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Sample records for hyptis martiusii benth

  1. Cytotoxic abietane diterpenes from Hyptis martiusii Benth.

    Science.gov (United States)

    da Araújo, Edigênia Cavalcante Cruz; Lima, Mary Anne S; Montenegro, Raquel C; Nogueira, Marcelle; Costa-Lotufo, Letícia V; Pessoa, Cláudia; de Moraes, Manoel Odorico; Silveira, Edilberto R

    2006-01-01

    From roots of Hyptis martiusii Benth. two tanshinone diterpenes were isolated, the new 7beta-hydroxy-11,14-dioxoabieta-8,12-diene (1) in addition to the known 7alpha-acetoxy-12-hydroxy-11,14-dioxoabieta-8,12-diene (7alpha-acetoxyroyleanone) (2). Structures of 1 and 2 were established by spectroscopic means. The cytotoxic activity against five cancer cell lines was evaluated. Compounds 1 and 2 displayed considerable cytotoxic activity against several cancer cell lines with IC50 values in the range of 3.1 to 11.5 microg/ml and 0.9 to 7.6 microg/ml, respectively. The cytotoxic activity seemed to be related to inhibition of DNA synthesis, as revealed by the reduction of 5-bromo-2'-deoxyuridine incorporation and induction of apoptosis, as indicated by the acridine orange/ethidium bromide assay and morphological changes after 24 h of incubation in leukemic cells.

  2. Spectral assignments of new diterpenes from Hyptis martiusii Benth.

    Science.gov (United States)

    da Cruz Araújo, Edigênia Cavalcante; Lima, Mary Anne Sousa; Silveira, Edilberto Rocha

    2004-12-01

    The structural characterization of two new abietanes and a new spiro-fused tricyclic diterpene isolated from the roots of Hyptis martiusii is described. The first member of a new class of rearranged abietane diterpenoids designated martiusane was characterized by the use of 1D NMR and several 2D shift correlated NMR pulse sequences (1H,1H-COSY, HMQC, HMBC and NOESY). Unambiguous 1H and 13C chemical shift assignments for all compounds are reported. Copyright 2004 John Wiley & Sons, Ltd.

  3. In vitro interference of Hyptis martiusii Benth. & chlorpromazine against an aminoglycoside-resistant Escherichia coli.

    Science.gov (United States)

    Coutinho, Henrique D M; Costa, José G M; Lima, Edeltrudes O; Falcão-Silva, Vivyanne S; Siqueira-Júnior, José P

    2009-05-01

    The antibacterial and synergistic activity of the ethanol extract from Hyptis martiusii Benth. was assayed by microdillution. The growth of two isolates of Escherichia coli tested was inhibited by the extract. The minimum inhibitory and minimum bactericidal concentrations (MIC and MBC) values ranged from 512 and >1024 microg/ml for the E. coli 27 and 1024 and > 1024 microg/ml for the E. coli ATCC8539, respectively. A synergism between this extract and all aminoglycosides assayed was demonstrated. In the same form synergism between chlorpromazine and kanamycin, amikacin and tobramycin was observed, indicating the involvement of an efflux system. Extracts from H. martiusii could be used as a source of plant derived natural products with modifying antibiotic activity and these products may interact and affect multidrug resistance systems (MDR) as efflux pumps.

  4. Gastroprotective and ulcer healing effects of essential oil of Hyptis martiusii Benth. (Lamiaceae).

    Science.gov (United States)

    Caldas, Germana Freire Rocha; Oliveira, Alisson Rodrigo da Silva; Araújo, Alice Valença; Quixabeira, Dafne Carolina Alves; Silva-Neto, Jacinto da Costa; Costa-Silva, João Henrique; de Menezes, Irwin Rose Alencar; Ferreira, Fabiano; Leite, Ana Cristina Lima; da Costa, José Galberto Martins; Wanderley, Almir Gonçalves

    2014-01-01

    Hyptis martiusii Benth. is an aromatic plant found in abundance in northeastern Brazil that is used in ethnomedicine to treat gastric disorders. The aim of this study was to elucidate the mechanisms of action involved in the gastroprotection of the essential oil of Hyptis martiusii (EOHM) and to evaluate its healing capacity. Wistar rats were exposed to different protocols and subsequently were treated with 1% Tween-80 aqueous solution (negative control), pantoprazole, carbenoxolone, N-acetylcysteine (depending on the specificity of each model) or EOHM. The antisecretory activity (basal or stimulated) was determined using the pyloric ligature method. The gastroprotective action of nitric oxide and sulphydryl groups (-SH groups), as well as the quantification of adherent mucus and the levels of malondialdehyde and -SH groups in gastric mucosa, were evaluated using ethanol-induced gastric ulcer model. The healing ability was evaluated using the acetic acid-induced gastric ulcer model and histological and immunohistochemical analysis (HE, PAS and PCNA). EOHM (400 mg/kg) reduced the volume and acidity of gastric secretion stimulated by histamine and pentagastrin. The gastroprotective effect of EOHM involves the participation of endogenous sulfhydryl groups. EOHM increased mucus production (54.8%), reduced levels of MDA (72.5%) and prevented the depletion of -SH groups (73.8%) in the gastric mucosa. The treatment with EOHM reduced in 70.3% the gastric lesion area, promoting significant regeneration of the gastric mucosa, as confirmed by histological analysis and analysis of proliferating cell nuclear antigen. The results show that gastroprotective effect of EOHM is mediated by cytoprotective and antioxidant mechanisms and by their antisecretory activity, and suggest that the essential oil of Hyptis martiusii is a promising candidate for the treatment of gastric ulcers.

  5. Gastroprotective and ulcer healing effects of essential oil of Hyptis martiusii Benth. (Lamiaceae.

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    Germana Freire Rocha Caldas

    Full Text Available Hyptis martiusii Benth. is an aromatic plant found in abundance in northeastern Brazil that is used in ethnomedicine to treat gastric disorders. The aim of this study was to elucidate the mechanisms of action involved in the gastroprotection of the essential oil of Hyptis martiusii (EOHM and to evaluate its healing capacity. Wistar rats were exposed to different protocols and subsequently were treated with 1% Tween-80 aqueous solution (negative control, pantoprazole, carbenoxolone, N-acetylcysteine (depending on the specificity of each model or EOHM. The antisecretory activity (basal or stimulated was determined using the pyloric ligature method. The gastroprotective action of nitric oxide and sulphydryl groups (-SH groups, as well as the quantification of adherent mucus and the levels of malondialdehyde and -SH groups in gastric mucosa, were evaluated using ethanol-induced gastric ulcer model. The healing ability was evaluated using the acetic acid-induced gastric ulcer model and histological and immunohistochemical analysis (HE, PAS and PCNA. EOHM (400 mg/kg reduced the volume and acidity of gastric secretion stimulated by histamine and pentagastrin. The gastroprotective effect of EOHM involves the participation of endogenous sulfhydryl groups. EOHM increased mucus production (54.8%, reduced levels of MDA (72.5% and prevented the depletion of -SH groups (73.8% in the gastric mucosa. The treatment with EOHM reduced in 70.3% the gastric lesion area, promoting significant regeneration of the gastric mucosa, as confirmed by histological analysis and analysis of proliferating cell nuclear antigen. The results show that gastroprotective effect of EOHM is mediated by cytoprotective and antioxidant mechanisms and by their antisecretory activity, and suggest that the essential oil of Hyptis martiusii is a promising candidate for the treatment of gastric ulcers.

  6. Insecticidal activity and chemical composition of volatile oils from Hyptis martiusii Benth.

    Science.gov (United States)

    Araújo, Edigênia C C; Silveira, Edilberto R; Lima, Mary Anne S; Neto, Manoel Andrade; de Andrade, Israel L; Lima, Marcos Aurélio A; Santiago, Gilvandete M P; Mesquita, Antonio Lindemberg M

    2003-06-18

    The essential oils from leaves and inflorescences of Hyptis martiusii Benth were analyzed by GC-MS. Twenty-six compounds representing 93.2% of the essential oil of leaves were characterized; Delta-3-carene (22.5%), 1,8-cineole (24.27%), beta-caryophyllene (6.15%), and bicyclogermacrene (6.32%) were found as the major components. In the essential oil of inflorescences 27 compounds representing 87.7% of the oil were identified. The major components were Delta-3-carene (13.5%), alpha-pinene (5.78%), beta-caryophyllene (6.59%), viridiflorene (8.25%), and germacrene B (5.21%). The essential oil of leaves and 1,8-cineole showed pronounced insecticidal effect against Aedes aegypti larvae and Bemisia argentifolii, the vectors of dengue fever and white fly fruit plague, respectively.

  7. Antiulcerogenic activity of the essential oil of Hyptis martiusii Benth. (Lamiaceae)

    National Research Council Canada - National Science Library

    Caldas, Germana Freire Rocha; do Amaral Costa, Iggor Macêdo; da Silva, Juciene Bezerra Rodrigues; da Nóbrega, Rafaella Farias; Rodrigues, Fabíola Fernandes Galvão; da Costa, José Galberto Martins; Wanderley, Almir Gonçalves

    2011-01-01

    Hyptis martiusii (Lamiaceae), popularly known as "cidreira-do-mato" or "cidreira-brava", grows in abundance in the Northeast region of Brazil, where its leaves are traditionally used in folk medicine in the treatment...

  8. Evaluation of chemical composition and antiedematogenic activity of the essential oil of Hyptis martiusii Benth.

    Science.gov (United States)

    Barbosa, Andreza G R; Oliveira, Cicera D M; Lacerda-Neto, Luiz J; Vidal, Cinara S; Saraiva, Rogério de A; da Costa, José G M; Coutinho, Henrique D M; Galvao, Hericka B F; de Menezes, Irwin R A

    2017-02-01

    Evaluations of the therapeutic potential of medicinal plants and their components have been the subject of many studies. Furthermore, the biological activities of various plant species have been reported in various pieces of literature. Hyptis martiusii Benth (Lamiaceae), popularly known as "mad balm" is commonly found in the North, Southeast, and Northeast of Brazil. Its leaves are used ethnobiologically as antiulcerogenic, antimicrobial, antitumor and as insecticide. This study aimed to analyze the chemical composition of the essential oil of H. martiusii Benth (OEHM) by GC/MS as well as its possible topical activity as an antiedematogenic. This is verified by the models of ear edema induced by single (acute edema) and multiple (chronic edema) applications of croton oil topically, and systemically verified through the model of paw edema induced by carrageenan 1%. Doses of 50, 75 and 100 mg/kg OEHM were used in all tests. Chemical analysis of the oil revealed the 1,8-cineole (34.58%) and δ-carene (21:58%) as major components present in the essential oil. On the model of ear edema, acute and chronic OEHM in all the tested doses showed no significant antiedematogenic activity (p < 0.05). The systemic model of paw edema induced by carrageenin showed that a dose of 100 mg/kg effectively reduced swelling by 55.37% in the second hour evaluation when compared to the saline group. The anti-inflammatory systemic effect can give greater bioavailability of the components present in the essential oil and your interference in cytokines and leukotriene, thromboxane and prostaglandin biosynthesis. It is therefore concluded that OEHM presents systemic antiedematogenic activity but not topical activity at these doses.

  9. Evaluation of chemical composition and antiedematogenic activity of the essential oil of Hyptis martiusii Benth

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    Andreza G.R. Barbosa

    2017-02-01

    Full Text Available Evaluations of the therapeutic potential of medicinal plants and their components have been the subject of many studies. Furthermore, the biological activities of various plant species have been reported in various pieces of literature. Hyptis martiusii Benth (Lamiaceae, popularly known as “mad balm” is commonly found in the North, Southeast, and Northeast of Brazil. Its leaves are used ethnobiologically as antiulcerogenic, antimicrobial, antitumor and as insecticide. This study aimed to analyze the chemical composition of the essential oil of H. martiusii Benth (OEHM by GC/MS as well as its possible topical activity as an antiedematogenic. This is verified by the models of ear edema induced by single (acute edema and multiple (chronic edema applications of croton oil topically, and systemically verified through the model of paw edema induced by carrageenan 1%. Doses of 50, 75 and 100 mg/kg OEHM were used in all tests. Chemical analysis of the oil revealed the 1,8-cineole (34.58% and δ-carene (21:58% as major components present in the essential oil. On the model of ear edema, acute and chronic OEHM in all the tested doses showed no significant antiedematogenic activity (p < 0.05. The systemic model of paw edema induced by carrageenin showed that a dose of 100 mg/kg effectively reduced swelling by 55.37% in the second hour evaluation when compared to the saline group. The anti-inflammatory systemic effect can give greater bioavailability of the components present in the essential oil and your interference in cytokines and leukotriene, thromboxane and prostaglandin biosynthesis. It is therefore concluded that OEHM presents systemic antiedematogenic activity but not topical activity at these doses.

  10. In vitro anti-staphylococcal activity of Hyptis martiusii Benth against methicillin-resistant Staphylococcus aureus: MRSA strains

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    Henrique D. M. Coutinho

    Full Text Available This is the first report about the antibacterial activity of Hyptis martiusii Benth. In this study the ethanol extract of H. martiusii was tested for its antimicrobial activity against strains of Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus. The growth of all bacterial strains tested was inhibited by the extract. The diameter of inhibition zones varied from 13 to 20 mm for the extract. The MIC and MBC values ranged from 128 to > 1024mg/mL and 256 to > 1024 mg/mL, respectively. It is therefore suggested that extracts from H. martiusii could be used as an anti-Staphylococcus agent. Compared with methicillin and gentamicin, the extract was more effective, being a promising antibacterial agent.

  11. In vitro phototoxic activity of Eugenia jambolana L. and Hyptis martiusii Benth.

    Science.gov (United States)

    Coutinho, Henrique D M; Costa, José G M; Lima, Edeltrudes O; Siqueira-Júnior, José P

    2009-07-17

    Ethanol extracts from Hyptis martiusii and Eugenia jambolana were assayed for light-mediated activity against strains of Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa. Duplicate assays were conducted with and without exposure to UV-A radiation to test for light-activated or light-enhanced activity. Both extracts showed activity against at least two bacterial strains, but H. martiusii was the most active extract, being active against all strains of S. aureus and E. coli. The results represent a first report of the light-mediated antimicrobial activities of H. martiusii and E. jambolana and suggest that phytochemical investigations may be warranted.

  12. Repeated-Doses Toxicity Study of the Essential Oil of Hyptis martiusii Benth. (Lamiaceae) in Swiss Mice.

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    Freire Rocha Caldas, Germana; Araújo, Alice Valença; Albuquerque, Giwellington Silva; Silva-Neto, Jacinto da Costa; Costa-Silva, João Henrique; de Menezes, Irwin Rose Alencar; Leite, Ana Cristina Lima; da Costa, José Galberto Martins; Wanderley, Almir Gonçalves

    2013-01-01

    Hyptis martiusii Benth. (Lamiaceae) is found in abundance in Northeastern Brazil where it is used in traditional medicine to treat gastric disorders. Since there are no studies reporting the toxicity and safety profile of this species, we investigated repeated-doses toxicity of the essential oil of Hyptis martiusii (EOHM). Swiss mice of both sexes were orally treated with EOHM (100 and 500 mg/kg) for 30 days, and biochemical, hematological, and morphological parameters were determined. No toxicity signs or deaths were recorded during the treatment with EOHM. The body weight gain was not affected, but there was an occasional variation in water and food consumption among mice of both sexes treated with both doses. The hematological and biochemical profiles did not show significant differences except for a decrease in the MCV and an increase in albumin, but these variations are within the limits described for the species. The microscopic analysis showed changes in liver, kidneys, lungs, and spleen; however, these changes do not have clinical relevance since they varied among the groups, including the control group. The results indicate that the treatment of repeated-doses with the essential oil of Hyptis martiusii showed low toxicity in mice.

  13. Repeated-Doses Toxicity Study of the Essential Oil of Hyptis martiusii Benth. (Lamiaceae in Swiss Mice

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    Germana Freire Rocha Caldas

    2013-01-01

    Full Text Available Hyptis martiusii Benth. (Lamiaceae is found in abundance in Northeastern Brazil where it is used in traditional medicine to treat gastric disorders. Since there are no studies reporting the toxicity and safety profile of this species, we investigated repeated-doses toxicity of the essential oil of Hyptis martiusii (EOHM. Swiss mice of both sexes were orally treated with EOHM (100 and 500 mg/kg for 30 days, and biochemical, hematological, and morphological parameters were determined. No toxicity signs or deaths were recorded during the treatment with EOHM. The body weight gain was not affected, but there was an occasional variation in water and food consumption among mice of both sexes treated with both doses. The hematological and biochemical profiles did not show significant differences except for a decrease in the MCV and an increase in albumin, but these variations are within the limits described for the species. The microscopic analysis showed changes in liver, kidneys, lungs, and spleen; however, these changes do not have clinical relevance since they varied among the groups, including the control group. The results indicate that the treatment of repeated-doses with the essential oil of Hyptis martiusii showed low toxicity in mice.

  14. Chemical Composition, Modulatory Bacterial Resistance and Antimicrobial Activity of Essential Oil the Hyptis martiusii Benth by Direct and Gaseous Contact.

    Science.gov (United States)

    de Oliveira, Allan Demetrius Leite; Galvao Rodrigue, Fabiola Fernandes; Douglas Melo Coutinho, Henrique; da Costa, Jose Galberto Martins; de Menezes, Irwin Rose Alencar

    2014-08-01

    Several studies have shown that species of the genus Hyptis, have promising antimicrobial and antifungal effects. Identify of chemical constituents of essential oil from leaves of Hyptis martiusii and evaluate its effect against bacterial strains by direct and gaseous contact. Essential oil was extracted from leaves of Hyptis martiusii Benth using hydro-distillation, and its composition was determined using gas chromatography-mass spectrometry (GC-MS). Chemical analysis showed that there was a predominance of sesquiterpenes. The leaf essential oil was screened for its minimal inhibitory concentration and modulatory effect of aminoglycoside by the direct (MIC) and gaseous (MID) micro-dilution assays for various pathogenic microorganisms. The essential oil remarkably inhibited the growth of all of the tested bacteria (MIC essential oil of leaves were characterized; δ -3-carene (6.88%), 1, 8-cineole (7.01%), trans-caryophyllene (9.21%), Cariophyllene oxide (7.47%) and bicyclogermacrene (10.61%) were found as the major components. Modulatory aminoglycoside effect, by direct contact, was showed antagonistic relationship with antimicrobial activity. The gaseous component of the oil inhibited the bacterial growth of all of the tested bacteria in 50% and 25% of oil concentration and demonstrated synergistic interactions can be attributed to the constituting the oil compounds. These results show that this oil influences the activity of the antibiotic and may be used as an adjuvant in the antibiotic therapy of respiratory tract bacterial pathogens.

  15. Antiulcerogenic activity of the essential oil of Hyptis martiusii Benth. (Lamiaceae).

    Science.gov (United States)

    Caldas, Germana Freire Rocha; do Amaral Costa, Iggor Macêdo; da Silva, Juciene Bezerra Rodrigues; da Nóbrega, Rafaella Farias; Rodrigues, Fabíola Fernandes Galvão; da Costa, José Galberto Martins; Wanderley, Almir Gonçalves

    2011-09-01

    Hyptis martiusii (Lamiaceae), popularly known as "cidreira-do-mato" or "cidreira-brava", grows in abundance in the Northeast region of Brazil, where its leaves are traditionally used in folk medicine in the treatment of intestinal and stomach diseases. The aim of this study was to characterize the chemical constituents and to evaluate the anti-ulcerogenic activity of the essential oil of the leaves of Hyptis martiusii (EOHM) in in vivo models of experimental ulcers in rodents. EOHM was obtained by hydrodistillation and analyzed by gas chromatography-mass spectrometry (GC-MS). Acute gastric ulcer was induced using absolute ethanol, HCl/ethanol and indomethacin. The volume, pH and total acidity of gastric secretion were determined by the pyloric ligature method and gastrointestinal motility using gastric emptying and intestinal transit. Chemical analysis revealed the presence of 24 components that account for 92.13% of the essential oil of dried leaves, indicating the occurrence of mono and sesquiterpenes. Oral administration of EOHM (100, 200 and 400mg/kg) inhibited ethanol-, HCl/ethanol- and indomethacin-induced ulcers. In the case of pylorus ligature, the oil reduced the volume of gastric juices and total acidity, and increased gastric pH. The EOHM reduced the rate of gastric emptying with only the highest doses, but did not show any effect on intestinal transit at any of the three doses. The results indicate that the essential oil of leaves of Hyptis martiusii has an antiulcerogenic activity, as evidenced by its significant inhibition of the formation of ulcers in various models. This effect could be related to an increase of gastric mucosal defensive factors. Further pharmacological studies are being undertaken in order to provide more precise elucidation of the action mechanism involved in this activity. Copyright © 2011 Elsevier Ireland Ltd. All rights reserved.

  16. Antiproliferative effects of abietane diterpenoids isolated from Hyptis martiusii Benth (Labiatae).

    Science.gov (United States)

    Costa-Lotufo, L V; Araújo, E C C; Lima, M A S; Moraes, M E A; Pessoa, C; Silviera, E R; Moraes, M O

    2004-01-01

    Two abietane diterpenes were isolated from a hexane extract of Hyptis martiusii roots and identified as carnasol 11,14-dihidroxy-8,11,13-abietatrien-7-one. These compounds were tested for their antiproliferative effects on tumor cell lines using 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide and on the sea urchin egg development. Both compounds displayed cytotoxic activity against tumor cell lines, but only carnasol was able to inhibit the sea urchin egg cleavages.

  17. Physico-chemical characterization and antibacterial activity of inclusion complexes of Hyptis martiusii Benth essential oil in β-cyclodextrin.

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    Andrade, Tatianny A; Freitas, Thiago S; Araújo, Francielly O; Menezes, Paula P; Dória, Grace Anne A; Rabelo, Alessandra S; Quintans-Júnior, Lucindo J; Santos, Márcio R V; Bezerra, Daniel P; Serafini, Mairim R; Menezes, Irwin Rose A; Nunes, Paula Santos; Araújo, Adriano A S; Costa, Maria S; Campina, Fábia F; Santos, Antonia T L; Silva, Ana R P; Coutinho, Henrique D M

    2017-05-01

    Cyclodextrins (CDs) have been used as important pharmaceutical excipients for improve the physicochemical properties of the drugs of low solubility as the essential oil of Hyptis martiusii. This oil is important therapeutically, but the low solubility and bioavailability compromises your use. Therein, the aim of this study was to obtain and to characterize physico-chemically the samples obtained by physical mixture (PM), paste complexation (PC) and slurry complexation (SC) of the essential oil Hyptis martiusii (EOHM) in β-CD, and to compare the antibacterial and modulatory-antibiotic activity of products obtained and oil free. The physicochemical characterization was performed by differential scanning calorimetry (DSC), thermogravimetry/derivative thermogravimetry (TG/DTG), scanning electron microscopy (SEM), X-ray diffraction (XRD) and Karl Fischer titration. Additionally, the antibacterial tests were performed by microdilution technique. Thus, it was observed that the PM method showed low complexing capacity, unlike PC and SC in which it was observed the formation of inclusion complexes. In addition, the second stage of the TG/DTG curves showed that SC was the best method inclusion with mass loss of 6.9% over the PC that was 6.0%. The XRD results corroborate with the results above suggesting the formation of new solid phase and the SEM photomicrographs showed the porous surface of the samples PC and SC. The essential oil alone demonstrated an antibacterial and modulatory effect against the S. aureus and the Gram negative strain, respectively. However, the β-CD and the inclusion complex did not demonstrate any biological activity in the performed antibacterial assays. Copyright © 2017 Elsevier Masson SAS. All rights reserved.

  18. In vitro additive effect of Hyptis martiusii in the resistance to aminoglycosides of methicillin-resistant Staphylococcus aureus.

    Science.gov (United States)

    Coutinho, Henrique D M; Costa, José G M; Falcão-Silva, Vivyanne S; Siqueira-Júnior, José P; Lima, Edeltrudes O

    2010-09-01

    Bacterial infectious agents represent a risk to populations, where they are responsible for the high morbidity and mortality. In combating these pathogens, our main line of defense is the use of antibiotics. However, the indiscriminate use of these drugs select resistant strains to these same drugs. In this study the ethanol extract of Hyptis martiusii Benth. (EEHM) (Lamiaceae) was tested for its antimicrobial activity against aminoglycoside multi-resistant Staphylococcus aureus (MRSA). In this study, the ethanol extract of H. martiusii was prepared and tested with chlorpromazine for its antimicrobial activity using the microdilution method. Chlorpromazine and the ethanol extract were used alone and also in combination with aminoglycosides against a MRSA strain resistant to these antibiotics to determine the participation of efflux systems in resistance mechanisms. The FIC index was calculated and evaluated by the checkerboard method. A potentiating effect between this extract and aminoglycosides was demonstrated. Similarly, a potentiating effect of chlorpromazine with kanamycin was detected, indicating the involvement of an efflux system in the resistance to this aminoglycoside. The checkerboard method with combinations of aminoglycosides and EEHM demonstrated additive effect with kanamycin and gentamicin. It is therefore suggested that extracts from H. martiusii could be used as a source of plant-derived natural products with resistance- modifying activity. This is the first report about the modifying antibiotic activity of Hyptis martiusii, constituting a new approach against bacterial resistance to antibiotics as aminoglycosides.

  19. Triterpenos de Hyptis fasciculata Benth.

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    D.Q. Falcão

    Full Text Available Partes aéreas do arbusto Hyptis fasciculata Benth. foram estudadas do ponto de vista químico. Após vários processos cromatográficos em coluna de gel de sílica, foi possível isolar um triterpeno livre (ácido betulínico, além de triterpenos em mistura (ácido ursólico com ácido betulínico e ácido ursólico com ácido oleanólico, esteróides em mistura (sitosterol e estigmasterol e misturas de estigmasterol com ácido caféico e sitosterol com lignana. Tais substâncias foram identificadas por ressonância magnética nuclear de 1H e 13C.

  20. Triterpenos de Hyptis fasciculata Benth.

    OpenAIRE

    Falcão, D.Q.; Fernandes, S.B.O.; Menezes, F.S.

    2003-01-01

    Partes aéreas do arbusto Hyptis fasciculata Benth. foram estudadas do ponto de vista químico. Após vários processos cromatográficos em coluna de gel de sílica, foi possível isolar um triterpeno livre (ácido betulínico), além de triterpenos em mistura (ácido ursólico com ácido betulínico e ácido ursólico com ácido oleanólico), esteróides em mistura (sitosterol e estigmasterol) e misturas de estigmasterol com ácido caféico e sitosterol com lignana. Tais substâncias foram identificadas por resso...

  1. Propagação vegetativa de Hyptis leucocephala Mart. ex Benth. e Hyptis platanifolia Mart. ex Benth. (Lamiaceae Vegetative propagation of Hyptis leucocephala Mart. ex Benth. and Hyptis platanifolia Mart. ex Benth

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    L.M Oliveira

    2011-01-01

    Full Text Available O gênero Hyptis (Lamiaceae apresenta metabolismo especial de notável variabilidade, possuindo predominância de óleos essenciais, que têm muito valor junto a diversas comunidades que utilizam essas plantas pelas propriedades terapêuticas. Diversos estudos com esse gênero têm demonstrado que os óleos essenciais apresentam propriedades antimicrobiana, antifúngica, citotóxica, antiinflamatória, anti-HIV e inseticida, entretanto, poucos são os estudos voltados para a exploração sustentável dessas espécies. O objetivo desse trabalho foi avaliar o efeito de diferentes tipos de estaca e diferentes concentrações de ácido indolbutírico (AIB sobre a taxa de sobrevivência, crescimento e enraizamento de estacas de Hyptis leucocephala Mart. ex Benth. e Hyptis platanifolia Mart. ex Benth., espécies endêmicas do semi-árido nordestino cujo valor medicinal tem sido amplamente comprovado. O experimento teve duração de quatro meses. Foram utilizadas estacas apicais, médio-apicais, médio-basais e basais tratadas com soluções de 0, 1000, 2000 e 4000 mg L-1 de ácido indolbutírico (AIB, durante 5 minutos. A utilização de estacas apicais, médio/apicais e médio/basais induzidas com 2000 e 4000 mg L-1 de AIB possibilitaram melhores resultados para propagação vegetativa de Hyptis leucocephala. Para Hyptis platanifolia os melhores resultados foram obtidos com a utilização de estacas médio/basais e basais tratadas com AIB na concentração de 2000 e 4000 mg L-1.The genus Hyptis (Lamiaceae has a special metabolism with remarkable variability and predominance of essential oils of great value to the various communities that use these plants due to their therapeutic properties. A number of studies on this genus have demonstrated that its essential oils present antimicrobial, antifungal, cytotoxic, anti-inflammatory, anti-HIV and insecticidal activities. However, few studies have focused on the sustainable exploitation of these species

  2. Estudo químico-biológico dos óleos essenciais de Hyptis martiusii, Lippia sidoides e Syzigium aromaticum frente às larvas do Aedes aegypti

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    J.G.M. Costa

    Full Text Available O interesse pelos bioensaios frente à larvas de Aedes aegypti e Culex quinquefasciatus deve-se ao fato de que estas espécies estão distribuídas por todo o território nacional, sendo portanto uma atividade realizada por inúmeros pesquisadores do Brasil. Os óleos essenciais de Syzigium aromaticum (L. Merr. & Perry, Lippia sidoides Cham.,e Hyptis martiusii Benth.,foram testados no combate ao transmissor da dengue e da filariose. As larvas de terceiro estádio foram expostas em triplicatas a diferentes concentrações (1000, 500, 250, 100, 50, 25 e 10 ppm. As análises foram observadas após dez minutos do início do tratamento, e mostraram resultados bastante significativos, com potencialidade de mortalidade de até 100% das larvas testadas, indicando acentuados efeitos tóxicos de alguns constituintes voláteis presentes nos óleos. Para os óleos de S. aromaticum, L. sidoides e H. martiusii foram constatadas, frente à Aedes aegypti, valores respectivos de CL50 de 21,4; 19,5 e 18,5 ppm e frente ao Culex quinquefasciatus, 14,5; 16,6 e 27,5 ppm, respectivamente.

  3. Vegetative propagation of Hyptis leucocephala Mart. ex Benth. and Hyptis platanifolia Mart. ex Benth

    OpenAIRE

    DE OLIVEIRA, L M; Nepomuceno, C.F; Freitas,N.P; Pereira,D.M.S; Silva, G.C; Lucchese, A.M

    2011-01-01

    O gênero Hyptis (Lamiaceae) apresenta metabolismo especial de notável variabilidade, possuindo predominância de óleos essenciais, que têm muito valor junto a diversas comunidades que utilizam essas plantas pelas propriedades terapêuticas. Diversos estudos com esse gênero têm demonstrado que os óleos essenciais apresentam propriedades antimicrobiana, antifúngica, citotóxica, antiinflamatória, anti-HIV e inseticida, entretanto, poucos são os estudos voltados para a exploração sustentável dessas...

  4. Contribuição ao conhecimento químico de espécies de Hyptis com ocorrência no semiárido paraibano: Hyptis macrostrachys Benth. e Hyptis umbrosa Salzm. Ex Benth.

    OpenAIRE

    Costa, Vicente Carlos de Oliveira

    2013-01-01

    A família Lamiaceae possui 295 gêneros e cerca de 7.775 espécies com distribuição cosmopolita. Estima-se que 22 gêneros e 402 espécies ocorrem no Brasil. Neste trabalho, realizou-se um estudo fitoquímico de duas espécies do gênero Hyptis encontradas no semiárido paraibano, Hyptis macrostachys Benth. e Hyptis umbrosa Salzm. ex Benth. As folhas e os caules das espécies em estudo foram submetidas a processos de extração, de filtração e cromatografia de seus respectivos extratos. Posteriormente, ...

  5. A new bis-seco-abietane diterpenoid from Hyptis crenata Pohl ex Benth.

    Science.gov (United States)

    Yun, Young Sook; Fukaya, Haruhiko; Nakane, Takahisa; Takano, Akihito; Takahashi, Shigeru; Takahashi, Yuji; Inoue, Hideshi

    2014-12-05

    A new, highly oxidized, bis-seco-abietane diterpenoid named hyptisolide A (1) was isolated from Hyptis crenata Pohl ex Benth. Its structure and stereochemistry were elucidated on the basis of data obtained by HRESIMS, NMR, and X-ray diffraction analyses, and its absolute configuration was determined with vibrational circular dichroism spectroscopy. By reporter gene assay, 1 was demonstrated to induce cAMP-responsive element-dependent transcription in Neuro2A cells.

  6. Chemical composition and antifungal activity of the essential oil of Hyptis ovalifolia Benth. (Lamiaceae)

    OpenAIRE

    Oliveira,Cecília M. A. de; Silva,Maria do Rosário R; Kato, Lucília; Silva,Cleuza C. da; Heleno D. Ferreira; Souza,Lúcia K. H.

    2004-01-01

    The leaves of Hyptis ovalifolia Benth. (Lamiaceae) were subjected to hydrodistillation and the resulting volatiles were investigated by GC/MS. The main constituent representing 60% of the essential oil was isolated by column chromatography and identified by spectroscopic methods as (R)-6-[(Z)-1-heptenyl]-5,6-dihydro-2H-pyran-2-one (1). This compound showed strong in vitro activity against four dermatophyte fungi Microsporum canis, Microsporum gypseum, Tricophyton mentagrophytes, and Tricophyt...

  7. Chemical composition and antifungal activity of the essential oil of Hyptis ovalifolia Benth. (Lamiaceae)

    Energy Technology Data Exchange (ETDEWEB)

    Oliveira, Cecilia M.A. de; Kato, Lucilia [Goias Univ., Goiania, GO (Brazil). Inst. de Quimica]. E-mail: cecilia@quimica.ufg.br; Silva, Maria do Rosario R.; Souza, Lucia K.H. [Goias Univ., Goiania, GO (Brazil). Inst. de Patologia Tropical; Silva, Cleuza C. da [Universidade Estadual de Maringa, PR (Brazil). Dept. de Quimica; Ferreira, Heleno D. [Goias Univ., Goiania, GO (Brazil). Inst. de Ciencias Biologicas

    2004-10-01

    The leaves of Hyptis ovalifolia Benth. (Lamiaceae) were subjected to hydro distillation and the resulting volatiles were investigated by GC/MS. The main constituent representing 60% of the essential oil was isolated by column chromatography and identified by spectroscopic methods as (R)-6-[(Z)-1-heptenyl]-5,6-dihydro-2H-pyran-2-one (1). This compound showed strong in vitro activity against four dermatophyte fungi Microsporum canis, Microsporum gypseum, Tricophyton mentagrophytes, and Tricophyton rubrum (a total of 60 strains) with a minimal inhibitory concentration observed in the range of 125-7.8 mg {mu}L{sup -1}. (author)

  8. Germinação in vitro de Hyptis leucocephala Mart. ex Benth. e Hyptis platanifolia Mart. ex Benth.

    Directory of Open Access Journals (Sweden)

    C.F Nepomuceno

    2014-12-01

    Full Text Available Hyptis leucocephala e Hyptis platanifolia (Lamiaceae são espécies aromáticas endêmicas do semiárido nordestino e possuem grande importância econômica devido ao seu potencial medicinal. O presente trabalho teve por objetivo obter o protocolo para o estabelecimento inicial in vitro das espécies H. leucocephala e H. platanifolia. O meio de cultura WPM promoveu a maior porcentagem de germinação (96,81% para H. leucocephala, porém não diferiu estatisticamente dos resultados obtidos no MS½ na última semana de cultivo. Para a germinação de sementes de H. platanifolia, o MS½ foi o meio que proporcionou a maior porcentagem (40,83%. O tipo de esterilização não interferiu na germinação in vitro para as duas espécies. Para a espécie H. platanifolia, quando se utilizou o fechamento dos tubos de ensaio com tampão de algodão, a hiperidricidade foi eliminada; porém, a porcentagem de germinação das sementes foi menor. Avaliando-se o tipo de meio de cultura no crescimento in vitro de H. leucocephala observou-se que este fator não influenciou no número de brotações, nem o comprimento de parte aérea e da matéria seca de raiz. O meio MS proporcionou incremento para o número de folhas, matéria seca da parte aérea, e comprimento da maior raiz, enquanto o meio MS½ foi o que promoveu maior número de raízes. O meio de cultura ideal para a germinação de H. leucocephala e H. platanifolia foi o MS½, podendo ser esterilizado com hipoclorito de sódio, enquanto que o MS foi o melhor meio de cultura para o crescimento in vitro de H. leucocephala.

  9. Gastroprotective effects of the essential oil of Hyptis crenata Pohl ex Benth. on gastric ulcer models.

    Science.gov (United States)

    Diniz, Lúcio Ricardo Leite; Vieira, Charliene Freire Xavier; Santos, Edgleyson Chaves Dos; Lima, Glauber Cruz; Aragão, Kalynca Kayla Viana; Vasconcelos, Renata Prado; Araújo, Pâmella Cristina da Costa; Vasconcelos, Yuri de Abreu Gomes; Oliveira, Ariclécio Cunha de; Oliveira, Hermógenes David de; Portella, Viviane Gomes; Coelho-de-Souza, Andrelina Noronha

    2013-10-07

    Hyptis crenata Pohl ex Benth (Lamiaceae), popularly known as "hortelã-brava" or "hortelã do campo", is widely distributed in the northeast of Brazil. In Brazil, the leaves of this plant have been used in traditional medicine for the treatment of gastrointestinal disturbances, including gastric ulcers. In an attempt to experimentally validate this claimed antiulcerogenic activity, the gastroprotective effects of the essential oil extracted from the leaves of the Hyptis crenata Pohl ex Benth (EOHc) were evaluated in recognized gastric ulcer models in mice. EOHc was obtained by hydrodistillation and analyzed using gas chromatography-mass spectrometry (GC-MS). Swiss male mice (25-30g) were used for the studies. The gastric ulcers were induced by oral administration of absolute ethanol or indomethacin 45min after oral pretreatment with EOHc, vehicle and positive control drugs. One hour after the ulcerative challenges, the stomachs were removed and the area of the lesions was measured. The volume, pH and total acidity of the gastric secretions were determined using the pylorus ligature model. The gastrointestinal motility was measured using gastric emptying and intestinal transit. The ethanol-induced gastric mucus depletion and lipid peroxidation were also analyzed. Our findings are as follows: A significant inhibition of gastric lesions induced by absolute ethanol was observed in the mice pre-treated with EOHc, at a dose of 30 and 100 and 300mg/kg (5.56±1.51, 2.88±0.82 and 1.71±0.54mm(2), respectively) compared to control group (118.03±35.4mm(2)). Also, EOHc (300mg/kg) produced a gastroprotective effect against the gastric lesions induced by indomethacin (16.07±4.68mm(2)) compared to control group (38.64±6.1mm(2)). EOHc pretreatment produced a reduction in the ethanol-induced lipid peroxidation from 3.9±0.22 to 2.4±0.1μmol/mg tissue (EOHc-300mg/kg and control group, respectively). We also observed that EOHc pretreatment decreased the gastric emptying, but did

  10. Orofacial antinociceptive effect and antioxidant properties of the hydroethanol extract of Hyptis fruticosa salmz ex Benth.

    Science.gov (United States)

    de Lima, Amanda C B; Paixão, Monica S; Melo, Mônica; de Santana, Marilia T; Damascena, Nicole P; Dias, Antonio S; Porto, Yasmin C B S; Fernandes, Ximene A; Santos, Clisiane C S; Lima, Clésio A; Quintans Júnior, Lucindo J; dos S Estevam, Charles; Araújo, Brancilene S

    2013-03-07

    Hyptis fruticosa is a plant native to Brazil with antinociceptive and antiinflamatory properties. This study evaluated the antinociceptive activity of the hydroethanol extract of the plant leaves (CHEE) against orofacial pain as well as its in vitro effect against lipid peroxidation. The antinociceptive activity was investigated in mice orally treated with different doses of the CHEE (50, 100, and 200 mg/kg) and morphine (5 mg/kg) using formalin, glutamate, and capsaicin orofacial pain models using. Lipoperoxidation was induced in egg yolk by AAPH and FeSO4 in the absence and presence of the CHEE (5, 50, 100, and 150 μg/mL). CHEE (200 mg/kg) significantly reduced (ρHyptis fruticosa leaf CHEE is of pharmacological interest because it was able to inhibit the peripheral and central transmission of orofacial pain, while reducing the spreading of the inflammatory processes by neutralizing reactive oxygen species, which are by-products in the biosynthesis of pain mediators. Copyright © 2013 Elsevier Ireland Ltd. All rights reserved.

  11. Vasorelaxant effect of Hyptis fruticosa Salzm. ex Benth., Lamiaceae, dichloromethane extract on rat mesenteric artery

    Directory of Open Access Journals (Sweden)

    Ítalo J. A. Moreira

    2010-11-01

    Full Text Available Vasorelaxant effect of Hyptis fruticosa dichloromethane extract (HFDE on isolated rings of rat mesenteric artery was evaluated in this study. In intact rings, HFDE (0.1-3000 µg/mL induced concentration-dependent vasorelaxations (Emax = 119±14%; n = 6 of phenylephrine tonus that were not modified after endothelium removal (Emax = 116±6%; n = 6, after KCl 20 mM (Emax = 135±9%; n = 6 or in rings pre-contracted with KCl 80 mM (Emax = 125±4%; n = 6. In endothelium denuded rings, HFDE (300 or 1000 µg/mL inhibited contractions induced by CaCl2 (maximal inhibition = 25±7% and 95±1%; respectively. Furthermore, HFDE promoted an additional vasorelaxation (15±3%; n = 7 after maximal response of 10 µM nifedipine (78±3%; n = 7. In conclusion, HFDE induces vasorelaxant effect through an endothelium-independent pathway, which appears to be due in major part to inhibition of the Ca2+ influx through voltage-operated Ca2+ channels.

  12. Vasorelaxant effect of Hyptis fruticosa Salzm. ex Benth., Lamiaceae, dichloromethane extract on rat mesenteric artery Efeito vasorelaxante do extrato diclorometano de Hyptis fruticosa Salzm. ex Benth., Lamiaceae, em artéria mesentérica de ratos

    Directory of Open Access Journals (Sweden)

    Ítalo J. A. Moreira

    2010-11-01

    Full Text Available Vasorelaxant effect of Hyptis fruticosa dichloromethane extract (HFDE on isolated rings of rat mesenteric artery was evaluated in this study. In intact rings, HFDE (0.1-3000 µg/mL induced concentration-dependent vasorelaxations (Emax = 119±14%; n = 6 of phenylephrine tonus that were not modified after endothelium removal (Emax = 116±6%; n = 6, after KCl 20 mM (Emax = 135±9%; n = 6 or in rings pre-contracted with KCl 80 mM (Emax = 125±4%; n = 6. In endothelium denuded rings, HFDE (300 or 1000 µg/mL inhibited contractions induced by CaCl2 (maximal inhibition = 25±7% and 95±1%; respectively. Furthermore, HFDE promoted an additional vasorelaxation (15±3%; n = 7 after maximal response of 10 µM nifedipine (78±3%; n = 7. In conclusion, HFDE induces vasorelaxant effect through an endothelium-independent pathway, which appears to be due in major part to inhibition of the Ca2+ influx through voltage-operated Ca2+ channels.O efeito vasorelaxante do extrato diclorometano de Hyptis fruticosa Salzm. ex Benth., Lamiaceae (HFDE, em anéis isolados de artéria mesentérica de ratos foi avaliado nesse estudo. Em anéis intactos, pré-contraídos com fenilefrina (10 µM, HFDE (0,1-3000 µg/mL induziu vasorelaxamento de maneira dependente de concentração (Emax = 119±14%; n = 6, o qual não foi afetado após remoção do endotélio (Emax = 116±6%; n = 6, após KCl 20 mM (Emax = 135±9%; n = 6 ou em anéis pré-contraídos com KCl 80 mM (Emax = 125±4%; n = 6. Em anéis sem endotélio, HFDE (300 ou 1000 µg/mL inibiu as contrações induzidas por CaCl2 (inibição máxima = 25±7% e 95±1%, respectivamente. Além disso, HFDE promoveu um vasorelaxamento adicional (15±3%; n = 7 sobre o relaxamento máximo de 10 µM de nifedipina (78±3%, n = 7. Em conclusão, HFDE induz efeito vasorelaxante através de uma via independente de endotélio, possivelmente devido à inibição do influxo de Ca2+ através de canais de Ca2+ operados por voltagem.

  13. Preliminary study on the anti-inflammatory and antioxidant activities of the leave extract of Hyptis fruticosa Salzm. ex Benth., Lamiaceae

    Directory of Open Access Journals (Sweden)

    Alexandre M. Andrade

    2010-10-01

    Full Text Available Various species of Hyptis are used in folk medicine as anti-inflammatory. In order to evaluate the actions of Hyptis fruticosa Salzm. ex Benth., Lamiaceae, studies were performed on anti-inflammatory and antioxidant activities. The ethanol extract (EE of H. fruticosa leaves and its n-CH, CHCl, EtOAc, and MeOH/HO partitions were used in the following experiments. Oral treatment with the EE of H. fruticosa leaves (100, 200, and 400 mg/kg or its n-C6H14, EtOAc, and MeOH/H2O partitions (50, 100, and 200 mg/kg elicited inhibitory activity (p<0.05 on carrageenan-induced oedema formation and leukocyte migration into the peritoneal cavity in rats. However, the CHCl3 partition did not show any inhibitory effect on paw oedema and peritonitis experimental models. The EE and EtOAc partition present highest antioxidant potential (IC50 = 35.00±1.01 and 36.67±2.65 µg/mL DPPH, respectively, similar to the reference compound (IC50 = 16.67±1.21 µg/mL. In conclusion, H. fruticosa shows anti-inflammatory and antioxidant activities.

  14. Avaliação do efeito antinociceptivo e da toxicidade aguda do extrato aquoso da Hyptis fruticosa Salmz. ex Benth

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    Aline B.L. Silva

    Full Text Available Este trabalho descreve o efeito antinociceptivo e a toxicidade aguda do extrato aquoso das folhas da Hyptis fruticosa Salmz. ex Benth. (Lamiaceae. O extrato aquoso liofilizado, administrado por via oral, reduziu as contorções abdominais induzidas por ácido acético (200, 400 e 500 mg/kg e o tempo de reação dos animais na primeira fase do teste da formalina (100 mg/kg e 400 mg/kg. No teste da placa quente, o extrato aquoso aumentou o tempo de latência ao calor (100 e 200 mg/kg tendo este efeito sido revertido pelo antagonista opióide naloxona (5 mg/kg; i.p.. No ensaio de toxicidade aguda, não foi detectada a morte de nenhum animal após tratamento com doses de até 5 g/kg (v.o. do extrato. Em conclusão, os resultados obtidos indicam que o extrato aquoso da Hyptis fruticosa apresenta efeito antinociceptivo em camundongos e não apresenta toxicidade aguda nas doses testadas.

  15. Preliminary study on the anti-inflammatory and antioxidant activities of the leave extract of Hyptis fruticosa Salzm. ex Benth., Lamiaceae Avaliação das atividades anti-inflamatória e antioxidante do extrato etanólico das folhas da Hyptis fruticosa Salzm. ex Benth., Lamiaceae

    Directory of Open Access Journals (Sweden)

    Alexandre M. Andrade

    2010-12-01

    Full Text Available Various species of Hyptis are used in folk medicine as anti-inflammatory. In order to evaluate the actions of Hyptis fruticosa Salzm. ex Benth., Lamiaceae, studies were performed on anti-inflammatory and antioxidant activities. The ethanol extract (EE of H. fruticosa leaves and its n-CH, CHCl, EtOAc, and MeOH/HO partitions were used in the following experiments. Oral treatment with the EE of H. fruticosa leaves (100, 200, and 400 mg/kg or its n-C6H14, EtOAc, and MeOH/H2O partitions (50, 100, and 200 mg/kg elicited inhibitory activity (pVárias espécies do gênero Hyptis são utilizadas na medicina popular para tratar processos inflamatórios. Para avaliar as ações anti-inflamatória e antioxidante da Hyptis fruticosa Salzm. ex Benth., Lamiaceae, utilizou-se extrato etanólico (EE das folhas desta planta e suas partições n-C6H14, CHCl3, AcOEt e MeOH/H2O. O tratamento oral com o EE das folhas da H. fruticosa (100, 200 e 400 mg/kg ou suas partições n-C6H14, AcOEt e MeOH/H2O (50, 100 e 200 mg/kg apresentou atividade inibitória sobre a formação de edema e migração leucocitária para a cavidade peritoneal induzidas pela carragenina em ratos (p<0,05. Entretanto, a partição CHCl3 não causou nenhum efeito sobre a formação de edema e migração de células peritoneais. O EE bruto e a partição AcOEt apresentaram alto potencial antioxidante (IC50 = 35,00±1,01 e 36,67±2,65 µg/mL DPPH, respectivamente, similar ao composto referência (IC50 = 16,67±1,21 µg/mL. Em conclusão, demonstrou-se que a H. fruticosa apresenta atividades anti-inflamatória e antioxidante.

  16. Insecticidal activity of Ageratum conyzoides L., Coleus aromaticus Benth. and Hyptis suaveolens (L.) Poit essential oils as fumigant against storage grain insect Tribolium castaneum Herbst.

    Science.gov (United States)

    Jaya; Singh, Priyanka; Prakash, Bhanu; Dubey, N K

    2014-09-01

    Essential oils (EOs) from Ageratum conyzoides L., Coleus aromaticus Benth. and Hyptis suaveolens (L.) Poit were extracted and tested against Tribolium castaneum Herbst, the storage grain insect. The EOs were found effective against Tribolium castaneum during in vitro as well as in vivo fumigant testing. The EOs of H. suaveolens and A. conyzoides showed 100 % mortality of test insect at 250 ppm while C. aromaticus at 350 ppm. During in vivo fumigant testing of wheat samples against Tribolium castaneum, the essential oils of A. conyzoides and C. aromaticus completely checked the damage of wheat grains by the insect at 1000 ppm while essential oil of H. suaveolens checked the grain damage completely even at 500 ppm concentration. There was no adverse effect on seed germination as well as on seedling growth of EOs treated seeds showing non-phytotoxic nature of the oils. Hence, these EOs may be recommended as botanical insecticide against insect invasion of stored food commodities, thereby enhancing their shelf life.

  17. Morphology and histochemistry of glandular trichomes in Hyptis villosa Pohl ex Benth. (Lamiaceae and differential labeling of cytoskeletal elements

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    Luiz Ricardo dos Santos Tozin

    Full Text Available ABSTRACT Lamiaceae contains many species known for their aromatic properties that are produced by the production of essential oils in glandular trichomes. Hyptis is one of the most common genera of Lamiaceae in the Brazilian flora, and includes several species with medicinal value. However, studies on the morphology and functioning of their glandular trichomes are lacking. We analyzed the morphology, histochemistry and ultrastructure of the glandular trichomes in leaves of H. villosa, emphasizing the differential distribution of actin filaments and microtubules in cells secreting hydrophilic and lipophilic compounds. Four morphotypes of glandular trichomes were identified. Total lipid, terpenes, alkaloids, phenolic compounds, proteins and polysaccharides were histochemically detected in all morphotyes. This evidences the mixed nature of the secretions of this species, although there are differences in the prevalence of lipophilic and hydrophilic components among the glandular morphotypes and among the cells of the same trichome. The actin microfilaments are more abundant in cells that secrete mainly hydrophilic compounds, and microtubules predominate in cells that secrete lipophilic compounds. Our results corroborate the correlation between the glandular morphotype and the composition of the secretion produced, with a differential distribution of the cytoskeletal elements according to the prevalence of either hydrophilic or lipophilic substances.

  18. Antioxidant capacity and biological activity of essential oil and methanol extract of Hyptis crenata Pohl ex Benth

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    Monaliza M. Rebelo

    Full Text Available The essential oils of fresh and dried leaves and fine stems of Hyptis crenata furnished the following yields: 1.4% and 0.9%. The main volatile constituents were ±-pinene (22.0%; 19.5%, 1,8-cineole (17.6%; 23.2%, ²-pinene (17.0%; 13.8%, camphor (4.7%; 11.6%, limonene (5.4%; 4.4% and ³-terpinene (3.5%; 2.4%, totalizing more than 70% in the oils. The DPPH radical scavenging activity (EC50, 16.7 + 0.4 µg/mL of the methanol extract was comparable to BHT (19.8 ± 0.5 µg/mL showing a significant antioxidant activity. The oils showed low activities. The amount of total phenolics (TP, 373.0 + 15.9 mg GAE/g and trolox equivalent (TEAC, 226.8 + 0.5 mg TE/g confirmed the antioxidant activity of the methanol extract that can be attributed to the presence of polar phenolic compounds. In the brine shrimp bioassay the lethal concentrations (LC50 for the oil and methanol extract were 6.7 + 0.2 µg/mL and 13.0 + 3.7 µg/mL, respectively, providing important evidence of their biological activities.

  19. Essential oils from the Hyptis genus--a review (1909-2009).

    Science.gov (United States)

    McNeil, Megil; Facey, Petrea; Porter, Roy

    2011-11-01

    Several species belonging to the Hyptis (Lamiaceae) genus represent an important source of bioactive constituents, which are reputed for their wide range of antimicrobial, anticancer and insecticidal activities. The volatile oils obtained from various parts of the Hyptis plants were found to be primarily composed of mono- and sesquiterpenes. Significant differences were observed in the percentage compositions of the major components, which allowed for differentiation among the species. Based on the dominant constituents, phylogenetic relationships were found to be common among some species: 1,8-cineole (H. fruticosa, H. goyazensis, H. martiusii and H. suaveolens); beta-caryophyllene (H. marrubioides, H. pectinata, H. spicigera and H. suaveolens); eugenol (H. recurvata and H. suaveolens); gamma-cadinene (H. glomerata and H. ovalifolia); p-cymene (H. mutabilis and H. pectinata); alpha-pinene (H. crenata and H. emoryi). The monoterpenes, alpha-pinene and p-cymene were detected at various concentrations in all the Hyptis oils investigated. This paper reviews the essential oil compositions of eighteen Hyptis plant species studied in various parts of the world covering the publications of 100 years (1909 to 2009) after the first article appeared in the literature.

  20. Essential oil composition and variability in Hyptis fruticosa

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    Clóvis R. P. Franco

    2011-02-01

    Full Text Available The composition of six samples of essential oil (EO extracted from leaves, flowers and seeds of several plants of Hyptis fruticosa Salzm. ex Benth., Lamiaceae, was investigated by GC/MS and GC/FID. 1,8-Cineole, spathulenol, α-pinene, β-pinene were the major constituents. Ten constituents that have not been previously described in the composition of the oil of H. fruticosa were identified. Hydrocarbons sesquiterpenes represented the main group, followed by hydrocarbons monoterpenes. The results were submitted to Cluster Analysis which allowed three groups of EO to be distinguished with respect to the content of α-pinene/β-pinene, 1,8-cineole and spathulenol. Growth stages of the plants and geographical parameters seem to be important factors determining the variability of the oil. Sesquiterpenes were mainly produced in the seeds.

  1. Essential oil composition and variability in Hyptis fruticosa

    Directory of Open Access Journals (Sweden)

    Clóvis R. P. Franco

    2011-03-01

    Full Text Available The composition of six samples of essential oil (EO extracted from leaves, flowers and seeds of several plants of Hyptis fruticosa Salzm. ex Benth., Lamiaceae, was investigated by GC/MS and GC/FID. 1,8-Cineole, spathulenol, α-pinene, β-pinene were the major constituents. Ten constituents that have not been previously described in the composition of the oil of H. fruticosa were identified. Hydrocarbons sesquiterpenes represented the main group, followed by hydrocarbons monoterpenes. The results were submitted to Cluster Analysis which allowed three groups of EO to be distinguished with respect to the content of α-pinene/β-pinene, 1,8-cineole and spathulenol. Growth stages of the plants and geographical parameters seem to be important factors determining the variability of the oil. Sesquiterpenes were mainly produced in the seeds.

  2. Effet de L'hyptis ( Hyptis suaveolens ), du Neem ( Azadirachta indica ...

    African Journals Online (AJOL)

    Effet de L'hyptis ( Hyptis suaveolens ), du Neem ( Azadirachta indica ), du Vernonia ( Vernonia amygdalina ), et de L'amarante ( Amaranthus sp.) sur les nematodes A Galles ( Meloidogyne spp.) en cultures Maraicheres.

  3. Abietane diterpenes from Hyptis suaveolens.

    Science.gov (United States)

    Prawatsri, Saowanee; Suksamrarn, Apichart; Chindaduang, Anon; Rukachaisirikul, Thitima

    2013-08-01

    Investigation of the constituents of the whole plant of Hyptis suaveolens led to the isolation of three new abietane diterpenes, isosuaveolic acid (1), 8α,9α-epoxysuaveolic acid (2), and 14-O-methylsuaveolic acid (3), together with eleven known compounds. The structures of 1-3 were established by spectroscopic methods and chemical correlations. Some isolates were tested for their antimycobacterial and cytotoxic activities. Copyright © 2013 Verlag Helvetica Chimica Acta AG, Zürich.

  4. Abietane diterpenes from Hyptis platanifolia

    OpenAIRE

    Araújo,Edigênia C. C.; Lima,Mary Anne S.; Nunes,Edson P.; Silveira,Edilberto R.

    2005-01-01

    Three new abietane diterpenes 19-oxo-inuroyleanol, 11,14-dihydroxy-12-methoxy-7-oxo-8,11,13-abietatrien-19,20 beta-olide and 19,20-epoxy-12-methoxy-11,14,19-trihydroxy-7-oxo-8,11,13 -abietatriene, in addition to inuroyleanol, and coulterone were isolated from roots of Hyptis platanifolia. Investigation of the aerial parts yielded a mixture of the steroids stigmasterol and beta-sitosterol, and the triterpenes betulinic and ursolic acids. Structural characterization of all compounds was establi...

  5. Abietane diterpenes from Hyptis platanifolia

    Energy Technology Data Exchange (ETDEWEB)

    Araujo, Edigenia C.C.; Lima, Mary Anne S.; Silveira, Edilberto R. [Ceara Univ., Fortaleza, CE (Brazil). Dept. de Quimica Organica e Inorganica. Curso de Pos-Graduacao em Quimica Organica]. E-mail: edil@ufc.br; Nunes, Edson P. [Ceara Univ., Fortaleza, CE (Brazil). Dept. de Biologia

    2005-11-15

    Three new abietane diterpenes 19-oxo-inuroyleanol, 11,14-dihydroxy-12-methoxy-7-oxo-8,11,13-abietatriene-19,20 {beta}-olide and 19,20-epoxy-12-methoxy-11,14,19-trihydroxy-7-oxo-8,11,13 -abietatriene, in addition to inuroyleanol, and coulterone were isolated from roots of Hyptis platanifolia. Investigation of the aerial parts yielded a mixture of the steroids stigmasterol and {beta}-sitosterol, and the triterpenes betulinic and ursolic acids. Structural characteriza of all compounds was established on the basis of spectroscopic methods, particularly 1D and 2D NMR, and comparison with data from literature. (author)

  6. Hyptis uliginosa (lamiaceae en la Argentina

    Directory of Open Access Journals (Sweden)

    Sara G. Tressens

    2003-01-01

    Full Text Available Se cita por primera vez para la Argentina a Hyptis uliginosa, perteneciente a la sección Plagiotis. Ninguna de las tres especies de esta sección había sido mencionada aún para nuestro país. Se describe e ilustra la nueva cita y se incluye una clave para identificar las especies de Hyptis de Argentina

  7. Hyptis uliginosa (lamiaceae) en la Argentina

    OpenAIRE

    Sara G. Tressens; Keller, Héctor A.

    2003-01-01

    Se cita por primera vez para la Argentina a Hyptis uliginosa, perteneciente a la sección Plagiotis. Ninguna de las tres especies de esta sección había sido mencionada aún para nuestro país. Se describe e ilustra la nueva cita y se incluye una clave para identificar las especies de Hyptis de Argentina

  8. Abietane diterpeniods from Hyptis verticillata.

    Science.gov (United States)

    Porter, Roy B R; Biggs, Duanne A C; Reynolds, William F

    2009-01-01

    Seven abietane diterpenoids were isolated from the roots of Hyptis verticillata (Labiatae). One is a novel natural product, 7-acetoxy-16-benzoxy-12-hydroxyabieta-8,12-diene-11,14-dione (1), while the other six known compounds are 11,14 dihydroxy-12-methoxyabieta-8,11,13-triene-7-one (2), 11,14-dihydroxy-12-methoxy- 18(4-->3betaH) abeo-abieta-4(19),8,11,13-tetraene-7-one (3), 7-acetoxy-12-methoxyabieta-8,12-diene-11,14-dione (4), royleanone (5), 7,6-dehydroroyleanone (6) and 7-acetoxyhorminone (7). All structures were elucidated by spectroscopic and mass spectrometric means.

  9. Estudo farmacobotânico comparativo das folhas de Hyptis pectinata (L.) Poit. e Hyptis suaveolens (L.) Poit. (Lamiaceae)

    OpenAIRE

    Lima Diniz Basílio, Ionaldo José; Agra, Maria de Fátima; Rocha, Emerson Antônio; Abreu Leal, Crislaine Kieva; Figueiredo Abrantes, Hálamo

    2006-01-01

    Realizou-se um estudo farmacobotânico comparativo entre Hyptis pectinata (L.) Poit. e Hyptis suaveolens (L.) Poit. O gênero Hyptis Jacq. (Lamiaceae) possui cerca de 300 espécies, com ampla distribuição, ocorrendo principalmente nas regiões tropicais das Américas e África. No Nordeste do Brasil, Hyptis pectinata e Hyptis suaveolens são empregadas na medicina popular e comercializadas pelos raizeiros e vendedores de plantas medicinais nas feiras livres da região. As análises macroscópicas e mic...

  10. Antinociceptive effect of Hyptis pectinata leaves extracts.

    Science.gov (United States)

    Lisboa, Ana C C D; Mello, Iderjane C M; Nunes, Rogeria S; Dos Santos, Marquês A; Antoniolli, Angelo R; Marçal, Rosilene M; Cavalcanti, Sócrates C de H

    2006-09-01

    Oral administration of hexanes, chloroform, and ethyl acetate extracts of the leaves of Hyptis pectinata significantly reduced the number of writhing induced by acetic acid and increased the response to thermal stimuli in hot-plate test. Such effect was completely reversed by the opioid antagonist naloxone.

  11. New labdane diterpenoids from Hyptis fasciculata.

    Science.gov (United States)

    Ohsaki, Ayumi; Kishimoto, Yoshiko; Isobe, Takahiko; Fukuyama, Yoshiyasu

    2005-12-01

    Two new labdane diterpenoids, 15beta-methoxyfaciculatin (1) and 15alpha-methoxyfaciculatin B (2), together with the previously known methoxynepetaefolin (3), were isolated from a methanol extract of the dried aerial parts of a Brazilian medicinal plant, Hyptis faciculata. Their structures were elucidated by analysis of spectroscopic data. Plausible biogenetic correlation between faciculatins and nepetaefolin is briefly discussed.

  12. Chemical composition of Hyptis suaveolens and Ocimum ...

    African Journals Online (AJOL)

    Four medicinal plants belonging to the family Lamiaceae were chemically screened for their chemical constituents including alkaloids, tannins, saponins, flavonoids and phenols. The medicinal plants investigated were Hyptis suaveloens and three putative hybrids of Ocimum gratissimum (Hybrid A, B and C). All the plants ...

  13. Phytochemical evaluation and GC-MS analysis of Hyptis verticillata ...

    African Journals Online (AJOL)

    parts of America and the Caribbean countries. (Picking et al., 2013). Hyptis verticillata has been used medicinally in Mesoamerica since the times of the Aztec and Mayan cultures. (Ocampo and Balick, 2009). It is among the most abundant species of the genus Hyptis and it grows up to the height of one to two meters.

  14. New humulenes from Hyptis incana (Labiatae).

    Science.gov (United States)

    Satoh, Mitsuru; Satoh, Yoshio; Anzai, Yasuhiro; Ajisawa, Daisuke; Matsuzaki, Keiichi; Makino, Mitsuko; Fujimotod, Yasuo

    2013-12-01

    Two new humulene-type sesquiterpenes, named hyptishumulene I (1) and II (2), have been isolated, together with eight known compounds, a humulene-type sesquiterpene (3), a monoterpene (4) and six abietane-type diterpenoids (5-10) from the aerial parts of Hyptis incana (Labiatae). The cytotoxic activity of the isolated compounds against mouse leukemia cells (L1210) was examined. The abietane-type diterpenoids (5-10) showed rather potent growth inhibitory activity (IC5050 microM).

  15. Antimicrobial activity of Hyptis ovalifolia towards dermatophytes.

    Science.gov (United States)

    Souza, Lúcia K H; de Oliveira, Cecília M A; Ferri, Pedro H; de Oliveira Júnior, Juldásio Galdino; de Souza Júnior, Ary Henrique; Fernandes, Orionalda de Fátima Lisboa; Silva, Maria do Rosário R

    2003-10-01

    The essential oil and the aqueous, hexane and methanolic fractions from Hyptis ovalifolia leaves were evaluated for their antifungal activity in vitro against 60 strains of dermatophytes: 10 strains of Microsporum canis, 10 of M. gypseum, 20 of Trichophyton rubrum and 20 of T. mentagrophytes. The extracts inhibited growth of the dermatophytes tested at different concentrations. The most biologically active was the essential oil from the leaves which inhibited 57 isolates (95%) at a concentration of 500 g/ml.

  16. Antimicrobial activity of Hyptis ovalifolia towards dermatophytes

    Directory of Open Access Journals (Sweden)

    Lúcia KH Souza

    2003-10-01

    Full Text Available The essential oil and the aqueous, hexane and methanolic fractions from Hyptis ovalifolia leaves were evaluated for their antifungal activity in vitro against 60 strains of dermatophytes: 10 strains of Microsporum canis, 10 of M. gypseum, 20 of Trichophyton rubrum and 20 of T. mentagrophytes. The extracts inhibited growth of the dermatophytes tested at different concentrations. The most biologically active was the essential oil from the leaves which inhibited 57 isolates (95% at a concentration of £ 500 µg/ml.

  17. Comparative toxicity effect of bush tea leaves ( Hyptis suaveolens ...

    African Journals Online (AJOL)

    Hyptis suaveolens) were compared for their toxicity effect on the larvae of the yellow fever mosquito Aedes aegypti collected from disused tyres beside College of Natural Sciences building University of Agriculture, Abeokuta, Nigeria.

  18. Una nueva especie de Hyptis (Labiatae de Colombia

    Directory of Open Access Journals (Sweden)

    Fernández-Alonso, José Luis

    2010-12-01

    Full Text Available The shruby new species, Hyptis jacobi Fern. Alonso, only known from the central Chicamocha valley (Department of Santander in the western slope of the Cordillera Oriental of Colombia, is described and illustrated. This species is related to the Andine H. tafallae (Sect. Umbellatae but is quite different from other Hyptis previously known from Colombia.Se describe e ilustra Hyptis jacobi Fern. Alonso, una nueva especie de porte arbustivo, conocida sólo de la cuenca media del río Chicamocha (departamento de Santander en la vertiente occidental de la Cordillera Oriental de Colombia. Esta planta que tiene alguna afinidad con la especie andina H. tafallae (sección Umbellatae es muy diferente de las especies de Hyptis conocidas previamente de Colombia.

  19. Phytochemical Screening and Antidiarrhoeal Activity of Hyptis suaveolens

    OpenAIRE

    Md. Zeshan Hashib Shaikat; Md.Taleb Hossain; Md. Golam Azam

    2012-01-01

    Summary. Hyptis suaveolens leaf has been used in conventional therapies for various disease conditions, including diarrhea. However, some of the therapeutic potentials of the plant have not been scientifically evaluated. Hence, the present study was aimed to evaluate the antidiarrhoeal activity of ethanol extract of Hyptis suaveolens leaf against an experimental model of castor oil-induced diarrhea in mice. Phytochemical screening of the plant extracts for their active constituents was also p...

  20. Ionic Liquid-Based Microwave-Assisted Extraction of Flavonoids from Bauhinia championii (Benth. Benth.

    Directory of Open Access Journals (Sweden)

    Wei Xu

    2012-12-01

    Full Text Available An ionic liquids (IL-based microwave-assisted approach for extraction and determination of flavonoids from Bauhinia championii (Benth. Benth. was proposed for the first time. Several ILs with different cations and anions and the microwave-assisted extraction (MAE conditions, including sample particle size, extraction time and liquid-solid ratio, were investigated. Two M 1-butyl-3-methylimidazolium bromide ([bmim] Br solution with 0.80 M HCl was selected as the optimal solvent. Meanwhile the optimized conditions a ratio of liquid to material of 30:1, and the extraction for 10 min at 70 °C. Compared with conventional heat-reflux extraction (CHRE and the regular MAE, IL-MAE exhibited a higher extraction yield and shorter extraction time (from 1.5 h to 10 min. The optimized extraction samples were analysed by LC-MS/MS. IL extracts of Bauhinia championii (Benth.Benth consisted mainly of flavonoids, among which myricetin, quercetin and kaempferol, β-sitosterol, triacontane and hexacontane were identified. The study indicated that IL-MAE was an efficient and rapid method with simple sample preparation. LC-MS/MS was also used to determine the chemical composition of the ethyl acetate/MAE extract of Bauhinia championii (Benth. Benth, and it maybe become a rapid method to determine the composition of new plant extracts.

  1. Chromosomal aberrations induced by Markhamia tomentosa (Benth ...

    African Journals Online (AJOL)

    Markhamia tomentosa (Benth.) K. Schum. Ex Engl. (Bignoniaceae) is used traditionally in the treatment of pain, oedema, pulmonary troubles and cancer. The genotoxic and cytotoxic effects of the ethanolic extract of the leaves of M. tomentosa was investigated using the Allium cepa root chromosomal aberration assay.

  2. Chemical composition and seasonal variability of the essential oils of leaves and morphological analysis of Hyptis carpinifolia

    Directory of Open Access Journals (Sweden)

    Stone de Sá

    Full Text Available ABSTRACT Hyptis carpinifolia Benth., Lamiaceae, is a species known popularly as "rosmaninho" and "mata-pasto", and leaves are employed in Brazilian folk medicine to treat colds, flu, and rheumatism. The aim of this study was to perform a morphological description of H. carpinifolia and to evaluate the seasonal chemical variability of the leaf essential oils during 12 months. Macroscopic characterization of H. carpinifolia was carried out with the naked eye and with a stereoscopic microscope. Essential oils were isolated from leaves by hydrodistillation in Clevenger apparatus and analyzed by gas chromatography/mass spectrometry. Major compounds were found to be 1,8-cineole (39.6-61.8%, trans-cadina-1(6,4-diene (2.8-17.5%, β-caryophyllene (4.4-10.0%, prenopsan-8-ol (4.2-9.6% and β-pinene (2.9-5.3%. Results of essential oils compositions were processed by cluster analysis and principal component analysis. Data showed high variability in the concentration of the components. Besides, there was a seasonal variability of chemical composition, probably related mainly to the rainfall regime.

  3. Potentiel antimicrobien des extraits de feuilles d' Hyptis suaveolens ...

    African Journals Online (AJOL)

    The antimicrobial activity of Hyptis suaveolens leaves extracts was evaluated on some microorganisms to justify the traditional use of the plant. Successive extractions were performed on 100 g leaves powder by a solvent system increasing polarity. The best performance was obtained with aqueous extraction : 4.43% dry ...

  4. Antiplasmodial diterpenoid from the leaves of Hyptis suaveolens.

    Science.gov (United States)

    Chukwujekwu, J C; Smith, P; Coombes, P H; Mulholland, D A; van Staden, J

    2005-11-14

    Bioactivity-guided fractionation of the petroleum ether extract of the leaves of Hyptis suaveolens, widely used in Traditional Medicine, has led to the isolation of an abietane-type diterpenoid endoperoxide, 13 alpha-epi-dioxiabiet-8(14)-en-18-ol, displaying high antiplasmodial activity (IC(50) 0.1 microg/ml).

  5. The molluscicidal effects of Hyptis suaveolens on different stages of ...

    African Journals Online (AJOL)

    The molluscicidal effect of the ethanolic extract of the plant Hyptis suaveolens was evaluated against different stages: eggs, juveniles and adults of the freshwater snail Bulinus globosus. Ten healthy one week old juveniles were exposed to different concentrations of the extract for 3 h, while three to four weeks old snails and ...

  6. INHIBITION OF CALCIUM OXALATE CRYSTALLIZATION IN-VITRO BY VARIOUS EXTRACTS OF HYPTIS SUAVEOLENS (L.) POIT.

    OpenAIRE

    Agarwal Kumkum; Varma Ranjana

    2012-01-01

    Hyptis suaveolens (L) Poit. commonly known as Vilayati tulsi, belongs to the Mint family Lamiaceae. The inhibition of in-vitro calcium-oxalate crystal (a major component of most urinary stones) formation by various extracts of Hyptis was investigated by titrimetric method. The inhibitor potency of alcohol extracts of Hyptis suaveolens (L.) Poit was found to be comparable to that of cystone (a proprietary drug for dissolving kidney stones). Thus alcohol extract could be further analyzed in viv...

  7. Hyptis pectinata: redox protection and orofacial antinociception.

    Science.gov (United States)

    Paixão, M S; Melo, M S; Oliveira, M G B; Santana, M T; Lima, A C B; Damascena, N P; Dias, A S; Araujo, B S; Estevam, C S; Botelho, M A; Quintans, L J

    2013-09-01

    Hyptis pectinata L. Poit, known as 'sambacaitá', is used in Brazil to treat inflammatory and painful disorders. In this study, the antioxidant and orofacial antinociceptive properties of the aqueous extract of H. pectinata leaves (AEPH) were assessed using in vitro and in vivo models. Thus, AEPH reduced the 2,2-diphenyl-1-picrylhydrazyl radical up to 72.10% with an EC₅₀ of 14.56 µg/ml. It also inhibited 40.80% of the lipoperoxidation induced by 2'-azobis (2-amidinopropane) dihydrochloride in the thiobarbituric acid-reactive substances assay. The orofacial antinociceptive activity was evaluated in mice pre-treated with AEPH (100, 200 and 400 mg/kg, p.o.) and morphine (5 mg/kg, i.p.), which received afterwards formalin- (20 µl, 2% solution, s.c.), glutamate- (40 µl, 25 mM, s.c.) and capsaicin- (20 µl, 2.5 µg, s.c.) to induce orofacial nociception. AEPH at all doses reduced (p < 0.001) the nociceptive response in the first (43-62%) and second (47-80%) phases of the formalin test. Besides, the effect of AEPH (400 mg/kg) was not changed in the presence of naloxone (1.5 mg/kg, i.p.), an opioid antagonist. AEPH significantly inhibited mice face rubbing for capsaicin (23-69%, p < 0.05) and glutamate (48-77%, p < 0.001) at all doses. The findings suggested the AEPH has peripheral and central antinociceptive activities, which are not related to opioid receptors. Copyright © 2012 John Wiley & Sons, Ltd.

  8. Variedades químicas de Hyptis mutabilis

    OpenAIRE

    Luz,Α. I. Reis; Zoghbi,Μ. G. Bichara; Silva,M. L. da; L. S. Ramos; Soares Maia,J. G.

    1989-01-01

    RESUMODuas variedades químicas de Hyptis mutabilis foram estudadas. Uma delas, a variedade A, contém α e β-pineno, α e β-feladreno e β-cariofileno como os principais constituintes de seu óleo essencial. A outra variedade B, apresentou α-cimeno , 1,8-cineol, timol, β-cariofileno e β-elemeno como os seus principais componentes.

  9. Sesquiterpene and acyclic diterpenes from Hyptis verticillata Jacq.

    Science.gov (United States)

    Biggs, Duanne A C; Porter, Roy B R; Reynolds, William F

    2009-03-01

    From the methanolic extract of the plant Hyptis verticillata Jacq, was isolated five compounds: the novel compounds cadina-10(15)-en-3-one and 3,7,11,15-tetramethylhexadec-1-en-3-ol and the known compounds 3,7,11,15-tetramethylhexadec-2-en-1-ol, 7,11,15-trimethyl-3-methylenehexadecane-1,2-diol and myo-inositol. All structures were established by spectroscopic means.

  10. Antioxidant and Antimicrobial Activities of Hyptis suaveolens Essential Oil

    OpenAIRE

    Nantitanon, Witayapan; Chowwanapoonpohn, Sombat; Okonogi, Siriporn

    2007-01-01

    The essential oil of Hyptis suaveolens obtained by steam distillation was examined for its antioxidant and antimicrobial activities. The antioxidant activity was determined by means of the DPPH radical scavenging test and ABTS free radical decolorization assay. Results from both methods indicate that the antioxidant activity of H. suaveolens oil is time and concentration dependent. The antioxidant potential of H. suaveolens oil determined by the DPPH method expressed as IC50 was 3.72 mg/ml wh...

  11. Domestication du gros baume ( Hyptis suaveolens (L.) Poit ...

    African Journals Online (AJOL)

    Hyptis suaveolens Poit. est une herbe vivace de la famille des Lamiaceae qui pousse en colonies denses en région tropicale. Elle est originaire de l'Amérique tropicale mais se retrouve aujourd'hui sous forme de mauvaise herbe un peu partout dans le monde. C'est une plante qui dégage une odeur caractéristique ...

  12. Mass propagation of Rauwolfia serpentina L. Benth.

    Science.gov (United States)

    Salma, U; Rahman, M S M; Islam, S; Haque, N; Khatun, M; Jubair, T A; Paul, B C

    2008-05-01

    A protocol for mass propagation through axillary bud proliferation was established for Rauwolfia serpentina L. Benth. (Apocynaceae). MS medium supplemented with 1.5 mg L(-1) BA and 0.2 mg L(-1) NAA elicited the maximum number of shoots (4 multiple shoots) from nodal explants. These adventitious shoots were best rooted on half strength MS medium supplemented with 1.0 mg L(-1) each of IBA and IAA. The in vitro raised plants were acclimatized in glass house and successfully transplanted to field condition with almost 95% survival.

  13. The essential oils chemical compositions and antimicrobial, antioxidant activities and toxicity of three Hyptis species.

    Science.gov (United States)

    Xu, Dian-Hong; Huang, Ya-Si; Jiang, Dong-Qing; Yuan, Ke

    2013-09-01

    Hyptis suaveolens (Linn.) Poit., Hyptis rhomboidea Mart. et Gal., and Hyptis brevipes Poit., are three species of Hyptis Jacq. (Lamiaceae). Hyptis suaveolens is used for the treatment of fever, headache, gastrointestinal bloating and rheumatism in the traditional folk medicine; Hyptis rhomboidea for hepatitis, ulcer and swollen poison; and Hyptis brevipes for asthma and malaria. To characterize chemical compositions of the oils from three Hyptis species and evaluate their potential antimicrobial, radical scavenging activities and toxicities against brine shrimp. The oils were obtained by hydrodistillation, and their chemical compositions were investigated by gas chromatography-mass spectrometry (GC-MS). Minimum inhibitory concentrations (MICs) were determined using the tube double-dilution technique. The antioxidant activities were investigated using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay and toxicities by the brine shrimp bioassay. Forty-seven, 33 and 28 constituents of oils isolated, respectively, from H. suaveolens, H. rhomboidea and H. brevipes were identified. Among the essential oils, the strongest antioxidant activity was exhibited by H. brevipes with an SC₅₀ value of 2.019 ± 0.25 μg mL⁻¹. The H. brevipes oil exhibited the strongest antimicrobial activity (3.125-6.25 μg mL⁻¹) on pathogens employed in the assay. They all showed significant toxicities with median lethal concentration (LC₅₀) values of 62.2 ± 3.07 μg mL⁻¹, 65.9 ± 6.55 μg mL⁻¹ and 60.8 ± 9.04 μg mL⁻¹, respectively. The three Hyptis species oils possess strong antimicrobial activities and toxicities. Hyptis rhomboidea and H. brevipes showed considerable antioxidant activity compared to the positive control.

  14. EFFECT OF PICRORHIZA KURROA BENTH. IN ACUTE INFLAMMATION

    OpenAIRE

    Kantibiswas, Tuhin; Marjit, Bani; Maity, Lakshmi Narayan

    1996-01-01

    The effect of the indigenous drug Picrorhiza kurrooa Benth was studied on experimental acute inflammation in rats. It was observed that Picrorhiza kurrooa at a dose of 100 mg/kg b.w. has significant (p

  15. PHARMACOGNOSTIC STUDY OF LEAF OF HYPTIS SUAVEOLENS L

    Science.gov (United States)

    Jelani, S.; Prabhakar, M.

    1991-01-01

    This paper deals with the pharmacognosy of Hyptis suaveolens leaf including its morphological, anatomical, chemical constituents and powder analysis. Contrary to earlier reports, the leaves are amphistomatic. Stomata are diacytic, a few being isotricytic and ansiotricytic. Four types of trichomes have been noted. The venation is semicraspidodromous. Midrib consists of one large moon shaped and petiole with four wedge-shaped vascular bundles. Powder microscopy revealed fragments of epidermis, mesophyll, trichomes and tracheary elements. Positive tests for glycosides, polyphenolases, flavones and juglones are also recorded. PMID:22556556

  16. Chemical variability in the essential oil of Hyptis suaveolens.

    Science.gov (United States)

    Azevedo, N R; Campos, I F; Ferreira, H D; Portes, T A; Santos, S C; Seraphin, J C; Paula, J R; Ferri, P H

    2001-07-01

    The essential oils of Hyptis suaveolens plants collected from 11 localities of the Brazilian Cerrado region were investigated by GC-MS. Sabinene, limonene, biclyclogermacrene, beta-phellandrene and 1,8-cineole were the principal constituents. The results were submitted to principal component and chemometric cluster analysis which allowed three groups of essential oils to be distinguished with respect to the content of p-mentha-2,4(8)-diene, limonene/beta-phellandrene/gamma-terpinene and germacrene D/bicyclogermacrene. In patterns of geographic variation in essential oil composition indicated that the sesquiterpenes are mainly produced in the samples grown at lower latitudes.

