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Sample records for hybridization fish technique

  1. Development of a fluorescent in situ hybridization (FISH) technique for visualizing CGMMV in plant tissues.

    Science.gov (United States)

    Shargil, D; Zemach, H; Belausov, E; Lachman, O; Kamenetsky, R; Dombrovsky, A

    2015-10-01

    Cucumber green mottle mosaic virus (CGMMV), which belongs to the genus Tobamovirus, is a major pathogen of cucurbit crops grown indoors and in open fields. Currently, immunology (e.g., ELISA) and molecular amplification techniques (e.g., RT-PCR) are employed extensively for virus detection in plant tissues and commercial seed lots diagnostics. In this study, a fluorescent in situ hybridization (FISH) technique, using oligonucleotides whose 5'-terminals were labeled with red cyanine 3 (Cy3) or green fluorescein isothiocyanate (FITC), was developed for the visualization of the pathogen in situ. This simple and reliable method allows detection and localization of CGMMV in the vegetative and reproductive tissues of cucumber and melon. When this technique was applied in male flowers, anther tissues were found to be infected; whereas the pollen grains were found to be virus-free. These results have meaningful epidemiological implications for the management of CGMMV, particularly with regard to virus transfer via seed and the role of insects as CGMMV vectors.

  2. Marine Fish Hybridization

    KAUST Repository

    He, Song

    2017-04-01

    Natural hybridization is reproduction (without artificial influence) between two or more species/populations which are distinguishable from each other by heritable characters. Natural hybridizations among marine fishes were highly underappreciated due to limited research effort; it seems that this phenomenon occurs more often than is commonly recognized. As hybridization plays an important role in biodiversity processes in the marine environment, detecting hybridization events and investigating hybridization is important to understand and protect biodiversity. The first chapter sets the framework for this disseration study. The Cohesion Species Concept was selected as the working definition of a species for this study as it can handle marine fish hybridization events. The concept does not require restrictive species boundaries. A general history and background of natural hybridization in marine fishes is reviewed during in chapter as well. Four marine fish hybridization cases were examed and documented in Chapters 2 to 5. In each case study, at least one diagnostic nuclear marker, screened from among ~14 candidate markers, was found to discriminate the putative hybridizing parent species. To further investigate genetic evidence to support the hybrid status for each hybrid offspring in each case, haploweb analysis on diagnostic markers (nuclear and/or mitochondrial) and the DAPC/PCA analysis on microsatellite data were used. By combining the genetic evidences, morphological traits, and ecological observations together, the potential reasons that triggered each hybridization events and the potential genetic/ecology effects could be discussed. In the last chapter, sequences from 82 pairs of hybridizing parents species (for which COI barcoding sequences were available either on GenBank or in our lab) were collected. By comparing the COI fragment p-distance between each hybridizing parent species, some general questions about marine fish hybridization were discussed: Is

  3. Distant hybridization leads to different ploidy fishes

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    Distant hybridization makes it possible to transfer the genome of one species to another, which results in changes in phenotypes and genotypes of the progenies. This study shows that distant hybridization or the combination of this method with gynogenesis or androgenesis lead to different ploidy fishes with genetic variation, including fertile tetraploid hybrids, sterile triploid hybrids, fertile diploid hybrids, fertile diploid gynogenetic fish, and their derived progenies. The formations of the different ploidy fishes depend on the genetic relationship between the parents. In this study, several types of distant hybridization, including red crucian carp (Carassius auratus red var.) (2n=100, abbreviated as RCC) (♀)×common carp (Cyprinus carpio L.) (2n=100, abbreviated as CC) (♂), and RCC (2n=100) (♀)×blunt snout bream (Megalobrama amblycephala) (2n=48, abbreviated as BSB) (♂) are described. In the distant hybridization of RCC (♀)×CC (♂), bisexual fertile F3–F18 allotetraploid hybrids (4n=200, abbreviated as 4nAT) were formed. The diploid hybrid eggs and diploid sperm generated by the females and males of 4nAT developed into diploid gynogenetic hybrids and diploid androgenetic hybrids, respectively, by gynogenesis and androgenesis, without treatment for doubling the chromosome. Improved tetraploid hybrids and improved diploid fishes with genetic variation were derived from the gynogenetic hybrid line. The improved diploid fishes included the high-body RCC and high-body goldfish. The formation of the tetraploid hybrids was related to the occurrence of unreduced gametes generated from the diploid hybrids, which involved in premeiotic endoreduplication, endomitosis, or fusion of germ cells. The sterile triploid hybrids (3n=150) were produced on a large scale by crossing the males of tetraploid hybrids with females of diploid fish (2n=100). In another distant hybridization of RCC (♀)×BSB (♂), different ploidy fishes were obtained, including

  4. Distant hybridization leads to different ploidy fishes.

    Science.gov (United States)

    Liu, ShaoJun

    2010-04-01

    Distant hybridization makes it possible to transfer the genome of one species to another, which results in changes in phenotypes and genotypes of the progenies. This study shows that distant hybridization or the combination of this method with gynogenesis or androgenesis lead to different ploidy fishes with genetic variation, including fertile tetraploid hybrids, sterile triploid hybrids, fertile diploid hybrids, fertile diploid gynogenetic fish, and their derived progenies. The formations of the different ploidy fishes depend on the genetic relationship between the parents. In this study, several types of distant hybridization, including red crucian carp (Carassius auratus red var.) (2n=100, abbreviated as RCC) (female) x common carp (Cyprinus carpio L.) (2n=100, abbreviated as CC) (male), and RCC (2n=100) (female) x blunt snout bream (Megalobrama amblycephala) (2n=48, abbreviated as BSB) (male) are described. In the distant hybridization of RCC (female) x CC (male), bisexual fertile F(3)-F(18) allotetraploid hybrids (4n=200, abbreviated as 4nAT) were formed. The diploid hybrid eggs and diploid sperm generated by the females and males of 4nAT developed into diploid gynogenetic hybrids and diploid androgenetic hybrids, respectively, by gynogenesis and androgenesis, without treatment for doubling the chromosome. Improved tetraploid hybrids and improved diploid fishes with genetic variation were derived from the gynogenetic hybrid line. The improved diploid fishes included the high-body RCC and high-body goldfish. The formation of the tetraploid hybrids was related to the occurrence of unreduced gametes generated from the diploid hybrids, which involved in premeiotic endoreduplication, endomitosis, or fusion of germ cells. The sterile triploid hybrids (3n=150) were produced on a large scale by crossing the males of tetraploid hybrids with females of diploid fish (2n=100). In another distant hybridization of RCC (female) x BSB (male), different ploidy fishes were

  5. Hybrid seine for full fish community collections

    Science.gov (United States)

    McKenna, James E.; Waldt, Emily M.; Abbett, Ross; David, Anthony; Snyder, James

    2013-01-01

    Seines are simple and effective fish collection gears, but the net mesh size influences how well the catch represents the fish communities. We designed and tested a hybrid seine with a dual-mesh bag (1/4″ and 1/8″) and compared the fish assemblage collected by each mesh. The fine-mesh net retained three times as many fish and collected more species (as many as eight), including representatives of several rare species, than did the coarser mesh. The dual-mesh bag permitted us to compare both sizes and species retained by each layer and to develop species-specific abundance correction factors, which allowed comparison of catches with the coarse-mesh seine used for earlier collections. The results indicate that a hybrid seine with coarse-mesh wings and a fine-mesh bag would enhance future studies of fish communities, especially when small-bodied fishes or early life stages are the research focus.

  6. Metaphase FISH on a Chip: Miniaturized Microfluidic Device for Fluorescence in situ Hybridization

    DEFF Research Database (Denmark)

    Vedarethinam, Indumathi; Shah, Pranjul Jaykumar; Dimaki, Maria;

    2010-01-01

    Fluorescence in situ Hybridization (FISH) is a major cytogenetic technique for clinical genetic diagnosis of both inherited and acquired chromosomal abnormalities. Although FISH techniques have evolved and are often used together with other cytogenetic methods like CGH, PRINS and PNA-FISH, the pr......Fluorescence in situ Hybridization (FISH) is a major cytogenetic technique for clinical genetic diagnosis of both inherited and acquired chromosomal abnormalities. Although FISH techniques have evolved and are often used together with other cytogenetic methods like CGH, PRINS and PNA...

  7. Molecular cytogenetics of pituitary adenomas, assessed by FISH technique.

    Science.gov (United States)

    Kontogeorgos, George

    2004-01-01

    Fluorescent in situ hybridization (FISH) represents a moden molecular pathology technique, alternative to conventional cytogenetics (karyotyping). In addition to metaphase spreads, it can be applied directly to interphase nuclei. The latter makes the FISH technique powerful for pathologists for it integrates molecular genetics and classic cytogenetics and brings them together to a single framework for morphologic evaluation. Interphase FISH can be applied to imprints from fresh tissue or to paraffin sections after proteinase K digestion. Centromeric, telomeric and locus DNA-sequence specific probes can be used to identify aneuploidy or gene mutations. Several protocols combine molecular cytogenetics with classic karyotyping. Other sophisticated, FISH-based protocols have been introduced. Among them, comparative genomic hybridization is very important for it can detect non-balanced chromosomal aberrations of uncultured tumor cells and provide overall genomic information in a single experiment. This review presents the principles and applications of FISH technique for the investigation of the cytogenetic background of pituitary adenomas.

  8. Application of fluorescence in situ hybridization (FISH) to the ...

    African Journals Online (AJOL)

    Application of fluorescence in situ hybridization (FISH) to the analysis of ... In this study, fluorescent in situ hybridization (FISH) as a culture-independent molecular ... a high percentage and took place in an oily biological system under aerobic ...

  9. The application of fluorescence in situ hybridization (FISH technique for studying the microbial communities in intestinal tissues of white shrimp (Penaeus vannamei

    Directory of Open Access Journals (Sweden)

    Supamattaya, K.

    2005-02-01

    Full Text Available Fluorescence in situ hybridization technique is very useful for the evaluation of microbial communities in various environments. It is possible to apply this technique to study the intestinal microflora in white shrimp (Penaeus vannamei. Different fixatives and storage temperature were tested in this technique. It was found that fixation with 10% buffered formalin for 12 hours and changed to 70% ethanol shown positive results when compared to the fixation with Davidson's fixative or RF fixative. The best signaling was obtainedfrom the samples which were stored in -20ºC. By using the DNA probe targeted to the Eubacteria domain (EUB338 probe, 5′-GCT GCC TCC CGT AGG AGT-3′ labeled with fluorescein as a hybridizing probe, it was found that most intestinal microflora were aggregated with the intestinal contents, or dispersed in the lumen. There was not evidence of the attachment of the microflora with the intestinal epithelium in this study.

  10. Sugar Cane Genome Numbers Assumption by Ribosomal DNA FISH Techniques

    NARCIS (Netherlands)

    Thumjamras, S.; Jong, de H.; Iamtham, S.; Prammanee, S.

    2013-01-01

    Conventional cytological method is limited for polyploidy plant genome study, especially sugar cane chromosomes that show unstable numbers of each cultivar. Molecular cytogenetic as fluorescent in situ hybridization (FISH) techniques were used in this study. A basic chromosome number of sugar cane

  11. Histopathology of fish: I. Techniques and principles

    Science.gov (United States)

    Wood, E.M.; Yasutake, W.T.

    1955-01-01

    The techniques of histopathology have been used for many years in the study of human and animal diseases. Until very recent times, however, histology has been applied to fish studies only very infrequently. This brief discussion is intended to acquaint the reader with the techniques and principles involved and to explain how histological studies may help to overcome fish diseases and nutritional problems.

  12. [Progress in transgenic fish techniques and application].

    Science.gov (United States)

    Ye, Xing; Tian, Yuan-Yuan; Gao, Feng-Ying

    2011-05-01

    Transgenic technique provides a new way for fish breeding. Stable lines of growth hormone gene transfer carps, salmon and tilapia, as well as fluorescence protein gene transfer zebra fish and white cloud mountain minnow have been produced. The fast growth characteristic of GH gene transgenic fish will be of great importance to promote aquaculture production and economic efficiency. This paper summarized the progress in transgenic fish research and ecological assessments. Microinjection is still the most common used method, but often resulted in multi-site and multi-copies integration. Co-injection of transposon or meganuclease will greatly improve the efficiency of gene transfer and integration. "All fish" gene or "auto gene" should be considered to produce transgenic fish in order to eliminate misgiving on food safety and to benefit expression of the transferred gene. Environmental risk is the biggest obstacle for transgenic fish to be commercially applied. Data indicates that transgenic fish have inferior fitness compared with the traditional domestic fish. However, be-cause of the genotype-by-environment effects, it is difficult to extrapolate simple phenotypes to the complex ecological interactions that occur in nature based on the ecological consequences of the transgenic fish determined in the laboratory. It is critical to establish highly naturalized environments for acquiring reliable data that can be used to evaluate the environ-mental risk. Efficacious physical and biological containment strategies remain to be crucial approaches to ensure the safe application of transgenic fish technology.

  13. Marine genetic swamping: hybrids replace an obligately estuarine fish.

    Science.gov (United States)

    Roberts, David G; Gray, Charles A; West, Ronald J; Ayre, David J

    2010-02-01

    Populations of obligately estuarine taxa are potentially small and isolated and may lack genetic variation and display regional differentiation as a result of drift and inbreeding. Hybridization with a wide-ranging marine congener should introduce genetic variation and reduce the effects of inbreeding depression and genetic drift. However, high levels of hybridization can cause demographic and genetic swamping. In southeastern Australia hybridization occurs between obligately estuarine Black bream (Acanthopagrus butcheri) and migratory marine Yellowfin bream (Acanthopagrus australis). Here, we surveyed genetic variation at eight microsatellite loci and the mitochondrial control region of juvenile fish from five coastal lagoons (including temporal replication in two lagoons) (total n = 970) to determine the frequency and persistence of hybridization, and its likely consequence for the estuarine restricted A. butcheri. Of 688 juvenile fish genotyped 95% were either A. australis (347) or hybrids (309); only 5% (32) were A. butcheri. Most hybrids were later generation hybrids or A. butcheri backcrosses, which are likely multi-generational residents within lagoons. Far greater proportions of hybrid juveniles were found within two lagoons that are generally closed to the ocean (>90% hybrid fish within generally closed lagoons vs. 12-27% in permanently or intermittently open lagoons). In both lagoons, this was consistent across multiple cohorts of fish [79-97% hybrid fish (n = 282)]. Hybridization and introgression represent a major threat to the persistence of A. butcheri and have yet to be investigated for large numbers of estuarine taxa.

  14. Fish Spoilage Mechanisms and Preservation Techniques: Review

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    Abdel E. Ghaly

    2010-01-01

    Full Text Available Problem statement: Spoilage of food products is due to chemical, enzymatic or microbial activities One-fourth of the worlds food supply and 30% of landed fish are lost through microbial activity alone. With the ever growing world population and the need to store and transport the food from one place to another where it is needed, food preservation becomes necessary in order to increase its shelf life and maintain its nutritional value, texture and flavor. The freshness and quality of fish have always gained the attention by Food Regulatory Agencies and Food Processing Industry. Proper handling, pretreatment and preservation techniques can improve the quality fish and fish products and increase their shelf life. Methodology: Historically salting, drying, smoking, fermentation and canning were the methods to prevent fish spoilage and extend its shelf life. In response to consumer demand for texture, appearance and taste, new methods were developed including: Cooling, freezing and chemical preservation. A comprehensive review of the literature on the subject of fish spoilage and modern preservation techniques was carried out. Conclusion: Fish spoilage results from three basic mechanisms: Enzymatic autolysis, oxidation, microbial growth. Low temperature storage and chemical techniques for controlling water activity, enzymatic, oxidative and microbial spoilage are the most common in the industry today. A process involving the addition of an EDTA (1 mM-TBHQ (0.02% combination and ascorbic acid and storage at refrigerated temperatures (5°C in darkness can be the most positive for controlling the spoilage of fish and fish product. The suggested process would address antimicrobial activity as well as destructive oxidation of the desired lipids and fats. However, more efforts are required to understand the role of proximate composition of fish, post harvest history, environmental conditions, initial microbial load, type and nature of bacteria and their

  15. Fluorescence in situ hybridization(FISH) technique combined with karyotyping analysis in prenatal diagnosis%FISH技术在产前诊断中的应用价值研究

    Institute of Scientific and Technical Information of China (English)

    沈国松; 张甦; 何平亚; 方嵘

    2011-01-01

    目的 使用荧光原位杂交(FISH)技术对常见胎儿染色体数目异常进行快速诊断,结合羊水细胞培养染色体核型分析技术形成产前诊断体系,并对其临床应用价值进行评价.方法 应用诊断最常见染色体病的5种染色体(13、18、21、X和Y)特异性FISH探针对480例未经培养羊水细胞进行产前诊断,并和同时进行的羊水培养染色体核型分析相比较.结果 480例未经培养羊水细胞FISH实验全部获得检测结果,并发现21-三体综合征2例,18-三体综合征1例,克氏综合征1例,特纳综合征 1例,与羊水培养染色体分析结果一致,但报告时间(2至3d)与羊水染色体分析报告时间(2~3周)相比大为缩短.受固有技术限制有6例结构异常未能检出,但应用FISH技术对其中1例与性染色体有关的结构异常进行辅助诊断,获得了成功.结论 FISH技术在常见染色体数目异常产前诊断中应用行之有效,与羊水染色体核型分析技术相结合,将使产前诊断更加高效和安全.%To evaluate the clinical application of the combination of fluorescence in situ hybridization (FISH)technique and karyotyping analysis in prenatal diagnosis.MethodsFISH technology with the centromeric probes of chromo-some 13, 18, 21 ,X and Y was applied in 480 amniotic fluid specimens, the results were compared with those of the G banding karyotypes from standard cytogenetic analysis in cultured amniotic fluid cells.ResultsTotal 480 uncultured amniotic fluid speci-mens were successfully tested by FISH and two Down' s syndrome, one Edwards syndrome, one Klinefelter syndrome and one Turner syndrome were detected. The results obtained by FISH were consistent with those from karyotyping analysis. FISH (2-3 days) was faster than karyotyping(2-3 weeks) to get results. Karyotypes analysis detected 6 cases of chromosome structural abnormalities which were not detected by FISH, however, one of them with a sex chromosome-related structural

  16. Multiplex-FISH (M-FISH): technique, developments and applications.

    Science.gov (United States)

    Kearney, L

    2006-01-01

    Multiplex FISH (M-FISH) represents one of the most significant developments in molecular cytogenetics of the past decade. Originally designed to generate 24 colour karyotyping, the technique has spawned many variations and an equally diverse range of applications. In tumour and leukaemia cytogenetics, the two groups that have been targeted represent both ends of the cytogenetic spectrum: those with an apparently normal karyotype (suspected of harbouring small rearrangements not detectable by conventional cytogenetics) and those with a complex aberrant karyotype (which are difficult to karyotype accurately due to the sheer number of aberrations). In research, mouse M-FISH provides a powerful tool to characterize mouse models of a disease. In addition, the ability to accurately karyotype single metaphases without selection makes M-FISH the perfect tool in chromosome breakage studies and for characterizing clonal evolution of tumours. Finally, M-FISH has emerged as the perfect partner for the developing genomic microarray (array CGH) technologies, providing a powerful approach to gene discovery.

  17. DNA breakage detection-fluorescence in situ hybridization (DBD-FISH in buccal cells

    Directory of Open Access Journals (Sweden)

    E. I. Cortés-Gutiérrez

    2012-12-01

    Full Text Available DNA breakage detection-fluorescence in situ hybridization (DBD-FISH is a recently developed technique that allows cell-by-cell detection and quantification of DNA breakage in the whole genome or within specific DNA sequences. The present investigation was conducted to adapt the methodology of DBD-FISH to the visualization and evaluation of DNA damage in buccal epithelial cells. DBD-FISH revealed that DNA damage increased significantly according to H2O2 concentration (r2=0.91. In conclusion, the DBD-FISH technique is easy to apply in buccal cells and provides prompt results that are easy to interpret. Future studies are needed to investigate the potential applicability of a buccal cell DBD-FISH model to human biomonitoring and nutritional work.

  18. DNA breakage detection-fluorescence in situ hybridization (DBD-FISH) in buccal cells.

    Science.gov (United States)

    Cortés-Gutiérrez, E I; Dávila-Rodríguez, M I; Fernández, J L; López-Fernández, C; Gosálvez, J

    2012-12-28

    DNA breakage detection-fluorescence in situ hybridization (DBD-FISH) is a recently developed technique that allows cell-by-cell detection and quantification of DNA breakage in the whole genome or within specific DNA sequences. The present investigation was conducted to adapt the methodology of DBD-FISH to the visualization and evaluation of DNA damage in buccal epithelial cells. DBD-FISH revealed that DNA damage increased significantly according to H2O2 concentration (r2=0.91). In conclusion, the DBD-FISH technique is easy to apply in buccal cells and provides prompt results that are easy to interpret. Future studies are needed to investigate the potential applicability of a buccal cell DBD-FISH model to human biomonitoring and nutritional work.

  19. Metaphase FISH on a Chip: Miniaturized Microfluidic Device for Fluorescence in situ Hybridization

    Directory of Open Access Journals (Sweden)

    Niels Tommerup

    2010-11-01

    Full Text Available Fluorescence in situ Hybridization (FISH is a major cytogenetic technique for clinical genetic diagnosis of both inherited and acquired chromosomal abnormalities. Although FISH techniques have evolved and are often used together with other cytogenetic methods like CGH, PRINS and PNA-FISH, the process continues to be a manual, labour intensive, expensive and time consuming technique, often taking over 3–5 days, even in dedicated labs. We have developed a novel microFISH device to perform metaphase FISH on a chip which overcomes many shortcomings of the current laboratory protocols. This work also introduces a novel splashing device for preparing metaphase spreads on a microscope glass slide, followed by a rapid adhesive tape-based bonding protocol leading to rapid fabrication of the microFISH device. The microFISH device allows for an optimized metaphase FISH protocol on a chip with over a 20-fold reduction in the reagent volume. This is the first demonstration of metaphase FISH on a microfluidic device and offers a possibility of automation and significant cost reduction of many routine diagnostic tests of genetic anomalies.

  20. Ancient hybridization and genomic stabilization in a swordtail fish.

    Science.gov (United States)

    Schumer, Molly; Cui, Rongfeng; Powell, Daniel L; Rosenthal, Gil G; Andolfatto, Peter

    2016-06-01

    A rapidly increasing body of work is revealing that the genomes of distinct species often exhibit hybrid ancestry, presumably due to postspeciation hybridization between closely related species. Despite the growing number of documented cases, we still know relatively little about how genomes evolve and stabilize following hybridization, and to what extent hybridization is functionally relevant. Here, we examine the case of Xiphophorus nezahualcoyotl, a teleost fish whose genome exhibits significant hybrid ancestry. We show that hybridization was relatively ancient and is unlikely to be ongoing. Strikingly, the genome of X. nezahualcoyotl has largely stabilized following hybridization, distinguishing it from examples such as human-Neanderthal hybridization. Hybridization-derived regions are remarkably distinct from other regions of the genome, tending to be enriched in genomic regions with reduced constraint. These results suggest that selection has played a role in removing hybrid ancestry from certain functionally important regions. Combined with findings in other systems, our results raise many questions about the process of genomic stabilization and the role of selection in shaping patterns of hybrid ancestry in the genome. © 2016 John Wiley & Sons Ltd.

  1. Parasitic fauna in hybrid tambacu from fish farms

    Directory of Open Access Journals (Sweden)

    Ronilson Macedo Silva

    2013-08-01

    Full Text Available The objective of this work was to evaluate the parasitic fauna of hybrid tambacu (Colossoma macropomum x Piaractus mesopotamicus from fish farms and the host-parasite relationship. A hundred and fourteen fish were collected from four fish farms in Macapá, in the state of Amapá, Brazil, 80.7% of which were infected by: Ichthyophthirius multifiliis (Ciliophora; Piscinoodinium pillulare (Dinoflagellida; Anacanthorus spatulatus, Notozothecium janauachensis, and Mymarothecium viatorum (Monogenoidea; Neoechinorhynchus buttnerae (Acanthocephala; Cucullanus colossomi (Nematoda; Perulernaea gamitanae (Lernaeidae; and Proteocephalidae larvae (Cestoda. A total of 8,136,252 parasites were collected from the examined fish. This is the first record of N. buttnerae, C. colossomi, N. janauachensis, M. viatorum, and Proteocephalidae for hybrid tambacu in Brazil. Ichthyophthirius multifiliis was the most prevalent parasite, whereas endohelminths were the less. A positive correlation was observed between number of I. multifiliis and total length and weight of fish, as well as between number of P. gamitanae and total length. The infection by I. multifiliis had association with the parasitism by Monogenoidea. Low water quality contributes to high parasitism of hybrid tambacu by ectoparasites, which, however, does not influence the relative condition factor of fish.

  2. Interphase cytogenetics in pathology: principles, methods, and applications of fluorescence in situ hybridization (FISH).

    Science.gov (United States)

    Werner, M; Wilkens, L; Aubele, M; Nolte, M; Zitzelsberger, H; Komminoth, P

    1997-01-01

    Characteristic chromosome aberrations have been identified in various tumors. Fluorescence in situ hybridization (FISH) using specific probes that are generated by vector cloning or in vitro amplification and labeled with fluorescent dyes allow for the detection of these genetic changes in interphase cells. This technique, that is also referred to as "interphase cytogenetics", can be performed in cytological preparations as well as in sections of routinely formaldehyde-fixed and paraffin-embedded tissue. In cancer research and diagnostics, interphase cytogenetics by FISH is used to detect numerical chromosome changes and structural aberrations, e.g., translocations, deletions, or amplifications. In this technical overview, we explain the principles of the FISH method and provide protocols for FISH in cytological preparations and paraffin sections. Moreover, possible applications of FISH are discussed.

  3. The chromosomal constitution of fish hybrid lineage revealed by 5S rDNA FISH.

    Science.gov (United States)

    Zhang, Chun; Ye, Lihai; Chen, Yiyi; Xiao, Jun; Wu, Yanhong; Tao, Min; Xiao, Yamei; Liu, Shaojun

    2015-12-03

    The establishment of the bisexual fertile fish hybrid lineage including the allodiploid and allotetraploid hybrids, from interspecific hybridization of red crucian carp (Carassius auratus red var. 2n = 100, 2n = AA) (♀) × common carp (Cyprinus carpio L. 2n = 100, 2n = BB) (♂), provided a good platform to investigate genetic relationship between the parents and their hybrid progenies. The chromosomal inheritance of diploid and allotetraploid hybrid progenies in successive generations, was studied by applying 5S rDNA fluorescence in situ hybridization. Signals of 5S rDNA distinguished the chromosomal constitution of common carp (B-genome) from red crucian carp (A-genome), in which two strong signals were observed on the first submetacentric chromosome, while no major signal was found in common carp. After fish hybridization, one strong signal of 5S rDNA was detected in the same locus on the chromosome of diploid hybrids. As expected, two strong signals were observed in 4nF3 tetraploid hybrids offspring and it is worth mentioning that two strong signals were detected in a separating bivalent of a primary spermatocyte in 4nF3. Furthermore, the mitosis of heterozygous chromosomes was shown normal and stable with blastular tissue histological studies. We revealed that 5S rDNA signal can be applied to discern A-genome from B-genome, and that 5S rDNA bearing chromosomes can be stably passed down in successive generations. Our work provided a significant method in fish breeding and this is important for studies in fish evolutionary biology.

  4. TA3 - Dosimetry and instrumentation supply of the M-Fish technique to the Fish-3 painting technique for analysing translocations: A radiotherapy-treated patient study

    Energy Technology Data Exchange (ETDEWEB)

    Pouzoulet, F.; Roch-Lefevre, S.; Giraudet, A.L.; Vaurijoux, A.; Voisin, P.A.; Buard, V.; Delbos, M.; Voisin, Ph.; Roy, L. [Institut de Radioprotection et de Surete Nucleaire, Lab. de Dosimetrie Biologique, 92 - Fontenay aux Roses (France); Bourhis, J. [Laboratoire UPRES EA 27-10, Radiosensibilite des Tumeurs et Tissus sains, PR1, 94 - Villejuif (France)

    2006-07-01

    Purpose: Currently, the chromosome translocation study is the best method to estimate the dose of an old radiation exposure. Fluorescent In Situ Hybridization (F.I.S.H.) technique allows an easy detection of this kind of aberrations. However, as only a few number of chromosomes is usually painted, some bias could skew the result. To evaluate the advantage of using full genome staining (M-F.I.S.H. technique) compared with three chromosomes labelling (F.I.S.H.-3 painting), we compared translocation yields in radiotherapy treated patients. Methods: Chromosome aberration analyses were performed on peripheral blood lymphocyte cultures of two patients treated for a throat cancer by radiotherapy. Blood samples were obtained, before, along the treatment and six or four months later. For each sample, a dicentrics analysis was performed together with translocation analysis either with F.I.S.H.-3 painting or M-F.I.S.H.. Results: By confronting results from the F.I.S.H.-3 painting technique and the M-F.I.S.H. technique, significant differences were revealed. The translocations yield seemed to be stable with the F.I.S.H.-3 painting technique whereas it is not the case with the M-F.I.S.H. technique. This difference in results was explained by the bias induced by F.I.S.H.-3 Painting technique in the visualisation of complex aberrations. Furthermore, we found the presence of a clone bearing a translocation involving a painted chromosome. Conclusions: According to the potential bias of F.I.S.H.-3 painting on translocations study, the M-F.I.S.H. technique should provide more precise and reproducible results. Because of its more difficult implement, it seems hardly applicable to retrospective dosimetry instead of F.I.S.H.-3 painting technique. (authors)

  5. itFISH: Enhanced Staining by Iterative Fluorescent In Situ Hybridization.

    Science.gov (United States)

    Row, Richard H; Martin, Benjamin L

    2017-03-20

    Fluorescent in situ hybridization (FISH) is an important tool for zebrafish research, particularly when observing the expression of two different genes in the same embryo. Peroxidase-catalyzed deposition of tyramide-conjugated dyes is a widely used and cost-effective approach to performing FISH. A major limitation of the technique is that it does not work well for weakly expressed genes. Here we present a method adapted from planarian research for use in zebrafish that provides a dramatic enhancement of weak staining. By iterating the antibody staining and development steps, a strong signal can be obtained from probes that were previously too weak to detect.

  6. Study of the Artificial Fish Swarm Algorithm for Hybrid Clustering

    Directory of Open Access Journals (Sweden)

    Hongwei Zhao

    2015-06-01

    Full Text Available The basic Artificial Fish Swarm (AFS Algorithm is a new type of an heuristic swarm intelligence algorithm, but it is difficult to optimize to get high precision due to the randomness of the artificial fish behavior, which belongs to the intelligence algorithm. This paper presents an extended AFS algorithm, namely the Cooperative Artificial Fish Swarm (CAFS, which significantly improves the original AFS in solving complex optimization problems. K-medoids clustering algorithm is being used to classify data, but the approach is sensitive to the initial selection of the centers with low quality of the divided cluster. A novel hybrid clustering method based on the CAFS and K-medoids could be used for solving clustering problems. In this work, first, CAFS algorithm is used for optimizing six widely-used benchmark functions, coming up with comparative results produced by AFS and CAFS, then Particle Swarm Optimization (PSO is studied. Second, the hybrid algorithm with K-medoids and CAFS algorithms is used for data clustering on several benchmark data sets. The performance of the hybrid algorithm based on K-medoids and CAFS is compared with AFS and CAFS algorithms on a clustering problem. The simulation results show that the proposed CAFS outperforms the other two algorithms in terms of accuracy and robustness.

  7. Ancient hybridization fuels rapid cichlid fish adaptive radiations

    Science.gov (United States)

    Meier, Joana I.; Marques, David A.; Mwaiko, Salome; Wagner, Catherine E.; Excoffier, Laurent; Seehausen, Ole

    2017-01-01

    Understanding why some evolutionary lineages generate exceptionally high species diversity is an important goal in evolutionary biology. Haplochromine cichlid fishes of Africa's Lake Victoria region encompass >700 diverse species that all evolved in the last 150,000 years. How this ‘Lake Victoria Region Superflock' could evolve on such rapid timescales is an enduring question. Here, we demonstrate that hybridization between two divergent lineages facilitated this process by providing genetic variation that subsequently became recombined and sorted into many new species. Notably, the hybridization event generated exceptional allelic variation at an opsin gene known to be involved in adaptation and speciation. More generally, differentiation between new species is accentuated around variants that were fixed differences between the parental lineages, and that now appear in many new combinations in the radiation species. We conclude that hybridization between divergent lineages, when coincident with ecological opportunity, may facilitate rapid and extensive adaptive radiation. PMID:28186104

  8. Technique for Measuring Hybrid Electronic Component Reliability

    Energy Technology Data Exchange (ETDEWEB)

    Green, C.C.; Hernandez, C.L.; Hosking, F.M.; Robinson, D.; Rutherford, B.; Uribe, F.

    1999-01-01

    Materials compatibility studies of aged, engineered materials and hardware are critical to understanding and predicting component reliability, particularly for systems with extended stockpile life requirements. Nondestructive testing capabilities for component reliability would significantly enhance lifetime predictions. For example, if the detection of crack propagation through a solder joint can be demonstrated, this technique could be used to develop baseline information to statistically determine solder joint lifelengths. This report will investigate high frequency signal response techniques for nondestructively evaluating the electrical behavior of thick film hybrid transmission lines.

  9. Detection of chromosome aberrations in interphase nuclei using fluorescence in situ hybridization technique.

    OpenAIRE

    1993-01-01

    We report here several experiences of interphase cytogenetics, using fluorescence in situ hybridization (FISH) technique, for the detection of chromosome aberrations. FISH, using alpha satellite specific probes of 18, X, Y chromosomes, was done in interphase nuclei from peripheral blood of patients with Edwards' syndrome, Klinefelter's syndrome and Turner's syndrome with healthy male and female controls, respectively. The distributions of fluorescent signals in 100 interphase nuclei were well...

  10. Optimum prey capture techniques in fish

    NARCIS (Netherlands)

    Leeuwen, van J.L.

    1983-01-01

    In this thesis hydrodynamic principles are used to quantify relations between form and function in the prey capture mechanism of actinopterygian fish. This work is closely related to the papers on the hydrodynamics of fish feeding by Muller et al. (1982) and Muller & Osse (in press).

  11. Chromogenic in situ hybridization: a multicenter study comparing silver in situ hybridization with FISH.

    Science.gov (United States)

    Bartlett, J M S; Campbell, Fiona M; Ibrahim, Merdol; Wencyk, Peter; Ellis, Ian; Kay, Elaine; Connolly, Yvonne; O'Grady, Anthony; Di Palma, Silvana; Starczynski, Jane; Morgan, John M; Jasani, Bharat; Miller, Keith

    2009-10-01

    Our purposes were to perform a robust assessment of a new HER2 chromogenic in situ hybridization test and report on concordance of silver in situ hybridization (SISH) data with fluorescence in situ hybridization (FISH) data and on intraobserver and interlaboratory scoring consistency. HER2 results were scored from 45 breast cancers in 7 laboratories using the Ventana (Tucson, AZ) INFORM HER-2 SISH assay and in 1 central laboratory using a standard FISH assay. Overall, 94.8% of cases were successfully analyzed by SISH across the 6 participating laboratories that reported data. Concordance for diagnosis of HER2 amplification by SISH compared with FISH was high (96.0% overall). Intraobserver variability (8.0%) and intersite variability (12.66%) of absolute HER2/chromosome 17 ratios appear to be tightly controlled across all 6 participating laboratories. The Ventana INFORM HER-2 SISH assay is robust and reproducible, shows good concordance with a standard FISH assay, and complies with requirements in national guidelines for performance of diagnostic tests.

  12. Hybrid ultrasound imaging techniques (fusion imaging).

    Science.gov (United States)

    Sandulescu, Daniela Larisa; Dumitrescu, Daniela; Rogoveanu, Ion; Saftoiu, Adrian

    2011-01-07

    Visualization of tumor angiogenesis can facilitate non-invasive evaluation of tumor vascular characteristics to supplement the conventional diagnostic imaging goals of depicting tumor location, size, and morphology. Hybrid imaging techniques combine anatomic [ultrasound, computed tomography (CT), and/or magnetic resonance imaging (MRI)] and molecular (single photon emission CT and positron emission tomography) imaging modalities. One example is real-time virtual sonography, which combines ultrasound (grayscale, colour Doppler, or dynamic contrast harmonic imaging) with contrast-enhanced CT/MRI. The benefits of fusion imaging include an increased diagnostic confidence, direct comparison of the lesions using different imaging modalities, more precise monitoring of interventional procedures, and reduced radiation exposure.

  13. Fish Spoilage Mechanisms and Preservation Techniques: Review

    OpenAIRE

    Abdel E. Ghaly; Dave, D; Budge, S; M. S. Brooks

    2010-01-01

    Problem statement: Spoilage of food products is due to chemical, enzymatic or microbial activities One-fourth of the worlds food supply and 30% of landed fish are lost through microbial activity alone. With the ever growing world population and the need to store and transport the food from one place to another where it is needed, food preservation becomes necessary in order to increase its shelf life and maintain its nutritional value, texture and flavor. The freshness and quality of fish hav...

  14. Fluorescent in situ hybridization (FISH on the veterinary diagnostic field

    Directory of Open Access Journals (Sweden)

    Ján Dianovský

    2016-09-01

    Full Text Available Proceeding deals with of genomic changes detectable by FISH . The DSD syndrom in Yorkshire terrier 78,XY t (Y-;6p+ was observed by the use of X and Y FISH WCP probes. Following results indicated numerous genomic changes in cancers. Using comparative genomic hybridization numerous chromosomal rearrangements were detected, which indicated the heterogeneity in tumour growth. In Bernese Mountain Dog bitch,8 loses on chromosomes and gains on 18 different of chromosomes were detected. The last study was focused on chromosomal position and nucleotide sequencing of the LCA5L exons. Those were analysed in cattle of BTA1q44, sheep OAR1q43 and of CHI1q44 in the goats.

  15. HER2 in situ hybridization in gastric and gastroesophageal adenocarcinoma: comparison of automated dual ISH to FISH.

    Science.gov (United States)

    Grin, Andrea; Brezden-Masley, Christine; Bauer, Sharon; Streutker, Catherine J

    2013-12-01

    Patients with gastric and gastroesophageal junction (GEJ) adenocarcinomas that are HER2 positive by immunohistochemistry (IHC) or in situ hybridization show a significant survival benefit with trastuzumab therapy. In situ hybridization is traditionally done by fluorescence in situ hybridization (FISH), despite some limitations. An alternative is the dual in situ hybridization (Dual ISH) technique that is fully automated and uses differentially labeled CEP17 and HER2 probes that can be read by light microscopy on 1 slide. The aim of this study was to assess the utility of Dual ISH in gastric/GEJ cancer and to compare the results with those obtained by IHC and FISH. Cases of gastric/GEJ adenocarcinoma were analyzed by IHC, FISH, and Dual ISH and the correlation between methods calculated. Results for 50 patients were available. There was a 98% (49/50) concordance rate between Dual ISH and FISH. One discrepant case was nonamplified by FISH but showed focal amplification by Dual ISH. Discrepancy was attributed to tumor heterogeneity, which was a frequent finding (78% of HER2-positive cases). There was excellent correlation between Dual ISH and FISH for assessment of HER2 amplification. Dual ISH was rapid, easy to interpret, and maintained cell morphology, which was valuable in identifying tumor heterogeneity.

  16. A New Type of Hybrid Fish-like Microrobot

    Institute of Scientific and Technical Information of China (English)

    Wei Zhang; Shu-Xiang Guo; Kinji Asaka

    2006-01-01

    In order to develop a new type of fish-like microrobot with swimming, walking, and floating motions, in our past research, we developed a hybrid microrobot actuated by ionic conducting polymer film (ICPF) actuators. But the microrobot had some problems in walking and floating motions. In this paper, we propose a concept of hybrid microrobot (see Fig. 1). The microrobot is actuated by a pair of caudal fins, a base with legs and an array of artificial swim bladders. We have developed a prototype of the base with legs and one artificial swim bladder, respectively, and carried out experiments for evaluating their characteristics. Experimental results show the base with legs can realize walking speed of 6 mm/s and rotating speed of 7.1 degrees/s respectively, and the prototype of the artificial swim bladder has a maximum floatage of 2.6 mN. The experimental results also indicate that the microrobot has some advantages, such as walking motion with 2 degrees of freedom, the walking ability on rough surface (sand paper), the controllable floatage, etc. This kind of fish-like microrobot is expected for industrial and medical applications.

  17. An ordination technique for fish diet comparisons

    Directory of Open Access Journals (Sweden)

    Tadeusz Penczak

    2000-01-01

    Full Text Available The frequency of nine food types in the guts of 58 fish species from the Paraná River and its floodplain was compared using similarity definition. A synthetic table ordered on the basis of this definition, with fish species on axis y, and food types on axis x, enabled easy reading numerous kinds of information on feeding ecology of fish in the catchment. For example: 1 which fish species were generalists, specialists or opportunists, 2 those classified as herbivorous species consumed small amounts of animal food, 3 that fish consumed any food type that contained transformed solar energy and matter, 4 what are food preferences dependent on fish taxonomic affinity. The possibility of employing synthetic tables for investigating spatial and temporal variation in fish diet has also been indicated.As freqüência de ocorrência de nove tipos de itens alimentares em estômagos de 58 espécies de peixes do rio Paraná e sua planície alagável são comparadas com base nas definições de similaridade. É proposta uma tabela sintética baseada nessas definições, com as espécies de peixes no eixo y e os tipos de alimento no eixo x, permitindo uma visualização mais clara de diversos tipos de informações sobre a ecologia alimentar de peixes na bacia. Entre as informações, destacam-se (i quais espécies são generalistas, especialistas ou oportunistas; (ii que espécies classificadas como herbívoras consomem também itens de origem animal; (iii que os peixes consomem qualquer tipo de alimento que contenha energia; (iv que a preferência alimentar depende das afinidades taxonômicas. A possibilidade de empregar tabelas sintéticas na investigação de variações espaciais e temporais na dieta de peixe é também indicada.

  18. Evaluation of fish handling techniques employed by artisanal fishers ...

    African Journals Online (AJOL)

    Evaluation of fish handling techniques employed by artisanal fishers on quality of ... genera at landing time along the Kenyan coast using sensory and microbiological methods. ... African Journal of Food, Agriculture, Nutrition and Development.

  19. Hybrid ultrasound imaging techniques(fusion imaging)

    Institute of Scientific and Technical Information of China (English)

    Daniela Larisa Sandulescu; Daniela Dumitrescu; Ion Rogoveanu; Adrian Saftoiu

    2011-01-01

    Visualization of tumor angiogenesis can facilitate noninvasive evaluation of tumor vascular characteristics to supplement the conventional diagnostic imaging goals of depicting tumor location,size,and morphology.Hybrid imaging techniques combine anatomic [ultrasound,computed tomography(CT),and/or magnetic resonance imaging(MRI)] and molecular(single photon emission CT and positron emission tomography)imaging modalities.One example is real-time virtual sonography,which combines ultrasound(grayscale,colour Doppler,or dynamic contrast harmonic imaging)with contrast-enhanced CT/MRI.The benefits of fusion imaging include an increased diagnostic confidence,direct comparison of the lesions using different imaging modalities,more precise monitoring of interventional procedures,and reduced radiation exposure.

  20. HYBRID INTERNET TRAFFIC CLASSIFICATION TECHNIQUE1

    Institute of Scientific and Technical Information of China (English)

    Li Jun; Zhang Shunyi; Lu Yanqing; Yan Junrong

    2009-01-01

    Accurate and real-time classification of network traffic is significant to network operation and management such as QoS differentiation, traffic shaping and security surveillance. However, with many newly emerged P2P applications using dynamic port numbers, masquerading techniques, and payload encryption to avoid detection, traditional classification approaches turn to be ineffective. In this paper, we present a layered hybrid system to classify current Internet traffic, motivated by variety of network activities and their requirements of traffic classification. The proposed method could achieve fast and accurate traffic classification with low overheads and robustness to accommodate both known and unknown/encrypted applications. Furthermore, it is feasible to be used in the context of real-time traffic classification. Our experimental results show the distinct advantages of the proposed classification system, compared with the one-step Machine Learning (ML) approach.

  1. Introgressive hybridization as a promoter of genome reshuffling in natural homoploid fish hybrids (Cyprinidae, Leuciscinae).

    Science.gov (United States)

    Pereira, C S A; Aboim, M A; Ráb, P; Collares-Pereira, M J

    2014-03-01

    Understanding the mechanisms underlying diversification and speciation by introgressive hybridization is currently one of the major challenges in evolutionary biology. Here, the analysis of hybridization between two pairs of Iberian Leuciscinae provided new data on independent hybrid zones involving Achondrostoma oligolepis (AOL) and Pseudochondrostoma duriense (PDU), and confirmed the occurrence of hybrids between AOL and Pseudochondrostoma polylepis (PPO). A multilevel survey combining morphological, genetic and cytogenomic markers on a vast population screening successfully sorted the selected fishes as admixed. Results were similar in both AOL × PDU and AOL × PPO systems. Overall, hybrid morphotypes, cytogenomic data and genetic profiling indicated preferential backcrossing and suggested AOL as a major genomic contributor. Moreover, results implied AOL as more permissive to introgression than PDU or PPO. Although PDU- and PPO-like individuals appeared more resilient to genome modifications, AOL appeared to be more involved and affected by the ongoing hybridization events, as chromosomal translocations were only found in AOL-like individuals. All hybrids analysed evidenced extensive ribosomal DNA (rDNA) polymorphism that was not found in parental species, but usually seen falling within the range of possible parental combinations. Yet, transgressive phenotypes that cannot be explained by normal recombination, including more rDNA clusters than expected or the occurrence of syntenic rDNAs, were also detected. Present results proved rapid genomic evolution providing the genetic novelty for species to persist. In addition, although the ultimate consequences of such apparently extensive and recurrent events remain unknown, modern genome-wide methodologies are of great promise towards answering questions concerning the causes, dynamics and impacts of hybridization.

  2. Discrimination of bacteriophage infected cells using locked nucleic acid fluorescent in situ hybridization (LNA-FISH).

    Science.gov (United States)

    Vilas Boas, Diana; Almeida, Carina; Sillankorva, Sanna; Nicolau, Ana; Azeredo, Joana; Azevedo, Nuno F

    2016-01-01

    Bacteriophage-host interaction studies in biofilm structures are still challenging due to the technical limitations of traditional methods. The aim of this study was to provide a direct fluorescence in situ hybridization (FISH) method based on locked nucleic acid (LNA) probes, which targets the phage replication phase, allowing the study of population dynamics during infection. Bacteriophages specific for two biofilm-forming bacteria, Pseudomonas aeruginosa and Acinetobacter, were selected. Four LNA probes were designed and optimized for phage-specific detection and for bacterial counterstaining. To validate the method, LNA-FISH counts were compared with the traditional plaque forming unit (PFU) technique. To visualize the progression of phage infection within a biofilm, colony-biofilms were formed and infected with bacteriophages. A good correlation (r = 0.707) was observed between LNA-FISH and PFU techniques. In biofilm structures, LNA-FISH provided a good discrimination of the infected cells and also allowed the assessment of the spatial distribution of infected and non-infected populations.

  3. CLINICAL STUDY OF CHROMOSOMAL ABNORMALITY IN MISSED ABORTION TISSUE DETECTED BY FLUORESCENCE IN SITU HYBRIDIZATION TECHNIQUE%FISH 技术检测稽留流产组织中染色体异常的临床研究

    Institute of Scientific and Technical Information of China (English)

    王春珺; 于晶峰

    2014-01-01

    目的:探讨稽留流产胎儿组织细胞中染色体异常总发生率及异常种类和各种异常的发生率。方法:采用18号、X 和 Y 染色体着丝粒探针及13、16、21、22号染色体单一序列探针,对105例稽留流产胎儿组织进行FISH 检测。结果:49.5%的稽留流产是由胎儿染色体异常引起的;染色体异常的前3位为三倍体,16号染色体三体,X 单体,分别占染色体异常总发生率的32.7%、15.4%和15.4%;高龄组(≥35岁)和非高龄组(0.05);妊娠≤12wk和妊娠>12wk者染色体异常率分别为60.9%和31.7%,差异有统计学意义(P0. 05). The detection rate of chromosomal abnormality in the cases of ≤12 gestational weeks was 60. 9%,while the detection rate of chromosomal abnormality in the cases of >12 gestational weeks was 31. 7%,there was significant difference(P<0. 05). Conclusion:Most missed abortion are caused by genetic gene defect, FISH technique can detect aborted fetuses, find chromosomal abnormality rapidly and accurately,provide data for genetic counseling of subsequent pregnancy.

  4. A New Generic Taxonomy on Hybrid Malware Detection Technique

    CERN Document Server

    Robiah, Y; Zaki, M Mohd; Shahrin, S; Faizal, M A; Marliza, R

    2009-01-01

    Malware is a type of malicious program that replicate from host machine and propagate through network. It has been considered as one type of computer attack and intrusion that can do a variety of malicious activity on a computer. This paper addresses the current trend of malware detection techniques and identifies the significant criteria in each technique to improve malware detection in Intrusion Detection System (IDS). Several existing techniques are analyzing from 48 various researches and the capability criteria of malware detection technique have been reviewed. From the analysis, a new generic taxonomy of malware detection technique have been proposed named Hybrid Malware Detection Technique (Hybrid MDT) which consists of Hybrid Signature and Anomaly detection technique and Hybrid Specification based and Anomaly detection technique to complement the weaknesses of the existing malware detection technique in detecting known and unknown attack as well as reducing false alert before and during the intrusion ...

  5. Detection of airborne Legionella while showering using liquid impingement and fluorescent in situ hybridization (FISH).

    Science.gov (United States)

    Deloge-Abarkan, Magali; Ha, Thi-Lan; Robine, Enric; Zmirou-Navier, Denis; Mathieu, Laurence

    2007-01-01

    Aerosols of water contaminated with Legionella bacteria constitute the only mode of exposure for humans. However, the prevention strategy against this pathogenic bacteria risk is managed through the survey of water contamination. No relationship linked the Legionella bacteria water concentration and their airborne abundance. Therefore, new approaches in the field of the metrological aspects of Legionella bioaerosols are required. This study was aimed at testing the main principles for bioaerosol collection (solid impaction, liquid impingement and filtration) and the in situ hybridization (FISH) method, both in laboratory and field assays, with the intention of applying such methodologies for airborne Legionella bacteria detection while showering. An aerosolization chamber was developed to generate controlled and reproducible L. pneumophila aerosols. This tool allowed the identification of the liquid impingement method as the most appropriate one for collecting airborne Legionella bacteria. The culturable fraction of airborne L. pneumophila recovered with the liquid impingement principle was 4 and 700 times higher compared to the impaction and filtration techniques, respectively. Moreover, the concentrations of airborne L. pneumophila in the impinger fluid were on average 7.0 x 10(5) FISH-cells m(-3) air with the fluorescent in situ hybridization (FISH) method versus 9.0 x 10(4) CFU m(-3) air with the culture method. These results, recorded under well-controlled conditions, were confirmed during the field experiments performed on aerosols generated by hot water showers in health institutions. This new approach may provide a more accurate characterization of aerobiocontamination by Legionella bacteria.

  6. Characterization of B chromosomes in Lilium hybrids through GISH and FISH

    NARCIS (Netherlands)

    Xie, S.L.; Marasek-Ciolakowska, A.; Ramanna, M.S.; Arens, P.F.P.; Visser, R.G.F.; Tuyl, van J.M.

    2014-01-01

    Supernumerary (B) chromosomes and small aberrant chromosomes were detected in Lilium hybrids and characterized through genomic in situ hybridization (GISH) and florescence in situ hybridization (FISH). Two small, supernumerary or B chromosomes were detected as extra chromosomes in a tetraploid plant

  7. Wind Speed Forecasting Using Hybrid Wavelet Transform—ARMA Techniques

    Directory of Open Access Journals (Sweden)

    Diksha Kaur

    2015-01-01

    Full Text Available The objective of this paper is to develop a novel wind speed forecasting technique, which produces more accurate prediction. The Wavelet Transform (WT along with the Auto Regressive Moving Average (ARMA is chosen to form a hybrid whose combination is expected to give minimum Mean Absolute Prediction Error (MAPE. A simulation study has been conducted by comparing the forecasting results using the Wavelet-ARMA with the ARMA and Artificial Neural Network (ANN-Ensemble Kalman Filter (EnKF hybrid technique to verify the effectiveness of the proposed hybrid method. Results of the proposed hybrid show significant improvements in the forecasting error.

  8. Identification of Potential Fishing Grounds Using Geospatial Technique

    Science.gov (United States)

    Abdullah, Muhammad

    2016-07-01

    Fishery resources surveys using actual sampling and data collection methods require extensive ship time and sampling time. Informative data from satellite plays a vital role in fisheries application. Satellite Remote Sensing techniques can be used to detect fish aggregation just like visual fish identification ultimately these techniques can be used to predict the potential fishing zones by measuring the parameters which affect the distribution of fishes. Remote sensing is a time saving technique to locate fishery resources along the coast. Pakistan has a continental shelf area of 50,270 km2 and coastline length of 1,120 km. The total maritime zone of Pakistan is over 30 percent of the land area. Fishery plays an important role in the national economy. The marine fisheries sector is the main component, contributing about 57 percent in terms of production. Fishery is the most important economic activity in the villages and towns along the coast, and in most of the coastal villages and settlements it is the sole source of employment and income generation. Fishing by fishermen is done on the sole basis of repeated experiments and collection of information from other fishermen. Often they are in doubt about the location of potential fishing zones. This leads to waste of time and money, adversely affecting fishermen incomes and over or under-exploitation of fishing zones. The main purpose of this study was to map potential fishing grounds by identifying various environmental parameters which impact fish aggregation along the Pakistan coastline. The primary reason of this study is the fact that the fishing communities of Pakistan's coastal regions are extremely poor and lack knowledge of the modern tools and techniques that may be incorporated to enhance their yield and thus, improve their livelihood. Using geospatial techniques in order to accurately map the potential fishing zones based on sea surface temperature (SST) and chlorophyll -a content, in conjunction with

  9. Mitochondrial Genome Variation after Hybridization and Differences in the First and Second Generation Hybrids of Bream Fishes.

    Directory of Open Access Journals (Sweden)

    Wei-Zhuo Zhang

    Full Text Available Hybridization plays an important role in fish breeding. Bream fishes contribute a lot to aquaculture in China due to their economically valuable characteristics and the present study included five bream species, Megalobrama amblycephala, Megalobrama skolkovii, Megalobrama pellegrini, Megalobrama terminalis and Parabramis pekinensis. As maternal inheritance of mitochondrial genome (mitogenome involves species specific regulation, we aimed to investigate in which way the inheritance of mitogenome is affected by hybridization in these fish species. With complete mitogenomes of 7 hybrid groups of bream species being firstly reported in the present study, a comparative analysis of 17 mitogenomes was conducted, including representatives of these 5 bream species, 6 first generation hybrids and 6 second generation hybrids. The results showed that these 17 mitogenomes shared the same gene arrangement, and had similar gene size and base composition. According to the phylogenetic analyses, all mitogenomes of the hybrids were consistent with a maternal inheritance. However, a certain number of variable sites were detected in all F1 hybrid groups compared to their female parents, especially in the group of M. terminalis (♀ × M. amblycephala (♂ (MT×MA, with a total of 86 variable sites between MT×MA and its female parent. Among the mitogenomes genes, the protein-coding gene nd5 displayed the highest variability. The number of variation sites was found to be related to phylogenetic relationship of the parents: the closer they are, the lower amount of variation sites their hybrids have. The second generation hybrids showed less mitogenome variation than that of first generation hybrids. The non-synonymous and synonymous substitution rates (dN/dS were calculated between all the hybrids with their own female parents and the results indicated that most PCGs were under negative selection.

  10. Effect of graded fingerlings on hybrid catfish food fish size distribution

    Science.gov (United States)

    It is not unusual to have both 0.5 lb and 5 lb fish harvested from a single-batch hybrid catfish production pond at the end of the growing season. When that happens, farmers may be docked for fish that are either larger or smaller than the processor’s preferred size range. This study was conducted t...

  11. Role of fluorescence in situ hybridization (FISH) in sequencing the tomato genome

    Science.gov (United States)

    Chromosomes at various stages of the cell cycle can be used for localization of DNA probes via Fluorescence in situ hybridization (FISH). While mitotic metaphase chromosomes are demonstrably too short and compact for this purpose, long pachytene chromosomes are ideal. BACs that hybridize to euchrom...

  12. Fluorescent in situ hybridization (FISH) assessment of chromosome copy number in sperm

    Energy Technology Data Exchange (ETDEWEB)

    Sheu, M.; Sigman, M.; Mark, H.F.L. [Brown Univ. School of Medicine, Providence, RI (United States)

    1994-09-01

    Approximately 15% of all recognized pregnancies end in spontaneous abortions. The overall frequency of chromosome abnormalities in spontaneous abortions is approximately 50%. Thus aneuploidy is a significant cause of fetal wastage. In addition, structural and numerical abnormalities of chromosomes can also lead to birth defects, developmental delay, mental retardation and infertility. Conventional cytogenetic analysis via GTG- and other banding techniques is a powerful tool in the elucidation of the nature of chromosomal abnormalities. Fluorescent in situ hybridization (FISH) enables detection of numerical chromosomal abnormalities, especially trisomies, in intact cells. Using FISH and commercially available biotin-labeled probes, we have initiated a prospective study to assess specific chromosome copy number of preparations of unstained smears from men referred for a male infertility evaluation as well as smears from normal control males chosen randomly from the sample of sperm donors. A total of approximately 19,000 sperm nuclei have been examined thus far. Of those suitable for analysis, 7382 (38.75%) were normal possessing one copy of chromosome 8, 155 (0.81%) were disomic, and 15 (0.079%) had more than two copies of chromosome 8. Comparisons with data available in the literature will be discussed. Work is ongoing to increase the efficiency of hybridization using both reported and previously untried pretreatment and fixation protocols. We have also initiated studies using multicolor FISH with various chromosome enumeration probes. The assay described here is a potentially powerful tool for detecting rare events such as spontaneous germ cell aneuploidy, aneuploidy detected in semen from men with carcinoma in situ of the testis and aneuploidy induced by potential environmental genotoxicants. It can also be utilized for segregation analysis and for correlating chromosome copy number with germ cell morphology.

  13. The Formation of the Polyploid Hybrids From Different Subfamily Fish Crossings and Its Evolutionary Significance

    OpenAIRE

    Liu, Shaojun; Qin, Qinbo; Xiao, Jun; Lu, Wenting; Shen, Jiamin; Li, Wei; Liu, Jifang; Duan, Wei; Zhang, Chun; Tao, De Min; Zhao, Rurong; Yan, Jinpeng; Liu, Yun

    2007-01-01

    This study provides genetic evidences at the chromosome, DNA content, DNA fragment and sequence, and morphological levels to support the successful establishment of the polyploid hybrids of red crucian carp × blunt snout bream, which belonged to a different subfamily of fish (Cyprininae subfamily and Cultrinae subfamily) in the catalog. We successfully obtained the sterile triploid hybrids and bisexual fertile tetraploid hybrids of red crucian carp (RCC) (♀) × blunt snout bream (BSB) (♂) as w...

  14. Diagnostic accuracy: theoretical models for preimplantation genetic testing of a single nucleus using the fluorescence in situ hybridization technique

    NARCIS (Netherlands)

    P.N. Scriven; P.M.M. Bossuyt

    2010-01-01

    The aim of this study was to develop and use theoretical models to investigate the accuracy of the fluorescence in situ hybridization (FISH) technique in testing a single nucleus from a preimplantation embryo without the complicating effect of mosaicism. Mathematical models were constructed for thre

  15. 荧光原位杂交(FISH)技术在转基因小鼠检测中的应用%Application of the technique of fluorescence in situ hybridization in detecting transgenic mice

    Institute of Scientific and Technical Information of China (English)

    谢建云; 邵伟娟; 潘漪清; 高诚

    2002-01-01

    制备转基因小鼠特有的DIG标记探针,应用染色体荧光原位杂交(FISH)技术,对转基因小鼠外周血细胞的遗传性状进行分析,检测其合子类型,以挑选纯合子小鼠用于保种.结果杂合型小鼠61%的细胞有一个荧光信号,而纯合型小鼠约24%的细胞有两个荧光信号,48%的细胞有一个荧光信号,野生型小鼠无荧光信号.因此染色体荧光原位杂交技术可作为检测转基因小鼠遗传特性的一种方法.

  16. The formation of the polyploid hybrids from different subfamily fish crossings and its evolutionary significance.

    Science.gov (United States)

    Liu, Shaojun; Qin, Qinbo; Xiao, Jun; Lu, Wenting; Shen, Jiamin; Li, Wei; Liu, Jifang; Duan, Wei; Zhang, Chun; Tao, Min; Zhao, Rurong; Yan, Jinpeng; Liu, Yun

    2007-06-01

    This study provides genetic evidences at the chromosome, DNA content, DNA fragment and sequence, and morphological levels to support the successful establishment of the polyploid hybrids of red crucian carp x blunt snout bream, which belonged to a different subfamily of fish (Cyprininae subfamily and Cultrinae subfamily) in the catalog. We successfully obtained the sterile triploid hybrids and bisexual fertile tetraploid hybrids of red crucian carp (RCC) (female symbol) x blunt snout bream (BSB) (male symbol) as well as their pentaploid hybrids. The triploid hybrids possessed 124 chromosomes with two sets from RCC and one set from BSB; the tetraploid hybrids had 148 chromosomes with two sets from RCC and two sets from BSB. The females of tetraploid hybrids produced unreduced tetraploid eggs that were fertilized with the haploid sperm of BSB to generate pentaploid hybrids with 172 chromosomes with three sets from BSB and two sets from RCC. The ploidy levels of triploid, tetraploid, and pentaploid hybrids were confirmed by counting chromosomal number, forming chromosomal karyotype, and measuring DNA content and erythrocyte nuclear volume. The similar and different DNA fragments were PCR amplified and sequenced in triploid, tetraploid hybrids, and their parents, indicating their molecular genetic relationship and genetic markers. In addition, this study also presents results about the phenotypes and feeding habits of polyploid hybrids and discusses the formation mechanism of the polyploid hybrids. It is the first report on the formation of the triploid, tetraploid, and pentaploid hybrids by crossing parents with a different chromosome number in vertebrates. The formation of the polyploid hybrids is potentially interesting in both evolution and fish genetic breeding.

  17. Ultra Wide Band localization and tracking hybrid technique using VRTs

    Directory of Open Access Journals (Sweden)

    Mohd Shamian Zianal

    2012-12-01

    Full Text Available This research presents hybrid radar tracking technique consisting of Time Of Arrival (TOA and Received Signal Strength (RSS techniques. This hybrid design increases efficiency, accuracy and sensitivity of radar system. The radar used in this research is multistatic radar with one transmitter and three receivers. One common drawback in RSS and TOA techniques is high level synchronization in transmitter and receivers. The hybrid design also suffers from transmitter-receiver synchronization. To overcome TX-RX synchronization problem Virtual Reference Tags (VRTs are used. These tags are virtually mapped over the surveillance area giving radar design different reference points from which it can accurately locate intruder and monitor its movements. Also four cases of different

  18. Current and historical hybridization with differential introgression among three species of cyprinid fishes (genus Cyprinella).

    Science.gov (United States)

    Broughton, Richard E; Vedala, Krishna C; Crowl, Tessa M; Ritterhouse, Lauren L

    2011-05-01

    Hybridization is common among freshwater fishes, particular among the Cyprinidae. We used two mitochondrial genes and one nuclear gene to characterize hybridization among two species pairs of Cyprinella in southwestern North America. Genalogical patterns revealed that C. lutrensis and C. venusta are currently hybridizing in several localities producing apparent F(1), F(2) and backcross generations, yet there was no evidence for introgression outside of local hybrid zones. Alternatively, mitochondrial haplotypes from C. lutrensis appear to have introgressed into a C. lepida population in the Nueces River completely replacing the native C. lepida haplotype. There was no evidence of introgression of nuclear DNA and there does not appear to be ongoing hybridization. The population of C. lepida from the nearby Frio River exhibits no evidence of hybridization with C. lutrensis. Thus, contact between C. lutrensis and C. venusta results in the formation of localized hybrid swarms, while contact between C. lutrensis and C. lepida has resulted in complete mitochondrial introgression in the Nueces River or no apparent hybridization in the Frio River. The three different outcomes of contact between these species illustrate the variable nature of interspecific reproductive interactions and provide an excellent system in which to better understand the factors influencing hybridization among freshwater fishes.

  19. CONGESTION MANAGEMENT BY OPTIMAL ALLOCATION OF FACTS CONTROLLERS USING HYBRID FISH BEE OPTIMIZATION

    Directory of Open Access Journals (Sweden)

    S. Thangalakshmi

    2014-01-01

    Full Text Available The role of Independent System Operator (ISO in the restructured power industry includes system control, capacity planning, transmission tariff and congestion management; the challenging task being minimizing the congestion. One of the popular techniques used to alleviate congestion is using Flexible AC Transmission Systems (FACTS devices. The power system generally operates near its rated capacity in deregulated market because of intensive usage of transmission grids. So, the major issues that need to be addressed are improving the voltage profile and reducing the power loss in the electrical network. Motivation: The location of FACTS devices can improve the power flow in the line, maintain the bus profile and reduce the losses. However locating the ideal location is a NP problem. This study presents a novel heuristic method to determine the types of FACTS devices and its optimal location in a power system without violating the thermal and voltage limits. Power flow sensitivity index to find the optimal location of UPFC is suggested in this study. A hybrid fish bee swarm optimization is proposed which is based on Artificial Bee Colony (ABC and Fish School Search (FSS methods. This proposed algorithm is tested based on IEEE 30 bus system and line performances are studied.

  20. Chromosome painting by GISH and multi-color FISH

    Science.gov (United States)

    Fluorescent in situ hybridization (FISH) is a powerful cytogenetic technique for identifying chromosomes and mapping specific genes and DNA sequences on individual chromosomes. Genomic in situ hybridization (GISH) and multi-color FISH (mc-FISH) represent two special types of FISH techniques. Both ...

  1. Comparison Between Fluorescent In Situ Hybridization (FISH) and ...

    African Journals Online (AJOL)

    ... were collected, for culture method and bacteria characterized by biochemical ... for 24 hours and later preserved in 70% alcohol before in situ hybridization test. ... were observed in 47 to 75% while the bacterium was isolated on culture in 7 ...

  2. A hybrid localization technique for patient tracking.

    Science.gov (United States)

    Rodionov, Denis; Kolev, George; Bushminkin, Kirill

    2013-01-01

    Nowadays numerous technologies are employed for tracking patients and assets in hospitals or nursing homes. Each of them has advantages and drawbacks. For example, WiFi localization has relatively good accuracy but cannot be used in case of power outage or in the areas with poor WiFi coverage. Magnetometer positioning or cellular network does not have such problems but they are not as accurate as localization with WiFi. This paper describes technique that simultaneously employs different localization technologies for enhancing stability and average accuracy of localization. The proposed algorithm is based on fingerprinting method paired with data fusion and prediction algorithms for estimating the object location. The core idea of the algorithm is technology fusion using error estimation methods. For testing accuracy and performance of the algorithm testing simulation environment has been implemented. Significant accuracy improvement was showed in practical scenarios.

  3. New techniques for emulsion analysis in a hybrid experiment

    Energy Technology Data Exchange (ETDEWEB)

    Kodama, K. (Aichi University of Education, Kariya 448 (Japan)); Ushida, N. (Aichi University of Education, Kariya 448 (Japan)); Mokhtarani, A. (University of California (Davis), Davis, CA 95616 (United States)); Paolone, V.S. (University of California (Davis), Davis, CA 95616 (United States)); Volk, J.T. (University of California (Davis), Davis, CA 95616 (United States)); Wilcox, J.O. (University of California (Davis), Davis, CA 95616 (United States)); Yager, P.M. (University of California (Davis), Davis, CA 95616 (United States)); Edelstein, R.M. (Carnegie-Mellon University, Pittsburgh, PA 15213 (United States)); Freyberger, A.P. (Carnegie-Mellon University, Pittsburgh, PA 15213 (United States)); Gibaut, D.B. (Carnegie-Mellon University, Pittsburgh, PA 15213 (United States)); Lipton, R.J. (Carnegie-Mellon University, Pittsburgh, PA 15213 (United States)); Nichols, W.R. (Carnegie-Mellon University, Pittsburgh, PA 15213 (United States)); Potter, D.M. (Carnegie-Mellon Univers

    1994-08-01

    A new method, called graphic scanning, was developed by the Nagoya University Group for emulsion analysis in a hybrid experiment. This method enhances both speed and reliability of emulsion analysis. Details of the application of this technique to the analysis of Fermilab experiment E653 are described. ((orig.))

  4. A Hybridized Centroid Technique for 3D Molodensky-Badekas ...

    African Journals Online (AJOL)

    Richannan

    two new hybrid centroid techniques known as the harmonic-quadratic mean and arithmetic- ... was subsequently involved in triangulation nets with other trigonometric ..... The functional relation that is used to compute the TLS solution is given by ..... mathematical models is an approximation of reality, hence it is worth noting ...

  5. Trypanosomiasis in hybrid catfish (Clarias macrocephalus x Clarias gariepinus and other freshwater fishes

    Directory of Open Access Journals (Sweden)

    Promkhunthong, W.

    2005-02-01

    Full Text Available The infestation of Trypanosoma sp. (Kinetoplastida in the hybrid catfish and some fresh water fish was studied showing such parasitic infestation was specific only in catfish. The virulence as determined by LD50 for 5 days was 2.28x1010 cell in a fish sample. The parasitic infestation caused hematological changes by the reduction of red as well as white blood cells. The reductions were highly significant as compared to the healthy sample (p<0.05 as noted by the red and white blood cell count which dropped from 2.14±0.48x106 to 1.62±0.27x106 cells/ml and from 1.45±3.76x105 to 2.42±0.78x104 cells/ml blood in the infested samples, respectively. Similar trend was noted for hemoglobin and hematocrit which dropped significantly (p<0.05. The hemoglobin in healthy fish is 7.075±0.929g/100g, which dropped to 6.268±0.697g/100g in the samples with infestation. The percentage of hematocrit in healthy sample is 25.275±3.318%, which dropped to 21.722±3.068% in the samples with infestation. The reverse trend was recognized for serum protein andleukocrit which increased in the samples with Trypanosoma sp. infestation. The density gradient centrifugation technique was employed in the isolation of parasites in which 50% Percoll solution in 0.85% final preparation of saline solution was capable of removing Trypanosoma sp. from the blood. The study of antibody levels in serum showed that the infested hybrid catfish could develop the antibody which reached a peak 14 days after the infestation. Trypanosoma sp. was unable to cause histological changes in the tissues of gill, liver, kidney, spleen, stomach and intestine. Minor inflammations were observed, even the cases that large number of parasites were found in the tissues, blood streams and sinuses. Marked reductions were recorded for mature red blood cells while there were the formation of immature red blood and phagocytotic cells at higher rates as compared to the healthy individual.

  6. Characterization of the Arachis (Leguminosae) D genome using fluorescence in situ hybridization (FISH) chromosome markers and total genome DNA hybridization

    OpenAIRE

    Germán Robledo; Guillermo Seijo

    2008-01-01

    Chromosome markers were developed for Arachis glandulifera using fluorescence in situ hybridization (FISH) of the 5S and 45S rRNA genes and heterochromatic 4'-6-diamidino-2-phenylindole (DAPI) positive bands. We used chromosome landmarks identified by these markers to construct the first Arachis species ideogram in which all the homologous chromosomes were precisely identified. The comparison of this ideogram with those published for other Arachis species revealed very poor homeologies with a...

  7. Bonding techniques for hybrid active pixel sensors (HAPS)

    Science.gov (United States)

    Bigas, M.; Cabruja, E.; Lozano, M.

    2007-05-01

    A hybrid active pixel sensor (HAPS) consists of an array of sensing elements which is connected to an electronic read-out unit. The most used way to connect these two different devices is bump bonding. This interconnection technique is very suitable for these systems because it allows a very fine pitch and a high number of I/Os. However, there are other interconnection techniques available such as direct bonding. This paper, as a continuation of a review [M. Lozano, E. Cabruja, A. Collado, J. Santander, M. Ullan, Nucl. Instr. and Meth. A 473 (1-2) (2001) 95-101] published in 2001, presents an update of the different advanced bonding techniques available for manufacturing a hybrid active pixel detector.

  8. Detection of Inter-chromosomal Stable Aberrations by Multiple Fluorescence In Situ Hybridization (mFISH) and Spectral Karyotyping (SKY) in Irradiated Mice.

    Science.gov (United States)

    Pathak, Rupak; Koturbash, Igor; Hauer-Jensen, Martin

    2017-01-11

    Ionizing radiation (IR) induces numerous stable and unstable chromosomal aberrations. Unstable aberrations, where chromosome morphology is substantially compromised, can easily be identified by conventional chromosome staining techniques. However, detection of stable aberrations, which involve exchange or translocation of genetic materials without considerable modification in the chromosome morphology, requires sophisticated chromosome painting techniques that rely on in situ hybridization of fluorescently labeled DNA probes, a chromosome painting technique popularly known as fluorescence in situ hybridization (FISH). FISH probes can be specific for whole chromosome/s or precise sub-region on chromosome/s. The method not only allows visualization of stable aberrations, but it can also allow detection of the chromosome/s or specific DNA sequence/s involved in a particular aberration formation. A variety of chromosome painting techniques are available in cytogenetics; here two highly sensitive methods, multiple fluorescence in situ hybridization (mFISH) and spectral karyotyping (SKY), are discussed to identify inter-chromosomal stable aberrations that form in the bone marrow cells of mice after exposure to total body irradiation. Although both techniques rely on fluorescent labeled DNA probes, the method of detection and the process of image acquisition of the fluorescent signals are different. These two techniques have been used in various research areas, such as radiation biology, cancer cytogenetics, retrospective radiation biodosimetry, clinical cytogenetics, evolutionary cytogenetics, and comparative cytogenetics.

