPECT was performed using the tracer ¹²³I-FP-CIT. Scans were acquired on a triple-head SPECT system (Multispect 3, Siemens) and analysed using the investigator-independent BRASS™ software (HERMES). In the SAE group, a DAT deficit was observed in 9/15 patients. In contrast, all patients from the IPS group showed a reduced DAT binding (p = 0.008). The specific binding ratios (BR) of putamen contralateral to the side of the more affected limb versus occipital lobe were in trend higher in patients with SAE versus patients in the IPS-group (p = 0.053). Indices for putaminal asymmetry (p = 0.036) and asymmetry caudate-to-putamen (p = 0.026) as well as the ratio caudate-to-putamen (p = 0.048) were significantly higher in IPS patients having no SAE. DAT deficit was less pronounced in patients with SAE and parkinsonism than in patients with IPS without any abnormalities in the MRI. A potential role of DAT SPECT in the differential diagnosis of VP and IPS requires more assessments within prospective studies.

  3. Molecular imaging of cancer using PET and SPECT

    DEFF Research Database (Denmark)

    Kjaer, Andreas

    2006-01-01

    for molecular imaging of cancer. Especially the possibility of a quick transfer of methods developed in animals to patients (translational research) is an important strength. This article will briefly discuss the newest applications and their importance and perspective in relation to the shift in paradigm......Molecular imaging allows for the study of molecular and cellular events in the living intact organism. The nuclear medicine methodologies of positron emission tomography (PET) and single photon emission computer tomography (SPECT) posses several advantages, which make them particularly suited...

  4. Myocardial Perfusion Spect Imaging in Dextrocardia: A Case Report

    Directory of Open Access Journals (Sweden)

    Semra Özdemir

    2013-08-01

    Full Text Available The myocardial perfusion scintigraphy acquisition and analysis present some technical differences in the rare dextrocardia cases. Here we report a case of a 38 year-old woman with dextrocardia who had been applied myocardial perfusion scintigraphy. Presented case showed that the thoracic and abdominal organs had a mirror image with situs inversus totalis type dextrocardia. The incidence of coronary heart disease and life span of people with situs inversus totalis are the same as the normal population. So we may apply myocardial perfusion scintigraphy to this patient group. The current case is presented in order to remind the special applications of myocardial perfusion SPECT imaging in patients with dextrocardia.

  5. The neutron decay spectrometer aSPECT: Latest results

    Energy Technology Data Exchange (ETDEWEB)

    Wirth, Hans-Friedrich; Angerer, Heinz; Konorov, Igor; Petzoldt, Gerd; Simson, Martin; Zimmer, Oliver [Physik-Department, Technische Universtitaet Muenchen (Germany); Ayala Guardia, Fidel; Borg, Michael; Glueck, Ferenc; Heil, Werner; Konrad, Gertrud; Munoz Horta, Raquel; Sobolev, Yury [Institut fuer Physik, Universitaet Mainz (Germany); Baessler, Stefan [Department of Physics, University of Virginia, Charlottesville (United States); Eberhardt, Klaus [Institut fuer Kernchemie, Universitaet Mainz (Germany)

    2008-07-01

    The neutron decay spectrometer aSPECT was designed to measure accurately the proton spectrum of the free neutron decay. The knowledge of the proton spectrum allows to extract the neutrino electron angular correlation coefficient a, from which we will determine with highest accuracy the ratio {lambda}= g{sub A}/g{sub V} of the weak coupling constants of the nucleon. After successful beamtimes in 2005/06 at the FRM-II near Munich we continue the measurements at the ILL in Grenoble. Latest results and experiences with a new proton detector, which is a silicon drift detector, are presented.

  6. High Sensitivity SPECT for Small Animals and Plants

    Energy Technology Data Exchange (ETDEWEB)

    Mitchell, Gregory S. [UC Davis

    2015-02-28

    Imaging systems using single gamma-ray emitting radioisotopes typically implement collimators in order to form the images. However, a tradeoff in sensitivity is inherent in the use of collimators, and modern preclinical single-photon emission computed tomography (SPECT) systems detect a very small fraction of emitted gamma-rays (<0.3%). We have built a collimator-less system, which can reach sensitivity of 40% for 99mTc imaging, while still producing images of sufficient spatial resolution for certain applications in thin objects such as mice, small plants, and well plates used for in vitro experiments.

  7. Robin Hood caught in Wonderland: brain SPECT findings.

    Science.gov (United States)

    Morland, David; Wolff, Valérie; Dietemann, Jean-Louis; Marescaux, Christian; Namer, Izzie Jacques

    2013-12-01

    We present the case of a 53-year-old woman presenting several episodes of body image distortions, ground deformation illusions, and problems assessing distance in the orthostatic position corresponding to the Alice in Wonderland syndrome. No symptoms were reported when sitting or lying down. She had uncontrolled hypertension, hyperglycemia, hypercholesterolemia, and a history of head trauma. Her condition had been diagnosed with left internal carotid artery dissection 2 years earlier. Brain SPECT with 99mTc-ECD performed after i.v. injection of the radiotracer in supine and in standing positions showed hypoperfusion in the healthy contralateral frontoparietal operculum (Robin Hood syndrome), deteriorating when standing up.

  8. Impact of SPECT/CT in imaging inflammation and infection; Wertigkeit der SPECT/CT fuer die nuklearmedizinische Entzuendungsdiagnostik

    Energy Technology Data Exchange (ETDEWEB)

    Linke, R. [Klinikum Bremen-Mitte, Bremen (Germany). Klinik fuer Nuklearmedizin; Kuwert, T. [Erlangen-Nuernberg Univ., Erlangen (Germany). Nuklearmedizinische Klinik mit Poliklinik

    2011-03-15

    Even today infection remains a significant concern, and the diagnosis and localization of infectious foci is an important health issue. As an established infection-imaging modality, nuclear medicine plays a vital health-care role in the diagnosis and subsequent effective treatment of this condition. Several techniques in nuclear medicine significantly aid infection diagnosis, including triple-phase bone scanning, {sup 18}F-FDG-PET and imaging with {sup 111}In-oxine-, {sup 99m}Tc-HMPAO-labeled leukocytes. Each radiopharmaceutical has specific advantages and disadvantages that makes it suitable to diagnose different infectious processes (e.g., soft-tissue sepsis, inflammatory bowel disease, osteomyelitis, occult fever, fever of unknown origin, and infections commonly found in immuno-compromised patients). However, their clinical applications may be limited by the relatively low spatial resolution and the lack of anatomic landmarks of a highly specific tracer with only scarce background uptake to use as a framework for orientation. Anatomic imaging modalities such as CT provide a high-quality assessment of structural abnormalities related to infection, but these structural abnormalities may be unspecific. Furthermore, to detect infection before anatomical changes are present, functional imaging could have some advantages over anatomical imaging. Scintigraphic studies have demonstrated high sensitivity and specificity to an infectious process. Diagnosis and precise delineation of infection may be challenging in certain clinical scenarios, rendering decisions concerning further patient management difficult. The SPECT/CT-technology combines the acquisition of SPECT and CT data with the same imaging device enabling perfect overlay of anatomical and functional images. SPECT/CT imaging data has been shown to be beneficial for many clinical settings such as indeterminate findings in bone scintigraphy, orthopaedic disorders, endocrine, and neuroendocrine tumors. Therefore

  9. MRI and brain spect findings in patients with unilateral temporal lobe epilepsy and normal CT scan

    Directory of Open Access Journals (Sweden)

    P.G. Carrilho

    1994-06-01

    Full Text Available 26 patients with temporal lobe epilepsy clinically documented by several abnormal interictal surface EEGs with typical unitemporal epileptiform activity and a normal CT scan were studied. Interictal99mTC HMPAO brain SPECT and MRI were performed in all subjects. Abnormalities were shown in 61.5% of MRI (n=16 and 65.4% of SPECT (n=17. Hippocampal atrophy associated to a high signal on T2-weighted MRI slices suggesting mesial temporal sclerosis was the main finding (n=12; 75% of abnormal MRI. MRI correlated well to surface EEG in 50% (n=13. There was also a good correlation between MRI and SPECT in 30.7% (n=8. SPECT and EEG were in agreement in 57.7% (n=l5. MRI, SPECT and EEG were congruent in 26.9% (n=7. These results support the usefulness of interictal brain SPECT and MRI in detecting lateralized abnormalities in temporal lobe epilepsy. On the other hand, in two cases, interictal SPECT correlated poorly with surface EEG. This functional method should not be used isolately in the detection of temporal lobe foci. MRI is more useful than CT as a neuroimaging technique in temporal lobe epilepsy. It may detect small structural lesions and mesial temporal lobe sclerosis which are not easily seen with traditional CT scanning.

  10. The usefulness of myocardial SPECT for the preoperative cardiac risk evaluation in noncardiac surgery

    Energy Technology Data Exchange (ETDEWEB)

    Lim, Seok Tae; Lee, Dong Soo; Kang, Won Jon; Chung, June Key; Lee, Myung Chul [College of Medicine, Seoul National Univ., Seoul (Korea, Republic of)

    1999-02-01

    We investigated whether myocardial SPECT had additional usefulness to clinical, functional or surgical indices for the preoperative evaluation of cardiac risks in noncardiac surgery. 118 patients ( M: F=66: 52, 62.7{+-}10.5 years) were studied retrospectively. Eighteen underwent vascular surgeries and 100 nonvascular surgeries. Rest Tl-201/ stress Tc-99m-MIBI SPECT was performed before operation and cardiac events (hard event: cardiac death and myocardial infarction; soft event: ischemic ECG change, congestive heat failure and unstable angina) were surveyed through perioperative periods (14.6{+-}5.6 days). Clinical risk indices, functional capacity, surgery procedures and SPECT findings were tested for their predictive values of perioperative cardiac events. Peri-operative cardiac events occurred in 25 patients (3 hard events and 22 soft events). Clinical risk indices, surgical procedure risks and SPECT findings but functional capacity were predictive of cardiac events. Reversible perfusion decrease was a better predictor than persistent decrease. Multivariate analysis sorted out surgical procedure risk (p=0.0018) and SPECT findings (p=0.0001) as significant risk factors. SPECT could re-stratify perioperative cardiac risks in patients ranked with surgical procedures. We conclude that myocardial SPECT provides additional predictive value to surgical type risks as well as clinical indexes or functional capacity for the prediction of preoperative cardiac events in noncardiac surgery.