  17. Antileishmanial Phenylpropanoids from the Leaves of Hyptis pectinata (L. Poit

    Directory of Open Access Journals (Sweden)

    Rosangela A. Falcao

    2013-01-01

    Full Text Available Hyptis pectinata, popularly known in Brazil as “sambacaitá” or “canudinho,” is an aromatic shrub largely grown in the northeast of Brazil. The leaves and bark are used in an infusion for the treatment of throat and skin inflammations, bacterial infections, pain, and cancer. Analogues of rosmarinic acid and flavonoids were obtained from the leaves of Hyptis pectinata and consisted of two new compounds, sambacaitaric acid (1 and 3-O-methyl-sambacaitaric acid (2, and nine known compounds, rosmarinic acid (3, 3-O-methyl-rosmarinic acid (4, ethyl caffeate (5, nepetoidin A (6, nepetoidin B (7, cirsiliol (8, circimaritin (9, 7-O-methylluteolin (10, and genkwanin (11. The structures of these compounds were determined by spectroscopic methods. Compounds 1–5, and 7 were evaluated in vitro against the promastigote form of L. braziliensis, and the ethanol extract. The hexane, ethyl acetate, and methanol-water fractions were also evaluated. The EtOH extract, the hexane extract, EtOAc, MeOH:H2O fractions; and compounds 1, 2 and 4 exhibited antileishmanial activity, and compound 1 was as potent as pentamidine. In contrast, compounds 3, 5, and 7 did not present activity against the promastigote form of L. braziliensis below 100 µM. To our knowledge, compounds 1 and 2 are being described for the first time.

  18. Antileishmanial Phenylpropanoids from the Leaves of Hyptis pectinata (L.) Poit.

    Science.gov (United States)

    Falcao, Rosangela A; do Nascimento, Patricia L A; de Souza, Silvana A; da Silva, Telma M G; de Queiroz, Aline C; da Matta, Carolina B B; Moreira, Magna S A; Camara, Celso A; Silva, Tania M S

    2013-01-01

    Hyptis pectinata, popularly known in Brazil as "sambacaitá" or "canudinho," is an aromatic shrub largely grown in the northeast of Brazil. The leaves and bark are used in an infusion for the treatment of throat and skin inflammations, bacterial infections, pain, and cancer. Analogues of rosmarinic acid and flavonoids were obtained from the leaves of Hyptis pectinata and consisted of two new compounds, sambacaitaric acid (1) and 3-O-methyl-sambacaitaric acid (2), and nine known compounds, rosmarinic acid (3), 3-O-methyl-rosmarinic acid (4), ethyl caffeate (5), nepetoidin A (6), nepetoidin B (7), cirsiliol (8), circimaritin (9), 7-O-methylluteolin (10), and genkwanin (11). The structures of these compounds were determined by spectroscopic methods. Compounds 1-5, and 7 were evaluated in vitro against the promastigote form of L. braziliensis, and the ethanol extract. The hexane, ethyl acetate, and methanol-water fractions were also evaluated. The EtOH extract, the hexane extract, EtOAc, MeOH:H2O fractions; and compounds 1, 2 and 4 exhibited antileishmanial activity, and compound 1 was as potent as pentamidine. In contrast, compounds 3, 5, and 7 did not present activity against the promastigote form of L. braziliensis below 100 µM. To our knowledge, compounds 1 and 2 are being described for the first time.

  19. Senyawa Hiptolida Dan Pektinolida Dalam Fraksi Diklorometana Dari Daun Hyptis Pectinata Poit

    OpenAIRE

    Suzery, Meiny; Gultom, Merry; Cahyono, Bambang

    2013-01-01

    Hyptis pectinata Poit merupakan salah satu tanaman Lamiaceae, tanaman ini tumbuh subur dan mudah diperoleh pada tempat yang cukup sinar matahari. Penelitian ini bertujuan untuk memperoleh senyawa bioaktif yang terdapat dalam fraksi diklorometan dari daun Hyptis pectinata Poit. Metode pemisahan menggunakan teknik kromatografi dan penetuan struktur menggunakan spektroskopi inframerah dan GC/MS. Hasil pemisahan dengan kolom kromatografi diperoleh kristal dengan titik leleh titik leleh 88,5-890C,...

  20. Chemical Composition and Antimicrobial Activity of a New Chemotype of Hyptis suaveolens (Poit) from Nigeria

    OpenAIRE

    B.A. Iwalokun; A. Oluwadun; A. Otunba; O.A. Oyenuga

    2012-01-01

    Hyptis suaveolens is one of the aromatic plants credited for substantial medicinal values in the tropics with three chemotypes previously reported in Nigeria. This study provided biological and chemical evidence for a new chemotype of Hyptis suaveolens in Lagos. Hydrodistillation of the dried leaves of the plant produced volatile oil with a yield of 0.31% and subsequent analyses by GC-MS identified 28 volatile compounds that accounted for 99.1% of the total oil composition. Although the oil w...

  1. Occurrence and structure of extrafloral nectariesin Pterodon pubescens Benth. and Pterodon polygalaeflorus Benth.

    Directory of Open Access Journals (Sweden)

    Paiva Élder Antônio Sousa e

    2001-01-01

    Full Text Available Extrafloral nectaries (EFNs are structurally variable and widely spread among the angiosperms. The occurrence of EFNs in leaves of Pterodon polygalaeflorus Benth. and Pterodon pubescens Benth. (Fabaceae: Papilionoideae were detected in adult specimens, at the time of production of new buds and flowers. The goals of the present study are to register the occurrence of the EFNs in P. pubescens and P. polygalaeflorus, and provide comparative data on the anatomical structures. The EFNs occur in the rachis and are located under the insertion of each petiolule. Each nectary consists of a small elevation whose apical portion is deeply invaginated, resulting in a depression (secretory pole, a common characteristic of both species. Unicellular, nonglandular trichomes occur along the rachis, being less numerous in P. polygalaeflorus while in P. pubescens they cover the EFNs. The secretory tissue consists of parenchyma cells with dense cytoplasm compactly arranged. The nectar reaches the surface of the EFNs by rupturing the thin cuticle which covers the secretory pole, since both species lack stomata or any other interruption at the epidermis. The basic difference between the two species, in relation to the EFNs, is the density of the pubescence, which is always greater in P. pubescens. Structural and dimensional modifications may be observed, even between basal and apical nectaries in the same rachis, so it does not constitute a taxonomical tool.

  2. Hedgehog inhibitors from Artocarpus communis and Hyptis suaveolens.

    Science.gov (United States)

    Arai, Midori A; Uchida, Kyoko; Sadhu, Samir K; Ahmed, Firoj; Koyano, Takashi; Kowithayakorn, Thaworn; Ishibashi, Masami

    2015-08-01

    The hedgehog (Hh) signaling pathway plays crucial roles in cell maintenance and proliferation during embryonic development. Naturally occurring Hh inhibitors were isolated from Artocarpus communis and Hyptis suaveolens using our previously constructed cell-based assay system. Bioactivity guided fractionation led to the isolation of 15 compounds, including seven new compounds (4, 5, 6, 7, and 9-11). The isolated compounds showed cytotoxicity against a cancer cell line (PANC1) in which Hh signaling was abnormally activated. Several compounds (12-14; GLI1 transcriptional inhibition IC50=7.6, 4.7, and 4.0 μM, respectively) inhibited Hh related protein (BCL2) expression. Moreover, compounds 1, 12, and 13 disrupted GLI1 and DNA complex formation. Copyright © 2015 Elsevier Ltd. All rights reserved.

  3. Chronic toxicity study of Hyptis suaveolens (L. Poit in rats

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    Bunjong Chaorai

    2005-09-01

    Full Text Available The effect of water extract of Hyptis suaveolens (H. suaveolens was evaluated for 6-month chronic toxicity in Wistar rats. Control group received distilled water orally 10 ml/kg/day. The extract was orally given to five treatment groups at the doses of 5, 50, 250, 500 and 500 mg/kg/day for 6 months. The last group was served as the recovery group. Changes in the body weights, actual and relative organ weights were not significantly demonstrated in all groups throughout the study. The results of hematological, biochemical parameters and histopathological lesions showed that the extract did not produce any significant doserelated changes. Therefore, it may be concluded that the extract of H. suaveolens at the given doses did not produce any significant toxic effect in rats during 6-month period of the treatment.

  4. Neolignans as xanthine oxidase inhibitors from Hyptis rhomboides.

    Science.gov (United States)

    Tsai, Sheng-Fa; Lee, Shoei-Sheng

    2014-05-01

    The active fraction from the EtOH extract of Hyptis rhomboides against xanthine oxidase was identified by use of an HPLC microfractionation-centrifugal vacuum evaporation-bioassay hyphenated technique. Scale-up separation of the active subfractions using semi-preparative RP-HPLC provided 13 phenylpropanoid compounds, including O-styrenylneolignans, hyprhombins A-C, epihyprhombin B, and hyprhombin B methyl ester, and O-caffeoylnorneolignans, hyprhombins D and E. All of these compounds shared a common 1,4-benzodioxane skeleton, as established by spectroscopic analyses. Hyprhombin C and epihyprhombin B exhibited better anti-xanthine oxidase activity than allopurinol, with IC50 values of 0.6 and 2.0 μM, respectively. Copyright © 2014 Elsevier Ltd. All rights reserved.

  5. Anti-staphylococcal and cytotoxic compounds from Hyptis pectinata.

    Science.gov (United States)

    Fragoso-Serrano, Mabel; Gibbons, Simon; Pereda-Miranda, Rogelio

    2005-03-01

    Bioassay-guided fractionation of a CHCl (3) extract prepared from the Mexican medicinal plant Hyptis pectinata led to the isolation of four pyrones, pectinolides A-C ( 1-3) and H ( 4). Activity was tracked using cultured KB cells. Multidrug-resistant strains of Staphylococcus aureus were sensitive to pectinolide H ( 4; KB > 20 microg/mL) in the concentration range of 32-64 microg/mL. The absolute stereochemistry of this new compound was established as 5 S-[(4 S-acetyloxy)-(1 S-hydroxy)-2 Z-octenyl]-2(5 H)-furanone on the basis of spectral, chiroptical data and chemical correlation with pectinolide A ( 1). Mosher ester derivatives were used with pectinolide B ( 2) for confirmation of the 3'-( S) absolute stereochemistry on the side chain chiral center of pectinolides A-C.

  6. EFFECT OF PICRORHIZA KURROA BENTH. IN ACUTE INFLAMMATION

    Science.gov (United States)

    Kantibiswas, Tuhin; Marjit, Bani; Maity, Lakshmi Narayan

    1996-01-01

    The effect of the indigenous drug Picrorhiza kurrooa Benth was studied on experimental acute inflammation in rats. It was observed that Picrorhiza kurrooa at a dose of 100 mg/kg b.w. has significant (p<0.01) anti inflammatory effect with respect to control, vehicle and standard drug. PMID:22556764

  7. STUDIES ON THE EFFICACY OF BRIDELIA FERRUGINEA BENTH ...

    African Journals Online (AJOL)

    ABSTRACT. The efficacy of Bridelia ferruginea Benth bark extract in wastewater treatment was investigated. Chemical analysis found the bark to contain potassium, sodium, calcium, magnesium, zinc, manganese, iron and copper. Phytochemical tests revealed the bark to contain tannins, phlobatannins, saponins, alkaloids, ...

  8. STUDIES ON THE EFFICACY OF BRIDELIA FERRUGINEA BENTH ...

    African Journals Online (AJOL)

    The efficacy of Bridelia ferruginea Benth bark extract in wastewater treatment was investigated. Chemical analysis found the bark to contain potassium, sodium, calcium, magnesium, zinc, manganese, iron and copper. Phytochemical tests revealed the bark to contain tannins, phlobatannins, saponins, alkaloids, and steroids.

  9. In vitro multiplication of Pogostemon cablin Benth. through direct ...

    African Journals Online (AJOL)

    An efficient method was developed to initiate multiple shoots from nodal explants of Pogostemon cablin Benth. MS medium supplemented with 0.5 mg/L BA initiated a mean of 45.66 shoots/nodal explant. Within 4 weeks of initiation, regenerated multiple shoots attained a height of 3.6 cm. Subsequent transfer of these in vitro ...

  10. Formulation of tablets of Xylopia parviflora benth (annonaceae ...

    African Journals Online (AJOL)

    Xylopia species are widely available in West Africa. Xylopia parviflora (Benth) plant is used in folk medicine in the management of a number of ailments, one of these is the use of the leaves in the treatment of malaria fever for which a number of patients have reported its beneficial effects. This study was designed to ...

  11. Proliferative effect of aqueous extract of Hyptis fructicosa on liver regeneration after partial hepatectomy in rats.

    Science.gov (United States)

    Lima, Sônia Oliveira; Viana, Luciano da Costa; Santana, Fábio Rafael Teixeira de; Zucoloto, Ségio; Albuquerque Junior, Ricardo Luiz de; Gomes, Margarete Zanardo

    2012-01-01

    To evaluate the effect of aqueous extract of Hyptis fructicosa on hepatic regeneration after partial hepatectomy in rats. Sixteen rats were divided in two groups: C (Control Group) and HF (Whose rats received aqueous extract of Hyptis fructicosa during 4 days using the dose of 100 mg/kg/day). On the consecutive day of this treatment, the animals of both groups underwent hepatectomy of about 67% of liver. Twenty four hours later, they were sacrificed, and the remaining mass of liver was removed and prepared to be studied through the PCNA immunohistochemical technique. The liver regeneration index of HF group was 53.56 ± 18.91%, while in C group was 21.12 ± 8.29% (p=0.0003). These results show that the administration of aqueous extract of Hyptis fructicosa using the dose of 100mg/kg/day increased the hepatocyte proliferation in the group HF.

  12. Anti-inflammatory activity of two diterpenes of Hyptis suaveolens from El Salvador.

    Science.gov (United States)

    Grassi, Paolo; Urías Reyes, Tomás S; Sosa, Silvio; Tubaro, Aurelia; Hofer, Otmar; Zitterl-Eglseer, Karin

    2006-01-01

    Separation and isolation of the two main compounds suaveolol and methyl suaveolate from leaves of chichinguaste (Hyptis suaveolens Poit., Lamiaceae) could be achieved by means of repeated column chromatography and repeated preparative thin layer chromatography. Their chemical structures were approved by MS, 1H NMR, 13C NMR and 2D-NMR experiments. The anti-inflammatory activity of the two compounds was tested for the first time as inhibition of croton oil-induced dermatitis of the mouse ear. Suaveolol and methyl suaveolate showed nearly the same dose-dependent topical anti-inflammatory activity, only two to three times lower than that of the reference drug indomethacin. The anti-inflammatory properties of these compounds could contribute to the antiphlogistic activity of extracts of Hyptis species and confirm the rational use of Hyptis suaveolens extracts in dermatological diseases.

  13. Redescubrimiento del endemismo tinerfeño Micromeria densiflora Benth. (Labiatae

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    Reyes-Betancort, Jorge Alfredo

    2011-12-01

    Full Text Available The present study confirms the existence of Micromeria densiflora Benth. on the Tenerife Island, Teno Massif, and detailed information on morphology, chorology and conservation status are provided. A natural hybrid of this species with Micromeria varia Benth. is described.Se confirma la presencia actual de Micromeria densiflora Benth. en la isla de Tenerife, en el macizo de Teno, y se aporta información sobre su caracterización morfológica, corología y estatus de conservación. Además se describe un híbrido natural de esta especie con Micromeria varia Benth.

  14. SINTESIS SENYAWA C18H26O9 DARI HIPTOLIDA HASIL ISOLASI DAUN HYPTIS PECTINATA

    OpenAIRE

    Meiny Suzery; Ely Kusniawati; Dwi Hudiyanti; Bambang Cahyono

    2012-01-01

    SYNTHESIS OF C18H26O9 COMPOUNDS FROM HYPTOLIDE ISOLATED FROM HYPTIS PECTINATA LEAVES. Isolation of hyptolide has been done from Hyptis pectinata, and alkene group transformation through oxidation reactions using H3B: OEt2 to the isolated compound was also conducted. Product analyses were carried out using TLC, UV spectrometry, IR, and LC-MS. Pure crystal with melting point of 86-87oC was isolated. The yield was 1.75% (w/w). After analysing and compilating of spectroscopic data it was confirme...

  15. [Orthogonal experiment of the extraction of triterpenoids from stems of Hyptis suaveolens with microwave].

    Science.gov (United States)

    Huang, Xiu-xiang; Lin, Cui-wu; Chen, Li-fen; Yuan, Xia; Ning, Zi-hua

    2008-01-01

    To study the best technology of the extraction of triterpenoids from the stems of Hyptis suaveolens with microwave. Orthogonal experiment was carried out to investigate 4 influential factors as follows: the time (A), the temperature (B), the solid fluid compared to (C), the NaOH density (D). The optimal conditions for microwave extraction were A1 B2 C3 D2. The microwave extraction can extract more triterpenoids from the stems of Hyptis suaveolens in shorter time with less energy. It also shows a promising prospect for leaching the effective constituents from Chinese herbal medicine by using microwave extraction.

  16. Development and characterization of novel microsatellite markers in Hyptis pectinata (Lamiaceae).

    Science.gov (United States)

    Blank, A F; Jesus, A S; Santos, C P; Grando, C; Pinheiro, J B; Zucchi, M I; Arrigoni-Blank, M F

    2014-12-04

    A microsatellite-enriched library was constructed and a set of 19 SSR markers were developed to characterize a germplasm collection of Hyptis pectinata (L.) Poit., maintained at the Universidade Federal de Sergipe (UFS). Fifteen markers of 19 ranged from moderately to highly polymorphic. A total of 113 alleles were identified, with a mean of 7.52 alleles per locus. The mean HO and HE were 0.582 and 0.657, respectively. The primers developed were efficient tools for accessing the genetic diversity of the germplasm collection analyzed and may also be useful for other studies involving this species and other species in the genus Hyptis.

  17. Cardiovascular effects of Hyptis fruticosa essential oil in rats.

    Science.gov (United States)

    Santos, M R V; Carvalho, A A; Medeiros, I A; Alves, P B; Marchioro, M; Antoniolli, A R

    2007-04-01

    In non-anesthetized normotensive rats, Hyptis fruticosa essential oil (HFEO, 5, 10, 20 and 40 mg/kg; i.v.) induced hypotension associated with tachycardia. In intact and isolated rings of rat superior mesenteric artery (control), HFEO (1-1000 microg/ml, n=6, cumulatively) induced concentration-dependent relaxations of tonus induced by 10 microM phenylephrine (Phe) (pD(2)=2.6+/-0.27; E(max)=64+/-8.3%). In denuded endothelium pre-contracted rings with Phe or K(+)-depolarizing solution (80 mM), the concentration-response curves to HFEO were not shifted (pD(2)=2.3+/-0.25 and 2.3+/-0.28, respectively), but their maximal responses were significantly (P<0.05 vs control) increased (E(max)=122.3+/-18.2% and 92+/-3.6%, respectively). HFEO was also capable of antagonizing the concentration-response curves to CaCl(2) (3 microM-30 mM) in a dose-dependent manner.

  18. [Chemical constituents of Hyptis rhomboidea and their antifungal activity].

    Science.gov (United States)

    Tang, Lu; Li, Xi-Feng; Yang, Sheng-Xiang; Qiu, Yan; Yuan, Ke

    2014-06-01

    The present work is to investigate the chemical constitutions of Hyptis rhomboidea and their antifungal activities. The compounds were isolated by Toyopearl HW-40, Sephadex LH-20, MCI-Gel CHP-20, RP-18, PTLC and silica column chromatographic methods and subjected to evaluate some monomers antifungal activity of eight kinds of plant pathogenic bacteria. Eleven compounds were isolated and identified as ethyl caffeate (1), ursolic acid (2), oleanolic acid (3), vanillactic acid (4), methyl rosmarinate (5), kaempferol 3-O-alpha-L-rhamnopyranosyl-(1 --> 6) -beta-D-glucopyranoside (6), kaempferol 3-O-alpha-L-rhamnopyranosyl-(1 --> 6)-beta-D-glucopyranoside (7), ilexgenin A (8), beta-amyrin (9), kaempferol 3-O-beta-D-glucopyranoside (astrgalin, 10) and cholest-5-ene-3beta, 4beta-diol (11). Compound 1 showed the strongest inhibitory effect on Sclerotinia sclerotiorum with the MIC 16.2 mg x L(-1), and compound 5 showed the strongest inhibitory effect on S. minor and Exserohilum turcicum with MIC 16.2, 8.1 mg x L(-1), respectively. All compounds were isolated from the H. rhomboidea for the first time, and compounds 1 and 5 showed antifungal activity.

  19. Suaveolic Acid: A Potent Phytotoxic Substance of Hyptis suaveolens

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    A. K. M. Mominul Islam

    2014-01-01

    Full Text Available Hyptis suaveolens (Lamiaceae is an exotic invasive plant in many countries. Earlier studies reported that the aqueous, methanol, and aqueous methanol extract of H. suaveolens and its residues have phytotoxic properties. However, to date, the phytotoxic substances of this plant have not been reported. Therefore, the objectives of this study were isolation and identification of phytotoxic substances of H. suaveolens. Aqueous methanol extract of this plant was purified by several chromatographic runs through bioassay guided fractionation using garden cress (Lepidium sativum as a test plant. Final purification of a phytotoxic substance was achieved by reverse phase HPLC and characterized as 14α-hydroxy-13β-abiet-8-en-18-oic acid (suaveolic acid by high-resolution ESI-MS, 1H-,13C-NMR, CD, and specific rotation. Suaveolic acid inhibited the shoot growth of garden cress, lettuce (Lactuca sativa, Italian ryegrass (Lolium multiflorum, and barnyard grass (Echinochloa crus-galli at concentrations greater than 30 µM. Root growth of all but lettuce was also inhibited at concentrations greater than 30 µM. The inhibitory activities were concentration dependent. Concentrations required for 50% growth inhibition of suaveolic acid for those test plant species were ranged from 76 to 1155 µM. Therefore, suaveolic acid is phytotoxic and may be responsible for the phytotoxicity of H. suaveolens plant extracts.

  20. Suaveolic acid: a potent phytotoxic substance of Hyptis suaveolens.

    Science.gov (United States)

    Islam, A K M Mominul; Ohno, Osamu; Suenaga, Kiyotake; Kato-Noguchi, Hisashi

    2014-01-01

    Hyptis suaveolens (Lamiaceae) is an exotic invasive plant in many countries. Earlier studies reported that the aqueous, methanol, and aqueous methanol extract of H. suaveolens and its residues have phytotoxic properties. However, to date, the phytotoxic substances of this plant have not been reported. Therefore, the objectives of this study were isolation and identification of phytotoxic substances of H. suaveolens. Aqueous methanol extract of this plant was purified by several chromatographic runs through bioassay guided fractionation using garden cress (Lepidium sativum) as a test plant. Final purification of a phytotoxic substance was achieved by reverse phase HPLC and characterized as 14α-hydroxy-13β-abiet-8-en-18-oic acid (suaveolic acid) by high-resolution ESI-MS, (1)H-,(13)C-NMR, CD, and specific rotation. Suaveolic acid inhibited the shoot growth of garden cress, lettuce (Lactuca sativa), Italian ryegrass (Lolium multiflorum), and barnyard grass (Echinochloa crus-galli) at concentrations greater than 30 µM. Root growth of all but lettuce was also inhibited at concentrations greater than 30 µM. The inhibitory activities were concentration dependent. Concentrations required for 50% growth inhibition of suaveolic acid for those test plant species were ranged from 76 to 1155 µM. Therefore, suaveolic acid is phytotoxic and may be responsible for the phytotoxicity of H. suaveolens plant extracts.

  1. Dormancy break in Pithecellobium dulce (Roxb. Benth. seeds

    Directory of Open Access Journals (Sweden)

    Francisco Elder Carlos Bezerra Pereira

    2015-02-01

    Full Text Available This research aimed to evaluate different methods for breaking dormancy in seeds of Pithecellobium dulce (Roxb. Benth., arboreal species of multiple use in the semiarid region of Northeast Brazil. The treatments were: control – intact seeds (T1; immersion in water until it reaches 80 °C (T2, and 100 °C (T3, concentrated sulfuric acid for five minutes (T4, 10 minutes (T5 , and 15 minutes (T6, mechanical scarification with sandpaper number 80 (T7, mechanical scarification with sandpaper number 80 + immersion in water for six hours (T8, and for 12 hours (T9, soaking in water for 24 hours (T10, and for 48 hours (T11. The characteristics evaluated were such: emergency, mean emergency time, shoot length, root length, number of leaves, leaf area and total dry matter. The best method for breaking dormancy of seeds of Pithecellobium dulce (Roxb. Benth. is mechanical scarification with sandpaper number 80.

  2. A survey of witchweed [ Striga hermonthica (Del.) benth.] on arable ...

    African Journals Online (AJOL)

    A survey of witchweed [Striga hermonthica (Del.) benth.] on arable and lands in Nunlan Local Government area of Adamawa state, Nigeria. YA Jesse, MY Jada, H Sule, CB Phillip. Abstract. No Abstract. Ife Journal of Science Vol. 7(2) 2005: 319-321. http://dx.doi.org/10.4314/ijs.v7i2.32191 · AJOL African Journals Online.

  3. Volatile Components of Oiti Fruit (Licania tomentosa Benth.

    Directory of Open Access Journals (Sweden)

    Maria A. C. Kaplan

    2010-10-01

    Full Text Available The fresh fruits from Licania tomentosa Benth. (Chrysobalanaceae , were submitted to hydrodistillation in a modified Clevenger-type apparatus. Analysis of volatile oil were performed by GC and GC-MS. Comparison of results with literature data allowed the chemical characterization of 83% of compounds of mixture. Thirteen compounds were identified, three monoterpenes, five aliphatic esters, two alcohols, two ketones and one aldehyde. The alcohols were the major compounds (35.6%, but the esters showed great diversity.

  4. The genus Isodon (Schrad. ex Benth. Spach in Africa and a new genus Rabdosiella Codd (Lamiaceae

    Directory of Open Access Journals (Sweden)

    L. E. Codd

    1984-12-01

    Full Text Available The typification of the genus Isodon (Schrad. ex Benth. Spach and its occurrence in Africa are discussed; an allied genus Rabdosiella Codd is described and the combinations R. calycina (Benth. Codd and R. ternifolia (D.Don Codd (the latter an Indian species are effected.

  5. Evaluation of ovicidal and larvicidal effects of leave extracts of Hyptis ...

    African Journals Online (AJOL)

    Evaluation of ovicidal and larvicidal effects of leave extracts of Hyptis suaveolens (L) POIT (Lamiaceae) against Anopheles gambiae (Diptera: Anophelidae) ... 67.04) and 80.02 (range 77.55 – 86.41) respectively thus making both extracts candidates for further fractionation and compound isolation studies to characterize the ...

  6. In vivo antimalarial activity of the ethanolic leaf extract of Hyptis ...

    African Journals Online (AJOL)

    In vivo antimalarial activity of the ethanolic leaf extract of Hyptis suaveolens poit on Plasmodium berghei in Mice. ... On day 7 the red blood cells (RBC), white blood cells (WBC), haemoglobin (Hb) and packed cell volume (PCV) were evaluated. The extract demonstrated dose-dependent inhibition effect on the parasites with ...

  7. Novel cytotoxic isolated from Jamaican Hyptis verticillata jacq induces apoptosis and overcomes multidrug resistance

    National Research Council Canada - National Science Library

    White, Yohann; Hamada, Toshiyuki; Yoshimitsu, Makoto; Nakashima, Mitsuyoshi; Hachiman, Miho; Kozako, Tomohiro; Matsushita, Kakushi; Uozumi, Kimiharu; Suzuki, Shinsuke; Kofune, Hiroki; Furukawa, Tatsuhiko; Arima, Naomichi

    2011-01-01

    ...-(3,4,5-trimethoxyphenyl)-8, 9 - dihydrofuro[3',4':6,7]naphtho[2,3-d][1,3]dioxol-6(5H)-one (4-MTDND) from the Jamaican plant Hyptis verticillata jacq, and its effects on apoptosis, cell cycle and drug resistance were elucidated...

  8. an evaluation of the toxic effects of hyptis suaveolens in rats

    African Journals Online (AJOL)

    ... were found to include saponin, tannin and flavonoids. Histopathological examination of the liver revealed fatty changes and congestion of the central vein as the major indicator of hepatic damage. Key Words: Hyptis suaveolens; Labiatae; rats; toxicity. Nigerian Journal of Natural Products and Medicine Vol.7 2003: 49-52 ...

  9. Antiurolithiatic activity of coleus Aromaticus Benth. In Rats

    OpenAIRE

    Ghosh, R.B.; T. K. Sur; Maity, L.N.; Chakraborty, S.C

    2000-01-01

    Water extract of the leaves of Coleus aromaticus  Benth. Was tested for its antiurolithiatic activity against calcium oxalate stones in male albino rats. Calcium oxalate stones were induced by feeding 3% w/w sodium oxalate along with normal feed. The water extract of coleus aromaticus (0.5 g/kg and 1.0 g/kg, once, orally for 30 days) was found to be effective in reducing deposition of calcium oxalate. Hence, it is suggested, coleus aromaticus leaves may be effective in the therapy of calcium ...

  10. Diuretic activity of coleus aromaticus benth on rats.

    Science.gov (United States)

    Sur, T K; Pandit, S; Biswas, T K; Ghosh, R B; Bhattacharyya, D

    2003-04-01

    Water extract of the leaves of Coleus Aromaticus Benth was tested for its diuretic activity in male albino rats. The study was carried out on normal rats using furosemide as a standard reference drug. Rats were treated with furosemide (4 mg/kg. p.o) and Coleus aromaticus (0.5 g/kg and 1.0 g/kg, p/o). Urine was collected and its volume was recorded. Urinary levels of sodium, potassium and chloride were estimated. Treatment with Coleus aromaticus produced diuresis. The urine output and electrolytes concentration was significantly increased. Hence, it is suggested, Coleus aromaticus leaves has diuretic activity on rats.

  11. DIURETIC ACTIVITY OF COLEUS AROMATICUS BENTH ON RATS

    OpenAIRE

    T. K. Sur; S. Pandit; Biswas, T.K.; Ghosh, R.B.; Bhattacharyya, D.

    2003-01-01

    Water extract of the leaves of Coleus Aromaticus Benth was tested for its diuretic activity in male albino rats. The study was carried out on normal rats using furosemide as a standard reference drug. Rats were treated with furosemide (4 mg/kg. p.o) and Coleus aromaticus (0.5 g/kg and 1.0 g/kg, p/o). Urine was collected and its volume was recorded. Urinary levels of sodium, potassium and chloride were estimated. Treatment with Coleus aromaticus produced diuresis. The urine output and electrol...

  12. Diploflavone, a New Flavonoid from Diplotropis ferruginea Benth. (Fabaceae)

    Energy Technology Data Exchange (ETDEWEB)

    Almeida, Jackson Roberto G.S. [Universidade Federal do Vale do Sao Francisco, Petrolina, PE (Brazil); Barbosa-Filho, Jose Maria; Cabral, Analucia G.S.; Agra, Maria de Fatima; Silva, Marcelo S. da [Universidade Federal da Paraiba, Joao Pessoa, PB (Brazil). Lab. de Tecnologia Farmaceutica]. E-mail: jbarbosa@ltf.ufpb.br; Da Cunha, Emidio V. Leitao [Universidade Estadual da Paraiba, Campina Grande, PB (Brazil). Dept. de Farmacia; Nascimento, Silene C. do [Universidade Federal de Pernambuco, Recife, PE (Brazil). Dept. de Antibioticos; Braz-Filho, Raimundo [Universidade Estadual do Norte Fluminense (UENF), Campos dos Goytacases, RJ (Brazil). Setor de Quimica de Produtos Naturais. Lab. de Ciencias Quimicas

    2005-11-15

    The chemical examination of Diplotropis ferruginea Benth. resulted in the isolation of a new 3-methoxyflavone, 3-methoxy-6-O-prenyl-6,6-dimethylchromene-(7,8,2,3)-flavone, to which was given the trivial name diploflavone (1); as well as the known 3,6-dimethoxy-6,6- dimethylchromene-(7,8,2,3)-flavone (2). The structure of the new compound was established by spectral analyses. Cytotoxic activity of the isolated compounds was tested against the cells NClH292 (lung carcinoma), HEp-2 (larynx carcinoma) and KB (oral epidermoid carcinoma). The cells HEp-2 were the most affected by the substances tested. (author)

  13. Phytochemical and Bioactivity Evaluation of Scrophularia amplexicaulis Benth.

    Directory of Open Access Journals (Sweden)

    Ardalan Pasdaran

    2016-01-01

    Full Text Available Scrophularia amplexicaulis Benth. is an Iranian endemic species of the genus Scrophularia, which comprises ca. 200 medicinally important herbaceous flowering plants . Phytochemical investigation of the methanol extract of the aerial parts of this species afforded two iridoid glycosides, scropolioside D (1 and scrophuloside B 4 (2, and two phenylalkanoid glycosides, salidroside (3 and verbascoside (4. S tructures of these compounds were determined by comprehensive spectroscopic analyses. Free-radical-scavenging activity, potential antimalarial property, and contact toxicity as well as general toxicity of the extract and fractions were assessed.

  14. Chemical composition and antimicrobial activity of the essential oil of Hyptis pectinata (l. Poit.

    Directory of Open Access Journals (Sweden)

    Patrícia O. Santos

    2008-01-01

    Full Text Available Essential oil was extracted from leaves of Hyptis pectinata using hydrodistillation, and its composition determined using GC-FID and GC-MS. Chemical analysis showed that there was a predominance of sesquiterpenes, of which β-caryophyllene (18.34%, caryophyllene oxide (18.00% and calamusenone (24.68% were measured for the first time in the genus Hyptis. Twenty-one compounds were identified, and calamusenone was isolated using preparative thin layer chromatography with a silica gel plate (60 PF254. The minimal inhibitory concentration (MIC and minimal microbicidal concentration (MMC were determined for various pathogenic microorganisms. H. pectinata oil was most effective against Gram (+ bacteria and yeasts.

  15. Chemical composition and antimicrobial activity of the essential oil of Hyptis pectinata (l.) Poit

    Energy Technology Data Exchange (ETDEWEB)

    Santos, Patricia O.; Costa, Marcilene de J. C.; Alves, Jose A.B.; Nascimento, Paula F.C.; Melo, Dangelly L.F.M. de; Barbosa Junior, Antonio M.; Trindade, Rita de C. [Universidade Federal de Sergipe, Aracaju, SE (Brazil). Dept. de Morfologia]. E-mail: ritinhat@hotmail.com; Blank, Arie F. [Universidade Federal de Sergipe, Aracaju, SE (Brazil). Dept. de Engenharia Agronomica; Arrigoni-Blank, Maria F. [Nucleo de Ciencias Biologicas, Itabaiana, SE (Brazil). Campus Prof. Alberto Carvalho; Alves, Pericles B. [Universidade Federal de Sergipe, Aracaju, SE (Brazil). Dept. de Quimica; Nascimento, Maria da Paz F. do [Universidade Federal de Sao Carlos, SP (Brazil). Dept. de Quimica

    2008-07-01

    Essential oil was extracted from leaves of Hyptis pectinata using hydrodistillation, and its composition determined using GC-FID and GC-MS. Chemical analysis showed that there was a predominance of sesquiterpenes, of which b-caryophyllene (18.34%), caryophyllene oxide (18.00%) and calamusenone (24.68%) were measured for the first time in the genus Hyptis. Twenty-one compounds were identified, and calamusenone was isolated using preparative thin layer chromatography with a silica gel plate (60 PF{sub 254}). The minimal inhibitory concentration (MIC) and minimal microbicidal concentration (MMC) were determined for various pathogenic microorganisms. H. pectinata oil was most effective against Gram (+) bacteria and yeasts. (author)

  16. Chemical Variability and Biological Activities of Volatile Oils from Hyptis suaveolens (L.) Poit.

    OpenAIRE

    Luiz Claudio Barbosa; Felipe Terra Martins; Róbson Ricardo Teixeira; Marcelo Polo; Ricardo Marques Montanari

    2013-01-01

    Hyptis suaveolens (L.) Poit. belongs to the Lamiaceae family and is widely used in folk medicine in various countries. Th e essential oils from H. suaveolens have been extensively investigated and are mainly composed of monoterpenes and sesquiterpenes, although significant diterpene content has been reported in recent studies. The survey of the literature concerning H. suaveolens essential oils revealed a high level of chemical variability in terms of quantity and composition that is c...

  17. Identification of Secretory Structure, Histochemistry and Phytochemical Compounds of Medicinal Plant Hyptis Capitata Jacq.

    OpenAIRE

    Rupa, Darius; Sulistyaningsih, Yohana Caecilia; Dorly, Dorly; Ratnadewi, Diah

    2017-01-01

    Hyptis capitata Jacq. (common name: Knobweed or False Ironwort) belongs to Lamiaceae family and is among known traditional medicine. The Anak Dalam Tribe of Jambi Province uses the leaves of H. capitata to cure external and internal wounds. This study was aimed at identifying and analyzing secretory structure, histochemistry and phytochemical content of H. capitata leaves. The results showed that H. capitata leaves have secretory structures in the form of peltate, capitate and uniseriate gla...

  18. Anti-Inflammatory Effects of Hyptis albida Chloroform Extract on Lipopolysaccharide-Stimulated Peritoneal Macrophages

    OpenAIRE

    Sánchez Miranda, Elizabeth; Pérez Ramos, Julia; Fresán Orozco, Cristina; Zavala Sánchez, Miguel Angel; Pérez Gutiérrez, Salud

    2013-01-01

    We examined the effects of a chloroform extract of Hyptis albida (CHA) on inflammatory responses in mouse lipopolysaccharide (LPS) induced peritoneal macrophages. Our findings indicate that CHA inhibits LPS-induced production of tumor necrosis factor (TNF- ? ) and interleukin-6 (IL-6). During the process, levels of cyclooxygenase-2 (COX-2), nitric oxide synthase (iNOS), and nitric oxide (NO) increased in the mouse peritoneal macrophages; however, the extract suppressed them significantly. The...

  19. Hyptis verticillata Jacq: a review of its traditional uses, phytochemistry, pharmacology and toxicology.

    Science.gov (United States)

    Picking, D; Delgoda, R; Boulogne, I; Mitchell, S

    2013-05-02

    Hyptis verticillata Jacq. (Lamiaceae) (John Charles) is an important medicinal plant with a long history of traditional use, originating in Central America and now extending from Florida to Colombia and across the Caribbean. Records of its earliest use date back to the ancient Mayan and Aztec cultures of Mesoamerica. There is no indication that this plant is being used outside of the Americas. This review aims to provide a comprehensive overview of the traditional use, phytochemistry, pharmacological activity and toxicology of Hyptis verticillata and to highlight the opportunities for greater development of the plant's medicinal properties at a local and international level. An extensive and systematic review of the literature was undertaken and all relevant abstracts and full-text articles analysed and included in the review. A wide range of traditional uses are cited in the literature, from internal uses for conditions affecting the respiratory system, digestive tract and gynaecological system to external uses for conditions affecting the skin and musculoskeletal system. Pharmacological studies to date have demonstrated significant activity which support the traditional use of the plant as an antiinflammatory, antimicrobial, antisecretory agent and hormone modulator. In addition studies have identified anti-cancer, acaricidal, insecticidal and molluscicidal activity. No clinical trials had been completed at the time of this review. A number of key phytochemicals have been isolated, identified and published to date including: 17 lignans; 4 triterpenes; 11 diterpenes, 3 sesquiterpenes, 3 monoterpenes, 2 flavonoids, 1 polyphenol and 1 alkaloid. Nine of these phytochemicals are novel to Hyptis verticillata. Plant extracts and isolated phytochemicals exhibit a broad range of activities that include: antimitotic; antiproliferative; cytotoxic; antioxidant; antiinflammatory; antibacterial; antifungal; antiviral; anti-HIV; antisecretory; hepatoprotective; insecticidal and

  20. Atividade hemaglutinante em sementes de Dioclea rostrata Benth

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    T.B Grangeiro

    1990-01-01

    Full Text Available Sementes de Dioclea rostrata Benth. Têm uma lectina que é melhor extraída em tampão acetato de sódio a pH 4,0 e é seletivamente adsorvida em Sephadex G-50, apresentando um alto título de hemaglutinação contra eritrócitos de coelho. A lectina também aglutina inespecificamente hemácias de carneiro e humanas. A atividade hemaglutinante é inibida por glicose, frutose, manose e alfa-metil-D-manosídeo, sendo os dois últimos os mais potentes inibidores. A lectina é uma metaloproteína, uma vez que a atividade hemaglutinamente desaparece na presença de EDTA.Seeds of Dioclea rostrata Benth. Have a lectin which is best extracted with Na-acetate buffer at pH 4.0 and that is selectively adsorbed in a Sephadex G-50 matrix, yielding a protein fraction of high hemagg lutinating titre against rabbit erythrocytes. It also agglutinates sheep, and human cells inespecifically. Its hemagglutinating activity is inhibited by glucose fructose, mannose and O-methyl-D-mannopyranoside the latter two being the most active inhibitors. The lectin seems to be a metaloprotein since the hemagglutinating activity is lost in the presence of EDTA.

  1. Hyptis suaveolens (L.) Poit (Lamiaceae), a medicinal plant protects the stomach against several gastric ulcer models.

    Science.gov (United States)

    Jesus, N Z T; Falcão, H S; Lima, G R M; Caldas Filho, M R D; Sales, I R P; Gomes, I F; Santos, S G; Tavares, J F; Barbosa-Filho, J M; Batista, L M

    2013-12-12

    Hyptis suaveolens is used by the traditional population in several parts of the world to treat inflammation, gastric ulcer and infection and is used as a crude drug to relieve symptoms related with gastric ulcer or gastritis in northeaster and central region of Brazil. the standardized ethanolic extract (Hs-EtOHE) and hexanic fraction (Hs-HexF) of Hyptis suaveolens (62,5, 125, 250 and 500 mg/kg) was evaluated in several models of acute gastric ulcers. The participation of NO was evaluated by pretreatment with L-NAME and non-protein sulfyhydryls by NEM in the gastroprotective effect. Hs-EtOHE and Hs-HexF markedly reduced the gastric lesions induced by all ulcerogenic agents (HCl/ethanol, ethanol, NSAIDs and hypothermic restraint-stress). Gastric ulcerations were exacerbated by administration of NEM suggesting that the gastroprotective mechanism of action of Hs-EtOHE and Hs-HexF involves sulfhydryl groups. Ours results show that an extract of Hyptis suaveolens, administered orally to rodents, present gastro protective activity in different models of acute of gastric ulcer and give some support to the reported claims on the use of this plant as a gastro protective agent. © 2013 Published by Elsevier Ireland Ltd.

  2. QUANTITATIVE DETERMINATION OF THE AMOUNT OF FLAVONOIDS IN THE HERB OF SALVIA FARINACEA BENTH.

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    O. I. Popova

    2016-01-01

    Full Text Available Chromatographic researches conducted have allowed determination of the flavonoids presence in raw materials of Salvia farinacea Benth. They were rutin, quercetin, hyperoside, and 5 phenol carbonic acids: caffeic, p-coumaric, ferulic, gallic, and cinnamic acids. The quantitative content of the sum of flavonoids in Salvia farinacea Benth. was conducted by the method of differential spectrophotometry, and it amounted to 0.55-0.60% in terms of rutin. The research conducted made it possible to expand the scientific data of phenolic compounds of Salvia farinacea Benth. and to offer the possibility of recycling, monitoring procurement and targeted use of Salvia farinacea Benth. herb in pharmacy and medicine.

  3. Antiplasmodial activity of sesquiterpene lactones and a sucrose ester from Vernonia guineensis Benth. (Asteraceae)

    National Research Council Canada - National Science Library

    Toyang, Ngeh J; Krause, Michael A; Fairhurst, Rick M; Tane, Pierre; Bryant, Joseph; Verpoorte, Rob

    2013-01-01

    Aqueous preparations of Vernonia guineensis Benth. (Asteraceae) are used in Cameroonian folk medicine as a general stimulant and to treat various illnesses and conditions including malaria, bacterial infections and helminthic infestations...

  4. Cytotoxic sesquiterpene lactones from the leaves of Vernonia guineensis Benth. (Asteraceae)

    National Research Council Canada - National Science Library

    Toyang, Ngeh J; Wabo, Hippolyte K; Ateh, Eugene N; Davis, Harry; Tane, Pierre; Sondengam, Luc B; Bryant, Joseph; Verpoorte, Rob

    2013-01-01

    Vernonia guineensis Benth. (Asteraceae) preparations are used in folk medicine in Cameroon to treat a number of ailments, including prostate cancer and malaria, and is used as an anthelmintic, adaptogen and antidote...

  5. Gastroprotective effect of alpha-pinene and its correlation with antiulcerogenic activity of essential oils obtained from Hyptis species.

    Science.gov (United States)

    Pinheiro, Marcelo de Almeida; Magalhães, Rafael Matos; Torres, Danielle Mesquita; Cavalcante, Rodrigo Cardoso; Mota, Francisca Sheila Xavier; Oliveira Coelho, Emanuela Maria Araújo; Moreira, Henrique Pires; Lima, Glauber Cruz; Araújo, Pamella Cristina da Costa; Cardoso, José Henrique Leal; de Souza, Andrelina Noronha Coelho; Diniz, Lúcio Ricardo Leite

    2015-01-01

    Alpha-pinene (α-pinene) is a monoterpene commonly found in essential oils with gastroprotective activity obtained from diverse medicinal plants, including Hyptis species. The genus Hyptis (lamiaceae) consists of almost 400 species widespread in tropical and temperate regions of America. In the north and northeastern Brazil, some Hyptis species are used in traditional medicine to treat gastrointestinal disturbances. The present study has investigated the gastoprotective effect of purified α-pinene in experimental gastric ulcer induced by ethanol and indomethacin in mice. Gastric ulcers were induced in male Swiss mice (20-30 g) by oral administration of absolute ethanol or indomethacin 45 min after oral pretreatment with vehicle, standard control drugs or α-pinene (10, 30, and 100 mg/kg). One hour after the ulcerative challenges, the stomach were removed, and gastric lesions areas measured. The effects of α-pinene on the gastric juice acidity were determined by pylorus ligation model. The gastrointestinal motility and mucus depletion were determined by measuring the gastric levels of phenol red and alcian blue, respectively. Hematoxylin and eosin stained sections of gastric mucosa of the experimental groups were used for histology analysis. α-pinene pretreatment inhibited ethanol-induced gastric lesions, reduced volume and acidity of the gastric juice and increased gastric wall mucus (P Hyptis species (P Pearson = 0.98). Our data showed that the α-pinene exhibited significant antiulcerogenic activity and a great correlation between concentration of α-pinene and gastroprotective effect of Hyptis species was also observed.

  6. In vitro and in vivo determination of antioxidant activity and mode of action of isoquercitrin and Hyptis fasciculata.

    Science.gov (United States)

    Silva, C G; Raulino, R J; Cerqueira, D M; Mannarino, S C; Pereira, M D; Panek, A D; Silva, J F M; Menezes, F S; Eleutherio, E C A

    2009-08-01

    Reactive oxygen species (ROS) are thought to underline the process of ageing and the pathogenicity of various diseases, such as neurodegenerative disorders and cancer. The use of traditional medicine is widespread and plants still present a large source of natural antioxidants that might serve as leads for the development of novel drugs. In this paper, the alcoholic extract from leaves of Hyptis fasciculata, a Brazilian medicinal plant, and isoquercitrin, a flavonoid identified in this species, showed to be active as 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavengers. The extract of Hyptis fasciculata and isoquercitrin were also able to increase tolerance of the eukaryotic microorganism Saccharomyces cerevisiae to both hydrogen peroxide and menadione, a source of superoxide. Cellular protection was correlated with a decrease in oxidative stress markers, such as levels of ROS, protein carbonylation and lipid peroxidation, confirming the antioxidant potential of Hyptis fasciculata and isoquercitrin.

  7. ESTUDO TAXONÔMICO DE HYPTIS, SECÇÃO PELTODON HARLEY & J. F. B. PASTORE (LAMIACEAE)

    OpenAIRE

    de Souza Bispo, Thayná; Faculdade Araguaia.; Faria, Maria Tereza; Faculdade Araguaia; Dias Ferreira, Heleno; Universidade Federal de Goiás

    2014-01-01

    Neste trabalho apresenta-se o tratamento taxonômico da secção Peltodon Harley & J. F. B. Pastore do gênero Hyptis Jacq., como parte do projeto “Estudo das espécies do gênero Hyptis Jacq. (Lamiaceae) ocorrentes em Goiás”. Após estudos morfológicos com auxílio de bibliografias especializadas, complementadas pela análise de espécimes de herbários,realizaram-se as identificações, descrições e ilustrações botânicas. A seção está representada no Brasil por cinco espécies: Hyptis campestris Harl...

  8. Biometria de frutos e sementes e germinação de Plathymenia reticulata benth. e Plathymenia foliolosa benth. (Fabaceae - mimosoideae Biometry of fruits and seeds and germination of Plathymenia reticulata benth. and Plathymenia foliolosa benth. (Fabaceae - mimosoideae

    Directory of Open Access Journals (Sweden)

    Renata de Melo Ferreira Lopes

    2010-10-01

    Full Text Available Este estudo objetivou comparar características biométricas de frutos e sementes e o efeito de diferentes escarificações, temperaturas e luz na germinação de Plathymenia reticulata Benth. e Plathymenia foliolosa Benth. Foram registrados comprimento, largura, espessura, massa da matéria seca e fresca de frutos (n = 100 e sementes (n = 100 de cada espécie. Os diferentes tratamentos foram escarificações mecânica e química e temperaturas (fotoperíodo/nictoperíodo de 20, 30 e 35/15 °C (12/12 e 0/24 e 25 e 35 °C (12/12. Os frutos de P. foliolosa mostraram-se mais largos, espessos e pesados e as sementes, mais compridas e espessas do que as de P. reticulata. As sementes de ambas as espécies não apresentaram fotoblastismo. A escarificação ácida não aumentou significativamente a germinabilidade das sementes em relação ao grupo-controle, enquanto a escarificação mecânica incrementou significativamente a germinabilidade apenas de P. foliolosa. As germinabilidades a 25 °C das sementes de P. reticulata intactas, escarificadas com ácido e lixa foram, respectivamente, de 55%, 60% e 89%. Para as sementes de P. foliolosa esses valores foram 48%, 37,5% e 83%, respectivamente.Esses resultados apontam limitações na germinação de P. foliolosa impostas pelo tegumento, entretanto o efeito deste restringindo a germinação das sementes intactas decresceu com a elevação da temperatura.This study aimed to compare the biometry of fruits and seeds, as well as the effect of different scarification, temperature and light conditions on the germination of Plathymenia reticulata Benth. and Plathymenia foliolosa Benth. We measured the length, width, thickness, and fresh mass of fruits (n= 100 and seeds (n= 100 of each specie. The different treatments were mechanic and acid scarification, temperatures (photoperiod/nictoperiod of 20, 30 and 35/15 °C (12/12 and 0/24 and 25 and 35 °C (12/12. P. foliolosa fruits were wider, thickener and heavier

  9. Biological activity and chemical composition of the essential oil from Jamaican Hyptis verticillata Jacq.

    Science.gov (United States)

    Facey, Petrea C; Porter, Roy B R; Reese, Paul B; Williams, Lawrence A D

    2005-06-15

    The chemical composition of the essential oil obtained by hydrodistillation from the aerial parts of Hyptis verticillata Jacq. was elucidated by a combination of GC and GC-MS analyses. The oil was dominated by the sesquiterpenoids cadina-4,10(15)-dien-3-one (15.1%) (1) and aromadendr-1(10)-en-9-one (squamulosone) (30.7%) (2). The oil exhibited chemosterilant activities against the cattle tick, Boophilus microplus Canest., and toxic action against adult Cylas formicarius elegantulus Summer, the most destructive pest of sweet potato (Ipomoea species).

  10. Antinociceptive effect and acute toxicity of the Hyptis suaveolens leaves aqueous extract on mice.

    Science.gov (United States)

    Santos, Thiago C; Marques, Maxsuel S; Menezes, Igor A C; Dias, Kellyane S; Silva, Aline B L; Mello, Iderjane C M; Carvalho, Ana C S; Cavalcanti, Sócrates C H; Antoniolli, Angelo R; Marçal, Rosilene M

    2007-07-01

    The aqueous extract of Hyptis suaveolens leaves was studied for their antinociceptive property in chemical and thermal models of nociception in mice. Oral administration of the aqueous extract (100, 200, and 400 mg/kg) dose-dependently reduced the number of writhings induced by acetic acid, decreased the licking activity of the early phase in formalin test and increased the reaction time in hot-plate test. The antinociceptive effect was significantly antagonized by naloxone (3 mg/kg; i.p.). Preliminary acute toxicity study showed that no animal death with doses up to 5 g/kg (p.o.).

  11. Anti-Inflammatory Effects of Hyptis albida Chloroform Extract on Lipopolysaccharide-Stimulated Peritoneal Macrophages.

    Science.gov (United States)

    Sánchez Miranda, Elizabeth; Pérez Ramos, Julia; Fresán Orozco, Cristina; Zavala Sánchez, Miguel Angel; Pérez Gutiérrez, Salud

    2013-01-01

    We examined the effects of a chloroform extract of Hyptis albida (CHA) on inflammatory responses in mouse lipopolysaccharide (LPS) induced peritoneal macrophages. Our findings indicate that CHA inhibits LPS-induced production of tumor necrosis factor (TNF- α ) and interleukin-6 (IL-6). During the process, levels of cyclooxygenase-2 (COX-2), nitric oxide synthase (iNOS), and nitric oxide (NO) increased in the mouse peritoneal macrophages; however, the extract suppressed them significantly. These results provide novel insights into the anti-inflammatory actions of CHA and support its potential use in the treatment of inflammatory diseases.

  12. Antinociceptive effect and acute toxicity of the essential oil of Hyptis fruticosa in mice.

    Science.gov (United States)

    Menezes, Igor A C; Marques, Maxsuel S; Santos, Thiago C; Dias, Kellyane S; Silva, Aline B L; Mello, Iderjane C M; Lisboa, Ana C C D; Alves, Péricles B; Cavalcanti, Sócrates C H; Marçal, Rosilene M; Antoniolli, Angelo R

    2007-04-01

    The essential oil of the Hyptis fruticosa leaves was analyzed by GC/MS and evaluated for antinociceptive property as well as acute toxicity in mice. The essential oil, at doses of 100, 200, and 400 mg/kg (s.c.), produced significant inhibition of acetic acid-induced writhing, but did not manifest a significant effect in hot-plate test. There was no acute toxicity at doses up to 5 g/kg. Bicyclogermacrene, 1,8-cineole, alpha-pinene, and beta-caryophyllene were the major compounds detected in the essential oil.

  13. Essential Oil Composition and Antibacterial Activity of Hyptis colombiana from the Venezuelan Andes.

    Science.gov (United States)

    Flores, Mayalin; Rojas, Luis; Aparicio, Rosa; Lucena, María Eugenia; Usubillaga, Alfredo

    2015-10-01

    Hyptis colombiana is an aromatic shrub native to the Colombian and Venezuelan Andes. Aerial parts were collected in Mérida State at about 3100 m above sea level in February 2005, and May and October 2006. The essential oil was found to contain germacrene D and β-caryophyllene as main constituents (about 50%). The February 2005 and October 2006 oils were found to have antibacterial activity against Staphylococcus aureus, but not the May 2006 oil, probably due to the lack of some minor constituent.

  14. Gastroprotective and ulcer healing effects of essential oil from Hyptis spicigera Lam. (Lamiaceae).

    Science.gov (United States)

    Takayama, Christiane; de-Faria, Felipe Meira; de Almeida, Ana Cristina Alves; Valim-Araújo, Deborah de Arantes e Oliveira; Rehen, Camilla Souza; Dunder, Ricardo José; Socca, Eduardo Augusto Rabelo; Manzo, Luis Paulo; Rozza, Ariane Leite; Salvador, Marcos José; Pellizzon, Claúdia Helena; Hiruma-Lima, Clélia Akiko; Luiz-Ferreira, Anderson; Souza-Brito, Alba Regina Monteiro

    2011-04-26

    Hyptis Jacq. (Lamiaceae) is being used in traditional medicine to treat fever, inflammation and gastric disturbances. Hyptis spicigera Lam. is a native plant distributed across the central region of Brazil. The essential oil extracted from this plant is used in folk medicine as antipyretic. The effects of the essential oil obtained from the aerial parts of Hyptis spicigera (OEH) were evaluated for their gastroprotective and healing activities. OEH chemical composition was analyzed by gas chromatography-mass spectrometry (GC-MS). The gastroprotective action of the OEH was evaluated in rodent experimental models (ethanol and NSAID). To elucidate mechanisms of action, the antisecretory action and involvements of NO, SH, mucus and PGE2 were evaluated. The acetic acid-induced gastric ulcer model and Western Blot assay (COX-2 and EGF) were also used to evaluate the OEH healing capacity. GC-MS analysis of OEH indicated three monoterpenes as major compounds: alpha-pinene (50.8%), cineole (20.3%) and beta-pinene (18.3%) and, at the dose of 100 mg/Kg, p.o., OEH provided effective gastroprotection against lesions induced by absolute ethanol (97%) and NSAID (84%) in rats. OEH do not interfere with H+ secretion in gastric mucosa and its gastric protection does not depend on nitric oxide (NO) and sulfhydryl compounds (SH). The gastroprotective action of OEH occurs due to an increase in the gastric mucus production (28%) induced by PGE2 levels. Furthermore, OEH demonstrated a great healing capacity with 87% of reduction in ulcerative lesion area. It accelerated the healing of acetic acid-induced gastric lesions due to an increase in COX-2 (75%) and EGF (115%) expression in gastric mucosa. No sign of toxicity was observed in this study, considering the analyzed parameters. All these results suggest the efficacy and safety of Hyptis spicigera in combating and healing gastric ulcer. Considering the results, it is suggested that the OEH could probably be a good therapeutic agent for

  15. Inhibition of Asthma in OVA Sensitized Mice Model by a Traditional Uygur Herb Nepeta bracteata Benth.

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    Jing Wang

    2016-01-01

    Full Text Available Asthma is a chronic lung inflammation which affects many people. As current therapies for asthma mainly rely on administration of glucocorticoids and have many side effects, new therapy is needed. In this study, we investigated Nepeta bracteata Benth., a traditional Uygur Herb, for its therapeutics effect in OVA induced asthmatic mice model. Treatment of OVA sensitized asthma mice with extract from Nepeta bracteata Benth. demonstrated improved lung pathology, as well as reduced infiltration of eosinophil and neutrophil. Nepeta bracteata Benth. extract also contributed to the rebalance of Th17/Treg cell via decreasing the Th17 cell and increasing the Treg, which was corresponding with the inhibited Th17 cytokine response and increased IL-10 level. Moreover, the reduced TGF-β level and Smad2/3 protein level also suggested that Nepeta bracteata Benth. extract could inhibit TGF-β mediated airway remodelling as well. Taken together, these data suggested that Nepeta bracteata Benth. may be a novel candidate for future antiasthma drug development.

  16. The bioassay-guided isolation of growth inhibitors of adult T-cell leukemia (ATL), from the Jamaican plant Hyptis verticillata, and NMR characterization of hyptoside.

    Science.gov (United States)

    Hamada, Toshiyuki; White, Yohann; Nakashima, Mitsuyoshi; Oiso, Yusuke; Fijita, Masaki J; Okamura, Hiroaki; Iwagawa, Tetsuo; Arima, Naomichi

    2012-08-17

    Through bioassay-guided isolation, five compounds with growth inhibitory activity on S1T, an adult T-cell leukemia (ATL) cell line, were isolated from the crude methanol extract of the aerial parts of Hyptis verticillata.

  17. Enhancement of liver regeneration by the association of Hyptis pectinata with laser therapy.

    Science.gov (United States)

    Melo, Gustavo B; Silva, Renata L; Melo, Valdinaldo A; Lima, Sônia O; Antoniolli, Angelo R; Castro-E-silva, Tiago; Marcassa, Luis G; Bagnato, Vanderlei S; Zucoloto, Sérgio; Ramalho, Leandra N Z; Ramalho, Fernando S; Castro-E-silva, Orlando

    2005-05-01

    Since new molecules that normally would accelerate regeneration can also be potentialized by light, the use of new substances combined with laser therapy seems to be a natural type of experiment. Therefore, the purpose of this study was to assess the effects of Hyptis pectinata leaves on liver regeneration after partial hepatectomy (PH) associated with laser therapy. Twenty-four rats were divided into four groups--PH(control), PHL (laser therapy), PH200 (200 mg/kg of Hyptispectinata), and PHL200 (200 mg/kg of the plant and laser)--which were submitted to 67% hepatectomy. Laser treatment consisted of focusing the light on the remaining liver after hepatectomy. The data analyzed were serum levels of aminotransferases, liver regeneration, and mitochondrial function. Group PH200 showed a statistically significant decrease in AST levels, and PHL200 disclosed an augmentation in ALT levels. The liver regeneration index was significantly increased in group PHL200. Concerning liver mitochondrial respiratory assay, groups PH200 and PHL200 showed lower state 3 levels than groups PH and PHL. Group PHL showed an increase in state 4 levels and a reduction in membrane potential and RCR. The present study shows that the association of the aqueous extract of Hyptis pectinata leaves at 200 mg/kg with intraoperative laser therapy can stimulate liver regeneration and cause a reduction in liver mitochondrial respiratory function without altering its phosphorylative activity.

  18. Anatomia do xilema secundário de Mimosa incana (Spreng. Benth.

    Directory of Open Access Journals (Sweden)

    José Newton Cardoso Marchiori

    2009-09-01

    Full Text Available Normal 0 21 false false false MicrosoftInternetExplorer4 São descritos os caracteres anatômicos do xilema secundário de Mimosa incana (Spreng. Benth. e fornecidas 29 medições de sua estrutura. A descrição é ilustrada com fotomicrografias. A estrutura anatômica indica um alto grau de especialização filogenética e reúne diversos caracteres indicativos da família Leguminosae, sub-família Mimosoideae e série Lepidotae Benth. Normal 0 21 false false false MicrosoftInternetExplorer4

  19. EFEITO DE ALTAS TEMPERATURAS NA GERMINAÇÃO DE SEMENTES DE Plathymenia reticulata Benth. E Dalbergia miscolobium Benth.

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    Daniel de Alencastro Bouchardet

    2015-08-01

    Full Text Available RESUMOO fogo é comum no Cerrado e, geralmente, ocorre durante a seca, quando várias espécies frutificam ou dispersam as sementes. Entretanto, pouco se sabe sobre os efeitos do fogo na germinação das espécies do Cerrado. Este trabalho investigou o efeito da exposição a altas temperaturas na germinação de sementes de Dalbergia miscolobium Benth. e Plathymenia reticulata Benth., comuns no Cerrado e com uso potencial para recuperação de áreas degradadas e na recomposição de áreas de preservação permanente. As sementes foram coletadas em agosto de 2011, pesadas, separadas em três classes de tamanho e expostas a três temperaturas: 80 ºC, 100 ºC e 150 ºC por 2 e 5 minutos. Após os tratamentos, as sementes foram colocadas para germinar. A exposição a altas temperaturas afetou, de forma diferenciada, a germinação das espécies. Independentemente da classe de tamanho, a exposição das sementes até 100 °C, por 2 e 5 minutos, não alterou a germinação de D. miscolobium (91%. Entretanto, apenas as sementes maiores germinaram após a exposição a 150 °C, porém com redução significativa na germinação (53%; p<0,01. Para P. reticulata, a exposição a 80 °C por 2 e 5 minutos e a 100 °C por 2 minutos não alterou a germinação (71%, e nenhuma semente germinou após a exposição a 150 °C. A baixa resistência das sementes às altas temperaturas pode afetar significativamente o recrutamento de novos indivíduos após a ocorrência de queimadas no Cerrado.

  20. Biostimulant on faveiro (Dimorphandra mollis Benth. seeds and seedling vigor

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    Tathiana Elisa Masetto

    2012-06-01

    Full Text Available This work aimed to verify the effects of biostimulant doses, applied through seeds, on faveiro (Dimorphandra mollis Benth. seeds and seedlings vigor. Seeds were previously sulfuric acid scarified in two consecutive years experiments. On 2006 August (first experiment seeds were submitted on Stimulate®: 0; 3.5; 7.0; 10.5 and 14 mL.0.5 Kg-1 doses (first experiment; and seeds picked in August, 2007, were Stimulate:0; 15; 20 and 25 mL.0.5Kg-1 of seeds treated (second experiment. After the biostimulant treatment seeds were sowed in cells trays containing 1:1 (v:v distroferric red latosol + plantmax®. The effect of different Stimulate® doses on Dimorphandra mollis seeds collected in different years were evaluated on the emergency percentage, speed emergency index, root length, aerial part height and dry seedling weight. It was conducted on entirely casualized with four repetitions of 25 seeds each treatment. The 14 mL (2006 lot and 15 mL (2007 lot 0.5Kg-1 of seeds doses provided larger percentage (50% and 66%, respectively and speed emergency indexes (0.67 and 0.9 respectively. The 20 mL.0.5Kg-1 of seeds dose treatment favored the aerial part length, but it didn't influenced the other Dimorphandra mollis seedlings vigor indexes.

  1. Terpenes From the Root of Salvia hypoleuca Benth

    Science.gov (United States)

    2012-01-01

    Background The genus Salvia, with nearly 900 species, is one of the largest members of Lamiaceae family. In the Flora of Iran, the genus Salvia is represented by 58 species of which 17 species are endemic. Salvia hypoleuca Benth., is one of these species growing wildly in northern and central parts of Iran. Salvia species are well known in folk medicine and widely used for therapeutic purposes. Literature review shows that there is no report on phytochemical investigation of the roots of S. hypoleuca. Results The separation and purification process were carried out using various chromatographic methods. Structural elucidation was on the basis of NMR and MS data, in comparison with those reported in the literature. The isolated compounds were identified as sitosteryl oleate (1), β-sitosterol (2), stigmasterol (3), manool (4), 7α-acetoxy royleanone (5), ursolic acid (6), oleanolic acid (7), 3-epicorosolic acid (8), 3-epimaslinic acid (9) and coleonolic acid (10). Conclusions In the present study, three sterols, two diterpenes and five triterpenes were isolated from the ethyl acetate extract of the roots of S. hypoleuca. As the chemotaxonomic significance, some of the isolated compounds (1–7, 9) have not been previously reported from the species S. hypoleuca, while the triterpenes 8 and 10 are now documented from Salvia genus for the first time. PMID:23351362

  2. SINTESIS SENYAWA C18H26O9 DARI HIPTOLIDA HASIL ISOLASI DAUN HYPTIS PECTINATA

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    Meiny Suzery

    2012-05-01

    Full Text Available SYNTHESIS OF C18H26O9 COMPOUNDS FROM HYPTOLIDE ISOLATED FROM HYPTIS PECTINATA LEAVES. Isolation of hyptolide has been done from Hyptis pectinata, and alkene group transformation through oxidation reactions using H3B: OEt2 to the isolated compound was also conducted. Product analyses were carried out using TLC, UV spectrometry, IR, and LC-MS. Pure crystal with melting point of 86-87oC was isolated. The yield was 1.75% (w/w. After analysing and compilating of spectroscopic data it was confirmed as hyptolide compound. Hydroboration of this compound (followed by hydrolysis using H2O2 under alkaline conditions produce its alcohol derivatives, with 28.9% the percentage of transformation, it was demonstrated by LCMS data. IR spectrum at 3600cm-1, confirming the replacement of hydroxyl bond by alkene. Regioselectivity of addition reaction is proposed through simulation with Chem Office. The reaction product was suspected as 6-hydroxy-7-(6-oxo-3,6-dihydro-2H-pyran-2-yl heptane-2,3,5-tryil triacetate. Extension of reaction time to 24 hours, has increase hydroboration product to 78.3%. This research has opened other studies of natural materials in accordance to the roadmap set.  Telah dilakukan isolasi hiptolida dari bahan alam Hyptis pectinata, dan transformasinya melalui reaksi oksidasi menggunakan H3B:OEt2 terhadap gugus alkena pada senyawa hasil isolasi. Analisis produk dilakukan menggunakan KLT, spektrometri UV, IR, dan LC-MS. Kristal murni dengan titik leleh 86-87oC berhasil diisolasi dengan rendemen 1,75 % (b/b, dirujuk sebagai senyawa hiptolida setelah melalui analisis dan kompilasi data-data spektroskopi. Hidroborasi terhadap senyawa hiptolida (yang diikuti hidrolisis menggunakan H2O2 dalam suasana basa menghasilkan senyawa alkohol turunannya, dengan persentase transformasi sebesar 28,9%, dapat ditunjukkan melalui data LCMS. Data spectrum IR menunjukkan adanya puncak pada 3600cm-1, memperkuat dugaan  adanya ikatan hidroksil menggantikan gugus

  3. Would Sacaca, Croton cajucara Benth (Euphorbiaceae be an hepatotoxic plant like Germander, Teucrium chamaedrys L. (Labiatae?

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    Manoel do Carmo Pereira Soares

    Full Text Available Clinical and experimental studies have consistently incriminated the medicinal plant germander (Teucrium chamaedrys L. in epidemic and sporadic cases of liver diseases. The sacaca (Croton cajucara Benth, a common plant in Brazilian Amazon region also comes being incriminated in similar clinical cases. Of both plants were isolated diterpenoid coumpounds with similar chemical structures.

  4. Would Sacaca, Croton cajucara Benth (Euphorbiaceae) be an hepatotoxic plant like Germander, Teucrium chamaedrys L. (Labiatae)?

    Science.gov (United States)

    Soares, Manoel do Carmo Pereira

    2004-01-01

    Clinical and experimental studies have consistently incriminated the medicinal plant germander (Teucrium chamaedrys L.) in epidemic and sporadic cases of liver diseases. The sacaca (Croton cajucara Benth), a common plant in Brazilian Amazon region also comes being incriminated in similar clinical cases. Of both plants were isolated diterpenoid compounds with similar chemical structures.

  5. Would Sacaca, Croton cajucara Benth (Euphorbiaceae) be an hepatotoxic plant like Germander, Teucrium chamaedrys L. (Labiatae)?

    OpenAIRE

    Soares,Manoel do Carmo Pereira

    2004-01-01

    Clinical and experimental studies have consistently incriminated the medicinal plant germander (Teucrium chamaedrys L.) in epidemic and sporadic cases of liver diseases. The sacaca (Croton cajucara Benth), a common plant in Brazilian Amazon region also comes being incriminated in similar clinical cases. Of both plants were isolated diterpenoid coumpounds with similar chemical structures.

  6. New adduct of abietane-type diterpene from Salvia leriifolia Benth.

    Science.gov (United States)

    Hussain, Amjad; Adhikari, Achyut; Iqbal Choudhary, M; Ayatollahi, Syed Abdulmajid; Atta-Ur-Rahman

    2016-07-01

    A new adduct of abietane-type diterpene, salvialeriicone (1), was isolated from Salvia leriifolia Benth., along with a new chemical entity nor-abietane diterpene, 2-isopropyl-8,8-dimethyl-7,8-dihydrophenanthrene-1,4,5(6H)-trione (2). Their structures were determined using mass spectrometry, and 1D- and 2D-NMR spectroscopy.

  7. Allelopathic potential of Hyptis suaveolens on physio-biochemical changes of mung bean seeds

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    Parthapratim Maiti

    2015-10-01

    Full Text Available Hyptis suaveolens is an exotic invasive weed in many areas of West Bengal, India. The allelopathic potential of leaf extracts and leachates of H. suaveolens was investigated on germination and metabolism of mung bean seeds (Vigna radiata cv. K851. The extracts and leachates reduced the germination and seed viability. The insoluble carbohydrates, proteins, and the activities of dehydrogenase and catalase enzymes were significantly reduced. Amino acid and soluble carbohydrate levels were increased in seeds pretreated with leaf extracts and leachates. The overall biochemical results indicate that various inhibitors present in H. suaveolens impart strong inhibitory effect on mung bean. The leaves of H. suaveolens possess phytotoxic chemicals, which potentially rendered the inhibitory action on mung bean seeds and provided key information for the proper management of H. suaveolens and other invasive weeds showing similar behavior.

  8. Effect of the aqueous extract of Hyptis pectinata on liver mitochondrial respiration.

    Science.gov (United States)

    Melo, G B; Silva, R L; Melo, V A; Antoniolli, A R; Souza, M E Jordani; Jordani, M C; Castro-e-Silva, O

    2005-05-01

    Some studies have indicated that mitochondria may be the target organelle of plants. We therefore decided to assess the effects of the aqueous extract of Hyptis pectinata leaves on liver mitochondrial respiratory function in vitro. Eight rat livers were subjected to isolation of mitochondria by differential centrifugation. In an adequate medium, the plant extract was added at different concentrations. The analyzed data were: state 3, state 4 and respiratory control ratio (RCR). H. pectinata extract caused a statistically significant decrease in state 3 (at 0.05, 0.1 and 0.2 mg/mg protein) and RCR (at 0.05, 0.1 and 0.2 mg/mg protein). Respiratory state 4 was not altered by the increasing concentrations. In conclusion, the aqueous extract of H. pectinata leaves may not injure the mitochondrial inner membrane but decreases significantly the oxidative phosphorylation.

  9. Prebiotic potential of neutral oligo- and polysaccharides from seed mucilage of Hyptis suaveolens.

    Science.gov (United States)

    Mueller, Monika; Čavarkapa, Andrea; Unger, Frank M; Viernstein, Helmut; Praznik, Werner

    2017-04-15

    Prebiotics are selectively fermented by the gastrointestinal microflora, resulting in benefits to human health. The seed mucilage of Hyptis suaveolens contains neutral and acidic polysaccharides in a ratio of 1:1. The neutral polysaccharides consist of galactose, glucose and mannose whereas the acidic polysaccharides contain fucose, xylose and 4-O-methylglucuronic acid -residues. The growth of probiotics in the presence of total, acidic or neutral polysaccharides and oligosaccharides was tested using turbidity measurements. The majority (11 out of 14) of the tested probiotic strains significantly grew in the neutral fraction. Growth occurred with some time delay, but may be longer lasting than with other lower molecular prebiotics. The extent of growth increased with neutral polysaccharides from H. suaveolens corresponding to the externally available galactose units (20%). In conclusion, neutral poly- and oligosaccharides from H. suaveolens have a prebiotic potential characterized by a delayed but long lasting effect. Copyright © 2016 Elsevier Ltd. All rights reserved.

  10. Antinociceptive activity of the volatile oils of Hyptis pectinata L. Poit. (Lamiaceae) genotypes.

    Science.gov (United States)

    Arrigoni-Blank, M F; Antoniolli, A R; Caetano, L C; Campos, D A; Blank, A F; Alves, P B

    2008-05-01

    Hyptis pectinata L. Poit (Lamiaceae) is known popularly in Brazil as "sambacaita" or "canudinho" and is used in the treatment of inflammations, bacterial infections and ache. The antinociceptive activity of the volatile oils of six genotypes, at doses of 100, 200 and 400mg/kg body wt., were investigated using abdominal writhe models induced by acetic acid and hot plate tests. The volatile oils of all the genotypes are composed mainly of sesquiterpenoids. All the genotypes showed antinociceptive effects in both models used; the SAM002 genotype showed the major inhibitory effect at dose of 100mg/kg body wt. These results suggest that the volatile oil of H. pectinata has peripheral (writhe reduction) and central (time delay of thermal reaction) effects. These observations indicate that H. pectinata may be useful as an analgesic drug.

  11. COMPRIMENTO DE ESTACAS NA PROPAGAÇÃO VEGETATIVA DE BAMBURRAL (Hyptis suaveolens (L. POIT.

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    ANA CLÁUDIA DA SILVA

    2011-01-01

    Full Text Available Hyptis suaveolens (L. Poit. (Lamiaceae is a medicinal plant widely used in folk medicine in Northeast Brazil as a treatment for respiratory diseases, antispasmodic, sweat and useful in the treatment of gout. The aim of this study was to evaluate hereto the influence of different lengths of cuttings in rooting ofbamburral. The test was conducted for 30 days in the greenhouse of the Department of Environmental Sciences, Federal University of Semi-Arid (UFERSA. The experimental design was randomized blocks with four cutting lengths (5; 10; 15 and 20 cm and four replications and 10 cuttings per plot. After 30 days of planting, characteristics were evaluated: percentage of survival, rooting percentage, longest root length, diameter and initial end of cutting, the dry mass of shoot and root. Conclude that the length of piles does not affect the rooting of bamburral.

  12. Effects of the aqueous extract from Hyptis pectinata leaves on rodent central nervous system

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    Alexsandro X. Bueno

    Full Text Available The effects of the aqueous extract (AE from Hyptis pectinata leaves was studied on rodent central nervous system (CNS. Pharmacological screening, open field, forced swimming, apomorphine-induced hypothermia, elevated plus maze and thiopental-induced sleep tests were used in male and female Swiss mice and Wistar rats. The AE was admnistered orally in single doses 30 minutes before each test. In the screening test the AE (400 mg/kg, p.o., n =3 decreased the frequency of grooming behavior. In the open field test it decreased the amount of time rats spent grooming (AE400: 51.3 ± 8.8, q = 5.513, P < 0.01, n = 10, similarly to diazepam (control: 107.3 ± 14.2; diazepam: 15.4 ± 4.3, q = 9.049, P < 0.001, n = 10. In the forced swimming test the AE (400 mg/kg decreased the immobility time (con: 181.3 ± 7.2 s; imip: 91.6 ± 8.9 s, q = 7.958, P < 0.001; AE400: 111.6 ± 14.5 s, q = 6.193, P < 0.001, n = 9, indicating a possible antidepressive effect. This was confirmed in the apomorphine-induced hypothermia model where the extract (200 mg/kg antagonized the apomorphine effect (AE200: -0.27 ºC, q = 5.588, P < 0.001, n = 10, similarly to imipramine (imipramine: 5 mg/kg (control: -1.08 ºC; imipramine: 0.02 ºC, q = 7.589, P < 0.001, n = 10. No effect of the AE was detected in the elevated plus maze and in the sleeping time induced by thiopental models. Our results suggest a possible antidepressive effect of the aqueous extract obtained from the leaves of Hyptis pectinata.

  13. Proliferative effect of the aqueous extract of Hyptis pectinata on liver regeneration after partial hepatectomy in rats.

    Science.gov (United States)

    Melo, Gustavo Barreto; Silva, Renata Lemos; Melo, Valdinaldo Aragão; Antoniolli, Angelo Roberto; Michellone, Paulo Roberto Teixeira; Zucoloto, Sérgio; Souza, Maria Eliza Jordani de; Gomes, Maria Cecília Jordani; Correia, Rodrigo Borges; Silva, Orlando de Castro e

    2006-01-01

    This study was carried out to assess the effects of the aqueous extract of Hyptis pectinata leaves on liver regeneration and on serum enzymes (AST, ALT and gamma-GT) after 67% partial hepatectomy in rats. AST, ALT and gamma-GT, were determined by conventional procedures using a spectrophotometer (Model E2250-CELM). Liver regeneration was evaluated by immunohistochemical staining for proliferating cell nuclear antigen (PCNA). Oral pretreatment during 4 days at 100 mg/kg increased liver regeneration index. At 200 mg/kg, AST level was statistically decreased in comparison to the group submitted to distilled water administration. The other enzymes assessed disclosed no difference when all groups were compared. The present study shows that the aqueous extract of Hyptis pectinata leaves contains some biological active principles that stimulate liver regeneration at 100 mg/kg and cause slight hepatic protection at 200 mg/kg.

  14. Anti-hyperglycemic activity of leaves extract of Hyptis suaveolens L. Poit in streptozotocin induced diabetic rats.

    Science.gov (United States)

    Mishra, Shanti Bhushan; Verma, Amita; Mukerjee, Alok; Vijayakumar, M

    2011-09-01

    To evaluate the antihyperglycemic activity of leaves of Hyptis suaveolens using streptozotocin model. Hyptis suaveolens extract (HSE) 250 and 500 mg/kg body weight was administered orally to streptozotocin induced diabetes, once daily for 21 days. A significant reduction in blood glucose was observed in diabetic animals treated with HSE at different doses when compared with diabetic rats. Levels of triglyceride, total cholesterol, low density lipoprotein, very low density lipoprotein were decreased while administering HSE at different doses, compared with their control values in diabetic animals. Our results show that HSE possesses significant antihyperglycemic activity which might be attributed to stimulating effects on glucose utilization and antioxidant enzyme. Copyright © 2011 Hainan Medical College. Published by Elsevier B.V. All rights reserved.

