Sample records for hybridoma formation clonability

  1. Enhancement of hybridoma formation, clonability and cell proliferation in a nanoparticle-doped aqueous environment

    Directory of Open Access Journals (Sweden)

    Karnieli Ohad


    Full Text Available Abstract Background The isolation and production of human monoclonal antibodies is becoming an increasingly important pursuit as biopharmaceutical companies migrate their drug pipelines away from small organic molecules. As such, optimization of monoclonal antibody technologies is important, as this is becoming the new rate-limiting step for discovery and development of new pharmaceuticals. The major limitations of this system are the efficiency of isolating hybridoma clones, the process of stabilizing these clones and optimization of hybridoma cell secretion, especially for large-scale production. Many previous studies have demonstrated how perturbations in the aqueous environment can impact upon cell biology. In particular, radio frequency (RF irradiation of solutions can have dramatic effects on behavior of solutions, cells and in particular membrane proteins, although this effect decays following removal of the RF. Recently, it was shown that nanoparticle doping of RF irradiated water (NPD water produced a stabilized aqueous medium that maintained the characteristic properties of RF irradiated water for extended periods of time. Therefore, the ordering effect in water of the RF irradiation can now be studied in systems that required prolonged periods for analysis, such as eukaryotic cell culture. Since the formation of hybridoma cells involves the formation of a new membrane, a process that is affected by the surrounding aqueous environment, we tested these nanoparticle doped aqueous media formulations on hybridoma cell production. Results In this study, we tested the entire process of isolation and production of human monoclonal antibodies in NPD water as a means for further enhancing human monoclonal antibody isolation and production. Our results indicate an overall enhancement of hybridoma yield, viability, clonability and secretion. Furthermore, we have demonstrated that immortal cells proliferate faster whereas primary human fibroblasts

  2. Increased human hybridoma formation by electrofusion of human B cells with heteromyeloma SPAM-8 cells. (United States)

    Panova, I; Gustafsson, B


    A fusion protocol was designed for the optimal production of hybridomas following electrofusion of human B cells with cells of the heteromyeloma fusion partner SPAM-8. Peripheral blood lymphocytes showed an average fusion efficiency of 0.4 x 10(-4) whereas Epstein-Barr virus-transformed B cells showed fusion efficiencies ranging from 6.2 x 10(-4) to 9.0 x 10(-4). Similar results were obtained with bone marrow-derived lymphocytes. Trypsin treatment of the cells prior to electrofusion further increased the fusion efficiency to 12.3 x 10(-4). In comparison, conventional polyethylene glycol-induced fusion resulted in a fusion efficiency of 0.8 x 10(-4). Thus, electrofusion of human B cells with SPAM-8 heteromyeloma cells introduced a 15-fold increase in hybridoma formation as compared to the conventional fusion method.

  3. Analysis of T cell hybridomas. IV. Characterization of inducible suppressor cell hybridomas



    The Ts3 subset of suppressor cells is generated after antigen priming, but, in order to express suppressor activity these cells require an additional activation step involving triggering with specific suppressor factors (TsF2). This report characterizes two cloned hybridoma cell lines (pTs3 hybridomas) that represent this stage of Ts3 cell differentiation. These hybridoma cells could be specifically activated with TsF2 to release another antigen-specific suppressor factor (TsF3) within 6 h. T...

  4. Serum-free hybridoma culture: ethical, scientific and safety considerations. (United States)

    Even, Megha S; Sandusky, Chad B; Barnard, Neal D


    Despite considerable progress in the development of cell culture techniques, including the development of the serum- and protein-free media that now routinely support hybridoma and mammalian cell growth, fetal bovine serum (FBS) supplemented media are still commonly used: a practice that raises ethical, scientific and safety concerns. The use of FBS in hybridoma culture media is examined here, with regards to the development and production of monoclonal antibodies (mAbs), and it is our recommendation that researchers adopt serum-free cell culture methods to reduce animal use in this area.

  5. Hybridoma fusion cell lines contain an aberrant kappa transcript. (United States)

    Carroll, W L; Mendel, E; Levy, S


    The V region sequence of a non-productive kappa transcript from two myeloma fusion partners has been determined. This transcript has an aberrant VJ recombination site resulting in a translation stop site at position 105. It is variably expressed in hybridomas made from all fusion partners derived from the original MOPC-21 tumor. The amount of this transcript may greatly exceed levels of the productive light chain mRNA.

  6. Label-free hybridoma cell culture quality control by a chip-based impedance flow cytometer. (United States)

    Pierzchalski, Arkadiusz; Hebeisen, Monika; Mittag, Anja; Bocsi, Jozsef; Di Berardino, Marco; Tarnok, Attila


    Impedance flow cytometry (IFC) was evaluated as a possible alternative to fluorescence-based methods for on-line quality monitoring of hybridoma cells. Hybridoma cells were cultured at different cell densities and viability was estimated by means of IFC and fluorescence-based flow cytometry (FCM). Cell death was determined by measuring the impedance phase value at high frequency in low conductivity buffer. IFC data correlate well with reference FCM measurements using AnnexinV and 7-AAD staining. Hybridoma cells growing at different densities in cell culture revealed a density-dependent subpopulation pattern. Living cells of high density cultures show reduced impedance amplitudes, indicating particular cellular changes. Dead cell subpopulations become evident in cultures with increasing cell densities. In addition, a novel intermediate subpopulation, which most probably represents apoptotic cells, was identified. These results emphasize the extraordinary sensitivity of high frequency impedance measurements and their suitability for hybridoma cell culture quality control.

  7. Immunogenomic engineering of a plug-and-(dis)play hybridoma platform. (United States)

    Pogson, Mark; Parola, Cristina; Kelton, William J; Heuberger, Paul; Reddy, Sai T


    Hybridomas, fusions of primary mouse B cells and myelomas, are stable, rapidly-proliferating cell lines widely utilized for antibody screening and production. Antibody specificity of a hybridoma clone is determined by the immunoglobulin sequence of the primary B cell. Here we report a platform for rapid reprogramming of hybridoma antibody specificity by immunogenomic engineering. Here we use CRISPR-Cas9 to generate double-stranded breaks in immunoglobulin loci, enabling deletion of the native variable light chain and replacement of the endogenous variable heavy chain with a fluorescent reporter protein (mRuby). New antibody genes are introduced by Cas9-targeting of mRuby for replacement with a donor construct encoding a light chain and a variable heavy chain, resulting in full-length antibody expression. Since hybridomas surface express and secrete antibodies, reprogrammed cells are isolated using flow cytometry and cell culture supernatant is used for antibody production. Plug-and-(dis)play hybridomas can be reprogrammed with only a single transfection and screening step.


    NARCIS (Netherlands)


    Mice were immunized with purified infectious bronchitis virus (IBV), strain M41. Spleen cells, expanded in vitro by stimulation with M41, were immortalized by fusion to obtain T-cell hybridomas, and two major histocompatability complex (MHC) class II (I-E)-restricted T-cell hybridomas were selected

  9. Identification of Immunoglobulin Gene Sequences from a Small Read Number of mRNA-Seq Using Hybridomas.

    Directory of Open Access Journals (Sweden)

    Yuki Kuniyoshi

    Full Text Available Identification of immunoglobulin genes in hybridomas is essential for producing antibodies for research and clinical applications. A couple of methods such as RACE and degenerative PCR have been developed for determination of the Igh and Igl/Igk coding sequences (CDSs but it has been difficult to process a number of hybridomas both with accuracy and rapidness. Here, we propose a new strategy for antibody sequence determination by mRNA-seq of hybridomas. We demonstrated that hybridomas highly expressed the Igh and Igl/Igk genes and that de novo transcriptome assembly using mRNA-seq data enabled identification of the CDS of both Igh and Igl/Igk accurately. Furthermore, we estimated that only 30,000 sequenced reads are required to identify immunoglobulin sequences from four different hybridoma clones. Thus, our approach would facilitate determining variable CDSs drastically.

  10. Identification of T-cell stimulating antigens from Giardia lamblia by using Giardia-specific T-cell hybridomas. (United States)

    Astiazaran-Garcia, H; Quintero, J; Vega, R; Briceño, P; Oviedo, C; Rascon, L; Garibay-Escobar, A; Castillo-Yañez, F J; Robles-Zepeda, R; Hernandez, J; Velazquez, C


    T-cell immune response plays an important role in controlling Giardia lamblia infections. Little is known about the G. lamblia-specific antigens that stimulate a cell-mediated immune response. The aim of the present study was to identify T-cell stimulating G. lamblia antigens. For this purpose, we generated a group of Giardia-specific T-cell hybridomas (2F9, 4D5, 6D10, 8B9, 9B10, 10F7 and 10G5). Hybridomas were screened for reactivity with G. lamblia protein extract by the CTLL bioassay. These T-cell hybridomas did not exhibit any significant activation either in the absence of G. lamblia protein extract or in the presence of irrelevant antigen (hen white egg lysozyme). To further characterize the T-cell hybridomas generated, we selected three hybridomas (10G5, 4D5 and 9B10). Giardia lamblia proteins of 90-110, 65-77 and 40-64 kDa showed T-cell stimulating activity for the hybridomas 10G5, 4D5 and 9B10, respectively, in a concentration-dependent manner. Protein extract obtained from different G. lamblia strains (GS/M-83-H7, WB C6 and a clinical isolate (YJJ)) stimulated all T-cell hybridomas, indicating that T-cell-stimulating antigens are expressed among different G. lamblia strains. In conclusion, we identified T-cell stimulating G. lamblia antigens by using Giardia-specific T-cell hybridomas. To our knowledge, these hybridomas are the first-described T-cell hybridomas specific for G. lamblia.

  11. Human chromosome 21 determines growth factor dependence in human/mouse B-cell hybridomas

    NARCIS (Netherlands)

    Ebeling, S. B.; Bos, H. M.; Slater, R.; Overkamp, W. J.; Cuthbert, A. P.; Newbold, R. F.; Zdzienicka, M. Z.; Aarden, L. A.


    Interleukin 6 (IL-6) serves as a growth factor for mouse plasmacytomas. As a model for IL-6-mediated growth of plasmacytomas, we study IL-6-dependent B-cell hybridomas, which can be generated through fusion of B lymphocytes with a plasmacytoma cell line, e.g., SP2/0. In the present report, we have

  12. Cell cycle analysis of interleuklin-6 Stimulated B9 hybridoma cell ...

    African Journals Online (AJOL)

    Interleukin-6 is a multifunctional Cytokine. In-vitro, interleukin-6 (IL-6) stimulated murine hybridoma B9 cell proliferation is well established. Cadmium inhibition of this response to IL-6 has been previously reported. Cell cycle analysis of IL-6 stimulated B9 cells in the presence or absence of cadmium (Cd) was performed in ...

  13. Rapid characterization of hybridomas producing monoclonal antibodies against platelet β3 integrin using ELIspot. (United States)

    Maenner, Denis; Werth, Silke; Bein, Gregor; Santoso, Sentot


    Generally, B-cell responses against human platelet antigens are assessed by the serological detection of specific platelet antibodies, mostly against β3 integrin. However, this approach seems to be of low sensitivity, since platelet autoantibodies against αIIbβ3 are detected in only 50% of all patients with immune thrombocytopenia (ITP). In this study, a novel B-cell ELIspot method was established to characterize the specificity of mouse monoclonal antibodies (moabs) against human β3 integrin. Moabs produced by hybridomas were immobilized on membrane and bound antibodies were visualized as spots using biotinylated recombinant proteins αIIbβ3 or αvβ3 and the enzyme labeled streptavidin-substrate system. Three hybridomas, Gi5, Gi16 and AP3, designated previously as anti-αIIbβ3, anti-αIIb and anti-β3, respectively, were investigated. Hybridoma producing moab against CD177 was used as the negative control. Whereas AP3 reacted with αIIbβ3 and αvβ3, Gi5 only formed spots with αIIbβ3. Titration analysis showed that the number of spots correlated significantly with the number of seeded cells. Approximately 15 antibody producing hybridoma cells could be identified among 103 nonproducing B-cells. Furthermore, superior correlation with the total number of IgG producing cells was obtained. Analysis of the third hybridoma, Gi16 (anti-αIIb), showed only few spots with αIIbβ3, indicating that this hybridoma contained different clones (producer and non-producer). Significant increased number of spots could be identified after re-cloning of these clones by limiting dilution method. Our results demonstrate that this B-cell ELIspot assay can be used for the identification of a small number of hybridoma cells producing moabs against β3 integrin, verification of their monoclonality, productivity and for determining their specificity in the early state of workup steps. In the future, this approach may be useful to define B-cell clones in patients who developed

  14. How-To-Do-It: Immunological Assays for the Classroom II--Hybridoma Technology: Production of Monoclonal Antibodies. (United States)

    Russo, A. J.


    Presented is a sample hybridoma assay which can be used in a research or classroom laboratory setting for instructional purposes. Described are experimental methods, materials, and observations made during this activity. (CW)

  15. Screening hybridomas for anabolic androgenic steroids by steroid analog antigen microarray. (United States)

    Du, Hongwu; Chen, Guangyu; Bian, Yongzhong; Xing, Cenzan; Ding, Xue; Zhu, Mengliang; Xun, Yiping; Chen, Peng; Zhou, Yabin; Li, Shaoxu


    Currently, dozens of anabolic androgenic steroids (AAS) are forbidden in the World Anti-Doping Agency Prohibited List, however, despite extensive investigation, there are still lots of AAS without corresponding monoclonal antibodies. A steroid analog antigen microarray made up of ten AAS was fabricated to screen the hybridoma and it was found an original unsuccessful clone turned out to be a candidate anti-boldenone antibody, without any cross-reactions with endogenous AAS or 44 different AAS standard reference materials tested. Our findings suggested that steroid analog antigen microarray could be a promising tool to screen and characterize new applications of antibodies for structure analogs, and this also exhibits the potential to fast identify effective epitopes of hybridomas in a single assay.

  16. Macroscopic Dynamic Modeling of Sequential Batch Cultures of Hybridoma Cells: An Experimental Validation

    Directory of Open Access Journals (Sweden)

    Laurent Dewasme


    Full Text Available Hybridoma cells are commonly grown for the production of monoclonal antibodies (MAb. For monitoring and control purposes of the bioreactors, dynamic models of the cultures are required. However these models are difficult to infer from the usually limited amount of available experimental data and do not focus on target protein production optimization. This paper explores an experimental case study where hybridoma cells are grown in a sequential batch reactor. The simplest macroscopic reaction scheme translating the data is first derived using a maximum likelihood principal component analysis. Subsequently, nonlinear least-squares estimation is used to determine the kinetic laws. The resulting dynamic model reproduces quite satisfactorily the experimental data, as evidenced in direct and cross-validation tests. Furthermore, model predictions can also be used to predict optimal medium renewal time and composition.

  17. Human anti-Dectin-1 antibody, hybridoma producing said antibody and applications thereof


    Kremer, Leonor; Llorente Gómez, María de las Mercedes; Casasnovas, José María; Fernández Ruíz, Elena; Galán Díez, Marta


    [EN] The invention relates to hybridoma MGD3 and the monoclonal antibody produced thereby (also called MGD3), which specifically recognises the human Dectin-1 membrane receptor. Antibody MGD3 is capable of inhibiting the binding of Dectin-1 to the natural ligand thereof, the ss-glucans that are components of the fungal wall. In addition, the aforementioned antibody specifically blocks binding to Candida albicans and the secretion of cytokines induced thereby. The MGD3 antibody obtained enable...

  18. Diversity of the antibody response to tetanus toxoid: comparison of hybridoma library to phage display library.

    Directory of Open Access Journals (Sweden)

    Mahsa Sorouri

    Full Text Available Monoclonal antibodies are important tools in research and since the 1990s have been an important therapeutic class targeting a wide variety of diseases. Earlier methods of mAb production relied exclusively on the lengthy process of making hybridomas. The advent of phage display technology introduced an alternative approach for mAb production. A potential concern with this approach is its complete dependence on an in vitro selection process, which may result in selection of V(H-V(L pairs normally eliminated during the in vivo selection process. The diversity of V(H-V(L pairs selected from phage display libraries relative to an endogenous response is unknown. To address these questions, we constructed a panel of hybridomas and a phage display library using the spleen of a single tetanus toxoid-immunized mouse and compared the diversity of the immune response generated using each technique. Surprisingly, the tetanus toxoid-specific antibodies produced by the hybridoma library exhibited a higher degree of V(H-V(L genetic diversity than their phage display-derived counterparts. Furthermore, the overlap among the V-genes from each library was very limited. Consistent with the notion that accumulation of many small DNA changes lead to increased antigen specificity and affinity, the phage clones displayed substantial micro-heterogeneity. Contrary to previous reports, we found that antigen specificity against tetanus toxoid is encoded by both V(κ and V(H genes. Finally, the phage-derived tetanus-specific clones had a lower binding affinity than the hybridomas, a phenomenon thought to be the result of random pairing of the V-genes.

  19. Barcoded sequencing workflow for high throughput digitization of hybridoma antibody variable domain sequences. (United States)

    Chen, Yongmei; Kim, Si Hyun; Shang, Yonglei; Guillory, Joseph; Stinson, Jeremy; Zhang, Qing; Hötzel, Isidro; Hoi, Kam Hon


    Since the invention of Hybridoma technology by Milstein and Köhler in 1975, its application has greatly advanced the antibody discovery process. The technology enables both functional screening and long-term archival of the immortalized monoclonal antibody producing B cells. Despite the dependable cryopreservation technology for hybridoma cells, practicality of long-term storage has been outpaced by recent progress in robotics and automations, which enables routine identification of thousands of antigen specific hybridoma clones. Such throughput increase imposes two nascent challenges in the antibody discovery process, namely limited cryopreservation storage space and limited throughput in conventional antibody sequencing. We herein provide a barcoded sequencing workflow that utilizes next generation sequencing to expand the conventional sequencing capacity. Accompanied with the bioinformatics tools we describe, the barcoded sequencing workflow robustly reports unambiguous antibody sequences as confirmed with Sanger sequencing controls. In complement with the commonly accessible recombinant DNA technology, the barcoded sequencing workflow allows for high throughput digitization of the antibody sequences and provides an effective solution to the limitations imposed by physical storage and sequencing capacity. Copyright © 2018 Genentech, Inc. Published by Elsevier B.V. All rights reserved.

  20. Processing of Lysozyme by Macrophages: Identification of the Determinant Recognized by Two T-Cell Hybridomas (United States)

    Allen, Paul M.; Strydom, Daniel J.; Unanue, Emil R.


    The purpose of this study was to identify the fragment of the hen egg-white lysozyme (HEL) molecule presented by macrophages to helper T cells. This was investigated by using T-cell hybridomas and macrophages prefixed in paraformaldehyde. We previously had shown that such prefixed macrophages could present a tryptic digest of HEL. The tryptic peptides were separated by HPLC and tested for their ability to stimulate the T-cell hybridomas. Only one tryptic peptide was found to be immunogenic. This immunogenic peptide was identified as the tryptic peptide T-8, containing amino acids 46-61. The precise determinant on the peptide T-8 being recognized was further defined by testing the response of the two T-cell hybridomas to human lysozyme. Neither clone responded to human lysozyme. From the amino acid sequence of human lysozyme, the determinant was localized to the four amino-terminal residues. Cleavage of the immunogenic peptide with either chymotrypsin or protease V-8 completely abolished the immunogenicity. This suggested that the T-cell determinant is located in the hydrophilic amino-terminal residues and that it must be associated with a hydrophobic stretch of amino acids, which allows the peptide to associate with the macrophage plasma membrane.

  1. Mimicking the germinal center reaction in hybridoma cells to isolate temperature-selective anti-PEG antibodies. (United States)

    Su, Yu-Cheng; Al-Qaisi, Talal S; Tung, Hsin-Yi; Cheng, Tian-Lu; Chuang, Kuo-Hsiang; Chen, Bing-Mae; Roffler, Steve R


    Modification of antibody class and binding properties typically requires cloning of antibody genes, antibody library construction, phage or yeast display and recombinant antibody expression. Here, we describe an alternative "cloning-free" approach to generate antibodies with altered antigen-binding and heavy chain isotype by mimicking the germinal center reaction in antibody-secreting hybridoma cells. This was accomplished by lentiviral transduction and controllable expression of activation-induced cytidine deaminase (AID) to generate somatic hypermutation and class switch recombination in antibody genes coupled with high-throughput fluorescence-activated cell sorting (FACS) of hybridoma cells to detect altered antibody binding properties. Starting from a single established hybridoma clone, we isolated mutated antibodies that bind to a low-temperature structure of polyethylene glycol (PEG), a polymer widely used in nanotechnology, biotechnology and pharmaceuticals. FACS of AID-infected hybridoma cells also facilitated rapid identification of class switched variants of monoclonal IgM to monoclonal IgG. Mimicking the germinal center reaction in hybridoma cells may offer a general method to identify and isolate antibodies with altered binding properties and class-switched heavy chains without the need to carry out DNA library construction, antibody engineering and recombinant protein expression.

  2. Development of mouse hybridomas for production of monoclonal antibodies specific to Burkholderia mallei and Burkholderia pseudomallei. (United States)

    Feng, Shaw-Huey; Tsai, Shien; Rodriguez, Jose; Newsome, Tamara; Emanuel, Peter; Lo, Shyh-Ching


    Burkholderia mallei and B. pseudomallei are designated category B biothreat agents on the "select agents" list established by the NIH and CDC. Development of monoclonal antibodies (MAbs) that could effectively differentiate these two closely related species of bacteria and other non-pathogenic Burkholderia bacteria is urgently needed. Splenocytes from mice immunized with various antigen preparations from either B. mallei (American Type Culture Collection [ATCC] 23344) or B. pseudomallei (ATCC 23343) were used for production of hybridomas. Using a three-step cross-screening protocol, a total of 10 hybridomas were selected that produced MAbs which specifically recognized B. mallei 23344 but did not bind B. pseudomallei, Pseudomonas aeruginasa, or any of the other nine Burkholderia species tested. All 10 MAbs targeted to the lipopolysaccharide (LPS) molecules of B. mallei and reacted strongly with 12 out of 15 different strains of B. mallei tested. A total of 14 hybridomas that produced MAbs reacting with B. pseudomallei 23343, but not with B. mallei, P. aeruginasa, or any other nine non-pathogenic Burkholderia species were also selected. All 14 MAbs appeared to react with a proteinase K-sensitive 200-kDa band by immunoblotting analysis. Surprisingly, these 14 MAbs that were raised against the ATCC 23343 strain failed to react to any of the other 13 different strains of B. pseudomallei examined. In conclusion, our B. mallei-specific MAbs can effectively recognize 80% of the different B. mallei strains tested, and all the B. pseudomallei-specific MAbs appeared to react with a unique antigen present only in the ATCC 23343 strain, but not in any other strains of B. pseudomallei tested.

  3. Development of Human Monoclonal Antibodies Against Respiratory Syncytial Virus Using a High Efficiency Human Hybridoma Technique. (United States)

    Alvarado, Gabriela; Crowe, James E


    Human monoclonal antibodies against RSV have high potential for use as prophylaxis or therapeutic molecules, and they also can be used to define the structure of protective epitopes for rational vaccine design. In the past, however, isolation of human monoclonal antibodies was difficult and inefficient. Here, we describe contemporary methods for activation and proliferation of primary human memory B cells followed by cytofusion to non-secreting myeloma cells by dielectrophoresis to generate human hybridomas secreting RSV-specific monoclonal antibodies. We also provide experimental methods for screening human B cell lines to obtain RSV-specific lines, especially lines secreting neutralizing antibodies.

  4. An ELISA for screening hybridoma cultures for monoclonal antibodies against a detergent solubilized integral membrane protein. (United States)

    Noteboom, W D; Knurr, K E; Kim, H S; Richmond, W G; Martin, A P; Vorbeck, M L


    A method is described for the binding of a detergent solubilized integral membrane protein to polystyrene immunoassay plates. Addition of Bouin's fluid, a histochemical fixative, to wells of plates containing the detergent solubilized antigen, followed by low speed centrifugation, is sufficient to promote binding of antigen in the presence of Triton X-100 concentrations as high as 1.75%. The binding of antigen is rapid and the entire binding procedure, including removal of fixative and washing of the plates, can be accomplished in less than 15 min. Immunological specificity of the bound antigen is retained. This method has been used to effectively screen hybridoma cultures for specific antibodies.

  5. Photodynamic response of an endothelial hybridoma cell line using zinc(II) tetrasubstituted phthalocyanines (United States)

    Cruse-Sawyer, Janet E.; Dixon, B.; Roberts, David J.; Griffiths, John; Brown, Stanley B.


    The EAhy 926 cell is a hybridoma line derived from human endothelium and A549/8 cells. They display stable endothelial characteristics and may provide an indication of how endothelial cells respond to photodynamic therapy. Cells were grown as monolayers, seeded at a density of 104 cells/35 mm dish, and then incubated with zinc (II) tetrasubstituted phthalocyanines (carboxylated, sulphonated, pyridinium or diethanolamine sulphonamide). After 24 hours, the cells were illuminated with laser light at 680 nm or 692 nm as appropriate. The response to each photosensitizer was evaluated using cell proliferation, clonogenicity, and release of tissue factor.

  6. Preformed purified peptide/major histocompatibility class I complexes are potent stimulators of class I-restricted T cell hybridomas

    DEFF Research Database (Denmark)

    Stryhn, A; Pedersen, L O; Ortiz-Navarrete, V


    A panel of antigen-specific, major histocompatibility complex class I-restricted T cell hybridomas has been generated to examine the capacity of peptide/class I complexes to stimulate T cells at the molecular level. Peptide/class I complexes were generated in detergent solution, purified...... and quantitated. Latex particles were subsequently coated with known amounts of preformed complexes and used to stimulate the T cell hybridomas. Stimulation was specific, i.e. only the appropriate peptide/class I combination were stimulatory, and quite sensitive, i.e. as little as 300 complexes per bead could...... expression, suggesting that antigen-specific stimulation of class I-restricted T cell hybridomas, as assessed by IL-2 release, does not depend on CD8....

  7. The influence of cellular seeding density in the microencapsulation of hybridoma cells. (United States)

    Arús, L; Orive, G; Hernández, R; Rodriguez, A; Rojas, A; Pedraz, J L


    The aim of this study was to assess the influence of different seeding densities on the function of hybridoma cells (clone 1B5, IgG 2alpha) producing an anti-angiogenic monoclonal antibody (mAb), microencapsulated using a high-voltage electrostatic field. Viable cells were microencapsulated in alginate/poly-L-lysine/alginate (APA) capsules and maintained in tissue culture. Cellular growth rates, production and release of mAb from the capsules were assessed. This study shows that hybridoma cells survive, proliferate and remain functionally competent for over one month in vitro after microencapsulation in APA capsules generated in an electrostatic field. However, the cell seeding density had to be at least 10(7) cells/ml for the microencapsulated cells to be viable and to produce and release mAb through the capsule membrane. The maximum monoclonal antibody concentration in this culture was 29.1 microg/ml by day 17, with a tendency to increase, but capsule breakage impeded the follow-up of this determination.

  8. Lysis-free separation of hybridoma cells by continuous disc stack centrifugation. (United States)

    Tebbe, H; Lütkemeyer, D; Gudermann, F; Heidemann, R; Lehmann, J


    The non-destructive removal of hybridoma cells from fermentation broth with an improved disc stack centrifuge (CSA1, Westfalia Separator AG, Oelde, Germany) was investigated. The centrifuge was equipped with a hydrohermetic feed system, which allowed a gentle, shearless acceleration of the cells inside the bowl. No significant cell damage was observed during the separation of hybridoma cells from repeated batch fermentation in 100 liter scale. In the clarified liquid phase there was no increase in Lactate-Dehydrogenase (LDH) activity. Consequently, there was no increased exposure of the product to intracellular components.Due to continuous operation with a periodic and automatic discharge of sediment, a high throughput was achieved without any considerable loss of product. The clarification for mammalian cells was in the range of 99% to 99.9%, depending on the operating conditions. The content of cell debris and other small particles decreased about 30 to 50%, depending on the particle load in the feed stream. The centrifuge was fully contained; cleaning and sterilizing in place possible. Therefore, the decice could be integrated easily into the fermentation process.

  9. A point-addressable transfer system for automated sampling, feeding, and expansion of hybridoma cultures. (United States)

    Karu, A E; Miller, P L; Chase, C; Cornutt, W


    A Dynatech Autoprep liquid sampling system has been modified to perform fully automated aseptic sampling, feeding, and expansion of hybridoma cultures in standard 96- and 24-well culture plates. The system is controlled by an Apple IIe computer, and uses a single teflon probe to transfer culture medium from randomly located wells to EIA plates and deliver fresh medium to the sampled wells. An 'expansion mode' allows suspension of cells for transfer to another plate. The sampling probe may be washed with sterile medium, buffer, or water between each transfer. Any combination of up to 6 assay plates, sterile growth plates, and expansion plates may be operated on at one time, and each transaction is recorded on a floppy disk file. Experiments with various hybridoma cultures indicated that transfers were reproducible, sterility was maintained, and the washing procedure reduced cross-contamination of cultures with other cells or antibodies to negligible levels. The APPLE BASIC computer programs which perform the functions and record the transactions are described in the paper and the Appendix, and are available upon request.

  10. Effects of BCL-2 over-expression on B cells in transgenic rats and rat hybridomas. (United States)

    Iscache, Anne-Laure; Ménoret, Séverine; Tesson, Laurent; Rémy, Séverine; Usal, Claire; Pedros, Christophe; Saoudi, Abdelhadi; Buelow, Roland; Anegon, Ignacio


    The rat is an important biomedical experimental model that benefited from the recent development of new transgenic and knockout techniques. With the goal to optimize rat mAb production and to analyze the impact of Bcl-2 on B-cell development, we generated bcl-2 transgenic rats. Transgenic rats showed Bcl-2 over-expression in B cells, increased B cell numbers in lymphoid organs, elevated production of immunoglobulins (Igs) and prolonged B-cell survival in vitro. Transgenic rats remained healthy, reproduced normally and did not develop autoimmunity. Fusions with bcl-2 transgenic splenocytes did not result in increased hybridoma generation. A comparison of on- and off-rates of 39 mAbs generated with bcl-2 transgenic and wild-type animals revealed no significant differences. Over-expression of Bcl-2 in hybridomas did not change cell proliferation but resulted in increased Ig production. Bcl-2 transgenic rats will be a useful tool for the generation of rat mAbs, the analysis of B cells in different pathophysiological models, such as autoimmunity, cancer or organ transplantation, and the study of rat B-cell biology.

  11. Increased production of antigen-specific lymphocytes-b during invitro immunization using carrier-specific t-helper hybridomas

    NARCIS (Netherlands)

    Schilizzi, B. M.; Kroesen, B.-J.; The, T. Hauw; de Leij, L.


    An in vitro method to increase the production of hapten-specific antibody-forming B cells (AFC) using a carrier-specific T helper hybridoma and murine splenocytes is described. Naive splenocytes (6 X 10(6)/ml) are cultured in vitro in the presence of a hapten-carrier conjugate (DNP.OVA) and

  12. A fast and simple dot-immunobinding assay for quantifiction of mouse immunoglobulins in hybridoma culture supernatants

    Czech Academy of Sciences Publication Activity Database

    Sulimenko, Tetyana; Dráber, Pavel


    Roč. 289, - (2004), s. 89-95 ISSN 0022-1759 R&D Projects: GA AV ČR IBS5052301; GA MŠk LN00A026; GA MŠk 1P04OE158 Keywords : dot-immunobinding assay * hybridoma culture superntatants * mouse immunoglobulins Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 2.464, year: 2004

  13. Kinetic analysis of hybridoma cells viability under mechanical shear stress with and without serum protection. (United States)

    Legazpi, Lorea; Laca, Adriana; Díaz, Mario


    The effect of a well-defined mild shear stress on hybridoma cell viability (HB-8852) in a serum-free culture medium has been analysed, and the role as shear protector of different concentrations of fetal bovine serum have been studied. Samples harvested from cultures in their late exponential growth phase, were subjected in a rheometer to a constant shear stress of 0.41 +/- 0.02 Pa, and the evolution of viable and total cell concentrations was determined and compared with static controls. A simple segregated kinetic model for the viable and dead cells was used to know the effect of serum concentration on the specific cell growth and death rate of the cells.

  14. Isolation of hybridomas secreting monoclonal antibodies against Physalia physalis (Portuguese man-o'war) nematocyst venom. (United States)

    Gaur, P K; Anthony, R L; Calton, G J; Burnett, J W


    Balb/C mice were immunized with crude Portuguese Man-O'War (Physalia physalis) nematocyst venom and their spleen immunocytes were fused with plasmacytoma cells. Nine hybridomas which produced IgG specific for Man-O'War venom were identified using a specific ELISA technique. Ammonium sulfate and DEAE cellulose-purified monoclonal anti-venom antibody had an ELISA titer of 1:4000 and an ability to neutralize the lethal activity (4 LD50/0.6 ml ascites fluid) of an i.v. challenge of crude venom. Indirect immunofluorescence testing demonstrated that the monoclonal antibody isolated in these experiments reacted against a venom component located in the nematocyst wall and thread.

  15. Three-dimensional culture for monoclonal antibody production by hybridoma cells immobilized in macroporous gel particles. (United States)

    Nilsang, Suthasinee; Nehru, Vishal; Plieva, Fatima M; Nandakumar, Kutty Selva; Rakshit, Sudip Kumar; Holmdahl, Rikard; Mattiasson, Bo; Kumar, Ashok


    Cell proliferation and long-term production of monoclonal antibody IgG(2b) by M2139 hybridoma cells immobilized in macroporous gel particles (MGPs) in packed-bed reactor were studied for a period of 60 days. The MGPs were made of supermacroporous gels produced in frozen conditions from crosslinked polyacrylamide and modified with gelatin which were housed in special plastic carriers (7 x 9 mm(2)). Cells were trapped in the interior part of MGPs by attaching to the void space of the gel matrix as three-dimensional (3D) cultivation using gelatin as a substrate layer. Optimizing productivity by hybridoma cell relies on understanding regulation of antibody production. In this study, the behavior of M2139 cells in two-dimensional cultures on multiwell plate surfaces was also investigated. The effect of three different medium such as basal medium Dulbecco's modified Eagle's medium (D-MEM) containing L-glutamine or L-glutamine + 2 mM alpha-ketoglutarate or L-alanyl-glutamine (GlutaMAXtrade mark) was studied prior to its use in 3D cultivation. The kinetics of cell growth in basal medium containing L-glutamine + alpha-ketoglutarate was similar to cells grown on GlutaMAX containing medium, whereas D-MEM containing L-glutamine showed lower productivity. With the maximal viable cell density (6.85 x 10(6) cells mL(-1)) and highest specific mAb production rate (3.9 mug mL(-1) 10(-4) viable cell day(-1)), D-MEM-GlutaMAX was further selected for 3D cultivation. Cells in MGPs were able to grow and secrete antibody for 30 days in packed-bed batch reactor, before a fresh medium reservoir was replaced. After being supplied with fresh medium, cells again showed continuous growth for another 30 days with mAb production efficiency of 50%. These results demonstrate that MGPs can be used efficiently as supporting carrier for long-term monoclonal antibody production.

  16. Reliable cloning of functional antibody variable domains from hybridomas and spleen cell repertoires employing a reengineered phage display system. (United States)

    Krebber, A; Bornhauser, S; Burmester, J; Honegger, A; Willuda, J; Bosshard, H R; Plückthun, A


    A prerequisite for the use of recombinant antibody technologies starting from hybridomas or immune repertoires is the reliable cloning of functional immunoglobulin genes. For this purpose, a standard phage display system was optimized for robustness, vector stability, tight control of scFv-delta geneIII expression, primer usage for PCR amplification of variable region genes, scFv assembly strategy and subsequent directional cloning using a single rare cutting restriction enzyme. This integrated cloning, screening and selection system allowed us to rapidly obtain antigen binding scFvs derived from spleen-cell repertoires of mice immunized with ampicillin as well as from all hybridoma cell lines tested to date. As representative examples, cloning of monoclonal antibodies against a his tag, leucine zippers, the tumor marker EGP-2 and the insecticide DDT is presented. Several hybridomas whose genes could not be cloned in previous experimental setups, but were successfully obtained with the present system, expressed high amounts of aberrant heavy and light chain mRNAs, which were amplified by PCR and greatly exceeded the amount of binding antibody sequences. These contaminating variable region genes were successfully eliminated by employing the optimized phage display system, thus avoiding time consuming sequencing of non-binding scFv genes. To maximize soluble expression of functional scFvs subsequent to cloning, a compatible vector series to simplify modification, detection, multimerization and rapid purification of recombinant antibody fragments was constructed.

  17. Identification and verification of hybridoma-derived monoclonal antibody variable region sequences using recombinant DNA technology and mass spectrometry. (United States)

    Babrak, Lmar; McGarvey, Jeffery A; Stanker, Larry H; Hnasko, Robert


    Antibody engineering requires the identification of antigen binding domains or variable regions (VR) unique to each antibody. It is the VR that define the unique antigen binding properties and proper sequence identification is essential for functional evaluation and performance of recombinant antibodies (rAb). This determination can be achieved by sequence analysis of immunoglobulin (Ig) transcripts obtained from a monoclonal antibody (MAb) producing hybridoma and subsequent expression of a rAb. However the polyploidy nature of a hybridoma cell often results in the added expression of aberrant immunoglobulin-like transcripts or even production of anomalous antibodies which can confound production of rAb. An incorrect VR sequence will result in a non-functional rAb and de novo assembly of Ig primary structure without a sequence map is challenging. To address these problems, we have developed a methodology which combines: 1) selective PCR amplification of VR from both the heavy and light chain IgG from hybridoma, 2) molecular cloning and DNA sequence analysis and 3) tandem mass spectrometry (MS/MS) on enzyme digests obtained from the purified IgG. Peptide analysis proceeds by evaluating coverage of the predicted primary protein sequence provided by the initial DNA maps for the VR. This methodology serves to both identify and verify the primary structure of the MAb VR for production as rAb. Published by Elsevier Ltd.

  18. A cell sorter with modified bamboo charcoal for the efficient selection of specific antibody-producing hybridomas. (United States)

    Lin, Chien-Chen; Ni, Mei-Hui; Chang, Yu-Chung; Yeh, Hsiu-Lun; Lin, Feng-Huei


    Monoclonal antibodies (mAbs) have been proven useful in research and clinical applications. However, the generation of mAbs by conventional hybridoma technology is time-, cost- and labor-consuming. Here we developed a simplified procedure for efficient generation and selection of antibody-producing hybridomas within 1 h, using a particular cell sorter design, a cytoflow reactor-based cell sorter (CBCS) which consists mainly of the "cytoflow reactor" that comprises two components, a reaction chamber and a glass tubing for air and medium exchange by gravity, and the "sorting material", human EGFR-conjugated bamboo charcoal, for specific B-cell enrichment. The high surface area and porous structure of bamboo charcoal greatly increased cell density and protein production. Moreover, from Raman, FT-IR spectroscopy and IFA analysis, the carboxylation and immobilization of bamboo charcoal can be introduced easily by nitric acid treatment and conjugated handily with human EGFR using EDC/NHS. Other evidences, such as IFA, showed that the specific hybridomas generated in this study could secrete specific anti-human EGFR antibodies. Our design allows the production of mAbs while avoiding time-consuming steps, such as large numbers of limiting dilutions and screening assays, and demonstrates that the CBCS could be a powerful tool for monoclonal antibody production. Copyright (c) 2010 Elsevier Ltd. All rights reserved.

  19. A Rapid Method to Characterize Mouse IgG Antibodies and Isolate Native Antigen Binding IgG B Cell Hybridomas.

    Directory of Open Access Journals (Sweden)

    Haolin Liu

    Full Text Available B cell hybridomas are an important source of monoclonal antibodies. In this paper, we developed a high-throughput method to characterize mouse IgG antibodies using surface plasmon resonance technology. This assay rapidly determines their sub-isotypes, whether they bind native antigen and their approximate affinities for the antigen using only 50 μl of hybridoma cell culture supernatant. Moreover, we found that mouse hybridomas secreting IgG antibodies also have membrane form IgG expression without Igα. Based on this surface IgG, we used flow cytometry to isolate rare γ2a isotype switched variants from a γ2b antibody secreting hybridoma cell line. Also, we used fluorescent antigen to single cell sort antigen binding hybridoma cells from bulk mixture of fused hybridoma cells instead of the traditional multi-microwell plate screening and limiting dilution sub-cloning thus saving time and labor. The IgG monoclonal antibodies specific for the native antigen identified with these methods are suitable for in vivo therapeutic uses, but also for sandwich ELISA assays, histology, flow cytometry, immune precipitation and x-ray crystallography.

  20. On chip real time monitoring of B-cells hybridoma secretion of immunoglobulin. (United States)

    Milgram, Sarah; Cortes, Sandra; Villiers, Marie-Bernadette; Marche, Patrice; Buhot, Arnaud; Livache, Thierry; Roupioz, Yoann


    The secretions of molecules by cells are of tremendous interest for both fundamental insights studies and medical purposes. In this study, we propose a new biochip-based approach for the instantaneous monitoring of protein secretions, using antibody production by B lymphocytes cultured in vitro. This was possible thanks to the Surface Plasmon Resonance imaging (SPRi) of a protein biochip where antigen proteins (Hen Egg Lysozyme, HEL) were micro-arrayed along with series of control proteins. B cell hybridomas were cultured on the chip and the secretion of immunoglobulins (antibody) specific to HEL was monitored in real-time and detected within only few minutes rather than after a 30-60 min incubation with standard ELISA experiments. This fast and sensitive detection was possible thanks to the sedimentation of the cells on the biochip sensitive surface, where local antibody concentrations are much higher before dilution in the bulk medium. An other interesting feature of this approach for the secretion monitoring was the independence of the SPR response--after normalization--regarding to the density of the surface-immobilized probes. Such biosensor might thus pave the way to new tools capable of both qualitative and semi-quantitative analysis of proteins secreted by other immune cells. Copyright © 2010 Elsevier B.V. All rights reserved.

  1. Establishment and characterization of mouse bone marrow-derived mast cell hybridomas

    Energy Technology Data Exchange (ETDEWEB)

    Kawahara, Takeshi, E-mail: [Integrated Department of Sciences of Functional Foods, Graduate School of Agriculture, Shinshu University, Nagano (Japan)


    Interleukin (IL)-3-dependent mouse bone marrow-derived mast cells (BMMCs) are an important model for studying the function of mucosal-type mast cells. In the present study, BMMCs were successfully immortalized by cell fusion using a hypoxanthine-aminopterin-thymidine medium-sensitive variant of P815 mouse mastocytoma (P815-6TgR) as a partner cell line. The established mouse mast cell hybridomas (MMCHs) expressed {alpha}, {beta}, and {gamma} subunits of high-affinity immunoglobulin E (IgE) receptor (Fc{epsilon}RI) and possessed cytoplasmic granules devoid of or partially filled with electron-dense material. Four independent MMCH clones continuously proliferated without supplemental exogenous IL-3 and showed a degranulation response on stimulation with IgE+antigen. Furthermore, histamine synthesis and release by degranulation were confirmed in MMCH-D5, a MMCH clone that showed the strongest degranulation response. MMCH-D5 exhibited elevated levels of IL-3, IL-4, IL-13, granulocyte-macrophage colony-stimulating factor, tumor necrosis factor (TNF)-{alpha}, and cyclooxygenase 2, and production of prostaglandin D{sub 2} and leukotriene C{sub 4} in response to IgE-induced stimulation. MMCH clones also expressed Toll-like receptors (TLRs) 1, 2, 4, and 6 and showed elevated levels of TNF-{alpha} expression in response to stimulation with TLR2 and TLR4 ligands. The MMCHs established using this method should be suitable for studies on Fc{epsilon}RI- and TLR-mediated effector functions of mast cells.

  2. Comparison between Hybridoma and Fab/phage Anti-RhD: Their V Gene Usage and Pairings

    Directory of Open Access Journals (Sweden)

    W. S. Perera


    Full Text Available Our 11 anti-RhD's in conjunction with 37 previously published RhD antibodies, produced by hybridoma technology were analysed for germline gene usage and restriction in VH and VL pairings. The 17 VH germline genes used by the hybridoma anti-RhD IgG were derived from 4 VH families (VH1, VH2, VH3 and VH4. Eighteen kappa chains were restricted to only 5 germline genes from only 2 Vκ families (Vκ1 and κ3. However, the 13 lambda chains were not as restricted, using 10 Vλ germline genes from 4 families (Vλ1, Vλ2, Vλ3 and Vλ8. Fifty six unique Fab/phage anti-RhD were also analysed. In all cases the Fab/phage VH germline genes were derived from the VH3 family (41/41. The 29 kappa chains were restricted to 4 germline genes primarily from Vκ1 (97% germline genes from 5 families (Vλ1, Vλ2, Vλ3, Vλ4 and Vλ7. The VH germline genes of the Fab/phage were restricted to 4 of the 17 used by the hybridoma anti-RhD IgG (DP46, DP49, DP50 and DP77. Ninety percent of the Fab/phage were restricted to 1 of the 5 Vκ germline genes used by the IgG (DPK9. However, the repertoire of the Vλ germline genes used in these two systems is different, with analysis showing greater diversity in Vλ gene usage with 8 unique germline genes used by 76% Fab/phage compared to 4 unique genes used by 46% hybriboma anti-RhD.

  3. Radically altered T cell receptor signaling in glycopeptide-specific T cell hybridoma induced by antigen with minimal differences in the glycan group

    DEFF Research Database (Denmark)

    Jensen, T; Nielsen, M; Gad, Monika


    A T cell hybridoma raised against the synthetic glycopeptide T(72)(Tn) was used to study whether the initial TCR signaling events are markedly different when the hybridoma is stimulated with glycopeptides closely related to the cognate glycopeptide antigen. T(72)(Tn) has an alpha-D-GalNAc group O......-linked to the central threonine in the decapeptide VITAFTEGLK, and the hybridoma is known to be highly specific for this carbohydrate group. T(72)(Tn)-pulsed APC induced tyrosine phosphorylation of the TCR-zeta 21- and 23-kDa proteins and the downstream p42/44 MAP kinase and strong IL-2 secretion. APC pulsed with T(72...

  4. Development of novel mouse hybridomas producing monoclonal antibodies specific to human and mouse nucleolar protein SURF-6. (United States)

    Polzikov, Mikhail A; Kordyukova, Maria Yu; Zavalishina, Larisa E; Magoulas, Charalambos; Zatsepina, Olga V


    SURF-6 is an evolutionarily conserved nucleolar protein that is important for cell viability; however, its function in mammals still remains uncertain. The aim of this study is to generate monoclonal antibodies to human SURF-6 protein suitable for fundamental and biomedical research. The full-size human SURF-6 was expressed as a recombinant GST-fusion protein and used as an antigen to generate monoclonal antibodies, S79 and S148, specific for SURF-6. The monoclonal antibody produced by hybridoma clone S79 specifically recognizes endogenous SURF-6 by Western and immunofluorescence analyses in various cultured human cells, and by immunohistochemistry in paraffin-embedded sections of human breast cancer samples. Moreover, S79 immunoprecipitates protein complexes containing SURF-6 from HeLa cells extracts. The antibody S79 recognizes SURF-6 only in human cells; however, the antibody produced by hybridoma clone S148 can detect SURF-6 of human and mouse origin. Monoclonal antibodies to the nucleolar protein SURF-6 described in this work can be a useful tool for studies of ribosome biogenesis in normal and cancer cells.

  5. A method for the transformation of hybridoma cell lines with improved efficiency : its use in the production of bispecific monoclonal antibodies

    NARCIS (Netherlands)

    Bos, R.; Berg, E. van den; Nieuwenhuizen, W.


    A method was investigated to transduce a specific drug resistance marker, coded on a bacterial plasmid construct, to a hybridoma cell line using electroporation. We tested the influence of several buffers and the form of the DNA on cell viability and stable transfection frequency. We found a tenfold

  6. High affinity humanized antibodies without making hybridomas; immunization paired with mammalian cell display and in vitro somatic hypermutation.

    Directory of Open Access Journals (Sweden)

    Audrey D McConnell

    Full Text Available A method has been developed for the rapid generation of high-affinity humanized antibodies from immunized animals without the need to make conventional hybridomas. Rearranged IgH D(J regions were amplified from the spleen and lymph tissue of mice immunized with the human complement protein C5, fused with a limited repertoire of human germline heavy chain V-genes to form intact humanized heavy chains, and paired with a human light chain library. Completed heavy and light chains were assembled for mammalian cell surface display and transfected into HEK 293 cells co-expressing activation-induced cytidine deaminase (AID. Numerous clones were isolated by fluorescence-activated cell sorting, and affinity maturation, initiated by AID, resulted in the rapid evolution of high affinity, functional antibodies. This approach enables the efficient sampling of an immune repertoire and the direct selection and maturation of high-affinity, humanized IgGs.

  7. Formats

    Directory of Open Access Journals (Sweden)

    Gehmann, Ulrich


    Full Text Available In the following, a new conceptual framework for investigating nowadays’ “technical” phenomena shall be introduced, that of formats. The thesis is that processes of formatting account for our recent conditions of life, and will do so in the very next future. It are processes whose foundations have been laid in modernity and which will further unfold for the time being. These processes are embedded in the format of the value chain, a circumstance making them resilient to change. In addition, they are resilient in themselves since forming interconnected systems of reciprocal causal circuits.Which leads to an overall situation that our entire “Lebenswelt” became formatted to an extent we don’t fully realize, even influencing our very percep-tion of it.

  8. Complete dissection of the Hb(64-76) determinant using T helper 1, T helper 2 clones, and T cell hybridomas

    DEFF Research Database (Denmark)

    Evavold, B D; Williams, S G; Hsu, B L


    We have generated cloned Th1 cells, Th2 cells, and T cell hybridomas specific for the single immunogenic peptide from the beta-chain of murine hemoglobin (Hb(64-76)). The availability of these various types of T cells provided us an unique opportunity to examine and dissect the T cell response...... to an immunogenic peptide. A panel of altered Hb peptides was made by replacing each amino acid in the Hb peptide (positions 64-76) with a conservative amino acid substitution or an alanine. Although none of the eleven T cell clones and hybridomas tested exhibited the same pattern of reactivity to the substituted...... Hb peptides, some general features were identified for all T cell responses. The primary T cell contact residue of Hb(64-76) was shown to be asparagine 72. For every Hb(64-76) specific T cell, no activation was observed using a peptide containing the conservative substitution of a glutamine...