  11. [sup 99m]Tc-ECD SPECT study in dementia and aphasia

    Energy Technology Data Exchange (ETDEWEB)

    Kuwabara, Yasuo; Ichiya, Yuichi; Otsuka, Makoto; Sasaki, Masayuki; Akashi, Yuko; Fukumura, Toshimitsu; Yoshida, Tsuyoshi; Masuda, Kouji; Ichimiya, Atsushi (Kyushu Univ., Fukuoka (Japan). Faculty of Medicine)

    1993-09-01

    We studied clinical significance of [sup 99m]Tc-L,L,-ethyl cysteine dimer ([sup 99m]Tc-ECD) SPECT study in dementia and aphasia, and compared it with [sup 99m]Tc-HMPAO SPECT study. The subjects consisted of 13 patients, including 10 patients with dementia and 3 patients with aphasia. Hypoperfusion areas were detected in 5 out of 10 patients with dementia and 2 out of 3 patients with aphasia in [sup 99m]Tc-ECD SPECT, and in 4 out of 10 patients with dementia and all of 3 patients with aphasia in [sup 99m]Tc-HMPAO SPECT. The count rate ratios in [sup 99m]Tc-ECD and [sup 99m]Tc-HMPAO SPECT were correlated well with each other, and the contrast of the [sup 99m]Tc-ECD SPECT image was equivalent or slightly higher as compared with [sup 99m]Tc-HMPAO. Therefore, [sup 99m]Tc-ECD SPECT study was considered to be useful for the evaluation of cerebral perfusion in dementia and aphasia. (author).

  12. The Performance of Ictal Brain SPECT for Localizing Epileptogenic Foci in Temporal Lobe epilepsies

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Eun Sil; Lee, Dong Soo; Chung, June Key; Lee, Myung Chul; Koh, Chang Soon; Chang, Kee Hyun; Lee, Sang Kun; Chung, Chun Kee [Seoul National University College of Medicine, Seoul (Korea, Republic of)

    1995-06-15

    Anterior temporal lobectomy has become a widely used respective surgery in patients with medically intractable temporal lobe epilepsies. Prerequisites of this resection include the accurate localization of the epileptogenic focus and the determination that the proposed resection would not result in unacceptable postoperative memory or language deficits. The purpose of this study was to evaluate the performance of ictal SPECT compared to MRI findings for localization of epileptogenic foci in this group of patients. 11 patients who had been anterior temporal oral lobectomy were evaluated with ictal {sup 99m}Tc-HMPAO SPECT and MRI. MRI showed 8/11(73%) concordant lesion to the side of surgery and ictal SPECT also showed 8/11(73%) concordant hyperperfusion. In 3 cases with incorrect or nonlocalizing findings of MRI, ictal SPECT showed concordant hyperperfusion. In 2 cases confirmed by pre-resectional invasive EEG, MRI showed bilateral and contralateral lesion but ictal SPECT showed concordant hyperperfusion. 3 delayed injection of ictal SPECT showed discordant hyperperfusion. Thus, ictal SPECT was a useful method for localizing epileptogenic foci in temporal lobe epilepsies and appeared complementay to MRI.

  13. A single CdZnTe detector for simultaneous CT/SPECT imaging

    Energy Technology Data Exchange (ETDEWEB)

    Barber, W.C. E-mail: bill@barber.uscf.edu; Iwata, Koji; Hasegawa, B.H.; Bennett, P.R.; Cirignano, L.J.; Shah, K.S

    2003-06-01

    Clinical CT/SPECT systems acquire CT and SPECT data sequentially using different detectors in close proximity to minimise patient movement and interscan delay. We have developed a prototype simultaneous CT/SPECT imager, using a single CdZnTe detector, with the goal of improving image coregistration and decreasing scan time. A 16-pixel CdZnTe detector was operated in pulse-counting mode with 50 ns shaping time. Energy discrimination is used to separate the CT and SPECT data. Simultaneous SPECT and CT images were obtained for a phantom with the X-ray flux limited to reduce pulse pile-up in the radionuclide energy window. At 140 keV, the efficiency and energy resolution are 70% and 10%, respectively, and were constant for fluence rates up to 10{sup 3} cps per detector element for 140 keV gamma rays, but degrade rapidly at higher fluence rates. In pulse-counting mode, the maximum count rate of 10{sup 3} cps per element from the CdZnTe detector is sufficient for SPECT imaging, but is considerably lower than the fluence rates encountered in CT. The smallest lesion visually detectable in SPECT is 9 mm and the CT spatial resolution is smaller than 4.5 mm. Image registration is intrinsic because the data can be acquired simultaneously with a single detector with the same reconstruction geometry.

  14. Tc-{sup 99m} ECD brain SPECT in MELAS syndrome: comparison with MR finding

    Energy Technology Data Exchange (ETDEWEB)

    Park, Sang Joon; Ryu, Young Hoon; Yoon, Pyeong Ho; Jeon, Tae Joo; Kim, Jai Keun; Nam, Ji Eun; Lee, Jong Doo [College of Medicine, Yonsei Univ., Seoul (Korea, Republic of); Lee, Byung Hee [Poondang Cha Hospital, Sungnam (Korea, Republic of); Shin, Hyung Cheol [College of Medicine, Soonchunhyang Univ., Chonnon (Korea, Republic of)

    1998-07-01

    The purpose of this study was to evaluate SPECT findings of MELAS syndrome and mitochondrial myopathy and correlate them with MR findings in search of specific imaging features and to assess the role of SPECT in MELAS syndrome. Five patients (four females and one male; age range, 1 to 25 years) who presented with repeated stroke-like episodes or seizures or developmental delay or were asymptomatic but had elevated lactic acid in CSF and serum were evaluated with conventional noncontrast MR imaging and SPECT. MRI demonstrated increased T2 signal intensities in the affected areas of gray and white matters mainly on the parietal (4/5) and occipital lobes (4/5) and in the basal ganglias (1/5), which were not restricted to a specific vascular territory. SPECT demonstrated decreased uptake of Tc-99m ECD on parietal (5/5) and occipital (4/5) and temporal (2/5) and frontal (1/5) lobe and basal ganglia (2/5) and thalami (2/5). In a patient with mitochondrial myopathy who had normal MRI, decreased perfusion is noted on left parietal area and bilateral thalami. Comparison of the numbers of abnormal findings revealed that decreased perfusion seen on SPECT were more numerous than anatomical abnormalities seen on MRI. SPECT may be a sensitive method for pathophysiological study of metabolic disturbances in MELAS. Moreover, in patients with mitochondrial myopathy without clinical encephalopathy, SPECT may play a role in evaluating subclinical encephalopathy even with normal conventional MR findings.

  15. Detection of Metalloenzyme IMP-4 in Carbapenem-resistant Enterobacter Aerogenes%耐碳青霉烯类抗菌药物产气肠杆菌中发现金属酶IMP-4型

    Institute of Scientific and Technical Information of China (English)

    时东彦; 杨靖; 赵建宏

    2013-01-01

    目的 对1株临床分离的的高水平耐碳青霉烯类抗菌药物产气肠杆菌进行金属酶检测,了解其耐药机制.方法 采用纸片扩散法和E-test法检测耐药性,改良Hodge实验和金属酶实验检测产酶的类型,聚合酶链反应(PCR)检测耐药基因并进行测序,采用转移接合实验对基因阳性的菌株进行转移接合.结果 产气肠杆菌的改良Hodge实验阳性,金属酶(IMP)实验阳性,IMP和CTX-M、IntⅠ基因扩增阳性,经测序证为IMP-4和CTX-M-15,膜孔蛋白基因阴性.结论 耐碳青霉烯类抗菌药物产气肠杆菌的耐药机制是产IMP-4和CTX-M-15,同时存在外膜蛋白的缺失.

  16. Radiotracers for PET and SPECT studies of neurotransmitter systems

    Energy Technology Data Exchange (ETDEWEB)

    Fowler, J.S.

    1991-01-01

    The study of neurotransmitter systems is one of the major thrusts in emission tomography today. The current generation of Positron Emission Tomography (PET) and Single Photon Emission Computed Tomography (SPECT) radiotracers examines neurotransmitter properties from a number of different perspectives including their pre and post synaptic sites and the activity of the enzymes which regulate their concentration. Although the dopamine system has been the most extensively investigated, other neurotransmitter systems including the acetylcholine muscarine, serotonin, benzodiazepine, opiate, NMDA and others are also under intensive development. Enzymes involved in the synthesis and regulation of neurotransmitter concentration, for example monoamine oxidase and amino acid decarboxylase has also been probed in vivo. Medical applications range from the study of normal function and the characterization of neurotransmitter activity in neurological and psychiatric diseases and in heart disease and cancer to the study of the binding of therapeutic drugs and substances of abuse. This chapter will provide an overview of the current generation of radiotracers for PET and SPECT studies of neurotransmitter systems including radiotracer design, synthesis localization mechanisms and applications in emission tomography. 60 refs., 1 tab.

  17. Voxel significance mapping in epilepsy studies using subtraction ictal SPECT

    Science.gov (United States)

    Brinkmann, Benjamin H.; O'Brien, Terence J.; Webster, Desmond B.; Robins, Peter D.; Mullan, Brian P.; Robb, Richard A.