  15. Influence of storage time and temperature on the chemical composition of the essential oil of Hyptis pectinata L. Poit

    OpenAIRE

    JESUS,A.S.; BLANK,A.F.; ALVES,M.F.; ARRIGONI-BLANK,M.F.; LIMA,R.N.; ALVES,P.B.

    2016-01-01

    ABSTRACT Hyptis pectinata, popularly known as “sambacaitá”, is a native medicinal herb used by local people for treating disease. Its pharmacological, antimicrobial, and medicinal properties have been reported in several studies. The essential oil of the leaves is rich in terpene compounds responsible for its properties. Because there are factors that can influence the chemical composition of essential oils, the objective of this study was to evaluate the influence of storage on H...

  16. Mosquito Repellent Activity and Phytochemical Characterization of Essential Oils From Striga hermonthica, Hyptis spicigera and Ocimum basilicum Leaf Extracts

    OpenAIRE

    Gabi Baba; A.O. Lawal; Hauwa B. Sharif

    2012-01-01

    The main aim of this study is to screen the phytochemicals and compare the mosquito repellent activities of essential oils from Hyptis spicigera, Striga hermonthica and Ocimum basilicum (Basil) against Anopheles gambiae and Culex quinquefasciatus under laboratory conditions. The global threat of malaria to human race and the need to control its advances is on the focus. Mosquito is the target being the primary host in the spread of malaria. Alkaloids, saponnins, steroids, tannins and terpenoi...

  17. The anti-Helicobacter pylori flavones in a Brazilian plant, Hyptis fasciculata, and the activity of methoxyflavones.

    Science.gov (United States)

    Isobe, Takahiko; Doe, Matsumi; Morimoto, Yoshiki; Nagata, Kumiko; Ohsaki, Ayumi

    2006-05-01

    Two known flavonoids were isolated from a tropical medicinal plant, Hyptis fasciculata (Labiatae), found in Brazil. Flavonoids were identified as cirsilineol (1) and cirsimaritin (2) by spectroscopic means and were exhibited potent antibacterial activity against Helicobacter pylori, and cirsilineol (1) had weak antibacterial activity against Escherichia coli and Salmonella enteritidis. Following up on the relationship between anti-H. pylori activity and flavonoids with methoxy groups, several methoxy flavonoids were evaluated for proliferation of H. pylori.

  18. GC-MS and FT-IR analysis of a coastal medicinal plant-Hyptis suaveolens (L.) Poit

    OpenAIRE

    Joselin Joseph; Solomon Jeeva

    2016-01-01

    Objective: To investigate the bioactive components of a coastal medicinal plant, Hyptis suaveolens (L.) Poit. (H. suaveolens) leaves using fourier transform-infrared spectroscopy and gas chromatography-mass spectrometer (GC-MS). Methods: The chemical compositions of the ethanol extract of whole plant of H. suaveolens was investigated using PerkinElmer GC-MS, while the mass spectra of the compounds found in the extract was matched with the National Institute of Standard and Tech...

  19. Efeito do extrato aquoso da Hyptis pectinata sobre a proliferação de hepatócitos após hepatectomia parcial Effect of the aqueous extract of Hyptis pectinata on hepatocyte proliferation after partial hepatectomy

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    Renata Lemos Silva

    2002-01-01

    Full Text Available OBJETIVO: O uso de plantas medicinais tem aumentado mundialmente. Então, é necessário saber seus possíveis efeitos a fim de estabelecer seu uso correto em seres humanos. Dessa forma, neste trabalho se propôs a estudar os efeitos do extrato aquoso das folhas da Hyptis pectinata, popularmente conhecida como "sambacaitá" ou "canudinho", sobre a regeneração hepática após hepatectomia parcial em ratos. MÉTODOS: Dez ratos foram divididos em 2 grupos: grupos HP, que foi submetido à hepatectomia de 67% após 4 dias de administração de água destilada; e o grupo HP100, que foi submetido à hepatectomia parcial e administração oral de 100 mg/kg do extrato aquoso da Hyptis pectinata pelo mesmo período de tempo. Todos os animais foram sacrificados 24 horas após o procedimento cirúrgico. A regeneração foi analisada através do método de imuno-histoquímica PCNA, usando o anticorpo monoclonal PC-10. RESULTADOS: O índice de regeneração hepática estava significativamente elevado no grupo HP100 (pOBJECTIVE: The use of medicinal plants has increased worldwide. Therefore, it is necessary to know their possible effects in order to establish their correct use on human beings. Taking it into account, the objective of this study was to assess the effects of the aqueous extract of Hyptis pectinata leaves, popularly known as "sambacaitá" or "canudinho", on liver regeneration after partial hepatectomy in rats. METHODS: Ten rats were divided into 2 groups: HP group, which underwent 67% hepatectomy after 4 days of distilled water administration; and HP100 group, which was submitted to partial hepatectomy and oral administration of 100 mg/kg of the aqueous extract of Hyptis pectinata for the same period of time. All animals were sacrificed 24 hours after the surgical procedure. Liver regeneration was evaluated by immunohistochemical staining for proliferating cell nuclear antigen (PCNA using the PC-10 monoclonal antibody. RESULTS: The hepatic

  20. El género Macroptilium (Benth. Urb. (Leguminosae en Cuba

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    Beyra Matos, Ángela

    2005-12-01

    Full Text Available We present a preliminary synopsis of the Cuban species of Macroptilium (Benth. Urb. that includes keys, descriptions, illustrations, chromosome numbers, palinology, phenology, ecology, distribution maps, notes on chemical composition and ethnobotany for each of the four species known for Cuba. We also discuss on the diagnostic characters of the genus and the species present in the Cuban flora.Se presenta una sinopsis de las especies del género Macroptilium (Benth. Urb. presentes en Cuba. Se incluyen claves dicotómicas, descripciones, ilustraciones, datos químicos, cromosómicos, palinológicos, fenológicos, etnobotánicos, así como la distribución y ecología de las cuatro especies del género. Se discuten los caracteres diagnósticos del género y de las especies presentes en Cuba.

  1. Volatile secondary metabolites of Micromeria dalmatica Benth. (Lamiaceae): biosynthetical and chemotaxonomical aspects.

    Science.gov (United States)

    Radulović, Niko S; Blagojević, Polina D

    2012-07-01

    Analysis by GC and GC/MS of the essential oil obtained from above-ground parts of Micromeria dalmatica Benth. allowed the identification of 116 components, comprising 93.6% of the total oil composition. The major compounds are 3-oxygenated p-menthane monoterpenes and were identified as pulegone (29.6%), menthone (11.7%), and piperitenone (10.8%). The chemical composition of this and additional 30 oils obtained from selected Micromeria Benth. taxa were compared by using multivariate statistical analysis (agglomerative hierarchical cluster analysis and principal component analysis (PCA)). The results of statistical analyses, as well as the domination of different concurrent p-menthane-skeleton-type monoterpene biosynthetical sub-branches in the compared M. dalmatica samples, implied the occurrence of at least two different chemotypes of the mentioned species. Copyright © 2012 Verlag Helvetica Chimica Acta AG, Zürich.

  2. New species of Hymenoptera associated with galls on Calliandra brevipes Benth. (Fabaceae, Mimosoidea) in Brazil

    OpenAIRE

    Angélica M. Penteado-Dias; Carvalho, Fabrício M. de

    2008-01-01

    Four species of Hymenoptera: Tanaostigmodes ringueleti (Brèthes, 1924), T. mecanga sp.nov. (Chalcidoidea, Tanaostigmatidae), Allorhogas taua sp. nov. (Braconidae, Doryctinae) and Eurytoma sp. (Chalcidoidea, Eurytomidae) were reared from two different types of galls of Calliandra brevipes Benth. (Fabaceae, Mimosoidea) in Juiz-de-Fora, Minas Gerais State, Brazil. The two Tanaostigmatidae species are probably the gall inducers; the Braconidae species probably is phytophagous inquiline in round g...

  3. Cytotoxic sesquiterpene lactones from the leaves of Vernonia guineensis Benth. (Asteraceae)

    Science.gov (United States)

    Toyang, Ngeh J.; Wabo, Hippolyte K.; Ateh, Eugene N.; Davis, Harry; Tane, Pierre; Sondengam, Luc B.; Bryant, Joseph; Verpoorte, Rob

    2015-01-01

    Ethnopharmacological relevance Vernonia guineensis Benth. (Asteraceae) preparations are used in folk medicine in Cameroon to treat a number of ailments, including prostate cancer and malaria, and is used as an anthelmintic, adaptogen and antidote. The aim of this study was to continue the validation of the activity of Vernonia guineensis Benth. extracts and isolated molecules against cancer cell lines following the previous isolation of an anti-prostate cancer sugar ester from the root extract. Materials and methods Acetone extracts of Vernonia guineensis Benth. leaves were tested for activity against 10 cancer cell lines (Breast—MDA-MB-231, Breast—MCF-7, Colon—HCT-116, Leukemia—HL-60, Lung—A549, Melanoma—A375, Ovarian—OVCAR3, Pancreas—Mia-paca, Prostate—PC-3 and Prostate—DU-145). The acetone extract was subjected to bioactivity guided fractionation. Anti-proliferation and clonogenic activity of the isolated compounds were tested. The WST-1 assay was used for the anti-proliferation activity, while the standard clonogenic test was used to determine the clonogenic activity. Results The acetone extract of Vernonia guineensis Benth. demonstrated in vitro activity ranging from IC50 4–26 mg/mL against the 10 cell lines. Activity guided fractionation of this extract yielded two sesquiterpene lactones, isolated for the first time from the genus Vernonia. The compounds were characterized using spectroscopic experiments, including a combination of 1D and 2D NMR data. Vernopicrin (1) and Vernomelitensin (2) demonstrated in vitro activity against human cancer cell lines with IC50 ranging from 0.35–2.04 μM (P sesquiterpenes against cancer cell lines highlights the medicinal potential of V. guineensis. The selective anti-proliferation and dose dependent anticlonogenic activities suggest that the identified sesquiterpenes could be potential antitumor agents.. PMID:23376285

  4. Phytochemical study of essential oil from the aerial parts of Coleus aromaticus Benth.

    Science.gov (United States)

    Tewari, G; Pande, C; Kharkwal, G; Singh, S; Singh, C

    2012-01-01

    The essential oil composition of Coleus aromaticus Benth. (family Lamiaceae) was examined by capillary GC and GC-MS. Analyses revealed the presence of 28 constituents, of which 16 were identified. Thymol (83.39%) was found to be the major compound, while 1-octen-3-ol, terpine-4-ol, eugenol, trans-caryophyllene, caryophyllene oxide and α-cadinol were present as minor constituents.

  5. Cytotoxic sesquiterpene lactones from the leaves of Vernonia guineensis Benth. (Asteraceae).

    Science.gov (United States)

    Toyang, Ngeh J; Wabo, Hippolyte K; Ateh, Eugene N; Davis, Harry; Tane, Pierre; Sondengam, Luc B; Bryant, Joseph; Verpoorte, Rob

    2013-03-27

    Vernonia guineensis Benth. (Asteraceae) preparations are used in folk medicine in Cameroon to treat a number of ailments, including prostate cancer and malaria, and is used as an anthelmintic, adaptogen and antidote. The aim of this study was to continue the validation of the activity of Vernonia guineensis Benth. extracts and isolated molecules against cancer cell lines following the previous isolation of an anti-prostate cancer sugar ester from the root extract. Acetone extracts of Vernonia guineensis Benth. leaves were tested for activity against 10 cancer cell lines (Breast-MDA-MB-231, Breast-MCF-7, Colon-HCT-116, Leukemia-HL-60, Lung-A549, Melanoma-A375, Ovarian-OVCAR3, Pancreas-Mia-paca, Prostate-PC-3 and Prostate-DU-145). The acetone extract was subjected to bioactivity guided fractionation. Anti-proliferation and clonogenic activity of the isolated compounds were tested. The WST-1 assay was used for the anti-proliferation activity, while the standard clonogenic test was used to determine the clonogenic activity. The acetone extract of Vernonia guineensis Benth. demonstrated in vitro activity ranging from IC50 4-26μg/mL against the 10 cell lines. Activity guided fractionation of this extract yielded two sesquiterpene lactones, isolated for the first time from the genus Vernonia. The compounds were characterized using spectroscopic experiments, including a combination of 1D and 2D NMR data. Vernopicrin (1) and Vernomelitensin (2) demonstrated in vitro activity against human cancer cell lines with IC50 ranging from 0.35-2.04μM (Psesquiterpenes against cancer cell lines highlights the medicinal potential of V. guineensis. The selective anti-proliferation and dose dependent anticlonogenic activities suggest that the identified sesquiterpenes could be potential antitumor agents. Copyright © 2013 Elsevier Ireland Ltd. All rights reserved.

  6. Would Sacaca, Croton cajucara Benth (Euphorbiaceae) be an hepatotoxic plant like Germander, Teucrium chamaedrys L. (Labiatae)?

    OpenAIRE

    Soares, Manoel do Carmo Pereira

    2004-01-01

    Clinical and experimental studies have consistently incriminated the medicinal plant germander (Teucrium chamaedrys L.) in epidemic and sporadic cases of liver diseases. The sacaca (Croton cajucara Benth), a common plant in Brazilian Amazon region also comes being incriminated in similar clinical cases. Of both plants were isolated diterpenoid coumpounds with similar chemical structures. Estudos clínicos e experimentais tem incriminado, de forma consistente, na França, a planta medicinal g...

  7. Post-harvest Treatment of the Pogostemon cablin Benth Oil Yield

    OpenAIRE

    TASKIRAWATI, Ira

    2016-01-01

    Patchouli oil is one of the essential oils that are important in the perfume industry, cosmetics, and medicine. Patchouli oil is obtained from the distillation of the trunk, branches, and leaves of patchouli. One of the factors affecting the yield of patchouli oil is a post-harvest treatment. This study aims to determine the yield of patchouli (Pogostemon cablin Benth) oil from different processes in post-harvest management. There were two post-harvest treatments committed to patchouli leaves...

  8. Evaluation ofin vitro antiviral activity ofVitex Negundo L., Hyptis suaveolens (L poit., Decalepis hamiltonii Wight & Arn., to Chikungunya virus

    Directory of Open Access Journals (Sweden)

    Sangeetha Kothandan

    2014-02-01

    Full Text Available Objective: To screen the three Indian plants for the antiviral activity to chikungunya virus since chikungunya infections are treated symptomatically without specific drugs till date. Methods: In vitro cytotoxicity assay of the lyophilised extracts was assessed in vero cells for the determination of maximum non toxic concentration and in vitro antiviral assay was evaluated by the inhibition of virus induced cytopathic effect. Results: Aqueous and aqueous ethanolic extracts of Hyptis suaveolens exhibited partial inhibition to Asian strain of chikungunya virus. Conclusion: Of all the three plants tested for antiviral activity to both the lineages of chikungunya virus, Hyptis suaveolens were found to be effective to Asian strain of chikungunya virus.

  9. Estudo anatômico do xilema secundário de Mimosa trachycarpa Benth Anatomy of the secondary xylem of Mimosa trachycarpa Benth

    Directory of Open Access Journals (Sweden)

    José Newton Cardoso Marchiori

    1997-06-01

    Full Text Available São descritos os caracteres microscópicos da madeira de Mimosa trachycarpa Benth., fornecidos dados quantitativos, determinações estereológicas e fotomicrografias de sua estrutura anatômica. Foram encontrados raios muito finos, parênquima paratraqueal, fibras libriformes não septadas, pontoações ornamentadas e poros agrupados em cachos, com arranjo tendente a diagonal. A estrutura da madeira é comparada com referências da literatura para a família Leguminosae e gênero Mimosa, em análise filogenética, taxonômica e ecológica.The wood anatomy of Mimosa trachycarpa Benth. is described. Photomicrographs as well as quantitative and stereological data of its minute structure were furnished. Very fine rays, paratracheal parenchyma, libriform and not septated fibres, vestured pits and pores in clusters, tending to a diagonal pattern, were found. The wood anatomy was checked with literature of the Leguminosae family and genus Mimosa, considering its phylogenetical, taxonomical and ecological aspects.

  10. Chemical composition and antifungal activity of Hyptis suaveolens (L. poit leaves essential oil against Aspergillus species

    Directory of Open Access Journals (Sweden)

    Ana Carolina Pessoa Moreira

    2010-03-01

    Full Text Available This study aimed to identify the constituents of the essential oil from Hyptis suaveolens (L. leaves using a Gas Chromatograph -Mass Spectrometer and assess its inhibitory effect on some potentially pathogenic Aspergilli (A. flavus, A. parasiticus, A. ochraceus, A. fumigatus and A. niger. Eucaliptol (47.64 % was the most abundant component in the oil, followed for gama-ellemene (8.15 %, beta-pynene (6.55 %, (+3-carene (5.16 %, trans-beta-cariophyllene (4.69 % and germacrene (4.86 %. The essential oil revealed an interesting anti-Aspergillus property characterized by a Minimum Inhibitory Concentration and Minimum Fungicidal Concentration of 40 and 80 µL/mL, respectively. The oil at 80 and 40 µL/mL strongly inhibited the mycelial growth of A. fumigatus and A. parasiticus along 14 days. In addition, at 10 and 20 µL/mL the oil was able to cause morphological changes in A. flavus as decreased conidiation, leakage of cytoplasm, loss of pigmentation and disrupted cell structure suggesting fungal wall degeneration. These findings showed the interesting anti-Aspergillus property of H. suaveolens leaves essential oil supporting its possible rational use as alternative source of new antifungal compounds to be applied in the aspergillosis treatment.

  11. Study of antinociceptive effects on acute pain treated by bioactive fractions of Hyptis suaveolens

    Directory of Open Access Journals (Sweden)

    Azmathunnisa Begum

    2016-09-01

    Full Text Available Objective: To investigate the ethanolic extract and its fractions of Hyptis suaveolens (H. suaveolens for antinociceptive and central nervous system depressant effects. Methods: Dried and coarsely powdered aerial parts of plant material were extracted in 80% aqueous ethanol. Further extract was fractionated using solvents of varying polarity. Analgesic properties was assessed using acetic acid-induced writhing and hot plate test and locomotor activity were performed in mice using hole board test. Results: The petroleum ether and ethyl acetate extracts had produced significant analgesic properties and were found to be maximum when tested at 400 mg/kg. Both extracts significantly increased the latency time in hot plate test and the action was antagonised by naloxone. The naloxone was not able to alter H. suaveolens induced antinociceptive effect in writhing test. Conclusions: From the point of central nervous system depressant and good protective effect on chemical and thermal pain stimuli, it indicates that H. suaveolens might have resulted from activation of opioid and/or peripheral receptors.

  12. Chemical composition and biological activities of essential oil from Hyptis crenata growing in the Brazilian cerrado.

    Science.gov (United States)

    Violante, Ivana Maria Póvoa; Garcez, Walmir Silva; Barbosa, Carolina da Silva; Garcez, Fernanda Rodrigues

    2012-10-01

    Essential oils from species of the genus Hyptis are well-known for their significant biological properties, including antimicrobial and acaricidal activities. The essential oil from the aerial parts of H. crenata was obtained by hydrodistillation; bomeol (17.8%), 1,8-cineol (15.6%) and p-cimene (7.9%) were characterized by GC-MS as its major constituents. The essential oil was evaluated in vitro for its antimicrobial activities against six fungal and five bacterial strains, by measuring the respective MICs, MFCs and MBCs, using broth microdilution methods. The strongest bactericidal activities were shown against Staphylococcus aureus and Enterococcus faecalis, while the strongest fungicidal activities were against Cryptococcus neoformans, Candida glabrata and Candida tropicalis. The oil was also assessed for its anti-tick properties and, at a concentration of 2.5%, it significantly inhibited in vivo oviposition of engorged females of the cattle tick Rhipicephalus (Boophilus) microplus, using the adult immersion test., with an effectiveness of 94.4%.

  13. Purification and biochemical characterization of 11S globulin from chan (Hyptis suaveolens L. Poit) seeds.

    Science.gov (United States)

    Bojórquez-Velázquez, Esaú; Lino-López, Gisela J; Huerta-Ocampo, José A; Barrera-Pacheco, Alberto; Barba de la Rosa, Ana P; Moreno, Abel; Mancilla-Margalli, N Alejandra; Osuna-Castro, Juan A

    2016-02-01

    Chan (Hyptis suaveolens) is a Mesoamerican crop highly appreciated since the pre-Hispanic cultures. Its proteins are a good source of essential amino acids; however, there are no reports on the properties of its individual proteins. In this study, the 11S globulin (Hs11S) was purified and biochemically characterized. The molecular weight of native Hs11S was about 150-300 kDa with isoelectric points of 5.0-5.3, composed by four monomers of 53.5, 52, 51.1 and 49.5 kDa, each formed by one acidic subunit and one basic subunit linked by a disulfide bond. Dynamic light scattering, size exclusion chromatography and native PAGE show that Hs11S is assembled in different oligomeric forms. LC-MS/MS analysis confirmed its identity. Hs11S presents antigenic determinants in common with lupin 11S globulin. Carbohydrate moieties or phosphate groups linked to Hs11S were not detected. This information is very useful in order to exploit and utilize rationally chan 11S globulin in food systems. Copyright © 2015 Elsevier Ltd. All rights reserved.

  14. Novel cytotoxic isolated from Jamaican Hyptis verticillata jacq induces apoptosis and overcomes multidrug resistance.

    Science.gov (United States)

    White, Yohann; Hamada, Toshiyuki; Yoshimitsu, Makoto; Nakashima, Mitsuyoshi; Hachiman, Miho; Kozako, Tomohiro; Matsushita, Kakushi; Uozumi, Kimiharu; Suzuki, Shinsuke; Kofune, Hiroki; Furukawa, Tatsuhiko; Arima, Naomichi

    2011-12-01

    Adult T-cell leukemia is an aggressive hematological malignancy with a poor clinical prognosis, and a rapid resistance to chemotherapy is rapid. Cytotoxicity assay-directed fractionation identified a novel lignan-related agent, 4-methoxy-9-(3,4,5-trimethoxyphenyl)-8, 9 - dihydrofuro[3',4':6,7]naphtho[2,3-d][1,3]dioxol-6(5H)-one (4-MTDND) from the Jamaican plant Hyptis verticillata jacq, and its effects on apoptosis, cell cycle and drug resistance were elucidated. The novel agent, 4-MTDND, exhibited cytotoxicity against myriad cancer types, with a wide therapeutic index of 30- to 40-fold, promoted G(2)/M arrest and up-regulated expression of pro-apoptotic proteins p53 and BAX, as well as enhanced activation of caspase-3, caspase-9 and poly (ADP ribose) polymerase, consistent with apoptosis induction. Multidrug-resistant cancer cells were as susceptible to 4-MTDND as their non-resistant control counterparts, with 4-MTDND having greater efficacy compared to standard chemotherapy agents etoposide and mitoxantrone. The novel cytotoxic agent 4-MTDND induces G(2)/M arrest and apoptosis in cancer cells possibly due to direct DNA damage or interference with topoisomerase II.

  15. Temperature and water relations for sun and shade leaves of a desert broadleaf, Hyptis emoryi

    Energy Technology Data Exchange (ETDEWEB)

    Smith, W.K.; Nobel, P.S.

    1977-02-01

    The temperature and water relations of sun versus shade leaves of Hyptis emoryi Torr. were evaluated from field measurements made in late summer. Throughout most of the day sun leaves had higher temperatures and higher resistances to water vapor diffusion, but lower transpiration rates and lower stem water potentials, than did shade leaves. Leaf absorptivity to solar irradiation was less for 1.5-cm-long sun leaves (0.44) than for 4.0-cm shade leaves (0.56). For both leaf types the stomatal resistance increased as the water vapor concentration drop from the leaf to the air increased. Energy balance equations were used together with the measured temperature dependence of photosynthesis to predict the effect of variations in leaf absorptivity, length, and resistance on net photosynthesis. The influence of leaf dimorphism on whole plants was determined by calculating daily photosynthesis and transpiration for plants with various percentages of sun and shade leaves. A hypothetical plant with all sun leaves in the sun had about twice the photosynthesis and half the transpiration ratio as did plants with sun leaves in the shade or shade leaves in the sun or shade. Plants with both sun and shade leaves had the highest predicted photosynthesis per unit ground area. The possible adaptive significance of the seasonal variation in sun and shade leaf percentages observed for individual H. emoryi bushes is discussed in terms of water economy and photosynthesis.

  16. Analysis of protein fractions and some minerals present in chan (Hyptis suaveolens L.) seeds.

    Science.gov (United States)

    Aguirre, Cesar; Torres, Iovanna; Mendoza-Hernández, Guillermo; Garcia-Gasca, Teresa; Blanco-Labra, Alejandro

    2012-01-01

    Chan (Hyptis suaveolens L.) seeds have been used as food as well as in traditional medicine in several countries of America, Asia and Africa. Chan seed protein content was 13.9% on dry weight basis. Analysis of its protein composition showed 39% globulins, 36% glutelins, 24% albumins, and 1% prolamins. By defatting the flour with chloroform/methanol, it increased the extracted proteins and improved the protein band resolution after SDS-PAGE, showing 5 albumin bands, 8 globulin bands, and 2 prolamin and glutelin bands. The aromatic amino acid content in chan seeds is higher than those of other grains including maize, with good levels of branched chain amino acids. In general, except for lysine, it has a well-balanced amino acid composition, providing a good supply of almost all the essential amino acids for the different age groups. Magnesium content was high, whereas calcium, potassium, and phosphorous were in the average range when compared to barley, oat, rice, and wheat. The present results indicate that seeds from the chan plant could be relevant because of their nutritional properties and they have the potential to be widely used in the production of high-quality food. Chan seeds are presently used in a very limited manner as a food source; however, considering their high quality composition, they have the potential for a more extended use in the food industry. © 2011 Institute of Food Technologists®

  17. Photosynthetic rates of sun versus shade leaves of Hyptis emoryi Torr

    Energy Technology Data Exchange (ETDEWEB)

    Nobel, P.S.

    1976-08-01

    Leaves on a bush of Hyptis emoryi Torr, varied in length from less than 1 cm when development occurred in full sunlight (e.g. 40 Mjoules m/sup -2/) to over 7 cm when the total daily solar irradiance was less than 3 Mjoules m/sup -2/. The 1-cm sun leaves were 3-fold higher than the 7-cm shade leaves in chlorophyll per unit area, mesophyll thickness, and the internal to external leaf area ratio (A/sup mes//A). The higher A/sup mes//A caused a 1.2-cm leaf to have a 3-fold lower CO/sub 2/ liquid phase resistance than did a 7.1-cm leaf. Large thin shade leaves captured photosynthetically active radiation effectively (less than 7 percent passed through), but were not adapted to full sunlight. Specifically, when a 6.9-cm leaf was placed at 910 w m/sup -2/ for 30 min, its temperature exceeded that of the air by nearly 8/sup 0/C. For the common daytime air temperatures above 30/sup 0/C for this desert shrub, large shade leaves would have temperatures far in excess of that optimum for photosynthesis for H. emoryi, 29 to 32/sup 0/C.

  18. Alpha-pyrones and a 2(5H)-furanone from Hyptis pectinata.

    Science.gov (United States)

    Boalino, Dionne M; Connolly, Joseph D; McLean, Stewart; Reynolds, William F; Tinto, Winston F

    2003-12-01

    Three pyrones and a 2(5H)-furanone, designated pectinolides D-G, have been isolated from the dichloromethane extract of Hyptis pectinata. The metabolites were characterized on the basis of 1D and 2D NMR spectroscopic techniques. The pyrones were identified as 6S-[3S,6S-(diacetoxy)-5R-hydroxy-1Z-heptenyl]-5S-hydroxy-5,6-dihydro-2H-pyran-2-one (1)- pectinolide D, 6S-[3S,5R,6S-(triacetoxy)-1Z-heptenyl]-5S-acetoxy-5,6-dihydro-2H-pyran-2-one (2)- pectinolide E and 6S-[3S,5R,6S-(triacetoxy)-1Z-heptenyl]-5S-acetoxy-4R-methoxy-3,4,5,6-tetrahydro-4H pyran-2-one (3)- pectinolide F. The furanone was identified as [2'Z,5(1')Z] 5-(4'S,6'R,7'S-triacetoxy-2-octenylidene)-2(5H)-furanone (4)-pectinolide G.

  19. Potential of the bush mint, Hyptis suaveolens essential oil for personal protection against mosquito biting.

    Science.gov (United States)

    Abagli, Ayaba Z; Alavo, Thiery B C; Avlessi, Félicien; Moudachirou, Mansourou

    2012-03-01

    We studied the potential of the essential oil extract from the bush mint, Hyptis suaveolens, for use against mosquito biting under both laboratory and field conditions. In the laboratory, the repellency of various concentrations (1-6%) of the essential oil was assessed against Anopheles gambiae, based on a 15-min landing and biting on treated forearms of volunteers. In the laboratory, the percentage of mosquitoes landing on the forearm was 42, 33, 23, 23, 9, and 2 for 1%, 2%, 3%, 4%, 5%, and 6% essential oil concentration, respectively; and 92 and 91 for the solvent (isopropanol) and untreated control, respectively. The percentage of mosquitoes taking a blood meal was 22, 12, 13, 12, 5, and 3 for 1%, 2%, 3%, 4%, 5%, and 6% essential oil, respectively; and 52 and 51 for the solvent and control, respectively. In the field, the 6% essential oil repelled all mosquitoes immediately postapplication; this activity declined to 75% after 5 h. The repellent action of the 8% essential oil concentration was higher, 97% after 5 h. Based on these data, the essential oil of H. suaveolens appears to be a good candidate for use in the integrated management of mosquito vectors of disease.

  20. Hyptis pectinata essential oil: chemical composition and anti-Streptococcus mutans activity.

    Science.gov (United States)

    Nascimento, P F C; Alviano, W S; Nascimento, A L C; Santos, P O; Arrigoni-Blank, M F; de Jesus, R A; Azevedo, V G; Alviano, D S; Bolognese, A M; Trindade, R C

    2008-09-01

    The aim of the present study was to evaluate the anti-Streptococcus mutans activity of Hyptis pectinata essential oil, and present its promising potential against oral diseases. The essential oil of H. pectinata was obtained by hydrodistillation from dried leaves and analyzed by GC / MS. The effectiveness of this essential oil regarding the antimicrobial activity against several S. mutans strains was investigated by the agar diffusion and microdilution methods, and chlorohexidine was used as a standard control. The H. pectinata essential oil exhibited considerable inhibitory effect against either all the clinical isolates obtained from patients' saliva or the ATCC strains tested, with minimum inhibitory and bactericidal concentrations of 200 microg ml(-1). The study also compared the efficiency of the emulsifying agents Tween 20, Tween 80, dimethyl sulfoxide and propylene glycol in H. pectinata essential oil when tested against S. mutans. The data obtained confirmed the better inhibitory effect of the oil when using all tested diluents, although Tween 80 seemed to be more suitable for emulsification. According to our results, H. pectinata essential oil can be considered a promising alternative to chlorhexidine for the control of oral bacteria-related diseases and hygiene.

  1. Photosynthetic Rates of Sun versus Shade Leaves of Hyptis emoryi Torr. 1

    Science.gov (United States)

    Nobel, Park S.

    1976-01-01

    Leaves on a bush of Hyptis emoryi Torr. varied in length from less than 1 cm when development occurred in full sunlight (e.g. 40 Mjoules m−2) to over 7 cm when the total daily solar irradiance was less than 3 Mjoules m−2. The 1-cm sun leaves were 3-fold higher than the 7-cm shade leaves in chlorophyll per unit area, mesophyll thickness, and the internal to external leaf area ratio (Ames/A). The higher Ames/A caused a 1.2-cm leaf to have a 3-fold lower CO2 liquid phase resistance than did a 7.1-cm leaf. Large thin shade leaves captured photosynthetically active radiation effectively (less than 7% passed through), but were not adapted to full sunlight. Specifically, when a 6.9-cm leaf was placed at 910 w m−2 for 30 min, its temperature exceeded that of the air by nearly 8 C. For the common daytime air temperatures above 30 C for this desert shrub, large shade leaves would have temperatures far in excess of that optimum for photosynthesis for H. emoryi, 29 to 32 C. PMID:16659651

  2. Isoquercitrin isolated from Hyptis fasciculata reduces glioblastoma cell proliferation and changes beta-catenin cellular localization.

    Science.gov (United States)

    Amado, Nathália G; Cerqueira, Débora M; Menezes, Fabio S; da Silva, Joaquim Fernando Mendes; Neto, Vivaldo Moura; Abreu, Jose G

    2009-08-01

    Isoquercitrin isolated from the aerial parts of Hyptis fasciculata was evaluated according to its capacity to interfere with glioblastoma (Gbm) cell growth. Gbm cells were incubated with isoquercitrin, quercetin, or rutin at concentrations of 25, 50, and 100 mumol/l for 24, 48, and 72 h. Quercetin and rutin affected Gbm cell proliferation after treatment times of longer than 24 h. However, increasing concentrations of isoquercitrin inhibited 50% of Gbm cell proliferation at 24 h and further reached nearly 90% inhibition at 72 h. This effect did not affect cell morphology, cell viability, or cleaved capase-3 levels, indicating that isoquercitrin did not induce Gbm cell death. A marked reduction in cyclin D1 levels and an increase in p27 levels were observed when 100 micromol/l of isoquercitrin was added to Gbm cells. Interestingly, nuclear beta-catenin staining observed in a subpopulation of untreated Gbm cells was found in the cytoplasm after 100-micromol/l isoquercitrin treatment. Collectively, these data show that isoquercitrin reduces Gbm cell growth without inducing apoptosis, possibly by modulating the control of the cell cycle. Our data also suggest that beta-catenin-mediated signaling may be involved on the antiproliferative activity of isoquercitrin.

  3. Chemical composition and antifungal activity of Hyptis Suaveolens (L.) Poit leaves essential oil against Aspergillus species.

    Science.gov (United States)

    Moreira, Ana Carolina Pessoa; de Oliveira Lima, Edeltrudes; Wanderley, Paulo Alves; Carmo, Egberto Santos; de Souza, Evandro Leite

    2010-01-01

    This study aimed to identify the constituents of the essential oil from Hyptis suaveolens (L.) leaves using a Gas Chromatograph - Mass Spectrometer and assess its inhibitory effect on some potentially pathogenic Aspergilli (A. flavus, A. parasiticus, A. ochraceus, A. fumigatus and A. niger). Eucaliptol (47.64 %) was the most abundant component in the oil, followed for gama-ellemene (8.15 %), beta-pynene (6.55 %), (+)-3-carene (5.16 %), trans-beta-cariophyllene (4.69 %) and germacrene (4.86 %). The essential oil revealed an interesting anti-Aspergillus property characterized by a Minimum Inhibitory Concentration and Minimum Fungicidal Concentration of 40 and 80 µL/mL, respectively. The oil at 80 and 40 µL/mL strongly inhibited the mycelial growth of A. fumigatus and A. parasiticus along 14 days. In addition, at 10 and 20 µL/mL the oil was able to cause morphological changes in A. flavus as decreased conidiation, leakage of cytoplasm, loss of pigmentation and disrupted cell structure suggesting fungal wall degeneration. These findings showed the interesting anti-Aspergillus property of H. suaveolens leaves essential oil supporting its possible rational use as alternative source of new antifungal compounds to be applied in the aspergillosis treatment.

  4. Analysis of genetic diversity of Hyptis pectinata (L.) Poit. plants using ISSR markers.

    Science.gov (United States)

    Feitosa-Alcantara, R B; Silva, A V C; Blank, A F; Almeida, C S; Alvares-Carvalho, S V; Arrigoni-Blank, M F

    2017-08-17

    Hyptis pectinata, popularly known as 'sambacaitá' or 'canudinho', is a medicinal and aromatic species widely used in the Brazilian Northeast. In Sergipe, the excessive extraction of natural resources may reduce the genetic variability of native plants. Thus, molecular markers have frequently been applied to the characterization of genetic diversity as the basis for germplasm conservation and breeding programs. The objective of the present study was to evaluate the genetic diversity of H. pectinata plants collected in different municipalities of the State of Sergipe using ISSR molecular markers. Thirty-four primers were tested, nine of which were selected for providing reproducible and analyzable amplification products, resulting in 67 polymorphic bands. The expected heterozygosity ranged from 0.32 to 0.45, with a mean of 0.39. Polymorphism information content was of 0.49, which classifies the markers as moderately informative. A dendrogram was constructed using unweighted pair group method with arithmetic mean, forming three clusters: Cluster I (79 plants); Cluster II (4 plants); and Cluster III (2 plants). Jaccard's similarity coefficients ranged from 0.06 to 0.98. The plants SAM-117 and SAM-119 presented greater similarity. Conversely, SAM-107 and SAM-171 were the most genetically distant. In general, H. pectinata plants collected in the State of Sergipe presented low to moderate genetic diversity.

  5. Inhibition of quorum sensing mediated biofilm development and virulence in uropathogens by Hyptis suaveolens.

    Science.gov (United States)

    Salini, Ramesh; Sindhulakshmi, Muthukrishnan; Poongothai, Thirumaran; Pandian, Shunmugiah Karutha

    2015-04-01

    Bacterial urinary tract infections (UTIs) are the most common nosocomial infections, accounting for about 40 % of all hospital-acquired infections. The bacterial spectrum of nosocomial UTIs is broad and the treatment of UTIs is becoming difficult owing to the emergence of drug resistance. Therefore, it is reasonable to investigate novel and alternative therapeutic strategies to treat UTIs. Since UTIs are caused by uropathogens with quorum sensing (QS)-dependent biofilm forming abilities, interruption of QS systems may be a novel approach to combat drug resistance. In the present study, a methanol extract (and hexane extract derived from it) of the medicinal plant Hyptis suaveolens (L.) were shown to have anti-QS activity against the biosensor strain Chromobacterium violaceum (ATCC 12472). Furthermore, the hexane extract of H. suaveolens (HEHS) inhibited biofilm formation by uropathogens such as Escherichia coli, Proteus vulgaris, Proteus mirabilis, Klebsiella pneumoniae and Serratia marcescens. HEHS promotes the loosening of biofilm architecture and strongly inhibits in vitro biofilm formation by uropathogens, which was more apparent from microscopic images. In addition to this, HEHS reduces the production of QS-dependent virulence factors like protease and hemolysin, along with motility. The partial purification and GC-MS analysis of the active fraction revealed the presence of several therapeutically important compounds which may synergistically act on the uropathogens and possibly reduce the QS-dependent phenotypes. These findings suggest HEHS as potential phytotherapeutic agent which can be employed to formulate protective strategies against biofilm linked infections caused by uropathogens.

  6. Phenolic diterpenes derived from Hyptis incana induce apoptosis and G(2)/M arrest of neuroblastoma cells.

    Science.gov (United States)

    Tabata, Keiichi; Kim, Myongjun; Makino, Mitsuko; Satoh, Mitsuru; Satoh, Yoshio; Suzuki, Takashi

    2012-11-01

    Neuroblastoma is one of the most commonly encountered solid tumors in the pediatric age group, and the prognosis of patients with advanced neuroblastoma is very poor. In this study, the antitumor effects of five phenolic diterpenes derived from Hyptis incana (Lamiaceae), a Brazilian medicinal plant, were examined on neuroblastoma cells. Cytotoxicity was assessed by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Apoptotic nuclear shrinkage was monitored by Hoechst 33342 staining. The cell-cycle status was evaluated by flow cytometry and protein alterations were monitored by western blotting. Differentiated cells were photographed and counted in a randomized fashion. All of the examined compounds exhibited significant cytotoxicity towards the neuroblastoma cells. In particular, 7-ethoxyrosmanol had a high degree of efficacy. Nuclear condensation and degradation of procaspase-3 and -9 were observed after treatment of the cells with these compounds. Moreover, phenolic diterpenes induced cell-cycle arrest in the G(2)/M phase. Rosmanol and epirosmanol tended to induce differentiation. Phenolic diterpenes isolated from H. incana have multiple antitumor effects on neuroblastoma cells.

  7. Morphological, agronomical and pharmacological characterization of Hyptis pectinata (L. Poit germplasm

    Directory of Open Access Journals (Sweden)

    M.F. Arrigoni-Blank

    Full Text Available Several species of the genus Hyptis have presented medicinal characteristics and are frequently indicated to treat gastro intestinal infections, gramps and pain, as well as for skin infections. Three harvests were carried out using six H. pectinata accessions, and the following characteristics were evaluated: plant height, canopy diameter, leave length (L and width (W, L/W relation and dry weight of leaves and stem. The accession SAM006 have presented highest leave dry matter yield when the three harvests were summed. The aqueous leaf extracts of the same six H. pectinata accessions were tested for anti-edematogenic effect using the carrageenin-induced rat paw edema at doses of 200, 400 and 600 mg/kg. The extracts administered orally had a significant anti-edematogenic effect. The dose of 200 mg/kg of accessions SAM004 and SAM005 had a stronger effect reducing the edema by 19.6% and 15.4%, respectively. Similar results were obtained using higher dosages of the aqueous extract of the accessions SAM002, SAM003 and SAM006. It can be concluded that the accessions SAM004, SAM005 and SAM006 are promising genotypes for a plant breeding program that aims the development of a cultivar with highest leave yielding and anti-edematogenic effect.

  8. Influence of storage time and temperature on the chemical composition of the essential oil of Hyptis pectinata L. Poit

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    A.S. JESUS

    2016-01-01

    Full Text Available ABSTRACT Hyptis pectinata, popularly known as “sambacaitá”, is a native medicinal herb used by local people for treating disease. Its pharmacological, antimicrobial, and medicinal properties have been reported in several studies. The essential oil of the leaves is rich in terpene compounds responsible for its properties. Because there are factors that can influence the chemical composition of essential oils, the objective of this study was to evaluate the influence of storage on Hyptis pectinata essential oil for 360 days at two different temperatures. The essential oil was distilled from dried leaves of seven-month-old plants using the hydrodistillation method, and the chemical composition was determined using GC-MS and GC/FID. The effects of storage on the essential oil Hyptis pectinata were tested over the course of a year (0, 15, 30, 60, 90, 120, 150, 180, 240, 300, and 360 days, using two temperatures: room (±32ºC and freezer (-20ºC. The predominant compounds in the essential oil are β–caryophyllene, caryophyllene oxide, and germancrene D. The concentration of the chemical constituents of the essential oil varied depending on the storage temperature, and over the 360 days. The storage of the essential oil at room temperature over one year resulted in higher concentrations of β-elemene, α-copaene, germacrene D, caryophyllene oxide, and (E,E-α-farnesene and lower content of α-humulene and β-caryophyllene, compared to the results obtained from storing the essential oil in a freezer. These results indicate that the essential oil should be stored in a freezer, which provides greater stability to the concentration of the chemical constituents.

  9. The Acute Toxicity Test of Methanolic Extract of Hyptis pectinata Poit on Liver Balb/c Mice

    Science.gov (United States)

    Suzery, M.; Cahyono, B.; Astuti, P.

    2017-02-01

    Plants from Lamiaceae family has almost entirely reported having physiological activities. One of them is Hyptis pectinata Poit plant. Research on the toxicity of Hyptis pectinata needs to be done to protect people from the possibility of its harmful effects. This study aim to know the acute toxicity effects of Hyptis pectinata extract (HPE) on liver of Balb/c mice. This research was a laboratory experimental study using the post test only controlled group design. Balb/c mice were randomly divided into 4 groups. K (control group), P1, P2 and P3 (treatment groups; given HPE 200mg/kgBW, 1000 mg/kgBW, and 5000 mg/kgBW, respectively). The extract was orally given with gastric tube on the first day and the mice were terminated at the 8th day then the livers were observed. The Kruskal-Wallis test for macroscopic morphological and volume of the liver showed there were no significant difference with p=0.406 and p=0.054. The highest liver histopathological score was in P3 group. The Kruskal-Wallis test showed significantly difference (p=0.000). Continued with Mann-Withney test that showed a significant difference in K-P1 (p=0.009), K-P2 (p=0.009), K-P3 (p=0.009), P1-P2 (p=0.028), and P1-P3 (p=0.009). In conclusion, the HPE is safe to use which has no complication with liver of mice.

  10. Chemical Composition and Antibacterial Activity of Essential Oil Hyptis suaveolens (L.) Poit. (Lamiaceae) from the Venezuelan Plains

    OpenAIRE

    Nurby Ríos Tesch; Ramón Márquez Yánez; Xiogelis Mendoza Rojas; Luis Rojas-Fermín; Judith Velasco Carrillo; Tulia Díaz; Flor Mora Vivas; Carlos Yánez Colmenares; Pablo Meléndez González

    2015-01-01

    El aceite esencial (AE) obtenido de las hojas y flores de Hyptis suaveolens (L.) Poit. recolectado en Guasdualito, Estado Apure, Venezuela, fue obtenido por hidrodestilación empleando una trampa de Clevenger. El aceite se analizó mediante cromatografía de gases acoplada a espectrometría de masas (GC/MS), se identificaron treinta y cinco compuestos en las hojas (91.9% de la muestra) y treinta compuestos en las flores (97.0% de la muestra), con los siguientes compuestos principales: 1,8-cineol ...

  11. Rosmarinic Acid and Its Methyl Ester as Antimicrobial Components of the Hydromethanolic Extract of Hyptis atrorubens Poit. (Lamiaceae

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    Amin Abedini

    2013-01-01

    Full Text Available Primary biological examination of four extracts of the leaves and stems of Hyptis atrorubens Poit. (Lamiaceae, a plant species used as an antimicrobial agent in Guadeloupe, allowed us to select the hydromethanolic extract of the stems for further studies. It was tested against 46 microorganisms in vitro. It was active against 29 microorganisms. The best antibacterial activity was found against bacteria, mostly Gram-positive ones. Bioautography enabled the isolation and identification of four antibacterial compounds from this plant: rosmarinic acid, methyl rosmarinate, isoquercetin, and hyperoside. The MIC and MBC values of these compounds and their combinations were determined against eight pathogenic bacteria. The best inhibitory and bactericidal activity was found for methyl rosmarinate (0.3 mg/mL. Nevertheless, the bactericidal power of rosmarinic acid was much faster in the time kill study. Synergistic effects were found when combining the active compounds. Finally, the inhibitory effects of the compounds were evaluated on the bacterial growth phases at two different temperatures. Our study demonstrated for the first time antimicrobial activity of Hyptis atrorubens with identification of the active compounds. It supports its traditional use in French West Indies. Although its active compounds need to be further evaluated in vivo, this work emphasizes plants as potent sources of new antimicrobial agents when resistance to antibiotics increases dramatically.

  12. HPLC-DAD analysis, antinociceptive and anti-inflammatory properties of the ethanolic extract of Hyptis umbrosa in mice.

    Science.gov (United States)

    Dos Anjos, Klécia S; Araújo-Filho, Heitor G; Duarte, Marcelo C; Costa, Vicente C O; Tavares, Josean F; Silva, Marcelo S; Almeida, Jackson R G S; Souza, Nathália A C; Rolim, Larissa A; Menezes, Irwin R A; Coutinho, Henrique D M; Quintans, Jullyana S S; Quintans-Júnior, Lucindo J

    2017-01-01

    Hyptis umbrosa (syn. Mesosphaerum sidifolium) (Lamiaceae Family) has been used to treat several conditions such as gastrointestinal disorders, skin infections, nasal congestion, fever and cramps. The objective of this study was to evaluate the chemical composition, analgesic and anti-inflammatory profiles of ethanol extract from leaves of Hyptis umbrosa (EEB). HPLC-DAD was used to determine the fingerprint chromatogram of the extract. Male Swiss mice were orally pretreated with EEB (100, 200 or 400 mg/kg; 60 min before initiating algesic stimulation) and antinociceptive activity was assessed using the acetic acid-induced writhing model, formalin test and hyperalgesia induced by glutamate or capsaicin. Also, peritonitis was induced by the intrathoracic injection of carrageenan to quantify the total number of leukocytes. The presence of phenolic compounds in the extract was confirmed using HPLC-DAD. The treatment with EEB, at all doses, produced a significant analgesic effect against acetic acid-induced antinociceptive activity. In the formalin test, only the 400-mg/kg-dose of EEB had a significant effect in the first phase. However, all doses tested were able to reverse nociception in the second phase. The effect of all doses of EEB also showed a significant antinociceptive effect in the glutamate and capsaicin tests and inhibited the carrageenan-induced leukocyte migration to the peritoneal cavity. The present study suggests that the EEB possesses peripheral analgesic action and showed potential in reducing the spreading of the inflammatory processes. Also, it seems to be related with vanilloid and glutamate receptors.

  13. Rosmarinic Acid and Its Methyl Ester as Antimicrobial Components of the Hydromethanolic Extract of Hyptis atrorubens Poit. (Lamiaceae).

    Science.gov (United States)

    Abedini, Amin; Roumy, Vincent; Mahieux, Séverine; Biabiany, Murielle; Standaert-Vitse, Annie; Rivière, Céline; Sahpaz, Sevser; Bailleul, François; Neut, Christel; Hennebelle, Thierry

    2013-01-01

    Primary biological examination of four extracts of the leaves and stems of Hyptis atrorubens Poit. (Lamiaceae), a plant species used as an antimicrobial agent in Guadeloupe, allowed us to select the hydromethanolic extract of the stems for further studies. It was tested against 46 microorganisms in vitro. It was active against 29 microorganisms. The best antibacterial activity was found against bacteria, mostly Gram-positive ones. Bioautography enabled the isolation and identification of four antibacterial compounds from this plant: rosmarinic acid, methyl rosmarinate, isoquercetin, and hyperoside. The MIC and MBC values of these compounds and their combinations were determined against eight pathogenic bacteria. The best inhibitory and bactericidal activity was found for methyl rosmarinate (0.3 mg/mL). Nevertheless, the bactericidal power of rosmarinic acid was much faster in the time kill study. Synergistic effects were found when combining the active compounds. Finally, the inhibitory effects of the compounds were evaluated on the bacterial growth phases at two different temperatures. Our study demonstrated for the first time antimicrobial activity of Hyptis atrorubens with identification of the active compounds. It supports its traditional use in French West Indies. Although its active compounds need to be further evaluated in vivo, this work emphasizes plants as potent sources of new antimicrobial agents when resistance to antibiotics increases dramatically.

  14. Bioactive 5,6-Dihydro-α-pyrone Derivatives from Hyptis brevipes

    Science.gov (United States)

    Deng, Ye; Balunas, Marcy J.; Kim, Jeong-Ah; Lantvit, Daniel D.; Chin, Young-Won; Chai, Heebyung; Sugiarso, Sugeng; Kardono, Leonardus B. S.; Fong, Harry H. S.; Pezzuto, John M.; Swanson, Steven M.; Carcache de Blanco, Esperanza J.; Kinghorn, A. Douglas

    2010-01-01

    Six new 5,6-dihydro-α-pyrone derivatives (1–6), namely, brevipolides A–F, together with seven known compounds, including a 5,6-dihydro-α-pyrone derivative (7), three flavonoids, a steroid glycoside, and two triterpenoids, were isolated from the entire plant of Hyptis brevipes. Compounds 1–7 were assigned with the absolute configuration, 5R, 6S, 7S, and 9S, as elucidated by analysis of data obtained from their CD spectra and by Mosher ester reactions. Compounds 2, 6, and 7 exhibited ED50 values of 6.1, 6.7 and 3.6 μM against MCF-7 cells, and compounds 1, 2, 6, and 8 (the known 5,6,3′-trihydroxy-3,7,4′-trimethoxyflavone) gave ED50 values of 5.8, 6.1 7.5, and 3.6 μM against HT-29 cells, respectively. However, no significant cytotoxicity was found against Lu1 cells for any of the compounds isolated. When these compounds were subjected to evaluation in a panel of mechanism-based in vitro assays, compound 7 were found to be active in an enzyme-based ELISA NF-κB assay, with an ED50 value of 15.3 μM. In a mitochondrial transmembrane potential assay, compounds 3, 7, and 8 showed ED50 values of 8.5, 75, and 310 nM, respectively. However, no potent activity was found in a proteasome inhibition assay for any of the isolated compounds. PMID:19422206

  15. Production of podophyllotoxin from roots and plantlets of Hyptis suaveolens cultivated in vitro.

    Science.gov (United States)

    Velóz, Rafael A; Cardoso-Taketa, Alexandre; Villarreal, María Luisa

    2013-04-01

    Hyptis suaveolens was an important source of food and medicines in pre-hispanic Mιxico and is actually used popularly to treat respiratory and skin diseases, fever, pain, and cramps, between other ailments. In 2008 the presence of podophyllotoxin (PTOX) was reported in this plant. To establish in vitro cultures of H. suaveolens able to produce PTOX. Explants of H. suaveolens were cultivated in Murashige and Skoog (MS) medium supplemented with different concentrations of the phytohormones 6-benzylaminopurine (6-BAP), 2,4-dichlorophenoxyacetic acid (2,4-D), 1-naphthaleneacetic acid (NAA) and kinetin (Kin), in order to induce the production of podophyllotoxin. Root cultures without hormones were also established and the quantification of PTOX was performed by HPLC analysis. The presence of growth regulators during in vitro cultivation of H. suaveolens, provoked morphological variations in explants, and induced the accumulation of different levels of PTOX. Roots grown without phytohormones accumulated PTOX at 0.013% dry weight (DW), while in three of the callus cultures cell lines growing together with roots, PTOX accumulated at concentrations of 0.003, 0.005 and 0.006% DW when NAA was combined with either Kin or BAP. In wild plant material PTOX was present in trace amounts in the aerial parts, while in the roots it was found at 0.005% DW. This study demonstrated that although it is possible to obtain PTOX in a variety of in vitro cultures of H. suaveolens, in vitro roots grown without the addition of growth regulators were better producers of PTOX.

  16. Bioactive 5,6-dihydro-alpha-pyrone derivatives from Hyptis brevipes.

    Science.gov (United States)

    Deng, Ye; Balunas, Marcy J; Kim, Jeong-Ah; Lantvit, Daniel D; Chin, Young-Won; Chai, Heebyung; Sugiarso, Sugeng; Kardono, Leonardus B S; Fong, Harry H S; Pezzuto, John M; Swanson, Steven M; de Blanco, Esperanza J Carcache; Kinghorn, A Douglas

    2009-06-01

    Six new 5,6-dihydro-alpha-pyrone derivatives (1-6), namely, brevipolides A-F, together with seven known compounds, including a 5,6-dihydro-alpha-pyrone derivative (7), three flavonoids, a steroid glycoside, and two triterpenoids, were isolated from the entire plant of Hyptis brevipes. Compounds 1-7 were assigned with the absolute configuration 5R, 6S, 7S, and 9S, as elucidated by analysis of data obtained from their CD spectra and by Mosher ester reactions. Compounds 2, 6, and 7 exhibited ED(50) values of 6.1, 6.7, and 3.6 microM against MCF-7 cells, and compounds 1, 2, 6, and 8 (the known 5,6,3'-trihydroxy-3,7,4'-trimethoxyflavone) gave ED(50) values of 5.8, 6.1, 7.5, and 3.6 microM against HT-29 cells, respectively. However, no significant cytotoxicity was found against Lu1 cells for any of the compounds isolated. When these compounds were subjected to evaluation in a panel of mechanism-based in vitro assays, compound 7 was found to be active in an enzyme-based ELISA NF-kappaB assay, with an ED(50) value of 15.3 microM. In a mitochondrial transmembrane potential assay, compounds 3, 7, and 8 showed ED(50) values of 8.5, 75, and 310 nM, respectively. No potent activity was found in a proteasome inhibition assay for any of the isolated compounds.

  17. Molecular dimensions and structural features of neutral polysaccharides from the seed mucilage of Hyptis suaveolens L.

    Science.gov (United States)

    Praznik, Werner; Čavarkapa, Andrea; Unger, Frank M; Loeppert, Renate; Holzer, Wolfgang; Viernstein, Helmut; Mueller, Monika

    2017-04-15

    The seed mucilage of Hyptis suaveolens L. includes acid - and neutral heteropolysaccharides in a ratio of about 1:1. The anionic charged fraction responsible for swelling and viscous behaviour possesses an average molar mass of Mw=350kg/mol, Mn=255kg/mol. The neutral polysaccharide fraction shows an average molar mass of Mw=47kg/mol and Mn=28kg/mol and is composed of d-Galp-, d-Glcp- and d-Manp residues in a molar ratio of about 3:2:1. The structural features present galactoglucan (30%) and galactoglucomannan (70%) with a high level of terminal β-linked d-Galp residues (18%). Structural details of galactoglucomannan are derived by combined enzymatic and chemical methods as well as NMR spectroscopy. Sequences of octa/nonasaccharide β-d-Glcp-(1→4)[β-d-Galp-(1→2)-α-d-Galp-(1→6)]-β-d-Manp-(1→4)-β-d-Glcp-(1→4)-β-d-Glcp-(1→4)[β-d-Galp-(1→2)-α-d-Galp-(1→6)]-β-d-Manp and lower mass tetrasaccharide repeating units β-d-Glcp-(1→4)[β-d-Galp-(1→2)-α-d-Galp-(1→6)]-β-d-Manp were found. The level of the prebiotic activity is related to the availability of β-linked d-Galp residues in the side chains of the molecules. Copyright © 2016 Elsevier Ltd. All rights reserved.

  18. Antioxidant and neuroprotective activities of Hyptis suaveolens (L.) Poit. against oxidative stress-induced neurotoxicity.

    Science.gov (United States)

    Ghaffari, Hadi; Ghassam, Behrouz Jalali; Chandra Nayaka, S; Ramachandra Kini, K; Prakash, H S

    2014-04-01

    The present study was carried out to investigate the antioxidant and neuroprotective effects of Hyptis suaveolens methanol extract (HSME) using various in vitro systems. The total phenol and flavonoids contents of the HSME were quantified by colorimetric methods. The HSME extract exhibited potent antioxidant activity as determined by 2,20-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt, 2,2-diphenyl-1-picrylhydrazyl, and ferric reducing antioxidant power assays. The neuroprotective activity of HSME was determined on mouse N2A neuroblastoma cells using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, lactate dehydrogenase, intracellular ROS assays, and upregulation of brain neuronal markers at genetic level. The N2A cells were pretreated with different concentrations (0.5, 1, 1.5, and 2 mg/ml) of the extract and then exposed to H2O2 to induce oxidative stress and neurotoxicity. The survival of the cells treated with different concentrations of HSME and H2O2 increased as compared to cells exposed only to H2O2 (47.3 %) (p < 0.05). The HSME also dose-dependently reduced LDH leakage and intracellular ROS production (p < 0.05). Pretreatment with HSME promotes the upregulation of tyrosine hydroxylase (2.41-fold, p < 0.05), and brain-derived neurotrophic factor genes (2.15-fold, p < 0.05) against H2O2-induced cytotoxicity in N2A cells. Moreover, the HSME showed antioxidant activity and decreased neurotoxicity. These observations suggest that HSME have marked antioxidant and neuroprotective activities.

  19. Hyptis pectinata gel prevents alveolar bone resorption in experimental periodontitis in rats

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    Mônica S. Paixão

    Full Text Available Hyptis pectinata (L. Poit., Lamiaceae, is an aromatic, abundant and broadly used plant species in Sergipe to treat oral and gastrointestinal pain and inflammation. The aim of the present study was to analyze the relation between periodontitis and changes in the corporal mass and alveolar bone structure after induction of experimental periodontal disease in rat treated or not treated with H. pectinata gel at 5% (GS5% and 10% (GS10%, comparing their effects with doxycycline gel at 10% (D10%, positive control, vehicle gel (negative control and a group with experimental periodontal disease, but non-treated. The gels were locally applied in the gingival region immediately after the experimental periodontal disease induction by ligature (3×/day, 11 days. Bone destruction was determined through clinical exam, histopathological analysis and cone beam computed tomography of the experimental animals (n = 36. After 11 days of periodontitis induction, all groups that received ligature presented a decrease in the corporal mass, except to the naïve group (without experimental periodontal disease (p < 0.05. Computed tomography results have shown healthy bone structure in the group I and bone resorption for the test groups. Histopathological analysis confirmed the healthy bone structure for naïve group animals, while the test groups exhibited bone loss in several degrees. In particular, the non-treated group animals had an intense inflammatory process. When the periodontium of the animals treated with GS10% was histopathologically analyzed, insertion periodontium was preserved. The results for these groups were significantly different of the vehicle group (p < 0.05. According to the results, the gel based in the aqueous extract of H. pectinata at 10% can prevent bone loss in experimental periodontal disease similarly to doxycycline 10%.

  20. Impact of light quality on flavonoid production and growth of Hyptis marrubioides seedlings cultivated in vitro

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    Rita Cassia N. Pedroso

    Full Text Available ABSTRACT Hyptis marrubioides Epling, Lamiaceae, a species from Brazilian Cerrado, has been used against gastrointestinal infections, skin infections, pain, and cramps. Herein, H. marrubioides seedlings were cultured in vitro under different wavelengths (white, blue, green, red, and yellow with 50 µmol m-2 s-1 irradiance and a 16-h photoperiod. After 20 and 30 days of cultivation, shoot length, leaf number, fresh mass, and dry mass were evaluated. The flavonoid rutin content was determined by the HPLC-DAD method. The shoots were longer in plants cultivated under yellow (16.603 ± 0.790 cm, 1.8-fold, red (15.465 ± 0.461 cm, 1.7-fold, and green (14.677 ± 0.737 cm, 1.6-fold lights than in control plants exposed to white light (9.203 ± 0.388 cm. The number of leaves increased in plants exposed to red (23.425 ± 1.138, 1.1-fold and green (22.725 ± 1.814, 1.1-fold lights, compared to control plants (20.133 ± 0.827. Fresh (0.665 ± 0.048 g, 1.2-fold and dry (0.066 ± 0.005 g, 1.3-fold mass of seedlings were the highest in seedlings grown under red light, compared to seedlings grown under white light (0.553 ± 0.048 and 0.028 ± 0.004, respectively. However, rutin production was higher under white (0.308 mg g-1 of dry weight and blue lights (0.298 mg g-1 of dry weight. Thus, red light induces plant growth and increases leaf number and dry weight in in vitro-cultivated H. marrubioides, whereas blue and white lights promote the greatest rutin accumulation.

  1. Evaluation of antipyretic activity of ethanolic extract of plant Geniosporum prostratum (L. Benth. Bark

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    Anil Kumar Singhal

    2011-01-01

    Full Text Available Background: The plant Geniosporum prostratum (L. Benth. belongs to the family of "Lamiaceae," which is widely available in Tamil Nadu. Traditionally, plant extract is used to treat fever and common cold for children. The plant has not been yet studied pharmacologically for antipyretic activity. Aim: The aim of the present study was to evaluate the antipyretic activity of alcoholic extract of the bark of plant G. prostratum (L. Benth. Materials and Methods: A total of 24 healthy white albino rats weighing 200 to 250 g were taken and divided into four groups of six animals each. The initial rectal temperature of each animal was recorded by digital thermometer and its hourly variation was noted for 4 hours. The pyrexia was induced by injecting a suspension of 12% of brewer′s yeast (at the dose 1 ml/100 g of animal weight in normal saline subcutaneously below the nape of neck. Ethanolic extract was given orally to groups II and III at the dose 100 and 200 mg/kg body weight, respectively. Statistical Analysis: The results are presented as mean΁SEM. Statistical analysis of data was performed using Dunnett′s test to study the difference among the mean. Results: The difference in temperature between 0 hour and respective time interval was found out by statistical method. The potency of extract to bring down the temperature was compared with that of the control group. The present results showed that ethanolic extract of bark of G. prostratum plant possess a significant antipyretic effect in yeast-induced elevation of body temperature in experimental rats. It was revealed that the extract showed dose-dependent antipyretic activity. At a dose of 200 mg/kg, it showed significant antipyretic activity. Conclusion: The ethanolic extract of G. prostratum (L. Benth. plant has significant antipyretic activity when compared with the standard drug. So, it can be recommended for further studies.

  2. Evaluation of antifungal activity of standardized extract of Salvia rhytidea Benth. (Lamiaceae) against various Candida isolates.

    Science.gov (United States)

    Salari, S; Bakhshi, T; Sharififar, F; Naseri, A; Ghasemi Nejad Almani, P

    2016-12-01

    Salvia species have long been described in traditional medicine for various indications. Owing to the widespread use of this genus by ethnic populations, especially for various infections ranging from skin disease to gastrointestinal disorders, we were encouraged to determine whether Salvia rhytidea could be effective against fungal infections. Given the increased incidence of candidiasis in the past decade, limits on the use of antifungal drugs, emergence of azole-resistant Candida species and increased incidence of treatment failures, it is necessary to identify a novel agent with antifungal properties. Aim of the study was to evaluate the antifungal properties of S. rhytidea against various Candida isolates. In this study, at first rosmarinic acid content of plant extract was determined. A total of 96 Candida isolates were tested, including the following species: Candida albicans (n=42), Candida glabrata (n=16), Candida tropicalis (n=11), Candida krusei (n=9), Candida parapsilosis (n=9), Candida lusitaniae (n=7) and Candida guilliermondii (n=2). The in vitro antifungal activity of methanolic extracts of S. rhytidea Benth. was evaluated against Candida isolates and compared with that of the standard antifungal drug nystatin by using a broth microdilution method, according to CLSI. Phytochemical screening results showed that the methanolic extract of S. rhytidea Benth. was rich in flavonoids and tannins. The minimal inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) values of S. rhytidea Benth. ranged from 3.125 to>100μg/ml and 6.25 to>100μg/ml respectively. The growth inhibition value displayed that C. tropicalis, C. krusei and C. albicans isolates were most susceptible to S. rhytidea. Findings show that S. rhytidea possesses an antifungal effect against Candida isolates. Copyright © 2016 Elsevier Masson SAS. All rights reserved.

  3. Chemical Composition and Antimicrobial Activity of Essential Oil of Lepechiniaradula Benth Epling

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    Vladimir Morocho

    2017-01-01

    Full Text Available The essential oil (EO was obtained by hydrodistillation from the aerial parts of Lepechiniaradula Benth Epling (Lamiaceae from Ecuador. Thirty-four compounds accounting to 93.4% of the total oil were identified. The main constituents of the essential oil were δ-3-carene (19.9%, β-pinene (17.0%, (E-β-caryophyllene (9.7% and (E-E-α-farnesene (9.4%. The essential oil of L.radula possessed strong antifungal activity against Trichophytonrubrum (ATCC® 28188 and Trichophytonmentagrophytes (ATCC® 28185.

  4. Estudo anatômico do xilema secundário de Mimosa sparsa Benth.

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    Adonis Maccari

    2009-09-01

    Full Text Available Normal 0 21 false false false MicrosoftInternetExplorer4 Os caracteres microscópicos da madeira de Mimosa sparsa Benth. são descritos, com base em 30 dados quantitativos de sua estrutura anatômica. A descrição ‚ ilustrada com fotomicrografias tomadas nos três planos anatômicos. A estrutura da madeira é comparada com referências da literatura, em análise taxonômica e filogenética.

  5. Phytochemical analysis of bark from Helietta apiculata Benth and antimicrobial activities.

    Science.gov (United States)

    Fernandes, Tanize S; Copetti, Daniele; do Carmo, Gabriele; Neto, Alexandre T; Pedroso, Marcelo; Silva, Ubiratan F; Mostardeiro, Marco A; Burrow, Robert E; Dalcol, Ionara I; Morel, Ademir F

    2017-09-01

    Extraction and characterization of natural products from the bark of the trunk of Helietta apiculata Benth (Rutaceae) afforded nine alkaloids, eight furoquinoline and one quinolone, limonine, three cinnamic acid derivatives, three neolignans, tetracosanoic acid, six coumarins, of which apiculin A and apiculin B (neolignans), and tanizin (coumarin) are previously undescribed compounds. The structures of all compounds were determined by spectroscopic methods, and the crystal structures of two of the newly undescribed compounds, apiculin A and apiculin B, were determined by X-ray analysis. Extracts and pure compounds isolated from Helietta apiculata showed promising antimicrobial activities. Copyright © 2017 Elsevier Ltd. All rights reserved.

  6. Seed morphology of section Edentulae Benth. of the genus Pedicularis L. (Orobanchaceae Vent.) in the Eastern Europe

    OpenAIRE

    Peregrym O.; Futorna O.

    2013-01-01

    The seeds ultrastructure of the five species of the section EdentulaeBenth. of the Eastern Europe were studied using scanning electron microscopy. The seeds descriptions of surface of the species of this section were presented in this article. These species are heterogeneous by characteristics of seeds surface, which we consider as valuable addition features for identification within the section.

  7. Penstemon lanceolatus Benth. or P. ramosus Crosswhite in Arizona and New Mexico, a peripheral or endemic species?

    Science.gov (United States)

    J. L. Anderson; S. Richmond-Williams; O. Williams

    2007-01-01

    The red-flowered member of Penstemon sect. Chamaeleon from southeastern Arizona and southwestern New Mexico has been treated taxonomically both as part of the Mexican species, P. lanceolatus Benth., and as a separate species, P. ramosus Crosswhite. Under the former treatment the Arizona and New Mexico populations are peripheral populations of a primarily Mexican...

  8. Perennial peanut (Arachis glabrata Benth.) contains polyphenol oxidase (PPO) and PPO substrates that can reduce post-harvest proteolysis

    Science.gov (United States)

    Studies of perennial peanut (Arachis glaburata Benth.) suggest its hay and haylage have higher levels of rumen undegraded protein (RUP) than other legume forages such as alfalfa. Higher RUP can result in more efficient utilization of nitrogen by ruminant animals with positive economic and environmen...

  9. Vascular Reactivity Concerning Orthosiphon stamineus Benth-Mediated Antihypertensive in Aortic Rings of Spontaneously Hypertensive Rats

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    Nurul Maizan Manshor

    2013-01-01

    Full Text Available Orthosiphon stamineus Benth has been traditionally used to treat hypertension. The study aimed to investigate the vascular reactivity of water extract (WOS and water : methanolic (1 : 1 extract (WMOS of Orthosiphon stamineus Benth and AT1 receptors blocker in the mechanisms of antihypertensive mediated by α1-adrenergic receptor and EDNO and PGI2 releases in the SHR aortic rings. SHR (230–280 g were divided into four groups: control, WOS, WMOS, and losartan. After being fed orally for 14 days, the aorta was harvested and subjected to PE (10−9 to 10−5 M and ACh (10−9 to 10−5 M with and without L-NAME (100 µM and indomethacin (10 µM, respectively. WOS, WMOS, and losartan significantly reduced the contractile responses to PE intact suggesting the importance of endothelium in vasorelaxation. Losartan significantly enhanced the ACh-induced vasorelaxation. L-NAME significantly inhibited the ACh-induced relaxation in all groups. Indomethacin enhanced ACh-induced vasorelaxation in WMOS. Collectively, Orthosiphon stamineus leaves extract reduced vasoconstriction responses by the alteration of α1-adrenergic and AT1 receptors activities. The involvement of EDNO releases was clearly observed in this plant. In WOS, PGI2 releases might not participate in the ACh-induced vasorelaxation. However, in WMOS, enhancement of vasorelaxation possibly due to continuous release of PGI2.

  10. Efficacy of Pcroriza kurrooa benth in experimentally induced Hepato-toxicity in Cross-bred calves

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    V.R. Talmale

    2010-02-01

    Full Text Available Alcoholic extract of roots of picrorhiza kurrooa Benth was screened for its photochemical & hepato protective activity by metabolic (biochemical profile for hepato-protective activity by invasive blood sampling in calves. The mean extractability percentage obtained was 40.5. Phyto-chemical study revealed that the presence of reducing sugar, glycosides & saponins. The level of SGOT (AST, SGPT (ALT, alkaline phosphates, bilirubin increased significantly while the level of protein, albumin, globulin and glucose decreased significantly and moderate but not significant increase in cholesterol level in serum in calves treated with CC14 (thrice a week for two weeks, intra ruminally were observed. The bio-chemical alterations might be due to damage of liver cells and impairment in its functions. The animals treated with alcoholic extract 10 & 15 mg/kg Body wt. For 14 days by oral route afforded a significant protection against biochemical alterations induced by carbon tetrachloride. Thus the efficient protection of calves to varying degree, by the alcoholic extract of Picrorhiza kurrooa Benth given with carbon tetrachloride was observed from the clinical & biochemical investigations. [Vet. World 2010; 3(1.000: 29-31

  11. UMEDECIMENTO DO SUBSTRATO E TEMPERATURA NA GERMINAÇÃO DE SEMENTES DE Parkia platycephala BENTH.

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    Edilma Pereira Gonçalves

    2015-01-01

    Full Text Available The Parkia platycephala Benth. (faveira is a leguminous tree, known as a provider of commercial wood in the Amazon rain forest. This study aimed to evaluate the effects of different volumes of water for substrate moisture and temperatures on germination of Parkia platycephala . After this, they were sowing over towel paper, organized in rolls wetted with water contents equivalent to 2.0; 2.5; 3.0 and 3.5 times the weight of the substrate without new water addition, and they were maintained in chambers at constant temperatures of 20, 25, 30°C and alternate temperature 20-30°C. The following parameters were analyzed: the germination percentage, first count germination, index of germination speed, length and dry mass of seedlings (shoot and root. A completely randomized design was used with a 4 x 4 factorial (water volumes and temperatures. The moistening of the substrate with 2.0, 2.5, 3.0 and 3.5 times the weight of the paper at temperatures of 25, 30 and 20-30ºC can be used for germination and vigor of Parkia platycephala Benth. The temperature of 20ºC and all volumes of water tested for wetting the substrate reduced the germination and the vigor of these seeds.

  12. SABIA (Mimosa caesalpiniaefolia Benth. SEEDLINGS GROWTH AND SURVIVAL INOCULATED WITH SYMBIOTIC MICROORGANISMS IN FIELD CONDITIONS

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    Marília Malta Cavalcante Mendes

    2013-06-01

    Full Text Available http://dx.doi.org/10.5902/198050989277Sabia (Mimosa caesalpiniaefolia Benth. has ecological characteristics and symbiotic associations with nitrogen fixing bacteria and arbuscular mycorrhizal fungi. These associations are used as biological tool that could be used as an able alternative to minimize chemical fertilizer use and benefit plant development at environments under nutritional deficit. The objective of this study was to evaluate the growth of Leguminosae Sabia (Mimosa caesalpiniaefolia Benth. like response to rhizobium and arbuscular mycorrhizal fungi inoculation, to establish growth curves to height and stem based diameter, to determine the N and F contents in plants aerial parts and to evaluate plants survival at field. The strain used was BR3405, The arbuscular mycorrhizal fungi used was Glomus clarum and Gigaspora margarita. The Glomus clarum + Gigaspora margarita + BR3405 and Gigaspora margarita inoculation provided better values for the studied variables (height, diameter, N and P , and this last one was the best choice to Sabia plantation. The plants showed 100% survival, regardless the treatment ued. There was not significant statistical difference for N and F content in Sabia aerial part., Nevertheless, it was noticed a faster growth in plants with symbiont.

  13. GERMINAÇÃO DE SEMENTES DE Piptadenia moniliformis Benth. SOB ESTRESSE HÍDRICO

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    Gilvaneide Alves de Azerêdo

    2016-01-01

    Full Text Available The period of germination and the establishment of seedlings are important factors for the survival of species, mainly in places where availability of water is limited, as in the Caatinga region. In this sense, the purpose of this research was to evaluate the effect of water stress on the germination of Piptadenia moniliformis Benth seeds. Three lots (L 1 , L 2 e L 3 were used, corresponding to the production years of 2006, 2007 and 2008, respectively. Before the germination test, the seeds were submitted to scarification in concentrated sulfur acid for 30 minutes. To induce water deficit, polyethyleneglicol (PEG 6000 was used, in the following osmotic potentials: - 0,3; -0,6; -0,9, -1,2 and -1,5 MPa and water (0 MPa under the temperatures of 25 and 30 ºC. The features the experiment evaluated were: percentage of germination and of normal seedlings, germination speed index and the dry mass of seedlings. The germinating process of Piptadenia moniliformis Benth seeds is jeopardized from water potentials inferior to -0,6 MPa at 25 and 30 °C; water potentials equivalent or inferior to -1,2 MPa inhibit the formation of normal seedlings in both temperatures; the tolerance to water stress simulated with PEG 6000 is variable among seedling lots and germination temperatures.

  14. Characterisation of the anti-inflammatory and antinociceptive activities of the Hyptis pectinata (L.) Poit essential oil.

    Science.gov (United States)

    Raymundo, Larissa J R P; Guilhon, Carolina C; Alviano, Daniela S; Matheus, Maria Eline; Antoniolli, Angelo R; Cavalcanti, Sócrates C H; Alves, Péricles B; Alviano, Celuta S; Fernandes, Patrícia D

    2011-04-12

    Hyptis pectinata Poit (Lamiaceae) is grown in the northeastern regions of Brazil and is popularly known as "sambacaitá" or "canudinho". It is extensively used in folk medicine to treat inflammatory conditions, bacterial infections, pain, and cancer. Hyptis pectinata essential oil (EO, 10, 30, and 100mg/kg, p.o.) and the reference drugs morphine (5mg/kg, p.o.) and acetylsalicylic acid (ASA, 200mg/kg, p.o.) were evaluated using models for analgesia (acetic acid-induced contortions and hot plate) or inflammation (formalin-induced licking response and the subcutaneous air-pouch model). To elucidate the EO's mechanism of action, animals were pre-treated with the opioid receptor antagonist naloxone (1mg/kg, i.p.), the cholinergic antagonist atropine (1mg/kg, i.p.), or l-nitro arginine methyl ester (l-NAME, 3mg/kg, i.p.) 30 min prior to the oral administration of the EO. The EO significantly inhibited the number of writhings and the time the animals spent licking their formalin-injected paws (second phase). The EO, at doses of 30 and 100mg/kg, increased baseline measurements and area under the curve measurements in the hot plate model, respectively. The administration of naloxone reversed the antinociceptive effect of the EO in the hot plate model. l-NAME significantly reversed the effects of the EO in the contortions and hot plate models. Atropine completely reversed the antinociceptive activity of the EO in all models. Additionally, the EO inhibited the inflammatory process induced by subcutaneous carrageenan injection by reducing cell migration, exudate volume, protein concentration, and inflammatory mediators (nitric oxide, prostaglandin E2, IL-6, and TNF-α) produced in the pouch. Our results indicate that the Hyptis pectinata essential oil exhibits antinociceptive effects, likely mediated by opioid and cholinergic receptors, and anti-inflammatory activity through the inhibition of nitric oxide and PGE2 production. Copyright © 2011 Elsevier Ireland Ltd. All rights

  15. Physiological maturity of ingazeiro Inga striata Benth. SeedsMaturação fisiológica de sementes de ingazeiro (Inga striata Benth

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    Marlene Feliciano Mata

    2013-05-01

    Full Text Available Inga striata Benth., Popularly known as ingazeiro, is a native fruit of tropical forests and riparian forests of the Amazon, Northeast and Minas Gerais. It is used in the reclamation of degraded areas and in rural and urban forestry. The objective of this study was to determine the most appropriate indices for assessing the maturity and ideal harvest seeds I. striata Benth. Trees I. striata were in swamp forest of altitude on Campus II of the Federal University of Paraíba (UFPB, Areia-PB. Assessments of seed maturation were performed in six seasons fruit harvests after anthesis (95, 110, 125, 140, 155 and 170 DAA and two consecutive years of observations (two seasons. The experimental design was completely randomized following a factorial 2 x 6 (two periods of observation and six seasons crop of fruit. At each harvest were evaluated dimensions of fruits and seeds, water content and dry weight of seeds and their germination and vigor (first germination, speed of germination, length and mass of the primary root and shoot. The seed physiological maturity was reached at 146 to 166 days after flowering in the first year and 155 days in the second year, in which periods had the lowest water content, maximum dry matter accumulation and germination; The size, content water, dry mass of seed germination and the dry weight of the aerial part of the seedling, were the best parameters that determined the physiological maturity of seeds of I. striata. Inga striata Benth. é uma arbórea nativa de ocorrência nas florestas tropicais e matas ciliares, distribui-se nas Antilhas, Sul da América tropical e Bolívia. No Brasil ocorre na Amazônia e região Nordeste até Minas Gerais, sendo utilizada na recuperação de solos de áreas degradadas, como frutífera e na arborização urbana. O objetivo do Inga striata Benth., popularmente conhecida como ingazeiro, é uma frutífera nativa das florestas tropicais e matas ciliares da Amazônia, Nordeste e Minas

  16. How climate change might influence the potential distribution of weed, bushmint (Hyptis suaveolens)?

    Science.gov (United States)

    Padalia, Hitendra; Srivastava, Vivek; Kushwaha, S P S

    2015-04-01

    Invasive species and climate change are considered as the most serious global environmental threats. In this study, we investigated the influence of projected global climate change on the potential distribution of one of the world's most successful invader weed, bushmint (Hyptis suaveolens (L.) Poit.). We used spatial data on 20 environmental variables at a grid resolution of 5 km, and 564 presence records of bushmint from its native and introduced range. The climatic profiles of the native and invaded sites were analyzed in a multi-variate space in order to examine the differences in the position of climatic niches. Maximum Entropy (MaxEnt) model was used to predict the potential distribution of bushmint using presence records from entire range (invaded and native) along with 14 eco-physiologically relevant predictor variables. Subsequently, the trained MaxEnt model was fed with Hadley Centre Coupled Model (HadCM3) climate projections to predict potential distribution of bushmint by the year 2050 under A2a and B2a emission scenarios. MaxEnt predictions were very accurate with an Area Under Curve (AUC) value of 0.95. The results of Principal Component Analysis (PCA) indicated that climatic niche of bushmint on the invaded sites is not entirely similar to its climatic niche in the native range. A vast area spread between 34 ° 02' north and 28 ° 18' south latitudes in tropics was predicted climatically suitable for bushmint. West and middle Africa, tropical southeast Asia, and northern Australia were predicted at high invasion risk. Study indicates enlargement, retreat, or shift across bushmint's invasion range under the influence of climate change. Globally, bushmint's potential distribution might shrink in future with more shrinkage for A2a scenario than B2a. The study outcome has immense potential for undertaking effective preventive/control measures and long-term management strategies for regions/countries, which are at higher risk of bushmint's invasion.