  9. Inconsistent phylogeographic pattern between a sperm dependent fish and its host: in situ hybridization vs dispersal.

    Science.gov (United States)

    Vergilino, Roland; Leung, Christelle; Angers, Bernard

    2016-09-06

    Co-dispersal of sperm-dependent hybrids and their sexual relatives is expected to result in consistent spatial patterns between assemblages of hybrids and genetic structure of parental species. However, local hybridization events may blur this signal as assemblages could be organized under different connectivity constraints. This study aims at testing the hypothesis of local hybridization events by comparing the assemblage of hybrid fish Chrosomus eos-neogaeus to the genetic diversity of one of its parental species, Chrosomus eos. An extensive survey performed on a total of 132 sites located in two regions of Southern Quebec (West-Qc and East-Qc) revealed a distinct organization of hybrid lineages. One of the six hybrid lineages detected in West-Qc is widespread throughout this region resulting in a low α-diversity (1.38) and β-diversity (4.35). On the other hand, 36 hybrid lineages were detected in East-Qc and displayed narrow geographic distributions leading to a high α-diversity (2.30) and β-diversity (15.68). In addition, the C. eos multilocus haplotype of several of these hybrids is assigned to their respective sympatric C. eos population. Finally, contrasting with hybrids, the paternal species C. eos displayed a higher ρST in West-Qc (0.2300) than in East-Qc (0.0734). The unusually high diversity of hybrid lineages in East-Qc as well as the spatial organization and the close genetic relationship with C. eos sympatric populations support the hypothesis that multiple hybridization events occurred in situ. These findings coupled to the near absence of the maternal species Chrosomus neogeaus suggest that the decline of this species could be the trigger event at the origin of the high rates of spontaneous hybridization in this region.

  10. Behavioural isolation may facilitate homoploid hybrid speciation in cichlid fish

    NARCIS (Netherlands)

    Selz, O. M.; Thommen, R.; Maan, M. E.; Seehausen, O.

    Hybrid speciation is constrained by the homogenizing effects of gene flow from the parental species. In the absence of post-mating isolation due to structural changes in the genome, or temporal or spatial premating isolation, another form of reproductive isolation would be needed for homoploid

  11. Water quality in hybrid catfish ponds after partial fish harvest

    Science.gov (United States)

    Intensification of United States catfish aquaculture involves hybrid catfish ('channel catfish Ictalurus punctatus x ' blue catfish I. furcatus) grown in ponds with abundant aeration and high feeding rates. High feeding rates cause water quality deterioration because most of the nitrogen, phosphorus...

  12. Behavioural isolation may facilitate homoploid hybrid speciation in cichlid fish

    NARCIS (Netherlands)

    Selz, O. M.; Thommen, R.; Maan, M. E.; Seehausen, O.

    2014-01-01

    Hybrid speciation is constrained by the homogenizing effects of gene flow from the parental species. In the absence of post-mating isolation due to structural changes in the genome, or temporal or spatial premating isolation, another form of reproductive isolation would be needed for homoploid hybri

  13. Telomere analysis of platyhelminths and acanthocephalans by FISH and Southern hybridization.

    Science.gov (United States)

    Bombarová, Marta; Vítková, Magda; Spakulová, Marta; Koubková, Bozena

    2009-11-01

    We examined the composition of telomeres in chromosomes of parasitic worms, representatives of the flatworm groups Monogenea and Cestoda (Platyhelminthes), and thorny-headed worms (Syndermata: Acanthocephala) by fluorescence in situ hybridization (FISH) with different telomeric repeat probes. Our results show that the (TTAGGG)n sequence, supposed to be the ancestral telomeric repeat motif of Metazoa, is conserved in Monogenea (Paradiplozoon homoion) and Cestoda (Caryophyllaeus laticeps, Caryophyllaeides fennica, and Nippotaenia mogurndae) but not in Acanthocephala (Pomphorhynchus laevis and Pomphorhynchus tereticollis). In the Pomphorhynchus species, no hybridization signals were obtained with the "nematode" (TTAGGC)n, "arthropod" (TTAGG)n, and bdelloid (TGTGGG)n telomeric probes using FISH with their chromosomes and Southern hybridization with P. laevis DNA. Therefore, we suggest that parasitic Acanthocephala have evolved yet unknown telomeric repeat motifs or different mechanisms of telomere maintenance.

  14. Fluorescence in situ hybridizations (FISH) for the localization of viruses and endosymbiotic bacteria in plant and insect tissues.

    Science.gov (United States)

    Kliot, Adi; Kontsedalov, Svetlana; Lebedev, Galina; Brumin, Marina; Cathrin, Pakkianathan Britto; Marubayashi, Julio Massaharu; Skaljac, Marisa; Belausov, Eduard; Czosnek, Henryk; Ghanim, Murad

    2014-02-24

    Fluorescence in situ hybridization (FISH) is a name given to a variety of techniques commonly used for visualizing gene transcripts in eukaryotic cells and can be further modified to visualize other components in the cell such as infection with viruses and bacteria. Spatial localization and visualization of viruses and bacteria during the infection process is an essential step that complements expression profiling experiments such as microarrays and RNAseq in response to different stimuli. Understanding the spatiotemporal infections with these agents complements biological experiments aimed at understanding their interaction with cellular components. Several techniques for visualizing viruses and bacteria such as reporter gene systems or immunohistochemical methods are time-consuming, and some are limited to work with model organisms and involve complex methodologies. FISH that targets RNA or DNA species in the cell is a relatively easy and fast method for studying spatiotemporal localization of genes and for diagnostic purposes. This method can be robust and relatively easy to implement when the protocols employ short hybridizing, commercially-purchased probes, which are not expensive. This is particularly robust when sample preparation, fixation, hybridization, and microscopic visualization do not involve complex steps. Here we describe a protocol for localization of bacteria and viruses in insect and plant tissues. The method is based on simple preparation, fixation, and hybridization of insect whole mounts and dissected organs or hand-made plant sections, with 20 base pairs short DNA probes conjugated to fluorescent dyes on their 5' or 3' ends. This protocol has been successfully applied to a number of insect and plant tissues, and can be used to analyze expression of mRNAs or other RNA or DNA species in the cell.

  15. Wind Speed Forecasting Using Hybrid Wavelet Transform—ARMA Techniques

    OpenAIRE

    Diksha Kaur; Tek Tjing Lie; Nirmal K. C. Nair; Brice Vallès

    2015-01-01

    The objective of this paper is to develop a novel wind speed forecasting technique, which produces more accurate prediction. The Wavelet Transform (WT) along with the Auto Regressive Moving Average (ARMA) is chosen to form a hybrid whose combination is expected to give minimum Mean Absolute Prediction Error (MAPE). A simulation study has been conducted by comparing the forecasting results using the Wavelet-ARMA with the ARMA and Artificial Neural Network (ANN)-Ensemble Kalman Filter (EnKF) hy...

  16. Ecophysiological Analysis of Microorganisms in Complex Microbial Systems by Combination of Fluorescence In Situ Hybridization with Extracellular Staining Techniques

    Science.gov (United States)

    Nielsen, Jeppe Lund; Kragelund, Caroline; Nielsen, Per Halkjær

    Ecophysiological analysis and functions of single cells in complex microbial systems can be examined by simple combinations of Fluorescence in situ hybridization (FISH) for identification with various staining techniques targeting functional phenotypes. In this chapter, we describe methods and protocols optimized for the study of extracellular enzymes, surface hydrophobicity and specific surface structures. Although primarily applied to the study of microbes in wastewater treatment (activated sludge and biofilms), the methods may also be used with minor modifications in several other ecosystems.

  17. Clinical Application of Fluorescence in Situ Hybridization (FISH) to Detect HER-2 Gene in Breast Cancer

    Institute of Scientific and Technical Information of China (English)

    Maurie Buehler; Ellie Guardino; Jung Sik Park; Eun Jeong Jang

    2014-01-01

    Objective: To investigate the clinical application of the detection of HER-2 gene by lfuorescence in situ hybridization (FISH) in breast cancer and the correlation between HER-2 gene ampliifcation and clinicopathology of breast cancer. Methods:Parafifn-embedded breast inifltrating ductal carcinoma from 48 patients were detected by FISH and immunohistochemistry (IHC) respectively for comparing the results of two methods. Results: HER-2 protein expressions were classified into three groups (3+/2+/1+ or 0) and the positive rates of HER-2 gene ampliifcation by FISH were 77.8%, 57.1% and 10.5%, respectively. Of the 29 cases with positive axillary lymph node, 12 were with HER-2 gene ampliifcation (P0.05). Conclusion:The false positive and negative rates are higher in HER-2 protein expression by IHC. Compared with IHC, FISH, being more effective and precise, can be applied extensively in clinic. HER-2 gene ampliifcation is concerned with axillary nodes metastases.

  18. Early Oscillation Detection Technique for Hybrid DC/DC Converters

    Science.gov (United States)

    Wang, Bright L.

    2011-01-01

    Oscillation or instability is a situation that must be avoided for reliable hybrid DC/DC converters. A real-time electronics measurement technique was developed to detect catastrophic oscillations at early stages for hybrid DC/DC converters. It is capable of identifying low-level oscillation and determining the degree of the oscillation at a unique frequency for every individual model of the converters without disturbing their normal operations. This technique is specially developed for space-used hybrid DC/DC converters, but it is also suitable for most of commercial and military switching-mode power supplies. This is a weak-electronic-signal detection technique to detect hybrid DC/DC converter oscillation presented as a specific noise signal at power input pins. It is based on principles of feedback control loop oscillation and RF signal modulations, and is realized by using signal power spectral analysis. On the power spectrum, a channel power amplitude at characteristic frequency (CPcf) and a channel power amplitude at switching frequency (CPsw) are chosen as oscillation level indicators. If the converter is stable, the CPcf is a very small pulse and the CPsw is a larger, clear, single pulse. At early stage of oscillation, the CPcf increases to a certain level and the CPsw shows a small pair of sideband pulses around it. If the converter oscillates, the CPcf reaches to a higher level and the CPsw shows more high-level sideband pulses. A comprehensive stability index (CSI) is adopted as a quantitative measure to accurately assign a degree of stability to a specific DC/DC converter. The CSI is a ratio of normal and abnormal power spectral density, and can be calculated using specified and measured CPcf and CPsw data. The novel and unique feature of this technique is the use of power channel amplitudes at characteristic frequency and switching frequency to evaluate stability and identify oscillations at an early stage without interfering with a DC/DC converter s

  19. Ocean warming, a rapid distributional shift, and the hybridization of a coastal fish species.

    Science.gov (United States)

    Potts, Warren M; Henriques, Romina; Santos, Carmen V; Munnik, Kate; Ansorge, Isabelle; Dufois, Francois; Booth, Anthony J; Kirchner, Carola; Sauer, Warwick H H; Shaw, Paul W

    2014-09-01

    Despite increasing awareness of large-scale climate-driven distribution shifts in the marine environment, no study has linked rapid ocean warming to a shift in distribution and consequent hybridization of a marine fish species. This study describes rapid warming (0.8 °C per decade) in the coastal waters of the Angola-Benguela Frontal Zone over the last three decades and a concomitant shift by a temperature sensitive coastal fish species (Argyrosomus coronus) southward from Angola into Namibia. In this context, rapid shifts in distribution across Economic Exclusive Zones will complicate the management of fishes, particularly when there is a lack of congruence in the fisheries policy between nations. Evidence for recent hybridization between A. coronus and a congener, A. inodorus, indicate that the rapid shift in distribution of A. coronus has placed adults of the two species in contact during their spawning events. Ocean warming may therefore revert established species isolation mechanisms and alter the evolutionary history of fishes. While the consequences of the hybridization on the production of the resource remain unclear, this will most likely introduce additional layers of complexity to their management.

  20. Prediction of melting temperatures in fluorescence in situ hybridization (FISH) procedures using thermodynamic models.

    Science.gov (United States)

    Fontenete, Sílvia; Guimarães, Nuno; Wengel, Jesper; Azevedo, Nuno Filipe

    2016-01-01

    The thermodynamics and kinetics of DNA hybridization, i.e. the process of self-assembly of one, two or more complementary nucleic acid strands, has been studied for many years. The appearance of the nearest-neighbor model led to several theoretical and experimental papers on DNA thermodynamics that provide reasonably accurate thermodynamic information on nucleic acid duplexes and allow estimation of the melting temperature. Because there are no thermodynamic models specifically developed to predict the hybridization temperature of a probe used in a fluorescence in situ hybridization (FISH) procedure, the melting temperature is used as a reference, together with corrections for certain compounds that are used during FISH. However, the quantitative relation between melting and experimental FISH temperatures is poorly described. In this review, various models used to predict the melting temperature for rRNA targets, for DNA oligonucleotides and for nucleic acid mimics (chemically modified oligonucleotides), will be addressed in detail, together with a critical assessment of how this information should be used in FISH.

  1. Detection of single copy sequences using BAC-FISH and C-PRINS techniques in sunflower chromosomes.

    Science.gov (United States)

    Talia, Paola; Greizerstein, Eduardo J; Hopp, H Esteban; Paniego, Norma; Poggio, Lidia; Heinz, Ruth A

    2011-04-01

    Bacterial artificial chromosome-fluorescence in situ hybridization (BAC-FISH) and cycling-primed in situ labeling (C-PRINS) techniques were evaluated for integration of physical and genetic maps of sunflower (Helianthus annuus L.). Single-site SSR markers were selected from three linkage groups of a high-density sunflower genetic map. This selection was based on previously identified QTL associated to S. sclerotiorum. These markers were used to select BACs contaning single copy sequences for BAC-FISH aplication. Blocking of highly dispersed repetitive sunflower sequences reduced unspecific hybridization, and allowed the detection of specific signals for BACs containing SSR markers HA4222 and HA2600, anchored to LG 16 and LG 10, respectively. Single-site FISH signal detection was optimized by adjusting the relative quantity and quality of unlabelled repetitive sequences present in the blocking DNA. The SSR marker ORS1247 anchored to the LG 17 was detected by C-PRINS, which yielded fluorescence signals that were specific and intense. This progress in localizing single-copy sequences using BAC-FISH and indirect C-PRINS strategies in sunflower will facilitate the integration of genetic and physical maps, allowing the identification of chromosomes containing key genes and/or QTL associated to agronomic important traits in sunflower.

  2. Characterization of the Arachis (Leguminosae D genome using fluorescence in situ hybridization (FISH chromosome markers and total genome DNA hybridization

    Directory of Open Access Journals (Sweden)

    Germán Robledo

    2008-01-01

    Full Text Available Chromosome markers were developed for Arachis glandulifera using fluorescence in situ hybridization (FISH of the 5S and 45S rRNA genes and heterochromatic 4'-6-diamidino-2-phenylindole (DAPI positive bands. We used chromosome landmarks identified by these markers to construct the first Arachis species ideogram in which all the homologous chromosomes were precisely identified. The comparison of this ideogram with those published for other Arachis species revealed very poor homeologies with all A and B genome taxa, supporting the special genome constitution (D genome of A. glandulifera. Genomic affinities were further investigated by dot blot hybridization of biotinylated A. glandulifera total DNA to DNA from several Arachis species, the results indicating that the D genome is positioned between the A and B genomes.

  3. Analysis of Single-cell Gene Transcription by RNA Fluorescent In Situ Hybridization (FISH)

    DEFF Research Database (Denmark)

    Ronander, Elena; Bengtsson, Dominique C; Joergensen, Louise;

    2012-01-01

    and the consequence of differential binding on the clinical outcome of P. falciparum infections. Recently, the mutually exclusive transcription paradigm has been called into doubt by transcription assays based on individual P. falciparum transcript identification in single infected erythrocytic cells using RNA...... fluorescent in situ hybridization (FISH) analysis of var gene transcription by the parasite in individual nuclei of P. falciparum IE(1). Here, we present a detailed protocol for carrying out the RNA-FISH methodology for analysis of var gene transcription in single-nuclei of P. falciparum infected human...... erythrocytes. The method is based on the use of digoxigenin- and biotin- labeled antisense RNA probes using the TSA Plus Fluorescence Palette System(2) (Perkin Elmer), microscopic analyses and freshly selected P. falciparum IE. The in situ hybridization method can be used to monitor transcription...

  4. Design of Hybrid Solar and Wind Energy Harvester for Fishing Boat

    Science.gov (United States)

    Banjarnahor, D. A.; Hanifan, M.; Budi, E. M.

    2017-07-01

    In southern beach of West Java, Indonesia, there are many villagers live as fishermen. They use small boats for fishing, in one to three days. Therefore, they need a fish preservation system. Fortunately, the area has high potential of solar and wind energy. This paper presents the design of a hybrid solar and wind energy harvester to power a refrigerator in the fishing boat. The refrigerator should keep the fish in 2 - 4 °C. The energy needed is 720 Wh daily. In the area, the daily average wind velocity is 4.27 m/s and the sun irradiation is 672 W/m2. The design combined two 100W solar panels and a 300W wind turbine. The testing showed that the solar panels can harvest 815 - 817 Wh of energy, while the wind turbine can harvest 43 - 62 Wh of energy daily. Therefore, the system can fulfil the energy requirement in fishing boat, although the solar panels were more dominant. To install the wind turbine on the fishing-boat, a computational design had been conducted. The boat hydrostatic dimension was measured to determine its stability condition. To reach a stable equilibrium condition, the wind turbine should be installed no more than 1.7 m of height.

  5. Resonant frequency calculations using a hybrid perturbation-Galerkin technique

    Science.gov (United States)

    Geer, James F.; Andersen, Carl M.

    1991-01-01

    A two-step hybrid perturbation Galerkin technique is applied to the problem of determining the resonant frequencies of one or several degrees of freedom nonlinear systems involving a parameter. In one step, the Lindstedt-Poincare method is used to determine perturbation solutions which are formally valid about one or more special values of the parameter (e.g., for large or small values of the parameter). In step two, a subset of the perturbation coordinate functions determined in step one is used in Galerkin type approximation. The technique is illustrated for several one degree of freedom systems, including the Duffing and van der Pol oscillators, as well as for the compound pendulum. For all of the examples considered, it is shown that the frequencies obtained by the hybrid technique using only a few terms from the perturbation solutions are significantly more accurate than the perturbation results on which they are based, and they compare very well with frequencies obtained by purely numerical methods.

  6. Hybrid Technique of Lamellar Keratoplasty (DMEK-S

    Directory of Open Access Journals (Sweden)

    Pavel Studeny

    2013-01-01

    Full Text Available Purpose: To evaluate the outcomes of the hybrid technique of posterior lamellar keratoplasty (DMEK-S. Materials and Methods: 71 eyes of 55 patients enrolled in a single-center study underwent posterior lamellar keratoplasty with a hybrid lamella DMEK-S implanted using a solution implantation technique, owing to endothelial dysfunction. The outcome measures studied were visual acuity and endothelial cell density. Results: The rate of endothelial cell loss caused by surgery was 43.8%. During followups, we observed the stabilization of postoperative findings, or at minimum a very low rate of corneal endothelial cell loss. The UCDVA and BCDVA dramatically improved postoperatively. The rebubbling rate in our group of patients was 61.9%. We replaced the lamella due to its failure or malfunction in 17 patients (23.9%. Conclusion: In summary, DMEK-S combines the advantages of DSEK/DSAEK and DMEK. The central zone of bare Descemet’s membrane and endothelium allows for very good visual outcomes, and the peripheral rim allows for better manipulation of the lamella during implantation. It is an effective method of treating the endothelial dysfunction of various etiologies, but the high complication rate needs to be addressed before widespread implementation of the technique in the future.

  7. Detection of VHSV IVb within the gonads of Great Lakes fish using in situ hybridization.

    Science.gov (United States)

    Al-Hussinee, L; Lumsden, J S

    2011-05-24

    Viral haemorrhagic septicaemia virus (VHSV) genotype IVb was recently detected as the cause of numerous mortality events in Great Lakes fish. In situ hybridization was used to examine the gonads from 13 fish, including freshwater drum Aplodinotus grunniens and muskellunge Esox masquinongy that were infected naturally, as well as rainbow trout Oncorhynchus mykiss and fathead minnows Pimphales promelas, which were experimentally infected. Although the ovaries and testes of fish infected by VHSV IVb had few lesions, viral RNA was present in the ovaries of the rainbow trout and fathead minnow and was abundant in the gonads of muskellunge and in the ovaries of freshwater drum. Viral RNA was present mainly surrounding yolk vacuoles/granules or adjacent to the germinal vesicle, with lesser amounts found within the germinal vesicle, in the mesovarium and/or tunica albuginea and blood vessels of the ovary. Viral RNA was also found in and surrounding primary and secondary spermatocytes of the muskellunge.

  8. Detection of integrated herpesvirus genomes by fluorescence in situ hybridization (FISH).

    Science.gov (United States)

    Kaufer, Benedikt B

    2013-01-01

    Fluorescence in situ hybridization (FISH) is widely used to visualize nucleotide sequences in interphase cells or on metaphase chromosomes using specific probes that are complementary to the respective targets. Besides its broad application in cytogenetics and cancer research, FISH facilitates the localization of virus genomes in infected cells. Some herpesviruses, including human herpesvirus 6 (HHV-6) and Marek's disease virus (MDV), have been shown to integrate their genetic material into host chromosomes, which allows transmission of HHV-6 via the germ line and is required for efficient MDV-induced tumor formation. We describe here the detection by FISH of integrated herpesvirus genomes in metaphase chromosomes and interphase nuclei of herpesvirus-infected cells.

  9. Risk stratification of plasma cell neoplasm: insights from plasma cell-specific cytoplasmic immunoglobulin fluorescence in situ hybridization (cIg FISH) vs. conventional FISH.

    Science.gov (United States)

    Dong, Henry; Yang, Hai-Su; Jagannath, Sundar; Stephenson, Christine F; Brenholz, Pauline; Mazumder, Amitabha; Chari, Ajai

    2012-10-01

    We directly compared the results of routine fluorescence in situ hybridization (FISH) and plasma cell-specific cytoplasmic immunoglobulin (cIg) FISH from 75 paired samples for myeloma risk stratification. CIg FISH improves test specificity and sensitivity and tends to eliminate borderline results. It proves that most plasma cells (PCs) consistently carry the abnormality in myelomas with an IGH translocation, whereas routine FISH detects these cells only at variably low levels. Routine cytogenetic analysis of plasma cell neoplasms (PCNs) has a low sensitivity. Conventional fluorescence in situ hybridization (FISH) is not plasma cell (PC) specific and results are diluted by other cells in the sample. Although PC-specific FISH testing has been recommended for multiple myeloma (MM) risk stratification, eg, by combining cytoplasmic immunoglobulin (cIg) staining with FISH, the benefits of cIg FISH have never been directly demonstrated in a controlled study. Seventy-five samples from patients with PCNs were analyzed by concomitant conventional FISH and cIg FISH with probes for t(4;14), t(11;14), t(14;16), -13, 17p-, and +3. The results were compared for their reliability, specificity, and consistency. Apart from marginally improving detection threshold in samples with low PC burden, cIg FISH identified more abnormal cases (50 vs. 47 cases) and more chromosome abnormalities (113 vs. 103 events) than did conventional FISH. It differentiated del(13q) in myelodysplasia from MM. Remarkably, cIg FISH consistently identified a high percentage of abnormal PCs in all cases. It detected IGH translocation in 78% to 100% of PCs in all but 2 positive cases, whereas conventional FISH detected 0% to 46% in these cases (median, 91% vs. 9%). The abnormal cells found in patients with 17p- were 19% to 96% by cIg FISH vs. 0% to 13% by conventional FISH (median, 54% vs. 9%). Cases with insufficient PCs for cIg FISH had only normal conventional FISH results. CIg FISH improves reliability of

  10. Marine survey techniques: A prerequisite for exploratory fishing

    Digital Repository Service at National Institute of Oceanography (India)

    Pathak, M.C.; Kotnala, K.L.

    stream_size 10 stream_content_type text/plain stream_name Trg_Course_Fish_Related_Oceanogr_1991_17.pdf.txt stream_source_info Trg_Course_Fish_Related_Oceanogr_1991_17.pdf.txt Content-Encoding ISO-8859-1 Content-Type text...

  11. DETERMINATION OF HISTAMINE IN FISH USING ELISA TECHNIQUE

    NARCIS (Netherlands)

    KRUGER, C; SEWING, U; STENGEL, G; KEMA, [No Value; WESTERMANN, J; MANZ, B

    1995-01-01

    The analysis of histamine in fish and fish products via competitive ELISA is described. The advantages of this method are easy sample preparation and handling, screening capabilities, and low costs. Automation enables the performance of the assay with higher series of samples. The Histamine-ELISA is

  12. A simple protocol for attenuating the auto-fluorescence of cyanobacteria for optimized fluorescence in situ hybridization (FISH) imaging.

    Science.gov (United States)

    Zeller, Perrine; Ploux, Olivier; Méjean, Annick

    2016-03-01

    Cyanobacteria contain pigments, which generate auto-fluorescence that interferes with fluorescence in situ hybridization (FISH) imaging of cyanobacteria. We describe simple chemical treatments using CuSO4 or H2O2 that significantly reduce the auto-fluorescence of Microcystis strains. These protocols were successfully applied in FISH experiments using 16S rRNA specific probes and filamentous cyanobacteria.

  13. A hybrid sequential deposition fabrication technique for micro fuel cells

    Science.gov (United States)

    Stanley, Kevin G.; Czyzewska, Eva K.; Vanderhoek, Tom P. K.; Fan, Lilian L. Y.; Abel, Keith A.; Wu, Q. M. Jonathan; Parameswaran, M. Ash

    2005-10-01

    Micro fuel cell systems have elicited significant interest due to their promise for instantly rechargeable, longer duration and portable power. Most micro fuel cell systems are either built as miniaturized plate-and-frame or silicon-based microelectromechanical systems (MEMS). Plate-and-frame systems are difficult to fabricate smaller than 20 cm3. Existing micro fuel cell designs cannot meet the cost, scale and power requirements of some portable power markets. Traditional MEMS scaling advantages do not apply to fuel cells because the minimum area for the fuel cell is fixed by the catalyst area required for a given power output, and minimum volume set by mass transport limitations. We have developed a new hybrid technique that borrows from both micro and macro machining techniques to create fuel cells in the 1-20 cm3 range, suitable for cell phones, PDAs and smaller devices.

  14. An Adaptive Hybrid Multiprocessor technique for bioinformatics sequence alignment

    KAUST Repository

    Bonny, Talal

    2012-07-28

    Sequence alignment algorithms such as the Smith-Waterman algorithm are among the most important applications in the development of bioinformatics. Sequence alignment algorithms must process large amounts of data which may take a long time. Here, we introduce our Adaptive Hybrid Multiprocessor technique to accelerate the implementation of the Smith-Waterman algorithm. Our technique utilizes both the graphics processing unit (GPU) and the central processing unit (CPU). It adapts to the implementation according to the number of CPUs given as input by efficiently distributing the workload between the processing units. Using existing resources (GPU and CPU) in an efficient way is a novel approach. The peak performance achieved for the platforms GPU + CPU, GPU + 2CPUs, and GPU + 3CPUs is 10.4 GCUPS, 13.7 GCUPS, and 18.6 GCUPS, respectively (with the query length of 511 amino acid). © 2010 IEEE.

  15. Recent advances in methods and techniques for freshness quality determination and evaluation of fish and fish fillets: a review.

    Science.gov (United States)

    Cheng, Jun-Hu; Sun, Da-Wen; Zeng, Xin-An; Liu, Dan

    2015-01-01

    The freshness quality of fish plays an important role in human health and the acceptance of consumers as well as in international fishery trade. Recently, with food safety becoming a critical issue of great concern in the world, determination and evaluation of fish freshness is much more significant in research and development. This review renovates and concentrates recent advances of evaluating methods for fish freshness as affected by preharvest and postharvest factors and highlights the determination methods for fish freshness including sensory evaluation, microbial inspection, chemical measurements of moisture content, volatile compounds, protein changes, lipid oxidation, and adenosine triphosphate (ATP) decomposition (K value), physical measurements, and foreign material contamination detection. Moreover, the advantages and disadvantages of these methods and techniques are compared and discussed and some viewpoints about the current work and future trends are also presented.

  16. The complete mitochondrial genome of Channa argus, Channa maculata and hybrid snakehead fish [Channa maculata (♀) × Channa argus (♂)].

    Science.gov (United States)

    Zhu, Shu-Ren; Ma, Ke-Yi; Xing, Zhi-Jun; Xie, Nan; Wang, Yu-Xi; Wang, Qun; Li, Jia-Le

    2013-06-01

    We sequenced and characterized the complete mitochondrial genome of Channa argus, Channa maculata and their hybrid [C. maculata (♀) and C. argus (♂)]. All the three mitochondrial genomes contained the typical complement of 13 protein-coding genes, 22 transfer RNAs (tRNAs), 2 ribosomal RNAs (rRNAs) and 1 control region. The entire mitochondrial DNA (mtDNA) molecule of C. maculata was 16,559 bp long while the complete mtDNA molecule of C. argus and hybrid snakehead fish was 16,558 bp long. This is the first report on the complete mitogenome sequence of C. maculata and hybrid snakehead fish.

  17. Automation of ALK gene rearrangement testing with fluorescence in situ hybridization (FISH): a feasibility study.

    Science.gov (United States)

    Zwaenepoel, Karen; Merkle, Dennis; Cabillic, Florian; Berg, Erica; Belaud-Rotureau, Marc-Antoine; Grazioli, Vittorio; Herelle, Olga; Hummel, Michael; Le Calve, Michele; Lenze, Dido; Mende, Stefanie; Pauwels, Patrick; Quilichini, Benoit; Repetti, Elena

    2015-02-01

    In the past several years we have observed a significant increase in our understanding of molecular mechanisms that drive lung cancer. Specifically in the non-small cell lung cancer sub-types, ALK gene rearrangements represent a sub-group of tumors that are targetable by the tyrosine kinase inhibitor Crizotinib, resulting in significant reductions in tumor burden. Phase II and III clinical trials were performed using an ALK break-apart FISH probe kit, making FISH the gold standard for identifying ALK rearrangements in patients. FISH is often considered a labor and cost intensive molecular technique, and in this study we aimed to demonstrate feasibility for automation of ALK FISH testing, to improve laboratory workflow and ease of testing. This involved automation of the pre-treatment steps of the ALK assay using various protocols on the VP 2000 instrument, and facilitating automated scanning of the fluorescent FISH specimens for simplified enumeration on various backend scanning and analysis systems. The results indicated that ALK FISH can be automated. Significantly, both the Ikoniscope and BioView system of automated FISH scanning and analysis systems provided a robust analysis algorithm to define ALK rearrangements. In addition, the BioView system facilitated consultation of difficult cases via the internet.

  18. Detection of a complex translocation using fluorescent in situ hybridization (FISH)

    Energy Technology Data Exchange (ETDEWEB)

    Rosen, B.A. [Brandeis Univ., Waltham, MA (United States); Abuelo, D.N. [Rhode Island Hospital, Providence, RI (United States); Mark, H.F. [Brown Univ. School of Medicine, Providence, RI (United States)

    1994-09-01

    The use of fluorescent in situ hybridization (FISH) allowed the detection of a complex 3-way translocation in a patient with multiple congenital malformations and mental retardation. The patient was a 10-year-old girl with mental retardation, seizures, repaired cleft palate, esotropia, epicanthal folds, broad nasal bridge, upward slanting palpebral fissures, single transverse palmar crease, brachydactyly, hypoplastic nails, ectrodactyly between the third and fourth right toes, and hypoplasia of the left third toe. Chromosome analysis performed at birth was reported as normal. We performed high resolution banding analysis which revealed an apparently balanced translocation between chromosomes 2 and 9. However, because of her multiple abnormalities, further studies were ordered. Fluorescent in situ hybridization (FISH) using chromosome painting probes revealed a karyotype of 46,XX,t(2;8;9) (2pter{yields}q31::8q21.2{yields}8qter; 8pter{yields}q21.2::2q31{yields}q34::9q34{yields}qter; 9pter{yields}q34::2q34{yields}qter). The 3-way translocation appears to be de novo, as neither parent is a translocation carrier. This case illustrates the importance of using FISH to further investigate cases of apparently balanced translocations in the presence of phenotypic abnormalities and/or mental retardation.

  19. Detection of Helicobacter pylori in raw bovine milk by fluorescence in situ hybridization (FISH).

    Science.gov (United States)

    Angelidis, Apostolos S; Tirodimos, Ilias; Bobos, Mattheos; Kalamaki, Mary S; Papageorgiou, Demetrios K; Arvanitidou, Malamatenia

    2011-12-02

    The transmission pathways of Helicobacter pylori in humans have not been fully elucidated. Research in the last decade has proposed that foodborne transmission, among others, may be a plausible route of human infection. Owing to the organism's fastidious growth characteristics and its ability to convert to viable, yet unculturable states upon exposure to stress conditions, the detection of H. pylori in foods via culture-dependent methods has been proven to be laborious, difficult and in most cases unsuccessful. Hence, nucleic acid-based methods have been proposed as alternative methods but, to date, only PCR-based methods have been reported in the literature. In the current study, fluorescence in situ hybridization (FISH) was used for the detection of H. pylori in raw, bulk-tank bovine milk. After repeated milk centrifugation and washing steps, the bacterial flora of raw milk was subjected to fixation and permeabilization and H. pylori detection was conducted by FISH after hybridization with an H. pylori-specific 16S rRNA-directed fluorescent oligonucleotide probe. Using this protocol, H. pylori was detected in four out of the twenty (20%) raw milk samples examined. The data presented in this manuscript indicate that FISH can serve as an alternative molecular method for screening raw bovine milk for the presence of H. pylori.

  20. Segregation of Species-Specific Male Attractiveness in F2 Hybrid Lake Malawi Cichlid Fish

    Directory of Open Access Journals (Sweden)

    Ola Svensson

    2011-01-01

    Full Text Available Among the huge radiations of haplochromine cichlid fish in Lakes Malawi and Victoria, closely related species are often reproductively isolated via female mate choice although viable fertile hybrids can be produced when females are confined only with heterospecific males. We generated F2 hybrid males from a cross between a pair of closely related sympatric cichlid fish from Lake Malawi. Laboratory mate choice experiments using microsatellite paternity analysis demonstrated that F2 hybrid males differed significantly in their attractiveness to females of the two parental species, indicating heritable variation in traits involved in mate choice that may contribute to reproductive isolation between these species. We found no significant correlation between male mating success and any measurement of male colour pattern. A simple quantitative genetic model of reproductive isolation suggests that there may be as few as two chromosomal regions controlling species-specific attractiveness. We propose that adaptive radiation of Lake Malawi cichlids could be facilitated by the presence of genes with major effects on mate choice and reproductive isolation.

  1. Water quality and plankton communities in hybrid catfish (female channel catfish, Ictalurus punctatus x male blue catfish, I. furcatus) ponds after partial fish harvest

    Science.gov (United States)

    Twelve, 0.4-ha ponds were stocked with 10,000 hybrid catfish fingerlings in March 2015. Six ponds were partially harvested in August to remove fish larger than ~ 0.57 kg. All remaining fish were removed in October and November. Partial harvest of faster-growing fish removed ~26% of fish initially st...

  2. The FISH chromosome painting technique in Biological Dosimetry : Evolution and applicability; La Tecnica de Pintado Cromosomico mediante FISH en Dosimetria Biologica: Evolucion y Aplicabilidad

    Energy Technology Data Exchange (ETDEWEB)

    Oteo, M.; Real, A.

    2005-07-01

    The capacity of ionising radiation to induce chromosomal aberrations has been used during decades to assess doses in persons accidentally exposed, giving rise to the discipline Biological Dosimetry. the Biological Dosimetry is a complement to the physical methods of dosimetry and in the situations where those are not available represent the only way to asses the doses received. During decades biological dosimetry has used the dicentric score to determine doses, since a good correlation between in vitro and in vivo irradiation results exists and the background yields are low. However, dicentrics yields decrease with time after irradiation. The translocations and insertions represent stable aberrations what means that they do not decrease in further cell divisions. The development at the end of the 80s of the FISH technique (Fluorescence In Situ Hybridization), represented a great step for the applicability of translocations score for biological dosimetry. In this paper, a review of the FISH chromosome painting technique is presented, addressing the facts that have needed to be solve before its routine use in retrospective dosimetry, as well as the aspects that will need further research in the near future. (Author) 54 refs.

  3. Validation of interphase fluorescence in situ hybridization (iFISH for multiple myeloma using CD138 positive cells

    Directory of Open Access Journals (Sweden)

    Renata Kiyomi Kishimoto

    2016-06-01

    Full Text Available ABSTRACT BACKGROUND: Multiple myeloma is a plasma cell neoplasm with acquired genetic abnormalities of clinical and prognostic importance. Multiple myeloma differs from other hematologic malignancies due to a high fraction of low proliferating malignant plasma cells and the paucity of plasma cells in bone marrow aspiration samples, making cytogenetic analysis a challenge. An abnormal karyotype is found in only one-third of patients with multiple myeloma and interphase fluorescence in situ hybridization is the most useful test for studying the chromosomal abnormalities present in almost 90% of cases. However, it is necessary to study the genetic abnormalities in plasma cells after their identification or selection by morphology, immunophenotyping or sorting. Other challenges are the selection of the most informative FISH panel and determining cut-off levels for FISH probes. This study reports the validation of interphase fluorescence in situ hybridization using CD138 positive cells, according to proposed guidelines published by the European Myeloma Network (EMN in 2012. METHOD: Bone marrow samples from patients with multiple myeloma were used to standardize a panel of five probes [1q amplification, 13q14 deletion, 17p deletion, t(4;14, and t(14;16] in CD138+ cells purified by magnetic cell sorting. RESULTS: This test was validated with a low turnaround time and good reproducibility. Five of six samples showed genetic abnormalities. Monosomy/deletion 13 plus t(4;14 were found in two cases. CONCLUSION: This technique together with magnetic cell sorting is effective and can be used in the routine laboratory practice. In addition, magnetic cell sorting provides a pure plasma cell population that allows other molecular and genomic studies.

  4. Insertional translocation detected using FISH confirmation of array-comparative genomic hybridization (aCGH) results.

    Science.gov (United States)

    Kang, Sung-Hae L; Shaw, Chad; Ou, Zhishuo; Eng, Patricia A; Cooper, M Lance; Pursley, Amber N; Sahoo, Trilochan; Bacino, Carlos A; Chinault, A Craig; Stankiewicz, Pawel; Patel, Ankita; Lupski, James R; Cheung, Sau Wai

    2010-05-01

    Insertional translocations (ITs) are rare events that require at least three breaks in the chromosomes involved and thus qualify as complex chromosomal rearrangements (CCR). In the current study, we identified 40 ITs from approximately 18,000 clinical cases (1:500) using array-comparative genomic hybridization (aCGH) in conjunction with fluorescence in situ hybridization (FISH) confirmation of the aCGH findings, and parental follow-up studies. Both submicroscopic and microscopically visible IT events were detected. They were divided into three major categories: (1) simple intrachromosomal and interchromosomal IT resulting in pure segmental trisomy, (2) complex IT involving more than one abnormality, (3) deletion inherited from a parent with a balanced IT resulting in pure segmental monosomy. Of the cases in which follow-up parental studies were available, over half showed inheritance from an apparently unaffected parent carrying the same unbalanced rearrangement detected in the propositi, thus decreasing the likelihood that these IT events are clinically relevant. Nevertheless, we identified six cases in which small submicroscopic events were detected involving known disease-associated genes/genomic segments and are likely to be pathogenic. We recommend that copy number gains detected by clinical aCGH analysis should be confirmed using FISH analysis whenever possible in order to determine the physical location of the duplicated segment. We hypothesize that the increased use of aCGH in the clinic will demonstrate that IT occurs more frequently than previously considered but can identify genomic rearrangements with unclear clinical significance.

  5. Fluorescence in situ hybridization (FISH analysis of primary ocular adnexal MALT lymphoma

    Directory of Open Access Journals (Sweden)

    Harada Mine

    2006-10-01

    Full Text Available Abstract Background It remains unknown whether primary ocular adnexal extranodal marginal zone B-cell lymphoma of mucosa-associated lymphoid tissue (MALT lymphoma is a homogeneous entity, as there are few reports of the results of cytogenetic or molecular analyses of these tumors. Methods We performed interphase fluorescence in situ hybridization (FISH analysis to detect translocations and aneuploidy in 34 cases of primary ocular adnexal MALT lymphoma, and reviewed the histopathological findings. Correlations between the results of FISH analysis, the histopathological features and the clinical data were also analyzed. Results Among the 34 cases, FISH analysis revealed t(14;18(q32;q21 in one case, trisomy 3 in 21 cases (62%, and trisomy 18 in 16 cases (47%. The cases with trisomy 18 had significantly more prominent lymphoepithelial lesions (LELs and less nodularity in the tumors. In regard to the clinical correlations, tumors with trisomy 18 were observed predominantly in females and younger patients; also, in the majority of the cases, the tumor was of conjunctival origin. All the cases with recurrence showed trisomy 18 in the tumor. Conclusion Primary ocular adnexal MALT lymphoma is a significantly heterogeneous entity. Cases with trisomy 18 may have unique clinicopathological features.

  6. OFF-LINE HANDWRITING RECOGNITION USING VARIOUS HYBRID MODELING TECHNIQUES AND CHARACTER N-GRAMS

    NARCIS (Netherlands)

    Brakensiek, A.; Rottland, J.; Kosmala, A.; Rigoll, G.

    2004-01-01

    In this paper a system for on-line cursive handwriting recognition is described. The system is based on Hidden Markov Models (HMMs) using discrete and hybrid modeling techniques. Here, we focus on two aspects of the recognition system. First, we present different hybrid modeling techniques, whereas

  7. Metabolic responses of fish following exposure to two different oil spill remediation techniques.

    Science.gov (United States)

    Cohen, A; Nugegoda, D; Gagnon, M M

    2001-03-01

    To assess the impacts of two oil spill remediation techniques on fish metabolism, change in aerobic and anaerobic enzyme activities in juvenile Australian Bass, Macquaria novemaculeata, was examined. Changes in cytochrome C oxidase (CCO) and lactate dehydrogenase (LDH) activities were investigated following exposure to the crude oil water accommodated fraction (WAF) and chemically dispersed crude oil WAF. There was a significant stimulation in CCO activity in the gills and livers of fish exposed to the WAF of Bass Strait crude oil and chemically dispersed crude oil, compared to the control treatment. In addition, LDH activity was significantly stimulated in the liver of fish exposed to dispersed crude oil WAF, compared to the crude oil WAF. Fish exposed to the dispersed crude oil WAF treatment had significantly higher oxygen consumption, as measured by oxygen depletion in a sealed chamber, than fish exposed to the crude oil WAF and control treatments.

  8. Comparison of high resolution chromosome banding and fluorescence in situ hybridization (FISH) for the laboratory evaluation of Prader-Willi syndrome and Angelman syndrome

    Energy Technology Data Exchange (ETDEWEB)

    Delach, J.A.; Rosengren, S.S.; Kaplan, L.; Greenstein, R.M.; Cassidy, S.B.; Benn, P.A.

    1994-08-01

    The development of probes containing segments of DNA from chromosome region 15q11-q13 provides the opportunity to confirm the diagnosis of Prader-Willi syndrome (PWS) and Angelman syndrome (AS) by fluorescence in situ hybridization (FISH). We have evaluated FISH studies and high resolution chromosome banding studies in 14 patients referred to confirm or rule out AS. In four patients (three from the PWS category and 1 from the AS group) chromosome analysis suggested that a deletion was present but FISH failed to confirm the finding. In one AS group patient, FISH identified a deletion not detectable by high resolution banding. Review of the clinical findings in the discrepant cases suggested that FISH results were correct and high resolution findings were erroneous. Studies with a chromosome 15 alpha satellite probe (D15Z) on both normal and abnormal individuals suggested that incorrect interpretation of chromosome banding may occasionally be attributable to alpha satellite polymorphism but other variation of 15q11-q13 chromosome bands also contributes to misinterpretation. We conclude that patients who have been reported to have a cytogenetic deletion of 15q11-q13 and who have clinical findings inconsistent with PWS and AS should be re-evaluated by molecular genetic techniques. 31 refs., 3 figs., 2 tabs.

  9. Trade-off between morphological convergence and opportunistic diet behavior in fish hybrid zone

    Directory of Open Access Journals (Sweden)

    Grey Jonathan

    2009-10-01

    Full Text Available Abstract Background The invasive Chondrostoma nasus nasus has colonized part of the distribution area of the protected endemic species Chondrostoma toxostoma toxostoma. This hybrid zone is a complex system where multiple effects such as inter-species competition, bi-directional introgression, strong environmental pressure and so on are combined. Why do sympatric Chondrostoma fish present a unidirectional change in body shape? Is this the result of inter-species interactions and/or a response to environmental effects or the result of trade-offs? Studies focusing on the understanding of a trade-off between multiple parameters are still rare. Although this has previously been done for Cichlid species flock and for Darwin finches, where mouth or beak morphology were coupled to diet and genetic identification, no similar studies have been done for a fish hybrid zone in a river. We tested the correlation between morphology (body and mouth morphology, diet (stable carbon and nitrogen isotopes and genomic combinations in different allopatric and sympatric populations for a global data set of 1330 specimens. To separate the species interaction effect from the environmental effect in sympatry, we distinguished two data sets: the first one was obtained from a highly regulated part of the river and the second was obtained from specimens coming from the less regulated part. Results The distribution of the hybrid combinations was different in the two part of the sympatric zone, whereas all the specimens presented similar overall changes in body shape and in mouth morphology. Sympatric specimens were also characterized by a larger diet behavior variance than reference populations, characteristic of an opportunistic diet. No correlation was established between the body shape (or mouth deformation and the stable isotope signature. Conclusion The Durance River is an untamed Mediterranean river despite the presence of numerous dams that split the river from

  10. Trade-off between morphological convergence and opportunistic diet behavior in fish hybrid zone.

    Science.gov (United States)

    Corse, Emmanuel; Costedoat, Caroline; Pech, Nicolas; Chappaz, Rémi; Grey, Jonathan; Gilles, André

    2009-10-27

    The invasive Chondrostoma nasus nasus has colonized part of the distribution area of the protected endemic species Chondrostoma toxostoma toxostoma. This hybrid zone is a complex system where multiple effects such as inter-species competition, bi-directional introgression, strong environmental pressure and so on are combined. Why do sympatric Chondrostoma fish present a unidirectional change in body shape? Is this the result of inter-species interactions and/or a response to environmental effects or the result of trade-offs? Studies focusing on the understanding of a trade-off between multiple parameters are still rare. Although this has previously been done for Cichlid species flock and for Darwin finches, where mouth or beak morphology were coupled to diet and genetic identification, no similar studies have been done for a fish hybrid zone in a river. We tested the correlation between morphology (body and mouth morphology), diet (stable carbon and nitrogen isotopes) and genomic combinations in different allopatric and sympatric populations for a global data set of 1330 specimens. To separate the species interaction effect from the environmental effect in sympatry, we distinguished two data sets: the first one was obtained from a highly regulated part of the river and the second was obtained from specimens coming from the less regulated part. The distribution of the hybrid combinations was different in the two part of the sympatric zone, whereas all the specimens presented similar overall changes in body shape and in mouth morphology. Sympatric specimens were also characterized by a larger diet behavior variance than reference populations, characteristic of an opportunistic diet. No correlation was established between the body shape (or mouth deformation) and the stable isotope signature. The Durance River is an untamed Mediterranean river despite the presence of numerous dams that split the river from upstream to downstream. The sympatric effect on morphology and

  11. 5-bp Classical Satellite DNA Loci from Chromosome-1 Instability in Cervical Neoplasia Detected by DNA Breakage Detection/Fluorescence in Situ Hybridization (DBD-FISH).

    Science.gov (United States)

    Cortés-Gutiérrez, Elva I; Ortíz-Hernández, Brenda L; Dávila-Rodríguez, Martha I; Cerda-Flores, Ricardo M; Fernández, José Luis; López-Fernández, Carmen; Gosálvez, Jaime

    2013-02-19

    We aimed to evaluate the association between the progressive stages of cervical neoplasia and DNA damage in 5-bp classical satellite DNA sequences from chromosome-1 in cervical epithelium and in peripheral blood lymphocytes using DNA breakage detection/fluorescence in situ hybridization (DBD-FISH). A hospital-based unmatched case-control study was conducted in 2011 with a sample of 30 women grouped according to disease stage and selected according to histological diagnosis; 10 with low-grade squamous intraepithelial lesions (LG-SIL), 10 with high-grade SIL (HG-SIL), and 10 with no cervical lesions, from the Unidad Medica de Alta Especialidad of The Mexican Social Security Institute, IMSS, Mexico. Specific chromosome damage levels in 5-bp classical satellite DNA sequences from chromosome-1 were evaluated in cervical epithelium and peripheral blood lymphocytes using the DBD-FISH technique. Whole-genome DNA hybridization was used as a reference for the level of damage. Results of Kruskal-Wallis test showed a significant increase according to neoplastic development in both tissues. The instability of 5-bp classical satellite DNA sequences from chromosome-1 was evidenced using chromosome-orientation FISH. In conclusion, we suggest that the progression to malignant transformation involves an increase in the instability of 5-bp classical satellite DNA sequences from chromosome-1.

  12. 5-bp Classical Satellite DNA Loci from Chromosome-1 Instability in Cervical Neoplasia Detected by DNA Breakage Detection/Fluorescence in Situ Hybridization (DBD-FISH)

    Science.gov (United States)

    Cortés-Gutiérrez, Elva I.; Ortíz-Hernández, Brenda L.; Dávila-Rodríguez, Martha I.; Cerda-Flores, Ricardo M; Fernández, José Luis; López-Fernández, Carmen; Gosálvez, Jaime

    2013-01-01

    We aimed to evaluate the association between the progressive stages of cervical neoplasia and DNA damage in 5-bp classical satellite DNA sequences from chromosome-1 in cervical epithelium and in peripheral blood lymphocytes using DNA breakage detection/fluorescence in situ hybridization (DBD-FISH). A hospital-based unmatched case-control study was conducted in 2011 with a sample of 30 women grouped according to disease stage and selected according to histological diagnosis; 10 with low-grade squamous intraepithelial lesions (LG-SIL), 10 with high-grade SIL (HG-SIL), and 10 with no cervical lesions, from the Unidad Medica de Alta Especialidad of The Mexican Social Security Institute, IMSS, Mexico. Specific chromosome damage levels in 5-bp classical satellite DNA sequences from chromosome-1 were evaluated in cervical epithelium and peripheral blood lymphocytes using the DBD-FISH technique. Whole-genome DNA hybridization was used as a reference for the level of damage. Results of Kruskal-Wallis test showed a significant increase according to neoplastic development in both tissues. The instability of 5-bp classical satellite DNA sequences from chromosome-1 was evidenced using chromosome-orientation FISH. In conclusion, we suggest that the progression to malignant transformation involves an increase in the instability of 5-bp classical satellite DNA sequences from chromosome-1. PMID:23429197

  13. 5-bp Classical Satellite DNA Loci from Chromosome-1 Instability in Cervical Neoplasia Detected by DNA Breakage Detection/Fluorescence in Situ Hybridization (DBD-FISH

    Directory of Open Access Journals (Sweden)

    Jaime Gosálvez

    2013-02-01

    Full Text Available We aimed to evaluate the association between the progressive stages of cervical neoplasia and DNA damage in 5-bp classical satellite DNA sequences from chromosome-1 in cervical epithelium and in peripheral blood lymphocytes using DNA breakage detection/fluorescence in situ hybridization (DBD-FISH. A hospital-based unmatched case-control study was conducted in 2011 with a sample of 30 women grouped according to disease stage and selected according to histological diagnosis; 10 with low-grade squamous intraepithelial lesions (LG-SIL, 10 with high-grade SIL (HG-SIL, and 10 with no cervical lesions, from the Unidad Medica de Alta Especialidad of The Mexican Social Security Institute, IMSS, Mexico. Specific chromosome damage levels in 5-bp classical satellite DNA sequences from chromosome-1 were evaluated in cervical epithelium and peripheral blood lymphocytes using the DBD-FISH technique. Whole-genome DNA hybridization was used as a reference for the level of damage. Results of Kruskal-Wallis test showed a significant increase according to neoplastic development in both tissues. The instability of 5-bp classical satellite DNA sequences from chromosome-1 was evidenced using chromosome-orientation FISH. In conclusion, we suggest that the progression to malignant transformation involves an increase in the instability of 5-bp classical satellite DNA sequences from chromosome-1.

  14. Nitrifying bacterial communities in an aquaculture wastewater treatment system using fluorescence in situ hybridization (FISH), 16S rRNA gene cloning, and phylogenetic analysis.

    Science.gov (United States)

    Paungfoo, Chanyarat; Prasertsan, Poonsuk; Burrell, Paul C; Intrasungkha, Nugul; Blackall, Linda L

    2007-07-01

    Aquaculture, especially shrimp farming, has played a major role in the growth of Thailand's economy in recent years, as well as in many South East Asian countries. However, the nutrient discharges from these activities have caused adverse impacts on the quality of the receiving waterways. In particular nitrogenous compounds, which may accumulate in aquaculture ponds, can be toxic to aquatic animals and cause environmental problems such as eutrophication. The mineralization process is well known, but certain aspects of the microbial ecology of nitrifiers, the microorganisms that convert ammonia to nitrate, are poorly understood. A previously reported enrichment of nitrifying bacteria (ammonia-oxidizing bacteria (AOB) and nitrite-oxidizing bacteria (NOB)) from a shrimp farm inoculated in a sequencing batch reactor (SBR) was studied by molecular methods. The initial identification and partial quantification of the nitrifying bacteria (AOB and NOB) were carried out by fluorescence in situ hybridization (FISH) using previously published 16S rRNA-targeting oligonucleotide probes. The two dominant bacterial groups detected by FISH were from the Cytophaga-Flavobacterium-Bacteroides and Proteobacteria (beta subdivision) phyla. Published FISH probes for Nitrobacter and Nitrospira did not hybridize to any of the bacterial cells. Therefore it is likely that new communities of NOBs, differing from previously reported ones, exist in the enrichments. Molecular genetic techniques (cloning, sequencing, and phylogenetic analysis) targeting the 16S rRNA genes from the nitrifying enrichments were performed to identify putative AOBs and NOBs.

  15. Coupling Turbulence in Hybrid LES-RANS Techniques

    Science.gov (United States)

    Woodruff, Stephen L.

    2011-01-01

    A formulation is proposed for hybrid LES-RANS computations that permits accurate computations during resolution changes, so that resolution may be changed at will in order to employ only as much resolution in each subdomain as is required by the physics. The two components of this formulation, establishing the accuracy of a hybrid model at constant resolutions throughout the RANS-to-LES range and maintaining that accuracy when resolution is varied, are demonstrated for decaying, homogeneous, isotropic turbulence.

  16. Salmonellae in fish feces analyzed by in situ hybridization and quantitative polymerase chain reaction.

    Science.gov (United States)

    Sha, Qiong; Forstner, Michael R J; Bonner, Timothy H; Hahn, Dittmar

    2013-09-01

    The potential of fish to transfer salmonellae from heterogeneous aquatic biofilms into feces was assessed in controlled aquarium studies with Suckermouth Catfish Hypostomus plecostomus and with biofilms inoculated with salmonellae. Neither the presence of catfish nor inoculation with salmonellae had detectable effects on the abundance of the microbial community. Densities of the microbial community were about 10(5) cells/mL in the water during a 1-week period, whereas densities of the microbial community increased 10-fold (10(6) to 10(7) cells/mg) in catfish feces during the same period. Salmonellae were detected by both quantitative polymerase chain reaction (qPCR) and situ hybridization in water samples immediately after inoculation, in numbers of about 10(4) cells/mL, representing up to 20% of the cells of the microbial community. Numbers decreased by three orders of magnitude within the first 3 d of the study, which represented only 0.01% of the community, and became undetectable after day 5. In catfish feces, numbers of Salmonella initially increased to up to 6% of the cells of the community but then declined. These results suggest that Salmonella are not biomagnified during gut passage, and thus, fish only provide a means for the translocation of this pathogen.

  17. Examining predictors of chemical toxicity in freshwater fish using the random forest technique.

    Science.gov (United States)

    Tuulaikhuu, Baigal-Amar; Guasch, Helena; García-Berthou, Emili

    2017-04-01

    Chemical pollution is one of the main issues globally threatening the enormous biodiversity of freshwater ecosystems. The toxicity of substances depends on many factors such as the chemical itself, the species affected, environmental conditions, exposure duration, and concentration. We used the random forest technique to examine the factors that mediate toxicity in a set of widespread fishes and analyses of covariance to further assess the importance of differential sensitivity among fish species. Among 13 variables, the 5 most important predictors of toxicity with random forests were, by order of importance, the chemical substance itself (i.e., Chemical Abstracts Service number considered as a categorical factor), octanol-water partition coefficient (log P), pollutant prioritization, ecological structure-activity relationship (ECOSAR) classification, and fish species for 50% lethal concentrations (LC50) and the chemical substance, fish species, log P, ECOSAR classification, and water temperature for no observed effect concentrations (NOECs). Fish species was a very important predictor for both endpoints and with the two contrasting statistical techniques used. Different fish species displayed very different relationships with log P, often with different slopes and with as much importance as the partition coefficient. Therefore, caution should be exercised when extrapolating toxicological results or relationships among species. In addition, further research is needed to determine species-specific sensitivities and unravel the mechanisms behind them.

  18. Asexual Reproduction Does Not Apparently Increase the Rate of Chromosomal Evolution: Karyotype Stability in Diploid and Triploid Clonal Hybrid Fish (Cobitis, Cypriniformes, Teleostei).

    Science.gov (United States)

    Majtánová, Zuzana; Choleva, Lukáš; Symonová, Radka; Ráb, Petr; Kotusz, Jan; Pekárik, Ladislav; Janko, Karel

    2016-01-01

    Interspecific hybridization, polyploidization and transitions from sexuality to asexuality considerably affect organismal genomes. Especially the last mentioned process has been assumed to play a significant role in the initiation of chromosomal rearrangements, causing increased rates of karyotype evolution. We used cytogenetic analysis and molecular dating of cladogenetic events to compare the rate of changes of chromosome morphology and karyotype in asexually and sexually reproducing counterparts in European spined loach fish (Cobitis). We studied metaphases of three sexually reproducing species and their diploid and polyploid hybrid clones of different age of origin. The material includes artificial F1 hybrid strains, representatives of lineage originated in Holocene epoch, and also individuals of an oldest known age to date (roughly 0.37 MYA). Thereafter we applied GISH technique as a marker to differentiate parental chromosomal sets in hybrids. Although the sexual species accumulated remarkable chromosomal rearrangements after their speciation, we observed no differences in chromosome numbers and/or morphology among karyotypes of asexual hybrids. These hybrids possess chromosome sets originating from respective parental species with no cytogenetically detectable recombinations, suggesting their integrity even in a long term. The switch to asexual reproduction thus did not provoke any significant acceleration of the rate of chromosomal evolution in Cobitis. Asexual animals described in other case studies reproduce ameiotically, while Cobitis hybrids described here produce eggs likely through modified meiosis. Therefore, our findings indicate that the effect of asexuality on the rate of chromosomal change may be context-dependent rather than universal and related to particular type of asexual reproduction.

  19. Identification of intracellular bacteria in adenoid and tonsil tissue specimens: the efficiency of culture versus fluorescent in situ hybridization (FISH).

    Science.gov (United States)

    Stępińska, M; Olszewska-Sosińska, O; Lau-Dworak, M; Zielnik-Jurkiewicz, B; Trafny, E A

    2014-01-01

    Monocyte/macrophage cells from human nasopharyngeal lymphoid tissue can be a source of bacteria responsible for human chronic and recurrent upper respiratory tract infection. Detection and characterization of pathogens surviving intracellularly could be a key element in bacteriological diagnosis of the infections as well as in the study on interactions between bacteria and their host. The present study was undertaken to assess the possibility of isolation of viable bacteria from the cells expressing monocyte/macrophage marker CD14 in nasopharyngeal lymphoid tissue. Overall, 74 adenotonsillectomy specimens (adenoids and tonsils) from 37 children with adenoid hypertrophy and recurrent infections as well as 15 specimens from nine children with adenoid hypertrophy, which do not suffer from upper respiratory tract infections (the control group), were studied. The suitability of immunomagnetic separation for extraction of CD14(+) cells from lymphoid tissue and for further isolation of the intracellular pathogens has been shown. The coexistence of living pathogens including Haemophilus influenzae, Staphylococcus aureus, and Streptococcus pyogenes with the bacteria representing normal nasopharyngeal microbiota inside CD14(+) cells was demonstrated. Twenty-four strains of these pathogens from 32.4 % of the lysates of CD14(+) cells were isolated. Concurrently, the fluorescent in situ hybridization (FISH) with a universal EUB388, and the species-specific probes demonstrated twice more often the persistence of these bacterial species in the lysates of CD14(+) cells than conventional culture. Although the FISH technique appears to be more sensitive than traditional culture in the intracellular bacteria identification, the doubts on whether the bacteria are alive, and therefore, pathogenic would still exist without the strain cultivation.

  20. Effects of fishing technique on assessing species composition in aquatic systems in semi-arid Brazil

    Directory of Open Access Journals (Sweden)

    ESF Medeiros

    Full Text Available In most ecological field research, appropriate sampling is critical for the understanding of processes underlying fish populations and communities, and is even more important in heterogeneous environments such as the aquatic systems of the semi-arid region of Brazil. This study intends to make a contribution to the development of sampling programs and gear selection in aquatic systems of semi-arid Brazil by evaluating the effects of different fishing techniques on the assessment of richness and composition of the fish fauna in selected aquatic environments. Six sites were selected to represent typical artificial (reservoirs and natural (intermittent streams environments and four different types of sampling gear were applied to each site during four occasions. The present study shows that when selecting sampling techniques to be used in aquatic systems in semi-arid Brazil, one must consider the objectives of the study, e.g. ecological or taxonomic, in order to decide on inclusion of rare species in the sampling population. Also, the effect of the sampling gear on natural abundances of fish must be considered given that some sampling techniques are highly detrimental to fish population numbers.

  1. Hybrid estimation technique for predicting butene concentration in polyethylene reactor

    Science.gov (United States)

    Mohd Ali, Jarinah; Hussain, M. A.

    2016-03-01

    A component of artificial intelligence (AI), which is fuzzy logic, is combined with the so-called conventional sliding mode observer (SMO) to establish a hybrid type estimator to predict the butene concentration in the polyethylene production reactor. Butene or co-monomer concentration is another significant parameter in the polymerization process since it will affect the molecular weight distribution of the polymer produced. The hybrid estimator offers straightforward formulation of SMO and its combination with the fuzzy logic rules. The error resulted from the SMO estimation will be manipulated using the fuzzy rules to enhance the performance, thus improved on the convergence rate. This hybrid estimation is able to estimate the butene concentration satisfactorily despite the present of noise in the process.

  2. Hybrid computer techniques for solving partial differential equations

    Science.gov (United States)

    Hammond, J. L., Jr.; Odowd, W. M.

    1971-01-01

    Techniques overcome equipment limitations that restrict other computer techniques in solving trivial cases. The use of curve fitting by quadratic interpolation greatly reduces required digital storage space.

  3. Karyotype of Zea luxurians and Z. mays subsp. mays using FISH/DAPI, and analysis of meiotic behavior of hybrids.

    Science.gov (United States)

    González, Graciela E; Poggio, Lidia

    2011-01-01

    The karyotypes of Zea luxurians and a race of maize from northwestern Argentina are described and compared using 4′,6-diamidino-2-phenylindole (DAPI) banding and fluorescent in situ hybridization (FISH) to localize the 180 bp knobs. The meiotic behavior of the F₁ artificial hybrids Z. luxurians × maize is also analyzed to determine the genomic relationships between both species. Neocentromere activity at knobs in the meiosis of the hybrids is particularly discussed. The meiotic behavior and the high pollen sterility of the hybrid revealed genetical and (or) chromosomal divergences, leading to postzygotic reproductive isolation among their parents. Here, we propose that maize shows lower genomic affinity to Z. luxurians than to other species of the genus with 2n = 20.

  4. 荧光原位杂交技术在血尿患者膀胱尿路上皮癌筛查中的应用%Detection of bladder cancer from the urine using fluorescence in situ hybridization(FISH) technique

    Institute of Scientific and Technical Information of China (English)

    江长琴; 梁朝朝; 叶元平; 刘明; 张贤生; 郝宗耀; 周骏; 樊松

    2010-01-01

    目的 评价荧光原位杂交(fluorescence in situ hybridization,FISH)技术在血尿患者膀胱尿路上皮癌筛查中的临床应用价值,为从尿液脱落细胞中早期诊断膀胱癌提供新的途径.方法 选择20例健康正常人,通过FISH技术检测9号染色体p16位点、3号染色体、7号染色体及17号染色体变异情况,建立阈值.100例血尿患者留取晨尿,分别进行尿脱落细胞学检查和FISH技术检查.统计分析FISH方法的敏感度和特异度.结果 探针检测p16基因完全缺失阈值为3.9%,部分缺失为5.4%,扩增为3.2%;3号染色体缺失阈值为4.0%.扩增为3.5%;7号染色体缺失阈值为4.0%,扩增为2.8%;17号染色体缺失阈值为5.8%,扩增为3.4%.以至少两种探针检测结果超过阈值或一种探针检测结果至少存在两种异常为诊断阳性.FISH技术在血尿患者膀胱尿路上皮癌筛查中的敏感度和特异度分别为93.6%和100%,尿脱落细胞学检测分别为46.8%和100%.结论 FISH技术无创快速,对血尿患者膀胱尿路上皮癌筛查的敏感度优于尿脱落细胞学检查方法.

  5. Use of the DBD-FISH technique for detecting DNA breakage in response to high doses of X-rays.

    Science.gov (United States)

    Cortés-Gutiérrez, Elva I; Dávila-Rodríguez, Martha I; Cerda-Flores, Ricardo M; Fernández, José Luis; López-Fernández, Carmen; Gosálvez, Jaime

    2014-11-01

    The aim of this study was to generate a dose-response curve using the DNA breakage detection-fluorescent in situ hybridization (DBD-FISH) test as a biomarker of initial genetic effects induced by high doses of X-rays. A dose-response curve was obtained by measuring the ex vivo responses to increasing doses (0-50 Gy) of X-rays in the peripheral blood lymphocytes of ten healthy donors. The overall dose-response curve was constructed using integrated density (ID; area × fluorescence intensity) as a measure of genetic damage induced by irradiation. The correlation coefficient was high (r = 0.934, b(0) = 10.408, and b(1) = 0.094). One-way ANOVA with the Student-Newman-Keuls test for multiple comparisons showed significant differences among the average ln ID values according to dose. Our results suggest the usefulness of the DBD-FISH technique for measuring intrinsic individual cellular radio sensitivity ex vivo.

  6. Multiplex fluorescence in situ hybridization (M-FISH) and confocal laser scanning microscopy (CLSM) to analyze multispecies oral biofilms.

    Science.gov (United States)

    Karygianni, Lamprini; Hellwig, Elmar; Al-Ahmad, Ali

    2014-01-01

    Multiplex fluorescence in situ hybridization (M-FISH) constitutes a favorable microbiological method for the analysis of spatial distribution of highly variable phenotypes found in multispecies oral biofilms. The combined use of confocal laser scanning microscopy (CLSM) produces high-resolution three-dimensional (3D) images of individual bacteria in their natural environment. Here, we describe the application of M-FISH on early (Streptococcus spp., Actinomyces naeslundii) and late colonizers (Fusobacterium nucleatum, Veillonella spp.) of in situ-formed oral biofilms, the acquisition of CLSM images, as well as the qualitative and quantitative analysis of these digitally obtained and processed images.

  7. A Hybrid Islanding Detection Technique Using Average Rate of Voltage Change and Real Power Shift

    DEFF Research Database (Denmark)

    Mahat, Pukar; Chen, Zhe; Bak-Jensen, Birgitte

    2009-01-01

    technique is proposed to solve this problem. An average rate of voltage change (passive technique) has been used to initiate a real power shift (active technique), which changes the eal power of distributed generation (DG), when the passive technique cannot have a clear discrimination between islanding......The mainly used islanding detection techniques may be classified as active and passive techniques. Passive techniques don't perturb the system but they have larger nondetection znes, whereas active techniques have smaller nondetection zones but they perturb the system. In this paper, a new hybrid...

  8. Direct detection of expanded trinucleotide repeats using DNA hybridization techniques

    Energy Technology Data Exchange (ETDEWEB)

    Petronis, A.; Tatuch, Y.; Kennedy, J.L. [Univ. of Toronto (Canada)] [and others

    1994-09-01

    Recently, unstable trinucleotide repeats have been shown to be the etiologic factor in several neuropsychiatric diseases, and they may play a similar role in other disorders. To our knowledge, a method that detects expanded trinucleotide sequences with the opportunity for direct localization and cloning has not been achieved. We have developed a set of hybridization-based methods for direct detection of unstable DNA expansion. Our analysis of myotonic dystrophy patients that possess different degrees of (CTG){sub n} expansion, versus unaffected controls, has demonstrated the identification of the trinucleotide instability site without any prior information regarding genetic map location. High stringency modified Southern blot hybridization with a PCR-generated trinucleotide repeat probe allowed us to detect the DNA fragment containing the expansion in myotonic dystrophy patients. The same probe was used for fluorescent in situ hybridization and several regions of (CTG){sub n}/(CAG){sub n} repeats in the human genome were detected, including the myotonic dystrophy locus on chromosome 19q. These strategies can be applied to directly clone genes involved in disorders caused by unstable DNA.

  9. Application of asymptotic waveform approximation technique to hybrid FE/BI method for 3D scattering

    Institute of Scientific and Technical Information of China (English)

    PENG Zhen; SHENG XinQing

    2007-01-01

    The asymptotic waveform evaluation (AWE) technique is a rational function approximation method in computational mathematics, which is used in many applications in computational electromagnetics. In this paper, the performance of the AWE technique in conjunction with hybrid finite element/boundary integral (FE/BI) method is firstly investigated. The formulation of the AWE applied in hybrid FE/BI method is given in detail. The characteristic implementation of the application of the AWE to the hybrid FE/BI method is discussed. Numerical results demonstrate that the AWE technique can greatly speed up the hybrid FE/BI method to acquire wide-band and wide-angle backscatter radar-cross-section (RCS) by complex targets.

  10. Does Angling Technique Selectively Target Fishes Based on Their Behavioural Type?

    Directory of Open Access Journals (Sweden)

    Alexander D M Wilson

    Full Text Available Recently, there has been growing recognition that fish harvesting practices can have important impacts on the phenotypic distributions and diversity of natural populations through a phenomenon known as fisheries-induced evolution. Here we experimentally show that two common recreational angling techniques (active crank baits versus passive soft plastics differentially target wild largemouth bass (Micropterus salmoides and rock bass (Ambloplites rupestris based on variation in their behavioural tendencies. Fish were first angled in the wild using both techniques and then brought back to the laboratory and tested for individual-level differences in common estimates of personality (refuge emergence, flight-initiation-distance, latency-to-recapture and with a net, and general activity in an in-lake experimental arena. We found that different angling techniques appear to selectively target these species based on their boldness (as characterized by refuge emergence, a standard measure of boldness in fishes but not other assays of personality. We also observed that body size was independently a significant predictor of personality in both species, though this varied between traits and species. Our results suggest a context-dependency for vulnerability to capture relative to behaviour in these fish species. Ascertaining the selective pressures angling practices exert on natural populations is an important area of fisheries research with significant implications for ecology, evolution, and resource management.

  11. Studies of the Ecophysiology of Single Cells in Microbial Communities by (Quantitative) Microautoradiography and Fluorescence In Situ Hybridization (MAR-FISH)

    DEFF Research Database (Denmark)

    Nierychlo, Marta; Nielsen, Jeppe Lund; Nielsen, Per Halkjær

    2015-01-01

    Microautoradiography (MAR) in combination with fluorescence in situ hybridization (FISH) is a powerful method of obtaining information about the ecophysiology of probe-defined single cells in mixed microbial communities. The incorporation of radiolabelled substrates can be quantified by automated...

  12. Resizing Technique-Based Hybrid Genetic Algorithm for Optimal Drift Design of Multistory Steel Frame Buildings

    Directory of Open Access Journals (Sweden)

    Hyo Seon Park

    2014-01-01

    Full Text Available Since genetic algorithm-based optimization methods are computationally expensive for practical use in the field of structural optimization, a resizing technique-based hybrid genetic algorithm for the drift design of multistory steel frame buildings is proposed to increase the convergence speed of genetic algorithms. To reduce the number of structural analyses required for the convergence, a genetic algorithm is combined with a resizing technique that is an efficient optimal technique to control the drift of buildings without the repetitive structural analysis. The resizing technique-based hybrid genetic algorithm proposed in this paper is applied to the minimum weight design of three steel frame buildings. To evaluate the performance of the algorithm, optimum weights, computational times, and generation numbers from the proposed algorithm are compared with those from a genetic algorithm. Based on the comparisons, it is concluded that the hybrid genetic algorithm shows clear improvements in convergence properties.