    1999-05-01

    Subtraction ictal SPECT coregistered to MRI (SISCOM) has been shown to aid epileptogenic localization and improve surgical outcomes in partial epilepsy patients. This paper reports a new method of identifying significant areas of epileptogenic activation in the SISCOM subtraction image taking into account normal variation between sequential Tc-99m Ethyl Cysteinate Diethylester SPECT scans of single individuals. The method uses the AIR 3.0 nonlinear registration software to combine a group of subtraction images into a common anatomical framework. A map of the pixel intensity standard deviation values in the subtraction images is created, and this map is nonlinearly registered to a patient's SISCOM subtraction image. Pixels in the patient subtraction image may then be evaluated based upon the statistical characteristics of corresponding pixels in the atlas. Validation experiments were performed to verify that local image variances are not constant across the image and that nonlinear registration preserves local image variances. SISCOM images created with the voxel variance method were rated higher in quality than the conventional image variance method in images from fifteen patients. No difference in localization rate was observed between the voxel variance mapping and image variance methods. The voxel significance mapping method was shown to improve the quality of clinical SISCOM images without removing localizing information.

  18. Applications of SPECT imaging of dopaminergic neurotransmission in neuropsychiatric disorders

    Energy Technology Data Exchange (ETDEWEB)

    Kugaya, Akira; Fujita, Masahiro; Innis, R.B. [Yale Univ., New Haven, CT (United States). School of Medicine

    2000-02-01

    Single photon emission computed tomography (SPECT) tracers selective for pre- and post-synaptic targets have allowed measurements of several aspects of dopaminergic (DA) neurotransmission. In this article, we will first review our DA transporter imaging in Parkinson's disease. We have developed the in vivo dopamine transporter (DAT) imaging with [{sup 123}I]{beta}-CIT ((1R)-2{beta}-Carbomethoxy-3{beta}-(4-iodophenyl)tropane). This method showed that patients with Parkinson's disease have markedly reduced DAT levels in striatum, which correlated with disease severity and disease progression. Second, we applied DA imaging techniques in patients with schizophrenia. Using amphetamine as a releaser of DA, we observed the enhanced DA release, which was measured by imaging D2 receptors with [{sup 123}I]IBZM (iodobenzamide), in schizophrenics. Further we developed the measurement of basal synaptic DA levels by AMPT (alpha-methyl-paratyrosine)-induced unmasking of D2 receptors. Finally, we expanded our techniques to the measurement of extrastriatal DA receptors using [{sup 123}I]epidepride. The findings suggest that SPECT is a useful technique to measure DA transmission in human brain and may further our understanding of the pathophysiology of neuropsychiatric disorders. (author)

  19. First measurements with the neutron decay spectrometer aSPECT

    Energy Technology Data Exchange (ETDEWEB)

    Baessler, S.; Ayala Guardia, F.; Borg, M.; Heil, W.; Konrad, G.; Munoz Horta, R.; Sobolev, Yu. [Universitaet Mainz, Institut fuer Physik, Mainz (Germany); Glueck, F. [IEKP, Universitaet Karlsruhe, Karlsruhe (Germany); Theory Department, Research Institute for Nuclear and Particle Physics, Budapest 114 (Hungary); Konorov, I.; Petzoldt, G.; Simson, M.; Wirth, H.F.; Zimmer, O. [Physik-Department, Technische Universitaet Muenchen, Garching (Germany); Rich, D. [Forschungsneutronenquelle Heinz Maier-Leibnitz, Garching (Germany)

    2008-10-15

    The neutron decay spectrometer aSPECT has been built to perform a precise measurement of the proton spectrum shape in the decay of free neutrons. Such a measurement allows a determination of the neutrino electron angular-correlation coefficient a. The present best experiments have an uncertainty of {delta}a/a=5% and since the seventies there is no substantial improvement. With aSPECT, we aim for an uncertainty which is lower by more than an order of magnitude, thus enabling us to perform several precise tests of the Standard Model. In our first beam time at the particle physics beam MEPHISTO at the Forschungsneutronenquelle Heinz Maier-Leibnitz, we studied the properties of the spectrometer. The most serious problem turned out to be the situation- and time-dependent behavior of the background. From the data sets from this beam time in which a background problem was not obvious, we could extract a value of a=-0.1151{+-}0.0040{sup stat}, but we could not quantify the background uncertainty. We show ways to deal with the background and other problems for future beam times. (orig.)

  20. First measurements with the neutron decay spectrometer a SPECT

    Science.gov (United States)

    Baeßler, S.; Ayala Guardia, F.; Borg, M.; Glück, F.; Heil, W.; Konrad, G.; Konorov, I.; Muñoz Horta, R.; Petzoldt, G.; Rich, D.; Simson, M.; Sobolev, Yu.; Wirth, H.-F.; Zimmer, O.

    2008-10-01

    The neutron decay spectrometer a SPECT has been built to perform a precise measurement of the proton spectrum shape in the decay of free neutrons. Such a measurement allows a determination of the neutrino electron angular-correlation coefficient a . The present best experiments have an uncertainty of Δ a/ a = 5% and since the seventies there is no substantial improvement. With a SPECT, we aim for an uncertainty which is lower by more than an order of magnitude, thus enabling us to perform several precise tests of the Standard Model. In our first beam time at the particle physics beam MEPHISTO at the Forschungsneutronenquelle Heinz Maier-Leibnitz, we studied the properties of the spectrometer. The most serious problem turned out to be the situation- and time-dependent behavior of the background. From the data sets from this beam time in which a background problem was not obvious, we could extract a value of a = - 0.1151±0.0040stat , but we could not quantify the background uncertainty. We show ways to deal with the background and other problems for future beam times.

  1. Myocardial Perfusion SPECT Imaging in Patients after Percutaneous Coronary Intervention.

    Science.gov (United States)

    Georgoulias, Panagiotis; Valotassiou, Varvara; Tsougos, Ioannis; Demakopoulos, Nikolaos

    2010-05-01

    Coronary artery disease (CAD) is the most prevalent form of cardiovascular disease affecting about 13 million Americans, while more than one million percutaneous transluminal intervention (PCI) procedures are performed annually in the USA. The relative high occurrence of restenosis, despite stent implementation, seems to be the primary limitation of PCI. Over the last decades, single photon emission computed tomography (SPECT) myocardial perfusion imaging (MPI), has proven an invaluable tool for the diagnosis of CAD and patients' risk stratification, providing useful information regarding the decision about revascularization and is well suited to assess patients after intervention. Information gained from post-intervention MPI is crucial to differentiate patients with angina from those with exo-cardiac chest pain syndromes, to assess peri-intervention myocardial damage, to predict-detect restenosis after PCI, to detect CAD progression in non-revascularized vessels, to evaluate the effects of intervention if required for occupational reasons and to evaluate patients' long-term prognosis. On the other hand, chest pain and exercise electrocardiography are largely unhelpful in identifying patients at risk after PCI.Although there are enough published data demonstrating the value of myocardial perfusion SPECT imaging in patients after PCI, there is still debate on whether or not these tests should be performed routinely.

  2. Sequential MRI, SPECT and PET in respiratory syncytial virus encephalitis

    Energy Technology Data Exchange (ETDEWEB)

    Hirayama, K.; Sakazaki, Hiromi; Murakami, Seiko; Yonezawa, Sumiko [Department of Paediatrics, Izumi Municipal Hospital, Osaka (Japan); Fujimoto, Keiji [Dept. of Radiology, Izumi Municipal Hospital, Osaka (Japan); Seto, Toshiyuki; Tanaka, Katsuji; Hattori, Hideji; Matsuoka, Osamu [Dept. of Paediatrics, Osaka City University Medical School, Osaka (Japan); Murata, Ryosuke [Children`s Medical Centre, Osaka City General Hospital, Osaka (Japan)

    1999-04-01

    We report on a 3-year-old girl with respiratory syncytial virus (RSV) encephalitis manifested by disturbance of consciousness, conjugate eye deviation, anuria, truncal ataxia and intention tremor. T2-weighted magnetic resonance imaging (MRI) showed hyperintense areas in the cerebellar cortex. No lesion was detected in the cerebral cortex, pons or spinal cord. The hyperintense areas in the cerebellar cortex diminished with recovery from the clinical manifestations and had resolved 2 months after onset. The MRI lesions in the cerebellum were considered to be due to oedema. SPECT and positron emission tomography (PET), performed 3 months after onset, disclosed areas of hypoperfusion and hypometabolism at the same sites. One year after onset, MRI showed mild atrophy of the cerebellum. Hypoperfusion on SPECT and hypometabolism on PET remained. Neuroimaging showed that ataxia and tremor in this case were the result of cerebellitis. The patient has no neurological deficit except for mild truncal ataxia. This patient is a rare example of RSV encephalitis. (orig.) With 4 figs., 16 refs.

  3. aSPECT - Measuring the proton spectrum in neutron decay

    Energy Technology Data Exchange (ETDEWEB)

    Simson, Martin; Leung, Kent; Zimmer, Oliver [Institut Laue-Langevin, Grenoble (France); Physik-Department E18, TU, Muenchen (Germany); Ayala Guardia, Fidel; Borg, Michael; Heil, Werner; Konrad, Gertrud; Munoz Horta, Raquel; Sobolev, Yury [Institut fuer Physik, Universitaet Mainz (Germany); Baessler, Stefan [University of Virginia, Charlottesville, VA (United States); Glueck, Ferenc [IEKP, Universitaet Karlsruhe (Germany); Konorov, Igor [Physik-Department E18, TU, Muenchen (Germany); Soldner, Torsten [Institut Laue-Langevin, Grenoble (France); Wirth, Hans-Friedrich [Fakultaet fuer Physik, LMU, Muenchen (Germany)

    2009-07-01

    With the aSPECT spectrometer we measure the proton recoil spectrum in the decay of the free neutron. Its shape depends on the angular correlation between the momenta of the antineutrino and electron for kinematic reasons. A precision measurement of this correlation coefficient a allows to test the unitarity of the CKM matrix and provides limits on the existence of scalar and tensor currents. aSPECT is a retardation spectrometer, i.e. the proton recoil spectrum is measured by counting all decay protons that overcome a potential barrier. By varying the height of the barrier the shape of the proton spectrum can be reconstructed. After the barrier the protons are accelerated to {proportional_to}15 keV and detected by a silicon drift detector. Unpolarized cold neutrons are guided through the decay volume of the spectrometer where a fraction of them decays. The recoil protons produced in these decays are guided to the detector by magnetic field lines. In the last beam time a statistical accuracy of about 2 % per 24 hours measurement time was reached, the total error is expected to be well below 5 %. Details of the spectrometer setup as well as the status of the ongoing data analysis are presented in the talk.