  17. Bioactive compounds of Aspergillus terreus-F7, an endophytic fungus from Hyptis suaveolens (L.) Poit.

    Science.gov (United States)

    da Silva, Igor Pereira; Brissow, Elson; Kellner Filho, Luis Claudio; Senabio, Jaqueline; de Siqueira, Kátia Aparecida; Vandresen Filho, Samuel; Damasceno, Jaqueline Lopes; Mendes, Suzana Amorim; Tavares, Denise Crispim; Magalhães, Lizandra Guidi; Junior, Policarpo Ademar Sales; Januário, Ana Helena; Soares, Marcos Antônio

    2017-03-01

    The compounds terrein (1), butyrolactone I (2), and butyrolactone V (3) were isolated from the ethyl acetate extract (EtOAc) of the endophytic fungus Aspergillus terreus-F7 obtained from Hyptis suaveolens (L.) Poit. The extract and the compounds presented schistosomicidal activity against Schistosoma mansoni; at 100 µg/mL for EtOAc extract, 1297.3 µM for compound 1, 235.6 µM for compound 2, and 454.1 µM for compound 3, they killed 100% of the parasites after 72 h of treatment. Compounds 1, 2, and 3 exerted moderate leishmanicidal activity against Leishmania amazonensis (IC50 ranged from 23.7 to 78.6 µM). At 235.6 and 227.0 µM, compounds 2 and 3, respectively, scavenged 95.92 and 95.12% of the DPPH radical (2,2-diphenyl-1-picryl-hydrazyl), respectively. Regarding the cytotoxicity against the breast tumor cell lines MDA-MB-231 and MCF-7, compound 2 gave IC50 of 34.4 and 17.4 µM, respectively, while compound 3 afforded IC50 of 22.2 and 31.9 µM, respectively. At 117.6 µM, compound 2 inhibited the growth of and killed the pathogen Escherichia coli (ATCC 25922). Compounds 1, 2, and 3 displayed low toxicity against the normal line of human lung fibroblasts (GM07492A cells), with IC50 of 15.3 × 103, 3.4 × 103, and 5.8 × 103 µM, respectively. This is the first report on (i) the in vitro schistosomicidal and leishmanicidal activities of the EtOAc extract of A. terreus-F7 and compounds 1, 2, and 3; and (ii) the antitumor activity of compounds 2 and 3 against MDA-MB-231 and MCF-7 cells.

  18. Efeito do extrato de tiririca e bioestimulante no enraizamento de estacas de Hyptis marrubioides Epl.

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    Priscila Pereira Botrel

    2014-10-01

    Full Text Available Hyptis marrubioides Epl. (hortelã-do-campo, é uma planta medicinal pertencente à família Lamiaceae, conhecida pela presença de óleos voláteis. Verifica-se uma tendência na utilização de reguladores vegetais para o enraizamento de estacas quando há necessidade de acelerar a propagação vegetativa ou no caso de espécies que apresentam difícil enraizamento como é o caso da H. marrubioides. O objetivo do presente trabalho foi avaliar diferentes concentrações do extrato de tubérculos de tiririca (0,0; 25,0%; 50,0% e 100,0%e do Bioestimulante Radifarm® (0,0 mL e 2,5 mL. Foram conduzidos dois experimentos em delineamento experimental inteiramente casualizado (DIC, sendo oprimeiro, constituído por seis repetições por tratamento e cinco plantas por parcela e o segundo com cinco repetições e 10 plantas por parcela. Após 40 dias foram avaliados o comprimento de raízes(cm, biomassa seca deraíz (mg, número de nós, número de folhas, número de brotos, comprimento do broto (cm, biomassa seca da parte aérea, porcentagem de enraizamento e teor de clorofila.Verificou-se diferença significativa nos tratamentos com extrato de tiririca apenas para a variável biomassa seca da parte aérea, observando uma relação dose-dependente. O Bioestimulante Radifarm® proporcionou maior número de nós em estacas herbáceas de H. marrubioides (6,24 e teor de clorofila a (189,4. Em relação à biomassa seca de raiz, o bioestimulante promoveu um acúmulo médio de 24,4 mg.

  19. Evaluación de un sistema para la micorrización in vitro en plantas de mora de castilla (Rubus glaucus, Benth)

    OpenAIRE

    Víctor Manuel Núñez-Zarante; Gabriel Roveda-Hoyos; Marcela Franco-Correa; María Margarita Ramírez-Gómez; Urley Adrian Pérez-Moncada

    2012-01-01

    Evaluation of an in vitro mycorrhization system of blackberry plants (Rubus glaucus, Benth). Objective. Obtain an in vitromycorrhization system in autotrophic culture systems of blackberry plants (Rubus glaucus, Benth). Materials and methods. We usedspores and root fragments with vesicles of Arbuscular Mycorrhizal Fungus (AMF) Glomus sp (GEV02). We established an autotrophicculture system of blackberry plantlets comparing two methods of direct inoculation of the AMF. We measured the number of...

  20. Wound healing property of ethanolic extract of leaves of Hyptis suaveolens with supportive role of antioxidant enzymes.

    Science.gov (United States)

    Shirwaikar, Annie; Shenoy, Radhika; Udupa, A L; Udupa, S L; Shetty, Somashekar

    2003-03-01

    Ethanolic extract of leaves of Hyptis suaveolens was evaluated for its wound healing activity in ether-anaesthetized Wistar rats at two different doses (400 and 800 mg/kg) using incision, excision, and dead space wound model. Significant increase in skin breaking strength, granuloma breaking strength, wound contraction, hydroxyproline content and dry granuloma weight and decrease in epithelization period was observed. A supportive study made on granuloma tissue to estimate the levels of catalase and superoxide dismutase recorded a significant increase in the level of these antioxidant enzymes. Granuloma tissue was subjected to histopathological examination to determine the pattern of lay-down for collagen using Van Gieson and Masson Trichrome stains. Enhanced wound healing activity may be due to free radical scavenging action of the plant and enhanced level of antioxidant enzymes in granuloma tissue. Better collagenation may be because of improved antioxidant studies.

  1. GC-MS and FT-IR analysis of a coastal medicinal plant-Hyptis suaveolens (L. Poit

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    Joselin Joseph

    2016-05-01

    Full Text Available Objective: To investigate the bioactive components of a coastal medicinal plant, Hyptis suaveolens (L. Poit. (H. suaveolens leaves using fourier transform-infrared spectroscopy and gas chromatography-mass spectrometer (GC-MS. Methods: The chemical compositions of the ethanol extract of whole plant of H. suaveolens was investigated using PerkinElmer GC-MS, while the mass spectra of the compounds found in the extract was matched with the National Institute of Standard and Technology library. Results: The results of fourier transform-infrared spectroscopy analysis confirmed the presence of secondary alcohols, phenols, alkanes, alkynes, aromatics, nitro compounds and aliphatic compounds. GC-MS analysis of the ethanolic extract revealed the existence of 30 phytochemical compounds. 5,5-Dimethylimidazolidin-2,4-diamine (20.35% was found to be the major compound. Conclusions: The results of this study offer a platform to use H. suaveolens leaves as herbal alternative for various diseases.

  2. Histochemical and ultrastructural study of Caesalpinia peltophoroides Benth. (Leguminosae-Caesalpinoideae seeds Estudo histoquímico e ultraestrutural de sementes de Caesalpinia peltophoroides Benth. (Leguminosae-Caesalpinoideae

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    Viviana Borges Corte

    2009-10-01

    Full Text Available The objective of this work was to correlate data on light microscopy observations through histochemical analysis and polarized light techniques and investigations in transmission electron microscopy (TEM to characterize the reserve materials in C. peltophoroides Benth. (Leguminosae-Caesalpinoideae cotyledons, popularly known as "sibipiruna", a tropical tree species with wide distribution in Brazil. The cotyledon mesophyll, especially in the abaxial face, is rich in unsaturated neutral lipids contained in numerous lipid bodies dispersed in the cytoplasm. Proteins, more concentrated in the adaxial face of the cotyledons, occur in all the mesophyll and are stored in protein bodies containing globoids, with variable number and size, responsible for accumulation of mineral reserves. Calcium oxalate druses have distribution restricted to the cotyledons adaxial face and are associated with protein bodies. Starch, also distributed all over the cotyledon mesophyll, occurs in small amounts in plastids with developed lamellar system. Secretory cavities rich in phenolic compounds occur among procambial strands.Este trabalho procurou correlacionar dados de observações em microscopia de luz através de técnicas histoquímicas e de luz polarizada e investigações em microscopia eletrônica de transmissão para caracterizar os materiais de reserva em cotilédones de C. peltophoroides Benth. (Leguminosae-Caesalpinoideae, conhecida popularmente como sibipiruna, ou falso pau-brasil, uma espécie tropical com ampla distribuição no Brasil. O mesofilo cotiledonar, especialmente na face abaxial, apresenta-se rico em lipídios neutros, insaturados, contidos em numerosos corpos lipídicos dispersos no citoplasma. As proteínas, mais concentradas na face adaxial dos cotilédones, ocorrem em todo o mesofilo e são armazenadas em corpos proteicos contendo globoides, com número e tamanho variáveis, responsáveis pelo acúmulo de reservas minerais. Drusas de oxalato

  3. Distribuição de biomassa e nutrientes na aréa de Mimosa caesalpiniaefolia Benth Biomassa and nutrients distribution of Mimosa caesalpiniaefolia Benth

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    Orieudo Nunes Moura

    2006-12-01

    Full Text Available O trabalho foi realizado na Estação Experimental de Itambé, PE, para avaliar a distribuição da biomassa e nutrientes em povoamentos de Mimosa caesalpiniaefolia Benth. Foram estudados dois povoamentos, em solo e topografia similares com oito e 11 anos de idade, nos espaçamentos de 3,0 x 3,0 m e 4,5 x 4,5 m, respectivamente. A biomassa foi determinada para cada componente aéreo. As amostras de folhas, galhos, cascas e fuste foram coletadas e analisadas quimicamente. A biomassa total encontrada foi de 66,22 e 80,78 t/ha, nos povoamentos I e II, respectivamente. O material lenhoso (galhos e lenho representou 96,52 e 97,98% da biomassa total, nos povoamentos I e II. A distribuição de biomassa nos povoamentos I e II foi, respectivamente; de galhos (44,99 e 53,40%, fuste (51,53 e 44,58%, casca (2,39 e 1,40% e folhas (1,10 e 0,62%. A ordem de concentração de nutrientes nos dois povoamentos, em todos os componentes da parte aérea, de modo geral, obedeceu à seguinte ordem decrescente: nitrogênio > cálcio > potássio > magnésio > enxofre > fósforo.This work was carried out at Itambé Experimental Station, in the State of Pernambuco, Brazil, to study biomass and nutrient distribution in stands of Mimosa caesalpiniaefolia Benth. Two stands with similar soil and topographical conditions were studied, involving plants eight and eleven years old, at 3.0 x 3.0 m and 4.5 x 4.5 m spacing, respectively. The biomass of each tree component was determined. Samples of leaves, branches, bark and stem were collected and chemically analyzed. Total biomass was 66.22 and 80.78 t/ha in stands I and II, respectively. Biomass distribution in stands I and II was: branches (44.99 and 53.40%, wood (51.53 and 44.58%, bark (2.39 and 1.40% and leaves (1.10 and 0.62%. Nutrients of the components for both stands showed the following concentration trend: Nitrogen > Calcium > Potassium > Magnesium > Sulfur > Phosphorus.

  4. Morfologia e anatomia foliar de Bauhinia curvulha Benth. (Leguminosae-Caesalpinioideae Morphology and anatomy of Bauhinia curvula Benth. (Leguminosae-Caesalpinoideae

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    Maria Helena Rezende

    1994-07-01

    Full Text Available Foi realizado o estudo morfológico e anatômico da folha de Bauhinia cumula Benth., espécie de cerrado. As observações foram feitas em folhas adultas, tratadas segundo a metodologia usual utilizada em anatomia vegetal. Foi observado que a espécie apresenta as seguintes características: folhas bilobadas cuja incisão foliar chega próximo à região motora; um par de estipulas membranáceas; um par de nectários extraflorais, localizadaos na base do pulvino proximal; a venação possui padrão acródomo; o pecíolo, que possui dois pulvinos, um proximal e um distai, apresenta epiderme contendo grande quantidade de tricomas tecto res simples e glândulas; a lâmina foliar é anfiestomática com predomínio de estômatos dos tipos anomocítico e paracítico; a epiderme adaxial é monoestratificada, subpapilosa, desprovida de tricomas tectores simples e glândulas; a epiderme abaxial é subpapilosa, apresentando numerosos tricomas tectores simples e glândulas; e o mesofilo é formado predominantemente por parênquima paliçádico.A morphological and anatomical study of Bauhinia curvula Benth. leaf, a cerrado spe-cies, was perfomed. The observations, in grown up leaves, prepared according to the costumary methodology used in plant anatomy, showed the following characteristics of the species: bilobed leaves with foliar incision close to the motor region; one pair of membranaceous stipules; one pair of extrafloral nectaries located on the basis of the proximal pulvinus; acrodromous patterns of leaf venetion; the petiole, which has two pulvinus, one proximal and one distal, has an epidermis containing large quantities of simple tector trichomes and glands; the leaf blade, amphiestomatic, with predominance of anomocytic and paracytic stomata; the adaxial epidermis uniserial, sub papillose, without tector trichomes and glands; the abaxial epiderms, sub-papillose, presents a high number of simple trichomes and glands; and the mesophyll with a basic

  5. Dormancy and Germination of Castilla Blackberry Seeds (Rubus glaucus Benth / Latencia y Germinación de Semillas de Mora de Castilla (Rubus glaucus Benth

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    Díaz Diez Cipriano Arturo

    2013-08-01

    Full Text Available We categorized the dormancy and germination ofblackberry (Rubus glaucus Benth seeds from the GermplasmBank System for Food and Agriculture of the Colombian Nation. A tetrazolium test showed normal seedling production viability, but seed coat impermeability prevented imbibition, which is considered an expression of exogenous dormancy; however, this was released by immersing the seeds in 5.25% sodium hypochlorite for 16 to 21 hours. The treatment was applied to 10 accessions of Castilla blackberry, harvested during the dry and rainy seasons. The seeds were germinated under light and dark conditions. The highest germination counts were obtained with the dry-season-collected seeds incubated in darkness and with the rainy-season seeds incubated under light conditions. Differential germination responses were also independently determined by genotype, incubation conditions (light or darkness and collecting season. / Se categorizaron la latencia y la germinación ensemillas de mora de Castilla (Rubus glaucus Benth, provenientes del Sistema de Bancos de Germoplasma para la Alimentación y la Agricultura de la Nación Colombiana. La prueba del tetrazolio demostró que las semillas tenían la capacidad para dar origen a una plántula normal; sin embargo, al contacto con el agua la imbibición fue restringida por la impermeabilidad de la testa, lo que se considera como una expresión de latencia exógena. Ésta pudo ser removida con la inmersión de las semillas durante 16 a 21 horas en hipoclorito de sodio al 5,25%. El tratamiento fue aplicado a 10 accesiones de mora de Castila cosechadas en épocas seca y lluviosa; las semillas se germinaron bajo condiciones deluz y oscuridad, con una mayor germinación al incubar lasprovenientes de tiempo seco en condiciones de oscuridad y en aquellas de época lluviosa al ser germinadas con luz. También al aplicar el procedimiento de remoción de latencia exógena se observaron respuestas germinativas diferenciales

  6. Genotoxicity assessment of two common curing weeds: Hyptis suaveolens (L.) Poir. and Leucas indica (L.) R. Br.

    Science.gov (United States)

    Sumitha, K V; Thoppil, J E

    2016-08-01

    Hyptis suaveolens and Leucas indica, two common weeds were selected for the present study, to reveal their probable cytotoxic potential. The meristematic root tips of Allium cepa were used for testing the cytotoxic property of the aqueous leaf extracts containing both polar and non-polar compounds, and that containing polar compounds alone, at different concentrations (0.125, 0.25, 0.5, 1 and 2 %) and at different time durations, using distilled water as negative control. Mitotic squash preparations were made using a standard protocol. The mitotic index of the treated root tip cells was found to be decreasing and the abnormality percentage was found to be increasing with increase in extract concentration when compared with the control. Maximum cytotoxicity was observed in the extract containing both polar and non-polar compounds. Both the tested plants were found to be cytotoxic. The abnormalities noticed were of both clastogenic (nuclear lesions, nuclear fragmentation, etc.) and non-clastogenic (aberrant cell wall formation at cytokinesis, ball metaphase, etc.) types. Both plant extracts were found to significantly (P Hyptis and 1.58 % (R(2) = 0.757) for Leucas. The results were also analysed statistically by using analysis of variance followed by appropriate post hoc tests. These two weeds are aromatic plants comprising of essential oils that are volatile, natural complex compounds characterized by a strong odour and formed as secondary metabolites. In nature, essential oils play an important role in the protection of the plants as insecticides by reducing their appetite for such plants. When specifically targeted the concept of effectively exploiting these weeds for the formulation of herbal insecticides/pesticides may be possible in the near future.

  7. Gastroprotection of Suaveolol, Isolated from Hyptis suaveolens, against Ethanol-Induced Gastric Lesions in Wistar Rats: Role of Prostaglandins, Nitric Oxide and Sulfhydryls

    OpenAIRE

    Vera-Arzave, Carlos; Antonio, Leticia Cruz; Arrieta, Jesús; Cruz-Hernández, Gerardo; Velázquez-Méndez, Antonio Magdiel; Reyes-Ramírez, Adelfo; Sánchez-Mendoza, María Elena

    2012-01-01

    Hyptis suaveolens is a medicinal plant that is, according to traditional medicine, considered useful in the treatment of gastric ulcers. Although its gastroprotective activity was reported, the active compounds have not been identified. Therefore, the aim of the present study was to identify at least one active compound potentially responsible for the gastroprotective activity of H. suaveolens by using a bioassay guided study with an ethanol-induced g...

  8. Variação química do óleo essencial de Hyptis suaveolens (L.) Poit., sob condições de cultivo

    OpenAIRE

    Martins,Felipe Terra; Santos,Marcelo Henrique dos; Polo,Marcelo; Barbosa,Luiz Cláudio de Almeida

    2006-01-01

    This study was performed to establish the correlation between the growth conditions and essential oil composition of Hyptis suaveolens from Alfenas (MG), Brazil. The plants were grown in a greenhouse, four treatments were used and they were harvested at two different periods of time (60 and 135-day-old plants). The essential oil composition was determined by GC-MS analysis. The terpenes spathulenol, globulol, dehydroabietol, a-cadinol and beta-phellandrene were the major constituents found in...

  9. Peningkatan kadar patchouli alcohol pada minyak nilam (Pogostemon cablin Benth dengan metode distilasi fraksinasi vakum

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    Rahma Isfaroiny

    2012-02-01

    Full Text Available The quality of nilam (Pogostemon cablin Benth oil depend on it patchouli alcohol value. At there time nilam farmers just has produced nilam oil with patchouli alcohol value about 26–28%. To increase this value, fractional vacuum distillation had been done in this study. Nilam oil has been isolated from drying leaf of nilam plant. Patchouli alcohol in this oil was analyzed by gas chromatography, next the oil was distillated by fractional vacuum and the patchouli alcohol was determined from each fraction. This study showed that fractional vacuum distillation of nilam oil gives a higher patchouli alcohol value. The increasing was 23.3387%; from 35.022% at nilam oil before fractination vacuum distillation to 58.3515% at the third fraction from fractional vacuum distillation. In fact, the fourth fraction had the highest patchouli alcohol, but it had dark colour so isn’t fill the standard quality.

  10. Licanol, um novo flavanol, e outros constituintes de Licania macrophylla Benth

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    Fernando Antônio de Medeiros

    2012-01-01

    Full Text Available The phytochemical investigation of L. macrophylla Benth led to the isolation of a new flavanol named licanol: (--4'-O-methyl-epigallocatechin-3'-O-α-L-rhamnoside, along with nine known compounds, identified as: (--4'-O-methyl-epigallocatechin, pheophytin A, 13²-hydroxy-(13²-S-pheophytin A, pheophytin B, sitosterol, stigmasterol, sitosterol-β-O-glucoside, betulinic alcohol and oleanolic acid. The structures were established based on IR, HR-ESI-MS, and NMR spectrometric data analysis with the aid of 2D techniques. The methanolic extracts of leaves and stem bark as well as the compounds licanol, 13²-hidroxi-(13²-S-feofitina A, and betulinic alcohol demonstrated antimicrobial activity against several bacterial strains.

  11. Pyrolysis of Parinari polyandra Benth fruit shell for bio-oil production

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    Temitope E. Odetoye

    2014-09-01

    Full Text Available Non-conventional agricultural residues such as Parinari polyandra Benth fruit shell (PPBFS are potential sources of biomass feedstock that have not been investigated for bio oil production. In this study, PPBFS was pyrolyzed via an intermediate pyrolysis process for the production of bio oil. The bio oils were obtained using a fixed bed reactor within a temperature range of 375–550 oC and were characterized to determine their physicochemical properties. The most abundant organic compounds present were acetic acid, toluene, 2-cyclopenten-1-one, 2-furanmethanol, phenol, guaiacol and 2,6-dimethoxyphenol. The bio-oil produced at 550 oC possessed a higher quantity of desirable compounds than those produced at lower temperatures. The presence of acetic acids in the bio-oil suggested the need to upgrade the bio-oil before utilization as a fuel source.

  12. Studies on the efficacy of Bridelia ferruginea Benth bark extract for domestic wastewater treatment

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    O.M. Kolawole

    2007-08-01

    Full Text Available The efficacy of Bridelia ferruginea Benth bark extract in wastewater treatment was investigated. Chemical analysis found the bark to contain potassium, sodium, calcium, magnesium, zinc, manganese, iron and copper. Phytochemical tests revealed the bark to contain tannins, phlobatannins, saponins, alkaloids, and steroids. Comparative studies using varying concentrations (0.5, 1.0, 2.5 and 5.0 % w/v with alum and ferric chloride showed that the bark extract was effective in the clarification and sedimentation of total solids in the waste water sample. The optimum dose achieved was 2.5 % w/v with a minimum of 24 hours contact time. The total bacteria counts were reduced by 46 % after 24 hours when the extract was used whereas ferric chloride achieved 50 % reduction and alum achieved 55 % reduction under similar conditions. The feasibility of using the bark extract as an additional coagulant is therefore discussed.

  13. Nothoapiole and α -Asarone Rich Essential Oils from Himalayan Pleurospermum angelicoidesBenth

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    Chandra S. Mathela

    2015-06-01

    Full Text Available Pleurospermum angelicoides Benth. , a herb used in folk medicine, was collected from near Milam glacier of Uttarakhand. Different plant parts were investigated for their terpenoid compositions, antifungal, antibacterial and antioxidant potential. Nothoapiole (5-allyl-4,6,7-trimethoxy-benzo[1,3] dioxide 1 was observed as a single major constituent (87.3% in the root essential oil while α -asarone was the major constituent of leaf and flower essential oils (23.2%, 20.7% respectively. Monoterpenes viz. limonene, α-pinene, γ-terpinene and perilla aldehyde were other constituents of leaf and flower oils. All the essential oils exhibited broad range of antioxidant potential. The leaf essential oil showed significant antifungal activity against Candida albicans and Candida glabrata.

  14. The water extract of Coleus barbatus Benth decreases gastric secretion in rats

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    Lygia A. Fischman

    1991-01-01

    Full Text Available Coleus barbatus (Labiatae Benth is popularly used in Brazil "for the healing of liver and stomach diseases". The water extract (WE 1 to 10 g/Kg, p.o. of stem and leaves given to rats and mice did not induce signs of intoxication. Preveious treatment of mice with WE (1 g/kg, p.o. shortened the sleeping time induced by pentobarbital (50 mg/Kg, i.p. by 37 por cento, althoyugh the extract alone did not increase the spontaneous activity nor did it induce hyperexcitability. In mice WE (2 g/Kg, p.o. increased the intestinal transit of charcoal by 30 por cento, while reduced gastric secretions ion rats treated with WE (2g/Kg intraduodenal 3,9 ± 1.0 to 0.5 ± 0.2 ml/4h, respectively. The treatment also reduced the total acid secretion from 34.4 ± 11.0 to 2.7 ± 0.5 mEq/l and raisedgastric pH from 2.2 ± 0.3 to 6.5 ± 0.8. Treatment with WE (2g/Kg, p.o. protected against gastric ulcers induced by stress (5.3 ± 1.6 and 1.5 ± 0.5 ulcers/cm², but did nor protect against indonethacin induced ulcers. The results show that the water extract of C barbatus Benth produces mild stimulation of thecentral nervous system and increases intestinal movements. The extract also reduces gastric secretion indicating an antidyspeptic activity, and protects against gastric ulcers induced by stress.

  15. The micropropagation of Coleus blumei Benth. vitrocultures under different paraffin or silicon oil stratums

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    Dorina RADOVET-SALINSCHI

    2007-05-01

    Full Text Available In the framework of this experiment was the study of the reaction of Coleus blumei Benth. vitrocultures in hypoxic conditions, using vitroplantlets having 2 weeks from initiation whereupon them was applied an oil stratum: paraffin or silicon oil. The oil stratum was of 1 cm3, 2 cm3 or 5 cm3, the witness – lot vitroplantlets bare of oil. In the moment when the vitroplantlets surpassed the oil stratum was initiated a subculture bare of oil. After each 4 weeks the vitroplantlets was analyzed and was determined the assimilatory pigments in leafs. After the first 12 weeks witness lot grew up of the oil stratums, over 48 experimental weeks were subcultivated 3 times. Using a stratum of 1 cm3 of paraffin or silicon oil, after 24 of weeks in double stratum culture, the vitroplantlets surpassed the oil stratum, at this faze was no observed any eminent inhibitory reaction. Covering the vitrocultures with a stratum of 2 cm3 of paraffin oil, it was noted an inhibition of increase on a period of 32 of weeks, the content of assimilatory pigments being with 8.9% lower than that earmarked to the witness lot to 4 weeks from inoculation (vitroplantule bare of oil. In this case – in subculture - was observed an inhibitory reaction for 9 weeks. Covering the vitroplantlets with 5 cm3 of paraffin oil or silicon stratum was observed a prolongation of grow inhibition until 44 weeks – which determine a stronger inhibition in subcultures for 24 weeks. For inducing a long time grow inhibition on Coleus blumei Benth. vitrocultures the best solution is using paraffin or silicon oil in a 5 cm3 stratum of paraffin or silicon oil, the vitroplantlets reaction being similar.

  16. New species of Hymenoptera associated with galls on Calliandra brevipes Benth. (Fabaceae, Mimosoidea) in Brazil Novas espécies de Hymenoptera associadas a galhas de Calliandra brevipes Benth. (Fabaceae, Mimosoidea)

    OpenAIRE

    Angélica M. Penteado-Dias; Carvalho, Fabrício M. de

    2008-01-01

    Four species of Hymenoptera: Tanaostigmodes ringueleti (Brèthes, 1924), T. mecanga sp.nov. (Chalcidoidea, Tanaostigmatidae), Allorhogas taua sp. nov. (Braconidae, Doryctinae) and Eurytoma sp. (Chalcidoidea, Eurytomidae) were reared from two different types of galls of Calliandra brevipes Benth. (Fabaceae, Mimosoidea) in Juiz-de-Fora, Minas Gerais State, Brazil. The two Tanaostigmatidae species are probably the gall inducers; the Braconidae species probably is phytophagous inquiline in round g...

  17. Teor e composição química do óleo essencial de Hyptis marrubioides Epling (Lamiaceae em diferentes genótipos Essential oil content and chemical composition in Hyptis marrubioides Epl. of different genotypes

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    P.P. Botrel

    2009-01-01

    Full Text Available Hyptis marrubioides Epling (hortelã-do-campo é espécie de uso medicinal conhecida pelas suas atividades contra infecções gastrointestinais, infecções de pele, dores e câimbras. Objetivou-se com este trabalho, avaliar o teor e composição química do óleo essencial de H. marrubioides em diferentes genótipos (roxo e branco e partes da planta fresca (folhas, inflorescências e caule. Os genótipos estudados foram identificados através das inflorescências das plantas que apresentam coloração roxa e branca. O delineamento experimental foi inteiramente casualizado, contendo as três partes da planta de ambos os genótipos, perfazendo um fatorial 3x2, com quatro repetições. O óleo essencial foi extraído por hidrodestilação por duas horas e analisado por Cromatografia Gasosa acoplada a Espectrometria de Massas (CG-EM. O maior teor de óleo essencial foi observado na inflorescência do genótipo roxo. A composição do óleo essencial de H. marrubioides variou quantitativamente entre as partes das plantas analisadas e entre os genótipos. O composto α-tujona apresentou a maior porcentagem de área do pico nas inflorescências de genótipo roxo. As porcentagens das plantas com genótipo roxo foram em média superiores às plantas com genótipo branco.Hyptis marrubioides Epling ("hortelã-do-campo" is a medicinal species known for its activities against gastrointestinal and skin infections, pains and cramps. The aim of this work was to evaluate essential oil content and chemical composition in H. marrubioides of different genotypes (purple and white, as well as fresh plant parts (leaves, inflorescences and stem. The studied genotypes were identified based on the inflorescences, which are purple and white. The experimental design was completely randomized, including the three parts of both genotypes, in a 3X2 factorial arrangement, with four replicates. Essential oil was extracted through hydrodistillation for two hours and analyzed

  18. Proliferative effect of aqueous extract of Hyptis fructicosa on liver regeneration after partial hepatectomy in rats Efeito proliferativo do extrato aquoso da Hyptis fructicosa na regeneração hepática após hepatectomia parcial em ratos

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    Sônia Oliveira Lima

    2012-01-01

    Full Text Available PURPOSE: To evaluate the effect of aqueous extract of Hyptis fructicosa on hepatic regeneration after partial hepatectomy in rats. METHODS: Sixteen rats were divided in two groups: C (Control Group and HF (Whose rats received aqueous extract of Hyptis fructicosa during 4 days using the dose of 100 mg/kg/day. On the consecutive day of this treatment, the animals of both groups underwent hepatectomy of about 67% of liver. Twenty four hours later, they were sacrificed, and the remaining mass of liver was removed and prepared to be studied through the PCNA immunohistochemical technique. RESULTS: The liver regeneration index of HF group was 53.56 ± 18.91%, while in C group was 21.12 ± 8.29% (p=0.0003. CONCLUSION: These results show that the administration of aqueous extract of Hyptis fructicosa using the dose of 100mg/kg/day increased the hepatocyte proliferation in the group HF.OBJETIVO: Avaliar o efeito do extrato aquoso da Hyptis fructicosa sobre a regeneração hepática após hepatectomia parcial em ratos. MÉTODOS: Dezesseis ratos foram divididos em dois grupos: C (grupo controle e HF (ratos que receberam o extrato aquoso da Hyptis fructicosa durante quatro dias na dose de 100mg/kg/dia. No dia consecutivo deste tratamento, os animais de ambos os grupos foram submetidos a hepatectomia de aproximadamente 67% do fígado. Vinte e quatro horas depois, eles foram sacrificados, e que a massa restante do fígado foi retirado e preparado para ser estudado através da técnica de imuno-histoquímica PCNA. RESULTADOS: O índice de regeneração hepática do grupo HF foi 53,56 ± 18,91%, enquanto no grupo C foi de 21,12 ± 8,29% (p=0,0003. CONCLUSÃO: Estes resultados mostram que a administração do extrato aquoso da Hyptis fructicosa na dose de 100mg/kg/dia aumentou a proliferação de hepatócitos no grupo HF.

  19. Chitons (Mollusca: Polyplacophora) procured by the French Benthédi-expédition, 1977, and the Md 32-réunion-expédition, 1982, in the Southwestern Indian Ocean

    NARCIS (Netherlands)

    Kaas, P.

    1985-01-01

    The late Eugène Leloup examined samples from five stations of the Benthédi-Expédition, 1977, in the North of the Mozambique Channel, containing four species of chitons, two of which proved to be new to science. The material here discussed, coming from eighteen Benthédistations, proved to contain

  20. Caracterização e ontogenia dos tricomas glandulares de Ocimum selloi Benth. - Lamiaceae Characterization and ontogeny of the glandular trichomes of Ocimum selloi Benth. (Lamiaceae

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    Letícia de Almeida Gonçalves

    2010-12-01

    Full Text Available Ocimum selloi Benth. (Lamiaceae é uma espécie nativa da América do Sul e na medicina popular tem sido usada devido suas propriedades analgésica, anti-inflamatória e antiespasmódica. O objetivo do presente trabalho foi identificar os tipos de tricomas glandulares que ocorrem nos órgãos vegetativos e reprodutivos de O. selloi e determinar a ontogenia desses tricomas. Ramos laterais em início de formação, folhas totalmente expandidas, flores em diferentes estádios de diferenciação, amostras de caule e do eixo das inflorescências foram analisados em microscopias de luz e eletrônica de varredura. Tricomas glandulares do tipo peltado e capitado subséssil foram observados no caule, nas folhas, no eixo da inflorescência e na superfície adaxial das sépalas. Nas sépalas foi encontrado, além dos tricomas secretores peltados e capitados subsésseis, o tricoma glandular capitado pedunculado. A ontogenia inicia-se com a expansão de uma célula protodérmica que, de acordo com a seqüência de divisões periclinais e anticlinais (ora simétricas, ora assimétricas, dá origem aos tricomas. A diferenciação dos tricomas glandulares peltados e capitados não é sincrônica e ocorre muito cedo no desenvolvimento da folha, do caule e do eixo floral.Ocimum selloi Benth. (Lamiaceae is native to South America and in traditional medicine has been used due to its analgesic, anti-inflammatory, and antispasmodic properties. The aim of this study was to identify the types of glandular trichomes that occur on the vegetative and reproductive organs of O. selloi and to determine trichome ontogeny. Lateral branches at the initial formation phase, fully opened leaves, flowers at different differentiation stages, and stem and inflorescence axes were analyzed under light and scanning electron microscopy. Glandular trichomes of the peltate and subsessile capitate types were observed on the stem, leaves, inflorescence axis and the adaxial surface of the

  1. LICOR DE MORA DE CASTILLA (Rubus glaucus Benth CON DIFERENTES PORCENTAJES DE PULPA BLACKBERRY LIQUOR (Rubus glaucus Benth WITH DIFFERENT PULP PERCENTAGES

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    Álvaro Montoya Gómez

    2005-12-01

    Full Text Available La mora de Castilla (Rubus glaucus Benth es una fruta de interés comercial y altamente perecedera, que presenta deterioro debido a su fragilidad e inadecuado manejo poscosecha, cantidades apreciables de fruta son afectadas, básicamente en sus características físicas, siendo este producto apto para el procesamiento industrial. Veinte kg de mora fueron despulpados, homogenizados y caracterizada la pulpa, determinando; grados brix de 6,0; porcentaje de acidez de 2,91 % y densidad de 0,991 g/ml en promedio. Se formularon licores, con una participación de la pulpa del 25 %, 30 %, 35 %, 40 %, 45 %, 50 % y 55 % iniciando con 35 °Brix, para todos los casos en el mosto fermentable, ajustando las formulaciones con sacarosa; la levadura inoculada fue Saccharomyces cerevisiae Meyen ex E.C. Hansen en concentración del 0,2 %; la fermentación se llevó a cabo en reactores con capacidad de 2 litros, con desfogue de manguera de látex, el mosto fermentable fue dejado en los reactores durante 20 semanas, luego los licores fueron filtrados y caracterizados físico-químicamente, evaluando el porcentaje de alcohol por destilación, encontrando que el porcentaje de 40 % de participación de pulpa, fue el de mayor concentración con 8,36 % de volumen de alcohol en promedio. Los resultados estadísticos mostraron una diferencia significativa entre los porcentajes de participación de pulpa y su influencia sobre la producción de alcohol para los valores de 25 % y 30 % con respecto a los porcentajes 35 %, 40 %, 45 %, 50 % y 55 %. Un modelo matemático lineal apropiado para la producción de alcohol en función de la participación de pulpa fue obtenido. La prueba sensorial con 10 jueces, mostró que el licor preparado con 35 % de participación de pulpa, fue el de mayor aceptación.The blackberry (Rubus glaucus Benth is a fruit of commercial interest that is highly perishable, showing appreciable damage due to fragileness and inadequate pos-harvesting handling

  2. Fenologia, biologia floral e germinação de plantas aromáticas: Hyptis suaveolens (L.) Poit.(Lamiaceae) e Mansoa standleyi (Steyerm.) A. H. Gentry (Bignoniaceae) Museu Paraense Emilio Goeldi.

    OpenAIRE

    Lopes, Isaac Luiz Magalhães; JARDIM, Mário Augusto Gonçalves

    2008-01-01

    As plantas aromáticas produzem óleos essenciais empregados nas indústrias farmacêutica e alimentícia. Com isso, é necessário conhecer a biologia das espécies como perspectivas na produção de matéria prima vegetal. O objetivo desta pesquisa foi conhecer a fenologia e a biologia floral de Hyptis suaveolens (L.) Poit. (Lamiaceae) e Mansoa standleyi (Steyerm.) A. H. Gentry (Bignoniaceae) e o melhor tipo de substrato para germinação de Hyptis suaveolens. A fenologia e a biologia floral...

  3. Morfologia, taxonomia, filogenia, anatomia foliar e titoquímica de espécies do gênero Hyptis Jacq. (Labiatae) ocorrentes em em Goiás e Tocantins

    OpenAIRE

    Ferreira, Heleno Dias

    2010-01-01

    O gênero Hyptis, com 280 espécies, pertence à família Labiatae, subfamília Nepetoideae, tribo Ocimae e subtribo Hyptidinae. O gênero Hyptis foi dividido em 27 seções, sendo que 13 seções (Apodotes, Cephalohyptis, Cyanocephalus, Cyrta, Eriosphaeria, Pachyphyllae, Gymneia, Induratae, Mesosphaeria, Polydesmia, Pusilae, Trichosphaeria e Xylodontes) ocorrem em Goiás/Tocantins. Para coleta do material botânico, destinado aos estudos morfológicos, taxonômicos, anatômicos, fitoquímicos, distribuiç...

  4. Essential oils from Hyptis marrubioides, Aloysia gratissima and Cordia verbenacea reduce the progress of Asian soybean rust

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    André Costa da Silva

    2014-04-01

    Full Text Available The aim of this study was to evaluate the potential of essential oils derived from Hyptis marrubioides, Aloysia gratissima and Cordia verbenacea for controlling Asian soybean rust. The inhibitory activities of the essential oils (applied in a concentration range of 0.05 - 2% on the germination of Phakopsora pachyrhizi urediniospores were investigated using in vitro assays. The curative and eradicating properties of the oils (applied in a concentration range of 0.05 - 0.5% were studied under greenhouse conditions using the P. pachyrhizi-susceptible soybean cultivar MG/BR 46 (Conquista. Scanning electron microscopy was employed to investigate the effects of the essential oils on the morphology of the P. pachyrhizi urediniospores. The treatment with the essential oils at all concentrations tested led to the total inhibition of spore germination in vitro. The curative application of the essential oils reduced the disease severity, expressed as the area under the disease-progress curves, by 33 to 41%, whereas the commercial fungicide (pyraclostrobin + epoxyconazole employed as a positive control reduced the severity by 61%. The treatment of infected plants with the essential oils resulted in morphological alterations in the fungal structures that were similar to those produced by the commercial fungicide, namely, a shrivelling of the urediniospores, appressoria, germ tubes and paraphyses.

  5. Essential Oils of Hyptis pectinata Chemotypes: Isolation, Binary Mixtures and Acute Toxicity on Leaf-Cutting Ants.

    Science.gov (United States)

    Feitosa-Alcantara, Rosana B; Bacci, Leandro; Blank, Arie F; Alves, Péricles B; Silva, Indira Morgana de A; Soares, Caroline A; Sampaio, Taís S; Nogueira, Paulo Cesar de L; Arrigoni-Blank, Maria de Fátima

    2017-04-12

    Leaf-cutting ants are pests of great economic importance due to the damage they cause to agricultural and forest crops. The use of organosynthetic insecticides is the main form of control of these insects. In order to develop safer technology, the objective of this work was to evaluate the formicidal activity of the essential oils of two Hyptis pectinata genotypes (chemotypes) and their major compounds on the leaf-cutting ants Acromyrmex balzani Emery and Atta sexdens rubropilosa Forel. Bioassays of exposure pathways (contact and fumigation) and binary mixtures of the major compounds were performed. The major compounds identified in the essential oils of H. pectinata were β-caryophyllene, caryophyllene oxide and calamusenone. The essential oils of H. pectinata were toxic to the ants in both exposure pathways. Essential oils were more toxic than their major compounds alone. The chemotype calamusenone was more toxic to A. balzani in both exposure pathways. A. sexdens rubropilosa was more susceptible to the essential oil of the chemotype β-caryophyllene in both exposure pathways. In general, the binary mixtures of the major compounds resulted in additive effect of toxicity. The essential oils of H. pectinata is a raw material of great potential for the development of new insecticides.

  6. Absolute configuration and conformational analysis of brevipolides, bioactive 5,6-dihydro-α-pyrones from Hyptis brevipes.

    Science.gov (United States)

    Suárez-Ortiz, G Alejandra; Cerda-García-Rojas, Carlos M; Hernández-Rojas, Adriana; Pereda-Miranda, Rogelio

    2013-01-25

    The (6'S)-configuration of brevipolides A-J (1-10), isolated from Hyptis brevipes, was established by X-ray diffraction analysis of 9 in conjunction with Mosher's ester analysis of the tetrahydro derivative 11 obtained from both geometric isomers 8 and 9 as well as by chemical correlations. The structure of the new brevipolide J (10) was characterized through NMR and MS data as having the same 6-heptyl-5,6-dihydro-2H-pyran-2-one framework possessing the cyclopropane moiety of all brevipolides but substituted by an isoferuloyl group instead of the p-methoxycinnamoyl moiety found in 8 and 9. Conformational analysis of these cytotoxic 6-heptyl-5,6-dihydro-α-pyrones was carried out on compound 9 by application of a protocol based on comparison between experimental and DFT-calculated vicinal (1)H-(1)H NMR coupling constants. Molecular modeling was used to correlate minimum energy conformers and observed electronic circular dichroism transitions for the isomeric series of brevipolides. Compounds 7-10 exhibited moderate activity (ED(50) 0.3-8.0 μg/mL) against a variety of tumor cell lines.

  7. Complementarity of DFT Calculations, NMR Anisotropy, and ECD for the Configurational Analysis of Brevipolides K-O from Hyptis brevipes.

    Science.gov (United States)

    Suárez-Ortiz, G Alejandra; Cerda-García-Rojas, Carlos M; Fragoso-Serrano, Mabel; Pereda-Miranda, Rogelio

    2017-01-27

    Brevipolides K-O (1-5), five new cytotoxic 6-(6'-cinnamoyloxy-2',5'-epoxy-1'-hydroxyheptyl)-5,6-dihydro-2H-pyran-2-ones (IC50 values against six cancer cell lines, 1.7-10 μM), were purified by recycling HPLC from Hyptis brevipes. The structures, containing a distinctive tetrahydrofuran ring, were established by comprehensive quantum mechanical calculations and experimental spectroscopic analysis of their NMR and ECD data. Detailed analysis of the experimental NMR (1)H-(1)H vicinal coupling constants in comparison with the corresponding DFT-calculated values at the B3LYP/DGDZVP level confirmed the absolute configuration of 3 and revealed its conformational preferences, which were further strengthened by NOESY correlations. NMR anisotropy experiments by the application of Mosher's ester methodology and chemical correlations were also used to conclude that this novel brevipolide series (1-5) share the same absolute configuration corresponding to C-6(R), C-1'(S), C-2'(R), C-5'(S), and C-6'(S).

  8. Chemical Composition and Antibacterial Activity of Essential Oil Hyptis suaveolens (L. Poit. (Lamiaceae from the Venezuelan Plains

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    Nurby Ríos Tesch

    2015-04-01

    Full Text Available The essential oil (EO obtained from the leaves and flowers of Hyptis suaveolens (L. Poit. collected from Guasdualito, Apure State, Venezuela. Oil was obtained by hydrodistillation using a Clevenger trap. The oil was analyzed by gases chromatography-mass spectrometry (GC/MS. Thirty-five compounds were identified in the leaves (91.9% of the sample and thirty compounds in the flowers (97.0% of the sample, the major compounds: 1,8-cineole (19.1% leaves, flowers 13.3%, fenchone (18.5% leaves, flowers 16.1%, bicyclogermacrene (12.7% leaves, flowers 18.8%, D-germacrene (6.3% leaves, flowers 10.0%. The antibacterial activity was evaluated by the agar diffusion method with disks against Staphylococcus aureus ATCC 25923, Enterococcus faecalis ATCC 29212, Escherichia coli ATCC 25922, ATCC 23357 Klebsiella pneumoneae, Salmonella typhi CDC 57, Pseudomonas aeruginosa ATCC 27853. Both EO inhibited the development of E. coli, K. pneumoneae, and S. typhi, with a minimun inhibitory concentration (MIC ranging between 300 μL/mL and 450 μL/mL. These results represent a contribution to the study of the EO of H. suaveolens, besides it the first report on the antibacterial activity of the EO obtained from flowers of this specie.

  9. Capacidad antioxidante y contenido de fenoles totales de Hyptis spp., P. Heptaphyllum, T. Panamensis, T. Rhoifolia, y Ocotea sp.

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    Geovanna Tafurt García

    2015-05-01

    Full Text Available En este trabajo se evaluó la posible correlación entre las actividades antioxidantes, el contenido de fenoles totales (CFT y la composición química de Lamiaceae (H. conferta, H. dilatata, H. mutabilis, H. suaveolens, Burseraceae (P. heptaphyllum, T. rhoifoila, T. panamensis y Lauraceae (Ocotea sp.. Para los extractos etanólicos obtenidos por maceración en frio y evaporación a sequedad, la Capacidad Antioxidante Equivalente al Trolox o la Actividad Antioxidante Total (AAT, fueron determinadas mediante un ensayo colorimétrico con el catión radical ABTS, la Concentración Efectiva (EC50 fue evaluada con el radical DPPH, y el Contenido de Fenoles Totales (CFT, fue establecido mediante el método de Folin-Ciocalteu. Tanto la AAT como la EC50 estuvieron altamente correlacionados con el CFT. Las cortezas de T. rhoifolia y T. panamensis mostraron las capacidades antioxidantes más altas. Las Burseraceae spp. mostraron los TPC más altos y las Lamiaceae (Hyptis spp. mostraron los TPC más bajos.

  10. Bioassay-guided evaluation of antinociceptive properties and chemical variability of the essential oil of Hyptis fruticosa.

    Science.gov (United States)

    Franco, Clóvis R P; Antoniolli, Angelo R; Guimarães, Adriana G; Andrade, Daniela M; Jesus, Hugo C R; Alves, Péricles B; Bannet, Leonard Edward; Patrus, Ana Helena; Azevedo, Eduardo G; Queiroz, Dinalva B; Quintans-Júnior, Lucindo J; Botelho, Marco Antonio

    2011-11-01

    The composition of three samples of essential oil (EO) extracted from the leaves and flowers of Hyptis fruticosa (Lamiaceae) were investigated by GC/MS and GC-FID. The variability of the constituents and biological activity were evaluated in the oil samples. Acetic acid-induced abdominal constrictions and formalin-induced pain tests in mice were used for screening the antinociceptive activity. The possible antagonism of the essential oils or morphine (MOR) antinociceptive effects by pretreatment with naloxone, showed no influence on the antinociceptive action of the oils in the acetic acid-induced writhing test. All examined oil samples presented antinociceptive activity. The oil sample obtained from the leaves collected during the vegetative growth stage, near São Cristóvão at Sítio Tujubeba exhibited the highest effect. The same oil sample had a main percentage of 1,8-cineole (18.70%). Nevertheless, the oil obtained from flowers collected at the same location, showed a significant difference (p < 0.05) in the response intensity in the first phase of paw licking (100 mg/kg) possibly due to the higher contents of α-pinene (20.51%) and β-pinene (13.64%). The results provide evidence for the use of H. fruticosa by traditional medicine practitioners in the management of pain. Copyright © 2011 John Wiley & Sons, Ltd.

  11. Larvicidal and repellent activity of Hyptis suaveolens (Lamiaceae) essential oil against the mosquito Aedes albopictus Skuse (Diptera: Culicidae).

    Science.gov (United States)

    Conti, Barbara; Benelli, Giovanni; Flamini, Guido; Cioni, Pier Luigi; Profeti, Raffaele; Ceccarini, Lucia; Macchia, Mario; Canale, Angelo

    2012-05-01

    Lamiaceae have traditionally been used in developing countries for their insecticidal and repellent properties against several insect species. In our research, the essential oil (EO) extracted from fresh leaves of Hyptis suaveolens (Lamiaceae), and its main constituents were evaluated for larvicidal and repellent activity against the Asian tiger mosquito, Aedes albopictus Skuse (Diptera: Culicidae), currently the most invasive mosquito worldwide. H. suaveolens EO had insecticidal activity against A. albopictus larvae and mortality was dosage dependent. At the highest dosages of 450 and 400 ppm, there were no significant differences on larval mortality, as mortality ranged between 98.33% and 93.33%, respectively. At dosages ranging from 250 to 350 ppm, mortality rates were lower and not significantly different from each other. Terpinolene was found to be the most effective pure compound. Efficacy protection from H. suaveolens EO, at dosages ranging from 0.03748 to 0.7496 μg cm(-2) of skin, was evaluated during 150 min of observation. Results indicated that this EO had a significant repellent activity (RD(50) = 0.00035 μg cm(-2); RD(90) = 0.00048 μg cm(-2)), with differences in repellency rates, as a function of both concentration and observation time. Protection time ranged from 16 to 135 min. These results clearly evidenced that the larvicidal and repellent activity of H. suaveolens EO could be used for the development of new and safer products against A. albopictus.

  12. Mutagenicity induced by the hydroalcoholic extract of the medicinal plant Plathymenia reticulata Benth

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    A Della Torre

    2011-01-01

    Full Text Available Plathymenia reticulata Benth has an anti-inflammatory effect and is capable of neutralizing the neuromuscular blockade induced by Bothrops jararacussu or Crotalus durissus terrificus venoms, probably by precipitating venom proteins (an effect caused by plant tannins. The present study aimed to evaluate the mutagenic activity of P. reticulata by using the Salmonella mutagenicity assay (Ames test and the micronucleus test in CHO-K1 cells. P. reticulata extract concentrations of 2.84, 5.68, 11.37, and 19.90 mg/plate were assayed by the Ames test using TA97a, TA98, TA100 and TA102 bacterial strains, with (+S9 and without (-S9 metabolic activation. Concentrations of 5, 1.6 and 0.5 μg/mL of P. reticulata extract were used for the micronucleus test. P. reticulata extract was mutagenic to TA98 (-S9 and showed signs of mutagenic activity in TA97a and TA102 (both -S9 strains. Micronucleus test CBPI values showed that the endogenous metabolic system increased the number of viable cells when compared to the non-activated samples and the micronucleus frequency increased when the cells were treated in the absence of S9. We concluded that P. reticulata extract may present direct mutagenic properties.

  13. Triterpenoid saponins from Clinopodium chinense (Benth.) O. Kuntze and their biological activity.

    Science.gov (United States)

    Zhu, Yin-Di; Hong, Jing-Yi; Bao, Feng-Da; Xing, Na; Wang, Ling-Tian; Sun, Zhong-Hao; Luo, Yun; Jiang, Hai; Xu, Xu-Dong; Zhu, Nai-Liang; Wu, Hai-Feng; Sun, Gui-Bo; Yang, Jun-Shan

    2017-09-11

    Four new ursane-type triterpenoid saponins, clinopoursaponins A-D (1-4), six new oleanane-type triterpenoid saponins, clinopodiside VII-XII (5-10), as well as eight known triterpene analogues (11-18), were isolated from the aerial parts of Clinopodium chinense (Benth.) O. Kuntze. The structures of the new compounds were determined based on extensive spectral analyses, including 1D ( 1 H and 13 C) and 2D NMR experiments (COSY, NOESY, HSQC, 2D TOCSY, HSQC-TOCSY and HMBC), HR-ESI-MS and chemical methods. Compounds 1-18 were evaluated for their protective effects against anoxia/reoxygenation-induced apoptosis in H9c2 cells and cytotoxicities against murine mammary carcinoma cell line 4T1. Compounds 8, 9 and 18 exhibited significant protective effects, while compound 1 exhibited cytotoxic activity with IC 50 value of 7.4 μm compared to 7.6 μm for the positive control 10-hydroxycamptothecin.

  14. Carbohydrate metabolism in photosynthetic and nonphotosynthetic tissues of variegated leaves of Coleus blumei Benth

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    Madore, M.A. (Univ. of California, Riverside (USA))

    1990-06-01

    Mature, variegated leaves of Coleus blumei Benth. contained stachyose and other raffinose series sugars in both green, photosynthetic and white, nonphotosynthetic tissues. However, unlike the green tissues, white tissues had no detectable level of galactinol synthase activity and a low level of sucrose phosphate synthase indicating that stachyose and possibly sucrose present in white tissues may have originated in green tissues. Uptake of exogenously supplied ({sup 14}C)stachyose or ({sup 14}C)sucrose into either tissue type showed conventional kinetic profiles indicating combined operation of liner first-order and saturable systems. Autoradiographs of white discs showed no detectable minor vein labeling with ({sup 14}C)stachyose, but some degree of vein labeling with ({sup 14}C)sucrose. Autoradiographs of green discs showed substantial vein loading with either sugar. In both tissues, p-chloromercuribenzenesulfonic acid had no effect on the linear component of sucrose or stachyose uptake but inhibited the saturable component. Both tissues contained high levels of invertase, sucrose synthase and {alpha}-galactosidase and extensively metabolized exogenously supplied {sup 14}C-sugars. In green tissues, label from exogenous sugars was recovered as raffinose-series sugars. In white tissues, exogenous sugars were hydrolyzed and converted to amino acids and organic acids. The results indicate that variegated Coleus leaves may be useful for studies on both phloem loading and phloem unloading processes in stachyose-transporting species.

  15. (L. Benth. en bosques secundarios en Escárcega, Campeche, México

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    J. L. López-Torres

    2005-01-01

    Full Text Available El T´zalam Lysiloma latisiliquum (L. Benth. en la región de Escárcega, Campeche, México, es la especie más abundante y con mayor importancia económica en bosques secundarios derivados de selva mediana subperennifolia, después haber sido intervenida mediante el sistema rosa-tumba-quema. El objetivo de este trabajo es analizar el comportamiento del crecimiento en diámetro de esta especie, en un periodo de 20 años de desarrollo. A partir de la variable diámetro normal (d1.3, agrupada en categorías diamétricas, se estimaron los incrementos anuales medio, máximo y mínimo para cada categoría. Los resultados indican que en el escenario más ventajoso se requiere de 10.6 años para que los árboles pasen de categoría reserva a categoría aprovechable y, en promedio, es necesario de 21.5 años para alcanzar la misma condición. El incremento en diámetro fue, en promedio, de 0.511 cm y se clasifica como medio.

  16. Furano diterpenes from Pterodon pubescens Benth with selective in vitro anticancer activity for prostate cell line

    Energy Technology Data Exchange (ETDEWEB)

    Spindola, Humberto M.; Carvalho, Joao E. de; Ruiz, Ana Lucia T.G.; Rodrigues, Rodney A. F.; Denny, Carina; Sousa, Ilza M. de Oliveira; Foglio, Mary Ann [Universidade Estadual de Campinas (UNICAMP), SP (Brazil). Centro Pluridisciplinar de Pesquisas Quimicas, Biologicas e Agricolas (CPQBA)]. E-mail: foglioma@cpqba.unicamp.br; Tamashiro, Jorge Y. [Universidade Estadual de Campinas (UNICAMP), SP (Brazil). Inst. de Biologia

    2009-07-01

    Activity guided fractionation of Pterodon pubescens Benth. methylene chloride-soluble fraction afforded novel 6{alpha}-acetoxi 7{beta}-hydroxy-vouacapan 1 and four known diterpene furans 2, 3, 4, 5. The compounds were evaluated for in vitro cytotoxic activities against human normal cells and tumour cell lines UACC-62 (melanoma), MCF-7 (breast), NCI-H460 (lung, non-small cells), OVCAR-03 (ovarian), PC-3 (prostate), HT-29 (colon), 786-0 (renal), K562 (leukemia) and NCI-ADR/RES (ovarian expressing phenotype multiple drugs resistance). Results were expressed by three concentration dependent parameters GI{sub 50} (concentration that produces 50% growth inhibition), TGI (concentration that produces total growth inhibition or cytostatic effect) and LC{sub 50} (concentration that produces .50% growth, a cytotoxicity parameter). Also, in vitro cytotoxicity was evaluated against 3T3 cell line (mouse embryonic fibroblasts). Antiproliferative properties of compounds 1, 4 and 5 are herein reported for the first time. These compounds showed selectivity in a concentration-dependent way against human PC-3. Compound 1 demonstrated selectivity 26 fold more potent than the positive control, doxorubicin, for PC-3 (prostrate) cell line based on GI{sub 50} values, causing cytostatic effect (TGI value) at a concentration fifteen times less than positive control. Moreover comparison of 50% lethal concentration (LC{sub 50} value) with positive control (doxorubicin) suggested that compound 1 was less toxic. (author)

  17. Emergência e desenvolvimento inicial de sementes de Dimorphandra mollis Benth. em campo

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    M.F. SOUZA

    2016-03-01

    Full Text Available RESUMO Dimorphandra mollis Benth. (Leguminosae-Caesalpinioideae é uma espécie arbórea, encontrada no bioma Cerrado, utilizada na recuperação de áreas degradadas e na ornamentação. Dos frutos, extrai-se a rutina, um bioflavonoide utilizado na indústria farmacêutica, o qual atua na permeabilidade e na resistência dos vasos capilares. Esta pesquisa teve como objetivo verificar os efeitos do tipo de solo e de adubos sobre a emergência e o vigor da fava-d’anta semeada diretamente no campo. O delineamento experimental adotado foi em blocos ao acaso, em esquema fatorial 4 x 2 (quatro tipos de adubação e dois tipos de solo, com sete repetições de cinco sementes cada. Foram avaliadas as seguintes características: emergência, primeira contagem da emergência, índice de velocidade de emergência, altura e diâmetro da plântula. Não houve interação significativa entre os fatores, tipo de solo e adubos. Os melhores resultados para emergência e IVE são obtidos quando a semeadura é realizada em solo local.

  18. Organogenesis and plant regeneration of Arachis villosa Benth. (Leguminosae) through leaf culture.

    Science.gov (United States)

    Fontana, María Laura; Mroginski, Luis Amado; Rey, Hebe Yolanda

    2009-12-01

    With the aim of developing an efficient plant regeneration protocol, leaflet explants of three accessions of Arachis villosa Benth. (S2866, S2867 and L97) were cultured on basic Murashige and Skoog medium supplemented with different combinations of plant growth regulators: alpha-naphthalenacetic acid, indole-3-butyric acid, 6-benzylaminopurine, kinetin and thidiazuron. The accession L97 was the only one able to differentiate buds through indirect organogenesis. The most suitable combination for bud regeneration was the basic medium added with 13.62 microM thidiazuron and 4.44 microM 6-benzylaminopurine. These results show the important role of the genotype in morphogenetic responses and the organogenetic effect of thidiazuron in Arachis villosa accession L97. A thidiazuron lacking media (only 0.54 microM alpha-naphthalenacetic acid, 13.95 microM kinetin and 13.32 microM 6-benzylaminopurine were added) promoted the elongation of the regenerated buds. Adventitious rooting was achieved 90 days after the isolated shoots were transferred to a rooting medium containing 0.54 microM alpha-naphthalenacetic acid.

  19. Preparation and characterization of microcapsules of Pterodon pubescens Benth. by using natural polymers

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    Alexandre Espada Reinas

    2014-12-01

    Full Text Available An oleaginous fraction obtained from an alcohol extract of the fruit of Pterodon pubescensBenth. (FHPp was microencapsulated in polymeric systems. These systems were developed using a complex coacervation method and consisted of alginate/medium-molecular-weight chitosan (F1-MC, alginate/chitosan with greater than 75% deacetylation (F2-MC, and alginate/low-molecular-weight chitosan (F3-MC. These developed systems have the potential to both mask the taste of the extract, and to protect its constituents against possible chemical degradation. The influence of the formulation parameters and process were determined by chemical profiling and measurement of the microencapsulation efficiency of the oleaginous fraction, and by assessment of microcapsule morphology. The obtained formulations were slightly yellow, odorless, and had a pleasant taste. The average diameters of the microcapsules were 0.4679 µm (F2-MC, 0.5885 µm (F3-MC, and 0.9033 µm (F1-MC. The best formulation was F3-MC, with FHPp microencapsulation efficiency of 61.01 ± 2.00% and an in vitro release profile of 75.88 ± 0.45%; the content of vouacapans 3-4 was 99.49 ± 2.80%. The best model to describe the release kinetics for F1-MC and F3-MC was that proposed by Higuchi; however, F2-MC release displayed first-order kinetics; the release mechanism was of the supercase II type for all formulations.

  20. Micromorphological traits and essential oil contents of Micromeria kerneri Murb. and M. juliana (L.) Benth. (Lamiaceae).

    Science.gov (United States)

    Kremer, Dario; Dunkić, Valerija; Ruščić, Mirko; Matevski, Vlado; Ballian, Dalibor; Bogunić, Faruk; Eleftheriadou, Eleni; Stešević, Danijela; Kosalec, Ivan; Bezić, Nada; Stabentheiner, Edith

    2014-02-01

    The chemical composition of the essential oil (analysed by GC and GC-MS), the types and distribution of trichomes and pollen morphology (analysed by scanning electron microscopy) were investigated in two closely related species, Micromeria kerneri Murb. and Micromeria juliana (L.) Benth. (Lamiaceae) from Southeast Europe as a contribution to their taxonomy. The essential oil of M. kerneri was characterized by a high concentration of oxygenated sesquiterpenes, with caryophyllene-oxide as the major compound. Caryophyllene-oxide was also the major component of the essential oil of M. juliana from all localities, except from Mt Krivošije (Montenegro), where piperitone oxide was the major constituent. Non-glandular trichomes, peltate trichomes, and two types of capitate trichomes (type 1 composed of one basal epidermal cell, and one head cell with subcuticular space; type 2 composed of one basal epidermal cell, two stalk cells, and one head cell with subcuticular space) were observed on leaves, the calyx and on the stem. Pollen of both species had six apertures (hexacolpate) set in the equatorial pollen belt (zonocolpate) and showed medium reticulate ornamentation. Multivariate analysis (PCA and UPGMA) of essential oil components clearly separated the investigated M. kerneri and M. juliana populations, and confirmed the opinion that they are different taxa. On the other hand, micromorphological traits between these species were the same. Nevertheless, definitive conclusions about the taxonomic relationships among these species will require genetic analysis. Copyright © 2013 Elsevier Ltd. All rights reserved.

  1. Phytochemical constituents, antioxidant activity and toxicity potential of Phlomis olivieri Benth.

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    M.R. Delnavazi

    2016-04-01

    Full Text Available Background and objectives: Phlomis olivieri Benth. (Lamiaceae is a medicinal plant widely distributed in Iran. In the present study, we have investigated the phytochemical constituents, antioxidant activity and general toxicity potential of the aerial parts of this species. Methods: Silica gel (normal and reversed phases and Sephadex LH-20 column chromatographies were used for isolation of compounds from methanol-soluble portion (MSP of the total extract obtained from P. olivieri aerial parts. The structures of isolated compounds were elucidated using 1H-NMR, 13C-NMR and UV spectral analyses. Antioxidant activity and general toxicity potential of MSP were also evaluated in DPPH free radical-scavenging assay and brine shrimp lethality test (BSLT, respectively. Results: One caffeoylquinic acid derivative, chlorogenic acid (1, one iridoid glycoside, ipolamiide (2, two phenylethanoid glycosides, phlinoside C (3 and verbascoside (5, along with two flavonoids, isoquercetin (4 and naringenin (6 were isolated and identified from MSP. The MSP exhibited considerable antioxidant activity in DPPH method (IC50; 50.4 ± 4.6 µg/mL, compared to BHT (IC50; 18.7 ± 2.1 µg/mL, without any toxic effect in BSLT at the highest tested dose (1000 µg/mL. Conclusion: the results of the present study introduce P. olivieri as a medicinal plant with valuable biological and pharmacological potentials.

  2. Antitrypanosomal Activities and Mechanisms of Action of Novel Tetracyclic Iridoids from Morinda lucida Benth.

    Science.gov (United States)

    Kwofie, Kofi D; Tung, Nguyen Huu; Suzuki-Ohashi, Mitsuko; Amoa-Bosompem, Michael; Adegle, Richard; Sakyiamah, Maxwell M; Ayertey, Frederick; Owusu, Kofi Baffour-Awuah; Tuffour, Isaac; Atchoglo, Philip; Frempong, Kwadwo K; Anyan, William K; Uto, Takuhiro; Morinaga, Osamu; Yamashita, Taizo; Aboagye, Frederic; Appiah, Alfred A; Appiah-Opong, Regina; Nyarko, Alexander K; Yamaguchi, Yasuchika; Edoh, Dominic; Koram, Kwadwo A; Yamaoka, Shoji; Boakye, Daniel A; Ohta, Nobuo; Shoyama, Yukihiro; Ayi, Irene

    2016-06-01

    Trypanosoma brucei parasites are kinetoplastid protozoa that devastate the health and economic well-being of millions of people in Africa through the disease human African trypanosomiasis (HAT). New chemotherapy has been eagerly awaited due to severe side effects and the drug resistance issues plaguing current drugs. Recently, there has been an emphasis on the use of medicinal plants worldwide. Morinda lucida Benth. is a popular medicinal plant widely distributed in Africa, and several research groups have reported on the antiprotozoal activities of this plant. In this study, we identified three novel tetracyclic iridoids, molucidin, ML-2-3, and ML-F52, from the CHCl3 fraction of M. lucida leaves, which possess activity against the GUTat 3.1 strain of T. brucei brucei The 50% inhibitory concentrations (IC50) of molucidin, ML-2-3, and ML-F52 were 1.27 μM, 3.75 μM, and 0.43 μM, respectively. ML-2-3 and ML-F52 suppressed the expression of paraflagellum rod protein subunit 2, PFR-2, and caused cell cycle alteration, which preceded apoptosis induction in the bloodstream form of Trypanosoma parasites. Novel tetracyclic iridoids may be promising lead compounds for the development of new chemotherapies for African trypanosomal infections in humans and animals. Copyright © 2016 Kwofie et al.

  3. Seasonal variability of the essential oil of Hesperozygis ringens (Benth. Epling.

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    C. G. Pinheiro

    Full Text Available Abstract This study was developed to evaluate the effect of seasonality on the yield and chemical composition of the essential oil (EO of Hesperozygis ringens (Benth. Epling, a native species from the Brazilian Pampa. Leaves were collected from four specimens of a single population in each of the four seasons for a year and were extracted in triplicate by hydro-distillation for 2 hours. The yield of EO (% w/w was calculated on fresh weight basis (FWB, and the 16 oil samples were analyzed by gas chromatography coupled to mass spectrometry (GC-MS and gas chromatography with flame ionization detector (GC-FID. Hierarchical Cluster Analysis (HCA and Principal Component Analysis (PCA were used as statistical tools to evaluate differences in chemical composition. The highest yields were obtained in autumn, spring and summer (2.32-4.38%, while the lowest yields were detected in winter, ranging from 1.15 to 1.91%. Oxygenated monoterpenoids were the predominant class of chemical constituents in the EO obtained in all seasons, showing the highest contents in autumn and summer, and pulegone was identified as a major compound, whose contents varied between 54.13 and 81.17%. The EO samples were divided into three chemical groups by HCA and PCA and were assigned to the same group, except for the three samples gathered in winter. The results showed a seasonal influence on the yield and chemical composition of the EO.

  4. Ultrasound as pretreatment to convective drying of Andean blackberry (Rubus glaucus Benth).

    Science.gov (United States)

    Romero J, Carlos A; Yépez V, Byron D

    2015-01-01

    In this study, we evaluated the use of ultrasound as a pretreatment for convective drying of Andean blackberry (Rubus glaucus Benth). For this, a Box-Behnken experimental design was used to study the effect of ultrasound vibration amplitude (0-90μm), time of sonication (10-30min) and air temperature (40-60°C) on the retention of antioxidant compounds and on the kinetics of convective drying. The results showed that the antioxidant activity on fruit was reduced as the vibration amplitude and time of sonication increased, while was found that vibration amplitude ultrasound and air drying temperature were the variables that more affect the drying rate of blackberries. The drying rate increased by almost five times when samples were treated with ultrasound at 90μm for 20min. They were then dried using air at 60°C. It is concluded that the application of ultrasound in blackberry processing allows to obtain a dehydrated product with better functional quality and shows to be effective in reducing the time necessary to achieve a given value of moisture during convective drying. Copyright © 2014 Elsevier B.V. All rights reserved.

  5. Evaluation of the immunotoxicological effects of Dimorphandra mollis Benth., Fabaceae, in rats

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    Cássia A. O. Feres

    Full Text Available Dimorphandra mollis Benth., Fabaceae, also known as "faveira" or "fava-d'anta", is a plant common to the central woodsy meadow region of Brazil. It is well known for its antioxidant, antiplatelet and, principally, vasoprotective properties. Its principal component is rutin. The objective of this study is the evaluation of the safety of the use of the dried D. mollis extract in rodents. The rutin content of the standardized extract was 76.0±3%. With respect to the biochemical and hematological parameters evaluated, no alterations in the groups of rats that received 1000 and 2000 mg/kg doses of D. mollis were observed, but an increase in eosinophiles occurred. Hyperactivity of the white splenic pulp was detected in the group that received the 2000 mg/kg dose of D. mollis. In the evaluation of the lymphproliferative response with 1000 and 2000 mg/kg, no alterations were observed, and a decrease in IgG was only observed in the studies with a 2000 mg/kg dose. The results obtained with rodents suggest that no toxicity exists with the administration of dried D. mollis extract in a 1000 mg/kg dose.

  6. Pollination ecology of Acacia gerrardii Benth. (Fabaceae: Mimosoideae under extremely hot-dry conditions

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    Abdulaziz Saad Alqarni

    2017-11-01

    Full Text Available Talh trees (Acacia gerrardii Benth. are acacias that are native to the arid and semiarid Africa and west Asia. We investigated the flowering biology, pod set and flower visitors of Talh and discussed the role of these visitors in pollen transfer. The Talh trees blossomed laterally on the nodes of one-year-old twigs. Each node produced 21 flower buds seasonally. Each flower bud opened to a flower head (FH of 60 florets. The bagged FHs podded significantly (p ⩽ 0.05 less than did the unbagged FHs. The FHs were visited by 31 insect species (25 genera, 16 families and 5 orders. The major taxa were honeybees, megachilids, butterflies, ants, beetles and thrips. Each of honeybees, megachilids and beetles showed a significant (p ⩽ 0.05 hourly pattern, while each of butterflies, ants and thrips had no hourly pattern (p > 0.05. Furthermore, some birds and mammals touched the Talh FHs. Talh trees evolved a mass flowering behavior to face pre- and post-flowering obstacles. Megachilids seemed to play the major effort of zoophily because of their relatively high numbers of individuals and species and their effective movement behavior on the FH surface. Nevertheless, honeybees and other insects and vertebrate taxa also contributed to the pollen transfer. These results greatly contribute to our understanding of the pollination ecology of acacias, especially Arabian acacias.

  7. The Bioassay-Guided Isolation of Growth Inhibitors of Adult T-Cell Leukemia (ATL, from the Jamaican Plant Hyptis verticillata, and NMR Characterization of Hyptoside

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    Naomichi Arima

    2012-08-01

    Full Text Available Through bioassay-guided isolation, five compounds with growth inhibitory activity on S1T, an adult T-cell leukemia (ATL cell line, were isolated from the crude methanol extract of the aerial parts of Hyptis verticillata.

  8. Variação de terpenos em Hyptis suaveolens e seu papel na defesa contra herbívoros The role of terpene variation in Hyptis suaveolens in the defense against herbivores

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    Rachel Benetti Queiroz-Voltan

    1995-01-01

    Full Text Available Acompanhou-se o desenvolvimento de populações de Hyptis suaveolens (L. Poit. (Labiatae em três localidades paulistas: Horto Florestal de Sumaré, Câmpus da UNICAMP (Campinas e Fazenda Santa Genebra (Distrito de Barão Geraldo, Campinas, em 1981-83. Em cada local, escolheu-se uma população exposta à luz e outra, à sombra, com o objetivo de verificar em que fase do ciclo de vida as populações se tornavam mais suscetíveis ao ataque de herbívoros; de estimar a abundância dos herbívoros naturais e seus efeitos nas populações e a conseqüência da variação química dos monoterpenos que nelas ocorrem sobre os herbívoros. As populações tornavam-se mais suscetíveis aos herbívoros no período que antecedia a floração. Não se observou forte ataque de herbívoros nessa fase, embora se apresentassem em maior densidade. Os resultados sugerem que a variação na composição química possa afetar o desenvolvimento de herbívoros generalistas. No entanto, o Pyrausta insignatalis Guenée (Lep.-Pyralidae-Pyraustinae, provavelmente bem adaptado à planta, parece não ter sido afetado por esses terpenos. Não se detectaram diferenças entre populações expostas ao sol ou à sombra. Tais resultados apóiam a idéia de que a variabilidade química dentro de populações vegetais é importante estratégia de defesa contra herbívoros, dificultando-lhes a especialização.Populations of Hyptis suaveolens (L. Poit. (Labiatae were accompanied during their development in three sites: Horto Florestal de Sumaré, Campus of UNICAMP (Campinas, and Fazenda Santa Genebra (Distrito de Barão Geraldo, Campinas, all in the State of São Paulo, Brazil, from 1981 through 1983. At each site, a population exposed to full sunlight and another in the shade were studied. The objective was to evaluate, during plant development, the period when they were more susceptible to attack by herbivores; to consider the abundance of herbivores, and the role of variation

  9. Palinotaxonomia de representantes das tribos Coussareeae. Benth. Hook. F. e Psychotrieae Cham. & Schtdl (Rubiaceae Juss.) ocorrentes no Sudeste do Brasil

    OpenAIRE

    Gabrielle Reboredo Menezes Vieira

    2015-01-01

    Rubiaceae Juss. é a quarta família em número de espécies entre as Angiospermas no Mundo e no Neotrópico, apresentando grande diversidade de espécies com representantes na maioria dos biomas. No Brasil são registrados 124 gêneros e 1.395 espécies sendo considerada uma das principais famílias da flora brasileira. O presente trabalho trata do estudo palinológico de 61 espécies da família Rubiaceae subordinadas às tribos Coussareeae Benth. & Hook.f e Psychotrieae Cham. & Schltdl. o...

  10. GERMINAÇÃO DE SEMENTES DE Hyptis suaveolens (L. POIT. (LAMIACEA EM FUNÇÃO DA LUZ E DA TEMPERATURA

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    Sandra Sely Silveira Maia

    2008-01-01

    Full Text Available This work aimed to evaluate the influence of light and temperature on germination of Hyptis suaveolens seeds. The seeds were harvested at Mossoró/RN city and the experiment developed in at the Laboratory of Seed Analysis of the Department of Agriculture of the Federal University of Lavras at Lavras/MG. The treatments was consisted of four temperatures (20ºC, 25ºC, 30ºC and 20-30ºC and two light conditions (light and darkness. The treatments consisted of 200 seeds, with four repetitions of 50 seeds each, sown on a two Germitest towel paper, displayed inside gerbox, and maintained for 30 days in germination chamber. A completely randomized constituted design with 2x4 factorial design (two light expositions x four temperatures was used with 4 replicates of 50 seeds. The Scott-Knot¿s Test at 5% of probability was used to compare the obtained averages. There was significant difference in the interaction temperature x shines, in the two variables. It can be concluded that the largest germination of seeds happened in the light presence and in high temperatures. And that the temperatures alternated of 20-30ºC, 30ºC and 25ºC are favorable for germination of seeds of Hyptis suaveolens.

  11. New species of Hymenoptera associated with galls on Calliandra brevipes Benth. (Fabaceae, Mimosoidea in Brazil Novas espécies de Hymenoptera associadas a galhas de Calliandra brevipes Benth. (Fabaceae, Mimosoidea

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    Angélica M. Penteado-Dias

    2008-09-01

    Full Text Available Four species of Hymenoptera: Tanaostigmodes ringueleti (Brèthes, 1924, T. mecanga sp.nov. (Chalcidoidea, Tanaostigmatidae, Allorhogas taua sp. nov. (Braconidae, Doryctinae and Eurytoma sp. (Chalcidoidea, Eurytomidae were reared from two different types of galls of Calliandra brevipes Benth. (Fabaceae, Mimosoidea in Juiz-de-Fora, Minas Gerais State, Brazil. The two Tanaostigmatidae species are probably the gall inducers; the Braconidae species probably is phytophagous inquiline in round gall type. The two new species are described and illustrated, including their immature stages.Quatro espécies de Hymenoptera: Tanaostigmodes ringueleti (Brèthes, 1924, T. mecanga sp. nov. (Chalcidoidea, Tanaostigmatidae, Allorhogas taua sp. nov. (Braconidae, Doryctinae e Eurytoma sp. foram obtidas de dois diferentes tipos de galhas de Calliandra brevipes Benth. (Fabaceae, Mimosoidea em Juiz-de-Fora, MG, Brasil. As duas espécies de Tanaostigmatidae são os prováveis indutores das galhas; a espécie de Braconidae, provavelmente é um inquilino fitófago das galhas globosas. Estas duas novas espécies são descritas e ilustradas, incluindo os seus estágios imaturos.

  12. Perennial peanut (Arachis glabrata Benth.) leaves contain hydroxycinnamoyl-CoA:tartaric acid hydroxycinnamoyl transferase activity and accumulate hydroxycinnamoyl-tartaric acid esters

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    Many plants accumulate hydroxycinnamoyl esters to protect against abiotic and biotic stresses. Caffeoyl esters, in particular, can be substrates for endogenous polyphenol oxidases (PPOs). Recently, we showed that perennial peanut (Arachis glabrata Benth.) leaves contain PPO and identified one PPO su...

  13. Labellar anatomy and secretion in Bulbophyllum Thouars (Orchidaceae: Bulbophyllinae) sect. Racemosae Benth. & Hook. f.

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    Davies, Kevin L; Stpiczyńska, Malgorzata

    2014-10-01

    Floral secretions are common in Bulbophyllum Thouars, and the labella of a number of Asian species are said to produce secretions rich in lipids that act as food rewards for insect pollinators. Although some of these reports are based on simple histochemical tests, a much greater number are anecdotal and, hitherto, neither the ultrastructure of the labellum nor the secretory process has been investigated in detail. Furthermore, sophisticated histochemical approaches have generally not been applied. Here, both the labellar structure and the secretory process are investigated for four species of Asian Bulbophyllum sect. Racemosae Benth. & Hook. f., namely Bulbophyllum careyanum (Hook.) Spreng., B. morphologorum Kraenzl., B. orientale Seidenf. and B. wangkaense Seidenf., and compared with those of unequivocal lipid-secreting orchids. Labellar, secretory tissue was investigated using light microscopy, scanning electron microscopy, transmission electron microscopy and histochemistry. The adaxial median longitudinal groove of the labellum contained secretory tissue comprising palisade-like epidermal cells, similar to those of certain lipid-secreting Oncidiinae Benth. However, these cells and their secretions gave positive results mainly for protein and mucilage, and their organelle complement was consistent with that of cells involved in protein and mucilage synthesis. Sub-cuticular accumulation of secretion resulted in cuticular distension and blistering. The sub-epidermal layer of isodiametric parenchyma contained starch and, like the epidermal cells, ultrastructure consistent with mucilage synthesis. Lipids were mainly confined to the cuticle, and hardly any intracellular lipid droplets were observed. It is proposed that mucilage is produced by dictyosomes present in the palisade-like epidermal cells. Mucilage precursors may also be produced by these same organelles in sub-epidermal cells and are thought to pass along the symplast via plasmodesmata into the adjoining

  14. Differential proteomics of Picrorhiza kurrooa Royle ex Benth. in response to dark stress.

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    Parkash, Jai; Kashyap, Sanjeeta; Kalita, Prakash Jyoti; Devi, Manjula; Ahuja, Paramvir Singh; Dutt, Som

    2014-09-01

    Picrorhiza (Picrorhiza kurrooa Royle ex Benth.) an important medicinal herb of western Himalayan region has been used to treat various diseases and disorders. Over-harvesting and lack of cultivation has led to its entry in Red Data Book as an endangered species. Further, its very restrictive habitat and lesser biomass production are major limitations for bringing it under commercial cultivation. All these issues necessitate deeper insights into mechanisms governing its growth and interaction with the environmental cues. Light may be one of the important factors to be studied for its role in regulating growth and adaptation of Picrorhiza as in natural habitat it prefers shady niches. Keeping this in view, proteome of Picrorhiza kept under light vis-à-vis under dark was analysed and compared. Leaf as well as root proteome of Picrorhiza was studied. Denaturing two dimensional gel electrophoresis and mass spectrometry techniques were used to detect and identify differentially expressed proteins, respectively. Twenty two proteins from leaf and 25 proteins from root showed differential expression levels under dark and light conditions. Among the differentially expressed proteins, majority were those involved in metabolism, protein synthesis, and stress and defense response. Other differentially expressed proteins were those involved in photosynthetic process, photorespiration and few proteins were with unknown function indicating that many different processes work together to establish a new cellular homeostasis in response to dark and light conditions. Proteins found to be differentially expressed under light vis-à-vis dark conditions suggested a range of biochemical pathways and processes being associated with response of plant to dark conditions. The identified proteins may be utilized for developing strategies for improving the biomass production/performance of Picrorhiza under varied light/dark habitats.