  13. When biogeographical provinces collide: Hybridization of reef fishes at the crossroads of marine biogeographical provinces in the Arabian Sea

    KAUST Repository

    DiBattista, Joseph

    2015-04-01

    Aim: Suture zones are areas where closely related species from different biogeographical regions come into contact and interbreed. This concept originated from the study of terrestrial ecosystems but it remains unclear whether a similar phenomenon occurs in the marine environment. Here we investigate a potential suture zone from a previously unknown hybrid hotspot at the Socotra Archipelago (Yemen), located in the Arabian Sea, where fauna from the Red Sea, Gulf of Aden, Arabian Sea, western Indian Ocean and greater Indo-Polynesian Province intersect. Location: Red Sea, Gulf of Aden, Arabian Sea and Indian Ocean. Methods: Putative hybrid reef fish were identified based on intermediate coloration and morphology. Underwater observations and collections were conducted to determine: (1) whether parent species form heterospecific social groups or breeding pairs; (2) the sex and reproductive status of morphologically intermediate individuals; and (3) whether parent species were forming mixed species associations owing to a dearth of conspecific partners. To support hybrid status, morphologically intermediate and parental individuals were genotyped using mitochondrial DNA cytochrome c oxidase subunit I (COI), nuclear recombination-activating gene 2 (RAG2) and the nuclear TMO-4C4 (TMO) gene. Results: We observed putative hybrids involving 14 species from four reef fish families at Socotra. Most cases involved a parental species with a restricted distribution (e.g. Red Sea or Arabian Sea) and a broadly distributed Indo-Pacific species. In most cases, at least one of the parent species was rare at Socotra. Hybrid gene flow was largely unidirectional, and although introgression was rare, we found evidence that some butterflyfish and surgeonfish hybrids were fertile and formed breeding groups with parental species. Main conclusions: The rate of hybrid discovery at Socotra is much greater than that recorded elsewhere in the marine environment and involved both allopatric and

  14. A Hybrid IMRT/VMAT Technique for the Treatment of Nasopharyngeal Cancer

    Directory of Open Access Journals (Sweden)

    Nan Zhao

    2015-01-01

    Full Text Available Hybrid IMRT/VMAT technique which combined intensity modulated radiotherapy (IMRT and volumetric modulated arc therapy (VMAT was developed for the treatment of nasopharyngeal cancer (NPC. Two-full-arc VMAT (2ARC-VMAT, 9-field IMRT (9F-IMRT, and Hybrid IMRT/VMAT plans for NPC were compared in terms of the dosimetric quality, sparing of organs at risk (OARs, and delivery efficiency. The Hybrid IMRT/VMAT technique can improve the target dose homogeneity and conformity compared with 9F-IMRT and 2ARC-VMAT. It can reduce the dose delivered to the TMJ, mandible, temporal lobe, and unspecified tissue with fewer MUs compared with 9F-IMRT and dose delivered to parotids, brainstem, and spinal cord compared with 2ARC-VMAT technique. The mean delivery time of Hybrid plans was shorter than that of 9F-IMRT plans (408 s versus 812 s; P=0.00 and longer than that of 2ARC-VMAT plans (408 s versus 179 s; P=0.00. Hybrid IMRT/VMAT technique could be a viable radiotherapy technique with better plan quality.

  15. A hybrid IMRT/VMAT technique for the treatment of nasopharyngeal cancer.

    Science.gov (United States)

    Zhao, Nan; Yang, Ruijie; Jiang, Yuliang; Tian, Suqing; Guo, Fuxin; Wang, Junjie

    2015-01-01

    Hybrid IMRT/VMAT technique which combined intensity modulated radiotherapy (IMRT) and volumetric modulated arc therapy (VMAT) was developed for the treatment of nasopharyngeal cancer (NPC). Two-full-arc VMAT (2ARC-VMAT), 9-field IMRT (9F-IMRT), and Hybrid IMRT/VMAT plans for NPC were compared in terms of the dosimetric quality, sparing of organs at risk (OARs), and delivery efficiency. The Hybrid IMRT/VMAT technique can improve the target dose homogeneity and conformity compared with 9F-IMRT and 2ARC-VMAT. It can reduce the dose delivered to the TMJ, mandible, temporal lobe, and unspecified tissue with fewer MUs compared with 9F-IMRT and dose delivered to parotids, brainstem, and spinal cord compared with 2ARC-VMAT technique. The mean delivery time of Hybrid plans was shorter than that of 9F-IMRT plans (408 s versus 812 s; P=0.00) and longer than that of 2ARC-VMAT plans (408 s versus 179 s; P=0.00). Hybrid IMRT/VMAT technique could be a viable radiotherapy technique with better plan quality.

  16. A Hybrid IMRT/VMAT Technique for the Treatment of Nasopharyngeal Cancer

    Science.gov (United States)

    Zhao, Nan; Yang, Ruijie; Jiang, Yuliang; Tian, Suqing; Guo, Fuxin; Wang, Junjie

    2015-01-01

    Hybrid IMRT/VMAT technique which combined intensity modulated radiotherapy (IMRT) and volumetric modulated arc therapy (VMAT) was developed for the treatment of nasopharyngeal cancer (NPC). Two-full-arc VMAT (2ARC-VMAT), 9-field IMRT (9F-IMRT), and Hybrid IMRT/VMAT plans for NPC were compared in terms of the dosimetric quality, sparing of organs at risk (OARs), and delivery efficiency. The Hybrid IMRT/VMAT technique can improve the target dose homogeneity and conformity compared with 9F-IMRT and 2ARC-VMAT. It can reduce the dose delivered to the TMJ, mandible, temporal lobe, and unspecified tissue with fewer MUs compared with 9F-IMRT and dose delivered to parotids, brainstem, and spinal cord compared with 2ARC-VMAT technique. The mean delivery time of Hybrid plans was shorter than that of 9F-IMRT plans (408 s versus 812 s; P = 0.00) and longer than that of 2ARC-VMAT plans (408 s versus 179 s; P = 0.00). Hybrid IMRT/VMAT technique could be a viable radiotherapy technique with better plan quality. PMID:25688371

  17. Test and numerical simulation of a new type of hybrid control technique

    Institute of Scientific and Technical Information of China (English)

    Meng Qingli; Zhang Minzheng; Cheng Dong

    2005-01-01

    In this paper, a new hybrid control technique, based on a combination of base-isolation and semi-active variable stiffness/damping in a superstructure, is presented. To illustrate the efficiency of the proposed control system, model tests on a mini-electromagnetic shaking table and a numerical simulation were performed. The test and numerical calculation results indicate that this new hybrid control mode with additional damping and smaller additional stiffness can achieve a better control efficiency.

  18. Variability of ribosomal DNA sites in Festuca pratensis, Lolium perenne, and their intergeneric hybrids, revealed by FISH and GISH.

    Science.gov (United States)

    Ksiazczyk, T; Taciak, M; Zwierzykowski, Z

    2010-01-01

    This study focuses on the variability of chromosomal location and number of ribosomal DNA (rDNA) sites in some diploid and autotetraploid Festuca pratensis and Lolium perenne cultivars, as well as on identification of rDNA-bearing chromosomes in their triploid and tetraploid F. pratensis × L. perenne hybrids. The rDNA loci were mapped using fluorescence in situ hybridization (FISH) with 5S and 25S rDNA probes, and the origin of parental genomes was verified by genomic in situ hybridization (GISH) with L. perenne genomic DNA as a probe, and F. pratensis genomic DNA as a block. FISH detected variation in the number and chromosomal location of both 5S and 45S rDNA sites. In F. pratensis mostly additional signals of 5S rDNA loci occurred, as compared with standard F. pratensis karyotypes. Losses of 45S rDNA loci were more frequent in L. perenne cultivars and intergeneric hybrids. Comparison of the F. pratensis and L. perenne genomes approved a higher number of rDNA sites as well as variation in chromosomal rDNA location in L. perenne. A greater instability of F. pratensis-genome-like and L. perenne-genome-like chromosomes in tetraploid hybrids was revealed, indicating gains and losses of rDNA loci, respectively. Our data indicate that the rDNA loci physically mapped on chromosomes 2 and 3 in F. pratensis and on chromosome 3 in L. perenne are useful markers for these chromosomes in intergeneric Festuca × Lolium hybrids.

  19. Management of internal resorption of central incisor using hybrid technique.

    Science.gov (United States)

    Gayathri, Prabakaran; Pandey, Ramesh Kumar; Jain, Eesha

    2014-01-28

    Internal inflammatory root resorption is characterised by progressive destruction of intraradicular dentin and dentinal tubules along the root canal wall. A number of theories have been proposed as a possible cause for internal resorption. It is usually asymptomatic and detected during routine radiographic investigations. Prompt diagnosis and early management of such defects is essential to maintain the integrity of the tooth. Non-surgical and surgical methods are the two main strategies involved in the management of internal resorption. The non-surgical method is usually preferred, but in cases of extensive resorption with external root perforation, surgical intervention has been advocated. The present case illustrates repair of perforating internal resorption by hybrid method, using mineral trioxide aggregate and gutta-percha, following surgical exposure. After a 10-month follow-up, no clinical and radiographic abnormalities were observed. Additionally, there was also marked reduction in periodontal pocket depth.

  20. 21 CFR 866.4700 - Automated fluorescence in situ hybridization (FISH) enumeration systems.

    Science.gov (United States)

    2010-04-01

    ... detection and enumeration of FISH signals in interphase nuclei of formalin-fixed, paraffin-embedded human.... This device is intended for in vitro diagnostic use with FISH assays as an aid in the...

  1. Constrained Optimization Based on Hybrid Evolutionary Algorithm and Adaptive Constraint-Handling Technique

    DEFF Research Database (Denmark)

    Wang, Yong; Cai, Zixing; Zhou, Yuren

    2009-01-01

    A novel approach to deal with numerical and engineering constrained optimization problems, which incorporates a hybrid evolutionary algorithm and an adaptive constraint-handling technique, is presented in this paper. The hybrid evolutionary algorithm simultaneously uses simplex crossover and two...... mutation operators to generate the offspring population. Additionally, the adaptive constraint-handling technique consists of three main situations. In detail, at each situation, one constraint-handling mechanism is designed based on current population state. Experiments on 13 benchmark test functions...... and four well-known constrained design problems verify the effectiveness and efficiency of the proposed method. The experimental results show that integrating the hybrid evolutionary algorithm with the adaptive constraint-handling technique is beneficial, and the proposed method achieves competitive...

  2. Large-scale multi-zone optimal power dispatch using hybrid hierarchical evolution technique

    Directory of Open Access Journals (Sweden)

    Manjaree Pandit

    2014-03-01

    Full Text Available A new hybrid technique based on hierarchical evolution is proposed for large, non-convex, multi-zone economic dispatch (MZED problems considering all practical constraints. Evolutionary/swarm intelligence-based optimisation techniques are reported to be effective only for small/medium-sized power systems. The proposed hybrid hierarchical evolution (HHE algorithm is specifically developed for solving large systems. The HHE integrates the exploration and exploitation capabilities of particle swarm optimisation and differential evolution in a novel manner such that the search efficiency is improved substantially. Most hybrid techniques export or exchange features or operations from one algorithm to the other, but in HHE their entire individual features are retained. The effectiveness of the proposed algorithm has been verified on six-test systems having different sizes and complexity levels. Non-convex MZED solution for such large and complex systems has not yet been reported.

  3. Single Molecule Techniques for Advanced in situ Hybridization

    Energy Technology Data Exchange (ETDEWEB)

    Hollars, C W; Stubbs, L; Carlson, K; Lu, X; Wehri, E

    2003-02-03

    One of the most significant achievements of modern science is completion of the human genome sequence, completed in the year 2000. Despite this monumental accomplishment, researchers have only begun to understand the relationships between this three-billion-nucleotide genetic code and the regulation and control of gene and protein expression within each of the millions of different types of highly specialized cells. Several methodologies have been developed for the analysis of gene and protein expression in situ, yet despite these advancements, the pace of such analyses is extremely limited. Because information regarding the precise timing and location of gene expression is a crucial component in the discovery of new pharmacological agents for the treatment of disease, there is an enormous incentive to develop technologies that accelerate the analytical process. Here we report on the use of plasmon resonant particles as advanced probes for in situ hybridization. These probes are used for the detection of low levels of gene-probe response and demonstrate a detection method that enables precise, simultaneous localization within a cell of the points of expression of multiple genes or proteins in a single sample.

  4. Fluorescent in-situ hybridization (FISH for BCR/ABL in chronic myeloid leukemia after bone marrow transplantation

    Directory of Open Access Journals (Sweden)

    Maria de Lourdes Lopes Ferrari Chauffaille

    2001-01-01

    Full Text Available CONTEXT: Identification of Philadelphia chromosome or BCR/ABL gene rearrangement in chronic myeloid leukemia is important at diagnosis as well as after treatment. OBJECTIVE: To compare the results of karyotyping using fluorescent in-situ hybridization (FISH upon diagnosis and 1 year after bone marrow transplantation in 12 patients. TYPE OF STUDY: Diagnostic test and residual disease detection. SETTING: Hematology and Hemotherapy Department, Federal University of São Paulo/Escola Paulista de Medicina, São Paulo, Brazil. SAMPLE: 12 patients with chronic myeloid leukemia at diagnosis and 1 year after bone marrow transplantation. DIAGNOSTIC TEST: Karyotyping was done in the usual way and the BCR/ABL gene-specific probe was used for FISH. MAIN MEASUREMENTS: Disease at diagnosis and residual. RESULTS: At diagnosis, 10 patients presented t(9;22(q34.1;q11 as well as positive FISH. Two cases did not have metaphases but FISH was positive. After bone marrow transplantation, 8 patients presented normal karyotype, 1 had persistence of identifiable Philadelphia chromosome and 3 had no metaphases. Two cases showed complete chimera and 2 had donor and host cells simultaneously. FISH was possible in all cases after bone marrow transplantation and confirmed the persistence of identifiable Philadelphia chromosome clone in one patient, and identified another that did not present metaphases for analysis. Cases that showed mixed chimera in karyotype were negative for BCR/ABL by FISH. CONCLUSION: The applicability of FISH is clear, particularly for residual disease detection. Classical and molecular cytogenetics are complementary methods.

  5. A hybrid classifier using the parallelepiped and Bayesian techniques. [for multispectral image data

    Science.gov (United States)

    Addington, J. D.

    1975-01-01

    A versatile classification scheme is developed which uses the best features of the parallelepiped algorithm and the Bayesian maximum likelihood algorithm. The parallelepiped technique has the advantage of being very fast, especially when implemented into a table look-up scheme; its disadvantage is its inability to distinguish and classify spectral signatures which are similar in nature. This disadvantage is eliminated by the Bayesian technique which is capable of distinguishing subtle differences very well. The hybrid algorithm developed reduces computer time by as much as 90%. A two- and n-dimensional description of the hybrid classifier is given.

  6. Brain tumor segmentation based on a hybrid clustering technique

    Directory of Open Access Journals (Sweden)

    Eman Abdel-Maksoud

    2015-03-01

    This paper presents an efficient image segmentation approach using K-means clustering technique integrated with Fuzzy C-means algorithm. It is followed by thresholding and level set segmentation stages to provide an accurate brain tumor detection. The proposed technique can get benefits of the K-means clustering for image segmentation in the aspects of minimal computation time. In addition, it can get advantages of the Fuzzy C-means in the aspects of accuracy. The performance of the proposed image segmentation approach was evaluated by comparing it with some state of the art segmentation algorithms in case of accuracy, processing time, and performance. The accuracy was evaluated by comparing the results with the ground truth of each processed image. The experimental results clarify the effectiveness of our proposed approach to deal with a higher number of segmentation problems via improving the segmentation quality and accuracy in minimal execution time.

  7. Determination of moisture content and water activity in algae and fish by thermoanalytical techniques

    Directory of Open Access Journals (Sweden)

    Vilma Mota da Silva

    2008-01-01

    Full Text Available The water content in seafoods is very important since it affects their sensorial quality, microbiological stability, physical characteristics and shelf life. In this study, thermoanalytical techniques were employed to develop a simple and accurate method to determine water content (moisture by thermogravimetry (TG and water activity from moisture content values and freezing point depression using differential scanning calorimetry (DSC. The precision of the results suggests that TG is a suitable technique to determine moisture content in biological samples. The average water content values for fish samples of Lutjanus synagris and Ocyurus chrysurus species were 76.4 ± 5.7% and 63.3 ± 3.9%, respectively, while that of Ulva lactuca marine algae species was 76.0 ± 4.4%. The method presented here was also successfully applied to determine water activity in two species of fish and six species of marine algae collected in the Atlantic coastal waters of Bahia, in Brazil. Water activity determined in fish samples ranged from 0.946 - 0.960 and was consistent with values reported in the literature, i.e., 0.9 - 1.0. The water activity values determined in marine algae samples lay within the interval of 0.974 - 0.979.

  8. Determination of moisture content and water activity in algae and fish by thermoanalytical techniques

    Energy Technology Data Exchange (ETDEWEB)

    Silva, Vilma Mota da; Silva, Luciana Almeida; Andrade, Jailson B. de [Universidade Federal da Bahia (UFBA), Salvador, BA (Brazil). Inst. de Quimica]. E-mail: jailsong@ufba.br; Veloso, Marcia C. da Cunha [Centro Federal de Educacao Tecnologica da Bahia (CEFET-BA), Salvador, BA (Brazil)); Santos, Gislaine Vieira [Universidade Federal da Bahia (UFBA), Salvador, BA (Brazil). Inst. de Biologia

    2008-07-01

    The water content in seafoods is very important since it affects their sensorial quality, microbiological stability, physical characteristics and shelf life. In this study, thermoanalytical techniques were employed to develop a simple and accurate method to determine water content (moisture) by thermogravimetry (TG) and water activity from moisture content values and freezing point depression using differential scanning calorimetry (DSC). The precision of the results suggests that TG is a suitable technique to determine moisture content in biological samples. The average water content values for fish samples of Lutjanus synagris and Ocyurus chrysurus species were 76.4 {+-} 5.7% and 63.3 {+-} 3.9%, respectively, while that of Ulva lactuca marine algae species was 76.0 {+-} 4.4%. The method presented here was also successfully applied to determine water activity in two species of fish and six species of marine algae collected in the Atlantic coastal waters of Bahia, in Brazil. Water activity determined in fish samples ranged from 0.946 - 0.960 and was consistent with values reported in the literature, i.e., 0.9 - 1.0. The water activity values determined in marine algae samples lay within the interval of 0.974 - 0.979. (author)

  9. Identification and characterization of marker chromosomes, de novo rearrangements and microdeletions in 100 cases with fluorescence in situ hybridization (FISH)

    Energy Technology Data Exchange (ETDEWEB)

    Anderson, S.M.; Liu, Y.; Papenhausen, P.R. [Roche Biomedical Labs., Research Triangle Park, NC (United States)

    1994-09-01

    Results of molecular cytogenetic analysis are presented for 100 cases in which fluorescence in situ hybridization (FISH) was used as an adjunct to standard cytogenetics. Commercially available centromeric, telomeric, chromosome painting and unique sequence probes were used. Cases were from a 12-month period (June 1993-May 1994) and included examples of sex chromosome abnormalities (8), duplications (5), de novo translocations (6), satellited (12) and non-satellited (7) markers, and microdeletion syndromes (62). Satellited marker chromosomes were evaluated using a combination of DAPI/Distamycin A staining, hybridization with a classical satellite probe for chromosome 15 and hybridization with alpha-satellite probes for chromosomes 13, 14, 21 and 22. Markers positive for the chromosome 15 probe were further evaluated using unique sequence probes for the Prader-Willi/Angelman region. Microdeletion analysis was performed for Prader-Willi/Angelman (49) and DiGeorge/VCF (13) syndromes. Seven cases evaluated for Prader-Willi/Angelman syndrome demonstrated evidence of a deletion within this region. Uniparental disomy analysis was available in cases where a deletion was not detected by FISH, yet follow-up was clinically indicated. Two cases evaluated for DiGeorge/VCF syndrome demonstrated molecular evidence of a deletion. Included in our analysis is an example of familial DiGeorge syndrome.

  10. Fishing

    Institute of Scientific and Technical Information of China (English)

    姜群山

    2002-01-01

    @@ Last Saturday my cousin (表兄) came to my home. We were very happy to see each other. We decided that the next day we went to fish. We got up very early that day. When we left home,the moon could still be seen in the sky.

  11. Comparison of hybrid volumetric modulated arc therapy (VMAT technique and double arc VMAT technique in the treatment of prostate cancer

    Directory of Open Access Journals (Sweden)

    Amaloo Christopher

    2015-09-01

    Full Text Available Background. Volumetric modulated arc therapy (VMAT has quickly become accepted as standard of care for the treatment of prostate cancer based on studies showing it is able to provide faster delivery with adequate target coverage and reduced monitor units while maintaining organ at risk (OAR sparing. This study aims to demonstrate the potential to increase dose conformality with increased planner control and OAR sparing using a hybrid treatment technique compared to VMAT.

  12. HybridArc: A novel radiation therapy technique combining optimized dynamic arcs and intensity modulation

    Energy Technology Data Exchange (ETDEWEB)

    Robar, James L., E-mail: james.robar@cdha.nshealth.ca [Department of Radiation Oncology, Dalhousie University, Halifax (Canada); Department of Physics and Atmospheric Science, Dalhousie University, Halifax (Canada); Thomas, Christopher [Department of Radiation Oncology, Dalhousie University, Halifax (Canada)

    2012-01-01

    This investigation focuses on possible dosimetric and efficiency advantages of HybridArc-a novel treatment planning approach combining optimized dynamic arcs with intensity-modulated radiation therapy (IMRT) beams. Application of this technique to two disparate sites, complex cranial tumors, and prostate was examined. HybridArc plans were compared with either dynamic conformal arc (DCA) or IMRT plans to determine whether HybridArc offers a synergy through combination of these 2 techniques. Plans were compared with regard to target volume dose conformity, target volume dose homogeneity, sparing of proximal organs at risk, normal tissue sparing, and monitor unit (MU) efficiency. For cranial cases, HybridArc produced significantly improved dose conformity compared with both DCA and IMRT but did not improve sparing of the brainstem or optic chiasm. For prostate cases, conformity was improved compared with DCA but not IMRT. Compared with IMRT, the dose homogeneity in the planning target volume was improved, and the maximum doses received by the bladder and rectum were reduced. Both arc-based techniques distribute peripheral dose over larger volumes of normal tissue compared with IMRT, whereas HybridArc involved slightly greater volumes of normal tissues compared with DCA. Compared with IMRT, cranial cases required 38% more MUs, whereas for prostate cases, MUs were reduced by 7%. For cranial cases, HybridArc improves dose conformity to the target. For prostate cases, dose conformity and homogeneity are improved compared with DCA and IMRT, respectively. Compared with IMRT, whether required MUs increase or decrease with HybridArc was site-dependent.

  13. A Hybrid Neural Network-Genetic Algorithm Technique for Aircraft Engine Performance Diagnostics

    Science.gov (United States)

    Kobayashi, Takahisa; Simon, Donald L.

    2001-01-01

    In this paper, a model-based diagnostic method, which utilizes Neural Networks and Genetic Algorithms, is investigated. Neural networks are applied to estimate the engine internal health, and Genetic Algorithms are applied for sensor bias detection and estimation. This hybrid approach takes advantage of the nonlinear estimation capability provided by neural networks while improving the robustness to measurement uncertainty through the application of Genetic Algorithms. The hybrid diagnostic technique also has the ability to rank multiple potential solutions for a given set of anomalous sensor measurements in order to reduce false alarms and missed detections. The performance of the hybrid diagnostic technique is evaluated through some case studies derived from a turbofan engine simulation. The results show this approach is promising for reliable diagnostics of aircraft engines.

  14. Hybrid inverse lithography techniques for advanced hierarchical memories

    Science.gov (United States)

    Xiao, Guangming; Hooker, Kevin; Irby, Dave; Zhang, Yunqiang; Ward, Brian; Cecil, Tom; Hall, Brett; Lee, Mindy; Kim, Dave; Lucas, Kevin

    2014-03-01

    Traditional segment-based model-based OPC methods have been the mainstream mask layout optimization techniques in volume production for memory and embedded memory devices for many device generations. These techniques have been continually optimized over time to meet the ever increasing difficulties of memory and memory periphery patterning. There are a range of difficult issues for patterning embedded memories successfully. These difficulties include the need for a very high level of symmetry and consistency (both within memory cells themselves and between cells) due to circuit effects such as noise margin requirements in SRAMs. Memory cells and access structures consume a large percentage of area in embedded devices so there is a very high return from shrinking the cell area as much as possible. This aggressive scaling leads to very difficult resolution, 2D CD control and process window requirements. Additionally, the range of interactions between mask synthesis corrections of neighboring areas can extend well beyond the size of the memory cell, making it difficult to fully take advantage of the inherent designed cell hierarchy in mask pattern optimization. This is especially true for non-traditional (i.e., less dependent on geometric rule) OPC/RET methods such as inverse lithography techniques (ILT) which inherently have more model-based decisions in their optimizations. New inverse methods such as model-based SRAF placement and ILT are, however, well known to have considerable benefits in finding flexible mask pattern solutions to improve process window, improve 2D CD control, and improve resolution in ultra-dense memory patterns. They also are known to reduce recipe complexity and provide native MRC compliant mask pattern solutions. Unfortunately, ILT is also known to be several times slower than traditional OPC methods due to the increased computational lithographic optimizations it performs. In this paper, we describe and present results for a methodology to

  15. A Robust Hybrid Video Watermarking Technique using Mosaicing

    Directory of Open Access Journals (Sweden)

    Jeebananda Panda

    2014-10-01

    Full Text Available Video Watermarking is the technique by which some information is inserted in the video which can be extracted later. It provides protection against any kind of illegal manipulation by third party. In this paper , a non -blind watermarking scheme is proposed which is based on the combination of Discrete Wavelet Transform,(DWT, Discrete Cosine Transform (DCT and Singular Value Decomposition (SVD.The watermark is embedded in the mosaic formed from the frames of the video by modifying the DCT coefficients of LL1 (low frequency sub-band obtained by applying 1-level DWT on the mosaic followed by diagonal based modification of singular value matrix which is obtained by SVD decomposition of LL2 (low frequency sub-band obtained by applying 2-level DWT on the modified LL1.The experimental values of PSNR, Correlation Factor show that the above proposed scheme is imperceptible, secure and robust against various types of attacks.

  16. Human disturbance causes the formation of a hybrid swarm between two naturally sympatric fish species.

    Science.gov (United States)

    Hasselman, Daniel J; Argo, Emily E; McBride, Meghan C; Bentzen, Paul; Schultz, Thomas F; Perez-Umphrey, Anna A; Palkovacs, Eric P

    2014-03-01

    Most evidence for hybrid swarm formation stemming from anthropogenic habitat disturbance comes from the breakdown of reproductive isolation between incipient species, or introgression between allopatric species following secondary contact. Human impacts on hybridization between divergent species that naturally occur in sympatry have received considerably less attention. Theory predicts that reinforcement should act to preserve reproductive isolation under such circumstances, potentially making reproductive barriers resistant to human habitat alteration. Using 15 microsatellites, we examined hybridization between sympatric populations of alewife (Alosa pseudoharengus) and blueback herring (A. aestivalis) to test whether the frequency of hybridization and pattern of introgression have been impacted by the construction of a dam that isolated formerly anadromous populations of both species in a landlocked freshwater reservoir. The frequency of hybridization and pattern of introgression differed markedly between anadromous and landlocked populations. The rangewide frequency of hybridization among anadromous populations was generally 0-8%, whereas all landlocked individuals were hybrids. Although neutral introgression was observed among anadromous hybrids, directional introgression leading to increased prevalence of alewife genotypes was detected among landlocked hybrids. We demonstrate that habitat alteration can lead to hybrid swarm formation between divergent species that naturally occur sympatrically, and provide empirical evidence that reinforcement does not always sustain reproductive isolation under such circumstances.

  17. High performance technique for database applicationsusing a hybrid GPU/CPU platform

    KAUST Repository

    Zidan, Mohammed A.

    2012-07-28

    Many database applications, such as sequence comparing, sequence searching, and sequence matching, etc, process large database sequences. we introduce a novel and efficient technique to improve the performance of database applica- tions by using a Hybrid GPU/CPU platform. In particular, our technique solves the problem of the low efficiency result- ing from running short-length sequences in a database on a GPU. To verify our technique, we applied it to the widely used Smith-Waterman algorithm. The experimental results show that our Hybrid GPU/CPU technique improves the average performance by a factor of 2.2, and improves the peak performance by a factor of 2.8 when compared to earlier implementations. Copyright © 2011 by ASME.

  18. Rapid detection of parasite in muscle fibers of fishes using a portable microscope imaging technique (Conference Presentation)

    Science.gov (United States)

    Lee, Jayoung; Lee, Hoonsoo; Kim, Moon S.; Cho, Byoungkwan

    2017-05-01

    Fishes are a widely used food material in the world. Recently about 4% of the fishes are infected with Kudoa thyrsites in Asian ocean. Kudoa thyrsites is a parasite that is found within the muscle fibers of fishes. The infected fishes can be a reason of food poisoning, which should be sorted out before distribution and consumption. Although Kudoa thyrsites is visible to the naked eye, it could be easily overlooked due to the micro-scale size and similar color with fish tissue. In addition, the visual inspection is labor intensive works resulting in loss of money and time. In this study, a portable microscopic camera was utilized to obtain images of raw fish slices. The optimized image processing techniques with polarized transmittance images provided reliable performance. The result shows that the portable microscopic imaging method can be used to detect parasites rapidly and non-destructively, which could be an alternative to manual inspections.

  19. A Proposed Hybrid Technique for Recognizing Arabic Characters

    Directory of Open Access Journals (Sweden)

    S F Bahgat

    2012-07-01

    Full Text Available Optical character recognition systems improve human-machine interaction and are urgently required for many governmental and commercial departments. A considerable progress in the recognition techniques of Latin and Chinese characters has been achieved. By contrast, Arabic Optical Character Recognition (AOCR is still lagging although the interest and research in this area is becoming more intensive than before. This is because the Arabic is a cursive language, written from right to left, each character has two to four different forms according to its position in the word, and most characters are associated with complementary parts above, below, or inside the character. The process of Arabic character recognition passes through several stages; the most serious and error-prone of which are segmentation, and feature extraction & classification. This research focuses on the feature extraction and classification stage, being as important as the segmentation stage. Features can be classified into two categories; Local features, which are usually geometric, and Global features, which are either topological or statistical. Four approaches related to the statistical category are to be investigated, namely: Moment Invariants, Gray Level Co-occurrence Matrix, Run Length Matrix, and Statistical Properties of Intensity Histogram. The paper aims at fusing the features of these methods to get the most representative feature vector that maximizes the recognition rate.

  20. On the Translation of Hybridity in the Chinese Version of Saving Fish from Drowning in the Light of Bakhtin’s Theory of Dialogue

    Institute of Scientific and Technical Information of China (English)

    HU Zheng-yan; WANG Ji-ping

    2015-01-01

    In translation, hybridity often poses a big challenge to the translator, especially when the hybrid text is a novel. The au⁃thor of this paper hopes to contribute to the study of the translation of hybridity in English novels by drawing on Bakhtin ’s theory of dialogue. By analyzing the translation of linguistic hybridity in Saving Fish from Drowning, the author of this paper finds that the translation by Caiju is not good enough and tries to provide a new direction by virtue of which translators can choose right transla⁃tion strategies.

  1. Designing a mathematical model of management techniques (TQM, BPR in Zahedan , weave fishing net industries

    Directory of Open Access Journals (Sweden)

    Dr.Baqer Kord, Dr. Habibollah Salarzehi, Hamed Aramesh, Somayeh Mousavi

    2010-10-01

    Full Text Available Currently reengineering and Total Quality Management (TQM techniques known improvement in organizations. in this research initially a Mathematical model was designed to find out the main factors in relative reengineering and TQM. Based on finding factors of Model, a 40 element questionary formed and the questionnaire distributed among the staff of fishing net factory in a random order, finding by using of SPSS, the data analyzed and concluding Remark shows the installation and acceptance of TQM by using reengineering is possible to the factory.

  2. Locomotion training of legged robots using hybrid machine learning techniques

    Science.gov (United States)

    Simon, William E.; Doerschuk, Peggy I.; Zhang, Wen-Ran; Li, Andrew L.

    1995-01-01

    In this study artificial neural networks and fuzzy logic are used to control the jumping behavior of a three-link uniped robot. The biped locomotion control problem is an increment of the uniped locomotion control. Study of legged locomotion dynamics indicates that a hierarchical controller is required to control the behavior of a legged robot. A structured control strategy is suggested which includes navigator, motion planner, biped coordinator and uniped controllers. A three-link uniped robot simulation is developed to be used as the plant. Neurocontrollers were trained both online and offline. In the case of on-line training, a reinforcement learning technique was used to train the neurocontroller to make the robot jump to a specified height. After several hundred iterations of training, the plant output achieved an accuracy of 7.4%. However, when jump distance and body angular momentum were also included in the control objectives, training time became impractically long. In the case of off-line training, a three-layered backpropagation (BP) network was first used with three inputs, three outputs and 15 to 40 hidden nodes. Pre-generated data were presented to the network with a learning rate as low as 0.003 in order to reach convergence. The low learning rate required for convergence resulted in a very slow training process which took weeks to learn 460 examples. After training, performance of the neurocontroller was rather poor. Consequently, the BP network was replaced by a Cerebeller Model Articulation Controller (CMAC) network. Subsequent experiments described in this document show that the CMAC network is more suitable to the solution of uniped locomotion control problems in terms of both learning efficiency and performance. A new approach is introduced in this report, viz., a self-organizing multiagent cerebeller model for fuzzy-neural control of uniped locomotion is suggested to improve training efficiency. This is currently being evaluated for a possible

  3. GA and PSO culled hybrid technique for economic dispatch problem with prohibited operating zones

    Institute of Scientific and Technical Information of China (English)

    SUDHAKARAN M.; AJAY-D-VIMALRAJ P.; PALANIVELU T.G.

    2007-01-01

    This paper presents an efficient and reliable genetic algorithm (GA) based particle swarm optimization (PSO) technique (hybrid GAPSO) for solving the economic dispatch (ED) problem in power systems. The non-linear characteristics of the generators, such as prohibited operating zones, ramp rate limits and non-smooth cost functions of the practical generator operation are considered. The proposed hybrid algorithm is demonstrated for three different systems and the performance is compared with the GA and PSO in terms of solution quality and computation efficiency. Comparison of results proved that the proposed algorithm can obtain higher quality solutions efficiently in ED problems. A comprehensive software package is developed using MATLAB.

  4. Fish predation on a Daphnia hybrid species complex: A factor explaining species coexistence?

    NARCIS (Netherlands)

    Spaak, P.; Hoekstra, J.F.

    1997-01-01

    Recent studies on the life histories of Daphnia hybrids and their parental species have revealed that hybrids can combine an intermediate size with a relatively high reproductive rate, which might explain their success in many European lakes. Based on this information, we formulated the temporal

  5. Indirect porcelain veneers in periodontally compromised teeth. The hybrid technique: a case report.

    Science.gov (United States)

    Garcia-Baeza, David; Saavedra, Carlos; Garcia-Adámez, Ramón

    2015-01-01

    The loss of periodontal structure causes an esthetic problem for many patients, especially when the esthetic zone is compromised. Among the various types of solutions is the use of composite resins. While this procedure is not aggressive towards tooth structure, it does require the clinician to have a precise technique, and demands strict longterm maintenance. 1 Another way of treating the compromised teeth is with porcelain veneers. This procedure is especially difficult, however, if carried out on periodontal teeth, as it requires preparation along the roots. 2 The intention of the hybrid technique described in this article is to combine both of these procedures in order to obtain a less aggressive treatment with precise management of the soft tissue and an adequate esthetic outcome. The hybrid technique consists of enlarging the root portion of the teeth with composite resin to obtain a less aggressive tooth preparation, and thereafter placing porcelain veneers.

  6. Fully automated fluorescent in situ hybridization (FISH staining and digital analysis of HER2 in breast cancer: a validation study.

    Directory of Open Access Journals (Sweden)

    Elise M J van der Logt

    Full Text Available HER2 assessment is routinely used to select patients with invasive breast cancer that might benefit from HER2-targeted therapy. The aim of this study was to validate a fully automated in situ hybridization (ISH procedure that combines the automated Leica HER2 fluorescent ISH system for Bond with supervised automated analysis with the Visia imaging D-Sight digital imaging platform. HER2 assessment was performed on 328 formalin-fixed/paraffin-embedded invasive breast cancer tumors on tissue microarrays (TMA and 100 (50 selected IHC 2+ and 50 random IHC scores full-sized slides of resections/biopsies obtained for diagnostic purposes previously. For digital analysis slides were pre-screened at 20x and 100x magnification for all fluorescent signals and supervised-automated scoring was performed on at least two pictures (in total at least 20 nuclei were counted with the D-Sight HER2 FISH analysis module by two observers independently. Results were compared to data obtained previously with the manual Abbott FISH test. The overall agreement with Abbott FISH data among TMA samples and 50 selected IHC 2+ cases was 98.8% (κ = 0.94 and 93.8% (κ = 0.88, respectively. The results of 50 additionally tested unselected IHC cases were concordant with previously obtained IHC and/or FISH data. The combination of the Leica FISH system with the D-Sight digital imaging platform is a feasible method for HER2 assessment in routine clinical practice for patients with invasive breast cancer.

  7. 3D Printed PEG-Based Hybrid Nanocomposites Obtained by Sol-Gel Technique.

    Science.gov (United States)

    Chiappone, Annalisa; Fantino, Erika; Roppolo, Ignazio; Lorusso, Massimo; Manfredi, Diego; Fino, Paolo; Pirri, Candido Fabrizio; Calignano, Flaviana

    2016-03-02

    In this work, three-dimensional (3D) structured hybrid materials were fabricated combining 3D printing technology with in situ generation of inorganic nanoparticles by sol-gel technique. Those materials, consisting of silica nanodomains covalently interconnected with organic polymers, were 3D printed in complex multilayered architectures, incorporating liquid silica precursors into a photocurable oligomer in the presence of suitable photoinitiators and exposing them to a digital light system. A post sol-gel treatment in acidic vapors allowed the in situ generation of the inorganic phase in a dedicated step. This method allows to build hybrid structures operating with a full liquid formulation without meeting the drawbacks of incorporating inorganic powders into 3D printable formulations. The influence of the generated silica nanoparticle on the printed objects was deeply investigated at macro- and nanoscale; the resulting light hybrid structures show improved mechanical properties and, thus, have a huge potential for applications in a variety of advanced technologies.

  8. Closure technique for the hybrid girder cable stayed bridge of Edong Bridge

    Institute of Scientific and Technical Information of China (English)

    Liu Minghu; Tan Hao; Xu GuoPing; Zhao Canhui

    2012-01-01

    Based on Edong Yangtze River Bridge, which is the second longest hybrid girder cable stayed bridge with 926 m long main span, the influencing factors and crucial techniques of the main span closure method for long span hybrid girder cable stayed bridge are studied. After theoretical analysis, numerical evaluation and practical test, the loading assistant closure method is employed in Edong Yangtze River Bridge. The loading assistant closure method, with better thermal adaptability and less influence on bridge line and the forced status, can meet the requirements of the unstressed state control method. Based on the mentioned advantages, the loading assistant closure method is applicable to long span hybrid girder cable stayed bridges. The conclusion can provide a reference for the further design of the similar brid~es.

  9. A new hybrid jpeg image compression scheme using symbol reduction technique

    CERN Document Server

    Kumar, Bheshaj; Sinha, G R

    2012-01-01

    Lossy JPEG compression is a widely used compression technique. Normally the JPEG standard technique uses three process mapping reduces interpixel redundancy, quantization, which is lossy process and entropy encoding, which is considered lossless process. In this paper, a new technique has been proposed by combining the JPEG algorithm and Symbol Reduction Huffman technique for achieving more compression ratio. The symbols reduction technique reduces the number of symbols by combining together to form a new symbol. As a result of this technique the number of Huffman code to be generated also reduced. It is simple fast and easy to implement. The result shows that the performance of standard JPEG method can be improved by proposed method. This hybrid approach achieves about 20% more compression ratio than the Standard JPEG.

  10. Hybrid single-beam reconstruction technique for slow and fast varying wave fields.

    Science.gov (United States)

    Falaggis, Konstantinos; Kozacki, Tomasz; Kujawinska, Malgorzata

    2015-06-01

    An iterative single-beam wave field reconstruction technique that employs both non-paraxial, wave propagation based and paraxial deterministic phase retrieval techniques is presented. This approach overcomes two major obstacles that exist in the current state of the art techniques: iterative methods do not reconstruct slowly varying wave fields due to slow convergence and stagnation, and deterministic methods have paraxial limits, making the reconstructions of quickly varying object features impossible. In this work, a hybrid approach is reported that uses paraxial wave field corrections within iterative phase retrieval solvers. This technique is suitable for cases ranging from slow to fast varying wave fields, and unlike the currently available methods, can also reconstruct measurement objects with different regions of both slowly and quickly varying object features. It is further shown that this technique gives a higher accuracy than current single-beam phase retrieval techniques, and in comparison to the iterative methods, has a higher convergence speed.

  11. Action spectrum for melanoma induction in hybrid fish of the genus Xiphophorus

    Energy Technology Data Exchange (ETDEWEB)

    Setlow, R.B.

    1997-03-01

    Cutaneous malignant melanoma (CMM) is a complicated disease that is dependent on a number of factors that influence its incidence in ways that are quantitatively uncertain. The incidence of CMM increases with proximity to the Equator -- an observation in line with the conclusion that sun exposure is the most important etiologic agent. However, the latitude effect does not implicate UVB because the intensities of all spectral regions increase toward the Equator. An understanding of the useful public health measures to lower the incidence of CMM would benefit greatly if the spectral region of sunlight implicated in melanoma incidence were known. Such knowledge requires animal models to evaluate the incidence as a function of wavelength. There are marsupial models, a transgenic mouse model, and a fish model. To date, only the fish model has been employed to obtain an action spectrum. The paper describes a fish model, implications of the fish spectrum, and epidemiological data.

  12. In vitro formation and thermal transition of novel hybrid fibrils from type I fish scale collagen and type I porcine collagen

    OpenAIRE

    Song Chen, Toshiyuki Ikoma, Nobuhiro Ogawa, Satoshi Migita, Hisatoshi Kobayashi and Nobutaka Hanagata

    2010-01-01

    Novel type I collagen hybrid fibrils were fabricated by neutralizing a mixture of type I fish scale collagen solution and type I porcine collagen solution with a phosphate buffer saline at 28 °C. Their structure was discussed in terms of the volume ratio of fish/porcine collagen solution. Scanning electron and atomic force micrographs showed that the diameter of collagen fibrils derived from the collagen mixture was larger than those derived from each collagen, and all resultant fibrils exhib...

  13. The applications of fluorescence in situ hybridization technique in tumors of the genitourinary%荧光原位杂交技术在泌尿生殖肿瘤中的应用

    Institute of Scientific and Technical Information of China (English)

    袁振; 蒋雷鸣

    2012-01-01

    随着分子细胞遗传学的快速发展,荧光原位杂交(fluorescence in situ hybridization,FISH)技术已广泛应用于临床和科学研究.FISH技术综合了荧光信号的高度灵敏性、无放射性、直观性及原位杂交的准确性,通过检测样本中基因或染色体的异常改变而应用于遗传性疾病、肿瘤的研究及临床诊断和治疗监测.本文就荧光原位杂交技术在常见的泌尿生殖肿瘤中的应用进行综述.%With the rapid development of molecular cytogenetics, fluorescence in situ hybridization ( FISH ) technique has been widely used in clinical and research. FISH has the high sensitivity of fluorescence signal, non - radioactive , intuitive, and the accuracy of in situ hybridization, can be used in detecting the abnormal gene or chromosome change of genetic diseases, cancer research and clinical diagnosis and treatment monitoring. In this paper, the applications of fluorescence in situ hybridization technique in diagnosis and treatment of common urinary tract cancer are reviewed.

  14. Sample preparation and in situ hybridization techniques for automated molecular cytogenetic analysis of white blood cells

    Energy Technology Data Exchange (ETDEWEB)

    Rijke, F.M. van de; Vrolijk, H.; Sloos, W. [Leiden Univ. (Netherlands)] [and others

    1996-06-01

    With the advent in situ hybridization techniques for the analysis of chromosome copy number or structure in interphase cells, the diagnostic and prognostic potential of cytogenetics has been augmented considerably. In theory, the strategies for detection of cytogenetically aberrant cells by in situ hybridization are simple and straightforward. In practice, however, they are fallible, because false classification of hybridization spot number or patterns occurs. When a decision has to be made on molecular cytogenetic normalcy or abnormalcy of a cell sample, the problem of false classification becomes particularly prominent if the fraction of aberrant cells is relatively small. In such mosaic situations, often > 200 cells have to be evaluated to reach a statistical sound figure. The manual enumeration of in situ hybridization spots in many cells in many patient samples is tedious. Assistance in the evaluation process by automation of microscope functions and image analysis techniques is, therefore, strongly indicated. Next to research and development of microscope hardware, camera technology, and image analysis, the optimization of the specimen for the (semi)automated microscopic analysis is essential, since factors such as cell density, thickness, and overlap have dramatic influences on the speed and complexity of the analysis process. Here we describe experiments that have led to a protocol for blood cell specimen that results in microscope preparations that are well suited for automated molecular cytogenetic analysis. 13 refs., 4 figs., 1 tab.

  15. Biomechanical comparison of interference screw and cortical button with screw hybrid technique for distal biceps brachii tendon repair.

    Science.gov (United States)

    Arianjam, Afshin; Camisa, William; Leasure, Jeremi M; Montgomery, William H

    2013-11-01

    Various fixation techniques have been described for ruptured distal biceps tendons. The authors hypothesized that no significant differences would be found between the mean failure strength, maximum strength, and stiffness of the interference screw and hybrid technique. Fourteen fresh-frozen human cadaveric elbows were prepared. Specimens were randomized to either interference screw or hybrid cortical button with screw fixation. The tendon was pulled at a rate of 4 mm/s until failure. Failure strength, maximum strength, and stiffness were measured and compared. Failure strength, maximum strength, and stiffness were 294±81.9 N, 294±82.1 N, and 64.4±40.5 N/mm, respectively, for the interference screw technique and 333±129 N, 383±121 N, and 56.2±40.5 N/mm, respectively, for the hybrid technique. No statistically significant difference existed between the screw and hybrid technique in failure strength, maximum strength, or stiffness (P>.05). The interference screws primarily failed by pullout of the screw and tendon, whereas in the hybrid technique, failure occurred with screw pullout followed by tearing of the biceps tendon. The results suggest that this hybrid technique is nearly as strong and stiff as the interference screw alone. Although the hybrid technique facilitates tensioning of the reconstructed tendon, the addition of the cortical button did not significantly improve the failure strength of the interference screw alone. Copyright 2013, SLACK Incorporated.

  16. Prediction of melting temperatures in fluorescence in situ hybridization (FISH) procedures using thermodynamic models

    DEFF Research Database (Denmark)

    Fontenete, Sílvia; Guimarães, Nuno; Wengel, Jesper

    2016-01-01

    Abstract The thermodynamics and kinetics of DNA hybridization, i.e. the process of self-assembly of one, two or more complementary nucleic acid strands, has been studied for many years. The appearance of the nearest-neighbor model led to several theoretical and experimental papers on DNA thermody......Abstract The thermodynamics and kinetics of DNA hybridization, i.e. the process of self-assembly of one, two or more complementary nucleic acid strands, has been studied for many years. The appearance of the nearest-neighbor model led to several theoretical and experimental papers on DNA...... thermodynamics that provide reasonably accurate thermodynamic information on nucleic acid duplexes and allow estimation of the melting temperature. Because there are no thermodynamic models specifically developed to predict the hybridization temperature of a probe used in a fluorescence in situ hybridization...

  17. A DG Implementation of a Novel Hybrid RANS/LES Technique With RANS Reconstruction

    CERN Document Server

    Abbà, Antonella; Nini, Michele; Restelli, Marco

    2014-01-01

    A new hybrid RANS/LES technique, based on the hybrid filter proposed by Germano in 2004, has been studied. The novelty herein introduced is represented by the reconstruction of the Reynolds stress tensor. As a consequence, no explicit RANS model is needed. The RANS and LES terms are merged using a constant blending factor. The model is implemented in a numerical code based on a high order Discontinuous Galerkin (DG) finite element formulation. The test case considered for numerical simulations is the turbulent tur- bulent channel flow at Mach = 0.2. The comparison with available DNS data shows a good agreement and, in general, an improvement with re- spect to pure LES results, confirming that the technique herein proposed represents a promising approach to the numerical simulation of turbulent flows.

  18. [Application of FISH-NanoSIMS technique in environmental microbial ecology study].

    Science.gov (United States)

    Chen, Chen; Bai, Yao-hui; Liang, Jin-song; Yuan, Lin-jiang

    2015-01-01

    With the development of microbial ecology techniques, it is possible to analyze the distribution and function of microorganisms simultaneously in complex ecosystems. To explore the application of FISH-NanoSIMS in environmental microbial ecology study, our study used the stable isotope labeled compounds 13C-C6H12O6, and 15N-NH4Cl as C and N sources for cultivating the pure culture (manganese oxidizing bacteria, Pseudomonas sp. QJX-1) and environmental samples (the shallow soil and anaerobic sludge). FISH-NanoSIMS was used to detect the distribution of microorganisms and relatively quantify secondary ions (12C-, 13C-, 12C(14)N-, 12C15N-) in cultivated samples, in order to explore the utilization of C and N isotopes sources by the pure culture and microorganisms in environment samples. The results showed that the contents of 13C and 5N in the area of bacteria were significantly greater than the natural abundance in all samples. It indicated that Pseudomonas sp. QJX-1 and some specific bacteria in environmental samples could metabolize 13C-C6H12O6 and 15N-NH4C1. Furthermore, this study revealed that for Pseudomonas sp. QJX-1, the manganese oxidation only occurred when the carbon and nitrogen were consumed to a low level. For environmental samples, the bacterial nitrification and denitrification were both observed in the shallow soil and anaerobic sludge. In a word, our study demonstrated that the combination of FISH and NanoSIMS could simultaneously examine microbial distribution and microbial metabolic activity in environmental samples, which will help us to obtain the eco-physiology information of microbial community.

  19. Protocols for Pachytene Chromosome and DNA Fiber FISH in Cotton

    Institute of Scientific and Technical Information of China (English)

    PENG Ren-hai; LING Jian; WANG Kun-bo; WANG Chun-ying; SONG Guo-li; LIU Fang

    2008-01-01

    @@ Fluorescence in situ hybridization (FISH) has become the most important technique in plant molecular cytogenetics research.FISH-based physical mapping provides a valuable complementary approach in genome sequeneing to measure the physical distances between adjacent BAC contigs and delineating the structure and DNA composition of genomic regions of centromere and telomere.The accuracy and precision of the FISH-base physical maps depend on the resolution power of FISH.

  20. Assessment of chromosomal abnormalities in sperm of infertile men using sperm karyotyping and multicolour fluorescence in situ hybridization (FISH)

    Energy Technology Data Exchange (ETDEWEB)

    Moosani, N.; Martin, R.H. [Alberta Children`s Hospital and Univ. of Calgary (Canada)

    1994-09-01

    Individuals with male factor infertility resulting from idiopathic oligo-, astheno- or teratozoospermia are frequently offered IVF in an attempt to increase their chances of having a child. A concern remains whether these infertile males have an elevated risk of transmitting chromosomal abnormalities to their offspring. Sperm chromosomal complements from these men were assayed using the human sperm/hamster oocyte fusion system and fluorescence in situ hybridization (FISH) on sperm nuclei. For each of 5 infertile patients, 100 sperm karyotypes were analyzed and multicolour FISH analysis was performed on a minimum of 10,000 sperm nuclei for each chromosome-specific DNA probe for chromosomes 1 (pUC1.77), 12 (D12Z3), X (XC) and Y (DYZ3). As a group, the infertile patients showed increased frequencies of both numerical ({chi}{sup 2}=17.26, {proportional_to} <0.001) and total abnormalities ({chi}{sup 2}=7.78, {proportional_to} <0.01) relative to control donors when assessed by sperm karyotypes. Analysis of sperm nuclei by FISH indicated a significant increase in the frequency of disomy for chromosome 1 in three of the five patients as compared to control donors ({chi}{sup 2}>8.35, {proportional_to} <0.005). In addition, the frequency of XY disomy was significantly higher in four of the five patients studied by FISH ({chi}{sup 2}>10.58, {proportional_to}<0.005), suggesting that mis-segregation caused by the failure of the XY bivalent to pair may play a role in idiopathic male infertility.

  1. A low-loss hybrid rectification technique for piezoelectric energy harvesting

    Science.gov (United States)

    Schlichting, A. D.; Fink, E.; Garcia, E.

    2013-09-01

    Embedded systems have decreased in size and increased in capability; however, small-scale energy storage technologies still significantly limit these advances. Energy neutral operation using small-scale energy harvesting technologies would allow for longer device operation times and smaller energy storage masses. Vibration energy harvesting is an attractive method due to the prevalence of energy sources in many environments. Losses in efficiency due to AC-DC rectification and conditioning circuits limit its application. This work presents a low-loss hybrid rectification technique for piezoelectric vibration energy harvesting using magnetically actuated reed switches and a passive semiconductor full-bridge rectifier. This method shows the capability to have higher efficiency levels and the rectification of low-voltage harvesters without the need for active electrical components. A theoretical model shows that the hybrid rectification technique performance is highly dependent on the proximity delay and the hysteresis behavior of the reed switches. Experimental results validate the model and support the hypothesis of increased performance using the hybrid rectification technique.

  2. A Comparison of Redundancy Techniques for Private and Hybrid Cloud Storage

    Directory of Open Access Journals (Sweden)

    E.M. Hernandez-Ramirez

    2012-11-01

    Full Text Available File redundancy techniques have been very useful mechanisms for offering fault tolerance and data availability in anykind of storage. Cloud storage is not the exception. This paper presents an evaluation of classical file redundancytechniques implemented in two cloud-storage deployment models, private and hybrid. A small prototype of a privateand hybrid cloud storage was implemented for this evaluation. The performance impact when file redundancy is onlyapplied in a private cloud versus when redundancy is also distributed in a public cloud (the hybrid model is analyzed.Additional to classical file redundancy techniques, an innovative method was evaluated for file redundancy based onan information dispersal algorithm (IDA. The usage of IDA represents a good option for managing sensitive data inhybrid cloud storage. In this technique, only parts of a file need to be sent to the public cloud, avoiding the completefile to be read from outside of the private zone. In this context, there is a trade-off between performance (forreconstructing the original file, it is first necessary to obtain all of its fragments and the security level that coulddetermine the viability of using IDA.

  3. High capacity fiber optic sensor networks using hybrid multiplexing techniques and their applications

    Science.gov (United States)

    Sun, Qizhen; Li, Xiaolei; Zhang, Manliang; Liu, Qi; Liu, Hai; Liu, Deming

    2013-12-01

    Fiber optic sensor network is the development trend of fiber senor technologies and industries. In this paper, I will discuss recent research progress on high capacity fiber sensor networks with hybrid multiplexing techniques and their applications in the fields of security monitoring, environment monitoring, Smart eHome, etc. Firstly, I will present the architecture of hybrid multiplexing sensor passive optical network (HSPON), and the key technologies for integrated access and intelligent management of massive fiber sensor units. Two typical hybrid WDM/TDM fiber sensor networks for perimeter intrusion monitor and cultural relics security are introduced. Secondly, we propose the concept of "Microstructure-Optical X Domin Refecltor (M-OXDR)" for fiber sensor network expansion. By fabricating smart micro-structures with the ability of multidimensional encoded and low insertion loss along the fiber, the fiber sensor network of simple structure and huge capacity more than one thousand could be achieved. Assisted by the WDM/TDM and WDM/FDM decoding methods respectively, we built the verification systems for long-haul and real-time temperature sensing. Finally, I will show the high capacity and flexible fiber sensor network with IPv6 protocol based hybrid fiber/wireless access. By developing the fiber optic sensor with embedded IPv6 protocol conversion module and IPv6 router, huge amounts of fiber optic sensor nodes can be uniquely addressed. Meanwhile, various sensing information could be integrated and accessed to the Next Generation Internet.

  4. A HYBRID TECHNIQUE FOR FREQUENCY DOMAIN IDENTIFICATION OF SERVO SYSTEM WITH FRICTION FORCE

    Directory of Open Access Journals (Sweden)

    SHAIK.RAFI KIRAN,

    2011-03-01

    Full Text Available The system identification process in servo system with frictional force seems to be a complex task becauseof its non-linear nature. For such non-linear systems, a good choice is system identification in frequencydomain. However, most of the techniques are manual and are inappropriate for determination of systemparameters. This makes system identification ineffective for servo systems with frictional force. Toovercome this issue, a hybrid technique is proposed in this paper. The proposed technique exploits neuralnetwork and genetic algorithm to determine the system parameters of servo systems with friction. In theproposed technique, the target parameters are determined from the transfer function derived for thesystem. Subsequently, the system parameters are identified by a process formed by blending the neuralnetwork and genetic algorithm techniques. Prior to performing the identification procedure, backpropagation training is given to the neural network using a pre-examined dataset. Then with thecombined operation of neural network and genetic algorithm, the system parameters that are closer tothe target parameters for the servo system with frictional force are determined. The technique isimplemented and compared with the existing frequency domain identification technique. From thecomparative results, it is evident that the proposed technique outperforms the existing technique.

  5. Recent Advances in Fluorescence in situ Hybridization

    OpenAIRE

    吉田, 廸弘; Michihiro C., Yoshida; 北海道大学理学部附属動物染色体研究施設; Chromosome Research Unit, Faculty of Science, Hokkaido University

    1992-01-01

    Fluorescence in situ hybridization (FISH) procedures that directly couple molecular and cytological information allow precise visualization of DNA sequences on metaphase chromosomes and interphase nuclei. These techniques can be used to identify chromosomes, detect chromosomal aberrations, and analyze linear and spetial genome organization. FISH procedures are also used to clinical fields for diagnosis of disease-related chromosome changes and tumor biology.

  6. Gill monogeneans of Nile tilapia (Oreochromis niloticus) and red hybrid tilapia (Oreochromis spp.) from the wild and fish farms in Perak, Malaysia: infection dynamics and spatial distribution.

    Science.gov (United States)

    Lim, Shen-Yin; Ooi, Ai-Lin; Wong, Wey-Lim

    2016-01-01

    Tilapia is one of the commercially important fish in Malaysia as well as in other parts of the world. An understanding of monogenean infection dynamics in tilapia fish may assist us in searching for some intervention measures in reducing the loss of fish caused by parasitic diseases. The present study aimed (1) to compare infection level of monogeneans between the wild and cultured Oreochromis niloticus, and between the cultured O. niloticus and cultured red hybrid tilapia, and (2) to examine the spatial distribution of monogenean species over the gills of the different host species. From a total of 75 fish specimens, six species of monogeneans from two genera: Cichlidogyrus (C. halli, C. mbirizei, C. sclerosus, C. thurstonae, C. tilapiae) and Scutogyrus (S. longicornis) were identified. Data showed that the infection level of cultured O. niloticus was higher than that of the wild O. niloticus, however, the former was lower than that of the cultured red hybrid tilapia. Higher species richness of monogeneans was observed in the cultured red hybrid tilapia as compared to the others. Results for spatial distribution showed that the monogeneans have no preference on the left or right sides of the gills. However, C. halli, C. mbirizei, and C. tilapiae showed preferences on specific gill arches in the cultured O. niloticus and red hybrid tilapia. In general, the gill arch IV harboured the least number of monogeneans. The susceptibility of monogenean infection between the different types of tilapia is discussed.

  7. Phylogeny-function analysis of (meta)genomic libraries: screening for expression of ribosomal RNA genes by large-insert library fluorescent in situ hybridization (LIL-FISH)

    NARCIS (Netherlands)

    Leveau, J.H.J.; Gerards, S.; De Boer, W.; Van Veen, J.A.

    2004-01-01

    We assessed the utility of fluorescent in situ hybridization (FISH) in the screening of clone libraries of (meta)genomic or environmental DNA for the presence and expression of bacterial ribosomal RNA (rRNA) genes. To establish proof-of-principle, we constructed a fosmid-based library in Escherichia

  8. Temperature controlling system based on FISH probe hybridization facility%基于FISH探针杂交设备的温度控制系统

    Institute of Scientific and Technical Information of China (English)

    李天庆; 张化; 张婉尧

    2015-01-01

    以玻片为基础的FISH,它的变性杂交过程完全实现自动化.FISH探针杂交仪内置一个ARM7微处理器,以控制不同温度变化,满足变性、杂交和固定温度等不同模式,可预置用按键编程进去的程序(显示屏上能指导编程),方便客户调用,提高工作效率.温度控制系统是FISH探针杂交设备的核心部分,决定了杂交仪的可靠性、稳定性及精准性.%By using FISH based on slide,automation in the degeneration process of hybrid can be achieved.Installed an internal ARM7 microprocessor,the FISH probe hybridization facility can control temperature,meet different modes such as denaturation,hybridization,fixed temperature,and so on.Procedure input by means of push-button can be preset(there are guidance on display screen)to make things easy for user,as a result,work efficiency can be improved.The temperature controlling system,which is the core of FISH probe hybridization facility and ensure its reliability,stability and accuracy.

  9. Distribution characteristics of ammonia-oxidizing bacteria in the Typha latifolia constructed wetlands using fluorescent in situ hybridization (FISH).

    Science.gov (United States)

    Yan, Li; Inamori, Ryuhei; Gui, Ping; Xu, Kai-qin; Kong, Hai-nan; Matsumura, Masatoshi; Inamori, Yuhei

    2005-01-01

    A molecular biology method, fluorescent in situ hybridization (FISH), in which the pre-treatment was improved in allusion to the media of the constructed wetlands (CW), e.g. the soil and the grit, was used to investigate the vertical distribution characteristics of ammonia-oxidizing bacteria (AOB) quantity and the relation with oxidation-reduction potential (ORP) in the Typha latifolia constructed wetlands under three different loadings in summer from May to September. Results showed that the quantity of the AOB decreased in the Typha latifolia CW with the increase of vertical depth. However, the AOB quantity was 2-4 times the quantity of the control in the root area. Additionally, ORP in the rhizosphere was found to be higher than other areas, which showed that Typha latifolia CW was in an aerobic state in summer when using simulated non-point sewage at the rural area of Taihu Lake in China and small town combined sewage.

  10. Fishing technique of long-fingered bats was developed from a primary reaction to disappearing target stimuli

    DEFF Research Database (Denmark)

    Aizpurua, Ostaizka; Alberdi, Antton; Aihartza, Joxerra

    2016-01-01

    Behavioral plasticity is a key feature allowing animals to broaden their dietary niche when novel food resources become available, and long-fingered bats provide an appropriate model system to study the underpinnings of behavioral plasticity, since although generally being an insectivorous species...... that the fishing technique was developed from a primary reaction shared by all long-fingered bats. All individuals seem to be mechanically and sensorially adapted to detect and capture fish, although under appropriate environmental conditions, they would further improve their technique by experience and/or social...

  11. Hybrid striped bass feeds based on fish oil, beef tallow, and eicosapentaenoic acid/docosahexaenoic acid supplements: Insight regarding fish oil sparing and demand for -3 long-chain polyunsaturated fatty acids.

    Science.gov (United States)

    Bowzer, J; Jackson, C; Trushenski, J

    2016-03-01

    Previous research suggests that saturated (SFA) and monounsaturated fatty acid (MUFA) rich lipids, including beef tallow, can make utilization or diet-to-tissue transfer of long-chain polyunsaturated fatty acids (LC-PUFA) more efficient. We hypothesized that using beef tallow as an alternative to fish oil may effectively reduce the LC-PUFA demand of hybrid striped bass × and allow for greater fish oil sparing. Accordingly, we evaluated growth performance and tissue fatty acid profiles of juvenile fish (23.7 ± 0.3 g) fed diets containing menhaden fish oil (considered an ideal source of LC-PUFA for this taxon), beef tallow (BEEF ONLY), or beef tallow amended with purified sources of eicosapentaenoic acid (EPA) and/or docosahexaenoic acid (DHA) to achieve levels corresponding to 50 or 100% of those observed in the FISH ONLY feed. Diets were randomly assigned to quadruplicate tanks of fish ( = 4; 10 fish/tank), and fish were fed assigned diets to apparent satiation once daily for 10 wk. Survival (98-100%) was equivalent among treatments, but weight gain (117-180%), specific growth rate (1.1-1.5% BW/d), feed intake (1.4-1.8% BW/d), thermal growth coefficient (0.50-0.70), and feed conversion ratio (FCR; 1.1-1.4, DM basis) varied. Except for FCR, no differences were observed between the FISH ONLY and BEEF ONLY treatments, but performance was generally numerically superior among fish fed the diets containing beef tallow supplemented with DHA at the 100% or both EPA and DHA at the 50% or 100% level. Tissue fatty acid composition was significantly distorted in favor among fish fed the beef tallow-based feeds; however, profile distortion was most overt in peripheral tissues. Results suggest that beef tallow may be used as a primary lipid source in practical diets for hybrid striped bass, but performance may be improved by supplementation with LC-PUFA, particularly DHA. Furthermore, our results suggest that -3 LC-PUFA requirements reported for hybrid striped bass may not be

  12. Recent Developments on Hybrid Time-Frequency Numerical Simulation Techniques for RF and Microwave Applications

    Directory of Open Access Journals (Sweden)

    Jorge F. Oliveira

    2013-01-01

    Full Text Available This paper reviews some of the promising doors that functional analysis techniques have recently opened in the field of electronic circuit simulation. Because of the modulated nature of radio frequency (RF signals, the corresponding electronic circuits seem to operate in a slow time scale for the aperiodic information and another, much faster, time scale for the periodic carrier. This apparent multirate behavior can be appropriately described using partial differential equations (PDEs within a bivariate framework, which can be solved in an efficient way using hybrid time-frequency techniques. With these techniques, the aperiodic information dimension is treated in the discrete time domain, while the periodic carrier dimension is processed in the frequency domain, in which the solution is evaluated within a space of harmonically related sinusoidal functions. The objective of this paper is thus to provide a general overview on the most important hybrid time-frequency techniques, as the ones found in commercial tools or the ones recently published in the literature.

  13. In vitro formation and thermal transition of novel hybrid fibrils from type I fish scale collagen and type I porcine collagen

    Energy Technology Data Exchange (ETDEWEB)

    Chen Song; Ogawa, Nobuhiro; Migita, Satoshi; Kobayashi, Hisatoshi [Biomaterials Center, National Institute for Materials Science, 1-2-1 Sengen, Tsukuba, Ibaraki 305-0047 (Japan); Ikoma, Toshiyuki [Department of Metallurgy and Ceramics Science, Tokyo Institute of Technology, O-okayama 2-12-1, Meguro-ku, Tokyo 152-8550 (Japan); Hanagata, Nobutaka, E-mail: HANAGATA.Nobutaka@nims.go.j [Nanotechnology Innovation Center, National Institute for Materials Science, 1-2-1 Sengen, Tsukuba, Ibaraki 305-0047 (Japan)

    2010-06-15

    Novel type I collagen hybrid fibrils were fabricated by neutralizing a mixture of type I fish scale collagen solution and type I porcine collagen solution with a phosphate buffer saline at 28 {sup 0}C. Their structure was discussed in terms of the volume ratio of fish/porcine collagen solution. Scanning electron and atomic force micrographs showed that the diameter of collagen fibrils derived from the collagen mixture was larger than those derived from each collagen, and all resultant fibrils exhibited a typical D-periodic unit of {approx}67 nm, irrespective of volume ratio of both collagens. Differential scanning calorimetry revealed only one endothermic peak for the fibrils derived from collagen mixture or from each collagen solution, indicating that the resultant collagen fibrils were hybrids of type I fish scale collagen and type I porcine collagen.

  14. In vitro formation and thermal transition of novel hybrid fibrils from type I fish scale collagen and type I porcine collagen

    Science.gov (United States)

    Chen, Song; Ikoma, Toshiyuki; Ogawa, Nobuhiro; Migita, Satoshi; Kobayashi, Hisatoshi; Hanagata, Nobutaka

    2010-06-01

    Novel type I collagen hybrid fibrils were fabricated by neutralizing a mixture of type I fish scale collagen solution and type I porcine collagen solution with a phosphate buffer saline at 28 °C. Their structure was discussed in terms of the volume ratio of fish/porcine collagen solution. Scanning electron and atomic force micrographs showed that the diameter of collagen fibrils derived from the collagen mixture was larger than those derived from each collagen, and all resultant fibrils exhibited a typical D-periodic unit of ~67 nm, irrespective of volume ratio of both collagens. Differential scanning calorimetry revealed only one endothermic peak for the fibrils derived from collagen mixture or from each collagen solution, indicating that the resultant collagen fibrils were hybrids of type I fish scale collagen and type I porcine collagen.

  15. In vitro formation and thermal transition of novel hybrid fibrils from type I fish scale collagen and type I porcine collagen

    Directory of Open Access Journals (Sweden)

    Song Chen, Toshiyuki Ikoma, Nobuhiro Ogawa, Satoshi Migita, Hisatoshi Kobayashi and Nobutaka Hanagata

    2010-01-01

    Full Text Available Novel type I collagen hybrid fibrils were fabricated by neutralizing a mixture of type I fish scale collagen solution and type I porcine collagen solution with a phosphate buffer saline at 28 °C. Their structure was discussed in terms of the volume ratio of fish/porcine collagen solution. Scanning electron and atomic force micrographs showed that the diameter of collagen fibrils derived from the collagen mixture was larger than those derived from each collagen, and all resultant fibrils exhibited a typical D-periodic unit of ~67 nm, irrespective of volume ratio of both collagens. Differential scanning calorimetry revealed only one endothermic peak for the fibrils derived from collagen mixture or from each collagen solution, indicating that the resultant collagen fibrils were hybrids of type I fish scale collagen and type I porcine collagen.

  16. Hybrid machine learning technique for forecasting Dhaka stock market timing decisions.

    Science.gov (United States)

    Banik, Shipra; Khodadad Khan, A F M; Anwer, Mohammad

    2014-01-01

    Forecasting stock market has been a difficult job for applied researchers owing to nature of facts which is very noisy and time varying. However, this hypothesis has been featured by several empirical experiential studies and a number of researchers have efficiently applied machine learning techniques to forecast stock market. This paper studied stock prediction for the use of investors. It is always true that investors typically obtain loss because of uncertain investment purposes and unsighted assets. This paper proposes a rough set model, a neural network model, and a hybrid neural network and rough set model to find optimal buy and sell of a share on Dhaka stock exchange. Investigational findings demonstrate that our proposed hybrid model has higher precision than the single rough set model and the neural network model. We believe this paper findings will help stock investors to decide about optimal buy and/or sell time on Dhaka stock exchange.

  17. Step Response Enhancement of Hybrid Stepper Motors Using Soft Computing Techniques

    Directory of Open Access Journals (Sweden)

    Amged S. El-Wakeel

    2014-05-01

    Full Text Available This paper presents the use of different soft computing techniques for step response enhancement of Hybrid Stepper Motors. The basic differential equations of hybrid stepper motor are used to build up a model using MATLAB software package. The implementation of Fuzzy Logic (FL and Proportional-Integral-Derivative (PID controllers are used to improve the motor performance. The numerical simulations by a PC-based controller show that the PID controller tuned by Genetic Algorithm (GA produces better performance than that tuned by Fuzzy controller. They show that, the Fuzzy PID-like controller produces better performance than the other linear Fuzzy controllers. Finally, the comparison between PID controllers tuned by genetic algorithm and the Fuzzy PID-like controller shows that, the Fuzzy PID-like controller produces better performance.

  18. Feasibility of using fluorescence in situ hybridization (FISH) to detect early gene changes in sputum cells from uranium miners

    Energy Technology Data Exchange (ETDEWEB)

    Neft, R.E.; Rogers, J.L.; Belinsky, S.A. [and others

    1995-12-01

    Epidemiological studies have shown that combined exposure to radon progeny and tobacco smoke produce a greater than additive or synergistic increase in lung cancer risk. Lung cancer results from multiple genetic changes over a long period of time. An early change that occurs in lung cancer is trisomy 7 which is found in 50% of non-small cell lung cancer and in the far margins of resected lung tumors. The 80% mortality associated with lung cancer is in part related to the high proportion of patients who present with an advanced, unresectable tumor. Therefore, early detection of patients at risk for tumor development is critical to improve treatment of this disease. Currently, it is difficult to detect lung cancer early while it is still amendable by surgery. Saccomanno, G. has shown that premalignant cytologic changes in sputum cells collected from uranium miners can be detected by a skilled, highly trained cytopathologist. A more objective alternative for identifying premalignant cells in sputum may be to determine whether an early genetic change such as trisomy 7 is present in these cells. Fluorescence in situ hybridization (FISH) can be used to identify cells with trisomy 7. The results of this investigation indicate that FISH may prove to be an accurate, efficient method to test at-risk individuals for genetic alterations in bronchial epithelial cells from sputum.

  19. Comparison of conventional culture method and fluorescent in situ hybridization technique for detection of Listeria spp. in ground beef, turkey, and chicken breast fillets in İzmir, Turkey.

    Science.gov (United States)

    Baysal, Ayse Handan

    2014-12-01

    The occurrence of Listeria species in refrigerated fresh chicken breast fillet, turkey breast fillet, and ground beef was evaluated, comparing the conventional culture method and fluorescent in situ hybridization (FISH). FISH uses hybridization of a nucleic acid sequence target of a microorganism with a specific DNA probe labeled with a fluorochrome and imaging by a fluorescence microscope. First, Listeria was inoculated in chicken breast fillet, turkey breast fillet, or ground beef, and the applicability of the FISH method was evaluated. Second, Listeria was detected in fresh chicken breast fillet, turkey breast fillet, and ground beef by culture and FISH methods. Listeria was isolated from 27 (37.4%) of 216 samples by the standard culture method, whereas FISH detected 25 (24.7%) preenriched samples. Of these isolates, 17 (63%) were L. innocua, 6 (22%) L. welshimeri, and 4 (14.8%) L. seeligeri. Overall, the prevalences of Listeria spp. found with the conventional culture method in chicken breast fillet, turkey breast fillet, and ground beef were 9.7, 6.9, and 20.8%, whereas with the FISH technique these values were 11.1, 6.9, and 16.7%, respectively. The molecular FISH technique appears to be a cheap, sensitive, and time-efficient procedure that could be used for routine detection of Listeria spp. in meat. This study showed that retail raw meats are potentially contaminated with Listeria spp. and are, thus, vehicles for transmitting diseases caused by foodborne pathogens, underlining the need for increased precautions, such as implementation of hazard analysis and critical control points and consumer food safety education.