  4. Simultaneous reconstruction and segmentation for dynamic SPECT imaging

    Science.gov (United States)

    Burger, Martin; Rossmanith, Carolin; Zhang, Xiaoqun

    2016-10-01

    This work deals with the reconstruction of dynamic images that incorporate characteristic dynamics in certain subregions, as arising for the kinetics of many tracers in emission tomography (SPECT, PET). We make use of a basis function approach for the unknown tracer concentration by assuming that the region of interest can be divided into subregions with spatially constant concentration curves. Applying a regularised variational framework reminiscent of the Chan-Vese model for image segmentation we simultaneously reconstruct both the labelling functions of the subregions as well as the subconcentrations within each region. Our particular focus is on applications in SPECT with the Poisson noise model, resulting in a Kullback-Leibler data fidelity in the variational approach. We present a detailed analysis of the proposed variational model and prove existence of minimisers as well as error estimates. The latter apply to a more general class of problems and generalise existing results in literature since we deal with a nonlinear forward operator and a nonquadratic data fidelity. A computational algorithm based on alternating minimisation and splitting techniques is developed for the solution of the problem and tested on appropriately designed synthetic data sets. For those we compare the results to those of standard EM reconstructions and investigate the effects of Poisson noise in the data.

  5. New SPECT and PET Radiopharmaceuticals for Imaging Cardiovascular Disease

    Directory of Open Access Journals (Sweden)

    Oyebola O. Sogbein

    2014-01-01

    Full Text Available Nuclear cardiology has experienced exponential growth within the past four decades with converging capacity to diagnose and influence management of a variety of cardiovascular diseases. Single photon emission computed tomography (SPECT myocardial perfusion imaging (MPI with technetium-99m radiotracers or thallium-201 has dominated the field; however new hardware and software designs that optimize image quality with reduced radiation exposure are fuelling a resurgence of interest at the preclinical and clinical levels to expand beyond MPI. Other imaging modalities including positron emission tomography (PET and magnetic resonance imaging (MRI continue to emerge as powerful players with an expanded capacity to diagnose a variety of cardiac conditions. At the forefront of this resurgence is the development of novel target vectors based on an enhanced understanding of the underlying pathophysiological process in the subcellular domain. Molecular imaging with novel radiopharmaceuticals engineered to target a specific subcellular process has the capacity to improve diagnostic accuracy and deliver enhanced prognostic information to alter management. This paper, while not comprehensive, will review the recent advancements in radiotracer development for SPECT and PET MPI, autonomic dysfunction, apoptosis, atherosclerotic plaques, metabolism, and viability. The relevant radiochemistry and preclinical and clinical development in addition to molecular imaging with emerging modalities such as cardiac MRI and PET-MR will be discussed.

  6. SPECT reconstruction using DCT-induced tight framelet regularization

    Science.gov (United States)

    Zhang, Jiahan; Li, Si; Xu, Yuesheng; Schmidtlein, C. R.; Lipson, Edward D.; Feiglin, David H.; Krol, Andrzej

    2015-03-01

    Wavelet transforms have been successfully applied in many fields of image processing. Yet, to our knowledge, they have never been directly incorporated to the objective function in Emission Computed Tomography (ECT) image reconstruction. Our aim has been to investigate if the ℓ1-norm of non-decimated discrete cosine transform (DCT) coefficients of the estimated radiotracer distribution could be effectively used as the regularization term for the penalized-likelihood (PL) reconstruction, where a regularizer is used to enforce the image smoothness in the reconstruction. In this study, the ℓ1-norm of 2D DCT wavelet decomposition was used as a regularization term. The Preconditioned Alternating Projection Algorithm (PAPA), which we proposed in earlier work to solve penalized likelihood (PL) reconstruction with non-differentiable regularizers, was used to solve this optimization problem. The DCT wavelet decompositions were performed on the transaxial reconstructed images. We reconstructed Monte Carlo simulated SPECT data obtained for a numerical phantom with Gaussian blobs as hot lesions and with a warm random lumpy background. Reconstructed images using the proposed method exhibited better noise suppression and improved lesion conspicuity, compared with images reconstructed using expectation maximization (EM) algorithm with Gaussian post filter (GPF). Also, the mean square error (MSE) was smaller, compared with EM-GPF. A critical and challenging aspect of this method was selection of optimal parameters. In summary, our numerical experiments demonstrated that the ℓ1-norm of discrete cosine transform (DCT) wavelet frame transform DCT regularizer shows promise for SPECT image reconstruction using PAPA method.

  7. Detection of Sentinel Lymph Nodes in Gynecologic Tumours by Planar Scintigraphy and SPECT/CT

    Directory of Open Access Journals (Sweden)

    Otakar Kraft

    2012-08-01

    Full Text Available Objective: Assess the role of planar lymphoscintigraphy and fusion imaging of SPECT/CT in sentinel lymph node (SLN detection in patients with gynecologic tumours. Material and Methods: Planar scintigraphy and hybrid modality SPECT/CT were performed in 64 consecutive women with gynecologic tumours (mean age 53.6 with range 30-77 years: 36 pts with cervical cancer (Group A, 21 pts with endometrial cancer (Group B, 7 pts with vulvar carcinoma (Group C. Planar and SPECT/CT images were interpreted separately by two nuclear medicine physicians. Efficacy of these two techniques to image SLN were compared. Results: Planar scintigraphy did not image SLN in 7 patients (10.9%, SPECT/CT was negative in 4 patients (6.3%. In 35 (54.7% patients the number of SLNs captured on SPECT/CT was higher than on planar imaging. Differences in detection of SLN between planar and SPECT/CT imaging in the group of all 64 patients are statistically significant (p<0.05. Three foci of uptake (1.7% from totally visible 177 foci on planar images in 2 patients interpreted on planar images as hot LNs were found to be false positive non-nodal sites of uptake when further assessed on SPECT/CT. SPECT/CT showed the exact anatomical location of all visualised sentinel nodes. Conclusion: In some patients with gynecologic cancers SPECT/CT improves detection of sentinel lymph nodes. It can image nodes not visible on planar scintigrams, exclude false positive uptake and exactly localise pelvic and paraaortal SLNs. It improves anatomic localization of SLNs. (MIRT 2012;21:47-55

  8. V/P SPECT as a diagnostic tool for pregnant women with suspected pulmonary embolism

    Energy Technology Data Exchange (ETDEWEB)

    Bajc, Marika; Olsson, Berit; Joegi, Jonas [Skaane University Hospital and Lund University, Clinical Physiology and Nuclear Medicine, Lund (Sweden); Gottsaeter, Anders [Skaane University Hospital, Vascular Diseases, Malmoe (Sweden); Hindorf, Cecilia [Skaane University Hospital, Radiation Physics, Lund (Sweden)

    2015-07-15

    The purpose of the study was to assess the prevalence of pulmonary embolism (PE) and other lung diseases among pregnant women with suspected PE and to calculate the radiation exposure to patient and fetus in this population. As a secondary aim, we evaluated the negative predictive value of a normal ventilation/perfusion single photon emission computed tomography (V/P SPECT) examination in pregnancy. We studied all 127 pregnant women who had suspected PE and had undergone V/P SPECT at our institution in the course of a 5-year period. Radiation exposure to patient and fetus and the negative predictive value of a normal V/P SPECT examination were also measured. V/P SPECT identified PE in 11 women (9 %). Moreover, in 15 women (12 %) the examination revealed pneumonia (in 2 cases in addition to PE) and in 1 woman signs of airway obstruction were revealed. Among the 116/127 women (91 %) where PE was ruled out by V/P SPECT, none was diagnosed subsequently with PE or deep venous thrombosis (DVT) during the same pregnancy or puerperal period. For P SPECT, the calculated fetal absorbed dose was < 0.6 mGy,and the calculated breast absorbed dose 0.6 mGy. For V SPECT, the calculated fetal absorbed dose was < 0.014 mGy and the breast absorbed dose 0.25 mGy. The prevalence of PE was low (9 %) among pregnant women with suspected disease. Pneumonia was diagnosed in 12 % of patients. The negative predictive value of V/P SPECT was high, and the radiation exposure from V/P SPECT was low both for fetus and patient. (orig.)