  15. Chemical Composition and Allelopathic Potential of Essential Oils from Tipuana tipu (Benth.) Kuntze Cultivated in Tunisia.

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    El Ayeb-Zakhama, Asma; Sakka-Rouis, Lamia; Bergaoui, Afifa; Flamini, Guido; Jannet, Hichem Ben; Harzallah-Skhiri, Fethia

    2016-03-01

    In Tunisia, Tipuana tipu (Benth.) Kuntze is an exotic tree, which was introduced many years ago and planted as ornamental street, garden, and park tree. The present work reported, for the first time, the chemical composition and evaluates the allelopathic effect of the hydrodistilled essential oils of the different parts of this tree, viz., roots, stems, leaves, flowers, and pods gathered in the area of Sousse, a coastal region, in the East of Tunisia. In total, 86 compounds representing 89.9 - 94.9% of the whole oil composition, were identified in these oils by GC-FID and GC/MS analyses. The root essential oil was clearly distinguished for its high content in sesquiterpene hydrocarbons (β-caryophyllene, 1 (44); 24.1% and germacrene D, 2 (53); 20.0%), while those obtained from pods, leaves, stems, and flowers were dominated by non-terpene hydrocarbons. The most important ones were n-tetradecane (41, 16.3%, pod oil), 1,7-dimethylnaphthalene (43, 15.6%, leaf oil), and n-octadecane (77, 13.1%, stem oil). The leaf oil was rich in the apocarotene (E)-β-ionone (4 (54); 33.8%), and the oil obtained from flowers was characterized by hexahydrofarnesylacetone (5 (81); 19.9%) and methyl hexadecanoate (83, 10.2%). Principal component and hierarchical cluster analyses separated the five essential oils into three groups and two subgroups, each characterized by the major oil constituents. Contact tests showed that the germination of lettuce seeds was totally inhibited by the root essential oil tested at 1 mg/ml. The inhibitory effect on the shoot and root elongation varied from -1.6% to -32.4%, and from -2.5% to -64.4%, respectively. © 2016 Verlag Helvetica Chimica Acta AG, Zürich.

  16. Antifungal Screening of Bridelia ferruginea Benth (Euphorbiaceae Stem Bark Extract in Mouthwash Formulations

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    Aremu Olusola Isaac

    2017-06-01

    Full Text Available The plant Bridelia ferruginea Benth (Euphorbiaceae has been known for its use in the management of oral thrush ethnomedicinally in various parts of Africa, a practice which has been justified by results of certain scientific studies. The aim of this study was to develop an appropriate dosage formulation, a mouthwash and evaluate the antifungal potential of this dosage formulation against a major causative organism of oral thrush, Candida albicans. Extraction of the stem bark was carried out with boiled distilled water, the extract was formulated into mouthwashes at concentrations of 0.5, 1.0, 1.5, 2.0 and 2.5%w/v. All formulations contained viscosity imparting agent, a sweetener and a preservative. Physical characterisation, viscosity, pH and palatability of the mouthwash formulations were determined. Agar-well diffusion method was used to assess antifungal activity of the formulations against Candida albicans and Nystatin oral suspension was used as reference compound. The results showed that Bridelia ferruginea stem bark extract mouthwash solutions were brown in colour, had agreeable odour and sweet astringent taste. The pH for all concentrations was in the range 5.41-5.63. The viscosity at spindle no 2, 60rpm range between 0.226-0.238 Pa.S for all concentrations studied. The formulations had antifungal activity against Candida albicans. The highest concentration (2.5%w/v gave mean zone of inhibition of 25.50±0.71mm that was comparable with Nystatin oral suspension 28.00±1.41mm, a reference compound. The foregoing suggests that with little modification in the formulation especially the adjustment of the pH, Bridellia ferruginea mouthwash solutions may be developed into commercially useful preparations.

  17. Gastroprotective effect of a flavone from Lonchocarpus araripensis Benth. (Leguminosae) and the possible mechanism.

    Science.gov (United States)

    Campos, Deive A; de Lima, Almi F; Ribeiro, Saulo Rodrigo L; Silveira, Edilberto R; Pessoa, Otilia Deusdênia L; Rao, Vietla S; Santos, Flávia A

    2008-03-01

    The gastroprotective effect of DDF (3,6-dimethoxy-6'', 6''-dimethyl-[2'', 3'' : 7,8]-chromeneflavone) from Lonchocarpus araripensis Benth. (Leguminosae) on gastric damage induced by absolute ethanol (96%, 0.2 mL/mouse) and indometacin (30 mg kg(-1), p.o.) in mice was investigated. Intraperitoneally administered DDF at dose levels of 50, 100 and 200 mg kg(-1) markedly reduced the gastric lesions in the ethanol model by 62, 72 and 96%, and in the indometacin model by 34, 70 and 75%, respectively, as compared with misoprostol (50 microg kg(-1), p.o.), the reference compound that caused lesion suppression by 67% in ethanol model and by 72% against indometacin-induced ulceration. The ED50 of DDF in reducing gastric lesions induced by ethanol and indometacin (dose of the DDF that reduced the gastric lesion area by 50% in relation to the control value) was 50.87 and 61.56 mg kg(-1), respectively. Mechanistic studies were carried out at 100 mg kg(-1) DDF using the ethanol model. Compared with N-acetylcysteine (750 mg kg(-1), p.o.), a donor of sulfhydryls, DDF only partially replenished the ethanol-induced depletion of gastric mucosal NP-SH. Pretreatment with TRPV1 antagonist capsazepine (5 mg kg(-1), i.p.) or the non-selective cyclooxygenase inhibitor indometacin (10 mg kg(-1), p.o.) effectively blocked the gastroprotective effect of DDF (100 mg kg(-1)) against ethanol damage. Furthermore, the effect of DDF was significantly reduced in mice pretreated with L-NAME, or glibenclamide, the respective inhibitors of nitric oxide synthase and K+ ATP channel activation. These data provide evidence to show that DDF affords gastroprotection against gastric damage induced by ethanol and indometacin by different and complementary mechanisms, which include involvement of endogenous prostaglandins, nitric oxide release, the activation of TRPV1 receptor or K+ ATP channels, besides a sparing effect on NP-SH reserve.

  18. Laxative activities of Mareya micrantha (Benth. Müll. Arg. (Euphorbiaceae leaf aqueous extract in rats

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    Djaman Joseph A

    2010-02-01

    Full Text Available Abstract Background Mareya micrantha (Benth. Müll. Arg. (Euphorbiaceae is a shrub that is commonly used in Côte d'Ivoire (West Africa for the treatment of constipation and as an ocytocic drug. The present study was carried out to investigate the laxative activity of Mareya micrantha in albino's Wistar rats. Methods Rats were divided in 5 groups of 5 animals each, first group as control, second group served as standard (sodium picosulfate while group 3, 4 and 5 were treated with leaf aqueous extract of Mareya micrantha at doses of 100, 200 and 400 mg/kg body weight (b.w., per os respectively. The laxative activity was determined based on the weight of the faeces matter. The effects of the leaves aqueous extract of Mareya micrantha and castor oil were also evaluated on intestinal transit, intestinal fluid accumulation and ions secretion. Results Phytochemicals screening of the extract revealed the presence of flavonoids, alkaloids, tannins, polyphenols, sterols and polyterpenes. The aqueous extract of Mareya micrantha applied orally (100, 200 and 400 mg/kg; p.o., produced significant laxative activity and reduced loperamide induced constipation in dose dependant manner. The effect of the extract at 200 and 400 mg/kg (p.o. was similar to that of reference drug sodium picosulfate (5 mg/kg, p.o. The same doses of the extract (200 and 400 mg/kg, p.o. produced a significant increase (p -, Na+, K+ and Ca2+ in the intestinal fluid (p Conclusions The results showed that the aqueous extract of Mareya micrantha has a significant laxative activity and supports its traditional use in herbal medicine.

  19. Antiplasmodial activity of sesquiterpene lactones and a sucrose ester from Vernonia guineensis Benth. (Asteraceae)

    Science.gov (United States)

    Toyang, Ngeh J.; Krause, Michael A.; Fairhurst, Rick M.; Tane, Pierre; Bryant, Joseph; Verpoorte, Rob

    2013-01-01

    Ethnopharmacological relevance Aqueous preparations of Vernonia guineensis Benth. (Asteraceae) are used in Cameroonian folk medicine as a general stimulant and to treat various illnesses and conditions including malaria, bacterial infections and helminthic infestations. Materials and methods 10-g samples of the leaf and tuber powders of V. guineensis were extracted separately using dichloromethane, methanol and distilled water. The extracts were dried in vacuo and used in bioassays. These extracts and three compounds previously isolated from V. guineensis [vernopicrin (1), vernomelitensin (2) and pentaisovalerylsucrose (3)] were screened for antiplasmodial activity against chloroquine (CQ)-sensitive (Hb3) and CQ-resistant (Dd2) Plasmodium falciparum lines. Results Crude extracts and pure compounds from V. guineensis showed antiplasmodial activity against both Hb3 and Dd2. The IC50 values of extracts ranged from 1.64 – 27.2 μg/ml for Hb3 and 1.82 – 30.0 μg/ml for Dd2; those for compounds 1, 2 and 3 ranged from 0.47 – 1.62 μg/ml (1364 – 1774 nM) for Hb3 and 0.57 – 1.50 μg/ml (1644 – 2332 nM) for Dd2. None of the crude extracts or pure compounds was observed to exert toxic effects on the erythrocytes used to cultivate the P. falciparum lines. Conclusion In Cameroonian folk medicine, V. guineensis may be used to treat malaria in part due to the antiplasmodial activity of sesquiterpene lactones (1, 2), a sucrose ester (3) and perhaps other compounds present in crude plant extracts. Exploring the safety and antiplasmodial efficacy of these compounds in vivo requires further study. PMID:23542146

  20. Antiplasmodial activity of sesquiterpene lactones and a sucrose ester from Vernonia guineensis Benth. (Asteraceae).

    Science.gov (United States)

    Toyang, Ngeh J; Krause, Michael A; Fairhurst, Rick M; Tane, Pierre; Bryant, Joseph; Verpoorte, Rob

    2013-06-03

    Aqueous preparations of Vernonia guineensis Benth. (Asteraceae) are used in Cameroonian folk medicine as a general stimulant and to treat various illnesses and conditions including malaria, bacterial infections and helminthic infestations. Ten gram samples of the leaf and tuber powders of Vernonia guineensis were extracted separately using dichloromethane, methanol and distilled water. The extracts were dried in vacuo and used in bioassays. These extracts and three compounds previously isolated from Vernonia guineensis [vernopicrin (1), vernomelitensin (2) and pentaisovalerylsucrose (3)] were screened for antiplasmodial activity against chloroquine (CQ)-sensitive (Hb3) and CQ-resistant (Dd2) Plasmodium falciparum lines. Crude extracts and pure compounds from Vernonia guineensis showed antiplasmodial activity against both Hb3 and Dd2. The IC50 values of extracts ranged from 1.64 to 27.2 µg/ml for Hb3 and 1.82-30.0 µg/ml for Dd2; those for compounds 1, 2 and 3 ranged from 0.47 to 1.62 µg/ml (1364-1774 nM) for Hb3 and 0.57-1.50 µg/ml (1644-2332nM) for Dd2. None of the crude extracts or pure compounds was observed to exert toxic effects on the erythrocytes used to cultivate the Plasmodium falciparum lines. In Cameroonian folk medicine, Vernonia guineensis may be used to treat malaria in part due to the antiplasmodial activity of sesquiterpene lactones (1, 2), a sucrose ester (3) and perhaps other compounds present in crude plant extracts. Exploring the safety and antiplasmodial efficacy of these compounds in vivo requires further study. Copyright © 2013 Elsevier Ireland Ltd. All rights reserved.

  1. Glandular trichomes and essential oil characteristics of in vitro propagated Micromeria pulegium (Rochel) Benth. (Lamiaceae).

    Science.gov (United States)

    Stojičić, Dragana; Tošić, Svetlana; Slavkovska, Violeta; Zlatković, Bojan; Budimir, Snežana; Janošević, Dušica; Uzelac, Branka

    2016-08-01

    In vitro conditions and benzyladenine influenced both content and composition of micropropagated Micromeria pulegium essential oils, with pulegone and menthone being the main essential oil components. The content and chemical composition of Micromeria pulegium (Rochel) Benth. essential oils were studied in native plant material at vegetative stage and in micropropagated plants, obtained from nodal segments cultured on solid MS medium supplemented with N(6)-benzyladenine (BA) or kinetin at different concentrations, alone or in combination with indole-3-acetic acid. Shoot proliferation was achieved in all treatments, but the highest biomass production was obtained after treatment with 10 μM BA. Phytochemical analysis identified up to 21 compounds in the essential oils of wild-growing and in vitro cultivated plants, both showing very high percentages of total monoterpenoids dominated by oxygenated monoterpenes of the menthane type. Pulegone and menthone were the main essential oil components detected in both wild-growing plants (60.07 and 26.85 %, respectively) and micropropagated plants grown on either plant growth regulator-free medium (44.57 and 29.14 %, respectively) or BA-supplemented medium (50.77 and 14.45 %, respectively). The percentage of total sesquiterpenoids increased in vitro, particularly owing to sesquiterpene hydrocarbons that were not found in wild-growing plants. Differences in both content and the composition of the essential oils obtained from different samples indicated that in vitro culture conditions and plant growth regulators significantly influence the essential oils properties. In addition, the morphology and structure of M. pulegium glandular trichomes in relation to the secretory process were characterized for the first time using SEM and light microscopy, and their secretion was histochemically analyzed.

  2. New anti-trypanosomal active tetracyclic iridoid isolated from Morinda lucida Benth.

    Science.gov (United States)

    Suzuki, Mitsuko; Tung, Nguyen Huu; Kwofie, Kofi D; Adegle, Richard; Amoa-Bosompem, Michael; Sakyiamah, Maxwell; Ayertey, Frederick; Owusu, Kofi Baffour-Awuah; Tuffour, Isaac; Atchoglo, Philip; Frempong, Kwadwo Kyereme; Anyan, William K; Uto, Takuhiro; Morinaga, Osamu; Yamashita, Taizo; Aboagye, Frederic; Appiah, Alfred Ampomah; Appiah-Opong, Regina; Nyarko, Alexander K; Yamaoka, Shoji; Yamaguchi, Yasuchika; Edoh, Dominic; Koram, Kwadwo; Ohta, Nobuo; Boakye, Daniel A; Ayi, Irene; Shoyama, Yukihiro

    2015-08-01

    Human African trypanosomiasis (HAT), commonly known as sleeping sickness has remained a serious health problem in many African countries with thousands of new infected cases annually. Chemotherapy, which is the main form of control against HAT has been characterized lately by the viewpoints of toxicity and drug resistance issues. Recently, there have been a lot of emphases on the use of medicinal plants world-wide. Morinda lucida Benth. is one of the most popular medicinal plants widely distributed in Africa and several groups have reported on its anti-protozoa activities. In this study, we have isolated one novel tetracyclic iridoid, named as molucidin, from the CHCl3 fraction of the M. lucida leaves by bioassay-guided fractionation and purification. Molucidin was structurally elucidated by (1)H and (13)C NMR including HMQC, HMBC, H-H COSY and NOESY resulting in tetracyclic iridoid skeleton, and its absolute configuration was determined. We have further demonstrated that molucidin presented a strong anti-trypanosomal activity, indicating an IC50 value of 1.27 μM. The cytotoxicity study using human normal and cancer cell lines indicated that molucidin exhibited selectivity index (SI) against two normal fibroblasts greater than 4.73. Furthermore, structure-activity relationship (SAR) study was undertaken with molucidin and oregonin, which is identical to anti-trypanosomal active components of Alnus japonica. Overlapping analysis of the lowest energy conformation of molucidin with oregonin suggested a certain similarities of aromatic rings of both oregonin and molucidin. These results contribute to the future drug design studies for HAT. Copyright © 2015 Elsevier Ltd. All rights reserved.

  3. Proliferative effect of the aqueous extract of Hyptis pectinata on liver regeneration after partial hepatectomy in rats Efeito do extrato aquoso da Hyptis pectinata na regeneração hepática após hepatectomia parcial em ratos

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    Gustavo Barreto Melo

    2006-01-01

    Full Text Available PURPOSE: This study was carried out to assess the effects of the aqueous extract of Hyptis pectinata leaves on liver regeneration and on serum enzymes (AST, ALT and gamma-GT after 67% partial hepatectomy in rats. METHODS: AST, ALT and gamma-GT, were determined by conventional procedures using a spectrophotometer (Model E2250-CELM. Liver regeneration was evaluated by immunohistochemical staining for proliferating cell nuclear antigen (PCNA. RESULTS:Oral pretreatment during 4 days at 100 mg/kg increased liver regeneration index. At 200 mg/kg, AST level was statistically decreased in comparison to the group submited to distilled water administration. The other enzymes assessed disclosed no difference when all groups were compared. CONCLUSION: The present study shows that the aqueous extract of Hyptis pectinata leaves contains some biological active principles that stimulate liver regeneration at 100 mg/kg and cause slight hepatic protection at 200 mg/kg.OBJETIVO: Este estudo foi realizado com o objetivo de verificar o efeito do extrato aquoso da Hyptis pectinata na regeneração hepática bem como nos níveis das enzimas séricas (AST, ALT e gama-GT após hepatectomia parcial de 67% em ratos. MÉTODOS: AST, ALT e gama-GT, foram determinadas pelo método cinético utilizando um espectrofotômetro (Modelo E2250-CELM. A regeneração hepática foi avaliada por imunohistoquímica (PCNA. RESULTADOS: O pré-tratamento oral de 100 mg/kg foi realizado durante 4 dias e causou aumento na regeneração hepática O pré-tratamento oral com 200 mg/kg diminuiu significativamente os níveis de AST quando comparado com o grupo submetido ao pré-tratamento com água destilada. As demais enzimas avaliadas não apresentaram diferenças quando comparadas entre os grupos estudados. CONCLUSÃO: O presente estudo mostra que o extrato aquoso da Hyptis pectinata, numa concentração de 100 mg/kg possui alguma atividade biológica estimulando a regeneração hepática e

  4. Superação da dormência em sementes de faveira (Parkia platycephala Benth Dormancy breaking of faveira (Parkia platycephala Benth. seeds

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    Irinaldo Lima do Nascimento

    2009-02-01

    Full Text Available A faveira é planta semidecídua, heliófita, que ocorre em formações secundárias e áreas abertas de terreno elevado do agreste nordestino e campinas amazônicas. A madeira dessa espécie é empregada para caixotaria, compensados, brinquedos, lenha e carvão, as vagens maduras constituem-se em excelente forragem para todos os ruminantes e a árvore é recomendada para arborização paisagística. O primeiro problema encontrado foi a baixa germinação das sementes devido à impermeabilidade do tegumento à água. Dessa forma, este trabalho teve o objetivo de determinar a metodologia mais eficiente para superação da dormência de sementes de Parkia platycephala, as quais foram submetidas a 12 tratamentos: testemunha - sementes intactas (T1, escarificação mecânica com lixa d'água n. 80 (T2, imersão em ácido muriático concentrado (98% por 30 min e 1 h (T3 e T4, respectivamente, escarificação mecânica com brita por 5, 10 e 15 min (T5, T6 e T7, respectivamente e imersão em ácido sulfúrico concentrado (98% por 5, 15, 30, 45 e 60 min (T8, T9, T10, T11 e T12, respectivamente. Os efeitos dos tratamentos foram avaliados através da porcentagem, primeira contagem e índice de velocidade de emergência de plântulas, além de comprimento e massa seca da raiz e parte aérea. Utilizou-se o delineamento inteiramente casualizado com quatro repetições, e as médias foram comparadas pelo teste de Tukey a 5% de probabilidade. Houve diferença significativa entre os tratamentos em todas as variáveis. A causa mais evidente da dormência foi a impermeabilidade do tegumento, cujos tratamentos mais eficientes para superar a dormência das sementes foram a escarificação mecânica do tegumento com lixa e a imersão em ácido sulfúrico (15 a 45 min.The faveira (Parkia platycephala Benth. is a perennial, heliophyte, secondary plant that occurs in (non-flooded areas of the Amazon area. The wood of this species is used to make boxes, supports and

  5. Atividade inseticida do óleo essencial de Hyptis marrubioides no controle de Zabrotes subfasciatus (Coleoptera, Chrysomelidae, Bruchinae

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    Marcelo Barboza de Mello

    2014-02-01

    Full Text Available Sabe-se que uma grande porcentagem da produção e do armazenamento dos grãos de feijão é perdida pelo consumo de insetos. Este trabalho teve como objetivo avaliar a atividade inseticida do óleo essencial de Hyptis marrubioides no controle de Zabrotes subfasciatus. A extração do óleo essencial foi realizada pelo método de hidrodestilação em aparelho de Clevenger, por uma hora e meia e o ensaio biológico foi conduzido no Laboratório de Microscopia/IFSULDEMINAS, Campus Muzambinho. O delineamento experimental utilizado foi inteiramente casualizado, constituído de cinco concentrações de óleo essencial (0,0; 6,25%; 12,5%; 25% e 50%, com quatro repetições contendo um casal de Z. subfasciatus por repetição. Realizou-se o teste de mortalidade e inibição de oviposição. Utilizou-se três grãos de feijão tratados com 5 microlitros (μL de solução e três não tratados, caracterizando assim um teste com chance de escolha. Como controle, utilizou-se 100 μL de álcool 70%. Após a aplicação dos tratamentos, observou-se a reação dos indivíduos de Z. subfasciatus, evidenciando que os Tempos Letais (TL diminuem conforme há um aumento e contato direto dos insetos com as concentrações do óleo essencial de H. marrubioides. A aplicação do óleo essencial foi bastante efetiva, pois ocorreu mortalidade total dos insetos adultos e nenhuma oviposição. O intervalo de tempo de 28 minutos foi suficiente para provocar mortalidade de todos os indivíduos. Foi possível observar que o óleo essencial de H. marrubioides possui efeito inseticida sobre Z. subfasciatus, podendo auxiliar no manejo integrado de Z. subfasciatus em feijão armazenado.

  6. Escarificación artificial y natural de la semilla de Lupinus bilineatus Benth

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    J. M. Martínez

    2008-01-01

    Full Text Available Las especies de Lupinus colonizan áreas incendiadas y fijan N. En otros países las especies de este género se emplean en plantaciones forestales y sistemas agroforestales. Sus semillas presentan latencia física. En el presente estudio se realizó el análisis de semillas y se evaluó la germinación de Lupinus bilineatus Benth. considerando los siguientes factores y niveles: escarificación (natural: fuego, humo; artificial: inmersión en ácido sulfúrico durante 10, 20, 30 y 40 min y control, régimen de temperatura día/noche en cámaras de ambiente controlado (15/10, 20/15, 25/20, 30/25 °C y luz (con y sin. El diseño experimental fue en bloques al azar, con los bloques anidados dentro del factor temperatura. Se realizó un análisis de varianza mixto (con el procedimiento mixto de SAS y la prueba de Tukey. La pureza fue 97.1 %, el peso 66,666.7 semillas·kg-1, el contenido de humedad (base anhidra 7.1 % y la viabilidad 98 % (prueba de tetrazolio. Para la germinación, resultó significativa (P=0.0101 la interacción entre tratamiento escarificatorio, temperatura y luz. La máxima germinación (82.5 % se registró para semilla escarificada químicamente durante 30 o 40 min, a 25/20 °C y con luz. A mayor tiempo de escarificación química, mayor porcentaje de germinación. A 20/15 °C y con luz, la germinación posterior a la escarificación con fuego (38.5 % superó al control (18.5 %. Generalmente el control mostró valores de germinación menores a 30 % en los diferentes tratamientos, denotando latencia física con diferentes intensidades en el lote. Destaca la eficiencia de la escarificación química para cesar la latencia.

  7. Estudo das folhas e caule de Hyptidendron canum(Pohl ex Benth. Harley, Lamiaceae

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    Tatiana S. Fiuza

    Full Text Available Hyptidendron canum (Pohl ex Benth. Harley, Lamiaceae, é utilizada popularmente como antimalárica, antiinflamatória, antiulcerativa, anti-hepatotóxica e anticancerígena. O objetivo deste trabalho foi realizar o estudo morfo-anatômico das folhas e caules e identificar as principais classes de metabólitos secundários presentes nas folhas de H. canum, dados ainda não descritos na literatura. As folhas e caules jovens coletados em Goiânia (GO foram seccionados à mão livre e preparados para análise microscópica. Foram realizadas reações de identificação de metabólitos secundários do material dessecado e pulverizado. Preparou-se o extrato etanólico bruto, que posteriormente foi fracionado por partição líquido-líquido com hexano, clorofórmio e acetato de etila. As frações foram submetidas à análise cromatográfica em camada delgada (CCD. As lâminas foliares apresentam epiderme adaxial constituída por células poligonais com parede reta. Na epiderme abaxial observam-se células com parede reta a ondulada e estômatos diacíticos e anisocíticos. Tricomas tectores e glandulares estão presente em ambas as faces da lâmina foliar. O pecíolo apresenta aspecto canaletado, epiderme adaxial e abaxial unisseriada. O caule, em secção transversal possui contorno em geral quadrangular, com presença de tricomas tectores e glandulares. As reações e a CCD das folhas evidenciaram a presença de flavonóides, saponinas, terpenos e lignanas. Este trabalho contribuiu para um maior conhecimento da morfo-anatomia e das classes químicas presentes em H. canum.

  8. Antitrypanosomal activity of Verbascum sinaiticum Benth. (Scrophulariaceae) against Trypanosoma congolense isolates.

    Science.gov (United States)

    Mergia, Ermias; Shibeshi, Workineh; Terefe, Getachew; Teklehaymanot, Tilahun

    2016-09-15

    African Trypanosomiasis is a neglected tropical disease with a large impact on the livelihood of the rural poor in Sub-Saharan Africa. The available drugs for managing this disease are old, expensive and are facing the problem of drug resistance. Thus, the aim of this study was to evaluate in vivo antitrypanosomal efficacy of aqueous and absolute methanol leaf extracts of Verbascum sinaiticum Benth. against Trypanosoma congolense field isolate. Verbascum sinaiticum (Local name 'qetetina') is a biennial plant, and 60-150 cm tall. It is traditionally used to treat wound, stomachache, viral infection, cancer, sunstroke, fever, abdominal colic, diarrhea, hemorrhage, anthrax, and hepatitis. The efficacy of aqueous and absolute methanol leaf extracts of V. sinaiticum was evaluated in a randomized experiment with Swiss albino mice infected with T. congolense field isolate. The extracts were administered at doses of 100, 200 and 400 mg/kg by intraperitoneal injection for seven days at 12 Days Post-Infection (DPI) when the peak parasitaemia level was approximately 10(8) trypanosomes/ml. Parasitaemia, Packed Cell Volume (PCV), mean survival time and change in body weight were used as indices for monitoring the efficacy of the extracts. Diminazene (28 mg/kg) was used as a positive control while 2 % Tween was used as the negative control. Phytochemicals screening were conducted following standard methods. The extracts showed no toxicity effect in Swiss albino mice and had LD50 above 2000 mg/kg. The phytochemicals screened in V. sinaiticum were alkaloids, flavonoids, glycoside, saponins, steroids, phenolic compounds, and tannins. The mice treated with absolute methanol leaf extract of V. sinaiticum at 400 mg/kg dose had significantly lower mean parasitaemia (7.20 ± 0.16) (p < 0.001) as compared to the negative control group (8.82 ± 0.12) on day 14 of treatment. Animals treated with the same dose had significant (p < 0.001) higher PCV value and body

  9. Histologic evaluation of rats' liver after Croton cajucara Benth (sacaca administration Estudo histológico do fígado de ratos após administração de Croton Cajucara Benth (sacaca

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    Jair Francisco de Santana Graim

    2008-04-01

    Full Text Available PURPOSE: To evaluate the hepatic effect of Croton cajucara Benth (sacaca herbal extract in rats. METHODS: 60 Wistar rats (Rattus novergicus albinus were used, weighing between 250 and 350 g. The animals were distributed randomically in 2 groups: GS - animals which received daily 0,1 ml/ 100 g of sacaca herbal extract through gavage, and GA -animals which received daily 0,1 ml/100g of distilled water through gavage. These were distributed in 3 subgroups with 10 animals, according to theirs euthanasia dates, which were 14th, 28th and 56th day of treatment. RESULTS: Architectural alterations were not observed, however when it was analyzed the presence or absence of necrosis, it was observed in 50% of GS28 subgroup and 90% of subgroup. In 50% of the animals from GS28 subgroup and 90% of GS56 subgroup was observed vast degeneration areas and zonal necrosis, regarding center-lobular veins alterations, there were no alterations in any of the groups CONCLUSION: The Croton cajucara Benth (sacaca herbal extract in this experiment caused degeneration and hepatic necrosis, suggesting dose-dependent action.OBJETIVO: Avaliar histologicamente os efeitos do infuso, por gavagem, do Extrato Bruto Aquoso Seco (EBAS do Croton cajucara Benth no fígado de ratos. MÉTODOS: Foram utilizados 60 ratos (Rattus novergicus albinus Wistar, pesando entre 250 e 350g. Os animais foram distribuídos de maneira aleatória em 2 grupos: GS - Grupo de animais que receberam 0,1ml/100g do infuso de sacaca, pela via oral e GA - Grupo de animais que recebeu 0,1ml/100g de água destilada, pela via oral. Estes foram distribuídos em 3 subgrupos de acordo com o dia da eutanásia, sendo esta realizada no 14º, 28º e 56º dia, cada um com 10 animais. RESULTADOS: Os resultados obtidos demonstraram que, microscopicamente, não houve alterações arquiteturais, porém quando se analisou a necrose hepatocitária, esta esteve presente em 50% do subgrupo GS28 e 90% do subgrupo GS56; as altera

  10. Variação química do óleo essencial de Hyptis suaveolens (L. Poit., sob condições de cultivo

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    Felipe Terra Martins

    2006-12-01

    Full Text Available This study was performed to establish the correlation between the growth conditions and essential oil composition of Hyptis suaveolens from Alfenas (MG, Brazil. The plants were grown in a greenhouse, four treatments were used and they were harvested at two different periods of time (60 and 135-day-old plants. The essential oil composition was determined by GC-MS analysis. The terpenes spathulenol, globulol, dehydroabietol, a-cadinol and beta-phellandrene were the major constituents found in the essential oil. Oxygenated sesquiterpenes represented the main group of constituents in most of the treatments. The major changes in the essential oil composition were found in 135-day-old plants grown under NPK deficiency. We also identified three groups of volatile components that have not been previously described in H suaveolens.

  11. A novel 8.7 kDa protease inhibitor from chan seeds (Hyptis suaveolens L.) inhibits proteases from the larger grain borer Prostephanus truncatus (Coleoptera: Bostrichidae).

    Science.gov (United States)

    Aguirre, Cesar; Valdés-Rodríguez, Silvia; Mendoza-Hernández, Guillermo; Rojo-Domínguez, Arturo; Blanco-Labra, Alejandro

    2004-05-01

    A novel trypsin inhibitor purified from chan seeds (Hyptis suaveolens, Lamiaceae) was purified and characterized. Its apparent molecular mass was 8700 Da with an isoelectric point of 3.4. Its N-terminal sequence showed a high content of acidic amino acids (seven out of 18 residues). Its inhibitory activity was potent toward all trypsin-like proteases extracted from the gut of the insect Prostephanus truncatus (Coleoptera: Bostrichidae), a very important pest of maize. This activity was highly specific, because among proteases from seven different insects, only those from P. truncatus and Manduca sexta (Lepidoptera: Sphingidae) were inhibited. This inhibitor has potential to enhance the defense mechanism of maize against the attack of P. truncatus.

  12. Pharmacological activities and medicinal properties of endemic Moroccan medicinal plant Origanum compactum (Benth and their main compounds

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    Abdelhakim Bouyahya

    2017-10-01

    Full Text Available Oregano [Origanum compactum Benth. (O. compactum, Lamiaceae] is an endemic Moroccan medicinal herb. It is used traditionally to fight against several disorders such as diarrhea, urolithiasis, hypertension, diabetes, and inflammation. A large number of components have been identified and isolated from the essential oil of this plant. Carvacrol, thymol, p-cymene and γ-Terpinene are among the more compounds presented in O. compactum essential oil and considered to be the main biologically active components. Numerous experimental studies showed that O. compactum organic extracts, essentials oil and its main compounds possess a broader spectrum of pharmacological and therapeutic activities such as antibacterial, antifungal, antioxidant, and anticancer activity. The present review attempts to give an overview of pharmacological studies of O. compactum and its major compounds.

  13. GERMINAÇÃO DE SEMENTES DE SABIÁ (MIMOSA CAESALPINIAEFOLIA BENTH. E ALGAROBA (PROSOPIS JULIFLORA (SW DC

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    Salvador Barros Torres

    1994-12-01

    Full Text Available Estudou-se o efeito da temperatura e do substrato sobre a germinação de sementes de sabiá (Mimosa caesalpiniaefolia Benth. e algaroba (Prosopis juliflora (SW DC em condições de laboratório. Foram testadas as temperaturas de 25°C e 30°C constantes e 20-30°C alternadas em substratos de papel toalha, papel mata-borrão e areia. O melhor resultado de germinação para as sementes de sabiá foi obtido com a temperatura de 20-30°C em substrato de papel mata-borrão e a mesma temperatura em substrato de areia, para sementes de algaroba.

  14. Caracterização farmacognóstica da espécie Erythrina falcata Benth., Fabaceae

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    Emanuel Eustáquio Almeida

    Full Text Available Erythrina falcata Benth., Fabaceae, conhecida popularmente no Brasil pelos nomes de mulungu, sapatinho-de-judeu, corticeira-da-serra, muchoco, é usada pela população como planta medicinal indicada como sedativo, ansiolítico ou para doenças do aparelho respiratório. Seu uso indiscriminado, no entanto, pode trazer consequências graves à saúde, devido à forte presença de alcaloides, particularmente nas sementes e na casca. O presente trabalho tem como objetivo estudos fitoquímicos para identificação dos principais grupos de substâncias existentes na espécie, além de estudos morfológicos e histológicos para diferenciação entre outras espécies do gênero.

  15. Cathepsin B cysteine protease gene is upregulated during leaf senescence and exhibits differential expression behavior in response to phytohormones in Picrorhiza kurrooa Royle ex Benth.

    OpenAIRE

    Jai Parkash; Sanjeeta Kashyap; Shruti Kirti; Anil Kumar Singh; Som Dutt

    2015-01-01

    Medicinal importance of Picrorhiza (Picrorhiza kurrooa Royle ex Benth — an herb of western Himalayan region) and its endangered status in Red Data Book presses an urgent need for intensive R&D interventions towards ensuring its availability for the medicinal use, its sustainability and improvement. The present study was conducted on cathepsin B cysteine protease in Picrorhiza. Cathepsin B cysteine protease has been reported to function in diverse processes such as senescence, abscission, prog...

  16. Actividad citotóxica, anti-inflamatoria y anti-ulcerogénica de plantas silvestres e in vitro de castilleja tenuiflora benth.

    OpenAIRE

    Sanchez Ocampo, Paul Mauricio

    2016-01-01

    Castilleja tenuiflora Benth. (Orobanchaceae) es una planta usada en la medicina tradicional mexicana para tratar tumores, esterilidad, desordenes gastrointestinales y cirrosis. Se han identificado iridoides, feniletanoides y flavonoides en plantas silvestres y en cultivos in vitro de C. tenuiflora, los cuales están asociados a diversas actividades biológicas como antioxidante, antitumoral, citotóxica, anti-inflamatoria e inmunoestimulante. Por estos antecedentes la planta se pr...

  17. Isolasi Dan Analisis Komponen Kimia Minyak Atsiri Dari Daun Jinten (Coleus Aromatikus Benth) Dengan GC – MS Dan Uji Anti Bakteri

    OpenAIRE

    Sinulingga, Bagus

    2011-01-01

    The research was conducted whit the scale of laboratore. The object ofthis research was fresh leave jinten. The treatment done was to isolatethe essential oil of leave jinten (Coleus Aromaticus Benth) though water distillation . To analyzed chemical components was done with mass Spectrometry – gas Cromatography. The reuslt of gas cromatography showed 15 apexes meaning that the essensial oil of the leave jinten. Based on analysis with spectrometry mass inducted that chemical compound in...

  18. Evaluación de un sistema para la micorrización in vitro en plantas de mora de castilla (Rubus glaucus, Benth

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    Víctor Manuel Núñez-Zarante

    2012-08-01

    Full Text Available Evaluation of an in vitro mycorrhization system of blackberry plants (Rubus glaucus, Benth. Objective. Obtain an in vitromycorrhization system in autotrophic culture systems of blackberry plants (Rubus glaucus, Benth. Materials and methods. We usedspores and root fragments with vesicles of Arbuscular Mycorrhizal Fungus (AMF Glomus sp (GEV02. We established an autotrophicculture system of blackberry plantlets comparing two methods of direct inoculation of the AMF. We measured the number of sporesproduced, the length of the extraradical mycelium as well as the percentage of colonization of the AMF. Additionally, we measuredthe shoot and root length, and the fresh and dry weight of the leaf and root parts to determine the plant development. Results. Theautotrophic culture system was successful for blackberry plants (Rubus glaucus, Benth; an optimal shoot and root growth was observed.Additionally, we obtained a system that allowed the development of Glomus sp. in in vitro conditions, with the formation of structurestypical of the symbiosis as well as a good intraradical colonization, with the production of arbuscules and vesicles, development ofextraradical mycelium with branched hyphae, and formation of new spores. Conclusion. For the first time, micropropagated blackberryplants associated successfully with an AMF under in vitro conditions, enabling the development of the symbiotic system AMF Glomussp. associated to roots of micropropagated blackberry plantlets.

  19. Bush mint (Hyptis suaveolens) and spreading hogweed (Boerhavia diffusa) medicinal plant extracts differentially affect activities of CYP1A2, CYP2D6 and CYP3A4 enzymes.

    Science.gov (United States)

    Ekow Thomford, Nicholas; Dzobo, Kevin; Adu, Faustina; Chirikure, Shadreck; Wonkam, Ambroise; Dandara, Collet

    2018-01-30

    Hyptis suaveolens (L) Poit and Boerhavia diffusa Linn are medicinal herbal plants commonly found in the tropics and sub-tropics. They are used to treat various conditions among them boils, dyslipidaemia, eczema, malaria, jaundice and gonorrhoea. Thus, the herbal medicinal extracts are now found as part of some commercial herbal formulations. There has not been adequate characterization of these medicinal herbs on their effects on drug metabolising enzymes. To investigate the effects of extracts of Hyptis suaveolens (HS) and Boerhavia diffusa (BD) on activity of drug metabolising enzymes, CYP1A2, CYP2D6 and CYP3A4, as well predict their potential for herb-drug interaction. A secondary aim was to identify constituent compounds such as polyphenolics, in the crude extract preparations of Hyptis suaveolens and Boerhavia diffusa and measure them for activity. CYP450 inhibition assays using recombinant CYP450 (rCYP) and fluorescence screening employing individual isozymes (CYP1A2, CYP2D6 and CYP3A4) were used to determine reversible- and time-dependent inhibition (TDI) profiles of extracts of Hyptis suaveolens and Boerhavia diffusa. Inhibition kinetic parameters, Ki and Kinact were also estimated. UPLC-MS employing a Synapt G2 (ESI negative) coupled to a PDA detector was used to identify polyphenolic compounds in crude extracts of Hyptis suaveolens and Boerhavia diffusa. The inhibitory potency of Hyptis suaveolens and Boerhavia diffusa extracts varied among the different enzymes, with CYP1A2 (3.68 ± 0.10µg/mL) being the least inhibited by HS compared to CYP2D6 (1.39 ± 0.01µg/mL) and CYP3A4 (2.36 ± 0.57µg/mL). BD was most potent on CYP3A4 (7.36 ± 0.94µg/mL) compared to both CYP2D6 (17.79 ± 1.02µg/mL) and CYP1A2 (9.48 ± 0.78µg/mL). Extracts of Hyptis suaveolens and Boerhavia diffusa exhibited TDIs on all CYPs. The most prominent phenolic candidates identified in both medicinal herbs using UPLC-MS analysis included caffeic acid, rutin, quercetin, citric acid

  20. Influência do tamanho e da procedência de sementes Mimosa caesalpiniifolia Benth. Sobre a germinação e vigor Influence of size and provenance on the germination and vigor of Mimosa caesalpiniifolia Benth. Seeds

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    Edna Ursulino Alves

    2005-12-01

    Full Text Available Mimosa caesalpiniifolia Benth. é uma planta nativa da Região Nordeste que vem sendo progressivamente cultivada do Maranhão ao Rio de Janeiro. A planta apresenta grande potencial para arborização, cerca viva e produção de madeira. O presente trabalho teve como objetivo avaliar a influência da procedência e tamanho das sementes sobre a germinação e vigor. Para tanto, realizou-se um experimento no Laboratório de Análise de Sementes do Centro de Ciências Agrárias da Universidade Federal da Paraíba (CCA-UFPB, em Areia, PB, em delineamento experimental inteiramente casualizado com os tratamentos distribuídos em esquema fatorial 3x3, com os fatores procedência (Areia, Usina e Arara e classes de tamanho (sementes pequenas, médias e grandes, em quatro repetições de 25 sementes. Foram analisadas as seguintes características: peso de 100 sementes, dimensões das sementes (comprimento, largura e espessura, porcentagem, primeira contagem e velocidade de germinação, comprimento e massa seca da raiz primária e hipocótilo e massa seca dos cotilédones. Constatou-se que a germinação não foi influenciada pelo tamanho das sementes, no entanto, ela foi significativamente influenciada pela procedência. Os testes de primeira contagem e de velocidade de germinação não se mostraram adequados para avaliação do vigor das sementes, sendo este mais bem avaliado pela massa seca dos cotilédones e hipocótilo. O vigor das sementes, apresentou relação direta com o seu tamanho, justificando-se a adoção de classes de tamanho para a formação de mudas.Mimosa caesalpiniifolia Benth. is a native species from the Northeast region, Brazil, which has been increasingly cultivated from the States of Maranhão to Rio de Janeiro. It presents great potential for urban forestry, hedging and wood production. The present research had as objective to evaluate the influence of the provenance and seed size on the germination and vigor of Mimosa

  1. Teor e composição química do óleo essencial de Hyptis marrubioides Epl., Lamiaceae em função da sazonalidade = Content and chemical composition of Hyptis marrubioides essential oil in function of seasons

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    Suzan Kelly Vilela Bertolucci

    2010-07-01

    Full Text Available Os oleos essenciais sao principios odoriferos armazenados em celulas especiais da planta. O oleo essencial no genero Hyptis e usado como anestesico, antiespasmodico, antiinflamatorio e pode induzir aborto em doses elevadas. O objetivo deste trabalho foi avaliar a influencia da sazonalidade no teor e composicao do oleo essencial de Hyptis marrubioides. O delineamento experimental foi inteiramente casualizado, com quatro tratamentos (inverno, primavera, verao e outono e seis repeticoes, totalizando 60 plantas. O oleo essencial foi extraido por hidrodestilacao, em aparelho de Clevenger. A analise qualitativa do oleo essencial foi por cromatografia em fase gasosa (CG/EM. Na estacao do verao, as folhas das plantas de H. marrubiodes apresentaram os maiores teores de oleo essencial. Nas demais estacoes, os teores de oleo essencial obtidos foram semelhantes entre si. Os componentes majoritarios no oleo essencial foram os monoterpenoides α-tujona e β-tujona. As concentracoes relativas dos picos correspondentes a β-tujona nao apresentaram diferencas consideraveis nas quatro estacoes. Os sesquiterpenoides oxigenados (cedrol e cariofilenol e nao-oxigenados (α-copaeno, β-cariofileno, germacreno D e cadaleno foram encontrados em menores quantidades no oleo essencial, entretanto observam-se diferencas quantitativas ao longo das estacoes.Essential oils are odorous principles stored in special plant cells. The essential oil in the Hyptis genus is traditionally used as an anesthetic, antispasmodic, antiinflammatory, and can induce abortion in high doses. The purpose of this work was to evaluate the influence of seasons in essential oil content and chemical composition of species H. marrubioides. A completely randomized complete design was used with four treatments (winter, spring, summer and autumn and six replications, totaling 60 plants. The essential oil was obtained by hydrodistillation with a Clevenger apparatus. The qualitative analysis of the

  2. Variações no teor e na composição volátil de Hyptis marrubioides EPL: cultivada no campo e em casa de vegetação Variation in the content and volatile composition of Hyptis marrubioides EPL: cultivated in field and greenhouse

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    Priscila Pereira Botrel

    2010-01-01

    Full Text Available This work describes the chemical composition of the volatile oil of Hyptis marrubioides cultivated in field and greenhouse. The experimental design was completely randomized, with ten replications for each type of cultivation. The volatile oil was extracted by hydrodistillation and analyzed by GC-MS. The highest content of volatile oil was found for plants grown in field. The highest percentage of the compounds present in oils was observed in samples grown in the field, such as germacra-4(15,5,10(14-trien-1-α-ol (16.34%, β-caryophyllene (10.42%, γ-muurolene (12.83% and trans-thujone (9.98%. However, some compounds were found only in plants grown in a greenhouse, such as cis-muurol-5-en-4α-ol (10.84%, α-cadinol (3.06% and eudesma-4(15,7-dien-1β-ol (6.82%.

  3. Chemical and Antimicrobial Analyses of Sideritis romana L. subsp. purpurea (Tal. ex Benth.) Heywood, an Endemic of the Western Balkan.

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    Tadić, Vanja; Oliva, Alessandra; Božović, Mijat; Cipolla, Alessia; De Angelis, Massimiliano; Vullo, Vincenzo; Garzoli, Stefania; Ragno, Rino

    2017-08-23

    A comprehensive study on essential oil and different solvent extracts of Sideritis romana L. subsp. purpurea (Tal. ex Benth.) Heywood (Lamiaceae) from Montenegro is reported. The gas chromatography-mass spectrometry analysis of the essential oil revealed a total of 43 components with bicyclogermacrene (23.8%), germacrene D (8%), (E)-caryophyllene (7.9%) and spathulenol (5.5%) as the major ones. Sesquiterpenoid group was found to be the most dominant one (64.8%), with 19.9% of the oxygenated forms. In the crude methanol extract of the investigated plant, obtained by Sohhlet exraction, the total phenol content was 14.7 ± 0.4 mg of GA/g, the total flavonoids were 0.29 ± 0.03% expressed as hyperoside percentage, whereas the total tannins content was 0.22 ± 0.04% expressed as pyrogallol percentage. For the antimicrobial activity determination, the following microorganisms have been used: methicillin-susceptible Staphylococcus aureus (MSSA (American Type Culture Collection (ATCC) 29213)) and methicillin-resistant S. aureus (MRSA (clinical strain)), Escherichia coli (ATCC 25922), carbapenem-susceptible Klebsiella pneumoniae (clinical strain), carbapenem-resistant K. pneumoniae (clinical strain) and Candida albicans (ATCC 14053). The essential oil showed high potency against MSSA and MRSA, both at high (~5 × 10⁵ CFU/mL) and low (~5 × 10³ CFU/mL) inoculum. With respect to MSSA, the minimal inhibitory concentration (MIC) value was 0.307 mg/mL, with bactericidal activity obtained at 0.615 mg/mL, while, in the case of MRSA, the MIC and minimal bactericidal concentration (MBC) values were 0.076 and 0.153 mg/mL, respectively. Regarding anti-Candida albicans activity, the MIC value was 2.46 mg/mL without reaching fungicidal activity. In addition to the observed essential oil efficacy, different solvent extracts were analyzed for their antimicrobial activity. Similarly to the essential oil, thehighest efficacy was observed against both MSSA and MRSA strains, at high and

  4. Oral treatment with essential oil of Hyptis spicigera Lam. (Lamiaceae) reduces acute pain and inflammation in mice: Potential interactions with transient receptor potential (TRP) ion channels.

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    Simões, Róli Rodrigues; Coelho, Igor Dos Santos; Junqueira, Stella Célio; Pigatto, Glauce Regina; Salvador, Marcos José; Santos, Adair Roberto Soares; de Faria, Felipe Meira

    2017-03-22

    The genus Hyptis comprehends almost 400 species widespread in tropical and temperate regions of America. The use of Hyptis spicigera Lam. (Lamiaceae) is reported in traditional medicine due to its gastroprotective, anti-inflammatory and analgesic properties. The rationale of this study was to investigate the potential use of the essential oil of H. spicigera (EOHs) as analgesic. The antinociceptive effect of EOHs was verified analyzing acute nocifensive behavior of mice induced by chemical noxious stimuli [i.e., formalin and transient receptor potential (TRP) channels agonists]. We also verified the effects of EOHs on locomotor activity and motor performance in mice. Finally, we investigate the involvement of central afferent C-fibers with EOHs analgesic effect. EOHs presented antinociceptive effect at 300 and 1000mg/kg on formalin-induced pain behavior model, presenting 50% and 72% of inhibition during the first phase (ED50 =292mg/kg), and 85% and 100% during de second phase (ED50 =205mg/kg), respectively. Temperature of the hind paw was reduced by EOHs treatment in a dose-dependent manner; oedema was diminished only by EOHs 1000mg/kg. EOHs does not impaired locomotor activity or motor performance. For mice injected with capsaicin, a TRPV1 activator, EOHs (1000mg/kg, ED50 =660mg/kg) showed decreased (63%) nociceptive behavior. When injected with cinnamaldehyde (TRPA1 activator), mice treated with EOHs showed 23%, 43% and 66% inhibition on nociceptive behavior (100, 300 and 1000mg/kg, respectively; ED50 402mg/kg). When mice were injected with menthol (TRPM8 activator), EOHs showed 29%, 59% and 98% inhibition of nociceptive behavior (100, 300 and 1000mg/kg, respectively; with ED50 =198mg/kg. Finally, when desensitized mice were injected with menthol, EOHs (300mg/kg) does not show antinociceptive effect. This study demonstrated the efficacy of EOHs on experimental models of nociception. We have found the involvement of TRP channels V1, A1 and M8 with EOHs activity

  5. The tritrophic system hyptis suaveolens (Lamiaceae - agromyzid leafminers (Diptera: Agromyzidae - parasitoids (Hymenoptera: effects of herbivore density, host plant patch size, and habitat complexity on parasitism rate

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    Jarbas Marcal de Queiroz

    2009-06-01

    Full Text Available In this study a tritrophic system was evaluated to measure the contributions of the insect host density and its host plant concentration in simple and diversified habitat on the rate of parasitism. The system was composed of the plant Hyptis suaveolens, two species of agromyzid leafminers, and three morphospecies of parasitoids. The parasitism rate, patch size, and habitat complexity were found to be interdependent. If only the habitat complexity or patch size was take into account the differences in parasitism rate are not significant, although habitat diversity seemed to contribute more than the host plant concentration to increase the parasitism rate. The leaves or plants with different number of mines were equally attacked by the parasitoids. The results were representative of what happened to the leafminers at different host plant densities under the homogeneous and heterogeneous habitat condition. This work could help to reinforce the idea of the importance of the plant diversity for enhancing the biological control of the pests by the parasitoids in the managed ecosystems.Foi avaliado um sistema tritrófico para medir os efeitos da densidade de insetos minadores e a concentração da planta hospedeira em habitats simples e diversificados sobre a taxa de parasitismo. O sistema era composto pela planta Hyptis suaveolens, duas espécies de agromizídeos minadores de folhas e três morfoespécies de parasitóides. A taxa de parasitismo, tamanho da mancha e a complexidade do habitat foram interdependentes. Se levarmos em consideração apenas a complexidade ou tamanho de mancha, as diferenças na taxa de parasitismo não foram significativas, embora a diversidade de habitat pareça ter contribuido mais do que a concentração da planta hospedeira para aumentar a taxa de parasitismo. Folhas ou plantas com diferentes números de minas foram igualmente parasitadas. Nossos resultados são representativos do que acontece com insetos minadores em

  6. Flavonoids and their derivatives with β-amyloid aggregation inhibitory activity from the leaves and twigs of Pithecellobium clypearia Benth.

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    Wang, Yu-Xi; Ren, Qiang; Yan, Zhi-Yang; Wang, Wei; Zhao, Lu; Bai, Ming; Wang, Xiao-Bo; Huang, Xiao-Xiao; Song, Shao-Jiang

    2017-11-01

    To explore potential compounds with marked effect on Alzheimer's disease (AD) in Pithecellobium clypearia Benth., nineteen compounds (1-19) were obtained, including two new flavonoid derivatives, named pithecellobiumol A (1) and pithecellobiumol B (2) and 17 flavonoids (3-19). Their structures were elucidated based on 1D and 2D-NMR spectra as well as HR-ESI-MS data. The absolute configurations of new compounds were assigned by comparing their experimental specific rotation or ECD curves with the calculated data. The inhibitory activity on Aβ aggregation was screened by ThT assay, and compounds 7 (70.7%), 9 (86.5%), 10 (88.4%), 15 (86.1%) and 16 (87.7%) showed outstanding inhibition rate at 20μM compared to the positive control, curcumin (65.64%). In addition, docking study was performed to initially examine possible molecular mechanisms. Considering the important role of oxidative stress in AD, all the isolated compounds were tested for their H2O2-induced damage in human neuronblastoma SH-SY5Y cells. Among them, compound 16 (91.0%) was the most potent candidate in the treatment of AD. Copyright © 2017 Elsevier Ltd. All rights reserved.

  7. Validated high-performance liquid chromatographic method for the standardisation of Ptychopetalum olacoides Benth., Olacaceae, commercial extracts

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    Renata Colombo

    2010-10-01

    Full Text Available Ptychopetalum olacoides Benth., Olacaceae, popularly known as marapuama or muirapuama or miriantã, is a species native to the Amazonian region of Brazil. Extracts of the bark of the plant have been used traditionally for its stimulating and aphrodisiac properties and currently commercialised by the herbal industry as constituents in a wide range of phytomedicines. Fractionation by open column chromatography followed by preparative HPLC-UV/PAD of the stem bark and of three commercial extracts of P. olacoides allowed the isolation of three components that were common to all extracts analysed, and these were identified by NMR to be vanillic acid, protocatechuic acid and theobromine. Vanillic acid, which has been proposed as a phytochemical marker for P. olacoides, was employed as an external standard in the development and validation of a rapid qualitative and quantitative HPLC assay for the analyte. The recoveries values of the developed method were 99.02% and the LOD and LOQ values were 0.033 and 0.11 mg.L-1, respectively. The described method may be applied to the standardisation of herbs, extracts or phytomedicines commercialised as marapuama.

  8. Evaluation of in vivo and in vitro anti-inflammatory activity of Ajuga bracteosa Wall ex Benth

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    Raghunath Singh

    2012-05-01

    Full Text Available Ethnopharmacological relevance: Ajuga bracteosa Wall Ex Benth. (Labiateae is described in Ayurveda for the treatment of rheumatism, gout, palsy and amenorrhea.Objective: Present study was aimed to investigate the in vivo and in vitro anti-inflammatory activity of Neelkanthi (whole plant and to support its traditional use.Methods: Methanolic extract of plant Ajuga bracteosa (ABE was investigated for its anti-inflammatory activity in carrageenan induced rat paw oedema, egg albumin induced inflammation in rats and the study was further supported with in vitro antiinflammatory study by using Human red blood cell membrane stabilization (HRBC method. Three doses of the extract (ABE- 250, 500 and 750 mg/kg, i.p. were used in the study and diclofenac sodium (5mg/kg, i.p. was used as standard. Results: ABE (500 and 750 mg/kg, i.p. significantly (P<0.05 reduced increased in paw volume induced by carrageenan and egg albumin. ABE also showed significant stabilization toward HRBC membrane. Conclusions: ABE at the dose of 500 and 750 mg/kg showed potent action on comparison with the standard drug diclofenac sodium.

  9. Cryopreservation of shoot tips of Picrorhiza kurroa Royle ex Benth, an indigenous endangered medicinal plant, through vitrification.

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    Sharma, Neelam; Sharma, Bindu

    2003-01-01

    The cryopreservation of shoot tips of Picrorhiza kurroa Royle ex Benth (IC 266698), an endangered medicinal plant of India was investigated. Shoot tips (about 1 mm in length) excised from four-week-old proliferating shoot cultures were precultured on MS medium supplemented with various osmotica before dehydrating with PVS2 solution at 0 degrees C. The dehydrated shoot tips were directly immersed in LN2. Following cryopreservation, and after rapid rewarming at 45 degrees C, shoot tips were quickly washed with 1.2 M sucrose solution and then plated on solidified shoot culture medium. Shoot tips were successfully cryopreserved by vitrification, when they were precultured on medium supplemented with 5% DMSO at 4 degrees C for two days before dehydrating in PVS2 for 10-20 minutes at 0 degrees C. Average survival in terms of normal shoot formation after 4 wks of plating was about 20% without callus formation. Cold hardening of shoot cultures for four weeks at 4 degrees C significantly improved the survival and shoot regeneration of cryopreserved shoot tips to 70% and 35%, respectively.

  10. Activités insecticides de Striga hermonthica (Del. Benth (Scrophulariaceae sur Callosobrichus maculatus (Fab. (Coleoptera : Bruchidae

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    Nacoulma OG.

    2006-01-01

    Full Text Available Insecticidal activities of Striga hermonthica (Del. Benth (Scrophulariacecae on Callobruchus maculatus (Fab. (Coleptera Bruchidae. This paper deals with insecticidal potentialities of Striga hermonthica (Del. (Scrophulariaceae in protection of cowpea Vigna unguculata (L. Walp against Callosobruchus maculatus (Fab. (Coleoptera: Bruchidae during storage. Crude acetone extract at 0,5% w/w (100 mg of extract for 20 g of grain exhibits 48% of ovicidal effect and then reduces by half emergence rate of adult beetles at the first generation. This extract shows a weak insecticide activity against adults of C. maculatus. Petroleum ether fraction (0,4% w/w of the crude extract reveals ovicidal (51% and larvicidal (72% effects which reduce the emergence rate of adults to only 9%. LD50 and LD90 are monitored during crude extract fractionation to follow ovicidal and larvicidal compounds and to evaluate their efficacy during the isolation procedure. One fraction, mainly composed of two triterpenoid compounds has been identified as responsible of the ovicidal activity of S. hermonthica while the origin of the larvicidal activity hasn’t been identified.

  11. Antibacterial Activity of Indian Borage (Plectranthus amboinicus Benth Leaf Extracts in Food Systems and Against Natural Microflora in Chicken Meat

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    Sandeep Kumar Gupta

    2016-01-01

    Full Text Available The ability of acetone and ethyl acetate extracts of the leaves of a traditional Indian medicinal plant, Indian borage (Plectranthus amboinicus Benth to prevent spoilage of artificially inoculated model food systems (cabbage and papaya and natural microflora of chicken meat was evaluated. These extracts were able to reduce the bacterial counts in all food systems; however, the effective concentration varied with the complexity of the system (in descending order: cabbage, papaya and chicken. A probable mode of action of extracts was investigated by analyzing the changes they cause in bacterial cell wall and leakage of nucleic acid from bacterial cells. Both acetone and ethyl acetate extracts at their respective minimum inhibitory concentrations resulted in leakage of cell constituents to an extent of 40 to 80 and 60 to 95 %, respectively, compared to the control, and finally leading to disintegration of cell walls. These findings indicate the potential use of ethyl acetate and acetone extracts of Indian borage leaves in food preservation.

  12. KANDUNGAN KIMIA BERBAGAI EKSTRAK DAUN MIANA (COLEUS BLUMEI BENTH DAN EFEK ANTHELMINTIKNYA TERHADAP CACING PITA PADA AYAM

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    Yusuf Ridwan

    2006-08-01

    Full Text Available Study on the chemical compound of painted nettle (Coleus blumei Benth leave extract and its anthelmintic activity against chicken tapeworm were conducted. Leave of painted nettle were collected and extracted with hexane, chloroform, ethanol and water. Phytochemical analysis was carried out to determine the chemical compound of secondary metabolites. Anthelmintic activity was evaluated with an assay using chicken tapeworm in a serial microplate dilution method by determination of efective concentration 50 (EC50 using probit analysis. The result of phytochemistry analysis showed that Coleus leaves consisted of flavonoid, steroid, tannin and saponin. Three of four extracts displayed strong anthelmintic activity with the higest activity belong to chloroform extract with EC50 5 mg/ml followed by n-hexane 9 mg/ml and metanol extract 10,2 mg/ml, while water extract has a weak anthelmintic activity with 106,2 mg/ml. In general, chloroform extract proved to be a more efficient extractant of biologically active compounds than either hexane, ethanol or water extract. The promising activity displayed by a number of extracts has led to further investigation of the active compound. Unfortunately, the result of invivo assay showed that the chloroform extract treatment with dose level 25 mg/kg BW could not to reduce the number of tapeworm in chicken. It is interesting for further investigate the differences of respon between in vitro and in vivo to determine involved factors.

  13. Essential Oils of Phoebe angustifolia Meisn., Machilus velutina Champ. ex Benth. and Neolitsea polycarpa Liou (Lauraceae from Vietnam #

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    Tran D. Thang

    2013-05-01

    Full Text Available The essential oils of the leaves of Phoebe angustifolia Meisn, Machilus velutina Champ. ex Benth and Neolitsea polycarpa H. Liu., were analyzed by gas chromatography (GC and gas chromatography coupled with mass spectrometry (GC-MS. The major compound found in the oils of Phoebe angustifolia were n -hexacadecanoic acid (13.0%, spathulenol (17.0%, sabinene (6.0%, artemisia triene (5.1% and bicyclogermacrene (5.9%. Appreciable quantities of ( E -β-ocimene (9.5%, ( Z -β-ocimene (8.2%, germacrene D (6.8%, allo-ocimene (6.4%, α-phellandrene (5.9%, β-caryophyllene and bicyclogermacrene (ca 5.5% could be identified from Machilus velutina . However, we have identified ( E -β-ocimene (85.6% as the singly abundant constituent of Neolitsea polycarpa with significant amounts of limonene (6.5%. Apart from allo-ocimene (1.8% and spathulenol (1.1%, the other nineteen compounds were identified in amount less than 1%. This is the first comprehensive report on the volatile oils of the studied species.

  14. IDENTIFIKASI MOLEKULER BROAD BEAN WILT VIRUS 2 (BBWV2 DAN CYMBIDIUM MOSAIC VIRUS (CYMMV ASAL TANAMAN NILAM (POGOSTEMON CABLIN BENTH.

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    Miftakhurohmah .

    2016-03-01

    Full Text Available Molecular identification Broad Bean Wilt Virus 2 (BBWV2 and Cymbidium Mosaic Virus (CymMV from patchouli plant (Pogostemon cablin Benth.. Several viruses have been reported to be associated with mosaic disease on patchouli plant in Indonesia. This study aims to identify the two viruses in patchouli cultivation in West Java by studying the molecular characterization. Mosaic symptomatic leaf samples taken from patchouli cultivation in Manoko (Bandung Barat District, West Java Province. RNA extraction was performed using Xprep Plant RNA mini kit. RNA amplification with RT-PCR technique using primers for the cp gene region of BBWV2 and CymMV. The PCR product was sent to PT. Science Genetics Indonesia to do sequencing, then analyzed nucleotide sequences. Results of RT-PCR were performed successfully obtained DNA bands with size accordance with the predictions of the primer design for BBWV2 and CymMV cp region. Further, based on nucleotide and amino acid sequence analyses, the two virus isolates were confirmed as BBWV2 and CymMV respectively. Phylogenetic analyses revealed that BBWV2 Manoko clustered with BBWV2 from Singapore (original host of Brazilian red-cloak, China (pepper and South Korea (chili. Whereas, CymMV Manoko become one cluster with CymMV from India (Phaius sp., Indonesia (Dendrobium, China (vanilla, Thailand (Oncidium, Hawai (Dendrobium and South Korea Cymbidium.

  15. Antidiabetic activity and phytochemical screening of extracts of the leaves of Ajuga remota Benth on alloxan-induced diabetic mice.

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    Tafesse, Tadesse Bekele; Hymete, Ariaya; Mekonnen, Yalemtsehay; Tadesse, Mekuria

    2017-05-02

    Ajuga remota Benth is traditionally used in Ethiopia for the management of diabetes mellitus. Since this claim has not been investigated scientifically, the aim of this study was to evaluate the antidiabetic effect and phytochemical screening of the aqueous and 70% ethanol extracts on alloxan-induced diabetic mice. After acute toxicity test, the Swiss albino mice were induced with alloxan to get experimental diabetes animals. The fasting mean blood glucose level before and after treatment for two weeks in normal, diabetic untreated and diabetic mice treated with aqueous and 70% ethanol extracts were performed. Data were statistically evaluated by using Statistical Package for the Social Sciences software version 20. P-value Phytochemical screening of both extracts indicated the presence of phenolic compounds, flavonoids, saponins, tannins, and steroids, which might contribute to the antidiabetic activity. The extracts, however, did not contain alkaloids and anthraquinones. The aqueous extract (500 mg/kg) showed the highest percentage reduction in blood glucose levels and the ability of A. remota extracts in reducing blood glucose levels presumably due to the presence of antioxidant constituents such as flavonoids. The effect of the extract supported the traditional claim of the plant.

  16. Technological development of aqueous extracts from Calycophyllum spruceanum (Benth. Hook. f. ex K. Schum., Rubiaceae, (mulateiro using factorial design

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    Leidyana M. da Costa

    2011-03-01

    Full Text Available The aim of the present work is to characterize the vegetal raw material of the Calycophyllum spruceanum (Benth. Hook. f. ex K. Schum., Rubiaceae, known as "mulateiro", and to evaluate the influence of extractive parameters for attainment of standardized aqueous extractive solutions. The physical-chemical characterization of the samples was performed using pharmacopoeic and not pharmacopoeic methodologies. A 2³ factorial design was used to evaluate the influence of extraction techniques (infusion/decoction, drug: solvent ratio (2.5 and 7.5%, and extraction time (5 and 15 min on the total tannin content of aqueous extractive solutions from C. spruceanum. The extractive solution that showed higher total tannin and dry residue content had their physical-chemical characteristics determined. The results suggest that an aqueous extractive solution from rinds of C. spruceanum barks with higher tanning yield (9.9 g%, must be standardized using decoction as extraction methodology, with 2.5% of vegetal drug for an extraction time of 15 min. The results of the physical-chemical characterization suggest that environmental factors modify the properties of this species and, therefore, they can influence the quality and security of a product derived from this medicinal plant.

  17. Total Flavonoids from Clinopodium chinense (Benth. O. Ktze Protect against Doxorubicin-Induced Cardiotoxicity In Vitro and In Vivo

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    Rong Chang Chen

    2015-01-01

    Full Text Available Doxorubicin has cardiotoxic effects that limit its clinical benefit in cancer patients. This study aims to investigate the protective effects of the total flavonoids from Clinopodium chinense (Benth. O. Ktze (TFCC against doxorubicin- (DOX- induced cardiotoxicity. Male rats were intraperitoneally injected with a single dose of DOX (3 mg/kg every 2 days for three injections. Heart samples were collected 2 weeks after the last DOX dose and then analyzed. DOX delayed body and heart growth and caused cardiac tissue injury, oxidative stress, apoptotic damage, mitochondrial dysfunction, and Bcl-2 expression disturbance. Similar experiments in H9C2 cardiomyocytes showed that doxorubicin reduced cell viability, increased ROS generation and DNA fragmentation, disrupted mitochondrial membrane potential, and induced apoptotic cell death. However, TFCC pretreatment suppressed all of these adverse effects of doxorubicin. Signal transduction studies indicated that TFCC suppressed DOX-induced overexpression of p53 and phosphorylation of JNK, p38, and ERK. Studies with LY294002 (a PI3K/AKT inhibitor demonstrated that the mechanism of TFCC-induced cardioprotection also involves activation of PI3K/AKT. These findings indicated the potential clinical application of TFCC in preventing DOX-induced cardiac oxidative stress.

  18. Assessment of the cytotoxicity and genotoxicity properties of Uvaria chamae P. Beauv (Annonaceae) and Morinda lucida Benth (Rubiaceae) in mice.

    Science.gov (United States)

    Awodiran, M O; Adepiti, A O; Akinwunmi, K F

    2017-08-31

    The toxicity profile of medicinal plants is an important preclinical requirement in the development of phytomedicines. The cytotoxic and genotoxic effects of the leaf of Uvaria chamae P. Beauv (Annonaceae) and stem bark of Morinda lucida Benth (Rubiaceae) were investigated in order to provide information on their safety as antimalarial plants. The methanol extract of U. chamae and ethanol (70%) extract of M. lucida were separately orally administered (125, 250, and 750 mg/kg/day) to mice for 10 consecutive days. Cyclophosphamide (50 mg/kg, single dose) and distilled water were used as positive and negative controls, respectively. The mice were injected with colchicine (0.04%) intra-peritoneally 24 h after the last administration of the extracts and the bone marrows harvested. Giemsa-stained slides of bone marrow cells were microscopically assessed for dividing cells to determine the mitotic index (MI) and scored for chromosomal aberrations (CA) according to standard methods. chamae exhibited dose-dependent cytotoxicity. At 750 mg/kg, the MI was significantly (p lucida was not significantly different (p > 0.05) from that of the negative control. The total CA observed from treatment with both plants at all doses were significantly (p lucida exerted only genotoxic effect. Nevertheless, the two plants should be used with caution in antimalarial therapy.

  19. Alternative formation of anthraquinones and lipoquinones in heterotrophic and photoautotrophic cell suspension cultures of Morinda lucida Benth.

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    Igbavboa, U; Sieweke, H J; Leistner, E; Röwer, I; Hüsemann, W; Barz, W

    1985-12-01

    Photoheterotrophic and photoautotrophic cell suspension cultures were raised from a callus tissue derived from a Morinda lucida Benth. plant (Rubiaceae). The cultures were characterized with regard to fresh weight, dry weight, cell number, pH, chlorophyll and quinoid natural products. The amount of lipoquinones (phylloquinone, α-tocopherol, plastoquinone, ubiquinone) isolated from the photoautotrophic cultures matched the amount detected in an intact leaf. Anthraquinone glycosides which are found in the roots of Morinda plants were not present in the photoautotrophic culture. The photoheterotrophic culture contained only trace amounts of these pigments. Abundant anthraquinone synthesis was observed when photoautotrophic and photoheterotrophic suspension cultures were transferred into darkness, provided sucrose was present in the medium. Induction of synthesis of anthraquinone pigments coincided with a rapid disappearance of lipoquinones from the culture. Thus, in the suspension culture, photoautotrophy correlates with lipoquinone synthesis and heterotrophy correlates with anthraquinone synthesis. This reflects the situation in the intact plants where lipoquinones are chloroplast-associated whereas anthraquinones occur in the roots.

  20. HPLC and GC-MS Determination of Bioactive Compounds in Microwave Obtained Extracts of Three Varieties of Labisia pumila Benth.

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    Hawa Z.E. Jaafar

    2011-08-01

    Full Text Available Microwave extraction of phytochemicals from medicinal plant materials has generated tremendous research interest and shown great potential. This research highlights the importance of microwave extraction in the analysis of flavonoids, isoflavonoid and phenolics and the antioxidant properties of extracts from three varieties of the Malaysian medicinal herb, Labisia pumila Benth. High and fast extraction performance ability, equal or higher extraction efficiencies than other methods, and the need for small samples and reagent volumes are some of the attractive features of this new promising microwave assisted extraction (MAE technique. The aims of the present research were to determine the foliar phenolics and flavonoids contents of extracts of three varieties of L. pumila obtained by a microwave extraction method while flavonoid, isoflavonoid and phenolic compounds were analyzed using RP-HPLC. Furthermore, the antioxidant activities were measured by the DPPH and FRAP methods and finally, the chemical composition of the crude methanolic extracts of the leaves of all three varieties were analyzed by GS-MS.

  1. Gastroprotection of Suaveolol, Isolated from Hyptis suaveolens, against Ethanol-Induced Gastric Lesions in Wistar Rats: Role of Prostaglandins, Nitric Oxide and Sulfhydryls

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    María Elena Sánchez-Mendoza

    2012-07-01

    Full Text Available Hyptis suaveolens is a medicinal plant that is, according to traditional medicine, considered useful in the treatment of gastric ulcers. Although its gastroprotective activity was reported, the active compounds have not been identified. Therefore, the aim of the present study was to identify at least one active compound potentially responsible for the gastroprotective activity of H. suaveolens by using a bioassay guided study with an ethanol-induced gastric ulcer experimental model in rats. The results show that the hexane extract had protective activity (close to 70% when using doses between 10 and 100 mg/kg, and that the compound suaveolol, isolated from this extract, was one of the active gastroprotective agents. This is the first report about the gastroprotective activity of suaveolol. Rats treated with this compound at 3, 10, 30 and 100 mg/kg showed 12.6, 21.3, 39.6 and 70.2% gastroprotection respectively. The effect elicited by suaveolol (at 100 mg/kg was attenuated by pretreatment with either NG-nitro-L-arginine methyl ester (70 mg/kg, i.p., a nitric oxide (NO synthase inhibitor, indomethacin (10 mg/kg, s.c., a blocker of prostaglandin synthesis, or N-ethylmaleimide (10 mg/kg, s.c., a blocker of sulfhydryl groups. This suggests that the gastroprotective mechanism of action of this compound involves NO, prostaglandins and sulfhydryl groups.

  2. Activity studies of sesquiterpene oxides and sulfides from the plant Hyptis suaveolens (Lamiaceae) and its repellency on Ixodes ricinus (Acari: Ixodidae).

    Science.gov (United States)

    Ashitani, T; Garboui, S S; Schubert, F; Vongsombath, C; Liblikas, I; Pålsson, K; Borg-Karlson, A-K

    2015-12-01

    Hyptis suaveolens (Lamiaceae), a plant traditionally used as a mosquito repellent, has been investigated for repellent properties against nymphs of the tick Ixodes ricinus. Essential oils and volatile compounds of fresh and dried leaves, from plants originating from Laos and Guinea-Bissau, were identified by GC-MS and tested in a tick repellency bioassay. All the essential oils were strongly repellent against the ticks, even though the main volatile constituents differed in their proportions of potentially tick repellent chemicals. (+)/(-)-sabinene were present in high amounts in all preparations, and dominated the emission from dry and fresh leaves together with 1,8-cineol and α-phellandrene. 1,8-Cineol and sabinene were major compounds in the essential oils from H. suaveolens from Laos. Main compounds in H. suaveolens from Guinea-Bissau were (-)-sabinene, limonene and terpinolene. Among the sesquiterpene hydrocarbons identified, α-humulene exhibited strong tick repellency (96.8 %). Structure activity studies of oxidation or sulfidation products of germacrene D, α-humulene and β-caryophyllene, showed increased tick repellent activity: of mint sulfide (59.4 %), humulene-6,7-oxide (94.5 %) and caryophyllene-6,7-oxide (96.9 %). The substitution of oxygen with sulfur slightly lowered the repellency. The effects of the constituents in the oils can then be regarded as a trade off between the subsequently lower volatility of the sesquiterpene derivatives compared to the monoterpenes and may thus increase their potential usefulness as tick repellents.

  3. Comparative toxicity effect of bush tea leaves (Hyptis suaveolens) and orange peel (Citrus sinensis) oil extract on larvae of the yellow fever mosquito Aedes aegypti.

    Science.gov (United States)

    Amusan, A A S; Idowu, A B; Arowolo, F S

    2005-09-01

    The ethanolic extracts of the orange peel (Citrus sinensis) and bush tea leaves (Hyptis suaveolens) were compared for their toxicity effect on the larvae of the yellow fever mosquito Aedes aegypti collected from disused tyres beside College of Natural Sciences building University of Agriculture, Abeokuta, Nigeria. Eight graded concentrations, 0.9ppm, 0.8ppm, 0.7ppm, 0.6ppm, 0.5ppm, 0.4ppm, 0.3ppm and 0.2ppm of both plant extracts were tested on the larvae. The mean lethal dose LD10, was 0.15 ppm for C. sinensis, 0.01 for H. suaveolens, while LD50 for C. sinensis was 0.4ppm, H. suaveolens 0.60ppm and LD90 for C. sinensis was 0.9ppm and H. suaveolens was 1.45ppm. LD10 for the control 0.65ppm, LD50 0.9ppm and LD90 2.0 ppm. The extract of C. sinensis peel caused higher mortality rate at concentrations 0.8ppm (95%) and 0.3ppm (90%) of the larvae while the extract of H. suaveolens caused high mortality rate on the larvae at concentrations of 0.9ppm (80%) and 0.3ppm (80%). Significant differences were observed between untreated and treated larvae (exposed to either of the extract) at the various concentrations (P< 0.05).

  4. Gastroprotection of suaveolol, isolated from Hyptis suaveolens, against ethanol-induced gastric lesions in Wistar rats: role of prostaglandins, nitric oxide and sulfhydryls.

    Science.gov (United States)

    Vera-Arzave, Carlos; Antonio, Leticia Cruz; Arrieta, Jesús; Cruz-Hernández, Gerardo; Velasquez-Mendez, Antonio Magdiel; Reyes-Ramírez, Adelfo; Sánchez-Mendoza, María Elena

    2012-07-26

    Hyptis suaveolens is a medicinal plant that is, according to traditional medicine, considered useful in the treatment of gastric ulcers. Although its gastroprotective activity was reported, the active compounds have not been identified. Therefore, the aim of the present study was to identify at least one active compound potentially responsible for the gastroprotective activity of H. suaveolens by using a bioassay guided study with an ethanol-induced gastric ulcer experimental model in rats. The results show that the hexane extract had protective activity (close to 70% when using doses between 10 and 100 mg/kg), and that the compound suaveolol, isolated from this extract, was one of the active gastroprotective agents. This is the first report about the gastroprotective activity of suaveolol. Rats treated with this compound at 3, 10, 30 and 100 mg/kg showed 12.6, 21.3, 39.6 and 70.2% gastroprotection respectively. The effect elicited by suaveolol (at 100 mg/kg) was attenuated by pretreatment with either N(G)-nitro-L-arginine methyl ester (70 mg/kg, i.p.), a nitric oxide (NO) synthase inhibitor, indomethacin (10 mg/kg, s.c.), a blocker of prostaglandin synthesis, or N-ethylmaleimide (10 mg/kg, s.c.), a blocker of sulfhydryl groups. This suggests that the gastroprotective mechanism of action of this compound involves NO, prostaglandins and sulfhydryl groups.

  5. Screening of the odour-activity and bioactivity of the essential oils of leaves and flowers of Hyptis Passerina Mart. from the Brazilian Cerrado

    Energy Technology Data Exchange (ETDEWEB)

    Zellner, Barbara D.; Amorim, Ana Carolina L.; Rezende, Claudia M. [Universidade Federal do Rio de Janeiro (UFRJ), RJ (Brazil). Centro de Tecnologia. Inst. de Quimica]. E-mail: crezende@iq.ufrj.br; Miranda, Ana Luisa P. de [Universidade Federal do Rio de Janeiro (UFRJ), RJ (Brazil). Faculdade de Farmacia; Alves, Ruy J.V. [Museu Nacional, Rio de Janeiro, RJ (Brazil). Dept. de Botanica. Herbario; Barbosa, Jussara P.; Costa, Gisela L. da [Instituto Oswaldo Cruz (FIOCRUZ), Rio de Janeiro, RJ (Brazil). Lab. de Taxonomia, Bioquimica e Bioprospeccao de Fungos

    2009-07-01

    The chemical profile of the essential oils obtained from the leaves and flowers of Hyptis passerina Mart., a rare species of the Brazilian Cerrado, has been determined for the first time. Analyses by GC-MS showed sesquiterpenes as major compounds. {beta}-epi-acorenol (35.7% and 32.8%, respectively from leaf and flower essential oils), was isolated and identified by 1D and 2D NMR. The flower-derived oil presented a higher concentration of hydrocarbon and oxygenated monoterpenes, while the leaf-oil was richer in diterpenes. The global odour impressions of both oils were given by direct analysis and GC-MS-O and were characterized as herbaceous with tea notes, and green, cooked and woody impressions for leaf-oil; herbaceous, with spicy, woody and minty notes for flower-oil. {beta}-epi-acorenol, spathulenol, {beta}-caryophyllene, and caryophyllene oxide were relevant for the odour-activity of both oils, as well as minor constituents, such as linalool. The antimicrobial activity was investigated by means of agar diffusion disc method and contact bioautography, against Gram-positive and negative bacteria and yeast. Both oils presented to be bioactive against the tested microorganisms with significant inhibition level. (author)

  6. Nematicidal potential of aqueous extract of Hyptis suaveolens in the management of root-knot nematode, Meloidogyne incognita of some cowpea cultivars

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    S. A. Abolusoro

    2016-01-01

    Full Text Available Studies were conducted under field and screenhouse conditions to investigate the potentials of crude aqueous leaf extract of Hyptis suaveolens in the management of the root-knot nematode Meloidogyne incognita of three cowpea varieties (Sampea 9, 10 and 11. A Randomized Complete Block Design was used in the field while a completely randomized design was used for the screenhouse trials. Results showed that the treatment significantly (p < 0.05 improved the growth and yield of the three varieties and also reduced soil nematode population and root galls. It was also observed that all the three varieties were susceptible to the root-knot nematode infestation but Sampea 10 recorded higher yield that were significant in the pot trials. Phytochemical screening revealed the presence of saponins, alkaloids, flavonoids and steroids in the leaves of H. suaveolens. For higher yield of the evaluated cowpea varieties in a nematode endemic zone, aqueous leaf extract of H. suaveolens is being recommended for infested soil treatment.