  9. Viability and proliferation of L929, tumour and hybridoma cells in the culture media containing sericin protein as a supplement or serum substitute. (United States)

    Cao, Ting-Ting; Zhang, Yu-Qing


    Cell cultures often require the addition of animal serum and other supplements. In this study, silk sericin, a bioactive protein, recovered from the waste of silk floss production was hydrolysed into three pepsin-degraded sericin peptides with different ranges of molecular mass. Normal animal cells, tumour cells and hybridoma cells were cultured systematically in FBS culture media containing sericin as a supplement or serum substitute. The culture test and microscopic observation of L929 cells showed that the smaller molecular weight of the degraded sericin is most suitable for cell culture. The cell culture results showed that with the degradation of sericin, for normal mouse fibroblast L929 cells, addition of 0.75 % sericin into FBS culture medium yields cell viability that is superior to FBS culture medium alone. When all serum was replaced by sericin, cell viability in the sericin medium could reach about one half of that in FBS medium. When in a medium containing a mixture of FBS: sericin (6:4, v/v), the cell culture effect is about 80 %. For the cultures of four tumour and one hybridoma cells, regardless of the molecular weight range, these degraded sericin peptides could substitute all serum in FBS media. The cell viability and proliferation of these tumour and hybridoma cells are equivalent or superior to that in FBS medium. In other words, cell viability and proliferation of these tumour and hybridoma cells in sericin media are more preferable to serum media. The mechanism of the sericin protein to promote cell growth and proliferation will be further investigated later.

  10. Cloning and Characterization of a Hybridoma Secreting a 4-(Methylnitrosamino-1-(3-pyridyl-1-butanone (NNK-Specific Monoclonal Antibody and Recombinant F(ab

    Directory of Open Access Journals (Sweden)

    Lawrence K. Silbart


    Full Text Available Smokeless tobacco products have been associated with increased risks of oro-pharyngeal cancers, due in part to the presence of tobacco-specific nitrosamines (TSNAs such as 4-(methylnitrosamino-1-(3-pyridyl-1-butanone (NNK. These potent carcinogens are formed during tobacco curing and as a result of direct nitrosation reactions that occur in the oral cavity. In the current work we describe the isolation and characterization of a hybridoma secreting a high-affinity, NNK-specific monoclonal antibody. A structurally-related benzoyl derivative was synthesized to facilitate coupling to NNK-carrier proteins, which were characterized for the presence of the N-nitroso group using the Griess reaction, and used to immunize BALB/c mice. Splenocytes from mice bearing NNK-specific antibodies were used to create hybridomas. Out of four, one was selected for subcloning and characterization. Approximately 99% of the monoclonal antibodies from this clone were competitively displaced from plate-bound NNKB conjugates in the presence of free NNK. The affinity of the monoclonal antibody to the NNKB conjugates was Kd = 2.93 nM as determined by surface plasmon resonance. Free nicotine was a poor competitor for the NNKB binding site. The heavy and light chain antibody F(ab fragments were cloned, sequenced and inserted in tandem into an expression vector, with an FMDV Furin 2A cleavage site between them. Expression in HEK 293 cells revealed a functional F(ab with similar binding features to that of the parent hybridoma. This study lays the groundwork for synthesizing transgenic tobacco that expresses carcinogen-sequestration properties, thereby rendering it less harmful to consumers.

  11. Identification of Eps15 as antigen recognized by the monoclonal antibodies aa2 and ab52 of the Wuerzburg Hybridoma Library against Drosophila brain.

    Directory of Open Access Journals (Sweden)

    Partho Halder

    Full Text Available The Wuerzburg Hybridoma Library against the Drosophila brain represents a collection of around 200 monoclonal antibodies that bind to specific structures in the Drosophila brain. Here we describe the immunohistochemical staining patterns, the Western blot signals of one- and two-dimensional electrophoretic separation, and the mass spectrometric characterization of the target protein candidates recognized by the monoclonal antibodies aa2 and ab52 from the library. Analysis of a mutant of a candidate gene identified the Drosophila homolog of the Epidermal growth factor receptor Pathway Substrate clone 15 (Eps15 as the antigen for these two antibodies.

  12. Identification and structural characterization of interneurons of the Drosophila brain by monoclonal antibodies of the würzburg hybridoma library.

    Directory of Open Access Journals (Sweden)

    Beatriz Blanco Redondo

    Full Text Available Several novel synaptic proteins have been identified by monoclonal antibodies (mAbs of the Würzburg hybridoma library generated against homogenized Drosophila brains, e.g. cysteine string protein, synapse-associated protein of 47 kDa, and Bruchpilot. However, at present no routine technique exists to identify the antigens of mAbs of our library that label only a small number of cells in the brain. Yet these antibodies can be used to reproducibly label and thereby identify these cells by immunohistochemical staining. Here we describe the staining patterns in the Drosophila brain for ten mAbs of the Würzburg hybridoma library. Besides revealing the neuroanatomical structure and distribution of ten different sets of cells we compare the staining patterns with those of antibodies against known antigens and GFP expression patterns driven by selected Gal4 lines employing regulatory sequences of neuronal genes. We present examples where our antibodies apparently stain the same cells in different Gal4 lines suggesting that the corresponding regulatory sequences can be exploited by the split-Gal4 technique for transgene expression exclusively in these cells. The detection of Gal4 expression in cells labeled by mAbs may also help in the identification of the antigens recognized by the antibodies which then in addition to their value for neuroanatomy will represent important tools for the characterization of the antigens. Implications and future strategies for the identification of the antigens are discussed.

  13. Software sensors for the monitoring of perfusion cultures: evaluation of the hybridoma density and the medium composition from glucose concentration measurements. (United States)

    Pelletier, F; Fonteix, C; da Silva, A L; Marc, A; Engasser, J M


    New software sensors based on the Extended Kalman Filter technique have been developed for the monitoring of animal cell perfusion cultures. They use a kinetic model describing the growth, death and metabolism of hybridoma cells as a function of the medium composition. The model was initially validated on a batch culture and found to correctly predict the continuous perfusion culture kinetics, except for the production of ammonia and lactate. Using the measurement of a single component in the culture medium, in this case glucose, the Extended Kalman Filter provides an excellent evaluation of the time variation of the concentrations of living and dead cells, of glutamine and antibodies, during the whole perfusion culture for a retained cell density rising from 1 to 11 x 10(6) inside the reactor.

  14. Presentation of antigen to T lymphocytes by non-immune B-cell hybridoma clones: evidence for specific and non-specific presentation (United States)

    Cohly, H. H.; Morrison, D. R.; Zouhair Atassi, M. Z.


    Non-immune SJL (H-2s) spleen cells were fused with non-secreting, non-antigen presenting (H-2d) Balb/c 653-myeloma cells and the hybridomas were cloned by two limiting dilutions. The resulting hybrid B-cell clones were tested for their antigen presentation capability to SJL T-cell lines that were specific for either lysozyme or myoglobin. In proliferative assays, 53% of the antigen presenting B-cell clones presented both myoglobin and lysozyme (general presenters) while the other 47% presented specifically either myoglobin or lysozyme (specific presenters). The ability to selectively present either myoglobin or lysozyme indicates that antigen presentation at the clonal level can be specific or non-specific depending on the particular B-cell clone.

  15. Sponge Hybridomas: Applications and Implications

    NARCIS (Netherlands)

    Pomponi, S.A.; Jevitt, A.; Patel, J.; Diaz, M.C.


    Many sponge-derived natural products with applications to human health have been discovered over the past three decades. In vitro production has been proposed as one biological alternative to ensure adequate supply of marine natural products for preclinical and clinical development of drugs.

  16. Effect of amino acid supplementation on titer and glycosylation distribution in hybridoma cell cultures-Systems biology-based interpretation using genome-scale metabolic flux balance model and multivariate data analysis. (United States)

    Reimonn, Thomas M; Park, Seo-Young; Agarabi, Cyrus D; Brorson, Kurt A; Yoon, Seongkyu


    Genome-scale flux balance analysis (FBA) is a powerful systems biology tool to characterize intracellular reaction fluxes during cell cultures. FBA estimates intracellular reaction rates by optimizing an objective function, subject to the constraints of a metabolic model and media uptake/excretion rates. A dynamic extension to FBA, dynamic flux balance analysis (DFBA), can calculate intracellular reaction fluxes as they change during cell cultures. In a previous study by Read et al. (2013), a series of informed amino acid supplementation experiments were performed on twelve parallel murine hybridoma cell cultures, and this data was leveraged for further analysis (Read et al., Biotechnol Prog. 2013;29:745-753). In order to understand the effects of media changes on the model murine hybridoma cell line, a systems biology approach is applied in the current study. Dynamic flux balance analysis was performed using a genome-scale mouse metabolic model, and multivariate data analysis was used for interpretation. The calculated reaction fluxes were examined using partial least squares and partial least squares discriminant analysis. The results indicate media supplementation increases product yield because it raises nutrient levels extending the growth phase, and the increased cell density allows for greater culture performance. At the same time, the directed supplementation does not change the overall metabolism of the cells. This supports the conclusion that product quality, as measured by glycoform assays, remains unchanged because the metabolism remains in a similar state. Additionally, the DFBA shows that metabolic state varies more at the beginning of the culture but less by the middle of the growth phase, possibly due to stress on the cells during inoculation. © 2016 American Institute of Chemical Engineers Biotechnol. Prog., 32:1163-1173, 2016. © 2016 American Institute of Chemical Engineers.

  17. Antigen presentation by non-immune B-cell hybridoma clones: presentation of synthetic antigenic sites reveals clones that exhibit no specificity and clones that present only one epitope (United States)

    Cohly, H. H.; Morrison, D. R.; Atassi, M. Z.


    Recently, we reported the preparation and antigen-presenting properties of hybridoma B-cell clones obtained after fusing non-secreting, non-antigen presenting Balb/c 653-myeloma cells with non-immune SJL spleen cells. It was found that antigen presentation at the clonal level can be specific or non-specific, depending on the particular B-cell clone. In the present work, one specific and one general presenter B-cell clones were tested for their epitope presentation ability to SJL T-cells that were specific to lysozyme or myoglobin. B-cell clone A1G12, a general presenter which presented both lysozyme and myoglobin to their respective T-cell lines, was found to present all five myoglobin epitopes while clone A1L16, a lysozyme specific presenter presented only one of the three epitopes of lysozyme. The latter reveals a hitherto unknown submolecular specificity (to a given epitope within a protein) for antigen presenting cells at the clonal level. Therefore, the specificity of T-cell recognition does not only derive from the T-cell but may also be dependent on the epitope specificity of the antigen-presenting B-cell.

  18. Stellar formation

    CERN Document Server

    Reddish, V C


    Stellar Formation brings together knowledge about the formation of stars. In seeking to determine the conditions necessary for star formation, this book examines questions such as how, where, and why stars form, and at what rate and with what properties. This text also considers whether the formation of a star is an accident or an integral part of the physical properties of matter. This book consists of 13 chapters divided into two sections and begins with an overview of theories that explain star formation as well as the state of knowledge of star formation in comparison to stellar structure

  19. Soil formation.

    NARCIS (Netherlands)

    Breemen, van N.; Buurman, P.


    Soil Formation deals with qualitative and quantitative aspects of soil formation (or pedogenesis) and the underlying chemical, biological, and physical processes. The starting point of the text is the process - and not soil classification. Effects of weathering and new formation of minerals,

  20. Galaxy Formation

    DEFF Research Database (Denmark)

    Sparre, Martin

    galaxies form stars throughout the history of the Universe, and secondly it is shown that observations of gamma-ray bursts (GRBs) can be used to probe galaxies with active star formation in the early Universe. A conclusion from the hydrodynamical simulations is that the galaxies from the stateof......Galaxy formation is an enormously complex discipline due to the many physical processes that play a role in shaping galaxies. The objective of this thesis is to study galaxy formation with two different approaches: First, numerical simulations are used to study the structure of dark matter and how...... is important, since it helps constraining chemical evolution models at high redshift. A new project studying how the population of galaxies hosting GRBs relate to other galaxy population is outlined in the conclusion of this thesis. The core of this project will be to quantify how the stellar mass function...

  1. Digtets formater

    DEFF Research Database (Denmark)

    Schmidt, Michael Kallesøe; Rasmussen, Krista Stinne Greve; Skriver, Svend


    Løppenthin, Olga Ravn, Mikkel Thykier, Caspar Eric, and Simon Grotrian are discussed. By using the format as a point of departure rather than applying a more conventional practice of close reading, the authors argue for a broad-spectred approach to literary analysis which focuses on aspects of the conception......The article examines how key terminology of textual criticism can be taken as a starting point for the investigation of material aspects of contemporary poetry. The concepts in question are ‘text’ and ‘work’ as defined by Johnny Kondrup (2013). The authors take the view that the categorical...

  2. Cement Formation

    DEFF Research Database (Denmark)

    Telschow, Samira; Jappe Frandsen, Flemming; Theisen, Kirsten


    Cement production has been subject to several technological changes, each of which requires detailed knowledge about the high multiplicity of processes, especially the high temperature process involved in the rotary kiln. This article gives an introduction to the topic of cement, including...... an overview of cement production, selected cement properties, and clinker phase relations. An extended summary of laboratory-scale investigations on clinkerization reactions, the most important reactions in cement production, is provided. Clinker formations by solid state reactions, solid−liquid and liquid...

  3. Galaxy Formation

    CERN Document Server

    Longair, Malcolm S


    This second edition of Galaxy Formation is an up-to-date text on astrophysical cosmology, expounding the structure of the classical cosmological models from a contemporary viewpoint. This forms the background to a detailed study of the origin of structure and galaxies in the Universe. The derivations of many of the most important results are derived by simple physical arguments which illuminate the results of more advanced treatments. A very wide range of observational data is brought to bear upon these problems, including the most recent results from WMAP, the Hubble Space Telescope, galaxy surveys like the Sloan Digital Sky Survey and the 2dF Galaxy Redshift Survey, studies of Type 1a supernovae, and many other observations.

  4. Habit formation. (United States)

    Smith, Kyle S; Graybiel, Ann M


    Habits, both good ones and bad ones, are pervasive in animal behavior. Important frameworks have been developed to understand habits through psychological and neurobiological studies. This work has given us a rich understanding of brain networks that promote habits, and has also helped us to understand what constitutes a habitual behavior as opposed to a behavior that is more flexible and prospective. Mounting evidence from studies using neural recording methods suggests that habit formation is not a simple process. We review this evidence and take the position that habits could be sculpted from multiple dissociable changes in neural activity. These changes occur across multiple brain regions and even within single brain regions. This strategy of classifying components of a habit based on different brain signals provides a potentially useful new way to conceive of disorders that involve overly fixed behaviors as arising from different potential dysfunctions within the brain's habit network.

  5. Application of a new anti-zearalenone monoclonal antibody in different immunoassay formats. (United States)

    Burmistrova, Natalia A; Goryacheva, Irina Yu; Basova, Evgenia Yu; Franki, Ann-Sophie; Elewaut, Dirk; Van Beneden, Katrien; Deforce, Dieter; Van Peteghem, Carlos; De Saeger, Sarah


    Monoclonal antibodies against zearalenone (ZEA) were raised in mice according to the hybridoma technology and applied in different immunochemical techniques. More specifically, three formats based on the competitive direct enzyme immunoassay principle were developed: an enzyme-linked immunosorbent assay (ELISA), a flow-through gel-based immunoassay column and a flow-through membrane-based immunoassay. In ELISA, the 50% inhibitory concentration (IC50) was 0.8 ng/mL, and the limit of detection for ZEA standard solutions was 0.1 ng/mL. The antibodies showed a high ZEA (100%) and alpha-zearalenol (alpha-ZOL) (69%) recognition, while cross-reactivities with alpha-zearalanol, zearalanone, beta-zearalenol and beta- zearalanol were 42%, 22%, dilution up to 5% and 15% (v/v) of wheat matrix, respectively). The cut-off level of the gel- and membrane-based immunoassays was established at 100 microg/kg. Potentials and limitations of the developed methods were compared. The possible application for multi-mycotoxin analysis of the ELISA method based on a single monoclonal antibody was investigated. Therefore, principal component analysis and partial least squares regression data modelling were used to separate the immunoassay responses of two cross-reactants (ZEA and alpha-ZOL).

  6. Formate Formation and Formate Conversion in Biological Fuels Production

    Directory of Open Access Journals (Sweden)

    Bryan R. Crable


    Full Text Available Biomethanation is a mature technology for fuel production. Fourth generation biofuels research will focus on sequestering CO2 and providing carbon-neutral or carbon-negative strategies to cope with dwindling fossil fuel supplies and environmental impact. Formate is an important intermediate in the methanogenic breakdown of complex organic material and serves as an important precursor for biological fuels production in the form of methane, hydrogen, and potentially methanol. Formate is produced by either CoA-dependent cleavage of pyruvate or enzymatic reduction of CO2 in an NADH- or ferredoxin-dependent manner. Formate is consumed through oxidation to CO2 and H2 or can be further reduced via the Wood-Ljungdahl pathway for carbon fixation or industrially for the production of methanol. Here, we review the enzymes involved in the interconversion of formate and discuss potential applications for biofuels production.

  7. Evidence for Complex Formation of the Bacillus cereus Haemolysin BL Components in Solution

    Directory of Open Access Journals (Sweden)

    Franziska Tausch


    Full Text Available Haemolysin BL is an important virulence factor regarding the diarrheal type of food poisoning caused by Bacillus cereus. However, the pathogenic importance of this three-component enterotoxin is difficult to access, as nearly all natural B. cereus culture supernatants additionally contain the highly cytotoxic Nhe, the second three-component toxin involved in the aetiology of B. cereus-induced food-borne diseases. To better address the toxic properties of the Hbl complex, a system for overexpression and purification of functional, cytotoxic, recombinant (rHbl components L2, L1 and B from E. coli was established and an nheABC deletion mutant was constructed from B. cereus reference strain F837/76. Furthermore, 35 hybridoma cell lines producing monoclonal antibodies (mAbs against Hbl L2, L1 and B were generated. While mAbs 1H9 and 1D8 neutralized Hbl toxicity and thus, represent important tools for future investigations of the mode-of-action of Hbl on the target cell surface, mAb 1D7, in contrast, even enhanced Hbl toxicity by supporting the binding of Hbl B to the cell surface. By using the specific mAbs in Dot blots, indirect and hybrid sandwich enzyme immuno assays (EIAs, complex formation between Hbl L1 and B, as well as L1 and L2 in solution could be shown for the first time. Surface plasmon resonance experiments with the rHbl components confirmed these results with KD values of 4.7 × 10−7 M and 1.5 × 10−7 M, respectively. These findings together with the newly created tools lay the foundation for the detailed elucidation of the molecular mode-of-action of the highly complex three-component Hbl toxin.

  8. Evidence for Complex Formation of the Bacillus cereus Haemolysin BL Components in Solution (United States)

    Tausch, Franziska; Dietrich, Richard; Janowski, Robert; Märtlbauer, Erwin; Jessberger, Nadja


    Haemolysin BL is an important virulence factor regarding the diarrheal type of food poisoning caused by Bacillus cereus. However, the pathogenic importance of this three-component enterotoxin is difficult to access, as nearly all natural B. cereus culture supernatants additionally contain the highly cytotoxic Nhe, the second three-component toxin involved in the aetiology of B. cereus-induced food-borne diseases. To better address the toxic properties of the Hbl complex, a system for overexpression and purification of functional, cytotoxic, recombinant (r)Hbl components L2, L1 and B from E. coli was established and an nheABC deletion mutant was constructed from B. cereus reference strain F837/76. Furthermore, 35 hybridoma cell lines producing monoclonal antibodies (mAbs) against Hbl L2, L1 and B were generated. While mAbs 1H9 and 1D8 neutralized Hbl toxicity and thus, represent important tools for future investigations of the mode-of-action of Hbl on the target cell surface, mAb 1D7, in contrast, even enhanced Hbl toxicity by supporting the binding of Hbl B to the cell surface. By using the specific mAbs in Dot blots, indirect and hybrid sandwich enzyme immuno assays (EIAs), complex formation between Hbl L1 and B, as well as L1 and L2 in solution could be shown for the first time. Surface plasmon resonance experiments with the rHbl components confirmed these results with KD values of 4.7 × 10−7 M and 1.5 × 10−7 M, respectively. These findings together with the newly created tools lay the foundation for the detailed elucidation of the molecular mode-of-action of the highly complex three-component Hbl toxin. PMID:28926954

  9. The Format Dilemma. (United States)

    Oder, Norman


    Reports results of a survey of public libraries that investigated trends in audiovisual materials. Highlights include format issues; audiobooks; media budgets for various formats; video collections; DVDs; circulation; collection sizes; music CDs; and future possibilities. (LRW)

  10. Formate Formation and Formate Conversion in Biological Fuels Production

    NARCIS (Netherlands)

    Crable, B.R.; Plugge, C.M.; McInerney, M.J.; Stams, A.J.M.


    Biomethanation is a mature technology for fuel production. Fourth generation biofuels research will focus on sequestering CO2 and providing carbon-neutral or carbon-negative strategies to cope with dwindling fossil fuel supplies and environmental impact. Formate is an important intermediate in the

  11. Immunochemical Approach for Monitoring of Structural Transition of ApoA-I upon HDL Formation Using Novel Monoclonal Antibodies. (United States)

    Kimura, Hitoshi; Mikawa, Shiho; Mizuguchi, Chiharu; Horie, Yuki; Morita, Izumi; Oyama, Hiroyuki; Ohgita, Takashi; Nishitsuji, Kazuchika; Takeuchi, Atsuko; Lund-Katz, Sissel; Akaji, Kenichi; Kobayashi, Norihiro; Saito, Hiroyuki


    Apolipoprotein A-I (apoA-I) undergoes a large conformational reorganization during remodeling of high-density lipoprotein (HDL) particles. To detect structural transition of apoA-I upon HDL formation, we developed novel monoclonal antibodies (mAbs). Splenocytes from BALB/c mice immunized with a recombinant human apoA-I, with or without conjugation with keyhole limpet hemocyanin, were fused with P3/NS1/1-Ag4-1 myeloma cells. After the HAT-selection and cloning, we established nine hybridoma clones secreting anti-apoA-I mAbs in which four mAbs recognize epitopes on the N-terminal half of apoA-I while the other five mAbs recognize the central region. ELISA and bio-layer interferometry measurements demonstrated that mAbs whose epitopes are within residues 1-43 or 44-65 obviously discriminate discoidal and spherical reconstituted HDL particles despite their great reactivities to lipid-free apoA-I and plasma HDL, suggesting the possibility of these mAbs to detect structural transition of apoA-I on HDL. Importantly, a helix-disrupting mutation of W50R into residues 44-65 restored the immunoreactivity of mAbs whose epitope being within residues 44-65 against reconstituted HDL particles, indicating that these mAbs specifically recognize the epitope region in a random coil state. These results encourage us to develop mAbs targeting epitopes in the N-terminal residues of apoA-I as useful probes for monitoring formation and remodeling of HDL particles.

  12. The Conic Benchmark Format

    DEFF Research Database (Denmark)

    Friberg, Henrik A.

    This document constitutes the technical reference manual of the Conic Benchmark Format with le extension: .cbf or .CBF. It unies linear, second-order cone (also known as conic quadratic) and semidenite optimization with mixed-integer variables. The format has been designed with benchmark libraries...... in mind, and therefore focuses on compact and easily parsable representations. The problem structure is separated from the problem data, and the format moreover facilitate benchmarking of hotstart capability through sequences of changes....

  13. Formative Assessment Probes (United States)

    Eberle, Francis; Keeley, Page


    Formative assessment probes can be effective tools to help teachers build a bridge between students' initial ideas and scientific ones. In this article, the authors describe how using two formative assessment probes can help teachers determine the extent to which students make similar connections between developing a concept of matter and a…

  14. Exploring Opponent Formats

    DEFF Research Database (Denmark)

    Jensen, Mads Møller; Rasmussen, Majken; Grønbæk, Kaj


    of how the opponent format and relationships impact a game are almost absent in current research. Thus, this paper aims to elucidate how the perception of a competition differs, depending on the opponent format, by presenting a game mechanic framework. The paper furthermore presents an interactive...

  15. Usage Record Format Recommendation

    CERN Document Server

    Nilsen, J.K.; Muller-Pfeerkorn, R


    For resources to be shared, sites must be able to exchange basic accounting and usage data in a common format. This document describes a common format which enables the exchange of basic accounting and usage data from different resources. This record format is intended to facilitate the sharing of usage information, particularly in the area of the accounting of jobs, computing, memory, storage and cloud usage but with a structure that allows an easy extension to other resources. This document describes the Usage Record components both in natural language form and annotated XML. This document does not address how these records should be used, nor does it attempt to dictate the format in which the accounting records are stored. Instead, it denes a common exchange format. Furthermore, nothing is said regarding the communication mechanisms employed to exchange the records, i.e. transport layer, framing, authentication, integrity, etc.

  16. Manuel UNIMARC format bibliographique

    CERN Document Server


    This manual is the French translation of the second edition of UNIMARC Manual: bibliographic format published in English in 1994 and completed by 5 updates published from 1996 to 2005. This 5th French edition is composite. It reproduces identically a part of the 4th edition published in 2002 and, for the fields of the format modified in the Update 5, it offers a new more structured presentation. This is a handbook dedicated to French-speaking users of the UNIMARC format for bibliographic descriptions.

  17. PCF File Format.

    Energy Technology Data Exchange (ETDEWEB)

    Thoreson, Gregory G [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)


    PCF files are binary files designed to contain gamma spectra and neutron count rates from radiation sensors. It is the native format for the GAmma Detector Response and Analysis Software (GADRAS) package [1]. It can contain multiple spectra and information about each spectrum such as energy calibration. This document outlines the format of the file that would allow one to write a computer program to parse and write such files.

  18. Plant Formate Dehydrogenase

    Energy Technology Data Exchange (ETDEWEB)

    John Markwell


    The research in this study identified formate dehydrogenase, an enzyme that plays a metabolic role on the periphery of one-carbon metabolism, has an unusual localization in Arabidopsis thaliana and that the enzyme has an unusual kinetic plasticity. These properties make it possible that this enzyme could be engineered to attempt to engineer plants with an improved photosynthetic efficiency. We have produced transgenic Arabidopsis and tobacco plants with increased expression of the formate dehydrogenase enzyme to initiate further studies.

  19. Characterizing bursty star formation (United States)

    Emami, Najmeh


    An ongoing area of research in galaxy evolution is the efficiency of star formation as a function of galaxy halo mass. At low mass, it is believed that supernova feedback can expel gas from the galaxy and shut down star formation. However, there are still significant uncertainties in how the momentum/energy of the supernova couple with the gas and the efficiency with which it drives winds. Particularly uncertain are the parameters of the resulting bursts of star formation —amplitudes, durations, and periods — with important implications for interpreting observations of dwarf galaxies. Some hydrodynamical simulations predict order of magnitude bursts (and quenching) on very short (indicators of dwarf galaxies (H-alpha and ultraviolet luminosities) that trace star formation on different time scales (~5 Myr and ~20 Myr, respectively), as well as their relation to the average galaxies of similar stellar mass, to better constrain the parameters of the star formation bursts. We find that the burst amplitude increases with decreasing stellar mass, with amplitudes ranging two orders of magnitude at stellar masses of 10^7. We also find that the star formation is quenched very rapidly, with e-folding times less than 10 Myr in galaxies with stellar masses less than 10^(7.5). We conclude by comparing our results to recent hydrodynamical simulations and discussing the effects of stochastic sampling of the stellar initial mass function.

  20. Formation of ball lightning

    Energy Technology Data Exchange (ETDEWEB)

    Silberg, P.A. (2833 Lawton Drive, Amarillo, Texas (USA))

    A plasma continuum model for the formation of ball lightning is developed based on a substantial number of reports that the ball is often in the discharge column of a previous lightning stroke. The usual method of setting up the plasma equation for a one-component electron plasma is used. An approximate equation for the plasma is derived from the describing equation which is then solved exactly in terms of the Jacobi elliptic functions. The formation of the ball is based on a nonlinearity of the plasma equation which under certain circumstances permits the field to collapse into a small region. This collapse is interpreted to be ball lightning. The approximate equation derived for the plasma has the same form as a previous equation used to describe the formation of the fireball plasma.

  1. Galaxy formation and evolution

    CERN Document Server

    Mo, Houjun; White, Simon


    The rapidly expanding field of galaxy formation lies at the interface between astronomy, particle physics, and cosmology. Covering diverse topics from these disciplines, all of which are needed to understand how galaxies form and evolve, this book is ideal for researchers entering the field. Individual chapters explore the evolution of the Universe as a whole and its particle and radiation content; linear and nonlinear growth of cosmic structure; processes affecting the gaseous and dark matter components of galaxies and their stellar populations; the formation of spiral and elliptical galaxies; central supermassive black holes and the activity associated with them; galaxy interactions; and the intergalactic medium. Emphasizing both observational and theoretical aspects, this book provides a coherent introduction for astronomers, cosmologists, and astroparticle physicists to the broad range of science underlying the formation and evolution of galaxies.

  2. Forces in strategy formation

    DEFF Research Database (Denmark)

    Steensen, Elmer Fly; Sanchez, Ron


    literature. Based on an extensive review of relevant theory and empirical work in strategic decision-making, organizational change theory, cognitive and social psychology, and strategy processes, seven kinds of ''forces'' - rational, imposed, teleological, learning, political, heuristic, and social......This chapter proposes that organizational strategy formation should be characterized theoretically as a process that is subject to several interacting forces, rather than represented by separate discrete decisionmodels or theoretic perspectives, as is commonly done in the strategic management...... - are identified as interacting in and having significant influence on the strategy formation process. It is further argued that by applying a holistic ''forces-view'' of the significant and interacting influences on strategy formation, we can better understand the dynamics and challenges in managing the process...

  3. Principles of star formation

    CERN Document Server

    Bodenheimer, Peter H


    Understanding star formation is one of the key fields in present-day astrophysics. This book treats a wide variety of the physical processes involved, as well as the main observational discoveries, with key points being discussed in detail. The current star formation in our galaxy is emphasized, because the most detailed observations are available for this case. The book presents a comparison of the various scenarios for star formation, discusses the basic physics underlying each one, and follows in detail the history of a star from its initial state in the interstellar gas to its becoming a condensed object in equilibrium. Both theoretical and observational evidence to support the validity of the general evolutionary path are presented, and methods for comparing the two are emphasized. The author is a recognized expert in calculations of the evolution of protostars, the structure and evolution of disks, and stellar evolution in general. This book will be of value to graduate students in astronomy and astroph...

  4. Control of Ice Formation. (United States)

    Lo, Ching-Wen; Sahoo, Venkataraman; Lu, Ming-Chang


    Ice formation is a catastrophic problem affecting our daily life in a number of ways. At present, deicing methods are costly, inefficient, and environmentally unfriendly. Recently, the use of superhydrophobic surfaces has been suggested as a potential passive anti-icing method. However, no surface is able to repel frost formation at a very cold temperature. In this work, we demonstrated the abilities of spatial control of ice formation and confinement of the ice-stacking direction. The control and confinement were achieved by manipulating the local free energy barrier for frosting. The V-shaped microgroove patterned surface, which possessed these abilities, exhibited the best anti-icing and deicing performances among the studied surfaces. The insight of this study can be applied to alleviate the impact of icing on our daily life and in many industrial systems.

  5. Situated Formative Feedback

    DEFF Research Database (Denmark)

    Lukassen, Niels Bech; Wahl, Christian; Sorensen, Elsebeth Korsgaard

    This study addresses the conceptual challenge of providing students with good quality feedback to enhance student learning in an online community of practice (COP). The aim of the study is to identify feedback mechanisms in a virtual learning environment (VLE) and to create a full formative...... feedback episode (FFE) through an online dialogue. The paper argues that dialogue is crucial for student learning and that feedback is not only something the teacher gives to the student. Viewing good quality feedback as social, situated, formative, emphasis is put on the establishment of dialogue. We...... refer to this type of feedback as, Situated Formative Feedback (SFF). As a basis for exploring, identifying and discussing relevant aspects of SFF the paper analyses qualitative data from a Moodle dialogue. Data are embedded in the qualitative analytic program Nvivo and are analysed with a system...

  6. Tea aroma formation

    Directory of Open Access Journals (Sweden)

    Chi-Tang Ho


    Full Text Available Besides water, tea is one of the most popular beverages around the world. The chemical ingredients and biological activities of tea have been summarized recently. The current review summarizes tea aroma compounds and their formation in green, black, and oolong tea. The flavor of tea can be divided into two categories: taste (non-volatile compounds and aroma (volatile compounds. All of these aroma molecules are generated from carotenoids, lipids, glycosides, etc. precursors, and also from Maillard reaction. In the current review, we focus on the formation mechanism of main aromas during the tea manufacturing process.

  7. Isolating Triggered Star Formation

    Energy Technology Data Exchange (ETDEWEB)

    Barton, Elizabeth J.; Arnold, Jacob A.; /UC, Irvine; Zentner, Andrew R.; /KICP, Chicago /Chicago U., EFI; Bullock, James S.; /UC, Irvine; Wechsler, Risa H.; /KIPAC, Menlo


    Galaxy pairs provide a potentially powerful means of studying triggered star formation from galaxy interactions. We use a large cosmological N-body simulation coupled with a well-tested semi-analytic substructure model to demonstrate that the majority of galaxies in close pairs reside within cluster or group-size halos and therefore represent a biased population, poorly suited for direct comparison to 'field' galaxies. Thus, the frequent observation that some types of galaxies in pairs have redder colors than 'field' galaxies is primarily a selection effect. We use our simulations to devise a means to select galaxy pairs that are isolated in their dark matter halos with respect to other massive subhalos (N= 2 halos) and to select a control sample of isolated galaxies (N= 1 halos) for comparison. We then apply these selection criteria to a volume-limited subset of the 2dF Galaxy Redshift Survey with M{sub B,j} {le} -19 and obtain the first clean measure of the typical fraction of galaxies affected by triggered star formation and the average elevation in the star formation rate. We find that 24% (30.5 %) of these L* and sub-L* galaxies in isolated 50 (30) h{sup -1} kpc pairs exhibit star formation that is boosted by a factor of {approx}> 5 above their average past value, while only 10% of isolated galaxies in the control sample show this level of enhancement. Thus, 14% (20 %) of the galaxies in these close pairs show clear triggered star formation. Our orbit models suggest that 12% (16%) of 50 (30) h{sup -1} kpc close pairs that are isolated according to our definition have had a close ({le} 30 h{sup -1} kpc) pass within the last Gyr. Thus, the data are broadly consistent with a scenario in which most or all close passes of isolated pairs result in triggered star formation. The isolation criteria we develop provide a means to constrain star formation and feedback prescriptions in hydrodynamic simulations and a very general method of understanding

  8. The formation of stars

    CERN Document Server

    Stahler, Steven W


    This book is a comprehensive treatment of star formation, one of the most active fields of modern astronomy. The reader is guided through the subject in a logically compelling manner. Starting from a general description of stars and interstellar clouds, the authors delineate the earliest phases of stellar evolution. They discuss formation activity not only in the Milky Way, but also in other galaxies, both now and in the remote past. Theory and observation are thoroughly integrated, with the aid of numerous figures and images. In summary, this volume is an invaluable resource, both as a text f

  9. Syntactic Formats for Free

    DEFF Research Database (Denmark)

    Klin, Bartek; Sobocinski, Pawel


    A framework of Plotkin and Turi’s, originally aimed at providing an abstract notion of bi-simulation, is modified to cover other operational equivalences and preorders. Combined with bi-algebraic methods, it yields a technique for the derivation of syntactic formats for transition system specific......A framework of Plotkin and Turi’s, originally aimed at providing an abstract notion of bi-simulation, is modified to cover other operational equivalences and preorders. Combined with bi-algebraic methods, it yields a technique for the derivation of syntactic formats for transition system...

  10. Densities and Kinematic Viscosities for the Systems Benzene + Methyl Formate, Benzene + Ethyl Formate, Benzene + Propyl Formate, and Benzene + Butyl Formate

    DEFF Research Database (Denmark)

    Emmerling, Uwe; Rasmussen, Peter


    Densities and kinematic viscosities have been measured for the system benzene + methyl formate at 20°C and for the systems benzene + ethyl formate, benzene + propyl formate, and benzene + butyl formate from 20°C to 50°C. The results for the system benzene + methyl formate have been correlated using...... a Redlich-Kister type of expression with temperature-independent parameters and the data for the systems benzene + ethyl formate, benzene + propyl formate, and benzene + butyl formate with temperature-dependent parameters. The viscosities have furthermore been compared to values predicted by means of the GC...

  11. Carrascolendas: A Formative Evaluation. (United States)

    Laosa, Luis M.

    A formative research project sought to test viewer reactions to two pilot programs of the Carrascolendas series. A total of 360 Puerto Rican-American, Cuban-American, Mexican-American, and Anglo-American children in grades 1, 2, and 3 were observed as they watched the programs. Results indicated that there was high eye contact during the…

  12. Generic file format (United States)

    Felgate, Nick


    The Generic File Format (GFF) is a file format developed within the UK ASW community for the interchange and storage of underwater sonar data. Originally developed for the interchange of time-series data between analysis systems, it has been extended to provide for storage of processed acoustic data (e.g., power and DEMON spectrum, lofargram grey-scale), nonacoustic data (e.g., own-ship dynamics, sensor configuration) and event data (e.g., tracker output, sonar intercepts). The format employs the chunk concept, as used in the WAV and AIFF file formats, to provide extendability (including local variants) while providing a measure of backward compatability. However, the basic concept has been adapted to allow for the mixing in the one file of multiple channels of different sample-rates and data-types through the inclusion of a data frame concept and multiple data blocks. Chunk cross-referencing has been employed to ensure data consistency. A provision is made in the header of the file to store details of the sensor and processing for the data (e.g., the number of hydrophones, beam direction, FFT size) so that an analysis system does not need to know about the sensor or other system from which the data originated.

  13. The Formation of Trihalomethanes. (United States)

    Trussell, R. Rhodes; Umphres, Mark D.


    Reviewed are a number of factors important in the formation of trihalomethanes (THM) including the nature of aquatic humus and the influences of preozonation, bromide, pH, and chlorine. A brief investigation is also conducted into the kinetics of the THM reaction. Several major research needs are represented. (CS)

  14. Meningococcal biofilm formation

    DEFF Research Database (Denmark)

    Lappann, M.; Haagensen, Janus Anders Juul; Claus, H.


    We show that in a standardized in vitro flow system unencapsulated variants of genetically diverse lineages of Neisseria meningitidis formed biofilms, that could be maintained for more than 96 h. Biofilm cells were resistant to penicillin, but not to rifampin or ciprofloxacin. For some strains, m......X alleles was identified among genetically diverse meningococcal strains. PilX alleles differed in their propensity to support autoaggregation of cells in suspension, but not in their ability to support microcolony formation within biofilms in the continuous flow system......., microcolony formation within biofilms was observed. Microcolony formation in strain MC58 depended on a functional copy of the pilE gene encoding the pilus subunit pilin, and was associated with twitching of cells. Nevertheless, unpiliated pilE mutants formed biofilms showing that attachment and accumulation...... of cells did not depend on pilus expression. Mutation and complementation analysis revealed that the type IV pilus-associated protein PilX, which was recently shown to mediate interbacterial aggregation, indirectly supported microcolony formation by contributing to pilus expression. A large number of Pil...

  15. Pattern formation today. (United States)

    Chuong, Cheng-Ming; Richardson, Michael K


    Patterns are orders embedded in randomness. They may appear as spatial arrangements or temporal series, and the elements may appear identical or with variations. Patterns exist in the physical world as well as in living systems. In the biological world, patterns can range from simple to complex, forming the basic building blocks of life. The process which generates this ordering in the biological world was termed pattern formation. Since Wolpert promoted this concept four decades ago, scientists from molecular biology, developmental biology, stem cell biology, tissue engineering, theoretical modeling and other disciplines have made remarkable progress towards understanding its mechanisms. It is time to review and re-integrate our understanding. Here, we explore the origin of pattern formation, how the genetic code is translated into biological form, and how complex phenotypes are selected over evolutionary time. We present four topics: Principles, Evolution, Development, and Stem Cells and Regeneration. We have interviewed several leaders in the field to gain insight into how their research and the field of pattern formation have shaped each other. We have learned that both molecular process and physico-chemical principles are important for biological pattern formation. New understanding will emerge through integration of the analytical approach of molecular-genetic manipulation and the systemic approach of model simulation. We regret that we could not include every major investigator in the field, but hope that this Special Issue of the Int. J. Dev. Biol. represents a sample of our knowledge of pattern formation today, which will help to stimulate more research on this fundamental process.

  16. Formation of multiple networks

    DEFF Research Database (Denmark)

    Magnani, Matteo; Rossi, Luca


    While most research in Social Network Analysis has focused on single networks, the availability of complex on-line data about individuals and their mutual heterogenous connections has recently determined a renewed interest in multi-layer network analysis. To the best of our knowledge, in this paper...... we introduce the first network formation model for multiple networks. Network formation models are among the most popular tools in traditional network studies, because of both their practical and theoretical impact. However, existing models are not sufficient to describe the generation of multiple...... networks. Our model, motivated by an empirical analysis of real multi-layered network data, is a conservative extension of single-network models and emphasizes the additional level of complexity that we experience when we move from a single- to a more complete and realistic multi-network context....

  17. Determinants for gallstone formation

    DEFF Research Database (Denmark)

    Shabanzadeh, Daniel Monsted; Sorensen, Lars Tue; Jørgensen, Torben


    associations were found for blood pressure, smoking, alcohol consumption, HDL cholesterol, or triglycerides in meta-analyses. Conclusions: Age, female sex, BMI, non-HDL cholesterol, and polyps are independent determinants for gallstone formation. Incident gallstones and the metabolic syndrome share common risk....... Gallstone incidence was assessed through repeated ultrasound examinations. Body mass index (BMI), blood pressure, self-rated health, lifestyle variables, blood lipids, and use of female sex hormones were measured at the baseline examination. Statistical analyses included logistic regression. Based...

  18. Formate-assisted pyrolysis (United States)

    DeSisto, William Joseph; Wheeler, Marshall Clayton; van Heiningen, Adriaan R. P.


    The present invention provides, among other thing, methods for creating significantly deoxygenated bio-oils form biomass including the steps of providing a feedstock, associating the feedstock with an alkali formate to form a treated feedstock, dewatering the treated feedstock, heating the dewatered treated feedstock to form a vapor product, and condensing the vapor product to form a pyrolysis oil, wherein the pyrolysis oil contains less than 30% oxygen by weight.

  19. Mars’ peculiar formation (United States)

    Woo, Man Yin; Brasser, Ramon; Mojzsis, Stephen; Matsumura, Soko; Ida, Shigeru


    The formation of the terrestrial planets is a long standing problem. Mars probably holds the key to solving this mystery because of the substantial amount of data gathered from space missions, martian meteorites and its obvious differences when compared to Earth. Recent elemental and isotopic abundances suggest that Mars’ composition is significantly different from that of Earth. Therefore, Mars should have accreted most of its mass in a region different from Earth’s, most likely further from the Sun. These compositional differences should be explained with planet formation models. We tested the probability of producing a Mars analogue that is compositionally different from Earth in two popular planet formation models: the Grand Tack model with tack locations of 1.5 or 2 AU for Jupiter; and the Classical model in which all the terrestrial planets formed near their current locations. We performed a high number of N-body simulations with initial conditions that are either equal-mass planetary embryos or a semi-analytical approach to oligarch growth. Our results show that the probability of producing a Mars analogue which matches the current mass and orbit of Mars is at most 9% but reduces to mostly about 1% when it mainly accretes its mass further than Mars’ current position of 1.5 AU. Strangely enough, in the Grand Tack model the number of Mars analogues produced is independent of the initial number of planet embryos. Hence, we conclude that both planet formation models have difficulties to explain the observed compositional differences between Earth and Mars.

  20. Formation of stellar clusters (United States)

    Smilgys, Romas; Bonnell, Ian A.


    We investigate the triggering of star formation and the formation of stellar clusters in molecular clouds which form as the interstellar medium passes through spiral shocks. The spiral shock compresses gas into an ∼100 pc long main star formation ridge, where clusters form every 5-10 pc along the merger ridge. We use a gravitational potential-based cluster finding algorithm, which extracts individual clusters, calculates their physical properties and traces cluster evolution over multiple time-steps. Final cluster masses at the end of simulation range between 1000 and 30 000 M⊙ with their characteristic half-mass radii between 0.1 and 2 pc. These clusters form by gathering material from 10-20 pc size scales. Clusters also show a mass-specific angular momentum relation, where more massive clusters have larger specific angular momentum due to the larger size scales, and hence angular momentum from which they gather their mass. The evolution shows that more massive clusters experience hierarchical merging process, which increases stellar age spreads up to 2-3 Myr. Less massive clusters appear to grow by gathering nearby recently formed sinks, while more massive clusters with their large global gravitational potentials are increasing their mass growth from gas accretion.

  1. Terrestrial planet formation. (United States)

    Righter, K; O'Brien, D P


    Advances in our understanding of terrestrial planet formation have come from a multidisciplinary approach. Studies of the ages and compositions of primitive meteorites with compositions similar to the Sun have helped to constrain the nature of the building blocks of planets. This information helps to guide numerical models for the three stages of planet formation from dust to planetesimals (~10(6) y), followed by planetesimals to embryos (lunar to Mars-sized objects; few 10(6) y), and finally embryos to planets (10(7)-10(8) y). Defining the role of turbulence in the early nebula is a key to understanding the growth of solids larger than meter size. The initiation of runaway growth of embryos from planetesimals ultimately leads to the growth of large terrestrial planets via large impacts. Dynamical models can produce inner Solar System configurations that closely resemble our Solar System, especially when the orbital effects of large planets (Jupiter and Saturn) and damping mechanisms, such as gas drag, are included. Experimental studies of terrestrial planet interiors provide additional constraints on the conditions of differentiation and, therefore, origin. A more complete understanding of terrestrial planet formation might be possible via a combination of chemical and physical modeling, as well as obtaining samples and new geophysical data from other planets (Venus, Mars, or Mercury) and asteroids.

  2. Formatting Design Dialogues

    DEFF Research Database (Denmark)

    Brandt, Eva; Binder, Thomas; Messeter, Jörn


    This article discusses design games as a particular genre for formatting design dialogues. In the first part of the article we review the participatory design literature for game-oriented framings of co-design. We look at what constitutes game and play, we discuss other authors’ use of games...... characteristics in being both ‘as-if’ worlds to explore and shared representations of what the players accomplish. In the last section of the article we discuss how new games may be designed and played and what makes a good design game....

  3. A role for the WH-30 protein in sperm-sperm adhesion during rouleaux formation in the guinea pig. (United States)

    Flaherty, S P; Swann, N J; Primakoff, P; Myles, D G


    Mammalian spermatozoa participate in specific cell adhesion phenomena during their development and functional lifespan; this includes interaction with Sertoli cells, the zona pellucida, and the oolemma. In some species such as the guinea pig, an additional sperm-sperm adhesion occurs during epididymal maturation which results in the formation of rouleaux in which the sperm heads are stacked one upon the other and the periacrosomal plasma membranes of adjacent sperm are linked by periodic cross-bridges. In this study, we have used a monoclonal antibody to investigate the role of the WH-30 protein on the sperm surface in the formation of the junctional zones between adjacent guinea pig sperm in rouleaux. WH-30 monoclonal antibodies caused a dose- and time-dependent dissociation of rouleaux and an increase in the percentage of single, acrosome-intact sperm; there were no effects on sperm motility (maintained at 80-90%) or ultrastructure during the 120-min incubations. The maximal effect of about 80% single sperm was obtained with a 1:4 dilution of the WH-30 hybridoma supernatant or 5-50 micrograms/ml of purified WH-30 IgG. In contrast, incubation of sperm in AH-20 IgG, myeloma cell supernatants, or purified, nonspecific mouse IgG1 had no effect on rouleaux. Treatment of sperm with a WH-30 Fab fragment resulted in almost complete dissociation of rouleaux without any observed effect on sperm motility or acrosomal status. Surface labeling of sperm followed by immunoprecipitation and SDS-PAGE revealed that the WH-30 antibody recognizes a single polypeptide of 43-45 kDa. Using immunofluorescence, the WH-30 protein was localized over the entire surface of the sperm head (whole-head pattern), and immunogold labeling showed that WH-30 is localized in the glycocalyx on both the dorsal and ventral surfaces of the periacrosomal and postacrosomal plasma membranes. These results indicate that the WH-30 protein on the sperm surface is a cell adhesion protein which is involved in

  4. Endocytosis and Enamel Formation

    Directory of Open Access Journals (Sweden)

    Cong-Dat Pham


    Full Text Available Enamel formation requires consecutive stages of development to achieve its characteristic extreme mineral hardness. Mineralization depends on the initial presence then removal of degraded enamel proteins from the matrix via endocytosis. The ameloblast membrane resides at the interface between matrix and cell. Enamel formation is controlled by ameloblasts that produce enamel in stages to build the enamel layer (secretory stage and to reach final mineralization (maturation stage. Each stage has specific functional requirements for the ameloblasts. Ameloblasts adopt different cell morphologies during each stage. Protein trafficking including the secretion and endocytosis of enamel proteins is a fundamental task in ameloblasts. The sites of internalization of enamel proteins on the ameloblast membrane are specific for every stage. In this review, an overview of endocytosis and trafficking of vesicles in ameloblasts is presented. The pathways for internalization and routing of vesicles are described. Endocytosis is proposed as a mechanism to remove debris of degraded enamel protein and to obtain feedback from the matrix on the status of the maturing enamel.

  5. Endocytosis and Enamel Formation (United States)

    Pham, Cong-Dat; Smith, Charles E.; Hu, Yuanyuan; Hu, Jan C-C.; Simmer, James P.; Chun, Yong-Hee P.


    Enamel formation requires consecutive stages of development to achieve its characteristic extreme mineral hardness. Mineralization depends on the initial presence then removal of degraded enamel proteins from the matrix via endocytosis. The ameloblast membrane resides at the interface between matrix and cell. Enamel formation is controlled by ameloblasts that produce enamel in stages to build the enamel layer (secretory stage) and to reach final mineralization (maturation stage). Each stage has specific functional requirements for the ameloblasts. Ameloblasts adopt different cell morphologies during each stage. Protein trafficking including the secretion and endocytosis of enamel proteins is a fundamental task in ameloblasts. The sites of internalization of enamel proteins on the ameloblast membrane are specific for every stage. In this review, an overview of endocytosis and trafficking of vesicles in ameloblasts is presented. The pathways for internalization and routing of vesicles are described. Endocytosis is proposed as a mechanism to remove debris of degraded enamel protein and to obtain feedback from the matrix on the status of the maturing enamel. PMID:28824442

  6. The Planet Formation Imager (United States)

    Kraus, S.; Buscher, D. F.; Monnier, J. D.; PFI Science, the; Technical Working Group


    Among the most fascinating and hotly-debated areas in contemporary astrophysics are the means by which planetary systems are assembled from the large rotating disks of gas and dust which attend a stellar birth. Although important work is being done both in theory and observation, a full understanding of the physics of planet formation can only be achieved by opening observational windows able to directly witness the process in action. The key requirement is then to probe planet-forming systems at the natural spatial scales over which material is being assembled. By definition, this is the so-called Hill Sphere which delineates the region of influence of a gravitating body within its surrounding environment. The Planet Formation Imager project has crystallized around this challenging goal: to deliver resolved images of Hill-Sphere-sized structures within candidate planet-hosting disks in the nearest star-forming regions. In this contribution we outline the primary science case of PFI and discuss how PFI could significantly advance our understanding of the architecture and potential habitability of planetary systems. We present radiation-hydrodynamics simulations from which we derive preliminary specifications that guide the design of the facility. Finally, we give an overview about the interferometric and non-interferometric technologies that we are investigating in order to meet the specifications.