  9. Onboard functional and molecular imaging: A design investigation for robotic multipinhole SPECT

    Energy Technology Data Exchange (ETDEWEB)

    Bowsher, James, E-mail: james.bowsher@duke.edu; Giles, William; Yin, Fang-Fang [Department of Radiation Oncology, Duke University Medical Center, Durham, North Carolina 27710 and Medical Physics Graduate Program, Duke University, Durham, North Carolina 27710 (United States); Yan, Susu [Medical Physics Graduate Program, Duke University, Durham, North Carolina 27710 (United States); Roper, Justin [Department of Radiation Oncology, Duke University Medical Center, Durham, North Carolina 27710 (United States)

    2014-01-15

    Purpose: Onboard imaging—currently performed primarily by x-ray transmission modalities—is essential in modern radiation therapy. As radiation therapy moves toward personalized medicine, molecular imaging, which views individual gene expression, may also be important onboard. Nuclear medicine methods, such as single photon emission computed tomography (SPECT), are premier modalities for molecular imaging. The purpose of this study is to investigate a robotic multipinhole approach to onboard SPECT. Methods: Computer-aided design (CAD) studies were performed to assess the feasibility of maneuvering a robotic SPECT system about a patient in position for radiation therapy. In order to obtain fast, high-quality SPECT images, a 49-pinhole SPECT camera was designed which provides high sensitivity to photons emitted from an imaging region of interest. This multipinhole system was investigated by computer-simulation studies. Seventeen hot spots 10 and 7 mm in diameter were placed in the breast region of a supine female phantom. Hot spot activity concentration was six times that of background. For the 49-pinhole camera and a reference, more conventional, broad field-of-view (FOV) SPECT system, projection data were computer simulated for 4-min scans and SPECT images were reconstructed. Hot-spot localization was evaluated using a nonprewhitening forced-choice numerical observer. Results: The CAD simulation studies found that robots could maneuver SPECT cameras about patients in position for radiation therapy. In the imaging studies, most hot spots were apparent in the 49-pinhole images. Average localization errors for 10-mm- and 7-mm-diameter hot spots were 0.4 and 1.7 mm, respectively, for the 49-pinhole system, and 3.1 and 5.7 mm, respectively, for the reference broad-FOV system. Conclusions: A robot could maneuver a multipinhole SPECT system about a patient in position for radiation therapy. The system could provide onboard functional and molecular imaging with 4-min

  10. IMP-8 observations of traveling compressions regions: New evidence for near-earth plasmoids and neutral lines

    Energy Technology Data Exchange (ETDEWEB)

    Slavin, J.A.; Lepping, R.P.; Baker, D.N. (NASA/Goddard Space Flight Center, Greenbelt, MD (USA))

    1990-06-01

    An examination of IMP-8 tail lobe magnetic field measurements has been conducted to determine whether the traveling compressions region (TCR) phenomena detected by ISEE-3 in the distant geotail, and believed to be caused by tailward moving plasmoids, are present closer to the earth. The study produced 16 examples of TCRs at distances of X = {minus}31 to {minus}37 R{sub E}. For two events considered in detail TCRs were observed in close association with substorm growth phase signatures in the lobes. The lengths of these TCRs are estimated to be 8-12 R{sub E}. It is their conclusion that the IMP-8 TCR observations provide new evidence that small plasmoids and, hence, multiple reconnection neutral lines can sometimes exist earthward of X = {minus}35 R{sub E}.

  11. Detection of SPM and IMP metallo-β-lactamases in clinical specimens of Pseudomonas aeruginosa from a Brazilian public tertiary hospital

    Directory of Open Access Journals (Sweden)

    Carlos Henrique Camargo

    2011-10-01

    Full Text Available Phenotypic and genotypic SPM and IMP metallo-β-lactamases (MBL detection and also the determination of minimal inhibitory concentrations (MIC to imipenem, meropenem and ceftazidime were evaluated in 47 multidrug-resistant Pseudomonas aeruginosa isolates from clinical specimens. Polymerase chain reaction detected 14 positive samples to either blaSPM or blaIMP genes, while the best phenotypic assay (ceftazidime substrate and mercaptopropionic acid inhibitor detected 13 of these samples. Imipenem, meropenem and ceftazidime MICs were higher for MBL positive compared to MBL negative isolates. We describe here the SPM and IMP MBL findings in clinical specimens of P. aeruginosa from the University Hospital of Botucatu Medical School, São Paulo, Brazil, that reinforce local studies showing the high spreading of blaSPM and blaIMP genes among brazilian clinical isolates.

  12. Tc-99m-bicisate (ECD)-brain-SPECT in rapidly progressive dementia; Hirn-SPECT mit Tc-99m-Bicisat (ECD) bei rasch progredientem dementiellen Syndrom

    Energy Technology Data Exchange (ETDEWEB)

    Marienhagen, J.; Eilles, C. [Regensburg Univ. (Germany). Abt. fuer Nuklearmedizin; Weingaertner, U.; Blaha, L. [Bezirkskrankenhaus Mainkofen (Germany). Psychiatrische Klinik; Zerr, I.; Poser, S. [Goettingen Univ. (Germany). Klinik und Poliklinik fuer Neurologie

    1999-07-01

    We present a 61-year-old male patient with progressive dementia. A brain SPECT with Tc-99m-bicisate was performed for confirmation of clinically suspected Alzheimer-dementia. At the time of the SPECT-investigation marked apraxia and aphasia besides severe dementia were present. Electrophysiological as well as anatomical neuroimaging findings showed non-diagnostic alterations. SPECT revealed distinct perfusion defects, which made Alzheimer Dementia unlikely. The further course of the patient was determined by rapidly progressive deterioration with development of akinetic mutism. Thereafter, increased levels of neuron-specific enolase as well as 14-3-3 proteins were found in the cerebro-spinal fluid (CSF). The patient finally died with signs of cerebral decortication. Due to the clinical course and the CSF-findings the patient's final diagnosis was Creutzfeld-Jakob-disease, nevertheless no autopsy was performed. The presented case report underscores the clinical utility of perfusion brain SPECT in the differential diagnosis of dementias. (orig.) [German] Wir berichten ueber einen 61jaehrigen Patienten mit progredientem dementiellen Syndrom, der unter der Verdachtsdiagnose einer Demenz vom Alzheimer-Typ (DAT) zur Hirn-SPECT-Untersuchung mit TC-99m-Bicisat (ECD) vorgestellt wurde. Zum Untersuchungszeitpunkt bestanden neben dem Vollbild einer Demenz eine ausgepraegte Apraxie und Aphasie bei unspezifischen Veraenderungen im EEG sowie der neuroradiologischen Bildgebung. In der Hirn-SPECT-Untersuchung fanden sich fuer eine DAT untypische ausgedehnte, vorwiegend rechtshemisphaerische Perfusionsstoerungen. Im weiteren Verlauf rasche Progredienz des Krankheitsbildes mit Entwicklung eines akinetischen Mutismus sowie Nachweis erhoehter Werte der neuronspezifischen Enolase und des 14-3-3-Proteins im Liquor. Der Patient verstarb schliesslich unter dem Bild einer Decortication. Aufgrund des klinischen Verlaufs sowie der Liquorbefunde wurde, da eine autoptische Befundsicherung

  13. Emerging Carbapenem-Resistant Pseudomonas aeruginosa Isolates Carrying blaIMP Among Burn Patients in Isfahan, Iran

    Science.gov (United States)

    Radan, Mohsen; Moniri, Rezvan; Khorshidi, Ahmad; Gilasi, Hamidreza; Norouzi, Zohreh; Beigi, Fahimeh; Dasteh Goli, Yasaman

    2016-01-01

    Background Metallo-β-lactamase (MBL)-producing Pseudomonas aeruginosa is a significant pathogen in burn patients. Objectives The aim of this study was to determine the prevalence of carbapenem-resistant P. aeruginosa isolates, including those resistant to imipenemase (IMP), in a burn unit in Isfahan, Iran. Patients and Methods One hundred and fifty P. aeruginosa isolates from burn patients were tested for antibiotic susceptibility by the disc diffusion method in accordance with CLSI guidelines. Production of MBL was identified with the EDTA disk method. DNA was purified from the MBL-positive isolates, and detection of the blaIMP gene was performed with PCR. Results Fifty-seven out of 150 (38%) isolates were multi-drug resistant (MDR), and 93 (62%) were extensively-drug resistant (XDR). Among all isolates, the resistance rate to ciprofloxacin, tobramycin, imipenem, meropenem, amikacin, ceftazidime, and cefepime was higher than 90%, while the resistance rates to piperacillin/tazobactam and aztreonam were 70.7% and 86%, respectively. Colistin and polymyxin B remained the most effective studied antibiotics. All of the imipenem-resistant P. aeruginosa isolates were MBL-positive, and 107 out of 144 (74.3%) of the MBL isolates were positive for the blaIMP gene. Conclusions The results of this study show that the rate of P. aeruginosa-caused burn wound infections was very high, and many of the isolates were resistant to three or more classes of antimicrobials. Such extensive resistance to antimicrobial classes is important because few treatment options remain for patients with burn wound infections. blaIMP-producing P. aeruginosa isolates are a rising threat in burn-care units, and should be controlled by conducting infection-control assessments.

  14. Social Networks of Researchers in Business To Business Marketing: A Case Study of the IMP Group 1984-1999

    OpenAIRE

    Piera Morlacchi; Wilkinson, Ian F.; Louise Young

    2004-01-01

    Science is a social process that functions through social networks of researchers that form invisible colleges. Analysis of these social networks provides a means for examining the structure of relations among researchers. The Industrial Marketing and Purchasing (IMP) group, "an informal international group of scholars concerned with developing concepts and knowledge in the field of business-to-business marketing and purchasing," is used as a case study of a network of researchers because it ...

  15. Noninvasive Nuclear SPECT Myocardial Blood Flow Quantitation to Guide Management for Coronary Artery Disease.

    Science.gov (United States)

    Chen, Lung-Ching; Jong, Bor-Hsin; Lin, Sheng-Che; Ku, Chi-Tai; Chen, Ing-Jou; Chen, Yen-Kung; Hsu, Bailing

    2017-09-01

    Recently, myocardial blood flow quantitation with dynamic SPECT has been validated to enhance the detection of multivessel coronary artery disease (CAD) and conclude equivocal SPECT myocardial perfusion study. This advance opened an important clinical application to utilize the tool in guiding CAD management for area where myocardial perfusion tracers for PET are unavailable or unaffordable. We present a clinical patient with ongoing recursive angina who underwent multiple nuclear stress tests for a sequence of CAD evaluation in 26 months and demonstrated that SPECT myocardial blood flow quantitation properly guided CAD management to warrant patient outcome.

  16. Brain PET and technetium-99m-ECD SPECT imaging in Lhermitte-Duclos disease

    Energy Technology Data Exchange (ETDEWEB)

    Ogasawara, K.; Yasuda, S.; Beppu, T.; Kobayashi, M.; Doi, M.; Kuroda, K.; Ogawa, A. [Dept. of Neurosurgery, Iwate Medical Univ., Morioka (Japan)

    2001-11-01

    Two patients with Lhermitte-Duclos disease were evaluated by brain positron emission tomography (PET) and technetium-99m-ethyl cysteinate dimer ({sup 99m}Tc-ECD) single-photon emission computed tomography (SPECT). In the lesions in both patients, hyperperfusion was detected on cerebral blood flow images obtained by PET, and hyperactivity by standard {sup 99m}Tc-ECD SPECT. Dynamic {sup 99m}Tc-ECD SPECT images demonstrated a plateau of activity in each lesion. These findings suggest that lesions in Lhermitte-Duclos disease have a retention mechanism for {sup 99m}Tc-ECD equivalent to that of normal neural tissue. (orig.)