  7. Evaluation of glucose utilization capacity of bioactivity-guided fractions of Barleria prionitis Linn and Hyptis suaveolens (L.) Poit in isolated rat hemidiaphragm

    Science.gov (United States)

    Begum, Azmath Unnisa; Venkatesh, Sama; Prakash, Jaya; Alvala, Ravi

    2016-01-01

    Introduction: Diabetes mellitus (DM) is a chronic disease characterized by high blood glucose levels due to absolute or relative circulating insulin levels. Plants represent a major potential source of drugs for treating diabetes. The study of plants having antidiabetic activity may give a new approach in the treatment of DM. Aim: This study was aimed to investigate the glucose utilization capacity of bioactivity-guided fractions of Barleria prionitis and Hyptis suaveolens. Materials and Methods: The dried coarse powdered plant material was extracted in aqueous ethanol by cold maceration; further, ethanolic extracts were fractionated using solvents of varying polarity and were investigated in isolated rat hemidiaphragm using 0.1% and 0.2% concentrations of plant extracts. Results: The tested fractions of both plants showed significant and dose-dependent increased glucose uptake capacity and was found to be maximum with petroleum ether and aqueous ethanolic extracts of both plants; 0.2% concentration of both plant extracts is superior in activity when compared to 0.1% of the test extracts. H. suaveolens has produced more glucose utilization capacity when compared to B. prionitis. Conclusion: The activity of H. suaveolens is comparable to standard insulin (P < 0.01). Both the plant materials have some extra pancreatic mechanism like glucose uptake by peripheral tissue. PMID:29200754

  8. Coordination polymer adsorbent for matrix solid-phase dispersion extraction of pesticides during analysis of dehydrated Hyptis pectinata medicinal plant by GC/MS.

    Science.gov (United States)

    Aquino, Adriano; Wanderley, Kaline A; Paiva-Santos, Carlos de Oliveira; de Sá, Gilberto F; Alexandre, Marcelo da R; Júnior, Severino A; Navickiene, Sandro

    2010-12-15

    The coordination polymer [Zn(BDC)(H(2)O)(2)](n) was tested for extraction of pyrimethanil, ametryn, dichlofluanid, tetraconazole, flumetralin, kresoxim-methyl and tebuconazole from the medicinal plant Hyptis pectinata, with analysis using gas chromatography-mass spectrometry in selected ion monitoring mode (GC/MS, SIM). Experiments carried out at different fortification levels (0.1, 0.5 and 1.0 μg g(-1)) resulted in recoveries in the range 73-97%, and RSD values were between 5 and 12% for the [Zn(BDC)(H(2)O)(2)](n) sorbent. Detection and quantification limits ranged from 0.02 to 0.07 μg g(-1) and from 0.05 to 0.1 μg g(-1), respectively, for the different pesticides studied. The method developed was linear over the range tested (0.04-14.0 μg g(-1)), with correlation coefficients ranging from 0.9987 to 0.9998. Comparison between [Zn(BDC)(H(2)O)(2)](n) and the commercial phase C(18)-bonded silica showed good performance of the [Zn(BDC)(H(2)O)(2)](n) polymeric sorbent for the pesticides tested. Copyright © 2010 Elsevier B.V. All rights reserved.

  9. Sedative and anesthetic activities of the essential oils of Hyptis mutabilis (Rich.) Briq. and their isolated components in silver catfish (Rhamdia quelen).

    Science.gov (United States)

    Silva, L L; Garlet, Q I; Benovit, S C; Dolci, G; Mallmann, C A; Bürger, M E; Baldisserotto, B; Longhi, S J; Heinzmann, B M

    2013-09-01

    This study evaluated the sedative and anesthetic effects of the essential oils (EO) of Hyptis mutabilis (Rich.) Briq. and their isolated components on silver catfish (Rhamdia quelen). Quantitative chemical differences between the EOs obtained from leaves and inflorescences were verified, and a new chemotype rich in globulol was described. Although there were no significant differences in the time of induction for sedation and anesthesia between the EOs, only the leaf EO at 344 mg/L anesthetized all fish without side effects. Fractionation of the leaf EO was carried out by column chromatography. The isolated compounds [(+)-1-terpinen-4-ol and (-)-globulol] showed different activity from that detected for the leaf EO in proportional concentrations and similar sedation to a eugenol control at 10 mg/L. However, fish exposed to 1-terpinen-4-ol (3 and 10 mg/L) did not remain sedated for 30 min. Anesthesia was obtained with 83-190 mg/L globulol, but animals showed loss of mucus during induction and mortality at these concentrations. Synergism of the depressor effects was detected with the association of globulol and benzodiazepine (BDZ), compared with either drug alone. Fish exposed to BDZ or globulol+BDZ association showed faster recovery from anesthesia in water containing flumazenil, but the same did not occur with globulol. In conclusion, the use of globulol in aquaculture procedures should be considered only at sedative concentrations of 10 and 20 mg/L, and its mechanism of action seems not to involve the GABAA-BDZ system.

  10. Physicochemical characterization and analgesic effect of inclusion complexes of essential oil from Hyptis pectinata L. Poit leaves with β-cyclodextrin.

    Science.gov (United States)

    Menezes, Paula dos P; Araujo, Adriano A de S; Doria, Grace Anne A; Quintans-Junior, Lucindo J; de Oliveira, Makson G B; dos Santos, Marcio R V; de Oliveira, Juliana F; Matos, Jivaldo do R; Carvalho, Flavio M de S; Alves, Pericles B; de Matos, Iara L; dos Santos, Darlisson A; Marreto, Ricardo N; da Silva, Gabriel F; Serafini, Mairim R

    2015-01-01

    The formation of inclusion complexes of Hyptis pectinata essential oil (EOHP), with potent activities such as anti-nociceptive, anti-inflammatory, among others, with β -cyclodextrin (β-CD), was obtained by slurry (SC) and paste procedures (PC). The gas chromatography coupled to the mass spectrometry (GC/MS) analysis demonstrated a total of 36.4% monoterpenes and 63.6% sesquiterpenes in the EOHP. The major components of EOHP were identified as (E)- caryophyllene (54.07%). The analysis of samples (PM, PC and SC) by GC/MS involved the surface and the total extracted oils. The GC/MS results suggested important differences between in SC and PC methods indicating the complexation of mono and sesquiterpenoids in different ratios. Furthermore, the thermal analysis techniques suggests the complexation, especially in SC, which show a thermogravimetry/derivative thermogravimetry (TG/DTG) peak at 140-270ºC, probably related to oil loss. Scanning electron microscopy (SEM) images showed reduction size of the samples mainly in the SC product. Additionally, EOHP/ β-CD improves pharmacological profile of EOHP alone in formalin-induced pain protocol in mice.

  11. Potential use of the bushmint, Hyptis suaveolens, for the control of infestation by the pink stalk borer, Sesamia calamistis on maize in southern Benin, West Africa.

    Science.gov (United States)

    Adda, Cyrille; Atachi, Pierre; Hell, Kerstin; Tamò, Manuele

    2011-01-01

    Maize production in Benin, especially in resource-poor farmers' fields, is constrained by stemborers among other factors. One of the major stemborers in southern Benin is Sesamia calamistis Hampson (Lepidoptera: Noctuidae). African farmers cannot afford to use commercial insecticides for controlling stemborers - they are expensive and unsuitable for durable pest management systems due to eco-toxicity. There is therefore a need for cheaper and environmentally friendly methods and botanicals offer an attractive alternative. The bushmint, Hyptis suaveolens (L.) Poit. (Lamiales: Lamiaceae), was compared with the commercial insecticide Furadan (carbofuran) for the control of S. calamistis on maize Zea mays L. (Poales: Poaceae). Trials were conducted in the screenhouse and in the field during the minor cropping season in 2004 at the International Institute of Tropical Agriculture (IITA)-Benin station. The variables measured included numbers of egg masses per plant, eggs per egg mass (in the screenhouse study), population density of S. calamistis, percentage of infested plants and/or ears, and deadhearts in the field. Irrespective of the variable considered, the aqueous extract of H. suaveolens compared favorably with Furadan while maize surrounded by live H. suaveolens plants had lower S. calamistis densities.

  12. Nanoemulsion Thermoreversible Pluronic F127-Based Hydrogel Containing Hyptis pectinata (Lamiaceae) Leaf Essential Oil Produced a Lasting Anti-hyperalgesic Effect in Chronic Noninflammatory Widespread Pain in Mice.

    Science.gov (United States)

    Quintans-Júnior, Lucindo J; Brito, Renan G; Quintans, Jullyana S S; Santos, Priscila L; Camargo, Zaine T; Barreto, Péricles A; Arrigoni-Blank, Maria F; Lucca-Júnior, Waldecy; Scotti, Luciana; Scotti, Marcus T; Kolker, Sandra J; Sluka, Kathleen A

    2017-02-13

    We evaluated if a nanostructured thermoreversible Pluronic F127-based hydrogel incorporated with Hyptis pectinata leaf essential oil (NE-EOH) produces a long-lasting anti-hyperalgesic effect on chronic muscle pain in an animal model. We induced chronic muscle pain by injecting the gastrocnemius with saline injections. Paw and muscle withdrawal thresholds and motor performance were evaluated after treatment and compared with morphine, diazepam, or vehicle. Naloxone and methysergide administration tested the involvement of opioid and serotonin receptors, respectively. Sites of action in the central nervous system for the NE-EOH were examined by measuring substance P (SP) levels in the spinal cord and Fos protein in the brainstem. NE-EOH increased paw and muscle withdrawal thresholds when compared with vehicle but had no effect on motor function. This analgesic effect was reversed by both naloxone and methysergide. NE-EOH decreased elevated substance P levels and reduced Fos-labeled neurons in the spinal cord and increased the number of Fos-labeled neurons in the periaqueductal gray (PAG), nucleus raphe magnus (NRM), and locus coeruleus (LC). NE-EOH was shown to produce a lasting anti-hyperalgesic effect. It uses opioid and serotonin receptors, activates brainstem inhibitory pathways, and reduces the release of excitatory neurotransmitters in the spinal cord and is a substance with potential to be used in the treatment of noninflammatory pain conditions. Graphical Abstract.

  13. Evaluation of the orofacial antinociceptive profile of the ethyl acetate fraction and its major constituent, rosmarinic acid, from the leaves of Hyptis pectinata on rodents

    Directory of Open Access Journals (Sweden)

    Rosângela E.A. Falcão

    Full Text Available ABSTRACT Hyptis pectinata (L. Poit., Lamiaceae, popularly known as "sambacaitá," is an aromatic shrub largely grown in the Brazilian northeastern. We investigated the antinociceptive effects of the ethyl acetate fraction obtained from the leaves of H. pectinata and of its main constituent rosmarinic acid, on formalin (2%-, glutamate (25 µM- and capsaicin (2.5 µg-induced orofacial nociception in rodents. Male mice were pretreated with ethyl acetate fraction (100, 200 or 400 mg/kg, p.o., rosmarinic acid (10 or 20 mg/kg, p.o., morphine (5 mg/kg, i.p., or vehicle (distilled water + 0.2% Tween 80. Ethyl acetate fraction reduced the nociceptive face-rubbing behavior during the two phase of the formalin test, whereas pretreatment with rosmarinic acid decreased the pain behavior in the second phase. Ethyl acetate fraction produced significant antinociceptive effects in the capsaicin and glutamate tests. This study showed that oral administration of ethyl acetate fraction produced potent antinociceptive effects compared to treatment with rosmarinic acid.

  14. 5-H¡droxi-2-Metil-1,4-Naftoquinona obtenida de Pera nítida (Benth. Jablonski(Euforbiaceae

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    Luis E. Cuca S.

    2009-07-01

    Full Text Available Del extracto clorofórmico de la corteza del tronco de Pera nítida (Benth. Jablonski se aisló una sustancia amarilla que fue identificada como 5—hidro.x¡-2— metil —1,4—naftoquinona. Esta sustancia es conocida como plumbagina y ha mostrado una destacada acción biológica. La estructura fue deducida con base en los datos espectroscópicos y su presencia en esta especie tiene importancia quimiotaxonómica.

  15. Respuesta al estrés por limitación de nitrógeno de plántulas de castilleja tenuiflora benth. cultivadas in vitro.

    OpenAIRE

    Cortes Morales, Jorge Alberto

    2016-01-01

    Castilleja tenuiflora Benth. (Orobanchaceae), es una planta medicinal silvestre que sintetiza diversos compuestos químicos como feniletanoides e iridoides glicosilados y flavonoides. El perfil químico de los cultivos in vitro de C. tenuiflora varía dependiendo de factores bióticos y de factores abióticos como el nitrógeno. El objetivo general de este trabajo fue evaluar la respuesta al estrés por limitación de nitrógeno de plántulas de C. tenuiflora cultivadas in vitro. Se cult...

  16. Crescimento inicial de mudas de pau ferro (Caesalpinia ferreaMart. ex Tul. var. leiostachya Benth. sob diferentes regimes hídricos Initial growth of wood iron (Caesalpinia ferrea Mart. ex. Tul var. leiostachya Benth under different hydric regimes

    Directory of Open Access Journals (Sweden)

    Nádia Regina Lenhard

    2010-08-01

    Full Text Available Objetivou-se, com este estudo avaliar o crescimento inicial de mudas de pau-ferro (Caesalpinia ferrea Mart. ex. Tul. var. leiostachya Benth -Fabaceae-Caesalpinioideae sob quatro regimes hídricos (alagamento, 70%, 40% e 12,5% de capacidade de campo. As mudas foram submetidas aos tratamentos aos 60 dias de idade. O delineamento experimental foi inteiramente casualizado, com quatro repetições de oito mudas por tratamento. Após 7 dias de permanência sob tratamento, iniciaram-se as avaliações. O diâmetro (6,5 mm, teor relativo de água (52,45%, área foliar específica (174,58 g cm², taxa de crescimento relativo (0,0160 g g-1, taxa assimilatória líquida (0,0004 g dm-2 dias-1 e peso específico de folha (0,0062 g cm² não variaram estatisticamente entre os tratamentos. As mudas sob 70% CC apresentaram maior altura (84,6cm, tamanho da raiz (28,9 cm, massa seca da raiz (6,24 g, área foliar (376,0 cm², massa seca da folha (1,98 g, massa seca da parte aérea (8,27 g e teor de clorofila (31,67 ìg cm². A razão de peso específico (0,2368 g g-1 e o teor de nitrogênio (0,37 g kg-1 foram maiores sob 12,5% CC. A razão de área foliar (35,09 g g-1 foi menor sob 12,5% CC. Dessa forma, a melhor condição para a produção das mudas é em 70% da capacidade de campo.The objective of this study was to evaluate the initial growth of Caesapinia ferrea Mart ex. Tul var. leiostachva Benth (Fabaceae-Caesalpinioideae under four hidric regimes (overflow, 70, 40 and 12.5% of field capacity. The seedlings were submitted to the treatments after 60 days of age. The experimental delineation was completely randomized with four repetitions of eight seedlings for treatment. After 7 days of permanence under treatment, the evaluations had been initiated. The diameter (6,5 mm, relative water content (52.45%, specific foliar area (174.58 g cm², rate of relative growth (0.0160 g g-1, liquid assimilated rate (0.0004 g dm-2 days-1 and specific weight of leaf (0.0062 g

  17. Germinação de sementes de dalbergia nigra (Vell. Allemao ex Benth. (Fabaceae-Papilionoideae no armazenamento Germination of Dalbergia nigra (Vell. Allemao ex Benth. (Fabaceae-Papilionoideae during storage

    Directory of Open Access Journals (Sweden)

    Francismar Francisco Alves Aguiar

    2010-12-01

    Full Text Available Dalbergia nigra (Vell. Allemao ex Benth. (jacarandá-da-bahia, espécie de alto valor econômico pela qualidade da madeira e potencial ornamental em razão da copa bem formada e à delicadeza da folhagem, está na lista de espécies ameaçadas de extinção. Em função disso, conduziu-se este trabalho, visando à avaliação da qualidade de suas sementes, submetidas a períodos crescentes de armazenamento. Os frutos foram coletados em outubro de 2003, e as sementes armazenadas em geladeira a 6º C ± 2, em sacos de plástico transparente fechados. Mensalmente, durante 690 dias, foram retiradas 200 sementes, sendo que 100 foram utilizadas nos ensaios de germinação (quatro repetições de 25 sementes e 100 para a determinação do teor de água. Os ensaios germinativos foram conduzidos em estufa tipo B.O.D., a 25º C e com fotoperíodo de 12 horas. As variáveis analisadas foram a porcentagem de germinação, índice de velocidade de germinação e massa seca das plântulas originadas. Os resultados indicaram que, até 360 dias de armazenamento, as sementes apresentaram porcentagem de germinação próxima de 50%, com redução de cerca da metade do índice de velocidade de germinação, porém, com maior comprometimento do acúmulo da massa seca das plântulas. A porcentagem de germinação inicial e final foram de 87% e 36%, respectivamente. As sementes de D. nigra têm comportamento ortodoxo e podem ser armazenadas por um período de até dois anos em sacos de plástico a baixa temperatura, com redução em cerca de 50% de sua capacidadeDalbergia nigra (Vell. Allemao ex Benth. (Brazilian rosewood is a highly economically valuable species because of its wood quality and ornamental potential, mainly due to its well formed canopy and the delicacy of its foliage. Unfortunately, these features contributed to list it as an endangered Brazilian species (IBAMA Regulation number 06/92. This work had as its objective to study the quality of Dalbergia

  18. Composition chimique et propriétés physico-chimiques des huiles essentielles d’Ocimum basilicum et d’Hyptis suaveolens (L.) Poit. récoltés dans la région de Dakar au Sénégal

    National Research Council Canada - National Science Library

    Jean Michel Kornprobst; Abdoulaye Samb; Fatou Dieng Faye; Moussoukhoye Diop; Saliou Ngom

    2013-01-01

    Les huiles essentielles des feuilles et des fleurs d’Ocimum basilicum et d’Hyptis suaveolens (deux espèces de Lamiacées), récoltées dans la région de Dakar au Sénégal ont été extraites...

  19. Lowering blood pressure effect of Apium graviolens (seledri and Orthosipleon stamineus benth (kumis kucing in mild and moderate hypertension

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    Siti Supari

    2002-12-01

    Full Text Available In Indonesia Apium graviolens and Orthosiphon stamineus benth have been used to as traditional medicines to lower blood pressure. Since at present time is avaiLable as a combination of those phytopharmaca in the market, therefore, it is necessary to conduct a study to evaLtnte the ffictivity and side effects of those phytopharmaca toward hypertensive subjects. A randomized double blind conftolled trial was conducted at area of Monica - Jakarta in South of Jalarta from July-untiL 29 October 2001 toward mild and moderate hypertensive subjects. The first group (72 subjects received phytophanmaca (Tensigard® /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Table Normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-qformat:yes; mso-style-parent:""; mso-padding-alt:0in 5.4pt 0in 5.4pt; mso-para-margin-top:0in; mso-para-margin-right:0in; mso-para-margin-bottom:10.0pt; mso-para-margin-left:0in; line-height:115%; mso-pagination:widow-orphan; font-size:11.0pt; font-family:"Calibri","sans-serif"; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-fareast-font-family:"Times New Roman"; mso-fareast-theme-font:minor-fareast; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin; mso-bidi-font-family:"Times New Roman"; mso-bidi-theme-font:minor-bidi;} which contains Apium graviolens and Orthosiphon stamineus benth 3 x 250 mg, whiLe the second grottp (70 subjects had Amlodipine I x 5 mg. Obseruation were conducted for 12 weelcs, and the parameter observed wère systolic and diastolic blood pressure (SBP and DBP, plasma lipid, blood glucose before and after treatment, and electrolyte with nvo week intervals. The Resuhs revealed that the phytopharmaca treatment for 12 weel 0,05; DBP 10.00 t0.96 mmHg vs 9.49 t 1.37 ntmHg; P> 0.05. Phytopharmaca treatment for 12

  20. C15083. Chemical Composition and Allelopathic Potential of Essential Oils from Tipuana tipu (Benth.) Kuntze Cultivated in Tunisia.

    Science.gov (United States)

    El Ayeb-Zakhama, Asma; Sakka-Rouis, Lamia; Bergaoui, Afifa; Flamini, Guido; Jannet, Hichem Ben; Harzallah-Skhiri, Fethia

    2016-02-10

    In Tunisia, Tipuana tipu (Benth.) Kuntze is an exotic tree, which was introduced many years ago and planted as ornamental street, garden, and park tree. The present work reported, for the first time, the chemical composition and evaluates the allelopathic effect of the hydrodistilled essential oils of the different parts of this tree, viz., roots, stems, leaves, flowers and pods gathered in the area of Sousse, a coastal region, in the East of Tunisia. In total, 86 compounds representing 89.9-94.9% of the whole oil composition, were identified in these oils by GC-FID and GC/MS analyses. The root essential oil was clearly distinguished for its high content in sesquiterpene hydrocarbons (β-caryophyllene, 1 (44); 24.1% and germacrene D, 2 (53); 20.0%), while those obtained from pods, leaves, stems and flowers were dominated by non-terpene hydrocarbons. The most important ones were n-tetradecane (41, 16.3%, pod oil), 1.7-dimethylnaphthalene (43, 15.6%, leaf oil), and n-octadecane (77, 13.1%, stem oil). The leaf oil was rich in the apocarotene (E)-β-ionone (4 (54); 33.8%), and the oil obtained from flowers was characterized by hexahydrofarnesyl acetone (5 (81); 19.9%) and methyl hexadecanoate (83, 10.2%). Principal component and hierarchical cluster analyses separated the five essential oils into 3 groups and 2 subgroups, each characterized by the major oil constituents. Contact tests showed that the germination of lettuce seeds was totally inhibited by the root essential oil tested at 1 mg/ml. The inhibitory effect on the shoot and root elongation varied from -1.6 to -32.4%, and from -2.5 to -64.4%, respectively. This article is protected by copyright. All rights reserved. This article is protected by copyright. All rights reserved.

  1. Rattle tree (Albizia lebbeck Benth. effects on potato (Solanum tuberosum L. productivity on the Jos Plateau, Nigeria

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    Kareem Alasi Ibraheem

    2014-01-01

    Full Text Available Field experiment was conducted in the biotite-granite area (Alfisol of the Jos Plateau, Nigeria, consisting of five cropping seasons to determine the effects of the tree rows (under alley cropping and green manure of Albizia lebbeck Benth. (rattle tree on the yield / productivity of Solanum tuberosum L. (Irish potato. The experiment was a randomized complete block design comprising five treatments and three replicates. An early maturing potato variety (Bertita was employed, its pre-sprouted tubers were planted in the alleys (spaces between the tree rows of A. lebbeck two weeks after green manure was applied (5 and 10 t ha-1, pre and post experimental soil sample analyses and cultivation operations were carried out. Results indicated that the rattle tree had profound effect on the potato growth parameters (plant height, leaf count, collar girth (at P < 0.01 and yield indices (tuber count (P < 0.05 and tuber weight (P < 0.01 due to nutrients from the green manure and nitrogen fixation activities of the rattle tree. A. lebbeck green manure application at 10 t ha-1 in the alleys of A. lebbeck hedgerows emerged as the most effective treatment in terms of growth performance and optimal yield. Collar girth and leaf count of the potato accounted for 80.4 - 91.3% of the variation in yield (R2 = 0.804 - 0.913 with collar girth having the highest value (R2 =0.910 in rainy season (rain fed cropping, while leaf count had the highest value (R2=0.913 during the dry season (irrigated cropping.

  2. Cytotoxic activity of acyl phloroglucinols isolated from the leaves of Eucalyptus cinerea F. Muell. ex Benth. cultivated in Egypt

    Science.gov (United States)

    Soliman, Fathy M.; Fathy, Magda M.; Salama, Maha M.; Al-Abd, Ahmed M.; Saber, Fatema R.; El-Halawany, Ali M.

    2014-01-01

    Two acyl phloroglucinol compounds namely; Sideroxylonal B (1) and Macrocarpal A (2) were isolated from the Sideroxylonal-Rich Extract (SRE) of the juvenile leaves of Eucalyptus cinerea; F. Muell. ex Benth cultivated in Egypt. Identification of the isolated compounds was established on the basis of physico-chemical properties and spectral analysis (1D & 2D NMR). The two compounds were isolated for the first time from this species. The SRE alongside with the isolated compounds were tested against three human cancer cell lines; MCF7 (breast carcinoma cell line), HEP2 (laryngeal carcinoma), CaCo (colonic adenocarcinoma) and one type of normal human cell line;10 FS (fibroblast cells). The SRE, (1), and (2) showed cytotoxic activity with IC50 13.6 ± 0.62, 7.2 ± 0.5, 14.8 ± 0.55 μg mL−1 against HEP2 respectively, 11.6 ± 0.47, 4 ± 0.36, 11.4 ± 0.45 μg mL−1 against CaCo, respectively, and 8.6 ± 0.29, 4.4 ± 0.25, and 7.8 ± 0.3 μg mL−1 against MCF7, respectively. Meanwhile, the (SRE) together with (1) and (2) exhibited low cytotoxicity against normal cell line 10 FS, with IC50 55.4 ± 1.4, 43 ± 0.8 and 50.1 ± 1.12 μg mL−1, respectively. The antiprofilerative activity of the tested compounds was evaluated. The cell cycle profile of cells treated with Sideroxylonal-B and Macrocarpal-A indicates possible S-phase specific effects. PMID:24986654

  3. Caracterização farmacognóstica das folhas e sementes de Albizia lebbeck (L. Benth. (Fabaceae

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    Cláudia G. Miranda

    Full Text Available Albizia lebbeck (L. Benth. é popularmente conhecida no Brasil por coração-de-negro. É uma espécie aclimatada nesse país. As sementes dessa espécie são utilizadas no tratamento de diarréia, disenteria e hemorróidas e as folhas, no tratamento de úlceras e nas conjuntivites alérgicas. Este trabalho foi realizado com a finalidade de determinar as características macroscópicas e microscópicas das folhas e sementes dessa planta, uma vez que estas são as partes usadas popularmente. Algumas características de valor na sua diagnose foram apontadas e ilustradas por fotomicrografias. Reações de fitoquímica e a determinação do conteúdo de água e de cinzas foram realizadas no pó das folhas e sementes de A. lebbeck. Saponinas, alcalóides e cumarinas foram detectados nas folhas e sementes; flavonóides foram também identificados nas folhas. O teor de umidade foi 6,78% e 7,91%, respectivamente, para o pó das folhas e sementes. Os valores de cinzas totais e cinzas insolúveis em ácido foram 9,60% e 0,62% para as folhas e 4,13% e 0,21% para as sementes. Os dados obtidos são importantes para o controle de qualidade dessa planta medicinal. O extrato bruto da planta não apresentou atividade antibacteriana.

  4. Caracterização botânica e química de Rudgea viburnoides (Cham. Benth., (Rubiaceae

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    R.M.S. Alves

    Full Text Available Congonha, congonha-de-bugre e bugre são nomes populares atribuídos a várias espécies medicinais, incluindo Rudgea viburnoides (Cham. Benth. (Rubiaceae, cujas folhas são utilizadas na medicina popular como diuréticas, hipotensoras, antireumáticas e depurativas do sangue. Esta espécie vem sendo comercializada como porangaba e utilizada em regimes de emagrecimento, substituindo Cordia ecalyculata Vell. (Boraginaceae. Neste estudo, foram determinadas as características botânicas, fisico-químicas e químicas para R. viburnoides com o objetivo de auxiliar sua identificação taxonômica e o controle de qualidade de produtos preparados com esta planta. Uma amostra referência foi coletada e submetida à caracterização macroscópica, microscópica e organoléptica, determinação de cinzas totais e umidade, prospecção fitoquímica e determinação dos perfis cromatográficos em CCD. Amostras de R. viburnoides podem ser reconhecidas pela epiderme pilosa com marcantes estrias cuticulares, estômatos paracíticos, presença de idioblastos com ráfides no mesofilo e pela configuração em arco dos tecidos vasculares da nervura de primeira ordem, caracteres ausentes em C. ecalyculata. A presença de cistólitos e canais mucilaginosos na medula diferem prontamente C. ecalyculata de R. viburnoides. A análise por CCD evidenciou a presença de flavonóides e saponinas. Amostras adquiridas no comércio e submetidas aos mesmos ensaios permitiram validar os métodos propostos e confirmaram a ampla comercialização de R. viburnoides em Minas Gerais.

  5. Estudo do sistema de reprodução da fava-d'anta (Dimorphandra mollis Benth.

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    A.D.R. Mendes

    2013-01-01

    Full Text Available Entre as plantas nativas de uso medicinal do Cerrado brasileiro encontra-se a fava-d'anta (Dimorphandra mollis Benth com alto potencial econômico por possuir inúmeras potencialidades medicinais e fitoquímicas. A indústria extrai dos frutos os princípios ativos rutina, quercetina, e ramnose, dentre outros, usados na fabricação de medicamentos e cosméticos, principalmente no exterior. O conhecimento do sistema reprodutivo da espécie é fundamental para sua conservação e manejo. O presente trabalho objetivou determinar as características morfométricas das flores e caracterizar o sistema reprodutivo da D. mollis em área de Cerrado inalterada antropomorficamente, no município de Olhos D'água - MG. Para as características morfométricas, diâmetro da flor, comprimento da flor, do ovário, e da antera, foram utilizadas cinco flores em pré-antese. Para a determinação do sistema reprodutivo utilizou-se a razão pólen:óvulo (P:O, em 40 flores. As flores da D. mollis apresentaram comprimento da flor de 3,00 mm, diâmetro da flor de 2,00 mm, comprimento do óvulo de 2,60 mm, comprimento da antera de 1,57 mm, e o número de óvulos e de anteras foram 20 e 5,8, respectivamente. A razão P:O foi 765,030, sendo que esse índice não é afetado pelas características morfométricas. O sistema reprodutivo da D. mollis foi classificado como alógama facultativa.

  6. Insectos plagas y benéficos asociados al cultivo de mora (Rubus glaucus, benth en La Sabana, Madriz, Nicaragua

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    Edgardo Jiménez-Martínez

    2006-12-01

    Full Text Available LA MORA (RUBUS GLAUCUS, BENTH ES UNA PLANTA en proceso de domesticación que se cultiva en pequeñas parcelas. Es hospedera de muchos insectos plaga y enfermedades. En Nicaragua se establecieron plantaciones de mora desde hace unos tres años, como alternativa de diversificación de fincas cafetaleras en los departamentos de Madriz y Nueva Segovia. Hasta ahora no hay información formal sobre los principales insectos plaga y benéficos presentes en este cultivo, que está tomando gran importancia. Por ello, se hizo una investigación para describir la fluctuación poblacional de los insectos plaga y sus depredadores naturales, asociados a este cultivo. El estudio se realizó en la finca La Patasta, municipio La Sabana, departamento de Madriz, entre septiembre de 2004 y abril de 2005. El monitoreo se realizó semanalmente en cinco sitios específicos, mediante capturas manuales de especimenes, con ayuda de bolsas de plástico y vasos de vidrio con alcohol. Como resultado, se identificó y describió la fluctuación poblacional de insectos de las principales familias Scarabaeidae, Chrysomelidae, Curculionidae, Cantharidae, y de los órdenes Hemíptero (Cicadellidae, Pentatomidae, Miridae y Orthóptero (Acrididae y Tettigonidae. Igualmente, se identificó y describió la fluctuación poblacional de depredadores naturales de insectos de la familia Staphilinidae, Coccinelidae, Vespidae y Aracnidae, presentes en este cultivo.

  7. Antidiarrheal activity of 80 % methanol extract of the aerial part of Ajuga remota Benth (Lamiaceae) in mice.

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    Yacob, Teshager; Shibeshi, Workineh; Nedi, Teshome

    2016-08-22

    In the Ethiopian traditional medicine, the aerial part of Ajuga remota Benth is used in the treatment of diarrhea. There are different mechanisms by which Ajuga remota may have antidiarrheal effect. Some of the possible mechanisms are through its anthelmintic and antibacterial activity. The present study aimed to evaluate whether the antidiarrheal effect of the plant also include antimotility and antisecretory effect using 80 % methanol extract of A. remota (MEAR). The MEAR was administered at doses of 200, 400, 600 and 800 mg/kg to four groups of mice (six animals per group) orally in castor oil diarrhea model. The effect of the extract on enteropooling and gastrointestinal transit model was also evaluated using the same grouping and dosing. Two other groups, one as control and the other as standard (loperamide 5 mg/kg) were used for comparison with the treatment groups. The extract at the doses of 400, 600, and 800 mg/kg produced a dose-dependent and significant inhibition both on the frequency and onset of diarrhea. The percentage purging frequency was 53.4, 66.7, 79.6, and 66.7 % (p < 0.001) at three doses of MEAR (400, 600, and 800 mg/kg) and with loperamide (5 mg/kg), respectively. The percentage inhibition in intestinal fluid accumulation was 42.5, 62.1, and 74.2 % (p < 0.001) at the doses of 400, 600 and 800 mg/kg of MEAR, respectively. The MEAR also inhibited the intestinal transit of charcoal meal in a dose dependent manner both in the normal and castor oil induced intestinal transit. This study has shown that the 80 % methanol extract of A. remota contains pharmacologically active substances with significant antimotility and antisecretory effect contributing for its antidiarrheal activity.

  8. The inhibitory potential of the condensed-tannin-rich fraction of Plathymenia reticulata Benth. (Fabaceae) against Bothrops atrox envenomation.

    Science.gov (United States)

    de Moura, Valéria Mourão; da Silva, Wania Cristina Rodrigues; Raposo, Juliana D A; Freitas-de-Sousa, Luciana A; Dos-Santos, Maria Cristina; de Oliveira, Ricardo Bezerra; Veras Mourão, Rosa Helena

    2016-05-13

    Ethnobotanical studies have shown that Plathymenia reticulata Benth. (Fabaceae) has been widely used in cases of snake envenomation, particularly in Northern Brazil. In light of this, the aim of this study was to evaluate the inhibitory potential of the condensed-tannin-rich fraction obtained from the bark of P. reticulata against the main biological activities induced by Bothrops atrox venom (BaV). The chemical composition of the aqueous extract of P. reticulata (AEPr) was first investigated by thin-layer chromatography (TLC) and the extract was then fractionated by column chromatography on Sephadex LH-20. This yielded five main fractions (Pr1, Pr2, Pr3, Pr4 and Pr5), which were analyzed by colorimetry to determine their concentrations of total phenolics, total tannins and condensed tannins and to assess their potential for blocking the phospholipase activity of BaV. The Pr5 fraction was defined as the fraction rich in condensed tannins (CTPr), and its inhibitory potential against the activities of the venom was evaluated. CTPr was evaluated in different in vivo and in vitro experimental protocols. The in vivo protocols consisted of (1) pre-incubation (venom:CTPr, w/w), (2) pre-treatment (orally administered) and (3) post-treatment (orally administered) to evaluate the effect on the hemorrhagic and edematogenic activities of BaV; in the in vitro protocol the effect on phospholipase and coagulant activity using pre-incubation in both tests was evaluated. There was statistically significant inhibition (psource of natural inhibitors of the components of snake venom responsible for inducing local inflammation. Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.

  9. EFEITOS DO EXTRATO AQUOSO DA HYPTIS PECTINATA SOBRE A REGENERAÇÃO HEPÁTICA APÓS HEPATECTOMIA PARCIAL DE 70%: RESULTADOS PRELIMINARES EFFECTS OF THE AQUEOUS EXTRACT OF HYPTIS PECTINATA ON LIVER REGENERATION AFTER 70% PARTIAL HEPATECTOMY: PRELIMINAR RESULTS

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    G. B. de Melo

    2001-01-01

    Full Text Available O uso de plantas medicinais tem aumentado bastante na população mundial. O objetivo desta pesquisa é avaliar os efeitos do extrato aquoso das folhas da Hyptis pectinata, popularmente conhecida como "sambacaitá" ou "canudinho", sobre a regeneração hepática após hepatectomia parcial de 70%. Foram utilizados 24 ratos, divididos em 4 grupos: grupo OS, em que se realizou operação simulada e aplicação oral de água destilada por 4 dias; grupo OSD200, também submetido à laparotomia com manipulação do fígado e aplicação de 200 mg de extrato/Kg de animal durante o mesmo período; grupo HP, hepatectomizado a 70% após 4 dias de aplicação por via oral de água destilada; e grupo HPD200, hepatectomizado a 70% após 4 dias de administração de 200 mg extrato/Kg de animal. Foram dosadas fosfatase alcalina, bilirrubina total e as aminotransferases e estudou-se o estado III da função mitocondrial. O grupo OSD200, quando comparado ao OS, apresentou redução significativa da fosfatase alcalina. O grupo HPD200, em comparação ao HP, teve redução estatisticamente significativa no nível da AST e do estado III da função mitocondrial.The use of medicinal plants has strongly increased by the world population. The objetive of this study is to assess the effects of the aqueous extract of Hyptis pectinata leaves, popularly known as "sambacaitá" or "canudinho", on liver regeneration after 70% partial hepatectomy. Twenty four rats were divided into 4 groups: group OS, submitted to sham operation and oral administration of distilled water during 4 days; group OSD200, also submitted to sham operation and ingestion of 200 mg of extract/Kg of animal for the same period of time; group HP, which underwent 70% hepatectomy after 4 days of distilled water administration; and group HPD200, which underwent 70% hepatectomy after 4 days of oral administration of 200 mg of extract/Kg of animal. Alkaline phosphatase, total bilirubin and the serum level of

  10. Número cromossômico, análise meiótica e estimativa da viabilidade polínica em populações de Hyptis mutabilis (Rich. Briq. Number of chromosomes, microsporogenesis and pollen viability in populations of Hyptis mutabilis (Rich. Briq.

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    J.M. Fachinetto

    2009-01-01

    Full Text Available Hyptis mutabilis é uma espécie medicinal da família Lamiaceae que possui importante valor fitoterápico. Não há registros sobre estudos cromossômicos e comportamento meiótico nessa espécie em populações na região sul do Brasil. Cromossomos mitóticos, comportamento meiótico e viabilidade polínica foram estudados em populações de H. mutabilis do estado do Rio Grande do Sul, Brasil, usando coloração com orceína acética 2%. As populações apresentaram número cromossômico 2n = 28, sendo em algumas células encontrado 2n = 26 e 32 cromossomos, indicando a ocorrência de polissomatia. Ocorreram irregularidades no pareamento com associações univalentes, trivalentes, e tetravalentes, bem como presença de retardatários durante a disjunção. No entanto, os valores do índice meiótico e estimativa da viabilidade polínica foram altos, 71.19 a 92.34% e 83.25 a 96%, respectivamente.Hyptis mutabilis is a medicinal species from the Lamiaceae family, with an important phytotherapeutic value. There are no reports neither on chromosomal studies nor meiotic behavior from the Brazilian South with regard to this plant. Mitotic chromosomes, meiotic behavior, and pollen viability were studied in 05 populations of H. mutabilis from Rio Grande do Sul State, Brazil, using staining with acetic orcein 2%. The populations presented chromosome number 2n = 28, being that in some cells was found 2n = 26 and 32 chromosomes, indicating occurrence of polissomaty. Occurred irregularities on univalent, trivalent, and tetravalent pairing associations, as well as presence of lagging during the disjunction. However, the mitotic index and pollen viability estimative values were high, 71.19 to 92.34% and 83.25 to 96%, respectively.

  11. Sedative and anesthetic activities of the essential oils of Hyptis mutabilis (Rich. Briq. and their isolated components in silver catfish (Rhamdia quelen

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    L.L. Silva

    2013-09-01

    Full Text Available This study evaluated the sedative and anesthetic effects of the essential oils (EO of Hyptis mutabilis (Rich. Briq. and their isolated components on silver catfish (Rhamdia quelen. Quantitative chemical differences between the EOs obtained from leaves and inflorescences were verified, and a new chemotype rich in globulol was described. Although there were no significant differences in the time of induction for sedation and anesthesia between the EOs, only the leaf EO at 344 mg/L anesthetized all fish without side effects. Fractionation of the leaf EO was carried out by column chromatography. The isolated compounds [(+-1-terpinen-4-ol and (--globulol] showed different activity from that detected for the leaf EO in proportional concentrations and similar sedation to a eugenol control at 10 mg/L. However, fish exposed to 1-terpinen-4-ol (3 and 10 mg/L did not remain sedated for 30 min. Anesthesia was obtained with 83-190 mg/L globulol, but animals showed loss of mucus during induction and mortality at these concentrations. Synergism of the depressor effects was detected with the association of globulol and benzodiazepine (BDZ, compared with either drug alone. Fish exposed to BDZ or globulol+BDZ association showed faster recovery from anesthesia in water containing flumazenil, but the same did not occur with globulol. In conclusion, the use of globulol in aquaculture procedures should be considered only at sedative concentrations of 10 and 20 mg/L, and its mechanism of action seems not to involve the GABAA-BDZ system.

  12. THE ESSENTIAL OIL OF HYPTIS MUTABILIS IN ICHTHYOPHTHIRIUS MULTIFILIIS INFECTION AND ITS EFFECT ON HEMATOLOGICAL, BIOCHEMICAL AND IMMUNOLOGICAL PARAMETERS IN SILVER CATFISH, RHAMDIA QUELEN.

    Science.gov (United States)

    Cunha, Jessyka Arruda da; Sutili, Fernando Jonas; Oliveira, Anita Motta; Gressler, Leticia; Scheeren, Cecilia de Ávila; Silva, Lenise Lima; Vaucher, Rodrigo; Baldisserotto, Bernardo; Heinzmann, Berta

    2017-07-21

    This study evaluated the activity of leaf essential oil (EO) from Hyptis mutabilis as well as its major constituent, (-)-globulol, in infections by the parasite Ichthyophthirius multifiliis (ich). Effects on hematological, biochemical and immunological parameters of silver catfish, Rhamdia quelen, exposed to same samples also were evaluated. In the first experiment, naturally infected fish were treated with EO (0, 10 and 20 mgL-1) and ethanol, using several methods of exposure. Fish mortality and the number of trophonts per fish were assessed after 48 and 96 hr. Hour-long daily baths resulted in optimal survival, so this methodology was used for the second experiment, in which infected animals were exposed to (-)-globulol at 2.5 and 5 mgL-1. The most effective concentrations in Experiments 1 and 2 were chosen for Experiment 3, in which healthy animals were subjected to hour-long daily baths with EO (20 mgL-1) or (-)-globulol (2.5 mg L-1). Additionally, an in vitro experiment was performed with EO and globulol at the same concentrations of the in vivo test. EO and (-)-globulol increased the survival of fish infected with ich and altered certain hematological and biochemical parameters. After four days, levels of hematocrit, erythrocytes and leukocytes increased significantly in healthy animals exposed to EO. Exposure to (-)-globulol increased leukocyte number alone. No significant differences in nonspecific immunological parameters were detected when treated groups were compared to controls, but the leukocytosis observed in EO- and globulol-treated healthy animals indicates that EO and (-)-globulol increased innate immunity in these fish.

  13. Hepatoprotective and cytoprotective properties of Hyptis suaveolens against oxidative stress-induced damage by CCl(4) and H(2)O(2).

    Science.gov (United States)

    Ghaffari, Hadi; Ghassam, Behrouz Jalali; Prakash, H S

    2012-11-01

    To investigate capacity of Hyptis suaveolens (H. suaveolens) methanol extract as an antioxidant to protect against carbon tetrachloride (CCl(4))-induced oxidative stress, hepatotoxicity in Albino Wistar rats and cytoprotective effect of hydrogen peroxide (H(2)O(2)) induced cell death in HepG(2) cell line. Two different doses of methanol extract of H. suaveolens were evaluated for the hepatoprotective activity against carbon tetrachloride (CCl(4)) induced hepatotoxicity in rats. Animals in Group I: served as control, group II: H. suaveolens (100 mL/kg b.w), group III: H. suaveolens (50 mL/kg b.w) + CCl(4) (1 mg/kg), group IV: H. suaveolens (100 mL/kg b.w) + CCl(4) (1 mL/kg) and group V: CCl(4) (1 mL/kg). Histopathologic changes of liver were also evaluated. Cytotoxicity was also determined by 3, (4,5-dimethylthiazol-2-yl)2,5-diphenyl-tetrazolium bromide (MTT) assay. Oral sigle dose treatment of CCl(4) produced a marked elevation in the serum levels of aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP) and Lactate dehydrogenase (LDH). Histopathological analysis of the liver of CCl(4)-induced rats revealed marked liver cell necrosis with inflammatory collections that were conformed to increase in the levels of SOD, GSH, GST, GR and LPO. Treatment with H(2)O(2) significantly induced death of HepG(2) cell. Pretreatment with H. suaveolens methanol extract inhibited or attenuated H(2)O(2) induced cytotoxicity. This study shows that H. suaveolens methanol extract can be proposed to protect the liver against CCl(4)-induced oxidative damage in rats and protect the cells against H(2)O(2)-induced oxidative damage in HepG(2) cells. The hepatoprotective and cytoprotective effects might be correlated with its antioxidant and free radical scavenger effects. Copyright © 2012 Hainan Medical College. Published by Elsevier B.V. All rights reserved.

  14. Parâmetros bioquímicos foliares das espécies Licania tomentosa (Benth. e Bauhinia forficata (Link. para avaliação da qualidade do ar Foliar analyses of biochemical parameters of Licania tomentosa (Benth. and Bauhinia forficata (Link. species for air quality assessment

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    Otávio Luiz Gusso Maioli

    2008-01-01

    Full Text Available Foliar analysis of biochemical parameters were carried out in order to investigate the influence of air pollutants on two tropical tree species (Licania tomentosa (Benth. and Bauhinia forfícata (Link.. Special attention was given to tropospheric ozone due to the fact that concentration levels in the region were found to be up to 140 µg m-3 for a 4 h average time, which is well above the value that can cause injuries to orchides and tobacco (59 µg m-3. Other pollutants such as nitrogen and sulphur oxides were measured and their ambient concentrations were also associated to biochemical alterations in the investigated species.

  15. Liver morphology and morphometry and plasma biochemical parameters of Wistar rats that received leaf infusion of Rudgea viburnoides Benth. (Rubiaceae

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    Juliana Castro Monteiro

    2009-04-01

    Full Text Available Rudgea viburnoides leaves are widely used in popular Brazilian medicine as a diuretic, antirheumatic, hypotensive and blood depurative tea. The present study was carried out to investigate the effects of this infusion on the liver and on the plasma biochemical parameters of Wistar rats. Two groups received the R. viburnoides leaf infusion at a daily dose of 10 or 20g dry-leaves/L water, during 40 days. The histopathological analysis did not show degenerated areas or infiltration of leucocytes. Hepatic morphometry showed accumulation of fat in the hepatocytes of the treated groups. There was no significant change in the plasma levels of urea, creatinin, uric acid, direct bilirubin, cholesterol, total proteins, albumin, gamma glutamyl tranferase (gamma-GT, alanine transaminase (ALT, aspartate transaminase (AST, chlorine, phosphate and calcium. A significant reduction in the plasma levels of triacylglycerol (TAG occurred in the group that received the higher dose.As folhas de Rudgea viburnoides Benth. são utilizadas na medicina popular como diuréticas, hipotensoras, anti-reumáticas, depurativas do sangue e em regimes de emagrecimento. O presente estudo foi delineado para avaliar o efeito da infusão das folhas de R. viburnoides nos parâmetros bioquímicos plasmáticos e na morfologia e morfometria hepática de ratos Wistar adultos. Dois grupos receberam a infusão das folhas, diariamente, nas dosagens de 10 e 20 g de folhas secas/L de água, durante 40 dias. O grupo controle recebeu a mesma quantidade de água. As análises histopatológicas não mostraram áreas degeneradas e infiltrados inflamatórios. A morfometria hepática mostrou acúmulo significativo de gordura nos hepatócitos dos animais tratados, principalmente no grupo que recebeu a maior dose da infusão (8,75% de gotículas lipídicas, comparado com 0,25% delas encontradas nos animais controles. Não foram observadas alterações nos níveis plasmáticos de uréia, creatinina,

  16. Phytotoxic Effects of Nepeta meyeri Benth. Extracts and Essential Oil on Seed Germinations and Seedling Growths of Four Weed Species

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    Saban Kordali

    2015-05-01

    Full Text Available Essential oil isolated from the aerial parts of Nepeta meyeri Benth. by hydrodistilation was analysed by GC and GC-MS methods. A total 18 components were identified in the oil representing 100.0% of the oil. Main components were 4aα,7α,7aβ-nepetalactone (80.3%, 4aα,7α,7aα–nepetalactone (10.3%, trans-pulegol (3.1%, 1, 8-cineole (3.0% and β-bourbonene (2.0%. In addition, n-hexane extract of N. meyeri was analysed by using GC and GC-MS methods and 18 components were identified. Likewise, nepetalactones, 4aα,7α,7aβ-nepetalactone (83.7%, 4aα,7α,7aα–nepetalactone (3.6%, 1, 8-cineole (1.9% and α-terpinene (1.5% were the predominat compounds in the hexane extract. Three concentrations (0.5, 1.0 and 2.0 mg/mL of the essential oil and n-hexane, chloroform, acetone and methanol extracts isolated from the aerial partsand roots were tested for the herbicidal effects on the germination of the seeds of four weed species including Amaranthus retroflexus L., Chenopodium album L., Cirsium arvense L. and Sinapsis arvensis L. The essential oil of N. meyeri completely inhibited the germination of all weed seeds whereas the extracts showed various inhibition effects on the germination of the weed species. Herbicidal effect was increased with the increasing application concentrations of the extracts. In general, the acetone extract was found to be more effective as compared to the other extracts. All extracts also exhibited various inhibition effects on the seedling growths of the weed species. All extracts also tested for their phytotoxic effects on the weeds at greenhouse condition and the results showed that the oil and extracts caused mortality with 22.00-66.00% 48h after the treatments. These findings suggest that the essential oil and the extracts of N. meyeri have potentials for use as herbicides against those weed species.

  17. Color, phenolics, and antioxidant activity of blackberry (Rubus glaucus Benth.), blueberry (Vaccinium floribundum Kunth.), and apple wines from Ecuador.

    Science.gov (United States)

    Ortiz, Jacqueline; Marín-Arroyo, María-Remedios; Noriega-Domínguez, María-José; Navarro, Montserrat; Arozarena, Iñigo

    2013-07-01

    Seventy wines were produced in Ecuador under different processing conditions with local fruits: Andean blackberries (Rubus glaucus Benth.) and blueberries (Vaccinium floribundum Kunth.) and Golden Reinette apples. Wines were evaluated for antioxidant activity (AA) using the radical scavenging capacity (DPPH) method, total phenolic content (TPC) using the Folin-Ciocalteu method, total monomeric anthocyanins (TMAs) using the pH differential test, and color parameters using VIS-spectrophotometry. For blackberry wines, ellagitannins and anthocyanins were also analyzed using high-performance liquid chromatography with diode-array detection (HPLC-DAD). Apples wines (n = 40) had the lowest TPC (608 ± 86 mg/L) and AA (2.1 ± 0.3 mM Trolox). Blueberry wines (n = 12) had high TPC (1086 ± 194 mg/L) and moderate AA (5.4 ± 0.8 mM) but very low TMA (8 ± 3 mg/L), with a color evolved toward yellow and blue shades. Blackberry wines (n = 10) had the highest TPC (1265 ± 91 mg/L) and AA (12 ± 1 mM). Ellagitannins were the major phenolics (1172 ± 115 mg/L) and correlated well with AA (r = 0.88). Within anthocyanins (TMA 73 ± 16 mg/L), cyanidin-3-rutinoside (62%) and cyanidin-3-glucoside (15%) were predominant. Wines obtained by cofermentation of apples and blackberries (n = 8) showed intermediate characteristics (TPC 999 ± 83 mg/L, AA 6.2 ± 0.7 mM, TMA 35 ± 22 mg/L) between the blackberry and blueberry wines. The results suggest that the Andean berries, particularly R. glaucus, are suitable raw materials to produce wines with an in vitro antioxidant capacity that is comparable to red grape wines. © 2013 Institute of Food Technologists®

  18. Emergence and initial development of Mimosa caesalpiniifolia Benth. seedlings for different substrates = Emergência e desenvolvimento inicial de plântulas de Mimosa caesalpiniifolia Benth. em função de diferentes substratos

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    Rômulo Magno Oliveira de Freitas

    2012-04-01

    Full Text Available The present study assessed the effects of different substrates on the emergence and initial development of Mimosa caesalpiniifolia Benth seedlings. For this purpose an experiment was done in a greenhouse of the Department of Plant Sciences of Universidade Federal Rural do Semi-Árido (UFERSA, Mossoró-RN. The statistical design was completely randomized (CRD, where treatments were composed of nine substrates (T1: vermiculite, T2: coconut fiber, T3: trade hortimix®, T4: vermiculite, coconut fiber and compost (1: 1:1, T5: coconut fiber and compost (1:1, T6: coconut fiber and compost (1:2,T7: vermiculite and compost (1:1, T8, vermiculite and compost organic (1:2 and T9: organic compost. All treatments were represented by four replications containing 25 seeds. The following variables were evaluated: emergency percentage, emergence rate index, length of shoot and root, stem diameter, dry leaves, stem, root and total leaf area, specific leaf area, leaf area ratio, and leaf weight ratio. Data were submitted to the Tukey test at 5% probability. The coconut fiber substrates, commercial hortimix® and vermiculite proved superior promoted good emergence and early development of seedlings. The mixture of vermiculite, coconut fiber and compost (1:1:1, coconut fiber and compost (1:1, coconut fiber and compost (1:2 and organic compost and vermiculite (1: 1 was not shown to be adequate for the cultivation of seedlings. There was no emergency in the pure organic compound in the mixture of compost and vermiculite (1:2.Key words - Vermiculite. Organic compost. Coconut fiber. Sabiá. Substrates. = Objetivou-se com o presente trabalho avaliar o efeito de diferentes substratos na emergência e desenvolvimento inicial de plântulas de sabiá (Mimosa caesalpiniifolia Benth.. Para isso, foi instalado um experimento em casa de vegetação do Departamento de Ciências Vegetais da Universidade Federal Rural do Semi-árido (UFERSA, Mossoró-RN. O delineamento estat

  19. Avaliação do potencial antioxidante do Croton cajucara benth e seus efeitos sobre o estresse oxidativo no diabetes mellitus experimental

    OpenAIRE

    Éder Marcolin

    2008-01-01

    O Diabetes Mellitus (DM) é uma doença endócrino-metabólica freqüente, com expectativa de alcançar 350 milhões de pessoas no mundo em 2025, segundo a Organização Mundial da Saúde (OMS). Estudos experimentais e clínicos sugerem que o estresse oxidativo esteja envolvido na patogênese e na progressão desta doença. A espécie Croton cajucara BENTH (CcB) é uma planta da região amazônica que tem suas folhas e casca do caule utilizadas pela população na forma de chá ou cápsulas, para tratar várias doe...

  20. The Main Chemical Composition and in vitro Antifungal Activity of the Essential Oils of Ocimum basilicum Linn. var. pilosum (Willd. Benth

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    Ji-Wen Zhang

    2009-01-01

    Full Text Available The essential oils of the aerial parts of Ocimum basilicum Linn.var. pilosum (Willd. Benth., an endemic medicinal plant growing in China, was obtained by hydrodistillation and analysed by GC-MS. Fifteen compounds, representing 74.19% of the total oil were identified. The main components were as follows: linalool (29.68%, (Z-cinnamic acid methyl ester (21.49%, cyclohexene (4.41%, α- cadinol (3.99%, 2,4-diisopropenyl-1-methyl-1-vinylcyclohexane (2.27%, 3,5-pyridine-dicarboxylic acid, 2,6-dimethyl-diethyl ester (2.01%, β-cubebene (1.97%, guaia-1(10,11-diene (1.58%, cadinene (1.41% (E-cinnamic acid methyl ester (1.36% and β-guaiene (1.30%. The essential oils showed significant antifungal activity against some plant pathogenic fungi.

  1. Coleus barbatus Benth and Ocimum basilicum L. (Lamiaceae, New Host Plants to Spodoptera cosmioides (Walker (Lepidoptera: Noctuidae in Sinop, State of Mato Grosso, Brazil

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    Evaldo Pires

    2014-04-01

    Resumo. Coleus barbatus Benth e Ocimum basilicum L. são espécies de plantas comumente utilizadas com fins medicinais e gastronômicos, respectivamente. Lagartas do gênero Spodoptera são generalistas devido à ampla variedade de plantas que utilizam como recurso alimentar. O objetivo desta pesquisa foi registrar a ocorrência de Spodoptera cosmioides (Walker (Lepidoptera: Noctuidae no município de Sinop, Mato Grosso, Brasil, e ainda, relatar C. barbatus e O. basilicum como potenciais plantas hospedeiras para esta espécie de inseto. Recomenda-se ainda a inclusão de S. cosmioides em monitoramentos visando o Manejo Integrado de Pragas (MIP nestas plantas.

  2. Chemical Composition, and Antibacterial (against Staphylococcus aureus and Free-Radical-Scavenging Activities of the Essential Oil of Scrophularia amplexicaulis Benth.

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    Ardalan Pasdaran

    2012-07-01

    Full Text Available Chemical composition of the essential oil obtained from the aerial parts of Scrophularia amplexicaulis Benth. was analyzed, for the first time, by the gas chromatography/mass spectrometry (GC-MS and gas chromatography/flame ionization detection (GC-FID. A total yield of 3 mg of essential oil per100 g of plant dry mass was obtained, and 27 compounds were identified, representing 97. 7 % of total oil. The essential oil were characterized by a high content of oxygenated monoterpenes and phenolic derivatives. The main constituents were eugenol (53.8%, eugenol acetate (24.5%, b -caryophyllene (5.7%, caryophyllene oxide (6.4% and aromadendrene oxide II (2.1%. The antimicrobial activity of the essential oil was tested against Staphylococcus aureus using the well diffusion method, and t he free-radical-scavenging activity was assessed by the 2,2-diphenyl-picryl-hydrazyl (DPPH assay.

  3. Preparative isolation and purification of coumarins from Angelica dahurica (Fisch. ex Hoffn) Benth, et Hook. f (Chinese traditional medicinal herb) by high-speed counter-current chromatography.

    Science.gov (United States)

    Liu, Renmin; Li, Aifeng; Sun, Ailing

    2004-10-15

    A preparative high-speed counter-current chromatography (HSCCC) method for isolation and purification of coumarins from Angelica dahurica (Fisch. ex Hoffin) Benth, et Hook. f(Baizhi in Chinese) was successfully established by using n-hexane-methanol-water as the two-phase solvent system in gradient elution mode. The upper phase of n-hexane-methanol-water (5:5:5, v/v) was used as the stationary phase of HSCCC. The mobile phase used in HSCCC was the lower phase of n-hexane-methanol-water (5:5:5, v/v) and n-hexane-methanol-water (5:7:3, v/v) that was changed in gradient. Three major components including imperatorin, isoimperatorin and oxypeucedanine were isolated, each at over 98% purity as determined by high-performance liquid chromatography (HPLC). The peak fractions of HSCCC were identified by 1H-NMR and 13C-NMR.

  4. Verificação da atividade antibacteriana de sabonete líquido contendo extrato glicólico de Dimorphandra mollis Benth.

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    KETYLIN FERNANDA MIGLIATO

    2009-12-01

    Full Text Available Dimorphandra mollis Benth., Compositae, falsobarbatimão, é utilizada topicamente como cicatrizante, adstringente e antimicrobiano. No presente estudo, verificou-se a atividade antibacteriana de sabonete líquido contendo extrato glicólico de D. mollis (EGD em diferentes concentrações (8, 15 e 20% e em diferentes pHs (6 e 8. Foram preparadas cinco formulações (F de sabonete: F1 - triclosan (0,1%, F2 - EGD (8%, F3 - EGD (15%, F4 - EGD (20% e F5 - sem conservante. Cascas de D. mollis foram secas em estufa de ar circulante e pulverizadas. Os extratos brutos foram preparados por turbo-extração utilizando-se etanol. Após filtração, os extratos foram concentrados em evaporador rotatório, liofilizados e ressuspendidos em propilenoglicol para a obtenção do extrato glicólico. A atividade antibacteriana foi verificada pelo método de difusão em ágar, empregando cilindros em placa. Placas contendo Staphylococcus aureus, Pseudomonas aeruginosa e Escherichia coli foram incubadas a 37ºC durante 24 horas. Após incubação, as leituras foram realizadas com paquímetro, observando-se o diâmetro do halo de inibição de crescimento bacteriano. Verificouse que o sabonete líquido contendo triclosan provocou inibição do crescimento bacteriano em ambos os pHs; já os sabonetes sem conservante e contendo EGD, independente da concentração e do pH empregados, não apresentaram atividade antibacteriana. Palavras-chave: Atividade antibacteriana. Sabonete líquido. Dimorphandra mollis Benth.

  5. Composição química dos óleos essenciais de Hyptis suaveolens (L.) Poit.: uma espécie de ocorrência natural no nordeste paraense.

    OpenAIRE

    Zoghbi, Maria das Graças Bichara; Jardim, Mário Augusto Gonçalves; Oliveira, Jorge; Trigo, José R.

    2008-01-01

    Os óleos essenciais de dez espécimes de Hyptis suaveolens coletados nos municípios de Santar ém Novo, Magalhães Barata e Bragança (PA), foram obtidos por hidrodestilação e analisados por CG/ DIC e CG/EM. As amostras coletadas em Santarém Novo forneceram óleos ricos em endo-fenchol/1,8- cineol (parte aérea: 30,5%/16,5%; planta inteira: 28,6%/25,8%), e 1,8-cineol (parte aérea: 28,5%, planta inteira: 17,1% - 24,3%; folha: 31,4%). Em Bragança foram encontrados óleos ricos em 1,8-cineo...

  6. Two myrmecophilous scale insects, Cryptostigma urichi (Cockerell (Hemiptera, Coccidae and Farinococcus multispinosus Morrison (Hemiptera, Pseudococcidae, cohabiting inside branches of Anadenanthera falcata (Benth. Speg. (Fabales, Fabaceae in the Cerrado area of São Paulo State, Brazil

    Directory of Open Access Journals (Sweden)

    André R. Nascimento

    2012-12-01

    Full Text Available The soft scale Cryptostigma urichi (Cockerell, 1894 and the mealybug Farinococcus multispinosus Morrison, 1922 are reported cohabiting inside branches of Anadenanthera falcata (Benth. Speg. (Angico-preto, both tended by the ant Azteca oecocordia Longino, 2007 (Hymenoptera, Formicidae. This interaction was observed in the Cerrado region sensu stricto, at the Federal University of São Carlos, UFSCar, Brazil. A. oecocordia is recorded for the first time in Brazil.

  7. The germination of bush mint (Hyptis marrubioides EPL. seeds as a function of harvest stage, light, temperature and duration of storage=Germinação de sementes de hortelã-do-campo (Hyptis marrubioides EPL. em função da época de colheita, da luz, temperatura e armazenamento.

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    Ricardo Monteiro Corrêa

    2011-10-01

    Full Text Available This study evaluated the effect of light, temperature, physiologic stage at harvest and length of time on the germination of Hyptis marrubioides seeds. Two trials were conducted. The first experiment was performed immediately after the seed harvest and consisted of a 2 x 3 x 3 factorial design with 2 environmental conditions (light and dark, 3 temperatures (20, 30 and 20/30ºC and 3 seed harvest times (green colored seeds, light brown seeds and dark brown seeds. The second experiment was conducted in the presence of light at a temperature of 30ºC and consisted of a 4 x 3 factorial design, with 4 storage times (0, 6, 12 and 18 months and the 3 three harvest physiologic stages used in the previous experiment. Both of the experiments were conducted in randomized blocks, with 4 replications of 100 seeds. Light did not affect germination. By contrast, a temperature of 20ºC retarded the germination process, although the percentage of germinating seeds was not affected. Seed storage and the different harvest physiologic stages affected the Speed of germination index (SGI and the germination percentage. Seeds that were harvested at the more mature stage (dark brown color could be stored for up to 18 months.O presente trabalho teve como objetivos avaliar a influência da luz, da temperatura, do estádio fisiológico na colheita e do armazenamento na germinação das sementes de Hyptis marrubioides. Foram realizados dois experimentos, sendo o primeiro implantado logo após a colheita das sementes, constituído por um fatorial 2 x 3 x 3, com 2 ambientes (luz e escuro x 3 temperaturas (20, 30 e 20/30ºC x 3 épocas de colheita das sementes (sementes com coloração verde, coloração marrom claro e coloração marrom escuro. O segundo experimento, em presença de luz e à temperatura de 30ºC, foi constituído por um esquema fatorial 4 x 3, sendo 4 tempos de armazenamento (0, 6, 12 e 18 meses e 3 três estádios fisiológicos de coleta mencionados no

  8. Two myrmecophilous scale insects, Cryptostigma urichi (Cockerell (Hemiptera, Coccidae and Farinococcus multispinosus Morrison (Hemiptera, Pseudococcidae, cohabiting inside branches of Anadenanthera falcata (Benth. Speg. (Fabales, Fabaceae in the Cerrado area of São Paulo State, Brazil Duas cochonilhas mirmecófilas, Cryptostigma urichi (Cockerell (Hemiptera, Coccidae e Farinococcus multispinosus Morrison (Hemiptera, Pseudococcidae, coabitando no interior de ramos de Anadenanthera falcata (Benth. Speg. (Fabales, Fabaceae em área de Cerrado do Estado de São Paulo, Brasil

    Directory of Open Access Journals (Sweden)

    André R. Nascimento

    2012-12-01

    Full Text Available The soft scale Cryptostigma urichi (Cockerell, 1894 and the mealybug Farinococcus multispinosus Morrison, 1922 are reported cohabiting inside branches of Anadenanthera falcata (Benth. Speg. (Angico-preto, both tended by the ant Azteca oecocordia Longino, 2007 (Hymenoptera, Formicidae. This interaction was observed in the Cerrado region sensu stricto, at the Federal University of São Carlos, UFSCar, Brazil. A. oecocordia is recorded for the first time in Brazil.O coccídeo Cryptostigma urichi (Cockerell, 1894 e o pseudococcídeo Farinococcus multispinosus Morrison, 1922 são registrados coabitando no interior de galhos de Anadenanthera falcata (Benth. Speg. (Angico-preto, ambos atendidos pela formiga Azteca oecocordia Longino, 2007 (Hymenoptera, Formicidae. Essa interação foi observada em uma região de Cerrado sensu stricto, na Universidade Federal de São Carlos, UFSCar, Brasil. A. oecocordia é registrada pela primeira vez para o Brasil.

  9. Abordagem fitoquímica, composição bromatológica e atividade antibacteriana de Mimosa tenuiflora (Wild Poiret E Piptadenia stipulacea (Benth Ducke =Phytochemical approach, bromatologic composition and antibacterial activity of Mimosa tenuiflora (Wild Poiret and Piptadenia stipulacea (Benth Ducke

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    Denise Aline Casimiro Bezerra

    2011-01-01

    Full Text Available A necessidade de encontrar novas drogas eficazes no combate microbiano tem aumentado a cada dia e estimulado a busca de novos compostos naturais com atividades biológicas. Neste trabalho, realizaram-se estudo fitoquímico e análises microbiológicas com os extratos etanólicos das espécies (jurema-preta Mimosa tenuiflora (Wild Poiret e (juremabranca Piptadenia stipulacea (Benth Ducke, frente a linhagens de bactérias patogênicas. O pó da casca do caule de ambas as espécies foi submetido à avaliação bromatológica e determinados os teores de Matéria Seca, Matéria Mineral, Proteína Bruta, Fibra em Detergente Neutro e Energia Bruta. Os resultados para a prospecção química indicaram a presença de taninos e outros compostos fenólicos, bem como a presença de saponinas em ambos os extratos. Os extratos das duas espécies demonstraram que mais de uma parte das plantas possui atividade antimicrobiana. A composição bromatológica da casca do caule de jurema-preta e jurema-branca apresentou teores diferenciados para as variáveis avaliadas.The need to find new efficient drugs to combat microbes has increasedthe search for new natural compounds with biological activities. In this work, phytochemical studies and microbiological analysis were carried out with the ethanol extracts of Mimosa tenuiflora (Wild Poiret and Piptadenia stipulacea (Benth Ducke on pathogenic bacteria strains. The bark powders of both species were submitted to bromatologic evaluation and the levels of dry matter, mineral matter, crude protein, neutral detergent fiber (NDF and crude energy were determined. The results of the chemical search chemical showed the presence of tannins and other phenolic compounds as well as the presence of saponins in both extracts. The microbiologic evaluation of the extracts of both species showed that more than one part of the plants had antimicrobial activity. The bromatologic composition of the bark powder of Mimosa tenuiflora (Wild

  10. Pogostemon cablin (Blanco) Benth

    African Journals Online (AJOL)

    SAM

    2014-06-04

    Jun 4, 2014 ... based on the zones of inhibition and MIC values were higher indicating that leaves have a potential ... Nature has bestowed on us a very rich botanical ... Patchouli (P. cablin) is a herb belonging to the Labiatae family originating from Southeast Asia. Patchouli leaves contain an essential oil which is made ...

  11. Avaliação da atividade antioxidante e antimicrobiana dos extratos e frações orgânicas de Mimosa caesalpiniifolia Benth. (Mimosaceae

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    Marcelo José Dias Silva

    2012-04-01

    Full Text Available A espécie Mimosa caesalpiniifolia Benth. (Mimosaceae, conhecida popularmente como sabiá e cerva viva, é uma planta arbórea encontrada na caatinga nordestina brasileira, amplamente utilizada pela população na forma de infusões para o tratamento de feridas, bronquites e anti-inflamatório. Diante do exposto, os objetivos deste estudo, foram determinar as atividades antioxidantes e antimicrobianas do extrato etanólico das folhas (EHM, caules (EHL, cascas do caule (EHC, raízes (EHR e frações obtidas das folhas de M. caesalpiniifolia Benth. A atividade antioxidante foi avaliada através do método de captação do radical DPPH, enquanto a atividade antimicrobiana foi avaliada pelo método de microdiluição em caldo, sobre leveduras, bactérias Gram-positivas e Gram-negativas. A capacidade antioxidante mostrou que a fração acetato de etila (Fr-EtOAc foi diretamente proporcional ao teor de polifenóis totais com IC50 de 20,08 ± 0,10 µg/ mL e 721,29±0,60 mg de EAG (equivalentes de ácido gálico por g de extrato. Na atividade antimicrobiana, todos os extratos e frações exibiram atividade inibitória de crescimento frente aos micro-organismos microrganismos avaliados e em concentrações variando de 5 a 1000 μg/mL. A Fr-EtOAc apresentou valores promissores de inibição de crescimento frente a fungos, como Candida glabrata (ATCC 90030 e Candida krusei (ATCC 6258, com concentrações de 20 e 40 μg/mL, respectivamente. Estes resultados são importantes, pois são os primeiros a serem realizados com a espécie M. caesalpiniifolia.

  12. Synthesis of amide derivatives of 6{alpha},7{beta}-di-hydroxyvouacapan-17{beta}-oic acid isolated from the Pterodon polygalaeflorus Benth fruits (leguminosae); Sintese de amidas derivadas do acido 6{alpha},7{beta}-di-hidroxivouacapan-17{beta}-oico isolado dos frutos de Pterodon polygalaeflorus Benth (leguminosae)

    Energy Technology Data Exchange (ETDEWEB)

    Teixeira, A.L.; Belinelo, V.J.; Stefani, G.M.; Pilo-Veloso, D. [Minas Gerais Univ., Belo Horizonte, MG (Brazil). Dept. de Quimica]. E-mail: dorila@dedalus.lcc.ufmg.br; Reis, G.T. [Universidade de Itauna, MG (Brazil). Dept. de Ciencias; Ferreira-Alves, D.L. [Minas Gerais Univ., Belo Horizonte, MG (Brazil). Dept. de Farmacologia

    2001-04-01

    Hydro-alcoholic infusions from fruits of Pterodon polygalaeflorus Benth, commonly known as 'Sucupira branca', are used in Brazilian folk medicine for rheumatic problems and throat infections. Since it has been verified that furanediterpene 6?,7?-di-hydroxyvouacapan-17?-oic acid (ADV), isolated from the hexane extract of these fruits presents anti-inflammatory, analgesic, and plant growth regulatory activity, a variety of ADV derivatives has been synthesized with the aim of obtaining more information about the structure-activity relationships of this series of compounds. In this work four new amide (4-7) derivatives of ADV have been synthesized. Their structures were established by spectroscopic data, including 2D-NMR methods. (author)

  13. Inhibitory Effect of the Hexane Fraction of the Ethanolic Extract of the Fruits of Pterodon pubescens Benth in Acute and Chronic Inflammation

    Directory of Open Access Journals (Sweden)

    Jaqueline Hoscheid

    2013-01-01

    Full Text Available Fruits of Pterodon pubescens Benth have been used traditionally for the treatment of rheumatism, sore throat, and respiratory disorders, and also as anti-inflammatory, analgesic, depurative, tonic, and hypoglycemic agent. The study was aimed at evaluating the anti-inflammatory activity of the hexane fraction of an ethanolic extract of P. pubescens fruits. The oil from P. pubescens fruits was extracted with ethanol and partitioned with hexane. The anti-inflammatory activity was measured with increasing doses of the hexane fraction (FHPp by using a carrageenan-induced rat model of pleurisy and a rat model of complete Freund's adjuvant-induced arthritis by using an FHPp dose of 250 mg/kg for 21 days. Treatment with an FHPp resulted in anti-inflammatory activity in both models. The results of biochemical, hematological, and histological analyses indicated a significant decrease in glucose, cholesterol, and triglycerides levels (18.32%, 34.20%, and 41.70%, resp. and reduction in the numbers of total leukocytes and mononuclear cells. The FHPp dose of 1000 mg/kg induced no changes in behavioral parameters, and no animal died. The results of this study extend the findings of previous reports that have shown that administration of extracts and fractions obtained from species of the genus Pterodon exhibits anti-inflammatory activity and lacks toxicity.

  14. The extraction of essential oil from patchouli leaves (Pogostemon cablin Benth) using microwave hydrodistillation and solvent-free microwave extraction methods

    Science.gov (United States)

    Y Putri, D. K.; Kusuma, H. S.; E Syahputra, M.; Parasandi, D.; Mahfud, M.

    2017-12-01

    Patchouli plant (Pogostemon cablin Benth) is one of the important essential oil-producing plant, contributes more than 50% of total exports of Indonesia’s essential oil. However, the extraction of patchouli oil that has been done in Indonesia is generally still used conventional methods that require enormous amount of energy, high solvent usage, and long time of extraction. Therefore, in this study, patchouli oil extraction was carried out by using microwave hydrodistillation and solvent-free microwave extraction methods. Based on this research, it is known that the extraction of patchouli oil using microwave hydrodistillation method with longer extraction time (240 min) only produced patchouli oil’s yield 1.2 times greater than solvent-free microwave extraction method which require faster extraction time (120 min). Otherwise the analysis of electric consumption and the environmental impact, the solvent-free microwave extraction method showed a smaller amount when compared with microwave hydrodistillation method. It is conclude that the use of solvent-free microwave extraction method for patchouli oil extraction is suitably method as a new green technique.

  15. Induction of Osmoregulation and Modulation of Salt Stress in Acacia gerrardii Benth. by Arbuscular Mycorrhizal Fungi and Bacillus subtilis (BERA 71

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    Abeer Hashem

    2016-01-01

    Full Text Available The role of soil microbiota in plant stress management, though speculated a lot, is still far from being completely understood. We conducted a greenhouse experiment to examine synergistic impact of plant growth promoting rhizobacterium, Bacillus subtilis (BERA 71, and arbuscular mycorrhizal fungi (AMF (Claroideoglomus etunicatum; Rhizophagus intraradices; and Funneliformis mosseae to induce acquired systemic resistance in Talh tree (Acacia gerrardii Benth. against adverse impact of salt stress. Compared to the control, the BERA 71 treatment significantly enhanced root colonization intensity by AMF, in both presence and absence of salt. We also found positive synergistic interaction between B. subtilis and AMF vis-a-vis improvement in the nutritional value in terms of increase in total lipids, phenols, and fiber content. The AMF and BERA 71 inoculated plants showed increased content of osmoprotectants such as glycine, betaine, and proline, though lipid peroxidation was reduced probably as a mechanism of salt tolerance. Furthermore, the application of bioinoculants to Talh tree turned out to be potentially beneficial in ameliorating the deleterious impact of salinity on plant metabolism, probably by modulating the osmoregulatory system (glycine betaine, proline, and phenols and antioxidant enzymes system (SOD, CAT, POD, GR, APX, DHAR, MDAHR, and GSNOR.

  16. ALTERAÇÕES NA ARQUITETURA TÍPICA DE Tipuana tipu (Benth. O. Kuntze NA ARBORIZAÇÃO DE RUAS DE CURITIBA, PARANÁ

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    Rogério Bobrowski

    2013-01-01

    Full Text Available The urban structure and equipments can restrict the correct development of trees and its typical architecture. Tipuana tipu (Benth. O. Kuntze (rosewood has been pruned continuously for being a species of large size and for being the most planted tree in the city of Curitiba. Because of that, this work aimed to assess the changes in the typical architecture of Tipuana tipu on street trees of Curitiba through completely randomized design containing a check plot composed by 6 trees and a treatment with 6 trees under utility lines and another with 6 trees in streets without utility lines. From horizontal photographs of trees there were obtained dendrometric variables based on a metric scale attached at DBH. Thus, the morphometric indexes were calculated to describe dimensional relationships for the species on trees without pruning (check plot and on pruned trees (treatments. The results showed that raising pruning change typical architecture of rosewood modifying its outline and its form, in addition to harm its aesthetic effect. The indexes that can express changes significantly (p<0.05, were: range index, salience index, crown angle, crown proportion and relation RH/PH.

  17. Antibacterial activity of 14, 15-dihydroajugapitin and 8-o-acetylharpagide isolated from Ajuga bracteosa Wall ex. Benth against human pathogenic bacteria.

    Science.gov (United States)

    Ganaie, Hilal A; Ali, Md Niamat; Ganai, Bashir A; Meraj, Maryum; Ahmad, Mudasar

    2017-02-01

    Ajuga bracteosa Wall ex. Benth. (Lamiaceae) commonly known as Bungle Weed has been in use since ancient times and is mentioned Ayurvedic literature. The upper ground parts of the plant are used for treatment of various diseases. The weed is credited with astringent, febrifugal, stimulant, aperient, tonic, diuretic and depurative properties and is used for the treatment of gout and rheumatism, palsy and amenorrhoea. Two compounds 1) 14, 15-dihydroajugapitin and 2) 8-o-acetylharpagide were isolated from the aerial parts of the plant and tested for antibacterial activity against various pathogenic bacteria by agar well diffusion method. Compound 1 and 2 showed maximum antibacterial activity against Escherichia coli with zone of inhibitions of 25. 0 ± 1.4 mm and 22.6 ± 0.9 mm respectively. The MIC value of compound 1 and 2 ranged between 500 and 1000 μg/ml. It could be concluded that both compounds isolated from the aerial parts of Ajuga bracteosa possess antibacterial activity against pathogens. Copyright © 2016 Elsevier Ltd. All rights reserved.

  18. Primeros ensayos para el cultivo y caracterización del aceite esencial de Conobea scoparioides (Cham. & Schltdl. Benth. para el Pacífico colombiano

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    Robert Tulio González Mina

    2010-01-01

    Full Text Available Conobea scoparioides (Cham. & Schltdl. Benth. es una planta silvestre de humedales de la que no hay antecedentes de su cultivo o domesticación. El presente trabajo intenta obtener resultados preliminares para su reproducción en condiciones experimentales de siembras simulando su entorno natural contrastada con siembras en materas. Como se ha reportado previamente interés por esta especie como fuente de aceites esenciales ricos en timol, sustancia valiosa de uso industrial y medicinal, se analiza la composición cromatográfica de los volátiles para una población local. Se enuncia un nuevo quimiotipo para el aceite esencial de C. scoparioides procedente de los humedales del Pacífico colombiano. Se presentan los resultados de los ensayos sobre la reproducción vegetativa y por semillas de la especie en cuestión, aunque los resultados reproductivos indican un limitado potencial agrícola para esta especie.