  20. Vegetated Riprap Installation Techniques for Steambank Protection, Fish and Wildlife Habitat Creation

    Science.gov (United States)

    Raymond, Pierre

    2014-05-01

    Vegetated riprap is a cost effective alternative to conventional riprap erosion protection. Terra Erosion Control has experimented with the vegetation of riprap over the past ten years. As a result we have adapted a technique that can successfully establish vegetation during the installation of riprap structures. This presentation will demonstrate innovative ways of installing vegetated riprap for the protection of access roads on industrial sites and urban infrastructure such as storm water outfalls, bridge approaches and pedestrian pathways within public areas. This vegetation will provide additional bank protection, soften the rock appearance and enhance fish, wildlife and urban habitat along the shoreline. Vegetated riprap incorporates a combination of rock and native vegetation in the form of live cuttings. These are planted in conjunction with the placement of rock used to armour the banks of watercourses. Establishment of native vegetation will improve fish habitat by creating shade, cover and an input of small organic debris to stream banks. In most cases it will negate the need for the regulator (Canadian Department of Fisheries and Oceans) to require habitat alteration compensation. It will also provide added bank protection through the development of root mass. Adding vegetation to riprap provides a softer, more natural appearance to the installed rocks. This presentation will detail the processes involved in the installation of vegetated riprap such as the harvesting and soaking of live material, site preparation of the stream bank, placement of riprap in conjunction with live material and the use of burlap/coir fabric and soil amendments. It will also discuss the innovative method of using wooden boards to protect live cuttings during construction and to direct precipitation and/or irrigation water to the root zone during the establishment phase of the vegetation. These boards will eventually biodegrade within the rock. This approach was applied over

  1. New DAPI and FISH findings on egg maturation processes in related hybridogenetic and parthenogenetic Bacillus hybrids (Insecta, Phasmatodea).

    Science.gov (United States)

    Marescalchi, O; Scali, V

    2001-10-01

    Bacillus stick insects have proved adequate for studying a wide array of reproductive modes: sexual, parthenogenetic, hybridogenetic, androgenetic. Hybridogenetic strains (B. rossius-grandii) were thought to discard the paternal "grandii" haploset during first meiotic division and keep the "rossius" hemiclone, whereas the clonal B. whitei (=rossius/grandii) would maintain its hybrid structure by fusing back two nonsister nuclei-each derived from previously segregated heterospecific complements-by the end of the 2(nd) meiotic division. New investigations on laid eggs and ovariole squashes, either DAPI stained or FISH labeled, revealed that in hybridogens the "grandii" set is excluded from the germ line prior to meiosis and that a DNA extra-synthesis should occur to produce hemiclonal eggs after two cytologically normal meiotic divisions. On the other hand, in B. whitei eggs no genome segregation appears to occur and an intrameiotic DNA extra-synthesis must take place to produce 2n tetrachromatidic oocytes I; these divide twice and give unreduced clonal eggs. The new findings bring hybridogenetic oogenesis of Bacillus to be coincident with that of the known hemiclonal organisms and point to an independent onset of B. whitei from hemiclonal strains. In addition, B. whitei gains a closer resemblance to B. lynceorum owing to the sharing of a cytologically identical egg maturation mechanism, of the same maternal ancestor and of peculiar chromosomal features. It is here suggested that B. lynceorum originated from the incorporation of an "atticus" genome into a B. whitei egg, according to a pathway of repeated hybridization often occurred with other polyploid hybrids.

  2. Hybrid PIV-PTV technique for measuring blood flow in rat mesenteric vessels.

    Science.gov (United States)

    Ha, Hojin; Nam, Kweon-Ho; Lee, Sang Joon

    2012-11-01

    The micro-particle tracking velocimetry (μ-PTV) technique is used to obtain the velocity fields of blood flow in the microvasculature under in vivo conditions because it can provide the blood velocity distribution in microvessels with high spatial resolution. The in vivo μ-PTV technique usually requires a few to tens of seconds to obtain a whole velocity profile across the vessel diameter because of the limited number density of tracer particles under in vivo conditions. Thus, the μ-PTV technique alone is limited in measuring unsteady blood flows that fluctuate irregularly due to the heart beating and muscle movement in surrounding tissues. In this study, a new hybrid PIV-PTV technique was established by combining PTV and particle image velocimetry (PIV) techniques to resolve the drawbacks of the μ-PTV method in measuring blood flow in microvessels under in vivo conditions. Images of red blood cells (RBCs) and fluorescent particles in rat mesenteric vessels were obtained simultaneously. Temporal variations of the centerline blood velocity were monitored using a fast Fourier transform-based cross-correlation PIV method. The fluorescence particle images were analyzed using the μ-PTV technique to extract the spatial distribution of the velocity vectors. Data from the μ-PTV and PIV methods were combined to obtain a better estimate of the velocity profile in actual blood flow. This technique will be useful in investigating hemodynamics in microcirculation by measuring unsteady irregular blood flows more accurately.

  3. A Low Cost Vision Based Hybrid Fiducial Mark Tracking Technique for Mobile Industrial Robots

    Directory of Open Access Journals (Sweden)

    Mohammed Y Aalsalem

    2012-07-01

    Full Text Available The field of robotic vision is developing rapidly. Robots can react intelligently and provide assistance to user activities through sentient computing. Since industrial applications pose complex requirements that cannot be handled by humans, an efficient low cost and robust technique is required for the tracking of mobile industrial robots. The existing sensor based techniques for mobile robot tracking are expensive and complex to deploy, configure and maintain. Also some of them demand dedicated and often expensive hardware. This paper presents a low cost vision based technique called “Hybrid Fiducial Mark Tracking” (HFMT technique for tracking mobile industrial robot. HFMT technique requires off-the-shelf hardware (CCD cameras and printable 2-D circular marks used as fiducials for tracking a mobile industrial robot on a pre-defined path. This proposed technique allows the robot to track on a predefined path by using fiducials for the detection of Right and Left turns on the path and White Strip for tracking the path. The HFMT technique is implemented and tested on an indoor mobile robot at our laboratory. Experimental results from robot navigating in real environments have confirmed that our approach is simple and robust and can be adopted in any hostile industrial environment where humans are unable to work.

  4. Design and Development of Hybrid Multilevel Inverter employing Dual Reference Modulation Technique for Fuel Cell Applications

    Directory of Open Access Journals (Sweden)

    R. Seyezhai

    2011-10-01

    Full Text Available MultiLevel Inverter (MLI has been recognized as an attractive topology for high voltage DC-AC conversion. This paper focuses on a new dual reference modulation technique for a hybrid multilevel inverter employing Silicon carbide (SiC switches for fuel cell applications. The proposed modulation technique employs two reference waveforms and a single inverted sine wave as the carrier waveform. This technique is compared with the conventional dual carrier waveform in terms of output voltage spectral quality and switching losses. An experimental five-level hybrid inverter test rig has been built using SiC switches to implement the proposed algorithm. Gating signals are generated using PIC microcontroller. The performance of the inverter has been analyzed and compared with the result obtained from theory and simulation. Simulation study of Proportional Integral (PI controller for the inverter employing the proposed modulation strategy has been done in MATLAB/SIMULINK. Keywords: Multilevel inverter, SiC , dual reference modulation, switching losses, PI

  5. Hardwood species classification with DWT based hybrid texture feature extraction techniques

    Indian Academy of Sciences (India)

    Arvind R Yadav; R S Anand; M L Dewal; Sangeeta Gupta

    2015-12-01

    In this work, discrete wavelet transform (DWT) based hybrid texture feature extraction techniques have been used to categorize the microscopic images of hardwood species into 75 different classes. Initially, the DWT has been employed to decompose the image up to 7 levels using Daubechies (db3) wavelet as decomposition filter. Further, first-order statistics (FOS) and four variants of local binary pattern (LBP) descriptors are used to acquire distinct features of these images at various levels. The linear support vector machine (SVM), radial basis function (RBF) kernel SVM and random forest classifiers have been employed for classification. The classification accuracy obtained with state-of-the-art and DWT based hybrid texture features using various classifiers are compared. The DWT based FOS-uniform local binary pattern (DWTFOSLBPu2) texture features at the 4th level of image decomposition have produced best classification accuracy of 97.67 ± 0.79% and 98.40 ± 064% for grayscale and RGB images, respectively, using linear SVM classifier. Reduction in feature dataset by minimal redundancy maximal relevance (mRMR) feature selection method is achieved and the best classification accuracy of 99.00 ± 0.79% and 99.20 ± 0.42% have been obtained for DWT based FOS-LBP histogram Fourier features (DWTFOSLBP-HF) technique at the 5th and 6th levels of image decomposition for grayscale and RGB images, respectively, using linear SVM classifier. The DWTFOSLBP-HF features selected with mRMR method has also established superiority amongst the DWT based hybrid texture feature extraction techniques for randomly divided database into different proportions of training and test datasets.

  6. Establishment of a Multi-color Genomic in situ Hybridization Technique to Simultaneously Discriminate the Three Interspecific Hybrid Genomes in Gossypium

    Institute of Scientific and Technical Information of China (English)

    Bing Guan; Kai Wang; Bao-Liang Zhou; Wang-Zhen Guo; Tian-Zhen Zhang

    2008-01-01

    To identify alien chromosomes in recipient progenies and to analyze genome components in polyploidy, a genomic In situ hybridization (GISH) technique that is suitable for cotton was developed using increased stringency conditions. The increased stringency conditions were a combination of the four factors in the following optimized state: 100:1 ratio of blocking DNA to probe, 60% formamide wash solution, 43 =C temperature wash and a 13 min wash. Under these specific conditions using gDNA from Gossypium sturtianurn (C1C1) as a probe, strong hybridization signals were only observed on chromosomes from the C1 genome in somatic cells of the hybrid F1 (G. hirsutum×G. sturtianum) (AtDtC1). Therefore, GISH was able to discriminate parental chromosomes in the hybrid. Further, we developed a multi-color GISH to simultaneously discriminate the three genomes of the above hybrid. The results repeatedly displayed the three genomes, At, Dt, and C1, and each set of chromosomes with a unique color, making them easy to identify. The power of the multi-color GISH was proven by analysis of the hexaploid hybrid F1 (G. hirsutum × G. australe) (AtAtDtDtG2G2). We believe that the powerful multi-color GISH technique could be applied extensively to analyze the genome component in polyploidy and to identify alien chromosomes in the recipient progenies.

  7. Diagnosis of a constitutional five-chromosome rearrangement by fluorescent in situ hybridization (FISH)

    Energy Technology Data Exchange (ETDEWEB)

    Tsien, F.; Shapira, E. [Tulane Univ. School of Medicine, New Orleans, LA (United States); Carvalho, T. [Hospital Sarah Kubitschek, Brasilia (Brazil)] [and others

    1994-09-01

    Complex chromosomal rearrangements are structural rearrangements involving at least three chromosomes and three or more chromosome breakpoints. Such karyotypes are often acquired during cancer multi-step development and in chromosome instability syndromes. However, extremely rare constitutional forms have been reported, most of which are incompatible with life. We present a 2-year-old female with de novo complex rearrangement consisting of five chromosomes and nine breakpoints. Clinical evaluation at two years of age revealed a weight of 5 kg, length of 66 cm, and had circumference of 38 cm, all below the 5th percentile, microcephaly, trigonocephaly, epicanthal folds, inguinal hernia, left clubfoot, hypertonicity, and developmental delay. The neurological examination revealed chorea-acanthocytosis and psychomotor delay. Cultured lymphocytes and fibroblasts revealed a karyotype consisting of five derivative chromosomes. The metaphases were further analyzed by FISH using chromosome-specific libraries and telomeric probes in order to delineate the composition of the rearranged chromosomes; FISH results demonstrated a karyotype of: 46,XX,1pter{r_arrow}1q25::1q42.1{r_arrow}1qter, 2pter{r_arrow}q32.3::1q32.3{r_arrow}2q41::2q37.3{r_arrow}2qter, 7qter{r_arrow}7q21.2::6q22.3{r_arrow}6qter::1q31{r_arrow}1q32.3::6p23{r_arrow}6q22.3, 7pter{r_arrow}7q21.1::6p23{r_arrow}6pter, 2q33{r_arrow}2q37, 1::9p21{r_arrow}9qter. This analysis demonstrates the usefulness of FISH in characterizing complex chromosome rearrangements otherwise difficult to correctly interpret using classical cytogenetics alone.

  8. Direct detection of expanded trinucleotide repeats using PCR and DNA hybridization techniques

    Energy Technology Data Exchange (ETDEWEB)

    Petronis, A.; Tatuch, Y.; Klempan, T.A.; Kennedy, J.L. [Hospital for Sick Children, Toronto (Canada)] [and others

    1996-02-16

    Recently, unstable trinucleotide repeats have been shown to be the etiologic factor in seven neuropsychiatric diseases, and they may play a similar role in other genetic disorders which exhibit genetic anticipation. We have tested one polymerase chain reaction (PCR)-based and two hybridization-based methods for direct detection of unstable DNA expansion in genomic DNA. This technique employs a single primer (asymmetric) PCR using total genomic DNA as a template to efficiently screen for the presence of large trinucleotide repeat expansions. High-stringency Southern blot hybridization with a PCR-generated trinucleotide repeat probe allowed detection of the DNA fragment containing the expansion. Analysis of myotonic dystrophy patients containing different degrees of (CTG){sub n} expansion demonstrated the identification of the site of trinucleotide instability in some affected individuals without any prior information regarding genetic map location. The same probe was used for fluorescent in situ hybridization and several regions of (CTG){sub n}/(CAG){sub n} repeats in the human genome were detected, including the myotonic dystrophy locus on chromosome 19q. Although limited at present to large trinucleotide repeat expansions, these strategies can be applied to directly clone genes involved in disorders caused by large expansions of unstable DNA. 33 refs., 4 figs.

  9. Biocompatibility improvement of titanium implants by coating with hybrid materials synthesized by sol-gel technique.

    Science.gov (United States)

    Catauro, M; Bollino, F; Papale, F

    2014-12-01

    Organic-inorganic hybrid materials based on zirconia and polyethylene glycol (PEG) have been synthesized via sol-gel method in the present study. Those materials, still in the sol phase, have been used to coat a titanium grade 4 (Ti-4) substrate to improve its biological properties. Dip-coating technique has been used to obtain thin films. PEG, a biocompatible polymer, used as the organic phase, has been incorporated with different percentages in an inorganic zirconium-based matrix. Those hybrids have been characterized by Fourier transform infrared spectroscopy (FTIR) to detect interactions between the two phases. The films have been examined using SEM to detect morphological changes with PEG percentages. The potential applications of the hybrid coatings in biomedical field have been evaluated by bioactivity and cytotoxicity tests. The coated titanium was immersed in simulated body fluid (SBF) for 21 days and the hydroxyapatite deposition on its surface was subsequently evaluated, as that feature can be used as an index of bone-bonding capability. SEM equipped with energy dispersive spectrometer (EDS) was used to examine hydroxyapatite formation. NIH 3T3 mouse embryonic fibroblast cells were seeded on specimens to evaluate cells-materials interactions and cell vitality was inspected using WST-8 Assay.

  10. Production of CaCO3/hyperbranched polyglycidol hybrid films using spray-coating technique.

    Science.gov (United States)

    Malinova, Kalina; Gunesch, Manfred; Montero Pancera, Sabrina; Wengeler, Robert; Rieger, Bernhard; Volkmer, Dirk

    2012-05-15

    Biomineralizing organisms employ macromolecules and cellular processing strategies in order to produce highly complex composite materials such as nacre. Bionic approaches translating this knowledge into viable technical production schemes for a large-scale production of biomimetic hybrid materials have met with limited success so far. Investigations presented here thus focus on the production of CaCO(3)/polymer hybrid coatings that can be applied to huge surface areas via reactive spray-coating. Technical requirements for simplicity and cost efficiency include a straightforward one-pot synthesis of low molecular weight hyperbranched polyglycidols (polyethers of 2,3-epoxy-1-propanol) as a simple mimic of biological macromolecules. Polymers functionalized with phosphate monoester, sulfate or carboxylate groups provide a means of controlling CaCO(3) particle density and morphology in the final coatings. We employ reactive spray-coating techniques to generate CaCO(3)/hybrid coatings among which vaterite composites can be prepared in the presence of sulfate-containing hyperbranched polyglycidol. These coatings show high stability and remained unchanged for periods longer than 9 months. By employing carboxylate-based hyperbranched polyglycidol, it is possible to deposit vaterite-calcite composites, whereas phosphate-ester-based hyperbranched polyglycidol leads to calcite composites. Nanoindentation was used to study mechanical properties, showing that coatings thus obtained are slightly harder than pure calcite. Copyright © 2012 Elsevier Inc. All rights reserved.

  11. Empleo de la técnica hibridación in situ fluorescente para visualizar microorganismos Use of fluorescence in situ hybridization technique to visualize microorganisms

    Directory of Open Access Journals (Sweden)

    Raúl Rodríguez Martínez

    2011-12-01

    Full Text Available La hibridación in situ fluorescente (FISH, es una técnica que emplea sondas de oligonucleótidos marcadas con fluorocromos las cuales van dirigidas hacia secuencias específicas del ácido ribonucleico ribosomal (ARNr, lo que permite la identificación rápida y específica de células microbianas ya sea que estén como células individuales o se encuentren agrupadas en su ambiente natural. El conocimiento de la composición y distribución de los microorganismos en los hábitats naturales, proporciona un soporte sólido para comprender la interacción entre las diversas especies que componen el micro hábitat. El objetivo de la revisión es presentar la forma como ha evolucionado la hibridación, el empleo del ARNr como molécula diana, los tipos de marcaje, los marcadores fluorescentes empleados hoy en día, la metodología, así como las mejoras que se le han hecho a la técnica FISH al emplearse en conjunto con otras técnicas en la identificación microbiana. Salud UIS 2011; 43 (3: 307-316Fluorescence in situ hybridization (FISH, is a technique that uses oligonucleotides probes labeled with fluorochromes which are directed to specific sequences of ribosomal ribonucleic acid (rRNA, this allows the rapid and specific identification of microbial cells whether as individual cells or grouped cells in their natural environment. Knowledge of the composition and distribution of microorganisms in natural habitats provides a solid support to understand interaction between different species in the microhabitat. This review shows how hybridization has evolved, the use of rRNA as target molecule, the type of labeling, the labeled uses today in fluorescent and the methodology, as well as the improvements that have been made to the FISH technique when is used in conjunction with other techniques in microbial identification. Salud UIS 2011; 43 (3: 307-316

  12. Hybrid OPC modeling with SEM contour technique for 10nm node process

    Science.gov (United States)

    Hitomi, Keiichiro; Halle, Scott; Miller, Marshal; Graur, Ioana; Saulnier, Nicole; Dunn, Derren; Okai, Nobuhiro; Hotta, Shoji; Yamaguchi, Atsuko; Komuro, Hitoshi; Ishimoto, Toru; Koshihara, Shunsuke; Hojo, Yutaka

    2014-03-01

    Hybrid OPC modeling is investigated using both CDs from 1D and simple 2D structures and contours extracted from complex 2D structures, which are obtained by a Critical Dimension-Scanning Electron Microscope (CD-SEM). Recent studies have addressed some of key issues needed for the implementation of contour extraction, including an edge detection algorithm consistent with conventional CD measurements, contour averaging and contour alignment. Firstly, pattern contours obtained from CD-SEM images were used to complement traditional site driven CD metrology for the calibration of OPC models for both metal and contact layers of 10 nm-node logic device, developed in Albany Nano-Tech. The accuracy of hybrid OPC model was compared with that of conventional OPC model, which was created with only CD data. Accuracy of the model, defined as total error root-mean-square (RMS), was improved by 23% with the use of hybrid OPC modeling for contact layer and 18% for metal layer, respectively. Pattern specific benefit of hybrid modeling was also examined. Resist shrink correction was applied to contours extracted from CD-SEM images in order to improve accuracy of the contours, and shrink corrected contours were used for OPC modeling. The accuracy of OPC model with shrink correction was compared with that without shrink correction, and total error RMS was decreased by 0.2nm (12%) with shrink correction technique. Variation of model accuracy among 8 modeling runs with different model calibration patterns was reduced by applying shrink correction. The shrink correction of contours can improve accuracy and stability of OPC model.

  13. SU-E-T-16: A Hybrid VMAT/IMRT Technique for the Treatment of Nasopharyngeal Carcinoma

    Energy Technology Data Exchange (ETDEWEB)

    Zhao, N; Yang, R; Wang, J [Peking University Third Hospital, Beijing, Beijing (China)

    2014-06-01

    Purpose: To investigate a Hybrid VMAT/IMRT technique which combines volumetric modulated arc therapy (VMAT) and intensity modulated radiation therapy (IMRT) for nasopharyngeal carcinoma (NPC). Methods: 2 full arcs VMAT, 9-field IMRT and Hybrid VMAT/IMRT plans were created for 10 patients with NPC. The Hybrid VMAT/IMRT technique consisted of 1 full VMAT arc and 7 IMRT fields. The dose distribution of planning target volume (PTV) and organs at risk (OARs) for Hybrid VMAT/IMRT was compared with IMRT and VMAT. The monitor units (MUs) were also evaluated. Results: The Hybrid VMAT/IMRT technique significantly improved target dose homogeneity compared with IMRT and VMAT for PTV70 and PTV54. For PTV70 and PTV60, the Hybrid VMAT/IMRT technique significantly improved target dose conformity compared with IMRT (0.62 vs 0.47; p<0.05 and 0.64 vs 0.58; p<0.05, respectively) and VMAT (0.62 vs 0.43; p<0.05 and 0.64 vs 0.6; p<0.05, respectively). The near maximum dose (D2%) of temporomandibular joint (TMJ), temporal lobe and mandible for Hybrid plans were 5.5%, 7.9% and 5.2% lower than IMRT plans (p<0.05). The mean dose of TMJ, temporal lobe, mandible and unspecified tissue for Hybrid plans were 12.8%, 11.4%, 4.2% and 4.1% lower than IMRT plans (p<0.05). The mean dose of right parotid, mandible and unspecified tissue for Hybrid plans were 3.3%, 2.4% and 3.1% lower than VMAT plans (p<0.05). The mean MUs needed for IMRT, VMAT and Hybrid plans were 2256, 507 and 1394, respectively. Conclusion: Hybrid VMAT/IMRT technique significantly improved the target dose homogeneity and conformity compared with IMRT and VMAT and reduced the dose of OARs and unspecified tissue compared with IMRT with fewer MUs. Compared with VMAT, Hybrid VMAT/IMRT technique can better protect parotid gland, mandible and unspecified tissue. Ruijie Yang was funded by the grant project: National Natural Science Foundation of China (No. 81071237). Other authors have no competing interest for this work.

  14. Evaluating machine-learning techniques for recruitment forecasting of seven North East Atlantic fish species

    KAUST Repository

    Fernandes, José Antonio

    2015-01-01

    The effect of different factors (spawning biomass, environmental conditions) on recruitment is a subject of great importance in the management of fisheries, recovery plans and scenario exploration. In this study, recently proposed supervised classification techniques, tested by the machine-learning community, are applied to forecast the recruitment of seven fish species of North East Atlantic (anchovy, sardine, mackerel, horse mackerel, hake, blue whiting and albacore), using spawning, environmental and climatic data. In addition, the use of the probabilistic flexible naive Bayes classifier (FNBC) is proposed as modelling approach in order to reduce uncertainty for fisheries management purposes. Those improvements aim is to improve probability estimations of each possible outcome (low, medium and high recruitment) based in kernel density estimation, which is crucial for informed management decision making with high uncertainty. Finally, a comparison between goodness-of-fit and generalization power is provided, in order to assess the reliability of the final forecasting models. It is found that in most cases the proposed methodology provides useful information for management whereas the case of horse mackerel is an example of the limitations of the approach. The proposed improvements allow for a better probabilistic estimation of the different scenarios, i.e. to reduce the uncertainty in the provided forecasts.

  15. Apical transportation of manual NiTi instruments and a hybrid technique in severely curved simulated canals

    OpenAIRE

    Aurenaila Nascimento Gonçalves; Matheus Franco da Frota; Emilio Carlos Sponchiado Júnior; Fredson Marcio Acris de Carvalho; Lucas da Fonseca Roberti Garcia; André Augusto Franco Marques

    2015-01-01

    Aim: To evaluate the apical transportation induced by two instrumentation techniques in severely curved simulated canals. Materials and Methods: Forty simulated canals were divided into two groups (n = 20), according to the following instrumentation techniques: ProTaper Universal Manual System and a hybrid technique. The simulated canals in the ProTaper group were prepared following the technique recommended by the manufacturer: SX files in the cervical third of the root canal and S1, S2,...

  16. Enhancement in Seismic Imaging using Diffraction Studies and Hybrid Traveltime Technique for PSDM

    Science.gov (United States)

    Bashir, Y.; Ghosh, D. P.; Moussavi Alashloo, S. Y.; Sum, C. W.

    2016-07-01

    The accurate migration of seismic data is conditional on the parameters which are nominated. The effective velocity used in residual processing for migration is small compared to the original migration velocity. Considering traveltime computation is a significant part of seismic imaging algorithms. Conventional implementation of Kirchhoff migration is essential for precomputing a traveltime table from the categories involving traditional ray-tracing methods and finite difference eikonal solvers. In this paper, we examine the accuracy using, the eikonal solver and paraxial ray tracing traveltime computation in pre-stack Kirchhoff depth migration. This hybrid traveltime technique can be applied to a variety of problems related to faults, fractures, and complex region. To evaluate the relevance of this identical traveltime technique, we applied on a Marmousi data set.

  17. REVIEW OF HEART DISEASE PREDICTION SYSTEM USING DATA MINING AND HYBRID INTELLIGENT TECHNIQUES

    Directory of Open Access Journals (Sweden)

    R. Chitra

    2013-07-01

    Full Text Available The Healthcare industry generally clinical diagnosis is done mostly by doctor’s expertise and experience. Computer Aided Decision Support System plays a major role in medical field. With the growing research on heart disease predicting system, it has become important to categories the research outcomes and provides readers with an overview of the existing heart disease prediction techniques in each category. Neural Networks are one of many data mining analytical tools that can be utilized to make predictions for medical data. From the study it is observed that Hybrid Intelligent Algorithm improves the accuracy of the heart disease prediction system. The commonly used techniques for Heart Disease Prediction and their complexities are summarized in this paper.

  18. Local tetrahedron modeling of microelectronics using the finite-volume hybrid-grid technique

    Energy Technology Data Exchange (ETDEWEB)

    Riley, D.J.; Turner, C.D.

    1995-12-01

    The finite-volume hybrid-grid (FVHG) technique uses both structured and unstructured grid regions in obtaining a solution to the time-domain Maxwell`s equations. The method is based on explicit time differencing and utilizes rectilinear finite-difference time-domain (FDTD) and nonorthogonal finite-volume time-domain (FVTD). The technique directly couples structured FDTD grids with unstructured FVTD grids without the need for spatial interpolation across grid interfaces. In this paper, the FVHG method is applied to simple planar microelectronic devices. Local tetrahedron grids are used to model portions of the device under study, with the remainder of the problem space being modeled with cubical hexahedral cells. The accuracy of propagating microstrip-guided waves from a low-density hexahedron region through a high-density tetrahedron grid is investigated.

  19. Enhancement of Twins Fetal ECG Signal Extraction Based on Hybrid Blind Extraction Techniques

    Directory of Open Access Journals (Sweden)

    Ahmed Kareem Abdullah

    2017-07-01

    Full Text Available ECG machines are noninvasive system used to measure the heartbeat signal. It’s very important to monitor the fetus ECG signals during pregnancy to check the heat activity and to detect any problem early before born, therefore the monitoring of ECG signals have clinical significance and importance. For multi-fetal pregnancy case the classical filtering algorithms are not sufficient to separate the ECG signals between mother and fetal. In this paper the mixture consists of mixing from three ECG signals, the first signal is the mother ECG (M-ECG signal, second signal the Fetal-1 ECG (F1-ECG, and third signal is the Fetal-2 ECG (F2-ECG, these signals are extracted based on modified blind source extraction (BSE techniques. The proposed work based on hybridization between two BSE techniques to ensure that the extracted signals separated well. The results demonstrate that the proposed work very efficiently to extract the useful ECG signals

  20. Estimation of radon concentrations in coal mines using a hybrid technique calibration curve.

    Science.gov (United States)

    Jamil, K; Ali, S

    2001-01-01

    The results of epidemiological studies in various countries show that radon and its progeny cause carcinogenic effects on mine workers. Therefore, it becomes of paramount importance to monitor radon concentrations and consequently determine the radon dose rates in coal mines for the protection of coal miners. A new calibration curve was obtained for radon concentration estimation using hybrid techniques. A calibration curve was generated using 226Ra activity concentration measured by a HPGe detector-based gamma-ray spectrometer versus alpha-track-density rate due to radon and its progeny on CR-39 track detector. Using the slope of the experimentally determined curve in the units of Becqueral per kilogram (Bq kg-1) per unit alpha-track-density per hour (cm-2 h-1), radon concentrations (Bq m-3) were estimated using coal samples from various coal mines in two provinces of Pakistan, Punjab and Balochistan. Consequently, radon dose rates were computed in the simulated environment of the coal mines. Results of these computations may be considered with a caveat that the method developed in this paper provides only a screening method to indicate the radon dose in coal mines. It has been shown that the actual measurements of radon concentrations in the coal mines are in agreement with the estimated radon concentrations using the hybrid-technique calibration curve.

  1. Hybrid Model Testing Technique for Deep-Sea Platforms Based on Equivalent Water Depth Truncation

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    In this paper, an inner turret moored FPSO which works in the water of 320 m depth, is selected to study the so-called "passively-truncated + numerical-simulation" type of hybrid model testing technique while the truncated water depth is 160 m and the model scale λ=80. During the investigation, the optimization design of the equivalent-depth truncated system is performed by using the similarity of the static characteristics between the truncated system and the full depth one as the objective function. According to the truncated system, the corresponding physical test model is made. By adopting the coupling time domain simulation method, the truncated system model test is numerically reconstructed to carefully verify the computer simulation software and to adjust the corresponding hydrodynamic parameters. Based on the above work, the numerical extrapolation to the full depth system is performed by using the verified computer software and the adjusted hydrodynamic parameters. The full depth system model test is then performed in the basin and the results are compared with those from the numerical extrapolation. At last, the implementation procedure and the key technique of the hybrid model testing of the deep-sea platforms are summarized and printed. Through the above investigations, some beneficial conclusions are presented.

  2. Novel Hybrid Scheduling Technique for Sensor Nodes with Mixed Criticality Tasks

    Directory of Open Access Journals (Sweden)

    Mihai-Victor Micea

    2017-06-01

    Full Text Available Sensor networks become increasingly a key technology for complex control applications. Their potential use in safety- and time-critical domains has raised the need for task scheduling mechanisms specially adapted to sensor node specific requirements, often materialized in predictable jitter-less execution of tasks characterized by different criticality levels. This paper offers an efficient scheduling solution, named Hybrid Hard Real-Time Scheduling (H2RTS, which combines a static, clock driven method with a dynamic, event driven scheduling technique, in order to provide high execution predictability, while keeping a high node Central Processing Unit (CPU utilization factor. From the detailed, integrated schedulability analysis of the H2RTS, a set of sufficiency tests are introduced and demonstrated based on the processor demand and linear upper bound metrics. The performance and correct behavior of the proposed hybrid scheduling technique have been extensively evaluated and validated both on a simulator and on a sensor mote equipped with ARM7 microcontroller.

  3. Skill Assessment of An Hybrid Technique To Estimate Quantitative Precipitation Forecast For Galicia (nw Spain)

    Science.gov (United States)

    Lage, A.; Taboada, J. J.

    Precipitation is the most obvious of the weather elements in its effects on normal life. Numerical weather prediction (NWP) is generally used to produce quantitative precip- itation forecast (QPF) beyond the 1-3 h time frame. These models often fail to predict small-scale variations of rain because of spin-up problems and their coarse spatial and temporal resolution (Antolik, 2000). Moreover, there are some uncertainties about the behaviour of the NWP models in extreme situations (de Bruijn and Brandsma, 2000). Hybrid techniques, combining the benefits of NWP and statistical approaches in a flexible way, are very useful to achieve a good QPF. In this work, a new technique of QPF for Galicia (NW of Spain) is presented. This region has a percentage of rainy days per year greater than 50% with quantities that may cause floods, with human and economical damages. The technique is composed of a NWP model (ARPS) and a statistical downscaling process based on an automated classification scheme of at- mospheric circulation patterns for the Iberian Peninsula (J. Ribalaygua and R. Boren, 1995). Results show that QPF for Galicia is improved using this hybrid technique. [1] Antolik, M.S. 2000 "An Overview of the National Weather Service's centralized statistical quantitative precipitation forecasts". Journal of Hydrology, 239, pp:306- 337. [2] de Bruijn, E.I.F and T. Brandsma "Rainfall prediction for a flooding event in Ireland caused by the remnants of Hurricane Charley". Journal of Hydrology, 239, pp:148-161. [3] Ribalaygua, J. and Boren R. "Clasificación de patrones espaciales de precipitación diaria sobre la España Peninsular". Informes N 3 y 4 del Servicio de Análisis e Investigación del Clima. Instituto Nacional de Meteorología. Madrid. 53 pp.

  4. Fuel consumption analysis of diesel-electric hybrid system of fishing boat%渔船柴电混合动力系统油耗分析

    Institute of Scientific and Technical Information of China (English)

    曹建军; 李胜勇

    2016-01-01

    innovative techniques such as multi-machine paddle split system to allow the system greater intelligent deployment role, the hybrid can be further extended to trawlers, purse seiners and other fishing vessels.

  5. Novel Acoustic Techniques for Assessing Fish Schooling in the Context of an Operational Ocean Observatory

    Science.gov (United States)

    2008-09-30

    and onshore waters. A unique acoustic scattering source was identified during the experiment as dense, monotypic aggregations of a pelagic gastropod ...can often reveal hidden coherent structure. IMPACT/ APPLICATIONS The distribution of fish and the variability in their distribution has...direct and waveguide scattering for application to fish. REFERENCES Pitcher, T. J., and J. K. Parrish. 1993. Function of shoaling behavior in teleosts

  6. Protection of fish oil from oxidation by microencapsulation using freeze-drying techniques

    DEFF Research Database (Denmark)

    Heinzelmann, K.; Franke, K.; Jensen, Benny

    2000-01-01

    (N-3)-Polyunsaturated fatty acids (PUFAs) reduce the risk of coronary heart disease. Cold sea water plankton and plankton- consuming fish are known sources of (n-3)-PUFAs. Enriching normal food components with fish oil is a tool for increasing the intake of (n-3)-PUFAs. Due to the high sensitivity...

  7. A hybrid MAS/MoM technique for 2D impedance scatterers illuminated by closely positioned sources

    DEFF Research Database (Denmark)

    Larsen, Niels Vesterdal; Breinbjerg, Olav

    2005-01-01

    A hybrid technique for 2D scattering problems with impedance structures and closely positioned illuminating sources is presented. This technique combines the method of auxiliary sources (MAS) with a localized method of moments (MoM) region near the source. Significant improvements over standard MAS...

  8. Using ordination and clustering techniques to assess multi-metric fish health response following a coal fly ash spill

    Energy Technology Data Exchange (ETDEWEB)

    Bevelhimer, Mark S. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Adams, Marshall [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Fortner, Allison M. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Greeley, Jr, Mark Stephen [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Brandt, Craig C. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

    2014-01-01

    The effect of coal ash exposure on fish health in freshwater communities is largely unknown. Given the large number of possible pathways of effects (e.g., toxicological effect of exposure to multiple metals, physical effects from ash exposure, and food web effects), measurement of only a few health metrics is not likely to give a complete picture. The authors measured a suite of 20 health metrics from 1100+ fish collected from 5 sites (3 affected and 2 reference) near a coal ash spill in east Tennessee over a 4.5-yr period. The metrics represented a wide range of physiological and energetic responses and were evaluated simultaneously using 2 multivariate techniques. Results from both hierarchical clustering and canonical discriminant analyses suggested that for most speciesXseason combinations, the suite of fish health indicators varied more among years than between spill and reference sites within a year. In a few cases, spill sites from early years in the investigation stood alone or clustered together separate from reference sites and later year spill sites. Outlier groups of fish with relatively unique health profiles were most often from spill sites, suggesting that some response to the ash exposure may have occurred. Results from the 2 multivariate methods suggest that any change in the health status of fish at the spill sites was small and appears to have diminished since the first 2 to 3 yr after the spill.

  9. WRHT: A Hybrid Technique for Detection of Wormhole Attack in Wireless Sensor Networks

    Directory of Open Access Journals (Sweden)

    Rupinder Singh

    2016-01-01

    Full Text Available Wormhole attack is a challenging security threat to wireless sensor networks which results in disrupting most of the routing protocols as this attack can be triggered in different modes. In this paper, WRHT, a wormhole resistant hybrid technique, is proposed, which can detect the presence of wormhole attack in a more optimistic manner than earlier techniques. WRHT is based on the concept of watchdog and Delphi schemes and ensures that the wormhole will not be left untreated in the sensor network. WRHT makes use of the dual wormhole detection mechanism of calculating probability factor time delay probability and packet loss probability of the established path in order to find the value of wormhole presence probability. The nodes in the path are given different ranking and subsequently colors according to their behavior. The most striking feature of WRHT consists of its capacity to defend against almost all categories of wormhole attacks without depending on any required additional hardware such as global positioning system, timing information or synchronized clocks, and traditional cryptographic schemes demanding high computational needs. The experimental results clearly indicate that the proposed technique has significant improvement over the existing wormhole attack detection techniques.

  10. Hybrid Clustering-GWO-NARX neural network technique in predicting stock price

    Science.gov (United States)

    Das, Debashish; Safa Sadiq, Ali; Mirjalili, Seyedali; Noraziah, A.

    2017-09-01

    Prediction of stock price is one of the most challenging tasks due to nonlinear nature of the stock data. Though numerous attempts have been made to predict the stock price by applying various techniques, yet the predicted price is not always accurate and even the error rate is high to some extent. Consequently, this paper endeavours to determine an efficient stock prediction strategy by implementing a combinatorial method of Grey Wolf Optimizer (GWO), Clustering and Non Linear Autoregressive Exogenous (NARX) Technique. The study uses stock data from prominent stock market i.e. New York Stock Exchange (NYSE), NASDAQ and emerging stock market i.e. Malaysian Stock Market (Bursa Malaysia), Dhaka Stock Exchange (DSE). It applies K-means clustering algorithm to determine the most promising cluster, then MGWO is used to determine the classification rate and finally the stock price is predicted by applying NARX neural network algorithm. The prediction performance gained through experimentation is compared and assessed to guide the investors in making investment decision. The result through this technique is indeed promising as it has shown almost precise prediction and improved error rate. We have applied the hybrid Clustering-GWO-NARX neural network technique in predicting stock price. We intend to work with the effect of various factors in stock price movement and selection of parameters. We will further investigate the influence of company news either positive or negative in stock price movement. We would be also interested to predict the Stock indices.

  11. Improved Framework for Breast Cancer Detection using Hybrid Feature Extraction Technique and FFNN

    Directory of Open Access Journals (Sweden)

    Ibrahim Mohamed Jaber Alamin

    2016-10-01

    Full Text Available Breast Cancer early detection using terminologies of image processing is suffered from the less accuracy performance in different automated medical tools. To improve the accuracy, still there are many research studies going on different phases such as segmentation, feature extraction, detection, and classification. The proposed framework is consisting of four main steps such as image preprocessing, image segmentation, feature extraction and finally classification. This paper presenting the hybrid and automated image processing based framework for breast cancer detection. For image preprocessing, both Laplacian and average filtering approach is used for smoothing and noise reduction if any. These operations are performed on 256 x 256 sized gray scale image. Output of preprocessing phase is used at efficient segmentation phase. Algorithm is separately designed for preprocessing step with goal of improving the accuracy. Segmentation method contributed for segmentation is nothing but the improved version of region growing technique. Thus breast image segmentation is done by using proposed modified region growing technique. The modified region growing technique overcoming the limitations of orientation as well as intensity. The next step we proposed is feature extraction, for this framework we have proposed to use combination of different types of features such as texture features, gradient features, 2D-DWT features with higher order statistics (HOS. Such hybrid feature set helps to improve the detection accuracy. For last phase, we proposed to use efficient feed forward neural network (FFNN. The comparative study between existing 2D-DWT feature extraction and proposed HOS-2D-DWT based feature extraction methods is proposed.

  12. Potentials of Optical Damage Assessment Techniques in Automotive Crash-Concepts composed of FRP-Steel Hybrid Material Systems

    Science.gov (United States)

    Dlugosch, M.; Spiegelhalter, B.; Soot, T.; Lukaszewicz, D.; Fritsch, J.; Hiermaier, S.

    2017-05-01

    With car manufacturers simultaneously facing increasing passive safety and efficiency requirements, FRP-metal hybrid material systems are one way to design lightweight and crashworthy vehicle structures. Generic automotive hybrid structural concepts have been tested under crash loading conditions. In order to assess the state of overall damage and structural integrity, and primarily to validate simulation data, several NDT techniques have been assessed regarding their potential to detect common damage mechanisms in such hybrid systems. Significant potentials were found particularly in combining 3D-topography laser scanning and X-Ray imaging results. Ultrasonic testing proved to be limited by the signal coupling quality on damaged or curved surfaces.

  13. A COMPARISION OF VARIOUS EDGE DETECTION TECHNIQUES IN MOTION PICTURE FOR IDENTIFYING A SHARK FISH

    Directory of Open Access Journals (Sweden)

    Shrivakshan Gopal Thiruvangadan

    2013-01-01

    Full Text Available The significant feature of detecting the motion image objects in this study it try identify the shark fish videos by removing the Background of the image. The main method involved in the detecting from the background is the foreground detection of the image. There are many techniques which usually ignore the fact that the background images consist of different image objects whose conditions may mostly change occur. In this study, a motion picture identification procedure is proposed for real time motion video frames by comparing the three key classes of methods for motion detection primarily the Background Removal (Subtraction followed by the Temporal distinguishing (differencing and Optical Flow method. Structured hierarchical background procedure is proposed based on segmenting background images objects. It mainly divided the background images divided into several parts (regions by the Support Vector Machine (SVM followed by a structured hierarchical model is built with the region procedure and pixel model procedure. In the region model method, the image object is extracted from the histogram of specific parts which is same to the kind of a Gaussian-combination model. In the pixel model procedure, it is been demonstrated by histograms, picture, which shows gradients sample of pixels in each parts based on the concurrent occurrence of object variations. In this study, it suggests Silhouette detection procedure and it is used. The experimental result are counter validated with a video database to illustrate its efficiencies, which is involved, from static to dynamic scenes by analyzing it with some distinguished motion detection methods chiefly Temporal differencing method followed by Optical Flow method and based on the outputs a motion detection procedure for real time video frames can be created which is cost effective, it shows good rate of accuracy, which is less rate of reliability in simple, less of complexity and well adapted to several

  14. Locked Nucleic Acid Flow Cytometry-fluorescence in situ Hybridization (LNA flow-FISH): A Method for Bacterial Small RNA Detection

    Science.gov (United States)

    2012-01-10

    Friedrich, U. & Lenke, J. Improved Enumeration of Lactic Acid Bacteria in Mesophilic Dairy Starter Cultures by Using Multiplex Quantitative Real...messenger RNA using locked nucleic acid probes. Anal. Biochem. 390, 109-114 (2009). 13. Waters, L. & Storz, G. Regulatory RNAs in bacteria . Cell. 136, 615...Video Article Locked Nucleic Acid Flow Cytometry-fluorescence in situ Hybridization (LNA flow-FISH): a Method for Bacterial Small RNA Detection Kelly

  15. Phylogeny-function analysis of (meta)genomic libraries: screening for expression of ribosomal RNA genes by large-insert library fluorescent in situ hybridization (LIL-FISH).

    Science.gov (United States)

    Leveau, Johan H J; Gerards, Saskia; de Boer, Wietse; van Veen, Johannes A

    2004-09-01

    We assessed the utility of fluorescent in situ hybridization (FISH) in the screening of clone libraries of (meta)genomic or environmental DNA for the presence and expression of bacterial ribosomal RNA (rRNA) genes. To establish proof-of-principle, we constructed a fosmid-based library in Escherichia coli of large-sized genomic DNA fragments of the mycophagous soil bacterium Collimonas fungivorans, and hybridized 768 library clones with the Collimonas-specific fluorescent probe CTE998-1015. Critical to the success of this approach (which we refer to as large-insert library FISH or LIL-FISH) was the ability to induce fosmid copy number, the exponential growth status of library clones in the FISH assay and the use of a simple pooling strategy to reduce the number of hybridizations. Twelve out of 768 E. coli clones were suspected to harbour and express Collimonas 16S rRNA genes based on their hybridization to CTE998-1015. This was confirmed by the finding that all 12 clones were also identified in an independent polymerase chain reaction-based screening of the same 768 clones using a primer set for the specific detection of Collimonas 16S ribosomal DNA (rDNA). Fosmids isolated from these clones were grouped by restriction analysis into two distinct contigs, confirming that C. fungivorans harbours at least two 16S rRNA genes. For one contig, representing 1-2% of the genome, the nucleotide sequence was determined, providing us with a narrow but informative view of Collimonas genome structure and content.

  16. Three sympatric karyomorphs in the fish Astyanax fasciatus (Teleostei, Characidae) do not seem to hybridize in natural populations

    Science.gov (United States)

    Ferreira-Neto, Maressa; Artoni, Roberto Ferreira; Vicari, Marcelo Ricardo; Moreira-Filho, Orlando; Camacho, Juan Pedro Martínez; Bakkali, Mohammed; de Oliveira, Claudio; Foresti, Fausto

    2012-01-01

    Abstract Ninety individuals of the characid fish Astyanax fasciatus (Cuvier, 1819) were collected at Água da Madalena stream (Botucatu, São Paulo, Brazil) and analyzed for diploid chromosome number 2n and karyotype composition as well as for the chromosomal location of the 5S and 18S ribosomal DNA (rDNA). Whereas no chromosome differences were associated with sex, three different karyomorphs with diploid chromosome numbers 2n=46, 2n=48 and 2n=50 were found. No intermediate 2n numbers were discovered. The 2n=50 karyomorph showed some differences in 18S rDNA location compared to the two other karyomorphs. Finally, all specimens with the 2n=46 karyomorph showed the presence of a partly heterochromatic macro supernumerary chromosome, which was absent in all individuals with the two other karyomorphs. All these results suggest that indviduals of the three different karyomorphs are not likely to hybridize in the examined populations. Our findings strongly suggest the presence of three separate species (sensu biological species concept) easily diagnosed on the basis of differences in the diploid chromosome numbers and other chromosomal markers. PMID:24260650

  17. Three sympatric karyomorphs in the fish Astyanax fasciatus (Teleostei, Characidae do not seem to hybridize in natural populations

    Directory of Open Access Journals (Sweden)

    Maressa Ferreira-Neto

    2012-01-01

    Full Text Available Ninety individuals of the characid fish Astyanax fasciatus (Cuvier, 1819 were collected at Água da Madalena stream (Botucatu, São Paulo, Brazil and analyzed for diploid chromosome number 2n and karyotype composition as well as for the chromosomal location of the 5S and 18S ribosomal DNA (rDNA. Whereas no chromosome differences were associated with sex, three different karyomorphs with diploid chromosome numbers 2n=46, 2n=48 and 2n=50 were found. No intermediate 2n numbers were discovered. The 2n=50 karyomorph showed some differences in 18S rDNA location compared to the two other karyomorphs. Finally, all specimens with the 2n=46 karyomorph showed the presence of a partly heterochromatic macro supernumerary chromosome, which was absent in all individuals with the two other karyomorphs. All these results suggest that indviduals of the three different karyomorphs are not likely to hybridize in the examined populations. Our findings strongly suggest the presence of three separate species (sensu biological species concept easily diagnosed on the basis of differences in the diploid chromosome numbers and other chromosomal markers.

  18. Muskrat population estimates for Fish Springs NWR, Utah : An assessment of techniques

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — Completion report for a study of muskrat population dynamics and vegetation utilization, being led by Utah State University for a doctorate dissertation. The study...

  19. Hydrophobicity control by a supercritical drying technique in a sol–gel process with hybrid materials

    Energy Technology Data Exchange (ETDEWEB)

    Ren, Hongbo; Qiao, Zemin; Liu, Xiao; Cui, Xudong, E-mail: xudcui@gmail.com

    2015-10-15

    Highlights: • Sol–gel route is combined with polymerization without using modifier. • Supercritical drying control is the key to obtain super-hydrophobic surfaces. • The whole fabrication is technologically controllable and with low costs. • The production rate is higher than 90%. • The method provides a cost-effective way for industry applications. - Abstract: We successfully synthesized one type of cheap super-hydrophobic hybrid porous materials in a sol–gel process. In this route, hydrophilic polymers and TEOS-base sol are used as precursors, the ultraviolet ray-initiated polymerization and supercritical fluid drying techniques are combined together to fulfill this task. All fabricated samples exhibit lotus-leaf-like surface structures with super-hydrophobicity. The underlying mechanisms are carefully investigated using a field-emission scanning electron microscopy (FESEM) and an X-ray photoelectron spectroscopy (XPS). We found that a well-controlled drying process is crucial to the formation of such super-hydrophobic surfaces. As high as 90% production rate is obtained in our route and thus, it might provide a cost-effective way to produce super-hydrophobic hybrid materials for industry applications.

  20. Adaptation of Hybrid FSO/RF Communication System Using Puncturing Technique

    Directory of Open Access Journals (Sweden)

    M. N. Khan

    2016-12-01

    Full Text Available Spectrum of radio frequency (RF communications is limited and expensive to install new applications. Free space optical (FSO communication is a viable technology which offers enormous bandwidth, license free installation, inexpensive deployment and error prone links. The FSO links degrade significantly due to the varying atmospheric and weather conditions (fog, cloud, snow, haze and combination of these. We propose a hybrid FSO/RF communication system which adapts the varying nature of atmosphere and weather. For the adaption of varying atmosphere and weather scenarios, we develop a novel optimization algorithm. The proposed algorithm is based on the well-known puncturing technique. We provide an extrinsic information transfer (EXIT chart for the binary and quaternary mapping scheme for the proposed communication system. We simulate the proposed algorithm for the hybrid communication system and analyze the system performance. The proposed algorithm is computationally less expensive and provide better performance gains over varying atmosphere and weather conditions. The algorithm is suitable for fast speed applications.

  1. Identification of seagrasses in the gut of a marine herbivorous fish using DNA barcoding and visual inspection techniques.

    Science.gov (United States)

    Chelsky Budarf, A; Burfeind, D D; Loh, W K W; Tibbetts, I R

    2011-07-01

    Traditional visual diet analysis techniques were compared with DNA barcoding in juvenile herbivorous rabbitfish Siganus fuscescens collected in Moreton Bay, Australia, where at least six species of seagrass occur. The intergenic spacer trnH-psbA, suggested as the optimal gene for barcoding angiosperms, was used for the first time to identify the seagrass in fish guts. Four seagrass species and one alga were identified visually from gut contents; however, there was considerable uncertainty in visual identification with 38 of 40 fish having unidentifiable plant fragments in their gut. PCR and single-strand conformational polymorphism (SSCP) were able to discriminate three seagrass families from visually cryptic gut contents. While effective in identifying cryptic gut content to family level, this novel method is likely to be most efficient when paired with visual identification techniques.

  2. FishPopTrace: a new genetic technique for fisheries monitoring and the identification of IUU

    DEFF Research Database (Denmark)

    Helyar, Sarah; Limborg, Morten; Bekkevold, Dorte

    2012-01-01

    The importance of marine organisms for both economic and ecological reasons is enormous; and knowledge of population structure and connectivity is crucial for the sustainable utilization and conservation of exploited fish stocks. However, in most cases our understanding of these spatial patterns...... nucleotide polymorphisms (SNPs) has the potential to demonstrate previously undetected spatial and temporal population structuring and signatures of adaptive variation. In addition, SNPs are uniquely applicable for the identification and monitoring of wild fish populations and the traceability...

  3. [Interphase cytogenetics of the breast tumors with fluorescence in situ hybridization (FISH) on cytologic preparation--its practice and clinical applications].

    Science.gov (United States)

    Shibuya, M; Osamura, R Y

    1996-05-01

    Fluorescence in situ hybridization (FISH) study with the chromosome specific probes is performed in the interphase nuclei of the routinely processed cytologic preparation of the breast tumors. Numerical aberrations on the chromosomes 1, 3, 11 or 17 were detected in more than 80% of the malignant tumors, but not in the benign tumors. Marked heterogeneity of the polysomies is noted in the malignant tumor cells. A few malignant cases revealed monosomy of chromosome 17. No apparent correlation between the numerical abnormalities and the histological features in malignant tumors is identified. These results suggest that the interphase cytogenetics with FISH for the breast tumors may be useful for differential diagnosis of malignancy. The practice and the clinical applications of the FISH study are discussed.

  4. A millisecond micro-RNA separation technique by a hybrid structure of nanopillars and nanoslits.

    Science.gov (United States)

    Wu, Qiong; Kaji, Noritada; Yasui, Takao; Rahong, Sakon; Yanagida, Takeshi; Kanai, Masaki; Nagashima, Kazuki; Tokeshi, Manabu; Kawai, Tomoji; Baba, Yoshinobu

    2017-03-08

    A millisecond micro-RNA separation of a mixture of total RNA and genomic DNA, extracted from cultured HeLa cells, was successfully achieved using a hybrid structure of nanopillars and nanoslits contained inside a microchannel. The nanopillars, 250-nm in diameter and 100-nm in height, were fabricated with a 750-nm space inside the nanoslits, which were 100-nm in height and 25-μm in width; the nanopillars were then applied as a new sieve matrix. This ultra-fast technique for the separation of miRNA can be an effective pretreatment for semiconductor nanopore DNA sequencing, which has an optimum reading speed of 1 base/ms to obtain effective signal-to-noise ratio and discriminate each base by ion or tunneling current during the passage of nucleic acids.

  5. Modified Genetic Algorithm for DNA Sequence Assembly by Shotgun and Hybridization Sequencing Techniques

    Directory of Open Access Journals (Sweden)

    Prof.Narayan Kumar Sahu

    2012-09-01

    Full Text Available Since the advent of rapid DNA sequencing methods in 1976, scientists have had the problem of inferring DNA sequences from sequenced fragments. Shotgun sequencing is a well-established biological and computational method used in practice. Many conventional algorithms for shotgun sequencing are based on the notion of pair wise fragment overlap. While shotgun sequencing infers a DNA sequence given the sequences of overlapping fragments, a recent and complementary method, called sequencing by hybridization (SBH, infers a DNA sequence given the set of oligomers that represents all sub words of some fixed length, k. In this paper, we propose a new computer algorithm for DNA sequence assembly that combines in a novel way the techniques of both shotgun and SBH methods. Based on our preliminary investigations, the algorithm promises- to be very fast and practical for DNA sequence assembly [1].

  6. Dual Fixation of Calcaneal Tuberosity Avulsion with Concomitant Achilles Tendon Rupture: A Novel Hybrid Technique

    Directory of Open Access Journals (Sweden)

    Gautham Prabhakar

    2017-01-01

    Full Text Available Fracture of the calcaneal tuberosity with a concomitant Achilles tendon rupture presents a difficult challenge for the treating surgeon. The ultimate goal of treatment is to restore function of both the gastrocnemius-soleus complex and the Achilles tendon. This particular subset of fractures occurs often in diabetics and elderly patients with osteoporosis making fixation of the displaced fragment rather complex. If the Achilles tendon disruption is only discovered later once the fracture is healed, subsequent management is difficult with surgical treatment being more morbid. While this is a rare injury, the consequences of a missed chronic Achilles tendon disruption are severe with significant dysfunction. It is therefore important to have a high index of suspicion for concomitant injury and to be prepared for dual fixation. We present a novel hybrid surgical fixation technique, which may be used in this instance.

  7. Estimation of radon concentrations in coal mines using a hybrid technique calibration curve

    Energy Technology Data Exchange (ETDEWEB)

    Jamil, K.; Ali, S. [PINSTECH, Islamabad (Pakistan). Radiation Physics Division, Environmental Radiation Group

    2001-07-01

    A calibration curve was generated using Ra-226 activity concentration measured by a HPGe detector-based gamma-ray spectrometer versus alpha-track-density rate due to radon and its progeny on CR-39 track detector. Using the slops of the experimentally determined curve in the units of Becquerel per kilogram (Bq kg{sup -1}) per unit alpha -track-density per hour (cm{sup -2}h{sup -1}), radon concentrations (Bq m{sup -3}) were estimated using coal samples from various coal mines in two provinces of Pakistan, Punjab and Balochistan. Consequently, radon dose rates were computed in the simulated environment of the coal mines. These results provide only a screening method to indicate the radon dose in coal mines, It was shown that the actual measurements of radon concentrations in the coal mines are in agreement with the estimated radon concentrations using the hybrid-technique calibration curve.

  8. A millisecond micro-RNA separation technique by a hybrid structure of nanopillars and nanoslits

    Science.gov (United States)

    Wu, Qiong; Kaji, Noritada; Yasui, Takao; Rahong, Sakon; Yanagida, Takeshi; Kanai, Masaki; Nagashima, Kazuki; Tokeshi, Manabu; Kawai, Tomoji; Baba, Yoshinobu

    2017-01-01

    A millisecond micro-RNA separation of a mixture of total RNA and genomic DNA, extracted from cultured HeLa cells, was successfully achieved using a hybrid structure of nanopillars and nanoslits contained inside a microchannel. The nanopillars, 250-nm in diameter and 100-nm in height, were fabricated with a 750-nm space inside the nanoslits, which were 100-nm in height and 25-μm in width; the nanopillars were then applied as a new sieve matrix. This ultra-fast technique for the separation of miRNA can be an effective pretreatment for semiconductor nanopore DNA sequencing, which has an optimum reading speed of 1 base/ms to obtain effective signal-to-noise ratio and discriminate each base by ion or tunneling current during the passage of nucleic acids. PMID:28272420

  9. Fluorescence in situ hybridization for the tissue detection of bacterial pathogens associated with porcine infections

    DEFF Research Database (Denmark)

    Jensen, Henrik Elvang; Jensen, Louise Kruse; Barington, Kristiane

    2015-01-01

    sequences within intact cells. FISH allows direct histological localization of the bacteria in the tissue and thereby a correlation between the infection and the histopathological changes present. This chapter presents protocols for FISH identification of bacterial pathogens in fixed deparaffinized tissue......Fluorescence in situ hybridization (FISH) is an efficient technique for the identification of specific bacteria in tissue of both experimental and spontaneous infections. The method detects specific sequences of nucleic acids by hybridization of fluorescently labeled probes to complementary target...

  10. Fluorescence In Situ Hybridization for the Tissue Detection of Bacterial Pathogens Associated with Porcine Infections

    DEFF Research Database (Denmark)

    Elvang Jensen, Henrik; Jensen, Louise Kruse; Barington, Kristiane

    2015-01-01

    sequences within intact cells. FISH allows direct histological localization of the bacteria in the tissue and thereby a correlation between the infection and the histopathological changes present. This chapter presents protocols for FISH identification of bacterial pathogens in fixed deparaffinized tissue......Fluorescence in situ hybridization (FISH) is an efficient technique for the identification of specific bacteria in tissue of both experimental and spontaneous infections. The method detects specific sequences of nucleic acids by hybridization of fluorescently labeled probes to complementary target...

  11. A hybrid technique for private location-based queries with database protection

    KAUST Repository

    Ghinita, Gabriel

    2009-01-01

    Mobile devices with global positioning capabilities allow users to retrieve points of interest (POI) in their proximity. To protect user privacy, it is important not to disclose exact user coordinates to un-trusted entities that provide location-based services. Currently, there are two main approaches to protect the location privacy of users: (i) hiding locations inside cloaking regions (CRs) and (ii) encrypting location data using private information retrieval (PIR) protocols. Previous work focused on finding good trade-offs between privacy and performance of user protection techniques, but disregarded the important issue of protecting the POI dataset D. For instance, location cloaking requires large-sized CRs, leading to excessive disclosure of POIs (O(|D|) in the worst case). PIR, on the other hand, reduces this bound to , but at the expense of high processing and communication overhead. We propose a hybrid, two-step approach to private location-based queries, which provides protection for both the users and the database. In the first step, user locations are generalized to coarse-grained CRs which provide strong privacy. Next, a PIR protocol is applied with respect to the obtained query CR. To protect excessive disclosure of POI locations, we devise a cryptographic protocol that privately evaluates whether a point is enclosed inside a rectangular region. We also introduce an algorithm to efficiently support PIR on dynamic POI sub-sets. Our method discloses O(1) POI, orders of magnitude fewer than CR- or PIR-based techniques. Experimental results show that the hybrid approach is scalable in practice, and clearly outperforms the pure-PIR approach in terms of computational and communication overhead. © 2009 Springer Berlin Heidelberg.

  12. An Alternate Vista in Rehabilitation of Cranial Defects: Combining Digital and Manual Techniques to Fabricate a Hybrid Cranioplast.

    Science.gov (United States)

    Kaur, Harsimran; Nanda, Aditi; Koli, Dheeraj; Verma, Mahesh; Singh, Hukum; Bishnoi, Ishu; Pathak, Pooja; Gupta, Ankur

    2015-06-01

    The desired features of a cranioplast include providing an acceptable contour, continuity with the remaining skull (marginal adaptation), improvising the aesthetic outcome, providing a strengthened prosthesis to avoid fracture in case of repeat trauma, and protecting the remaining neurological structures. Combining digital and manual techniques to fabricate a hybrid polymethylmethacrylate cranioplast during the rehabilitation of a pediatric patient with cranial defect has been described. Utilization of digital techniques (rapid prototyping to obtain skull analog) and manual (hand) sculpting of the prosthesis strengthened with glass fiber enabled the authors to fabricate a hybrid cranioplast. Satisfactory outcome was achieved.

  13. Comparative study of different chromatographic techniques for the analysis of multi-residues of some approved antimicrobials in fish tissues.

    Science.gov (United States)

    Riad, Safa'a M; Rezk, Mamdouh R; Khattab, Fatma I; Marzouk, Hoda M

    2015-01-01

    Two chromatographic methods were developed, optimized and validated for the simultaneous determination of three approved aquaculture antimicrobials, namely sulphadimethoxine sodium, trimethoprim and florphenicol in fish tissues. The developed methods were based on simple liquid extraction technique. The first method employs thin-layer chromatography as a clean-up procedure coupled with densitometric determination for the separated drugs. The second method is an HPLC one using X-Terra™ C18 column. Several mobile-phase systems and extracting solvents were tried to optimize the separation and the extraction procedures from fish tissues. The procedures were applied for the analysis of spiked fish tissue samples at three different concentration levels (10, 50 and 100 ppm). A comparative study was conducted between the proposed methods to discuss the advantage of each one. The methods were validated according to the international conference on harmonization guidelines. The proposed methods were successfully applied for the determination of the studied drugs in spiked fish tissues, pure powders and in their veterinary pharmaceutical formulation.

  14. A novel fluorescent in situ hybridization technique for detection of Rickettsia spp. in archival samples

    DEFF Research Database (Denmark)

    Svendsen, Claus Bo; Boye, Mette; Struve, Carsten

    2009-01-01

    A novel, sensitive and specific method for detecting Rickettsia spp. in archival samples is described. The method involves the use of fluorescently marked oligonucleotide probes for in situ hybridization. Specific hybridization of Ricekttsia was found without problems of cross-reactions with bact......A novel, sensitive and specific method for detecting Rickettsia spp. in archival samples is described. The method involves the use of fluorescently marked oligonucleotide probes for in situ hybridization. Specific hybridization of Ricekttsia was found without problems of cross...

  15. FishPopTrace: a new genetic technique for fisheries monitoring and the identification of IUU

    DEFF Research Database (Denmark)

    Helyar, Sarah; Limborg, Morten; Bekkevold, Dorte

    2012-01-01

    of natural variation at a genetic level is limited. For marine fish, the maintenance of local stocks containing adaptive diversity is associated with the sustainability and resilience of marine fisheries in the face of climatic and anthropogenic threats. However, many previous genetic studies have observed...... weak genetic structure in marine fish and, combined with a pelagic larval stage, this has supported the hypothesis that gene flow is extensive and that there is little opportunity for differentiation and local adaptation any scale other than macrogeographic. However, the application of single...

  16. mRNA-targeted Fluorescent in Situ Hybridization (FISH) of Gram-negative Bacteria Without Template Amplification or Tyramide Signal Amplification

    Energy Technology Data Exchange (ETDEWEB)

    Coleman, James R.; Culley, David E.; Chrisler, William B.; Brockman, Fred J.

    2007-12-01

    Technologies are needed to study gene expression at the level of individual cells within a population or microbial community. Fluorescent in situ hybridization (FISH) supplies high-resolution spatial information and has been widely applied to study microbial communities at the rRNA level. While mRNA-targeted FISH has been popular for studying gene expression in eukaryotic cells, very little success has been achieved with prokaryotes. At present, detection of specific mRNAs in individual prokaryotic cells requires the use of in situ-RT-PCR or tyramide signal amplification (TSA). In this study we used DNA oligonucleotide probes labeled with a single near-infrared dye in FISH assays to detect multicopy plasmid-based and endogenous mRNA molecules in Escherichia coli and Shewanella oneidensis MR-1. We took advantage of the fact there is much less background signal produced by biological materials and support matrices in the near-infrared spectrum and thus long camera exposure times could be used. In addition, we demonstrate that a combination of probes targeting both rRNA and mRNA could be successfully employed within the same FISH assay. These results, as well as ongoing R&D improvements in NIR and infrared dyes, indicate the FISH approach we demonstrated could be applied in certain environmental settings to monitor gene expression in mixed populations.

  17. Detection of MDM2/CDK4 amplification in lipomatous soft tissue tumors from formalin-fixed, paraffin-embedded tissue: comparison of multiplex ligation-dependent probe amplification (MLPA) and fluorescence in situ hybridization (FISH).

    Science.gov (United States)

    Creytens, David; van Gorp, Joost; Ferdinande, Liesbeth; Speel, Ernst-Jan; Libbrecht, Louis

    2015-02-01

    In this study, the detection of MDM2 and CDK4 amplification was evaluated in lipomatous soft tissue tumors using multiplex ligation-dependent probe amplification (MLPA), a PCR-based technique, in comparison with fluorescence in situ hybridization (FISH). These 2 techniques were evaluated in a series of 77 formalin-fixed, paraffin-embedded lipomatous tumors (27 benign adipose tumors, 28 atypical lipomatous tumors/well-differentiated liposarcomas, 18 dedifferentiated liposarcomas, and 4 pleomorphic liposarcomas). Using MLPA, with a cut-off ratio of >2, 36/71 samples (22 atypical lipomatous tumors/well-differentiated liposarcomas, and 14 dedifferentiated liposarcomas) showed MDM2 and CDK4 amplification. Using FISH as gold standard, MLPA showed a sensitivity of 90% (36/40) and a specificity of 100% (31/31) in detecting amplification of MDM2 and CDK4 in lipomatous soft tissue tumors. In case of high-level amplification (MDM2-CDK4/CEP12 ratio >5), concordance was 100%. Four cases of atypical lipomatous tumor/well-differentiated liposarcoma (4/26, 15%) with a low MDM2 and CDK4 amplification level (MDM2-CDK4/CEP12 ratio ranging between 2 and 2.5) detected by FISH showed no amplification by MLPA, although gain of MDM2 and CDK4 (ratios ranging between 1.6 and 1.9) was seen with MLPA. No amplification was detected in benign lipomatous tumors and pleomorphic liposarcomas. Furthermore, there was a very high concordance between the ratios obtained by FISH and MLPA. In conclusion, MLPA proves to be an appropriate and straightforward technique for screening MDM2/CDK4 amplification in lipomatous tumors, especially when a correct cut-off value and reference samples are chosen, and could be considered a good alternative to FISH to determine MDM2 and CDK4 amplification in liposarcomas. Moreover, because MLPA, as a multiplex technique, allows simultaneous detection of multiple chromosomal changes of interest, it could be in the future a very reliable and fast molecular analysis on

  18. Organic Scintillators in Nonproliferation Applications With a Hybridized Double-Pulse Rejection Technique

    Science.gov (United States)

    Bourne, Mark Mitchell

    Alternative detection technologies are crucial to meeting demand for neutron detectors, for the current production of He-3, which has been the classical neutron choice, is insufficient. Organic scintillators are a strong candidate as a He-3 alternative due to their high efficiency, fast timing properties, and capabilities for separately identifying gamma-rays and neutrons through pulse shape discrimination (PSD). However, the use of organic scintillators in environments with numerous gamma rays can be limited because overlapping gamma-ray events can be misclassified as neutron events during PSD. To solve this problem, a new, hybridized double-pulse cleaning technique, consisting of three separate cleaning algorithms, was developed. The technique removes gamma-ray double pulses while preserving as many neutron pulses as possible. This technique was applied to separate experiments of Cf-252 and a gamma-ray source when measuring at a 100-kHz count rate and a field of 1000 incident gamma rays per incident neutron. It was found that stilbene scintillators were capable of intrinsic neutron efficiencies between 15-19% when measuring bare Cf-252 and 13-17% when exposed to the gamma-ray field. Misclassification rates ranged from 10-6-10-5, a factor-of-5 better than both the EJ-309 liquid and BB3-5 plastic. Next, plutonium experiments were performed with stilbene to determine which cleaning algorithm was best for each sample. A clear correlation was found that related the correct method of cleaning to the measured gamma ray-to-neutron ratio. When the measured gamma ray-to-neutron ratio is 10 or below, the template cleaning algorithm is preferred, while the fractional and hybrid cleaning algorithms are preferred when the gamma ray-to-neutron ratio is 100 or greater. Discriminating neutron sources such as Cf-252 or AmLi from SNM samples such as plutonium is a top priority in nonproliferation. We demonstrate that time-correlated experiments, utilizing both PSD-capable plastic

  19. A Discrete Wavelet Based Feature Extraction and Hybrid Classification Technique for Microarray Data Analysis

    Directory of Open Access Journals (Sweden)

    Jaison Bennet

    2014-01-01

    Full Text Available Cancer classification by doctors and radiologists was based on morphological and clinical features and had limited diagnostic ability in olden days. The recent arrival of DNA microarray technology has led to the concurrent monitoring of thousands of gene expressions in a single chip which stimulates the progress in cancer classification. In this paper, we have proposed a hybrid approach for microarray data classification based on nearest neighbor (KNN, naive Bayes, and support vector machine (SVM. Feature selection prior to classification plays a vital role and a feature selection technique which combines discrete wavelet transform (DWT and moving window technique (MWT is used. The performance of the proposed method is compared with the conventional classifiers like support vector machine, nearest neighbor, and naive Bayes. Experiments have been conducted on both real and benchmark datasets and the results indicate that the ensemble approach produces higher classification accuracy than conventional classifiers. This paper serves as an automated system for the classification of cancer and can be applied by doctors in real cases which serve as a boon to the medical community. This work further reduces the misclassification of cancers which is highly not allowed in cancer detection.

  20. Progress in molecular diagnosis of Charcot-Marie-Tooth-disease type 1 (CMT 1, HMSN I) and hereditary neuropathy with liability to pressure palsies (HNPP) by fluorescence in situ hybridization (FISH)-detection of a potential genetic mosaicism

    Energy Technology Data Exchange (ETDEWEB)

    Bathke, K.; Liehr. T.; Ekici, A. [Institute for Human Genetics, Erlange (Germany)] [and others

    1994-09-01

    We tested 20 CMT 1 patients characterized according to the criteria of the European CMT consortium by Southern hybridization of MspI restricted genomic DNA with probes pVAW409R1, pVAW412Hec and pEW401HE. In 11 of the 20 CMT 1 cases (55%), we observed a duplication in 17q11.2; one patient had a dinucleotide insertion in exon 6 of the PO-gene (5%). One HNPP case had a typical 17p11.2 deletion. Analysis of CA-repeats was performed with primers RM11GT and Mfd41; SSCP-analysis of the PO, PMP22 and Cx32-genes is in progress. FISH was carried out with probe pVAW409R1. 125 interphase nuclei were analyzed for each proband by counting the signals per nucleus. Normal cells show a characteristic distribution of signals: 1 signal in 5.9% of nuclei, 2 in 86.3% and 3 in 7.8%. A duplication is indicated by a shift to 3 signals in more than approximately 60% and 2 in less than 25% of the nuclei. In contrast, the 17p11.2 deletion of the HNPP patient shifts to 82.4% of nuclei with a single hybridization signal versus 14.4% with 2 signals. We detected one case with significantly abnormal distribution of interphase nuclei hybridization signals compared to cultures of normal cells and to those with 17p11.2 duplication or deletion: 3.2% nuclei revealed 1 signal, 48.0% two signals and 48.8% 3 signals, indicating a pathogenic but moderate dosis increase compared to the throughout duplicated cases. FISH with probe pVAW409R1 is a versatile tool to detect the HNPP deletion both in interphase nuclei and in metaphase chromosomes. In CMT 1 disease interphase nuclei are required for FISH analysis due to the small duplication of 1.5 Mbp. In contrast to Southern techniques, FISH is able to detect genetic mosaicism.

  1. Numbers and locations of native bacteria on field-grown wheat roots quantified by fluorescence in situ hybridization (FISH).