  7. Laparoscopic Stoma Formation (United States)

    Tejirian, Talar


    Background: Laparoscopic stoma formation has gained wide acceptance as an alternative to open abdominal surgery. Although laparoscopic stoma formation has a low morbidity, complications have been reported. Contributing factors to these complications are twisting of the bowel, maturing the wrong limb, or both of these. In this report, we describe a simple technique that can reduce these complications. Methods: The bowel segment to be exteriorized is grasped with a locking nontraumatic, nonrotating grasper. After the orientation of the bowel is verified, the surgeon ties the handle of the instrument to the trocar by using a cotton umbilical tape. The trocar and the instrument become one working unit, and if the umbilical tape is wrapped around the shaft of the instrument, then the bowel is twisted. It is easy to untwist it by aligning the umbilical tape with the shaft of the instrument. To mature the stoma, the umbilical tape is removed and the grasper is unlocked. Conclusion: Laparoscopic stoma is an effective treatment for several benign and malignant disorders, and in general has a low morbidity. Our report describes a simple technique that can reduce the rare but significant postoperative stoma or small bowel obstruction. PMID:18435889

  8. FRS Geospatial Return File Format (United States)

    The Geospatial Return File Format describes format that needs to be used to submit latitude and longitude coordinates for use in Envirofacts mapping applications. These coordinates are stored in the Geospatail Reference Tables.

  9. Designing for informed group formation

    DEFF Research Database (Denmark)

    Nicolajsen, Hanne Westh; Juel Jacobsen, Alice; Riis, Marianne


    A new design ―project preparation‖ preparing for the group formation in problem based project work is proposed and investigated. The main problem is to overcome group formation based on existing relations. The hypothesis is that theme development and group formation are somewhat counterproductive...

  10. Group Formation in Economics (United States)

    Demange, Gabrielle; Wooders, Myrna


    Broad and diverse ranges of activities are conducted within and by organized groups of individuals, including political, economic and social activities. These activities have recently become a subject of intense interest in economics and game theory. Some of the topics investigated in this collection are models of networks of power and privilege, trade networks, co-authorship networks, buyer-seller networks with differentiated products, and networks of medical innovation and the adaptation of new information. Other topics are social norms on punctuality, clubs and the provision of club goods and public goods, research and development and collusive alliances among corporations, and international alliances and trading agreements. While relatively recent, the literature on game theoretic studies of group formation in economics is already vast. This volume provides an introduction to this important literature on game-theoretic treatments of situations with networks, clubs, and coalitions, including some applications.

  11. Myxobacteria Fruiting Body Formation (United States)

    Jiang, Yi


    Myxobacteria are social bacteria that swarm and glide on surfaces, and feed cooperatively. When starved, tens of thousands of cells change their movement pattern from outward spreading to inward concentration; they form aggregates that become fruiting bodies, inside which cells differentiate into nonmotile, environmentally resistant spores. Traditionally, cell aggregation has been considered to imply chemotaxis, a long-range cell interaction mediated by diffusing chemicals. However, myxobacteria aggregation is the consequence of direct cell-contact interactions. I will review our recent efforts in modeling the fruiting body formation of Myxobacteria, using lattice gas cellular automata models that are based on local cell-cell contact signaling. These models have reproduced the individual phases in Myxobacteria development such as the rippling, streaming, early aggregation and the final sporulation; the models can be unified to simulate the whole developmental process of Myxobacteria.

  12. Pattern Formation in Materials (United States)

    Karma, Alain


    Pattern formation is ubiquitous in nature, from sand ripples formed by wind to the development of a complex biological organism with different organs and a central nervous system. In the realm of materials, patterns are formed invariably when matter is transformed between different solid, liquid or gaseous states far from thermodynamic equilibrium. Material failure is itself mediated by the propagation of cracks that form intricate patterns. Understanding how patterns form and evolve is key to design materials with desired properties and to optimize their performance and safety. This talk will discuss recent progress made to understand three distinct class of patterns including the highly branched snow-flake-like dendritic patterns formed during the solidification process, polycrystalline patterns shaped by grain boundaries, and crack patterns.

  13. Recipes for planet formation (United States)

    Meyer, Michael R.


    Anyone who has ever used baking soda instead of baking powder when trying to make a cake knows a simple truth: ingredients matter. The same is true for planet formation. Planets are made from the materials that coalesce in a rotating disk around young stars - essentially the "leftovers" from when the stars themselves formed through the gravitational collapse of rotating clouds of gas and dust. The planet-making disk should therefore initially have the same gas-to-dust ratio as the interstellar medium: about 100 to 1, by mass. Similarly, it seems logical that the elemental composition of the disk should match that of the star, reflecting the initial conditions at that particular spot in the galaxy.

  14. Large Format Radiographic Imaging

    Energy Technology Data Exchange (ETDEWEB)

    J. S. Rohrer; Lacey Stewart; M. D. Wilke; N. S. King; S. A Baker; Wilfred Lewis


    Radiographic imaging continues to be a key diagnostic in many areas at Los Alamos National Laboratory (LANL). Radiographic recording systems have taken on many form, from high repetition-rate, gated systems to film recording and storage phosphors. Some systems are designed for synchronization to an accelerator while others may be single shot or may record a frame sequence in a dynamic radiography experiment. While film recording remains a reliable standby in the radiographic community, there is growing interest in investigating electronic recording for many applications. The advantages of real time access to remote data acquisition are highly attractive. Cooled CCD camera systems are capable of providing greater sensitivity with improved signal-to-noise ratio. This paper begins with a review of performance characteristics of the Bechtel Nevada large format imaging system, a gated system capable of viewing scintillators up to 300 mm in diameter. We then examine configuration alternatives in lens coupled and fiber optically coupled electro-optical recording systems. Areas of investigation include tradeoffs between fiber optic and lens coupling, methods of image magnification, and spectral matching from scintillator to CCD camera. Key performance features discussed include field of view, resolution, sensitivity, dynamic range, and system noise characteristics.

  15. Formation feedback control of UAV flight (United States)

    Stegall, Stephen

    This thesis is a study of formation control with autonomous unmanned aerial vehicles using the formation as feedback. There is also an investigation of formation methods presenting insight into different algorithms for formations. A rigid formation is achieved using a proportional-derivative virtual structure with a formation feedback controller. There is an emphasis on stick controlled aerodynamics. The rigid formation is verified by a simulation of a longitudinal model. Formation control ideas are presented for rigid formations.

  16. What Determines Star Formation Rates? (United States)

    Evans, Neal John


    The relations between star formation and gas have received renewed attention. We combine studies on scales ranging from local (within 0.5 kpc) to distant galaxies to assess what factors contribute to star formation. These include studies of star forming regions in the Milky Way, the LMC, nearby galaxies with spatially resolved star formation, and integrated galaxy studies. We test whether total molecular gas or dense gas provides the best predictor of star formation rate. The star formation ``efficiency," defined as star formation rate divided by mass, spreads over a large range when the mass refers to molecular gas; the standard deviation of the log of the efficiency decreases by a factor of three when the mass of relatively dense molecular gas is used rather than the mass of all the molecular gas. We suggest ways to further develop the concept of "dense gas" to incorporate other factors, such as turbulence.

  17. Triglycerides and gallstone formation. (United States)

    Smelt, A H M


    Changes in bile acid (BA) metabolism and gallbladder function are critical factors in the pathogenesis of gallstones. Patients with hypertriglyceridemia (HTG) - often overweight and insulin resistant - are at risk for gallstone disease. The question arises whether HTG itself contributes to gallstone formation or whether gallstone disease only associates with this disorder. Triglycerides are formed in response to fluxes of non-esterified fatty acids and glucose. Hypertriglyceridemia results from either overproduction of triglycerides by the liver, impaired lipolysis or a combination of both. Hyperinsulinemia, as observed in the insulin resistant state, stimulates very low-density lipoprotein (VLDL)-triglyceride synthesis. Peroxisome proliferator-activated receptors (PPARs), liver X receptors (LXRs), farnesoid X receptor (FXR) and hepatocyte nuclear factor 4α (HNF4α) are the nuclear receptors involved in the regulation of lipogenesis. Microsomal triglyceride transfer protein (MTP) is involved in the production of VLDL and its activation is also under control of transcription factors as FXR and Forkhead box-O1 (FoxO1). Triglyceride and BA metabolism are linked. There is an inverse relationship between bile acid fluxes and pool size and VLDL production and SHP (small heterodimer partner) and FXR are the link between BAs and TG metabolism. BAs are also ligands for FXR and G-protein-coupled receptors, such as TGR5. FXR activation by BAs suppresses the expression of MTP, transcription factor sterol regulatory element binding protein (SREBP)-1c and other lipogenic genes. LXRs stimulate lipogenesis whereas FXRs inhibit the metabolic process. Synthesis of BAs from cholesterol occurs either via the classical pathway (7α-hydroxylation of cholesterol; CYP7A1) or via the alternate pathway (CYP39A1 or CYP7B1). BAs induce FXR, which inhibits CYP7A1 transcription by activation of SHP and inhibition of HNF4α transactivation. Bile composition (supersaturation with cholesterol

  18. Satellite formation flying relative dynamics, formation design, fuel optimal maneuvers and formation maintenance

    CERN Document Server

    Wang, Danwei; Poh, Eng Kee


    This book systematically describes the concepts and principles for multi-satellite relative motion, passive and near passive formation designs, trajectory planning and control for fuel optimal formation maneuvers, and formation flying maintenance control design. As such, it provides a sound foundation for researchers and engineers in this field to develop further theories and pursue their implementations. Though satellite formation flying is widely considered to be a major advance in space technology, there are few systematic treatments of the topic in the literature. Addressing that gap, the book offers a valuable resource for academics, researchers, postgraduate students and practitioners in the field of satellite science and engineering.

  19. Planet formation around millisecond pulsars (United States)

    Banit, Menashe; Ruderman, Malvin; Shaham, Jacob


    We present a model for the formation of planets in circular orbits around millisecond pulsars. We propose that the planets originate from a circumbinary excretion disk around a binary millisecond pulsar and show how physical conditions in such a disk lead to the eventual formation of planets.

  20. Bundle Formation in Biomimetic Hydrogels

    NARCIS (Netherlands)

    Jaspers, Maarten; Pape, A C H; Voets, Ilja K; Rowan, Alan E; Portale, Giuseppe; Kouwer, Paul H J


    Bundling of single polymer chains is a crucial process in the formation of biopolymer network gels that make up the extracellular matrix and the cytoskeleton. This bundled architecture leads to gels with distinctive properties, including a large-pore-size gel formation at very low concentrations and

  1. Pathogenesis of postoperative adhesion formation

    NARCIS (Netherlands)

    Hellebrekers, B.W.J.; Kooistra, T.


    Background: Current views on the pathogenesis of adhesion formation are based on the "classical concept of adhesion formation", namely that a reduction in peritoneal fibrinolytic activity following peritoneal trauma is of key importance in adhesion development. Methods: A non-systematic literature

  2. Tooth formation - delayed or absent (United States)

    ... tooth formation; Teeth - delayed or absent formation Images Tooth anatomy Development of baby teeth Development of permanent teeth References ... MO: Elsevier Mosby; 2016:chap 19. Tinanoff N. Development and developmental anomalies of the teeth. In: Kliegman RM, ... NIH MedlinePlus Magazine Read more ...

  3. Identity formation in multiparty negotiations

    NARCIS (Netherlands)

    Swaab, R; Postmes, T.; Spears, R.

    Based on the recently proposed Interactive Model of Identity Formation, we examine how top-down deductive and bottom-up inductive identity formations influence intentions and behaviour in multiparty negotiations. Results show that a shared identity can be deduced from the social context through

  4. Physics of primordial star formation (United States)

    Yoshida, Naoki


    The study of primordial star formation has a history of nearly sixty years. It is generally thought that primordial stars are one of the key elements in a broad range of topics in astronomy and cosmology, from Galactic chemical evolution to the formation of super-massive blackholes. We review recent progress in the theory of primordial star formation. The standard theory of cosmic structure formation posits that the present-day rich structure of the Universe developed through gravitational amplification of tiny matter density fluctuations left over from the Big Bang. It has become possible to study primordial star formation rigorously within the framework of the standard cosmological model. We first lay out the key physical processes in a primordial gas. Then, we introduce recent developments in computer simulations. Finally, we discuss prospects for future observations of the first generation of stars.

  5. Free-format RPG IV

    CERN Document Server

    Martin, Jim


    This how-to guide offers a concise and thorough introduction to the increased productivity, better readability, and easier program maintenance that comes with the free-format style of programming in RPG IV. Although free-format information is available in IBM manuals, it is not separated from everything else, thereby requiring hours of tedious research to track down the information needed. This book provides everything one needs to know to write RPG IV in the free-format style, and author Jim Martin not only teaches rules and syntax but also explains how this new style of coding has the pot

  6. Giant Planet Formation and Migration (United States)

    Paardekooper, Sijme-Jan; Johansen, Anders


    Planets form in circumstellar discs around young stars. Starting with sub-micron sized dust particles, giant planet formation is all about growing 14 orders of magnitude in size. It has become increasingly clear over the past decades that during all stages of giant planet formation, the building blocks are extremely mobile and can change their semimajor axis by substantial amounts. In this chapter, we aim to give a basic overview of the physical processes thought to govern giant planet formation and migration, and to highlight possible links to water delivery.

  7. Magnetic Fields And Star Formation (United States)

    Zhang, Qizhou


    Magnetic fields can have a significant effect on the formation and evolution of molecular clouds and the formation of stars. The presence of strong magnetic fields restricts the motion of gas along the magnetic field lines. Therefore, it resists gravitational collapse, hinders mass accretion and suppresses fragmentation. While magnetic fields are an integral part of modern theory of interstellar medium and star formation, their direct measurements have been challenging. In this talk, I'll review recent progress on the observational front of magnetic fields. The emphasis will be on linear polarization of interstellar dust to probe the plane of sky component of magnetic fields.

  8. Age differences in concept formation. (United States)

    Hartman, Marilyn; Stratton-Salib, Brea C


    In order to identify the source of age-related deficits in concept formation, older and younger adults completed a new Concept Matching Test, tests of selective attention, and the Sorting Test, a standardized test of concept formation (Delis, Kaplan, & Kramer, 2001). Older adults showed intact concept identification, the ability to generate abstract concepts from stimulus features, but age differences were observed when irrelevant features were present. In addition, selective attention, but not concept identification ability, predicted age-related declines on the Sorting Test. Thus, reduced concept formation in older adults may stem from declines in abilities other than the capacity for abstraction.

  9. AGN feedback in galaxy formation

    CERN Document Server

    Antonuccio-Delogu, Vincenzo


    During the past decade, convincing evidence has been accumulated concerning the effect of active galactic nuclei (AGN) activity on the internal and external environment of their host galaxies. Featuring contributions from well-respected researchers in the field, and bringing together work by specialists in both galaxy formation and AGN, this volume addresses a number of key questions about AGN feedback in the context of galaxy formation. The topics covered include downsizing and star-formation time scales in massive elliptical galaxies, the connection between the epochs of supermassive black h

  10. Positronium formation from C60 (United States)

    Hervieux, Paul-Antoine; Chakraborty, Anzumaan; Chakraborty, Himadri


    Due to the dominant electron capture by positrons from the molecular shell and the spatial dephasing across the shell-width, a powerful diffraction effect universally underlies the positronium (Ps) formation from C60. This results into trains of resonances in the Ps formation cross section as a function of the positron beam energy, producing structures in recoil momenta in analogy with classical single-slit diffraction fringes in the configuration space. C60 is modeled by a jellium-based local-density approximation (LDA) method and the Ps formation is treated by the continuum distorted-wave final-state (CDW-FS) approximation. The work may motivate application of the Ps formation spectroscopy to gas-phase nanoparticles and also the access target-level- as well as Ps-level-differential measurements. This work was supported by the U.S. National Science Foundation.

  11. Formation of Rationally Heterogeneous Expectations

    NARCIS (Netherlands)

    Pfajfar, D.


    Abstract: This paper models expectation formation by taking into account that agents produce heterogeneous expectations due to model uncertainty, informational frictions and different capacities for processing information. We show that there are two general classes of steady states within this

  12. Modularity in New Market Formation

    DEFF Research Database (Denmark)

    Sanchez, Ron; Hang, Chang Chieh


    the formation of an industrial base of assemblers and component suppliers, assisting new firms in building customer relationships, enabling more geographically diffused economic development within countries, and facilitating development of export markets. We also suggest directions for further research......In this paper we appraise the ways in which use of closed-system proprietary product architectures versus open-system modular product architectures is likely to influence the dynamics and trajectory of new product market formation. We compare the evolutions of new markets in China for gas...... as the basis for new kinds of products may result in very different patterns and speeds of new market formation. We then suggest some key implications of the different dynamics of market formation associated with open-system modular architectures for both the competence-based strategic management (CBSM...

  13. Motion Predicts Clinical Callus Formation (United States)

    Elkins, Jacob; Marsh, J. Lawrence; Lujan, Trevor; Peindl, Richard; Kellam, James; Anderson, Donald D.; Lack, William


    Background: Mechanotransduction is theorized to influence fracture-healing, but optimal fracture-site motion is poorly defined. We hypothesized that three-dimensional (3-D) fracture-site motion as estimated by finite element (FE) analysis would influence callus formation for a clinical series of supracondylar femoral fractures treated with locking-plate fixation. Methods: Construct-specific FE modeling simulated 3-D fracture-site motion for sixty-six supracondylar femoral fractures (OTA/AO classification of 33A or 33C) treated at a single institution. Construct stiffness and directional motion through the fracture were investigated to assess the validity of construct stiffness as a surrogate measure of 3-D motion at the fracture site. Callus formation was assessed radiographically for all patients at six, twelve, and twenty-four weeks postoperatively. Univariate and multivariate linear regression analyses examined the effects of longitudinal motion, shear (transverse motion), open fracture, smoking, and diabetes on callus formation. Construct types were compared to determine whether their 3-D motion profile was associated with callus formation. Results: Shear disproportionately increased relative to longitudinal motion with increasing bridge span, which was not predicted by our assessment of construct stiffness alone. Callus formation was not associated with open fracture, smoking, or diabetes at six, twelve, or twenty-four weeks. However, callus formation was associated with 3-D fracture-site motion at twelve and twenty-four weeks. Longitudinal motion promoted callus formation at twelve and twenty-four weeks (p = 0.017 for both). Shear inhibited callus formation at twelve and twenty-four weeks (p = 0.017 and p = 0.022, respectively). Titanium constructs with a short bridge span demonstrated greater longitudinal motion with less shear than did the other constructs, and this was associated with greater callus formation (p callus formation, while shear inhibited

  14. Chain formation of metal atoms

    DEFF Research Database (Denmark)

    Bahn, Sune Rastad; Jacobsen, Karsten Wedel


    The possibility of formation of single-atomic chains by manipulation of nanocontacts is studied for a selection of metals (Ni, Pd, Pt, Cu, Ag, Au). Molecular dynamics simulations show that the tendency for chain formation is strongest for Au and Pt. Density functional theory calculations indicate...... that the metals which form chains exhibit pronounced many-atom interactions with strong bonding in low coordinated systems....

  15. Problem of professional personality formation

    Directory of Open Access Journals (Sweden)

    Pryazhnikova E.Yu.


    Full Text Available The article presents the current approaches to the problem of professional personality formation. It ana-lyzes the impact of professional identity of students on the choice of the specialist field they make. The article highlights certain aspects of the educational environment which promote to formation of compe-tences in students, relating to their choice of professional occupations. The article presents the analysis of students’ internal and external strivings after graduation from the university and their fulfillment.

  16. Modeling bromate formation during ozonation


    JARVIS, Peter; Parsons, Simon A.; Smith, Rosie


    Bromate formation has been identified as a significant barrier in the application of ozone during water treatment for water sources that contain high levels of bromide. Bromate has been identified as a possible human carcinogen and bromate levels in drinking water are strictly controlled at 10 mu g/L in most developed countries. Various models have been proposed to model bromate formation during ozonation based on raw water quality, ozone dose and contact time. Two main appr...

  17. Microtiter dish biofilm formation assay. (United States)

    O'Toole, George A


    Biofilms are communities of microbes attached to surfaces, which can be found in medical, industrial and natural settings. In fact, life in a biofilm probably represents the predominate mode of growth for microbes in most environments. Mature biofilms have a few distinct characteristics. Biofilm microbes are typically surrounded by an extracellular matrix that provides structure and protection to the community. Microbes growing in a biofilm also have a characteristic architecture generally comprised of macrocolonies (containing thousands of cells) surrounded by fluid-filled channels. Biofilm-grown microbes are also notorious for their resistance to a range of antimicrobial agents including clinically relevant antibiotics. The microtiter dish assay is an important tool for the study of the early stages in biofilm formation, and has been applied primarily for the study of bacterial biofilms, although this assay has also been used to study fungal biofilm formation. Because this assay uses static, batch-growth conditions, it does not allow for the formation of the mature biofilms typically associated with flow cell systems. However, the assay has been effective at identifying many factors required for initiation of biofilm formation (i.e, flagella, pili, adhesins, enzymes involved in cyclic-di-GMP binding and metabolism) and well as genes involved in extracellular polysaccharide production. Furthermore, published work indicates that biofilms grown in microtiter dishes do develop some properties of mature biofilms, such a antibiotic tolerance and resistance to immune system effectors. This simple microtiter dish assay allows for the formation of a biofilm on the wall and/or bottom of a microtiter dish. The high throughput nature of the assay makes it useful for genetic screens, as well as testing biofilm formation by multiple strains under various growth conditions. Variants of this assay have been used to assess early biofilm formation for a wide variety of microbes

  18. Star Formation Rate Indicators in the FIRE Galaxy Formation Simulations (United States)

    Flores, Jose Antonio; Gurvich, Alex; Faucher-Giguère, Claude-André; Sparre, Martin; Hayward, Christopher; Feedback In Realistic Environments (FIRE) Collaboration


    Understanding the rate at which stars form is vital to understanding galaxy formation. Observationally, the star formation rates (SFRs) of galaxies are typically measured using light in different bands under the assumption of a time-steady SFR. We use galaxy formation simulations from the Feedback In Realistic Environments (FIRE) project, which in some regimes predict (time variable) bursty SFRs, to analyze the timescales probed by H-alpha and far ultraviolet (FUV) SFR indicators. We also quantify the possible dependence of SFR indicators on SFR variability. Our preliminary results based on a Milky Way-mass galaxy simulation indicate that the best-fit timescales probed by these indicators do not depend significantly on whether the SFR is bursty, with best-fitting timescales of about 4 Myr for H-alpha and about 10 Myr for FUV in both the time-steady and bursty regimes.

  19. An Adaptable Seismic Data Format (United States)

    Krischer, Lion; Smith, James; Lei, Wenjie; Lefebvre, Matthieu; Ruan, Youyi; de Andrade, Elliott Sales; Podhorszki, Norbert; Bozdağ, Ebru; Tromp, Jeroen


    We present ASDF, the Adaptable Seismic Data Format, a modern and practical data format for all branches of seismology and beyond. The growing volume of freely available data coupled with ever expanding computational power opens avenues to tackle larger and more complex problems. Current bottlenecks include inefficient resource usage and insufficient data organization. Properly scaling a problem requires the resolution of both these challenges, and existing data formats are no longer up to the task. ASDF stores any number of synthetic, processed or unaltered waveforms in a single file. A key improvement compared to existing formats is the inclusion of comprehensive meta information, such as event or station information, in the same file. Additionally, it is also usable for any non-waveform data, for example, cross-correlations, adjoint sources or receiver functions. Last but not least, full provenance information can be stored alongside each item of data, thereby enhancing reproducibility and accountability. Any data set in our proposed format is self-describing and can be readily exchanged with others, facilitating collaboration. The utilization of the HDF5 container format grants efficient and parallel I/O operations, integrated compression algorithms and check sums to guard against data corruption. To not reinvent the wheel and to build upon past developments, we use existing standards like QuakeML, StationXML, W3C PROV and HDF5 wherever feasible. Usability and tool support are crucial for any new format to gain acceptance. We developed mature C/Fortran and Python based APIs coupling ASDF to the widely used SPECFEM3D_GLOBE and ObsPy toolkits.

  20. Pyruvate Formate-Lyase Interacts Directly with the Formate Channel FocA to Regulate Formate Translocation


    Doberenz, Claudia; Zorn, Michael; Falke, Dörte; Nannemann, David; Hunger, Doreen; Beyer, Lydia; Christian H Ihling; Meiler, Jens; Sinz, Andrea; Sawers, R. Gary


    The FNT (formate-nitrite transporters) form a superfamily of pentameric membrane channels that translocate monovalent anions across biological membranes. FocA (formate channel A) translocates formate bidirectionally but the mechanism underlying how translocation of formate is controlled and what governs substrate specificity remains unclear. Here we demonstrate that the normally soluble dimeric enzyme pyruvate formate-lyase (PflB), which is responsible for intracellular formate generation in ...

  1. Effects of ammonium ion removal on growth and MAb production of hybridoma cells. (United States)

    Matsumura, M; Nayve, F R


    Production of monoclonal antibody against hepatitis B surface antigen was carried out by perfusion culture coupled with a selective removal system for ammonium ion. The removal system is composed of three sub-systems namely, cell separation by cross-flow ceramic filter, dialysis by hollow fiber module and ion-exchange by zeolite A-3 packed bed column. The ammonium ion concentration in the culture broth was effectively maintained below the inhibitory level, and the viable cell density reached 2.5×10(7) cells ml(-1) which was three times that of conventional perfusion cultures. The monoclonal antibody accumulated to a concentration as high as 26.3×10(5) mIU(-1). This is already almost half of the amount producedin vivo. The numerical investigation of the ammonium ion removal system showed the possibility to improve much more the performance of this perfusion cultivation system.

  2. Evaluation of Hybridoma B9 cell line handling of Cadmium in the ...

    African Journals Online (AJOL)

    6 (IL-6) was one of the responses examined to identify the mechanism by which IL-6 (at high concentration) was able to reverse cadmium inhibition of B9 cell line proliferation. Cellular metallothionein was assayed by Cd-haem method.

  3. A novel hybridoma antibody (PASE/4LJ) to human prostatic acid phosphatase suitable for immunohistochemistry

    National Research Council Canada - National Science Library

    Haines, A M; Larkin, S E; Richardson, A P; Stirling, R W; Heyderman, E


    ... in a pathological fracture of lymph node is of pros- tatic origin, or whether an adenocarcinoma in the wall of the rectum without obvious mucosal involvement is an invading carcinoma from the prostate, or is of primary rectal origin. Similarly, deciding whether tumours arising in the region of the bladder neck are of bladder or prostatic origin m...

  4. Acoustic perfusion processes for hybridoma cultures: viability, cell cycle and metabolic analysis

    NARCIS (Netherlands)

    Dalm, M.C.F.


    For the production of glycosylated proteins, such as monoclonal antibodies, hormones, and blood clothing factors, generally mammalian cells are used. Mammalian cells are preferred over other expression systems, such as bacteria or yeast, because they are able to glycosylate proteins in a human-like

  5. The Physics of Planetesimal Formation (United States)

    Simon, Jacob; Armitage, Philip; Youdin, Andrew; Li, Rixin


    Planetesimals are the precursors to planets, and understanding their formation is an essential step towards developing a complete theory of planet formation. For small solid particles (e.g., dust grains) to coagulate into planetesimals, however, requires that these particles grow beyond centimeter sizes; with traditional coagulation physics, this is very difficult. The streaming instability, which is a clumping process akin to the pile-up of cars in a traffic jam, generates sufficiently high solid densities that the mutual gravity between the clumped particles eventually causes their collapse towards planetesimal mass and size scales. Exploring this transition from dust grains to planetesimals is still in its infancy but is extremely important if we want to understand the basics of planet formation. Here, I present a series of high resolution, first principles numerical simulations of potoplanetary disk gas and dust to study the clumping of particles via the streaming instability and the subsequent collapse towards planetesimals. These simulations have been employed to characterize the planetesimal population as a function of radius in protoplanetary disks. The results of these simulations will be crucial for planet formation models to correctly explain the formation and configuration of solar systems.

  6. Exoplanets Detection, Formation, Properties, Habitability

    CERN Document Server

    Mason, John W


    This edited, multi-author volume will be an invaluable introduction and reference to all key aspects in the field of exoplanet research. The reviews cover: Detection methods and properties of known exoplanets, Detection of extrasolar planets by gravitational microlensing. The formation and evolution of terrestrial planets in protoplanetary and debris disks. The brown dwarf-exoplanet connection. Formation, migration mechanisms and properties of hot Jupiters. Dynamics of multiple exoplanet systems. Doppler exoplanet surveys. Searching for exoplanets in the stellar graveyard. Formation and habitability of extra solar planets in multiple star systems. Exoplanet habitats and the possibilities for life. Moons of exoplanets: habitats for life. Contributing authors: •Rory Barnes •David P. Bennett •Jian Ge •Nader Haghighipour •Patrick Irwin •Hugh Jones •Victoria Meadows •Stanimir Metchev •I. Neill Reid •George Rieke •Caleb Scharf •Steinn Sigurdsson

  7. Structure formation in active networks

    CERN Document Server

    Köhler, Simone; Bausch, Andreas R


    Structure formation and constant reorganization of the actin cytoskeleton are key requirements for the function of living cells. Here we show that a minimal reconstituted system consisting of actin filaments, crosslinking molecules and molecular-motor filaments exhibits a generic mechanism of structure formation, characterized by a broad distribution of cluster sizes. We demonstrate that the growth of the structures depends on the intricate balance between crosslinker-induced stabilization and simultaneous destabilization by molecular motors, a mechanism analogous to nucleation and growth in passive systems. We also show that the intricate interplay between force generation, coarsening and connectivity is responsible for the highly dynamic process of structure formation in this heterogeneous active gel, and that these competing mechanisms result in anomalous transport, reminiscent of intracellular dynamics.

  8. Black holes and galaxy formation

    CERN Document Server

    Propst, Raphael J


    Galaxies are the basic unit of cosmology. The study of galaxy formation is concerned with the processes that formed a heterogeneous universe from a homogeneous beginning. The physics of galaxy formation is complicated because it deals with the dynamics of stars, thermodynamics of gas and energy production of stars. A black hole is a massive object whose gravitational field is so intense that it prevents any form of matter or radiation to escape. It is hypothesized that the most massive galaxies in the universe- "elliptical galaxies"- grow simultaneously with the supermassive black holes at their centers, giving us much stronger evidence that black holes control galaxy formation. This book reviews new evidence in the field.

  9. Granuloma formation in pulmonary sarcoidosis

    Directory of Open Access Journals (Sweden)

    Caroline E. Broos


    Full Text Available Sarcoidosis is a granulomatous disorder of unknown cause, affecting multiple organs, but mainly the lungs. The exact order of immunological events remains obscure. Reviewing current literature, combined with careful clinical observations, we propose a model for granuloma formation in pulmonary sarcoidosis. A tight collaboration between macrophages, dendritic cells and lymphocyte subsets, initiates the first steps towards granuloma formation, orchestrated by cytokines and chemokines. In a substantial part of pulmonary sarcoidosis patients, granuloma formation becomes an on-going process, leading to debilitating disease and sometimes death. The immunological response, determining granuloma sustainment is not well understood. An impaired immunosuppressive function of regulatory T cells has been suggested to contribute to the exaggerated response. Interestingly, therapeutical agents commonly used in sarcoidosis, such as glucocorticosteroids and anti-TNF agents, interfere with granuloma integrity and restore the immune homeostasis in autoimmune disorders. Increasing insight into their mechanisms of action may contribute to the search for new therapeutical targets in pulmonary sarcoidosis.

  10. Formation Flying In Highly Elliptical Orbits Initializing the Formation (United States)

    Mailhe, Laurie; Schiff, Conrad; Hughes, Steven


    In this paper several methods are examined for initializing formations in which all spacecraft start in a common elliptical orbit subsequent to separation from the launch vehicle. The tetrahedron formation used on missions such as the Magnetospheric Multiscale (MMS), Auroral Multiscale Midex (AMM), and Cluster is used as a test bed Such a formation provides full three degrees-of-freedom in the relative motion about the reference orbit and is germane to several missions. The type of maneuver strategy that can be employed depends on the specific initial conditions of each member of the formation. Single-impulse maneuvers based on a Gaussian variation-of-parameters (VOP) approach, while operationally simple and intuitively-based, work only in a limited sense for a special class of initial conditions. These 'tailored' initial conditions are characterized as having only a few of the Keplerian elements different from the reference orbit. Attempts to achieve more generic initial conditions exceed the capabilities of the single impulse VOP. For these cases, multiple-impulse implementations are always possible but are generally less intuitive than the single-impulse case. The four-impulse VOP formalism discussed by Schaub is examined but smaller delta-V costs are achieved in our test problem by optimizing a Lambert solution.

  11. Regulation of Reactionary Dentine Formation. (United States)

    Neves, V C M; Sharpe, P T


    During the treatment of dental caries that has not penetrated the tooth pulp, maintenance of as much unaffected dentine as possible is a major goal during the physical removal of decayed mineral. Damage to dentine leads to release of fossilized factors (transforming growth factor-β [TGF-β] and bone morphogenic protein [BMP]) in the dentine that are believed to stimulate odontoblasts to secrete new "tertiary" dentine (reactionary dentine). This is formed on the pulpal surface of existing dentine and rethickens the dentine. We have previously shown that activation of Wnt/β-catenin signaling is pivotal for tooth repair in exposed pulp injury, and the pathway can be activated by small-molecule GSK-3 antagonists, resulting in enhanced reparative dentine formation. Here, we use a nonexposed pulp injury model to investigate the mechanisms of reactionary dentine formation in vivo, using small molecules to modulate the Wnt/β-catenin, TGF-β, and BMP pathways. We found that a local increase of Wnt activation at the injury site enhances reactionary dentine secretion. In addition, inhibition of TGF-β, BMP, or Wnt pathways does not impede reactionary dentine formation, although inhibition of TGF-β and/or BMP signaling does result in more disorganized, nontubular reactionary dentine. This suggests that Wnt/β-catenin signaling plays no major role in the formation of reactionary dentine, but in common with reparative dentine formation, exogenous elevation of Wnt/β-catenin signaling can enhance tertiary dentine formation. Release of latent TGF-β or BMPs from dentine is not required for the deposition of mineral to form reactionary dentine but does play a role in its organization.

  12. Pattern formations and oscillatory phenomena

    CERN Document Server

    Kinoshita, Shuichi


    Patterns and their formations appear throughout nature, and are studied to analyze different problems in science and make predictions across a wide range of disciplines including biology, physics, mathematics, chemistry, material science, and nanoscience. With the emergence of nanoscience and the ability for researchers and scientists to study living systems at the biological level, pattern formation research has become even more essential. This book is an accessible first of its kind guide for scientists, researchers, engineers, and students who require a general introduction to thi

  13. Pattern formation in the geosciences. (United States)

    Goehring, Lucas


    Pattern formation is a natural property of nonlinear and non-equilibrium dynamical systems. Geophysical examples of such systems span practically all observable length scales, from rhythmic banding of chemical species within a single mineral crystal, to the morphology of cusps and spits along hundreds of kilometres of coastlines. This article briefly introduces the general principles of pattern formation and argues how they can be applied to open problems in the Earth sciences. Particular examples are then discussed, which summarize the contents of the rest of this Theme Issue.

  14. Terrestrial Planet Formation: Constraining the Formation of Mercury (United States)

    Lykawka, Patryk Sofia; Ito, Takashi


    How the four terrestrial planets of the solar system formed is one of the most fundamental questions in the planetary sciences. Particularly, the formation of Mercury remains poorly understood. We investigated terrestrial planet formation by performing 110 high-resolution N-body simulation runs using more than 100 embryos and 6000 disk planetesimals representing a primordial protoplanetary disk. To investigate the formation of Mercury, these simulations considered an inner region of the disk at 0.2-0.5 au (the Mercury region) and disks with and without mass enhancements beyond the ice line location, a IL, in the disk, where a IL = 1.5, 2.25, and 3.0 au were tested. Although Venus and Earth analogs (considering both orbits and masses) successfully formed in the majority of the runs, Mercury analogs were obtained in only nine runs. Mars analogs were also similarly scarce. Our Mercury analogs concentrated at orbits with a ˜ 0.27-0.34 au, relatively small eccentricities/inclinations, and median mass m ˜ 0.2 {M}\\oplus . In addition, we found that our Mercury analogs acquired most of their final masses from embryos/planetesimals initially located between 0.2 and ˜1-1.5 au within 10 Myr, while the remaining mass came from a wider region up to ˜3 au at later times. Although the ice line was negligible in the formation of planets located in the Mercury region, it enriched all terrestrial planets with water. Indeed, Mercury analogs showed a wide range of water mass fractions at the end of terrestrial planet formation.

  15. Formative Assessment in Dance Education (United States)

    Andrade, Heidi; Lui, Angela; Palma, Maria; Hefferen, Joanna


    Feedback is crucial to students' growth as dancers. When used within the framework of formative assessment, or assessment for learning, feedback results in actionable next steps that dancers can use to improve their performances. This article showcases the work of two dance specialists, one elementary and one middle school teacher, who have…

  16. The Road to Galaxy Formation

    CERN Document Server

    Keel, William C


    The formation of galaxies is one of the greatest puzzles in astronomy, the solution is shrouded in the depths of space and time, but has profound implications for the universe we observe today. The book discusses the beginnings of the process from cosmological observations and calculations, considers the broad features of galaxies that we need to explain and what we know of their later history. The author compares the competing theories for galaxy formation and considers the progress expected from new generations of powerful telescopes both on earth and in space. In this second edition the author has retained the observationally-based approach of the first edition, a feature which was particularly well-reviewed: Writing in Nature, Carlton Baugh noted in February 2003 that “It is refreshing, in a market dominated by theorists, to come across a book on galaxy formation written from an observational perspective. The Road to Galaxy Formation should prove to be a handy primer on observations for graduate student...

  17. Junction formation during desiccation cracking. (United States)

    Toga, K B; Alaca, B Erdem


    In order to provide a sound physical basis for the understanding of the formation of desiccation crack networks, an experimental study is presented addressing junction formation. Focusing on junctions, basic features of the network determining the final pattern, provides an elemental approach and imparts conceptual clarity to the rather complicated problem of the evolution of crack patterns. Using coffee-water mixtures a clear distinction between junction formation during nucleation and propagation is achieved. It is shown that for the same drying suspension, one can switch from the well-known symmetric triple junctions that are unique to the nucleation phase to propagation junctions that are purely dictated by the variations of the stress state. In the latter case, one can even manipulate the path of a propagating crack in a deterministic fashion by changing the stress state within the suspension. Clear microscopic evidence is provided for the formation of propagation junctions, and material inhomogeneity is observed to be reflected by a broad distribution of angles, in stark contrast to shrinkage cracks in homogeneous solid films.

  18. Shell formation and nuclear masses

    Energy Technology Data Exchange (ETDEWEB)

    Zuker, A. P. [IPHC, IN2P3-CNRS, Universite Louis Pasteur, F-67037 Strasbourg (France)]. e-mail:


    We describe the basic mechanisms responsible for nuclear bulk properties and shell formation incorporated in the Duflo Zuker models. The emphasis is put on explaining why functionals of the occupancies can be so efficient in accounting for data with minimal computational effort. (Author)

  19. The EPRDATA Format: A Dialogue

    Energy Technology Data Exchange (ETDEWEB)

    Hughes, Henry Grady III [Los Alamos National Laboratory


    Recently the Los Alamos Nuclear Data Team has communicated certain issues of concern in relation to the new electron/photon/relaxation ACE data format as released in the eprdata12 library. In this document those issues are parsed, analyzed, and answered.

  20. Playing Games with Formative Assessment (United States)

    Cassie, Jonathan


    Games can be great tools to engage reluctant learners and provide ongoing feedback to educators about how their lessons are "sticking." Cassie discusses how to use gamified formative assessments to measure different kinds of skills and looks at the different ways teachers can use games in the classroom--from out-of-the-box board games to…

  1. Exciton Formation in Disordered Semiconductors

    DEFF Research Database (Denmark)

    Klochikhin, A.; Reznitsky, A.; Permogorov, S.


    Stationary luminescence spectra of disordered solid solutions can be accounted by the model of localized excitons. Detailed analysis of the long time decay kinetics of luminescence shows that exciton formation in these systems is in great extent due to the bimolecular reaction of separated carrie...

  2. The Inclusion of Word Formation

    African Journals Online (AJOL)


    The Inclusion of Word Formation in OALD8: The Case of. Undefined Run-ons. Alenka Vrbinc (, Faculty of Economics, and. Marjeta Vrbinc (, Faculty of Arts. University of Ljubljana, Ljubljana, Slovenia. Abstract: The study presented in this contribution aims to investigate ...

  3. Genetic analysis of symbiosome formation

    NARCIS (Netherlands)

    Ovchinnikova, E.


    Endosymbiotic interactions form a fundament of life as we know it and are characterized by the formation of new specialized membrane compartments, in which the microbes are hosted inside living plant cells. A striking example is the symbiosis between legumes and nitrogen-fixing Rhizobium bacteria

  4. Adventitious root formation in Arabidopsis

    NARCIS (Netherlands)

    Massoumi, Mehdi


    Adventitious root (AR) formation is indispensable in vegetative propagation and is widely used. A better understanding of the underlying mechanisms is needed to improve rooting treatments. We first established a system to study rooting in Arabidopsis, the model organism in plant biology but only

  5. Granuloma formation in pulmonary sarcoidosis

    NARCIS (Netherlands)

    C.E. Broos (Caroline); M. van Nimwegen (Menno); H.C. Hoogsteden (Henk); R.W. Hendriks (Rudi); M. Kool (Mirjam); B. van den Blink (Bernt)


    textabstractSarcoidosis is a granulomatous disorder of unknown cause, affecting multiple organs, but mainly the lungs. The exact order of immunological events remains obscure. Reviewing current literature, combined with careful clinical observations, we propose a model for granuloma formation in

  6. Physicochemical mechanisms of stone formation. (United States)

    Rodgers, Allen L


    In this article, the term "physicochemical mechanism" is defined as a sequential series of steps culminating in the formation of a renal stone. Distinctions are drawn between physicochemical prerequisites for urinary supersaturation, crystallization, and stone formation. In particular, attention is focussed on the transition from crystal to stone. Emphasis is laid on crystal retention being the fundamental mechanism by which stones are formed, and mention is made of the different ways in which it can be achieved. The processes which dictate crystal-size enlargement, either during free particle flow or during fixed particle entrapment, are described. Modulators of these processes are classified in terms of their mode of action on particular steps in the mechanism rather than on their molecular weight or size. Three different approaches for describing stone formation mechanisms are summarized. These involve mathematical models, qualitative step-by-step pathways, and qualitative non-schematic descriptions. It is suggested that although physicochemical mechanisms are crucially involved in stone formation, they do so in concert with numerous other mechanistic processes, all of which are dictated by their own specific conditions.

  7. Rapid gas hydrate formation process (United States)

    Brown, Thomas D.; Taylor, Charles E.; Unione, Alfred J.


    The disclosure provides a method and apparatus for forming gas hydrates from a two-phase mixture of water and a hydrate forming gas. The two-phase mixture is created in a mixing zone which may be wholly included within the body of a spray nozzle. The two-phase mixture is subsequently sprayed into a reaction zone, where the reaction zone is under pressure and temperature conditions suitable for formation of the gas hydrate. The reaction zone pressure is less than the mixing zone pressure so that expansion of the hydrate-forming gas in the mixture provides a degree of cooling by the Joule-Thompson effect and provides more intimate mixing between the water and the hydrate-forming gas. The result of the process is the formation of gas hydrates continuously and with a greatly reduced induction time. An apparatus for conduct of the method is further provided.

  8. Molecule Formation on Interstellar Grains (United States)

    Vidali, G.


    The first experiments that were expressively designed to be applicable to hydrogen formation reactions in the ISM measured the efficiency of formation of molecular hydrogen on a polycrystalline olivine (Pirronello et al. (1997a)). It soon turned out that more was needed, and research began on the mechanism of reaction, on the in uence of the surface morphology, and on the excitation of the just- ormed molecule. In this review, I summarize what we learned from these and other experiments, and where more work is needed: in the elementary steps of reaction, in the bridging of the laboratory-ISM gap (large ux/large surface - small ux/small grain) using simulations, and in using realistic samples of dust grains. Understanding what experiments can and cannot deliver will help in designing and targeting observations, and vice-versa.

  9. Biofilm formation on abiotic surfaces

    DEFF Research Database (Denmark)

    Tang, Lone


    Bacteria can attach to any surface in contact with water and proliferate into complex communities enclosed in an adhesive matrix, these communities are called biofilms. The matrix makes the biofilm difficult to remove by physical means, and bacteria in biofilm can survive treatment with many...... antibiotics, disinfectants and cleaning agents. Biofilms are therefore very difficult to eradicate, and an attractive approach to limit biofilm formation is to reduce bacterial adhesion. In this thesis it was shown that lowering the surface roughness had a greater effect on bacterial retention compared....... The ability to form biofilms, the amount of eDNA produced, and the importance of eDNA for biofilm formation or stability did not correlate and varied from strain to strain. Finally, a method was developed for immobilization of living bacteria for analysis by atomic force microscopy (AFM). AFM is used...

  10. Coevolutionary modeling in network formation

    KAUST Repository

    Al-Shyoukh, Ibrahim


    Network coevolution, the process of network topology evolution in feedback with dynamical processes over the network nodes, is a common feature of many engineered and natural networks. In such settings, the change in network topology occurs at a comparable time scale to nodal dynamics. Coevolutionary modeling offers the possibility to better understand how and why network structures emerge. For example, social networks can exhibit a variety of structures, ranging from almost uniform to scale-free degree distributions. While current models of network formation can reproduce these structures, coevolutionary modeling can offer a better understanding of the underlying dynamics. This paper presents an overview of recent work on coevolutionary models of network formation, with an emphasis on the following three settings: (i) dynamic flow of benefits and costs, (ii) transient link establishment costs, and (iii) latent preferential attachment.

  11. Othering, identity formation and agency

    Directory of Open Access Journals (Sweden)

    Sune Qvotrup Jensen


    Full Text Available The article examines the potentials of the concept of othering to describe identity formation among ethnic minorities. First, it outlines the history of the concept, its contemporary use, as well as some criticisms. Then it is argued that young ethnic minority men in Denmark are subject to intersectional othering, which contains elements of exoticist fascination of the other. On the basis of ethnographic material, it is analysed how young marginalized ethnic minority men react to othering. Two types of reactions are illustrated: 1 capitalization on being positioned as the other, and 2 refusing to occupy the position of the other by disidentification and claims to normality. Finally, it is argued that the concept of othering is well suited for understanding the power structures as well as the historic symbolic meanings conditioning such identity formation, but problematic in terms of agency.

  12. Spray formation with complex fluids

    Energy Technology Data Exchange (ETDEWEB)

    Lustig, S; Rosen, M, E-mail: [Grupo de Medios Porosos, Facultad de Ingenieria, Universidad de Buenos Aires, LIA (Laboratoire International Associe).Argentina (Argentina)


    Droplet formation through Faraday excitation has been tested in the low driving frequency limit. Kerosene was used to model liquid fuel with the addition of PIB in different proportions. All fluids were characterized in detail. The mechanisms of ejection were investigated to identify the relative influence of viscosity and surface tension. It was also possible to characterize the type of instability leading to the emission drop process.

  13. IRIG Serial Time Code Formats (United States)


    and G. It should be noted that this standard reflects the present state of the art in serial time code formatting and is not intended to constrain...separation for visual resolution. The LSB occurs first except for the fractional seconds subword that follows the day-of-year subword. The BCD TOY code...and P6 to complete the BCD time code word. An index marker occurs between the decimal digits in each subword to provide separation for visual

  14. The multifaceted planetesimal formation process

    DEFF Research Database (Denmark)

    Johansen, Anders; Blum, Jürgen; Tanaka, Hidekazu


    range of particle sizes, from mm to 10 m, concentrate in the turbulent gas flow. Overdense filaments fragment gravitationally into bound particle clumps, with most mass entering planetesimals of contracted radii from 100 to 500 km, depending on local disc properties. We propose a hybrid model...... for planetesimal formation where particle growth starts unaided by self-gravity but later proceeds inside gravitationally collapsing pebble clumps to form planetesimals with a wide range of sizes....

  15. DAC tries a new format

    DEFF Research Database (Denmark)

    Andersen, Jens Enevold Thaulov; Karlberg, Bo; Emons, Hendrik


    The European Analytical Column has a somewhat different format this time. From now and on it is our ambition to invite a guest columnist to give her/his view on various matters related to Analytical Chemistry in Europe. This year we have invited Prof. Hendrik Emons at the Institute for Reference...... regarding guidelines for the content of the education in analytical chemistry at the BSc level (Eurobachelor) [1]....

  16. Neutron Stars: Formation and Structure


    Kutschera, Marek


    A short introduction is given to astrophysics of neutron stars and to physics of dense matter in neutron stars. Observed properties of astrophysical objects containing neutron stars are discussed. Current scenarios regarding formation and evolution of neutron stars in those objects are presented. Physical principles governing the internal structure of neutron stars are considered with special emphasis on the possible spin ordering in the neutron star matter.


    The report gives results of experiments to assess: (1) the effect of residual copper retained in an incineration facility on polychlorinated dibenzo-p-dioxin and dibenzofuran (PCDD/PCDF) formation during incineration of non-copper-containing chlorofluorocarbons (CFCs); and (2) the formation of chlorinated and aromatic products of incomplete combustion (PICs), including PCDD/PCDFs, during incineration of CFC recycling residue and hydrochlorofluorocarbons (HCFCs). High concentrations of PCDD/PCDFs (23,800 ng/dscm at 7% O2) measured in FY91 during incineration of CFC-12 in a turbulent flame reactor (TFR) could not be repeated in the present study. Repetition tests conducted in the same facility under similar operating conditions resulted in PCDD/PCDF concentrations of 118ng/dscm at 7% O2. However, results of the present study suggest that residual copper retained in an incineration facility possibly promotes the formation of PCDD/PCDFs during incineration of CFC-12 which does not contain copper. Tests conducted in the TFR resulted in measured PCDD/PCDF concentrations of 386-454 ng/dscm at 7% O2 during incineration of CFC-12 which followed incineration of copper-containing compounds. These results suggest that CFCs may best be incinerated in incinerators which do not treat any copper-containing waste prior to CFC incineration. Report available at NTIS as PB96152186. To share information

  18. Interactions, Starbursts, and Star Formation

    Directory of Open Access Journals (Sweden)

    Johan H. Knapen


    Full Text Available We study how interactions between galaxies affect star formation within them by considering a sample of almost 1500 of the nearest galaxies, all within a distance of ∼45 Mpc. We use the far-IR emission to define the massive star formation rate (SFR, and then normalise the SFR by the stellar mass of the galaxy to obtain the specific star formation rate (SSFR. We explore the distribution of (SSFR with morphological type and with stellar mass. We calculate the relative enhancement of SFR and SSFR for each galaxy by normalising them by the median SFR and SSFR values of individual control samples of similar non-interacting galaxies. We find that both the median SFR and SSFR are enhanced in interacting galaxies, and more so as the degree of interaction is higher. The increase is moderate, reaching a maximum of a factor of 1.9 for the highest degree of interaction (mergers. While the SFR and SSFR are enhanced statistically by interactions, in many individual interacting galaxies they are not enhanced at all. Our study is based on a representative sample of nearby galaxies and should be used to place constraints on studies based on samples of galaxies at larger distances.