  19. Controles físico, físico-químico, químico e microbiológico dos frutos de Dimorphandra mollis Benth., Fabaceae

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    Silviane Z. Hubinger

    Full Text Available Considerando-se a qualidade dos fitoterápicos, é importante salientar que a preocupação com esta questão inclui rigoroso acompanhamento das diferentes etapas de desenvolvimento e produção, desde a coleta do vegetal até o produto final. O controle de qualidade de drogas vegetais e seus extratos é essencial quando utilizados como matéria-prima para o desenvolvimento de fitoterápicos. Neste trabalho foram utilizadas diversas técnicas (farmacopeicas e não farmacopeicas visando estabelecer parâmetros de qualidade dos frutos de Dimorphandra mollis Benth. (Leguminosae, popularmente conhecida como faveiro. Os resultados obtidos determinaram as características físico-químicas da droga vegetal e mostraram que o pó dos frutos e o extrato etanólico 70% de D. mollis apresentam um teor de flavonóides de 10,25% e 17,21%, respectivamente. A análise fitoquímica preliminar dos frutos caracterizou a presença de taninos, saponinas e flavonóides. Na análise microbiológica do extrato não houve crescimento de patógenos entre os testes realizados.

  20. Distinct substrate specificities and unusual substrate flexibilities of two hydroxycinnamoyltransferases, rosmarinic acid synthase and hydroxycinnamoyl-CoA:shikimate hydroxycinnamoyl-transferase, from Coleus blumei Benth.

    Science.gov (United States)

    Sander, Marion; Petersen, Maike

    2011-06-01

    cDNAs and genes encoding a hydroxycinnamoyl-CoA:hydroxyphenyllactate hydroxycinnamoyltransferase (CbRAS; rosmarinic acid synthase) and a hydroxycinnamoyl-CoA:shikimate hydroxycinnamoyltransferase (CbHST) were isolated from Coleus blumei Benth. (syn. Solenostemon scutellarioides (L.) Codd; Lamiaceae). The proteins were expressed in E. coli and the substrate specificity of both enzymes was tested. CbRAS accepted several CoA-activated phenylpropenoic acids as donor substrates and D-(hydroxy)phenyllactates as acceptors resulting in ester formation while shikimate and quinate were not accepted. Unexpectedly, amino acids (D-phenylalanine, D-tyrosine, D-DOPA) also yielded products, showing that RAS can putatively catalyze amide formation. CbHST was able to transfer cinnamic, 4-coumaric, caffeic, ferulic as well as sinapic acid from CoA to shikimate but not to quinate or acceptor substrates utilized by CbRAS. In addition, 3-hydroxyanthranilate, 3-hydroxybenzoate and 2,3-dihydroxybenzoate were used as acceptor substrates. The reaction product with 3-aminobenzoate putatively is an amide. For both enzymes, structural requirements for donor and acceptor substrates were deduced. The acceptance of unusual acceptor substrates by CbRAS and CbHST resulted in the formation of novel compounds. The rather relaxed substrate as well as reaction specificity of both hydroxycinnamoyltransferases opens up possibilities for the evolution of novel enzymes forming novel secondary metabolites in plants and for the in vitro formation of new compounds with putatively interesting biological activities.

  1. Phloem loading in Coleus blumei in the absence of carrier-mediated uptake of export sugar from the apoplast. [Coleus blumei Benth

    Energy Technology Data Exchange (ETDEWEB)

    Turgeon, R.; Gowan, E. (Cornell Univ., Ithaca, NY (USA))

    1990-11-01

    Phloem loading in Coleus blumei Benth. leaves cannot be explained by carrier-mediated transport of export sugar from the apoplast into the sieve element-companion cell complex, the mechanism by which sucrose is thought to load in other species that have been studied in detail. Uptake profiles of the export sugars sucrose, raffinose, and stachyose into leaf discs were composed of two components, one saturable and other other not. Saturable (carrier-mediated) uptake of all three sugars was almost completely eliminated by the inhibitor p-chloromercuribenzenesulfonic acid (PCMBS). However, when PCMBS was introduced by transpiration into mature leaves it did not prevent accumulation of {sup 14}C-photosynthate in minor veins or translocation of labeled photosynthate from green to nonchlorophyllous regions of the leaf following exposure to {sup 14}CO{sub 2}. The efficacy of introducing inhibitor solutions in the transpiration stream was proven by observing saffranin O and calcofluor white movement in the minor veins and leaf apoplast. PCMBS introduced by transpiration completely inhibited phloem loading in tobacco leaves. Phloem loading in C. blumei was also studied in plasmolysis experiments. The carbohydrate content of leaves was lowered by keeping plants in the dark and then increased by exposing them to light. The solute level of intermediary cells increased in the light (phloem loading) in both PCMBS-treated and control tissues. A mechanism of symplastic phloem loading is proposed for species that translocate the raffinose series of oligosaccharides.

  2. Influence of Npk inorganic fertilizer treatment on the proximate composition of the leaves of Ocimum gratissimum (L.) and Gongronema latifolium (benth).

    Science.gov (United States)

    Osuagwu, G G E; Edeoga, H O

    2013-04-15

    The influence of NPK inorganic fertilizer treatment on the proximate composition of the leaves of Ocimum gratissimum (L.) and Gongronema latifolium (Benth) was investigated. Cultivated O. gratissimum and G. latifolium were treated with NPK (15:15:15) fertilizer at 100, 200, 300, 400 and 500 kg h(-1) treatment levels in planting buckets derived using the furrow slice method two months after seedling emergence. No fertilizer treatment served as control. The leaves of the plants were harvested for analysis one month after treatment. The leaf was used for the analysis because it the most eaten part. Fertilizer treatment significantly (p leaves of both plants. On the other hand, fertilizer treatment significantly, (p leaves of the plants. The increase in the concentrations of these substances as a result of fertilizer of fertilizer treatment might be due to the role of fertilizer in chlorophyll content of plant's leaves, which in turn enhanced the process of photosynthesis leading to increased synthesis of these substances. The decrease in the carbohydrate content might be due to its conversion to other materials in the plants. The results obtained were discussed in line with current literatures.

  3. CONTENIDO DE TANINOS EN LA CORTEZA DE DOS ESPECIES DE PARÁCATA (Erythroxylon compactum Rose y Senna skinneri Benth. Irwin & Barneby

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    Serafín Colín-Urieta

    2013-01-01

    Full Text Available Se obtuvieron taninos de la corteza de Parácata (Erythroxylon compactum Rose y Senna skinneri Benth. Irwin & Barneby mediante extracción acuosa aplicando un diseño experimental 2k, con k = 4 y n = 2. Los factores y niveles fueron: Factor A = tamaño de partícula (0.417, 6.68 mm, Factor B = tiempo de extracción (120, 180 min, Factor C = temperatura de extracción (80, 87 °C, Factor D = relación sólido-líquido (1:15, 1:12. Se evaluó la calidad curtiente de los taninos. Se curtió una piel de venado con taninos de S. skinneri y se determinó la resistencia al desagarre y la resistencia a la tensión. Los resultados de las propiedades físico-mecánicas de la piel curtida fueron superiores a los valores mínimos recomendados por las normas mexicanas. Con base en lo anterior, se estima que la corteza de S. skinneri pudiera ser susceptible de aprovechamiento para la extracción de taninos y así usarlos en el curtido de pieles.

  4. A Comprehensive Review on the Phytochemical Constituents and Pharmacological Activities of Pogostemon cablin Benth.: An Aromatic Medicinal Plant of Industrial Importance

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    Mallappa Kumara Swamy

    2015-05-01

    Full Text Available Pogostemon cablin Benth. (patchouli is an important herb which possesses many therapeutic properties and is widely used in the fragrance industries. In traditional medicinal practices, it is used to treat colds, headaches, fever, nausea, vomiting, diarrhea, abdominal pain, insect and snake bites. In aromatherapy, patchouli oil is used to relieve depression, stress, calm nerves, control appetite and to improve sexual interest. Till now more than 140 compounds, including terpenoids, phytosterols, flavonoids, organic acids, lignins, alkaloids, glycosides, alcohols, aldehydes have been isolated and identified from patchouli. The main phytochemical compounds are patchouli alcohol, α-patchoulene, β-patchoulene, α-bulnesene, seychellene, norpatchoulenol, pogostone, eugenol and pogostol. Modern studies have revealed several biological activities such as antioxidant, analgesic, anti-inflammatory, antiplatelet, antithrombotic, aphrodisiac, antidepressant, antimutagenic, antiemetic, fibrinolytic and cytotoxic activities. However, some of the traditional uses need to be verified and may require standardizing and authenticating the bioactivity of purified compounds through scientific methods. The aim of the present review is to provide comprehensive knowledge on the phytochemistry and pharmacological activities of essential oil and different plant extracts of patchouli based on the available scientific literature. This information will provide a potential guide in exploring the use of main active compounds of patchouli in various medical fields.

  5. Primeros ensayos para el cultivo y caracterización del aceite esencial de Conobea scoparioides (Cham. & Schltdl. Benth. para el Pacífico colombiano

    Directory of Open Access Journals (Sweden)

    Robert Tulio González Mina

    2011-01-01

    Full Text Available Conobea scoparioides (Cham. & Schltdl. Benth. es una planta silvestre de humedales de la que no hay antecedentes de su cultivo o domesticación. El presente trabajo intenta obtener resultados preliminares para su reproducción en condiciones experimentales de siembras simulando su entorno natural contrastada con siembras en materas. Como se ha reportado previamente interés por esta especie como fuente de aceites esenciales ricos en timol, sustancia valiosa de uso industrial y medicinal, se analiza la composición cromatográfica de los volátiles para una población local. Se enuncia un nuevo quimiotipo para el aceite esencial de C. scoparioides procedente de los humedales del Pacífico colombiano. Se presentan los resultados de los ensayos sobre la reproducción vegetativa y por semillas de la especie en cuestión, aunque los resultados reproductivos indican un limitado potencial agrícola para esta especie.

  6. Reproductive phenology and sharing of floral resource among hummingbirds (Trochilidae in inflorescences of Dahlstedtia pinnata (Benth. Malme. (Fabaceae in the Atlantic forest

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    CAIO C.C. MISSAGIA

    2014-12-01

    Full Text Available The purpose of this study was to investigate the reproductive phenology and sharing of floral resource (nectar of Dahlstedtia pinnata (Benth. Malme. (Fabaceae, endemic of Atlantic forest, among hummingbirds. For the phenology, we looked at the presence of reproductive structures in the plants, and for floral resource sharing, the frequency of potential pollinators and foraging behaviors were examined. This study was conducted in Pedra Branca State Park, in state of Rio de Janeiro, in a dense ombrophilous forest, between August 2010 and August 2011. Flowering occurred between December 2010 and March 2011, and fruiting between April and June 2011. Hummingbirds' foraging schedules differed significantly, with legitimate visits to the flowers occurring in the morning and illegitimate visits occurring during late morning and the afternoon. Five species visited flowers, three of which were legitimate visitors: Phaethornis ruber, P. pretrei, and Ramphodon naevius. Amazilia fimbriata and Thalurania glaucopis females only visited illegitimately. Phaethornis ruber robbed nectar (78% of illegitimate visits, n=337. Ramphodon naevius, with a territorial foraging behavior and a body size bigger than that of other observed hummingbird species, dominated the floral visits, which suggests that D. pinnata is an important nourishing resource for this endemic bird of the Atlantic forest, currently globally categorized as Near Threatened.

  7. Induction of Osmoregulation and Modulation of Salt Stress in Acacia gerrardii Benth. by Arbuscular Mycorrhizal Fungi and Bacillus subtilis (BERA 71).

    Science.gov (United States)

    Hashem, Abeer; Abd Allah, E F; Alqarawi, A A; Al-Huqail, A A; Shah, M A

    2016-01-01

    The role of soil microbiota in plant stress management, though speculated a lot, is still far from being completely understood. We conducted a greenhouse experiment to examine synergistic impact of plant growth promoting rhizobacterium, Bacillus subtilis (BERA 71), and arbuscular mycorrhizal fungi (AMF) (Claroideoglomus etunicatum; Rhizophagus intraradices; and Funneliformis mosseae) to induce acquired systemic resistance in Talh tree (Acacia gerrardii Benth.) against adverse impact of salt stress. Compared to the control, the BERA 71 treatment significantly enhanced root colonization intensity by AMF, in both presence and absence of salt. We also found positive synergistic interaction between B. subtilis and AMF vis-a-vis improvement in the nutritional value in terms of increase in total lipids, phenols, and fiber content. The AMF and BERA 71 inoculated plants showed increased content of osmoprotectants such as glycine, betaine, and proline, though lipid peroxidation was reduced probably as a mechanism of salt tolerance. Furthermore, the application of bioinoculants to Talh tree turned out to be potentially beneficial in ameliorating the deleterious impact of salinity on plant metabolism, probably by modulating the osmoregulatory system (glycine betaine, proline, and phenols) and antioxidant enzymes system (SOD, CAT, POD, GR, APX, DHAR, MDAHR, and GSNOR).

  8. First report of toxicity of Xylopiaparviflora (A. Rich.) Benth (Annonaceae) root bark's essential oil against cowpea seed bruchid, Callososbruchus maculatus Fabricius (Coleoptera: Chrysomelidae: Bruchinae).

    Science.gov (United States)

    Babarinde, Samuel Adelani; Pitan, Olufemi Olutoyin Richard; Olatunde, Ganiyu Olatunji; Ajala, Michael Oluwole

    2015-01-01

    The fumigant toxicity of Xylopia parviflora (A. Rich.) Benth (Annonaceae) root bark's essential oil (EO) against cowpea seed bruchid, Callosobruchus maculatus, was investigated in the laboratory. Dose had significant (P < 0.0001) effect on mortality at 6 hours after treatment (HAT) at a concentration of 6.25 μL/mL air which exerted 81.70% mortality, while there was no mortality in all other lower doses. At 12 HAT, 75.05% and 90.00% mortality were observed at doses of 3.15 and 6.25 μL/mL air, respectively. It was significantly (P < 0.05) higher than the mortality (50.58%) observed when 0.78 μL/mL air was applied. The lethal time for 50% of assayed adults (LT50) obtained when the bruchid was exposed to X. parviflora EO at a dose of 6.25 μL/mL air (2.71 h) was significantly lower than LT50 obtained at exposure of bruchid to other lower doses of 0.78-3.15 μL/mL air.

  9. Growth, production and chemical composition of the essential oil in hortelã-do-campo (Hyptis marrubioides Epl. in function of the irradition level / Influência do nível de irradiância no crescimento, produção e composição química do óleo essencial de hortelã-do-campo (Hyptis marrubioides Epl.

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    Priscila Pereira Botrel

    2009-07-01

    Full Text Available In the present work, the level of irradiation of 20, 60 and 100% of natural light on the growth, content and composition of the essnetial oil of “hortelã do campo” (Hyptis marrubioides was evaluated. The experiment was conducted in the completely randomized design with twenty-five replicates per treatment. After 132 days of cultivation, plant growth and the content and compositoin of the essntial oil were investigated. At 100% of irradiation, the plants presented less height as compared with the shaded treatments. The ratio branch/leaf was higher at the level of irradiation of 20%. It was found that in the full sunshine, the plants showed lower height as compared with the shaded treatments. The leaf/ branch ratio was higher at the lowest level of irradition of 20%. The number of branches and the plant mass accumulation were greatest at the level of irradition of 100%. The content of essential oil was not influenced by the levels of irradiation, but the yield was greater when grown at the level of 100% of irradiation. The chemical composition of the essential oil of Hyptis marrubioides did not present any alterations with the different levels of irradiation, except for iso-3-tujanol and ?-cadineno, which presented highest concentrations in plants grown, respectively at 100 and 60% of irradiation. The major component of teh essential oil of H. marrubioides, independent of the levels of irradiation was cistujona oxygenated monoterpene (37.78%, followed by sequiterpenes hiydrocarbonetes (E-cariofileno (14.93%, ?-copaeno (11.03% and ?-muuroleno (9.60%.No presente trabalho avaliou-se o nível de irradiância de 20, 60 e 100% de luz natural no crescimento, teor e a composição do óleo essencial de hortelã-do-campo (Hyptis marrubioides. O experimento foi conduzido no delineamento inteiramente casualizado, com vinte e cinco repetições por tratamento. Após 132 dias de cultivo analisou-se o crescimento da planta, o teor e a composição do

  10. Influencia da intensidade luminosa e do substrato no crescimento, no conteudo de clorofila e na fotossintese de Cabralea canjerana (Vell.) Mart. Subsp. canjerana, Calophyllum brasiliense Camb. e centrolobium robustum (Vell.) Mart. Ex Benth., na fase ju

    OpenAIRE

    Carvalho, Paulo Ernani Ramalho, 1946-

    2013-01-01

    Visando estudar a auto-ecologia de três espécies florestais brasileiras; Cabralea canjerana (Vell.) Mart. subsp. canjerana (canjarana); Callophyllum brasiliense Camb. (guanandi) e Centrolobium robustum (VelI.) Mart. ex Benth. (araribá-rosa), estudou-se o comportamento na fase juvenil de mudas envasadas em dois substratos (Colombo e Santa Helena) e sob intensidades luminosas de 10%, 30%, 50% e 100%. Para todas as espécies, foram estudados vários parâmetros ecofisiológicos: altura, diâmetro do ...

  11. Dynamics of litter production and decomposition of araribá (Centrolobium tomentosum Guill. ex Benth. _ Fabaceae) in a riparian forest, Jacaré-Pepira river, São Paulo

    OpenAIRE

    Aidar, Marcos P.M.; Joly, Carlos A.

    2003-01-01

    This study was conducted in a remaining riparian forest (42 ha) in the Jacaré-Pepira river, where Centrolobium tomentosum Guill. ex Benth., an early secondary and deciduous species, is the most important tree in the seasonal semideciduous forest. C. tomentosum's litter production reached 4.2 kg.ind-1, with two peaks of leaf shedding (January/February _ 25.1% and July/August _ 52.3%) representing an input to soil surface of (g.ind.¹ year¹): 74.7 N; 6.1 P; 29.0 K; 73.9 Ca; 25.8 Mg and 14.1 S. L...

  12. A planta facilitadora Trischidium molle (Benth.) H. E. Ireland (Leguminosae) e sua relação com a comunidade de plantas em ambiente semi-árido no Nordeste do Brasil

    OpenAIRE

    Meiado, Marcos Vinícius

    2008-01-01

    A interação entre os arbustos de Trischidium molle (Benth.) H. E. Ireland (Leguminosae) e a comunidade de plantas em ambiente semi-árido foi avaliada para responder as seguintes questões: (1) Os fatores abióticos diferem sob a copa dos arbustos quando comparado com os locais abertos? (2) A disponibilidade de recurso é maior sob a copa dos arbustos do que nos locais abertos? (3) Os arbustos de T. molle promovem a facilitação intra-específica em ambientes semi-áridos? (4) Os efei...

  13. EFECTO DE LA REDUCCIÓN DE NITRÓGENO EN EL METABOLISMO DE COMPUESTOS FENÓLICOS EN BROTES DE Castilleja tenuiflora Benth. CULTIVADOS EN BIORREACTOR DE INMERSIÓN TEMPORAL

    OpenAIRE

    Medina Pérez, Virginia

    2015-01-01

    Castilleja tenuiflora Benth. (“hierba del cáncer”) es recomendada por la medicina tradicional Mexicana para tratar síntomas asociados con el cáncer, que podrían deberse a que acumula compuestos fenólicos como flavonoides y feniletanoides. El nitrógeno (N) es un elemento fundamental para el crecimiento y desarrollo de las plantas y, su deficiencia provoca el incremento en la concentración de compuestos fenólicos y de la actividad de enzimas claves de su biosíntesis como es la fenilalanina amon...

  14. Elicitación con oligosacáridos de Fusarium oxysporum f. sp. lycopersici en la biosíntesis de feniletanoides de cultivos in vitro de Castilleja tenuiflora Benth.

    OpenAIRE

    Cardenas Sandoval, Blanca Alejandra

    2013-01-01

    Castilleja tenuiflora Benth. (Orobanchaceae) es una planta silvestre, conocida como “hierba del cáncer” y utilizada de manera tradicional para tratar enfermedades con sintomatología cancerosa. Esta especie sintetiza feniletanoides, que son de gran interés farmacológico por sus actividades biológicas como antioxidante, anti cancerígena, regulación del sistema inmune, neuro y hepato protectora. Por ello la importancia de conocer su ruta de biosíntesis la cual ha sido estudia en l...

  15. Investigating the potential of metal-organic framework material as an adsorbent for matrix solid-phase dispersion extraction of pesticides during analysis of dehydrated Hyptis pectinata medicinal plant by GC/MS.

    Science.gov (United States)

    Aquino, Adriano; Ferreira, Jordana Alves; Navickiene, Sandro; Wanderley, Kaline A; de Sá, Gilberto F; Júnior, Severino A

    2012-01-01

    Metal-organic frameworks aluminum terephthalate MIL-53 and Cu-benzene-1,3,5-tricarboxylate (BTC) were tested for extraction of pyrimethanil, ametryn, dichlofluanid, tetraconazole, flumetralin, kresoximmethyl, and tebuconazole from the medicinal plant Hyptis pectinata, with analysis using GC/MS in the selected ion monitoring mode. Experiments carried out at different fortification levels (0.1, 0.5, and 1.0 microg/g) resulted in recoveries in the range 61 to 107% with RSD values between 3 and 12% for the metal-organic framework materials. Detection and quantification limits ranged from 0.02 to 0.07 and 0.05 to 0.1 microg/g, respectively, for the different pesticides studied. The method developed was linear over the range tested (0.04-20.0 microg/g), with correlation coefficients ranging from 0.9987 to 0.9998. Comparison of MIL-53 and Cu-BTC with C18-bonded silica showed good performance of the MIL-53 metal-organic framework as a sorbent for the pesticides tested.

  16. Effects of Maytenus ilicifolia Mart. and Bauhinia candicans Benth infusions on onion root-tip and rat bone-marrow cells

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    Marjori Leiva Camparoto

    2002-01-01

    Full Text Available Medicinal plants are widely used to treat various diseases, and in Brazil the plants Maytenus ilicifolia Mart. and Bauhinia candicans Benth are commonly used in popular medicine. However, there are a large number of compounds in plants which can produce alterations in genetic material, and this study was conducted to investigate any possible mutagenic and cytotoxic effects that M. ilicifolia and B. candicans infusions may have on the cell cycle and chromosomes. Infusions were prepared with in natura leaves to give two concentrations of infusions, one at the concentration normally used by the population in general and the other at 10 times this value (i.e. 3.5 and 35 mg/mL for M. ilicifolia and 0.465 and 4.65 mg/mL for B. candicans. Onion (Allium cepa L. root-tip cells (RTC and Wistar rat bone-marrow cells (BMC were used as test systems in in vivo assays. The M. ilicifolia infusions at both concentrations, and the B. candicans infusion at the lower concentration, had no statistically significant depressive mitotic effect on RTC. A statistically significant depressive mitotic effect on RTC was found with the more concentrated (4.65 mg/mL B. candicans infusion as compared with a negative control. In BMC, infusions of B. candicans and M. ilicifolia produced no statistically significant increase in the number of chromosome alterations or rates of cell division as compared to controls. The significance of these findings are discussed in the light of the use of these plants as therapeutic agents.

  17. Primary, Secondary Metabolites, Photosynthetic Capacity and Antioxidant Activity of the Malaysian Herb Kacip Fatimah (Labisia Pumila Benth Exposed to Potassium Fertilization under Greenhouse Conditions

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    Ali Ghasemzadeh

    2012-11-01

    Full Text Available A randomized complete block design was used to characterize the relationship between production of total phenolics, flavonoids, ascorbic acid, carbohydrate content, leaf gas exchange, phenylalanine ammonia-lyase (PAL, soluble protein, invertase and antioxidant enzyme activities (ascorbate peroxidase (APX, catalase (CAT and superoxide dismutase (SOD in Labisia pumila Benth var. alata under four levels of potassium fertilization experiments (0, 90, 180 and 270 kg K/ha conducted for 12 weeks. It was found that the production of total phenolics, flavonoids, ascorbic acid and carbohydrate content was affected by the interaction between potassium fertilization and plant parts. As the potassium fertilization levels increased from 0 to 270 kg K/ha, the production of soluble protein and PAL activity increased steadily. At the highest potassium fertilization (270 kg K/ha L. pumila exhibited significantly higher net photosynthesis (A, stomatal conductance (gs, intercellular CO2 (Ci, apparent quantum yield (ɸ and lower dark respiration rates (Rd, compared to the other treatments. It was found that the production of total phenolics, flavonoids and ascorbic acid are also higher under 270 kg K/ha compared to 180, 90 and 0 kg K/ha. Furthermore, from the present study, the invertase activity was also found to be higher in 270 kg K/ha treatment. The antioxidant enzyme activities (APX, CAT and SOD were lower under high potassium fertilization (270 kg K/ha and have a significant negative correlation with total phenolics and flavonoid production. From this study, it was observed that the up-regulation of leaf gas exchange and downregulation of APX, CAT and SOD activities under high supplementation of potassium fertilizer enhanced the carbohydrate content that simultaneously increased the production of L. pumila secondary metabolites, thus increasing the health promoting effects of this plant.

  18. A phenylalanine ammonia-lyase ortholog (PkPAL1) from Picrorhiza kurrooa Royle ex. Benth: molecular cloning, promoter analysis and response to biotic and abiotic elicitors.

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    Bhat, Wajid Waheed; Razdan, Sumeer; Rana, Satiander; Dhar, Niha; Wani, Tariq Ahmad; Qazi, Parvaiz; Vishwakarma, Ram; Lattoo, Surrinder K

    2014-09-01

    Picrorhiza kurrooa Royle ex Benth. is a highly reputed medicinal herb utilised in the preparation of a number of herbal drug formulations, principally due to the presence of novel monoterpene iridoid glycosides kenned as picrosides. Phenylalanine ammonia-lyase catalyses an important rate-limiting step in phenylpropanoid pathway and supplies precursors like cinnamic acid, vanillic acid, ferulic acid, etc., to a variety of secondary metabolites including picrosides. The imperilled status of P. kurrooa coupled with lack of information regarding biogenesis of picrosides necessitates deciphering the biosynthetic pathway for picrosides. In the present study, a PAL gene, designated PkPAL1 was isolated from P. kurrooa. The cDNA is 2312 bp in length, consisting of an ORF of 2142 bp encoding for a 713 amino acid protein having a predicted molecular weight of 77.66 kDa and an isoelectric point of pH 6.82. qRT-PCR analysis of various tissues of P. kurrooa showed that PkPAL1 transcript levels were highest in the leaves, consistent with picroside accumulation pattern. Using Genome walking, a 718 bp promoter region was also isolated resulting in identification of distinct cis-regulatory elements including TGA-element, TGACG-motif, CGTCA-motif, etc. qRT-PCR indicated up-regulation of PkPAL1 by methyl jasmonate, salicylic acid, 2,4-dicholorophenoxy acetic acid and UV-B elicitations that corroborated positively with the identified cis-elements within the promoter region. Moreover, altitude was found to have a positive effect on the PkPAL1 transcript levels, driving the expression of PkPAL1 abundantly. Based on docking analysis, we identified eight residues as potentially essential for substrate binding in PkPAL1. Copyright © 2014 Elsevier B.V. All rights reserved.

  19. Anti-nociceptive Activity of Ethnomedicinally Important Analgesic Plant Isodon rugosus Wall. ex Benth: Mechanistic Study and Identifications of Bioactive Compounds.

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    Zeb, Anwar; Ahmad, Sajjad; Ullah, Farhat; Ayaz, Muhammad; Sadiq, Abdul

    2016-01-01

    Isodon rugosus Wall. ex Benth. is extensively used as traditional medicine for the management of various types of pain including tooth ache, gastric pain, abdominal pain, ear ache, and generalized body pain. The current study is designed to scientifically verify the purported uses of I. rugosus as analgesic agent and to figure out its possible mechanism of action. Bioactive compounds responsible for analgesic activity were identified using GC and GC-MS analysis. Analgesic potentials were evaluated using acetic acid induced writhing, hot plate test, and formalin induced paw licking test. In acetic acid induced writhing chloroform fraction (Ir.Chf) exhibited 53% analgesia while formalin test displayed 61% inhibition at phase-I and 45% at phase-II respectively at a dose of 100 mg/kg. Similarly, in hot plate test Ir.Chf displayed average reaction time of 7 min at 15, 30, 45, and 60 min intervals. The possible mechanism of action was found to be the central pathway via opioidergic receptors as the mice showed morphine like analgesic activity at pre-administration of naloxone (opioid antagonist) in hot plate and formalin tests. In GC-MS analysis, 83 compounds were identified among which eight compounds including benzyl alcohol, sebacic acid, myristic acid, phytol, sugiol, Tocopherol, α-Amyrin, and stigmasterol were sorted out as previously reported analgesic compounds. Current study revealed that analgesic potential of I. rugosus can attributed to the presence of analgesic compounds. It may also be concluded that opioids receptors are involved in the analgesic mechanism of I. rugosus due to effective antagonism of nalaxone.

  20. Abscisic Acid Induced Changes in Production of Primary and Secondary Metabolites, Photosynthetic Capacity, Antioxidant Capability, Antioxidant Enzymes and Lipoxygenase Inhibitory Activity of Orthosiphon stamineus Benth.

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    Mohd Hafiz Ibrahim

    2013-07-01

    Full Text Available An experiment was conducted to investigate and distinguish the relationships in the production of total phenolics, total flavonoids, soluble sugars, H2O2, O2−, phenylalanine ammonia lyase (PAL activity, leaf gas exchange, antioxidant activity, antioxidant enzyme activity [ascorbate peroxidase (APX, catalase (CAT, superoxide dismutase (SOD and Lipoxygenase inhibitory activity (LOX] under four levels of foliar abscisic acid (ABA application (0, 2, 4, 6 µM for 15 weeks in Orthosiphon stamineus Benth. It was found that the production of plant secondary metabolites, soluble sugars, antioxidant activity, PAL activity and LOX inhibitory activity was influenced by foliar application of ABA. As the concentration of ABA was increased from 0 to 6 µM the production of total phenolics, flavonoids, sucrose, H2O2, O2−, PAL activity and LOX inhibitory activity was enhanced. It was also observed that the antioxidant capabilities (DPPH and ORAC were increased. This was followed by increases in production of antioxidant enzymes APX, CAT and SOD. Under high application rates of ABA the net photosynthesis and stomatal conductance was found to be reduced. The production of primary and secondary metabolites displayed a significant positive relationship with H2O2 (total phenolics, r2 = 0.877; total flavonoids, r2 = 0.812; p ≤ 0.05 and O2− (total phenolics, r2 = 0.778; total flavonoids, r2 = 0.912; p ≤ 0.05. This indicated that increased oxidative stress at high application rates of ABA, improved the production of phytochemicals.

  1. Germinação e vigor de plântulas de Parkia platycephala Benth. em diferentes substratos e temperaturas

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    Romário Bezerra e Silva

    Full Text Available RESUMO Conhecida popularmente como fava de bolota, a Parkia platycephala Benth. pertence a família Fabaceae, ocorre em áreas de transição Caatinga-Cerrado e apresenta grande potencial madeireiro, paisagístico e, principalmente, forrageiro. O objetivo da pesquisa é fornecer informações para a elaboração de protocolos de testes de germinação de sementes e vigor de plântulas de P. platycephala, submetidas a diferentes subtratos e temperaturas. Para avaliar o efeito do substrato e da temperatura, as sementes foram semeadas entre os substratos: vermiculita, areia, pó de coco, bagaço da cana-de-açúcar, tropstrato®, papel (RP, marca Germitest e papel mata-borrão, distribuídas em caixas plásticas transparentes, todos sob luz contínua em germinador tipo Biochemical oxygen Demand (B.O.D., com temperaturas constantes de 5; 10; 15; 20; 25; 30; 35 e 40 °C, e temperaturas alternadas de 20-30 ºC e 25-35 ºC. Foram avaliadas as variáveis germinação, índice de velocidade de germinação, tempo médio da germinação, comprimento da raiz primária e da parte aérea, e massa seca da raiz primária e da parte aérea. A temperatura alternada de 25-35 °C combinada com o substrato vermiculita é recomendada para realização de testes de germinação e vigor de P. platycephala.

  2. ESTUDO PROSPECTIVO SOBRE PROPRIEDADES ANTINEOPLÁSICAS DE PLANTAS DA FAMÍLIA FABACEAE COM ÊNFASE EM Mimosa caesalpiniifolia BENTH.

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    Jurandy Nascimento Silva

    2016-09-01

    Full Text Available A demanda por utilização de métodos alternativos para o tratamento do câncer está aumentando a cada ano e estudos sugerem ação citotóxica e antitumoral de um grande número de moléculas de origem vegetal contra diferentes tipos de cânceres. Mimosa caesalpiniifolia Benth. é uma planta nativa do bioma Caatinga, presente em grande parte da Região Nordeste do Brasil que vem sendo progressivamente cultivada do Maranhão ao Rio de Janeiro e apresenta-se como promissora na buscas de moléculas com atividades antineoplásicas. Nesse contexto, este trabalho teve como objetivo realizar uma prospecção científica e tecnológica sobre a utilização dessa espécie vegetal no tratamento de neoplasias e/ou cânceres. Esse levantamento prospectivo mostrou que de um modo geral que as patentes foram depositadas em maior número nas bases WIPO (Word Intelectual Property Organization e EPO (European Patent Office, com classificação internacional principalmente nas áreas de ciência médica ou veterinária e higiene (A61K e alimentos, produtos alimentícios ou bebidas não alcoólicas (A23L. As produções científicas estão alocadas principalmente nas áreas de medicina, farmacologia, toxicologia, farmacêutica e bioquímica, genética e biologia molecular, com um ápice de publicações em nossa década atual, demostrando o interesse da comunidade científica.

  3. Alterações fisiológicas em sementes de Tachigalia multijuga (Benth. (mamoneira relacionadas aos métodos para a superação da dormência Physiological modifications of Tachigalia multijuga (Benth. (mamoneira seeds related to dormacy overcoming methods

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    Eduardo Euclydes de Lima e Borges

    2004-06-01

    Full Text Available Este trabalho teve como objetivo estudar as alterações fisiológicas causadas por métodos de quebra da dormência em sementes de Tachigalia multijuga (Benth provenientes de três matrizes. Compararam-se os efeitos do ácido sulfúrico, da água fervente e do desponte na porcentagem de embebição, na porcentagem e velocidade de germinação, na atividade de alfagalactosidase e betamananase, na síntese de proteína e na alteração da membrana que recobre o embrião. Não houve germinação em sementes tratadas com água quente. Todos os tratamentos resultaram em porcentagem de germinação superior (PThis work aimed to study the effects of methods to overcome the dormancy of Tachigalia multijuga (Benth seeds. It was compared the effects of the sulfuric acid, of the boiling water and of the it blunts in the imbibition percentage, in the percentage and speed of germination, in the activity of alpha galactosidase and beta mananase, in the protein synthesis and in the alteration in the membrane that recovers the embryo. There was no germination in seeds treated with hot water. All the treatments resulted in germination percentage superior (P<0.05 to the control, except for the seeds of Cachoeira, where the treatment with sulfuric acid for 10 minutes was the same. On the other hand, the speed of germination of the control was only different (P<0.05 from all the treatments in seeds of Araponga 2. The water percentage of the seeds treated with hot water by 60 seconds went the same to those of the control and different (P<0.05 from the treated with hot water by 30 minutes and by acid for 20 minutes. The activities of the enzymes and proteins content during the germination were different (P<0.05 among the treatments with water and acid. It is discussed the alterations of the membrane sugars content that recovers the embryo seeds.

  4. Validated high-performance liquid chromatographic method for the standardisation of Ptychopetalum olacoides Benth., Olacaceae, commercial extracts Validação de método de cromatografia líquida de alta performance para padronização de extratos comerciais de Ptychopetalum olacoides Benth., Olacaceae

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    Renata Colombo

    2010-11-01

    Full Text Available Ptychopetalum olacoides Benth., Olacaceae, popularly known as marapuama or muirapuama or miriantã, is a species native to the Amazonian region of Brazil. Extracts of the bark of the plant have been used traditionally for its stimulating and aphrodisiac properties and currently commercialised by the herbal industry as constituents in a wide range of phytomedicines. Fractionation by open column chromatography followed by preparative HPLC-UV/PAD of the stem bark and of three commercial extracts of P. olacoides allowed the isolation of three components that were common to all extracts analysed, and these were identified by NMR to be vanillic acid, protocatechuic acid and theobromine. Vanillic acid, which has been proposed as a phytochemical marker for P. olacoides, was employed as an external standard in the development and validation of a rapid qualitative and quantitative HPLC assay for the analyte. The recoveries values of the developed method were 99.02% and the LOD and LOQ values were 0.033 and 0.11 mg.L-1, respectively. The described method may be applied to the standardisation of herbs, extracts or phytomedicines commercialised as marapuama.Ptychopetalum olacoides Benth., Olacaceae, popularmente conhecida como marapuama, muirapuama ou miriantã, é uma espécie nativa da região da Amazônia do Brasil. Extratos das cascas da planta são tradicionalmente usados por suas propriedades estimulantes e afrodisíacas, e frequentemente comercializados como constituinte de uma grande variedade de formulações fitoterápicas. O fracionamento por coluna cromatográfica aberta seguida por CLAE-UV/PAD das cascas do caule de três extratos comerciais de P. olacoides permitiram o isolamento de três substâncias comuns em todos os extratos analisados. Os compostos foram identificados por RMN como ácido vanílico, ácido protocatecuíco e teobromina. O ácido vanílico foi utilizado como marcador fitoquímico para P. olacoides e empregado como padr

  5. Mobilização de reservas durante a germinação das sementes e crescimento das plântulas de Caesalpinia peltophoroides Benth. (Leguminosae-Caesalpinoideae Mobilization of the reserves during germination of seeds and growth of seedlings of Caesalpinia peltophoroides Benth. (Leguminosae-Caesalpinoideae

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    Viviana Borges Corte

    2006-12-01

    Full Text Available Este trabalho teve como objetivo estudar a mobilização de reservas de sementes de Caesalpinia peltophoroides Benth. durante a germinação e crescimento inicial das plântulas. As variações nas reservas de carbiodratos, lipídios e proteínas foram analisadas desde o período pré-germinativo (0 a 5 dias após a semeadura - DAS até a total senescência e abscisão dos cotilédones, aos 35 DAS, por meio de testes bioquímicos nos cotilédones das sementes. Os resultados indicaram que os lipídios constituem o principal composto de reserva nos cotilédones, contribuindo com cerca de 50% de massa seca. Carboidratos solúveis representaram 32%, as proteínas solúveis 7,7% e o amido 6,8% de massa seca dos cotilédones. Os lipídios sofreram marcante decréscimo entre 5 e 10 dias após a semeadura, período em que se observou elevada taxa de crescimento das plântulas. Carboidratos e proteínas solúveis exibiram tendência gradativa de queda, enquanto no amido, isso quase não foi detectado. A redução do peso de massa seca dos cotilédones foi bem correlacionada com o aumento da biomassa da plântula.This work aimed at studying the mobilization seed reserves during germination and initial growth of seedlings of Caesalpinia peltophoroides Benth. The variations in carbohydrate, lipid and protein reserves were analyzed from the pre-sprouting period (0 to 5 days after sowing -DAS to the total senescence and abscission of seeds. The results showed that lipids constitute the main reserve compound in the cotyledon, contributing with almost 50 % of its dry mass weight. Soluble carbohydrates represent 32 %, the soluble proteins 7.7 % and starch 6.8 % of the dry mass weight of cotyledons. Lipids showed a marked decrease between 5 and 10 days after sowing, period of a high seedling growth rate. Carbohydrates and soluble proteins showed a gradual tendency to decrease, while starch was almost non-detectable. The reduction in cotyledon dry mass of weight

  6. Sobre a botânica, a etnofarmacologia e a química de Calycophyllum spruceanum (Benth. Hook. f. ex K. Schum

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    A.B. SANTOS

    2016-01-01

    Full Text Available RESUMO O fato de possuir espécies com potencial econômico ilimitado, faz com que o ambiente amazônico se torne alvo constante da biopirataria e da extração predatória dos recursos. Muitas espécies deste ambiente sequer possuem catalogação e, mesmo assim, encontram risco eminente de desaparecimento. Neste âmbito, trabalhos que reúnam dados sobre estas espécies possuem grande valor científico, cultural e econômico, sendo este o estímulo que desencadeou a produção desta revisão. Aqui a espécie Calycophyllum spruceanum (Benth. Hook. f. ex K. Schum., uma Rubiaceae amazônica, é contemplada por meio de características botânicas, indicações etnofarmacológicas e propriedades químicas. Popularmente conhecida como mulateiro, a espécie é frequentemente receitada na etnomedicina como cicatrizante e rejuvenescedor, além de ser usada no controle de manchas de pele. Como peculiaridade botânica, mulateiro apresenta tronco retilíneo com epiderme fina esverdeada que evolui para uma periderme castanho-escuro, que é anualmente renovada. Em termos químicos, há destaque para a presença de alcalóides, taninos e, sobretudo, secoiridóides (7-metoxididerrosideo, 6´-acetil-β-D-glucopiranosildiderrosideo e 8-0-tigloildiderrosideo são peculiares à espécie. Mesmo com propriedades fotoprotetoras comprovadas, C. spruceanum ainda carece de pesquisas, sobretudo àquelas voltadas para a produção ex situ da planta e àquelas que demonstrem a relação entre a ecologia da planta e a produção de metabólitos funcionais para a indústria.

  7. Demonstration of biological activities of extracts from Isodon rugosus Wall. Ex Benth: Separation and identification of bioactive phytoconstituents by GC-MS analysis in the ethyl acetate extract.

    Science.gov (United States)

    Zeb, Anwar; Ullah, Farhat; Ayaz, Muhammad; Ahmad, Sajjad; Sadiq, Abdul

    2017-05-30

    Since long, natural sources have been explored for possible managements of various diseases. In this context, the study is designed to evaluate Isodon rugosus Wall. ex Benth for biological potentials including antibacterial, anthelmintic, insecticidal, anti-termites and anti-Pharaoh activities followed by GC-MS analysis of active fraction to identify various bioactive compounds. I. rugosus was investigated against eight bacterial strains using well diffusion method and microdilution method with ceftriaxone as positive control. Similarly, the insecticidal activity was carried out against Tribolium castaneum, Rhyzopertha dominica, Monomorium pharaonis and Heterotermis indicola following contact toxicity method. Likewise, anthelmintic activity was performed against Ascaridia galli and Pherethima posthuma using albendazole as positive control, in which the paralysis and death times of the worms were observed. The GC-MS analysis of the most active solvent fraction was performed for identifications of various bioactive compounds. Among the tested samples of I. rugosus, flavonoids and ethyl acetate fraction exhibited high antibacterial activities. The crude saponins showed highest anthelmintic activity against Pherethima posthuma and Ascaridia galli with death times of 27.67 and 29.22 min respectively at concentrations of 40 mg/ml. In insecticidal activity, chloroform fraction and saponins exhibited notable results against R. dominica (60 and 70%) and T. castaneum (70 and 76%) at concentration of 200 mg/ml. In anti-termite assay, all the plant samples showed overwhelming results, i.e. all the 25 termites were killed on the 3rd day. Similarly, in anti-Pharaoh activity, the chloroform, ethyl acetate and saponins fractions were most potent, each exhibiting LD 50 of ethyl linolate, cyclohexanone, hinokione, methyl palmitate, ethyl palmitate and stigmasterol acetate. Based on our current results, it can be concluded that I. rugosus possess strong antibacterial, insecticidal

  8. In vivo anti-inflammatory and anti-ulcerogenic activities of extracts from wild growing and in vitro plants of Castilleja tenuiflora Benth. (Orobanchaceae).

    Science.gov (United States)

    Sanchez, Paul Mauricio; Villarreal, María Luisa; Herrera-Ruiz, Maribel; Zamilpa, Alejandro; Jiménez-Ferrer, Enrique; Trejo-Tapia, Gabriela

    2013-12-12

    Castilleja tenuiflora Benth. (Orobanchaceae) is a perennial shrub used since the 16(th) century in Mexican traditional medicine for the treatment of a number of health disorders including inflammation, stomach pain and tumors. The aim of the present study was to evaluate the cytotoxic, anti-inflammatory and anti-ulcerogenic activities of ethyl acetate (EaE), methanol (ME) and aqueous extracts (AE) of Castilleja tenuiflora wild grown (CtW) and in vitro plants (CtIv). Phytochemical analysis of the phenylethanoid glycoside (PhG) and iridoid glycoside (IG) components was carried out by chromatographic methods. In vitro cytotoxic activity of the extracts was evaluated in the following four carcinoma cell lines: colon (HF-6), breast (MCF-7), prostate (PC-3), and nasopharyngeal (KB). The topical anti-inflammatory activity was evaluated in mouse ear edema induced by 12-O-tetradecanoylphorbol-13-acetate (TPA). Anti-ulcerogenic activity was evaluated in rats using an absolute ethanol-induced acute gastric ulcer model. The main compounds in the extracts were isoverbascoside, verbascoside and aucubin and their concentration depended both on the solvent used and on the plant material origin. None of the extracts showed cytotoxicity against the tested cell lines. In contrast, CtWEaE, CtWAE and CtIvEaE (1.6 mg/ear) showed moderate anti-inflammatory activity similar to dexamethasone (1 mg/ear) with a 38.2, 39.3 and 49.1% decrease of inflammation, respectively. CtWEaE and CtIvEaE (100 mg/kg) showed high anti-ulcerogenic activity with 88.3 and 83.1% inhibition, respectively, compared to famotidine (20 mg/kg, 32.8% inhibition). Castilleja tenuiflora extracts provided significant gastric protection in an acute ulcer induction model and topical anti-inflammatory activity in a mouse ear edema model. These activities are related to verbascoside and may explain the traditional use of Castilleja tenuiflora in the treatment of anti-inflammatory and gastrointestinal disorders. Cultured

  9. Abordagem fitoquímica, composição bromatológica e atividade antibacteriana de Mimosa tenuiflora (Wild Poiret E Piptadenia stipulacea (Benth Ducke - doi: 10.4025/actascibiolsci.v33i1.5366 Phytochemical approach, bromatologic composition and antibacterial activity of Mimosa tenuiflora (Wild Poiret and Piptadenia stipulacea (Benth Ducke - doi: 10.4025/actascibiolsci.v33i1.5366

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    Onaldo Guedes Rodrigues

    2011-02-01

    Full Text Available A necessidade de encontrar novas drogas eficazes no combate microbiano tem aumentado a cada dia e estimulado a busca de novos compostos naturais com atividades biológicas. Neste trabalho, realizaram-se estudo fitoquímico e análises microbiológicas com os extratos etanólicos das espécies (jurema-preta Mimosa tenuiflora (Wild Poiret e (jurema-branca Piptadenia stipulacea (Benth Ducke, frente a linhagens de bactérias patogênicas. O pó da casca do caule de ambas as espécies foi submetido à avaliação bromatológica e determinados os teores de Matéria Seca, Matéria Mineral, Proteína Bruta, Fibra em Detergente Neutro e Energia Bruta. Os resultados para a prospecção química indicaram a presença de taninos e outros compostos fenólicos, bem como a presença de saponinas em ambos os extratos. Os extratos das duas espécies demonstraram que mais de uma parte das plantas possui atividade antimicrobiana. A composição bromatológica da casca do caule de jurema-preta e jurema-branca apresentou teores diferenciados para as variáveis avaliadas.The need to find new efficient drugs to combat microbes has increased the search for new natural compounds with biological activities. In this work, phytochemical studies and microbiological analysis were carried out with the ethanol extracts of Mimosa tenuiflora (Wild Poiret and Piptadenia stipulacea (Benth Ducke on pathogenic bacteria strains. The bark powders of both species were submitted to bromatologic evaluation and the levels of dry matter, mineral matter, crude protein, neutral detergent fiber (NDF and crude energy were determined. The results of the chemical search chemical showed the presence of tannins and other phenolic compounds as well as the presence of saponins in both extracts. The microbiologic evaluation of the extracts of both species showed that more than one part of the plants had antimicrobial activity. The bromatologic composition of the bark powder of Mimosa tenuiflora (Wild

  10. Crescimento Inicial de Mimosa scabrella Benth., Schinus terebinthifolius Raddi e Allophylus edulis (St. Hil. Radl. sob Diferentes Regimes de Adubação Initial Growth of Mimosa scabrella Benth., Schinus terebinthifolius Raddi e Allophylus edulis (St. Hil. Radl. with Different Levels of Mineral Fertilization

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    Juliane Garcia Knapik

    2011-03-01

    Full Text Available

    O presente trabalho estudou a produção de mudas em viveiro envolvendo as espécies Mimosa scabrella Benth. (bracatinga, Schinus terebinthifolius Raddi (aroeira-vermelha e Allophylus edulis (ST Hil. Radl (vacum. O experimento foi conduzido no Município de Colombo, PR, no viveiro da Embrapa Florestas. Em fevereiro de 2004, foi realizada a semeadura das três espécies, em tubetes com cerca de 50 cm3, preenchidos com 70% de substrato comercial a base de casca de pínus e vermiculita e 30% de fibra de coco granulada. Os tratamentos corresponderam à três diferentes dosagens de adubos, parceladas em adubação de base e de cobertura, dispostos em blocos ao acaso, com parcelas de nove mudas e sete repetições, correspondendo a 63 mudas por espécie/tratamento. Aos quatro meses após a semeadura, foi realizada a avaliação do experimento, com medições de altura e diâmetro o caule de todas as mudas, e selecionada uma muda por repetição para as análises destrutivas de biomassa seca (parte aérea e radicial, tendo como critério a escolha da muda mais próxima da média da repetição. A aroeira-vermelha foi a espécie que melhor respondeu à adubação, seguida da bracatinga. Diferentemente das demais espécies, o vacum apresentou baixa exigência quanto à adubação no processo de produção de mudas. O crescimento das espécies pode ter sido mais lento devido à época do ano, que envolveu o inverno. 
    In order to study the influence of mineral nutrition on quality and the growth of��seedlings of Schinus terebinthifolius Raddi, Mimosa scabrella Benth. and Allophylus edulis (ST. Hil. Radl, a experiment was conducted in the nursery facilities of Embrapa Florestas, Colombo – PR. The three species were sown in plastic containers of 50 cm3 in February 2004. The substrate was a mixture of a commercial formula (pine bark and vermiculite and granulated coconut fiber in a proportion of 70% and 30

  11. Colonização micorrízica e nodulação radicular em mudas de sabiá (Mimosa caesalpiniaefolia Benth. sob diferentes níveis de salinidade Mycorrhizal colonization and root nodulation in sabiá seedlings (Mimosa caesalpiniaefolia Benth. at different salinity levels

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    Rodrigo Castro Tavares

    2012-09-01

    Full Text Available O sabiá (Mimosa caesalpiniaefolia Benth. é uma espécie vegetal nativa do nordeste brasileiro e reúne algumas características fundamentais para compor programas de reabilitação de áreas salinizadas, principalmente quanto associado aos fungos micorrízicos arbusculares (FMAs e a bactérias fixadoras de nitrogênio (BFN. O objetivo do presente estudo foi avaliar a colonização micorrízica e a nodulação radicular de mudas de sabiá adubadas com composto orgânico e irrigadas com águas de diferentes condutividades elétricas. O experimento foi conduzido em casa de vegetação, com delineamento experimental inteiramente casualizado, em esquema fatorial com 2 (presença e ausência de fungos micorrízicos arbusculares x 2 (presença e ausência de composto orgânico x 5 níveis de condutividade elétrica da água de irrigação (0,7; 1,2; 2,2; 3,2 e 4,2 dS m-1, com 3 repetições. Os resultados obtidos indicam que: a salinidade reduziu a colonização micorrízica e a nodulação radicular das mudas de sabiá; a intensificação das condições de estresse salino aumentaram a dependência micorrízica das mudas de sabiá; a colonização das mudas de sabiá com os FMAs proporcionou aumentos na matéria seca dos nódulos radiculares da ordem de 1900%; as micorrizas arbusculares reduziram o pH após o cultivo do solo; e a adição de vermicomposto não promoveu efeito sobre a colonização micorrízica das mudas de sabiá, entretanto, aumentou a produção de matéria seca dos nódulos radiculares.The sabiá (Mimosa caesalpiniaefolia Benth. is a plant species native to the Brazilian northeast and brings together some fundamental features for use in rehabilitation programs of salinized areas, especially if associated with arbuscular mycorrhizal fungi (AMF and nitrogen-fixing bacteria (BFN. The aim of this study was to evaluate the mycorrhizal colonization and root nodulation of sabiá seedlings fertilized with organic compost and irrigated

  12. Estudo fitoquímico e análise mutagênica das folhas e inflorescências de Erythrina mulungu (Mart. ex Benth. através do teste de micronúcleo em roedores Phytochemical and mutagenic analysis of leaves and inflorescences of Erythrina mulungu (Mart. Ex Benth through micronucleus test in rodents

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    A.P De Bona

    2012-01-01

    Full Text Available Este trabalho teve como objetivo investigar a composição química, estabelecer a dose letal média (DL50 e avaliar os potenciais efeitos mutagênicos do extrato hidroalcoólico de folhas e inflorescências de Erythrina mulungu Mart. ex Benth por meio do teste de micronúcleo em medula óssea de camundongos. Os ensaios fitoquímicos foram realizados através de reações preliminares com mudança de coloração e/ou formação de precipitado; a DL50, por meio da administração intraperitoneal de três concentrações dos extratos, avaliando-se o número de óbitos após 48 horas e o teste de micronúcleo foi feito por meio do método do esfregaço, após exposição dos animais a cinco dias de tratamento. Os resultados fitoquímicos demonstraram presença de açúcares redutores, fenóis e taninos, proteínas e aminoácidos, flavonóides, alcalóides, depsídeos e depsidonas e derivados de cumarina em ambos os órgãos; saponinas espumídicas e esteróides e triterpenóides nas folhas e glicosídeos cardiotônicos e antraquinônicos e alcalóides nas inflorescências. Para a DL50 a folha demonstrou-se atóxica e a inflorescência moderadamente tóxica. Para o teste de micronúcleo, os resultados indicaram ausência de citotoxicidade e genotoxicidade dose-dependente para as folhas e independente da dose para as inflorescências. Assim, esses resultados sugerem que a planta, nas condições analisadas, possui potencial para induzir danos ao DNA.This study aimed to investigate the chemical composition, to establish the mean lethal dose (LD50 and to assess the potential mutagenic effects of hydroalcoholic extract of leaves and inflorescences of Erythrina mulungu Mart. ex Benth by using micronucleus test in bone marrow of mice. Phytochemical assays were carried out through preliminary reactions with color change and/or precipitate formation; the LD50 was obtained by intraperitoneal administration of three concentrations of the extracts, assessing

  13. Composição química de forrageiras e seletividade de bovinos em bosque-de-sabiá (Mimosa caesalpiniifolia Benth. nos períodos chuvoso e seco Chemical composition of forage and selectivity by bovines of "sabiá" (Mimosa caesalpiniaefolia Benth. in the rainy and dry seasons

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    Ednéia de Lucena Vieira

    2005-10-01

    Full Text Available A pesquisa foi desenvolvida no período de junho de 1999 a março de 2000, com o objetivo de determinar, nos períodos chuvoso e seco, a composição química de sabiá (Mimosa caesalpiniifolia Benth., com acúleo e sem acúleo, na dieta de bovinos em condições de pastejo e a composição botânica do bosque de sabiá e da dieta selecionada. Não foi observada diferença significativa quanto à composição química entre os sabiás com e sem acúleo, obtendo-se valores médios, no período chuvoso, de 26,45% de matéria seca (MS, 27,63% de proteína bruta (PB, 44,39% de fibra em detergente neutro (FDN, 25,80% de fibra em detergente ácido (FDA, 1,24% de cálcio (Ca, 0,22% de fósforo (P, 1,63% de potássio (K e 1,12% de sódio (Na. No período seco, os valores médios observados foram de 42,39% de MS; 19,30% de PB; 39,05% de FDN; 21,83% de FDA; 1,25% de Ca; 0,15% de P; 1,63% de K e 0,27% de Na. Para a composição botânica da dieta, observou-se que, durante o período chuvoso, as amostras de extrusa apresentaram maior participação de sabiá (83,1% que no período seco ( 12,3%. Os resultados obtidos indicaram que, durante o período chuvoso, os animais selecionaram sabiá em maior quantidade para sua dieta e que a coleta manual, como realizada, superestimou a fração fibrosa da dieta calculada a partir da composição química da extrusa.This trial was carried out from June 1999 to March 2000 to evaluate the chemical composition of samples of sabiá legume (Mimosa caesalpiniaefolia Benth., with and without thorns, collected in the rainy and dry seasons and to compare the botanical composition of sabiá pasture with that of the diet selected by the grazing animals. No significant difference was observed in chemical composition between samples of sabiá with and without thorns. The mean values obtained in the rainy samples were: 26.45% drymatter (DM, 27.63% crude protein (CP, 44.39% neutral detergent fiber (NDF, 25.80% acid detergent fiber

  14. Deposição e acúmulo de matéria seca e nutrientes em serapilheira em um bosque de sabiá (Mimosa caesalpiniifolia Benth. Litter dry mass and nutrient deposition and accumulation in a sabiá (Mimosa caesalpiniifolia Benth. woodlot

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    Rinaldo Luiz Caraciolo Ferreira

    2007-02-01

    Full Text Available A quantificação da deposição de serapilheira e o acúmulo de manta orgânica são etapas importantes dos estudos de ciclagem de nutrientes. Com esse fim, objetivou-se quantificar a deposição de serapilheira e o acúmulo de manta orgânica em um povoamento de Sabiá (Mimosa caesalpiniifolia Benth., no campo experimental pertencente à Empresa Pernambucana de Pesquisa Agropecuária - IPA, Itambé, PE. Para quantificação da serapilheira foram utilizados 20 coletores de madeira de 0,50 x 0,50 x 15 cm, à distância do solo de 30 cm, distribuídos sistematicamente na área. O material depositado foi coletado mensalmente no período de outubro/2000 a setembro/2001. O material colhido em cada coletor foi separado em folhas, galhos, flores, frutos e miscelânea. A manta orgânica não decomposta sobre o solo foi amostrada, ao acaso, nas proximidades da caixa coletora, obtendo-se 20 amostras mensais. De cada amostra foi retirado o material contido numa área de 30 x 30 cm. O material colhido foi separado em folhas, flores, frutos, miscelânea e galhos. As amostras foram levadas à estufa a 60 º C no período de 48 horas até peso constante. A deposição mensal de serapilheira e frações folha e legume ocorreu em outubro, provavelmente devido ao início do período seco. A deposição e o estoque de serapilheira foram estimados em 7830,44 kg.ha-1.ano-1 e 8906.9, kg.ha-1, respectivamente, com a fração folha dominando. A fração folha apresentou os maiores teores de nutrientes.Quantification of litter deposition and organic mantle accumulation are important steps on nutrient cycling studies. This study aimed to quantify litter deposition and organic mantle in a sabiá (Mimosa caesalpiniifolia Benth woodlot in an experimental farm in the Brazilian northeast region. Twenty 0.50 x 0.50 x 0.15 m wood collectors, to quantify litter deposition, were systematically distributed over the area elevated 30 cm above the soil. Deposited material was

  15. Efeitos potencialmente alelopáticos dos óleos essenciais de Piper hispidinervium C. DC. e Pogostemon heyneanus Benth sobre plantas daninhas Potentially allelopathic effects of the essential oils of Piper hispidinervium C. DC. and Pogostemon heyneanus (Benth on weeds

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    Antônio Pedro da Silva Souza Filho

    2009-01-01

    Full Text Available Este trabalho teve por objetivo caracterizar a atividade potencialmente alelopática de óleos essenciais de pimenta longa (Piper hispidinervium C. DC. e oriza (Pogostemon heyneanus Benth e analisar, comparativamente, seus efeitos alelopáticos. Óleos essenciais obtidos foram preparados em concentrações de 0,25, 0,5 e 1,0%, tendo como eluente o éter metílico, e testados sobre a germinação de sementes, desenvolvimento da radícula e do hipocótilo das plantas daninhas de área de pastagens cultivadas, malícia (Mimosa pudica e mata-pasto (Senna obtusifolia. Os óleos das duas espécies evidenciaram atividade alelopática em intensidades que variaram em função da concentração do óleo, da espécie doadora, da planta receptora e do fator da planta analisado. A germinação das sementes foi o fator mais intensamente inibido pelos óleos. As intensidades das inibições estiveram positivamente associadas à concentração, com inibições máximas verificadas a 1,0%. Malícia foi à espécie receptora mais sensível aos efeitos do óleo. Comparativamente, o óleo essencial da pimenta longa revelou maior potencial para inibir a germinação e o desenvolvimento das duas plantas receptoras, notadamente em relação à germinação de sementes, quando as diferenças foram mais marcantes. Os resultados foram atribuídos à composição química dos óleos, especialmente em relação à presença de monoterpenos, monoterpenos oxigenados e sesquiterpenos.This study aimed to characterize the potential allelopathic activity of essential oils of pimenta longa (Piper hispidinervium C. DC. and oriza (Pogostemon heyneanus Benth, and to examine, comparatively, their allelopathic effects. Essential oils obtained were prepared at concentrations of 0.25%, 0.5% and 1.0%, with ether methanol as eluent, and tested upon the germination of seeds and the development of the radicle and the hypocotyl of the weeds of cultivated grassland areas, malícia (Mimosa

  16. Efeito alelopático de folhas de bamburral [Hyptis suaveolens (L. Poit.] sobre a germinação de sementes de sorgo (Sorghum vulgare Pers., rabanete (Raphanus sativus L. e alface (Lactuca sativa L. Allelopathic effects of leaves of "bamburral" [Hyptis suaveolens (L. Poit.] on the germination of seeds of sorghum (Sorghum vulgare Pers. , radish (Raphanus sativus L. and lettuce (Lactuca sativa L.

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    A.C. Rodrigues

    2012-01-01

    Full Text Available O objetivo do presente trabalho foi à verificação do efeito alelopático de Hyptis. suaveolens na germinação de sorgo, alface e rabanete, bem como, a comprovação da existência de compostos com potencial alelopático. Sementes de sorgo, alface e rabanete foram semeadas em substrato constituído de areia, terra e adubo orgânico contendo folhas de H. suaveolens. As análises da germinação foram feitas considerando a protrusão da radícula para o término do evento germinativo. Foi calculado o IVG (índice de velocidade de germinação e %G (porcentagem de germinação. Os resultados mostraram que sorgo e a alface foram mais susceptíveis ao potencial alelopático de H. suaveolens, sendo que para o rabanete foi observado um efeito benéfico. Entre os tratamentos, o substrato esterilizado e não esterilizado mostraram diferenças entre si. A análise cromatográfica do óleo essencial presente nas folhas de H. suaveolens revelou a presença de compostos com potencial alelopático. Portanto, H. suaveolens, pode apresentar efeito alelopático positivo no IVG de sementes de rabanete e a presença de microorganismos pode ser necessária para que esse efeito alelopático aconteça.The aim of this study was to verify the allelopathic effect of H. suaveolens on the germination of sorghum, lettuce and radish, as well as to prove the existence of compounds with allelopathic potential. Seeds of sorghum, lettuce and radish were sown in substrate consisting of sand, soil and organic fertilizer containing leaves of H. suaveolens. The germination tests were performed considering the protrusion of the radicle for the conclusion of the germinative event. GSI (germination speed index and G% (percentage of germination were calculated. The results showed that sorghum and lettuce were more susceptible to the allelopathic potential of H. suaveolens, while for radishes a beneficial effect was observed. Between treatments, the sterilized and unsterilized

  17. Foliar anatomical study of Thaumatococcus daniellii (Benth.) Benth ...

    African Journals Online (AJOL)

    Foliar, petiole and stem anatomical characters of Thaumatococcus daniellii and Megaphrynium macrostachyum in Ile-Ife and Osogbo, environs, Osun State in Nigeria are reported. The aim is to determine the macro and micro-characters that could facilitate their identification and classification. It is envisaged that this will ...

  18. Foliar anatomical study of Thaumatococcus daniellii (Benth.) Benth ...

    African Journals Online (AJOL)

    useful in roof thatching as well as making cushion for sleeping mats (Terashima and. Ichikawa, 2003). There are other numerous examples of disposable utensils made of ... There is a dark green region along the mid-rib of all M. macrostachyum studied and again they have big and robust size when compared with T.

  19. Foliar anatomical study of Thaumatococcus daniellii (Benth.) Benth ...

    African Journals Online (AJOL)

    (3): 601-612. Dilcher DL. 1974. Approaches to the identification of angiosperm leaf remains. Bot Rev, 40(1): 1–157. Doege SJ. 2003. The role of natural calcium oxalate crystals in plant defense against chewing insects. Inquiry, 4: 88-94.

  20. Estudo comparativo da madeira de Mimosa ophthalmocentra Mart. ex Benth e Mimosa tenuiflora (Willd. Poir. (Fabaceae-Mimosoideae na caatinga nordestina Comparative study of Mimosa ophthalmocentra Mart. ex Benth and Mimosa tenuiflora (Willd. Poir. (Fabaceae-Mimosoideae wood in the caatinga of Northeast Brazil

    Directory of Open Access Journals (Sweden)

    Lazaro Benedito da Silva

    2011-06-01

    Full Text Available Pela importância econômica da madeira de algumas espécies do gênero Mimosa L. ocorrentes na caatinga nordestina e pelo fato de Mimosa ophthalmocentra Mart. ex Benth. (jurema-de-imbira, às vezes, ser confundida com Mimosa tenuiflora (Willd. Poir. (jurema-preta ou vice-versa, este trabalho objetivou apresentar novos dados referentes à anatomia e densidade básica da madeira das duas espécies, visando oferecer subsídio para identificação das duas espécies, identificar caracteres anatômicos da madeira relacionando-os com o ambiente caatinga e apresentar o potencial energético que sua madeira possui. O estudo anatômico e a determinação da densidade da madeira foram realizados com amostras ao nível do peito (1,30 m acima do solo e em dois galhos com diferentes diâmetros, de espécimes ocorrentes nos municípios de Serra Talhada e Sertânia, Pernambuco, Brasil. As espécies apresentam distinção expressa através do tipo de casca, coloração do cerne e do alburno, além das características peculiares da madeira. Mimosa ophthalmocentra apresenta camadas de crescimento distintas, constituídas por linhas de parênquima axial contendo cristais, parênquima axial escasso e menor quantidade de raios por mm². Já Mimosa tenuiflora apresenta camadas de crescimento distintas, porém sem cristais, parênquima axial vasicêntrico, em faixas ou aliforme confluente, e maior percentagem de raios. Ambas apresentam algumas estruturas da madeira com as características anatômicas comuns às diversas espécies do gênero Mimosa, contribuindo assim para uma melhor caracterização do gênero. Pelos parâmetros anatômicos da madeira e pela elevada densidade básica (>0,84 g/cm³, concluiu-se que as duas espécies apresentam perspectivas seguras para a produção de álcool combustível e carvão vegetal desde a fase de lenho juvenil. Mimosa ophthalmocentra apresenta maior potencial energético, pela maior percentagem de fibras e por possuir par

  1. Efeito da temperatura e do teor de umidade na iniciação e desenvolvimento do rizoma de Kohleria eriantha (Benth. Hanst. (Gesneriaceae Effect of temperature and the water content in the initiation and developmental of the rhizome of Kohleria eriantha (Benth. Hanst. (Gesneriaceae

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    Julieta Andrea Silva de Almeida

    2004-12-01

    Full Text Available Kohleria eriantha (Benth. Hanst. pertence à família Gesneriaceae e possui órgão subterrâneo, que está associado à reprodução vegetativa. Este órgão apresenta gemas envoltas por folhas modificadas, as quais armazenam amido. Em seções do rizoma (1,5 a 2,0cm compr. contendo seis gemas, só uma gema geralmente brota. Esta pode diferenciar-se em dois padrões morfológicos: parte aérea ou rizoma. Em seções mantidas em substrato com baixo teor de umidade (1mL de água ou em sua ausência, houve brotação do padrão rizoma, em seções em substrato com elevado teor de umidade (12mL de água, brotação do padrão parte aérea. A temperatura de 20ºC também favoreceu a brotação do padrão rizoma, independente do volume de água do substrato. Seções também desenvolveram o padrão rizoma em substrato com adição de solução de polietilenoglicol 6000 (PEG, nas concentrações de 161,2; 235,2 e 340,0g/L, que geraram os potenciais de -3, -6 e -12 MPa, respectivamente. Seções mantidas em substrato com baixo teor de umidade (1mL de água apresentaram redução de massa seca e elevada concentração osmótica em relação àquelas em substrato com elevado teor de umidade. Verificou-se que a formação do padrão rizoma foi influenciada pelos fatores teor de umidade e temperatura. Sugere-se que a brotação do padrão rizoma foi induzida pelo baixo potencial hídrico nas seções, quando mantidas em substrato com baixo teor de umidade. Além disto, evidenciou-se que as gemas do rizoma de Kohleria eriantha apresentam elevado grau de plasticidade.Kohleria eriantha (Benth. Hanst is a plant belonging to the family Gesneriaceae, with an underground organ, which is associated with vegetative reproduction. This organ is a rhizome, whose stem bears buds covered with modified leaves that store up starch. In small sections of this rhizome, containing six buds (1.5 to 2.0cm long, only one bud sprouted. The sprouted bud could be differentiated

  2. Protective effects of alginate–chitosan microspheres loaded with alkaloids from Coptis chinensis Franch. and Evodia rutaecarpa (Juss. Benth. (Zuojin Pill against ethanol-induced acute gastric mucosal injury in rats

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    Wang QS

    2015-11-01

    Full Text Available Qiang-Song Wang,1,2,* Xiao-Ning Zhu,1,* Heng-Li Jiang,1,* Gui-Fang Wang,3 Yuan-Lu Cui1 1Tianjin State Key Laboratory of Modern Chinese Medicine, Research Center of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, 2Institute of Biomedical Engineering, Chinese Academy of Medical Science & Peking Union Medical College, 3Pharmacy Department, Baokang Hospital, Tianjin University of Traditional Chinese Medicine, Tianjin, People’s Republic of China *These authors contributed equally to this work Abstract: Zuojin Pill (ZJP, a traditional Chinese medicine formula, consists of Coptis chinensis Franch. and Evodia rutaecarpa (Juss. Benth. in a ratio of 6:1 (w/w and was first recorded in “Danxi’s experiential therapy” for treating gastrointestinal disorders in the 15th century. However, the poor solubility of alkaloids from ZJP restricted the protective effect in treating gastritis and gastric ulcer. The aim of the study was to investigate the protective mechanism of mucoadhesive microspheres loaded with alkaloids from C. chinensis Franch. and E. rutaecarpa (Juss. Benth. on ethanol-induced acute gastric mucosal injury in rats. Surface morphology, particle size, drug loading, encapsulation efficiency, in vitro drug release, mucoadhesiveness, and fluorescent imaging of the microspheres in gastrointestinal tract were studied. The results showed that the mucoadhesive microspheres loaded with alkaloids could sustain the release of drugs beyond 12 hours and had gastric mucoadhesive property with 82.63% retention rate in vitro. The fluorescence tracer indicated high retention of mucoadhesive microspheres within 12 hours in vivo. The mucoadhesive microspheres loaded with alkaloids could reduce the gastric injury by decreasing the mucosal lesion index, increasing the percentage of inhibition and increasing the amount of mucus in the gastric mucosa in an ethanol-induced gastric mucosal injury rat model. Moreover, the

  3. Online HPLC-DPPH method for antioxidant activity of Picrorhiza kurroa Royle ex Benth. and characterization of kutkoside by ultra-performance LC-electrospray ionization quadrupole time-of-flight mass spectrometry.

    Science.gov (United States)

    Bhandari, Pamita; Kumar, Neeraj; Singh, Bikram; Ahuja, Paramvir S

    2010-03-01

    Picrorhiza kurroa Royle ex Benth., is widely used in the Indian systems of medicine for the treatment of various liver ailments. Since, the role of oxidative stress in the pathogenesis of liver injury has become generally recognized, in present study the free radical scavenging effect of P. kurroa was assessed by on-line HPLC-DPPH and colorimetric DPPH methods. The comparative study on antioxidant activity of P. kurroa extracts by both methods revealed that colorimetric method showed very less free radical scavenging effect while HPLC-DPPH method showed high activity. Further, the kutkoside, an important ingredient of a potent hepatoprotective formulation "kutkin/picroliv" was investigated for its chemical composition by ultra-performance liquid chromatography coupled with diode array detection/electrospray ionization quadrupole time-of-flight mass spectrometry (UPLC-DAD/ESI-QTOF-MS). Kutkoside was considered to be a single compound and reported as picroside-II or kutkoside, however, present investigation illustrated that kutkoside is a mixture of iridoid glycosides namely, picroside II, picroside IV and 6-ferulloylcatalpol.

  4. 3,6-Dimethoxy-6″,6″-Dimethyl-(7,8,2″,3″)-Chromeneflavone, a Flavonoid Isolated from Lonchocarpus Araripensis Benth. (Fabaceae), Reduces Nociceptive Behaviour in Mice.

    Science.gov (United States)

    Almeida, Jackson R G S; Silva, Juliane C; Guimarães, Amanda L; Oliveira, Ana P; Souza, Grasielly R; Oliveira-Júnior, Raimundo G; Lima-Saraiva, Sarah R G; Barbosa-Filho, José M; Braz-Filho, Raimundo; Queiroz, Dinalva Brito; Botelho, Marco Antônio

    2015-10-01

    Lonchocarpus araripensis Benth. is largely distributed in the northeast region of Brazil. It is popularly known as 'sucupira'. Recent studies have shown that some species of Lonchocarpus have interesting pharmacological activities. In this study, we evaluated the antinociceptive effect of a flavone isolated from L. araripensis. The chemical examination resulted in the isolation of 3,6-dimethoxy-6″,6″-dimethyl-(7,8,2″,3″)-chromeneflavone (DDF). The structure of the compound was established by spectral analysis. Antinociceptive activity of DDF was evaluated by measuring nociception by acetic acid, formalin and hot plate tests. The rota rod test was used to evaluate motor coordination. The results demonstrated that DDF was able to prevent acetic-acid-writhing-induced nociception (p DDF produced a significant reduction of the nociceptive behaviour at the early and late phases of paw licking in the formalin test. Also, DDF produced an inhibition of the nociceptive behaviour during a hot-plate test. No alteration in motor coordination was observed. These results confirm the hypothesis that DDF reduces the nociceptive behaviour in mice, probably through central mechanisms, but without compromising the motor coordination of animals. Copyright © 2015 John Wiley & Sons, Ltd.

  5. Efeito alelopático do extrato aquoso de sabiá na germinação de sementes de fava Allelopathic effect of aqueous extract of Mimosa caesalpiniaefolia Benth. in seed germination of Phaseolus lunatus

    Directory of Open Access Journals (Sweden)

    Elane Grazielle Borba de Sousa Ferreira

    2010-09-01

    Full Text Available O sabiá (Mimosa caesalpiniaefolia Benth. é uma planta medicinal que pode ser utilizada em sistemas agroflorestais. É empregada na composição de pastagens arbóreas, em faixas entre plantações, para enriquecer capoeiras e ainda pode ser empregada como cerca viva. A fava (Phaseolus lunatus L. é uma das quatro espécies do gênero Phaseolus exploradas comercialmente, seu consumo é preferencialmente na forma de grãos verdes cozidos ou na forma de conserva. O presente trabalho teve como objetivo verificar o efeito alelopático do extrato aquoso do sabiá sobre a germinação de sementes e crescimento inicial de plântulas de fava. As sementes de fava foram postas para germinar em caixas plásticas, onde foram semeadas entre o substrato vermiculita, e em seguida colocados em germinador a 25 ºC e sob luz contínua. O substrato foi umedecido, com o extrato aquoso de folhas jovens de sabiá, nas concentrações de 25; 50; 75 e 100%, além da testemunha umedecida apenas com água destilada. As variáveis avaliadas foram: porcentagem, primeira contagem e índice de velocidade de germinação além do comprimento da raiz primária. O delineamento experimental utilizado foi o inteiramente casualizado, com quatro repetições de 25 sementes cada. Os dados obtidos foram submetidos à análise de variância e de regressão polinomial. No estudo da regressão polinomial foi empregada a equação que melhor se ajustou aos dados. Os valores em porcentagem foram transformados em arc sen (n/1000,5. Conclui-se que as diferentes concentrações do extrato de folhas jovens de sabiá utilizadas não prejudicaram a germinação das sementes de fava.The Mimosa caesalpiniaefolia Benth. is a medicinal plant that can be used in agroforestry systems, is also employed in the composition of pasture trees in strips between fields, to enrich brush fields and as a hedge. The Phaseolus lunatus L. is one of four species of the genus Phaseolus exploited commercially; its

  6. Aporte e decomposição de serapilheira em áreas de floresta secundária, plantio de sabiá (Mimosa caesalpiniaefolia Benth. e andiroba (Carapa guianensis Aubl. na Flona Mário Xavier, RJ.

    Directory of Open Access Journals (Sweden)

    Milton Marques Fernandes

    2010-08-01

    Full Text Available Este trabalho teve como objetivo avaliar o aporte e a decomposição da serapilheira em áreas de floresta secundária espontânea, plantio de sabiá (Mimosa caesalpiniaefolia Benth. e plantio de andiroba (Carapa guianensis Aubl.. Para avaliação do aporte de serapilheira, foram instalados dez coletores cônicos sendo o aporte avaliado durante o período de outubro de 2003 a setembro de 2004. Posteirormente, foi feita a quantificação dos macronutrientes (N, P e K. A avaliação da taxa de decomposição foi realizada utilizando-se o método dos litter bags. A área de plantio de andiroba aportou a maior quantidade de serrapilheira 9,20 Mg ha-1, sendo seguida pelo plantio de sabiá com 9,06 Mg ha-1 e pela floresta secundária espontânea 7,63 Mg ha-1 por ano. A serapilheira das áreas de plantio de sabiá e do plantio de andiroba apresentou maiores valores de nitrogênio e fósforo que a área de floresta secundária. A velocidade de decomposição da serapilheira foi maior nas áreas de plantio de sabiá e andiroba, podendo tal comportamento ter sido influenciado pelo maior conteúdo de nitrogênio na serapilheira. O fósforo foi o nutriente que apresentou maior velocidade de liberação.

  7. Protective effect of Rabdosia amethystoides (Benth Hara extract on acute liver injury induced by Concanavalin A in mice through inhibition of TLR4-NF-κB signaling pathway

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    Ke-Feng Zhai

    2016-02-01

    Full Text Available Extract of Rabdosia amethystoides (Benth Hara (ERA, a traditional Chinese medicine has antibacterial, antiviral, anti-tumor, anti-hepatitis and anti-inflammatory properties. However, the hepatoprotective effects and molecular mechanisms of ERA on acute liver injury have not been fully elucidated. This study aims to investigate the anti-inflammatory effect and liver protection of ERA against the acute liver injury induced by Concanavalin A (Con A and its underlying molecular mechanisms in mice. Mice received ERA (50, 100, 150 mg/kg body weight by gavage before Con A intravenous administration. We found that ERA pretreatment was able to significantly reduce the elevated serum alanine and aspartate aminotransferase levels and liver necrosis in Con A-induced hepatitis. In addition, ERA treatment significantly decreased the myeloperoxidase, malondialdehyde levels and augmented superoxide dismutase level in the liver tissue, and also suppressed the secretion of proinflammatory cytokines in the serum, compared with Con A group by enzyme linked immunosorbent assay. Furthermore, we observed that ERA pretreatment can significantly decrease the expression level of Toll-like receptor (TLR 4 mRNA or protein in liver tissues. Further results showed that ERA pretreatment was capable of attenuating the activation of the NF-κB pathway by inhibiting IκBα kinase and p65 phosphorylation in Con A-induced liver injury. Our results demonstrate that ERA pretreatment has hepatoprotective property against Con A-induced liver injury through inhibition of inflammatory mediators in mice. The beneficial effect of ERA may be mediated by the downregulation of TLR4 expression and the inhibition of NF-κB activation.