    Science.gov (United States)

    Watt, Michelle; Hugenholtz, Philip; White, Rosemary; Vinall, Kerry

    2006-05-01

    Native bacteria, Pseudomonas and filamentous bacteria were quantified and localized on wheat roots grown in the field using fluorescence in situ hybridization (FISH). Seminal roots were sampled through the season from unploughed soil in a conservation farming system. Such soils are spatially heterogeneous, and many roots grow slowly through hard soil with cracks and pores containing dead roots remnant from previous crops. Root and rhizosphere morphology, and contact with soil particles were preserved, and autofluorescence was avoided by observing sections in the far-red with Cy5 and Cy5.5 fluorochromes. Spatial analyses showed that bacteria were embedded in a stable matrix (biofilm) within 11 microm of the root surface (range 2-30 microm) and were clustered on 40% of roots. Half the clusters co-located with axial grooves between epidermal cells, soil particles, cap cells or root hairs; the other half were not associated with visible features. Across all wheat roots, although variable, bacteria averaged 15.4 x 10(5) cells per mm(3) rhizosphere, and of these, Pseudomonas and filaments comprised 10% and 4%, respectively, with minor effects of sample time, and no effect of plant age. Root caps were most heavily colonized by bacteria along roots, and elongation zones least heavily colonized. Pseudomonas varied little with root development and were 17% of bacteria on the elongation zone. Filamentous bacteria were not found on the elongation zone. The most significant factor to rhizosphere populations along a wheat root, however, was contact with dead root remnants, where Pseudomonas were reduced but filaments increased to 57% of bacteria (P < 0.001). This corresponded with analyses of root remnants showing they were heavily colonized by bacteria, with 48% filaments (P < 0.001) and 1.4%Pseudomonas (P = 0.014). Efforts to manage rhizosphere bacteria for sustainable agricultural systems should continue to focus on root cap and mucilage chemistry, and remnant roots as

  2. DVC-FISH and PMA-qPCR techniques to assess the survival of Helicobacter pylori inside Acanthamoeba castellanii.

    Science.gov (United States)

    Moreno-Mesonero, Laura; Moreno, Yolanda; Alonso, José Luis; Ferrús, M Antonia

    2016-01-01

    Free-living amoebae (FLA) are ubiquitous microorganisms commonly found in water. They can act as Trojan Horses for some amoeba-resistant bacteria (ARB). Helicobacter pylori is a pathogenic bacteria, suggested to be transmitted through water, which could belong to the ARB group. In this work, a co-culture assay of H. pylori and Acanthamoeba castellanii, one of the most common FLA, was carried out to identify the presence and survival of viable and potentially infective forms of the bacteria internalized by the amoeba. Molecular techniques including FISH, DVC-FISH, qPCR and PMA-qPCR were used to detect the presence of internalized and viable H. pylori. After 24 h in co-culture and disinfection treatment to kill extra-amoebic bacteria, viable H. pylori cells were observed inside A. castellanii. When PMA-qPCR was applied to the co-culture samples, only DNA from internalized H. pylori cells was detected, whereas qPCR amplified total DNA from the sample. By the combined DVC-FISH method, the viability of H. pylori cells in A. castellanii was observed. Both specific techniques provided evidence, for the first time, that the pathogen is able to survive chlorination treatment in occurrence with A. castellanii and could be very useful methods for performing further studies in environmental samples.

  3. A Polar Fuzzy Control Scheme for Hybrid Power System Using Vehicle-To-Grid Technique

    Directory of Open Access Journals (Sweden)

    Mohammed Elsayed Lotfy

    2017-07-01

    Full Text Available A novel polar fuzzy (PF control approach for a hybrid power system is proposed in this research. The proposed control scheme remedies the issues of system frequency and the continuity of demand supply caused by renewable sources’ uncertainties. The hybrid power system consists of a wind turbine generator (WTG, solar photovoltaics (PV, a solar thermal power generator (STPG, a diesel engine generator (DEG, an aqua-electrolyzer (AE, an ultra-capacitor (UC, a fuel-cell (FC, and a flywheel (FW. Furthermore, due to the high cost of the battery energy storage system (BESS, a new idea of vehicle-to-grid (V2G control is applied to use the battery of the electric vehicle (EV as equivalent to large-scale energy storage units instead of small batteries to improve the frequency stability of the system. In addition, EV customers’ convenience is taken into account. A minimal-order observer is used to estimate the supply error. Then, the area control error (ACE signal is calculated in terms of the estimated supply error and the frequency deviation. ACE is considered in the frequency domain. Two PF approaches are utilized in the intended system. The mission of each controller is to mitigate one frequency component of ACE. The responsibility for ACE compensation is shared among all parts of the system according to their speed of response. The performance of the proposed control scheme is compared to the conventional fuzzy logic control (FLC. The effectiveness and robustness of the proposed control technique are verified by numerical simulations under various scenarios.

  4. Fluorescence in situ hybridization and sequential catalysed reporter deposition (2C-FISH for the flow cytometric sorting of freshwater ultramicrobacteria

    Directory of Open Access Journals (Sweden)

    Stefan M Neuenschwander

    2015-03-01

    Full Text Available Flow cytometric sorting is a powerful tool to physically separate cells within mixed microbial communities. If combined with phylogenetic staining (fluorescence in situ hybridization, FISH it allows to specifically sort defined genotypic microbial populations from complex natural samples. However, the targeted enrichment of freshwater ultramicrobacteria, such as members of the LD12 clade of Alphaproteobacteria (SAR11-IIIb, is still challenging. Current FISH protocols, even in combination with signal amplification by catalysed reporter deposition (CARD, are not sufficiently sensitive for the distinction of these bacteria from background noise by flow cytometry, presumably due to their low ribosome content and small cell sizes. We, therefore, modified a CARD based flow sorting protocol with the aim of increasing its sensitivity to a level sufficient for ultramicrobacteria. This was achieved by a second signal amplification step mediated by horseradish peroxidase labelled antibodies targeted to the fluorophores that were previously deposited by CARD-FISH staining. The protocol was tested on samples from an oligo-mesotrophic lake. Ultramicrobacteria affiliated with LD12 Alphaproteobacteria could be successfully sorted to high purity by flow cytometry. The ratios of median fluorescence signal to background ranged around 20, and hybridization rates determined by flow cytometry were comparable to those obtained by fluorescence microscopy. Potential downstream applications of our modified cell staining approach range from the analysis of microdiversity within 16S rRNA-defined populations to that of functional properties, such as the taxon-specific incorporation rates of organic substrates.

  5. Silica/polyacrylonitrile hybrid nanofiber membrane separators via sol-gel and electrospinning techniques for lithium-ion batteries

    Science.gov (United States)

    Yanilmaz, Meltem; Lu, Yao; Zhu, Jiadeng; Zhang, Xiangwu

    2016-05-01

    Silica/polyacrylonitrile (SiO2/PAN) hybrid nanofiber membranes were fabricated by using sol-gel and electrospinning techniques and their electrochemical performance was evaluated for use as separators in lithium-ion batteries. The aim of this study was to design high-performance separator membranes with enhanced electrochemical performance and good thermal stability compared to microporous polyolefin membranes. In this study, SiO2 nanoparticle content up to 27 wt% was achieved in the membranes by using sol-gel technique. It was found that SiO2/PAN hybrid nanofiber membranes had superior electrochemical performance with good thermal stability due to their high SiO2 content and large porosity. Compared with commercial microporous polyolefin membranes, SiO2/PAN hybrid nanofiber membranes had larger liquid electrolyte uptake, higher electrochemical oxidation limit, and lower interfacial resistance with lithium. SiO2/PAN hybrid nanofiber membranes with different SiO2 contents (0, 16, 19 and 27 wt%) were also assembled into lithium/lithium iron phosphate cells, and high cell capacities and good cycling performance were demonstrated at room temperature. In addition, cells using SiO2/PAN hybrid nanofiber membranes with high SiO2 contents showed superior C-rate performance compared to those with low SiO2 contents and commercial microporous polyolefin membrane.

  6. Magnetic Fe3O4/Ag Hybrid Nanoparticles as Surface-Enhanced Raman Scattering Substrate for Trace Analysis of Furazolidone in Fish Feeds

    Directory of Open Access Journals (Sweden)

    Wansong Yu

    2014-01-01

    Full Text Available Nanoparticles (NPs composed of ferromagnetic and noble metal materials show dual functions of magnetic activity and local surface plasmon response and have great potential as substrates for surface-enhanced Raman scattering (SERS in trace analysis. Easy-to-prepare superparamagnetic Fe3O4/Ag hybrid NPs were synthesized and optimized by adjusting the ratio of silver particles aggregated with APTMS-modified Fe3O4 NPs. The hybrid NPs were assembled under an external magnetic field before being used as substrate for SERS analysis. The SERS spectral features of furazolidone standard solution were clearly identified at concentrations as low as 40 ng mL−1, and furazolidone in fish feeds could be detected at 500 ng g−1. The results indicated that the Fe3O4/Ag hybrid NPs as SERS substrates had a great potential for detection of trace amount of furazolidone and other prohibited or restricted antibiotics in the animal and fish feeds.

  7. speciation through asexuality in fish: postzygotic reproductive isolation may be completed in spite of fertility of hybrids

    Directory of Open Access Journals (Sweden)

    Karel Janko

    2015-12-01

    Altogether, it appears that initiation of hybrid asexuality and the completion of speciation process through formation of postRIMs are interconnected phenomena. Both processes are linked to the genetic divergence of hybridizing taxa: initially, hybridization between little diverged species leads to recombinant and fertile hybrids allowing intensive gene flow. As the hybridizing taxa continue to diverge, clonally reproducing hybrid females and sterile males become dominant and the gene flow ceases. The speciation may therefore be completed through asexuality of hybrids The work was supported by grant no. 13-12580S provided by the Czech Science Foundation (www.gacr.cz. Further support was provided by the Academy of Sciences of the Czech Republic (www.cas.cz by the grant no. RVO 67985904

  8. A novel hybrid meta-heuristic technique applied to the well-known benchmark optimization problems

    Science.gov (United States)

    Abtahi, Amir-Reza; Bijari, Afsane

    2016-09-01

    In this paper, a hybrid meta-heuristic algorithm, based on imperialistic competition algorithm (ICA), harmony search (HS), and simulated annealing (SA) is presented. The body of the proposed hybrid algorithm is based on ICA. The proposed hybrid algorithm inherits the advantages of the process of harmony creation in HS algorithm to improve the exploitation phase of the ICA algorithm. In addition, the proposed hybrid algorithm uses SA to make a balance between exploration and exploitation phases. The proposed hybrid algorithm is compared with several meta-heuristic methods, including genetic algorithm (GA), HS, and ICA on several well-known benchmark instances. The comprehensive experiments and statistical analysis on standard benchmark functions certify the superiority of the proposed method over the other algorithms. The efficacy of the proposed hybrid algorithm is promising and can be used in several real-life engineering and management problems.

  9. A novel hybrid meta-heuristic technique applied to the well-known benchmark optimization problems

    Science.gov (United States)

    Abtahi, Amir-Reza; Bijari, Afsane

    2017-09-01

    In this paper, a hybrid meta-heuristic algorithm, based on imperialistic competition algorithm (ICA), harmony search (HS), and simulated annealing (SA) is presented. The body of the proposed hybrid algorithm is based on ICA. The proposed hybrid algorithm inherits the advantages of the process of harmony creation in HS algorithm to improve the exploitation phase of the ICA algorithm. In addition, the proposed hybrid algorithm uses SA to make a balance between exploration and exploitation phases. The proposed hybrid algorithm is compared with several meta-heuristic methods, including genetic algorithm (GA), HS, and ICA on several well-known benchmark instances. The comprehensive experiments and statistical analysis on standard benchmark functions certify the superiority of the proposed method over the other algorithms. The efficacy of the proposed hybrid algorithm is promising and can be used in several real-life engineering and management problems.

  10. Irradiation for dating Brazilian fish fossil by thermoluminescence and EPR technique

    Energy Technology Data Exchange (ETDEWEB)

    Sullasi, H.S.; Andrade, M.B.; Ayta, W.E.F.; Frade, M.; Sastry, M.D.; Watanabe, S

    2004-01-01

    Fish fossil from Ceara State, Brazil has been investigated using thermoluminescence and EPR method. In both cases, additive method has been used by irradiating fossil samples to very high doses (tens of kGy). In the case of thermoluminescence, 360 deg. C peak was used for dating. Since the fish fossil contains relatively high concentration of Mn, the EPR Mn{sup 2+}-lines cover carbonate and sulfate radicals signal (sulfur is also present in large amount), therefore 50 mW microwave power was used for EPR measurements. At this high power region Mn{sup 2+}-lines become very little intense and SO{sub 2}{sup -} and CO{sub 2}{sup -} can be detected. Both TL and EPR dating presented an age around 8 Ma. Correction due to spontaneous decay of 360 deg. C peak at ambient temperature gives rise to about {approx}24 Ma of age.

  11. An anchoring system for fish habitat structures: field technique, evaluation, and application.

    Science.gov (United States)

    Barbara L. Fontaine; Thomas D. Merritt

    1988-01-01

    Steel cable can be used to bind rocks and logs together to construct fish habitat structures in streams. Cables must be securely anchored if structures are to withstand floods. This paper describes a way to anchor cables into bedrock or ballast boulders. Anchor tensile strength ranged from 7,500 to 36,500 pounds and was related to type of resin and embedment depth....

  12. An industry-scale mass marking technique for tracing farmed fish escapees.

    Directory of Open Access Journals (Sweden)

    Fletcher Warren-Myers

    Full Text Available Farmed fish escape and enter the environment with subsequent effects on wild populations. Reducing escapes requires the ability to trace individuals back to the point of escape, so that escape causes can be identified and technical standards improved. Here, we tested if stable isotope otolith fingerprint marks delivered during routine vaccination could be an accurate, feasible and cost effective marking method, suitable for industrial-scale application. We tested seven stable isotopes, (134Ba, (135Ba, (136Ba, (137Ba, (86Sr, (87Sr and (26Mg, on farmed Atlantic salmon reared in freshwater, in experimental conditions designed to reflect commercial practice. Marking was 100% successful with individual Ba isotopes at concentrations as low as 0.001 µg. g-1 fish and for Sr isotopes at 1 µg. g-1 fish. Our results suggest that 63 unique fingerprint marks can be made at low cost using Ba (0.0002 - 0.02 $US per mark and Sr (0.46 - 0.82 $US per mark isotopes. Stable isotope fingerprinting during vaccination is feasible for commercial application if applied at a company level within the world's largest salmon producing nations. Introducing a mass marking scheme would enable tracing of escapees back to point of origin, which could drive greater compliance, better farm design and improved management practices to reduce escapes.

  13. An industry-scale mass marking technique for tracing farmed fish escapees.

    Science.gov (United States)

    Warren-Myers, Fletcher; Dempster, Tim; Fjelldal, Per Gunnar; Hansen, Tom; Swearer, Stephen E

    2015-01-01

    Farmed fish escape and enter the environment with subsequent effects on wild populations. Reducing escapes requires the ability to trace individuals back to the point of escape, so that escape causes can be identified and technical standards improved. Here, we tested if stable isotope otolith fingerprint marks delivered during routine vaccination could be an accurate, feasible and cost effective marking method, suitable for industrial-scale application. We tested seven stable isotopes, (134)Ba, (135)Ba, (136)Ba, (137)Ba, (86)Sr, (87)Sr and (26)Mg, on farmed Atlantic salmon reared in freshwater, in experimental conditions designed to reflect commercial practice. Marking was 100% successful with individual Ba isotopes at concentrations as low as 0.001 µg. g-1 fish and for Sr isotopes at 1 µg. g-1 fish. Our results suggest that 63 unique fingerprint marks can be made at low cost using Ba (0.0002 - 0.02 $US per mark) and Sr (0.46 - 0.82 $US per mark) isotopes. Stable isotope fingerprinting during vaccination is feasible for commercial application if applied at a company level within the world's largest salmon producing nations. Introducing a mass marking scheme would enable tracing of escapees back to point of origin, which could drive greater compliance, better farm design and improved management practices to reduce escapes.

  14. Comparison of immunohistochemistry (IHC and fluorescence in situ hybridization (FISH assessment for Her-2 status in breast cancer

    Directory of Open Access Journals (Sweden)

    Qi Minfang

    2009-11-01

    Full Text Available Abstract Background The concordance rate between IHC and FISH according to clinical performance is still controversial. We report a prospective study to reflect the concordance between IHC and FISH in Guilin city, People's Republic of China. Methods Fifty cases of invasive ductal carcinoma of breast tested by IHC and scored as 0, 1+, 2+ and 3+ by pathologists were further analyzed by FISH using a commercially available double-color probe, and the FISH findings were compared with IHC test results. Results A total concordance of 82.0% was observed with a Kappa coefficient of 0.640 (P Conclusion The IHC can be used firstly to screen the HER-2 status, and FISH can be used as a supplementary role to IHC and 2+ and some negative cases. And only those cases with Her-2 status of IHC 3+ or FISH positive should be treated with Herceptin.

  15. A hybrid PSO technique for procuring VAR ancillary service in the deregulated electricity markets

    Energy Technology Data Exchange (ETDEWEB)

    El-Araby, E.E. [Department of Electrical Engineering, Suez Canal University (Egypt); Yorino, Naoto [Department of Artificial Complex Systems Engineering, Hiroshima University (Japan)

    2010-07-15

    This paper develops a new market-based technique for acquiring VAR ancillary service in the electricity market. The main objective of the developed market is to enable transmission operator ''TO'' to procure VAR service in a long term contract from the critical VAR providers that satisfy minimum VAR service payment while maintaining system security. Reactive power control problem for voltage stability is introduced into the VAR market problem in an explicit manner for normal and emergency states. An integration of particle swarm optimization ''PSO'' is presented with successive linear programming ''SLP'' for dealing with the VAR ancillary service problem. The problem is formulated as a large-scale nonlinear constrained optimization problem with a non-differentiable objective function representing VAR payment and operational costs. This type of problem is hard to be treated straightforwardly by the classical optimization methods. Therefore, we propose here a two-layer hybrid PSO/SLP approach, which is suited for carrying out the difficulties associated with non-differentiable and discontinuous objective functions. The proposed method has been examined on the standard IEEE 57 bus-system and compared with GA/SLP method to demonstrate its capability. (author)

  16. Optimization of RF power absorption by optimization techniques using the lower hybrid current drive of FTU

    Energy Technology Data Exchange (ETDEWEB)

    Centioli, C. [Associazione Euratom/ENEA Sulla Fusione, Centro Ricerche Frascati, Via E. Fermi 45, CP 65, 00044 Frascati, Rome (Italy); Iannone, F. [Associazione Euratom/ENEA Sulla Fusione, Centro Ricerche Frascati, Via E. Fermi 45, CP 65, 00044 Frascati, Rome (Italy); Mazza, G. [Associazione Euratom/ENEA Sulla Fusione, Centro Ricerche Frascati, Via E. Fermi 45, CP 65, 00044 Frascati, Rome (Italy); Panella, M. [Associazione Euratom/ENEA Sulla Fusione, Centro Ricerche Frascati, Via E. Fermi 45, CP 65, 00044 Frascati, Rome (Italy); Pangione, L. [Dipartimento di Informatica, Sistemi e Produzione, Universita di Roma, Tor Vergata, Via del Politecnico 1, 00133 Rome (Italy)]. E-mail: pangione@frascati.enea.it; Podda, S. [Associazione Euratom/ENEA Sulla Fusione, Centro Ricerche Frascati, Via E. Fermi 45, CP 65, 00044 Frascati, Rome (Italy); Tuccillo, A. [Associazione Euratom/ENEA Sulla Fusione, Centro Ricerche Frascati, Via E. Fermi 45, CP 65, 00044 Frascati, Rome (Italy); Vitale, V. [Associazione Euratom/ENEA Sulla Fusione, Centro Ricerche Frascati, Via E. Fermi 45, CP 65, 00044 Frascati, Rome (Italy); Zaccarian, L. [Dipartimento di Informatica, Sistemi e Produzione, Universita di Roma, Tor Vergata, Via del Politecnico 1, 00133 Rome (Italy)

    2005-11-15

    In this paper, we will report on the experimental results arising from the implementation of optimization techniques to maximize the RF power coupling versus the plasma conditions in the FTU experimental facility. These experiments are carried out by employing the open-source Linux-RTAI control system currently running on the FTU digital feedback loop. The RF power source under consideration is a lower hybrid system (LH) based on six gyrotrons with a nominal power output capability of 1.1 MW each. The optimization of the coupling level between the plasma and the emitting antenna reduces the reflected power, thus maximizing the heating effects in addition to avoiding danger to the emitter (equivalently, annoying safety shutdowns of the system). To this aim, the plasma displacement is modified by suitably adjusting the reference input to the stabilizing feedback, according to a steepest descent algorithm. It will be shown in the paper how this algorithm achieves a satisfactory level of robustness with respect to measurement errors and well performs both in simulation and in experimental tests, thus leading to an improved effectiveness of the RF heating system.

  17. Hybrid LS-LMMSE Channel Estimation Technique for LTE Downlink Systems

    CERN Document Server

    Khlifi, Abdelhakim; 10.5121/ijngn.2011.3401

    2012-01-01

    In this paper, we propose to improve the performance of the channel estimation for LTE Downlink systems under the effect of the channel length. As LTE Downlink system is a MIMO-OFDMA based system, a cyclic prefix (CP) is inserted at the beginning of each transmitted OFDM symbol in order to mitigate both inter-carrier interference (ICI) and inter-symbol interference (ISI). The inserted CP is usually equal to or longer than the channel length. However, the cyclic prefix can be shorter because of some unforeseen channel behaviour. Previous works have shown that in the case where the cyclic prefix is equal to or longer than the channel length, LMMSE performs better than LSE but at the cost of computational complexity .In the other case, LMMSE performs also better than LS only for low SNR values. However, LS shows better performance for LTE Downlink systems for high SNR values. Therefore, we propose a hybrid LS-LMMSE channel estimation technique robust to the channel length effect. MATLAB Monte-Carlo simulations a...

  18. Power Management Based Current Control Technique for Photovoltaic-Battery Assisted Wind-Hydro Hybrid System

    Science.gov (United States)

    Ram Prabhakar, J.; Ragavan, K.

    2013-07-01

    This article proposes new power management based current control strategy for integrated wind-solar-hydro system equipped with battery storage mechanism. In this control technique, an indirect estimation of load current is done, through energy balance model, DC-link voltage control and droop control. This system features simpler energy management strategy and necessitates few power electronic converters, thereby minimizing the cost of the system. The generation-demand (G-D) management diagram is formulated based on the stochastic weather conditions and demand, which would likely moderate the gap between both. The features of management strategy deploying energy balance model include (1) regulating DC-link voltage within specified tolerances, (2) isolated operation without relying on external electric power transmission network, (3) indirect current control of hydro turbine driven induction generator and (4) seamless transition between grid-connected and off-grid operation modes. Furthermore, structuring of the hybrid system with appropriate selection of control variables enables power sharing among each energy conversion systems and battery storage mechanism. By addressing these intricacies, it is viable to regulate the frequency and voltage of the remote network at load end. The performance of the proposed composite scheme is demonstrated through time-domain simulation in MATLAB/Simulink environment.

  19. Fluorescence in situ hybridization (FISH using non-commercial probes in the diagnosis of clinically suspected microdeletion syndromes

    Directory of Open Access Journals (Sweden)

    Ashutosh Halder

    2013-01-01

    Interpretation & conclusion: We conclude that FISH should not be the method of choice for clinically suspected microdeletion syndromes. We propose to follow strict clinical criteria for FISH testing or preferably to follow better methods (genotype first approach. Whole genome screening may be used as first line of test and FISH may be used for confirmation of screening result, screening of family members and prenatal diagnosis.

  20. Modified techniques used for microsatellite and AFLP for the population study of divers species at the Sinu river fish, Colombia

    Directory of Open Access Journals (Sweden)

    Lamprea Natalia

    2004-07-01

    Full Text Available Few genetic conservation studies carried out in Colombia have applied molecular biology techniques. Protocols and modifications arising from the genetic study of four fish species from the Sinú river basin are presented as guidelines for future work in this field (and with other species as laboratory standardisation processes are complex. The original commercial kit's DNA extraction protocols were modified as were those for PCR reactions for obtaining micro-satellite markers and AFLPs, as well as allele genotypification. Particular variations were made f or each of the species studied. Key words: DNA extraction; molecular biology; genotypification; ichthyology.

  1. A populational survey of 45S rDNA polymorphism in the Jefferson salamander Ambystoma jeffersonianum revealed by fluorescence in situ hybridization (FISH)

    Institute of Scientific and Technical Information of China (English)

    Ke BI; James P.BOGART; Jinzhong FU

    2009-01-01

    The chromosomal localization of 45S ribosomal RNA genes in Ambystoma jeffersonianum was determined by fluorescence in situ hybridization with 18S rDNA fragment as a probe (FISH-rDNA). Our results revealed the presence of rDNA polymorphism among A.jeffersonianum populations in terms of number, location and FISH signal intensity on the chromosomes. Nine rDNA cytotypes were found in ten geographically isolated populations and most of them contained derivative rDNA sites. Our preliminary study provides strong indication of karyotypic diversification of A.jeffersonianum that is demonstrated by intraspecific variation of 45S rDNA cytotypes. rDNA cytotype polymorphism has been described in many other caudate amphibians. We predict that habitat isolation, low dispersal ability and decline of effective population size could facilitate the fixation and accumulation of variable rDNA cytotypes during their chromosome evolution [Current Zoology 55(2):145-149,2009].

  2. A populational survey of 45S rDNA polymorphism in the Jefferson salamander Ambystoma jeffersonianum revealed by fluorescence in situ hybridization (FISH

    Directory of Open Access Journals (Sweden)

    Jinzhong FU

    2009-04-01

    Full Text Available The chromosomal localization of 45S ribosomal RNA genes in Ambystoma jeffersonianum was determined by fluorescence in situ hybridization with 18S rDNA fragment as a probe (FISH-rDNA. Our results revealed the presence of rDNA polymorphism among A.jeffersonianum populations in terms of number, location and FISH signal intensity on the chromosomes. Nine rDNA cytotypes were found in ten geographically isolated populations and most of them contained derivative rDNA sites. Our preliminary study provides strong indication of karyotypic diversification of A.jeffersonianum that is demonstrated by intraspecific variation of 45S rDNA cytotypes. rDNA cytotype polymorphism has been described in many other caudate amphibians. We predict that habitat isolation, low dispersal ability and decline of effective population size could facilitate the fixation and accumulation of variable rDNA cytotypes during their chromosome evolution.

  3. Effects of the acute exposition to glyphosate-based herbicide on oxidative stress parameters and antioxidant responses in a hybrid Amazon fish surubim (Pseudoplatystoma sp).

    Science.gov (United States)

    Sinhorin, Valéria Dornelles Gindri; Sinhorin, Adilson Paulo; Teixeira, Jhonnes Marcos dos Santos; Miléski, Kelly Márcia Lazarotto; Hansen, Paula Carine; Moreira, Paula Sueli Andrade; Kawashita, Nair Honda; Baviera, Amanda Martins; Loro, Vania Lúcia

    2014-08-01

    The aim of this study was to investigate the effects of acute glyphosate (active ingredient) exposure on the oxidative stress biomarkers and antioxidant defenses of a hybrid surubim (Pseudoplatystoma sp). The fish were exposed to different herbicide concentrations for 96 h. The thiobarbituric acid-reactive substances (TBARS), protein carbonyls and antioxidant responses were verified. The 15 mg a.pL(-1) of herbicide resulted in the death of 50% of the fish after 96 h. An increase in liver and muscle TBARS levels was observed when fish were exposed to the herbicide. The protein carbonyl content was also increased in the liver (4.5mg a.pL(-1) concentration) and brain (2.25 mg a.pL(-1) concentration). The antioxidant activities decreased in the liver and brain after exposure to herbicide. Levels of ascorbic acid in the liver (2.25 mg a.pL(-1) and 4.5 mg a.pL(-1) concentrations) and brain (2.25 mg a.pL(-1) concentration) were increased post-treatment. Levels of total thiols were increased in the liver and brain (2.25 mg L(-1) and 7.5mg a.pL(-1), respectively). Glyphosate exposure, at the tested concentrations affects surubim health by promoting changes that can affect their survival in natural environment. Some parameters as TBARS and protein carbonyl could be early biomarkers for Roundup exposure in this fish species.

  4. Fluorescent in situ hybridization as an adjunct to conventional cytogenetics.

    Science.gov (United States)

    Mark, H F

    1994-01-01

    Fluorescent in situ hybridization (FISH) is a molecular cytogenetic technique that exploits the availability of recombinant deoxyribonucleic acid (DNA) technology. In metaphase FISH, a specific nucleic acid sequence (probe) is bound to the homologous segment on a metaphase chromosome in a fixed preparation on a glass slide. The presence of a region-specific DNA sequence in a nondividing cell can be detected using interphase FISH. Interphase cytogenetics via FISH can be performed on fixed cells harvested during a routine culture, on tissue sections and on many cytologic specimens. Specific examples of clinical and research applications are discussed to illustrate the utility of FISH in the detection of constitutional and acquired chromosomal abnormalities.

  5. Fluorescence in situ hybridization (FISH screening for the 22q11.2 deletion in patients with clinical features of velocardiofacial syndrome but without cardiac anomalies

    Directory of Open Access Journals (Sweden)

    Paula Sandrin-Garcia

    2007-01-01

    Full Text Available The velocardiofacial syndrome (VCFS, a condition associated with 22q11.2 deletions, is characterized by a typical facies, palatal anomalies, learning disabilities, behavioral disturbances and cardiac defects. We investigated the frequency of these chromosomal deletions in 16 individuals with VCFS features who presented no cardiac anomalies, one of the main characteristics of VCFS. Fluorescent in situ hybridization (FISH with the N25 (D22S75; 22q11.2 probe revealed deletions in ten individuals (62%. Therefore, even in the absence of cardiac anomalies testing for the 22q11.2 microdeletions in individuals showing other clinical features of this syndrome is recommended.

  6. Performance improvement of hybrid subcarrier multiplexing optical spectrum code division multiplexing system using spectral direct decoding detection technique

    Science.gov (United States)

    Sahbudin, R. K. Z.; Abdullah, M. K.; Mokhtar, M.

    2009-06-01

    This paper proposes a hybrid subcarrier multiplexing/optical spectrum code division multiplexing (SCM/OSCDM) system for the purpose of combining the advantages of both techniques. Optical spectrum code division multiple-access (OSCDMA) is one of the multiplexing techniques that is becoming popular because of the flexibility in the allocation of channels, ability to operate asynchronously, enhanced privacy and increased capacity in bursty nature networks. On the other hand, subcarrier multiplexing (SCM) technique is able to enhance the channel data rate of OSCDMA systems. In this paper, a newly developed detection technique for the OSCDM called spectral direct decoding (SDD) detection technique is compared mathematically with the AND subtraction detection technique. The system utilizes a new unified code construction named KS (Khazani-Syed) code. The results characterizing the bit-error-rate (BER) show that SDD offers a significant improved performance at BER of 10 -9.

  7. 荧光原位杂交法(FISH)快速检测尿液中金黄色葡萄球菌%Fluorescence in Situ Hybridization Rapidly Detects Staphylococcus Aureus in Urinary Tract Infection Samples

    Institute of Scientific and Technical Information of China (English)

    吴青; 李艳; 汪明; 顾剑; 胡慧霞; 徐万洲; 韦传东; 吴泽刚

    2011-01-01

    目的 利用荧光原位杂交法快速检测尿液中的金黄色葡萄球菌,筛查金黄色葡萄球菌所致的尿路感染.方法 针对金黄色葡萄球菌16sRNA设计荧光标记的核苷酸探针,利用荧光原位杂交技术(FISH)对132例疑似尿路感染患者中段尿标本进行检测;同时进行中段尿培养.结果 荧光原位杂交法检测阳性的有9例,与中段尿培养比较,其敏感度为100.0%,特异度为99.2%,阳性预期值为90.0%,阴性预期值为100.0%.结论 荧光原位杂交能快速检测尿液中的金黄色葡萄球菌,对金黄色葡萄球菌所致的尿路感染进行快速诊断.%Objective The rapid detection of Staphylococcus aureus in urine by fluorescence in situ hybridization (FISH)method. Screening urinary tract infection (UTIs) caused by Staphylococcus aureus. Methods Probes that were specific for Staphylococcus aureus were designed based on the conserved 16 s RNA sequences. Detected a total of 132 urine samples using FISH method. And all of tbese samples tested via traditional culturing techniques. Results 9 of these samples tested positive for a UTI via FISH analysis. Compared to conventional methods of bacterial identification,the FISH method had a sensitivity of 100.0% ,specificity of 99. 2%,positive expected value of 90. 0% and negative expected value of 100. 0%. Conclusion FISH had the potential to become an extremely useful diagnostic tool for UTIs because it has a quick turnaround time and high accuracy.

  8. Characterization of triacylglycerol composition of fish oils by using chromatographic techniques.

    Science.gov (United States)

    Solaesa, Ángela García; Bucio, Silvia Liliana; Sanz, María Teresa; Beltrán, Sagrario; Rebolleda, Sara

    2014-01-01

    Triacylglycerols (TAG) of two different refined fish oils from sardine and a mixture of tuna and sardine oil were separated by reverse phase high performance liquid chromatography (RP-HPLC) with a binary solvent gradient of acetone/acetonitrile. Different fractions were observed in the chromatogram and TAG species were tentatively identified by subsequent analysis of the fatty acid (FA) profile in each fraction by capillary Gas Chromatography (GC). Peak identities were assigned on the basis of a multiple linear regression analysis by using factors such as carbon number, number of double bonds, number of monounsaturated fatty acids (MUFA) and number of polyunsaturated fatty acids (PUFA) in the molecule as predictors for TAG retention time. A successful correlation was obtained between retention times and the equivalent carbon number (ECN) of triacylglycerols. Regiospecific analysis of fatty acids in the TAG has been conducted by ethanolysis of the fish oil by using an immobilized lipase. The subsequent separation of 2-monoacylglycerol (2-MAG) by TLC (thin layer chromatography) analysis showed that ethanolysis system is effective for analysis of FA composition at the 2-position in oils containing PUFA. Principal components analysis (PCA) has been also applied to establish correlations between the different fatty acids in the TAG.

  9. A hybrid conformal planning technique with solitary dynamic portal for postmastectomy radiotherapy with regional nodes

    Directory of Open Access Journals (Sweden)

    K Mohamathu Rafic

    2017-01-01

    Full Text Available Purpose: This study focuses on incorporation of a solitary dynamic portal (SDP in conformal planning for postmastectomy radiotherapy (PMRT with nodal regions with an intention to overcome the treatment planning limitations imposed by conventional techniques. Materials and Methods: Twenty-four patients who underwent surgical mastectomy followed by PMRT were included in this study. Initially, a treatment plan comprising tangential beams fitted to beam's-eye-view (BEV of chest wall (CW and a direct anterior field fitted to BEV of nodal region, both sharing a single isocenter was generated using Eclipse treatment planning system. Multiple field-in-fields with optimum beam weights (5% per field were added primarily from the medial tangent, fitted to BEV of entire target volume, and finally converted into a dynamic portal. Dosimetric analysis for the treatment plans and fluence verification for the dynamic portals were performed. Results and Discussion: Conformal plans with SDP showed excellent dose coverage (V95%>95%, higher degree of tumor dose conformity (≤1.25 and homogeneity (≤0.12 without compromising the organ at risk sparing for PMRT with nodal region. Treatment plans with SDP considerably reduced the lower isodose spread to the ipsilateral lung, heart, and healthy tissue without affecting the dose homogeneity. Further, gamma evaluation showed more than 96% pixel pass rate for standard 3%/3 mm dose difference and distance-to-agreement criteria. Moreover, this plan offers less probability of “geometrical miss” at the highly irregular CW with regional nodal radiotherapy. Conclusion: Hybrid conformal plans with SDP would facilitate improved dose distribution and reduced uncertainty in delivery and promises to be a suitable treatment option for complex postmastectomy CW with regional nodal irradiation.

  10. Passive hybrid technique for the vibration mitigation of systems of interconnected stays

    Science.gov (United States)

    Caracoglia, Luca; Jones, Nicholas P.

    2007-11-01

    The problem of stay oscillation mitigation in cable-stayed bridges, usually induced by wind or wind and rain, may require the introduction of passive devices, such as dampers on individual stays or the use of transverse restrainers (cross-ties). The damper performance is often affected by the geometrical constraints of the bridge deck that limit the installation of such devices to locations very close to the end of the cable. On the other hand, cross-ties are generally incapable of direct energy dissipation. Therefore, the authors have proposed and analyzed a hybrid passive system in which the advantages of both techniques are applied to the oscillation mitigation of complex interconnected systems with multiple external dampers at the deck level, in correspondence with the cross-tie lines. This paper summarizes the relevant findings of a research program involving the authors' efforts focused on the in-plane free-vibration analysis of stay-cable systems. This research is also based upon some recent results associated with the analytical solution of a taut-cable with two attached viscous dampers. These findings are initially extended to a simplified network with reduced number of connectors and one damper, for which the derivation of analytical solution is still possible. Subsequently, an existing multistay multidamped arrangement on a real bridge is considered, in which a fully numerical approach is required. The modal behavior is compared to the simplified examples, also enabling the interpretation of the results in the context of more general guidelines for potential future application.

  11. Prediction of monthly regional groundwater levels through hybrid soft-computing techniques

    Science.gov (United States)

    Chang, Fi-John; Chang, Li-Chiu; Huang, Chien-Wei; Kao, I.-Feng

    2016-10-01

    Groundwater systems are intrinsically heterogeneous with dynamic temporal-spatial patterns, which cause great difficulty in quantifying their complex processes, while reliable predictions of regional groundwater levels are commonly needed for managing water resources to ensure proper service of water demands within a region. In this study, we proposed a novel and flexible soft-computing technique that could effectively extract the complex high-dimensional input-output patterns of basin-wide groundwater-aquifer systems in an adaptive manner. The soft-computing models combined the Self Organized Map (SOM) and the Nonlinear Autoregressive with Exogenous Inputs (NARX) network for predicting monthly regional groundwater levels based on hydrologic forcing data. The SOM could effectively classify the temporal-spatial patterns of regional groundwater levels, the NARX could accurately predict the mean of regional groundwater levels for adjusting the selected SOM, the Kriging was used to interpolate the predictions of the adjusted SOM into finer grids of locations, and consequently the prediction of a monthly regional groundwater level map could be obtained. The Zhuoshui River basin in Taiwan was the study case, and its monthly data sets collected from 203 groundwater stations, 32 rainfall stations and 6 flow stations during 2000 and 2013 were used for modelling purpose. The results demonstrated that the hybrid SOM-NARX model could reliably and suitably predict monthly basin-wide groundwater levels with high correlations (R2 > 0.9 in both training and testing cases). The proposed methodology presents a milestone in modelling regional environmental issues and offers an insightful and promising way to predict monthly basin-wide groundwater levels, which is beneficial to authorities for sustainable water resources management.

  12. Effect of different binders and encapsulation techniques in the structure and functional properties of microdiets for fish larvae

    Directory of Open Access Journals (Sweden)

    Ana Grenha

    2014-05-01

    Full Text Available The improvement of micro diets, for fish larvae, has been a major challenge in past years; however some problems remain without a resolution. Besides its tremendous growth rates, at early developmental stages, fish larvae present an immature digestive system and a progressive metamorphic process that suggest special nutritional requirements. It is therefore essential to provide fish larvae with easy and/or quickly metabolized protein sources, like protein hydrolisates, high bioavailable forms of vitamins and trace minerals. The low molecular weight of such compounds, in association to the high surface area of the micro-particles, makes them highly soluble and prone to water leaching losses. These nutrient losses, not only makes these nutrients unavailable to the larvae, but originate an important increase of organic matter in the rearing tanks, leading to detrimental water quality criteria. The development of high-performing micro-particulate diets for fish larvae requires a delicate balance between an adequate quantitative and qualitative nutritional supply and good water stability of the feeds, without compromising the palatability and the digestive breakdown. A series of binders, protein-based (e.g. fish gelatin, wheat gluten, spray-dried plasma and carbohydrate-based (gums, alginates, pectins, starches, dextrin, polyols were incorporated at graded levels (1, 2.5 and 5% in a standard formulation for seabream larvae. Diets were manufactured by a proprietary low-shear extrusion process and afterwards processed to obtain a 200-400 micron fraction. All diets were analyzed for the following criteria: water leaching of nitrogenous compounds, turbidimetry and particle water stability. An additionally approach relied on the use of various encapsulation techniques (spray-drying, fluid-bed drying and vacuum coating to incorporate trace minerals (Zn and Mn and amino acids (taurine, lysine and methionine. Technological results show that the beneficial

  13. Hybridization leads to sensory repertoire expansion in a gynogenetic fish, the Amazon molly (poecilia formosa): a test of the hybrid-sensory expansion hypothesis.

    Science.gov (United States)

    Sandkam, Benjamin A; Joy, Jeffrey B; Watson, Corey T; Gonzalez-Bendiksen, Pablo; Gabor, Caitlin R; Breden, Felix

    2013-01-01

    Expansions in sensory systems usually require processes such as gene duplication and divergence, and thus evolve slowly. We evaluate a novel mechanism leading to rapid sensory repertoire expansion: hybrid-sensory expansion (HSE). HSE occurs when two species with differently tuned sensory systems form a hybrid, bringing together alleles from each of the parental species. In one generation, a sensory repertoire is created that is the sum of the variance between parental species. The Amazon molly presents a unique opportunity to test the HSE hypothesis in a "frozen" hybrid. We compared opsin sequences of the Amazon molly, Poecilia formosa, to those of the parental species. Both parental species are homozygous at the RH2-1 locus and each of the four long wavelength sensitive loci, while P. formosa possess two different alleles at these loci; one matching each parental allele. Gene expression analysis showed P. formosa use the expanded opsin repertoire that was the result of HSE. Additionally, behavioral tests revealed P. formosa respond to colored stimuli in a manner similar or intermediate to the parental species P. mexicana and P. latipinna. Together these results strongly support the HSE hypothesis. Hybrid-sensory repertoire expansion is likely important in other hybrid species and in other sensory systems.

  14. A review of the hybrid techniques for the fabrication of hard magnetic microactuators based on bonded magnetic powders

    Science.gov (United States)

    Pallapa, M.; Yeow, J. T. W.

    2015-02-01

    Polymer composites based on permanent magnetic bonded powders exhibit immense potential for applications in microactuators and sensors with magnetic performances comparable to their fully dense counterparts. While fabrication and integration of magnetic devices based on bonded magnetic powders is challenging via conventional deposition and electrochemical growth techniques, hybrid fabrication offers a promising alternative. This paper presents the evolution of permanent magnetic materials into bonded magnetic powders, the magnetic performance figures of merit of permanent magnetic materials significant for the design and manufacture of polymer based sensors and actuators. A review of the hybrid fabrication techniques such as replica molding, squeegee coating, spin casting etc are reported. Critical factors affecting the fabrication of polymer magnetic composites such as filler particle size and effect of magnetic field during fabrication are discussed. Prior art based on polymer magnetic composites for the fabrication of hard magnetic films and hard magnetic actuators are presented.

  15. Mixed potential integral equation technique for hybrid microstrip-slotline multilayered circuits using a mixed rectangular-triangular mesh

    Science.gov (United States)

    Sercu, Jeannick; Fache, Niels; Libbrecht, Frank; Lagasse, Paul

    1995-05-01

    In this paper, a mixed potential integral equation (MPIE) formulation for hybrid microstrip-slotline multilayered circuits is presented. This integral equation is solved with the method of moments (MoM) in combination with Galerkin's method. The vector-valued rooftop functions defined over a mixed rectangular-triangular mesh are used to model the electric and magnetic currents on the microstrip and slotline structures. An efficient calculation technique for the quadruple interaction integrals between two cells in the system matrix equation is presented. Two examples of hybrid microstrip-slotline circuits are discussed. The first example compares the simulation results for a microstrip-slotline transition with measured data. The second example illustrates the use of the simulation technique in the design process of a broadband slot-coupled microstrip line transition.

  16. A 3D hybrid grid generation technique and a multigrid/parallel algorithm based on anisotropic agglomeration approach

    Institute of Scientific and Technical Information of China (English)

    Zhang Laiping; Zhao Zhong; Chang Xinghua; He Xin

    2013-01-01

    A hybrid grid generation technique and a multigrid/parallel algorithm are presented in this paper for turbulence flow simulations over three-dimensional (3D) complex geometries.The hybrid grid generation technique is based on an agglomeration method of anisotropic tetrahedrons.Firstly,the complex computational domain is covered by pure tetrahedral grids,in which anisotropic tetrahedrons are adopted to discrete the boundary layer and isotropic tetrahedrons in the outer field.Then,the anisotropic tetrahedrons in the boundary layer are agglomerated to generate prismatic grids.The agglomeration method can improve the grid quality in boundary layer and reduce the grid quantity to enhance the numerical accuracy and efficiency.In order to accelerate the convergence history,a multigrid/parallel algorithm is developed also based on anisotropic agglomeration approach.The numerical results demonstrate the excellent accelerating capability of this multigrid method.

  17. Staging of recurrent and advanced lung cancer with 18F-FDG PET in a coincidence technique (hybrid PET).

    Science.gov (United States)

    Schmid, R A; Hautmann, H; Poellinger, B; Kellner, W; Moisseev, A; Brinkbaeumer, K; Weiss, M; Hahn, K; Dresel, S

    2003-01-01

    The aim of this study was to evaluate [18F]fluorodeoxyglucose ( F-FDG) imaging of recurrent or inoperable lung cancer using a hybrid positron emission tomography (PET) device of the third generation. Examinations were compared with the results of conventional staging. Thirty-six patients suffering from recurrent or primarily inoperable lung cancer (29 men, seven women; age 64.8+/-12.0 years) were examined using hybrid PET (Marconi Axis gamma-PET ) 60 min after injection of 370 MBq F-FDG. The data obtained were reconstructed iteratively. All patients received a computed tomography (CT) scan using either the spiral or multislice technique. All lesions suspicious for primary or recurrent tumour were verified by biopsy; mediastinal lymph nodes were considered as malignant, when positive histology or a small axis diameter of greater than 1 cm measured with CT in addition to progression of clinical course was found. Distant metastases were diagnosed by CT and bone scintigraphy. Using hybrid PET all lesions showed a focally elevated glucose metabolism. Lymph node involvement of the ipsilateral peribronchial and hilar station (N1) was identified in 24/26 cases (92%), in 26/29 cases (90%) of ipsilateral central manifestation (N2) and in 11/13 (85%) cases of central contralateral or supraclavicular lymphatic infestation (N3). Pulmonary spread in hybrid PET was found in 4/8 cases (50%), whereas mainly lung metastases with a diameter of 1.5 cm and smaller were missed. Pleural involvement diagnosed by CT was verified in 4/5 patients. All four patients with bony metastases in conventional staging also presented with positive findings in hybrid PET (8/9 lesions). Concordance with conventional staging was found in 28/36 of patients (78%). In 4/36 patients (11%) unknown sites of tumour were detected leading to therapeutic consequences in three patients after radiological confirmation. Hybrid PET would have led to an understaging in four cases (11%), resulting theoretically in

  18. Simultaneous 16S and 18S rRNA fluorescence in situ hybridization (FISH) on LR White sections demonstrated in Vestimentifera (Siboglinidae) tubeworms.

    Science.gov (United States)

    Schimak, Mario P; Toenshoff, Elena R; Bright, Monika

    2012-02-01

    Traditional morphological identification of invertebrate marine species is limited in early life history stages for many taxa. In this study, we demonstrate, by example of Vestimentiferan tubeworms (Siboglinidae, Polychaeta), that the simultaneous fluorescence in situ hybridization (FISH) of both eukaryotic host and bacterial symbiont cells is possible on a single semi-thin (1 μm) section. This allows the identification of host specimens to species level as well as offering visualization of bacteria distributed within the host tissue. Previously published 18S rRNA host-specific oligonucleotide probes for Riftia pachyptila, Tevnia jerichonana and a newly designed Oasisia alvinae probe, as well as a 16S rRNA probe targeting symbionts found in all host species, were applied. A number of standard fixation and hybridization parameters were tested and optimized for the best possible signal intensity and cellular resolution. Ethanol conserved samples embedded in LR White low viscosity resin yielded the best results with regard to both signal intensity and resolution. We show that extended storage times of specimens does not affect the quality of signals attained by FISH and use our protocol to identify morphologically unidentifiable tubeworm individuals from a small data set, conforming to previous findings in succession studies of the Siboglinidae family.

  19. Validation of microsatellite multiplexes for parentage analysis and species discrimination in two hybridizing species of coral reef fish (Plectropomus spp., Serranidae).

    Science.gov (United States)

    Harrison, Hugo B; Feldheim, Kevin A; Jones, Geoffrey P; Ma, Kayan; Mansour, Hicham; Perumal, Sadhasivam; Williamson, David H; Berumen, Michael L

    2014-06-01

    Microsatellites are often considered ideal markers to investigate ecological processes in animal populations. They are regularly used as genetic barcodes to identify species, individuals, and infer familial relationships. However, such applications are highly sensitive the number and diversity of microsatellite markers, which are also prone to error. Here, we propose a novel framework to assess the suitability of microsatellite datasets for parentage analysis and species discrimination in two closely related species of coral reef fish, Plectropomus leopardus and P. maculatus (Serranidae). Coral trout are important fisheries species throughout the Indo-Pacific region and have been shown to hybridize in parts of the Great Barrier Reef, Australia. We first describe the development of 25 microsatellite loci and their integration to three multiplex PCRs that co-amplify in both species. Using simulations, we demonstrate that the complete suite of markers provides appropriate power to discriminate between species, detect hybrid individuals, and resolve parent-offspring relationships in natural populations, with over 99.6% accuracy in parent-offspring assignments. The markers were also tested on seven additional species within the Plectropomus genus with polymorphism in 28-96% of loci. The multiplex PCRs developed here provide a reliable and cost-effective strategy to investigate evolutionary and ecological dynamics and will be broadly applicable in studies of wild populations and aquaculture brood stocks for these closely related fish species.

  20. Validation of microsatellite multiplexes for parentage analysis and species discrimination in two hybridizing species of coral reef fish (Plectropomus spp., Serranidae)

    KAUST Repository

    Harrison, H.B.

    2014-04-24

    Microsatellites are often considered ideal markers to investigate ecological processes in animal populations. They are regularly used as genetic barcodes to identify species, individuals, and infer familial relationships. However, such applications are highly sensitive the number and diversity of microsatellite markers, which are also prone to error. Here, we propose a novel framework to assess the suitability of microsatellite datasets for parentage analysis and species discrimination in two closely related species of coral reef fish, Plectropomus leopardus and P. maculatus (Serranidae). Coral trout are important fisheries species throughout the Indo-Pacific region and have been shown to hybridize in parts of the Great Barrier Reef, Australia. We first describe the development of 25 microsatellite loci and their integration to three multiplex PCRs that co-amplify in both species. Using simulations, we demonstrate that the complete suite of markers provides appropriate power to discriminate between species, detect hybrid individuals, and resolve parent-offspring relationships in natural populations, with over 99.6% accuracy in parent-offspring assignments. The markers were also tested on seven additional species within the Plectropomus genus with polymorphism in 28-96% of loci. The multiplex PCRs developed here provide a reliable and cost-effective strategy to investigate evolutionary and ecological dynamics and will be broadly applicable in studies of wild populations and aquaculture brood stocks for these closely related fish species. 2014 The Authors. Ecology and Evolution published by John Wiley & Sons Ltd.

  1. Emulsification technique affects oxidative stability of fish oil-in-water emulsions

    DEFF Research Database (Denmark)

    Horn, Anna Frisenfeldt; Nielsen, Nina Skall; Jensen, Louise Helene Søgaard

    In oil-in-water emulsions, lipid oxidation is expected to be initiated at the oil-water interface. The properties of the emulsifier used, and the structure at the interface is therefore expected to be of great importance for lipid oxidation in emulsions. Previous studies have shown that e...... of this study was to compare lipid oxidation in 10% fish oil-in-water emulsions prepared by two different kinds of high pressure homogenizers i.e. a microfluidizer and a two valve high pressure homogenizer. Emulsions were made with equal droplet sizes, and with either 1% sodium caseinate or 1% whey protein.......g. homogenization pressure can affect how proteins locate themselves at the interface of an emulsion. The hypothesis is therefore that emulsions produced with different emulsification equipments differ in their oxidative stability due to differences in the behaviour of the proteins at the interface. The aim...

  2. Emulsification technique affects oxidative stability of fish oil-in-water emulsion

    DEFF Research Database (Denmark)

    Horn, Anna Frisenfeldt; Nielsen, Nina Skall; Jensen, Louise Helene Søgaard

    In oil-in-water emulsions, lipid oxidation is expected to be initiated at the oil-water interface. The properties of the emulsifier used, and the structure at the interface is therefore expected to be of great importance for lipid oxidation in emulsions. Previous studies have shown that e...... of this study was therefore to compare lipid oxidation in 10% fish oil-in-water emulsions prepared by two different kinds of high pressure homogenizers i.e. a microfluidizer and a two valve high pressure homogenizer. Emulsions were made with equal droplet sizes, and with either 1% sodium caseinate or 1% whey.......g. homogenization pressure can affect how proteins locate themselves at the interface of an emulsion. The hypothesis is therefore that emulsions produced with different emulsification equipments differ in their oxidative stability due to differences in the behaviour of the proteins at the interface. The aim...

  3. Genome analysis of 7 Kengyilia (Triticeae Poaceae) species with FISH and GISH

    Science.gov (United States)

    Genome composition of and genetic relationships among seven Kengyilia species were assessed using a technique of sequential FISH (fluorescence in situ hybridization) and GISH (genomic in situ hybridization). Five of these 7 species, K. kokonorica, K. rigidula, K. hirsula, K. grandiglumis, and K. th...

  4. POWER OPTIMIZED DATAPATH UNITS OF HYBRID EMBEDDED CORE ARCHITECTURE USING CLOCK GATING TECHNIQUE

    National Research Council Canada - National Science Library

    T.Subhashini; M.Kamaraju

    2015-01-01

    ...% of the total power dissipation. The main goal of this work is to implement a prototype power optimized datapath unit and ALU of Hybrid Embedded Controller Architecture targeted on to the FPGA chip and analyze the power consumption...

  5. Fish meal quality evaluated by chemical analysis and feed microscopy techniques

    Directory of Open Access Journals (Sweden)

    Anuchit Arthan

    2005-02-01

    Full Text Available Quality of 20 fishmeal samples from the Southern Provinces produced by different raw materials and processing method were evaluated using both chemical and feed microscopy methods. Samples were collected from fishmeal plants, shrimp feed mills and local feedstuff store. One sample using sardine as a raw material was produced in the laboratory by lyophilization as a control fishmeal. Five samples having protein contents over 65% were grouped into shrimp grade including the control fishmeal which had the maximum protein content of 74.09%. Eight samples were grouped into Grade 1 including the imported fishmeal from Chile. The rest of the samples which were mostly produced from trash fish fell into Grade 2 with low protein and high ash content. Chemical analyses of salt content, total volatile base nitrogen (TVN, ammonia nitrogen, histamine content, free fatty acid (FFA, thiobarbituric acid number (TBA and peroxide value showed the fishmeal from feedstuff store was the most deteriorated sample. This high level of deterioration might be due to low quality raw materials and a long storage time. Among the shrimp grade samples, the most denatured protein was found in Pattani 1 with high level of TVN, ammonia nitrogen and histamine. Moreover, the high levels of TVN were detected in Phangnga 1 (grade 1 and Trang 2 (grade 2. Lipid damage was associated with high fat contents in fish meal which was detected in every group of the samples. The results from feed microscopic examination were in line with those of the chemical analyses. The most deteriorated sample from feedstuff store showed the highest decomposition level and was the only sample that non protein nitrogen was detected. For feather meal adulteration test, seven samples were positive with the highest level in unidentified sample and trace amount in other samples including 2 shrimp grade samples. To confirm feather meal adulteration, protein digestibility test should be performed for these samples.

  6. Fish recognition based on the combination between robust feature selection, image segmentation and geometrical parameter techniques using Artificial Neural Network and Decision Tree

    CERN Document Server

    Alsmadi, Mutasem Khalil Sari; Noah, Shahrul Azman; Almarashdah, Ibrahim

    2009-01-01

    We presents in this paper a novel fish classification methodology based on a combination between robust feature selection, image segmentation and geometrical parameter techniques using Artificial Neural Network and Decision Tree. Unlike existing works for fish classification, which propose descriptors and do not analyze their individual impacts in the whole classification task and do not make the combination between the feature selection, image segmentation and geometrical parameter, we propose a general set of features extraction using robust feature selection, image segmentation and geometrical parameter and their correspondent weights that should be used as a priori information by the classifier. In this sense, instead of studying techniques for improving the classifiers structure itself, we consider it as a black box and focus our research in the determination of which input information must bring a robust fish discrimination.The main contribution of this paper is enhancement recognize and classify fishes...

  7. RAPD Technique Used to Determine the Purity of Hybrid Hot Pepper Seeds

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    Two hybrid hot pepper varieties Xiangyan 5 and Xiangyan 10, and their parents were analyzed the polymerase chain reaction with MJ /PT 200 Peltrier Themal Cycler and DS 800 White-ultravilot Transilluminator to set up a RAPD system adaptable to the purity determination of the hybrid seeds. Among the 39 random primers, 2 and 4 primers were found to be used effectively in Xiangyan 5 and Xiangyan 10 respectively.

  8. Optimisation Sizing of Hybrid Wind-Diesel Systems using Linear Programming Technique

    OpenAIRE

    Gan, Leong Kit; Shek, Jonathan; Mueller, Markus

    2014-01-01

    Despite the great potential of hybrid wind-diesel system in supplying energy to remote or island communities, sizing the system components have been a challenging problem for many project managers due to the reliance on various factors. This work considers utilising a fixed speed wind turbine (induction generator) in the hybrid system. It requires energy for start-up operation and this work takes into account for sizing the battery storage. In addition, the trade-off between the number of bat...

  9. Application and analysis of LNG cold energy for hybrid fishing vessel%混合动力渔船LNG冷量的应用分析

    Institute of Scientific and Technical Information of China (English)

    魏鹏威; 张建一; 詹鋆

    2012-01-01

    液化天然气(LNC)是清洁能源,同时又蕴含大量冷量,我国已经提出渔船推广使用LNG作为动力燃料.目前,渔船制冷能耗占全船能耗30%-40%.为了确定混合动力渔船中LNG的应用优势,本文结合实际生产中的50m型围网渔船进行了计算分析.结果显示,在该型渔船上采用柴油/天然气混合动力,替油率80%时每年可节省燃料费用61万元.将LNG冷量用于冷海水系统,每天可制取0℃冷海水10.4t,减少冷海水制冷系统工作时长1.6h,部分冷量用于系统的过冷循环,可使系统COP提高5.2%.分析表明,利用混合动力渔船的LNG冷能可显著节能,降低营运成本.%Liquified Natural Gas (LNG) is a clean energy with a lot of cold energy.Its use as engine fuel for fishing vessel is promoted currently in China.The energy consumption of refrigeration system in fishing vessels has accounted for 30% to 40% in total energy consumption.In order to determine the advantage of LNG applications in hybrid fishing vessel,some calculation and analysis are conducted based on 50 m model purse seine vessel.The results show that 610 thousands yuan can be saved annually if 80% of diesel oil is replaced by LNG on this type of fishing vessels.Meanwhile,10.4 t cold seawater in 0℃ can be produced and 1.6 hour of the system's working time can be reduced each day by use of the LNG cold energy.The COP can be improved by 5.2% when the surplus LNG cold energy is used for subcooled cycle of the refrigeration system.Energy cost can be significantly reduced by use of LNG cold energy in the hybrid fishing vessels based on the analysis.

  10. Fluorescence In Situ Hybridization (FISH-Based Karyotyping Reveals Rapid Evolution of Centromeric and Subtelomeric Repeats in Common Bean (Phaseolus vulgaris and Relatives

    Directory of Open Access Journals (Sweden)

    Aiko Iwata-Otsubo

    2016-04-01

    Full Text Available Fluorescence in situ hybridization (FISH-based karyotyping is a powerful cytogenetics tool to study chromosome organization, behavior, and chromosome evolution. Here, we developed a FISH-based karyotyping system using a probe mixture comprised of centromeric and subtelomeric satellite repeats, 5S rDNA, and chromosome-specific BAC clones in common bean, which enables one to unambiguously distinguish all 11 chromosome pairs. Furthermore, we applied the karyotyping system to several wild relatives and landraces of common bean from two distinct gene pools, as well as other related Phaseolus species, to investigate repeat evolution in the genus Phaseolus. Comparison of karyotype maps within common bean indicates that chromosomal distribution of the centromeric and subtelomeric satellite repeats is stable, whereas the copy number of the repeats was variable, indicating rapid amplification/reduction of the repeats in specific genomic regions. In Phaseolus species that diverged approximately 2–4 million yr ago, copy numbers of centromeric repeats were largely reduced or diverged, and chromosomal distributions have changed, suggesting rapid evolution of centromeric repeats. We also detected variation in the distribution pattern of subtelomeric repeats in Phaseolus species. The FISH-based karyotyping system revealed that satellite repeats are actively and rapidly evolving, forming genomic features unique to individual common bean accessions and Phaseolus species.

  11. Resolving colocalization of bacteria and metal(loid)s on plant root surfaces by combining fluorescence in situ hybridization (FISH) with multiple-energy micro-focused X-ray fluorescence (ME μXRF).

    Science.gov (United States)

    Honeker, Linnea K; Root, Robert A; Chorover, Jon; Maier, Raina M

    2016-12-01

    Metal(loid)-contamination of the environment due to anthropogenic activities is a global problem. Understanding the fate of contaminants requires elucidation of biotic and abiotic factors that influence metal(loid) speciation from molecular to field scales. Improved methods are needed to assess micro-scale processes, such as those occurring at biogeochemical interfaces between plant tissues, microbial cells, and metal(loid)s. Here we present an advanced method that combines fluorescence in situ hybridization (FISH) with synchrotron-based multiple-energy micro-focused X-ray fluorescence microprobe imaging (ME μXRF) to examine colocalization of bacteria and metal(loid)s on root surfaces of plants used to phytostabilize metalliferous mine tailings. Bacteria were visualized on a small root section using SytoBC nucleic acid stain and FISH probes targeting the domain Bacteria and a specific group (Alphaproteobacteria, Gammaproteobacteria, or Actinobacteria). The same root region was then analyzed for elemental distribution and metal(loid) speciation of As and Fe using ME μXRF. The FISH and ME μXRF images were aligned using ImageJ software to correlate microbiological and geochemical results. Results from quantitative analysis of colocalization show a significantly higher fraction of As colocalized with Fe-oxide plaques on the root surfaces (fraction of overlap 0.49±0.19) than to bacteria (0.072±0.052) (pbacteria that colocalized with metal(loid)s, Actinobacteria, known for their metal tolerance, had a higher correlation with both As and Fe than Alphaproteobacteria or Gammaproteobacteria. This method demonstrates how coupling these micro-techniques can expand our understanding of micro-scale interactions between roots, metal(loid)s and microbes, information that should lead to improved mechanistic models of metal(loid) speciation and fate. Copyright © 2016 Elsevier B.V. All rights reserved.

  12. Electrofishing as a sampling technique for coastal stream fish populations and communities in the Southeast of Brazil.

    Science.gov (United States)

    Mazzoni; Fenerich-Verani; Caramaschi

    2000-05-01

    Electrofishing adequacy was tested as a technique to obtain quantitative data of coastal stream fish populations and communities in the Southeast of Brazil. Seven field trips, between July/94 and July/95, were done in 5 localities of the Ubatiba fluvial system (Maricá, RJ). Seventeen species, among the 22 collected, had their numbers estimated through the Zipping method, the model used to test the sampling methodology. At each field trip, three removals with electrofishing were done in each locality and, according to the number of obtained species at each locality/field trip, we analysed 315 cases. Nineteen cases, among 315, showed failure condition. Estimates were significant (p catchability and the estimated number of individuals and/or environmental characteristics. High values for sampling efficiency (> 85%) were found for all estimates. An experimental analyses were done for one locality and, the comparison between the estimates for 3 and 6 successive removals showed a mean error and a standard deviation of 5.5% and 2.1% respectively. Therefore, it can be concluded that electrofishing was an efficient method for quantitative data analysis of fish populations and communities in the Ubatiba fluvial system.

  13. PET/MRI: a novel hybrid imaging technique. Major clinical indications and preliminary experience in Brazil.

    Science.gov (United States)

    Vitor, Taise; Martins, Karine Minaif; Ionescu, Tudor Mihai; Cunha, Marcelo Livorsi da; Baroni, Ronaldo Hueb; Garcia, Marcio Ricardo Taveira; Wagner, Jairo; Campos, Guilherme de Carvalho; Nogueira, Solange Amorim; Guerra, Elaine Gonçalves; Amaro, Edson

    2017-01-01

    In recent years, medical imaging with hybrid techniques has widely accepted and employed in clinical routine. PET/MRI offers significant advantages, including excellent contrast and resolution and reduced ionizing radiation, as compared to well-established PET/CT. Therefore, PET/MRI is a promising modality for oncologic imaging of some regions, such as brain, head and neck, liver and pelvis. This article set out to analyze clinical conditions that could benefit from PET/MRI imaging based on our caseload. The potential of PET/MRI to become the imaging modality of choice for assessment of neurologic and oncologic conditions associated with soft tissues is highlighted. Clinical aspects of PET/MRI and its application to clinical cases are illustrated with examples extracted from the authors' preliminary experience. RESUMO Nos últimos anos, imagens médicas com tecnologias híbridas tornaram-se amplamente aceitas e utilizadas na prática clínica. O PET/RM possui vantagens importantes, incluindo excelentes contrastes e resolução, e menor radiação ionizante, em comparação ao PET/TC. Por isto, é uma modalidade promissora para exames de imagem de pacientes oncológicos, para avaliar o cérebro, cabeça e pescoço, o fígado e a pelve. O objetivo deste artigo foi analisar as situações clínicas que se beneficiariam de exames de PET/RM a partir de uma casuística. Destacamos o potencial desta técnica se tornar o método de imagem de escolha para doenças neurológicas e oncológicas que envolvam partes moles. Os aspectos clínicos de PET/RM e sua aplicação aos casos clínicos são ilustrados com exemplos da experiência inicial dos autores.

  14. Novel fabrication technique of hybrid structure lens array for 3D images

    Science.gov (United States)

    Lee, Junsik; Kim, Junoh; Kim, Cheoljoong; Shin, Dooseub; Koo, Gyohyun; Won, Yong Hyub

    2016-03-01

    Tunable liquid lens arrays can produce three dimensional images by using electrowetting principle that alters surface tensions by applying voltage. This method has advantages of fast response time and low power consumption. However, it is challenging to fabricate a high fill factor liquid lens array and operate three dimensional images which demand high diopter. This study describes a hybrid structure lens array which has not only a liquid lens array but a solid lens array. A concave-shape lens array is unavoidable when using only the liquid lens array and some voltages are needed to make the lens flat. By placing the solid lens array on the liquid lens array, initial diopter can be positive. To fabricate the hybrid structure lens array, a conventional lithographic process in semiconductor manufacturing is needed. A negative photoresist SU-8 was used as chamber master molds. PDMS and UV adhesive replica molding are done sequentially. Two immiscible liquids, DI water and dodecane, are injected in the fabricated chamber, followed by sealing. The fabricated structure has a 20 by 20 pattern of cylindrical shaped circle array and the aperture size of each lens is 1mm. The thickness of the overall hybrid structure is about 2.8mm. Hybrid structure lens array has many advantages. Solid lens array has almost 100% fill factor and allow high efficiency. Diopter can be increased by more than 200 and negative diopter can be shifted to the positive region. This experiment showed several properties of the hybrid structure and demonstrated its superiority.

  15. FISH and FICTION to detect chromosomal aberrations in lymphomas.

    Science.gov (United States)

    Giefing, Maciej; Siebert, Reiner

    2013-01-01

    Fluorescence In Situ Hybridization (FISH) is a powerful and robust technique allowing the visualization of target sequences like genes in interphase nuclei. It is widely used in routine diagnostics to identify cancer specific aberrations including lymphoma associated translocations or gene copy number changes in single tumor cells. By combining FISH with immunophenotyping-a technique called Fluorescence Immunophenotyping and Interphase Cytogenetic as a Tool for Investigation Of Neoplasia (FICTION)-it is moreover possible to identify a cell population of interest. Here we describe standard protocols for FISH and FICTION as used in our laboratory in diagnosis and research.

  16. An identification in fish of the genus Puntius Hamilton 1822 (Cypriniformes: Cyprinidae) of some wetlands in northeast Thailand with the use of random amplified polymorphic DNA technique.

    Science.gov (United States)

    Champasri, T; Rapley, R; Duangjinda, M; Suksri, A

    2008-02-15

    The experiment was carried out during the 2003 to 2006 at the Department of Fisheries, Khon Kaen University, Khon Kaen, Thailand in collaboration with the Department of Biosciences, the University of Hertfordshire, College Land, Hatfield, Herts, UK. Molecular RAPD technique was used for the determinations of DNA patterns of the fish genus Puntius Hamilton 1822. The fish samples of 1,500 individual fish were collected from fifteen wetlands in Northeast Thailand and they were used for DNA extraction. Before the experiment was carried out the fish samples were morphologically identified and it was found that the collected fish consisted of 9 species i.e., Puntius altus, P. aurotaeniatus, P. binotatus, P. gonionotus, (e) P. leiacanthus, P. orphoides, P. partipentazona, P. schwanenfeldi and P. wetmorei. Genomic DNAs were extracted from 5 mg of muscle tissues (skeleton muscles) with the use of PUREGENE DNA Isolation Kit for Laboratory Use, Gentra Systems, USA. Eighty decamer primers from four kits were subjected to a preliminary test. It was found that only 10 decamer primers were most suited for this PCR amplification. The results showed that genetic distant values being established among and between pairs of the fishes of the 9 fish species ranged from 0.191 to 0.456 for a pair between Puntius gonionotus and Puntius altus and a pair between Puntius schwanenfeldi and Puntius leiacanthus, respectively. Similarity coefficient values within the 9 fish species ranged from 0.109 to 0.231. The results on a Dendrogram of clusters showed that there were 5 minor groups of the 9 fish species but the 9 species could not be split or shifted into other genera of the fish due to small differences found within the values of similarity coefficients.

  17. HybridSPE: A novel technique to reduce phospholipid-based matrix effect in LC-ESI-MS Bioanalysis.

    Science.gov (United States)

    Ahmad, Shafeeque; Kalra, Harsh; Gupta, Amit; Raut, Bharat; Hussain, Arshad; Rahman, Md Akhlaquer

    2012-10-01

    When complex biological materials are analyzed without an adequate sample preparation technique, MS signal and response undergo significant alteration and result in poor quantification and assay. This problem generally takes place due to the presence of several endogenous materials component in samples. One of the major causes of ion suppression in bioanalysis is the presence of phospholipids during LC-MS analysis. The phospholipid-based matrix effect was investigated with a commercially available electro spray ionization (ESI) source coupled with a triple quadrupole mass spectrometer. HybridSPE dramatically reduced the levels of residual phospholipids in biological samples, leading to significant reduction in matrix effects. This new procedure that combines the simplicity of precipitation with the selectivity of SPE allows obtaining much cleaner extracts than with conventional procedures. HybridSPE-precipitation procedure provides significant improvement in bioanalysis and a practical and fast way to ensure the avoidance of phospholipids-based matrix effects. The present review outlines the HybridSPE technique to minimize phospholipids-based matrix effects on LC-ESI-MS bioanalysis.

  18. HybridSPE: A novel technique to reduce phospholipid-based matrix effect in LC-ESI-MS Bioanalysis

    Directory of Open Access Journals (Sweden)

    Shafeeque Ahmad

    2012-01-01

    Full Text Available When complex biological materials are analyzed without an adequate sample preparation technique, MS signal and response undergo significant alteration and result in poor quantification and assay. This problem generally takes place due to the presence of several endogenous materials component in samples. One of the major causes of ion suppression in bioanalysis is the presence of phospholipids during LC-MS analysis. The phospholipid-based matrix effect was investigated with a commercially available electro spray ionization (ESI source coupled with a triple quadrupole mass spectrometer. HybridSPE dramatically reduced the levels of residual phospholipids in biological samples, leading to significant reduction in matrix effects. This new procedure that combines the simplicity of precipitation with the selectivity of SPE allows obtaining much cleaner extracts than with conventional procedures. HybridSPE-precipitation procedure provides significant improvement in bioanalysis and a practical and fast way to ensure the avoidance of phospholipids-based matrix effects. The present review outlines the HybridSPE technique to minimize phospholipids-based matrix effects on LC-ESI-MS bioanalysis.

  19. Hybrid approach to AAA: bilateral "banana" technique to preserve hypogastric artery in complex anatomy aorto-biiliac aneurysm.

    Science.gov (United States)

    Mosquera Arochena, N; Molina Herrero, F; Carbalho Fernandez, C; Rodriguez Feijoo, G; Fernandez Lebrato, R; Barrios Castro, A; Garcia Fernandez, I

    2011-01-01

    The surgical approach for hypogastric preservation in aorto-iliac aneurysm (AAA) open repair (OR) has been described and performed with different techniques but all of them represent a higher mortality and potencial complications to the procedure; this is even more critical in bilateral disease. Since the introduction of the first endograft, a continuous development has occurred, such as the stent graft with specific branch designed for preserving antegrade flow in the hypogastric artery. On highly angulated and tortuous iliac anatomies, the use of Sandwich-Graft technique, as described by Armando Lobato, represents a valid alternative to iliac branch. The hybrid approach could be a good treatment option in young patients with AAA affecting hypogastric arteries. We present the technical description and a case report of bilateral "banana" technique perfor- med with flexible covered stent (Viabahn(®) WL Gore) to preserve both hypogastric arteries combined with open repair in a 52 years old patient. Technical report and Results: A bilateral retrograde endograft was implanted from both external ilac arteries to hypo- gastric artery excluding bilateral common iliac aneurysms followed by an open repair to the AAA (aneurismectomy + aorto bifemoral by-pass) with good inmediate and short-midterm follow up (12 months) This hybrid technique could be a good approach to hypogastric preservation in low risk and young patients reducing potencial complications of hypogastric artery oclusion.

  20. Behavior of meiotic chromosomes in Pinus wallichiana, P. strobus and their hybrid and nrDNA localization in pollen mother cells of the hybrid by using FISH.

    Science.gov (United States)

    Deng, Hui-Sheng; Zhang, Da-Ming; Fu, Cheng-Xin; Hong, De-Yuan

    2008-03-01

    The complete process of meiosis was investigated in Pinus wallichiana, P. strobus and their artificial hybrid (F1) using microsporocytes. It is revealed that there were slightly lower chiasma frequency, lower ring bivalent frequency, lower meiotic index and distinctly higher frequency of aberrance (chromosomal bridges, fragments or micronuclei) in pollen mother cells (PMCs) of the hybrid (F1) than those of the parental species, which showed a certain degree of differentiation between homologous chromosomes of the two parents. However, relatively higher frequency of ring bivalents and higher meiotic index in all the three entities indicate the great stability of genomes of parental species, and the differentiation of genomes between the two parents must have been slight. Total nineteen signal loci of 18S rDNA were observed in nine bivalents of the hybrid (F1), among which one bivalent bears two loci, while the others have only one. It is suggested that distinct differentiation at genetic level existed in homologous chromosomes of the two parental species, whereas only slight differentiation at karyotypic and genomic levels take place between the parent species.