  19. Formation of Outer Planets: Overview (United States)

    Lissauer, Jack


    An overview of current theories of planetary formation, with emphasis on giant planets is presented. The most detailed models are based upon observation of our own Solar System and of young stars and their environments. Terrestrial planets are believe to grow via pairwise accretion until the spacing of planetary orbits becomes large enough that the configuration is stable for the age of the system. According to the prevailing core instability model, giant planets begin their growth by the accumulation of small solid bodies, as do terrestrial planets. However, unlike terrestrial planets, the growing giant cores become massive enough that they are able to accumulate substantial amounts of gas before the protoplanetary disk disspates. The primary questions regarding the core instability model is whether planets with small cores can accrete gaseous enveloples within the lifetimes of gaseous protoplanetary disks. The main alternative giant planet formation model is the disk instability model, in which gaseous planets form directly via gravitational instabilities within protoplanetary disks. Formation of giant planets via gas instability has never been demonstrated for realistic disk conditions. Moreover, this model has difficulty explaining the supersolar abundances of heavy elements in Jupiter and Saturn, and it does not explain the orgin of planets like Uranus and Neptune.

  20. Star Formation in Tadpole Galaxies

    Directory of Open Access Journals (Sweden)

    Casiana Muñoz-Tuñon


    Full Text Available Tadpole Galaxies look like a star forming head with a tail structure to the side. They are also named cometaries. In a series of recent works we have discovered a number of issues that lead us to consider them extremely interesting targets. First, from images, they are disks with a lopsided starburst. This result is rmly  established with long slit spectroscopy in a nearby representative sample. They rotate with the head following the rotation pattern but displaced from the rotation center. Moreover, in a search for extremely metal poor (XMP galaxies, we identied tadpoles as the dominant shapes in the sample - nearly 80% of the local XMP galaxies have a tadpole morphology. In addition, the spatially resolved analysis of the metallicity shows the remarkable result that there is a metallicity drop right at the position of the head. This is contrary to what intuition would say and dicult to explain if star formation has happened from gas processed in the disk. The result could however be understood if the star formation is driven by pristine gas falling into the galaxy disk. If conrmed, we could be unveiling, for the rst time, cool  ows in action in our nearby world. The tadpole class is relatively frequent at high redshift - 10% of resolvable galaxies in the Hubble UDF but less than 1% in the local Universe. They are systems that could track cool ows and test models of galaxy formation.

  1. Hypothesis Formation, Paradigms, and Openness

    Directory of Open Access Journals (Sweden)

    Conrad P. Pritscher


    Full Text Available A part of hypothesis formation, while necessary for scientific investigation, is beyond direct observation. Powerful hypothesis formation is more than logical and is facilitated by mind­opening. As Percy Bridgeman, Nobel laureate, said, science is: “Nothing more than doing one's damnedest with one's mind, no holds barred.” This paper suggests more open schooling helps generate more open hypothesizing which helps one do one's damnedest with one's mind. It is hypothesized that a more open process of hypothesis formation may help schools and society forge new ways of living and learning so that more people more often can do their damnedest with their mind. This writing does not offer a new paradigm but rather attempts to elaborate on the notion that new paradigms are difficult to form without openness to what was previously quasi­unthinkable. More on these topics and issues is included in the author's Reopening Einstein's Thought: About What Can't Be Learned From Textbooks ­­to be published by Sense Publishers in June 2008.

  2. Theory of Planetary System Formation (United States)

    Cassen, Patrick


    Observations and theoretical considerations support the idea that the Solar System formed by the collapse of tenuous interstellar matter to a disk of gas and dust (the primitive solar nebula), from which the Sun and other components separated under the action of dissipative forces and by the coagulation of solid material. Thus, planets are understood to be contemporaneous byproducts of star formation. Because the circumstellar disks of new stars are easier to observe than mature planetary systems, the possibility arises that the nature and variety of planets might be studied from observations of the conditions of their birth. A useful theory of planetary system formation would therefore relate the properties of circumstellar disks both to the initial conditions of star formation and to the consequent properties of planets to those of the disk. Although the broad outlines of such a theory are in place, many aspects are either untested, controversial, or otherwise unresolved; even the degree to which such a comprehensive theory is possible remains unknown.

  3. Mechanism of formation of hydantoins

    Energy Technology Data Exchange (ETDEWEB)

    Worman, J.J.; Uhrich, K.; Olson, E.; Diehl, J.; Farnum, S.; Hawthorne, S.


    Hydantoins, (I), are biologically active compounds which occur in nature and have been isolated from such sources as sugar beets and butterfly wings. Synthetic analogs have found widespread use as anticonvulsant drugs, bacteriocides, stabilizers in photographic film, and in the preparation of high temperature epoxy resins. Uses, preparation, and reactions of hydantoins have been extensively studied and are reported elsewhere. Recently a number of hydantoin isomers have been detected and identified in coal gasification condensate water from the gasification of Indian Head (ND) lignite in a slagging fixed bed gasifier. The hydantoins constitute a major portion of the organics in the condensate water. In generation the concentrations of hydantoins found in the condensate water from an ash gasifier are smaller than those found from the slagging process. Lignite coal reacting in a slagging gasifier gives the largest concentration of hydantoins in the condensate water. The major isomer in the coal gasification condensate water is 5,5-dimethylhydantoin (DMH). DMH is formed from individual species (acetone, cyanide, ammonia, and carbonate) in the condensate water and does not form directly in the gasifier. The rate of formation of DMH was first order in all of the reactants as expressed by the equation: Rate of formation of DMH = (Acetone) (HCN) (NH/sub 3/) (CO/sub 2/). This kinetic data is valuable in predicting the rate of formation of DMH in coal gasification condensate water, provided the model is applicable. A mechanism consistent with the kinetic data and partially verified by others is shown in Scheme 1. 13 refs.

  4. Strategy Formation in Eastern Jutland

    DEFF Research Database (Denmark)

    Olesen, Kristian


      In Eastern Jutland a strategy formation process has been initiated with the aim of developing a strategic spatial plan for the city region.  An organisation has been set up to deal with the first phase of the process, which is to carry out three functional analyses and prepare a common vision...... for Eastern Jutland.  This process relies on the municipalities in Eastern Jutland reaching consensus on a common vision for the city region.   The key issue in spatial planning in Eastern Jutland is a conflict between preserving the high quality of landscape and maintaining the divide between the countryside...


    Rudbach, Jon A.; Milner, Kelsey C.; Ribi, Edgar


    When endotoxins extracted from enteric bacteria were mixed in the presence of sodium deoxycholate, and the bile salt was subsequently removed by dialysis or by extraction with ethanol, a new type of endotoxin was formed. The latter material was as biologically active as the original endotoxins and possessed a combination of antigenic determinants that were previously unique to each of the individual endotoxins in the mixture. This hybrid formation between endotoxins was detected by immunodiffusion and radioautography and by quantitative precipitation procedures. PMID:4961269

  6. Nations: Formation and Interest Dynamics

    Directory of Open Access Journals (Sweden)

    A E Kafirin


    Full Text Available The article gives a brief description of historic conditions leading to the formation of nations, and on the basis of attributive features of a nation the set of national interests is identified. Considering national interests as a variable, the author stresses that they depend both on the wishes of ordinary people and on the ability of the state to streamline them in a constructive manner. National interests are classified according to their dynamic characteristics, and the special role of the state as the mouthpiece of national interests is pointed out.

  7. Adsorption-induced step formation

    DEFF Research Database (Denmark)

    Thostrup, P.; Christoffersen, Ebbe; Lorensen, Henrik Qvist


    Through an interplay between density functional calculations, Monte Carlo simulations and scanning tunneling microscopy experiments, we show that an intermediate coverage of CO on the Pt(110) surface gives rise to a new rough equilibrium structure with more than 50% step atoms. CO is shown to bin...... so strongly to low-coordinated Pt atoms that it can break Pt-Pt bonds and spontaneously form steps on the surface. It is argued that adsorption-induced step formation may be a general effect, in particular at high gas pressures and temperatures....

  8. Quality guidance and quality formation

    DEFF Research Database (Denmark)

    Poulsen, Carsten Stig; Juhl, Hans Jørn; Kristensen, Kai


    This paper presents an extension of the quality guidance model of Steenkamp and van Trijp that includes consumer quality formation processes. Quality expectations and quality experiences are seen as antecedents of perceived overall product quality conceptual model is applied using LISREL to a data...... set on Danish butter cookies. Five plausible models of the relation between expectation, experience and perceived product quality are estimated. Finally one model is selected on the basis of three criteria: chi-square, RMSEA and AIC: The results show a model where expectations are indirectly related...... to perceived quality through experience. Udgivelsesdato: APR...

  9. Stability on Adaptive NN Formation Control with Variant Formation Patterns and Interaction Topologies

    Directory of Open Access Journals (Sweden)

    Xin Chen


    Full Text Available The formation task achieved by multiple robots is a tough issue in practice, because of the limitations of the sensing abilities and communicating functions among them. This paper investigates the decentralized formation control in case of parameter uncertainties, bounded disturbances, and variant interactions among robots. To design decentralized controller, a formation description is firstly proposed, which consists of two aspects in terms of formation pattern and interaction topology. Then the formation control using adaptive neural network (ANN is proposed based on the relative error derived from formation description. From the analysis on stability of the formation control under invariant/variant formation pattern and interaction topology, it is concluded that if formation pattern is of class Ck, k ≥1, and interaction graph is connected and changed with finite times, the convergence of the formation control is guaranteed, so that robots must form the formation described by the formation pattern.

  10. Tube Formation in Nanoscale Materials

    Directory of Open Access Journals (Sweden)

    Yan Chenglin


    Full Text Available Abstract The formation of tubular nanostructures normally requires layered, anisotropic, or pseudo-layered crystal structures, while inorganic compounds typically do not possess such structures, inorganic nanotubes thus have been a hot topic in the past decade. In this article, we review recent research activities on nanotubes fabrication and focus on three novel synthetic strategies for generating nanotubes from inorganic materials that do not have a layered structure. Specifically, thermal oxidation method based on gas–solid reaction to porous CuO nanotubes has been successfully established, semiconductor ZnS and Nb2O5nanotubes have been prepared by employing sacrificial template strategy based on liquid–solid reaction, and an in situ template method has been developed for the preparation of ZnO taper tubes through a chemical etching reaction. We have described the nanotube formation processes and illustrated the detailed key factors during their growth. The proposed mechanisms are presented for nanotube fabrication and the important pioneering studies are discussed on the rational design and fabrication of functional materials with tubular structures. It is the intention of this contribution to provide a brief account of these research activities.

  11. Pattern Formation and Infinite Sets (United States)

    Berezin, Alexander A.


    In polynomial simulations of pattern formation and self-organization (e.g., bifurcational cascades, fractal structures of Julia sets, cosmogenesis, bioevolution), starting from rational points and coefficients leaves all subsequent iterations rational. While rational points have zero Lebesgue measure, they are dense everywhere in any N-dimensional space and hence any iteration trail or fractal structure at any level of resolution can be constructed with arbitrary precision using subset of only rational points. Any geometrical object or pattern can be spanned over rational points (pixels) with arbitrary precision. Since all rationals can be one-to-one counted by set of all integers, or any infinite subset of them (e.g., by primes), any pattern spanned over rationals can be translated into pattern spanned over, say, only (counting) primes. Since counting rationals by any set of integers always forms jumpwise mapping (nonmonotonic bijectivity), resulting translation of connected pattern (say, 3D Euclidean, or 4D Einsteinian) appears chaotic, but can always be finitely descrambled (Arnold's Cat analogy). Simplest model when all rational points on x-axis are Cantor-counted by set of all primes results in infinitude of never-repeating primes on any arbitrary small epsilon-interval (fractal hologram with infinite depth). This potential "Platonic Library written in primes" provides infinite "reference" resource for pattern formation dynamics.

  12. Coring in deep hardrock formations

    Energy Technology Data Exchange (ETDEWEB)

    Drumheller, D.S.


    The United States Department of Energy is involved in a variety of scientific and engineering feasibility studies requiring extensive drilling in hard crystalline rock. In many cases well depths extend from 6000 to 20,000 feet in high-temperature, granitic formations. Examples of such projects are the Hot Dry Rock well system at Fenton Hill, New Mexico and the planned exploratory magma well near Mammoth Lakes, California. In addition to these programs, there is also continuing interest in supporting programs to reduce drilling costs associated with the production of geothermal energy from underground sources such as the Geysers area near San Francisco, California. The overall progression in these efforts is to drill deeper holes in higher temperature, harder formations. In conjunction with this trend is a desire to improve the capability to recover geological information. Spot coring and continuous coring are important elements in this effort. It is the purpose of this report to examine the current methods used to obtain core from deep wells and to suggest projects which will improve existing capabilities. 28 refs., 8 figs., 2 tabs.

  13. Cataract formation after penetrating keratoplasty. (United States)

    Rathi, V M; Krishnamachary, M; Gupta, S


    To assess the incidence and risk factors for developing cataract after penetrating keratoplasty (PKP). L.V. Prasad Eye Institute, Hyderabad, India. This retrospective analysis of 251 phakic patients who had PKP between 1987 and 1994 assessed the incidence of and risk factors for cataract formation. Patients were assigned to one of two groups: Group 1 (n = 96), patients with keratoconus and corneal dystrophy; Group 2 (n = 88), patients with corneal scar and adherent leucoma. Preoperative, intraoperative, and postoperative lens details were noted. Data on intraoperative iris procedures (excess manipulation, pupilloplasty, synechiolysis, peripheral iridectomy) and postoperative topical steroid usage were assessed. Sixty-seven patients were excluded because of incomplete lens status data. Of the remaining 184 patients, 45 (24.45%) developed cataract. Most cataracts (n = 31) developed within the first year of surgery. The incidence of cataract was significantly higher in Group 2 (n = 29) than in Group 1 (n = 16) (P = .0102). There was no significant between-group difference in mean steroid dose (P = .7064); however, the mean dose was significantly higher in eyes with cataracts (563 +/- 234 units) than in those without (479 +/- 127 units) (P = .0352). In Group 2, 9 of 20 patients who had synechiolysis, 1 of 3 who had pupilloplasty, and 2 of 5 who had peripheral iridectomy developed cataract. In Group 1, no patient had iris-related procedures. Excessive steroid use and intraoperative iris manipulations are major risk factors for cataract formation after PKP.

  14. Deep Landslides in flysch formations (United States)

    Marinos, Vassilis


    Flysch, linked with the tectonic development of an area, has suffered from compressional forces being highly deformed by thrust faults and folds, containing thus often tectonically pre-sheared zones of various size. These geological characteristics may produce weak to very weak rock masses which may present instability and landslides in both mountain and local slope scale. The paper mainly discusses the "mountain" scale phenomena. The size of these masses can reach hundreds of meters in both depth and width on the valley sides. A brief presentation of the flysch formation is presented. A typology is presented with 11 types of flysch, depending on the persistence and participation or not of the strong members (as sandstones) against the weak ones (as siltstones, shales) and the degree and scale of tectonic disturbance. These rock mass types are connected with the landslide mechanism. In all cases the tectonic conditions of a broader area are responsible and the establishment of the tectonic-paleogeographic model is necessary before the conceptual study and design of any major infrastructure work and the choice of its alignment or location. Given the size of such instability areas remedial measures are in most cases not feasible and the realignment or relocation from the initial plans are often the only solution. Cases from highways and pipelines in Greek and Albanian territory are presented. A large number of information from lab tests, geotechnical classifications and back analyses collected from a wide variety of flysch formations is presented and discussed.

  15. Beaver assisted river valley formation (United States)

    Westbrook, C.J.; Cooper, D.J.; Baker, B.W.


    We examined how beaver dams affect key ecosystem processes, including pattern and process of sediment deposition, the composition and spatial pattern of vegetation, and nutrient loading and processing. We provide new evidence for the formation of heterogeneous beaver meadows on riverine system floodplains and terraces where dynamic flows are capable of breaching in-channel beaver dams. Our data show a 1.7-m high beaver dam triggered overbank flooding that drowned vegetation in areas deeply flooded, deposited nutrient-rich sediment in a spatially heterogeneous pattern on the floodplain and terrace, and scoured soils in other areas. The site quickly de-watered following the dam breach by high stream flows, protecting the deposited sediment from future re-mobilization by overbank floods. Bare sediment either exposed by scouring or deposited by the beaver flood was quickly colonized by a spatially heterogeneous plant community, forming a beaver meadow. Many willow and some aspen seedlings established in the more heavily disturbed areas, suggesting the site may succeed to a willow carr plant community suitable for future beaver re-occupation. We expand existing theory beyond the beaver pond to include terraces within valleys. This more fully explains how beavers can help drive the formation of alluvial valleys and their complex vegetation patterns as was first postulated by Ruedemann and Schoonmaker in 1938. ?? 2010 John Wiley & Sons, Ltd.

  16. Factors Influencing New Business Formation

    Directory of Open Access Journals (Sweden)

    Andreea-Oana Iacobuţă


    Full Text Available The purpose of this study is to identify the factors that influence the creation of new businesses and to point out both the differences and the similarities existing between countries and groups of countries in terms of these influencing factors. We are mainly interested in the place Romania and Bulgaria have among the countries of the world from the perspective of entrepreneurial spirit and its influencing factors. To capture the level of business formation we use New business density from World Bank Doing business. Drawing on the existing literature we consider for our analysis several indicators related to economic environment such as GDP per capita, unemployment rate, inflation rate, the level of taxes, foreign direct investments and public debt and indicators describing the quality of governance. The research uses 2014 data for 67 countries, from all development categories, collected from Heritage Foundation database. The research results obtained with principal components analysis show that good governance results in higher levels of GDP per capita and income taxes and the increase of the level of business formation. Also, good governance leads to a decrease in inflation and unemployment. Furthermore, the hierarchical cluster analysis is used to identify groups of countries and to outline similarities and differences between them.


    Directory of Open Access Journals (Sweden)

    Nediljka Gaurina-Međimurec


    Full Text Available Formate-based fluids has been successfully used in over hunders HPHT well operations since they introduced in field practice. They have many advantages when compared with conventional HPHT drilling and completion fluids such as: minimal formation damage, maintenance of additve properties at high temperatures, reduced hydraulic flow resistance, low potential for differential sticking, naturally lubricating, very low corrosion rates, biodegradable and pose little risk to the environment etc. Formate-based fluids can be applied during deep slim hole drilling, shale drilling, reservoir drilling, salt and gas hydrate formations drilling. The laboratory research was carried out to evaluate the rheological behavior of formate-based fluids as a function of temperature. Formate-based fluids were formulated using potassium formate brine, xanthan polymer, PAC, starch and calcium carbonate. Experimental results show that potassium formate improves the thermal stability of polymers.

  18. Satellite Formation Control Using Atmospheric Drag

    National Research Council Canada - National Science Library

    Hajovsky, Blake B


    This study investigates the use of a linear quadratic terminal controller to reconfigure satellite formations using atmospheric drag actuated control while minimizing the loss of energy of the formation...

  19. Advanced modulation formats for transmission systems

    DEFF Research Database (Denmark)

    Tokle, Torger; Serbay, M.; Jensen, Jesper Bevensee


    DQPSK has shown that multilevel modulation formats can enable new possibilities in optical communication systems. The paper presents several options to go to even more advanced formats using direct detection....

  20. Towards Verification of Rydberg Positronium Formation

    CERN Document Server

    Fenton, Eliot


    In this report I describe progress towards the formation of Rydberg positronium. Though more runs are required to gain statistical signifi- cance, there are promising signs that could signal Rydberg positronium formation.

  1. Intrahepatic Biliary Cystadenoma With Intracystic Gallstone Formation


    Shize Lei; Domenico, Don R.; Howard, John M.


    Biliary cystadenoma is a rare tumor of the liver. We describe a biliary cystadenoma of the left lobe of the liver with intracystic gallstone formation. This is the first report of stone formation in biliary cystadenoma of the liver.

  2. Choosing to write the paper format thesis. (United States)

    Morris, H M; Tipples, G


    Graduate students today may be faced with the option of writing either a traditional format thesis or a paper format thesis. In contrast to the traditional format in which the text body consists of four or five chapters, the body of the paper format thesis can be comprised of an introductory chapter, two or more papers written as publishable manuscripts, and a conclusion. In this article, an overview of the paper format thesis is presented and contrasted with the traditional format thesis. The description of the paper format thesis is followed by its advantages and disadvantages for writers and readers. It is by weighing all possible pros and cons, as well as considering one's individual situation, that the graduate student will be able to decide which format of thesis to write.

  3. Package Formats for Preserved Digital Material

    DEFF Research Database (Denmark)

    Zierau, Eld


    This paper presents an investigation of the best suitable package formats for long term digital preservation. The choice of a package format for preservation is crucial for future access, thus a thorough analysis of choice is important. The investigation presented here covers setting up requireme......This paper presents an investigation of the best suitable package formats for long term digital preservation. The choice of a package format for preservation is crucial for future access, thus a thorough analysis of choice is important. The investigation presented here covers setting up...... requirements for package formats used for long term preserved digital material, and using these requirements as the basis for analysing a range of package formats. The result of the concrete investigation is that the WARC format is the package format best suited for the listed requirements. Fulfilling......, e.g. if there are specific forensic or direct access to files....

  4. Formation dancing[New generation formation evaluation tools

    Energy Technology Data Exchange (ETDEWEB)

    Flatern, Rick von


    The justification for the faith placed in the new generation of formation evaluation tools by the three major players (Baker Hughes, Halliburton and Schlumberger) in the provision of oilfield services is investigated. The oil and gas industry is now working in water depths thought unworkable just a few years ago but it is essential that time on site is used to maximum effect. The three companies have all developed new technologies to short-circuit the time spent on well tests. The article is presented under the sub-headings of (i) contamination (refers to contamination of reservoir samples with drilling fluid); (ii) drawdown and sandface control; (iii) transportation and (iv) replacing the flow test.

  5. Treating nahcolite containing formations and saline zones

    Energy Technology Data Exchange (ETDEWEB)

    Vinegar, Harold J


    A method for treating a nahcolite containing subsurface formation includes removing water from a saline zone in or near the formation. The removed water is heated using a steam and electricity cogeneration facility. The heated water is provided to the nahcolite containing formation. A fluid is produced from the nahcolite containing formation. The fluid includes at least some dissolved nahcolite. At least some of the fluid is provided to the saline zone.

  6. The void galaxy survey: Star formation properties

    NARCIS (Netherlands)

    Beygu, B.; Kreckel, K.; van der Hulst, J. M.; Jarrett, T. H.; Peletier, R.; van de Weygaert, R.; van Gorkom, J. H.; Aragon-Calvo, M. A.


    We study the star formation properties of 59 void galaxies as part of the Void Galaxy Survey (VGS). Current star formation rates are derived from H α and recent star formation rates from near-UV imaging. In addition, infrared 3.4, 4.6, 12 and 22 μm Wide-field Infrared Survey Explorer emission is

  7. Theoretical Considerations of Massive Star Formation (United States)

    Yorke, Harold W.


    This viewgraph presentation reviews the formation of massive stars. The formation of massive stars is different in many ways from the formation of other stars. The presentation shows the math, and the mechanisms that must be possible for a massive star to form.

  8. Resource description framework triples entity formations using ...

    African Journals Online (AJOL)

    A method in formatting unstructured sentences from the source corpus to a specific knowledge representation such as RDF is needed. A method for RDF entity formations from a paragraph of text using statistical language model based on N-gram is introduced. The implementation of RDF entity formation is applied on ...

  9. String Formatting Considered Harmful for Novice Programmers (United States)

    Hughes, Michael C.; Jadud, Matthew C.; Rodrigo, Ma. Mercedes T.


    In Java, "System.out.printf" and "String.format" consume a specialised kind of string commonly known as a format string. In our study of first-year students at the Ateneo de Manila University, we discovered that format strings present a substantial challenge for novice programmers. Focusing on their first laboratory we found…

  10. New Office Software course format

    CERN Multimedia

    HR Department


    Always interested to anticipate your training needs, the Technical Training service is pleased to propose two new Office Software course formats : “Focus on... ” : On a monthly basis we will propose a theme such as “Sharepoint Collaboration Workspace” or “Word 2007” or “charts” etc. You will have to send us in advance your questions regarding the proposed topic and register for the course through our Training Catalogue. During the session, our trainer will answer all the questions received and participants will increase their knowledge thanks to the solutions discussed for everyone. The course will last two hours, from 09h00 to 11h00 - with open questions on the proposed topic at the end. “Office software Individual coaching”: If one or several particular topics cause you sleepless nights, you can get the help of our trainer who will come to your workplace for a multiple of 1-hour slots . All fields ...

  11. Mud Volcanoes Formation And Occurrence (United States)

    Guliyev, I. S.


    Mud volcanoes are natural phenomena, which occur throughout the globe. They are found at a greater or lesser scale in Azerbaijan, Turkmenistan, Georgia, on the Kerch and Taman peninsulas, on Sakhalin Island, in West Kuban, Italy, Romania, Iran, Pakistan, India, Burma, China, Japan, Indonesia, Malaysia, New Zealand, Mexico, Colombia, Trinidad and Tobago, Venezuela and Ecuador. Mud volcanoes are most well-developed in Eastern Azerbaijan, where more than 30% of all the volcanoes in the world are concentrated. More than 300 mud volcanoes have already been recognized here onshore or offshore, 220 of which lie within an area of 16,000 km2. Many of these mud volcanoes are particularly large (up to 400 m high). The volcanoes of the South Caspian form permanent or temporary islands, and numerous submarine banks. Many hypotheses have been developed regarding the origin of mud volcanoes. Some of those hypotheses will be examined in the present paper. Model of spontaneous excitation-decompaction (proposed by Ivanov and Guliev, 1988, 2002). It is supposed that one of major factors of the movement of sedimentary masses and formation of hydrocarbon deposits are phase transitions in sedimentary basin. At phase transitions there are abnormal changes of physical and chemical parameters of rocks. Abnormal (high and negative) pressure takes place. This process is called as excitation of the underground environment with periodicity from several tens to several hundreds, or thousand years. The relationship between mud volcanism and the generation of hydrocarbons, particularly methane, is considered to be a critical factor in mud volcano formation. At high flow rates the gas and sediment develops into a pseudo-liquid state and as flow increases the mass reaches the "so-called hover velocity" where mass transport begins. The mass of fluid moves as a quasi-uniform viscous mass through the sediment pile in a piston like manner until expelled from the surface as a "catastrophic eruption

  12. Accretion Processes in Star Formation

    DEFF Research Database (Denmark)

    Küffmeier, Michael

    that the accretion process of stars is heterogeneous in space, time and among different protostars. In some cases, disks form a few thousand years after stellar birth, whereas in other cases disk formation is suppressed due to efficient removal of angular momentum. Angular momentum is mainly transported outward......Stars and their corresponding protoplanetary disks form in different environments of Giant Molecular Clouds. By carrying state-of-the art zoom-simulations with the magnetohydrodynamical code ramses, I investigated the accretion process around young stars that are embedded in such different...... for short-lived radionuclides that enrich the cloud as a result of supernova explosions of the massive stars allows us to analyze the distribution of the short-lived radionuclides around young forming stars. In contradiction to results from highly-idealized models, we find that the discrepancy in 26 Al...

  13. Magnetogravitodynamics model of galaxy formation (United States)

    Greyber, Howard D.


    Unless the tau neutrino is discovered to have an appropriate size mass, the theories of galaxy formation involving only gravity may not fit the observations. A model within the Big Bang hypothesis is described with processes occurring after ``Breakout'' that lead to the concentration of matter along thin spatially curved current sheets. When a critical density is reached, gravitational collapse occurs, forming galaxies in many places along thin spatially curved sheets surrounding huge voids, in a cellular structure, as in the observations by Geller, Huchra et al. The origin of a primordial magnetic field concentrated in the sheets is explained. The model predicts that much of the missing dark matter is located along the thin spatially curved sheets of galaxies.

  14. Restucturing the Project Work Format

    DEFF Research Database (Denmark)

    Dupont, Søren


    The chapter is based on an evaluation of a pedagogical experiment at Roskilde University, the Antology Experiment. The objective of the experiment was to develop and expand the framework for project work through the production of anthologies compiled collectively by a number of project groups....... The novel aspects of the Anthology Experiment were most notably its magnitude and complexity. In this experiment the groups were totalling some 50 students who were working together. The experiment used a well-known publishing format from research, namely the anthology form, which usually focuses...... on a specific research topic and includes contributions from various researchers. In the Anthology Experiment, the project groups could be viewed as ‘research units’ that produce the contributions to the anthology. The complexity of the experiment offered challenges, both for students and supervisors...

  15. Cryosalt Formation in Delaminated Clays (United States)

    Yeşilbaş, Merve; Boily, Jean-François


    Hydrohalite (NaClṡ2H2O) forms by evaporation, sublimation and freezing of aqueous solutions of NaCl. Although this process is traditionally deemed to occur in aqueous solutions little attention has been paid on whether this is possible with minerals. Smectite minerals are particularly interesting in this regard for their ability to accommodate water between aluminosilicate sheets, allowing them to swell and even delaminate. In particular, montmorillonite possesses high affinities for water and can play important roles in water retention and ice formation in nature, as well as in strategies for nuclear waste storage and even for technological applications. [1,2] For this study, we aimed to develop insight into the molecular-level nature of hydrohalite formation at surfaces of montmorillonite particles as well as in their interlayers. Thin films of Na+ and Ca2+ exchanged montmorillonites deposited on a diamond-based Attenuated Total Reflectance (ATR) cell were interacted with (0.01, 0.1, 1 and 5M) NaCl solutions, and then frozen to -10˚ C. The resulting frozen montmorillonites pastes were then probed by ATR Fourier Transform Infrared (FTIR) spectroscopy, which is a highly sensitive technique for probing hydrogen bonding in minerals and water. Our results on Na-montmorillonite showed that hydrohalite, with its characteristic O-H stretching (νOH ˜ 3245-3265, 3408, 3462, 3555 cm-1) and bending (δOH ˜ 1614 and 1641 cm-1) bands, formed from solutions of at least 0.1 M NaCl, yet well below the typical homogeneous crystallization of this phase from pure aqueous solutions.[3] Further analysis of the O-H stretching and silicate (νSi-O ˜1000 cm-1) regions of frozen paste of montmorillonites revealed that hydrohalite formed within interlayers and at surfaces of Na-montmorillonite. Ca-montmorillonite did not, on the other hand, promote hydrohalite formation but did undergo Ca2+/Na+ ion exchange due to exposure of the NaCl solutions. Given the inability of Ca

  16. Formation of zirconium metallic glass. (United States)

    Zhang, Jianzhong; Zhao, Yusheng


    Bulk metallic glasses are commonly produced by the rapid cooling of liquid alloys. They have emerged over the past decade as a novel class of materials, with attractive properties and technological promise. The bulk metallic glasses so far produced contain three or more component elements. These complex compositions are necessary to frustrate the crystallization of the liquid melt on cooling, but can also lead to phase separation, which is detrimental to the thermal and mechanical properties of metallic glasses. Here we report, using X-ray diffraction measurements, the formation of a bulk metallic glass from elemental zirconium at high static pressures and low temperatures (relative to its melting temperature at atmospheric pressure). Amorphous zirconium can be recovered at ambient conditions and demonstrates a superior thermal stability compared to amorphous alloys, which could lead to new high-temperature applications of amorphous metals.

  17. Biofilm formation by Acinetobacter baumannii. (United States)

    Vidal, R; Dominguez, M; Urrutia, H; Bello, H; Gonzalez, G; Garcia, A; Zemelman, R


    Acinetobacter baumannii, an important nosocomial pathogen is usually found on various surfaces in the hospital environment. In this work, the ability to form biofilms on the surface of sterile coverslips by one clinical isolate of A. baumannii was studied. Sessile cells which adhered to coverslips after being immersed in a nutrient-deficient mineral medium were observed by epifluorescence and scanning electron microscopy at various times of incubation. A rapid increase in the number of sessile cells in young biofilms, followed by a slower increase of such cells was found. At 48 h biofilms were clearly visible and an amorphous material similar to the exopolysaccharide described in some other bacteria covered sessile cells was evident. Biofilm formation by A. baumannii probably favours its maintenance on solid surfaces in the hospital environment and protects the micro-organism against some antibacterial factors.

  18. Structure of structure formation theories (United States)

    Hu, Wayne; Eisenstein, Daniel J.


    We study the general structure of models for structure formation, with applications to the reverse engineering of the model from observations. Through a careful accounting of the degrees of freedom in covariant gravitational instability theory, we show that the evolution of structure is completely specified by the stress history of the dark sector. The study of smooth, entropic, sonic, scalar anisotropic, vector anisotropic, and tensor anisotropic stresses reveals the origin, robustness, and uniqueness of specific model phenomenology. We construct useful and illustrative analytic solutions that cover cases with multiple species of differing equations of state relevant to the current generation of models, especially those with effectively smooth components. We present a simple case study of models with phenomenologies similar to that of a ΛCDM model to highlight reverse-engineering issues. A critical-density universe dominated by a single type of dark matter with the appropriate stress history can mimic a ΛCDM model exactly.

  19. Selective formation of tungsten nanowires

    Directory of Open Access Journals (Sweden)

    Bien Daniel


    Full Text Available Abstract We report on a process for fabricating self-aligned tungsten (W nanowires with polycrystalline silicon core. Tungsten nanowires as thin as 10 nm were formed by utilizing polysilicon sidewall transfer technology followed by selective deposition of tungsten by chemical vapor deposition (CVD using WF6 as the precursor. With selective CVD, the process is self-limiting whereby the tungsten formation is confined to the polysilicon regions; hence, the nanowires are formed without the need for lithography or for additional processing. The fabricated tungsten nanowires were observed to be perfectly aligned, showing 100% selectivity to polysilicon and can be made to be electrically isolated from one another. The electrical conductivity of the nanowires was characterized to determine the effect of its physical dimensions. The conductivity for the tungsten nanowires were found to be 40% higher when compared to doped polysilicon nanowires of similar dimensions.

  20. Crystal formation in furunculosis agar (United States)

    Bullock, G.L.; Ross, A.J.


    SINCE ITS INTRODUCTION SOME MONTHS AGO, FURUNCULOSIS AGAR has been employed in the diagnosis of suspect furunculosis and also as a general purpose medium. During our work with this medium we have noticed discrete "colonies," of crystalline material, which very closely resemble microbial colonies. These crystal colonies are compact and appear on both the surface and subsurface; they occur in inoculated slants and plates incubated for long periods (2 to 3 weeks), as well as in uninoculated stored medium. As the crystal colonies could be confusing to workers using this medium, we decided to attempt to identify them and also to determine whether storage conditions and different lots of medium affect crystal formation.

  1. Antarctic Megadunes: Characteristics and Formation (United States)

    Scambos, T.; Fahnestock, M.; Shuman, C.; Bauer, R.


    We review field geophysical, meteorological, and remote sensing data covering Antarctica's 'megadune' regions with the purpose of constraining formation models for the features. Megadunes are striped accumulation variations, oriented perpendicular to mean katabatic windflow, with hieghts ranging from 2 to 8 meters, and crest spacing from 2 to 6 km. Crest ridges have lateral extents of up to 100 km. Upwind faces are steeper than downwind faces, and are characterized by very large, eroded sastrugi. Surface 'glazes' of ice, with coarse recrystalized grains in the subsurface, are present in the lee faces. Dunes are widespread across the East Antarctic plateau, although laterally extensive dune fields occur in just a few regions. Strong variations in surface roughness and snow grain size between crest/upwind faces and trough/downwind faces are evident in albedo and radar or visible-light backscatter. Field measurements at a site 400 km southeast of Vostok station (80.78 deg S, 124.5 deg E) provide insight into dune origin, longevity and migration. Detailed surface topography from GPS confirms height and width of dunes inferred earlier using ICESat. Internal layering of megadunes (imaged using ground-penetrating radar) shows sigmoidal layers of higher accumulation along the dune crests and windward faces. Dunes accrete new layers in the upwind direction. Radar layer structures, consisting of a 6- to 15-meter-thick sequence of accumulation layers separated by erosive or very low accretion glaze layers, are visible to at least 70 meters below the surface. Given an estimated mean accumulation of 20 - 30 kg/m2 over the dune region, each dune sequence represents approximately 250 years of time. High accretion occurs over roughly 1/3 of an active dune field surface. This implies that surfaces on the lee-side dune face spend between 150 and 200 years exposed to near-surface air and temperature variations before burial by the next advancing dune face. GPS ice motion measurements

  2. Drill cuttings mount formation study (United States)

    Teh, Su Yean; Koh, Hock Lye


    Oil, Gas and Energy sector has been identified as an essential driving force in the Malaysian Economic Transformation Programs (ETP). Recently confirmed discovery of many offshore oil and gas deposits in Malaysian waters has ignited new confidence in this sector. However, this has also spurred intense interest on safeguarding the health and environment of coastal waters in Malaysia from adverse impact resulting from offshore oil and gas production operation. Offshore discharge of spent drilling mud and rock cuttings is the least expensive and simplest option to dispose of large volumes of drilling wastes. But this onsite offshore disposal may have adverse environmental impacts on the water column and the seabed. It may also pose occupational health hazards to the workers living in the offshore platforms. It is therefore important to model the transport and deposition of drilling mud and rock cuttings in the sea to enable proper assessment of their adverse impacts on the environment and the workers. Further, accumulation of drill particles on the seabed may impede proper operation of pipelines on the seabed. In this paper, we present an in-house application model TUNA-PT developed to cater to local oil and gas industry needs to simulate the dispersion and mount formation of drill cuttings by offshore oil and gas exploration and production platforms. Using available data on Malaysian coastal waters, simulation analyses project a pile formation on the seabed with a maximum height of about 1 m and pile radius of around 30 to 50 m. Simulated pile heights are not sensitive to the heights of release of the cuttings as the sensitivity has been mitigated by the depth of water.

  3. MDF: Magnetic Particle Imaging Data Format

    CERN Document Server

    Knopp, Tobias; Bringout, Gael; Ahlborg, Mandy; Rahmer, Jürgen; Hofmann, Martin


    Magnetic particle imaging (MPI) is a tomographic method to determine the spatial distribution of magnetic nanoparticles. In this document a file format for the standardized storage of MPI data is introduced. The aim of the Magnetic Particle Imaging Data Format (MDF) is to provide a coherent way of exchanging MPI data acquired with different MPI scanners worldwide. The focus of the file format is on sequence parameters, raw measurement data, calibration data, and reconstruction data. The format is based on the hierarchical document format (HDF) in version 5 (HDF5).

  4. Heating tar sands formations while controlling pressure (United States)

    Stegemeier, George Leo [Houston, TX; Beer, Gary Lee [Houston, TX; Zhang, Etuan [Houston, TX


    Methods for treating a tar sands formation are described herein. Methods may include heating at least a section of a hydrocarbon layer in the formation from a plurality of heaters located in the formation. A pressure in the majority of the section may be maintained below a fracture pressure of the formation. The pressure in the majority of the section may be reduced to a selected pressure after the average temperature reaches a temperature that is above C. and is at or below pyrolysis temperatures of hydrocarbons in the section. At least some hydrocarbon fluids may be produced from the formation.

  5. Improving the Formatting Tools of CDS Invenio

    CERN Document Server

    Caffaro, J; Pu Faltings, Pearl


    CDS Invenio is the web-based integrated digital library system developed at CERN. It is a strategical tool that supports the archival and open dissemination of documents produced by CERN researchers. This paper reports on my Master’s thesis work done on BibFormat, a module in CDS Invenio, which formats documents metadata. The goal of this project was to implement a completely new formatting module for CDS Invenio. In this report a strong emphasis is put on the user-centered design of the new BibFormat. The bibliographic formatting process and its requirements are discussed. The task analysis and its resulting interaction model are detailed. The document also shows the implemented user interface of BibFormat and gives the results of the user evaluation of this interface. Finally the results of a small usability study of the formats included in CDS Invenio are discussed.

  6. Magnetic Assisted Colloidal Pattern Formation (United States)

    Yang, Ye

    Pattern formation is a mysterious phenomenon occurring at all scales in nature. The beauty of the resulting structures and myriad of resulting properties occurring in naturally forming patterns have attracted great interest from scientists and engineers. One of the most convenient experimental models for studying pattern formation are colloidal particle suspensions, which can be used both to explore condensed matter phenomena and as a powerful fabrication technique for forming advanced materials. In my thesis, I have focused on the study of colloidal patterns, which can be conveniently tracked in an optical microscope yet can also be thermally equilibrated on experimentally relevant time scales, allowing for ground states and transitions between them to be studied with optical tracking algorithms. In particular, I have focused on systems that spontaneously organize due to particle-surface and particle-particle interactions, paying close attention to systems that can be dynamically adjusted with an externally applied magnetic or acoustic field. In the early stages of my doctoral studies, I developed a magnetic field manipulation technique to quantify the adhesion force between particles and surfaces. This manipulation technique is based on the magnetic dipolar interactions between colloidal particles and their "image dipoles" that appear within planar substrate. Since the particles interact with their own images, this system enables massively parallel surface force measurements (>100 measurements) in a single experiment, and allows statistical properties of particle-surface adhesion energies to be extracted as a function of loading rate. With this approach, I was able to probe sub-picoNewton surface interactions between colloidal particles and several substrates at the lowest force loading rates ever achieved. In the later stages of my doctoral studies, I focused on studying patterns formed from particle-particle interaction, which serve as an experimental model of

  7. Benzene formation in electronic cigarettes.

    Directory of Open Access Journals (Sweden)

    James F Pankow

    Full Text Available The heating of the fluids used in electronic cigarettes ("e-cigarettes" used to create "vaping" aerosols is capable of causing a wide range of degradation reaction products. We investigated formation of benzene (an important human carcinogen from e-cigarette fluids containing propylene glycol (PG, glycerol (GL, benzoic acid, the flavor chemical benzaldehyde, and nicotine.Three e-cigarette devices were used: the JUULTM "pod" system (provides no user accessible settings other than flavor cartridge choice, and two refill tank systems that allowed a range of user accessible power settings. Benzene in the e-cigarette aerosols was determined by gas chromatography/mass spectrometry. Benzene formation was ND (not detected in the JUUL system. In the two tank systems benzene was found to form from propylene glycol (PG and glycerol (GL, and from the additives benzoic acid and benzaldehyde, especially at high power settings. With 50:50 PG+GL, for tank device 1 at 6W and 13W, the formed benzene concentrations were 1.9 and 750 μg/m3. For tank device 2, at 6W and 25W, the formed concentrations were ND and 1.8 μg/m3. With benzoic acid and benzaldehyde at ~10 mg/mL, for tank device 1, values at 13W were as high as 5000 μg/m3. For tank device 2 at 25W, all values were ≤~100 μg/m3. These values may be compared with what can be expected in a conventional (tobacco cigarette, namely 200,000 μg/m3. Thus, the risks from benzene will be lower from e-cigarettes than from conventional cigarettes. However, ambient benzene air concentrations in the U.S. have typically been 1 μg/m3, so that benzene has been named the largest single known cancer-risk air toxic in the U.S. For non-smokers, chronically repeated exposure to benzene from e-cigarettes at levels such as 100 or higher μg/m3 will not be of negligible risk.

  8. Benzene formation in electronic cigarettes. (United States)

    Pankow, James F; Kim, Kilsun; McWhirter, Kevin J; Luo, Wentai; Escobedo, Jorge O; Strongin, Robert M; Duell, Anna K; Peyton, David H


    The heating of the fluids used in electronic cigarettes ("e-cigarettes") used to create "vaping" aerosols is capable of causing a wide range of degradation reaction products. We investigated formation of benzene (an important human carcinogen) from e-cigarette fluids containing propylene glycol (PG), glycerol (GL), benzoic acid, the flavor chemical benzaldehyde, and nicotine. Three e-cigarette devices were used: the JUULTM "pod" system (provides no user accessible settings other than flavor cartridge choice), and two refill tank systems that allowed a range of user accessible power settings. Benzene in the e-cigarette aerosols was determined by gas chromatography/mass spectrometry. Benzene formation was ND (not detected) in the JUUL system. In the two tank systems benzene was found to form from propylene glycol (PG) and glycerol (GL), and from the additives benzoic acid and benzaldehyde, especially at high power settings. With 50:50 PG+GL, for tank device 1 at 6W and 13W, the formed benzene concentrations were 1.9 and 750 μg/m3. For tank device 2, at 6W and 25W, the formed concentrations were ND and 1.8 μg/m3. With benzoic acid and benzaldehyde at ~10 mg/mL, for tank device 1, values at 13W were as high as 5000 μg/m3. For tank device 2 at 25W, all values were ≤~100 μg/m3. These values may be compared with what can be expected in a conventional (tobacco) cigarette, namely 200,000 μg/m3. Thus, the risks from benzene will be lower from e-cigarettes than from conventional cigarettes. However, ambient benzene air concentrations in the U.S. have typically been 1 μg/m3, so that benzene has been named the largest single known cancer-risk air toxic in the U.S. For non-smokers, chronically repeated exposure to benzene from e-cigarettes at levels such as 100 or higher μg/m3 will not be of negligible risk.

  9. New Office Software course format!

    CERN Multimedia

    HR Department

    Always keen to anticipate your  training needs, the Technical Training service is pleased to propose two new Office Software course formats: “Focus on... ”:  On a monthly basis we will propose a theme such as “Sharepoint Collaboration Workspace” or “Word 2007” or “charts”, etc.  You will be invited to send us in advance your questions regarding the proposed topic and register  for the course through our Training Catalogue. During the session, our trainer will answer all the questions received and participants will increase their knowledge thanks to the solutions discussed for everyone. The course will last two hours, from 9-00 to 11-00 a.m. - with open questions on the proposed topic at the end.   “Office software Individual coaching”: If one or more specific topics are causing you sleepless nights, you can get the help of our trainer who will ...

  10. New Office Software course format!

    CERN Multimedia

    HR Department


    Always keen to anticipate your  training needs, the Technical Training service is pleased to propose two new Office Software course formats: “Focus on... ”:  On a monthly basis we will propose a theme such as “Sharepoint Collaboration Workspace” or “Word 2007” or “charts”, etc.  You will be invited to send us in advance your questions regarding the proposed topic and register  for the course through our Training Catalogue. During the session, our trainer will answer all the questions received and participants will increase their knowledge thanks to the solutions discussed for everyone. The course will last two hours, from 9-00 to 11-00 a.m. - with open questions on the proposed topic at the end.   “Office software Individual coaching”: If one or more specific topics are causing you sleepless nights, you can get the help of our trainer who will ...

  11. Testing models for structure formation. (United States)

    Kaiser, N.

    The author reviews a number of tests of theories for structure formation. Large-scale flows and IRAS galaxies indicate a high density parameter Ω ≅ 1, in accord with inflationary predictions, but it is not clear how this meshes with the uniformly low values obtained from virial analysis on scales ≡1 Mpc. Gravitational distortion of faint galaxies behind clusters allows one to construct maps of the mass surface density, and this should shed some light on the large vs. small-scale Ω discrepancy. Power spectrum analysis reveals too red a spectrum on scales λ ≡ 10 - 100 h-1Mpc, but the gaussian fluctuation hypothesis appears to be in good shape. These results suggest that the problem for CDM lies not in the very early universe but in the assumed matter content. The power spectrum problem can be solved by invoking a cocktail of mixed dark matter. However, if gravitational lensing fails to reveal extended dark mass around clusters then we may be forced to explore more radical possibilities for the dark matter.

  12. Markedet for TV-formater

    Directory of Open Access Journals (Sweden)

    Thomas Wedell-Wedellsborg


    Full Text Available Fremvæksten af et formaliseret internationalt marked for TV-formater har i høj grad forårsaget en stille revolution i TV-branchen igennem de sidste 10-15 år. En revolution, fordi udenlandsk skabte underholdnings- programmer har vundet indpas i primetime overalt på sendefladen i form af programmer som f.eks. Popstars, Idols, Hvem vil være millionær, Jeopardy, Par på prøve, Robinson, Big Brother og Room Service. En stille revolution, fordi dette faktum ikke har fået tildelt synderlig megen opmærksomhed uden for TV-branchens egne rammer. Ser man bort fra den juridiske litteratur, hvor formaterne har fået ganske megen opmærk- somhed i forbindelse med ophavsretslige spørgsmål, eksisterer der reelt meget få akademiske studier af formatmarkedet. Denne artikels mål er derfor at give en indledende, medieøkonomisk orienteret beskrivelse af formatmarkedet, dets aktører og dets virkemåde. Artiklen er baseret på mit speciale ‘Markedet for TV-formater’ (under udarbejdelse.

  13. Formatting biopolymers using adjustable nanoconfinement (United States)

    Berard, Daniel; Shayegan, Marjan; Michaud, Francois; Henkin, Gil; Scott, Shane; Leslie, Sabrina

    Sensitive visualization and conformational control of long, delicate biopolymers present critical challenges to emerging biotechnologies and biophysical studies. Next-generation nanofluidic manipulation platforms strive to maintain the structural integrity of genomic DNA prior to analysis but can face challenges in device clogging, molecular breakage, and single-label detection. We address these challenges by integrating the Convex Lens-induced Confinement (CLiC) technique with a suite of nanotopographies embedded within thin-glass nanofluidic chambers. We gently load DNA polymers into open-face nanogrooves in linear, concentric circular, and ring array formats and perform imaging with single-fluorophore sensitivity. We use ring-shaped nanogrooves to access and visualize confinement-enhanced self-ligation of long DNA polymers. We use concentric circular nanogrooves to enable hour-long observations of polymers at constant confinement in a geometry which eliminates the confinement gradient which causes drift and can alter molecular conformations and interactions. Taken together, this work opens doors to myriad biophysical studies and biotechnologies which operate on the nanoscale.