  17. Lung function assessment using Xe-133 dynamic SPECT in dual-camera system

    Energy Technology Data Exchange (ETDEWEB)

    Sakaji, Katsuyuki; Akiyama, Masayuki; Nakazawa, Yasuo [Showa Univ., Tokyo (Japan). Hospital; Umeda, Hirotaka; Takenaka, Haruki; Shinozuka, Akira

    2001-09-01

    The purpose of this study was to estimate lung regional function using Xe-133 dynamic SPECT. SPECT equipment with a dual camera was used. Fourteen rotation acquisitions were obtained beginning immediately after Xe-133 gas inhalation. The time activity curve of each pixel was obtained, and T{sub 1/2} of the washout phase was calculated and mapped. Residual radioactivity was evaluated. Adequate images could be obtained at 30 seconds per rotation even with the dual-camera system. Mapping of T{sub 1/2} allowed temporal changes on one image. Three-dimensional evaluation could be made on a SPECT system using our method. (author)

  18. Contribution of V/Q SPECT to planar scintigraphy in the diagnosis of pulmonary embolism.

    Science.gov (United States)

    Quirce, R; Ibáñez-Bravo, S; Jiménez-Bonilla, J; Martínez-Rodríguez, I; Martínez-Amador, N; Ortega-Nava, F; Lavado-Pérez, C; Bravo-Ferrer, Z; Carril, J M

    2014-01-01

    To evaluate the feasibility of V/Q SPECT and analyze its contribution to planar V/Q lung scintigraphy in the diagnosis of pulmonary embolism (PE). A total of 109 patients with suspected PE showing Wells score>2 and elevated D-dimer were studied. The V/Q could not be completed in 7 patients, so they were excluded. Ventilation and perfusion scans were done using Technegas and (99m)Tc-MAA. Planar study included 8 projections on a 256×256 matrix and 128 projections on a 128×128 matrix were acquired for the SPECT study, applying an iterative method. Planar images were interpreted according to modified PIOPED criteria, and SPECT by the guidelines of the EANMMI. The results with both techniques were compared. V/Q planar scintigraphy and SPECT could be performed in 102 patients. V/Q planar scintigraphy was considered "diagnostic" in 39 of the 102 patients, and "non-diagnostic" in 63. Of the 39 "diagnostic" studies, 31 were reported as high probability of PE and 8 as normal. Of the 63 "non-diagnostic", 26 corresponded to intermediate, 29 to low, and 8 to very low probability. The SPECT study was "diagnostic" in 97 and indeterminate in only 5. All patients with a high probability planar scintigraphy had a positive SPECT. In the 8 patients with a normal planar scintigraphy SPECT was negative in 5 and positive in 3. In the 63 patients with a "non-diagnostic" planar scintigraphy SPECT was "diagnostic" in 58 of them, positive in 17 and negative in 41. V/Q SPECT is a feasible technique as it was performed in 102 of the 109 patients who were enrolled in the study (94%). The addition of V/Q SPECT to planar V/Q decreases the number of "non-diagnostic" reports from 62% in planar scintigraphy to 4.9% in SPECT. Therefore, V/Q SPECT should be included in the diagnosis approach of PE due to its high diagnostic yield. Copyright © 2013 Elsevier España, S.L. and SEMNIM. All rights reserved.

  19. Development of a high-resolution detection module for the INSERT SPECT/MRI system

    Energy Technology Data Exchange (ETDEWEB)

    Busca, Paolo; Fiorini, Carlo; Butt, Arslan D; Occhipinti, Michele; Quaglia, Riccardo; Trigilio, Paolo [Politecnico di Milano, Dipartimento di Elettronica, Informazione e Bioingegneria, Via Golgi 40, 20133 Milano (Italy); Istituto Nazionale di Fisica Nucleare, Sezione di Milano, Via Celoria 16, 20133 Milano (Italy); Nemeth, Gabor; Major, Peter; Bukki, Tamas; Nagy, Kalman [Mediso Medical Imaging Systems, Alsotorokvesz 14, H-1022 Budapest (Hungary); Piemonte, Claudio; Ferri, Alessandro; Gola, Alberto [Fondazione Bruno Kessler (FBK), Via Sommarive, 18, 38123 Trento (Italy); Rieger, Jan [MRI.TOOLS GmbH, Robert-Roessle-Str. 10, 13125 Berlin (Germany); Niendorf, Thoralf [MRI.TOOLS GmbH, Robert-Roessle-Str. 10, 13125 Berlin (Germany); Berlin Ultrahigh Field Facility (B.UniversityF.F.), Max-Delbrueck-Center for Molecular Medicine, Berlin (Germany)

    2014-07-29

    A new multi-modality imaging tool is under development in the framework of the INSERT (Integrated SPECT/MRI for Enhanced Stratification in Radio-chemo Therapy) project, supported by the European Community. The final goal is to develop a custom SPECT apparatus that can be used as an insert for commercially available MRI systems. INSERT is expected to offer more effective and earlier diagnosis with potentially better outcome in survival for the treatment of brain tumors, primarily glioma. Two SPECT prototypes are being developed, one dedicated to preclinical imaging (7 and 9.4 T), the second one dedicated to clinical imaging (3 T).

  20. Performance analysis of a low-cost small animal PET/SPECT scanner

    Energy Technology Data Exchange (ETDEWEB)

    Guerra, Pedro [B104 ETS de Telecomuniacion, Universidad Politecnica de Madrid, 28040 Madrid (Spain)]. E-mail: pguerra@die.upm.es; Rubio, Jose L. [B104 ETS de Telecomuniacion, Universidad Politecnica de Madrid, 28040 Madrid (Spain); Ortuno, Juan E. [B104 ETS de Telecomuniacion, Universidad Politecnica de Madrid, 28040 Madrid (Spain); Kontaxakis, Georgios [B104 ETS de Telecomuniacion, Universidad Politecnica de Madrid, 28040 Madrid (Spain); Ledesma, Maria J. [B104 ETS de Telecomuniacion, Universidad Politecnica de Madrid, 28040 Madrid (Spain); Santos, Andres [B104 ETS de Telecomuniacion, Universidad Politecnica de Madrid, 28040 Madrid (Spain)

    2007-02-01

    In this work the performance of novel small animal positron/single-photon emission (PET/SPECT) scanner is estimated via Monte Carlo simulation, considering a YAP/LSO phoswich detector. To overcome the differences between PET and SPECT and in order to simplify the design, the system implements most signal processing digitally with programmable devices. The estimated performance of the described setup, expressed in terms of spatial image resolution and sensitivity, is 1.4 mm/0.6% for PET and 2.5 mm/0.025% for SPECT, figures that are comparable with state of the art dedicated scanners.

  1. Increased parasellar activity on gallium SPECT is not specific for active cluster headache

    Energy Technology Data Exchange (ETDEWEB)

    Sianard-Gainko, J.; Milet, J.; Ghuysen, V.; Schoenen, J. (Univ. Departments of Neurology and Nuclear Medicine, Liege (Belgium))

    1994-04-01

    The authors have performed gallium SPECT head scans in 30 successive cluster headache (CH) patients and in 7 migraineurs without aura. Parasellar hyperactivity was judged as present in 81% of chronic CH patients. 54% of episodic CH patients in an active period, 56% of episodic CH patients in remission and 71% of migraineurs. No significant correlations were found between the SPECT images and the duration of the disease, of cluster periods or of remissions. Increased parasellar activity on gallium SPECT is thus not specific for CH, nor for the active period of episodic CH. The method lacks reliability for investigation of putative cavernous sinus inflammation. 4 refs., 1 tab.

  2. SPECT/CT imaging in bone scintigraphy of a case of clavicular osteoma

    Directory of Open Access Journals (Sweden)

    Yuka Yamamoto

    2014-05-01

    diphosphonate (HMDP. Whole-body image showed a focus of intensely increased uptake in the clavicle. Single photon emission computed tomography/ computed tomography (SPECT/CT images were also acquired and clearly showed intense uptake at the tumor site. Integrated SPECT/CT imaging supplies both functional and anatomic information about bone: the SPECT imaging improves sensitivity compared with planar imaging, the CT imaging provides precise localization of the abnormal uptake, and information on the shape and structure of the abnormalities improves the specificity of the diagnosis.

  3. The effect of scatter correction on {sup 123}I-IMP brain perfusion SPET with the triple energy window method in normal subjects using SPM analysis

    Energy Technology Data Exchange (ETDEWEB)

    Shiga, Tohru; Takano, Akihiro; Tsukamoto, Eriko; Tamaki, Nagara [Department of Nuclear Medicine, Hokkaido University School of Medicine, Sapporo (Japan); Kubo, Naoki [Department of Radiological Technology, College of Medical Technology, Hokkaido University, Sapporo (Japan); Kobayashi, Junko; Takeda, Yoji; Nakamura, Fumihiro; Koyama, Tsukasa [Department of Psychiatry and Neurology, Hokkaido University School of Medicine, Sapporo (Japan); Katoh, Chietsugu [Department of Tracer Kinetics, Hokkaido University School of Medicine, Sapporo (Japan)

    2002-03-01

    Scatter correction (SC) using the triple energy window method (TEW) has recently been applied for brain perfusion single-photon emission tomography (SPET). The aim of this study was to investigate the effect of scatter correction using TEW on N-isopropyl-p-[{sup 123}I]iodoamphetamine ({sup 123}I-IMP) SPET in normal subjects. The study population consisted of 15 right-handed normal subjects. SPET data were acquired from 20 min to 40 min after the injection of 167 MBq of IMP, using a triple-head gamma camera. Images were reconstructed with and without SC. 3D T1-weighted magnetic resonance (MR) images were also obtained with a 1.5-Tesla scanner. First, IMP images with and without SC were co-registered to the 3D MRI. Second, the two co-registered IMP images were normalised using SPM96. A t statistic image for the contrast condition effect was constructed. We investigated areas using a voxel-level threshold of 0.001, with a corrected threshold of 0.05. Compared with results obtained without SC, the IMP distribution with SC was significantly decreased in the peripheral areas of the cerebellum, the cortex and the ventricle, and also in the lateral occipital cortex and the base of the temporal lobe. On the other hand, the IMP distribution with SC was significantly increased in the anterior and posterior cingulate cortex, the insular cortex and the medial part of the thalamus. It is concluded that differences in the IMP distribution with and without SC exist not only in the peripheral areas of the cerebellum, the cortex and the ventricle but also in the occipital lobe, the base of the temporal lobe, the insular cortex, the medial part of the thalamus, and the anterior and posterior cingulate cortex. This needs to be recognised for adequate interpretation of IMP brain perfusion SPET after scatter correction. (orig.)