  1. Behavior of Meiotic Chromosomes in Pinus wallichiana, P. strobus and Their Hybrid and nrDNA Localization in Pollen Mother Cells of the Hybrid by Using FISH

    Institute of Scientific and Technical Information of China (English)

    Hui-Sheng Deng; Da-Ming Zhang; Cheng-Xin Fu; De-Yuan Hong

    2008-01-01

    The complete process of meiosis was investigated in Pinus wallichiana, P. strobus and their artificial hybrid (F1) using microsporocytes. It is revealed that there were slightly lower chiasma frequency, lower ring bivalent frequency, lower meiotic Index and distinctly higher frequency of aberrance (chromosomal bridges, fragments or micronuclei) in pollen mother cells (PMCs) of the hybrid (F1) than those of the parental species, which showed a certain degree of differentiation between homologous chromosomes of the two parents. However, relatively higher frequency of ring bivalents and higher meiotic index in all the three entities indicate the great stability of genomes of parental species, and the differentiation of genomes between the two parents must have been slight. Total nineteen signal loci of 18S rDNA were observed in nine bivalents of the hybrid (F1), among which one bivalent bears two loci, while the others have only one. It is suggested that distinct differentiation at genetic level existed in homologous chromosomes of the two parental species, whereas only slight differentiation at karyotypic and genomic levels take place between the parent species.

  2. N-doped ZnO films grown from hybrid target by the pulsed laser deposition technique

    Science.gov (United States)

    Martín-Tovar, E. A.; Chan y Díaz, E.; Acosta, M.; Castro-Rodríguez, R.; Iribarren, A.

    2016-10-01

    ZnO thin films were grown by the pulsed laser deposition technique on glass substrate using a hybrid target composed of ZnO powder embedded into a poly(ethyl cyanoacrylate) matrix. The resulting thin film presented ZnO wurtzite structure with very low stress and diffractogram very similar to that of the powder pattern. From comparing with ZnO thin films grown from traditional sintered target, it is suggested that the use of this hybrid target with a soft matrix led to ejection of ZnO clusters that conveniently disposed and adhered to substrate and previous deposited layers. Chemical measurements showed the presence of Zn-N bonds, besides Zn-O ones. Optical absorption profile confirmed the presence of low-polymerized zinc oxynitride molecular subunits, besides ZnO.

  3. Dielectric property determination of hybrid Al2O3-filled MWCNT buckypaper by the rectangular cavity perturbation technique

    Science.gov (United States)

    Miao, Hsin-Yuan; Liu, Jih-Hsin; Saravanan, L.; Tsao, Che-Wei; Pan, Jui-Wen

    2015-04-01

    This study investigated the complex dielectric permittivity of freestanding multiwalled carbon nanotube buckypaper (MWCNT-BP) and a synthesized hybrid alumina-filled buckypaper (Al2O3-BP) composite with different alumina loadings (5-30 wt%). The non-destructive microwave transmission technique for complex permittivity determination involving cavity perturbation was employed to characterize a set of Al2O3-BP sheets. This was done by filling a rectangular cavity resonator with a standard dielectric Teflon sample and then performing permittivity measurements for the buckypaper (BP) samples in the X-band frequency range (7-12 GHz). Field-emission scanning electron microscopy (FESEM) was used to analyze the morphology of the MWCNT-BP and the alumina-loaded BP composites. DC electrical resistivity measurements clearly demonstrated conductor-insulator transition. The effect of alumina loadings on the dielectric properties of the synthesized hybrid Al2O3-BP sheet is discussed.

  4. Hybrid models for hydrological forecasting: integration of data-driven and conceptual modelling techniques

    NARCIS (Netherlands)

    Corzo Perez, G.A.

    2009-01-01

    This book presents the investigation of different architectures of integrating hydrological knowledge and models with data-driven models for the purpose of hydrological flow forecasting. The models resulting from such integration are referred to as hybrid models. The book addresses the following top

  5. A hybrid method for flood simulation in small catchments combining hydrodynamic and hydrological techniques

    Science.gov (United States)

    Bellos, Vasilis; Tsakiris, George

    2016-09-01

    The study presents a new hybrid method for the simulation of flood events in small catchments. It combines a physically-based two-dimensional hydrodynamic model and the hydrological unit hydrograph theory. Unit hydrographs are derived using the FLOW-R2D model which is based on the full form of two-dimensional Shallow Water Equations, solved by a modified McCormack numerical scheme. The method is tested at a small catchment in a suburb of Athens-Greece for a storm event which occurred in February 2013. The catchment is divided into three friction zones and unit hydrographs of 15 and 30 min are produced. The infiltration process is simulated by the empirical Kostiakov equation and the Green-Ampt model. The results from the implementation of the proposed hybrid method are compared with recorded data at the hydrometric station at the outlet of the catchment and the results derived from the fully hydrodynamic model FLOW-R2D. It is concluded that for the case studied, the proposed hybrid method produces results close to those of the fully hydrodynamic simulation at substantially shorter computational time. This finding, if further verified in a variety of case studies, can be useful in devising effective hybrid tools for the two-dimensional flood simulations, which are lead to accurate and considerably faster results than those achieved by the fully hydrodynamic simulations.

  6. Hybrid models for hydrological forecasting: Integration of data-driven and conceptual modelling techniques

    NARCIS (Netherlands)

    Corzo Perez, G.A.

    2009-01-01

    This book presents the investigation of different architectures of integrating hydrological knowledge and models with data-driven models for the purpose of hydrological flow forecasting. The models resulting from such integration are referred to as hybrid models. The book addresses the following top

  7. Role of hybrid forecasting techniques for transportation planning of broiler meat under uncertain demand in thailand

    Directory of Open Access Journals (Sweden)

    Thoranin Sujjaviriyasup

    2014-12-01

    Full Text Available One of numerous problems experiencing in supply chain management is the demand. Most demands are appeared in terms of uncertainty. The broiler meat industry is inevitably encountering the same problem. In this research, hybrid forecasting model of ARIMA and Support Vector Machine (SVMs are developed to forecast broiler meat export. In addition, ARIMA, SVMs, and Moving Average (MA are chosen for comparing the forecasting efficiency. All the forecasting models are tested and validated using the data of Brazil’s export, Canada’s export, and Thailand’s export. The hybrid model provides accuracy of the forecasted values that are 98.71%, 97.50%, and 93.01%, respectively. In addition, the hybrid model presents the least error of all MAE, RMSE, and MAPE comparing with other forecasting models. As forecasted data are applied to transportation planning, the mean absolute percentage error (MAPE of optimal value of forecasted value and actual value is 14.53%. The hybrid forecasting model shows an ability to reduce risk of total cost of transportation when broiler meat export is forecasted by using MA(2, MA(3, ARIMA, and SVM are 50.59%, 60.18%, 68.01%, and 46.55%, respectively. The results indicate that the developed forecasting model is recommended to broiler meat industries’ supply chain decision.

  8. Hybrid and Model-Based Iterative Reconstruction Techniques for Pediatric CT

    NARCIS (Netherlands)

    den Harder, Annemarie M.; Willemink, Martin J.; Budde, Ricardo P. J.; Schilham, Arnold M. R.; Leiner, Tim; de Jong, Pim A.

    2015-01-01

    OBJECTIVE. Radiation exposure from CT examinations should be reduced to a minimum in children. Iterative reconstruction (IR) is a method to reduce image noise that can be used to improve CT image quality, thereby allowing radiation dose reduction. This article reviews the use of hybrid and model-bas

  9. Hybrid models for hydrological forecasting: integration of data-driven and conceptual modelling techniques

    NARCIS (Netherlands)

    Corzo Perez, G.A.

    2009-01-01

    This book presents the investigation of different architectures of integrating hydrological knowledge and models with data-driven models for the purpose of hydrological flow forecasting. The models resulting from such integration are referred to as hybrid models. The book addresses the following

  10. Hybrid models for hydrological forecasting: Integration of data-driven and conceptual modelling techniques

    NARCIS (Netherlands)

    Corzo Perez, G.A.

    2009-01-01

    This book presents the investigation of different architectures of integrating hydrological knowledge and models with data-driven models for the purpose of hydrological flow forecasting. The models resulting from such integration are referred to as hybrid models. The book addresses the following

  11. Biological evaluation of zirconia/PEG hybrid materials synthesized via sol-gel technique.

    Science.gov (United States)

    Catauro, M; Papale, F; Bollino, F; Gallicchio, M; Pacifico, S

    2014-07-01

    The objective of the following study has been the synthesis via sol-gel and the characterization of novel organic-inorganic hybrid materials to be used in biomedical field. The prepared materials consist of an inorganic zirconia matrix containing as organic component the polyethylene glycol (PEG), a water-soluble polymer used in medical and pharmaceutical fields. Various hybrids have been synthesized changing the molar ratio between the organic and inorganic parts. Fourier transform spectroscopy suggests that the structure of the interpenetrating network is realized by hydrogen bonds between the Zr-OH group in the sol-gel intermediate species and both the terminal alcoholic group and ethereal oxygen atoms in the repeating units of polymer The amorphous nature of the gels has been ascertained by X-ray diffraction analysis. The morphology observation has been carried out by using the Scanning Electron Microscope and has confirmed that the obtained materials are nanostructurated hybrids. The bioactivity of the synthesized system has been shown by the formation of a hydroxyapatite layer on the surface of samples soaked in a fluid simulating the human blood plasma. The potential biocompatibility of hybrids has been assessed as performing indirect MTT cytotoxicity assay towards 3T3 cell line at 24, 48, and 72 h exposure times. Copyright © 2014. Published by Elsevier B.V.

  12. Rapid high-throughput genotyping of HBV DNA using a modified hybridization-extension technique.

    Science.gov (United States)

    Bao, Han; Zhao, Wenliang; Ruan, Banjun; Wang, Qing; Zhao, Jinrong; Lei, Xiaoying; Wang, Weihua; Liu, Yonglan; Sun, Jianbing; Xiang, An; Guo, Yanhai; Yan, Zhen

    2013-11-07

    China has the highest incidence of hepatitis B virus (HBV) infection worldwide. HBV genotypes have variable impacts on disease pathogenesis and drug tolerance. We have developed a technically simple and accurate method for HBV genotyping that will be applicable to pre-treatment diagnosis and individualized treatment. Multiple sequence alignments of HBV genomes from GenBank were used to design primers and probes for genotyping of HBV A through H. The hybridization was carried out on nitrocellulose (NC) membranes with probes fixed in an array format, which was followed by hybrid amplification by an extension step with DNA polymerase to reinforce the double-stranded DNA hybrids on the NC membrane and subsequent visualization using an avidin-biotin system. Genotyping results were confirmed by DNA sequencing and bioinformatics analysis using the National Center for Biotechnology Information genotyping database, and compared with results from the line probe assay. The data show that multiple sequence alignment defined a 630 bp region in the HBV PreS and S regions that was suitable for genotyping. All genotyping significant single nucleotides in the region were defined. Two-hundred-and-ninety-one HBV-positive serum samples from Northwest Chinese patients were genotyped, and the genotyping rate from the new modified hybridization-extension method was 100% compared with direct sequencing. Compared with line probe assay, the newly developed method is superior, featuring reduced reaction time, lower risk of contamination, and increased accuracy for detecting single nucleotide mutation. In conclusion, a novel hybridization-extension method for HBV genotyping was established, which represents a new tool for accurate and rapid SNP detection that will benefit clinical testing.

  13. Apparent digestibility of Asian carp and common carp-derived fish meals in feeds for hybrid striped bass Morone saxatilis female x M. chrysops male and rainbow trout Oncorhynchus mykiss

    Science.gov (United States)

    Apparent digestibility coefficients (ADCs) of nutrients (crude protein, amino acids, crude lipid, fatty acids, and minerals) were determined for fish meals derived from menhaden, Asian carp (combination of silver and bighead carps), and common carp in feeds for hybrid striped bass and rainbow trout....

  14. Fluorescent in-situ hybridization--some of its applications in clinical cytogenetics.

    Science.gov (United States)

    Gole, L A; Bongso, A

    1997-11-01

    Fluorescent in-situ hybridization (FISH) is becoming more and more relevant as an important future tool in prenatal and pre-implantation genetic diagnosis and cancer cytogenetics. This review describes the FISH technique as applied to whole chromosome spreads and interphase cells and discusses its applications in clinical cytogenetics. Information is presented on the various types of probes and the subsequent hybridization and detection procedures. The potential use of this novel FISH technique in the diagnosis of numerical and structural chromosomal aberrations in routine karyotyping for prenatal diagnosis, tumour cytogenetics and pre-implantation genetic diagnosis is outlined.

  15. Metallomics investigations on potential binding partners of methylmercury in tuna fish muscle tissue using complementary mass spectrometric techniques.

    Science.gov (United States)

    Kutscher, Daniel J; Sanz-Medel, Alfredo; Bettmer, Jörg

    2012-08-01

    In this study, the binding behaviour of methylmercury (MeHg(+)) towards proteins is investigated. Free sulfhydryl groups in cysteine residues are known to be the most likely binding partners, due to the high affinity of mercury to sulphur. However, detailed knowledge about discrete binding sites in living organisms has been so far scarce. A metallomics approach using different methods like size-exclusion chromatography (SEC) and liquid chromatography (LC) coupled to inductively coupled plasma-mass spectrometry (ICP-MS) as well as complementary mass spectrometric techniques (electrospray ionisation-tandem mass spectrometry, ESI-MS/MS) are combined to sequence and identify possible target proteins or peptides after enzymatic digestion. Potential targets for MeHg(+) in tuna fish muscle tissue are investigated using the certified reference material CRM464 as a model tissue. Different extraction procedures appropriate for the extraction of proteins are evaluated for their efficiency using isotope dilution analysis for the determination of total Hg in the extracts. Due to the high chemical stability of the mercury-sulphur bond, the bioconjugate can be quantitatively extracted with a combination of tris(hydroxymethyl)aminomethane (TRIS) and sodium dodecyl sulphate (SDS). Using different separation techniques such as SEC and SDS-polyacrylamide gel electrophoresis (SDS-PAGE) it can be shown that major binding occurs to a high-molecular weight protein (M(w) > 200 kDa). A potential target protein, skeletal muscle myosin heavy chain, could be identified after tryptic digestion and capillary LC-ESI-MS/MS.

  16. Fully Automated Fluorescent in situ Hybridization (FISH) Staining and Digital Analysis of HER2 in Breast Cancer : A Validation Study

    NARCIS (Netherlands)

    van der Logt, Elise M. J.; Kuperus, Deborah A. J.; van Setten, Jan W.; van den Heuvel, Marius C.; Boers, James. E.; Schuuring, Ed; Kibbelaar, Robby E.

    2015-01-01

    HER2 assessment is routinely used to select patients with invasive breast cancer that might benefit from HER2-targeted therapy. The aim of this study was to validate a fully automated in situ hybridization (ISH) procedure that combines the automated Leica HER2 fluorescent ISH system for Bond with su

  17. Energy Spectrum of Ultra-High Energy Cosmic Rays Observed with the Telescope Array Using a Hybrid Technique

    CERN Document Server

    Abu-Zayyad, T; Allen, M; Anderson, R; Azuma, R; Barcikowski, E; Belz, J W; Bergman, D R; Blake, S A; Cady, R; Cheon, B G; Chiba, J; Chikawa, M; Cho, E J; Cho, W R; Fujii, H; Fujii, T; Fukuda, T; Fukushima, M; Hanlon, W; Hayashi, K; Hayashi, Y; Hayashida, N; Hibino, K; Hiyama, K; Honda, K; Iguchi, T; Ikeda, D; Ikuta, K; Inoue, N; Ishii, T; Ishimori, R; Ito, H; Ivanov, D; Iwamoto, S; Jui, C C H; Kadota, K; Kakimoto, F; Kalashev, O; Kanbe, T; Kasahara, K; Kawai, H; Kawakami, S; Kawana, S; Kido, E; Kim, H B; Kim, H K; Kim, J H; Kitamoto, K; Kitamura, S; Kitamura, Y; Kobayashi, K; Kobayashi, Y; Kondo, Y; Kuramoto, K; Kuzmin, V; Kwon, Y J; Lan, J; Lim, S I; Lundquist, J P; Machida, S; Martens, K; Matsuda, T; Matsuura, T; Matsuyama, T; Matthews, J N; Minamino, M; Miyata, K; Murano, Y; Myers, I; Nagasawa, K; Nagataki, S; Nakamura, T; Nam, S W; Nonaka, T; Ogio, S; Ohnishi, M; Ohoka, H; Oki, K; Oku, D; Okuda, T; Ono, M; Oshima, A; Ozawa, S; Park, I H; Pshirkov, M S; Rodriguez, D C; Roh, S Y; Rubtsov, G; Ryu, D; Sagawa, H; Sakurai, N; Sampson, A L; Scott, L M; Shah, P D; Shibata, F; Shibata, T; Shimodaira, H; Shin, B K; Shin, J I; Shirahama, T; Smith, J D; Sokolsky, P; Springer, R W; Stokes, B T; Stratton, S R; Stroman, T; Suzuki, S; Takahashi, Y; Takeda, M; Taketa, A; Takita, M; Tameda, Y; Tanaka, H; Tanaka, K; Tanaka, M; Thomas, S B; Thomson, G B; Tinyakov, P; Tkachev, I; Tokuno, H; Tomida, T; Troitsky, S; Tsunesada, Y; Tsutsumi, K; Tsuyuguchi, Y; Uchihori, Y; Udo, S; Ukai, H; Urban, F; Vasiloff, G; Wada, Y; Wong, T; Yamakawa, Y; Yamane, R; Yamaoka, H; Yamazaki, K; Yang, J; Yoneda, Y; Yoshida, S; Yoshii, H; Zhou, X; Zollinger, R; Zundel, Z

    2013-01-01

    We measure the spectrum of cosmic rays with energies greater than $10^{18.2}$ eV with the Fluorescence Detectors (FDs) and the Surface Detectors (SDs) of the Telescope Array Experiment using the data taken in our first 2.3-year observation from May 27 2008 to September 7 2010. A hybrid air shower reconstruction technique is employed to improve accuracies in determination of arrival directions and primary energies of cosmic rays using both FD and SD data. The energy spectrum presented here is in agreement with our previously published spectra and the HiRes results.

  18. ADAPTING HYBRID MACHINE TRANSLATION TECHNIQUES FOR CROSS-LANGUAGE TEXT RETRIEVAL SYSTEM

    Directory of Open Access Journals (Sweden)

    P. ISWARYA

    2017-03-01

    Full Text Available This research work aims in developing Tamil to English Cross - language text retrieval system using hybrid machine translation approach. The hybrid machine translation system is a combination of rule based and statistical based approaches. In an existing word by word translation system there are lot of issues and some of them are ambiguity, Out-of-Vocabulary words, word inflections, and improper sentence structure. To handle these issues, proposed architecture is designed in such a way that, it contains Improved Part-of-Speech tagger, machine learning based morphological analyser, collocation based word sense disambiguation procedure, semantic dictionary, and tense markers with gerund ending rules, and two pass transliteration algorithm. From the experimental results it is clear that the proposed Tamil Query based translation system achieves significantly better translation quality over existing system, and reaches 95.88% of monolingual performance.

  19. Application of hybrid microwave thermal extraction techniques for mulberry root bark

    OpenAIRE

    Wang Cheng-Chi; Yau Her-Terng

    2013-01-01

    The main focus of this paper is the extraction of compounds from the mulberry root bark using a hybrid microwave thermal process. The shearing mechanism and an integrated circulation system, which increases the rate of contact between the solvent and extractive, are studied. The results are analyzed by the Taguchi method and verified by high performance liquid chromatography. Furthermore, the optimal operating parameters of the extraction of mulberry root b...

  20. Development and Application of Nucleic Acid Hybridization Techniques to Arbovirus Surveillance and Diagnosis.

    Science.gov (United States)

    1986-11-04

    The pVV9 and pWV7 probes were more specific for dengue-2 RNA, but-o ::e cross reaction with other dengue serotypes was noted. ,PdUc1biLity Codes S...contained dengue specific inserts that strongly hybridized to dengue-2 RMA and to the RNA of other dengue serotypes to varying degrees (Figure 6). The pVVl

  1. FPGA Techniques Based New Hybrid Modulation Strategies for Voltage Source Inverters

    OpenAIRE

    Sudha, L. U.; J. Baskaran; Elankurisil, S. A.

    2015-01-01

    This paper corroborates three different hybrid modulation strategies suitable for single-phase voltage source inverter. The proposed method is formulated using fundamental switching and carrier based pulse width modulation methods. The main tale of this proposed method is to optimize a specific performance criterion, such as minimization of the total harmonic distortion (THD), lower order harmonics, switching losses, and heat losses. The proposed method is articulated using fundamental switch...

  2. Application of DNA hybridization techniques in the assessment of diarrheal disease among refugess in Thailand. [Shigella; Escherichia coli; Campylobacter; Cryptosporidium

    Energy Technology Data Exchange (ETDEWEB)

    Taylor, D.N.; Echeverria, P.; Pitarangsi, C.; Seriwatana, J.; Sethabutr, O.; Bodhidatta, L.; Brown, C.; Herrmann, J.E.; Blacklow, N.R.

    1988-01-01

    The epidemiology and etiology of acute diarrheal disease were determined in a Hmong refugee camp on the Thai-Laotian border from April 11 to May 14, 1985. DNA hybridization techniques were used to detect Shigella species, enteroinvasive Escherichia coli, and enterotoxigenic E. coli. A monoclonal enzyme-linked immunosorbent assay was used to detect rotavirus, and standard microbiology was used to detect other enteropathogens. The age-specific diarrheal disease rates were 47 episodes per month per 1000 children less than five years old and 113 episodes per month per 1000 children less than one year old. Rotavirus, enterotoxigenic E. coli, Campylobacter, and Cryptosporidium were the predominant pathogens in children less than two years old. The DNA probe hybridized with 94% of 31 specimens identified as enterotoxigenic E. coli by the standard assays and with none of the specimens in which the standard assays were negative. The probe for Shigella and enteroinvasive E. coli hybridized in eight of 10 stools that contained Shigella and four of 314 stools from which Shigella and enteroinvasive E. coli were not isolated. The use of DNA probes allows specimens to be collected in remote areas with a minimum amount of equipment and technical expertise so that they can be easily transported to a central laboratory for further processing.

  3. Screening of FOXP3-interacted proteins by yeast two-hybrid technique

    Institute of Scientific and Technical Information of China (English)

    Zhou Lina; Wu Jun; Luo Gaoxing; He Weifeng; Chen Xiwei; Bo Ganping; Yuan Shunzong; Zhang Xiaorong; Hu Xiaohong

    2008-01-01

    Objective: To screen the proteins interacting with the Treg specification factor forkhead box protein P3 (FOXP3) by yeast two-hybrid system. Methods: Human FOXP3 gene was amplified by nest RT-PCR from peripheral blood mononuclear cells (PBMC) and inserted into plasmid pGBKT7 to construct the bait vector, then the self-activation and toxicity of the bait vector in host yeast strain AH109 were observed. Thereafter, a human liver cDNA library was screened by the bait vector. The positive clones were selected out by nutrient-deficient culture and back-hybridizing. The sequences from the candidate positive clones were blasted and analyzed by bioinformatics methods. Results: The constructed bait vector encoding FOXP3 was found no self-activation and toxicity in yeast AH109. Three proteins which interacted with FOXP3, including tumor protein D52, splicing factor 3b subunit 1 and hypothetical protein, were identified. Conclusion: Three new candidate proteins interacting with FOXP3 are selected out by this yeast two-hybrid system and library, which may facilitate the further study of FOXP3 in Treg.

  4. Comprehensive meiotic segregation analysis of a 4-breakpoint t(1;3;6) complex chromosome rearrangement using single sperm array comparative genomic hybridization and FISH.

    Science.gov (United States)

    Hornak, Miroslav; Vozdova, Miluse; Musilova, Petra; Prinosilova, Petra; Oracova, Eva; Linkova, Vlasta; Vesela, Katerina; Rubes, Jiri

    2014-10-01

    Complex chromosomal rearrangements (CCR) represent rare structural chromosome abnormalities frequently associated with infertility. In this study, meiotic segregation in spermatozoa of an infertile normospermic carrier of a 4-breakpoint t(1;3;6) CCR was analysed. A newly developed array comparative genomic hybridization protocol was used, and all chromosomes in 50 single sperm cells were simultaneously examined. Three-colour FISH was used to analyse chromosome segregation in 1557 other single sperm cells. It was also used to measure an interchromosomal effect; sperm chromatin structure assay was used to measure chromatin integrity. A high-frequency of unbalanced spermatozoa (84%) was observed, mostly arising from the 3:3 symmetrical segregation mode. Array comparative genomic hybridization was used to detect additional aneuploidies in two out of 50 spermatozoa (4%) in chromosomes not involved in the complex chromosome rearrangement. Significantly increased rates of diploidy and XY disomy were found in the CCR carrier compared with the control group (P < 0.001). Defective condensation of sperm chromatin was also found in 22.7% of spermatozoa by sperm chromatin structure assay. The results indicate that the infertility in the man with CCR and normal spermatozoa was caused by a production of chromosomally unbalanced, XY disomic and diploid spermatozoa and spermatozoa with defective chromatin condensation.

  5. Towards Fluorescence In Vivo Hybridization (FIVH) Detection of H. pylori in Gastric Mucosa Using Advanced LNA Probes

    DEFF Research Database (Denmark)

    Fontenete, Sílvia; Leite, Marina; Guimarães, Nuno

    2015-01-01

    acid (LNA)/ 2' O-methyl RNA (2'OMe) probe using standard phosphoramidite chemistry and FISH hybridization was then successfully performed both on adhered and suspended bacteria at 37°C. In this work we simplified, shortened and adapted FISH to work at gastric pH values, meaning that the hybridization......In recent years, there have been several attempts to improve the diagnosis of infection caused by Helicobacter pylori. Fluorescence in situ hybridization (FISH) is a commonly used technique to detect H. pylori infection but it requires biopsies from the stomach. Thus, the development of an in vivo...

  6. Collision avoidance for a mobile robot based on radial basis function hybrid force control technique

    Institute of Scientific and Technical Information of China (English)

    Wen Shu-Huan

    2009-01-01

    Collision avoidance is always difficult in the planning path for a mobile robot. In this paper, the virtual force field between a mobile robot and an obstacle is formed and regulated to maintain a desired distance by hybrid force control algorithm. Since uncertainties from robot dynamics and obstacle degrade the performance of a collision avoidance task, intelligent control is used to compensate for the uncertainties. A radial basis function (RBF) neural network is used to regulate the force field of an accurate distance between a robot and an obstacle in this paper and then simulation studies are conducted to confirm that the proposed algorithm is effective.

  7. GENERAL: Collision avoidance for a mobile robot based on radial basis function hybrid force control technique

    Science.gov (United States)

    Wen, Shu-Huan

    2009-10-01

    Collision avoidance is always difficult in the planning path for a mobile robot. In this paper, the virtual force field between a mobile robot and an obstacle is formed and regulated to maintain a desired distance by hybrid force control algorithm. Since uncertainties from robot dynamics and obstacle degrade the performance of a collision avoidance task, intelligent control is used to compensate for the uncertainties. A radial basis function (RBF) neural network is used to regulate the force field of an accurate distance between a robot and an obstacle in this paper and then simulation studies are conducted to confirm that the proposed algorithm is effective.

  8. An alternative hybrid evolutionary technique focused on allocating machines and sequencing operations

    Directory of Open Access Journals (Sweden)

    Mariano Frutos

    2016-09-01

    Full Text Available We present here a hybrid algorithm for the Flexible Job-Shop Scheduling Problem (FJSSP. This problem involves the optimal use of resources in a flexible production environment in which each operation can be carried out by more than a single machine. Our algorithm allocates, in a first step, the machines to operations and in a second stage it sequences them by integrating a Multi-Objective Evolutionary Algorithm (MOEA and a path-dependent search algorithm (Multi-Objective Simulated Annealing, which is enacted at the genetic phase of the procedure. The joint interaction of those two components yields a very efficient procedure for solving the FJSSP. An important step in the development of the algorithm was the selection of the right MOEA. Candidates were tested on problems of low, medium and high complexity. Further analyses showed the relevance of the search algorithm in the hybrid structure. Finally, comparisons with other algorithms in the literature indicate that the performance of our alternative is good.

  9. STUDY OF MECHANICAL PROPERTIES OF BANANA-COIR HYBRID COMPOSITE USING EXPERIMENTAL AND FEM TECHNIQUES

    Directory of Open Access Journals (Sweden)

    T. Hariprasad

    2013-06-01

    Full Text Available The use of natural fibers as reinforcement in polymers has gained importance in recent years due to their eco-friendly nature. Thus, an investigation has been undertaken on banana-coir, which is a natural fiber abundantly available in India. Natural fibers are not only strong and lightweight, but also relatively very cheap. Composite plates were prepared with resin 392 g, coir 54 g, and banana 69 g. The purpose of this work is to establish the tensile, flexural, and impact properties of banana-coir reinforced composite materials with a thermo set for treated and untreated fibers. The resin used was epoxy (EP306. The tensile and impact tests showed that treated banana-coir epoxy hybrid composites have higher tensile strength and impact strength than untreated composites. However, untreated fiber composites have greater flexural strength than the treated fiber composites. The finite element analysis (FEA software ANSYS has been employed successfully to evaluate the properties. The stresses at the interface of the banana-coir and matrix, induced by the different loading conditions, were applied to predict the tensile, impact, and flexural properties by using the FEA models. The model output was compared with the experimental results and found to be close. This analysis is useful for realizing the advantages of hybrid fiber reinforced composites in structural applications and for identifying where the stresses are critical and damage the interface under varying loading conditions.

  10. Improved performance of hybrid error control techniques for real-time digital communications over noisy channels

    Science.gov (United States)

    Yang, Charlie Qing

    1993-06-01

    Delay-related performance characteristics are investigated for asynchronous time division multiplexing links. Two methods based on an imbedded Markov chain model are developed and applied to the system with a noisy feedback channel yielding analytical expressions for the buffer occupancy and the block delay. A recursive expression for packet loss probability for systems with a finite transmitter buffer is obtained. The concept of delay limited error control coding is introduced for real-time communications. Performance improvement by truncation of a type-2 hybrid automatic repeat-request (ARQ) protocol with one retransmission is examined showing that the truncated protocol has a bounded delay and bounded queue length under typical conditions. The error performance of the truncated protocol is further analyzed for various mobile fading channels. Matched rate hybrid error control coding for both adaptive and non-adaptive cases is also studied. A new adaptive error control protocol using Reed-Solomon codes is proposed using novel feedback transmissions to achieve faster estimation of channel states. Numerical optimization is carried out by introducing overall and modified throughput as efficiency criteria. Based on channel bit error rate measurement, optimum overall throughput is obtained with minimum implementation complexity.

  11. Hybrid technique coil embolisation for intrahepatic arterioportal fistula in a cat: case report

    Directory of Open Access Journals (Sweden)

    Akiko Uemura

    2016-04-01

    Full Text Available Case summary A 13-month-old, female, mixed breed, 4.0 kg cat was referred with a 6 month history of decreased appetite, loss of vigour and intermittent vomiting. Physical examination revealed no cyanosis or wasting, and no audible heart murmur was auscultated. Blood profile revealed mild anaemia and mildly elevated postprandial serum ammonia (109 µg/dl. Abdominal ultrasonography revealed dilation of an intrahepatic portal vein branch and an intrahepatic aneurysm, with splenomegaly and ascites. Hepatic arteriovenous fistula/hepatic artery–portal vein fistula with multiple acquired portosystemic shunts was strongly suspected. Medical control was achieved using antibiotics, liver-protecting agents, a low-protein diet and blood transfusions. However, because medical treatment proved ineffective, coil embolisation was performed on day 11, using a hybrid approach via the mesenteric vein. Subsequent follow-up showed good appetite, with no signs of diarrhoea or ascites. Abdominal ultrasonography revealed that the mosaic pattern around the site of coil placement in the portal vein branch had improved and pulsatility had disappeared. Relevance and novel information Intrahepatic arterioportal fistula involves a circulatory shunt between the hepatic artery and the hepatic or portal vein within the liver, and may be congenital or acquired. Both forms have been reported in humans, but most cases in cats have been congenital. Few reports have described treatment methods or prognosis in cats. We report here that coil embolisation using a hybrid approach is a procedure offering easy, effective treatment by blocking hepatofugal blood flow.

  12. DRYING OF EMPTY FRUIT BUNCHES AS WASTED BIOMASS BY HYBRID SOLAR–THERMAL DRYING TECHNIQUE

    Directory of Open Access Journals (Sweden)

    H. H. Al-Kayiem

    2013-12-01

    Full Text Available Solar drying of EFB is highly feasible and economic, but the solar drying process is interrupted during cloudy or rainy days and also at night. In the present paper, a combined solar, as the main heat input, and biomass burner, as an auxiliary source of thermal energy, has been investigated experimentally to dry EFB. An experimental model consisting of a solar dryer integrated with a thermal backup unit was designed and fabricated. A series of experimental measurements were carried out in four different drying modes, namely, open sun, mixed direct and indirect solar, thermal backup, and hybrid. The results from the four modes used to dry 2.5 kg of EFB were summarized and compared. The results indicated that the solar drying mode required around 52 to 80 hours to dry the EFB, while the open sun drying mode required 100 hours. Usage of the thermal backup as heat source reduced the drying time to 48–56 hours. With the hybrid mode, the drying time was considerably reduced to 24–32 hours. The results demonstrate that the combined solar and thermal backup effectively enhanced the drying performance. The application of a solar dryer with a biomass burner is practical for massive production of solid fuels from EFB.

  13. The use of genetic engineering techniques to improve the lipid composition in meat, milk and fish products: a review.

    Science.gov (United States)

    Świątkiewicz, S; Świątkiewicz, M; Arczewska-Włosek, A; Józefiak, D

    2015-04-01

    The health-promoting properties of dietary long-chain n-3 polyunsaturated fatty acids (n-3 LCPUFAs) for humans are well-known. Products of animal-origin enriched with n-3 LCPUFAs can be a good example of functional food, that is food that besides traditionally understood nutritional value may have a beneficial influence on the metabolism and health of consumers, thus reducing the risk of various lifestyle diseases such as atherosclerosis and coronary artery disease. The traditional method of enriching meat, milk or eggs with n-3 LCPUFA is the manipulation of the composition of animal diets. Huge progress in the development of genetic engineering techniques, for example transgenesis, has enabled the generation of many kinds of genetically modified animals. In recent years, one of the aims of animal transgenesis has been the modification of the lipid composition of meat and milk in order to improve the dietetic value of animal-origin products. This article reviews and discusses the data in the literature concerning studies where techniques of genetic engineering were used to create animal-origin products modified to contain health-promoting lipids. These studies are still at the laboratory stage, but their results have demonstrated that the transgenesis of pigs, cows, goats and fishes can be used in the future as efficient methods of production of healthy animal-origin food of high dietetic value. However, due to high costs and a low level of public acceptance, the introduction of this technology to commercial animal production and markets seems to be a distant prospect.

  14. Phenotypic consequences of a mosaic marker chromosome identified by fluorescence in situ hybridization (FISH) as being derived from chromosome 16

    Energy Technology Data Exchange (ETDEWEB)

    Ray, J.H.; Zhou, X.; Pletcher, B.A. [Cornell Univ. Medical College, Manhasset, NY (United States)] [and others

    1994-09-01

    De novo marker chromosomes are detected in 1 in 2500 amniotic fluid samples and are associated with a 10-15% risk for phenotypic abnormality. FISH can be utilized as a research tool to identify the origins of marker chromosomes. The phenotypic consequences of a marker chromosome derived from the short arm of chromosome 16 are described. A 26-year-old woman underwent amniocentesis at 28 weeks gestation because of a prenatally diagnosed tetralogy of Fallot. Follow-up ultrasounds also showed ventriculomegaly and cleft lip and palate. 32 of 45 cells had the karyotype 47,XY,+mar; the remaining cells were 46,XY. The de novo marker chromosome was C-band positive and non-satellited and failed to stain with distamycin A/DAPI. At birth the ultrasound findings were confirmed and dysmorphic features and cryptorchidism were noted. Although a newborn blood sample contained only normal cells, mosaicism was confirmed in 2 skin biopsies. FISH using whole-chromosome painting and alpha-satellite DNA probes showed that the marker chromosome had originated from chromosome 16. As proximal 16q is distamycin A/DAPI positive, the marker is apparently derived from proximal 16p. At 15 months of age, this child is hypotonic, globally delayed and is gavage-fed. His physical examination is significant for microbrachycephaly, a round face, sparse scalp hair, ocular hypertelorism, exotropia, a flat, wide nasal bridge and tip, mild micrognathia, and tapered fingers with lymphedema of hands and feet. Inguinal hernias have been repaired. His features are consistent with those described for patients trisomic for most or all of the short arm of chromosome 16. Marker chromosomes derived from the short arm of chromosome 16 appear to have phenotypic consequences. As the origin of more marker chromosomes are identified using FISH, their karyotype/phenotype correlations will become more apparent, which will permit more accurate genetic counseling.

  15. Suppression subtraction hybridization (SSH) and macroarray techniques reveal differential gene expression profiles in brain of sea bream infected with nodavirus.

    Science.gov (United States)

    Dios, S; Poisa-Beiro, L; Figueras, A; Novoa, B

    2007-03-01

    Despite of the impact that viruses have on aquatic organisms, relatively little is known on how fish fight against these infections. In this work, the brain gene expression pattern of sea bream (Sparus aurata) in response to nodavirus infection was investigated. We used the suppression subtractive hybridization (SSH) method to generate a subtracted cDNA library enriched with gene transcripts differentially expressed after 1 day post-infection. Some of the ESTs from the infected tissues fell in gene categories related to stress and immune responses. For the reverse library (ESTs expressed in controls compared with infected tissues) the most abundant transcripts were of ribosomal and mitochondrial nature. Several ESTs potentially induced by virus exposure were selected for in vivo expression studies. We observed a clear difference in expression between infected and control samples for two candidate genes, ubiquitin conjugating enzyme 7 interacting protein, which seems to play an important role in apoptosis and the interferon induced protein with helicase C domain 1 (mda-5) that contributes to apoptosis and regulates the type I IFN production, a key molecule of the antiviral innate response in most organisms.

  16. Active control of broadband sound transmission through an airplane trim panel using hybrid feedforward and feedback techniques

    Science.gov (United States)

    Ho, Yun-Ren

    This thesis presents a method of actively controlling the sound transmission through two designs of aircraft trim panels using a hybrid feedforward and feedback control technique. The active trim panels are designed for a high stiffness to mass ratio to allow only rigid body modal vibration in the frequency range of interest, thus simplifying the control technique, which is developed to minimize the vibration of a panel and therefore minimize the sound transmission. The hybrid controller consists of an adaptive feedforward (filtered-X LMS algorithm) controller in conjunction with a linear quadratic Gaussian (LQG) feedback controller. The LQG feedback controller is designed to alter the dynamics of the estimated plant model of the filtered-x LMS algorithm, improving control performance for both steady state and transient disturbances. Numerical simulations indicate that the hybrid controller is a more effective method of reducing the vibrations of the panels (and therefore the sound transmission) when compared to using only a feedforward or feedback controller. Experiments were carried out by using two trim panel designs, the first exhibiting only an out-of-plane piston mode, and the second exhibiting three rigid body modes and the first bending mode in control frequency range. For the first trim panel, the implementation of the active control experiment showed that a 5 to 20 dB reduction in both the vibration level and sound pressure level could be achieved over 50 to 500 Hz under a plane acoustic wave excitation. For the second trim panel, the hybrid controller achieved a 5 to 20 dB vibration reduction over the 50 to 400 Hz frequency band under structure-borne excitation. For air-borne excitation, the control scheme produced a 5 to 15 dB vibration reduction over the 70 to 400 Hz bandwidth with a reference microphone attached on the center of the fuselage skin and facing the sound source. In the near field (50 cm from the panel), the sound pressure levels measured

  17. Le diagnostic anténatal de la trisomie 21 par l'hybridation in situ en fluorescence (FISH): à propos des premiers tests réalisés au Maroc

    Science.gov (United States)

    Lamzouri, Afaf; Natiq, Abdelhafid; Tajir, Mariam; Sendid, Mohamed; Sefiani, Abdelaziz

    2012-01-01

    Introduction Le but de cette étude était de présenter les premiers résultats de diagnostic anténatal de la trisomie 21 par la technique d'hybridation in situ en fluorescence (FISH) au Maroc et discuter son intérêt dans le diagnostic rapide de cette aneuploïdie. Méthodes Ce travail a été réalisé chez 23 femmes avec des grossesses à haut risque de trisomie 21. La moyenne d’âge des gestantes étaient de 37,43 ans avec des extrêmes de 21 et 43 ans. Toutes étaient musulmanes mariées, mariage légitimé par la Charia, dont trois mariages consanguins, sauf une originaire de la République Démocratique du Congo qui était chrétienne et concubine. La majorité des femmes étaient fonctionnaires et avaient un niveau de scolarisation moyen à élevé. Toutes les patientes ont bénéficié d'une consultation de génétique médicale au cours de laquelle il leur a été donné des informations sur la technique, son intérêt et ses limites. Il s'agit de femmes enceintes qui avaient soit un âge maternel élevé ou des signes d'appel échographiques et/ ou biochimiques. Une des patientes était porteuse d'une translocation robertsonienne t(14;21) équilibrée. Une amniocentèse a été réalisée chez toutes les gestantes et aucun avortement n'a était induit par ce geste invasif. L’âge gestationnel moyen à la première consultation était de 14 semaines d'aménorrhée (SA) et à l'amniocentèse était de 16 SA et 5 jours. L'analyse FISH a été réalisée, après consentement des couples, sur des cellules non cultivées à partir des échantillons de liquides amniotiques, en utilisant des sondes spécifiques du chromosome 21. Résultats Parmi les 23 patientes qui ont bénéficiées d'un diagnostic anténatal de la trisomie 21 par la technique FISH, nous avons pu rassurer 21 d'entre elles, et nous avons détecté deux cas de trisomie 21 fœtal. Conclusion La technique FISH permet un diagnostic anténatal rapide, en moins de 48h, de la trisomie 21 sur

  18. Hybrid Neural-Network: Genetic Algorithm Technique for Aircraft Engine Performance Diagnostics Developed and Demonstrated

    Science.gov (United States)

    Kobayashi, Takahisa; Simon, Donald L.

    2002-01-01

    As part of the NASA Aviation Safety Program, a unique model-based diagnostics method that employs neural networks and genetic algorithms for aircraft engine performance diagnostics has been developed and demonstrated at the NASA Glenn Research Center against a nonlinear gas turbine engine model. Neural networks are applied to estimate the internal health condition of the engine, and genetic algorithms are used for sensor fault detection, isolation, and quantification. This hybrid architecture combines the excellent nonlinear estimation capabilities of neural networks with the capability to rank the likelihood of various faults given a specific sensor suite signature. The method requires a significantly smaller data training set than a neural network approach alone does, and it performs the combined engine health monitoring objectives of performance diagnostics and sensor fault detection and isolation in the presence of nominal and degraded engine health conditions.

  19. Enhancement of the mechanical properties of an aluminum metal matrix nanocomposite by the hybridization technique

    Directory of Open Access Journals (Sweden)

    Kalidindi Sita Rama Raju

    2016-07-01

    Full Text Available A uniform distribution of nanoparticles in the matrix plays a prominent role in improving the composite strength. In the present investigation, two types of launching vehicles, such as aluminum powder (primary and CNTs (secondary, are considered to uniformly carry and launch ultra-fine nanoparticles (13 nm into molten metal. The use of a secondary launching vehicle is identified to promote strengthening compared to a regular primary vehicle, as indicated by the good distribution observed from electron micrographs. CNTs are responsible for hybridizing the composite and also assist strengthening by anchoring to the matrix through the destroyed outer-walls and their axial orientation with the matrix. These results help us in attaining a strength of 197 MPa and a hardness of 93 BHN, with a minimal loss in ductility for the H-3 sample.

  20. FPGA techniques based new hybrid modulation strategies for voltage source inverters.

    Science.gov (United States)

    Sudha, L U; Baskaran, J; Elankurisil, S A

    2015-01-01

    This paper corroborates three different hybrid modulation strategies suitable for single-phase voltage source inverter. The proposed method is formulated using fundamental switching and carrier based pulse width modulation methods. The main tale of this proposed method is to optimize a specific performance criterion, such as minimization of the total harmonic distortion (THD), lower order harmonics, switching losses, and heat losses. The proposed method is articulated using fundamental switching and carrier based pulse width modulation methods. Thus, the harmonic pollution in the power system will be reduced and the power quality will be augmented with better harmonic profile for a target fundamental output voltage. The proposed modulation strategies are simulated in MATLAB r2010a and implemented in a Xilinx spartan 3E-500 FG 320 FPGA processor. The feasibility of these modulation strategies is authenticated through simulation and experimental results.

  1. Fusion techniques for hybrid ground-penetrating radar: electromagnetic induction landmine detection systems

    Science.gov (United States)

    Laffin, Matt; Mohamed, Magdi A.; Etebari, Ali; Hibbard, Mark

    2010-04-01

    Hybrid ground penetrating radar (GPR) and electromagnetic induction (EMI) sensors have advanced landmine detection far beyond the capabilities of a single sensing modality. Both probability of detection (PD) and false alarm rate (FAR) are impacted by the algorithms utilized by each sensing mode and the manner in which the information is fused. Algorithm development and fusion will be discussed, with an aim at achieving a threshold probability of detection (PD) of 0.98 with a low false alarm rate (FAR) of less than 1 false alarm per 2 square meters. Stochastic evaluation of prescreeners and classifiers is presented with subdivisions determined based on mine type, metal content, and depth. Training and testing of an optimal prescreener on lanes that contain mostly low metal anti-personnel mines is presented. Several fusion operators for pre-screeners and classifiers, including confidence map multiplication, will be investigated and discussed for integration into the algorithm architecture.

  2. Technique of performing construction works by machines with hybrid: manual and remote control

    Directory of Open Access Journals (Sweden)

    Sevryugina Nadezhda

    2017-01-01

    Full Text Available The article discusses issues dealing with efficiency of construction work mechanization. It offers a mathematical model for assessment of mutual influence between the members of the ‘construction site-machine-operator’ system triad, that can give a quantitative assessment of how the efficiency of a technological task varies with more comprehensive use of operational capacities of the machine, while lower effect that limiting parameters of production environment and technical condition of the machine have on the operator. The article contains a constructive remote control solution for upgrade of the base machine. It describes the conditions for using the machines with hybrid: manual and remote control at construction sites. There is also an imitation model of operator’s scanning pattern and data experimental research that prove the efficiency of remotely controlled technological operations. The article proves that lower psychological load on the operator and better comfort contribute to positive economic effect and higher quality of the construction process.

  3. Wind Power Forecasting techniques in complex terrain: ANN vs. ANN-CFD hybrid approach

    Science.gov (United States)

    Castellani, Francesco; Astolfi, Davide; Mana, Matteo; Burlando, Massimiliano; Meißner, Cathérine; Piccioni, Emanuele

    2016-09-01

    Due to technology developments, renewable energies are becoming competitive against fossil sources and the number of wind farms is growing, which have to be integrated into power grids. Therefore, accurate power forecast is needed and often operators are charged with penalties in case of imbalance. Yet, wind is a stochastic and very local phenomenon, and therefore hard to predict. It has a high variability in space and time and wind power forecast is challenging. Statistical methods, as Artificial Neural Networks (ANN), are often employed for power forecasting, but they have some shortcomings: they require data sets over several years and are not able to capture tails of wind power distributions. In this work a pure ANN power forecast is compared against a hybrid method, based on the combination of ANN and a physical method using computational fluid dynamics (CFD). The validation case is a wind farm sited in southern Italy in a very complex terrain, with a wide spread turbine layout.

  4. A hybrid fringe analysis technique for the elimination of random noise in interferometric wrapped phase maps

    Science.gov (United States)

    Bhat, Gopalakrishna K.

    1994-10-01

    A fringe analysis technique, which makes use of the spatial filtering property of the Fourier transform method, for the elimination of random impulsive noise in the wrapped phase maps obtained using the phase stepping technique, is presented. Phase noise is converted into intensity noise by transforming the wrapped phase map into a continuous fringe pattern inside the digital image processor. Fourier transform method is employed to filter out the intensity noise and recover the clean wrapped phase map. Computer generated carrier fringes are used to preserve the sign information. This technique makes the two dimensional phase unwrapping process less involved, because it eliminates the local phase fluctuations, which act as pseudo 2π discontinuities. The technique is applied for the elimination of noise in a phase map obtained using electro-optic holography.

  5. Prediction of daily rainfall by a hybrid wavelet-season-neuro technique

    Science.gov (United States)

    Altunkaynak, Abdusselam; Nigussie, Tewodros Assefa

    2015-10-01

    Accurate daily rainfall prediction is required for accurate streamflow prediction, flooding risk analysis, constructing a reliable flood control and early warning system. However, because of its nonlinearity, prediction of daily rainfall with high accuracy and long prediction lead time is difficult. There are many daily rainfall prediction methods in the literature, but they are known to yield inaccurate predictions with short lead time, require many physical parameters and involve complicated mathematical equations with huge computational burden. Recently, artificial neural network has been used for predicting rainfall with the objective of addressing the above mentioned problems. But still, the accuracy has not been satisfactory and predictions are with short lead time. In this study, two methods called combined season-multilayer perceptron (SAS-MP) and hybrid wavelet-season-multilayer perceptron (W-SAS-MP) were developed to enhance prediction accuracy and extend prediction lead time of daily rainfall up to 5 days by using data from two stations in Turkey. These two models were compared with the stand-alone multilayer perceptron and another most commonly used method called combined wavelet-multilayer perceptron (W-MP). The performances of the models were evaluated by using coefficient of determination, coefficient of efficiency and root mean squared error. The SAS-MP model was found to be better than W-MP in most cases, except lead time day 1, where W-MP performed better. Throughout all the lead times, however, the hybrid W-SAS-MP model performed best with CE values of 0.911 and 0.909, respectively, for prediction lead time of 1 day and 0.588 and 0.570, respectively, for prediction lead time of 5 days at Stations 17836 and 17837, respectively, at the model testing (validation) phase. Therefore, W-SAS-MP can be an appropriate tool for enhancing daily rainfall prediction accuracy and extend prediction lead time.

  6. Distributed-parameter problem solved on a hybrid computer by a modified function storage technique

    DEFF Research Database (Denmark)

    Poulsen, N.J.

    1969-01-01

    Describes a method which is based on a function storage technique improved by a number of modifications; it fulfils the two main demands: the solution is based directly on the physical equations, and is accurate within 1%......Describes a method which is based on a function storage technique improved by a number of modifications; it fulfils the two main demands: the solution is based directly on the physical equations, and is accurate within 1%...

  7. A Low Cost Vision Based Hybrid Fiducial Mark Tracking Technique for Mobile Industrial Robots

    OpenAIRE

    Mohammed Y Aalsalem; Wazir Zada Khan; Quratul Ain Arshad

    2012-01-01

    The field of robotic vision is developing rapidly. Robots can react intelligently and provide assistance to user activities through sentient computing. Since industrial applications pose complex requirements that cannot be handled by humans, an efficient low cost and robust technique is required for the tracking of mobile industrial robots. The existing sensor based techniques for mobile robot tracking are expensive and complex to deploy, configure and maintain. Also some of them demand dedic...

  8. 荧光原位杂交技术在肾盂肿瘤诊断中的应用%Detection of renal pelvic tumor using fluorescence in situ hybridization technique

    Institute of Scientific and Technical Information of China (English)

    陈文轩; 林毅; 郭洪波; 李黎明

    2010-01-01

    目的 评价荧光原位杂交(FISH)技术在肾盂肿瘤诊断中的应用价值.方法 20例健康人通过FISH技术检测3、7、17号染色体及9号染色体p16位点的变异情况,建立阚值.15例影像学提示肾脏占位的血尿患者留取晨尿,分别进行尿脱落细胞学检查和FISH技术检测.以至少两种探针检测结果超过阈值或一种探针检测结果存在至少两种异常为诊断阳性.结果 15例血尿患者经手术及病理证实肾盂尿路上皮癌8例,肾透明细胞癌5例,肾嫌色细胞癌1例,黄色肉芽肿性肾盂肾炎1例.8例肾盂尿路上皮癌FISH技术检测均为阳性,3、7、17号染色体畸变阳性率均为100%(8/8),9号染色体p16位点畸变阳性率为75%(6/8).尿脱落细胞学检查仅1例阳性.7例非肾盂尿路上皮癌6例FISH技术检测阴性,而尿脱落细胞学检查则均为阴性.结论 FISH技术是一种无创快速诊断方法,在肾盂肿瘤诊断中优于尿脱落细胞学检查.%Objective To investigate the feasibihty and effectiveness of the fluorescence in situ hybridization(FISH)technique in the diagnosis of renal pelvic tumor.Methods FISH technique were used to detect the abnormalities of chromosome 3,7,17 and 9 p16 from 20 people without malignancy.The morning's first urine were availahled from 15 patients with hematuria and suspected of renal mass by imaging,then were studied by FISH technique and urine cytology respectively.The sample was considered positive if two or more probes results were higher than the criteria,or one probe had two or more abnormal results.Results Eight of 15 patients with hematuria were renal pelvic urothehal carcinoma,5 were renal clear cell carcinoma,1 was renal chromophobe cell carcinoma,1 was xanthogranulomatous pyelonephritis,confirmed by pathology.All of the 8 renal pelvic carcinoma were considered positive by FISH technique,while only 1 was considered positive by urine cytology.The variation rate of chromosome 3,7,17 were 100%(8/8)and

  9. A Review of Techniques for Fish Oil Application in Food Products%鱼油在食品领域中的应用技术综述

    Institute of Scientific and Technical Information of China (English)

    夏树华; 姜元荣; 张余权; 牛付欢

    2012-01-01

    This paper provides a review of tehcniques for the application of fish oil in foods products. Fish oil has been widely used in food products due to its special health benefit. It is necessary to add fish oil in food products because of deficient intake of docesahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) in China. Nowadays, there are many foods rich in fish oil avabiale in the market. The oxidative stability of fish oil has been a concern for a long time during this application. The addition styles such as direct addition, emulsion or microcapsulation, and new processing techniques can effectively improve the oxidative stability of fish oil. Therefore, the application of fish oil in daily food will be developed widely in future both technologically and commercially.%对鱼油在食品领域中的应用技术进行综述。由于具有独特的营养功能,鱼油的应用十分广泛。中国居民目前对二十碳五烯酸(EPA)和二十二碳六烯酸(DHA)摄入不足,因而鱼油在食品中的应用十分必要。目前市场上已有多种鱼油应用于各类食品中。在应用过程中,鱼油的氧化稳定性问题一直是研究热点,选择不同的添加形式(纯鱼油、乳状液和微胶囊)并采用某些新工艺可有效提高氧化稳定性。鱼油应用于食品在技术和商业方面的发展前景均十分广阔。

  10. [Evaluation of peptide nucleic acid fluorescent in situ hybridization (PNA FISH) method in the identifi cation of Candida species isolated from blood cultures].

    Science.gov (United States)

    Aydemir, Gonca; Koç, Ayşe Nedret; Atalay, Mustafa Altay

    2016-04-01

    In recent years, increased number of patients who are hospitalized in intensive care units, received immunosuppressive therapy and treated with broad-spectrum antibiotics that can lead an increase in the incidence of systemic candidiasis. In these patients, the most common clinical manifestation is candidemia. Since the identification of Candida species isolated from blood cultures is time consuming by conventional (morphological and biochemical) methods, rapid, reliable and accurate methods are needed. For this purpose novel systems have been developed to identify the agent directly. The aim of this study was to evaluate the peptide nucleic acid fluorescent in situ hybridization (PNA FISH) method for the identification of Candida species by comparing with the conventional methods. A total of 50 patients who were admitted to Erciyes University Medical Faculty Hospital clinics and followed with prediagnosis of systemic fungal infections whose blood cultures were positive for the yeasts between July 2011 and July 2012 were included in the study. The conventional identification of Candida isolates was performed by considering macroscopic and microscopic morphology, germ tube test, cycloheximide sensitivity, urease activity and carbohydrate assimilation patterns with API 20C AUX (bioMerieux, France) test. PNA FISH method was conducted by the use of a commercial kit namely Yeast Traffic Light(®) PNA FISH (AdvanDx, USA). According to morphological and biochemical characteristics (conventional methods), 19 (38%) out of 50 Candida isolates were identified as C.albicans, 12 (24%) as C.glabrata, five (10%) as C.parapsilosis, five (10%) as C.kefyr, four (8%) as C.krusei, two (4%) as C.guilliermondii, two (4%) as C.tropicalis and one (2%) as C.lusitaniae. On the other hand, 24 (48%) of the isolates were identified as C.albicans/C.parapsilosis (with green fluorescence), 16 (32%) as C.glabrata/C.krusei (with red fluorescence) and one (%2) as C.tropicalis (with yellow

  11. Applying Subtractive Hybridization Technique to Enrich and Amplify Tumor-Specific Transcripts of Esophageal Squamous Cell Carcinoma.

    Science.gov (United States)

    Mahmoudian, Reihaneh Alsadat; Abbaszadegan, Mohammad Reza; Gholamin, Mehran

    2017-04-01

    Subtractive hybridization (SH) as an efficient and powerful approach can be applied to isolate differentially expressed transcripts as well as detect of involved mRNAs in various cellular processes, particularly diseases and malignancies. This procedure leads to the enrichment of specific low copy transcripts of tumor cells. Having developed a new approach for SH to isolate tumor specific transcripts, we facilitated discovery of uniquely expressed genes in esophageal squamous cell carcinoma (ESCC). Total RNA was extracted from the fresh tumoral and their adjacent normal tissues, and purified using the Switch Mechanism At the 5' end of Reverse Transcript (SMART) method. Following cDNA synthesis of normal mRNAs using magnetic beads, it was hybridized with tumor mRNAs. To enhance efficiency of subtraction, hybridization was repeated three rounds. Finally, amplification of subtracted tumor-specific transcripts was carried out using in vitro transcription. The subtracted tumoral mRNAs was analyzed quantitatively using real-time PCR for both tumor-specific and housekeeping genes. The subtracted mRNA was confirmed as tumor-specific mRNA pool using RT-PCR and quantitative real-time PCR assessment. The elevated level of tumor-specific transcripts such as MAGE-A4 and CD44 as well as declined copy number of housekeeping genes such as GAPDH, β actin and β2-microglobulin, were confirmed in subtracted tumoral mRNA. The presence of tumor genes was confirmed after the SH procedure. The designed SH method in combination with SMART technique can isolate and amplify high quality tumor-specific transcripts even from small amount of tumor tissues. Removal of common transcripts from the extracted tumoral mRNAs using SH, leads to the enrichment of tumor-specific transcripts. The isolated transcripts are of interest because of their probable roles in ESCC progression and development. In addition, these tumor-specific mRNAs can be applied for future vaccine cancer studies.

  12. Electromagnetic self-consistent field initialization and fluid advance techniques for hybrid-kinetic PWFA code Architect

    Science.gov (United States)

    Massimo, F.; Marocchino, A.; Rossi, A. R.

    2016-09-01

    The realization of Plasma Wakefield Acceleration experiments with high quality of the accelerated bunches requires an increasing number of numerical simulations to perform first-order assessments for the experimental design and online-analysis of the experimental results. Particle in Cell codes are the state-of-the-art tools to study the beam-plasma interaction mechanism, but due to their requirements in terms of number of cores and computational time makes them unsuitable for quick parametric scans. Considerable interest has been shown thus in methods which reduce the computational time needed for the simulation of plasma acceleration. Such methods include the use of hybrid kinetic-fluid models, which treat the relativistic bunches as in a PIC code and the background plasma electrons as a fluid. A technique to properly initialize the bunch electromagnetic fields in the time explicit hybrid kinetic-fluid code Architect is presented, as well the implementation of the Flux Corrected Transport scheme for the fluid equations integrated in the code.

  13. Possibilities of LA-ICP-MS technique for the spatial elemental analysis of the recent fish scales: Line scan vs. depth profiling

    Energy Technology Data Exchange (ETDEWEB)

    Hola, Marketa [Department of Chemistry, Masaryk University of Brno, Kamenice 5, 625 00 Brno (Czech Republic); Kalvoda, Jiri, E-mail: jkalvoda@centrum.cz [Department of Geological Sciences, Masaryk University of Brno, Kotlarska 2, 611 37 Brno (Czech Republic); Novakova, Hana [Department of Chemistry, Masaryk University of Brno, Kamenice 5, 625 00 Brno (Czech Republic); Skoda, Radek [Department of Geological Sciences, Masaryk University of Brno, Kotlarska 2, 611 37 Brno (Czech Republic); Kanicky, Viktor [Department of Chemistry, Masaryk University of Brno, Kamenice 5, 625 00 Brno (Czech Republic)

    2011-01-01

    LA-ICP-MS and solution based ICP-MS in combination with electron microprobe are presented as a method for the determination of the elemental spatial distribution in fish scales which represent an example of a heterogeneous layered bone structure. Two different LA-ICP-MS techniques were tested on recent common carp (Cyprinus carpio) scales: (a)A line scan through the whole fish scale perpendicular to the growth rings. The ablation crater of 55 {mu}m width and 50 {mu}m depth allowed analysis of the elemental distribution in the external layer. Suitable ablation conditions providing a deeper ablation crater gave average values from the external HAP layer and the collagen basal plate. (b)Depth profiling using spot analysis was tested in fish scales for the first time. Spot analysis allows information to be obtained about the depth profile of the elements at the selected position on the sample. The combination of all mentioned laser ablation techniques provides complete information about the elemental distribution in the fish scale samples. The results were compared with the solution based ICP-MS and EMP analyses. The fact that the results of depth profiling are in a good agreement both with EMP and PIXE results and, with the assumed ways of incorporation of the studied elements in the HAP structure, suggests a very good potential for this method.

  14. Infection of Bacterial Endosymbionts in Insects: A Comparative Study of Two Techniques viz PCR and FISH for Detection and Localization of Symbionts in Whitefly, Bemisia tabaci.

    Directory of Open Access Journals (Sweden)

    Harpreet Singh Raina

    Full Text Available Bacterial endosymbionts have been associated with arthropods and large number of the insect species show interaction with such bacteria. Different approaches have been used to understand such symbiont- host interactions. The whitefly, Bemisia tabaci, a highly invasive agricultural pest, harbors as many as seven different bacterial endosymbionts. These bacterial endosymbionts are known to provide various nutritional, physiological, environmental and evolutionary benefits to its insect host. In this study, we have tried to compare two techniques, Polymerase chain reaction (PCR and Flourescence in situ Hybridisation (FISH commonly used for identification and localization of bacterial endosymbionts in B. tabaci as it harbors one of the highest numbers of endosymbionts which have helped it in becoming a successful global invasive agricultural pest. The amplified PCR products were observed as bands on agarose gel by electrophoresis while the FISH samples were mounted on slides and observed under confocal microscope. Analysis of results obtained by these two techniques revealed the advantages of FISH over PCR. On a short note, performing FISH, using LNA probes proved to be more sensitive and informative for identification as well as localization of bacterial endosymbionts in B. tabaci than relying on PCR. This study would help in designing more efficient experiments based on much reliable detection procedure and studying the role of endosymbionts in insects.

  15. Infection of Bacterial Endosymbionts in Insects: A Comparative Study of Two Techniques viz PCR and FISH for Detection and Localization of Symbionts in Whitefly, Bemisia tabaci

    Science.gov (United States)

    Raina, Harpreet Singh; Singh, Ambika; Popli, Sonam; Pandey, Neeti; Rajagopal, Raman

    2015-01-01

    Bacterial endosymbionts have been associated with arthropods and large number of the insect species show interaction with such bacteria. Different approaches have been used to understand such symbiont- host interactions. The whitefly, Bemisia tabaci, a highly invasive agricultural pest, harbors as many as seven different bacterial endosymbionts. These bacterial endosymbionts are known to provide various nutritional, physiological, environmental and evolutionary benefits to its insect host. In this study, we have tried to compare two techniques, Polymerase chain reaction (PCR) and Flourescence in situ Hybridisation (FISH) commonly used for identification and localization of bacterial endosymbionts in B. tabaci as it harbors one of the highest numbers of endosymbionts which have helped it in becoming a successful global invasive agricultural pest. The amplified PCR products were observed as bands on agarose gel by electrophoresis while the FISH samples were mounted on slides and observed under confocal microscope. Analysis of results obtained by these two techniques revealed the advantages of FISH over PCR. On a short note, performing FISH, using LNA probes proved to be more sensitive and informative for identification as well as localization of bacterial endosymbionts in B. tabaci than relying on PCR. This study would help in designing more efficient experiments based on much reliable detection procedure and studying the role of endosymbionts in insects. PMID:26287997

  16. Model Predictive Control techniques with application to photovoltaic, DC Microgrid, and a multi-sourced hybrid energy system

    Science.gov (United States)

    Shadmand, Mohammad Bagher

    Renewable energy sources continue to gain popularity. However, two major limitations exist that prevent widespread adoption: availability and variability of the electricity generated and the cost of the equipment. The focus of this dissertation is Model Predictive Control (MPC) for optimal sized photovoltaic (PV), DC Microgrid, and multi-sourced hybrid energy systems. The main considered applications are: maximum power point tracking (MPPT) by MPC, droop predictive control of DC microgrid, MPC of grid-interaction inverter, MPC of a capacitor-less VAR compensator based on matrix converter (MC). This dissertation firstly investigates a multi-objective optimization technique for a hybrid distribution system. The variability of a high-penetration PV scenario is also studied when incorporated into the microgrid concept. Emerging (PV) technologies have enabled the creation of contoured and conformal PV surfaces; the effect of using non-planar PV modules on variability is also analyzed. The proposed predictive control to achieve maximum power point for isolated and grid-tied PV systems speeds up the control loop since it predicts error before the switching signal is applied to the converter. The low conversion efficiency of PV cells means we want to ensure always operating at maximum possible power point to make the system economical. Thus the proposed MPPT technique can capture more energy compared to the conventional MPPT techniques from same amount of installed solar panel. Because of the MPPT requirement, the output voltage of the converter may vary. Therefore a droop control is needed to feed multiple arrays of photovoltaic systems to a DC bus in microgrid community. Development of a droop control technique by means of predictive control is another application of this dissertation. Reactive power, denoted as Volt Ampere Reactive (VAR), has several undesirable consequences on AC power system network such as reduction in power transfer capability and increase in

  17. All-optical delay technique for supporting multiple antennas in a hybrid optical - wireless transmission system

    DEFF Research Database (Denmark)

    Prince, Kamau; Chiuchiarelli, A; Presi, M

    2008-01-01

    We introduce a novel continuously-variable optical delay technique to support beam-forming wireless communications systems using antenna arrays. We demonstrate delay with 64-QAM modulated signals at a rate of 15 Msymbol/sec with 2.5 GHz carrier frequency.......We introduce a novel continuously-variable optical delay technique to support beam-forming wireless communications systems using antenna arrays. We demonstrate delay with 64-QAM modulated signals at a rate of 15 Msymbol/sec with 2.5 GHz carrier frequency....

  18. A Hybrid VLM Preceded SLM Technique Using Clipping and Filtering Method for PAPR Reduction in MIMO-OFDM Systems

    Directory of Open Access Journals (Sweden)

    Meenakshi

    2016-07-01

    Full Text Available MIMO-OFDM is an attractive interface for the next generation WLANs, WMAN, 4G and 5G mobile cellular systems. However the performance of the MIMO-OFDM systems is affected by Peak to Average Power Ratio (PAPR. PAPR is the main disadvantage associated with the MIMO-OFDM systems. So far, many techniques have been proposed to reduce the value of PAPR but high PAPR for MIMO-OFDM systems is still a demanding area and a different issue.In this paper, a hybrid VLM precoded SLM scheme using Clipping & Filtering has been proposed to reduce PAPR in MIMO-OFDM systems. And it has been observed that the proposed scheme has achieved a significant gain in PAPR reduction without increasing the system complexity and affecting the error performance of the system

  19. Therapeutic efficacy of a hybrid mandibular advancement device in the management of obstructive sleep apnea assessed with acoustic reflection technique

    Directory of Open Access Journals (Sweden)

    S S Agarwal

    2015-01-01

    Full Text Available Obstructive sleep apnea (OSA is one of the most common forms of sleep-disordered breathing. Various treatment modalities include behavior modification therapy, nasal continuous positive airway pressure (CPAP, oral appliance therapy, and various surgical modalities. Oral appliances are noninvasive and recommended treatment modality for snoring, mild to moderate OSA cases and severe OSA cases when patient is not compliant to CPAP therapy and unwilling for surgery. Acoustic reflection technique (ART is a relatively new modality for three-dimensional assessment of airway caliber in various clinical situations. The accuracy and reproducibility of acoustic rhinometry and acoustic pharyngometry assessment are comparable to computerized tomography and magnetic resonance imaging. This case report highlights the therapeutic efficacy of an innovative customized acrylic hybrid mandibular advancement device in the management of polysomnography diagnosed OSA cases, and the treatment results were assessed by ART.

  20. Hybrid vapor phase-solution phase growth techniques for improved CZT(S,Se) photovoltaic device performance

    Energy Technology Data Exchange (ETDEWEB)

    Chang, Liang-Yi; Gershon, Talia S.; Haight, Richard A.; Lee, Yun Seog

    2016-12-27

    A hybrid vapor phase-solution phase CZT(S,Se) growth technique is provided. In one aspect, a method of forming a kesterite absorber material on a substrate includes the steps of: depositing a layer of a first kesterite material on the substrate using a vapor phase deposition process, wherein the first kesterite material includes Cu, Zn, Sn, and at least one of S and Se; annealing the first kesterite material to crystallize the first kesterite material; and depositing a layer of a second kesterite material on a side of the first kesterite material opposite the substrate using a solution phase deposition process, wherein the second kesterite material includes Cu, Zn, Sn, and at least one of S and Se, wherein the first kesterite material and the second kesterite material form a multi-layer stack of the absorber material on the substrate. A photovoltaic device and method of formation thereof are also provided.

  1. Hybrid vapor phase-solution phase growth techniques for improved CZT(S,Se) photovoltaic device performance

    Science.gov (United States)

    Chang, Liang-Yi; Gershon, Talia S.; Haight, Richard A.; Lee, Yun Seog

    2016-12-27

    A hybrid vapor phase-solution phase CZT(S,Se) growth technique is provided. In one aspect, a method of forming a kesterite absorber material on a substrate includes the steps of: depositing a layer of a first kesterite material on the substrate using a vapor phase deposition process, wherein the first kesterite material includes Cu, Zn, Sn, and at least one of S and Se; annealing the first kesterite material to crystallize the first kesterite material; and depositing a layer of a second kesterite material on a side of the first kesterite material opposite the substrate using a solution phase deposition process, wherein the second kesterite material includes Cu, Zn, Sn, and at least one of S and Se, wherein the first kesterite material and the second kesterite material form a multi-layer stack of the absorber material on the substrate. A photovoltaic device and method of formation thereof are also provided.

  2. Treatment of breast cancer with simultaneous integrated boost in hybrid plan technique. Influence of flattening filter-free beams

    Energy Technology Data Exchange (ETDEWEB)

    Bahrainy, Marzieh; Kretschmer, Matthias; Joest, Vincent; Kasch, Astrid; Wuerschmidt, Florian; Dahle, Joerg; Lorenzen, Joern [Radiologische Allianz, Hamburg (Germany)

    2016-05-15

    The present study compares in silico treatment plans using hybrid plan technique during hypofractionated radiation of mammary carcinoma with simultaneous integrated boost (SIB). The influence of 6 MV photon radiation in flattening filter free (FFF) mode against the clinical standard flattening filter (FF) mode is to be examined. RT planning took place with FF and FFF radiation plans for 10 left-sided breast cancer patients. Hybrid plans were realised with two tangential IMRT fields and one VMAT field. The dose prescription was in line with the guidelines in the ARO-2010-01 study. The dosimetric verification took place with a manufacturer-independent measurement system. Required dose prescriptions for the planning target volumes (PTV) were achieved for both groups. The average dose values of the ipsi- and contralateral lung and the heart did not differ significantly. The overall average incidental dose to the left anterior descending artery (LAD) of 8.24 ± 3.9 Gy in the FFF group and 9.05 ± 3.7 Gy in the FF group (p < 0.05) were found. The dosimetric verifications corresponded to the clinical requirements. FFF-based RT plans reduced the average treatment time by 17 s/fraction. In comparison to the FF-based hybrid plan technique the FFF mode allows further reduction of the average LAD dose for comparable target volume coverage without adverse low-dose exposure of contralateral structures. The combination of hybrid plan technique and 6 MV photon radiation in the FFF mode is suitable for use with hypofractionated dose schemes. The increased dose rate allows a substantial reduction of treatment time and thus beneficial application of the deep inspiration breath hold technique. (orig.) [German] Vergleich der ''In-silico''-Bestrahlungsplaene der klinisch etablierten Hybridplan-Technik bei hypofraktionierter Bestrahlung des Mammakarzinoms mit simultan integriertem Boost (SIB). Untersucht wird der Einfluss von 6MV-Photonenstrahlung im Flattening

  3. Comparison and performance analysis of closed loop controlled nonlinear system connected PWM inverter based on hybrid technique

    Directory of Open Access Journals (Sweden)

    V.M. Deshmukh

    2015-05-01

    Full Text Available This paper proposed closed loop control of nonlinear system connected inverter based on the optimal neural controller (ONC. The novelty of the proposed method rests on the hybrid technique which is the combined performance of both, particle swarm optimization (PSO technique and Radial basis function neural network (RBFNN. It effectively optimizes the feasible solutions by updating the generations, by taking lesser time with greater reliability. In the proposed method, the PSO generates the dataset according to different loading conditions. The RBFNN is trained by using the target control signals along with the corresponding input load voltage error and change in error. Depending on the load variations, the RBFNN predicts the exact control signals of the inverter during the testing time. Since experimentation and comparison of such inverter models on hardware being relatively expensive, the proposed method is implemented in the MATLAB/Simulink platform and the performance has been validated through the comparison analysis with the conventional techniques. The comparison results have proved the superiority of the proposed method.