  14. Dune formation under bimodal winds. (United States)

    Parteli, Eric J R; Durán, Orencio; Tsoar, Haim; Schwämmle, Veit; Herrmann, Hans J


    The study of dune morphology represents a valuable tool in the investigation of planetary wind systems--the primary factor controlling the dune shape is the wind directionality. However, our understanding of dune formation is still limited to the simplest situation of unidirectional winds: There is no model that solves the equations of sand transport under the most common situation of seasonally varying wind directions. Here we present the calculation of sand transport under bimodal winds using a dune model that is extended to account for more than one wind direction. Our calculations show that dunes align longitudinally to the resultant wind trend if the angle(w) between the wind directions is larger than 90 degrees. Under high sand availability, linear seif dunes are obtained, the intriguing meandering shape of which is found to be controlled by the dune height and by the time the wind lasts at each one of the two wind directions. Unusual dune shapes including the "wedge dunes" observed on Mars appear within a wide spectrum of bimodal dune morphologies under low sand availability.

  15. Spheromak formation studies in SSPX

    Energy Technology Data Exchange (ETDEWEB)

    Hill, D N; Bulmer, R H; Cohen, B L; Hooper, E B; LoDestro, L L; Mattor, N; McLean, H S; Moller, J; Pearlstein, L D; Ryutov, D D; Stallard, B W; Wood, R D; Woodruff, S; Holcomb, C T; Jarboe, T; Sovinec, C R; Wang, Z; Wurden, G


    We present results from the Sustained Spheromak Physics Experiment (SSPX) at LLNL, which has been built to study energy confinement in spheromak plasmas sustained for up to 2 ms by coaxial DC helicity injection. Peak toroidal currents as high as 600kA have been obtained in the 1m dia. (0.23m minor radius) device using injection currents between 200-400kA; these currents generate edge poloidal fields in the range of 0.2-0.4T. The internal field and current profiles are inferred from edge field measurements using the CORSICA code. Density and impurity control is obtained using baking, glow discharge cleansing, and titanium gettering, after which long plasma decay times ({tau} {ge} 1.5ms) are observed and impurity radiation losses are reduced from {approx}50% to <20% of the input energy. Thomson scattering measurements show peaked electron temperature and pressure profiles with T{sub e} (0){approx}120eV and {beta}{sub e}{approx}7%. Edge field measurements show the presence of n=1 modes during the formation phase, as has been observed in other spheromaks. This mode dies away during sustainment and decay so that edge fluctuation levels as low as 1% have been measured. These results are compared with numerical simulations using the NIMROD code.

  16. Rowing sportswomen motor actions formation

    Directory of Open Access Journals (Sweden)

    Volodymyr Bogush


    Full Text Available Purpose: to study the formation of motor action sportswomen different ages depending on the level of sportsmanship. Material and Methods: girls which are specialized in a boat-racing in age groups 13–14 years inspected, 15–16 years, 17–18 years, in every group was for 20–25 persons, in all 72 sportswomen. Motive actions were probed on the method of measuring of training effect developed by us an action, and also the functional state was determined by methods: measuring of sensorimotor reaction is on sound and light irritants, speed of current of air, exactness of implementation of the set muscular effort. Results: testing showed the dynamics of forming motive, namely technique of mastering of receptions and actions, reliability, presence of errors, efficiency of active voice of consciousness in correct implementation of motion in a biomechanics relation. Conclusions: application of this method in the process of sporting preparation will allow to define quality of mastering of technique of the proper motive actions, forming of abilities, subsequent learning and becoming of more difficult motive skills

  17. Characterization of formation damage by particulate processes

    Energy Technology Data Exchange (ETDEWEB)

    Arcia, E.M. (EPRY, Maraven S.A., Caracas (Venezuela)); Civan, F. (Oklahoma Univ., Norman, OK (United States))


    Reduction of injectivity of wells by formation damage can be of considerable magnitude during waterflooding of oil reservoirs. A model-assisted analysis of formation damage in the S and S3 facies of the Eocene B-inferior formation in Ceuta field, Lake Maracaibo, western Venezuela is carried out. Details are presented of materials, experimental system and procedures. The governing mechanisms and the estimated values of the phenomenological rate constants are determined using the formation damage model of Ohen and Civan. A skin factor chart is constructed based on this information as a practical measure of the formation damage potential. Due to the high content of carbonaceous and siliceous type authigenic materials in the Ceuta field, core samples are highly sensitive to brine, rendering the pH towards alkaline during waterflooding, resulting in dispersion of particles and reduction in formation permeability. 18 refs., 13 figs., 3 tabs.

  18. Autonomous Formations of Multi-Agent Systems (United States)

    Dhali, Sanjana; Joshi, Suresh M.


    Autonomous formation control of multi-agent dynamic systems has a number of applications that include ground-based and aerial robots and satellite formations. For air vehicles, formation flight ("flocking") has the potential to significantly increase airspace utilization as well as fuel efficiency. This presentation addresses two main problems in multi-agent formations: optimal role assignment to minimize the total cost (e.g., combined distance traveled by all agents); and maintaining formation geometry during flock motion. The Kuhn-Munkres ("Hungarian") algorithm is used for optimal assignment, and consensus-based leader-follower type control architecture is used to maintain formation shape despite the leader s independent movements. The methods are demonstrated by animated simulations.

  19. Star Formation Rate Maps of Nearby Galaxies (United States)

    Stone, Frances H.; Pooley, David


    A key component of many extragalactic studies is the correlation of a galaxy’s overall star formation rate with a particular type of astronomical object (like supernovae or luminous X-ray sources). While these correlations have allowed for considerable progress in understanding the nature, formation, and diversity of these objects, the overall star formation rate is a rather blunt instrument. Star formation is not uniform across a galaxy, and maps of local star formation rates can be made. A well calibrated method by Leroy et al. (2007) employs a weighted combination of far ultraviolet (FUV) data from the Galaxy Evolution Explorer Telescope (GALEX) and 24-micron data from the Spitzer Space Telescope. We have applied this method to archival images of several nearby galaxy as part of an ongoing effort to build a star formation rate atlas of thousands of nearby galaxies. We present results for some of the most active star forming galaxies we have analyzed so far.

  20. Electromagnetic Formation Flying with Eccentric Reference Orbits


    Palacios, L.; Ceriotti, Matteo; Radice, Gianmarco


    Over the last decade, a considerable amount of research work has been done in the area of spacecraft formation flight, with particular emphasis on control techniques using thruster-based systems. Nevertheless, thrusters require propellant to work and this limit the lifetime of the mission. Electromagnetic Formation Flight (EMFF) is presented in this paper as a fuel-less strategy to control spacecraft formations by means of electromagnets. In EMFF, spacecraft can be equipped with one or more c...

  1. Massive Star and Star Cluster Formation


    Tan, Jonathan C.


    I review the status of massive star formation theories: accretion from collapsing, massive, turbulent cores; competitive accretion; and stellar collisions. I conclude the observational and theoretical evidence favors the first of these models. I then discuss: the initial conditions of star cluster formation as traced by infrared dark clouds; the cluster formation timescale; and comparison of the initial cluster mass function in different galactic environments.



    Kinderis, Remigijus; Jucevičius, Giedrius


    The article presents analysis of the definition of strategic alliances, the analysis of alliance and the research of a strategic alliance concept; furthermore, it focuses on the contingent hierarchy of alliances. The motives of strategic alliances formation, their categories, groups and benefit for business have been revealed in this article. Special attention is paid to the process of strategic alliance formation and the analysis of factors that influence the formation of strategic alliances...

  3. Theoretical Aspects of Enterprise Business Strategy Formation


    Valentinavičius, Stasys


    The paper presents interpretations of business strategy concept and analyses strategy planning and formation, models. The concept of business strategy is revised and formulated considering various authors approaches. Analysis of business strategy formation process – steps of development and management, selection of strategy type – is based on presented strategy planning models. The aspects of enterprise business and investment strategy formation, coordination and valuation are analysed. The s...

  4. Complications and mortality following stoma formation.


    Harris, D. A.; Egbeare, D.; Jones, S.; Benjamin, H.; Woodward, A; Foster, M E


    INTRODUCTION: As stoma formation is thought to be declining, we performed a study to evaluate the rate of stoma formation and the impact on stoma complication rates, together with risk factors for complications. PATIENTS AND METHODS: Stoma incidence, individual complications and mortality rates were retrieved from a stoma nurse database of 345 stomas created over an 8-year period. RESULTS: Stoma formation increased over the study period, although the incidence of complications declined. Stoma...

  5. Star Formation in IC 348 (United States)

    Herbst, W.


    A review of work on the small, compact, nearby young cluster IC 348 is given. This region is particularly important because it is well surveyed at a variety of wavelengths and intermediate in nature between dense clusters and loose associations. Its earliest type star is B5 and it contains a few hundred stellar members as well as some brown dwarfs, protostars, Herbig-Haro objects and starless sub-mm cores. The total mass of its components is ˜90 M_⊙, most of which is in the form of pre-main sequence stars. Perhaps the biggest challenge to work on the cluster is the relatively high and variable extinction (A_v=3D1-7 mag). Studies to date have provided particularly valuable insights into the initial mass function, disk lifetimes, stellar rotation properties, X-ray properties, outflows and substructure of the cluster. Results on the stellar component include the following: 1) the initial mass function matches that for field stars in the stellar and brown dwarf regimes, 2) the fraction of stars with disks is probably normal for the cluster's age, 3) the rotation properties match those of the Orion Nebula Cluster and are significantly different, in the sense of slower rotation, than NGC 2264, 4) the X-ray properties of the stars appear normal for T Tauri stars. There is a ridge of high extinction that lies ˜10 arcmin (0.9 pc in projection) to the southwest of IC 348 and contains about a dozen Class 0 and I protostars as well as some Herbig Haro objects and sub-mm cores. This region, which also contains the "Flying Ghost Nebula" and the well-studied object HH 211, clearly signals that star formation in this part of the Perseus dark clouds is not yet finished. An extensive kinematical study involving both proper motions and radial velocities for the 400 members of the cluster would be most desirable.

  6. Treating tar sands formations with dolomite (United States)

    Vinegar, Harold J.; Karanikas, John Michael


    A method for treating a karsted formation containing heavy hydrocarbons and dolomite includes providing heat to at least part of one or more karsted layers in the formation from one or more heaters located in the karsted layers. A temperature in at least one of the karsted layers is allowed to reach a decomposition temperature of dolomite in the formation. The dolomite is allowed to decompose and at least some hydrocarbons are produced from at least one of the karsted layers of the formation.

  7. New Particle Formation Study Final Campaign Report

    Energy Technology Data Exchange (ETDEWEB)

    Smith, JN; McMurry, PH [University of Minnesota


    The scientific foci of the New Particle Formation Study were the formation and evolution of atmospheric aerosols and the impacts of newly formed particles on cloud processes. Specifically, we planned to: (1) to identify the species and mechanisms responsible for the initial steps of new particle formation, i.e., the formation of thermodynamically stable clusters; (2) investigate the role of acid-base chemistry in new particle growth through measurements of ammonia and amines as well as organic and inorganic acids in both atmospheric nanoparticles and the gas phase; (3) investigate the contribution of other surface area or volume-controlled processes to nanoparticle formation and growth; (4) create a comprehensive dataset related to new particle formation and growth that can be used as input for our own thermodynamic models as well as the modeling efforts by our Department of Energy (DOE) Aerosol Life Cycle working group collaborators; (5) characterize the increase of the number and activity of cloud condensation nuclei (CCN) due to particle formation and growth; (6) determine the regional extent of new particle formation to address the role that atmospheric transport plays in determining the impacts, if any, of new particle formation on cloud number and properties.



    イシイ, セイゴ; ナリタ, ヒデキ; マエノ, ノリカズ; Seigo, ISHII; Hideki, NARITA; Norikazu, MAENO


    Bubble formation experiments were conducted for snow composed of ice spheres 303μm in diameter at various temperatures and applied pressures. By measuring volumes of closed-off bubbles at various densities, the bubble formation density (ρ_f) and the bubble close-off density (ρ_c) were obtained. ρ_f, that is the density at which bubble formation begins, decreased with lowering temperature or pressure. On the other hand, ρ_c, that is the density at which bubble formation finishes, increased wit...

  9. Characteristics of improved formative assessment practice

    National Research Council Canada - National Science Library

    Andersson, Catarina; Palm, Torulf


    An earlier study showed that the changes in teachers’ classroom practice, after participation in a professional development program in formative assessment, significantly improved student achievement in mathematics...

  10. Star formation inside a galactic outflow. (United States)

    Maiolino, R; Russell, H R; Fabian, A C; Carniani, S; Gallagher, R; Cazzoli, S; Arribas, S; Belfiore, F; Bellocchi, E; Colina, L; Cresci, G; Ishibashi, W; Marconi, A; Mannucci, F; Oliva, E; Sturm, E


    Recent observations have revealed massive galactic molecular outflows that may have the physical conditions (high gas densities) required to form stars. Indeed, several recent models predict that such massive outflows may ignite star formation within the outflow itself. This star-formation mode, in which stars form with high radial velocities, could contribute to the morphological evolution of galaxies, to the evolution in size and velocity dispersion of the spheroidal component of galaxies, and would contribute to the population of high-velocity stars, which could even escape the galaxy. Such star formation could provide in situ chemical enrichment of the circumgalactic and intergalactic medium (through supernova explosions of young stars on large orbits), and some models also predict it to contribute substantially to the star-formation rate observed in distant galaxies. Although there exists observational evidence for star formation triggered by outflows or jets into their host galaxy, as a consequence of gas compression, evidence for star formation occurring within galactic outflows is still missing. Here we report spectroscopic observations that unambiguously reveal star formation occurring in a galactic outflow at a redshift of 0.0448. The inferred star-formation rate in the outflow is larger than 15 solar masses per year. Star formation may also be occurring in other galactic outflows, but may have been missed by previous observations owing to the lack of adequate diagnostics.

  11. Heating systems for heating subsurface formations

    Energy Technology Data Exchange (ETDEWEB)

    Nguyen, Scott Vinh [Houston, TX; Vinegar, Harold J [Bellaire, TX


    Methods and systems for heating a subsurface formation are described herein. A heating system for a subsurface formation includes a sealed conduit positioned in an opening in the formation and a heat source. The sealed conduit includes a heat transfer fluid. The heat source provides heat to a portion of the sealed conduit to change phase of the heat transfer fluid from a liquid to a vapor. The vapor in the sealed conduit rises in the sealed conduit, condenses to transfer heat to the formation and returns to the conduit portion as a liquid.

  12. Towards the Rosetta Stone of planet formation

    Directory of Open Access Journals (Sweden)

    Schmidt T.O.B.


    Full Text Available Transiting exoplanets (TEPs observed just ~10 Myrs after formation of their host systems may serve as the Rosetta Stone for planet formation theories. They would give strong constraints on several aspects of planet formation, e.g. time-scales (planet formation would then be possible within 10 Myrs, the radius of the planet could indicate whether planets form by gravitational collapse (being larger when young or accretion growth (being smaller when young. We present a survey, the main goal of which is to find and then characterise TEPs in very young open clusters.

  13. Cellular chain formation in Escherichia coli biofilms

    DEFF Research Database (Denmark)

    Vejborg, Rebecca Munk; Klemm, Per


    In this study we report on a novel structural phenotype in Escherichia coli biofilms: cellular chain formation. Biofilm chaining in E. coli K-12 was found to occur primarily by clonal expansion, but was not due to filamentous growth. Rather, chain formation was the result of intercellular......; type I fimbriae expression significantly reduced cellular chain formation, presumably by steric hindrance. Cellular chain formation did not appear to be specific to E coli K-12. Although many urinary tract infection (UTI) isolates were found to form rather homogeneous, flat biofilms, three isolates...

  14. Ozone suppression by dew formation (United States)

    Takenaka, N.; Shimazaki, W.; Sadanaga, Y.; Bandow, H.


    the presentation, we will report the effect of dew formation on the ozone concentration by using experimental results, monitoring results and calculation results by a BOX model.

  15. Implementation of Formative Assessment in the Classroom (United States)

    Edman, Elaina; Gilbreth, Stephen G.; Wynn, Sheila


    This report details the work defined by a doctoral team looking at the literacy and implementation of formative assessment in classrooms in Southwest Missouri. The mission of this project was to identify the formative assessment literacy levels and the degree of classroom implementation of these strategies in districts and the resulting…

  16. A Comparison of Conjoint Analysis Response Formats (United States)

    Kevin J. Boyle; Thomas P. Holmes; Mario F. Teisl; Brian Roe


    A split-sample design is used to evaluate the convergent validity of three response formats used in conjoint analysis experiments. WC investigate whether recoding rating data to rankings and choose-one formats, and recoding ranking data to choose one. result in structural models and welfare estimates that are statistically indistinguishable from...

  17. Designing Crowdcritique Systems for Formative Feedback (United States)

    Easterday, Matthew W.; Rees Lewis, Daniel; Gerber, Elizabeth M.


    Intelligent tutors based on expert systems often struggle to provide formative feedback on complex, ill-defined problems where answers are unknown. Hybrid crowdsourcing systems that combine the intelligence of multiple novices in face-to-face settings might provide an alternate approach for providing intelligent formative feedback. The purpose of…

  18. Distributed formation control for autonomous robots

    NARCIS (Netherlands)

    Garcia de Marina Peinado, Hector Jesús


    This thesis addresses several theoretical and practical problems related to formation-control of autonomous robots. Formation-control aims to simultaneously accomplish the tasks of forming a desired shape by the robots and controlling their coordinated collective motion. This kind of robot

  19. Nature and nurture in galaxy formation simulations

    NARCIS (Netherlands)

    Haas, Marcel Richard


    We study several aspects of the formation of galaxies, using numerical simulations. We investigate the influence of about thirty different sub-grid physics recipes for cooling, star formation, supernova feedback, AGN feedback etc. on the resulting galaxy populations with large SPH simulations. We

  20. General certification procedure of formation organizations

    CERN Document Server

    Int. At. Energy Agency, Wien


    This document presents the procedure dealing with the certification of formation organizations dispensing the formation and the risks prevention to the personnel of A or B category in nuclear facilities. This certification proves the organization ability to satisfy the ''F'' specification of the CEFRI. (A.L.B.)

  1. Probes of Cosmic Star Formation History

    Indian Academy of Sciences (India)

    I summarize X-ray diagnostic studies of cosmic star formation history in terms of evolutionary schemes for X-ray binary evolution in normal galaxies with evolving star formation. Deep X-ray imaging studies by Chandra and XMM-Newton are now beginning to constrain both the X-ray luminosity evolution of galaxies and the ...

  2. Island Formation: Constructing a Coral Island (United States)

    Austin, Heather; Edd, Amelia


    The process of coral island formation is often difficult for middle school students to comprehend. Coral island formation is a dynamic process, and students should have the opportunity to experience this process in a synergistic context. The authors provide instructional guidelines for constructing a coral island. Students play an interactive role…

  3. Stone Formation in the Infected Pediatric Enterocystoplasty

    NARCIS (Netherlands)

    R.B. Mathoera (Rejiv)


    markdownabstract__Abstract__ Proteus mirabilis is one of the most frequent bacterial agents that can induce infection stone formation by urease production. In recent years the influence of Proteus mirabilis on stone formation in enterocystoplasties has been primarily related to the presence of

  4. Bone formation: roles of genistein and daidzein (United States)

    Bone remodeling consists of a balance between bone formation by osteoblasts and bone resorption by osteoclasts. Osteoporosis is the result of increased bone resorption and decreased bone formation causing a decreased bone mass density, loss of bone microarchitecture, and an increased risk of fractu...

  5. Formation and evolution of compact binaries

    NARCIS (Netherlands)

    Sluijs, Marcel Vincent van der


    In this thesis we investigate the formation and evolution of compact binaries. Chapters 2 through 4 deal with the formation of luminous, ultra-compact X-ray binaries in globular clusters. We show that the proposed scenario of magnetic capture produces too few ultra-compact X-ray binaries to explain

  6. Salicylic acid electrooxidation. A surface film formation

    Energy Technology Data Exchange (ETDEWEB)

    Baturova, M.D.; Vedenjapin, A.; Baturova, M.M. [N.D. Zelinsky Inst. of Organic Chemistry, Russian Academy of Sciences, Moscow (Russian Federation); Weichgrebe, D.; Danilova, E.; Rosenwinkel, K.H. [Univ. of Hannover, Inst. of Water Quality and Waste Management Hannover (Germany); Skundin, A. [A.N. Frumkin Inst. of Electrochemistry, Russian Academy of Sciences, Moscow (Russian Federation)


    A possibility to use electrochemical treatment for salicylic acid (SA) removal from waste water was studied. It was found that SA can be oxidized at platinum anode with formation of harmless products. Features of anodic process, in particular, formation of solid film on anode surface as well as properties of the film were investigated. (orig.)

  7. Endocannabinoid signaling is critical for habit formation

    Directory of Open Access Journals (Sweden)

    Monica R. F Hilário


    Full Text Available Extended training can induce a shift in behavioral control from goal-directed actions, which are governed by action-outcome contingencies and sensitive to change in the expected value of the outcome, to habits which are less dependent on action-outcome relations and insensitive to changes in outcome value. Previous studies in rats have shown that interval schedules of reinforcement favor habit formation while ratio schedules favor goal-directed behavior. However, the molecular mechanisms underlying habit formation are not well understood. Endocannabinoids, which can function as retrograde messengers acting through presynaptic CB1 receptors, are highly expressed in the dorsolateral striatum, a key region involved in habit formation. Using a reversible devaluation paradigm, we confirmed that in mice random interval schedules also favor habit formation compared with random ratio schedules. We also found that training with interval schedules resulted in a preference for exploration of a novel lever, whereas training with ratio schedules resulted in less generalization and more exploitation of the reinforced lever. Furthermore, mice carrying either a heterozygous or a homozygous null mutation of the cannabinoid receptor type I (CB1 showed reduced habit formation and enhanced exploitation. The impaired habit formation in CB1 mutant mice cannot be attributed to chronic developmental or behavioral abnormalities because pharmacological blockade of CB1 receptors specifically during training also impairs habit formation. Taken together our data suggest that endocannabinoid signaling is critical for habit formation.

  8. Intrahepatic Biliary Cystadenoma With Intracystic Gallstone Formation (United States)

    Lei, Shize; Domenico, Don R.


    Biliary cystadenoma is a rare tumor of the liver. We describe a biliary cystadenoma of the left lobe of the liver with intracystic gallstone formation. This is the first report of stone formation in biliary cystadenoma of the liver. PMID:8155592

  9. Computer-based feedback in formative assessment

    NARCIS (Netherlands)

    van der Kleij, Fabienne


    Formative assessment concerns any assessment that provides feedback that is intended to support learning and can be used by teachers and/or students. Computers could offer a solution to overcoming obstacles encountered in implementing formative assessment. For example, computer-based assessments

  10. A UNIMARC Bibliographic Format Database for ABCD (United States)

    Megnigbeto, Eustache


    Purpose: ABCD is a web-based open and free software suite for library management derived from the UNESCO CDS/ISIS software technology. The first version was launched officially in December 2009 with a MARC 21 bibliographic format database. This paper aims to detail the building of the UNIMARC bibliographic format database for ABCD.…

  11. The Formation of Mini-Neptunes (United States)

    Venturini, Julia; Helled, Ravit


    Mini-Neptunes seem to be common planets. In this work we investigate the possible formation histories and predicted occurrence rates of mini-Neptunes, assuming that the planets form beyond the iceline. We consider pebble and planetesimal accretion accounting for envelope enrichment and two different opacity conditions. We find that the formation of mini-Neptunes is a relatively frequent output when envelope enrichment by volatiles is included, and that there is a “sweet spot” for mini-Neptune formation with a relatively low solid accretion rate of ˜10-6 M ⊕ yr-1. This rate is typical for low/intermediate-mass protoplanetary disks and/or disks with low metallicities. With pebble accretion, envelope enrichment and high opacity favor the formation of mini-Neptunes, with more efficient formation at large semimajor axes (˜30 au) and low disk viscosities. For planetesimal accretion, such planets can also form without enrichment, with the opacity being a key aspect in the growth history and favorable formation location. Finally, we show that the formation of Neptune-like planets remains a challenge for planet formation theories.

  12. Achievement Goal Orientations and Identity Formation Styles (United States)

    Kaplan, Avi; Flum, Hanoch


    The present article points to shared underlying theoretical assumptions and central processes of a prominent academic motivation perspective--achievement goal theory--and recent process perspectives in the identity formation literature, and more specifically, identity formation styles. The review highlights the shared definition of achievement…

  13. Curriculum Formation: A Case Study from History (United States)

    Shay, Suellen


    Drawing on the work of Bernstein and Maton and using a case-study approach, this study explores the formation of an undergraduate history curriculum at the University of Cape Town. This article focuses on two periods of curriculum formation referred to as history as canon and history as social science. With respect to these two curriculum periods…

  14. The Political Dilemmas of Formative Assessment (United States)

    Dorn, Sherman


    The literature base on using formative assessment for instructional and intervention decisions is formidable, but the history of the practice of formative assessment is spotty. Even with the pressures of high-stakes accountability, its definition is fuzzy, its adoption is inconsistent, and the prognosis for future use is questionable. A historical…

  15. Gas-Phase Infrared; JCAMP Format (United States)

    SRD 35 NIST/EPA Gas-Phase Infrared; JCAMP Format (PC database for purchase)   This data collection contains 5,228 infrared spectra in the JCAMP-DX (Joint Committee for Atomic and Molecular Physical Data "Data Exchange") format.

  16. Teacher Perspectives About Using Formative Assessment

    DEFF Research Database (Denmark)

    Evans, Robert Harry; Clesham, Rose; Dolin, Jens


    trying project-specific formative assessment methods in Czech Republic. Analyses are done through case studies and interviews. The final part of the chapter looks at teacher perspectives while using an Internet-based application to facilitate formative assessment. The teacher use of the application...... and their feedback about its utility are discussed....

  17. User-Friendly Materials and Alternate Formats. (United States)

    Southwest Educational Development Laboratory, Austin, TX. National Center for the Dissemination of Disability Research.

    This booklet offers guidance for increasing the dissemination and utilization of information (especially federally funded disability research) through development of user-friendly materials and alternate formats. Individual sections address: the distinction between alternate formats and accessibility, suggested policies to help define approaches…

  18. Identity formation in the New Testament


    Markus Cromhout


    This article is a review of the book entitled Identity Formation in the New Testament (edited by Bengt Holmberg and Mikael Winninge, Mohr Siebeck, Tübingen, 2008). It is a collection of various articles using intertextuality, literary theory (and social identity approaches), gender studies and postcolonial theory when investigating identity formation in the New Testament.

  19. Formative Assessment in the High School IMC (United States)

    Edwards, Valerie A.


    In this article, the author discusses how she uses formative assessments of information literacy skills in the high school IMC. As a result of informal observation and conversations with individual students--a form of formative assessment itself--the author learned that students were not using indexes to locate relevant information in nonfiction…

  20. Pedagogical Formation Education via Distance Education (United States)

    Ozcan, Deniz; Genc, Zeynep


    The purpose of this research is to identify the perceptions of the efficacy of curriculum development on the part of pedagogical formation students, their views regarding their professional attitudes, and their attitudes towards the pedagogical formation education they receive via distance education. The study sample includes 438 Near East…

  1. An introduction to the ENDF formats

    Energy Technology Data Exchange (ETDEWEB)



    The ENDF Evaluated Nuclear Data Formats are used all over the world to encode nuclear data evaluations for use in research and nuclear technology. This report is an introduction to the formats and how they are used in modern compilations of nuclear data.

  2. Network Formation under the Threat of Disruption

    NARCIS (Netherlands)

    Hoyer, B.|info:eu-repo/dai/nl/32205446X


    The studies in this thesis are focused on the impact the presence of a network disruptor has on network formation models. In particular, we build two theoretical models to study the effect of network disruption on network formation and test the effect network disruption has on equilibrium selection

  3. A Decentralized Approach to Formation Flight Routing

    NARCIS (Netherlands)

    Visser, H.G.; Lopes dos Santos, Bruno F.; Verhagen, C.M.A.


    This paper describes the development of an optimization-based cooperative planning system for the efficient routing and scheduling of flight formations. This study considers the use of formation flight as a means to reduce the overall fuel consumption of civil aviation in long-haul operations. It

  4. Anther Wall Formation in Solanaceae Species (United States)



    Anther wall formation was studied in 32 species belonging to 27 genera of Solanaceae. Dicotyledonous and basic types of wall formation were observed, as well as several deviations due to subsequent periclinal divisions in the layers formed (middle layers and sometimes the endothecium). One type of wall formation was observed in each species. Some genera are uniform in their type of wall formation, while others are heterogeneous; a similar situation was observed at the tribal level. Summarizing all reported information on anther wall formation in the Solanaceae, 64 % of species show the basic type, while the remaining 36 % show the dicotyledonous type. Thus, neither type predominates, and no single type characterizes genera, tribes or the entire family. PMID:12451025

  5. Teacher perspectives about using formative assessment

    DEFF Research Database (Denmark)

    Evans, Robert Harry; Dolin, Jens; Nielsen, Jan Alexis


    Abstract. This chapter examines three different classroom teacher perspectives when using ASSIST-ME project formative assessment methods as described in the introductory chapter. The first ‘teacher perspective' is about changes in teacher self-efficacies while using formative assessment methods...... as monitored by a pre and post teacher questionnaire. Teachers who tried the unfamiliar formative methods of assessment (see introductory book chapter for these methods) as well as their colleagues who did not were surveyed. The second ‘teacher perspective’ examines changes in teachers' subjective theories...... while trying project specific formative assessment methods in the Czech Republic. Analyses are done through case studies and interviews. The final part of the chapter looks at teacher perspectives while using an internet-based application to facilitate formative assessment. Teacher use...

  6. XML-based DICOM data format. (United States)

    Yu, Cong; Yao, Zhihong


    To enhance the readability, improve the structure, and facilitate the sharing of digital imaging and communications in medicine (DICOM) files, this research proposed one kind of XML-based DICOM data format. Because XML Schema offers great flexibility for expressing constraints on the content model of elements, we used it to describe the new format, thus making it consistent with the one originally defined by DICOM. Meanwhile, such schemas can be used in the creation and validation of the XML-encoded DICOM files, acting as a standard for data transmission and sharing on the Web. Upon defining the new data format, we started with representing a single data element and further improved the whole data structure with the method of modularization. In contrast to the original format, the new one possesses better structure without loss of related information. In addition, we demonstrated the application of XSLT and XQuery. All of the advantages mentioned above resulted from this new data format.

  7. Unified mask data formats for EB writers (United States)

    Kuriyama, Koki; Suzuki, Toshio; Hirumi, Junji; Yoshioka, Nobuyuki; Hojo, Yutaka; Kawase, Yuichi; Hara, Shigehiro; Hoga, Morihisa; Watanabe, Satoshi W.; Inoue, Masa; Kawase, Hidemuchi; Kamimoto, Tomoko


    Mask data preparation is a complicated process because many kinds of pattern files and jobdeck files flow into mask manufacturers. This situation has a significant impact on data preparation operations especially in mask manufacturers. In this paper, we propose a solution to this problem: use of unified mask data formats for EB writers and a model of data preparation flow from a device manufacturer to an EB writer. The unified formats consist of pattern data format named "NEO", and mask layout format named "MALY". NEO is a stream format which retains upper compatibility to GDSII and has higher compression rate than GDSII. NEO is intended to be a general input format of Variable-Shaped-Beam (VSB) mask writers in principle, not particularly designed for any specific equipment or software. Data conversion process between mask writers being taken into account, NEO requires some constraints for VSB mask writers, such as removal of overlapping figures. Due to many differences in jobdeck syntax and functions among mask writers, it is a complicated task to edit or modify a jobdeck, and convert it into another format. MALY is a text-based format whose purpose is to standardize mask layout information among mask writers. This unification of mask layout information optimized for EB writers is expected to reduce workload of mask data preparation significantly. Besides the information described in MALY, some other information specific to the target EB writer, such as drawing parameters, has to be prepared separately. This paper illustrates a model of data flow and benefits of using these unified formats. The format and the data flow are effective in reducing data handling cost, providing flexible data handling solution. Applying the handling flow using NEO and MALY would result in reducing the load on mask manufacturers. Moreover, device manufacturers would be freed from the need to specify the mask writer to be used when ordering masks to mask manufacturers.

  8. Magnetic Fields and Galactic Star Formation Rates (United States)

    Van Loo, Sven; Tan, Jonathan C.; Falle, Sam A. E. G.


    The regulation of galactic-scale star formation rates (SFRs) is a basic problem for theories of galaxy formation and evolution: which processes are responsible for making observed star formation rates so inefficient compared to maximal rates of gas content divided by dynamical timescale? Here we study the effect of magnetic fields of different strengths on the evolution of giant molecular clouds (GMCs) within a kiloparsec patch of a disk galaxy and resolving scales down to ≃ 0.5 pc. Including an empirically motivated prescription for star formation from dense gas ({{n}H}\\gt {{10}5} c{{m}-3}) at an efficiency of 2% per local free-fall time, we derive the amount of suppression of star formation by magnetic fields compared to the nonmagnetized case. We find GMC fragmentation, dense clump formation, and SFR can be significantly affected by the inclusion of magnetic fields, especially in our strongest investigated B-field case of 80 μG. However, our chosen kiloparsec-scale region, extracted from a global galaxy simulation, happens to contain a starbursting cloud complex that is only modestly affected by these magnetic fields and likely requires internal star formation feedback to regulate its SFR.

  9. An Algorithm for Autonomous Formation Obstacle Avoidance (United States)

    Cruz, Yunior I.

    The level of human interaction with Unmanned Aerial Systems varies greatly from remotely piloted aircraft to fully autonomous systems. In the latter end of the spectrum, the challenge lies in designing effective algorithms to dictate the behavior of the autonomous agents. A swarm of autonomous Unmanned Aerial Vehicles requires collision avoidance and formation flight algorithms to negotiate environmental challenges it may encounter during the execution of its mission, which may include obstacles and chokepoints. In this work, a simple algorithm is developed to allow a formation of autonomous vehicles to perform point to point navigation while avoiding obstacles and navigating through chokepoints. Emphasis is placed on maintaining formation structures. Rather than breaking formation and individually navigating around the obstacle or through the chokepoint, vehicles are required to assemble into appropriately sized/shaped sub-formations, bifurcate around the obstacle or negotiate the chokepoint, and reassemble into the original formation at the far side of the obstruction. The algorithm receives vehicle and environmental properties as inputs and outputs trajectories for each vehicle from start to the desired ending location. Simulation results show that the algorithm safely routes all vehicles past the obstruction while adhering to the aforementioned requirements. The formation adapts and successfully negotiates the obstacles and chokepoints in its path while maintaining proper vehicle separation.

  10. Planetesimal formation starts at the snow line (United States)

    Drążkowska, J.; Alibert, Y.


    Context. The formation stage of planetesimals represents a major gap in our understanding of the planet formation process. Late-stage planet accretion models typically make arbitrary assumptions about planetesimal and pebble distribution, while dust evolution models predict that planetesimal formation is only possible at some orbital distances. Aims: We wish to test the importance of the water snow line in triggering the formation of the first planetesimals during the gas-rich phase of a protoplanetary disk, when cores of giant planets have to form. Methods: We connected prescriptions for gas disk evolution, dust growth and fragmentation, water ice evaporation and recondensation, the transport of both solids and water vapor, and planetesimal formation via streaming instability into a single one-dimensional model for protoplanetary disk evolution. Results: We find that processes taking place around the snow line facilitate planetesimal formation in two ways. First, because the sticking properties between wet and dry aggregates change, a "traffic jam" inside of the snow line slows the fall of solids onto the star. Second, ice evaporation and outward diffusion of water followed by its recondensation increases the abundance of icy pebbles that trigger planetesimal formation via streaming instability just outside of the snow line. Conclusions: Planetesimal formation is hindered by growth barriers and radial drift and thus requires particular conditions to take place. The snow line is a favorable location where planetesimal formation is possible for a wide range of conditions, but not in every protoplanetary disk model, however. This process is particularly promoted in large cool disks with low intrinsic turbulence and an increased initial dust-to-gas ratio. The movie attached to Fig. 3 is only available at

  11. Pyruvate formate-lyase interacts directly with the formate channel FocA to regulate formate translocation. (United States)

    Doberenz, Claudia; Zorn, Michael; Falke, Dörte; Nannemann, David; Hunger, Doreen; Beyer, Lydia; Ihling, Christian H; Meiler, Jens; Sinz, Andrea; Sawers, R Gary


    The FNT (formate-nitrite transporters) form a superfamily of pentameric membrane channels that translocate monovalent anions across biological membranes. FocA (formate channel A) translocates formate bidirectionally but the mechanism underlying how translocation of formate is controlled and what governs substrate specificity remains unclear. Here we demonstrate that the normally soluble dimeric enzyme pyruvate formate-lyase (PflB), which is responsible for intracellular formate generation in enterobacteria and other microbes, interacts specifically with FocA. Association of PflB with the cytoplasmic membrane was shown to be FocA dependent and purified, Strep-tagged FocA specifically retrieved PflB from Escherichia coli crude extracts. Using a bacterial two-hybrid system, it could be shown that the N-terminus of FocA and the central domain of PflB were involved in the interaction. This finding was confirmed by chemical cross-linking experiments. Using constraints imposed by the amino acid residues identified in the cross-linking study, we provide for the first time a model for the FocA-PflB complex. The model suggests that the N-terminus of FocA is important for interaction with PflB. An in vivo assay developed to monitor changes in formate levels in the cytoplasm revealed the importance of the interaction with PflB for optimal translocation of formate by FocA. This system represents a paradigm for the control of activity of FNT channel proteins. Copyright © 2014 Elsevier Ltd. All rights reserved.

  12. Leader-Follower Formation Control for Quadrotors (United States)

    Wu, Falin; Chen, Jiemin; Liang, Yuan


    Quadrotors are gaining an increasing interest in public and extensively explored in recent years. In many situations, a team of quadrotors is desired to operate in a certain shape, which is also called formation. In this paper, a linear PID controller is used to control each single quadrotor and a slide mode controller is adopted to solve the formation flying problem which employs the leader-follower structure. The formation simulations are run in the Matlab/Simulink environment to evaluate the performance of control laws.

  13. Star-Formation Histories of MUSCEL Galaxies (United States)

    Young, Jason; Kuzio de Naray, Rachel; Xuesong Wang, Sharon


    The MUSCEL program (MUltiwavelength observations of the Structure, Chemistry and Evolution of LSB galaxies) uses combined ground-based/space-based data to determine the spatially resolved star-formation histories of low surface brightness (LSB) galaxies. LSB galaxies are paradoxical in that they are gas rich but have low star-formation rates. Here we present our observations and fitting technique, and the derived histories for select MUSCEL galaxies. It is our aim to use these histories in tandem with velocity fields and metallicity profiles to determine the physical mechanism(s) that give these faint galaxies low star-formation rates despite ample gas supplies.

  14. L’abandon en formation à distance


    Dussarps, Clément


    Cette contribution questionne l’abandon des apprenants en formation ouverte à distance (FOAD) au prisme de la dimension socioaffective, c’est-à-dire de l’ensemble des sentiments et émotions véhiculés dans et par les relations sociales entre les acteurs du dispositif de formation (enseignants, autres apprenants, proches), et de la dimension motivationnelle. Il s’agira de comprendre comment, en dépit de l’éventuelle solitude que peut induire l’isolement en formation à distance, les apprenants p...

  15. Extrasolar planets formation, detection and dynamics

    CERN Document Server

    Dvorak, Rudolf


    This latest, up-to-date resource for research on extrasolar planets covers formation, dynamics, atmospheres and detection. After a look at the formation of giant planets, the book goes on to discuss the formation and dynamics of planets in resonances, planets in double stars, atmospheres and habitable zones, detection via spectra and transits, and the history and prospects of ESPs as well as satellite projects.Edited by a renowned expert in solar system dynamics with chapters written by the leading experts in the method described -- from the US and Europe -- this is an ideal textbook for g

  16. Biofilm formation in a hot water system

    DEFF Research Database (Denmark)

    Bagh, L.K.; Albrechtsen, Hans-Jørgen; Arvin, Erik


    The biofilm formation rate was measured in situ in a hot water system in an apartment building by specially designed sampling equipment, and the net growth of the suspended bacteria was measured by incubation of water samples with the indigeneous bacteria. The biofilm formation rate reached......, in the sludge, or in the water from the distribution system was negligible. This indicated that bacterial growth took place on the inner surfaces in the hot water system and biofilm formation and detachment of bacteria could account for most of the suspended bacteria actually measured in hot water. Therefore...

  17. Spur Reaction Model of Positronium Formation

    DEFF Research Database (Denmark)

    Mogensen, O. E.


    A new model of positronium (Ps) formation is proposed. Positronium is assumed to be formed by a reaction between a positron and an electron in the positron spur. Ps formation must compete with electron‐ion recombination and electron or positron reactions with solvent molecules and scavenger...... impurities. It is also influenced by electron and positron solvation. The model correlates the measured Ps formation probabilities with the spur electron properties determined in radiation chemistry. The predictions of the model are shown to be in good agreement with experimental results for liquids...

  18. Time-Dependent Dust Formation in Novae

    Directory of Open Access Journals (Sweden)

    Kyung-Won Suh


    Full Text Available The dust formation processes in novae are investigated with close attention to recent infrared observations. Using mainly the classical nucleation theory, we have calculated the time scales of dust formation and growth in the environments of novae. Those time scales roughly resemble the typical observations. We have classified the dust-forming novae into three classes according to their explosion properties and the thermodynamic properties of dust grains. Oxygen grains from much later than carbon grains because of their thermodynamic properties. The effect of grain formation to the efficiency of stellar winds to drive the material outward is tested with newly obtained Planck mean values of dust grains.

  19. Finite time coordinated formation control for spacecraft formation flying under directed communication topology (United States)

    Ran, Dechao; Chen, Xiaoqian; Misra, Arun K.


    This paper investigates the finite time coordinated formation control problem for spacecraft formation flying (SFF) under the assumption of directed communication topology. By using the neighborhood state measurements, a robust finite time coordinated formation controller is firstly designed based on the nonsingular terminal sliding mode surface. To address the special case that the desired trajectory of the formation is only accessible to a subset of spacecraft in the formation, an adaptive finite time coordinated formation controller is also proposed by designing a novel sliding mode surface. In both cases, the external disturbances are explicitly taken into account. Rigorous theoretical analysis proves that the proposed control schemes ensure that the closed-loop system can track the desired time-varying trajectory in finite time. Numerical simulations are presented that not only highlights the closed-loop performance benefits from the proposed control algorithms, but also illustrates the effectiveness in the presence of external disturbances when compared with the existing coordinated formation control schemes.

  20. Defining File Format Obsolescence: A Risky Journey

    Directory of Open Access Journals (Sweden)

    David Pearson


    Full Text Available File format obsolescence is a major risk factor threatening the ongoing usefulness of digital information collections. While the preservation community has become increasingly interested in tools for assessing a wide range of risks, the National Library of Australia is developing mechanisms specifically focused on the risks of format obsolescence. The paper reports on the AONS II Project, undertaken in conjunction with the Australian Partnership for Sustainable Repositories (APSR. The project aimed to refine and develop a software tool that would automatically find and report indicators of obsolescence risks, to help repository managers decide if preservation action is needed. The paper discusses the current mismatch between this objective and the available sources of information on file formats, and emphasises the need to take account of both local and global factors in assessing risk. The paper calls for the preservation community to engage with the further development of thinking about file format obsolescence.

  1. Volume Hologram Formation in SU-8 Photoresist

    Directory of Open Access Journals (Sweden)

    Tina Sabel


    Full Text Available In order to further understand the mechanism of volume hologram formation in photosensitive polymers, light-induced material response is analyzed in commonly used epoxy-based negative photoresist Epon SU-8. For this purpose, time-resolved investigation of volume holographic grating growth is performed in the SU-8 based host–guest system and in the pure SU-8 material, respectively. The comparison of grating growth curves from doped and undoped system allows us to draw conclusions on the impact of individual components on the grating formation process. The successive formation of transient absorption as well as phase gratings in SU-8 is observed. Influence of exposure duration and UV flood cure on the grating growth are investigated. Observed volume holographic grating formation in SU-8 can be explained based on the generation and subsequent diffusion of photoacid as well as time-delayed polymerization of exposed and unexposed areas.

  2. Light field analysis for modeling image formation. (United States)

    Liang, Chia-Kai; Shih, Yi-Chang; Chen, Homer H


    Image formation is traditionally described by a number of individual models, one for each specific effect in the image formation process. However, it is difficult to aggregate the effects by concatenating such individual models. In this paper, we apply light transport analysis to derive a unified image formation model that represents the radiance along a light ray as a 4-D light field signal and physical phenomena such as lens refraction and blocking as linear transformations or modulations of the light field. This unified mathematical framework allows the entire image formation process to be elegantly described by a single equation. It also allows most geometric and photometric effects of imaging, including perspective transformation, defocus blur, and vignetting, to be represented in both 4-D primal and dual domains. The result matches that of traditional models. Generalizations and applications of this theoretic framework are discussed.

  3. MP3 the meaning of a format

    CERN Document Server

    Sterne, Jonathan


    Jonathan Sterne shows that understanding the historical meaning of the MP3, the world's most common format for recorded audio, involves rethinking the place of digital technologies in the broader universe of twentieth-century communication history.

  4. Inflow of atomic gas fuelling star formation

    DEFF Research Database (Denmark)

    Michałowski, M. J.; Gentile, G.; Hjorth, Jeppe


    Gamma-ray burst host galaxies are deficient in molecular gas, and show anomalous metal-poor regions close to GRB positions. Using recent Australia Telescope Compact Array (ATCA) Hi observations we show that they have substantial atomic gas reservoirs. This suggests that star formation in these ga......Gamma-ray burst host galaxies are deficient in molecular gas, and show anomalous metal-poor regions close to GRB positions. Using recent Australia Telescope Compact Array (ATCA) Hi observations we show that they have substantial atomic gas reservoirs. This suggests that star formation...... in these galaxies may be fuelled by recent inflow of metal-poor atomic gas. While this process is debated, it can happen in low-metallicity gas near the onset of star formation because gas cooling (necessary for star formation) is faster than the Hi-to-H2 conversion....

  5. Fracture Fluid Additive and Formation Degradations (United States)

    This presentation is on reactions that describe the degradation of fracturing fluids & formations during the hydraulic fracturing process & the clean‐up period. It contains a description of primary chemical reaction controls, & common degradation reactions

  6. Fabrication of carbohydrate microarrays through boronate formation. (United States)

    Hsiao, Hsuan-Yi; Chen, Mu-Lin; Wu, Huan-Ting; Huang, Li-De; Chien, Wei-Ting; Yu, Ching-Ching; Jan, Fan-Dan; Sahabuddin, Sk; Chang, Tsung-Che; Lin, Chun-Cheng


    A straightforward method for fabricating a stable and covalent carbohydrate microarray based on boronate formation between the hydroxyl groups of carbohydrate and boronic acid (BA) on the glass surface was used to identify carbohydrate-protein interactions.

  7. The Role of Emotions in Delusion Formation

    Directory of Open Access Journals (Sweden)

    Smurzyńska Adrianna


    Full Text Available The text concerns the role of emotions in delusion formation. Provided are definitions from DSM-V and DSM-IV-R and the problems found in those definitions. One of them, the problem of delusion formation, is described when providing cognitive theories of delusions. The core of the paper is a presentation of the emotional and affective disorders in delusions, especially Capgras delusion and Cotard delusion. The author provides a comparison of the kinds of delusions and the conclusions taken from neuroimaging studies. As a result of the fact that an explanation of delusion formation focusing on emotional problems turns out to be insufficient, the author provides examples of the reasoning impairments which coexist with them. At the end of the article, some hypotheses are proposed concerning the role of emotions and reasoning in delusion formation and the relation between belief disorders and emotional disorders.

  8. Microbiological processes in banded iron formation deposition

    DEFF Research Database (Denmark)

    Posth, Nicole R.; Konhauser, Kurt O.; Kappler, Andreas


    Banded iron formations have been studied for decades, particularly regarding their potential as archives of the Precambrian environment. In spite of this effort, the mechanism of their deposition and, specifically, the role that microbes played in the precipitation of banded iron formation minerals......, remains unresolved. Evidence of an anoxic Earth with only localized oxic areas until the Great Oxidation Event ca 2·45 to 2·32 Ga makes the investigation of O2-independent mechanisms for banded iron formation deposition relevant. Recent studies have explored the long-standing proposition that Archean...... banded iron formations may have been formed, and diagenetically modified, by anaerobic microbial metabolisms. These efforts encompass a wide array of approaches including isotope, ecophysiological and phylogeny studies, molecular and mineral marker analysis, and sedimentological reconstructions. Herein...

  9. Simulating Precambrian banded iron formation diagenesis

    DEFF Research Database (Denmark)

    Posth, Nicole R.; K??hler, Inga; D. Swanner, Elizabeth


    Post-depositional diagenetic alteration makes the accurate interpretation of key precipitation processes in ancient sediments, such as Precambrian banded iron formations (BIFs), difficult. While microorganisms are proposed as key contributors to BIF deposition, the diagenetic transformation...

  10. Shedding light on disulfide bond formation

    DEFF Research Database (Denmark)

    Ostergaard, H; Henriksen, A; Hansen, F G


    To visualize the formation of disulfide bonds in living cells, a pair of redox-active cysteines was introduced into the yellow fluorescent variant of green fluorescent protein. Formation of a disulfide bond between the two cysteines was fully reversible and resulted in a >2-fold decrease in the i......To visualize the formation of disulfide bonds in living cells, a pair of redox-active cysteines was introduced into the yellow fluorescent variant of green fluorescent protein. Formation of a disulfide bond between the two cysteines was fully reversible and resulted in a >2-fold decrease...... reorganization of residues in the immediate chromophore environment. By combining this information with spectroscopic data, we propose a detailed mechanism accounting for the observed redox state-dependent fluorescence. The redox potential of the cysteine couple was found to be within the physiological range...

  11. The formation of planets by disc fragmentation

    Directory of Open Access Journals (Sweden)

    Stamatellos Dimitris


    Full Text Available I discuss the role that disc fragmentation plays in the formation of gas giant and terrestrial planets, and how this relates to the formation of brown dwarfs and low-mass stars, and ultimately to the process of star formation. Protostellar discs may fragment, if they are massive enough and can cool fast enough, but most of the objects that form by fragmentation are brown dwarfs. It may be possible that planets also form, if the mass growth of a proto-fragment is stopped (e.g. if this fragment is ejected from the disc, or suppressed and even reversed (e.g by tidal stripping. I will discuss if it is possible to distinguish whether a planet has formed by disc fragmentation or core accretion, and mention of a few examples of observed exoplanets that are suggestive of formation by disc fragmentation.