  4. Gas flow analysis during thermal vacuum test of a spacecraft. [self contamination of IMP spacecraft

    Science.gov (United States)

    Scialdone, J. J.

    1974-01-01

    The self-contamination of the IMP-H spacecraft, while it was undergoing thermal and solar vacuum tests, has been investigated in conjunction with the outgassing evaluation and detection of molecular flow anomalies occurring in the test chamber. The pressures indicated by two tubulated ionization gauges were used to calculate flow kinetics in the vacuum chamber. The fluxes of emitted molecules and chamber wall reflected molecules were monitored during the entire test. Representative equations and graphs are presented. Test results indicate that from 3 to 9 of every 100 emitted molecules returned to the spacecraft surface; that self-contamination by noncondensable gases was more severe than that by condensable gases; and that outgassing of the spacecraft was approximately 1.18 x 0.01 g/s after 10 hours and 1.18 x 0.001 after 90 hours of vacuum exposure. Testing deficiencies have been identified, and the type and location of instruments required to measure the outgassing, the degree of contamination, and return flow are discussed.

  5. Terrestrial kilometric radiation. III - Average spectral properties. [observations by IMP-6 and RAE-2 satellites

    Science.gov (United States)

    Kaiser, M. L.; Alexander, J. K.

    1977-01-01

    The spectral properties of terrestrial kilometric radiation (TKR) derived from observations made during radio-astronomy experiments on board the Imp 6 and Radio Astronomy Explorer 2 spacecraft are studied. As viewed from near the equatorial plane, TKR is most intense and most often observed in the 2100-2400 LT zone and is rarely seen in the 0900-1200 LT zone. The absolute flux levels in the 100- to 600-kHz TKR band increase significantly with increasing substorm activity as inferred from the auroral electrojet index (AE). In the late-evening sector the median power increases by about 3 orders of magnitude between quiet periods (AE less than 75 gammas) and disturbed periods (AE above 200 gammas). The peak flux density usually occurs near 250 kHz, although the frequency of the peak in the flux spectrum appears to vary inversely with AE from a maximum near 300 kHz during very quiet times to a minimum below 200 kHz during very disturbed times. The half-power bandwidth is typically 100% of the peak frequency. The variation of TKR flux density with apparent source altitude indicates that source strength decreases more rapidly than the inverse square of distance.

  6. La copla de moral sefardí Azote de impíos

    Directory of Open Access Journals (Sweden)

    Romero, Elena

    2010-06-01

    Full Text Available Three versions of the Sephardic “Copla de moral” Azote de Impios (‘Scourge of the Wicked’ are analyzed in this article. They were all printed in the nineteenth century, in Constantinople (1858 (version A, in Leipzig (1858 (version B and in Jerusalem (1894 (version C. The Version B was included in the German Nach Jerusalem! by Ludwig Aug. Frankl is of interest as it was written with Latin letters according to a special system that reflects the sounds of the original Judeo-Spanish.En el presente artículo se lleva a cabo la edición y estudio de las tres versiones conocidas de la copla de moral sefardí titulada Azote de impíos, impresas las dos primeras en 1858 en Constantinopla (versión A y Leipzig (versión B, respectivamente, y la tercera en Jerusalén en 1894 (versión C. La versión B, inserta en el libro alemán Nach Jerusalem! de Ludwig Aug. Frankl, presenta la particularidad de estar escrita en caracteres latinos siguiendo un muy especial sistema para plasmar los sonidos del judeoespañol.

  7. Development of single-event-effects analysis system at the IMP microbeam facility

    Science.gov (United States)

    Guo, Jinlong; Du, Guanghua; Bi, Jinshun; Liu, Wenjing; Wu, Ruqun; Chen, Hao; Wei, Junze; Li, Yaning; Sheng, Lina; Liu, Xiaojun; Ma, Shuyi

    2017-08-01

    Single-event-effects (SEEs) in integrated circuits (ICs) caused by galactic single ions are the major cause of anomalies for a spacecraft. The main strategies to decrease radiation failures for spacecraft are using SEEs less-sensitive devices and design radiation hardened ICs. High energy ion microbeam is one of the powerful tools to obtain spatial information of SEEs in ICs and to guide the radiation hardening design. The microbeam facility in the Institute of Modern Physics (IMP), Chinese Academy of Science (CAS) can meet both the liner energy transfer (LET) and ion range requirements for SEEs simulation experiments on ground. In order to study SEEs characteristics of ICs at this microbeam platform, a SEEs analysis system was developed. This system can target and irradiate ICs with single ions in micrometer-scale accuracy, meanwhile it acquires multi-channel SEE signals and maps the SEE sensitive regions online. A 4-Mbit NOR Flash memory was tested with this system using 2.2 GeV Kr ions, the radiation sensitive peripheral circuit regions for SEEs of 1 to 0 and 0 to 1 upset, multi-bit-upset and single event latchup have been obtained.

  8. Synthesis of a Phenylhydrazone-based Colorimetric Anion Sensor with Complementary IMP/INH Logic Functions

    Institute of Scientific and Technical Information of China (English)

    韦科; 熊碧; 周娜娜; 蔡苹; 胡锴; 程功臻

    2012-01-01

    A dinitrophenyl hydrazone colorimetric anion sensor (receptor 1) was synthesized and its recognition properties towards various anions were investigated by naked eye observation and spectroscopic methods, namely UV-vis and 1H NMR titrations in DMSO. The addition of AcO?, F? and H2PO4? to receptor 1 resulted in marked red shift of the charge-transfer absorbance band (Δλ=91 nm, 407 nm to 498 nm) concomitant with a 'naked-eye' detectable colour change from yellow to pink. However, both the colour and spectral changes were reversible by the addition of cations (MII) of 3d5-10 as well as CdII, HgII, MgII and CaII. Subsequently, complementary IMP/INH logic functions based on colour and spectral switching (ON/OFF) were affirmed. The sensor can, thus be utilized as a colorimetric molecular switch modulated by F?/MII.

  9. Sieviešu vara Osmaņu impērijā 17.-18.gs.

    OpenAIRE

    Krieķis, Jānis

    2017-01-01

    Bakalaura darba tēma ir “Sieviešu vara Osmaņu impērijā 17.-18.gs.” Darba uzdevums ir aplūkot vēsturisko situāciju un procesus Osmaņu impērijā, kas gala rezultātā noveda pie tā dēvētā “Sieviešu sultanāta”. Tas bija periods Osmaņu impērijas vēsturē, kad valsts politiskajos procesos ievērojamu lomu spēlēja tieši sievietes, neskatoties uz šķietami necilu lomu tradicionālajā islāma izpratnē par sievietēm. Darbs sastāv no trim daļām. Pirmajā daļā tiek apskatīts tradicionālais varas modelis un priek...

  10. Investigation of klebsiella pneumoniae-producing metallo-β-lactamases IMP-4%产金属β-内酰胺酶IMP-4肺炎克雷伯菌调查分析

    Institute of Scientific and Technical Information of China (English)

    韩秀峰; 苏旭; 井良义; 刘广文; 董杰; 王志锐; 朱韬; 穆祥弟

    2011-01-01

    OBJECTIVE To analyze production of metallo-β-1actamases (MBLs) and carbapenemase in one imipenem-resistant Klebsiella pneumoniae isolate. METHODS Two strains of K. pneumoniae were isolated from samples of urine and sputum respectively from female inpatient admitted to Tianjin Hongqiao hospital in Apr,2010. MIC method was used to perform the in vitro antimicrobial susceptibility testing for the isolates, and extended-spectrum beta-lactamase (ESBLs) production was tested for the isolates according to CLSI criteria. PCR were used to amplify MBLs genes, blaIMP and blaVIM, and KPC gene. PCR products were sequenced and analyzed. RESULTS The isolates showed multi-drug resistance. The isolate from urine (HQU) was resistant to imipenem and the isolate from sputum was not. Both of the isolates were producing ESBLs. K. pneumoniae HQU was carrying blaIMP-4 located in plasmid. The blaVIM and KPC genes were not detected out. CONCLUSION The presence of MBLs, IMP-4, in K. pneumoniae HQU highlights the continued spread of resistance determinants. It emphasizes the importance of surveillance programs and strict medication.%目的 对1株耐亚胺培南肺炎克雷伯菌(KPN)进行产金属β-内酰胺酶(MBLs)和产碳青霉烯酶(KPC)分析.方法 2010年4月2株KPN分别分离自天津市红桥医院1例住院患者的尿标本和痰标本,MIC法对分离菌株进行药物敏感试验,ESBLs确证试验检测其是否产ESBLs,PCR扩增blaIMP、blaVIM和KPC基因,扩增产物进行测序和分析.结果 2株KPN均为多药耐药菌,其中从尿中分离的KPN(HQU)对亚胺培南耐药,从痰中分离的KPN对亚胺培南敏感,均产ESBLs;从尿中分离的KPN携带MBLs基因blaIMP-4,且定位于质粒上;未检出blaVIM和KPC基因.结论产blaIMP-4型MBLs KPN的出现提示耐药性传播广泛,临床应加强耐药监测和严格药物使用.