  4. 3D printing of high-resolution PLA-based structures by hybrid electrohydrodynamic and fused deposition modeling techniques

    Science.gov (United States)

    Zhang, Bin; Seong, Baekhoon; Nguyen, VuDat; Byun, Doyoung

    2016-02-01

    Recently, the three-dimensional (3D) printing technique has received much attention for shape forming and manufacturing. The fused deposition modeling (FDM) printer is one of the various 3D printers available and has become widely used due to its simplicity, low-cost, and easy operation. However, the FDM technique has a limitation whereby its patterning resolution is too low at around 200 μm. In this paper, we first present a hybrid mechanism of electrohydrodynamic jet printing with the FDM technique, which we name E-FDM. We then develop a novel high-resolution 3D printer based on the E-FDM process. To determine the optimal condition for structuring, we also investigated the effect of several printing parameters, such as temperature, applied voltage, working height, printing speed, flow-rate, and acceleration on the patterning results. This method was capable of fabricating both high resolution 2D and 3D structures with the use of polylactic acid (PLA). PLA has been used to fabricate scaffold structures for tissue engineering, which has different hierarchical structure sizes. The fabrication speed was up to 40 mm/s and the pattern resolution could be improved to 10 μm.

  5. Real-time hybrid simulation technique for performance evaluation of full-scale sloshing dampers in wind turbines

    Science.gov (United States)

    Zhang, Zili; Basu, Biswajit; Nielsen, Saren R. K.

    2016-09-01

    As a variation of the pseudodynamic testing technique, the real-time hybrid simulation (RTHS) technique is executed in real time, thus allowing investigation of structural systems with rate-dependent components. In this paper, the RTHS is employed for performance evaluation of full-scale liquid sloshing dampers in multi-megawatt wind turbines, where the tuned liquid damper (TLD) is manufactured and tested as the physical substructure while the wind turbine is treated as the numerical substructure and modelled in the computer using a 13-degree-of-freedom (13-DOF) aeroelastic model. Wind turbines with 2 MW and 3 MW capacities have been considered under various turbulent wind conditions. Extensive parametric studies have been performed on the TLD, e.g., various tuning ratios by changing the water level, TLD without and with damping screens (various mesh sizes of the screen considered), and TLD with flat and sloped bottoms. The present study provides useful guidelines for employing sloshing dampers in large wind turbines, and indicates huge potentials of applying RTHS technique in the area of wind energy.

  6. Screening for cardiac HERG potassium channel interacting proteins using the yeast two-hybrid technique.

    Science.gov (United States)

    Ma, Qingyan; Yu, Hong; Lin, Jijin; Sun, Yifan; Shen, Xinyuan; Ren, Li

    2014-02-01

    The human ERG protein (HERG or Kv 11.1) encoded by the human ether-a-go-go-related gene (herg) is the pore-forming subunit of the cardiac delayed rectifier potassium current (IKr) responsible for action potential (AP) repolarization. Mutations in HERG lead to long-QT syndrome, a major cause of arrhythmias. Protein-protein interactions are fundamental for ion channel trafficking, membrane localization, and functional modulation. To identify proteins involved in the regulation of the HERG channel, we conducted a yeast two-hybrid screen of a human heart cDNA library using the C-terminus or N-terminus of HERG as bait. Fifteen proteins were identified as HERG amino terminal (HERG-NT)-interacting proteins, including Caveolin-1 (a membrane scaffold protein with multiple interacting partners, including G-proteins, kinases and NOS), the zinc finger protein, FHL2 and PTPN12 (a non-receptor tyrosine phosphatase). Eight HERG carboxylic terminal (HERG-CT)-interacting proteins were also identified, including the NF-κB-interacting protein myotrophin, We have identified multiple potential interacting proteins that may regulate cardiac IKr through cytoskeletal interactions, G-protein modulation, phosphorylation and downstream second messenger and transcription cascades. These findings provide further insight into dynamic modulation of HERG under physiological conditions and arrhythmogenesis.

  7. Speeding-up the hybrid video watermarking techniques in the DWT domain

    Science.gov (United States)

    Chammem, A.; Mitrea, M.; Preteux, F.

    2010-01-01

    The watermarking state of the art exhibits the hybrid methods combining spread spectrum and side information principles. The present study is focussed on speeding up such an algorithm (jointly patented by SFR - Vodafone Group and Institut Telecom). The dead lock on the reference method is first identified: the embedding module accounts for 90% of the whole watermarking chain and that more than 99% of this time is spent on applying an attack procedure (required in order to grant a good robustness to this method). The main issue of the present study is to deploy Monte Carlo generators accurately representing the watermarking attacks. In this respect, two difficulties should be overcome. First, accurate statistical models for the watermarking attacks should be obtained. Secondly, efficient Monte Carlo simulators should be deployed for these models. The last part of the study was devoted to the experimental validations. The mark is inserted in the (9,7) DWT representation of video sequence. Several types of attacks have been considered (linear and non-linear filters, geometrical transformations, ...). The quantitative results proved that the data payload, transparency and robustness properties have been inherited from the reference method. However, the watermarking speed was increased by a factor of 80.

  8. Entrapment of glucoamylase by sol-gel technique in PhTES/TEOS hybrid matrixes

    Directory of Open Access Journals (Sweden)

    B. Vlad-Oros

    2007-12-01

    Full Text Available Mesoporous silica particles were prepared by the sol-gel method from different alkoxysilane precursors and used as a host matrix for encapsulation of glucoamylase, an enzyme widely used in fermentative industry. The aim was to investigate the physico-chemical properties of the different silica powders and their effect on the enzyme kinetics. The encapsulated enzymes followed Michaelis-Menten kinetics. The Michaelis constant (KM and the maximum rate of starch hydrolysis reaction (Vmax were calculated according to the Michaelis-Menten and Lineweaver-Burke plots. The values of the Michaelis constant (KM of the encapsulated enzymes were higher than those of the free enzyme. The temperature and pH infl uence on the activity of free and immobilized glucoamylase were also compared. The results of this study show that the enzymes immobilized in organic/inorganic hybrid silica matrixes (obtained by the sol-gel method, allowing the entrapped glucoamylase to retain its biological activity, are suitable for many different applications, (medicinal, clinical, analytical.

  9. Prediction of peak ground acceleration of Iran's tectonic regions using a hybrid soft computing technique

    Directory of Open Access Journals (Sweden)

    Mostafa Gandomi

    2016-01-01

    Full Text Available A new model is derived to predict the peak ground acceleration (PGA utilizing a hybrid method coupling artificial neural network (ANN and simulated annealing (SA, called SA-ANN. The proposed model relates PGA to earthquake source to site distance, earthquake magnitude, average shear-wave velocity, faulting mechanisms, and focal depth. A database of strong ground-motion recordings of 36 earthquakes, which happened in Iran's tectonic regions, is used to establish the model. For more validity verification, the SA-ANN model is employed to predict the PGA of a part of the database beyond the training data domain. The proposed SA-ANN model is compared with the simple ANN in addition to 10 well-known models proposed in the literature. The proposed model performance is superior to the single ANN and other existing attenuation models. The SA-ANN model is highly correlated to the actual records (R = 0.835 and ρ = 0.0908 and it is subsequently converted into a tractable design equation.

  10. Fast hybrid CPU- and GPU-based CT reconstruction algorithm using air skipping technique.

    Science.gov (United States)

    Lee, Byeonghun; Lee, Ho; Shin, Yeong Gil

    2010-01-01

    This paper presents a fast hybrid CPU- and GPU-based CT reconstruction algorithm to reduce the amount of back-projection operation using air skipping involving polygon clipping. The algorithm easily and rapidly selects air areas that have significantly higher contrast in each projection image by applying K-means clustering method on CPU, and then generates boundary tables for verifying valid region using segmented air areas. Based on these boundary tables of each projection image, clipped polygon that indicates active region when back-projection operation is performed on GPU is determined on each volume slice. This polygon clipping process makes it possible to use smaller number of voxels to be back-projected, which leads to a faster GPU-based reconstruction method. This approach has been applied to a clinical data set and Shepp-Logan phantom data sets having various ratio of air region for quantitative and qualitative comparison and analysis of our and conventional GPU-based reconstruction methods. The algorithm has been proved to reduce computational time to half without losing any diagnostic information, compared to conventional GPU-based approaches.

  11. A novel reconstruction method for giant incisional hernia: Hybrid laparoscopic technique

    Directory of Open Access Journals (Sweden)

    G Ozturk

    2015-01-01

    Full Text Available Background and Objectives: Laparoscopic reconstruction of ventral hernia is a popular technique today. Patients with large defects have various difficulties of laparoscopic approach. In this study, we aimed to present a new reconstruction technique that combines laparoscopic and open approach in giant incisional hernias. Materials and Methods: Between January 2006 and August 2012, 28 patients who were operated consequently for incisional hernia with defect size over 10 cm included in this study and separated into two groups. Group 1 (n = 12 identifies patients operated with standard laparoscopic approach, whereas group 2 (n = 16 labels laparoscopic technique combined with open approach. Patients were evaluated in terms of age, gender, body mass index (BMI, mean operation time, length of hospital stay, surgical site infection (SSI and recurrence rate. Results: There are 12 patients in group 1 and 16 patients in group 2. Mean length of hospital stay and SSI rates are similar in both groups. Postoperative seroma formation was observed in six patients for group 1 and in only 1 patient for group 2. Group 1 had 1 patient who suffered from recurrence where group 2 had no recurrence. Discussion: Laparoscopic technique combined with open approach may safely be used as an alternative method for reconstruction of giant incisional hernias.

  12. Hybrid Decompression and Fixation Technique Versus Plated 3-Vertebra Corpectomy for 4-Segment Cervical Myelopathy: Analysis of 81 Cases With a Minimum 2-Year Follow-Up.

    Science.gov (United States)

    Odate, Seiichi; Shikata, Jitsuhiko; Kimura, Hiroaki; Soeda, Tsunemitsu

    2016-07-01

    A retrospective comparative study. The purpose of this study was to compare the stability and outcomes of a hybrid technique with those of a 3-vertebra corpectomy in the management of 4-segment cervical myelopathy. Patients with primarily ventral disease and loss of cervical lordosis are considered good candidates for anterior surgery. Cervical corpectomy is commonly performed in patients with multilevel cervical myelopathy. Corpectomies including >3 vertebraes entail an extremely high risk of reconstruction failure. To avoid the need to perform a 3-vertebra corpectomy, we use a hybrid decompression and fixation technique. This hybrid technique is a technique to obtain optimum decompression and fixation in patients with multilevel cervical myelopathy. A total of 81 patients with multilevel cervical myelopathy who underwent 4-segment cervical fixation with a minimum 2-year follow-up were included. The hybrid technique involved combining a plated 2-vertebra corpectomy and single-level discectomy with stand-alone cage fixation. This technique was performed in 39 patients, and the plated 3-vertebra corpectomy was performed in 42 patients. Nine patients (21%) who underwent the plated 3-vertebra corpectomy were treated with halo immobilization, but no patient in the hybrid group required this treatment (P=0.002). There were fewer instances of reconstruction failure in the hybrid group than in the 3-vertebra corpectomy group (0% vs. 10%, respectively; P=0.048) and fewer instances of C5 palsy (3% vs. 17%, respectively; P vertebra corpectomy for 4-segment cervical fixation: a shorter graft bone and plate are required; the fixed segment has greater initial stability; postoperative external immobilization is simplified; and the risk of reconstruction failure and postoperative C5 palsy is reduced markedly.

  13. Hybrid microelectronic technology

    Science.gov (United States)

    Moran, P.

    Various areas of hybrid microelectronic technology are discussed. The topics addressed include: basic thick film processing, thick film pastes and substrates, add-on components and attachment methods, thin film processing, and design of thick film hybrid circuits. Also considered are: packaging hybrid circuits, automating the production of hybrid circuits, application of hybrid techniques, customer's view of hybrid technology, and quality control and assurance in hybrid circuit production.

  14. Hybrid dynamic radioactive particle tracking (RPT) calibration technique for multiphase flow systems

    Science.gov (United States)

    Khane, Vaibhav; Al-Dahhan, Muthanna H.

    2017-04-01

    The radioactive particle tracking (RPT) technique has been utilized to measure three-dimensional hydrodynamic parameters for multiphase flow systems. An analytical solution to the inverse problem of the RPT technique, i.e. finding the instantaneous tracer positions based upon instantaneous counts received in the detectors, is not possible. Therefore, a calibration to obtain a counts-distance map is needed. There are major shortcomings in the conventional RPT calibration method due to which it has limited applicability in practical applications. In this work, the design and development of a novel dynamic RPT calibration technique are carried out to overcome the shortcomings of the conventional RPT calibration method. The dynamic RPT calibration technique has been implemented around a test reactor with 1foot in diameter and 1 foot in height using Cobalt-60 as an isotopes tracer particle. Two sets of experiments have been carried out to test the capability of novel dynamic RPT calibration. In the first set of experiments, a manual calibration apparatus has been used to hold a tracer particle at known static locations. In the second set of experiments, the tracer particle was moved vertically downwards along a straight line path in a controlled manner. The obtained reconstruction results about the tracer particle position were compared with the actual known position and the reconstruction errors were estimated. The obtained results revealed that the dynamic RPT calibration technique is capable of identifying tracer particle positions with a reconstruction error between 1 to 5.9 mm for the conditions studied which could be improved depending on various factors outlined here.

  15. A technique for estimating seed production of common moist-soil plants - Fish and Wildlife Leaflet 13.4.5

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — Contains methods to estimate seed production of moist-soil plants inlcuding survey design - Leaflet is part of the Wetland Management Book series.

  16. Influence of tides and winds on fishing techniques and strategies in the Mamanguape River Estuary, Paraíba State, NE Brazil.

    Science.gov (United States)

    Bezerra, Dandara M M; Nascimento, Douglas M; Ferreira, Emmanoela N; Rocha, Pollyana D; Mourão, José S

    2012-09-01

    This work was carried out in two small fishing communities, Barra de Mamanguape and Tramataia, Northeastern Brazil. The aim was to study these traditional fishermen's knowledge and perception about tide and wind classifications, as well as their fishing strategies and techniques. Our research methodology involved various techniques: free interviews and semi-structured ones, guided tours and direct observations. The results obtained show the fishermen's classification of the tides according to the phases of the moon: 'breaking tide', 'flushing tide', 'dead tide' and 'big tide' designated technically these last as neap tide and spring tide, respectively. Wind is also an essential factor for the fishermen to make successful catches, and they classify it according to direction: North, South, East, Southeast, Southwest, Northeast and Northwest. The data show that fishermen's knowledge can also be useful in devising plans for management and conservation studies for this estuary.

  17. Technological exploration of BAC-FISH on mitotic chromosomes of maize

    Institute of Scientific and Technical Information of China (English)

    Yongsheng TAO; Zuxin ZHANG; Yonglin CHEN; Lijia LI; Yonglian ZHENG

    2008-01-01

    The rice BAC-DNA was used as probes and fluorescence in situ hybridization (FISH) was applied to the interphase and metaphase mitotic chromosomes of maize. To optimize the BAC-FISH technique, we respect-ively assayed the effect of several factors, including maize or rice genomic Cot DNA used as blocking reagent of DNA, washing temperatures and FAD concentration in the washing buffer and in the hybrid solution. The results show that Cot DNA of maize genome blocked the repet-itive sequence of the rice BAC-DNA when the Cot value was below 50. Meanwhile, it was necessary to adjust the Cot value according to the different probes and their ratios. Decreasing the concentration of FAD in the hybridization mixtures, adjusting the washing rate after hybridization, and most especially, blocking the rice-specific repetitive sequences of BAC-DNA could improve the positive signals of BAC-FISH.

  18. Establishment of Relationships between Material Design and Product Design Domains by Hybrid FEM-ANN Technique

    CERN Document Server

    Prakash, K Soorya; Raj, M Joseph Malvin

    2010-01-01

    In this paper, research on AI based modeling technique to optimize development of new alloys with necessitated improvements in properties and chemical mixture over existing alloys as per functional requirements of product is done. The current research work novels AI in lieu of predictions to establish association between material and product customary. Advanced computational simulation techniques like CFD, FEA interrogations are made viable to authenticate product dynamics in context to experimental investigations. Accordingly, the current research is focused towards binding relationships between material design and product design domains. The input to feed forward back propagation prediction network model constitutes of material design features. Parameters relevant to product design strategies are furnished as target outputs. The outcomes of ANN shows good sign of correlation between material and product design domains. The study enriches a new path to illustrate material factors at the time of new product d...

  19. On Potentials and Limitations of a Hybrid WLAN-RFID Indoor Positioning Technique

    Directory of Open Access Journals (Sweden)

    Silverio C. Spinella

    2010-01-01

    Full Text Available This paper addresses the important issue of position estimation in indoor environments. Starting point of the research is positioning techniques that exploit the knowledge of power levels of RF signals from multiple 802.11 WLAN APs (Access Points. In particular, the key idea in this paper is to enhance the performance of a WLAN fingerprinting approach by coupling it to a RFID-based procedure. WLAN and RFID technologies are synergistically used to provide a platform for a more performing positioning process, in which the very strong identification capabilities of the RFID technology allow to increase the accuracy of positioning systems via WLAN fingerprinting. The algorithm performance is assessed through general and repeatable experimental campaigns, during which the main algorithm parameters are dimensioned. The results testify both to the feasibility of the solution and to its higher accuracy (attainable at very reduced costs compared to traditional positioning techniques.

  20. Evaluation and Optimization Study on a Hybrid EOR Technique Named as Chemical-Alternating-Foam Floods

    Directory of Open Access Journals (Sweden)

    Xu Xingguang

    2017-01-01

    Full Text Available This work presents a novel Enhanced Oil Recovery (EOR method called Chemical-Alternating-Foam (CAF floods in order to overcome the drawbacks of the conventional foam flooding such as insufficient amount of in-situ foams, severe foam collapse and surfactant retention. The first part of this research focused on the comparison of conventional foam floods and CAF floods both of which had the same amount of gas and chemicals. It showed that: (1 CAF floods possessed the much greater Residual Resistance Factor (RRF at elevated temperature; (2 the accumulative oil recovery of the CAF floods was 10%-15% higher than that of the conventional foam flooding. After 1.8 Pore Volume (PV injection, the oil recovery reached the plateau for both methods; (3 CAF floods yielded the most amount of incremental oil at the 98% water cut (water content in the effluent, while the continuous foam floods achieved the best performance at 60% water cut. The second part of this work determined the optimal foam quality (gas/liquid ratio or the volume percent gas within foam, chemical/foam slug size ratio, cycle number and injection sequence for the CAF floods. It was found that the CAF was endowed with the peak performance if the foam quality, chemical/foam slug size ratio, cycle number was fixed at 80%, 1:1 and 3 respectively with the chemical slug being introduced ahead of the foam slug. Through systematic and thorough research, the proposed hybrid process has been approved to be a viable and effective method significantly strengthening the conventional foam flooding.

  1. High process yield rates of thermoplastic nanofluidic devices using a hybrid thermal assembly technique.

    Science.gov (United States)

    Uba, Franklin I; Hu, Bo; Weerakoon-Ratnayake, Kumuditha; Oliver-Calixte, Nyote; Soper, Steven A

    2015-02-21

    Over the past decade, thermoplastics have been used as alternative substrates to glass and Si for microfluidic devices because of the diverse and robust fabrication protocols available for thermoplastics that can generate high production rates of the desired structures at low cost and with high replication fidelity, the extensive array of physiochemical properties they possess, and the simple surface activation strategies that can be employed to tune their surface chemistry appropriate for the intended application. While the advantages of polymer microfluidics are currently being realized, the evolution of thermoplastic-based nanofluidic devices is fraught with challenges. One challenge is assembly of the device, which consists of sealing a cover plate to the patterned fluidic substrate. Typically, channel collapse or substrate dissolution occurs during assembly making the device inoperable resulting in low process yield rates. In this work, we report a low temperature hybrid assembly approach for the generation of functional thermoplastic nanofluidic devices with high process yield rates (>90%) and with a short total assembly time (16 min). The approach involves thermally sealing a high T(g) (glass transition temperature) substrate containing the nanofluidic structures to a cover plate possessing a lower T(g). Nanofluidic devices with critical feature sizes ranging between 25-250 nm were fabricated in a thermoplastic substrate (T(g) = 104 °C) and sealed with a cover plate (T(g) = 75 °C) at a temperature significantly below the T(g) of the substrate. Results obtained from sealing tests revealed that the integrity of the nanochannels remained intact after assembly and devices were useful for fluorescence imaging at high signal-to-noise ratios. The functionality of the assembled devices was demonstrated by studying the stretching and translocation dynamics of dsDNA in the enclosed thermoplastic nanofluidic channels.

  2. Development of a wireless bridge monitoring system for condition assessment using hybrid techniques

    Science.gov (United States)

    Whelan, Matthew J.; Fuchs, Michael P.; Gangone, Michael V.; Janoyan, Kerop D.

    2007-04-01

    The introduction and development of wireless sensor network technology has resulted in rapid growth within the field of structural health monitoring (SHM), as the dramatic cable costs associated with instrumentation of large civil structures is potentially alleviated. Traditionally, condition assessment of bridge structures is accomplished through the use of either vibration measurements or strain sensing. One approach is through quantifying dynamic characteristics and mode shapes developed through the use of relatively dense arrays of accelerometers. Another widely utilized method of condition assessment is bridge load rating, which is enabled through the use of strain sensors. The Wireless Sensor Solution (WSS) developed specifically for diagnostic bridge monitoring provides a hybrid system that interfaces with both accelerometers and strain sensors to facilitate vibration-based bridge evaluation as well as load rating and static analysis on a universal platform. This paper presents the development and testing of a wireless bridge monitoring system designed within the Laboratory for Intelligent Infrastructure and Transportation Technologies (LIITT) at Clarkson University. The system interfaces with low-cost MEMS accelerometers using custom signal conditioning for amplification and filtering tailored to the spectrum of typical bridge vibrations, specifically from ambient excitation. Additionally, a signal conditioning and high resolution ADC interface is provided for strain gauge sensors. To permit compensation for the influence of temperature, thermistor-based temperature sensing is also enabled. In addition to the hardware description, this paper presents features of the software applications and host interface developed for flexible, user-friendly in-network control of and acquisition from the sensor nodes. The architecture of the software radio protocol is also discussed along with results of field deployments including relatively large-scale networks and

  3. Hybrid Machine Learning Technique for Forecasting Dhaka Stock Market Timing Decisions

    OpenAIRE

    Shipra Banik; Khodadad Khan, A. F. M.; Mohammad Anwer

    2014-01-01

    Forecasting stock market has been a difficult job for applied researchers owing to nature of facts which is very noisy and time varying. However, this hypothesis has been featured by several empirical experiential studies and a number of researchers have efficiently applied machine learning techniques to forecast stock market. This paper studied stock prediction for the use of investors. It is always true that investors typically obtain loss because of uncertain investment purposes and unsigh...

  4. A HYBRID TECHNIQUE FOR PAPR REDUCTION OF OFDM USING DHT PRECODING WITH PIECEWISE LINEAR COMPANDING

    Directory of Open Access Journals (Sweden)

    Thammana Ajay

    2016-06-01

    Full Text Available Orthogonal Frequency Division Multiplexing (OFDM is a fascinating approach for wireless communication applications which require huge amount of data rates. However, OFDM signal suffers from its large Peak-to-Average Power Ratio (PAPR, which results in significant distortion while passing through a nonlinear device, such as a transmitter high power amplifier (HPA. Due to this high PAPR, the complexity of HPA as well as DAC also increases. For the reduction of PAPR in OFDM many techniques are available. Among them companding is an attractive low complexity technique for the OFDM signal’s PAPR reduction. Recently, a piecewise linear companding technique is recommended aiming at minimizing companding distortion. In this paper, a collective piecewise linear companding approach with Discrete Hartley Transform (DHT method is expected to reduce peak-to-average of OFDM to a great extent. Simulation results shows that this new proposed method obtains significant PAPR reduction while maintaining improved performance in the Bit Error Rate (BER and Power Spectral Density (PSD compared to piecewise linear companding method.

  5. Effect of tannic acid-fish scale gelatin hydrolysate hybrid nanoparticles on intestinal barrier function and α-amylase activity.

    Science.gov (United States)

    Wu, Shao-Jung; Ho, Yi-Cheng; Jiang, Shun-Zhou; Mi, Fwu-Long

    2015-07-01

    Practical application of tannic acid is limited because it readily binds proteins to form insoluble aggregates. In this study, tannic acid was self-assembled with fish scale gelatin hydrolysates (FSGH) to form stable colloidal complex nanoparticles. The nanoparticles prepared from 4 mg ml(-1) tannic acid and 4 mg ml(-1) FSGH had a mean particle size of 260.8 ± 3.6 nm, and showed a positive zeta potential (20.4 ± 0.4 mV). The nanoparticles acted as effective nano-biochelators and free radical scavengers because they provided a large number of adsorption sites for interaction with heavy metal ions and scavenging free radicals. The maximum adsorption capacity for Cu(2+) ions was 123.5 mg g(-1) and EC50 of DPPH radical scavenging activity was 21.6 ± 1.2 μg ml(-1). Hydroxyl radical scavenging effects of the nanoparticles were investigated by electron spin resonance spectroscopy. The copper-chelating capacity and free radical scavenging activity of the nanoparticles were associated with their capacity to inhibit Cu(2+) ion-induced barrier impairment and hyperpermeability of Caco-2 intestinal epithelial tight junction (TJ). However, α-amylase inhibitory activity of the nanoparticles was significantly lower than that of free tannic acid. The results suggest that the nanoparticles can ameliorate Cu(2+) ion induced intestinal epithelial TJ dysfunction without severely inhibiting the activity of the digestive enzymes.

  6. 转基因鱼的安全性及检测技术%Safety and detection technique of transgenic fishes

    Institute of Scientific and Technical Information of China (English)

    李风铃; 江艳华; 姚琳; 王联珠; 翟毓秀

    2014-01-01

    转基因鱼是20世纪人类最重要的成就之一,对于鱼类育种具有重要意义,但一直以来转基因鱼的产业化进程并不顺利,究其根本原因在于人们对其安全性顾虑重重。因此,本文分别从食用和生态两个角度对转基因鱼的安全性进行了综述,分析了其可能存在的安全隐患以及近年来围绕此问题开展的相关研究。然后对转基因鱼常用的检测技术进行讨论,并针对其安全监控提出建议,最后,对转基因鱼下一步的产业化趋势进行了展望。%Transgenic fish is regarded as one of the most significant achievements in the 20th century, which is very important for fish breeding. However, the industrialization of transgenic fish is unfavorable, and its root reason lies in the people to its security concerns. Therefore, the food safety and ecological safety of transgenic fishes are introduced respectively, and the hidden trouble in security and related research in recent years are analyzed. Then the frequently-used detection techniques of transgenic fishes are dated in detail, and some advices about safety monitoring of transgenic fishes are given. Finally, the follow-up work is viewed in this review.

  7. A radiation hybrid map of the European sea bass (Dicentrarchus labrax) based on 1581 markers: Synteny analysis with model fish genomes.

    Science.gov (United States)

    Guyon, Richard; Senger, Fabrice; Rakotomanga, Michaelle; Sadequi, Naoual; Volckaert, Filip A M; Hitte, Christophe; Galibert, Francis

    2010-10-01

    The selective breeding of fish for aquaculture purposes requires the understanding of the genetic basis of traits such as growth, behaviour, resistance to pathogens and sex determinism. Access to well-developed genomic resources is a prerequisite to improve the knowledge of these traits. Having this aim in mind, a radiation hybrid (RH) panel of European sea bass (Dicentrarchus labrax) was constructed from splenocytes irradiated at 3000 rad, allowing the construction of a 1581 marker RH map. A total of 1440 gene markers providing ~4400 anchors with the genomes of three-spined stickleback, medaka, pufferfish and zebrafish, helped establish synteny relationships with these model species. The identification of Conserved Segments Ordered (CSO) between sea bass and model species allows the anticipation of the position of any sea bass gene from its location in model genomes. Synteny relationships between sea bass and gilthead seabream were addressed by mapping 37 orthologous markers. The sea bass genetic linkage map was integrated in the RH map through the mapping of 141 microsatellites. We are thus able to present the first complete gene map of sea bass. It will facilitate linkage studies and the identification of candidate genes and Quantitative Trait Loci (QTL). The RH map further positions sea bass as a genetic and evolutionary model of Perciformes and supports their ongoing aquaculture expansion.

  8. Well-defined functional mesoporous silica/polymer hybrids prepared by an ICAR ATRP technique integrated with bio-inspired polydopamine chemistry for lithium isotope separation.

    Science.gov (United States)

    Liu, Yuekun; Liu, Xuegang; Ye, Gang; Song, Yang; Liu, Fei; Huo, Xiaomei; Chen, Jing

    2017-05-09

    Mesoporous silica/polymer hybrids with well-preserved mesoporosity were prepared by integrating the initiators for continuous activator regeneration (ICAR) atom transfer radical polymerization (ATRP) technique with the bio-inspired polydopamine (PDA) chemistry. By manipulating the auto-oxidative polymerization of dopamine, uniform PDA layers were deposited on the surfaces and pore walls of ordered mesoporous silicas (OMSs), thereby promoting the immobilization of ATRP initiators. Poly(glycidyl methacrylate) (PGMA) brushes were then grown from the OMSs by using the ICAR ATRP technique. The evolution of the mesoporous silica/polymer hybrids during synthesis, in terms of morphology, structure, surface and porous properties, was detailed. And, parameters influencing the controlled growth of polymer chains in the ICAR ATRP system were studied. Taking advantage of the abundant epoxy groups in the PGMA platform, post-functionalization of the mesoporous silica/polymer hybrids by the covalent attachment of macrocyclic ligands for the adsorptive separation of lithium isotopes was realized. Adsorption behavior of the functionalized hybrids toward lithium ions was fully investigated, highlighting the good selectivity, and effects of temperature, solvent and counter ions. The ability for lithium isotope separation was evaluated. A higher separation factor could be obtained in systems with softer counter anions and lower polarity solvents. More importantly, due to the versatility of the ICAR ATRP technique, combined with the non-surface specific PDA chemistry, the methodology established in this work would provide new opportunities for the preparation of advanced organic-inorganic porous hybrids for broadened applications.

  9. A sixth order hybrid finite difference scheme based on the minimized dispersion and controllable dissipation technique

    Science.gov (United States)

    Sun, Zhen-sheng; Luo, Lei; Ren, Yu-xin; Zhang, Shi-ying

    2014-08-01

    The dispersion and dissipation properties of a scheme are of great importance for the simulation of flow fields which involve a broad range of length scales. In order to improve the spectral properties of the finite difference scheme, the authors have previously proposed the idea of optimizing the dispersion and dissipation properties separately and a fourth order scheme based on the minimized dispersion and controllable dissipation (MDCD) technique is thus constructed [29]. In the present paper, we further investigate this technique and extend it to a sixth order finite difference scheme to solve the Euler and Navier-Stokes equations. The dispersion properties of the scheme is firstly optimized by minimizing an elaborately designed integrated error function. Then the dispersion-dissipation condition which is newly derived by Hu and Adams [30] is introduced to supply sufficient dissipation to damp the unresolved wavenumbers. Furthermore, the optimized scheme is blended with an optimized Weighted Essentially Non-Oscillation (WENO) scheme to make it possible for the discontinuity-capturing. In this process, the approximation-dispersion-relation (ADR) approach is employed to optimize the spectral properties of the nonlinear scheme to yield the true wave propagation behavior of the finite difference scheme. Several benchmark test problems, which include broadband fluctuations and strong shock waves, are solved to validate the high-resolution, the good discontinuity-capturing capability and the high-efficiency of the proposed scheme.

  10. A Hybrid Approach for Detecting Suspicious Accounts in Money Laundering Using Data Mining Techniques

    Directory of Open Access Journals (Sweden)

    Ch. Suresh

    2016-05-01

    Full Text Available Money laundering is a criminal activity to disguise black money as white money. It is a process by which illegal funds and assets are converted into legitimate funds and assets. Money Laundering occurs in three stages: Placement, Layering, and Integration. It leads to various criminal activities like Political corruption, smuggling, financial frauds, etc. In India there is no successful Anti Money laundering techniques which are available. The Reserve Bank of India (RBI, has issued guidelines to identify the suspicious transactions and send it to Financial Intelligence Unit (FIU. FIU verifies if the transaction is actually suspicious or not. This process is time consuming and not suitable to identify the illegal transactions that occurs in the system. To overcome this problem we propose an efficient Anti Money Laundering technique which can able to identify the traversal path of the Laundered money using Hash based Association approach and successful in identifying agent and integrator in the layering stage of Money Laundering by Graph Theoretic Approach.

  11. Embryonic and genetic manipulation in fish

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    Fishes, the biggest and most diverse community in vertebrates are good experimental models for studies of cell and developmental biology by many favorable charac teristics. Nuclear transplantation in fish has been thor oughly studied in China since 1960s. Fish nuclei of em bryonic cells from different genera were transplanted into enucleated eggs generating nucleo-cytoplasmic hybrids of adults. Most importantly, nuclei of cultured goldfish kid ney cells had been reprogrammed in enucleated eggs to support embryogenesis and ontogenesis of a fertile fish. This was the first case of cloned fish with somatic cells. Based on the technique of microinjection, recombinant MThGH gene has been transferred into fish eggs and the first batch of transgenic fish were produced in 1984. The behavior of foreign gene was characterized and the onset of the foreign gene replication occurred between the blas tula to gastrula stages and random integration mainly oc curred at later stages of embryogenesis. This eventually led to the transgenlc mosaicism. The MThGH-transferred common carp enhanced growth rate by 2-4 times in the founder juveniles and doubled the body weight in the adults. The transgenic common carp were more efficient in utilizing dietary protein than the controls. An “all-fish” gene construct CAgcGH has been made by splicing the common carpβ-actin gene (CA) promoter onto the grass carp growth hormone gene (gcGH) coding sequence. The CAgcGH-transferred Yellow River Carp have also shown significantly fast-growth trait. Combination of techniques * Corresponding author, Fax: 0086-27-87876624 E-mail: zyzhu@ihb.ac.cn of fish cell culture, gene transformation with cultured cells and nuclear transplantation should be able to generate ho mogeneous strain of valuable transgenic fish to fulfil human requirement in 21st century.

  12. Development and validation of a hybrid simulation technique for cone beam CT: application to an oral imaging system

    Science.gov (United States)

    Zhang, G.; Pauwels, R.; Marshall, N.; Shaheen, E.; Nuyts, J.; Jacobs, R.; Bosmans, H.

    2011-09-01

    This paper proposes a hybrid technique to simulate the complete chain of an oral cone beam computed tomography (CBCT) system for the study of both radiation dose and image quality. The model was developed around a 3D Accuitomo 170 unit (J Morita, Japan) with a tube potential range of 60-90 kV. The Monte Carlo technique was adopted to simulate the x-ray generation, filtration and collimation. Exact dimensions of the bow-tie filter were estimated iteratively using experimentally acquired flood images. Non-flat radiation fields for different exposure settings were mediated via 'phase spaces'. Primary projection images were obtained by ray tracing at discrete energies and were fused according to the two-dimensional energy modulation templates derived from the phase space. Coarse Monte Carlo simulations were performed for scatter projections and the resulting noisy images were smoothed by Richardson-Lucy fitting. Resolution and noise characteristics of the flat panel detector were included using the measured modulation transfer function (MTF) and the noise power spectrum (NPS), respectively. The Monte Carlo dose calculation was calibrated in terms of kerma free-in-air about the isocenter, using an ionization chamber, and was subsequently validated by comparison against the measured air kerma in water at various positions of a cylindrical water phantom. The resulting dose discrepancies were found <10% for most cases. Intensity profiles of the experimentally acquired and simulated projection images of the water phantom showed comparable fractional increase over the common area as changing from a small to a large field of view, suggesting that the scatter was accurately accounted. Image validation was conducted using two small phantoms and the built-in quality assurance protocol of the system. The reconstructed simulated images showed high resemblance on contrast resolution, noise appearance and artifact pattern in comparison to experimentally acquired images, with <5

  13. A novel technique to neutralize the Yawing moment due to asymmetric thrust in a hybrid buoyant aircraft

    Science.gov (United States)

    Haque, Anwar U.; Asrar, Waqar; Omar, Ashraf A.; Sulaeman, Erwin; J. S Ali, Mohamed

    2016-03-01

    Dorsal fin is used in swimming animals like shark for the generation of thrust as well as to meet the requirement of the lateral stability. In the case of aircraft, rudders are normally used for the said requirement. In the present work, this nature inspired idea is explored for its application to neutralize the unavoidable asymmetric thrust produced by the twin engines of a hybrid buoyant aircraft. First, the estimation of asymmetric thrust is obtained with the help of analytical techniques for maximum thrust condition at 4 degree angle of attack. The moment generated by it is utilized for the sizing of a dorsal fin which looks similar to a tapered wing and is placed aft of the center of gravity. Wind tunnel testing at subsonic speed is carried out to explore the design features of this rotatable dorsal fin. It is found that a small rotation of 5 degree can generate the required moment. However, such rotation requires a complete pneumatic/electro-mechanical system and an alternative of it is to use a cambered airfoil for the dorsal fin installed at fixed location. Such a flow controlling device can also be used as an antenna mast, which is commonly installed out the fuselage of the aircraft for communication purposes. Moreover, by incorporating this technique, a pilot doesn't have to put an extra effort to make the aircraft stable in the presence of side wind.

  14. Research on Theories and Techniques of Irrigation for Safeguarding Seed Production of Two-Line Hybrid Rice

    Institute of Scientific and Technical Information of China (English)

    ZOU Jiang-shi; L(U) Chuan-gen; YAO Ke-min; HU Ning; XIA Shi-jian

    2006-01-01

    By inducing frequency, intensity and duration of lower temperature in the middle and last ten-day periods in August in the rice-growing areas of southern China, increasing temperature for safe seed production was defined as 2℃. During the sensitive period of fertility, characters of panicle height and canopy structure of TGMS rice, Pei'ai64S, were measured.Results showed that temperature changes caused by irrigation in fields were below 40 cm of rice plant, and heating effect was significant at 20 cm when the temperature was increased by 3.1℃. For the present study, the following irrigation techniques were put forth: the water depth of 15-20 cm, current water used, irrigating after 17:00 and bailing at 10:00 in sunny or cloudy weather, irrigating on whole day, in shady or rainy weather, increasing inflows and outflows in large fields. In the present experiment, pollen fertility and self-fertilized seed setting rate accepted that the techniques were feasible and effective for against lower temperature and safeguarding seed production of two-line hybrid rice.

  15. A novel technique to neutralize the Yawing moment due to asymmetric thrust in a hybrid buoyant aircraft

    Directory of Open Access Journals (Sweden)

    Haque Anwar U

    2016-01-01

    Full Text Available Dorsal fin is used in swimming animals like shark for the generation of thrust as well as to meet the requirement of the lateral stability. In the case of aircraft, rudders are normally used for the said requirement. In the present work, this nature inspired idea is explored for its application to neutralize the unavoidable asymmetric thrust produced by the twin engines of a hybrid buoyant aircraft. First, the estimation of asymmetric thrust is obtained with the help of analytical techniques for maximum thrust condition at 4 degree angle of attack. The moment generated by it is utilized for the sizing of a dorsal fin which looks similar to a tapered wing and is placed aft of the center of gravity. Wind tunnel testing at subsonic speed is carried out to explore the design features of this rotatable dorsal fin. It is found that a small rotation of 5 degree can generate the required moment. However, such rotation requires a complete pneumatic/electro-mechanical system and an alternative of it is to use a cambered airfoil for the dorsal fin installed at fixed location. Such a flow controlling device can also be used as an antenna mast, which is commonly installed out the fuselage of the aircraft for communication purposes. Moreover, by incorporating this technique, a pilot doesn’t have to put an extra effort to make the aircraft stable in the presence of side wind.

  16. Optimizing an Industrial Scale Naphtha Catalytic Reforming Plant Using a Hybrid Artificial Neural Network and Genetic Algorithm Technique

    Directory of Open Access Journals (Sweden)

    Sepehr Sadighi

    2015-07-01

    Full Text Available In this paper, a hybrid model for estimating the activity of a commercial Pt-Re/Al2O3 catalyst in an industrial scale heavy naphtha catalytic-reforming unit (CRU is presented. This model is also capable of predicting research octane number (RON and yield of gasoline. In the proposed model, called DANN, the decay function of heterogeneous catalysts is combined with a recurrent-layer artificial neural network. During a life cycle (919 days, fifty-eight points are selected for building and training the DANN (60%, nineteen data points for testing (20%, and the remained ones for validating steps. Results show that DANN can acceptably estimate the activity of catalyst during its life in consideration of all process variables. Moreover, it is confirmed that the proposed model is capable of predicting RON and yield of gasoline for unseen (validating data with AAD% (average absolute deviation of 0.272% and 0.755%, respectively. After validating the model, the octane barrel level (OCB of the plant is maximized by manipulating the inlet temperature of reactors, and hydrogen to hydrocarbon molar ratio whilst all process limitations are taken into account. During a complete life cycle results show that the decision variables, generated by the optimization program, can increase the RON, process yield and OCB of CRU to about 1.15%, 3.21%, and 4.56%, respectively. © 2015 BCREC UNDIP. All rights reserved.Received: 27th July 2014; Revised: 31st May 2015; Accepted: 31th May 2015 How to Cite: Sadighi, S., Mohaddecy, R.S., Norouzian, A. (2015. Optimizing an Industrial Scale Naphtha Catalytic Reforming Plant Using a Hybrid Artificial Neural Network and Genetic Algorithm Technique. Bulletin of Chemical Reaction Engineering & Catalysis, 10(2: 210-220. (doi:10.9767/bcrec.10.2.7171.210-220 Permalink/DOI: http://dx.doi.org/10.9767/bcrec.10.2.7171.210-220  

  17. 全染色体涂染探针FISH技术在AIH产前诊断唐氏综合征的应用%Application of FISH technique with whole chromosome probe for prenatal diagnosis of Downs syndrome after AIH

    Institute of Scientific and Technical Information of China (English)

    刘新雄; 梁荣伟; 符可鹏; 陈哲; 孙雷; 翁勋锦

    2012-01-01

    Objective; To explore the application value of fluorescence in situ hybridization ( FISH) by using self - designed human whole chromosome 21 special DNA probe for prenatal diagnosis of Downs syndrome after artificial insemination by husband (AIH) . Methods; FISH of uncultured amniotic fluid cells abstracted from pregnant women of 16 - 26 gestational weeks after AIH treatment was performed with self - designed human whole chromosome 21 special DNA probe, routine cell culture and chromosomal karyotype analysis were conducted at the same time, and the results of the two methods were compared. Results; The result of FISH was obtained within 24 hours, one child with trisomy 21 and one child with triple X syndrome were found. The coincidence rate of self - designed human whole chromosome 21 special DNA probe for detection of chromosome 21 in uncultured amniotic fluid cells was as high as 99.42% , no abnormal FISH result was found in the patients whose chromosomal karyotype was 47, XXX. The detection results were identical with the results of chromosomal karyotype analysis and follow - up. Conclusion; FISH technique with self - designed human whole chromosome 21 special DNA probe has the advantages of speediness and accuracy, which can quicken the diagnostic time, FISH technique has good application value for the high risk pregnant women after successful AIH in the prenatal diagnosis of Downs syndrome.%目的:探讨应用自制的人21号全染色体特异DNA涂染探针FISH技术在AIH产前诊断唐氏综合征的应用价值.方法:对经AIH治疗成功受孕的妊娠16 ~26周孕妇抽取的未培养羊水细胞采用已制备的人21号全染色体特异DNA涂染探针进行荧光原位杂交,同时进行常规细胞培养及染色体核型分析,并比较两种检测方法的结果.结果:自制探针FISH检测均于24h内出结果,检测出患儿2例,其中1例为标准21三体,1例为X三体.自制的人21号全染色体特异DNA涂染探针

  18. Recent advances in knowledge-based paradigms and applications enhanced applications using hybrid artificial intelligence techniques

    CERN Document Server

    Jain, Lakhmi

    2014-01-01

    This book presents carefully selected contributions devoted to the modern perspective of AI research and innovation. This collection covers several areas of applications and motivates new research directions. The theme across all chapters combines several domains of AI research , Computational Intelligence and Machine Intelligence including an introduction to  the recent research and models. Each of the subsequent chapters reveals leading edge research and innovative solution that employ AI techniques with an applied perspective. The problems include classification of spatial images, early smoke detection in outdoor space from video images, emergent segmentation from image analysis, intensity modification in images, multi-agent modeling and analysis of stress. They all are novel pieces of work and demonstrate how AI research contributes to solutions for difficult real world problems that benefit the research community, industry and society.

  19. Rapid full Mueller matrix imaging polarimetry based on the hybrid phase modulation technique

    Science.gov (United States)

    Han, Chien-Yuan; Du, Cheng-You; Jhou, Jhe-Yi

    2017-01-01

    In this work, we present a novel method of Mueller matrix imaging polarimetry, which comprises dual liquid crystal variable retarders at the polarization generation portion and a photoelastic modulator at the polarization analysis portion. The light source can be operated either in the continuous mode, which provides an in-situ calibration process for the liquid crystal variable retarders, or in the pulse mode to deduce the full two-dimensional Mueller matrix with 16 images from the camera. We measured the Mueller matrix images of air as a standard test, as well as a quarter wave plate to determine its azimuthal angle and phase retardation by the polar decomposition technique. Finally, the decomposed Mueller matrix images of a biopolymer specimen with the conformational change produced by heat treatment are presented.

  20. MULTILEVEL APPROACH OF CBIR TECHNIQUES FOR VEGETABLE CLASSIFICATION USING HYBRID IMAGE FEATURES

    Directory of Open Access Journals (Sweden)

    D. Latha

    2016-02-01

    Full Text Available CBIR is a technique to retrieve images semantically relevant to query image from an image database. The challenge in CBIR is to develop a method that should increase the retrieval accuracy and reduce the retrieval time. In order to improve the retrieval accuracy and runtime, a multilevel CBIR approach is proposed in this paper. In the first level, the color attributes like mean and standard deviations are proposed to calculate on HSV color space to retrieve the images with minimum disparity distance from the database. In order to minimize search area, in the second level Local Ternary Pattern is proposed on images which were selected from the first level. Experimental results and comparisons demonstrate the superiority of the proposed approach.

  1. A Hybrid Model for the Mid-Long Term Runoff Forecasting by Evolutionary Computaion Techniques

    Institute of Scientific and Technical Information of China (English)

    Zou Xiu-fen; Kang Li-shan; Cae Hong-qing; Wu Zhi-jian

    2003-01-01

    The mid-long term hydrology forecasting is one of most challenging problems in hydrological studies. This paper proposes an efficient dynamical system prediction model using evolutionary computation techniques. The new model overcomes some disadvantages of conventional hydrology fore casting ones. The observed data is divided into two parts: the slow "smooth and steady" data, and the fast "coarse and fluctuation" data. Under the divide and conquer strategy, the behavior of smooth data is modeled by ordinary differential equations based on evolutionary modeling, and that of the coarse data is modeled using gray correlative forecasting method. Our model is verified on the test data of the mid-long term hydrology forecast in tbe northeast region of China. The experimental results show that the model is superior to gray system prediction model (GSPM).

  2. Integrating Internet Video Conferencing Techniques and Online Delivery Systems with Hybrid Classes to Enhance Student Interaction and Learning in Accelerated Programs

    Science.gov (United States)

    Beckwith, E. George; Cunniff, Daniel T.

    2009-01-01

    Online course enrollment has increased dramatically over the past few years. The authors cite the reasons for this rapid growth and the opportunities open for enhancing teaching/learning techniques such as video conferencing and hybrid class combinations. The authors outlined an example of an accelerated learning, eight-class session course…

  3. Fabrication of 2×2 Thermo-Optic Switches with Organic-Inorganic Hybrid Materials Prepared by Sol-Gel Technique

    Institute of Scientific and Technical Information of China (English)

    2003-01-01

    2×2 Mach-Zehnder interferometric thermo-optic switch was fabricated with organic/inorganic hybrid materials by sol-gel technique and direct UV patterning. The switching time of device was measured to be 4.2 ms and switching power 9.3 mW.

  4. Contrast-Enhanced MR Angiography (CEMRA) in Peripheral Arterial Occlusive Disease (PAOD): conventional moving table technique versus hybrid technique; Kontrastverstaerkte MR-Angiographie (CEMRA) bei peripherer AVK (pAVK): konventionelle Tischverschiebetechnik versus Hybrid-Technik

    Energy Technology Data Exchange (ETDEWEB)

    Kalle, T. von; Gerlach, A.; Hatopp, A.; Klinger, S.; Prodehl, P.; Arlat, I.P. [Katharinenhospital, Stuttgart (Germany). Radiologisches Inst.

    2004-01-01

    Patients and Methods: 80 patients (males n = 60, females n = 20, median age = 70 years, diabetics n = 27) with PAOD were examined with a 1,5T system (40 mT/m) using a dedicated phased array peripheral vascular coil. Protocol A consisted of a single injection of Gd-BOPTA with consecutive craniocaudal image acquisition and protocol B of two injections, with the first injection of Gd-BOPTA followed by image acquisition of the popliteocrural and pedal segments and the second injection followed by acquiring the aortoiliac and femoral segments (hybrid technique). The evaluation of the arterial system was directed to the iliac, femoral, popliteocrural and pedal arteries. Results: The visualization of the entire aortopedal vascular system was of diagnostically good or satisfactory quality in 16 of 40 patients using protocol A and in 29 of 40 patients using protocol B (iliac 40 vs. 37, femoral 40 vs. 40, popliteocrural 35 vs. 37, pedal 16 vs. 29); without the pedal station the number increased to 35 of 40 patients for both protocols. The reason of diagnostic limitations was an arteriovenous overlap in 24 of 80 cases, with 19 of 40 cases for protocol A and 5 of 40 for protocol B, located exclusively in the cruropedal region. Conclusion: Moving table hybrid CEMRA is superior to conventional technique in craniocaudal direction by producing less venous overlap of arteries and is especially more suitable for the diagnostic evaluation of the cruropedal region. (orig.) [German] Patienten und Methodik: Untersucht wurden 80 Patienten (maennl. n = 60, weibl. n = 20, mittl. Alter 70 J., Diabetiker n = 27) mit pAVK an einem 1,5-Tesla-Geraet (40 mT/m) mit dedizierter Phased-Array-Oberflaechen-Gefaessspule. Protokoll A beinhaltete eine Kontrastmittel-Injektion (Gd-BOPTA) mit konsekutiver kraniokaudaler Bildakquisition. In Protokoll B erfolgte die Akquisition zunaechst der Unterschenkel- und Fussetage mittels einer ersten, anschliessend der Abdomen-Becken- und Oberschenkeletage mittels

  5. A hybrid stock trading framework integrating technical analysis with machine learning techniques

    Directory of Open Access Journals (Sweden)

    Rajashree Dash

    2016-03-01

    Full Text Available In this paper, a novel decision support system using a computational efficient functional link artificial neural network (CEFLANN and a set of rules is proposed to generate the trading decisions more effectively. Here the problem of stock trading decision prediction is articulated as a classification problem with three class values representing the buy, hold and sell signals. The CEFLANN network used in the decision support system produces a set of continuous trading signals within the range 0–1 by analyzing the nonlinear relationship exists between few popular technical indicators. Further the output trading signals are used to track the trend and to produce the trading decision based on that trend using some trading rules. The novelty of the approach is to engender the profitable stock trading decision points through integration of the learning ability of CEFLANN neural network with the technical analysis rules. For assessing the potential use of the proposed method, the model performance is also compared with some other machine learning techniques such as Support Vector Machine (SVM, Naive Bayesian model, K nearest neighbor model (KNN and Decision Tree (DT model.

  6. A Hybrid Technique for Real Time License Plate Localization with the aid of FFBPNNAPSO

    Directory of Open Access Journals (Sweden)

    Reji PI

    2016-04-01

    Full Text Available Vehicle License Plate Recognition (VLPR is an imperative constituent in Intelligent Transportation Systems (ITS, which encircles three foremost phases essentially License Plate Localization (LPL, Character Segmentation (CS, Character Recognition (CR. In this paper, we have intended to introduce a novel License Plate Localization algorithm subjected to Artificial Neural Networks (ANN. This proposed scheme involves distinct phases of pre-processing, image de-noising and enhancement, feature extraction, Neural Network training and License Plate detection. Followed by the mining of assorted statistical features, geometrical features, edge features and texture features from the vehicular image, they are given as the input to Feed Forward Back Propagation Neural Network (FFBPNN in order to localize the License Plate. During the training process, the parameters of the FFBPNN will be optimized using the eminent Adaptive Particle Swarm Optimization (APSO algorithm in order to improve the Neural Network convergence performance. The License Plate Localization of our proposed technique is analyzed with simple Feed Forward Back propagation Neural Network (FFBPNN in terms of accuracy, sensitivity and specificity. The experimental outcomes demonstrate that the proposed procedure proficiently accomplishes an extremely high localization rate with elevated specificity (91.3%.

  7. Flexible Riser Monitoring Using Hybrid Magnetic/Optical Strain Gage Techniques through RLS Adaptive Filtering

    Directory of Open Access Journals (Sweden)

    Pipa Daniel

    2010-01-01

    Full Text Available Flexible riser is a class of flexible pipes which is used to connect subsea pipelines to floating offshore installations, such as FPSOs (floating production/storage/off-loading unit and SS (semisubmersible platforms, in oil and gas production. Flexible risers are multilayered pipes typically comprising an inner flexible metal carcass surrounded by polymer layers and spiral wound steel ligaments, also referred to as armor wires. Since these armor wires are made of steel, their magnetic properties are sensitive to the stress they are subjected to. By measuring their magnetic properties in a nonintrusive manner, it is possible to compare the stress in the armor wires, thus allowing the identification of damaged ones. However, one encounters several sources of noise when measuring electromagnetic properties contactlessly, such as movement between specimen and probe, and magnetic noise. This paper describes the development of a new technique for automatic monitoring of armor layers of flexible risers. The proposed approach aims to minimize these current uncertainties by combining electromagnetic measurements with optical strain gage data through a recursive least squares (RLSs adaptive filter.

  8. Heart dose reduction in breast cancer treatment with simultaneous integrated boost. Comparison of treatment planning and dosimetry for a novel hybrid technique and 3D-CRT

    Energy Technology Data Exchange (ETDEWEB)

    Joest, Vincent; Kretschmer, Matthias; Sabatino, Marcello; Wuerschmidt, Florian; Dahle, Joerg; Lorenzen, Joern [Radiological Alliance, Hamburg (Germany); Ueberle, Friedrich [University of Applied Sciences, Faculty Life Sciences, Hamburg (Germany)

    2015-09-15

    The present study compares in silico treatment plans of clinically established three-dimensional conformal radiotherapy (3D-CRT) with a hybrid technique consisting of intensity-modulated radiotherapy (IMRT) and volumetric modulated arc radiotherapy (VMAT) during normally fractionated radiation of mammary carcinomas with simultaneous integrated boost on the basis of dose-volume histogram (DVH) parameters. Radiation treatment planning was performed with a hybrid and a 3D-CRT treatment plan for 20 patients. Hybrid plans were implemented with two tangential IMRT fields and a VMAT field in the angular range of the tangents. Verification of the plan was performed with a manufacturer-independent measurement system consisting of a detector array and rotation unit. The mean values of the heart dose for the entire patient collective were 3.6 ± 2.5 Gy for 3D-CRT and 2.9 ± 2.1 Gy for the hybrid technique (p < 0.01). For the left side (n = 10), the mean values for the left anterior descending artery were 21.8 ± 7.4 Gy for 3D-CRT and 17.6 ± 7.4 Gy for the hybrid technique (p < 0.01). The mean values of the ipsilateral lung were 11.9 ± 1.6 Gy for 3D-CRT and 10.5 ± 1.3 Gy for the hybrid technique (p < 0.01). Calculated dose distributions in the hybrid arm were in good accordance with measured dose (on average 95.6 ± 0.5 % for γ < 1 and 3 %/3 mm). The difference of the mean treatment time per fraction was 7 s in favor of 3D-CRT. Compared with the established 3D-CRT technique, the hybrid technique allows for a decrease in dose, particularly of the mean heart and lung dose with comparable target volume acquisition and without disadvantageous low-dose load of contralateral structures. Uncomplicated implementation of the hybrid technique was demonstrated in this context. The hybrid technique combines the advantages of tangential IMRT with the superior sparing of organs at risk by VMAT. (orig.) [German] Die vorliegende Studie vergleicht ''in silico

  9. Chitosan-pectin hybrid nanoparticles prepared by coating and blending techniques.

    Science.gov (United States)

    Rampino, A; Borgogna, M; Bellich, B; Blasi, P; Virgilio, F; Cesàro, A

    2016-03-10

    The preparation of chitosan nanoparticles in combination with pectins, as additional mucoadhesive biopolymers, was investigated. Pectins from apple and from citrus fruit were considered; polygalacturonic acid was taken as a reference. Tripolyphosphate was used as an anionic cross-linker. Two different techniques were compared, namely the coating and the blending. Coated nanoparticles (NPs) in the ratio pectin:NPs from 2:1 to 5:1 evidenced that the size of NPs increased as the amount of pectin (both from apple and citrus fruit) was increased. In particular, for NPs coated with pectin from citrus fruit the size ranges from 200 to 260nm; while for NPs coated with pectin from apple the size ranges from 330 to 450nm. A minimum value of Z-potential around -35mV was obtained for the ratio pectin:NPs 4:1, while further addition of pectin did not decrease the Z-potential. Also blended NPs showed a dependence of the size on the ratio of the components: for a given ratio pectin:tripolyphosphate the size increases as the fraction of chitosan increases; for a low ratio chitosan:pectin a high amount of tripolyphosphate was needed to obtain a compact structure. The effect of the additional presence of loaded proteins in chitosan-pectin nanoparticles was also investigated, since proteins contribute to alter the electrostatic interactions among charged species. FT-IR and DSC characterization are presented to confirm the interactions between biopolymers. Finally, the biocompatibility of the used materials was assessed by the chorioallantoic membrane assay, confirming the safety of the materials.

  10. SURVEY OF THE NOCUITÉ OF THE CONTRAPTIONS AND TECHNIQUES OF ARTISANAL FISHING ON THE POPULATIONS OF NAVY TURTLES OF THE BAY OF LOANGO (CONGO

    Directory of Open Access Journals (Sweden)

    Parisse Akouango

    2013-08-01

    Full Text Available Chelonia marine turtle species preservation is one of the international community challenges through the world. In Congo-Brazzaville, the Renatura organization (ONG is engage to the biodiversity marine turtle species protection. Among the five of seven marine turtle species encountered in the coast, Turtle lute (Dermochelys coriacea; Green turtle (Chelonia mydas; Olive turtle (Lepidochelys olivacea; Turtle caouanne (Caretta caretta and Turtle overlapped (Eretmochelys imbricata. All these species of navy turtles well that protected by different international conventions, undergo several threats among which the nets and contraptions of traditional fishing constitute one of the main reasons of their decline. Indeed the accidental captures in the nets of the traditional fishers represent a serious threat for the navy turtles. This problem is delicate to manage because these holds don't depend directly on the will of the fishers. Indeed the turtles take themselves inauspiciously in the nets and entangle themselves of it while trying to clear itself/themselves of the net. To remedy this situation, Renatura Congo has, in 2005, initiated a program of liberation of the accidental captures of navy turtles in the nets of traditional fishing. About 1500 liberations are done annually by the agents of Renatura Congo. The liberation of these turtles doesn't constitute a long-term solution. Also, before the size of their accidental captures, it appeared to us necessary to bring our contribution in the survey of the nocuité of the contraptions and techniques of traditional fishing used to the bay of Loango in order to meter in evidence those that are more harmful. Such an objective reaches once, will allow us to improve these contraptions and techniques in order to reduce the risks of accidental captures while maintaining the level of the holds of fish and other aquatic organisms.

  11. Hybrid approach combining multiple characterization techniques and simulations for microstructural analysis of proton exchange membrane fuel cell electrodes

    Science.gov (United States)

    Cetinbas, Firat C.; Ahluwalia, Rajesh K.; Kariuki, Nancy; De Andrade, Vincent; Fongalland, Dash; Smith, Linda; Sharman, Jonathan; Ferreira, Paulo; Rasouli, Somaye; Myers, Deborah J.

    2017-03-01

    The cost and performance of proton exchange membrane fuel cells strongly depend on the cathode electrode due to usage of expensive platinum (Pt) group metal catalyst and sluggish reaction kinetics. Development of low Pt content high performance cathodes requires comprehensive understanding of the electrode microstructure. In this study, a new approach is presented to characterize the detailed cathode electrode microstructure from nm to μm length scales by combining information from different experimental techniques. In this context, nano-scale X-ray computed tomography (nano-CT) is performed to extract the secondary pore space of the electrode. Transmission electron microscopy (TEM) is employed to determine primary C particle and Pt particle size distributions. X-ray scattering, with its ability to provide size distributions of orders of magnitude more particles than TEM, is used to confirm the TEM-determined size distributions. The number of primary pores that cannot be resolved by nano-CT is approximated using mercury intrusion porosimetry. An algorithm is developed to incorporate all these experimental data in one geometric representation. Upon validation of pore size distribution against gas adsorption and mercury intrusion porosimetry data, reconstructed ionomer size distribution is reported. In addition, transport related characteristics and effective properties are computed by performing simulations on the hybrid microstructure.

  12. Recovery and reuse of hexavalent chromium from aqueous solutions by a hybrid technique of electrodialysis and ion exchange

    Energy Technology Data Exchange (ETDEWEB)

    Gayathri, R. [Sengunthar Engineering College, Tiruchengode (India). Dept. of Civil Engineering], e-mail: gay3civil@gmail.com; Senthil Kumar, P. [SSN College of Engineering, Chennai (India). Dept. of Chemical Engineering], E-mail: senthilkumarp@ssn.edu.in

    2010-01-15

    The chrome plating industry is one of the highly polluting industries whose effluent mainly consists of chromium(VI). This compound is highly toxic to aquatic life and human health. The rinse water constituents reflect the chrome plating bath characteristics; generally dead tank wash water contains about 1% of the plating bath concentration. Other metals and metal compounds usually considered as toxic can be precipitated out by suitably adjusting the pH of the wastewaters. However, Cr(VI) is soluble in almost all pH ranges and therefore an efficient treatment is required for the removal and recovery of chromium, and also for the reuse of wastewaters. The present study aims to recover the chromium by a hybrid technique of electrodialysis and ion exchange for the removal and concentration of chromate ions from the effluent. The different modes of operation like batch recirculation process, batch recirculation process with continuous dipping and continuous process were carried out to remove and recover the chromium from the effluent and the percentage reductions of chromium were found to be 98.69%, 99.18% and 100%, respectively. (author)

  13. A hybrid experimental-numerical technique for determining 3D velocity fields from planar 2D PIV data

    Science.gov (United States)

    Eden, A.; Sigurdson, M.; Mezić, I.; Meinhart, C. D.

    2016-09-01

    Knowledge of 3D, three component velocity fields is central to the understanding and development of effective microfluidic devices for lab-on-chip mixing applications. In this paper we present a hybrid experimental-numerical method for the generation of 3D flow information from 2D particle image velocimetry (PIV) experimental data and finite element simulations of an alternating current electrothermal (ACET) micromixer. A numerical least-squares optimization algorithm is applied to a theory-based 3D multiphysics simulation in conjunction with 2D PIV data to generate an improved estimation of the steady state velocity field. This 3D velocity field can be used to assess mixing phenomena more accurately than would be possible through simulation alone. Our technique can also be used to estimate uncertain quantities in experimental situations by fitting the gathered field data to a simulated physical model. The optimization algorithm reduced the root-mean-squared difference between the experimental and simulated velocity fields in the target region by more than a factor of 4, resulting in an average error less than 12% of the average velocity magnitude.

  14. Degradation of Tectilon Yellow 2G by hybrid technique: combination of sonolysis and biodegradation using mutant Pseudomonas putida.

    Science.gov (United States)

    Srinivasan, Raman; Kathiravan, Mathur Nadarajan; Gopinath, Kannappan Panchamoorthy

    2011-02-01

    Degradation of Tectilon Yellow 2G (TY2G), an azo dye has been studied by hybrid technique involving pretreatment by sonochemical method and further biological treatment by Pseudomonas putida mutant. Pretreatment experiments were carried out by sonolysis of the dye solution at different concentrations (100-1000 mg/L). Wild type Gram-negative P. putida species isolated from the textile effluent contaminated soil, which was found to be effective towards dye degradation, has been acclimatized so as to consume TY2G as the sole source of nutrition. Mutant strain was obtained from the acclimatized species by random mutagenesis using the chemical mutagen ethidium bromide for various time intervals (6-30 min). The optimum mutagenesis exposure time for obtaining the most efficient species for dye degradation was found to be 18 min. An efficient mutant strain P. putida ACT 1 has been isolated and was used for growth experiments. The mutant strain showed a better growth compared to the wild strain. The substrate utilization kinetics has been modeled using Monod and Haldane model equations of which the Haldane model provided a better fit. The enzyme kinetics of the mutant and wild species was obtained using Michaelis-Menten equation. The mutated species showed better enzyme kinetics towards the degradation of TY2G.

  15. Hybrid evolutionary techniques in feed forward neural network with distributed error for classification of handwritten Hindi `SWARS'

    Science.gov (United States)

    Kumar, Somesh; Pratap Singh, Manu; Goel, Rajkumar; Lavania, Rajesh

    2013-12-01

    In this work, the performance of feedforward neural network with a descent gradient of distributed error and the genetic algorithm (GA) is evaluated for the recognition of handwritten 'SWARS' of Hindi curve script. The performance index for the feedforward multilayer neural networks is considered here with distributed instantaneous unknown error i.e. different error for different layers. The objective of the GA is to make the search process more efficient to determine the optimal weight vectors from the population. The GA is applied with the distributed error. The fitness function of the GA is considered as the mean of square distributed error that is different for each layer. Hence the convergence is obtained only when the minimum of different errors is determined. It has been analysed that the proposed method of a descent gradient of distributed error with the GA known as hybrid distributed evolutionary technique for the multilayer feed forward neural performs better in terms of accuracy, epochs and the number of optimal solutions for the given training and test pattern sets of the pattern recognition problem.

  16. Heat Treatment Parameters to Optimize Friction and Wear behavior of Novel Hybrid Aluminium Composites Using Taguchi Technique

    Directory of Open Access Journals (Sweden)

    V.C.Uvaraja

    2014-05-01

    Full Text Available In the present study, an Al 7075 alloy is used as the matrix and varying weight percentage of Silicon Carbide (SiC and constant weight percentage of Boron Carbide (B4C as the reinforcing material. The composite is produced using stir casting technique. The composite thus formed is termed as hybrid composite. The samples are prepared for heat treatment process by subjecting to solutionizing temperature of 530o C for 1 hr followed by quenching in water. Further the specimens are subjected to artificial aging for durations of 4, 6 and 8 hr at a temperature of 175°C. The mechanical and tribological properties of composites before and after heat treatment are examined by Vickers hardness test machine and pin-on-disc test machine respectively. The wear rate and friction co-efficient of heat treatment parameters are evaluated based on Taguchi technique. The analysis is further extended to the optimization of test parameters using Design of Experiment (DoE based on L9 orthogonal array. The developed Analysis of Variance (ANOVA and the regression equations is obtained through MINITAB R16 are used to investigate the influence of parameters like sliding speed, applied load, sliding time, and percentage of reinforcement on the dry sliding wear and friction co-efficient of the composites. The wear surface morphology and wear mechanism of the pins are investigated using Scanning Electron Microscope (SEM and are correlated them with wear test results. Finally, confirmation tests are carried out to verify the experimental results.

  17. Development and characterization of interspecific hybrids between Oryza sativa and O. latifolia by in situ hybridization

    Institute of Scientific and Technical Information of China (English)

    YI ChuanDeng; TANG ShuZhu; ZHOU Yong; LIANG GuoHua; GONG ZhiYun; GU MingHong

    2008-01-01

    Oryza sativa and O. latifolia belong to the AA and CCDD genomes of Oryza, respectively. In this study, interspecific hybrids of these species were obtained using the embryo rescue technique. Hybrid pani-cle traits, such as long awns, small grain, exoteric large purple stigma, grain shattering and dispersed panicles, resemble that of the paternal parent, O. latifolia, whereas there is obvious heterosis in such respects as plant height, tillering ability and vegetative vigor. Chromosome pairing and the genomic components of the hybrid were subsequently investigated using genomic in situ hybridization (GISH) and fluorescent in situ hybridization (FISH) analysis. Based on the mitotic metaphase chromosome numbers of the root tips investigated, the hybrid is a triploid with 36 chromosomes. The genomic con-stitution of the hybrid is ACD. In the meiotic metaphase I of the hybrid pollen mother cell, poor chro-mosome pairing was identified and most of the chromosomes were univalent, which resulted in com-plete male sterility in the hybrid.

  18. Application of fluorescence in situ hybridization%荧光原位杂交技术的应用

    Institute of Scientific and Technical Information of China (English)

    陈开慧; 韦赐秋

    2012-01-01

      荧光原位杂交(fluorescence in situ hybridization,FISH)技术是一门新兴的分子细胞遗传学技术,在疾病诊断中发挥了重要作用。本文主要对FISH技术的原理特点以及临床应用作一综述。%  Fluorescence in situ hybridization(FISH) technology is a new promising molecular cytogenetic technique, which plays a vital role in prognosis. This review focuses on the principle and clinical application of FISH.

  19. Fast-FISH Detection and Semi-Automated Image Analysis of Numerical Chromosome Aberrations in Hematological Malignancies

    Directory of Open Access Journals (Sweden)

    Arif Esa

    1998-01-01

    Full Text Available A new fluorescence in situ hybridization (FISH technique called Fast-FISH in combination with semi-automated image analysis was applied to detect numerical aberrations of chromosomes 8 and 12 in interphase nuclei of peripheral blood lymphocytes and bone marrow cells from patients with acute myelogenous leukemia (AML and chronic lymphocytic leukemia (CLL. Commercially available α-satellite DNA probes specific for the centromere regions of chromosome 8 and chromosome 12, respectively, were used. After application of the Fast-FISH protocol, the microscopic images of the fluorescence-labelled cell nuclei were recorded by the true color CCD camera Kappa CF 15 MC and evaluated quantitatively by computer analysis on a PC. These results were compared to results obtained from the same type of specimens using the same analysis system but with a standard FISH protocol. In addition, automated spot counting after both FISH techniques was compared to visual spot counting after standard FISH. A total number of about 3,000 cell nuclei was evaluated. For quantitative brightness parameters, a good correlation between standard FISH labelling and Fast-FISH was found. Automated spot counting after Fast-FISH coincided within a few percent to automated and visual spot counting after standard FISH. The examples shown indicate the reliability and reproducibility of Fast-FISH and its potential for automatized interphase cell diagnostics of numerical chromosome aberrations. Since the Fast-FISH technique requires a hybridization time as low as 1/20 of established standard FISH techniques, omitting most of the time consuming working steps in the protocol, it may contribute considerably to clinical diagnostics. This may especially be interesting in cases where an accurate result is required within a few hours.

  20. Influence of the polymer amount on bioactivity and biocompatibility of SiO{sub 2}/PEG hybrid materials synthesized by sol–gel technique

    Energy Technology Data Exchange (ETDEWEB)

    Catauro, M., E-mail: michelina.catauro@unina2.it [Department of Industrial and Information Engineering, Second University of Naples, Via Roma 29, 81031 Aversa (Italy); Bollino, F.; Papale, F. [Department of Industrial and Information Engineering, Second University of Naples, Via Roma 29, 81031 Aversa (Italy); Gallicchio, M.; Pacifico, S. [Department of Environmental Biological and Pharmaceutical Sciences and Technologies, Second University of Naples, Via Vivaldi 43, 81100 Caserta (Italy)

    2015-03-01

    SiO{sub 2}/PEG organic–inorganic hybrid materials, which differ in polyethylene glycol (PEG) content, were synthesized by sol–gel technique and the characterization of their structure and biological properties was carried out in order to evaluate the possible use in biomedical field. FT-IR spectroscopy detected that the two components of the hybrids (SiO{sub 2} and PEG) are linked by hydrogen bonds between the Si–OH groups of the inorganic phase and the terminal alcoholic groups and/or the ethereal oxygen atoms in the repeating units of polymer. X-ray diffraction analysis ascertained the amorphous nature of the gels and the observation of their morphology by SEM microscopy confirmed that the interpenetration of the two phases (organic and inorganic) occurs on nanometric scale. The biological characterization was carried out as a function of the polymer amount to study its influence on material behavior. The results showed that the synthesized materials were bioactive and biocompatible. The formation of a hydroxyapatite layer, indeed, was observed on their surface by SEM/EDX analysis after soaking in simulated body fluid. Moreover, the biocompatibility of SiO{sub 2}/PEG hybrids was assessed performing MTT and SRB cytotoxicity tests on fibroblast cell NIH 3T3 after 24 and 48 h of exposure, as well as Trypan Blue dye exclusion test. The response to the presence of the investigated materials was positive. The cell growth and proliferation showed dependence on polymer amount and time of exposure to the material extracts. Therefore, the obtained results are encouraging for the use of the obtained hybrids in dental or orthopedic applications. - Highlights: • SiO{sub 2}/PEG hybrid biomaterials synthesized by sol–gel method at various PEG percentages • Chemical and morphological characterization of hybrid materials • Chemical interactions between inorganic and organic components • Biological characterizations with MTT and SRB cytotoxicity tests

  1. Distinguishing between incomplete lineage sorting and genomic introgressions: complete fixation of allospecific mitochondrial DNA in a sexually reproducing fish (Cobitis; Teleostei, despite clonal reproduction of hybrids.