  8. Biometria, caracterização física e rendimento lipídico do fruto de Licania rigida Benth adquiridos no município de Pombal-PB

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    T. S. S. Almeida

    2015-12-01

    Full Text Available Resumo: Licânia rigida Benth, popularmente oiticica, é uma vegetação típica no sertão nordestino. Destaca-se pelo alto teor de óleos em seus frutos e boa produtividade mesmo em épocas de seca, sendo capaz de promover desenvolvimento social e econômico para a região, pela utilização industrial de tintas, vernizes, sabão, lonas e esmaltes finos. Portanto, objetiva-se no presente estudo determinar a biometria, parâmetros físicos e rendimento lipídico do fruto da oiticica. Os frutos foram colhidos, selecionados, sanitizados. Em seguida, utilizou-se um paquímetro para a realização da biometria dos frutos e então sofreram separação em exocarpo-mesocarpo, endocarpo e amêndoas. As amostras foram trituradas e armazenadas ate procedidas as análises. Para a determinação do pH e condutividade, utilizou-se cerca de 5g de cada amostra, acrescidos de 50mL de água destilada e homogeneização, por conseguintemente foi utilizado o peagâmetro digital e condutivímetro, respectivamente. Para a acidez total titulável (ATT, utilizou-se as amostras anteriores e titulou-se com NaOH 0,1N, usando fenolftaleína como indicador, até as amostras alcançarem pH de 8,1. Procedeu-se a determinação do teor lipídico pelo método de Sohlext, com 2 a 5 g das amostras e hexano como solvente. Os frutos apresentaram peso médio de 9g. A variação do pH para as diferentes amostra foi irrelevante, enquanto o exocarpo-mesocarpo apresentou maior condutividade, com 308,6 mScm-1, e o endocarpo foi evidenciado por apresentar maior ATT, com 16,8%. Obteve-se na amêndoa maior percentual lipídico, com rendimento médio de 43,0%. Diante da importância e alta rentabilidade lipídica do fruto, faz-se necessário desenvolvimento de metodologias para utilização dessa matéria-prima.Biometrics, physical characteristics and yield fruit of lipid Licania rigid Benth purchased in the municipality of Pombal-PBAbstract: Licania rigid Benth, popularly oiticica, is

  9. Gastroprotective Mechanisms of the Monoterpene 1,8-Cineole (Eucalyptol.

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    Germana Freire Rocha Caldas

    Full Text Available Recently, our research group identified and reported 1,8-cineole (CIN, a monoterpene that naturally occur in many aromatic plants, as one of the major constituent of the essential oil from leaves of Hyptis martiusii (EOHM, as well as characterized the gastroprotective action of this oil. The aim of this study was to investigate the mechanisms of action involved in the antiulcer and healing activity of CIN, in order to confirm its correlation with the gastroprotective effect of EOHM. Wistar rats were exposed to different protocols (acute ulceration, gastrointestinal motility and antisecretory activity. In addition, were determinated the involvement of nitric oxide and sulphydryl groups; the levels of gastric mucus, lipid peroxidation, sulphydryl groups and myeloperoxidase activity. The healing ability was evaluated by acetic acid-induced chronic ulcer and histological and immunohistochemical analysis (PCNA, Ki-67 and BrdU. The treatment with CIN inhibited ethanol-, ethanol/HCl- and indomethacin-induced gastric lesions. The highest doses of CIN inhibited gastric emptying, but did not affect intestinal transit. CIN (100 mg/kg reduced the volume of basal but not stimulated acid secretion. CIN increased levels of mucus (89.3%, prevented depletion of -SH groups (62.6% and reduced the level of lipid peroxidation (55.3% and myeloperoxidase activity (59.4% in the gastric mucosa. In chronic ulcer model, CIN reduced in 43.1% the gastric area lesion, promoted significant regeneration and restoration of the levels of mucus in glandular cells as confirmed by histological analysis; and promoted increase in cell proliferation as evidenced by reactivity for PCNA, Ki-67 and BrdU. This findings demonstrate the role of 1,8-cineole as an important ulcer healing agent and indicate the involvement of antioxidant and cytoprotective mechanisms in the gastroprotective effect of compound. This study also provides evidence that 1,8-cineole is related to the

  10. Gastroprotective Mechanisms of the Monoterpene 1,8-Cineole (Eucalyptol).

    Science.gov (United States)

    Rocha Caldas, Germana Freire; Oliveira, Alisson Rodrigo da Silva; Araújo, Alice Valença; Lafayette, Simone Sette Lopes; Albuquerque, Giwellington Silva; Silva-Neto, Jacinto da Costa; Costa-Silva, João Henrique; Ferreira, Fabiano; Costa, José Galberto Martins da; Wanderley, Almir Gonçalves

    2015-01-01

    Recently, our research group identified and reported 1,8-cineole (CIN), a monoterpene that naturally occur in many aromatic plants, as one of the major constituent of the essential oil from leaves of Hyptis martiusii (EOHM), as well as characterized the gastroprotective action of this oil. The aim of this study was to investigate the mechanisms of action involved in the antiulcer and healing activity of CIN, in order to confirm its correlation with the gastroprotective effect of EOHM. Wistar rats were exposed to different protocols (acute ulceration, gastrointestinal motility and antisecretory activity). In addition, were determinated the involvement of nitric oxide and sulphydryl groups; the levels of gastric mucus, lipid peroxidation, sulphydryl groups and myeloperoxidase activity. The healing ability was evaluated by acetic acid-induced chronic ulcer and histological and immunohistochemical analysis (PCNA, Ki-67 and BrdU). The treatment with CIN inhibited ethanol-, ethanol/HCl- and indomethacin-induced gastric lesions. The highest doses of CIN inhibited gastric emptying, but did not affect intestinal transit. CIN (100 mg/kg) reduced the volume of basal but not stimulated acid secretion. CIN increased levels of mucus (89.3%), prevented depletion of -SH groups (62.6%) and reduced the level of lipid peroxidation (55.3%) and myeloperoxidase activity (59.4%) in the gastric mucosa. In chronic ulcer model, CIN reduced in 43.1% the gastric area lesion, promoted significant regeneration and restoration of the levels of mucus in glandular cells as confirmed by histological analysis; and promoted increase in cell proliferation as evidenced by reactivity for PCNA, Ki-67 and BrdU. This findings demonstrate the role of 1,8-cineole as an important ulcer healing agent and indicate the involvement of antioxidant and cytoprotective mechanisms in the gastroprotective effect of compound. This study also provides evidence that 1,8-cineole is related to the gastroprotective effect of

  11. AVALIAÇÃO FÍSICO-QUÍMICA E ENERGÉTICA DA MADEIRA DAS ESPÉCIES Piptadenia stipulacea (Benth. Ducke E Amburana cearensis (Allemao A. C. Smith DE OCORRÊNCIA NO SEMIÁRIDO NORDESTINO BRASILEIRO

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    Antonio Marcos César de Almeida

    2015-01-01

    Full Text Available ‘Caatinga’ is an exclusive Brazilian biome, which features a wide variety of fauna and flora, and a good part of these are endemic varieties. Over the years, the abolition of ‘Caatinga’ by human activities has greatly reduced this biome. This fact is mainly due to the use of the energy potential of plant species without proper concern about the sustainability of it. A better understanding of the energy potential of plant species of ‘Caatinga’ enables a more appropriate management. The objective of this study was to evaluate the potential energy and the physical and chemical characteristics of wood species Amburana cearensis (Germans A. C. Smith and Piptadenia stipulacea (Benth. Ducke. Five trees of each species were felled, randomly sampled in ‘São Bento’ site, located in the city of Patos, Paraíba state. The trees were identified and transported to the Department of Forest Product Technology, Federal University of Campina Grande. From each tree, small discs were removed (5 cm thick to 0 (base, 25, 50, 75 and 100% of the commercial height of the trunk (up to 5 cm in diameter and large disks (15 cm thick before and after each small disk, the small discs were divided into four parts, wedge-shaped, passing through the medulla. Two opposing wedges were used to determine the density and the rest was reserved for the physical and chemical wood analyses and the wood discs were destined for greater achievements of carbonizations. Basic density was determined according to the method of hydrostatic balance and to determine the basic density of each tree used as a weighting factor the volume between sections of each disk. After air drying, the samples for chemical analysis were transformed into sawdust and made quantitative determinations of total extractives, lignin, ash and holocellulose content was estimated by difference from the initial mass [100% - (content total extractives + lignin + ash]. Samples intended for carbonizations were

  12. Análise do Crescimento de Hyptis suaveolens Growth Analysis of Hyptis suaveolens

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    R. Gravena

    2002-08-01

    Full Text Available O crescimento de H. suaveolens foi estudado em casa de vegetação, no delineamento experimental inteiramente casualizado, com quatro repetições. As plantas cresceram em vasos com capacidade de cinco litros, preenchidos com areia e irrigados com solução nutritiva, diariamente. Os atributos de crescimento das plantas foram avaliados dos 20 até os 160 dias após a emergência (DAE da planta daninha, em intervalos regulares de 14 dias. A planta atingiu o máximo estimado de biomassa seca acumulada aos 145 DAE. A partir da emergência, até 104 dias, as folhas apresentaram maior participação no acúmulo de biomassa seca total da planta; posteriormente, os caules passaram a deter maior proporção dessa característica. A taxa de assimilação líquida foi crescente do início do ciclo até a época estimada de 71 DAE, decrescendo a seguir, provavelmente devido ao auto-sombreamento das folhas. A análise do crescimento indicou que H. suaveolens pode apresentar alta capacidade competitiva, podendo ser considerada planta infestante de crescimento tardio, quando presente nas culturas anuais de verão.The growth of H. suaveolens was evaluated in greenhouse conditions, using a randomized design, with four replications. The plants were grown in 5-liter pots filled with sand and irrigated daily with a nutritive solution. The growth atributes were evaluated from 20 to 160 days after weed emergence (DAE, at 14-day regular intervals. The maximum estimated dry biomass accumulation of the plant occurred at 145 DAE. Until 104 DAE, the greatest amount of dry matter was partitioned to the leaves. After 104 DAE, most of the dry matter was partitioned to the stems. The net assimilation rate (NAR increased from the beginning of the weed cycle to 71 DAE. After this period, the NAR decreased, probably due to the self-shading of the leaves. Growth analysis indicated that H. suaveolens could be highly competitive, and based on its growth habit, it can be considered a late growth weed in the annual summer crop cycle.

  13. Anatomia do lenho de cinco espécies comercializadas como 'Sucupira'

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    Warley Felício Soares

    Full Text Available A análise comparativa da anatomia do lenho das espécies Bowdichia nitida Spruce ex Benth, Diplotropis brasiliensis (Tul. Benth., Diplotropis martiusii Benth., Diplotropis purpurea (Rich. Amshoff e Diplotropis racemosa (Hoehne Amshoff é descrita no presente trabalho, visando a determinar as principais características anatômicas necessárias à distinção dessas espécies. Qualitativamente, as características anatômicas mais importantes para a diferenciação do lenho dessas espécies foram: camadas de crescimento, estratificação dos raios, arranjo dos vasos e fileira marginal dos raios. Quantitativamente, a análise estatística mostrou que os parâmetros anatômicos mais importantes no agrupamento das espécies foram: diâmetro dos elementos de vasos, largura dos raios em micrometros, comprimento, espessura da parede e largura das fibras. Verificou-se que a anatomia do lenho pode ser utilizada como importante subsídio na identificação das espécies estudadas.

  14. Avaliação da ação espasmolítica do flavonoide 3,6-Dimetil éter galetina, isolado de Piptadenia stipulacea (Benth.) Ducke e investigação do mecanismo de ação em traqueia de cobaia e aorta de rato

    OpenAIRE

    Macêdo, Cibério Landim

    2012-01-01

    O flavonoide 3,6-dimetil éter galetina (FGAL) foi isolado das partes aéreas de Piptadenia stipulacea (Benth.) Ducke e foi investigada sua possível atividade hemolítica em eritrócitos de rato e espasmolítica em vários músculos lisos isolados como útero de rata, íleo e traqueia de cobaia, e aorta de rato. FGAL não causou efeito hemolítico em eritrócitos de ratos, o que é sugestivo de baixa toxicidade. Na triagem farmacológica preliminar realizada em músculos lisos, FGAL apresentou efeito espasm...

  15. Cultivos celulares de Choiba Dipteryx oleifera Benth

    National Research Council Canada - National Science Library

    Murillo Gomez, Paola A; Atehortua, Lucia

    2013-01-01

    ... por sus frutos. Su almendra almacena una buena cantidad de aceites con potencial para la industria alimentaria, lo que podria resultar en una nueva fuente alimenticia, por lo cual el cultivo in vitro de vegetales...

  16. (Jacq) Benth (Mimosaceae) root: Hemoglobin polymerization ...

    African Journals Online (AJOL)

    Results: All the solvent-partitioned fractions of C. portoricensis showed a high percentage inhibition of hemoglobin polymerization at 5 mg/mL with the ethyl acetate fraction showing the highest percentage inhibition of 98.97 ± 1.62 % The pooled chromatographic fraction C3 containing two compounds (Rf value: 0.71, 0.83); ...

  17. SERIANTHES Benth. (Leguminosae-Mimosoideae-Ingeae

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    F. R. FOSBERG

    2015-10-01

    Full Text Available The genus Serianthes was erected by Bentham to accomodate plants which Wallich had called Inga grandiflora, from Singapore, and those which Bertero had called Acacia myriadenia, from Tahiti. It has been accepted from the first, as it is amply distinct from Acacia and reasonably so from Inga. though it is usually placed in the tribe Ingeae. Serianthes is widespread in the southwest Pacific, usually, though not always, occurring on islands, frequently on calcareous or serpentine rocks or their derived soils. It is seldom abundant, though in places common. In habit it varies from a dwarf tree or large shrub to a forest giant. It is reported to be an excellent timber tree, but seems nowhere to be abundant enough to be important commercially. Since Bentham's original description in 1844, eleven additional species and one variety have been ascribed to it in addition to the original S. grandiflora. Three of these do not belong in Serianthes. Most of the specimens in herbaria, excepting those called S. grandiflora, have been misidentified, usually being placed in S. myriadenia. Although I first saw this genus growing and collected it in Raivavae and Rurutu in 1934, my interest in it was aroused by failure, in 1950, to find a satisfactory disposition for specimens collected in Palau, similar to those that Kanehira had referred to S. grandiflora, which they clearly were not. The more I tried to make sense of the herbarium material available in a number of herbaria the more frustrating the problem became. Notes were accumulated in a most haphazard and unsystematic manner, as I had no intention of doing more than identifying my Micronesian material. Finallyit became evident that over half of the entities discernible in the material examined were undescribed, and I felt a certain obligation to work up at least a synopsis of the genus.

  18. Essential Oil of Otostegia integrifolia Benth: Composition ...

    African Journals Online (AJOL)

    The antifungal activity of the oil was either comparable to or better than griseofulvin against most of the fungal pathogens tested. The study provides evidence for an excellent broadspectrum antimicrobial and significant antioxidant activity of O. integrifolia essential oil, a possible explanation for the traditional use of the plant.

  19. Antinociceptive potential of Parkia platycephala Benth. in ...

    African Journals Online (AJOL)

    AcOEt 50 mg/kg presented antinociceptive effect in the late phase of formalin test. These findings indicate that E.EtOH and F.AcOEt showed analgesic actions in diabetic rats. Key words: Parkia platycephala, diabetic neuropathy, tactile allodynia, ...

  20. Hypoglycemic Potential of Verbesina Encelioides Benth. Roots ...

    African Journals Online (AJOL)

    Blood sugar level was determined using digital glucometer. The oral administration of roots extracts at doses of 400 mg/ kg lead to a significant blood glucose reduction in normal and in Streptozotocin, alloxan diabetic mices significantly within 4h. Continued, daily administration of the drug produced a sustained effect.

  1. Antimicrobial Activity Of Coleus aromaticus (Benth) Against ...

    African Journals Online (AJOL)

    Candida krusei showed the highest zone of inhibition of growth, followed by Candida albicans, Proteus mirablis, Escherichia coli, Staphylococcus aureus , Enterococcus faecalis , Klebsiella pneumoniae and the least inhibition was observed for Neisseria gonohorreae. Coleus aromaticus exhibits an effective antifungal and ...

  2. Seleção e melhoramento do timbó: II - Estudo de uma população de 153 plantas de timbó macaquinho - Derris nicou (benth.

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    Luiz O. T. Mendes

    1960-01-01

    Full Text Available Para fins de seleção foi estudada, no Instituto Agronômico do Norte, uma população de 153 plantas de timbó macaquinho - Derris nicou (Benth.. Depois de colhidas as raízes e postas a secar ao ar, foram analisadas para determinação de umidade, cinzas, extratos totais (etéreo e por gravimetria, rotenona + deguelina (por colorimetria e rotenona (por gravimetria. Pelo estudo dos dados pôde-se verificar que havia dois grupos distintos de plantas: um constituído por 82 plantas (POPULAÇÃO A contendo menos de 5% de rotenona nas raízes e outro, com 71 plantas (POPULAÇÃO B, com 9% ou mais de rotenona em suas raízes. Essas populações foram estudadas separadamente. Para um estudo mais detalhado dêsses grupos de plantas também foram calculados o peso líquido das raízes, bem como o rendimento líquido, por planta e em média, em extratos totais, rotenona + deguelina e rotenona. Foram também calculados os teores em deguelina e rotenona contidos nos extratos totais e outros extratos (que não rotenona e deguelina contidos nos extratos totais e, finalmente, a rotenona contida no conjunto rotenona + deguelina. São apresentados os resultados referentes às melhores plantas selecionadas dessa população. Vários fatôres foram levados em consideração para a separação dessas plantas, tidas como os melhores. Os resultados mostram que mediante adequada multiplicação do material selecionado será possível a instalação de culturas de timbó com elevado rendimento em rotenona.A population composed of 153 plants of "timbó macaquinho" (Derris nicou was studied for selection and establishment of high yielding rotenone clones. The roots were air dried and the humidity content determined. Ashes, total extracts, rotenone + degueline and rotenone were determined by chemical analyses. Individual results of the analyses are presented. From the data were calculated the net weight per plant of: a air dried roots; b total extracts; c rotenone

  3. Physical and chemical characterization of honeys produced in the State of Piauí - Brazil

    OpenAIRE

    Silva, Claudécia L. da; Queiroz, Alexandre J. de M.; Figueirêdo, Rossana M. F. de

    2004-01-01

    Este trabalho foi realizado com o objetivo de se caracterizar físico-químicamente méis de Apis mellifera L. produzidos no Estado do Piauí, a partir de floradas típicas da região semi-árida do Nordeste brasileiro e, ao mesmo tempo, avaliar a qualidade desses produtos. Analisaram-se os méis produzidos a partir das floradas de marmeleiro (Croton sonderianus Müll. Arg.), jitirana (Hyptis suaveolens), camaratuba (Cratylia mollis Mart. ex Benth), silvestre (Serjania glabrata) e angico de bezerro (P...

  4. Influência da adubação verde e diferentes adubos orgânicos na produção de fitomassa aérea de atroveran (Ocimum selloi Benth. Influence of green manure and different organic manure on production of aereal phytomass of Ocimum selloi

    Directory of Open Access Journals (Sweden)

    L.A.S. Morais

    2012-01-01

    Full Text Available Este trabalho teve por objetivo verificar o efeito de diferentes adubos orgânicos em associação ou não com adubo verde na produção de folhas de Ocimum selloi Benth., planta nativa do Brasil. O experimento foi conduzido na área experimental da Embrapa Meio Ambiente (CNPMA, localizado no município de Jaguariúna, em duas áreas distintas, sendo uma delas submetidas anteriormente ao plantio e incorporação de adubo verde (Crotalaria juncea. Os tratamentos utilizados foram T1 - testemunha (solo sem adubação, T2 - cama de aviário (5 kg m-2, T3 - hidrolisado de peixe (produto comercial Fishfértil - 5 mL m-2 e T4 - composto orgânico (4 kg m-2. A colheita foi realizada 180 dias após o plantio, em janeiro de 2011, sendo colhidas as plantas úteis (quatro plantas por parcela. Avaliou-se o rendimento de fitomassa fresca e seca. O delineamento experimental adotado foi o esquema fatorial 2x4, com quatro repetições (blocos. As médias obtidas foram submetidas à análise de variância seguida de teste de médias (Tukey. Com base nos resultados obtidos, pode-se concluir que a cama de aviário apresentou resultados mais satisfatórios quanto à produção de folhas de Ocimum selloi quando comparados aos demais tratamentos orgânicos, não se observando incremento nos resultados pela associação com o adubo verde Crotalaria juncea.The aim of this work was to avail the effect of different manures in association or nor with green manure on yield of leaves of Ocimum selloi Benth. The assay was accomplished on experimental area of Embrapa Environmental (Jaguariúna district, at two different spaces (with or without green manure Crotalaria juncea. The treatments used were T1 - witness (no manure, T2 - chicken manure (5 kg m-2, T3 - commercial product Fishfértil - 5 mL m-2 and T4 - composting (4 kg m-2. The cut was realized on 180 days after the planting (january - 2011, and were collected four plants/ plot. The yield of dried and fresh Ocimum

  5. Phytochemical evaluation and GC-MS analysis of Hyptis verticillata ...

    African Journals Online (AJOL)

    https://doi.org/10.2307/3434847. Gairola S, Shariff NM, Bhatt A, Kala CP. 2010. Influence of climate change on production of secondary chemicals in high altitude medicinal plants: Issues needs immediate attention. J. Med. Plants Res., 4(18): 1825-1829. Griffiths DW, Thomas T A. 1981. Phytate and total phosphorus content ...

  6. Levantamento etnobotânico de plantas popularmente utilizadas como antiúlceras e antiinflamatórias pela comunidade de Pirizal, Nossa Senhora do Livramento-MT, Brasil

    Directory of Open Access Journals (Sweden)

    Neyres Zínia Taveira de Jesus

    Full Text Available O trabalho teve por objetivo proceder o levantamento etnobotânico das espécies vegetais utilizadas popularmente no Distrito de Pirizal - MT, no pantanal mato-grossense, como antiúlceras e antiinflamatórias. A entrevista aberta foi realizada através da aplicação de um roteiro base a 38 informantes adultos, na faixa etária de 25 a 75 anos. Indagou-se o nome popular das plantas, partes utilizadas, preparados e vias de administração, e realizou-se a revisão bibliográfica das plantas mais citadas no estudo, utilizando-se as bases de dados convencionais. Foram citadas 49 espécies pertencentes a 47 gêneros e 32 famílias, destacando-se a família Fabaceae. As plantas mais citadas simultaneamente como antiúlceras e antiinflamatórias foram Lafoensia pacari St. Hil. (9,2%, Hyptis crenata Pohl (8,8%, Hyptis suaveolens (L. Poit (6,7%, Stachytarpheta cayenensis (L.C.Rich Vahl (5,8%, Waltheria indica L. (5%, Strychnos pseudoquina St. Hil. (4,2% e Vatairea macrocarpa (Benth. Ducke (3,3%. A parte da planta mais citada foi a folha (57,1%, a via de administração mais utilizada no tratamento das úlceras gástricas foi a oral (100%, com preferência para os chás (75%, enquanto nas inflamações foram os banhos tópicos (60%.. A revisão bibliográfica apontou a necessidade de aprofundar os estudos químico-farmacológicos para Vatairea macrocarpa (Benth. Ducke e Hyptis crenata Pohl.

  7. Desenvolvimento vegetativo e produção de óleo essencial de patchouli (Pogostemon cablin (Blanco Benth. após a aplicação de ácido giberélico e extrato de alga marinha

    Directory of Open Access Journals (Sweden)

    R.C. Storck

    2013-01-01

    Full Text Available Pogostemon cablin (Blanco Benth., comumente conhecido como patchouli, é uma espécie aromática utilizada pela indústria de perfumes devido a fragrância amadeirada e propriedade fixadora do óleo essencial. Fatores que afetam o teor dos constituintes do óleo essencial devem ser avaliados visando obter matéria prima de melhor qualidade. O experimento foi conduzido em casa de vegetação com delineamento inteiramente casualizado em esquema fatorial 2x3 e 4 repetições, sendo dois níveis de giberelina (0 e 200 mg L-1 e três níveis de extrato de alga marinha (0, 15 e 30 mg L-1. A aplicação dos reguladores vegetais foi realizada 30 dias após o plantio das mudas, e a avaliação do experimento deu-se 45 dias após a aplicação dos tratamentos. Não houve diferença significativa para a altura das plantas, a concentração de extrato de alga marinha de 15 mg L-1 promoveu aumento no número de folhas em relação à testemunha e a concentração de 30 mg L-1 promoveu decréscimo. Em relação à área foliar e ao número de folhas houve relação inversa para os níveis de extrato de alga marinha, onde o tratamento com maior concentração do regulador resultou em aumento da área foliar e redução do número de folhas. O teor de óleo essencial foi superior após a aplicação de 15 mg L-1 de extrato alga marinha isoladamente ou combinado com GA3. A produtividade do óleo essencial também aumentou com a aplicação 15 mg L-1 de extrato de alga marinha e quando utilizado somente GA3. A utilização de extrato de alga marinha aumentou a concentração de patchoulol no óleo essencial.

  8. 234 Fruiting Efficiency of Pentaclethra Macraphylla Benth: A Case ...

    African Journals Online (AJOL)

    Nekky Umera

    One of the major problems of P. macrophylla is the threat of extinction mainly due to .... evolution of mast fruiting in the aseasonal tropics. Am. Nat. 132-44-. 66. Bawa, K.S. and C.J. Webb (1984): Flower, fruit and seed abortions in tropical forest trees: implications for the evolution of maternal and paternal reproductive ...

  9. Hypoglycaemic effects of Parkia biglobosa (Jacq) Benth seed extract ...

    African Journals Online (AJOL)

    Mimosoideae), its chloroform, hexane, and mother liquor fractions were evaluated in glucose-loaded and alloxan-induced diabetic rats. The methanol extract of the seed exhibited a peak percentage decrease of 64% and 44.1% in blood glucose ...

  10. Bioassay-guided investigation of Lonchocarpus cyanescens benth ...

    African Journals Online (AJOL)

    diphenylpicrylhydrazyl (DPPH) free radical scavenging activity, ferric reducing power (FRAP), total phenol content and total flavonoid content using catechin as standard antioxidant. Bioguided column chromatographic separation was carried out and the resultant ...

  11. Contents of Aerial Parts of Salvia leriifolia Benth

    Directory of Open Access Journals (Sweden)

    Zahra Hosseinpoor Mohsen Abadi

    2016-06-01

    Full Text Available In the present study, we have reported the total phenolic content, total flavonoid content, antioxidant and antimicrobial activity of aerial parts of Salvia leriifolia extracts and fractions. Methanolic, n-hexane, chloroform, and ethyl acetate extracts were screened to analysis their antioxidant activities by four complementary test systems, namely DPPH free radical scavenging activity (RSA, total phenolic content (TPC, total flavonoid content (TFC, and ferrous ion cheating (FIC. In most cases the leaf extracts and ethyl acetate fraction had more activity. The methanolic extracts of leaf and flower showed considerable antimicrobial activity using disc diffusion method against Escherichia coli, Streptococcus pneumonia, Acinetobacter, Serratia, Pseudomonas aeruginosa. The extracts showed the highest activity against P. aeruginosa and K. pneumonia.

  12. PENGARUH PENGUSANGAN TERHADAP VIABILITAS BENIH WERU (Albizia procera Benth.

    Directory of Open Access Journals (Sweden)

    Eliya Suita

    2016-09-01

    Full Text Available Pengusangan dipercepat adalah pengujian yang menggunakan kondisi diperburuk dengan panas, oksigen, sinar matahari, getaran, dan lain-lain, untuk mempercepat proses penuaan benih. Hal ini digunakan untuk menentukan efek jangka panjang dari tingkat stres yang diharapkan dalam waktu yang lebih pendek, biasanya dilaksanakan di laboratorium dengan metode uji standar yang dikendalikan. Tujuan dari kegiatan ini adalah mengetahui pengaruh pengusangan terhadap viabilitas benih weru. Pengusangan dilakukan dengan cara menempatkan benih dalam bak plastik yang telah diisi air, kemudian dimasukkan ke dalam inkubator pada suhu 40°C selama jangka waktu tertentu sesuai dengan taraf pengusangan yang diberikan yaitu mulai dari pengusangan 120 jam sampai 880 jam. Pengusangan benih weru selama 880 jam sudah menurunkan daya berkecambah hingga daya berkecambah 69%. Benih weru dapat digolongkan kepada benih ortodoks sejati karena meskipun mendapatkan kondisi yang tidak menguntungkan viabilitasnya tetap tinggi.

  13. Antiproliferative activity of spinasterol isolated of Stegnosperma halimifolium (Benth, 1844

    Directory of Open Access Journals (Sweden)

    Salvador Enrique Meneses-Sagrero

    2017-12-01

    Full Text Available Cancer is the major cause of death in the world, representing a significant public health problem. Plants have been shown as a great source of secondary metabolites with anticancer activity. The aim of this work was evaluated the antiproliferative activity of the methanolic extracts, chemical fractions and the compound spinasterol isolated of medicinal plant Stegnosperma halimifolium. The methanolic extracts of stem, leaf and stem/leaf was obtained by maceration. The methanolic extract of stem was purified by successive extractions with solvents as n-hexane, ethyl acetate and ethanol. The n-hexane fraction was separated by column chromatographic and monitored by thin layer chromatographic. The compound spinasterol was characterized by 1H NMR, 13C NMR and Mass Spectrometry. Methanolic extracts, chemical, chromatographic fractions and spinasterol was evaluated against RAW 264.7, M12.C3.F6, PC-3, LS-180, A549 and HeLa cancer cell lines by the standardized method MTT for determinate the antiproliferative activity. Methanolic extract of stem shown the better antiproliferative activity against the murine macrophage cancer cell line RAW 264.7. n-Hexane chemical fraction shown antiproliferative activity against human alveolar cancer cell line A549 and RAW 264.7. Was isolated and characterized a compound by NMR 1H and 13C, revealing the presence of sterol spinasterol. Spinasterol shown to have antiproliferative activity against cervical cancer cell line HeLa and RAW 264.7, indicating that spinasterol can be a responsible compound of antiproliferative activity found in the methanolic extract of Stegnosperma halimifolium.

  14. Free radical scavenging potential of Picrorhiza kurrooa Royle ex Benth.

    Science.gov (United States)

    Govindarajan, R; Vijayakumar, M; Rawat, A K S; Mehrotra, Shanta

    2003-08-01

    For assessing free radical scavenging potential of P. kurrooa, the antioxidant activity of P. kurrooa extract was studied by lipid peroxidation assay using rat liver homogenate. The extract (1 mg/ml) showed marked protection (up to 66.68%) against peroxidation of liver phospholipids. Besides, reduced glutathione showed very encouraging activity. The extract also exhibited significant scavenging activity. Thus augmenting the wide use of plant in the indigenous system of medicine, which may partly be due to antioxidant and free radical scavening activity of the extract.

  15. Alkaloid and other chemical constituents from Psychotria stachyoides Benth

    Energy Technology Data Exchange (ETDEWEB)

    Pimenta, Antonia T.A.; Uchoa, Daniel E.A.; Silveira, Edilberto R.; Lima, Mary Anne S. [Departamento de Quimica Organica e Inorganica, Universidade Federal do Ceara, Fortaleza, CE (Brazil); Braz-Filho, Raimundo, E-mail: mary@dqoi.ufc.br [Centro de Ciencias, Universidade Estadual do Norte Fluminense and Universidade Federal Rural do Rio de Janeiro, Campos dos Goytacazes-RJ (Brazil)

    2011-09-15

    The organic extracts of leaves and roots of Psychotria stachyoides provided the new glucoside monoterpenoid indole alkaloid N-demethylcorreantoside, besides bizantionoside B, a-amyrin, alizarine methyl-ether, rubiadine, scopoletin, barbinevic acid and a mixture of b-sitosterol and stigmasterol glucosides. The structural characterization of the isolates was established based on infrared spectroscopy (IR), mass spectrometry (MS) and, particularly, 1D and 2D nuclear magnetic resonance (NMR). (author)

  16. Salvia broussonetii Benth.: aroma profile and micromorphological analysis.

    Science.gov (United States)

    Najar, Basma; Pistelli, Luisa; Cervelli, Claudio; Fico, Gelsomina; Giuliani, Claudia

    2017-10-26

    The volatile profiles (VOC) and the essential oil (EO) composition from the aerial parts of Salvia broussonetii were analysed. Sesquiterpene hydrocarbons dominate the VOCs from leaves (95.7%) and flowers (67.6%), followed by monoterpene hydrocarbons (2.6 and 29.7%, respectively). The main common compounds are germacrene D, β-bourbonene, α-pinene, α-copaene and α-gurjunene, even if with divergent relative abundances. In the leaf EOs the sesquiterpenes prevail, even if not overwhelmingly (about 50.0%), followed by monoterpenes (23.0-35.0%) and by minor fractions of diterpene hydrocarbons and non-terpene derivates. The most abundant common compounds across the two sampling periods are α-pinene, β-pinene, isobornyl acetate, α-gurjenene, germacrene D and bifloratriene. A morphological characterisation of the trichomes responsible for the productivity in terpenes was also performed. Four different morphotypes were observed on the above ground organs of S. brussonetii: peltates and capitates of type II and III resulted the only producers of volatile substances.

  17. Assessment of Bridelia ferruginea benth for its therapeutic potential ...

    African Journals Online (AJOL)

    Background: Pregnancy induced diabetes also known as gestational diabetes develops during pregnancy. Pregnancy is an insulin resistant state that may induce impaired glucose tolerance and often gestational diabetes in susceptible women. Gestational diabetes causes serious problems to the mother and the baby.

  18. Micropropagation of Micromeria juliana (L. Benth. ex Rchb. (Lamiaceae

    Directory of Open Access Journals (Sweden)

    Tošić, S.

    2015-09-01

    Full Text Available Micromeria juliana belongs to family Lamiaceae, whose representatives are characterized by a significant level of essential oils and antioxidant components. Several species of genus Micromeria contain biologically active substances and are therefore used in folk medicine, food industry and cosmetic industry. Methods of their tissue culture may provide rapid mass multiplication of plants for various purposes, including research on production, accumulation and metabolism of important secondary metabolites. The goal of this paper was to determine the protocol for regeneration of M. juliana plants through use of nodal explants on nutritive substrate with various growth regulators. The greatest number of axillary buds was formed in explants grown on MS nutritive medium with 3 μM benzyladenine (BA and 0.57 μM indole-3-acetic acid (IAA. The explants grown at the medium without any growth regulators and the medium with auxin have shown spontaneous root formation.

  19. Initiation and growth of shoots of Gongronema latifolia Benth stem ...

    African Journals Online (AJOL)

    There was significant reduction in number of days to shoot initiation and growth in sawdust medium in the wet season. Sawdust and soil gave a better performance of the cuttings in opening of apical buds, initiation of shoots, percentage of rooted cuttings, number of vines, vine length and number of opposite leaves on vines ...

  20. 234 Fruiting Efficiency of Pentaclethra Macraphylla Benth: A Case ...

    African Journals Online (AJOL)

    Nekky Umera

    inflorescence as a percentage of the pooled mean number of flowers produced per inflorescence. P.macrophylla had regular fruiting pattern in both locations with no ... commercial value especially in the southeastern part of Nigeria. Its seed is an essential component of the popular “African salad” served in homes, wedding ...

  1. Germinação de sementes de Enterolobium schomburgkii (Benth. Benth. submetidas a estresse salino e aplicação de poliamina Enterolobium schomburgkii (Benth. Benth. seed germination under saline stress and polyamine application

    Directory of Open Access Journals (Sweden)

    L.F. Braga

    2009-01-01

    Full Text Available Objetiva-se com este trabalho avaliar o efeito do estresse salino com NaCl e CaCl2 nos potenciais osmóticos 0 (controle; -0,2; -0,3 e -0,4 MPa com e sem aplicação da poliamina putrescina nas concentrações de 5 mM e 10 mM. As sementes de E. schomburgkii foram previamente escarificadas com lixa, no lado oposto à radícula, tratadas com fungicidas e colocadas para germinar nos diferentes tratamentos, à temperatura de 30ºC, sendo utilizadas quatro repetições de 25 sementes. Foi utilizado o delineamento inteiramente casualizado, em esquema fatorial 2 x 4 (agentes salinos x potenciais. No segundo experimento realizado após 11 meses, foram adicionadas as concentrações de 5 e 10 mM de putrescina. Foi utilizado o delineamento inteiramente casualizado, disposto em esquema fatorial 2 x 4 x 2 (agentes salinos x potenciais osmóticos x concentrações de putrescina. As sementes foram avaliadas quanto a porcentagem de germinação e índice de velocidade de germinação (IVG. Menor porcentagem de germinação e IVG foram observadas à medida que os potenciais se tornam mais negativos quando as sementes foram submetidas ao estresse salino com CaCl2. A aplicação de putrescina promoveu aumento na porcentagem de germinação e IVG sob os potenciais mais negativos, com maiores valores na concentração de 5 mM.The aim of this work was to evaluate the effects of salt stress by using NaCl and CaCl2 at the following osmotic potentials: 0 (control, -0.2, -0.3, and -0.4 MPa with and without putrescine application at 5 mM and 10 mM. E. Schomburgkii seeds were previously scarified with sandpaper in the region opposite to the radicle, treated with fungicides and allowed to germinate in the different treatments at 30ºC. The experimental design was completely randomized, in a 2x4 (osmotic potentials x saline agents factorial arrangement, with four replicates of 25 seeds each. After 11 months, a second experiment was carried out, in which putrescine concentrations of 5 and 10 mM were added and the experimental design was completely randomized, in a 2x4x2 (osmotic potentials x saline agents x putrescine concentrations factorial arrangement. Germination percentage and velocity index (GVI were evaluated. Germination percentage and GVI increased with lower potentials in seeds subjected to salt stress with CaCl2. Putrescine application (5mM increased germination percentage and GVI at the most negative potentials.

  2. Diuretic activity of the leaves of Coleus aromaticus Benth.

    Science.gov (United States)

    Choudhary, G P

    2009-07-01

    The aqueous and ethanolic extract of leaves of Coleus aromaticus was evaluated for diuretic activity. Both extracts were evaluated by determination of urine volume and electrolyte concentration in albino rats. Results revealed that both the aqueous and ethanolic extract at dose 500mgl kg showed significant diuretic activity by increasing the total volume of urine and concentration electrolyte. Furosamide (10 mg/kg) was used as reference drug while normal saline (0.9%) solution was used as control.

  3. Genetic Transformation of Coleus blumei Benth. Using Agrobacterium

    Directory of Open Access Journals (Sweden)

    Nataša Bauer

    2002-01-01

    Full Text Available Efficiency in transformation of three hybrids of Coleus blumei using wild and mutant strains of Agrobacterium sp. was evaluated. Successful transformation depended on specific bacterial strain-plant genotype combination and co-cultivation treatment. The wild type B6S3 of the bacterial strains employed gave the maximum efficiency. Strains C58C1(pArA4abc, GV3101(pGV2215, 8196 and A281 were also effective. Among several co-cultivation conditions tested, the most efficient treatment was as follows: excised leaf explants were incubated immeadiately in bacterial suspension for 5 min and were co-cultured with Nicotiana tabacum crown gall callus for two days. Plant hormone autotrophy, PCR analyses and hybridization analysis confirmed genetic transformation of the cultures. Sixteen different transgenic callus and cell cultures were maintained for more than four years in the absence of exogenous growth regulators and antibiotics without deterioration in the growth rate.

  4. Integrated control of Striga hermonthica (Del.) Benth. in Burkina ...

    African Journals Online (AJOL)

    A two-year field study investigated integrated management of Striga hermonthica on sorghum through host crop resistance, biocontrol (Fusarium oxysporum isolate 34-FO), and fertilizer application. The experiment was conducted in a field with natural Striga infestation at Kouaré Research Station in Fada N'Gourma, Burkina ...

  5. (Jacq) Benth (African Locust Bean) on Hypertension in Bogou and

    African Journals Online (AJOL)

    Erah

    2010-09-09

    Sep 9, 2010 ... Minerals. Plasma potassium, sodium, chloride, magnesium and calcium were determined using commercial diagnostic kits (Cypress. Diagnostics Laboratory) on an automated spectrophotometer (Clima Plus, Ral.SA), following the kit manufacturers' guide in each case. For potassium, the absorbance was.

  6. STUDIES ON THE EFFICACY OF BRIDELIA FERRUGINEA BENTH ...

    African Journals Online (AJOL)

    3Department of Medical Microbiology and Parasitology College of Medicine,. University of Ilorin, Nigeria ... The dried pieces were then pulverized using the laboratory mill (Christy and Morris Limited, .... The interest shown in the second half of the nineteenth century in chemical methods for treating wastewater is being ...

  7. Bioassay-guided investigation of Lonchocarpus cyanescens benth ...

    African Journals Online (AJOL)

    SAM

    2014-05-28

    May 28, 2014 ... health related benefits (Mali et al., 2004; Chaudhary et al., 2004). Recently, there has been growing interest in natural antioxidants of plant origin ..... antioxidant action of caffeine: mechanistic considerations, Ind. J. Exp. Biol. 41:267-269. Evans P, Halliwall B (1999). “Free radicals and hearing” Ann N. Y..

  8. Two New Phenolic Compounds from Schizonepeta tenuifolia (Benth. Briq

    Directory of Open Access Journals (Sweden)

    Xu-Hua Huang

    2016-03-01

    Full Text Available Two new phenolic compounds, Schitenoside A (1 and Schitenoside B (2, have been isolated together with six known compounds: 3,4-dihydroxyphenethyl alcohol-4-O-β-D-glucopyranoside (3, 2-(3-hydroxy-4-methoxyphenyl ethanol 1-O-α-L-rhamnopyranosyl-(1→2-β-D-glucopyranoside (4, benzyl 7-O-β-D-xylopyranosyl-(1→6-β-D-glucopyranoside (5, 2-hydroxybenzoic acid (6, m-hydroxybenzoic acid (7 and trans-caffeic acid (8, from the Schizonepeta tenuifolia. Their structures were elucidated by spectroscopic analysis. Compounds (3-7 were isolated from Schizonepeta genus for the first time. Compounds 1 and 2 showed a week antibacterial activity against four test strains, involving both Gram-positive and Gram-negative bacteria .

  9. Management of Striga Hermonthica (Del.) Benth in Upland Rice ...

    African Journals Online (AJOL)

    The effects of different rates of Nitrogen (0, 30, 60, 90 and 120 kg N/ha) on the reaction of five (FARO 46, FARO 11, FARO 45, FARO 48 and FARO 38) and seven (FARO 46, FARO 11, FARO 45, FARO 48, FARO 38, FARO 40 and WAB 56-50) varieties of upland rice were studied in field trials conducted in both the dry and ...

  10. Antioxidant and Antimicrobial Activities of Pogostemon cablin (Blanco Benth.

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    Bhanuz Dechayont

    2017-01-01

    Full Text Available Pogostemon cablin (Lamiaceae has been widely used in traditional medicine. In this study, the antioxidant and antimicrobial activities of leaves from P. cablin extracts were investigated. The water extracts had the highest total phenolic content 116.88±0.48 mg gallic acid equivalent/g of dry plant extract. Nevertheless, high levels of total flavonoid content were found in ethanolic extracts 280.12±2.04 mg quercetin equivalent/g of dry plant extract. The highest antioxidant activities were found for the ethanolic extract (IC50=18±0.90, 20±0.24 μg/mL by DPPH and ABTS scavenging assays, respectively. Both extracts showed moderate inhibition of superoxide inhibition (O2∙- and nitric oxide (NO production in concentration-dependent manner. Antibacterial activity was calculated by disk diffusion, minimum inhibitory concentration (MIC, and minimum bacterial concentration (MBC. The ethanolic extract had the greatest activity against methicillin resistant Staphylococcus aureus, methicillin sensitive S. aureus, and Streptococcus pyogenes with zone diameters of 11.67±1.53, 10.33±2.52, and 10.33±1.15 mm, respectively. The corresponding MIC and MBCs were 5, 0.625, and 0.039 mg/mL. P. cablin extracts contain antioxidant and antibacterial properties that should be exploited for possible clinical application.

  11. Morphometric Studies of Clausena anisata (Willd.) Hook. f. ex. Benth ...

    African Journals Online (AJOL)

    Numerical taxonomic study was conducted on five populations of C. anisata in the coastal savanna zone of Ghana to determine their patterns of taxonomic variation and identify diagnostic characters among the populations studied. The results showed that the five populations of C. anisata exhibited complex patterns of ...

  12. Assessment of Bridelia ferruginea benth for its therapeutic potential ...

    African Journals Online (AJOL)

    McRoy

    administration volume of 1.0ml/100g bw. Results: Oral glucose tolerance test showed that pregnancy induced glucose intolerance in the rats. However, B. ferruginea caused a reduction in glycaemic response to glucose challenge and an increased glucose tolerance in rats that had pregnancy-induced glucose intolerance.

  13. Albizzia lebbeck Benth. (Hindi: Siris) of Leguminosae is a large ...

    Indian Academy of Sciences (India)

    ... the entire crown of the tree covered with previous season's dry pods). The tender branchlets and leaves are used as fodder. The heartwood is dark brown, tough and durable and is used in making cart wheels and agricultural implements. The bark and seeds are astringent and are given for treating piles and diarrhoea.

  14. Response of African oil bean ( Pentaclethra Macrophylla Benth ...

    African Journals Online (AJOL)

    This study evaluated the response (seedling emergence) of Pentaclethra macrophylla seeds sown in soils contaminated with spent lubricating oil using 0, 1, 2, 4 and 8% w/w in Asaba, Delta State in 2009. The results showed that 100% of the seeds sown in the control germinated. Significant reductions (P ≥ 0.05) were ...

  15. Antimicrobial activity of some medicinal plants from the cerrado of the central-western region of Brazil

    Directory of Open Access Journals (Sweden)

    Ivana Maria Póvoa Violante

    2012-12-01

    Full Text Available Ethanol extracts from six selected species from the Cerrado of the Central-Western region of Brazil, which are used in traditional medicine for the treatment of infectious diseases and other medical conditions, namely Erythroxylum suberosum St. Hil. (Erythroxylaceae, Hyptis crenata Pohl. ex Benth. (Lamiaceae, Roupala brasiliensis Klotz. (Proteaceae, Simarouba versicolor St. Hil. (Simaroubaceae, Guazuma ulmifolia Lam. (Sterculiaceae and Protium heptaphyllum (Aubl. March. (Burseraceae, as well as fractions resulting from partition of these crude extracts, were screened in vitro for their antifungal and antibacterial properties. The antimicrobial activities were assessed by the broth microdilution assay against six control fungal strains, Candida albicans, C. glabrata, C. krusei, C. parapsilosis, C. tropicalis and Cryptococcus neoformans, and five control Gram-positive and negative bacterial strains, Escherichia coli, Enterococcus faecalis, Klebsiella pneumoniae, Pseudomonas aeruginosa and Staphylococcus aureus. Toxicity of the extracts and fractions against Artemia salina was also evaluated in this work. All plants investigated showed antimicrobial properties against at least one microorganism and two species were also significantly toxic to brine shrimp larvae. The results tend to support the traditional use of these plants for the treatment of respiratory and gastrointestinal disorders and/or skin diseases, opening the possibility of finding new antimicrobial agents from these natural sources.Among the species investigated, Hyptis crenata, Erythroxylum suberosum and Roupala brasiliensis were considered the most promising candidates for developing of future bioactivity-guided phytochemical investigations.

  16. Antimicrobial activity of some medicinal plants from the cerrado of the centralwestern region of Brazil

    Science.gov (United States)

    Violante, Ivana Maria Póvoa; Hamerski, Lidilhone; Garcez, Walmir Silva; Batista, Ana Lucia; Chang, Marilene Rodrigues; Pott, Vali Joana; Garcez, Fernanda Rodrigues

    2012-01-01

    Ethanol extracts from six selected species from the Cerrado of the Central-Western region of Brazil, which are used in traditional medicine for the treatment of infectious diseases and other medical conditions, namely Erythroxylum suberosum St. Hil. (Erythroxylaceae), Hyptis crenata Pohl. ex Benth. (Lamiaceae), Roupala brasiliensis Klotz. (Proteaceae), Simarouba versicolor St. Hil. (Simaroubaceae), Guazuma ulmifolia Lam. (Sterculiaceae) and Protium heptaphyllum (Aubl.) March. (Burseraceae), as well as fractions resulting from partition of these crude extracts, were screened in vitro for their antifungal and antibacterial properties. The antimicrobial activities were assessed by the broth microdilution assay against six control fungal strains, Candida albicans, C. glabrata, C. krusei, C. parapsilosis, C. tropicalis and Cryptococcus neoformans, and five control Gram-positive and negative bacterial strains, Escherichia coli, Enterococcus faecalis, Klebsiella pneumoniae, Pseudomonas aeruginosa and Staphylococcus aureus. Toxicity of the extracts and fractions against Artemia salina was also evaluated in this work. All plants investigated showed antimicrobial properties against at least one microorganism and two species were also significantly toxic to brine shrimp larvae. The results tend to support the traditional use of these plants for the treatment of respiratory and gastrointestinal disorders and/or skin diseases, opening the possibility of finding new antimicrobial agents from these natural sources. Among the species investigated, Hyptis crenata, Erythroxylum suberosum and Roupala brasiliensis were considered the most promising candidates for developing of future bioactivity-guided phytochemical investigations. PMID:24031956

  17. Effects of Ethanolic Extract of Hyptis Suavoelens on the Food, Water ...

    African Journals Online (AJOL)

    A total of two hundred and fifteen animals, one hundred and fifteen Wistar rats and one hundred albino mice were used for the various aspect of the study. The LD50 of the extract was found to be 1264.91 + 0.51mg/kg (I.P) in mice and rats. Weight loss was observed in male and female rats to be significant (P < 0.05).

  18. HOJAS DE CHAN (Hyptis suaveolens PARA EL CONTROL DE Sitophilus zeamais Y Zabrotes subfasciatus

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    Modesto Armando G\\u00F3mez-Peralta

    2009-01-01

    Full Text Available Con el obje­tivo de evaluar el efecto de la hoja de chan sobre Sitophilus zeamais y Zabrotes subfasciatus en granos almacenados de maíz y fri­jol respectivamente, se estableció un ensayo en condiciones controladas en el año 2007, entre los meses de mayo a setiembre, en la ciudad de León, Nicaragua, a 129 msnm y una temperatura promedio de 29 °C. Se emplearon semillas de frijol de la variedad DOR 364 y semillas de maíz de la variedad NB - 6. Las hojas de chan del estrato medio de las plantas hacia el estrato superior y de tamaño similar, se colectaron antes de la floración se secaron por tres días en un secador solar y luego fueron pulverizadas. Las unidades experimentales consistieron de frascos de plástico de 172,2 g de capacidad, y dimensiones de 7,5 cm de diámetro por 8 cm de alto, a los que se adicionaron 100 g de maíz o 100 g de frijol. Los tratamientos consistieron en la adición del polvo de hoja seca de chan entre las semillas en seis dosis: 0, 5, 10, 15, 20 y 25 gramos por 100 de semilla. Luego se adicionaron 20 adultos seleccionados al azar y sin sexar. Las variables medidas en frijol y maíz fueron: número de insectos muertos; número de granos picados; número de adultos mergidos de los granos y peso del los granos. El polvo de las hojas de chan sólo tuvo efecto en la mortalidad de S. zeamays.

  19. Evaluation de l\\'activité insecticide de deux plantes Hyptis ...

    African Journals Online (AJOL)

    et de Khaya senegalensis (A. Juss), issus de matériels végétaux conservés pendant un an, trois mois ou un jour à 5°C. Les ravageurs ciblés sont Aphis craccivora Koch, Megalurothrips sjostedti Trybom et les bruches. Au champ, Callosobruchus maculatus Fabricius et son parasitoïde Uscana lariophaga Steffan sont visés.

  20. Effet de la monoculture des poacees sur le controle de Hyptis ...

    African Journals Online (AJOL)

    These seeds in soil maintained their viability for about two years. Ploughing and hand weeding induced H. suaveolens spontaneous regeneration. During two consecutive years, the decrease in germination rate of H. suaveolens in Poaceae plots, ranged from 44.7 to 73.6% versus 1.6% in control plot. Germination rate and ...

  1. In vitro toxicity of extracts from Hyptis suaveolens (L.) poit on eggs ...

    African Journals Online (AJOL)

    The essential oil (EO) compared well with the standard carbofuran (CBFN) at 65.58% and 66.06% mortality respectively, while the dichloromethane and aqueous extracts were not as effective. Mortality increased with increase in exposure time. There was total egg hatch inhibition with the essential oil and carbofuran, all the ...

  2. Sesquiterpenes and other constituents from leaves of Pterodon pubescens Benth (Leguminosae); Sesquiterpenos e outros constituintes das folhas de Pterodon pubescens Benth (Leguminosae)

    Energy Technology Data Exchange (ETDEWEB)

    Miranda, Mayker Lazaro Dantas; Garcez, Fernanda Rodrigues; Garcez, Walmir Silva, E-mail: walmir.garcez@ufms.br [Universidade Federal de Mato Grosso do Sul (UFMS), Campo Grande, MS (Brazil). Instituto de Quimica; Abot, Alfredo Raul [Universidade Estadual de Mato Grosso do Sul (UEMS), Aquidauana, MS (Brazil)

    2014-05-15

    In addition to β-sitosterol, stigmasterol, phaeophitin A, luteolin, kaempferol, quercetin, (+)-catechin, quercetin-3-O-α-L-rhamnopyranoside, rutin, and p-hydroxy-benzoic acid, six known sesquiterpenes, namely (rel)-2β,6β-epoxy-5β-hydroxy-isodaucane, oplopanone, 1β,6α-dihydroxy-4(15)-eudesmene, caryophyllene oxide, α-cadinol, and spathulenol, were isolated from the leaves of Pterodon pubescens (Leguminosae) growing in the Cerrado of Mato Grosso do Sul, Brazil. The (rel)-2β,6β-epoxy-5β-hydroxy-isodaucane corresponds to the correct structure of homalomenol D. The sesquiterpene oplopanone, which bears a modified cadinane skeleton, is being reported for the first time in this genus. The structures of the compounds were determined on the basis of spectral data (MS, IR, and NMR-1D and 2D) and subsequent comparison with data reported in the literature. (author)

  3. Una nueva especie de Centrosema (DC. Benth. (Leguminosae: Papilionoideae del Orinoco Una nueva especie de Centrosema (DC. Benth. (Leguminosae: Papilionoideae del Orinoco

    Directory of Open Access Journals (Sweden)

    Schultze Kraft Rainer

    1990-09-01

    Full Text Available A new species of Centrosema is described and named C. tetragonolobum. It occurs in the region of the Orinoco river between latitudes 4° and 6° N in the Comisaría del Vichada, Colombia, and the Territorio Federal Amazonas, Venezuela. Characteristic features of the species are winged pods, chartaceous to coriaceous leaves, and an erect-climbing growth habit. Una nueva especie de Centrosema es descrita como C. tetragonolobum. Su distribución está limitada a la región del río Orinoco entre latitudes 4° y 6° N en la Comisaría del Vichada, Colombia, y el Territorio Federal Amazonas, Venezuela. Son características de la especie las legumbres aladas, hojas cartáceas a coriáceas, y un hábito de crecimiento erecto-trepador.

  4. Caracterização anatômica das folhas de Cunila microcephala Benth. (Lamiaceae Anatomic characterization of Cunila microcephala Benth. (Lamiaceae leaves

    Directory of Open Access Journals (Sweden)

    Maria da Graça Teixeira de Toledo

    2004-12-01

    Full Text Available No presente trabalho fez-se a análise da organização estrutural das folhas de Cunila microcephala em microscopia fotônica e microscopia eletrônica de varredura. Seguiram-se as técnicas convencionais no preparo do material para obtenção das lâminas semipermanentes. Para a preparação de lâminas permanentes utilizou-se a técnica de inclusão em glicol metacrilato (GMA. A organização estrutural das folhas desta espécie revela: estômatos em ambas as faces com predominância na face abaxial (folha anfi-hipoestomática. Os estômatos da face adaxial são do tipo diacítico. As paredes anticlinais das células epidérmicas da face adaxial são sinuosas e apresentam espessamentos irregulares. As epidermes de ambas as faces são uniestratificadas. Tricomas tectores unisseriados e tricomas glandulares do tipo capitado unicelular e pluricelular se fazem presentes em ambas as faces. O mesofilo é heterogêneo dorsiventral. Idioblastos contendo cristais de inulina se fazem presentes em toda a extensão do limbo. A nervura principal em secção transversal, na porção mediana da lâmina foliar revela uma organização bastante simples, pouco saliente, na qual o parênquina clorofiliano paliçádico apresenta solução de continuidade. Os feixes vasculares são colaterais.This work has focused on the structural organization analysis of Cunila microcephala leaves in photonic microscopic and electronic scanning. Conventional techniques have been used to prepare the material for obtaining semi-permanent plates. For the preparation of permanent plates, the immersion into glycol methacrylate (GMA has been performed. The structural organization of leaves belonging to this species reveals stomata in both faces with predominance in the abaxial face (amphihypostomatic leaf. The adaxial face stomata belongs to the diacytic type. The anticlinal walls of the epidermis cells of the adaxial face are sinuous and present irregular thickening. The epidermis of both faces is single layered. Uniserial tectorial trichomes and single celled and multicelled capitate glandular trichomes are present in both faces. The mesophyll is heterogeneous dorsi-ventral. Idioblasts containing inulin crystals are present throughout the foliar plate. The central veins in cross-section, in the medium portion of the foliar plate, have shown a very simple organization, not an outstanding one, where the chlorophyllian palisade parenchyma presents a solution of continuity. The vascular fagots are collateral.

  5. OSMODESHIDRATACIÓN DE MORA DE CASTILLA (Rubus glaucus Benth CON TRES AGENTES EDULCORANTES OSMODEHYDRATION OF BLACKBERRY (Rubus glaucus Benth WITH THREE SWEETENING AGENTS

    Directory of Open Access Journals (Sweden)

    Diana Patricia Giraldo Bedoya

    2004-06-01

    Full Text Available La investigación sobre la deshidratación osmótica de mora de castilla con tres jarabes diferentes, sacarosa (js, sacarosa invertida (jsi y miel de caña (jmc, en iguales condiciones iniciales de concentración, 70 grados Brix, temperatura promedio de 20°C, y humedad relativa de 65% desarrollada en el Laboratorio de Frutas y Hortalizas de la Universidad Nacional de Colombia, Sede Medellín, permitió conocer que el jarabe de miel de caña presenta mayor poder osmótico (69,2% que los jarabes de sacarosa invertida (54,5% y sacarosa (50%, medido a partir del porcentaje de pérdida de peso de la mora. Con el proceso de estabilización del producto secado por convección forzada con aire caliente a 1,5 ms-1 de velocidad y 55°C de temperatura, durante 24 horas, logrando disminuir la humedad de los tres productos hasta 27,3%hbh, 30,8%hbh y 25,9%hbh para los jarabes de sacarosa, sacarosa invertida y miel de caña, respectivamente y mejorar las condiciones de empaque y almacenamiento, haciendo más estable el producto al ataque microbiano. Las pruebas preliminares de conservación se efectuaron en envases de vidrio de 250g durante 15 días. Se realizó además una prueba sensorial de ordenación con 10 jueces entrenados para la evaluación de las moras deshidratadas osmóticamente antes y después de la prueba de empaque y almacenamiento y como resultado se obtuvo que antes de la prueba de empaque y almacenamiento, el producto de mayor aceptación fue el correspondiente a las moras osmodeshidratadas en jarabe de sacarosa invertida y luego del empaque, la mayor aceptación fue para las moras osmodeshidratadas en jarabe de sacarosa.Studies of osmotic dehydration of blackberry with three different syrups, sucrose (ss, reverse sucrose (rss, and cane syrup (cs with identical initial concentration conditions, 70°Brix, mean temperature of 20°C, and relative humidity of 65% were undertaken in the Fruit and Vegetable Laboratory of the Universidad Nacional de Colombia, Sede Medellín, showing that the cane syrup presented greater osmotic potential (69,2% compared to the reverse sucrose (54,5% and sucrose (50%, measured as the percentage weight loss of the blackberry samples. With the product dehydration process of drying with forced convection hot air at 1,5 ms-1 velocity and 55°C during 24 hours, it was possible to reduce the humidity of the three products to 27,3% hbh, 30,8% hbh and 25,9 % hbh for syrups of sucrose, reverse sucrose and cane syrup, respectively and improve the packaging and storage conditions, making the product more stable against microbial attacks. An ordenation sensorial test also was conducted with 10 judges trained for evaluation of osmotically dehydrated blackberries before and after the packing and storage process, the most accepted product was the one corresponding to the blackberries osmodehydrated in inverted sucrose syrup and after packaging the most accepted was blackberries osmodehydrated in sucrose syrup.

  6. Anatomia foliar de Rudgea decipiens Müll. Arg. e R. macrophylla Benth. (Rubiaceae Leaf anatomy of Rudgea decipiens Müll. Arg. and R. macrophylla Benth. [Rubiaceae

    Directory of Open Access Journals (Sweden)

    André Mantovani

    1995-12-01

    Full Text Available São apresentados dados sobre a anatomia das folhas de Rudgea decipiens e R. macrophylla (Rubiaceae. Ambas ocorrem na Floresta de Tijuca (Mata Atlântica, Rio de Janeiro. As folhas são hipostomáticas, com estômatos do tipo paralelocítico, dotadas de mesofilo dorsiventral, feixes vasculares do tipo colateral, papilas na epiderme abaxial e cutícula estriada. Em R. macrophylla, destaca-se a ocorrência de estômatos peculiares.Anatomical characteristics of Rudgea decipiens and R. macrophylla leaves were examined. The two species grow in Floresta da Tijuca (Mata Atlântica, Rio de Janeiro municipality. The leaves are hipostomatics, with parallelocytic stomata, dorsiventral mesophyll, colateral vascular bundles, papillae on the lower surface and striate cuticle. The stomata show peculiar aspects in R. macrophylla.

  7. Chemical constituents of Hyptidendron canum (Pohl ex Benth.) R. Harley (Lamiaceae); Constituintes quimicos de Hyptidendron canum (Pohl ex Benth.) R. Harley (Lamiaceae)

    Energy Technology Data Exchange (ETDEWEB)

    Lemes, Geralda de Fatima; Ferri, Pedro Henrique, E-mail: geralda@quimica.ufg.b [Universidade Federal de Goias (UFG), Goiania, GO (Brazil). Inst. de Quimica; Lopes, Marcia Nasser [Universidade Estadual Paulista (IQ/UNESP), Araraquara, SP (Brazil). Inst. de Quimica

    2011-07-01

    Chemical investigation of Hyptidendron canum stems resulted in the isolation of betulinic, ursolic and euscaphic acids. From the leaves were isolated 3{beta}-O-{beta}-galactopiranosilsitosterol, ursolic aldehyde, and mixtures of maslinic acid and 2{alpha}-hydroxy ursolic acid, {alpha} and {beta}-amyrin, uvaol and erythrodiol, sitosterol and stigmasterol, spathulenol and globulol. Hexane and chloroform leave fractions as well as ursolic and betulinic acids showed antifungal activities against the yeast form of Paracoccidioides brasiliensis. (author)

  8. Potencial de rizobactérias no crescimento de mudas de sibipiruna (caesalpinia peltophoroides BENTH Epotential of rhizobacterias for the growth of seedlings of sibipiruna (Caesalpinia peltophoroides BENTH

    Directory of Open Access Journals (Sweden)

    Jeane de Fátima Cunha

    2013-04-01

    Full Text Available Este trabalho objetivou avaliar o potencial de isolados de rizobactérias na germinação de sementes e no crescimento de mudas de sibipiruna (Caesalpinia peltophoroides. Testaram-se os isolados pré-selecionados para eucalipto, Ca, FL2, MF2, MF4, RC3, R1, 3918, S1, S2 e CIIB. Para tanto, amostras de substrato à base de vermiculita e casca de arroz carbonizada (1:1 foram tratados com 5 mL de uma suspensão de cada isolado (OD540= 0,2 A/ tubete de 55 cc de capacidade, correspondendo a cerca de 10 ufc/mL. O delineamento experimental foi inteiramente casualizado (DIC com cinco repetições por tratamento, com 20 sementes cada. Aos 40 dias, avaliaram-se a porcentagem de germinação e a massa seca de raízes e da parte aérea. Verificou-se aumento significativo em matéria seca de raiz e da parte aérea para todos os isolados de rizobactérias testados, em relação à testemunha. Todos os isolados proporcionaram aumento significativo na germinação, à exceção do 3918 e CIIB que não diferiram da testemunha. Entre os isolados testados, quatro destacaram-se como os mais promissores (FL2, MF4, MF2 e CIIB. Os resultados obtidos mostram ganhos significativos na produção de mudas, sem nenhum ajuste no manejo ou na estrutura do viveiro. Além desse ganho direto, pode-se ter um melhor aproveitamento da estrutura física dos viveiros, ao se diminuir o tempo de formação das mudas, reduzindo-se o custo de produção.The objective of this work was to evaluate the potential of growth-promoting rhizobacteria for seed germination and biomass of the root system and aerial part of seedlings of sibipiruna ( Caesalpinia Peltophoroides: . Isolates pre-selected for eucalyptus were used, Ca, FL2, MF2, MF4, RC3, R1, 3918, S1, S2 and CIIB. Thus, samples of vermiculite-based substrate and carbonized rice hull (1:1 were treated with 5 mL of a suspension of each isolate (OD540= 0.2 A/ tubette of 55 cc capacity corresponding to around 10 ufc/mL. The experimental design was completely randomized (CRD with five repetitions per treatment, each constituted by 20 seeds. At 40 days, germination percentage and dry mass of the roots and the aerial parts were evaluated. A significant increase was observed in dry matter of the root and aerial part for all the rhizobacteria isolates tested, compared to the control. All the isolates provided significant germination increase, except 3918 and CIIB, which did not differ from the control. Among the tested isolates, four were the most promising (FL2, MF4, MF2 and CIIB. The results obtained show significant gains in seedling production, without any adjustment in nursery management or structure. In addition to this direct gain, it is possible to make a better use of the physical structure of the nurseries by reducing the time needed for seedling formation, reducing the production costs.

  9. Caracterização físico-química de méis produzidos no Estado do Piauí para diferentes floradas Physical and chemical characterization of honeys produced in the State of Piauí - Brazil

    Directory of Open Access Journals (Sweden)

    Claudécia L. da Silva

    2004-12-01

    Full Text Available Este trabalho foi realizado com o objetivo de se caracterizar físico-químicamente méis de Apis mellifera L. produzidos no Estado do Piauí, a partir de floradas típicas da região semi-árida do Nordeste brasileiro e, ao mesmo tempo, avaliar a qualidade desses produtos. Analisaram-se os méis produzidos a partir das floradas de marmeleiro (Croton sonderianus Müll. Arg., jitirana (Hyptis suaveolens, camaratuba (Cratylia mollis Mart. ex Benth, silvestre (Serjania glabrata e angico de bezerro (Piptadenia moniliformis, seguindo-se as metodologias recomendadas pelo Ministério da Agricultura e do Abastecimento, através de Instrução Normativa de outubro/2000. Foram realizadas as seguintes análises: umidade, hidroximetilfurfural (HMF, açúcares redutores, sacarose aparente, cinzas, acidez livre, sólidos insolúveis em água, atividade diastásica, ºBrix e pH. Os valores individuais de cada variável analisada, comparados com os padrões estabelecidos pelo Ministério da Agricultura e do Abastecimento, apresentaram resultados satisfatórios encontrando-se todos os méis analisados, dentro dos padrões exigidos na citada Instrução Normativa.The objective of this work was to evaluate the physical-chemical composition of honeys produced in the Piauí State, located in semi-arid region of Northeast Brazil. The honeys produced from 'marmeleiro' (Croton sonderianus Müll. Arg., 'jitirana' (Hyptis suaveolens, 'camaratuba' (Cratylia mollis Mart. ex Benth, 'silvestre' (Serjania glabrata and 'angico de bezerro' (Piptadenia moniliformis, were analysed following the methods recommended by the Brazilian Ministry of Agriculture and Normative Instruction of October 2000. the following analyses were performed: moisture, hydroxymethylfurfural (HMF, reducing sugars, apparent sucrose, ash, free acidity, insoluble solids in water, diastatic activity, ºBrix and pH. The individual values of each analyzed parameter were compared with the standards established

  10. Assessment of genetic diversity of a native population of Eplingiella fruticosa: a plant with therapeutic potential.

    Science.gov (United States)

    Silva, D C; Diniz, L E C; Blank, A F; Nizio, D A C; Pinto, J A O; Pereira, K L G; Arrigoni-Blank, M F

    2017-08-17

    Eplingiella fruticosa (Salzm. ex Benth.) Harley & J.F.B. Pastore, ex Hyptis fruticosa Salzm. ex Benth. is an aromatic and medicinal plant of the family Lamiaceae, found mainly in regions with intense anthropic activity. Information on the genetic diversity of this species is scarce. However, it can be assessed using molecular markers that identify the level of diversity among phenotypically identical individuals. The present study aimed to characterize the genetic diversity of a native population of E. fruticosa from the State of Sergipe using ISSR molecular markers. Samples of 100 plants were collected in 11 municipalities of the State of Sergipe and analyzed using eight ISSR primers, resulting in 72 informative bands. The cluster analysis obtained using the neighbor joining method resulted in three groups: Group I consisted of 50 plants, mainly from the municipalities of Areia Branca, Estância, Japaratuba, Moita Bonita, Pirambu, and Salgado; Group II was formed by 21 plants, with nine representatives from the municipality of Itaporanga d'Ajuda and 13 representatives from other municipalities; Group III was composed by 29 plants, being represented mainly by the municipalities of Malhada dos Bois and São Cristóvão. The smallest genetic distance occurred between plants EPF94 and EPF96 (0.250), and the greatest distance occurred between plants EPF50 and EPF96 (0.9778). The Shannon index had a mean value of 0.42, and diversity was considered moderate. Heterozygosity had a mean value of 0.267 and was considered low. Polymorphic information content (0.253) was considered moderately informative. Genetic diversity of E. fruticosa plants was intermediate, and the results of the present study can assist in the conservation and use of the genetic resources of this species.

  11. Juvenile Demography of Copaifera pubiflora Benth. (Leguminosae, Caesalpinioideae) near Parent Tree

    OpenAIRE

    NELSON, RAMIREZ; OMAIRA, HOKCHE; Universidad Central de Venezuela, Fac. de Ciencias, Centro Botanica Tropical

    1995-01-01

    The age structure and distribution of seeds, seedlings, and non-reproductive (juveniles) plants of Copaifera pubiflora relative to the distance from parent trees were studied in the Venezuelan central plains. Age structure differentiated between years when comparisons were made for plants in one-year interval classes. This difference was associated with the proportion of plants less than one year old and those eight years old. Age structure did not differ between years when comparisons were m...

  12. Crown-Stump Diameter Model for Parkia biglobosa Benth. Species in Makurdi, Benue State, Nigeria

    Directory of Open Access Journals (Sweden)

    O. Chukwu

    2017-07-01

    Full Text Available The crown of tree is the centre of physiological activity which gives an indication of the potential photosynthetic capacity on a tree. Though, its measurement remains a challenge in forest inventory task. The ability to predict crown diameter from stump diameter provides an effective technique of obtaining its estimate. This helps in detecting the excessive tree felling than actual requirements and wildlife suitability.The main objective of this study was to develop and test crown diameter prediction models for silvicultural management of naturally grown Parkia biglobosa within the University of Agriculture, Makurdi. Nine 100 m x 100 m temporary sample plots were established using simple random sampling method. Crown diameter and stump diameter were measured in all living P. biglobosa trees with stump diameter ≥10.0 cm. Least square method was used to convert the counted stumps into harvested crown dimension. Three linear and three non-linear models using stump diameter as the exploratory variable were developed and evaluated using the adjusted coefficient of determination (Adj.R2, standard error of estimate (SEE, prediction error sum of squares (PRESS and Akaike information criterion (AIC. The crown-stump diameter relationship was best described by the double logarithmic function with .The result showed that Crown diameter estimation was feasible even when the only information available is stump diameter.The resulting equation was tested for validation with independent data obtained from additional plots and was found to be desirable for estimating the crown diameter for Parkia biglobosa in Makurdi, Benue State, Nigeria.

  13. Antileishmanial activity of 12-methoxycarnosic acid from Salvia repens Burch. ex Benth. (Lamiaceae)

    CSIR Research Space (South Africa)

    Mokoka, TA

    2014-01-01

    Full Text Available In South Africa, Salvia repens is used traditionally to treat sores, stomach ache and diarrhoea. The high performance liquid chromatography (HPLC)-based activity profiling of S. repens whole plant extract showed an active abietane diterpene...

  14. Picrorhiza kurroa (Kutaki Royle ex Benth as a hepatoprotective agent--experimental & clinical studies.

    Directory of Open Access Journals (Sweden)

    Vaidya A

    1996-10-01

    Full Text Available Picrorhiza kurroa (Pk, a known hepatoprotective plant, was studied in experimental and clinical situtations. The standardization of active principles--Picroside 1 and 2 was done with High Performance Liquid Chromatography. Picroside 1 ranged from 2.72 to 2.88 mg/capsule and picroside 2 from 5.50 to 6.00 mg/capsule. In the galactosamine-induced liver injury in rats, Pk at a dose of 200 mg/kg p.o. showed a significant reduction (p < 0.05 in liver lipid content, GOT and GPT. In a randomised, double-blind placebo controlled trial in patients diagnosed to have acute viral hepatitis (HBsAg negative, Pk root powder 375 mg three times a day was given for 2 weeks (n = 15 or a matching placebo (n = 18 was given. Difference in values of bilirubin, SGOT and SGPT was significant between placebo and Pk groups. The time in days required for total serum bilirubin to drop to average value of 2.5 mg% was 75.9 days in placebo as against 27.44 days in Pk group. The present study has shown a biological plausability of efficacy of Pk as supported by clinical trial in viral hepatitis, hepatoprotection in animal model and an approach for standardizing extracts based on picroside content.

  15. NGS Transcriptomes and Enzyme Inhibitors Unravel Complexity of Picrosides Biosynthesis in Picrorhiza kurroa Royle ex. Benth.

    Directory of Open Access Journals (Sweden)

    Kirti Shitiz

    Full Text Available Picrorhiza kurroa is an important medicinal herb valued for iridoid glycosides, Picroside-I (P-I and Picroside-II (P-II, which have several pharmacological activities. Genetic interventions for developing a picroside production platform would require knowledge on biosynthetic pathway and key control points, which does not exist as of today. The current study reports that geranyl pyrophosphate (GPP moiety is mainly contributed by the non-mevalonate (MEP route, which is further modified to P-I and P-II through phenylpropanoid and iridoid pathways, in total consisting of 41 and 35 enzymatic steps, respectively. The role of the MEP pathway was ascertained through enzyme inhibitors fosmidomycin and mevinolin along with importance of other integrating pathways using glyphosate, aminooxy acetic acid (AOA and actinomycin D, which overall resulted in 17%-92% inhibition of P-I accumulation. Retrieval of gene sequences for enzymatic steps from NGS transcriptomes and their expression analysis vis-à-vis picrosides content in different tissues/organs showed elevated transcripts for twenty genes, which were further shortlisted to seven key genes, ISPD, DXPS, ISPE, PMK, 2HFD, EPSPS and SK, on the basis of expression analysis between high versus low picrosides content strains of P. kurroa so as to eliminate tissue type/ developmental variations in picrosides contents. The higher expression of the majority of the MEP pathway genes (ISPD, DXPS and ISPE, coupled with higher inhibition of DXPR enzyme by fosmidomycin, suggested that the MEP route contributed to the biosynthesis of P-I in P. kurroa. The outcome of the study is expected to be useful in designing a suitable genetic intervention strategy towards enhanced production of picrosides. Possible key genes contributing to picroside biosynthesis have been identified with potential implications in molecular breeding and metabolic engineering of P. kurroa.

  16. NGS Transcriptomes and Enzyme Inhibitors Unravel Complexity of Picrosides Biosynthesis in Picrorhiza kurroa Royle ex. Benth.

    Science.gov (United States)

    Shitiz, Kirti; Sharma, Neha; Pal, Tarun; Sood, Hemant; Chauhan, Rajinder S

    2015-01-01

    Picrorhiza kurroa is an important medicinal herb valued for iridoid glycosides, Picroside-I (P-I) and Picroside-II (P-II), which have several pharmacological activities. Genetic interventions for developing a picroside production platform would require knowledge on biosynthetic pathway and key control points, which does not exist as of today. The current study reports that geranyl pyrophosphate (GPP) moiety is mainly contributed by the non-mevalonate (MEP) route, which is further modified to P-I and P-II through phenylpropanoid and iridoid pathways, in total consisting of 41 and 35 enzymatic steps, respectively. The role of the MEP pathway was ascertained through enzyme inhibitors fosmidomycin and mevinolin along with importance of other integrating pathways using glyphosate, aminooxy acetic acid (AOA) and actinomycin D, which overall resulted in 17%-92% inhibition of P-I accumulation. Retrieval of gene sequences for enzymatic steps from NGS transcriptomes and their expression analysis vis-à-vis picrosides content in different tissues/organs showed elevated transcripts for twenty genes, which were further shortlisted to seven key genes, ISPD, DXPS, ISPE, PMK, 2HFD, EPSPS and SK, on the basis of expression analysis between high versus low picrosides content strains of P. kurroa so as to eliminate tissue type/ developmental variations in picrosides contents. The higher expression of the majority of the MEP pathway genes (ISPD, DXPS and ISPE), coupled with higher inhibition of DXPR enzyme by fosmidomycin, suggested that the MEP route contributed to the biosynthesis of P-I in P. kurroa. The outcome of the study is expected to be useful in designing a suitable genetic intervention strategy towards enhanced production of picrosides. Possible key genes contributing to picroside biosynthesis have been identified with potential implications in molecular breeding and metabolic engineering of P. kurroa.

  17. Isolation and antitrichomonal activity of the chemical constituents of the leaves of Maytenus phyllanthoides Benth. (Celastraceae)

    Energy Technology Data Exchange (ETDEWEB)

    Moo-Puc, Juan Alberto; Martin-Quintal, Zhelmy; Miron-Lopez, Gumersindo; Mena-Rejon, Gonzalo J., E-mail: mrejon@uady.mx [Laboratorio de Quimica Farmaceutica. Facultad de Quimica. Universidad Autonoma de Yucatan (Mexico); Moo-Puc, Rosa Esther [Unidad de Investigacion Medica Yucatan, Instituto Mexicano del Seguro Social, Yucatan (Mexico); Quijano, Leovigildo [Instituto de Quimica, Universidad Nacional Autonoma de Mexico, Coyoacan (Mexico)

    2014-07-01

    Cyclolignan (+)-lyoniresinol (1), veratric acid (2), vanillic acid (3), lupeol, oleanolic acid, 3β-hydroxy-urs-11-en-28,13β-lactone (4), the mixture of α- and β-amyrin, trans-polyisoprene, and β-sitosterol were isolated from the leaves of Maytenus phyllanthoides. The structures of the isolated compounds were established based on spectroscopic data, mainly {sup 1}H and {sup 13}C nuclear magnetic resonance (NMR). Compound 1, its acetate analog 1a, and compounds 2, 3, and 4 were tested against Trichomonas vaginalis. (+)-Lyoniresinol showed activity corresponding to IC{sub 50} 17.57 μM. This is the first report on the occurrence of 3β-hydroxy-urs-11-en-28,13β-lactone (4) in the Celastraceous family and lyoniresinol in the Maytenus genus, and on the antitrichomonal activity of lyoniresinol. (author)

  18. Arsenic toxicity in Acacia mangium willd. and mimosa Caesalpiniaefolia benth. seedlings

    Directory of Open Access Journals (Sweden)

    Henrique Nery Cipriani

    2013-10-01

    Full Text Available Acacia mangium and Mimosa caesalpiniaefolia are fast-growing woody fabaceous species that might be suitable for phytoremediation of arsenic (As-contaminated sites. To date, few studies on their tolerance to As toxicity have been published. Therefore, this study assessed As toxicity symptoms in A. mangium and M. caesalpiniaefolia seedlings under As stress in a greenhouse. Seedlings of Acacia mangium and M. caesalpiniaefolia were grown for 120 d in an Oxisol-sand mixture with 0, 50, 100, 200, and 400 mg kg-1 As, in four replications in four randomized blocks. The plants were assessed for visible toxicity symptoms, dry matter production, shoot/root ratio, root anatomy and As uptake. Analyses of variance and regression showed that the growth of A. mangium and M. caesalpiniaefolia was severely hindered by As, with a reduction in dry matter production of more than 80 % at the highest As rate. The root/shoot ratio increased with increasing As rates. At a rate of 400 mg kg-1 As, whitish chlorosis appeared on Mimosa caesalpiniaefolia seedlings. The root anatomy of both species was altered, resulting in cell collapse, death of root buds and accumulation of phenolic compounds. Arsenic concentration was several times greater in roots than in shoots, with more than 150 and 350 mg kg-1 in M. caesalpiniaefolia and A. mangium roots, respectively. These species could be suitable for phytostabilization of As-contaminated sites, but growth-stimulating measures should be used.

  19. Una nueva variedad de centrosema Macrocarpum Benth. (Leguminoae: Papilionoideae de los Andes Colombianos

    Directory of Open Access Journals (Sweden)

    Schultze Kraft Rainer

    1995-11-01

    Full Text Available A new variety of Centrosema macrocarpum is described. It occurs only in Colombia, mainly at intermediate altitudes (1000-2000 m in the Andes and the Sierra Nevada de Santa Marta. Diagnostic features of the variety are the reddish colour of young leaves, the purpleviolaceous colour of the corolla, and the unusually large pods and seeds. Una nueva variedad de Centrosema macrocarpum es descrita. Su distribución se concentra sobre todo en el piso térmico medio en la franja altitudinal de 1000-2000 m en los Andes colombianos y la Sierra Nevada de Santa Marta. Son características diagnósticas de la variedad el color rojizo de las hojas jóvenes, el color morado-violáceo de la corola y el tamaño extremadamente grande de las legumbres y semillas.

  20. A pharmacognostic study of Vernonia guineensis Benth. (Asteraceae) : bioactivity, safety, and phytochemical analysis

    NARCIS (Netherlands)

    Toyang, Ngeh Joseph

    2014-01-01

    The world continues to be plagued by numerous diseases and health complications. Unfortunately, current drug discovery efforts are lagging behind compared to the pace of emergence of new diseases or the development of resistance to current therapies. Medicinal plants especially for people in