  12. The NeXus data format

    Energy Technology Data Exchange (ETDEWEB)

    Könnecke, Mark; Akeroyd, Frederick A.; Bernstein, Herbert J.; Brewster, Aaron S.; Campbell, Stuart I.; Clausen, Björn; Cottrell, Stephen; Hoffmann, Jens Uwe; Jemian, Pete R.; Männicke, David; Osborn, Raymond; Peterson, Peter F.; Richter, Tobias; Suzuki, Jiro; Watts, Benjamin; Wintersberger, Eugen; Wuttke, Joachim


    NeXus is an effort by an international group of scientists to define a common data exchange and archival format for neutron, X-ray and muon experiments. NeXus is built on top of the scientific data format HDF5 and adds domain-specific rules for organizing data within HDF5 files, in addition to a dictionary of well defined domain-specific field names. The NeXus data format has two purposes. First, it defines a format that can serve as a container for all relevant data associated with a beamline. This is a very important use case. Second, it defines standards in the form of application definitions for the exchange of data between applications. NeXus provides structures for raw experimental data as well as for processed data.

  13. Transient Exciplex Formation Electron Transfer Mechanism

    Directory of Open Access Journals (Sweden)

    Michael G. Kuzmin


    Full Text Available Transient exciplex formation mechanism of excited-state electron transfer reactions is analyzed in terms of experimental data on thermodynamics and kinetics of exciplex formation and decay. Experimental profiles of free energy, enthalpy, and entropy for transient exciplex formation and decay are considered for several electron transfer reactions in various solvents. Strong electronic coupling in contact pairs of reactants causes substantial decrease of activation energy relative to that for conventional long-range ET mechanism, especially for endergonic reactions, and provides the possibility for medium reorganization concatenated to gradual charge shift in contrast to conventional preliminary medium and reactants reorganization. Experimental criteria for transient exciplex formation (concatenated mechanism of excited-state electron transfer are considered. Available experimental data show that this mechanism dominates for endergonic ET reactions and provides a natural explanation for a lot of known paradoxes of ET reactions.

  14. Complications and mortality following stoma formation. (United States)

    Harris, D. A.; Egbeare, D.; Jones, S.; Benjamin, H.; Woodward, A.; Foster, M. E.


    INTRODUCTION: As stoma formation is thought to be declining, we performed a study to evaluate the rate of stoma formation and the impact on stoma complication rates, together with risk factors for complications. PATIENTS AND METHODS: Stoma incidence, individual complications and mortality rates were retrieved from a stoma nurse database of 345 stomas created over an 8-year period. RESULTS: Stoma formation increased over the study period, although the incidence of complications declined. Stoma complications were more frequently seen in emergency surgery. A significant association between stoma complications and mortality was identified. CONCLUSIONS: Age of patient, urgency of surgery and diagnosis were associated with high levels of morbidity and mortality. Stomas are often formed in frail patients unsuitable for anastomosis formation, which may explain the high mortality in ostomy patients. PMID:16263009

  15. Promoting proximal formative assessment with relational discourse (United States)

    Scherr, Rachel E.; Close, Hunter G.; McKagan, Sarah B.


    The practice of proximal formative assessment - the continual, responsive attention to students' developing understanding as it is expressed in real time - depends on students' sharing their ideas with instructors and on teachers' attending to them. Rogerian psychology presents an account of the conditions under which proximal formative assessment may be promoted or inhibited: (1) Normal classroom conditions, characterized by evaluation and attention to learning targets, may present threats to students' sense of their own competence and value, causing them to conceal their ideas and reducing the potential for proximal formative assessment. (2) In contrast, discourse patterns characterized by positive anticipation and attention to learner ideas increase the potential for proximal formative assessment and promote self-directed learning. We present an analysis methodology based on these principles and demonstrate its utility for understanding episodes of university physics instruction.

  16. Dissipational galaxy formation - Confrontation with observations (United States)

    Silk, J.; Norman, C.


    An exploration is presented of the hypothesis that a protogalaxy consists of an aggregate of interacting gas clouds which undergo mergers with neighboring systems, as envisaged by both the hierarchical clustering and fragmentation schemes of galaxy formation. Both gaseous dissipation and violet relaxation play fundamental roles in this galaxy formation model, in order to account for such diverse structural and dynamical properties of spheroidal galaxies as velocity anisotropy and metallicity gradients. Protogalaxy mergers during the initial stages of galaxy clustering can account for the observed spatial distribution of spiral, S0, and elliptical galaxies, and galaxy formation can occur slowly and at late epochs, since the time scale for disk formation is less than about 10 to the 10th years.

  17. Physics of star formation in galaxies

    CERN Document Server

    Palla, F


    Begining with a historical introduction, ""Star Formation: The Early History"", this text then presents two long articles on ""Pre-Main-Sequence Evolution of Stars and Young Clusters"" and ""Observations of Young Stellar Objects"".

  18. Stochastic Models of Molecule Formation on Dust (United States)

    Charnley, Steven; Wirstroem, Eva


    We will present new theoretical models for the formation of molecules on dust. The growth of ice mantles and their layered structure is accounted for and compared directly to observations through simulation of the expected ice absorption spectra

  19. 4 dnja v drugom formate / Dmitri Babitshenko

    Index Scriptorium Estoniae

    Babitshenko, Dmitri


    25.-28. okt. Teises Teatris toimuvast festivalist "Teine formaat 2. Sügishooaeg" (Drugoi format 2), mille programmis on muusikat, teatrietendusi, luulet, tantsulavastusi ja filme. Festivali organiseerija on Teise Teatri administraator Anastassia Kulkova

  20. Star Formation in low mass galaxies (United States)

    Mehta, Vihang


    Our current hierarchical view of the universe asserts that the large galaxies we see today grew via mergers of numerous smaller galaxies. As evidenced by recent literature, the collective impact of these low mass galaxies on the universe is more substantial than previously thought. Studying the growth and evolution of these low mass galaxies is critical to our understanding of the universe as a whole. Star formation is one of the most important ongoing processes in galaxies. Forming stars is fundamental to the growth of a galaxy. One of the main goals of my thesis is to analyze the star formation in these low mass galaxies at different redshifts.Using the Hubble UltraViolet Ultra Deep Field (UVUDF), I investigate the star formation in galaxies at the peak of the cosmic star formation history using the ultraviolet (UV) light as a star formation indicator. Particularly, I measure the UV luminosity function (LF) to probe the volume-averaged star formation properties of galaxies at these redshifts. The depth of the UVUDF is ideal for a direct measurement of the faint end slope of the UV LF. This redshift range also provides a unique opportunity to directly compare UV to the "gold standard" of star formation indicators, namely the Hα nebular emission line. A joint analysis of the UV and Hα LFs suggests that, on average, the star formation histories in low mass galaxies (~109 M⊙) are more bursty compared to their higher mass counterparts at these redshifts.Complementary to the analysis of the average star formation properties of the bulk galaxy population, I investigate the details of star formation in some very bursty galaxies at lower redshifts selected from Spitzer Large Area Survey with Hyper-Suprime Cam (SPLASH). Using a broadband color-excess selection technique, I identify a sample of low redshift galaxies with bright nebular emission lines in the Subaru-XMM Deep Field (SXDF) from the SPLASH-SXDF catalog. These galaxies are highly star forming and have

  1. Defining File Format Obsolescence: A Risky Journey


    David Pearson; Colin Webb


    File format obsolescence is a major risk factor threatening the ongoing usefulness of digital information collections. While the preservation community has become increasingly interested in tools for assessing a wide range of risks, the National Library of Australia is developing mechanisms specifically focused on the risks of format obsolescence. The paper reports on the AONS II Project, undertaken in conjunction with the Australian Partnership for Sustainable Repositories (APSR). The projec...

  2. Core stability in hedonic coalition formation


    Woeginger, Gerhard J.


    In many economic, social and political situations individuals carry out activities in groups (coalitions) rather than alone and on their own. Examples range from households and sport clubs to research networks, political parties and trade unions. The underlying game theoretic framework is known as coalition formation. This survey discusses the notion of core stability in hedonic coalition formation (where each player's happiness only depends on the other members of his coalition but not on ho...

  3. Signaling Pathways Critical for Tooth Root Formation. (United States)

    Wang, J; Feng, J Q


    Tooth is made of an enamel-covered crown and a cementum-covered root. Studies on crown dentin formation have been a major focus in tooth development for several decades. Interestingly, the population prevalence for genetic short root anomaly (SRA) with no apparent defects in crown is close to 1.3%. Furthermore, people with SRA itself are predisposed to root resorption during orthodontic treatment. The discovery of the unique role of Nfic (nuclear factor I C; a transcriptional factor) in controlling root but not crown dentin formation points to a new concept: tooth crown and root have different control mechanisms. Further genetic mechanism studies have identified more key molecules (including Osterix, β-catenin, and sonic hedgehog) that play a critical role in root formation. Extensive studies have also revealed the critical role of Hertwig's epithelial root sheath in tooth root formation. In addition, Wnt10a has recently been found to be linked to multirooted tooth furcation formation. These exciting findings not only fill the critical gaps in our understanding about tooth root formation but will aid future research regarding the identifying factors controlling tooth root size and the generation of a whole "bio-tooth" for therapeutic purposes. This review starts with human SRA and mainly focuses on recent progress on the roles of NFIC-dependent and NFIC-independent signaling pathways in tooth root formation. Finally, this review includes a list of the various Cre transgenic mouse lines used to achieve tooth root formation-related gene deletion or overexpression, as well as strengths and limitations of each line.

  4. Sequence Determinants of Bacterial Amyloid Formation


    Wang, Xuan; Chapman, Matthew R.


    Amyloids are proteinaceous fibers commonly associated with neurodegenerative diseases and prion-based encephalopathies. Many different polypeptides can form amyloid fibers, leading to the suggestion that amyloid is a primitive main-chain-dominated structure. A growing body of evidence suggests that amino acid side chains dramatically influence amyloid formation. The specific role fulfilled by side chains in amyloid formation, especially in vivo, remains poorly understood. Here, we determined ...

  5. Formation à l’évaluation


    Fossati, Anne


    Cette étude de cas présente, pour une discipline précise, les sciences de la vie et de la terre, une démarche de formation à l’évaluation proposée à des professeurs de collège, dans le cadre d’un stage annuel de la MAFPEN (Mission académique de formation des personnels de l’Éducation nationale) de Versailles.

  6. Pembentukan Resiliensi (Resilient Formation) Pada Penderita Thalassemia


    Kurniawan, Yudi


    Thalassemia is a genetic disorder within hemoglobin formation that cause its patients were given blood transfusion along their life, it make thalassemia patients having difficulties for doing activity and self-actualization. Thalassemia patient in Indonesia increase rapidly within ten years lately (TEMPO magazine January 17th 2010 edition). This research have been used qualitative method with case study design, it is aimed for exploring resilient formation and resilient determinant within th...

  7. Clustered Massive Star Formation in Molecular Clouds


    Tan, Jonathan C.


    I review some important questions in the field of massive star formation: What are the initial conditions for proto star clusters and how do they arise? What are the initial conditions for individual massive star formation within star clusters? How do massive protostars accumulate their mass? I compare the Turbulent Core Model (McKee & Tan 2003) to several nearby regions, including Orion KL. Here I also discuss the origin of BN's high proper motion.

  8. Insights from simulations of star formation

    Energy Technology Data Exchange (ETDEWEB)

    Larson, Richard B [Department of Astronomy, Yale University, Box 208101, New Haven, CT 06520-8101 (United States)


    Although the basic physics of star formation is classical, numerical simulations have yielded essential insights into how stars form. They show that star formation is a highly nonuniform runaway process characterized by the emergence of nearly singular peaks in density, followed by the accretional growth of embryo stars that form at these density peaks. Circumstellar discs often form from the gas being accreted by the forming stars, and accretion from these discs may be episodic, driven by gravitational instabilities or by protostellar interactions. Star-forming clouds typically develop filamentary structures, which may, along with the thermal physics, play an important role in the origin of stellar masses because of the sensitivity of filament fragmentation to temperature variations. Simulations of the formation of star clusters show that the most massive stars form by continuing accretion in the dense cluster cores, and this again is a runaway process that couples star formation and cluster formation. Star-forming clouds also tend to develop hierarchical structures, and smaller groups of forming objects tend to merge into progressively larger ones, a generic feature of self-gravitating systems that is common to star formation and galaxy formation. Because of the large range of scales and the complex dynamics involved, analytic models cannot adequately describe many aspects of star formation, and detailed numerical simulations are needed to advance our understanding of the subject. 'The purpose of computing is insight, not numbers.' Richard W Hamming, in Numerical Methods for Scientists and Engineers (1962) 'There are more things in heaven and earth, Horatio, than are dreamt of in your philosophy.' William Shakespeare, in Hamlet, Prince of Denmark (1604) (key issues review)

  9. SMEs need formative infrastructure for business transformation


    Gäre, Klas; Melin, Ulf


    Purpose – The purpose is to study conditions for ICT-use in SMEs, actors and roles involved and concepts constituting a formative infrastructure. Method - The methodological point of departure and approach in this study is qualitative and more than 60 interviews are performed within a geographical region. Findings – Important findings from the present research is a formative ICT infrastructure can be viewed as constituted by identified needs among SMEs, in (1) sensemaking, (2) sensegiving, an...

  10. The formation of the solar system


    Pfalzner, S.; Davies, M B; Gounelle, M.; Johansen, A.; Muenker, C.; Lacerda, P.; Zwart, S. Portegies; Testi, L.; Trieloff, M.; Veras, D.


    The solar system started to form about 4.56 Gyr ago and despite the long intervening time span, there still exist several clues about its formation. The three major sources for this information are meteorites, the present solar system structure and the planet-forming systems around young stars. In this introduction we give an overview of the current understanding of the solar system formation from all these different research fields. This includes the question of the lifetime of the solar pro...

  11. Searching for Star Formation Beyond Reionization


    Barton, Elizabeth J.; Dav'e, Romeel; Smith, John-David T.; Papovich, Casey; Hernquist, Lars; Springel, Volker


    The goal of searching back in cosmic time to find star formation during the epoch of reionization will soon be within reach. We assess the detectability of high-redshift galaxies by combining cosmological hydrodynamic simulations of galaxy formation, stellar evolution models appropriate for the first generations of stars, and estimates of the efficiency for Lyman alpha to escape from forming galaxies into the intergalactic medium. Our simulated observations show that Lyman alpha emission at z...

  12. Star Formation Histories of Nearby Dwarf Galaxies


    Grebel, Eva K.


    Properties of nearby dwarf galaxies are briefly discussed. Dwarf galaxies vary widely in their star formation histories, the ages of their subpopulations, and in their enrichment history. Furthermore, many dwarf galaxies show evidence for spatial variations in their star formation history; often in the form of very extended old populations and radial gradients in age and metallicity. Determining factors in dwarf galaxy evolution appear to be both galaxy mass and environment. We may be observi...

  13. Influence of temperature on methane hydrate formation. (United States)

    Zhang, Peng; Wu, Qingbai; Mu, Cuicui


    During gas hydrate formation process, a phase transition of liquid water exists naturally, implying that temperature has an important influence on hydrate formation. In this study, methane hydrate was formed within the same media. The experimental system was kept at 1.45, 6.49, and 12.91 °C respectively, and then different pressurization modes were applied in steps. We proposed a new indicator, namely the slope of the gas flow rates against time (dν g /dt), to represent the intrinsic driving force for hydrate formation. The driving force was calculated as a fixed value at the different stages of formation, including initial nucleation/growth, secondary nucleation/growth, and decay. The amounts of gas consumed at each stage were also calculated. The results show that the driving force during each stage follows an inverse relation with temperature, whereas the amount of consumed gas is proportional to temperature. This opposite trend indicates that the influences of temperature on the specific formation processes and final amounts of gas contained in hydrate should be considered separately. Our results also suggest that the specific ambient temperature under which hydrate is formed should be taken into consideration, when explaining the formation of different configurations and saturations of gas hydrates in natural reservoirs.

  14. A uniform format for ocular imaging devices. (United States)

    Ishikawa, H; Liebmann, J M; Uji, Y; Ritch, R


    To develop a uniform file format of ocular imaging data, including, but not limited to, ultrasound biomicroscopy, optical coherence tomography, and nerve fiber analyzer, capable of being transmitted via Internet or intranet for collaborative research and telemedicine use. File filters were developed as dynamic link libraries (DLLs). These can read the original raw data format of each ocular imaging device. A data file format was also designed to describe these raw data uniformly in three different types of compression: noncompressed, run length compression, and differential pulse code modulation (DPCM). These three file formats were then tested in the following aspects: file size, speed of reading, and speed of writing. Run length compression failed to compress raw data, while DPCM compressed raw data successfully (< or =35.5%). The speed of reading and writing files was slowest in DPCM. However, the actual time of reading and writing was fast enough (<0.6 s) for daily work regardless of file compression methods. The format designed has robust potential to be the standard file format for transmission of any ocular imaging raw data.

  15. libvaxdata: VAX data format conversion routines (United States)

    Baker, Lawrence M.


    libvaxdata provides a collection of routines for converting numeric data-integer and floating-point-to and from the formats used on a Digital Equipment Corporation1 (DEC) VAX 32-bit minicomputer (Brunner, 1991). Since the VAX numeric data formats are inherited from those used on a DEC PDP-11 16-bit minicomputer, these routines can be used to convert PDP-11 data as well. VAX numeric data formats are also the default data formats used on DEC Alpha 64-bit minicomputers running OpenVMS The libvaxdata routines are callable from Fortran or C. They require that the caller use two's-complement format for integer data and IEEE 754 format (ANSI/IEEE, 1985) for floating-point data. They also require that the 'natural' size of a C int type (integer) is 32 bits. That is the case for most modern 32-bit and 64-bit computer systems. Nevertheless, you may wish to consult the Fortran or C compiler documentation on your system to be sure. Some Fortran compilers support conversion of VAX numeric data on-the-fly when reading or writing unformatted files, either as a compiler option or a run-time I/O option. This feature may be easier to use than the libvaxdata routines. Consult the Fortran compiler documentation on your system to determine if this alternative is available to you. 1Later Compaq Computer Corporation, now Hewlett-Packard Company

  16. Converting Taxonomic Descriptions to New Digital Formats

    Directory of Open Access Journals (Sweden)

    Hong Cui


    Full Text Available Abstract.--The majority of taxonomic descriptions is currently in print format. The majority of digital descriptions are in formats such as DOC, HTML, or PDF and for human readers. These formats do not convey rich semantics in taxonomic descriptions for computer-aided process. Newer digital formats such as XML and RDF accommodate semantic annotations that allow computers to process the rich semantics on human's behalf, thus open up opportunities for a wide range of innovative usages of taxonomic descriptions, such as searching in more precise and flexible ways, integrating with gnomic and geographic information, generating taxonomic keys automatically, and text data mining and information visualization etc. This paper discusses the challenges in automated conversion of multiple collections of descriptions to XML format and reports an automated system, MARTT. MARTT is a machine-learning system that makes use of training examples to tag new descriptions into XML format. A number of utilities are implemented as solutions to the challenges. The utilities are used to reduce the effort for training example preparation, to facilitate the creation of a comprehensive schema, and to predict system performance on a new collection of descriptions. The system has been tested with several plant and alga taxonomic publications including Flora of China and Flora of North America.

  17. How Galactic Environment Regulates Star Formation (United States)

    Meidt, Sharon E.


    In a new simple model I reconcile two contradictory views on the factors that determine the rate at which molecular clouds form stars—internal structure versus external, environmental influences—providing a unified picture for the regulation of star formation in galaxies. In the presence of external pressure, the pressure gradient set up within a self-gravitating turbulent (isothermal) cloud leads to a non-uniform density distribution. Thus the local environment of a cloud influences its internal structure. In the simple equilibrium model, the fraction of gas at high density in the cloud interior is determined simply by the cloud surface density, which is itself inherited from the pressure in the immediate surroundings. This idea is tested using measurements of the properties of local clouds, which are found to show remarkable agreement with the simple equilibrium model. The model also naturally predicts the star formation relation observed on cloud scales and at the same time provides a mapping between this relation and the closer-to-linear molecular star formation relation measured on larger scales in galaxies. The key is that pressure regulates not only the molecular content of the ISM but also the cloud surface density. I provide a straightforward prescription for the pressure regulation of star formation that can be directly implemented in numerical models. Predictions for the dense gas fraction and star formation efficiency measured on large-scales within galaxies are also presented, establishing the basis for a new picture of star formation regulated by galactic environment.

  18. Recognizing team formation in american football

    KAUST Repository

    Atmosukarto, Indriyati


    Most existing software packages for sports video analysis require manual annotation of important events in the video. Despite being the most popular sport in the United States, most American football game analysis is still done manually. Line of scrimmage and offensive team formation recognition are two statistics that must be tagged by American Football coaches when watching and evaluating past play video clips, a process which takesmanyman hours per week. These two statistics are the building blocks for more high-level analysis such as play strategy inference and automatic statistic generation. In this chapter, we propose a novel framework where given an American football play clip, we automatically identify the video frame in which the offensive team lines in formation (formation frame), the line of scrimmage for that play, and the type of player formation the offensive team takes on. The proposed framework achieves 95% accuracy in detecting the formation frame, 98% accuracy in detecting the line of scrimmage, and up to 67%accuracy in classifying the offensive team’s formation. To validate our framework, we compiled a large dataset comprising more than 800 play-clips of standard and high definition resolution from real-world football games. This dataset will be made publicly available for future comparison.

  19. Acoustic vibration can enhance bacterial biofilm formation. (United States)

    Murphy, Mark F; Edwards, Thomas; Hobbs, Glyn; Shepherd, Joanna; Bezombes, Frederic


    This paper explores the use of low-frequency-low-amplitude acoustic vibration on biofilm formation. Biofilm development is thought to be governed by a diverse range of environmental signals and much effort has gone into researching the effects of environmental factors including; nutrient availability, pH and temperature on the growth of biofilms. Many biofilm-forming organisms have evolved to thrive in mechanically challenging environments, for example soil yet, the effects of the physical environment on biofilm formation has been largely ignored. Exposure of Pseudomonas aeruginosa to vibration at 100, 800 and 1600 Hz for 48 h, resulted in a significant increase in biofilm formation compared with the control, with the greatest growth seen at 800 Hz vibration. The results also show that this increase in biofilm formation is accompanied with an increase in P. aeruginosa cell number. Acoustic vibration was also found to regulate the spatial distribution of biofilm formation in a frequency-dependent manner. Exposure of Staphylococcus aureus to acoustic vibration also resulted in enhanced biofilm formation with the greatest level of biofilm being formed following 48 h exposure at 1600 Hz. These results show that acoustic vibration can be used to control biofilm formation and therefore presents a novel and potentially cost effective means to manipulate the development and yield of biofilms in a range of important industrial and medical processes. Copyright © 2016 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.

  20. Free-format RPG IV the express guide to learning free format

    CERN Document Server

    Martin, Jim


    Focusing strictly on teaching free-format RPG programming methods, this book will help programmers wishing to upgrade their skills   This how-to guide offers a concise and thorough introduction to the increased productivity, better readability, and easier program maintenance that comes with the free-format style of programming in RPG IV. Although free-format information is available in IBM manuals, it is not separated from everything else, requiring hours of tedious research to track down the information needed. This book provides everything you need to know to write RPG IV in the free-format

  1. Television Format As a Site of Cultural Negotiation: Studying the Structures, Agencies and Practices of Format Adaptation

    NARCIS (Netherlands)

    Keinonen, Heidi


    abstractDespite the growing number of publications on television formats, specific theorisations regarding formats and format adaptation, in particular, are still rare. In this article, I introduce a synthesizing approach for studying format appropriation. Drawing on format study, media industry

  2. Observational constraints on Acrretion disk formation (United States)

    Harsono, Daniel; Jørgensen, Jes; van Dishoeck, Ewine; Hogerheijde, Michiel; Bruderer, Simon; Persson, Magnus; Mottram, Joseph


    Stable rotationally supported disks (RSDs) are important for the star and planet formation process. The structure and stability of the RSDs are linked to the accretion process onto the star and the evolution of the protostellar system. Additionally, these disks are composed of infalling material that encounter a wide range of physical conditions. The history of these changes affect the chemical structure and evolution of the accretion disk and, thus, the material out of which planets are formed. The formation of RSDs is not well understood and it is unclear from the existing data at which stage the young disks are rotationally supported. Here, we present new PdBI observations of 13CO and C18O toward 4 Class I YSOs with higher spatial resolution and significantly higher sensitivity than previously possible. The high quality data allow us to constrain the physical structure of the young embedded disks which are rotationally supported within the inner 100 AU radius. Furthermore, the extent of the RSD is smaller than that of the dust disk. The observed physical structure of embedded disks are compared to semi-analytical disk formation models which suggests that the formation process is consistent with inside-out formation. ALMA is needed to confirm the extent of the rotationally supported structure.

  3. Star Cluster Structure from Hierarchical Star Formation (United States)

    Grudic, Michael; Hopkins, Philip; Murray, Norman; Lamberts, Astrid; Guszejnov, David; Schmitz, Denise; Boylan-Kolchin, Michael


    Young massive star clusters (YMCs) spanning 104-108 M⊙ in mass generally have similar radial surface density profiles, with an outer power-law index typically between -2 and -3. This similarity suggests that they are shaped by scale-free physics at formation. Recent multi-physics MHD simulations of YMC formation have also produced populations of YMCs with this type of surface density profile, allowing us to narrow down the physics necessary to form a YMC with properties as observed. We show that the shallow density profiles of YMCs are a natural result of phase-space mixing that occurs as they assemble from the clumpy, hierarchically-clustered configuration imprinted by the star formation process. We develop physical intuition for this process via analytic arguments and collisionless N-body experiments, elucidating the connection between star formation physics and star cluster structure. This has implications for the early-time structure and evolution of proto-globular clusters, and prospects for simulating their formation in the FIRE cosmological zoom-in simulations.

  4. Multimodal pore formation in calcium phosphate cements. (United States)

    Lodoso-Torrecilla, Irene; van Gestel, Nicole A P; Diaz-Gomez, Luis; Grosfeld, Eline-Claire; Laperre, Kjell; Wolke, Joop G C; Smith, Brandon T; Arts, Jacobus J; Mikos, Antonios G; Jansen, John A; Hofmann, Sandra; van den Beucken, Jeroen J J P


    Calcium phosphate cements (CPCs) are commonly used as bone substitute materials. However, their slow degradation rate and lack of macroporosity hinders new bone formation. Poly(dl-lactic-co-glycolic acid) (PLGA) incorporation is of great interest as, upon degradation, produces acidic by-products that enhance CPC degradation. Yet, new bone formation is delayed until PLGA degradation occurs a few weeks after implantation. Therefore, the aim of this study was to accelerate the early stage pore formation within CPCs in vitro. With that purpose, we incorporated the water-soluble porogen sucrose at different weight percentages (10 or 20 wt %) to CPC and CPC/PLGA composites. The results revealed that incorporation of sucrose porogens increased mass loss within the first week of in vitro degradation in groups containing sucrose compared to control groups. After week 1, a further mass loss was observed related to PLGA and CPC degradation. Macroporosity analysis confirmed that macroporosity formation is influenced by the dissolution of sucrose at an early stage and by the degradation of PLGA and CPC at a later stage. We concluded that the combination of sucrose and PLGA porogens in CPC is a promising approach to promote early stage bone tissue ingrowth and complete replacement of CPC through multimodal pore formation. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2017. © 2017 Wiley Periodicals, Inc.

  5. Substructures in Simulations of Relativistic Jet Formation (United States)

    Garcia, Raphael de Oliveira; Oliveira, Samuel Rocha de


    We present a set of simulations of relativistic jets from accretion disk initial setup with numerical solutions of a system of general-relativistic magnetohydrodynamics (GRMHD) partial differential equations in a fixed black hole (BH) spacetime which is able to show substructures formations inside the jet as well as lobe formation on the jet head. For this, we used a central scheme of finite volume method without dimensional split and with no Riemann solvers namely the Nessyahu-Tadmor method. Thus, we were able to obtain stable numerical solutions with spurious oscillations under control and with no excessive numerical dissipation. Therefore, we developed some setups for initial conditions capable of simulating the formation of relativistic jets from the accretion disk falling onto central black hole until its ejection, both immersed in a magnetosphere. In our simulations, we were able to observe some substructure of a jet created from an accretion initial disk, namely, jet head, knots, cocoon, and lobe. Also, we present an explanation for cocoon formation and lobe formation. Each initial scenario was determined by ratio between disk density and magnetosphere density, showing that this relation is very important for the shape of the jet and its substructures.

  6. The formation of the solar system (United States)

    Pfalzner, S.; Davies, M. B.; Gounelle, M.; Johansen, A.; Münker, C.; Lacerda, P.; Portegies Zwart, S.; Testi, L.; Trieloff, M.; Veras, D.


    The solar system started to form about 4.56 Gyr ago and despite the long intervening time span, there still exist several clues about its formation. The three major sources for this information are meteorites, the present solar system structure and the planet-forming systems around young stars. In this introduction we give an overview of the current understanding of the solar system formation from all these different research fields. This includes the question of the lifetime of the solar protoplanetary disc, the different stages of planet formation, their duration, and their relative importance. We consider whether meteorite evidence and observations of protoplanetary discs point in the same direction. This will tell us whether our solar system had a typical formation history or an exceptional one. There are also many indications that the solar system formed as part of a star cluster. Here we examine the types of cluster the Sun could have formed in, especially whether its stellar density was at any stage high enough to influence the properties of today’s solar system. The likelihood of identifying siblings of the Sun is discussed. Finally, the possible dynamical evolution of the solar system since its formation and its future are considered.

  7. Crenarchaeal biofilm formation under extreme conditions.

    Directory of Open Access Journals (Sweden)

    Andrea Koerdt

    Full Text Available BACKGROUND: Biofilm formation has been studied in much detail for a variety of bacterial species, as it plays a major role in the pathogenicity of bacteria. However, only limited information is available for the development of archaeal communities that are frequently found in many natural environments. METHODOLOGY: We have analyzed biofilm formation in three closely related hyperthermophilic crenarchaeotes: Sulfolobus acidocaldarius, S. solfataricus and S. tokodaii. We established a microtitre plate assay adapted to high temperatures to determine how pH and temperature influence biofilm formation in these organisms. Biofilm analysis by confocal laser scanning microscopy demonstrated that the three strains form very different communities ranging from simple carpet-like structures in S. solfataricus to high density tower-like structures in S. acidocaldarius in static systems. Lectin staining indicated that all three strains produced extracellular polysaccharides containing glucose, galactose, mannose and N-acetylglucosamine once biofilm formation was initiated. While flagella mutants had no phenotype in two days old static biofilms of S. solfataricus, a UV-induced pili deletion mutant showed decreased attachment of cells. CONCLUSION: The study gives first insights into formation and development of crenarchaeal biofilms in extreme environments.

  8. Formation of triploid plants via possible polyspermy. (United States)

    Toda, Erika; Okamoto, Takashi


    Polyploidization is a common phenomenon in angiosperms, and polyploidy has played a major role in the long-term diversification and evolutionary success of plants. Triploid plants are considered as the intermediate stage in the formation of stable autotetraploid plants, and this pathway of tetraploid formation is known as the triploid bridge. As for the mechanism of triploid formation among diploid populations, fusion of an unreduced gamete with a reduced gamete is generally accepted. In addition, the possibility of polyspermy has been proposed for maize, wheat and some orchids, although it has been regarded as an uncommon mechanism of polyploid formation. One of the reasons why polyspermy is regarded as uncommon is because it is difficult to reproduce the polyspermy situation in zygotes and to analyze the developmental profiles of polyspermic zygotes. In the study, we produced polyspermic rice zygotes by electric fusion of an egg cell with two sperm cells and monitored their developmental profiles. The two sperm nuclei and the egg nucleus fused into a zygotic nucleus in the polyspermic zygote, and the triploid zygote divided into a two-celled embryo via mitotic division with a typical bipolar microtubule spindle. The two-celled proembryos developed and regenerated into triploid plants. These results suggest that polyspermic plant zygotes have the potential to form triploid embryos, and that polyspermy in angiosperms might be a pathway for the formation of triploid plants.




    Wegener, Warner S. (University of Cincinnati, Cincinnati, Ohio), and Antonio H. Romano. Control of isocitratase formation in Rhizopus nigricans. J. Bacteriol. 87:156-161. 1964.-A fumaric acid-producing strain of Rhizopus nigricans was found to produce a fair level of isocitratase in a casein hydrolysate medium. Glucose repressed enzyme formation. When glucose was utilized during growth, there was a relief of repression, and enzyme synthesis was resumed at a rate equivalent to that found in nonrepressed cells. Zinc stimulated isocitratase formation in glucose-repressed cultures by stimulating growth and glucose utilization, thereby decreasing accumulation of repressor metabolites derived from glucose. The effectiveness of acetate as an inducer was greater on glucose-repressed cells than on nonrepressed cells; cells grown in the presence of glucose formed higher levels of isocitratase when subsequently replaced with an acetate-containing inductive medium than did cells grown without glucose. Moreover, addition of 2 ppm of Zn(++) during the inductive replacement phase resulted in a twofold increase in isocitratase formation. The hypothesis is submitted that Zn(++) exerts its action by stimulating ribonucleic acid (RNA) synthesis, thereby facilitating the formation of a specific RNA during induction. Preliminary evidence implicating Zn(++) in the stimulation of RNA synthesis in this organism is presented.

  10. Cloud Optimized Image Format and Compression (United States)

    Becker, P.; Plesea, L.; Maurer, T.


    Cloud based image storage and processing requires revaluation of formats and processing methods. For the true value of the massive volumes of earth observation data to be realized, the image data needs to be accessible from the cloud. Traditional file formats such as TIF and NITF were developed in the hay day of the desktop and assumed fast low latency file access. Other formats such as JPEG2000 provide for streaming protocols for pixel data, but still require a server to have file access. These concepts no longer truly hold in cloud based elastic storage and computation environments. This paper will provide details of a newly evolving image storage format (MRF) and compression that is optimized for cloud environments. Although the cost of storage continues to fall for large data volumes, there is still significant value in compression. For imagery data to be used in analysis and exploit the extended dynamic range of the new sensors, lossless or controlled lossy compression is of high value. Compression decreases the data volumes stored and reduces the data transferred, but the reduced data size must be balanced with the CPU required to decompress. The paper also outlines a new compression algorithm (LERC) for imagery and elevation data that optimizes this balance. Advantages of the compression include its simple to implement algorithm that enables it to be efficiently accessed using JavaScript. Combing this new cloud based image storage format and compression will help resolve some of the challenges of big image data on the internet.

  11. Star formation in evolving molecular clouds (United States)

    Völschow, M.; Banerjee, R.; Körtgen, B.


    Molecular clouds are the principle stellar nurseries of our universe; they thus remain a focus of both observational and theoretical studies. From observations, some of the key properties of molecular clouds are well known but many questions regarding their evolution and star formation activity remain open. While numerical simulations feature a large number and complexity of involved physical processes, this plethora of effects may hide the fundamentals that determine the evolution of molecular clouds and enable the formation of stars. Purely analytical models, on the other hand, tend to suffer from rough approximations or a lack of completeness, limiting their predictive power. In this paper, we present a model that incorporates central concepts of astrophysics as well as reliable results from recent simulations of molecular clouds and their evolutionary paths. Based on that, we construct a self-consistent semi-analytical framework that describes the formation, evolution, and star formation activity of molecular clouds, including a number of feedback effects to account for the complex processes inside those objects. The final equation system is solved numerically but at much lower computational expense than, for example, hydrodynamical descriptions of comparable systems. The model presented in this paper agrees well with a broad range of observational results, showing that molecular cloud evolution can be understood as an interplay between accretion, global collapse, star formation, and stellar feedback.

  12. Performance of Different Acids on Sandstone Formations

    Directory of Open Access Journals (Sweden)

    M. A. Zaman


    Full Text Available Stimulation of sandstone formations is a challenging task, which involves several chemicals and physical interactions of the acid with the formation. Some of these reactions may result in formation damage. Mud acid has been successfully used to stimulate sandstone reservoirs for a number of years. It is a mixture of hydrofluoric (HF and hydrochloric (HCl acids designed to dissolve clays and siliceous fines accumulated in the near-wellbore region. Matrix acidizing may also be used to increase formation permeability in undamaged wells. The change may be up to 50% to 100% with the mud acid. For any acidizing process, the selection of acid (Formulation and Concentration and the design (Pre-flush, Main Acid, After-flush is very important. Different researchers are using different combinations of acids with different concentrations to get the best results for acidization. Mainly the common practice is combination of Hydrochloric Acid – Hydrofluoric with Concentration (3% HF – 12% HCl. This paper presents the results of a laboratory investigation of Orthophosphoric acid instead of hydrochloric acid in one combination and the second combination is Fluoboric and formic acid and the third one is formic and hydrofluoric acid. The results are compared with the mud acid and the results calculated are porosity, permeability, and FESEM Analysis and Strength tests. All of these new combinations shows that these have the potential to be used as acidizing acids on sandstone formations.


    Directory of Open Access Journals (Sweden)

    О Ю Заславская


    Full Text Available The article considers modern possibilities of information and communication technologies for the design of electronic educational resources. The conceptual basis of the open educational multimedia system is based on the modular architecture of the electronic educational resource. The content of the electronic training module can be implemented in several versions of the modules: obtaining information, practical exercises, control. The regularities in the teaching process in modern pedagogical theory are considered: general and specific, and the principles for the formation of the content of instruction at different levels are defined, based on the formulated regularities. On the basis of the analysis, the principles of the formation of the electronic educational resource are determined, taking into account the general and didactic patterns of teaching.As principles of the formation of educational material for obtaining information for the electronic educational resource, the article considers: the principle of methodological orientation, the principle of general scientific orientation, the principle of systemic nature, the principle of fundamentalization, the principle of accounting intersubject communications, the principle of minimization. The principles of the formation of the electronic training module of practical studies in the article include: the principle of systematic and dose based consistency, the principle of rational use of study time, the principle of accessibility. The principles of the formation of the module for monitoring the electronic educational resource can be: the principle of the operationalization of goals, the principle of unified identification diagnosis.

  14. Formation of cluster policy in Russian regions

    Directory of Open Access Journals (Sweden)

    Svetlana Nikolaevna Kotlyarova


    Full Text Available This paper deals with the problems of legal regulation of cluster development in the Russian Federation at the regional level. Basic regulations governing the formation and development of clusters were put in a system. It is concluded that own regulatory regions, and regions using mainly general federal approach to the formation of cluster policy are poorly developed. The practice of clustering of the Russian Federation was generalized. Prerequisites for the formation of clusters in the regions and their subsequent development were identified. A comparison of approaches to the formation of cluster policy in the Russian Federation was made, particularities in the areas of the clusters were highlighted and the types of state support for their formation were determined. The basic reasons for the development of regional clusters are: construction of the type of dominant firms, mutual cooperation of cluster members, active support from government and regional authorities (public-private partnerships and personal involvement of management in the region and bottom-up approach.

  15. Analysis of pectic epitopes recognised by hybridoma and phage display monoclonal antibodies using defined oligosaccharides, polysaccharides, and enzymatic degradation

    DEFF Research Database (Denmark)

    Willats, William George Tycho; Limberg, G.; Buchholt, H.C.


    The structure of epitopes recognised by anti-pectin monoclonal antibodies (mAbs) has been investigated using a series of model lime-pectin samples with defined degrees and patterns of methyl esterification, a range of defined oligogalacturonides and enzymatic degradation of pectic polysaccharides...

  16. What Do They Understand? Using Technology to Facilitate Formative Assessment (United States)

    Mitten, Carolyn; Jacobbe, Tim; Jacobbe, Elizabeth


    Formative assessment is so important to inform teachers' planning. A discussion of the benefits of using technology to facilitate formative assessment explains how four primary school teachers adopted three different apps to make their formative assessment more meaningful and useful.

  17. Site-directed, Ligase-Independent Mutagenesis (SLIM): a single-tube methodology approaching 100% efficiency in 4 h. (United States)

    Chiu, Joyce; March, Paul E; Lee, Ryan; Tillett, Daniel


    Site-directed, Ligase-Independent Mutagenesis (SLIM) is a novel PCR-mediated mutagenesis approach that can accommodate all three sequence modification types (insertion, deletion and substitution). The method utilizes an inverse PCR amplification of the template by two tailed long primers and two short primers in a single reaction with all steps carried out in one tube. The tailed primers are designed to contain the desired mutation on complementary overhangs at the terminus of PCR products. Upon post-amplification denaturation and re-annealing, heteroduplex formation between the mixed PCR products creates the desired clonable mutated plasmid. The technique is highly robust and suitable for applications in high-throughput gene engineering and library constructions. In this study, SLIM was employed to create sequence insertions, deletion and substitution within bacteriophage T7 gene 5. The overall efficiency for obtaining the desired product was >95%.


    Directory of Open Access Journals (Sweden)

    Zhilyuk Sergey Aleksandrovich


    Full Text Available The article presents the properties of exogenous word-formation system taking into account the existence of two word-formation systems in modern German. On the basis of foreign research which reveal modern trends in German word-formation connected with the internationalization and the development of new European Latin language. The author defines key features of exogenous word-formation, i.e. foreign origin of wordformation units, unmotivated units, unmotivated interchange in base and affixes as well as limited distribution rules in combination with German word-formation. The article analyzes various approaches to word-division, as well as motivated and unmotivated interchange of consonants in bases and in affixes. Unmotivated interchange showcases a special status of the exogenous word-formation within German. Another item covered by the article is the issue of confix. The article has opinions of researchers about correctness of its separation and a list of its features. The author presents his definition of confix: a confix is a bound exogenous word-formation unit with a certain lexical and semantic meaning and joining other units directly or indirectly (through linking morpheme -o-, which is able to make a base. Moreover, some confixes are able to participate at word-combination and have unlimited distribution. So far, confix showcases the integration of exogenous word-formation and traditional German word-formation. The research proves the special status of exogenous word-formation in German. Its results can be used as a base for further analysis of co-existing word-formation systems in German and determination of their characteristic features.

  19. Whisker Formation On Galvanic Tin Surface Layer

    Directory of Open Access Journals (Sweden)

    Radanyi A.L.


    Full Text Available The present work reports the effect of substrate composition, thickness of the tin electroplate and its morphology on pressure-induced tin whisker formation. Pure tin deposits of different thickness were obtained on a copper and brass substrates using methane sulfonic industrial bath. The deposits were compressed by a steel bearing ball forming imprint on the surface. The microstructure of tin whiskers obtained at the boundary of each imprint, their length and number were studied using both light and scanning electron microscopy. It was shown that the most intensive formation and growth of whiskers was observed in the first two hours. In general, brass substrate was shown to be more prone to whisker formation than copper independently of the tin coating thickness. The results have been compared with industrial bright tin finish on control unit socket leads and proposals have been made as to modification of the production process in order to minimize the risk of whiskering.

  20. Transmission Electron Microscopy Physics of Image Formation

    CERN Document Server

    Kohl, Helmut


    Transmission Electron Microscopy: Physics of Image Formation presents the theory of image and contrast formation, and the analytical modes in transmission electron microscopy. The principles of particle and wave optics of electrons are described. Electron-specimen interactions are discussed for evaluating the theory of scattering and phase contrast. Also discussed are the kinematical and dynamical theories of electron diffraction and their applications for crystal-structure analysis and imaging of lattices and their defects. X-ray microanalysis and electron energy-loss spectroscopy are treated as analytical methods. Specimen damage and contamination by electron irradiation limits the resolution for biological and some inorganic specimens. This fifth edition includes discussion of recent progress, especially in the area of aberration correction and energy filtering; moreover, the topics introduced in the fourth edition have been updated. Transmission Electron Microscopy: Physics of Image Formation is written f...

  1. Linking the Scales of Star Formation (United States)

    Calzetti, Daniela; and the LEGUS Team


    Understanding galaxy evolution requires understanding star formation and its dependence on the local environment, spanning the scales from individual stars to kpc–size structures. The physical conditions within galaxies determine the formation of stars, star clusters, and larger structures, and their subsequent evolution. Observations of external galaxies with the HST that include the UV have enabled the characterization of the young stellar populations with unprecedented accuracy and detail, thus aiding the census and description of those populations. We are now in a position to quantify the spatial distribution and clustering of young stars, and investigate the impact and imprint of the physical conditions of both the local and global environment on the formation and evolution of the multi-scale structures. This talk describes the Legacy ExtraGalactic UV Survey (LEGUS), an HST Treasury programs aimed at investigating these issues using multi-color imaging, from the near-UV to the I, of a sample of fifty nearby galaxies.

  2. Dust Evolution and the Formation of Planetesimals (United States)

    Birnstiel, T.; Fang, M.; Johansen, A.


    The solid content of circumstellar disks is inherited from the interstellar medium: dust particles of at most a micrometer in size. Protoplanetary disks are the environment where these dust grains need to grow at least 13 orders of magnitude in size. Our understanding of this growth process is far from complete, with different physics seemingly posing obstacles to this growth at various stages. Yet, the ubiquity of planets in our galaxy suggests that planet formation is a robust mechanism. This chapter focuses on the earliest stages of planet formation, the growth of small dust grains towards the gravitationally bound "planetesimals", the building blocks of planets. We will introduce some of the key physics involved in the growth processes and discuss how they are expected to shape the global behavior of the solid content of disks. We will consider possible pathways towards the formation of larger bodies and conclude by reviewing some of the recent observational advances in the field.

  3. Protostar formation in the early universe. (United States)

    Yoshida, Naoki; Omukai, Kazuyuki; Hernquist, Lars


    The nature of the first generation of stars in the universe remains largely unknown. Observations imply the existence of massive primordial stars early in the history of the universe, and the standard theory for the growth of cosmic structure predicts that structures grow hierarchically through gravitational instability. We have developed an ab initio computer simulation of the formation of primordial stars that follows the relevant atomic and molecular processes in a primordial gas in an expanding universe. The results show that primeval density fluctuations left over from the Big Bang can drive the formation of a tiny protostar with a mass 1% that of the Sun. The protostar is a seed for the subsequent formation of a massive primordial star.

  4. Pollutant Formation in Monodisperse Fuel Spray Combustion (United States)

    Cernansky, N. P.; Sarv, H.


    The combustion of liquid sprays represents an extremely important class of combustion processes. In the transition region, encompassing droplet sizes in the range of 25-80 micron diameter, the mixing and evaporation processes are both incomplete at the flame front and burning occurs in a combined diffusive and premixed fashion. Under these conditions, the relative importance of heterogeneous and homogeneous effects in dominating the combustion process is switched and gives rise to a number of interesting phenomena. NO (sub x) formation in monodisperse spray combustion was investigated with the following specific objectives: (1) to quantitatively determine the effect of droplet size, number density, etc. on NO sub x formation in monodisperse fuel spray combustion; and (2) to isolate the important physical and chemical phenomena in NO sub x formation in these combustion systems.

  5. In situ oxidation of subsurface formations (United States)

    Beer, Gary Lee [Houston, TX; Mo, Weijian [Sugar Land, TX; Li, Busheng [Houston, TX; Shen, Chonghui [Calgary, CA


    Methods and systems for treating a hydrocarbon containing formation described herein include providing heat to a first portion of the formation from a plurality of heaters in the first portion, producing produced through one or more production wells in a second portion of the formation, reducing or turning off heat provided to the first portion after a selected time, providing an oxidizing fluid through one or more of the heater wells in the first portion, providing heat to the first portion and the second portion through oxidation of at least some hydrocarbons in the first portion, and producing fluids through at least one of the production wells in the second portion. The produced fluids may include at least some oxidized hydrocarbons produced in the first portion.

  6. Formatting Design Dialogues – Games and Participation

    DEFF Research Database (Denmark)

    Brandt, Eva; Messeter, Jörn; Binder, Thomas


    . Next we look more closely at what constitutes game and play, we discuss other authors’ use of games in collaborative settings and finally we examine the board game as a particularly interesting game format. In the second part of the article we present and discuss two board games: the User Game......This article discusses design games as a particular genre for formatting design dialogues. In the first part of the article we review the participatory design literature for game-oriented framings of co-design, and discuss why literal game formats are attractive for facilitating design dialogues...... and the Landscape Game. We show how these games respond to particular challenges, and how they have interesting characteristics in being both ‘as-if’ worlds to explore and shared representations of what the players accomplish. In the last section of the article we discuss how new games may be designed and played...

  7. Catalytic control over supramolecular gel formation (United States)

    Boekhoven, Job; Poolman, Jos M.; Maity, Chandan; Li, Feng; van der Mee, Lars; Minkenberg, Christophe B.; Mendes, Eduardo; van Esch, Jan H.; Eelkema, Rienk


    Low-molecular-weight gels show great potential for application in fields ranging from the petrochemical industry to healthcare and tissue engineering. These supramolecular gels are often metastable materials, which implies that their properties are, at least partially, kinetically controlled. Here we show how the mechanical properties and structure of these materials can be controlled directly by catalytic action. We show how in situ catalysis of the formation of gelator molecules can be used to accelerate the formation of supramolecular hydrogels, which drastically enhances their resulting mechanical properties. Using acid or nucleophilic aniline catalysis, it is possible to make supramolecular hydrogels with tunable gel-strength in a matter of minutes, under ambient conditions, starting from simple soluble building blocks. By changing the rate of formation of the gelator molecules using a catalyst, the overall rate of gelation and the resulting gel morphology are affected, which provides access to metastable gel states with improved mechanical strength and appearance despite an identical gelator composition.

  8. Chondroitin Sulfate Perlecan Enhances Collagen Fibril Formation

    DEFF Research Database (Denmark)

    Kvist, A. J.; Johnson, A. E.; Mörgelin, M.


    in collagen type II fibril assembly by perlecan-null chondrocytes. Cartilage perlecan is a heparin sulfate or a mixed heparan sulfate/chondroitin sulfate proteoglycan. The latter form binds collagen and accelerates fibril formation in vitro, with more defined fibril morphology and increased fibril diameters...... produced in the presence of perlecan. Interestingly, the enhancement of collagen fibril formation is independent on the core protein and is mimicked by chondroitin sulfate E but neither by chondroitin sulfate D nor dextran sulfate. Furthermore, perlecan chondroitin sulfate contains the 4,6-disulfated...... disaccharides typical for chondroitin sulfate E. Indeed, purified glycosaminoglycans from perlecan-enriched fractions of cartilage extracts contain elevated levels of 4,6-disulfated chondroitin sulfate disaccharides and enhance collagen fibril formation. The effect on collagen assembly is proportional...

  9. Understanding coupling between bone resorption and formation

    DEFF Research Database (Denmark)

    Andersen, Thomas Levin; Abdelgawad, Mohamed Essameldim; Kristensen, Helene Bjørg


    Bone remodeling requires bone resorption by osteoclasts, bone formation by osteoblasts, and a poorly investigated reversal phase coupling resorption to formation. Likely players of the reversal phase are the cells recruited into the lacunae vacated by the osteoclasts and presumably preparing...... these lacunae for bone formation. These cells, called herein reversal cells, cover >80% of the eroded surfaces, but their nature is not identified, and it is not known whether malfunction of these cells may contribute to bone loss in diseases such as postmenopausal osteoporosis. Herein, we combined...... histomorphometry and IHC on human iliac biopsy specimens, and showed that reversal cells are immunoreactive for factors typically expressed by osteoblasts, but not for monocytic markers. Furthermore, a subpopulation of reversal cells showed several distinctive characteristics suggestive of an arrested...