    Directory of Open Access Journals (Sweden)

    Lukas Choleva

    Full Text Available Distinguishing between hybrid introgression and incomplete lineage sorting causing incongruence among gene trees in that they exhibit topological differences requires application of statistical approaches that are based on biologically relevant models. Such study is especially challenging in hybrid systems, where usual vectors mediating interspecific gene transfers--hybrids with Mendelian heredity--are absent or unknown. Here we study a complex of hybridizing species, which are known to produce clonal hybrids, to discover how one of the species, Cobitis tanaitica, has achieved a pattern of mito-nuclear mosaic genome over the whole geographic range. We appplied three distinct methods, including the method using solely the information on gene tree topologies, and found that the contrasting mito-nuclear signal might not have resulted from the retention of ancestral polymorphism. Instead, we found two signs of hybridization events related to C. tanaitica; one concerning nuclear gene flow and the other suggested mitochondrial capture. Interestingly, clonal inheritance (gynogenesis of contemporary hybrids prevents genomic introgressions and non-clonal hybrids are either absent or too rare to be detected among European Cobitis. Our analyses therefore suggest that introgressive hybridizations are rather old episodes, mediated by previously existing hybrids whose inheritance was not entirely clonal. Cobitis complex thus supports the view that the type of resulting hybrids depends on a level of genomic divergence between sexual species.

  2. TM7 detection in human microbiome: are PCR primers and FISH probes specific enough?

    Science.gov (United States)

    Sizova, Maria V.; Doerfert, Sebastian N.; Gavrish, Ekaterina; Epstein, Slava S.

    2015-01-01

    TM7 appears important and omnipresent because it is repeatedly detected by molecular techniques in diverse environments. Here we report that most of primers and FISH probes thought to be TM7-specific do hybridize with multiple species from oral and vaginal cavity. This calls for re-examination of TM7 distribution and abundance. PMID:25957511

  3. Application of fisheries management techniques to assessing impacts: task I report. [Assessment of chemical, radiological, and thermal impacts of nuclear power plants on fish populations

    Energy Technology Data Exchange (ETDEWEB)

    McKenzie, D.H.; Baker, K.S.; Fickeisen, D.H.; Metzger, R.M.; Skalski, J.R.

    1979-03-01

    Task I efforts examined the available fisheries management techniques and assessed their potential application in a confirmatory monitoring program. The objective of such monitoring programs is to confirm that the prediction of an insignificant impact (usually made in the FES) was correct. Fisheries resource managers have developed several tools for assessing the fish population response to stress (exploitation) and they were thought potentially useful for detecting nuclear power plant impacts. Techniques in three categories were examined; catch removal, population dynamics, and nondestructive censuses, and the report contains their description, examples of application, advantages, and disadvantages. The techniques applied at nuclear power plant sites were examined in detail to provide information on implementation and variability of specific approaches. The most suitable techniques to incorporate into a monitoring program confirming no impact appear to be those based on Catch Per Unity Effort (CPUE) and hydroacoustic data. In some specific cases, age and growth studies and indirect census techniques may be beneficial. Recommendations for task II efforts to incorporate these techniques into monitoring program designs are presented. These include development of guidelines for; (1) designing and implementing a data collection program; (2) interpreting these data and assessing the occurrence of impact, and (3) establishment of the monitoring program's ability to detect changes in the affected populations.

  4. Hybrid-SAR Technique: Joint Analysis Using Phase-Based and Amplitude-Based Methods for the Xishancun Giant Landslide Monitoring

    Directory of Open Access Journals (Sweden)

    Tengteng Qu

    2016-10-01

    Full Text Available Early detection and early warning are of great importance in giant landslide monitoring because of the unexpectedness and concealed nature of large-scale landslides. In China, the western mountainous areas are prone to landslides and feature many giant complex landslides, especially following the Wenchuan Earthquake in 2008. This work concentrates on a new technique, known as the “hybrid-SAR technique”, that combines both phase-based and amplitude-based methods to detect and monitor large-scale landslides in Li County, Sichuan Province, southwestern China. This work aims to develop a robust methodological approach to promptly identify diverse landslides with different deformation magnitudes, sliding modes and slope geometries, even when the available satellite data are limited. The phase-based and amplitude-based techniques are used to obtain the landslide displacements from six TerraSAR-X Stripmap descending scenes acquired from November 2014 to March 2015. Furthermore, the application circumstances and influence factors of hybrid-SAR are evaluated according to four aspects: (1 quality of terrain visibility to the radar sensor; (2 landslide deformation magnitude and different sliding mode; (3 impact of dense vegetation cover; and (4 sliding direction sensitivity. The results achieved from hybrid-SAR are consistent with in situ measurements. This new hybrid-SAR technique for complex giant landslide research successfully identified representative movement areas, e.g., an extremely slow earthflow and a creeping region with a displacement rate of 1 cm per month and a typical rotational slide with a displacement rate of 2–3 cm per month downwards and towards the riverbank. Hybrid-SAR allows for a comprehensive and preliminary identification of areas with significant movement and provides reliable data support for the forecasting and monitoring of landslides.

  5. X射线检测海水鱼片中鱼刺%Application of X-Ray Technique to Detection of Fish Bones in Marine Fish Fillets

    Institute of Scientific and Technical Information of China (English)

    胡记东; 刘远平; 孙爱华; 林洪; 郭晓华; 年睿; 李钰金; 曹立民

    2016-01-01

    This study aimed to examine the effectiveness of X-ray technique in the detection of fish bones with respect to three aspects: optimization of working parameters of X-ray detector, analysis of fish bone distribution pattern and combined application of X-ray technique and freezing technique. The results showed that for most marine fish fillets, the optimal detection parameters were determined as fol ows: voltage range, 30–45 kV; current range, 3 000–6 000 μA; and brightness range, 4–6. In term of fish bone distribution, the detection rates of fish bones in frozen fish fillets were higher than those in unfrozen state, and artificial judgment of X-ray images for fish bones is more practical. By optimizing X-ray detector parameters, ascertaining fish bone distribution pattern and combining freezing technique and X-ray technique, the quality of X-ray image, the detection rate of fish bones and the visual sense of X-ray test personnel in aquatic products processing enterprises were improved. Therefore, the problem of remaining fish bones in frozen marine fish fillets can be solved to a certain extent.%通过X光机设备参数优化、鱼刺分布规律总结及冷冻结合X光机技术3个方面对X射线检测鱼刺效果进行研究。结果表明,大部分海产鱼片产品的最优检测参数为:电压范围30~45 kV、电流范围3000~6000μA、亮度范围4~6;通过鱼刺分布规律总结,鱼片在冷冻状态下鱼刺的检出率均高于解冻状态下鱼刺检出率,且冷冻状态下人工读X射线图判断鱼刺更接近于实际情况。通过X光机设备参数优化、鱼刺分布规律总结的

  6. Assessment of fusion facility dose rate map using mesh adaptivity enhancements of hybrid Monte Carlo/deterministic techniques

    Energy Technology Data Exchange (ETDEWEB)

    Ibrahim, Ahmad M., E-mail: ibrahimam@ornl.gov [Oak Ridge National Laboratory, P.O. Box 2008, Oak Ridge, TN 37831 (United States); Wilson, Paul P. [University of Wisconsin-Madison, 1500 Engineering Dr., Madison, WI 53706 (United States); Sawan, Mohamed E., E-mail: sawan@engr.wisc.edu [University of Wisconsin-Madison, 1500 Engineering Dr., Madison, WI 53706 (United States); Mosher, Scott W.; Peplow, Douglas E.; Grove, Robert E. [Oak Ridge National Laboratory, P.O. Box 2008, Oak Ridge, TN 37831 (United States)

    2014-10-15

    Highlights: •Calculate the prompt dose rate everywhere throughout the entire fusion energy facility. •Utilize FW-CADIS to accurately perform difficult neutronics calculations for fusion energy systems. •Develop three mesh adaptivity algorithms to enhance FW-CADIS efficiency in fusion-neutronics calculations. -- Abstract: Three mesh adaptivity algorithms were developed to facilitate and expedite the use of the CADIS and FW-CADIS hybrid Monte Carlo/deterministic techniques in accurate full-scale neutronics simulations of fusion energy systems with immense sizes and complicated geometries. First, a macromaterial approach enhances the fidelity of the deterministic models without changing the mesh. Second, a deterministic mesh refinement algorithm generates meshes that capture as much geometric detail as possible without exceeding a specified maximum number of mesh elements. Finally, a weight window coarsening algorithm decouples the weight window mesh and energy bins from the mesh and energy group structure of the deterministic calculations in order to remove the memory constraint of the weight window map from the deterministic mesh resolution. The three algorithms were used to enhance an FW-CADIS calculation of the prompt dose rate throughout the ITER experimental facility and resulted in a 23.3% increase in the number of mesh tally elements in which the dose rates were calculated in a 10-day Monte Carlo calculation. Additionally, because of the significant increase in the efficiency of FW-CADIS simulations, the three algorithms enabled this difficult calculation to be accurately solved on a regular computer cluster, eliminating the need for a world-class super computer.

  7. Leptin stimulates hepatic growth hormone receptor and insulin-like growth factor gene expression in a teleost fish, the hybrid striped bass.

    Science.gov (United States)

    Won, Eugene T; Douros, Jonathan D; Hurt, David A; Borski, Russell J

    2016-04-01

    Leptin is an anorexigenic peptide hormone that circulates as an indicator of adiposity in mammals, and functions to maintain energy homeostasis by balancing feeding and energy expenditure. In fish, leptin tends to be predominantly expressed in the liver, another important energy storing tissue, rather than in fat depots as it is in mammals. The liver also produces the majority of circulating insulin-like growth factors (IGFs), which comprise the mitogenic component of the growth hormone (GH)-IGF endocrine growth axis. Based on similar regulatory patterns of leptin and IGFs that we have documented in previous studies on hybrid striped bass (HSB: Morone saxatilis×Morone chrysops), and considering the co-localization of these peptides in the liver, we hypothesized that leptin might regulate the endocrine growth axis in a manner that helps coordinate somatic growth with energy availability. Using a HSB hepatocyte culture system to simulate autocrine or paracrine exposure that might occur within the liver, this study examines the potential for leptin to modulate metabolism and growth through regulation of IGF gene expression directly, or indirectly through the regulation of GH receptors (GHR), which mediate GH-induced IGF expression. First, we verified that GH (50nM) has a classical stimulatory effect on IGF-1 and additionally show it stimulates IGF-2 transcription in hepatocytes. Leptin (5 and/or 50nM) directly stimulated in vitro GHR2 gene expression within 8h of exposure, and both GHR1 and GHR2 as well as IGF-1 and IGF-2 gene expression after 24h. Cells were then co-incubated with submaximal concentrations of leptin and GH (25nM each) to test if they had a synergistic effect on IGF gene expression, possibly through increased GH sensitivity following GHR upregulation by leptin. In combination, however, the treatments only had an additive effect on stimulating IGF-1 mRNA despite their capacity to increase GHR mRNA abundance. This suggests that leptin's stimulatory

  8. Picoplankton Community Composition by CARD-FISH and Flow Cytometric Techniques: A Preliminary Study in Central Adriatic Sea Water

    Directory of Open Access Journals (Sweden)

    Anita Manti

    2012-01-01

    Full Text Available Data concerning picoplanktonic community composition and abundance in the Central Adriatic Sea are presented in an effort to improve the knowledge of bacterioplankton and autotrophic picoplankton and their seasonal changes. Flow cytometry analyses revealed the presence of two distinct bacteria populations: HNA and LNA cells. HNA cells showed an explicit correlation with viable and actively respiring cells. The study of viability and activity may increase our knowledge of the part that contributes really to the remineralization and bacterial biomass production. Authotrophic picoplankton abundance, especially picocyanobacteria, was strongly influenced by seasonality, indicating that light availability and water temperature are very important regulating factors. In terms of total carbon biomass, the main contribution came from heterotrophic bacteria with a lower contribution from autotrophic picoplankton. CARD-FISH evidenced, within the Eubacteria domain, the dominance of members of the phyla Alphaproteobacteria, with a strong contribution from SAR11clade, followed by Cytophaga-Flavobacterium and Gammaproteobacteria. The bacterial groups detected contributed differently depending when the sample was taken, suggesting possible seasonal patterns. This study documents for the first time picoplankton community composition in the Central Adriatic Sea using two different approaches, FCM and CARD-FISH, and could provide preliminary data for future studies.

  9. Analysis, control and optimal operations in hybrid power systems advanced techniques and applications for linear and nonlinear systems

    CERN Document Server

    Bizon, Nicu; Mahdavi Tabatabaei, Naser

    2014-01-01

    This book explains and analyzes the dynamic performance of linear and nonlinear systems, particularly for Power Systems including Hybrid Power Sources. Offers a detailed description of system stability using state space energy conservation principle, and more.

  10. TiO{sub 2}/PCL hybrid materials synthesized via sol–gel technique for biomedical applications

    Energy Technology Data Exchange (ETDEWEB)

    Catauro, M., E-mail: michelina.catauro@unina2.it [Department of Industrial and Information Engineering, Second University of Naples, Via Roma 29, 81031 Aversa (Italy); Bollino, F.; Papale, F. [Department of Industrial and Information Engineering, Second University of Naples, Via Roma 29, 81031 Aversa (Italy); Marciano, S.; Pacifico, S. [Department Environmental, Biological and Pharmaceutical Sciences and Technologies, Second University of Naples, Via Vivaldi 43, 81100 Caserta (Italy)

    2015-02-01

    The aim of the present work has been the synthesis of organic/inorganic hybrid materials based on titanium dioxide and poly(ε-caprolactone) (PCL) to be used in the biomedical field. Several materials have been synthesized using sol–gel methods by adding different amounts of polymer to the inorganic sol. The obtained gels have been characterized using Fourier transform infrared (FT-IR) spectroscopy, scanning electron microscopy (SEM) and atomic force microscopy (AFM). The FT-IR data allowed us to hypothesize that the structure formed was that of an interpenetrating network, realized by hydrogen bonds between Ti-OH groups in the sol–gel intermediate species and carbonyl groups in the polymer repeating units. SEM and AFM analyses highlighted that the obtained materials were nanostructurated hybrids. To evaluate the biological properties of the hybrids, their bioactivity and cytotoxicity were investigated as a function of the PCL amount. The bioactivity of the synthesized systems was proven by the formation of a hydroxyapatite layer on the surface of samples soaked in a fluid simulating human blood plasma (SBF). MTT cytotoxicity tests and Trypan Blue dye exclusion tests were carried out exposing NIH-3T3 mouse embryonic fibroblasts for 24 and 48 h to extracts from the investigated hybrid materials. The results showed that all the hybrids had a non-cytotoxic effect on target cells. - Highlights: • TiO{sub 2}/PCL hybrids were obtained by the sol–gel process for biomedical applications. • Synthesized materials were found to be first-class hybrid nanocomposites. • Hybrids appear to be bioactive, a fundamental characteristic for osseointegration. • MTT and Trypan Blue viability test show that the materials are biocompatible. • The organic phase is able to modulate the biocompatibility of the materials.

  11. Investigation of kinetics and thermodynamics of DNA hybridization by means of 2-D fluorescence spectroscopy and soft/hard modeling techniques

    Energy Technology Data Exchange (ETDEWEB)

    Ebrahimi, Sara; Kompany-Zareh, Mohsen, E-mail: kmpz@dr.com

    2016-02-04

    Reversible hybridization reaction plays a key role in fundamental biological processes, in many laboratory techniques, and also in DNA based sensing devices. Comprehensive investigation of this process is, therefore, essential for the development of more sophisticated applications. Kinetics and thermodynamics of the hybridization reaction, as a second order process, are systematically investigated with the aid of the soft and hard chemometric methods. Labeling two complementary 21 mer DNA single strands with FAM and Texas red fluorophores, enabled recording of the florescence excitation−emission matrices during the experiments which led to three-way data sets. The presence of fluorescence resonance energy transfer in excitation and emission modes and the closure in concentration mode, made the three-way data arrays rank deficient. To acquire primary chemical information, restricted Tucker3 as a soft method was employed. Herein a model-based method, hard restricted trilinear decomposition, is introduced for in depth analysis of rank deficient three-way data sets. By employing proposed hard method, the nonlinear model parameters as well as the correct profiles could be estimated. In addition, a simple constraint is presented to extract chemically reasonable output profiles regarding the core elements of restricted Tucker3 model. - Highlights: • Hard restricted trilinear decomposition (HrTD) was introduced for model-based analysis of three-way rank deficient data. • DNA hybridization was investigated by two-dimensional fluorescence spectroscopy and soft/hard multi-way techniques. • Restricted Tucker3 analysis enabled accurate estimation of pure FRET profiles in the hybridized form. • HrTD was successfully employed to estimate kinetic and equilibrium parameters of DNA hybridization system. • The performance of the proposed methods in response to different physical stimuli was successfully evaluated.

  12. Identification of Cannabis sativa L. using the 1-kbTHCA synthase-fluorescence in situ hybridization probe.

    Science.gov (United States)

    Jeangkhwoa, Pattraporn; Bandhaya, Achirapa; Umpunjun, Puangpaka; Chuenboonngarm, Ngarmnij; Panvisavas, Nathinee

    2017-03-01

    This study reports a successful application of fluorescence in situ hybridization (FISH) technique in the identification of Cannabis sativa L. cells recovered from fresh and dried powdered plant materials. Two biotin-16-dUTP-labeled FISH probes were designed from the Cannabis-specific tetrahydrocannabinolic acid synthase (THCAS) gene and the ITS region of the 45S rRNA gene. Specificity of probe-target hybridization was tested against the target and 4 non-target plant species, i.e., Humulus lupulus, Mitragyna speciosa, Papaver sp., and Nicotiana tabacum. The 1-kb THCA synthase hybridization probe gave Cannabis-specific hybridization signals, unlike the 700-bp Cannabis-ITS hybridization probe. Probe-target hybridization was also confirmed against 20 individual Cannabis plant samples. The 1-kb THCA synthase and 700-bp Cannabis-ITS hybridization probes clearly showed 2 hybridization signals per cell with reproducibility. The 1-kb THCA synthase probe did not give any FISH signal when tested against H. lupulus, its closely related member of the Canabaceae family. It was also showed that 1-kb THCA synthase FISH probe can be applied to identify small amount of dried powdered Cannabis material with an addition of rehydration step prior to the experimental process. This study provided an alternative identification method for Cannabis trace. Copyright © 2016. Published by Elsevier B.V.

  13. BIOTECHNOLOGY OF THE FISH AQUACULTURE

    Directory of Open Access Journals (Sweden)

    L. P. Buchatsky

    2013-12-01

    Full Text Available The latest progress in biotechnology on fish aquaculture and different modern methods of investigations for increasing of fish productivity in aquaculture are analyzed. Except for the applied aspect, the use of modern biotechnological methods of investigations opens new possibilities for fundamental researches of sex-determining mechanisms, polyploidy, distant hybridization, and developmental biology of bony fishes. Review contains examples of utilizing modern biotechnology methods to obtain transgenic fishes with accelerated growth and for designing surrogate fishes. Methods for receiving unisexual shoals of salmon and sturgeon female fishes with the view of obtaining a large quantity of caviar, as well as receiving sterile (triploid fishes are analyzed. Great attention is given to androgenesis, particularly to disperm one, in connection with the problem of conserving rare and vanishing fish species using only sperm genetic material. Examples how distant hybrids may be obtained with the use of disperm androgenesis and alkylated DNA are given. Methods of obtaining fish primordium germ cells, recent developments in cultivation of fish stem cells and their use in biotechnology, as well as ones of transplantation of oogonium and spermatogonium to obtain surrogate fishes. The examples of successful experiments on spermatogonial xenotransplantation and characteristic of antifreezing fish proteins and also the prospect of their practical usage are given.

  14. Fish Allergy

    Science.gov (United States)

    ... Old Feeding Your 1- to 2-Year-Old Fish Allergy KidsHealth > For Parents > Fish Allergy Print A ... From Home en español Alergia al pescado About Fish Allergy A fish allergy is not exactly the ...

  15. 饲草高梁杂交种吉草3号的选育及栽培技术%Breeding and Cultivation Technique of Forage Sorghum Hybrid of Jicao 3

    Institute of Scientific and Technical Information of China (English)

    李继洪; 李淑杰; 胡喜连; 陈冰嬬; 高士杰

    2012-01-01

    Jicao 3 is one kind of forage sorghum hybrid crossed by Institute of Crop Breeding, Jilin Academy of Agricultural Sciences in 2004. Jicao 3 was examined and approved by National Sorghum Varieties Identification Committee in February, 2012. The hybrid has some features of fast growth rate, strong regeneration ability and adaptability, high biological yield and nutritional value. It was not only fine quality forage of bovine, goat, fish, rabbit and goose and so on, but also elite grass seed to improve ecological environment. In order to promote the development of animal husbandry in China and increase the feed source for the development of herbivorous animals, the breeding and cultivation techniques of Jicao 3 were studied and concluded.%吉草3号是吉林省农业科学院作物育种研究所于2004年组配而成的饲草高粱新品种。2012年2月通过国家高粱品种鉴定委员会鉴定。该杂交种具有生长速度快、再生能力强、生物产量高、营养价值高、适应性强、适口性好等特点,是牛、羊、鱼、兔、鹅等动物的优质饲草,也是改善生态环境的优良草种。为了促进我国畜牧业的发展,并为我国草食畜养殖业的发展增加饲料来源,对饲草高梁新品种吉草3号的选育及栽培技术进行了研究与总结。

  16. Modified cIg-FISH protocol for multiple myeloma in routine cytogenetic laboratory practice.

    Science.gov (United States)

    Gole, Leena; Lin, Adeline; Chua, Constance; Chng, Wee Joo

    2014-01-01

    The International Myeloma Working Group recommends that fluorescence in situ hybridization (FISH) be performed on specifically identified plasma cells (PC). This is because chromosomal abnormalities are not frequently detected by traditional karyotyping due to the low proliferative rate of PC in multiple myeloma (MM). Conventional FISH enhances the sensitivity but lacks the specificity, as it does not distinguish PC from other hematopoetic cells. To fulfill this recommendation, PC need to be selected either by flow cytometry or immunomagnetic bead-based PC sorting or by concomitant labeling of the cytoplasmic immunoglobulin light chain, which allows for unambiguous identification. These techniques require expertise, time, and funding and are not easily incorporated into the routine workflow of the cytogenetic laboratory. We have modified and refined the technique using fixed cell pellets to achieve nicely separated and easily identifiable PC. With immunostaining and subsequent FISH (i.e., cytoplasmic immunoglobulin FISH, cIg-FISH), this technique can be easily incorporated into every cytogenetic laboratory. Twenty samples from patients with MM were subjected to routine FISH, cIg-FISH, and chromosomal karyotyping and the results were compared. Three FISH probes, which enabled detection of the t(4;14), t(14;16) and deletion of TP53, were used to validate this modified technique successfully. Copyright © 2014 Elsevier Inc. All rights reserved.

  17. Coupling physical chemical techniques with hydrotalcite-like compounds to exploit their structural features and new multifunctional hybrids with luminescent properties.

    Science.gov (United States)

    Costantino, Umberto; Costantino, Ferdinando; Elisei, Fausto; Latterini, Loredana; Nocchetti, Morena

    2013-08-28

    Hydrotalcite-like compounds (HTlc), belonging to the large class of Layered Double Hydroxides (LDH), have excited wide interest owing to the incredible number of their potential and achieved applications in physical, chemical and bio-chemical fields. This perspective review deals with recent advances in the application of physical-chemical techniques for the study of HTlc structure and for the design and synthesis, using intercalation chemistry routes, of new hybrid materials. Firstly, a rapid survey on the most common synthetic strategies for the attainment of HTlc with different crystallinity degree and crystal size and for their modification to obtain hybrids has been made, and the use of coupled techniques (XRPD, luminescence, Solid State MAS NMR and Molecular Dynamics) to gain structural information is reported. Then, the design, synthesis and photophysical characterization of azoic dyes-intercalated and co-intercalated HTlc hybrid materials are described. Hybrids constituted of ZnAl-HTlc, co-intercalated with stearate anions and methyl orange or methyl yellow dyes, have been used as nanofillers of hydrophobic polymers. The polymeric nano-composites obtained have been characterized by means of XRPD patterns, Thermo-Gravimetric Analysis and Confocal Fluorescence Microscopy. This latter technique has been found to be an excellent, complementary and non-invasive tool to probe the dispersion degree of the fluorescent fillers into the polymeric matrices and their stability in the compounding process. Finally, the synthesis and spectroscopic characterization of nanoparticle (NP) decorated HTlc for advanced antimicrobial and photo-catalytic applications are also reported. The review terminates with a concluding short note and future trends.

  18. Cervical anterior hybrid technique with bi-level Bryan artificial disc replacement and adjacent segment fusion for cervical myelopathy over three consecutive segments.

    Science.gov (United States)

    Chen, Jiang; Xu, Lin; Jia, Yu-Song; Sun, Qi; Li, Jin-Yu; Zheng, Chen-Ying; Bai, Chun-Xiao; Yu, Qin-Sheng

    2016-05-01

    This study aimed to assess the preliminary clinical efficacy and feasibility of the hybrid technique for multilevel cervical myelopathy. Considering the many shortcomings of traditional treatment methods for multilevel cervical degenerative myelopathy, hybrid surgery (bi-level Bryan artificial disc [Medtronic Sofamor Danek, Memphis, TN, USA] replacement and anterior cervical discectomy and fusion) should be considered. Between March 2006 and November 2012, 108 patients (68 men and 40 women, average age 45years) underwent hybrid surgery. Based on the Japanese Orthopaedic Association (JOA) score, Neck Disability Index (NDI), and Odom's criteria, the clinical symptoms and neurological function before and after surgery were evaluated. Mean surgery duration was 90minutes, with average blood loss of 30mL. Mean follow-up duration was 36months. At the final follow-up, the mean JOA (± standard deviation) scores were significantly higher compared with preoperative values (15.08±1.47 versus 9.18±1.22; P<0.01); meanwhile, NDI values were markedly decreased (12.32±1.03 versus 42.68±1.83; P<0.01). Using Odom's criteria, the clinical outcomes were rated as excellent (76 patients), good (22 patients), fair (six patients), and poor (four patients). These findings indicate that the hybrid method provides an effective treatment for cervical myelopathy over three consecutive segments, ensuring a good clinical outcome. Copyright © 2015 Elsevier Ltd. All rights reserved.

  19. A Novel Hybrid Ultramicrotomy/FIB-SEM Technique: Preparation of Serial Electron-Transparent Thin Sections of a Hayabusa Grain

    Science.gov (United States)

    Berger, Eve L.; Keller, Lindsay P.

    2014-01-01

    The Japanese space agency's (JAXA) Hayabusa mission returned the first particulate samples (typically SEM). Using a diamond trim knife, the epoxy surrounding the grain is removed on 3 sides (to within a few microns of the grain); the depth of material removed extends well below the bottom of the particle. The sample is attached to an SEM pin mount, the epoxy coated with conductive paint, and the entire assembly coated with approx. 40nm of carbon to eliminate sample charging during FIB work. A protective carbon cap is placed according to the plan for the 15 FIB sections. The central 'spine' of the cap runs perpendicular to the front of the sample, and the 'ribs' protruding from either side run parallel. Each rib indicates the location of a planned FIB section, and the spine contains the final two planned sections. We use a cap with a 4 micron-wide spine and 2micron-wide ribs that have ?3.5 micron of space between them (narrower cuts result in too much re-deposition of material inside the trenches). Using a 30kV, 3nA ion-beam we expose the front surface of the grain and commence milling trenches between sections. Rather than using the typical C-cut to prepare the sample for lift-out, an L-cut is used instead, leaving the sample connected by an interior tab. tab. Sections are lifted out, attached to TEM grids and thinned to electron transparency. TEM analyses show that our hybrid technique preserves both interior and edge features, including surface modifications from exposure to the space environment, such as damaged rims that form in response to solar wind implantation effects and adhering grains. In addition, the FIB sections provide larger areas that are free of fractures and chatter effects in comparison to the microtome thin sections, thus enabling more accurate measurements of solar flare particle track densities that are used to determine the surface exposure age of the particles.

  20. Insights into food preference in hybrid F1 of Siniperca chuatsi (♀) × Siniperca scherzeri (♂) mandarin fish through transcriptome analysis.

    Science.gov (United States)

    He, Shan; Liang, Xu-Fang; Sun, Jian; Li, Ling; Yu, Ying; Huang, Wei; Qu, Chun-Mei; Cao, Liang; Bai, Xiao-Li; Tao, Ya-Xiong

    2013-09-05

    As economically relevant traits, feeding behavior and food preference domestication determine production cost and profitability. Although there are intensive research efforts on feeding behavior and food intake, little is known about food preference. Mandarin fish accept only live prey fish and refuse dead prey fish or artificial diets. Very little is currently known about the genes regulating this unique food preference. Using transcriptome sequencing and digital gene expression profiling, we identified 1,986 and 4,526 differentially expressed genes in feeders and nonfeeders of dead prey fish, respectively. Up-regulation of Crbp, Rgr and Rdh8, and down-regulation of Gc expression, consistent with greater visual ability in feeders, could promote positive phototaxis. Altered expressions of period, casein kinase and Rev-erbα might reset circadian phase. Down-regulation of orexigenic and up-regulation of anorexigenic genes in feeders were associated with lower appetite. The mRNA levels of Creb, c-fos, C/EBP, zif268, Bdnf and Syt were dramatically decreased in feeders, which might result in significant deficiency in memory retention of its natural food preference (live prey fish). There were roughly 100 times more potential SNPs in feeders than in nonfeeders. In summary, differential expression in the genes identified shed new light on why mandarin fish only feed on live prey fish, with pathways regulating retinal photosensitivity, circadian rhythm, appetite control, learning and memory involved. We also found dramatic difference in SNP abundance in feeders vs nonfeeders. These differences together might account for the different food preferences. Elucidating the genes regulating the unique food preference (live prey fish) in mandarin fish could lead to a better understanding of mechanisms controlling food preference in animals, including mammals.

  1. 荧光原位杂交技术在膀胱癌诊断中的应用%Diagnosis of Bladder Cancer Using Fluorescence in Situ Hybridization Technique

    Institute of Scientific and Technical Information of China (English)

    陈文轩; 汤坤龙; 郭洪波; 彭杰; 林毅

    2011-01-01

    Objective To evaluate the value of using fluorescence in situ hybridization (FISH)technique for the detection of chromosome aberration of urine exfoliated cells for the diagnosis of bladder tumor.Methods FISH technique were used to detect the abnormalities of chromosome 3,7,17 and 9p16 site from 20 normal people, and to establish the threshold.The morning's first urinations were available from 75 patients with bladder cancer and 25 patients without urothelial tumor, then were detected using FISH technique and urine cytology respectively.The sample was considered positive if two or more probes results higher than the criteria,or one probe has two or more abnormal results.Results The sensitivity of single using were 73.3% (55/75),76.0% (57/75),62.7% (47/75) and 62.7% (47/75) for the 4 probes (3,7, 17 and 9p16)respectively.The sensitivity of combined detection was 85.3% (64/75) and specificity was 96.0% (24/25) The sensitivity and specificity of urine cytology examination was 9.3% (7/75) and 100% (25/25) .The sensitivity of FISH examination was significantly higher than that of urine cytology examination (85.3% vs 9.3% ,x2 = 57.00, P < 0.001) .Sensitivity of FISH examination was not correlated with cancer pathologic grading(low vs high : 84.2% vs 86.5%, x2 = 0.08, P > 0.05) and clinical stage (ta-tl : 82.9%, t2-t4 :87.5%, x2 = 0.32 ,P > 0.05) .Conclusion FISH technique is a non-invasive and effective method for the early diagnosis of bladder tumor and is more sensitive than urine cytology.Furthermore, FISH technique can be used to predict the tumor's biological behavivor and prognosis.%目的 探讨荧光原位杂交(FISH)检测尿脱落细胞染色体畸变在膀胱癌诊断中的临床应用价值。方法 采用3、7及17号染色体着丝粒特异性探针及9号染色体p16位点特异性探针对20名健康人(正常组)新鲜晨尿进行FISH检测,建立阈值。留取75例膀胱癌(膀胱癌组)患者和25例非膀胱癌

  2. Progress in transgenic fish techniques and application%转基因鱼的研究进展与商业化前景

    Institute of Scientific and Technical Information of China (English)

    叶星; 田园园; 高风英

    2011-01-01

    转基因技术为鱼类育种开辟了新的途径.目前已培育出转生长激素基因鲤,鲑和罗非鱼,转荧光蛋白基因斑马鱼与唐鱼等可稳定遗传的转基因鱼品系,其中快长转生长激素基因鱼的获得对于提高水产养殖的产量与养殖效益具有十分重要的意义.文章简要综述了转基因鱼应用研究的成就、相关技术及生态安全方面的研究进展.显微注射仍是目前基因转植的常用方法,应用转座酶或巨核酸酶介导的转基因新技术可提高基因转植效率与整合率.转基因元件的选择应尽量考虑"全鱼"基因或"自源"基因,以减少转基因鱼食用安全方面的顾虑同时也有利于转植基因的表达与生理功效的发挥.生态安全是转基因鱼商用化面临的最大问题.虽然有研究显示转基因鱼与传统的选育鱼类相比适合度较差,但由于环境与基因型间的相互作用,根据实验室获得的转基因鱼对生态影响的结果,难以预测转基因鱼一旦逃逸会对自然水生态环境产生怎样的影响.因此应建立高度自然化的环境以获得可靠的数据客观评价生态风险,有效的物理拦截、不育化处理等生物学控制策略仍是保证转基因鱼安全应用的关键措施.%Transgenic technique provides a new way for fish breeding.Stable lines of growth hormone gene transfer carps,salmon and tilapia, as well as fluorescence protein gene transfer zebra fish and white cloud mountain minnow have been produced.The fast growth characteristic of GH gene transgenic fish will be of great importance to promote aquaculture production and economic efficiency.This paper summarized the progress in transgenic fish research and ecological assessments.Microinjection is still the most common used method, but often resulted in multi-site and multi-copies integration.Co-injection of transposon or meganuclease will greatly improve the efficiency of gene transfer and integration."All fish"gene or

  3. BIOTECHNOLOGY OF THE FISH AQUACULTURE

    OpenAIRE

    L. P. Buchatsky

    2013-01-01

    The latest progress in biotechnology on fish aquaculture and different modern methods of investigations for increasing of fish productivity in aquaculture are analyzed. Except for the applied aspect, the use of modern biotechnological methods of investigations opens new possibilities for fundamental researches of sex-determining mechanisms, polyploidy, distant hybridization, and developmental biology of bony fishes. Review contains examples of utilizing modern biotechnology methods to obtain ...

  4. Measuring fish and their physical habitats: Versatile 2D and 3D video techniques with user-friendly software

    Science.gov (United States)

    Neuswanger, Jason R.; Wipfli, Mark S.; Rosenberger, Amanda E.; Hughes, Nicholas F.

    2017-01-01

    Applications of video in fisheries research range from simple biodiversity surveys to three-dimensional (3D) measurement of complex swimming, schooling, feeding, and territorial behaviors. However, researchers lack a transparently developed, easy-to-use, general purpose tool for 3D video measurement and event logging. Thus, we developed a new measurement system, with freely available, user-friendly software, easily obtained hardware, and flexible underlying mathematical methods capable of high precision and accuracy. The software, VidSync, allows users to efficiently record, organize, and navigate complex 2D or 3D measurements of fish and their physical habitats. Laboratory tests showed submillimetre accuracy in length measurements of 50.8 mm targets at close range, with increasing errors (mostly <1%) at longer range and for longer targets. A field test on juvenile Chinook salmon (Oncorhynchus tshawytscha) feeding behavior in Alaska streams found that individuals within aggregations avoided the immediate proximity of their competitors, out to a distance of 1.0 to 2.9 body lengths. This system makes 3D video measurement a practical tool for laboratory and field studies of aquatic or terrestrial animal behavior and ecology.

  5. Epidermal Growth Factor Receptor (EGFR gene copy number (GCN correlates with clinical activity of irinotecan-cetuximab in K-RAS wild-type colorectal cancer: a fluorescence in situ (FISH and chromogenic in situ hybridization (CISH analysis

    Directory of Open Access Journals (Sweden)

    Scartozzi Mario

    2009-08-01

    Full Text Available Abstract Background K-RAS wild type colorectal tumors show an improved response rate to anti-EGFR monoclonal antibodies. Nevertheless 70% to 40% of these patients still does not seem to benefit from this therapeutic approach. FISH EGFR GCN has been previously demonstrated to correlate with clinical outcome of colorectal cancer treated with anti-EGFR monoclonal antibodies. CISH also seemed able to provide accurate EGFR GCN information with the advantage of a simpler and reproducible technique involving immunohistochemistry and light microscopy. Based on these findings we investigated the correlation between both FISH and CISH EGFR GCN and clinical outcome in K-RAS wild-type colorectal cancer treated with irinotecan-cetuximab. Methods Patients with advanced K-RAS wild-type, colorectal cancer receiving irinotecan-cetuximab after failure of irinotecan-based chemotherapy were eligible. A cut-off value for EGFR GCN of 2.6 and 2.12 for FISH and CISH respectively was derived from ROC curve analysis. Results Forty-four patients were available for analysis. We observed a partial remission in 9 (60% and 2 (9% cases with a FISH EGFR GCN ≥ 2.6 and Conclusion FISH and CISH EGFR GCN may both represent effective tools for a further patients selection in K-RAS wild-type colorectal cancer treated with cetuximab.

  6. Fusobacterium necrophorum determined as abortifacient in sheep by laser capture microdissection and fluorescence in situ hybridization

    DEFF Research Database (Denmark)

    Boye, Mette; Aalbæk, Bent; Agerholm, Jørgen S.

    2006-01-01

    at late pregnancy by a technique that combines laser capture microdissection (LCM) and fluorescent in situ hybridization (LCM-FISH). Cultural bacteriological examination had failed to identify an infectious agent but by histological examination, large colonies of bacteria associated with tissue......Fluorescent in situ hybridization (FISH) has been extensively used for identification of individual microbial cells within their natural environment. The present work describes the identification of Fusobacterium necrophorum in formalin-fixed tissue samples from three sets of ovine twins aborted......RNA-targeting oligonucleotide probe specific for F. necrophorum was used in a FISH assay. In situ hybridization showed a high density of F. necrophorum in all examined tissue sections. Simultaneous probing with a general bacterial probe EUB338 and the specific probe for F. necrophorum showed that no other bacteria could...

  7. Clinicopathological and molecular spectrum of ewing sarcomas/PNETs, including validation of EWSR1 rearrangement by conventional and array FISH technique in certain cases.

    Science.gov (United States)

    Rekhi, Bharat; Vogel, Ulrich; Basak, Ranjan; Desai, Sangeeta B; Jambhekar, Nirmala A

    2014-07-01

    Over the years, a wide clinicopathological spectrum has been identified within Ewing family of tumors (EFTs). As these tumors are chemosensitive, their correct and timely identification is necessary. The aims of this study were (1) to present the diverse clinicopathological and molecular profile of EFTs in our settings, (2) to identify a pragmatic approach for diagnosing EFTs, especially for application of ancillary techniques, namely RT-PCR for specific transcripts (EWS-FLI1, EWS-ERG) and FISH for EWSR1 gene rearrangement, in certain cases and (3) to show the utility of tissue microarray in establishing a new FISH test. Fifty-eight EFTs were identified in 38 males and 20 females within an age-range of 1-65 years (median, 16), mostly in lower extremities (14) (24.1 %). Therapeutically, most patients underwent neoadjuvant chemotherapy with subsequent surgery. Histopathologically, diagnosis of EFTs was initially offered in 41/58 (70.6 %) tumors. On review, 59 % tumors showed diffuse pattern, while 41 % displayed rosettes. Immunohistochemically, tumor cells were mostly diffusely positive for CD99 (48/52) (92.3 %); FLI-1 (17/18) (94.4 %); variably for BCL2 (16/18) (88.8 %), synaptophysin (6/20) (35 %), S100-P (2/7) (28.5 %), CD56 (2/5) (40 %), NSE (2/5) (40 %), calponin (3/4) (75 %), EMA (5/24) (20.8 %) and CK (3/24) (12.5 %), the latter two mostly focally. Fifty five tumors were EWS-FLI1 positive, while a single tumor was EWS-ERG positive. Sensitivity for PCR was 61 %. EWSR1 rearrangement was detected by FISH in 12/13 Ewing sarcomas/PNETs. Sensitivity for EWSR1 test was 92.3 % and specificity was 100 %. Thirty-eight tumors, including 14 molecular confirmed EFTs and 21 other tumors were tested for EWSR1 rearrangement. Among 21 unrelated tumors, EWSR1 rearrangement was detected in few myoepithelial tumors, occasional desmoplastic small round cell tumor and an extraskeletal myxoid chondrosarcoma. Further, a tissue microarray with a separate set of 8 EFTs, confirmed at

  8. COED Transactions, Vol. IX, No. 3, March 1977. Evaluation of a Complex Variable Using Analog/Hybrid Computation Techniques.

    Science.gov (United States)

    Marcovitz, Alan B., Ed.

    Described is the use of an analog/hybrid computer installation to study those physical phenomena that can be described through the evaluation of an algebraic function of a complex variable. This is an alternative way to study such phenomena on an interactive graphics terminal. The typical problem used, involving complex variables, is that of…

  9. The karyotype of Festucopsis serpentini (Poaceae Triticeae) from Albania studied by banding techniques and in situ hybridization

    DEFF Research Database (Denmark)

    Linde-Laursen, I.; Seberg, O.; Frederiksen, S.;

    1996-01-01

    The karyotypes of two populations of Festucopsis serpentini (2n = 2x = 14) endemic to Albania were investigated in detail by Giemsa C- and N-banding, AgNO3 staining, and in situ hybridization with an rDNA probe. The complements consisted of 14 large chromosomes, 10 metacentric and 4 SAT-chromosom...

  10. Application of optimization technique to noncrystalline x-ray diffraction microscopy: Guided hybrid input-output method

    Science.gov (United States)

    Chen, Chien-Chun; Miao, Jianwei; Wang, C. W.; Lee, T. K.

    2007-08-01

    We have developed an algorithm that combines the concept of optimization with the conventional hybrid input-output (HIO) algorithm for phase retrieval of oversampled diffraction intensities. In particular, the optimization algorithm of guiding searching direction to locate the global minimum has been implemented. Compared with HIO, this guided HIO algorithm retrieves the lost phase information from diffraction intensities with much better accuracy.

  11. FISH studies of chromosome abnormalities in germ cells and its relevance in reproductive counseling

    Institute of Scientific and Technical Information of China (English)

    Zaida Sarrate; Joan Blanco; Ester Anton; Susana Egozcue; Josep Egozcue; Francesca Vidal

    2005-01-01

    Chromosome abnormalities are one of the major causes of human infertility. In infertile males, abnormal karyotypes are more frequent than in the general population. Furthermore, meiotic disorders affecting the germ cell-line have been observed in men with normal somatic karyotypes consulting for infertility. In both cases, the production of unbalanced spermatozoa has been demonstrated. Basically addressed to establish reproductive risks, fluorescence in situ hybridization (FISH) on decondensed sperm heads has become the most frequently used method to evaluate the chromosomal constitution of spermatozoa in carriers of numerical sex chromosome abnormalities, carriers of structural chromosome reorganizations and infertile males with normal karyotype. The aim of this review is to present updated figures of the information obtained through sperm FISH studies with an emphasis on its clinical significance. Furthermore, the incorporation of novel FISH-based techniques (Multiplex-FISH; Multi-FISH) in male infertility studies is also discussed.

  12. FISH and GISH: molecular cytogenetic tools and their applications in ornamental plants.

    Science.gov (United States)

    Younis, Adnan; Ramzan, Fahad; Hwang, Yoon-Jung; Lim, Ki-Byung

    2015-09-01

    The innovations in chromosome engineering have improved the efficiency of interrogation breeding, and the identification and transfer of resistance genes from alien to native species. Recent advances in molecular biology and cytogenetics have brought revolutionary, conceptual developments in mitosis and meiosis research, chromosome structure and manipulation, gene expression and regulation, and gene silencing. Cytogenetic studies offer integrative tools for imaging, genetics, epigenetics, and cytological information that can be employed to enhance chromosome and molecular genomic research in plant taxa. In situ hybridization techniques, such as fluorescence in situ hybridization (FISH) and genomic in situ hybridization (GISH), can identify chromosome morphologies and sequences, amount and distribution of various types of chromatin in chromosomes, and genome organization during the metaphase stage of meiosis. Over the past few decades, various new molecular cytogenetic applications have been developed. The FISH and GISH techniques present an authentic model for analyzing the individual chromosome, chromosomal segments, or the genomes of natural and artificial hybrid plants. These have become the most reliable techniques for studying allopolyploids, because most cultivated plants have been developed through hybridization or polyploidization. Moreover, introgression of the genes and chromatin from the wild types into cultivated species can also be analyzed. Since hybrid derivatives may have variable alien chromosome numbers or chromosome arms, the use of these approaches opens new avenues for accurately identifying genome differences.

  13. Optimized Fast-FISH with a-satellite probes: acceleration by microwave activation

    Directory of Open Access Journals (Sweden)

    Durm M.

    1997-01-01

    Full Text Available It has been shown for several DNA probes that the recently introduced Fast-FISH (fluorescence in situ hybridization technique is well suited for quantitative microscopy. For highly repetitive DNA probes the hybridization (renaturation time and the number of subsequent washing steps were reduced considerably by omitting denaturing chemical agents (e.g., formamide. The appropriate hybridization temperature and time allow a clear discrimination between major and minor binding sites by quantitative fluorescence microscopy. The well-defined physical conditions for hybridization permit automatization of the procedure, e.g., by a programmable thermal cycler. Here, we present optimized conditions for a commercially available X-specific a-satellite probe. Highly fluorescent major binding sites were obtained for 74oC hybridization temperature and 60 min hybridization time. They were clearly discriminated from some low fluorescent minor binding sites on metaphase chromosomes as well as in interphase cell nuclei. On average, a total of 3.43 ± 1.59 binding sites were measured in metaphase spreads, and 2.69 ± 1.00 in interphase nuclei. Microwave activation for denaturation and hybridization was tested to accelerate the procedure. The slides with the target material and the hybridization buffer were placed in a standard microwave oven. After denaturation for 20 s at 900 W, hybridization was performed for 4 min at 90 W. The suitability of a microwave oven for Fast-FISH was confirmed by the application to a chromosome 1-specific a-satellite probe. In this case, denaturation was performed at 630 W for 60 s and hybridization at 90 W for 5 min. In all cases, the results were analyzed quantitatively and compared to the results obtained by Fast-FISH. The major binding sites were clearly discriminated by their brightness

  14. Magneto-optical mode conversion in a hybrid glass waveguide made by sol-gel and ion-exchange techniques

    Science.gov (United States)

    Royer, François; Amata, Hadi; Parsy, François; Jamon, Damien; Ghibaudo, Elise; Broquin, Jean-Emmanuel; Neveu, Sophie

    2012-01-01

    The integration of magneto-optical materials with classical technologies being still a difficult problem, this study explores the possibility to realize a mode converter based on a hybrid structure. A composite magneto-optical layer made of a silica/zirconia matrix doped by magnetic nanoparticles is coated on the top face of ion-exchanged glass waveguides. Optical characterizations that have been carried out demonstrated the efficiency of these hybrid structures in terms of lateral confinement. Furthermore, TE to TM mode conversion has been observed when a longitudinal magnetic field is applied to the device. The amount of this conversion is analysed taking into account the magneto-optical confinement and the modal birefringence of the structure.

  15. Recombinant expression of TLR5 proteins by ligand supplementation and a leucine-rich repeat hybrid technique

    OpenAIRE

    Hong, Minsun; Yoon, Sung-il; Wilson, Ian A.

    2012-01-01

    Vertebrate TLR5 directly binds bacterial flagellin proteins and activates innate immune responses against pathogenic flagellated bacteria. Structural and biochemical studies on the TLR5/flagellin interaction have been challenging due to the technical difficulty in obtaining active recombinant proteins of TLR5 ectodomain (TLR5-ECD). We recently succeeded in production of the N-terminal leucine rich repeats (LRRs) of Danio rerio (dr) TLR5-ECD in a hybrid with another LRR protein, hagfish variab...

  16. [Development of a Dual Detection Method with Fluorescence In Situ Hybridization and Immunostaining on Formalin-Fixed Paraffin-Embedded Tissue Sections--Molecular Pathological Detection Techniques and Their Applications to Pathological Diagnosis].

    Science.gov (United States)

    Ikeda, Satoshi

    2015-06-01

    Fluorescence in situ hybridization (FISH) has recently become important for pathological diagnosis. However, its practical applications is not widespread because FISH protocol with FFPE specimens is complicated. We report a dual detection method by overlapping FISH with fluorescent immunostaining on FFPE sections. This method is characterized by changing buffers for heat treatment without proteolytic enzyme treatment. Subsequent proteolytic enzyme treatment can be omitted using an antigen activation solution, pH9 (Nichirei Corporation), for heat treatment. After the pretreatment, dual detection was achieved by DNA FISH following RNA FISH and fluorescent immunostaining. This protocol visualized gene abnormalities and protein overexpression on the same sections. Of note, in poorly differentiated tumors containing both normal and tumor cells, the tumor cells were clearly identified on the sections, and FISH signals could be counted in these cells. In addition, HER2 mRNA overexpression and gene amplification were simultaneously detected in HER2-positive gastric cancer. Thus, this method should be widely applicable in clinical settings.

  17. International, collaborative assessment of limitations of chromosome-specific probes (CSP) and fluorescent in situ hybridization (FISH): Analysis of expected detections in 73,000 prenatal cases

    Energy Technology Data Exchange (ETDEWEB)

    Evans, M.I.; Henry, G.P.; Miller, W.A. [Wayne State Univ., Detroit, MI (United States)] [and others

    1994-09-01

    FISH and CSP have been proposed to reduce karyotyping need. The purpose of this study was to assess the potential efficacy of CSP-FISH using currently available probes (13, 18, 21, X, & Y) in large, prenatal diagnostic centers. Results (1990-1993) from 7 centers in 4 countries were divided by those expected to be detectable by currently available probes, and those which would be missed assuming 10% probe efficacy. 72,994 karyotypes included 699 trisomy 21`s, 352 trisomy 18`s, 136 trisomy 13`s, 358 sex chromosome aneuploidies, 70 triploidies, and 855 others (translocations, inversions, deletions, markers). Of 2,613 abnormalities, 1,745 would be detectable (66.8%). [Detroit 55.7%, Stockholm 68.3%, Boston 52.6%, Denver 61.3%, Muenster 77.0%, London 84.5%, Philadelphia 69.4%]. Centers with high proportions of referrals for ultrasound anomalies had the highest CSP-FISH positives secondary to increased T 18 & 13. We conclude: (1) 73,000 karyotypes show relatively consistent incidences of the common trisomies, sex chromosome abnormalities, and other chromosome abnormalities among the centers. (2) The proportion expected detectable by FISH-CSP technology varies from 52.6% to 84.5%, averaging 66.8%. (3) 1/3 of the karyotypic abnormalities would be missed, and therefore, replacement of complete karyotyping with FISH would have unacceptably high false-negative rates for routine evaluation. (4) FISH-CSP, while useful when positive for anomalies, is not sufficient when negative to obviate the need for a complete karyotype.

  18. Flow Cytometric Immunobead Assay for Detection of BCR-ABL1 Fusion Proteins in Chronic Myleoid Leukemia: Comparison with FISH and PCR Techniques.

    Directory of Open Access Journals (Sweden)

    Anna Grazia Recchia

    Full Text Available Chronic Myeloid Leukemia (CML is characterized by a balanced translocation juxtaposing the Abelson (ABL and breakpoint cluster region (BCR genes. The resulting BCR-ABL1 oncogene leads to increased proliferation and survival of leukemic cells. Successful treatment of CML has been accompanied by steady improvements in our capacity to accurately and sensitively monitor therapy response. Currently, measurement of BCR-ABL1 mRNA transcript levels by real-time quantitative PCR (RQ-PCR defines critical response endpoints. An antibody-based technique for BCR-ABL1 protein recognition could be an attractive alternative to RQ-PCR. To date, there have been no studies evaluating whether flow-cytometry based assays could be of clinical utility in evaluating residual disease in CML patients. Here we describe a flow-cytometry assay that detects the presence of BCR-ABL1 fusion proteins in CML lysates to determine the applicability, reliability, and specificity of this method for both diagnosis and monitoring of CML patients for initial response to therapy. We show that: i CML can be properly diagnosed at onset, (ii follow-up assessments show detectable fusion protein (i.e. relative mean fluorescent intensity, rMFI%>1 when BCR-ABL1IS transcripts are between 1-10%, and (iii rMFI% levels predict CCyR as defined by FISH analysis. Overall, the FCBA assay is a rapid technique, fully translatable to the routine management of CML patients.

  19. Flow Cytometric Immunobead Assay for Detection of BCR-ABL1 Fusion Proteins in Chronic Myleoid Leukemia: Comparison with FISH and PCR Techniques.

    Science.gov (United States)

    Recchia, Anna Grazia; Caruso, Nadia; Bossio, Sabrina; Pellicanò, Mariavaleria; De Stefano, Laura; Franzese, Stefania; Palummo, Angela; Abbadessa, Vincenzo; Lucia, Eugenio; Gentile, Massimo; Vigna, Ernesto; Caracciolo, Clementina; Agostino, Antolino; Galimberti, Sara; Levato, Luciano; Stagno, Fabio; Molica, Stefano; Martino, Bruno; Vigneri, Paolo; Di Raimondo, Francesco; Morabito, Fortunato

    2015-01-01

    Chronic Myeloid Leukemia (CML) is characterized by a balanced translocation juxtaposing the Abelson (ABL) and breakpoint cluster region (BCR) genes. The resulting BCR-ABL1 oncogene leads to increased proliferation and survival of leukemic cells. Successful treatment of CML has been accompanied by steady improvements in our capacity to accurately and sensitively monitor therapy response. Currently, measurement of BCR-ABL1 mRNA transcript levels by real-time quantitative PCR (RQ-PCR) defines critical response endpoints. An antibody-based technique for BCR-ABL1 protein recognition could be an attractive alternative to RQ-PCR. To date, there have been no studies evaluating whether flow-cytometry based assays could be of clinical utility in evaluating residual disease in CML patients. Here we describe a flow-cytometry assay that detects the presence of BCR-ABL1 fusion proteins in CML lysates to determine the applicability, reliability, and specificity of this method for both diagnosis and monitoring of CML patients for initial response to therapy. We show that: i) CML can be properly diagnosed at onset, (ii) follow-up assessments show detectable fusion protein (i.e. relative mean fluorescent intensity, rMFI%>1) when BCR-ABL1IS transcripts are between 1-10%, and (iii) rMFI% levels predict CCyR as defined by FISH analysis. Overall, the FCBA assay is a rapid technique, fully translatable to the routine management of CML patients.

  20. 膀胱移行细胞癌各分期分级中荧光原位杂交检测敏感性比较%Evaluation on significance of fluorescence in situ hybridization (FISH) for detection of bladder cancer

    Institute of Scientific and Technical Information of China (English)

    徐秀红; 杜雨; 张道新; 田野

    2011-01-01

    Objective In order to look for a better non - invasive method for diagnosing bladder cancer, the fluorescence in situ hybridization ( FISH ) technique had been applied in diagnosis of bladder cancer. Methods FISH and cytological analysis were performed simultaneously on urine - exfoliated cells from 138 patients with hematuria. Chromosomal abnormalities and gene mutation were determined by FISH with probes for chromosomes 3,7, 17 and pl6. The sensitivity of FISH and cytological examination in diagnosing transitional cell carcinoma of bladder was studied and compared. Results Big and irregular cell nuclei and position effect variegation were seen in some cells ( P < 0. 05 ). In stage Tl bladder cancer, the sensitivity of FISH and cytological analysis was 83 . 6 % and 31.3% respectively, while in stage T2 - T4 , their sensitivity was 100 % and 55. 6 % respectively. Their sensitivity was 36. 3 % and 9.1% respectively with grade Gl versus 95. 2 % and 42. 9 % respectively with grade G2, and the sensitivity was 100 % and 50 % respectively with grade G3 ( P < 0. 05 ) in a comparable manner. There was significant difference in total sensitivity for diagnosis and staging of transitional cell carcinoma of bladder between FISH and cytological examination ( P < 0. 05 ). Conclusion FISH is more sensitive than cytological examination, hence it may be applied as a valuable method for diagnosing transitional cell carcinoma of bladder.%目的 评价荧光原位杂交(FISH)在诊断膀胱移行细胞癌各分期分级中的敏感性.方法 应用荧光标记的3、7、17号染色体及p16基因探针检测138例血尿患者尿液中脱落细胞的染色体及基因畸变情况,根据诊断标准计算FISH诊断膀胱移行细胞癌的敏感性.同时留取上述患者尿液送病理科行尿找瘤细胞检查,并比较两种方法对膀胱移行细胞癌的诊断效能.结果 FISH图像中细胞核大且不规则者更易观察到染色体基因异常情况,并可见具有较多染

  1. Hybrid FOSS Project

    Data.gov (United States)

    National Aeronautics and Space Administration — Armstrong researchers are continuing their efforts to further develop FOSS technologies. A hybrid FOSS technique (HyFOSS) employs conventional continuous grating...

  2. Fish Hearing.

    Science.gov (United States)

    Blaxter, J. H. S.

    1980-01-01

    Provides related information about hearing in fish, including the sensory stimulus of sound in the underwater environment, mechanoreceptors in fish, pressure perception and the swimbladder, specializations in sound conduction peculiar to certain fish families. Includes numerous figures. (CS)

  3. Fish Allergy

    Science.gov (United States)

    ... in a clear and consistent manner, so that consumers with food allergies and their caregivers can be informed as ... the menu, cross-contact with fish is possible. Ethnic ... fish. Avoid foods like fish sticks and anchovies. Some individuals with ...

  4. Analysis of fatty acids in 12 Mediterranean fish species: advantages and limitations of a new GC-FID/GC-MS based technique.

    Science.gov (United States)

    Nevigato, Teresina; Masci, Maurizio; Orban, Elena; Di Lena, Gabriella; Casini, Irene; Caproni, Roberto

    2012-07-01

    When fatty acids in fish are analyzed, results in percentage form (profile analysis) are mostly reported. However, the much more useful results expressed as mg/100 g (absolute analysis) is the main information required. Absolute methods based on calibration curves are of good accuracy but with a high degree of complexity if applied to a great number of analytes. Procedures based on the sequence profile analysis-total FA determination-absolute analysis may be suitable for routine use, but suffer from a number of uncertainties that have never been really resolved. These uncertainties are mainly related to the profile analysis. In fact, most profile analyses reported in the literature disagree about the number and type of fatty acids monitored as well as about the total percentage to assign to their sum so leading to possible inaccuracies; in addition the instrumental response factor for all FAME (fatty acid methyl esters) is often considered as a constant, but this is not exactly true. In this work, a set of 24 fatty acids was selected and studied on 12 fish species in the Mediterranean area (variable in lipid content and month of sampling): in our results, and in these species, this set constitutes, on average, 90 ± 3 % of the total fatty acid content. Moreover the error derived from the assumption of a unique response factor was investigated. Two different detection techniques (GC-FID and GC-MS) together with two capillary columns (different in length and polarity) were used in order to acquire complementary data on the same sample. With the protocol here proposed absolute analyses on the 12 cited species are easily achievable by the total FA determination procedure. The accuracy of this approach is good in general, but in some cases (DHA for example) is lower than the accuracy of calibration-based methods. The differences were evaluated on a case by case basis.

  5. A Hybrid Technique Based on Combining Fuzzy K-means Clustering and Region Growing for Improving Gray Matter and White Matter Segmentation

    Directory of Open Access Journals (Sweden)

    Ashraf Afifi

    2012-07-01

    Full Text Available In this paper we present a hybrid approach based on combining fuzzy k-means clustering, seed region growing, and sensitivity and specificity algorithms to measure gray (GM and white matter (WM tissue. The proposed algorithm uses intensity and anatomic information for segmenting of MRIs into different tissue classes, especially GM and WM. It starts by partitioning the image into different clusters using fuzzy k-means clustering. The centers of these clusters are the input to the region growing (SRG method for creating the closed regions. The outputs of SRG technique are fed to sensitivity and specificity algorithm to merge the similar regions in one segment. The proposed algorithm is applied to challenging applications: gray matter/white matter segmentation in magnetic resonance image (MRI datasets. The experimental results show that the proposed technique produces accurate and stable results.

  6. Fiber-wireless integrated mobile backhaul network based on a hybrid millimeter-wave and free-space-optics architecture with an adaptive diversity combining technique.

    Science.gov (United States)

    Zhang, Junwen; Wang, Jing; Xu, Yuming; Xu, Mu; Lu, Feng; Cheng, Lin; Yu, Jianjun; Chang, Gee-Kung

    2016-05-01

    We propose and experimentally demonstrate a novel fiber-wireless integrated mobile backhaul network based on a hybrid millimeter-wave (MMW) and free-space-optics (FSO) architecture using an adaptive combining technique. Both 60 GHz MMW and FSO links are demonstrated and fully integrated with optical fibers in a scalable and cost-effective backhaul system setup. Joint signal processing with an adaptive diversity combining technique (ADCT) is utilized at the receiver side based on a maximum ratio combining algorithm. Mobile backhaul transportation of 4-Gb/s 16 quadrature amplitude modulation frequency-division multiplexing (QAM-OFDM) data is experimentally demonstrated and tested under various weather conditions synthesized in the lab. Performance improvement in terms of reduced error vector magnitude (EVM) and enhanced link reliability are validated under fog, rain, and turbulence conditions.

  7. Role of immunohistochemistry and fluorescence in-situ hybridization (FISH in the diagnosis of spindle and round cell tumors of the kidney

    Directory of Open Access Journals (Sweden)

    M. Abbas

    2015-09-01

    Conclusion: In summary we advise an immunohistochemical panel for round/spindle cell tumors of the kidney and for unclear cases we advise to add (FISH to get the correct diagnosis, as they are completely different regarding surgical approach and post-operative adjuvant therapy.

  8. The Hybrid Programming Technique Of Calling MATLAB Library Functions From Visual C++%Visual C++调用MATLAB函数库的混合编程技术

    Institute of Scientific and Technical Information of China (English)

    徐治

    2015-01-01

    The Visual C++ and MATLAB hybrid programming technique uses VC++ as the powerful software development tool, and directly calls the immense MATLAB algorithm function library while implementing a sophisti-cated algorithm. This approach can achieve the complementation of merits and greatly reduce the coding time of so-phisticated algorithm. This paper introduces the principle of hybrid programming, then details the MATLAB compiler based techniques of coding and project configuration which calls MATLAB function library from VC++.%Visual C++与MATLAB的混合编程技术是以VC++作为强有力的软件开发工具,而在实现复杂算法时则直接调用MATLAB涵盖很广的算法函数库。这种方式可以做到优势互补,大大减少复杂算法的编码时间。本文介绍了混合编程的基本原理,并详细介绍了基于MATLAB编译器的VC++调用MATLAB函数库的代码编写及项目配置等方面的技术。

  9. Hybrid Brain–Computer Interface Techniques for Improved Classification Accuracy and Increased Number of Commands: A Review

    Directory of Open Access Journals (Sweden)

    Keum-Shik Hong

    2017-07-01

    Full Text Available In this article, non-invasive hybrid brain–computer interface (hBCI technologies for improving classification accuracy and increasing the number of commands are reviewed. Hybridization combining more than two modalities is a new trend in brain imaging and prosthesis control. Electroencephalography (EEG, due to its easy use and fast temporal resolution, is most widely utilized in combination with other brain/non-brain signal acquisition modalities, for instance, functional near infrared spectroscopy (fNIRS, electromyography (EMG, electrooculography (EOG, and eye tracker. Three main purposes of hybridization are to increase the number of control commands, improve classification accuracy and reduce the signal detection time. Currently, such combinations of EEG + fNIRS and EEG + EOG are most commonly employed. Four principal components (i.e., hardware, paradigm, classifiers, and features relevant to accuracy improvement are discussed. In the case of brain signals, motor imagination/movement tasks are combined with cognitive tasks to increase active brain–computer interface (BCI accuracy. Active and reactive tasks sometimes are combined: motor imagination with steady-state evoked visual potentials (SSVEP and motor imagination with P300. In the case of reactive tasks, SSVEP is most widely combined with P300 to increase the number of commands. Passive BCIs, however, are rare. After discussing the hardware and strategies involved in the development of hBCI, the second part examines the approaches used to increase the number of control commands and to enhance classification accuracy. The future prospects and the extension of hBCI in real-time applications for daily life scenarios are provided.

  10. Artifact reduction in multichannel pervasive EEG using hybrid WPT-ICA and WPT-EMD signal decomposition techniques

    CERN Document Server

    Bono, Valentina; Das, Saptarshi; Maharatna, Koushik

    2014-01-01

    In order to reduce the muscle artifacts in multi-channel pervasive Electroencephalogram (EEG) signals, we here propose and compare two hybrid algorithms by combining the concept of wavelet packet transform (WPT), empirical mode decomposition (EMD) and Independent Component Analysis (ICA). The signal cleaning performances of WPT-EMD and WPT-ICA algorithms have been compared using a signal-to-noise ratio (SNR)-like criterion for artifacts. The algorithms have been tested on multiple trials of four different artifact cases viz. eye-blinking and muscle artifacts including left and right hand movement and head-shaking.

  11. Fish Culture Economics and Extension

    Directory of Open Access Journals (Sweden)

    E.N. Ogamba

    2012-12-01

    Full Text Available Fish culture economics and extension was reviewed to enable fish culturist plan effectively before involved in fish culture and practices. The cost and benefits of fish culture need be known before participation in the business. There is need for cross-link between research and the fishing community. Prior to introduction of any new innovation in fisheries extension and evaluation of such programmers, the agency responsible for such exercise should have full knowledge of the existing farming practice/techniques and the reasons behind them. In assessing or evaluating the impact of any new techniques or programmers, consideration should be given to such factors as natural conditions, local infrastructures, socio cultural setting, farmers’ production aims and labor economics. The study reviews the types of feasibility study, a typical feasibility study and report on a fish farm project and detail analysis of culture extension to enable fish culturist plan effectively before involved in fish culture and practices.

  12. Influence of tides and winds on fishing techniques and strategies in the Mamanguape River Estuary, Paraíba State, NE Brazil

    Directory of Open Access Journals (Sweden)

    Dandara M.M. Bezerra

    2012-09-01

    Full Text Available This work was carried out in two small fishing communities, Barra de Mamanguape and Tramataia, Northeastern Brazil. The aim was to study these traditional fishermen's knowledge and perception about tide and wind classifications, as well as their fishing strategies and techniques. Our research methodology involved various techniques: free interviews and semi-structured ones, guided tours and direct observations. The results obtained show the fishermen's classification of the tides according to the phases of the moon: 'breaking tide', 'flushing tide', 'dead tide' and 'big tide' designated technically these last as neap tide and spring tide, respectively. Wind is also an essential factor for the fishermen to make successful catches, and they classify it according to direction: North, South, East, Southeast, Southwest, Northeast and Northwest. The data show that fishermen's knowledge can also be useful in devising plans for management and conservation studies for this estuary.Este trabalho foi desenvolvido junto a duas comunidades de pescadores artesanais: Barra de Mamanguape e Tramataia, Nordeste do Brasil. O objetivo foi estudar o conhecimento e a percepção dos pescadores artesanais sobre a classificação das marés e dos ventos bem como as técnicas e estratégias de pesca. A metodologia empregada envolveu várias técnicas: entrevistas livres, entrevistas semiestruturadas, turnês guiadas e observação direta. Os resultados obtidos junto aos pescadores mostraram a classificação das marés de acordo com as fases lunares em: 'maré de quebramento', 'maré de lançamento', 'maré morta' e 'maré grande', designadas tecnicamente estas últimas como maré de quadratura e maré de sizígia, respectivamente. O vento é também um fator essencial no sucesso da pescaria, eles o classificam de acordo com a direção: Norte, Sul, Leste, Sudeste, Sudoeste, Nordeste, Noroeste. Os dados obtidos nesta pesquisa mostraram que o conhecimento dos pescadores

  13. Hybrid-Thresholding based Image Super-Resolution Technique by the use of Triplet Half-Band Wavelets

    Science.gov (United States)

    Chopade, Pravin B.; Rahulkar, Amol D.; Patil, Pradeep M.

    2016-12-01

    This paper presents a modified image super-resolution scheme based on the wavelet coefficients hybrid-thresholding by the use of triplet half-band wavelets (THW) derived from the generalized half-band polynomial. At first, discrete wavelet transform (DWT) is obtained from triplet half-band kernels and it applied on the low-resolution image to obtain the high frequency sub-bands. These high frequency sub-bands and the original low-resolution image are interpolated to enhance the resolution. Second, stationary wavelet transform is obtained by using THW, which is employed to minimize the loss due to the use of DWT. In addition, hybrid thresholding scheme on wavelet coefficients scheme is proposed on these estimated high-frequency sub-bands in order to reduce the spatial domain noise. These sub-bands are combined together by inverse discrete wavelet transform obtained from THW to generate a high-resolution image. The proposed approach is validated by comparing the quality metrics with existing filter banks and well-known super-resolution scheme.

  14. An enhanced security solution for electronic medical records based on AES hybrid technique with SOAP/XML and SHA-1.

    Science.gov (United States)

    Kiah, M L Mat; Nabi, Mohamed S; Zaidan, B B; Zaidan, A A

    2013-10-01

    This study aims to provide security solutions for implementing electronic medical records (EMRs). E-Health organizations could utilize the proposed method and implement recommended solutions in medical/health systems. Majority of the required security features of EMRs were noted. The methods used were tested against each of these security features. In implementing the system, the combination that satisfied all of the security features of EMRs was selected. Secure implementation and management of EMRs facilitate the safeguarding of the confidentiality, integrity, and availability of e-health organization systems. Health practitioners, patients, and visitors can use the information system facilities safely and with confidence anytime and anywhere. After critically reviewing security and data transmission methods, a new hybrid method was proposed to be implemented on EMR systems. This method will enhance the robustness, security, and integration of EMR systems. The hybrid of simple object access protocol/extensible markup language (XML) with advanced encryption standard and secure hash algorithm version 1 has achieved the security requirements of an EMR system with the capability of integrating with other systems through the design of XML messages.

  15. Implementing a Novel Hybrid Maximum Power Point Tracking Technique in DSP via Simulink/MATLAB under Partially Shaded Conditions

    Directory of Open Access Journals (Sweden)

    Shahrooz Hajighorbani

    2016-01-01

    Full Text Available This paper presents a hybrid maximum power point tracking (MPPT method to detect the global maximum power point (GMPP under partially shaded conditions (PSCs, which have more complex characteristics with multiple peak power points. The hybrid method can track the GMPP when a partial shadow occurs either before or after acquiring the MPP under uniform conditions. When PS occurs after obtaining the MPP during uniform conditions, the new operating point should be specified by the modified linear function, which reduces the searching zone of the GMPP and has a significant effect on reducing the reaching time of the GMPP. Simultaneously, the possible MPPs are scanned and stored when shifting the operating point to a new reference voltage. Finally, after determining the possible location of the GMPP, the GMPP is obtained using the modified P&O. Conversely, when PS occurs before obtaining the MPP, the referenced MPP should be specified. Thus, after recognizing the possible location of the GMPP, the modified P&O can be used to obtain the GMPP. The simulation and experimental implementations for the proposed algorithm are performed with different scenarios of shadowing under different irradiations, which clearly indicate that the proposed method is robust and has a fast tracking speed. Moreover, this work presents the load sizing method for PSCs to avoid controller failure when detecting the GMPP. Additionally, in this paper, the user-friendly method for programming the digital signal processing (DSP via Simulink/MATLAB is presented in detail.

  16. Annual trends in catchability and fish stock assessments

    OpenAIRE

    2003-01-01

    A key assumption of many fish stock assessment models is that catchability is constant over time. We assume here that trends in catchability may occur through fishing power creeping. The tuning fleets, which are prone to fishing power development, may be identified using the "Hybrid" method. A range of catchability trends, including values derived from the "Hybrid" method, is then implemented to standardise the fishing effort of some tuning fleets used in the stock assessments performed by XS...

  17. Study on the diagnostic methodology on bacterial vaginosis with fluorescence in situ hybridization technique%荧光原位杂交技术诊断细菌性阴道病的方法学研究

    Institute of Scientific and Technical Information of China (English)

    吕治; 彭国丽; 王克迪; 苏建荣

    2015-01-01

    目的:建立荧光原位杂交技术诊断细菌性阴道病的方法,为细菌性阴道病的诊断和治疗提供准确的实验室依据。方法设计针对阴道加德纳菌、阴道阿托波菌、普雷沃菌、乳酸杆菌的荧光探针。对临床确诊的33例细菌性阴道病妇女,健康体检的28例健康妇女阴道分泌物涂片进行荧光原位杂交检测,建立积分标准。与 Nugent 评分标准进行比对评估。结果荧光原位杂交及积分评价标准对于细菌性阴道病的诊断敏感度、特异度、阳性预测值、阴性预测值和符合率分别为93.9%、96.4%、96.9%、93.1%和95.1%。结论荧光原位杂交方法较传统的实验室涂片染色方法更加客观反映阴道菌群的变化,是一种较 Nugent 评分更准确的细菌性阴道病实验室诊断方法。%Objective To establish an accurate laboratory method for clinical physicians to diagnose and treat bacterial vaginosis(BV)by using fluorescence in situ hybridization technique(FISH). Methods The application of fluorescent probes for detection of Gardnerella vaginalis ( Gv),Atopobium vaginae(Ato),Prevotella(Pre)and Lactobacillus(Lac)were carried out in examination of vaginal smears of 33 patients with bacterial vaginosis and 28 healthy women by using FISH;integral standard had been established and evaluated with Nugent scoring. R