  11. IMP 2.0: a multi-species functional genomics portal for integration, visualization and prediction of protein functions and networks.

    Science.gov (United States)

    Wong, Aaron K; Krishnan, Arjun; Yao, Victoria; Tadych, Alicja; Troyanskaya, Olga G

    2015-07-01

    IMP (Integrative Multi-species Prediction), originally released in 2012, is an interactive web server that enables molecular biologists to interpret experimental results and to generate hypotheses in the context of a large cross-organism compendium of functional predictions and networks. The system provides biologists with a framework to analyze their candidate gene sets in the context of functional networks, expanding or refining their sets using functional relationships predicted from integrated high-throughput data. IMP 2.0 integrates updated prior knowledge and data collections from the last three years in the seven supported organisms (Homo sapiens, Mus musculus, Rattus norvegicus, Drosophila melanogaster, Danio rerio, Caenorhabditis elegans, and Saccharomyces cerevisiae) and extends function prediction coverage to include human disease. IMP identifies homologs with conserved functional roles for disease knowledge transfer, allowing biologists to analyze disease contexts and predictions across all organisms. Additionally, IMP 2.0 implements a new flexible platform for experts to generate custom hypotheses about biological processes or diseases, making sophisticated data-driven methods easily accessible to researchers. IMP does not require any registration or installation and is freely available for use at http://imp.princeton.edu.

  12. The role of preclinical SPECT in oncological and neurological research in combination with either CT or MRI

    Energy Technology Data Exchange (ETDEWEB)

    Bernsen, Monique R.; Jong, Marion de [Erasmus MC, Department of Nuclear Medicine, Rotterdam (Netherlands); Erasmus MC, Department of Radiology, Rotterdam (Netherlands); Vaissier, Pieter E.B. [Delft University of Technology, Section Radiation Detection and Medical Imaging, Delft (Netherlands); Holen, Roel van [Ghent University, iMinds, ELIS Department, MEDISIP, Ghent (Belgium); Booij, Jan [University of Amsterdam, Department of Nuclear Medicine, Academic Medical Center, Amsterdam (Netherlands); Beekman, Freek J. [Delft University of Technology, Section Radiation Detection and Medical Imaging, Delft (Netherlands); MILabs B.V., Utrecht (Netherlands)

    2014-05-15

    Preclinical imaging with SPECT combined with CT or MRI is used more and more frequently and has proven to be very useful in translational research. In this article, an overview of current preclinical research applications and trends of SPECT combined with CT or MRI, mainly in tumour imaging and neuroscience imaging, is given and the advantages and disadvantages of the different approaches are described. Today SPECT and CT systems are often integrated into a single device (commonly called a SPECT/CT system), whereas at present combined SPECT and MRI is almost always carried out with separate systems and fiducial markers to combine the separately acquired images. While preclinical SPECT/CT is most widely applied in oncology research, SPECT combined with MRI (SPECT/MRI when integrated in one system) offers the potential for both neuroscience applications and oncological applications. Today CT and MRI are still mainly used to localize radiotracer binding and to improve SPECT quantification, although both CT and MRI have additional potential. Future technology developments may include fast sequential or simultaneous acquisition of (dynamic) multimodality data, spectroscopy, fMRI along with high-resolution anatomic MRI, advanced CT procedures, and combinations of more than two modalities such as combinations of SPECT, PET, MRI and CT all together. This will all strongly depend on new technologies. With further advances in biology and chemistry for imaging molecular targets and (patho)physiological processes in vivo, the introduction of new imaging procedures and promising new radiopharmaceuticals in clinical practice may be accelerated. (orig.)

  13. Comparison between stress myocardial perfusion SPECT recorded with cadmium-zinc-telluride and Anger cameras in various study protocols

    Energy Technology Data Exchange (ETDEWEB)

    Verger, Antoine; Karcher, Gilles [CHU-Nancy, Department of Nuclear Medicine, Nancy (France); INSERM U947, Nancy (France); Nancyclotep Experimental Imaging Platform, Nancy (France); Djaballah, Wassila [CHU-Nancy, Department of Nuclear Medicine, Nancy (France); INSERM U947, Nancy (France); Fourquet, Nicolas [Clinique Pasteur, Toulouse (France); Rouzet, Francois; Le Guludec, Dominique [AP-HP, Hopital Bichat, Department of Nuclear Medicine, Paris (France); INSERM U 773 Inserm and Denis Diderot University, Paris (France); Koehl, Gregoire; Roch, Veronique [CHU-Nancy, Department of Nuclear Medicine, Nancy (France); Nancyclotep Experimental Imaging Platform, Nancy (France); Imbert, Laetitia [CHU-Nancy, Department of Nuclear Medicine, Nancy (France); Nancyclotep Experimental Imaging Platform, Nancy (France); Centre Alexis Vautrin, Department of Radiotherapy, Vandoeuvre (France); Poussier, Sylvain [INSERM U947, Nancy (France); Nancyclotep Experimental Imaging Platform, Nancy (France); Fay, Renaud [INSERM, Centre d' Investigation Clinique CIC-P 9501, Nancy (France); Marie, Pierre-Yves [CHU-Nancy, Department of Nuclear Medicine, Nancy (France); Nancyclotep Experimental Imaging Platform, Nancy (France); INSERM U961, Nancy (France); Hopital de Brabois, CHU-Nancy, Medecine Nucleaire, Vandoeuvre-les-Nancy (France)

    2013-03-15

    The results of stress myocardial perfusion SPECT could be enhanced by new cadmium-zinc-telluride (CZT) cameras, although differences compared to the results with conventional Anger cameras remain poorly known for most study protocols. This study was aimed at comparing the results of CZT and Anger SPECT according to various study protocols while taking into account the influence of obesity. The study population, which was from three different institutions equipped with identical CZT cameras, comprised 276 patients referred for study using protocols involving {sup 201}Tl (n = 120) or {sup 99m}Tc-sestamibi injected at low dose at stress ({sup 99m}Tc-Low; stress/rest 1-day protocol; n = 110) or at high dose at stress ({sup 99m}Tc-High; rest/stress 1-day or 2-day protocol; n = 46). Each Anger SPECT scan was followed by a high-speed CZT SPECT scan (2 to 4 min). Agreement rates between CZT and Anger SPECT were good irrespective of the study protocol (for abnormal SPECT, {sup 201}Tl 92 %, {sup 99m}Tc-Low 86 %, {sup 99m}Tc-High 98 %), although quality scores were much higher for CZT SPECT with all study protocols. Overall correlations were high for the extent of myocardial infarction (r = 0.80) and a little lower for ischaemic areas (r = 0.72), the latter being larger on Anger SPECT (p < 0.001). This larger extent was mainly observed in 50 obese patients who were in the {sup 201}Tl or {sup 99m}Tc-Low group and in whom stress myocardial counts were particularly low with Anger SPECT (228 {+-} 101 kcounts) and dramatically enhanced with CZT SPECT (+279 {+-} 251 %). Concordance between the results of CZT and Anger SPECT is good regardless of study protocol and especially when excluding obese patients who have low-count Anger SPECT and for whom myocardial counts are dramatically enhanced on CZT SPECT. (orig.)

  14. Reference Range of Functional Data of Gated Myocardial Perfusion SPECT by Quantitative Gated SPECT of Cedars-Sinai and 4D-MSPECT of Michigan University

    Energy Technology Data Exchange (ETDEWEB)

    Kang, Do Young; Kim, Moo Hyun; Kim, Young Dae [College of Medicine, Univ. of Donga, Pusan (Korea, Republic of)

    2003-07-01

    Various programs have been developed for gating of myocardial perfusion SPECT. Among the those program, the most popular program is the Quantitative Gated SPECT (QGS)? developed by Cedars-Sinai hospital and most recently released program is 4D-MSPECT? developed by university of Michigan. It is important to know the reference range of the functional data of gated myocardial perfusion SPECT because it is necessary to determine abnormality of individual patient and echocardiographic data is different from those of gated SPECT. Tc-99m MIBI gated myocardial perfusion SPECT image was reconstructed by dual head gamma camera (Siemens, BCAM, esoft) as routine procedure and analyzed using QGS? and 4D-MSPECT? program. All patients (M: F=9: 18, Age 69{+-}9 yrs) showed normal myocardial perfusion. The patients with following characteristics were excluded: previous angina or MI history, ECG change with Q wave or ST-T change, diabetes melitius, hypercholesterolemia, typical chest pain, hypertension and cardiomyopathy. Pre-test likelihood of all patients was low. (1) In stress gated SPECT by QGS?, EDV was 73{+-}25 ml, ESV 25{+-}14 ml, EF 67{+-}11 % and area of first frame of gating 106.4{+-}21cm{sup 2}. In rest gated SPECT, EDV was 76{+-}26 ml, ESV 27{+-}15 ml, EF 66{+-}12 and area of first frame of gating 108{+-}20cm{sup 2}. (2) In stress gated SPECT by 4D-MSPECT?, EDV was 76{+-}28 ml, ESV 23{+-}16 ml, EF 72{+-}11 %, mass 115{+-}24 g and ungated volume 42{+-}15 ml. In rest gated SPECT, EDV was 75{+-}27 ml, ESV 23{+-}12 ml, EF 71{+-}9%, mass 113{+-}25g and ungate dvolume 42{+-}15 ml, (3) s-EDV, s-EF, r-ESV and r-EF were significantly different between QGS? and 4D-MSPECT? (each p=0.016, p<0.001. p=0.003 and p=0.001). We determined the normal reference range of functional parameters by QGS? and 4D-MSPECT? program to diagnose individually the abnormality of patients. And the reference ranges have to adopted to be patients by each specific gating program.

  15. Serial cerebral hemodynamic ch