  10. Direct formate fuel cells: A review (United States)

    An, L.; Chen, R.


    Direct formate fuel cells (DFFC), which convert the chemical energy stored in formate directly into electricity, are recently attracting more attention, primarily because of the use of the carbon-neutral fuel and the low-cost electrocatalytic and membrane materials. As an emerging energy technology, the DFFC has made a rapid progress in recent years (currently, the state-of-the-art power density is 591 mW cm-2 at 60 °C). This article provides a review of past research on the development of this type of fuel cell, including the working principle, mechanisms and materials of the electrocatalytic oxidation of formate, singe-cell designs and performance, as well as innovative system designs. In addition, future perspectives with regard to the development of this fuel cell system are also highlighted.

  11. Heterotopic bone formation following total shoulder arthroplasty

    DEFF Research Database (Denmark)

    Kjaersgaard-Andersen, P.; Frich, Lars Henrik; Sjøbjerg, J.O.


    The incidence and location of heterotopic bone formation following total shoulder arthroplasty were evaluated in 58 Neer Mark-II total shoulder replacements. One year after surgery, 45% had developed some ectopic ossification. In six shoulders (10%) the ossifications roentgenographically bridged...... the glenohumeral and/or the glenoacromial space. There was no correlation between shoulder pain and the development of ossification. Shoulders with grade III heterotopic bone formation had a limited range of active elevation compared with shoulders without or with only a milder lesion. Men and patients...... with osteoarthritis of the shoulder joint were significantly disposed to the development of heterotopic bone. Heterotopic bone formation following total shoulder arthroplasty is frequent, but disabling heterotopic ossifications seem to be rare....

  12. Simulating Stellar Cluster Formation and Early Evolution (United States)

    Wall, Joshua; McMillan, Stephen L. W.; Mac Low, Mordecai-Mark; Ibañez-Mejia, Juan; Portegies Zwart, Simon; Pellegrino, Andrew


    We present our current development of a model of stellar cluster formation and evolution in the presence of stellar feedback. We have integrated the MHD code Flash into the Astrophysical Multi-Use Software Environment (AMUSE) and coupled the gas dynamics to an N-body code using a Fujii gravity bridge. Further we have integrated feedback from radiation using the FERVENT module for Flash, supernovae by thermal and kinetic energy injection, and winds by kinetic energy injection. Finally we have developed a method of implementing star formation using the Jeans criterion of the gas. We present initial results from our cluster formation model in a cloud using self-consistent boundary conditions drawn from a model of supernova-driven interstellar turbulence.

  13. Silver-Palladium Surfaces Inhibit Biofilm Formation

    DEFF Research Database (Denmark)

    Chiang, Wen-Chi; Schroll, Casper; Hilbert, Lisbeth Rischel


    Undesired biofilm formation is a major concern in many areas. In the present study, we investigated biofilm-inhibiting properties of a silver-palladium surface that kills bacteria by generating microelectric fields and electrochemical redox processes. For evaluation of the biofilm inhibition...... efficacy and study of the biofilm inhibition mechanism, the silver-sensitive Escherichia coli J53 and the silver-resistant E. coli J53[pMG101] strains were used as model organisms, and batch and flow chamber setups were used as model systems. In the case of the silver-sensitive strain, the silver......-palladium surfaces killed the bacteria and prevented biofilm formation under conditions of low or high bacterial load. In the case of the silver-resistant strain, the silver-palladium surfaces killed surface-associated bacteria and prevented biofilm formation under conditions of low bacterial load, whereas under...

  14. Deep learning for SAR image formation (United States)

    Mason, Eric; Yonel, Bariscan; Yazici, Birsen


    The recent success of deep learning has lead to growing interest in applying these methods to signal processing problems. This paper explores the applications of deep learning to synthetic aperture radar (SAR) image formation. We review deep learning from a perspective relevant to SAR image formation. Our objective is to address SAR image formation in the presence of uncertainties in the SAR forward model. We present a recurrent auto-encoder network architecture based on the iterative shrinkage thresholding algorithm (ISTA) that incorporates SAR modeling. We then present an off-line training method using stochastic gradient descent and discuss the challenges and key steps of learning. Lastly, we show experimentally that our method can be used to form focused images in the presence of phase uncertainties. We demonstrate that the resulting algorithm has faster convergence and decreased reconstruction error than that of ISTA.

  15. Cosmological Structure Formation: From Dawn till Dusk

    DEFF Research Database (Denmark)

    Heneka, Caroline Samantha

    with massive galaxy clusters, traveling from the dawn of structure formation, when the first galaxies appear, to its dusk, when a representative part of the mass in the Universe is settled in massive structures. This hunt for accurate constraints on cosmology is complemented with the demonstration of novel......Cosmology has entered an era where a plethora data is available on structure formation to constrain astrophysics and underlying cosmology. This thesis strives to both investigate new observables and modeling of the Epoch of Reionization, as well as to constrain dark energy phenomenology...... Bayesian statistical tools and kinematical constraints on dark energy. Starting at the dawn of structure formation, we study emission line fluctuations, employing semi-numerical simulations of cosmological volumes of their line emission, in order to cross-correlate fluctuations in brightness. This cross...

  16. Exploring the value of usability feedback formats

    DEFF Research Database (Denmark)

    Nørgaard, Mie; Hornbæk, Kasper Anders Søren


    presented using five feedback formats. Our usability findings comprise 35 problems and 5 positive comments. Data suggest that feedback serves multiple purposes. Initially, feedback must convince developers about the relevance of a problem and convey an understanding of this. Feedback must next be easy......The format used to present feedback from usability evaluations to developers affects whether problems are understood, accepted, and fixed. Yet, little research has investigated which formats are the most effective. We describe an explorative study where three developers assess 40 usability findings...... to use and finally serve as a reminder of the problem. Prior to working with the feedback, developers rated redesign proposals, multimedia reports, and annotated screen dumps as more valuable than lists of problems, all of which were rated as more valuable than scenarios. After having spent some time...

  17. Hot subdwarf formation: Confronting theory with observation

    Directory of Open Access Journals (Sweden)

    Geier S.


    Full Text Available The formation of hot subdwarf stars is still unclear. Both single-star and binary scenarios have been proposed to explain the properties of these evolved stars situated at the extreme blue end of the horizontal branch. The observational evidence gathered in the last decade, which revealed high fractions of binaries, shifted the focus from the single-star to the binary formation scenarios. Common envelope ejection, stable Roche lobe overflow and the merger of helium white dwarfs seemed to be sufficient to explain the formation of both the binary as well as the remaining single hot subdwarfs. However, most recent and rather unexpected observations challenge the standard binary evolution scenarios.


    Energy Technology Data Exchange (ETDEWEB)

    Hunter, Deidre A.; Ashburn, Allison; Wright, Teresa [Lowell Observatory, 1400 West Mars Hill Road, Flagstaff, AZ 86001 (United States); Elmegreen, Bruce G. [IBM T. J. Watson Research Center, P.O. Box 218, Yorktown Heights, NY 10598 (United States); Rubin, Vera C. [Carnegie Institution of Washington, 5241 Broad Branch Road NW, Washington, DC 20015 (United States); Józsa, Gyula I. G.; Struve, Christian [ASTRON (Netherlands Organisation for Scientific Research NWO), Oude Hoogeveensedijk 4, 7991-PD Dwingeloo (Netherlands)


    We examined star formation in two very luminous (M{sub V} = –22 to –23) Sc-type spiral galaxies, NGC 801 and UGC 2885, using ultra-deep Hα images. We combine these Hα images with UBV and Two-Micron All-Sky Survey JHK images and H I maps to explore the star formation characteristics of disk galaxies at high luminosity. Hα traces star formation in these galaxies to 4-6 disk scale lengths, but the lack of detection of Hα further out is likely due to the loss of Lyman continuum photons. Considering gravitational instabilities alone, we find that the gas and stars in the outer regions are marginally stable in an average sense, but considering dissipative gas and radial and azimuthal forcing, the outer regions are marginally unstable to forming spiral arms. Star formation is taking place in spiral arms, which are regions of locally higher gas densities. Furthermore, we have traced smooth exponential stellar disks over four magnitudes in V-band surface brightness and 4-6 disk scale lengths, in spite of a highly variable gravitational instability parameter. Thus, gravitational instability thresholds do not seem relevant to the stellar disk. One possibility for creating an exponential disk is that the molecular cloud densities and star formation rates have exponential profiles and this fact forces the stellar disk to build up such a profile. Another possibility is that the stellar disk is continuously adjusted to an exponential shape regardless of the star formation profile, for example, through global dynamical processes that scatter stars. However, such scattering processes are only known to operate in spiral systems, in which case they cannot explain the same dilemma of smooth exponential disks observed in dwarf irregular galaxies.

  19. An existentialist account of identity formation. (United States)

    Bilsker, D


    An account of James Marcia's identity formation model is given in the language of existentialist philosophy. Parallels between ego-identity and existentialist approaches are examined and identity is described in terms of existentialist concepts formulated by Martin Heidegger (Being and Time) and Jean-Paul Sartre (Being and Nothingness). While identity formation has previously been formulated in terms of ego-analytic theory, this paper argues and seeks to demonstrate that significant benefits accrue from an existentialist account. These benefits include clarification of difficult theoretical issues, delineation of specific research directions and enrichment of clinical understanding.

  20. Aromatics oxidation and soot formation in flames

    Energy Technology Data Exchange (ETDEWEB)

    Howard, J.B.; Pope, C.J.; Shandross, R.A.; Yadav, T. [Massachusetts Institute of Technology, Cambridge (United States)


    This project is concerned with the kinetics and mechanisms of aromatics oxidation and soot and fullerenes formation in flames. The scope includes detailed measurements of profiles of stable and radical species concentrations in low-pressure one-dimensional premixed flames. Intermediate species identifications and mole fractions, fluxes, and net reaction rates calculated from the measured profiles are used to test postulated reaction mechanisms. Particular objectives are to identify and to determine or confirm rate constants for the main benzene oxidation reactions in flames, and to characterize fullerenes and their formation mechanisms and kinetics.

  1. Pratiques sociales en formation à distance

    Directory of Open Access Journals (Sweden)

    Clément Dussarps


    Full Text Available L’abandon en formation ouverte et à distance (FOAD constitue un enjeu important tant pour les apprenants que pour les organismes de formation, que ce soit pour des raisons économiques, sociales, ou encore d’image de soi ou publique (Sauvé, 2012. L’un des éléments clés de l’abandon semble être la solitude vécue par les apprenants (Glikman, 2002, p. 242. Ce sentiment de solitude et l’abandon semblent peu dépendants de facteurs socio-démographiques (sexe, âge, etc. (Park et Choi, 2009. Face...

  2. Pratiques sociales en formation à distance


    Clément Dussarps; Didier Paquelin


    L’abandon en formation ouverte et à distance (FOAD) constitue un enjeu important tant pour les apprenants que pour les organismes de formation, que ce soit pour des raisons économiques, sociales, ou encore d’image de soi ou publique (Sauvé, 2012). L’un des éléments clés de l’abandon semble être la solitude vécue par les apprenants (Glikman, 2002, p. 242). Ce sentiment de solitude et l’abandon semblent peu dépendants de facteurs socio-démographiques (sexe, âge, etc.) (Park et Choi, 2009). Face...

  3. Induction heaters used to heat subsurface formations (United States)

    Nguyen, Scott Vinh [Houston, TX; Bass, Ronald M [Houston, TX


    A heating system for a subsurface formation includes an elongated electrical conductor located in the subsurface formation. The electrical conductor extends between at least a first electrical contact and a second electrical contact. A ferromagnetic conductor at least partially surrounds and at least partially extends lengthwise around the electrical conductor. The electrical conductor, when energized with time-varying electrical current, induces sufficient electrical current flow in the ferromagnetic conductor such that the ferromagnetic conductor resistively heats to a temperature of at least about C.

  4. Pathways to stepfamily formation in Europe

    Directory of Open Access Journals (Sweden)


    Full Text Available Increasing proportions of couples are making childbearing decisions in stepfamilies but there has been no general comparative picture across European countries on stepfamily formation. The present paper aims to fill this gap and provides a comparison of European countries using macro-level indicators that describe union formation and dissolution and childbearing. We use the individual-level data files (standard recode files of Fertility and Family Surveys from 19 European countries. Our results highlight the different pathways to a stepfamily in Europe, and show that in most European countries a considerable proportion of women form a stepfamily in childbearing ages, which needs to be considered in studies of fertility.

  5. The formation of daodi medicinal materials. (United States)

    Zhao, Zhongzhen; Guo, Ping; Brand, Eric


    Daodi medicinal material is produced and assembled in specific geographic regions with designated natural conditions and ecological environment, with particular attention to cultivation technique, harvesting and processing. The quality and clinical effects surpass those of same botanical origin produced from other regions. It is thus widely recognized and has long enjoyed a good reputation. Based on literature, market and field investigation on daodi medicinal materials, the historical background and reasons behind the formation and the development of daodi medicinal material are analyzed. This review clarifies the concept and rationalizes the formation of daodi medicinal material. Copyright © 2012 Elsevier Ireland Ltd. All rights reserved.

  6. Group formation and ideology. Text and context. (United States)

    Hernandez, M


    The relationship between the phenomenon of 'mass formation' (Massenbildung) and the production and circulation of ideologies is examined. The explanation of the said relationship must take into account both the social dimension as well as the intrapsychic structure of the ideological. To achieve this a brief review of Freud's ideas on group psychology and of some definitions of ideology, namely those proposed by Ricoeur, Althusser and Habermas is expounded. In the same way as the understanding of the vicissitudes of identification is crucial to gain insight into 'mass formation', the investigation of the processes of idealization is vital to the understanding of the ideological.

  7. Multilevel Modulation formats for Optical Communication

    DEFF Research Database (Denmark)

    Jensen, Jesper Bevensee


    This thesis studies the use of multilevel modulation formats for optical communication systems. Multilevel modulation is an attractive method of increasing the spectral efficiency of optical communication systems. Various modulation formats employing phase modulation, amplitude modulation...... or a combination of the two have been studied. The use of polarization multiplexing (PolMux) to double the bit rate has also been investigated. The impact of transmission impairments such as chromatic dispersion, self phase modulation and cross phase modulation has been investigated. The feasibility of multilevel...... modulation for network oriented scenarios has been demonstrated....

  8. Formation of acrylamide in cheese bread

    DEFF Research Database (Denmark)

    Hedegaard, Rikke Susanne Vingborg; Sobrinho, Luis Gualberto De Andrade; Granby, Kit


    of cheese added, and addition of 23.7 g cheese resulted in 958 ppb acrylamide. For an o/w rapeseed oil emulsion as a food model heated under conditions similar to those persisting inside bread during baking, it was further shown that acrylamide formation also occurred in absence of reducing sugars....... In contrast, acrylamide was not observed in Pao de queijo a traditional Brazilian bread product made from fermented cassava flour, fresh eggs and a mixture of Brazilian Gouda type cheese and Mozzarella cheese pointing towards a role of eggs in protection against acrylamide formation....

  9. Thermally activated martensite formation in ferrous alloys

    DEFF Research Database (Denmark)

    Villa, Matteo; Somers, Marcel A. J.


    Magnetometry was applied to investigate the formation of α/α´martensite in 13ferrous alloys during immersion in boiling nitrogen and during re-heating to room temperature at controlled heating rates in the range 0.0083-0.83 K s-1. Data showsthat in 3 of the alloys, those that form {5 5 7}γ...... martensite, no martensite developsduring cooling. For all investigated alloys, irrespective of the type of martensiteforming, thermally activated martensite develops during heating. The activationenergy for thermally activated martensite formation is in the range 8‒27 kJ mol-1and increases with the fraction...... of interstitial solutes in the alloy...

  10. Structure formation cosmic rays: Identifying observational constraints


    Prodanović T.; Fields B.D.


    Shocks that arise from baryonic in-fall and merger events during the structure formation are believed to be a source of cosmic rays. These "structure formation cosmic rays" (SFCRs) would essentially be primordial in composition, namely, mostly made of protons and alpha particles. However, very little is known about this population of cosmic rays. One way to test the level of its presence is to look at the products of hadronic reactions between SFCRs and the ISM. A perfect probe of these react...

  11. Nucler star formation in NGC 6240


    Pasquali, A.; Gallagher, J. S.; de Grijs, R.


    We have made use of archival HST BVIJH photometry to constrain the nature of the three discrete sources, A1, A2 and B1, identified in the double nucleus of NGC 6240. STARBURST99 models have been fitted to the observed colours, under the assumption, first, that these sources can be treated as star clusters (i.e. single, instantaneous episodes of star formation), and subsequently as star-forming regions (i.e. characterised by continuous star formation). For both scenarios, we estimate ages as y...

  12. Functions in Free-Format RPG IV

    CERN Document Server

    Martin, Jim


    Written especially for programmers adopting a free-format style, this manual explores the role of functions in writing RPG IV programs. Demonstrating the potential of functions, many topics are explored such as details about existing RPG IV built-in functions, writing new functions, using ILE concepts to use C functions, and utilizing IBM API's functions. Explaining how to write small programs, either as sub-procedures or modules, and how to gather those parts together to make programs that are easy to write and maintain, this is a natural next step for programmers familiar with a free-format

  13. Simulation of Kinkband Formation in Fiber Composites

    DEFF Research Database (Denmark)

    Veluri, Badri; Jensen, Henrik Myhre


    Failure of composite materials by the formation of kinkbands is a non-linear phenomenon involving interacting non-linear effects of material behavior of the matrix materials and fiber buckling. A constitutive model for unidirectional layered materials is implemented as User Material (UMAT) user...... subroutine in ABAQUS/Standard for analyzing the kinkband formation in the fiber composites under compressive loading within the framework of large deformation kinematics. This computational model analyses the effects of misalignment on elastic plastic deformation under plane strain conditions based...

  14. Embedded Clusters: Laboratories for Star Formation (United States)

    Lada, E. A.


    Embedded clusters are the fundamental units of star formation in our Galaxy, therefore studying their properties is critical for understanding how star formation proceeds on both the local and Galactic scale. We have surveyed embedded clusters in local molecular clouds with FLAMINGOS and are investigating the star forming histories, IMF, structure and evolution of these young clusters. In this presentation, I will discuss our results for the clusters in the Rosette and Orion star forming complexes where we find evidence for the evolution of cluster structure and variations in the low mass end of the initial mass function.

  15. No effect of steroids on seroma formation after mastectomy

    DEFF Research Database (Denmark)

    Okholm, Mette; Axelsson, Christen Kirk


    Seroma formation is a common problem after breast surgery. Studies indicate that seroma formation is a result of the postoperative inflammatory process. Glucocorticoid inhibits the inflammatory response....

  16. Comparison of jeopardy game format versus traditional lecture format as a teaching methodology in medical education. (United States)

    Khan, Mohammad N; Telmesani, Abdulwahab; Alkhotani, Abdulaziz; Elzouki, Abdelaziz; Edrees, Burhan; Alsulimani, Mohammad H


    To compare students` performance, satisfaction, and retention of knowledge between a `jeopardy game format` and a `didactic lecture format` in teaching viral exanthema to fifth-year medical students. We conducted a parallel-group randomized controlled trial in the Department of Pediatrics, Faculty of Medicine, Umm Al-Qura University, Makkah, Saudi Arabia from November 2008 to January 2009. We randomized fifth-year medical students into 2 groups. We taught viral exanthema to group one in lecture format, while group 2 received the same instruction in a jeopardy style game format. Both groups underwent a pretest, post-test I, and satisfaction survey. We conducted post-test II after 2 months to assess the retention of knowledge. The satisfaction survey consisted of 5 questions using a 5 point Likert scale. We used the paired sample t-test, and independent sample t-test to compare the results. Eighty-two students participated in the study (41 in each group). Both groups showed significant improvement in their knowledge on the post-test I compared with the pre-test scores. However, the post-test II conducted after 2 months showed that retention of knowledge was significantly better in the game format. The satisfaction survey showed that the game format was more enjoyable and fun. The game format teaching strategy has an added advantage in retaining knowledge of the subject for a longer time compared with a lecture format.

  17. Perceptions and practices of U.S. dental schools regarding curriculum integrated format and traditional format licensure exams

    National Research Council Canada - National Science Library

    Desai, Shamik; Allareddy, Veerasathpurush; Donoff, R Bruce; Howell, T Howard; Karimbux, Nadeem Y


    The dental licensure exam in the United States has evolved over the past ten years, and two formats-the traditional format and curriculum integrated format-are now available for students to satisfy...

  18. Defect formation in long Josephson junctions

    DEFF Research Database (Denmark)

    Gordeeva, Anna; Pankratov, Andrey


    We study numerically a mechanism of vortex formation in a long Josephson junction within the framework of the one-dimensional sine-Gordon model. This mechanism is switched on below the critical temperature. It is shown that the number of fluxons versus velocity of cooling roughly scales according...

  19. Gas Accretion and Star Formation Rates (United States)

    Sánchez Almeida, Jorge

    Cosmological numerical simulations of galaxy evolution show that accretion of metal-poor gas from the cosmic web drives the star formation in galaxy disks. Unfortunately, the observational support for this theoretical prediction is still indirect, and modeling and analysis are required to identify hints as actual signs of star formation feeding from metal-poor gas accretion. Thus, a meticulous interpretation of the observations is crucial, and this observational review begins with a simple theoretical description of the physical process and the key ingredients it involves, including the properties of the accreted gas and of the star formation that it induces. A number of observations pointing out the connection between metal-poor gas accretion and star formation are analyzed, specifically, the short gas-consumption time-scale compared to the age of the stellar populations, the fundamental metallicity relationship, the relationship between disk morphology and gas metallicity, the existence of metallicity drops in starbursts of star-forming galaxies, the so-called G dwarf problem, the existence of a minimum metallicity for the star-forming gas in the local universe, the origin of the α-enhanced gas forming stars in the local universe, the metallicity of the quiescent BCDs, and the direct measurements of gas accretion onto galaxies. A final section discusses intrinsic difficulties to obtain direct observational evidence, and points out alternative observational pathways to further consolidate the current ideas.

  20. Austenite formation in C-Mn steel

    NARCIS (Netherlands)

    Savran, V.I.


    The production process of almost all modern steels involves austenitization formation of the austenite phase upon continuous heating. Many of the microstructural features and properties that are obtained upon subsequent cooling are to a large extend determined by the evolution of the microstructure

  1. Fibrin clot formation and lysis: basic mechanisms

    DEFF Research Database (Denmark)

    Sidelmann, JJ; Gram, J; Jespersen, J


    consequently is an important substrate in the physiology of hemostasis. This review describes the components and processes involved in fibrin formation and fibrin degradation. Particular emphasis is put on the reactions involved in the conversion of fibrinogen to fibrin, the polymerization of fibrin molecules...

  2. Assessing Migration Risk for Scientific Data Formats

    Directory of Open Access Journals (Sweden)

    Chris Frisz


    Full Text Available The majority of information about science, culture, society, economy and the environment is born digital, yet the underlying technology is subject to rapid obsolescence. One solution to this obsolescence, format migration, is widely practiced and supported by many software packages, yet migration has well known risks. For example, newer formats – even where similar in function – do not generally support all of the features of their predecessors, and, where similar features exist, there may be significant differences of interpretation.There appears to be a conflict between the wide use of migration and its known risks. In this paper we explore a simple hypothesis – that, where migration paths exist, the majority of data files can be safely migrated leaving only a few that must be handled more carefully – in the context of several scientific data formats that are or were widely used. Our approach is to gather information about potential migration mismatches and, using custom tools, evaluate a large collection of data files for the incidence of these risks. Our results support our initial hypothesis, though with some caveats. Further, we found that writing a tool to identify “risky” format features is considerably easier than writing a migration tool.

  3. Crack Formation in Grouted Annular Composite

    DEFF Research Database (Denmark)

    Sørensen, Eigil V.

    The objective of the present analysis is to identify the reason for extensive crack formation which occurred during an annulus grouting performance test, to evaluate possible consequences of the cracking, and to recommend measures to be taken in order to avoid similar problems in the future....

  4. Activation of PAD4 in NET formation

    Directory of Open Access Journals (Sweden)

    Amanda eRohrbach


    Full Text Available Peptidyl arginine deiminases, or PADs, convert arginine residues to the non-ribosomally encoded amino acid citrulline in a variety of protein substrates. PAD4 is expressed in granulocytes and is essential for the formation of neutrophil extracellular traps (NETs via PAD4-mediated histone citrullination. Citrullination of histones is thought to promote NET formation by inducing chromatin decondensation and facilitating the expulsion of chromosomal DNA that is coated with antimicrobial molecules. Numerous stimuli have been reported to lead to PAD4 activation and NET formation. However, how this signaling process proceeds and how PAD4 becomes activated in cells is largely unknown. Herein, we describe the various stimuli and signaling pathways that have been implicated in PAD4 activation and NET formation, including the role of reactive oxygen species generation. To provide a foundation for the above discussion, we first describe PAD4 structure and function, and how these studies led to the development of PAD-specific inhibitors. A comprehensive survey of the receptors and signaling pathways that regulate PAD4 activation will be important for our understanding of innate immunity, and the identification of signaling intermediates in PAD4 activation may also lead to the generation of pharmaceuticals to target NET-related pathogenesis.

  5. MPEG-7 multimedia-based query format (United States)

    Chang, Wo


    Searching multimedia content for image, audio, and video is getting more attention especially for personal media content due to the affordability of consumer electronic devices such as MP3 recordable players, digital cameras, DV camcorders, and well-integrated smart phones. The precise search and retrieval of the content derived from these devices can be a very challenging task. Many leading edge search engine vendors have been applying sophisticated and advanced indexing and retrieval techniques on various text-based document formats, but when it comes to retrieving multimedia content, searching based on the media clip filename is the most common practice. As a result, there is an imprecise and ineffective user experience for searching multimedia content. This paper presents a new development underway from a joint effort between International Organization for Standardization (ISO)/International Electrotechnial Commission (IEC) Subcommittee (SC) 29 Working Group (WG) 11 MPEG (Moving Picture Experts Group) and WG1 JPEG (Joint Picture Experts Group) for a universal standard query format called MPEG-7 Query Format (MP7QF) as a means to enable a good user experience for consumers searching multimedia content. It also provides the industry with a unified way to accept and respond to user queries. This paper presents the core requirements for such a universal query format.

  6. On the formation of Caldera’s

    NARCIS (Netherlands)

    Escher, B.G.


    In two previous publications (bibl. 1 and 2) I have brought the formation of calderas into relation with the gas phase, observed by Perret during the eruption of Vesuvius in 1906 (bibl. 3). In these papers I arrived at the conclusion that during the gas phase a cylinder is cored out, and that this

  7. The dynamics of group formation among leeches

    Directory of Open Access Journals (Sweden)

    Giacomo eBisson


    Full Text Available Leeches exploring a new environment continuously meet each other and merge in temporary groups. After 2-3 hours, leeches become attracted to each other eventually forming a large and stable group. When their number is reduced, leeches remain solitary, behaving independently. Group formation is facilitated by body injection of serotonin (5-HT and the level of endogenous 5-HT is elevated in leeches forming a large group. In contrast, intravenous injection of 5-HT antagonists prevented injected leeches from joining a large group of conspecifics. When sensilla near the head were ablated or the supraesophageal ganglion disconnected, leeches remained solitary, but explored the environment swimming and crawling. These results suggest that group formation is initiated by a release of 5-HT triggered by sensilla stimulation and its dynamics can be explained by the establishment of a reinforcement dynamics, as observed during human group formation. As 5-HT affects social interactions also in humans, group formation in leeches and humans share a similar dynamics and hormonal control.

  8. Does simvastatin stimulate bone formation in vivo?

    Directory of Open Access Journals (Sweden)

    Chorev Michael


    Full Text Available Abstract Background Statins, potent compounds that inhibit cholesterol synthesis in the liver have been reported to induce bone formation, both in tissue culture and in rats and mice. To re-examine potential anabolic effects of statins on bone formation, we compared the activity of simvastatin (SVS to the known anabolic effects of PTH in an established model of ovariectomized (OVX Swiss-Webster mice. Methods Mice were ovariectomized at 12 weeks of age (T0, remained untreated for 5 weeks to allow development of osteopenia (T5, followed by treatment for 8 weeks (T13. Whole, trabecular and cortical femoral bone was analyzed by micro-computed tomography (micro CT. Liquid chromatography/mass spectrometry (LC/MS was used to detect the presence of SVS and its active metabolite, simvastatin β-hydroxy acid (SVS-OH in the mouse serum. Results Trabecular BV/TV at T13 was 4.2 fold higher in animals treated with PTH (80 micro-g/kg/day compared to the OVX-vehicle treated group (p in vivo study. Conclusions While PTH demonstrated the expected anabolic effect on bone, SVS failed to stimulate bone formation, despite our verification by LC/MS of the active SVS-OH metabolite in mouse serum. While statins have clear effects on bone formation in vitro, the formulation of existing 'liver-targeted' statins requires further refinement for efficacy in vivo.

  9. Music and Identity Formation in Older Adults (United States)

    Dabback, William M.


    For many individuals, engagement with the arts and music in later life plays a significant role in identity formation. Research indicates that music engagement facilitates successful aging. While participants may or may not establish identities as musicians, musical activities provide interactions and opportunities that facilitate their…

  10. Quilting after mastectomy significantly reduces seroma formation ...

    African Journals Online (AJOL)

    Introduction: Seroma formation is one of the most frequently encountered complications following mastectomy. It may cause significant morbidity, including delayed wound healing, infection and frequent clinic attendance for seroma aspiration. Objective: To evaluate the effect of surgical quilting after mastectomy in the ...

  11. Star formation history written in spectra

    NARCIS (Netherlands)

    Ellerbroek, L.E.


    In this thesis, the process of star formation is mapped from large to small scales, using the world's most advanced observatories. Discoveries of several young stars with peculiar environments are reported. Dynamics of circumstellar gas and dust are analyzed in a diverse ensemble of young stars. The

  12. Pattern formation in Pseudomonas aeruginosa biofilms

    DEFF Research Database (Denmark)

    Parsek, Matthew R.; Tolker-Nielsen, Tim


    Bacteria are capable of forming elaborate multicellular communities called biofilms. Pattern formation in biofilms depends on cell proliferation and cellular migration in response to the available nutrients and other external cues, as well as on self-generated intercellular signal molecules and t...

  13. Formation of multiple dark photovoltaic spatial solitons

    Indian Academy of Sciences (India)

    We theoretically study the formation of multiple dark photovoltaic soliton splitting in the quasi-steady-state and steady-state regimes under open-circuit conditions. We find that the initial width of the dark notch at the entrance face of the crystal is a key parameter for generating an even (or odd) number sequence of dark ...

  14. Memory, expectation formation and scheduling choices

    NARCIS (Netherlands)

    Koster, P.R.; Peer, S.; Dekker, T.


    Limited memory capacity, retrieval constraints and anchoring are central to expectation formation processes. We develop a model of adaptive expectations where individuals are able to store only a finite number of past experiences of a stochastic state variable. Retrieval of these experiences is

  15. Experimental evidence on inflation expectation formation

    NARCIS (Netherlands)

    Pfajfar, D.; Zakelj, B.

    Using laboratory experiments within a New Keynesian sticky price framework, we study the process of inflation expectation formation. We focus on adaptive learning and rational expectations contrary to the previous literature that mostly studied simple heuristics. Using a test for rational

  16. When binding is enough: nonactivating antibody formats

    NARCIS (Netherlands)

    Labrijn, Aran F.; Aalberse, Rob C.; Schuurman, Janine


    Most therapeutic antibodies currently used in the clinic are based on the human IgG1 format, which is a bivalent molecule that efficiently interacts with the immune system's effector functions. In clinical applications where binding to the target alone is sufficient for therapeutic efficacy;

  17. Multigenerational Perspectives on Family Formation Behaviour

    DEFF Research Database (Denmark)

    Loft, Lisbeth Trille Gylling


    life. Moreover, presence of generational transmission of family formation behavior such as cohabitation, age at first marriage and childbearing has been widely documented. Thus, it has become more appropriate to approach family life events in a non-linear manner by emphasizing the existence of various...

  18. Formative assessment promotes learning in undergraduate clinical ...

    African Journals Online (AJOL)

    Introduction. Clinical clerkships, typically situated in environments lacking educational structure, form the backbone of undergraduate medical training. The imperative to develop strategies that enhance learning in this context is apparent. This study explored the impact of longitudinal bedside formative assessment on ...

  19. Molecular genetics of supernumerary tooth formation. (United States)

    Wang, Xiu-Ping; Fan, Jiabing


    Despite advances in the knowledge of tooth morphogenesis and differentiation, relatively little is known about the aetiology and molecular mechanisms underlying supernumerary tooth formation. A small number of supernumerary teeth may be a common developmental dental anomaly, while multiple supernumerary teeth usually have a genetic component and they are sometimes thought to represent a partial third dentition in humans. Mice, which are commonly used for studying tooth development, only exhibit one dentition, with very few mouse models exhibiting supernumerary teeth similar to those in humans. Inactivation of Apc or forced activation of Wnt/β(catenin signalling results in multiple supernumerary tooth formation in both humans and in mice, but the key genes in these pathways are not very clear. Analysis of other model systems with continuous tooth replacement or secondary tooth formation, such as fish, snake, lizard, and ferret, is providing insights into the molecular and cellular mechanisms underlying succesional tooth development, and will assist in the studies on supernumerary tooth formation in humans. This information, together with the advances in stem cell biology and tissue engineering, will pave ways for the tooth regeneration and tooth bioengineering. Copyright © 2011 Wiley-Liss, Inc.

  20. Cu(II) promotes amyloid pore formation

    Energy Technology Data Exchange (ETDEWEB)

    Zhang, Hangyu, E-mail: [Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN 47907 (United States); Rochet, Jean-Christophe [Department of Medicinal Chemistry and Molecular Pharmacology, Purdue University, West Lafayette, IN 47907 (United States); Stanciu, Lia A. [Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN 47907 (United States); School of Materials Engineering, Purdue University, West Lafayette, IN 47907 (United States)


    The aggregation of α-synuclein is associated with dopamine neuron death in Parkinson's disease. There is controversy in the field over the question of which species of the aggregates, fibrils or protofibrils, are toxic. Moreover, compelling evidence suggested the exposure to heavy metals to be a risk of PD. Nevertheless, the mechanism of metal ions in promoting PD remains unclear. In this research, we investigated the structural basis of Cu(II) induced aggregation of α-synuclein. Using transmission electron microscopy experiments, Cu(II) was found to promote in vitro aggregation of α-synuclein by facilitating annular protofibril formation rather than fibril formation. Furthermore, neuroprotective baicalein disaggregated annular protofibrils accompanied by considerable decrease of β-sheet content. These results strongly support the hypothesis that annular protofibrils are the toxic species, rather than fibrils, thereby inspiring us to search novel therapeutic strategies for the suppression of the toxic annular protofibril formation. - Highlights: • Cu(II) promoted the annular protofibril formation of α-synuclein in vitro. • Cu(II) postponed the in vitro fibrillization of α-synuclein. • Neuroprotective baicalein disaggregated annular protofibrils.

  1. Star formation history of barred disc galaxies

    NARCIS (Netherlands)

    Sanchez-Blazquez, P.; Ocvirk, P.; Gibson, B. K.; Perez, I.; Peletier, R. F.

    We present the first results of a pilot study aimed at understanding the influence of bars on the evolution of galaxy discs through the study of their stellar content. We examine here the kinematics, star formation history, mass-weighted, luminosity-weighted and single stellar population (SSP)

  2. Generalized Controller for Directed Triangle Formations

    NARCIS (Netherlands)

    Cao, M.; Yu, C.; Morse, A.S.; Anderson, B.D.O.; Dasgupta, S.


    This paper proposes and analyzes a distributed control law which generalizes three different previously considered control laws for maintaining a triangular formation in the plane consisting of three point-modelled, mobile autonomous agents. It is shown that the control law under consideration can

  3. Formative Assessment in Mathematics for Engineering Students (United States)

    Ní Fhloinn, Eabhnat; Carr, Michael


    In this paper, we present a range of formative assessment types for engineering mathematics, including in-class exercises, homework, mock examination questions, table quizzes, presentations, critical analyses of statistical papers, peer-to-peer teaching, online assessments and electronic voting systems. We provide practical tips for the…

  4. The Star Formation History of RCW 36

    NARCIS (Netherlands)

    Ellerbroek, L. E.; Kaper, L.; Bik, A.; Maaskant, K. M.; Podio, L.; Carciofi, A.; Rivinius, Th.


    Recent studies of massive-star forming regions indicate that they can contain multiple generations of young stars. These observations suggest that star formation in these regions is sequential and/or triggered by a previous generation of (massive) stars. Here we present new observations of the star

  5. Formation of Massive Stars: Theoretical Considerations (United States)

    Yorke, Harold W.


    This slide presentation reviews theoretical considerations of the formation of massive stars. It addresses the questions that assuming a gravitationally unstable massive clump, how does enough material become concentrated into a sufficiently small volume within a sufficiently short time? and how does the forming massive star influence its immediate surroundings to limit its mass?

  6. The Policy Dispositif: Historical Formation and Method (United States)

    Bailey, Patrick L. J.


    This paper proposes a new way of conceptualising education policy and also begins to develop a new method of policy analysis. In both instances, it draws on the theoretical and conceptual tools of Foucault, and in particular his concept "dispositif." It posits an historical and ontological formation -- a policy dispositif -- with…

  7. Star Formation in Merging Galaxies Using FIRE (United States)

    Perez, Adrianna; Hung, Chao-Ling; Naiman, Jill; Moreno, Jorge; Hopkins, Philip


    Galaxy interactions and mergers are efficient mechanisms to birth stars at rates that are significantly higher than found in our Milky Way galaxy. The Kennicut-Schmidt (KS) relation is an empirical relationship between the star-forming rate and gas surface densities of galaxies (Schmidt 1959; Kennicutt 1998). Although most galaxies follow the KS relation, the high levels of star formation in galaxy mergers places them outside of this otherwise tight relationship. The goal of this research is to analyze the gas content and star formation of simulated merging galaxies. Our work utilizes the Feedback In Realistic Environments (FIRE) model (Hopkins et al., 2014). The FIRE project is a high-resolution cosmological simulation that resolves star-forming regions and incorporates stellar feedback in a physically realistic way. In this work, we have noticed a significant increase in the star formation rate at first and second passage, when the two black holes of each galaxy approach one other. Next, we will analyze spatially resolved star-forming regions over the course of the interacting system. Then, we can study when and how the rates that gas converts into stars deviate from the standard KS. These analyses will provide important insights into the physical mechanisms that regulate star formation of normal and merging galaxies and valuable theoretical predictions that can be used to compare with current and future observations from ALMA or the James Webb Space Telescope.

  8. Undergraduate Students' Mental Models of Hailstone Formation (United States)

    Cin, Mustafa


    The objective of this research is to investigate students' mental models of the hailstone formation and explore factors that may affect their mental models. The sample chosen for the study was composed of a total of 84 students. The students attended the 1st to 4 th grade classes of the Social Studies Teaching Programme at Giresun University in…

  9. The formation of planets in circumbinary discs (United States)

    Pelupessy, F. I.; Portegies Zwart, S.


    We examine the formation of planets around binary stars in light of the recently discovered systems Kepler 16, 34 and 35. We conduct hydrodynamical simulations of self-gravitating discs around binary systems. The selected binary and disc parameters are chosen consistent with observed systems. The discs are evolved until they settle in a quasi-equilibrium and the resulting systems are compared with the parameters of Kepler 16, 34 and 35. We find a close correspondence of the peak density at the inner disc gap and the orbit of the observed planets. We conclude, based on our simulations, that the orbits of the observed Kepler planets are determined by the size of the inner disc gap which for these systems results from the binary driving. This mediates planet formation either through the density enhancement or through planetary trapping at the density gradient inversion in the inner disc. For all three systems the current eccentricity of the planetary orbit is less than the disc eccentricity in the simulations. This, together with the long-term stability of the orbits argues against in situ formation (e.g. a direct collapse scenario of the material in the ring). Conducting additional simulations of systems with a wider range of parameters (taken from a survey of eclipsing binaries), we find that the planet semimajor axis and binary eccentricity in such a scenario should be tightly correlated providing an observational test of this formation mechanism.

  10. Modelling the formation of double white dwarfs

    NARCIS (Netherlands)

    van der Sluijs, M.V.; Verbunt, F.W.M.|info:eu-repo/dai/nl/068970374; Pols, O.|info:eu-repo/dai/nl/111811155


    We investigate the formation of the ten double-lined double white dwarfs that have been observed so far. A detailed stellar evolution code is used to calculate grids of single-star and binary models and we use these to reconstruct possible evolutionary scenarios. We apply various criteria to select

  11. Sambamba : Fast processing of NGS alignment formats

    NARCIS (Netherlands)

    Tarasov, Artem; Vilella, Albert J.; Cuppen, Edwin; Nijman, Isaac J.; Prins, Pjotr


    Summary: Sambamba is a high-performance robust tool and library for working with SAM, BAM and CRAM sequence alignment files; the most common file formats for aligned next generation sequencing data. Sambamba is a faster alternative to samtools that exploits multi-core processing and dramatically

  12. Sambamba : fast processing of NGS alignment formats

    NARCIS (Netherlands)

    Tarasov, Artem; Vilella, Albert J; Cuppen, Edwin; Nijman, Isaac J; Prins, Pjotr


    Sambamba is a high-performance robust tool and library for working with SAM, BAM and CRAM sequence alignment files; the most common file formats for aligned next generation sequencing data. Sambamba is a faster alternative to samtools that exploits multi-core processing and dramatically reduces

  13. Quilting after mastectomy significantly reduces seroma formation

    African Journals Online (AJOL)

    Patients who had a skin-sparing mastectomy with immediate breast reconstruction were excluded. Data were collected on .... of adjuvant treatment was made after discussion in a multidisciplinary team meeting. .... seroma formation with the use of tetracycline sclerotherapy[26] or 95% ethyl alcohol or povidone iodine.[27].

  14. The Formation of a Planetary Nebula. (United States)

    Harpaz, Amos


    Proposes a scenario to describe the formation of a planetary nebula, a cloud of gas surrounding a very hot compact star. Describes the nature of a planetary nebula, the number observed to date in the Milky Way Galaxy, and the results of research on a specific nebula. (MDH)

  15. GSK-3β and Memory Formation

    Directory of Open Access Journals (Sweden)

    Akihiko eTakashima


    Full Text Available In Alzheimer’s disease (AD, tau hyperphosphorylation and neurofibrillary tangle (NFT formation are strongly associated with dementia. Memory impairment is a characteristic, early symptom of AD. Glycogen synthase kinase 3 β (GSK-3β, which is activated in response to amyloid β (Aβ formation, and the normal process of aging, hyperphosphorylates tau present in the NFTs. Furthermore, activation of GSK-3β inhibits synaptic long-term potentiation (LTP through tau. It is therefore likely, that activation of GSK-3β is responsible for the memory problems seen in both advanced age, and AD. Indeed, inhibition of GSK-3 by lithium halts the progression of symptoms in patients with mild cognitive impairment (MCI. However, long-term treatment of lithium increases the risk of dementia in old age, in bipolar patients. To understand the role of GSK-3β in brain function, we analyzed memory formation in GSK-3β heterozygote, knockout mice. Results indicate that these mice show impaired memory reconsolidation. It would seem that activation of GSK-3β is required for memory maintenance, with a higher requirement as animals age, and the volume of memory increases. This in turn causes exaggerated activation of GSK-3β, leading to memory problems, and the formation of NFTs.

  16. Autonomous formation flying in low earth orbit

    NARCIS (Netherlands)

    D'Amico, S.


    Formation flying is commonly identified as the collective usage of two or more cooperative spacecraft to exercise the function of a single monolithic virtual instrument. The distribution of tasks and payloads among fleets of coordinated smaller satellites offers the possibility to overcome the

  17. Ice Formation Delay on Penguin Feathers (United States)

    Alizadehbirjandi, Elaheh; Tavakoli-Dastjerdi, Faryar; St. Leger, Judy; Davis, Stephen H.; Rothstein, Jonathan P.; Kavehpour, H. Pirouz


    Antarctic penguins reside in a harsh environment where air temperature may reach -40 °C with wind speed of 40 m/s and water temperature remains around -2.2 °C. Penguins are constantly in and out of the water and splashed by waves, yet even in sub-freezing conditions, the formation of macroscopic ice is not observed on their feathers. Bird feathers are naturally hydrophobic; however, penguins have an additional hydrophobic coating on their feathers to reinforce their non-wetting properties. This coating consists of preen oil which is applied to the feathers from the gland near the base of the tail. The combination of the feather's hydrophobicity and surface texture is known to increase the contact angle of water drops on penguin feathers to over 140 ° and classify them as superhydrophobic. We here develop an in-depth analysis of ice formation mechanism on superhydrophobic surfaces through careful experimentations and development of a theory to address how ice formation is delayed on these surfaces. Furthermore, we investigate the anti-icing properties of warm and cold weather penguins with and without preen oil to further design a surface minimizing the frost formation which is of practical interest especially in aircraft industry.

  18. Thermal formation of corundum from aluminium hydroxides ...

    Indian Academy of Sciences (India)

    Aluminium hydroxides have been precipitated from various aluminium salts and the differences in their thermal behaviour have been investigated. Pseudoboehmite derived from the nitrate, sulfate and chloride all form -Al2O3 at ∼ 400°C but the formation of -Al2O3 at 1200°C occurs more readily in the material derived ...

  19. Modelling trihalomethanes formation in water supply systems. (United States)

    Di Cristo, Cristiana; Esposito, Giovanni; Leopardi, Angelo


    Chlorination is the most widely used method for disinfection of drinking water, but there are concerns about the formation of by-products, such as trihalomethanes (THMs), since the chronic exposure to them may pose risks to human health. For these reasons regulations fix maximum acceptable THMs levels throughout distribution networks, so it is very important to be able to correctly reproduce their formation. In the literature many models for predicting THMs formation have been developed, both based on empirical relationships and on kinetics involved during chlorine reactions. In this work the use of some of these models and their reliability in real situations is investigated through the application to the Aurunci-Valcanneto Water Supply System in Southern Lazio (Italy). The comparison of the performances of 18 selected literature empirical models furnishes interesting observations, indicating that the formula, developed using field data, results in being more suitable for reproducing THMs formation for the presented case study. Other considerations are also offered from the comparison with the results obtained using a simple first order kinetic model, calibrated using measured data.

  20. Mucocele formation after frontal sinus obliteration

    NARCIS (Netherlands)

    Hansen, F. S.; van der Poel, N. A.; Freling, N. J. M.; Fokkens, W. J.


    A possible complication of frontal sinus obliteration with fat is the formation of mucoceles. We studied the prevalence of mucoceles as well as and the need for revision surgery. Retrospective case review of forty consecutive patients undergoing frontal sinus obliteration from September 1995 to

  1. Amyloid plaque formation precedes dendritic spine loss. (United States)

    Bittner, Tobias; Burgold, Steffen; Dorostkar, Mario M; Fuhrmann, Martin; Wegenast-Braun, Bettina M; Schmidt, Boris; Kretzschmar, Hans; Herms, Jochen


    Amyloid-beta plaque deposition represents a major neuropathological hallmark of Alzheimer's disease. While numerous studies have described dendritic spine loss in proximity to plaques, much less is known about the kinetics of these processes. In particular, the question as to whether synapse loss precedes or follows plaque formation remains unanswered. To address this question, and to learn more about the underlying kinetics, we simultaneously imaged amyloid plaque deposition and dendritic spine loss by applying two-photon in vivo microscopy through a cranial window in double transgenic APPPS1 mice. As a result, we first observed that the rate of dendritic spine loss in proximity to plaques is the same in both young and aged animals. However, plaque size only increased significantly in the young cohort, indicating that spine loss persists even many months after initial plaque appearance. Tracking the fate of individual spines revealed that net spine loss is caused by increased spine elimination, with the rate of spine formation remaining constant. Imaging of dendritic spines before and during plaque formation demonstrated that spine loss around plaques commences at least 4 weeks after initial plaque formation. In conclusion, spine loss occurs, shortly but with a significant time delay, after the birth of new plaques, and persists in the vicinity of amyloid plaques over many months. These findings hence give further hope to the possibility that there is a therapeutic window between initial amyloid plaque deposition and the onset of structural damage at spines.

  2. Local induction of inflammation affects bone formation

    NARCIS (Netherlands)

    Croes, M.|info:eu-repo/dai/nl/413641902; Kruyt, M. C.|info:eu-repo/dai/nl/258751606; Loozen, L.; Kragten, A. H M; Yuan, H.; Dhert, W. J.; Öner, F. C.|info:eu-repo/dai/nl/188615326; Alblas, Jacqueline|info:eu-repo/dai/nl/147759234


    To explore the influence of inflammatory processes on bone formation, we applied a new in vivo screening model. Confined biological pockets were first created in rabbits as a response to implanted bone cement discs. These biomembrane pockets were subsequently used to study the effects of


    NARCIS (Netherlands)


    Resistivity measurements on liquid Rb-In and Cs-In alloys provide evidence for compound formation at a composition of 50% In. The discussion is based on existing electronic structure calculations for solid alloys of Li and Na with Ga, In and Tl. Several possible models for describing liquid


    African Journals Online (AJOL)

    On the basis of the experimental findings, a plausible mechanism for the formation of soluble form of Prussian blue (KFe{Fe(CN)6}x H2O) and rate law are proposed. The activation parameters for the title reaction are estimated. A relatively low energy of activation (23 kJ mol-1) and high negative entropy of activation (-231 ...

  5. On the formation of current ripples

    DEFF Research Database (Denmark)

    Bartholdy, Jesper; Ernstsen, Verner Brandbyge; Flemming, Burg W.


    For grain sizes finer than coarse sand, the first flow-transverse bedforms to develop are current ripples. Although numerous studies have analysed different aspects of bedform morphodynamics, to date no comprehensive physical explanation for the formation of ripples has been given. We offer...

  6. Study of star formation in NGC 1084 (United States)

    Ramya, S.; Sahu, D. K.; Prabhu, T. P.


    We present UBVRI broad-band, Hα narrow-band photometry of the star-forming complexes in the infrared bright galaxy NGC 1084. Results of medium-resolution spectroscopy of some of the brighter complexes are also discussed. Spectroscopic data are used to better estimate the internal reddening within the galaxy which is found to be highly variable and to calculate metallicity which is close to the solar value. A diagnostic diagram identifies the shocked regions within this galaxy. The narrow-band Hα flux and its equivalent width are used to determine the star formation rates of the complexes and the distribution of ages. Star formation rates for a few of the complexes are found to be as high as 0.5Msolar yr-1. The star-forming complexes lie in the age range 3 to 6.5 Myr. A U - B versus V - I colour-colour mixed population model created using the Starburst99 model colours is used to estimate the ages of the stellar populations present within these regions. Using this technique, it is found that the star formation in NGC 1084 has taken place in a series of short bursts over the last 40 Myr or so. It is proposed that the likely trigger for enhanced star formation is merger with a gas-rich dwarf galaxy.

  7. Mark formation modeling in optical rewritable recording

    NARCIS (Netherlands)

    Brusche, J.H.; Segal, A.; Vuik, C.; Urbach, H.P.


    In optical rewritable recording media, such as the Blu-ray Disc, amorphous marks are formed on a crystalline background of a phase-change layer, by means of short, high power laser pulses. In order to improve this data storage concept, it is of great importance to understand the mark formation

  8. Epigenetic Regulation of Memory Formation and Maintenance (United States)

    Zovkic, Iva B.; Guzman-Karlsson, Mikael C.; Sweatt, J. David


    Understanding the cellular and molecular mechanisms underlying the formation and maintenance of memories is a central goal of the neuroscience community. It is well regarded that an organism's ability to lastingly adapt its behavior in response to a transient environmental stimulus relies on the central nervous system's capability for structural…

  9. The Star Formation History of M32

    NARCIS (Netherlands)

    Monachesi, Antonela; Trager, Scott C.; Lauer, Tod R.; Hidalgo, Sebastián L.; Freedman, Wendy; Dressler, Alan; Grillmair, Carl; Mighell, Kenneth J.

    We use deep Hubble Space Telescope Advanced Camera for Surveys/High Resolution Channel observations of a field within M32 (F1) and an M31 background field (F2) to determine the star formation history (SFH) of M32 from its resolved stellar population. We find that 2-5 Gyr old stars contribute ~40% ±

  10. Network formation mechanisms in conjugated microporous polymers


    Laybourn, Andrea; Dawson, Robert; Clowes, Rob; Hasell, Tom; Cooper, Andrew I.; Khimyak, Yaroslav Z.; Adams, Dave J.


    We discuss in detail the mechanism of formation of a highly microporous polymer, CMP-1, formed mainly via Sonogashira–Hagihara coupling. We demonstrate how the microporosity evolves with time, and discuss the importance of alkyne homo-coupling on the microporosity.

  11. MyPlate, Children, and Formative Evaluation

    Directory of Open Access Journals (Sweden)

    Nina Roofe


    Full Text Available Beginning at the critical preschool level, preventing childhood obesity is a multifaceted challenge with health, economic, ethical, and social implications. In particular, increasing emphasis will be placed upon educating children and their caregivers about the USDA’s MyPlate model of good nutrition. To date, evidence-based efforts to teach preschool children nutrition facts and appropriate behaviors are limited, and developers of evidence-based practices do not appear to use formative-evaluation to an adequate extent. Crucial among these evaluations is assessing what the preschool child already knows about the MyPlate components (fruits, vegetables, grains, protein, and dairy. University researchers along with graduate students in nutrition and psychology conducted a review of current research regarding the use of MyPlate in early childhood education settings. Minimal empirical studies were found, indicating a need to expand the literature in the areas of MyPlate, early childhood nutrition education, and formative evaluation. In the current article, authors present the systematic review process of the scant knowledge that exists regarding formative evaluation research to document what preschool-age children already know about nutrition, suggest ways that this research base might be expanded, and advocate for the increased use of formative evaluation in both research and curriculum development.


    Directory of Open Access Journals (Sweden)

    Shavitri Cecillia Harsono


    Full Text Available Word formation is creation of new words, which sometimes changes a word’s meaning. Words can be formed from multi word phrases as well. In many cases vocabularies in language are formed from combination of words (Haspelmath 2010: 102. Word formation does not only involve changing physical form of the word itself, but also changing the meaning of said word. There are also instances where the physical form retain its original form while the meaning changes. The phenomenon is called semantic change (Stockwell-Minkova 2001:149. In this thesis the research proposed that the said phenomenon occur in virtual environment, such as in MMORPG. Multiplayer online games that feature fantasy setting virtual environment. For the purpose of this research, Dragon Nest South East Asia server was chosen as data source. The samples are taken from players perusing [World] communication channel. The result of the data analysis has shown that the phenomenon of word formation could occur in a virtual environment of MMORPG, specifcally in Dragon Nest SEA. There are two word formation processes found: processes that involve physical changes and processes that do not involve physical changes but rather innate meaning. It is done by both processing daily language vocabulary both physically and changing its innate meaning to create new words that suits the said virtual environment context. This fnding may influence future research on a fresh perspective and untilled feld.

  13. Dwarf galaxies : Important clues to galaxy formation

    NARCIS (Netherlands)

    Tolstoy, E


    The smallest dwarf galaxies are the most straight forward objects in which to study star formation processes on a galactic scale. They are typically single cell star forming entities, and as small potentials in orbit around a much larger one they are unlikely to accrete much (if any) extraneous

  14. Galaxies interactions and induced star formation

    CERN Document Server

    Kennicutt Jr, Robert C; Barnes, JE


    The papers that make up this volume present a comprehensive review of the field of galaxy interaction. Galaxies are dynamic forces that evolve, interact, merge, blaze and reshape. This book offers a historical perspective and studies such topics as induced star formation.

  15. The Formation of an Ecological Consciousness (United States)

    Biriukova, N. A.


    In this article, the author discusses how realization of the ideas of humanistic pedagogy and psychology serves as the foundation for the development of value attitudes toward nature and the surrounding world, for the formation of a new type of ecological consciousness. It is obvious that the interaction between human civilization and the earth's…

  16. Surfactant effects on SF6 hydrate formation. (United States)

    Lee, Bo Ram; Lee, Ju Dong; Lee, Hyun Ju; Ryu, Young Bok; Lee, Man Sig; Kim, Young Seok; Englezos, Peter; Kim, Myung Hyun; Kim, Yang Do


    Sulfur hexafluoride (SF(6)) has been widely used in a variety of industrial processes, but it is one of the most potent greenhouse gases. For this reason, it is necessary to separate or collect it from waste gas streams. One separation method is through hydrate crystal formation. In this study, SF(6) hydrate was formed in aqueous surfactant solutions of 0.00, 0.01, 0.05, 0.15 and 0.20 wt% to investigate the effects of surfactants on the hydrate formation rates. Three surfactants, Tween 20 (Tween), sodium dodecyl sulfate (SDS) and linear alkyl benzene sulfonate (LABS), were tested in a semi-batch stirred vessel at the constant temperature and pressures of 276.2 K and 0.78 MPa, respectively. All surfactants showed kinetic promoter behavior for SF(6) hydrate formation. It was also found that SF(6) hydrate formation proceeded in two stages with the second stage being the most rapid. In situ Raman spectroscopy analysis revealed that the increased gas consumption rate with the addition of surfactant was possibly due to the increased gas filling rate in the hydrate cavity.

  17. Granuloma formation in ANCA-associated vasculitides

    NARCIS (Netherlands)

    Lamprecht, Peter; Wieczorek, Stefan; Epplen, Jorg T.; Ambrosch, Petra; Kallenberg, Cees G. M.

    Granuloma formation is a key pathologic finding in two of the anti-neutrophil cytoplasmic autoantibody (ANCA)-associated vasculitides: Wegener's granulomatosis (WG) and Churg-Strauss syndrome (CSS). So far, no animal models have been established convincingly reproducing both vasculitic and

  18. Triggered star formation and its consequences (United States)

    Li, Shule; Frank, Adam; Blackman, Eric G.


    Star formation can be triggered by compression from wind or supernova-driven shock waves that sweep over molecular clouds. Because these shocks will likely contain processed elements, triggered star formation has been proposed as an explanation for short-lived radioactive isotopes (SLRIs) in the Solar system. Previous studies have tracked the triggering event to the earliest phases of collapse and have focused on the shock properties required for both successful star formation and mixing of SLRIs. In this paper, we use adaptive mesh refinement simulation methods, including sink particles, to simulate the full collapse and subsequent evolution of a stable Bonnor- Ebert sphere subjected to a shock and post-shock wind. We track the flow of the cloud material after a star (a sink particle) has formed. For non-rotating clouds, we find robust triggered collapse and little bound circumstellar material remaining around the post-shock collapsed core. When we add initial cloud rotation, we observe the formation of discs around the collapsed core which then interact with the post-shock flow. Our results indicate that these circumstellar discs are massive enough to form planets and are long lived, in spite of the ablation driven by post-shock-flow ram pressure. As a function of the initial conditions, we also track the time evolution of the accretion rates and particle mixing between the ambient wind and cloud material. The latter is maximized for cases of highest Mach number.

  19. Palladium-Catalyzed Asymmetric Quaternary Stereocenter Formation

    NARCIS (Netherlands)

    Gottumukkala, Aditya L.; Matcha, Kiran; Lutz, Martin; de Vries, Johannes G.; Minnaard, Adriaan J.


    An efficient palladium catalyst is presented for the formation of benzylic quaternary stereocenters by conjugate addition of arylboronic acids to a variety of beta,beta-disubstituted carbocyclic, heterocyclic, and acyclic enones. The catalyst is readily prepared from PdCl2, PhBOX, and AgSbF6, and

  20. Palladium-catalyzed asymmetric quaternary stereocenter formation

    NARCIS (Netherlands)

    Gottumukkala, A.L.; Matcha, K.; Lutz, M.|info:eu-repo/dai/nl/304828971; de Vries, J.G.; Minnaard, A.J.


    An efficient palladium catalyst is presented for the formation of benzylic quaternary stereocenters by conjugate addition of arylboronic acids to a variety of β,β-disubstituted carbocyclic, heterocyclic, and acyclic enones. The catalyst is readily prepared from PdCl2, PhBOX, and AgSbF6, and provides

  1. Continuous formation of liturgy through social cognition

    Directory of Open Access Journals (Sweden)

    Ferdi Kruger


    Full Text Available This article researches two focal points, namely liturgical formation and the influence that social cognition has on liturgical formation. Within a South African context it is evident that Western liturgical traditions encounter African traditions and vice versa. This encounter is challenging because it creates new questions. The process of enculturation is prominent in recent research. The article refers to the process of social cognition as the manner in which people observe each other and try to make sense of other cultures and the people of those cultures. People’s cognition can be wrong, leading to distortions. The main research question for this investigation emanates from this possibility, namely: How does social cognition influence the process of liturgical formation? The authors first of all offer a descriptive– empirical vantage point to investigate this matter. Two local congregations were visited. The authors reflect on their own cognition, but also examine the cognition of the leaders through interviews. Based on the findings of this endeavour, normative perspectives are formulated from Acts 17:16–35 to highlight the role of cognition in liturgical formation. Throughout, the article includes consideration of the hermeneutic interaction between the various elements of this research and provides hermeneutic guidelines.

  2. Essays on habit formation and inflation hedging

    NARCIS (Netherlands)

    Zhou, Y.


    The thesis consists of four chapters. Chapter 1 reviews recent contributions on habit formation in the literature and investigates its implications for investors. Chapter 2 revisits the “Floor-Leverage” rule for investors with ratchet consumption preference proposed by Scott and Watson (2011). It

  3. Habit formation, surplus consumption and return predictability

    DEFF Research Database (Denmark)

    Engsted, Tom; Hyde, Stuart; Vinther Møller, Stig


    On an international post World War II dataset, we use an iterated GMM procedure to estimate and test the Campbell and Cochrane (1999, By force of habit: a consumption-based explanation of aggregate stock market behavior. Journal of Political Economy 107, 205–251.) habit formation model with a time...

  4. Formation, isomerization, and derivatization of keggin tungstoaluminates. (United States)

    Jennifer J. Cowan; Alan J. Bailey; Robert A. Heintz; Bao T. Do; Kenneth I. Hardcastle; Craig L. Hill; Ira A. Weinstock


    Trends in the stability of ¥á and ©¬-Keggin heteropolytungstates of the second-row main-group heteroatoms Al(III), Si(IV), and P(V) are elaborated by data that establish the roles of kinetic and thermodynamic control in the formation and isomerization of Keggin tungstoaluminates. Slow, room-temperature co-...

  5. Online Formative Assessments with Social Network Awareness (United States)

    Lin, Jian-Wei; Lai, Yuan-Cheng


    Social network awareness (SNA) has been used extensively as one of the strategies to increase knowledge sharing and collaboration opportunities. However, most SNA studies either focus on being aware of peer's knowledge context or on social context. This work proposes online formative assessments with SNA, trying to address the problems of online…

  6. Field observations of nearshore bar formation

    DEFF Research Database (Denmark)

    Aagaard, Troels; Kroon, Aart; Greenwood, Brian


      The formation of an inner nearshore bar was observed during a high-energy event at the sandy beach of Vejers, Denmark. The bar accreted in situ during surf zone conditions and the growth of the bar was associated with the development of a trough landward of the bar. Measurements of hydrodynamics...

  7. The Lecture-Discussion Format Revisited. (United States)

    Bowman, James S.


    A new look at the standard lecture/discussion format for the large introductory college course is offered, along with suggestions to upgrade its quality. Foci for improvement include organization of lecture material, teacher-student interaction, lecture delivery, feedback, discussion group supervision, and course evaluation. (JMD)

  8. Formation of Aqueous Suspensions of Fullerenes (United States)

    Colloidal suspensions of C60, C70 and a derivative of C60, PCBM ([6,6]-Phenyl C61-butyric acid methyl ester) were produced by extended mixing in water. We examined the contribution of background solution chemistry (pH, ionic strength) on the formation kinetics of colloidal suspe...

  9. Features the internet - formations of students


    Romanova, Inna Sergeevna


    Purpose: research of the attitude of students 2 the Internet - to formation. Metethodology: interrogation and questioning of students, statistical processing of the RCVed information. Results: recommendations on application of the new form of training. Practical implications: the organization of educational process.

  10. Cooperative Hydrogen Bonding in Amyloid Formation.

    Energy Technology Data Exchange (ETDEWEB)

    Tsemekhman, Kiril L.; Goldschmidt, Lukasz; Eisenberg, Dvaid; Baker, David


    The research described in this product was performed in part in the Environmental Molecular Sciences Laboratory, a national scientific user facility sponsored by the Department of Energy's Office of Biological and Environmental Research and located at Pacific Northwest National Laboratory. Amyloid diseases, including Alzheimer's and prion diseases, are each associated with unbranched protein fibrils. Each fibril is made of a particular protein, yet they share common properties. One such property is nucleation-dependent fibril growth. Monomers of amyloid-forming proteins can remain in dissolved form for long periods, before rapidly assembly into fibrils. The lag before growth has been attributed to slow kinetics of formation of a nucleus, on which other molecules can deposit to form the fibril. We have explored the energetics of fibril formation, based on the known molecular structure of a fibril-forming peptide from the yeast prion, Sup35, using both classical and quantum (density functional theory) methods. We find that the energetics of fibril formation for the first three layers are cooperative using both methods. This cooperativity is consistent with the observation that formation of amyloid fibrils involves slow nucleation and faster growth.

  11. Global models of planet formation and evolution (United States)

    Mordasini, C.; Mollière, P.; Dittkrist, K.-M.; Jin, S.; Alibert, Y.


    Despite the strong increase in observational data on extrasolar planets, the processes that led to the formation of these planets are still not well understood. However, thanks to the high number of extrasolar planets that have been discovered, it is now possible to look at the planets as a population that puts statistical constraints on theoretical formation models. A method that uses these constraints is planetary population synthesis where synthetic planetary populations are generated and compared to the actual population. The key element of the population synthesis method is a global model of planet formation and evolution. These models directly predict observable planetary properties based on properties of the natal protoplanetary disc, linking two important classes of astrophysical objects. To do so, global models build on the simplified results of many specialized models that address one specific physical mechanism. We thoroughly review the physics of the sub-models included in global formation models. The sub-models can be classified as models describing the protoplanetary disc (of gas and solids), those that describe one (proto)planet (its solid core, gaseous envelope and atmosphere), and finally those that describe the interactions (orbital migration and N-body interaction). We compare the approaches taken in different global models, discuss the links between specialized and global models, and identify physical processes that require improved descriptions in future work. We then shortly address important results of planetary population synthesis like the planetary mass function or the mass-radius relationship. With these statistical results, the global effects of physical mechanisms occurring during planet formation and evolution become apparent, and specialized models describing them can be put to the observational test. Owing to their nature as meta models, global models depend on the results of specialized models, and therefore on the development of


    Energy Technology Data Exchange (ETDEWEB)

    Leschine, Susan


    This project addressed four major areas of investigation: i) characterization of formation of Cellulomonas uda biofilms on cellulose; ii) characterization of Clostridium phytofermentans biofilm development; colonization of cellulose and its regulation; iii) characterization of Thermobifida fusca biofilm development; colonization of cellulose and its regulation; and iii) description of the architecture of mature C. uda, C. phytofermentans, and T. fusca biofilms. This research is aimed at advancing understanding of biofilm formation and other complex processes involved in the degradation of the abundant cellulosic biomass, and the biology of the microbes involved. Information obtained from these studies is invaluable in the development of practical applications, such as the single-step bioconversion of cellulose-containing residues to fuels and other bioproducts. Our results have clearly shown that cellulose-decomposing microbes rapidly colonize cellulose and form complex structures typical of biofilms. Furthermore, our observations suggest that, as cells multiply on nutritive surfaces during biofilms formation, dramatic cell morphological changes occur. We speculated that morphological changes, which involve a transition from rod-shaped cells to more rounded forms, might be more apparent in a filamentous microbe. In order to test this hypothesis, we included in our research a study of biofilm formation by T. fusca, a thermophilic cellulolytic actinomycete commonly found in compost. The cellulase system of T. fusca has been extensively detailed through the work of David Wilson and colleagues at Cornell, and also, genome sequence of a T. fusca strain has been determine by the DOE Joint Genome Institute. Thus, T. fusca is an excellent subject for studies of biofilm development and its potential impacts on cellulose degradation. We also completed a study of the chitinase system of C. uda. This work provided essential background information for understanding how C. uda

  13. Protocol for Communication Networking for Formation Flying (United States)

    Jennings, Esther; Okino, Clayton; Gao, Jay; Clare, Loren


    An application-layer protocol and a network architecture have been proposed for data communications among multiple autonomous spacecraft that are required to fly in a precise formation in order to perform scientific observations. The protocol could also be applied to other autonomous vehicles operating in formation, including robotic aircraft, robotic land vehicles, and robotic underwater vehicles. A group of spacecraft or other vehicles to which the protocol applies could be characterized as a precision-formation- flying (PFF) network, and each vehicle could be characterized as a node in the PFF network. In order to support precise formation flying, it would be necessary to establish a corresponding communication network, through which the vehicles could exchange position and orientation data and formation-control commands. The communication network must enable communication during early phases of a mission, when little positional knowledge is available. Particularly during early mission phases, the distances among vehicles may be so large that communication could be achieved only by relaying across multiple links. The large distances and need for omnidirectional coverage would limit communication links to operation at low bandwidth during these mission phases. Once the vehicles were in formation and distances were shorter, the communication network would be required to provide high-bandwidth, low-jitter service to support tight formation-control loops. The proposed protocol and architecture, intended to satisfy the aforementioned and other requirements, are based on a standard layered-reference-model concept. The proposed application protocol would be used in conjunction with conventional network, data-link, and physical-layer protocols. The proposed protocol includes the ubiquitous Institute of Electrical and Electronics Engineers (IEEE) 802.11 medium access control (MAC) protocol to be used in the datalink layer. In addition to its widespread and proven use in

  14. Fracture formation post impact on Enceladus? (United States)

    Craft, Kathleen; Roberts, James


    Saturn’s small icy moon Enceladus was observed by the Cassini mission to have jets of ice and vapor emanating from its southern polar terrain (SPT), creating a plume. The fact that the activity is only observed in one region has not been well explained. Hypotheses include a regional sea beneath the SPT or a global ocean that is thicker beneath the SPT, which feeds a group of fractures observed there called the tiger stripes. As Enceladus orbits Saturn, stresses acting on the moon may open and close the fractures enabling interior volatiles to escape and form the plume. Here we investigate how these fractures could have formed and the activity begun. We propose that an impact could have either punctured through or caused substantial melt and fracturing in an ice shell connecting to a liquid layer below. Our goal is to determine whether a formation of fractures resembling the tiger stripes could emerge post-impact.Previous work by Roberts and Stickle (LPSC 2017, #1955) modeled an impact into an ice shell over an ocean and calculated penetration depth and melt temperatures and volumes through the shell thickness. Fracturing would occur during and after the impact, the crater would collapse, water would begin to refreeze and subsequent fluid exchange would occur. Working forward from a point after impact and as the ice shell begins refreezing, we performed finite element modeling to simulate the probable formation of fractures based on the resulting stress regime. Here we explore fracture formation for shells ranging from 1 km to 5 km thick (consistent with gravity and libration studies), to explore formation as the shell cools and thickens through time. We emplaced several fractures, penetrating either entirely or partially across the base to surface. Fracture interactions, tidal stress forcing with orbital true anomalies and ocean water pressurization are considered free parameters in the model. We present results for a number of parameter value combinations and

  15. Photochemical Formation of Sulfur-Containing Aerosols (United States)

    Kroll, Jay A.; Vaida, Veronica


    In order to understand planetary climate systems, modeling the properties of atmospheric aerosols is vital. Aerosol formation plays an important role in planetary climates and is tied to feedback loops that can either warm or cool a planet. Sulfur compounds are known to play an important role in new particle aerosol formation and have been observed in a number of planetary atmospheres throughout our solar system. Our current understanding of sulfur chemistry explains much of what we observe in Earth's atmosphere; however, several discrepancies arise when comparing observations of the Venusian atmosphere with model predictions. This suggests that there are still problems in our fundamental understanding of sulfur chemistry. This is concerning given recent renewed interest in sulfate injections in the stratosphere for solar radiation management geo-engineering schemes. We investigate the role of sunlight as a potential driver of the formation of sulfur-containing aerosols. I will present recent work investigating the generation of large quantities of aerosol from the irradiation of mixtures of SO_2 with water and organic species, using a solar simulator that mimics the light that is available in the Earth's troposphere and the Venusian middle atmosphere. I will present on recent work done in our lab suggesting the formation of sulfurous acid, H_2SO_3, and describe experimental work that supports this proposed mechanism. Additionally I will present on new work showing the highly reactive nature of electronically excited SO_2 with saturated alkane species. The implications of this photochemically induced sulfur aerosol formation in the atmosphere of Earth and other planetary atmospheres will be discussed.

  16. Observational insights into aerosol formation from isoprene. (United States)

    Worton, David R; Surratt, Jason D; Lafranchi, Brian W; Chan, Arthur W H; Zhao, Yunliang; Weber, Robin J; Park, Jeong-Hoo; Gilman, Jessica B; de Gouw, Joost; Park, Changhyoun; Schade, Gunnar; Beaver, Melinda; Clair, Jason M St; Crounse, John; Wennberg, Paul; Wolfe, Glenn M; Harrold, Sara; Thornton, Joel A; Farmer, Delphine K; Docherty, Kenneth S; Cubison, Michael J; Jimenez, Jose-Luis; Frossard, Amanda A; Russell, Lynn M; Kristensen, Kasper; Glasius, Marianne; Mao, Jingqiu; Ren, Xinrong; Brune, William; Browne, Eleanor C; Pusede, Sally E; Cohen, Ronald C; Seinfeld, John H; Goldstein, Allen H


    Atmospheric photooxidation of isoprene is an important source of secondary organic aerosol (SOA) and there is increasing evidence that anthropogenic oxidant emissions can enhance this SOA formation. In this work, we use ambient observations of organosulfates formed from isoprene epoxydiols (IEPOX) and methacrylic acid epoxide (MAE) and a broad suite of chemical measurements to investigate the relative importance of nitrogen oxide (NO/NO2) and hydroperoxyl (HO2) SOA formation pathways from isoprene at a forested site in California. In contrast to IEPOX, the calculated production rate of MAE was observed to be independent of temperature. This is the result of the very fast thermolysis of MPAN at high temperatures that affects the distribution of the MPAN reservoir (MPAN / MPA radical) reducing the fraction that can react with OH to form MAE and subsequently SOA (F(MAE formation)). The strong temperature dependence of F(MAE formation) helps to explain our observations of similar concentrations of IEPOX-derived organosulfates (IEPOX-OS; ~1 ng m(-3)) and MAE-derived organosulfates (MAE-OS; ~1 ng m(-3)) under cooler conditions (lower isoprene concentrations) and much higher IEPOX-OS (~20 ng m(-3)) relative to MAE-OS (water content when aerosol pH is constant. However, the higher fraction of MAE ring opening products does not compensate for the lower MAE production under warmer conditions (higher isoprene concentrations) resulting in lower formation of MAE-derived products relative to IEPOX at the surface. In regions of high NOx, high isoprene emissions and strong vertical mixing the slower MPAN thermolysis rate aloft could increase the fraction of MPAN that forms MAE resulting in a vertically varying isoprene SOA source.

  17. A calorimetric study of carbamate formation

    Energy Technology Data Exchange (ETDEWEB)

    McCann, N.; Maeder, M.; Hasse, H. [Technical University of Kaiserslautern, Kaiserslautern (Germany)


    Post combustion capture of CO{sub 2} (PCC) is currently one of the leading technologies for the reduction of greenhouse gas emissions from power plants. The most common PCC process is based on the absorption of CO{sub 2} into aqueous amine solutions. CO{sub 2} absorption involves several parallel reactions including hydration of CO{sub 2}; deprotonation of carbonic acid; protonation of the amine; and formation of carbamate. The extent to which each reaction proceeds is dependent on the conditions of absorption (or desorption), as well as the associated equilibrium constants. In this contribution, we use calorimetry and advanced model-based data analysis methods for the unravelling of the thermo-chemistry relevant to PCC and specifically directly determine the reaction enthalpy for carbamate formation. The reaction enthalpies of carbamate formation and amine protonation were measured in dilute aqueous solution at 298 K using isothermal titration calorimetry (ITC) for monoethanolamine (MEA), diethanolamine (DEA), and ammonia (NH{sub 3}). The enthalpy of protonation was also measured for carbonate and bicarbonate. The re-determined protonation constants of the carbonate species and the three amines are in excellent agreement with previously reported results. No measured enthalpies of carbamate formation have been reported previously. For the carbamate formation reaction HCO{sub 3}{sup -} + R'RNH {longleftrightarrow}R'RNCO{sub 2}{sup -}, the following reaction enthalpies were determined: MEA, -29.7 {+-} 0.1 kJ/mol; DEA, -23.7 {+-} 0.9 kJ/mol, and NH{sub 3}, -27.6 {+-} 0.9 kJ/mol. The results are in good agreement with, but much more precise and robust than estimated values reported in the literature.

  18. Blood drop patterns: Formation and applications. (United States)

    Chen, Ruoyang; Zhang, Liyuan; Zang, Duyang; Shen, Wei


    The drying of a drop of blood or plasma on a solid substrate leads to the formation of interesting and complex patterns. Inter- and intra-cellular and macromolecular interactions in the drying plasma or blood drop are responsible for the final morphologies of the dried patterns. Changes in these cellular and macromolecular components in blood caused by diseases have been suspected to cause changes in the dried drop patterns of plasma and whole blood, which could be used as simple diagnostic tools to identify the health of humans and livestock. However, complex physicochemical driving forces involved in the pattern formation are not fully understood. This review focuses on the scientific development in microscopic observations and pattern interpretation of dried plasma and whole blood samples, as well as the diagnostic applications of pattern analysis. Dried drop patterns of plasma consist of intricate visible cracks in the outer region and fine structures in the central region, which are mainly influenced by the presence and concentration of inorganic salts and proteins during drying. The shrinkage of macromolecular gel and its adhesion to the substrate surface have been thought to be responsible for the formation of the cracks. Dried drop patterns of whole blood have three characteristic zones; their formation as functions of drying time has been reported in the literature. Some research works have applied engineering treatment to the evaporation process of whole blood samples. The sensitivities of the resultant patterns to the relative humidity of the environment, the wettability of the substrates, and the size of the drop have been reported. These research works shed light on the mechanisms of spreading, evaporation, gelation, and crack formation of the blood drops on solid substrates, as well as on the potential applications of dried drop patterns of plasma and whole blood in diagnosis. Crown Copyright © 2016. Published by Elsevier B.V. All rights reserved.


    Directory of Open Access Journals (Sweden)



    Full Text Available Background: Vocal fold polyp is one of the most common causes for hoarseness. Many different etiological factors contribute to vocal fold polyp formation. The aim of the study was to find out whether the etiological factors for polyp formation have changed in the last 30 years.Methods: Eighty-one patients with unilateral vocal fold polyp were included in the study. A control group was composed of 50 volunteers without voice problems who matched the patients by age and gender. The data about etiological factors and the findings of phoniatric examination were obtained from the patients' medical documentation and from the questionnaires for the control group. The incidence of etiological factors was compared between the two groups. The program SPSS, Version 18 was used for statistical analysis.Results: The most frequent etiological factors were occupational voice load, GER, allergy and smoking. In 79% of patients 2 – 6 contemporary acting risk factors were found. Occupational voice load (p=0,018 and GER (p=0,004 were significantly more frequent in the patients than in the controls. The other factors did not significantly influence the polyp formation.Conclusions: There are several factors involved simultaneously in the formation of vocal fold polyps both nowadays and 30 years ago. Some of the most common factors remain the same (voice load, smoking, others are new (GER, allergy, which is probably due to the different lifestyle and working conditions than 30 years ago. Occupational voice load and GER were significantly more frequently present in the patients with polyp than in the control group. Regarding the given results it is important to instruct workers with professional vocal load about etiological factors for vocal fold polyp formation.

  20. Power systems utilizing the heat of produced formation fluid (United States)

    Lambirth, Gene Richard [Houston, TX


    Systems, methods, and heaters for treating a subsurface formation are described herein. At least one method includes treating a hydrocarbon containing formation. The method may include providing heat to the formation; producing heated fluid from the formation; and generating electricity from at least a portion of the heated fluid using a Kalina cycle.

  1. Seminary Formation: A Case Study from the Pontifical Beda College, Rome (United States)

    Strange, Roderick


    This case study account reviews issues related to seminary formation and education at the Beda College, Rome, including Fundamentals of Formation, Community Life, Organizing Formation, Intellectual Formation, Spiritual Formation, Pastoral Formation, and the challenges arising in these fields.

  2. Laboratory Studies Of Circumstellar Carbonaceous Grain Formation (United States)

    Contreras, Cesar; Sciamma-O'Brien, Ella; Salama, Farid


    The study of the formation processes of dust is essential to understand the budget of extraterrestrial organic molecules. Although dust with all its components plays an important role in the evolution of interstellar (IS) chemistry and in the formation of organic molecules, little is known on the formation processes of carbonaceous dust. We report the progress that was recently achieved in this domain using NASA Ames’ COSmIC facility (Contreras & Salama 2013, ApJS, 208, 6). PAHs are important chemical building blocks of IS dust. They are detected in IDPs and in meteoritic samples. Additionally, observational, laboratory, and theoretical studies have shown that PAHs are an important, ubiquitous component of the ISM. The formation of PAHs from smaller molecules has not been extensively studied. Therefore, we have performed laboratory experiments to study the dynamic processes of carbon grain formation, starting from the smallest hydrocarbon molecules into the formation of larger PAH and further into nanograins. Studies of IS dust analogs formed from a variety of PAH and hydrocarbon precursors as well as species that include the atoms O, N, and S, have recently been performed in our laboratory using the COSmIC facility to provide conditions that simulate IS and circumstellar environments. The species formed in the COSmiC chamber through a pulsed discharge nozzle plasma source are detected and characterized with a cavity ringdown spectrometer coupled to a time-of-flight mass spectrometer, thus providing both spectroscopic and ion mass information in-situ. Analysis of solid soot particles was also conducted using scanning electron microscopy at the UCSC/NASA Ames’ MACS facility. The SEM analysis of the deposition of soot from methane and acetylene precursors seeded in argon plasmas provide examples on the types of nanoparticles and micrograins that are produced in these gas mixtures under our experimental conditions. From these measurements, we derive information on

  3. Star Formation Quenching in Quasar Host Galaxies

    Directory of Open Access Journals (Sweden)

    Stefano Carniani


    Full Text Available Galaxy evolution is likely to be shaped by negative feedback from active galactic nuclei (AGN. In the whole range of redshifts and luminosities studied so far, galaxies hosting an AGN frequently show fast and extended outflows consisting in both ionized and molecular gas. Such outflows could potentially quench the start formation within the host galaxy, but a clear evidence of negative feedback in action is still missing. Hereby I will analyse integral-field spectroscopic data for six quasars at z ~ 2.4 obtained with SINFONI in the H- and K-band. All the quasars show [Oiii]λ5007 line detection of fast, extended outflows. Also, the high signal-to-noise SINFONI observations allow the identification of faint narrow Hα emission (FWHM < 500 km/s, which is spatially extended and associated with star formation in the host galaxy. On paper fast outflows are spatially anti-correlated with star-formation powered emission, i.e., star formation is suppressed in the area affected by the outflow. Nonetheless as narrow, spatially-extended Hα emission, indicating star formation rates of at least 50–100 M⊙ yr−1, has been detected, either AGN feedback is not affecting the whole host galaxy, or star formation is completely quenched only by several feedback episodes. On the other hand, a positive feedback scenario, supported by narrow emission in Hα extending along the edges of the outflow cone, suggests that galaxy-wide outflows could also have a twofold role in the evolution of the host galaxy. Finally, I will present CO(3-2 ALMA data for three out of the six QSOs observed with SINFONI. Flux maps obtained for the CO(3-2 transition suggest that molecular gas within the host galaxy is swept away by fast winds. A negative-feedback scenario is supported by the inferred molecular gas mass in all three objects, which is significantly below what observed in non-active main-sequence galaxies at high-z.

  4. ASDF - A Modern Data Format for Seismology (United States)

    Krischer, Lion; Smith, James; Lei, Wenjie; Lefebvre, Matthieu; Ruan, Youyi; Sales de Andrade, Elliot; Podhorszki, Norbert; Bozdag, Ebru; Tromp, Jeroen


    Seismology as a science is driven by observing and understanding data and it is thus vital to make this as easy and accessible as possible. The growing volume of freely available data coupled with ever expanding computational power enables scientists to take on new and bigger problems. This evolution is to some part hindered as existing data formats have not been designed with it in mind. We present ASDF (, the Adaptable Seismic Data Format, a novel, modern, and especially practical data format for all branches of seismology with particular focus on how it is incorporated into seismic full waveform inversion workflows. The format aims to solve five key issues: Efficiency: Fast I/O operations especially in high performance computing environments, especially limiting the total number of files. Data organization: Different types of data are needed for a variety of tasks. This results in ad hoc data organization and formats that are hard to maintain, integrate, reproduce, and exchange. Data exchange: We want to exchange complex and complete data sets. Reproducibility: Oftentimes just not existing but crucial to advance our science. Mining, visualization, and understanding of data: As data volumes grow, more complex, new techniques to query and visualize large datasets are needed. ASDF tackles these by defining a structure on top of HDF5 reusing as many existing standards (QuakeML, StationXML, PROV) as possible. An essential trait of ASDF is that it empowers the construction of completely self-describing data sets including waveform, station, and event data together with non-waveform data and a provenance description of everything. This for example for the first time enables the proper archival and exchange of processed or synthetic waveforms. To aid community adoption we developed mature tools in Python as well as in C and Fortran. Additionally we provide a formal definition of the format, a validation tool, and integration into widely used


    Directory of Open Access Journals (Sweden)

    Myronenko L.G.


    Full Text Available Introduction. Today, it is well established that almost 80% of all infectious diseases are caused by microorganisms that exist in the form of biofilms. Microorganisms in the biofilms acquire signs of increased resistance to antibiotics, disinfectants and other aggressive environmental factors, complicate the course of infectious diseases and play an important role in their chronicity. Formation of biofilms by hospital strains of bacteria poses a serious threat to the practical medicine. Enterococci, foremost Enterococcus faecium and Enterococcus faecalis, are the third most common cause of hospital infections, most of which involve the use of permanent medical equipment. Internal hospital infections gain particular importance in intensive care units and in surgical hospitals, since the formation of biofilms is the cause of severe catheter and fan associated infections, sepsis, pneumonia and endocarditis. It should be noted that ineffective antibiotic therapy of infections, accompanied by the formation of biofilms, also leads to significant economic losses. The aim of the work was to study the effects of gentamicin and gentamicin in combination with a penetrator on the processes of enterococci biofilm formation. Materials and methods. The objects of the study included 3 strains of bacteria genus Enterococcus, obtained from the bacteria museum of the Mechnikov Institute of Microbiology and Immunology National Academy of Medical Sciences of Ukraine: E. faecalis ATCC 29212, E. faecalis ІМІ (Х 49 р, E. faecium ІМІ (Х 80. The biofilms modelling was performed in 4-section polystyrene Petri dishes. To study the influence of compounds on biofilm formation, a photometric method was used. The optical density (OD of eluates from enterococci biofilms, stained with crystal violet, was measured with the SF-56L spectrophotometer at a wavelength of 590 nm. Statistical processing of the obtained data was carried out by means of nonparametric statistical


    Directory of Open Access Journals (Sweden)

    Наталія Сіранчук


    Full Text Available The article describes the efficiency of junior students’ lexical competence development by means of word-formation which is direcedt on enriching passive and active vocabular of the studentsy. The main ways of lexical competence development of junior students by means of word-formation are highlighted in the article. the principal factors that help to learn the lexical items are described. The factors which facilitate the interpretation of derived lexical items are emphasized. Conditions which influence the effectiveness of word-formation analysis in primary school are indicated. Knowledge and students’ skills that are required for development of lexical competence by means of word-formation are selected. The essential feature of experimental technique (the constant attention to the contet and functions of morphemes are specified.

  7. [Management competences in the formation of nurses]. (United States)

    Lourenção, Daniela Campos de Andrade; Benito, Gladys Amélia Véles


    This study had as purpose to identify the insertion of the management competences in the formation of nurses. That was a study of qualitative approach, that used the analysis of content of the pedagogic documents of the course of nursing of an institution of higher education of the South Region of the country. In the thematic documental analysis developed in this study, the evidence of the meaning items corroborated the insert of the managerial competence in the dimensions: knowledge, abilities and attitudes. The study demonstrated that they are contemplated in the nurse formation the managerial competences in the researched course. It important to say that from the analysis of the data emerged the enrolled gaps as contribution of the study.

  8. Predictive modeling of post bioprinting structure formation. (United States)

    McCune, Matthew; Shafiee, Ashkan; Forgacs, Gabor; Kosztin, Ioan


    Cellular particle dynamics (CPD) is an effective computational method to describe the shape evolution and biomechanical relaxation processes in systems composed of micro tissues such as multicellular aggregates. Therefore, CPD is a useful tool to predict the outcome of postprinting structure formation in bioprinting. The predictive power of CPD has been demonstrated for multicellular systems composed of identical volume-conserving spherical and cylindrical bioink units. Experiments and computer simulations were related through an independently developed theoretical formalism based on continuum mechanics. Here we generalize the CPD formalism to (i) include non-identical bioink particles often used in specific bioprinting applications, (ii) describe the more realistic experimental situation in which during the post-printing structure formation via the fusion of spherical bioink units the volume of the system decreases, and (iii) directly connect CPD simulations to the corresponding experiments without the need of the intermediate continuum theory inherently based on simplifying assumptions.

  9. Secondary ledeburite formation during various welding techniques

    Directory of Open Access Journals (Sweden)

    Tonkovič M.P.


    Full Text Available The occurrence and formation sequence of secondary ledeburite in the heat affected zone of chromium ledeburitic tool steel W.Nr. 1.2379 (OCR12 VM after welding with SAW, TIG, microplasma and laser welding techniques is presented in this paper. Special attention was paid on the behaviour of carbides. The occurrence of secondary ledeburite is a result of local enrichment of the austenite matrix with alloying elements, due to partial or complete dissolution of primary/eutectic carbides. The results show that the largest amount of secondary ledeburite is formed during submerged arc welding, followed by TIG and microplasma welding technique. Welding by laser technique, with appropriate technological parameters, could prevent secondary ledeburite formation.

  10. Crack formation and prevention in colloidal drops (United States)

    Kim, Jin Young; Cho, Kun; Ryu, Seul-A.; Kim, So Youn; Weon, Byung Mook


    Crack formation is a frequent result of residual stress release from colloidal films made by the evaporation of colloidal droplets containing nanoparticles. Crack prevention is a significant task in industrial applications such as painting and inkjet printing with colloidal nanoparticles. Here, we illustrate how colloidal drops evaporate and how crack generation is dependent on the particle size and initial volume fraction, through direct visualization of the individual colloids with confocal laser microscopy. To prevent crack formation, we suggest use of a versatile method to control the colloid-polymer interactions by mixing a nonadsorbing polymer with the colloidal suspension, which is known to drive gelation of the particles with short-range attraction. Gelation-driven crack prevention is a feasible and simple method to obtain crack-free, uniform coatings through drying-mediated assembly of colloidal nanoparticles.

  11. Cracks formation during blood drop evaporation (United States)

    Sobac, Benjamin; Brutin, David; Université de Provence Team


    We firstly presented the pattern formation occurring when drops of whole blood desiccate in a recent publication. The phenomena presented evidence to involve lots of physical field such as surface chemistry, haematolology, fluid mechanics, heat transfer, colloids science... All these mechanisms are acting together and produce an axisymetric and reproducible pattern. Dried cellular components are segregated and deposited by a capillary flow. During the evaporation, the system is slowly drying and cracks when stresses are too important leading to the final pattern observed. In this presentation, we will present the mechanisms involved in the formation of crack patterns. The phenomenon presented here with red blood cells as the main colloids involved is very similar to the drying of drop of nanoparticules. We will explain the common point and the differences encountered.

  12. Formation of the Isthmus of Panama (United States)

    O'Dea, Aaron; Aguilera, Orangel; Aubry, Marie-Pierre; Berggren, William A.; Cione, Alberto L.; Coates, Anthony G.; Collins, Laurel S.; Coppard, Simon E.; Cozzuol, Mario A.; de Queiroz, Alan; Duque-Caro, Herman; Eytan, Ron I.; Farris, David W.; Gasparini, German M.; Grosmman, Ethan L.; Jackson, Jeremy B. C.; Johnson, Kenneth G.; Keigwin, Lloyd D.; Knowlton, Nancy; Leigh, Egbert G.; Leonard-Pingel, Jill S.; Lessios, Hailaos A.; Marko, Peter B.; Norris, Richard D.; Rachello-Dolmen, Paola G.; Restrepo-Moreno, Sergio A.; Soibelzon, Leopoldo; Stallard, Robert F.; Todd, Jonathan A.; Vermeiju, Geerat J.; Woodburne, Michael O.


    The formation of the Isthmus of Panama stands as one of the greatest natural events of the Cenozoic, driving profound biotic transformations on land and in the oceans. Some recent studies suggest that the Isthmus formed many millions of years earlier than the widely recognized age of approximately 3 million years ago (Ma), a result that if true would revolutionize our understanding of environmental, ecological, and evolutionary change across the Americas. To bring clarity to the question of when the Isthmus of Panama formed, we provide an exhaustive review and reanalysis of geological, paleontological, and molecular records. These independent lines of evidence converge upon a cohesive narrative of gradually emerging land and constricting seaways, with formation of the Isthmus of Panama sensu stricto around 2.8 Ma. The evidence used to support an older isthmus is inconclusive, and we caution against the uncritical acceptance of an isthmus before the Pliocene.

  13. Multiwavelength Mapping of Galaxy Formation and Evolution

    CERN Document Server

    Renzini, Alvio; ESO Workshop


    The possibilities of astronomical observation have dramatically increased over the last decade. Major satellites, like the Hubble Space Telescope, Chandra and XMM Newton, are complemented by numerous large ground-based observatories, from 8m-10m optical telescopes to sub-mm and radio facilities. As a result, observational astronomy has access to virtually the whole electromagnetic spectrum of galaxies, even at high redshifts. Theoretical models of galaxy formation and cosmological evolution now face a serious challenge to match the plethora of observational data. In October 2003, over 170 astronomers from 15 countries met for a 4-day workshop to extensively illustrate and discuss all major observational projects and ongoing theoretical efforts to model galaxy formation and evolution. This volume contains the complete proceedings of this meeting and is therefore a unique and timely overview of the current state of research in this rapidly evolving field.

  14. Meningococcal Biofilm Formation: Let's Stick Together. (United States)

    Arenas, Jesús; Tommassen, Jan


    Extracellular DNA (eDNA) is an essential constituent of the extracellular matrix of biofilms of many microorganisms. In spite of many studies, it has long remained unclear how exactly eDNA exerts its role in biofilm formation. Here, we describe recent advances that have been made in understanding biofilm formation in the human pathogen Neisseria meningitidis. Several cell-surface-exposed proteins have been identified that bind DNA and other negatively charged polymers, such as heparin, by electrostatic interactions. By virtue of these proteins, eDNA can act as an adhesive that binds the bacteria together. We provide examples that indicate that the mechanism of binding eDNA via DNA/heparin-binding proteins is a conserved feature in biofilms of many different microorganisms, including fungi. Copyright © 2016 Elsevier Ltd. All rights reserved.

  15. Taming contact line instability for pattern formation (United States)

    Deblais, A.; Harich, R.; Colin, A.; Kellay, H.


    Coating surfaces with different fluids is prone to instability producing inhomogeneous films and patterns. The contact line between the coating fluid and the surface to be coated is host to different instabilities, limiting the use of a variety of coating techniques. Here we take advantage of the instability of a receding contact line towards cusp and droplet formation to produce linear patterns of variable spacings. We stabilize the instability of the cusps towards droplet formation by using polymer solutions that inhibit this secondary instability and give rise to long slender cylindrical filaments. We vary the speed of deposition to change the spacing between these filaments. The combination of the two gives rise to linear patterns into which different colloidal particles can be embedded, long DNA molecules can be stretched and particles filtered by size. The technique is therefore suitable to prepare anisotropic structures with variable properties.

  16. Compositional constraints on giant planet formation (United States)

    Owen, Tobias; Encrenaz, Therese


    Using Ockham's razor as a guide, we have tried to find the simplest model for the formation of giant planets that can explain current observations of atmospheric composition. While this "top-down" approach is far from sufficient to define such models, it establishes a set of boundary conditions whose satisfaction is necessary. Using Jupiter as the prototype, we find that a simple model for giant planet formation that begins with a solar nebula of uniform composition and relies on accretion of low temperature icy planetesimals plus collapse of surrounding solar nebula gas supplies that satisfaction. We compare the resulting predictions of elemental abundances and isotope ratios in the atmospheres of the other giants with those from contrasting models and suggest some key measurements to make further progress.

  17. Student's Video Production as Formative Assessment

    CERN Document Server

    Gama, Eduardo


    Learning assessments are subject of discussions that envolve theoretical and practical approaches. To measure learning in physics by high school students, either qualitatively or quantitatively, is a process in which it should be possible to identify not only the concepts and contents students failed to achieve but also the reasons of the failure. We propose that students' video production offers a very effective formative assessment to teachers: as a formative assessment, it produces information that allows the understanding of where and when the learning process succeeded or failed, of identifying, as a subject or as a group, the defficiencies or misunderstandings related to the theme under analysis and their interpretation by students, and it provides also a different kind of assessment, related to some other life skills, like the ability to carry a project to its conclusion and to work cooperatively. In this paper, we describe the use of videos produced by high school students as an assessment resource. T...

  18. A Star Formation/ISM Astronomical Database (United States)

    Molinari, Sergio; Ali, Babar; Good, John; Noriega-Crespo, Alberto


    The Star Formation/ISM Astronomical Database (hereafter SFD) will be a set of on-line services adding value to existing data archives and published journals, along the lines of the very successful NASA/IPAC Extragalactic Database (NED) and SIMBAD projects but with a focus on star formation an the interstellar medium (ISM) within the Milky Way. Unlike NED and SIMBAD, however, the SFD must deal with multi-wavelength measurements of extended regions and cross-correlative relationships between disparate measurements. The SFD will rely heavily on existing databases, primarily adding data content and connectivity between datasets around the world, and custom tailoring of existing tools to provide interfaces (programming API, Web, and JAVA GUI) specific to this application. We consider the SFD as a valuable component in the broader context of a future Virtual Observatory.

  19. Magnetic Model Trap Formation of Volyn (United States)

    Yusypiv, M. V.


    In the article technique of magnetic modelling trap formation of Volyn (north-west Ukraine) is considered. It is investigated magnetic properties of rock trap formation, anomalous magnetic field and connection of these parameters with a technique of maning model. The model of a magnetic field was made. The comparative analysis with others trap formations of the world was carried out. Introduction Study of trap magnetism constantly attracts attention of geoscientists throughout the world and may be determined, on the one hand, by connection of a number of important mineral products (copper-nickel, iron and other ores) with trap formations, and on the other hand, has a great importance for solution of general and vital theoretical problems of geosciences.The works of the last ten years' [1-3] study of trap magnetic parameters make it possible to obtain information of magnetism age, geological structure of the territory. Investigations related to making of trap models are of great interest. Making of Volyn region trap formation magnetic model Model 1. The lower limitation is an upper reflection of a ground M. Magnetization of a basalt layer is changed laterally from 1,0 SI units to 5,5 SI units. The field selected in that way is different from the initial field to 30-40 nTl that can be sufficient for modelling of a regional field. Model 2. The lower limitation of a magnetoactive layer is a lower reflection of the M limit. In this case the changes of a lateral magnetization were received from 1,0 to 4,5 SI units. Conclusion. The approach of a model making proved a true possibility for use of a magnetic field not only for traditional study of a near-surface but also deep zones of the earth's crust in conditions of platform areas, zones of spreading of trap formations. The evaluation of contribution of the upper part of the earth's crust to the anomalous field made it possible to mark a regional component of the field by geotraverse II related to the deep parts of the

  20. Fossil birds of the Kibish Formation. (United States)

    Louchart, Antoine; Haile-Selassie, Y; Vignaud, P; Likius, A; Brunet, M


    The Kibish Formation has yielded a small collection of bird fossils, which are identified here as belonging to five species in four different families: Pelecanidae (pelicans), Anhingidae (darters), Ardeidae (herons) and Phasianidae (gamefowl). Two species of pelicans are identified: Pelecanus cf. P. onocrotalus, and P. aff. P. rufescens. The darter is referrable to Anhinga melanogaster. The heron is identifiable as Ardea sp., and the gamefowl as Numidinae indet. (guineafowl). Pelecanus cf. P. onocrotalus is represented by, among other remains, a well-preserved partial skull. Four of the birds are thus referrable to extant taxa that provide some paleoenvironmental clues for Member I of the Kibish Formation. The two species of pelican, the darter, and the heron indicate the presence of local freshwater bodies, a lake or a slow river, supporting resources of fish. The guineafowl is poorly informative ecologically, but probably excludes the notion that the local terrestrial landscape was treeless.